LOCUS       DAZKIF010000001       109921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZKIF010000001 DAZKIF010000000
VERSION     DAZKIF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 109921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.001"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHOTW_000001"
     CDS             complement(<1..209)
                     /locus_tag="ACHOTW_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9842194.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHOTW_000002"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHOTW_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9842195.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHOTW_000003"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHOTW_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9842196.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHOTW_000004"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHOTW_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9842197.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHOTW_000005"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHOTW_000005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP9842198.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHOTW_000006"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHOTW_000006"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP9842199.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHOTW_000007"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHOTW_000007"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP9842200.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHOTW_000008"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHOTW_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9842201.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHOTW_000009"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHOTW_000009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9842202.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHOTW_000010"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHOTW_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP9842203.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHOTW_000011"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHOTW_000011"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9842204.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHOTW_000012"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHOTW_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP9842205.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHOTW_000013"
     CDS             complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHOTW_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFP9842206.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHOTW_000014"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHOTW_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP9842207.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHOTW_000015"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHOTW_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9842208.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHOTW_000016"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHOTW_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9842209.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHOTW_000017"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHOTW_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP9842210.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHOTW_000018"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHOTW_000018"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP9842211.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHOTW_000019"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHOTW_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP9842212.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHOTW_000020"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHOTW_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP9842213.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHOTW_000021"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHOTW_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP9842214.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHOTW_000022"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHOTW_000022"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP9842215.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHOTW_000023"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHOTW_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP9842216.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHOTW_000024"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHOTW_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP9842217.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHOTW_000025"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHOTW_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP9842218.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHOTW_000026"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHOTW_000026"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP9842219.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHOTW_000027"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHOTW_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP9842220.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHOTW_000028"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHOTW_000028"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP9842221.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHOTW_000029"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHOTW_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP9842222.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ACHOTW_000030"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ACHOTW_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP9842223.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ACHOTW_000031"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ACHOTW_000031"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP9842224.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28336..28503
                     /gene="yhfL"
                     /locus_tag="ACHOTW_000032"
     CDS             28336..28503
                     /gene="yhfL"
                     /locus_tag="ACHOTW_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP9842225.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28799..30136
                     /gene="frlA"
                     /locus_tag="ACHOTW_000033"
     CDS             28799..30136
                     /gene="frlA"
                     /locus_tag="ACHOTW_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFP9842226.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30157..31179
                     /gene="frlB"
                     /locus_tag="ACHOTW_000034"
     CDS             30157..31179
                     /gene="frlB"
                     /locus_tag="ACHOTW_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFP9842227.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31230..32057
                     /gene="frlC"
                     /locus_tag="ACHOTW_000035"
     CDS             31230..32057
                     /gene="frlC"
                     /locus_tag="ACHOTW_000035"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFP9842228.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            32057..32842
                     /gene="frlD"
                     /locus_tag="ACHOTW_000036"
                     /pseudo
     CDS             32057..32842
                     /gene="frlD"
                     /locus_tag="ACHOTW_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            32942..33673
                     /gene="frlR"
                     /locus_tag="ACHOTW_000037"
                     /gene_synonym="yhfR"
     CDS             32942..33673
                     /gene="frlR"
                     /locus_tag="ACHOTW_000037"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFP9842229.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33804..34808)
                     /gene="trpS"
                     /locus_tag="ACHOTW_000038"
     CDS             complement(33804..34808)
                     /gene="trpS"
                     /locus_tag="ACHOTW_000038"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9842230.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(34801..35559)
                     /gene="gph"
                     /locus_tag="ACHOTW_000039"
     CDS             complement(34801..35559)
                     /gene="gph"
                     /locus_tag="ACHOTW_000039"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP9842231.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(35552..36229)
                     /gene="rpe"
                     /locus_tag="ACHOTW_000040"
     CDS             complement(35552..36229)
                     /gene="rpe"
                     /locus_tag="ACHOTW_000040"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9842232.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(36247..37083)
                     /gene="dam"
                     /locus_tag="ACHOTW_000041"
     CDS             complement(36247..37083)
                     /gene="dam"
                     /locus_tag="ACHOTW_000041"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9842233.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(37190..38476)
                     /gene="damX"
                     /locus_tag="ACHOTW_000042"
     CDS             complement(37190..38476)
                     /gene="damX"
                     /locus_tag="ACHOTW_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP9842234.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(38568..39656)
                     /gene="aroB"
                     /locus_tag="ACHOTW_000043"
     CDS             complement(38568..39656)
                     /gene="aroB"
                     /locus_tag="ACHOTW_000043"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9842235.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(39713..40234)
                     /gene="aroK"
                     /locus_tag="ACHOTW_000044"
     CDS             complement(39713..40234)
                     /gene="aroK"
                     /locus_tag="ACHOTW_000044"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP9842236.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(40635..41873)
                     /gene="hofQ"
                     /locus_tag="ACHOTW_000045"
     CDS             complement(40635..41873)
                     /gene="hofQ"
                     /locus_tag="ACHOTW_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP9842237.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(41785..42189)
                     /gene="hofP"
                     /locus_tag="ACHOTW_000046"
     CDS             complement(41785..42189)
                     /gene="hofP"
                     /locus_tag="ACHOTW_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP9842238.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTLDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(42179..42619)
                     /gene="hofO"
                     /locus_tag="ACHOTW_000047"
     CDS             complement(42179..42619)
                     /gene="hofO"
                     /locus_tag="ACHOTW_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP9842239.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(42603..43142)
                     /gene="hofN"
                     /locus_tag="ACHOTW_000048"
     CDS             complement(42603..43142)
                     /gene="hofN"
                     /locus_tag="ACHOTW_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP9842240.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(43126..43920)
                     /gene="hofM"
                     /locus_tag="ACHOTW_000049"
     CDS             complement(43126..43920)
                     /gene="hofM"
                     /locus_tag="ACHOTW_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFP9842241.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            44040..46592
                     /gene="mrcA"
                     /locus_tag="ACHOTW_000050"
     CDS             44040..46592
                     /gene="mrcA"
                     /locus_tag="ACHOTW_000050"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP9842242.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(46757..47317)
                     /gene="nudE"
                     /locus_tag="ACHOTW_000051"
     CDS             complement(46757..47317)
                     /gene="nudE"
                     /locus_tag="ACHOTW_000051"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP9842243.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            47637..49772
                     /gene="igaA"
                     /locus_tag="ACHOTW_000052"
     CDS             47637..49772
                     /gene="igaA"
                     /locus_tag="ACHOTW_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP9842244.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            49837..50505
                     /gene="yrfG"
                     /locus_tag="ACHOTW_000053"
     CDS             49837..50505
                     /gene="yrfG"
                     /locus_tag="ACHOTW_000053"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP9842245.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            50516..50917
                     /gene="hslR"
                     /locus_tag="ACHOTW_000054"
     CDS             50516..50917
                     /gene="hslR"
                     /locus_tag="ACHOTW_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP9842246.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            50942..51820
                     /gene="hslO"
                     /locus_tag="ACHOTW_000055"
     CDS             50942..51820
                     /gene="hslO"
                     /locus_tag="ACHOTW_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9842247.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(51883..53607)
                     /gene="yhgE"
                     /locus_tag="ACHOTW_000056"
     CDS             complement(51883..53607)
                     /gene="yhgE"
                     /locus_tag="ACHOTW_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP9842248.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            53986..55608
                     /gene="pckA"
                     /locus_tag="ACHOTW_000057"
     CDS             53986..55608
                     /gene="pckA"
                     /locus_tag="ACHOTW_000057"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9842249.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(55684..57036)
                     /gene="envZ"
                     /locus_tag="ACHOTW_000058"
     CDS             complement(55684..57036)
                     /gene="envZ"
                     /locus_tag="ACHOTW_000058"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP9842250.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(57033..57752)
                     /gene="ompR"
                     /locus_tag="ACHOTW_000059"
     CDS             complement(57033..57752)
                     /gene="ompR"
                     /locus_tag="ACHOTW_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP9842251.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            57980..58456
                     /gene="greB"
                     /locus_tag="ACHOTW_000060"
     CDS             57980..58456
                     /gene="greB"
                     /locus_tag="ACHOTW_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP9842252.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            58553..60874
                     /gene="yhgF"
                     /locus_tag="ACHOTW_000061"
     CDS             58553..60874
                     /gene="yhgF"
                     /locus_tag="ACHOTW_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9842253.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            61312..61539
                     /gene="feoA"
                     /locus_tag="ACHOTW_000062"
     CDS             61312..61539
                     /gene="feoA"
                     /locus_tag="ACHOTW_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP9842254.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            61556..63877
                     /gene="feoB"
                     /locus_tag="ACHOTW_000063"
     CDS             61556..63877
                     /gene="feoB"
                     /locus_tag="ACHOTW_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP9842255.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            63877..64113
                     /gene="feoC"
                     /locus_tag="ACHOTW_000064"
     CDS             63877..64113
                     /gene="feoC"
                     /locus_tag="ACHOTW_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP9842256.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            64316..65194
                     /gene="rpnA"
                     /locus_tag="ACHOTW_000065"
     CDS             64316..65194
                     /gene="rpnA"
                     /locus_tag="ACHOTW_000065"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP9842257.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(65221..65991)
                     /gene="bioH"
                     /locus_tag="ACHOTW_000066"
     CDS             complement(65221..65991)
                     /gene="bioH"
                     /locus_tag="ACHOTW_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP9842258.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            66029..66712
                     /gene="gntX"
                     /locus_tag="ACHOTW_000067"
     CDS             66029..66712
                     /gene="gntX"
                     /locus_tag="ACHOTW_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP9842259.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            66771..67346
                     /gene="nfuA"
                     /locus_tag="ACHOTW_000068"
     CDS             66771..67346
                     /gene="nfuA"
                     /locus_tag="ACHOTW_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP9842260.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            67707..69023
                     /gene="gntT"
                     /locus_tag="ACHOTW_000069"
     CDS             67707..69023
                     /gene="gntT"
                     /locus_tag="ACHOTW_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9842261.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(69068..71152)
                     /gene="malQ"
                     /locus_tag="ACHOTW_000070"
     CDS             complement(69068..71152)
                     /gene="malQ"
                     /locus_tag="ACHOTW_000070"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP9842262.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRGHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGRYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(71162..73555)
                     /gene="malP"
                     /locus_tag="ACHOTW_000071"
     CDS             complement(71162..73555)
                     /gene="malP"
                     /locus_tag="ACHOTW_000071"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP9842263.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            74167..76872
                     /gene="malT"
                     /locus_tag="ACHOTW_000072"
     CDS             74167..76872
                     /gene="malT"
                     /locus_tag="ACHOTW_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP9842264.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            76939..77217
                     /locus_tag="ACHOTW_000073"
     CDS             76939..77217
                     /locus_tag="ACHOTW_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP9842265.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            77208..77693
                     /locus_tag="ACHOTW_000074"
     CDS             77208..77693
                     /locus_tag="ACHOTW_000074"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9842266.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(77743..78771)
                     /gene="rtcA"
                     /locus_tag="ACHOTW_000075"
     CDS             complement(77743..78771)
                     /gene="rtcA"
                     /locus_tag="ACHOTW_000075"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP9842267.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(78775..80001)
                     /gene="rtcB"
                     /locus_tag="ACHOTW_000076"
     CDS             complement(78775..80001)
                     /gene="rtcB"
                     /locus_tag="ACHOTW_000076"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP9842268.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            80189..81787
                     /gene="rtcR"
                     /locus_tag="ACHOTW_000077"
     CDS             80189..81787
                     /gene="rtcR"
                     /locus_tag="ACHOTW_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP9842269.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(81769..82527)
                     /locus_tag="ACHOTW_000078"
     CDS             complement(81769..82527)
                     /locus_tag="ACHOTW_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP9842270.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(82544..83374)
                     /gene="glpG"
                     /locus_tag="ACHOTW_000079"
     CDS             complement(82544..83374)
                     /gene="glpG"
                     /locus_tag="ACHOTW_000079"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP9842271.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(83419..83745)
                     /gene="glpE"
                     /locus_tag="ACHOTW_000080"
     CDS             complement(83419..83745)
                     /gene="glpE"
                     /locus_tag="ACHOTW_000080"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP9842272.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            83935..85440
                     /gene="glpD"
                     /locus_tag="ACHOTW_000081"
     CDS             83935..85440
                     /gene="glpD"
                     /locus_tag="ACHOTW_000081"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP9842273.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(85655..86257)
                     /locus_tag="ACHOTW_000082"
     CDS             complement(85655..86257)
                     /locus_tag="ACHOTW_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9842274.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(86257..87249)
                     /locus_tag="ACHOTW_000083"
     CDS             complement(86257..87249)
                     /locus_tag="ACHOTW_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9842275.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(87275..88783)
                     /locus_tag="ACHOTW_000084"
     CDS             complement(87275..88783)
                     /locus_tag="ACHOTW_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9842276.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(88908..91355)
                     /gene="glgP"
                     /locus_tag="ACHOTW_000085"
     CDS             complement(88908..91355)
                     /gene="glgP"
                     /locus_tag="ACHOTW_000085"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP9842277.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(91374..92807)
                     /gene="glgA"
                     /locus_tag="ACHOTW_000086"
     CDS             complement(91374..92807)
                     /gene="glgA"
                     /locus_tag="ACHOTW_000086"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP9842278.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(92807..94102)
                     /gene="glgC"
                     /locus_tag="ACHOTW_000087"
     CDS             complement(92807..94102)
                     /gene="glgC"
                     /locus_tag="ACHOTW_000087"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP9842279.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(94120..96093)
                     /gene="glgX"
                     /locus_tag="ACHOTW_000088"
     CDS             complement(94120..96093)
                     /gene="glgX"
                     /locus_tag="ACHOTW_000088"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP9842280.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(96090..98276)
                     /gene="glgB"
                     /locus_tag="ACHOTW_000089"
     CDS             complement(96090..98276)
                     /gene="glgB"
                     /locus_tag="ACHOTW_000089"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP9842281.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(98549..99652)
                     /gene="asd"
                     /locus_tag="ACHOTW_000090"
     CDS             complement(98549..99652)
                     /gene="asd"
                     /locus_tag="ACHOTW_000090"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9842282.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            99844..100437
                     /gene="yhgN"
                     /locus_tag="ACHOTW_000091"
     CDS             99844..100437
                     /gene="yhgN"
                     /locus_tag="ACHOTW_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP9842283.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(100483..101778)
                     /locus_tag="ACHOTW_000092"
     CDS             complement(100483..101778)
                     /locus_tag="ACHOTW_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP9842284.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(101685..103809)
                     /locus_tag="ACHOTW_000093"
                     /pseudo
     CDS             complement(101685..103809)
                     /locus_tag="ACHOTW_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(103949..105289)
                     /gene="gntU"
                     /locus_tag="ACHOTW_000094"
     CDS             complement(103949..105289)
                     /gene="gntU"
                     /locus_tag="ACHOTW_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9842285.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(105293..105820)
                     /gene="gntK"
                     /locus_tag="ACHOTW_000095"
     CDS             complement(105293..105820)
                     /gene="gntK"
                     /locus_tag="ACHOTW_000095"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9842286.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(105959..106954)
                     /gene="gntR"
                     /locus_tag="ACHOTW_000096"
     CDS             complement(105959..106954)
                     /gene="gntR"
                     /locus_tag="ACHOTW_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP9842287.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(107178..107873)
                     /gene="yhhW"
                     /locus_tag="ACHOTW_000097"
     CDS             complement(107178..107873)
                     /gene="yhhW"
                     /locus_tag="ACHOTW_000097"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP9842288.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(107996..109033)
                     /gene="yhhX"
                     /locus_tag="ACHOTW_000098"
     CDS             complement(107996..109033)
                     /gene="yhhX"
                     /locus_tag="ACHOTW_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9842289.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            109367..109855
                     /gene="yhhY"
                     /locus_tag="ACHOTW_000099"
     CDS             109367..109855
                     /gene="yhhY"
                     /locus_tag="ACHOTW_000099"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9842290.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
BASE COUNT        26544 a        29764 c        27608 g        26005 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcctgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggccggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagatcca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccattt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca agtggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagtggaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaagg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctact ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccgc aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct agcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc ataccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatctacct gcgtggcatc accgtaataa actttgtagc
     8161 catatttgcg catcaggtta acggcgctga tatcccgctc cagcacagta atgcgcattt
     8221 tatttgccat caacaaacga ccaatcacct gcccaaaacg tccaaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat cccgacagag ataaagaaca aaccgagcag caagccttta aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgcct gcaataaacg
     8761 taccgagcgc catcgacagc cccaacgcat ccataaacaa tgcggaaccc aataccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcaataa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gtaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcggtcgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcaagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcaggacat cgctcatcgg
     9661 gtaacgattc aacgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacact
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacggcttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 tggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gctgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgtcgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaat ctggtccccg gttcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagtggtg agataggcct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcgcct attcaacatc tggcacgttt agcgccgcag gaactggagc aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatc gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgaccttgat atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcgact ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgagtttggc catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccggttgat
    12601 gcggcggtta ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagcc gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt acctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg attcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtgcccgg
    14161 cggcaaaatg ggtctggcaa ttgaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcaga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggctgtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctcaatc tggcaaaaca accagacgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc ttgaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atattaacta
    15721 tgcactgcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccttttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccga cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctattggc acttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcgct
    16741 gaaaagttat ttgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcttga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cgcaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga ccttgatgct gattaccacc cttgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggatccg caaccaacgc cactggcctg gcagcgaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaacca gcgttcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag tcagtttatt gttgaacata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat gcacgccgca tccgacatct
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggcgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cctgaccttt gtactgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattaatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacaact
    18421 cacgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgatggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacgc aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttatg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacttcaaag cacgctggta acgagtcagg ttccactacc
    19321 agcgaatgat agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gagatgacaa ctttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca acgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gttttctcta ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgttgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgact cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctgaaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgaaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagcgaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaat gatgctggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccggcac atatggcgtg gtcaattctc gccctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctcatgcc ggacggcact atcgtcgtcc ggccttttcc tctctaaccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aattaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcagaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgagagcatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagcaaag agatctacaa acgccttatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgtc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactatt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggttacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcggcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtactg ccgacaaact gacgcgtacc
    25141 gcaccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccagta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaataacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cctgattttt acgctcggta atttgctcga
    26041 tccatccgta cgccctctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgagctgt tcaccggaca caccatgttc ctcacctttg gggtgaaagc
    26161 gggcaccatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggt ggtggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgtctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgcaat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactccatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc tgccagcttc attatgccgt cgattattga
    27061 ccgctcaccg ctgatggtgg cggtttcctc tggcggtact tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gctgcatctg ggccaggtag cgaaatacgc
    27181 cgggcagtta cgcggtcggg taaaacaaca gttcgccacg atgggtgaac gccgccgctt
    27241 ctgggaaaaa ctgttcgtta acgaccgtct ggcgcagtcg ctggcgaaca acgatcagaa
    27301 agccattact gaaaccaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gtctgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgcggatc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc
    27541 gcaggaggag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggc ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctcagc
    27721 ctattcgggt attccgctca cgcatcgcga ttatgcccag agcgtgcgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggctga atcaggctgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tagcaattgt cgaaaacgga acagcagtca cgcagcgcgt
    27961 gattgacggt acgctcacac aactgggcga actggcgcag caaatgaata gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 atttaatcag ggccagagaa ttctggcctt tttcgtttac aactctcttt atatattcag
    28141 cagataaaaa tataactcat taacaaaaat catttgtttt tgccccgtta tattcaccgt
    28201 tacagcgact tacaaaatta agtcattcac tcactgacgc cgattaaata ttcctgtatc
    28261 gttcttaatg agcagtctac ttatattcat ataatcaatg aatattaatc aatatataat
    28321 acatagggaa tgtaaatgaa caaatttatt aaagttgcac tggtaggtgc agtactggct
    28381 acgttaactg catgtactgg tcatattgaa aaccgtgata agaactgctc ttacgactac
    28441 ctgctgcacc cagcaatttc tatttctaaa atcattggcg gttgcggtcc tactgcacag
    28501 taagtctctg acagaaatca gctaacgctg atttaattgc ctgacgccct cgggattacc
    28561 cgagggcgta tttgtttcta tatccttctt gctccccacc cgtaaaaata ttttcacttt
    28621 aatttcaatt ggataaccac attcatcagc cacagcgcga tcctgtgcga aattatgtga
    28681 tcttcctcca cattacataa catcatatgt tgttatattc atcatgcatt gtcatgtcac
    28741 cttttaaatg actgcaaact ctcccctaca acgtgttgga caagaaaaag gtatcgctat
    28801 gggaagccag gaactccaac gcaagctcgg attttgggcc gttcttgcaa tcgccgtcgg
    28861 gacaaccgtc ggctccggta tttttgtatc tgtgggtgaa gtggcaaaag cggcgggcac
    28921 gccgtggctg acggtgctcg catttgtcat cggcgggtta attgtgatcc cgcaaatgtg
    28981 cgtatatgcg gaactatcca ccgcttatcc ggaaaatggc gcagattatg tttatctgaa
    29041 aaatgccgga agccgaccgc tggctttcct ctccggctgg gccagcttct gggccaacga
    29101 tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc aatcttggct ttttaacgcc
    29161 tatcgatccg ttgctcggta aatttatcgc cgccggatta attatcgcct ttatgctgct
    29221 gcacctgcgc tccgttgaag gcggcgcagc gtttcagacg ctaattacca tcgccaaaat
    29281 tatcccgttc actatcgtca ttggtcttgg gatcttctgg tttaaagcgg agaattttgc
    29341 cgcccctgct accactgcga ttggcgcaac gggcagcttt atggcgctgc tggcggggat
    29401 ctctgccacc agttggtcgt ataccggcat ggcctctatc tgttatatga ccggcgaaat
    29461 taaaaacccc ggcaaaacca tgccacgagc gctgattggt tcctgtctgc tggttctggt
    29521 gctctacacc ctgctggcgc tggtgatttc cggcctgatg cccttcgaca aactcgccaa
    29581 ttctgaaacg ccgatttccg acgccctgac ctggatcccc gcactcggca gcaccgctgg
    29641 gatctttgtt gccatcacgg cgatgatcgt cattcttggt tcgctttcca gctgcgtgat
    29701 gtaccagccg cggctggaat acgcgatggc gaaagacaac ctgttcttca aatgcttcgg
    29761 ccatgtgcat ccgaaataca acacgccgga tgtctccatc attttgcaag gggcgctggg
    29821 gatcttcttc atcttcgttt ccgatctcac cagcctgctg ggctatttca ccctggtaat
    29881 gtgtttcaaa aataccctca ccttcggctc catcatctgg tgtcgtaaac gcgacgatta
    29941 caaaccgctg tggcgtactc cggctttcgg gctgatgacc cccctcgcca ttgcgtcaag
    30001 cctcattctg gtcgcctcaa cctttgtctg ggcaccgatt cccggcctta tctgcgccgt
    30061 catcgttatt gccactggtc tgcctgctta cgccttctgg gcgaagcgta gccgccagct
    30121 caacgctttg tcgtaatgtt atctggagaa aataaaatgt tggatattga taaaagcact
    30181 gtggactttc tggtcaccga aaatatggtt caggaagtgg aaaaagttct cagccatgac
    30241 gttccgctgg tgcacaccat cgtggaagag atggtgaagc gcgacatcga tcgtatttat
    30301 ttcgttgcct gcggatcgcc actcaacgcg gcgcaaacgg cgaaacatct ggcagatcgc
    30361 ttttccgatc ttcaggttta cgccatttcc ggctgggagt tctgcgataa caccccatat
    30421 cgcctcgacg ctcgttgcgc ggtgattggc gtttctgact acggtaaaac cgaagaggta
    30481 atcaaagcgc tggagctggg ccgggcctgc ggcgcactca ctgcggcgtt caccaaacgc
    30541 gcggatagcc cgattacctc ggcggcggaa tttagcattg attatcaggc cgactgtatc
    30601 tgggaaattc atctgctgct ctgctacagc gtggtgctgg agatgatcac ccgcctcgcg
    30661 ccgcacgcgg aaatcggcaa gatcaaaaac gatctcaaac agctgccgaa tgcgctcggt
    30721 tatctggtac gcacttggga agaaaaaggc cgccagcttg gtgaactggc cagccagtgg
    30781 ccgatgattt acacggttgc tgcaggcccg ctgcgcccgc tgggttacaa agaaggcatc
    30841 gtgacgctga tggaatttac ctggacgcac ggctgtgtga ttgagagcgg tgagttccgt
    30901 cacggcccgc tggagattgt cgaaccgggc gttccgttcc tgttcctgct cggcaatgat
    30961 gaaagtcgcc acaccaccga acgcgccatt aactttgtta aacagcgtac tgacaacgtg
    31021 atcgtcatcg attacgccgc aatttcgcaa ggactgcacc cgtggctggc accgttcctg
    31081 atgttcgtgc caatggagtg gctctgctac tacctgtcta tttacaaaga tcacaacccg
    31141 gatgaacgcc gctattacgg tggtctggtg gaatattaat cctctctccc ggcccggcaa
    31201 cgggccggat ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc
    31261 agcggctgcc tattgaacat gcattccgtg atgcaagcga gctgggttac gatggcatcg
    31321 aaatttgggg cggccgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac
    31381 aaatcaaggc gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca
    31441 acggctatcc gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca
    31501 tgatcaagct ggcgatggat atggcaaaag agatgaacgc gggttatacg ctgatttctg
    31561 cggcccacgc gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact
    31621 taagcgaact gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa
    31681 cgccgtatga atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg
    31741 tgccttcgcc gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac
    31801 cggtgatgag ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca
    31861 gcgacggggc cagcgacacg cattacattc ccggcgaagg caaaatgccg ctgcgggaac
    31921 tgatgcgcga tattattgat cggggctatg agggatactg cacagtggaa ctggtaacga
    31981 tgtatatgaa cgagcccaga ctttacgccc gccaggcgct ggaacgcttt cgcgcgctgc
    32041 tgccggagga tgagtgatga aaaccctggc gacaatcggc gataactgcg tcgatatcta
    32101 cccgcaactg aataaagcgt tttctggcgg taatgcggtg aacgtggcgg tgtactgcac
    32161 tcgctacggt ataaagccgg gatgcattac ctgggttggt gacgatgact acggcacaaa
    32221 gcttaagcag gatctcgcca gcatgggcgt cgatatcagc catgtccata cgaaacacgg
    32281 cgttaccgca caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac
    32341 cgaaggcgtg atggccgact ttgccctgag tgaagaggat tacgcctagc tggcgcagta
    32401 tgacattgtg cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc
    32461 tgcgggcaaa cttaccgctt tcgacgtctc cgacaagtgg gacagcccgc tctggcagac
    32521 actggtgccg catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg
    32581 tctgaagatg aaagcgattg ttgcccgtgg cgcaggcgtg gtgattgtca cgttgggtga
    32641 aaacggcagt attgcctggg atggcgtgca gttctggcgt caggctcctg aacaggtgac
    32701 ggttatcgac accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc
    32761 tgcggggatg acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cacaaactat
    32821 tcagtaccac ggtgcctggt aggcataacg ttggcgtgag catcttcacg ccaacgtgct
    32881 gttacttgcc ggaaaacgac cctataatcc gtgtaattca ttctttattt cagggtcgat
    32941 tatgtcagct acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct
    33001 gctggatgat atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaatga
    33061 gctttgtaca caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt
    33121 ggcagacggc gtactgatcc gctggcaggg caaaggcacc tttgtacaaa gccagaaggt
    33181 cgaaaacgcc ctgctgaccg tcagtggttt taccgatttt ggcgtctcac aaggcaaggc
    33241 gacgaaagag aaagtgatcg agcaagaacg gatcagcgcc gcgccgtttt gcgaaaagct
    33301 caatatcccc ggtaacagcg aagtgttcca tctttgccgg gtgatgtatc tcgataaaga
    33361 gccgctattt attgatagtt cgtggatccc gctgtcgcgt tatcctgact ttgatgagat
    33421 ttacgtcgaa ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt
    33481 cagcgacaaa aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa
    33541 atgcgaactg ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa
    33601 accggtgcac gtctccgaac tcttctgccg cgccaaccgc atcaccttaa caattgataa
    33661 taaaagacat taaccgtaga ccggataaga tgcaccagca tcgcatccgg cgatgccggc
    33721 gcactgaatt ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag
    33781 gggaccgtcc gagcgttttg atattacggc ttcgccacaa aaccaatcgc ttcgtacacc
    33841 gcttttagcg tacgggaagc gtgcgcgctg gctttttctg cgccgtcttt catcacctgt
    33901 tgcaggaagg cttcatcgtt gcggaaacgg tggtaacgtt cctgcaattc agtcagcata
    33961 ccggaaacgg catcagccac ttcgcctttc agatgaccat acatcttgcc ttcgaactgt
    34021 ttttccagtt ccgggatgct ctggcccgtt accgcagaaa ggatatccag caggttggaa
    34081 acgcccgctt tgttctgcac atcgtagcga actaccggcg gctcgtcgga gtcggtgacc
    34141 gcgcgtttga ttttcttcac taccgatttc ggatcttcca gcaggccaat aacgttattg
    34201 cggttatcgt cagacttgga catcttcttg gtcggctcca gcagcgacat tacgcgcgcg
    34261 ccggatttcg gaataaacgg ctccggcacc ttaaagatat cgccgtacag cgcgttgaaa
    34321 cgctgggcga tatcacggct cagttccagg tgctgtttct ggtcttcacc caccggtacc
    34381 agattagttt gatacagcag gatgtccgct gccatcagca ccggatagtc aaacagacca
    34441 gcgttgatgt tctcggcata acgcgcagat ttatctttaa actgcgtcat gcggctcagt
    34501 tcgccgaagt aggtatagca gttcagtgcc cagcctaact gcgcatgttc cggtacgtgg
    34561 gactgaacaa aaatggtgct tttctcagga tcgataccac aagccagata caaggccagc
    34621 gtatccagcg tcgctttacg cagcttctgt gcatcctggc gcacggtgat cgcgtgttgg
    34681 tcaacgatac agtaaatgca atggtagtca tcctgcatgt ttacccactg acgcagcgca
    34741 cccatgtagt taccaatagt caattcacct gagggctgtg cgccactaaa aacgatgggc
    34801 ttagtcattt ttcgattcct gattttcgct atgcggaagc cctaatgcgg gcagaaggtc
    34861 atttatagac tgataaatta catcaggctg gctgagatcg atagcctcgc cgtagttata
    34921 tccgtaggtt aagccaactg atgggcaacc tgccgctttt gctgcctgaa tatcattgcg
    34981 tgagtcgcca acaaacagca tctgttgtgg agcaattccc atccgctcag ccaccagtaa
    35041 cagcgggtcc ggatgcggtt ttttgttttg cacatcatca ccaccaatca ccacgctgaa
    35101 gtatttggcg atatctaagg cttcgagcag cggcgcgacg aacggcgtcg gtttgttggt
    35161 gaccaggcct agcggcaggc ctttagcctg caacgcgccc aacgtatcgg caacgtgcgg
    35221 gaacaaaaac gtcccctctt cggcaacctc gccatagtaa cgatcgaaca gtttacgcag
    35281 aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg ggcggtttac ccattgtttt
    35341 acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg cgctccatca gaacatctgc
    35401 gccgttacca atccaggtaa taacgcgttc ttcacctgcg acgggcaact ccagcgcata
    35461 cagcgccata tctaccgcag cagcaagacc gggagcactg tccaccagcg taccgtcgag
    35521 atcaaaagcg acgccgcgaa tatcttcaaa cttactcatg acttaccttt gccagttcac
    35581 tgcgcatttc atcaatgact tttttgtagt ctggctggtc gaagattgcc gaaccggcaa
    35641 cgaacatatc cgcgcccgcc gcggcgattt cgccaatgtt gttcaccttc acgccaccgt
    35701 ccacttccag acgaatgtca aagccagact cgtcgatacg acgacgtact tcgcgcagtt
    35761 tatccagtgt ttgaggaatg aaagattgac cgccgaaacc agggttgacg gacatcagca
    35821 ggatcacatc cagcttatcc atcacgtaat ccagatagct cagaggtgtc gccgggttaa
    35881 ataccagacc cgctttacag ccattttctt taatcagttg cagcgtgcgg tcaacatgct
    35941 cggaggcttc tggatgaaag gtaatgatgc tggcaccagc ggcagcgaaa tcaggcacaa
    36001 tgcgatcgac cggtttcacc atcaggtgta cgtcgatagg ggcggtaatg ccgtagttac
    36061 gcaaggattt cagcaccatt ggcccaatcg tcagattggg aacgtagtgg ttatccatga
    36121 cgtcaaaatg cacgacatca gcgccagctg ccagggcttt tgcggtatct tcacccaggc
    36181 gggcaaaatc agccgacaga attgaggggg caatcaaata ctgtttcatc cgcttctcct
    36241 tgagaattat tttttcgcgg gtgaaacgac tcctggtttg tacaaagcca gcagttcgtc
    36301 caccttttta cgtgtgccgc cgttgctgct tatactgcgt cgaactttga cgacatgcaa
    36361 ttttgcgcgc tgataccact cacgcgttaa catcgtatcg tgattggaga tcagcacagg
    36421 aatatggcgc tcaaccagac cttcggcgat ctccgccaga tgcgcttgtt gttcaagcgt
    36481 aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca gacagcggtg cataaggtgg
    36541 atcgcaatag acgacagatg catcatctgc gcgcgccatg ctatcggcgt aagactcaca
    36601 atagaaaaag gcattctgcg ctttttcagc gaagtgatac aactctgctt ccgggaaata
    36661 gggttttttg tagcggccaa acggcacatt aaactcaccg cgcagattgt aacgacacag
    36721 gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc gcccgacgga acggatcctg
    36781 gcttttgttg aactcttcgc ggaactgata gtaaacctcg gcgcaatttg tttcgggaac
    36841 aaacagctcg cgtgcggcct gtacgtactc atcagtacgc atcttcacaa tgttatagag
    36901 actgatcagg tcgctattga tatcggcaag gatataacga gaaaagtcgg tgttgagaaa
    36961 caccgacccg gcacctacaa aaggctcaac cagacattcg cccttgggca aatgccgttt
    37021 aatatcatca agcaggggat acttgccccc tgcccacttc aaaaaagcgc gatttttctt
    37081 catgctgact aactaattac accttctccg gctgtggaga aagctccgac agcatccagc
    37141 gcttcaggca actcccgcaa cgataaacca ttgcgggaga taaccttgat tacttcagat
    37201 cggcctgtac ctgacgcagc ggtttcgccc acgggttttt ggcctgaaca tctgctggca
    37261 atgtagatac cgcttttttc gcctcttctt tcgaagcgta cacgccagaa accaggacat
    37321 accacggctg accattacgc gtcgtttcat agacaacgta gtttttcaga ttctctttct
    37381 tcgcccaacc gttcaggttg tcgtagttag aggaactgct cagctgcaga gtgtaatggc
    37441 tggacggtgc cgatttcaac gaacccacat tacctgcggt cttccctcca gcggctggtg
    37501 ttgccgtggt ttgcgccggg gatgccgtct gtactggagc cgtagtcgcc gtctcttttg
    37561 gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc cacaggagca gctggttcag
    37621 tacgcttcgg cgtctgtgct accggcttcg gttccgtttt cacggttgct tgcggttttt
    37681 tcggttcaat caccgcctgc tgacgagctg gacgcgtagt ggccggacgt tcagcagttt
    37741 gcgttttcgc cgtgtcacgc gatgcattgc cattgcgtac tggcgcgaca gttgctggtt
    37801 cagtcggcaa tgtagaattg accgccacat tgttcaactg ttgctgattt tgtggctggg
    37861 tcagcgcatt gttcaggtca ccctgcactt caacacgttg ttgaccatcc gttgccgccg
    37921 gggtttgccc ttgagtcggc gtagaagaga tcggtggcag agaaacatcc tgctgagtat
    37981 tttccgcaga cgtggttccc ggcgctggct gcacgccatt cgcctgatcg gtcgcattgc
    38041 cagcaagatc aatactcttc tcgccagacg cggtttgatc gctggaagtg gtcgaggggg
    38101 cttttagcgc agaaccgata ccgatgatca acagcagtag aaccagaatg ccgacgccca
    38161 tcatcatata ctgacgagaa gcgggtttac tggctgcttt tttgcgctta cgcggacgac
    38221 gctctacgcg ctcttcatcc acggtttcat cttcggattc gtcaatttct tcttcgattt
    38281 ccggttcctc attgcgctct ttttgcgcac gagtcggacg gcgatcgtca gtgtcatcaa
    38341 gttcaatatc atcaaaattg atctgcggtt cgccacgttc agtacgctca gaacgttcag
    38401 aagattgacg agaacgacca gtacgacgat cgctgggatc gggtttcagc tcgtcttctg
    38461 gtttgaattc atccatttaa caccccacta aaaggttaat gcttaccacg ttgcaattaa
    38521 cctgaagcta atagaccgct tgataagcgg cctgaccttt cttgttgtta cgctgattga
    38581 caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc cgctgcgaac ttcactctta
    38641 ccaattgcca acggaagaat taagcgcatc tctcccgcaa ggactttctt gtcacgcagc
    38701 atatgcggta aatacgcctg cgcggacatt tcgcgcggcc cattgaccgg taacccagcc
    38761 cgcgtgagca gagttataat acgctgcgtt tcggcagaac taaactgccc gagacgttcc
    38821 gacgtccgcg ccgccatcac catacccgca gcgaccgctt caccatgtaa ccaattgcca
    38881 taccccattt cagcttcaat ggcatgacca aaggtgtgtc ccagattcag taaagcacgt
    38941 aacccggttt cgcgctcgtc ggcggcgaca acttctgcct tcagttcaca acaacggcga
    39001 atacagtacg ccattgccgg accgtccaga cgcaacaacg catccagatt ctcttccagc
    39061 cagttaaaaa acgtaccgtc aagaataatg ccgtatttga tgacttctgc cagccccgac
    39121 gctaactcac gcgggggaag cgttttcaga cagtcgagat ccaccaccac tgaagcaggc
    39181 tggtagaacg cgccaatcat gtttttaccg aggggatggt tgaccgcagt tttgccgcca
    39241 acggaggaat cgacctgcga cagtaacgtc gtcgggactt gaatgaaacg aacaccgcgc
    39301 tgataactcg ccgccgcgaa gccggtcaga tcgcccacta cgccgccgcc aagcgccacc
    39361 agcgtagtat cgcgaccatg cggcttttgt aacaacgccg taaagacggt atcgagtaca
    39421 gccaggcttt tatactgctc gccgtcagga aggataacgc tatcgacgtt aacacccgcc
    39481 tgttcaagta cgccgcggac cttatcgaga tacagaggag ccagggtttc gttggtgacc
    39541 aacatgacct gctcgcccga tttcagcggt aagaatgaag ctggttcatt aaacaaacca
    39601 gatgcgatgg taattgggta actacgttcc ccgagagtaa cgacaatcct ctccataacg
    39661 cgacatccac cttaattact gtacccgcag acgagtgtat ataaagccag aattagttgc
    39721 tttccagcat gtgaataatc tggtttgcaa ccactttagc gctttgatca tcagtacgaa
    39781 tggtcacgtc ggcaatctct tcatacagcg gattgcgttc attggccaac gcttccagaa
    39841 cttcacgcgg cggtgtttca acgtgcagca acgggcgttt tttatcacgc tgcgtgcgtg
    39901 caagttgctt ttcgatcgtc gtttcaagat aaacgacaac gccacgagcg gaaagacggt
    39961 tacgcgtttc acgggatttc acagagccgc cgccagtagc cagcacaata ccctgtttct
    40021 cggtcaactc attgatgacc ttttcttcgc gatcgcggaa gccttcttcg ccttctaaat
    40081 cgaaaaccca gcccacatca gctccggttc gtttctcaat ctcttgatcg gaatcgtaaa
    40141 attccatatt gagttgttga gctaactggc gcccaatagt gctttttccg gcacccatag
    40201 gcccaaccag aaagatattg cgtttctctg ccattttttc ggtactacta agactattcg
    40261 ttaatgataa acccgcttcg ctcagagagc tccgcaggac atgaactgaa acctcataag
    40321 atattgcgag agtcagactg aaaattatct caatactcca gcgggtttgg caactgaata
    40381 aatcacccat ccctctgcat atctggtcgc tgcaagcgcg ctgccttgct accaccgctc
    40441 tggcgataaa tcaccgggta agattagcgt aaaaaagaca gcaaaatgcc gcctgaatga
    40501 taaatcatca tcatggaagg caggcagcga agagcaccaa ctctcaaatc ggtactcctt
    40561 gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg ttcagcgtaa aaacattacc
    40621 gcttttacgg ctgtttactc actggaaacc agtcgtggcg tgataaacac cactaactcg
    40681 cgtcgttcat cttcttttcc gtcatgacga aataattgcc cgaaccaggg aatgtcgcca
    40741 agcaacggca cgctatcctg acccgattta tttttacggg taaaaatgcc gcccagcgcc
    40801 aacgtttctc cgcttttgac ctcgacctgc gtttcgatct cctgcttatc aatcgccagc
    40861 acttcgccat cggcctgctg tagcacctgc cccggaacgt tctggctgat gtgtaatttc
    40921 agccggatgc gacctttttg taacaccgtg ggcgtgacct ccatccccag gacggcctct
    40981 ttaaattcca ccgacgtcgc gccactttcc ccgctggaaa cctgatatgg aatttcgctc
    41041 ccctgtttaa tgctggcagg ctgaagatgt gaggccagca gacgcggact ggcgataata
    41101 tccagctgct gtttttgttc gagcgcggaa agctcaagat ccagcaaacg tccgttgatg
    41161 cgcccaatgt taaaaccgac atgcgttgtc gccgtcgcta cggagaggtc gctaccaagc
    41221 gtggtgactt gcccaacgcc accagcgtgt tgcgcatcgg ccagcgtcca tttcacgcct
    41281 aactcacgca aacttttttc attaatggtg acaatatgcg ccgacagctc aacctgcccg
    41341 accggcagat ccatttgcgc tacccactgt tcaagggcgc ttaacgccgt tttgttatct
    41401 cgtagcaaaa ggcgattggt gcgtttatcg acggtcatac tccctttggc actcagtagc
    41461 ttctccccag ctttcgccag ctctccggcg tcggcgtatt gcagagtaat attgcgattt
    41521 tccagcggca gatttgcctg cgcccgcgcc tgctccgctt cctgacgggc gatattgtca
    41581 ttctgccagg caatggaatg cactgagaga atgttgccct cctggcgcgt tatcagtccg
    41641 gcgcttttca ctacagtttg tagtgcctgc ttccagggaa catctgttag atgtaacgac
    41701 accgtaccgc tgacgtctgg cgacaccacc aggttcaact tctcctgttc agccagcgcc
    41761 tgcaacacct gagctaccgg aacgtcatcc accatcagcg tcactttttg cggctttgcc
    41821 gcctgtacgc cgggtatcag catcaacagt agtgcggcta tccattgctt catttgtatc
    41881 tccttgccgt tgccacaacc attgtggcgg ttcgcagttt gtcccggtac ccagcgttag
    41941 tacgtctagc gtcagctgta aaattgtcca gccgttttcc agcacatcgt tttgctgcac
    42001 ccgtcgccat ttcttttgcc cgtcttttat tacaccgatg atgcgctcgc ctcgccctac
    42061 catcccctga tagcgccact ggctaagttc gctaatccgg catagatctt ccggcggttt
    42121 aaaagggtca cgcataccgg ttaaaaggca caatgcaata cctgccaaca accagcgttt
    42181 aaccctcatg cggcgtctcc agttgtaggg tgaacaaaag atcatcacct tccacgctta
    42241 atgaaaaacg gctcacgctg acgttgcgct ctgccagccg tgtaaatgcc gacggcactg
    42301 cttcccacag cgttttcaac gccaactcgc ctccctgcgc ggatggatgc cagaaaacca
    42361 gttgtgcgcc gggcaactga aaatccagtg gcgaaaaggg cagcgttttt tcttcgctga
    42421 agggagtggt gtcaaccagg cgatacagtg tggcccaatg ctgatgatga cttgcccgta
    42481 gtcgaattaa tgcgtcgcgc tcttcatggt gtgtcgatga cagaaaaatg agcgtaacta
    42541 acatcagcaa ccagaatgcc cagcaaaact ggcggaggcg gggtgatgtg gcgaaccacc
    42601 agtcaaagaa catgttcatc gctaaccttc cttgttaact gatactcaaa ttgccagcgt
    42661 ccctgcgcat cctgctgcgt ggccccccgc tgattgagat gaaaagatgc atcctggcgg
    42721 agtgacgttt ctagtgcgtt taacgcggta atgcttgttg taagcccctt gatctccagc
    42781 gttccctgct gccagcttat cgttgtcagc caggcgtgct cgggtaaaag cgccgccagt
    42841 gcttccagcg cagattgcca gtcgcgggta aattgtcgct ggcgctgcct ttgcaaacgc
    42901 tgctcacgaa tttgttggcg ctccagcaaa cgtggtttgg ttatctgtaa gctgcgggcg
    42961 agttgttgtt ccccctgaag caaaacggcg tctacgcgag cttcggcgct gcttgtcaga
    43021 cgcagtatta gcgttatccc gacggccagc agcagaggcg caacgaacat cagcaaccag
    43081 aaacgaagaa aagcggtccg gcgttgctgt cgccagggca aaaaattaat tggcgggttc
    43141 atcagtacgc cttcccaagc gccagcccag cgcgatggca aagtctccac ctggcggcgg
    43201 taacggcggc tgacgaacag aaacggcctc ccaaggatca aatccgcctt cgccacatat
    43261 cgcgacgctt tcgggatcaa cggataacgc tgccgccagc tccttcgcgc tagtcatccc
    43321 caccgccagt ttgcgccccc agcgatagcg cgtcgcccac agccactgtt cgttatcacg
    43381 ccaggccaga cattgctgat gagaaggtaa aaaaggcagg aatcgctgta atgcactggc
    43441 atccggggtg atcgcactaa catgaacacg caacctttct gccaaagtaa gcagcgttgc
    43501 cagctctttg ctttgcgcgg cagtcacgtt ataggcggga ctgagtgagt cttcgctgta
    43561 atcgaagcgc agggagtccg gatccatatc cagctcgcga gccatcgtcc cggacagcca
    43621 ggccgtttgc tcccgctcac cgagggacat cgacgggcgc ggaaatgacc gctgtaatgt
    43681 gcgactggcg ggaaacgcca acatgatgtg atggcgctgc ggcagttcgc gactccacgg
    43741 taacaacgtt ttagccagcc gctgcgcatc aacaatccgc ccatctttga taatgtcgtg
    43801 cgccagcggc aaccgccacc agcgttgcaa aaagcattct tttgcgcccc gcacaatagc
    43861 aaccgctacc gcttcttgct gttgtaaatg caaaccaatt tgccagatct taaatgccat
    43921 tgtgatgatc tccttatcac ccgtcactct gacgggtata tcaatgcgtc tggcttgcct
    43981 ttatactacc gcgcgtttgt ttataaactg cccaaatgaa actaaatggg aaatttccag
    44041 tgaagttcgt aaagtatttt ttgatccttg cagtctgttg cattctgctg ggagcaggct
    44101 cgatttatgg cctataccgc tacatcgagc cacaactgcc ggatgtggcg acattaaaag
    44161 atgttcgcct gcaaattccg atgcagattt acagcgccga tggcgagctg attgctcaat
    44221 acggtgagaa acgtcgtatt ccggttacgt tggatcaaat cccgccggag atggtgaaag
    44281 cctttatcgc gacggaagac agccgcttct acgagcatca cggcgttgac ccggtgggga
    44341 tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg gcaagtacca
    44401 ttacccagca gctggcgaga aacttcttcc tcagtccaga acgcacgctg atgcgtaaga
    44461 ttaaggaagt cttcctcgcg attcgcattg aacagttgct gacgaaagac gagatcctcg
    44521 agctttatct gaacaagatt taccttggtt accgcgccta tggtgtcggt gctgcggcac
    44581 aagtctattt cggaaaaacg gtcgaccaac tgacgctgaa cgaaatggcg gtgatagccg
    44641 ggctgccgaa agcgccttcc accttcaacc cgctctactc gatggatcgt gccgtcgcgc
    44701 ggcgtaacgt cgtgctatcg cggatgctgg atgaaggata tatcacccag caacagttcg
    44761 atcagacacg cactgaggcg attaacgcta actatcacgc gccggagatt gctttttctg
    44821 cgccgtacct gagcgaaatg gtgcgccagg agatgtataa ccgttatggc gaaagtgcct
    44881 atgaagacgg ttatcgcatt tacaccacca tcacccgcaa agtgcagcag gccgcgcagc
    44941 aggcggtacg taataacgtg ctggactacg acatgcgcca cggctatcgc ggcccggcaa
    45001 atgtgctgtg gaaagtgggc gagtcggcgt gggataacaa caagattacc gatacgctga
    45061 aggcgctgcc gacctatggt ccgctgctgc ctgccgcagt caccagcgcc aatcctcagg
    45121 aagcgacggc aatgctggcg gacgggtcga ccgtcgcatt gagtatggaa ggcgttcgct
    45181 gggcgcgtcc ttaccgttcg gatactcagc agggaccgac gccgcgtaaa gtgaccgatg
    45241 ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg tggctggcac
    45301 aagtgccgga agtgaactcg gcactggtgt cgatcaatcc gcaaaatggt gccgttatgg
    45361 cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc caggcactgc
    45421 gtcaggtggg ttccaacatc aaaccgttcc tctacaccgc ggcgatggat aaaggtctga
    45481 cgctggcaag tatgttgaac gatgtgccaa tttctcgctg ggacgcaggt gccggttctg
    45541 actggcagcc gaaaaactca ccaccgcagt atgccggtcc aattcgctta cgtcaggggc
    45601 tgggtcagtc gaaaaacgtg gtgatggtac gcgcaatgcg ggcgatgggc gtcgactacg
    45661 ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac accgaatcgc
    45721 tggcgctggg ttcagcgtcc ttcaccccaa tgcaggtggc gcgcggctac gcggtcatgg
    45781 cgaacggcgg cttcctggtg gacccgtggt ttatcagcaa aattgaaaac gatcagggcg
    45841 gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt ccggtgattt
    45901 acggtgatac gcagaaatcg aacgtgctgg aaaataacga tgttgaagat gtcgctatct
    45961 cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca aatcaggcgt
    46021 tagtggcgaa gactggcgcg caggagtacg caccgcacgt catcaacact ccgctggcat
    46081 tcctgattaa gagtgctttg aacaccaata tctttggtga gccaggctgg cagggtaccg
    46141 gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc ggtaccacta
    46201 acagttcgaa agatgcgtgg ttctcgggtt acggaccggg cgttgtgacc tcggtctgga
    46261 ttggctttga tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga gcgattaaag
    46321 atcagatttc aggttacgaa ggcggtgcca agagtgccca gcctgcatgg gacgcttata
    46381 tgaaagccgt tcttgaaggt gtgccggagc agccgctgac gccgccgccg ggtattgtga
    46441 cggtgaatat cgatcgcagc accgggcagt tagctaatgg tggcaacagc cgcgaagagt
    46501 atttcatcga aggtacgcag ccgacacaac aggcggtgca cgaggtggga acgaccatta
    46561 tcgataatgg cgaggcacag gaattgttct gattaaaaag gcgcttcggc gccttttcag
    46621 tttgctgaca aagtgcactt gtttatgccg gatacgggtg aacgcgttat ccggctaaca
    46681 aaatcgtgaa aactcaatat attgcaggaa ccccataagc ctgataagca ttgtgcatca
    46741 ggcaaacttc acgcatttac actcgcccct gccctttcaa ccattcgcgc acgaggaaca
    46801 gcgcgctgac attacgcgct tcgttgaagt cagggtcttc cagcaaatcc atcatatgcg
    46861 ccagcggcca gcgcacctgt ggtagcggct ctggctcatc gccttccagc gattccgggt
    46921 agagatcttg cgctaccacg atattcattt tgctggaaaa gtaagacggt gccatgctga
    46981 gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac ctcttctttt agctcgcggt
    47041 tagcggcttc gaagacgctt tcacccggat caattaatcc tttcgaaaaa cctaattcgt
    47101 aggattcagt tcccactgca tattcgcgga tcaggatcag gtgatcgtcc acaatcggca
    47161 caatcatcac tgcttcccgg ttggttggac gcattcgttc ataaacacgc cgcacgccat
    47221 tgctgaactc tagatccacg ctctcgacgg taaacagtcg ggaacgggct acagtttcaa
    47281 cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt gggatctatg ctgtgaaatc
    47341 agcggttatt gtgcgatatc ggacacgctt tcggcaatgt gaattgcatg ttatttacat
    47401 ttatgtaact taataaataa tcgtcctcaa atcaaattaa aagtcaatag gttgaaataa
    47461 ctccaggaat ttgctgatat tccgccttca gagggtttgc tatgatcagc ggttactgtg
    47521 atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg atagccaacc gcagaatcat
    47581 gtattgtgtc cggtgcgact gaccacgcct gacagactaa gtaagatggg gaaagcatga
    47641 gcaccattgt gattttttta gctgctttgc tggcctgctc actacttgcg ggatggctga
    47701 taaaagtgcg atccagacgg cgtcagctgc cctggaccaa cgccttcgcg gatgcgcaaa
    47761 cgcgtaaact cacacctgaa gaacgtagcg ccgttgaaaa ttatcttgag agcctgacgc
    47821 aggtattaca ggtgcctggc ccaacgggag ccagcgcggc accgatctct ctggcgctga
    47881 atgccgaaag caacaacgtc atgatgctga cacacgctat cacgcgttac ggcatctcta
    47941 ccgacgatcc gaataaatgg cgttactacc tcgattcggt agaagtccac ctgccccctt
    48001 tctgggagca gtacatcaac gatgagaata ccgttgaact gattcatacc gattcgctgc
    48061 cgctggttat ttcactcaac ggtcatacgc tgcaggagta catgcaggaa actcgcggct
    48121 atgccttgca acctgttccg tccacgcagg cgtcgattcg cggggaagag agtgagcaaa
    48181 tcgagctact caatattcgc aaagaaacgc atgaagaata tgcgctgagt cgtccgcgcg
    48241 ggctgcgtga agcgttgctg atcgtcgcct ccttcctgat gttctttttc tgcctgatta
    48301 ccccggatgt atttgttccg tggctggcag gcggcgcgtt actgctgctg ggcgcaggtc
    48361 tgtgggggct attcgccccc ccggcaaaat cctccctgcg ggaaattcat tgtctgcgcg
    48421 gtacaccccg tcgttgggga ttgtttggcg aaaacgatca ggaacagatc aacaatattt
    48481 cactcggtat tatcgacctg gtctatcccg cacactggca gccatacatt gctcaggatc
    48541 tcggtcaaca aaccgatatt gatatctatc tcgaccgcca cctagtgcgt caggggcgat
    48601 atctttcgct gcatgatgaa gtaaaaaact ttccgttaca gcactggctg cgcagtacga
    48661 ttatcgctgc gggctcgctg ctggtgctgt ttatgctgtt attctggatc ccgctggata
    48721 tgccgctgaa attcactctc tcatggatga aaggtgcgca gaccattgaa gccaccagcg
    48781 taaaacaact ggctgacgct ggcgtgcggg taggcgatac attgcgtatt agcggtacgg
    48841 gaatgtgtaa tattcgcact tccggaacct ggagcgcgaa aaccaattca ccttttttac
    48901 cgtttgactg ctcgcagatc atctggaacg acgcccgctc attaccatta ccagaatctg
    48961 aactggtcaa caaagcgacg gcattgactg aagcagttaa tcgccagctg caccctaaac
    49021 cggaagatga atctcgcgtc agcgcctcat tacgttcagc aattcaaaaa tccggcatgg
    49081 tattgcttga tgattttggc gacattgtac tgaagacagc ggatctgtgt tctgccaaag
    49141 atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa gactgggacg
    49201 cgctggtaaa acgcgccaac gccgggaagc tcgatggcgt gaatgtgtta ttacgcccgg
    49261 tgagtgcgga atcgctggat aacctggtgg caacctccac cgcgccgttt atcacgcatg
    49321 aaacggcgcg agcggcacaa tcactaaaca gtccggcccc cggcggattc ctgattgtca
    49381 gcgacgaagg cagcgatttt gttgatcagc cctggccttc ggcatcactt tacgactacc
    49441 cgccgcaaga acagtggaac gctttccaga aactggcaca aatgctgatg catacgccgt
    49501 ttaacgccga gggtatcgtc acaaaaatct tcactgacgc caatggtacg cagcatatag
    49561 gccttcatcc gatcccggat cgttccggcc tgtggcgcta tctcagcacc acgctgctgc
    49621 tactgacgat gctgggtagc gccatttata atggcgtaca ggcctggcgg cgttaccagc
    49681 gtcatcgcac tcgcatgatg aagattcagg cctattatga aagctgcctg aacccgcaac
    49741 tgatcacccc ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt tatgctaccc
    49801 tgtcgcgcaa attgcttcac tctggagatt ttcctcatgc atatcaacat tgcctggcag
    49861 gacgtagata ccgttctgct ggatatggac ggcacgttgc tcgacctcgc cttcgataac
    49921 tatttctggc aaaagctggt gcctgaaaca tggggcgcga aaaacggggt tacgccacag
    49981 gaagcgatgg aatatatgcg ccagcaatat cacgacgtac agcatacgct aaactggtac
    50041 tgtcttgatt actggagtga gcaactgggt ctggatatct gtgcgatgac caccgagatg
    50101 ggaccgcgtg ccgtactgcg tgaagatacc attccgtttc ttgaggcact gaaagccagc
    50161 ggtaagcagc gaattttgct caccaatgcg catccgcaca acctggcggt aaaacttgag
    50221 cataccggtc tggacgcaca ccttgattta ttactttcca cccacacatt tggttatccg
    50281 aaagaggatc agcggttatg gcatgcggtg gccgaagcta cgggtctgaa agctgaaaga
    50341 acgctgttta ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt tggtattcgt
    50401 tactgcctcg gcgtgactaa tcctgactca gggattgccg agaaacagta tcaacgccat
    50461 ccgtcactga atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag acgccatgaa
    50521 agaaaaacct gctgttgagg ttcgactgga taaatggcta tgggctgccc gtttttataa
    50581 aacccgcgcg ctggcccgtg aaatgattga aggcggtaag gtgcattaca acgggcagcg
    50641 cagcaagccg agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc agggaaatga
    50701 cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt cgccccgcca gcgaggcagc
    50761 cttgctgtat gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg cgctggcacg
    50821 taaacttaat gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa aagagcgccg
    50881 cgacctgtta cgatttaaac acggcgacag tgaataactg tcacctgcaa gagagatgat
    50941 tatgccgcaa catgaccaat tacatcgcta tctgtttgaa aactttgccg tgcgcggcga
    51001 actggtaacc gtttcggaaa ccctgcaaca gatccttgag aatcacgatt atccgcagcc
    51061 cgttaaaaac gtgctggcag aactgctggt tgcgaccagc ctgttaaccg ctacgctgaa
    51121 gtttgatggt gatatcaccg tacagctgca aggcgacggt ccgatgaatc tggcggttat
    51181 taacggtaac aacaaccagc agatgcgcgg tgtggcgcgc gtgcagggcg aaattccaga
    51241 aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca ttaccccgag
    51301 cgaaggtgaa cgctatcagg gcgtggtggg tctggaaggt gataccctgg cggcctgcct
    51361 ggaagattac tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc gcaccggcga
    51421 cgtagacggc aaaccggctg caggcggtat gttgttgcag gtaatgcctg cgcaaaatgc
    51481 ccagcaggac gactttgacc acctggcgac gctaaccgaa accatcaaaa ccgaagaact
    51541 gctgacctta ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag aggtgacggt
    51601 ttacgatccg caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt gcgccgatgc
    51661 gctgaaaacg ctgcctgatg aagaagtcga tagcatcctg gcggaagatg gcgaaattga
    51721 catgcattgt gattactgcg gtaaccacta tctgttcaat gcgatggata ttgctgaaat
    51781 ccgcaacaac gcgtctccgg cagatccgca agttcattaa tgagtatgtc cggcagagag
    51841 agctctgccg gattttacga ctaatacacc tgatgcgctt tgtcactcat tcagattcac
    51901 atccagcctt tcaccatcca ctacaagatc gcgccacgct ttaggcttcg ttcccagttt
    51961 tccatccttc gcagctgtga gtaatttttc tttcgttata tcacgcggaa gtaaactcat
    52021 cgtaagctct tgccattctt tcttgtcagg atcaaagcca tagacaatgt atctttcatc
    52081 atcaaaggca aagaggatcc gctctgccct gccatcacta ttcagatctt gctcgaccag
    52141 tacgcaggcg tctttttcaa tacaggtcat cacgttatag cgatcctgga ttaaggccga
    52201 ccagaatgtc gcatccggtt taccagaacc aggggcaatt aacacatttt cggctaatac
    52261 tttttccgat atctgttgct gaagatgttg ctctccatct aacgccatca gtagatcccg
    52321 cgcgcgtttc gggtctttca tatactcggc atcgcttttc agcgactcaa gcgccgcacg
    52381 tccatagcga ccgctgtgtt cgagcatgta gatagttacc tggtctggcg tgtttttgcc
    52441 gctctgataa cgcgccatat gactgttcac actaatgcgc atactgtcca gcaccggcga
    52501 attaagaagc accagtatta ccaacaccaa taacgaaacc gcaaggttca ctttgccctg
    52561 aagaacaagg ggattttgcc ctttacgcca gacgatactg acaaaatacc ccagcgacca
    52621 taccagtaac accagcaccg ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg
    52681 agcgacccgc agccataacg cccaggcggc gatgaacaca tacagcggag caaccagcag
    52741 agcggtttta atcaggcaac gcaacggccc cgtccaggga agtgacgctt tttgcggatc
    52801 gcggacaata gccattaaga tcaattgcaa aaaggccagc gttaacagca acccggcggc
    52861 ggagatgtgg cgagaaatcg cactcaggcc tgtaaacggc agggtgatga taaacatcag
    52921 ggttagcaat gatactaacg gcagcaaccc cgtggcgatt aacgtgaaca acttttgaat
    52981 agagtcgatt aaacgtgact gtgttcgcgc gaggatcacc gccagcgcgg taaccagacc
    53041 taacgtgaga taaataaacc agtcggttgc aaaaaagagc gttttgaaaa acgtgatgcc
    53101 aacaagttta aacaactcac tccacagtaa aagcaccagc cacgtcagac cattggcgag
    53161 aaaaatcacc agtaatataa gtacgttatg ccatactgac tggtaaaaat agcgatagcg
    53221 gctactgtca ttgcggatgc gaaggctttg ttgtatccac ggtagcaaca acattgccat
    53281 cagcagcaga tagcaaccaa aatcccaaag cgccttttca gctctccagg gggtcatgcc
    53341 atcagtttgc cacttcagcc agccgctcat ccccagcgtg gcaataaaca ccagcgccag
    53401 ccatccccaa aggcgcttct gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa
    53461 cgcgacggtc gcaggcacgc cataaacaat ccaactgtga ttttttccgg aaagccaggc
    53521 gatcagcagg tagcaaacca gaccctgaag taatccggta gcaatcatcc cccagcgtgt
    53581 tgcgggtgaa agttcaacgt tatccatgtt ggttatccag aatcaaaagg tgggttaatt
    53641 atcgcatccg ggcagtagta ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg
    53701 gtgaatcgat actttaccgg ttgaatttgc atcaatttca ttcaggaatg cgattccact
    53761 cacaatattc ccgccatata aaccaagatt taaccttttg agaacatttt ccacacctaa
    53821 aatgctattt ctgcgataat agcaaccgtt tcgtgacagg aatcacggag ttttttgtca
    53881 aatatgaatt tctccagata cgtaaatcta tgagccttgt cgcggttaac acccccaaaa
    53941 agactttact attcaggcaa tacatattgg ctaaggagca gtgaaatgcg cgttaacaat
    54001 ggtttgaccc cgcaagaact cgaggcttat ggtatcagtg acgtacatga tatcgtttac
    54061 aacccaagct acgacctgct gtatcaggaa gagctcgatc cgagcctgac aggttatgag
    54121 cgcggggtgt taactaatct gggtgccgtt gccgtcgata ccgggatctt caccggtcgt
    54181 tcaccaaaag ataagtatat cgtccgtgac gataccactc gcgatacttt ctggtgggca
    54241 gacaaaggca aaggtaagaa cgataacaaa cctctctctc cggaaacctg gcaacatctg
    54301 aaaggcctgg tgaccaaaca gctttccggc aaacgtctgt tcgttgtcga cgccttctgt
    54361 ggtgcgaacc cggatactcg tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg
    54421 cattttgtca aaaacatgtt tattcgcccg agcgatgaag agttggcagg tttcaaacca
    54481 gactttatcg ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga acagggtctg
    54541 aattccgaaa acttcgtggc ttttaacctg accgagcgca tgcagctgat tggcggcacc
    54601 tggtatggcg gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg
    54661 aaaggtatcg cttctatgca ctgctccgcc aacgttagcg agaaaggcga tgttgcggtg
    54721 ttcttcggcc tttccggcac cggtaaaacc accctttcca ccgacccgaa acgtcgcctg
    54781 attggcgatg acgaacacgg ctgggacgat gacggcgtgt ttaacttcga aggcggctgc
    54841 tacgcaaaaa ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt
    54901 cgcgatgcgc tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat
    54961 ggttcaaaaa ccgagaacac ccgcgtttct tatccgatct atcacatcga taacatcgtt
    55021 aagccggttt caaaagcggg tcacgcaacg aaagttatct tcctgaccgc agacgccttt
    55081 ggtgtgctgc caccggtttc tcgtctgact gccgatcaaa cccagtacca cttcctctct
    55141 ggcttcaccg ccaaactggc cggtactgag cgtggcatca ccgaaccgac gccaaccttc
    55201 tccgcttgct tcggcgcggc attcctgtcg ctgcacccga ctcagtacgc agaagtgctg
    55261 gtgaaacgta tgcaggcggc gggcgcgcag gcttatctgg ttaacactgg ctggaacggc
    55321 actggcaaac gtatctcgat taaagatacc cgcgccatta tcgacgccat cctcaacggt
    55381 tcgctggata atgcagaaac cttcactctg ccgatgttta acctggcgat cccaaccgaa
    55441 ctgccgggcg tagacacgaa gattctcgat ccgcgtaaca cctacgcttc cccggaacag
    55501 tggcaggaaa aagccgaaac cctggcgaaa ctgtttatcg acaactttga taaatacacc
    55561 gacacccctg cgggtgccgc gctggtagcg gctggtccga aactgtaatg atttgaagct
    55621 ggagaatatc tatccagtat cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt
    55681 tatttaccct tcttttgtcg tgctctgcgc ccgcgttacc ggcacaggca gccaggcgcg
    55741 aatggaaagc ccgccccgct cgctggtgcc aagctccagc atcccgttat ggttatccac
    55801 gatacgctgc acaatcgcca gccctaatcc cgtgccgcta atggtgcgtg cactgtcgcc
    55861 gcgaacaaac ggctggaaaa ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc
    55921 ttccacctgg aaccaggcgc gattcggctc cgtcccgctg ctgactttga tccagccatt
    55981 gccgtaacgg gcggcgttga ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg
    56041 cattttcact tcaatgctgc cggggtaaag cgcggtttca atttcccgct catagccact
    56101 ttcggcagca ataacctcac cgagtactgc gttgagatcc gccatttcca tcggcatctc
    56161 ctgtccggtg cgcaggtagt cgataaactg ctcaatgatg gcattgcact cctcgatatc
    56221 tttattgatc gattctgcca ggtagccatc ctgctcgctc atcatctcgg tcgccaggcg
    56281 aatacgcgtc agcggtgtac gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc
    56341 atccgccagt tgcttaacac cagccgccat atggttaaag gcgcgggtaa cggaacgcac
    56401 ctcggacgcg ccatactcac gcagcggcgg cggaataatc cctttaccaa cctgcaaggc
    56461 tgcgtgttcg agatcgacca acggtcgatt ctggatacga ataaacagcc acgccccgcc
    56521 tatcgccaat agcataatcg ccaacgtata gcggaacagc ggagagaaat cgccctgatg
    56581 aatttcggtc agcggcacgc gtacccagat attgggcgac agccaggttt tcagccagac
    56641 gacaggcgaa cttttgttga cctcaacgcg cacttccgtc gggccgccca gttgctgtgc
    56701 catctgatgg cttaagaatt catagtgttg cgcccaacgc agacctgcct cttcggcagc
    56761 ctcgttggag tagagagaga tccccagctc acggtagatc tcccgacgga aagcgggagg
    56821 cacaaccaac tgcgtgccgt cctccagttg cagtttgtcg gtcatcaaca tacgcacttc
    56881 gtacgcgagg actttattaa actgctggag gctcggcaaa atcgcgaagt tcagcaccac
    56941 cagataagtc gtcaccaggc tggcgaacag caaggtgacg atgagcaata acgtacgggc
    57001 aaatgaactt cgtggcgaga agcgcaatcg cctcatgctt tagagccgtc cggtacaaag
    57061 acgtagccca ggccccaaac ggtctgaatg taacgcggat gcgctggatc ttcttccacc
    57121 atgcggcgca gacgagaaat ctgcacgtcg atggagcgtt ccattgcgga atattcacga
    57181 ccacgggcaa ggttcatcag cttatcgcgg gagagcggct cacgcggatg gctgaccagt
    57241 gccttcagta ccgcaaactc accgctggtg agcggcatcg gctcgtcttc gcggaacatt
    57301 tcgcgcgtac cgaggttaag tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac
    57361 ggtgcgcctg gtagctcgtt cgcctgacga cgcagcaccg cacggatacg ggccagcagt
    57421 tcacgcgggt taaacggttt tggaatgtaa tcgtcagcgc caatctccag gccaacgata
    57481 cggtccactt cttccccttt cgccgtcacc ataatgatcg gcatcgggtt gctctgacta
    57541 cgaagacgtc ggcaaatcga caagccatct tcaccaggta acattaaatc cagtaccata
    57601 agatggaaag attcacgagt cagcaggcga tccatctgtt ctgcattagc gacgcttcga
    57661 acctggaagc cttgttcggt gagataacgt tccagcagcg cacgcaggcg catgtcgtca
    57721 tcgaccacca gaatcttgta gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa
    57781 tttgtaagcg tgtattctta aaaaagctca cgttcgtcac cagctaaatc tggtatgaat
    57841 ttcagcctaa attgttacaa agcatattaa acagcagctt aagtatacaa tttattcggc
    57901 gaaacattat tgattctgtt gatatgatca cgttataccc aatgtgcgca ttatcaaaca
    57961 gacaaaggga atcaacgaga tgaaaacgcc cctggttacc cgggaagggt atgaaaaact
    58021 caaacaagag cttaattatc tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac
    58081 ctgggccgca agtctgggcg accgcagcga aaatgctgac tatcagtata ataaaaagcg
    58141 cctgcgtgaa atcgaccgtc gcgtgcgcta tctcactaaa tgcatggaaa atctcaaaat
    58201 cgtcgattac tccccgcagc aggaaggcaa agtctttttt ggcgcgtggg tggagattga
    58261 aaacgacgat ggcgtgactc accgtttccg tattgtcggc tacgatgaaa tttttggccg
    58321 taaagattac atctctatcg attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg
    58381 cgatctggct gtggtgaata ctcctgccgg ggaagcgagc tggtatgtta atgctatcga
    58441 gtacgtgaaa ccgtaaggaa gagtcttaac ctcctgccga tggctggcat ttttgccagc
    58501 cagtccgtat aactatcccc tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa
    58561 tgattcgttc tgccgcatta ttgcgggtga aattcaggcg cgcccggaac aggttgacgc
    58621 tgccgttcgc ctgcttgacg aagggaatac cgtgcctttt attgcacgtt atcgtaagga
    58681 aatcaccggc ggtctggatg acacgcagct gcgtaatctg gaaacgcgtc tgggatacct
    58741 gcgcgagctg gaagagagac gtcaggcgat cctcaagtcc atttccgagc aaggcaaact
    58801 caccgatgat ctggcgaatg ccatcaacgc caccctaagc aaaaccgaac tcgaagacct
    58861 ctacctgccc tacaaaccta aacgccgcac ccgcgggcaa atcgccattg aagcagggct
    58921 tgagccgttg gctgacctgc tgtggagcga tccgtcacac acgccagaag tcgccgctgc
    58981 gcaatatatt gatgccgata aaggcgtggc agataccaaa gccgcgctgg acggcgcgcg
    59041 ctatatcctg atggaacggt ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta
    59101 tctgtggaag aacgcgcatt tggtttctac ggtggtgagc ggtaaagaag aggaaggggc
    59161 gaaattccgc gactatttcg atcatcacga accgttgtcc acggtgcctt ctcaccgcgc
    59221 gctggcgatg ttccgtgggc gtaacgaagg cgtacttcag ctttcgctga atgccgatcc
    59281 acagttcgat gagccgccca aagagagcta ttgcgagcaa atcatcatgg atcaccttgg
    59341 cctgcgcctg aacaatgccc cggcggatag ctggcgcaaa ggcgtggtga gctggacgtg
    59401 gcgcatcaag gtgctgatgc atctggaaac cgaactgatg ggcaccgtgc gcgaacgtgc
    59461 ggaaaatgaa gcaatcaacg tctttgcccg taacctgcac gatctgctga tggctgcccc
    59521 tgccggactg cgtgcaacga tgggcctcga tccgggtctg cgtaccgggg taaaagtggc
    59581 ggtggtcgat gccactggca aactggtagc gaccgatacc atttacccgc acaccggaca
    59641 agccgcaaaa gcagcgatga ctgttgctgc gctgtgtgaa aagcataacg ttgaactggt
    59701 ggcgatcggc aacggtacag cttcccgcga aaccgaacgt ttctatctcg acgtgcagaa
    59761 gcagttcccg aaagtgaccg cacagaaagt gatcgtcagc gaagctggcg cgtcggttta
    59821 ctcggcttcc gagctggcag cgcaggagtt cccggatctc gacgtttcgc tgcgtggcgc
    59881 ggtttctatc gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa
    59941 atctatcggc gtaggtcagt atcagcatga cgtcagccag acgcaactgg cccgcaaact
    60001 ggatgcagta gtagaagact gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt
    60061 tccgctatta actcgcgtgg cgggcctgac gcgaatgatg gcgcaaaaca tcgtggcctg
    60121 gcgcgatgag aacggccagt tccagaaccg tcagcaactg ctgaaagtca gccgtctggg
    60181 gccgaaagcc ttcgagcagt gcgcgggctt cctgcgcatt aaccacggtg acaacccgct
    60241 ggacgcctct accgttcacc cggaagccta tccggtggtg gaacgcattc tggcagcaac
    60301 acagcaggca ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc
    60361 tgactttact gatgaaaaat tcggtgtgcc gacagtaact gacatcatca aagagctgga
    60421 aaaaccgggt cgcgatccgc gtccggaatt taaaaccgct cagtttgccg atggcgtcga
    60481 gacaatgaac gacctgcaac cgggtatgat cctcgaaggc gctgtcacca acgtcaccaa
    60541 ctttggcgcg tttgtcgata tcggcgtgca tcaggacggc ctggttcaca tctcttcatt
    60601 gtcgaacaag tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt
    60661 gaaagtgctg gaagtggatc ttcagcgtaa acgtatcgcc ctgactatgc gtctggacga
    60721 gcagcctggc gaaaccaact ctcgtcgcgg cggcggtaac gatcgcccgc aaaacaaccg
    60781 cccggcagcc aaaccacgcg gtcgtgaagc gcagcctgcc ggtaacagcg cgatgatgga
    60841 cgcgctggcg gcagcaatgg gcaaaaaacg ttaaacgccc ggactggccc ctaaacctgc
    60901 gcgataaggc gttcacgccg catctggcaa cgaacgcctt gcctgacata aaagtgccgg
    60961 agaatatctc cggcatattt attccacagc caaactcaca atatatttcg ttaatattta
    61021 tcacttcatt aacaactgaa accttaatta aacattagcc agtcctggta attcgctatt
    61081 cgaattatat tttcgctgcg atataacctt gagccacatc aacattgagt cagattatta
    61141 ttcaaaccaa cattcgcaca cattttaagt attgctgata gaaaccattc tcattatcat
    61201 tgtactattg attatttaat ctctccttcg ttggcaaatc atctggtctc atgtcgctgt
    61261 caaacgcccc atgaggtagt tatccagtta atgagaaaca agtaggcacc tatgcaatac
    61321 actccagata ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc ggcatatcgc
    61381 caaaaactgc tttctcttgg catgttacct ggctcctctt ttaatgtggt gcgcgtcgct
    61441 ccactcggcg accccattca tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa
    61501 gatctggcct tattagaagt ggaagcggtt tcctgttaat acggtgataa caacaatgaa
    61561 aaaattaacc ataggcttaa ttggtaatcc aaattctggc aagacaacct tatttaacca
    61621 gctcactggc gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga
    61681 agggcaattc tccaccaccg atcatcaggt cacgctggtg gacctgcccg gcacctattc
    61741 tctgaccacc atctcatcgc agacctcgct cgatgagcaa atcgcctgtc actacatttt
    61801 gagtggcgac gccgacctgc tgattaacgt ggtggatgcg tctaaccttg agcgtaacct
    61861 gtacctgacg ctacaactgc tggaactcgg cattccctgc atcgtggcac tgaacatgct
    61921 cgacattgcc gagaagcaaa atattcgtat tgaaattgat gctctgtcgg cgcgtcttgg
    61981 ctgcccagtg atcccgctgg tttcaacccg tggtcgcggt attgaagcgc tcaagctggc
    62041 gattgatcgc tataaagcta acgagaatgt ggaactggtg cattacgcac agccgctact
    62101 caacgaagca gattcactgg caaaagtgat gccttccgac atcccgctga aacaacgtcg
    62161 ctggctgggc ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg ccggtgaagc
    62221 gtcgcagcat cttgatgccg ccctcgcccg tctgcgtaat gagatggacg atccggcgct
    62281 gcacattgcc gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg taagcaacac
    62341 cctgacggca gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt
    62401 cctcggtctg ccgattttcc tctttgtgat gtacctgatg ttcctgctgg ctatcaacat
    62461 cggcggggcg ttacagccgc tgtttgacgt cggctccgtg gctctatttg tgcatggtat
    62521 tcaatggatt ggctacacgc tccacttccc ggactggctg actatcttcc tcgcccaggg
    62581 cctgggtggc ggtattaaca ccgtgctgcc actggtgccg cagatcggca tgatgtacct
    62641 gttcctctcc ttccttgagg actccggcta tatggcgcgt gcggcgtttg tgatggaccg
    62701 cctgatgcag gcgctgggct tgccggggaa atcctttgtg ccgctgatcg tcggtttcgg
    62761 ttgtaacgta ccgtcggtaa tgggtgcacg tacgcttgat gcaccgcgtg aacgtctgat
    62821 gaccatcatg atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt
    62881 cgcggctgcc ttcttcgggc agaacggtgc gctggcggtc ttctcgctgt atatgctcgg
    62941 tatcgtgatg gcggtgctga ctggcctgat gctcaagtac accatcatgc gcggtgaagc
    63001 gacgccgttt gtcatggagc tgccggtcta ccatgtacca cacgttaaaa gcctgattat
    63061 ccagacctgg cagcgtctga aaggcttcgt tctgcgtgct ggtaaagtga ttatcatcgt
    63121 cagcattttc ctgagcgctt tcaacagctt ctcgttgagc gggaaaatcg tcgataacat
    63181 caacgactcg gcgctggctt ccgtcagccg ggtgatcacc ccggtcttca agccgattgg
    63241 cgtgcatgaa gataactggc aggcaacagt tggcctgttt acgggtgcaa tggcgaaaga
    63301 agtggtggtg ggtacgctca acaccctcta caccgcagaa aatattcagg acgaagagtt
    63361 caatccggcg gaatttaacc tcggtgaaga gttgttcagc gcggtagatg aaacctggca
    63421 gagcctgaaa gacaccttca gccttagcgt actgatgaac cccattgaag ctagcaaagg
    63481 cgacggcgaa atgggtaccg gggcgatggg cgtgatggat cagaaattcg gtagcgcagc
    63541 tgccgcatac agctacctga ttttcgtcct gctgtatgta ccatgtatct cggtgatggg
    63601 agccatcgcc cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt gggggctgaa
    63661 tatcgcttac tcactggcaa cattgttcta tcaagtcgcc agctacagtc agcacccaac
    63721 ttacagcctg gtgtgcattc tggcggttat cctgtttaac atcgtggtta tcggtctgct
    63781 gcgccgcgcg cgtagccggg tggatatcga actgctggca acccgcaagt cggtaagtag
    63841 ttgctgcgca gccagcacca ccggtgattg ccattaatgg cttcacttat tcaggtgcgt
    63901 gatttgctgg cgttacgggg ccgtatggaa gcggcccaga taagccagac attgaacact
    63961 ccacagccaa tgattaacgc catgctgcaa caactggaaa gtatgggcaa agccgtgcgg
    64021 attcaggaag aacctgacgg ctgcctctcc ggcagttgta aaagctgccc ggaaggaaaa
    64081 gcctgtctgc gcgagtggtg ggcgctgcgt taaccatact ccatcgcctg ttttgaaaag
    64141 cccggtatgc gtttgcatcc gggctttttt gcgtgcggct ttccataaaa atgcaattct
    64201 tgcagcgcga cgtaagttcc tctgaaagcg tctcgcaggg atgaaaactg gctaatgcac
    64261 aggtgtggag tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga acgccatgag
    64321 caaaaagcag agttccaccc cacacgatgc gctgttcaaa ctctttttac gccaaccgga
    64381 gacggcacgc gattttcttg cgtttcattt accagcaccc attcacgcgc tctgtgatat
    64441 gaaaaccctc aagctggagt cgagcagctt tattgatgac gatctgcgtg aaagctattc
    64501 cgatgtgctg tggtcggtga aaacagaaca aggaccagga tacatctatt gtctgattga
    64561 acatcaaagc acctcaaaca aactgatcgc atttcgcatg atgcgttacg ctattgccgc
    64621 aatgcaaaat caccttgatg ccggatacaa aacgttgccg atggtggtgc cattgttgtt
    64681 ttaccacggt attgaaagcc cctatcccta ttcgctgtgt tggctggatt gtttcgccga
    64741 tcccaaactg gcaaggcagc tttatgcctc cgcatttccg ctgattgata tcaccgtcat
    64801 gcctgatgat gaaatcatgc agcaccgacg catgacgctg ctggagttaa ttcaaaaaca
    64861 tattcgtcaa cgcgacctga tggggctggt agagcaaatg gcctgcttat taagtagtgg
    64921 atacgctaat gacagacaaa tcaaagggct gtttaattac atactgcaaa ccggcgatgc
    64981 ggtacgtttt aacgatttta tcgacggcgt tgccgaacga tcaccgaaac acaaggagag
    65041 tttaatgact attgcggaaa gattgcggca ggagggggaa caatccaaag ccctgcatat
    65101 agccaaaata atgcttgaat ccggagtccc tcttgcagac atcatgcgct ttaccgggct
    65161 gtcagaagaa gagttggctg cggcgagtca gtaaagttct gtctcgccat tcaaaagtgc
    65221 ctacaccctc tgcttcaacg ccaccagcac gtgacgaaac tcgaccggat gcgaaataaa
    65281 cggcgcatga gccgctttgg cgaagatgta tgactcgctg tgtggccaga gtttatccag
    65341 catcggcacc actttgcgcg gcaccagacc atcgagatag ccatacagtc gcaaaaacgg
    65401 catgggtacg ttttgcagcg gcaagcggag atcaaccgtt ttgaggattt ccagcccacc
    65461 attgagcacg tcaacctccg gcatcggtaa cgccagaacg gttttcttca acgcccgcgc
    65521 atcctgacgc gccgtttcag tacccatggt ttgtaacgca aggaaccgtt ccaccgtacg
    65581 ctgaaaatca tcactgagtt gctgctgaaa tcccgccagc acgtccggtt ttatccccgg
    65641 ccactcgtca cgagcactaa aacaaggtga cgacgccacg gtgaccagcg cctgaacacg
    65701 ctcgggatgg gttaacgcaa tctggcttgc caccagcccg cccagactcc agcctaacca
    65761 aatggcttta tcaggtgcct gtcgcagcac ggcttcggcc atctccgcaa gtgacagcgc
    65821 accaaatccc cggctacgcc cgaagccggg caggtcaaca agatgcagcg taaaatgcga
    65881 gctaagttcc tcgtcaatgc aacgccacac ttcagcattc agtccccatc cgtgcagcag
    65941 cacaagatga acattcccct gacctttggt ctgccaccag atgttattca tccgctattg
    66001 ttctcttttg acttacaagg atgaacatat gctaacagta ccgggattat gctggctatg
    66061 ccgaatgcca ctggcattag gtcattgggg gatttgttcg gtctgctcac gcgccgcccg
    66121 tacagataaa acgttatgcc cacaatgtgg attacccgcc acacactccc atcttccctg
    66181 cggtcgctgc ctgcaaaaac cgccgccctg gcaaagactg gtcacggttg ccgactatgc
    66241 gccgccgtta agtctgctca tccaccagct taagttttcc cggcgcagcg aaatcgccag
    66301 cgccctgtca cgtctgttac tattggaagt cttacacgct cgtcgcacca ccgggttgca
    66361 attgccggat cgtatcatca gcgttccgtt atggcagcgg cgtcactggc gtcggggatt
    66421 taatcagagc gatttgctgt gtcagccgtt atcacgctgg ttgcactgcc aatgggatag
    66481 cgaagccatc acacgtacac gggccactgc gacccagcat tttcttagcg cccggctacg
    66541 caggcgcaac ctgaaaaatg cctttcgcct tgaattgcct gtgcaaggtc gccatatggt
    66601 gattgtggat gatgtcgtta ccaccggaag taccgtcgca gagattgcgc agttgctttt
    66661 acgcaatggt gcggcgactg tccaggtctg gtgcctttgt cgaaccttgt agagcctcga
    66721 tgatgggcgt attataacca actaaaatag tcaactatta ggccattact atgatccgta
    66781 tttccgatgc tgcacaagcg cactttgcca aactgctggc aaatcaggaa gaagggacac
    66841 aaatccgcgt atttgtgatt aaccctggca cgcctaacgc tgaatgtggt gtttcttatt
    66901 gtccgccgga tgccgtggaa gccaccgaca ccgccctgaa atttgacctg ctgaccgcgt
    66961 atgttgatga gttaagcgca ccatacctgg aagatgcaga gatcgatttt gttaccgacc
    67021 agttgggttc tcagctaaca ctgaaagcgc ctaacgccaa aatgcgtaaa gtggcagacg
    67081 atgcaccact gatggagcgc gtggagtata tgctgcagtc gcagatcaac ccacagcttg
    67141 ctggtcacgg tggccgcgtt tcactgatgg aaatcaccga agacggttac gccattctgc
    67201 aatttggcgg cggctgtaac ggttgttcca tggtcgatgt gacgctgaaa gaagggatcg
    67261 agaagcagct actaaacgaa ttcccggagc tgaaaggtgt gcgcgatctc accgaacacc
    67321 agcgcggcga acactcctac tactaagtta tcccctcttt tgtagattgc ccgatgcgac
    67381 gctaaagcgt catttcgggc ctacgttgaa catgtgccag acgttggcgc agcattgctt
    67441 tggcaaatcc ctcccttcgt ttccccgcga taatatgacc aacctctcat aatttaaatt
    67501 taccccgctc tggtgattct caaacgccag atgttacccg tatcattcac gtgggtacca
    67561 aacatactcc tgacatctga ctacaataat tagttttagt gggtatcagt cgtggtgccg
    67621 caatatctct gttcccgatt gggataatta gagcttgtcg tcagaaaatt gacgttaccc
    67681 ataacaaatg aaaggccagg taaatcatgc cattagtcat tgttgctatc ggtgtaatct
    67741 tgttattact cctgatgatc cgcttcaaaa tgaacggctt catcgctctc gtcctcgtgg
    67801 cgcttgcggt tggattaatg caagggatgc cgctggataa agttattggc tccatcaaag
    67861 ccggtgtcgg cgggacgctc ggtagccttg ccctgatcat gggttttggc gcaatgctgg
    67921 gcaaaatgct ggcagactgc ggtggcgcac aacgtatcgc caccacgctg attgccaaat
    67981 ttggtaaaaa acacatccag tgggcggtgg tactgaccgg ttttaccgtt ggttttgccc
    68041 tgttctatga agtgggcttt gtgctgatgc tgccgctggt gtttaccatc gcggcttctg
    68101 cgaatattcc actgctgtat gttggtgtac caatggcagc tgcactgtct gtgacccacg
    68161 gcttcctgcc accgcatccg ggtccgactg cgattgccac cattttcaat gccgatatgg
    68221 gtaaaaccct gctgtacggt actattctgg caatcccgac cgtgattctc gccggtccgg
    68281 tttacgctcg cgtgttgaaa ggtatcgata agccaattcc ggaaggtctc tacagcgcga
    68341 aaaccttcag cgaagaagag atgccgagct ttggcgtcag cgtctggacc tctctggtgc
    68401 cggtagtact gatggcgatg cgtgcgattg ccgaaatgat cctgccgaaa ggtcacgctt
    68461 tcctgccggt agcggagttc ctcggtgacc cggtaatggc tacgctgatt gccgtgctga
    68521 ttgcgatgtt cacctttggt ctgaaccgtg gtcgttcaat ggatcagatt aacgacacgc
    68581 tggtttcttc catcaaaatc attgcgatga tgctgttgat catcggtggt ggcggtgcgt
    68641 tcaagcaggt gctggtagac agcggcgtgg acaaatacat tgcttccatg atgcacgaaa
    68701 ccaacatttc tccgctgctg atggcctggt cgattgctgc cgtactgcgt atcgcgctgg
    68761 gttccgcaac cgttgcggca atcactgcgg gtggtatcgc ggcaccgctg attgcaacga
    68821 cgggtgttag cccggagctg atggttattg cggtgggttc cggtagtgtg attttctctc
    68881 atgtgaacga tccgggcttc tggctgttca aagagtactt taacctgact atcggcgaga
    68941 ccatcaaatc ctggtcgatg ctggaaacca ttatctcggt gtgcggtctg gtaggctgtc
    69001 tgctgctgaa tatggtgatt tgataacact gactgccgga tgcatcgcat ccggcatcaa
    69061 ccgcactcta cttcttcttc gctgcagctc tgcgccgtct gtccaaatcc ttcagcaact
    69121 tgttcacgcc atcatcggca aacatcgtct caagcgttgc ggaaagcttg cgtcgccagt
    69181 ttttatactg gtaactggtg ccaggaatat tcaccggttc ggccatatcc agccagtctt
    69241 ccggctgtag tcctaacaga gcactgttac tgtcggcaat gtagcgctgc aaaccacgat
    69301 taagcgtcgg cgtcatcgac atcaacgatg ccttatgccc ggcacgtttt ggcagacaac
    69361 catatttatg cagtgcatcc agcagccctt gcttcgccag ttcgcgatcc tgatacagac
    69421 cgcgcagtac cacttcatcc ggataccgcc ccagggtttt gcccagcgtt agatccccgc
    69481 tctcccagta gccgcgtagc gtaggcaggt catgtgtcgc cgcaaccgcc atcgactgct
    69541 ccggatacgc tttcggtgca cggaacgtct tctcgtggtc gttttcgaaa tagagcactt
    69601 tgtaggagta cacaccgctg ctgcgcagct taccgacaat ctctaccggt acggtaccga
    69661 gatcttcacc aatcaccata cagcgatgac gtttactttc gagtgccaga atcgagagca
    69721 gatcatccac cggatagtga acatacgcgc cctgatctgc cgtctcgcca tacggtatcc
    69781 accacaaacg cagcatcgac atcacatggt caattcgtaa tgcgccgcag ttttgcatat
    69841 tggcacgcaa cagctcgata aacggttcat aagcacgcgc ggtgatgata tgcgggtcca
    69901 ttggcggcaa tccccagttc tgccccaacg ggccgaggat atccggcggc gcgccaaccg
    69961 acgctttcag gcaatacagt tcacggtcgc accaggtttc cgccccacct tccgctacgc
    70021 caaccgccag atcacgatac aagccaatcg gcatttcata gccctggctt atctcccagc
    70081 aggcggcaaa ctggctgtaa gcctgccact gcaaccagag ataaaaatca acgtcatcgc
    70141 gatgttcttc gcagaactga cgcacttctg gtgaatccac gttctgatac atctctggcc
    70201 atgcaggcca gccccagcgc atttcgtcct ctttcacttg ctgggcatgt agcgcatcaa
    70261 aggctgcctg ccagaacagg ctgtcgcctt gatctgcaac aaactggcga aacgcggcca
    70321 tctgctcatc atcacgttgc gcgaaacctt tccacgccat tcgtaatgct gtcattttta
    70381 gggcggtaac cgtggagtaa tcgacccagt cggcatcccg cgcctgttgc agcgtctgtt
    70441 gcgtggtcgg caactgccac caggcctgag cctcttcgct aagatggaaa tcttcgacgg
    70501 cgttaacgtc gatataaatc acattcagcc agcggcgaga agacgggcta tacgggctgg
    70561 cgctctccgg atttgccgga tagagcgcat gaatcgggtt cagaccaatg aacgatccgc
    70621 cacgttttgc cacatccact agcatcgctt tgagatcgcc aaaatcccca ataccccagt
    70681 ttttttccga tcgcagcgta taaagctgga cgcaggcacc ccacagcttt tgtttattca
    70741 gcaacgcctg cggttcgtaa cagcgtttcg gggcgacaat caccctgcaa tgcccgcgct
    70801 ggtcttcctg ggtaagcgtc agcgtgtgat aaccttccgg cagcttcgtc ggtagattga
    70861 acgctttgcc ccccgttata tggcctttgt actgcgttcc ttcttcggtg gtcagcagcc
    70921 agctatattc gccgctgccc tccaccacca tcggcatttt tttgccgctg gtataaacca
    70981 tgacattcgg gactggcgtt accgccactt tcgtggcggt acgttgatgc atcgcgtcaa
    71041 gcaaacgccg tttggtttcg gcgctaatcg actgcggttt accgtgggca ttgatgtaat
    71101 gagggctaat ccccgccgcc agcgcggcat tatccagacg tttgctttcc atcgagcttc
    71161 cttagcgttt tgcctgccag atacgagcct gataatcgcg aatagagcga tccgagctaa
    71221 acataccgca gcgggcggta ttgaggatcg ccgcgcgagt ccaggcctcc tggtcgcggt
    71281 acagcacatc cacctgcttt tgtgcctcta cataggctgc gaaatccgcc atcaccagat
    71341 acggatcgcc gccctgtttg ccgatactgt gcagcatctg gtcgaaggca tgcttatcgc
    71401 cgtcgctgta tttaccgctt tccagctctt tcaatactgc gtccagcacc ttatctttct
    71461 tccgccattt caccgggtcg tagcctttgg ccagaattgc cttcacttgt tccacggtat
    71521 gaccaaaaat aaagatattt tcttcaccga ctttctcggc gatttcaacg ttcgccccat
    71581 ccagcgtacc gacagtaagc gcaccattga gcgccagttt catattgccg gtaccggaag
    71641 cttctttacc tgcagtcgaa atttgttcgg agatatccgc cgccgggatc agtttttccg
    71701 ccgccgaaac gcaataatcc ggcaggaaca ccaccttcaa cttatcgcca accagcggat
    71761 cgttgttgat cacgtcagcc actttgttga tcgcaaagat aatatttttc gccaggtagt
    71821 agcccggtgc cgctttcgcg ccgaagagga agacgcgcgg tacgcgatca gcctgtgggt
    71881 tttcacgaat ttctttgtac aacgccagaa tatgcagcag attcaggtgc tggcgtttgt
    71941 actcatgcag acgtttgatc tgaatatcga aaatcgcctg tggattgatc tcaataccgg
    72001 tacgaacttt cacaaactcc gccagacgga ctttattcgc ctgcttgatc tcgcgatatt
    72061 gctgacggaa tttcgcatca tcagcgaatt tttccagatt gatcagctga tcgagatcgt
    72121 tagcccactc ttttttcagt gatttatcca acagagccgc cagtgccggg ttgcactgtt
    72181 tgatccagcg acgtggggta ataccgttgg tgacgttatg gaatttgttc ggccatagct
    72241 ggtgatattc cgggaacaga tctttcacca ccagatccga gtgcagcgcc gcaacaccgt
    72301 tcaccgcgaa accgccaacc acacacaggt tcgccatatg cacttgtttg tcgtgcacca
    72361 ccgccagttt agcccacact ttttcatcgc ccggccaggt tttctccacc agcgttttaa
    72421 agcgagtatt aatttcgtta ataatctgca tgtggcgcgg cagtaagcct ttcaccagtt
    72481 tcacatccca gcgttccagc gcttctggca tcagggtatg gttggtgtaa gcgaaagttt
    72541 tgctggtaat ggcccaggcg tcatcccagc tcatctggtg ctcatcgatc agcacgcgca
    72601 acagttctgg aatcgcgata gttgggtggg tatcgttcag ctgaataact tcgtaatccg
    72661 ccagttcgtg cagtttacgc cccgccagat gatggcgacg caaaatatcc gctaccgaac
    72721 aggcacactg gaagtactgc tgcatcaagc gcagcttttt accggcagta tggttgtcgt
    72781 ttggatagag aactttggtc agtttttccg cattgatgcc ctgctgttcg gcacgcagga
    72841 aatcaccgtc gttaaattta gtcagatcaa acggatgcgc gtgcgtcgcc tgccacagac
    72901 gcagcggctg cgccacgcca ttacgatagc cgacaacggg gagatcccac gcttgaccgg
    72961 taatggtaaa ctccggctcc cagcgtccgt ctttcgtcac tttaccgcca atccctacct
    73021 gcacatccag tgcttcgttg tggcggaacc acgggtagtt actgcgatgc cagtcatccg
    73081 gcgcttcaac ctgtttgcca tccacaaaag actggcggaa caaaccatat tgatagttca
    73141 gaccgtaacc cgtcgcagac tgaccgacag ttgccattga atcgaggaag cacgccgcca
    73201 gacgtcccag accaccgtta cccagcgcag ggtcgatctc ttcttccagc aggtcagtca
    73261 gattgatgtc ataagccttc aacgaatcct gtacatcctg ataccagccg agattcaaca
    73321 ggttgttgcc cgtcagacga ccaatcaaaa actccattga gatgtagtta acatgtcgct
    73381 gattcgccac cggcttggcg aatggctgag cacgcagcat ttcggccagt gcttcactca
    73441 ctgccagcca ccactggcga ggagtcattt cagccgcaga atttaagcca taacgctgcc
    73501 actgacgtga aagcgcttcc tgaaattgct tatcgttaaa aataggttgt gacataggag
    73561 ttccactttt cttagatttt caacacaacg ttatcgctag tttgccaggc tcgatgttga
    73621 ccttcctcat cctgcggggg attaggcagg gaggagttgc ggggatgagc aaggaaatgt
    73681 gatctcgacc acttaaagct agtgcaaacc acaggattag cagcaaatca atgccataca
    73741 gcgcagaaaa tctgtatcta agtgcaaaaa atggccgttg cgtattttca aaaagcgaaa
    73801 ggtaactcta taaattaagt aaaggagtga aacagtttca caagtaaaat atccagtgtg
    73861 ctccatctca ttcttaatag atttattaag atcatctttt tagatagcac tttcatcagg
    73921 aatgaagaag aaacccttgc ttaaatgaat ctgatgaaca taagggaaac cagtattcac
    73981 gctggatcag cgtcgtttta ggtgagttgt taataaagat ttggaattgt gacacagtgc
    74041 aaattcagac acataaaaaa acgtcatcgc ttgcattaga aaggtttctg gccgacctta
    74101 taaccattaa ttacgaagcg caaaaaaaat aatatttcct cattttccac agtgaagtga
    74161 ttaactatgc tgattccgtc aaaattaagt cgtccggttc gactcgacca taccgtggtt
    74221 cgtgagcgcc tgctggctaa actttccggc gcgaacaact tccggctggc gctgatcaca
    74281 agtcctgcgg gctacggtaa gaccacgctc atttcccagt gggcggcagg caaaaacgat
    74341 atcggctggt actcgctgga cgaaggtgat aaccagcaag agcgtttcgc cagctatctc
    74401 attgccgccg tgcaacaggc aaccaacggt cactgtgcga tatgtgagac gatggcgcaa
    74461 aaacggcaat atgccagcct gacgtcactc ttcgcccagc ttttcattga gctggcggaa
    74521 tggcatagcc cactttatct ggtcatcgat gactatcatc tgatcactaa tccagtgatc
    74581 cacgagtcaa tgcgcttctt tattcgccat caaccagaaa atctcaccct ggtggtgttg
    74641 tcacgtaacc ttccgcaact gggcattgcc aatctgcgtg ttcgtgatca actgctggaa
    74701 attggcagtc agcaactggc atttacccat caggaagcga agcagttttt tgattgccgt
    74761 ctgtcatcgc cgattgaagc cgcagaaagc agtcggattt gcgatgacgt ttccggttgg
    74821 gcgacggcac tacagctaat cgccctctcc gcccggcaga atacccactc agcccataag
    74881 tcggcacgcc gcctggcggg aatcaatgcc agccatcttt cggattatct ggtcgatgag
    74941 gttttggata acgtcgatct cgcaacgcgc cattttctgt tgaaaagcgc cattttgcgc
    75001 tcaatgaacg atgcactcat cacccgtgtg accggcgaag aaaacgggca aatgcgcctc
    75061 gaagagattg agcgtcaggg gctgttttta cagcggatgg atgataccgg cgagtggttc
    75121 tgctatcacc cgctgtttgg taacttcctg cgccagcgct gccagtggga actggcggcg
    75181 gagctgccgg aaatccaccg tgccgccgca gaaagctgga tggcccaggg attccccagc
    75241 gaagcgattc atcatgcgct ggcggcaggc gatgcgctga tgctgcgcga tattctgctt
    75301 aatcacgcct ggagtttgtt caaccatagc gaactgtcgc tgctggaaga gtcgcttaag
    75361 gccctgccgt gggacagttt gctggaaaat ccgcagttgg tgttattgca ggcgtggctg
    75421 atgcaaagcc aacatcgcta cggcgaagtt aacaccctgc tggcccgtgc tgaacatgaa
    75481 atcaaggaca tcagagaagg caccatgcac gcagaattta acgctctgcg cgcccaggtg
    75541 gcgattaacg atggtaatcc ggatgaagcg gaacggctgg caaaactggc actggaagag
    75601 ctaccgccgg gctggttcta tagccgcatt gtggcaacct cggtgctggg tgaagtgctg
    75661 cactgcaaag gcgaattgac ccgctcactg gcgctaatgc agcaaaccga acagatggca
    75721 cgccagcacg acgtctggca ctacgcctta tggagtttaa ttcagcaaag tgaaattctg
    75781 tttgcccaag ggttcctgca aaccgcgtgg gaaacgcagg aaaaagcgtt ccagctgatc
    75841 aacgagcagc atctggaaca gttgccgatg catgagtttc tggtgcgcat ccgagcacaa
    75901 ctgttatggg cctgggcacg gctggatgaa gccgaagcgt cggcgcgtag cgggattgaa
    75961 gtcttatcgt cttatcagcc acagcaacag cttcagtgcc tggcaatgtt gattcaatgc
    76021 tcgctggccc gtggtgattt agataacgcc cgtagccagc tgaaccgtct ggaaaacctg
    76081 ctggggaatg gcaaatatca cagcgactgg atctctaacg ccaacaaagt ccgggtgatt
    76141 tactggcaaa tgaccggcga taaagccgcc gctgccaact ggttgcgtca tacggctaaa
    76201 ccggagtttg cgaacaacca cttcctgcaa ggtcaatggc gcaacattgc ccgcgcacaa
    76261 atcttgctgg gcgagtttga acctgcggaa attgtcctcg aagaactcaa tgaaaatgcc
    76321 cgcagtctgc ggttgatgag cgatctcaac cgtaacctgt tgctgctaaa tcaactgtac
    76381 tggcaggccg gacgtaaaag tgacgcccag cgcgtgttgc tggacgcatt aaaactggcg
    76441 aatcgcaccg gatttatcag tcattttgtc atcgaaggcg aagcgatggc gcaacaactg
    76501 cgccagctga ttcaactcaa tacgctgccg gaactggaac agcatcgcgc acagcgtatt
    76561 ctacgagaaa tcaatcaaca tcatcggcat aaattcgccc atttcgatga gaatttcgtt
    76621 gaacgtctgc taaatcatcc ggaagtgcca gaactgatcc gcaccagccc gttgacccaa
    76681 cgtgaatggc aggtcctggg gctgatttac tccggttaca gcaacgaaca aatagcgggt
    76741 gaactggaag tggcggcaac caccatcaag acgcatatcc gcaatctgta ccaaaaactc
    76801 ggcgtcgccc accgtcaggc tgccgtgcag cacgcgcaga aactgctgaa gatgatgggt
    76861 tatggggtgt gagtttgtat agcgttttta tgtacactct ttatactgta tataaaatcg
    76921 cactacaggc tcatgaatat gaaaccggaa agcaaagaag cgccaatcaa tatcagagcg
    76981 aaagcctcac agcgtgacct gattgatatg gcagcgaatc tggtcgcaaa atcgcgcact
    77041 gattttatgc tggatgccgc atgccgtgaa gcgcaagaca tcttattaga tcaacgacta
    77101 ttcatccttg atgacgagca atacgatgcg tttctcgctg ccctggacgc ccccatcact
    77161 gctgagcgtc aggccaaaat taacgcgttg atgaatcgta aatcgccatg ggaataacgg
    77221 ctcctactcc cctaacgtct gaacataatc tggctgactt ttgctgttcc gatcacggga
    77281 tgaatgaatg gctaaaaaag aaagcgttaa aaaatcacag ctccggtctg tctcgtgttt
    77341 acgttatctg tattgccaat acccgccagg taatcggcta ttactgcctt tctacaggca
    77401 gtattcaacg taatttggcc cctggagcca tgcgacgtaa tgctcccgaa tcgttgcctg
    77461 ttgtcgtatt aggcagactg gcaattgatc aagcctgggc aggtaaaggg ttgggcgtcg
    77521 cattgttgaa agatgcagtc tatcgcacaa tgtccatagc tcaacaggta ggggttcgtg
    77581 cgctcattgt tcatgcctta gacgattcag tacgcaattt ctatctgaag tatgcctttg
    77641 taccttcacc attccagtca cttaccttac tgtatcccat tactctggag taatttacct
    77701 ctaccggata gtcacactga tgagtctgat ccgggaggga tttcaatcag tgagtttggt
    77761 tatcatcacc cgcgttacgc catctgtttc agccagagta aaccgcaccg gcaagaaacg
    77821 ctccaccacg gcgatattgg tcagcagatg gcatgaggga tgggcgaccg taaattgtcc
    77881 cgcgcccgcc agcgccattg gcaacactag ctggtcagca agatattccc ccactgccgc
    77941 cgggcttgcc aggtagcgtt tcacctcttt caccaactgt gccgcaacca cctcggcact
    78001 gacgcgcttt tcaccgacga caaaaaagcg ttcggtgata ttttcacttt cgacttcaag
    78061 cgagacggta ttacccggcc cctggtcgcg cggcaggtta tgaatattct gctcatgcag
    78121 ggaaaaactt gccgccagcg tagcgatttc acgctcagcg acatgccgcg gcacgccagc
    78181 taataacacc tctccacgca tctgcacaat gttcccgcgc tcgccaagtt gcaaggtgtt
    78241 aaacgatgcc accggcgaga cttccgttgc caccacgccg cctccggcag gataaaaacc
    78301 gtgacgtaac agcgtggttt gctgatgaat tcctattttc gccagcagcg gctccaacac
    78361 ccggcggata aaatccgcag gcggggccga cgggttatcg gtaccgccgc tcacttcaac
    78421 acgcgaaggt ccatcggcaa accacagcgc gggcagcacc gtttgcagca ccagcgtaca
    78481 acttcctgcg ctaccgatag caaagcgata atcgccgcca cgcacggtgc cgggccggaa
    78541 gaccagacgc tgcgacccca gctccgcgcc ttccaccgtc gccccgcaaa tttccgtcgc
    78601 cgcctttacc gccgtcagat gctggcgcaa cagtcccggt tttgcccgcc cggcacgaat
    78661 gccggtgatg gtaaatggct ggccggttat catcgacaga ctcagcgccg agcgcatgat
    78721 ctgcccgccg ccttcgccct gtgcgccatc cagcgcaatc atccttttca tcatttatcc
    78781 ttttacgcac accacctgac gtagggtgta gataacttcc accagatcgc tttgtgccgc
    78841 catcaccgca tcaatatctt tatacgccat cgggatttcg tcgatcactt cggcatcttt
    78901 acggcattcc acatgcacgg tggcacgaat ttgatcttcc acgctgaaca tttttttcgc
    78961 tttagtacgg ctcataaccc gcccggcacc gtggctgcac gaacagaacg actcttcatt
    79021 tcccagccca cggacgataa agcttttcgc tcccatcgac cctggaataa ttccatattg
    79081 accagcacgc gcagacaccg cgcctttacg cgtcacgtag atctcttcac caaagtgctg
    79141 ttctttttgc acatagttgt ggtgacagtt gatctcttcc atcgccagcg tttgtggctg
    79201 tctgaccgtt ttctgcgtaa tgctctgcaa cgccgttact acgttttcca tcatcacatc
    79261 gcggttaagg ctggcaaaaa gctgcgccca ggccacggct ttcaggtaat catcaaagta
    79321 ttccgtacct tccataaagt acgccagatc acgcgacggc aatgtttcaa gcgtttcctg
    79381 catctctttt tgcgccagat cgataaagta agtcccgatg gcattaccaa ttccgcgtga
    79441 accggagtgc agcataatcc acacctggtc cgactcatca aggcagattt caataaagtg
    79501 gttacccgtt cccagcgttc ccaggtgttt atagttgtta gtgttaagga aacgcggata
    79561 tttttgcgtt aaccactgat aaccggcctc cagctcagcc catttcgcat cgacgttaac
    79621 aggcgggttt tcccatgcgc ctttatcacg tttacaacgg ccagtggtac gcccgtgcgg
    79681 caccgcagtt tcaatcgcct gacgcagctc cgccaggttt tccggcaggt cagccgccgt
    79741 taaagcggta cgtagtgcgt tcattccaca gccaatatcc acgcccaccg ccgccggaat
    79801 aatcgcccct ttggtcggga tcacgctacc aatggtggaa cctttaccca ggtgtacatc
    79861 aggcattacc gcaatatgtt tgaaaataaa cggcatcttc gccgtattaa taagttgctg
    79921 acgcgcatcg gcttctaccg gcacgccttt ggtccacatt tttaccgggg cattttcagt
    79981 ggtcagtaat tcgtaattca ttttgttttt ctcttttcgt tgtttgcggt cctgataatt
    80041 gcaaccgtcg tgccagaaaa ttaaataagc agattaattt tttaattcat tgttttaaaa
    80101 agattatatc tttacgtccg taaacggaga tttcccgcaa agccaattta ccgataatga
    80161 aatatcgtct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    80221 aacagtgctg gattatgccg ggcgcggcag tcagcgctgg tcgaaatggc gtccgtcact
    80281 ctgtatatgc cagcaagaat cgttagtcat cgatcgactg gaattattgc atgacgcccg
    80341 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaaccga
    80401 agtggtgagc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    80461 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    80521 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggctg aagcgcgtta
    80581 cctgcccgcc cggttgatac aatcttcacc tccgcgcaaa aaagaacagc cgcgcggcgc
    80641 aggtgaagtg acgattatcg atctcgattt aagcctttat aacgccatcg ccagccgctt
    80701 tgccgaggaa cgccagcaaa cgcttgattt tcttaagtcc ggtattgcca cgcgtaaccc
    80761 ccacttcaac cgcatgattg agcagattga aaaagtagcg attaaatccc gcgcaccgat
    80821 cctgctaaac ggcccaaccg gtgcgggcaa gtcatttctg gcgcgacgta tatttgaatt
    80881 aaaacaggcg cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg caaccctgcg
    80941 cggcgatacc gccatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcacg
    81001 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    81061 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gccattgaag aaaaaacctt
    81121 ttatccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    81181 gcgtgatttg cgccagctgg ttgccgatgg caaattccgc gaagatctat acgcgcggat
    81241 caatctctgg accttcaccc tgccggggct acgccagcgt caggaagata ttgaacctaa
    81301 cctggattat gaagtggagc gccacgcttc actcaccggc gacagcgtgc gttttaacac
    81361 cgaagcgcgg cgcgcctggc tggcctttgc gacatctccc caggcaacat ggcgcggtaa
    81421 ctttcgcgag ctttctgcca gcgtcacgcg aatggcaacc tttgcctcta gcggacgcat
    81481 tactctggac gtggttgaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    81541 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    81601 tatgcaactg gaacacgtta tcgcaatctg ccgccaggca aagtcgcttt ccgcagccgg
    81661 acgccagctt tttgacgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    81721 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    81781 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggtgtcggt
    81841 gtagacggca tctaccatgc tgatgctacc catattgacc atcgcgttac ggccaaattt
    81901 cgagtgatcg acaaccagca taacgtggcg cgagttctca ataatggcgc gtttggtgcg
    81961 aacttcgtgg taatcgaact ccagcagcga gccgtcgcta tcgatgccgc ttatccccag
    82021 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    82081 gccatcgcgg ctgcgtaatt cgccaccggc gagaataata cggaaatcct cttttaccat
    82141 cagcgtgtta gcaacgttaa gattgttggt gacaatgcgc aaattgctgt gattgagcag
    82201 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    82261 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    82321 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcaccgccat gatggcgcag
    82381 gatcaggttt tgctccgcca gctcattgag gtcgcggcga atagtctgcg ggctgacgga
    82441 gaagtgctcc accagctcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    82501 accgttgtga cgttgtgttt gtttcatttc taaatccctg gaattatttt cgttttcgcg
    82561 cattgagcga atcaacaaaa gccatcgcta aacccacggc taacccggcg atgtgtgctc
    82621 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    82681 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    82741 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    82801 atttttgctg cacatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    82861 cgctaccgag gcgtttttcc accgcaccgc cgagatacca ccaccagagc aggttaaaga
    82921 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    82981 tcagtgctgg atcgaatggc caagccagcc ataacatcac ttcctgatcg ccgagaattt
    83041 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    83101 gttcacgcaa ggcggcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    83161 ctgcctgcca gctcgccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    83221 gctccgcccg tacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    83281 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    83341 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    83401 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gacgttgcca
    83461 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    83521 cgccgcgcct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    83581 gttattacgc ataaaagcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    83641 tcccattgcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    83701 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    83761 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    83821 atgttataca tatcactcta aaatgttttt tcaatgttac ctaaagcgcg attctttgct
    83881 aatatgttcg ataacgaaca tttatgaact ttaacgaaag tgaatgaggg cagcatggaa
    83941 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    84001 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    84061 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    84121 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggcccc gcatatcgcc
    84181 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    84241 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    84301 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattcc
    84361 gactgttggg tagacgatgc ccgtctggtg ctcgccaacg cccagatggt ggtgcgtaaa
    84421 ggcggcgaag tgcttactcg tactcgtgct acttctgctc gtcgcgaaaa cggcctgtgg
    84481 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    84541 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg acgggatgca tctgccttcg
    84601 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    84661 aagcaggcct acattctgca aaacgaagat aaacgtattg tgttcgtgat cccgtggatg
    84721 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggcgatcc gaaagcagtg
    84781 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaaa
    84841 cagcttagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    84901 gagtccgact cgccgcaggc tattacccgt gattacaccc ttgatattca tgatgaaaat
    84961 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggcg
    85021 gaacatgcgc tggaaaaact aacgccgtat tatcagggta ttggcccggc atggacgaaa
    85081 gagagcgtgc taccgggtgg cgccatcgag ggcgaccgcg acgattatgc cgctcgctta
    85141 cgccgccgtt atccgttcct gaccgaatcg ctggcacgtc attacgcgcg tacttatggc
    85201 agcaacagcg agctgctgct cggcaatgcg ggagcggtaa gcgatcttgg ggaagatttc
    85261 ggtcatgagt tctacgaaac ggagctgaaa tatctggtcg accacgagtg ggtccgccgc
    85321 gccgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    85381 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    85441 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    85501 attgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatgt tgaatttgca
    85561 tgattttgta ggcctgataa ggcgttcacg ccgcatcagg catgaacaaa gcgccacgtt
    85621 gtcaacaatc tgacctactt tttgattcag ggaattaccg caaagcccac gccatcatac
    85681 ccaacgtaac aaaacttaag cacgttcccc actcactcca cttccgtatc tggcttctat
    85741 ttttgctacg ccactggcaa taatatccca acgcaaaacc aagctgtgca cttatcggac
    85801 cgacaaagtc gatccactgt tgagaccaaa atggcgaaaa attcagtggg agccagacaa
    85861 ctgtcagaaa gaaactgcgc atattttcca gcaaagcaat ttgcgctggc gcgacataaa
    85921 aaatgatatt ccacaggagc aaccagaaat aaactggtgc aaataccagc caacaatgca
    85981 gtctgctttt caattcaggt ggcgttctgc gcgcaaattg ccaaccaacg cccaccattg
    86041 cacagaagaa aagcgcatat tcagccgata accatagttg taattgcaga agggaaatac
    86101 gcctgtgcat gagcgggatt gagaacagta tttctcccag aaaactcagc gtgattacac
    86161 aggcagccag cgcaaccacc gtcggccaca ccaacaacgg tacaataaca aagccgagaa
    86221 ataacggata aatataaagt ctgattatat tcatcattat tgtcctgatg attcggggga
    86281 tgcttcatac agcggcacat accccacatc ctctaccaac tgttgcccct gctgacttat
    86341 aaaccagtca acaaatttct gcgtttccgg cgtgggattt tcacgcgtca ccatataggc
    86401 atccacaatg tatggataag tgccgttgcg gatattttcc tcggtaggcg caacgccatt
    86461 tatagccaaa agtttaatct gcttgcgcat tttgagcata ttgtcattca tacgagtgac
    86521 gtaatagtgg aaggtgtacc caatggaaga tggcgtgttt tgatagtcgg caacttcagt
    86581 aattaaaccg cccataagat caatcacagt ggatttttta gctggcagca ttggggtgtc
    86641 ttgcataacc aaccccttca taatcgtttg gcttccagaa tcttcagggc gctgccagac
    86701 ttttatatgt tcgtcactac cacctacttt attccagcgg ctagttttgc cgctaaaaat
    86761 atctttaacc tggtgctcag agagagaatt taccgggtta ttaatatcaa cgataaagac
    86821 aaaggcttcg cgggcgaaag cggtatattt cagtttaaca cccgcttctt ctgcacgttt
    86881 tttctgtcct tccgacggtt gtgcaacaaa aataaccgtc gcggaacctt taatcagact
    86941 atcgtaggct tcagctgttc gggtttgctg caaaccatac gcctgccagg ccagcgaggt
    87001 tcgatcctta tcttttggct cgggtactaa tttataatac gcggaggcat acagcgggta
    87061 taaggctgta gcgccgtaca ctctcggcca tcttgaccaa tcatcgctgt aacgtaatgc
    87121 aggttgcccc cgcagagacg tcagtccatg gctctcttct tccctgacgc tgttatagta
    87181 cccttcccgg tcgacacatc ccgccagtaa cgccgtgcct aatagtaaaa caccggtgaa
    87241 tttattcatc tcgctgattc ctttctctcg agcgttattc cattgttaga tacagcggca
    87301 catatcccac atcttctacc agactctgcc cctgcggcgt taaaaaccat tcgaccagtt
    87361 tttgtgtttc tgacgttgta ttttctctcg tcaccataaa tgcatcgacg atgtacgcat
    87421 atttgccgtt gcgaatgttt tccgccgtcg gtgtaatacc gttaatcgct agcaatctta
    87481 tatttttatc agcattcatt tgcgtcgcgt aatagcggaa ggtatagcct attgcgttgt
    87541 ttgtattacg gtattcggca acgactttaa tcattccctc catcacgctt gccacttccg
    87601 tttcctgcgg cgagatcatg cggacttttt tcatgacctg tgattgcatc actgtctggc
    87661 tgccagagtc ttccgggcgc tgccaggtct ggatctcctg atcgttaccg ccaacagtac
    87721 gccaattggt aattgcacca ctgaaaatgt cacgcacctg ttgttcagtc agggaattaa
    87781 ccggattatc cgcattgacg atgaaaacaa atgcttcacg ggcaaatggc gtgtatagca
    87841 aagtgacgcc cgattcctca gcgcgttttt tctgcccgcc ggagggttgc gccacaaaaa
    87901 taatatcggc atcaccttta acaatccggt tatatgcatc gggggtacga gagttctcca
    87961 gatattccca aacgtgaaaa tcctctggta ttacacttaa tgcataaaat gcagaagcat
    88021 aaattgggta cgcagccgtg gcgccatcga ttcgcggcca gttttgccta aactgaattt
    88081 gcggtttgcc acgcagcggc gtcagtcgat tattgagttt atcgggtcgc caggccctga
    88141 tatcaatatt ttcgcgtaca ccgacaccag gattttgctg cttatataaa tgggcctgat
    88201 acccggcgac aataccaagt aagagaagta gaaacaggag taagttacgg caacgtagtc
    88261 cggacgtgcc ggataaagga tatctggtcg gccaggcgta tcccagcgaa aaactgatat
    88321 gcgccccaac gggaataatg accatccacc agtactcacc acccactaat gagactaaaa
    88381 aaacgcccaa ccagggaagc ataaggtttg ccaaccaacc agctcccatc tggcgaaatt
    88441 gataatctgc cagatgtagc gtgattatcc agccaaccag ggttaatagc gcgggtaaga
    88501 gaaaaaagca ttgccgccag acggctattt ctctgttttc tggcgcgcta tcaccggccc
    88561 acacgcgacc agagaaaaat attattatcg cccccagcgt cagcataaag acgttgagta
    88621 gaagataaat tgttggcgtg ataaggatat tacaaacttc aataataccg gtaagcccga
    88681 cacccagcat ggcaacaacc gctgccaaaa attgcgccag tatggggatg ccgaaaaaag
    88741 tcattatcag cgaggtcaaa atccattttc tatttggcat cattctgtcc attcttcctg
    88801 aatgacggaa ttatactccc cgagaaccct cgccccctct ggacactttt ccgaaataaa
    88861 ggcgaaaaaa acgggacctt gtggccccgt tctgtttttt agcgaactta caatctcacc
    88921 ggatcgatat gccagatatg atcagcgtac tctttgatag tacggtcaga agagaagtag
    88981 cccatattgg caatgttcag catcgctttt gcggtccact cttcctgaag ctcgtagagt
    89041 tcatcgactt tatcctgaca atcgacatag ctgcgataat ccgccagtac ctggtagtga
    89101 tcgccgaagt tgatcagcga atcaaccaga tcgcgatagc gacccggatc ttccggactg
    89161 aatacaccgc tgccgatttg cgtcagcacc tgatgcagct cctcatcttt ctcgtagtat
    89221 tcacgcggtt tgtagccctg acgacgcagt tcttccactt cttccgctgt gttaccaaaa
    89281 ataaagatat tgtcagcacc gacatgatcc agcatctcga cattcgcacc gtccaacgta
    89341 ccgatagtca gcgcaccgtt aagcgcaaac ttcatgttac tagtgccgga agcttccgtc
    89401 cctgccagcg aaatctgttc agacagatct gccgccggaa tgatcaactg cgccaggcta
    89461 acgctgtagt tcgggatgaa cacgactttc agcttatcgc caatctgcgg atcgttgttg
    89521 atcactttcg ctacgtcatt gatcaaatgg ataatatgct tcgccatgta ataggccgaa
    89581 gccgccttac cgccaaaaat attcacgcgc ggtacccact tcgcatccag gtcggccttg
    89641 atgcggttat agcgggtaat cacatgcaac acattcatca attgacgttt gtattcgtga
    89701 atacgtttga tttgcacatc gaacaacgcc tttggattca ccaccacatt cagctgctgg
    89761 gcgatatact ccgccagacg ctttttattc tccagcttcg cctgatgcac agcgtgatta
    89821 accattggga aatcacagtg ttgttgcagc tcattaagca ggctaaggtc ggtgcgccag
    89881 ttacggccca ggtgttcgtc cagcacggct gaaagcgatg ggttcgctac cgccagccag
    89941 cgacgcggcg tcacaccgtt ggtgacgttg gtgaaacgac ccgggaagat tttcgcaaag
    90001 tcggcaaaca acgattgcac catcagatta gagtgcagtt ccgatacacc gttaactttg
    90061 tggctcacaa caaccgccag ccaggccata cgcacacgac gaccgttgga ttcatcaatg
    90121 atcgacgccc gtcccagcag atcggtatcg ttcggatact gttcctgcaa ggttttcagg
    90181 aaatagtcgt tgatttcaaa gatgatctgc aggtgacgcg gcagaatttt acccagcata
    90241 tcaaccggcc aggtttccag cgcctcgctc atcagcgtgt ggttagtgta ggagaagacc
    90301 tgacaacaca cctcaaacgc gtcgtcccag ctaaattggt gctcatcgat cagcagacgc
    90361 atcatctcag gaatcgacag taccggatgg gtatcattga gatgaatcgc gattttatcc
    90421 gccaggttat cgtaggtttt atgcaactga taatggcggc ttaaaatgtc ctgaatggtc
    90481 gaggaaacca ggaagtattc ctgacgcagg cgcagctcac gcccggagta ggtggagtca
    90541 tccggataca gtacgcgaga tacgttctcg gagtggtttt tatcttccac tgccgcgaag
    90601 tagtcaccct ggttgaattt accgaggtta atttcgctac tggcttgcgc actccacaaa
    90661 cgcagcgtgt tggtcgcgtc ggtgtcgtaa ccagggatta tctgatcgta agcgactccc
    90721 agaatctctt cggtttcaat ccagcgcgtt tttttacctt cctgctgaat gcgaccacca
    90781 aaacggactt tatagcgcgt gttgtggcgt ttgaattccc acgggttacc atattccagc
    90841 cagtagtcag gcgactcttt ctggctaccg ttaacgatgt tctgcttgaa cataccgtag
    90901 tcatagcgga tgccgtaacc gcgccccggc aaccctaacg tcgccagaga atcgaggaag
    90961 caagccgcca gacgtcccag gccaccgtta ccgaggcccg ggtcattttc ttcatcaatc
    91021 agctcttcga gatttaaccc catcgcttcc agtgcgccct gtacatcttc gtaaattcct
    91081 agcgacaaca tggcgttgga gagcgtacgg ccaatcaaaa actccatcga caggtagtaa
    91141 acctgacgag tttcttgcga caactgggca cggtttgaac gtaaccagcg ctccacgaga
    91201 cgatcgcgca cagcaaataa cgttgcgttc agccattcat gtttattggc gacgaccggg
    91261 tcctttccaa tcgtaaacat cagcttgtaa gcgatagagt gcttaagagc ttctacgcta
    91321 agcgtgggcg atgaatatgt aaacggagca ttcatatagg cgtttcctga aaactatttc
    91381 aagcgatagt aaagctcacg gtacgacttc gccgcgacct gccagctaaa atccattgcc
    91441 atagcctgac gttgcacaaa ccgccacaga gaaggacggg accacagtac aaaagcacgt
    91501 cgaatagccc gtaacagcga ccaggcatta ctatcttcaa agacaaaccc actggcgacg
    91561 ccatctgcaa ggttctcaag agaacagtca gaaaccgtat cagcaagccc accggtgcgc
    91621 cgcactaacg gcagcgtacc gtacttcaat ccataaagtt gcgttaagcc gcacggttca
    91681 aaacggctgg gcaccagaat gacgtccgca ccgcccataa tgcgatgcga aaatgcttcg
    91741 tgatagccaa tctgaacgcc cacctggccg gggtattccg ctgccgccgc aaggaaacct
    91801 tcctgcagca ccggatcgcc cgcgccgagt agcgccagct gcccgccctg ctccagaaga
    91861 cccggtaagg cctccagcac caggtcgagg cctttctggc tggtcagacg gctcaccact
    91921 gcaaaaagcg gcactttatc gtcaacctta agccccattg cgatttgtaa ctggcgctta
    91981 ttttccgctt tatcttccaa cgtatcgcgg gtgtaacgcg aggccaatag taagtccgtc
    92041 tctggactcc agattttctc gtccacgccg ttcagtacgc cggaaagacg cccttcacga
    92101 tgacgctgtt gcaacaaacc ttccataccg taggcaaact gcggttcggt gatctcgcga
    92161 gcgtaggttg gactgaccgc cgtaatgtga tcggcatagt acagaccggc cttcaggaaa
    92221 gagatttgtc cgttgaattc cagcccatga atattaaaga atgaccatgg caattggatg
    92281 tcattcatgt gatgtgcata aaacatgcct tgataggcca ggttgtgcac agtaaacacc
    92341 gacttcgccg gacgcccgcg cgccgccaga tacgcaggcg caaggcctgc atgccagtcg
    92401 tgcgcatgca ccacatcagg acgccagaat gggtcaagcc cgctggccat ttctgcccca
    92461 acccacccca gcagcgcaaa acgcaatacg ttgtcggtat aggcaaataa gttggtatcg
    92521 tgatacgggc ttcccggacg atcatagaga tgcggcgcgt caatcaggta tatgccaacc
    92581 ccgttgtaat gaccgaacaa cagcgtgata tgcccggcga aggtatcacg acgggatact
    92641 acctgcgcat cggtcacgcc acggcgaata tcgggaaatg caggcaacag tacgcgagcg
    92701 tcaacgccgt ctgcgatttg tgctgcgggt aatgccccaa taacatcagc cagaccgccg
    92761 gttttaagca gcgggaacat ctctgaacat acatgtaaaa cctgcattat cgctcctgtt
    92821 tatgccctaa cttccgtagc atttcgcgcg ttaccagcac gatgccttct tctgaacgat
    92881 agaaacgacg tgcatcttcc tctgcgtttt caccaatcac catgccttcc ggaataacac
    92941 aagcacgatc gatgacgcag cggcgcagac ggcacgagcg acctacccat acttccggta
    93001 acaatacggc ggaatcaatg ttgcagaatg aattcacgcg aacgcgcgag aacagaacgg
    93061 actgcaccac caccgaaccg gagatcacac aaccgccgga aaccagtgag ttaagggtca
    93121 tcccgtggct accggagcga tcctgcacga atttcgctgg cggtaatgat tcattgtagg
    93181 tgcgaattgg ccaattgcga tcgtacatat ccagttccgg caccacagag gccagatcga
    93241 ggttcgcttt ccagtaagct tccagcgtac ccacatcgcg ccagtacggc tcggcatccg
    93301 gatcggattg tacgcaagag agcgggaacg ggtgcgcata ggccagaccg gcttcggtga
    93361 tcttgggaat caaatctttg ccaaagtcgt ggctggagtt ctcatcgcga tcgtcttctt
    93421 ccagcagttc atacagatag tcggcgtcaa agacgtagat acccatactc gccagagatt
    93481 tgctcggatc gttcggcatt gacggcgggt tagcaggttt ttcaacgaat tcgataattt
    93541 tatcgttctc atcaaccgcc ataacgccaa atgcggaggc ttcttcaatc ggtactggca
    93601 tacaagcaac ggtgcaacgt gcgccttttt cgacgtgatc gataagcata cgcgagtagt
    93661 cttgcttgta gatatggtcg cccgccagga tcaccacgta ttccgcttta taacggcgaa
    93721 taatatcgag gttttgggtg accgcatctg cggtgccgcg ataccagttt tcccctttca
    93781 ttctctgctg tgctggcagc agatcgacaa actcgttcat ttcttcattg aagaatgacc
    93841 agccgcgctg aatgtgctgc accagagtgt gggactggta ctgggtgatc acgcccatac
    93901 gacggatccc ggagttgatg cagttagaca gcgcaaagtc gataatgcgg aacttaccgc
    93961 cgaagtgtac ggccggtttt gctcgcttat tggttaaatc cttcaggcgg gtaccacgtc
    94021 ctcccgccag tatcagggca acagatttca atggcagctg gcgcgccaac attaagtgat
    94081 cgttcttctc taaactaacc atgactaact ccttttttat catctctgga acacacacaa
    94141 tccgtgtgca ggtccctgcc agacagccgt aatcactggg ttatcctctc cagcgaatgg
    94201 gggaatggcg tgccactccc cagcaggtaa aacaatctct gttacctcaa gcgtggcgtt
    94261 aattgcgatc aaaaagcgat ccgagagcag aatttgcagc tgtttcggcc cgttttgcca
    94321 ctcatccgtg cttaaaggtt gagcatatcg atttagccaa cggacattgc cgtcgccttc
    94381 ttcccaccag cgattctcca ccaaagcggg aatacgcttg cgcagatgga ttaacgcggc
    94441 ggtaaatgcg gttaaaccac tgcttgcctg cgaccagtcc aaccaggtta attggttatc
    94501 ctgacagtag gcattgttat tgccatgctg gctgtgacca tgttcgtcac cggccagtaa
    94561 catcggcgta ccctgggaga gcaacaacgt tgttaacagg gcgtgaatgc tgtcgcgccg
    94621 ccgttcaacc aggtcaaggg taccgcctac cccttcttta ccatgattgt tactgtaatt
    94681 gttgttggtc ccgtcgcgat tttcttctcc gtttgcttca ttgtgtttat ggttgaaaca
    94741 aacgcagtcg cgaagcgtaa aaccgtcatg cgcggtgacg agattaatcg cggcactcgg
    94801 cagacgacca ttacgtttaa aaacatcgct ggaggcagca aaacgcccgg caaacgcccc
    94861 cagaggcaaa tcataatgta gccagaaacg acgggcagca tcgcggaaat gatcgttcca
    94921 ctcggcaaac agcggcggga aatttcccac ctgataacca ccaggagcga tatcccacgg
    94981 ttcagcaatt aacttcacct gcgagagcac cgggcagttc tggatagcgg taaacaacgg
    95041 cgcatcctga cggaactctg gcgtacggcc catgactgcc gccagatcaa agcggaaacc
    95101 atcgacgtgg caggtttcta cccaataacg caggcaggcg ctggcataat ccaccaccgc
    95161 cgggtgactc aaattgagcg tgttgccaca accggtccag ttgtgataat cgccgtcttc
    95221 tcttatccaa taatagctac ggttgtcgat cccacgcagc gagaataacg ggccgtcgag
    95281 gtccagttcc gcactatggt tgagcacgat atcaagaatg acttcgatac ccgctttatg
    95341 cagtgctttg attgcatcgc gaaactcatg cagcgccgtt tctggcgagc aggcatacgc
    95401 cggatgcagc gcaaacatcg ccaccgggtt gtaaccccag tagttactta gccccatgcg
    95461 ttgcagacgt ggttcactgg caaactgcgc cactggcagc agttccagcg cggtaatgcc
    95521 caattgtttc aaatagttga tcatcaccgg atgcccgagg gctttataag tgccacggat
    95581 ctcgaccggg atctccgggt gcaggtacgt taatccttta acatgggctt cataaatgat
    95641 ggtgctgccc cacggcgtgc gcggcggggc atcatcttcc cagtcatagt gatcaaccac
    95701 cactacgcat ttcggcgcaa tggcggcgtt gtcgcgatag tcaggttcat tatgaccggc
    95761 gtgcagcagc gggttatctt taaactcccc gtcaatttgc cgcgcgcaag gatcaatcaa
    95821 caacttcgcc gggttaaagc gatgcccctc ggcgggttgc caggggccat gaacgcgata
    95881 accataacgc aaacccgggc gcgcatccgg cagataaccg tgccaaatgt cgccactgtg
    95941 ccctggcaag tcatagcgat gttcctggcc attggcgtca aagacacaca gttctacccg
    96001 ctcggcatga gcggagaaaa gtgtgaagtt gacgccctga ccgtcgtaat gcgcgccgag
    96061 gggagcgggt ttgccaatgg cgagttgtgt cattctgcct cccgaaccag ccagatagtg
    96121 gccagcggtg gtagcgtcag gcttagtgaa tgctgacgac cgtggctggc aatctcatcg
    96181 ctgtgtaccg tgccgccatt acctgcatta ctgccgtgat agtgcatgga atcggtattg
    96241 aggatctcac gccatttgcc tggctgatta atgccgaagc gataatcatg acgcggcacc
    96301 ggcgtaaagt tactggcaac gatgatttcg ttaccctctt tatcgcgacg cacaaagatc
    96361 agcaccgagc gttctttgtc atccaccacc agccattcaa agccgtacgg gtcaaaatcc
    96421 agttcatgca tcgctttatg gtggcggtag gtgaggttca gatcgcgcac cagacgctgg
    96481 acaccgtggt gccagttatc gccgccttcc aacagatgcc agtcgaggct ggcgtcatgg
    96541 ttccactcgc ggccctgggc aaattcgttc cccatgaaca gcagtttctt gcccggaaat
    96601 gcccacatcc agccatagta agcgcgcagg ttagcgaatt tctgccatgc gtcgcccggc
    96661 atgcggtcga gaatcgattt tttaccgtgg accacttcat catgcgacaa cggcaggacg
    96721 aagttttcag tgtagttgta gagcatcccg aaggtcagtt tatcgtgatg atactgacga
    96781 taaatcgggt cgagcttcat gtagtccagg gtgtcatgca tccagccgag gttccacttg
    96841 taccagaagc ccagaccgcc catatcctgc ggacgagaaa cgccagggaa atcggtagac
    96901 tcctcagcca ttgtcaccgc accggaaacc tgctcaccaa gaatacggtt ggtattacgc
    96961 aagaattcaa tcgcttcaag attctcgcgc ccgccaaatt cgttcgggat ccactccccc
    97021 tctttacggc tgtagtcacg ataaatcatc gacgccaccg catcgacgcg cagcgcatca
    97081 ataccaaaac gttcaatcca gtaaagcgcg ttaccgacga ggaagttact gacttcacgg
    97141 cgaccatagt tgtaaatcag cgtgttccag tcctgatgat agccttcgcg cggatcgctg
    97201 tgttcataca agttcgtacc atcaaattcg gcaagcgcaa agtcatcagt cgggaagtgg
    97261 cctggcaccc agtcgagaat cacgttcaga ccagctgcgt gtgcagcatc aatgaaataa
    97321 cggaagtcgt cgcgagtacc aaaacggcgg gttggtgcat acaggccggt tggctgataa
    97381 ccccaactgc catcgaaggg atgctcgtta aggggcagta gttcgaggtg ggtaaagccc
    97441 atccatttag cataaggcac cagttgatcg gccagctcgc ggtagctcaa ccagaaattg
    97501 ttgtcggtgt ggcgacgcca ggagcccagg tgaacttcat aaatagagat tggcgcatca
    97561 aactgattcg cttttttgcg ctcttcagtc tgtacaacct tttccggcag cccgcaaata
    97621 agagacgcgg tttccgggcg catttgcgct tcgaaggcat aagggtcgga cttcagacgc
    97681 aagttgccat tggcatcaat catctcgtat ttatagagct gaccgttatg cgccccaggg
    97741 ataaacagtt cccagatgcc gctctcttta cgcaggcgca tcgggtgacg gcgaccgtcc
    97801 cagtagttga attgcccaac caccgagacc cgacgggcgt ttggagccca gacagagaaa
    97861 cgcgtacctg tgacgccatc catagtatct gcatgcgcgc ctaaagtttc atacgggcgc
    97921 aggtgagtac cttcagataa tagccaggca tccatttcct ggattagcgg accaaaacgg
    97981 taaggatcat caatcaggtt ttgctgccca tgccagacaa cagccaactg atagcggaaa
    98041 aaattcttac gtcgcggaat gacgccacta aagaatcccc gtgagtcgag acactccagt
    98101 tttgcgagtt tgcgcccggt tttcggttca atcacccaca catcggtagc gtcgggtaaa
    98161 agggcacgga cttccagtcc cgcggtggtt ttatgcattc ccagtacgga aaaaggatcc
    98221 gcaaaatggc ctgcaattag cgcgttaatc acgtctctat cgatacgatc ggacatgctt
    98281 gtcttcctgt tttattgtgt caccccatcc aattggattt ttgacttctg gttgtgacat
    98341 ttttttgacc tgaacggcgc agcactctgt gcatcctctc tgcgtcgtcc tcacttcagg
    98401 taaggctgtg aatactcatg tattcagcca cccttaaaga atagccaatg ctctatttaa
    98461 ctcccggtaa atcatgaaac atctgcgctt actcctgtat tacgcactaa caggggcggc
    98521 atcgcgcccc agatttaatg aataaagatt acgccagttg acgaagcatc cgacgcagcg
    98581 gctccgcggc cccccacagc agctggtcgc ccacggtaaa ggctgacagg aactctggtc
    98641 ccatattcag cttacgcagg cggcctaccg gcgtggtcag cgtgccggta acggcagctg
    98701 gggttagctc acgcatagtg atttcccgat cgttcggaac gactttcgcc cacggattgt
    98761 gcgcagccag cagttcttcc acggtcggaa tcgacacatc ttttttcaat ttaatagtga
    98821 atgcctggct gtggcagcgc aatgccccga cacgcacaca taaaccatct accggaatta
    98881 cggaagatgt gttgaggatc ttgttggttt ccgcctgccc tttccactct tcgcggctct
    98941 gaccgttatc gagctgtttg tcgatccacg gaatcaggct acccgccagc ggcacgccaa
    99001 agttatccac cggcagctca ccgctacggg ttaaggttgt gactttgcgt tcgatatcga
    99061 gaatagcaga ggacggggtc gcgagttcat ctgctacatg gccatacaga tggcccatct
    99121 gggttaataa ctcacgcata tgccgcgcac caccgccgga agcggcctgg taggttgcaa
    99181 cggacaccca atcaacaaga tcattggcga ataaaccacc caacgacatc aacatcaggc
    99241 ttacggtaca gttaccgcca acaaaagtcc tgatgccatt atttaatccg tcggtaatga
    99301 cgtcctgatt gacggggtca agaatgatga tggcgtcatc tttcatgcgc agagacgatg
    99361 ctgcgtcaat ccagtaaccc tgccatccgc tttcacgaag ctttggatag atttcgttgg
    99421 tataatcgcc gccctgacag gtcacaatga tatcgagggc ctttagcgcc tccagatcaa
    99481 aggcatcctg aagtgtgcca gtggttccgc caaaagacgg cgcagcctga ccaagctgag
    99541 aagtagaaaa gaagacaggg cgaatggcgt cgaagtcgcg ctcttcaacc atgcgttgca
    99601 tgagaacgga gccgaccata ccgcgccagc cgataaaacc aacatttttc ataagcgttt
    99661 tttcctgcaa agatgtgtgc tgtataaatg tgccggtctc ctcttggcac atctttcacc
    99721 atacaaaaag cagccaaagt cgcaagtgaa attaatcaat gatagcgaag ccatcagtaa
    99781 tgcgacttat cctgctttgt tagcacgcag aaagtccgcg gcaattatca gggaatttga
    99841 gttatgaatg aaatcatttc tgcagcagtt ttattgatcc tgattatgga tccgctcgga
    99901 aacctaccta ttttcatgtc cgtactgaaa catactgaac cgaaaagacg gcgggcaatc
    99961 atggtgcgag agttgcttat tgctctcctg gtgatgctgg tgttcctgtt tgcgggtgag
   100021 aaaattctgg catttctcag cctgcgagca gaaaccgtct ccatttctgg cggcatcatt
   100081 ctgtttctaa ttgccattaa gatgattttc cccagcgctt caggaaatag cagcgggctt
   100141 ccggcaggtg aagagccatt tatcgtgccg ctggcaattc cgctggtcgc cgggccaact
   100201 attctcgcca cgctgatgtt gttgtctcat cagtacccga atcagatggg acatctggtg
   100261 attgctctgc tgctggcctg gggcggcacc tttgtcatcc tgctacagtc ttcgctattt
   100321 ttacgtctgc tgggcgagaa aggggtgaac gcacttgaac gcctgatggg attgattctg
   100381 gtgatgatgg caacccagat gttcctcgac ggcattcgaa tgtggatgaa ggggtaaggt
   100441 tcaaacaggt gctgcacaca accagacgcg gcacctgttt tattacctga actgataatg
   100501 accttcgccc aatcggataa cccttccctc ttcttctgcg cggttcagcc acacactaat
   100561 ttgttcgatg gttaaaccag tacaagacgc cagcctttca atacttatcg attcctttgc
   100621 aaggatcgcc agttccgcca caaagagttg ataaaaatcg acaggcgtat acgcgggcat
   100681 tactgcgtaa tttgcgtcat cttcatgcag cgaaaacagt tctggttgag cgttacggag
   100741 tgctctgtca tacatcagcc caggaacgtc tggcagtctg atcgtcacat tctcagcctg
   100801 ttcagcactc catgacgccc cattttctac taatcgtgaa ttggctgatt gcatatcctg
   100861 atttggttta acctgcacag gcagccactg atgttttaat gcctccatcg ccccggttat
   100921 cgttccaccg gtcattcccg cacgtacaac cattgcgctt tcagcaaggc aataaatata
   100981 tttatttcgc gccatcgcat tgccgacggt gaaacgtacc tctgggtaaa acggcgaaat
   101041 caacaccagg ttgcctgcta taagcccttc acgccatttc actaacgtac tcgtttttaa
   101101 caagctatca gctaatacgc caacggcagt tcccccggcc tccagtgccg acgccattgc
   101161 acattcatca atacctcgcg caccaccaga gataacgcaa atcccctgtt gggccagttt
   101221 cgcggccagt tgttgggtat agcgcaaatc gtcagtcgga gcatctcgcg agccaacaat
   101281 cgccatacct tccgcttgca gtaatgcttt attcccgcag ccaaataaaa cgggaggtgc
   101341 atccgttcgc aatcggtttt tcaagcgaac gggataatca gcatctccgc gggttaagat
   101401 ccataaaccg gcgcgttgcc acttatctac cgccaacgcc agactatgac cacgcgccag
   101461 caagccaaga atacgctcct gagaaatacg cggatcgctc cagcctgtaa gtttctcttg
   101521 cggttgcggc accagaagct cggcgggact gatacgttgg tgcttcagcc ataatgcaaa
   101581 cttcccccat tcactattac taagaggttt atacttactc gccgccgcgc gagaaaaatc
   101641 gctggttagc aggagagttg cttgtgcatt ggctgaaaga ttcatcaatt ttttaccgag
   101701 gtagaagcaa gggcaagagg ataaaccgtc gggcaacctg cctggcgtaa cagtgctgtt
   101761 agaactgtca gcgtccatgc agaatccacg atatcgtcaa ccagcaacgc cggaccaggc
   101821 atcaaaggag gggtaatcac aaacgccccg tcgagatttt gacactggtg gaaacggttt
   101881 tgctgcattt tctgcggtgg attgtccacg actttttcaa tggcatcaat aaaaggtaag
   101941 ccaagtttcg ccgccagtcg ccgggcaaaa tcaggaacca ggtcgaggtg ctttcaaaat
   102001 caggaaccag gtcgaggtgc tttaatgaag gaacgcagca tacccaggtt ggttcaggat
   102061 gcggattcca gcgttggcga accatttccg cacatgcctc caccagttca tcactgaatc
   102121 gccccgtttt tttctcctgt gctacctgct ttccccaccc ggaatatccc caacgagaaa
   102181 ggattcgccc cgtagatcct tgtaaactcg caggcaagtt ccctttaaat ccgtattgcg
   102241 taaaagcccc agctgccacc tgtttattga gatttaatga caggtcagcg tgtttaataa
   102301 ataagtttgc cgcatggagt aagccgctat caatgtcggg cgacaacaac ggatgctgaa
   102361 gacaactgct acatttacca caatgttcgg cacctggctc atcgagtgcg cggcgcagaa
   102421 attgcatttt gcactccttg gtcgtgatat agctttcaac gtctgccagc tcactttttc
   102481 tctgattcat caaatggtta attcgttcat ggtcaggaga aaaactgaca attgttctgc
   102541 gccataattt ctcggtatac accacaggcg atggattttc agctaccaat aatttcagtg
   102601 ctttttctat ttgcccataa cggagattcg tccgttgttc aatgcctcgt aaggtaagac
   102661 cgtcattcac gctaagtacg ttgagtattt catgaatttg cgcctccgca ggaaaggcgc
   102721 tttcacggaa gaatttatga atagcacggt cttcactgcc gcaaagcagt atgccaacag
   102781 cagaatcgat accgcgaccg gcacgcccca cctgttagta gtaaccaact atcgaacctg
   102841 gcatctggta atgaataaca aaccccaaat ccggtttgtc aaagcccatt cccagcgccg
   102901 ttgtcgcaac cagcacttta attttatttg ccagcaacgc ctgctccaga tattccctgg
   102961 cagtattaga atcttccgcg ccttcacaag tcacgccgct gtagtaagca aatgcactga
   103021 taccattttt ccttaaccac tcggcgacaa gttcggcatc gcgagtagtc agggtataaa
   103081 caattcccga tttggaaaac tgagggataa ccgttgctaa ccatgccaga cgggatgagt
   103141 gttcacctaa gactaaggca tcaagcgcca aactttcgcg agccagcgtt ccacgctgaa
   103201 tcacaatgtc acccaattgc tgacggatat cctcaacgac acggttattc gctgtcgcgg
   103261 ttgtacccag aacaggggta ttcgcaggta gttgcgcaaa atatctaata tacgtcggta
   103321 atccgggcga aaatcgtggc cccaatcaga gatgcagtgc gcctcgtcga ccacaagcaa
   103381 gccaatatga tcggctatag gatataaaac cgtttcgata aagtcctggt ttgccaaacg
   103441 ttccggggaa accagcaagc aatcgacctc accttgcagc aacttatcgc taatgcgctg
   103501 ccattcttct ctgtttgtag aattcaacgt ttcagccgta atacctaacc gttcagcggc
   103561 ggcaacctgg ttacgcatca gggcaagcaa aggagagatg ataattgtcg ggccagcgcc
   103621 gcgatcgcgg aagattttac tggcgatgaa gtacacagca cttttccccc agccagtgcg
   103681 ttggacaacc agcagttttc gccgttggtt caccaccgcg tctatggctt cccattgccc
   103741 ttccctgaaa gtagcggtgg tatttcctaa catccgctgt aaaagcaatt cagctgcgtg
   103801 cttttccatc tttatctcct gcgatcttta ggcagtgatt agaacggatt atttgcaggt
   103861 aaatttgcga gagagatcaa aaagatgaag agaggagaaa agagaaatga attactgcga
   103921 gcaagctcgc agtaatttgt cgcagtaatt aactcaacag ctggaacgca atcatcccaa
   103981 cgatggcacc gacagtgccg aggatggttt ccatcatggt ccaggttttc agcgtttcgg
   104041 cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta acgtggctga
   104101 caacaatcga accaccggcg atacaaatcg acagcgccgc catttgcgca ccggagtagt
   104161 tcagttgttc aataaccggc atcaccagtc ccaccgccgt taaacaggca acggttgcag
   104221 aaccctgaat gatgcgcact gcagcggcca gcacgaagca ggtgatggca atcggcaggc
   104281 ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc agcacctgtt
   104341 tgaacacgcc gcccgcacca atcaccagca gaatgatccc cgccggttgc agcgcgtgac
   104401 cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg ccataaatcg
   104461 ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg aaccattcgt
   104521 aagcggtaga gccttccggc acaaaacgcg cggcaatggt tttcagccct accagcacca
   104581 gcggcagcag gatcagcgac aggctgaatc cgaaggatgg cattttgcct tcgccgagat
   104641 gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg aagttacccc
   104701 acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc aggatcatcc
   104761 agccaaaatc ggcgttcatc tgcgatgcca gcagcattgg cgctggccct ggcaccagga
   104821 acgccgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc aggttcgtac
   104881 cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc acttcaaaga
   104941 acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg cggctgtgac
   105001 cgaaggattt aagcattttg acggcaatct gatcgactgc gccggtttca tgtaagatct
   105061 tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg cctcccatcc
   105121 ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg ccagccccca
   105181 tggacaccac cattaaagcc aggaaagcgt gcatacgcgc cttcatgact aaaaacagca
   105241 gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact acttatttgc
   105301 cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga tcgatatcca
   105361 ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac tgcgtcacca
   105421 acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcggctttca atcacatcaa
   105481 aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc aagtcacgat
   105541 agtgtttttt caatgcagaa cagacgatca gcgacacttt attagtgcgc tgcatagcaa
   105601 acgcggcgtc gttcagcgcc tgcaaccacg gtttgcgatc gtcgtcattc agcggttcgc
   105661 cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca agaaacgcgg
   105721 catgaagttg atgcgccact tcacttgcga ccgcagattt gccgctgccc gatacgccca
   105781 tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc ccacaataca
   105841 agaattaaaa atgttacggg taactgttat cggtaacatt gtccagccgg acaatatcag
   105901 aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat ttgtgggctt
   105961 aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt gtcacagatt
   106021 cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc tcacgcggcg
   106081 tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg tggaaaccgg
   106141 caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac gccgcgccga
   106201 ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc cgccgtgcct
   106261 ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc acgctatatg
   106321 gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg atagtacgtt
   106381 cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc gcgagcaata atggctgtgg
   106441 tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgaga cacggcgact
   106501 ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt aaggtgcgcg
   106561 gcgtgtgggt acgttcggtg aggatcagtc cgtcgatatt ccatgagagc attgattcga
   106621 gacgttcttg ctccatttcc ggtttataac cgtagtgggc cagcatggtc tgataaccgt
   106681 gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg ttggtgagag
   106741 aaggtaacag gacgccaatc gcccggctgg tggcgttaga gaggatatcg ggcgcacgat
   106801 tgggaatata gcccagttca tcaagagccg cggcaatctt gccgcgtaga gcgacggaaa
   106861 cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct acacggtcag
   106921 ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa aagagataga
   106981 ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg cttgtttaca
   107041 attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgct gcaaaacccc
   107101 ggaagcgtac atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa ggcaggcttc
   107161 aaagatgccg ttgagcttta aaccggcggc agatcgaaca gtaacacttc gctgtcgcta
   107221 tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct gatcgaggct
   107281 ttcacgccgt taatggtgac attgcctttc accacctgga tccagacgcg gcgttcagcg
   107341 gcaatctgat gcaccgactg ctcatctttc agcaacgccc agcggtacag ttccatatcc
   107401 tgatgcactt tcaatgaacc atcacgcgca tctggcgaaa gcaccagttg tttgccctgc
   107461 actgcatcga agcgacgctg ttcataacgc ggcgtaatgc cgttttcttc cggcatgatc
   107521 cagatctgat acaggtgcag acgctcggtg ctgcttgggt tgtactctga gtgacgaata
   107581 cccgtacccg cactcataat ctggaactcg cccgccggaa cctgctcttt attgcccatg
   107641 ctgtcctggt gctcaacagt accttccagc acgtaggtca aaatttccat atctttatgc
   107701 gggtgagtgc cgaagccctg ccctgcttca atcacgtcgt cgttaatcac gcgcagcgcg
   107761 gagaagccca taaagttcgg atcgtaatag ttggcaaaag agaaagtatg ccaggagtcc
   107821 agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat catttgtatt
   107881 cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg atgatagagg
   107941 gtgaaaattg acccctctgt tcaaaaaaat tgaactattc gaggggccat caaacttact
   108001 tcgcgagagt cactgtggag ggagaggctt gctcgaatcc acgttcaagt atttccagat
   108061 tcgtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg gtttgataca
   108121 acgcatcata aacgcgcccg taatcgccca tctccggctt catctcttct ctgaccgtca
   108181 cgccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg aatcccggtt
   108241 cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgatacca tatttaataa
   108301 acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc agatggctgg
   108361 ttttgacgat ggcttttaga tcgccataaa acagttgcgc ttcgaaagta tcgtcaggat
   108421 tggctttatt gcgcaggctg cggatgtcat aagcgacgtg atccgggcga ccgaacagag
   108481 aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc tgcggcagcc
   108541 caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact tcgacaatct
   108601 ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa tcaaagcgac
   108661 gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac agctctttcg
   108721 cctgcgcaag tgtcggagtg aacggttttt cgaccagcac atttttcccg gcttccatcg
   108781 cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc agcttaacat
   108841 cgggatcgtt tagcacttcg ttgagatcgc tggtgaaatg gatatgagag taaatgggag
   108901 cttgttcttc cggctttgca tggcggcgaa aaatatgcgc gacatgccag ctatccttgc
   108961 ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca ataaaggcgc
   109021 agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag cgcaaagcgg
   109081 acgtggttcc tactggagtg cgcataacga acacaagcac ttcccgagga taaattgaga
   109141 acgaaagatc aaaaaaaaag ccagcacccg gctggctaag taatactgga agcaatgtga
   109201 gcaatgtcgt gctttcaggt tctccgcgag ggtcttcctg atcgcgagac aataataatc
   109261 attctcattc gcacttgtcc aacacttttt gcaaaaaaat gcatttgact cgcattcgaa
   109321 agtcaatgat gttgaaaggg acatttaccc caaagaggac aaaggaatga gtgagatagt
   109381 gatacgccat gctgaaacac gggattacga ggccatcagg cagattcacg cccagccgga
   109441 ggtgtattac aacacactac aggtgcctca tccttccgat catatgtggc aggagcgact
   109501 cgccgatcgt cccggcatca agcaactcgt cgcctgtatt gatggagacg tcgtgggcca
   109561 tctcaccatt gacgtgcaac agcgcccacg ccgcagtcat gttgccgatt ttggtatctg
   109621 tgtcgacgcc cgctggaaga atcgcggcgt cgccagcgcc ctgatgcgag agatgattga
   109681 aatgtgcgac aactggttgc gggtagatcg tattgagcta acggtgttcg tcgataacgc
   109741 gccagcaatt aaggtctata aaaaattcgg ctttgagatt gaagggactg gcaagaagta
   109801 cgcattacgt aatggtgaat atgttgatgc gtattttatg gcgcgggtga agtaaagaat
   109861 attctcaaat tgcctgttac caatgtgaat aattagtttt gttcatctaa tttgagtttt
   109921 t
//
LOCUS       DAZKIF010000002        93591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZKIF010000002 DAZKIF010000000
VERSION     DAZKIF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 93591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.002"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..760
                     /locus_tag="ACHOTW_000100"
     CDS             <1..760
                     /locus_tag="ACHOTW_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFP9842291.1"
                     /translation="SQRFGLRIAGVVSLLFIAAIALMVIPENGILRDPINHTVMPSPF
                     IKGIVPLIILFFFVVSLAYGIATRTIRRQADLPHLMIEPMKEMAGFIVMVFPLAQFVA
                     MFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSILAPIF
                     VPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGTYYSLV
                     LPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
     gene            771..1286
                     /gene="ogt"
                     /locus_tag="ACHOTW_000101"
     CDS             771..1286
                     /gene="ogt"
                     /locus_tag="ACHOTW_000101"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9842292.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            1481..2233
                     /gene="fnr"
                     /locus_tag="ACHOTW_000102"
     CDS             1481..2233
                     /gene="fnr"
                     /locus_tag="ACHOTW_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP9842293.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            2385..3335
                     /gene="uspE"
                     /locus_tag="ACHOTW_000103"
     CDS             2385..3335
                     /gene="uspE"
                     /locus_tag="ACHOTW_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP9842294.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(3385..3642)
                     /gene="ynaJ"
                     /locus_tag="ACHOTW_000104"
     CDS             complement(3385..3642)
                     /gene="ynaJ"
                     /locus_tag="ACHOTW_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP9842295.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            3886..4917
                     /gene="ynaI"
                     /locus_tag="ACHOTW_000105"
     CDS             3886..4917
                     /gene="ynaI"
                     /locus_tag="ACHOTW_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP9842296.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(4968..6581)
                     /gene="mppA"
                     /locus_tag="ACHOTW_000106"
     CDS             complement(4968..6581)
                     /gene="mppA"
                     /locus_tag="ACHOTW_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP9842297.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(6917..7816)
                     /gene="pgrR"
                     /locus_tag="ACHOTW_000107"
     CDS             complement(6917..7816)
                     /gene="pgrR"
                     /locus_tag="ACHOTW_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP9842298.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            7954..8874
                     /locus_tag="ACHOTW_000108"
     CDS             7954..8874
                     /locus_tag="ACHOTW_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9842299.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            8874..9804
                     /locus_tag="ACHOTW_000109"
                     /pseudo
     CDS             8874..9804
                     /locus_tag="ACHOTW_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            9825..10463
                     /locus_tag="ACHOTW_000110"
     CDS             9825..10463
                     /locus_tag="ACHOTW_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9842300.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            10754..11482
                     /gene="mpaA"
                     /locus_tag="ACHOTW_000111"
     CDS             10754..11482
                     /gene="mpaA"
                     /locus_tag="ACHOTW_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP9842301.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(11457..12422)
                     /gene="ycjG"
                     /locus_tag="ACHOTW_000112"
     CDS             complement(11457..12422)
                     /gene="ycjG"
                     /locus_tag="ACHOTW_000112"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP9842302.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            12541..13047
                     /gene="tpx"
                     /locus_tag="ACHOTW_000113"
     CDS             12541..13047
                     /gene="tpx"
                     /locus_tag="ACHOTW_000113"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP9842303.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(13091..14632)
                     /gene="tyrR"
                     /locus_tag="ACHOTW_000114"
     CDS             complement(13091..14632)
                     /gene="tyrR"
                     /locus_tag="ACHOTW_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP9842304.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(14780..15841)
                     /gene="ycjF"
                     /locus_tag="ACHOTW_000115"
     CDS             complement(14780..15841)
                     /gene="ycjF"
                     /locus_tag="ACHOTW_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP9842305.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(15838..17235)
                     /gene="ycjX"
                     /locus_tag="ACHOTW_000116"
     CDS             complement(15838..17235)
                     /gene="ycjX"
                     /locus_tag="ACHOTW_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP9842306.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            17390..18388
                     /gene="ycjW"
                     /locus_tag="ACHOTW_000117"
     CDS             17390..18388
                     /gene="ycjW"
                     /locus_tag="ACHOTW_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9842307.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            complement(18499..19404)
                     /gene="ompG"
                     /locus_tag="ACHOTW_000118"
     CDS             complement(18499..19404)
                     /gene="ompG"
                     /locus_tag="ACHOTW_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP9842308.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(19449..20531)
                     /locus_tag="ACHOTW_000119"
     CDS             complement(19449..20531)
                     /locus_tag="ACHOTW_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9842309.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(20545..21204)
                     /gene="pgmB"
                     /locus_tag="ACHOTW_000120"
     CDS             complement(20545..21204)
                     /gene="pgmB"
                     /locus_tag="ACHOTW_000120"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9842310.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(21201..23468)
                     /gene="ycjT"
                     /locus_tag="ACHOTW_000121"
     CDS             complement(21201..23468)
                     /gene="ycjT"
                     /locus_tag="ACHOTW_000121"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP9842311.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(23465..24520)
                     /gene="ycjS"
                     /locus_tag="ACHOTW_000122"
     CDS             complement(23465..24520)
                     /gene="ycjS"
                     /locus_tag="ACHOTW_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9842312.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(24530..25318)
                     /gene="ycjR"
                     /locus_tag="ACHOTW_000123"
     CDS             complement(24530..25318)
                     /gene="ycjR"
                     /locus_tag="ACHOTW_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9842313.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(25336..26388)
                     /gene="ycjQ"
                     /locus_tag="ACHOTW_000124"
     CDS             complement(25336..26388)
                     /gene="ycjQ"
                     /locus_tag="ACHOTW_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9842314.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(26419..27261)
                     /gene="ycjP"
                     /locus_tag="ACHOTW_000125"
     CDS             complement(26419..27261)
                     /gene="ycjP"
                     /locus_tag="ACHOTW_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9842315.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(27248..28129)
                     /gene="ycjO"
                     /locus_tag="ACHOTW_000126"
     CDS             complement(27248..28129)
                     /gene="ycjO"
                     /locus_tag="ACHOTW_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9842316.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(28150..29442)
                     /gene="ycjN"
                     /locus_tag="ACHOTW_000127"
                     /pseudo
     CDS             complement(28150..29442)
                     /gene="ycjN"
                     /locus_tag="ACHOTW_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(29456..31134)
                     /gene="ycjM"
                     /locus_tag="ACHOTW_000128"
                     /pseudo
     CDS             complement(29456..31134)
                     /gene="ycjM"
                     /locus_tag="ACHOTW_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(31344..31658)
                     /gene="pspE"
                     /locus_tag="ACHOTW_000129"
     CDS             complement(31344..31658)
                     /gene="pspE"
                     /locus_tag="ACHOTW_000129"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP9842317.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(31733..31954)
                     /gene="pspD"
                     /locus_tag="ACHOTW_000130"
     CDS             complement(31733..31954)
                     /gene="pspD"
                     /locus_tag="ACHOTW_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP9842318.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(31963..32322)
                     /gene="pspC"
                     /locus_tag="ACHOTW_000131"
     CDS             complement(31963..32322)
                     /gene="pspC"
                     /locus_tag="ACHOTW_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP9842319.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(32322..32546)
                     /gene="pspB"
                     /locus_tag="ACHOTW_000132"
     CDS             complement(32322..32546)
                     /gene="pspB"
                     /locus_tag="ACHOTW_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP9842320.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(32600..33268)
                     /gene="pspA"
                     /locus_tag="ACHOTW_000133"
     CDS             complement(32600..33268)
                     /gene="pspA"
                     /locus_tag="ACHOTW_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP9842321.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            33435..34412
                     /gene="pspF"
                     /locus_tag="ACHOTW_000134"
     CDS             33435..34412
                     /gene="pspF"
                     /locus_tag="ACHOTW_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP9842322.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(34543..35808)
                     /gene="puuE"
                     /locus_tag="ACHOTW_000135"
     CDS             complement(34543..35808)
                     /gene="puuE"
                     /locus_tag="ACHOTW_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP9842323.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(35846..37126)
                     /gene="puuB"
                     /locus_tag="ACHOTW_000136"
     CDS             complement(35846..37126)
                     /gene="puuB"
                     /locus_tag="ACHOTW_000136"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP9842324.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     GVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(37128..38615)
                     /gene="puuC"
                     /locus_tag="ACHOTW_000137"
     CDS             complement(37128..38615)
                     /gene="puuC"
                     /locus_tag="ACHOTW_000137"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP9842325.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(38765..39322)
                     /gene="puuR"
                     /locus_tag="ACHOTW_000138"
     CDS             complement(38765..39322)
                     /gene="puuR"
                     /locus_tag="ACHOTW_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP9842326.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(39349..40113)
                     /gene="puuD"
                     /locus_tag="ACHOTW_000139"
     CDS             complement(39349..40113)
                     /gene="puuD"
                     /locus_tag="ACHOTW_000139"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP9842327.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            40325..41743
                     /gene="puuA"
                     /locus_tag="ACHOTW_000140"
     CDS             40325..41743
                     /gene="puuA"
                     /locus_tag="ACHOTW_000140"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFP9842328.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            41892..42056
                     /locus_tag="ACHOTW_000141"
     CDS             41892..42056
                     /locus_tag="ACHOTW_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9842329.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            42046..43431
                     /gene="puuP"
                     /locus_tag="ACHOTW_000142"
     CDS             42046..43431
                     /gene="puuP"
                     /locus_tag="ACHOTW_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP9842330.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            43565..43810
                     /gene="ymjA"
                     /locus_tag="ACHOTW_000143"
     CDS             43565..43810
                     /gene="ymjA"
                     /locus_tag="ACHOTW_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFP9842331.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            44123..45766
                     /gene="sapA"
                     /locus_tag="ACHOTW_000144"
     CDS             44123..45766
                     /gene="sapA"
                     /locus_tag="ACHOTW_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP9842332.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLTHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            45763..46728
                     /gene="sapB"
                     /locus_tag="ACHOTW_000145"
     CDS             45763..46728
                     /gene="sapB"
                     /locus_tag="ACHOTW_000145"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP9842333.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            46715..47605
                     /gene="sapC"
                     /locus_tag="ACHOTW_000146"
     CDS             46715..47605
                     /gene="sapC"
                     /locus_tag="ACHOTW_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP9842334.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            47605..48597
                     /gene="sapD"
                     /locus_tag="ACHOTW_000147"
     CDS             47605..48597
                     /gene="sapD"
                     /locus_tag="ACHOTW_000147"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP9842335.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            48599..49405
                     /gene="sapF"
                     /locus_tag="ACHOTW_000148"
     CDS             48599..49405
                     /gene="sapF"
                     /locus_tag="ACHOTW_000148"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP9842336.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(49457..50620)
                     /locus_tag="ACHOTW_000149"
     CDS             complement(49457..50620)
                     /locus_tag="ACHOTW_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFP9842337.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(50629..52002)
                     /locus_tag="ACHOTW_000150"
     CDS             complement(50629..52002)
                     /locus_tag="ACHOTW_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFP9842338.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(52006..55108)
                     /locus_tag="ACHOTW_000151"
                     /pseudo
     CDS             complement(52006..55108)
                     /locus_tag="ACHOTW_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(55108..56229)
                     /locus_tag="ACHOTW_000152"
     CDS             complement(55108..56229)
                     /locus_tag="ACHOTW_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9842339.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(56378..56944)
                     /locus_tag="ACHOTW_000153"
     CDS             complement(56378..56944)
                     /locus_tag="ACHOTW_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9842340.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            57422..58210
                     /gene="fabI"
                     /locus_tag="ACHOTW_000154"
     CDS             57422..58210
                     /gene="fabI"
                     /locus_tag="ACHOTW_000154"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9842341.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            58354..59481
                     /gene="yciW"
                     /locus_tag="ACHOTW_000155"
     CDS             58354..59481
                     /gene="yciW"
                     /locus_tag="ACHOTW_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFP9842342.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            59549..61483
                     /gene="rnb"
                     /locus_tag="ACHOTW_000156"
     CDS             59549..61483
                     /gene="rnb"
                     /locus_tag="ACHOTW_000156"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP9842343.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(61602..61664)
                     /locus_tag="ACHOTW_000157"
                     /pseudo
     CDS             complement(61602..61664)
                     /locus_tag="ACHOTW_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            61718..63703
                     /gene="pdeR"
                     /locus_tag="ACHOTW_000158"
     CDS             61718..63703
                     /gene="pdeR"
                     /locus_tag="ACHOTW_000158"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9842344.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTAY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(63685..63813)
                     /gene="ymiD"
                     /locus_tag="ACHOTW_000159"
     CDS             complement(63685..63813)
                     /gene="ymiD"
                     /locus_tag="ACHOTW_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP9842345.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            63851..64024
                     /gene="yciZ"
                     /locus_tag="ACHOTW_000160"
     CDS             63851..64024
                     /gene="yciZ"
                     /locus_tag="ACHOTW_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP9842346.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            64114..64863
                     /gene="yciT"
                     /locus_tag="ACHOTW_000161"
     CDS             64114..64863
                     /gene="yciT"
                     /locus_tag="ACHOTW_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP9842347.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            65132..65350
                     /gene="osmB"
                     /locus_tag="ACHOTW_000162"
     CDS             65132..65350
                     /gene="osmB"
                     /locus_tag="ACHOTW_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP9842348.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(65476..65802)
                     /gene="yciH"
                     /locus_tag="ACHOTW_000163"
     CDS             complement(65476..65802)
                     /gene="yciH"
                     /locus_tag="ACHOTW_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP9842349.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(65802..66539)
                     /gene="pyrF"
                     /locus_tag="ACHOTW_000164"
     CDS             complement(65802..66539)
                     /gene="pyrF"
                     /locus_tag="ACHOTW_000164"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9842350.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(66732..67901)
                     /gene="lapB"
                     /locus_tag="ACHOTW_000165"
     CDS             complement(66732..67901)
                     /gene="lapB"
                     /locus_tag="ACHOTW_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP9842351.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(67908..68216)
                     /gene="lapA"
                     /locus_tag="ACHOTW_000166"
     CDS             complement(67908..68216)
                     /gene="lapA"
                     /locus_tag="ACHOTW_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP9842352.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            complement(68365..69129)
                     /gene="pgpB"
                     /locus_tag="ACHOTW_000167"
     CDS             complement(68365..69129)
                     /gene="pgpB"
                     /locus_tag="ACHOTW_000167"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP9842353.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            69299..69889
                     /gene="ribA"
                     /locus_tag="ACHOTW_000168"
     CDS             69299..69889
                     /gene="ribA"
                     /locus_tag="ACHOTW_000168"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP9842354.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(69953..72628)
                     /gene="acnA"
                     /locus_tag="ACHOTW_000169"
     CDS             complement(69953..72628)
                     /gene="acnA"
                     /locus_tag="ACHOTW_000169"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP9842355.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(72792..72887)
                     /gene="ymiC"
                     /locus_tag="ACHOTW_000170"
     CDS             complement(72792..72887)
                     /gene="ymiC"
                     /locus_tag="ACHOTW_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP9842356.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(73001..73168)
                     /gene="yciX"
                     /locus_tag="ACHOTW_000171"
     CDS             complement(73001..73168)
                     /gene="yciX"
                     /locus_tag="ACHOTW_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP9842357.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(73171..73311)
                     /gene="ymiA"
                     /locus_tag="ACHOTW_000172"
     CDS             complement(73171..73311)
                     /gene="ymiA"
                     /locus_tag="ACHOTW_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP9842358.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(73630..74604)
                     /gene="cysB"
                     /locus_tag="ACHOTW_000173"
     CDS             complement(73630..74604)
                     /gene="cysB"
                     /locus_tag="ACHOTW_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP9842359.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(74814..77411)
                     /gene="topA"
                     /locus_tag="ACHOTW_000174"
     CDS             complement(74814..77411)
                     /gene="topA"
                     /locus_tag="ACHOTW_000174"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9842360.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            77791..78042
                     /gene="yciN"
                     /locus_tag="ACHOTW_000175"
     CDS             77791..78042
                     /gene="yciN"
                     /locus_tag="ACHOTW_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP9842361.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(78078..79127)
                     /gene="sohB"
                     /locus_tag="ACHOTW_000176"
     CDS             complement(78078..79127)
                     /gene="sohB"
                     /locus_tag="ACHOTW_000176"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP9842362.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            79347..80105
                     /gene="yciK"
                     /locus_tag="ACHOTW_000177"
     CDS             79347..80105
                     /gene="yciK"
                     /locus_tag="ACHOTW_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP9842363.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            80102..80692
                     /gene="cobO"
                     /locus_tag="ACHOTW_000178"
     CDS             80102..80692
                     /gene="cobO"
                     /locus_tag="ACHOTW_000178"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP9842364.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(80732..81604)
                     /gene="rluB"
                     /locus_tag="ACHOTW_000179"
     CDS             complement(80732..81604)
                     /gene="rluB"
                     /locus_tag="ACHOTW_000179"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP9842365.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(81817..83400)
                     /locus_tag="ACHOTW_000180"
     CDS             complement(81817..83400)
                     /locus_tag="ACHOTW_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFP9842366.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            complement(83428..84048)
                     /gene="yciO"
                     /locus_tag="ACHOTW_000181"
     CDS             complement(83428..84048)
                     /gene="yciO"
                     /locus_tag="ACHOTW_000181"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9842367.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(84045..84926)
                     /gene="rnm"
                     /locus_tag="ACHOTW_000182"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(84045..84926)
                     /gene="rnm"
                     /locus_tag="ACHOTW_000182"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP9842368.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            85064..85108
                     /gene="trpL"
                     /locus_tag="ACHOTW_000183"
     CDS             85064..85108
                     /gene="trpL"
                     /locus_tag="ACHOTW_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP9842369.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    85085..85178
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            85200..86762
                     /gene="trpE"
                     /locus_tag="ACHOTW_000184"
     CDS             85200..86762
                     /gene="trpE"
                     /locus_tag="ACHOTW_000184"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP9842370.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVGPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            86762..88357
                     /gene="trpD"
                     /locus_tag="ACHOTW_000185"
     CDS             86762..88357
                     /gene="trpD"
                     /locus_tag="ACHOTW_000185"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP9842371.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            88358..89719
                     /gene="trpCF"
                     /locus_tag="ACHOTW_000186"
     CDS             88358..89719
                     /gene="trpCF"
                     /locus_tag="ACHOTW_000186"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP9842372.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            89731..90924
                     /gene="trpB"
                     /locus_tag="ACHOTW_000187"
     CDS             89731..90924
                     /gene="trpB"
                     /locus_tag="ACHOTW_000187"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP9842373.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            90924..91730
                     /gene="trpA"
                     /locus_tag="ACHOTW_000188"
     CDS             90924..91730
                     /gene="trpA"
                     /locus_tag="ACHOTW_000188"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP9842374.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            92111..92290
                     /gene="yciG"
                     /locus_tag="ACHOTW_000189"
     CDS             92111..92290
                     /gene="yciG"
                     /locus_tag="ACHOTW_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9842375.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            92376..92876
                     /gene="yciF"
                     /locus_tag="ACHOTW_000190"
     CDS             92376..92876
                     /gene="yciF"
                     /locus_tag="ACHOTW_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9842376.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            92922..93428
                     /gene="yciE"
                     /locus_tag="ACHOTW_000191"
     CDS             92922..93428
                     /gene="yciE"
                     /locus_tag="ACHOTW_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9842377.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(93488..>93591)
                     /locus_tag="ACHOTW_000192"
     CDS             complement(93488..>93591)
                     /locus_tag="ACHOTW_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFP9842378.1"
                     /translation="IDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT        22992 a        24509 c        22890 g        23200 t
ORIGIN      
        1 aagtcagcgt tttggtttac gcatagcagg tgtcgtatcg ctacttttta ttgctgcgat
       61 tgcgctgatg gtgatcccgg aaaacgggat attgcgcgat ccgattaatc acaccgtgat
      121 gccatcaccc tttattaaag gtatcgtgcc actgatcatt ctttttttct ttgttgtctc
      181 gctggcttat ggcatcgcta cccgcacaat tcgacgtcag gcggatttac cgcatttaat
      241 gattgaaccg atgaaagaga tggcgggatt tatcgtgatg gtttttcccc tcgcccagtt
      301 tgtcgccatg tttaactgga gcaacatggg gaaattcatc gccgtggggc tgaccgatat
      361 cctggaaagt tcagggctta gcggcatccc ggcgtttgtc ggtctggcgt tgctttcctc
      421 tttcttatgc atgtttatcg ccagcggttc cgcaatctgg tcgattctgg cccccatttt
      481 cgtaccaatg tttatgctac ttggctttca cccggcattt gcgcaaatcc tctttcgtat
      541 tgccgactca tccgtattgc ctttagcgcc agtatctcct tttgttccac tgtttcttgg
      601 attcctgcaa cgctacaaac cagacgcgaa actgggtact tactattcgt tagtcttgcc
      661 ctatccgctt atctttttgg tggtatggct gctgatgttg ctggcgtggt atcttgtggg
      721 cctgccgata ggtccgggta tttacccacg tttgtcttaa gagagaacgg atgctgagat
      781 tacttgaaga aaaaattgcc acaccactgg gtccactgtg ggtgatttgc gatgagcaat
      841 ttcgcctgcg ggcggttgaa tgggaagagt acagcgaacg catggtgcag ctgctggaca
      901 tccattatcg caaagaaggc tatgagcgca tttctgccac caatccaggc ggtttaagcg
      961 acaagcttcg tgaatatttt gccggtaatc ttagcattat tgatacgctt cccactgcta
     1021 cgggggggac gccatttcag cgcgaagtct ggaaaacact acgcactatc ccctgcgggc
     1081 aggtaatgca ttacggcgaa ctggctgagc aattgggccg tcctggcgcg gcgcgtgccg
     1141 ttggtgcggc aaacggatcg aatcccatca gcatcgtcgt accttgccat cgggttattg
     1201 gccgaaacgg caccatgacc ggatatgcag gcggagttca gcgaaaagag tggttattgc
     1261 gccacgaagg ttatcttttg ctgtaaacat taaacaattt gtgccagctt gttcacactt
     1321 ttatgtaaag ttacccttaa caacttaagg gttttcaaat agatagacat atatttacat
     1381 ctaatatcgg aattctctgc tgttaaggtt tgcttagact tacttgctcc ctaaaaagat
     1441 gttaaaattg acaaatatca attacggctt gagcagacct atgatcccgg aaaagcgaat
     1501 tatacggcgc attcagtctg gcggttgtgc tatccattgc caggattgca gcatcagcca
     1561 gctttgcatc ccgttcacac tcaacgaaca tgagcttgat cagcttgata atatcattga
     1621 gcggaagaag cctattcaga aaggccagac gctgtttaag gctggtgatg aacttaaatc
     1681 gctttatgcc atccgctccg gtacgattaa aagttatacc atcactgagc aaggcgacga
     1741 gcaaatcact ggtttccatt tagcaggcga cctggtggga tttgacgcca tcggcagcgg
     1801 ccatcacccg agcttcgcgc aggcgctgga aacctcgatg gtatgtgaaa tcccgttcga
     1861 aacgctggac gatttgtccg gtaaaatgcc gaatctgcgt cagcagatga tgcgtctgat
     1921 gagcggtgaa atcaaaggcg atcaggacat gatcctgctg ttgtcaaaga aaaatgccga
     1981 ggaacgtctg gctgcattca tctacaacct gtcccgtcgt tttgcccaac gcggcttctc
     2041 ccctcgtgaa ttccgcctga cgatgactcg tggcgatatc ggtaactatc tgggcctgac
     2101 ggtagaaacc atcagccgtc tgctgggtcg cttccagaaa agcggtatgc tggcagtcaa
     2161 aggtaaatac atcaccatcg aaaataacga tgcgctggcc cagcttgctg gtcatacgcg
     2221 taacgttgcc tgatttttcc gcataactca ctatccttct gtcatatcat taaatttttc
     2281 tgatttattg atctggcaga aggttcatca ctgtttcatt caccagatat gggttaatct
     2341 tttaattaca aactgcgttg acagttgttg taaggagacc ctgtatggct atgtatcaga
     2401 acatgctcgt tgttatcgat cctaaccagg acgaccaacc agcattgcgg cgagctgttt
     2461 atttacatca acggattggt ggcaaaatta aagccttttt gccgatctat gacttctcat
     2521 acgaaatgac caccctgctc tccccggacg aacgtaccgc tatgcgtcag ggcgtcatca
     2581 gccagcgtac agcctggatc cacgagcagg caaaatatta tctcaatgct ggcgttccca
     2641 ttgaaattaa agtggtctgg cataaccgtc ctttcgaagc catcattcag gaagtgatca
     2701 gcggcgggca cgatttggtg ctaaaaatgg cgcaccaaca tgaccgtctg gaagcggtga
     2761 tttttacgcc aacggactgg cacctgttac gcaaatgccc aagcccggtg tggatggtga
     2821 aagaccagcc gtggccggaa ggaggtaagg cgctggtggc ggtgaatctc gccagtgaag
     2881 agccgtatca taatgcgctc aatgaaaaac tggtcaaaga gacgatcgaa ctggcagaac
     2941 aagtcaacca taccgaagtt catctggttg gcgcttatcc ggtaacgcca attaatatcg
     3001 cgattgaact gccggaattt gatccgagcg tttataacga tgccattcgt gggcaacatt
     3061 tgctggcaat gaaagccctg cggcagaaat tcggcattaa tgaaaacatg acgcacgtag
     3121 aaaaaggtct gccagaagag gtgattcctg atttggcgga gcacttacag gcggggattg
     3181 tggttctggg cacggtagga cgcaccggta tttcagcagc attcctcggc aacacggcgg
     3241 aacaggtgat tgatcatctt cgctgcgatc tgttggtcat taaacctgac cagtatcaga
     3301 cacccgttga actggatgac gaagaagacg attaacgact cctcaataac tgacaaggcc
     3361 cgagttatcg ggcctatttt ttacttactg ctcttcgccg ccaaggaaat aaattcccaa
     3421 cgggattgcc agcaacacag taaagaccag gctataaaca aaaatcatcg atgactgaat
     3481 cacatacatt gatgtcgtcc actccgacag cggaatattg tactgttcta taacgccgcc
     3541 gatagtcgcg cgagtcacaa ccgacgccgc aataatgaaa accgccaaca aaccaaagag
     3601 aaatttcttc cctttcgctg acttcagttt cgccataatc atgatgtaat ccttgtgcag
     3661 acaaatttct tgttacctaa acgtaacaat accatgacat ttcaccattg tctgctctga
     3721 aaacctcacc tcttagctgg atttacaatc aacgcattga ttagttagct aatgttgcta
     3781 cttccttccc tgactattga tgatctgaat gaattgtcgc ggtaaaatag ccccgccagg
     3841 ggtttccggc atgacgacaa ttatctggag gagcctgata aaacaatgat cgctgaactg
     3901 tttacaaata atgcgcttaa tctggtcatt attttcggta gctgcgcagc attgattctg
     3961 atgagctttt ggtttcgccg cggaaatcgt aaaagaaaag gctttttatt ccatgcggtg
     4021 caatttttaa tctacaccat aattatcagt gctgttggta gcattattaa ttatgttata
     4081 gaaaactata aactcaaatt tatcactcca ggcgttatcg attttatctg tacgtccctg
     4141 attgcggtta ttctgacgat taagctattt ttgctgatta atcagtttga aaaacagcag
     4201 attaaaaagg gtcgcgatat caccagtgcg cggattatgt cgcgcattat caaaatcacc
     4261 attattgtgg tgcttgttct gctttatggc gaacatttcg gcatgagcct ttctggcttg
     4321 ctgacctttg gtggtattgg tggtctggct gtcggtatgg ctggcaaaga tattctgagt
     4381 aatttctttt ccgggatcat gctctatttc gaccgtcctt tcagtattgg cgactggatc
     4441 cgttcaccgg acagaaatat cgaaggtaca gtagcggaaa ttggctggcg aattaccaaa
     4501 attacgacct ttgataatcg tccattgtac gtaccgaact cgctgttttc atcgatcagc
     4561 gtagaaaacc caggacgaat gaccaaccgc cgcattacca cgaccattgg tttacgttat
     4621 gaggatgcgg caaaagtggg cgttatcgtc gaagccgtac gtgagatgct gaaaaatcac
     4681 ccggccatcg accagcgaca aaccttactg gtttatttca accagtttgc tgactcgtca
     4741 ttgaatatta tggtttattg ctttaccaaa accacggtat gggctgagtg gcttgctgca
     4801 cagcaagacg tttatttgaa gattatcgat attgtacagt cacacggcgc ggactttgcc
     4861 ttccccagcc agacgctgta tatggataac attacaccgc cggaacaggg tcgctaaatc
     4921 agacatcact ggcgttatcc acaagggtaa cgccagttcc catcacatca atgcttcaca
     4981 atatacatag tccgactgta cgccacatcc tcagggttat taatgggata acctttcagc
     5041 cacggtttga ttaatcgtcc attggtatat tgataaattg gtgcaatcgg tgcttgctcc
     5101 atgaggattt tttctgccgc gttgtaatcg gcattacgcg ctttaacggt attttccgtc
     5161 gatgcctggg ccagaacttt gtcgtatgcc ggattgttaa agcgtgaaat atttcctgaa
     5221 tgcgttgacg ttaataatgt caggaaagtg gagggttcat tataatcccc cacccatgag
     5281 gcgcggataa catcaaaatt gccggtgtta cggctatcga tataggtttt ccattcctga
     5341 ttttgcaatt taacatctac gccaaggttc tttttccaca tcgatgctac agcaatcgca
     5401 attttttgat ggttttctga agtgttatac aaaagcgtca gcttcagcgg tttttgcgga
     5461 ccataaccag ctgcgctcaa caaagttttt gcctgcgcat tcagttcttc ctgactcatt
     5521 tgttcaaacg gcgaaggttc cggcgtaaat ccggcggtaa catctggtgt aaaatgccac
     5581 gctggctttt cgcccgtccc taatactttt tcggtcatca ggcggcgatc tatcgtcata
     5641 cttaatgcca gacgaacgcg ctgatctgcc gtcggccctt tttgcgtgtt aaacgcataa
     5701 taataggtcc cgagctgcgg cggcgtataa acctgccccg gaatatcctt caacagcttc
     5761 tgatacatat ttttcgggaa ggattcggtg atatcaatat cccccgcgag gtaacgctta
     5821 gtggctgcgg attcctgatt aattggcagg aaggtcactt tttgcagtac cgttttggcg
     5881 ttatcccaat aatgggtatt cggtaccacg accagttttt cattgactac gcgctcttta
     5941 agaacataag cgccattgcc gatcagattt ccgggtttcg tccactcttt accgctttct
     6001 acgttggctt tttgcaccgg gaagaaggca aagttagcgg ttaaattcac aaaccacggc
     6061 aacggtttat caagctgaat tttcaaagta tggacatcaa ctgcggtgac gccaagctgg
     6121 tcaggtgtag ctttaccatc aataatcgcc tgtgcgttgt tgattcccgc cagcgcggca
     6181 aaccatgcaa atggcgatag cgttttcggg tccaccagac gctgccagct gtagacaaaa
     6241 tcttgcgccg ttaccggtgt gccatccgcc cattttgcgt tatcgcgcag ggtaaaagtc
     6301 cagatacggt tgtcattact tttccactga gtcgcaacgc cggggacaat ctcccctttc
     6361 tcgttctgat tcaccagtcc ttcaaacaga tcgcgaatga cctgaatctc tggcaggccc
     6421 acggctttag cgggatccag cgacgcaggc tcatctttaa tgtggcgcac cagctcctgc
     6481 ttctctgcca gtactgtgcc gctcggaact tctgcagcat acgaaagaga aatgctgctg
     6541 accaacagcg cacaacacgt gactgaaaca gagtgcttca taagtttccc tctaaccgac
     6601 attaaaattg atgcgtaatt atttgtttcg ccttaaagaa atgcaaacaa cttccatcag
     6661 aaagtgacac aggaagcata aaatgtttta tgtatcaata tattaaatca gatttagtgt
     6721 aaataataga cgccaaaatg cggcaaagca catagaacac cgaacactac gttcgggctt
     6781 cacacgttca aaccagtttt cggtatcata atcagcatct gtcgccattg tacgaagaat
     6841 aaaaaataac caaaacgttg aaaggaactg agaatacgtt aagagaagaa ttaaattaat
     6901 gtatcagcaa gtagtgctaa acagcacctt tatacttcag cctatctata aataatgaga
     6961 atgccgagcc agcatgtcga cggtgtggat agtacaggtg ataaccgggt aaatctggtg
     7021 tgaatttatc caaaacacgt atcagttttt tttctttaat cgcccgctca acctgatcat
     7081 aaggtagata cgccaatcca tgcccatcaa ttgcagcatc aatgatcagg tctatcgtat
     7141 tcagtaaaag ctgaccttcc atgcgaacac gaacttcacg tccaccgcgt attaatctcc
     7201 agcgatttgc tgtacccgat gtgggaagat acaaattaat tgcctgatga tctattaatt
     7261 gtgacactga cgttggaaca cttcggcgag aaaaataatc cggtgagcca acaatagcca
     7321 ttggtatatc tggcccgatt cgaatggcga tcatatcttt atccatctcc ccacccagac
     7381 ggacgcctgc atcaaaacgt tcagaaacga catcagtcaa accataatca atgataagct
     7441 gaatatcaat ttcagggtgt gatttcagga atgtgcgcat ggctggtaac aatatcgttt
     7501 ttgctgcatg ttctacagta gtaatacgta ttgtcccgga tgggcggttc tgcagatcgc
     7561 tcagggatgc cagggcagaa tctatgtcat gcagcatagg gccaagaaca gacaaaagat
     7621 gctcgcccgc ttcagtgggt acaacactgc gcgtggttcg cgtcagaagc cgcaatccca
     7681 atcgttcttc tatacggcgc actatctggc ttaaagctga ctgcgccatg ctcaggcggg
     7741 ctgctgcgcg agtgaagcta cgctcctctg caacgacgac aaacgccatc agatcagcga
     7801 tctcttcacg cttcattcag tatctccata caatctatta atcatttaaa tgataggtct
     7861 aatcatgatt tagggtctaa tcaacttaat gatcaatatc tatagtgata tcaccaaccg
     7921 ttaaaacgaa ccaacaactc catgaggaac ggaatgatga ataataaagt cagcttcact
     7981 aacagcaata atccaaccat ctctttgtct gcagtgatct attttcctcc aaaatttgat
     8041 gaaacccgtc aatatccggc aattgtggta tctcacccag gtgggggcgt taaagaacaa
     8101 acggccggga cctatgccga aaaactggcg gaaaagggat ttgtcactat tgcttatgac
     8161 gcatcttatc agggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt
     8221 actgaagacg tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc
     8281 cgaattggtg cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag
     8341 gatcgccgca tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgt
     8401 aatggttggg aaaacaatgt gaaatctatt gatgccctgc catacgttga agcgggttca
     8461 aacgccagaa ccagcgatat tagcagcgga gaatacgcca ttatgccact ggcaccaatg
     8521 aaggagtccg atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacccct
     8581 cgcgcgcagt atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt
     8641 acctatgatg cttaccatat ggcggaagtg tacctgactc agccgacgca gattgtggcg
     8701 ggcagccagg cagggagcaa atggatgagt gacgacctgt acgatcgggc atcaagccag
     8761 gataaacgct atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc
     8821 tatgtggctg aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac
     8881 atattcaaat ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac
     8941 caaattttga tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt
     9001 gtaaggagca gacgtctggc aatatctatg gcaaggcatt ggctgaaaaa ggctatgtcg
     9061 ttctcgctta tgacgccagt ttccagggag aatctggtgg ggagcctcgt tggatagaag
     9121 atccgacaca gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat
     9181 atgttgatgc tgaacgcatt ggcgtgctgg gtgtgtgtgg gggggggtgg ctatgccatt
     9241 aatgccgcgc tgacagaaaa acgaattaaa gctgtagtga gtattacggg tgttaatatc
     9301 gggcgtcttt ttcgggaagg tttcagcaat tacgatccga ttggcgcatt gaatgcaatg
     9361 gcgtcgcaac gaaccaaaga ggctcgtggt ggagaattac agatcaacga attacttccc
     9421 gccagcctgg atgcagcaaa agcccatgga ctgaccgaac gcgatgttta tgaagcaact
     9481 gactactata aaacaccacg cgggcaacaa ccgggaggtg cgacaaaaat gttgttctca
     9541 catgcacaga aaacgctggc ctgggatgcc ttcgcattta ctgaagtgtt actgactcag
     9601 ccggtgatgg tagtagttgg tgaaaaagtc ggtgccttcg gtgcttatcg tgatggactg
     9661 gaagtctatg gccgggcgat ggtttcgcag gatcgtcagt tagtttcatt acccgatttc
     9721 tcacattatg aactgtatga caaaccggaa gcagttcagg aggcgctgga aaaagttatt
     9781 ccttttttca acacccatct gtgataagtt tcatgagaaa tattatgaaa aatgtactca
     9841 ttcttggtgc tggcggtcag atagcccgcc atgtgattaa tcaactggca gataaacaaa
     9901 ccattaaaca aactttattt gctcgtcagc cagcaaaaat acacaaaccc tatccaacta
     9961 atagccagat tattatgggg gatgtactga atcatgcagc actgaagcag gccatgcagg
    10021 gacaggatat tgtgtatgcc aaccttacgg gggaagattt agatattcag gcaaattcag
    10081 tgatcgctgc gatgaaagcc tgtgatgtta aacgtctgat ctttgttctt tccctcggca
    10141 tttatgatga agttccagga aaatttggcg aatggaataa tgcagttatt ggtgagcccc
    10201 taaaaccatt ccgcagggct gcggatgcaa ttgaggcttc tggactggaa tatacaattc
    10261 ttcgccctgc ctggctgacg gatgaagata ttattgatta tgagcttacc tcacgcaatg
    10321 aaccattcaa aggtacgatc gtatcaccta agagtgtcgc agcattaatt acagatatca
    10381 tcgataaacc tgagaaacac attggtgaga atataggcat caatcaaccc gggactgatg
    10441 gcgacaagcc attcttcatg taaatcaatt atataaccac catcgtactg tactatgtgc
    10501 agtacgattt attaaactta ttaaggtgaa ctgatcaatc agatgcgatt aacatgtgta
    10561 gaaccagtaa gaataaaata cgccatgtat ataacattac gacaattcta atatctgcac
    10621 atcaatgaaa atacaaacaa cttccggcaa aaaagtgata caagatcgat gacatagcgt
    10681 tgaagaaaag gatttgataa tccgcaaaat ttgcacaaca ctgcctgtaa tacttcctga
    10741 accagagacg atcatgaccg taacccgccc acgcgccgaa cgcggcgcat ttccgcccgg
    10801 aacagaacat tacggacgtt cattattggg cgcgccgtta atctggtttc cggcccctgc
    10861 agccagtcgc gaaagtggtt tgattctggc tggcactcac ggtgatgaaa actcttcggt
    10921 cgtcacgctc tcctgtgctc tgcggacttt gacaccttct ttgcgccgcc atcatgtggt
    10981 gctgtgtgtg aatcctgacg gctgccagct ggggttacgg gccaatgcta atggtgtgga
    11041 tttaaacaga aactttccgg cggcgaactg gaaggaaggt gaaacggttt atcgctggaa
    11101 cagcgccgct gaagaacgtg atgtcgtatt actgacaggt gacaaacctg gctccgaacc
    11161 tgaaacccag gcgctgtgcc agcttataca ccgcattcaa cctgcctggg tggtctcatt
    11221 ccacgatcca ctggcctgta ttgaagatcc cagacacagc gaattaggcg aatggctggc
    11281 tcaggcgttt gaattgcctc tggtaaccag cgtcggttat gaaacgcctg gctctttcgg
    11341 cagttggtgt gcagatctga acttgcattg catcaccgca gagtttccgc ccatctcctc
    11401 cgacgaagcc agcgaaaaat atctctttgc gatggcaaac ttgctgcgct ggcatcctaa
    11461 agatgcaatt cgcccgtcgt gaactgaagc gccggttcca catctaccgc cagccaggtc
    11521 ggtccgtcaa gatcggcgaa actgacctgc ggcaccagcg gtaaagcggc gctaatggcc
    11581 cgagaggtac acaacatgca gcccagcatc agactgaaac cttgtgcacg cgcttcagtc
    11641 gccagcgcca gcgcttccgt cagacccccg gttttatcga gcttaatatt aaccatctca
    11701 tagcgccctt tcagcgcctt caaattgcta cgagtatgac aactttcatc agcacaaatc
    11761 ggcaacggat gaataaaatt ctccagcgcc gcatcgtcct gcgcaggaag cggttgttca
    11821 agcatcgcaa cgcctaaatc cgccaatagc tggcaacgtg ccgccaaccc ttctgcatgc
    11881 caggattcat ttgcatcaac gatcagcgtg gtatcgggca cagctgtgcg aattgccacc
    11941 atccgctcac tgataagatg gttatccagc ttcactttca gtaatttcgc gccggcctgc
    12001 cagagtgttg atgcactatt ggccatctga tcaggcgtac cgatgactac cgtctgtgca
    12061 gtgataactg tctcgggaag cgttatgccg atcaaatcag ccagcgattg ctgctgtttt
    12121 cgcgcggcca gatcccacaa tgcacaatcc agcgcattac gtgctgcgcc ggcagggaga
    12181 attttttgca actcctcccg tgtcagccct ttctctaatt gcggcacgac gctcataatt
    12241 tgcgccatta ccgaggcatc actttctcca taacgcggat acggcgtgca ttcgccggtg
    12301 cctttaatac cctcttcttc cagttcaacc accaccacgc gcgcttcact gcgacttccc
    12361 cgggcaatca caaacggggt atgtaagggc caggcttcct caaatacctt aacggttctc
    12421 atctttactc cttggtgtcg cggtggtctg cggattaagc gcaaatagcg tttgctgtgt
    12481 tattgacagt tagcataaac taggtgtgac gttaactata tgtaaacagg aaagataatc
    12541 atgtcacaaa ccgttcattt ccagggcaac ccggttacag tcgccaattc catcccgcag
    12601 gcgggtagca aagcgcagac ttttactctc gtggcaaaag atctgtctga cgtcaccctc
    12661 ggtcagtttg cgggtaaacg caaagtgctg aacattttcc cgagtattga taccggtgtt
    12721 tgcgccgcat cagtacgtaa gtttaaccaa ctggcaaccg agatcgacaa caccgttgtg
    12781 ctgtgtatct ctgccgatct gccgttcgcc cagtctcgtt tctgcggcgc agaaggtctg
    12841 aacaacgtta tcaccctctc cactttccgt aacgctgaat ttctgcaagc ctacggtgtg
    12901 gcaattgctg atggcccact gaaaggtctg gcagcgcgtg ccgttgtggt tattgatgaa
    12961 aatgacaatg tgattttcag ccagctggtg gatgaaatca ccaccgagcc ggattacgaa
    13021 gcagctctgg ctgtactgaa agcataagtt aatatgcctg atggtgttgc accatcaggc
    13081 atattcgcgc ttactcttcg ttcttcttct ggcttaagcc atattcccgc aacttattgg
    13141 caatcgcggt atgtgaaacg ccgagacgtt ttgccagttt gcgcgtgctg ggataattgc
    13201 gataaagctg ggttaatacc gagcgttcaa aacggctggt gatttcgtcc agcgaacctt
    13261 ccatcgcatc ttcgcccacg gctaccgttg cggcgtcata atccggcaac aaaatatcct
    13321 gtggacgcag ctcataaccg tccagttgtg tcagtgcgcg atagatagcg ttctttaact
    13381 gccgcacatt tcccggccac gcataacgcg taagtacagt attcaggtca gcggccagtt
    13441 tcggacgcgg cacgccctgc tcgtcggcaa agcgggcgac gaacagctca gttaacggca
    13501 tgatgtcctg cggacagtca cgtagcggcg gcaggttgag cgtcaacacg ttcagacgat
    13561 aatagagatc ttcacggaac atgccttttt gcaccagttc gaccagattc ttctgcgtag
    13621 cgcaaatcac ccgcacatcg acatgcacct catggtcttc gccaacccga cggaaagtgc
    13681 catcattaag gaaacgcagt aatttcgcct gcatccgtgg tgacatctca cctatttcat
    13741 ccaacagcac cgaaccaccg ttcgcctgct caaagaatcc tttcttccct tccggagcat
    13801 gaccaaacag ctcactctcg accgcatctt ccggtataga cgcacagttc agcgccaggt
    13861 aaggtttgct cgctcttggg cttgcctgat ggcaggcgta ggcaaaaaga tctttacctg
    13921 tacctgtgtc acccgtaatc agcagcggcg cgcttagcat cgccagtttc tgcgcctgtt
    13981 cgacaacatg cttcattttc gggctgacag cgacaatttg actgaaggcg ctgacgtcct
    14041 gggcggcgac attttgcaac tgacggccca tacgaatcgt tgatcgcaac atcaccaccg
    14101 cacctgtcag gacgtgttga tcattttcat cctgaagata aacaggcgta atctccatca
    14161 ggaagttctg tccattaata acaacatgct cgttatggga atcttgcggt tcgctttcca
    14221 gccaacgtaa aaaattaaag ccgttaatca attgtgcggc ggtatggttg cgcagtcgat
    14281 ccaatttttg cccaaaaagc tgacagctcg ccgggttcgc catatccact ttgcttttca
    14341 tatcgacaga gagcacaggt tcaggcaacg cctccagtaa cgcgctcaac gccagatgct
    14401 cacgttcgga aggcatccac gggacagtac gcacatcggt aacacctgca atacggcgta
    14461 tttcggccat cagactgctg aaactctcaa actccagttc agcaaaattg aggtagattc
    14521 gcccaatggg atcaatctca ataccgcgta aatcaatgcc tcttagcacg agtagatcga
    14581 gtaattcgcg ggtcagaccg agtcggtctt cacaaaagac ttccagacgc atgggaacct
    14641 tcacctgaaa aaagaacaat taatatgatg atatgacact atttgaatag caggaaggtt
    14701 tctgtcaaca atcattgaca caaagacgga aaaacgggca cttcggcgta aagatatccg
    14761 gtcagcaccc gatattgcat tatttttcgc tgggcgtttt gcctttttgc agcgtttctt
    14821 tcacctgacc gataagctga cgacggaaat ccccgaggcg aggtttgtca tcgtcaatcc
    14881 acggcagcgg gcggcaaagc tccatagctt taatccccag tcgtgccgtc agaagtcctg
    14941 caccaatccc ctgagctgcg cgggtagaca aacgagcagc gagatcttgc gacatccagt
    15001 ccattcccac ttcgcgcacc aattcgctgg ctccggcgaa agcgatattc agcaatacca
    15061 gcttgaacag gcgcaaacgg ctgtaatatc ccagttcaat gccatacagc gtggcgatgc
    15121 gattaatcaa acgcagattg cgccaggcga taaatgccat atccaccagc gccagcgggc
    15181 tgaccgcaat catcaacgtt gattcagctg ctgagcggct gatttcgcgc cgcgcctggg
    15241 catctaaaac cggctggacc agatgagcat acaggctgac cacttcacga tcgttctgcg
    15301 tttcatggat tgaggcatac cagcgttgca gcgctggatg agactgatca atacccgcct
    15361 gctgcgccag tttttcgcaa aatgcgcggc ctttgcccgt gccgtggctg tgcaacaaat
    15421 cgcgcgcttc gtcgcgttca tgggcgcgct gtcgcaagcg ccataagcgc cgccactctg
    15481 ttaccacaga acctacgcca gcgccgataa tcaatgcccc agcagcacat ccacccagcg
    15541 ccacccagtc ctgagtttgc caggcattca ttgtccactg tacaccctgc ccgacaacgc
    15601 ttgcgccaaa cagagccagc ccgcccatca ccattttgcg ccacaggctg cgtttcggac
    15661 gtaacgctgc gtccattacc gcttcgactt gcccctcttc ttcctgcgct tcgtcgagcg
    15721 tggccggggc aaaattttgc gcctgatttt cgtcaaaggt ctgctgcgcc ctgaattttg
    15781 gattctgttc gacctccagc ggaccgtcga aatcaatacg tggttttaac ggttcggtca
    15841 tcgcaattta tctcctatta aaaattccag cgcggcatca agacgaatat gcggcagcgg
    15901 tttgtcgaca tccatcacct gcgggcgaaa cgcttcaaac tggaaccctt gcttatccca
    15961 gaacgcctga ccaggcaaac gtgcgggaac ttcgccagga taaacagtga gcggtgcgcc
    16021 atcgctaagt cgattaccgc gcagcgccgg gattttctca ccgttaacat caataatgcc
    16081 actggtggtc gcctgaactg acgccagccc caggcaatcc atgctgatcc cttcgaacgc
    16141 cgcattttgc caggcatcct gaattagttg ttgcagcaat gaaaccatat tggcgtgctg
    16201 atcgatggtc acatggtccg ctttagtggc agcaaacaat agcttatcga taaccggcga
    16261 aaacaaacgc ctgaacaggg tacgctgccc atagtggaaa ctttgcatca gctgcgtcag
    16321 cgccagacgc atatcattaa atgcctgtgg cccactgttg agaggttgca ggcaatccac
    16381 cagcacaatc tggcggtcaa agcgcagaaa atgattctta tagaacccct tcaccacctt
    16441 ctcgcagtaa taattaaacc gctcgcgcag cattccggca ttggtgtgct tatcggcctg
    16501 cgccagtttg gactcgcccc aggcatcgac atccggccac gggaagaatt gcagcgcggg
    16561 cgcacctgcc atatcccccg gcaagacaaa gcgcccaggc tgaataaagt gcagcccctg
    16621 ctgtttacag tggtggagat aatcggtcca cgcggcggca atgtctgcca gccggttttc
    16681 gtcggcaggc gctagcgggt ccagcccttc gcacatcatt cgccatttcg ccgaccattc
    16741 gccgcgctga ccattgagta agcccgtcat ctggcgcgac cagcttaaat agtcctgcgc
    16801 cagcatcggc aggtcgagca accattcgcc agggtaatcc acaatctcca gatacagcgt
    16861 ggaggtatcc ttaaagtggc gcagcagcga atcgttcgat ttatagcgta gtgccaggcg
    16921 aatttcgctg acgccgcgcg ttggcgtcgg ccaggcggga ggatcgccat acagctgcgc
    16981 caacccttcg tcgtaggtaa aacgcggaat gccaaagtca cgctgaggaa tgcgttttac
    17041 gcccagcaga cgctcttcac gcaccgcact taatagcggc aaacgtgctc cggcatgaat
    17101 attgagcaac tgattgacca tcgcagtgat aaacgccgtt ttgccgctgc ggctaagtcc
    17161 ggttacggcg aggcgcagat gtctgtcgac accccgattc accagcgcat taagttcatt
    17221 tttaagtcgc ttcattgctg tcctgtgctg ctctgaagac cagaaaaaaa tcattcataa
    17281 ggatatgaat gtgggggcca ggccattgat tatcaactac ccgtgttgtc atcgacgggc
    17341 gaactccata agatgtgcag cattcacgtt cggacggagt tcctgaccta tgtcccctac
    17401 tatttatgat attgccaggg ttgcaggcgt atcaaaatcc accgtatcac gcgtgctgaa
    17461 taagcaaacc aatatctccc cggaagcgcg cgaaaaagtg ttacgggcca ttgaagaatt
    17521 acagtatcaa ccaaacaagc tggcccgcgc gctgacctct tcgggttttg atgccattat
    17581 ggtgatttct acccgttcga ccaaaactac ggcgggtaat ccgtttttct ctgaagtttt
    17641 gcatgccatc accgccaaag ctgaagaaga aggtttcgac gtgatattgc agacgtcgca
    17701 caccccggca gaagacttac aaaaatgtga aagcaaaatt aagcagaaaa tgattaaagg
    17761 cattattatg ctaagttcgc cagcggatga gtcatttttt gcccaacttg ataaatacga
    17821 tattcctgtg gtggtgattg gcaaagttga aggtcaatat gcccatgttt attctgtcga
    17881 taccgataat tttggtgaca gcattgcgtt gaccgatgcg ttaattgaaa gtggacatca
    17941 aaatattgcc tgtctgcatg caccgcttga tgtccatgtt tcagtggatc gggtaaatgg
    18001 ttataagcag agtctggaag atcataatat tgcagtgcgt gatgaatgga ttgttgatgg
    18061 cggttatacc catgaaacag cactgcaagc agcacggcaa ttattaagtc agtcaccgtt
    18121 gcctgaggca gtgtttgcca ctgacagcct gaaattaatg agcatttatc gtgcggcggc
    18181 agagaaaaac attgctattc cgcagcagtt agcggtggtg ggttatagca atgaaacgct
    18241 gtcatttatt ttaacgcctg caccgggcgg catcgatgtt ccgacgcagg agttaggtcg
    18301 acaaagctgt gagttattat tccgcttaat tgccggaaaa ccgtcaccac aaaatattac
    18361 cgttgccacg catatgacgc tgaaataaat accagcgacc ttgcctgaat gccggatgcg
    18421 atgctggcgc atcttatccg gcctacattt atagatcgga taaagtactc gcgccgcatc
    18481 cggcaattta gcccattatc agaacgagta atttacgccg acacctgcat aatggaattt
    18541 atcgctgtcg ccttcgtcgt gatcctgcca ctcaaacgcg tattccagcg aaatggaaag
    18601 accattctgg aaatcataac cgtaaaataa accaacacgg ttaaaatcat ggccttcacg
    18661 ttcaatatca tcctgccagt cccagttact ccagcgatcc agcccaatgc gcgtatacgg
    18721 cgtcaccgag tggttgccga gcgtcagcgg caaataggcg cgaatttctt gcgtggaaaa
    18781 ctcaccgtta ttacggctgt cgtccatatt gaagccgcgc tcgagataat agttcactcg
    18841 caaggcaacc gtttcgttga aggtatattg cagacctgtt tccgtttcga cacgggtatc
    18901 agcgtaaccg gtcgtgttca aatcgttggc aaatttatac atcgacaacc aaccgttgaa
    18961 acgtaaatcg tcagtcagtt tcacatccca gtctggcgcg attttccagc gctgcatatt
    19021 cgccgtgtct ttacccggtt catcaacgta gtgataacca taattacgga aaccgccggt
    19081 caggccgaaa ctgaaatcat cgctttcgag gaactgataa tgtacctcca gctccgggcg
    19141 atcaaaccac gttccacgtt tacccgcgct ataatctacc ggcccttcct gataataggc
    19201 cagagaaatt ctccacggcc cgttggcggc attaaaatag acagaaggct ccgccagccc
    19261 atccatatct tcgccataac cctcgacgtt ttctatttcg tacatcgcgc cgatattaaa
    19321 gtgccagtcg ttcttttcct cggcctgtgc gcaggccatt cccgcacaca tcaccagtgc
    19381 ggtacagggt aataactttt tcattattgt tccttgtatt atcctggggg aatgccttcc
    19441 ccctgtattt agcgaattgc tatttccgtt tctgcatcaa agaaatgaca tttagtcata
    19501 tcaaaatgaa tagtgatatt ttctcctgca tgataatcat ttaacgcacc agcacggacc
    19561 actaactcgt gccccccaac cgtggtgtag agcataaatt ccgcaccggt taattctacc
    19621 acgctaattt tggcggaaat attattttcc tcttgcgcgt ccggatgaat atcttccggg
    19681 cgcattccca tcacgatggg cttatgcaaa ctttcctgtg ttttcagaac cgctaatttc
    19741 tcttcgggaa tggttaattt aagcgtttcc gtaacgaatt tatcgccatc gatcgtgccg
    19801 cgaataaaat tcatcgctgg tgatccaata aatccggcaa caaacatatt cgcgggttgg
    19861 ttataaacgg ttttcggcgc acccacttgc tgaacaatcc cgtctttcat aatcacaatc
    19921 cgcgtcgcca tggtcatcgc ttcggtctga tcgtgggtca cgtagatcat ggtggtgttc
    19981 agtttctgat gcagcttgct gatctctgcg cgcatttgta cgcgcagctt ggcatcaagg
    20041 ttagagagcg gttcatccat taaaaacacg cccgcttcgc gcacaatcgc ccgcccgagc
    20101 gccacacgct ggcgttgccc gccggaaagc gcgcccggct tgcgcttcag gtactcacgc
    20161 aggccgagaa tttgcgccgc ccagttcacc cgctcatcaa tcacctcttt ggcgattttt
    20221 tgcatcttca ggccaaacgc catgttgtcg taaaccgtca tatgcggata caacgcgtag
    20281 ttctggaaca ccattgcgat attgcgtgct ttggctggaa cgtcattcat tcgtttgccg
    20341 tcgatcaaca gatcgccgcc gctgatctcc tcaagcccgg caatcatgcg cagggtggtc
    20401 gatttaccgc agcccgacgg gccgacaaat acgatgaact ctttatcggc aatttccagg
    20461 ttgaagtcct tcaccacatg cacctggtta tcgtagattt tttgaatatg ttgtaacgaa
    20521 agctgagcca tgttgattcc tttgctatac gttttgccag aaggccgata accgcggcca
    20581 ggtcagggag tccgttgagg gcaacaataa ttgcgccccg gttaagcctg cgccgatccc
    20641 caccgagcgc ataccgctgg cgttaatggc gtcaatgccc gcctgcgcat cttcaatgcc
    20701 gatacatgcc tgcggcggca cgcccagccc ggcacaggca gcgagaaaga tttccggatc
    20761 cggtttcgag tttttaagtt gggaagcatc cgcgcagaag gtgaaaaact cgcgcagctc
    20821 cagcgccgct aaaatcgtcg gcgcattcag ggagacagaa gccagcccta ccgggatctg
    20881 ctgtgcgcgg agctctgcca gcaaattgcg aataccgggc agcacagcat taaccgtcag
    20941 ctcgcgcagt gagtggacgt aaagcagatt tttgcgatac gccagttgcg tccgctcctg
    21001 cgggttaaag tcgccctctt tgcccccgtg ttgcagaatg cgccgcagag actcatcgcg
    21061 gctgatccct tttagggatt cgttaaactg cgcatcaatg ctgatgccaa tttcagcggc
    21121 aatctgctgc cacgcctgga aatgcagatg cgcggtatcg gtgattacac catccagatc
    21181 gaaaattacc ccttgcagtt tcattcatcc tcctgatgcg tggtagcggt cccattgaag
    21241 ggcaaaataa aatcacccaa acagaaaaca gattcttcag ccacggatat aagctgcccg
    21301 ttcaaacgca gtgaaacggg cgcagaagtt cgaatcgcaa tacgctgcgc gtcgagcgtg
    21361 acctgtaatt cgcagccctg ccagaacaga gggaaagaca actgttgcca ctgctccggt
    21421 aacgccggat tgagatgtaa ttcaccgtca cgcacgctca ccccggcaaa accctgaatc
    21481 gcccccagcc agatagcgcc agttgcggca gcgtggatac catcatcaca actatgcgga
    21541 tcagcaccaa gatcgatttc agtcccctcg cgccagaact gataactttg ggtcagcagg
    21601 ccacagcgtg cggcaacaat gccgtggatt gctttactta atgacgagtc gtgaatagtg
    21661 cgcggttcat aaaattgcag attggcaaga cacgataccg ctgagaactg ctccggcagc
    21721 atgtaattga gcatcaccac atcagcttgt ttgaggatct gcatctcgtt cacttctgcg
    21781 cgtgaataat ccagcagaat ggtttgcttc cccgccgccg ctttgtattt agccagatta
    21841 atcgccggct tagccataaa cgaatcatcc tgcggcaaaa cgccatcggg ctgcgtttct
    21901 ggcatccata gctctttgag gaacatttcg gcgcgatgga taaacgcatc gtcgctacag
    21961 ccgaactggc gggcaatatt cagcgcctgt tgaacgttgt agcgggccat atagctggtg
    22021 aaggcgttat tattgacatg ttcggtatat tcgtctggcc caataacatc atgaatttcc
    22081 agacggtcgt taaccctcac cgcgcggcta atccagaact ttgcagtttc cagaagtagc
    22141 gccatgcctt catgagcaat gaaactttca tcccccgtgg tctgccagta ttgaataacc
    22201 gcccaggcga tatcggccac cagatgatgt tccgcctgcg ccgaggccac tttttgccgc
    22261 agcccggtac gaatgttaat ggcggcaaat tctggcgtct cttcttcgcc gctgcgcgcg
    22321 ctttcccacg gaaatagcgc gccctgccag ccgctgcgcc gtgctttctc ctgcgcgcct
    22381 ggcaagttgt gccagcgata acgcagtaaa cttcgggcaa ccgtcggatc gctaaacaga
    22441 tggaacggca gcaaaaatac ttctgtatcc cagaaaacgt ggcctttgta gccttcgccg
    22501 gttaagcctt ttgccgcaat actgctgcgc tcgtcgtgag ccggcgtcat gatgcgcaga
    22561 tgataaagcg cataatctaa cgcttgctga tcgtgcgcat cgccgccatt taccgtgata
    22621 cgacgtttct gccaccattg acgccagttt tctgttgatg ctgcaagaag ttggtcgtaa
    22681 ctctgctgcg cgcatatttc aagctggcga agcgacgcgc tgccccactc gtctaaaacg
    22741 gcttgcctgt catcccgcca gtcgatccac accagttttt gcaacgtcac tgtctcgcct
    22801 gcatgaagct gcgcactggt atgttgcagc aaacggcgct ctttggcggt atagcattgc
    22861 tgcacatcac cgctcacctt acagcaacag ctgatggcca catcactgcg tccatcctgg
    22921 gtggtgtaga tcccctgcat cagatgctga ccaaacaccc gcacctgggt ttcgtcgaga
    22981 tgttggcgac cgtggttggt ttgcgtggcg tcgatacctg ttgaaatcag cactgacgcg
    23041 tcggcgtcca gtggcgtaat agtgatttcc agcgcaatga gcggcagttg gtctgccgaa
    23101 acaaagcgac ggctggcgat ggtgtaacct gcgccgttgc tggtacgcca gacaacgttg
    23161 cggcgtaatt cgccactggc aaagtcaagc tcacgctgcc aggcttcgtg ggataacgag
    23221 aaaacctcac catttatggc aatctccatc cccaagatat caggcaggtt caccagttcg
    23281 ttgatttcac cttttcccgc ccgatgatac agccccgcca gatacatccc tcgcgtctgg
    23341 cgcgtgtaat cttcttcatg gctggcgcga agcccaagat agccgttccc ctgcgccatc
    23401 agcgatgcat acttgttcag ggtatgctgg ctgaaatggg gttctgataa cgttactggc
    23461 ctggtcataa ttccacacgc gtccctgttt ctgcggattg atataacgcc gccaccagtt
    23521 gctggatgat gtacccctgc tcggcgtcgg ctatcatcac gggcttgccc tgtacgtgat
    23581 tgataaaggc ttccatgctg cccaaatggc ggttgtcgtc tgccatttcc cgttgcatca
    23641 gcgtcattaa ttcaccgttg ttatcggtgt agatatgtgc tggaaacagc gtcgcaccgg
    23701 ctttatcacc acaaaagctg acgttcataa tcgactgttc gcggatgttg agcgcaaatg
    23761 acgtttccag ccacagaatg ccgccgttat gaaattcaat ggtgccaaac agcgaatctt
    23821 cgacgctgta agttgccgga tcccactcgc caaattgacc acagctcttt tgcgtgccga
    23881 tcttttgaaa gctatgcgca ttcacgcttt tcaccgcagg atagcccagc acatacatcg
    23941 cagcatccag catatgaatg ccgatgtcga tcagcgggcc accaccctgc agttctttat
    24001 tggtaaagac accccagccg ggaacgccgc agcgacgcag ggcgcgggcg gtggtaacgt
    24061 aaatctctcc caaaacgccg ttggtcacct gttcacgcag ctgttgcgta tcgagcgcaa
    24121 aacgatggtg aaagtcgtag gccagcacct tgccctgttt gcgcgcggta tcgcacattt
    24181 cccgcgcctg ttctggcgtc atggcgggcg gtttttcgca catcacatgg cagcccgctt
    24241 ccagcgccat cagggtatgt tcgtaatgaa aacggttagg tgagcagacg ctaaccacat
    24301 caggtttcac cgccagcagc atggcctgcg gatcgtccca cacggatgca ttcccgtatt
    24361 tttctgccag cgcctgcgcc tgggaaaggc ggctgtcaca gacagccacc agttccagat
    24421 cgttgcgggt gcagtagtac gaagcatgaa ccttatccgc cacctggcct gcgcctatta
    24481 tcgcgacccg cagtggagag cttgtcattg cacttttcac aaagacctct tagcaggtac
    24541 gcaaccaggt caacgaatca cggtacgcct gggcaggatc ttctgcccgg atacgccctt
    24601 catacaccac ataaccctga tagttatcgg cgcgcagctg ttcaaacagc gcgtggaaat
    24661 cgagggtgcc gctgcccggc tggtaacgat ggttatcggc aatatgcaca tgaccgagca
    24721 agtcgcggtt gtcatgcagc gcctgcgcca ggttatcttc ttcgatgttc atgtgataga
    24781 aatcgccgat aatctgcaca tgtttaagat tgttttcgac gctgtaacga cgagcatcgg
    24841 cgagggtgtt gatcatatga tcctgatagc ggtttaacgg ttcgagatac accacggttc
    24901 cggtacgcgc ggcgacctgt tccagtacgc gcagggaatc actcaccatt ttgcggtcgc
    24961 cgtccaggct acgcggcgag gtcatcggcg gtaagcggaa ggtaaacatg ccccacgctg
    25021 ccggaacgac gataccttta ccccccactt ctgccagcgc ttcgagaatg cgttcgatct
    25081 gctttaagcc attaagacga cgctcttcga taaagtcacc aatccagccg tcatagccgc
    25141 cgcaggcggt ggtcaccggt aaaccggttt ctttgatcgc cgatttgact tcttcgatgt
    25201 tgttgaccag cagtttgccg tcaatctcaa aaccatcgaa gcccatctct ttgatataac
    25261 gaaatttctc cagaatattt tccgggaaga acgcctgatt ctgtgtgcct attttcattt
    25321 tttcattcct ttgaattaaa acgtgacgcc cattttgatg ctctgttccg gatgctgatc
    25381 gacatactgc atgtagcttt ccgggctgtt ggcaaaggtc actaccgggt cgattaaatc
    25441 ttcgcaattg agataaccgt tcatcagcag tttccagcag gtttcttcaa tacgcttacg
    25501 gctccagcgc ggataatccg ggttcggttc actgcacgcg cgagagaaga caattttggc
    25561 gttattgaaa tgcgcttcgc gtccgaggtt aaaaccttca gcaaacggtt tagcaaacgc
    25621 gacataggag atggtgccac cgtaagccag gccgcgcagc gccgactgca gcgcgtcggc
    25681 gtaaccgctg gtttcgataa tcacatcggc accctgcttg ccggtcagcg ttttgatctc
    25741 tttgcctacg tcagtgccaa tgggattaag gcagaaatcc gcaccgtgac gacgagcaat
    25801 atcacaacga tgggcaatag gatcgacgcc aattaccacc gacgcgccag cgcgtttagc
    25861 cagttggatg gcaatttgac cgatcgcgcc aagccctacc accactacaa aatcccctac
    25921 gcgtacgttg gcatcgcgaa cgccactcat ggcaaactgc gccgggtcgt agcagacagc
    25981 gtttttccag gagctgcctt ccggcatttt gcgcagcttg tagttattca ctgcgttaat
    26041 gatgatcgtc tcggagagcg ggccgtagcc gcatacgctg tcgcccaccg cgtagtcggt
    26101 aacgtcgctg ccgcactcga taatgtcgcc aaccaccatg ttgccaagct ggaatttgcc
    26161 aaactcaatg ccgcgcggcg catctgccgg acgcggggtg aacatctgcc attcgccgtt
    26221 aaagtcttca tcgataaacg ggctggcggc gcggaagtcg accacttccg ttccgtgttt
    26281 cggtgcaccg aaacgcacgc ggatcttcac ttcattggct aaaatcgccc gatcttcata
    26341 ctcaaccagc gcagcaacgc gcggtgctgt ggctactaac tttttcatgt aaaactcctt
    26401 gtaaagaaac tggccgcttt agcccttcac gccaccggcg gtcaaaccac tcttaataaa
    26461 acgttccgaa agcgcataca taatcaccac cggcaatgcg gtcaccagcg aggccgccat
    26521 catccgtccc cagatgtaat ctggcgtact gaacagcgcg ttcaggccga ctggtaaggt
    26581 gaaattgctg gcgctggaga ggaaaatcga cgcaaacaga tagtcgttcc atgccaccat
    26641 aaagcagtag acgaatacgg atatcagccc ggacatcgcc aggggcacgg tgatacggaa
    26701 gataatttgc agacggttga ggccatccat catcgccgct tcttcgatct catcagggat
    26761 ggtgtcgaag taacttttca gcataaacac ggcggtgggc agagtttgcg tcaccatggt
    26821 aatgatcagc gccatctcgg tgtcgtaaat gccaagtgcg gtgataattt tgaacagcgg
    26881 caccaccagc aaaatgccgg agaacatata caccgtgtaa aagctggcgt tgatggtcat
    26941 ccggccttta aagcgcaggc gagaaagcgc gtaagctccg agaatgccga gaaataccgc
    27001 caccacggat gaaaccaccg acaccaccat gctgttacgg aagtagtcga caaacgggaa
    27061 aatcaccggg ttaaaaatgt cgacataatg ctccagcgtc cactgttgcg gcagcagcgt
    27121 aggatgcagc gagatcgcct cttttgcgct cttgaacgag gtcatcagca tcacaaaaaa
    27181 cggaaacagc gtgatgatga gaaacagcgc cagcccgcgg taaaaaccga tacgactgag
    27241 tgtgcgttta tttgttgcca ttgaggttca cccgtttcct ggtcagcaga atgacagcga
    27301 aaataatgat gaagagcacc accgagatcg ccgccgcttt ccctaagtca ttaaaggcaa
    27361 aggcggtttt gtagagatag acaccaagaa tatcgacctt ggttgtcagc agataaacat
    27421 ccgcgaacat gtagaacatc cagatggtgc gaagggtcac caccgtcgcc aggaccggca
    27481 taatcgcggg cagcgtgacg atacgaaacc gttgccaggc attagcgcca tccatctctg
    27541 ccgcttcgta cagcgatttg tcgatggtct gcaaaatcgc cagaaacgag ataaaggcat
    27601 acgggaagta gcgccagatg gcgaagatca ccaccagcac aaaactactg cccggattat
    27661 cgaaccacag cggtgcctgc tcataaagat gcagaagatc gacgccgagg tagttcacaa
    27721 tgccgtagcc gttgttgaac atgtatttcc aggcgaacac cagcgaaata gacggcgtta
    27781 cgtaggagag gatcaccagc gaacgcgcgg ttttgcgcag gcggaactcg cggttgaaaa
    27841 acatcgccac tgccagcccg agcacggtgc tgcccgccac caccaacgcg gtataccaga
    27901 ccgtcatcca cagcgagtgc cagaagccgg gatcggagag aatacgcaca tagttgctta
    27961 ccccaacaaa cgttgactcg atgttgggat tgagcggcag acgtaaaaaa ctaatttcga
    28021 tattcgacac cattggccac gcaaccagac cgcccagcag taataagctg ggtgcgagaa
    28081 gcaacagcgc aaagggcata tcggaacgac ctgaaaaaag cctgttcatt gcggattcct
    28141 gtcctgggtt tagtgctgtt cgatcagttc atccagcttt ttctggctcg tttgcagcgt
    28201 gccagggaga tcggctttac ctacggtgac gttatgcacc atggagctca ccaccccaga
    28261 ccccgtcaca tcgcccatgc gggtaaattt tttatcccct actgcgccaa agacctgaat
    28321 attcggcagt tcaccgatta actgatttgg cagttcaccc agcgccttaa taacgtcgtt
    28381 gtctttccag gtggaagtag tgacaaccgc tttgttcacc ggcagcgccg cgcctggcga
    28441 catcatcacc cagtcggcaa tgttgtctgc ctgctccata aaggtgacaa atttttcagc
    28501 cgcttccgtt tcttcagtct tttgtccggc ggtaatggtc agcgaggtca acatgccgta
    28561 gacggcagag tttttctcgg ttggcaccac gaaaccgacg tttttcggat cgccttcttt
    28621 aatcacagcc ggaaggatat aggtggagta aatcgccatc ggcgcggtgc cgttcataaa
    28681 ggcgtctttc acttccatga tgtcgttaga acccggcatg gtgttggcag caaggtcgcg
    28741 gtaataggtc agcgcctgca tcatctctgg tgaatcaagg gtgattttgc cttcggcgtt
    28801 aaagacgttg gcctagttgg ataacgcaaa ctgggagaag gattgttccg tcagcacgct
    28861 ttctgccgta ggcagcgcaa taccgtattt tttattcgcc gggtcattca gtttctgtgc
    28921 aacgtccagc agctgttgcc agtttttcgg ctcctccagc cccgcttttg ccagcacatc
    28981 tttgcgatac cagatgccgc caatccaggc gctgacagga acaccggtcc atgcgctacc
    29041 atcttcggta cgcacaatac gcagtacgcc atcgtaaaac gcgccttcac caacgttgct
    29101 gatgaccgtg gcaaccgcct tgcgatcgat aagctgctct ttgtccataa ctttggcgta
    29161 gtcatgactg gtttcaatca cttccggcag cgagccgcta cgtgaaagag tgatgacttt
    29221 ggtgttataa gcgtcctctt cgacgggcac ttgtttaacg gtgatacctg ggttttcttt
    29281 ttcaaaacgg gcaatcaatt tgctgataac tgcctgacgc tcctgctcca ccgaagaatg
    29341 cataaattcg atggatacat ggggtttatt ttcttcttta cagcctgaaa tcagggcgca
    29401 ggaaaccagt gctgataaca gcacgatttt tgatttaatc atgagatgtt cctttttatt
    29461 taatccacat aacctgccag gggcgcagcg ttattttatt acccaatata tcaacttcac
    29521 taatcagatc ccgaccgtgc aggccagtaa tatttacatg ctgaatattt ttactgacat
    29581 taaacaatcc ggtcagacaa ttaccctctg cgttacttct ttgaatacgc attacggatg
    29641 aattaaccgt atcgatggta aaattattat ccggatgaaa ctcgttatgg ctgcgacgaa
    29701 gtgtaattaa acgcgacaac tcatgatata ccgcatgcct taatgtagct tcatcgttca
    29761 gttctcgggt tatctcttca ctgtgatatt ttttacggtt aatcgcacgg ttatatccga
    29821 gtttttcgac acctgcgtaa tcattacgcg agcccagaat actttgaata tatatcgctg
    29881 gcacaccggg gaaacttaac aaaatcgcat gggcaaggat aaacctggcg caacgttctt
    29941 catcgctact ctcgcggcgg cttaacgcat ccatataggt gacatttatt tcatagggac
    30001 tgcgcgtacc gtcgggatta tttttccagt ttactaatgc accttcctgc tgtaatgcct
    30061 cgaccagcgc taatatttcg ctttctggta acaagccccg cagtgggttc agcccgatgc
    30121 catcgtgaga ggcgaggaag ttaaaccagg tcgttttacc ggagggcaag ctcagacttt
    30181 gcgcccactg gcacagcgcc tcgacgttct gtttttgcac cgcatgtaac accagcggcg
    30241 gcagcgagaa ctggtacacc atatgcgctt cgtcatcgcc ttcgccaaag taagcaatgt
    30301 tatctttatg cggaacattc gtctcagtaa taatcaccgt acctggcgcg atgtcatcaa
    30361 taatcgaccg taacagtttg ataatcagat gtgttttttc cagatggatg cagcttgttc
    30421 ccggctcttt ccacataaag ccaacggcat ccagacggac atattctgcg cccttttcca
    30481 ggtaacaaag caaaacatcc accatcgcca gcaacacgtc agggctacgg taattcaggt
    30541 caatttgatc gtcgctaaag gtggtccata aatggcgcgt ggaattatca tccatttgaa
    30601 acggcgttaa tagcggtaat gcacgcggac gggtgacagc gctgagatcg gtttgcggat
    30661 caaccgcaat aaaaaaatct tcaaaacctg gctgctgttg taaatagttt ttaaaccatt
    30721 cactttttgc cgacatatgg ttgcagacaa aatcaaacat taaatgactg cattcaccga
    30781 gctgctgaat atcctgccac tacccgcttc actggcgacc tgatgataat caattaccga
    30841 aaatccatca tcagatgacc atggataaaa aggtaataaa tgaacatgtg aaaaaatgct
    30901 ttgcagccat tgatggtaaa actgattaaa tgtgggtagt ggttttaaat cattactgtg
    30961 aaattgatcg gcataggtaa ttaacacgac atcactttca tcccagtgtt ttttacgtcg
    31021 ctgtgtaatt aatcgtttcg cactctcaag acgcgttaca agtttctgta aatgagttgc
    31081 ggtaaatgtt ccaccgtaga tttcgtccag gtaatccgta attttctgtt tcatgttgcc
    31141 tttcctggta gcggtcccat gaaatgagag actactccgc agaaaataaa ctgtcaattg
    31201 agcacaaggg ttttagtcac gcaggaaatc attttttacc agggaaaaag cgtatttgtg
    31261 agctgacgca aataatctat agggatgtaa ataaggtgtg acagaaaaaa acggcgcata
    31321 agcgccgctc atggtgaatt cttttaacct ttgaccttcg gcattgcgat gtctttcagg
    31381 ccaccggcgt tctcaacgtg ggtatatccc atctcgctaa ggatctcttt tgcctgccct
    31441 gattggcgtc cggcattgca atacactttc acggtgtcgt ttttatccgg aacgacggtg
    31501 gcaatgcgct ctttcacttc cttcagggga atattgatgg ccccctgaac gtgctcttgc
    31561 tgatactgct ctggaacacg aacatcgatc cagtgttcag cggcgaaaac gggcagtgaa
    31621 aacaccagtg ccagagcaag taagcctttt ttaaacatgt aagcgtcctt ctatggatga
    31681 atattgctat ccattatacg cctgagattt aacaattttt gcgaaactcc cctcaccttt
    31741 tataacgctg cgccagttta ttggcagccc gacttaacag cggttccagc gccacagcca
    31801 gcaacatttt cagcggtcgg cgagcaactg attttatcgc ccagcccgcc acacccgctg
    31861 ggccatagcg cagtgcggta agaagtacca gcttgcctgc taatttgaaa ccaggcttta
    31921 ccttttgccc ggcctgttgc cagcgagtat tcataacttt cctcacagtt gacggaaacg
    31981 gctacgtaac gtgaatgtat cggaagtgac ataacgttcc atctcgcgta aacgcgtttc
    32041 acttgccgcc agttcacggt cgacttcatc cagcaattca ctgctggaag gtagctgctc
    32101 accaaaggcc atgttgtccg gcattggatc aagcgcaaat gacaaaatga tgtaagcaac
    32161 cagggtaaac agcgccagac cgaagaaaat cgacaacacc accaggatac gcaccagttt
    32221 taccggtaca tcaaaatagt tggcaatccc ggcgcagacg ccacggacca tgccctgctg
    32281 tggaatacgc cataattttt tattgagatt aatgcccgcc attagcgatc cctccagttc
    32341 ggatgttctg catcaagaat agattccagc gcctgaatac gttcgcgcat ccgttttgct
    32401 tcatcagcca gttgcgctaa tcgctgctgc tcactttgcg acaattcact gcgaccagaa
    32461 cgattgctgt aatgcagcca taaccagatc ggtaaaacaa acagcacaaa aatggttaat
    32521 ggaatagcca gaaatagcgc gctcataagt actccttaga cgatgagcgg cggcgcgttc
    32581 ggacgccgcc tggatgttat tattgattgt cttgcttcat tttggctttt aattgtgcca
    32641 gttgctcgct gattgcatca tcggctttca gttcggcaaa ctgatcgtcc agcgattttt
    32701 gtttaccgaa gctgtggctt tctgcttccg cttccatctg gtcaatacga cgttcgaaag
    32761 attcgaaacg agccattgct tcatccagtt tgccactgtc cagctgacga cgcacatcgc
    32821 gcgacgagtt tgccgcctga tggcgtaaca tcaatgcctg ctggcgagcg cgtgtttcgc
    32881 tcaatttgtt ttccagctcg ccaatctctt tcttcatgcg tgccagcgta tcgtccacca
    32941 gcgtcacttc atgttccagg gacttaatca gatcggtcag tttctgtttt tcaattaacg
    33001 ccgcacgcgc caaatcctct ctctctttca gcagcgccag ttcggctttt tcctgccatt
    33061 caacttcacg cgccgacgct tgttcaatac ggcgagtcag ctgtttcttt tctgccagcg
    33121 cacgcgccga agtagaacgt acttcaacca atgtatcttc catctcctgg atcatcagac
    33181 gcaccagttt ctgtggatct tccgctttct ctaacagagc gttgatgttg gcattcacga
    33241 tgtcggcaaa gcgagaaaaa atacccataa tgttgtcctc ttgatttctg cattcaggat
    33301 tgtcctgctg aactgttaat acaatttgcg tgccaatttt ttatcttttt gatttataaa
    33361 gatctgattg aagaatcaac agcaacatgc caggatgaat tagctaatta cactaacaag
    33421 tggcgaattt catcatggca gaatacaaag ataatttact cggtgaggcg aacagctttc
    33481 tcgaagtgct ggaacaggtt tcgcatctcg caccgctgga caaaccggtg ctcatcatcg
    33541 gcgaacgcgg caccggtaaa gagctgatcg ccagccgcct gcattatctc tcctcccgtt
    33601 ggcaagggcc gtttatttcc cttaactgcg cggcgttaaa tgaaaatctg ctggattccg
    33661 aactgtttgg tcacgaagcg ggggcgttta ccggtgcgca aaaacgtcat ccagggagat
    33721 ttgaacgtgc cgatggcggt acgctatttc ttgatgaact cgctacggca ccgatgatgg
    33781 tgcaggagaa attattgcgc gtgattgagt acggtgaact ggagcgcgtt ggcggcagcc
    33841 agccgttgca ggtgaatgtg cggttggtat gcgcgacgaa tgccgatctc ccggcgatgg
    33901 tcaatgaagg tacttttcgc gctgacctgc tcgaccggct ggctttcgat gtagtgcaac
    33961 tgccgccact acgcgaacgt gaaagcgaca ttatgttaat ggcggaacac tttgccatcc
    34021 agatgtgtcg ggaaatcaag ctgcctctgt tcccggggtt tacggagcgc gccagagaaa
    34081 cattgctgaa ttatcgttgg ccaggaaata ttcgtgaatt gaaaaacgtg gtggaacgtt
    34141 cagtgtatcg ccacggcacc agcgattatc cgcttgatga catcattatt gatcccttta
    34201 aacggcgtcc gcctgaagac gctatcgccg tttcagaaac cacctcgctt ccaacactgc
    34261 cgctggattt acgtgagttt cagatgcagc aggaaaaaga gttgctgcaa ctcagtttgc
    34321 aacaagggaa atataaccag aagcgcgcag ctgaattgct gggcttaacc tatcatcagt
    34381 tccgcgcgtt gttgaaaaag caccagattt agcgaagttg ggcaatacaa cttgccggat
    34441 gcggcgtgaa cgccttatcc ggcatacata atcaatacgt cgtttgcagg tctgataagc
    34501 gcagcatatc aggcattatt gcgttgcccc tgcttctgcg tattaatctc tcagcgcatc
    34561 ctgcaaaatt ttcattgccg catcgaattg cgcatccggg atggtcagcg gatacaggaa
    34621 acgaatcacg ttgccgtatg cgccacaggt cagtagaagc agtccctgcg ccagcgcgcg
    34681 ttgctgaatt ttttgcgcaa tggcggctga cggctcgccc gtttgcgggt cgttaaattc
    34741 tgccgcaatc atcgagccca gaccacgtac cgccgcgatg gccggaacgt tttctttggc
    34801 atcaatcagg gttttcgtca gacgctgtcc caactgcatg gcgcgctcac agagtgactc
    34861 tttgtcgata atgttgagca ccgcgtgcgc ggcagccacc gccagcggat tcccggcgta
    34921 ggtgccgcca agcccgcccg gcgcgggcgc gtccataata ttcgcgttac cgaccacacc
    34981 cgaaagcggc atcccgcccg cgaggctttt cgccatcgtc attaagtccg gcttatcggc
    35041 gtaatgatcc atcgcaaaca gtttgccggt acgcgcaaag ccgctttgca cttcatcggc
    35101 aatcatcaca ataccgtgct cgtcgcacag gcggcgaatg gcggcaacca gctcttttgg
    35161 cgcaacgttg aaaccgccct cgccctgcac cggttcgaaa ataatggccg cgacctgctt
    35221 cgcttcgatg tctgatttaa acaagcgttc gatggcgtcg agggagtcct gtgttgaaat
    35281 gccgtgtaaa tctgacggat aaggtacgtg atacaccgaa ccagggaacg ggccgaagcc
    35341 gattttgtac ggcgcgacct ttccagttaa cgccatagtc atatacgtac gaccgtggaa
    35401 gccgccgcta aacgcaatca cgccagggcg tccggtatgg gcgcgggcga ttttcaccgc
    35461 gttttccacc gcttccgcac cggtggtgaa gaacgcggtt ttggcctgcc cgctcaccgg
    35521 ggcaagggcg ttgattttct ccgccagggt gacgtagctt tcatatggca caatctgata
    35581 cgcggtgtgg gtaaactgtt gcagctgctg ctccaccgcc gcgaccagat cagggtggcg
    35641 atgtccggta ttcagcaccg caatgcctgc ggcgaaatcg atgtactcgt tgccctcaac
    35701 atccttcagc gtggcgtttt cagccgactg ggcgaagaag ttacacatca cgccaacccc
    35761 gcgcggggtg gcagaaagac gacgctgatg gaattcattg ttgctcatgt ttcacacctt
    35821 ttgactcttc actgttaatc ggaaattaaa cgcccagacg atcgcgcagg ctgtaatacg
    35881 ccgcgcccat cgcggtaaac ggtacgcgca gcgtgcgccc gccggggaac gggtaatgcg
    35941 gcagattggc gaaggcatcg aaacgttcgg cgtcgccgcg cagcagttcg gcaatcaaac
    36001 gtccggcgag atgagtacag gtgacgccgt ggccgctgta accctgcatg taatagatgt
    36061 tggtatcgag gcgaccgaac tgcggcatac gcgacagggt cagcaggaag ttgcccgtcc
    36121 agcgatagtc gattttcacg cccttcaact gcgggaaggt tttcagcagt ttcggcacca
    36181 caagacgctc aacgtcatcc gggtcgcgcg cgccgtacac cacgccgccg ccgtacagca
    36241 aacggttgtc ggcggtaaga cggtagtaat caagcagata attacagtct tccacgccgt
    36301 agtttttcgg gatcagcgaa cgggctaaat catcggaaag ccgctcggtg gtgatcacct
    36361 gcgtaccgca cggcatgctg cgtttcgcca gttccggctc cactttatcg cccaggtacg
    36421 cattcccggc gacaatcaca tacttcgccg tcacctgacc tgtggctgta cgcactacgg
    36481 cgggcgtggt gtgctggatc tgcgtcactg cggaaagctc atacacgcgc ccgccgttca
    36541 ggcggatagc gtccgcttcg ccaatggcga ggttcagcgg gtgaatatgc ccgccgctgt
    36601 gatccagcag cgcaccagtg tagcgatcgc tggcgacctc gcggcgaatg gcgttggcgt
    36661 ccagcatctc cagctgttta ttgccgtagc gttcccagtt ctctttctgc tcttcaagtg
    36721 tggcgagctg tttatcattc atcgccacaa acagaccgcc ggggcggtag tcgcagtcaa
    36781 tctgataacg tttgatacgt tcgcggatga tctcaccacc ttcgaacatc atgctgccga
    36841 gcatacgggc ggtgtccatg ccgtagcttt tttcgataac gtcgatgtcg cggctgtagg
    36901 agttcacaag ctgcccgccg ttgcgcccgc tggcgccaaa gccgatgcgt gaggcttcca
    36961 gaaccaccac gtcaaagccc gcttccgcca gatgcagtgc ggaagacagc ccggtatagc
    37021 cgccgccaac cacgcaaacg tcgcaggaaa ttgactcatt cagcgtgtcg aatggcgcgt
    37081 atttattcgc actggcggcg tagtaactgc tggtatgttc tgtcatttca ggcctccagg
    37141 cttatccaga tggttttcag ttcggtgaat ttttcaaggg catgcaggga tttgtcgcga
    37201 ccgttgccgc tctgcttata gccgccaaac ggcacggtca tatcgccgtc gttgtagtta
    37261 ttgacgaaga cggaaccggc tttcaggcgt cggctcatgc ggtgcgcgcg ggagaggtcg
    37321 cgcgtccata ccgccgcgcc aaggccgtac tggctgtcgt tggcaagctg tagcgcctgt
    37381 tcttctgatg tgaaacgcgt gaccaccagc accggaccga aaatctcttc gcggcttaag
    37441 gacgcattcg ggtctacatc cacaatgatg gtcgggccga tggcgacagc cagcccggcg
    37501 ttacggccat ccaacaacag ttgccctttg ctttcgcctt cctgaataaa gctatggacc
    37561 gagtcggcgt gggcgcagtc gattaaggtg cccatggtgg ttgcgggatc aagtggatgg
    37621 cccggctgcc agttttgcgc ctgctgtttt aacagggcta agaattcatc ggctatgctc
    37681 tcttccagca acaggcgcgt tccggcgatg cacacctgcc cctggttgta gaagatgcct
    37741 gctgcggtgg cgcttgccgc ctgttgcaaa tccgggcagt cagcgaaaac gatgttggcg
    37801 cttttgccgc ccgcttccag ccagacgcgt ttcatgttgc tgtcgcctgc atctttcagc
    37861 agctgtttcc cggtacgggt cgaaccggta aaggcaatgg cgtcgatatc gttatgacgc
    37921 gacagcgcct gccccgcttc atgaccaaaa cccgtcacca cgttcaacac accatccggc
    37981 aagcctgctt ctttcgccag ccccgcgaga cgaatcgcac tgagcggtga tttttcagac
    38041 ggtttcagga caacgctgtt ccctgctgcc agcgccgggc caagcttcca gcaagtcagc
    38101 agcagcggga agttccacgg cacgatggcg gcaatcacgc cgaccggttc acgcacgatc
    38161 atcgccagct catggctact ggtggtcgcc acttcgccat acactttgtc gatcgcttcg
    38221 gcgtaccagc gaatggcgcg cgccgcgccg ggtatatcat cacgcagact gtgacgaatc
    38281 ggtttgccgg tgtcgagagt ttccagcagt gccagctctt cggcgttggc ttctattaaa
    38341 tcggcgagtt tattcagcac cgctttacgt tttgccggag aagagagtga ccagtcgccg
    38401 cgttcaaata cgccgcgtgc tgcgctcacc gcacggtcga tatcgacgct cttgccgcgc
    38461 gcaatattcg ccagcggtgc ctgggtaacc ggatcaacgg tttcaaaggt ttcattttcc
    38521 gccgcagcag tatattcacc gttaataaat aagcggtttt caatggcgag acttaacgct
    38581 ttatcctgcc agtaagccag atgatgaaaa ttcattatga ctcctgtttc acgtctatca
    38641 gatatatgca gtttgtgaac gtcagggcgc agagatcccc gggccgtatg cccgtttatt
    38701 tctggcgaca ttctttatgg attgccgggc gcattgcgct tacccggctt acagaacaaa
    38761 aagattaaaa cgtggtgggc gtatgggcgc tgataattcg gcaaatacct gccgacgtat
    38821 tactgaaact gtgcgggatg ccggtattaa tggcataact ttgccccgcg acgaggtggt
    38881 aatcctgacc attaatcgtc agaataattt caccttccag tacagtgcct atttcctcac
    38941 cctgatgctt aattctttcc ccagtggttg tgcccggctg gtacgtttca aagatcatcg
    39001 ccagcgtgcg attcgggtta ccgttatgaa ccagcttcat tgacacaccc tgactgccca
    39061 tctcaattaa atcgtcctga ttaatgacga cctgcggctc atcaggtttt tccggctcgg
    39121 aaaagaattc cgagagtgac agaccataca ccttcagcag cttttgcagc gtactgatgg
    39181 cagggctgac tttatcttgt tctatcgtac tgatagcact gtgagtcagc ccggagagtt
    39241 cggcggcacg acgttgtgaa agcccctgct gctggcggat ttccgacaag cgttttcctg
    39301 gcgccagtcc ctcatcactc atatttgcat ttccttaaac tgtagtggtc agagtcgctg
    39361 tttttcagcg atatggtgct gacaagcggt gataaagccc tcgaacaata tacgcgaaag
    39421 cgcgtactcg ctactgttcc attccgggtg ccactgtacg cccagcgcaa aaggatgatt
    39481 gatgacacta accgcctcaa ctaagccatc cggcgagcga gcttcaacac gcaaccgtgg
    39541 gctaacgacc ttcgcccctt gtccatgcag agagtttacc caaaagttgc tacattcagg
    39601 taacaatgca gacagcaatc ccccctcttc aacctgaact tcatgcgacg gtgcatattg
    39661 ctgttccacc ggcagttcgg gatcttcccg atgttccagc aattcaggct gttcgcacag
    39721 cttgcgatgc aacgaaccac cggttgccac caccagttcc tgtaaacccc ggcagatggc
    39781 gaaaatgggg atgcgccttt cgagtgcggc attaattaac gccatgctca gaagatcacg
    39841 ctcgggatcg gcgtcaggct catcgccgtt ttcaccatat aggtgcggct gcacattgct
    39901 gggactacca ggaaggtaaa tgccatcgag tttcggcaaa agttgttcaa gtaacgacgg
    39961 ttccgccagc gcatgtggca gcgcaattgg caagccgcct gcgtggatga tggcattcag
    40021 gtacttttct tgcagagtct gggtcgcatg ccccttaagc ctgttcctgc acattacgac
    40081 accgataacc ggattgttca ttatattttc cattctcaat ctcacaaagt ggactaaatt
    40141 atcgccatta ctgcggttta aatgaccttt atgttcaata ttttttcaat ctagcagtgg
    40201 gttttcattt ttgcaaactc aatttaacat ttgacaaaca tttagtttgc atacagattc
    40261 gaatggtggt cattatattt tacgctttga taacgagcgg aaaacaaacc aaaggcgaag
    40321 aatcatggaa accaatatcg ttgaagtaga gaactttgtt cagcagtcag aagagaggcg
    40381 gggtagcgcc tttacgcagg aagtgaaacg ctacctggag cgctacccga atacgcaata
    40441 tgttgatgtt ttacttaccg atttgaatgg ttgcttccgt ggaaaacgta ttcctgtttc
    40501 aagcctgaag aagctcgaga aagggtgtta tttcccggcc tcggtatttg caatggatat
    40561 tctgggcaac gtagtagaag aagcaggtct gggtcaggaa atgggcgagc cggatcgtac
    40621 ctgtgttcct gttctcggtt ccttaacccc ttctgccgcc gatccagagt ttatcggtca
    40681 gatgctcctg accatggtcg atgaagatgg cgctcccttt gacgttgagc cgcggaacgt
    40741 tcttaaccgc ctgtggcagc agctgcgcca gcgcggattg ttcccggtcg tagcggtaga
    40801 gctggagttc tatttactgg atcgccagcg cgacgctgaa gggtatctgc aaccgccctg
    40861 cgcgcccggc actgatgacc gcaatacaca aagccaggtt tactccgttg ataacctcaa
    40921 ccacttcgcc gacgtgctca atgatattga tgaactggcg cagttacagc tgattccggc
    40981 agatggcgcg gtcgctgagg cctcgccggg tcagtttgaa atcaaccttt accatactga
    41041 taacgtgctg gaagcctgcg atgatgcgct ggcactaaaa cgcctggtgc gtctgatggc
    41101 agaaaagcat aagatgcacg ccacttttat ggcgaagccg tatgaagagc acgcgggcag
    41161 cggaatgcat atccatatca gtatgcaaaa taatcgtggc gagaacgtgc tttctgacac
    41221 agaaggcgaa gattcgccgc tgctgaaaaa gatgctcgcc gggatgattg acctgatgcc
    41281 gtcatcaatg gcgttgctgg caccaaacgt gaactcgtat cgccgcttcc agccggggat
    41341 gtatgtaccg acgcaggcgt cgtggggcca taataaccgt accgtcgccc tgcgtattcc
    41401 atgcggcgat cgtcataatc accgcgtgga atatcgcgtg gcgggtgccg atgccaaccc
    41461 atatctggtg atggcagcga tttttgccgg tattttgcat ggccttgata acgagctgcc
    41521 gttgcaggaa gaagtcgaag gtaacgggct ggaacaggaa ggcttaccct tcccgattcg
    41581 ccagagcgat gccctgggtg agtttatcga gaatgatcac ctgcgccgct atttaggcga
    41641 acggttttgc catgtgtatc acgcctgtaa aaatgatgag ctgttgcagt ttgagcgcct
    41701 catcacagaa acagaaattg agtggatgtt gaaaaacgca tagtacccct tgcggggttg
    41761 tttcatcggc ctgagcggag ccaggcctga catttgctgg taatccagac atttcccgga
    41821 tgtcgcgtta tggagcgcag gcagcaacgg gcttccgtta acgattaaga ctgtgacgag
    41881 gaaatgagat gatgccgatg attaaatccc cgcatggcgc gggaggctgc gtatgcgccc
    41941 ctcctgcgac tgactggact ccgccccctt tgcttccctt gctaaacagg tttgatttca
    42001 ggtcgacacg accgcaaacc ttattacgca ggggaggcag caattatggc tattaattca
    42061 ccactgaata ttgctgcgca acccggcaaa acccgtctgc gaaaatcact gaaattgtgg
    42121 caggttgtga tgatgggtct ggcctatctc acgccgatga ctgtgttcga tacctttggc
    42181 attgtatctg gcattagcga cggtcacgtc cctgcgtcct atttgctggc gctggcgggc
    42241 gtgctgttta ccgctatcag ctacggcaaa ctggttcgcc agtttccgga ggccggttcg
    42301 gcctatacct acgcgcaaaa gtcgattaac ccgcacgtcg gatttatggt cggctggtca
    42361 tcactgctgg attatctctt tttgccgatg atcaacgttt tgttggcgaa aatctatctc
    42421 tccgccctct tcccggaagt gccgccgtgg gtgtgggtgg taaccttcgt cgccatttta
    42481 accgccgcga atctgaagag cgtcaacctg gtcgctaact tcaataccct gtttgtactg
    42541 gtgcaaatct ccatcatggt ggtgtttatc ttcctggtgg ttcagggact gcataaagga
    42601 gaaggcgttg gcaccgtctg gtcacttcag ccgtttatca gcgagaacgc gcacctgatc
    42661 ccgattatta ccggggcgac gattgtctgt ttctcgttcc tcggtttcga tgcggtgacc
    42721 acactttcgg aagagacacc agacgccgca cgcgtgatcc cgaaagccat cttcctgacg
    42781 gcggtctatg gtggcgttat ctttatcgcg gcgtcgttct ttatgcagct gttcttcccc
    42841 gatatcagcc gctttaaaga cccggacgcc gcactgcctg aaattgcact ctacgtcggc
    42901 ggtaagctgt tccagtcgat tttcctctgc accacgtttg tgaacacgtt agcgtctggc
    42961 ctggcctcac atgccagcgt gtcacgtctg ctgtatgtga tggggcgcga caatgtgttt
    43021 ccggagcgcg tgtttggcta tgtgcaccca aaatggcgga ctccggcact gaacgtcatt
    43081 atggtcggaa ttgtcgcgat gtcggcattg ttctttgatt tagtcaccgc gacagcattg
    43141 attaacttcg gtgctctggt ggcgtttacc ttcgtgaatc tgtcggtgtt taatcatttc
    43201 tggcggcgta aaggaatgaa taaaagctgg aaggatcact tccactattt gctgatgccg
    43261 ctggttggcg cgctgacggt gggtgtgctg tgggttaacc tcgagtcaac gtcactgaca
    43321 ctcggtctgg tatgggcttc gttaggcggc gcatatttgt ggtatttgat ccgccgctat
    43381 cgcaaagtgc cgctgtacga aggtgacaga acgccagtga gcgaaacgta atagtcggat
    43441 gcgcgccgca ttatcctgcc tgagggtaat gcgccgcgcc attaactata aaaagatgta
    43501 tgacccggat cacgagtgct atcatccgcc cttatgctta gcgcattaac cccaggggca
    43561 aataatgaac cacgatatcc cgctaaaata ttttgatatt gccgatgagt acgcgaccga
    43621 atgcgcagag cctgtcgcag acgctgaacg cacaccgctg gcccactact tccagttact
    43681 gctcacccgt ttgatgaaca atgaagagat cagcgaagaa gcccagcatg aaatggctgc
    43741 tgaagcgggg attaatcccg tacgcattga tgagatcgca gagtttttga atcaatgggg
    43801 taatgagtaa ttatttaatt gcagacgatc ggtcagtatc gttgtccgga taaagcgttc
    43861 acgccgcatc cggctaacaa tgacacgcac tgcagcgtgc tttaatgggt ttctcacgat
    43921 cgtaccgctc tgctcctcgc taagttgttc tgtgagttat agttatagag aaatcacggg
    43981 tacgaatgtt gaacagatgg cgacgccatc tgttcaacga ccaaccacac ggccctcatt
    44041 acgacccctg aacacaaacg gtaatggaaa atgtcattaa atattagtaa gatagcctct
    44101 ttacataaaa tcccttaata ttatgcgcca ggtattatcg tctcttttgg tgattgctgg
    44161 acttgtgagt ggtcaggcaa tcgccgcgcc tgaatccccc ccgcatgctg atatccgcga
    44221 cagcggtttt gtctattgcg tcagcgggca agtcaacacc tttaacccat ccaaagcgag
    44281 cagtgggtta attgtcgata cccttgccgc ccagttttat gatcgactgc tggatgtcga
    44341 tccctatacc tatcgcctga tgccagaact tgccgaaagc tgggaagtac tcgacaacgg
    44401 cgcgacctat cgcttccacc tgcgtcgcga tgtcccgttt caaaaaaccg cctggtttac
    44461 tcccactcgc aaaatgaatg ccgacgatgt ggtgtttacc ttccagcgaa tttttgaccg
    44521 caacaacccg tggcataacg tcaacggcag caacttcccc tacttcgaca gcctgcaatt
    44581 tgccgataac gtgaaaagcg tccgtaaact ggataatcat accgttgagt tccgtctggc
    44641 tcagccggat gcttcttttt tgtggcacct cgcaacccat tatgcttcgg tcatgtcggc
    44701 agaatatgcc cggaagttag agaaagaaga tcgccaggag caactcgacc gtcaaccggt
    44761 cggcactgga ccgtatcagt tgtcggaata ccgcgccggg caatttattc gcctacaacg
    44821 tcatgatgac ttctggcgcg gtaaaccgtt aatgccgcag gtggtggtgg atttaggctc
    44881 cggcggcacc ggacgtctgt cgaaactcct gaccggggaa tgcgacgttc tggcctggcc
    44941 tgctgccagc cagctatcca ttttgcgtga cgacccgcgc ttgcgtttaa cgctgcgtcc
    45001 tgggatgaac gtcgcctatc tggcatttaa caccgccaaa ccgccgctaa ataatcccgc
    45061 tgtccgccat gcgctggcac tggcgattaa taaccagcgc ctgatgcaat ccatctatta
    45121 tggtacggct gaaacggcgg cctctatttt accgcgcgcc tcgtgggcct atgacaacga
    45181 ggctaaaatt actgaataca atccggcgaa atcgcgcgaa cagttgaagt tgttggggct
    45241 ggaaaattta acgctgaaac tgtgggtgcc cacacgttcg caggcgtgga accccagtcc
    45301 actgaaaact gccgaactga ttcaggcgga tatggcgcag gttggcgtaa aagtggtgat
    45361 tgtgccggta gaaggtcgct ttcaggaggc gcggttgatg gatatgagcc atgatctgac
    45421 gttatccggt tgggcgacgg acagtaacga cccggacagt ttcttccgtc cgttactgag
    45481 ctgcgcggca attcattcac agaccaacct cacccactgg tgcgatccga aattcgacag
    45541 cgtgttgcgt aagacgctct cctcgcagca gctggcggcg cgtattgaag cctatgacga
    45601 agcgcagagt attctggcgc aggaattgcc cattttgccg ctggcgtcgt cattgcgttt
    45661 gcaggcctat cggtacgata tcaaaggtct ggtacttagc ccgtttggta acgcctcctt
    45721 tgctggggtg tatcgcgaga aacaggatga ggtgaaaaaa ccatgattat cttcacctta
    45781 cgccgcattt tgttattgat tgtcaccttg ttcctgctga ccttcgttgg cttcagcttg
    45841 agctatttca ctccacacgc gccgttacaa ggcgcgtcac tgtggaatgc ctgggtgttc
    45901 tggtttaacg gcctgatcca ctgggatttt ggtgtttcca gcattaacgg tcaaccaatt
    45961 gccgaacagt taaaagaggt cttcccggcg acgatggaac tgtgcatcct cgccttcggt
    46021 tttgcgctga ttgttggcat ccccgtgggg atgattgccg ggattacgcg ccataagtgg
    46081 caggataact tgatcaacgc catcgcctta ctgggctttt caatcccggt gttctggctg
    46141 gcgcttctgt tgacactgtt ttgttcactc acgctaggtt ggttgcccgt ttcagggcgt
    46201 ttcgatctgc tctacgaagt gaaaccgatt accggttttg cgttgattga tgcctggctt
    46261 tcggactcac catggcggga tgaaatgatc atgagcgcaa tccgccatat gatattgccc
    46321 gtgatcaccc tgtcggttgc gccaacaact gaagtgatcc gcctgatgcg tatcagcact
    46381 atcgaagtgt atgaccagaa ctatgtaaaa gcggcggcga cccgcggttt gtcgcgcttt
    46441 actattttgc gtcgccacgt tctgcataac gcgttaccgc cggttattcc tcgcctgggc
    46501 ttacagtttt ccaccatgct gacgctggcg atgatcaccg aaatggtttt tagctggccg
    46561 ggtttagggc gctggttaat taacgccatc cgccagcagg attacgcagc catttccgcc
    46621 ggagtgatgg tgtgtggctc actggtcatt attgttaacg tgatttctga tattttgggt
    46681 gccatggcta accctctgaa acataaggaa tggtatgcct tacgatagcg tatacagcga
    46741 aaagcgcccg ccgggcacgc tgcgtaccgc ctggcgcaaa ttttatagtg acgcctctgc
    46801 gatggtcggc ctgtacggct gcgcggggct ggctgtactg tgtatgtttg gcggctggtt
    46861 tgcgccttac ggcatcgacc agcaatttct cggttaccaa ttattgccgc catcatggtc
    46921 gcgctatggc gaagtttctt tcttcctggg gactgacgac ctcggacgcg atgttttaag
    46981 ccgtttactg agcggagctg cgccaaccgt gggtggcgca tttgtggtga cacttgccgc
    47041 gacgatctgt ggcctggtac tgggcacctt cgccggggcg acgcacggcc tccgctcagc
    47101 ggtgcttaac catattctgg ataccttact ggcgatccct tccctgctgt tggcaattat
    47161 cgttgtagcg tttgccggac cgagtttgtc ccacgctatg tttgctgtct ggctggcgct
    47221 gctgccgcgt atggtacgtt cgatttacag catggtgcat gacgaactgg aaaaagagta
    47281 cgttatcgcc gcccgtctgg atggcgcatc aacgctgaat attctctggt ttgctgtgat
    47341 gcctaacatc accgccgggc tggtcaccga gatcacccgc gcactgtcga tggcaattct
    47401 cgatatcgcc gcgctgggct ttctcgatct tggcgcacaa ctcccctcgc ctgaatgggg
    47461 agcgatgctc ggtgatgcgc tggaactgat ttatgtcgcc ccgtggactg tcatgctgcc
    47521 aggtgcggca attatgatta gcgtgctgtt ggttaacctc cttggcgacg gtgtccgtcg
    47581 tgcaattatt gcgggggtgg aataatgcca ttactcgata ttcgtaacct gactattgaa
    47641 tttaaaaccg gtgatgagtg ggttaaagcc gttgaccggg taagtatgac cttaaccgaa
    47701 ggtgaaatcc gcggtcttgt tggtgaatcc ggttccggca aaagtttgat tgcgaaagcg
    47761 atttgtgggg tgaataaaga taactggcgt gttactgcag accgtatgcg ttttgatgat
    47821 atcgatttgc tgcgtctctc cgcacgcgaa cggcgcaaac tggttggcca caacgtgtcg
    47881 atgattttcc aggaaccgca gtcgtgtcta gatccttcag aacgtgtggg tcgccagttg
    47941 atgcaaaaca tcccagcctg gacctataaa ggccgttggt ggcagcgttt tggctggcgc
    48001 aaacgccgtg ccattgaact gctgcaccgc gtgggaatta aagatcataa agatgcgatg
    48061 cgcagttttc cctatgagtt gaccgaaggt gaatgtcaga aagtgatgat agccattgca
    48121 ctggcgaatc aaccgcgtct gctgattgct gacgaaccga caaactcaat ggagccaaca
    48181 acccaggcgc aaatctttcg cctgctgacg cgtctaaacc aaaacagcaa taccactatt
    48241 ttgcttatca gccatgactt acaaatgctt agccaatggg cggataaaat taacgtgctt
    48301 tactgcggtc aaacggtgga aaccgcgccg agtaaggagt tagtgacgat gccacatcat
    48361 ccttataccc aggcgctgat ccgcgcgata ccagacttcg gaagcgcgat gccgcataaa
    48421 agtcgcctca atacgctacc cggcgctatc ccactgcttg aacagttacc gattggttgt
    48481 cgtctggggc cacgttgccc gtatgcacaa cgagaatgca ttgtgacgcc acgtttgacg
    48541 ggggcgaaaa atcatctcta tgcctgtcat ttcccgctga acatggagaa agagtgagat
    48601 gatcgaaacg ctgcttgaag tgcgtaattt aagtaaaacg ttccgctacc ggaccggttg
    48661 gtttcgtcgt cagaccgtag aagcggtaaa acccttgagc tttacgctac gtgaaggcca
    48721 gacactggcg attattggcg agaatggttc gggtaaatcc acgctggcaa aaatgctggc
    48781 gggaatgata gaacctacca gcggcgagtt attgattgac gatcatccac tgcattttgg
    48841 cgattattcc ttccgcagtc agcgtattcg catgattttt caggatcctt cgacctcgtt
    48901 gaatccccgt caacgtatct cgcaaattct ggattttcca ctgcgcctga acaccaacct
    48961 ggaaccggaa cagcgtcgca aacagattat tgaaacgatg cgtatggttg ggctgctacc
    49021 ggatcacgtc agttactatc cgcatatgtt ggcccccggg caaaaacagc gtctgggtct
    49081 ggcgcgcgcg ctgatattgc gcccaaaagt cattattgcc gatgaagccc tcgcttcact
    49141 ggatatgtcg atgcgttcgc agttgattaa tctgatgctg gaattacagg aaaaacaggg
    49201 catttcgtat atttatgtta cccagcatat cggaatgatg aagcacatta gcgaccaggt
    49261 gctggtgatg catcagggcg aggttgtcga gcgaggcagc accgcggatg tgctggcatc
    49321 gccgctgcat gaactcacca aacggctgat tgccgggcac tttggcgaag cattgacggc
    49381 ggatgcgtgg cgtaaggacc gttagtgttc tgcacctcat cctgacaggg gaacccccct
    49441 caggatgagg tattcattac ttttgattgt cgattctggt gagagcaacc aggaatgcca
    49501 gtgtataaca caccgtaatt gccagactca tttttaacag tgtttctccg ccaaatcccg
    49561 acaatggcgc ggctataccg ccgaagacaa acatgctcat ccccatcaac gcagacgccg
    49621 ttccagattc ctgcgtccct actgcactca tagcctctgc cccgccaaca gtgccgatgc
    49681 cgctacaaaa tgcaatagtg aaaaataatg ccaccagtgc cggaatgggc aattgtgtcc
    49741 atgcgcataa gaccgtgagc aatgcacaca aaattgccgc tatcaggcca cctcgcaaca
    49801 atgtcatcgc attaatccgt cgcgccagac gcgaaaagat ccatgaagca atgataagtc
    49861 cgatgccgtt aaggccaaac accaggctaa attgcattgg actaaagcca aattccttct
    49921 gcaacacgaa cgacgaagag ccgatatacg caaacaagcc tgccagcata aaactttgaa
    49981 tcaggcaaaa gcgcataaaa cggcggttct gcaccacgct tcgtccggtt aatagcaatg
    50041 atgagcccct tttattttct ggcaaggtct cattaataaa taacacgcag cccagcaaca
    50101 atacggtgct aatttcagcc attacccaga ataaagtgcg ccagtcaaaa gtgctaacaa
    50161 tgtacccgcc cagcactggc gacaacaccg gtgccaggcc attcaccgtc attaacagcg
    50221 caaaaaactg ggtcaacgtt actccctgat atttgtcacg agcaatagaa cgagagagca
    50281 ccgaaccacc cgcccccgcg atcccttgaa taaaacgcca gaccaccagc cagtaaatat
    50341 tgttcgttgt cgcgcacaaa atggaagaaa caataaacaa tagcaacgac aagattaacg
    50401 gacgtttacg ccctattttg tcgctcagag ggccaaataa cagttgtccg acgccaagac
    50461 cgatcagtga tgcagtcagc gttaattgcg ttatggtcgt ggttgccgcc agttgctcag
    50521 acatctccgg caaagccggc aaataaagat cggtacacat cgggccgata ccggctaaaa
    50581 gaccaagaat caatgcccat gaaagagaga ctctagccat attattcctt acttgagtga
    50641 tgacatgccg ccgcccagcg actgccagag cgtgattcgg tttgtaaaat cagtcagttg
    50701 cagtgccagc agctcctgct gtgcgctcca caagctgcgc tgcgccgtca acacggtcag
    50761 gtaatcacca acacccgtct gatagcgacg caagccaaca tcgaccgttt gttgctcagc
    50821 tttaacatac tggcgctgtg catccagttg ctcttcaagc gtggtccgcc gcgccagtgc
    50881 gttagcgaca tctttaaatg ctctctgaac ggttttttca taggtggcaa tgagtccacg
    50941 cttttgtgct tctgcgtaac gaagctgcgc cagattactt ccgccagtga acagcggtaa
    51001 tgtgaccgat ggcgtaaatg accagatctg cattccgtgg ctgaacaacg acgacaatgc
    51061 gtcactgcca acaccagcgc tggcagtcag cgaaatcgtc gggaaaaagt tagcccgcgc
    51121 cgcgccaata tcggcattgg cgctcttcaa attatgttcc gcttcctgga tatccgggcg
    51181 acgcagcaga acgtcagatg acactcccgc aggaaccaga cttatcatct gttccggcag
    51241 gctttccagt gttcccggta gcaaattttc ctcaagcgtt gtacctgcca gtaagttcag
    51301 cgcattttta tcctgcatca cttgtgtctg atagctggca acgctggcgc gcgcttgctg
    51361 ataaacactc atcgcctcac tgacatctgt cgctgccgcc gtgccaacct gttgctggcg
    51421 ctggataatt ttcaatgagt tttctgcact ggtcatcgtt tcttttgcca gggccaggtt
    51481 gctgttatcg gccgccagtg tcagccaggc ggtgctgatt tcagcaatca gcgttaaacg
    51541 ggtgttctgg gctgtaaatt cactggcaag ccaggtttct cgcgccgccc gagataaact
    51601 ctgattacga ccaaacagat ctaacgtaaa actggacacc gtgccgtcag cctcggcagt
    51661 ggtttccgta ccattcgcca gcgaacgact gcgggttgaa cttaaggccg cattcaccgt
    51721 cgggaataac gacgcattag tttgcccata gagcgcccgt gctgagtcaa tatcagcaat
    51781 cgctttttgc acatcgcggt tactgttcag tgcaatagtc accacctgct gcaatcgggg
    51841 atcgtggatc acctgctgcc agtcatggct gattgccttt ccctggccct gtgctcccgg
    51901 caaggtcgcc gggattgggg attcaggcgt gctgtagtgg ggatcgagcg agacacatcc
    51961 cgcactcaac agcaccagaa aaataagaga acgacgcaac ataacttact cctgacggcg
    52021 cgatttaccg gcaaacaaac gcttcaccag tacaaaaaac agaggaacaa agaaaatagc
    52081 cagcaacgta gcggtcagcg taccgccaat aatgcccgta ccaatggcga tgcggctgtt
    52141 cgcccctgag ccggttgcta tcgccagcgg cattaccccc cgcaataaac gccagcgagg
    52201 tcatgatgat tgggcgtaaa cgagtctgcg ccgcgcgtaa tgccgcacga ctcaaggaat
    52261 aaccctctgc aaccgccgct tcagcaaatt ccacaatcag aatggcgttt ttcgacgaca
    52321 agccgatagt ggttaatagc gccacctgga agtaaacatc gttgtttaaa tcacgcatcc
    52381 aggccgccag cgccgcgcca agaagcccca gagggatcac cagaatgacc gagaacggga
    52441 ctgaccagct ttcgtacagt gctgcaaggc agaggaaaac caccagaata gaaactgcat
    52501 acagactcaa tgcctgaccg ctggccagtt tctcctgcaa tgacaaacca ctccaggccc
    52561 aggttgttcc cgcaggaaga ctgtttgcca gttcctccat ttttgtcatt gcatcgccgg
    52621 aactaaagcc agtcgcattt tccccctgaa tttcataggc tgcagaaccg ttgtagcgca
    52681 ccagtctttc cggtccatac agccagcggg tggtcgcaaa agcagagaat ggtgtcatgg
    52741 cgttatcact accgcgcaca aaccatttac ctaagtctga cggcgcggaa cgaaattcac
    52801 tgtcgccctg aatgtagact tttttcacac gaccacggtc aataaagtca ttcacataag
    52861 taccgcccca cgcactggac agggtgtcgg tgacatcatt caaactcaac cctaatgaca
    52921 ccgctttatt actatcaata tctacctgca attgcggcat ttgtggcaaa tcattggcac
    52981 gaacagaatg taactccgga ctttgattcg ctttttctat caactgatta cgcatttgca
    53041 gtagtgtttc gcgatcggtt ccaccgttag ctaacaattc aaacgtaaaa ccattgcttt
    53101 gccccagccc atcaacggct ggcggcgtca tcgcaaatac cgtggcatca cgaattgtgc
    53161 ccagctcttt ggttgcccgt agggcgatag cctgggcggt gttttctgcc cctttacgtt
    53221 gagaccagtt tttcaacgaa acaaacgcca tcccggtgtt ctgtccgctg ccgctaaaac
    53281 tgaaaccatc aacggtaaaa atgacatcgg tatttgcttt ctcgttaatc aggaaccagt
    53341 caacaatctg gcgattcact tctgctgtac gggcagcggt cgccccggcc ggcagcgtgt
    53401 actgcaccat gatttcgccc tggtcttcag tgggtaagaa actgcccggc agtttccaca
    53461 tcatcagggc cattccacca cccagtacga gataaagccc catcgttcga gcagcacggc
    53521 gcaggacata aattacgcct cgctgatatt tatcttcagt acggcggtaa aagcggtcaa
    53581 atgcgccgaa aaagcctttt ttatgtggcg gaacatgctg gaggacggaa ccgcacaggg
    53641 cgggagtcaa ggtcaatgcc accaccacgg aaagcagcat tgcggagata atggttatcg
    53701 aaaactgacg ataaattacc cccgtagatc ccccaaagaa ggccataggc aggaatacgg
    53761 ctgacaacac cagcgcaatg gcaaccagtg cgccagaaat ctcgcccatt gatttttccg
    53821 tggcttcacg cgcgggcaac cctttgtcac gcataatgcg ttcgacgttc tccactacca
    53881 caatggcatc gtccaccagc aagccgatcg ccagcaccat agcaaacaac gtcagggtat
    53941 taatcgaata gccaaacaac gcaagaacgc cgaatgtgcc cagcaaaacg acggggacag
    54001 ctaacgccgg aatcagtgtg gcacgcaggt tttgcaggaa taaatacatc acgcaaacca
    54061 ccaatacgat ggcttcaaac agcgtctgaa ttacgtcctc tacagagatt ttaataaatt
    54121 cagtgctgtc tttcgggtaa gcgatgtcgt atccctgcgg catgttccgc tggaattcgg
    54181 caatcttatc cttgaccagc gtcgccgtat tcagcgcatt cgcaccgggc gaaagcatta
    54241 cagcaacccc ggcagacggg tgaccgttaa gtttgccgat agcggtataa tcttcactgc
    54301 ccatctcaac gcgagccaca tcttttatac gaacaactgc gctgtctgac tggcttttca
    54361 cgataatatt tttgaactga tccaccgtct gcaagcgaga ctgcgcacga accgttgcgg
    54421 tcagttgctg agtattcggt gaaggcaatg cccctatttt cccggcagta acctgcacgt
    54481 tttgcgcttc aatagcactt tgcacgtctg aaggcattag cgagtaagac gccagtttgg
    54541 ccgggtcaag ccagatgcgc atagcgtatt ccgcgccaaa gacttgcaga ctccccacac
    54601 cttcaacacg cgccagcggg tcctgaacgt tactaaccaa ccagtcggcg atatccgaac
    54661 tggaggcttt gtcagtggtg tcatacacgg cagcaatcag caaaaagttg ctttgtgatt
    54721 tctccaccgt aacacccgtt tgctgcactt cgctgggtag gcgcgactcc gcctgctgaa
    54781 ttttattctg cacctgtgca gtatctggat cggtaccttg ttcaaaggtc acattaatac
    54841 tgaccgaacc atcagagctg ctggttgagc tgaaatagag taaattatca agcccagtga
    54901 gttgttgctc gatgacctga gtcacgctgt tctccagcgt ttcggcagaa gcaccagtat
    54961 aagtggctga aattttaatg gtcggtggcg caacgtcagg atattgcgcg acgggcaatg
    55021 tgcgaatagc cagaatcccc gccaacataa tcaaaatggc gataacccag gcaaagaccg
    55081 ggcggcgaac aaaaaagcgc gaaaacatca ggcgttgcct ccattagcct gaacttcaac
    55141 agccttgacc gtctgccctg aagtgacttt ggcagaacct tcaacaatca gtcggtcgcc
    55201 gctgtgcagg ccgtttagca ccagccattt atcaccatat gtttctcccg tttcgagcgt
    55261 tcgctgctct actttattgt ctttattcac caccagcgca gttgcattgc ctttagcatc
    55321 gcgcgtgacg ccctgttgcg gcgcgagaat agcgtcttcc atcacgcctt catcgacacg
    55381 agcgcgaaca aacattcccg gtaataattg ctgttgtgga ttggggaaaa tcgcccgtaa
    55441 tgtcaccgaa ccggtagact catctacagc gacttcggtg agttccagac gccctttttc
    55501 gctgtaggtt gtgccatctt ccagaataag tgagacgctc atggtgtcac tgttggtcgc
    55561 cagtgactgt ttacgcagac gtaataaatc gacactggag cgagtgaggt cgacatacat
    55621 tgtatccaga ccacgaatag tcgttaacgc tgtatcttgc gacgcggtca ccagtgcgcc
    55681 aggggttacc gacgaaatgc caatgcgccc tgaaatcggt gcggttaccg tggtccagtc
    55741 aagattaatg cgcgcagttt ccagtgcggc ttttttcgcc gctacactgg ctttatcttg
    55801 tgcacaggta gactgagcat catcagcatc ctgttgtgaa acaccgttct ctttcaccag
    55861 tcgggcataa cgctgcgctt tctggcaatc ggcttttacc agtgcctgtg cttgttgtaa
    55921 tgctgcccga gcttcattcc atgcagcctg ataactggcc gcatcaatct ggtagagcgg
    55981 ctgtccagcc ttgaccagat caccttcctt aaataagcgt ttctggataa ttcccccaac
    56041 ctgcggacgt acttcggcac tgagcgcagc actggtgcgt ccggttaatt cactgactac
    56101 cgagaccggt tgagatttga ctgtaacaac gccgacttct gtttctgacg gggatgaatt
    56161 gttactttgc gtattatcac aaccagataa gagcagcatt gccactacag atgttgctat
    56221 atacttcatt attttctcca gggtgagaaa taccctcctc attaaaatta atgaggatat
    56281 gtcagggata ttcagttata acgaacccgc atcgcgaaat gaatttcgca atgaaataag
    56341 ggggattaat atcccccgct attggttaat tctgttctta tttatttatc aacatactaa
    56401 caatatcctg atatatttcc tgcagatgtt gtggatcgct tttttgcggt gttaaacgac
    56461 gataaaccgt accttccatc atggtagcaa acacttcaac gcagcaacga atgtgctcct
    56521 cgctaagatg gggatgcatt cttttcatat gtgcacaacc gttagcgaac attctggcgt
    56581 cagcttcttc aagcattgcc ataacccgcg ggttgcgtgt ggcttctgca gccacttcca
    56641 gcatcagcgc gtcgtcgtcc tcatttaagg atttacgcaa ggcaaggact tccgggaagt
    56701 gatcggtacg cgcgtcaatg tccatttggg caatacgaaa gtcgatgata cggcgaacca
    56761 tctcttcaat aatggcgtct ttgttggcga agtaacgata tatctgaccc acgctaagct
    56821 gagcttcgct ggcaatttgc gacatactgg cggcatgaaa cccgcagcga cgaaaacacc
    56881 gacgggcggc gttaatgatt tcatcctgac gctgtttgtg gcgtatttcc agcttgctgg
    56941 tcatttctgt ctcctggcct atcacttggt gagaatgatc gttctcattt ttgctgatta
    57001 tacgtttctc caaagtaatg gaaagtattt ttatcttatt catggtgcat attctgataa
    57061 tgaagagatt gtggagattc tgtgaagata gttgacaata ttaagttggg gtcaattcat
    57121 cagaaaggct gctacttgtt gtcgccagtt ctgttttcat catcaaactt atcggagcta
    57181 atacgaatag tttcatagtc agattctata agatatttcc ccaacctacc ccttgcaggc
    57241 gtcaagagta gataacgcaa aatgctctgt accgcagctt ctctccggta cagaaagcgc
    57301 aactatagcc acccacagcc aggttgatta taataaccgt ttatctgttc gtactgttta
    57361 ctaaaacgac gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc
    57421 tatgggtttt ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat
    57481 cgcctacggt atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca
    57541 gaacgacaaa ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt
    57601 tctgcaatgc gatgttgccg aagatgccag catcgacacc atgttcgctg aactggggaa
    57661 agtttggccg aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct
    57721 ggatggtgac tatgttaacg ccgttactcg tgaaggcttc aaaattgccc acgacatcag
    57781 ctcctacagc ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc
    57841 cctgctgacc ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg
    57901 tctggcaaaa gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga
    57961 aggtgtgcgt gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat
    58021 caaagacttc cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtaccgt
    58081 tactattgaa gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat
    58141 ctccggtgaa gtggtccacg ttgacggcgg tttcagcatt gctgcaatga acgaactcga
    58201 actgaaataa tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt
    58261 atacctttct gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag
    58321 gataacgatt tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa
    58381 agccactggt atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc
    58441 ccggaagccg caaaggtcag cgatcccttt ctactcgacg tgatccttga aaaagaaacg
    58501 ctggccccct tcctttcatg gctggaccct gcgcgtgttc ttgcagtgga gttgttccct
    58561 gaccagctta ccgtgacccg ttcacagacc ttcaccgctt atgaacgctt gtcgacggcc
    58621 ctgacggttg ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt
    58681 acgccgctcc ccgggcctga ttccaccagg gaaagtaatc atcggttggc acaaatcacg
    58741 caatatgccc gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat
    58801 ctgaatgacg tcggtcttac tgcctgggac tgtgtgatca ttaaccaaat cattggtttt
    58861 attggctttc aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc
    58921 tggttacccg ggctggagat acaaaactac gccgacgcgt cactgtttgc tgatgaatca
    58981 ttacgctggc gaagcagcta tgaggtggaa aaactacctg aagaacacac aaaaagttca
    59041 actgcagaac tttgccaact ggccgaaata ctctctctcc accctatttc actttccctt
    59101 ctcgaaaggt tgttaaacag cacacgggtt aatacacagc cggataatca gcttgcggcg
    59161 ttgttatgcg cgcggataaa tggcagtcct gcttgttttg ccgcctgtat ggattcatca
    59221 aatgaatata aaaaaatcag cccccttctg cgcaagggcg aaaatgaaat taaccaatgg
    59281 gctgaccgtc attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca
    59341 cccgatcgct ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg
    59401 cagattatta atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc
    59461 gcgttgggtg agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc
    59521 gctctaatgg ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct
    59581 taaacagcaa ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa
    59641 aggttttggc ttcctggaag tcgacgcgca aaaaagttat ttcattccgc cgccgcagat
    59701 gaaaaaagtc atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga
    59761 atccgcagag ccagaagaac tggttgaacc gtttctgact cgtttcgtgg gtaaagttca
    59821 gggcaaaaat gaccgtctgg ccatcgttcc tgatcatcca ctcttaaaag atgccattcc
    59881 ttgccgcgca gcccgtggtc tgaaccacga gtttaaagaa ggcgactggg cagttgccga
    59941 aatgcgccgt catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat
    60001 cacttttggt gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga
    60061 aaaagaagca ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtaaaga
    60121 tctgaccgcg ctggattttg tcaccatcga cagtgccagc acagaagata tggatgacgc
    60181 ccttttcgct aaggcgttgc cggatgacaa acttcagctg attgtggcga ttgccgatcc
    60241 aaccgcgtgg attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac
    60301 caactatctg cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg
    60361 ctcactgcgc gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga
    60421 tggcaccatt gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct
    60481 ggtgtatgac caggtttctg actggctgga gaataccggt gactggcagc ctgaaagtga
    60541 agcaattgcc gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg
    60601 tcataaccac gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa
    60661 aggtgaagtg ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga
    60721 agcgatgatt gccgctaaca tttgtgcagc tcgcgtactg cgcgataagc tcggttttgg
    60781 tatctataac gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct
    60841 gaaaacgcac ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa
    60901 actgcgtcgt gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt
    60961 ccagtcattt gctgaaatta gcactgaacc cggtcctcac tttggcctcg gtctggaagc
    61021 atacgccacc tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct
    61081 gaaagcggtt atcaaaggcg aaactgcgac gcgtccacag gatgaaatca ctgtccaaat
    61141 ggccgagcgt cgccgtctca atcggatggc agaacgtgat gttggtgact ggttatacgc
    61201 acgcttcctg aaagacaaag ccgggaccga cacccgtttc gcagcggaaa ttgtcgatat
    61261 cagccgtggc ggcatgcgtg ttcgtttggt tgataacggc gctatcgcct ttattcctgc
    61321 acctttctta cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcaccgtaca
    61381 aattaaaggt gaaacggttt ataaagtaac tgacgttatt gacgtcacca ttgccgaagt
    61441 ccgcatggaa acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca
    61501 ttccttatga atgggccgtt tatttccccg ctctaccttc atcatatccc ggcagttttt
    61561 taactgactt tcgtttgaaa actggcgttt ttccaaaact agattacaaa taaatatata
    61621 aaaaaaacaa tgtgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt
    61681 aaccgcggac tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac
    61741 aacgttgtac aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga
    61801 tgttttgcgc ttttctacca ccgaaaccac agaacctgaa cgtacgttgc agttatctgc
    61861 cgaacaggct gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct
    61921 gaccgtcgat gagagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga
    61981 atgggctggc aacgcgtctg catggcatga cacaccagca gttgctcgtg atttatcaca
    62041 cggactttcc tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga
    62101 cagccggggg aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaacga
    62161 acacgacgtc attggccaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc
    62221 atccaggcgc aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg
    62281 gataccaaca cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg
    62341 cagtggcaaa aacgagattt ttttaatctg ttccggcacc gacattaccg aagagcgccg
    62401 cgctcaggaa cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg
    62461 taacgcaatg caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg
    62521 ggtcgtgtat cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttatt
    62581 tggtgaccag ttattacgcg acgtgtcatt ggctatttta agttgtctcg aacatgacca
    62641 ggtgttggcg cgtccaggtg gcgatgagtt tctggtactg gcatccaaca cctcacaaag
    62701 cgcgctggaa gcaatggcat cacgaatttt gacccgctta cggctcccct ttcgcattgg
    62761 tttaattgaa gtttatacca gctgttcagt aggtattgca ctctctcccg aacatggttc
    62821 ggacagcgcg gctattattc gtcacgccga cacagcaatg tacacagcaa aggaaggcgg
    62881 acgaggacaa ttttgcgttt ttaccccaga aatgaatcaa cgggtatttg aatatctctg
    62941 gctggatacc aacttgcgta aagcactgga aaacgatcag ttggttattc actatcaacc
    63001 caaaatcacc tggcgtggcg aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc
    63061 tgaacgtggg ttgattccac cgttggactt catttcctac gcagaagagt cagggctaat
    63121 tgtgccttta ggccgttggg tgattctcga tgtcgtacgc caggtggcga agtggcggga
    63181 taaaggcatt aacctgcgag tggcggtaaa catttctgca cgtcagctcg ccgatcaaac
    63241 cattttcacc gccctgaaac aggttctcca ggaactcaat tttgaatact gccctattga
    63301 tgttgaattg acagagagtt gtctgattga gaatgatgaa ctggcactgt ctgttattca
    63361 acaatttagc caactaggtg cgcaagtgca tctggatgat tttggtaccg cctactcttc
    63421 actttcgcaa ctggcgcgct ttccgatcga tgccatcaaa cttgaccagg tttttgttcg
    63481 agatattcac aaacaacctg tctcgcagtc actggttcgg gcaatcgtcg ctgtggccca
    63541 ggcattgaat cttcaggtga tcgccgaagg tgtagagagt gcaaaggaag atgctttttt
    63601 aaccaagaat gggatcaatg agcggcaagg atttttgttt gccaaaccga tgcccgccgt
    63661 cgccttcgaa cgctggtata aacgctatct gaagcgcgca taagtagatg aatatatata
    63721 cttagcgcta tattccgtca ttatatatcg tattcccgga tgataattag ccgttgctgt
    63781 taaacacttc tcttttagcg gcaatttttg cattattcat tttccccctt ttgctataag
    63841 gatgtccttg atgtctgaat ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt
    63901 agatatcctt gtggcagacg attaccactc tgcgatccat aatctcgaga tacttaaagc
    63961 agaactcttg cgccaggttg ctgaatctac gccagacatc ccgaaggcac cgtgggagat
    64021 ttaacaatcg tgccttcagg ttttcctatt tgaggatgcc cggcgcgtca gtattttttt
    64081 atttagtatt ataacgttat aagaattaca gcgatgaatt cccgacaaca aactattcta
    64141 cagatggtca ttgaccaggg tcaggttagc gtaaccgatc tggcaaaagc cactggagtt
    64201 tctgaagtta ccattcgcca ggatctcaac accctcgaaa aactgagtta cctccgccgt
    64261 gcacatggct ttgcagtttc gcttgatagt gatgacgtcg aaacccgtat gatgagcaac
    64321 tatacgctga agcgtgaact cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc
    64381 atctttatcg aaaatggcag cagcaatgcc ctgctcgccc ggactctggg ggagcagaag
    64441 aaaaatgtca ctatcatcac ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct
    64501 tgtgaagtta ttttgctcgg tggcgtgtac cagaaaaaaa gcgaaagtat ggttggcccg
    64561 ttgacacgcc agtgcatcca acaggtacat ttcagcaaag catttattgg tattgatggc
    64621 tggcaacctg aaactggatt caccggtcgc gatatgatgc gtaccgatgt ggtgaatgcc
    64681 gtgctggaaa aagagtgcga agcgatagtc ctgactgaca gctcgaaatt tggtgctgta
    64741 cattcatact ccatcggtcc cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata
    64801 cgcgccagcg atctgatgca tcttgagcac agtaaactca ccgttcacgt cgttgacatt
    64861 taaccatttc ccttctctac ggatgatttg cagtttgaca aatcatccgc tctaagatga
    64921 ttcctggttg ataattaaga ctatttacct gttattaaca ctctcaagat ataaaattat
    64981 tatcagcgat ataacaggaa gtcattatca cctgcgtgat ataaccctgc gcgcgagcgg
    65041 atttcacgga ataatttcac caggcttatt cttagctatt atagttatag agagcttact
    65101 tccgtgaatc ataaattcag gagagagtat tatgtttgta acgagcaaaa aaatgaccgc
    65161 ggctgttctg gcaattaccc tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg
    65221 ggaccgcaac accgcaatcg gcgcgggtgc aggggcatta ggcggtgcag tactgaccga
    65281 tggcagtacg ttgggtacat taggtggcgc agctgtcggt ggtgttattg gtcaccaggt
    65341 cggtaaataa tcgtaaataa atgtcgatta tcgactcgta attataatac gcttgattct
    65401 gttcagtacg aaataactga ataatacaca cagtaaaata ttcgaaagcc acggatatat
    65461 ccgtggcttt ttatgttaac cgcctgcgag ttttaccttc atccctttcg cttcaagtag
    65521 tgattttaat aaatcacgct tatcgccctg gatttcaata actccatctt taactgctcc
    65581 gccgcagccg cattttttct tcagttccgc tgcaagtttt gtcagttcgg catcatcgag
    65641 atcgataccg gtaatcaggc aaacgccctt acctttacgt ccactggtct gacgctgaat
    65701 acgcaccaca ccatcgcctt tagagcgtac tggggccgct ttgggctcat caatacgccc
    65761 ggtttctgtt gagtagacca gacggctgtt ggaatcactc atcatgcact ccgctgtaaa
    65821 gaggcgttga tcgctttcag cgtctgcgct ggatctaccg attgcgttac cgggcgacca
    65881 atcaccatat aatcaacacc agccgccaac gcctgttctg gcgtcataat gcggcgctgg
    65941 tcaccagctt cactcccctg cggacgaatg cccggcgtaa ccagtttgaa ctcctgaccg
    66001 aatacctgtt taaagcgcac agcttcctga gcagaacaca ccacaccatc aaggccacat
    66061 ttttgcgtca gtgctgccag acgttctgca taatctgcag gtgacagtgt cacgccaaga
    66121 tcggccaggt cgctggcttc catgctggtc aacactgtca cagcaatcaa aagcggtgca
    66181 tctttgccaa acggaaccag tgcctcacgc gctgcggtca tcatacgcgc cccaccagag
    66241 gcatgaacat tcaccatcca cacgcctaag tcagctgcag cagcgacagc gtgcgctgca
    66301 gtgttgggga tatcgtggaa tttcaggtca agaaagatat caaaaccacg ctgttgaagt
    66361 tcgcgcacaa actgtggccc aaacaatgta aacatctctt tgccgacctt cagacgacaa
    66421 tcgcgtgggt cgatcttgtc gacaaaggcc agcgcggcat cacgattatg ataatcaagg
    66481 gcaacaacca caggagaatt cgtaacagcg cgggaagaag atgaagcagt taacgtcatg
    66541 accagacctt cttgatgatg ggcaccggaa gtggcgcgaa acaggtaaac ggcgagcatt
    66601 ctacccgtgg aaataaaaaa ttaacagttg cgatttctcc cctggcaggc tttttctgcg
    66661 ccgccgttgc cggagcacaa tgggacataa ataactagta tgttgtaact aaagtcggtt
    66721 tttttaaaaa attacaggcc atcaagaccg cgaatcggtt taatagttga ccaggcacga
    66781 caagacggac aatgccagta gagggtgtat gcggtaaaac cacatttctg gcagcgataa
    66841 cgaggcttac tacgtacctt ctcgccaacc atgtcacgca gcaccatcag gctctctttg
    66901 gcacgccctt cttccgcttc atttaagtgg taatccatta acttatggaa cacacgcatg
    66961 gtcggatgac gctgaagctg gcgcgtaata tagacctgtg cggcctcact accgtcgcgc
    67021 gcttcgatga tatcagcaag catcaattca gcatcggcac cggtgttctc ttccaccgcg
    67081 cgctgcagga attctgccca ttcggcagtt ttacccaact gctgatagca ggtttgcagc
    67141 atttccagcg tttcgctgac cagttctctg tcctgggata tcacgcgttg caaactttcg
    67201 acggctttgg cgtattctcc tttcgccata aacacgcgtc ccatcatgat ggatacgcgg
    67261 gcgctgtttt tatctgccgc cgcccctttt tttagcaagg tcatggcacg atcgagatcg
    67321 tcgctggcca tatgctgcag ggctaactca cagtagaaat gggcaatttc gacgcgctgt
    67381 ttatctttac ccagcttcac caggcgttcg gcaacatcaa ttgctttctg ccactcactg
    67441 gtagcctggt agatttgtag caactgttgc agcgcgccaa tgcggaagtc agtttcatcg
    67501 gtcagctgat tgaacatgtc ttccgcgcgg tcatataacc cggcggccat gtaatcacgc
    67561 cccagttgtt gaatcgccaa cagacgctgt tcataggtca gcgaggcgct ttccattagg
    67621 gtctgatgga tgcgaatagc gcgatcaact tcgccacgcg aacggaacag gtttccgagc
    67681 gtaaggtggg cttcaacggt gcctgtatcc tctttaagca tatcgagaaa caggtctacc
    67741 gctttatcct gttgattact aagcaggaag ttaacccccg ctacgtaatc acgcgacaag
    67801 cggttggctt catcttgctt gttttgttgc gcacttctgc ggcccatata ccagccatag
    67861 gcagcggcta caggcaaaag cagaaacaac aactccagca tagaaagtta ttccttcgcc
    67921 gctgacgagt gctgcactac agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc
    67981 tttattttac gttcagcgcg caccagggaa acacgaactc gcagccagaa caggccacaa
    68041 atcaaccaac cgatagcaaa acccgcagca aacaataccg ccagcaatgt ggaaatacgg
    68101 tactcccctt gcgctaacag ataattaaac gtcacctgtt gatcgttctg cgcacccaac
    68161 gtgaccgaaa tcacgaagat cgctaacacc agtaagaaaa tgagtaaata tttcacatta
    68221 cttcccgtta tgtggttaga gcgaataaat tatggtcaac ttaccgcaat agccctttaa
    68281 agtaccattt tcgcgccgtc tgcgaaatgg catccacgcg cattgttgat gatttaaggg
    68341 ctaaattcgg aaaatcaacc agcgttaact ttcttgttct cgttgcgcta tttcgcgatt
    68401 ttcttccgca ggtggtgtta atggcccgca aattcgttgc gcaagccacg ttgccaccgc
    68461 caccagtgcc cacgaaatca acgtagctac caccagatcg cgtggccaat gcatcccgag
    68521 cagcaggcgg cttcccatga ctcccgttgc ccagaccagc aagatagcaa tggttaacgt
    68581 tcgccgacgc ggccacagca aaccaacggc cagcagtgcc caactggcag caaacatcgt
    68641 gtgaccggaa ggaaaggcaa accccgtctc tttctgccag tgtgaacgca aatattgtgg
    68701 aatatttttc tcttcagcca actgttcttt cactagattt ccgcgttctg ctcgctttaa
    68761 agtgtagaac tcatcaaccg gaatatgatg tgttttttcc agccagataa caaaaggtcg
    68821 tggttcctgg actttgtctt tgatccagga tttaacgcct tgtcccacaa ggattgcggc
    68881 cgccagaatg gcaaataata caaaggcagc cttaatgcga aaacgcagac accagagaaa
    68941 ccagccgaat aaaatcaaat gtgtaatgac gccccagggc tgggtgacag tttcagtaac
    69001 ccaaaaagcc gcttttagta gccaactttg ttctccaggt tgccaacgcc agccagaaat
    69061 ccatacggct actggcataa caagcaatag tgcagctccc actgcggtac gtcttgcaat
    69121 cgaacgcatg gcctctcctt tttgataagt cccacaatca taactgaaaa tgccagttcc
    69181 aggaaaaatt gacagatttg tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa
    69241 ccccttctcg ttatggcaaa ataagccaat acagaaccag cattatctgg agaatttcat
    69301 gcagcttaaa cgtgtggcag aagccaaact gccaacccca tggggcgatt tcctgatggt
    69361 gggatttgaa gaactggcaa ccggacacga tcatgtcgcg ctagtctatg gcgatatttc
    69421 cgggcatacc ccggtacttg cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt
    69481 cagcttgcgc tgcgattgtg gcttccagct cgaagcggca ttgacgcaaa ttgccgagga
    69541 aggccgtggt attttgctgt atcaccgtca ggaaggtcgt aacattggtc tgctgaataa
    69601 aatccgcgct tacgcactgc aggatcaagg ttacgatact gtagaggcta accaccagtt
    69661 aggcttcgcc gctgatgagc gcgacttcac tctttgcgct gatatgttca aactccttgg
    69721 cgtcaatgaa gtccgcttgt taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc
    69781 ggggattaat attgttgaac gcgtaccatt gattgtaggt cgtaacccca ataacgaaca
    69841 ttatctcgat accaaagccg agaaaatggg ccatttgctg aacaaataac cctcttgcat
    69901 tgtgtaatta atttgcttgc cggaagcaaa ataaccggca agcaaatact tgttacttca
    69961 acatattacg aatgacataa tgcaaaatgc cgtcgttctg gtagtaagtc aactccgtcg
    70021 cggtgtcgat acgacaacgg cagggtacga cttcctggct accatccgcg cgcgtaagcg
    70081 tcaccggaac cgtcgcgccg ggttgtagat tttgcagatc gccaatatca atcttctctt
    70141 ccccggttag ccctaacgtt ttacgcgtta cgccttgcgg aaattccagc ggcaggatgc
    70201 ccatgccaat taaattcgaa cggtgaattc gttcaaacga ttcggcaatc accacacgaa
    70261 taccaagcag acgcggacct tttgccgccc agtcacggct ggagcctgat ccatactctt
    70321 tcccggcaat caccgccagc ggcgtttgct cctgcttata gcgcatcgca gcatcataaa
    70381 tagagactac gtcgctgtca ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca
    70441 tttcattacg gatgcgaata ttggcgaagg tgccgcgcat catcacttca tggttaccac
    70501 gccgcgaacc gtaggagtta aagtcttttc gctcaacacc ccgaccttgt aggtatcgac
    70561 ccgctgggct gtcgggctta atactgcccg ccggagagat atggtcagtg gtgactgaat
    70621 cccccagcat tgcgaggatc cgcgcaccgt gaatatcttc cacaggtgct ggcgttgcct
    70681 gcatttcatc aaagaaaggc gataaacgaa tataggttga atcctcctgc catccgtagg
    70741 tatcggatcg tgcgacgtta atttctttcc actctgcagt gccttcaaag acttctgcgt
    70801 actctttgcg gaacatttct gtggagactt gttctaccgc acgggcaatt tcttgtgccg
    70861 atggccagat atctttcaga tatattggat cgcctttacg atcatgacca ataggctcag
    70921 aagccaggtt gatattcata tttcccgcca gcgcataggc aaccaccagc ggcggcgagg
    70981 ccagccagtt agttttaacc agcggatgga tacggccttc aaagttacgg ttgccggaca
    71041 gcactgcacc gacggttaaa tcgccttttt tgattgccgt ttcgatagga tcgggcagcg
    71101 gcccagagtt accaatacag gtggtacaac cgtatcccac aaggttaaac cccagttcgt
    71161 cgagatacgg tgtcagtttc gcttttgcca gatagtcaga aacgactttc gaacccggtg
    71221 ccagcgacgc tttgacccac ggttgccgct tgaggccaag agttacggct tttttcgcca
    71281 gcaagcctgc ggccatcagc acacttgggt tagaagtgtt ggtgcacgag gttatcgcag
    71341 caatgaccac agcgccatca ggtaactgat actgatgtcc gttcataaca taatcgaccg
    71401 gctggcgatc tttatgcgtg gcattcactt ccagttcgtt actggcggca aatgcttttg
    71461 gtacatcggg cagtgcaacg cgatcctgtg ggcgtttagg ccctgccagg ctcgcttcaa
    71521 cgtcattcat atccagatct aacgtactgg taaaaatcgg ttcatcgccc gggttacgcc
    71581 acatgccctg cgctttggca tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc
    71641 ttaaacgcat gtaatcgagg gttacagcat cgattgggaa gaagccacag gtggcaccat
    71701 attctggcga catattggca atggtggcgc gatccgccaa cggtagtgaa tccagaccat
    71761 caccataaaa ttcgacgaat ttccccacca cgccatgttt gcgcagcatt tgggtaacag
    71821 tgagaaccag gtctgtggcg gtaatacctt cacgtaattt tccggtaagt ttaaagccca
    71881 ctacgtccgg gataagcatg gaaaccggct ggcctaacat tgcggcttct gcttcgatgc
    71941 cacccacgcc ccaccccagc acgccaaggc cgttgatcat agtggtgtgc gagtcagtac
    72001 caacgagtgt atccggataa gcaatccatt caccgtcctg caattcgctc cacactgctt
    72061 tgccgagata ttcgaggtta acctgatggc aaatgcctgt gcctggcggc acgacgctaa
    72121 accgactgaa cgcttgcttt ccccatttca ggaacacata acgttcgtgg ttgcgctcca
    72181 tttccaggcg tacgttttct tcaaatgcct catcatcacc aaaacgatcg acggtcaccg
    72241 agtggtcaat gaccaggtcg accggtgaga gcgggttaac ctttgcagta tcgccaccga
    72301 ggcgtttaac cgcttcgcgc attgccgcca gatcaacaac ggcaggtacg ccggtaaagt
    72361 cctgcatcag cacccttgcc gggcggtagg caatttcacg gtcagcatgg gcatttttca
    72421 gccatcccgc cagcgcgtgg atatcctctt cggtaaccga gttaccatcc tgccagcgca
    72481 gcagattttc gagcaaaact ttgagtgact tgggtagacg ggtgatatcg cccagtgatt
    72541 tggcagcaag cggcaggctg tagtagtggt aagttttatc tttggcctgc aacgcgtcct
    72601 tactggcttc tcgtagggtt gacgacatag ctcctcctta atgacagggt tgcgaagccc
    72661 tgattctctt cagggtgaat attaaagata acacaaacat cgcgtaacga tttgataaca
    72721 acccaaattg ataaaagagg agaaaccttg tggcaaagca aatgacaacc ccgccgcagc
    72781 ggggtcaagg atcagtgagt aatgatccag aggagttcgg cccagaagag catcgacaga
    72841 gaaaatgcac ccatccagga ccaatatttc atatttgtgt tgatcatagg ctacaccctt
    72901 aacggttatt actttgtgtt gagataactc aacctgttat tgctgcggtg tattaaaagc
    72961 cctttaattc attaaaaatg ggtaataata atgatgataa ttattgtttc ggctgatcat
    73021 cttcagcaaa cagttctata aaggcttgct gctgcgatgt tagttgccag tcgtctggaa
    73081 taacgacgga acattcccgt ttcgtttcgg gttcattgtc actatgctgg cataatggtg
    73141 acgactccag ttgctcctga ccaaccatag ttatccccac actttccaaa tcagcagtgc
    73201 gacaaccacc cagaaaagtg cagaaccaag aaaaaccgcc agccaggctt tacgttttaa
    73261 ttcaggatcg cggcggggtt cctgatttcc agaaggcatt gctaacctca tataatcgcc
    73321 atcacttatc agcaagacgt tgaacgattg gtgtaattaa tcatacgatg agacaaatcc
    73381 taaagaaact ttagtcaaaa atccagcgaa tttacgaatc gtctccagtg aataattcaa
    73441 tcttttgacc tgaaatagat atttgaaaac tggaatttgc gaggcgggta attagacact
    73501 actttacagg taatttaccc tataaatatg agggataaaa atattgtgca cggtatacaa
    73561 ataagtaaca aaagagaata tattccggca ccttcgctac ataaaaggtg ccgaaaataa
    73621 cgcaagaaat tatttttccg gcagttttat atctttaaac atgacctcaa tttcttcatt
    73681 ggagcgcaat gcgacagccg catcaacgac atcacgcgtt aaatgcggtg caaaacgctg
    73741 aatgaaatca tacatataac tgcgcaaaaa agtactacgg cgaaaaccaa ttttggttgt
    73801 actgtggctg aagatatcgt gagcatcaac acgcacaagg tccggatcgg cgaccggatc
    73861 caccgccatg ctggcaatga cccctacccc cagccctaac cggacgtaag ttttaatgac
    73921 gtcagcatcc gttgccgtga aaacgatacg cggcgttaac cctgcgcgat taaaggcagt
    73981 atccagttct gaacgtccag taaagccgaa ggtatatgtc accaacggat attgcgccag
    74041 ttcttcaatg gtaatggctt ttttgcctgc cagcgggtga tccggagtga ctacaatagc
    74101 ccgattccag tggtagcacg gtaacatcac taaatcttca tacagatgca gcgcttctgt
    74161 ggcgatagcg aaatctgcat tacctttaga gacggcatca gcaatttgtg tcggcgaacc
    74221 ctggtgcata tgcaaagaaa cgcggggata acgctcaata aagcctttga tgacgtttgg
    74281 taacgcgtag cgtgcctggg tatgcgtggt ggcgatatac agcgagcctt tatccggcca
    74341 ggtgtgctca ccggcaaccg attttatggc atcaactttc gacaggactt cgcgagcgat
    74401 acgaattatt tcttgtcctg ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa
    74461 aatttgaatg cctagctcgt cttccagcat tctgacttgt ttactgatcc cgggttgtga
    74521 tgtgtaaagt ccttccgctg ttgatgagac attcaggtta tgattgacca cctcaacaat
    74581 atagcgaagt tgttgtaatt tcatgttaaa ccatccactt agacgccatc agaacatcgt
    74641 tttttcgtca cacctggcgg gtgactaata cgatttaata ataaaaaagg tgctaactat
    74701 aaccactata tcatttatag ccttagtgta taggtgtgaa gaaaaaataa tatgcaataa
    74761 aaaagggccg ctttcgcgac cctttgttta taaaaacctg acagaattaa aggttatttt
    74821 ttcccttcaa cccatttgcc atcaacataa aatgctgacc agccagtcgc ctttccgtct
    74881 ttttccgaag agacatattg ctgtttggtt ttacggctaa agcgaaccat agtcttatta
    74941 ccttccggat cctgctgcgg cgcatcggcc agataacgca gtttttccgg cagacgatcg
    75001 cggaagcgat acagctcttc caccagcggc gcacgcgttt cacgcgattt cgggaaggta
    75061 ttggccgcca ggaacacacc ggcagcaccg tcacgcagca cgaaataggc atctgatttt
    75121 tcgcacggca gctccggtaa tggtaccgga tcttctttcg gtggagccac ttcgccgtta
    75181 cgtaaaatct tacgcgtgtt tttacactct tcgttggtgc aggccatata tttaccgaaa
    75241 cgccccattt tcaggtgcat ttcagaacca catttttcac actcaacgat cgggccgtca
    75301 taacctttaa tgcggaattc gccctcttcg atctcgtaac cgtcgcaggt tgggttatta
    75361 ccacagacat gcaacttacg tttcggatcg atgagatagc tgtccatcgc cgtgccgcat
    75421 ttggggcaac gacgttttgc gcgcagcgcg ttggtttcag cgtcttcgcc ttccagcacg
    75481 ttcagcactt cgttttccgg caccaggtta atggttgttt tgcaacgctc tttcggcggc
    75541 agcgcatagc cagaacagcc aaggaatacc ccggtgctcg ctgtgcgaat ccccattttg
    75601 cgaccacagg tcgggcagtc gatgctggtc agaaccatct ggttcggacg catacccccc
    75661 tcttccggat ctttttcagc tttatctaac tgctgagtga aatccgagaa gaagtgatcc
    75721 agtacagctt tccactctgc ttcgtgattt gccacctggt caaggctgtt ttccatctgc
    75781 gcggtgaagt cgtagttcat taactcgcgg aaattctctt ccaggcgatc ggtgacgatt
    75841 tcgcccattt tttccgcata gaaacgacga ttttctactc gcacatagcc acgatcctga
    75901 atggtcgaaa tgatcgacgc ataggtagac ggacgaccga taccacgttt ttccagttct
    75961 ttaaccagcg atgcttcact gaaacgggct ggcggcttgg taaagtgctg ggctggtgtc
    76021 agttcaacga gcgtcagagc atcgccttta tcgactgcag gtaagatacg atcttcatcg
    76081 cctttacgca gtgcaggcat cactttcgtc cagccatcaa agcgcaaaat acgaccgcgt
    76141 gctttcaggc ggaaatcgcc cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg
    76201 gtcatctggc aggcaacgaa ctgacgccag attaactggt acagtttctg cgcatctgct
    76261 tccatatcct tcagcgattc cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc
    76321 gcttcctgtg agttttcttt gctggcgtac tgattcggac tttccggcag atatttctta
    76381 ccaaaattat cgctgatata accgcgaacc atatttaccg cgtcctgact caggttagtg
    76441 gagtcggtac gcatgtaagt gatatagcct gcttcataca aacgctgcgc catcatcatg
    76501 gtttttttca cgccaaatcc aagacgggtg ctggcagctt gttgcagcgt agaggtaata
    76561 aaaggagcgc caggtttact ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc
    76621 gctttttcca gcagacttac cgcagcctga gtttgttctt tgttgaccgg acggaacggt
    76681 ttgtcgttct gatgagtcac ctgcaacgcc aacgcttcac cagatggcgt ggtcgtgctg
    76741 gcatcgactt cccagaactc ttccggcacg aacgctttaa tttcacgctc acgctcgacc
    76801 accaggcgga ctgccaccga ctgcacacga ccggcagaca gaccacgagc gatctttttc
    76861 catagcagcg gcgaaaccat ataccccacc acgcggtcca taaagcgacg cgcctgctgg
    76921 gcattaacac gatcaatatt cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt
    76981 ttagtaattt cgttaaacac cactcggcta tagcgcgcat catcaccacc aatcacttcc
    77041 cgcaggtgcc atgcaatggc ttccccttcg cggtcaaggt cggttgcgag atagatgtgg
    77101 tcggcttttt cagccagttg tttcagttca gagacgacct tctctttacc aggcaacact
    77161 tcatagtgcg cctcccaatt gtgccacggg tcaaccccca tacggttgac gagagcgcca
    77221 cgttcatcct ttttaggctt tttagccgtc ttggtggagg tagagtcggc actctttttg
    77281 gcagctgagc cactggtcgg caaatcgcgg atgtgaccga cgctggattt caccacgtag
    77341 tcactaccca gatacttgtt gatcgttttg gcttttgccg gggactcaac gatgacaaga
    77401 gctttaccca tattcacctt acctaatttg attcttccag gaatgcgtcg cacgttgata
    77461 aaccttccac tggcaacgag ttactgatat ggactcgctc tctgcggata tcaacccctt
    77521 gtcgtgactc tgttcacgaa aattcaggtg actgagttaa caggtcttgg cctacaggcg
    77581 ctatagcacg acgaaatttt ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag
    77641 cgtcacactg tacctgataa aatccgatac gcaactttat tagcatgcaa aatccaaagt
    77701 tgccagtaag gcgctcttgc caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt
    77761 agactaatgt ccttgtttaa ggagtaaata atgaacaaag agacacaacc catcgaccgc
    77821 gaaacgctgt tgaaagaggc caacaaaatc attcgtgagc atgaggatac gctcgcaggg
    77881 attgaagcca ccggcgtcac acagcgtaat ggcgtgctgg tatttaccgg cgactacttt
    77941 ttagatgaac aaggattgcc aacagccaaa agcaccgcgg tgtttaatat gttcaaacac
    78001 ctggcccatg tgctttcaga aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt
    78061 accctcgcgt ttgtctttta catcaatggc ttttgcccac gctgccacca gcgtagcaat
    78121 aatcgatcgg cgctctctgc cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc
    78181 atatagcgta cattgaccac ttctcggcct tccatcaggc taagaataac ttcatcactg
    78241 gtgttgattt catcaaccag gcccttctct accgcctgtt gtccgtacca gtgttcaccc
    78301 gttgccacct gttcaatatc cagagacgga cgcatacgct tcacaaaatc tttaaataac
    78361 tgatgcgttt cgttcagctc ttcgcggaat ttctcccgcc cttcttcggt attttcaccc
    78421 agcaaggtca gcgtacgctt atactgcccg gcggtgtgca gttcgatatc aatatctttg
    78481 cttttcagga agcggttaaa gttgggcatt tgcgccacca ccccaatgga acccacaata
    78541 gcaaacggtg cggaaacaat tttgtccgcc acacaggcca tcatgtaacc accgctggca
    78601 gcaactttgt ctaccgtaac agttaatgga atgtttttat cacgcagacg ctgcagctgc
    78661 gaagccgcca acccgtaacc atgcaccatg ccaccagggc tttccagacg gagcacaacc
    78721 tgatcctgcg gtttgaatgc tgcaagtacc gccgttatct cttcacgtag cgagttcact
    78781 tcatgggcgt ccatgctgcc tttaaagtcc agcacccaga cgcggggttt actgtcagtt
    78841 gacacctcgc ccagtttggc tttcgctttt gctgctttcg cttcttgctt gtgctttttc
    78901 ttctgcgctt tgtgccactg tttttgctga tgtgagtcca tcagcgccgc ggccagttct
    78961 tctttcatct ccttatactg ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt
    79021 ttattacgtt gagcaacatt gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg
    79081 gtaacgattt tcgccaaaaa caaaccatat tcagacaaca attccacgag tccccacctt
    79141 ggttaaacaa cgctttggct cacagtttac aacaggcatg ttaagccgtc tcgcagagat
    79201 gttattgcag cgaaagtatg acaaagtatc cattttgcgg tgttatcgcc cgaggctgat
    79261 tgaaatgccc ttctgtttca ggcataaagc cccaaagtca taaagtacac tggcagcgcg
    79321 acagacaacg tcgttgagga gtagccatgc attaccagcc aaaacaagat ttactcaatg
    79381 atcgcattat cctggtgacg ggagccagtg atggtattgg tcgtgaagcc gcgatgacgt
    79441 atgcacgcta tggtgcgaca gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg
    79501 tagccagcca cataaacgaa gaaactgggc gtcagccaca gtggtttatt ctcgatttgc
    79561 tgacctgcag gtccgaagat tgccaacaac tggcacagcg cattgccgtt aattatccgc
    79621 gtctggatgg tgttttgcat aatgccggat tactcggcga tgtttgtcca atgagcgaac
    79681 aaaatccaca ggtctggcag gacgtcatac aggtcaacgt taatgccacc tttatgctca
    79741 cccaggcact acttccttta ttactcaaat cggacgccgg ttcactggtc tttacttcat
    79801 caagcgttgg acgtcaggga cgagccaact ggggtgcata tgcagcgtcg aaatttgcca
    79861 ccgaagggat gatgcaggta ctggccgatg aatatcagca gcgcctgcgt gtcaactgca
    79921 ttaacccagg cggtacgcgc accgcaatgc gtgccagcgc cttcccgacc gaagatccac
    79981 agaaacttaa aacacccgct gatatcatgc cgctctacct ctggctgatg ggcgatgaca
    80041 gccgccgtaa aaccggcatg acctttgacg cccaaccggg ccgtaaacca ggaatttccc
    80101 aatgagtgat gaacgctacc aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg
    80161 tatggcccag gcccaggatg aacgcggtat tatcatcgtc tttaccggca atggaaaagg
    80221 caaaaccacc gcggcatttg gtacggcaac acgcgcagtt ggtcacggaa aaaaagtggg
    80281 cgtcgtgcag tttattaaag gcacctggcc taatggcgaa cgcaatctgc tggagccaca
    80341 tggcgttgag tttcaggtga tggcaacggg ctttacctgg gatacacaaa accgcgagtc
    80401 tgataccgcc gcctgccgcg aagtctggca acatgcaaag cggatgcttg ctgattcctc
    80461 actggatttg gttttgcttg atgagctgac ttatatggtg gcgtatgact atttgccact
    80521 ggaagaagtg ttgcaggcgt taaatgaacg tccacatcag cagacggtga ttatcacggg
    80581 tcggggctgt catcgggata tactcgaact ggcggatacg gtaagtgagt tacgccccgt
    80641 gaaacatgcg tttgatgctg gcgtaaaagc gcagataggg attgattact aatctgtaaa
    80701 aatgccggat atgacgtccg gcatacgttg attaaccgtt attacgccca ccagaacgac
    80761 gtccgccgct cacctgactg tgacgtttca ccgcacgacg aatctgattc gccttcatgc
    80821 gacgacggtc tttttctaca gcgactttag agctggtttc tggcggcaac tccaccagtt
    80881 cgcgcagata gttagtctgg gcaagatcca gctccgtcca gccgccgcgt ggcagacctt
    80941 tcggcagtgg gatatcaccg taacgaacac ggatcaggcg gcttacctgc acgccaaccg
    81001 cttcccacag acgacgaact tcacggttac ggccttcggt cagagtcacg ttgtaccact
    81061 ggttgatccc ttcgccgccg ctgaacttga tggttttaaa ggctgccgga ccatcttcca
    81121 gctgcacacc acggcttaaa tcacgcagtt tcgcgtcgtc aacctgacca aatacacgca
    81181 cggcatattc acgttcaact tcacggcttg gatgcattaa acggttagcc agttcaccgt
    81241 cggtggtgaa cagcaacaga ccacaggtgt taacgtccag acgacctacg gcaatccagc
    81301 gtgcaccgcg cagctttggc agacggtcaa acaccgttgg acgcccttcc ggatcgttac
    81361 gggtacacaa ctcaccttcc ggtttataat aggccagaac gcgacaaatt tgttcagcgg
    81421 actcacgtac cgaaatcagg tgaccatcga tacggatttt caatccaggg gtaacttcaa
    81481 cgcgatcgcc gagtttagca attttgccat caacactcac acgaccggct tcaataatag
    81541 attcgatttc acggcgagaa ccgtggccgg cacgcgccag cactttctgt agcttttcgc
    81601 tcatagagct tccttttggt gtcgccttcc caggcgtctg atatgctttt gtgcaacagt
    81661 gagtcagaac ttacatctga ctcactgata gtaaagtctg tttgaactct tcaggcctta
    81721 agtaaacgac tcgacgtttt aactggcctg gctttgaggc gcaacatact acactatctt
    81781 tatggttaaa gcttgtttcg attaacatca gcttaattac cagccaccac cgcctccgcc
    81841 acccgcagca ccaccggaat aacttcgccc caataaactg gtattggccc ccagactcag
    81901 gtgcatctgt aatgagaaca aaacgccact acgggcaatt tccggggcgc gaacagccgc
    81961 tgtgagtgat ggggtgatac gtgctaccca ttctcttcca aggtttgctg agacagccca
    82021 acctagcaac gcgtaatcca gtttccgtgt ttcccctttc cgccgacgcc cactgtgtgt
    82081 tgctgcctct ttacgcgcga gatagttaac gatagcttca ccgtgggcat aacgttgctg
    82141 cccagtttgg gtaaactttg gcagaaaaat ataccccatg ccagtgagat aaccggtcag
    82201 gaaaacagcg ctaaaataac ccgctggcat ataccagtaa aagaatacat ctccgacatt
    82261 gatatagaga gaataaaagg ccacgcctga acaaatcagc ggtaaaaaca aagtgatgat
    82321 tacacgatta acgcaaggct tgtcatcatc gcgggcaaac aaaagctcaa gggaacaaaa
    82381 taaaaaagga agaagccagc agatcataat aaacatatcg cccaccagac tcattcctgc
    82441 ccagcctaca ggactcaggt taccgtaaag aatgaccgcc aatattgcca gtccacccca
    82501 ttgcaaaaaa gtatcaggcc ggtaaaacga agacttttta cgcggcagat aaaatttctg
    82561 catccgtaaa acatctaccg acgtaaattg cggcctgcgc ttccagagat ataaccatcc
    82621 acaaaccagg aatagacacg gaaaccaaat taacaacgat gacgtggagg gtaaaagaag
    82681 atggctgaaa atattcgtcg tttgtggagc cggggcattg ctaagtaacg agtgaggcca
    82741 acggtagagt accgtgaaat cttcacgata aaacggatcg ctggattcaa ttcttccgtc
    82801 ctcaaggatg cggccatttc gatatgtgtc gccctcttct ccagtaaaga gatcgatttc
    82861 gctaaatgga ttacccgcga tatcagggaa cttgagtgaa aatcgggtct tatagatttc
    82921 aaacggccag tggttaccag tcacgttcca gattaacaga tctgaatctc cctcacgcag
    82981 gaaagcattt ttaacctgat aatgaatttg ataatgatag tcgccttgag gcaagaaacg
    83041 ttgtttatcg cccacgacaa tactcattaa cccgatatgc cagtcaagat gccagggttc
    83101 aggaatacca tcacgggtga cgccaacaat atgatagttc agcagtgcag cgtccccatc
    83161 aggctgcatc catagtcgtg gtaaatcacg ataaaatccg tgcttaatct gctgatttcg
    83221 cgcctgaaaa tgaatatttt ctttgatgtc gagcacccca tccggttgca gagtggcttc
    83281 aacatcaaag agcgagatcc cttcttcata agaaggcatc tccgtgtttt tcgcataact
    83341 taacgaagag aaaaaaagcc caactatcaa cagcaaaatg cagcgaaact tccctgccat
    83401 agtcttccat ccgtggaacg caatttgtta taagaaaggc ttcacatcac ctacgccttc
    83461 acgcaccacg accggcgtat catcggtaag atcaataacc gttgttggtt tctgcccgag
    83521 atagccgccg tgaataatca aatcgacctg tttttccaga cggtctttga tttcttcggg
    83581 atcggattcg gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc
    83641 cagtgcttca agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt
    83701 gcgtttttcc tgcaacagac ggcgtggcac ctccttcgtc cccttcagga tgaaggtata
    83761 gttgcccggc gtgttgtttt tcattaaacg aaacgcaacg ttatcgacaa atgaataggt
    83821 cgagagttca gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg
    83881 aatacgacaa atacgctcca tggcgttttt atcttcaatt ttacagccga gcgcatagcc
    83941 ggaatcagtt ggataaacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat
    84001 tagacgttgc tgcgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct
    84061 gtggtgttct gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag
    84121 agtttacgac ccagttcgat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc
    84181 cataaatggt gctggcgggc aagcgtcgcc agctgggtac gttcattggg cgattgctgg
    84241 cactgcgcga cttccatcgc gtcgccgtcg tgttcggcaa aatgcgcaac caatcttttc
    84301 agccatttag cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa
    84361 tgatgaatga catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt
    84421 ttcccgcgcg ccagatactt tttaaagaca tccgccattg aactggcttt gccgcactca
    84481 acaaggaaac gcgcaaaatg accgcgcgtc actgcgccgc cctgcgccag tcgttgtgcg
    84541 ccttccagcg ccccaggtat atgcgctttt tcaagtcttt cggcaatttg ctgcgcccgc
    84601 tgattgcgcc gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca
    84661 atattcagcc cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg
    84721 ggaataagat tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cgcgattgca
    84781 gctgtggtgt catggtcggt gatcgccagg gtgccgacgc gcatctcgac tgcacggtgc
    84841 accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa
    84901 atcactgcgt aattcgtgtc gctcaaggtg cactcccgtt ctggataatg ttttttgcgc
    84961 cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatc aatcatcgaa
    85021 ctagttaact agtacgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt
    85081 actgaaaggt tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa
    85141 tcagataccc agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa
    85201 tgcaaacaca aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaatc
    85261 ccaccgcgct ttttcaccaa ttgtgtgggg atcgtccggc aacgctgctg ctggaatccg
    85321 cagatatcga cagcaaagat gatttaaaaa gcctgctgct ggtagacagt gcgctgcgca
    85381 ttacagcttt aggtgacact gtcacaatcc agtccctttc cggcaacggc gaagccctgc
    85441 tgacactact ggataacgcc ctgcctgcgg gtgtggaaaa tgaacaatta ccaaactgcc
    85501 gtgtgctgcg cttcccccct gttggtccac tgctggatga agacgctcgc ttatgctccc
    85561 tttcggtttt tgacgctttc cgcttattgc agaatctgtt gaatgtaccg aaggaagaac
    85621 gagaagccat gttcttcggc ggcctgttct cttatgacct tgtggcgggg tttgaagatt
    85681 taccgcaact gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaactc
    85741 tgatggtgat tgaccatcag aaaaaaagca cccgcattca ggccagcctg tttgctccga
    85801 atgaagaaga aaaacaacgt ctcactgctc gcctgaacga tcttcgccag caactgaccg
    85861 aaaccgcgcc accgctgccg gtggtttccg tgccgcatat gcgttgtgaa tgtaaccaga
    85921 gcgatgaaga gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gccggagaaa
    85981 ttttccaggt ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct
    86041 attacgtgct gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt
    86101 tcaccctgtt tggcgcgtcg ccggaaagtt cgctcaagta tgacgccacc agccgccaga
    86161 ttgagatcta cccgattgcc ggaacacgtc cacgcggtcg tcgcgccgat ggctcgctcg
    86221 acagggacct tgaaagccgt attgaactgg aaatgcgtac cgatcataaa gagctttctg
    86281 aacatctgat gttggtggat ctcgcccgta atgacctggc acgcatttgc acccccggca
    86341 gccgctacgt cgccgacctc accaaagttg accgttactc ttacgtgatg cacctggtct
    86401 cccgcgtggt cggtgagctg cgccacgatc tcgacgccct gcacgcttac cgcgcctgta
    86461 tgaatatggg gacgttaagc ggtgcgccga aagtacgcgc tatgcagtta attgccgagg
    86521 ctgaaggtcg tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcacggcg
    86581 atctcgacac ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gctaccgtgc
    86641 aagccggtgc tggcgtagtc cttgattctg ttccgcagtc ggaagccgac gaaacccgta
    86701 ataaagcccg cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagacgttct
    86761 aatggctgac attctgctgc tcgataatat cgactctttt acttacaacc tggcagatca
    86821 gttgcgcagc aatggtcata acgtggtgat ttaccgcaac catattccgg cgcagacctt
    86881 aattgaacgc cttgcgacga tgagcaatcc ggtgctgatg ctttctcctg gacccggtgt
    86941 gccgagcgaa gctggttgta tgcctgaact gcttacccgc ctgcgcggta agctgcctat
    87001 tattggcatt tgccttggtc atcaggcgat tgtcgaagct tacgggggct atgtcggtca
    87061 ggcgggcgaa attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt
    87121 tgccggatta acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaatat
    87181 tccggccggt ttaaccatca acgcccattt taatggcatg gtgatggcgg tgcgtcacga
    87241 tgcagatcgc gtttgcggat tccagttcca cccggaatcc attctcacca cccagggcgc
    87301 tcgcctgctg gaacaaacgc tggcctgggc gcagcagaaa ctagagccaa ccaacacgct
    87361 gcaaccgatt ctggaaaaac tgtatcaggc gcagaccctt agccagcagg aaagtcatca
    87421 gctattttca gcggtggtac gtggtgaact gaaaccggaa cacctggcgg cggcgctggt
    87481 gagcatgaaa attcgcggcg agcacccgaa cgagatcgcc ggagcagcaa ccgcgctact
    87541 ggaaaacgcc gcgccgttcc cgcgtccgga ttatctgttt gccgatatcg tcggcaccgg
    87601 cggtgacggc agcaacagca tcaatatttc caccgccagt gcgtttgtcg ccgcggcctg
    87661 tgggctgaaa gtggcgaaac acggcaaccg tagcgtctcc agtaaatctg gctcgtcgga
    87721 tctgctggcg gcgttcggta ttaatcttga tatgaacgcc gataaatcgc gccaggcgct
    87781 ggatgagtta ggtgtatgtt tcctctttgc accgaaatat cacaccggat ttcgccatgc
    87841 aatgccggtt cgccagcaac taaaaacccg caccctgttc aatgtgctgg ggccattgat
    87901 taacccggcg catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc
    87961 gattgccgaa accttacgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg
    88021 gatggatgaa gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcgaaat
    88081 taagagctat caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact
    88141 ggcaggcgga acaccggaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg
    88201 cgacgccgcc catgaagcag ccgtcgcggc gaatgtcgcc atgttaatgc gcctgcatgg
    88261 ccatgaagat ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc
    88321 ttacgacaga gtcaccgcac tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa
    88381 aatcgtcgca gacaaggcga tttgggtaga agcccgcaaa cagcagcaac cgctggccag
    88441 ttttcagaat gaggttcagc cgagcacgcg acatttttat gatgcgctac agggtgcgcg
    88501 cacggcgttt attctggagt gcaagaaagc gtcgccgtca aaaggcgtga tctgtgatga
    88561 tttcgatcca gcacgcattg ccgccattta taaacattac gcttcggcaa tttcggtgct
    88621 gactgatgag aaatattttc aggggagctt tgatttcctc cccatcgtca gccaaatcgc
    88681 cccgcagccg attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg
    88741 ctattaccag gccgatgcct gcttattaat gctttcagta ctggatgacg aacaatatcg
    88801 ccagcttgcc gccgtcgccc acagtctgaa gatgggtgtg ctgaccgaag tcagtaatga
    88861 agaggaactg gagcgcgcca ttgcattggg ggcaaaggtc gttggcatca acaaccgcga
    88921 tctgcgtgat ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca
    88981 caacgtgacg gtaatcagcg aatcaggcat caatacttac gctcaggtgc gcgagttaag
    89041 ccacttcgct gacggttttc taattggttc ggcgttgatg gcccatgacg atttgcacgc
    89101 cgccgtgcgt cgggtgttgc tgggtgagaa taaagtatgt ggcctgacgc gtgggcaaga
    89161 tgctaaagca gcgtacgacg cgggcgcgat ttacggtggg ttgatttttg ttgcgacatc
    89221 accgcgttgc gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta
    89281 tgttggcgtg ttccgcaatc acgatattgc cgatgtgctg gacaaagcta aggtgttatc
    89341 actggtggca gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga
    89401 agctctgcca gcacacgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc
    89461 ccgcgagctt cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca
    89521 acgtttcgac tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg
    89581 cttaggcgca gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa
    89641 ttctgctgta gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca
    89701 gacgctgcgc gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt
    89761 gagtttggcg gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa
    89821 gcttttgtca gcgcgcaaaa agatcctgaa tttcaggctc agttcaacga cctgctgaaa
    89881 aactatgccg ggcgtccaac cgcgctgacc aaatgccaga acattacggc tggcacgaac
    89941 accacgctgt atctgaagcg cgaagatttg ctgcacggcg gcgcgcataa aactaaccag
    90001 gtgctcggtc aggctttact ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc
    90061 ggggccggtc agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc
    90121 cgtatttata tgggggccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgt
    90181 ttaatgggtg cggaagtgat cccggtgcat agcggttccg cgacgctgaa agatgcctgt
    90241 aacgaggcgc tgcgcgactg gtccggcagt tatgaaaccg cgcactatat gctgggcacc
    90301 gcagctggcc cacatcctta tccgaccatt gtccgtgagt ttcagcggat gattggcgaa
    90361 gaaaccaaag cacagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt
    90421 gttggcggcg gttcgaatgc catcggcatg tttgcagatt tcatcaacga aaccaacgtc
    90481 ggcctgattg gtgtggagcc tggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg
    90541 ctaaaacatg gtcgcgtggg tatctatttc ggtatgaaag cgccgatgat gcaaaccgaa
    90601 gacgggcaga ttgaagaatc ttactccatc tccgccggac tcgattttcc gtccgtcggc
    90661 ccacaacacg cgtatcttaa cagcactgga cgcgctgatt atgtgtcaat taccgatgat
    90721 gaagcactgg aagccttcaa aacgctgtgc ctgcacgaag ggattatccc ggcgctggaa
    90781 tcctcccacg ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga aaaagagcag
    90841 ctactggtgg ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt
    90901 ttgaaagcac gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg
    90961 agcgcaaaga aggcgcattc gttcctttcg tcacgctcgg tgatccgggc attgagcagt
    91021 cgctgaaaat tatcgatacg ctaattgaag ccggtgctga cacgctggag ttaggcatcc
    91081 ccttctccga cccactggca gatggcccga cgattcaaaa cgccacactg cgtgcctttg
    91141 cggcaggtgt gaccccggcg cagtgctttg agatgctggc actcattcgc cagaaacacc
    91201 cgaccattcc cattggcctg ctgatgtatg ccaacctggt gtttaacaaa ggcattgatg
    91261 agttttatgc ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccag
    91321 ttgaagagtc cgcgcccttc cgccaggccg cgttgcgtca taatgtcgca cctatcttca
    91381 tctgcccgcc aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt
    91441 acacatattt gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc
    91501 tcaatcatct ggttgcgaag ctgaaagagt acaacgcggc acctccattg cagggatttg
    91561 gtatttccgc cccggatcag gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt
    91621 ctggttctgc gattgttaaa atcatcgagc aacatattaa tgagccaaag aaaatgctgg
    91681 cggcactgaa agcttttgta caaccgatga aagcggcgac gcgcagttaa tcccacagcc
    91741 gccagttccg ctggcggcat tttaactttc tttaacgaag ccggaaaaat cctaaattca
    91801 tttaatattt atctttttac cgtttcgctt acccctgtcg aacgtcaact tacgtcattt
    91861 ttccgcccaa cagtaatata atcaaacaat ttaatcccgc aacataacac cagtaaaatc
    91921 aataattttc tctaagtcac ttattccgca ggcaattgtt aatatatcaa gaacgttcgt
    91981 cataatatat tttcctactt tcttctcgtt acgcttaatt tgactaatcc tcattagcga
    92041 ctaattttaa tgagtatcga cacacaacac tcatattaat gaaacaatgc aacgcaacgg
    92101 gagaaataac atggccgaac atcgtggtgg ttcaggaaat ttcgccgaag accgtgagaa
    92161 ggcatccgac gcaggccgta aaggcggtca gcatagcggc ggtaatttta aaaatgatcc
    92221 gcaacgcgca tctgaagcgg gtaaaaaagg cggtcaacaa agcggtggta ataaatcagg
    92281 aaaatcctga ttcaccgtta attattaata tcagctgata aagataattg catctgcggg
    92341 ccgtatggct cgcagtgatt tcactggaga aaaatatgaa tatgaagacc attgaagatg
    92401 tatttattca cctgctttca gatacctaca gcgcagaaaa acaattaacc cgggcactgg
    92461 caaaactcgc aagagcaaca tcaaacgaaa aattaagtca ggcttttcat gcgcacctcg
    92521 aggaaacaca tggacagatt gaacgtattg atcaagttgt ggaatcagaa tcgaatctga
    92581 aaattaagcg catgaaatgt gtggcaatgg aaggtcttat tgaagaagct aatgaggtca
    92641 tcgaaagtac cgagaaaaac gaagtgcgtg atgccgcact gattgccgca gcacagaaag
    92701 tcgagcatta tgagattgcc agttacggga cattagcgac gctggctgaa caattaggtt
    92761 accgtaaagc agcgaagctt ctgaaagaga ccctggaaga agaaaaggcc accgacatca
    92821 aactgactga tctggccctt aataacgtaa ataagaaagc cgaaaataaa gcctgaaata
    92881 tgaactttaa cttttggtca ttttataaag aggacatttt catgaatcgt attgaacatt
    92941 atcatgactg gttacgtgac gcccatgcaa tggaaaagca agccgaatct atgcttgaat
    93001 ccatggccag ccgtatagat aattatcctg aactacgtgc tcgtattgaa caacatctta
    93061 gtgaaaccaa aaaccagatt gttcaactgg aaactattct tgatcgtaat gacatttcac
    93121 gttcagtcat taaagattcc atgagtaaaa tggctgcgct tgggcagtca atcggtggta
    93181 tattcccttc tgatgaaata gtcaaaggct ctattagcgg atatgtcttc gagcaatttg
    93241 aaatcgcctg ttacacctct ctattagcag cagcaaaaaa tgccggtgat acagcctcaa
    93301 ttccgatcat cgaagcgatt ttaaatgagg aaaagcaaat ggccgactgg ctgattcagc
    93361 atattccgca aacaactgag aaatttttaa ttcgctctga aactgatggc gtagaagcga
    93421 agaaataata ccctttatgc catgtcctta ttgaccccgt atattacggg gtcgtttttg
    93481 tgcggaatta aaaacgatat cctgctgaga acataaacac ccacggatcg aggcgtacgc
    93541 tatcatgttg ctgtgcaccg cccagtttat aattggcggt ggtatcgata t
//
LOCUS       DAZKIF010000003        86150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZKIF010000003 DAZKIF010000000
VERSION     DAZKIF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.003"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            456..905
                     /gene="alaE"
                     /locus_tag="ACHOTW_000193"
     CDS             456..905
                     /gene="alaE"
                     /locus_tag="ACHOTW_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP9842379.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(942..1286)
                     /gene="ygaC"
                     /locus_tag="ACHOTW_000194"
     CDS             complement(942..1286)
                     /gene="ygaC"
                     /locus_tag="ACHOTW_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP9842380.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1438..1767
                     /gene="ygaM"
                     /locus_tag="ACHOTW_000195"
     CDS             1438..1767
                     /gene="ygaM"
                     /locus_tag="ACHOTW_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP9842381.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1917..3251)
                     /locus_tag="ACHOTW_000196"
     CDS             complement(1917..3251)
                     /locus_tag="ACHOTW_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9842382.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            3339..3770
                     /locus_tag="ACHOTW_000197"
     CDS             3339..3770
                     /locus_tag="ACHOTW_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9842383.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3979..4224
                     /gene="nrdH"
                     /locus_tag="ACHOTW_000198"
     CDS             3979..4224
                     /gene="nrdH"
                     /locus_tag="ACHOTW_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9842384.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            4221..4631
                     /gene="nrdI"
                     /locus_tag="ACHOTW_000199"
     CDS             4221..4631
                     /gene="nrdI"
                     /locus_tag="ACHOTW_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9842385.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4604..6748
                     /gene="nrdE"
                     /locus_tag="ACHOTW_000200"
     CDS             4604..6748
                     /gene="nrdE"
                     /locus_tag="ACHOTW_000200"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9842386.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6758..7717
                     /gene="nrdF"
                     /locus_tag="ACHOTW_000201"
     CDS             6758..7717
                     /gene="nrdF"
                     /locus_tag="ACHOTW_000201"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9842387.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
     gene            8073..9275
                     /gene="proV"
                     /locus_tag="ACHOTW_000202"
     CDS             8073..9275
                     /gene="proV"
                     /locus_tag="ACHOTW_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP9842388.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPNELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEIINNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            9268..10332
                     /gene="proW"
                     /locus_tag="ACHOTW_000203"
     CDS             9268..10332
                     /gene="proW"
                     /locus_tag="ACHOTW_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP9842389.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10389..11381
                     /gene="proX"
                     /locus_tag="ACHOTW_000204"
     CDS             10389..11381
                     /gene="proX"
                     /locus_tag="ACHOTW_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP9842390.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11573..12757
                     /locus_tag="ACHOTW_000205"
     CDS             11573..12757
                     /locus_tag="ACHOTW_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9842391.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12881..13618
                     /gene="ygaZ"
                     /locus_tag="ACHOTW_000206"
     CDS             12881..13618
                     /gene="ygaZ"
                     /locus_tag="ACHOTW_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP9842392.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13608..13943
                     /gene="ygaH"
                     /locus_tag="ACHOTW_000207"
     CDS             13608..13943
                     /gene="ygaH"
                     /locus_tag="ACHOTW_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP9842393.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            14034..14564
                     /gene="emrR"
                     /locus_tag="ACHOTW_000208"
     CDS             14034..14564
                     /gene="emrR"
                     /locus_tag="ACHOTW_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP9842394.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14691..15863
                     /gene="emrA"
                     /locus_tag="ACHOTW_000209"
     CDS             14691..15863
                     /gene="emrA"
                     /locus_tag="ACHOTW_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP9842395.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15880..17418
                     /gene="emrB"
                     /locus_tag="ACHOTW_000210"
     CDS             15880..17418
                     /gene="emrB"
                     /locus_tag="ACHOTW_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP9842396.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17482..17997)
                     /gene="luxS"
                     /locus_tag="ACHOTW_000211"
     CDS             complement(17482..17997)
                     /gene="luxS"
                     /locus_tag="ACHOTW_000211"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9842397.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(18147..19703)
                     /gene="gshA"
                     /locus_tag="ACHOTW_000212"
     CDS             complement(18147..19703)
                     /gene="gshA"
                     /locus_tag="ACHOTW_000212"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP9842398.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(19776..20204)
                     /gene="yqaA"
                     /locus_tag="ACHOTW_000213"
     CDS             complement(19776..20204)
                     /gene="yqaA"
                     /locus_tag="ACHOTW_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFP9842399.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRIFWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(20201..20767)
                     /gene="yqaB"
                     /locus_tag="ACHOTW_000214"
     CDS             complement(20201..20767)
                     /gene="yqaB"
                     /locus_tag="ACHOTW_000214"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP9842400.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(21048..21124)
                     /locus_tag="ACHOTW_000215"
     tRNA            complement(21048..21124)
                     /locus_tag="ACHOTW_000215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21088..21090),aa:Arg,seq:acg)
     gene            complement(21227..21303)
                     /locus_tag="ACHOTW_000216"
     tRNA            complement(21227..21303)
                     /locus_tag="ACHOTW_000216"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21267..21269),aa:Arg,seq:acg)
     gene            complement(21366..21442)
                     /locus_tag="ACHOTW_000217"
     tRNA            complement(21366..21442)
                     /locus_tag="ACHOTW_000217"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21406..21408),aa:Arg,seq:acg)
     gene            complement(21507..21583)
                     /locus_tag="ACHOTW_000218"
     tRNA            complement(21507..21583)
                     /locus_tag="ACHOTW_000218"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21547..21549),aa:Arg,seq:acg)
     gene            complement(21587..21679)
                     /locus_tag="ACHOTW_000219"
     tRNA            complement(21587..21679)
                     /locus_tag="ACHOTW_000219"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21643..21645),aa:Ser,seq:gct)
     gene            complement(21995..22180)
                     /gene="csrA"
                     /locus_tag="ACHOTW_000220"
     CDS             complement(21995..22180)
                     /gene="csrA"
                     /locus_tag="ACHOTW_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP9842401.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(22415..25045)
                     /gene="alaS"
                     /locus_tag="ACHOTW_000221"
     CDS             complement(22415..25045)
                     /gene="alaS"
                     /locus_tag="ACHOTW_000221"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9842402.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(25173..25673)
                     /gene="recX"
                     /locus_tag="ACHOTW_000222"
     CDS             complement(25173..25673)
                     /gene="recX"
                     /locus_tag="ACHOTW_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9842403.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25741..26802)
                     /gene="recA"
                     /locus_tag="ACHOTW_000223"
     CDS             complement(25741..26802)
                     /gene="recA"
                     /locus_tag="ACHOTW_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9842404.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26882..27379)
                     /gene="pncC"
                     /locus_tag="ACHOTW_000224"
     CDS             complement(26882..27379)
                     /gene="pncC"
                     /locus_tag="ACHOTW_000224"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP9842405.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(27524..28609)
                     /gene="mltB"
                     /locus_tag="ACHOTW_000225"
     CDS             complement(27524..28609)
                     /gene="mltB"
                     /locus_tag="ACHOTW_000225"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP9842406.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28864..29426
                     /gene="srlA"
                     /locus_tag="ACHOTW_000226"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             28864..29426
                     /gene="srlA"
                     /locus_tag="ACHOTW_000226"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            29423..30384
                     /gene="srlE"
                     /locus_tag="ACHOTW_000227"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             29423..30384
                     /gene="srlE"
                     /locus_tag="ACHOTW_000227"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            30395..30766
                     /gene="srlB"
                     /locus_tag="ACHOTW_000228"
                     /gene_synonym="gutB"
     CDS             30395..30766
                     /gene="srlB"
                     /locus_tag="ACHOTW_000228"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9842407.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30770..31549
                     /gene="srlD"
                     /locus_tag="ACHOTW_000229"
     CDS             30770..31549
                     /gene="srlD"
                     /locus_tag="ACHOTW_000229"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP9842408.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31655..32014
                     /gene="gutM"
                     /locus_tag="ACHOTW_000230"
     CDS             31655..32014
                     /gene="gutM"
                     /locus_tag="ACHOTW_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9842409.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            32081..32854
                     /gene="srlR"
                     /locus_tag="ACHOTW_000231"
                     /gene_synonym="gutR"
     CDS             32081..32854
                     /gene="srlR"
                     /locus_tag="ACHOTW_000231"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP9842410.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            32847..33812
                     /gene="gutQ"
                     /locus_tag="ACHOTW_000232"
     CDS             32847..33812
                     /gene="gutQ"
                     /locus_tag="ACHOTW_000232"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP9842411.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33809..35323)
                     /gene="norR"
                     /locus_tag="ACHOTW_000233"
     CDS             complement(33809..35323)
                     /gene="norR"
                     /locus_tag="ACHOTW_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP9842412.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            35510..36745
                     /locus_tag="ACHOTW_000234"
     CDS             35510..36745
                     /locus_tag="ACHOTW_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP9842413.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREIARQWALAPLP
                     QSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGTPWSEVPDNFL
                     CPECSLGKDVFDELASEAK"
     gene            36742..37875
                     /gene="norW"
                     /locus_tag="ACHOTW_000235"
     CDS             36742..37875
                     /gene="norW"
                     /locus_tag="ACHOTW_000235"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP9842414.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(38003..40255)
                     /gene="hypF"
                     /locus_tag="ACHOTW_000236"
     CDS             complement(38003..40255)
                     /gene="hypF"
                     /locus_tag="ACHOTW_000236"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP9842415.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(40408..40935)
                     /gene="hydN"
                     /locus_tag="ACHOTW_000237"
     CDS             complement(40408..40935)
                     /gene="hydN"
                     /locus_tag="ACHOTW_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP9842416.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(41084..42094)
                     /gene="ascG"
                     /locus_tag="ACHOTW_000238"
     CDS             complement(41084..42094)
                     /gene="ascG"
                     /locus_tag="ACHOTW_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP9842417.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            42354..43811
                     /gene="ascF"
                     /locus_tag="ACHOTW_000239"
     CDS             42354..43811
                     /gene="ascF"
                     /locus_tag="ACHOTW_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP9842418.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            43820..45244
                     /gene="ascB"
                     /locus_tag="ACHOTW_000240"
     CDS             43820..45244
                     /gene="ascB"
                     /locus_tag="ACHOTW_000240"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP9842419.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(45368..45838)
                     /gene="hycI"
                     /locus_tag="ACHOTW_000241"
     CDS             complement(45368..45838)
                     /gene="hycI"
                     /locus_tag="ACHOTW_000241"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP9842420.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(45831..46241)
                     /gene="hycH"
                     /locus_tag="ACHOTW_000242"
     CDS             complement(45831..46241)
                     /gene="hycH"
                     /locus_tag="ACHOTW_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP9842421.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(46238..47005)
                     /gene="hycG"
                     /locus_tag="ACHOTW_000243"
     CDS             complement(46238..47005)
                     /gene="hycG"
                     /locus_tag="ACHOTW_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP9842422.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(47005..47547)
                     /gene="hycF"
                     /locus_tag="ACHOTW_000244"
     CDS             complement(47005..47547)
                     /gene="hycF"
                     /locus_tag="ACHOTW_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP9842423.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(47557..49266)
                     /gene="hycE"
                     /locus_tag="ACHOTW_000245"
     CDS             complement(47557..49266)
                     /gene="hycE"
                     /locus_tag="ACHOTW_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP9842424.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(49284..50207)
                     /gene="hycD"
                     /locus_tag="ACHOTW_000246"
     CDS             complement(49284..50207)
                     /gene="hycD"
                     /locus_tag="ACHOTW_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP9842425.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(50210..52036)
                     /gene="hycC"
                     /locus_tag="ACHOTW_000247"
     CDS             complement(50210..52036)
                     /gene="hycC"
                     /locus_tag="ACHOTW_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP9842426.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(52033..52644)
                     /gene="hycB"
                     /locus_tag="ACHOTW_000248"
     CDS             complement(52033..52644)
                     /gene="hycB"
                     /locus_tag="ACHOTW_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP9842427.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(52769..53230)
                     /gene="hycA"
                     /locus_tag="ACHOTW_000249"
     CDS             complement(52769..53230)
                     /gene="hycA"
                     /locus_tag="ACHOTW_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP9842428.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            53442..53792
                     /gene="hypA"
                     /locus_tag="ACHOTW_000250"
     CDS             53442..53792
                     /gene="hypA"
                     /locus_tag="ACHOTW_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9842429.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            53796..54668
                     /gene="hypB"
                     /locus_tag="ACHOTW_000251"
     CDS             53796..54668
                     /gene="hypB"
                     /locus_tag="ACHOTW_000251"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP9842430.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            54659..54931
                     /gene="hypC"
                     /locus_tag="ACHOTW_000252"
     CDS             54659..54931
                     /gene="hypC"
                     /locus_tag="ACHOTW_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP9842431.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            54931..56052
                     /gene="hypD"
                     /locus_tag="ACHOTW_000253"
     CDS             54931..56052
                     /gene="hypD"
                     /locus_tag="ACHOTW_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP9842432.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPAVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            56049..57059
                     /gene="hypE"
                     /locus_tag="ACHOTW_000254"
     CDS             56049..57059
                     /gene="hypE"
                     /locus_tag="ACHOTW_000254"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP9842433.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            57133..59211
                     /gene="flhA"
                     /locus_tag="ACHOTW_000255"
     CDS             57133..59211
                     /gene="flhA"
                     /locus_tag="ACHOTW_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP9842434.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(59248..59601)
                     /gene="ygbA"
                     /locus_tag="ACHOTW_000256"
     CDS             complement(59248..59601)
                     /gene="ygbA"
                     /locus_tag="ACHOTW_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP9842435.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(59678..59812)
                     /locus_tag="ACHOTW_000257"
     CDS             complement(59678..59812)
                     /locus_tag="ACHOTW_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9842436.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            59888..62449
                     /gene="mutS"
                     /locus_tag="ACHOTW_000258"
     CDS             59888..62449
                     /gene="mutS"
                     /locus_tag="ACHOTW_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9842437.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            62555..63211
                     /gene="pphB"
                     /locus_tag="ACHOTW_000259"
     CDS             62555..63211
                     /gene="pphB"
                     /locus_tag="ACHOTW_000259"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9842438.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(63252..63488)
                     /locus_tag="ACHOTW_000260"
     CDS             complement(63252..63488)
                     /locus_tag="ACHOTW_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP9842439.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(63499..64926)
                     /locus_tag="ACHOTW_000261"
     CDS             complement(63499..64926)
                     /locus_tag="ACHOTW_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP9842440.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(64926..65519)
                     /locus_tag="ACHOTW_000262"
     CDS             complement(64926..65519)
                     /locus_tag="ACHOTW_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP9842441.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            65666..66073
                     /locus_tag="ACHOTW_000263"
     CDS             65666..66073
                     /locus_tag="ACHOTW_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9842442.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(66193..67185)
                     /gene="rpoS"
                     /locus_tag="ACHOTW_000264"
     CDS             complement(66193..67185)
                     /gene="rpoS"
                     /locus_tag="ACHOTW_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP9842443.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(67248..68342)
                     /gene="nlpD"
                     /locus_tag="ACHOTW_000265"
     CDS             complement(67248..68342)
                     /gene="nlpD"
                     /locus_tag="ACHOTW_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP9842444.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(68527..69153)
                     /gene="pcm"
                     /locus_tag="ACHOTW_000266"
     CDS             complement(68527..69153)
                     /gene="pcm"
                     /locus_tag="ACHOTW_000266"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP9842445.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(69147..69908)
                     /gene="surE"
                     /locus_tag="ACHOTW_000267"
     CDS             complement(69147..69908)
                     /gene="surE"
                     /locus_tag="ACHOTW_000267"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP9842446.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(69889..70938)
                     /gene="truD"
                     /locus_tag="ACHOTW_000268"
     CDS             complement(69889..70938)
                     /gene="truD"
                     /locus_tag="ACHOTW_000268"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP9842447.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(70935..71414)
                     /gene="ispF"
                     /locus_tag="ACHOTW_000269"
     CDS             complement(70935..71414)
                     /gene="ispF"
                     /locus_tag="ACHOTW_000269"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP9842448.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(71414..72124)
                     /gene="ispD"
                     /locus_tag="ACHOTW_000270"
     CDS             complement(71414..72124)
                     /gene="ispD"
                     /locus_tag="ACHOTW_000270"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP9842449.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(72143..72454)
                     /gene="ftsB"
                     /locus_tag="ACHOTW_000271"
     CDS             complement(72143..72454)
                     /gene="ftsB"
                     /locus_tag="ACHOTW_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP9842450.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(72648..72971)
                     /gene="ygbE"
                     /locus_tag="ACHOTW_000272"
     CDS             complement(72648..72971)
                     /gene="ygbE"
                     /locus_tag="ACHOTW_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP9842451.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(73021..73626)
                     /gene="cysC"
                     /locus_tag="ACHOTW_000273"
     CDS             complement(73021..73626)
                     /gene="cysC"
                     /locus_tag="ACHOTW_000273"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP9842452.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(73626..75053)
                     /gene="cysN"
                     /locus_tag="ACHOTW_000274"
     CDS             complement(73626..75053)
                     /gene="cysN"
                     /locus_tag="ACHOTW_000274"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP9842453.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(75055..75963)
                     /gene="cysD"
                     /locus_tag="ACHOTW_000275"
     CDS             complement(75055..75963)
                     /gene="cysD"
                     /locus_tag="ACHOTW_000275"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP9842454.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            76215..77252
                     /gene="iap"
                     /locus_tag="ACHOTW_000276"
     CDS             76215..77252
                     /gene="iap"
                     /locus_tag="ACHOTW_000276"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP9842455.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   77334..77546
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=77395..77424
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacaca"
     gene            complement(77642..77935)
                     /gene="cas2e"
                     /locus_tag="ACHOTW_000277"
     CDS             complement(77642..77935)
                     /gene="cas2e"
                     /locus_tag="ACHOTW_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP9842456.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(77932..78855)
                     /gene="cas1e"
                     /locus_tag="ACHOTW_000278"
     CDS             complement(77932..78855)
                     /gene="cas1e"
                     /locus_tag="ACHOTW_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP9842457.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(78852..79502)
                     /gene="cas6e"
                     /locus_tag="ACHOTW_000279"
     CDS             complement(78852..79502)
                     /gene="cas6e"
                     /locus_tag="ACHOTW_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP9842458.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(79484..80230)
                     /gene="cas5e"
                     /locus_tag="ACHOTW_000280"
     CDS             complement(79484..80230)
                     /gene="cas5e"
                     /locus_tag="ACHOTW_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP9842459.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(80241..81296)
                     /gene="cas7e"
                     /locus_tag="ACHOTW_000281"
     CDS             complement(80241..81296)
                     /gene="cas7e"
                     /locus_tag="ACHOTW_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP9842460.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(81308..81844)
                     /gene="casB"
                     /locus_tag="ACHOTW_000282"
                     /gene_synonym="cse2"
     CDS             complement(81308..81844)
                     /gene="casB"
                     /locus_tag="ACHOTW_000282"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP9842461.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(81841..83403)
                     /gene="casA"
                     /locus_tag="ACHOTW_000283"
                     /gene_synonym="cse1"
     CDS             complement(81841..83403)
                     /gene="casA"
                     /locus_tag="ACHOTW_000283"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP9842462.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(83501..>86150)
                     /gene="cas3"
                     /locus_tag="ACHOTW_000284"
     CDS             complement(83501..>86150)
                     /gene="cas3"
                     /locus_tag="ACHOTW_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFP9842463.1"
                     /translation="FDFWGKTRRGEKEGGDGYHLLCWHSLDVAAMGYLMVKRNCFGLA
                     DYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAPELKIPEGSRKNYEKISH
                     STLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITTGHHGRPPDRIDELDNFL
                     PEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYISATVVLADWTGSSTR
                     FFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFPFIEHPTPLQQKV
                     LDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQGLFFGLPTMATANAMYD
                     RLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAW
                     FANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCI
                     LEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHV
                     TKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQGKCIAWIRNSVDDAIKV
                     HRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQVLE
                     QSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVW
                     DDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVV
                     MPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLPEKLSTRLAEESVSLW
                     LATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRQWCIEQRQDPE
                     MANVILVTDDESCGYSAREGLIGKVD"
BASE COUNT        20427 a        23410 c        22008 g        20305 t
ORIGIN      
        1 tctgctcgct aattccgcaa gcctgacaaa accaaagtga ataaaaatgc aatataacag
       61 aataaaataa aacacccctc actggtgctt tctgctgtta atgtacattt tctggcataa
      121 aaaatgctgt aaatgacccg gtcacgacat acaccgaatt ctctacgctt atagcgttac
      181 ctcaccccca aactttcatt agtgaaatta aaggaaaggt catatccaat tgatttaatt
      241 gaaaataaat catatcggca tgtaaaaatc cacaatgcac aaaaaacgag ccgttacgga
      301 atattttatc tacaaaaact gactaaataa aaatttttca ctaattgatt agtcatagcc
      361 agcgatatac gctatgcgaa aatgcagatg gcaatgagat ccactgcttt catctccatt
      421 aacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc
      481 gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta
      541 ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga ttggtagcga
      601 ttccggtgaa catcttaatt gcatggccat acggtatgta ccgcgatctg tttatgcgcg
      661 cggcacgcaa agttagcccg tcgggctgga taaaaaatct ggcggatatc ctggcttatg
      721 tgacgttcca gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca gactggcatc
      781 agattatggc ggcggtcagt tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg
      841 gctacttcct cgattattgc cgccgactgt ttaaagtcag ccgttaccag caggtaaaag
      901 cctgactctt cttttcgcga ctggcatcgc cagtcgcgtc tttaactggc ttcgccaaat
      961 aaaccattca aataacgttc aagcgcaaca cgcgaactaa agccatgcgg gataccgtaa
     1021 tgctcgtgtc gctcgcctgc taaatagagc ggaaattgct ggtaatgatc gcaagtttta
     1081 atatcggaag cgggttcagc cagtgtcgaa ctgcgttctt ttaatgttgg atgaattacc
     1141 agagcggtac gtcccattcg cgcttcgcga ttaacataga cataattttc cccacggcgg
     1201 taaccatacg ctttctgggt taccacatcg acagtaaaac cgactttttc aagtacgcgc
     1261 gccacctcgt ctggtcgtaa atacatattt gatcctcgtt attatcaacc gcgggcctac
     1321 cttacctgat tgcgcattca caacgctttc agaaaagtcc ataaagccgc gatgaacctc
     1381 agttaagaaa attatggtct acactgaaaa ttacatcgaa ttctaatggg ggatcatatg
     1441 tttaaccgac cgaaccgcaa cgacgttgat gatggcgtgc aggatatcca gaatgatgtc
     1501 aatcaattag ctgacagcct cgaatctgta ttgaagtcct ggggcagcga cgccaaaggg
     1561 gaagctgaag ccgcacgcag taaagctcag gcattgctga aagaaaccag ggcgcgaatg
     1621 catggtcgta ctcgcgtcca gcaagccgcg cgcgatgccg taggctgcgc ggattctttt
     1681 gttcgtgaaa gaccctggtg tagtgtggga actgctgctg cggtaggtat ttttatcggt
     1741 gcgttgttga gcatgcgtaa atcgtagtga gacgatgaca agcagtagtc tgacttctga
     1801 aaagtactca gtgcgtcaac gccttgtccg cctacaggcc tgaaaaacat agcgcatcag
     1861 gggattttgc cctggtcacc agtcttatag gaccccgaag cctggcttcg gggttattaa
     1921 tttaaagcac tgcgcaattg ctgtgctacc tgtcgcgcca tactttcgct aacgatattc
     1981 gtaaacgaga gcaataatcc tccgtcctcg cttgagcgga tacgccagtc gctaagtgcc
     2041 tgtacggcaa gaccagcagc attggcttta tgcacgaatg tcacgtcatt acctgctagt
     2101 ttgatcacca tctggatacc gcctttttgc ggcacaacct gaaatccctg ctgcgtaagt
     2161 gcctgctcaa tccactgccg tcgctgggca taatgctggc gcattttctt cagatgccgc
     2221 cagaaatgcc cctcacggat gaaatctgcc agtgtgttct gccacagtac gggtacagca
     2281 catggttcca gtgacgcctg ctgccggaat tgcgaaatct gctccaccgg caccaccagc
     2341 cacgcacagc gcaatgcagg aaatagcgct ttgctgaatg ttccggcata aatcacccgc
     2401 tgcggcgcgt cgagactttt tagtgccggt aacggcttac cgtgatagcg aaactcactg
     2461 tcgtaatcat cttcaataat ccatgcctga ctacgctctg cccattccag tatctgatac
     2521 ctacgcgcta aagagagtgc cacacccagc gggctttggt gtgccggagt aatcagggca
     2581 aaacgcgcat caggataatt ttgtattccg cttgtgacat ccagtccgtt gtcatcaacc
     2641 ggcacaggta aaatttccac accgtggcga gtgacaatcg ggcgaatcag cggaaagccg
     2701 ggatcttcca tccacatccc gtctcccggt tgcgccagag cgtgcagaat taaagccatt
     2761 gaggccgcat aaccgtgagt gataaagacc tgctctggct gacaatcaat cccacgtgaa
     2821 acgcgtaaat aatcaacaat agcctcgcgc aaggccgcct cgccgcagac atcgcctaat
     2881 gccaaatcaa agcgcgtctg ggtacgcaga cggcggccca tcacccgcgc ccacaattca
     2941 cgcggaaaaa gatcaagtgc aggtagcccc atctgaaaag gcagcggctg ctgactttca
     3001 ccagcaaaag ccacgacatc cacagcagac tgttgcggat gtatctgctc actgacaaag
     3061 gtacccgcct gtccccgcct gaccagccat ccctgcgcca ccagctcagc ataagcattt
     3121 tcgacagtgg atcgagagac acccagctcc tgcgaccagg tacggctcga aggtagcctt
     3181 gcgccaggtt tcagttttcc ttgctcgata accgttttta actgacgggc gatatcctga
     3241 taacgtggca tatggtctgc ttttttaggt aatttatggc tctctttagc agtccattat
     3301 agtgctacat tgcgggcgtt aataagagga gaaactgtat gactacatta cgtcagcctt
     3361 actacgaact tagcccggca gtgtataacg cactggtgca ggccaaaacg gcactggaaa
     3421 atagcacgct ggataccacg ttgatggagt taatttattt gcgcgtctcg caaatcaacg
     3481 gctgcgcatt ttgtctggag atgcacagca aagcattgcg caaatccggt gtgccacaac
     3541 acaaactgga cgccctggca ggctggcgcg taagccatca ttttgatgaa cgcgagcgag
     3601 cggcgctggc atgggcggaa tctgtgaccg atattgccag aacccatgcg gaagacgagg
     3661 tttatcagcc tttgcttgag catttcagcg cagcagaaat cagcgactta acgtttgcca
     3721 tcgggctgat gaattgtttt aaccgtctgg ccgtttccat gcggatgtaa tttgctgttg
     3781 ccgggtatgc gcccggcaaa aaaacacaaa atcccctacc ccgttgagcc attcaccggt
     3841 ggcgtaatca atctaaaatt accatatctt gtgtggttga atcttttttc aactacatct
     3901 agtatctctg tatcaacaga gagacaaccc gacgcgtatc atcgcgccgt atcttcattt
     3961 taaacggaaa tacgaatcat gcgcattact atttacactc gtaacgattg cgttcagtgc
     4021 cacgccacca aacgggcgat ggaaaaccgg ggctttgatt ttgaaatgat taatgtcgat
     4081 cgcgttcctg aagcggcaga agcgttgcgt gctcagggct ttcgtcagtt gccggtagtg
     4141 attgctggcg atcttagctg gtctggtttc cgtccggaca tgattaaccg tctgcatcca
     4201 gcgccacacg cggccagtgc atgagccagc tcgtctactt ctccagcagc tccgaaaaca
     4261 cgcagcgttt tatcgaacgt ttaggtctgc ccgcggtgcg catcccgctc aatgagcggg
     4321 aacggattca ggtagacgag ccttacatcc tgatcgtgcc ctcttacggc ggcggcggta
     4381 cggctggcgc ggtgccacga caggtaattc gctttttaaa cgacgagcac aaccgggcgt
     4441 tgcttcgcgg cgttattgct tcgggtaatc gcaactttgg tgaggcgtat ggccgcgccg
     4501 gagatgtgat tgcccggaaa tgcggcgtgc cgtggctgta ccgttttgaa ctcatgggta
     4561 cgcaaagcga tatcgaaaac gttcgtaaag gagtaaccga attttggcaa cgacaaccgc
     4621 agaatgcctg acgcagaaaa cgatggatta ccacgcgctg aatgcgatgc ttaacctcta
     4681 cgatagcgca ggtcgcattc agttcgataa agaccgccag gccgttgacg cctttatggc
     4741 gacgcatgtg cgtccgaaca gtgtgacctt cagtagccag cagcagcgcc tgaactggct
     4801 ggtcaacgaa ggttactatg atgaaagcgt tcttaatcgc tactctcgcg actttgtcat
     4861 tacgctgttt gcccacgcac acaccagcgg ttttcgtttc cagacattcc tcggggcatg
     4921 gaagttttac accagctata cgttgaagac attcgacggt aaacgttatc tggaagattt
     4981 tgccgatcga gtcacgatgg tggcgctgac gctggcacaa ggcgatgaga cgctggcgtt
     5041 gcaactgaca gatgaaatgc tgtcaggacg ctttcagcca gccacgccaa cattcctcaa
     5101 ctgcggtaag cagcagcgcg gcgaactggt ttcctgtttt ttgctgcgta ttgaagacaa
     5161 tatggagtcg attggtcggg cggtaaattc cgcactgcaa ctgtcgaaac gcggcggcgg
     5221 cgtagcattt ttgctgtcga atctgcggga agcgggcgcg ccaattaaac gtattgaaaa
     5281 tcaatcttct ggcgtaattc cggtgatgaa aatgctggaa gacgcatttt cctatgccaa
     5341 ccaactcggc gctcgtcagg gggctggtgc agtctattta catgctcatc atcccgatat
     5401 tctgcgtttt ctcgacacga aacgggaaaa tgccgacgaa aaaatccgca ttaaaacact
     5461 gtcgcttggc gtggtgatcc cggatatcac tttccatctg gcaaaagaga atgcgcagat
     5521 ggcgctgttt tcgccttatg acgtagagcg agtttatggc aagccgtttg ccgatgtggc
     5581 catcagccaa cactatgacg aactggttgc cgatgaacgc attcgcaaaa aatacctcaa
     5641 cgcccgtgat ttcttccagc gactggcaga aatccagttt gagtccggct atccctacat
     5701 catgtatgaa gacacggtaa accgtgctaa ccctgtcgcc gggcgcataa atatgagtaa
     5761 tctctgctca gaaattttgc aggttaacag cgcctcagag tatgacgaga atctcgacta
     5821 tgcccgcaca ggccatgata tctcctgcaa tttaggttcg ttgaatattg cgcacaccat
     5881 ggattccccc gattttgccc gcacggtaga gactgccgtg cgcggtttaa cggcagtatc
     5941 agatatgagt catatccgca gcgtgccgtc catcgaagcc ggaaatgccg cctcgcacgc
     6001 catcggactg gggcagatga atttacacgg ctatctggcg cgagaaggca tcgcttatgg
     6061 ttcgccggaa gcactggatt tcaccaatct ctatttctat gccatcacct ggcacgcact
     6121 gcgtacctcg atgttgctgg cacgcgaacg cggtgaaacc ttcgccgggt tcaaacagtc
     6181 acgctatgcc agtggtgaat attttagcca atatctgcaa gggaactggc agccgaaaac
     6241 ggggaaagtt ggcgaactgt ttgcccgtag cggtattacg ttacctaccc gtgagatgtg
     6301 ggcgcaactg cgcgacgacg tgatgcgcta cggcatttac aaccagaatc ttcaggcggt
     6361 gccgccaaca ggttctatct cttatatcaa ccatgctacg tcgagtattc atccgattgt
     6421 ggcgaaagta gagatacgca aagagggcaa aacaggacgc gtttactacc ctgccccgtt
     6481 tatgactaac gagaatctgg cgctgtatca ggacgcttac gaaattggcg cagaaaagat
     6541 catcgacacc tacgcggaag cgactcgcca tgtcgatcag gggctgtcgc tgacgctgtt
     6601 tttccccgat accgccacca ctcgcgatat caacaaagcg cagatttacg cctggcgcaa
     6661 aggtatcaaa acgctctatt acatccggct gcgtcagatg gcgctggaag gcactgaaat
     6721 tgaaggctgc gtctcctgtg cactttaagg aatatctatg aaactctcac gtatcagcgc
     6781 catcaactgg aacaagatat ctgacgataa agatctggag gtgtggaatc gcctgaccag
     6841 caatttctgg ctaccagaaa aggtgccgct gtcgaacgat attcctgcct ggcagacatt
     6901 aactgtcgta gaacaacaac tgacgatgcg cgtttttact ggcctgacgc tgctcgacac
     6961 gctgcaaaat gttatcggcg cgccttcgct gatgcccgat gcactcacgc ctcatgaaga
     7021 agcggtatta tcgaatatca gctttatgga agcggttcat gcccgctctt acagttcgat
     7081 tttctcgacg ctgtgccaga ccaaagatgt cgatgccgcc tacgcctgga gtgaagaaaa
     7141 cgcgccgttg cagcgaaaag cacagattat tcagcaacat taccgcggtg atgatccgct
     7201 gaaaaagaaa atcgccagtg tgtttcttga atcttttttg ttctattccg gtttctggct
     7261 gccgatgtat ttttccagcc gcggaaagct gaccaatacc gcggacctga tccgtctgat
     7321 tatccgcgat gaagcagtcc acggttacta cataggctat aaatatcaga aaaacatgga
     7381 aaagatatct ctgggacaac gtgaagagtt gaagagtttc gccttcgatt tgttgctgga
     7441 actctacgac aacgagttgc aatacaccga tgagctgtac gccgaaaccc cgtgggctga
     7501 cgatgtgaaa gcgtttctct gttacaacgc caataaggct ttgatgaatc tgggctacga
     7561 accgttattt cccgcagaaa tggcggaagt gaatccggca atcctcgccg cgctttcgcc
     7621 gaatgccgat gaaaatcacg atttcttttc cggttcaggc tcctcttatg tgatggggaa
     7681 agcggttgaa acagaatatg aagactggaa tttctgaggg tgttattttc aaaaatatca
     7741 ctacccgcga cagggaaata attcccgcca aatagctttt tatcacgcaa atattttgtg
     7801 gtgatctaca ctgatactct gttgcattat tcgcctgaaa ccacaatatt caggcgtttt
     7861 ttcgctatct ttgacaaaaa atatcaactt ttctcgattt gctctcagcc cttatatcac
     7921 gggaaattcc ggcgatttgc tcgcatcaat attcatgcca catttgccat caggggttgc
     7981 ctcagattct cagtatgtta gggtagaaaa aagtgactat ttccattggg taatatatcg
     8041 acataaacaa ataacaggaa tctttctatt gcatggcaat taaattagaa attaaaaatc
     8101 tttataaaat atttggtgag catccacagc gagcgttcaa atatatcgaa caaggacttt
     8161 caaaagaaca aattctggaa aaaactgggc tatcgcttgg cgtaaaagac gccagtctgg
     8221 ccattgaaga aggcgagata tttgtcatca tgggattatc cggctcgggt aaatccacaa
     8281 tggtacgcct tctcaatcgc ctgattgaac ccacccgcgg gcaagtactg attgatggtg
     8341 tggatattgc caaaatatcc gacgccgaac tccgtgaggt gcgcagaaaa aagattgcga
     8401 tggtcttcca gtcctttgcc ttaatgccgc atatgaccgt gctggacaat actgcgtttg
     8461 gtatggaatt ggccggaatt aatgccgaag aacgccggga aaaagccctt gatgcactgc
     8521 gtcaggtcgg gctggaaaat tatgcccaca gctacccgaa tgaactctct ggcgggatgc
     8581 gtcaacgtgt gggattagcc cgcgcgttag cgattaatcc ggatatttta ttaatggatg
     8641 aagccttctc ggcgctcgat ccattaattc gcaccgagat gcaggatgag ctggtaaaat
     8701 tacaggcgaa acatcagcgc accattgtct ttatttccca cgatctcgat gaagccatgc
     8761 gtattggcga ccgaattgcc attatgcaaa atggtgaagt ggtacaggtc ggcacaccgg
     8821 atgaaattat caataatccg gcgaatgatt atgtccgtac cttcttccgt ggcgttgata
     8881 ttagtcaggt attcagtgcg aaagatattg cccgccggac accgaatggc ttaattcgta
     8941 aaacccctgg cttcggccca cgttcggcac tgaaattatt gcaggatgaa gatcgtgaat
     9001 atggctacgt tatcgaacgc ggtaataagt ttgtcggcgc agtctccatc gattcgctta
     9061 aaaccgcgtt aacgcagcag caaggtcttg atgcggcgct gattgatgcg ccgttagcag
     9121 tcgatgcaca aacgcctctt agcgagttgc tctctcatgt cggacaggct ccctgtgcgg
     9181 tgcccgtggt cgacgaggac caacagtatg tcggcatcat ttcgaaagga atgctgctgc
     9241 gcgctttaga tcgtgagggg gtaaataatg gctgatcaaa ataatccgtg ggataccacg
     9301 ccagcggcgg acagtgccgc gcaatccgca gacgcctggg gtacaccggc gactgcaccg
     9361 actgacggcg gtggtgctga ctggctgacc agtacgcctg cgccaaacgt cgagcatttt
     9421 aatattctcg atccgttcca taaaacgctg atcccgctcg acagttgggt cactgaaggg
     9481 atcgactggg tcgttaccca tttccgtccc gtcttccagg gcgtgcgcgt tccggttgat
     9541 tatatcctca acggtttcca gcaattgctg ctgggtatgc ccgcaccggt ggcgattatc
     9601 gttttcgctc tcatcgcctg gcagatttcc ggggtcggaa tgggcgtggc gacgctggtt
     9661 tcgctgattg ccatcggcgc aatcggtgcc tggtcgcagg caatggttac tctggcgctg
     9721 gtgttaaccg ccctgctgtt ctgtatcgtc atcggtttgc cgttggggat ctggctggcg
     9781 agaagtccgc gagcggcgaa aattattcgt ccactgcttg atgccatgca gaccacgcca
     9841 gcgtttgttt atctggtgcc aatcgtcatg ctatttggta tcggtaacgt gccgggcgtg
     9901 gtggtgacga tcatctttgc tctgccgccg attatccgtc tgaccattct ggggattaac
     9961 caggttccgg cggatctgat tgaagcctcg cgctcattcg gtgccagccc gcgccagatg
    10021 ctgttcaaag ttcagttacc gctggcgatg ccgaccatta tggcgggcgt taaccagacg
    10081 ctgatgctgg ccctttctat ggtggtcatc gcctcgatga ttgccgtcgg cgggttgggt
    10141 cagatggtac ttcgtggtat cggtcgtctg gatatggggc ttgccaccgt tggcggcgtc
    10201 gggattgtga tcctcgccat tatcctcgat cgtctgacgc aggccgttgg gcgcgactca
    10261 cgcagtcgcg gcaaccgtcg ctggtacacc actggccctg tcggtctgct gacccgccca
    10321 ttcattaagt aactctgcac ttgcccggtg acgccgggca tatcaccctg ccaaaaaaag
    10381 gaataacaat gcgacatagc gtactttttg cgacagcgtt tgccacgctt atctctacac
    10441 aaacttttgc tgccgacctg ccgggcaaag gcattactgt taatccggtt cagagcacca
    10501 tcaccgaaga aactttccag acgctgctgg tcagccgcgc gctggagaaa ttaggttata
    10561 ccgtcaataa acccagcgaa gtggattaca acgttggcta cacctcgctt gcttccggcg
    10621 atgcaacctt caccgccgtg aactggacgc cactgcatga caacatgtac gaagctgccg
    10681 gtggcgataa gaaattttat cgtgaagggg tatttgttaa cggcgcggca cagggttacc
    10741 tgatcgataa gaaaaccgcc gaccagtaca aaatcaccaa catcgcacaa ctgaaagatc
    10801 cgaagatcgc caaactgttc gataccaacg gcgacggaaa agcggattta accggttgta
    10861 accctggctg gggctgcgaa ggtgcgatca accaccagct tgccgcgtat gaactgacca
    10921 ataccgtgac gcataatcag gggaactacg cggcgatgat ggccgacacc atcagtcgtt
    10981 acaaagaggg caaaccggtg ttttactaca cctggacgcc gtactgggtg agtaatgagc
    11041 tgaagccagg caaagatgtg gtctggttgc aggtgccgtt ctctgcactg ccgggcgata
    11101 aaaatgccga taccaaactg ccgaatggcg cgaattacgg cttcccggtc agcaccatgc
    11161 atatcgttgc caataaagcc tgggccgaga aaaacccggc agcagcgaaa ctgtttgcca
    11221 ttatgcagtt gccagtggca gatattaacg cccagaacgc cattatgcat gacggcaaag
    11281 cctcagaagg cgatattcag ggccacgttg atggctggat caaagcccac cagcaacagt
    11341 tcgatggctg ggtgaatgag gcgttggcag cgcagaagta atttttattc gggcggataa
    11401 ggcgtttacg ccgcatccgc cgttctgtac acaatgcctg atgcgacgct ggcgcgtctt
    11461 atcatgccta caaaccacac cgcacatttc aacaatctat ctttcatccc atattcatca
    11521 acatccgcta ttattgattt ccagcttaat catcacctga tgaacaaaaa taatgaccaa
    11581 acctaatcat gagcttagcc cggcgctaat cgtgctgatg tctatcgcca ccggtctggc
    11641 ggtcgccagc aactattacg cccagccatt gctcgacacc atcgcgcgta acttttccct
    11701 ttccgccagt tcggcaggct ttattgttac cgccgcgcag ttgggctatg ccgccggtct
    11761 gctgtttctt gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac
    11821 cttactggca gcgggcggta tgttaatcac tgccagcagt cagtcactgg cgatgatgat
    11881 cctcggtacg gcattaaccg gtttattctc agtcgtggca caaattctgg ttccgctggc
    11941 agcgacgctg gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta tgagcggtct
    12001 gctgttgggg atattgctgg cacgaaccgt tgccgggtta ctggcaaacc tcggcggctg
    12061 gcgcaccgtc ttttgggttg cctcggtgtt aatggctctg atggcgctgg cattatggcg
    12121 tggtctgcca caaatgaaat cagaaaccca cctcaactac ccacagttgt tgggttccgt
    12181 tttcagcatg tttatcagca ataagatcct gcgcacccgc gcgttgctgg gctgcctgac
    12241 ctttgccaac ttcagcattc tctggacctc aatggccttt ttgcttgccg ctccaccttt
    12301 taactacagc gatggtgtaa ttggtctgtt tgggcttgcg ggagctgccg gagcgttggg
    12361 cgctcgtccg gcgggcggtt ttgccgacaa gggcaaatcg caccacacca caactttcgg
    12421 tctgcttctg ctgttacttt catggctggc aatctggttt ggtcacactt ccgtactggc
    12481 gttgattatc ggcattctgg tgctggacct caccgtacag ggcgtgcata tcactaacca
    12541 gacggtaatt tatcgaatac atcctgatgc gcgtaatcgc ctgaccgcag gttacatgac
    12601 cagctacttt attggcggtg ccgccggttc gctaatttca gcctcagcct ggcaacatgg
    12661 cggttgggct ggcgtttgtc tggctggcgc gacgattgcc ctggttaact tactggtctg
    12721 gtggcgaggt tttcatcgtc aggaagccgc aaattaagca atcagcaacc gttttcgtgg
    12781 ctttacactt ataagggtgt taagaagccc atcagtctga taaggttaag atattcattc
    12841 agtctattta taatattaac aatcgttaag cgtacactct atggaaagcc ctactccaca
    12901 gcctgctcct ggttcggcga ccttcatgga aggatgcaaa gacagtttac cgattgttat
    12961 tagttatatc ccggtggcct ttgcgttcgg tctgaatgcg acccgtctgg gattctctcc
    13021 tctcgaaagc gtttttttct cctgcatcat ttatgcaggc gcgagccagt tcgtcattac
    13081 cgcgatgctg gcagcaggga gtagtttgtg ggttgctgca ctgaccgtca tggcaatgga
    13141 tgttcgccat gttttgtatg gcccgtcact gcgtagccgt attattcagc gtctgcaaaa
    13201 atcgaaaacc gccctatggg cgtttggcct gacggatgag gtttttgccg ccgccaccgc
    13261 aaaactggta cgcaataatc gccgctggag cgagaactgg atgatcggca ttgccttcag
    13321 ttcatggtca tcgtgggtat ttggtacggt aataggggca ttctccggca gcggcttgct
    13381 gcaaggttat cccgccgttg aagctgcatt aggttttatg cttccggcac tctttatgag
    13441 tttcctgctc gcctcttttc agcgcaaaca atctctttgc gttaccgcag cgttagttgg
    13501 tgcccttgca ggcgtaacgc tattttctat tcccgtcgcc attctggcag gcattgtctg
    13561 tggctgcctc actgcgttaa tccaggcatt ctggcaagga gcgcccgatg agctatgagg
    13621 ttctgctgct tgggttactg gttggcgcgg cgaattattg cttccgctat ttgccgctgc
    13681 gcctgcgtgt gggtaatgcc cgcccaacca aacgtggcgc ggtaggtatt ttgctcgaca
    13741 ccattggcat cgcctcgata tgcgctctgc tggttgtctc taccgcacca gaagtgatgc
    13801 acgatacacg ccgtttcgtg cccacgctgg tcggcttcgc ggtactgggt gccagtttct
    13861 ataaaacacg cagcattatc atcccaacac tgcttagtgc gctggcctat gggctcgcct
    13921 ggaaagtgat ggcgattata taacccacaa gaatcatttt tctaaaacaa tacatttact
    13981 ttatttgtca ctgtcgttac tatatcggct gaaattaatg aggtcatacc caaatggata
    14041 gttcgtttac gcccattgaa caaatgctaa aatttcgcgc cagccgccac gaagattttc
    14101 cttatcagga gatccttctg actcgtcttt gcatgcatat gcaaagcaag ctgctggaga
    14161 accgcaataa aatgctgaag gctcagggga ttaacgagac gttgtttatg gcgttgatta
    14221 cgctggagtc tcaggaaaac cacagtattc agccttctga attaagttgt gctcttggat
    14281 catcccgtac caacgcgacg cgtattgccg atgaactgga aaaacgcggt tggatcgaac
    14341 gtcgtgaaag cgataacgat cgccgctgcc tgcatctgca attaacggaa aaaggtcacg
    14401 agtttttgcg cgaggtttta ccaccgcagc ataactgcct gcatcaactc tggtccgcgc
    14461 tcagcacaac agaaaaagac cagctcgagc aaatcacccg caaattgctc tcccgtctcg
    14521 accagatgga acaagacggt gtggttctcg aagcgatgag ctaacgcgtc atctcgctca
    14581 aaaatccaga tttataaaag aaaaaatgac cggccagcat cgcaacatgc tggccttttt
    14641 ggcaagcagg tcggctcagc cgatgagtta agaagatcgt ggagaacaat atgagcgcaa
    14701 atgcggagac tcaaaccccg cagcaaccgg taaagaagag cggcaaacgt aagcgtctgc
    14761 tcctccttct caccttgctc tttataatta ttgccgtagc gatagggatt tattggtttt
    14821 tggtactgcg tcacttcgaa gaaaccgatg acgcatacgt ggcagggaat caaattcaaa
    14881 ttatgtctca ggtgtctggc agcgtgacga aagtctgggc cgataacacc gattttgtaa
    14941 aagaaggcga cgtgctggtc actctcgacc cgacagatgc tcgccaggcg tttgaaaaag
    15001 ccaaaactgc actggcttcc agcgttcgcc aaacccacca gcagatgatt aacagcaagc
    15061 agttgcaggc gaatattgag gtgcaaaaaa tcgccctcgc gaaagcacaa agcgactaca
    15121 accgccgtgt gccgctgggc aatgccaacc tgattggtcg cgaagagctg caacacgccc
    15181 gcgacgccgt taccagtgcc caggcgcaac tggacgtcgc gattcaacaa tacaatgcca
    15241 atcaggcgat gattctgggg actaaactgg aagatcagcc tgccgtgcaa caggctgcca
    15301 ccgaagtacg taacgcctgg ctggcgctgg agcgtactcg tattgtcagt ccgatgaccg
    15361 gttatgtctc ccgccgcgcg gtacagcctg gggcgcaaat tagcccaacg acgccgctga
    15421 tggcggtcgt tccagccacc aatatgtggg tggatgccaa ctttaaagag acgcagattg
    15481 ccaatatgcg tatcggtcag ccggtcacta tcaccaccga tatttacggc gatgatgtga
    15541 aatacaccgg taaagtggtt ggtctggata tgggcacagg tagcgcgttc tcactgcttc
    15601 cggcgcaaaa tgcgaccggt aactggatca aagtcgttca gcgtctgcct gtgcgtatcg
    15661 aactggacca gaaacagctg gagcaatacc cgctgcgtat cggtttatcc acgctggtga
    15721 gcgtcaatac cactaaccgt gacggtcagg tactggcaaa taaagtacgt tccactccgg
    15781 tagcggtaag caccgcgcgt gaaatcagcc tggcacctgt caataaactg atcgacgata
    15841 tcgtaaaagc taacgctggc taatccagag gtgcgtgtga tgcaacagca aaaaccgctg
    15901 gaaggcgcgc aactggtcat tatgacgatt gcgctgtcac tagcgacatt catgcaggtg
    15961 ctggactcca ccattgctaa cgtggcgatc cccactatcg ccgggaatct tggctcatcg
    16021 ctcagccagg gaacgtgggt aatcacttct ttcggggtgg cgaatgccat ctcgatcccg
    16081 cttaccggct ggctggcaaa gcgcgtcggg gaagtgaaac tgttcctctg gtcaaccatc
    16141 gcctttgcta ttgcgtcgtg ggcgtgtggt gtctccagca gcctgaatat gctgatcttc
    16201 ttccgcgtga ttcaggggat tgtcgccggg ccgttgatcc cgctttcgca aagtctattg
    16261 ctgaataatt acccgccagc caaacgctca atcgcgctgg cgttgtggtc gatgacggtg
    16321 attgtcgcgc caatttgcgg cccaatcctc ggcggatata tcagcgataa ttaccactgg
    16381 ggctggatat tcttcatcaa cgtgccgatt ggcgtggcgg tggtgttgat gacactgcaa
    16441 actctgcgcg gacgtgaaac ccgcaccgaa cggcggcgga ttgatgccgt ggggctggca
    16501 ctgctggtta ttggtatcgg cagcctgcag attatgctcg accgcggtaa agagctggac
    16561 tggttttcat cacaggaaat tatcatcctt accgtggtgg cggtggtggc tatctgcttc
    16621 ctgatcgtct gggagctgac cgacgataac ccgatagtcg atctgtcgtt gtttaagtcg
    16681 cgcaacttca ccatcggctg tttgtgtatc agcctcgcgt atatgctcta cttcggcgcg
    16741 attgttctgc tgccgcagtt gttgcaggag gtctacggtt acacggcgac ctgggcaggt
    16801 ttggcctctg cgccggtagg gattattcca gtgatcctgt cgccgattat cggccgcttc
    16861 gcgcataaac tggatatgcg ccgactggta accttcagct ttattatgta tgccgtctgc
    16921 ttctactggc gtgcctatac ctttgaacca ggtatggatt ttggcgcgtc ggcctggccg
    16981 cagtttatcc aggggtttgc ggtggcctgc ttctttatgc cgctgaccac cattacgctg
    17041 tctggtttgc caccggaacg actggcggcg gcatcgagcc tctccaactt tacgcgtacg
    17101 ctggcggggt ctatcggcac gtcgataacc acaaccatgt ggaccaaccg cgagtcgatg
    17161 caccatgcgc agttgactga gtcggtaaac ccgttcaacc cgaatgccca ggcgatgtac
    17221 agtcaactgg aagggcttgg gatgacacaa cagcaggcat caggctggat tgcccagcag
    17281 atcaccaatc aggggctgat tatttccgcc aatgagatct tctggatgtc ggccgggata
    17341 ttcctcgtct tgctggggct ggtgtggttt gctaaaccgc cctttggcgc aggtggcggc
    17401 ggaggcggtg cgcactaagt acaactaagc cagttcattt gaactggctt ttttcaatta
    17461 attgtgaaga tagtttactg actaaatgtg cagttcctgc aacttctctt tcggcagcgc
    17521 cagttcttcg ttgctgttga tgcgcacgtc acgttccaga atgctacgcg caatatcctg
    17581 cgcttcctgc aacgagtgca tctggtaagt gccacactgg tagacgttca gctccggaat
    17641 ctgattctga tcctgcactt tcagcacgtc ttccattgcc gctttccaga catcagcaac
    17701 acgctgctca tctggcgtac caatcagact catataaaaa ccggtgcggc agcccattgg
    17761 cgagatatcg ataatctcta caccattacc gttaagatgg ttacgcataa aaccagcaaa
    17821 caggtgctcc agggtatgga tccctctttc tggcatcact tctttgttcg gcacgcagaa
    17881 gcgcagatcg aacacggtga ttgcgtcgcc atgcggggtg ttcattgttt tcgccacccg
    17941 aactgcaggc gcttccatcc gggtatgatc gactgtgaag ctatctaaca acggcattta
    18001 gccacctccg gtaatttttt taaaaatttt ctgaactctt tcttcccagg cgagtctgag
    18061 tatatgaaag acgcgcattt gttatcatca tccctgaatt cagagatgaa attttggcca
    18121 ctcatgagtg gcctttttct tttctgtcag gcgtgttttt ccagccacac cgcaaacggt
    18181 tcggtatctg cggcttccat ttcctgctga cggcgttcag atgcctcgcg ctcggctaca
    18241 aaatcctctt cgcgcagaat ttccagcggc tcttcacgca gcagattacg gtacgcttca
    18301 gcaaacgctt tgcctgttcc gccaatacca gtatcaatca tagaccttaa gatacgggca
    18361 gagaaagtca gatcgggatt atcgaagcag gcaaccagtt catcacacac tttctgatac
    18421 gcttcgccgc cgttaatgct atccagcgtt tgcgcgacgc gtttcagatc gcggaacaga
    18481 tctttaccca cctgcggtaa cgggaactgt gcggtttcgc agccgatacc cagcgtcaga
    18541 cccggtttgc gaccttcgag gatcacccgg ttccagttaa cgcgtgtaca ggcaagttcg
    18601 ctactgctca tttccggtgc atcagccagc gcacaccaga ccataaacag gtcgaggaat
    18661 cgcacctgct gttcatctac accaatcggc gagaacgggt tgatgtccag cgaacgcact
    18721 tcaatatatt caatgccgcc acgtaacagc gcatcagaag gcgactcgcc gctgcgggta
    18781 acgcgttttg gacgaatcgg cgcgtacagt tcgttttcaa tctgcagcac gttgctgttg
    18841 atttgcagcc tcttaccgtc tttatcaata ccaatcttcg cgtactcttc cgatggcgtt
    18901 ttgattgcct gtttcaggcc cgctacgtac tcgtaaagat cgttgaaggt aataccaaga
    18961 ttgctttgcg atttattggt atagccgaga tcgctcaaac gaagagaggt cgcatacggc
    19021 aggtaataca taccgcactc ggttttctca aacggcagcg acgttggttt accttgcagg
    19081 aatgaagagc atattgccgg agacgcgcca aacagataag gaatgaccca accgaagcga
    19141 tagtagttgc gaataacgcg gaaatagccc gcagagattt tctctttagc atcagcgccc
    19201 gaaatatcgc cacacttcgc ttgccagaat gccataggca aagagaagtt gtaatgcacg
    19261 ccggagatgg tttgcatcag cgcgccgtag cgatttttca gcccttcacg gtacagcgtt
    19321 ttaaagcgtc cggtgttaga agtgccgtac tgcgccagtt cgatgtcctg accttctgcg
    19381 atgtagcatg gcatacttaa cggccacatc cgctcatcgc ccatattgcg cgccgtataa
    19441 cgatgcagat cgcgcataaa ggtcagcata tgttcaatat caccatccac tggtgtaatg
    19501 aattccagca atgcttccgc aaaatcggta gtaatccatt tatgcgtcag tgcggaacct
    19561 aatgcttcag gatgacctgt tgttgccagt gtgccatcag cattaacacg caaagtttcg
    19621 cgctccagcc cacgctgtat cccctttaac gcctgaggat gtttttccag ccaggccagc
    19681 gcctgtgata cgtccgggat caaattgacc tcccgcctgt caaaatcgtt ttaattagca
    19741 taactgtaat ggtgaccata tgtgcaggcc tacaattagt gccaccacat catgccctga
    19801 acggtcgctg ctgcaactgc aacatagcgt aacgctttac caaggcacaa aaaaaagatt
    19861 accggccccc agaagatgcg catccatccc gctaacagac acagtaaatc gccaaccacg
    19921 ggcatccagc ttaataatag cgtgactgca ccatagcgtt tcagccagcc ggtagctttc
    19981 tcttgccagc gcgatgtttt acgcaatgga cagaaacgcc caaggataac gttagttaac
    20041 cctccaaggc tattacccat tgttgctgtt aaaactaaaa cccagggatg actgatccca
    20101 gaaagcaaca ttgccaccag cacgacttcg gagttgccgg gtaatagtgt agcgctgaga
    20161 aaactactgg caaacaacga gaaaagcgat aacgcttcac tcacagcaag cgaacatcca
    20221 cagcgtccat gcctgctgca cgggccgcct gaataccgaa atcggcatct tcaaagacca
    20281 cacactgcgt cggttgcacg cccatacgct gcgcgcacaa caaaaatgtg tctggcgcgg
    20341 gtttatggtg tttgacgtga tcggcagcga cgacggcgtc aaaataacgg cgtaatccca
    20401 ggtgagccag caatgcctca gcgatggcgc tttcactccc cgttcctaca gccattgggc
    20461 gacgaccatg ccaacttttc accacttcaa caagaggaag cggctcgaca ctatccagca
    20521 gcatacttct tactgcttct gttttttcac gcgctaacgc atgcgggtcg agatcggcct
    20581 gattcagctc aataattgcc tgagcaatac gccaggtggg cgatccatta agcgcaatca
    20641 tcgcctgaac atcgtactga agaccgtagt gccctaatac ttcgcgccac gctttacggt
    20701 gcgtaggctc cgtatccagg attgtgccat ccatatcaaa aattaaacct gcataacgct
    20761 cgtacatggt cttctcgcaa gtcaaaaaat atgacgctac tttagcgtaa ttgcttgatt
    20821 ttgtcgctga tggcgaagga gaagaaaatt caggaaatgg ataaagtagt tatttagaaa
    20881 tgagatattt ttgaaggaaa ttttttggag aagatggtgc atccgggagg atgactcact
    20941 ggcgttcgcc cttcgggccg ttgctaaagc aacgttatcc ttcctggtgc ctgcggttaa
    21001 ttccgctacc tcttcgatat cttcattgct gaagatttcg gagaatatgg tgcatccggg
    21061 aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg
    21121 atgcatcggg aaaatttatt ttactgctga tattgatatc gctactaaag ccatatcaaa
    21181 taagaaaatc catcttaacc atcatcactg atgattacgg agaatatggt gcatccggga
    21241 ggattcgaac ctccgaccgc tcggttcgta gccgagtact ctatccagct gagctacgga
    21301 tgcatcggga aatttatttt actgctgata ttgataccgc tactaaagct gtatcaagta
    21361 agagatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc
    21421 tatccagctg agctacggat gcatcgggaa acttacttta ctgcagattt tttgataccg
    21481 ctactaaagc cgtatcaagt aagagatggt gcatccggga ggattcgaac ctccgaccgc
    21541 tcggttcgta gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg
    21601 ggattcgaac cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca
    21661 gcctctcggc cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct
    21721 catcgtcgct gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc
    21781 cacactttta tcgtttgcac acttcacgct caattagtct gtaaaaacgt caaaaagagt
    21841 gttttatcta cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa aatggagact
    21901 taagttgaat gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg
    21961 tgcgtctcac cgataaagat gagacgcgga aagattagta actggactgc tgggattttt
    22021 cagcctggat acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta
    22081 cgccaatacg tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa
    22141 tcatgagggt ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt
    22201 aaaagagtcg ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc
    22261 aatgacaaac acattacatg taagcagtca tggcattaca ttctgttaaa cctaagttta
    22321 gccgatatac acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg
    22381 tgaagcgagt ccacggcatt gcctgacgct tatattattg caatttcgcg ctgacccagc
    22441 ctttcacact ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca
    22501 tgtcaggacg tccaccaccc ttgccgccca cctgctgagc gaccatacca atcagttccc
    22561 ctgctttcac acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt
    22621 cggctaccgt tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca
    22681 tggtacgcaa cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat
    22741 taacatcaat tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa
    22801 gctgttgtag ttctttttct aactgacgcg tacgttccag tactgagcgc actttatccg
    22861 ccagattatt gctatcgcct ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg
    22921 catgaacggt ggtgatagcg ccttctccgg ttaccgcttc gatacgacga acgcctgcag
    22981 cagtacccga ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag
    23041 taccgccaca cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat
    23101 acttctcgcc gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga tccatgatgt
    23161 tggtttcgat cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa
    23221 tctcttccgg tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa
    23281 ccagtgaacc tttctgcgat acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca
    23341 ggtgcgttgc ggagtgattc aaacgaatac gggcgcgacg agcctcatca acatcagcct
    23401 gcacagcgtc gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct
    23461 ggccgtattt ctgcgtatct tccaccacga aggagaagtt agcgcctttc agttcgcctt
    23521 tatcaccaac ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag
    23581 cttcctggcc tgcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt
    23641 tgccgttcag ttccagatgg tcatagcctt taaattcaga tgcactgtca acacggatca
    23701 ttgcgttgta atcggcacca aagccgctgg cttcgcgcgc ccgacgacgc tgctcttcca
    23761 ttgctgcttc aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg
    23821 tcaggtcaac cgggaagcca taggtgtcgt acagacggaa agcagtttca ccatccagcg
    23881 tatcaccaga aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac
    23941 gagcaaactg ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca
    24001 ggtcttcacc cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg
    24061 tttctttcgc gccgagcata ttaccgtgac gcactgcgcg acgaatgata cgacgcagta
    24121 cataaccacg gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac
    24181 gaatgtggtc agcgattacg cgcagcgatt tattgctcag atcggttgcg ccagtgactt
    24241 tcgctaccgc ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt
    24301 gcagcaccgc agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg
    24361 gttccatcgt gccatcggcc tggcggttga actgcatgaa gacgatgttc cagatctcaa
    24421 tgtagcggtc gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt
    24481 gatcgtagaa gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt
    24541 tgtcagatgc gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga
    24601 tccctacttc tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc
    24661 acagacgctc tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact
    24721 gaatggcatc gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg
    24781 tatggtgacg cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca
    24841 cgcagcgttg ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat
    24901 ccttgaactg gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca
    24961 gggagctgct ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac
    25021 ggatctcagc ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca
    25081 ccagctccag cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt
    25141 ttcttgactg ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc
    25201 tggatatctt ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct
    25261 gaaaagacag ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg
    25321 caccagtcga tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc
    25381 tgattcagtt cctggcgaat acgcgcaggt ccataacctt tgcggctacg gctggcgata
    25441 aagcgcgcaa caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg
    25501 cgctcgtaat cttctgccgt agcatcaatc tcttctgggc catttttgcc cataatcggt
    25561 gccgcgagtt tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc
    25621 gcacgatcca acaggcgagc atatgccggg cgacgggacg ttgattctgt catggcatat
    25681 ccttacaact taaaaagcaa aagggccgca gatgcgaccc ttgtgtatca aacaagacga
    25741 ttaaaaatct tcgttagttt ctgctacgcc ttcgctatca tctacagaga aatccggcgt
    25801 tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt tcgcggtttc
    25861 cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct tctcaccttt
    25921 gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca ggtcaaccag
    25981 ttcgccgtag aagttgatac cttcgccgta gaggatctgg aattcagcct gtttaaacgg
    26041 cgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca cgttttcgcc
    26101 ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga atttcagcgc
    26161 gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca tacggatctg
    26221 gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct tacgcatcgc
    26281 ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc cttcgatttc
    26341 cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg cgccagaacg
    26401 cgccagggcg tcacagattt ccagtgcctg ctcgccggtg tccggctggg agcacagcag
    26461 gttgtcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt gttcagcatc
    26521 gataaacgca caggttttac cttcacgctg cgctgcggca atcacctgca acgtcagcgt
    26581 ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg gcagaccacc
    26641 tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca catccatgga
    26701 acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa tctggcccag
    26761 tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc ctgtcatgcc
    26821 gggtaatacc ggatagtcaa tatgttctgt tgaagcaatt atactgtatg ctcatacagt
    26881 atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca gtagcctgac
    26941 gacgcaccgc atcacggtcg ccgctgaagc attcccgcag ggtaatgcct tcaccgcggg
    27001 cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg ccatccggcc
    27061 cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt gcacctatcg
    27121 ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc tcttcgcgta
    27181 cgccgatcat ttgcgctttg gcttcgttac tgtaggtgac aaatccgcgt tcaaaccagg
    27241 cggaactgcc ggcaatatcg gtaatcactt tcgctaccca accgccggta caagactcag
    27301 cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg cttaactgca
    27361 tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg gacaagatag
    27421 cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt cagaaaaaag
    27481 ctgattagcc agagggaagc tcacgccccc ctcttgcaaa tagttactgc acgcgcgcca
    27541 gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtactg tggttataac
    27601 gggtaatggt gtagaagttc ggcagaccgt accagtactg gtaacctgtt ccaacatcca
    27661 gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt aaacctgccg
    27721 cggcaagctg cgaaatgctg tacctggttt tgaagccatt tggcaagcct ggagcctgac
    27781 cgtttgccat taccgcgacc tgatcgcctt tcacccagcc gtgcgctttg aaataattcg
    27841 ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg ccgctgaaat
    27901 ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc gccccggcaa
    27961 aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc aggaaggttt
    28021 ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc gttgccagcg
    28081 catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg ccgataatcc
    28141 cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac gcatcttcat
    28201 actgattcca gaaaaccaca ccgttctgca cattgtccgg cgtaataaat tttttgcgat
    28261 aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt ggtgcctggt
    28321 tatccatcag ccgcagtacc gaatccagac gtttcgcctg ggagagaatt tcctgcaact
    28381 gctgacgatc gaaaccgtgt ttgttcacca ttctgtcgat gaactgctgg gcattcgggt
    28441 tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc aggaagccgc
    28501 cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg ctacaggcgg
    28561 caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg aggggtccat
    28621 ttaacagatt caaccagggg caagtatggt aaagcatcac gccccgcaca aggaagcggt
    28681 agtcactgcc cgatacggac tttacataac tcaactcatt ctcctcgcta tccttttact
    28741 caaactttca aattaaaata tttatctttc attttgcgat caaaataaca ctttaaatct
    28801 ttcaatctga ttagattagg ttgccgtttg gtaataaaac aataaatcct gaaggagaga
    28861 acaatgatag aaaccattac tcatggtgca gagtggttta tcgggctgtt ccaaaagggc
    28921 ggagaggtgt ttaccgggat ggtgaccggc attcttccgc tgttgattag cctgctggtt
    28981 atcatgaacg cactgattaa ttttatcggt cagcatcgta ttgaacgttt tgctcaacgt
    29041 tgcgccggta accctgtttc ccgttaccta ctgttaccgt gcattggcac gtttgtcttt
    29101 tgcaatccga tgaccttaag cctgggtcgc tttatgccgg aaaagtacaa acccagctac
    29161 tacgcggcgg cctcttatag ctgccactca atgaatggcc tcttccccca tatcaaccct
    29221 ggcgaactgt ttgttatctt ggcattgcca gcggtctgac aacgctgaac ctgccacttg
    29281 gcccactggc ggtgagctat ctgctggttg gtctggtcac caacttcttc cgcggctggg
    29341 tgaccgatct gaccaccgct atttttgaga aaaagatggg cattcaactt gaacaaaaag
    29401 ttcatctggc aggagcaaca tcatgacgca tattcggatc gaaaaaggaa cggggggctg
    29461 gggcggcccg cttgagctgg aaaccacgcc gggcaaaaaa atcgtctata tcaccccgcc
    29521 ggtacccggc ctgcgattgt cgacaaactg gcacagctta ctggctggca ggctattgac
    29581 ggatttaaag aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat cgactgtggc
    29641 ggcacattac gctgcggcat ttatccgaaa cggcgtattc ccaccattaa tatccactcg
    29701 acgggcaagt ccggtccact ggcgcagtac attgtggaag atatttatgt ctctggcgta
    29761 aaagaagaaa acatcactgt agtgggtgat gcgacaccac aaccctcttc cgtgggccgt
    29821 gactatgaca ccagcaagaa aatcaccgaa caaagcgatg gtttactggc gaaggtggga
    29881 atgggtatgg gttctgccgt tgccgtgttg tttcaatctg gtcgtgacac catcgacact
    29941 gtattaaaaa ccattcttcc gtttatggca ttcgtctcgg cgctcattgg catcattatg
    30001 gcttctggcc ttggtgactg gattgcccac ggccttgctc cgctggcgag ccatccactg
    30061 ggtctggtca tgctggcgct catctgctcc ttcccgctgc tttcaccttt cctcggccca
    30121 ggcgcagtta tcgcacaggt tatcggcgta ttgattggcg tgcagattgg tctcggcaat
    30181 attccgccgc atctggcttt acctgcactg tttgccatca acgcgcaggc ggcctgcgac
    30241 ttcattccgg tcggtttgtc gctggcggaa gcccgtcagg acacggttcg cgtcggtgtc
    30301 ccttctgtac tggtgagccg ctttttaacc ggcgcgccga ctgtactgat cgcctggttt
    30361 gtctccggtt ttatctatca atagaggctg aaacatgacc gttatttatc agaccaccat
    30421 cacccgtatc ggcgcgagtg ccactgacgc cctcagcgac cagatgctca tcacctttcg
    30481 tgaaggcgcg ccagcggacc tcgaagagta ttgcttcatt cattgccacg gcgagttgaa
    30541 aggtgcactc catcccggtt tgcaatttac gctcgggcag catcgctatc cggtgaccgc
    30601 tgttggcagc gtggcggaag acaaccttcg cgaactgggt catgtcaccc tgcgcttcga
    30661 tggtttaaac gaagcggaat ttccgggcac tgtccatgtg gcaggccctg tacccgacga
    30721 tatcgcgccg ggatcggttt tgaagtttga atctgttaag gagtaaaaaa tgaatcaggt
    30781 tgccgttgtc atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg gtctggctgc
    30841 cgaggggtat cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa atgtggcaca
    30901 agaaattaac gccgaatatg gtgaaggaac agcgtatggt tttggtgctg acgccactag
    30961 cgagcaaagc gttctggcgc tctctcgtgg ggtagatgaa atctttgttc gtgtggattt
    31021 actggtctac agcgccggaa ttgcaaaagc agcctttatc agcgacttcc agctcggcga
    31081 ttttgaccgt tcgctacagg tgaatctggt gggttatttc ctgtgtgcgc gtgaattttc
    31141 gcgtttgatg atccgcgatg ggattcaggg acgcattatt cagatcaact cgaaatccgg
    31201 caaagtgggc agcaaacaca attctggtta tagcgccgcg aaatttggtg gcgtagggct
    31261 gactcaatca ctggcgctgg atctggcgga gtacggcatt acggtgcatt cactgatgct
    31321 cggtaacctg ctgaaatcgc cgatgttcca gtcgctgttg ccacaatatg ccaccaagct
    31381 gggtatcaaa ccggatcaag tcgagcagta ttacatcgac aaagtaccgc tcaaacgcgg
    31441 ctgcgattat caggatgtgc tgaatatgct gctgttctac gccagtccta aggcgtcgta
    31501 ctgtaccgga cagtcgatca atgtcaccgg cggtcaggtg atgttctgat caacagcgga
    31561 gatccgttaa ggatctccgt gagactaata gaatgcctga tgcgctacgc tcatcaggca
    31621 tacaggactt ccgccactac attaaggaaa agttatggta tccgcactca tcactgtcgc
    31681 cgttatcgcc tggtgtgcgc aactggcctt aggcggctgg caaatttctc gttttaaccg
    31741 tgccttcgac acactatgcc agcaagggcg ggttggcgtg ggccgttcca gcgggcgctt
    31801 taaaccgcgg gtcgtggtcg ccatcgccct ggacgatcag cagcgcatcg ccgacacctt
    31861 gtttatgaaa ggactgaccg tcttcgcccg accgcaaaaa attcccgcaa ttaccggtat
    31921 gcatgcgggt gatttacagc ccgatgtgat ctttccccat gatccactat cacagaatgc
    31981 tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt tgtgaatgtt acttgcttgc
    32041 gaagttatca ttttgaaacc taaatcaggt aatcacgccc atgaaacctc gtcagcgtca
    32101 agccgccatt ctggagtatc tgcaaaagca gggaaaatgc tcggttgaag aattggcgca
    32161 atattttgac accacaggca caactattcg caaagatctg gtcattctgg aacatgccgg
    32221 aaccgtcatt cgtacttatg gcggagtggt gttgaataaa gaggaatccg atccgcctat
    32281 cgatcataaa acactcatca acacccacaa gaaagagctg atagcagaag ctgccgttgg
    32341 ttttattcat gatggcgatt cgatcattct tgatgctggc agtaccgttt tgcagatggt
    32401 gcctctgctc tcgcgcttta ataacatcac ggtgatgacc aacagcctgc atatcgtcaa
    32461 tgcgttatct gaactggata atgaacaaac tatcctgatg ccaggcggaa cgtttcgtaa
    32521 aaaatcggcc tcatttcacg ggcagctggc agagaatgcc ttcgagcatt tcaccttcga
    32581 caaattattc atgggtaccg acggcatcga tctcaatgcg ggcgtaacca ccttcaacga
    32641 ggtctatacc gtcagtaagg caatgtgcaa tgccgcgcgc gaagtgattt tgatggcgga
    32701 ctcatcaaag tttggccgta aaagccccaa cgtagtttgc agtcttgaaa gcgtcgataa
    32761 gctgattacc gacgcagata tcgatccggc gtttcgtcag gcgctggaag agaaagggat
    32821 cgatgtgatc ataaccggag agagcaatga gtgaagcact actgaacgcg ggacgtcaga
    32881 cgttaatgct ggaattgcag gaagcaagcc gtttaccgga acgtctgggc gatgattttg
    32941 ttcgcgccgc caatatcatc ctgcactgcg aaggcaaagt ggtggtttcg ggaattggca
    33001 aatcgggcca cattggtaag aaaatcgccg caacgcttgc cagtaccggc acaccggctt
    33061 tttttgtcca tccggcagaa gcgctgcatg gcgatctggg gatgatcgaa agccgcgatg
    33121 tgatgctgtt tatctcttac tccggtgggg cgaaggaact ggatctgatt attccgcgtc
    33181 tggaagacaa atctatcgcg ctgctggcga tgaccggcaa accgacgtca ccgctgggcc
    33241 tggcggcgaa agcggtgctg gatatctccg tagaacgcga agcctgcccg atgcaccttg
    33301 cgccgacctc cagcaccgtc aataccctga tgatgggtga cgcgctggcg atggcggtca
    33361 tgcaggcgcg cggatttaat gaagaagatt ttgcccgctc ccaccctgct ggagcactgg
    33421 gcgctcgctt gctgaataaa gtgcatcatc tgatgcgccg tgacgatgcc atcccacagg
    33481 tggcgttaac cgccagcgtg atggatgcta tgctggaact cagccgcacc ggtctggggc
    33541 tggtggcggt atgtgacgct caacaacagg tacaaggcgt ctttaccgac ggcgatttac
    33601 gtcgctggct ggttggcggc ggcgcactca ccacgccagt caatgaagcg atgacggtcg
    33661 gcggcaccac gttgcaatcg caaagtcgcg ccatcgacgc caaagagatc ctgatgaagc
    33721 gcaaaatcac tgccgcaccg gtggtggatg aaaacggcaa actcaccggc gcaataaacc
    33781 tgcaggattt ctatcaggcc gggattattt aatccttcaa tcccagacgt ttcgccagcc
    33841 gatgcaggtt ggcgacgtcg gtttccagca tccgcgcgca ggcagcccag ttgtgatgat
    33901 tttgtgccag tgcctgacga atagtttcac gctggaacgc ttctgtcgct tcacgcaggt
    33961 tttgcttaac aacgggcacc gccgccactt ctggcggcgg caacgtcacc tcaggaaaag
    34021 caaaatgttg cgcctcaaga atcacttcat cgccgctgcg ggtggctctc gccagcacta
    34081 ccgcccgatg aatagcatgt tccagttcgc gcacgtttcc cggaaaactg tagtgttgca
    34141 gtaaatttcg cgccccggca cttaatacca cgcgggagag cccctgccgc aaacgacact
    34201 gctcgcagaa ataccccgcc agcagaatga catcatcgcc ccgctcacgc agcggcggca
    34261 ccgaaagtgg aaacacactc aggcgatgaa acaaatcggc gcggaatcgc cctgccagca
    34321 cctcttcgcg taagtcgcgg ttagtcgccg ccagcacgcg cacatcgacc cgcaaactac
    34381 ggtcatcgcc aacgcgctga atatcgccat actgcaacac cctcagcagc ttggcctgca
    34441 atgccaacga caactcgccg atctcatcga gaaacagcgt gccgttatcc gccatttcaa
    34501 acttcccact gcggttactg atagcgccag taaacgctcc tttcacatgc ccgaacaact
    34561 cactttccgc cacactttcc ggcagtgcgg cacagttgag atagaccagc ggattcaccg
    34621 cccgtggcga ggcctcatga atcgctttcg ccaccagctc cttaccggtt cccgtctcac
    34681 cgctgatcag gacgttgaga tcggacgccg ccacaatctc aatctctttt ttcagttgcg
    34741 tcatgcctgg cgacagaccg atcatctgcg tctgtttcac cgcttcaaat ggagcggcat
    34801 cgcccggcag catattctgg ctttccagtt gttcaatcag caacgcattg cttaacgctc
    34861 ccgccgccag cgcggcaatc aggcgtaact cttcgtcgct gaaaacatcg aactgatcgg
    34921 gctgcatccc gtcgagcgtc aatgcgccga tcaggttttg cccggcaaac agcggcagac
    34981 caacgcaggc gtgaaccttc agactctcct gccctggaat caaaccgtca tagggatcgg
    35041 gcaattcgct gtctgcggga aagcgcacca catcacccgc gcgggcaatc gcttccagcc
    35101 gtggatgacc ttccagcgca aagcgtctac cgagtacatc cttcgccagc ccgtcgatgg
    35161 caagcggaat aaactgccgc gagtcgtaac gtagcaacgc agacgcatcg cactccagca
    35221 cctgacgcag cgtggtgatc aggcgctgaa aacgatcctg atgaccaatc ccacgctgca
    35281 attcgatggc gatattcgcc agcacatcaa cggaaaaact catctttgcc tcactgtcaa
    35341 tttgactata gatattgtca tatcgaccat tggattgata gtcattttga ctactcatta
    35401 atgagcataa ttttatttat agagtaaaaa caatcagata aaaaactggc acgcaatctg
    35461 caattagcaa gacatctttt tagaacacgc tgaataaatt gaggttgcta tgtctattgt
    35521 ggtgaaaaat aacattcatt gggttggtca acgtgactgg gaagtacgag attttcacgg
    35581 cacggaatat aaaacgctgc gcggcagcag ctacaacagc tacctcatcc gcgaagaaaa
    35641 aaacgtgctg atcgacaccg tcgaccataa attcagccgc gaatttgtgc agaacctgcg
    35701 taatgaaatc gatctggcgg atatcgatta catcgtgatt aaccatgcag aagaggacca
    35761 cgccggggcg ctgaccgaac tgatggcaca aattcccgat acgccgatct actgtacagc
    35821 caacgctatc gactcgataa atggtcatca ccatcatccg gagtggaatt ttaatgtggt
    35881 gaaaactggc gacacgctgg atatcggcaa cggcaaacag ctcatttttg tcgaaacacc
    35941 aatgctgcac tggccggaca gcatgatgac ttacctgaca ggcgacgcgg tgctgttcag
    36001 taacgatgct ttcggtcagc actactgcga cgagcatctg ttcaacgatg aagtggatca
    36061 gacggagctt tttgagcagt gccagcgtta ctacgccaat atccttacgc cgttcagccg
    36121 tctggtaacg ccgaaaatta ccgagatcct gggctttaac ttaccagtcg atatgatagc
    36181 gacctctcac ggcgtggtat ggcgcgataa cccgacgcaa attgtcgagc tgtacctgaa
    36241 atgggcggct gattatcagg aagacagaat caccattttc tacgacacca tgtcgaataa
    36301 cacccgcatg atggctgacg ctatcgccca ggggattgcg gaaaccgacc cacgcgtggc
    36361 ggtggatcgt ctttccacgc gcctgcagga tgcgggtttc gaaatgtcgc ttagcctgaa
    36421 agcgaaatgg cgaccagacc aggacgctct ggagttatgc cgtgaacacg gtcgcgaaat
    36481 cgcccgtcag tgggcgctcg cgccgctgcc gcagagcacg gtgaatacgg tagttaaaga
    36541 agaaacctct gccaccacga cggctgacct cggcccacgg atgcagtgca gcgtctgcca
    36601 gtggatttac gatccggcaa aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg
    36661 gagtgaagtc ccggataact tcctgtgccc ggaatgctcc ctcggcaaag acgtctttga
    36721 cgaactggca tcggaggcaa aatgagcaac ggcattgtga tcattggttc gggcttcgcc
    36781 gcccgccaac tggtgaaaaa tattcgcaaa caggacgcca gtattccatt aaccctgatt
    36841 gccgccgaca gtatggatga gtacaacaaa cctgacctca gccatgttat cagtcagggg
    36901 caacgtgccg acgaccttac ccgccagacg gcgggtgaat ttgccgagca gtttaatctg
    36961 ctcctgtttc cgcaaacctg ggtgacggat atcgatgccg aagcccgtgt ggtgaaaagc
    37021 cagaataatc agtggcaata cgacaagctg gtgctggcaa ccggtgccag cgcctttgtc
    37081 ccgccagtgc ctgggcgtga gttaatgctg acgttaaata gtcagcaaga gtatcgcgcc
    37141 tgtgaaacgc aactgcggga tgcccgacgc gtgttgattg ttggcggtgg tttgattggt
    37201 agcgaactgg cgatggattt ttgtcgtgca ggcaaaatgg ttacgctaat cgacaacgct
    37261 gccagtattc tggcgtcgtt aatgccaccg gaagtaagca gtcgcttgca gcatcggttg
    37321 acggagatgg gcgttcatct gctgttgaaa tctcagttgc aggggctgga aaaaacggat
    37381 tctggcattc tggcaacgct ggaccaccag cgcagcatcg aagtggatgc ggtaattgcc
    37441 gccaccggac tgcgcccgga aaccgccctg gcacgacgcg ccgggctgac gattaatcgc
    37501 ggcgtttgcg tcgatagtta tctgcaaacc agtaatgccg atatttacgc gctgggcgat
    37561 tgcgcggaaa ttaacggtca ggtattgccg ttcctgcagc cgattcaact tagcgcgatg
    37621 gtgctggcaa aaaatcttct cggcaataac acgccgctga aactaccggc gatgctggtg
    37681 aaaatcaaaa cgccggaatt accgctgcat ctggcaggcg aaacccagcg tcaggattta
    37741 cgctggcaga ttaataccga acgccaggga atggtggcgc gcggtgttga cgatgcagac
    37801 caacttcgcg cctttgtggt cagtgaggat cggatgaaag aggcatttgg attgttgaaa
    37861 acgttgccgg tgtaggtggg ctactgtgcc taaaatgtcg gatgcgacgc tggcgcgtct
    37921 tatccgacct acggggacgc atgtgtaggc cggataaggc gtttacgccg catccggcaa
    37981 tggtgtccaa atgcaacacg ttttatccgt tctggacttc acccgctaac caacgcgccg
    38041 cagcaataac cccctgcccc agagacaaac cgccatcacc cgccggtaaa ctctgtggaa
    38101 acagcaatgt gaaatcagcg agataatgcg ccagacgtgc acgcagcaaa cggttatgaa
    38161 taaccccgcc gctaaatacc agcgtagtga taccacgcat cgtggcctgc tcacgcatca
    38221 acgcggcaaa accctgcgcc agcgcatcat gaaacgccca cgcgcgttga ttaaccggtg
    38281 cctgccagtt cagccactgc tgccagaaag tggcgagatc cagttgattg tccacccgcg
    38341 gcatcgtcac cggatgcgtc actccgtcgc atgaggctgc tagcgcctcc agagcacaag
    38401 ccgcttcacc ttcataactt aacgtggctg gcgcacagcc cagtgccgcc gccacagcat
    38461 cgaacaaacg cccacacgat gacgccagcg gcgcgttaat tccacgctca atggcccgcg
    38521 ccagtacgct ccagttttgc tgttgcacac ttgccgtttc agggtaattc tgccactccg
    38581 gcacaaagcg caggcactgc gccagcaggt tacgccacgg ctgcttcgcc gccaaatcgc
    38641 cacccggaag cgccactgca ggcaagccgc ccaggtgctc acattcgcga tagttcaccc
    38701 gcaggcactc gccgccccac aaagcgccgt tctcccccat accgataccg tcgagcgtca
    38761 aagcaatgac atcaccgcca tccagcggcc actgatgctc tgccagacac gccgctgcgt
    38821 gggcatgatg atgcagcacc gtttgcgtcg gcagattcat ttcgctcgcc cactggcagg
    38881 agacatagcc cggatgtgcg tcatgcacaa cgtattgcgg agtgaaattg tagatgtttt
    38941 gcatcaggcg taacgcttcg cgccactgcg tctggatgcc atcgtcactt aaatcgccca
    39001 gatgctggct caacaccact tgttcaccgc gcaccaggca gaaggtattt ttcagatccg
    39061 cgccgagaca cagtacaggc ggaacatttt taaagcccgg aggcaaagcc agcgcatccg
    39121 gcacataccc ccgcgaacgg cgcagcattt cgccgctttc gcgcaccacc gaatcatcca
    39181 tccgctgcac aatgtcgcgg ttgtgtatta agaatccgtc ggcaatgccc tgcaaatcct
    39241 ccagcgcctg ttcgttgctg atagccggtg gtttaccgct caggttgcca gaggtcatca
    39301 ccagcgggca ttgtagttcc tgtaacagca aatgctgaag cggattcgca ggcaacatta
    39361 ccccgacttc gttaaggcca ggggcgatat catcacaaag ctcaggaacg tattttttat
    39421 ccaccagcac aatcggcgcg gcgggcgtgg taagcaactg gcgcgcagcg tctggtaaac
    39481 cgtcagccac tggcaacatg actgccagcg gtttcgccgg gcgatgtttg cgcgcccgaa
    39541 gtgtcgccac cgcgttactg ttacgtgcat cgcaggcaag atgaaatccg ccaatccctt
    39601 tgatggcgac aatgttgccc atttttaact gtgcgatagc cgcctgtaat gccgcctctt
    39661 gttctgcatg ttcaccatga cttacccatt caagatacgg gccacactcc gggcaggcca
    39721 ccggctgggc gtggaagcga cgatcgagcg ggtcgcggta ctctttatca caggctggac
    39781 acagcggaaa cgccgccatc acggtaaacg ggcgatcgta aggcatggcg cgaataatgg
    39841 tgaagcgcgg gccgcagtgg gtacagttga taaatggata acgataacgc cgttcgcctg
    39901 gggtattcat ttcggcaagg caagcagggc aagtggcggc atcgggaaca atttgcgtat
    39961 tcatggcgcc gcccgcgctc tggcggatgg tgaactcagt gggcagttgt gaccagataa
    40021 acggttcacg ctcaacgcta tcaatacgcg ccagcggcgg gcagtgctga tgcaattgaa
    40081 caagaaacgt ttccgggtct tccagcagcc ggacttctac gccatcgccg tcattacaga
    40141 catcgccgtg aagatttaat tgctgtgcca gctgccagac aaacggacga aaaccgacgc
    40201 cctgcacttt gcctcgaata cgcagttgga caccgcaaga tgtgtttttt gccattgagt
    40261 tattcccgcc atcatgaatt gcgtaacccg tccagccgga cacgacagcg tcgcatccgg
    40321 cagtcacagg tcggcgatac cgcccgctcc gtattctacg aatatttccg ggaattcctt
    40381 tgatgccaga acagttctgt aagattttta gaacatcagc gccgtacggc ggcgtttttc
    40441 tgcgctcagt tgttcaagtt tattgcgatc gacacaaatt agcgcatggg tcgggcaagc
    40501 cgccatacac gccgggccgt cttcacgatg gttgcacagg tcgcatttat tggcttcggc
    40561 tttgtcagcc cgtacattca gccctgcgcc gctgttgcga atcaccggac gtaccaccac
    40621 ttccatcgcg ccatacgggc aagccacaac gcaggttttg caaccaatgc aacgttcctg
    40681 catcacatga acaaaccctt tatcacggct gatagcacca ttcgggcaga cgttagcgca
    40741 cggtgcatct tcacactgac ggcaaactgt cgccgtggaa atgttcacac ctttaatgac
    40801 atggatacgc ggtaaaaatg tttccggggt cagcgatgca cagtcctgat tttcctgatg
    40861 agaaaccacg cacgccactt cacaggtacg gcaaccaata catttactcg cgtcagcaat
    40921 gatgaaacgg ttcatcaaat tctccagcaa tgacagttaa tgcgccgata cattcacaaa
    40981 tcatgccagt ttttaatttg ctgttattta aggaaattaa tttctgtaat gcaggaaaaa
    41041 agatgtcatc gacactagtg acgatgacat gtgatgacaa tgtttatcgc gaaagagcaa
    41101 tgagtgagtc gcggcggatc agttttccgc tgaaggtttt cggcggtgag aaatccccgc
    41161 catcgagcat aaaaatcagc cgtccaataa tttcctgaat catctcagtc accggaattt
    41221 ttacgctgga gagcgccgga acgatatagg gggcaattgc gatatcatcg aatccgataa
    41281 ctgacacctg ctctggcacc gctacgccgc gcttgtgtaa cgctttcatc gcacctatcg
    41341 ccatatcgtc gttactggca actaacgcgc taaatttagc cccacgttcg agcaacgttt
    41401 ctaccccttc ggccccgctg gcgggcgtcc atttaccgtt agcgataagt ttttcattga
    41461 gcgcaatacc atgctgcgcc agcgcgtctt tatatccggc aagacgttca acgctggtgg
    41521 gggaatccat cgagccggta agaaaagcaa tctcctgatg cccggcgttt atcaactctg
    41581 ccacggcgtt aaagctggtc tgtttatgat cgcaccagac gctatggctg ctatttttgc
    41641 gcaggcggcg attaagcacc attatcggct gactgtgcgc gtcaatgatg tcatcgatct
    41701 catccacact taaaaaacgc gggtaaatca taatggcgtc gcagcgcaaa tccagcagat
    41761 actgaatcgc ctggcgctct tcttcggcgc tgtgtttacc atctgccaat agcaactgcc
    41821 gccctttctc ttccgccatt cgcgcggcat gaaacagtaa ttcactaaaa taaatgccgt
    41881 ggtaaagcgt gttagtcact accagcccca gcgtctgggt acttttcgcc gacagattgc
    41941 gcgccagcaa gtttggacgg taaccgctct cttctaccgc ctgaaacacg cgatctttag
    42001 tctcctggct gacgtagcca ttacctgaaa gcacgcggga aacggtcgct tttgaaaccc
    42061 cggcgcgctt cgccacttcc agcatcgtcg tcatcatttt catcccttta cacgcaatca
    42121 acgcagtgta ctgcaccgtt taccgattgt ccttgcgcaa tcggcgggaa aaatattcag
    42181 gtgaccggtt tcacaaatat aaaaaatgaa caattaactc tcttgcttat ttggttacaa
    42241 ctattcatga ttttatgaaa ccggtttctt aattccgttt cagcatcggc atttttccgt
    42301 cacgtcgact gataacaact acatcttccc tactgataac aggataaaat ccgatggcca
    42361 aaaattatgc ggcgctggca cgctcggtga tagcggcact gggcggcgtt gataacatct
    42421 cggcggtcac gcactgtatg acgcgtttgc gctttgttat caaagatgat gcgcttatcg
    42481 acagcccgac gttaaaaacc atccccggcg tgctcggcgt ggtacgtagt gacaaccagt
    42541 gtcaggtgat tattggcaat accgtttcac aagcctttca ggaagtcgtc agcctgctgc
    42601 cgggagatat gcagcccgcc ctgcccgtgg gtaaacccaa actcacgctg cgtcgcattg
    42661 gtgcggggat cctcgatgcg ctgatcggca ccatgtcacc gctgatcccg gcgattatcg
    42721 gcggatcgat ggtcaaactg ctggcgatga tcctcgagat gagcggcgtg ctgacaaaag
    42781 gatcgccgac cttaaccatt ctgaatgtga ttggtgacgg tgctttcttc ttcctgccgc
    42841 tgatggtcgc ggcttcggcc gccatcaaat ttaaaaccaa tatgtcgctg gcgattgcca
    42901 tcgcgggtgt gctggtacat ccgagtttta ttgaactgat ggcgaaagcg gcccagggtg
    42961 aacatgtcga atttgccctg attccagtca ccgcggtgaa atacacctac acagtgatcc
    43021 cggcgctggt gatgacctgg tgcctgtcat atatcgaacg ctgggtggat cgcattacac
    43081 cggcggtgac caaaaacttc ctcaagccaa tgctgattgt gttgattgcc gcaccgctgg
    43141 caatcctgct gattggcccg attggtatct ggatcggtag cgccatttcg gcgctggttt
    43201 acaccattca tggttatctg ggctggcttt cagtcgccat tatgggcgcg ctgtggcctc
    43261 tgctggtaat gaccgggatg caccgcgtct ttacgctaac catcattcag accattgccg
    43321 aaaccggcaa agaagggatg gtcatgccgt cagagatcgg cgctaacctg tcgctgggcg
    43381 gttcatcact ggcggtggcg tggaaaacga aaaacccgga actgcgccag actgcgctgg
    43441 ctgcggcggc atcagccatt atggcgggga tttccgaacc ggcgttatac ggcgtggcga
    43501 tccgcctgaa acgtccgctt atcgccagtc ttatcagcgg ttttatttgc ggcgcggttg
    43561 ccggtatggc ggggcttgcc agccactcaa tggcagcgcc ggggctattt accagcgtgc
    43621 agttcttcga tccggcgaat ccaatgagca tcgtctgggt gttcgcggtc atggcgctgg
    43681 cggtggtgct gtcgtttatc ctcacactgt tgctcggctt tgaggatatt cctgttgagg
    43741 aagcggctgc cgaggcgcga aagcatcaga gcgcacaacc gaccgtcgcc aaagaagtaa
    43801 gtcttaattg aggatgaaaa tgtcagtatt tccagaaggt tttttatggg gcggcgcgct
    43861 tgccgccaac cagtctgaag gtgcgttccg tgaaggtggc aaaggactga caacggtcga
    43921 tatgatccca cacggcgagc atcgaatggc ggtgaaactg gggctggaaa aacgttttca
    43981 gttgcgcgat gacgagtttt atcccagcca tgaggcgacg ggtttttatc atcgttataa
    44041 agaagatatc gccctgatgg cagagatggg attcaaagtg ttccgtacct caattgcctg
    44101 gagccgcccc ttcccgcagg gtgatgaact gacgcccaac cagcagggca ttgcttttta
    44161 ccgttctgtc tttgaagagt gtaaaaagta cggtatcgaa ccgctggtca cattgtgcca
    44221 cttcgatgtg ccgatgcagc tggtcaccga atatggctcc tggcgtaacc gcaagctggt
    44281 ggagtttttc agccgctacg ccaggacctg ctttgaagca tttgatggtc tggtgaaata
    44341 ctggctaacc ttcaatgaaa tcaacattat gttgcatagc ccgttctccg gcgcggggct
    44401 ggtgtttgaa gaaggtgaaa atcaggatca ggtgaaatat caggccgcgc atcaccagct
    44461 ggttgccagt gcgctagcca ccaaaatcgc ccatgaggtt aacccgcaaa atcaggtggg
    44521 ctgtatgctg gcgggcggta acttctaccc ttacagttgc aagccggaag atgtctgggc
    44581 ggcgctggag aaagatcggg aaaacctgtt ttttatcgat gtgcaggcgc ggggcgcgta
    44641 tccggcttac tctgctcgcg tattccgcga aaaaggggta accatcgaca aagcaccggg
    44701 cgatgatgag atcctgaaaa acaccgtcga ttttgtctct ttcagctatt acgcctcgcg
    44761 ctgcgcctcg gcggagatga acaccaacaa tagcagtgcg gcgaacgtag tgaaatcgct
    44821 gcgtaacccg tatctacagg tgagcgactg gggctgggga attgatccac tcggtctgcg
    44881 tatcaccatg aacatgatgt acgaccgcta tcagaagccg ctgtttctgg tggaaaacgg
    44941 cctcggcgca aaagatgaac ttgctgccaa tggcgagatt aacgatgact atcgcatcag
    45001 ctatttacgc gaacatatcc gcgcaatggg cgaagcgatt gcagatggca ttccgctgat
    45061 gggctacacc acatggggct gtattgattt agtttccgcc tctacgggtg aaatgagcaa
    45121 acgctacggt tttgtctttg ttgaccgtga cgacgcaggc aacggcacgc tgacgcgcac
    45181 acgtaagaaa tcgttctggt ggtataaaaa agtgattgcc agtaatgggg aagatttaga
    45241 gtaggtaacc tactgtcgca ctaaccagga aggcccgata agcgttgcgc atcgggcaat
    45301 ctggggtttg ctatcagttc tgattccctc atcaagaaca tccctgtcct gattacttat
    45361 tgaaaaacta ctcttcttcc accgctaact gcgcgaagcc gccgttccct tcccagcctt
    45421 ccagtcgttg ataaacggtt tctaccgcat ctttaatcgg ctgggtcatc gggtagtaaa
    45481 agccgacgat atccggctgg atgccgagga aaatcacttc gccaatatct tctttcaact
    45541 gatcgataag gtagttcaac ggcatgttat gggtagtcat cataaacatc tcggcgatat
    45601 catccgggtc gatgatgcgg atttcgccgg ggtttagccc catatccgtg gcgtcgacaa
    45661 tcagcagccg tgtcgggcgc agttcgcgga tagcgacaat gtcgttttcc ggtgcgctac
    45721 cgccgtcaat caccacccag ttacctttcg gcgcggcggc gcacttttcc gccagcagcg
    45781 gacctgcgcc atcatcaccc atcatgctat tgccaacaca gagtaaaacg tcagtcacgc
    45841 agtctcctca ccatcaaata gatggcgttt tcctgatgaa tatcatgcag catacttaac
    45901 agcattgtac tccattcctg ctgctgcgaa gtttgcgctg cccgggcttt atcgaatgca
    45961 ttggccagca tcggcacatg gttaatgtcg atgacgatct caccatattt cggcacacct
    46021 tccattttgc ggcgggcttc gctgcccgcc tccagagtgg caatccatgc cagatattca
    46081 tcccacgggc aggtgagcgc cgcttccagg caatcgataa ccccaaggtg gtgaccaatc
    46141 gccaggctgt aatagaccac ctgctgcgcc tcggcgggcg tggcatcgtt ctcatcaata
    46201 aatttacggc tcagttgact gaacaccacc ttttcactca tcggatacgc gcctcttcaa
    46261 caacatgatt cagatggctg acaatctcgt tcagacgcgg atcgttttcc gcttccagcc
    46321 agcgtgcaac ctgttcttcg ccctgcccta actgtgtaag gtaatcatcg gcaatctgac
    46381 gaccgtaacg ataacccgcc agtcgacgtg cttcgcgatc cactttcaca cgcagcggct
    46441 gcaccatatc gccatgcagg atctccgccg gttgttcatc ctgctcaccc ggcccacggg
    46501 cgtgaatttt ctgctccagc aggccgagcg ccattgcaaa gccgtacagc gtggcggctg
    46561 gcgttggcgg gcagccaggg atataaacat ccaccgggac gattttatcc gtaccgcccc
    46621 acacgcagta gagatcgtgg aagatcccgc cgctgttacc gcaggcaccg taggagatac
    46681 aaattttcgg gtccggcgcg gactgccacg cacgcagcgc aggggatcgc attgcacggg
    46741 tgaccgcgcc ggtaaacagt aaaatatccg catgacgcgg tgaaggaacg actttaatgc
    46801 cgaagcgttc tgcatcgaac agcggcgaca aagtggcgaa aatttcgatt tcgcagccgt
    46861 tgcagccgcc gcagtccacg cggtaaacgt aggcagaacg tttgattttt ttcagtaacg
    46921 acgccttcat gctggcgatg gattcatcca ccgtcatcgg gaccggaatg ccgttggcgt
    46981 cacgggggcc taataaattg ctcatcagat ggcctctttc atatggcgag tcagttcaat
    47041 acggtcggac ggcaccaggc atttctggcg cttacattcc gggcaagtct caaagctttc
    47101 gcggtggttt tccgcgcggc tgtcgccatt gtgcttaagc agcgcaatgg cgtagtcgat
    47161 ctctttctgg acggcgaaag gacgattgca gacgcggcag ttgcacagcg cgaagcggga
    47221 ctgttgcagg aagtcttctt tcttccacac cgccagttcg tactcttgcg acagtttgat
    47281 tgccgccgtc gggcagactt cttcgcagcg tccgcagaag atgcagcgcc cgagattgaa
    47341 ctcccacgcc agctcaccgg tggcgaggtc agtttcaacc gttaaggcgt ttgacgggca
    47401 ggcattgacg caggccgcgc agccgatgca ctgctgcggg ttctgctctg gcttaccacg
    47461 gaagttttta tcaaccgcaa tcggttccag cggataagac gaggtcgccg tgccggtttt
    47521 gatgactttt ttgataaagg taaacatggc gattccttat ttcagcggcg agtttttacg
    47581 ctcaatgctg taacgctcga gttctttgta cggcaccact ttgctctttt tcttacgcac
    47641 atcgaccacg gtcatgcggt cggtacagga gtagcaaggg tccagactac cgataatcag
    47701 cggcgcatca gaaacggtgt tgccgcgcag catgtagcgc agggtcggcc agttcgcgta
    47761 ggtcgcggca cggcagcgcc agcggtacag cttctggttg tcgccggtca tgctccagtg
    47821 gatatcatcg ccgcgcggcg cttcggcaaa gcccagcgca aaacggtgcg gaatgtaggt
    47881 aaagccttcc accatcagcg ggccgcccgg caggttatcc agaccgtagt cgatcatgtt
    47941 cagcgcggta tagacttcgt taatacgcac tttcagacgc gaaataacgt cgcagccctg
    48001 ctcgctgtgg acttccattg gcagcaggcc ataaccaaca aacgggtgat cggcgcgggt
    48061 atcgcgggcg tgaccgctgg cacggaccat cgggccgacg ttactgaagt cgcgagcgat
    48121 ttccgggtcc agacgaccaa tgccgacagt gcgctgttcc atgttcggcg tactcagcag
    48181 cacatccacc agctcctgca cttcacgacg catctgttgc gccagttgac gggtctggat
    48241 catgtcatct ttcagcagat cgcgacgaat cccgccgatc aggttcaggc cgtaggtttt
    48301 acgcgcaccg gtaaggatct ctgccatttt catggaggtt tcacgcacgc ggaagaactg
    48361 cataaagccg gagtcaaagc cggtaaagtg acaggcgagg ccgaggttga gcagatgcga
    48421 gtgcagacgt tctacctcca gcagaatggc gcggatcatc tgcgcacgtt ctggcacctg
    48481 aatacccatc gcgttttcca ccgacgtggt gtaggcggtg ctgtgagcaa aaccgcagat
    48541 cccgcacaca cggtcagaga ggaaggtcac ttcgttataa cccatgcggg tttctgccag
    48601 tttttccatg ccgcgatgga catagaacag gcggtagtcg gcgtcgataa tgttttcgcc
    48661 atcgacgaac agacggaagt ggcccggttc gtcagaagtg acgtgcagcg gaccaatcgg
    48721 cacgacgttg tttttcttgt cgcccagttc gttgatgaac tcgtaggttt cagcatcggt
    48781 ggtcggtgcc ggacgctgac gataatccat gctgtcttta cgcagcggat aaagttcatc
    48841 cggccagtca tccggcagca ccagacgacg ttcatccggc aaaccaaccg gaatcaaacc
    48901 gtacatatca cgtacttcac gctcgcccca caccgcagcc ggaacgcgcg gcgtcacgga
    48961 aggatactcc ggtttgttgg catcaacttc gacgcgaacc gttacccagc acttagtccc
    49021 cttctccatc gacagcacgt agtaaacggc gtaatgacca ttcagtttgc gttcgtcgtt
    49081 accaaaaagc accgacagcc agcccccctg cttgtagtag agaaactcca ccacttccgg
    49141 cagatagttc acctttacgg tgacagtcag ctgatctttg gtctgccagg cgtggtccag
    49201 cacgacgccc ggaaatgcct cattcagcgc ggcgagataa tgttgaccta atttttcttc
    49261 agacatgctc aaactctctt taatcacgcc gccagcaagg agacgaacgc taaaaatgca
    49321 aagccaaacc cagcccaggt aatgcgcgga gtaatatcaa gacgcagacg ggccatgctg
    49381 ttttcgaaca gcgcgataac caggacgccg accaccagtt tgacgatggc aatcaccaac
    49441 gccagcagca gcccaccgac ggtgaaggtt tccatttgtc cccacggaat aaacaccccg
    49501 acgaacatct gcagcaccac caactgtttc aggctgatac cccatttcat gacgccaaag
    49561 ccgctgccgc tgtactcaga gagcggacct tcctgcaact cctgctcggc ttccgccagg
    49621 tcgaacggca gtttgcccat ttcgataaaa gtggcgaacg cacaggcaca aagcgccagc
    49681 accaacggga tgctctggct cagcggccag tgataaacgg tgtcggtgat gttgctgatg
    49741 ttggtggaac tggcaacctg tgcggcaacc cacagaccaa gcagcagcat cggttcgacc
    49801 agcacgccaa gcatcgcttc acggctcgcg ccgatagcgg taaacggact accggtatcc
    49861 agaccagaaa tggcaaagaa gaaacgcgcg atggcaaaga gatacagtaa ggtgatcaaa
    49921 tcacccagtt gcggcagcgg agaaccgacg gtcaccaccg gcagcgcagt agcgatagtc
    49981 agcatgacgc ccaccatcac atacggcgtc aggcggaaca cccagccgga ggcatccggg
    50041 ccaacgctct ggcgacccag cagtttgata atgtcgcgat actcctgcaa cacgcccggc
    50101 ccgcgacggt tatgcaagcg ggcgcgcgcc acgcgggtaa taccggagag cagcggcgca
    50161 acggcaaata gcaccagcgc ctgaattaac ggatataaaa cactcattct caggctcctc
    50221 gtgaaacaat aatcaccacc agcaccgcca gttcaaccag cgccatccgg cggaacagca
    50281 acgcactgcc ttcgcactgc cagcccggca ccagcgacac cggattcagc catttgcgca
    50341 gtttcagcac cggcgcaaac gcctgtttca ccggcatggc aaaaccgtgg gcggtaatta
    50401 ccattgattt ttcatggtcg tagccgcaca cccaggccgc gccgcgggaa cgcgatggca
    50461 aacgatcgcc tttgcaaatc gccataatga tgaatggcag cagcgggcag gcaatcagca
    50521 gcaacgtgat catcggttga gaaacggtgg tgttagcagg ttccagcggc agaggtacag
    50581 cagcagagag catcggcagc agccacggcg cagcaacacc gccaattacg cagcaaatcg
    50641 ccagtgccac tacgcttacg ctcatcagga gcggtgcaca ggtggcgttt tcggcttctt
    50701 tggtacgcgg cgcgccgagg aacgtgacgc catagacttt cgccatacac atcaccgcca
    50761 gcgcaccggt aattgccaga cccacagcga gcagcggccc cagcagacgg gcaacaaacg
    50821 cgccactatt gctcagtttg aagaaggatt gatagataac ccattccccg gcaaaaccgt
    50881 tcagcggcgg cagtgcagcc attgccatca gcccgactaa catagcgatg gagataaccg
    50941 gcattttctt gccaatacca ccgagttttt cgatatcgcg atgaccggta cggaaccaga
    51001 cgctccccgc cccgaggaac agtacgcttt tgaacaggct atggttaagc agatggtaca
    51061 gaccaccgac caggccaaga gcaatcagcg ccggttgttc gagcgcgata cccgttacgc
    51121 cagcgcccag ccccagcagg ataatgccga tattttccag ggtgtggtaa gccagtagac
    51181 gctggatatt atgttccatc aacgcataca gaccgccgac aaacgcggtg atcataccga
    51241 gcaccagcag cgcgatcccc caccacagcg gtgcattacc gcccagcagt gacagggtta
    51301 aaatgcccag caggccaatt ttcatgacta ccgtagaaaa caacgcggca gctggcgcag
    51361 aggcgttcgc atgtgcctgc ggcacccagc cgtgcagcgg aataatcccg gccagcaggc
    51421 caaagccaat cacactgagc agccagatat cggaaccgag cggcagctgt tgcatacgca
    51481 tatccagcag gcgcagatcc agcgtgccgt aacgctgcca cagcagccag caagcaatcg
    51541 ccagcagcag agtgccaaga cgccccagcg caaaccacag tttgccctct ttgctgttgc
    51601 tggtgaggaa caccgcacac aaggccatga cttcggccat taccacgaac atgccgaggt
    51661 tgctggcaat gacggcgcag acggcggcag ccatcaacat attgatctgc aagccgttgc
    51721 acttcacctg cgcgtggcga tgccagtcaa tgttgtagag gctgacaaac agaccgcaca
    51781 gaccgagcgt aatcagccaa atcgcgttaa gcggagagat ttgcacatcg tagcttacca
    51841 gcgacagcgc accgctcacg ccaaccgcgc cagtcagtac agtgaagccc gcggctgccg
    51901 tatacagact accaaccgcg ccgccaattc cggctatcca gccgcttagc gccttttgaa
    51961 aagaaaagag aaatgccaga acagcggcgg cgacaaacca cgccacgccg ctattgatca
    52021 gggaaattgc gctcatttag cctctccact ttgagcctgc tgaaacaagg tgagatcgcc
    52081 aaagtccgtg ttaaaggtca gctcacgctt acgtttgctg acgcgggcga tatcggtgtt
    52141 atccaccaga tgcagggctt tagtcgggca catccgcacg caggccggac cttgttcatc
    52201 aaagctacaa aggtcacatt tgacggcgat cgcgcgaata cccggcaccc agtcaagcag
    52261 cgtactgata cgcgccggag caggcggtgc cggtggcgct ttcggggtat tggcatttgc
    52321 aggaatatcc agcggacggc tgccggaaaa ttcaattgcg ccaaacgggc aggcgatgcc
    52381 gcacagcttg cagcttacgc acaggctttc attcaactgc acggccccat caacgcgggt
    52441 gatggcgtta accgggcaga ccaccgcgca gggtgcatct tcacagtggt gacagagctg
    52501 cggcgcagat tctttttcat ttagcatcac tctcaggcgc ggcattgatt gcaggccgtg
    52561 ctggcgatgc gtctctgaac aggcggcctc acaagtgtgg caaccgatac agagcgtgga
    52621 gtcagcaatt acaaaacgat tcaccaggca ttcctcaggt gattgtcatt tttgacgaaa
    52681 acatgccgat gaaatgtcat tttcgacact catcgacacg cccatcccca aacaggcgta
    52741 acgcctgcaa aacgggcaaa gcctcagctc atgctgccgg gctttgtccc tttaccagtt
    52801 ggcttaaatt caccggcaca ttgttttcaa tagcggtgta taagctgtcg taaacgccag
    52861 cgattttttc gagatagtgt tccagaacct gttcgcgatc gcggatgttg accttgccat
    52921 caatcatgcc gtcaatgctc agttgcgcct gcgcaatccg ctgtttgtct tcgccatctt
    52981 ttgtttcgac gtaatactcg atggtgtggc tgttaaagcc atccgccagg gtgacgtaaa
    53041 tacgaaaatg ttcaaaaagg acgaaacaga gttctttgtc cggcgcgcag ctcatggcgt
    53101 gatgcaggcg cgctttcgat aacgtgatcc cctgaaccag cgaattgcag tagatgtgcc
    53161 actggtcctg taggcgacga tgccgctgtg cgatgtaatc ggctttctcg cttatttccc
    53221 aaatagtcat tgtcaggtta cccgtttaac agagatgcca gctttaagca ttttttgtgc
    53281 caacttttaa tttattatta ttaaagcgat ttttaagatt ttggtgaatt tcgtcgccgt
    53341 gtcgacgtgt catttcgaca tcatcgacat tattcactgc agggataatc aacactggca
    53401 caattattgc ttgtagctgg caatagttaa tgggaggcga tatgcacgaa ataaccctct
    53461 gccaacgggc actggaattg atcgaacagc aggccgcaaa acacggcgca aaacgcgtaa
    53521 ctggggtctg gctcaaaatt ggcgcatttt cttgtgtcga aaccagctct cttgcctttt
    53581 gttttgatct ggtttgccgc ggcagcgtgg cggaaggttg taaactgcac ctcgaagaac
    53641 aagaggccga atgctggtgt gaaacatgcc aacagtatgt gacgctactg acccagcgcg
    53701 tccgccgctg tccacagtgt catggtgaca tgctacagat tgtggcagac gacggtttac
    53761 agattcggcg gatagaaata gaccaggagt gagcgatgtg tacaacatgc ggttgcggtg
    53821 aaggcaacct gtatatcgag ggtgatgaac ataaccctca ttccgcgttt cgtagcgcgc
    53881 catttgcccc ggcggcacgc ccgaagatga aaatcaccgg cattaaagcg cctgaattta
    53941 cccccagcca gactgaagaa ggcgacctgc attacggtca tggcgaagcg ggcactcacg
    54001 caccgggcat gagccagcgt cggatgctgg aagtcgaaat tgacgtgctg gacaaaaata
    54061 accgtctggc tgaacgcaac cgcgcgcgct ttgctgcctg caagcaactg gtgctcaacc
    54121 tggtttccag cccaggttcc ggtaaaacca ccctgctgac ggaaacctta atgcgcctga
    54181 aagacagcgt tccgtgcgcg gttattgaag gtgaccagca aaccgtgaac gatgccgcac
    54241 gcattcgcgc taccggcaca ccagcgattc aggtgaacac cggtaaaggc tgccatcttg
    54301 acgcacagat gattgccgac gccgcaccgc gtctgccact ggacgataac ggtattctgt
    54361 ttatcgaaaa cgttggcaac ctcgtatgcc cggccagctt cgatctcggt gaaaaacaca
    54421 aagtggcggt gctttccgtt accgaaggtg aagacaaacc gctgaaatat ccgcatatgt
    54481 ttgccgccgc ctcgctgatg ctgctcaaca aagttgacct gttgccgtat ctcaactttg
    54541 acgttgagaa gtgcatcgcc tgcgcccgcg aagtcaatcc agaaattgaa atcatcctta
    54601 tttccgccac cagcggcgaa gggatggacc agtggctgaa ctggctggag acacagcgat
    54661 gtgcataggc gttcccggcc agatccgcac cattgacggt aaccaggcga aagtcgacgt
    54721 ctgcggcatt cagcgcgatg tcgatttaac gttagtcggc agctgcgatg aaaacggtca
    54781 gccgcgcgtg ggccagtggg tactggtaca cgttggcttt gccatgagcg taattaatga
    54841 agccgaagca cgcgacactc tcgacgcctt gcaaaacatg tttgacgttg agccggatgt
    54901 cggcgcgctg ttgtatggcg aggaaaaata atgcgttttg ttgatgaata tcgcgcgccg
    54961 gaacaggtga tgcagttaat tgagcatctg cgcgaacgtg cttcacatct ctcttacacc
    55021 gccgaacgcc ctctgcggat tatggaagtg tgcggtggtc atacccacgc catttttaaa
    55081 ttcggcctcg accagttact gcctgaaaac gttgagttta tccacggtcc gggttgcccg
    55141 gtgtgcgtac tgccgatggg cagaatcgac acctgcgtgg agattgccag ccatccggca
    55201 gtcatcttct gtacctttgg cgacgccatg cgcgtgccgg ggaaacaggg atcgctgttg
    55261 caggcaaaag cacgcggtgc cgatgtgcgc atcgtctatt cgccgatgga tgcgttgaaa
    55321 ctggcgcagg agaatccaac ccgcaaagtg gtgttcttcg gcttaggttt tgaaaccacc
    55381 atgccgacca ccgccatcac tctgcaacag gcgaaagcgc gtgatgtgca gaatttttac
    55441 ttcttctgcc agcatattac gcttatcccg acgctgcgca gtttgctgga acagccggat
    55501 aacggtatcg acgcgttcct cgcgccgggc cacgttagta tggttatcgg cactgatgcc
    55561 tataatttta tcgccagcga ttttcagcgt ccgctggtgg tggctggttt cgaacccctt
    55621 gatctactgc aaggcgtggt catgctggtg gagcagaaaa tagcggccca cagcaaggta
    55681 gagaatcagt atcgtcgggt ggtaccggat gccggtaacc tgctggcgca acaggcgatt
    55741 gccgatgtgt tctgtgtcaa cggcgacagc gaatggcgcg gcttaggcgt gattgaatct
    55801 tctggcgtgc acctgacgcc ggattatcaa cgattcgatg ccgaagcaca tttccgcccg
    55861 gcaccgcagc aggtctgcga tgacccgcgc gcgcgttgtg gcgaagtctt gacgggcaaa
    55921 tgtaagccgc atcaatgccc gctgtttggt aacacctgta atcctcaaac cgcgtttggt
    55981 gcgctgatgg tttcctccga aggagcgtgc gccgcgtggt atcagtatcg tcagcaggag
    56041 agtgaagcgt gaataatatc caactcgccc acggtagcgg cggccaggcg atgcagcaat
    56101 taatcaacag cctgtttatg gaagcctttg ccaacccgtg gctggcagag caggaagatc
    56161 aggcacgtct tgatctggcg cagctggtag cggaaggcga ccgtctggcg ttctccaccg
    56221 acagttacgt tattgacccg ctgttcttcc ctggcggtaa tatcggcaag ctggcgattt
    56281 gcggcaccgc gaatgacgtt gcggtcagtg gcgctattcc gcgctatctc tcctgtggct
    56341 ttatcctcga agaaggattg ccgatggaga cactgaaagc cgtagtgacc agcatggcag
    56401 aaaccgcccg cacggcaggc attgccatcg ttactggcga tactaaagtg gtgcagcgcg
    56461 gcgcggcaga taaactgttt atcaacaccg cgggcatggg cgcaattccg acgaatattc
    56521 actggggcgc acagacgcta accgcaggcg atatattgct ggttagcggt acactcggcg
    56581 accacggggc gactatcctt aacctgcgtg agcagctggg gctggatggc gaactggtca
    56641 gcgactgcgc ggtgctgacg ccgcttattc agacgctgcg tgacattccc ggcgtgaaag
    56701 cgctgcgtga tgccacccgt ggtggtgtaa acgcggtggt tcatgagttc gcggcagcct
    56761 gcggttgcgg tattgaaatt tctgaatcag cgctgccggt taaacctgcc gtgcgcggcg
    56821 tttgcgaatt gctgggactg gacgccctga actttgccaa cgaaggcaaa ctggtgatcg
    56881 ccgttgaacg caacgcggca gagcaagtgc tggcagcgtt acattcccat ccactgggga
    56941 aagacgcggc gctgattggt gaagtggtgg aacgtaaagg tgttcgtctt gccggtctgt
    57001 atggcgtgaa acgaaccctc gatttaccac acgccgaacc gcttccgcgt atatgctaat
    57061 aaaattctaa atctcctata gttagtcaat gaccttttgc accgctttgc ggtgctttcc
    57121 tggaagaaca aaatgtcata tacaccgatg agtgatctcg gacagcaagg gttgttcgac
    57181 atcactcgga cactattgca gcagcccgat ctggcctcgc tgtgtgaggc tctttcgcaa
    57241 ctggtaaagc gttctgcgct cgccgacaac gcggctattg tgttgtggca agcgcagact
    57301 caacgtgcgt cttattacgc atcgcgtgaa aaagacaccc ccattaaata tgaagacgaa
    57361 actgttctgg cacacggtcc ggtacgcagc attttgtcgc gccctgatac gttgcattgc
    57421 agttacgaag aattttgtga aacctggccg cagctggccg caggtgggct atacccaaaa
    57481 tttggtcact attgcctgat gccactggcg gcggaagggc atatttttgg tggctgtgaa
    57541 tttattcgtt atgacgatcg cccctggagc gaaaaagagt tcaatcgtct gcaaacattt
    57601 acgcagatcg tttctgtcgt caccgaacaa atccagagtc gcgtcgttaa caatgtcgac
    57661 tatgagttgt tatgccggga acgcgataac ttccgcatcc tggtcgccat caccaacgcg
    57721 gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga aatccattac
    57781 tatttcgata ttgacgatat cagtatcgtc ttacgcagcc accgtaaaaa caaactcaac
    57841 atctactcca ctcactatct tgataaacag catcccgccc acgaacagag cgaagtcgat
    57901 gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct gattaatctc
    57961 cacgagcggg atgatttagc cccctatgaa cgcatgttgt tcgatacctg gggcaaccag
    58021 attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg cgtgctgaaa
    58081 ctggcgcaat gtgaagagaa agtgtttacc actaccaatc tgaatttact gcgccagatt
    58141 gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat ccatcgtctg
    58201 aaagaacggc tggttgatga aaacctcgcc ctgaccgagc agctcaacaa tgttgatagt
    58261 gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa acaagttgaa
    58321 atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac gggtaaagag
    58381 ctgattgccc gtgctatcca taatctcagt gggcgtaata atcgccgcat ggtcaaaatg
    58441 aactgcgcgg cgatgcctgc cggattgctg gagagcgatc tgtttggtca tgagcgtggg
    58501 gcttttaccg gtgccagcgc ccagcgtatc ggtcgttttg aactggcgga taaaagctcc
    58561 ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt gctgcgtgta
    58621 ttgcaggaac aggagtttga acgcctcggc agcaacaaaa tcattcagac ggacgtgcgt
    58681 ctaatcgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgtga gttccgtagc
    58741 gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg cgagcgtccg
    58801 gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg tctggggcgc
    58861 aatatcgaca gcattcctgc cgagacgttg cgcaccttga gcaatatgga gtggccgggt
    58921 aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg cggcaacgtg
    58981 ctgcagctgt cattgccaga tattgcttta ccggagcctg aaacgccgcc tgccgcaacg
    59041 gttgtcgctc aggagggcga agatgaatat cagttgattg ttcgcgtgct gaaagaaact
    59101 aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa acgcacgacc
    59161 ctgctgtcac ggatgaagcg actgggaatt gataaatcgg cattgattta actgcaaatt
    59221 gccggacaga tctgcctgtc cggcatacta ttcacgaggt tttttcggac gatatttttc
    59281 cggcagttct ggcaccggac acttgtcatc gatgagatga cgcacggtta agatcggatg
    59341 acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt cacgctttgc
    59401 aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcgc cgaacacaca
    59461 tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg gctccgctga
    59521 cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca gtttttcacg
    59581 agagatacgc ttgccggaca tactgcctcc acctcattaa gatgcattta tattacaact
    59641 taatcttaaa gggcactatg actctaaaga agaagggtta gccaaccgat acaattttgc
    59701 gtacttgctt cataagcatc acgcaaaagc tgcaaaacag catctttccc ggaaccagca
    59761 tcaagaactc gccgttcgct tcttcccctg aaatgattaa ctccggtatc atgtgcgcct
    59821 tatgtgatta caacgaaaat aaaaaccatc acaccccatt taatatcagg gaaccggaca
    59881 taaccccatg agtgcaatag aaaatttcga cgcccatacg cccatgatgc agcagtatct
    59941 caagctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggtg atttttatga
    60001 actgttttat gacgacgcaa aacgcgcgtc gcaactgctg gatatttcac tgaccaaacg
    60061 cggtgcttcg gcgggagagc cgatcccgat ggcggggatt ccctaccatg cggtggaaaa
    60121 ctacctcgcc aaactggtga atcagggcga gtccgttgcc atctgcgaac aaattggcga
    60181 tccggcgacc agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta cgccaggcac
    60241 catcagcgat gaagccctgt tgcaggagcg tcaggacaac ctgctggcgg ctatctggca
    60301 ggacagcaaa ggtttcggct acgcgacgct ggatatcagt tccggtcgtt ttcgcctgag
    60361 cgaaccggct gaccgggaaa cgatggcggc agaactgcaa cgcactaatc ctgcggaact
    60421 gctgtatgca gaagattttg ctgaaatgtc gttaattgaa ggccgtcgcg gcctgcgccg
    60481 tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag cagttgaatc tgcaatttgg
    60541 gacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt gtgctgccgg
    60601 ttgtctgttg cagtatgcga aagataccca acgtacgact ctgccgcata ttcgttccat
    60661 caccatggaa cgtgagcagg acagcatcat tatggatgcc gcgacgcgtc gtaatctgga
    60721 aatcacccag aacctggcgg gtggtgcgga aaatacgctg gcttctgtgc tcgactgcac
    60781 cgtcacgccg atgggcagcc gtatgctgaa acgctggctg catatgccag tgcgcgatac
    60841 ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca ccgccgagtt
    60901 gcagccggta ctacgtcagg tcggcgacct ggaacgtatt ctggcgcgtc tggcgttgcg
    60961 taccgctcgc ccacgcgatc tggcccgtat gcgtcacgct ttccagcaac tgccggagct
    61021 gcgtgcgcag ttagaaactg ttgatagtgc accagtacag gcgctacgtg agaagatggg
    61081 cgagtttgcc gagctgcgcg atctgctgga gcgagcaatc atcgacacac cgccggtgct
    61141 ggtacgcgac ggtggtgtta tcgcatcagg ctataacgaa gagctggatg agtggcgcgc
    61201 gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc gtgaacgtac
    61261 cggcctggac acgctaaaag ttggctttaa tgcggtgcac ggctactaca ttcaaatcag
    61321 ccgtgggcaa agccatctgg cacctatcaa ctatatgcgt cgccagacgc tgaaaaacgc
    61381 cgagcgctac atcattccag agctaaaaga gtacgaagat aaagtcctca cttcaaaagg
    61441 caaagcactg gctctggaaa aacagcttta tgaagagctg ttcgacctgc tgttgccgca
    61501 tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc tggtgaacct
    61561 ggcggaacgg gcctataccc tgaactacac ctgcccgacc ttcattgata aaccgggcat
    61621 tcgcattacc gaaggccgcc atccggtggt tgaacaggtg ctgaacgagc catttatcgc
    61681 caacccgctg aatctgtcac cgcagcgccg gatgttgatt attaccggtc cgaacatggg
    61741 cggtaaaagt acctatatgc gccagaccgc actgattgcg ctgatggcct atatcggcag
    61801 ctacgtaccg gcgcaaaaag tcgagattgg cccgattgac cgtatcttta cccgcgtagg
    61861 ggcagcggat gatctggctt ccgggcgttc aacctttatg gtggagatga ccgaaaccgc
    61921 taatattctg cataacgcca ccgagtacag tctggtgctg atggatgaga ttgggcgcgg
    61981 aacgtccact tacgatggtc tgtcgctggc gtgggcgtgc gcggaaaatc tggcgaataa
    62041 gattaaggcg ttgacgctgt ttgccaccca ctatttcgag ctgacccagt taccggagaa
    62101 aatggaaggc gtcgccaacg tgcatctcga tgcactggag cacggcgaca ccattgcctt
    62161 tatgcatagc gtgcaggatg gcgcggcgag caaaagctac ggcctggcgg ttgcagctct
    62221 ggccggcgtg ccaaaagagg ttattaagcg cgcacggcaa aaactgcgtg agctggaaag
    62281 catttcgccg aacgccgccg ctacgcaagt ggatggtacg caaatgtctt tgctgtccgt
    62341 accggaagaa acctcgcctg cagtcgaggc actggaaaac ctcgatccgg attcactcac
    62401 cccgcgtcag gcgctggaat ggatttatcg cctgaagagt ctggtgtaat aataattccc
    62461 gatagtcttt tgctatcggg aatattaacg ataactgacg aatcaaataa aaataccctg
    62521 tataatagga aagcttattt tacagggtaa aaccatgcca tctacacgct atcaaaaaat
    62581 caatacccat cactatcgcc atatatgggt cgttggtgat attcatggtg aatatcagtt
    62641 attacaatcc cgcttacatc aactctcttt tttccccgaa accgacttac ttatttctgt
    62701 cggcgataat attgatcgtg ggccggagag tcttaacgtc ctgcgcctgc taaaccagcc
    62761 ctggtttatc tccgttaaag gcaaccacga agcaatggcg ctggatgcgt tcgcgaccgg
    62821 cgatggcaat atgtggcttg ccagcggtgg tgactggttt ttcgatttaa atgattcaga
    62881 gcaaaaagaa gctacagatc tgttgctgaa attccatcac cttccacata ttattgaaat
    62941 cactaacgac aacataaaat atgtcatcgc acatgcagat tatccgggga gtgaatatct
    63001 ctttggtaaa gaaatagcgg agagcgaatt actctggcct gttgatcgtg tgcagaaatc
    63061 gcttaatggc gagttacaac aaataaacgg cgctgattat tttatatttg gacatatgat
    63121 gtttgataac attcagacgt tcgctaacca gatttatatt gataccggat cgccgaaaag
    63181 cgggcggctg tcattttata aaataaagta atgaggccgg gtaaagcaaa gccactaccc
    63241 ggcacttttt attagcgctt accttccgcc agcagcggcg ggatgctcgg caccattggc
    63301 gcgtcatcaa tatctttctg cgtcatgcgg aacgcttcgg gataatgttc gcggctggta
    63361 cggcgcagcg gttcggtatc gcgccaggta taaaggcaat gctggcactg atataccgtc
    63421 cagacatctt tcaccggcga tttcgccatc acttcaatct gttcatcggc acaacgtgga
    63481 caaatcatct tgttctcctt atttacgtgc ggccagcata gcggtcagtt tttcagccca
    63541 ggctttggtt tccggcaaat ccaccaccgg ctggctgtag tgaccacggt tgtccggggc
    63601 gacaggcgtg gtggcgtcga taatcagctt gtcggtgatc cccgccgggc ttgagccagg
    63661 gtcgagttcc agcacggaca tattcggcaa ctgcaccaaa tcccctgccg gatttacttt
    63721 cgaggagagc gcccacatca cctgcggcag gttgaacggg tcaacgtctt catcgaccat
    63781 aatcaccatc tttacgtagc ccagaccgtg cggcgtggtc atcgcacgca ggcccaccgc
    63841 gcgggcaaag ccgccgtagc gttttttggt ggagataatc gccagcaggc cgtgggtgta
    63901 catggcgttt accgcctgca cttccgggaa ctcggctttc agttgttgat acagcggcac
    63961 acaggtggct ggccccatca ggtagtcgat ttcggtccac ggcatgccga ggtacagcga
    64021 ttcgaaaatc ggcctggtgc ggtaagagac tttatcgata cgcaccacgg tcatgttacg
    64081 cccgccggag tagtgcccgg taaactcacc gaacggccct tcgatttcgc gtttgcggct
    64141 ttcgataacc ccttcgagga tcacttctga accccacggc acatcaaaac cagtcaatgg
    64201 cgcggtggcg atcgggtacg ggctttcgcg cagcgcgcct gccatttcat actcagactg
    64261 atcgtatttc agcggcgtgg cgcccataag ggtgatgatc ggatcgttac ccagggtgat
    64321 ggcaatcggc agatcttcac cgcgctcttc cgctttatgc agatgcaggg cgatatcgtg
    64381 catcggcacc ggttgcaggc cgagcttacg cttgcccttc acttccatgc ggtagatacc
    64441 gacgttctgc ttgccgaagt tatccgggtc gagcggatcg cgggaaacca cgcacgcttt
    64501 gtcgagatag aaaccgccat caccgtcgtt taaacgaaac agcggcagga tatcgaacag
    64561 attaatctcg tcaccatcga cggtgttctg cgcccaggct ggattggcgc ggcgctccgg
    64621 ggcgatcggg aagttatccc agcggcggat aaactcatca atctgttttt taaccggggt
    64681 gtttggcggc aggcccaagg aaatcgcgtg gttctgccag gaaccgatgg tattcatcgc
    64741 cacgcgggca tcggtaaaac cgcgaatatt atcaaaccac agcgccggtg caccatcgcc
    64801 gatacgcccg gtggcgttgg cagcggcagc cagatccggt tccgcgttca cctcttcgct
    64861 gattttcagt aactggccgt ggtcatcaag cgcctgtaaa aagctgcgta aatcatcaaa
    64921 tgccattatt cattctcctg agaaaaattc cgggcctgcg gcaatccttg ccagcgcctg
    64981 gcgtagggat gttcaaggcc aaattgatcc agcacgcggg ctaccacgtg gtggacaatg
    65041 tcatctaccg tttcgggatg gttataaaag gcaggcatcg gcggcaccat cgccacgccc
    65101 atgcgtgaaa gtgcgagcat attttcgaga tggatggtgc taagcggcat ttcacgcggc
    65161 accagcacca gtttgcggcc ttctttaagc acgacgtccg ccgcgcgccc taccaggcca
    65221 tcagcgtaac cagcgcggat accggcgagc gttttcatac tgcacggaat aacgatcatg
    65281 ccgtctgtac gaaaggaacc tgaggagatg gtcgccgcct gatccgccgg gttatggctg
    65341 aagtcagcga gggcagcaac atcgcgggcg ctgtaaggcg tttccagttc aatggtggtt
    65401 ttcgcccact tcgacatcac cagatgagtc tcgacattcg gcatctcccg cagcgcttgc
    65461 agtaatgcca caccaagagg cgcaccggta gcccctgtca tcccgacgat cagtttcatt
    65521 gcttactctc ttaattgttc gtatacgaac attattatta aaatacgcct ctgttctcat
    65581 tgcggcaagc actgaaaagt aacaacatcc ccttcgtcac tgaaaaaccg ctatgatagc
    65641 ggcagatagt ttgcccggag tttacatgac gttacgaaat aaagcgttcc atcagttacg
    65701 acagcttttt cagcagcaca cggctcgctg gcagcacgag ttacctgacc tgaccaaacc
    65761 acagtatgcg gtgatgcgcg ccattgctga taagcctggt atcgaacagg tggcactgat
    65821 agaagcagca gtcagcacca aagcaacgtt ggcagaaatg ttggcaagaa tggagaatcg
    65881 tggcttagtc agacgtgaac atgatgccgc tgacaagcgg cgtcgctttg tctggctgac
    65941 tgccgaagga gaaaagatac ttgctgcggc gataccgatt ggtgacagcg tggatgagga
    66001 atttttgggg cgtttgagtg cagaagagca ggagctgttt gtgcagctgg tgcgcaagat
    66061 gatgaacaca taggatgcaa attgccgggt aggacgctga cgtgtcttat ccaggcgaca
    66121 aaaacacaag cttcccaaac aaaagaaaaa ggccagcctc gcttgagact ggcctttctg
    66181 acagatgctt acttactcgc ggaacagcgc ttcgatattc agcccctgcg tttgcaggat
    66241 ttcgcgcaaa cggcgcaggc cttcaacctg aatctggcga acacgttcac gggtgaggcc
    66301 aatttcacga cctacatctt ccagtgttgc cgcttcgtac cccagcaaac cgaatcgacg
    66361 tgccagtact tcacgctgtt tggcgttcag ctcgaacagc catttgacga tgctctgctt
    66421 catatcgtca tcttgcgtgg tatcttccgg accgttctct ttttcatcgg ccaggatgtc
    66481 cagcaacgct ttttcggaat caccacccag cggggtgtct accgaggtaa tgcgctcgtt
    66541 aagacgaagc atacggctga cgtcatcaac tggcttatcc agttgctctg cgatctcttc
    66601 cgcacttggt tcatggtcca gcttatggga caactcacgt gctgttcgca ggtaaacgtt
    66661 cagctccttt acgatgtgaa tcggcaaacg aatagtacgg gtttggttca taatcgcccg
    66721 ttcaatcgtc tggcgaatcc accaggttgc gtatgttgag aagcggaaac cacgttccgg
    66781 gtcaaacttc tctaccgcac ggatcagccc caggttgccc tcttcgataa ggtccagcaa
    66841 cgccagacca cgattgccat aacggcgggc aatttttacc accagacgca agttactctc
    66901 gatcatccgg cggcgagagg cgacatctcc acgcagtgcg cgacgcgcaa aataaacttc
    66961 ttcttcggcc gttaacagtg gtgaataacc aatctcacca aggtaaagct gagtcgcgtc
    67021 caacacacgc tgtgtggctc cctgcgataa cagttcctct tcggccaaat cgttatcact
    67081 gggttcctct tctactaagg ccttttcgtc aaaaacctca actccgttct catcaaattc
    67141 cgcatcttca tttaaatcat gaactttcag cgtattctga ctcataaggt ggctcctacc
    67201 cgtgatccct tgacggaaca ttcaagcaaa agcctggttc cgccgattta tcgctgcggc
    67261 aaataacgca gcgggtttac ggatttcccc ttgtaacgaa tttcaaaatg caagcgtgtt
    67321 gaactggttc cggtgctacc catggttgct attttttgcc ccgccttcac ttcttgttgt
    67381 tcccggacca gcattgtgtc gttatgggcg taggcactca ggtaatcatc attatgtttg
    67441 atgataatca gattaccgta gccgcgcagc gcgttaccgg cataaacaac acggccatct
    67501 gcggtcgcga taattgcctg tcctttgctg cctgcgatat caatcccctt gttgcccccc
    67561 tcagaagcgc caaaggtttc gatcactttg ccctcagtcg gccagcgcca ggtggagata
    67621 ggcgtactgg ttgatgtact gctgacagtc ggctcggttg tgcttgctgt tggtaccgtt
    67681 acaggcgctg tgaccgtggt cgcagttggc ttgttgttcg gcaacatttt gttagcactc
    67741 tgttcacccg aagactcaga atacgtaatt gtcggttgcg acgcaacagc aacggtggaa
    67801 ttttgtgcag gcttgatcac aactccttgc tctgctgcgt cggcctgggt aatggcattt
    67861 ccgccagtga ttggcgtacc ggaagcatta cccacctgta aggtctgacc gacgttcagc
    67921 gcgtatggtg cctgaatatt gttgcgctga gcaaggtcac ggaaatcgtt gccagtaatc
    67981 caggcgatat agaaaagcgt gtcgcctttt ttcacggtat aggtactgcc gctataactg
    68041 cctttcggaa tgttcccata ctgacggtta tagacgatgc gtccgttttc catctgtacc
    68101 ggctgctgag ctactggctg cactggctgg atttgcggtt gttgagtagc ctgaatttgt
    68161 ggctgctgca ccggctgaat ttgcggttgc tgcgctgtag acgtcgtccc cattttcggc
    68221 ggcggcgtaa tcaacatacc agaattggta tgtgcaggcg cattgccatt aacggagctg
    68281 accggtgccg gtggatttga agtgtcagaa cagcctgcca gccatagcga aaccagtgac
    68341 aaagccgcaa tgcggcgaac ggtgaatttt gggcttcccg cgctcattta tcccccagga
    68401 aaaattggtt aataaccagt gacataatta ccgtgcaagg caccatactg aacactggaa
    68461 aagatgttca cgatacgctg acctgcggca aaataaccag gaaaaatcca ggtatttcct
    68521 cacgttttaa gccagctcac ccttcactaa agggacaaag cgcacggcct ccacggtatc
    68581 gataataaat tcgcctcccc gacgacgcac ccgtttcaaa tactggtgct cctcccctac
    68641 gggtaagacg agaatcccgc cttcgtccag ctgcgtcatt agcgcagttg gaatttccgg
    68701 cggtgccgcc gtaacaatga tagcgtcaaa cggcgcacgt gcctgccaac cttgccatcc
    68761 atcgccatga cgggttgaaa cattatgtaa atcaagattt ttcaggcggc gacgcgcctg
    68821 ccactgcaag cctttaatcc gttcaaccga gcaaacatgc tggacaagat gcgccaggat
    68881 tgccgtttga tatcccgaac cggtgccaat ttccagcacc cgcgactgcg gcgtcagctc
    68941 gagtaattcg gtcattcgcg ccaccatata cggctgcgaa atcgtctgcc cctgacctat
    69001 cggcaaggcg atattgtccc aggctttttg ttcaaacgct tcatcaacga atttttcacg
    69061 cggcacggcg gcaagtgcat tcagcacctg ctcatcctga ataccttgcg cacgtaattg
    69121 atccagaagt gcttgtacgc gtctgcttac cattgcgtgc caactcccac gctgtttaac
    69181 cagtctgaaa ccacatcttg cgcgctatgc gcagttaaat ccacatgcag cggcgtgatg
    69241 gagacatagc cctcatctac cgcagcaaaa tcggtccccg gaccggcatc acatttaccg
    69301 cccggcgggc caatccagta cagcgtattg ccgcgcggat cttgctgcgg gatcacctga
    69361 tctgccggat gtcgtgtacc gcagcgcgtc acgcgaatac ctttgatttg atccaagggt
    69421 aaatccggaa cattaatatt aagaatacgc ccggtgcgca gcggctcttt acacagtgcg
    69481 cgcaaaattg aacaggttac cgccgcggca gtgtcgtaat gtttatgccc gtcaagcgag
    69541 acggcaagcg ccggaaaacc taaatgacgg ccttccatcg cggcggctac cgtaccggaa
    69601 taaataacat catcccccag attcggcccg gcgttaattc cggacacaac aatgtccggg
    69661 cgcggacgca tcagagcatt cacgccaaga tagacgcaat cggtcggggt tcccatttgc
    69721 acagcaatat caccattttc aaaggtaaac gtgcgcaggg aggattccag tgtcagagaa
    69781 tttgaagcgc cgctgcggtt acgatcgggg gcgaccacct gaacgtcagc aaactcacgc
    69841 aaggctttcg ccagcgtttg tataccgggt gcatgtaccc cgtcatcatt actcagcaat
    69901 atgcgcataa tcacctgttg tgttgataag ttccctgaca acgctggttg caaaactacc
    69961 cgccggaagc cagaaacgga tttctacggt gacgtcatcc caccaattcc agcttaattg
    70021 ttgcggatac agcagcatcg ctctgcgcgc ggcttcaact ttttcgcgca ccagtaaagc
    70081 ttgtaattca gtttctgcgg cgacagctgc ctgttcgaat gccagcgctt cacgctgagt
    70141 tccccattca ccactgcccg gcaatgcggc ggttatcatc agctctttat cgttgacgcg
    70201 acgctgtaat tccaccagtt cttcggtggt tgcaacaaac cagctaccac gtccggctaa
    70261 ttgtagcgca tcgccgtcaa caacttgatt aacgtctgct tttttgaggc gctcagcaac
    70321 aatctgatta aacaacgcac tgcgggctgc cgacaaccaa aaactccgtt tattgcgatc
    70381 gcgcaccgga gtattggttt gcgcccagcg cagcgcgccc tgcaagttgc taccgccaat
    70441 cccaaaacgt tgggcaccga agtagttcgg tacacctttt acgcaaatat cgatcagacg
    70501 ttgttcaacg tcatcgcgat tgctcacttc gcgcagaacc agggtaaagg cgttaccttt
    70561 cagcgcccct aaacgcagct tgcgcttgtg ccgcgcatac tccagcacct ggcagccttc
    70621 caattgaaag gcgctcagat cgggcatttc cttgcccggc acgcgagcgc ataaccactg
    70681 ttctgtaaca gcatgtttgt ctttttgccc agcaaagctg acttcacggg catgaatttt
    70741 caggaatttc gccagtgcat ccgccacaaa acgggtattg cagccgtttt tgaggattct
    70801 aaccagaata tgctcacctt caccatcagg ctcaaagccc aaatcttcca ccaccacaaa
    70861 gtcttccgga ttggctttca gcagcccggt gccttgcggt ttaccgtgga ggtaagtgag
    70921 attatcaaac tcaatcattt tgttgcctta atgagtagcg ccaccgcttc acaggcaatc
    70981 ccttccccac gtccggtaaa tccaagtttt tccgtagtag tggctttcac gttaacatca
    71041 tccatatggc agccgagatc ttcggcaata aacacgcgca tttgtggaat gtgcggcaac
    71101 atcttcggtg cctgagcgat gatagtgaca tcgacgttgc caagggtata acccttcgcc
    71161 tgaatacgac gccaggcttc gcgtagcagc tcgcggctat cggcaccttt aaatgccgga
    71221 tcggtatccg ggaacagttt gccgatatcc cccagcgccg ccgcgccaag caatgcatcg
    71281 gtcaacgcat ggagcgccac gtcgccatca gaatgcgcca gcaatccttt ttcgtaagga
    71341 atgcgtacgc caccaatgat aattgggcct tcaccgccaa aggcgtgtac gtcaaaaccg
    71401 tgtccaattc gcattatgta ttctcctgat ggatggttcg ggtgaggtaa aactcggcca
    71461 gtgccaaatc ctccgggcgc gtgactttaa tgttatccgc acggccttcg accaactgag
    71521 gatggaatcc gcaatattcc agcgccgagg cttcgtcggt aatagccgcg ccttcattta
    71581 gagcgcgcgt cagacagtca tgtaacagct cacgagggaa aaattgcggc gtcagcgcgt
    71641 gccataagcc gttgcgatca acggtatgag caatggcatt tttgcccggt tcggcacgtt
    71701 tcatagtatc gcgcactggt gcggctagga tccctcccgt gcggctggtt tcgctcaacg
    71761 ccaacaatcg cgcgaggtca tcctgatgca gacaaggacg agcggcgtca tgcaccaata
    71821 cccactgcgc gtcgccagcg gctttcaaac ctgccagcac ggaatcggca cgctcatcac
    71881 cgccatctac aacggtgatt tgcggatgat tcgccagagg aagttgtgca aaacggctat
    71941 cgccaggact tatggcaatg acgacacgtt tcacccgggg atgcgccagc agcgcataca
    72001 ccgagtgttc aagaatggtt tgattaccga ttgagagata ttgcttagga cattccgttt
    72061 gcattcgacg gccaaatccg gccgccggaa ccacggcgca aacatccaaa tgagtggttg
    72121 ccatgttaat tcccgggctg atttatcgat tgttttgccc cgcagactgt gcgcgcttcg
    72181 acgcgtcagg caccagacga taaaaagttt cgcccggcct ggtcatgctg agttcattac
    72241 gcgcacgctc ttcgagcgcc tcctggccgc cattgagatc gtcaatttcg gcaaaaagtt
    72301 gatcgtttcg cgctttaagt ttcgcgtttg tagcttgctg tgccgccaca tcatcattga
    72361 cgcgggtata gtcatgtata ccgttcttac cgaaccacag cgaatactgt agccagacca
    72421 gaatagccag caacagcagc gttagtttac ccatcctgcc ccctgaaaaa cggcatcatc
    72481 atcccatgca tccgaagacg actctacatc ctctgttggg gataccgcga caacgcgggc
    72541 aaatgtacca catttgtcca ttgttacgta tacccagggc gtgcagaaca taatctcatt
    72601 attagttacg gtttgaatta tgaacagagg agacaagaaa gtacaaatta gcccagtagc
    72661 cacataaaca gtgcgccaaa cataatgcct actgtcatca gggtgaaaac aatactgtag
    72721 cgtagctttc cgtccatcaa tgaatgcagc gcaatcccca ccactaccgc aacgggcatc
    72781 agcgccagaa agaaaggcca ggtgtagata aagaagaaca gcgtgttaga gccataaatc
    72841 aacatcggca tcgccagcgc aaataaccag gagataaaac cgaccacggc accaggcagt
    72901 gaccatgtgg tttcttcatc ctcagtaagg ctgtcgttat ttgttagtgt aatgttatgg
    72961 ctattacgca tatttgatcc tgttactttg acgaaccggg catggaaacc cggtggtgtc
    73021 tcaggatctg ataatatcgt tctgtctcaa cagatctaat aattgctgta ccaaatttgt
    73081 tactaattgt tcaccattga gatgaatttc tgccgattca ggcgcttcgt aaacggaatc
    73141 tattcccgta aagttgcgca gttcaccggc acgcgctttc ttatataagc cttttggatc
    73201 gcgggcttcg caaatcgcca gcggcgtatc gacaaacact tcgataaagc gcccttctcc
    73261 tacgcgttcg cgaaccatct ggcgttcggc gcggtgtggc gagataaatg cggtcagcac
    73321 caccagtccg gcttcaacca tcaaattcgc cacttcaccg acgcgacgga tattctcttt
    73381 acgatcggca tcgctaaaac cgagatcgct gcataatccg tggcgaacat tgtcgccatc
    73441 cagcagatac gtactgacgc cgagtttatg taacgcctcc tccagcgccc cggcgaccgt
    73501 tgatttaccg gacccggaga ggccggtaaa ccacagcact acaccacgat gaccgtggtg
    73561 tagctcgcgt tgttgcacag tgaccggatg gctatgccag acgacgtttt cgtcatgcag
    73621 cgccattatt tctcccccag caaatcgcgc gcgccccagt gtggaaagtg gcggcgaacc
    73681 agggcattca attccagttc gaatgcacta aattcagatg gcgcagcagt tgcctggcta
    73741 actggctcat gcaccatacc ggcacctacg gtcacattgc tcaggcgatc gataaaaatc
    73801 agcccaccgg taaccgggtt ttgctgataa cgatctaaca ccagtggctc gtcaaaagtg
    73861 agatccacca ggccgatgcc gttcagcggc aggttttcaa cttcgcgttg ggtaaggtta
    73921 ttaatatcaa cctgataacg aatgccatca acacgagaac gcgtcttctt accggcaatt
    73981 ttgatgtcgt aactctggcc cggggaaagc ggctgttccg ccatccatac cacatccacc
    74041 gacgcgctct gcacagctgg taacgcttcg tctgccgcca gcagcagatc gccacggctg
    74101 atgtcgatct catccgtcag caccagggtg atagcttctc cggcaaaggc ttcttcgcga
    74161 tcaccatcaa aagtcacgat ccgcgcgacg tttgattcca caccagaggg cagcactttt
    74221 acacgttgcc cgacttccac gcgaccggat gccagcgttc cggcgtagcc acgaaaatcg
    74281 agatttgggc ggttaacgta ctgcaccggg aagcgcattg gctgggcatc caccactcgc
    74341 tgaatctcca cggtttccag cacttcgagc agtgtcggac cgctgtacca cggcatactt
    74401 tcactttgcg aagccacgtt gtcaccttcc agtgcggaga gcggcacaaa gcggatatcc
    74461 agattacccg gcagctgccc ggcaaaggtc agataatctt cacgaatacg ggtgaacgtc
    74521 tcttcactgt aatccaccag atccattttg ttgatcgcca cgaccagatg tttgatcccc
    74581 aacagtgtgg agataaaact gtgacgacgg gtttgatcga gcacgccttt acgggcatcg
    74641 atcagtaaga tcgccagttc acatgtcgat gcgccagtcg ccatattgcg ggtgtactgc
    74701 tcgtgccctg gagtgtcggc gataataaat ttacgcttct cggtagagaa atagcggtag
    74761 gccacgtcaa tggtgatgcc ctgttcacgc tcagcttgca ggccgtccac cagcagagcc
    74821 agatccagct tttcgccctg ggtgccgtga cgcttactgt cattatgcag cgatgagagc
    74881 tgatcttcat agatttggcg ggtatcgtgc aacagacgac caatcagggt acttttgccg
    74941 tcatcgacgc taccacaggt cagaaaacgc agcaggcttt tatgttgttg cgcaatcatc
    75001 caggcttcga cgccgccttc attggcgatt tgttgtgcaa gtgcggtgtt catcttaaaa
    75061 atacccctga cgttttttca gctccataga accagcttgg tcgcggtcaa tcacgcgccc
    75121 ctgacgttca ctggtggtgg aaaccagcat ctcttcgatg atctccggca gcgtttgtgc
    75181 atttgactcc accgcaccgg tcagcggcca gcagcccagc gtacggaaac gcaccatccg
    75241 ttttttaatc acttcgcccg gttgcaggtc gatacggttg tcatcaatca tcatcaacat
    75301 accgtcgcgt tccagaaccg gacgttccgc agcgagatac agcggcacaa tgtcgatatt
    75361 ttccagccag atgtattgcc agatatcctg ctcggtccag ttagagagcg ggaaaacgcg
    75421 gatgctttcg cctttgttaa tctgcccgtt atagttgtgc cacagctccg ggcgctgatt
    75481 tttcgggtcc cagcgatgga agcgatcacg gaaagagtag atacgctctt tagcacggga
    75541 tttctcttcg tcgcggcgcg caccaccgaa ggcggcatca aaaccgtatt tattcagcgc
    75601 ctgcttcagg ccttcggtct tcataatatc ggtatgtttc gcgctgccgt gcacgaatgg
    75661 attaatcccc atcgccacgc cttccgggtt ttgatgcacc agcagctcgc agccgtaggc
    75721 tttcgccgta cgatcgcgga actcatacat ctcacggaat ttccagccgg tatcgacatg
    75781 cagcaacggg aaaggcagcg tacctggata aaacgccttg cgcgccagat gcagcatgac
    75841 gctggaatct ttaccgatag agtagagcat caccggattt gagaattctg ccgccacctc
    75901 gcgaataatg tggatgcttt ccgcctccag ttgccgcagg tgagtaagtc gtatttgatc
    75961 cataaccgtt cctttgcaat accgctattt tcttgccatc agatgtttcg actataggga
    76021 gcgtaagaga acgaatgaaa ttaccaatta gaatgagtag ttccttaacg gaataacgat
    76081 ttggcaaagc taatatcaaa aagtgcttaa ggcaccggat ttcgggcgtt taggaagatt
    76141 tgaaattgtt ttagcgcagc ggcagtttca tactatggcg gtaaaaaaat ttgcatggta
    76201 tttaaggact cactatgttt tccgcattgc gccaccgtac cgctgccctg gcgctcggcg
    76261 tatgctttat tctccccgta cacgcctcgt cacctaaacc tggcgatttt gccaatacac
    76321 aggcgcgaca tattgccact ttctttccgg gacgaatgac cggaacaccc gcagaaatgt
    76381 tatctgccga ttatattcgg caacagtttc agcaaatggg ttaccgcagt gatattcgta
    76441 cgtttaatag ccgatatatt tataccgccc gcgataaccg caaaaactgg cacaacgtga
    76501 cgggaagtac ggtgattgcc gctcatgaag gcaaagcgcc gcagcagatc attattatgg
    76561 cgcatctgga tacctatgcc ccgcagagcg acgcagatgc agatgccaat ctcggcgggc
    76621 tgacgttaca aggaatggat gataacgccg caggtttagg tgtcatgctg gaactggcag
    76681 aacgcctgaa aaatacgcct accgagtatg gtattcgatt tgtggcgacc agtggagaag
    76741 aggaagggaa attaggcgct gagaatttac tcaagcggat gagtgacacc gaaaagaaaa
    76801 atacgctgct ggtgattaat ctcgataact taattgttgg cgataaattg tatttcaaca
    76861 gcggtgtaaa aacccctgaa gcagtaagga aattaacgcg cgacagggcg ctggcaattg
    76921 cgcgtagtca tggaattgcc gcaacgacca atccgggttt gaataaaaat tatccgaaag
    76981 gcactggatg ttgtaatgac gcagaaatat tcgacaaagc gggcattgct gtactttcgg
    77041 tggaagcgac aaactggaat cttgggaata aagatggtta tcagcaacgc gcaaaaacag
    77101 ccgcattccc tgcgggaaat agctggcatg acgtaagact ggataatcag caacatattg
    77161 ataaagcact tcctggaaga atagaacgtc gctgccgtga cgttatgcgg ataatgctac
    77221 cgctggtgaa ggagttggcg aaggcgtctt gatgggttgg aaaatgggag ctgggtgttc
    77281 taccgcaggg gcggggaatt ctaagtgata tccatcatcg catccagtgc gcccggttta
    77341 tccccgctga tgcggggaac acagcggcac gctggattga acaaatccct gggccggttt
    77401 atccccgctg gcgcggggaa cacaaaccga aacacacgat caatccgaat atgagcggtt
    77461 tatccccgct ggcgcgggga acactttggt gacagttttt gtcactgttt tggtgacggt
    77521 ttatccccgc tggcgcgggg aacacactaa gcatacatat ctgtttttaa acaaatttat
    77581 tccacatcaa caatctacca actaaattca aacatttcct tatttttaaa gaacacataa
    77641 cctattgatt atcaacagga agaaaagaaa ccaaacgtaa cccatccaaa tccaccggaa
    77701 tacgtctgtt ttctccccag gtctgaaatt caaaacccga ctcggtattg gtcgcccagg
    77761 ccatcaccac atttccgcaa ccagccagtt gggtaatttg ctgccagatc atctcccgaa
    77821 tacgttttga tgtatcacca acatacacac cggcacgcac ttccagtagc cagattgcga
    77881 gccgtccacg taagcgcgga gggacatttt ctgtaacaac cacgaccatg ctcatccgcc
    77941 gcgcccccgg tgaccactat cacccagcgt ttcaggttca gggatggcag gcggtaacat
    78001 atccggcgcg ggttgtggtg gttcaatttc acctgcagca aggactttct caattaacgg
    78061 tattaatttg cccgttaact tagtgctacg gaaaatatcg cgacaggcta atctgacttc
    78121 tttatcaggt tctgcgggtt gcctcgctgc tatttcaaat gcctttggca caaccgaatc
    78181 aaatttaatg atatcggcta tgtcataaac aaatgaaagc ggtttgccac tatgaataaa
    78241 tccaatagcg ggcgcatatc ccgcggctaa tactgccgct tcagaaatac cgtacagaca
    78301 tgatgtggca gcactgatgc agcgattcac aacatcgcct ttttcccagt ctttaggatc
    78361 gtatttgcga ccattccatt tcacaccata ttgtttcgcc agtaatgcat aggtctggcg
    78421 aacgcgggat ccctcaattc cccgtagctg atccactgaa cggcgagctg gcggtggctc
    78481 acgaaaacgt aattcataca ttttgcgcac caccttcagg cgtagatctt ccgttaaagc
    78541 cagctttgcc tggtagagta atttatctgc ccgcgcccct ccgggttgtc cggaagagta
    78601 aacgcgaacg cccgcttcac cgacccagac cagcagtgtt cccaccgtgg cggccagatg
    78661 caccgccgcg tgggaaactc tcgttcccgg ttcgagcata atgcaggcga ccgatcccac
    78721 cggaatgtgc gtgcggatcc cggttttgtc gatcagcacg aaagcgccgt ccagtacgtc
    78781 gatttgaccg tactggagga agatcataga ggtgcgatct tttaacggga tcggactcag
    78841 tggtacaaac gtcacacctc tgctccgggt ttgatcagca tcagaccaca gccgaacgcg
    78901 cggcttttgc catacccctg actgagacgt tgcagaaata acccgggatc ggtgaccgta
    78961 agcatccccg tataatccac gctactgaac tggatcagtt gccgggagtt ttcccgccgc
    79021 agttgctgtt gtcggtaggc atcaaccgag gtatccagca gtgtaaaacc gcttctctcc
    79081 ccctgcgctg ccagccagtc cagcgccgcc tgttgttgat gcaaccagac atcacttcct
    79141 tccgcctgcc ccctcacctg ccgtttcgcc tccatcagca gatcgtggcg cttgcccgct
    79201 ttacagattg ttggattcgc ccgcaggttg aaacaaagtt gttgtccggt acgcagctcg
    79261 ggcacaaatg accggcattc gatggtgaac gtttcacttt ccgccgggcg ttcctgcgac
    79321 agtacaaaaa agcgaaacgc gccctggagt tcttcccgac gataaagaaa ttgcctttct
    79381 ttgccgccag ggaagagatc ccacagccac tgatgcatca catattcccc gcgatccacc
    79441 aaatgcagca actgcgcagg cgaaagctgg ccggtatgta aggttattct tgagaggtac
    79501 atggttcctc cttgctgagc cacggcccct gattgatggt gcgttcccca aacagccact
    79561 gctgacgatt taaaggaaca tcccggcgac gtaatatttt gctcgcgacc aggccgtcgt
    79621 gttccccttc ccaccagcat tcatcctgaa gtttcgggag tgagactttc agttcgcgga
    79681 aactatcctg atactgttgg tatgcgttac gtaagacatc agacgcgttg ccttcgagca
    79741 gtaacggcgc aagtggtaac gccagcggat gactttttcg ccccagataa agcggaaaaa
    79801 ccggatgacg taaaccgtcc tgcaactgtt caaggctgta aggcgcatcg ggggttgttg
    79861 ccaccgccac catccaccag gcatcggtgt agtagtcgcg ccgggagata atcgcgctca
    79921 gaagatcagg ggcgctcaac tcttcgcgac ggctgaaata acgcgcttta cgcacctctt
    79981 ttggcatctg gaccgtgtga taatcccgtg cccagcgcgg gttacggctg gcgcaaacca
    80041 ccagtgaata gtggcggtta aacgcgttta atcgttcggt atcatcacgc cgaatcccta
    80101 ccccggcagc cagcagcccc agcaatgctg agcgcgaagg cagttcatgg gtatgacgca
    80161 cttcgccggg ggcatcgacg ccccaggatg ccattggccc atgaagctga aaaatcaaat
    80221 attggctcat tagccgcccc ttacgcgcag ataaagtcca gcacgtcctt catgcttccc
    80281 tgtttgttca tcacgtcaaa gcttgcgcat tcggtcttct gttcatagac cgtattcata
    80341 ttttcgcgaa gcgttgtaat acgctgcacc gccacatcta actgccgggt gccattaatg
    80401 ggttcataga aagccgccgc cagagaacgt ggttgttcgg tgcctttttc tgccagcgcc
    80461 caggaggcgt aggcacggct ggcaaagctg ttctgtttgc cggttgggga gactttaagt
    80521 gcggcttccg taaaggcgcg caaggtctga ttagctaacg cttcgtcacc gccgaggttt
    80581 tcgaccagca gatctttatc gatgcagata taggtgtaga acagcgcaga accgaatccg
    80641 gtttcaccaa gatgcccggc accggcatct tcagaagcct ggcgcaaatc atcaacggcg
    80701 gtgaaaaaat catcttcgac aatcgtttca ctgacaccaa atgcatgcgc gacctggcag
    80761 gcggcttcaa cattaaattc gggtttattc gccagcatac gaccaaacat agcgatatct
    80821 actgccatgc gatctttacg taacaaagcg agatcttcct cttttggcgc gcgcttttct
    80881 tcggccagtt gatgggccag cgcttttacg gcgtcaaatt ctgccgggct gatatggact
    80941 aattgttcag tttcggcgtt agtgagcgga tcttttggtt ttttgtcgtt tttagctttc
    81001 ccaagataat ccgcaatttt tgccgcccat tcgatggctt ttttctcttc gatgcctttc
    81061 tcaatcagga tagttgccgc ctcacgcgca atacgcccac tgcgaatacc aatatggccc
    81121 gccagtgcct gttcaaaaag tgcagaagtg cgccacgcac gtttcagact ttgcgaggaa
    81181 acgcgcagtc gcgttgctcc acccaggacc acggttttcg gcgctccggt gtcatcacgg
    81241 ttaaggttgg ctgcagggta agcggttaac aaatgaagct gaataaatgt cgtcatgaga
    81301 tagtccttta ttgattttgt tcgttatcga cgtcgccagc ctggtaatat tccagcgccc
    81361 agcggatacg gataaattcg gttggccgct gatgacggcg gtgatgattg agcagatcgt
    81421 cgctctcctg gcaccagcgg aagacaccct ctgccagaga gtcaagatta acggaaccgt
    81481 ttaataatct gactacgcgg cgtaactggc gcagtaattc atccggtgtt tttactgccg
    81541 acaggcgagt aaaacgcccc tttgacatta ccgccgccag ctgcgcagca aaaggcagcc
    81601 gttcatcaat ggctttaaca ttagcgctaa gtgcggctat cagcgccagt gcggtaatac
    81661 gccattcagg ttcatcctgc cactttattt gtctgttctt cagaaacagg cgaaatccat
    81721 ccgtcagaca aacatcatta accgtcgtac tacgccgcag actggcacgt tcgccgcgtt
    81781 tctcctgcaa ttcctcatgc catttgcgca gcgtggcttt gtgctcctct tttacaatac
    81841 tcattcagca gcctcctgct ttttctccct ggcggcttta gcactttgct tctccgccga
    81901 tgttgtaaaa tatttcttgc gcgcggtcat gacgcgttcc aaatcaacgg gctcataagg
    81961 attggtgaat acacgctcgt caaaatcctg acgtgcgaat aaccaaattt ccttttgcca
    82021 tttgccgagt aattcatccg catcctgacc ttcttcaatt tggcgcacta acctcaggaa
    82081 gcgatgctga gttttgttcc agaagtcgat atccacaaaa ctgaaatcac cccttgcacc
    82141 ttttggatcg gagaaccatg cttctttcaa tgcactccgt aacagactca gaatccgtga
    82201 agccgtttgc gcagccagcc gcagcttcgg tatctggcct tcttttttat tgagcagcag
    82261 cgggaaatgg tgttcgtacc aacagcgcgc tttcatgttg tcgaaatcat aaccaaatcc
    82321 ccacaggccc acttttgcct gtttcagact gctggcatta aagagtttca ccaccagcgc
    82381 gggaagttcc gtattgtttt ctgacttacc cgtttcgata aggcctaacc agtcgcgcca
    82441 gattaaaccg cccggttgtg gtttaacgga gtaaaactca ccgccctctt taagtggtac
    82501 acggtaaggc gttaagggat gctgccacat ggcataattc gcaccgtaat ttttggtagt
    82561 catcaaactc agaagcgcgt cactctgctc accgcaaata tcgcagttgc cgactgtcgt
    82621 ggtattaaaa tcaatacgaa tacgccgcgg cattccccag tacgcctgga gtttattgac
    82681 ctgatcatcg gttaccaccg caccggccag ttcgctggta cgcgtcgggc caagccaggg
    82741 gaaaaccaga tcgtcaaatt ttttgggtag cggtaagtcg gcttcatcct gcggcatcac
    82801 gttgagccac agtttgcgcc acaagggggc ttgttgattg ccctgatact cctgcaattc
    82861 aatcagagtc gtcatcggcc caccgccgcg taaaccggtg cgatagcctt tgccacctga
    82921 cggcgcattt aactgtaggg agaacagagc taacgcagaa caatgagagc atacgtgttc
    82981 agtcacgcca cgcttaataa agtggtcttt attaaacttc gttgtttgag cgccgggaat
    83041 ctcaggcagt agcgaagcga cctgaacttt atcgcccatg agcacctcga aatcctgcat
    83101 aaatgaaggt gaatctgggc caaactggaa agggtgttct aatgacagca atgcttcccg
    83161 tagcttttca gcttccagcc cgtcttccca gatatcatcc caacgacgat aatcttttgg
    83221 cgcgaaactg ctttgtagta accccagcaa aaactgccat gccgccccct ggagatctgc
    83281 ccgcggcgca gcgatatcga caacattttc atccgccaga tcgactggcg ccagcttgcc
    83341 tgttgttccg tctttaaaac gaacgggcaa ccacggggtt gtcagaagtg aaaacgagtt
    83401 catatgtatc ctctcgtaaa aaagaccatc cctggtcggt tgtccatcat tccatccttt
    83461 tcggtgtatc aactattcag agagtcctgt cgcaggtatc tcaatcaacc ttgccaatca
    83521 atccctccct ggccgaatac ccgcaacttt cgtcatcagt gactaaaatc acgtttgcca
    83581 tttccggatc ttgccgctgt tcaatgcacc actgcctgaa cgcttcccct tccagtaaag
    83641 aaaactcatc ccgatgtttt ttccaccagc ttcgacgcac tctgacaacg ctcatttccc
    83701 atgcgtgagc accggtggca taaggcttca ccacaccggc aatacaggta gccagccaca
    83761 gggaaacaga ttcctcagcc agacgtgtcg acagcttttc cggaaggtaa tcgttgatat
    83821 tggcggcata gccaggcttg aagttcagga caaacttttt agccattgcg cgatcgcagt
    83881 aatatttgcc cacttgctcc tgctcgctgc gggcaaatcc ttccggcatt accacgtcct
    83941 caccgtagac tgattcaata agaaggcggg ctgcgtgtgg catttgaata gcgccttgct
    84001 cacgcagtac acgctgcgtc agccagattc gtccatgatc gggatagaca tatgcactgt
    84061 tacgcatggc actgccgaac cattcgtcac caggagcgtc gtcccagacg ggggccagaa
    84121 tcagcaattc aggaggggaa cgctcgtctt ttccgtcacg ctttaactga ccattaatat
    84181 cgcggatatg ccgctgtaat cgccccgctc gctgaatcag caaatcaaca ggggccaggt
    84241 cggagatcat ttcgtccagg tcacaatcaa cgctctgctc taagacctga gtacaaatga
    84301 ggacttttcc ggcacgctgt gaaccgtctt ctttaccaaa gcgtgccagc gtctccattt
    84361 caattcgctg gcgatcgcta aaagcaaagc ggctatgaaa gagtgaaagg ctggaagcgg
    84421 gaatgacgcc gcgggcaagc agctgacgat gaaccttaat agcgtcatcg acagaattcc
    84481 ggatccaggc gatgcatttt ccctgactta ccgccgattc gatacgcgca atactctctt
    84541 gttcactatg aagccaaccc acgctgacgc tacgctcaac gtctttgcgc gtcgctaccc
    84601 ggtgtgagtt cacatcggat ttcgtgacat gcgtcagcca ggggtaatca tccttttcaa
    84661 ggaacggagc ttcttgctgg ccctctgtgc cacgcgcaaa ggcggcgacg agtttgtcgc
    84721 gctgctgttg ggataacgta gcagaaagca aaatgacgct gtttccgcca cgcgcctgcc
    84781 gctcgatcag cccttcaaga atgcacgaca tgtaagcatc acaggcatgg atctcatcag
    84841 ccagcaggat tttgttactc aaccccagaa gccgcagatt attatgttta aacggcatca
    84901 ctgccatcat cgcctgatcc agcgtgccga cgccaatttc agccagtagc gccttcttgt
    84961 tactgttggc aaaccaggcc gcacatccct gactgaatgt ttgttcatcc ggttcttctg
    85021 acccgactaa atcaccggac cagagtgatt cattgaagcg gtccattaat gtgcgggcac
    85081 tgtgtgccag caccaagctg gggcgggact ctggcgaata gaaagcaagc caggttttga
    85141 ccagccgatc gtacatggca ttggccgttg ccattgttgg caggccaaaa aacaaaccct
    85201 gtgctttcct cgcagccatc aacctgtgcg ccaggataag cgccgcttct gttttacctg
    85261 cgccagtcac gtcttccaga ataaataact gtggccctgg ctggctgata tccagatcca
    85321 gtaccttttg ctgtaatggt gtcgggtgct caataaaagg aaacagcgta ttaattccgg
    85381 tgaaaggtgc ggtttctgct tttggaggaa agacggttaa ggcgttttga gcctgaacta
    85441 aagttttctg ccagtaatct ttaatatcca ttgggtgtgc gacgcgtgga aaaaatcgcg
    85501 ttgacgaacc cgtccagtct gcgagtacga ctgttgcaga gatataccag gaaagttgtt
    85561 ttaaaagttc aacgccctcg tcatcatccc agaatgtggg aatctctatg agcggaaaca
    85621 gtgccttgat ttcaaggaga aaatctcgcg cggcagcttt gtcttcaggc agaaaattat
    85681 ccagctcatc aatacggtca ggtggtcgac catgatgccc ggtagttatg gacatccaca
    85741 tctctattac acgtgtaagt ttacgagaag agagtgaaga tgaaggaagc aactcctcac
    85801 attcacttaa ataataattc cacagccagt aacccagcgt tgaatgagag atcttttcgt
    85861 aattctttct ggaaccttcc ggaatcttga gttcaggggc caggtaaagt tgctgaaaag
    85921 agcgggcaaa ttttccaata tcgtgccagc acagcaacca agcgaaaaat tgagccgcct
    85981 gttccttgtc agaaatccct aattgacgaa agtaatcagc cagcccgaag caatttcttt
    86041 taaccattaa atagcccatt gcggccacat ccagcgaatg ccagcaaaga aggtgatagc
    86101 cgtcgccacc ctctttctcg ccacgtcggg tttttcccca gaaatcaaag
//
LOCUS       DAZKIF010000004        86044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZKIF010000004 DAZKIF010000000
VERSION     DAZKIF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.004"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..413
                     /locus_tag="ACHOTW_000285"
     CDS             <1..413
                     /locus_tag="ACHOTW_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9842464.1"
                     /translation="DVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKN
                     SLQMRIDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSG
                     HGTVNMRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(456..1316)
                     /gene="rsmI"
                     /locus_tag="ACHOTW_000286"
     CDS             complement(456..1316)
                     /gene="rsmI"
                     /locus_tag="ACHOTW_000286"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9842465.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1381..3417
                     /gene="lpoA"
                     /locus_tag="ACHOTW_000287"
     CDS             1381..3417
                     /gene="lpoA"
                     /locus_tag="ACHOTW_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP9842466.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3375..3770
                     /gene="yraN"
                     /locus_tag="ACHOTW_000288"
     CDS             3375..3770
                     /gene="yraN"
                     /locus_tag="ACHOTW_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP9842467.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            3790..4380
                     /gene="diaA"
                     /locus_tag="ACHOTW_000289"
     CDS             3790..4380
                     /gene="diaA"
                     /locus_tag="ACHOTW_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP9842468.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4390..4965
                     /gene="dolP"
                     /locus_tag="ACHOTW_000290"
     CDS             4390..4965
                     /gene="dolP"
                     /locus_tag="ACHOTW_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP9842469.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5079..6119)
                     /gene="yraQ"
                     /locus_tag="ACHOTW_000291"
     CDS             complement(5079..6119)
                     /gene="yraQ"
                     /locus_tag="ACHOTW_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9842470.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6192..6827)
                     /gene="yraR"
                     /locus_tag="ACHOTW_000292"
     CDS             complement(6192..6827)
                     /gene="yraR"
                     /locus_tag="ACHOTW_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP9842471.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6955..7473
                     /gene="yhbO"
                     /locus_tag="ACHOTW_000293"
     CDS             6955..7473
                     /gene="yhbO"
                     /locus_tag="ACHOTW_000293"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP9842472.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7453..7896)
                     /gene="yhbP"
                     /locus_tag="ACHOTW_000294"
     CDS             complement(7453..7896)
                     /gene="yhbP"
                     /locus_tag="ACHOTW_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP9842473.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            7947..8249
                     /gene="yhbQ"
                     /locus_tag="ACHOTW_000295"
     CDS             7947..8249
                     /gene="yhbQ"
                     /locus_tag="ACHOTW_000295"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP9842474.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(8236..8739)
                     /gene="yhbS"
                     /locus_tag="ACHOTW_000296"
     CDS             complement(8236..8739)
                     /gene="yhbS"
                     /locus_tag="ACHOTW_000296"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9842475.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8733..9257)
                     /gene="ubiT"
                     /locus_tag="ACHOTW_000297"
     CDS             complement(8733..9257)
                     /gene="ubiT"
                     /locus_tag="ACHOTW_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP9842476.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9466..10461
                     /gene="ubiU"
                     /locus_tag="ACHOTW_000298"
     CDS             9466..10461
                     /gene="ubiU"
                     /locus_tag="ACHOTW_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9842477.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10470..11348
                     /gene="ubiV"
                     /locus_tag="ACHOTW_000299"
     CDS             10470..11348
                     /gene="ubiV"
                     /locus_tag="ACHOTW_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9842478.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11429..12436
                     /gene="yhbW"
                     /locus_tag="ACHOTW_000300"
     CDS             11429..12436
                     /gene="yhbW"
                     /locus_tag="ACHOTW_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFP9842479.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKARHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12553..13797)
                     /gene="mtr"
                     /locus_tag="ACHOTW_000301"
     CDS             complement(12553..13797)
                     /gene="mtr"
                     /locus_tag="ACHOTW_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP9842480.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13951..15840)
                     /gene="deaD"
                     /locus_tag="ACHOTW_000302"
     CDS             complement(13951..15840)
                     /gene="deaD"
                     /locus_tag="ACHOTW_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP9842481.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15833..15913)
                     /gene="yrbN"
                     /locus_tag="ACHOTW_000303"
     CDS             complement(15833..15913)
                     /gene="yrbN"
                     /locus_tag="ACHOTW_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP9842482.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16020..16904)
                     /gene="nlpI"
                     /locus_tag="ACHOTW_000304"
     CDS             complement(16020..16904)
                     /gene="nlpI"
                     /locus_tag="ACHOTW_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP9842483.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17013..19148)
                     /gene="pnp"
                     /locus_tag="ACHOTW_000305"
     CDS             complement(17013..19148)
                     /gene="pnp"
                     /locus_tag="ACHOTW_000305"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9842484.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19395..19664)
                     /gene="rpsO"
                     /locus_tag="ACHOTW_000306"
     CDS             complement(19395..19664)
                     /gene="rpsO"
                     /locus_tag="ACHOTW_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9842485.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(19813..20757)
                     /gene="truB"
                     /locus_tag="ACHOTW_000307"
     CDS             complement(19813..20757)
                     /gene="truB"
                     /locus_tag="ACHOTW_000307"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9842486.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20757..21158)
                     /gene="rbfA"
                     /locus_tag="ACHOTW_000308"
     CDS             complement(20757..21158)
                     /gene="rbfA"
                     /locus_tag="ACHOTW_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9842487.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21323..23995)
                     /gene="infB"
                     /locus_tag="ACHOTW_000309"
     CDS             complement(21323..23995)
                     /gene="infB"
                     /locus_tag="ACHOTW_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9842488.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24020..25507)
                     /gene="nusA"
                     /locus_tag="ACHOTW_000310"
     CDS             complement(24020..25507)
                     /gene="nusA"
                     /locus_tag="ACHOTW_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9842489.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25535..25987)
                     /gene="rimP"
                     /locus_tag="ACHOTW_000311"
     CDS             complement(25535..25987)
                     /gene="rimP"
                     /locus_tag="ACHOTW_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9842490.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26194..26270)
                     /locus_tag="ACHOTW_000312"
     tRNA            complement(26194..26270)
                     /locus_tag="ACHOTW_000312"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26234..26236),aa:Met,seq:cat)
     gene            26618..27961
                     /gene="argG"
                     /locus_tag="ACHOTW_000313"
     CDS             26618..27961
                     /gene="argG"
                     /locus_tag="ACHOTW_000313"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP9842491.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(27969..29594)
                     /gene="yhbX"
                     /locus_tag="ACHOTW_000314"
     CDS             complement(27969..29594)
                     /gene="yhbX"
                     /locus_tag="ACHOTW_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9842492.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(30053..30139)
                     /locus_tag="ACHOTW_000315"
     tRNA            complement(30053..30139)
                     /locus_tag="ACHOTW_000315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30103..30105),aa:Leu,seq:gag)
     gene            complement(30154..30486)
                     /gene="secG"
                     /locus_tag="ACHOTW_000316"
     CDS             complement(30154..30486)
                     /gene="secG"
                     /locus_tag="ACHOTW_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9842493.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30714..32051)
                     /gene="glmM"
                     /locus_tag="ACHOTW_000317"
     CDS             complement(30714..32051)
                     /gene="glmM"
                     /locus_tag="ACHOTW_000317"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9842494.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32044..32892)
                     /gene="folP"
                     /locus_tag="ACHOTW_000318"
     CDS             complement(32044..32892)
                     /gene="folP"
                     /locus_tag="ACHOTW_000318"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP9842495.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32982..34916)
                     /gene="ftsH"
                     /locus_tag="ACHOTW_000319"
     CDS             complement(32982..34916)
                     /gene="ftsH"
                     /locus_tag="ACHOTW_000319"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9842496.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35016..35645)
                     /gene="rlmE"
                     /locus_tag="ACHOTW_000320"
     CDS             complement(35016..35645)
                     /gene="rlmE"
                     /locus_tag="ACHOTW_000320"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP9842497.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35771..36064
                     /gene="yhbY"
                     /locus_tag="ACHOTW_000321"
     CDS             35771..36064
                     /gene="yhbY"
                     /locus_tag="ACHOTW_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9842498.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36220..36696)
                     /gene="greA"
                     /locus_tag="ACHOTW_000322"
     CDS             complement(36220..36696)
                     /gene="greA"
                     /locus_tag="ACHOTW_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9842499.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36944..38377
                     /gene="dacB"
                     /locus_tag="ACHOTW_000323"
     CDS             36944..38377
                     /gene="dacB"
                     /locus_tag="ACHOTW_000323"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9842500.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38417..39589)
                     /gene="cgtA"
                     /locus_tag="ACHOTW_000324"
     CDS             complement(38417..39589)
                     /gene="cgtA"
                     /locus_tag="ACHOTW_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP9842501.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39605..40570)
                     /gene="yhbE"
                     /locus_tag="ACHOTW_000325"
     CDS             complement(39605..40570)
                     /gene="yhbE"
                     /locus_tag="ACHOTW_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9842502.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40697..40954)
                     /gene="rpmA"
                     /locus_tag="ACHOTW_000326"
     CDS             complement(40697..40954)
                     /gene="rpmA"
                     /locus_tag="ACHOTW_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9842503.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40975..41286)
                     /gene="rplU"
                     /locus_tag="ACHOTW_000327"
     CDS             complement(40975..41286)
                     /gene="rplU"
                     /locus_tag="ACHOTW_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9842504.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41545..42516
                     /gene="ispB"
                     /locus_tag="ACHOTW_000328"
     CDS             41545..42516
                     /gene="ispB"
                     /locus_tag="ACHOTW_000328"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP9842505.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42745..43023
                     /gene="sfsB"
                     /locus_tag="ACHOTW_000329"
     CDS             42745..43023
                     /gene="sfsB"
                     /locus_tag="ACHOTW_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP9842506.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43071..44330)
                     /gene="murA"
                     /locus_tag="ACHOTW_000330"
     CDS             complement(43071..44330)
                     /gene="murA"
                     /locus_tag="ACHOTW_000330"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9842507.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44385..44639)
                     /gene="ibaG"
                     /locus_tag="ACHOTW_000331"
     CDS             complement(44385..44639)
                     /gene="ibaG"
                     /locus_tag="ACHOTW_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP9842508.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44799..45092)
                     /gene="mlaB"
                     /locus_tag="ACHOTW_000332"
     CDS             complement(44799..45092)
                     /gene="mlaB"
                     /locus_tag="ACHOTW_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP9842509.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45092..45727)
                     /gene="mlaC"
                     /locus_tag="ACHOTW_000333"
     CDS             complement(45092..45727)
                     /gene="mlaC"
                     /locus_tag="ACHOTW_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP9842510.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45746..46297)
                     /gene="mlaD"
                     /locus_tag="ACHOTW_000334"
     CDS             complement(45746..46297)
                     /gene="mlaD"
                     /locus_tag="ACHOTW_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP9842511.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46302..47084)
                     /gene="mlaE"
                     /locus_tag="ACHOTW_000335"
     CDS             complement(46302..47084)
                     /gene="mlaE"
                     /locus_tag="ACHOTW_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP9842512.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47092..47901)
                     /gene="mlaF"
                     /locus_tag="ACHOTW_000336"
     CDS             complement(47092..47901)
                     /gene="mlaF"
                     /locus_tag="ACHOTW_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP9842513.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48111..49088
                     /gene="yrbG"
                     /locus_tag="ACHOTW_000337"
     CDS             48111..49088
                     /gene="yrbG"
                     /locus_tag="ACHOTW_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP9842514.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49102..50088
                     /gene="kdsD"
                     /locus_tag="ACHOTW_000338"
     CDS             49102..50088
                     /gene="kdsD"
                     /locus_tag="ACHOTW_000338"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP9842515.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50109..50675
                     /gene="kdsC"
                     /locus_tag="ACHOTW_000339"
     CDS             50109..50675
                     /gene="kdsC"
                     /locus_tag="ACHOTW_000339"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP9842516.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50672..51247
                     /gene="lptC"
                     /locus_tag="ACHOTW_000340"
     CDS             50672..51247
                     /gene="lptC"
                     /locus_tag="ACHOTW_000340"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP9842517.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51216..51773
                     /gene="lptA"
                     /locus_tag="ACHOTW_000341"
     CDS             51216..51773
                     /gene="lptA"
                     /locus_tag="ACHOTW_000341"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP9842518.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51780..52505
                     /gene="lptB"
                     /locus_tag="ACHOTW_000342"
     CDS             51780..52505
                     /gene="lptB"
                     /locus_tag="ACHOTW_000342"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP9842519.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52553..53986
                     /gene="rpoN"
                     /locus_tag="ACHOTW_000343"
     CDS             52553..53986
                     /gene="rpoN"
                     /locus_tag="ACHOTW_000343"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9842520.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54009..54296
                     /gene="hpf"
                     /locus_tag="ACHOTW_000344"
     CDS             54009..54296
                     /gene="hpf"
                     /locus_tag="ACHOTW_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP9842521.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54414..54905
                     /gene="ptsN"
                     /locus_tag="ACHOTW_000345"
     CDS             54414..54905
                     /gene="ptsN"
                     /locus_tag="ACHOTW_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP9842522.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            54951..55805
                     /gene="rapZ"
                     /locus_tag="ACHOTW_000346"
     CDS             54951..55805
                     /gene="rapZ"
                     /locus_tag="ACHOTW_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9842523.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55802..56074
                     /gene="npr"
                     /locus_tag="ACHOTW_000347"
     CDS             55802..56074
                     /gene="npr"
                     /locus_tag="ACHOTW_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP9842524.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56288..56920
                     /gene="yrbL"
                     /locus_tag="ACHOTW_000348"
     CDS             56288..56920
                     /gene="yrbL"
                     /locus_tag="ACHOTW_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP9842525.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(56917..57645)
                     /gene="mtgA"
                     /locus_tag="ACHOTW_000349"
     CDS             complement(56917..57645)
                     /gene="mtgA"
                     /locus_tag="ACHOTW_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP9842526.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57642..58295)
                     /gene="elbB"
                     /locus_tag="ACHOTW_000350"
     CDS             complement(57642..58295)
                     /gene="elbB"
                     /locus_tag="ACHOTW_000350"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP9842527.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58525..60861)
                     /gene="arcB"
                     /locus_tag="ACHOTW_000351"
     CDS             complement(58525..60861)
                     /gene="arcB"
                     /locus_tag="ACHOTW_000351"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP9842528.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60957..61886)
                     /gene="yhcC"
                     /locus_tag="ACHOTW_000352"
     CDS             complement(60957..61886)
                     /gene="yhcC"
                     /locus_tag="ACHOTW_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9842529.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62560..67020
                     /gene="gltB"
                     /locus_tag="ACHOTW_000353"
     CDS             62560..67020
                     /gene="gltB"
                     /locus_tag="ACHOTW_000353"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP9842530.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67033..68451
                     /gene="gltD"
                     /locus_tag="ACHOTW_000354"
     CDS             67033..68451
                     /gene="gltD"
                     /locus_tag="ACHOTW_000354"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP9842531.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68635..69684
                     /gene="yhcG"
                     /locus_tag="ACHOTW_000355"
     CDS             68635..69684
                     /gene="yhcG"
                     /locus_tag="ACHOTW_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFP9842532.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLPWSTYVRLLSVKNADTRSFYEKETLRCGWSVRQLERQIATQFYERTL
                     LSHDKSAMLQQHAPAETHILPQQAIRDPFVLEFLELKDEYSESDFEEALINHLMDFML
                     ELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLLIVDLKVGKFSYSDAGQMNMYL
                     NYAKEHWTLPDENPPIGLVLCAEKGVGEAHYALAGLPNTVLASEYKMQLPDEKRLADE
                     LVRTQAVLEEGYRLR"
     gene            complement(69744..70208)
                     /gene="nanQ"
                     /locus_tag="ACHOTW_000356"
     CDS             complement(69744..70208)
                     /gene="nanQ"
                     /locus_tag="ACHOTW_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP9842533.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(70205..71080)
                     /gene="nanK"
                     /locus_tag="ACHOTW_000357"
     CDS             complement(70205..71080)
                     /gene="nanK"
                     /locus_tag="ACHOTW_000357"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP9842534.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(71077..71766)
                     /gene="nanE"
                     /locus_tag="ACHOTW_000358"
     CDS             complement(71077..71766)
                     /gene="nanE"
                     /locus_tag="ACHOTW_000358"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9842535.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(71814..73304)
                     /gene="nanT"
                     /locus_tag="ACHOTW_000359"
     CDS             complement(71814..73304)
                     /gene="nanT"
                     /locus_tag="ACHOTW_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP9842536.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(73413..74306)
                     /gene="nanA"
                     /locus_tag="ACHOTW_000360"
     CDS             complement(73413..74306)
                     /gene="nanA"
                     /locus_tag="ACHOTW_000360"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP9842537.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(74428..75219)
                     /gene="nanR"
                     /locus_tag="ACHOTW_000361"
     CDS             complement(74428..75219)
                     /gene="nanR"
                     /locus_tag="ACHOTW_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP9842538.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            75599..76955
                     /gene="dcuC"
                     /locus_tag="ACHOTW_000362"
                     /pseudo
     CDS             75599..76955
                     /gene="dcuC"
                     /locus_tag="ACHOTW_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(76998..77495)
                     /gene="sspB"
                     /locus_tag="ACHOTW_000363"
     CDS             complement(76998..77495)
                     /gene="sspB"
                     /locus_tag="ACHOTW_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP9842539.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77501..78139)
                     /gene="sspA"
                     /locus_tag="ACHOTW_000364"
     CDS             complement(77501..78139)
                     /gene="sspA"
                     /locus_tag="ACHOTW_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP9842540.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78534..78926)
                     /gene="rpsI"
                     /locus_tag="ACHOTW_000365"
     CDS             complement(78534..78926)
                     /gene="rpsI"
                     /locus_tag="ACHOTW_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9842541.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78942..79370)
                     /gene="rplM"
                     /locus_tag="ACHOTW_000366"
     CDS             complement(78942..79370)
                     /gene="rplM"
                     /locus_tag="ACHOTW_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9842542.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79589..80716)
                     /gene="zapE"
                     /locus_tag="ACHOTW_000367"
     CDS             complement(79589..80716)
                     /gene="zapE"
                     /locus_tag="ACHOTW_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP9842543.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80910..81308
                     /gene="zapG"
                     /locus_tag="ACHOTW_000368"
     CDS             80910..81308
                     /gene="zapG"
                     /locus_tag="ACHOTW_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP9842544.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81462..82829
                     /gene="degQ"
                     /locus_tag="ACHOTW_000369"
     CDS             81462..82829
                     /gene="degQ"
                     /locus_tag="ACHOTW_000369"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP9842545.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            82919..83986
                     /gene="degS"
                     /locus_tag="ACHOTW_000370"
     CDS             82919..83986
                     /gene="degS"
                     /locus_tag="ACHOTW_000370"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP9842546.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(84050..84988)
                     /gene="mdh"
                     /locus_tag="ACHOTW_000371"
     CDS             complement(84050..84988)
                     /gene="mdh"
                     /locus_tag="ACHOTW_000371"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9842547.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            85423..85893
                     /gene="argR"
                     /locus_tag="ACHOTW_000372"
     CDS             85423..85893
                     /gene="argR"
                     /locus_tag="ACHOTW_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP9842548.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        21049 a        23235 c        21440 g        20320 t
ORIGIN      
        1 gcgatgttat taaggttaat tttggattca tcaatggtca gaagtttacc acccgcaatg
       61 ccatgccaga cggttttact ccagtagact ttgatatcac ttatgactgt ggtgatactt
      121 caaagattaa aaactcgttg caaatgcgca tcgacggtac aactggggta gtagaccagt
      181 acaacctggt cgccagacga agaagttcag acaatgcgcc cgatgtcggt attcgtattg
      241 aaaatctcgg cggcggagtt gcaaatattc cttttcagaa cggtatcctt cccgttgatc
      301 cttccgggca tggcaccgtc aatatgcgcg cctggccagt taatctggtc ggtggtgagc
      361 tggaaacagg aaaatttcag ggcacagcca ccattaccgt catcgtgcgg taaaagaaga
      421 cgttgcgttt tgattaacgc aacgcttcgg cactcttacc cctgctgctc cagcgcatac
      481 ttatacagcg catttttctt cacgccgtga atttctgcgg ccagcgccgc cgcttttttc
      541 agcggcagtt ctgcctgtag cagcgccagc gtgcgcaggg catcggcggg taagtcttct
      601 tcctgtgctt tatgaccttc gacaatcagc accatttcgc ctttgcgacg gttttcatct
      661 tcctttaccc acgccagcag ctcgccaacg ggcgcgccgt gaatggtttc ccaggttttg
      721 gtcagctcac gcgccagaac cacgtagcgg gattcgccta ataccgcaac gatatcttcc
      781 aggctatcta acagacggtg ggtagattca taaaaaatca gcgtgcgcgg ctccgcttca
      841 atggctttta gcgcatcacg gcggcctttt gatttggcag gtaaaaagcc ttcgtaacag
      901 aaacggtcag agggtaaacc cgcggcgctt aacgcagtga tagcagcaca cggtccgggt
      961 agcggcacca cacgaatccc cgcttcacgg caggtacgca ccagatggta gccaggatcg
     1021 ttaattagcg gcgttccggc atcggaaacc agcgcaatgt tttgcccctc ttgcagcttc
     1081 gccagcagcg tttcggcttt ttgttgttcg ttatggtcgt gcagcgcaaa caaccgggca
     1141 ttaatcccaa aatgttgcag caataaaccg gtgtgacgag tatcctcggc ggcaatcaga
     1201 tcaacggcct gtaatacctc taacgcacgc tgggtgatat ccgccagatt gccgattggc
     1261 gtcggtacaa tgtaaagctg gccctgagaa ttatccgccg attggtgttg tttcattgtg
     1321 tcgtccgtat tgccgattta atattgagca ttgcgtaaaa aaaatatcac tggatacatt
     1381 atggtaccct caacattttc tcgtttgaaa gccgcgcgtt gtctgcctgt tgttctggca
     1441 gccctgattt tcgtcggttg tggcacccat actcccgatc agtccactgc ttatatgcag
     1501 ggcacggcgc aggctgattc tgccttttat cttcagcaga tgcagcaaag ctctgatgat
     1561 accaggatca actggcaatt actcgccatt cgtgcactgg tgaaagaagg taaaaccggg
     1621 caggcggttg agttgtttaa ccaactaccg caagaactga acgattctca gcgtcgcgag
     1681 aaaacactgc tggcggtaga gattaaactg gcgcagaaag attttgctgg cgcgcaaaac
     1741 ttgctggcga aaattacacc cgccgattta gaacaaaacc agcaagcccg ttactggcag
     1801 gcaaaaatcg atgccagcca ggggcgtcct tccattgatt tactgcgcgc gttaattgct
     1861 caggaaccgc tgcttggcgc gaaagaaaaa cagcagaata ttgatgccac ctggcaggcg
     1921 ctctcctcca tgactcagga acaggcgaat acgctggtga tcaacgccga cgaaaatatt
     1981 ctgcaaggct ggctggatct gcagcgcgtc tggtttgata accgtaacga tcccgacatg
     2041 atgaaagccg ggatcgccga ctggcagaaa cgttatccga acaatccggg cgcgaaaatg
     2101 ctgccaacgc agttggttaa cgtaaaagcg tttaaaccag cctcgaccaa caaaatcgcc
     2161 ctgctgttgc cactgaatgg ccaggcagcg gtatttggtc gcactattca gcaaggcttt
     2221 gaagcggcga aaaatatcgg cactcagcca gtggcggctc aggtagctgc cgcacctgcc
     2281 gcagacgtag cagaacaacc tcagccgcaa actgcggatg gcgttgccag cccggcacaa
     2341 gcctcggtta gcgatctgac cggtgatcag cctgcggccc agccggtgcc tgtaagcgcc
     2401 ccggcgacaa gcaccgcagc ggtaagcgca cccgcaaatc catccgcaga gctgaaaatc
     2461 tacgatacct catcacaacc acttagccag atcttaagcc aggttcagca ggatggcgcg
     2521 agtattgtgg ttggtccgtt gctgaaaaat aacgttgaag agttgctgaa gagcaacact
     2581 ccgctgaacg tactagcact gaaccagccg gagaatatcg aaaatcgcgt caatatttgt
     2641 tacttcgcgc tttcaccgga agacgaagcg cgcgatgcag cgcgtcatat tcgtgaccag
     2701 ggtaaacaag cgccgctggt gctgatccca cgcagttcat tgggcgatcg cgtagccaat
     2761 gcgtttgcgc aagagtggca gaaattgggc ggcggcaccg ttctgcaaca aaaatttggt
     2821 tccaccagcg aattacgcgc gggtgttaac ggcggttctg gtattgcttt aacgggtacc
     2881 ccgattactc ccagagcgac aaccgactcc ggcatgacga ccaacaatcc aacgctgcaa
     2941 accacgccaa ccgatgacca gttcaccaat aatggcggtc gtgtcgatgc ggtgtacatt
     3001 gtggcaacgc cgggtgaaat cgcttttatt aaaccgatga tcgccatgcg taacggtagc
     3061 cagagcggtg caacgctgta cgccagctcc cgcagtgcgc aagggaccgc tggcccggat
     3121 ttccgtctgg agatggaagg cttgcagtac agcgaaatcc cgatgctggc gggcggtaat
     3181 ctgccgttaa tgcagcaggc actcagcgcg gtgaataacg attattcact ggctcgcatg
     3241 tatgcgatgg gcgtcgatgc ctggtcgctg gcaaatcatt tctcacaaat gcgccaggtt
     3301 cagggttttg aaatcaacgg taataccgga agcctgacgg ctaacccgga ttgcgtgatt
     3361 aacaggaagt tatcatggct acagtaccaa caaggtcagg tagtccccgc cagttaacca
     3421 ccaaacagac cggcgatgcg tgggaagtac aagcgcgtcg ctggctggaa ggcaaaggac
     3481 tgcgatttgt cgccgctaac gtgaacgagc gtggcggcga gatcgatctg ataatgcgtg
     3541 aaggccggac caccgttttt gtcgaggtgc gttaccgccg ttctgcgctt tatggcggcg
     3601 cggcagccag tgtgacccgc agcaaacaac acaaattatt acagactgcc cgcttgtggc
     3661 tcgcgcgtca taatgggagt tttgatactg tggattgccg gttcgatgtg gtagccttca
     3721 ccgggaatga ggttgagtgg attaaggata cctttaatga ccactcataa ttaaggttta
     3781 aggattagcg tgcaagaaag aattaaagct tgcttcactg aaagcattca aactcaaatt
     3841 gcggcggcag aggcgcttcc ggatgccatc tcccgtgcgg ccatgacgct ggttcagtct
     3901 ctgctcaatg gcaacaaaat cctctgttgt ggtaatggaa cttccgctgc caatgcacag
     3961 cattttgctg ccagcatgat caaccgtttc gaaacggagc ggcccagctt acctgccatt
     4021 gcactaaata ccgataatgt tgtcttaacg gcgattgcca acgatcgctt acatgatgaa
     4081 gtgtatgcaa aacaggtgcg ggcgctgggg catgcgggag atgtattgtt agccatttcc
     4141 acccgtggca acagccgcga tatcgtaaaa gcagtagaag ctgccgttac gcgtgatatg
     4201 accattgtgg cattgaccgg ttatgacggc ggtgaacttg caggtttgtt agggccacag
     4261 gatgtggaga tccgcatccc ttcgcatcgc agtgctcgca ttcaggaaat gcatatgctg
     4321 acggtaaatt gcctgtgcga tctgatcgat aacacgcttt tccctcacca ggatgattaa
     4381 ggagaataca tgaaggcatt atcgccaatc gcagtcctta tttccgcgct gctgttgcaa
     4441 ggttgtgttg ccgctgccgt agtgggtact gctgctgtgg gtaccaaagc cgcaactgac
     4501 ccacgcagtg tcggcaccca ggtggacgat ggtaccctgg aagtgcgcgt gaacagcgca
     4561 ttgtcgaaag acgaacagat taagaaagaa gcgcgcatta atgtaacggc ctatcagggc
     4621 aaagtgctgc tggttgggca gtcaccaaac gctgaacttt cggctcgcgc caaacagatt
     4681 gctatgggcg tagacggtgc caacgaagtg tataacgaga ttcgtcaggg ccagccgatt
     4741 ggtctgggcg aagcgtctaa cgatacgtgg atcaccacca aagtgcgttc gcagctctta
     4801 accagcgacc tggtgaaatc gtccaacgtg aaagtgacca ccgaaaacgg tgaagtgttc
     4861 ctgatggggc tggtgactga acgtgaagcg aaagcggcgg cagatattgc cagccgggtg
     4921 agcggcgtga agcgggtaac tacggcgttt acgtttatta aatagcagcc ccttgtaatg
     4981 cctgatgcga cgcttgccgc gtcttatcag gcctacaacg atgcaaaccg taggtcggat
     5041 aaggcgttta cgccgcatcc gacatgtttt ccctcaaatc agaataacag cgccagcccg
     5101 ccgacaatca cgcctgacac tgccaccatc gcccccgtca gccataaggc tttcgccggg
     5161 aacgctttgc gcagcataat cagtgacggc aaactcaccg ccgggagcgt catcaacaat
     5221 gccagcgccg gagcagttcc catacctgcc agcatcatcg tttgtacaat cggaatttct
     5281 gctgccgtgg gaatgacaaa caagcaccct gctaccgcca tcgccaccac ccacatcagg
     5341 ctgttatcga cagcaccatc ggcatggggg aataaccaga cgcgagcggc acccaacacc
     5401 agtactgcaa ggatgtaaac cgggatcgta ctccagaaaa gcgtccatag cgccctgccc
     5461 cagcgactaa aaaatccgcc ctgtgcttcc ggtatgtcaa tttcgaccgg tgcttgcgtt
     5521 tgcggtgttt cacgcaccca tttttgcacc agcgtcgcaa tcagcaacac catcaccagc
     5581 ccggccacca gacgaatcgc cgcaaaaccc cagccgagga caaaccccat aaacaccagc
     5641 gtcgccgggt ttaacaccgg attgcccatc cagaatgcca gcgcaccgcc catcgacacc
     5701 tgttggcgac gcattcccgc cgcgaccgga gccgcacagc aggtacacat catgcctggc
     5761 agcgaaaaca gcgttcccag cagcgtgccg cgaaagcgcg attgcccaag cgtacgcaac
     5821 aaccagtcac gcgggatcag cacctgaatc aacgaaccga ggatcacccc cagcaccgcc
     5881 gctttccata ccgcgaggaa atagatcatc gcgtaatcca acgccgcctg ccatggatta
     5941 gcatccgcct gcgcaaggat agatttaccg atactgtggg tttcggcagc agtaaacgct
     6001 ttgccgtagt aaggttgcca tttcacatac cagagaccgg caatgacaac gagaaagaaa
     6061 agcgcgggtt tccaccactg aatgggcgtt gccgcctgag atgaagactg accagtcata
     6121 gcattcccca ggaataagtt atgataattg agcgcgtgaa tattacgctc actatcaatt
     6181 cttgggaata attattcagc tcttttgcgt aattctgaag agcttaaaat cgtcacacct
     6241 tcatgctcgg gccgcatcgc ctctgccagc atgacccgcg ccacgtctct cgcatcaatg
     6301 gatttccagt taccgggtaa caaacggaac agcggcgcaa aaagcgtttc gttcatccgc
     6361 tgtttgctac gatcgcccag taacatcgac gggcgagcaa tggtcaattt cggccagttc
     6421 tgggcgatta atgcttcttc catctcccct ttgacgcggt tatagaaaaa cggcgagtgg
     6481 gcattggcac ccatagcact gaccaccagc atatgctgcg cgcccagtcg ccgcccggtt
     6541 aatgcggtat ccactaccag cgtgtaatcg gcatgaataa acgcctcttt gctccccgct
     6601 tctcgccgcg tggtgccgag acaacaaaac acaatgtcga tgggatcggt gacctgcgcc
     6661 agcgcgtcag tcagttgcgg atcatgggga ttaaataccc cgggcatatc gcccaacgga
     6721 cgtcgcgtcg gcgcggctat ggcgttaact ttcggttcgt taatcaacat ccgcagcagg
     6781 tgaccgccca ccagccccgt tgcgcctgta atcagtacct gactcatctt cgctccttta
     6841 cagaattgtc tgccttgcgc tccacggctc atgcaccagg cttaatagac catgaggtaa
     6901 ttatccccga ttgtgggaaa ttcgcctcat ccaatgcaac aacgcggagg aagcatgagt
     6961 aagaaaattg ccgttttaat cactgatgaa tttgaggatt cagaatttac ttcacccgca
     7021 gacgagttcc gtaaagccgg acacgaagtg attaccattg aaaaacaagc gggtaaaacg
     7081 gtgaaaggca aaaaaggaga agccagcgtg accatcgata aatccatcga tgaagtgacg
     7141 ccagcggagt ttgatgccct gttgctaccg ggcggtcatt caccggatta tctgcgtggc
     7201 gacaaccgtt ttgtcacctt tacccgtgat tttgtgaata gcggcaaacc tgttttcgcc
     7261 atctgtcacg gtccgcagct gctgatcagc gccgatgtga ttcgggggcg caaactgacc
     7321 gcggttaaac cgatcattat tgatgttaaa aacgcgggcg cggaatttta cgatcaggaa
     7381 gtcgtggtcg ataaagatca gctggttacc agccggacac cggacgatct gccagcgttt
     7441 aaccgcgagg cgttacgcct gctcggtgcc tgagccgcgt agccaaatca tttttttacc
     7501 aaagcccagc gtgttgtcgg tgaatttgat ttcatcgggc cggatttccc acaccggggc
     7561 cgacagcatt ctggcaaccg gaaagcgacg attgtacgcc ttgcgcgcga ggtcgctttc
     7621 ctcaccttcc agcctgcgga tctcaccttt aaactgcacg ccgcgaatta acgctaccgt
     7681 tttcggctga ccgtttaccg ttccggcaac cgcagcctgc ggcccactca tctgcgcgtg
     7741 gcgcgttttt tcttccgtca aaatgtagaa ggcgactttc tgcacatcaa acagataaaa
     7801 ggcattggcg caccagagtt ccccttcctg ctgcacacac caggtgacaa catgttgttt
     7861 tgccagccaa cggctgatgg cggtgagtgt ttccattgct gttctctttt atactgtggg
     7921 cctgacctta acatgcgacg cagacgatga caccctggtt tctttacttg atccgtaccg
     7981 ccgacaataa gctttatacc gggatcacca cggatgtcga acgccgctat cagcagcacc
     8041 aaagcggcaa aggggcgaaa gcactgcgcg ggaaaggaga actgacgctg gcgttttctg
     8101 cgccagtggg cgatcgttcg ctggcgttac gggcggaata tcgcgttaag caactgacca
     8161 aacggcaaaa agagcgtctg gtagcggaag gcgcagggtt tgcaaagctg ttaagcagtc
     8221 tgcaaacccc ggagattaaa agcgattgaa atgctcgtga tactcaacca gaccagttac
     8281 gccattcaac gcgtcatccg ctaaacgatg tacctggaag gcgctttcag tgcccggcca
     8341 gcggcaacgc agatcgtgat gcgccgccag ttcaaagccg aaacggctgt atagcgccgg
     8401 atcgcccagc gtcaccactg cggcatagcc gaactcatta agcgaatcga gtccttcata
     8461 gaccagttgg cgtgccagcc cctgtccacg gtatttttca tctaccgcca gcggtgccat
     8521 gccgacccat tgcaggtctt cgccctgcac atcaaccgga ctaaatgcca catagccaat
     8581 gacctgacct tcgtcatctg tcgccaccag ccccagcgtc agaaagccat cttcacgcag
     8641 atcgtgaacc agcttcgctt ccgcatcact ttcgaatgaa cgacgcagca gggcatcaat
     8701 acccggcgca tcaatgggaa tttctactcg aattagcatg gttcacctac cgatgtctgt
     8761 ttggtttcag gcgcggtttt cattcccgcc tcaacaaaat ccgccagttg cagcagcatc
     8821 atgcgtaagg ctttcggcat ttgctccagt tcaatggcgt ccatcaggtt tttcacatac
     8881 agccccagct ccgtatcgcc ttcaatcacc agccgacgct ggaagaagag cgtatccgga
     8941 tcttgtttac gcgccgcaat catcagcaga tcgctggcgt cggcactaaa actcacatca
     9001 gcttgcgcgt tctggctgac caccagtttg ccattcacca ccgaggtaaa ccattgcagg
     9061 tcaatatcac gcacatgaat acttaaccag cggccttcaa gaaactccag ctcaccatca
     9121 tccagcgcct ggcggaattg ccagcttaag acctgctcaa gaacctggcg ttttagcgca
     9181 aatggcgtca gttttaccgg tacactcaac agagatggcc ccaaatgcac aatacgggaa
     9241 cgcagtttat ccaacacgag ttttactccc tgtttcaaca atcatcctat tttgccatat
     9301 cagaaaaata acatagcggt ataaatcaac aattccatat gaaattgctg ctaccaccaa
     9361 tacaacttta actgccttaa atcaaaaatt gtcgcagcaa ggttaactaa aatcccagtt
     9421 cgttaacatt tttgcgtttt gatagcgcaa ccttcaggaa aaattatgga gctgctctgc
     9481 cctgccggaa atctcccggc gcttaaggcg gccatcgaaa acggcgcaga tgctgtttat
     9541 atcgggctaa aagatgatac caatgcccgt cacttcgccg gccttaactt taccgagaaa
     9601 aaattgcagg aagcggtgag ttttgtccat caacatcgcc gcaaacttca catcgcgatt
     9661 aacacttttg cgcatccgga cggttacgcc cgttggcagc gtgccgtgga tatggcggcg
     9721 cagctgggtg ccgacgcgct gatcctcgcc gacctcgcca tgctagagta cgccgccgag
     9781 cgttacccgc atattgagcg tcatgtgtca gtgcaggctt cggcgaccaa tgaagaggcg
     9841 attaactttt atcatcgcca ttttgacgtt gctcgcgtgg tgctgccgcg cgtgttgtcg
     9901 attcatcagg tgaaacaact ggcacgggtc acacctgtcc cgctggaagt ctttgctttc
     9961 ggcagcctgt gcattatgtc ggaaggtcgt tgctatctgt cgtcgtatct gacgggtgag
    10021 tcgcccaaca ccgtgggcgc gtgttctccg gcccgtttcg tgcgctggca gcaaacgccg
    10081 caggggctgg aatcccgcct gaacgaagtg ctgatcgacc gttatcagga cggcgaaaac
    10141 gcaggttatc cgacgctatg taaagggcgt tatctggtgg acggcgagcg ctatcacgcg
    10201 ctggaagaac caaccagtct caataccctg gaactgctgc cggagttaat ggcggcgaat
    10261 attgcttcgg tgaaaattga aggccgccaa cgtagcccgg cgtatgtcag ccaggtggcg
    10321 aaagtctggc gtcaggctat cgaccgttgt aaggccgatc cgcaaaactt cgtaccgcaa
    10381 agcgcgtgga tggagacgct cgggtcgatg tccgaaggca cgcagaccac ccttggcgcg
    10441 tatcaccgta aatggcagtg agaagagcaa tgaaatattc cttagggcca gtgctgtggt
    10501 actggccaaa agagacgctg gaagattttt atcagcaggc cgccaccagc agcgccgacg
    10561 tgatttatct tggcgaagcg gtttgcagca agcgccgggc aaccaaagtt ggcgactggc
    10621 tggagatggc aaaatcgctc gccggaagtg gtaagcagat tgtgctctcc acgctggcgc
    10681 tggtgcaggc atcatctgaa ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc
    10741 tgattgaagc cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg
    10801 tcgccggaca cgcgctgaac tgctataacg cggtgacgct gaaaatattg ctcaaacagg
    10861 gcatgatgcg ctggtgtatg ccggtggagc tttcccgcga ctggctggtg aatctgctta
    10921 atcagtgcga tgagctgggc attcgcaacc agtttgaagt tgaagtcctg agctacggtc
    10981 atctaccgct ggcctactcc gctcgctgct ttaccgcgcg ttcggaagac cgcccgaaag
    11041 atgagtgtga aacctgctgc attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa
    11101 accaacaagt gtttgtactc aatggcattc agaccatgag cggctacgtt tacaacctcg
    11161 gtaacgagct ggcatccatg cagggcctgg ttgatgtggt ccgcctgtca ccgcagggta
    11221 ctgacacttt cgcgatgctc gacgccttcc gcgctaatga aaatggcgct gcaccactgc
    11281 cgttgacggc gaacagcgac tgtaacggct actggcggcg gctggcagga ctggagctgc
    11341 aagcctaagt aaatagctca ctttgttaac aactttaact actctttaat gcagtattaa
    11401 agattaatcg gtaacaaagt gagctgttat gactgataaa accattgcgt tttcgctact
    11461 cgatctggcc cccattcccg aaggttcttc agcgcgagat gcattctccc actctctcga
    11521 tctcgcccgt ctggctgaaa agcgcggcta tcatcgctac tggctggcag aacaccacaa
    11581 tatgactggc attgccagtg ctgccacgtc ggtattgatt ggctatctgg cggcgaatac
    11641 caccacgctg catctggggt ctggcggcgt gatgttgcct aaccactcac cgttggtcat
    11701 tgcagaacag ttcggcacgc tcaatacact ctatccgggg cgaatcgatt tggggctggg
    11761 tcgtgctccg ggtagtgacc agcgaaccat gatggcgcta cgtcgtcata tgagcgggga
    11821 tattgataat ttcccccgcg atgtcgcgga gctggtggcc tggtttgacg cccgcgatcc
    11881 caatccgcat gtgcgcccgg taccaggcta cggcgagcaa atccccgtgt ggttgttagg
    11941 ctccagcctt tacagcgcgc aactggcggc gcagcttggt ctgccgtttg cgtttgcctc
    12001 acacttcgcg ccggatatgt tgttccaggc gctgcatctt tatcgcagca acttcaaacc
    12061 gtcggcacga ctggaaaaac catacgcgat ggtgtgcatc aatattatcg ctgccgacag
    12121 caaccgcgac gctgaatttc tgtttacctc aatgcagcaa gcctttgtga agctgcgccg
    12181 tggcgaaacc gggcaactgc cgccgccgat tcaaaatatg gatcagttct ggtcaccgtc
    12241 tgagcagtat ggcgtgcaac aggcgctgag tatgtcgctg gtgggcgata aagcgaaagc
    12301 gcgtcatggt ttgcagtcga tcctgcgcga aaccgacgcc gatgagatta tggttaacgg
    12361 gcagattttc gaccaccagg cgcgcctgca ttcgtttgag ctggcgatgg atgttaagga
    12421 agagttgttg ggatagtgtg tcttaacgcg ggaagcctta tccgagctgg caacgctgtc
    12481 ccacgtaggc ctgataagcg aagcgcatca ggcattgtgt aggcagcaga aatgtcggat
    12541 aaggcccgct gattactgat acaccggcag caaattaaag ctcgataaga tatggaccag
    12601 tgcgttgcca acgccaaata ccagaatcag cgtaatcatc ggcttgccgc cccagacgcg
    12661 gaatttcggg ctgccaaagc gtttacgcga tgcgcgggcc agcagtgccg gaacaattgc
    12721 cgcccagatg gtagctgcta aaccagcata accaatggcg tacaggaatc cgttcgggaa
    12781 cagcagacca cccacaacag gaggggcaaa ggtcagcaat gccgttttca agcggcccac
    12841 agccgagtcg tcgaaaccaa acagatctgc cagatagtca aacaaaccca gcgttacgcc
    12901 gaggaacgaa ctcgctaccg caaagtttga gaacacgacc agcagcagat ccagactacg
    12961 gctgttcagt acgccgctta acgcctgtac cagcacatca atattaccgc ccttctctgc
    13021 aatatcgata aactccggac gcgggatgtt acccatcgtc gccagcaacc agatggtata
    13081 cagcgccagc gccatcagcg taccgtacac cagacatttc acgatggttt tcggatcttt
    13141 gccgtaatac ttcatcaggc ttggcacgtt accgtgataa ccaaacgatg ccagacagaa
    13201 cggcaaggtc atcaacagat acggtgcata agacgcattg ctttcagcga cgttgaacaa
    13261 tgtcgcaggc tgcacatgcc ccagcaggct accgaaggtg aggaagaagg taatgacttt
    13321 cgcccccaat acaatcgctg tcatgcgact gacggcttta gtgctcaacc acaccacaaa
    13381 cgctaccagc aatgcaaaac caaaacccgc cgcccgtgcc gggacgttta gtgacatctc
    13441 tgcgaaggtg tgatgcagaa tcgaaccact ggcagaaata taggcatagg tcaggatata
    13501 gagcacaaag gcaatggaaa tgccgttgac cacgttccag cctttgccca gcaaatcttt
    13561 ggtgatggtg tcaaaactcg aaccgattct gtaattcagg ttagcttcca gaatcatcaa
    13621 gccggaatgc agcatacaga accaggtaaa gatcagcgcc gccattgacc agaaaaacca
    13681 cgccccggac atgaccactg gcagagaaaa catccctgcg ccaataatgg tgccgccgat
    13741 aatcaccacg ccgccaagca gcgacggtga cgtttgggtg gtggttagtg ttgccatgag
    13801 ggcttctctc cagtgaaaaa tagtgcgact gcgttgttat gtattgcact gtaccagtac
    13861 gcgagtacaa aagacagaaa aaaagccccg atggttcaaa tcggggctgt atatattatt
    13921 ttacagactg tgttcgctgt tcagcgatga ttacgcatca ccaccgaaac gacgacgacc
    13981 ggtagagtca tcacgacgcg gagcgcggcc ttcacgacgt tcgccgctaa aacgacgacc
    14041 atcaccacga ccaccttcac ggcgttcacc gctgaagtta cgaccacctt cacgacgctc
    14101 gccactgaaa ccacgaccac cgccacgacg ctctccgcca gtatgcggct gtgcatcgcc
    14161 cagtaattgc atattcatcg gcttgttcag aatgcgagta cgtgtaaagt gttgcagcac
    14221 ttcacccggc atacctttcg gcagttcgat ggtggagtga gaagcaaaca gcttaatgtt
    14281 accgatgtaa cggctgctga tgtcgccttc gttagcaatc gcaccaacga tatgacgaac
    14341 ttcaacacca tcatcgcggc ccacttcaat acggtacaac tgcatgtcac caacatcacg
    14401 acgttcacga cgcggacgat cttcacggtc gccacgcgga ccacggtcgt tacgatcgcg
    14461 cggaccacgg tcatcacggt cacggaattc acgtttcgga cgcatcggcg catctggcgg
    14521 aacaatcaga gtacgttcac cctgtgccat tttcagcagt gcggcggcca gagtttcgag
    14581 atccagctct tcaccttctg cagtcggctg aattttgctc agcagtgcgc ggtattgatc
    14641 cagatcgctg ctttccagct gctgctgtac tttagcggcg aatttttcca gacggcgttt
    14701 gcccagcagt tctgcgttcg gcagttctac ttccggaata gtcagcttca tagtacgttc
    14761 aatgttgcgc agcagacgac gctcgcggtt ctcaacgaac agcagcgcgc ggccagcacg
    14821 acccgcacga ccggtacgac cgatacggtg aacgtaagac tcagaatcca tcgggatatc
    14881 gtagttaacc accaggctga tacgctcaac gtccaggcca cgggctgcaa cgtcggtcgc
    14941 aatcaggatg tccagacgac catctttcag gcgttccagt gtctgctcac gcagcgcctg
    15001 gttcatgtca ccgttcagcg cggcgctgtt gtagccgtta cgctcaagag cttcagccac
    15061 ttccagagtc gcatttttgg tacgaacgaa gataatcgcc gcatcaaaat cttccgcttc
    15121 caggaaacgt accagtgctt cgtttttacg cataccccag acagtccaat agctctggct
    15181 gatgtcagga cgggtagtca cgctggactg aatgcgcact tcctgcggct ctttcataaa
    15241 gcggcgggta atgcgacgaa tcgcttccgg catggttgca gagaacagag cggtctgatg
    15301 accttccggg atctgcgcca taatagtttc aacgtcttcg atgaagccca tgcgcagcat
    15361 ttcgtcagct tcatccagaa ccagaccgct cagtttagag aggtccagag tgccacgttt
    15421 caggtggtcc agcagacggc ccggagtacc gacaacgatc tgcggcccct gacgcagggc
    15481 gcgtaattgc acgtcataac gctggccgcc gtacagggca accacattta cgccgcgcat
    15541 gtgtttagag aaatccgtca ttgcttcagc aacctgtacc gccagttcgc gggtcggtgc
    15601 tagcaccaga atctgtggtg ctttcagctc aggatcaaga ttttgcagca gaggtaaaga
    15661 gaatgctgca gtttttccgc tccccgtctg ggccataccc agtacgtcgc ggccattcag
    15721 cagatgtgga atacactccg cctgaattgg agatggtttt tcgtaaccca gatcgttaag
    15781 ggcttcaagg ataggagcct tcaggcccag atctgcaaaa gtggtttcga attcagccat
    15841 gtagtacgtg tgcctcaaaa ttaatggcgg ccagtctaca taactcatca tgaaattgat
    15901 cagcaatttt cattgaaaag tgtgaaccgg ctcaaagtag gtgtattaac gaacaacaac
    15961 gccctcaccc gttaaggtga tggcaatcaa aaaagattac gggctgatgt gtacgtcagc
    16021 tattgctggt ccgattctgc caggtcatct tggtcctggc ccaggagcga taattccaac
    16081 aatgcgtatc ggtgctcaac aaagttatga acgttgttgg caaccgccag tttgaacagt
    16141 gccgtggcgc tgtccaaatc ccccagactt aggtagtact tacctaaata gaagttggtt
    16201 tcactgagat gctcagcgag cgaggtgtta tccgttgcgt ccgccttgag cctttccatt
    16261 aacgtttgtt cgctaatgtt gcccaggtag aactcgacaa tgttccatcc ccactgttcc
    16321 ttatccgatt tttcgaagtg ctgtttcaac acttctttag cctgcttctc atcgagcttc
    16381 tgctcggcga gataaagcca cagactacgg aaaggatcat tgggatcgtc ttgataaaac
    16441 gccagcagat catcttgcgc taacttgtca cgaccaccgt aatataatgc gatcccgcga
    16501 ttcaagtgcg cgtagttgta agttggatca agctcaagta cagaatcaaa cgcttcatag
    16561 gcagcatcaa aattgcctgc ctgcgttaaa tatatgccta agtaattgaa tacttcaggc
    16621 atatccggtc ggattgccag cgcttgcgaa aaatcgttac gcgctaatgc cctcagaccg
    16681 agactatcat acaacactcc gcgctcatat aaaagctgtg cgcgttcgtc atcggttaaa
    16741 gcccgactgg caaggatttg ttccatacgt gccagaatca cttcctgctg taaagtcggt
    16801 tgcaatggta ccgcgaggac ttcactttta cgccaggaag tattactgca tcctgcaagc
    16861 gtaagtgctg tcgcaacgaa acaccagcgc aaaaaaggct tcatttccca ctcccgaaga
    16921 ccacggttga atgaacgtcc tgttcccggt tgctaacaag gcgtcctgcc cggttaaaag
    16981 ccccccgccg cagcggaggg caaatggcaa ccttactcgc cctgttcagc agccggagct
    17041 tccggtgctg cagcaggttg agactgctca gtcgcttctt taatgctcag acggatacgg
    17101 ccctggcgat caacttccag aactttcacc ggtacttcct gacccatctg caggtaatcg
    17161 gtcactttct caacgcgttt gtcagcgatt tgagagatgt ggaccagacc ttctttaccg
    17221 ccgccgatgg caacaaatgc gccgaagtca acgatacggg tcactttacc agtgtagacg
    17281 cggcccactt caatttctgc agtgatctct tcgatacgac gaatagcgtg tttcgctttc
    17341 tcgccatcgg tcgctgcgat cttcacagta ccgtcatctt cgatttcgat ggtggtgcca
    17401 gtttcttcgg tcagggcacg gatcacagag ccgcctttac cgataacgtc tttgatcttg
    17461 tccgggttga tcttgatggt atggatacgc ggagcgaact cagagatatc gccacgtggc
    17521 gcgttgatcg cctgttccat tacacccagg atgtgcagac gcgcaccttt agcctggttc
    17581 agtgcaacct gcatgatctc tttggtgata ccttcaattt tgatatccat ctgcagtgca
    17641 gagataccgt cgcgggaacc cgcaactttg aagtccatat cgcccaggtg atcttcgtcg
    17701 cccaaaatgt cagacagtac aacgtagttg tcgccttctt tcaccagacc cattgcgata
    17761 cccgcaacgg cagctttgat tggcacacct gcgtccatca gcgccagaga cgcaccgcac
    17821 acggaagcca tagaagaaga accgttggat tcagtgattt cagacacaac acgtacggtg
    17881 tacgggaatt tgtccatatc cggcattact gccagcacgc cgcgcttcgc cagacgaccg
    17941 tgaccaattt cacgacgctt cggagaaccg accatgccgg tttcgcctac ggagtacgga
    18001 gggaagttgt agtggaacag gaaggtatcg gtacgttcgc ccatcagttc atcaagaacc
    18061 tgcgcgtcac gagcagtacc cagcgttgcg gtaaccagcg cctgcgtttc accacgggtg
    18121 aacagcgcag aaccgtgagt acgcggcagc acgccagtac gcacatccag accacggatc
    18181 atatcttttt cacgaccatc gatacgcggt tcgcctgcca gtacgcggct acgaacaacg
    18241 tttttctcga tcgcgtgcag aatttcaccc agttcgtttt cgtccagggt ttcgtcttca
    18301 gcaagcagcg tcgcgatggt ttcagatttg atgacatcaa cctgcgcata acgctcttgt
    18361 ttgtcggtga tgcggtaagc atcgctcaga cgagcttcag ccagtgcagc aacgcgcgcg
    18421 ttcagcgctt cgtttaccgg ctccggctgc cagtcccaac gcggtttacc ggcttctttc
    18481 accagttcat tgatgttctg aataacaacc tgctgttgtt catgaccgaa cactactgcg
    18541 cccagcatct ggtcttcgct cagcagttca gcttcagatt caaccatcag tacagcggct
    18601 tcagtacccg caacaaccag atccagtttg ctctctttca gctcgtcctg agtcgggttc
    18661 agtacgtact ggtcattgat gtaacctacg cgggcagcac caatcgggcc attgaacgga
    18721 ataccagaca gagacagtgc tgcggaagca ccaatcatcg cgacgatatc cgggttaacc
    18781 tgcgggttaa cagaaaccac ggtggcgata acctgaactt cgttgacgaa gccttccggg
    18841 aacagcgggc gaatcgggcg gtcaatcaga cgcgcgatca gggtttcgcc ttcgcttgga
    18901 cgaccttcac gacggaagaa gctacccggg atacgaccag cagcgtaggt acgctcctga
    18961 tagttaacgg tcagtgggaa gaagtcctga cctggtttgg cttttttctg gccaacaacg
    19021 gtaacaaata ccgcggtgtc atccatgcta accataacag cggcagtagc ctgacgagcc
    19081 atcatgccgg tttccagagt cacggtgtgt tggccgtact ggaatttacg aacgatcgga
    19141 ttaagcaatg taatatcctt tctctttctt agacagtacc ttacggcact ggtgttaata
    19201 cccgatcttc tgcgcatcct cgcgactaat gacaacccta acccagctct atgtgggtaa
    19261 agcctctcat tagccgcgcg aacctctgca acggaagatc attcatagca acaatacatt
    19321 agtttccagt gaattgctgc cgtcagcttg aaaaaagggg ccactcaggc ccccttttct
    19381 gaaactcgca agaattagcg acgcaggccc aggcgctcga tgagccgggt gtaacgtgct
    19441 acgtctttac gtttcaggta gtcgagcagt ttacgacgct gagaaaccat gcgcagcaga
    19501 ccacgacggc tgtggtgatc ttttttgtgc tctgcaaagt ggccctgcag gtggttgatc
    19561 tgtgcagtca gcagtgctac ctgaacttcg gtagaaccgg tgtcgtttgc gtcacgacca
    19621 aactcagaaa cgattttagc tgttgcttca gtacttagag acattttaaa actccaaagt
    19681 atatagaatg aaaggacgcc gatctctaat tcagcgatcc cagtgtacgt tacgcaaagt
    19741 gttaaacaat ttacgcgacg ttaagcggca gtattctact cgtagcgacc tgttatcgca
    19801 agacggttaa cattacgccg ggtattcaac caccagacga cgaggcgcaa cgcggccttc
    19861 atcgtcaatt tcgcccatac cgataaactt gccgttctca ccttccgtaa cgcgaaccag
    19921 tccttccagc ggcgcgccag atgtacgaac cgggttacca tttttgaagt aaacagaaga
    19981 cgttaacgga agattcacca ccgggtagtc cgaagctgga ctgtccattg gcatcagtaa
    20041 tggatcaagc aactccgcag ccggaatatc ctgctgttcg gcttgttcga ccagttcacg
    20101 caggtgctcc agggtcacca tccgttcaac cggatattta cttaccgcca gacgacgcag
    20161 gtaaatcaca tgcgcgccac agccgagttt ttcacccagg tcatcaatga tggtgcggat
    20221 ataagtgcct tttgagcagt gaatttccag ctccagctca ttgccttcat ggcgaataaa
    20281 cagcaattca taaacggtaa ttggacgcgc ttcacgcgga acttcaatgc cctgacgcgc
    20341 atattcgtac agttttttgc cctggtattt cagtgctgaa tacatcgatg ggatctgttc
    20401 gatatcgcca cggaaagtat ccagtgccgc cgccagttgt tctgcactaa aggttaccgg
    20461 acgctcttca acgatttgtc cgtcggcgtc agaagtatcg gtacgctgtc caagacgcgc
    20521 aatgacccga tagcgtttgt cggagtccag cagatactgg gaaaacttcg tcgcttcccc
    20581 gaggcaaatc ggcaacatgc cggtcgccag cgggtccagc gcaccggtat gcccggcacg
    20641 attggcgtta tatatacgtt tcactttttg cagcgcatcg ttgctggaca taccctgcgg
    20701 tttatccagc aacaaaacgc cgttaatgtc gcgaccgcga cgacgaggac gactcattag
    20761 tcctccttgc tgtcgtccgg gttaacacga cgttcttcgt catgtttgac cacgctggtc
    20821 accaggtttg acatacgcat cccttcaacc agagagttgt cgtagaagaa ggtcagttcc
    20881 ggcacgatac gcaggcgcat cgctttaccc agcaggctgc ggatgaaacc agaagcttcc
    20941 tgcaacgctt tgatgcccgc tttaaccgcg tcttcatctt tgtcgttgag gaaagtcaca
    21001 tacactttgg catacgccag gtcgcgagac atttcgacac cggaaacggt ggtcatcatg
    21061 cccagacgag gatctttaat ttcacgctgc aggatgagag cgatctcttt ttgcatttcc
    21121 tgcgctacgc gctgcgggcg accaaattct ttcgccataa taaattctcc tgacaaaaaa
    21181 ggggctgtta gccccttttt aaaaattaat ttcaggtgga agggctgttc acgttggcct
    21241 gataagacgc gccagcgtca catcaggcaa tccatgccgg atgcggcgta aacgccttat
    21301 cccgcatgga accctaaaaa ccttaagcga tggtacgttg gatctcgatg atttcgaata
    21361 cttcgatcac atcgccagtg cggacgtcgt tgtagttctt aacgccgata ccacattcca
    21421 taccgttacg gacttcgtta acgtcatctt tgaagcggcg cagggactcc agctcgcctt
    21481 catagataac cacgttgtca cgcagaacgc ggatcgggtt gtgacgttta accacacctt
    21541 cggtaaccat acagcctgcg atggcaccaa atttcggcga tttgaacacg tcacgaactt
    21601 ccgccagacc gataatctgc tgtttcagtt ccggagacag cataccgctc atcgccgctt
    21661 tcacttcgtc gatcaggtta tagatgacgg agtagtaacg cagatccagg ctttccgctt
    21721 caatcacttt acgtgcagag gcatcagcac gtacgttgaa gccaaccagg atggcgttgg
    21781 acgccgcagc caaggtggcg tcggtttcgg tgataccacc tacgccagaa ccgatgatct
    21841 tcactttaac ttcgtcagta gacagtttca gcaaggagtc ggagatcgct tcgacagaac
    21901 cctgtacgtc tgccttcagg acgatattca cttcgtgaac ttcgccttcg gtcatgttgg
    21961 cgaacatgtt ctcgagttta gatttctgct gacgcgccag tttaacttcg cggaatttac
    22021 cctgacgata gagtgcaact tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat
    22081 cacccgcagc cggaacaccg gacaagccga ggatttccac cggaatggac ggacccgctt
    22141 ccagcacttc ctgacccagt tcgttacgca tcgcacgaac acgaccgtat tcgaagccac
    22201 acagaacgat atcgcccttg tgcagagtac cttcacgtac cagaacggta gcaaccggac
    22261 cacgaccttt atcgaggaag gattcgataa ccgcaccgct cgccatacct ttacgtaccg
    22321 ctttcagctc cagaacttcc gcctgcagca ggatagcgtc cagcagttca tcgataccgg
    22381 tacccgcttt cgcagatacg tgtacgaact ggctttcacc gccccactct tccggcagga
    22441 tgccgtactg ggagagttcg ttcttaacgc gatccggatc ggcttctggt ttatcgatct
    22501 tgttcactgc aaccaccacc ggtacctgcg ccgctttcgc gtgctggatt gcttcgatgg
    22561 tctgcggcat tacgccgtcg tcggcagcaa caaccaggac tacgatgtca gttgcctgcg
    22621 caccacgtgc acgcattgaa gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca
    22681 tgccgttttc agtttcaacg tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc
    22741 cagaggccac tttcgttgaa cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt
    22801 gacccatgat ggtcacaacc ggagcgcgcg gttcagccgc agctcccgtg tcacggtcgc
    22861 tcattaccgc ctcttccagc tcgttttcac gacgcaggat aactttgtgg cccatctctt
    22921 cagcaaccag ctgtgcggtt tcctgatcga taacctggtt gatggttgcc attgcgccca
    22981 gtttcatcat cgctttgatg acctgagagc ctttaaccgc catcttgttc gccagttcgc
    23041 caacggtgat agtttcgccg atcacaacgt cacggttaac ggcctgagca ggcttctgga
    23101 agccttgctg cagcgaagaa cctttacgtt ttccgccctt accgccacgt actgctgcgc
    23161 gtgcttcttc acgatcagct tttgattcag cgtgtttgtt gcctttcttc ggacgcgctg
    23221 ctttcgcgtt acgaccacgg ccacggccgc cttcgacttc acgatcgctt tcgtcttctg
    23281 cctggcgagc atgttgagaa gtagtgacgt gataatcgct ggaatcctca gtcggttccg
    23341 cgttatcagt ccatttgttt tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg
    23401 cttcttcttc gagtttacga cgcgcttctt cttcggcttt acgcttgagc tctgcagctt
    23461 cctgctcacg gcgggctttt tcagcctggg cgtttttagt catatcgtct tgttgattgc
    23521 tcactttgtc tttttccgca gcttcacgtt tcgcttgttc agcagcttca cgcttagctt
    23581 gttctgcggc ctcacgttca gctttttgtt gcgcctcgcg tttagccgat tcttctgcct
    23641 cacgacgggc ttgctcttcc gcttcacgct gcgcttgctc ttccgctgca aggcgttcag
    23701 cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt
    23761 tgctttttcc accggtacca ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca
    23821 atttgtccgg gcctgaattt ttctgattca ggtggtcaat caaagtctgt ttctcttgtg
    23881 cagacacaga gtcgtcagca gacttccgga tacctgcatc agcaaattgc tgtaccaggc
    23941 gttccacgga ggtctgtcgc tctgcggcca gcgttttaat cgttacatct gtcatgctgt
    24001 tccttcctgc tacagtttat tacgcttcgt caccgaacca gcaaatatta cgggcagcca
    24061 taatcagtgc tccggctttt tcgtcggtca acccttcgat atcagccaga tcatcaatgc
    24121 cctgttcggc gagatcttcc agcgtacaaa cgccacgggc ggccagtttg aatgccaaat
    24181 cacgatctac cccttcaagg ttcagcagat cgtcagccgg tttgttatca ccgaggcttt
    24241 cttcctgggc ctgtgcaatg gtggccagtg catttttagc acgctcgcgc agtgcttcaa
    24301 cggtcggctc atcaaggcct tcgatttcca acagctcttt catcggcaca taggccagtt
    24361 cttccagcgt cgagaagcct tcctctacca gaacagtcgc gaagtcttcg tcgatgtcga
    24421 gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt
    24481 cgtcaacggt catcacgttg agttcccaac cgctcagctg cgaagccaga cgcacgttct
    24541 gaccgttacg gccaatcgcc tgcgccaggt taccggcttc aacggcgata tccatggtgt
    24601 gtttatcttc atccaccacg atagaagcaa cgtctgccgg tgccattgcg ttaatcacaa
    24661 actgcgccgg gttatcatcc cacaggacga tatcgatacg ctcgccgccc agttcagtag
    24721 aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc acctaccgga tcgatacgtt
    24781 tatcgttggt tttcaccgcg attttcgcac gagaacccgg gtcgcgagcc gctgctttaa
    24841 tttcaatcac ttcttcgccg atttctggca cttcaatacg gaacagttcg atcagcattt
    24901 ccggcttgga acgagtgacg aacagttgcg cgccacgcgc ttccgggcga acggaataaa
    24961 gcacgccacg aacgcggtcg ccagggcgga agttttcacg cggcagcata tcttcgcgca
    25021 ggatcacggc ttcagcgttg ttgcccagat ccagagagat gttgtcgcgg tttacttttt
    25081 tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc accatcgcac
    25141 gttcggcttc acgcactttc tgcacgataa cctgttttgc cgtctgggtg gtgatacggt
    25201 caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt
    25261 cataacgtgc ggcttcaagg gtgatttcct tggtcggctg ggtgacttca tcaacaacta
    25321 accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc gatctgtacg cggacgtcga
    25381 tctcttgttc atattttttc tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa
    25441 tcttctcgcg aggtagcgcc ttttcattgg atacggcttc aactacagcc aaaatttctt
    25501 tgttcatcgc gggcttttca cctcatccag actattaaaa gtggggaacc aagttcgcct
    25561 tctggatatt actcagcgcg aacacttcat ctttaccttc gacggtaact gtgatcattt
    25621 caccgtctac cgctttgata acgccctgcc atttacgacg gttttgtacc gccatacgga
    25681 gaaccagagt cacctcttct ccgacaaaac gggcgtagtg ttcagccgtg aacagtgggc
    25741 gatcgagacc cggtgaggag acttccaggt tataagcaac ggtgatggga tcttcaacat
    25801 ccagcacagc acttacctgg tggctcacat cagcacaatc atcaacattg atgccatctt
    25861 cactatcaat atagatgcgc agtgtggatg tgcgaccgcg aataaattcg atgccaacca
    25921 gttcaaaacc cagggcctca actggcgcag taatcatctc tgttaatttt tgctctaatg
    25981 tggacaagcc cacccccaag acataaaaaa agggcctaaa gcccagttat tctgtagtca
    26041 gataacaaaa aaccccgata aatcggggct ttatataact gaaccctata accgcaactg
    26101 cggtctggag cactttccag aaggattttt tcaaatccca ctacgaaggc cgaagtcttc
    26161 acagtatatt tgaaaaagga ctctaaggga aagtggttgc gggggccgga tttgaaccga
    26221 cgaccttcgg gttatgagcc cgacgagcta ccaggctgct ccaccccgcg cctgaaacgt
    26281 ggcaaattct actcgttttg ggtaaaaaat gcaaatactg ctgggatttg gtgtaccgag
    26341 acgggacgta aaatctgcag gcattatagt gatccacgcc acattttgtc aacgtttatt
    26401 gctaatcacg tgaatgaata tccagttcac tttcatttgt tgaatacttt tgccttctcc
    26461 tgctctccct taagcgcatt attttacaaa aaacacacta aactcttcct gtctccgata
    26521 aaagatgatt aaatgaaaac tcatttattt tgcataaaaa ttcagtgaga gcggaaatcc
    26581 aggctcatca tcagttaatt aagcagggtg ttattttatg acgacgattc tcaagcatct
    26641 cccggtaggt caacgtattg gtatcgcttt ttccggcggt ctggacacca gtgccgcact
    26701 gctgtggatg cgacaaaagg gagcggttcc ttatgcatat actgcaaacc tgggccagcc
    26761 agacgaagag gattatgatg cgatccctcg tcgtgccatg gaatacggcg cggagaacgc
    26821 acgtctgatc gactgccgca aacaactggt ggccgaaggt attgccgcta ttcagtgtgg
    26881 cgcatttcat aacaccaccg gcggcctgac ctatttcaac acgacgccgc tgggccgcgc
    26941 cgtgactggt accatgctgg ttgctgcgat gaaagaagat ggcgtgaata tctggggtga
    27001 cggtagcacc tataaaggaa acgatatcga acgtttctat cgttatggtc tgctgaccaa
    27061 tgctgaactg cagatttaca aaccgtggct tgatactgac tttattgatg aactgggcgg
    27121 tcgtcatgaa atgtctgaat ttatgattgc ctgcggtttc gactacaaaa tgtctgtcga
    27181 aaaagcttac tccacggact ccaacatgct tggtgcaacg catgaagcga aggatctgga
    27241 atacctcaac tccagcgtca aaatcgtcaa cccgattatg ggcgtgaaat tctgggatga
    27301 gagcgtgaag atcccggcag aagaagtcac agtacgcttt gagcaaggtc atccggtggc
    27361 gttgaacggt aaaaccttta gcgacgacgt agaaatgatg ctggaagcta accgcatcgg
    27421 cggtcgtcac ggcctgggca tgagcgacca gattgaaaac cgtatcatcg aagcgaaaag
    27481 ccgtggtatt tacgaagctc cggggatggc actgctgcac attgcgtatg aacgcctgtt
    27541 gaccggtatt cacaacgaag acaccattga gcagtatcac gcgcatggcc gtcagttggg
    27601 ccgtctgctg taccaggggc gttggtttga ttcccaggcg ctgatgctgc gtgactctct
    27661 gcaacgctgg gttgccagcc agatcactgg tgaagttacc ctggagctgc gccgtgggaa
    27721 cgattattca atcctgaata ccgtctcaga gaacctgacc tacaagccag agcgtctgac
    27781 gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt atcggtcaac tgaccatgcg
    27841 caacctggat atcaccgata cccgcgagaa acttttcggt tatgccaaaa ctggcctgct
    27901 ttcctcctct gccgcttcag gcgtgccgca gatggagaat ctggaaaaca aaggccagta
    27961 attcgacatc aaccctgccc ttcaggggtg gggttacctt gcttatcctc tgtaaactcc
    28021 tttctgagca tcacataatc gaaaacatca tggtttgcat ccacaaccag tgagtctcct
    28081 ttatagtgta caatcacttc ctcaagcgtt tgcggctgtt ccggctttgt tacccccatc
    28141 cacgcattaa taaggtaatt attgtaagct gtcgaaaaga tgttgttttc cgttgtgcga
    28201 tccacaccat ccccaagaac ggggctatac cagataaaca tcggaacatg atatgcctgc
    28261 tggctggcct ccctgcctcc atgaaaatag acgttcttct tcgtagggtc acgttccagg
    28321 ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt ttaataattc aaaaacctga
    28381 cctagcaaac tatctgtgta atgaatggag ttgtcatagc aggcatcctg atcgtcctgc
    28441 ggttgaaaca cggcgctgga ttgcggatag gcgctacaag ccggttcatg gcttccattt
    28501 aaatgaagaa caatcagttt cttttgctgc gtattttgct gtaatgcttg cgataaatgc
    28561 ggcaacaaca attcatcaaa tcctctgaca taaactgttt ccatggcacg catggcgata
    28621 ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga
    28681 aatcctgctt gattagccat attaataatg ttgtcggggt aattatgaat gtcatggctc
    28741 aaaacagaat cagcagttaa agaaagggga accgacagcg cagtgtaagg tgcaccgctt
    28801 attgcttgat taaacagttt gatctgtttt ctttgtgctt cgacttgcgg tgtcgtagag
    28861 cgtgtatatc catacaaaga catattgtcg acacgtacag actcccccac aatcaacacg
    28921 taggtatcaa tacctgtatc cctgactgat aattgaaaat acggcactgt gtttgcaatt
    28981 gagagtaatc tttgatgctc tttcgctgct aaagcaaaat aattgagatt gaaaaacggc
    29041 gtataggtag caaatcgcga cgctagtata tatggactga acgcattttt atttttggca
    29101 tctttataag caaattgaca agcggaaaat aaacttcccg aaatgacaat cagcaataat
    29161 attcctgtca ctttttttgt cggcaaggaa acatcatatt ttattattac agccaaaaaa
    29221 agaagggata aaaaggcaaa agcacataga taagggctat acattcccag cattttggca
    29281 acttcatccg gatcactctg cagcacacta atcgcaaatc catcattgaa agttgtacca
    29341 aaggtacacc atgaatagag gctaatactc atgtctgccg cagtcagtaa cacaaatggt
    29401 gcggcaacaa gcaggcgaag cggaagggat gatatacgtt taacaaccag gatggtcaga
    29461 acgaagaaca gcaatcgctg aaccatatgc gctccggaag aggcgactaa gttcgtaata
    29521 ccaaaaacaa taacactaag atacaacaac caatgagatt taaaacttct agcaaatttg
    29581 ttgaatactg tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt
    29641 gcaattttat atcagacatg tttttcaact ctgatccata tcagaacgaa acactgttag
    29701 ttttttcttt ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg
    29761 cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg
    29821 tttatcgaga agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg
    29881 ccaattatgt cagcagtagg gaaaagttta taacgaaggc gtaccggcac catcgtttca
    29941 aggtaccagc tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca
    30001 gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg
    30061 aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta
    30121 ccaattccac cacctcggca cggaaactac tttttagttc gggatatcgc tggtcggctt
    30181 agccggagca gctggctgag tttgttcggt tttcgccggc gcactcaggt tttcccattc
    30241 gctaccttta ttggtcttgt tgctattgat gttacccagc accagactga tgatgaagaa
    30301 taacgttgcc agcaacgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa
    30361 cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg
    30421 tagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacca aaagagcttc
    30481 gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc
    30541 gggagtttat aatctttccc actgaagcgg gtgtgaatac taaccaaaac gaatgacctt
    30601 cgcaagggca attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt
    30661 tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca gttatctaac gctttaaacg
    30721 gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct
    30781 tccaccatca cgcgaattaa cggttcggtg ccggatttac gcaacagcac acgtccacgg
    30841 ctacccagcg ccgtttcaac ttctgcggtc accgctttga ctgattcatg ctccagcgga
    30901 tcgccgctac ctgcggtgta acgcacgttt accaggattt gcgggaacat tttcatgccg
    30961 ctgcaaaggt cgtgcaggct catatggtta cgcgccatcg ccgccagcac ctgcaaacca
    31021 gcaacgatgc cgtcaccggt agtggtttta tccagcagga tcacatgacc ggaattctct
    31081 gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc
    31141 gcgcgcgcaa atggaattcc taactgtttc agcgccagtt caagccccat gttgctcatc
    31201 aatgtaccca cagcgccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata
    31261 tacatgatct gatcgccatc gactttattg ccttcatggt caaccataat cacgcgatcg
    31321 ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc
    31381 gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgtttgg ctcacaaccg
    31441 atagcgataa cattcgctcc cagttcgcgc agcacgttcg gcgcgatgtg ataagtcgca
    31501 ccgtttgcgc aatccaccac aatcttcagt tcactgaggc taagttcgtt cgggaacgtg
    31561 gctttgcaaa actcgatata gcgacccgca gcatcaacga tacggctggc tttacccagt
    31621 tctgccgaat caacacagct gatctctttt tccatttccg cttcgatggc ttcttcaact
    31681 gcatccggca gcttagtacc gtcaatagaa aagaatttaa tgccattatc gtagaacggg
    31741 ttatgcgatg cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc
    31801 acggccggtg ttggcatcgg gccagtgaag agtgcggaaa ggcccgcagc cgccagcccc
    31861 gcttccagtg ctgactccag catatagcca gaaatacgcg tgtctttacc aataataatc
    31921 ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa
    31981 tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta
    32041 cgattactca tagcgtttgt tttcctttgc agacagagta gcttccacca ctcgcatcgc
    32101 ttctacggtt tctttgacgt catgaacacg aataatgtgc gcgccttgca ttgcggcaat
    32161 gaccgcacag gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc
    32221 aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt gaaattcagc
    32281 caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc
    32341 gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa
    32401 gtagcgattc acttctgcaa agacatcgtc atacttcggg gcttcctgca tggtttttgg
    32461 atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc
    32521 gccaggttcg gaaagggagc ggatatcgtt aataatgtga gcgccaactt tcgctgactc
    32581 acggatgact tctggtttgg atgtatcgac cgagatccag acttcgaagc gttgagcaat
    32641 tgcctcaacc acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc
    32701 tggccgcgtg gactcgccac caacgtcaat gatcgtcgcg ccagcgttga tcatcagatt
    32761 cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg
    32821 cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag taccctgggc
    32881 aaagagtttc atgatgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc
    32941 ccggggtttt cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc
    33001 tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt
    33061 ggactaccat tgtcgccagc attgttagaa gcgcctggtt cttcccagcc cgctggcgga
    33121 cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc
    33181 atgagagcat ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca
    33241 cgattatagt tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca
    33301 tcggacatat gtttcgcttt cgctacgcta cggccgagga acacttcacc ttcttcttcc
    33361 gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat gttacgtgcc
    33421 aggttggtcg caactttaat atcgttggac gcaccggtag aaacatgttc cggcccgtag
    33481 atgatctctt ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga
    33541 cggctggcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg
    33601 cgtgggataa tcgtcacttt gtgcaccgga tcgtgctccg gcaccaggcg accgataatc
    33661 gcatgacccg cttcgtggta agccgtcgat tctttctgcg cttccgtcat caccatggag
    33721 cgacgttccg cacccatcat gattttgtct ttcgctttct cgaactcaac catcgacaca
    33781 acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca
    33841 gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc gggtgccaat
    33901 ggtacgcgac gcatgtgaac cttcaggatc tgctcacgac cgcgaacatc tggcaagcca
    33961 accacaacct gacggtcgaa acggccagga cgcagcagcg ctgggtcgag aacgtccgga
    34021 cggttagtcg cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc
    34081 agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccagacc tgcgccacgc
    34141 tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc
    34201 tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca
    34261 gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcagc
    34321 gttttaccgg tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt
    34381 ttctggaagc ggctcggctc gcgcagatac tcaaccagtt cagcaacttc ttcttttgct
    34441 tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc
    34501 gctttactct taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg
    34561 aagaagatcc agacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc
    34621 agcaggctgg gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta
    34681 tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg
    34741 gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct
    34801 tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg
    34861 aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca
    34921 ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc
    34981 cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca
    35041 atatacactt cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta
    35101 aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact
    35161 acaaaactgc cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga
    35221 tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac
    35281 atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca
    35341 tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg
    35401 caagcgatga tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc
    35461 ggagcagcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt
    35521 atttcatcaa gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca
    35581 tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct
    35641 gtcatttaac tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc
    35701 ctatttggtg atatatggga gatggcggta gaatgacccg ttttcaatcc caacgtaagc
    35761 aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc
    35821 gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat
    35881 tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga
    35941 aactaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt
    36001 catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg
    36061 ctaagattat cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca
    36121 ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gctatgcggc ctttttcctt
    36181 tctttacaat acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat
    36241 tacttcaaat tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt
    36301 gccgatcagg ccacgagcaa taggtgagtt tacagaaatc aggttttgtt taaagtcagc
    36361 ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca gaatcgagat tcagcaccgt
    36421 tacggtagca ccaaaaataa cgcgcccatt gttgggcatt ttggtgacat caatcacctg
    36481 cgcgttcgac agcttggctt cgatgtcttt gatacggcct tcgcagaagc cctgctgttc
    36541 acgagctgcg tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat
    36601 agcagcaatg atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt
    36661 ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct
    36721 gatggggcaa gtgttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg
    36781 gaccagaagc aaaaaaatac cgacccgggt acaagtccca ggtcagctac aattcacatt
    36841 ttgatagtca ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc
    36901 gccgtagtat gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt
    36961 tatcatcgga ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg ttgatgagta
    37021 cattactcaa ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc
    37081 ggcccccgct attgattacc acagtcagca gatggcgctg cccgccagta cccagaaagt
    37141 gattactgcg ctggcggcgt tgattcaact cggccccgat tttcgtttca ccacgacgct
    37201 tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg
    37261 tgccgatccg acgttaaaac gtcaagatat tcgcaatatg gtcgcgactt tgaaaaaatc
    37321 tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga
    37381 taaagccccc ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgt
    37441 cgccatagtt gaccgcaact gtttctccgt ctcgctctac agtgccccaa aacctggtga
    37501 tatggctttt atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac
    37561 cctcccccgt ggttctgccg aagcgcaata ctgcgaactg gatgtggtgc caggcgacct
    37621 gaaccgcttt acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt
    37681 tgccgtgcag gatggagcca gctatgccgg tgcaattctg aaagatgagt taaaacaggc
    37741 gggtatcacc tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt
    37801 agttgccagt aaacagtcgg ccccgctgca cgatctgctt aagattatgc tgaaaaagtc
    37861 ggacaacatg atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtgcc
    37921 tggaacatgg cgggccgggt cggacgctgt gcgtcagatc ctgcgccagc aagccggtgt
    37981 cgatattgga aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc
    38041 ccccgccacc atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt
    38101 tatctccatg ctgccgctag cgggctatga cggctctttg cagtaccgtg caggtctgca
    38161 tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa
    38221 cctggcggga ttcattacca cagcgagcgg gcaacgaatg gcgtttgtac aatatctttc
    38281 tggctatgca gtagaaccgg cggatcagcg taatcgccgt attccgttag tgcgttttga
    38341 aagccgtttg tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg
    38401 gccggggttt tgctgattaa cgcttgtaaa tgaactcaac gccttcttcg tcgtcttcgt
    38461 cccagtcgtc atcccagtcc tcatcatcct cttcagcaat ctcttcaagc tgctggcgat
    38521 gataatcatc ccacatgaat tcgactttct ctggctgttt cgcttcttca gcctgcacga
    38581 ccgggttttc aatgataaag gtcatcacat cccagcagag atctttcacg cccagtccac
    38641 tcgccgcaga gatcagataa tatttatctt cccagcccag cgcctctgcg atcgctttcg
    38701 ctttctcttc ggcttctacc ttatccagca gatcgatctt gttgaagact aaccaacgcg
    38761 gtttcgccgc cagatcctgg ctgtattttt ccagctcgct gataataata cgcgcgtttt
    38821 caaccggatc ggtgccgtca atcggatcga tatcgatgag gtgcaacagg acgcggcaac
    38881 gttccaggtg cttcaggaag cgaatgccca gacctgcgcc ttccgcagcg ccttcaatca
    38941 gtcctggaat atcggcaaca acgaagctct tttcgttgtc cattcgtacc acacccagac
    39001 ttggcaccag agtggtaaac ggataatccg ccactttcgg tttagccgcc gataccgcac
    39061 gaataaaggt cgatttaccc gcgtttggca tccccaacat accgacgtca gccagcagca
    39121 tcagctccag cagcagctca cgcttatcgc ccggcgtacc gttagttttc tgccgtggtg
    39181 tacggttaac tgacgatttg aaacgggtat tgcccagacc gtgccagccg cccttagcaa
    39241 ccagcagacg ctgaccgtgt ttggtcatat cgcccatggt ttcaccagta ccctggtcga
    39301 ttacacgcgt acctaccggc actttaattg tgacgtcttt accgcgctta ccggtacagt
    39361 cgcggcttgc gccattctga ccgcgctctg cacggaaaga tttttcaaaa cgataatcga
    39421 taagcgtgtt caggttctcg tcggcttcca tccatacgtc accaccatca ccgccgtcgc
    39481 cgccatccgg gccgcctttc ggaatatact tttcgcggcg gaagctcacg caaccattac
    39541 cgccatcacc tgcaacgacc agaatcgatg cttcatcaac aaacttcatt ttattctccg
    39601 taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt atgcttacgt aatccgcccc
    39661 aaatacgatg accaatggcg gaatacatcg cgcccgcaac cacgacaaac gcaccgagat
    39721 aacctaaaag gtttaacatc ggtctggcga agaaatcggg ccaggccagt gataaaagat
    39781 ctgaaaaaaa cagcgtaaac agtggggtga gcgtgatgat cgcgctcacc tgcgctgcct
    39841 gccagcgagc catcgcttcc gccagggcgc catatcctac caaggtattc agtccgcaaa
    39901 aaattaaaca tgcgagctgc cagtggctaa gctgcgctat cactccaggc tttgccagag
    39961 ggaagagcgc aattgtacat aaagtgtaca gtaaaaacag gatctgcggt gaggccagcc
    40021 gacgcaataa aaccttttgc gccacgccat aactcaccca aaccgtcgca gcaccgaccc
    40081 caaagataac tccccaggtg taatcggtta gctttgtaaa tatctcgacc agactggtgt
    40141 taaaaaacat caccaggccg ctcaggagca tcaatgcccc tacaacctga gtgctgcgca
    40201 ttttctcttt caggataaat acgctggcaa ccatcatgcc aactggcgag agttgcccaa
    40261 tcacctgcga agcggtcgga ctcaggtatt gcaaggatga gctgaacagg atgaagttcc
    40321 caaacagccc ggcggtcgcc actgccaaca aaatcaacca gcgtggctta cgaaacacgc
    40381 gtaatggcgg caacctcttc ttcaccgcaa gaatggcccc caggccaata ctcgccatca
    40441 agaaacggta aaacacgatt gtcggaggtt ccatcacctc cagcacctgc ttcattgcaa
    40501 ttggcaacgc cccccagcaa attgctgtgg tgagcgccaa aagaatgcca atgcctgcct
    40561 gctgcttcat accgtattcc ctgcaaagcg catttgcgga ttaacgtcgc accatgcgca
    40621 tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt tgcggggctt tcatccgtta
    40681 ccgggacgcg aaaaacttat tcagcttcga tgctgataaa tttacggttt ttcgggcctt
    40741 taacttcgaa tttcacttta ccgtctgctt tagcaaacag agtgtggtca cgaccgcaac
    40801 ctacgttagc gccagcgtgg aatttggtac cacgttgacg aacgatgatg ctacccgcca
    40861 gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt agcttctgaa tcgcgaccgt
    40921 tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa tctctcctca ggtcttaggc
    40981 gctgatgcca gtaattttca catcagtgaa ccactgacga tggccctgct gcttacgata
    41041 gtgtttacga cgacgaaact taacgatttt aactttctcg ccacgaccgt gagcaacaac
    41101 ttcagctttg attacgccgc catcaacgaa aggaacgccg attttgactt cttcaccgtt
    41161 tgcgatcatc agcacttcag cgaactcaac agtttcgcca gttgcgatgt ccagcttttc
    41221 caggcgaacg gtctgacctt cgcttactcg gtgttgttta ccaccacttt ggaaaaccgc
    41281 gtacatataa aactccgctt ccgcgcacac cttttcaatg attcagagtg cgctataaat
    41341 attcacaata gggcgcgaat attacgcaaa acgcacgcct ttgacaagtg ctacagtcaa
    41401 tacacgaaga aaaaaaacac aacttgtacg gtaacgttta tctgtgccat tttttcagta
    41461 caatcaccct atattcctaa ccataaaccc taagttgcct ttgttcacag taaggtaatc
    41521 ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa tcaatgagtt aaccgcgcaa
    41581 gatatggcgg gtgttaatgc ggcaatcctt gagcagctta attccgacgt ccaactgatc
    41641 aatcagttag gctattacat cgtcagcggc ggcggtaaac gtattcgtcc gatgattgct
    41701 gtactggctg cacgagctgt tggctatgag ggaaatgcgc atgtcaccat tgctgccctg
    41761 atcgagttta tccacacggc gactctgctg cacgacgacg ttgtggatga atcagatatg
    41821 cgcaggggta aagctaccgc caacgccgca tttggcaatg ccgccagcgt gctggtaggc
    41881 gattttattt atacccgcgc gttccagatg atgaccagcc tcggttcact caaagtgctg
    41941 gaagtcatgt cagaagccgt aaacgtcatc gcagaaggtg aagttctgca actgatgaat
    42001 gttaacgatc cggacatcac tgaagaaaac tacatgcgcg ttatctatag caaaaccgcg
    42061 cgtctgtttg aggctgccgc gcagtgttcc ggaattctgg cgggctgtac gccggaggag
    42121 gagaaaggcc tgcaggatta tgggcgctat ctcggcactg ctttccagtt gatcgacgat
    42181 ttactcgatt acaatgccga tggcgaacag ttaggtaaaa acgtcggcga cgatctgaac
    42241 gaaggtaaac cgacgctgcc gctgctgcat gcgatgcatc atggcacacc agaacaggca
    42301 cagatgatcc gtaccgccat cgaacagggt aacggtcgcc atcttctgaa accggttctg
    42361 gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc agcgcgccga ggaagaagca
    42421 gacaaagcca tcgcagcgtt acaggtgctc ccggacaccc cttggcgaga agcactcatc
    42481 ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc ctcccctcat cccgcgcagt
    42541 gcgcgggatg attccagtaa actccataag aatacctatt cagcgtaata atgcactgaa
    42601 aaaccagtgc atacatcttt atatattctg aatattcaca ctctttatag gaacttttta
    42661 gagcaatcgg ccatcaggag tatagtgatg ctcgacagaa gaagtgttct gaatgaaagc
    42721 gactaactta aggagtgagg aaagatggaa agtaatttca ttgactggca tcccgctgac
    42781 atcattgcgg gtttgcgcaa gaagggaaca tcaatggcgg cggagtctcg cagaaatggt
    42841 ttgagttcct caacgctggc gaatgcatta tcgcgcccat ggccgaaagg agagatgatt
    42901 attgcgaaag ccctgggaac tgacccctgg gttatctggc catcacgcta ccatgatccg
    42961 cagacccatg agtttatcga cagaacgcag ttgatgcgta gctacactaa accgaaaaaa
    43021 tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc tctcagacga ttattcgcct
    43081 ttcacacgct caatatttgc acctaaagcg cgcagtttgt cttcaatgcg ttcgtatcca
    43141 cgatcgatgt gataaatacg atcaaccacc gtcgtccctt ccgcaataca gccagccagc
    43201 accaggcttg ctgatgcacg cagatcggtt gccataacct gtgcgccaga aagtttttca
    43261 acaccgtgac aaataacggt attgctttcg atttcggcgt gcgcgcccat acggctcagc
    43321 tctggcacat gcataaagcg gttttcaaag accgtttcgg tgatgaatcc agtcccttct
    43381 gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg tcgggaatgc cggatgcggc
    43441 gcggtacgta cgttaacagc cttcggacgt ttgccatgca tatccaggct aatccagtct
    43501 tcgccgacct cgatgtccgc tccagcgtca cgcagtttcg ccagcacggc gtcgagagta
    43561 tctggctgcg tgttacggca gataattttg ccgcgagaaa tcgccgccgc caccaggaaa
    43621 gtaccggttt cgatacgatc cggtagcacg cgatagacac cgccgcctaa acgttccaca
    43681 ccttcgatga cgatacgatc ggtgccctga ccgctaattt tcgcacccag cgtaatcagg
    43741 aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt tttcaataat cgtggtgcct
    43801 tccgccaggg ttgcagcaca catgatggtc accgttgcgc caacgctgac tttatccatc
    43861 acgatatgtg cgcctttcag acgaccatcg acggaagctt taacgtaacc ttcttccagt
    43921 ttgatggtcg cgcctaattg ttcgaggcca gaaatgtgta gatcaaccgg acgcgcaccg
    43981 atcgtacaac cgccaggtag tgaaacttgc ccctgaccaa agcgcgctac cagcggcccc
    44041 agtgcccaga tagaagcacg catggtttta accagatcgt aaggtgcgca gaatacatta
    44101 acgtcgcggg catcaatatg cacagaacca ttacgttcta ctttcgcacc cagctggctt
    44161 agcagcttca ttgatgtatc gacgtctttc agtttcggga cgttctggat ctctaccggt
    44221 tcttccgcca gtagagcggc aaaaaggata ggcagagcag catttttagc gccggaaatt
    44281 gtgacttcgc cctggagctt cgttggcccc tgaacacgaa atttatccat ttagtttgtt
    44341 ctcagttaac aattcatatc cgctaccggc gaatcgccca tagctcaaaa gccgttcagt
    44401 ttgcgatcgc gcgcccactc cgcaggggta tacgctttga tcgacacagc atgaatgcgg
    44461 ttatccgcaa tatattccat cagcggacca tagaccgtct gctgtttttt aacccgactc
    44521 atgccgtcaa acaactcacc cacggcaata acctgaaagt ggctgccatc gccggaaacg
    44581 tggacttcct ggagggagag agcgttcatc aacacgctct gaatttcatt attttccatg
    44641 ggatcttcaa tcatcagtta ataaaccagc gaaacatctt agagcaaagt tgcgctggca
    44701 taaataagca aaaagcctcg ctgataaatc agacaaggct cgacttgcag gcaggtttgc
    44761 cggacaggcg gttaacgcca tatccggcct gaaaaaattt aacgaggcag aacatcagca
    44821 ggcaaattat acaatttcgc cagggtatac actttgtcgt ttaccccctg aagcgtcacg
    44881 ttgttgccct gctttttcgc cagatcgata agatggagca gcagtgccag tccacccgta
    44941 tccacgcggg acacacggct aagatcgatg caggtaatcc ccttcaccgc ttcctcacgc
    45001 atttcccaaa gcggtagcag aacgtcctga tccagctctc cggataacgc cagtgtgtca
    45061 cccgtctgca tccagctcag tgactcgctc attatttttt ctcttccaga gtgattttct
    45121 gttgagaaat cgatttcagt tgcgcagtca ggccgtcgat acctttggta cgtagcagcg
    45181 ttccccactc gttttgtttg gtggtgatca tactgacgcc ttcagcaatc atgtcgtaag
    45241 cctgccagtt gcccgtctgg gagtttttac gccactggaa gtccagacgc accggcgggc
    45301 ggccattcgg gtcaataatg gtaacgcgaa taggcacaat ggttttatca cccagcggct
    45361 gttctggcgc aatctgatag gtttgaccgt gatacatcgc cagcgcctga ccgtaagcct
    45421 gcttcaggta ctcacggaaa gcggcaaagt aagcatcacg ttgagctggg gtcgcactct
    45481 tgtaatactg gcccagcacc agcgcaccgg cgtatttcac ctgtacgtat ggcagcagtt
    45541 cctgatcaac aatggtacgc agataatccg ggttggcccg aatttgcggt tgctcattct
    45601 tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag cttatacgga ttggtctggt
    45661 ctgccgcggt tgccgcactc agcggtgcaa tcaccagcaa agcgaccatc attaaacgtt
    45721 taaacatgcg tcggttctcc tgaaattatt tcgttgtacc cacaggttca gtggtttcat
    45781 tattacctgg cgcagcagct ggcgcatcgc cactattctt attgtcatcg cctttactac
    45841 cgtaaaggaa ctgaccaatg agatcttcca gcaccatcgc agacttggtg tcctgaattg
    45901 tatcgccatc cttcaggata gcagtcccca gttccgggtc ttcaaaaccg acgtttaatg
    45961 ccagatattg ttcccccagc aggccggaag tacgaatgct cagcgaactg gtatcaggaa
    46021 tatggttata acgttgttca atttccagcg ttacgcgcgg cagataggtt ttcgggtcca
    46081 gcgtaatatc cgccacccgg cccacaacaa cgccaccaat actgaccgga gagcgcgctt
    46141 tcaggccgcc aatgttatcg aacgtcgcat aaagcgtgta ggtcggttca gtacgtattg
    46201 acgtcacgtt cgccgccttc aggcaaacaa acagcgccgc cagcagtgct gctaataaaa
    46261 agatacccac ccaaatttca ttttttttcg tttgcatgaa ctcaattccc aaacatcaat
    46321 gcggtcagca caaaatccag ccccagaaca gccagagacg agtggacaac ggtgcgagtg
    46381 gttgcccggc taatcccggc agacgtcggg atggcgtcgt agccgttaaa caacgaaatc
    46441 cacgtcaccg tgatggcgaa caccacgctc ttaatcagac agttgaccag atccatacgc
    46501 cagtcgacgg cattttgcat tgccgaccag aagaacccgc tatcaatgcc tttccaactg
    46561 acgccgacca gcgatccgcc ccagatcccc acggcgacga agataaccgt caacagtggt
    46621 aatgaaataa ccccagccca gaaacgggga gaaataaccc gacgcagcgg atccaccgcc
    46681 atcatctcca tactggagag ttgctctgta gcgcgcatca ggccgatttc tgcggttagc
    46741 gccgaaccag cacgcccggc aaacaacaac gcggcaacca ccggccccag ttcacgcagt
    46801 agcgataacg ccaccagcat acccagactg gtttccgcac tataagtggt cagaaccaga
    46861 taaccttgca gccccaacac cattccgatg aacacgccag aaactacaat aatcagcatc
    46921 gacaggacgc cgacattata gagctggcgc accagcagcg gcgcatgttt gcgaaattcc
    46981 ggtttgccga ccagcgcatt gaataacatt aacccggccc gcccaaacgt tctcagggtt
    47041 ttaatccctt tatgtccgag cgacgccagc gcatttaaca gcatgagtgg cttaactccc
    47101 tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg aacggaaccg gcccgtcagc
    47161 tatcccgtcc agaaactgac gtacgcgcgg atcaggattc gcctgcaacg cctgggcact
    47221 gccatgagcg acaatttttt tgtccgccag gatccaggcg tgatccgcaa tacttaacac
    47281 ttccggcaca tcgtgagaaa ccaccacaca agtcacgccc agcgcgctgt tcagctcaga
    47341 aatcagcttc accagtacgc ccatggtgat gggatcttgc ccaacaaaag gttcatcaaa
    47401 catgatgaga tccggctcca gcgcaatcgc acgcgccagc gctgcacgcc gcgccatccc
    47461 accggaaagt tcagaaggca ttagtttagc cgctccacgc agccccacgg cctccagctt
    47521 catcatcacc gtactatgca acaatggcgc gggaagttgg gtatgttcgc gcagtggata
    47581 ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc ccggactgaa ataacatgct
    47641 catccgtttg cgcactgtat acaggcgcga acgagacatc gccggaatat tctcaccatc
    47701 gaaaaggatc tcaccatgat ctggtgcgat ttgcccgcca atcagacgga gtagcgtcgt
    47761 tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc cctcgcggca cggtcaggga
    47821 aatattatcg aagatgcagc gattgccacg cgtaaaactg acatcgcgca tatcgactaa
    47881 attcgccaca gactgctcca taattcaccc ttcgtcttgc gttgattttc taagcatggc
    47941 gctcaattta accttgaacc caacatattt acagaatatt acccgccgtg gttagcgaaa
    48001 gctggcattt gttttacttt ttagccgcat aaagtcaaaa ttaagaatcc gttacggctt
    48061 tctgaaaatc ttcagcggac cggcgagtat acctgaagaa aggacgttag atgcttttag
    48121 ctacggcact gttaattgtt ggtttacttt tggtcgttta cagtgccgac cgcctggttt
    48181 ttgccgcgtc tattctttgc cgaacctttg gcatcccgcc gctgatcatc ggcatgacgg
    48241 tggtcagtat tggtacatcg ttaccagaaa tcatcgtctc gcttgccgcg tctctgcacg
    48301 aacaacgcga tttagccgtt ggtacagccc tcggctcaaa cattatcaat atattgctga
    48361 tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca ttccgatgtt ctacgccgtg
    48421 aattaccctt aatgttgttg gtcagcgtgg tggccggttc cgtactctat gacggacaac
    48481 ttagtcgcag cgatggtatc tttctcctct ttctggctgt gctatggctg ctgttcattg
    48541 ttaaacttgc acgtcaggct gaacgtcagg ggactgacag cctgaccaga gagcagcttg
    48601 cagagctgcc gcgtgacggc ggattgcccg tcgcgttttt atggctcggc attgcgctta
    48661 tcatcatgcc agtggccacg cggatggtgg ttgataacgc cacggtgctg gcgaattact
    48721 ttgccatcag cgagttgacg atgggtctga cggcaattgc tatcggaacc agcctgccgg
    48781 aactggcaac cgcaatagcg ggcgttcgca aaggtgaaaa cgacattgct gtcggaaata
    48841 tcattggcgc aaacattttt aatattgtca tcgtgttggg tttacccgcg ctgataacgc
    48901 caggagagat tgatccactg gcgtacagtc gtgactacag cgtgatgttg ctggtgagca
    48961 ttatttttgc gttgctgtgc tggcggcgct ccccgcaacc gggccgtggt gtaggggtat
    49021 tattaactgg cggatttatc gtatggctgg cgatgttgta ctggttatcg ccaatactcg
    49081 ttgaataact ggaaacgcat tatgtcgcac gtagagttac aaccgggttt tgactttcag
    49141 caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc tggcggagct tgatcaatac
    49201 atcaatcaga atttcacgct tgcctgtgaa aagatgttct ggtgtaaagg gaaagttgtc
    49261 gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa tggccgcaac gtttgccagc
    49321 accggtacac cttcattttt cgtccatcct ggtgaagccg cgcatggtga tttaggcatg
    49381 gtcaccccac aggatgtggt gattgctatc tctaactcag gtgaatccag cgaaatcact
    49441 gccttaattc cagtgcttaa gcgtcttcac ataccgttaa tctgcatcac cggtcgcccg
    49501 gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg ttaaagtagc gaaagaagcc
    49561 tgtccgttag ggctggcacc gaccagcagc accaccgcca cgctggttat gggcgatgcc
    49621 ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg aagattttgc gctctcacac
    49681 ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa acgatattat gcatacgggc
    49741 gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg acgcattgct ggaagttacc
    49801 cgcaaaaatc ttggtatgac tgtcatttgc gatgacaata tgatgattga aggcatcttt
    49861 accgacggtg atttacgccg tgtcttcgat atgggcgtgg atgttcgtca gttaagtatt
    49921 gccgatgtga tgacgccggg gggaatacgc gtgcgccctg gcattctggc cgttgaggca
    49981 ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg ttgccgatgg cgaccattta
    50041 ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg tagtgtaaag attcaaggat
    50101 aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg ttacggccct gtcagcgccg
    50161 acgttatagc aaaagcagag aacattcgtc tgctaatcct cgacgttgat ggcgtactgt
    50221 cagatggcct gatttatatg ggcaataacg gcgaagagct gaaagcgttc aatgttcgtg
    50281 acggttatgg cattcgttgt gcgctcacct ctgatattga agtcgctatc attaccgggc
    50341 gaaaggctaa actggtagaa gatcgttgtg ccacattggg gatcactcac ttgtatcagg
    50401 ggcagtcaaa caaactgatc gcctttagcg atctgctgga aaaattggcg attgccccgg
    50461 aaaatgtggc ttatgtcggc gatgatctca tcgattggcc ggtaatggaa aaagtgggtt
    50521 taagcgtcgc cgtggccgat gcgcatccac tgttgatccc gcgcgccgat tacgtgacgc
    50581 gcattgctgg cggtcgtggc gcagtgcgag aagtttgcga cttattactc ctggcgcagg
    50641 gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa gccagacgtt gggttatcat
    50701 tgtgctatca ctggcggttc tggtgatgat cggtattaat atggccgaaa aagacgatac
    50761 cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa agcgagcata cggacacgct
    50821 cgtctataac ccagaagggg cgctaagcta tcgattgatt gctcaacacg ttgaatatta
    50881 ttccgatcag gccgtttcgt ggtttacgca gccggtactt accacgtttg ataaggataa
    50941 aatcccgaca tggtccgtaa aagcagataa agccaagctg accaatgacc ggatgctcta
    51001 tttatatgga cacgttgaag tcaacgcact tgtgccggac tctcaacttc gcagaatcac
    51061 gacggataac gcgcagatca atctggtgac gcaggatgtt acctctgaag acctcgtcac
    51121 gttatacgga acaacattta actccagcgg tctgaaaatg cgcggcaact tacgcagcaa
    51181 gaacgccgag ctgattgaaa aggttagaac atcctatgaa attcaaaaca aacaaactca
    51241 gccttaatct tgtgcttgcc agctcacttc tggccgccag cattccggca tttgccgtaa
    51301 ccggagacac tgatcagccg atacacattg aatcggacca gcaatctctt gatatgcaag
    51361 gcaacgtggt tacctttacc ggtaatgtca tcgtcaccca gggcaccata aaaattaatg
    51421 ccgacaaagt ggtcgttacc cgtccgggtg gcgaacaagg taaagaagtg attgacggct
    51481 acggtaaacc ggcaacgttc taccagatgc aggacaacgg taaacccgtt gaaggtcacg
    51541 cttcccagat gcactacgaa ctggcaaaag attttgtcgt tctgacgggt aatgcttatc
    51601 tgcagcaggt cgatagcaac attaagggcg ataagatcac ttacctggtg aaagagcaga
    51661 aaatgcaggc tttcagcgac aaaggcaagc gtgtaaccac cgttctggtg ccgtcgcagc
    51721 tgcaggacaa aaacaacaaa ggccagaccc cggcacagaa gaagggtaat taattcgtta
    51781 tggcaacatt aactgcaaag aaccttgcaa aagcctataa aggccgtcgc gtggtagaag
    51841 acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct gctggggcca aacggtgccg
    51901 gtaagaccac cactttctac atggttgtag gcattgtgcc gcgcgatgcg ggcaacatca
    51961 ttattgatga tgacgatatc agtctgctac ctctgcatgc acgcgcgcgc cgcggtatcg
    52021 gctatctgcc acaggaagcc tccattttcc gtcgcctcag cgtttacgat aacctgatgg
    52081 cggtactgca aattcgtgac gacttgtctg ctgaacagcg tgaagaccgc gcgaacgagc
    52141 tgatggaaga gtttcacatt gagcacctgc gtgacagcat ggggcagtca ctctccgggg
    52201 gtgaacgtcg ccgtgtagaa attgcccgcg cactggctgc gaatccgaaa tttattctgc
    52261 tcgacgaacc gtttgccggg gttgacccga tctcagttat cgacattaaa cgcatcattg
    52321 agcacctgcg cgacagcggc ttgggcgtgc tgatcactga ccacaacgtg cgtgaaacac
    52381 tggcggtttg tgaacgcgct tatatcgtca gtcaggggca tttgatcgcc cacggcacgc
    52441 ctacagaaat cttacaagac gaacacgtta agcgtgtata ccttggggaa gacttcagac
    52501 tctgataggg tagaagtttg cgacgtttta gcaggagagt acgattctga acatgaagca
    52561 aggtttgcaa ctcaggctta gccaacaact ggcgatgacg ccacagctcc aacaggcaat
    52621 tcgtctgttg cagttgtcga cgctggaact tcagcaggag ctacagcagg cgctggagag
    52681 taatccgctg cttgagcaaa tcgacactca tgaagaaatc gacacccgcg aaacgcaaga
    52741 cagtgaaacg ctggacaccg ccgacgcgct cgaacaaaaa gagatgccgg aagagctgcc
    52801 gctcgatgcc agttgggaca ccatttacac cgctggtaca ccatccggca ccagcggtga
    52861 ctacattgac gacgagctgc cggtctatca gggcgaaacg acgcagacct tgcaggatta
    52921 cctgatgtgg caggtcgagc tgacaccgtt ttccgacact gaccgcgcta ttgctacctc
    52981 tatcgtcgat gccgttgatg acaccggtta tctgactgtc ccgctggaag atattctcga
    53041 aagtatgggc gatgaagaga tcgacatcga cgaagttgaa gccgtcctta agcggatcca
    53101 acggtttgat ccggtcggtg tggcggcaaa agatctgcgt gactgcctgc tgatccaact
    53161 ctcccaattc gataaaacca cgccgtggct ggaagaggcc agactgatca ttagcgatca
    53221 tctcgatctg ttagccaatc acgacttccg cactttaatg cgcgtcacgc gtctgaaaga
    53281 agatgtgctg aaagaagccg tcaatctgat ccagtcgctc gatccgcgcc ccgggcaatc
    53341 gatccagact ggcgaacctg agtatgtcat tccagatgtg ctggtgcgta agcataacgg
    53401 tcactggacg gtagaactca acagtgacag cattcctcgt ctgcaaatca accagcacta
    53461 cgcctcgatg tgcaataacg cgcgcaatga tggtgacagc cagtttatcc gcagcaatct
    53521 gcaagatgcc aaatggttga tcaagagtct ggaaagccgt aacgatacgc tactgcgcgt
    53581 gagtcgctgt atcgttgaac agcagcaagc cttctttgag caaggcgaag aatatatgaa
    53641 accgatggta ctggccgata tcgcccaggc tgtcgaaatg catgaatcga cgatatctcg
    53701 cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt tttgaactga agtatttctt
    53761 ttccagtcac gtcaataccg agggcggcgg cgaagcttcc tccacggcga ttcgtgcgct
    53821 ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg ttgagcgaca gcaagttaac
    53881 ctctttgctg tcggaacaag gtatcatggt ggcacgccgc actgttgcga agtaccgaga
    53941 gtctttatcc attccgccgt caaaccagcg taaacagctc gtttgaccca accgataagg
    54001 aagacactat gcagctcaac attaccggaa ataacgtcga gatcaccgag gcactgcgcg
    54061 aatttgttac agccaaattt gccaaacttg agcaatattt tgaccgaatc aaccaggtct
    54121 atgttgttct gaaagtggag aaagtcaccc acacctcaga tgcaacactg catgtaaacg
    54181 gtggtgaaat tcatgccagc gcggaaggtc aggacatgta cgctgccatt gatggcttaa
    54241 ttgataagct ggcacgccag ttgaccaaac ataaagataa actgaaacaa cactaattgt
    54301 ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca caacgggcca tttgtacggt
    54361 taatgctccg agcctgttcc actgtttgag tgggcaggtt cttaggtgaa attatgacaa
    54421 ataatgatac aactctacag cttagcagtg ttcttaacag ggaatgtacg cgaagccgcg
    54481 tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag cgagctggcg gcgaaacaac
    54541 ttagcctacc gcctcaggtg gtttttgaag ctatcctgac gcgggaaaaa atgggcagta
    54601 ccggtatcgg caatggtatt gccattccgc atggcaaact ggaagaagat actctgcgtg
    54661 ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt cgatgccatc gacaaccaac
    54721 cggtggactt actttttgcc ctgctggtgc cggcagacca aactaaaacg cacctgcata
    54781 ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat ctgccgccgt ttacgcgcag
    54841 cccagagcga tgaagagctg tatcaaatca ttacggatac caaaggtact ccggatgaag
    54901 cgtagttatt cggtaatgtc tcttttagac gttgtgagga gaaacagtac atggtactga
    54961 tgatcgtcag cggacgttca ggttcaggta aatctgtcgc cctgcgtgcg ctggaagata
    55021 tgggttttta ctgcgtggat aaccttcccg tagtgttgtt acccgatctg gctcgaaccc
    55081 tggccgatcg cgagatttct gccgccgtca gcattgatgt tcgtaatatg ccggagtcac
    55141 cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc tttctcaccg caactactgt
    55201 tcctggatgc cgaccgtaat accttaattc gtcgttacag tgacacgcgc cgactgcatc
    55261 cgctttccag caaaaacctg tcgctggaaa gtgctatcga caaagaaagc gatctgctgg
    55321 agcctctgcg ttcgcgagcg gatctgattg tcgatacctc agaaatgtcc gttcacgagc
    55381 tggcagaaat gctgcgtacc cgtctgctgg gtaaacgtga acgcgaactg acaatggtct
    55441 ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc agattacgtc tttgacgtgc
    55501 gcttcttgcc gaacccgcac tgggatccga aactgcgtcc aatgacaggt cttgataagc
    55561 ctgtcgccgc gttcctcgac cgccacacag aagtacacaa ttttatctac cagacgcgaa
    55621 gctatcttga gctatggtta cctatgctgg aaaccaacaa ccgtagctac ttgacggtcg
    55681 ccattggttg taccggcggg aagcaccgtt cggtgtatat tgcagagcaa ctggcagact
    55741 acttccgctc gcgcggtaaa aacgtccagt cacgccatcg tacgctggaa aaacgtaaac
    55801 catgactgtc aagcaaactg ttgaaatcac aaacaagctg ggcatgcatg cccggcctgc
    55861 aatgaagctg tttgaattaa tgcagggttt tgacgctgaa gtgctgttac gtaatgacga
    55921 aggcaccgag gctgaagcca acagcgttat tgcgctgctg atgttggatt ctgccaaagg
    55981 acggcagatt gaagttgaag cgaccggtcc acaggaagag gaagcactgg ccgccgttat
    56041 cgccctcttt aattctggtt ttgatgaaga ttaatcttca tcactttgac atacaactcc
    56101 cttcaaactc cccccgacaa taagaaaatc acgtactgaa atcgttctca atcaacgtca
    56161 attgtacatt tcgtgcgctt tttactttca gaagaacctt aagaaaacct taagaagcat
    56221 tgtttaggtt tcgtttaagt taatcgacca tactggagat cgtcagaaaa tatttccagg
    56281 agatggcatg attcgcttat ctgaacaaag tcccctgggc accggaagac atcggaaatg
    56341 ttatgcgcat ccggaagatg cccaacgctg tatcaagatt gtctaccatc gtggcgatgg
    56401 tggcgataaa gagatccgtc gtgagttaaa gtactacgcg catcttggtc gccggttaaa
    56461 agactggagt ggaataccgc gctatcacgg taccgtcgag acggattgcg gaaccggtta
    56521 tgtttatgat gtgattgctg attttgacgg caagccctcc attacgctga ccgaatttgc
    56581 tgaacaatgc cgttacgaag aagacgttgc acaactgcgc caattactga aacagctgaa
    56641 gcgttatttg caggataacc gtatcgtgac gatgtcgctg aagccgcaga atatcctctg
    56701 tcaccgcatt agtgagtcag aggttatccc cgtcgtctgc gacaacattg gtgaaagcac
    56761 attgattcca ctggcgacct ggtcaaagtg gtgttgtctg cgtaagcaag agcggttgtg
    56821 gaaacgattt atcgcccaac cggcgctggc tattgccctg cagaaagatc tgcaacctcg
    56881 tgagagcaaa acgctggccc tcgcttcgcg cgaagcttaa tccagctggt gttgttgcat
    56941 aaacggctca ccgcctaact gatacatctg ccgtaaaatc cacgcctgac ggctgcgcac
    57001 gtagcccgat ggcgcggaga ctttgaaacg aagtggatta ggtaatacag ctgccagtaa
    57061 ggcagcttcc gatcgggtaa gtttgctcgc gggtttgtgg aaataacgtt gtgccgcagc
    57121 ttcgacgcca aacacaccgt cgccaaattc ggcgatattc aggtaaaccg tcaggatacg
    57181 ctttttgctc cagacagttt ctatccccag cgttaatccg gcttccagcc cttttcgaac
    57241 ccagctacgc ccatcccata aaaagagatt tttggctgtc tgttgagaaa tcgttgaagc
    57301 accgcgaata cggttttcat tgcgctcatt gtgcgccagg gctttctcaa tggaagcgac
    57361 atcaaagccc cagtgctcag gaaatgtctg atcttctgcg gcaatcaccg ccagtcccat
    57421 ccacggcgag atttgatcca tactgaccct gtcagaatgt gccacgtaac gaaaattacc
    57481 atgcagccag gcgctgacct gtcgctcgac cattaccgca gagaagggaa caggtgcaac
    57541 gctaaacaac gcgatgcccc cgccccagaa aacggcgagg acaaccatta accgcagaag
    57601 aaaacggcga acgaaactaa acaccgttaa gcggctttta ctcattcagc cagaaccagc
    57661 acgcgggaaa ccagcttatc aatgccgctc gccgcttctg caatgttctg cgccagcata
    57721 tatgccgggg tggtgacaat cttattgtct tcatcaacca cgatatcatc gacaggacac
    57781 ggcacatgct ccgcgcccat ctcttccagc acttctgcgg tatcgatatc agtaccgatg
    57841 gtcaaacgca gcgggaaatc gaaaattttc ggcagcatcg ccggggcaat acacataaaa
    57901 ccaagcggtt ttccggcctg atgcatcgct tgtgccagcg cctttaattc acggtcaacg
    57961 gtgcattcgc gaccaagact ggcaaaattg cttaaattct ttgccgcgcc aaaccccccc
    58021 ggcacaatca acgcatccag ttcagcggca tcggcctggg ccagaggacg gatttcaccg
    58081 cgcgttattc gtgccgcttc aatcagcaca ttgcgcgttt ccgtcatcgc ttcgccagtt
    58141 aaatgattga taacatcaac ctgctgctta tccggtgcaa agcagaccgc ctgcgcaccg
    58201 ctgcgtgaaa tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc gtcatagacg
    58261 ccgcatccgc tcagaattac gccaattttc ttcattatga tcatcctttc gcaattgact
    58321 gaaacacatt aattttttta ataaaaatgg tacgcatcac acatttaact gattcatgta
    58381 acaaatcatt taagttttgc tatcttaact gcgtgcggcc tgaaaaacag tgcagtgccc
    58441 ttgtaactca tcataataat ttacggcgca gccaagattt ccctggtgtt ggcgcagtat
    58501 tcgcgcaccc cggtctagcc ggggtcattt tttagtggct tttgccaccc acgctttcag
    58561 cacttctacg tcgtgacgcc actcttcttt catctcttca atccattcac cgacgttatc
    58621 ttcccaggcg ggaaggtcag gagactgaat ttgctgaccc agctgttgca gatggcgcaa
    58681 ccccactgac cccgccgcac ctttaatttt atgtccttcc tcaacaatgc cttttttatc
    58741 ctgcgccgtc agattcgact ccagcacgtt gacatagcct ggcatcatct tctcaaacac
    58801 cgctaacccg tcggtgatca gcttcggtcc tacaagttcg agatactgtt ccagcatggg
    58861 aatatcgagc aatgcttctg atttactgtt ctcttctgtc gtcaccgtac tctcctcatc
    58921 atcctgggta tcccagaatt tcttgatcat cgcggttagc gccggaacag aaagcggctt
    58981 actcagcaca tcatccattc cagcattgag gtactcttgt ttgtctttca gcacgttagc
    59041 ggttaaggcc accagcggcg gtaaatcctc tcgcggataa cgcttcgtca gttcacgaga
    59101 gatatccagc ccggtcatat ctggcaactg aatatccagc aacaccaggt cgtattcgcc
    59161 cggtttaaac atctccagcg ccgccttgcc ggtcatggcg acatcaacgc tgttacctaa
    59221 tttttccagc acagaacgcg cgacaatcac gttcagttca atgtcttcca ccagcagcac
    59281 attcagcgcc ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct cttctgctac
    59341 cgacggtgcg tggatcgtca acgtaaaggt tgaaccttta ccctgttcgc tggtaaccgt
    59401 aatatcgccg cccatatttt tcgccagacg acgagaaacg gccagaccaa taccggtgcc
    59461 ggtggcaggt ttaccgccat gactgtcttt cacctggtaa tacatggcga agattttatc
    59521 cagctcatcc tgcggaatgc cgataccaga atcttccact tcaaaatgca gcatatcgcc
    59581 ttcatcgtag cgcacgcgca cggtaacctg gccttgctgg gtgaatttga cggcgttact
    59641 gatgaggttc cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct gatgcggtaa
    59701 tggcagcgtc ggctccaggt taaagcgcaa tcctttttgt tgcgcctgca atgcggagag
    59761 attttccaga tcggcaagga agctggtgaa atcaaccggt tgattatcaa gctggacctt
    59821 gcgccgttcc atcttatcca tgtcgataat atcgttaaag atattcccca gcgtgacggc
    59881 cgaaacatgg atggtcttga gatatttttc ctgctcggcg gtgagttcgg tatccagcag
    59941 aatgcggctc aggccgacga taccgttcag cggtgtacgc aattcgtggc tgatggtgga
    60001 aataaacgtc gttttgtcgc ggctggcgcg ttcaagcgca tcctgatacc gcttacgctc
    60061 ggtaatgtcg cgaccaaagc ccatcaaacc gtgacgttta cccacgcggt cgtagtacgg
    60121 cactttacga atttcaaagc aggctttgcg tccgtcggga taatccagcc actgctcata
    60181 ggtcagtgac acattatgac ggaacacttt ttcatcggtt tcaatgactt tcgcagcggc
    60241 ttccggtgag taaacatcag caggtttcag gtgaaccagt tgtttttcgc tttttccggt
    60301 cagcagctcc atcgcgcggt tacagccgga aaactcttta tcttcgttac gataaaaaac
    60361 caggtcgggt gaagcatcaa ggaaggaacg taaaaatgag gattgctgct cgagctgaat
    60421 ttgtgtctct tcgcgctctt tgatttcaat tttcagttgg ccgaaggttt cctgcagttc
    60481 tgcttccgct ttttcacgaa cggcaatttc ctgatttagc tgggcaatat tatcttttaa
    60541 ctgaacgttg aggctcaaat cgcgctcgcg catctcctcc agtttttgca ccagccgtga
    60601 cagacgttgt cgtgactcct ccagttgctc gacgaccacc gatagaaagt agaccgccca
    60661 cggcgtaatc agcaaaccaa agaagataga acgaataaca tcaatgcttt cgacctgacc
    60721 atgcagcacc atggttaccg ccatttgtac cacaatggca agaacgacga gggccagcgc
    60781 cagcaacatt gagaagcgca ccagacctaa cttcatcatc aggtcaacat aatactgcgc
    60841 cagcagacga atttgcttca ttagggaatt ccttcacgac aacctgcccc aataataccc
    60901 aattctgaca gttacgttat aaattgtgtg agaaatgcgg aattcccctc aacgacctac
    60961 tccgttggag gtagccacgg acgtcccagc gccgatccct gtacgccatg ttcattcaga
    61021 tagcgatcca gctcgaccat tcccgtccat cgattttcgc accacagagg agcaagcagc
    61081 gtcgggcgac gggcgctggc ggaaatgcgg tggtagatca cttccggtgg cgtatggcga
    61141 atcatttctc cggcagtgag cgtgtaatcc tccagttcaa taccgttcaa acgccccgct
    61201 tcccaggctt tcgccataat gctgcctttc acaatatgca gcggatgcag ctttatgccg
    61261 tccacgccgg tttcaaccac tcgctccagc gtttgcaaac attcagcctg cccttcgcca
    61321 ggcaggccga caattaagtg agaacacacc ttcagcccgc gctgacgtgc cagctgggtt
    61381 gtacgctgat aacaggcaaa atcatggccg cgattaatgc gatgcagggt tttgtcgtgg
    61441 gcggtttgta gccccagctc cagccacact tcgtagccct ggtccttata ttcgcaaagc
    61501 agatccagca ccgcatccgg cacgcagtcc gggcgagtac caacacacaa accgacaata
    61561 ttggcctggc tcaccgcctg ctgatacata gaacgcagca cctgaacttc cgcaaaggtg
    61621 ctggtatacg cctgaaagta ggccagatag cgtttagcgc ggttaactaa attcgcctgg
    61681 tgcgccagtt gctcggcaat ggaacgatgt tgctgcgctt catcggcaaa tgaggcaaca
    61741 ttacagaatg tgcagccgcc acgcccgatg gtaccgtcac ggttagggca gctaaaaccg
    61801 ccgtgcagcg tcagcttatg caccttttgc ccataacgac gggtgagatc accaccaaac
    61861 atattgacta atttctgtaa ctgcataatc tgatagaccg cgccttgaaa agaggccaaa
    61921 gcctgccatt tttagctcaa ttcggcgatg acttggatca atcgtcccgg cctgctttta
    61981 tcaactgcat aaccaatcaa aattaccgaa atttcatgca taatcacata aatcactttt
    62041 gcttatcttg tgtcagattt ttttatctcc ttatggattt taggcaaaac agtagcatga
    62101 aacgtcatta ccaattaagg cagtataaaa tgctggtttt ctcgtcagtt caaggcagga
    62161 taagggttaa cacgccttta tgacagtcag gaattggctg tttctctaac gacgcccctt
    62221 ttagccttaa ttagaaaatc cattttaatt tcagtcaatt aataaagaat tttgcgctaa
    62281 agcacatttc tgtaccaata agcttgccat ttgacctgta tcagctttcc cgataagttg
    62341 gaaatccgct ggaagctttc tggatgagca gcctgctcat catatttatg cagtaattga
    62401 gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa caagggggcg aatgcgaggc
    62461 gcgcgtatga cacgcaaacc ccgtcgccac gctctttctg tgcccgtgcg cagcggttcg
    62521 gaagtggggt tcccgcagag cctgggggag gttcacgata tgttgtacga taaatccctt
    62581 gagagggata actgtggttt cggcctgatc gcccacatag aaggcgaacc tagccacaag
    62641 gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc accgtggcgc gattctcgcc
    62701 gatggtaaaa ccggtgacgg ttgcggcttg ctgttacaaa aaccggatcg cttttttcgc
    62761 atcgttgcgc aggagcgcgg ctggcgttta gcgaaaaact acgccgtcgg gatgctcttc
    62821 ctgaataaag atcctgaact cgccgctgcc gcacgccgca tcgttgaaga agagctgcaa
    62881 cgcgaaacct tgtcgattgt gggctggcgt gatgtcccca ctaacgaagg cgtgctgggt
    62941 gaaatcgccc tctcctctct gccacgcatt gagcaaattt ttgtgaacgc cccggcaggc
    63001 tggcgtccgc gcgatatgga gcgccgtctg tttatcgccc gccgccgcat tgaaaagcgt
    63061 ctcgaagccg acaaagactt ctacgtctgt agcctgtcga atctggtgaa catctataaa
    63121 ggtctgtgta tgccggcgga tctgccgcgc ttttatctag atcttgcgga cctgcgtctg
    63181 gaatcggcca tttgcctgtt ccaccagcgc ttctccacta acaccgtacc gcgctggccg
    63241 ctggcgcaac cgttccgcta cctggcgcat aacggtgaaa tcaacaccat caccggtaac
    63301 cgccaatggg cgcgtgcgcg tacttataaa ttccagacac cgcttatccc tgacctgcac
    63361 gacgccgcac cgttcgtcaa cgaaatcggc tctgactcca gttcgatgga taacatgctg
    63421 gaactgctgc tggcaggcgg gatggatatc atccgcgcca tgcgtctatt agtaccaccc
    63481 gcctggcaga acaacccgga tatggacccg gaactgcgtg ccttctttga ctttaactcc
    63541 atgcatatgg agccgtggga tggcccggcg ggcatcgtga tgtctgacgg tcgttttgct
    63601 gcctgtaacc tcgaccgtaa cggtctgcgt ccggcgcgct acgtcatcac caaagataag
    63661 ctcatcacct gcgcctctga agtcggtatc tgggattacc agcctgacga agtggtcgaa
    63721 aaaggccgcg tcgggccagg cgaactgatg gtaatcgaca ctcgcagtgg gcgtattctg
    63781 cactcggcag aaaccgatga cgatctgaaa agccgccacc cttataaaga gtggatggag
    63841 aaaaacgtcc gccgactggt accgtttgaa gatctgcccg atgaagaagt gggtagccgc
    63901 gaactggacg acgacacgct tgccagctac cagaaacagt ttaactacag cgcggaagag
    63961 ctggactccg taattcgcgt actgggcgaa aacggtcagg aagcggtcgg ttcgatgggc
    64021 gatgataccc cattcgccgt gctctccagt cagccgcgca ttatttacga ctacttccgc
    64081 cagcagtttg cccaggtgac taacccgcca atcgacccgc tgcgtgaagc gcatgttatg
    64141 tcgctcgcca ccagtatcgg tcgtgaaatg aacgtctttt gcgaagcaga gggccaggcg
    64201 caccgtttaa gctttaaatc gccgattctg ctctactccg atttcaaaca gctcacgacg
    64261 atgaaagagg agcactaccg cgcagatacg ctggatatca cctttgacgt caccaaaacc
    64321 acgctcgaag cgacggtcaa agagctgtgc gacaaagccg aaaaaatggt acgtagcggc
    64381 accgtgctgc tggtgctctc cgaccggaat atcgctaaag atcgcctgcc ggttccggct
    64441 ccaatggcgg ttggcgcgat ccagacccgt ctggtcgatc aaagcctgcg ttgcgatgcc
    64501 aacatcatcg tcgaaaccgc cagcgcccgc gatccgcacc acttcgccgt gttgctgggt
    64561 ttcggcgcga cggctattta tccgtacctt gcctatgaaa cgctgggccg cctggtagac
    64621 acccacgcga ttgccaaaga ttatcgtacc gtgatgctca actaccgtaa cggcatcaac
    64681 aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca ccatcgcctc ttaccgctgc
    64741 tcgaaactgt ttgaagcggt cggtctgcac aatgatgtag tgggcctgtg cttccagggg
    64801 gcggtcagcc gcattggggg ggcgagcttt gaagacttcc agcaggatct gctgaatctc
    64861 tcgaaacgag cctggctggc gcgcaagccc atcagccagg gcggcctgct gaaatacgtc
    64921 cacggcggcg aataccacgc ctacaacccg gacgtggtgc gcacgctgca acaggcggta
    64981 cagagcggcg agtatagcga ctatcaggaa tacgcgaagc tggttaatga gcgtccggca
    65041 accacgctgc gcgatctgct ggcaattacg ccgggtgaaa acgcggtcaa cattgccgat
    65101 gttgaaccgg caagcgaact gtttaaacgc ttcgataccg cggcgatgtc tatcggcgcg
    65161 ttaagcccgg aagcccacga ggcgctggcg gaagcgatga acagcatcgg cggaaactcg
    65221 aactccggtg aaggtggtga agacccggcg cgctacggca ccaacaaagt gtcgcgcatc
    65281 aagcaggtgg cttccggtcg ctttggggtt actccggcgt atctggtcaa tgccgacgtc
    65341 attcagatta aagtcgccca gggcgcgaag ccgggcgaag gcggtcagtt gccgggcgac
    65401 aaagtcactc cttacattgc caaactgcgc tattcggtgc cgggcgtgac gctgatctcc
    65461 ccgccgccgc accacgatat ctactctatc gaagacttag cgcagctcat tttcgacctc
    65521 aagcaggtta acccgaaagc gatgatctcc gtgaagctgg tttccgaacc gggcgttggc
    65581 accatcgcca ccggtgtggc gaaagcttat gccgatttaa tcaccatcgc aggctatgac
    65641 ggcggcaccg gcgcaagtcc gctttcatcg gtgaaatacg caggctgtcc gtgggagctg
    65701 ggacttgttg aaacacaaca ggcgctggtt gccaacggct tgcgtcataa aatccgtttg
    65761 caggtcgatg gcggcctgaa aacgggtgtc gatatcatca aagcggcgat tctcggcgca
    65821 gaaagcttcg gcttcggcac tggcccaatg gtcgcgctcg gctgtaaata tctgcgtatt
    65881 tgccacctga acaactgcgc aacgggtgtg gcaactcagg atgacaaact gcgtaagaat
    65941 cactatcacg gcctgccgtt caaggtgacg aattactttg agtttatcgc ccgtgaaacc
    66001 cgcgagctga tggcacagct tggcgtaaca cgtctggtgg atctgattgg tcgcaccgac
    66061 ctgctgaaag agctggacgg tttcaccgcc aaacagcaga aactggcgct gtcgaagctg
    66121 ctggagaccg ccgaaccgca tccaggtaag gcactctact gcaccgaaac caacccgccg
    66181 tttgataacg gcctgctgaa cgcgcagttg ctgcaacagg cgaaaccgtt tgtcgatgag
    66241 cgccagagca aaaccttctg gttcgatatc cgcaacaccg accgctctgt cggcgcgtcg
    66301 ctttcaggct atatcgccca gacgcacggc gatcaggggc tggcagccga tccgattaaa
    66361 gcgtacttca acggcaccgc aggccagagc ttcggcgtgt ggaacgcggg cggcgtggaa
    66421 ctgtacctga ccggtgatgc caacgactat gtcggtaaag gcatggcggg cggcttaatc
    66481 gccattcgtc ctccggttgg ttccgccttc cgcagccatg aagcaagtat tatcggcaac
    66541 acctgcctgt atggagcgac gggtggtcgt ctgtatgccg caggccgcgc gggtgaacgt
    66601 ttcggcgtgc gtaactccgg tgcaatcacc gtggtagaag gcattggcga caacggctgt
    66661 gaatatatga cgggcggtat cgtctgtatt ctgggtaaaa ccggcgttaa cttcggtgcg
    66721 ggtatgaccg gcggcttcgc ttacgttctc gatgaaagcg gcgatttccg caaacgcgtt
    66781 aacccggaac tggtggaagt attaagcgtt gacgatttgg cgatccatga agagcatctg
    66841 cgtggtctta tcaccgagca tgtgcagcat accggttccc agcgtggtga agagattctg
    66901 gcgaactggt caaccttcgc cactaaattt gcgctggtta aaccgaagtc cagtgatgtc
    66961 aaagcactgc tgggtcatcg tagtcgtagc gcagcagagt tgcgcgtgca ggcgcagtaa
    67021 ggggtagcaa caatgagtca gaatgtttat caatttatcg acctgcagcg cgttgatccg
    67081 ccaaagaaac cgctgaagat ccgcaaaatt gagtttgttg aaatttacga gccgttttcc
    67141 gaaggccagg ccaaagcgca ggctgaccgc tgcctgtcgt gcggcaaccc atactgcgag
    67201 tggaaatgcc cggtacacaa ctacatcccg aactggctga agctcgccaa cgaggggcgt
    67261 atttttgaag cggcagaact gtcgcaccag accaacactc tgccggaagt atgcggacgt
    67321 gtctgcccgc aagaccgtct gtgcgaaggt tcctgcaccc tgaacgatga gtttggcgcg
    67381 gtgaccatcg gcaacattga gcgttatatc aatgataaag cgttcgaaat gggctggcgt
    67441 ccggatatgt ccggcgtgaa acagaccggt aaaaaagtgg cgattatcgg cgcaggtccg
    67501 gcaggtctgg cgtgtgcaga tgtcctgacg cgcaacggcg tacaagcggt cgtcttcgac
    67561 cgtcacccgg aaattggcgg cttgctgacc ttcggtattc cggctttcaa actggaaaaa
    67621 gaggtaatga cgcgccgccg tgaaatcttc accggcatgg gtattgaatt caaactcaat
    67681 accgaagtgg gccgcgacgt acagctggac gatctgctga gtgattacga tgccgtgttc
    67741 cttggcgtcg ggacttatca gtcaatgcgc ggcgggctgg aaaacgaaga cgccgatggc
    67801 gtgtacgcag cgctgccgtt ccttatcgcc aacaccaaac agttaatggg ctttggcgaa
    67861 actcgtgaag aaccgttcgt cagcatggaa ggcaaacgcg tggtagtact tggcggtggc
    67921 gataccgcaa tggactgcgt gcgcacgtct gtacgccagg gggcaaagca cgttacctgt
    67981 gcctatcgtc gtgatgaaga gaacatgccg ggttcccgcc gcgaagtgaa aaacgcgcgg
    68041 gaagaaggtg tagagttcaa attcaatgtc cagccactgg gaattgaagt gaacggtaac
    68101 ggcaaagtca gcggcgtaaa aatggtgcgt actgaaatgg gcgaaccgga tgccaaaggc
    68161 cgtcgccgcg cggagatcgt ggcaggttcc gaacatatcg ttccagcaga tgcggtgatc
    68221 atggcgtttg gtttccgtcc acacagcatg gaatggctgg caaaacacag cgtcgagctg
    68281 gattcgcaag gccgcatcat cgccccggaa ggcagcgaca acgctttcca gaccagcaac
    68341 ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg atctggtcgt taccgctatt
    68401 gccgaaggtc gtaaggcggc agacggcatt atgaactggc tggaagttta agctctgtcc
    68461 cagcgccgcc tcctcagaaa agagtcggcg ctgaaattct cctttcgttc tggaaagcgc
    68521 ctcgcataat ctgaagaaaa ccaatgtaat acgcacatct ggattgttac cggcagatgc
    68581 ccggcgtata gctgattcca ttattcaaca ctgcgccata taaagggaaa gataatggaa
    68641 tcgctctcag agagaacctc aacaggctac cagcaaatcc acgacggtat tattcacctg
    68701 gtcgatagcg cccggacgga aacggtacgt agcgttaacg cgctgatgac cgcgacatac
    68761 tgggaaattg gccgacgaat tgtcgaattt gaacaaggtg gcgaggccag ggctgcgtat
    68821 ggtgcgcagc taatcaagcg actatcaaag gatttaagtc taaggtataa gcgtgggttc
    68881 tctgcaaaaa acttacgcca aatgaggctt ttttacctct tttttcaaca tgttgaaatt
    68941 cgccagacag tgtctggcga attaacacca ttgccctggt ccacttacgt ccgtttactt
    69001 tccgttaaaa acgctgacac ccgcagcttt tatgaaaaag agacgctccg ctgtggctgg
    69061 tctgttcgcc agctagaacg gcaaattgcg acccaatttt atgagcgaac actgctgtca
    69121 catgacaaat cagccatgct gcaacaacac gctcctgccg agacgcatat tcttccgcaa
    69181 caggcgatac gcgatccctt tgtgctcgaa tttctggaat tgaaagatga atactcagaa
    69241 tccgattttg aggaggcgct gatcaaccac ctgatggatt tcatgctgga acttggggat
    69301 gattttgcct ttgttggtcg gcagcgaagg ttacgcattg atgacaactg gtttcgtgtc
    69361 gatctgctgt ttttccaccg ccgtttacgc tgcctgctaa tcgtcgatct aaaagtgggc
    69421 aaattcagct atagcgatgc cggacagatg aatatgtatc tcaactacgc caaagagcac
    69481 tggacgctac cggatgaaaa tccgcccatc ggtctggttc tctgtgcaga gaaaggagtc
    69541 ggagaagcgc attatgcact ggctggtttg cctaacaccg ttctggcgag tgaatataag
    69601 atgcaactac ctgatgaaaa acgactcgcg gatgaactcg ttcgaacaca ggcggtgcta
    69661 gaagaaggct atagactccg ttaatttcag cggtttcgaa ttctgcagac actgtctgca
    69721 gaatctttaa aacgaactaa gttttaagcc attaaatcag ccttaacctt caccaccacc
    69781 tttttaatct caccaggttc gccgacaacg catcctggtt tatgcggttc tcccggcata
    69841 aacacggcga acattcccgg ttttaagttg atggtttgct cgttttcaat ggcgctgcaa
    69901 agctggtaat catcttcatg gtggaactct tcacactggc gcgcagtgcc tgccgtgcca
    69961 aacagaatcc gttcctcacc ttttaataac agctggatat caatgtattg ctcgtgcaat
    70021 tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat aaaaatattg
    70081 tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc taatgccagc
    70141 gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg tacttcaccc
    70201 atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct gcatcatggc
    70261 ggtagtgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc agatacgttt
    70321 ccaccagcgc cagataccct tctgccagac caacgctgcc accgaccacc acgcactgac
    70381 aatcagttgt ggctttaata tcagcgatca gccttgccag cacatgtgcg gagcggtgaa
    70441 tcagctgctg cgcctgctcg tcgccctgcc cggcgcgcat gaaaatagtt ttcgcatccg
    70501 cgccagccaa ctccccctgc gctgccgctg caataccgcg accagaggca atcgcttcca
    70561 cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc gtatgcccga
    70621 tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact acaccgccgc
    70681 cgacgccagt ggaaacggtg ataaacacca tatcggttat atcgccttcc agcgcctgat
    70741 actctgccca tgctgcggcc tgggcgtcgt taatggcaat ggtcggcaaa tcggtaagtt
    70801 gccccagcgt tttgactaac ggaaagtgta gcaatccacc aagattatgc ggattcagcg
    70861 ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc tgcgcatgag
    70921 cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt gtctggctgg
    70981 ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc agcgcggcgg
    71041 caagtttagt accgccgata tcaatcgcca gtgtggtcat agcaccgcct ttttcatcgc
    71101 tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga ccgtcaccgc
    71161 ccacgcgccg tggcgcatcg catcagccgc ctgagcaggc gtgttgtaac gcccttcggc
    71221 aatcacccga catccggcgt cgctcaacct tttcaccagc gtcagatccg gctcttctgg
    71281 cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc ccagcttttg
    71341 gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac cgtgatgatg
    71401 aatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc cgtcaatggc
    71461 gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg ccgtgatgcg
    71521 taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc tcaccaccgc
    71581 acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc ccgcctgctc
    71641 tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt ccggaaccgg
    71701 ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt gtgcaagtaa
    71761 cgacatacat cttcccttag cgaaaggccc gatacagaga ccgggcaaca ggattaactt
    71821 ttggttttga ctaaatcgtt tttggcgctg ccaaacggca cggcaccgct aaatggtttg
    71881 ccgtcgatag cgtcatgagt acgcaacgct tccgggcgca gccaacgctg aacacgagaa
    71941 ggcatatcca gaccaatcag caggatcacc acaaatgtca gactgaacga gagcgatgcc
    72001 agtgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg ggccagtgcg
    72061 ccacccaatg cgccaacgtt ataggtaaag cccaggcccg ctgcacgctg gtcggtatcg
    72121 aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag catttgctgg
    72181 aagaacagta acagaccgag cacccagacg tttgcaccgc caatagcaaa taccggaata
    72241 atcagcagct gcgaggccaa cagactacaa acgtacgctt tgcgggttcc cagccagtca
    72301 ccgaggaagc caccgacgca gcaccccacc gccgcgccaa agccactaaa gaacagcaca
    72361 ttggcgacag tatgagggtt ataagccaga tcggttttca gatacgttgg cagcagcgcc
    72421 tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat tacgccagtt
    72481 ggccagcgtt tgcccgtact ctgcaccata aagctgataa agattgcggt gcataacagc
    72541 ccaagaacag cgacgatcgc ggcattttgc aggttaccgg cgaagcagaa ccacagcgca
    72601 gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg gtagagaata
    72661 tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc ttctgcttcc
    72721 gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat aaagaacagc
    72781 gcacgccagc cccagaccgg aaccaccagg ctgtagacct gagcggcaac gacggcccct
    72841 acagagaagc ctgaaatcag aaagccactg gctttgttac gcagatgttt tggccagctt
    72901 tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc gatgaccaga
    72961 cgagcgataa acatggtgat gtagcctggc gcaaagccgc atgccagcgt cccggccgag
    73021 aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc catagcgccg
    73081 agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact tgccgcctgc
    73141 accgtcgtca gcccgaattc accttgtact tcggtgagta ccagggcgat taaaacgaaa
    73201 tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcccgcca ttgtgcacga
    73261 ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag tctcccgcgg
    73321 tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt ttatttcccc
    73381 tcacccggta ggggcgagcg aggggaaaca actcacccgc gctcttgcat caactgctgg
    73441 gccagcgcct tcagttctgg cagatatttt tcatctaccg gaccaaacgg tttgcggcac
    73501 agcggcacag aaacgacatc catataatgc agaacagttt tcaggccgcg gaatacaccc
    73561 gttttgatca gtaaatcaat gactttgttg cattcagttt gcagtttctg cgcggtctgg
    73621 atatcgcctt ctttcagcgc cttaacgatc ccctgatagc gccagcccat gatgttgtag
    73681 gtactaccga taccaccatc agcgcccgcc agcaggccag aggcgaagat ttcgtcgtaa
    73741 ccgttataga gcaccagatc aggatgttca cgacggatct gctccatctg atagagatcg
    73801 ccagaggtct gtttcagcgc acctacgcca ggcaatgtaa caagtgtgtt gatctgatcc
    73861 agggtcagtt ttaccccact cagggctgga atgttgtaca ccaccatcgg caaaccatcc
    73921 gccgaatcaa taattgcccg atagtgatcg cagtgttctt caaagctgaa aggatagtag
    73981 aacggcgtga cggcggagac ggcatcgaag ccataacgtt tagccgatgc cgcaagttgt
    74041 tggctttcgg cggtgctgac gcaaccgacg tgggcgatga gtttaatctt acctttcgcc
    74101 tcttcggcga cgatttccag tacctgttca cgctcggaaa ggctttgtac aaaggcttcg
    74161 ccggtcgaac cacccacgta taaaccgtca atgccctgct gaatattgaa ctgaaccagg
    74221 cggcgcagac tcgctttatc cagtgcttgt tgttgatcaa aaggagtcag gagtgcagcc
    74281 attacgccac gtaaattcgt tgccataaat acctctgaag tgatgcttgt ctgataaacg
    74341 atataccttt atacctgtta taccagatca attaagcaac accccataca gaaagcttat
    74401 aatgcgatct gcttcactaa agtggcatta tttctttttg ttggtggtct gaccgaaggc
    74461 gtgccaggta gcagagacgc tgttgagatg cgattgcaac gcacgatcgg cttcgtcagg
    74521 atcgtggcgg cggatcgcgt caacgatcgc aatatgctgt tgatagctaa cgttgttatg
    74581 ctcgtgcaga gcctgatcag ccacggttgg acgtgcggcg atcagccagt cgagcagggc
    74641 aacgtggatc gccatgaaga ttgggttacc ggggatctcc gccagcacgc ggtggaaatc
    74701 aacgtctgag cgaatgaatg ccgcgttgtt atccagcgac tgactgttga tttccagtgc
    74761 ttttgccagc aaatcgattt gctcatcggt ggcgtgttca gccgcatagc gcaccaggct
    74821 ggattcaaag aacagacgta attgttcgaa atgggcaatc ccaccaggat gagaaaggaa
    74881 atctttcgcc atgccggaaa gctcgccgat gatggtgtcc gcagaaggac gtgagacgcg
    74941 ggcacgttcg ccgttgttta tttgcaccag acctttgcgt tttaacgctg ccagcgcttc
    75001 acgcaccgaa ggacgcccga cgttaaagaa cgccatcagt tcgcgttcag acggtaattg
    75061 ttcaccttcg ccaaattcac gacggcggat catctgttcc agctcttctt ccaccatttc
    75121 ggagagtttt ttacgcgcca gcgggcggct acgcaagttg cgaccaattg caggtgaaga
    75181 atcttcggtt tgcgaatcaa atgcgttcat aaggcccatt ctgtaaggtc agtgtgatta
    75241 acatcatcag tgacatccta tcacaggatt gaaagtaggg gaaaatggca gggttttctc
    75301 tttgtgcctc atcattacca taattaacgg aataattaac tattgcgaaa aattaatgta
    75361 acgcagataa aaacatcccg tttgaattat ttataagact attcacgagc attatgaata
    75421 ttatgaatgt gttcttacaa aataatcata agcgcatatt ttttaatgaa aaatcacctc
    75481 acctacaatt aaaaacacga catccgcacc ataaatagac ttgcaaaaaa tataacatca
    75541 ttgttttcaa tctgccgttt atgggattga cccttttctt ttgacacgga gttcaacaat
    75601 gttcggcata attatatctg tcatcgtatt aattacgatg ggctatttga tcctgaaaaa
    75661 ctacaaacct caggtggtgc tggctgcagg tatcttcctg atgatgtgcg gtgtctggtt
    75721 agggttaggt ggtgtactcg atcccgccaa aagcagcggc tacttgatcg tcgatattta
    75781 taatgaaatc ctgcgcatgc tgtccaaccg cattgccgga ttggggctgt cgattatggc
    75841 ggtgggcggt tatgcccgct acatggagcg cacaggagcc agtcgcgcga tggtgagctt
    75901 gttaagccgc ccgttaaaac tcattcgctc gccgtatatt attctgtcgg caacttacgt
    75961 catcggccaa atcatggcgc agtttattac cagcgcctcc ggcctgggta tgttgctgat
    76021 ggtcacctta tttccgacac tggtgagtct gggagtaagc cgtctctctg cggtggcggt
    76081 tatcgcaacc acaatgtcca ttgagtgggg gattctggaa acgaactcca tttttgcagc
    76141 ccaggtagcg ggaatgaaaa ttgccactta cttcttccac taccagcttc cggtcgcctc
    76201 ttgcgtcatt atctcggtgg cgatctccca ctttttcgtg caacgcgctt ttgacaaaaa
    76261 agataaaaat atcaatcacg aacaggcaga gcaaaaagct ctcgataatg tcccgccgct
    76321 ctattacgcc attttacctg tgatgccgtt aatcctgatg ctcggctcgc tattccttgc
    76381 ccacatcggg ctgatgcagt cagaactgca tctggtggtg gtgatgttac tgagtttgac
    76441 gatgtttgtt gagttcttcc gcaagcataa cttgcgcgaa acaatggacg atgtgcaggc
    76501 gttttttgac ggcatgggta cgcagtttgc caacgtggta acgctggtgg tcgcgggtga
    76561 aatatttgcg aaaggcttaa cgacgattgg cactgtcgat gcggttatca ggggtgcgga
    76621 gcattctggt ctgggcggta ttggcgtgat gattattatg gcgctggtca ttgccatttg
    76681 tgccattgtg atgggctctg gcaatgcgcc gtttatgtca tttgccagtc ttattccgaa
    76741 tatcgcagcc ggactacatg taccagcggt tgtaatgatt atgccgatgc attttgccac
    76801 gacgctagcg cgcggtttcg ccgattactg cggtggtggt cgttacgtca ggaattgcag
    76861 gcgtttcgcc ttttgcggtg gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg
    76921 tgaatatgat tgccacaatc acgctatttt attaagtcat taaaagacaa aacaggccgc
    76981 cagggcctgt ttttgtatta cttcacaacg cgtaatgccg gtcgaccacc gcgtggtggc
    77041 tgcggtggtt catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata
    77101 accgacataa cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct
    77161 tcatcgtagg cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata
    77221 gccagcacgg cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta
    77281 aagcgcacct catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt
    77341 acgatttgcc cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc
    77401 accaccaggt gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga
    77461 tagggacgac gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacgca
    77521 tttcacgttc tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca
    77581 tatagccttt cagctctttc gcacccgggc cgctgaactc gatacccagt tgcggcagac
    77641 gccacagcag cggagcaagg tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg
    77701 gcttctgacc gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg
    77761 catctgcttc agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt
    77821 cgatgcgatg catgtacaaa cggctttcac cgcgagctac cgggtaaaca ggcatcagtg
    77881 gcggatgcgg gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca
    77941 gctcacgatc caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag
    78001 gcggattgtc cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa
    78061 tgcggacctg atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt
    78121 tgttggcagc gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc
    78181 caccaggtgg ccagtcagaa gttgtgttac ccagtaagga acgactctct ttgttcgaaa
    78241 atcaaacaaa aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca
    78301 gattttgcga cctttgtggt gagtcgatgc cggaaatggg gaaaaagaga tgcgctttag
    78361 tctgaaatag ttgacttagt cccttattgg cgatgtggtt tttgttttgc ctgtctgtca
    78421 gacggcagca aaaagcaact ttccattttt tacgctgatt cagaatttaa ccataaaaaa
    78481 acccgccgaa gcgggttttt tcgcaaattg ttttctgccg gagcagaagc caattaacgt
    78541 ttggagaact gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga
    78601 cgagcgtcac gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat
    78661 tccatcagag cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca
    78721 cctttaacgg tgatgtacag gtccagtttc tcaaccatat cgaccagttc cagcggctga
    78781 cgaactacca tgcgagcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt
    78841 acgattttac cgttgcccgg tttgatgaaa acgcgagctg cggaactttt gcggcgacca
    78901 gtgccgtagt attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc
    78961 ggttgctgtg ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata
    79021 gcacgaccca gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca
    79081 ggacggcgag caatcatctc ttcaaaggtc gcttgtttga taccaccgat gtggccggtg
    79141 tggtgatagt acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc
    79201 agaacgatga tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg
    79261 cgcaggcgac gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca
    79321 acataccagt cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta
    79381 cccaataaat agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac
    79441 gacaaagtcc ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg
    79501 gaaaaaaact ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc
    79561 gacccctttt tgtgatttgt ggcaggtttt aacccgctaa atgctcgcgc ttcagatact
    79621 cttcgctttg catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat
    79681 cgccctgata aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct
    79741 cgtaaaactc atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca
    79801 taactggtac atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat
    79861 gctgactgcg ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga
    79921 cgcccattgt cgctaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcc
    79981 cccccgccag cgccaaccat agtttatcca tttgcgcccg ggtttcatcg tggagtggcg
    80041 aaagccacag atgcgcctga gtgagtgtac gcagacgata atcaacacca gcgtccacgt
    80101 tcattacatc acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca
    80161 agccatttcg ataaagttcg tccggcggaa tatttgacgt cgctaccagg gtaataccgc
    80221 gggcgaacag ggctttcatc aggccgccaa gtagcatggc atcggtaata tcagagacaa
    80281 aaaattcgtc aaaacagagc acgtcagttt ctgctttaaa acgatcagca ataatttcca
    80341 gcggatcggt ctgcccctgt aaggcagtta gctcttcatg cacccgcagc ataaaacggt
    80401 gaaagtgcag gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc
    80461 aggttttccc gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg
    80521 tgtcttcccg tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg
    80581 ctggtggcgt gctattgatg agttcctgat aaataatttc caagcggctg acggcctctt
    80641 tttgaacgtc gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg
    80701 gagtaacgct ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac
    80761 ggttgacgaa aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt
    80821 ggaattaacg gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct
    80881 acggaaacaa aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag
    80941 tcgtcggcat cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc
    81001 aacaggcgtt gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag
    81061 agctggttag ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc
    81121 gccagctgta tcagcacatg gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg
    81181 aagcaaaccc gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg
    81241 tgcagatgcc tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc
    81301 gcgactaatt tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact
    81361 atcatttaat ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt
    81421 tgaatctctt ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaacccagc
    81481 tgttgagtgc attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgttg
    81541 cgtcgatccc aggccaggtt gccgatcagg cccccctccc cagtctggct ccaatgctgg
    81601 aaaaagtgct tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga
    81661 aaatcccgga agaattcaaa aagttttttg gtgatgattt accggatcaa cctgcacaac
    81721 ccttcgaagg tttaggctcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca
    81781 acaaccatgt gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt
    81841 ttgatgcaaa actgattggt agcgatgacc agagcgatat cgccctgttg caaattcaaa
    81901 acccgagcaa attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt
    81961 ttgccgtggc ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt
    82021 ccgcattagg acgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg
    82081 cttccattaa ccgcggtaac tccggcggtg cactgttaaa ccttaacggt gagttaattg
    82141 gcatcaacac tgcaatcctt gcgcctggcg gcgggagcgt cggaattgga tttgccatcc
    82201 ccagtaatat ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg
    82261 gtttgttagg catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg
    82321 acgtgcagcg tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg
    82381 gcgtcaaaac gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg
    82441 agttgcgctc tcgtatcgcg accaccgagc cgggcacgaa agtgaagctt ggcctgctgc
    82501 gtaacggcaa accactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca
    82561 gcgctgaaat gatcacgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag
    82621 atggcggcaa aggtattaaa atcgatgaag ttgtcaaagg aagcccagct gctcaggctg
    82681 gcttgcaaaa agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg
    82741 aaatgcgtaa agtgctggcg gcaaaaccgg ccatcatcgc cctgcaaatt gtacgcggca
    82801 atgaaagcat ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg ctgacgtgtg
    82861 atgtccggtt aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat
    82921 gtttgtgaag ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt
    82981 tgccatgcct tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga
    83041 tgagacgcct gccagttata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt
    83101 ttacaaccgt ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc
    83161 cggtgtaatc atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc
    83221 cgatcagatc atcgtcgcct tacaggatgg acgtgtattt gaagcattgc tggtgggatc
    83281 tgactctcta accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat
    83341 tccaattaat gcacgtcgcg taccgcacat tggcgacgtg gtactggcga tcggtaaccc
    83401 gtacaacctc gggcagacca ttacccaggg gattattagt gctacgggtc gaatcggtct
    83461 gaacccgacc gggcggcaaa acttcctaca aaccgatgct tccattaacc acggtaactc
    83521 tggcggcgcg ctggtgaact cgctgggcga actgatgggc attaacacgc tgtcgtttga
    83581 taagagtaac gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc
    83641 aaccaaaatt atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggtat
    83701 cggcggacgc gagatcgcac cactgcacgc gcagggcggt ggtatagatc aactgcaagg
    83761 gatcgtggtt aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa
    83821 cgatctgatt atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca
    83881 ggtggcagaa attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca
    83941 gttaacgctg caggtcacca ttcaggaata tccggcaacc aattaaggcc agcgtgaaag
    84001 acaaaaaaac cggagtctgt gctccggttt tttattatcc gctaatcaat tacttattaa
    84061 cgaactcttc gcccagggcg atatctttct tgagcgtatc cagcatacct tccagcgcgt
    84121 tctgttcaaa tgcgctcagg gtaccgatag atttacgctc ttccacgccg tttttaccca
    84181 gcagaagcgg ctgagagaag aaacgggcgt actgaccgtc gccttcaacg taggcacatt
    84241 cgacaacgcc ttgttcgccc tgcagtgcgc gaaccagaga caggccaaaa cgtgcagcag
    84301 cctggcccat agacagggtt gccgacccgc caccggcttt cgcttcaacc acctcagtac
    84361 ccgcgttctg gatacgtttg gtcagatcag ccacttcctg ctcggtaaaa ctaacgccag
    84421 gaacctgtga caacagcggc agaatggtaa caccagagtg accgccaata accggcactt
    84481 caacttcgcc tggctgtttg cctttcagtt ccgcaacaaa ggtgttggaa cgaatgatat
    84541 ccagcgtggt aacgccgaac agtttgtttt tgtcataaac accggctttt ttcagcactt
    84601 cagcagcaat cgcaactgtg gtgttaaccg ggttagtgat aataccaatg cacgctttcg
    84661 ggcaggtttt cgcaacttgc tgtaccaggt ttttcacgat gccggcgtta acattaaaca
    84721 ggtcggaacg atccataccc ggtttacgcg ctacacctgc agagataaga acgacatctg
    84781 cgccttccag cgccggagtc gcatcttcac cagaaaaacc tttgattttc acagcagtag
    84841 ggatatggct cagatcgacg gccacaccgg gagtcactgg agcgatatca tacagagaga
    84901 gttctgaacc tgaaggcagt tgggttttta acagtagtgc aagcgcctgg ccaataccgc
    84961 cagcagcgcc gaggactgcg actttcatcc taaactcctt attatattga taaactaaga
    85021 tatgttgctc cgctgccgcg accttaatcc acaaaattgc cgtttacaat gaccacatct
    85081 caagaatgtg tagtcacgca agtttagcgt ttatgcattt aattgccgta atcaggaacc
    85141 taagttacgt aattaacaat cgcttatgca ttagcgcaac attccaacag gtggtgacaa
    85201 tataccctac cgttcagcca aaacaacatc aatttgataa caattaattt acttttaagc
    85261 agaatttgca tgccgtgacg caggcatatt tctcaataac gaaatttgat aaaatcccgc
    85321 tctttcataa cattatttca gccttcttca gggctgactg tttgcataaa aattcatctg
    85381 tatgcacaat aatgttgtat caaccaccat atcgggtgac ttatgcgaag ctcggctaag
    85441 caagaagaac tagttaaagc atttaaagca ttacttaaag aagagaaatt tagctcccag
    85501 ggcgaaatcg tcgccgcgtt gcaggagcaa ggctttgaca atattaatca gtctaaagtc
    85561 tcgcggatgt tgaccaagtt tggtgctgta cgtacacgca atgccaaaat ggaaatggtt
    85621 tactgcctgc cagctgaact gggtgtacca accacctcca gtccattgaa aaatctggtg
    85681 ctggatatcg actacaacga tgcagttgtc gtgattcata ccagccctgg cgcggcgcag
    85741 ttaattgctc gcctgctgga ctcactgggc aaagcagaag gtattctggg caccatcgct
    85801 ggcgatgaca ccatctttac cacccctgct aacggtttca ccgtcaaaga cctgtatgaa
    85861 gcgattttag agctgttcga ccaggagctt taatctctgc cccgtcgctt ctggcggcgg
    85921 ggaaaatgtt gcttatccct ctcaaccccc tgctttcccc tgcgattaat ttaacgaata
    85981 gtgcatttta ctgcgacatg tcattcacac aatgaataca taaggtaaaa aaagcacatt
    86041 atgc
//
LOCUS       DAZKIF010000005        80286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZKIF010000005 DAZKIF010000000
VERSION     DAZKIF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.005"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(19..2331)
                     /gene="pbpC"
                     /locus_tag="ACHOTW_000373"
     CDS             complement(19..2331)
                     /gene="pbpC"
                     /locus_tag="ACHOTW_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP9842549.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
     gene            complement(2332..7293)
                     /gene="yfhM"
                     /locus_tag="ACHOTW_000374"
     CDS             complement(2332..7293)
                     /gene="yfhM"
                     /locus_tag="ACHOTW_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP9842550.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            7500..8345
                     /gene="sseA"
                     /locus_tag="ACHOTW_000375"
     CDS             7500..8345
                     /gene="sseA"
                     /locus_tag="ACHOTW_000375"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP9842551.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            8688..8813
                     /gene="timP"
                     /locus_tag="ACHOTW_000376"
     CDS             8688..8813
                     /gene="timP"
                     /locus_tag="ACHOTW_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP9842552.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(8845..9621)
                     /gene="sseB"
                     /locus_tag="ACHOTW_000377"
     CDS             complement(8845..9621)
                     /gene="sseB"
                     /locus_tag="ACHOTW_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP9842553.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRALLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9764..11047)
                     /gene="pepB"
                     /locus_tag="ACHOTW_000378"
     CDS             complement(9764..11047)
                     /gene="pepB"
                     /locus_tag="ACHOTW_000378"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP9842554.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11225..11425)
                     /gene="iscX"
                     /locus_tag="ACHOTW_000379"
     CDS             complement(11225..11425)
                     /gene="iscX"
                     /locus_tag="ACHOTW_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP9842555.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(11437..11772)
                     /gene="fdx"
                     /locus_tag="ACHOTW_000380"
     CDS             complement(11437..11772)
                     /gene="fdx"
                     /locus_tag="ACHOTW_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP9842556.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(11774..13624)
                     /gene="hscA"
                     /locus_tag="ACHOTW_000381"
     CDS             complement(11774..13624)
                     /gene="hscA"
                     /locus_tag="ACHOTW_000381"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP9842557.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(13641..14156)
                     /gene="hscB"
                     /locus_tag="ACHOTW_000382"
     CDS             complement(13641..14156)
                     /gene="hscB"
                     /locus_tag="ACHOTW_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP9842558.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14252..14575)
                     /gene="iscA"
                     /locus_tag="ACHOTW_000383"
     CDS             complement(14252..14575)
                     /gene="iscA"
                     /locus_tag="ACHOTW_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP9842559.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(14592..14978)
                     /gene="iscU"
                     /locus_tag="ACHOTW_000384"
     CDS             complement(14592..14978)
                     /gene="iscU"
                     /locus_tag="ACHOTW_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP9842560.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(15006..16220)
                     /gene="iscS"
                     /locus_tag="ACHOTW_000385"
     CDS             complement(15006..16220)
                     /gene="iscS"
                     /locus_tag="ACHOTW_000385"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9842561.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(16332..16820)
                     /gene="iscR"
                     /locus_tag="ACHOTW_000386"
     CDS             complement(16332..16820)
                     /gene="iscR"
                     /locus_tag="ACHOTW_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP9842562.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(17121..17858)
                     /gene="trmJ"
                     /locus_tag="ACHOTW_000387"
     CDS             complement(17121..17858)
                     /gene="trmJ"
                     /locus_tag="ACHOTW_000387"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP9842563.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            17977..18780
                     /gene="suhB"
                     /locus_tag="ACHOTW_000388"
     CDS             17977..18780
                     /gene="suhB"
                     /locus_tag="ACHOTW_000388"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP9842564.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            18925..19779
                     /gene="yfhR"
                     /locus_tag="ACHOTW_000389"
     CDS             18925..19779
                     /gene="yfhR"
                     /locus_tag="ACHOTW_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9842565.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            19970..21250
                     /gene="csiE"
                     /locus_tag="ACHOTW_000390"
     CDS             19970..21250
                     /gene="csiE"
                     /locus_tag="ACHOTW_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP9842566.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21242..22381)
                     /gene="hcaT"
                     /locus_tag="ACHOTW_000391"
     CDS             complement(21242..22381)
                     /gene="hcaT"
                     /locus_tag="ACHOTW_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP9842567.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(22541..23431)
                     /gene="hcaR"
                     /locus_tag="ACHOTW_000392"
     CDS             complement(22541..23431)
                     /gene="hcaR"
                     /locus_tag="ACHOTW_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP9842568.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            23567..24928
                     /gene="hcaE"
                     /locus_tag="ACHOTW_000393"
     CDS             23567..24928
                     /gene="hcaE"
                     /locus_tag="ACHOTW_000393"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP9842569.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            24925..25443
                     /gene="hcaF"
                     /locus_tag="ACHOTW_000394"
     CDS             24925..25443
                     /gene="hcaF"
                     /locus_tag="ACHOTW_000394"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP9842570.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            25443..25763
                     /gene="hcaC"
                     /locus_tag="ACHOTW_000395"
     CDS             25443..25763
                     /gene="hcaC"
                     /locus_tag="ACHOTW_000395"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP9842571.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            25760..26572
                     /gene="hcaB"
                     /locus_tag="ACHOTW_000396"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             25760..26572
                     /gene="hcaB"
                     /locus_tag="ACHOTW_000396"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP9842572.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            26582..27784
                     /gene="hcaD"
                     /locus_tag="ACHOTW_000397"
     CDS             26582..27784
                     /gene="hcaD"
                     /locus_tag="ACHOTW_000397"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP9842573.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            27881..28303
                     /gene="yphA"
                     /locus_tag="ACHOTW_000398"
     CDS             27881..28303
                     /gene="yphA"
                     /locus_tag="ACHOTW_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9842574.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(28351..29223)
                     /gene="yphB"
                     /locus_tag="ACHOTW_000399"
     CDS             complement(28351..29223)
                     /gene="yphB"
                     /locus_tag="ACHOTW_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP9842575.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29235..30329)
                     /gene="yphC"
                     /locus_tag="ACHOTW_000400"
                     /pseudo
     CDS             complement(29235..30329)
                     /gene="yphC"
                     /locus_tag="ACHOTW_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(30362..31360)
                     /gene="yphD"
                     /locus_tag="ACHOTW_000401"
     CDS             complement(30362..31360)
                     /gene="yphD"
                     /locus_tag="ACHOTW_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9842576.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(31385..32896)
                     /gene="yphE"
                     /locus_tag="ACHOTW_000402"
     CDS             complement(31385..32896)
                     /gene="yphE"
                     /locus_tag="ACHOTW_000402"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9842577.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(32919..33902)
                     /gene="yphF"
                     /locus_tag="ACHOTW_000403"
     CDS             complement(32919..33902)
                     /gene="yphF"
                     /locus_tag="ACHOTW_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9842578.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(33999..37280)
                     /gene="yphG"
                     /locus_tag="ACHOTW_000404"
     CDS             complement(33999..37280)
                     /gene="yphG"
                     /locus_tag="ACHOTW_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP9842579.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDIAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37398..38591
                     /gene="yphH"
                     /locus_tag="ACHOTW_000405"
     CDS             37398..38591
                     /gene="yphH"
                     /locus_tag="ACHOTW_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9842580.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(38654..39907)
                     /gene="glyA"
                     /locus_tag="ACHOTW_000406"
     CDS             complement(38654..39907)
                     /gene="glyA"
                     /locus_tag="ACHOTW_000406"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9842581.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40235..41425
                     /gene="hmpA"
                     /locus_tag="ACHOTW_000407"
     CDS             40235..41425
                     /gene="hmpA"
                     /locus_tag="ACHOTW_000407"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP9842582.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41470..41808)
                     /gene="glnB"
                     /locus_tag="ACHOTW_000408"
     CDS             complement(41470..41808)
                     /gene="glnB"
                     /locus_tag="ACHOTW_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP9842583.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41869..43203)
                     /gene="glrR"
                     /locus_tag="ACHOTW_000409"
     CDS             complement(41869..43203)
                     /gene="glrR"
                     /locus_tag="ACHOTW_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP9842584.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(43193..43906)
                     /gene="qseG"
                     /locus_tag="ACHOTW_000410"
     CDS             complement(43193..43906)
                     /gene="qseG"
                     /locus_tag="ACHOTW_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP9842585.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(44071..45498)
                     /gene="qseE"
                     /locus_tag="ACHOTW_000411"
     CDS             complement(44071..45498)
                     /gene="qseE"
                     /locus_tag="ACHOTW_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP9842586.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(46074..49961)
                     /gene="purL"
                     /locus_tag="ACHOTW_000412"
                     /gene_synonym="purI"
     CDS             complement(46074..49961)
                     /gene="purL"
                     /locus_tag="ACHOTW_000412"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP9842587.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            50218..51774
                     /gene="mltF"
                     /locus_tag="ACHOTW_000413"
     CDS             50218..51774
                     /gene="mltF"
                     /locus_tag="ACHOTW_000413"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP9842588.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51771..52274)
                     /gene="tadA"
                     /locus_tag="ACHOTW_000414"
     CDS             complement(51771..52274)
                     /gene="tadA"
                     /locus_tag="ACHOTW_000414"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9842589.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(52332..52967)
                     /gene="yfhb"
                     /locus_tag="ACHOTW_000415"
     CDS             complement(52332..52967)
                     /gene="yfhb"
                     /locus_tag="ACHOTW_000415"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP9842590.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            53176..54024
                     /gene="yfhH"
                     /locus_tag="ACHOTW_000416"
     CDS             53176..54024
                     /gene="yfhH"
                     /locus_tag="ACHOTW_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9842591.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            54080..54340
                     /gene="yfhL"
                     /locus_tag="ACHOTW_000417"
     CDS             54080..54340
                     /gene="yfhL"
                     /locus_tag="ACHOTW_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP9842592.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(54534..54614)
                     /gene="shoB"
                     /locus_tag="ACHOTW_000418"
     CDS             complement(54534..54614)
                     /gene="shoB"
                     /locus_tag="ACHOTW_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP9842593.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(55036..55416)
                     /gene="acpS"
                     /locus_tag="ACHOTW_000419"
     CDS             complement(55036..55416)
                     /gene="acpS"
                     /locus_tag="ACHOTW_000419"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9842594.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(55416..56147)
                     /gene="pdxJ"
                     /locus_tag="ACHOTW_000420"
     CDS             complement(55416..56147)
                     /gene="pdxJ"
                     /locus_tag="ACHOTW_000420"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP9842595.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(56159..56887)
                     /gene="recO"
                     /locus_tag="ACHOTW_000421"
     CDS             complement(56159..56887)
                     /gene="recO"
                     /locus_tag="ACHOTW_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9842596.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(56899..57804)
                     /gene="era"
                     /locus_tag="ACHOTW_000422"
     CDS             complement(56899..57804)
                     /gene="era"
                     /locus_tag="ACHOTW_000422"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9842597.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(57801..58481)
                     /gene="rnc"
                     /locus_tag="ACHOTW_000423"
     CDS             complement(57801..58481)
                     /gene="rnc"
                     /locus_tag="ACHOTW_000423"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9842598.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(58754..59728)
                     /gene="lepB"
                     /locus_tag="ACHOTW_000424"
     CDS             complement(58754..59728)
                     /gene="lepB"
                     /locus_tag="ACHOTW_000424"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9842599.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(59744..61543)
                     /gene="lepA"
                     /locus_tag="ACHOTW_000425"
     CDS             complement(59744..61543)
                     /gene="lepA"
                     /locus_tag="ACHOTW_000425"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9842600.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(61741..62220)
                     /gene="rseC"
                     /locus_tag="ACHOTW_000426"
     CDS             complement(61741..62220)
                     /gene="rseC"
                     /locus_tag="ACHOTW_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP9842601.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(62217..63173)
                     /gene="rseB"
                     /locus_tag="ACHOTW_000427"
     CDS             complement(62217..63173)
                     /gene="rseB"
                     /locus_tag="ACHOTW_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP9842602.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(63173..63811)
                     /gene="rseA"
                     /locus_tag="ACHOTW_000428"
     CDS             complement(63173..63811)
                     /gene="rseA"
                     /locus_tag="ACHOTW_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP9842603.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(63844..64419)
                     /gene="rpoE"
                     /locus_tag="ACHOTW_000429"
     CDS             complement(63844..64419)
                     /gene="rpoE"
                     /locus_tag="ACHOTW_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP9842604.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(64416..64571)
                     /gene="rseD"
                     /locus_tag="ACHOTW_000430"
     CDS             complement(64416..64571)
                     /gene="rseD"
                     /locus_tag="ACHOTW_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP9842605.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            64827..66449
                     /gene="nadB"
                     /locus_tag="ACHOTW_000431"
     CDS             64827..66449
                     /gene="nadB"
                     /locus_tag="ACHOTW_000431"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP9842606.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(66434..67171)
                     /gene="trmN"
                     /locus_tag="ACHOTW_000432"
     CDS             complement(66434..67171)
                     /gene="trmN"
                     /locus_tag="ACHOTW_000432"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP9842607.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            67303..68637
                     /gene="srmB"
                     /locus_tag="ACHOTW_000433"
     CDS             67303..68637
                     /gene="srmB"
                     /locus_tag="ACHOTW_000433"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP9842608.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(68670..69551)
                     /gene="yfiE"
                     /locus_tag="ACHOTW_000434"
     CDS             complement(68670..69551)
                     /gene="yfiE"
                     /locus_tag="ACHOTW_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9842609.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            69654..70241
                     /gene="eamB"
                     /locus_tag="ACHOTW_000435"
     CDS             69654..70241
                     /gene="eamB"
                     /locus_tag="ACHOTW_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP9842610.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(70297..70680)
                     /gene="grcA"
                     /locus_tag="ACHOTW_000436"
     CDS             complement(70297..70680)
                     /gene="grcA"
                     /locus_tag="ACHOTW_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP9842611.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            70985..71674
                     /gene="ung"
                     /locus_tag="ACHOTW_000437"
     CDS             70985..71674
                     /gene="ung"
                     /locus_tag="ACHOTW_000437"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9842612.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(71722..72759)
                     /gene="yfiF"
                     /locus_tag="ACHOTW_000438"
     CDS             complement(71722..72759)
                     /gene="yfiF"
                     /locus_tag="ACHOTW_000438"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9842613.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            72966..73385
                     /gene="trxC"
                     /locus_tag="ACHOTW_000439"
     CDS             72966..73385
                     /gene="trxC"
                     /locus_tag="ACHOTW_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP9842614.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            73454..74152
                     /gene="tapT"
                     /locus_tag="ACHOTW_000440"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             73454..74152
                     /gene="tapT"
                     /locus_tag="ACHOTW_000440"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP9842615.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            74184..76844
                     /gene="pat"
                     /locus_tag="ACHOTW_000441"
     CDS             74184..76844
                     /gene="pat"
                     /locus_tag="ACHOTW_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP9842616.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            76958..78313
                     /gene="pssA"
                     /locus_tag="ACHOTW_000442"
     CDS             76958..78313
                     /gene="pssA"
                     /locus_tag="ACHOTW_000442"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP9842617.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVLRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            78359..78682
                     /gene="yfiM"
                     /locus_tag="ACHOTW_000443"
     CDS             78359..78682
                     /gene="yfiM"
                     /locus_tag="ACHOTW_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP9842618.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(78679..79977)
                     /gene="kgtP"
                     /locus_tag="ACHOTW_000444"
     CDS             complement(78679..79977)
                     /gene="kgtP"
                     /locus_tag="ACHOTW_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP9842619.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(80094..>80286)
                     /locus_tag="ACHOTW_000445"
     CDS             complement(80094..>80286)
                     /locus_tag="ACHOTW_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9842620.1"
                     /translation="KAILTDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
BASE COUNT        19096 a        21874 c        20218 g        19098 t
ORIGIN      
        1 ataaatattt tgtatatttc actgaaatga aaaatgtact gacgcgattt gccctacgtc
       61 atccatcacc agcaattgat aatcaccttt atccgtcaaa tgcagggtca cgttgcgccc
      121 gcgttcagtt aacggttcgc cattcagaaa ccaccagcgt tcacctgccc ctccacttga
      181 ttgcaacggc aaagtcgctt ccgctgcgcc cggtaaacgt ttaataatct cgccatcgcg
      241 aacgcctgtc agctgcagcg ggagctgggc atcgtgaccg tatggcgggc aaattgtcga
      301 ggccggtggt aagcgcacag cgcgacgttc tgatgcgggc aaccagggtt ccagcggtag
      361 cggccagaca tttatcattt cttgtcgcgc ttgcgggcaa tcagcggcaa cacgtttgcc
      421 attttcatcc agccagatgg ggaaacgaat gccattaatg ccttcctgct ccggcagtaa
      481 tagagttggc ggctgactcc cgtccagcag ccaggttgcc aggcggcggc gacagttacc
      541 gtcaccttcc ggcaaagact gtccgcccgg ccagcaaata acgccacgag tgactgagtt
      601 cgggcgcggg tcttccggca gattagcact gcgcgacagt aagatattat tgacctgatt
      661 caacaatggt acggcgctgg caaaaccaaa ctgaccaaca acgggcgtgc cgtccggtct
      721 gccagtccag atcccaatga catagcgagc gttaacccca atcgcccagg cgtcacgata
      781 gccatagctg gtgcccgttt tccatgccag tggggcgacg cgcggcaagg caccatccgg
      841 caagggtagc gcttcatcag ccataatccg gcgaatgatc cacgccgctc ccgacgacat
      901 taaaggccgt tcaagcagcg gatcgtcagg ctgtaagcgc aatttgcctg ccttgccgtg
      961 gcgagcaaac gcggtatacg ctgccgccat atcttccagt ttcgcaccag cgccgccgag
     1021 aatgagcgaa agattcggcg cagcaccgtt gggcaaatat aacggcaatc caacattgcg
     1081 taactttgcc gcaaaccgtt tcggtccata ggcttccagc acctgcacag caggtaaatt
     1141 cagcgagcgc accagcgcct cgctcatgct gatcgggcca tgaaaaccgc tgtcaaagtt
     1201 gccaggtcgg taatcaccgg tacgccgggg gacgtcttgc agcagtgatg ccgggtggat
     1261 caatccttca tccagcgcca gaccgtaaac aaacggtttg agcaccgatc ctggcgatcg
     1321 gatcgcattg accatatcaa catgaccaaa gcgtgaatca tcgttgagat caaccgaacc
     1381 cacccagccg cgaacacgca tatcggtatg atcaaccacg atcatcgcca gtgagctgcg
     1441 cggtggtaat cgtcctttcc agttttgcgc cagttcttcc agacgtcgtt gaagaccggc
     1501 atccagcgta gtaacgattt tgtcgctttt gcttttaccg agcatcatgc gcgaaaacag
     1561 cggtgccagt tgcggcattt gtcggggtgc cagccagatg ggttcttccc ttgactcttt
     1621 tacctgctca cgggaccaca caccttgcac ggccatccgt tcgagcactt tattacgcgc
     1681 gacttcggca cgctccggcc aacgatccgg gcgaagacgg ctgggcgctt gcggcaaaac
     1741 cgccagcatt gccgcctcgg aatagcttaa attcgcaggc gactttccga gataagccca
     1801 acttgccgca ccgatcccct gcaacgtacc gccaaacgga gcgcggttaa gatacaaggt
     1861 cagaatttca cgcttagaca gatgccattc cagttgcaac gcgcgccaga gctggcgaat
     1921 tttgccgcca aatgttttgg ggtgaggatc aagcagacga gcaacctgca tagtgagcgt
     1981 gctgccaccg gaaataaccc gtcccgaagt gagatcttgc catgctgcgc gcgccaccga
     2041 gaatggattc accccaggat gcttccagaa ccagcgatct tcataattga tcagcgcttc
     2101 aaggtaacgt ggagaaacat cttcgattgt tacaggataa cgccagatgc cgtcagcatc
     2161 ggcgaagcgc cagagcggcg taccatcctg cgccacgacc actcgtgcgg gattgacttc
     2221 atgcagcggt agaggccaga gtttatctgc tccccacgct gcaagaaaaa ggagaaatgg
     2281 cgcagctgcc agcgttatcc agcagccgcg tttggttaac aagcgaggca tttacggtct
     2341 gacaatcagc aaatcttcag ccgcgccggt cgcccgccat tggggaacat acattgattc
     2401 caccatcggt tgcggtacct gatacgttcc cggcgtcacc gcccgcgcca gatacaccag
     2461 cgttaccggt tggtattcat caacggcaac cgccgccaca aagcgatcgt cacggaactc
     2521 aatgtgctta atactcgcct gctgcatctg gttcagtaag ttctgcactt cgccaccact
     2581 ttgctccagg ctggcgctac cgttcgccag attctggttt tccagttcca gacccgcagg
     2641 cagcagatcc acgactaacg catccggcac gctgttacta gcttttacct gcaaccagac
     2701 cagcaccaga tcgccgctac gtaacgagtc cagcgatttg ctcttaccat cagtaccaag
     2761 aatatgacgc tcaatttgca gcacattgtt cgccggtaaa ggtgcggatt gcggataacc
     2821 gctggcatcc acacgcagcc ataacggctg ctcgccactg ttggtcacct gcaaggtggc
     2881 aagttgatcg ctattcagat tgctgttttg cgctttctcg cctgtcagcg gctcagctga
     2941 gaaagaggtt tgcgcctgcc atttacctgg taaatcctga atcgtacggg cagccaggaa
     3001 caacgcgtta ctttcctgcg tcgatagcca gcgttcacca aacgcctgct gcgaaagtgt
     3061 gttcagcagg gagtactgct catcgggtag cagtttgttc tcttccagca aggagagcat
     3121 caacgcgttg tcgcgcagtg gactaccgta atcacccagc catatccgct catcactatt
     3181 acgcggcgtt tttagcgcca gcgcaatcgc ttcttcatca cgcgtcgcat cacccatggt
     3241 tttcagcgca acgccaagtt gcagcagcgg taaaccagaa gcggcatctg cacgatgctc
     3301 ccagatttca cgcagcgcac ccagcggagc cttttgctga cgggccaaca ccagcgcagc
     3361 gtaagactgt acggcgaatt tactggcttt gagattatcc gcgtacggga tcgacatcat
     3421 gcccggatct tgtaaatagc gcagcagacg ctcattaccc cggttaatgg cgtctgtcgg
     3481 cacgctgtaa ccctgttcgc ctgcgcggac caggaaatcc atcacgtaag ccgtcagcca
     3541 gtactcttcg tcaccgtttt tatcccacag cgcaaagccg ccgttatcac gttgcatttg
     3601 cagcaaacgg gaaatgccga tatcgaccga tgcacggcgt ttctcatcac tgtcgccttt
     3661 gatgcccaac gcctgcagtt gggctgcgtt ggtataaagt gacgggaaca ggccgctggc
     3721 ggtttgctca agacagccgt acggatacgc ttttaactct ttgatataac gtgcgatgtt
     3781 cagcggtggt ttgccgctca acaacaattg cccttccagc gtaacaggcg agaagttttg
     3841 caatccatcc gccggaatcg cccatgtctc accaggctgt aacgccgtac cgtaattaac
     3901 cgtttgtgcc gggaacgccg gacggacgcc gattttccac tgcttatgct gatcggcaac
     3961 ggtttcaccc ggtaacgcta acccgctaat ggtggcctga atttctccat cgccataacc
     4021 cggcaatgct cgcaccggga taaacagcgt agtacgcaca cctggcgcta attcaacggc
     4081 tgcgggtgaa tcgctgacca gttcaagcaa accactggcg gtcagggcaa cgttcagttt
     4141 ttgcggttta tcggtaagat tagtgatatc cagcgtcaga cgcgagttat cgccactcgc
     4201 cataaagcgc ggcatgttca gttcagcaat caccggtgcg gcaactatca ctttgctttc
     4261 gttgctaccg aattcatctg ccgtccaggc ttgcgccatg acgcgcaatt caccgttaaa
     4321 atcgccaatc ggcagtgtaa ccgagccttc gccctgttcg ttgagcgtta ccggcagcgc
     4381 ctgctgcgcg acaatattga cgtgattgac cggcggttta ccaccacgtt tcagctcatc
     4441 accatcgcca ccgaaacgca gagctgccag acgcccctga ccttcaataa cctgaccgta
     4501 aatatcgtaa atgtctgcgc catagcgttt ctgaccaaag aacgcctgcc acggatctgg
     4561 cgtgacgtag tcagtaatat tcagcacacc actatcaacg gcagacacca gcacattcac
     4621 ctgtttaggc ttctcgccat ttttagtgct ggctttaatt ttcacggtta atggttgatt
     4681 gggacgcatt tttgctggtg tttccagcgc cagatcgagg cgacggtttt catcaccaag
     4741 cggcagatgc aacacaccaa ccgcgcgttt tggcgtcgcg gagcgagatt tatcgccagg
     4801 acgcaccacc agcgtactca aataaagatc atgacgattc caggttttat cgaccggaat
     4861 cgtcagatcc agcccttgag ccggaacatc aatctcttgc caccacagcg gcccttcact
     4921 ggactcgacc atcgcataac ctttacccgc cgttggcgcg gcgatatgca acttaatggt
     4981 atcgccaggg cgataaccgg ctttatccag tttcagcgtg acacggtcgg gtcgcactgc
     5041 gccgctaccg tcgctattat cctgccagct atagccagcc caaaaacgaa cactactgac
     5101 cgcttcattc ggcgctttga cttccagacg ataagcgccc cactctaccg ggaaactgac
     5161 cttgccggtt tcgtccgctt tcagatccag agtttgttca ttttcaatca gatctttttg
     5221 atcaaactgt gattgccagc cttcatcttc tgaccagttc cagtagtaat cgcggcgttc
     5281 gcgaatcagg cgcacctgca agcccgacac ggcttttttc acgccttgcg catcgctata
     5341 aacgatgtca aaagcggcgt tactgccttc atcaacaatg ggctgtttca ccgtgctgtc
     5401 agtgcgataa tcgtaaaccg atttcgaggc gaactgcgga cggatccccg gcaatgcatc
     5461 ggcaggccag atagcctgct cagcgcggcg cgtcaccggg cgaccgcccg attccagcaa
     5521 actgccctgg aaaataacct gcaatgggga atgcgtttcc ttccactggc tttctgtaga
     5581 aacttcgccg cgccctttat catccagcgt caactgaact tcatccagcg tgcgggaaag
     5641 gttttcggca gctatatcgc cgaattcaaa accaggtaag gctgacacag cctcacgcag
     5701 tgggcgcagg aaaagttgcc cttgcaaagt attaccatta gcaggcgcac cgtacaggta
     5761 gtatcccacc acggagaatt tcacttcatc attcggcgta agcggggttt tctcaccggt
     5821 cagattcagc gccatacgct ctggcataaa atcttcgacg tggaaatccc acatccgata
     5881 ctggttatcg cccgtgttag cgcgaatatg ccacataccg gttgccgcat tgctatcgag
     5941 tggccaggta aagtggtaga ggccattttc cggctgacta acgacgctcc tgagtacctg
     6001 cccatcgggt ttaatcacgt ctaacttgat gggttgattg ggcaacgctt taccgtctgc
     6061 atcacgcagc aaaccattga ggattaccgt ttcacccggg cgataaagat cgcgcgggcc
     6121 aaacatgaaa aactgtttgc tatagcctgg cgcgccagca atgttaaatt ctgctaagtc
     6181 cagcgccgga agttttaaat cgagtagcgt tgtctgaccg tctttacgtg ccagcaataa
     6241 tgcggcgttt ttatcatttt ccagctgcac atgcccctga gcgtcactgg ttgcctgagt
     6301 cagagtctgc cctttctcat ttaataaaga gacttcaatt ccttgctggg ccgcgccgtt
     6361 ttccagactt tgggtaaaga tatccagacg attgtgataa cggtgagctg aaacgccgat
     6421 atcacttaac gtaaacagcg tcgcgggatt actgtaatcg taacgtccag cctgattcat
     6481 cacagccaga tacacgcccg cctgctgaag cggtttgata tcgcccagcg gcagcaataa
     6541 tttttcacgg gtgttacgcg caggattgag atcaaaccgt ccggtgtaga ccagatccgc
     6601 catctgcagc agtttgtctg actgccagtt cgccagcgaa ttgcggtatt cccattggct
     6661 aatgaatgct ggcagagatt ctggcttaac gcggaagaag ttaacatcga cattattaac
     6721 gttgagcgcc attaccggca gcccttcaac gactttacca ggcagcagcg aaccacggct
     6781 ggcaaaaccg acactgggtt ggatgtcgcg ggtagttata gttttttcgt aatctttact
     6841 gaaggttgcg ttgttgagtg ctttgacctc cttgccaatt gtaacgatca aatcacgttt
     6901 cggttcgagg tggcgtaaac gcagctcttt aagattatct gatagctccc aggcaccatc
     6961 cactttgccg ctttttttat cgacgacatg aataacgcgt gagaaatcct gatccgggtc
     7021 gagagggatg gagaacgtca gcaccagcgt ggctgcacca tcaagttgga cttcagagag
     7081 atccagcaat gtcagcgcct taccggcact ctgttgggcc agtttttgtc gctctggcgc
     7141 ggagagtttt gctgaactcg cgttttctga agaggccgct ttagtaactt cagaaggcgc
     7201 atcttttttc accgctgttg gcgcgttatc gttgttgtcg caccctgcca gcgccagcat
     7261 tagcatgcag gcggctacgc gtaacttttt catttttcat ccctgccaca atggcccgtt
     7321 agcaacgtcg aataattatt atgcgtgaga atttacgtta tgtaattcag tatcacgccc
     7381 atacaatccg aattttagtg agaattgtgg ctcagacagc caaatcagcc tcttttgctt
     7441 gtcgcttttc actaaacatc cgacaattta gcctaacccc ggcaaaaatg gagatgccta
     7501 tgtccacgac atggtttgta ggagccgact ggctcgccga acatattgat gacccggaaa
     7561 ttcagattat cgatgcccgc atggcgtcgc ctggacagga agatcgtaac gttgctcagg
     7621 agtatctgaa tggacatatt cccggcgcag tgttttttga tatcgaagcg ctttctgatc
     7681 acacttcccc gcttccgcac atgctgccgc gcccggaaac gttcgccgtg gcgatgcgtg
     7741 aattaggcgt taaccaggat aagcacctga ttgtctatga cgaaggtaat cttttctcag
     7801 ccccacgagc atggtggatg ctgcgcacct ttggtgtaga gaaagtgtcg attctggggg
     7861 gtggacttgc aggctggcag cgcgatgatc tgttgttaga agaaggtgca gtagagctgc
     7921 cggaaggaga gtttaacgcc gcgtttaatc ctgaagcagt ggtgaaagta accgatgtgt
     7981 tattggcaag ccatgaaaat acggcgcaaa ttattgatgc ccgcccggct gcacgtttta
     8041 acgcagaagt tgatgaacct cgcccaggtt tacgtcgcgg acatattccc ggtgcattga
     8101 acgttccgtg gacggaactg gtgcgcgaag gcgaactaaa aacgaccgat gaactggatg
     8161 cgatattttt tggtcgcggc gtcagctatg acaaaccaat tatcgtcagc tgcggctctg
     8221 gtgtaacagc agccgtggtt ttgttggcac tcgcgacgct ggatgtgcca aacgtgaaac
     8281 tgtacgacgg cgcatggagt gaatggggcg ctcgggcaga tttaccggtt gagccattga
     8341 aataagcttt ttacaggcaa taaaaaaccg ccgaatttgg cggtttttta ttgctggtct
     8401 ggttcgcggc ctttccagca ggttgacttg tgttgccgga gcaacgcagg caccccacag
     8461 tgttgaaata ctctctttta tgcctggctg tgtcaagata ataaacagag taattattca
     8521 aacatgagag tattattctt aatggccctt ttcgccgtct cgcaaacggg cgctggcttt
     8581 aggaaagaat gttccatgac cgtaaataca ggcaccccac agtgcttgct atcgcggcaa
     8641 tatcgccagt ggtgctgtcg tgatgcggtc ttcgcatgga ccgcacaatg aagatacggt
     8701 gcttctgtat cgtacttatt gtttctggtg cgctgttaac cgaggtaaat aataaccgga
     8761 gtctctccgg cgacaattta ctggtgatta acaaccttca gagcagcagg taagcccgaa
     8821 tgccgccctt tggggcggca tattttagat tatccgattc tgtttaaagt cacgcaaaaa
     8881 accaccccag cgacgttcat agaatggcgc aatatgttcg gtaataaagt ggctaattcc
     8941 tttttcccct tttttcacct gacaaatatc gattggttca tcgccaggta atgtatcggt
     9001 cgctacactt cccgccacct gaataatttc ttcgatatca ccatcggctt caatgccaat
     9061 aagtaaatta ggctgtgcct cttcgttctc tttaattgaa caaataaaag cacgcttcac
     9121 aggcttaatg gttttaaata aggtggtgag tgaatcaatc atttgtgctg gcggctctgc
     9181 gacttccgat aatatcagcg attcaccgcc ttccaggatt tcctggctgc tcagcggatt
     9241 tccctcttca ccaatcaaca aactgatttc acgcggcata aattctttac cggtcggcag
     9301 tttggcatta aggaagagcg tttcgccaag ggtcatctca aacaatgtgc gaacgggcat
     9361 tacgacaaat gcctgttcgt cttcaaccgc ctgttgaagt gcttctaacg aggtaaaaaa
     9421 aggaatgacg ctggtgccgt cttctttttc ccagtgctgt aaatcaagcg cgctatcttc
     9481 aaccacagcc tcgccctgcg ccgccgtacc aggcacccag acggtggatt ccagtagagc
     9541 acggaaaaag gccgggcggt gcgccggttc ggttgctgct ttttccagca ggtcttcaag
     9601 ttcatttttt gtttcggaca taagaaccac aattcattca acgttcgggg cacaagtgcc
     9661 tgatgcgcca tacttatcag gcctacaagg gattcacaat ttgttgaatt tgcaatactt
     9721 tgtaggccgg ataaggcgtt tacgccgcat ccggcatatt agtttacgcc gttaacagat
     9781 tagcaatcgt gcgcacacca agtcccgtag cacccgcaga ccactgttca accggcgctt
     9841 tacggtaagt cgccgagcag tcgatatgca gccagccttg ctgatagttc tcaacaaagt
     9901 gcgacaggaa gcccgctgcc gtgctcgcgc ctgccggata cgccgcgctt ccggtattgt
     9961 tcagttcggc aaagttagac ggcagctggc tgcggtggaa ttccgccagc ggcagacgcc
    10021 agaatggttc gttctcttgt gaggcactcg ccagcaaacg accggcaagc gcatcgtcaa
    10081 aactgaacag cgcgtgataa tcattaccca gcgcagtttt cgccgccccg gtgagggtcg
    10141 ccgcatcaat gatcatttcc ggtttctgcg cactggcatc aatcagacca tcggcaagca
    10201 ccaggcgccc ttcggcatca gtgttcatca cttcaacttt tttaccgttg cgataagtga
    10261 tgatatcgcc cagcttgaac gcattgccgc taatcaggtt atccgcacag cagaggaaca
    10321 gcttcacgcg cttgttcagt ccgcgcgtaa tggcaaatgc cagcgccccg gtaaccgttg
    10381 ccgcgccgcc catgtccgac ttcatcgagt ccataaacgc agtctgtttg atgctgtagc
    10441 cgccggagtc aaaagtgata cctttaccta ccaggcacgc gtacactggc gcttctttat
    10501 cgccagttgg gttgtaatcc agcgccagca ataccggaga acgttctgaa ccgcgtccga
    10561 cggtgtgcag ccccatataa ccttgctcac gcagatcttc gcctttggtg atccgataag
    10621 tcacacgatc gctcgcgacg ttgctgatca gatcaacagc acgctgtgcc agttgcgatg
    10681 gtcccaattc ttctgccggt gcgttgatgg tgtcacgcac ccagtcgatg atcatcaggc
    10741 ggttatccag ttcctggcgc tgggcatcgt ccagatccgg ccactccact ttacgcgtgc
    10801 ctttcggggc tttgtaacct tgccagaatg cccagcatcg atccgcatcc cagccttcac
    10861 cgcttaactg aacatgcttg atgcccagac cgtcaatctt gcgcgccgca cgctggatca
    10921 gccccagatc gtctgccccg ttcaaatgca gggtaatgcc gtcattatta atgctgtaag
    10981 ttgctttttc tccccagcgc gcgtcggcag gttgggtaga gagggtaatc ttcatcgctt
    11041 ctgtcatttt agttatcctt ctttgtaaaa gggccgccag ttggcagccc caggattttt
    11101 ccgctaaatg attcgcgttg caggaaggcg gcaagtgagt gaagccccag gagcatagat
    11161 aactatgtga ctggggtgaa cgagcgcagc caacgcatct gcggcgtgaa gcatgacgcg
    11221 gaaattattc ggcctcgtcc agccagacta acaaaatcgc ttcgaggatt ttctcgttgg
    11281 atgcctgcgg gtcgtcgtcg aaatcttcca gatcgcaaat ccactgatgc atatcggtga
    11341 atcgaaccgt tttcggatca agatcgggat acgcatcgta cagtgcttcg ccaatttcgc
    11401 ggctatcggt ccacttaagt cccatactaa cctctgttaa tgctcacgcg catggttgat
    11461 cgtgtaacgc gggatttcga ccactaaatc ttcgtcggtg acgcgcgcct ggcagcttaa
    11521 acggctttcc ggctccagtc cccaggcttt gtccagcatg tcgtcttcct gctctgagct
    11581 ttctggaagc gagtcaaaac cttcacgaac gatgcagtgg caggtggtgc aagcacagga
    11641 tttttcacag gcgtgttcaa tctcgatacc gttacgcaga gctgcgtcga gaatggtttc
    11701 accgctatta gcttccagaa cagcgccatc agggcagaga tcctgatgag gcaaaataac
    11761 aatctttggc atattaaacc tcgtccacgg aatggccttt cagcgcacga cgaaccgact
    11821 ggtccatgcg gcgagcggcg aaatcctggg tttgtttgtc tacgttttta atcgcttgtt
    11881 cgatggcgtc aacgtcatcg ccctgcgcca cttcactcag gtgagcggca gcatcgtcaa
    11941 tgacctgacg ttctgcggcg cttaacagcg cggcatcagc agccagcgcg ccgtgcagac
    12001 tttccagcac acgcgccgct tctacttttt gttctgccag cattcgggct tttacgtcct
    12061 gctcggcata gctcattgag tctttgatca tcgaagcgat ttcgctatcg gtcagaccgt
    12121 aagacggttt gacctgaata gacgcctcaa cgccggtgga tttctccatc gccgtcacgc
    12181 tcaaaagacc gtcggcatcg acctggaacg tcacgcgaat atgcgcaccg ccagccggta
    12241 gcgccggaat accacgcagc gcaaaacgcg ccagtgagcg gcagtcctgc accagttcgc
    12301 gctcaccctg cattacatgg atagacatcg ccgtctgacc atctttaaag gtggtgaaat
    12361 cctgagcgcg ggccactgga atagtggtat tacgcggaat cactttctcc accaggccgc
    12421 ccatcgtttc gaggcccagc gacagtggga tcacatcaag caacagcatt tcgctgtctg
    12481 gcttgttacc caccagaata tccgcctgaa tcgccgcgcc aatagcgacg actttatccg
    12541 ggtcgatgga agtcagcggt ggacgaccga aaaattcgcc tacccgttca cgcaccagcg
    12601 gcacgcgagt agaaccgccc accatcacca cttccagcac ttcatcagct tctacacccg
    12661 cgtctttcag cgcgcgacga caagccaata aggttcgttt taccagtggc gcgatcagtt
    12721 cattgaattg ttcacggctg atttcgccct gccagcccgc aacgttaacg gtcacggagt
    12781 ccgcatcgct cagcgcgatt ttggctgcaa tggcggcatc cagcagttca cgctgaacgc
    12841 ggttatcgct acgatcagga atgcccgcct gctcgcgaat gtaatccgcc agcagatggt
    12901 cgaaatcatc gccgccgagc gcggaatcac cgccggttgc cagcacttca aacacgccgc
    12961 gacttaagcg cagaatggaa atatcaaacg tcccgccacc gaggtcataa acggcgatca
    13021 cgccttcctg accggaatcc agcccgtagg cgatagccgc agcggtcggt tcgttaagta
    13081 agcgcaggac gtgaaggccc gccagacgcg ccgcgtcttt ggtgccctga cgctgggcat
    13141 cgtcaaagta cgccggaacg gtgataacta caccatccag ctcgcctgcc agggcttcag
    13201 ttgcccgcgc cgccagtgct ttgaggatgt ccgcagaaac gcgcaccggg ttcagcagcc
    13261 ccgccgccgt ttcaatcatc ggcaggccgt tttcgctggc ctggaattga taaggcagat
    13321 gcggatagcg ttgctggata tcagccagcg agcgtcccat caggcgttta acagaactaa
    13381 ttgtgttggc ggtatcgagc gctgcattag tacgcgcgtc ataacccacc gaatgccctt
    13441 gctgttgata gtgaacaaca gatggcagca ggtgacggcc ttcatggtcg gctaacgttt
    13501 cggcctgacc gctgcgcact gtcgccacca gcgagttggt tgtgcccagg tcaataccgg
    13561 ccgccagacg acgctgatgc ggcgcagcac tcaaaccagg ttcactaatt tgtaataagg
    13621 ccatgtttag cttccagaaa ttaaaaatcg agcagttttt cttcgagttg ttcggcactg
    13681 cttcgcagtt tatcgagaaa acgcagctta cgcacggtat ccgccgccgc gtcccaggtc
    13741 tcgttgtcta actgttcaac catcaactga tggcgggtat caaacatctt tttcacacgt
    13801 ttgataaagc tttccagccg cgcttcatct ttcgcctgtt cgatctcgtc cagctcttcg
    13861 cgcagctcca actgttccat caggaacgcg gtgtcgcgca cagtatgctg ctcgctggcg
    13921 agatcaaagc cgtgcaaaga aagcaaatat tccgcgcgca ttaacggatg acgcagcgtt
    13981 tgccaggcct gattaatggt tgcagattgc tgtacggcgg cgagttgttc cgcctggctt
    14041 ccgctggcga atttatcagg atgatactga cgttgtagat cctgaaaacg caggctcagc
    14101 gcctgggtat cgagttgata acgggcaggc aagccaaaga gggtgaagta atccataaca
    14161 ttctcagggc tgcgatccgc cgcgataacg gcgaatcagg taaaacaacc cccacgcgca
    14221 ggcgaccacg gtggggttat cggtatgcgc atcaaacgtg gaagctttcg ccgcaaccac
    14281 actcatcttt aacgttcggg ttggtgaatt taaacccttc gttcaggcct tcttttacga
    14341 agtccagctg cgtaccgtcc agaaattgca ggcttttgcc atcgaccacg actttcacgc
    14401 ctttgtcttc aaacacgatg tcttccggcg tcggttcgtc aacaaattcc agtacataag
    14461 ccatacctga acatccggag gttctcacgc ccagacgtag gccaaaccct ttgccacggt
    14521 tagccagaaa ggtatttact cgcgctgctg cactgtcgct aagtgtaatc gacataacca
    14581 aacctcaact cttattttgc ttcacgtttg cttttatagt ccgcaatggc ggctttgatc
    14641 gcgtcttctg ccagaataga acagtgaatt ttcaccggcg gcagttcaag ttcttcagca
    14701 atatcggtgt ttttgatagc ctgcgcttcg tcgagagact tccctttcac ccattcggtg
    14761 accagggagc tggaagcgat agcagaaccg cagccgtaag ttttaaaacg cgcgtcttca
    14821 atgatacctt catcgttgac tttaatctgc aacttcatta cgtcgccaca ggccggtgca
    14881 cccaccatgc cgctgccgac gttctcgtcg ttgttgtcaa aggaacccac gttacgcgga
    14941 ttctcgtaat ggtcgataac tttttcgctg taagccatta taaattctcc tgattccgat
    15001 accgattaat gatgagccca ttcgatgctg ttcagatcca cgccctgctt gtacatttcc
    15061 cacagcggag aaaggtcacg cagacgaccg atggatttac gaactaactc gatggtgtag
    15121 tcgatctctt cttcagtagt aaaacgacct aaagagaaac ggatagagct atgtgccagc
    15181 tcgtcgttca gccccagcgc gcgcagcacg taggacggtt cgaggcttgc tgacgtacag
    15241 gcggaaccgg aagaaaccgc gagatctttc agcgccataa tcagcgactc accttcaacg
    15301 tagttgaagc tgacgttgag aatgttcggc gcaccgtgtt ccaggtcacc gttcaggtaa
    15361 acttcttcga tatctttgat gccgttccac agacggttac gcaggccgcg cagacgttcc
    15421 atctcggtcg ccatctcttc ttttgcgatg cgataggctt cgcccattcc gacgatctgg
    15481 tgaacaggca gagtgccgga acgcataccg cgctcgtgac cgccgccgtg catttgcgct
    15541 tcgatgcgta cgcgcggttt acgacgcaca tacagcgcac cgataccttt cgggccatag
    15601 attttgtgac cggagaaaga catcaggtca actttcaact ggctcaggtc gataggcagt
    15661 ttgcccacgc tctgggttgc atcaacgtga tagataatgc cacgagcacg gcacatttcg
    15721 ccgatagccg cgatatcctg caccacgccg atttcgttat ttacgtgcat gatagaaaca
    15781 aggatggtgt cgtcacgcat cgctgcttcg agttctttca ggtcgataat accgttacgc
    15841 tgcggtgcga ggtaggtgac ttcgaaacct tcgcgctcca gctgacggca ggtatccaat
    15901 accgctttgt gttcggtttt gctggtgatt atgtgcttgc cttttttctg ataaaagttg
    15961 gctgcacctt tgatcgccag gttgtcagac tcggttgcac cagaggtaaa gacgatttca
    16021 cgcggatcag cgccgaccag atcggcaatc tgattacggg cgatatctac cgcttcttca
    16081 gcctgccagc cgaaacggtg agaacgggag gccgggttac caaaggttcc gtccatcgtc
    16141 ataaactgca tcattttctc ggcaacacgc gggtccaccg gcgtggttgc ggagtagtcg
    16201 agataaatcg gtaatttcat tgctctataa actccgtaca tcactcaatg caaggaacca
    16261 ggctaccggc tggatgtacg accgtgttta cggagcgcga tacgccccgg cctgattctg
    16321 aatacttttt attaagcgcg taacttaacg tcgatcgcgt cttgtgtgcg ggtgcgtggc
    16381 gcgtcgtgag tatgctgacg accagacaca tccagcactt cctggttgtt aaccagttcg
    16441 cctaaagtaa tgttattgag aaaaccggta agacggtcgc tcaaatcacg ccacagcgcg
    16501 tgggtcaggc atttatcgcc gccctggcag ccgcctttac cctgacaacg ggtggcatct
    16561 acagattcgt caacggcgct aatcacttcg ccaacggcga tgctgctggc atctttgcct
    16621 aacagataac caccgcctgg tccacgtacg ctggaaacca gaccattttt acgcagacga
    16681 gaaaacagtt gttccagata agaaagggaa attccctgac gttcggaaat atcagccaac
    16741 ggtaccgggc ccgcttcaga gttgagcgca acgtcaagca ttgcggtcac ggcatagcgc
    16801 cctttagatg tcagtctcat gtcttacttc acctcaaact cgcccctgcc cggggttttt
    16861 tattgtaaaa gtgggggtat tgcatagcag ggtcaagtct gacattcccg agtaaattgg
    16921 tcaactattt acttgactga tttagtcggg tatttaacct tcagtgccat ttttttatcg
    16981 tggcgtttgt acctgttatc cttccagacc atgggcgggt tgagggcaat tattcgcatt
    17041 agcgcaggcc tgttcccctc accctaaccc tctccccgaa ggggcgaggg gaccgtccgt
    17101 gcacttatct ggcttagccc tcaggcctta ttacctttat tctgctgctc tatagaagcc
    17161 agaatcccgc gcaggatatt caattcctgg ctttccgggc gcgcacgggt aaacagacgg
    17221 cgcaatttat tcatcacctg ccccggatgg ttttcacgga taaaaccggt tgccagcagc
    17281 gtttgttcca gatgaccata aaaacgctcc agatcatcga ccagcggata cggcgtctct
    17341 tcatgctcga cctgctcgcc attttcctga gttgccagcc aggccatacg cacttcatag
    17401 gcaataacct gaaccgccat cgccaggttc agcgagctgt attcagggtt agcggcaatc
    17461 gcgacatgat aatgacactt ctgcaactct tcattggtca ggccgacacg ttcgcgacca
    17521 aacaccagcg ccaccggggt atttgccgct tcagcaacgc ttttcagacc gcattcgcgc
    17581 gggtcgagca tcggccacgg cagcgtgcgg gaacgtgcgc tggtgcccac caccaggcta
    17641 caaccggcta acgcttcgtc gagcgtatcg acgatgtgag cattaccaat cacatcgctg
    17701 gcccccgccg ccagggcaat cgcctgggag tcaggtttca ccagtggatt aaccagccac
    17761 agattggtta atcccattgt tttcatggca cgggcaacag aacccatatt gccggtgtgt
    17821 gacgtctcca ccagcacaat tcgaatattt tgcagcattg tctttcttcg tctaaagatt
    17881 attcgcgcat cttatcataa aacgaagaca gatgccgatc tcgctgctat actctgcgcc
    17941 gttttcccgt tctttaacat ctagtgagag agaccgatgc atccgatgct gaacatcgcc
    18001 gtgcgcgcag cgcgcaaggc gggtaatttg attgccaaaa actatgaaac cccggacgct
    18061 gtagaagcga gccagaaagg cagtaacgac ttcgtgacca acgtagataa agctgccgaa
    18121 gcggtgatta tcgacacgat tcgtaaatct tacccacagc acaccatcat caccgaagaa
    18181 agcggtgaac ttgaaggtac tgatcaggat gttcaatggg ttatcgatcc actggatggc
    18241 actaccaact ttatcaaacg tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa
    18301 ggccgcaccg aagttgctgt ggtatacgat cctatgcgta acgaactgtt caccgccact
    18361 cgcggtcagg gcgcacagct gaacggctac cgtctgcgcg gcagcaccgc tcgcgatctc
    18421 gacggtacta ttctggcgac cggcttcccg ttcaaagcaa aacagtacgc cactacctac
    18481 atcaacatcg tcggcaaact gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg
    18541 gcgctggatc tggcttacgt cgctgcgggt cgtgttgacg gtttctttga aatcggtctg
    18601 cgcccgtggg acttcgctgc aggcgagctg cttgttcgtg aagcgggcgg catcgtcagc
    18661 gacttcaccg gtggtcataa ctatatgctg accggtaaca tcgttgctgg taacccgcgt
    18721 gttgttaaag ccatgctggc gaacatgcgt gacgagttaa gcgacgctct gaagcgttaa
    18781 tgactcaggc gggtgatatc actcacccgc cctcgccttt caggcgctat tccgaaatac
    18841 ttcctcaccg ctttactttc tttcgtcact ctcccaccat ctttccccga ttaatggatg
    18901 ataaagaagt cgcaaggaaa ggttatggca ctgccagtga acaaacgcgt tcccaaaatt
    18961 ctgtttattc tctttgttgt tgccttctgc gtttatttag tgccgcgcgt tgccatcaac
    19021 ttcttctatt atcctgacga taaaatttac ggtcccgacc cctggtcggc ggaatccgtc
    19081 gaatttacgg ctaaggacgg tactcgtctg caaggctggt ttattccttc ttcgacgggc
    19141 cctgctgaca acgccatcgc aaccatcatt catgctcacg gcaatgccgg aaatatgtcc
    19201 gcccactggc cgctggtcag ttggttaccc gagcgtaatt tcaacgtttt tatgtttgat
    19261 tatcgcgggt ttggtaaatc aaaaggcacg ccgtcccagg ccggattgct ggacgatacg
    19321 caaagcgcca tcaatgtggt gcgccatcgc agtgatgtaa acccacaacg gctggtgctg
    19381 ttcgggcaga gtattggcgg ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa
    19441 ggcatacgtg cagtgatcct cgactccaca tttgcctctt atgcaaccat cgccaaccag
    19501 atgatccccg gcagtggcta cttacttgat gagagttaca gcggcgaaaa ttatatcgcc
    19561 agcgtcagcc cgatcccgct tttactcatt cacggtaaag ctgatcacgt tatcccatgg
    19621 cagcacagcg aaaagttgta tagcctggca aaagagccaa aacggttgat cctaatcccg
    19681 gatggcgaac acattgatgc tttttccgat cgtcacggcg acgtttatcg cgaacagatg
    19741 gtgaacttta tccttagcgc gttgaatccg cagaactaac ccatgatcgc tagcacgata
    19801 atcattcaca aaatcacctt aagacatgct aatccactgg tcaggacagt ttaagatgag
    19861 aaaaattctg tgacgcttgc caacatttct gatgattagc attcccttcg ccatttccct
    19921 gagcaaactt tagctattct tatcaattat gcttatggga gatctacaga tgatgcctac
    19981 gcttgctcca ccatctgtcc tttcggctcc ccagcgccgc tgccagatct tgctgacgct
    20041 ctttcagccg gggttgaccg ccaccatggc aaccttcagc gagcttaatg gtgtggatga
    20101 tgatattgcc agtcttgata tcagcgaaac aggacgggag atcctgcgct atcatcaact
    20161 cacactgacg actggttatg acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg
    20221 tttgtgttta tttcactggc tacgacgtgg tttccgtctg tgtccgtcat ttattaccag
    20281 ccaattcacc cccgccctga agagtgaact gaagcggcgc ggaattgcgc gtaactttta
    20341 cgacgatacc aatctacaag cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt
    20401 tgaatcgcgc gatattcatt tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca
    20461 cgctggaatt acgcctcagt ttaatccgct ccaacgtcgc tgggcggaat cctgccttga
    20521 atttcaggta gcgcaggaaa ttggacgcca ctggcagcgt cgggcgctcc agcctgtacc
    20581 acctgatgag ccactgttta tggcactact tttttccatg ttgcgggttc ccgatccatt
    20641 gcgggatgcg catcagcggg acagacaatt gcgtcagtct atcaaacgtc tggtaaacca
    20701 ttttcgtgag ctgggaaatg ttcgttttta tgatgaacag gggttatgtg atcagcttta
    20761 tacccacctc gcccaggcgt taaatcgcag tttgtttgcc atcggtattg ataataccct
    20821 gccggaagag ttcgccagac tgtatccacg cctggtacgc accacccgcg cggcgctggc
    20881 cggatttgaa agtgaatacg gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt
    20941 gattttcggt gcctggctaa tgcaggaaaa cgatctgcat gaaaaacaga ttattctact
    21001 gaccgggaat gatagcgagc gagaagcgca gattgagcaa cagttacgcg aactaacgtt
    21061 actgccgctc aacattaaac atatgtcggt aaaggcattt ttgcagacag gcgctccgcg
    21121 tggcgcggca ctgattattg ctccttatac catgccgtta ccgctctttt caccaccgct
    21181 gatctatacg gacctgacac tgacaacaca tcaacaggag cagatccgca aaatgctcga
    21241 atcagcatga gggaacaact ttcgggcgca aaaacattgc cggaagcgcc accagcgcca
    21301 tcacccagaa cacgccgtgg cccagatatt gatacaggaa accggcgaaa acggtcatga
    21361 tagcgatact gccgcccatc gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc
    21421 taccctggcg agcagcaata tagcgcatgg cggccaggtg gcagaccgtg aaggtgccgc
    21481 aatgcagaat ttgcaccact atcaaccacg gcaacgccgt agttgctccc ataatgcccc
    21541 agcgcactac gccgcaaatc gctgagatca acagcatatc gcgtgcacta caacggcgga
    21601 aaagtttatt actcagtgca aagataatga cttccgccac cacgcccagc gaccacaaat
    21661 accccaccgc cgaggccgag tagccagctg cctgccagta aatggcgcta aaaccgtaat
    21721 aggccgcatg tgccccctgc aataaacaaa cgcaggccag aaagcgccag ttctggcgaa
    21781 ccagcgccaa ccacgctgac caaccggtgc tctcctgctg gcggcttgcc ccttgtggct
    21841 gaatcgtcgg acggatgaga aagccgagca gcatggatgc cacgcccaac gtcaacagca
    21901 cgaggatcac ccgataatca aacatagtga ccagtttgcc cgtcagcgcc gagccaatga
    21961 caaacgccac cgagccccac agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc
    22021 acgtattcgc cagtgcatcg gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa
    22081 tcatcaccag catcagccac gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa
    22141 gtgtcagcag tgccagcacg cgcaaggcgg aaatcaggcg agaaggatcg ctgacgcggg
    22201 gcgcgatgag caaactcccg agaaaacggg caaccagacc tgcccccaat aacaggccga
    22261 tggtttctgg cgttaaacca atccctttaa gccagacgct ccagaaaggt agaaaaatgc
    22321 cgtaactaaa aaagtatgtg aaatagccga gcgccaacca gcgcgtggat tgcaaaacca
    22381 tgagtccctc ccgtcaggag gcgttagtct ggagttaatc taatgttggt gcaagttgaa
    22441 aaataagaat attcgagcct gcttcccgat gtctgtaacg ccagttcaca agcactcaaa
    22501 aactgcgatc cgtgacgcat gttgcaaaga taatgcgtaa ttatgccgtt acgcttgcca
    22561 aacgttcctg cacaatggcg atgaaatcgc gtaatgccgg cttcatctcc cctttcttcc
    22621 acgccatcag taaagcaatg gaaggcacat ttccggcaat gggacgaaaa acaacctgcc
    22681 cggtattaaa attattcata taaccgggta tcaaagtgac gcccagcccc atgcccacca
    22741 gattcatggt caccagaata ttcgttgcca cctggacgat atttggctgg ctgttttctt
    22801 gtgcaaacca cgctttaacg atcggcgcaa gcgaaccgga atacgccgga tcggtactga
    22861 cgaaattcac gccatccagt tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat
    22921 caaccggtaa caccaccact aatggttcgt caaaaagctc cagataatca atctccgggc
    22981 tataaacagg atggcgcatt aagccgacgt cgagttcacc acggcgaatt ttttcctcct
    23041 gttgcgtggt gattaaactc accagctcaa tcaaggtgtc tggctgtctg agacgaaaca
    23101 tcggtaatac ttttggcagt aaattcactt ccgccgatgg cacaaagcca atggttaatt
    23161 gtctgtcttc ctgaacaatt ttccgcgccc gtaatttggc attttccgct tgttcgagga
    23221 ttgccagcgc gtcctggaga aaacattctc ccgccgccgt caacgcgact ttgcgcttat
    23281 ccctcaccag taacggaaca ccgacacagt tttcaagatc gcggatctgg ctgcttaacg
    23341 aaggctgtga ggtatgcagt ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg
    23401 cgacgaaata gcgtaaatgc cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc
    23461 tcagctggta gaaattaact atttcacata ttagcaacca accagcaaca tccttatgac
    23521 acaaaaatag aaggtcaata catcttatct ttcaggatta aaaaatatga ccacaccctc
    23581 agatttgaac atttaccaac tgattgacac ccaaaacggt cgggtcactc cgcgtattta
    23641 taccgacccg gacatttacc aactggagct tgagcgtatt ttcggtcgtt gctggctatt
    23701 tctcgcccac gaaagccaga tcccaaaacc cggtgatttc tttaacacct acatgggaga
    23761 agatgcagtt gtcgtggtgc gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg
    23821 ccgccaccgg gccatgcgtg tgagttatgc agattgcggc aacactcgcg cctttacctg
    23881 cccgtatcac ggctggtctt atggcattaa cggcgagttg atcgatgtac cgctggaacc
    23941 tcgcgcctac ccacaagggt tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt
    24001 ggagagttat aaagggctga tttttggcaa ctgggatacc agcgcaccgg gcctgcgtga
    24061 ttacctgggt gacattgcct ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac
    24121 cgaaattgtc ggcggcgtac aaaagtgggt gatcaactgc aactggaaat tcccggcaga
    24181 gcagttcgcc agtgaccagt atcatgctct gttcagccat gcttctgccg ttcaggtatt
    24241 aggggcgaaa gatgatggca gcgataagcg cctcggtgat ggacaaaccg cccgcccggt
    24301 gtgggaaacc gccaaagatg ccctgcaatt tggtcaggat ggtcacggca gcggcttctt
    24361 ctttactgaa aaaccagatg ctaatgtctg ggtcgatggc gcagtttcca gctattaccg
    24421 cgaaacctat gccgaagctg aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg
    24481 tcataacaat attttcccga cactttcctg gctcaacggc actgccacac tccgcgtctg
    24541 gcatccgcgc ggccctgatc aagttgaagt gtgggcgttc tgtattactg acaaagccgc
    24601 ctccgatgaa gttaaagccg cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg
    24661 ttttctcgag caggatgact cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca
    24721 ccgcgcccgc aacagcaaac tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga
    24781 cgacggcatt cctggcatta ctaactatat cttttcagaa actgctgctc gtggaatgta
    24841 ccaacgctgg gccgatcttc tgagtagcga aagctggcag gaagtgctcg ataaaaccgc
    24901 cgcttaccag caggaggtga tgaaatgagt gcgcaagttt cactagagtt acatcaccgc
    24961 attagccagt ttctctttca cgaagccagc ttactggacg actggaaatt tcgtgactgg
    25021 ctggcacagc tcgacgaaga gatttgttac accatgcgca ccacggtgaa cgcgcaaacg
    25081 cgcgaccgcc gcaaaggtgt tcagccgccg acaacctgga tttttaatga caccaaagac
    25141 cagctggagc ggcgaatcgc ccgtctggaa acgggcatgg catgggcaga agagccgccg
    25201 tcacgcaccc gccacttaat cagcaactgc cagataagcg aaaccgacat cccaaacgta
    25261 tttgctgttc gggtgaatta tctgctttat cgggcccaaa aagagcgcga tgaaacgttc
    25321 tatgtcggga ctcgttttga caaagttcgc cgtctggaag atgacaactg gcgcttgctg
    25381 gaacgggata tcgtcctgga tcaagcggta atcacttccc ataacctgag tgtactgttc
    25441 tgatgaatcg aatttatgcg tgtcccgttg cggatgtgcc ggagggtgag gctctccgga
    25501 tcgatacctc gcccgtcatc gccttgttca acgttggcgg cgagttttat gccattaacg
    25561 atcgttgcag ccatggtaat gcgtcgatgt cagaagggta tctggaagat gacgccacgg
    25621 tggagtgccc gctacacgcc gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc
    25681 ccgccaccga tccgctcacc acttatccag tacacgttga aggtggtgac attttcatcg
    25741 acttaccgga ggcgcagcca tgagcgatct gcataacgag tccattttta ttaccggcgg
    25801 cggatcggga ttagggctgg cgctggtcga gcgatttatc gaagaaggcg cgcaggttgc
    25861 cacgctggaa ctgtcggcgg cgaaagtcgc cagtctgcgt cagcgatttg gtgaacatat
    25921 tctggcggtg gaaggcaacg tgacctgtta tgccgattat caacgcgcgg tcgatcagat
    25981 cctgactcgt tccggcaagc tggattgttt tatcggcaat gcgggcatct gggatcacaa
    26041 tgcctcactg gttaatactc ccgcagagac gctcgaaacc ggcttccacg agctgtttaa
    26101 cgtcaacgta ctcggttacc tgctgggtgc aaaagcctgc gctccggcgt taatcgccgg
    26161 tgaaggcagc atgattttca cactatcaaa tgccgcctgg tatcccggcg gcggtggccc
    26221 gctgtacacc gccagtaaac atgccgcaac cgggcttatt cgccaactgg cttatgaact
    26281 ggctccgaaa gtacgggtga atggcgtcgg cccttgtggt atggccagcg acctgcgcgg
    26341 cccacaggcg ctcgggcaaa gtgaaacctc aataatgcag tctctgacgc cggagaaaat
    26401 tgccgccatt ttaccgctgc aatttttccc gcaaccggct gattttacgg gtccgtatgt
    26461 gatgttggca tcgcggcgca ataatcgcgc attaagcggt gtgatgatca acgctgatgc
    26521 gggtttagca attcgcggca ttcgccacgt agcggctggg ttggatcttt aaggaagtac
    26581 gatgaaagaa aaaacgatca ttattgtcgg gggcgggcaa gcggcggcaa tggctgcggc
    26641 ctcgctacgc cagcaagggt tcaccggtga gctgcatctg ttttccgatg agcaacatct
    26701 tccttatgaa cgccctccgc tctcgaaatc catgttgctg gaagattccc cacagttgca
    26761 gtctgtgtta cccgctcact ggtggcagga aaacaatgtt catctgcatt ccggtgtaac
    26821 catcaaaaca ttgggccgcg acacacgaga gttagtgtta gccaacggcg aaagctggca
    26881 ctgggatcag ctttttatag caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc
    26941 actgggagaa cgctgcttta ccctgcgcca tgccggtgat gccgccagac tgcgagaagt
    27001 tctgcagccc gaacggtcag tcgtgattgt cggtgccgga actattggtc tggaactggc
    27061 tgccagcgcc acgcagcgca gatgtaaggt gacagtgatt gaactggcgg caaccgtcat
    27121 gggccgtaat gcaccaccgc ccgtgcaacg ctatctttta cagcgccatc agcaggctgg
    27181 tgtgcgcatt ctgctcaata atgccattga acatgtggtc gatggtgaaa aagtagaact
    27241 gacgctgcaa agtggcgaga cgcttcaggc cgatgtggtg atttacggta ttggtatcag
    27301 cgccaacgac caactggctc gcgaggccaa ccttgatact accaatggca ttgtcattga
    27361 tgaggcttgc cgcacctgcg atcccgcgat ctttgccggt ggcgatgtgg caatcactcg
    27421 tcttgataat ggtgcactac accgctgcga aagctgggaa aacgccaata accatgcgca
    27481 aattgccgct gccgcaatgt tagggctacc gcttccgcta ctgccgccgc cgtggttctg
    27541 gagtgatcag tacagtgata acttacagtt tattggcgat atgcgtggcg atgactggct
    27601 ttgtcgtggc aacccggaaa cccagaaagc gatttggttt aatctgcaaa acggcgtgct
    27661 tatcggtgcg gtaacgctga atcagggacg tgagattcgc tcaatccgca aatggatcca
    27721 gagcggcaaa acgtttgatg cgaaacagct gacagatgag aacatcgcgc ttaaatcact
    27781 gtaaccagga taattagcga atatctcaac gcctggggcg tggcgaggta ccagagtgtg
    27841 tattacgttt aaatcacatt atcttgcaaa gggattggtt atgaactcac tacgttattt
    27901 tgattttggt gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg tggtcctaat
    27961 tttcattatt tttggttttc ccaaaatgat gggctttgac ggtacggtcc aatatatggc
    28021 ctcgctgggc gcaccgatgc cgatgctggc agcgattatt gcggtagtta tggaagtgcc
    28081 cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg ctggcggtac tgtttatttt
    28141 ctacacgctg ggtacggcgg tgattggtca ccattactgg gatatgaccg gcgatgccgt
    28201 cgggccaaat atgattaatt tctggaagaa tgtcagtatc gctggcgcgt tcttgctatt
    28261 ggcaattacc gggccggggg caatttctct cgatcgccgt taggaaagat gccggatgcg
    28321 gcgtgaacgc cttatccggc attttataaa ttacagccac tctacccgca acgacatctc
    28381 tgaggttgtt gattcccccg gcgcaagcgc aatgaggtca cccccttccg ggcgatgatg
    28441 gtcatccggc gcatggctca tcggctccag acagaagaaa tcaaacgcat atcctttatc
    28501 aaacgccggg tcggaaacaa agatgaaata acacggtgca ggtggcgtcg tttctatgat
    28561 gatggcataa ccctcctgcg gctgctcgat gcgggcctga ccattccatc cggcaaaacc
    28621 attgttcacc cactggcgcg gcaacggcgc tagctggtta aaatccagtt cttgcggtag
    28681 ctgctcgcaa aactcacccg ccagccactg ctcccgctcc agccagtaac cgctcgcctg
    28741 tgcctgaatc cgcgtttgcg gcgacaacgg aaaataagga tgccagccgg taccaaatgg
    28801 cagcgtctct gccccttgat tggtgacaga gagcgtcacc gtcagcgtat ccgccgttaa
    28861 atgaaacgcc tgacttaccc gatagtgata gacaccgctg cgatgttcat acaccagaca
    28921 caaactatca tcgctgtgcg agacacattg ccactcgccc agccagccat cgccgtgtag
    28981 atagtgcgca tcccactcaa cattcggttg cagttgatac tcacgcccct gccagacaaa
    29041 acggttgccg ctcacccggt ttgcaaatgg caccagcgga aaacaagagg catcggttgc
    29101 cacaccgctt tttttaccag gacgtaagag cggcgtcgta tcgcgccaga agccttcgat
    29161 tacgccgccc tggtcagaaa cgcccagctt tagcgacccg tgggataagg tatagatggt
    29221 catccgctaa ctccttaatc cgggaagtta atcacaactt tcccgcattt gccgctcgcc
    29281 atcagcgcat aggcatcacc tgcctgttcc agcgagaagc gatgggtaat ggcgttacgc
    29341 ggccacagct tccagtcagt caaatcgtgg gcgcattttt ccatatggaa cagactggtc
    29401 acccaggagc caataatccg ccgttgatgg tgcatcagat cggcgctgac ctcgaattcc
    29461 acttttccgg tttcaccaat gtaaaccacc cgcccccagt cagcggtgga ttgcagtgcc
    29521 agtaagcgac ctgcggcatt accggaacaa tcaagcgcaa catccgcgcc gccgtgagtg
    29581 agttcggcga ttatctgcgg cagaccttca gtggtcgcta aatagccgtg atccatcact
    29641 cctaactgtt ttgccatcgc cagacgttcc ggcagcatat caacgccgat aatccgtttt
    29701 gcaccgcgac ctttcgccag catcatcccc atcatgccga ctggccccag accgaccacc
    29761 agcacgttat cactgccgga aacttcgccg cgcaaaatcc cttcataggc cgtgccaacg
    29821 ccgcaactga taaacgcacc atcttcgtag ctcagcgcat ccggcaggag gatcagatct
    29881 ttttcttccg ccagcaagta ttcggcatga ccgccgtcac gctgccagcc gtaagccgct
    29941 tttccttcgc cagtacaaga aataggaaaa ccgcgacggc agttcgggca aaaaccacag
    30001 ccagaaatgt gatacaccag cacgcggtcg ccctctttaa aatggcggca gccttgcccc
    30061 atcgccacaa tctgcccgca cggttcatga ccgttgataa atccctagta caacggttta
    30121 tcggatgccg ccgccgtagc gcggtgctgg tggtagatat agtggacatc gcttccgcaa
    30181 attccggagg atttcatttt gatcagtacc tggttaatcc ccggcgtcgg cactgcaact
    30241 tcccgcagat cgacggtcga atttcctggt aaataagctg ccagcatcgt tttcataaat
    30301 ccctcattaa caatacgatt aattttcatc cctgccgcac ccgcgccagg ggcgttaagg
    30361 tttagcgttt cgctttactg ctacgctggg tcagcaagat attcgccagc accgccacca
    30421 cgatgatgac gccgcgcacc acctgctgga aaaaggagtt aatcccgagc agcaccagac
    30481 cgttaccgat tagcgtaatc accagtacgc caagcagcgt accgaacaac gagccgcgac
    30541 caccggaaag cgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac tccagaccgt
    30601 ttgcggcacc tgcgttaccg gaaccgaggc gcgccgccaa caaaatgccg gtcaccgccg
    30661 ctaataatcc cgaaagggta aagataagaa tgcgtacccg acgaacgtta ataccgcaca
    30721 actgcgccgc cgtggcatta ccgccaacgg caaaaaccga gcgcccgaag gcggttttgc
    30781 ggctaatgaa cacaaacagc gcaaacagca ccatcatgat cagcgcggat accggcacac
    30841 cgagaaattg tccgcccagc cagtccagca cctcgttttc gtcaatcggc actggcagag
    30901 cgttggtcat aaacaagccc attccgcgca gggcgctcca cagccccagc gtggccacga
    30961 aacttggcac gttaaacacg ccacgcagca ccccggcgag cgttcccatc agcgcgccta
    31021 acagcaacac cagcagacaa gcaatcgcca acggaacttc aaattgcagc aaaaatgcca
    31081 ggcataccga gacaaaagcc accatcggcc caacgctgac atcaatttca ccggagataa
    31141 taatcagcgt catcgcccag gcggcaatcc caatggtggc ggcatcgcgc agcacgttca
    31201 tctggttatt caatgagata aagccaggcg cgttcaggga gaagaccaga taaagaatgg
    31261 caatcaccac cagcaaaccg atctcattaa tatggtgact gacaaattgt ttgagcgaga
    31321 cgctcttgcc ctgcggcaat ggtaatgacg aagcagacat cttcaggttc ctcggtaaaa
    31381 ttcatcagtg cacagacaga atggcggaca tcagctcatc cacattgacc ggagcgtgaa
    31441 actcctgcga gaacgtgccg tgctgtaata acaggatgcg gtcacacacc aacggcaact
    31501 cctccacttc actggagata aacaccacgc tttttccttc ggcagccagc tcacggacaa
    31561 tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg tcgagcaaca
    31621 aaatctggct ggcggcatag acccaacgac cgatcactac tttttgctga ttgccgccag
    31681 aaagcgtgcc gatgggcgtt tcgctactgg cggccttgac cgtcatccgc tgcatcactt
    31741 cttcggtcag gcggcggatg gtggaccatt gcagcactcc gttggcgctg attttttgcc
    31801 gattggtcag cactgtattt tcgtcaacgc ccaaccaggg aatgatcccc gcttctttgc
    31861 ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg gggcgcgtga
    31921 ttttctcgcc gttgataaca atttcgccct gttcatacgt ctccagccca acaatcgcct
    31981 tcagcaattc actgcgccct gcccccagca gaccagcaat gccgagcact tcgccacgac
    32041 gtagcgtaaa gctgatatcc tccagcttgg gcttatggcg taacgcacgg acttccagca
    32101 cggcctgatc cacaatttcc tgaggtgcaa ccggcgcaat atcaacgtga tcgcgcccta
    32161 gcattagcga cacaatatga tgcgtggaag tgttttcgag catcacatcg ccagccacct
    32221 gcccgtcgcg cataacggtg gcactggagg caatgcggcg aatttcttcc atccggtggc
    32281 tgacataaat caccgccacg cccagtgctg acatcttttt caccgctctg atcaccagtt
    32341 caacttccgc actcgcaagc gaactggtag gttcatcaag aatgaccacg cgcggctcgc
    32401 ccttcatcac ccgcgcaatt tccaccagct gcttatgcgc cgggcttagt gttgaaacaa
    32461 gttgttcagg actaacgtca acgcccagcg cctgtaagca acgttgggca tcctgcgcca
    32521 tttgcaggta atcaatcatg ccgttgcggc ggggccactg accgaggcag aggttttccg
    32581 ccaccgtcag cccttccacc agacttaact cctgataaac cgcgcgtacc cccagttcag
    32641 cggcacggcg agtcagcgta gcttcgtcac cttccagtcg cgtctcgcca atccagattt
    32701 caccgctatc cggacgttca ctgccggtaa gcattcgaat gagggtcgat ttgcccgcgc
    32761 cgtttttgcc taacagcgca cgaacctcgc ctttattgag cgtgaagtta acgttatcca
    32821 acgcaacgac gccgggataa cgcttatttc ctgccaccac ttttgctacc gggactgcct
    32881 ctgttgccgt gaacataggg acctctgcga atcagcgatt agggcagacc atcaacgtgc
    32941 gttgccagcc actgtttacc gtcttccgtt ttggtataga gatcgatagg cacctgaatc
    33001 actttttcac cgtcggcttg tttattgata accttcaatg tttgcgcgaa aacagcattg
    33061 cccattttct taccggaaat atccactacc gctttcagca cctgattgtt ttccagctcc
    33121 tgagcaattt cggttgtcat atccgaaccg aaaacagcaa tttttccggc ctgattttga
    33181 ttacgtaccg cttttaccgc gccgagtgtc gcaccgcccg attcccccat aatggcgttg
    33241 agatccggcg tggagataat cagtttttca ccaacggaaa tcgctttatc taaaacagtc
    33301 ccttcctgat tagcgacaat ttgcgcgccg ggaacgcggg attttaatac ttcttcaaat
    33361 cctttacgtc gctgcacaca aacttcaaag gcttcgcaat tgatgacggc aattttcggc
    33421 tggtcaattt tattggcaat aaaataatcg gcggcagcgt tacccagttt tttaccaaat
    33481 tccagcgggt cacccaccag atacgccgag acatatttat cgaccccctt ttgattaata
    33541 caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacggcta
    33601 ctgccatttt cagacactgc cgataaaata atagcatcga cattacgcgc cacgagggta
    33661 tcaacaaagg tactttcttt cgaaatatca ccctgggcgt tagtttcaat taactgtacc
    33721 tgtactgaag aatcttttgc cgcatcctga actccctgac gcactccagc gtaatatccc
    33781 tgagtatcaa ggtatattgc gccaatggtc atttctttat ccgcagctct ggcaaatagt
    33841 gcgctaccta atagcgtagc cattaataat agattacggg ttgttctcat ttttttaggc
    33901 atagagcctc ctgtagggtt tttattaaca acggcttatt ctaattattt tgtgatgagc
    33961 ggcagcgcgt gccgctcaaa tattacagtt aatagggttt agtgaacgta gttaaaaata
    34021 aaaggcatca cggtggtgaa taatgccgct ttcggccagg ccggattaat cgccgtcaat
    34081 tcagcgcgag cagattcgta ttgcgcaatc tcgcctaatc ccgcggccgc cagcatcgcc
    34141 accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga caacaagtca
    34201 ggctgcgaga cggcaaagaa atccgcttcg atactggttt tcgccatctc ttgcgcccac
    34261 tgtttcattt cactaaacag ttgctgtgcg gtgtgttgtt cgcccagtaa tcgcagcgcc
    34321 attccttgcc agaagaggta atcaaccggc tgatcgttgt aatagctgtg gatgttaatg
    34381 gtgcgatcgc cggtcgccgc cagatgtaaa cagcgcgtcg cttcagtttc atcgccctgg
    34441 gctttggcgc atatcgcctg ccagaaccag atgtcgttat cagtttgccc cggtaaacgg
    34501 ccttcgctta aattctccgg ataatgcagc gcggcatgaa gcagttcgct ggcctgctgc
    34561 ggctctctgg catcaagatg ctgccaggcg cgtaataact ggttgaggat aaactgactg
    34621 gtgaccttcc cttccccgcc ttcccacggg tggaatttgc gcgtggcgag aatgtccgcc
    34681 gctttgtctg cctgccccgt gagatgccac aaattgagca gttctgcggt catgtcgtcg
    34741 cgtttcagcg caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtggtt
    34801 ccacttagct tatcaagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc
    34861 tgataagcat tatcaagata acgcgcggcc agctcataat cgtgttgctt attccacgca
    34921 tggatcgcta acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgttgc
    34981 cataaggcaa tggcttcgtt gtagctacgt ttgttgtagt agaagcaggc cagtaaatgg
    35041 cgagcaaacc agcactcttc aatactctcc agcgccgcca cttcttccag cgtattcggg
    35101 aaacggacaa actgcgggaa gacatcaatg gcttttgcga ccagttcgcc acgttcggct
    35161 ttcggcagca agctggcttg caggtaaagc ggcagcgtgc gctggcagtc cagtgcattc
    35221 agcatctctg ccgcgagggt gggcattccc cagttaatca gttgcccggc ggtcatcagg
    35281 gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagagctga ttcgctacga
    35341 ccatcgaacc agttcaggca ccacagagtg gcgttcagcg gataatcgcg cagcagtttc
    35401 tcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcagg
    35461 caaagcactt cctgattgat tgggcaggcg cgaagacttt gttggcaaaa atccagaccc
    35521 gcgtcgaagt taccattacg cgccgccagt cgtgccagac cataatagcc tccggcttta
    35581 ctgttgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga
    35641 cgttcgtaag cactggcgcg aatcaaactc gcctgtccgc actgcggatt tttgttcagc
    35701 gcatgtgcgc gtttcagagc ctgagtggca tacgccaccg cttgcgggaa atctgcgcgg
    35761 ttatactcca gcatcgccag cgccagatta cagcggtaat ccagcggatc cagcgccacg
    35821 ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga
    35881 ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgacagtgg cgctttggcg
    35941 atgtccggca acggcaactc ttgcggctga tgttcctgat aactcagtac aatattgccg
    36001 tcggcatcag agagctcaat ggtcagtctt tcaggattga taccgtgtaa cacgccctgg
    36061 atggcggtcg ctggtgtcag ggccacggca tcatcgagca acgcgtcgca tttgccgatt
    36121 tcgcggatcg ccaggcggta tccgttcaac ggagagatgg catacagccc ccactcaatc
    36181 ccccgctcac tacgctggag tttgattacc gcatcgcggg aggcgttttg caccatgccc
    36241 agagaatggt aaggcaggaa aaactgctca aaacgcttct cttcgtaagc atcaagccgg
    36301 gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta
    36361 ttatcagtta ggctcttatc ccacgcctgg ccaaattcac tgtgtcccca gctccactgc
    36421 tttttacctg gcgcaatatg gtggttggca acgtgtagca aaccgccatc ttcatcgtga
    36481 caccacgcgc caacaaaatc gtactgtgat ttttcagcca tatatgaggt tggaacgggc
    36541 acatttttat agcgagaaat gtccactcca gcggagtagt ccactttgta gtaagtgccg
    36601 gtggcgatag ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc cgttacatcc
    36661 ggcggaaaga cgctctgatg cccttcaccc cctttcactg ccgggttggc ccaccacaag
    36721 aaatgacgcg gcgtggcgtt gccgttatag acgcggctgg cgatttccag cgccgcccgg
    36781 tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc cgtttcgcct
    36841 acccacaccg tctgtgcgcc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag
    36901 gtggtcgggc gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggtccc
    36961 agcagcccca ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgctgtttc
    37021 actttatccc atgcgcgatg cacccgaccg cccaattccg gcaggatcat cactttgatg
    37081 tagtcgtttt ccagccacac cgcctgccag gatttcaggg ttttctgctc gctcagcgta
    37141 tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg
    37201 aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc
    37261 cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat
    37321 ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta
    37381 aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc caccataaca
    37441 aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc
    37501 ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa
    37561 aacgggtggt gaatatcgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc
    37621 tgattgcgcc ggaaggtgac tggacgctgt gtctgaacgt gacgcccacc agtattgagt
    37681 gtcaggttgc taatgcttgt ttaagtccga aaggtgaatt tgagtattta cagattgatg
    37741 caccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat
    37801 tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cggtcaggtt gatccagtga
    37861 ctggagtatc gcaaaccatg ccgcaagcgc cgtggacaac gccggttgag gtgaagtatc
    37921 tgctggaaga gaagctgggc attcgggtga tggtcgataa tgactgcgtg atgctggcgc
    37981 tggcggaaaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt
    38041 acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta
    38101 gcggacagat tggtcacacc atcgttaatc cggatggcgt cgtctgcgac tgtggacgtt
    38161 acggctgcct ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc
    38221 taaaatcaca accggttaat actcagcttg atcctgaaaa actgactaca gcgcagttaa
    38281 tcgctgcctg gcaaagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg
    38341 ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt
    38401 acggtcgcag ttgtgccttt ggtgagcact ggcttaatac tattattcgc cagacaggat
    38461 ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc
    38521 aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag acgcagttac
    38581 gacagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt tttcaacaga
    38641 ttaaccgttt cgcttatgcg taaaccgggt aacgtgcgca gatgtcgaga actttacctt
    38701 tgatgcgctc gataacggct tcatcattga tgctgtccag cacgtcacac atccagccag
    38761 ccagttcttt cgcttctact tctttgaagc cgcgacgcgt aattgccgga gtacccacgc
    38821 gaataccgga ggtcacaaac gggctcttcg gatcgttcgg tacgctgttt ttgttgacgg
    38881 tgatgttagc acggcccaga gcggcgtctg cttctttacc ggtcaggttt ttatcgacca
    38941 gatcaaccag gaacagatgg ttatcagtac cgccggaaac cactttgtag ccgcgttcca
    39001 ggaacacttc taccatcgct ttagcgtttt tagcgacctg ctgctggtaa gttttgaact
    39061 caggctccat cgcttctttc agagcaaccg ctttaccggc gattacgtgc atcaacggac
    39121 cgccctgacc accagggaaa acggcagagt tcagtttttt gtacagctct tcgctaccac
    39181 ctttcgccag gatcagaccg ccgcgcggac ccgccagggt tttgtgagtg gtggtagtaa
    39241 caacgtgagc atgaggaacc gggttcgggt agacgccagc agcaaccagg cccgcaacgt
    39301 gcgccatatc aacgaacagg taagcgccga tgctgtcagc gatttcacgc attttcgccc
    39361 agtccaccac gccggaatat gcagagaagc caccgataat cattttcggt ttgtgttctt
    39421 tggcttgttt ttccagatcg gcgtagtcga tatgaccggt agcatcaata ccgtaaggaa
    39481 caatgttgta cagtttaccg gagaagttaa ccggagaacc gtgagtcagg tgaccgccat
    39541 gcgccaggtt catacccaga acggtatcac ctggttccag cagcgcggtg tagaccgcaa
    39601 agttagcctg ggagccggag tgcggctgga cgttagcgta gtcagcgccg aacagttctt
    39661 tcgcacgatc gatcgccagt tgttcaacga tatcaacata ctcgcaacca ccgtagtagc
    39721 gtttgcccgg ataaccttca gcatatttgt tggtcagctg agaaccctgc gcctgcatta
    39781 cgcgcgggct ggtgtagttt tcggaggcga tcagttcgat gtgctcttcc tgacgtactt
    39841 tttcctgctc catagcctgc cacagttcgg catcataatc ggcaatgttc atttcacgct
    39901 ttaacatccg catctcctga ctcagctaac aataaaattt ttggccttta taggcggtcc
    39961 tgttggacaa cggcgaacag tataaccgaa tcattgtgcg ataacaggtc ttgacaaagg
    40021 aatttacgca aacgattacc ttcaggctac gcaaggcttt ggagaataaa gagcttgcaa
    40081 ccggaaacgg atttcttttc aggtttgtga tgcaaatttt tcacttcatc acattctttc
    40141 tgaaaaacac caaagaacca tttacattgc agggctattt tttataagat gcatttgaga
    40201 tacatcaatt aagatgcaaa aaaaggaaga ccatatgctt gacgctcaaa ccatcgctac
    40261 agtaaaagcc accatccctt tactggtgga aacggggcca aagttaaccg cccatttcta
    40321 cgaccgtatg tttactcata acccagaact caaagaaatt tttaacatga gtaaccagcg
    40381 taatggcgat caacgtgaag ccctgtttaa cgctattgcc gcctacgcca gtaatattga
    40441 aaacctgcct gcgctgctgc cagcggtaga aaaaatcgcg cagaagcaca ccagtttcca
    40501 gatcaaaccg gaacagtaca acatcgtcgg tgaacacctg ttggcaacgc tggacgaaat
    40561 gttcagcccg gggcaggaag tgctggacgc gtggggtaaa gcctatggtg tactggctaa
    40621 tgtatttatc aatcgcgagg cggaaatcta taacgaaaac gccagcaaag ccggtggctg
    40681 ggaaggtact cgcgatttcc gcattgtggc taaaacacct cgcagcgcgc ttatcaccag
    40741 cttcgaactg gagccggtcg acggtggcgc agtggcagaa taccgtccgg ggcaatatct
    40801 cggcgtctgg ctgaagccgg aaggtttccc gcatcaggaa attcgtcagt actctttgac
    40861 tcgcaaaccg gatggcaaag gctatcgtat tgcggtgaaa cgcgaagagg gtgggcaggt
    40921 atccaactgg ttgcacaatc acgccaatgt tggcgatgtc gtgaaactgg tcgctccggc
    40981 aggtgatttc tttatggctg tcgcagatga cacaccagtg acgttaatct ctgccggtgt
    41041 tggtcaaacg ccaatgctgg caatgctcga cacgctggca aaagcaggcc acacagcaca
    41101 agtgaactgg ttccatgcgg cagaaaatgg cgatgttcac gcctttgccg atgaagttaa
    41161 ggaactgggg cagtcactgc cgcgctttac cgcgcacacc tggtatcgtc agccgagcga
    41221 agccgatcgc gctaaaggtc agtttgatag cgaaggtctg atggatttga gcaaactgga
    41281 aggtgcgttc agcgatccga caatgcagtt ctatctctgc ggcccggttg gcttcatgca
    41341 gtttgccgcg aaacagttag tggatctggg cgtgaagcag gaaaacattc attacgaatg
    41401 ctttggcccg cataaggtgc tgtgatttga tgttgccgga tggaaacatc cggcaaccct
    41461 tgacgcggtt taaattgccg cgtcgtcctc ttcaccggta cggatgcgaa tgacccgtgc
    41521 cacgtcaaag acgaagattt taccgtcacc gattttgccg gtttgcgccg tgcgaataat
    41581 ggtatcgaca caggtatcga caatgtcgtc cggtacgaca atctcaattt tcactttcgg
    41641 cagaaaatcc accatatact ccgcgccgcg gtacagctcg gtatggcctt tctggcggcc
    41701 aaaacctttc acctcagtga ccgtcatgcc ggtaatgccg acttcagcca gtgcttcgcg
    41761 gacatcgtcc agcttgaagg gtttaataat cgcatcaatc tttttcatgc tattccttga
    41821 aaaggtcgcc tgtcttttga tctgctaaac gtaacacata acgccaattc attccttgaa
    41881 atcgtttgca tccagctcgt gtcgggaaag cagtttataa aattctgtcc ggttgcgccc
    41941 cgccattctc gccgcgtggg tgacgttgcc tttggtaatt tgcagcagct tacgcaaata
    42001 gttgagttca aactgattac gtgcctcaac aaaggttggc agcgccgtat tttcaccctc
    42061 cagcgcctgc tctactaatg catcgctaat caccggagat gaggtcagcg ccacgcactg
    42121 ttcaatcacg ttgaccaact ggcgcacatt acccggccag ctcgcggtca tcaggcgttt
    42181 catcgcatcg gtggaaaacg cgcggacaaa cggtttatgt cgctctgccg cctggcgcaa
    42241 caggtgattt gccagtagcg gaatgtcttc tgtgcgctcc gccagtgccg gaattttcag
    42301 gctgacaacg ttgaggcggt aatagagatc ttcgcggaat tccccgcgcg ccatcgcttt
    42361 tggcagatca cggtgagtgg cagaaataat ccgcacattg atatcaatat cgcggttact
    42421 gcccagcggg cgcactttac gctcctgcaa cacgcgcaac agtttgacct gcaacggcgc
    42481 aggcatatcg ccaatctcat cgagaaatag cgttccgcct tccgccgcct ggaataaacc
    42541 ttcgcgattg ctgacagcgc cagtaaacgc gccacgcgca tgaccaaaca gctccgactc
    42601 cagcaattgc tcaggcaatg caccacagtt aatagcaata aatggtttgc tattgcgcgg
    42661 gctggcgttg tggatagcct gggcgaaaat ctctttcccg gtgccgctct ggccattaat
    42721 caaaacgctg acgtctgatt gcgccaccag ccgcgcctgt tccagcaaac gcagcatcag
    42781 cgggctgcgg gtgacaatcg cctcgcgcca gcgttcgtcg gtggctggcg cggattgttc
    42841 cagcgcatca tcaattgcct gatatagcgc gtctttatca acaggcttgg tgaggaaact
    42901 aaaaacgccc tgctgtgttg cagcaacggc atcgggaata gaaccatgcg cggtaagaat
    42961 aattaccggc attcccggct gcactttctg gatttcagca aacagctgca taccgtccat
    43021 ttcatccatc cgcaggtcgc tgatgactaa atctactttt tcgcgattca gtacccgtaa
    43081 tccttcagcg ccactttccg ccgtgaccac gctgtagcct tcgctggtca ggcgcaggcc
    43141 aagcagtttc agcaatcccg gatcgtcatc gaccaataat aaatgcgcag gtttatggct
    43201 catcaggagt gacctcatgg gtggatggcg cgggcttttc actctcgtgt ggcgtatccg
    43261 gcgagaaatt tccagccggt ttgcgggtcg agagctggcg ttcaatatca gtcaggtttt
    43321 ccagcttgcg ggtggtgagt tccagttgct gctgtagaac gtgatgttgc tggcgcaatg
    43381 tatccagctc gctgtcgctg gactgctgga gtttactgta gcgttggcgc tcttccgcca
    43441 gttgcagttg cagcgcctga ccgtcgcgcc agagttgata cagtgggcga acctgtgccg
    43501 ggatctcggt acttaacgct tcaatacgcg cgaccagctg gcggcgctca tatggcgtaa
    43561 ttttggcgtc ggcgagcaaa atcccttgtt taaaggtatt ttgccagctg ccgtcgtcat
    43621 attggcgagc ttgctgacgc gactgcgcag gcattaaacg atcagcacaa tccatcgccc
    43681 gcagccagta aagcggattg gtttcggttg atttaccttg cagcgcccag atgtcgctac
    43741 attcagtaga aagatagtca gccagttgat aaaccggaat tttttcttct gctggcgtat
    43801 caatggctgg cttattgtga ttctgcacgc aacctagcaa tgccagacat ggcagccctg
    43861 ccagccacag ccgtcggggc aataatcgtt gaaaaatgtg tcgcatattc accagactta
    43921 aagcctatcc cagtgggcgt aattgttgca gacagtttgg acatggacag cgcggagaaa
    43981 ccggagcgta catgtcgtac gtgaggattt cgagcactgc ccggggccga actgacaaat
    44041 aaaatagcct gatgggatag gctgttagca ttatttcgtg tttttcgacg acggtaattc
    44101 aatgcggaaa caaacgtctt gcccgctctc gtcgaccaga tacagttccc cttgcatacg
    44161 gcgaatacaa tccctggcaa tgcttaatcc cagaccgctg cccttcaccg ccccttttcg
    44221 ctggtggctt ccctgaaaaa agggttcgaa gatcatggcg cgttcctctt gcggaatggg
    44281 cgtgcctgta ttgatgacat caatataaac ccgcgcacca tgtaaactgc tgcgaaggca
    44341 aatgttaccg gattcagccc cgtagtgcac cgcattggag taaagattat ccagtacgct
    44401 catcagcagc attggctccg ccaggcaagc tgttgctttg agatcgacgt cggtatgcat
    44461 cattttagcc cgtgcgggca ggctatgagc agaaactact gtctccacca gcggtgctaa
    44521 ctcaacatta tctagttcca ccgcactgtc cgcctgttta cggttgtaat caagcagttg
    44581 ttcgatcagt ttttgcaaat tgcggctgct gctatcaaga atgctcacca cctctttttg
    44641 ctctggcgta agcggcccga caacctgatc agccagtaat tcagtgccct cgcgcatact
    44701 cgccagtggc gtttttaatt catgagataa atgtcttaaa aattgatggc gttgggattc
    44761 cagccatgac aggcgctcac ttaaccaaag aatacgttgc ccaaccgagc gtaactcgct
    44821 cggtccactg aacgaaacgc tattgcccag agaacgccct tcccccaacc gattgatcat
    44881 ccgttcaata tttttcaccg gcccgataat catccgcgtg aaaagcagta ccattaccag
    44941 actcaccaga aatagcacca gcgattgcca accaaaatat tgcccacgtt cggcgatttc
    45001 acgctgaagt tgctgcccac gagagaacac tactgtgcgc gtggcctgta ccatttcggt
    45061 attggcactg gcaaaggctt ccagacgcgc ggcagcagca gcatcgggac cactgttgtt
    45121 gcactgaagt tgggccagat tgttcaagtc ctgacgcaat gcctggtaaa gtttatcgtc
    45181 tggcagtacg cctgcgtggg catcgagcat ttcgctgtaa cgcttgcgct ggctttgata
    45241 aaccttcgca agcgtagggt catccagcac gcaatactga cggtagctac gctccatctc
    45301 cagcgccgcg ttggtcattg cttcgctgcg ccgggcatcg ataagcgtgg tgcggttgac
    45361 cagcgccgcc tggtcgctaa gcgcattcag gctttgccag gcttgccatg ccagcaccaa
    45421 caggggcagc agaatcagca aaaatgccag cattaccagt tgtcgtaatg agcggggaaa
    45481 aacgggccag cgtttcaagg tgttactctc gtcagacgcg aatagcctga tgctaaccga
    45541 ggggaagttc agatacaaca aagccgggaa ttacccggct ttgttatgga ataaggcggt
    45601 gcctaactcg acgtttcgcc cgatggttga tatagctacg ctgatatcag aagttggacg
    45661 gcaggcacct tgttgtgcgt cattcgaatt ttatgtagca cgtcccgaag gggctgacat
    45721 aagtcggtga atgagccact gattactatt atgcagtaac tgtgccaata aagaaaatag
    45781 tttggtaacg tactgattat acgttgcttt gaggggttta tggcttcccc gctatttgaa
    45841 tgtaaatcag ccactgtgtc gctaaaaaga gacaacttaa gaaaaactta ttagattaca
    45901 ataaaatcaa tcatttaagt gtcgccagaa agcgacacgg taaaccaccc attgtcgtga
    45961 tttacagaca caaaaaagct cccgaagttg ggagcttata atagcggttg tatttaatgc
    46021 cggatgcggc gtgaatgcct catccgggct gcaaaaccaa tgagtttgac ggcttacccc
    46081 aactgcttac gtgcattgcg gaaaatgcgc atccatgggc catcctcgcc ccagttttca
    46141 ggatgccagg agttgctgac agtacggaaa acacgttccg ggtgcggcat cataatggtg
    46201 actcgaccgc tttcagtcgt gaccgccgta ataccgttcg gagaaccgtt cgggttagcc
    46261 gggtaggttt cagtgacttt gccgaagtta tcgacatagc gcagtgccac cagccctttg
    46321 ctttccagtg ccgccagatg cgccgcatca cgcacttcca cgcgcccttc accatgagag
    46381 acagcaatcg gcatttgcga gcccaccatc ccctgcaaca gcagcgacgg gctttgggtc
    46441 acttcaacca agctgaaacg cgcttcaaag cgatcggagg tattgcgcac aaaacgtggc
    46501 cacaactcac tacctgggat cagttcacgc agattagaca tcatctggca accgttacat
    46561 acccccagcg ccagcgtttg cggacggtgg aagaaggttg caaactcatc gcgtacacgg
    46621 tcattgaaca ggatagactt cgcccaacct tcaccggcac ccagcacatc accgtaggag
    46681 aaaccaccgc acgcgaccag ggcgtggaaa tcctccaggc ccgtgcgtcc agccagcagg
    46741 tcgctcatat gcacgtcgat agcatcgaag cctgcacggt ggaaagctgc cgccatttca
    46801 acatgcgagt tcacgccctg ctcacgcagc acagcaactt tcggacgtgc gccagtggca
    46861 atatacggtg ctgccacatc ttcgttgata tcaaacgaca gtttcacatt caggcccgga
    46921 tcggcgtcgt ttgatttcgc ctgatgctcc tgatcggcac actccgggtt gtcacgcagg
    46981 cgctgcatct gccaggtagt ttctgcccac cagacacgca acgtagtgcg gctttcgctg
    47041 aatacagtct gcccgttggc ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca
    47101 taatggacgc aatcagcaag cccatgctgt gccagtacgg actcgaccgc ttcacggtca
    47161 gcggcacgaa cctgaatgac cgcacccagt tcttcgttaa acaacgccgc caggcgatcg
    47221 tcacccagag tggcgatatc cgcgtcaatg ccgcaatgac cagcaaaggc catttccgcc
    47281 agcgttacca gcagaccgcc atcagagcgg tcgtgatacg ccagcaactt acgctgtgca
    47341 accagcgcct gaatcgcgtc atagaagcct ttcagttgcg cgacatcgcg tacatctgcc
    47401 ggtttgtcgc caagctgacg ataaacctgc gccagcgccg ttgcgcccag cgcgttattg
    47461 cctttgccca aatcaatcag cagcagtgcg ttatcttcgg tagaaagctg cggcgtgatg
    47521 gtgtgacgta catcttccac gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc
    47581 atttcgcgct cttcgttacc ttcctgccag cgggttttca tcgacataga gtctttaccc
    47641 accgggatcg tcaggcccag cgccggacaa agctcttcgc ccacggcttt aacggcttca
    47701 tacaggcccg catcttcacc agggtggcct gccgccgcca tccagttggc ggaaagttta
    47761 atgcgtttga tatcgccaat ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc
    47821 agacgggcag aggcggcgaa atccagcagc gcaaccggcg cacgctcgcc aatcgccatc
    47881 gcttcaccgt agtagctgtc gaggctggcg gtagtgaccg cgcagttagc gaccggcacc
    47941 tgccacggcc ccaccatctg atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg
    48001 gtcaccagga aagttttctc cgccacggtc ggcaaatgca gtacgcgttt caccgcgtct
    48061 gcaatggtga tcccttcacg gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg
    48121 cgggtcatct tcggcgtttt accaagcagg acgtccagcg gcagatcgat cggctgatta
    48181 tcaaaatgac gatcgtgcag agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg
    48241 tagggtgcgc gctcacgctt acacagttcg tcaaacagcg gtaattgatc ggcagcaacc
    48301 gccagcacgt agcgttcctg ggattcgtta caccagattt ccagcgggct catgcccggt
    48361 tcgtcgctta gaatatcgcg cagttcaaat ttaccgccgc gcccgccgtc gctcaccagt
    48421 tccggcatcg cgttagaaag accgccagcg ccgacgtcgt ggataaacag gattgggttg
    48481 gcatcaccaa gctgccagca acggtcgatc acttcctggc agcgacgctc catctccggg
    48541 ttgtcgcgct gtacggaagc aaagtcgagg tcggcatcag actgacctga cgccatggaa
    48601 gacgccgctc cgccgccaag accgatgttc attgccgggc cgccgagaac gaccagtttc
    48661 gcaccgacgt tgatctcgcc tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc
    48721 gccagcatga tcggtttgtg ataaccgcgc agctcttcgc cgttgtggct gttcactttt
    48781 tcttcataag tacggaagta gccattcagt gccggacgac caaattcgtt gttaaacgcc
    48841 gcgccgccca gcgggccttc ggtcatgatg tccagcgcgg tgacaatgcg ctcaggctta
    48901 ccgaaatctt cttcccacgg ctgttcgaag ccaggaattc gcaggttgga aacggagaaa
    48961 ccaaccaaac ccgctttcgg cttcgcgccg cgcccggtgg caccttcatc gcggatttca
    49021 ccgccggaac cggtcgccgc gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt
    49081 tcgactttca tcagaatatg cgccggttcc tgatggaaat cgtagcggcc cgtttcgtgg
    49141 tcagcaaagt agcggcccac ttcagaacct tccattacgg cggcgttatc tttataagca
    49201 gagagaacgt gatctggcgt ggtttcgaaa gtatttttga tcatcttgaa cagcgatttc
    49261 ggctgctgtt caccgtcgat aatccagtcg gcattaaaaa ttttgtggcg gcagtgctca
    49321 gagttcgcct gagcgaacat atacagctcg atgtcgttcg ggttacgacc aagctttgtg
    49381 aaagcatcct gcagatagtc aatttcatct tccgccagag ccaggccaag acgcaggtta
    49441 gcgtcgatca gcgcctgacg gccctgcccc agcaaatcaa cgctggtaac cggagtcggt
    49501 tgatggtggg caaacaactg ctctgcatca tctaaagcaa aaaagaccgt ttccatcatg
    49561 cggtcgtgca gttcagcggt aacctgctgc cattgttcat tggtcagcgt accggcttct
    49621 atatagtaag caacgccgcg ctcaaggcgg tttacctgtt gtagcccgca gttatgagca
    49681 atatcggtcg ctttcgaaga ccagggagag atggtgccag gacgcggggt caccagcagg
    49741 agtttgcctt gcggggcgtg gctggcgagt gccgggccat atttcagcag gcgttcaagt
    49801 tgtgcgtgct catcatcgtt taacggcgca ttgaggtcag caaaatggac atactcggcg
    49861 taaatattgt gaaccgggag cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg
    49921 aatgccgaca gtgcaggcga accacgcaga atttccatca taagtctctc gtcttctaag
    49981 ctttcggtgt acccaagggg ggaaacgggt gtcattataa agaatctgat gcgctgacga
    50041 aaccgtttgc gtggaaataa aatcaccatc gtgaattagc aacgcgtgcc gccaatggct
    50101 gtaataagtt gccatctggc gcaggtttac gcaaaatgcc gctcatttat gagtaaacct
    50161 ttcactatta ttacattttt tcaagctggg acgcgcacga cacagagaat taactaattg
    50221 aaaaaattaa agattaatta tctgttcatc ggcattctgg cactgctgct cgcggtcgct
    50281 ctctggccat ccattccctg gtttggtaaa gccgacaacc gtatcgccgc cattcaagcg
    50341 cggggagagt tgcgtgtgag caccattcat actcccctga cgtataacga aatcaacggg
    50401 aaaccttttg gcctggatta cgaactggcg aaacagtttg ccgattacct cggcgtaaaa
    50461 ctgaaagtga ccgtgcggca gaatatcagc cagctgtttg acgacctcga taatggtaac
    50521 gccgacctgc tggcggcagg acttgtctat aacagtgagc gggtaaaaaa ttatcagcct
    50581 ggccctacct attattccgt gtcacaacaa ctggtttata aagtgggtca gtatcgccca
    50641 cgtacgctgg gcaacctgac ggcggagcaa ctcaccgttg caccgggtca tgtggtggtt
    50701 aacgatctcc agaccctgaa agaaacaaaa ttcccggaat taagctggaa ggtagacgac
    50761 aaaaaaggct ctgcggaatt aatggaagat gtcatcgaag gaaaactcga ttacaccatt
    50821 gctgattctg tcgccatcag cctgtttcag cgcgttcacc cggagctcgc cgtagcgctc
    50881 gatatcaccg atgaacaacc ggtgacctgg tttagcccgt tagatggcga taataccctt
    50941 tccgccgccc tgctcgactt cttcaacgaa atgaatgaag acggtacgct ggcacgcatt
    51001 gaagagaaat acctggggca tggcgatgat tttgattacg tcgatacgcg cacattttta
    51061 cgcgccgtcg atgcggtact gccgcagtta aagcccctgt ttgagaaata cgccgaagaa
    51121 attgactggc gtttgctggc cgctattgct tatcaggaat cgcactggga tgcacaggcc
    51181 acttcaccga cgggtgtgcg cggcatgatg atgttaacca aaaataccgc gcaaagcctc
    51241 ggcattacgg atcgtaccga tgccgaacag agcatcagcg gtggcgtgcg ttatttgcag
    51301 gatatgatga gtaaagtgcc ggaaagtgtg ccggagaacg agcggatctg gtttgccctc
    51361 gctgcgtaca atatgggcta tgcgcatatg caggatgccc gcgccctgac ggcaaaaacc
    51421 aaagggaatc ctgacagttg ggctgacgta aaacagcgtc tgcctttact tagccagaaa
    51481 ccctattaca gcaagctgac ttacggctac gctcgtgggc atgaagccta cgcttatgtc
    51541 gaaaatattc gtaagtatca gattagcctg gtgggttatc tgcaagagaa agagaagcag
    51601 gctacagaag cggcgatgca actggcgcag gattatccgg cggtatcgcc tacggagttg
    51661 ggcaaagaga aatttccttt tctctcgttt ctttcccagt cgtcatcaaa ctatttgacc
    51721 cattctccct ctctgctgtt ttccaggaaa gggagtgaag agaaacaaaa ttaatccgtc
    51781 gaggattgcg cttttttctg cgctttaatt tcctggcggc gcatgcgaaa gaagtcactg
    51841 agcaacgccg cgcactcatc cgccagtatt ccttccgtaa tttccactcg gtgattcata
    51901 cccggatgat gcagcacatc cattaaagat cccgcagcgc cagttttcgc gtcacgcgca
    51961 ccaaagacca cgcgaccaat gcgactgtgg atcatcgctc cggcacacat tacacatggt
    52021 tcaagcgtga catacaacgt ggcgtcgatc agacgataat tttgcatcac cagaccgccc
    52081 tgcctcaggg ccatgatttc tgcatgtgcg gtgggatcat gtcgaccaat cggacggttc
    52141 cagccttcgc caataacacg attgttatgc actaataccg cgccgaccgg tacttcccgc
    52201 tcatcccagg cacgcttcgc cagcgtcatt gcatgacgca tccagtattc gtggctaaat
    52261 tcgacttcag acaaaaagaa aactccggtt ataaaagcgc ggcgcattat acacggacgc
    52321 tatgctttac tctattccag ttgctggagt tcaccgcgcg gggttactcg ccagcgatgc
    52381 tggcaaaagt aaagcagcgg gttgtcctga ttactgtcgc tatagccact gtacagccgc
    52441 agcggcgtgc cgattttgcg ctccagttgc gcgacctttt catgcccgag gcaacgcatc
    52501 gtcaataccc aaccaccata gccacgctga atttggctgg cgataagatt cacccgcggc
    52561 agccagggtg tatcgaaata gaccgcttca accagcggct gtggagaacc ggtaatcaac
    52621 cagatatcag catcggaact taacaggtag gtggttaatc gctcctgaac cagcggaaag
    52681 gcggtaacat tgtcacgaaa ccagcgtacg aaatcggcct gcaacgcctg taaacgtgct
    52741 tcgctgtgac caaaggtgca tccccacaga agcagactca tcggccagcg tgccgcacga
    52801 ccttttatca ataacgcaat ggctataatc ggcaacaacg gcaggacaag taacgcattc
    52861 agcggttggc gacgcagtaa atagcgcaga aaactgccga acatatcctg ctgatgcaat
    52921 gttccatcta agtcaaaaaa caccacacga cgctcgtgag ttgccaaacc atactcctct
    52981 ggactgaaac atcctgatta attttcgcta ctacatagcc taacagatag atcatcactt
    53041 ttccggcaac aatcgaccgc atcatggcta actggaattt ttaattcatg gcaattagcg
    53101 gcaatggaat ataaaattca ctcgcgtgtg tctcatattg cctgttgtcg ccactatttc
    53161 atcgcgagga aagatatgaa cggcttactt cgtatccgtc agcgttacca ggggcttgcc
    53221 cagagtgata aaaaactggc ggattatctg ctgctacaac ctgatacggc gcgccattta
    53281 agctctcagc aactggccaa cgaagccgga gtcagtcagt ccagcgtcgt gaagttcgcg
    53341 caaaaactcg gctataaagg ttttccggcg cttaagttgg cgttgagtga agcgctggca
    53401 agccagccgg aatcgccctc agtgcccatt cataaccaaa tccgcggtga tgatccgtta
    53461 cggctggtcg gcgaaaaact gattaaagaa aatactgccg ctatgtacgc aacgctaaac
    53521 gttaatagtg aagagaaact gcatgaatgc gtaacaatgt tgcgctctgc gcggcggata
    53581 attctgaccg gtattggcgc ttcgggtctg gtggcgcaaa actttgcctg gaagctgatg
    53641 aagattggct tcaatgctgc cgcagtgcgc gatatgcatg cgctactcgc taccgtacag
    53701 gcgtcgtccc ctgacgatct gttattagcc atttcttaca ccggtgtacg acgcgagtta
    53761 aacctggcgg cagatgagat gctgcgagtg ggcggaaaag tgctggcgat taccggcttt
    53821 actcccaatg ccctgcaaca gcgtgcttct cattgcctgt ataccattgc cgaagaacaa
    53881 gcgacaaaca gtgcttcaat ctctgcttgt cacgcgcagg gaatgttaac ggatttgctg
    53941 ttcattgcgc tgattcagca ggatctggaa ctggcaccag aacgtattcg tcatagtgaa
    54001 gcgctggtga aaaaactggt ctgagtaaag aatgcgcgta taatgcccgc ccggtttgtg
    54061 ttgttttgag agtttcctta tggcgctgtt aatcactaaa aaatgcatca attgtgatat
    54121 gtgtgaaccc gaatgcccga atgaggcgat ttcaatggga gatcatatct atgagattaa
    54181 cagcgataag tgtaccgaat gcgtagggca ctacgagaca ccaacctgcc agaaggtgtg
    54241 cccgatcccc aatactattg tgaaagatcc gcaacatgtc gagacagaag aacagttgtg
    54301 ggataaattt gtgctgatgc accacgcgga taaaatttaa tattctactc tggaagtaga
    54361 gtattaatta ttttactggg aagccttaac gccattatat ttatttaatt gatgacatta
    54421 gcataataat tcactaagtt aatttatata gtatctgccc agacacttat ttatagttat
    54481 taaaggcgcg tccgattggt tcaccggacg caccttaagt acgtttcctt gtgttataag
    54541 aacagaagga tcagctgtaa aacagcaatg atgattttga tgacccgttt aatcaggtat
    54601 cggcaatcag tcattcgttt ttccttaaac aaggaatgca gccgttcaga ttagccctta
    54661 catctcccca aaactgaacg tgcgagttat tggaatcgag tgctgcactc cacaccagag
    54721 ctttgacgac accactcgtt tcaatggggg aattcagtgg catggtgtaa agcacagcaa
    54781 aattgccaat aacgaaaccg attttaatgt acttatcaaa aagcggtcac gttaatcttt
    54841 tcatcaatgt cgatttcttc gtaaaggctc gcgtttttta tgtcagagat tgcaagttgc
    54901 ttgtacaggt caacgaaatt gatccataat cattttcgtt ggaatcgtgt gctgcacaaa
    54961 attaaagtta aaaagtaaaa cccccgttcc ttaccagttc gggggtttta ctttataaag
    55021 agaacggtat tatttttaac tttcaataat taccgtggca caagcatagt ggcgctcatc
    55081 ggccagcgtc acatgcatat ttgcaacgcc cagcttttcc gccagtttta atgcctcgcc
    55141 ccacagtcgc agccgtggct taccaagctc atcgttaaat acttcaaact gattaaacgc
    55201 cagaccattg cggatcccgg tgccaaacgc ttttgctgcg gcttctttta cggcaaagcg
    55261 tttcgccaga aaacgcaccg gctggtggtg cgttttccaa atttcccatt cgttatcgct
    55321 taatacgcgg cgtgccaggc gctcaccgga tcgggcgatc actgcttcta tgcgagcgat
    55381 ctctacgata tccgtgccta aacctaatat tgccattagc cacgcgcttc cagcatcagg
    55441 cgcttcattt ctgccaccgc atctttcagt ccggtcatca ctgcacgacc aataatggca
    55501 tgaccgatat tcagttcgtg catctcaggg atggcggcaa tggcttttac gttgtgatag
    55561 gtcagaccgt gtccggcgtt aactttcaaa ccgagactag cggcaaaggt cgcggctttg
    55621 gcgatacgcg ccagctcttg cgcctgttcg gcgtcagttt tggcatcagc atagcaaccg
    55681 gtgtggatct cgataaacgg tgcgccaacc tctgccgcag ctttgatctg ctcttcatcg
    55741 gcgtcgataa acagagaaac ctgaatcccg gcatccgcca gacgtttgca ggcatcgcgc
    55801 attttgtcac gctgccctgc aacgtccagg ccgccttcgg ttgttacttc ctgacgcttt
    55861 tctggtacca ggcagcaaaa atgcggcttc gtctcaaccg cgatcgccag catctcttcg
    55921 gtcaccgcca tctccagatt catgcgggta tccagcgtct gacgcaggat gcgcacatcg
    55981 cggtcagtaa tgtggcggcg atcttcacgt aaatgcacgg taatgccgtc cgcccccgcc
    56041 tgctcggcaa taaacgcggc ctgcaccgga tccgggtaag cggtaccgcg cgcgttgcgc
    56101 agcgtagcga tatggtcaat gttgacgcct aacagtaatt cagccatgac aatcctcatc
    56161 attcataatg tgttttcacc gttcgcttag gcataaattg ccggaacagt tccctgctct
    56221 ttaaaggttt accgccaaga tacggcttaa gcgccatgcg ggtaaagcgt ttcgcggcgc
    56281 gcagtgtgtc tgcgtcagga aattcccgtg cgtttaacgc ttttaactgc cttccggtga
    56341 acgttttatt gtcgataacg acgcttgcga taaacccttt ttcttcgcga taacgatacg
    56401 tcatggtgtc atctaccggc tcgccgctac ccgcacaatg ggtaaaattg acgccataac
    56461 ccagatgccc gagcagtgcc agttcaaagc ggcgcagcgc gggttctggc gtaccagtga
    56521 cccctgcaag agactgaatg cagtgcaagt aatcgaaaaa gagttcagag aagcgcgtct
    56581 cgtattccag tacgcgggag agaagttcgt tgatgtacag accgctgtaa agcgtgatac
    56641 cgcttaatgg cagcgccagc gagacagctt cagcactgcg cagcgttttg acttcgccac
    56701 gcccgccaaa acgtagcaag agaggggtga aaggctgtaa tgcacctttc agggtagagc
    56761 gtttagagcg tgcgcctttg gcaaccagac gcacgcgacc cgattcctcc gtgaagacgt
    56821 ccagcatcag gctggtttcg ctccacgggc gactatgcag gacaaatgcg cgctgccagc
    56881 cttccatcgg agttactctt aaagatcgtc aacgtaaccg agactgcgca gtgcgcgttc
    56941 gtcgtcggcc caaccggatt tcacttttac ccacagctca aggtgaacag gcgcttcaaa
    57001 catttcctgc atgtctttac gcgcttcaat cccgatggtt ttgatcttgg cccctttgtt
    57061 gccaatgacc atcttcttct gcccttcacg ctcaacgaga atcaaaccgt tgatgtcata
    57121 accaccgcgt tcgttagaga cgaaacgttc gatctccacg gtcacggagt acggcagttc
    57181 agcgccgagg aaacgcatca gtttttcgcg gatgatttca gacgccataa aacgctgtga
    57241 gcgatcggtg atgtaatctt ccgggaagtg atgagtcgct tcaggtagat gcttacgcac
    57301 gattgccgca atagtggcaa cattcagccc ggtttcggca gagattggca cgatatcgag
    57361 gaagttcatc tggcttgcca ggaactgcag gtgcggcagc agatcggctt tctcctgcac
    57421 gttgtccact ttgttcaccg cgaggattac cggcgctttg ccttcgcgca gtttgttgag
    57481 caccatttcg tcgtccggcg tccagcgggt gccttcaacg acaaaaatca ccagctcaac
    57541 atcgccaata gagctgctcg ccgctttgtt catcaggcgg ttaatagcgc gtttttcttc
    57601 catatgcagg cccggtgtat cgacgtagat cgcctgatac gcgccttcag tatggatccc
    57661 cacaatgcgg tgacgagttg tctgcgcctt gcgggaggtg atggagattt tctgccccag
    57721 cagtttgttc aacaatgtgg atttgccaac attcggacgt ccgacgatgg caataaatcc
    57781 gcagtaactt ttatcgatgc tcattccagc tccagttttt tcaacgcctg ttcggcggca
    57841 gcctgctcag ccttacgacg gcttgaacct gtgccaacca ccggttcact caggccgctg
    57901 acctggcagt ggattgtaaa ttcctgatcg tgcgcttcgc cacggacctg aactaccaga
    57961 taagtcggca gcggcagatg acgaccctgc aaatattctt gcaagcgcgt tttcggatct
    58021 ttttgtttat cgcctgggct aatttcgtcc agacgagttt gataccagtt gaggattaat
    58081 ttctcgacgg tttgaatatc actgtcgagg aatacgccac caattaatgc ttcgacggtg
    58141 tcggcgagaa ttgactcacg acgaaatcca ccgcttttaa gttcacctgg tcctaaacgt
    58201 aagcactcgc ctaactcaaa ttcgcgcgcc agttccgcca gcgtattgcc acggaccagc
    58261 gtggcgcgca tccggctcat atcgccttca tccacacgag ggaaacggtg ataaagcgca
    58321 ttggcgataa cgtagctcag aatagagtcg cctaaaaatt ctaaacgctc gttatgtttg
    58381 ctgctggcac tacgatgagt taatgcctgc tgcaacagtt cctgatgatt aaaagtgtag
    58441 cccagcttcc gttgaagccg attaattacg atggggttca tgcgatacca ataaataaat
    58501 gcgtcaacaa ttcagcacac gaaacagacc tgatatacat ggctctgcta actgcttcgc
    58561 tgcagtttca gtttagcggt atatggggac caacgctgtt tcgtgtgccg tggcaacctg
    58621 gaggtgccaa ccttaaactt cgggggaata ttctatacac aacgacgggg gatgtcgtta
    58681 gccacgggag atttatctca taaataattc acgttgtcgc cataacggcg acaacgtgaa
    58741 cgaagatgag ctattaatgg atgccgccaa tgcgacttaa gcgcacacct gtcggccatt
    58801 cgccttcttg cttatcgaag ctcatccaga tagccgttgc ccgaccgacc agattcgctt
    58861 caggcacaaa gccccagtaa cggctgtccg cgctgttgtc gcggttgtcg cccatcatga
    58921 agtattgtcc cggaggaaca atccaggttg ccagttgttg ccctggctgc tggtaataca
    58981 tccccacctg atcctgcgca atcggcactg tcagaatgcg gtgcgtcaca tcacccagtg
    59041 tctctttacg ctcggaaaga cgaattccat tttctttggt ttcgtttttc ggcacttcaa
    59101 agaatccgct ggtcgcttcc ccaccattac ggcgtgagaa ggtctgaacg aaatcgctcg
    59161 gttccacgtt tgagtaggtg accggcagcg cgttttcaca cgcctggccg gaactgcatc
    59221 ccggttgaat cgtcagctct tttgagaccg gatcgtaagt gactttatcg cccggtaaac
    59281 ccaccgcgcg cttgatataa tcaagctttg gatcttccgg atatttaaag accacgatat
    59341 cgccgcgttt cggatgaccg gtttcgatca gcgttttctg gtagatagga tctttaatgc
    59401 cataagcaaa cttctctacc agaataaaat caccgattaa cagagtcggc atcatcgaac
    59461 ctgacggaat ctggaacggt tcataaataa acgaacgcac aatcaatacg atagccagta
    59521 ccggaaaaac agaagcaccg gtttccagcc agccaggctt cggcgcaacc tttttcaacg
    59581 ttgctttatc cagtgagtca ccggcagccg cctgcgccgc tgcctgacgt tcccgccgtt
    59641 taggtgcgaa aaagaattta tccacgcacc ataaaatgcc cgtcaccagt gtggcaatca
    59701 ccagaatcag ggcaaacata ttcgccatgc caactcctaa gggttatttg ttgtctttgc
    59761 cgacgtgcag aatggcgagg aacgcttcct gcggcagctc gacgttaccg atctgcttca
    59821 tgcgtttctt accttctttc tgcttctgca gcagcttttt cttacggctg atatcgccgc
    59881 cataacattt agccagtacg tttttacgca gctgtttcac ggtggatcgc gcaataatgt
    59941 gcgtaccaat cgctgcctga atggcgatat caaactgctg gcgtgggatc agatctttca
    60001 tcttctccac caactcgcga ccgcggtttt gcgaattatc acggtgggtg atcaacgcca
    60061 gcgcatcaac acgttcaccg ttgattaata cgtctacacg taccatgtcg gacgcctgga
    60121 agcgtttgaa gttgtaatcc agagacgcat aaccacgcga ggtagatttc aggcgatcga
    60181 agaagtcgag caccacttcc gccatcggga tctcgtacgt cagcgccacc tgattaccgt
    60241 ggtaaaccat attggtctgc acgccgcgtt tttctacgca cagcgtaata acgttgccga
    60301 gatatgcctg cggcagcagc atgtgacact ctgcaatcgg ctcgcgcagt tcgtaaatgt
    60361 tatttaccgc aggcagcttg gatgggctgt cgacgtagat aacttccctt gacgtggttt
    60421 caacttcata cactacggtc ggcgcagtgg tgatcagatc cagatcgtat tcacgttcca
    60481 gacgctcctg gatgatctcc atgtgcagca ggccaaggaa gccgcagcgg aaaccaaagc
    60541 ccagcgcgct ggagctttcc ggctcataga acagtgaggc atcgttcagg ctgagtttac
    60601 ccagcgcgtc acggaaggct tcatagtcgt cggaacttac cgggaacaga ccggcgtata
    60661 cctgcggttt gactttctta aagccaggca gcgccttttc tgccggatta cgcgccagcg
    60721 ttaaggtatc gccgactgga gcgccgtgga tatctttaat cgcacatacg agccagccta
    60781 cttcgccaca tttcagttca gtgcggtcaa cctgtttcgg cgtgaagatg cccagacggt
    60841 cggcgttata ggtctgcccg gtactcatga ctttcacttt gtcgccctta cgcagggtgc
    60901 cgtttttaat acggataagt gaaacaacgc ccaggtagtt gtcgaaccat gagtcgataa
    60961 ttagtgcctg caacgggcct tccggatcgc cttccggcgg cggaatgtcg cgcaccagac
    61021 gttcgagaac gtcctgcacg ccaacgccgg ttttcgctga acagcgcacc gcgtcagtgg
    61081 cgtcgatgcc gacgatatct tcaatttctt ccgccacgcg ttcaggatcg gctgccggca
    61141 ggtcaatctt gttcagtacc ggcaccactt cgagatccat ttccatggcg gtgtagcagt
    61201 ttgccagggt ttgcgcttcc acgccctgcc cggcgtcgac caccagcaat gcaccttcac
    61261 aggcagccag cgaacgggaa acttcatagg agaagtctac gtggcccggg gtgtcgataa
    61321 agttaagctg ataggtttcg ccgtcagacg ctttgtagtc cagcgtcacg ctttgcgctt
    61381 tgatggtaat gccacgctca cgctcaagat ccatggaatc gagaacctgc gcctccattt
    61441 cacggtcaga caggccaccg cagatctgga taatacggtc agacagcgtc gatttaccgt
    61501 ggtcaatgtg agctatgatc gaaaagttac gtatattctt cataaagtat gattattgtg
    61561 ccttaatgcc cggttaacca ggcttttaga agtcgctgtt ctgagcttaa cgtctgtatt
    61621 aatagaaacg ccgcattcta cactacaaca ttgaggcgag gaaatgttca taccgtatgg
    61681 attgtggtat ctggaaacgt cctcgcattt gttatgcaaa atgcaacaaa gccagtgaaa
    61741 tcactggctc gcgtcttccg aagatgtttc aaatcgcaca aggccaggcg gcaatgccac
    61801 gcttaagatg atcggttgcc attctgcccg ggcagcaaac ttacgcgagt agccacgcgc
    61861 aatcaggaac ccgccaatac cgccgaggac cgcaccacat aatgctgcga cgtcagaagc
    61921 aaagagtagc tgaaatagcg aagcgatgag gaataatccc accagcggcg acatataaac
    61981 cagtaatgcg gagctaagca ggctgccttc ggcgatcccc aattccactt tttgccccgg
    62041 cactaacggc tcatcacagg gtacgacaat ggtatgcgtg gtttgcgggc caagtttatt
    62101 caacacgcgg ctaccgcaac cggcgcgtga agcgcagctg ctgcatgagg ctttaacatc
    62161 acaactgacc agcgcctgcc cgttttgcca ggagacgacg gtagcccact ctttgatcat
    62221 tgcgctgccc cgaacttaat attctcggca atgcgtttcg ccgtttgcgg cggcagttca
    62281 ccgacaatgg tgatttcggc gttatcacgt acgcttgtac tgacggttct gcgtccggtg
    62341 cgcaacatct gatcggtgct cgatggcgta gcgcggttaa cgtttaccga gaagctgaat
    62401 aatccgtcgg aatagagacg tgattcgata ggcatgttgt ccatcgtcgg tagcggacgt
    62461 cgactactgg aaacttcgct aaaaccctgt ggcaaccagg ttggcgtcca gctgaattta
    62521 gctttttcac ctacaggaac agaaagcaac ggcggcaaat ttgccttcgc cagcgtctgc
    62581 atactgctgc tgatatcctg attgacgtta aaagcaatca cgcgaaattg ttccagcgtt
    62641 tcaccatcgc gatcaagaag atcaacccgc atcggtaatt tcgattcggt gtccatccac
    62701 acgatgtagc tgtagcgtgt accatctcgg gcaaccacgc gaatgacttc gcaaagacga
    62761 tcagcaatac gcgtgcgccc gacggagata aagtcgtagt aaggagaaag gcgtttgaaa
    62821 tcggtataga tgagcgatgg cagagaatca acaatgtaat cgccattaag cgtgaacggt
    62881 tcaagtcccg gttcaaaata gctgatttca ttgccgcgct gtaccacttc ccggcgcggg
    62941 ccatccattt gcaacaattg tgcaagagga cggttatcga ggcgtgcatg tcgataacgc
    63001 agagactcaa caccctgttt attgatgctg atgaatgaca gctcgtaatt cagtgactga
    63061 ctggccaggt tcatctgctg taataacgcc ccggacgcgg gagtggccga ggcgttagca
    63121 gagaataaca ggctacctgt cactaatgac atggcaaacc aaagttgctt cattactgcg
    63181 attgcgttcc taaagtttga attcctggca cctgtacagc ggcttgctgg gtttgcgcct
    63241 gctcaaactg aagctgttca gagtggagtc ggcgttgcag ttcgtaatcc tgcaacattg
    63301 cattaatgcg acgacgctgc tgctgttgac cattatttgc ggtcgcttca gaaggtactc
    63361 ccaggctaac cgggctggct ttacccatca tcggcagtgt attaaatacc ggcgtttcgg
    63421 gctgctggga cgtttcagat tgtccattat agtgctggac gccaacgata actgcaagcg
    63481 atacgcatgc ggctacgccc atttgggtaa gctgtgccgc ccacggacgt actttctgcc
    63541 agaatggcat tttctgccat tcatgcggcg caggctgggc ttccgggatc aatgtcgccg
    63601 gttgacgtac tggctcttct tcaatggcgg ccatcacgcg tgaagagata tcgaaatgga
    63661 gcacctcggg agtatcaccc cgcattgagt cacggattaa gtgatagctt tcccaggttt
    63721 tctgcatttc tgggttatga gccagttcgt taagcagctc actatccagc gtttcgccat
    63781 ccattaaagc ggaaagttgt tctttctgca tgcctaatac ccttatccag tatcccgcta
    63841 tcgtcaacgc ctgataagcg gttgaacttt gttatcaata gcttccctcg ctcggaagat
    63901 acgtgaacgc accgtaccta ccggacaatc catgatagcg gctatctctt catagctcag
    63961 gccatccagc tcccgcaagg ttattgccat gcgtaaatct tccgggaggg actcaatagt
    64021 tcggaaaact atctgtctca gttcttctga caacattaag ttctcagggt tcgaaatttc
    64081 tttcaacgcg ccgccacttt cgaagttttc agcttcaatg gcatccacat cactggaagg
    64141 tggacgacgc ccctgagcaa ccaggtaatt tttcgctgta tttacagcaa tccgatacag
    64201 ccatgtataa aaagcgctat ctccccggaa cgaatccagc gcacgatagg ctttaataaa
    64261 agcttcttgt accacatcgg gaacatcacc cgacggcaca tagcgggaaa ccagactcgc
    64321 cactttatgc tgatagcgca ctaccagtaa gttaaaggct ttctgatctc ccttctggac
    64381 ccgttcaacc aggacctggt ccgttaactg ctcgctcatc cgaggtaaag tctccccaaa
    64441 ccaaatttcc acgcgctatc gaaacgccac tccattagct gcaatttgag caagcaaagg
    64501 gttagagtgt ctcgtttttg taaagttccg taacgcatct gtttttgttt gtcatgctgt
    64561 agacggatca ttatctatca ttataagtct acagaatctg aacatcgcat tatctgtgta
    64621 gaaatgccca tttaactgcc tgaagagtaa cccaacggcc tttttatttc accacctaat
    64681 cctccaccag ccagtaactt ctctttttct cgccgccctg cgtcagcgtg tttagtcact
    64741 gtaacaaata ttaaaatagc aggtgtttat tcgcacaaca tgatgctatg ctgaccaaac
    64801 cgtgtttagt aaattaaaca aagaaaatga atactctcct tgaacattca tgtgacgtgt
    64861 tgattatcgg tagcggcgca gccgggcttt cactggcgct acgcctggct gaccagcatc
    64921 aggtcatcgt tctaagtaaa ggcccggtaa cggaaggttc aacattttat gcccagggcg
    64981 gtattgccgc cgtgtttgat gaaactgaca gcattgactc gcatgtggaa gacacattga
    65041 ttgccggggc tggtatttgc gatcgccatg cagttgaatt tgtcgccagc aatgcacgat
    65101 cctgtgtgca atggctaatc gaccaggggg tgttatttga tacccacatt cagccgaatg
    65161 gcgaagaaag ctaccatctg acccgtgaag gtggacatag tcaccgtcgt attcttcatg
    65221 ccgccgacgc aactggtaga gaagtagaaa ccacgctggt gagcaaggcg ctgaaccatc
    65281 cgaatattcg cgtgctggag cgcagcaacg cggttgatct gattatttct gacaaaattg
    65341 gcctgccggg cacgcgacgg gttgttggcg cgtgggtatg gaaccgaaat aaagaaaagg
    65401 tggaaacctg ccacgcaaaa gcggtggtgc tggcaaccgg cggtgcgtcg aaggtttatc
    65461 agtacaccac caatccggat atttcttctg gcgatggcat tgctatggcg tggcgcgcag
    65521 gctgccgggt tgccaatctc gaatttaatc agttccaccc taccgcgctg tatcacccac
    65581 aggcacgcaa tttcctgtta acggaagcac tgcgcggcga aggcgcttat ctcaagcgcc
    65641 cggatggcac gcgttttatg cccgattttg atgtgcgcgg cgaactggcc ccgcgcgata
    65701 ttgtcgcccg cgccattgac catgaaatga aacgcctcgg cgcagattgt atgttccttg
    65761 atatcagcca taagcccgcc gattttattc gccagcattt tccgatgatt tatgaaaagc
    65821 tgctcgggct ggggattgat ctcacacaag aaccggtacc gattgtgcct gctgcacatt
    65881 atacctgcgg tggtgtaatg gttgatgatc atgggcgtac ggacgtcgag ggcttgtatg
    65941 ccattggcga ggtgagttat accggcttac acggcgctaa ccgcatggcc tcgaattcat
    66001 tgctggagtg tctggtctat ggctggtcgg cggcggaaga tatcaccaga cgtatgcctt
    66061 atgcccacga catcagtacg ttaccgccgt gggatgaaag ccgcgttgag aaccctgacg
    66121 aacgggtaat aattcagcat aactggcacg agctacgtct gtttatgtgg gattacgttg
    66181 gcattgtgcg cacaacgaag cgcctggaac gcgccctgcg gcggataacc atgctccaac
    66241 aagaaataga cgaatattac gcccatttcc gcgtctcaaa taatttgctg gagctgcgta
    66301 atctggtaca ggttgccgag ttgattgttc gctgtgcaat gatgcgtaaa gagagtcggg
    66361 ggttgcattt cacgctggat tatccggaac tgctcaccca ttccggtccg tcgatccttt
    66421 ctcccggcaa tcattacata aacagataaa aagcctgggt tagcgccgta tacgcttcgg
    66481 aatagttctg gtctggccca cgaatgacta agcgatcgct aaagcattct cccgcctgcg
    66541 gggagaatgc cagcagcacc cgatgcggca gtcgcgcttc gttttccgcc acatccgtcc
    66601 gcaaacgtaa atgccagccc atacttaatg ccagctccgt aaaactatta ccaatctgct
    66661 ctggcagcac cacgcagaaa aatccctctt cggtaatgca ctccgccgcg caagtaagta
    66721 aagtctggtg atcaagtgag gtggtgtagc gcgcctgttc ccgctgcggc gtcgcgcact
    66781 ccactccttg ctcataatac ggcgggttgc tgatgattaa atcaaagcgc gcggtctgct
    66841 gtgttaccca ctgctgaata tccgccgtat ggacgttaat tctctctaac cacggggact
    66901 gggcgacatt ttcctgcgcc tgagtggcag cttcactttc cagctcaacg gcatcaatta
    66961 tcacgctgtc actggttcgc tgcgccagca tcagtgccag caagccacta cccgcaccga
    67021 tatcaaggca acgttttacc ccagccaccg gtgcccatgc gcccagcaat atgccatccg
    67081 ttcccacttt catcgcacag cgatcgtgag caacaaaaaa ctgtttaaaa gtaaatccat
    67141 tacgacgaag cacggatgta gactgtgaca tgaaaataaa accttgcagg aaaaacggcg
    67201 atagcaccgg gtgagaacaa tacccgagaa gcgatatcca tacaaacaga tgaagattgc
    67261 agccgtaacg tctataatca gcgccccaca cagaggtaga acatgactgt aacgactttt
    67321 tccgaacttg aactcgacga aagcctgctg gaagccctcc aggataaagg tttcactcgc
    67381 ccgaccgcca ttcaggctgc cgccattccg cctgcgctcg atggccgtga tgtactcggt
    67441 tctgcgccga caggcaccgg taaaacggcg gcgtatctgc tgccagcgtt gcagcacctg
    67501 ctcgatttcc cgcgtaagaa atccggtccg ccgcgtattt tgatcctcac cccaactcgc
    67561 gagctggcga tgcaggtggc cgatcatgcc cgcgaactgg cgaaacatac gcatctggat
    67621 atcgccacca tcaccggcgg cgtagcctat atgaaccacg cggaagtgtt cagcgaaaat
    67681 caggacatcg tggtcgccac gaccggacgt ctgctgcaat acataaaaga agagaacttc
    67741 gattgccgcg cggttgaaac gctgatcctc gacgaagcag accgtatgct ggatatgggc
    67801 ttcgctcagg atatcgaaca tattgctggc gaaacgcgct ggcgtaaaca gaccctgctc
    67861 ttttcggcaa cgctggaagg cgatgcgatt caggactttg ccgagcgtct gctggaagat
    67921 ccggtggagg tttctgccaa tccgtccacc cgtgagcgca aaaaaattca tcagtggtat
    67981 taccgcgccg atgacctgga acataaaacc gcattgctgg tgcatctgtt aaaacagccg
    68041 gaagcgaccc gctcaattgt gtttgtgcgt aagcgtgagc gtgtgcatga gctggcaaac
    68101 tggctgcgcg aagcgggcat caacaactgc tatctcgaag gtgagatggt acagggcaag
    68161 cgtaacgaag cgatcaaacg tttgaccgaa ggtcgcgtaa acgtactggt tgcaaccgat
    68221 gttgccgcgc gcggtatcga cattcctgac gtcagccacg tctttaactt cgatatgccg
    68281 cgcagtggcg atacttattt gcaccgtatc ggacgtaccg cgcgcgccgg tcgtaaaggc
    68341 accgcaattt cgctggtaga agcccatgac catctgctgc tgggcaaagt tggtcgctat
    68401 attgaagagc caattaaagc tcgcgtaatt gatgagttac gcccgaaaac gcgtgcacca
    68461 agcgagaagc agaccggcaa gccatcgaag aaagtactgg caaaacgcgc cgagaagaaa
    68521 aaagcaaaag agaaagagaa gccgcgggtg aaaaaacgcc atcgcgatac caaaaatatt
    68581 ggtaagcgcc gtaaaccaag cggaacgggc gtgccaccgc aaacgacaga agagtaatct
    68641 caatgccggg tttaagcccg gcattatttt tatcccggca aaaaacaatc ttcaacacac
    68701 tgaataaaag tgtgcatcgc cgggctaacc gcttttccgg catggtgagc gcacattgcc
    68761 gtaatggtct gcgagtgttc gccaaaaggc aattcaatta attctccgct ctcaagctct
    68821 ttttccaccg caaagcgggg caagtaactg acaccgatat tcgccgcgac acaacgtttg
    68881 atactttcaa tgcttaacag ctcaatggtg ttttccaccg tgatccgccg ctggcgcagc
    68941 gtgctctcaa atatctgccg gaatacacat tgcggttcgt tgataataaa gctacaggcg
    69001 ttatgtcttc ccggctcagt aaaatcgaca tctgcaattt gcggtgaagc taccagcacc
    69061 agcgattgct cacccaattc tcgtcggttc agggcatcat cattccctac gcgataaaaa
    69121 accccgacat cggcctcatc attcaatagc gcatcacgga tcacgtaaca gttcagagac
    69181 tgcaacgata aacgcacttt tggggcgcgt tgccgaaaac gctgcaacac ctgcggcatt
    69241 cggtaagaga gcaacgtttc gcccgaaaca acgcgtagtt ccccgtccgg atccgactcc
    69301 tttttggcag cctcacgaag ggtatccatc actctggtga gttcataaat gtgcggcagc
    69361 agctttttac cttcgcgagt gaggcacatc cgtcggccaa ttttctcaaa taactgtacc
    69421 gagaactcct gctcaagctg ctgaatatga aaagtcaccg tcgattgtgt acagcacaat
    69481 ttttgcgaag ctcgcaaaaa ggaaccctct tccaccacgg ttttaagcgt aataaaacgg
    69541 cgcagatcca taaccccaaa cctatcgaaa atatcgaatt tagaatgtaa aaacattcat
    69601 ttttttaaat attctgtgtc gggtactgtc taccaaaaca gaggagataa caagtgacac
    69661 cgaccctttt aagtgctttt tggacttaca ccctgattac cgccatgacg ccaggaccga
    69721 acaatattct cgcccttagc tcagccacaa cgcacggatt tcatcagagt acccgcgtgc
    69781 tggcagggat gagtctggga tttttaatcg tcatgttgct gtgtgcgggc atttcgtttt
    69841 cactggctgt tatcgacccg gcagctgtgc atctgttgag ttgggcgggg gcggcatata
    69901 ttgtctggct ggcgtggaaa atcgctacca gtccaacaaa ggaagacggg cttcagacaa
    69961 aaccaatcag tttttgggcc agctttgctt tgcagtttgt gaacgtcaaa atcattttgt
    70021 acggtgttac ggcactgtcg acatttgttc tgccgcaaac acaggcgtta agctggatag
    70081 ttggcgtcag cgttttgctg gcaatgattg ggacgtttgg taatgtgtgc tgggcgctgg
    70141 cggggcatct gtttcagcga ttgtttcgcc agtatggtcg ccagttaaat atcgtgctgg
    70201 cgctgctgct gatctattgc gcggtacgca ttttctatta atgaaaaaaa gcggaagagg
    70261 tcgccctctt ccgcttagta acatgctact taagccttac aggctttcag taaaggtacg
    70321 agcgataacg tcgcgctgct gttccggagt cagagagtta aagcgaactg cataaccgga
    70381 tacacggatg gtcagctgcg gatatttttc cggatgctta actgcatctt ccagagtttc
    70441 acgacgcaga acgttaacgt tcaggtgctg accaccttca acgcgaactt ctggtttcac
    70501 ttctactgga acttcacggt actcaatgtc acccagtttg cttactgcaa ccacttcgtc
    70561 ttctgcataa cctgctttcg caacgatgca acgcgcttcg cctttttcgc tgtccagcag
    70621 ccagaaagag ttcagcagat cgtcgttagc ggctttagta atctggatac ctgtaatcat
    70681 gtgatgcctc cccggcaaaa ttatttgatt tgttcagcct gtcgcggcca attggtaaaa
    70741 ccattgttgc ttgagtgtat atatactcct caaacaccct tgaatctttg atttaaatca
    70801 ataaaaacca cacatcaagt gtggtcgcaa atagatttta ttgttttaca tcaacttatg
    70861 cgggtgtgaa attttaccaa tttacatttt tttgcactcg tttaagtcta aaaaatgagc
    70921 atgattttgt tctgtagaaa gaagcagtta agctaggcgg attgaagatt cgcaggagag
    70981 cgagatggct aacgaattaa cctggcatga cgtgctggct gaagagaagc agcaacccta
    71041 ttttcttaat acccttcaga ccgtcgccag cgagcggcag tccggcgtca ctatctatcc
    71101 accacaaaaa gatgtcttta acgcgttccg ctttaccgag ttgggtgacg ttaaagtggt
    71161 gattctcggc caggatcctt atcacggacc gggacaggcg catgggctgg cattttccgt
    71221 tcgccccgga atcgccactc ctccgtcact gctgaatatg tataaagagc tggaaaatac
    71281 tattccgggc ttcacccgcc ctaatcatgg ttatcttgaa agctgggcgc gtcagggcgt
    71341 tctgctactc aatactgtgt tgacggtacg cgcaggtcag gcgcattccc acgccagcct
    71401 cggctgggaa accttcaccg ataaagtgat cagcctgatt aaccagcatc gcgaaggcgt
    71461 ggtgtttttg ttgtggggat cgcatgcgca aaagaaaggg gcgattatag ataagcaacg
    71521 ccatcatgta ctgaaagcac cgcatccgtc gccgctttcg gctcatcgtg gattctttgg
    71581 ctgcaaccat tttgtgctgg caaatcagtg gctggaacaa catggcgaga cgccgattga
    71641 ctggatgcca gtattaccgg cagagagtga gtaaatttgc gggaaaatgc cggatggcag
    71701 agttgccacc cggctgattt atcaggcttt attctgacgc caccattcgc caagaagaac
    71761 gccggttgcg acagagatat tcagcccggc aacgttgcca gtaccatcaa tcttcacgcg
    71821 cagatcgttc ggatcgcgtg cggcatccgg taacccttca tattcctgac ccagcaccag
    71881 taccattttc gctggcagac tggttttgaa cagcggttta ccctgctcgc tggaagttgt
    71941 cactacggtg taacccgcct gacggaaatc atccagcacg ttaacaatgt tgtcgccagt
    72001 aatcggctga acgtgctctg cgccgccttc tgcggtacgg atagccgccc ccgactccag
    72061 cagtgccgca tcctgcacca caacaccttt cacgccaaag tgcgcgcagc tgcgcatcat
    72121 gccacccagg ttatgcgggt tagattcgtt ttccagtgcc agaacacaat cctgcgcgcc
    72181 tgcctggctt acccactgct gcacggttgt gccattacgc tttttgatca ggaagcaaac
    72241 gccaccgtga tgttccgtgc ctgacgcttt tgtcaattcc gcttcatcta ccacatggta
    72301 cgctttgcgg tttgctgcca tccagcgcaa ggcttcttta aaacgcggcg ttacactctg
    72361 gataaaccag gcgcgaacaa tcgcttccgg acggctctgg aacagtgcct gacaggcgtt
    72421 ttcgccgtag acgcgggttt cttccgcacg ctgacgacgc aacacttccg gatcaataaa
    72481 acttttacca ctgatgccac cgtgatcggc cttttccggc gtctcatcac ccggcgcgcg
    72541 ggaaaccgtg cgccacggcg aatcttccca cttgcggtca cggggctgac tctgtttgtc
    72601 atcgcgggcg gggcgacggc caccgtcagc acgagatttt cctggacgcc cgcccccttt
    72661 cccggtacgc gggttgtggg tacgtttatc agaatcatca tcactgcgga catacatcac
    72721 tttgaccttg ccgcttttac ctttcatttc atcgttcatg cttttctcca ccagcgctgc
    72781 gcgaagcgcg cagattaccc gaagtccgcg cggttcgcca tgatttcgta ccaaagcctg
    72841 cgactatcat acctattgaa taaaacagat tgttgtctgg aacaatgtcc ccgataatat
    72901 gtaacatatt agaaacatac cggcgttgtt gccgataagt ctccttactc atcccgaggt
    72961 tagttatgaa taccgtttgt acccattgtc aggccatcaa tcgcattccc gacgatcgga
    73021 tcgaagatgc ggcaaaatgc ggacgctgcg gtcacgactt gtttgacgga gaggtgatta
    73081 atgcgaccgg tgaaacgctc gacaaattgc tgaaagatga tctaccagtg gtgatcgact
    73141 tctgggcacc gtggtgcggc ccctgccgta atttcgcacc aatttttgaa gatgtcgcgc
    73201 aagagcgtag cggtaaagtg cgctttgtga aagtgaatac cgaagctgaa cgtgaattga
    73261 gcagtcgctt tggaattcgt agtataccga cgatcatgat tttcaaaaac ggtcaggttg
    73321 tcgacatgct taatggcgca gtaccgaaag cgccgttcga tagctggctg aacgaatctc
    73381 tttaatctta ccggggcgca tcttgtgccc cgttttctcc tctgcgacaa tggcgttttt
    73441 tcgacgctct cttatgaccg aaaacgctgt tctccagtta cgcgccgagc gtattgcgcg
    73501 cgcaacacgt ccttttcttg cccgcggtaa tcgcgttcgt cgctgccaac gctgtctttt
    73561 gccagagaaa ttatgtctct gttcgacaat taccccagca caagcaaaaa gtcgcttctg
    73621 tttgctgatg ttcgacaccg agccaatgaa gcccagtaat accgggcgtc tgattgctga
    73681 tattttgcct gataccgttg cgtttcaatg gtcgcgtacc gaaccctcgc aggatttgct
    73741 ggatctggta caaaacccgg attatcagcc aatggtggtc tttcccgcct cgtatgctga
    73801 tgagcaacgg gaagtgatct tcacaccacc tgccggtaag ccaccgctgt ttatcatgct
    73861 cgatggtacc tggccggaag ctcgcaagat gtttcgtaaa agtccgtatc tggataatct
    73921 tcccgtcatt tccgtcgatc tttcccggct ttctgcctat cgcctgcgtg aagcccaggc
    73981 tgaaggccaa tattgtactg ccgaggtagc catcgcactg ttagatatgg ccggcgatac
    74041 cggggcggca gcagggttag gcgagcactt cacccgcttt aaaacgcgct atctggcagg
    74101 aaaaacgcaa catctgggta gcatcacagc agaacagtta gaaagcgttt aaaatcattc
    74161 ggtcacttct gcgggagacc ggtatgagtc agcgaggact ggaagcacta ctgcgaccaa
    74221 aatcgatagc ggtaattggc gcgtcgatga aacccaatcg cgcaggttac ctgatgatgc
    74281 gtaacctgct ggcgggaggc tttaacggac cggtactccc ggtgacgcca gcctggaaag
    74341 cggtgttggg tgtgttggcc tggccggata ttgccagctt gccctttaca cccgaccttg
    74401 cggttttatg taccaatgcc agccgtaatc ttgctcttct ggaagagctc ggcgagaaag
    74461 gctgtaaaac ctgcattatt ctttccgccc cggcatcgca acacgaagat ctccgcgcct
    74521 gcgccctgcg ccataacatg cgcctgcttg gaccaaacag tctgggttta ctggctccct
    74581 ggcaaggtct gaatgccagc ttttcgcctg tgccgattaa acgcggcaag ctggcgttta
    74641 tttcacagtc ggctgccgtc tccaacacca tcctcgactg ggcgcaacag cgtgagatgg
    74701 gcttttccta ctttattgcg ctcggcgaca gcctagatat cgacgttgat gaattgcttg
    74761 actatctggc acgcgacagt aaaaccagcg ccatcctgct ctatctcgaa cagttaagcg
    74821 acgcgcgacg ctttgtttcg gcggcccgta gtgcctcgcg taataaaccg attctggtga
    74881 ttaaaagcgg acgtagcccg gcagcacagc gactgctcaa cacgacggca ggaatggatc
    74941 cggcgtggga tgcggcgatt cagcgtgccg gtttgttgcg ggtgcaggat acccacgagc
    75001 tgttttcggc ggtggaaacc ctcagccata tgcgcccgct gcgtggcgac cggttgatga
    75061 ttatcagcaa cggtgctgcg cctgccgcgc tggcgctgga tgccttatgg tcacgcaatg
    75121 gcaagctggc aacgctaagc gaagaaacct gccagaaact gcgcgatgca ctgccaggac
    75181 atgtggcaat ctctaacccg ctcgatctgc gcgatgacgc cagcagcgag cattatgtca
    75241 aaacgctgga cattctgctc cacagccagg attttgatgc gctgatggtt attcattcgc
    75301 ccagcgccgc tgctcccgca acagaaatcg cgcaagtatt aattgaagcg gtaaagcatc
    75361 atccccgcag caagtatgtt tctctgctga cgaactggtg cggcgagcac tcctcgcaag
    75421 aggcacgacg tttattcagc gaagccgggc tgccgaccta ccgtaccccg gaaggaacca
    75481 tcactgcttt tatgcatatg gtggagtacc ggcgtaatca gaagcaacta cgcgaaacgc
    75541 cggcgttgcc cagcaatctg acttccaata ccgcaaaagc gcatcttctg ttgcaacagg
    75601 cgattgccga aggggctacg tcgctcgata cccatgaagt tcagcccatc ctgcaatcgt
    75661 atgggatgaa cacgctccct acctggattg ccagcgatag caccgaagcg gtgcatattg
    75721 ccgaacagat tggttatccg gtggcgctga aattgcgttc gccggatatt ccacataaat
    75781 cggaagttca gggcgtcatg ctgtacctgc gtacagccaa tgaagtccag caagcggcaa
    75841 acgctatttt cgatcgcgta aaaatgacct ggccgcaggc gcgggtccac ggcctgttgg
    75901 tgcaaagtat ggctaaccgt gctggcgctc aggagttgcg ggttgtggtt gagcacgatc
    75961 cggtttttgg gccgttgatc atgctgggtg aaggcggtgt ggagtggcgt cctgaagatc
    76021 aagccgtcgt cgcactgccg ccgctgaata tgaacctggc ccgctatctg gttattcagg
    76081 ggatcaaaag taaaaagatt cgtgcgcgca gtgcgctacg cccattggat gttgcaggct
    76141 tgagccagct tctggtgcag gtttccaact tgattgtcga ttgcccggaa attcagcgtc
    76201 tggatattca tcctttgctg gcttctggca gtgaatttac cgcgctggat gtcactctgg
    76261 atatcgcgcc gtttgaaggc gataacgaga gtcggctggc agtgcgccct tatccgcatc
    76321 agctggaaga atgggtagaa ttgaaaaacg gtgaacgctg cttgttccgc ccgattttgc
    76381 cagaagatga gccacaactt cagcaattca tttcgcgggt caccaaagaa gatctttatt
    76441 accgctactt tagcgagatc aacgaattta cccatgaaga tttagccaac atgacgcaga
    76501 tcgactacga tcgggaaatg gcgtttgtag cggtacgacg aattgatcaa acggaagaga
    76561 tcctcggcgt cacgcgtgcg atctccgacc ctgataacat cgatgccgaa tttgccgtgc
    76621 tggttcgctc ggatctcaaa gggttaggct tgggtcgacg cttaatggaa aagttgatta
    76681 cctatacgcg agatcacgga ctacaacgtc tgaatggtat tacgatgcca aacaatcgtg
    76741 gcatggtggc gctggcccgc aagctcgggt ttaacgttga tatccagctc gaagagggga
    76801 tcgttgggct tacgctaaat cttgcccaac gcgaggaatc atgagtaagg tactggaaat
    76861 gttgaccact taatcgggac tggtgttatt attgcacgct tatgtcgtct gcattgcaca
    76921 gaggaccctt caatgaacag agaagaaatg cactgtgatg ttgtcaaaat ttaagcgtaa
    76981 taaacatcaa caacaccttg cccaactacc caagatttct caatcagttg atgatgtcga
    77041 tttcttttac gctcccgccg acttccggga gacgctgctg gaaaaaatag ccagcgcgaa
    77101 gcagcgcatt tgcattgtcg ccctgtatct cgaacaggat gacggtggca aaggcattct
    77161 gaacgcgttg tatgaagcta aaaggcagcg tccggaactg gatgtgcggg tgctggtcga
    77221 ctggcatcgt gcacaacgtg gacgcattgg cgctgcggca tctaacacta acgctgactg
    77281 gtactgtcgc atggcgcagg aaaatccggg cgtagatgtt ccggtttatg gcgttccaat
    77341 caatacccgt gaagcccttg gtgttctgca ctttaaaggc tttatcatcg acgatagcgt
    77401 actttatagc ggtgccagcc tgaacgatgt ttacctgcat cagcacgata aatatcgcta
    77461 cgaccgttat catcttatcc gtaaccgtaa gatgtcagac attatgtttg aatgggttac
    77521 acagaatatt atgaatggcc gcggcgttaa tcgtctggat gatgttaatc ggccaaaaag
    77581 cccggaaatc aagaacgata ttcgtctgtt ccgccaggag ctgcgtgatg ccgcttatca
    77641 tttccagggc gatgccgaca acgatcagct ttccgtaacg ccgctagtgg ggctggggaa
    77701 atcgagtctg ttgaacaaga ccattttcca tcttatgcct tgtgccgagc agaaactaac
    77761 catctgtacg ccatacttca acctgccagc aatccttgtg cgcaatatta tccagttgct
    77821 gcgcgaaggg aaaaaggtcg aaattattgt tggtgataaa accgcgaacg acttctacat
    77881 tccggaagat gaacctttca agataattgg cgcattgcct tatctctatg agatcaatct
    77941 gcgtcgtttc ctgagccgtt tgcagtatta cgtcaatact gaccagctag tgcttcggtt
    78001 atggaaagat gacgacaaca cctatcacct aaaagggatg tgggttgatg ataagtggat
    78061 gttgatcacc ggtaataacc tgaacccgcg cgcctggcgt ctggatctgg aaaacgccat
    78121 tttgatccac gatccacaac tggagcttgc gccgcagcga gagaaagaac tggagctgat
    78181 ccgcgagcat accaccatcg ttaagcacta tcgcgatctg caaagtattg ccgattatcc
    78241 ggtgaaggtt cgtaaactca tccgccgttt gcgccgtatc cgcatcgacc gattaattag
    78301 ccgcatcctg taatcacaac cccgtcctgt acggggtttg ttttttggag gccacgtttt
    78361 gcgtattctt tttgtctgtt cgctattgtt actttctgga tgcagccata tggctaacga
    78421 tagctggagc gggcaggata aagctcaaca ctttatcgcc tcggcgatgc tttccgccgc
    78481 cggaaatgaa tattcacagc atcaggggat gagccgggat cgcagtgcca tgtttggttt
    78541 gatgttctct gtcagtttgg gggcgtcaaa agagttttgg gatagccgcc ccgaagggag
    78601 cggctggagc tggaaggatt tggcctggga tgtcgccggt gcaagcaccg gctataccgt
    78661 ctggcaactg acccgtcact aaagacgcat ccccttccct ttgcgatgta gcatcaaaga
    78721 aaccagaaac gccaccacgg ccatcaaggt cacataccag aagaaggctg tttccattcc
    78781 tattgatttc agcgacaacg ctacgtactc cgccgaacca ccaaatatag cattagcgac
    78841 cgcatatgac agaccaacgc ctaatgcgcg aacctgtgcc gggaacatct cagccttcag
    78901 tattccactg attgatgtat aaaaactcac tatcagcagg gcacacatca ccagaccaaa
    78961 agcggcataa ggcgaggaaa cgttttgcaa tgctgagaga ataggaacgg taaaaatggc
    79021 tgccagcgaa ccgaaacata acattgaggt acggcgacca atcttatccg acagcgcgcc
    79081 aatgagtggt tgaataagca tgaatacaaa caatgcggca gtcataatgc cactcgccac
    79141 gttggcatgc attcccgcag tatttaccag atacttctgc atataagtgg tgaaggtata
    79201 gaaacaaagg gagcccgcag cggtaaaacc gagaaccatg atgaatgcac ggcgattgcg
    79261 ccataatcct ttcagagatc cagcttcttt taaagcgcgc gtttcttgtt gcgaagtttc
    79321 atctaactga cgacgtaacc acaacgccac aacagctaac acagctccta acgcgaaagg
    79381 aatacgccat ccccactctc tgagtgcaga gtcttccatg gtgtgttgta aaaccacgac
    79441 aaccagtagg gctagcagtt gtccgccgat caacgtcaca tactgaaatg atgcgtaaaa
    79501 acctttgcgc ccttcaacgg caacttcact catataggtg gcgctggtgc catattctcc
    79561 gccaacggat aatccctgaa ataaacgcgc gagaagcaat aatgccggag cccacgtacc
    79621 gattgtttca taaccaggca aacaggcgat taccagtgag ccgaaacaca tcatacacac
    79681 cgatagcagc atcgattttt tgcgaccatg tttatcggct atacggccaa atagccaacc
    79741 gcctattggg cgcatcagga atcccgcagc aaaaacacct gctgtttgta gtagttgagt
    79801 cgtcgtgttc ccggaaggga agaagatgtg ggcaaagtag agtgaacaga acgagtagac
    79861 atagaaatcg aaccactcga ccagattacc tgaagaggcc cccacaatcg cccaaatgcg
    79921 gcgacgagta tcactacttg tcagtttgct gtctgccgtt acagtacttt cagccatgcc
    79981 attatgtctc ctgccgtaat ccgatgcttt tgtcggtcgc ttttgtttat ttcttttggc
    80041 aaagaaaatg ttatacattt gttacgtatc attatgcaac cttaaccatg aatttagtta
    80101 gcaggaaaat ggttattgag gagcttaagg ataaatttct ggtaaggagg acacgtatgg
    80161 aagtaggcaa gttggggaaa ccgtatccgt tgctgaatct ggcatatgtg ggagtataag
    80221 acgcgcagcg tcgcatcagg catttttttc accaacgcaa aaaggccatc cgtcaggatg
    80281 gccttt
//
LOCUS       DAZKIF010000006        69662 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZKIF010000006 DAZKIF010000000
VERSION     DAZKIF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.006"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            143..1207
                     /gene="ulaG"
                     /locus_tag="ACHOTW_000446"
     CDS             143..1207
                     /gene="ulaG"
                     /locus_tag="ACHOTW_000446"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9842621.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1315..2070
                     /gene="ulaR"
                     /locus_tag="ACHOTW_000447"
     CDS             1315..2070
                     /gene="ulaR"
                     /locus_tag="ACHOTW_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP9842622.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2067..2816)
                     /gene="yjfP"
                     /locus_tag="ACHOTW_000448"
     CDS             complement(2067..2816)
                     /gene="yjfP"
                     /locus_tag="ACHOTW_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9842623.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2998..3327
                     /gene="bsmA"
                     /locus_tag="ACHOTW_000449"
     CDS             2998..3327
                     /gene="bsmA"
                     /locus_tag="ACHOTW_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP9842624.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3476..3751
                     /gene="yjfN"
                     /locus_tag="ACHOTW_000450"
     CDS             3476..3751
                     /gene="yjfN"
                     /locus_tag="ACHOTW_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP9842625.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3868..5493)
                     /gene="aidB"
                     /locus_tag="ACHOTW_000451"
     CDS             complement(3868..5493)
                     /gene="aidB"
                     /locus_tag="ACHOTW_000451"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP9842626.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5577..6740)
                     /gene="yjfC"
                     /locus_tag="ACHOTW_000452"
     CDS             complement(5577..6740)
                     /gene="yjfC"
                     /locus_tag="ACHOTW_000452"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9842627.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6743..7381)
                     /gene="yjfM"
                     /locus_tag="ACHOTW_000453"
     CDS             complement(6743..7381)
                     /gene="yjfM"
                     /locus_tag="ACHOTW_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP9842628.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7391..7789)
                     /gene="yjfL"
                     /locus_tag="ACHOTW_000454"
     CDS             complement(7391..7789)
                     /gene="yjfL"
                     /locus_tag="ACHOTW_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP9842629.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7807..8466)
                     /gene="yjfK"
                     /locus_tag="ACHOTW_000455"
     CDS             complement(7807..8466)
                     /gene="yjfK"
                     /locus_tag="ACHOTW_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP9842630.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8517..9215)
                     /gene="yjfJ"
                     /locus_tag="ACHOTW_000456"
     CDS             complement(8517..9215)
                     /gene="yjfJ"
                     /locus_tag="ACHOTW_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP9842631.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9234..9635)
                     /gene="yjfI"
                     /locus_tag="ACHOTW_000457"
     CDS             complement(9234..9635)
                     /gene="yjfI"
                     /locus_tag="ACHOTW_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP9842632.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(9762..10493)
                     /gene="rlmB"
                     /locus_tag="ACHOTW_000458"
     CDS             complement(9762..10493)
                     /gene="rlmB"
                     /locus_tag="ACHOTW_000458"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9842633.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(10674..13115)
                     /gene="rnr"
                     /locus_tag="ACHOTW_000459"
     CDS             complement(10674..13115)
                     /gene="rnr"
                     /locus_tag="ACHOTW_000459"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9842634.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(13154..13579)
                     /gene="nsrR"
                     /locus_tag="ACHOTW_000460"
     CDS             complement(13154..13579)
                     /gene="nsrR"
                     /locus_tag="ACHOTW_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP9842635.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(13784..15082)
                     /gene="purA"
                     /locus_tag="ACHOTW_000461"
     CDS             complement(13784..15082)
                     /gene="purA"
                     /locus_tag="ACHOTW_000461"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9842636.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(15186..15383)
                     /gene="yjeT"
                     /locus_tag="ACHOTW_000462"
     CDS             complement(15186..15383)
                     /gene="yjeT"
                     /locus_tag="ACHOTW_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP9842637.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(15465..16469)
                     /gene="hflC"
                     /locus_tag="ACHOTW_000463"
     CDS             complement(15465..16469)
                     /gene="hflC"
                     /locus_tag="ACHOTW_000463"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP9842638.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(16472..17731)
                     /gene="hflK"
                     /locus_tag="ACHOTW_000464"
     CDS             complement(16472..17731)
                     /gene="hflK"
                     /locus_tag="ACHOTW_000464"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP9842639.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(17817..19097)
                     /gene="hflX"
                     /locus_tag="ACHOTW_000465"
     CDS             complement(17817..19097)
                     /gene="hflX"
                     /locus_tag="ACHOTW_000465"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP9842640.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(19174..19482)
                     /gene="hfq"
                     /locus_tag="ACHOTW_000466"
     CDS             complement(19174..19482)
                     /gene="hfq"
                     /locus_tag="ACHOTW_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP9842641.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(19568..20518)
                     /gene="miaA"
                     /locus_tag="ACHOTW_000467"
     CDS             complement(19568..20518)
                     /gene="miaA"
                     /locus_tag="ACHOTW_000467"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9842642.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(20511..22358)
                     /gene="mutL"
                     /locus_tag="ACHOTW_000468"
     CDS             complement(20511..22358)
                     /gene="mutL"
                     /locus_tag="ACHOTW_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9842643.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SENAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(22368..23705)
                     /gene="amiB"
                     /locus_tag="ACHOTW_000469"
     CDS             complement(22368..23705)
                     /gene="amiB"
                     /locus_tag="ACHOTW_000469"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP9842644.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(23724..24185)
                     /gene="tsaE"
                     /locus_tag="ACHOTW_000470"
     CDS             complement(23724..24185)
                     /gene="tsaE"
                     /locus_tag="ACHOTW_000470"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9842645.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(24157..25704)
                     /gene="nnr"
                     /locus_tag="ACHOTW_000471"
     CDS             complement(24157..25704)
                     /gene="nnr"
                     /locus_tag="ACHOTW_000471"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP9842646.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            25703..26842
                     /gene="queG"
                     /locus_tag="ACHOTW_000472"
     CDS             25703..26842
                     /gene="queG"
                     /locus_tag="ACHOTW_000472"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9842647.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(26825..26878)
                     /locus_tag="ACHOTW_000473"
     CDS             complement(26825..26878)
                     /locus_tag="ACHOTW_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9842648.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(27112..27187)
                     /locus_tag="ACHOTW_000474"
     tRNA            complement(27112..27187)
                     /locus_tag="ACHOTW_000474"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27152..27154),aa:Gly,seq:gcc)
     gene            complement(27224..27299)
                     /locus_tag="ACHOTW_000475"
     tRNA            complement(27224..27299)
                     /locus_tag="ACHOTW_000475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27264..27266),aa:Gly,seq:gcc)
     gene            complement(27510..28055)
                     /gene="orn"
                     /locus_tag="ACHOTW_000476"
     CDS             complement(27510..28055)
                     /gene="orn"
                     /locus_tag="ACHOTW_000476"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP9842649.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            28150..29202
                     /gene="rsgA"
                     /locus_tag="ACHOTW_000477"
     CDS             28150..29202
                     /gene="rsgA"
                     /locus_tag="ACHOTW_000477"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP9842650.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            29299..30267
                     /gene="asd"
                     /locus_tag="ACHOTW_000478"
     CDS             29299..30267
                     /gene="asd"
                     /locus_tag="ACHOTW_000478"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP9842651.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            30289..33612
                     /gene="mscM"
                     /locus_tag="ACHOTW_000479"
     CDS             30289..33612
                     /gene="mscM"
                     /locus_tag="ACHOTW_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP9842652.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(33763..35265)
                     /gene="yjeM"
                     /locus_tag="ACHOTW_000480"
     CDS             complement(33763..35265)
                     /gene="yjeM"
                     /locus_tag="ACHOTW_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP9842653.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(35484..36461)
                     /gene="epmA"
                     /locus_tag="ACHOTW_000481"
     CDS             complement(35484..36461)
                     /gene="epmA"
                     /locus_tag="ACHOTW_000481"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP9842654.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            36786..38594
                     /gene="frdA"
                     /locus_tag="ACHOTW_000482"
     CDS             36786..38594
                     /gene="frdA"
                     /locus_tag="ACHOTW_000482"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP9842655.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            38587..39321
                     /gene="frdB"
                     /locus_tag="ACHOTW_000483"
     CDS             38587..39321
                     /gene="frdB"
                     /locus_tag="ACHOTW_000483"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP9842656.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            39332..39727
                     /gene="frdC"
                     /locus_tag="ACHOTW_000484"
     CDS             39332..39727
                     /gene="frdC"
                     /locus_tag="ACHOTW_000484"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP9842657.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            39738..40097
                     /gene="frdD"
                     /locus_tag="ACHOTW_000485"
     CDS             39738..40097
                     /gene="frdD"
                     /locus_tag="ACHOTW_000485"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP9842658.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            40160..41293
                     /gene="ampC"
                     /locus_tag="ACHOTW_000486"
     CDS             40160..41293
                     /gene="ampC"
                     /locus_tag="ACHOTW_000486"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9842659.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            41381..41914
                     /gene="blc"
                     /locus_tag="ACHOTW_000487"
     CDS             41381..41914
                     /gene="blc"
                     /locus_tag="ACHOTW_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9842660.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(41911..42228)
                     /gene="sugE"
                     /locus_tag="ACHOTW_000488"
     CDS             complement(41911..42228)
                     /gene="sugE"
                     /locus_tag="ACHOTW_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9842661.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(42404..42550)
                     /gene="ecnB"
                     /locus_tag="ACHOTW_000489"
     CDS             complement(42404..42550)
                     /gene="ecnB"
                     /locus_tag="ACHOTW_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9842662.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(42661..42786)
                     /gene="ecnA"
                     /locus_tag="ACHOTW_000490"
     CDS             complement(42661..42786)
                     /gene="ecnA"
                     /locus_tag="ACHOTW_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9842663.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(42838..43404)
                     /gene="efp"
                     /locus_tag="ACHOTW_000491"
     CDS             complement(42838..43404)
                     /gene="efp"
                     /locus_tag="ACHOTW_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9842664.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            43446..44474
                     /gene="epmB"
                     /locus_tag="ACHOTW_000492"
     CDS             43446..44474
                     /gene="epmB"
                     /locus_tag="ACHOTW_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP9842665.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            44709..45578
                     /gene="yjeJ"
                     /locus_tag="ACHOTW_000493"
     CDS             44709..45578
                     /gene="yjeJ"
                     /locus_tag="ACHOTW_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP9842666.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(45771..46124)
                     /gene="yjeI"
                     /locus_tag="ACHOTW_000494"
     CDS             complement(45771..46124)
                     /gene="yjeI"
                     /locus_tag="ACHOTW_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP9842667.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(46262..47908)
                     /gene="groL"
                     /locus_tag="ACHOTW_000495"
     CDS             complement(46262..47908)
                     /gene="groL"
                     /locus_tag="ACHOTW_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9842668.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(47952..48245)
                     /gene="groS"
                     /locus_tag="ACHOTW_000496"
     CDS             complement(47952..48245)
                     /gene="groS"
                     /locus_tag="ACHOTW_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9842669.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            48521..49777
                     /gene="yjeH"
                     /locus_tag="ACHOTW_000497"
     CDS             48521..49777
                     /gene="yjeH"
                     /locus_tag="ACHOTW_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP9842670.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(49793..50269)
                     /gene="fxsA"
                     /locus_tag="ACHOTW_000498"
     CDS             complement(49793..50269)
                     /gene="fxsA"
                     /locus_tag="ACHOTW_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP9842671.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            50606..52042
                     /gene="aspA"
                     /locus_tag="ACHOTW_000499"
     CDS             50606..52042
                     /gene="aspA"
                     /locus_tag="ACHOTW_000499"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP9842672.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            52160..53461
                     /gene="dcuA"
                     /locus_tag="ACHOTW_000500"
     CDS             52160..53461
                     /gene="dcuA"
                     /locus_tag="ACHOTW_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP9842673.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            53577..53915
                     /gene="cutA"
                     /locus_tag="ACHOTW_000501"
     CDS             53577..53915
                     /gene="cutA"
                     /locus_tag="ACHOTW_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP9842674.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            53891..55588
                     /gene="dsbD"
                     /locus_tag="ACHOTW_000502"
     CDS             53891..55588
                     /gene="dsbD"
                     /locus_tag="ACHOTW_000502"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP9842675.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLTIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            55625..56200
                     /gene="yjdC"
                     /locus_tag="ACHOTW_000503"
     CDS             55625..56200
                     /gene="yjdC"
                     /locus_tag="ACHOTW_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9842676.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            56307..56382
                     /locus_tag="ACHOTW_000504"
     tRNA            56307..56382
                     /locus_tag="ACHOTW_000504"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56340..56342,aa:Phe,seq:gaa)
     gene            56580..56739
                     /locus_tag="ACHOTW_000505"
                     /pseudo
     CDS             56580..56739
                     /locus_tag="ACHOTW_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            56999..58537
                     /gene="cadC"
                     /locus_tag="ACHOTW_000506"
     CDS             56999..58537
                     /gene="cadC"
                     /locus_tag="ACHOTW_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFP9842677.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            58903..60237
                     /gene="cadB"
                     /locus_tag="ACHOTW_000507"
     CDS             58903..60237
                     /gene="cadB"
                     /locus_tag="ACHOTW_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFP9842678.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            60317..62464
                     /gene="cadA"
                     /locus_tag="ACHOTW_000508"
     CDS             60317..62464
                     /gene="cadA"
                     /locus_tag="ACHOTW_000508"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFP9842679.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            62523..63980
                     /gene="dtpC"
                     /locus_tag="ACHOTW_000509"
     CDS             62523..63980
                     /gene="dtpC"
                     /locus_tag="ACHOTW_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFP9842680.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            64217..65734
                     /gene="lysS"
                     /locus_tag="ACHOTW_000510"
     CDS             64217..65734
                     /gene="lysS"
                     /locus_tag="ACHOTW_000510"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9842681.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(65853..66026)
                     /gene="ghoT"
                     /locus_tag="ACHOTW_000511"
     CDS             complement(65853..66026)
                     /gene="ghoT"
                     /locus_tag="ACHOTW_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP9842682.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(66054..66350)
                     /gene="ghoS"
                     /locus_tag="ACHOTW_000512"
     CDS             complement(66054..66350)
                     /gene="ghoS"
                     /locus_tag="ACHOTW_000512"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9842683.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            complement(66577..66849)
                     /gene="yjdJ"
                     /locus_tag="ACHOTW_000513"
     CDS             complement(66577..66849)
                     /gene="yjdJ"
                     /locus_tag="ACHOTW_000513"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9842684.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            complement(66861..67091)
                     /gene="yjdI"
                     /locus_tag="ACHOTW_000514"
     CDS             complement(66861..67091)
                     /gene="yjdI"
                     /locus_tag="ACHOTW_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP9842685.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            67272..68903
                     /gene="dcuS"
                     /locus_tag="ACHOTW_000515"
     CDS             67272..68903
                     /gene="dcuS"
                     /locus_tag="ACHOTW_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9842686.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            68900..69619
                     /gene="dcuR"
                     /locus_tag="ACHOTW_000516"
     CDS             68900..69619
                     /gene="dcuR"
                     /locus_tag="ACHOTW_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP9842687.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
BASE COUNT        17115 a        18240 c        17154 g        17153 t
ORIGIN      
        1 tctttccata ctcaaaatca acaacaactt ccctggcaaa aagtcaaaaa atggtcattg
       61 ccttccagaa aaaatagtgt caggattaat caaaaccaat cacatattga ttcgaattga
      121 actatcatga aggtaaatgg cgatgagtaa agtgaaaagt atcacccgtg aatcctggat
      181 cctgagcact ttcccggagt ggggtagctg gttgaatgaa gaaattgaac aagaacaggt
      241 cgctcctggc acatttgcga tgtggtggct tggctgcacc gggatctggt tgaaatcgga
      301 aggtggcacc aacgtttgcg ttgatttctg gtgcggcact ggcaaacaaa gtcacggtaa
      361 cccgttaatg aaacagggtc accagatgca gcgcatggct ggcgtgaaaa aactgcagcc
      421 aaacctgcgt accaccccgt ttgttcttga tccgtttgcg attcgccaga tcgacgcggt
      481 actggcgact cacgatcaca acgatcatat cgacgttaac gtcgctgctg ccgtgatgca
      541 gaactgtgct gatgacgtac cgtttatcgg accgaaaacc tgtgtggatt tgtggattgg
      601 ctggggcgta ccgaaagagc gttgcatcgt ggtcaaaccg ggcgatgtag taaaagtgaa
      661 agacattgaa attcatgcgc ttgatgcttt cgaccgtact gcactgatca ccctgcctgc
      721 cgatcaaaaa gcggctggcg tactgccaga tggcatggac gatcgcgcgg tgaactacct
      781 gttcaaaacg cctggcggct ccctgtatca cagcggcgac tcccactact ctaactatta
      841 tgcgaagcac ggtaacgaac atcagatcga cgtggcgtta ggatcgtacg gcgaaaaccc
      901 gcgcggtatc accgacaaga tgaccagcgc cgatatgctg cgtatgggtg aagcgctgaa
      961 tgcgaaagtg gttatcccgt tccaccacga tatctggtca aacttccagg ccgatccgca
     1021 agagatccgc gtgctgtggg agatgaaaaa agatcgtctg aagtatggct tcaagccgtt
     1081 tatctggcag gtgggcggca aatttacctg gccgctggat aaagacaact tcgagtacca
     1141 ctatccgcgc ggtttcgatg attgcttcac tattgaaccg gatctgccgt tcaagtcatt
     1201 cctgtaatct gtcgtttata ccggatagcg tggagaagtt cgcgctatcc ggcgtagtat
     1261 ttcctggcta tttcaaatat catctaaaaa aatcaaattt tatcggaata gctcatgact
     1321 gaagcacaaa gacatcaaat cctcctggaa atgctcgcac aattgggctt tgtgaccgtt
     1381 gagaaagtcg ttgagcgtct gggaatttcg cctgccactg cgcgacgcga tatcaataaa
     1441 cttgacgaaa gcggcaaact gaaaaaagtg cgcaatggtg cagaagctat tacccaacag
     1501 cgcccgcgct ggacgccgat gaatctgcat caggcgcaga atcacgatga aaaagtacgt
     1561 atcgctaaag cggcctcgca gctggttaat ccgggcgaaa gcgtagtcat caactgcggc
     1621 tccaccgcgt ttctgcttgg gcgggaaatg tgtggcaagc cagtgcaaat catcactaat
     1681 tatctaccgc tggcaaatta cctgatcgat caagaacatg acagcgtgat cattatgggc
     1741 ggacagtaca acaaaagtca gtccatcact ttaagcccgc agggcagcga aaacagtctc
     1801 tatgccgggc actggatgtt taccagcgga aaagggctga ccgcagaagg gttgtataaa
     1861 accgatatgc tgacagcaat ggcagagcag aagatgctga gcgtggtagg gaaactggtg
     1921 gtactggttg atagcagtaa gattggcgaa cgcgcgggaa tgctttttag ccgtgccgat
     1981 caaatcgata tgcttatcac cggcaaaaat gctaacccgg aaatcctgca acaactggaa
     2041 gcgcaagggg tcagcattct gcgtgtttaa agatgctggc ggaaaaatgt cacggcagca
     2101 tctaacgcct caggcgtaat gcggtgacgc acgcctggct gccatgaaca ggttagcagt
     2161 ttatcccgtc ccgtctcgct taaggcctgc tgcaaacgta gtgattcgtc ggcaggcaca
     2221 acgtcatcgt cgaggccata ccacagcagt agaggtctgt cactaagttg ttccaggtgg
     2281 tttgtcgctt cccactctgc cagtggcgcg acaatgttat tgaattcatt ctgctgtgct
     2341 accgtttcag gtatcagcgg tggaaacagt gaacgggcga gtgatgtaaa atagcccgat
     2401 cccatcatgc tggcggtaca tctcaccgtg gggtggcgag cggtaatccc cagtgccgtc
     2461 atcgcgccca tcgacgcacc accgactgcc agacggtcat caagcagcca cttttcttcg
     2521 gctattgccg cacgtaaagt agtgaattcc tgcatacttt gtagcaagat ttgccagaat
     2581 tgatttaacc gccgcgctgc gtcaccacta aaacggctac cgtgatcggg cgcatccggc
     2641 atgatcaccc gcaaaccagc ctgcgccagc gcaacggcaa aatagctata caccagactg
     2701 gacgaagtaa agccgtgata aaaaatcacg cacggtaacg gggtatcttt ttgtccgaca
     2761 ggataagcat gaagaacggg aatatctgcc agctcgcgtg attctatttc aatcatctgt
     2821 ctctcctttt tgtctggtat gacatgaaga aatccttagc acaagtttct gctggcggta
     2881 ttagttcata atgttgagat gtgggttacg ctttcgtcac gttttcattc gaaaatcgcg
     2941 taccaggtaa caattcggga acattccccc aaagttaaat taataaggca ctacactatg
     3001 gttagcagga aacgtaatag cgttatttac cggtttgcca gtttattatt ggtgttgatg
     3061 ttaagtgcct gtagcgcact gcaaggtacg ccacagccag caccgccagt aacggaccat
     3121 ccgcaagaga ttcgccgcga ccagacgcaa gggttacagc gaataggtag cgtaagcaca
     3181 atggttcggg gttctccgga tgacgcatta gcagaaatca gagcaaaagc tgtcgctgca
     3241 aaagctgatt attacgttgt cgtcatggtg gacgaaacca tcgtgacagg acagtggtat
     3301 tcacaagcca ttttgtaccg taaataaatc agacaactta catagtcagc tttacattgc
     3361 tttgcgtccc tatgcgttta cctgtgcaca ataaattacc ggctggaagc ctgggcgttt
     3421 attaccgcga tggaatgccc tgcaacatgt ggggaaacga aaaatggagc tgacgatgaa
     3481 acaattactt gcctcaccct cgctgcaatt agtgacttat cctgcgagcg ccacggcaca
     3541 gtctgcagaa ttcgccagtg ctgactgcgt gacgggcctg aatgaaatag gtcagatctc
     3601 agttagcaat atttcagggg atccgcagga cgtggaacgt atcgtggcgt taaaggccga
     3661 tgaacaaggt gcgtcatggt accgcattat taccatgtac gaagaccagc agcctgataa
     3721 ctggcgcgta caggcgatac tgtatgcatg agtgtcatct acagcaaatg aacaatgccg
     3781 gatgcggcgc gagcgccttg tccggcctac aattttcaca tatttcattt agttaatcga
     3841 aaccagcgtc gcatcagtcg tatacgctta cacacacact ccccccgtcg cccgcagcaa
     3901 taaatcattc tggatctgct ctgacaaccg tacgccgccg cgcgtatcta acatcacctg
     3961 acaccacgcc tgcgccattg gcggagaagc atatttcaac atttgcgcac cgcagcccag
     4021 caggaatagc tgatgagtaa tctctcgccc cagttcttca gctggcttac gcagctgctg
     4081 ctgtaaacga cgaaccgcgc aatcaaaata gcgatcctgc cctttcactt ctacaaatgc
     4141 ttccgacaat aagtcgtata cgcccgcttg cttattgaga acgcgcagca catccaggca
     4201 cataatattg ccggaacctt cccaaatact gtttaccggc atctcccggt aaagccgcgg
     4261 cagctcgctc tcctcgcaat aaccaatgcc gcccagcacc tccatcgctt cggccacaaa
     4321 cggcatacct cgtttgcaga tcacaaattt tgccgcaggc gtaaataaac gcgcccacag
     4381 agcttctttg gcatcggcgc gccggtccca cgctcgcgca agacgaaaca gcaacgccgt
     4441 ttgcccttca agctgaagtg ccatgcgact taagacatga cgcataaggg gctgttggat
     4501 caatggatta ccaaaaacat ggcgttgatg cgcatgataa atcgccagcg agaacgcacg
     4561 gcgcatcatg gcatggctac ccagggcgca atcaaaacgc gtcatccccc ccattttcag
     4621 gatcagacga attccttccc cttcctgccc caataaccag ccaatggcat cctgaaactc
     4681 cacttcacaa ctggcgttag agcgattacc cagcttatct ttcagccgct cgaggcgaat
     4741 cgcgttgcgt tgcccgtcag gcaaaaagcg cggcacaaaa aagcaggaca aaccgcccgc
     4801 ggtctgcgcc agcaccagat gcgcatcgct ttgcggaact gagaaaaacc atttatgccc
     4861 caccagccga taagagccat cctccagacg ctctgcgcgg gtggtgttgc tcatgacatc
     4921 ggaaccgccc tgcttttccg tcattcccat gccaatcaac aaaccgcgtt tttgcccacc
     4981 tggcaataag tgagaatcgt agcgatcgct caacagcggt gtggtccagt cctgaaacgg
     5041 cgcgggtaac atctgtaaca acaatggcgt ggcggcaaag gtcatggtta tcggacataa
     5101 cgaccccgcc tcaacctgtg cgtgcaacat aaaacgcgcc gcgcgcgcca caaatgcgcc
     5161 ggagcgagcg tcttcttccc aggcaagatt gtgcacccga ttggtacata gtgcctgcat
     5221 cagcaggtgc caggcggggt gaaaacgcac atcgtccaga cggcgtcctt gcgcatcgta
     5281 gcgcaacagt tcaggcggat tcacattcgc cagccgcccc agttcaaggg attcagccgt
     5341 tcctaactgc tgaccaatac tggctagaaa atcgctatcc cagccagcac cttcacgcgt
     5401 taccgcttcg caaagcgcgc catcagacag gtataagttg ctgttattta atggtatagg
     5461 ttgattaaaa acggtgtgag tttgccagtg cactgtgtct ccctccatca atggcagtca
     5521 ccattagtat ggtcactgcc atggattcat gacagaaaga gattgctaaa acattcttat
     5581 ccagcaatgt aatgcggaac gaaacgtgag gtgtctttgg tgatcagtgt gttatcttca
     5641 cggatcccca ttccgcacgc ttcatcatcg acaatccagc taccgatgag tgtgtagcta
     5701 tcgccaaacc gcggcagagg ttgaaacgcc tggtagatca tcggttcatc ggcgtaatca
     5761 ccatcagcgt ggtcaacgac gttattctgg ccgtcgaaaa tggtgacatt tccgccttcg
     5821 cgcgagtaga tcggtttacg cacatagctt tcgccagcgg caatctgcgg tttttcaccc
     5881 tcaaaccagg atgcaagaag gttaggatga ccagggaaga agcgccaaag cagcggcatt
     5941 agccctttat tactcaagat acttttccat aacggctcca cccattgctc gcgacgcttg
     6001 cgcagcagcg gaccgttatc gtcacgcatc atccactcca gcggatacag cttaaatgca
     6061 cgctggatga cattatcatc aagatcggtc agtacgccgc cgacgcccaa accgagatct
     6121 tcaatgtaga taaaccgcga ctcctgccct gcctgctggg cgcagtcctg caagtacagc
     6181 acggtactcc tgtcttcatc ggtgtcctga cagcagcaaa aataaaacgg ttcccgactg
     6241 taaagctcgc taaagcgtga aatcaggcgt tcctgaatag cattgtactg atcggcatca
     6301 cgcggaataa tgccgctgcg ccgggcatcc tccagccaca gccactggaa ataagccgac
     6361 tcgtacaatg aagttggcgt atcggcgttg tactccagca gcttcaccgg cgcattgcca
     6421 caccaggcaa aatccatccg tccatacagc gaaggatcgc gggcgcgcca gctttcagcg
     6481 atcacatccc agtacaacgg cgggatcgcc agttgcgtca ggatctcttc atcttttacc
     6541 gcgcgatcca ccacctcaag gcacatctga tgcagttccg cagtcggttt ttcgatctgc
     6601 tcttcaatct ggcgcagagt aaaacggtaa gcccgactct catcccaata gatttcattg
     6661 tcgatgatat gaaagtcgaa accgttgtca gcggcgatct ggtccagatc ccgtcgcaca
     6721 ggaacgttgt gtctcagcat gattagcctc cccaatgccc acgggcgctg gaagaacgac
     6781 catagccgcc gcgagaaacg gttgacgctt ttttggtggt aaagccgcct gaagagtaag
     6841 actctttttt gccagaaccg gagcgccagg agtaatcacc ggaagtgttg cgccagacgg
     6901 ggcgggaagc aaatgaggat ccgccgctgt tatagacaaa cggctcatcg cgatctttac
     6961 gaataacccg gcttaacaaa aatccagaaa cgaccgggat ccaactctgt tcaacattgt
     7021 cgtagtagca attttcgtac ttagactgac agttctgctg agtcatattc ttcggaacat
     7081 cggcataaaa tgccgctttg gcgttgttcc agccacgcgc gcaaatgtcg gcattattac
     7141 cgtcatcaat acaatcctgc actgtcgaat aaaacgttcc gtcgccgtcg ttatcgacat
     7201 cgccactatc gccacaacct ttcaatacaa aaaaagcggc accgcccatt agcgccaacg
     7261 ttaagtattt ttgtgcgtag cgattgcgac gcggttcaaa aggattattc ggtctaccaa
     7321 tgcgactgat cgctccgtga ccaattttac tgttatttct tgatttgcgt tttctggcca
     7381 tgatcgtcca ttaccatgtc atacaagctg cgttaaaaat accgccagcc agcgcggcgg
     7441 ttcccataaa cattcctgct gcggtattgt gattaataat tttttcgctt aatgcgggca
     7501 tataaagtct gacaccagca aaaacgagta actgaatcac cagtgcaatc ccgccccagg
     7561 cgaaatagtc tggaatactc accgcattga ttgccgcact ggataagggg atcacgtaac
     7621 ccaacaatgt accgctgaac gccagtgacg ctgcggtatt attgtttttg attaattgcc
     7681 attcattgtg cggtgtaatt ttggagtaga taaacagaaa aataatcacc atagccacgc
     7741 caataaaaaa ataggcacta aaggcaagaa gtgaatccag tatatgcatt gtaaacgtcc
     7801 ttgtaattaa ccaataatat gaagtgatgt cagcgggata tcgacaccca acgcgcgcga
     7861 aaataaccac tctggctcgc cgagatcgtt aaaagattcc tcaccattta gcagcaaata
     7921 ttcgtaagta tcttcggtca cttgccgctg gtagcccatg gtaaaattat ggacttccca
     7981 tttagcatga ttttgatttt ctactttttc cagcatgtag acgggttcga tatttcccgg
     8041 ttcttcgctg ttaaaaaatc gctgccagcg tttttcctgc cagtttaagg tcattgcccc
     8101 catcgttttg gcgttgatcg cctcgcgcca gtgattctct ttactgatac cgtagctctc
     8161 ttcatagaca aagagcttga tatcatcaat gtcatcaata tcttcgccgc cggtggtatt
     8221 gatttgcaga aactcatcgc ctgaagtgta gtagcggaaa atctgactac cgccgcccag
     8281 atcgatgtgg ctgacagcga ctaccgtaaa ttcctcaccc ggcagcgcaa tcagcaattc
     8341 atcttccagc aaacgaaacg ctaacgtgtc gagcgtaaag ccactattga gatgaagtcc
     8401 cagcggaccg cgagcgatag caggcttatt atccttgcca aacagacgct ggaaaaaacc
     8461 agacatattt tatccttaaa atagttgcca gccttgcagt gaggctggca aaaaaattac
     8521 tcgccctgtt ggcgttgcag tcttgctaat acatcctggg cgctactttt attgctaccg
     8581 ccaattccgg cttccgccag cttttcgtca aggtcgcgac cgtctgcgac tttttctaac
     8641 tgtgcggcag catccaggcg agcctgacgt tcggcctgac gcgtttgcag gcgttttaag
     8701 gattctgctg ctgtcgaaac gctggaagat gcgccaacgg tagaagtcgt tacagcctgt
     8761 tgtgcacgct gcatggcttc agtggcttta acgacttcca tttgctgctc aaactgagcg
     8821 atacgctgcg ctgtcgctgc aactgctttt tccacgccat cacgggagac ttccagattc
     8881 gacaacactt gctcttcagc ggtaatgaga ttctcaaggc gcgcgatttc ttcagcaact
     8941 tcgttaatca acgacggatt aacgttcttg cttaacgctt ccagcgtacg ggcttccaga
     9001 ctggctttgc gctcacgtaa atctttcagc ttatcgtgac ttaatttcac ccgcgccagc
     9061 agatcaacgc gagattttcc ggctttatcg agttcagctt tagcgtcacg aatatgctgt
     9121 tccagcatgc gcacaccctg ggtttcttca atggattctt ccgcctggga gataaacgat
     9181 ttccccagcg taaataaact ttttaaaatt cccataaata cccgtccttg taattagtgt
     9241 gaatattctt ctgtaatttc agccagatcc aatgcgttat ctaccagcga ggttatctca
     9301 agcaggatat cttcaagaga agatttaagc gataacgccc cgaaaacaat gtaatactct
     9361 tcttgttgta cgctggagat tcctaccgat gacaacggca tcatcttctg atttcttaat
     9421 aagaaggtat taaattcatc tggattgctg atactactca ccggacaaat aaaggtttcg
     9481 ataatcattt ggcgggaagt aaaaagaata ttgatttgca aatcgccata atcattcatt
     9541 ttaataatta atgcgttctc gctatttgtt acatcaatat tttgttcagg tacagtttgc
     9601 agcgccgtcg ccagcgccaa cggattccat gtcatatatt attccatata gattaagttt
     9661 aaatattaat aaaatgaata tttgcaatac gtaattatct taccagctat agacaaaaaa
     9721 aaaccatcca aatctggatg gcttttcata attcagagga actagctgcg ctgacgaacc
     9781 gcttcaaata agcaaattcc ggttgcaacc gaaacgttca gggaagaaac acttcctgcc
     9841 attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat accttcacct
     9901 tccgcgccca tcaccagcgc caggcgtccg gtcattttgc tttgatagag cgtatgatcc
     9961 gcctcacctg ccgtaccgac gatccagata ttctcttcct gcaacatacg catggtgcgc
    10021 gcaaggttag tcacccgaat cagtggaacg ctttctgccg cgccgcaggc tacttttttc
    10081 gccgtggcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcgtg aacgccagca
    10141 gcgtccgcgc tacgcaggca cgcgccgagg ttgtgcggat cggttacacc gtcgaggatc
    10201 agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc ctggtactga
    10261 cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct tttctcgtcg
    10321 agatattggc ggtttgccaa ctggataacc acgccctggg actcaagggc gtggatcagc
    10381 ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg
    10441 gcgcgctcca gcagggcctg cactgcatgg atgccgtaaa tcatttcgct cattaatgta
    10501 ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gtctacatga tccctgcaat
    10561 atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
    10621 caaagcgcat tttgtcagca atctaaccct cttctgtttg aagagggtaa tgatcactct
    10681 gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cagctatttt ctgcgttttc
    10741 gccgatggct ttttcgcttt tctcgcgtct ttcttcgctg ctttcggctt cgtttttttc
    10801 tcaccgcgga aggcgctgtc tggctcaaag tttacctttt taccgacctg acgacgcttg
    10861 ccgccttttt tacctgcatc gccttttttc gctttctcgc gcgccgtttt accgacgttg
    10921 cgcggtgcgc gttcgctgga gatcaggcta aagtcgattt tgcgctcgtc catattaacc
    10981 gcttcgacgc gaacttccac gcgatcgccc aggcgataag tctggccgct ggattcaccc
    11041 atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga agagacatgg
    11101 accagcccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc agtgacgctg
    11161 gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca cttcagccag
    11221 tcagacacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca gtgctgaccc
    11281 aattgcagca tctcttccat cgaataatgg tagccaccgg tttcagtggt gttgccctga
    11341 tgcccctgct ctttcgccag cagatactta atggcgcggt gcagcatcag gtcaggataa
    11401 cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccaggccaaa gtgaccacgg
    11461 ttttccggat cgtaaatcgc ctgtttcatc gagcgcagca gcatggtttg cagcatttct
    11521 gcatcaggac ggtcggcaac cgactccagc agctccgcgt agtcgcgcgg ttccggcttg
    11581 ttaccgcccg gcagttccag ccccagctct gccagcactg aacggaaaga ggtaatcgct
    11641 tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt ctcaacgaaa
    11701 cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaacttgtg tgcgtcgtta
    11761 cgctgagtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa cttcgcttct
    11821 tcgctctcaa atgagatccc gccgcgttct tcacgggctt tatccagcac tttatagagg
    11881 ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag atcctgatcg
    11941 ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct catcaccgct
    12001 tcgtagaatt tgtagccagt caggcggcct ttcgacgaaa ccgtcatctc gcacaccata
    12061 cacaggcggt ctacctgcgg attgagcgag cacagaccgt tagagagcac ttccggcagc
    12121 atcgggataa cctgcgaagg gaagtacacc gacgtaccac ggttacgcgc ttctctgtcc
    12181 agcggcgttg gcggacgcac atagtagctg acgtcagcaa tcgcgaccca taaacgccag
    12241 ccgccgccgc gttttttctc gcagtaaacc gcatcgtcaa agtcacgggc gtcttcgcca
    12301 tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc ccgcttttgc ttcttccggc
    12361 acttcttctt tcagcccagc aacctgttgc tcaacagcct gcggccagat gtacggaatt
    12421 tcatgggtac gcagagcgat atcaaccgcc atgcccgtgc ccatattgtc gcccagcact
    12481 tcgacgattt tacccaccgc tttggtacgg cgagtcgggc gttgggtcag ttcaacaacc
    12541 accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat atcgaagctc
    12601 agacggctat cgtcaggaac cacaaagccg acgcccgctt cggtaaagta gcgaccaaca
    12661 atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg acctttacgg
    12721 tcagcaccca gcggctgagc cagcacctga tcgccatgaa tgcaggtttt catctgctcg
    12781 ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta gccatcacgg
    12841 tggccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata gcactgacga
    12901 cgagtaaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg caggccttca
    12961 agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct ggccggtttt
    13021 tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt cgcgtatttt
    13081 tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt gtcatctctg
    13141 atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc tcttcaacca
    13201 aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc ttagaaagcg
    13261 cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa ttcaccagcg
    13321 ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc ggtttaccca
    13381 ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga ctaagttgat
    13441 tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact tcagaaatac
    13501 tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat ccgtaatcag
    13561 tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat acatttatat
    13621 tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg aaggaaaaaa
    13681 gcgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc aatgtatcac
    13741 actgttagcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg tcgaacgggt
    13801 cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg atcggtacac
    13861 cggtcagctc ttcaatgcgc ttgatgtagt tcagcgccgc ctgcggcagg ccgctacgat
    13921 ctttcacgcc gaaggtggat tcagaccagc ccggcatggt ttcgtaaatc ggctctacac
    13981 ctttccagtc gtcagctgcc agcggagtgg tagtcacttc acgaccatcc ggcatacggt
    14041 aagccacaca gagtttcacc tctttcaggc catccagaac gtccagttta gtcaggcaga
    14101 agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc agccagccgg
    14161 tacgacgacg acgacccgta gttgcgccga attcgttacc ctgcttgcag aggaactcgc
    14221 cagtttcatc aaacagttca gtcgggaacg gacctgcacc cacacgagtg gagtaagctt
    14281 tgaggatacc cagaacgtaa tcaacataac gcgggcccag gccggaaccg gtcgccacgc
    14341 caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg atatccagca
    14401 gcgtaccctg cgcaccttcg aacatgacga aatcgccacg ctgacgcgcc tggtcgagca
    14461 ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc gtatcatcca
    14521 gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg aagttgtgat
    14581 attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg tcgccaacac
    14641 gcagaccgcg acgtgccact ttatcttcat aagcaggccc gataccacga ccggtggtgc
    14701 cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagcgcaaca tgataatcaa
    14761 ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg ccacggtctt
    14821 ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg ttaccgatga
    14881 tgctagttac attctcgcgg agaatacctg atggaataag atggagaacg gttttttcac
    14941 cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca acatatttag
    15001 cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat tgggtgccca
    15061 gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat ggattctacc
    15121 atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat cagtctgttt
    15181 tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc aaccacaagt
    15241 ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga gatcatcttc
    15301 ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac cagggcaagc
    15361 gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc accgacatcc
    15421 ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg cgttgcggaa
    15481 gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat cacgtcctga
    15541 ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta gaagtccgga
    15601 tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc accgcgcatg
    15661 atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc ggcagtcgcg
    15721 cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac cgcttcacgc
    15781 tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt gatctgctta
    15841 attcgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt gatgaccgga
    15901 actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc gttatctgcc
    15961 gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc gtcacgtact
    16021 tcgagggtca gacgaccacg ggaatcggtg acgatgtctt tcacgtccag gcgaccaatt
    16081 tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg cgaaatgtcg
    16141 cccccccccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt gatgtaagag
    16201 tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt gtccatggtc
    16261 tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa atgcagaccc
    16321 ggctcataaa ccagaggttt gttgtcatcg tcacgcagta ccttaccaaa acgcagcgta
    16381 ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac cagcacgatg
    16441 atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg tagtcgttac
    16501 gctgcgcatt ggcgcggcgt tggtccataa tatcgccctg actggtggac gacgtgttgc
    16561 ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg ctggcaccgt
    16621 tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac ggcagaacca
    16681 tcaggttgcc acctttatcg ttaaccagca ctttgcgggt gttacccaac actttttcca
    16741 tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat tccggcagaa
    16801 gtttagcaaa acgcgccact tcaccctgag cttccaggat ggtctgggcc ttgtacgcac
    16861 gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact tcgttggtat
    16921 acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc gcatcgtcaa
    16981 acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc agcagcgtga
    17041 tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg ctacgaatca
    17101 cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact ccacgcaggg
    17161 cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac agatattttt
    17221 ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc tcgtccgacg
    17281 tcagcatcac accagaagct gccagttcac gcacggcttc cacgttcacc ggtttgactt
    17341 cgtcgataaa cgtcggtttc cagttcagac ctggctcaac cagatggctg aatttaccaa
    17401 agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg gccgcccaga
    17461 taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc gggcgcgggc
    17521 cttgcgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga ccaccgagct
    17581 ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg cgaccgcctt
    17641 tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg tcgcggtctt
    17701 gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt tatgcggtga
    17761 tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct acgccgttag
    17821 atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc aacgatcggc
    17881 atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc tattgcctga
    17941 agctgataaa aacgacttct cagacgccct tcctgcggtg gcagacgcaa tgtatgctgc
    18001 gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa gctgtggtat ccccgctccg
    18061 gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc aatacgcggt
    18121 tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat ctcgtgagcg
    18181 tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg cacatccgcc
    18241 gcatcaatca catgcagcag caatgtggct tgccgcgtct cttgtaacgt ggctttaaat
    18301 gctgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc aagtacggtt
    18361 tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc aaacaactgg
    18421 tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt accggcgttg
    18481 gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg ccgcccctgc
    18541 tcacgctgct tttcaactct ttccaggcgc gactgtatct gcacgatgcg attacgcaac
    18601 aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaaccaat cccgcctttc
    18661 tgtctttcaa ggtgggtcca gccacgcacc aggcgtgtag ccagatggcg cagctgcgcc
    18721 agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat atctaaaata
    18781 aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg ctcttgcgcc
    18841 gggctcaggg catggtcaaa aagaacgacc gaagcacccg tcgctttgac agcttccgca
    18901 atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt acggctaccg
    18961 gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa ctcctggagg
    19021 tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg ctcaccagca
    19081 tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac gcaggatcgc
    19141 tggctccccg tgtaaaaaaa acagcccgaa accttattcg gtttcttcgc tgtcctgttg
    19201 cgcggaagta ttctgcgcgc tgctaccatg atggtagtta ctgctggtac cgccaccggc
    19261 gttgttactg tgatgagaaa ccgggcgaga cgggacaaca gtagaaatcg cgtgcttgta
    19321 aaccatctgg ctgaccgtgt ttttcaacag gatcacgaac tgatcaaaag actcgatttg
    19381 cccttgcagc ttaataccat tcaccaaata aatagaaact ggaacacgtt cccgacgcag
    19441 tgcgttcagg aacggatctt gtaaagattg ccccttagcc attctctctt ttccttatat
    19501 gcttatttgt actttgaacc tttcgattct gaaaaaattg cgcacgatac gtctcaattg
    19561 tacacattca gcctgcgata gcaccaacaa cctgtaatac ttcgtcacgc gcctgttctg
    19621 gtttttcact gtcaagccag tgaacccctt cccaaccacg cagccaggtt atctgccgct
    19681 tcgccaactg tctcgtggcg caaacacctc gataaaccat ttcatcgtat gagatttcgc
    19741 ctccaaggta agaccacatc tggcgataac ccacgcaacg aatggaaggc aagtccgtat
    19801 gcaaatctcc tcgggcaaaa agcgcccgga cttctgcttc aaaacctgaa gccaacatct
    19861 gatgaaaacg ctgctcaatt cgttgatgga gcagttcacg gctcgccggg gcgatggcga
    19921 actgatgcac ctgatacggt agagcgtctc ctgacgtttg cgtcagttcc gttaaagttt
    19981 tacccgaaat gaaaaaaact tccagtgccc gggaaagcct ttgtggatca tttggatgaa
    20041 tccttgctgc cgcaaccgga tcgatctcct gaagttgacg atgcaatgac tcccaacctt
    20101 gctctgccgc ctgttgctca attctggccc gtacttccgg gtctgccgac ggtagcggcg
    20161 acaacccttc cagcaatgcc ttgaaataca acatcgtacc gcccactaac agtgggatcc
    20221 gccccgccgc ggtgatatcg gccatttccg ccagcgcatc gcggcgaaaa tcagcagccg
    20281 agtaagcctg cgacggatcg cgaatatcca gcaatcggtg cggcgcggcg agtaactctt
    20341 cagcgttcgg cttcgccgtc ccgatatcca tccctttgta aataagggca gaatcaacgc
    20401 ttatcaactc tactggtaaa attttacgca gctcaatggc taacgccgtt ttaccggagg
    20461 ccgtcggccc catcaaaaaa atcgccttag gcaggctcgc cttactgata tcactcatct
    20521 ttcagggctt ttatcgccgg atgtaaatca acagattgta acagaccacc cggcggcgtt
    20581 ttcacaagtt gcggacataa ccgttccacg tccgccagca gggttatggc ctgtgccatt
    20641 gaccactgcg cattttcgct catcagattt cgtgcaatcc actgcgcaat attgccaggt
    20701 tcgaatacgg actgcttcgc taggtagcct atcagttcag gaatcaagat ttgtaaattt
    20761 tgttggcgta agggtaaagg cacggccctg atggtcacat gctgtgcatc tgactggaaa
    20821 tcaataccca attccgccag ggcagactgc gctttttcta atgccgattt ttcttcgcca
    20881 gaaactttta gccgcaacgg aatcagcaac ggctgggcgc aaacgggcgc ttcacccggc
    20941 gtcagttgta cctgacgcag ccaacgttct gccactggca aggctaaaag tgaaatgttg
    21001 ccgtcgcgct ccagcaacgc acagtcggaa tggacgatag tcagtacccg accaaaactc
    21061 tgactgttcg ccgcaagtgc aggttcctgc ggttccggcg cttttggttt ttgcatcggc
    21121 gcgggcgttt gcaaaagctg gcgatacact tcaccttgct gtttctggta acctggctgc
    21181 gcattcggcc agggggcagc cggacgacta cctgatgctg gcgcaggagt gtagcgcgga
    21241 gctactggct cacgaactgc cggttcagca aagtgattgc gccccgccgc cacgcggttt
    21301 tccggaatgg gacgcggtgc aggttggggt tcatcgtcca gcggtagcgg cgtttccagt
    21361 tgctgttgta gcacgctcag cacgccctga tagataaagt catgcaccag acgcgactga
    21421 tgaaaacgca cttcgtgttt ggcggggtgc acgttgacgt ccacctgatg cgggtcgatc
    21481 tccagataca acacaaatgc aggttgctga tcggccccca gtttgtcttc acaggcctgg
    21541 cggatcgcgt gattgatcag gcgatcgcgc atcatgcgac cattcacgta gcaatactga
    21601 atttccgcca gtgcgggcgt ggtgtgattt ggatcggcca cccagccgcg cagcgtgaga
    21661 tcgccgtgtt gccattcaat cgccagcgct tgttcgagaa aagcggtgcc acaaatcgcg
    21721 cctaagcgcc gttctttttg cccgccttcc ggcaccgcgc ggtactggcg tacgattttg
    21781 ccgttatgcg acaggttgat cgtgacgtcg aaacgcgcca gtgcgatgcg gcggatgatt
    21841 tcatcgatat ggttaaattc ggttttctcg gtgcgcagga atttgcgccg cgccggggtg
    21901 ttgtagaaca ggtccagtac ctccagcgtc gttcccaccg gatgcgccgc cggtttaacc
    21961 gtcacgtcca tatcgcgccc ttcggcatag gcctgccagg cttcctgctg ttctgcggtg
    22021 cgtgaagtga gcgtcaggcg ggaaaccgaa ctgatactcg ccagcgcctc accgcgaaag
    22081 cccaggctga taatggcttc gagatcgtcc agagaggcga ttttactggt ggcatgacgc
    22141 gccagcgcca gcgccagctc gtcttttttg ataccacagc cgttatcacg aatgcggata
    22201 agtttcgccc caccgcgttc gatatcaata tcgatacgcg tcgcacctgc atcgaggctg
    22261 ttttccacca gttctttgac taccgacgca ggtcgctcga ccacctcacc tgcggcaatc
    22321 tggttcgcca gttgtggcgg taagacctga attggcatca atcgtcctta gtttggcagc
    22381 gtgcgatctg gcgtcgtcac cgtactggca gtttgtgccg ttgcaccctg cggcgcagat
    22441 tgcatcggat gcgcaaggaa ataattgcgc aggcctttgt aaatggcttc tgccagctgt
    22501 tgttggtaat cgtcgctcgc cagcaaacgt tcttcgctgt tgttgctgat aaaaccggtt
    22561 tcgaccagta ctgatgggat atccggcgag cgcagaacgc caaggctggc gtgttctggt
    22621 cgacgtttat gtatttcgcc aatgcgttgc aactgactga tcatactggt cgctacatca
    22681 taccctaccc gctgggaatg accgaactgt aaatccagca ccgcctggct taaatagggg
    22741 tcagactgac tgttcgccag cacatcaccc gccccgccca gcagctccga ctgtttctcg
    22801 tgctgctcca gccagctggc catttcgctg ttggcgcgac ggttagagag cacccatacg
    22861 gaagcgccag tcgcactgcg gttcggtgcg gcatcagcgt gaatcgacac gaggaaattg
    22921 gcgttttgct tacgtgccac atcgctgcgc cccatcaccg agataaagta atccccgtca
    22981 cgggttaaaa cgcctttaaa catcggatcg tcattgagca aagtacgcaa tttacgcgcg
    23041 atggcgatgg tgacattttt ctcccgcgta ccaccggggc cgatagcgcc agggtcctga
    23101 ccgccgtgtc cggcatcaat agcgatgata attttatcgc cattgttagc cgtcgcgcgc
    23161 gctgccggac gcgttaccgt attactgctg ataacacccg tagtgcggtt actttccgtt
    23221 ttaaacggat tgcgcgccgg ttcgctgacg cgcggtgcga caaccgtagg cgtttcaacg
    23281 cgtttcacaa ccacaggcgg cggaggaggc ggtggcggcg catcggcgtt aatcgtaaag
    23341 acgacagtgt aattgctgcc attctgccgc ttcaccgctt cggttttacc attttcggta
    23401 agatcgacca ccagccgtag cgtttgtgca tctttaggcg ttccggagcg aatcgccttc
    23461 accagattat taccgctgaa caacaacggc agtccctgaa tcacgcccgt ttgtttgata
    23521 tcgagcgcca cggtgcgttt gctttgatgg ctaaacgcat aatcaggatc gccaataaaa
    23581 ctcaacgtta tccgcgcctg ttggttgccg ttagaaacct gaatatcaga gagcgtcgcg
    23641 gcacccacct gcgcgcacag cagcagcaac gtcgctacca accaatttct gatgcgatac
    23701 atcatcccac cacctttcaa aggttaaccg gctaaacgtg ccagcaacaa ttcacccgca
    23761 gaggaaaccg cactcacgcg cgcctcacgg ccttgtgcct gataatcaat gtgtatttcg
    23821 acatccgggt caggaagaac acctgtacct tgttgtggcc actccaccag gcagatggca
    23881 tcgttggcaa aataatcgcg gatccccata aactccagct cctcgggatc ggcaaggcgg
    23941 tacaaatcaa agtgataaac cattaaattg tcgagcgtat agggttcgac cagcgtataa
    24001 gtggggcttt tgacattacc ctgatggccc aaagcctgta aaaagccccg gctaaaggtg
    24061 gttttacctg cgcctaaatc gccatacaga tagattacgg ttgcgccatc gcaggctttc
    24121 gctacccgct cgcccaggtc taatgttgcc tgctcatcag ggagcggaat tactcgattc
    24181 atcatggttt ttatcagtca cttccgggtt aacaatacgc tgtagcgtgg aaaagagatc
    24241 ggttgccagc atcccgcgcg ttccaaaacg cgccgccagt acgtcagctg ccgcaccgtg
    24301 cgcgacacag cctgcacagg ctgcatcata cggcgacagt ttttgcccaa gcaatgcgcc
    24361 aataatacca gagagcacat cgcccatgcc gccgcttgcc atgcctgcat ttccggcatc
    24421 aataatgcct aaagcgtcag gatgggcggc gaccacggtt ccggcacctt tcagcaccgc
    24481 tacgccgcca taacgttgta ccagacgttt ggcgcaatgt aagcggtcac tttcaatttc
    24541 agcgacggaa cagcctaaca accgtgcggc ctcgccagga tgcggcgtga tcacgcgatt
    24601 gtgacgctta tcgggattga ttgccagcag gttcaatgca tcggcatccc acaacatcgg
    24661 tttgcgaaaa ttctcaactt tttgcagggc ttttttcccc cactcttgct ggcccagacc
    24721 gggaccaatc accaccacat cggcccattc caggctttcg gtaagagagt ccatagtcag
    24781 ttcatgcacc atcaattctg gtcgtgcagt cagcagcggc gcaatgttct cactgcgggt
    24841 cagtactcgg actaaaccag caccagcacg tagcgccgct tcccccgtca tacgaatagc
    24901 ccccgccgtg ccgtgatcgc caccgataat caccagccgc ccgtgatcgc ctttatgcga
    24961 agtcgggcga cgcggtttca gccagtgaga aagttgttct gccgaaaacc gctgaatttt
    25021 cgtctcctga cctgccagcc agctatccag ccccagtgag tcaaaatgca gttgtcctgt
    25081 aacatcccgc gcttttccag tgagcaagcc tggtttcagc gcaataaaag tgatggtgtg
    25141 atcggcgttg atcactgcgc ctggcgtagc gccggtttca gccagcaggc cggaagggat
    25201 atcaaccgcc acaatcggcg caggatggga attagcgtgg tcgattaact ggctaatgga
    25261 ttcgcggggt gcttgctgca agccggtacc gagcagcgca tcaacaatca gagctaccga
    25321 ctcgggccag acaatattcg aagcatggat ctcgcctccc gcgtttaacc atgcctcgcg
    25381 tgccagcgcg gcctcttccg gcaacggttt atcgctctcc tgggccaaca acgtgacctc
    25441 aatgccgacc gctttggcca gtcgcgcgac cacgtagcca tcgccgccgt tattaccatg
    25501 accgcacaac accagccagt ggcgggcgtc aggatacgcc gaacgacaca cctggaatgc
    25561 ggcctcgcca gcgcgaagca tcagctcata gagtgtgagc cccagcgcat ctgccgcctc
    25621 gcgttctccg cggcggatat cgtcggcgtg ccagacggtg tgtggtatac ttacggggtt
    25681 tttcttcatt gtatggtccg tcatgtcaga gcccctcgat ctcaatcagt tagcgcaaaa
    25741 aattaaacag tgggggctgg aactgggctt tcagcaggta ggtattaccg ataccgatct
    25801 cagcgagtcc gagcccaaac tgcaagcatg gctggacaaa caataccacg gcgaaatgga
    25861 ctggatggca cgtcacggta tgctgcgcgc tcgcccccat gagttattgc ccggtacgct
    25921 gcgcgtgatc agcgtgcgga tgaattacct tcctgctaac gccgcatttg ccagcacgct
    25981 gaaaaacccc aaactcggct atgttagccg ttatgcgctg ggccgtgact atcacaaact
    26041 tctgcgcaac cgactcaaaa agctgggcga gatgattcag caacattgtg tttcgctgaa
    26101 ttttagaccg tttgtcgatt ctgcgcctat tctcgagcgc ccgttagctg aaaaagctgg
    26161 gctcggctgg acaggtaagc actcacttat cctcaatcgc gaggccggtt cgttcttctt
    26221 tttaggcgaa ttgctggtcg atattccgct gcccgtggat caaccagtcg aggaaggatg
    26281 cggcaaatgc gtggcctgta tgacgatttg cccgaccggt gccatcgtcg agccatatac
    26341 cgtcgatgct cgccgctgta tctcttatct caccatcgaa ctggaagggg cgatcccgga
    26401 agagttgcga ccgttaatgg gaaaccgtat ttacggttgc gatgactgcc agcttatctg
    26461 cccgtggaat cgctattcac aactcactac agaagacgat ttcagcccgc gtaagccgct
    26521 acacgcaccg gaactcattg agttattcgc ctggagcgaa gagaagtttt taaaagtcac
    26581 ggaaggttcg gcgattcgcc gtataggtca cctgcgctgg ctgcgtaata tcgccgtggc
    26641 attaggcaat gcgccctggg atgaaacgat tctagcggcg ctcgagagtc gcaaaggtga
    26701 gcacccactt cttgatgagc acatagcgtg ggcgatggcg cagcaaatcg agaggcgaaa
    26761 tgcgtgcata gtcgaagtgc aattgccgaa aaaacagcgt ctggttcggg taattgaaaa
    26821 agggttaccg cgtgacgcct gattcatcca cagcttgtga ataaaaataa aaacgcattg
    26881 caattcaaga cgaataaaat cgtcaagtga tcgactcgac aaaacgaaat cttaattttt
    26941 aaattatcta tcaataggtt aaaaaaatac tattcaccgt gcgaagtttc tttgaaatta
    27001 agattacggc ctgaggctgt ggataactct gtttacaaga gtttgtcaga gacaacaaaa
    27061 accagcaata acgcatcgcg tcgctgtgga tattttattg agagaagaat ttggagcggg
    27121 aaacgagact cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta
    27181 ttcccgcttg ggtggtctgt gctttgcagc acttttcaaa ttttggagcg ggaaacgaga
    27241 ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc tattcccgcg
    27301 tactacttaa ttttgctttc gtaattttcg catttctgcg tcgttacggg aggcgcatta
    27361 tacgagaaat ctttccctct gcaacccccc taaaaatttt tttttgagtt tttatctcaa
    27421 gtgctgaaat tatcagcaag acgttcaatt tagcgtcaaa atcacgccgc cgcaagtgga
    27481 ttccggcgct tacgtgacca gcaaaaattt tacagcttga taaaatgctc gcggtagtaa
    27541 gccagctccg ccaccgattc acggatatca tccatcgcct gatgcgtccc ctgtttggta
    27601 aaaccatcaa gaatttccgg cttccagcgg cgcgccagct ctttcagggt gctgacatcg
    27661 agataacggt agtggaagta ggcttccagc tccggcatgt atttaaacag gaagcgacga
    27721 tcctgaccga tgctgttacc gcaaatcggc gatttacccg caggcaccca ctgttttaaa
    27781 aattcgagcg ttgccagttc agcttcacga tcgcccatcg tgctcgcttt cacgcgctcc
    27841 accagcccgc tggcggtatg ggtgcgcacg ttccagccat ccatcagcgc cagctgttca
    27901 tcggactgat gtactgcaat ggtcggccct tctgccagaa tattcagatt ggcatcggtc
    27961 accagcgtgg caatctcaat aatgcgatcg cgctcgggat ccagaccggt catctcaaga
    28021 tcgatccaaa tcaggttgtt ttcattggca ctcatgctat tttccaccca ttcacagtga
    28081 ctatattgat ccacaattgc gtgtatcata gatgttttgc ccatcagggg cgaccaggag
    28141 tcagtacgat tgagtaaaaa taaactctcc aaaggccagc agcgccgcgt gaacgccaat
    28201 caccagcgtc gtcttaaaac gtctaaggag aagcccgact acgacgacaa tctgtttggc
    28261 gagcctgatg aaggtatcgt catcagccgc tttggtatgc acgctgatgt ggaatccgcc
    28321 gatggcgacg ttcaccgctg caatattcgc cgtaccatcc gttcgctggt gaccggcgac
    28381 cgcgtagtct ggcgtccggg taaaccggcg gcggaaggcg taaatgtcaa agggatcgtg
    28441 gaagcggtgc atgagcgcac ctcagtgttg acgcgtccgg atttctacga cggcgtgaaa
    28501 cctattgccg ccaacatcga ccagattgtc attgtctccg ccattttgcc ggagctgtcg
    28561 ctcaatatta tcgaccgtta cctggtggcc tgcgaaacct tgcagattga gccgattatt
    28621 gtgctcaaca agatagacct gctggacgac gaaggcatgg cgttcgtcaa cgaacagatg
    28681 gatatctacc gcaatatcgg ttatcgcgta ttgatggttt ccagccatac tcaggatggg
    28741 ctaaaaccgc tggaagaggc gttgaccggg cgcatcagca tttttgccgg gcagtctggc
    28801 gtcggcaaat ccagcctgct gaacgcgttg ctggggctgc aaaaagagat cctgaccaac
    28861 gatgtctctg acaactcggg gcttggtcag cacaccacga ccgccgcacg cctgtatcac
    28921 ttcccgcacg gcggtgatgt gattgattcc cctggtgtgc gtgagttcgg cctctggcac
    28981 ctggagccgg aacaaatcac tcagggcttt gtcgaattcc atgactattt aggtctgtgt
    29041 aaatatcgcg attgcaaaca cgataccgat ccgggctgcg ctatccggga agcggttgag
    29101 gaaggaaaaa tcgcggaaac ccgtttcgaa aactatcacc gtattctgga aagcatggcg
    29161 caggtaaaaa cgcgtaaaaa cttttctgat acggatgact gacaattaag ctaaccgtcg
    29221 ttagaatcgt cccctttttt tcaggatccg gcatttaggc cggatcagga acgacaaaac
    29281 aatggcctgg aggctacctt gttaaattca tttaaacttt cgctacagta cattctgccg
    29341 aaactatggc ttactcgcct ggcgggttgg ggcgcaagca agcgggcagg atggctgaca
    29401 aaactggtta tcgatctgtt cgttaaatac tacaaggtcg acatgaaaga ggcgcaaaag
    29461 ccggacaccg ccagctaccg cacctttaac gaattctttg tccgtccgct gcgtgacgaa
    29521 gtacgcccaa tcgataccga tccgaatgtc ctggtcatgc ctgccgatgg cgttatcagc
    29581 cagttgggta aaatcgaaga agataaaatc ctgcaagcca aaggccacaa ctacagcctc
    29641 gaagccctgc tggcaggcaa ctatctgatg gcggacctgt tccgcaacgg tacgtttgtg
    29701 accacttacc tctccccgcg tgactaccac cgcgtacaca tgccgtgcaa cggtattctg
    29761 cgtgagatga tctacgtgcc gggcgatctc ttctccgtta accatctcac ggctcagaac
    29821 gtgccgaatc tgtttgcccg taacgaacgc gtgatctgcc tgttcgatac cgaatttggc
    29881 ccaatggcgc agattctggt cggggcgacg attgtaggca gcattgagac ggtctgggcg
    29941 ggcaccatta cgccgccgcg cgaaggcatc atcaagcgct ggacctggcc tgccggggaa
    30001 aacgacggtt ctgtggcgct gctgaaaggc caggaaatgg gtcgctttaa acttggctct
    30061 accgttatca acctgtttgc accgggtaaa gtgaatctgg ttgagcaact ggaaagcctg
    30121 tctgtcacga aaattggcca gccgctggca gtatctaccg aaacctttgt tacgccagac
    30181 gctgaacccg ccccgcttcc tgctgaagag atcgaagcag aacacgacgc cagcccattg
    30241 gttgacgata aaaaagacca ggtctaatcc atcaaaggaa acgctgacgt gcgcctgatt
    30301 atcacttttc tgatggcctg gtgcctcagt tggggggcgt acgccgcgac ggcccccgat
    30361 agcaaacaaa tcacccagga actggagcag gcaaaagcgg cgaaacccgc gcagccagaa
    30421 gtcgtagagg cactccagtc tgccttaaat gcgcttgagg aacgaaaagg ttcccttgag
    30481 cgcatcaagc aatatcaaga agtcattgat aattatccga aactctccgc tactctgcgc
    30541 gcacaattaa acaacatgcg tgacgagccg cgcagcgtgt cgccggggat gtctaccgac
    30601 gcgctgaatc aggaaattct ccaggtcagc agtcagttgc tggataaaag ccgtcaggcc
    30661 cagcaagagc aggagcgcgc ccgcgagatt gccgattcgc tgaatcaact gccgcaacag
    30721 caaaccgacg cccgccgtca gttaaatgag atcgagcgcc gcctggggac gcttaccggc
    30781 aatactccgc tcaatcaggc acaaaatttc gcgttgcagt ctgactctgc acgtcttaag
    30841 gcgctcgttg atgaactgga gctggcgcag ctgtctgcca ataaccgcca ggaattagcg
    30901 cgcttgcgct ctgagctggc tgaaaaagag agccagcaac tggatgcgta tttgcaggcc
    30961 ttgcgtaatc aattgaacag ccaacgccag cttgaggcag agcgggcgct ggaaagtacc
    31021 gaattactag cagaaaacag tgccgatttg ccgaaagata tcgtcgcgca attcaaaatt
    31081 aaccgcgaac tatcggcggc gctgaatcaa caggcgcagc ggatggatct cgttgcctcg
    31141 caacagcgtc aggctgccag ccagacgtta caggtccggc aggcgttgaa tacgctgcgt
    31201 gaacagtcgc aatggctggg atcgtccaat ctgctcggtg aagcgttgcg ggcgcaggtg
    31261 gcacggctgc cggaaatgcc gaaaccacaa cagcttgata ccgaaatggc gcagttgcgt
    31321 gtgcaacggt tacgttatga ggatctgctt aataaacagc cgctgctacg gcaaattcat
    31381 caggccgacg gtcagccgct gaccgctgag caaaaccgta ttctggaagc acagctacgc
    31441 actcagcgtg agttgctgaa ctcattgttg cagggtggcg atacgctact gctggaactg
    31501 accaagctga aagtctccaa cgggcaactg gaagatgcgc tgaaagaagt gaacgaagcg
    31561 acccaccgct atctgttctg gacctctgac gtgcgcccga tgaccatcgc ctggccgctg
    31621 gaaatcgccc aggatctgcg tcgtctcatt tcgctggaca ccttcagtca gttgggcaaa
    31681 gccagtgtga tgatgctgac cagcaaagag acgattttgc cgctgtttgg cgcgttgatt
    31741 ctggtcggtt gcagtattta ctcgcgccgc tatttcaccc gttttcttga acgttcggcg
    31801 gcgaaagtcg gcaaagtgac tcaggatcac ttctggctga cgttgcgcac tcttttctgg
    31861 tcgattctcg tcgcgtcacc gctgccggtg ctgtggatga cgctgggtta cggcttgcgc
    31921 gaggcgtggc cttatccgct ggcggtcgcg attggtgatg gcgtcacagc caccgtgccg
    31981 ctgctgtggg tagtgatgat ttgcgctacc tttgcccgcc cgaacggctt gtttatcgct
    32041 cattttggct ggccgcgcga acgtgtttct cgtgggatgc gctactacct gatgagcatc
    32101 gggcttattg tgccactgat tatggcgctg atgatgttcg ataacctcga cgaccgcgaa
    32161 ttttccggtt cgctgggacg gctttgcttt atcctcattt gcggtgcgct ggcggtggtc
    32221 accctcagcc tgaaaaaggc cgggatcccg ctgtatctca acaaagaagg cagcggcgac
    32281 aatattacca accatatgtt gtggaacatg atgattggcg cgccactggt tgccattctg
    32341 gcgtcggcgg tgggttatct ggcaacggca caggcgctgt tagcgaggct tgaaacctcg
    32401 gttgccatct ggttcctgct actggtggtt tatcacgtta tccgccgctg gatgctgatc
    32461 caacgacgca ggctggcgtt tgaccgggcg aagcatcgcc gggcagagat gttagcgcaa
    32521 cgtgcgcgtg gcgaagagga agcacatcat cacagtagcc cggaaggggc aattgaagtc
    32581 gatgaaagcg aagtcgatct cgatgccatc agtgcgcagt ccttgcggct agtgcgctca
    32641 attttgatgt tgatcgccct gctttcggtc attgtgctgt ggtcagaaat ccattccgct
    32701 ttcggcttcc tcgaaaatat ttcgctgtgg gatgtcacct ccacggtaca gggcgtagaa
    32761 agtctggagc cgattaccct cggtgcggtg ctgattgcca ttctggtgtt tatcatcacc
    32821 acgcagctgg tgcgcaatct gcccgcgctg ctggaactgg cgattttgca gcacctggat
    32881 ttaacgccgg gtaccggcta cgccatcacc accatcacca aatatctgct gatgctgatt
    32941 ggcgggctgg tcggcttctc gatgattggt attgagtggt cgaaattgca gtggctggtc
    33001 gccgcgctcg gtgttggtct cggttttggt ttgcaggaaa ttttcgccaa ctttatctct
    33061 ggcctgatca tcctgttcga aaaaccgatt cgcattggcg atacggtgac aattcgcgat
    33121 cttactggta gcgtgacgaa aattaatacc cgcgcaacca ccatcagcga ctgggaccgt
    33181 aaagagataa tcgtgccgaa caaggcgttt attaccgagc agtttatcaa ctggtcgctc
    33241 tctgactcgg tcacgcgcgt ggtgttgacg attccggccc ctgccgatgc caacagtgaa
    33301 gaagtgacgg aaatcctgct caccgcagcg cgtcgctgct cgctggtgat cgacaacccg
    33361 gcaccggaag tcttcctggt ggatctgcaa caggggattc agattttcga gctgcgtatt
    33421 tacgccgctg agatgggtca ccgtatgccg ctacgccatg agatccacca gctgattctg
    33481 gctggcttcc atgcccacgg tatcgatatg ccattcccgc ccttccagat gcgtctggaa
    33541 agcctcaacg gtaaacaaac ggggagaacg ctgacgtctg cgggcaaagg tcgtcaggcg
    33601 ggaagtttgt aatgcatgaa gctgccaaat gatgtcaacg tggctcattt agcagcttaa
    33661 catcatcttc agttatcacc ggccctggag agtatcatcc atacgcatta cttcctgaca
    33721 actcatcgcc ggagagacga tgagttgcag gatttcatat gatcagtcga gagctaactc
    33781 aggtttattc gccatgcgac tgcaatagtt ctggtaaatc gccatcgcta acagcgagaa
    33841 gaagacaggg ccgccaatca tccacaatgt gctgtcccag tctccggctt caaccacagg
    33901 ctgaataatg gtgaagacgt tcgcaaatgt caccaccaga acgacaacca ccgtcgcaat
    33961 cattgccgac atacgcgttt tgaaaatcac aaatggtctg tcgagatcct gacgtgcttt
    34021 aaagaatggg aaagccagcg cgaggaacag gtaaggaagc gtcatagaca cgttcgccat
    34081 cagcgtcagc ttgttaaaga acgccgatgc ggtaccgcca ccaaacgaaa ccagcaggat
    34141 gaagacagta accaacccgc actgcatcca catagcgata gacggcatcc ccatcgcatt
    34201 caggcgcgtc atcggttccg gccacaacgc tttcggcgtc ccctggatga tggctttcag
    34261 cggtgaatag caaagcgtaa agaacgcacc ggtataagca aggaacatcg aaagtccggt
    34321 aatacgcgca aaccatacgc ccagcgacaa tgacgcttca ggtgacaaat gcagtgcgtt
    34381 acccagcgtc accccgaggc tcttcatcag cacataggta atattgccga ggttaacgga
    34441 accattactt aatacctgct gccagtttgt gctgacgccc cataaaaata ttgccagcga
    34501 ataaccgatt gaaataacaa tagcggcaaa aacaatacct ttggcaaagt tcttttctgg
    34561 attttccgtt ttatcgacca gaccaccaac cgcttcaatt ccgccatagg caaaaatagc
    34621 aaataccaca aacgatagca ttgccagacc ggactgataa cctgggttcg gtgatgcaag
    34681 gaaattaata tcctgcgcga aatgcccacc atttaataac aaaatagtaa tgcttactaa
    34741 cagcaatact aaattcagac acattactgc aataccgccc accgcagtaa tgcgggcaat
    34801 tttattaatc ccctttgaag caacgacggt gaccagaatc atccatgcca ctgccagcag
    34861 accaaccacc tgcgtaggct ccagtccggc aatacgccag tgctgggtca tgtcgctacc
    34921 atagaggaat gttgagaacg gtacccaaac tttcgcggag gtgctcacca tccagatgat
    34981 ataagaggaa aaccacataa acgtaccaat gaaggcaaaa cgtggtccga cactattatt
    35041 catccaggaa tagataccgc cttcttcttt gcgataagca gctcccattt cagccatcat
    35101 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
    35161 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
    35221 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
    35281 ttcagcgcgt gtataaaacg cgcccactga actgcactgc ccaacattga gcgggcagcg
    35341 taaaaaccaa tacagaattg atacctggat gatatcaaat atccagtctt aaattgtggc
    35401 ctttagcgga aaaatgaaaa catatgctac agaatggcgc ttatcacgcc attcttcgct
    35461 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
    35521 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
    35581 cagtcaggca taccgacttt caaggcttca atcagattct gatcaatggg gtgctgcggc
    35641 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
    35701 tccgtcaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
    35761 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
    35821 tggtacacaa aggtcggttt ttctttacca atatttggtt ctacgccaaa ggtaaacagc
    35881 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
    35941 gctgccactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
    36001 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
    36061 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactcaagc
    36121 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaaact gcggcacagc
    36181 tggaataccg gcccgcaacc ggcaaccagc aggcgtttca tatggtattc cgggctggtc
    36241 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
    36301 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
    36361 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
    36421 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
    36481 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaataaa
    36541 acaacggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
    36601 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
    36661 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
    36721 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
    36781 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
    36841 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
    36901 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
    36961 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
    37021 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
    37081 tgtgggggtg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
    37141 gcatgaaaat cgagcgtacc tggttcgccg ccgataagac cggcttccat atgctgcaca
    37201 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
    37261 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaatgaac atgatggaag
    37321 gcacgctggt gcagatccgt gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg
    37381 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
    37441 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcatccaacc ggtctgccag
    37501 gttccggtat cctgatgacc gaaggttgcc gcggtgaagg cggtattctg gtcaacaaaa
    37561 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
    37621 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
    37681 ggcgtaaagg caacaccatc tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
    37741 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
    37801 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgaccgca cactacacca
    37861 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
    37921 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
    37981 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
    38041 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
    38101 atctggttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
    38161 ctatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
    38221 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacact tccagcgtgt
    38281 tcaacaccga cctgctctac accattgaac tgggccacgg tctgaacgtt gctgaatgta
    38341 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
    38401 gttgcaccaa gcgtgacgac gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg
    38461 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctaccg ccagctaaac
    38521 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aagaaggaga
    38581 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
    38641 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
    38701 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagctaccg ctggtcctgc
    38761 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
    38821 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
    38881 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
    38941 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
    39001 cagatggcga agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
    39061 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
    39121 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
    39181 cagaacggcg tatggagctg tactttcgtg ggctactgct ccgaagtctg cccgaaacac
    39241 gtcgatccgg ctgcggccat tcaacagggc aaagtagaaa gttcgaaaga ctttcttatc
    39301 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
    39361 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
    39421 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
    39481 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttattgt
    39541 gatcattaac ctgatcactc tggcggcagc tctgctgcac accaaaacct ggtttgaact
    39601 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
    39661 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
    39721 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
    39781 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
    39841 tggtgggtat tctgctgccg ctggggctgt ttccgggtga tgcactgagc tacgagcgcg
    39901 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
    39961 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtac
    40021 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
    40081 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
    40141 tgtatggaaa ccagacccta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
    40201 ttgctccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
    40261 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
    40321 ttattacttt acctgggggt atgcggacat cgccaaaaag cagcccgtca cacagcaaac
    40381 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
    40441 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
    40501 taaacagtgg aatgggatca cactattaca tctcgcaacc tacactgctg gcggcctgcc
    40561 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
    40621 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
    40681 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
    40741 cttccagcca ctcaaactca accatacgtg gattaatgta ccgcccgcag aagaaaagaa
    40801 ttacgcctgg ggatatcgcg aaggcaaggc agttcatgtt tcgccagggg cgttagatgc
    40861 tgaaacttat ggtgtgaagt cgaccattga agatatggcc tgctgggtac gaagcaatat
    40921 gaatccccgt gatatcaacg acaaaacact tcagcaaggg atacaactgg cacaatctcg
    40981 ctactggcaa accggcgata tgtatcaggg tctgggctgg gaaatgctgg actggccggt
    41041 aaatcctgac atcatcgtta acggcagtga caataaaatt gcactggcag cacgccccgt
    41101 aaaagcgatt acgcctccaa ctcctgcagt acgagcatca tgggtgcata aaacgggggc
    41161 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
    41221 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctggc agattctcaa
    41281 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
    41341 ctgtcatcta cacttagaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt
    41401 tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg
    41461 cgtgaccgta gtgaataatt tcgacgctaa acgctatctc ggtacctggt atgagattgc
    41521 ccgttttgat caccgctttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg
    41581 tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca
    41641 gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc
    41701 attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca
    41761 tgcgctggtt tgcggaccgg accgcgacta cctgtggata ctctcccgca cgccaaccat
    41821 ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag
    41881 taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat
    41941 ccccaatacg attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag
    42001 cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt
    42061 ccctactggt aacgatttca tcgcccaggc aagtagcgcc atactgacaa tcatcgccgt
    42121 cacggtaata acactcggcg tcaaacgact aaagccgtgg gtatatttca ggcctacggc
    42181 ccataccact tccagcagac cagcaataac taagataatc caggacatat caggctccgg
    42241 agaattgggg ccgtccccgg tgaaaagacg cgtttgcagg tcgtcctgca aagctgatgg
    42301 ttgaacggat attttgccag gtggcggggg aatttcaact attacctttg tgactatcgc
    42361 agaaaaaaca aacggcacga cacagctgtc gtaccgttat tgcttattgc tgcgctttcg
    42421 ttgctgcacc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg
    42481 tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct
    42541 tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa
    42601 taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa
    42661 ttagctggca gcgcgggaga tggagttgcc gagatgtttg atgtcttcgc cgaaaccgcg
    42721 agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt
    42781 catcatctct gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta
    42841 cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg
    42901 tacgaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt
    42961 acctgcggta tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt
    43021 cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc
    43081 cagcagccat ttagcgttgt cgccaattgc ttttgcgtca gcagacagct gctcgaaagt
    43141 ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc
    43201 gacaacatca gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc
    43261 ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa
    43321 ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa
    43381 atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc
    43441 acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac
    43501 ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg
    43561 aaaaactgtt ggccggacgc aacgccaaaa agctgtttgc cctgcgtgtg ccccgctcat
    43621 ttatcgatcg catggagaaa ggcaatccgg acgatccgct tttgcgtcag gtacttacct
    43681 cgcaagatga gtttgtcgtc gcgcccggat tctccaccga cccgctggaa gaacagcaca
    43741 gcgtagtgcc tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg
    43801 gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg
    43861 gcaacaagcg taactggcaa actgcacttg agtatgttgc tgcgcatccg gaactggacg
    43921 agatgatttt ctccggcggc gatccgctga tggcgaaaga tcacgagctg gactggttgc
    43981 tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagt cgtctgccga
    44041 ttgtgatccc ggcacgcatc acagatgcgc tggttgaacg cttttcacat tctacgctgc
    44101 aaatcttgct ggtgaatcac atcaaccatg ccaatgagat agatgaaaca ttccgtcagg
    44161 cgatggctaa gttgcgccgg gcgggcgtta ctttgctgaa ccagagcgtt ctgttacgtg
    44221 gtgtgaacga taacgcacaa acgctggcaa acctgagtaa tgcgttgttc gatgcaggcg
    44281 tgatgccgta ttacctgcat gtgctcgata aagtacaggg cgcggcgcat tttatggtga
    44341 gtgatgacga agcacggcag attatgcgtg agttgctgac actggtgtcg ggatatctgg
    44401 tgccgaaact ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc
    44461 tacgccagca gtaactgatt aaacagggaa gtttaatctt tgcccgttac ataaccttta
    44521 attattcata tacattgctg ttgttactct ttatataacc aacaccaata attaaccttt
    44581 cttttctgtt tatatagagt gagtacggct atttatgtta ttttaacaac tattactacc
    44641 aagcccattt atctggctta taaattaaaa ttaattattt aattgagaaa tggttaggga
    44701 gaacctacat ggccataagc atcaagggtg ttaataccgg tgttattcgc aagagtaata
    44761 atttcattgc actggcgctg aaaattaaag aacctcgcaa taaagaatcg ttgttcttta
    44821 tgtctgtcat ggaactgcgc gacctgctga ttgcgctgga aagtcgtctg catcaaaagc
    44881 ataaactgga tgctgctgca cgccttcagt atgagcaagc tcgcgataaa gtcattaaga
    44941 aaatggcaga aaacatccca gaaattctgg ttgatgagct taaaaatgcc gatattaatc
    45001 gtcgggttaa cacgctggaa cttaccgata atcaaggcga aaatcttacc ttcgtattaa
    45061 cgctacatga cggtagcaaa tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg
    45121 cgcgagcaat cattcatgca attaataatg cggaaatgcg tgaactggca ttacgtatta
    45181 cctcactgtt agatttcctg ccgttatatg acgttgactg ccagcaaaat ggcaatctgg
    45241 aatatgacac ctattcccaa ccggaatgga aacataactt gttcgaccat tatctggcgg
    45301 tgctttaccg ctttaaagat gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa
    45361 cgcgtgaagc aacgccgggt aaagaaatag aggcgatcac tcgccgaatg cttgatttca
    45421 gcccacgact gaaaaaactc gtgggtgtgc catgtcaggt ctatgttcgc accgtagcgg
    45481 cgaataacgc ccaaccgcta acccaggatc aatgcttacg cgcgctgcat cacttacgtg
    45541 ttcaatccac cagcaaaact gcaccacagg cgaaataatc tcatctttgc taatgcgagg
    45601 ctggagccat ccagcctgaa atgactgata acgtgatcgt tggtcatgcc ggatgcggcg
    45661 taaacgcctt atccggccta caaattctca agcattcaat cagttgcaat gccttatgta
    45721 ggcctgataa gcgaagcgca tctggcaatt atacgtttgt caccatcaca tcagttcggg
    45781 cacttatata cctggccgat aatctggcta tccgtcggga caaaactgga caacattccc
    45841 tgcgacgggc tactgatgcc ataaatcacg ttaccgccca ttgcagccgc ctggttgcgc
    45901 agatcgtttg ctgcgccgcg catagaaccg ccctcttctc cgtgttgccc ggaaagccag
    45961 ttgctttgct tacctgtcgc agtaccaatc agctggcact ctgcgcctgg ctgctcgtcc
    46021 acaatgcgta cactctgacc ggcagcactc aattcgttgc tggagctaca acccgccatc
    46081 agtagcgcgg caccgacaat ccctgctaag tattttacgt gcatgttatt ccccataatc
    46141 aatgagctgg acgccactcg cgtcgtccgt gtctgaatct tatactagaa agatgaccaa
    46201 aagaaaaacc cccagacatt tctgcccggg ggtttgttta tttctgcgag gtgcagggca
    46261 attacatcat gccgcccatg ccacccatgc cacccatgcc gccagcagcg cctaagtcag
    46321 ctgcatcgtt tttcggcagg tcggtaacca tgcattcggt ggtgatcatc aggccagcca
    46381 cagaagccgc gtactgcaga gcagaacggg ttactttggt tgggtccagg atacccatgt
    46441 cgatcatgtt gccgtattct tcggttgctg cgttgtaacc gtagttgccg tcgccgcctt
    46501 taacggtgtt agcaacaaca gacggttctt cgccgcagtt caggacgatc tgacgcagcg
    46561 gagcttccat tgcacgcagt gcaactttga tacccacgtt ctggtcttcg ttctgaccac
    46621 gcaggtcagc cagtttagac gctacgcgga tcagcgcaac accaccacca gcaaccacgc
    46681 cttcttctac cgcagcacgg gtcgcgtgca gggcgtcttc aacgcgtgct tttttctctt
    46741 tcatttcaac ttcggtagca gcacctactt tgataactgc aacgccgcct gccagtttcg
    46801 ctacgcgctc ctgcagtttt tcacggtcgt agtcagaagt tgcttcttca atctgctgac
    46861 ggatctgagc aacacggccc tggattgcag cttcttcgcc cacgccatcg atgatggtgg
    46921 tggtgtcttt gttgatcaca acgcgtttag cctgacccag gtcttccagg gttgcttttt
    46981 ccagctccat accgatctct tcagagatta cggtaccgcc agtcagggtt gcgatatcct
    47041 gcagcatagc tttacgacga tcgccgaagc ccggagcttt aactgcagca actttcacga
    47101 tgccacgcat ggtgttaaca accagagttg ccagcgcttc gccttctaca tcttcagcga
    47161 tgatcagcag cggtttgcct gctttggcaa cggcttccag aaccggcagc atttcgcgga
    47221 tgttggagat tttcttgtca gccagcagga tgaacgggct ttccagttct actgcgccag
    47281 tttccggctt gttgatgaag taaggagaca ggtagccacg gtcgaactgc ataccttcaa
    47341 ccacgtccag ttcgtcctgc agaccggtac cgtcttcaac ggtgataacg ccttctttac
    47401 cgactttgtc catcgcttca gcgatcagtt tacctacggt ttcgtcggag ttagcggaga
    47461 tagtaccaac ctgagcaatc gctttagagt cagagcacgg tacggacagc gctttcagtt
    47521 cttcaactgc agcggtaaca gctttgtcga taccacgttt caggtccatc gggttcatgc
    47581 ccgcagcaac agctttcaga ccttcagtga tgatagcctg agccagtacg gttgcagtgg
    47641 tggtaccgtc gcctgcagcg tcgttcgctt tagaggcaac ttctttcacc atctgcgcac
    47701 ccatgttttc gaacttgtct tccagttcga tttcacgagc aacggaaaca ccatctttgg
    47761 tgatggtcgg tgcaccgaaa gatttatcca gaactacgtt acggcctttc ggaccgaggg
    47821 taactttcac tgcatctgcc agtacgttta cgccgcgcag cattttcaca cgagcgtcgt
    47881 taccgaattt tacgtcttta gctgccatta tctttattcc ttaaattcgt atgttcagtg
    47941 tcgtgcgagg attacgcttc aacaattgcc agaatgtcgc tttcggacat gatcaacact
    48001 tcttcattgt cgatcttctc ggatttcaca ccgtagccat cgttgaaaat aacgatgtcg
    48061 ccaactttca catccagcgg cttcacttcg ccattttcaa ggatacggcc attgccgaca
    48121 gccagcactt cgccgcgggt ggatttagcc gctgcagagc cggtcagaac gatgccgcca
    48181 gcagatttag tttcaacttc tttacgcttg acgatcacgc gatcatgcaa tggacgaata
    48241 ttcattgata actctccttt gagaaagtcc gtatctgtta tgggtgacgc cggagcttac
    48301 gtggtttccc ggctggtgac cagagaaatg gggatgaggc ttcgcccctt caagggggaa
    48361 aaagaaaaaa aattctgatc agcacaaaat cgggtgaaaa ccctgattca cctcacattt
    48421 catcgcaatt tcttcatcgc cgtcataagc gaatctgatt gtgctaccat cgaaaatcta
    48481 cgcagttgcc caaaatttgg gcgcaatcgg acatcaaccc atgagtggac tcaaacaaga
    48541 actggggctg gcccagggca ttggcctgct atcgacgtca ttattaggca ctggcgtgtt
    48601 tgccgttcct gcgttagctg cactggtagc gggcaataac agcctgtggg cgtggcccat
    48661 tttgattatc ttagtgttcc cgattgcgat tgtgtttgcg attctgggtc gccactatcc
    48721 cagcgcaggc ggcgtcgcgc acttcgtcgg tatggcgttt ggttcgcggc ttgagcgagt
    48781 caccggctgg ctgtttttat cggtcattcc cgtgggtttg cctgccgcgc tacaaattgc
    48841 tgccggattc ggccaggcaa tgtttggctg gcatagctgg caactgttgt tggcagaact
    48901 cggtacgctg gcgttggtgt ggtatatcgg tactcgcggt gccagttcca gtgcaaatct
    48961 acaaacagtt attgccggac ttatcgtcgc actgattgtc gctatctggt gggcgggcga
    49021 tatcaaacct gcgagtatcc ccttccccgc gccaggaaat atcgaactta ccgggttatt
    49081 tgctgcgtta tcagtgatgt tctggtgttt tgtcggtctg gaagcatttg cccatcttgc
    49141 ctcggaattt aaaaatccag aacgtgattt tcctcgtgct ttgatgattg gtctgctgct
    49201 ggcaggatta gtctactggg gctgtacggt agtcgtctta cacttcgacg cctatggtga
    49261 acaaatggcc gcggcagcat cgcttccaaa aattgtagta caactgttcg gtgtaggagc
    49321 gttatggatt gcctgcgtaa ttggctatct ggcctgcttt gccagtctca acatttatat
    49381 acagagcttc gcccgcctgg tctggtcgca ggcgcaacat aatcctgacc actacctggc
    49441 acgcctctct tctcgccata tcccgaataa tgccctcaat gcggtgctcg gctgctgcgt
    49501 ggtgagcacg ttggtgattc atgctttaga gatcaatctg gacgctctta ttatttatgc
    49561 caatggcatc tttattatga tttatctgtt atgcatgctg gcaggctgta aattattgca
    49621 aggtcgttat cgactactgg cggtggttgg cgggctgtta tgcgttctgt tactggcaat
    49681 ggtcggctgg aaaagtctct atgcgctgat catgctggcg gggttatggc tgtttctgcc
    49741 aaaacgaaaa acgccggaaa atggcataac cacataatcc ggcgtttcga cattaatcct
    49801 ggcgatcgtc tttatgatca aggcggtcgc ggtcatcatc ctttcgctgg tactcaccat
    49861 caaaagtatt accgccaccg gtcccggcgc taaaaccgcc gcctggcatg cgagaaaagc
    49921 gcaaatgcgg catcaacttc actgtcagat gcttttgcac cggcggcaat aaaagcagaa
    49981 gaccgaggaa gtcggtgaaa aagcccggta ataaaagcag caaaccggca atgatcagcg
    50041 aaacactttt aatcatctcc gccgctgggt tttcacccgc cgccattttt tgctgcatca
    50101 gcacgaaatt cttaaagccc tggttacgta ccagtgacat accgataact gacgtaaata
    50161 taaccagcac gagagtcagc aataccccca atacatgggc aacctgaata aagattgaaa
    50221 tctcaatata gacataaagg aaaatggcaa taaaaggtaa ccagcgcaaa ggtttctcct
    50281 gtaatagcag ccggttaaac ccggctacct gaatgggttg cgaatcgcgt tcagcttata
    50341 ttgtggtcat tagcaaaatt tcaagatatt tgcgcaacta tttttggtag taatcccaaa
    50401 gcggtgatct atttcacaaa ttaataatta aggggtaaaa accgacactt aaagtgatcc
    50461 agattacggt agaaatcctc aagcagcata tgatctcgga tattcggtcg atgcagggga
    50521 taatcgtcgg tcgaaaaaca ttcgaaacca catatattct gtgtgtttaa agcaaatcat
    50581 tggcagcttg aaaaagaagg ttcacatgtc aaacaacatt cgtatcgaag aagatctgtt
    50641 gggtaccagg gaagttccag ctgatgccta ctatggtgtt cacactctga gagcgattga
    50701 aaacttctat atcagcaaca acaaaatcag tgatattcct gaatttgttc gcggtatggt
    50761 aatggttaaa aaagccgcag ctatggcaaa caaagagctg caaaccattc ctaaaagtgt
    50821 agcgaatgcc atcattgccg catgtgatga agtcctgaac aacggaaaat gcatggatca
    50881 gttcccggta gacgtctacc agggcggcgc aggtacttcc gtaaacatga acaccaacga
    50941 agtgctggcc aatatcggtc tggaactgat gggtcaccag aaaggtgaat atcagtacct
    51001 gaacccgaac gaccatgtta acaaatgtca gtccactaac gacgcctacc cgaccggttt
    51061 ccgtatcgca gtttactctt ccctgattaa gctggtagat gcgattaacc aattgcgtga
    51121 aggctttgaa cgtaaagcgg tcgaattcca ggacatcctg aaaatgggtc gtacccagct
    51181 gcaggacgca gtaccgatga ccctcggtca ggaattccgc gctttcagca tcctgctgaa
    51241 agaagaagtg aaaaacatcc aacgtaccgc tgaactgctg ctggaagtta accttggcgc
    51301 aacagcaatc ggtactggtc tgaacacgcc gaaagagtac tctccgctgg cagtgaaaaa
    51361 actggctgaa gttactggct tcccatgcgt accggctgaa gacctgatcg aagcgacctc
    51421 tgactgcggc gcttatgtta tggttcacgg agcgctgaaa cgcctggctg tgaagatgtc
    51481 caaaatctgt aacgacctgc gcttgctctc ttctggtcca cgtgccggcc tgaacgagat
    51541 caacctgccg gaactgcagg cgggctcttc catcatgcca gctaaagtaa acccggttgt
    51601 tccggaagtg gttaaccagg tatgcttcaa agtcatcggt aacgacacca ctgttaccat
    51661 ggcagcagaa gcaggtcagc tgcagttgaa cgttatggag ccggtcattg gccaggctat
    51721 gttcgaatcc gttcacattc tgaccaacgc ttgctacaac ctgctggaaa aatgcattaa
    51781 cggcatcact gctaacaaag aagtgtgcga aggttacgtt tacaactcta tcggtatcgt
    51841 tacttacctg aacccgttca tcggtcacca caacggtgac atcgtgggta aaatctgtgc
    51901 cgaaaccggt aagagtgtac gtgaagtcgt tctggaacgc ggtctgttga ctgaagcgga
    51961 acttgacgat attttctccg tacagaatct gatgcacccg gcttacaaag caaaacgcta
    52021 tactgatgaa agcgaacagt aatcgtacag ggtagtacaa ataaaaaagg cacgtcagat
    52081 gacgtgcctt ttttcttgtg agcagtaact taaaaataac aatctaatat caacttgtta
    52141 aaaaacaagg aaggctaata tgctagttgt agaactcatc atagttttgc tggcgatctt
    52201 cttgggcgcc agattggggg gaataggtat tggttttgca ggcggattgg gggtgctggt
    52261 tcttgccgct attggcgtta aacccggtaa catcccgttc gatgtcattt ccattatcat
    52321 ggcggttatc gccgctattt ctgccatgca ggttgctggc ggtctggact atctggttca
    52381 tcagacagaa aagctgctgc gccgtaaccc gaaatacatc acgatcctcg caccgatcgt
    52441 gacctatttc ctgactatct ttgctggtac tggcaacatc tctctggcga cactgccagt
    52501 tatcgctgaa gttgcgaagg aacaaggcgt taaaccttgc cgtccgctgt ctactgcagt
    52561 ggtatccgcg cagattgcga tcaccgcatc gccaatctca gcggcagtgg tttacatgtc
    52621 ttccgtgatg gaaggtcatg gcatcagcta cctccatctg ctctccgtgg tcatcccgtc
    52681 caccctgctg gcggttctgg tgatgtcctt cctggtcact atgctgttca actccaaact
    52741 ctctgacgat ccgatttatc gcaagcgtct ggaagagggc ctggttgaac tgcgcggtga
    52801 aaagcagatt gaaatcaaat ccggtgcaaa aacgtccgtc tggctgttcc tgctgggcgt
    52861 agttggcgtg gttatctatg caatcatcaa cagcccaagc atgggtctgg ttgaaaaacc
    52921 gctgatgaac accaccaacg caatcctgat catcatgctc agtgttgcaa ctctgaccac
    52981 cgttatctgt aaagtcgata ccgacaacat cctcaactcc agcaccttca aagcaggtat
    53041 gagcgcctgt atttgtatcc tgggtgttgc gtggctgggc gatactttcg tttccaacaa
    53101 catcgactgg atcaaagata ccgctggtga agtgattcag ggtcatccgt ggctgctggc
    53161 cgtcatcttc ttctttgctt ctgctctgct gtactctcag gctgcaaccg caaaagcact
    53221 gatgccgatg gctctggcac tgaacgtttc tccgctgacc gctgttgctt ctttcgctgc
    53281 ggtgtctggt ctgttcattc tgccgaccta cccgacgctg gttgctgcgg tacagatgga
    53341 tgacacgggt actacccgta tcggtaaatt cgtcttcaac catccgttct tcatcccggg
    53401 tactctgggt gttgccctgg ccgtttgctt cggcttcgtg ctgggtagct tcatgctgta
    53461 atgaccaatc gcggggcgtt cacgccccgc tttctttctc gccgactaac atcctttccc
    53521 cgtccgttgt atagtgacct ctctcttgcg gttccatctg ttcttgcgag gtgtttatgc
    53581 ttgatgaaaa aagttcgaat accgcgtctg tcgtggtgct atgtaccgca ccggatgaag
    53641 cgacagccca ggatttagcc gccaaagtgc tggcggaaaa actggcggcc tgcgcgacct
    53701 tgatccccgg cgctacctct ctctattact gggaaggtaa gctggagcaa gaatacgaag
    53761 tgcagatgat tttaaaaact accgtatctc accagcaggc actgctggaa tgcctgaagt
    53821 ctcatcatcc atatcaaacc ccggaacttc tggttttacc tgttacacac ggagacacag
    53881 attacctctc atggctcaac gcatctttac gctgatcctg ctactttgca gcacttccgt
    53941 ttttgccgga ttattcgacg cgccgggacg ttcacaattt gtccccgtgg atcaagcctt
    54001 tgcttttgat tttcagcaaa accaacatga ccttaatctg acctggcaga tcaaagacgg
    54061 ttactacctc taccgtaaac agatccgcat tacgccggaa cacgcgaaaa ttgccgacgt
    54121 gcagctgccg caaggcgtct ggcatgaaga tgagttttac ggcaaaagcg agatttaccg
    54181 cgatcggctg acgcttcccg taaccatcaa ccaggcgagt gcgggagcga cgttaactgt
    54241 cacctaccag ggctgtgctg atgccggttt ctgttatccg ccagaaacca aaaccgttcc
    54301 gttaagcgaa gtggtcgcca acaacgcagc gtcacagcct gtgtctgttc cgcagcaaga
    54361 gcagcccacc gcgcaattgc ccttttccgc gctctgggcg ttgttgatcg gtattggtat
    54421 cgcctttacg ccatgcgtgc tgccaatgta cccactgatt tctggcatcg tgctgggtgg
    54481 taaacagcgg ctctccactg ccagagcatt gttgctgacc tttatttatg tgcaggggat
    54541 ggcactgact tacacggcgc tgggtctggt agttgccgcc gcagggttac agttccaggc
    54601 ggcgctacag cacccatacg tgctcattgg cctcaccatc gtctttacct tgctggcgat
    54661 gtcaatgttt ggcttgctta ctctacaact cccctcctcg ctgcaaacgc gcctcacgct
    54721 gatgagcaat cgccaacagg gcggctcacc cggcggagtg tttattatgg ggacgattgc
    54781 cggactgatc tgttcaccat gcaccaccgc accgcttagc gcgattctgc tgtatatcgc
    54841 ccaaagcggg aacatgtggc tgggcggcgg cacgctttat ctctatgcgt tgggcatggg
    54901 cctgccgctg atgctaatta ccgtctttgg taaccgcttg ctgccgaaaa gcggcccgtg
    54961 gatggaacaa gtcaaaaccg cgtttggttt tgtgatcctc gcactgccgg tcttcctgct
    55021 ggagcgagtg attggtgatg tatggggatt acgcttgtgg tcggcgctgg gtgtcgcatt
    55081 ctttggctgg gcctttatca ccagcctaca ggccaaacgc ggctggatgc gcgtggtgca
    55141 aataatcctg cttgcagcgg cattggttag cgtgcgccca cttcaggatt gggcatttgg
    55201 tgcgacgcat accgcgcaaa ctcagacgca tctcaacttt acacaaatca aaacggtaga
    55261 tgagttaaat caggcgctcg ttgaagccaa aggcaaaccg gtgatgttag atctttatgc
    55321 cgactggtgc gtcgcctgta aagagtttga gaaatacacc ttcagcgacc cgcaggtgca
    55381 aaaagcgtta gcagacacgg tattacttca ggccaacgtc actgccaacg acgcacaaga
    55441 tgtggcgctg ttaaagcatc ttaatgtcct tggcctaccg acaattctct tttttgacgg
    55501 acaaggccag gagcatccac aagcacgcgt cacgggcttt atggatgctg aaaccttcag
    55561 cgcacatttg cgcgatcgcc aaccgtgaac gacacttgca gtgggaaaga cggaggaaaa
    55621 tagcgtgcaa cgtgaagatg tactgggaga agccctgaaa ttattagaat tacaagggat
    55681 tgccaacacc acgctggaga tggttgctga acgtgtggat tatccactgg acgagctacg
    55741 ccgcttctgg ccagacaaag aggcaatcct ctacgatgcg ctgcgttatc ttagccaaca
    55801 gatagatgtc tggcgtcgtc agctgatgct ggacgaaacg caaaccgccg aacaaaagct
    55861 gctggcacgt tatcaggcgt tatcggagtg cgttaaaaac aaccgctatc cgggctgtct
    55921 gtttatcgct gcctgtacgt tttatcccga tcctggccac cctattcatc aactggccga
    55981 tcagcaaaaa agcgcggcct acgatttcac ccacgaactg ttaaccacgc tggaagttga
    56041 cgatccggcg atggtagcaa agcagatgga actggtgctg gaaggctgtt taagccgaat
    56101 gctggtgaat cgtagccagg cggatgtcga caccgcacat cggctggcgg aagatatcct
    56161 gcgcttcgcc cgctgccgcc agggtggtgc actgacctga cagaaacaca gaaaagaagc
    56221 gatttgccgc aatcttaagc agttgaatcg cttttactga aattaggttg acgagatgtg
    56281 cagattacgg tttaatgcgc cccgttgccc ggatagctca gtcggtagag caggggattg
    56341 aaaatccccg tgtccttggt tcaattccga gtccgggcac caaattcata taaacggacc
    56401 tccacggagg tccgtttttc gtttcaggac gccacgattt aagtgttctg cctccaaatc
    56461 aattctaccg aactcaaccc gattctcccc acatcaccag caatttgcgg gcatatccca
    56521 attcgggaaa atttgttcct gagctatagc actaactaac gtgaaatgtc gtgcggcccc
    56581 gtgatgctgt tgaacgtcaa atgacgtcat caggagcgta acgcatccat aaagcgcaac
    56641 atcggtctga acgccgactg atgagatttt ctgaatgaga acaaagagaa atgtatcagt
    56701 ccgcttgctc atgcaaagac taacaatcca ttaaaatagt aagcgctccg gacaattttc
    56761 catggattat tttctgaaca tttttctttg gcaaagatga tgaattttga tggtaaggaa
    56821 tattacttct ggttctcagt aaaatccttt cgtaatacca tgtaatcaag aagtttatgg
    56881 ctggtaaaaa taacgtcttg cattcaccaa taatatgtaa ataaacccat ctatagatgg
    56941 taaaaatagg ttgtggcaat tatcattgca tcattccctt ttcgaatgag tttctattat
    57001 gcaacaacct gtagttcgcg ttggcgaatg gcttgttact ccgtccataa accaaattag
    57061 ccgcaatggg cgtcaactta cccttgagcc gagattaatc gaccttctgg ttttctttgc
    57121 tcaacacagt ggcgaagtac ttagcaggga tgaacttatc gataatgtct ggaagagaag
    57181 tattgtcacc aatcacgttg tgacgcagag tatctcagaa ctacgtaagt cattaaaaga
    57241 taatgatgaa gatagtcctg tctatatcgc tactgtacca aagcgcggct ataaattaat
    57301 ggtgccggtt atctggtaca gcgaagaaga gggagaggaa ataatgctat cttcgcctcc
    57361 ccctatacca gaggcggttc ctgccacaga ttctccctcc cacagtctta acattcaaaa
    57421 caccgcaacg ccacctgaac aatccccagt taaaagcaaa cgattcacta ccttttgggt
    57481 atggtttttt ttcctgttgt ccttaggtat ctgtgtagca ctggtagcgt tttcaagtct
    57541 tgatacacgt cttccgatga gcaaatcgcg tattttgctc aatccacgcg atattgacat
    57601 taatatggta aataaaagtt gtaacagctg gagttccccg tatcagctct cttacgcgat
    57661 aggcgtgggt gatttggtgg cgacatcact taacaccttc tccaccttta tggtgcatga
    57721 caaaatcaac tacaacattg atgaaccgag cagttccggt aaaacattat ctattgcgtt
    57781 tgttaatcag cgccaatacc gtgctcaaca atgctttatg tcgataaaat tggtagacaa
    57841 tgcagatggt tcaaccatgc tggataaacg ttatgtcatc actaacggta atcagctggc
    57901 gattcaaaat gatttactgg agagtttatc aaaagcgtta aaccaaccgt ggccacaacg
    57961 aatgcaggag acgctccagc aaattttgcc tcatcgtggt gcgttattaa ctaattttta
    58021 tcaggcacat gattatttac tgcatggcga tgataaatca ttgaaccgtg ccagtgaatt
    58081 attaggtgag attgttcaat catccccaga atttacctac gcgagagcag aaaaagcatt
    58141 agttgatatc gtgcgccatt ctcaacatcc tttagatgaa aaacaattag cagcactgaa
    58201 cacagaaata gataacattg ttacactacc ggaattgaat aacctgtcca ttatatatca
    58261 aataaaagcg gtcagtgctc tggtaaaagg taaaacagat gagtcttacc aggcgataaa
    58321 tactggcatt gatcttgaaa tgtcctggct aaattatgta ttgcttggca aggtttatga
    58381 aatgaagggg atgaaccggg aagcggctga tgcatatctc accgccttta atttacgccc
    58441 aggggcaaac accctttact ggattgaaaa tggtatattc cagacttctg ttccttatgt
    58501 tgtaccttat ctcgacaaat ttctcgcttc agaataagta acccccgggt tgattcatgc
    58561 tcgggaatat ttgttgttga gtttttgtat gttcctgttg gtataatatg ttgcggcaat
    58621 ttatttgccg cataattttt attacataaa tttaaccaga agacgtcacg caatctattg
    58681 taaacattga atgtttatct tttcatgata tcaacttgcg atcctgatgt gttaataaaa
    58741 aacctcaagt tctcacttac agaaactttt gtgttatttc acctaatctt taggattaat
    58801 ccttttttcg tgagtaatct tagcgccagt ttggtctggt caggaaatag ttatacatca
    58861 tgacccggac tccaaattca aaaatgaaat taggagaaga gcatgagttc tgccaagaag
    58921 atcgggctat ttgcctgtac cggtgttgtt gccggtaata tgatggggag cggtattgca
    58981 ttattacctg cgaacctagc aagtatcggt ggtattgcta tctggggttg gattatctct
    59041 attattggtg caatgtcgct ggcgtatgta tatgcccgac tggcaacaaa aaacccgcaa
    59101 caaggtggcc caattgctta tgccggagaa atttctcctg catttggttt tcagacaggt
    59161 gttctttatt accatgctaa ctggattggt aacctggcga ttggtattac cgctgtatct
    59221 tatctttcca ccttcttccc agtattaaat gatcctgttc cggcgggtat cgcctgtatt
    59281 gctatcgtct gggtatttac ctttgtaaat atgctcggcg gtacctgggt aagccgttta
    59341 accactattg gtctggtgct ggttcttatt cctgtggtga tgactgctat tgttggctgg
    59401 cattggtttg atgcggcaac ttatgcagct aactggaata ctgcggatac cactgatggt
    59461 catgcgatca ttaaaagtat tctgctctgc ctgtgggcct tcgtgggtgt tgaatccgca
    59521 gctgtaagta ctggtatggt taaaaacccg aaacgtaccg ttccgctggc aaccatgctg
    59581 ggtactggtt tagcaggtat tgtttacatc gctgcgactc aggtgctttc cggtatgtat
    59641 ccgtcttctg taatggcggc ttccggtgct ccgtttgcaa tcagtgcttc aactatcctc
    59701 ggtaactggg ctgcgccgct ggtttctgca ttcaccgcct ttgcgtgcct gacttctctg
    59761 ggctcctgga tgatgttggt aggccaggca ggtgtacgtg ccgctaacga cggtaacttc
    59821 ccgaaagttt atggtgaagt cgacagcaac ggtattccga aaaaaggtct gctgctggct
    59881 gcagtgaaaa tgactgccct gatgatcctc atcactctga tgaactctgc cggtggtaaa
    59941 gcatctgacc tgttcggtga actgaccggt atcgcagtac tgctgactat gctgccgtac
    60001 ttctactctt gcgttgacct gattcgtttt gaaggcgtta acatccgcaa ctttgtcagc
    60061 ctgatctgct ctgtactggg ttgcgtgttc tgcttcatcg cgctgatggg cgcaagctcc
    60121 ttcgagctgg caggtacctt catcgtcagc ctgattatcc tgatgttcta cgctcgcaaa
    60181 atgcacgagc gccagagcca ctcaatggat aaccacaccg cgtctaacgc acattaatta
    60241 aaagtatttt ccgaggctcc tcctttcatt ttgtcccatg tgttgggagg ggcctttttt
    60301 acctggagat atgactatga acgttattgc aatattgaat cacatggggg tttattttaa
    60361 agaagaaccc atccgtgaac ttcatcgcgc gcttgaacgt ctgaacttcc agattgttta
    60421 cccgaacgac cgtgacgact tattaaaact gatcgaaaac aatgcgcgtc tgtgcggcgt
    60481 tatttttgac tgggataaat ataatctcga gctgtgcgaa gaaattagca aaatgaacga
    60541 gaacctgccg ttgtacgcgt tcgctaatac gtattccact ctcgatgtaa gcctgaatga
    60601 cctgcgttta cagattagct tctttgaata tgcgctgggt gctgctgaag atattgctaa
    60661 caagatcaag cagaccactg acgaatatat caacactatt ctgcctccgc tgactaaagc
    60721 actgtttaaa tatgttcgtg aaggtaaata tactttctgt actcctggtc acatgggtgg
    60781 tactgcattc cagaaaagcc cggtaggtag cctgttctat gatttctttg gtccgaatac
    60841 catgaaatct gatatttcca tttcagtatc tgaactgggt tctctgctgg atcacagtgg
    60901 tccacacaaa gaagcagaac agtatatcgc tcgcgtcttt aacgcagacc gcagctacat
    60961 ggtgaccaac ggtacttcca ctgcgaacaa aattgttggt atgtactctg ctccggcagg
    61021 cagcaccatt ctgattgacc gtaactgcca caaatcgctg acccacctga tgatgatgag
    61081 cgatgttacg ccaatctatt tccgcccgac ccgtaacgct tacggtattc ttggtggtat
    61141 cccacagagt gaattccagc acgctaccat tgctaagcgc gtgaaagaaa caccaaacgc
    61201 aacctggccg gtacatgctg taattaccaa ctctacctat gatggtctgc tgtacaacac
    61261 cgacttcatc aagaaaacac tggatgtgaa atccatccac tttgactccg cgtgggtgcc
    61321 ttacaccaac ttctcaccga tttacgaagg taaatgcggt atgagcggtg gccgtgtaga
    61381 agggaaagtg atttacgaaa cccagtccac tcacaaactg ctggcggcgt tctctcaggc
    61441 ttccatgatc cacgttaaag gtgacgtaaa cgaagaaacc tttaacgaag cctacatgat
    61501 gcacaccacc acttctccgc actacggtat cgtggcgtcc actgaaaccg ctgcggcgat
    61561 gatgaaaggc aatgcaggta agcgtctgat caacggttcc attgaacgtg cgatcaaatt
    61621 ccgtaaagag atcaaacgtc tgagaacgga atctgatggc tggttctttg atgtatggca
    61681 gccggatcat atcgatacga ctgaatgctg gccgctgcgt tctgacagca cctggcacgg
    61741 cttcaaaaac atcgataacg agcacatgta tcttgacccg atcaaagtca ccctgctgac
    61801 tccggggatg gaaaaagacg gcaccatgag cgactttggt attccggcca gcatcgtggc
    61861 gaaatacctc gacgaacatg gcatcgttgt tgagaaaacc ggtccgtata acctgctgtt
    61921 cctgttcagc atcggtatcg ataagaccaa agcactgagc ctgctgcgtg ctctgactga
    61981 cttcaaacgt gcgttcgacc tgaacctgcg tgtgaaaaac atgctgccgt ctctgtatcg
    62041 tgaagatcct gaattctatg aaaacatgcg tattcaggaa ctggctcaga atatccacaa
    62101 actgattgtt caccacaatc tgccggatct gatgtatcgc gcatttgaag tgctgccgac
    62161 gatggtaatg actccgtatg ctgcattcca gaaagagctg cacggtatga ccgaagaagt
    62221 ttacctcgac gaaatggtcg gtcgtattaa cgccaatatg atccttccgt atccgccggg
    62281 agttcctctg gtaatgccgg gtgaaatgat caccgaagaa agccgtccgg ttctggagtt
    62341 cctgcagatg ctgtgtgaaa tcggcgctca ctatccgggc tttgaaaccg atattcacgg
    62401 tgcataccgt caggctgatg gccgctatac cgttaaggta ttgaaagaag aaagcaaaaa
    62461 ataattagct cgtacaaggg aagtggcttg ccacttccct tttttgctca taaggagaac
    62521 acatgaaaac accctcacag ccgcgcgcga tatactatat cgtggcgatc caaatctggg
    62581 agtacttcag tttttacggc atgcgtgcct tactcattct ctatctcacc catcagcttg
    62641 gttttgatga taaccatgcc atcagcctgt tcagcgcata tgcttctctg gtttacgtta
    62701 cccctattct cggcggctgg cttgccgacc gcctgctcgg caaccgcact gcagtgattg
    62761 ccggcgcgct gttaatgacc cttggccatg tggtgctggg tattgataca aattcaacct
    62821 ttagcctgta tctggcgctg gcaatcatta tttgtggcta cggtttattc aaatcaaaca
    62881 tcagctgttt gcttggcgag ctctacgacg agaacgatca tcgacgtgat ggcggttttt
    62941 cgctgctgta tgctgcgggc aatatcggtt ctatcgccgc cccaatcgcc tgcggcctgg
    63001 ctgctcagtg gtatggatgg catgttggct ttgcccttgc gggtggcggc atgtttatcg
    63061 gtttgttgat tttcttaagc gggcatcgtc atttccagtc cacgcgtagt atggataaaa
    63121 aagcgctcac tagcgtcaaa tttgccttac cggtatggag ctggttagtg gtgatgctct
    63181 gtttagcccc agtatttttt accctgctgc tggagaacga ctggtccgga tatttgctgg
    63241 cgatcgtttg cctcattgcc gcacaaatca ttgcccgcat gatgatcaaa ttccccgaac
    63301 accgccgtgc tctttggcaa attgtattgt tgatgtttgt cgggacattg ttctgggtac
    63361 tggcacaaca gggcggcagt accatcagct tgtttatcga tcgctttgtg aatcgtcagg
    63421 cattcaatat tgaagtacct actgcactat tccagtcggt gaatgcaatt gcggtgatgc
    63481 tcgctggggt tgttctggcc tggctggcgt cgccagaaag ccacggcaac tcaacattgc
    63541 gcgtctggct gaagtttgcc tttggcttac tgctgatggc ttgtggcttt atgttgctgg
    63601 catttgatgc ccgacatgca gcagctgacg gtcaagcgtc aatgggcgtg atggtatccg
    63661 ggctggcgct aatgggcttt gccgaactct ttattgaccc ggtggcgatt gcgcaaatca
    63721 cgcgtctgaa aatgtctggc gtattaaccg ggatttatat gctggcaaca ggcgcggtcg
    63781 ccaactggct ggcaggcgta gtggcacagc agacgacaga gtcgcaaatt agcggtatgg
    63841 caattgcagc ttaccagcga ttcttttctc agatgggaga gtggacgttg gcttgtgtcg
    63901 cgatcatcgt ggtattggcc tttgctaccc gtttcctgtt tagcacgccg acgaatatgg
    63961 tacaggagag caacgattaa ctgtcgtaaa aacaattggt tatgaataaa ttaactttca
    64021 gtttatcaca ctagtaaatc gagattaata agtcatttaa accattttga tggttattta
    64081 ttagtgatat caacttgagg taagcgttag tttcgataag ataaactgag ttattaatag
    64141 tcgaggcaga taatacagtg taccgatctg tctctttatc tacgctaaat tgaaagctgg
    64201 atttagagga accaaaatgt ctgaacaaga aacacgggga gccaatgagg ctattgattt
    64261 taacgatgaa ctgagaaatc gccgcgaaaa actggcggca ctacgtcagc aaggtgtggc
    64321 gtttcccaat gattttcgcc gcgaccatac ctctgaccag ttgcacgaag agtttgatgc
    64381 gaaggataac caggaactgg aatccttaaa cattgaagtc tcggttgctg gccgaatgat
    64441 gacccgtcgt atcatgggga aagcctcctt tgtaacgttg caggatgtcg gtggccgtat
    64501 tcaactgtac gtcgcacgag atagcctgcc agaaggtgtt tataacgatc agtttaaaaa
    64561 atgggatctg ggtgacatta tcggtgcccg cggtacgctg tttaagacgc aaacgggtga
    64621 gctttccatt cactgtactg agttgcgcct gctgactaaa gcactacgtc ctttaccaga
    64681 taaattccat ggtctgcagg atcaggaagt ccgttatcgt caacgttatc tggacctcat
    64741 cgctaacgat aaatcccgtc aaacgtttgt tgtccgttca aaaattctgg ccgctatccg
    64801 ccagttcatg gtcgcgcgcg gctttatgga agtagaaacc ccgatgatgc aggtaattcc
    64861 aggtggggca tctgctcgcc cgtttattac ccatcataat gctctggatt tagatatgta
    64921 tctgcgtatc gcgccggagc tgtatctgaa acgtctggtt gtaggcggtt ttgaacgggt
    64981 attcgaaatc aaccgtaact tccgtaatga aggtatttct gttcgccata atcctgagtt
    65041 cacaatgatg gaactctaca tggcgtatgc ggattaccac gatttgattg aactgacaga
    65101 gtccctgttc cgcaccctgg cacaagaggt tctgggtacc actaaagtca cttatggcga
    65161 gcatgtgttt gatttcggca aaccgtttga aaaactcacc atgcgcgaag caatcaaaaa
    65221 atatcgtcca gaaaccgata tggccgacct ggataatttt gatgctgcta aagcattagc
    65281 tgaatctatc ggtattacgg tagagaaaag ctgggggctg ggacgtattg tcacggagat
    65341 ctttgatgaa gtggcagaag cacatctgat tcagccaacc tttattacgg aatatccggc
    65401 agaagtgtca ccgctggcac gccgtaatga tgttaacccg gaaatcaccg accgttttga
    65461 attcttcatc ggtggtcgtg aaatcggtaa tggttttagc gaattaaacg acgcagaaga
    65521 tcaggctgaa cgtttccagg aacaggttaa cgctaaagcc gcaggtgacg acgaagccat
    65581 gttctatgac gaagattacg tgactgcgct ggaatatggt ctgccgccaa ccgctggtct
    65641 gggtattggt atcgaccgaa tgattatgct gtttactaac agccatacta ttcgcgacgt
    65701 tattctcttc ccggcgatgc gcccacagaa ataaatttca ctttaatgaa cgaagcagtc
    65761 aggcgactgc ttcgttcatc gttccgcaaa tccagttttg aggagagacg cttcgtttca
    65821 cgataaccag cgggtccact caactacagc tcctaaaaga gagaaaaaag taatgccaca
    65881 ggcagactca ttggccaggt tattccgacc aggaatgcac taagtaaacg aatatgtgtt
    65941 ttctcatgtg agataaacca gataatgaca aaggatatgt tcacaccaat cacataaaaa
    66001 attaatattt tagagaatag tgccattttt tatgttcttc ttatccttac tggctacttg
    66061 taaaactgac ggaaatagtc atcaactgtc ataatgttga ggtcaaagtc aatatccggt
    66121 aagtctaagc atttaagcct gattaaatct tcgatttcac ttgcggctaa cgatgtcgtt
    66181 attgcgaagg aattttcatt caattgtcgt ggtatcccat attcatcagc cacgatagaa
    66241 gagaaattca tatcatacat caatgatctt aaccttaaga acactgagga ataagatgat
    66301 ggataatcaa aagaaacaac ataagtattg aacttgtttt taccttccat aatatgactc
    66361 ctataaatat tgaacactct ggggacgcaa aatttattaa ccccgattga tgattctcat
    66421 ataggagata ttcttttttt tgctcagtaa aggggatgac ttgggacaaa aaaacgcgct
    66481 ctctgcccat tctttgtgca tccccctacc cttcagatgc tctcctgcat cacaccgcgg
    66541 agagcatgaa aaatatcgtg aatctgtact cttccatcaa ctgcgaatat catcatactc
    66601 ccgcgtttta tcaaattcgt gtttcgcaaa tggacataat gggataattt ttcgtttttc
    66661 ccgacgcatt ttttccacga ctttcgcaac cagctgttta ccaatccctt gccctttcag
    66721 gctttcatcg acatcggtat gttcgataat cgctaaattc tctccggtcg gcacaaagac
    66781 aatttcagcg atttgcttgc cttgttcgtc attaatgtaa aatttattgt ggccttcgcg
    66841 tatttccatt ttaccctcgc ttatttatga cggtatttca gcgcaccact cgggcacgta
    66901 tcaatcactt taaccacagt ggcaacgtcg acttcatccg gcatgatcca cggctttcgt
    66961 ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg acatatcgca
    67021 gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc gtccagtagc
    67081 gcctgatcca tgaccttccc tctctcatca gtatggtttt cactataagc atagccctgg
    67141 aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat atagccaatc
    67201 attcttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa atttgtcaca
    67261 caaggaagct gatgagacat tcattgccct accgcatgtt acgcaaacgt ccgatgaaat
    67321 tgagtaccac agtgatctta atggtcagcg cggtactgtt ctcggtgcta ttggtggtgc
    67381 atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctagcc aacaaggcac
    67441 tggcagtggc gcgtactctc gccgactcgc cggaaatccg tcagggcttg cagaaaaaac
    67501 cgcaggagag cggcatccag gccatcgcgg aagccgtgcg caaacgcaac gatctgctgt
    67561 ttattgtcgt taccgatatg caaagtcttc gctactcgca tcctgaagcc cagcgtattg
    67621 gtcagccatt taaaggtgat gacatcctta aagcgctgaa tggcgaagaa aatgtcgcta
    67681 tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac gatgaaaatc
    67741 ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc caacagatca
    67801 atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc ggactgattg
    67861 gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa ccctacgaaa
    67921 tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa ggcgtcgttg
    67981 ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa ttgctgaatt
    68041 accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg tcacaggtgg
    68101 tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag attacgatta
    68161 aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt atcatcggtg
    68221 ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga ctcgacggtc
    68281 tggtcaacta tgctgatgca cttcgtgaac gatcccacga atttatgaat aaattgcatg
    68341 tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac attctcaaaa
    68401 cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa tctccggtta
    68461 tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat acgctgattt
    68521 taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg acgctgatta
    68581 ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa cccggtggcg
    68641 aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt aacgatgatg
    68701 gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc tcgacaaaag
    68761 gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat ctcggcggca
    68821 gcatcgccgt ggaatcggaa cccgggattt tcacacaatt ttttgtccag ataccctggg
    68881 acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg caatggtcgc
    68941 ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg gaacagcctc
    69001 gacgctggag aaagccaaag agattatctt caatagcgat acgcctatcg acctgatatt
    69061 gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc tgcataacgc
    69121 gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa ccattaaaga
    69181 ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt cccgctttga
    69241 agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc agtattatga
    69301 ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgagcagg atcctcgtcg
    69361 cttgccaaaa ggcttaacgc cgcagacgtt acgcacactg tgccagtgga ttgacgcgca
    69421 tcaggactat gagttttcaa ccgacgagct agccaacgag gttaacattt cgcgtgtttc
    69481 ctgccgtaaa taccttatct ggctggtcaa ctgccacatc ttgttcacca gtatccatta
    69541 tggcgtcacg gggcgtccag tttaccgtta ccgtattcag gcagaacact actcactact
    69601 gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta ttaccgtaat
    69661 gc
//
LOCUS       DAZKIF010000007        68562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZKIF010000007 DAZKIF010000000
VERSION     DAZKIF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 68562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.007"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..916
                     /locus_tag="ACHOTW_000517"
     CDS             <1..916
                     /locus_tag="ACHOTW_000517"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFP9842688.1"
                     /translation="PLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVLLN
                     DKNNELSDGTNFWDKNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEV
                     ALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRALGH
                     EKENSLKRIINSFNSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQYLSQSCGAFV
                     CMAAQEVIEQMESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLENRNCYLDKYG
                     DANINHYYRNLEIKNSHPKNRASSKRVS"
     gene            1328..1945
                     /locus_tag="ACHOTW_000518"
                     /pseudo
     CDS             1328..1945
                     /locus_tag="ACHOTW_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2048..2551
                     /gene="yfbM"
                     /locus_tag="ACHOTW_000519"
     CDS             2048..2551
                     /gene="yfbM"
                     /locus_tag="ACHOTW_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP9842689.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(2618..4075)
                     /gene="nuoN"
                     /locus_tag="ACHOTW_000520"
     CDS             complement(2618..4075)
                     /gene="nuoN"
                     /locus_tag="ACHOTW_000520"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP9842690.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(4082..5611)
                     /gene="nuoM"
                     /locus_tag="ACHOTW_000521"
     CDS             complement(4082..5611)
                     /gene="nuoM"
                     /locus_tag="ACHOTW_000521"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP9842691.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5842..7683)
                     /gene="nuoL"
                     /locus_tag="ACHOTW_000522"
     CDS             complement(5842..7683)
                     /gene="nuoL"
                     /locus_tag="ACHOTW_000522"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP9842692.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7680..7982)
                     /gene="nuoK"
                     /locus_tag="ACHOTW_000523"
     CDS             complement(7680..7982)
                     /gene="nuoK"
                     /locus_tag="ACHOTW_000523"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP9842693.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7979..8533)
                     /gene="nuoJ"
                     /locus_tag="ACHOTW_000524"
     CDS             complement(7979..8533)
                     /gene="nuoJ"
                     /locus_tag="ACHOTW_000524"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP9842694.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(8545..9087)
                     /gene="nuoI"
                     /locus_tag="ACHOTW_000525"
     CDS             complement(8545..9087)
                     /gene="nuoI"
                     /locus_tag="ACHOTW_000525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP9842695.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(9102..10079)
                     /gene="nuoH"
                     /locus_tag="ACHOTW_000526"
     CDS             complement(9102..10079)
                     /gene="nuoH"
                     /locus_tag="ACHOTW_000526"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP9842696.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(10076..12802)
                     /gene="nuoG"
                     /locus_tag="ACHOTW_000527"
     CDS             complement(10076..12802)
                     /gene="nuoG"
                     /locus_tag="ACHOTW_000527"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP9842697.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12855..14192)
                     /gene="nuoF"
                     /locus_tag="ACHOTW_000528"
     CDS             complement(12855..14192)
                     /gene="nuoF"
                     /locus_tag="ACHOTW_000528"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP9842698.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(14189..14689)
                     /gene="nuoE"
                     /locus_tag="ACHOTW_000529"
     CDS             complement(14189..14689)
                     /gene="nuoE"
                     /locus_tag="ACHOTW_000529"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP9842699.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(14692..16494)
                     /gene="nuoC"
                     /locus_tag="ACHOTW_000530"
     CDS             complement(14692..16494)
                     /gene="nuoC"
                     /locus_tag="ACHOTW_000530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP9842700.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16588..17250)
                     /gene="nuoB"
                     /locus_tag="ACHOTW_000531"
     CDS             complement(16588..17250)
                     /gene="nuoB"
                     /locus_tag="ACHOTW_000531"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP9842701.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(17266..17709)
                     /gene="nuoA"
                     /locus_tag="ACHOTW_000532"
     CDS             complement(17266..17709)
                     /gene="nuoA"
                     /locus_tag="ACHOTW_000532"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP9842702.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(18340..19278)
                     /gene="lrhA"
                     /locus_tag="ACHOTW_000533"
     CDS             complement(18340..19278)
                     /gene="lrhA"
                     /locus_tag="ACHOTW_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP9842703.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
     gene            20198..21415
                     /gene="alaA"
                     /locus_tag="ACHOTW_000534"
     CDS             20198..21415
                     /gene="alaA"
                     /locus_tag="ACHOTW_000534"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP9842704.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            21499..22098
                     /gene="yfbR"
                     /locus_tag="ACHOTW_000535"
     CDS             21499..22098
                     /gene="yfbR"
                     /locus_tag="ACHOTW_000535"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9842705.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(22157..23989)
                     /gene="yfbS"
                     /locus_tag="ACHOTW_000536"
     CDS             complement(22157..23989)
                     /gene="yfbS"
                     /locus_tag="ACHOTW_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP9842706.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(24076..24726)
                     /gene="hxpA"
                     /locus_tag="ACHOTW_000537"
     CDS             complement(24076..24726)
                     /gene="hxpA"
                     /locus_tag="ACHOTW_000537"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP9842707.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(24737..25231)
                     /gene="yfbU"
                     /locus_tag="ACHOTW_000538"
     CDS             complement(24737..25231)
                     /gene="yfbU"
                     /locus_tag="ACHOTW_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9842708.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(25314..25769)
                     /gene="yfbV"
                     /locus_tag="ACHOTW_000539"
     CDS             complement(25314..25769)
                     /gene="yfbV"
                     /locus_tag="ACHOTW_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP9842709.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            26107..27309
                     /gene="ackA"
                     /locus_tag="ACHOTW_000540"
     CDS             26107..27309
                     /gene="ackA"
                     /locus_tag="ACHOTW_000540"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP9842710.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            27384..29528
                     /gene="pta"
                     /locus_tag="ACHOTW_000541"
     CDS             27384..29528
                     /gene="pta"
                     /locus_tag="ACHOTW_000541"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9842711.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29718..31238
                     /gene="yfcC"
                     /locus_tag="ACHOTW_000542"
     CDS             29718..31238
                     /gene="yfcC"
                     /locus_tag="ACHOTW_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9842712.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(31271..31813)
                     /gene="yfcD"
                     /locus_tag="ACHOTW_000543"
     CDS             complement(31271..31813)
                     /gene="yfcD"
                     /locus_tag="ACHOTW_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP9842713.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31871..32422)
                     /gene="yfcE"
                     /locus_tag="ACHOTW_000544"
     CDS             complement(31871..32422)
                     /gene="yfcE"
                     /locus_tag="ACHOTW_000544"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP9842714.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(32478..33122)
                     /gene="yfcF"
                     /locus_tag="ACHOTW_000545"
     CDS             complement(32478..33122)
                     /gene="yfcF"
                     /locus_tag="ACHOTW_000545"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP9842715.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            33258..33905
                     /gene="yfcG"
                     /locus_tag="ACHOTW_000546"
     CDS             33258..33905
                     /gene="yfcG"
                     /locus_tag="ACHOTW_000546"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP9842716.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33962..34324
                     /gene="folX"
                     /locus_tag="ACHOTW_000547"
     CDS             33962..34324
                     /gene="folX"
                     /locus_tag="ACHOTW_000547"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP9842717.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            34345..35238
                     /gene="yfcH"
                     /locus_tag="ACHOTW_000548"
     CDS             34345..35238
                     /gene="yfcH"
                     /locus_tag="ACHOTW_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP9842718.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(35286..36176)
                     /gene="rpnB"
                     /locus_tag="ACHOTW_000549"
     CDS             complement(35286..36176)
                     /gene="rpnB"
                     /locus_tag="ACHOTW_000549"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP9842719.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(36373..37146)
                     /gene="hisP"
                     /locus_tag="ACHOTW_000550"
     CDS             complement(36373..37146)
                     /gene="hisP"
                     /locus_tag="ACHOTW_000550"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP9842720.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(37154..37870)
                     /gene="hisM"
                     /locus_tag="ACHOTW_000551"
     CDS             complement(37154..37870)
                     /gene="hisM"
                     /locus_tag="ACHOTW_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9842721.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37867..38553)
                     /gene="hisQ"
                     /locus_tag="ACHOTW_000552"
     CDS             complement(37867..38553)
                     /gene="hisQ"
                     /locus_tag="ACHOTW_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP9842722.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38643..39425)
                     /gene="hisJ"
                     /locus_tag="ACHOTW_000553"
     CDS             complement(38643..39425)
                     /gene="hisJ"
                     /locus_tag="ACHOTW_000553"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP9842723.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39646..40428)
                     /gene="argT"
                     /locus_tag="ACHOTW_000554"
     CDS             complement(39646..40428)
                     /gene="argT"
                     /locus_tag="ACHOTW_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP9842724.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40694..41263)
                     /gene="ubiX"
                     /locus_tag="ACHOTW_000555"
     CDS             complement(40694..41263)
                     /gene="ubiX"
                     /locus_tag="ACHOTW_000555"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP9842725.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(41358..42875)
                     /gene="purF"
                     /locus_tag="ACHOTW_000556"
     CDS             complement(41358..42875)
                     /gene="purF"
                     /locus_tag="ACHOTW_000556"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9842726.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(42912..43400)
                     /gene="cvpA"
                     /locus_tag="ACHOTW_000557"
     CDS             complement(42912..43400)
                     /gene="cvpA"
                     /locus_tag="ACHOTW_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP9842727.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43841..44503)
                     /gene="dedD"
                     /locus_tag="ACHOTW_000558"
     CDS             complement(43841..44503)
                     /gene="dedD"
                     /locus_tag="ACHOTW_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP9842728.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(44493..45761)
                     /gene="folC"
                     /locus_tag="ACHOTW_000559"
     CDS             complement(44493..45761)
                     /gene="folC"
                     /locus_tag="ACHOTW_000559"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP9842729.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45831..46745)
                     /gene="accD"
                     /locus_tag="ACHOTW_000560"
     CDS             complement(45831..46745)
                     /gene="accD"
                     /locus_tag="ACHOTW_000560"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9842730.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46901..47560)
                     /gene="dedA"
                     /locus_tag="ACHOTW_000561"
     CDS             complement(46901..47560)
                     /gene="dedA"
                     /locus_tag="ACHOTW_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9842731.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(47643..48455)
                     /gene="truA"
                     /locus_tag="ACHOTW_000562"
     CDS             complement(47643..48455)
                     /gene="truA"
                     /locus_tag="ACHOTW_000562"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9842732.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(48455..49468)
                     /gene="usg"
                     /locus_tag="ACHOTW_000563"
     CDS             complement(48455..49468)
                     /gene="usg"
                     /locus_tag="ACHOTW_000563"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9842733.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(49534..50670)
                     /gene="pdxB"
                     /locus_tag="ACHOTW_000564"
     CDS             complement(49534..50670)
                     /gene="pdxB"
                     /locus_tag="ACHOTW_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP9842734.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50769..51764
                     /gene="flk"
                     /locus_tag="ACHOTW_000565"
     CDS             50769..51764
                     /gene="flk"
                     /locus_tag="ACHOTW_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP9842735.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(51761..52939)
                     /gene="yfcJ"
                     /locus_tag="ACHOTW_000566"
     CDS             complement(51761..52939)
                     /gene="yfcJ"
                     /locus_tag="ACHOTW_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9842736.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(53214..54434)
                     /gene="fabB"
                     /locus_tag="ACHOTW_000567"
     CDS             complement(53214..54434)
                     /gene="fabB"
                     /locus_tag="ACHOTW_000567"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP9842737.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            54593..56599
                     /gene="mnmC"
                     /locus_tag="ACHOTW_000568"
     CDS             54593..56599
                     /gene="mnmC"
                     /locus_tag="ACHOTW_000568"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP9842738.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(56720..56998)
                     /gene="yfcL"
                     /locus_tag="ACHOTW_000569"
     CDS             complement(56720..56998)
                     /gene="yfcL"
                     /locus_tag="ACHOTW_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP9842739.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(57032..57580)
                     /gene="epmC"
                     /locus_tag="ACHOTW_000570"
     CDS             complement(57032..57580)
                     /gene="epmC"
                     /locus_tag="ACHOTW_000570"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP9842740.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(57580..58389)
                     /gene="yfcA"
                     /locus_tag="ACHOTW_000571"
     CDS             complement(57580..58389)
                     /gene="yfcA"
                     /locus_tag="ACHOTW_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9842741.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(58389..59213)
                     /gene="mepA"
                     /locus_tag="ACHOTW_000572"
     CDS             complement(58389..59213)
                     /gene="mepA"
                     /locus_tag="ACHOTW_000572"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP9842742.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(59217..60302)
                     /gene="aroC"
                     /locus_tag="ACHOTW_000573"
     CDS             complement(59217..60302)
                     /gene="aroC"
                     /locus_tag="ACHOTW_000573"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9842743.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(60337..61269)
                     /gene="prmB"
                     /locus_tag="ACHOTW_000574"
     CDS             complement(60337..61269)
                     /gene="prmB"
                     /locus_tag="ACHOTW_000574"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9842744.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            61435..61986
                     /gene="smrB"
                     /locus_tag="ACHOTW_000575"
     CDS             61435..61986
                     /gene="smrB"
                     /locus_tag="ACHOTW_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP9842745.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(62157..62999)
                     /locus_tag="ACHOTW_000576"
     CDS             complement(62157..62999)
                     /locus_tag="ACHOTW_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP9842746.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(63001..63522)
                     /locus_tag="ACHOTW_000577"
     CDS             complement(63001..63522)
                     /locus_tag="ACHOTW_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9842747.1"
                     /translation="MKNNRAWALISGLILFSGTAPAADNLHFTGNLLGKSCTPVINGN
                     LLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKVTLMGTEDTDLPGFLS
                     IDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNFNAWLQTVNGRNVTSG
                     DFTATMTVTFEYF"
     gene            complement(63519..63989)
                     /locus_tag="ACHOTW_000578"
     CDS             complement(63519..63989)
                     /locus_tag="ACHOTW_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9842748.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVPYTITCDTAVRDPQMEMTLTWSGTQSDFDDSAVATDLNGLG
                     IHLKQAGSDFKLHTPLVVNETSLPVLTAVPVKKSGVDLPESDFEAWATLQVDYQ"
     gene            complement(63986..64486)
                     /locus_tag="ACHOTW_000579"
     CDS             complement(63986..64486)
                     /locus_tag="ACHOTW_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9842749.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            complement(64497..65255)
                     /locus_tag="ACHOTW_000580"
     CDS             complement(64497..65255)
                     /locus_tag="ACHOTW_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9842750.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            complement(65278..67917)
                     /locus_tag="ACHOTW_000581"
     CDS             complement(65278..67917)
                     /locus_tag="ACHOTW_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9842751.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQGTLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            complement(67999..68562)
                     /gene="yfcV"
                     /locus_tag="ACHOTW_000582"
     CDS             complement(67999..68562)
                     /gene="yfcV"
                     /locus_tag="ACHOTW_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9842752.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        17078 a        18543 c        17097 g        15844 t
ORIGIN      
        1 ccccctcaaa tcacatgatt cagataatct cctgttcaaa aaactattct ccccttcgaa
       61 attaatggca attatcggtg aggacattcc tctcatatca gaaaaacagt cactttcaaa
      121 ggtactttta aatgataaga ataatgaact gagcgatggg acaaacttct gggataaaaa
      181 tcgtcaatta accacagatg aaatagcttg ctatcttaaa aagatcgccg ccaatgcaaa
      241 aaatactcaa gtcaattatc ctactgattt ctacctcccc aattccaaca gcacttacct
      301 ggaagtcgct ctcaatgata atattaagag cgatccatca tggccgaaag aagtccagtt
      361 attccccata aatactggcg gacactggat attagtttcg ctacagaaaa tagttaatga
      421 aaaaaacaac acacaacaaa taaaatgcat catattcaat tcattacgtg cactaggcca
      481 tgaaaaagaa aattcactta agcgtatcat taacagtttc aatagtttca attgtgaccc
      541 cacgagagaa acgccgaata ataagaatat aacagatcat ttaactgaac cagagataat
      601 atttttacat gccgatcttc agcaatactt aagccaaagt tgcggtgcat ttgtgtgcat
      661 ggcagcccag gaagtgattg aacaaatgga aagcaattct gacagcgccc cctatacgtt
      721 attaaaaaac tatgctgaca gatttaaaaa atattcagca gaagagcagt acgaaattga
      781 ttttcaacat cgtctggaaa acagaaattg ttatttagat aaatatggcg atgcaaatat
      841 caatcattat tatagaaact tagaaataaa aaactcacac cccaaaaata gagcatcaag
      901 caaaagagta agttaacatc attgttaatt aattcgacca gtcagcaaat atgctgactg
      961 gtcgaacata gtttgctaaa aagtcatcac tgactgatgt cagtcacacc acccgccagt
     1021 tcaatcttcg ggtatttcca gcgtatctcg gcacgcgatt gctggtctgt tgatgtagtg
     1081 gattgttgtt gctgactttt cttaccctca gatttctgcc ccctgtcaac tcaatctgcc
     1141 aataaataac ataattatat ttttccttat atgaattcct ttttaaaata gcgaagccac
     1201 tgtaaaagat actttttgta tagatctaac aaccgtaata aaacaaacac tcataactca
     1261 ttataaataa tgataaaata acgtctttta ttgataattt acatatgaaa aaaattttcc
     1321 ttttgttata ttatttctgt tttcactccc tctgattatt ttttaccaac cctgggtcac
     1381 tgcattatcg ccaacgcccc gacacgtaat tcctgagcaa ttagaaaaaa cagttcgtta
     1441 tcttacacaa actgtgcatc cacgtaatgc cgacaatatt gataatctga ataggtccgt
     1501 agagtatata aaaaaaggtc tttgtcagta gcggtgccag agttaccgcg cagggcgtcc
     1561 ccaattggct attacgactc tgcgcctggt agccaggatt acccttaccc gcaatgtcct
     1621 ggctttatcc cgatcggggg gactttattg cctggtcggc agaatacagg atatcaacgc
     1681 cgttcgtcag gtaaaaacgg cattgttatc atcccgggat ttatctgttt attctatgaa
     1741 tgccccatgg tttattcacg gtattgattt ctctgaccac cttaattatt ggcaacacga
     1801 tattctcgcc gtaatgatta ctgacaccgc tttttatcgt aataaacaat accacttgcc
     1861 cggtgataca gcagacagat tgaattatca gaaaatggct cagatggtgg atggtgttat
     1921 aactttgtta cacaacagta aataataaaa atttctcgag gcgggtccag aaacagcgac
     1981 atgtacattt tattttccct cgtctgtttt aagatgcgaa acataaaatt caatggagga
     2041 aaaataaatg ggaatgattg gttattttgc agaaattgac tcagaaaaga ttaaccaact
     2101 gcttgagtct actgaaaaac ctttgatgga taatatacat gacacgcttt caggattacg
     2161 ccgactggat attgataaac gctgggattt tttacacttt gggctgacag gcacctctgc
     2221 gttcgatcca gcgaagaatg atccgctgag tcgtgccgtg cttggtgaac acagcctcga
     2281 agatggtata gatggtttcc tgggattgac atggaatcag gagttagccg cgacaatcga
     2341 ccgactggaa tcactggatc gaagtgagct gcgtaaaaag ttttccatta agcgactcaa
     2401 tgaaatggag atttatccgg gagtgacctt ttcagaagag ttagaggggc aactctttgc
     2461 ttcgatcatg cttgatatgg aaaagctgat tagcgcgtat cgacgtatgc tgcgacaagg
     2521 gaatcatgcg ctgacggtta ttgttgggtg atggtgtcag ggcaccaaaa aaaaccgccg
     2581 aacatgtcgg cggctttctg acttacaaac tgatagctta catcagcggc attgctaacc
     2641 gcacaatact aatcagcggt tgtggccata cacccagcac cagtaccaac agtgcagaaa
     2701 tcagcaccac gataccgccc gcgctgtact gccagtttga cggtgcatcg cgacccggtt
     2761 gttccggtgc gtgcaggtac aggctcaccg ccacgcgcag gtagtagtag aggccgattg
     2821 ccgaaccgac aaccacggca cccaccagcc accacaggtg tgcctggaca ccaaccgcca
     2881 gcacgtagaa cttaccgata aagcccagcg tcatcgggat accggccaga gacagcatca
     2941 tcaccgtcat caccgccgcg aggatcggac gatgccagaa cagaccgcgg taagagaaca
     3001 gggaatcagc atccgggcca cgatacgggc tggacatcag gctgaccacg ccgaacgcgc
     3061 cgaggctgct gaacagataa ccggccaggt aaacccctac cgcttccatc gacatctcgc
     3121 cagtttgcag cgcaatcagc gccaccagca gatagccgag gtgagagata gatgagtaac
     3181 cgagcagacg tttgatattg gtctggctca gcgccatcag gttaccgaag atgatggagg
     3241 caaaggcgat aatcgccagc accacccgaa tcgcttcgct gtcacccacc ggtgcgtaga
     3301 ggaacagacg catcaccaca ccgaagatag cgattttgct cgccgtcgcc aggaaagtgg
     3361 aaaccggcgc aggcgcgccc tggtatacgt ctggcgtcca caggtggaac ggcaccagag
     3421 agagtttgaa gccgaggcca acaatcatca ggccgaaacc tgccagcaac agcggctcgt
     3481 ttagcatacc gtcgccgagg tttttaccca gcgcgacaaa cgacaggtcg ccagactgcg
     3541 catacaccag cgccataccg aacagcagga aagaagacgc tgcggcagaa aggatggtgt
     3601 atttgatact ggcttccagc gaacgtttct ggcggaaagc gtaaccgacc aggccaaaca
     3661 gcggcaaaga gatcagttca ataccgagga acagagacgc cagatggttt gcattcgcca
     3721 gcaggatccc gcccagcgcg gcaattaaca ccaacaggta gaactcatcc ttgttgtcgt
     3781 tatagccttc aagccacggg taggcgaaag tacaggtggc gaggctcgcc aacaatacca
     3841 gcccggtgta aagcatggcg aaaccatcaa cgcgcatcag cggcgtaacg tccatagcgc
     3901 ccgcctggcc aacaaaccag agcgaaacca gcgccgcgtt aagcccaata accgagagcg
     3961 tagcgttgag gaaatgattg cgtcgccacg caatggagag catcacaacc accaccgtca
     4021 agccgacgat cagcaacggt agcagtgcga tcaggttttg tggagttatt gtcatggcga
     4081 tttacggcct tgtagtagta acggaattaa caaaccactg ctggatattg ccaatcgcgg
     4141 agtgcgaggt atccagaatc ggctgcggat agaagcccag cagtaccagc agcaccacca
     4201 gcaacaggat cataaacagc tcacgcagcg acatccctgg cagttcctgg ctggcaatct
     4261 ggcttttcgc tttaccgaag taagcgcgat gtaacatcgc cagcgaataa acagatgcaa
     4321 agaccagccc aaaggtagag ataacggtaa tcaccgggac aacctggaag ctgccgaaca
     4381 gaatcataaa ttcgccgacg aagttaccgg tgcctggcat cccgagcgtt gccaccgcaa
     4441 agaacagtga caaggctggc agccatttca tcttgctcca cagaccgccc atcatgcgca
     4501 tgtcgcgggt atggatacgt tcataaagct gaccacaaag aataaacaga cccgccgctg
     4561 acaagccgtg cgcaatcatc tggattaccg cgccctggta ggccaactgg ctgccggtgt
     4621 agatagcaat cagtacgaag cccatgtggg aaaccgaggt gtaggcgatc agacgtttga
     4681 tatcggtctg ggcgaaggcc atccacgcac cgtagaagat gccgataaca cccagccaca
     4741 tggcgattgg cgcgaactct gccgacgcgt ttgggaacag cggtagggag aaacgcagca
     4801 gaccgtaagc ggcggttttc agcaagatcc ccgcgaggtc cacggaaccg gcggtcggtg
     4861 cctgtgagtg cgcatccggc agccagccat gcagcggaac caccggcatt ttcaccgcaa
     4921 acgcgatgaa gaagcccagc atcaacagat attccacacc actggacatt ggcgtattca
     4981 gcagctcttc atagttgaag gtccagacgc cggtcgcatt gtagtgaaca aaaaccagcg
     5041 ccaggatggc gatcaacatc accagaccac tcgcctgggt gtaaatgaag aacttggttg
     5101 ccgccgtgat acgcgtttta ccatcagagg ctttatgccc ccacagtgcg atcaggaagt
     5161 acatcggcac cagcatcatt tcccagaaga agaagaacag gaacatgtcg atggcaagga
     5221 acacgccgat aacgccgccc aggatccaca tcaggttgag gtggaagaag ccctgatatt
     5281 tttcgatctc tttccacgaa cagagtaccg ccagcacacc gagcagaccg gtcagcacga
     5341 ccatcagcag cgacagcccg tcaatggcga gatggataga gataccaaaa cgcgggatcc
     5401 acggcatgtc gaattcagac tgccactgcg gaattccggc ggattgcgtc agtgaataac
     5461 cgccctgcaa ccacagttgc agcgacagcg ccagcgtcaa tcccatggtg atcagcgcga
     5521 tccagcgcgg caccttgacg ccaaagcgtt cggtctgcca gcacaggaag ccgccgataa
     5581 aggggattag tattaaccag ggtaataaca tagctaagta tgtcccttat atttactcct
     5641 gctcaggcta tctcactcgc cagcccgaat cccggcagtg ctcgcaatcc tcacgtactt
     5701 aagtacgctc cggttgctgt gcgctggcgg tatccggtct gacttcgccg atgacgcctg
     5761 tacttcagga acagttacct aaaatttatt cttagttcac agacccggcg caacgccggg
     5821 tatattcact cagccaacaa ctcaacgcag taccatcaac agtgccagca ccacgaccgc
     5881 accgatgctc atggatgcca cataccagcg cagataaccg ttctcgctta acagcaggcc
     5941 tttacctgca aagcgggaaa ggacagccgg gatgttcatc attgagttca gcggatcgcg
     6001 tttcagcaac caggcaatac ccaggaacgg cttgacgaac actttgtcgt acagccagtc
     6061 aaagccccag gcgttgtacc accaggtgcc cagcagacgg cccggcgcac tgttggcgat
     6121 ggaggtcacc agagtacgtt tacccagcca cagccaggct gccagcagaa tgccgaccac
     6181 cgcgaccacg ccagaggtaa tttccagggt caacatgctg ccgtgcgcca gttccgtcgt
     6241 ttgcggaagc acgccctgca gcggcggtac aatcagtgcg ccaacgaagg tggaaaggat
     6301 cagcagcaca atcagcggca ggctgtgagt tacccctttc acggcgtgag cgtgaatttg
     6361 ttcttttccg tggaagacga tgaaaatcat acggaaggtg tagagcgagg tcataaacgc
     6421 accgaccaga cctgccacca tcagattgat atgaccattc gccatcgcac ccgcgaggat
     6481 ctcatcctta ctgaagaagc ccgcagtgac tagcggcagt gccgacagtg ctgcgccgcc
     6541 caccaggaag cagagataaa ccagcggaat agatttacgc agaccgccca tcttgaagat
     6601 gttctgttcg tgatggcagg cgagaatgac ggaaccggat gccaggaaca gcagcgcttt
     6661 aaagaacgcg tgggtcatca agtgaaaaat cgccgcatcc catgcctgca cgccaagcgc
     6721 gaggaacatg tagccaatct ggctcatggt agagtaagcg agaacacgtt tgatgtcggt
     6781 ctgtaccagc gcggcaaaac cggccagcag cagcgtaacc gccccaacaa tgcccaccag
     6841 atgcagaact tccggcgtca tcaggaacag gccgtgggta cgggcgatca ggtagacacc
     6901 cgcggttacc atcgttgcgg cgtggatcag cgcggagaca ggcgtcgggc ccgccatcgc
     6961 gtcggcaagc catgtctgca acggcaactg cgcagattta ccgaccgcac cgcccagcag
     7021 catcagcgtc gcccacatca gcatgttatt gccgtcagca aagtgcgctg gtgccagttc
     7081 caccatttcg cggaagttca gggtgcccag ttcgttgtaa agaatgaaca gcgcgaaagc
     7141 gaggaacacg tcacccacac gggtcacgac gaacgctttc attgccgctg cgccattctt
     7201 cggatcggtg taatagaacc cgatcagcag ataggagcac aggcccacgc cttcccagcc
     7261 gaggtacatc agcagcaggt tgtcggcaag caccagaacc accatgctgg cgataaacag
     7321 gttggtgtaa gcgaagaagc gagagtagcc ctcttcaccg cgcatatacc aggaggcgta
     7381 catgtgaata aggaaaccca caccagtgac caccgagagc atggtcagcg acaggccgtc
     7441 cagcaccagg ttaaaaccga tgttaaagtc gcctaccgac atccacgtcc acagcggctg
     7501 gctgtatgcc tgctcgccgt tagcgaagaa gtcaacgcca ataaaggcag ttaccagcgc
     7561 cgccaggccc acagagccaa cgccgacgat cgccgagacg ttttcagacc agcgcccacg
     7621 ggagaacgcc agcaggacga agccaatcaa tggcaaaata atggttaagg caagcatgtt
     7681 catccgcgca tctcacttac tgaatcgatg ttcaggttct ggcgacgacg gtgaagttgc
     7741 agcagcagcg caaggccgat actcgcttct gccgccgcga ggctgatggc gagaatgtac
     7801 atcacctgac cgtcggtctg gccccagtaa cttccggcga ccacaaaggc cagcgcggaa
     7861 gcgttaatca tgatttccag accaatcagc ataaacagca gattgcgacg gataaccaga
     7921 ccggttaagc caagaacgaa taagattgcc gcgaggatca gtccatgttg taaggggatc
     7981 atgcgtgctc ctccgttttt cttttcgcgc tgtcgtcttt acgattgctc agcacttcac
     8041 ccgcacgctc ttcacgaccg acgtggaagg ccacaaccag acccgcgagc agcagcatag
     8101 aagccagttc caccgccagt acgtaaggcc cgaacagcgt aatacccact gctttagcac
     8161 tgattggcgt accgtcgata ccttgatcgt taacaccgag aatggcgtaa acaatcacca
     8221 ccaacatgat ggctgacaaa attgccggac caatccacac ctgcggtttc agccactggc
     8281 gttcttgttc gatttctgaa ccgcccaggt tgagcatcat caccacgaac acgaacagca
     8341 ccataatggc acccgcgtag acgataattt ccagcgcacc ggcgaagtaa gcgcctagtg
     8401 agaagaacac cccggaaatc gccagcagcg aaataatcag gtacagcagt gcgtgtaccg
     8461 gattggtatg ggtgatcact cgcaaggtcg caagtatggc tatcaggcca cagatataaa
     8521 aagcgaactc cattgcccct ctccttacgg taacaggctc ttgacgtcga taggcttggc
     8581 ttcgttctct gcttcgccct tatctttgcc gtcgattgcc atacctgcca tccggtagaa
     8641 gttatactcc gggtatttgc ccggaccgga gatcagcaga tcctctttct cgtaaaccag
     8701 atcctggcgc ttgtattccc ccatttcgaa atccggggtt aactgaatcg ccgtggtcgg
     8761 acaggcttct tcgcacaaac cacagaaaat gcagcgtgag aagttgatgc ggaaaaattc
     8821 cggataccag cgaccgtctt tggtttctgc tttttgcagc gagatacagc cgaccgggca
     8881 ggctaccgcg cagaggttac aggctacgca acgctcttcg ccgtccgggt cgcgggtcag
     8941 aacgatacga ccacgataac ggggcggcag atagaccggc tcttccgggt acattcgcgt
     9001 ttcgcgtttg gcgaacgcgt gcaggccgat catccagata ctacgaacct gggtgccgaa
     9061 acctactaac aattctttta aggtcatggt cttattgccc cttattgcgc ctgccagaga
     9121 atgacagccg ccgttaccag caagttgatc agcgtcagcg gcaggcagat tttccagccg
     9181 aaggacatta cctggtcata acgcggacgc ggtaacgacg cacgaatcaa aatgaacatc
     9241 atcataaaga acgcggtttt cagcgcgaac cagatgaatg gcggtaacaa cgggccttgc
     9301 cagccaccga agaacagcgt caccatcaat gcagagatgg tcacaatccc gatgtactca
     9361 cccacgaaga acagaccgaa cttcataccg gaatattcaa tgtggtaacc atccgccagt
     9421 tcctgctcgg cttccggctg gtcaaacggg tgacggtgac acaccgccac gcccgcgatg
     9481 gcaaaggtaa taaaaccaaa gaattgcggg atgacgttcc acacatgcgc ctggctgttg
     9541 acgatgtcgg tcatgttgaa tgaaccggcc tgcgccacca cgcccatcag ggaaagcccg
     9601 aggaacactt cgtagctcag ggtctgcgca gaagcacgca tcgcaccgag cagcgagtat
     9661 ttgttgttac ttgaccagcc cgcaaacagc accgcgtaaa ccgccagacc ggccatcatc
     9721 aggaagaaca aaatcccgat gttcaggtcg gcaaccaccc aacccggact gactggcaca
     9781 atcgcaaagg ccagcagcag cgaggtaaag gcaatcatcg gtgccagggt aaagatgacg
     9841 cgatccgaga atttcgggat ccagtcttct ttaaagaaca ttttgatcat gtccgcaacc
     9901 agctggagcg aaccacccca gccaacacgg ttaggtccgt aacggttctg gaacagaccc
     9961 agcaggcgac gttcgccaaa gctcatgaat gccccgcagg tgacaaccac cagcaggatc
    10021 accaccgctt tgaggatggt cagcaggatc tcaatcagtt ccggtgatat ccaactcatt
    10081 gttgtgcctc cttgagatcc tcaagatgcg cgccagccag caccggagca atgccggaca
    10141 tacccatcgg caagcccacc tgccctgccg tcagcccttc agcgatttca accggcagag
    10201 tgaccgtgtt gccatcgtaa ctaaaggaga cgcgtgtacc tgcgtttaca cccaacttcg
    10261 cggcatccgc tgggttgagt ttgatgtacg gctgcggcat acgacactgg aagaccggag
    10321 cacgctgtga caattcatcg ctgccaaaca ggtggtagta cggcgcgata cgccatttcc
    10381 cgtcctgcgg ctggaagcgt gccggtacgc tggtgaagta atccagacca ttttcgctgg
    10441 tttcaaacag acgcacaccc ggatcgccaa agcgcagttt gccgcccact tcgtcctgga
    10501 atttgttcca cgcctgcggg gagttccagc ccggagccca ggcaaacggc acttgcgaac
    10561 ggtgcgcggt cggctggttg ttaccttcca tcgagaaggt gaacatggtg tcaatatcct
    10621 gcggctgacg cggctcatga acgctgatat tggcgcgcat ggcggtacga ccgctgtaac
    10681 ggtgcggttc acgggccagt ttctgcccac gaatacggaa tgtcgcatct ggcgcagcat
    10741 ctttgatacc cgccagttcc gggattttcg ccacaacagc gtcaattaca tggtcgagct
    10801 gcgtccagtc cacttcacgg ctcagcaagg tgctgtgcag cgagtgcagc cagcgccaac
    10861 tttccagcat gacagttttg ctgtcgtaat aagccggatc gtaaacctgg aagaaacgtt
    10921 gggcgcgacc ttcattatta attaccgtac cgtcgctttc agcaaagctg gcagcggaaa
    10981 gcaccagatg ggcgttttcc atgatcgctg tgcgttgatg gtcaaccacc atcaccagcg
    11041 gcgctttagc cagcgcagca ttcacgcggg tagcagaagc gtgacgatgc agatcgtttt
    11101 ccagcaccac caccgcgtcg gcgcgtccgg tttccagttc ggttaacgct tcttcaagcg
    11161 atccaccgcc cataatgccc aaccccatgc tgttgacgga acgggcaatc atggtgatac
    11221 cgacgtcagc gccgcgacct ttcagggctt tcgcgacgtt agccgcagcc tgaatcacct
    11281 ctaagctacc ggcgttcgtc ccggagataa tcaacggttt cttcgcaccc gccagtgcct
    11341 gcacgatgac gtcgattttg ctttgcagct caggttcaat accgtcaacc gctggcgcag
    11401 agttatccag cgcatgggcg atggcaaaac ctaaacgcgc ctgatcttca accggtgcgc
    11461 ggtaagtcca cgccgcgata tcatccagac gggtgtcatc aacgttggta acaaacagcg
    11521 gatgcttagc acgttgaccg atattgagga ttgccgcaat ctgccagtca gccactttct
    11581 gtgctgccgc catttcgcgc gctttacctt tcaccgcctg gcgcactgcc agcgcgacgc
    11641 gcgcgccggt ctgggtaacg tcttcgccca gcaccagtac cgcatcgtaa gattcgattt
    11701 cgcgcagagc cggagtataa atgccgcctt cacgcagcac tttcagcgcc agttgcagac
    11761 gttcctgctc accgtgagcg ataccggtgt agaagttttc ttcgcccacc agttcacgca
    11821 gcgcaaagtt gctttccacg ctggcacgcg gagaaccaat accgatcact ttcttcgact
    11881 gacgcagaat atctgccgcg ccctgcattg cctgttcggc gttgagggta atgaaatcat
    11941 cgccacgacg ctgtactggc tgacgcggac gatctttcag gttgacgtaa ccgtaaccga
    12001 aacgaccacg gtcgcagagg aagtagtggt ttaccgtacc gttgtaacgg ttttcgatac
    12061 gacgcagttc gccataacgc tcgccggggc tgatgttaca gccgatggaa cattgctggc
    12121 agatgctcgg cgcaaactgc atatcccatt tacggttgta acgctcggag tgcgttttgt
    12181 cggtaaatac gccggtcggg caaatttcga ccaggttacc ggaaaattcg ctttccagcg
    12241 tgccgtcttc cgggcgaccg aagtagacgt tgtcatgcgc gccataaacg cccagatctg
    12301 taccgtcagc gtaatctttg tagtaacgca cgcagcggta gcaggcgatg cagcggttca
    12361 tttcgtgaga gatgaacggc cccaaatcct gattacggtg ggtacgtttg gtgaaacggt
    12421 agcgacggaa gctgtgtccg gtcatcacgg tcatatcctg aagatggcag ttaccgcctt
    12481 cttcacatac cggacagtcg tgcgggtggt tggtcattaa ccactcgacc acactttcac
    12541 ggaactgttt cgcttcttcg tcgtcaatgg aaataaaggt gccatcggaa gccggtgtca
    12601 tacaggacat caccaggcga ccacgcgtgt cttccgcgtt ttggtattgc ttcaccgcac
    12661 actggcggca agcaccgacg cttcccagcg ccggatgcca gcaaaagtaa ggaatatcaa
    12721 ggcccagaga cagacaagct tccagcaggt tgtccgctcc gttgacctcg tattctttgc
    12781 cgtctacatg aattgtagcc attagcatgc ttccagtttt ctcagtcaga gactgagcgt
    12841 taatcgaaat tcggttacca gcgctctttc agcaggttcg gctgaatccc attaatcaaa
    12901 tgggtattgc tgaacggctg tttgatcccc gcctcaaatt cttcgcggaa atatttgatg
    12961 gcgctctgta acggctccac agcaccaggt gcgtgggcac agaaagtttt acccgggcct
    13021 aagaatcgac acagttgctc aagtgtttcg atatcgcccg gctgaccttc gccacgctcc
    13081 agcgcacgca gaattttcac gctccacggc agaccgtcgc ggcacggcgt acaccagccg
    13141 caggactcac gggcgaaaaa ctcttccagg ttacgcacca gcgacaccat gttgatttca
    13201 tggtcaaccg ccatcgccag cgccgtaccc agacggctgc ccgctttacc gatactttcg
    13261 aattccatcg gcaggtcaag gtgcgcttcg gtcaggaagt cggtgcccgc gccgcctggc
    13321 tgccaggctt tgaatttcag accgtcacgc ataccaccgg cgtaatcttc gaggatctcg
    13381 cgtgcggtgg tgccgaacgg cagttcccac agtcccggat ttttcacccg accggagaag
    13441 cccatcagct tggtgccagc atctttactt ttcgagatgt tctgatacca ctccacgccg
    13501 ttagcgagga tcgccggaac gttacacagg gtttcgacgt tgttgacaca ggtcggtttg
    13561 ccccatacgc cggaggttgc cgggaagggg ggtttcgagc gtgggttagc acgacgccct
    13621 tccagggagt tgattaacgc tgtttcttcc ccgcagatgt agcgccctgc cccggtatgg
    13681 acgaacagtt cgaagtcgaa acctgttccc ataatgtttt tgccaagcaa tcccgcttca
    13741 gtcgcttcgg caatggcgcg gcgcagattt actgccgctt caatatattc gccacgcagg
    13801 aagatgtagc cacggtaagc tttcagcgca aacgcggaga tgagcatacc ttccaccagc
    13861 aggtgcggca gttgctccat caacaggcgg tctttatagg tgcccggctc catttcatcg
    13921 gcgttacaca gcaggtaacg gatgttcatg gattcgtctt tcggcatcag gctccacttc
    13981 aagccagtcg aaaagcccgc gccgccgcgc cctttcagac cagcgtcttt tacctgatta
    14041 acgatttcgt ccggagacag cccggtcagc gccttacgcg ccccttcgta accgtttttg
    14101 ctgcggtatt cgtccagcca cactggctgt ttgtcatcgc gcagacgcca ggtcagcgga
    14161 tgcgtttcgg gagtacggat aatgtttttc atttataccg ctccagcagt tcagggatcg
    14221 cttccggggt cagattcgcg tgagtgtcct catcgatcat catgtttggc cctttatcac
    14281 agttccccag gcagcaagtt ggcagcagcg taaagcggcc atcaaatgtc gtttgccctg
    14341 gtttgatgtt cagctttttc tcgagcgccg cctgaatacc ctgataccca ttgatatgac
    14401 agaccacgct gtcgcaataa cggatcacat ggcggccaac cggctggcgg aagatctggc
    14461 tgtagaacgt tgccacgcct tcgacgtcgc ttgccggaat acccagcaca tcggcgatcg
    14521 cgtggatcgc accatccggc acccagccac gctgcttctg aacgattttc agcgcttcaa
    14581 tggacgccgc acgcgggtct tcgtagtggt gcatctcgtg ctcgatcgct tcacgctctg
    14641 ccgcactcag ctcaaaagcc tcggtttgtg gttgttgatt ctcgtgcata attagcggtc
    14701 cacatctgac ataacaaaat cgatactgcc cagataaaca atcaggtcag acaccaggct
    14761 gccgcggatc gccgccggaa tttgctgcaa atgcgcataa ctcggcgtgc ggatacgagt
    14821 gcgataactc atggtgctac cgtcgctggt caggtagtaa ctgttgatcc ctttggtcgc
    14881 ctcaatcatc tggaaggatt cattggcagg catcaccggc ccccacgaca cttgcaggaa
    14941 gtgggtgatc agggtttcga tatgttgcag cgtgcgctct ttcggcggcg gcgtggtcag
    15001 cgggtgatcc gctttgaacg ggccttccgg catgttgttg aggcactgct caagaatgcg
    15061 cagactctgg cgcagctctt ccactttaag cattacgcgg gtgtagcagt cagaaacacc
    15121 accaccaacc gggatttcaa agtcgaagtt ttcatagcca gaataaggac gcgccttacg
    15181 cacgtcgaag tcgatcccgg tagcacgcag gcccgcgcca gtggtgcccc actccagcgc
    15241 ctctttcgcg ccataggcgg caacgccctg ggaacgacct ttcagaatgg tattttgcag
    15301 cgccgctttc tcgtaagacg ccagacgttt cggcatccag tcgaggaact cacgcagcag
    15361 gcgatcccag ccgcgcggca ggtcgtgcgc tacgccgcca atacggaacc acgccgggtg
    15421 catacggaaa cccgtgatcg cttccaccag atcgtaaatt ttctgacgat cggtaaaggc
    15481 gaagaacacg ggcgtcattg cgccgacgtc ctgaataaag gtcgagatat acagcaggtg
    15541 gctgttgatg cggaacagtt cggagagcat tacgcgaatg acgttaacgc gagccggcac
    15601 ggtgatcccg gccagtttct ctaccgccag cacgtaaggc atttcgttaa cgcagccgcc
    15661 gaggtattcg atacggtcgg tatacggaat gtagctgtgc caggactggc gttcgcccat
    15721 tttctccgca ccacggtggt ggtagccgat gtctggtacg cagtcgacaa tctcttcgcc
    15781 gtcgagctgc aaaacgatac ggaaagcacc gtgcgccgac gggtggttcg gaccgaggtt
    15841 gaggaacatg aagtcctcgt tttcggtgcc gcgcttcatc ccccactctt ccggtttgaa
    15901 ggtcagggct tccatctcca gatcctgttt ggctttggtc agctcaaacg gcgagaattc
    15961 ggtagcgcgc gccgggtaat ctttacgcag cgggtgacct ttccaggttt gcggcatcat
    16021 gatgcggcgc aggttcgggt gaccgtcgaa tgtaatgcca aacagatccc aggtttcacg
    16081 ctcataccag ttagcgttcg ggaacagttt ggtgaaggtc ggtacgtgca ggtcgttttc
    16141 tgccagcgcc accttcagca tgatgtcgcg gttacgatcg atagaaatca gatggtagaa
    16201 aacggaaaaa tccgcggcag gtaacccttc gcggtgcgtg cgcagacgtt cgtccatgcc
    16261 gtgtaagtca aacagcatga cgtaaggttt cggcagtttc tttaagaaat cgccaacttc
    16321 cagtaattgt tcacgcttga tccacacaac gggaaccccg gtgcgagtcg cctgaacagt
    16381 aaaggcatcc ggcccaaaac ggttgcgcag ttcgccaatt accggatcat caagatgatc
    16441 gcgggtctgc caggcgggtt cttgcgcggt taagtcggtc atattgttca ccattgcaaa
    16501 tggtccgtgg tgactgtcgg cgcagcaagc ttcgcgaaat agatttagga ataagcgaag
    16561 aaaaatccct ttgccgacag gcgcaaatta aatctcgtca ggtgtacgca ggttggttac
    16621 ggcaatgcgt tcaccgcgct tgcgttcgcg ctctgattgc atattggcgc gataaacgcc
    16681 ctgatcgcca accacccagg agagcggacg acgttctttg ccgatagatt cctgcaacag
    16741 catcagtgcc tgcatgtacg cttcaggacg cggcgggcag cccgggatat acacatcaac
    16801 cgggatgaat ttatcgacgc cctgcacaac ggaataaata tcgtacatac caccagagtt
    16861 ggcacaggca cccattgaga taacccattt tggttccagc atctggtcat acagacgctg
    16921 aataaccggt gccattttgg taaagcaggt tcctgcaacc accatcaggt cagcctgacg
    16981 cggcgaagca cgcaatactt ctgcgccaaa acgcgccacg tcatgcaccg cggtaaacga
    17041 agtcaccatc tcaacgtaac agcaggaaag accgaagtta tacggccaaa ttgagttttt
    17101 acgaccccag ttaaccatgt cattgagctt gcccataaac acgtttttgt taacttcttg
    17161 ctccagaggg tcggttacga tctcctgctt ttgcaggggg taacggtcgt tctcaccgtt
    17221 gggatctatg cgggtgagcg tataatccat cttaatgcct cgcggttagc gttgacgatt
    17281 agcgatactg ttcgtttccg ggttcatacg ctcgcggcgt gaacgcgcgg gcgtccagtc
    17341 cagcgcgcca atacgcacca gataaaccag acctgccagt aacacaaaaa taaaaattgc
    17401 agcttccaca aagcctaccc agccgctttc gcggatagag gttgaccatg cgaacagata
    17461 cagcgcttca acgtcgaaga taacgaagaa catggccacc agataaaact tggcggacag
    17521 gcgtaagcgg gcggagccga ccgagtcgat accggattca aacggcacgt ttttcgacct
    17581 cgcgcgtgcg cgaccgccta aaaaccaacc gcctaccagc atcaggcaac ataggccaat
    17641 ggcaacgata agaaagatag cgaatgccca gtgatgagcg atgacttcag tggatgttga
    17701 catactcatt gcttactcat caaaagtagc gccagattca ctgctcttca cggcagatgg
    17761 acgccacatc gattcatggg gaggaataaa aaaaacctta caatcactgt agaaattctt
    17821 ttatacagct aattgatgtg gtcttttact cctttctata accttttgtc aactttaaca
    17881 aaagtttctt cacattagtt tacataatat caacaccatt agcatttaat gccctttcac
    17941 cccagatcct tgacgactcc aggataatta gatgttgttg aatcgtgtcc gttgtgaagc
    18001 aatggaaaaa atacgggtct attttgacag gaatttgtgt cgattcctcc ccccaaaaga
    18061 gagtattttc ttgatctgtg acacgctttt gtcattccat aacaaaaacg cagcaacaaa
    18121 tttacgtatt ttttaacatc attgtagcag gtgatttttt tcaggcgatt atttgtgcgt
    18181 acggggcgtg aatctctggt gattgaaaaa tgaacagttt tgtacgttct gcacgatgtg
    18241 aagaaggttc tttactaaaa aaaagccgct ggggtttaaa acacccccag cggctcgttt
    18301 tttacactat tgtctcagga attatctatc gtccgtcgat tactcgatat ccctttcaat
    18361 caacaatgaa tcatcccctt ccggagcaga tattgggctg tattgccacg gattatggta
    18421 gctctccatc gcctgataga tcacctgggc cagctcatta ttactggacg ggtcatagca
    18481 cagcaaatat tcggtatcag gtagcggcgg taagccatct actccactca gcacacgcaa
    18541 gtccggactc atcatctcga ccggccgtgc cgtcacgcca agaccggctt tcactgccgc
    18601 acgaacggcc ggaagcgtcg aggcgacata agccagtcgc cagggaatat ctgctttatt
    18661 aagcgtcgcc agcaccatat cgcgaaacgg acttggatca tccagcaata caagagggat
    18721 cggctcacct ttttgcaata cgtattccgc tgcgcagtac cagtgtgttg gcgaggtacg
    18781 caggttcaat gccttaaacg ctgaaggacg atgggtggtt accatcaaat ccacttcctg
    18841 ggattccagc atttctgcca tataggcatt acgtttaacg cggacatcca gcgctaattt
    18901 cggataaacc gaactcacgc gatttaacag gaaaggtaag atcgtatcgg cagattcatc
    18961 tgaagcaccg atagttaaca cgccctgaag attactgaac attaatgatg agcaggcctc
    19021 atcattaaaa cgcaggattt tcctggcgta accaagaagt tgaatgccat gttcagttaa
    19081 cagtttgttg cgaccgtgac gagcgaacag ttctttccca acgagttgtt ccagacgctg
    19141 catttgctga cttacggcgg actgagtacg acacacagcg gcagctgcgg cagcaaaagt
    19201 gttcagatcg gcaacagcaa caaatgttct cagcagatcg aggtcgaggt taattatcgg
    19261 acgatttgca cttatcatat attatcactt actggcggct catactgagc tggttaatgc
    19321 tgtgcacaca caaacaagca attccatttg taatgtgcct ccctggcagt ttcatccgga
    19381 acccggacga aagtaaaaat gcatatgagt tgcactaaaa aagcgactca cattgttccg
    19441 ttataatgcc tgaagtagat cacagaatat atcttcaggg atcgcatatc tattaagtta
    19501 ctcactcttt tctatttatg acatgcgcgt gtttgtataa atgtaaatgt gagtccttgt
    19561 tccactctcg tgcagcatcg ctggtcatac gcgaacacgt acaaacagca atggtgaggc
    19621 ggcatcaaga gcagggatcc gtcatttatg cgagcatttt accccaaaaa ccttttattt
    19681 ataagggtca ttgcgaatta tctgatgcaa agttatgtta tgttaggcaa agtaatcttc
    19741 tattattaat aagcacatca aaaccttttt gaatattaaa taataattaa ttagcatcat
    19801 cctcattcat taattccgct taacaatagt ttcacacctt gcccccattg cgacacctcg
    19861 gaaatcatca cgctgttaaa ctctccgtaa tgcggaaata tcatcatcgc gtttcattag
    19921 tgaattctta aatgaggcat tttcacacaa ttatcttaca gataaaaaaa ccagacttac
    19981 aattaagaat cagaacaatt cactatataa cattgcatgt aaagcatatg cacctcatta
    20041 ttttgtcatt attaagttat taacagcaca atcgagcctt cccctctggc aaaatcttat
    20101 tctgcagacc ttcaaaacac cgtcctgggg gagtacattg ttctaagctg acttccacgg
    20161 cagggagtgg cgataacagc aaaaaaggtc aagattcatg tcccccattg aaaaatccag
    20221 caaattagag aatgtctgtt atgacatccg tggtccggtg ctgaaagaag caaaacgcct
    20281 ggaagaagaa ggtaacaagg tactgaaact gaacatcggc aacccagccc cgttcggttt
    20341 tgacgcgcca gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta
    20401 ttgcgattcc aaaggtcttt actccgcgcg taaagccatc atgcagcact atcaggctcg
    20461 tggcatgcgt gatgttaccg tggaagatat ttacatcggc aatggtgtat cggagcttat
    20521 cgttcaggca atgcaggcat tgctgaacag cggggacgaa atgttggttc ctgcaccaga
    20581 ttacccacta tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc attatctttg
    20641 cgatgaatcc tctgactggt tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg
    20701 tacgcgtggg atcgttatta tcaacccaaa taacccaacc ggcgcggtat attccaaaga
    20761 gcttttaatg gagattgtgg agattgcacg tcagcataat ctcattatct tcgccgatga
    20821 aatttatgac aaaattctct acgacgacgc tgagcatcac tcaattgcgc cgctggcacc
    20881 tgacctgctg accattacct ttaatggact gtcgaaaacg taccgcgttg caggcttccg
    20941 tcaggggtgg atggtgttga acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct
    21001 ggaaatgctg gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg ccattcagac
    21061 cgcgctgggt ggttatcaga gcatcagtga atttattacc cctggcggtc gtctttatga
    21121 gcagcgtaac cgcgcgtggg aattgatcaa cgatattccg ggcgtttcct gcgtgaaacc
    21181 ttgtggtgcg ctgtacatgt tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga
    21241 tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc
    21301 attcaactgg ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg tcgatgatat
    21361 cgagctgtcg ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc tgtaatctta
    21421 atttcactgc cggagattgc atccggcagt gttatcccgc cacaatgacc tgatgatgtc
    21481 atcatacgta aggtcactat gaaacagagc catttttttg cccatctctc ccgcctgaaa
    21541 ctcattaacc gctggccgct catgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt
    21601 ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca aaaatcggaa atttggcggt
    21661 aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc acgatgcctc agaagtgctc
    21721 accggcgatc tccctactcc ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag
    21781 gctattgaaa aaatcgctca gcaaaaactg gtcgatatgg ttccggaaga gctgcgggat
    21841 atctttgcgc cgttaattga cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa
    21901 caggcagatg cactgtgtgc atatctgaaa tgtctggaag aactcgcggc gggaaataat
    21961 gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg aagcgcgccg cagccaggag
    22021 atggactact tcatggaagt atttgttccc agcttccatc tttcgctcga tgagattagc
    22081 caggattcac cgctgtaagc agctggagtc tgcgtcgcat caggcaataa gcgccggatg
    22141 cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat catcaccaca caaaccgcca
    22201 tcacgataat ggtgaacggt acccccagct tcacaaagtc actgaagctg taatttcccg
    22261 gacctaaaac caatgtgtta acaggtgaag aaaccggtgt cataaaggcg gcggatgctg
    22321 ccatcgccac gaccatcgcg aatggataag gcgacacccc catcgttttg gcagcagcca
    22381 gcgcaatcgg agccatcaac accgccgtcg cggtattaga gataaatagc ccaataaccg
    22441 ccgacaagac aaacaaacaa cccagcatca tatgtggccc gtaaccaccg ccaatgtcca
    22501 tcagcccttt caccgccagc gcgacacctc ccgttttctg taatgccaca gcaaacggca
    22561 tcatcccaac gatcaaaata atgctcggcc agtgaatgga tttataggcg ctttcagcat
    22621 ctatacagcg gaatttcccc atcagcaggc aggcgatgat agcggcaaca ggattaggaa
    22681 tttcatctgt cagcattaac gccaccatta gcaccagaca gaaaatggca tggggtgcct
    22741 ggctgtgcgc gggcgatgct tcactcacct cttccggtaa gttcagcgct acgaagtcgc
    22801 ggccctgttt ggccagcata ccgatcagtt tccagttacc cacaaccagg atgatatcgc
    22861 ccagcagcag aggctcatcc gccagcgaac cttccagcgc cacgccattg cgctttagcc
    22921 ccaccacatt cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca
    22981 gttctgactc aggaattaat gaaatctctg ccatgcccac atcaagggcc tggtcagaaa
    23041 aatactcgcc gcgcagtacc atcggctcca gcaattgctc actacaaaat tgccggagat
    23101 cgacatcagc cgcagacata tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc
    23161 cattaacgtt cacgataacg cgacgaaaac gccgccagcg ttcaacaccg atgacgttag
    23221 cgccataacg ctcacgtaat ttgagatcat ccagccgttg accaatcatt ggcgatccgg
    23281 ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata ttcacggata agatcgcgaa
    23341 aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt atcccctttc agcatgaaac
    23401 gcatcactaa catatacaaa atacccagca ccagcacgac caggccaata ggtgttacgc
    23461 taaagaaact gaagccgtga tagccttcac gcagcaattc actgttgact accaggttcg
    23521 gcggcgtcgc caccagcgtc atcatgccgc taatcagccc ggcaaaactt aacggcatca
    23581 tcagacgtga cggcgaagtt tgcatacgca tggcaacgct taacaccacg ggaataaaga
    23641 tagcgacaac gccggttgaa ctcataaacg cgccaagccc cgcgacggtc agcatcaaca
    23701 aaaccaacat tttgatttca ctgttgcccg caactttgac cagccatgtt cccattacgg
    23761 tggcaacacc ggtacggacc aaaccatcgc caataataaa caaggcggca atcaggacaa
    23821 cgttaggatc agaaaagcca gaaaatactt ctgggactgt cagcgttccg cttaatgcaa
    23881 acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga
    23941 cgatggcaac ggccagtaat gaaagaaccc aaatcaattc accgttcaca acttatcctt
    24001 gttaattgag ggggatgact tgattctgcc ataaaaaagc cccgacgaga cggggctaaa
    24061 tcatgatcag gtgtttcact gaataataac atcgccattt ggctgtttgg tcacagtaat
    24121 ttgctccaga ctgtggagga ccaaatcgac ctcattcagg cgcggggtat ctgccggagc
    24181 gttaaccgca atgacatggc aacccgccgc caggccagaa agcacgccag cgggagcatc
    24241 ttccaccacc acacactcct gcggcgcaag ccccagcagc tgcgcgccta acagatacgc
    24301 atcaggttct ggttttccgc gcttcactcg ctcagcggtt acaaacacct ctggtgcggg
    24361 aagccccgct attttatggc gcgctcgcgc taccggcatg gagccagaag tcacaatggc
    24421 ccacggaata cctgctttat tcaaatgatt gagtaaggcg attgcccccg gaagcgcggt
    24481 aataccttcg gtttccgtgg cctcgatttg ctccaaacgc gtaaactcgg cggcaatatc
    24541 agcctcggat ttgcccgcca taaaatggcg cagagaggtg atcgcctgtt taccgtgaat
    24601 gaaagccagc acctcttccg gcgctaaccc atgacgtctg gcccagttgc tccacgcccg
    24661 ttctaccgca ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa aacctttgca
    24721 ccgcacgcgg gcctcctcag gcgttgataa tttgattaat ttcgttggcg ctcaaatggt
    24781 actgacgcgg gcaggcatgc cacacattaa gcatgcgctg atatttttcc cacattggcg
    24841 tctgggcgtt aaaaccgtga gttccggcgt caaaatgggt atagcgccct tccacattaa
    24901 ccataaagcg gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg
    24961 tgacacgacg ttcatcgatg gattgctggt cctgcaaatt agaccaggaa acatgcaacg
    25021 catgatacat ctccataatg tcgatgatag tgcggcaggt ttcttctttc agctcgccaa
    25081 actcgcgatc cagttcacgc atctgtaatc cataaccccg ctcaatgatt gtttgcaggc
    25141 gacggtaacg ttcagcattt gccggatcga gcatagtcat catcttgtac tggttagaca
    25201 aaataagacg ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact ctactacggt
    25261 gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt aatcaggggt caattacaaa
    25321 tcatcaagga aagttttatc cagttgtttg aaggcacgct taagcgtgtc agctaatgcc
    25381 tggtaatcag gcttgccttc aacgggtgcc aacacctgtc cagactcctg caatttaccg
    25441 cgaacttcat aaaaccagtt gaggattgca gggggtaatg gcgtgacaga acgcttgccc
    25501 agccaccaca atccctgcat gggtaaactt aaggcgaaca gggcagtggc aactgccggc
    25561 ccaagctgac cgcccagggc aatctgccag cagagagtaa atacggcgat cggcggcata
    25621 aaacggatcg cataacgcgt catcttgata acgcgatttt cgacaaagac cggggcaagg
    25681 cgtttttcca gcggccacgt ctttgagtaa tgctgtcccc ggcgaaacaa gctaaaaaaa
    25741 ttaacagaac gattatccgg cgttgacatg cttcacctca acttcacata taaagattca
    25801 aaaatttgtg caaattcaca actcagcggg acaacgttca aaacattttg tcttccatac
    25861 ccactatcag gtatccttta gcagcctgaa ggcctaagta gtacatattc attgagtcgt
    25921 caaattcata tacattatgc cattggctga aaattacgca aaatggcata gactcaagat
    25981 atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa atgtcggtgt
    26041 catcatgcgc tacgctctat ggctccctga cgttttttta gccacgtatc aattataggt
    26101 acttccatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc actgaaattt
    26161 gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga atgtttccac
    26221 ctgcccgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc ggctttaggt
    26281 gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct ggcacaaaaa
    26341 ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg cggcgaaaag
    26401 tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga tgcagcttct
    26461 tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct gaaatctttc
    26521 ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca gactatgccg
    26581 gaagagtctt acctctacgc cctgccgtac aacctgtaca aagagcacgg catccgtcgt
    26641 tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa aatgctgaac
    26701 aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg ttccgtttct
    26761 gctatccgca acggtaaatg cgttgacacc tctatgggcc tgaccccgct ggaaggtctg
    26821 gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct gcacgacacc
    26881 ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg cctgctgggt
    26941 ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac gaaagaagac
    27001 gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg tgcctacact
    27061 gcgctgatgg atggtcgtct ggacgctgtt gtattcaccg gtggtatcgg tgaaaatgcc
    27121 gcgatggtcc gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga agttgatcat
    27181 gaacgcaatc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga aggtacccgt
    27241 cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc gagccgcctg
    27301 actgcctgat ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc cgccaaatcg
    27361 gcggtaacga aagaggataa accgtgtccc gtattattat gctgatccct accggaacca
    27421 gcgtcggtct gaccagcgtc agccttggcg tgatccgtgc aatggaacgc aaaggcgttc
    27481 gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg cccgatcaga
    27541 ctacgactat cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg ctgaaaatga
    27601 gctacgttga aggtctgctt tccagcaatc agaaagatgt gctgatggaa gagatcgtcg
    27661 caaactacca cgctaacacc aaagacgctg aagtcgttct ggttgaaggt ctggtcccga
    27721 cacgtaagca ccaatttgcc cagtctctga actacgaaat cgctaaaacg ctgaatgcgg
    27781 aaatcgtctt cgttatgtct cagggcactg acaccccgga acagctgaaa gagcgtatcg
    27841 aactgacccg caacagcttc ggcggtgcca aaaacaccaa catcaccggc gttatcgtta
    27901 acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg cccggatctg tccgagattt
    27961 tcgacgactc ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg caagaatcca
    28021 gcccgctgcc ggttctcggc gctgtgccgt ggagctttga cctgatcgcg actcgtgcga
    28081 tcgatatggc tcgccacctg aatgcgacca tcatcaacga aggcgacatc aatactcgcc
    28141 gcgttaaatc cgtcactttc tgcgcacgca gcattccgca catgctggag cacttccgtg
    28201 ccggttctct gctggtgact tccgcagacc gtcctgacgt gctggttgcc gcttgcctgg
    28261 ctgccatgaa cggcgtagaa atcggtgccc tgctgctgac tggcggctac gaaatggacg
    28321 cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg cctgccggta tttatggtga
    28381 acaccaacac ctggcagact tctctgagcc tgcagagctt caacctggaa gttccagttg
    28441 acgatcacga gcgtatcgag aaagttcagg aatacgttgc taactacatc aacgctgact
    28501 ggatcgattc tctgactgcc acttctgagc gcagccgtcg tctgtctccg ccagcgttcc
    28561 gttatcagct gactgaactt gcgcgcaaag cgggcaaacg tatcgtactg ccggaaggtg
    28621 acgaaccgcg taccgttaaa gcagccgcta tctgtgctga acgtggtatc gcaacttgcg
    28681 tactgctggg taatccggca gagatcaacc gtgttgcagc gtctcagggt gtagaactgg
    28741 gtgcaggcat tgaaatcgtt gatccagaag tggttcgcga aagctatgtt ggtcgtctgg
    28801 tcgagctgcg taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag ctggaagaca
    28861 acgtggtgct cggtacgctg atgctggaac aggatgaagt tgatggtctg gtttccggtg
    28921 ctgttcacac taccgcaaac accatccgtc cgccgctgca gctgatcaaa actgcaccgg
    28981 gcagctccct ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt tacgtttacg
    29041 gtgactgtgc gatcaacccg gatccgaccg cagaacagct ggcagaaatc gcgattcagt
    29101 ccgctgattc cgctgcagcc ttcggtatcg aaccgcgcgt tgctatgctc tcctactcta
    29161 ccggtacttc tggtgcaggt agcgacgtag aaaaagttcg cgaagcaact cgtctggcgc
    29221 aggaaaaacg tcctgacctg atgatcgacg gtccgctgca gtacgacgct gcggtaatgg
    29281 ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct accgtgttca
    29341 tcttcccgga tctgaacacc ggtaacacca cctacaaagc ggtacagcgt tctgccgacc
    29401 tgatctccat cgggccgatg ctgcagggta tgcgcaagcc ggttaacgac ctgtcccgtg
    29461 gcgcactggt tgacgatatc gtctacacca tcgcgctgac tgcgattcag tctgcacagc
    29521 agcagtaacc tcgtcatcat ccgcagcttt gcgctgcgga tatctgaacc ggaaatagtc
    29581 actatttccg gttttttatt ctcttaattt gcattaatcc tttctgatta tcttgcttaa
    29641 ctgcgctgca tcaatgaatt gcgccatctc actttgcata cttaccactt tgttttgtgc
    29701 aagggaatat ttgcgctatg tccgcaatca ctgaatccaa accaacaaga agatgggcaa
    29761 tgcccgatac gttggtgatt atcttttttg ttgctatttt aaccagcctt gccacctggg
    29821 tagttccggt ggggatgttt gacagtcagg aagtgcagta tcaggttgat ggtcaaacaa
    29881 aaacacgcaa agtcgtagat ccacactcat ttcgccttct gactaacgaa gcaggcgaac
    29941 ctgagtatca ccgcgtacag ctgttcacga cgggcgatga acgcccgggc ctgatgaact
    30001 tcccgtttga agggttaacc tcaggatcga aatacgggac agccgttggc atcatcatgt
    30061 ttatgctggt gattggcggc gcgtttggca ttgtgatgcg tacaggaacc attgataacg
    30121 gtatcctggc gcttattcgc catacccgtg ggaatgaaat tctctttatt cctgcgctgt
    30181 ttattctgtt ttcacttggc ggcgcggtat ttggtatggg ggaagaggcc gtcgcctttg
    30241 ccattatcat cgcaccgcta atggtccggc tgggctatga cagtattacc accgtcctgg
    30301 tgacctatat tgccacgcaa atcggttttg ccagttcgtg gatgaacccg ttttgtgtgg
    30361 tcgttgctca ggggattgcc ggcgttccgg tgctttctgg ctccgggttg cgcatcgtgg
    30421 tgtgggttat cgccactctg attggcctga tctttaccat ggtgtacgcc tcacgagtga
    30481 aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt gaaaagcagg
    30541 cggatgttga acaacgtccg tttacctttg gtgactggct ggtattgatt gtcctgaccg
    30601 ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt ccagaaattg
    30661 ccagccagtt cttcaccatg ggtctggtga ttggcatcat cggcgttgtt ttccgcctta
    30721 acggtatgac ggttaatacc atggcttcat ccttcaccga aggggcgcga atgatgatcg
    30781 cccctgccct gctggtgggt ttcgccaaag ggattttgct gctggtcggt aatggtgaag
    30841 cgggtgatgc cagcgtgtta aatactatcc tcaacagcat tgccaatgcc attagcggtc
    30901 tggacaacgc ggtcgcggcc tggtttatgt tgctcttcca ggcggtattt aatttcttcg
    30961 tgacgtccgg ttctggtcag gcggcgttaa ccatgccgtt actggcaccg cttggcgatc
    31021 tggtcggtgt taaccgtcag gttaccgtgc tggctttcca gtttggtgat ggcttcagtc
    31081 acatcattta cccgacctca gcttcgttaa tggcgacgct cggtgtttgc cgggtggact
    31141 tccgtaactg gctgaaggtg ggcgcgaccc tgcttggact gctgtttatt atgtccagcg
    31201 tcgtggtgat cggcgctcag ttgatgggct accactaaaa atgttaagag ccgcaattgc
    31261 ggctcttttt tcattctgcc gtttcagtct ctacggcttc atttttggca ttgcgcttca
    31321 tccacaacgc tagcgctttc agcgagtctg gagtgaactc atcgcagcgt gcggtgattt
    31381 cttccggcgt cagccagcaa acttcactga cttcatcttc ctgtagggcg aagggaccgt
    31441 gagagacgca gctgaacaat gcgccccaga cacggcaatt tttatcttcg aaatagaact
    31501 gcccgtgctc ggcaaagggg acaccggcaa tgcccaactc ttcttccgct tcgcgacgcg
    31561 cggattccag cagttgctca tcggcctgga ctacaccgcc agcggtcgca tctaacatgc
    31621 cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag aattttgccc atgccatcat
    31681 gcacgacgat gtaagttgca cgatgacgca gacactgtgc ccgcatttgc tcccggctgg
    31741 cttgtgcaat gacttcgttc tcttcattga caatatccac ccattcagta cttgccaaac
    31801 gacgctgttc cattatcagg aaaccttctt tttctggcgc tcttacggcg cattttgagt
    31861 tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat actttgatca ttgagtgcga
    31921 ttacactaag tacgtcatta tccagcatgc cataactcgc cggattaccg cctttcggaa
    31981 tactcaccga gccggggttg aagtggaaaa tctccccccg ctgttctgct actggcagat
    32041 gggtatgacc gtaaaccagc acatcgttct ggtttaaagc aggcagattt tccgggccaa
    32101 aaagatgacc atgcgtcaaa aacagacgtt gtttttccag taatacctgt tgccacggcg
    32161 cggttatcgg gaaatgcagc agcatttgat ccacttcgct gtcgcagttg ccgcgcacag
    32221 cgataacctt atgtgccact tcattaagcc gttcagcgac tttggctggc gcgtaaccct
    32281 ccggtaaagc attacgcggg ccgtgattca acacgtcgcc aaggatcacc agccactggg
    32341 caccgctttg ggcaaacaac tccagaacac gttccgtcgc cggtaacgac ccatgaatgt
    32401 ccgatgcaaa catcagtttc atcactcact cctcgtcgaa aaaacgtaag ctatgatact
    32461 ggattctgtc gttgctatca gccagattgc ttcgctgaaa gtgcaataaa acgctggaca
    32521 gacgctcgct gccactggaa tgttgcataa tccaccaggc attctggcac ctcatcgcca
    32581 tgtagtacca ggcggttaat cattagcgcc agatcagtat cagcaatgca ccattcacca
    32641 aataaattcg gctgacccag tactaacaaa tgttctgcca tcgcgaacag tttctctgca
    32701 ctggcttttc cctcggccgt tagtggcgct tttttcgccc ccgcaaagac aacatctgtc
    32761 ggacgctctt cgcggatggg catcagatcg ctgcgcaacc aggcctgaat ctgtcgtgca
    32821 cgcgcacgat tttctaaatc aagcggataa atacgttccc aggtcggtgg cgcaaatcga
    32881 tcttccagat actccgcaat ggcagaagat tcactcaact caaaatcatc gatttgtaat
    32941 aacggcacac ggcgtgtctg accgtaacct tgccacgtcg gttgcaaatg ttcaccgctg
    33001 tcgaggtcga tggtcttgat atgaaacaac aggccttttt cctgcaacgc cacccaagcg
    33061 gataacacat aaggggagaa aaaatgggca tctgaccaaa gcgtgatagc gggtttactc
    33121 attatgtcct cggcaataaa gggggctggc tatcaaacta tagagccttt ttgtcactgt
    33181 cacgtgacga atactcacgt aagtcgccgc gctttctata attgtggtgt tcagatgatt
    33241 agacaaccgg agctccaatg atcgatctct atttcgcccc gacacccaat ggccacaaaa
    33301 ttacgctgtt tctcgaagaa gcagggctgg attatcgctt gataaaggta gacctgggga
    33361 aaggcggtca gtttcgcccg gaatttttgc tcatttcgcc taacaacaaa attccggcaa
    33421 ttgttgatca ttctccagcc gatggcggcg aaccgctaag cctctttgaa tctggtgcca
    33481 ttttgttgta tctggctgag aaaacggggc tctttttgag tcatgaaacg cgtgaacgcg
    33541 ccgccacatt acagtggtta ttctggcagg taggcggact ggggccgatg cttgggcaaa
    33601 atcatcattt taatcacgca gccccccaaa ccattcctta cgctattgaa cgttatcagg
    33661 ttgaaactca gcgtctgtac catgtactga acaagcggct ggaaaactcg ccctggctgg
    33721 gaggcgagaa ctacagcatt gcggatattg cctgctggcc gtgggttaat gcctggactc
    33781 gccagcgaat agacctcgct atgtatccgg cagtcaagaa ctggcatgag cggatccgtt
    33841 cgcgccctgc caccgggcag gcactgctaa aagcacaact cggtgatgag cgttcggata
    33901 gttaacagaa acaggttctc gtgtattatt tcatcctaag taaaacaacg gagaacctgc
    33961 aatggcacaa cctgccgcta ttattcgtat aaagaacctt cgtttacgta catttatcgg
    34021 aattaaggaa gaagaaatta acaaccgtca ggatattgtt atcaatgtga cgatccacta
    34081 ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt atcgcaccgt
    34141 aacgaaaaac attattcagc atgtagagaa taaccgtttc tctttgctgg aaaaattaac
    34201 tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg aagtggagat
    34261 cgataaactg cacgcgctgc gctacgccga ttcggtatcc atgaccttaa gctggcagcg
    34321 ttaaccgcca tatcgggagg ctgcatgaac atagtgatca ccggagggac gggattaatt
    34381 ggtcgccatt tgattccacg tttgctggag ctgggccatc aaatcacggt aatgacgcgt
    34441 aacccgcaga aagccagttc cgttctcggc cctcgggtga cactatggca agggcttgcc
    34501 gatcaaagca acctcaacgg cattgatgcg gtaatcaacc tggccggaga accgattgct
    34561 gataaacgtt ggactcacga gcaaaaagag cgtctctgcc aaagccgctg gaatatcacg
    34621 caaaaactgg tcgatttgat taatgccagc gacacgccac cgtcggtact catttccggc
    34681 tcggcaacgg gctattatgg cgacttaggt gaagtggtgg ttaccgaaga ggaaccgccg
    34741 cataacaaat ttacccataa actctgcgcc cgctgggaag aaattgcctg tcgggcgcaa
    34801 agtgacaaaa cgcgagtgtg cctgctgcgt accggcgtag tgctggcacc ggatggcggt
    34861 attctcggta aaatgctgcc gccgtttcgt cttggcctgg gcgggccgat tggttccggt
    34921 cggcagtatc tggcctggat tcatatcgat gatatggtca acggcattct ctggctgctg
    34981 gataacgagc tgcgcgggcc atttaatatg gtttcgccct acccgatacg caatgaacaa
    35041 tttgcccatg cgctcggtca tgcgctgcat cgcccggcta ttttgcgcgt ccccgcaacc
    35101 gccattcggc tgttaatggg cgaatcttca gtactggtat taggtggaca acgcgcgctg
    35161 cctaaacggc tggaagaagc gggttttgcg tttcgctggt acgatttaga agaggcgctg
    35221 gcggatgtcg ttcgctgatg tggtttactg caaacatccg ccagttaact cccggtgtta
    35281 cagggttagt ggctttgcgc gataagatcg tctggtgaaa gtcgggtcag cgtcaggatg
    35341 acttcgtggt caaatccttt ttccagcatc tcctgagcaa tacgcagagc ttcttcgtgt
    35401 ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa tggtcatcag tttctccttt
    35461 tcttgtggtg cgcgttctgt tatctcacca ataaatgcac ggaaacgctg ggcatctccc
    35521 gtttgtaata cataattaaa cagggctttt agctgtctgt cattagtgtt ccctgtaact
    35581 agcagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat atgtttctga
    35641 attaactcca acagcgccat tttacgatgt tgcataatct cgtcatccgg caccacggta
    35701 atatccacca acggaaaagc cgatgaatat attttacggg caatcgcagg ctcggcaaat
    35761 tcatcaagcc agcagagtga ataaggataa ggacttctgc aaccatgata aaacagcatc
    35821 gggatcacca atggaagctc tttatagccc acatcaagat ggttttgcat tgccgcaatg
    35881 gaataacgca tcatgcgaaa agccattaat tcttccggct tactttggtg ctctatcact
    35941 acataaagat aacccactcc ctcctgcgtt ttcacagacc acaagaggtc ggaataatat
    36001 tgccgcaggt cttcatcaat aaaactgttt ggttccagtt taagcgtcgt taaatcacac
    36061 agtttgcgca gcggcgcggg aagatgaata tcaataaaat cccgcgcggt gtctggatgg
    36121 cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat tgtcatggcg
    36181 ttccgtcacc cctcaaattt aagatgacgc gacaataacc acgattaaca cccaatgcag
    36241 caggcagttt ttatctttgg acagcgaatt cagaagtatt tttagcggac tgcaactttt
    36301 acatttcagc ggaagctgac tcgcaaattc cccttgaaaa tactccggca gcgttagcca
    36361 ccggagaggc aattatttca gcgaaccttt aaggaactgt tgcagacgag ggctttgcgg
    36421 gttgccaaat aactgctccg gcgcgccttc ttcctctatt ttcccctgat ggaggaaaat
    36481 gacgtgagta gatacatggc gagcaaagcc catttcgtgg gtcaccacca ccatcgtttt
    36541 tccctcttcc gccagctgct gcataatgcg cagcacttca cccaccagct ctgggtcaag
    36601 ggccgatgtc ggttcatcaa acaacagaac ttccggttcc attgccagcg cccgcgctat
    36661 ggaaacacgc tgttgctgac caccggacag gtgcaccggg tatttcccct gcgcacgttc
    36721 gtctatcccg acttttgcca gatacttcac cgcccgctcg cgcgcttcct gcttgctcag
    36781 gcccaacacc tgaatcggcg cttccatgac gttttccagc accgtcatat ggctccagag
    36841 attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt aagcgcagtt gatttttatc
    36901 ggcgactttg agttgaccgt ctttgtcgcg caccagattg atcgtctggc cattgaccac
    36961 gatcgaccct tcactcggtt tttcgaggaa gttaatgcag cgcagaaagg tacttttccc
    37021 cgatcccgac gatccgatga tgcttattac atctccggca ttcgcttgca gtgacacccc
    37081 tttcagcact tcgtgttcgc catagcgttt gtgcaaatcg ataacgttta atttattctc
    37141 ggacatcgta ttctcagtgc gttgaagaag gtttcacatg ctgcaaccag cgtttttccg
    37201 ctctgcgaaa gaggctgatc aggacataag agatgattaa atagagcacc gcggcaatgc
    37261 cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc gcgggctatt ttcagcagat
    37321 ccggcaccgt ggcagtaaat gccaacgcag tagagtgcag catcaggatc acttcgttgc
    37381 tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat aatgcagcga tacattttaa
    37441 aagtcgagaa gccataggct ctggcggctt caatttcccc atgcggcacc gaacggattg
    37501 ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag cgtcagcgcc agcacggtac
    37561 agttcaggcc actgcggaaa aaggcgttaa gaaactcggt ccccttaaca atctccagcg
    37621 tatacatgcc ggaatagaac accagcaact gaacatacag cggcgtaccg cgaaaaatat
    37681 aggtaaataa ccagattgga aactggatgt atttattact ggagacacga ccaatcgcca
    37741 gaaacagcgc caggactccg cctatcacta ccgacaaaat aagcagccac agagtgatcg
    37801 ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg tttccagtat tcatgtaaga
    37861 tttcgatcac aggtcagccc tcttcacacc cacagagtag cggcgctcaa ggaacagcag
    37921 cacaccattg gaaacggtgg tgaaaaccag gtaaatcacg ccacagacga tagcgaaata
    37981 gaacggttcc caggtacttt tgcctgccag ctgcgtggct ttgaccacat cttccaggcc
    38041 gagtaacgaa accagtgcgg tagatttgag gatcacctgc cagttgttgc caatgcctgg
    38101 cagggcgtaa cgcatcatcg ccggaaacat gatccgccga aacacttgcc cacgagtaaa
    38161 accgaacgcc ctcgccgcct ctatatgtcc tttcggcact gccataaaag caccacggaa
    38221 cgtttcggta aaataagcac cgtagataaa accgagagtg ataataccag cgaccatcgg
    38281 atcgatatca atctgcccga cgcccatcgc ctccgtcacc gtgtttagcg caatctgcaa
    38341 accgtagaaa atcagcagca tcaacactaa gtccggcacg ccacgaatca gcgtggtgta
    38401 cccttcgaaa atcagccccg aaagccgatt ttgcgagagc ttaccgccag cgccaattaa
    38461 accgatgatt acagcgagca ctacagagct gatagccagc tccagcgtga cgagcgcacc
    38521 ctgtaaaata acacctgaaa acccatacaa catgctgcct gtcctgtcgt gtgtggtgaa
    38581 ttaccgcctc ttgtctccct ccggttaccc gaagggagcg ggctttcttt caccgatggc
    38641 gattaaccac cataaacatc aaaatcgaag tactttttcg ctaatttctc gtaagtaccg
    38701 tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc gcagttcgtt atcttcttta
    38761 cgcaggccca tgccggtccc tacgccaaac agtttttcat ctttaacaga cgggccaccg
    38821 aatttgtaat ctttaccgac aggttgtttg aggaaacctt cgctggcagc gacctcatcc
    38881 tggaacgcgg catcaatacg tccggcagtc aggtcagaat aaatgttgtc ctgcccctga
    38941 tacgagacga tttcaatgcc ttttggtgcc caatgttcat taccgaacgt ctcctgggta
    39001 gtgccctgca atacgcctac ccgtttgcct ttcagcgact cgactgtcgg ctgaatgtca
    39061 gaatttttcg ccaccaccag acgagaatcg gcagcgtaca gtttgtcggt gaaggctatt
    39121 tcttgctgac gtttttccgt aatggaaagc gatgacatga tggcgtcaat cttcttcgct
    39181 tttaaggacg ggattaacgc atccagcgga ttttcgacaa acgtacattg cgtattgatg
    39241 cgtttgcata attcctttgc cagatcgata tcgaagccaa ccagttcgcc ttgtgaattt
    39301 tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc ggatgttttg cggaatcgca
    39361 gcaaatgccg cagttgcgct ggagaaggcc agaaccagag agagcgatag caccagtttt
    39421 ttcataatgt cctcaactga cagtcttttt atagggaata tttacaggtt tgttacactt
    39481 atcgtgccat aaaattgacc atcaaggcaa aatatcccgc cccgttagac gtattatttt
    39541 tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga tagtgcaaca aacctggcgt
    39601 gcaccatgat gatcattcca tcaggtacag cttccctgcg acgtatcagt caccgtagac
    39661 attaaagtca aaatactttt tcgccatctt gtcgtaggtg ccgtcctgac gcagctcgcc
    39721 aagcgcctta ttgaaggcag ccgtcagttc agcatcatct ttacgtagcc ctacaccggt
    39781 gccatcaccg aagtattttt tgtcttttac tgatgagcca gcaaaggcga aatctttacc
    39841 agcaggttgc ttgaggaatc cttcgctggc agcaacttca tcctgcagtg cagcatccag
    39901 acgtcctgca gccagatcgg aatagaccaa atcctggttg gcataggcca ccacatccac
    39961 acctttgcta cgccaggtct cgttagcgta agcttcctgg gttgatccct gcagcacacc
    40021 aacatgttta cctttcagtg agtccagcgt tggctgaatc ggcgaacctt tggcggcaat
    40081 cagacgggaa tctgcggcgt acagcttgtc ggagaaggca atctcctgct gacgtttatc
    40141 ggtaatggaa agcgacgaaa taatggcgtc gatttttttc gctttcagtg aggggatcag
    40201 cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc atccgtttgc acatctcgtt
    40261 accgagatcg atatcaaagc cgacaaagtc acctttggca tctttcgacg agaacggtgc
    40321 ataggtggta tcggttccga tacgtaccgt ctccggcaat gccgcatagc tggaagccgc
    40381 tgcggaaata ccgactaaca aagacagagc gagaatcgac ttcttcatac ataaccctca
    40441 agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg tttgcaggct tgttcatgca
    40501 ggtcttatgc catcttgacg acaacagtaa cattcaacgt taaatatgtt aataagacgt
    40561 tgcattattg tcctaaagtt gaagatagca ggtatagcgg ttgaatcgca gcgtttcgat
    40621 tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt gcaaaattgc cctgaaacag
    40681 ggcaacagcg gagttatgcg ccctgccagc gggcaaagag atcttcagga agggttatcg
    40741 caaactggtc aagaacacga ttaaccgtct gatttatcac atcatcaagg gattgcgggc
    40801 gatgataaaa cgccggaacg ggaggcataa tcaccgcacc gatttctgct gcctgcgtca
    40861 ttaaacgcag atggcctaag tgcaatggtg tttcacgcac gcagagcacc agtgggcgac
    40921 gctctttcag caccacatct gccgcacggg tcagtaagcc atcagtatag ctatggacaa
    40981 tgccggacag ggttttgatt gaacagggta aaatcaccat ccccagtgtc tggaaagaac
    41041 cggaagagat gctggcggca atatcgcgcg catcatgcgt gacatcagct aatgcctgca
    41101 cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg gcgcgccgcc tggctcatta
    41161 ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg taataagcgc acaccataaa
    41221 tcgcgccgct ggcaccgctg atgcccacaa taagtcgttt cataaatagt tgccctgtca
    41281 gacttgcagg cagactttgc aggatttcgg cgggagttgc aagtcagggt gccagaccgg
    41341 caccctgagc gaaggcatca tccttcgtta tgcatttcga gattttccac ttcgttctga
    41401 cgttgcactg ctttggcgtc gtcattacgt aaagtatcga ggaaatcgag gtagccctga
    41461 tcaacatctt tggtgacgta gacgccgttg aacaccgagc attcaaactg ctggatatcc
    41521 gggttttcag cgcgaacggc ttcgatcaga tcgttcagat cctggaaaat caacccgtca
    41581 gcaccgatga tctggcgaat ttcatctact tcgcgaccgt gagcgatcag ttccgtggcg
    41641 ctcggcatat caataccata aacgttcggg aagcgaattt ccggtgccgc agaagcgagg
    41701 tacactttct tcgctccggc ttcgcgtgcc atctcgataa tctgctcaga agtggtgcca
    41761 cggacgatag agtcgtcgac cagcaggacg tttttatcgc ggaactcggc gcggttggcg
    41821 ttcagtttac ggcgcacgga cttacgacgc agctgctgac ccggcatgat gaaggtgcgg
    41881 ccaacatagc ggtttttaac gaagccctgg cggtacggtt tgcccagaat acgagcaatt
    41941 tccagcgcga tatcacacga ggtttccggg atcgggatca ccacgtcgat atccagatct
    42001 tcccattcgc gggcaatttt ctcgcccagc ttcgtgccca tattcacacg cgcgctgtaa
    42061 acggaaattt tgtcgatgaa cgagtccggg cgagcaaagt atacatactc aaacaggcac
    42121 ggattgctga ccggattgtc agcacattga cgggtaaaca actgcccttc ttcagtgatg
    42181 taaatcgctt cgccaggcgc gacgtcacgc aggaaatcaa agcccagtgt atcgagcgca
    42241 acgctttcgg aagcgaccat atattctgtg cggttctcgt caatatcacg ttttcccaga
    42301 accagcggac gaataccgtt tggatcgcgg aaagcaacca taccgtggcc gataatcatc
    42361 gccacacagg catacgcgcc gcggattaag cggtttgtag cagcgatggc ggcgaaaatg
    42421 ttgtcggctt caagcgggta gtggcggaag ttgtccagct cgctggcgaa gatattaagc
    42481 agaatttccg agtcggaagt ggtgttgatg tggcggcgtt tttcttcaaa cagtttttta
    42541 cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa gcgtaatgcc atacggagag
    42601 ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc cagccgtagg gtaacgcaca
    42661 tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc gagcttcaaa tacatcgctc
    42721 accagcccgt tcgctttacg caaacggaag cagttattgg catctatggt gatgatgccg
    42781 gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat cataaatcga ctggttaacc
    42841 ggcataacac cggcgatacc gacaataccg cacatacgtc tttttcctcg ttaagccaca
    42901 tctcagagca cttacgctct gggcaagaaa cttgacgagc tttgcagata atcaaaaaac
    42961 catctgatga taaaactgaa ctgcggaatc agttgtgatt tactccagtc ttcgcttttc
    43021 gacaccccgg taaaggagtc gagaaagaag agaatagcag caacaatcaa cacaccgcgc
    43081 aatgcaccga aacagacacc cagcacccga tcggtgcctg acaaccccgt tttctccacc
    43141 agcaggccta tcacgaagtt cacgatagcg ccaacgatca gggtggcgat aaacaggacc
    43201 gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc ccgtaaacca gactgacagg
    43261 taagtgtagt aatgactggc aacaaagaaa gcacaacccc atgtcaccag cgataacgct
    43321 tcacgaacaa agccgcggat caggctaacc agagaggaaa aagcaatcac cgcgattatg
    43381 gcgtaatcaa tccagaccat atgtgtccca cgattttacg ccctgtcatc ctgttcgggg
    43441 cgcattctaa cagaaaaaga aaacgtttgc gtagggattt ccttcccgcg catcaataaa
    43501 aatggcgctg aaaaaatatt caacgccatc gactttttat gcctttgcgg catcggacaa
    43561 tgcgtgtcgg atgcggcgta aacgtcttat ccgacctacg gttctacccc tgcgtaggcc
    43621 tgataagacg caccagcgtc gcatcaggca acgcctgtcg gatgcggcgt gaatgcctta
    43681 tccgacctac ggttctaccc ctgcgtaggc ctgataagac gcgccagcgt cgcatcaggc
    43741 aacggctgtc ggatgcggcg taaacgcctt atccgaccta cggttctacc cctgcgtagg
    43801 cctgataaga cgcgccagcg tcgcatcagg caagaccgta ttaattcggc gtatagccca
    43861 ttaccacgcc acttaagcca gaaagttgct tcaactcacc cagcgaacct ttcagcttat
    43921 ctttcgaggc atccggccca accagaatac gggtaatttt accctgcact ggcgtggatg
    43981 gcgacgtata aacccgataa ccggcaccgc gcagcttacc gacaatctca ttcactttat
    44041 cggcattttt cagcgcgccc agttgcacaa cataggcttt acccgttggc gctgcttttt
    44101 cttccacgac cggcttcggt tctggcttcg gcgcaggtgg tgcttcaacc ttcggcttag
    44161 gcggctccac cggtttcggc ttcggtggga cgaccggtgc aggttccggt tcaaactcgg
    44221 tgttattggc tgcaatagtg gccggatcga gcgacggtgc tgcggcatca cctgcccgca
    44281 cctcttccgc tgcgccttcc ggcggttgcg tcggcaacgc ctgggtggcg gctggcatca
    44341 tatcaggctc atcgcgatcg cccgcttttg gcaccagcgg gatagccgca aactcatcct
    44401 gataatgttt tttctgcccg tccagcagcc ctggaagtac aatcaccccc agcgccacca
    44461 gcacgatcgt gcccactaac cgattctgaa acttacttgc caccgcttct cctcgcgtca
    44521 atcacttcca tgacatgtgc gaccgtgtgg aacgaaccac acaccagcac ggtgtcttcc
    44581 gctttagcgt ccgccattgc ggcatcccat gcctgcgcaa cgctatcaaa tgatttgccg
    44641 ttacccaaat gctcaagcag ttgttctgcc gtggcaccgc gcggcccttc cagtggcgca
    44701 caataccagt catcaaccac gcttttcaac caggccagag ttccggcaat atctttatca
    44761 tgtagcatac cgataaccgc cagcacgcgc ccgtttttcg gtagcgtttt catacgcccg
    44821 gtgagatatt ccgccgcatg tggattatgc gcgacatcaa aaataacgcg tggcgactcg
    44881 ctcacaatct ggaaacgtcc cggcaaaatt gcgctggcaa tcccgtcgcg aatggcattt
    44941 tcactgacct ccagcccgct ggcacgcagt gccgccagcg ccgttgcggc attcggttgc
    45001 gggacaagcg gcaacggcag gttttccagc gtgccgtgag catcgctaaa cgcccagtca
    45061 tgatcggtga cggaatagtt ccactcaacg ccccgacgtt gtaacagtgc acctttttcc
    45121 tgcgccacat cagcaatggt agaaggcatt tccggctcac cgacaattgc cggtttttcg
    45181 ctgcgaaaga tgcctgcttt ctcgcgacca atactttcgc gatctggccc cagccagtcg
    45241 gtatgatcca gcgcaatact ggttactacc gcgacatcgg cgtcgacaat attggttgcg
    45301 tccagacgac cgcccagccc tacttccaga atcaccacgt caagttgtgc ctgtttgaac
    45361 agccacaacg ccgacagcgt accgtactcg aaataggtca gggaaatatc accgcgtgcc
    45421 gattcaatct ccgcaaaaga ggcggtgtgg gccgattccg gcaattcctg accctgcaca
    45481 cgtacgcgct cggtataaca caccagatga ggcgaactgt agacgcccac tttgtaccct
    45541 gccgccatca gaatcgactc cagcgtacgg caggtggtgc ctttgccatt cgtacccgca
    45601 acggtaaaca caaatggcgc tggtttcaga acgccaagac gcgccgcgac ctggctcacg
    45661 cgctcaaggc cgagatcgat agttttactg tgcaggtttt ccagataaga aagccacgaa
    45721 gccagaggcg acgcggcttg aggagtgcgt ttgataatca tggtatccgc tgattcgtta
    45781 cggtgagaat agcaaaaggg cagagccagt ggccctgccc ttatcagtta tcaggcctca
    45841 ggttcctgat ccggtaccgg gggtaccact acgccttcac gcggcgcttc aggattcggc
    45901 gctggcagat tcatcaactt cgccagaatg ctcgccagtt tcaggcgcat ttccggacga
    45961 cggacgatca tgtcgatcgc gcctttctcg atcaggaatt cactgcgctg gaatcctggc
    46021 ggcagttttt cgcgaacggt ctgttcgata acacgcggac cggcaaagcc gattaacgct
    46081 ttcggttcag cgatgttgag atcgcccagc atggcgaaac ttgcagaaac accgcccatc
    46141 gtcgggtcgg tcagcacgga gatgtacggc aagccgcgct cctgcatttt tgccagtgcc
    46201 gcagaggttt tcgccatctg catcagcgac atcagtgctt cctgcatacg tgcgccacca
    46261 gaggcggaga agcagatcag cgggcagtta tcttccagcg cctgctcaac ggcacgcacg
    46321 aaacgtgcac ccacaacaga ccccattgaa ccgcccataa aggcgaactc gaatgccgca
    46381 gcgacaaccg gcattccata cagagtgcct ttcatcacca ccagcgcatc tttttcgccg
    46441 gtttctttct gcgcagatgc cagacggtct ttatacttct tggagtcacg aaacttcagc
    46501 acatctttcg gctcaagctc gctacccagc tccacaaggc ttccttcatc taacaggcta
    46561 tgcaggcgat tacgcgctgt catacgcatg tgatggtcac acttcggaca gacctcaaga
    46621 ttacgttcca gctcagcgcg gtataaaacc tgaccgcagc tatcacactt agtccacacc
    46681 ccttcaggaa tgctcgcctt gcgggtggga gtaatgttgc ttttaattcg ttcaatccag
    46741 ctcattaggg acctttctgt ctgaacctgg ttcgatgcca gttttatctt tggggacgca
    46801 taatgccatt tttgccccca acagaccatg aatgttgcac attaaaacat aacagcccga
    46861 aactttggat aaaaaagtgg tcgaaccgcg gagttacttt ttattttgcg gcgcgtgccg
    46921 cagcgcgttt gtgacggatt atttcgatga cgcccggcaa aatagaaacc acaataatcc
    46981 cgacgatcag cagcttaagg ttatcctgaa ccatcgggat tgtaccgaag aaatagcctg
    47041 cgtaggtaaa aagcagtacc cacaacagtg cgccgatcac gttataagca gcgaaatgac
    47101 ggtacgacat gtggcccatt cccgcaacaa acggggcgaa cgttctgacg atcggcacaa
    47161 aacgggcgag aataatcgtt ttgccgccat gtttctcata aaactgatgg gttttatcga
    47221 gataactgcg acggaaaatt ttcgaattgg gattactgaa taacttttca ccgaacaagc
    47281 gcccgatagt gtaattgacc gcgtcaccca caatcgcggc gatcagcatc agcaccacca
    47341 tcatatggac gttgagatcg ttggtttcca gtgatgccaa cgctccggca acaaacagca
    47401 atgaatcacc cggtaaaaac ggcgttacca ccagaccggt ttcacagaac aaaattaaga
    47461 acaaaatggc ataaacccag acgccgtact ccgcgaccag ctctgccaga tgcacgtcaa
    47521 tgtgcaggat aaaatcgatg aggaaataaa tcaggtccat atttgcctat gccttgtact
    47581 cgtcatcttt caggctgtaa ctacgttggc tacagcctga aatgttccga gcattattct
    47641 cgttagtccg ccagaaatag cgggcccatt ggcggttttg gaagatcata ccggtcaggg
    47701 taatccaccg cgaccagata cagcccttcc gcttttgccg ttgctgccgc cagcgttctg
    47761 tcctttgccg ccagcaactc tgctatccag ctctccggct ggttgtgggc accgacttcc
    47821 atcaggctgc cgacaatatt cctgaccata tgatgtacaa aggcattcgc tttgatatca
    47881 accaccacat aaggaccgtg acgcgtgaca ttaatatgca taacgttgcg ccacggggtt
    47941 cgggactggc actgcaccgc acggaacgag gtgaaatcat tctcgcccag caagcactgc
    48001 gcagcccgat gcatccgttc agcgtccagc ggttcgtaaa aatgggttac ccctttactc
    48061 agtaccgccg ggcgcagccg atgattgtag atgatgtagc gataacggcg agccgtggcg
    48121 ctaaatcggg catgaaaatc atcaggtaca gctttaaccc aacgcacggc gatgtcacca
    48181 ggtaaattcg catttacgcc caacgtccac gcagcgtctt tgcgctgcgc ggtagtttcg
    48241 aaatgcacaa cctgcccggt accgtgtacc cctgcgtcag tacgcccggc gcagaagacg
    48301 gtgatgggtt cgttcgccac ctgggagagc gccttttcca gcttctcctg cacactgcgg
    48361 acttcattct gccgttgcca gccgtaatac ttactgccgt cgtactcaat gcccagcgca
    48421 attttataaa ctggcggttg ttgctggtcg gacattagta cagatactcc tgcaccagtt
    48481 tctcggcgat ttttactgcc atcagcgcgc cgccaaagcg aacgttatcg gccaccgacc
    48541 agaactggac ttgctccggc ataccgtagt cattacgcac gcagccaaca gaaagatgcg
    48601 gcgtacccga agcatcacct acctgagttg ggaattcgtt ctcttcagag agcacaatat
    48661 cttccccttg aacaaacgca tcacgcgctt cttctgctgc cagtggacgc agagcttcaa
    48721 agttgaccat ctgggcatga ccgtagaata ccggtgcctg gacgacgctt gccgaaatca
    48781 tcagtccttc gtcctgcagg attttgcgta cttcgtcaac gatacgacgt tcttcacgca
    48841 cgctaccttc gctatccggc agtaacggca gcatgttgaa cgcgagctga cgcccgaaga
    48901 aatcttcttc gtcaatcgga atgccgttga gcaatttcgc actctgcccc gctaacgcat
    48961 cgaccgcttt tttgccctgg gcggaggctg aaatcaggct ggtaacactg atacgcgaca
    49021 aaccgccctg atcgattaac ggtttcagtg ccgccagcag ctggctggtc agactgtctg
    49081 gtacggcgat gacattccgg ttgcggtaat cggtcagtac aaacgggttt acttccggca
    49141 ccaccagcgg tacgtcgggt tcaagagcaa acaatccgct gctgtcgatc accaggcaac
    49201 ctgagttggt cgcttcttca acccaggctg cggtagcttc tttgcctgcg acaaaaaatg
    49261 ccagctgcgc ctgcgtccag tcgaattcag cagcatcctg cacggtgatt gtcttaccac
    49321 caaagcgcaa ttgttcgcct gcgctttcgt tacgtgccag tgcataaatt tccccaaccg
    49381 ggaactgacg ttcagccagc gtttcaagca gggcttcgcc cacagcgcca gttgtgccca
    49441 ggacggcaat gttccagcct tcagacatgg tggtttactc cagaaatagc aaagctccct
    49501 gccaatgtta tagcagagag catgaagaag agattaacgt gccggatgat gaacggcgtt
    49561 aaaacccagt ttacacagca atgatgccgc actggcgtca tcacaaatta catacagaga
    49621 tgaccattca cggcgctcaa gatagttttt gcgcagttta tcgaactcac ccggtatccc
    49681 ggcgacttta cgcagcggtg cgtcatcgcg gcgcacatca tacaccaaat gcaccagcct
    49741 tttcagcgtc ggttgatcga gcggtccatg cagagtaatg cgaccaaact ctggcgcagg
    49801 cagtaatgta tccagcgcaa cgtgctgttc atgcccaata aacttgctat aggcttcaaa
    49861 cacttgcgta gtaccgcgtg ctttaccttc cagggtatag cctgcaatat gcggcgtgcc
    49921 gatatccact tttgtcagca attccacatt tagttccggt tcgccttccc agacatccag
    49981 cactacgctt aacttctggc cttcattcag gcaggtcagc agcgcggtat tatcgacgac
    50041 tgcgccacgg caggcgttaa tcagaatcgc tccgggcttc aggctacgga tcagtttttc
    50101 atccgccaga tgcagcgttt tgtacggccc gtctttgaag agtggcgtat ggaaagtcag
    50161 aatatccgcg cgctggacta actcatccag cgagcggaaa tcaccctcat ccccacagtc
    50221 ggcgcgaggc ggatcgcaaa gtaaggtttt aatccctaac gcttccagtc gtgcctgtaa
    50281 acgccgacca acgttaccaa cgcctacgat ccccacggta cgctcgtgca gtgaaaatcc
    50341 atcgcgttcg gcaagcatca gcagggagga gaaaacatat tccaccaccg caatcgcatt
    50401 acagccaggt gcagcggaaa aaccaattcc cgcctgcttc aaccatgctt catcgacatg
    50461 gtcggtcccc gcagtggcag tgccaacaaa tttaatgggt tttcctgcca gcaaagattc
    50521 attcactttc gtgaccgaac gcaccatcag cgcatccgcg tctgccagtt gagcgacggg
    50581 gattggacgc ccgggaaccg cgatcacctc acccaaacgg ctaaataagt cgcgggcata
    50641 aggcatattt tcatcaacaa ggattttcac gtttgtgtta cctgtatgag acgagagtta
    50701 accggacaag tgtgccataa tctcgcggcc aggcatactt gcgaagattt caggtataag
    50761 gatacgtaat gatacaacct atttccggcc ctcctcctgg gcaaccacca ggtcagggag
    50821 ataacctgcc gtctggcgcg ggcaatcagc ctttatccag tcagcaacgt acttcgctgg
    50881 aaagcttaat gacgaaagtg acctcactga cgcaacagca aagagcagaa ctgtgggcgg
    50941 gtatcaggca cgatattggt ctgtcgggag attcaccgct gctttcgcgt cactgccctg
    51001 ccgctgagca taatctggcg caacgtctgc tggccgcgca aaaaagccat tctgcccgcc
    51061 agcttttagc gcaattaggg gagtatttac gtctggggaa taatcgtcag gcggtcacgg
    51121 attatatccg tcataacttt ggtcagacgc cgctgaatca gctctcaccg gagcaattaa
    51181 aaaccattct caccctgttg caggaaggga agatggttat tccgcaaccg cagcagcgcg
    51241 aggcgaccga tcgtcctcta ttaccggcgg agcacaatgc gctcaaacag ctggtgacca
    51301 aacttgcggc ggcaacgggg gagcccagca aacagatctg gcaatcgatg ctggaacttt
    51361 ccggggtgaa agatggcgag ttaattccag cgaaactgtt taaccatctg gtgacctggc
    51421 tgcaggcgcg tcagacgcta agccagcaaa atacgccgac gctggaatca ctacaaatga
    51481 cgctaaaaca acctttagat gccagtgaac tggcggcgtt atcggcatat atccagcaaa
    51541 aatatggcct ttctgcgcaa tcatcgcttt cttctgccca ggccgaggat attcttaatc
    51601 agctttatca acggcgggtt aaagggattg atccgcgtga tatgcaaccg ctgcttaatc
    51661 cttttccacc gatgatggac acgttgcaaa atatggcaac gcgtcccgcg ctgtggatac
    51721 tgttagtcgc gattatcctg atgctggtct ggctggttcg ttaaccccga cgaaacgaca
    51781 gtatcgtgac aataattccc agcaccgcag agatcgcccc ggcaagaaat accgaagagt
    51841 aaccaaacgt ggtcgccagc attcccgcaa gcggcccgga gacgccgagg gcgatatcct
    51901 gaaacgcggc gtaaccgccc agtgcggtgc cgcgaacttg tgaggggacg cgtttaacca
    51961 cctccacgcc cagcgcagga aagataagcg aacatccggc tccggttaac gccgcgcccg
    52021 ctaatgcgac ccacgcccct ggggcttgcc agagcagcaa caagcccacc gtttctacaa
    52081 gcagagagac aatcgccact ttcacgccgc caaaacggtc cggcatccag ccaaacatga
    52141 cgcgcatcac gacaaatgcg ccgccaaacg cggtaagagt aaagcccgcc atcgcccatc
    52201 ctttgctggc aaagtagagc gaaacgaaag tcccgataac cgcaaaacca acgccttgta
    52261 gtgccagacc taaccctggt ttccagataa gtccgacaac gctccacaga gatggacgtt
    52321 ctcccgccag ggctggtact ttgcgcactg tgccgttaca ggcccacgcc agtaagggta
    52381 atgccattgt ggtgagcgcc agtgcggcaa aaccgtaatg gctatgaatc aacaggccaa
    52441 gcggagcacc aacagcgagg gcaccgtaaa tcgccattcc attccatgac atcactttgc
    52501 cagagtgttt tggccctacg atgcctaacc cccaggtcag agcgcctgtc agtaactggc
    52561 tttcaccaaa cccaagaatc aaacgcccga tgaccaacag ggcaaatttg aacggtgcgg
    52621 agacaggcaa aatcgccgcc agcagcaacg cgccgccagc cagaccacaa gctaacatcc
    52681 cctgaagcgc cgaacgtttt gcgccatatt gatcggccag tcgcccggca tagccacgcg
    52741 tcagcaccgt agccagaaac tgaatgccga cggcaatgcc gaccatggta ttgccatagc
    52801 ccagatcatg atgaacaaac aacgggataa ccggcaacgg caaccctacg gtcatgtagg
    52861 tgagaaaaac cgcaaaagcg atgcggaaga gcgaaaaatt ggcagaagat cgtgtttcgg
    52921 tttggcttac agcagtcatg cattactcca gaatgcagcg caaggcgagg agtatccccg
    52981 tctcatctct ctggtttcag ggttacagtg cgttggcagg atttaacgcg tacgtctttt
    53041 cagaaggaaa tcgacaaagc ggggagtttg cctggaactg gcggcgattg tcaatgatgt
    53101 gaaaaaggga accatcaggt tcccttttgc gttagtgccg gaggccgcat tggcgcgtaa
    53161 cgtcggatgc gacgctggcg cgtcttatcc gacctactcc ggcctactgc gaattaatct
    53221 ttcagcttgc gcattaccag cgtggcgttg gtgccgccga agccgaagct gttagacata
    53281 acagtggtca gttcgcgatc ggtcgtttcg gtcacgatgt tcagacccgc agcctgctcg
    53341 tccagctctt caatgttgat gctcggggcg ataaagccgt gttccagcat cagcagagag
    53401 tagatagctt cctgtacgcc agcagcgccc agagagtgac cggtcatggc tttggttgca
    53461 gaaatcgccg ggctcttatc gccgaacact tcacggatag ctgccagctc tttcacgtcg
    53521 ccaaccggag tcgaagtgcc gtgggagttc aggtaatcga ttggggtatc aacgccatgc
    53581 atcgccatct gcatacaacg tactgcgcct tcgccagagg gagcaaccat gtctgcgcca
    53641 tcagacgttg cgccgtagcc aacgatttca gcatagatgt gagcaccacg cgccagcgcg
    53701 tgttccagct cttcaaccac caccataccg ccgccgccag cgataacgaa accgtcacgg
    53761 tgagcatcgt aagtacggga ggctttttcc ggggtgtcgt tgtatttagt agacagcgca
    53821 cccattgcgt cgaattcgca agccatttcc cagcacagct cttcgccgcc gccagcaaac
    53881 acgatgtcct gtttgcccag ttggatctgc tctactgcgt taccgataca gtgtgcggaa
    53941 gtcgcacacg cggagctgat ggagtagtta acgccatgaa ttttaaacgg cgtggcgagg
    54001 caggcagaaa cgccggatgc catcgctttg gtgaccacat acgggccaac cgctttcagg
    54061 ccgcgcgggc cgcgcattgc gtcagcgccg aacacctgga aacgcgggga accgccgcca
    54121 gaacctgcaa tcaggccaac acgcggatta ttctggtaag cttccggaga gaggcccgca
    54181 tctgcgattg cctgctccat agaaaggaat gcataaatgg atgcgtcgct cataaagcgc
    54241 acaactttgc ggtcaatgag gccagtggta tccagtttta cgttgcccca gacgtggcta
    54301 cgcatgccgg aatccttcag ctcctgagag aaagtgatcc ctgaacgtcc ttcacgcaga
    54361 gatgccagga cttcctgctg gttattaccg atgctggaaa caatgcccag gccagtaatc
    54421 actgcacgtt tcattcaata cctctgtaag tcgcacatag agtaagtttc gaatgcacaa
    54481 tagcgtacac ttgtacgccg aacaagtccg atcagccaat taatagagaa atttgcgcag
    54541 ccttacacac atcgctaaga tcgagctacc gcctgtaaga cgagtaactt acgtgaaaca
    54601 ctactccata caacctgcca acctcgaatt taatgctgag ggtacacctg tttcccgaga
    54661 ttttgacgat gtctattttt ccaacgataa cgggctggaa gagacgcgtt atgtttttct
    54721 gggaggtaac caattagagg cacgctttcc tgaacatcca catcctctgt ttgtggtagc
    54781 agagagcggc ttcggcaccg gattaaactt cctgacgcta tggcaggcat ttgatcagtt
    54841 tcgcgaagcg catccgcaag cgcaattaca acgcttacat ttcattagtt ttgagaaatt
    54901 tcccctcacc cgtgcggatt tagccttagc gcatcaacac tggccggaac tggctccgtg
    54961 ggcagaacaa cttcaggcgc agtggccaat gcccttgccc ggttgccatc gtttattact
    55021 tgatgaaggc cgcgtgacgc tggatttatg gtttggcgat attaatgaac tgatcagcca
    55081 actggacgat tctctaaatc aaaaagtaga tgcctggttt ctggacggct ttgcgccagc
    55141 gaaaaacccg gatatgtgga cgcaaaatct gtttaacgcc atggcaaggt tggcgcgtcc
    55201 aggcggcacg ctggcgacat ttacgtctgc cggttttgtc cgccgcggtt tgcaggaagc
    55261 cggattcacg atgcaaaaac gtaagggctt tggacgcaaa cgggaaatgc tttgtggggt
    55321 gatggaacag acattaccac tcccctgctc cacgccgtgg tttaatcgta cgggcagcag
    55381 caaacgggaa gtagcgatta tcggcggtgg tattgccagc gcattgttgt cgctggcgct
    55441 attgcgtcgc ggctggcaag taacgcttta ttgcgcggat gaagcccccg cgctgggtgc
    55501 ttccggcaat cgccaggggg cgttgtatcc gttattaagc aaacacgatg aggcgctaaa
    55561 ccgctttttc tctaatgggt ttacttttgc tcgtcgactt tatgattcat tacccgttaa
    55621 atttgatcat gactggtgcg gcgtcacgca gttaggctgg gatgagaaaa gccagcataa
    55681 aatcgcacag atgttgtcaa tggatttacc cgaagaactg gctgtagccg ttgaggcaaa
    55741 tgcggttgaa caaattacgg gcgttacgac aaattgcagc ggtattactt atccgcaagg
    55801 tggctggctg tgtccggcag aactgacccg taatgtgctg gaactggcgc aacagcaggg
    55861 tttgcagatt tattatcaat atcagttaca ggatttatcc cgtaaggatg actgttggtt
    55921 gttgacgttt gcaggagatc agcaagcgac acacagtgta gtggtactgg cgaacgggca
    55981 tcaaatcagc cgtttcagcc aaacatcgtc tctgccagtg tattcggttg ccggtcaggt
    56041 cagccatatt ccgacgacac ccgaactggc aaaactgaaa caggtgctgt gctatgacgg
    56101 ttatctcacg ccgcaaaatc cggcgaatca gcaccattgt attggtgcca gttatcatcg
    56161 cggcagcgaa gagacggcgt acagtgaaga caatcagcag cagaatcgcc agcgattgat
    56221 tgattgtttc ccacacgcac aatgggcgaa gacggttgat gtcagtaaaa aagaggcgcg
    56281 ctgcggtgtg cgttgtgcca cccgcgatca tctgccaatg gtaggtaatg ttcccgatta
    56341 tgaggcaaca ctcgtggaat atgcgtcgct ggcggagcag aaagataagg cggtaagcgc
    56401 gccggttttt gacgatctct ttatgtttgc tgctttaggt tcgcgcggtt tgtgttctgc
    56461 cccgctgtgt gccgagattc tggcggcgca gatgagcgac gaaccgattc cgatggatgc
    56521 cagtacgctg gcggcgttaa atccgaatcg gttatgggtg cggaaattgt tgaaaggtaa
    56581 agcggttaag gccgggtaat ctgctctcgc agtgtttgcc ggatgcggcg tgaacgcctt
    56641 atccgaccta cgtgtgacgt gtaggcctga taagacgcga taagcgtcgc atcaggcatg
    56701 gtgctccaga cgccgcaact tactgctgtg acgcctgctg aaacaggttt tcccacatat
    56761 cggtcaccag cgcctggtca cgcggcgaca attcaccagc accaatggct ttttccagac
    56821 tctggctaac ggtcgtatgc accgcctgag cggagtggtc atcaccactt tccagttctg
    56881 cgatggctaa tgtaaggtgg ccacgcaaat acccactggc aaacaactca tcatcactgg
    56941 catggtcaac cataccgtcg attaatgcca gaatgcgtga ttcaaactcc gcgatcatct
    57001 tctttcctca ttgtaaaacg tggcgctggc ttcagttgag cgcttccggc cacgggaact
    57061 gttccgccgt aagttccggc gtgtgataat aattttgtag tgctttaatg aaacgtgccg
    57121 ggcgttccgg aatgccattt gttagataat ccatcacctg cgcatgtact cgccgttgaa
    57181 acaccacgcg gtccggttcg aagtcgcctt ccagattgtc gcaactgacg ttaaacgggt
    57241 atcccgccgc cacgcagaac aaccagtcga gcgcctgcgg cttcacttca acatcttcaa
    57301 actggctttg cgtttgggca tcgcgaccgt ccgggcaata ccagtagccg aaatcaacct
    57361 gctcgcggcg cgctttcccg gcaatacacc agtgcgaaat ctcatgaatt gcgctggcat
    57421 aaaagccgtg ggcaaagacg attcggttat acggtacttc cgcatcagcg ggaagataga
    57481 tcggttcgtc gtcgccttta atcagacggg tattaaaatc atcggcaaag cagctattaa
    57541 aaatttcaat caactgctca tagtggtgtg tactgttcat tagttcatcc ccaaccagtg
    57601 gaggatctcc tgtccgtggc tatcataaag taatttggca ctcatcaccg ccgagacgat
    57661 aacgatcatc gggcggatca gtttttgtcc tttgctcaac accagtcttg aacccatgcg
    57721 cgcgccaagg aactgcccca ccagcatcac aaagcccgtc gcccaaatca ctttgccgcc
    57781 gagaataaac agcagtaaac cgccgatgtt tgacgttgcg ttgagtaatt tggcgtgagc
    57841 cgtggctttg gcgaggttga atccgcacag cgtaacaaag gccagcgcgt aaaacgatcc
    57901 ggctgccgga ccaaagaatc cgtcataaaa accgacgcag ccaccggcaa ttaacgcgaa
    57961 gggcaaaccg tacatccggc gctggcgatc ttcttcaccc agctttggca tcagcaagaa
    58021 atagaggccg atacaaatca ccagaatggg caaaatctgc cgtaagacat cagcctgaac
    58081 gtactgtacc aacagtgcgc cgctcattga gccgacaaag gtcatggcaa tattaagttt
    58141 ctgatcgctt aaactaacca ctttgcggcg aataaagtag atagtggcgg aaatagagcc
    58201 gccgcaggct tgcagtttat tggttgccag agcattagcg ggagacatcc ccgctgccat
    58261 caatgccgga atggtgagta acccaccgcc accggcaatc gagtcgataa atcccgccag
    58321 catggcgaca aaaaagagaa ctcccagcaa cagcggggaa accataaaca ggctattaaa
    58381 cgtttccatt agatcacgtg ctcatccagt agcgcctggc aggaaggcgg caacggaggc
    58441 ggtgtcttct tctcaggctt tgttgttccc ggttttggag gttcaaacca gctttgcagt
    58501 tctgccccgc aaccatcgcc tggtggcggt aaaggttgat cttcacactc cagactatcg
    58561 gcaggacaac gtaatcgtac atgcatatgc gcacgatgct ggaaccaggg tcgcactttg
    58621 cgcaaccagt cgcgatcggt gcccgcatca aggcaaagtt gttgtttaat cgccggatta
    58681 acaaaaatgc gcgtgacgtc tttgtcctgg gcggcgagtt tgatcaagct gaaaatttct
    58741 ggcttccaca gcgtggagac aacgtgttta ccgtcgcggg aaactaagtc cagtgcttgc
    58801 gggcgcaaga gctgcgcgga ggtccggcgt gttttcggca gttgcagaaa gatatcgaca
    58861 tccagtccgg tctggtggct ggcgtgaccg ccgttgaaac gcccaccagc aggcattccc
    58921 atatcgccaa tcaacaccgt acccatgccc agattgctca cctgactact caggcgctgg
    58981 ataaacatca ccaggtccgg gtgaccgaaa tagcgacgct gatcggtacg catgacctga
    59041 taatgttcgg actgtatcgg caacgtgtca gcgccgacga tacaaccatt ggaaaaactg
    59101 cctatcgatt gtgcgctacc cggcacaggt tgggttattt tttgccacgg cgtcgctgcc
    59161 aggctgacgc tactggcaag cagggccagc agcgcaatcg cggttttgtt cattttttac
    59221 cagcgtggaa tatcagtctt cacatcggca ttttgcgccc gctgccgtaa caggtgatcc
    59281 attaaaacga tcgccagcat cgcttctgct atcggcaccg cgcggatccc gacacaggga
    59341 tcgtgacggc ctttggtgat catctcaact tcttcgccaa agcggttaat ggtgcgcccc
    59401 ggcacggtaa tactggaggt cggtttcagt gccatatgag caatgatttg ctgcccgctg
    59461 ctaattccgc cgagaatgcc gcccgcatgg ttactctgga aaccgtcttt ggtgatttcg
    59521 tcgcggttct ggctgccacg cagcgccacc acgtcaaaac catcaccaat ttccacgcct
    59581 ttcaccgcgt taatgctcat tagcgcatgg gcgatgtcgg catccaggcg atcaaagacc
    59641 ggctcaccaa gcccggcagg aacgccactg gcaacaacgg tgactttcgc gccaatagag
    59701 tcgccctctt ttttcagcgc gcgcatcagt tcatctaacg cgtcgatttt gtccgggtcc
    59761 gggcagaaga atggattttg ctcgacctgc gaccagtctt tgatttccag cggaatgtcg
    59821 cccatctggg tcaggcagcc gcgaatttcg atgccaaatt tctcagcgag atattttttg
    59881 gcaatcgccc ctgctgcaac gcgcatggcg gtttcacggg cggaagaacg tccaccgccg
    59941 cgataatcgc gcagaccgta tttttgttcg taggtgtaat cggcatggcc aggacggaaa
    60001 acgtccttaa tcgcactata atcctgagaa cgctgatcgg tattttcgat caacaaacca
    60061 atgctggtgc cggtagtaac accttcaaaa acaccggaga gaattttgac ctgatctggc
    60121 tcacgacgct gggtggtata gcgcgatgtc ccagggcgac gacggtcgag gtcatgttgc
    60181 aggtccgctt ccgtcagcgg aatgcctggc ggaacaccat cgacgatgca gccgagcgcc
    60241 agcccgtgcg attcgccgaa ggtggttacg cgaaagagtt gtccaattgt gtttccagcc
    60301 atcacggctc cgttattgtt gtgtttgcgt gtttacttaa tctttataaa tcgcgaaatg
    60361 ttcacgggcc gccagcagct gcgctttggt gagcataaac acaccatcgc cgccgttatc
    60421 aaactccagc caggtgaacg gaacatcggg atattgttcc ataagatgta ccatgctgtt
    60481 gccgacttca caaatcagta cgccatcatc agcaagataa tctgccgcgt ttccgagaat
    60541 gcgacgcgtc agtttcaggc cgtcggtgcc agatgccagg cccagttccg gctcgtggcg
    60601 gtattcattt ggcaggtcgg acatatcttc cgcatcgaca tacggcgggt tagtgacaat
    60661 caggtcgtac tgcactttcg gcaaatcacg gaacagatcg gaacgaatcg gtatgacgtt
    60721 gtggatcaga ccgtgttctt cgatgttctg ttcggcaacc gccagcgcgt cgggagagat
    60781 atctaccgca tcgacttccg cttccgggaa ggcataagca caggcaatgg cgatgcagcc
    60841 gctaccggta cacatatcta aaatatgctg cggttgcttg ctgataagtc cggcaaattt
    60901 attgttgatc aattcaccaa tcggcgagcg cggcaccagc acgcgttcat cgacgtaaaa
    60961 ttcatggccg cagaaccacg ctttgttggt caggtaagct accggaatgc gttcgttgac
    61021 gcggcggatc acgcgctcaa caatacgatg tttttcgctg gaggtcagac gcgcggtgcg
    61081 catatcttcc ggaatatcca gcggcaggta gagcgacggc aataccagct gtacggcttc
    61141 atcccacggg ttatcggtgc cgtgaccgta ccagatattt gccgcgctga agcggctcac
    61201 cgaccagcgc aacatgtcct gaatggtttg cagctcattt actgcttcat caacgaaaat
    61261 tttatccacg tattcctcca gggcatgatc gcaataattt cggcggctag tttgccatga
    61321 agatgacgat aaatcagcat tcacgcgcgg tgagtgagga aaaatacgtt taaaacgatc
    61381 gattgcgcta cgagtcgggt aaactgtagg aaaattagaa atagagacag ataaatgaaa
    61441 aagaaaacaa cacttagcga ggaggaccag gctctgtttc gccagttgat ggcggggact
    61501 cgcaagatta agcaggacac cattgtccat cggccacagc gtaaaaaagt cagcgaagtg
    61561 cccgtcaaac ggttgattca ggagcaggtt gacgccagtc actatttctc tgacgaattt
    61621 caaccgttat taaatgctga tggcccggtg aagtatgtac gccctggtgt agaccatttt
    61681 gaggcgaaaa agctgcgccg cggcgattat tcgccggaat tgtttttgga tttacacggt
    61741 ctgacgcagc tgcaggccaa gcaggaactg ggcgcgttga tcgccgcctg tcgccgcgaa
    61801 catgtgtttt gcgcctgcgt gatgcatggt cacgggaaac atatattgaa gcaacaaaca
    61861 ccgctgtggc tggcacaaca tccgcatgtc atggcgtttc accaggcacc aaaagagtat
    61921 ggtggtgatg ccgcattgct ggtgttgatt gaagtggaag agtggctacc gccggagttg
    61981 ccatgaatat tagggggcgg atgcgttttg ccatttcgcc ctgaatgctg gtatcgaatg
    62041 ccggatgcgg cgtaaacgcc tgatccggct tatcaaaatg tgcaaattca atacgttgtg
    62101 attcagttgt aggcatgata agacgcgtca gcgtcgcatc aggcatcaac gcacaactac
    62161 agttcattca gtacatactg gaaacggcat gtagaatcaa ttggctcacg gctactggta
    62221 ttcacctgta ccacaatgtc gtgtagcttt ctatccgtca atggttgacg tgagggggtg
    62281 actgtcacct ttgcattatc acaaccatac gaggtaaagg ttaataagtc agaaacagca
    62341 cgacaatatg cccctctgga atcaaaaata ctgccatcgc agagtttgcc gctttccatc
    62401 aggtaggtcg aaagcgtagt cgtgattgtc gaaccatcgg attgcaatcg cagcgcttta
    62461 ttatggctaa cataattatc actccaggat atatcaaacg aagcgacggg aattgtcggg
    62521 caaatagaat catgaactgt tgggcttgta tacgtaccaa atccaaatgc ctcagatact
    62581 gaataaaatg aggcaatcca cggacaacca taattatcag attctacagt gacgttaaaa
    62641 gtcatatatg cacagacaaa gacacgagta ttgataccag ggcaatttga ggtgtccgta
    62701 cgtctataat cagtcaaaac cagtgtcggt gccgggccag atccgctcca ggatattaac
    62761 ttgagggtgt cgccttttct tgcattggag ttgtattgtc cgtagcttac ttctcccggc
    62821 gtcactaccg tgacatagaa atataaactg ccggaagcag taacgttttt catcgcataa
    62881 taaattttag tactggaaaa gcttgcactc atgagtttat caccggcata gcactttggt
    62941 gtttctaaac tgagagcaag taatatcaga cttgtcaaaa atatcgtcca ttttttcatt
    63001 ttaaaaatac tcaaaagtta ccgtcattgt ggcggtgaaa tcacccgatg taacatttcg
    63061 tccatttacg gtctgtaacc aggcattaaa attgacactg ttatttccct gatttaatgg
    63121 aaatgaggca cctgtggtac tgttaatagg tacagccgcc cctccggcag tttcaatgcc
    63181 aatcccaaca cccgttgcag aagatgacga atcaatcgac agaaatcctg gtaagtcggt
    63241 atcttctgtt cccatcaagg tcaccttgac attaaacgcc gtggtgcttt tgcaatcttt
    63301 caactgaaaa actaacggta cgcgatctga ctgaccacgt tgcattaaat cgctggcagc
    63361 aattgtgggg aaatgaattt ctgcaagtaa gttgccattg attacaggag tacaggattt
    63421 accaagcaaa ttaccggtaa aatgcaggtt atcggcagct ggggccgttc cgctgaacaa
    63481 tatcagacca ctgataagcg cccacgcacg gttattcttc attggtaatc cacctgtaac
    63541 gtagcccatg cttcaaagtc tgactcgggt aagtcgacac cgctcttttt tacgggtact
    63601 gccgttagta ccggaaggga tgtttcattc accactaagg gcgtatgcag cttaaaatcg
    63661 ctcccggctt gtttgaggtg tattcctaat ccattgagat ccgtggctac ggccgaatcg
    63721 tcaaagtcac tctgcgtacc actccaggtc aacgtcattt ccatttgagg gtcacggaca
    63781 gcggtatcgc aggtaatggt atacggcacg acctgttcgt aattgctacc gttaatgtca
    63841 tcaatattca cgtcccgaaa cgtcaactct atattttgcc cattactaat agagcaattg
    63901 ggtggctgca ctaaataacc atgaaaagag acattcgaga gcgccataaa gctaaagctc
    63961 cacaacatta agagagagat ccgtttcatt gataatccac cacaattgtg gcactggcgc
    64021 taaattccgc cgccgttagc gatacaccgc tctgcacgac aggaaccgct tccagagcca
    64081 gtgcgccaga tgaaaagtta aacggtaacc atgcgccagg cgttaaatcc aaaatggagt
    64141 gatcgcttac tttctgaata cggataccaa aaccggcaat acccgtatcg attaccgttt
    64201 ctccgttaat tacggttgtg gttgcctgca tatttaagcg caggtcgtcc attgccagat
    64261 tgttgcacac aagcgagtag tcaatggggc gtttgtaact taccccatcg atcttggaaa
    64321 tgaagacatt accaaactcc acgctcgcac ccgttaccgt acagggcggc ggggcatcaa
    64381 cgagcacacg caagaccatc gcaaccgatt tactccccgc cattacaggc gtgctcacaa
    64441 tgctacacag caataaccac gcccaggccg aataagctcc tttcatgcta ttgctcctat
    64501 ccctggcctt tgctgttttg ctcttcgtcc acctgacagg tatttcccgt acagcggaag
    64561 aacagcggca aacgcgcacc ataatcattc acataggtca gcaccgggac gctatccatt
    64621 ttgactttta acggcacaga ggtttttggc gatatcacca tgggggaaaa tcccgctgcc
    64681 ggattgccat ttttctgcgc actggcgtta ctgataacca cgaagtacgg cgtcgggtta
    64741 ttgacggtaa actcctgacc actgagggtt aaggtcacct tatgctgcca cgggtttttc
    64801 atgctgacgt tttccagcgc ctttggtcgc cagaacaact taatgcgcgt ctgcagcgct
    64861 atctgtagcg tgttaggttt gttcgatttc ggcgggatct cacgcacgtt gaaatagaac
    64921 acgctctcgc ggtcggcagg cagcttgttg atatccggca tgccctgtac cttcacctga
    64981 ccgttcatca tggaatcgat gcgctgcact ggcggcagga ccgtcagtgg cgagctaatc
    65041 ctgttaccct gtccatcttc catccagctt tgtgccagat acggcatttt cgggtcgtta
    65101 ttgcgcagcg tgacgctgat tgatttatca ctttcgttaa aaatcaggcg tgtacggtcc
    65161 ggcgtgacag aggcctgtac tgacaggctg gcggcagtta tcagtaacgc cagcgtcgtt
    65221 gcggccgttc tggcagagca aaatagattc gtcatggatc attccttaat cacgttatta
    65281 attggctgat accgatactg gcgcttccgt tggcatcacc tgctgggtct gctcctggat
    65341 caccggctta atttcatgag gcatcgaagg agatatcgcc cctgtttgct ggcatggtaa
    65401 taacaggcca ttgaacagat cggctggcag cgggtcaggc agatgaatat cgcagtgggc
    65461 cacaccgccc catgaaacga tcatatgctc gccaggtttt acgcccgcga ggtagacgtt
    65521 gccgtcatcg tcaaccagac cgacgttctg cgcgctgtcg tttttcactt ccgcgccaaa
    65581 cggcggataa ctgccatctt gcagacgcag caccgccatc gctttttgcc cgctgatcac
    65641 cgagaactta cggtagccaa tggccccttc ggtaagcgtg ccctgcacca cggactgggt
    65701 ggcttcggcg ttttccggca ggttgtttag gtcgatatac gcctggttgc ggtagtagtt
    65761 gttgacgtct gccaccactg ccttaccgaa catattggtg taaaccgccg cgccatttcc
    65821 ttccaccgga acaccagcca caccgtcggc atcaatcagc agacgcgtac cgcccatatt
    65881 ctgggtacgg tggagcgcgc caccttgtgc ggtgagcgtc gcgccgccct gtaaggaaat
    65941 acccgccgag gtgtactgcc cttcatggta gttagcgctg agatcgacct gcgccagcga
    66001 tccgtcgtgg ctgtaataac cgtcaacgct ggagtgattg tcgctggtgc ctacgttcaa
    66061 ctggtaatgg gttgcgtcat cgacacggct gaaatacccc acctggctgc tgtccgaacc
    66121 gctgccgtag ttgccgttat agctgatggt gctgctgtca ccccacggca tactgagcga
    66181 tatgtacaca cctttatcgg cctggttgtc atactcgtag cggtatccgg tcatggaaat
    66241 gctcatgttg cggatactgc ccaggttgaa gtagtgcgag agcatgacgt tgtagttggt
    66301 ctgttcgtcg cgatcccagt aggtatggcg ggtgtagttg agataaacag acacaccggc
    66361 atccctgaag ttctggttat aggtggcggt gtacatctct ttgtcgttgc cggtgcgcac
    66421 catttcgctg tcgctggcat cgagatactc gctcatggtc atgaagttct cttccgagaa
    66481 gcggtatccg gcaaaagtga cgcggctgtt cagttcatcg aagtctttgg aatagctcag
    66541 gcgaaacgag ttgccgtcca gtgaaccttt cccgtaggcg gtctctttat ccagacgggt
    66601 atgcgagtgg gtgatatcaa aggccaccgc accaaacaca gacaggtcgc gaccgacgcc
    66661 aagcgccgcc gactgatagt gttcatctgc cagcgccccg ccgtatagcg accagccgtt
    66721 ggcaatcccc caggaagctt cgccgccgga gaagaaaccg ccttccacgt ggtgccccca
    66781 ctcctgcggg cggcccatca tcagtttata gcgcacctgg ccggggcgag tcaggaacgg
    66841 catcgaggcg gtgttgatgt catattcctg cacctgaccg ttctgttctt caatgcggat
    66901 atgcagcgta ccggagaccg aatcgccaag atcctggatg cggaacggcc ccgccgggac
    66961 ctgggtttcg tagatgacgc ggcccaactg gctgacggtc actttcgcgg tggtgtgcgc
    67021 cacgccggag atatccggcg catagccgcg cagattcggc ggcaacattt gatcgtcggt
    67081 gctgatactg ccacccacgt agttaaagcc gtcgaaaata tcagaattca ggtagtcttc
    67141 gccaaggcca agtttggctt ttagcgacgg taaggctcgc caggcgtagt agcggctcca
    67201 ctcccagttt ttttgcgtgt catcaccgtt gataacatcg tcatcattgc ttttactatg
    67261 caaataatca gtctgccagt cggcacgaag acgccatgcg ccgaggttca ccccaaccgt
    67321 cccgttaccg ctaatttcat tgctgtcatc cccgccattt tcttcatgtc gtgtctgggc
    67381 ggtaatactg tagtcagcaa ttaaaccaga tataccgtca tcccagcgtg aaggcggatc
    67441 ccagttgatg tcggtatatt caaggtaagc ctggggtaat gaaatgacta acgctgactg
    67501 actcaggtca gctttaatat caatgccttc cagttgaccg ggtttcaggc atttgccgtc
    67561 gtggatccat tgcaggtttt ttgccacatc ctcttttaag ccaaactgcg cgaccagttc
    67621 aggcgtcagg caggcatagg ttttactggc atcgttctca gaggcgtacc agtaaatatc
    67681 gtattcttcc gtcagcggct gtttatttag ttgaacctgt aaattgtatt tcccaggttc
    67741 gacataaccc tggctggaaa atcgcttcag atcgattttt gtgtcgcctt ttaactccag
    67801 aaaacgggaa tcaaactgaa tttcattttc agcccaggca ttgacataac tcccggacat
    67861 tgccaatgca acgcagcagg gaagtacccg cagtcggaaa agagaatggt caggcataca
    67921 cgtatccatc cgtaattatt ataatttgcg cgaattccat ttaaaaaccc catagcccga
    67981 aagctatggg ggtatcactt agagataagt aatctggaag gtcgtgttgg cttcaaaatt
    68041 acctaaatct ggcgcatcag cagcgcccac cagccaggct ttaaagtcca gcgtctgctt
    68101 cgctttgcct ttgttggtcg ccgtatcgtt tacgattttc tgttcgatac ccgcgccgct
    68161 tttataagag gttccctgag cgtcaccaat ggccaggctg acattgttaa atgccgcacc
    68221 agtatcggta ttgtaaatgg tgtagtaatt gccgctgttg gtagaagacg cgttaccggt
    68281 gaaggtagtg gtcatcgttg tctgggtgtc aaacacacag tcttgcagat ggatctggaa
    68341 agctttcggt gtagaagtcc cgttattttt caggatccct gaaccaatgt cacccatatc
    68401 aacttccagt ttgtgatcat caggaacgat agagcacggg gaatcttcaa tggtgccgta
    68461 gaaacgtgct gtaccattgt tgccagcggc gattgttgaa gtagaagcga acgcacccat
    68521 taccattgtt gcagcaatag ctgtttttac aaacttactc at
//
LOCUS       DAZKIF010000008        65367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZKIF010000008 DAZKIF010000000
VERSION     DAZKIF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.008"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(37..519)
                     /gene="folK"
                     /locus_tag="ACHOTW_000583"
     CDS             complement(37..519)
                     /gene="folK"
                     /locus_tag="ACHOTW_000583"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP9842753.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            complement(516..1913)
                     /gene="pcnB"
                     /locus_tag="ACHOTW_000584"
     CDS             complement(516..1913)
                     /gene="pcnB"
                     /locus_tag="ACHOTW_000584"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP9842754.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1973..2869)
                     /gene="gluQRS"
                     /locus_tag="ACHOTW_000585"
     CDS             complement(1973..2869)
                     /gene="gluQRS"
                     /locus_tag="ACHOTW_000585"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP9842755.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2936..3391)
                     /gene="dksA"
                     /locus_tag="ACHOTW_000586"
     CDS             complement(2936..3391)
                     /gene="dksA"
                     /locus_tag="ACHOTW_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP9842756.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3569..4273)
                     /gene="sfsA"
                     /locus_tag="ACHOTW_000587"
     CDS             complement(3569..4273)
                     /gene="sfsA"
                     /locus_tag="ACHOTW_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9842757.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4288..4818)
                     /gene="thpR"
                     /locus_tag="ACHOTW_000588"
     CDS             complement(4288..4818)
                     /gene="thpR"
                     /locus_tag="ACHOTW_000588"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP9842758.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            4892..7321
                     /gene="hrpB"
                     /locus_tag="ACHOTW_000589"
     CDS             4892..7321
                     /gene="hrpB"
                     /locus_tag="ACHOTW_000589"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP9842759.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSCVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7517..10051
                     /gene="mrcB"
                     /locus_tag="ACHOTW_000590"
     CDS             7517..10051
                     /gene="mrcB"
                     /locus_tag="ACHOTW_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP9842760.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10271..12514
                     /gene="fhuA"
                     /locus_tag="ACHOTW_000591"
     CDS             10271..12514
                     /gene="fhuA"
                     /locus_tag="ACHOTW_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP9842761.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVELKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12565..13362
                     /gene="fhuC"
                     /locus_tag="ACHOTW_000592"
     CDS             12565..13362
                     /gene="fhuC"
                     /locus_tag="ACHOTW_000592"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP9842762.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13362..14252
                     /gene="fhuD"
                     /locus_tag="ACHOTW_000593"
     CDS             13362..14252
                     /gene="fhuD"
                     /locus_tag="ACHOTW_000593"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP9842763.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14249..16231
                     /gene="fhuB"
                     /locus_tag="ACHOTW_000594"
     CDS             14249..16231
                     /gene="fhuB"
                     /locus_tag="ACHOTW_000594"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP9842764.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16266..17546)
                     /gene="hemL"
                     /locus_tag="ACHOTW_000595"
     CDS             complement(16266..17546)
                     /gene="hemL"
                     /locus_tag="ACHOTW_000595"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP9842765.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17712..17759
                     /gene="yadX"
                     /locus_tag="ACHOTW_000596"
     CDS             17712..17759
                     /gene="yadX"
                     /locus_tag="ACHOTW_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP9842766.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17771..19192
                     /gene="clcA"
                     /locus_tag="ACHOTW_000597"
     CDS             17771..19192
                     /gene="clcA"
                     /locus_tag="ACHOTW_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP9842767.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19216..19281
                     /gene="yadW"
                     /locus_tag="ACHOTW_000598"
     CDS             19216..19281
                     /gene="yadW"
                     /locus_tag="ACHOTW_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP9842768.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19274..19618
                     /gene="erpA"
                     /locus_tag="ACHOTW_000599"
     CDS             19274..19618
                     /gene="erpA"
                     /locus_tag="ACHOTW_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP9842769.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19665..20288)
                     /gene="yadS"
                     /locus_tag="ACHOTW_000600"
     CDS             complement(19665..20288)
                     /gene="yadS"
                     /locus_tag="ACHOTW_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP9842770.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20326..21126)
                     /gene="btuF"
                     /locus_tag="ACHOTW_000601"
     CDS             complement(20326..21126)
                     /gene="btuF"
                     /locus_tag="ACHOTW_000601"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP9842771.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21119..21817)
                     /gene="mtnN"
                     /locus_tag="ACHOTW_000602"
     CDS             complement(21119..21817)
                     /gene="mtnN"
                     /locus_tag="ACHOTW_000602"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9842772.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21901..23418
                     /gene="dgt"
                     /locus_tag="ACHOTW_000603"
     CDS             21901..23418
                     /gene="dgt"
                     /locus_tag="ACHOTW_000603"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP9842773.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23548..24972
                     /gene="degP"
                     /locus_tag="ACHOTW_000604"
     CDS             23548..24972
                     /gene="degP"
                     /locus_tag="ACHOTW_000604"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP9842774.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25127..26284
                     /gene="cdaR"
                     /locus_tag="ACHOTW_000605"
     CDS             25127..26284
                     /gene="cdaR"
                     /locus_tag="ACHOTW_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP9842775.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26373..26759)
                     /gene="yaeH"
                     /locus_tag="ACHOTW_000606"
     CDS             complement(26373..26759)
                     /gene="yaeH"
                     /locus_tag="ACHOTW_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP9842776.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27074..27898)
                     /gene="dapD"
                     /locus_tag="ACHOTW_000607"
     CDS             complement(27074..27898)
                     /gene="dapD"
                     /locus_tag="ACHOTW_000607"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP9842777.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27929..30601)
                     /gene="glnD"
                     /locus_tag="ACHOTW_000608"
     CDS             complement(27929..30601)
                     /gene="glnD"
                     /locus_tag="ACHOTW_000608"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP9842778.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30663..31457)
                     /gene="map"
                     /locus_tag="ACHOTW_000609"
     CDS             complement(30663..31457)
                     /gene="map"
                     /locus_tag="ACHOTW_000609"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9842779.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31825..32550
                     /gene="rpsB"
                     /locus_tag="ACHOTW_000610"
     CDS             31825..32550
                     /gene="rpsB"
                     /locus_tag="ACHOTW_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9842780.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32808..33659
                     /gene="tsf"
                     /locus_tag="ACHOTW_000611"
     CDS             32808..33659
                     /gene="tsf"
                     /locus_tag="ACHOTW_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9842781.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33806..34531
                     /gene="pyrH"
                     /locus_tag="ACHOTW_000612"
     CDS             33806..34531
                     /gene="pyrH"
                     /locus_tag="ACHOTW_000612"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9842782.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34681..35238
                     /gene="frr"
                     /locus_tag="ACHOTW_000613"
     CDS             34681..35238
                     /gene="frr"
                     /locus_tag="ACHOTW_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9842783.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35330..36526
                     /gene="ispC"
                     /locus_tag="ACHOTW_000614"
     CDS             35330..36526
                     /gene="ispC"
                     /locus_tag="ACHOTW_000614"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP9842784.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            36715..37473
                     /gene="ispU"
                     /locus_tag="ACHOTW_000615"
                     /gene_synonym="uppS"
     CDS             36715..37473
                     /gene="ispU"
                     /locus_tag="ACHOTW_000615"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP9842785.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37486..38343
                     /gene="cdsA"
                     /locus_tag="ACHOTW_000616"
     CDS             37486..38343
                     /gene="cdsA"
                     /locus_tag="ACHOTW_000616"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9842786.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38355..39707
                     /gene="rseP"
                     /locus_tag="ACHOTW_000617"
     CDS             38355..39707
                     /gene="rseP"
                     /locus_tag="ACHOTW_000617"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP9842787.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39737..42169
                     /gene="bamA"
                     /locus_tag="ACHOTW_000618"
     CDS             39737..42169
                     /gene="bamA"
                     /locus_tag="ACHOTW_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP9842788.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42290..42775
                     /gene="skp"
                     /locus_tag="ACHOTW_000619"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42290..42775
                     /gene="skp"
                     /locus_tag="ACHOTW_000619"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP9842789.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42779..43804
                     /gene="lpxD"
                     /locus_tag="ACHOTW_000620"
     CDS             42779..43804
                     /gene="lpxD"
                     /locus_tag="ACHOTW_000620"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP9842790.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43909..44364
                     /gene="fabZ"
                     /locus_tag="ACHOTW_000621"
     CDS             43909..44364
                     /gene="fabZ"
                     /locus_tag="ACHOTW_000621"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9842791.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44368..45156
                     /gene="lpxA"
                     /locus_tag="ACHOTW_000622"
     CDS             44368..45156
                     /gene="lpxA"
                     /locus_tag="ACHOTW_000622"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP9842792.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45156..46304
                     /gene="lpxB"
                     /locus_tag="ACHOTW_000623"
     CDS             45156..46304
                     /gene="lpxB"
                     /locus_tag="ACHOTW_000623"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP9842793.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46301..46897
                     /gene="rnhB"
                     /locus_tag="ACHOTW_000624"
     CDS             46301..46897
                     /gene="rnhB"
                     /locus_tag="ACHOTW_000624"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9842794.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46934..50416
                     /gene="dnaE"
                     /locus_tag="ACHOTW_000625"
     CDS             46934..50416
                     /gene="dnaE"
                     /locus_tag="ACHOTW_000625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9842795.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50429..51388
                     /gene="accA"
                     /locus_tag="ACHOTW_000626"
     CDS             50429..51388
                     /gene="accA"
                     /locus_tag="ACHOTW_000626"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9842796.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51487..53628
                     /gene="ldcC"
                     /locus_tag="ACHOTW_000627"
     CDS             51487..53628
                     /gene="ldcC"
                     /locus_tag="ACHOTW_000627"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP9842797.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQVSLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53685..54074
                     /gene="yaeR"
                     /locus_tag="ACHOTW_000628"
     CDS             53685..54074
                     /gene="yaeR"
                     /locus_tag="ACHOTW_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9842798.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54139..55434
                     /gene="tilS"
                     /locus_tag="ACHOTW_000629"
     CDS             54139..55434
                     /gene="tilS"
                     /locus_tag="ACHOTW_000629"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9842799.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(55487..55747)
                     /gene="rof"
                     /locus_tag="ACHOTW_000630"
     CDS             complement(55487..55747)
                     /gene="rof"
                     /locus_tag="ACHOTW_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP9842800.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55734..55934)
                     /gene="yaeP"
                     /locus_tag="ACHOTW_000631"
     CDS             complement(55734..55934)
                     /gene="yaeP"
                     /locus_tag="ACHOTW_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP9842801.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56100..56645
                     /gene="yaeQ"
                     /locus_tag="ACHOTW_000632"
                     /pseudo
     CDS             56100..56645
                     /gene="yaeQ"
                     /locus_tag="ACHOTW_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
     gene            56642..57052
                     /gene="arfB"
                     /locus_tag="ACHOTW_000633"
     CDS             56642..57052
                     /gene="arfB"
                     /locus_tag="ACHOTW_000633"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP9842802.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            57066..57776
                     /gene="nlpE"
                     /locus_tag="ACHOTW_000634"
                     /gene_synonym="cutF"
     CDS             57066..57776
                     /gene="nlpE"
                     /locus_tag="ACHOTW_000634"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP9842803.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57976..58800)
                     /gene="yaeF"
                     /locus_tag="ACHOTW_000635"
     CDS             complement(57976..58800)
                     /gene="yaeF"
                     /locus_tag="ACHOTW_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFP9842804.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58853..60571)
                     /gene="proS"
                     /locus_tag="ACHOTW_000636"
     CDS             complement(58853..60571)
                     /gene="proS"
                     /locus_tag="ACHOTW_000636"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9842805.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60682..61389)
                     /gene="tsaA"
                     /locus_tag="ACHOTW_000637"
     CDS             complement(60682..61389)
                     /gene="tsaA"
                     /locus_tag="ACHOTW_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP9842806.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61386..61790)
                     /gene="rcsF"
                     /locus_tag="ACHOTW_000638"
     CDS             complement(61386..61790)
                     /gene="rcsF"
                     /locus_tag="ACHOTW_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP9842807.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            complement(61908..62723)
                     /gene="metQ"
                     /locus_tag="ACHOTW_000639"
     CDS             complement(61908..62723)
                     /gene="metQ"
                     /locus_tag="ACHOTW_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP9842808.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62763..63416)
                     /gene="metI"
                     /locus_tag="ACHOTW_000640"
     CDS             complement(62763..63416)
                     /gene="metI"
                     /locus_tag="ACHOTW_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP9842809.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63409..64440)
                     /gene="metN"
                     /locus_tag="ACHOTW_000641"
     CDS             complement(63409..64440)
                     /gene="metN"
                     /locus_tag="ACHOTW_000641"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP9842810.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64628..65203
                     /gene="gmhB"
                     /locus_tag="ACHOTW_000642"
     CDS             64628..65203
                     /gene="gmhB"
                     /locus_tag="ACHOTW_000642"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP9842811.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLSQAIKKQQKPAQ"
BASE COUNT        15412 a        16249 c        18051 g        15655 t
ORIGIN      
        1 aagtgaggag taagataaga aaataaaata atacagctaa taccagatgg caggttgtag
       61 tactgctagc gaagtgagta tttcacgtaa tgtctcccca tcaggaaaca cgagttccgg
      121 ggcaatctca aacagcggcc acagcataaa gccgcgattc ttcatatcgt aatgcgggac
      181 ggtgagtcgt tcggtattaa tggtttcatt accaaacaac atgatatcaa gatcgagtgt
      241 gcgcggtccc cagcgttctg ctttacgaac gcgaccttgc tgcaactcta tttgctgtgt
      301 gtgattaagt aattcttcag gcgcaagtgt tgtttccagc gccacggctg cgtttaagta
      361 atcgggttga tcttgcggcc ccagcggtgg ggtgcggtaa aacgaagaaa cggtaagaat
      421 gtggctttca gggatatcgc ctaatgcttt cagggcagca ttgacctgct ccagcggaga
      481 ggccagattg ctgcctatgg caatatacgc cactgtcatg cggtaccctc acgacgtggt
      541 gcgcgtttgc gtggacgacg agtacgacga cgcggtgacg gttcttcatc cagctcgttg
      601 agcatccctt tttggtctgg tggcgcggaa acctggaact caccccacca tttcaccaga
      661 cgctgcagtt cagcgttacg ctcaacttca gctcgcaagg ccaacaggtc ataagccgca
      721 cggaacttag gatgctccag caatttccat gcgcgtttac cctgacgtcg ggacatacgc
      781 aactgcaact gccagatatc gcgggttaac gtcgtcagac gtttcgggat tgccagtgaa
      841 cggcaggctt cgtccagcac gtcgttcatc gccagcgcga aagcgtcgtg ataggtcagg
      901 ccgctttcct gggcgatctt ctgtgccgtc tccagcagtg ggtaccagaa catggcggca
      961 aacaggaacg ccgggttcac gcgcatatcg ttatggatac gcgtatcggt attcttcagc
     1021 acctgttcaa tgatccgctc catcgggctg tcgccatttt ccgtgaagta gcgggtaatg
     1081 gtcgggaaca gcggctggaa cagatgatat tcacacaaca gcttataggt ttcgtaaccg
     1141 tagcccgctt gtagcagttt aagcgattct tcaaacaggc gtgccggtgg gatatcgttc
     1201 agcagggtag cgaggcgagg gatcggttct gcggtttccg ggctgatgcg catacccaat
     1261 ttggcggcaa aacgtactgc gcgcagcata cgtaccggat cttcacggta gcgcgtttcc
     1321 gggttaccaa tcagacggat aacaccgtcc ttcagatcct tcataccgcc aacgtaatca
     1381 cggacggtaa aatccgctac gctgtaatac aggctgttga tagtgaaatc gcggcgctgg
     1441 gcgtcttctt cgatggagcc gaaaatgttg tcgcgcagca acatgccgtt ttgcccgcgt
     1501 tgggaggtcg tgcggtcgct gacgttacct tcgtggtgtc cacggaaggt cgcaacttcg
     1561 ataatctccg ggccaaacat cacatgagcc agacggaaac ggcgacccac caggcggcag
     1621 ttacggaaca gtttgcgcac ctgctcaggc gtggcgttag tggttacgtc aaaatctttc
     1681 ggctttttgc cgagtaacag gtcgcgcacg ccgccgccaa ccagccaggc ttcgtatccc
     1741 gctttattga gcctgtacat taccttcagg gcattttcac tgatatcttt gcgggaaata
     1801 gcatgctgct cacgcgggat caccgtcacc tgtggacggg cgactgcctg ttcagcctcg
     1861 ctttcctcgc ggcttagcac cttgcggcaa aaattagcga ctcgggtaaa aatagtacac
     1921 ctcggtagtg tcaaacatca ttcaggacaa aaaaatagcg gctaatcata gctcagcatg
     1981 acgcatttga gaatgttgaa tttacaattg ccgactcagg cactgcggta agccgccagt
     2041 ttttgacggc tgactgaagg atttgctcga cgctgaaatc ctgccagtgt gcttctgcct
     2101 gctgccccag aaattgaagt gccgcgatta gtaccgggcg tggatcgcct ttcggcaacg
     2161 caggcgcatg attctgcttg gaaagtttag cgccttgtgg attaagcgcc agcggcagat
     2221 gaatgtaatc tggcactttc cagccaaaaa gctggtacag cgagatttgc cgcactgtcg
     2281 gttcgatcaa atctgccccg cggacaattt cgctcacgcc ctggaagtga tcatccacca
     2341 ccaccgccag gttataggca aacaaaccat cacggcgatg aataataaaa tcttcccgtg
     2401 ccagtttttc gtcggcgtga ataatgccgc gcagctggtc agtaaattgc gtgaccggat
     2461 gctgctggcg gatacgcact gcggcgttgt ctggtccatg atgcaacacc cggcaatgac
     2521 cgtcgtaaat accgccaatg ctttgaatac gcgcacgcgt gcaggtgcag taataactta
     2581 gtccttgttc atgtaaccag gcgagtgctt cacgataggc gtggtgacgt tgcgattgcc
     2641 agagaacatc gccgtcccag tgcagaccgt aatgttccag ctggcgcagg atagtttctg
     2701 cggcaccggg aacttcacga ggcgggtcga tatcttcaat gcgtaccagc cagcgaccgt
     2761 gccgggcgcg agcctgcaaa tagctgccaa gcgcagcaat cagagaacca aaatgaagct
     2821 cgccggaagg agagggggcg aagcggccaa tatactgtgt gtctgtcatc tctttgaaca
     2881 aaaaataagg cgggagcatt tcccgcctgt ggtaaacgtg atggaacggc tgtaattagc
     2941 cagccatctg tttttcgcga atttcagcca gcgttttgca gtcgatgcac agatcggctg
     3001 tcgggcgcgc ttccagacgg cgaataccaa tttcaacacc gcaggattca cagtagccga
     3061 aatcttcgtc ttccactttt ttcagcgtct tctcgatctt tttgatcagc ttacgctcgc
     3121 gatcgcggtt acgcagttcg aggctgaact cttcttcctg ggctgcacgg tctaccgggt
     3181 ccgggaaatt ggctgcttca tcctgcatat gtgtaacggt gcgatcgact tcatccctga
     3241 gttgattacg ccatgcttcc agaatacgac ggaagtgcgc cagctgggct tcattcatat
     3301 actcttcgcc cggcttctcc tgatatggtt ccaccccagc gatggcgaga atactcaggg
     3361 acgatgtttt acggttttgc ccttcttgca tgttgcttct ccttaacacg cactatcgat
     3421 ccccatgttc gggggaaaaa tgaggccgct ataaatagca gatgcttttc cggatagcaa
     3481 ttatctaaac gtaacacttg acaactgtgt gaggaaaagc gtatttgcgc acgcgaccag
     3541 aatgtaaatt aaccagttac ttactttact acaatgtaac cggcagtgat tttttaagag
     3601 ccatgccttc agcagaaatt tccgctttgt aagccagaat ttctaccccc ctctgttgag
     3661 cttctgacaa tagttgcgcg tatttctcat cgatgtggcg cgcgggtgaa aaccgtgtaa
     3721 tggctgaatg cagcacggcg aaaaagataa ccgcacgctg gccttcagcc gctacgctca
     3781 tcaactcccg aaggtgtttc tgacctcgtt cagtgaccgc atcgggaaaa tatccctgtt
     3841 cgttctccgc taacgtaacc gatttcactt caatatagca gtctggacgc gaatccgcct
     3901 gcaacataaa gtcaatacgg ctgcgttctg cgccgtattt tacttcgctt ttcagcgagc
     3961 tatagcctga cagttctgaa attgattcat taaggatagc ctctttcgtc aacctgttag
     4021 cccaaagcgt gttgacgcaa ataaatgcgc cgctctggct ttgagttaat tcccaggtgt
     4081 gtgggtattt ccgtttggtg ttgtctgaag tcgaatacca gacggtatcg ccaggcgttg
     4141 cacaaccggt catcgcaccc gtattcgggc agtgtagcgt taattcgcga ccatcgggtg
     4201 tgatcacatc ggctaaaaaa cgtttgtaac gctgaattag cgtcgcgcgc tgtagagggg
     4261 gagaaaattc cattgcgaca atccttgtta ttgcgttagc acccagcgtt ttagcggcgt
     4321 gtagcgtgtg cgtccacggg caaacgagga ggcgtaaagg gtgaactccg tcaccgcata
     4381 tgaccagtta aaacctggcg gcgggattgt caccgcctcg ctggcgtcgc gcaataaggt
     4441 aatatgtgga tgaaacggac gattgctttg aaaacaaccg ctgcgggcag cctgtgaacg
     4501 gagcatattc gccagctgga ttaagccgcg tggcggctga cgcatcccta accacaccac
     4561 acgcgaacgc agccattggc cggcgtcatc aagcgtgagt gtgaaaccag gttgacgaat
     4621 ccgtccggct aaaagagaaa gcgccttctc tttctctgcg ctcacttcgc ctaaaaatgc
     4681 cagagtcaga tgcaaattat cggcggcgac cggacgtccc gcctcaagtg ggaagtgtgt
     4741 ggcgcgccag tggataatct gttcgcggat ttctgcaggt aagtcgatag caaagaacag
     4801 acgttgcggg tcagacatgt gaggcactcg gttatgaatt accgcgatgc tacaatgtgg
     4861 cgcgaagaat gttaaccctc tggagcgttt tgtgtcgtcg ttgcccgttg ctgccgtgtt
     4921 acctgaatta cttaccgctc tcgattatgc gccgcaggta ttattaagtg cgccgaccgg
     4981 ggccgggaaa tcaacctggc tgccgctgca actgctggcg catcccggca ttaacgggaa
     5041 aattatcctc ctggagccac ggcgtctggc ggcgcgtaac gtcgcgcaac ggctggcgga
     5101 gctgcttaac gaaaagccag gcgataccgt tggctaccgg atgcgtgcgc aaaactgcgt
     5161 cgggccgaat acccgcctgg aagtagttac cgaaggcgtg ctgacgcgga tgatccagcg
     5221 tgacccggaa ctgagctgtg ttggattggt gatccttgat gaatttcatg agcgtagctt
     5281 gcaggcggat ttggcgttgg cgctgttact cgatgtgcaa caaggtctgc gtgatgacct
     5341 taaactgctg attatgtcgg ctacgctgga caacgaccgc ttgcagcaaa tgctgccaga
     5401 agcgcccgtc gtcatctcag aagggcgctc gtttccggtt gaacgccgtt atttaccgct
     5461 gcccacgcat cagcgttttg acgatgccgt tgcggtagcc accgctgaaa tgctgcgtca
     5521 ggaaagcgga tcattactgt tatttttacc tggcgtcgga gaaattcagc gtgtgcagga
     5581 acaactggct tcgcgcatcg gcagtgatgt attgctctgc ccgctgtatg gcgcgttgtc
     5641 gctgaacgat cagcgaaaag cgatcctccc ggcaccgcaa gggatgcgca aagtggtgct
     5701 ggcgaccaat attgctgaaa ccagtttaac cattgaaggt attcgtctgg tggtggattg
     5761 tgcccaggaa agggtggcgc gttttgatcc gcgaacaggg cttacgcggt tgattactca
     5821 gcgaatcagt caggcgtcga tgacgcagcg tgccgggcgc gccgggcgtc tggagccggg
     5881 tatctgcctg catttaatcg ccaaagaaca agcagaacgc gctaccgcgc aaagtgaacc
     5941 ggagatctta caaagcgatc tttccggttt gctgatggaa ttactgcaat ggggatgcag
     6001 cgatccggcg cagatgagct ggctggatca accgccaacg gtgaatctac tggccgcgaa
     6061 acgtctgtta caaatgctgg gggcgctgga cggtgaacgg cttagtgcgc aagggcaaaa
     6121 aatggcagcg ctgggtaacg atccgcgttt agcggcaatg ctggttagcg caaagagcga
     6181 cgacgaagct gctaccgcgg caaaaattgc cgccattctc gaagagccgc cacggatggg
     6241 caatagtgac ctgggcgtgg cgttttcgcg caatcaacca gcctggcagc aacgtagtca
     6301 gcaactgtta aaacgcttaa acgtacgcgg cggtgaggca gacagttcgc ttatcgcgcc
     6361 gctacttgcc ggggcgtttg ccgatcgcat tgctcgccgc cgtgggcaag atggacgcta
     6421 tcaactggca aacggcatgg gggcgatgct tgatgccgac gacgcgctaa gtcgccacga
     6481 atggttgatc gcgccgttat tattgcaggg cagcgcctcg ccggatgcgc ggattttact
     6541 ggcgttaccg gtcgatattg atgaattagt acaacgctgt ccgcagctgg tgcagcaatc
     6601 tgacaccgtg gaatgggatg acgcgcaagg tacgctgaaa gcctggcgtc ggctgcaaat
     6661 cggtcagttg acggtgaaag tgcagccgtt ggcgaaaccg tcagaagacg agttgcatca
     6721 ggcgatgctt aacggcatcc gtgataaagg tttaagcgtg ctcaactgga cggcggaagc
     6781 ggaacagcta cgcttgcgtt tgttatgcgc cggaaagtgg ttgccagaat atgactggcc
     6841 agcggttgat gatgaaagtt tgttggcgac gctggaaacg tggctgctgc cacatatggc
     6901 cggtgtacat tcactacgcg gcctgaaatc actcgacatt tatcaggcac tacgcggatt
     6961 acttgattgg ggaatgcagc aacgtctgga tagtgaattg cctgcgcatt acactgtgcc
     7021 gacgggaagc cggatcgcca ttcgttatca tgaagataac ccgcccgcgc tggcggtgag
     7081 aatgcaggag atgtttggtg aggccactaa tccgacgatc gcccaggggc gcgtgccgct
     7141 ggtgctggaa ttgctttcac ccgcccaaag gccgctgcaa atcacgcgtg atttgggggc
     7201 cttctggaaa ggggcgtatc gtgaggtgca aaaggagatg aaagggcgtt atcccaaaca
     7261 cgtctggccg gacgacccgg caaacacggc accgacgcga cggacgaaaa agtattcgta
     7321 aggttggttt tctccctctc cctgtgggag agggccgggg tgagggcacc agcgcgcacg
     7381 ttcaccctca ccctaaccct ctcccttaag ggagagggga ccgatcgagc tcaaattttg
     7441 agagatatct tcttctgtct tgtaacagaa gaacagaaaa tcgggctttt gcgcctgaat
     7501 attgcggaga aaaagcatgg ccgggaatga ccgcgagcca attggacgca aagggaaacc
     7561 gacgcgtccg gtcaaacaaa aggtaagccg tcgtcgttac gaagatgacg atgattacga
     7621 cgattatgat gactatgagg atgaagaacc gatgccgcgc aaaggtaagg gcaaaggcaa
     7681 agggcgtaag cctcgtggca aacgcggctg gctatggctg ctgttaaaac tggctatcgt
     7741 ttttgccgtg ctgatcgcca tttacggcgt ttatctcgat caaaaaattc gtagccgtat
     7801 tgatggcaag gtctggcaac tgcctgcggc agtttatggt cgaatggtca accttgagcc
     7861 agacatgacc atcagcaaga acgagatggt gaagctgctg gaggcaacac agtatcgtca
     7921 ggtgtcgaaa atgacccgtc ctggcgaatt taccgtgcag gccaacagca ttgagatgat
     7981 tcgccgtccg tttgatttcc cggacagtaa agaagggcag gtgcgcgcgc gtctgacctt
     8041 tgatggcgat catctggcga ccatcgtcaa tatggagaac aaccgtcagt tcggtttctt
     8101 ccgtcttgat ccgcgtctga tcaccatgat ctcctcacca aacggtgagc agcgtttgtt
     8161 tgtgccgcgc agtggtttcc cggatttgct ggtggatact ttgctggcga cagaagaccg
     8221 ccatttctac gagcatgatg gaatcagtct ctactccatc ggacgtgcgg tgctggcaaa
     8281 cctgaccgca ggtcgcacgg tgcagggggc gagtacgctg acgcaacagc tggtgaaaaa
     8341 cctgttcctc tccagcgagc gctcttattg gcgtaaagcg aatgaagcct acatggcgct
     8401 gatcatggac gcgcgttaca gcaaagaccg tattcttgag ctgtatatga acgaggtgta
     8461 tctcggtcag agcggcgaca acgaaatccg cggcttcccg ctggcgagct tgtattactt
     8521 tggtcgcccg gtagaagaat tgagcctcga tcagcaggcg ctgttagtcg gtatggtgaa
     8581 aggagcgtcc atctacaacc cgtggcgtaa cccgaaactg gcgctggagc gacgtaatct
     8641 ggtactgcgt ctgctgcaac agcaacagat tattgatcaa gaactctatg acatgttgag
     8701 tgcgcgtccg ctgggggttc agccgcgcgg tggggtgatc tctcctcagc cagcctttat
     8761 gcaactggtg cgtcaggagc ttcaggctaa attaggcgat aaggtaaaag atctctccgg
     8821 cgtgaagatc ttcactacct ttgactcggt ggcccaggac gcggcagaaa aagccgccgt
     8881 ggaaggtatt ccggcactga agaaacagcg taagttgagc gatcttgaaa ctgcgattgt
     8941 ggtcgtcgac cgctttagtg gtgaagttcg tgcgatggtc ggaggttctg agccgcagtt
     9001 tgcgggctac aaccgtgcga tgcaggcgcg tcgttcgatt ggttcccttg caaaaccagc
     9061 gacttatctg acggccttaa gccagccgaa aatctatcgt ctgaatacgt ggattgcgga
     9121 tgcgccaatt gcgctgcgtc agccgaatgg ccaggtctgg tcaccgcaga atgatgaccg
     9181 tcgttatagc gaaagcggca gagtgatgct ggtggatgcg ttgacccgtt cgatgaacgt
     9241 gccgacggta aatctgggga tggcgctggg gctgcctgcg gttacggata cctggattaa
     9301 actgggcgta ccgaaagatc agttgcatcc ggttccggca atgctgctgg gggcgttgaa
     9361 cttaacgcca atcgaagtgg cgcaggcatt ccagaccatc gccagcggtg gtaaccgtgc
     9421 accgctttct gcgctgcgtt cggtaatcgc ggaagatggc aaagtgctgt atcagagctt
     9481 cccgcaggcg gaacgcgctg ttccggcgca ggcggcgtat ctgacactat ggaccatgca
     9541 gcaggtggta caacgcggta cgggtcgtca gcttggggcg aaatacccga acctgcatct
     9601 ggcagggaaa acagggacta ccaacaataa cgtagatacc tggtttgcgg gcattgacgg
     9661 cagcacggtg accatcacct gggtcggccg tgataacaac cagccgacca aactgtatgg
     9721 agccagcggg gcaatgtcga tttatcagcg ttatctggct aaccagacgc caacgccgct
     9781 gaatcttgtt ccgccagaag atattgcaga tatgggcgtg gactacgacg gcaactttgt
     9841 ttgcagcggt ggcatgcgtg tcttgccggt ctggaccagc gatccgcaat cgctgtgcca
     9901 gcagagtgag atgcagcagc agccgtcagg caatccgttt gatcagtctt ctcagccgca
     9961 gcaacagccg caacagcaac ctgctcagca agagcagaaa gacagcgacg gtgtagccgg
    10021 ttggattaag gatatgtttg gtagtaatta acatctaagc gtgaaatacc ggatggcgag
    10081 ttgccatccg gtaaaataac atcccaccca aagtattaac cctttctttt catctggttg
    10141 tttattaacc cttcaggaac gctcagattg cgtaccgctt gcgaacccgc cagagtttcg
    10201 aatattatct tatctttata ataatcattc tcgtttacgt tatcattcac tttacatcag
    10261 agatatacca atggcgcgtt ccaaaactgc tcagccaaaa cactcactgc gtaaaatcgc
    10321 agttgtagta gccacagcgg ttagcggcat gtctgtttat gcacaggcag cggttgaact
    10381 gaaagaagac actatcaccg ttaccgctgc acctgcgccg caagaaagcg catgggggcc
    10441 tgctgcaact attgcggcgc gacagtcagc taccggcact aaaaccgata cgccgattca
    10501 aaaagtgcca cagtctattt ctgttgtgac cgccgaagag atggcgctgc atcagccgaa
    10561 gtcggtaaaa gaagcgctta gctacacgcc gggtgtctct gttggtacgc gtggcgcatc
    10621 caacacctat gaccacctga tcattcgcgg ttttgcggca gaaggccaaa gccagaataa
    10681 ctatctgaat ggcctgaagt tgcagggcaa cttctataac gatgcggtca ttgatccgta
    10741 tatgctggaa cgcgctgaaa ttatgcgtgg cccggtttcc gtgctttacg gtaaaagcag
    10801 tcctggcggc ctgttgaata tggtcagcaa gcgtccgacc accgaaccgc tgaaagaagt
    10861 tcagtttaaa gccggtactg acagcctgtt ccagactggt tttgacttta gcgatgcgct
    10921 ggatgatgac ggcgtttact cttatcgcct gaccggtctt gcgcgttctg ccaatgccca
    10981 gcagaaaggg tcagaagagc agcgttatgc tattgcaccg gcgttcacct ggcgtccgga
    11041 tgataaaacc aatttcacct tcctttctta cttccagaac gagccggaaa ccggttatta
    11101 cggctggttg ccgaaagagg gaaccgttga gccgctgccg aacggtaagc gtctgccgac
    11161 agactttaac gaaggggcga agaacaacac ctattctcgt aatgagaaga tggtgggcta
    11221 cagcttcgat cacgaattta acgacacctt tactgtgcgt cagaacctgc gctttgctga
    11281 aaacaaaacc tcgcaaaaca gcgtttatgg ttacggcgtc tgctccgatc cggcgaatgc
    11341 ttacagcaaa cagtgtgcgg cattagcgcc agcggataaa ggccattatc tggcacgtaa
    11401 atacgtcgtt gatgatgaga agctgcaaaa cttctccgtt gatacccagt tgcagagcaa
    11461 gtttgccact ggcgatatcg accacaccct gctgaccggt gtcgacttta tgcgtatgcg
    11521 taatgacatc aacgcctggt ttggttacga cgactccgta ccgctgctcg atctgtacaa
    11581 tccggtgaat accgatttcg acttcaatgc caaagatccg gcaaactccg gcccttaccg
    11641 cattctgaat aagcagaaac aaacgggcgt ttatgttcag gatcaggcgc agtgggataa
    11701 agtgctggtc accctgggcg gtcgttatga ctgggcagat caagaatctc ttaaccgcgt
    11761 tgccgggacg accgataaac gtgatgacaa acagtttacc tggcgtggtg gtgttaacta
    11821 cctgtttgat aatggcgtaa caccttactt tagctatagc gaatcgtttg aaccttcttc
    11881 gcaagttggg aaggatggta atattttcgc accgtctaaa ggtaagcagt atgaagtcgg
    11941 cgtgaaatat gtaccggaag atcgtccgat tgtagttact ggtgccgtgt ataatctcac
    12001 taaaaccaac aacctgatgg cggaccctga gggttccttc ttctcggttg aaggtggcga
    12061 gatccgcgca cgtggcgtag aaatcgaagc gaaagcggcg ctgtcggcga gtgttaacgt
    12121 agtcggttct tatacttaca ccgatgcgga atacaccacc gatactacct ataaaggcaa
    12181 tacgcctgca caggtgccaa aacacatggc ttcgctgtgg gctgactata ccttctttga
    12241 cggtccgctt tcaggtctga cgctgggcac cggtggtcgt tatactggct ccagctatgg
    12301 tgatccggct aactccttta aagtgggaag ttatacggtc gtggatgcgt tagtgcgtta
    12361 tgatctggcg cgagtcggca tggcgggctc caacgtggcg ctgcatgtta acaacctgtt
    12421 cgatcgtgaa tacgtcgcca gctgctttaa cacttatggc tgcttctggg gcgcagaacg
    12481 tcaggtcgtt gcaaccgcaa ccttccgttt ctaatttctc ttttggggca cggatttccg
    12541 tgcctgtttc acaagttggc tgttatgcag gaatacacga atcattccga taccactttt
    12601 gcactgcgta atatctcctt tcgtgtgccc gggcgcacgc ttttgcatcc gctgtcgtta
    12661 acctttcctg ccgggaaagt gaccggtctg attggtcaca acggttctgg taaatccact
    12721 ctgctcaaaa tgcttggccg tcatcagccg ccgtcggaag gggagattct tcttgatgcc
    12781 cagccgctgg aaagctggag cagcaaagcg ttcgcccgca aagtggctta tttgccgcag
    12841 cagcttcctc cggcagaagg gatgaccgtg cgtgaactgg tggcgattgg tcgttacccg
    12901 tggcatggcg cactagggcg ctttggtgcg gcagatcgcg aaaaagttga ggaagctatc
    12961 tcgctggttg gcttaaaacc gctggcgcat cggctggtag acagcctctc tggcggcgaa
    13021 cgtcagcggg cgtggatcgc catgctggtg gcgcaggata gccgttgtct gttgctcgac
    13081 gaaccgactt cggcgttgga tatcgcccac caggttgatg tgctggcgct ggtgcaccgt
    13141 ttaagtcagg agcgtggcct gacggtcatt gccgtgctgc acgatatcaa tatggctgcg
    13201 cgttactgtg attatctggt cgccctgcgc ggcggtgaaa tgattgctca gggaacgcct
    13261 gcggaaatta tgcgcggcga aaccctcgaa atgatttatg gcatcccgat gggtattctg
    13321 ccgcatccgg cgggtgctgc acctgtgagt tttgtttatt gatgagcggc ttacctctta
    13381 tttcgcgccg tcgactgtta acggcgatgg cgctttctcc gttgttatgg cagatgaata
    13441 ccgcccacgc ggcggctatt gacccaaatc gtattgtggc gctggagtgg ctgccggtgg
    13501 aattactact ggcgctcggc atcgtgcctt atggcgtggc agacaccatc aactatcgac
    13561 tgtgggtcag cgaaccacca ctacctgaat cggtgattga cgtcggtttg cgtacagaac
    13621 ctaacctcga actgctgacc gaaatgaaac cgtcgtttat ggtctggtcg gcgggatatg
    13681 gtccttcgcc agaaatgctg gctcgtattg cgccggggcg tggatttaac ttcagcgacg
    13741 gcaaacagcc gctggcgatg gcgcgtaaat cgctgacgga aatggcggat ttacttaacc
    13801 tgcaaagcgc agcagaaacg catttagcgc aatatgaaga ctttatccgc agcatgaacc
    13861 cccgctttgt gaagcgtggt gcgcgtccgt tattgctgac gacgcttatc gatccgcgcc
    13921 atatgctggt cttcggtcca aacagcttgt tccaggaaat tcttgatgag tacggcatcc
    13981 caaatgcctg gcaaggggaa accaacttct ggggcagtac cgccgtcagc atcgatcgtc
    14041 tggcagcgta taaagacgtt gatgtgctct gttttgatca cgacaacagc aaagacatgg
    14101 atgcgctaat ggcaacgccg ctgtggcagg ccatgccgtt tgtccgcgcc ggacgctttc
    14161 agcgcgtacc cgcagtctgg ttttatggtg caactctctc ggcaatgcac tttgtgcgcg
    14221 ttctggataa cgccattgga ggtaaagcgt gagtaaacgt attgcgcttt tcccggcatt
    14281 attgctggcg ttgctggtga ttgccgctac ggcgctgacc tggatgaact tctcgcaggc
    14341 gctgccgcgt agccagtggg cgcaggctgc ctggtcgccg gatattgacg tcatcgagca
    14401 gatgattttt cactacagct tgctgccgcg tctggcgatt tcgctgctgg tgggcgcagg
    14461 cctggggctg gtgggcgtgc tgtttcagca agtgctgcgt aatccgctgg cggaaccgac
    14521 gacacttggc gttgctacag gcgcgcaact ggggattacc gtcactacgc tctgggcgat
    14581 cccaggggcg atggcgagtc agtttgctgc gctggcgggc gcttgcgttg ttggcttaat
    14641 cgtctttggc gtcgcgtggg ggaaacggct ttcgccggta acgctgatcc tcgcggggct
    14701 ggtagtgagc ctttattgtg gggcaatcaa tcagttactg gttatcttcc accatgacca
    14761 actgcaaagc atgttcctgt ggagcaccgg aacgctgacg caaaccgact ggggcggtgt
    14821 tgagcgttta tggccgcagt tgctgggcgg tgtgatgctg acgttgctgc tacttcgtcc
    14881 gttaacactg atggggcttg atgatggcgt ggcgcgcaat ctcgggctag ccttgtcgct
    14941 ggcgcgtctg gcggcgctgt cgctggctat tgtcatcagt gcgctgctgg tgaacgcggt
    15001 ggggattatc ggctttatcg ggttgttcgc gccgttgctg gcaaaaatgc tgggggcgcg
    15061 gcgtctgctg ccgcgactga tgctggcgtc gttgattggt gcgctgatcc tctggctttc
    15121 cgatcaaatc atcctctggc tgactcgcgt gtggatggaa gtgtccaccg gttcggtcac
    15181 tgcgttgatc ggtgcgccgc tgctactgtg gctgttgccg cgtttacgca gcattagcgc
    15241 gccggatatg aaggtcaacg atcgtgtcgc ggctgaacgc caacatgtgc tggcgtttgc
    15301 cctcgcgggc ggcgtgctgc tgttgatggc tgtggtggtg gcgctgtcgt ttggtcgtga
    15361 tgcgcacggc tggacgtggg cgagcggggc gttgctcgag gatttgatgc cctggcgctg
    15421 gccgcgaatt atggcggcgc tgtttgcggg cgtcatgctg gcggtggcgg gctgtattat
    15481 tcagcggctg accggaaacc cgatggcaag cccggaagtg ctggggatta gctctggcgc
    15541 ggcgtttggc gtggtactga tgctgtttct ggtaccgggt aatgcttttg gctggctgtt
    15601 acccgcggga agtatcggtg cggcggtgac gctgttgatt attatgatcg ccgccgggcg
    15661 cggtggattt tccccacacc gcatgttact ggcggggatg gcgttaagta ccgcattcac
    15721 catgctttta atgatgttgc aggcgagtgg tgacccgcga atggcgcaag tgctgacctg
    15781 gatctctggt tcgacctaca acgccaccga tgcgcaggtc tggcgcaccg gaattgtgat
    15841 ggtgattttg ctggcgatta ccccgctgtg ccgccgctgg ctgaccattt taccgctggg
    15901 tggtgatacc gcgcgggccg tgggggtggc gttaacgccg acgcgaattg cgctgctgct
    15961 gttagcggct tgcctgacgg cgaccgcgac gatgaccatt ggaccgttga gttttgttgg
    16021 tttaatggca ccgcatatcg cgcggatgat gggctttcga cggacgatgc cgcacatcgt
    16081 gatttcggcg ctggtgggtg gtttactgct ggtgttcgct gactggtgtg ggcggatggt
    16141 gctgtttccg ttccagatcc cggcggggct gctgtcgacc tttatcggcg cgccatattt
    16201 tatctatttg ttgagaaagc agagccgtta ataaaatgcc tcgcccggtg ggcgaggcaa
    16261 aatcatcaca acttcgcaaa cacccgacgt gcagcatcga tggtgttatt gatatcttcc
    16321 atgctgtgcg ccacagacat aaagcccgct tcaaacgctg acggtgccag gtaaacacct
    16381 tcatccagca tcatatggaa gaaacgctta aaacgctcga cgtcacaggc catcacatcc
    16441 tgatagcacg tcacggactc ggcgtcggta aagaaaatac cgaacatgcc gccaacgtgg
    16501 tttaccacca gcggaattcc ggcttcttct gccgcttcca gcagaccttc tgccagacgt
    16561 gttgtcagct catccagcgt ttcgtgaacg cccggctgcg cgacttcatt cagacaggcg
    16621 aaaccggctg ccatcgcaat cgggttaccg gaaagcgtac ccgcctgata gaccggaccc
    16681 gtcggggcca gcgcatccat tacatcacga cgaccaccga atgcgcctac cggcattcca
    16741 ccgccgatga ttttgcccag gcaagtgaga tccggttcca caccgtaata atcctgtgcg
    16801 ccagctagcg ctacgcggaa accggtcatt acttcatcga tgatcagcaa cgcgccaaat
    16861 tcgtcgcaca gcgcgcgcag acctggcagg aagtctggca gcggtggaac acagttcata
    16921 ttgcctgcca ccggctcgac gataatacag gcaatctctt gcgggtattg ctcaaacgcg
    16981 gcgcgtacag aagccagatc gttataagta caggttaagg tatgtttggc gaaatctgcc
    17041 ggaacgcccg gcgagtttgg ctggcctaac gtgagtgcgc cagagccggc tttcaccagc
    17101 aggcagtcag cgtgaccgtg gtaacaacct tcaaatttaa taattttgtc gcgaccggta
    17161 aaaccacggg ccaggcggat ggcgctcatt gtcgcttcag tgccggagtt caccatgcgc
    17221 accatatcca tggtcgggac cagttcggtc accagttgcg ccattttcac ttccatttcg
    17281 gttggtgcac caaagcttaa accacgctcg gcggcttcaa tcacggcatt gcggatcgcc
    17341 ggatggttat ggcccagcac catcggcccc caggaaccga cataatcgat ataggctttg
    17401 ccatcaacat cgtacagata agcgccgtcc gctttttcga taaacagtgg agtgccgccc
    17461 acgccagtaa aggcgcgaac aggggagttc acaccgccag ggatcagctc gcgcgctgcg
    17521 ctgtaaagat tttcagactt actcatggag ggttcctgat tcgtagaaaa agtgaatggc
    17581 tgctattcta tgttattcat aacaagttaa atactcgtca aacatcaggc tgcttgtact
    17641 ggtgcaatcc tgatttctta gagtataaaa gttttgtgca tttgaaacat tacgctttgc
    17701 aaaggatttt catggaacgt gcgagtaaaa tgccgtcatc ttatttgtat gaccaataag
    17761 tgatcattgg atgaaaactg atactccctc tttagaaaca ccgcaggccg cgcgcctgcg
    17821 acgcagacaa ctgattcgcc aacttcttga gcgcgataaa accccgttag ccattttgtt
    17881 tatggcggca gtcgtcggca cgcttgtcgg gctggcagcg gttgcttttg acaaaggtgt
    17941 cgcctggttg cagaaccaac gtatgggggc gctggtacat actgctgata attatccgct
    18001 actgttaacc gttgcttttc tctgttcggc ggtgctggcg atgttcggct actttttggt
    18061 gcgcaaatac gcgcccgaag caggggggtc ggggatcccg gaaattgaag gggcgctgga
    18121 agatcaacgt cctgttcgct ggtggcgtgt attgccggtg aaattctttg gcgggctggg
    18181 gacactcggc ggaggcatgg tgttggggcg cgaagggcca accgtgcaga tcggcggtaa
    18241 tatcgggcgg atggtactcg atgttttccg cctgaaaggt gatgaagctc gccatacgct
    18301 gttggcaacc ggtgcggcgg cgggtctggc ggcggccttt aacgcgccgc tggcgggtat
    18361 tttgtttatt atcgaagaga tgcgtccgca gtttcgctat acgttaattt cgattaaagc
    18421 ggtatttatt ggcgtcatta tgtcgaccat tatgtaccgg atttttaatc atgaagttgc
    18481 gttgattgac gtcggtaaac tttctgacgc gccgcttaat acgctgtggc tttatctgat
    18541 cctcggtatt atttttggca ttttcggccc tatttttaat aaatgggtgc tggggatgca
    18601 ggatttgctg caccgtgtgc acggtggcaa tattaccaaa tgggtactga tgggtggtgc
    18661 gattggcggt ctgtgtggat tgctggggtt tgtggcacca gcaacgtcgg gcggcggttt
    18721 taacctgatt cctatcgcta ccgcggggaa tttcagcatg ggaatgctgg tgtttatctt
    18781 cgtcgcgcgg gtcattacca ccttactctg cttctcttcc ggcgcgccgg gcggtatttt
    18841 tgccccgatg ctggcgctgg gtactgtgct gggaaccgct ttcggaatgg ttgccgttga
    18901 gctgtttccg caatatcacc ttgaggcggg gacgtttgct attgccggaa tgggggcgtt
    18961 actggcggca tctattcgcg cgccgttaac ggggatcatt ctggttctgg agatgaccga
    19021 taactaccag ctcattttgc caatgattat taccggtctt ggcgcaacac tattagcgca
    19081 atttaccggc gggaaaccgc tatactcggc gattcttgcg cgcacgctgg caaaacagga
    19141 agctgagcaa ctggcgcgaa gcaaggccgc atcagccagc gagaatactt gaacgaaata
    19201 ccagggtatt agataatggc gattattatt gggttagaat ttgcccaatt gccgatgtcg
    19261 tttggagcaa aatatgagtg atgacgtagc actgccgctg gagtttaccg acgcagcagc
    19321 caacaaagtt aaaagcctga tcgctgacga agataacccg aatctgaaat tacgcgtgta
    19381 tatcaccggt ggcggttgca gcggcttcca gtatggtttc acctttgatg atcaggtgaa
    19441 cgaaggcgat atgaccatcg aaaaacaggg cgttggcctg gtggttgatc cgatgagcct
    19501 gcaatatctg gtcggcggtt ccgttgacta taccgaagga ctggaaggtt ctcgtttcat
    19561 cgtgaccaac ccgaacgcga aaagcacctg cggttgcggt tcttccttta gtatctaatc
    19621 tgttgttcat gtgccggata aactatccgg cacatcttct ctcttcaacg cccattctca
    19681 tccagcgcaa acgtcggtag cttaagatgc caacgaatag ccgccagccg aatcaatagc
    19741 gtcacgacca tgcccatcat actggctgtt tccagtggta cggaaaatgt gtaataagcc
    19801 gtagcgtgga caataccgcc gataatacag gcagttgcgt agatttctgt acgtaaaatc
    19861 atggggattt cgcgggccag aacatcacga atgatcccgc cgccaacgcc agtaatgacg
    19921 cccatacaaa ccgcgattaa cggaccggct tccgcattaa aggctttatt cacgccaatg
    19981 ccgacaaaca ccgccagacc aacggcgtcc agcaccggca acatccattt tggtaagtgt
    20041 cttggctggc gcaccagcac gatggtcagc atgctggtta ccattgcaac gaccagatcg
    20101 gtgggatctt tcacccaaaa taccgggccg tgatccagcg ccatgtcgcg aattgtcccg
    20161 ccgcctacag cggtaaccac gcccagtacc agaacaccaa aagggtccat acgcaatttt
    20221 ccggctaaca aaacgccgga gatggcaaat accgctgtgc cgactatatc cagccaatag
    20281 acgagcattg ttcaatcccc actgagcacc taaaaaggtc aggcgctaat ctacctgtga
    20341 aagcgcatta cagagctgtt gtgcagcgag gataatacgt gggcttgcac gttcaaacca
    20401 gtcactcgtg agaggaataa cgggaatttt gagctgttca ccccagtatt gtttgatttt
    20461 aggaatttgg tccggtccgc ctgtaatgac aatcgcctgt ggcgagcgtg ctaacacctg
    20521 ttcgcggcta acttgcggcc agggaacccg gctgtcttta aagatgtttt ctccgccaca
    20581 aacttcgagt acctggtttt gaatcgactc ttttccactg gtaaatggcg gattaatgcc
    20641 gaattgcaga aaaacacgtt ttttaggttt atcagcatat tgcgctttca attgcgcgta
    20701 ttgatccagc agggattgcg cggcttgttc ggccttatcc ggttgcggac tccagggagc
    20761 cagttgacgt aacgcattgg caatttgttc aatgtttgtc gcatcgaccc acatcacttt
    20821 tattcccagc gaagccagct ggtcaacctg ccgctcggca ttacctccac gccaggcaat
    20881 caccagatcg ggtttcagcg cgacaatgcg ttccagattc atcccctgcc aggtggaaac
    20941 ctgctcaatc ttttgcgctt gtggaggata gtcggaatag ctgctgaccc caaccggcgt
    21001 gatcccggcg gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga cgcgcggcgc
    21061 ggcgttgagc cacagtggcg caagaaaaga cagggcgacc agcgccctga acagtgactt
    21121 agccatgtgc cagtttctgc actagtgact caaccatcag gctggactgt ttagcggcaa
    21181 cagccaggaa ctcatcgaag ctaagatgag actgttgatc ggccacgtcg gagatggcgc
    21241 gtacgacaac aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc gtcgcttcca
    21301 tctctacagc aatggcctgt gggaagttgt ggcggatttt cgccagacca acagaaccgt
    21361 tgatgaaagc gtcgccgcta acaatcaggc cacgtacagc gttaagattc agttcggcaa
    21421 tgcaggcctc agcggcagcg atcagtttat cgtcagcttt aaagcctgcc ggacagcctg
    21481 gtaactgacc gtattcataa ccaaatgccg tgacatccgc gtcgtgataa cgcgcttcgt
    21541 ccgagacaac gatatcgccc actttcagcg ttggtgccag gccaccggca gaaccggtgt
    21601 taataatcac atctggcttg cagtgttcca acagcagagt ggcacccagc gccgcagcga
    21661 ctttaccgat gcccgatttc agaagcgcaa cctcggttcc attcagttgg ccggtataga
    21721 tttcgcaacc gcccagactg atagtttgac ggttttcgat tttgtcacgc agcagcgtaa
    21781 cttcttcttc cattgcacca atgatgccga ttttcataga tttactcgcg ataagcccga
    21841 tttgaaggca tagtttacca tgcgcttacg gggaagcgta tttctcacgc gggagaggac
    21901 atggcacaga ttgatttccg aaaaaaaata aactggcatc gtcgttaccg ttcaccgcag
    21961 ggcgttaaaa ccgaacatga gatcctgcgg atcttcgaga gcgatcgcgg gcgtatcatc
    22021 aactctccgg caattcgtcg tctgcaacaa aagacccagg tttttccact ggagcgcaat
    22081 gccgccgtgc gcacgcgtct tacccactcg atggaagtcc agcaggtggg gcgctacatc
    22141 gccaaagaaa ttttaagccg tctgaaagag cttaaattgc tggaagcata cggcctggat
    22201 gaactgaccg gaccttttga aagcattgtt gagatgtcat gcctgatgca cgatatcggc
    22261 aatccgccgt ttggtcattt tggcgaagcg gcgataaatg actggtttcg ccagcgtttg
    22321 cacccggaag atgccgaaag ccagcctctg actgacgatc gctgcagcgt ggcggtacta
    22381 cgtttacggg acggggaaga accgcttaac gagctgcggc gcaagattcg tcaggactta
    22441 tgtcattttg aggggaatgc acaaggcatt cgtctggtgc atacattgat gcggatgaat
    22501 ctcacctggg cgcaggttgg cggtatttta aaatataccc gtccagcgtg gtggcgtggc
    22561 gaaacgcctg agacacatca ctatttaatg aaaaagccgg gttattatct ttctgaagaa
    22621 gcctatattg cccggttgcg taaagaactt aatttggcgc tttacagtcg ttttccatta
    22681 acgtggatta tggaagctgc cgacgacatc tcctattgtg tggcagatct tgaagatgcg
    22741 gtagagaaaa gaatatttac cgttgagcag ctttatcatc atttgcacga ggcgtggggc
    22801 cagcatgaga aaggttcgct cttttcgctg gtggttgaaa atgcctggga aaaatcacgc
    22861 tcaaatagtt taagccgcag tacggaagat cagtttttta tgtatttacg ggtaaacacc
    22921 ctaaataaac tggtacccta cgcggcacaa cgatttattg ataatctgcc tgcgattttc
    22981 gccggaacgt ttaatcatgc attattggaa gatgccagcg aatgcagcga tcttcttaag
    23041 ctatataaaa atgtcgctgt aaaacatgtg tttagccatc cagatgtcga gcagcttgaa
    23101 ttgcagggct atcgggtcat tagcggatta ttagagattt atcgcccttt attaagcctg
    23161 tcattatcag actttactga attggtagaa aaagaacggg tgaaacgttc ccctattgaa
    23221 tctcgcttat tccacaaact ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt
    23281 aaattaccgt cagattctcc tgagtttccg ctatgggaat attattaccg ttgccgcctg
    23341 ctgcaggatt atatcagcgg tatgaccgac ctctatgcct gggatgaata ccgacgtctg
    23401 atggccgtag aacaataacc aggcttttgt aaagacgaac aataaatttt taccttttgc
    23461 agaaacttta gttcggaact tcgcgctata aaacgaatct gaagaacaca gcaattttgc
    23521 gttatctgtt aatcgagact gaaatacatg aaaaaaacca cattagcact gagtgcactg
    23581 gctctgagtt taggtttggc gttatctccg ctctctgcaa cggcggctga gacttcttca
    23641 gcaacgacag cccagcagat gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca
    23701 gtggtcagca ttaacgtaga aggtagcaca accgttaata cgccgcgtat gccgcgtaat
    23761 ttccagcagt tcttcggtga tgattctccg ttctgccagg aaggttctcc gttccagagc
    23821 tctccgttct gccagggtgg ccagggcggt aatggtggcg gccagcaaca gaaattcatg
    23881 gcgctgggtt ccggcgtcat cattgatgcc gataaaggct atgtcgtcac caacaaccac
    23941 gttgttgata acgcgacggt cattaaagtt caactgagcg atggccgtaa gttcgacgcg
    24001 aagatggttg gcaaagatcc gcgctctgat atcgcgctga tccaaatcca gaatccgaaa
    24061 aacctgaccg caattaagat ggcggattct gatgcactgc gcgtgggtga ttacaccgta
    24121 gcgattggta acccgtttgg tctgggcgag acggtaactt ccgggattgt ctctgcgctg
    24181 gggcgtagcg gcctgaatgc cgaaaactac gaaaacttca tccagaccga tgcagcgatc
    24241 aaccgtggta actccggtgg tgcgctggtt aacctgaacg gcgaactgat cggtatcaac
    24301 accgcgatcc tagcaccgga cggcggtaac atcggtatcg gttttgctat cccgagtaac
    24361 atggtgaaaa acctgacctc gcagatggtg gaatacggcc aggtgaaacg cggtgagctg
    24421 ggtattatgg ggactgagct gaactccgaa ctggcgaaag cgatgaaagt tgacgcccag
    24481 cgcggtgctt tcgtaagcca ggttctgcct aattcctccg ctgcaaaagc gggcattaaa
    24541 gcgggtgatg tgatcacctc actgaacggt aagccgatca gcagctttgc cgcactgcgt
    24601 gctcaagtgg gtactatgcc ggtgggcagc aaactgaccc tgggcttgct gcgcgacggt
    24661 aagcaggtca acgtgaatct ggaactgcag cagagcagcc agaatcaggt tgattccagc
    24721 tccatcttca acggcattga aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg
    24781 gtagtgaaca acgtgaaaac gggcactccg gctgcgcaga tcggcctgaa gaaaggtgat
    24841 gtgattattg gcgcgaacca gcaggctgtg aaaaacatcg ctgaactgcg taaagttctc
    24901 gacagcaaac cgtctgtgct ggcactgaac attcagcgcg gcgacagcac catctacctg
    24961 ttaatgcagt aatctccctc aaccccttcc tgaaaacggg aagggggtct ccttacaatc
    25021 tgtgaacttc accacaactc catacatctt catcatcctt taggcatttg cacaatgccg
    25081 tacgttacgt acttccttat gctaagccgt gcataacgga ggacttatgg ctggctggca
    25141 tcttgatacc aaaatggcgc aggatatcgt ggcacgtacc atgcgcatca tcgataccaa
    25201 tatcaacgta atggatgccc gtgggcgaat tatcggcagc ggcgatcgtg agcgtattgg
    25261 tgaattgcac gaaggtgcat tgctggtact ttcacaggga cgagtcgtcg atatcgatga
    25321 cgcggtggca cgtcatctgc acggtgtgcg gcaagggatt aatctaccgt tacggctgga
    25381 aggtgaaatt gtcggcgtaa ttggcctgac aggtgaacca gagaatctgc gtaaatatgg
    25441 cgaactggtc tgcatgacgg ctgaaatgat gctggaacag tcgcggttga tgcacttgtt
    25501 ggcgcaggat agccgtttgc gggaggaact ggtgatgaac ctgattcagg cagaggagaa
    25561 tactcccgca cttactgaat gggcgcaacg gctggggatc gatctcaatc aaccgcgagt
    25621 ggtggctatt gttgaggtcg acagcggtca gcttggtgtg gacagcgcaa tggcggagtt
    25681 acaacaactg caaaacgcgc tgactacgcc cgagcgtaat aatctggtgg cgattgtctc
    25741 gctaaccgaa atggtggtgt tgaaaccggc gttgaactct tttgggcgct gggatgtaga
    25801 agatcatcgt aagcgagttg aacaactgat tacccgcatg aaagagtacg gccagctgcg
    25861 ttttcgcgtt tcactgggca actattttac cggtcctggc agtattgccc gatcctatcg
    25921 tacggcgaaa acgacgatgg tggtgggtaa acagcggatg ccagaaagtc gctgctattt
    25981 ttatcaggat ctgatgttac ctgtgttact cgacagtttg cgtggcgact ggcaggccaa
    26041 cgaactggcg cgaccgctgg cgcggctgaa agcgatggac aataacggct tgctgcgacg
    26101 aacgctggcg gcgtggtttc gtcacaatgt gcaaccgctg gcaacgtcaa aggcgttgtt
    26161 tattcatcgt aataccctgg agtatcggct taatcgtata tcggaactga ccgggcttga
    26221 tttgggcaat tttgatgaca ggttgctgct gtatgtggcg ttacaactgg atgaagagcg
    26281 gtaggttatg cgttaaggtt gaggcggcgc tggctcattc ccctcagccc gaaggggaga
    26341 ggggaatgcc cgagttaacc gtcaattcgg gattatttac gcgttagttt ttccagatcg
    26401 gcttcaatct cgctgatctt attggttacg actgactcca ggtgacgtaa gtcgtcgagg
    26461 atcttacgct taagatcaac ttcgctgcgg tcacgctggc agatttgatc aagctcatca
    26521 atgatatacc gtagattcgg gctgatttcc tggacttctt tataaccctg acccacacca
    26581 tcagcgacga ccgttttacg ctgacgcgga tatttaaact taacgctctt ggcgaaaaac
    26641 tcgcctttgt ctttctggaa atagattttc agaatatcgt tgttggcttc ctgccggagg
    26701 ctgtaacgat caatttcttc aggattggta atacccagac ttttcagatt gtcgtacata
    26761 gcgttacctc aaaatgagtc agtaaaatgt gcttatttta gcatttggcc tggcccgccc
    26821 ggctcgactg tgatcgcaga aagccttttt cttccagact tctatcatgg cgtaaaaatc
    26881 aaaaaattac ctgctttatt gtggcgatat tccatgggat ggattagcac atttatcttc
    26941 gttttgcgaa ttacatgaca gtccgaaaca agatgaaaaa gtatttaaaa aaggggggag
    27001 gaagaatgag taggacgaat tattctgaac cagaaagcaa tcgggcagcg tcgtgctgcc
    27061 cgtgtgcata cttttagtcg atggtacgca gcagttcgtt aatgccgact ttgccgcgag
    27121 ttttcgcgtc aactttctta acgataaccg cacagtagag gctgtatttg ccatctttcg
    27181 acggcagatt acctgaaaca accacagacc ccgccggaac gcgaccgtag tggatgtcgc
    27241 cggtttcacg gtcgtaaata cgggtgctct gaccaatgta tacgcccatg gaaatgacgg
    27301 aaccttcttc gacaatcacc ccttcaacta cttcagagcg cgcgccgatg aagcaattat
    27361 cttcaatgat ggttgggtta gcctgcagcg gttccagcac accgccgatg cccacgccac
    27421 cggaaaggtg gacgttttta ccaatctgcg cacaagaacc gacggtcgcc caggtatcga
    27481 ccatggtgcc ttcatcaaca tatgcgccga tgttgacgta agacggcatc agcacggtgt
    27541 tacgggcaat aaacgcaccc tgacgtaccg ccgcaggtgg tacaacgcgg aagccttctt
    27601 tctggaaacg tgcttcgtcg tagtcggcga atttcatcgg cactttgtcg aagtagcggc
    27661 tttctgcccc ttcgatcacc tgattatcat taatacggaa agagagcagc accgcttttt
    27721 tcaaccactg atgcgtcacc cactgaccgt caattttttc cgctacacgc agtgcgccgg
    27781 aatccagcag ggcgatcacc tgatttaccg cttcgcgggt aacggtgtct gcattggctg
    27841 gcgtgatctc ggcacggcgt tcaaaagcgg tttcaataat gttctgtaac tgctgcattg
    27901 ttaaactctt ttcatatcag taaacacatc accctttatc gtttggattg agggcctctg
    27961 tcaaccgctg atgcacttcc tgctgcaact cgttattaag tgcacgccgg tcagcggtgg
    28021 caattatgaa taaatcttct actcgctcgc caatggttgt aattcgggca ccatgaagcg
    28081 aaattcccag atcggcaaaa attttcccga ctctcgccag cagtcctggc tggtcgagcg
    28141 cgataagctc aaggaacgat ttgcggtcgg tatgggttgg caaaaacgtc acttcggttt
    28201 caacagtaaa gtgacgtagt ttagccggtt gacgacgcgg ctgtggtggc tgccagctac
    28261 tttgtgtcag tacttgctcc agaccaaacc gaataacctc atgacgatct gcggatagcg
    28321 ggctaccatc gggttccagc acgataaagg tatccatcgc cataccgtcg cgagtggtga
    28381 aaatttgtgc gtcgtgaacg cttaagttgc gtctatctaa ctcggcacaa acagcagcaa
    28441 acaagtaagg gcggtccggg ctccagataa aaatctcggt gcctccacgc gtagcctgcg
    28501 ggctaagcaa taccagcggt ttgcttaaat catgctgtaa taaatggcgc gcatgccagg
    28561 ccagttgatt tgggctatga cggacaaaat agttcgcgcg acagcgtgac caaatttggt
    28621 gcagcacctc ttcgtcgatg ttatccatgc gcagtagtgc cagcgcctgg agttggtgat
    28681 ggcgaacccg ttcgcgcata tccggcgtgt tttgcattcc gcgtcgtagc tgcttttcgg
    28741 tggcgaaata gagttcgcgc aacagacttt gcttccagct attccacagc gtttcgttgg
    28801 tggcgcaaat gtcagccact gtcaggcata ccagatagcg cagacgattt tccgtttgaa
    28861 cttcttcggc aaactgcttg atgacttccg ggtcctgaat atcgcgacgc tgagctgtca
    28921 ccgacatcag caagtgctgg cgaaccagcc aggcgaccag ctgtgtttcg cgtgagttca
    28981 gcccgtggag ttcggcaaaa tgcactacat cctgagcacc gagaatagag tggtcgccgc
    29041 cgcgtccttt ggcgatatcg tgaaacagcg cggcgatgaa aatcagctca gttgacggta
    29101 ggcgcggcca gacgttcaca cacaacggat ggcgctggcg cgtttcttca ctggcaaaac
    29161 tctccagttt cagcatcacg cggatagtat gttcatccac cgtgtaggcg tggaacagat
    29221 caaactgcat ctgcccgacg atatgtgacc attgcggcat atacgcgccg agtacgctat
    29281 ggcgatgcat tggcaatagc ccgcgccgca ccgctccggg gtgacgtaga atgctcaaaa
    29341 acagttttcg tgcttgcgga atattacaca gcggttgttg cagatggcga cgggcatggc
    29401 gtaactggcg cagtgtggtg gagtaaatgc cggtgatcgc actgttgcgc accatggtgt
    29461 agaacatacg caagatggct tccggctggc gcataaatag tgtttcatca cgcaggtcga
    29521 ttagcgtacc gcgtagctga aactcatcgt cgattggacg tggtttttcg tcggcgggaa
    29581 gggcgaggat ggcttcatcg aacagttgca gcagcatctg gttgagttca ctgacgcggc
    29641 gtgtcacgcg gaagtaatcc ttcatcatcc gctcgaccgg ttcgttacct tcgccactgt
    29701 aattcagacg ctgggcgacg ctaagctggc gatcgaataa caggcgatta tcgtaacggc
    29761 tgacgaccag atgcagggca aagcgaatac gccacaatat atgcagacat tcgtttaatt
    29821 ccgcccgctc cgctgaggtt aagaagccaa acccaaccat ttcatccagc gatgttgcgc
    29881 caaaatgacg gcgggccacc cattgcagag tgtggatatc gcgcaagccg ccagggctac
    29941 ttttgatgtc tggttcaaga ttgtagctgg tgccgtggta acgctgatgg cgctggttct
    30001 gttcttcaac tttcgccgcg tagaacttgt cggaaggcca gaatccttcg ctgaaaatat
    30061 gtttttgcag ttcaagaaat agcgcaacat cgccaattaa taagcgggat tcgatcaaat
    30121 tggtggcgac ggttaaatcc gataaccctt ccagcatgca ctcttcaagc gtgcgcacac
    30181 tatgaccgac ttccagcttt acatcccaga gcagcgttaa cagctcgccc actttttgcg
    30241 cctgatcgtc cgggagcttt ttacggctta aaatcagtaa atcgatgtca gaaagtggat
    30301 gcagctcgcc acgaccgtag ccaccgacgg cgaccaatgc caggtcggca atctggctga
    30361 atcccgcttc aatccataat cgttgcagga gctggtcgat aaactcggtg cgcgcctcaa
    30421 tcaactgttc tgcagagatc ccattgtcaa aggcatcacc cagccaacgc tggaaagtat
    30481 cgatatgggc ttttatccca ccgacggtta attcatcacg gggccagacg catggatttt
    30541 gcggttgacc gggcagggtg gggagagcgg tgtttgcgta ctgttctgga agggtattca
    30601 ttgtgcgcca cccataagat taaattatcg cattaaaaaa gccggcattc gccggcttca
    30661 ttttattcgt cgtgcgagat tatcgccggg atggtgtcat ccttgcgtag cgtcagaatt
    30721 tcgcagccgt tatcagtcac cacaatagta tgctcatatt gtgcagacaa gctgcgatct
    30781 ttggttttta ccgtccagcc atctttcatg gtgcggatct cttttttacc cgcgttgacc
    30841 attggctcga tggtgaacgt catcccaggt ttcagtacga cgttggtttc acgggagtca
    30901 tagtgcagca cctgcggttc ttcatggaag ccgcgaccaa taccgtgtcc gcaatattca
    30961 cgaacgacgg agaagccttc tgcttcgaca aatttctgaa tcgccgcacc gatttcgcgc
    31021 agattaatgc ctggttttac catgcgtagc gccaggtaca ggctttcttg cgtgatacgg
    31081 cacagacgtt cgcccatgat ggtcggctta ccgacgataa acattttcga ggtatcgccg
    31141 tggaaaccat ctttgattac ggtgacatca atgttaacga tatcgccatc tttcagcagc
    31201 ttagcatcgt ccggaatacc gtggcacacc acttcattaa tagaaatgca aacggatttc
    31261 ggataaccgt gatagccgag gcaggctgaa accgcgtgtt gttcattaac aatgtaatca
    31321 ttacagatgc gatccagctc gccggtgctg acgcccggtt taacatatgg ttcgatcatc
    31381 tccagcactt cggcagccag tcggccagcg acgcgcattt tttcgatatc ttctggggtc
    31441 ttgattgaga tagccattaa ttctgtccat cagcgtcggt gataccgaca atatatatgt
    31501 aagtgccgtc aatggtatca cacccgggca aattgagaat cattctgaat ttcgccaaac
    31561 gtgccactga aggttttcta taatagaaaa ttcgacgtct gatgctgtac acagcgccaa
    31621 caattattgg tgtccacggc gtatttgtgg tataaagcgc gccggacttc cgatccattt
    31681 cgtatacaca gactggacgg aagcgacaat ctcactttgt gtaacaacac acacgtatcg
    31741 gcacatattc cggggtgccc tttggggtcg gtaatatggg atacgtggag gcataacccc
    31801 aacttttata tagaggtttt aatcatggca actgtttcca tgcgcgacat gctcaaggct
    31861 ggtgttcact tcggtcacca gacccgttac tggaacccga aaatgaagcc gttcatcttc
    31921 ggtgcgcgta acaaagttca catcatcaac cttgagaaaa ctgtaccgat gttcaacgaa
    31981 gctctggctg aactgaacaa gattgcttct cgcaaaggta aaatcctttt cgttggtact
    32041 aaacgcgctg caagcgaagc ggtgaaagac gctgctctga gctgcgacca gttcttcgtg
    32101 aaccatcgct ggctgggcgg tatgctgact aactggaaaa ccgttcgtca gtccatcaaa
    32161 cgtctgaaag acttggaaac tcagtctcag gacggtactt tcgacaagct gaccaagaaa
    32221 gaagcgctga tgcgcactcg tgagctggag aaactggaaa acagcctggg cggtatcaaa
    32281 gacatgggcg gtctgccgga cgctctgttt gtaatcgatg ctgaccacga acacattgct
    32341 atcaaagaag caaacaacct gggtattccg gtatttgcta tcgttgatac caactctgat
    32401 ccggacggtg ttgacttcgt tatcccgggt aacgacgacg caatccgtgc tgtgaccctg
    32461 tacctgggcg ctgttgctgc aaccgtacgt gaaggccgtt ctcaggatct ggcttcccag
    32521 gcggaagaaa gcttcgtaga agctgagtaa taaggcttga taactccccc aaaatagttc
    32581 gagttgcaga aaggcggcaa gctcgagaat tcccgggagc ttacatcagt aagtgaccgg
    32641 gatgagcgag cgaagatgac gcatctgcgg cgcgaaatat gaagggggag agcccttata
    32701 gaccaggtag tacacgtttg gttagggggc ctgcatatgg cccccttttt cacttttata
    32761 tctgtgcggt ttaatgccgg gcagatcaca tctccgagga ttttagaatg gctgaaatta
    32821 ccgcatccct ggtaaaagag ctgcgtgagc gtactggcgc aggcatgatg gattgcaaaa
    32881 aagcactgac tgaagctaac ggcgacatcg agctggcaat cgaaaacatg cgtaagtccg
    32941 gtgctattaa agcagcgaaa aaagcaggca acgttgctgc tgacggcgtg atcaaaacca
    33001 aaatcgacgg caactacggc atcattctgg aagttaactg ccagactgac ttcgttgcaa
    33061 aagacgctgg tttccaggcg ttcgcagaca aagttctgga cgcagctgtt gctggcaaaa
    33121 tcactgacgt tgaagttctg aaagcacagt tcgaagaaga acgtgttgcg ctggtagcga
    33181 aaattggtga aaacatcaac attcgccgcg ttgctgcgct ggaaggcgac gttctgggtt
    33241 cttatcagca tggtgcgcgt atcggcgttc tggttgctgc taaaggcgct gacgaagagc
    33301 tggttaaaca catcgctatg cacgttgctg caagcaagcc agaattcatc aaaccggaag
    33361 acgtatccgc tgaagtggta gaaaaagaat accaggtaca gctggatatc gcgatgcagt
    33421 ctggtaagcc gaaagaaatc gcagagaaaa tggttgaagg ccgcatgaag aaattcaccg
    33481 gcgaagtttc tctgaccggt cagccgttcg ttatggaacc aagcaaaact gttggtcagc
    33541 tgctgaaaga gcataacgct gaagtgactg gcttcatccg cttcgaagtg ggtgaaggca
    33601 tcgagaaagt tgagactgac tttgcagcag aagttgctgc gatgtccaag cagtcttaat
    33661 tatcaaaaag gagccgcctg agggcggctt ctttttgtgc ccatcttgta aattcagcta
    33721 acccttgtgg ggctgcgctg aaaagcgacg tacaatgtcg ctagtattaa ttcatttcaa
    33781 tcgttgacag tctcaggaaa gaaacatggc taccaatgca aaacccgtct ataaacgcat
    33841 tctgcttaag ttgagtggcg aagctctgca gggcactgaa ggcttcggta ttgatgcaag
    33901 catactggat cgtatggctc aggaaatcaa agaactggtt gaactgggta ttcaggttgg
    33961 tgtggtgatt ggtgggggta acctgttccg tggcgctggt ctggcgaaag cgggtatgaa
    34021 ccgcgttgtg ggcgaccaca tggggatgct ggcgaccgta atgaacggcc tggcaatgcg
    34081 tgatgcactg caccgcgcct atgtgaacgc tcgtctgatg tccgctattc cattgaatgg
    34141 cgtgtgcgac agctacagct gggcagaagc tatcagcctg ttgcgcaaca accgtgtggt
    34201 gatcctctcc gccggtacag gtaacccgtt ctttaccacc gactcagcag cttgcctgcg
    34261 tggtatcgaa attgaagccg atgtggtgct gaaagcaacc aaagttgacg gcgtgtttac
    34321 cgctgatccg gcgaaagatc caaccgcaac catgtacgag caactgactt acagcgaagt
    34381 gctggaaaaa gagctgaaag tcatggacct ggcggccttc acgctggctc gtgaccataa
    34441 attaccgatt cgtgttttca acatgaacaa accgggtgcg ctgcgccgtg tggtaatggg
    34501 tgaaaaagaa gggactttaa tcacggaata attcccgtga tggataaata agggtaagat
    34561 tccgcgtaag tatcgcgggg gatcttaccc gtaatatgtt taatcagggc tatacttagc
    34621 acacttccac tgtgtgtgac tgtctggtct gactgagaca agttttcaag gattcgtaac
    34681 gtgattagcg atatcagaaa agatgctgaa gtacgcatgg acaaatgcgt agaagcgttc
    34741 aaaacccaaa tcagcaaaat acgcacgggt cgtgcttctc ccagcctgct ggatggcatt
    34801 gtcgtggaat attacggcac gccgacgccg ctgcgtcagc tggcaagcgt aacggtagaa
    34861 gattcccgta ctctgaaaat caacgtgttt gatcgttcaa tgtctccggc cgttgaaaaa
    34921 gcgattatgg cgtccgatct tggcctgaac ccgaactctg cgggtagcga catccgtgtt
    34981 ccgctgccgc cgctgacgga agaacgtcgt aaagatctga ccaaaatcgt tcgtggtgaa
    35041 gcagaacaag cgcgtgttgc agtacgtaac gtgcgtcgtg acgcgaacga caaagtgaaa
    35101 gcactgttga aagataaaga gatcagcgaa gacgacgatc gccgttctca ggacgatgta
    35161 cagaaactga ctgatgctgc aatcaagaaa attgaagcgg cgctggcaga caaagaagca
    35221 gaactgatgc agttctgatt tcttgaacga caaaaacgcc gctcagtaga tccttgcgga
    35281 tcggctggcg gcgttttgct ttttattctg tctcaactct ggatgtttca tgaagcaact
    35341 caccattctg ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg tgcgccataa
    35401 tcccgaacac ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc gcatggtaga
    35461 acagtgcctg gaattctctc cccgctatgc cgtaatggac gatgaagcga gtgcgaaact
    35521 tcttaaaacg atgctacagc aacagggtag ccgcaccgaa gtcttaagtg ggcaacaagc
    35581 cgcttgcgat atggcagcgc ttgaggatgt tgatcaggtg atggcagcca ttgttggcgc
    35641 tgctgggctg ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt tgctggccaa
    35701 taaagaatca ctggttacct gcggacgtct gtttatggac gccgtaaagc agagcaaagc
    35761 gcaattgtta ccggtcgata gcgaacataa cgccattttt cagagtttac cgcaacctat
    35821 ccagcataat ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca ttttacttac
    35881 cgggtctggt ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa tgacgcccga
    35941 tcaagcctgc cgtcatccga actggtcgat gggacgtaaa atttctgtcg attcggctac
    36001 catgatgaac aaaggtctgg aatacattga agcgcgttgg ctgtttaacg ccagcgccag
    36061 ccagatggaa gtgctgattc acccgcagtc agtgattcac tcaatggtgc gctatcagga
    36121 cggcagtgtt ctggcgcagc tgggggaacc ggatatgcgt acgccaattg cccacaccat
    36181 ggcatggccg aatcgcgtga actctggcgt gaagccgctc gatttttgca aactaagtgc
    36241 gttgacattt gccgcaccgg attatgatcg ttatccatgc ctgaaactgg cgatggaggc
    36301 gttcgaacaa ggccaggcag cgacgacagc attgaatgcc gcaaacgaaa tcaccgttgc
    36361 tgcttttctt gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt tatccgtact
    36421 ggaaaaaatg gatatgcgcg aaccacaatg tgtggacgat gtgttatctg ttgatgcgag
    36481 cgcgcgtgaa gtcgccagaa aagaggtgat gcgtctcgca agctgaggat aatccggcta
    36541 cagagagtcg cgctatttgt tagcgtaggg cttcagtgat atagtctgcg ccatctgatc
    36601 gtaagtagtt ggctttataa ggtcaggtat gccgtggttt tacacggctt ttttttgtat
    36661 aggcttcagt attcctgagt accgtaaacc ctgtcaggga ataaaaaacg cgtgatgttg
    36721 tctgctactc aaccacttag cgaaaaattg ccagcgcatg gctgccgtca tgttgcgatc
    36781 attatggacg gcaatggtcg ctgggcaaaa aagcaaggga agattcgtgc ctttgggcat
    36841 aaagccggag caaaatccgt ccgccgggct gtctcttttg cggccaacaa cggtattgag
    36901 gcgttaacgc tgtatgcctt tagtagtgaa aactggaacc gaccagcgca ggaagtcagt
    36961 gcgttaatgg aactgtttgt gtgggcgctc gatagcgaag taaaaagtct gcaccgacat
    37021 aacgtgcgtc tgcgtattat tggcgatacc agtcgcttta actcgcgttt gcaagaacgt
    37081 attcgtaaat ctgaagcgct aacagccggg aataccggtc tgacgctgaa tattgcggcg
    37141 aactacggtg gacgttggga tatagtccag ggagtcaggc aactggctga aaaggtgcag
    37201 caaggaaacc tgcaaccaga tcagatagat gaagagatgc taaaccagca tgtctgtatg
    37261 catgaactgg cccctgtaga tttagtaatt aggactgggg gggagcatcg cattagtaac
    37321 tttttgcttt ggcaaattgc ctatgccgaa ctttacttta cagatgttct ctggcccgat
    37381 ttcgatgaac aagactttga aggggcgtta aatgcctttg ctaatcgaga gcgtcgtttc
    37441 ggcggcaccg agcccggtga tgaaacagcc tgatgggggt cgcttttgct gaagtatcgc
    37501 ctgatatctg cttttgtgtt aatacccgtc gtcatcgcgg cgttgtttct gttgccgccg
    37561 gtggggttcg ccattgtaac gctggtggtc tgcatgctgg cagcgtggga atggggacag
    37621 cttagcggtt ttaccactcg ttcgcagcga gtatggttgg cggtgttatg cgggttattg
    37681 ttggcgctga tgctttttct gttgccggaa tatcaccgaa atattcatca accgctggtt
    37741 gaaatctcac tttgggcttc gctgggttgg tggattgtcg cgctattgct ggtgctgttt
    37801 tacccaggtt ccgcagcaat ctggcgtaac tctaaaacat tgcgccttat ttttggcgtg
    37861 ctaaccattg ttcccttctt ctggggcatg ctggcgttac gggcctggca ctatgacgag
    37921 aatcattaca gtggcgcaat atggctgctc tatgtcatga tcctggtatg gggcgctgac
    37981 tccggcgcat atatgtttgg caaattgttt ggtaaacata agctggcacc gaaggtttct
    38041 ccgggtaaaa cctggcaagg ctttatcggt ggactcgcta ctgcagcggt aatctcatgg
    38101 ggttatggca tgtgggcgaa tctcgacgtc gctcccgtca ccttactcat ttgctctatt
    38161 gtcgcagcgt tagcctcagt gctcggcgat ctgaccgaga gtatgtttaa gcgtgaagca
    38221 ggaattaagg acagcggtca tttaattcca ggacacggtg gtattttaga tcgtattgat
    38281 agcctgacgg ctgcggtacc ggtctttgct tgcttgttgt tactggtatt caggacgctt
    38341 taacggaagg taatatgctg agttttctct gggatttggc ttcgttcatc gttgcactgg
    38401 gtgtacttat caccgtgcat gaatttggtc atttctgggt tgcccggcgt tgtggtgttc
    38461 gcgttgagcg tttctcaata gggtttggta aggcgctctg gcggcgaact gataagctcg
    38521 gcaccgaata tgttatcgcc ctgatcccgt tgggcggtta tgtcaaaatg ctggatgagc
    38581 gcgcagaacc ggtcgttccg gaactccgcc accatgcctt caataataaa tctgtcggcc
    38641 aacgagcggc gattattgcc gcaggtccgg ttgcaaactt catttttgct atctttgcct
    38701 actggctggt ttttattatt ggtgtgcctg gcgtacgtcc ggtggttggc gaaatagcag
    38761 ccaattcgat agctgcggaa gcacaaattg caccaggtac ggaactaaaa gccgtagatg
    38821 gtatcgaaac gcctgattgg gatgccgtgc gtttgcagtt ggtcgataaa attggcgatg
    38881 aaagcaccac cattacagta gcgccatttg gcagcgacca acggcgggat gtaaagctcg
    38941 atttacgtca ctgggcgttt gagcctgata aagaagatcc ggtatcttcg ctggggattc
    39001 gtcctcgtgg gccgcaaatt gaacctgtac tggaaaatgt gcagccaaac tcggcggcaa
    39061 gcaaggcagg tttgcaagca ggcgacagga tcgttaaagt cgatggtcag cccttaacgc
    39121 agtgggtgac ctttgtgatg cttgtccggg ataacccggg taaatcctta gcgttagaaa
    39181 tcgaaaggca ggggagtccc ttgtctttga cattaatccc ggagagtaaa ccgggtaatg
    39241 gtaaagcgat tggttttgtc ggtattgagc cgaaagtcat tcctttgcca gatgagtata
    39301 aagttgtacg ccagtatggg ccgttcaacg ccatcgtcga agccacggac aaaacgtggc
    39361 agctgatgaa gctgacggtc agtatgctgg gaaaattgat caccggtgat gtgaaactga
    39421 acaacctcag tgggccgatc tctatcgcca agggggctgg gatgacagcg gaactcgggg
    39481 tagtttatta cctgccgttt cttgcgctta ttagcgtgaa cttagggata attaacctgt
    39541 ttccgttgcc cgtacttgac ggggggcatc tgctgttcct tgcgatcgaa aagatcaaag
    39601 gcggaccggt atccgagcgg gttcaagact tttgttatcg cattggctcg attctgctgg
    39661 tgctgttaat ggggcttgca cttttcaatg atttctctcg gttatgagag ttagttagga
    39721 agaacgcata ataacgatgg cgatgaaaaa gttgctcata gcgtcgctgc tgtttagcag
    39781 cgccaccgta tacggtgctg aagggttcgt agtgaaagat attcatttcg aaggccttca
    39841 gcgtgtcgcc gttggtgcgg ccctcctcag tatgccggtg cgcacaggcg acacggttaa
    39901 tgatgaagat atcagtaata ccattcgcgc tctgtttgct accggcaact ttgaggatgt
    39961 tcgcgtcctt cgtgatggtg atacccttct ggttcaggta aaagaacgtc cgaccattgc
    40021 cagcattact ttctccggta acaaatcggt gaaagatgac atgctgaagc aaaacctcga
    40081 ggcttctggt gtgcgtgtgg gcgaatccct cgatcgcacc accattgccg atatcgagaa
    40141 aggtctggaa gacttctact acagcgtcgg taaatatagc gccagcgtta aagctgtcgt
    40201 gaccccgctg ccgcgcaacc gtgttgacct aaaactggtg ttccaggaag gtgtgtcagc
    40261 tgaaatccag caaattaaca ttgttggtaa ccatgctttc accaccgacg aactgatctc
    40321 tcatttccaa ctgcgtgacg aagtgccgtg gtggaacgtg gtaggcgatc gtaaatacca
    40381 gaaacagaaa ctggcgggcg accttgaaac cctgcgcagc tactatctgg accgcggtta
    40441 tgcccgtttc aacatagact ctacccaggt cagtctgacg ccagataaaa aaggtattta
    40501 cgtcacggtg aacatcaccg aaggcgatca gtacaagctt tctggcgttg aagtgagcgg
    40561 caaccttgcc gggcactccg ctgaaattga gcagctgact aagatcgagc cgggcgagct
    40621 gtataacggc accaaagtga ccaaaatgga agatgacatt aaaaagcttc tcggtcgcta
    40681 tggttatgcc tatccgcgcg tacagtcgat gcctgaaatt aacgatgccg acaaaaccgt
    40741 taaattacgc gtgaacgttg atgcgggtaa ccgtttctac gtgcgtaaga tccgttttga
    40801 aggtaacgat acctcgaaag atgccgtcct gcgtcgcgaa atgcgtcaga tggaaggtgc
    40861 atggctggga agcgatctgg tcgatcaggg taaggagcgt ctgaatcgtc tgggcttctt
    40921 tgaaactgtc gataccgata cccaacgtgt tccgggtagc ccggaccagg tagatgtagt
    40981 ctacaaggtg aaagaacgta acactggtag cttcaacttc gggattggtt acggtactga
    41041 aagtggcgtg agcttccagg ctggcgtgca acaggataac tggttaggta caggttatgc
    41101 tgttggtatc aacgggacca aaaacgatta ccagacctat gctgaactgt cggtaaccaa
    41161 cccgtacttc accgtagatg gcgtaagcct cggtggtcgt ctcttctata atgacttcca
    41221 ggcagatgac gccgacctgt ccgactatac caacaagagt tatggtacag acgtgacgtt
    41281 gggcttcccg attaacgaat ataactcgct gcgtgcaggt ctgggttatg tacataactc
    41341 cctgtccaac atgcagcctc aggttgcgat gtggcgttat ctgtactcta tgggtgaaca
    41401 tccgagcacc tctgatcagg ataacagctt caaaacggac gacttcacgt tcaactatgg
    41461 ttggacctat aacaagcttg accgtggtta cttcccgaca gatggttcac gtgtcaacct
    41521 gaccggtaaa gtgaccattc ctggatcgga taacgaatac tacaaagtga cgttagacac
    41581 ggcgacttat gtgccgatcg atgacgatca caaatgggtt gttctggggc gtacccgctg
    41641 gggttatggt gatggtttag gcggcaaaga gatgccgttc tacgagaact tctatgccgg
    41701 tggttccagc accgtgcgtg gcttccagtc caatacaatt ggtccgaaag cagtttactt
    41761 cccgcatcag gccagtaatt atgatccgga ctatgattac gaatgtgcga ctcaggacgg
    41821 cgcgaaagac ctgtgtaaat cggatgatgc tgtaggcggt aacgccatgg cggttgccag
    41881 cctcgagttc atcaccccga cgccgtttat tagcgataag tatgctaact cggttcgtac
    41941 ttccttcttc tgggatatgg gtaccgtttg ggatacaaac tgggattcca gccaatattc
    42001 tggatatccg gactatagtg atccaagcaa tatccgtatg tctgcgggta tcgcattaca
    42061 atggatgtcc ccattggggc cgttggtgtt ctcctacgcc cagccgttca aaaagtacga
    42121 tggagacaag gcagaacagt tccagtttaa catcggtaaa acctggtaag tgttctccac
    42181 aaaggaatgt agtggtagtg tagcgatgac tttaggcgat caatataaat cgccgggcca
    42241 cgcaaagaac tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg
    42301 gttattagct gcaggtctcg gtttagcact ggcaacatct gctcaggcgg ctgacaaaat
    42361 tgcaatcgtc aacatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa
    42421 cacgctggaa aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct
    42481 gcaggctaaa atgaaaaagc tgcagtccat gaaagcgggc agcgatcgca ctaagctgga
    42541 aaaagacgtg atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga
    42601 tcgcgcacgt cgttccaatg aagaacgcgg caaactggtt actcgtatcc agactgctgt
    42661 gaaatccgtt gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta
    42721 caacagcagc gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat
    42781 gccttcaatt cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg
    42841 cgatatcgtc atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt
    42901 catggttaac ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat
    42961 gacccaggac gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct
    43021 gacttacgcg cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc
    43081 acccagtgcg gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa
    43141 cgcggtgatt gaatccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt
    43201 cgtaggtaaa aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta
    43261 ccatgagatc cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga
    43321 cggctttggt tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt
    43381 aattattggc gatcgcgtgg agatcggtgc ctgcactacc atcgatcgcg gcgcgctgga
    43441 tgacactgtt attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt
    43501 cgtgattggc gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat
    43561 tggtcgttac tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga
    43621 caaagtgacg gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta
    43681 ttcctcaggc attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat
    43741 gaacattgat gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga
    43801 ctaacgttcc atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc
    43861 aggccgtgtt attattgtcg tttcttatat tttgacagga agagtatctt gactactaac
    43921 actcatactc tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta
    43981 ctggtggatc gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc
    44041 tctgtcaatg agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg
    44101 ctgattctgg aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa
    44161 ctggaaccgg gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg
    44221 gtcgtgcctg gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg
    44281 acccgtttta aaggggttgc tctggtcgac ggtaaagtag tttgcgaagc aacgatgatg
    44341 tgtgctcgta gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc
    44401 gccattgtgg aagagggcgc gtcgattggc gcgaacgcac acattggtcc tttttgtatc
    44461 gttggacccc atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat
    44521 ggtcatacta aaattggccg cgataatgag atttatcagt tcgcctccat cggcgaagtt
    44581 aaccaggatc tgaaatatgc tggcgaaccg acccgcgtgg aaatcggcga tcgtaaccgc
    44641 attcgcgaaa gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg
    44701 ggcagcgaca acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac
    44761 cgctgtattc tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg
    44821 atcatcggcg gcatggccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt
    44881 ggcggctgct ccggtgtggc gcaggacgtc cctccttatg tcattgcgca gggtaaccac
    44941 gccacgccgt tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg
    45001 attaccgcta tccgcaatgc gtataagctg atttatcgta gcggtaaaac gctcgatgaa
    45061 gtgaaaccgg aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat
    45121 ttctttgcac gctcaacgcg cggtctgatt cgttaatgac tgaacagcgt ccattaacga
    45181 ttgccctggt cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc
    45241 tgaaagaacg tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg
    45301 aaggctgcga agcctggtac gaaatggaag agttggcggt gatgggcatt gttgaagtgc
    45361 tcggtcgtct gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggtgaac
    45421 taaagccaga tgtttttgtt ggcattgatg cgcctgactt caatattatc ctcgaaggta
    45481 accttaaaaa gcagggtatc aaaaccattc attatgtcag tccgtccgtc tgggcgtggc
    45541 gacagaaacg cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt
    45601 tcgaaaaagc gttttatgac aaatacaacg taccgtgccg ctttatcggt cataccatgg
    45661 ctgatgccat gccattagat ccagataaaa atagtgcccg tgatgtgctg gggatccctt
    45721 acgatgccca ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta
    45781 gtgccgattt cctgaaaacg gcccagcttt tgcgccagac atatccggat ctcgaaatcg
    45841 tggtgccact ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgcagtcg
    45901 cgccagacct gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg
    45961 atgcggcact actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga
    46021 tggtggtggg atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa
    46081 ctgattatgt ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc
    46141 aggaagagtg tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaatggga
    46201 aaaccagcca cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg
    46261 ccgatgagca ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtctatcc
    46321 gcacacgcag ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt
    46381 cgtcaccgct gcggtgattc tcgacccggc gcgcccgatt gccgggctga atgattccaa
    46441 aaagctgagc gaaaaacgtc gtctggcgct ctgtgaagag atcaaagaga aagcgttgag
    46501 ctggagtctg ggccgcgcgg aacctcacga aatcgacgaa ctgaatattt tacatgcgac
    46561 catgctggcg atgcagcgag ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat
    46621 tgatggtaac cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggcgatag
    46681 ccgcgtaccg gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat
    46741 ggcggcgctg gatattgttt tcccgcaata tggttttgcc caacacaaag ggtatccaac
    46801 cgcttttcat ctggaaaaac tggctgaaca cggcgcgacc gaacaccatc ggcgcagctt
    46861 tgggcctgtc aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa
    46921 ccggaatctg aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc
    46981 gatgatcgat ggcctggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat
    47041 gccagcactg gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc
    47101 gggacatggc gcagggatta agcctatcgt cggggcagat tttaacgtcc agtgcgacct
    47161 gctgggtgat gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa
    47221 tctgacgttg ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat
    47281 cgatcgcgac tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat
    47341 gggcgacgtc ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc
    47401 gttttatgaa gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc
    47461 ggatgaagaa agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt
    47521 cgtggcgact aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg
    47581 cgtcgcgatc cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc
    47641 gcagcaatat atgcgtagcg aagaggagat gtgcgagctg tttgccgaca tcccagaagc
    47701 ccttgccaac accgttgaga tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata
    47761 cttcctgccg cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc
    47821 aaaagagggc ctggaagagc gtctggcctt tttattccct gatgaggaag aacgtgttaa
    47881 gcgccgcccg gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg
    47941 cttcccgggc tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt
    48001 accggtaggg ccaggccgtg gctccggtgc gggttcactg gtggcctacg cgctgaaaat
    48061 caccgacctc gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg
    48121 tgtctccatg cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga
    48181 acacgtagcg gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat
    48241 ggcggcgaaa gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt
    48301 cgatcgtatc tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga
    48361 agccgagccg cagctgccgg aaatctacga agcggatgaa gaagttaagg cgttgatcga
    48421 catggcgcgc aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt
    48481 tatcgcgccg accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca
    48541 tccggtcacc cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt
    48601 ccttggtttg cgtacgctca ccatcatcaa ctgggcgctg gagatgatca acaagcggcg
    48661 ggcgaagaat ggcgagccgc cgctggatat cgccgcgatc ccgctggatg acaagaaaag
    48721 cttcgacatg ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat
    48781 gaaggacctg atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc
    48841 actgttccgc cctggtccgt tgcaatcagg gatggtggat aactttatcg accgtaaaca
    48901 tggtcgcgaa gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaaccggt
    48961 actggagcca acctacggca ttatcctgta tcaggaacag gtcatgcaga ttgcgcaggt
    49021 gctttctggt tataccctcg gtggcgcgga tatgctgcgt cgtgcgatgg gtaagaaaaa
    49081 gccggaagag atggctaagc aacgttctgt atttgctgaa ggtgcagaaa agaacggaat
    49141 caacgccgaa ctggcgatga aaatcttcga cctggtggag aaattcgctg gttacggatt
    49201 taacaaatcg cactctgcgg cctatgcttt ggtgtcatat caaacattat ggttgaaagc
    49261 gcactatccg gcagagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa
    49321 ggtggtgggc ctggtggatg agtgctggcg gatggggctg aaaatcctgc caccagatat
    49381 aaactccggt ctttaccatt tccacgtcaa cgacgacggc gaaatcgtgt atggtattgg
    49441 cgcgatcaaa ggggtaggtg aaggtccgat tgaggcgatc atcgaagccc gtaataaagg
    49501 cggctacttc cgcgaactgt ttgatctctg cgcccgaacc gacaccaaaa agttgaaccg
    49561 tcgcgtgctg gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc cacatcgcgc
    49621 ggcgctgatg aactcgctgg gcgatgcgtt aaaagcggca gatcaacacg cgaaagcgga
    49681 agctatcggt caggccgata tgttcggcgt gctggccgaa gagccggaac aaattgaaca
    49741 atcctatgcc agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac
    49801 gttaggcctg tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta
    49861 tgtcggaggc gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacggc
    49921 tgcggggctc gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat
    49981 ctgcacgctg gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga
    50041 taaataccag catttgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt
    50101 tgatgacttc agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc
    50161 ccgggaaaaa tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca
    50221 gcttttaaac cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca
    50281 tctctactat cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt
    50341 ctctccgagc gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga
    50401 actggagttt gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc
    50461 gattgcagag ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa
    50521 actggatatt aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac
    50581 gcgtaaaatc ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccaca
    50641 gcgtccttat accctggatt acgttcgcct ggcatttgat gaatttgacg aactggctgg
    50701 cgaccgcgcg tatgcagacg ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg
    50761 tccggtgatg atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa
    50821 ctttggtatg ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga
    50881 acgctttaag atgcctatca tcacctttat cgacaccccg ggggcttatc cgggcgtggg
    50941 cgcagaagaa cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct
    51001 cggcgtaccg gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat
    51061 tggcgtgggc gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga
    51121 aggttgtgcg tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat
    51181 gggtatcatc gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc
    51241 gctgggcggc gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct
    51301 ggcggatctg gccgatctcg acgtgttaag cactgaagat ttaaaaaatc gtcgttatca
    51361 gcgcctgatg agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg
    51421 tggccctttt ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag
    51481 gaacacatga acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc
    51541 atcaaagaac tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac
    51601 agcgttgatt tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac
    51661 tgggatgaat acagtctcga tttatgtagc gatatcaatc agcttaatga atatctcccg
    51721 ctttatgcct tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg
    51781 gcgctctgtt tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt
    51841 cagtacaccg acgaatatct tgataacatt accccaccgt tcacgaaagc cttgtttacc
    51901 tacgtcaaag agcggaagta caccttttgt acgccgggtc atatgggcgg caccgcatat
    51961 caaaaaagcc cggttggctg tctgttttat gactttttcg gcgggaatac ccttaaggct
    52021 gatgtctcta tttcggtcac cgagcttggt tcgttgctcg accacaccgg gccacacctg
    52081 gaagcggaag agtacatcgc gcggactttc ggcgcggaac agagttatat cgttaccaac
    52141 ggaacatcga cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg cagtacgctg
    52201 ttgattgacc gcaattgtca taaatcgctg gcgcatctgt tgatgatgaa cgatgtagtg
    52261 ccagtctggc tgaaaccgac gcgtaatgcg ttggggattc tgggtgggat cccgcgccgt
    52321 gaatttactc gcgacagcat cgaagagaaa gtcgctgcca ccacgcaggc acaatggccg
    52381 gttcatgcgg tgattaccaa ctcaacctat gatggcttgc tctacaacac cgactggatc
    52441 aaacagacgc tggatgtccc gtcgatccac ttcgattccg cctgggtgcc gtacacccat
    52501 tttcatccga tctaccaggg gaaaagtggt atgagcggcg agcgtgttgc gggaaaagtg
    52561 atcttcgaaa cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggt ttcgctgatc
    52621 cacattaaag gtgagtatga cgaagaggcg tttaacgaag cctttatgat gcataccacc
    52681 acctcgccca gttatcccat tgttgcttcg gttgagacgg cagcagcgat gctgcgtggt
    52741 aatccgggca aacggctgat taaccgttca gtagaacgag ctctgcattt tcgcaaagag
    52801 gtccagcggc tgcgggaaga gtctgacggt tggtttttcg atatctggca accgccgcag
    52861 gtggatgaag ccgaatgctg gcccgttgcg cctggcgaac aatggcacgg ctttaacgat
    52921 gcggatgccg atcatatgtt cctcgatccg gttaaagtca ctattctgac gccggggatg
    52981 gacgagcagg gcaatatgag cgaggagggg atcccggcgg cgctggtggc gaagttcctc
    53041 gatgagcgtg ggatcgtggt agagaaaacc ggcccatata acctgctgtt tctctttagt
    53101 attggcatcg ataaaaccaa agcaatggga ttattgcgtg ggttgacgga atttaaacgc
    53161 tcttacgatc tcaatctgcg aatcaaaaat atgcttccgg atctgtacgc agaagatccc
    53221 gatttctacc gcaatatgcg tattcaggat ctggcgcaag ggatccataa gctgattcgt
    53281 aaacacgatc ttcccggttt gatgttgcgg gcattcgata ctttgccgga gatgatcatg
    53341 acgccacatc aggcatggca gagacagatt aaaggtgaag tagaaaccat tgcgctggaa
    53401 caactggtcg gtagagtatc ggcaaatatg atcctgcctt atccaccggg cgtaccgctg
    53461 ttgatgcctg gagaaatgct gaccaaagag agccgcacag tactcgattt tctactgatg
    53521 ctttgttccg tagggcaaca ttaccccggt tttgaaacgg atattcacgg tgcgaaacag
    53581 gacgaagacg gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact tgccagagcg
    53641 gcttccgggc gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac
    53701 aggttcacca tattgcgatt attgcgacgg attatgcggt gagcaaagct ttctactgcg
    53761 atattcttgg tttcacgctg caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag
    53821 gggatttggc gcttaatggg caatatgtga ttgagctttt ctcatttccg ttcccgccgg
    53881 agcgccccag ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca
    53941 tcgatgcggc agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg
    54001 atccatacac gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg ccgttggaac
    54061 tgtatgagca gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg ggcattgcta
    54121 ctgtaaaatc gcaccatcat gacactcacg ctcaatagac aacttctcac ctcacgccag
    54181 attctggtgg cctttagcgg cgggcttgac tccaccgttc tgctgcatca gttggtgcag
    54241 tggcggacgg aaaatccggg gggcgctctg cgcgctattc atgtgcatca cggtttaagt
    54301 gccaatgccg atgcctgggt tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg
    54361 ctggtggtcg aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc ccaggcgcgg
    54421 caggcgcgtt atcaggcatt tgcccgcacc ttgttgcccg gtgaagtgct ggtcaccgcg
    54481 caacatctcg acgatcaatg tgaaactttt ctgctggcgc taaaacgcgg tagcggccct
    54541 gccgggcttt cggctatggc ggaagtttcg gagtttgccg gaacacggct tatccgcccg
    54601 ttgcttgccc gcacgcgggg ggaactggcg cagtgggcat tggcgcatgg tttacgctgg
    54661 attgaagacg aaagtaatca ggacgactca tacgatcgta actttctgcg cctgcgcgta
    54721 gtgccgttat tgcagcagcg ttggccgcat tttgccgaaa caacggcccg cagcgccgca
    54781 ctttgtgctg aacaagagag cctgctggat gaactgctgg cagatgattt agcacactgt
    54841 caatcgccgc aggggacgct gcagattgtg ccaatgctgg cgatgagtga tgcccgccgc
    54901 gcggcgatta tccgccgctg gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg
    54961 ttggtgagga tctggcagga agtggcgctg gcgcgggaag atgcctcccc ctgtttacgt
    55021 ttgggcgcgt ttgaaatccg acgctatcag tcgcaactgt ggtggattaa atccgtcacc
    55081 gggcaaagcg aaaacattgt gccgtggcag acatggcttc aaccgctgga attaccggcg
    55141 gggcagggaa gtgtacagct taatgcggga ggcgatattc gccctccgcg tgcagacgaa
    55201 gcggtcagcg tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg taacggcgga
    55261 cgtaagctaa agaaaatctg gcaagagctg ggcgtgccgc cgtggctacg tgacaccacg
    55321 ccactgctgt tttatggcga aacgctgatt gcggcggcag ggggatttgt gacgcaagaa
    55381 ggtgtggctg aaggtgagaa tggcataagt tttgtctggc agcgaaacgc ttagtttagt
    55441 gaaggccgga tatgacgcat taagcgtcgc atccggcgaa agtcaatcag gactcgctca
    55501 ccaccaccgt accgatttcc gggtggctaa agctggtaat tttatccaga cgtagctcac
    55561 gggtttcgcc agcggcctcg acgaccagat actccacatt tttgcgggag actaaatcgc
    55621 tggctttggc ctgcaatttt tcgccatctt tcagctcaag tgtcagcatt aaatgatgct
    55681 ggcaggcgag ctcaagatta tcgtaatcat cacaattgat tggttgatac gtatcattca
    55741 ttgacataat cgctcaccag taagtttgcc gcagcgtatg ctgctttttc cctgacagcc
    55801 tcagaaaggg cgtcgtcggc agccatttca ttcagcactt tcaaaacgca gcccagcgcg
    55861 tccggaacgt atcctaagtc tccgctggcg atttccgcgt accgcttgcg tattaactca
    55921 caatattttt ccacatgccc tcctgtcagc actctgactt aaccgtggat gcaagtctaa
    55981 gcctacgaag ataaactctg tttcgcaagg tgactatacc acactcattt ctgcaatatc
    56041 agcgccgcaa ctgcacgtat tccgttacaa tggcctcctg attcgaaagg agttttctta
    56101 tggcgcttaa agcgacaatt tataaagcga cggttaatgt ggccgatctc gaccgcaacc
    56161 agtttctcga tgcctctctg acgctggcgc gccacccttc agaaacccag gagcgtatga
    56221 tgctgcgcct gctggcgtgg ctgaaatatg ctgatgaacg tctgcaattt acccgtggtt
    56281 tgtgtgccga tgatgagccg gaagcgtggc tgcgtaacga tcacctgggc attgatttgt
    56341 ggattgagct ggggctgccg gatgagcgac gaattaagaa agcctgcacc caggccgcag
    56401 aagtggcgct gtttacctat aatagtcggg cggcgcaaat ctggtggcag caaaatcaga
    56461 gcaaatgtgc gcagtttgcc aatctttccg tctggtatct ggacgacgag cagctggcga
    56521 aagtgagcgc ctttgccgat cgtaccatga cgctgtaggc aacgattcag gatggcgtga
    56581 tctggttatc ggatgataag aataatctgg aagtgaactt aaccgcctgg caacaacctt
    56641 catgattgtg atttcccgac atgttgctat tcccgatggt gagcttgaga tcaccgccat
    56701 tcgtgcgcag ggcgcgggcg ggcagcatgt taataagacc tcaacggcca ttcatctgcg
    56761 ttttgacatt cgggcatcca gcctgccaga gtattacaaa gagcgtctgc tcgccgccag
    56821 ccatcatttg atcagcagtg atggcgtaat tgtcattaag gcacaggaat accgcagtca
    56881 ggaactgaac cgcgaagcgg cgctggcccg gctggtggca gtgattaaag atttaacaac
    56941 agaacaaaaa gcccgacgac ccacgcggcc cacccgtgca tcgaaagagc gcaggctggc
    57001 atcgaaagca caaaaatcaa gcgtgaaggc gatgcgcagc ggtcgggaat aaaaagaagg
    57061 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57121 tgggttgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaattga
    57181 aaccgatgcc gcaaagttgg cgcggcgtgc tgccgtgtgc cgattgcgaa ggaatcgaaa
    57241 cctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57301 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57361 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57421 tgctcgatcg tgaaggcaat ccgattgaat cgcagttcaa ctatacgctg gaaccggcac
    57481 aatccagttt acccatgacg ccgatgaccc tgcggggcat gtatttttat atggctgatg
    57541 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57601 tggagcgtgg ctacctggct gcgcgcggcc acagtgaaaa accggtgtta ctgtcagtag
    57661 aaggtcactt tacgcttgag gctaatccgg ataccggtgc gccaaccaaa gtattagcac
    57721 ccgatacggc agggaaattt tacccaaacc aggattgcag tagtttgggg cagtaacccg
    57781 tcttgagaca gaaacaaacg caaaacaggc cagaaggata tatttcaaca ttttgaattt
    57841 gcacgttttt tgtaggccgg ataaggcgtt tacgccgcat ccggcaatgg tgctcaacgc
    57901 ctgatgcgac gctggcgcgt cttatcatgc ctacaacccc cctcatacct taaggcggat
    57961 aaggcattta ctttatcacc gggtcaaacc aacaaagcga cccgccttga tataaatccc
    58021 tggcttcagg tgcccaacat actgtaactg cgtttctggc ttaaacgcag agacatcacc
    58081 aatacgcata tgcatcagat cggcagggct aatccagccc gattgcgcca gcgtaagcgg
    58141 atgcccggct ttggcaaatg catcagtgac aaattccgaa caaaaccacg actttttgtc
    58201 tccttcgacc acactgctta actgcgcttt cgccaggccg ctgacgcact gttggcgaaa
    58261 atcctcggag aacggattca gtgagcacat ctggcgagtc accataaagg gaataaattc
    58321 gacaatgccg cgatagttat aaccgctatc tttgatttta ttggcgaacg cggtgatttc
    58381 tgtggcttgt tgcggggtaa gatccgggac tcgtaagacg aaaagtttat cactatgctt
    58441 catcgctttt ttaagggaaa caatctggac gccagcgcct gtcgcttctg caacgttatt
    58501 ttcacccaga tagattgcaa cgtgactcac agaggaagtg ctgaaaacgc ggatcccaaa
    58561 tgaggttacc tccaggcttg aggagaacag caaatcgccg ggtttgagat caggtaccgt
    58621 tatttctttt attgattgtt cggtgaaaga gctttgatgc tggaatttga cagcccatgt
    58681 tttcgcctcg gcatctactg ctgtggctga tgagtctggc tggctgatat caacggtaca
    58741 ggccgaaagt aaaagaaagc aggggagaag caggcggcag tacgcctttg gtttatccat
    58801 tttatacaat ccatgtaaaa aagggccctg aaattcagga ccctttctgg catcagcctt
    58861 taatctgttt caccagatat tcgacgatgt caccagtctt aattaactgt ttctcgccgt
    58921 tgcgacgata tttatattcg atatcgtcgt tgtcgaggtt acggtcgccc agcacaatag
    58981 tgtgcggaat accgatcagt tccatatcgg caaacatcac gcccggacgt tcttttcggt
    59041 catccagcag cacttcgata ccttgtgcac gcagttcgct gtacagtttc tcagcaagct
    59101 cttgtacgcg gaaggatttg tgcatgttca tcggcagaat tgccacctgg aacggcgcga
    59161 tagcgtcagg ccatacgatg ccgcgctcgt cgtagttctg ctcaatcgcc gcagccacta
    59221 cgcgcgttac cccgataccg tagcaaccca tagtcaggat ttggttacgg ccatcttcac
    59281 cctgtacgga ggctttcagt gcttcggagt acttggtacc cagctggaag atgtgaccaa
    59341 cttcgatacc acgtttgatc agcagcgtac cctggccatc cgggcttgga tcgccagcca
    59401 ccacgttacg gatatccgca acttccgggg tagcgacatc gcgatcccag ttgatgccga
    59461 agtagtgttt accatcgatg ttagcaccag cagtgaaatc actcatcgcc gcaacggtac
    59521 ggtcaatcac caccggaatc ggcatgttta ccggacccag tgaacccgga ccggctttaa
    59581 ccacggcacg aatttcttct tcggtcgcga aagtcagcgg gctggcaacc tgcggcagtt
    59641 tttctgcttt aacttcgttc agctcgtgat caccgcgcac cagcagcgca accagcggga
    59701 agctactgcc ttcaaccgct ttaaccagca gagtcttaac cgttttctta atcggcagat
    59761 tgaactgttc aaccagttcc gcgatggttt tcgcgttcgg cgtatcaacc agcgtcattt
    59821 cctgggtagc agcagcgcgc ggttctttcg gcgcgatagc ttctgccagc tcaatgttcg
    59881 ctgcatagtc agaggtgtcg gagaagacca catcgtcttc accgctctgc gccagcacct
    59941 ggaattcgtg agaggcgctg ccgccgatag aaccggtgtc ggcctgtacg gcgcggaaat
    60001 ccagccccat gcggctgaag attttgctgt aggccgcata cattgcatcg taggtttcct
    60061 gcagggattc ctgagaagta tggaaagagt aagcatcttt catcaggaat tcgcgggaac
    60121 gcatgacgcc gaaacgcgga cgcacttcgt cgcggaactt ggtctggatc tgatagaagt
    60181 tcagcggcag ctgtttgtaa gagctaagct cgttacgaat caggtcagtg ataacttctt
    60241 catgagttgg gccgagtacg aacggacgct cgccacggtc aacaaaacgc agcagttccg
    60301 gaccgtactg ttcccaacga ccactctctt gccacaaatc ggctggctga accaccggca
    60361 tcgacacctc gattgcaccg gcgttgttca tctcttcacg cacgatgttt tcgacttttt
    60421 tcagaacgcg cacgccggtc ggcagccagg tatataaccc ggaggccagc ttgcggatca
    60481 tcccggcgcg cagcatcagc tgatggctga tcacctcggc gtcggcaggt gtctccttga
    60541 gagtggagag caggtattgg ctagtacgca tgttgttacg gttccagttg gaaggtagaa
    60601 caggctcaag gcgagcctgg gacaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg
    60661 tcaaaagaga agggcgtgaa tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc
    60721 ggtgacgcgc cagcgaacgt taaaatcatg tagccagacg gcataggttt tgcccgtttc
    60781 ctcaccttta cgataggccg ggcgcgggtc ctgcgccagt acttcgcgga taaacagcgt
    60841 taactgcgga taacgcttct ccagcgtcaa aagctgcttt tcgacctctg cggtaaaact
    60901 caccgccatc tctgcagctg gcgcactttg cgcatagctg gcactggcat cgggaagcga
    60961 ttcggcaaag gggagatacg gtttgatatc cactaccggc gtaccatcga ccagatccag
    61021 gctgccgagc ttcagaatca cgcagtcttt atggcaaaca acctctttca gctctaccag
    61081 cgacatacca atcgggttgg ggcggaaagt ggagcgcgta gcgaaaacac ccattctggc
    61141 attaccgcca agacgaggcg gacgcacagt cggacgccag ccgccttcca tcgtttgatg
    61201 aaagacgaaa aggatccata aatggctgaa tgcttccagg ccgcgaacgg cgtcagcctg
    61261 gttgtaggga gcaatgagat gtagttcgcc gttggcgctt tttaccagac ctggctggcg
    61321 cggaacggcg aatttttctt tatagggcga gcgaataaca cctatttgct cgaactggaa
    61381 actgctcatt tcgccgtaat gttaagcgca gaaccgatac ataaagcctg acgatagcag
    61441 cctggcgtac cgctggtaac ttcgcagcta tgcagtaata ccgcattggc tttcattttc
    61501 gaggcgttga tttgcatccg cttacgcgcg gttggaatgc tcggcggaga gtcctgatta
    61561 gaggcctggc aagagtcgcc actgacttca ccgagatcgc ggaacggttt gccgactaat
    61621 tcttctgcat tggtataaat tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc
    61681 tccgctttcg gctggggtgc agtgctttga acgggttcga caggggatct gcttaacatg
    61741 gaacagccgc ttagcatgag tgctactaaa cagatcggta aagcacgcat agtatttcct
    61801 caatgtatga tcaaaacgtc aatattgaat caggagcttg taaaaatgac aagacgggca
    61861 agcgcccgtc ctgaatgata ttacaaattg tggaaacagc ctaaaaatta ccagccttta
    61921 acagcgccgc cgttaaacac tttgtttgct gcttcgtaaa cttcgtcaga ctgataagcc
    61981 tggacgaatt tcttcacgtt ctcggcgtct ttgttatctt cacgcgttac gatcaggttt
    62041 acgtacgggg agtctttatc ttcgacaaag ataccgtttt tcgctggagt caggccaatc
    62101 tggctggcat aggtggtgtt aataactgcc agagcgattt gcgcgtcgtc cagagaacgc
    62161 ggcagttgcg gtgcttccag ttcaacaatt ttcagatttt ttgggttctc aacaacatca
    62221 agaacggtcg gcagcaggcc aacgccatct ttcagtttga tcaagcccac tttttgcagc
    62281 agcagcagtg aacgaccaag gttagttgga tcgtttggca cggcaacctg cgaaccatcc
    62341 tgcagttcat ccagtgattt gattttcttg gagtaaccag caatcggata aacaaaagtg
    62401 ttgcctactg cgaccagttt gtagccacga tctttcagtt gctgatcaag gtacggttta
    62461 tgctggaagg cgttggcgtc gatatcgcct ttgctcaatg cttcgtttgg cagaacatag
    62521 tcgttgaagg ttaccagctc aacgtccagg ccatatttgt ctttcgcaac tttctgcgcg
    62581 acttctgcaa cctgctgttc ggcaccaaca atcacgccga ctttaatgtg gtttggatct
    62641 ttttcatcct gaccgcagcc taccagtgcc agtgatccaa tcagggctcc cactgccgca
    62701 aaggttttga atttgaacgc cataccttat tccttcttca attatttatg ttgtgttgaa
    62761 cgttacttgc gagtgacagc ccggacgatg cggtcgcctg cgaactgaat taaataaacc
    62821 agaatgacca gcaataccag taccgtattc atcaccgtcg cgttatagcc gatgtagcca
    62881 tactgatagc caatctgacc taaaccaccg gcaccgactg caccacccat cgcggaataa
    62941 ccgaccaggg taatcagggt gatagttgcc gcattcacca gacccggcaa cgcttccggt
    63001 aacagcactt tacggacgat ctgcattggc gtggcaccca ttgcgcggga agcttcaatt
    63061 aacccggttg ggatctccag cagagcgttc tcgaccatac gggcaataaa cggtgctgca
    63121 ccaacggtta acggaacaat tgctgcctgc aaaccaatcg atgtaccgac aataacgcgg
    63181 gtaaacggaa tcatccatac cagcaagata atgaacggga tggaacggaa aatgttcaca
    63241 atcgcagaaa cggtacgata cagcttcgcg ttagcaataa tttgccccgg acgcgtgaca
    63301 taaagcagga cgccaaccgg cagaccaacc acaaacccaa aaaatccgga tacgaaggtc
    63361 attgccagcg tttcccatac gccacgaacc agcagccaca tcatcggctc agacataacc
    63421 cagtacctct acttttacat ggtgttcctg cagccaggca atggcggctt gcgtatcttg
    63481 ttgtgtgccg tgcatttcag tcagcatgat gccgaactta acgccaccgg cgtaatccat
    63541 ctgcgcgcta ataatgttgt tgttgacgtt gaaacgacgc gcggtttcag aaagcagtgg
    63601 ggcatcgacc gattgaccgg taaactccag acgcagcatc ggcacgcagt cagtaaatgg
    63661 ctccgcttgc agacgttcct ggtaatcttc cgggatatcc agatgcaggg ttgactgaat
    63721 aaacttctgc gccagcggcg ttttcggatg tgagaacact tcacttaccg tgtcctgctc
    63781 gatcagttcg ccattgctga tgaccgcaac gcaatcacaa atacgcttca caacgtccat
    63841 ttcgtgggtg atcaacagaa tcgtcaaacc cagacggcgg ttaatgtctt tcagcagttc
    63901 gagaatagaa cgtgtcgttg ccgggtctag cgcgctggtg gcttcatcac acagcaatac
    63961 tttgggattg ctggctaatg cacgggcaat cgccacacgt tgtttctgcc caccggaaag
    64021 attcgacggg tagctatcat gcttatctcc aagaccaacc aatgacagca attccgtcac
    64081 gcgacgtttg atctcgtctt tcggtgtatt gtccagctcc agcggcagag ccacgttgcc
    64141 aaaaacagta cgcgaagaga gcaggttaaa atgctggaaa atcataccaa tctggcggcg
    64201 agctttggtc aactcggatt ctgacagcgt ggtcagttcc tgtccatcga ccagcacgct
    64261 accctcggtt gggcgctcca gcaggtttac acaacgtata agcgtactct tacccgcgcc
    64321 tgaggcaccg ataacgccat aaatttgtcc agctggcaca tgcaggctga cgttgttcaa
    64381 cgcctggatg gtgcgggtgc cctggtggaa cactttggtg atattcgaaa gttttatcat
    64441 tgattattta ttatcgtcat taagttagtc gtggcatctc gaatgcctga aacgggcaac
    64501 gccgtcaatg aaatggatgt taaggcatcc agacgtctaa atcaatcagg tttatgcgaa
    64561 gagcactttc ttgcaggtcg aaacatgcga tactagcgtc acatgcctta ttaaggagct
    64621 ataaaaggtg gcgaagagcg tacccgcaat ttttcttgac cgtgatggca ccattaatgt
    64681 cgatcacggc tatgtccatg agatcgacaa ctttgaattt atcgacggtg ttattgacgc
    64741 catgcgcgag ctaaaaaaaa tgggctttgc gctggtggta gtcaccaacc agtctggcat
    64801 tgctcgcggt aaatttaccg aagcacagtt tgaaacgctg accgagtgga tggactggtc
    64861 gctggcggac cgagatgtcg atctggatgg tatctattat tgcccgcatc atccgcaggg
    64921 tagtgttgaa gagtttcgcc aggtctgcga ttgccgcaaa ccacatccgg ggatgctttt
    64981 gtcagcacgc gattatttgc atattgatat ggccgcttct tatatggtgg gcgataaatt
    65041 agaagatatg caggcagcgg ttgcggcgaa cgtgggaaca aaagtgctgg tgcgtacggg
    65101 taaacctatt acacctgaag cagaaaacgc ggcagattgg gtgttaaata gcctggcaga
    65161 cctgtcgcaa gcgataaaaa agcagcaaaa accggcgcaa tgattaaaag atgagcggtt
    65221 gaaataaaaa tgcatttttc cgcttgtctt cctgagccga ctccctataa tgcgcctcca
    65281 tcgacacggc gaatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaaat
    65341 cctgaaattc agggttgact ctgaaag
//
LOCUS       DAZKIF010000009        57642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZKIF010000009 DAZKIF010000000
VERSION     DAZKIF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.009"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            36..224
                     /gene="dicB"
                     /locus_tag="ACHOTW_000643"
     CDS             36..224
                     /gene="dicB"
                     /locus_tag="ACHOTW_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9842812.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            221..412
                     /gene="ydfD"
                     /locus_tag="ACHOTW_000644"
     CDS             221..412
                     /gene="ydfD"
                     /locus_tag="ACHOTW_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9842813.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            505..2976
                     /locus_tag="ACHOTW_000645"
     CDS             505..2976
                     /locus_tag="ACHOTW_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9842814.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEGAASFVPVETESDPM
                     NVAFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RVHRQHTENSAEKIPPVTAVIRREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSEQ
                     PEPVKVTADEVKKIMQAANISQPDAEELLAVSRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCG
                     AVMGDATYQEIFDGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPL
                     IKANGHHNLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LDTAGGVIDRDTMKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWG
                     NGANFDNTILRRSYERQGSPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSAIWQKLIPSQADS"
     gene            3049..3300
                     /locus_tag="ACHOTW_000646"
     CDS             3049..3300
                     /locus_tag="ACHOTW_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9842815.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            3335..3522
                     /locus_tag="ACHOTW_000647"
                     /pseudo
     CDS             3335..3522
                     /locus_tag="ACHOTW_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(3994..4080)
                     /locus_tag="ACHOTW_000648"
                     /pseudo
     CDS             complement(3994..4080)
                     /locus_tag="ACHOTW_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4286..4612)
                     /gene="ynfA"
                     /locus_tag="ACHOTW_000649"
     CDS             complement(4286..4612)
                     /gene="ynfA"
                     /locus_tag="ACHOTW_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP9842816.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            4747..5088
                     /gene="ynfB"
                     /locus_tag="ACHOTW_000650"
     CDS             4747..5088
                     /gene="ynfB"
                     /locus_tag="ACHOTW_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP9842817.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            5123..5683
                     /gene="speG"
                     /locus_tag="ACHOTW_000651"
     CDS             5123..5683
                     /gene="speG"
                     /locus_tag="ACHOTW_000651"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP9842818.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5686..6396)
                     /gene="ynfC"
                     /locus_tag="ACHOTW_000652"
     CDS             complement(5686..6396)
                     /gene="ynfC"
                     /locus_tag="ACHOTW_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP9842819.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6504..6809
                     /gene="ynfD"
                     /locus_tag="ACHOTW_000653"
     CDS             6504..6809
                     /gene="ynfD"
                     /locus_tag="ACHOTW_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP9842820.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            7008..9434
                     /gene="ynfE"
                     /locus_tag="ACHOTW_000654"
     CDS             7008..9434
                     /gene="ynfE"
                     /locus_tag="ACHOTW_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFP9842821.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            9495..11918
                     /gene="ynfF"
                     /locus_tag="ACHOTW_000655"
     CDS             9495..11918
                     /gene="ynfF"
                     /locus_tag="ACHOTW_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP9842822.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            11929..12546
                     /gene="ynfG"
                     /locus_tag="ACHOTW_000656"
     CDS             11929..12546
                     /gene="ynfG"
                     /locus_tag="ACHOTW_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP9842823.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            12548..13402
                     /gene="ynfH"
                     /locus_tag="ACHOTW_000657"
     CDS             12548..13402
                     /gene="ynfH"
                     /locus_tag="ACHOTW_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP9842824.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVSGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            13445..14059
                     /gene="dmsD"
                     /locus_tag="ACHOTW_000658"
     CDS             13445..14059
                     /gene="dmsD"
                     /locus_tag="ACHOTW_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP9842825.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14217..15509
                     /gene="clcB"
                     /locus_tag="ACHOTW_000659"
     CDS             14217..15509
                     /gene="clcB"
                     /locus_tag="ACHOTW_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP9842826.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(15462..16157)
                     /gene="bioD"
                     /locus_tag="ACHOTW_000660"
     CDS             complement(15462..16157)
                     /gene="bioD"
                     /locus_tag="ACHOTW_000660"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9842827.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16282..17502)
                     /gene="mlc"
                     /locus_tag="ACHOTW_000661"
                     /gene_synonym="dgsA"
     CDS             complement(16282..17502)
                     /gene="mlc"
                     /locus_tag="ACHOTW_000661"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP9842828.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(17637..18530)
                     /gene="ynfL"
                     /locus_tag="ACHOTW_000662"
     CDS             complement(17637..18530)
                     /gene="ynfL"
                     /locus_tag="ACHOTW_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9842829.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            18637..19890
                     /gene="ynfM"
                     /locus_tag="ACHOTW_000663"
     CDS             18637..19890
                     /gene="ynfM"
                     /locus_tag="ACHOTW_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9842830.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(19939..20058)
                     /locus_tag="ACHOTW_000664"
     CDS             complement(19939..20058)
                     /locus_tag="ACHOTW_000664"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP9842831.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            20314..20622
                     /gene="asr"
                     /locus_tag="ACHOTW_000665"
     CDS             20314..20622
                     /gene="asr"
                     /locus_tag="ACHOTW_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP9842832.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            20715..20798
                     /locus_tag="ACHOTW_000666"
     CDS             20715..20798
                     /locus_tag="ACHOTW_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9842833.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            20898..21719
                     /gene="ydgD"
                     /locus_tag="ACHOTW_000667"
     CDS             20898..21719
                     /gene="ydgD"
                     /locus_tag="ACHOTW_000667"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP9842834.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(21758..22087)
                     /gene="mdtI"
                     /locus_tag="ACHOTW_000668"
     CDS             complement(21758..22087)
                     /gene="mdtI"
                     /locus_tag="ACHOTW_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP9842835.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(22074..22439)
                     /gene="mdtJ"
                     /locus_tag="ACHOTW_000669"
     CDS             complement(22074..22439)
                     /gene="mdtJ"
                     /locus_tag="ACHOTW_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP9842836.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            22851..23885
                     /gene="tqsA"
                     /locus_tag="ACHOTW_000670"
     CDS             22851..23885
                     /gene="tqsA"
                     /locus_tag="ACHOTW_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFP9842837.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(23910..25298)
                     /gene="pntB"
                     /locus_tag="ACHOTW_000671"
     CDS             complement(23910..25298)
                     /gene="pntB"
                     /locus_tag="ACHOTW_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP9842838.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(25309..26841)
                     /gene="pntA"
                     /locus_tag="ACHOTW_000672"
     CDS             complement(25309..26841)
                     /gene="pntA"
                     /locus_tag="ACHOTW_000672"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP9842839.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            27365..28309
                     /gene="ydgH"
                     /locus_tag="ACHOTW_000673"
     CDS             27365..28309
                     /gene="ydgH"
                     /locus_tag="ACHOTW_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP9842840.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            28495..29877
                     /gene="ydgI"
                     /locus_tag="ACHOTW_000674"
     CDS             28495..29877
                     /gene="ydgI"
                     /locus_tag="ACHOTW_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9842841.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            29914..30636
                     /gene="folM"
                     /locus_tag="ACHOTW_000675"
     CDS             29914..30636
                     /gene="folM"
                     /locus_tag="ACHOTW_000675"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP9842842.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(30633..30968)
                     /gene="ydgC"
                     /locus_tag="ACHOTW_000676"
     CDS             complement(30633..30968)
                     /gene="ydgC"
                     /locus_tag="ACHOTW_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP9842843.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            31097..31816
                     /gene="rstA"
                     /locus_tag="ACHOTW_000677"
     CDS             31097..31816
                     /gene="rstA"
                     /locus_tag="ACHOTW_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP9842844.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            31820..33121
                     /gene="rstB"
                     /locus_tag="ACHOTW_000678"
     CDS             31820..33121
                     /gene="rstB"
                     /locus_tag="ACHOTW_000678"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP9842845.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            33197..34126
                     /gene="tus"
                     /locus_tag="ACHOTW_000679"
     CDS             33197..34126
                     /gene="tus"
                     /locus_tag="ACHOTW_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP9842846.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(34123..35526)
                     /gene="fumC"
                     /locus_tag="ACHOTW_000680"
     CDS             complement(34123..35526)
                     /gene="fumC"
                     /locus_tag="ACHOTW_000680"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP9842847.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(35669..37315)
                     /gene="fumB"
                     /locus_tag="ACHOTW_000681"
     CDS             complement(35669..37315)
                     /gene="fumB"
                     /locus_tag="ACHOTW_000681"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9842848.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            37514..38689
                     /gene="manA"
                     /locus_tag="ACHOTW_000682"
     CDS             37514..38689
                     /gene="manA"
                     /locus_tag="ACHOTW_000682"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP9842849.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            38790..40298
                     /gene="ydgA"
                     /locus_tag="ACHOTW_000683"
     CDS             38790..40298
                     /gene="ydgA"
                     /locus_tag="ACHOTW_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9842850.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(40343..41607)
                     /gene="uidC"
                     /locus_tag="ACHOTW_000684"
                     /pseudo
     CDS             complement(40343..41607)
                     /gene="uidC"
                     /locus_tag="ACHOTW_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(41646..43019)
                     /gene="uidB"
                     /locus_tag="ACHOTW_000685"
     CDS             complement(41646..43019)
                     /gene="uidB"
                     /locus_tag="ACHOTW_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP9842851.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(43016..44829)
                     /gene="uidA"
                     /locus_tag="ACHOTW_000686"
                     /pseudo
     CDS             complement(43016..44829)
                     /gene="uidA"
                     /locus_tag="ACHOTW_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            complement(45220..45810)
                     /gene="uidR"
                     /locus_tag="ACHOTW_000687"
     CDS             complement(45220..45810)
                     /gene="uidR"
                     /locus_tag="ACHOTW_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP9842852.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(46039..46806)
                     /gene="hdhA"
                     /locus_tag="ACHOTW_000688"
     CDS             complement(46039..46806)
                     /gene="hdhA"
                     /locus_tag="ACHOTW_000688"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP9842853.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(46918..47946)
                     /gene="malI"
                     /locus_tag="ACHOTW_000689"
     CDS             complement(46918..47946)
                     /gene="malI"
                     /locus_tag="ACHOTW_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP9842854.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            48121..49713
                     /gene="malX"
                     /locus_tag="ACHOTW_000690"
     CDS             48121..49713
                     /gene="malX"
                     /locus_tag="ACHOTW_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP9842855.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            49723..50895
                     /gene="malY"
                     /locus_tag="ACHOTW_000691"
     CDS             49723..50895
                     /gene="malY"
                     /locus_tag="ACHOTW_000691"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP9842856.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            50999..52000
                     /gene="add"
                     /locus_tag="ACHOTW_000692"
     CDS             50999..52000
                     /gene="add"
                     /locus_tag="ACHOTW_000692"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9842857.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(52036..53076)
                     /gene="ydgJ"
                     /locus_tag="ACHOTW_000693"
     CDS             complement(52036..53076)
                     /gene="ydgJ"
                     /locus_tag="ACHOTW_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9842858.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            53319..53444
                     /gene="blr"
                     /locus_tag="ACHOTW_000694"
     CDS             53319..53444
                     /gene="blr"
                     /locus_tag="ACHOTW_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP9842859.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTMVQHK"
     gene            53717..53932
                     /gene="ydgT"
                     /locus_tag="ACHOTW_000695"
     CDS             53717..53932
                     /gene="ydgT"
                     /locus_tag="ACHOTW_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP9842860.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGLVPKSVWHYVQ"
     gene            54018..54458
                     /gene="ydgK"
                     /locus_tag="ACHOTW_000696"
     CDS             54018..54458
                     /gene="ydgK"
                     /locus_tag="ACHOTW_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP9842861.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            54535..55116
                     /gene="rsxA"
                     /locus_tag="ACHOTW_000697"
     CDS             54535..55116
                     /gene="rsxA"
                     /locus_tag="ACHOTW_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP9842862.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            55116..55694
                     /gene="rsxB"
                     /locus_tag="ACHOTW_000698"
     CDS             55116..55694
                     /gene="rsxB"
                     /locus_tag="ACHOTW_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP9842863.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            55687..>57642
                     /gene="rsxC"
                     /locus_tag="ACHOTW_000699"
     CDS             55687..>57642
                     /gene="rsxC"
                     /locus_tag="ACHOTW_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP9842864.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARLARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPRK"
BASE COUNT        14433 a        14746 c        14125 g        14338 t
ORIGIN      
        1 accgcatcac aaaattcatt gttgaggacg cgataatgga aacgttatta ccaaacgtta
       61 atacgtctga aggttgtttt gaaattggtg tcactatcag taaccctgta tttactgaag
      121 atgccattaa caagagaaaa cacgaacggg agttattaaa taaaatatgc attctttcaa
      181 tgctggcccg tttacgtccg atacaaaaag gatgctggca atgaatacag catttgcact
      241 tgttctgaca gtttttcttg tttccggaga gccagttgat attgcagtca gtgttcacag
      301 gacaatgcag gagtgtgtga ctgcagcaac cgaacagaaa attcccggta actgttaccc
      361 ggtcgataaa gttattcacc aggataataa cgaaatcccg gcaggtcttt aaaacagttc
      421 cgtaataaac atccgatttc attcttatat gccagcaatg gcagggattt gttcaccctt
      481 aaatctgtaa tgaggtaaaa caaaatgagt aaagtcttta tttgcgccgc cattccggac
      541 gaacaggcaa taaaggaaga aggtgcagtc gctgtagcca ctgccattga agccggtgat
      601 gaacgtcgcg cccgcgcaaa atttcactgg caattcctgg agcattatcc ggctgctcag
      661 gactgcgctt ataaatttct tgtttgcgag gataaacccg gtataccccg ccctgccctc
      721 gattcctggg atgctgaata tatgcaggaa aaccgctggg atgagggggc tgcttccttt
      781 gtcccggttg agactgaatc cgatccgatg aacgtcgctt ttgacaagct ggcccctgaa
      841 gtacagaacg ctgtcatggt taagttcgac acatgtgaaa acatcaccgt tgatatggtg
      901 attagcgcgc aggaattgtt gcaggaagac atggcaacat tcgacggaca tatcgttgaa
      961 gcgttgatga aaatgccaga agttaacgcc atgtatccgg agcttaagct gcatgccatc
     1021 gggtgggtta agcataaatg taagcctggt gccaaatggc ccgaaattca ggcagaaatg
     1081 cgcatctgga aaaaacgtcg cgaaggtgaa cgcaaggaaa ccggaaaata cacgtctgtt
     1141 gttgatctcg cccgcgccag agtccaccga cagcacactg aaaactcagc agaaaaaatc
     1201 ccccctgtca ctgcagtcat tcgtcgcgaa tataagcaga catggaaaac actggatgac
     1261 gaactggcct acgctctctg gcctggtgat gtggatgccg gaaacattga cggcagcatc
     1321 catcgctggg caaaaaatga agttatcgac aacgaccgcg aagactggaa gcgtatctcg
     1381 gcatcaatgc gcaaacagcc tgatgccctt cgctacgacc gccagactat ttttggcctt
     1441 gtccgtgaac gtccgatcga cattcacaaa gaccctgtgg cactgaacaa atacattact
     1501 gaatacctga ctacaaaggg cgtgtttgaa gatgaaggaa caaatcagag cgcaactgac
     1561 actctctcgt cgccagtacc agaaactgat gcagtggaaa cggcaattcc ggacaacgaa
     1621 aaaaccgaat gcaaagtgga agtcgaacca tctgtagagc gtgaggggcc gttctacttc
     1681 ctcttcaccg acaaggatgg cgaaaaatac ggtcgcgcaa acaaactttc tggtctggat
     1741 aaggcgctgg ctgccggggc tactgaaatc acaaaagaag aatattttgc ccgaaaaaat
     1801 ggcacataca caggcttacc gcaaaatgca aataccgcac aaaattctga acaaccagaa
     1861 ccggtaaaag ttaccgctga cgaagtaaag aaaattatgc aggcagccaa tatcagccag
     1921 cctgacgccg aggaactgct tgcagtatca cgtggtgaat ttgttgaagg gattagcgac
     1981 ccgaatgatc cgaaatgggt taaggggatc cagacccgcg attctgtgaa ccagaaccag
     2041 caagaaacgg aacagaacga ccagaaagcg gaacaaaaca gcccaaatac gcaacaaaac
     2101 gagccagaaa cgaaacaacc tgaaccagta gtgcaacagg aaccggaaaa gatctgcacc
     2161 gcctgcggtc agagcggtgg cggcaactgc cctgattgtg gtgcggtgat gggtgacgca
     2221 acataccagg aaatattcga tggagagaat cagcctgaag ttcaggaaaa tgatccggag
     2281 gaaatggaag gtactgcaca tcagcacaag gagaacactg gcggcaatca gcatcatgcc
     2341 agcgatagtg aaactggcga ggcgtcagat cccttaatta aggcgaacgg tcatcataat
     2401 ctcacatcca ccagcagagc ggggattcat ctgatgatcg atcttgaaac catgggaaaa
     2461 aatcccgatg ccccgattat ctcaataggt gcaatatttt tcgatccgca aaccggagat
     2521 atgggaccgg aatttagtaa gactatcgat ctggatactg ctggcggagt cattgatcgg
     2581 gacaccatga aatggtggct taaacaatca cgcgaagcgc aatctgccat tatgaccgat
     2641 gaaatcccgt tagatgatgc actgttacaa ttgcgggaat ttatcgacga aaactccggt
     2701 gaattttttg ttcatgtctg gggaaatgga gccaacttcg acaacacgat tttgcgccgt
     2761 tcatacgaac ggcaggggag cccctgcccg tggcgttact acaacgatcg cgatgtacgc
     2821 acaatcgttg agctggggaa agccatagac ttcgatgcca gaacggctat tccattcgaa
     2881 ggtgagcgcc ataatgcact tgatgacgcc cgttaccagg caaaatacgt ttcagctatc
     2941 tggcaaaaac tgatcccgag tcaggctgat tcttaatgtt caactgtcgc cggttgtgac
     3001 tggtattctg caaccggcgc tcgtctgatg taagagataa agaaatcgat gagcgaagta
     3061 atcatgattg tctctcccgg caaatgggta tccgaagagc agttaattgc gctgaaagga
     3121 ataaaaaaag gtacgttaaa aaaggcccgg gaaaaatcgt ttatggaagg aagggaatat
     3181 aagcatgtcg ctcatgacgg tatgccatgg gataacagtc catgctttta caacctggaa
     3241 gaaattgatc gctggattga gcgccaggca tcagcaagac caagacgtca tcttacttga
     3301 ctaaaagcca cactaactaa tgagagaagt tgaaatgaaa tatccgacag gcgtggaaaa
     3361 ccatggaggg aaattacgta tctggtttgt ttataaaggc gtaagagtca gggaaaacct
     3421 ggggttcctg acacagcaaa aaacaggcgc gttgcaggtg agctacgcgc ctctgtttgt
     3481 tacgcaataa aaactggtgt tttcgactat gcaaaacagt ttccctcctc acgcaatctg
     3541 gaaaaatttg gtgaggcccg acaagattta accataaaag aactggctga aaaatttctg
     3601 gcactgaaag aaactgaagt cgcaaaaaca ctcaacacat accgtgccgt catcaaaaat
     3661 atcctgagca taatcggtga aaaaaatctt gcctcatcga ttaataaaga aaaattactg
     3721 gaggttcgta aagagctact gactggatac cagatcccca aaagtaacta tattgttaca
     3781 caaccaggga gatcggctgt aactgtaaat aattacatga caaatcttaa cgccgtgttc
     3841 cagtttggtg ttgataacgg ttacctggca gataacccgt ttaaggggat ctcgccatta
     3901 aaggaatcaa gaaccattcc ggatcctctt tcgcgggagg aattcatccg tcttatcgat
     3961 gcgtgcagaa atcagcaagc caaaaattta tggtgtaata ccgtcctcag tggtgatttt
     4021 taatgtgacg aaattacgcc ccggacaggt aacaaaaact tcagccttta cgatcttcat
     4081 gttcgattcc ttgcatcgct tgtcgtgatg catgaaatct acgcaactga gctactacca
     4141 tataagtatg aagatcgaaa aaagccggag tgatcacaaa aaaaggcgta tatttgcgct
     4201 gtgaatggtt gacaaaagat gaaatagaat accttttgtc agctgacact tcctcgtatc
     4261 ttattgataa aatggattta tgttcctacg tgcgccccca gcccacaaca atgatcaaca
     4321 tgccgcaaag cgcaatcaac ggccccgtcc agtcataaag agtcagtttc acgccatcca
     4381 caacgcgcag ccacatcaac gccgtgcaga cataaacgcc accataagcc gcgtaaacac
     4441 gcccactcgc cgcaggatgc aacgttaaca accagacaaa cagcgccagt gaaatccccg
     4501 ccggaagcaa cagccagata ctggcgtttc gttttaacca caaccagggc agaaagcatc
     4561 caataatttc acacagcgca gtagcaaaaa atagtaacgt tgttttaatc atctttgtca
     4621 cttattgaca tcatgtatag ttatagggcg acataatatc atcaatataa acaccctcct
     4681 ggtacgtttt gcgttcgcag tggatggtgt agaatcatct ttaatcattc atacagggaa
     4741 tgaattatga aaatcactct cagcaaacga atcgacctgc tcgcttttct gctgccttgc
     4801 gcactggcat tgagcacaac tgttcatgcc gaaactaaca aactggtgat tgagtctggc
     4861 gacagtgcac aaagccgcca gcacgccgct atggaaaaag agcaatggaa tgacacgggc
     4921 aatctgcgcc agaaagtgaa taaacgcact gaaaaagagt gggataaagc cgacgccgct
     4981 tttgataacc gcgataaatg cgagcaaagc gccaacatca atgcctactg ggagccaaat
     5041 actttgcgct gcctggaccg tcgaactggc cgcgttatta ccccctaacc tgttattgat
     5101 ttaaggaatg taaggacacg ttatgccaag cgcccacagt gttaagctac gcccgctgga
     5161 gcgtgaagat ttacgctatg tacatcaact cgacaataac gccagtgtga tgcgttactg
     5221 gtttgaggaa ccctacgaag cctttgttga actctctgat ctgtatgata agcatattca
     5281 cgatcagagc gaacgacgct ttgtggtgga atgtgacggc gaaaaagccg gtctggtgga
     5341 gttggtggaa attaaccatg tacatcgccg cgcagaattt cagataatta tctccccgga
     5401 gtatcagggg aaaggtctgg caacccgtgc cgccaaatta gcaatggact atggcttcac
     5461 cgttctcaat ctctataagc tgtatctgat cgttgataaa gagaatgaaa aagcgattca
     5521 catttaccgc aagcttggct tttcggttga aggtgaattg atgcacgagt tctttattaa
     5581 tggtcaatat cgtaatgcca ttcgcatgtg tatattccag catcagtatc tggcagagca
     5641 caaaacaccg ggtcagactc tcctgaagcc gaccgcacaa tagcattaat aataatcgat
     5701 cgtatttttg atggtgtaaa cccgttcgac ggcgggtttt actccttcat caacaatgat
     5761 cagctgacag cccaccggat tggcgtgact gtcatattca cagctctgtt ttacattacc
     5821 aactcgttga ttattcagta aagtaaccgc tgtgtaatct aattttttga tcgggtccgt
     5881 tgatggcgtg gcgctgacag ataatgtttt gtcgttactt ttcgtggttt tacccagtgg
     5941 ataaccctga tcatcatagc gatattccat ctgcatttgt ttgctgctgg ctttaatcac
     6001 gaagccatta tcatcggttt cccaactcac cccggcagaa ggtaattctg ctagctggca
     6061 ttttccctgt aaacgtactc tcttctccag cgtctcggca tcacggtaat aattggcgtc
     6121 cagtaccaga gcgaccacgg tattattttc cagatccagt aattcgagtg aatcaaaaca
     6181 gccttcttcc gacaaagtcc ccgaaacacg tttcgtcact tcaccttgct catccattaa
     6241 tgtctgagtg aaatctttta ccggaccacg cagcggatca aaatcgaatt cattagagaa
     6301 actggccatc tcaggagtaa atgaaagtgt tacctctgtg cggtcacatc ctgtgagtaa
     6361 tatcgcgagt aagcatggca gtaatttgta tttcacatca gtcaccagag agtagagatg
     6421 attctcaatc atagtagcaa atacagtact ttacacgtga aatgctatgc ttaaagaagt
     6481 tatcttcgcg ttaggagctt atgatgaaac tctcaacctg ttatgccgcg cttctgctcg
     6541 ccctcgcctc acccgcggta ctcgccgcgc cgggatcctg tgagcgcata caaagcgata
     6601 tatcacagcg cattatcaat aatggcgtac ccgaaagcag cttcacgtta agtattgtac
     6661 ccaatgacca agttgatcag cctgattccc aggtcgtcgg gcattgcgct aatgatacgc
     6721 ataaaattct ctacacccgc accaccagcg gtaacgtctc tgctcccgcg cagtctaccc
     6781 aggatggcgc gcctgccgaa ccgcagtaat acattatcga ctacacgccg gatacgacac
     6841 atccggcgat ttggacatac aacataatcc caccatatta ctcataccct tctattgata
     6901 tggattaata attattaacc caaaatgggt agactccctc tattgttagc gcgctaaata
     6961 ttcaatatat aaacttttat ataacgataa agaacaggga gtgagttatg tccaaaaatg
     7021 aacgaatggt gggcatcagc cgcagaacac tcgttaaatc taccgcgata ggttctctgg
     7081 cgctggctgc aggcggtttt tctttgccgt tcaccctgcg cagtgcagcg gcagcagtac
     7141 aacaggccag cgaacaagtg gtctggggtg cctgttccgt caactgtggt agccgctgtg
     7201 cacttcgtct acatgttaaa gataatgaag tggcctgggt ggaaactgac aacaccggca
     7261 gcgatgagta tggcaaccat caggtacgcg cctgtttgcg cggtcgctcc atccgccggc
     7321 gtattaatca tcccgatcgc ttgaattacc caatgaaacg cgtgggtaaa cgcggtgaag
     7381 gcaaattcga acggattagc tgggatgaag ccctggatac tatcgccagt agcctgaaga
     7441 aaaccgtcga acaatatggc aatgaggctg tatatattca gtactcttcg gggatcgttg
     7501 gcggcaatat aacccgctct tcgccatcag cctcggcggt caaacgcctg atgaactgct
     7561 acggcggttc actcaaccag tatggctcct acagcactgc gcaaatttcc tgtgccatgc
     7621 cctacaccta cggcagtaat gatggcaaca gcaccacgga tattgaaaac agcaagctgg
     7681 tcgtgatgtt tggtaacaac ccggcagaaa cccgcatgag cggcggtggc atcacttatc
     7741 ttcttgaaaa atcccgcgaa aaatcgaacg caaaaatgat tgttatcgat ccgcgatata
     7801 ccgatacggc tgctggtcgt gaagatgaat ggcttcctat tcgcccgggc accgatgccg
     7861 cgctggttgc gggtattgcc tgggtattga ttaacgaaaa tctcgttgat caaccttttc
     7921 tcgataaata ctgcgtcggc tatgacgaaa aaaccttacc cgcagatgca cccaaaaatg
     7981 gtcactataa agcctatatt cttggtgaag gtgacgataa aacagcgaaa acgccgcagt
     8041 gggcttcgca aattaccggt atcccggtgg accgtatcat caaactggcg cgtgaaattg
     8101 gcacagcaaa acccgcttat atctgccagg gctgggggcc gcaacgccag gcaaacggcg
     8161 agctgaccgc acgcgctatt gctatgttgc ctattctgac gggcaatgtc ggcatcagcg
     8221 gcggaaatag tggcgcgcgt gaatcgacct ataccattac catagagcgc ctgccggtgc
     8281 tggataatcc ggtcaaaacg tcaatttcct gcttcagctg gacagatgct atcgatcatg
     8341 gcccacaaat gacggcaatc cgcgacggtg tccggggcaa agataaactg gatgtgccca
     8401 ttaagttcat ctggaactac gcgggaaata ccctggttaa tcagcattct gacatcaaca
     8461 aaacgcatga aattctgcag gacgaatcga aatgcgaaat gattgtggtc atcgaaaact
     8521 ttatgacctc atcggcaaaa tatgccgaca ttctgctgcc agacctgatg accgttgagc
     8581 aggaagatat tattcctaac gactacgccg gtaacatggg atatctcatt ttcctccagc
     8641 ctgtcaccag cgaaaaattc gaacgcaaac cgatttactg gatcctgagt gaagtcgcga
     8701 aacgtcttgg acctgacgtc tatcaaaagt tcactgaagg tcgcacgcag gaacaatggt
     8761 tacaacatct gtacgccaaa atgcttgcca aagatccggc gttaccgtct tacgacgaac
     8821 tgaaaaaaat gggtatctat aagcgtaaag atcccaatgg tcattttgtc gcctacaaag
     8881 catttcgtga cgaccccgag gcaaatccac ttaaaacacc ttccggtaag attgaaattt
     8941 attccagcaa gctggcggaa attgcccgta cctgggaact ggaaaaagat gaagtgataa
     9001 gcccactgcc agtttatgcc tcaacctttg aaggctggga ctcccctgaa cgtagtacct
     9061 ttccactgca actgtttggt ttccattaca aatcccgtac tcactcgacc tacggcaaca
     9121 ttgatgtcct gaaggctgcc tgccgtcagg aggtgtggat caaccctata gacgcacaga
     9181 aacgtgggat tgccaacggc gatatggtgc gggtgtataa ccatcgtggc gaagttcggc
     9241 taccagccaa agtgacacca cgcattctcc ctggggttag cgctatgggc cagggagcct
     9301 ggcacgaggc caatatgtct ggcgataaaa tcgaccatgg cggatgtgtg aatacgctaa
     9361 ccactctgcg cccttcacca ctggcgaagg gaaacccgca gcacacgaat ctggtcgaga
     9421 tcgaaaaaat ataacccact acaaccataa attctggcat gacattttgt ttgaaaagca
     9481 ataagtgagt aatgatgaaa atccatacca cagaggcgct catgaaggct gaaatcagcc
     9541 gtagaagtct gatgaaaacc tccgcacttg gcagtcttgc gctggcaagc agtgctttca
     9601 ctctgccatt ttcccaaatg gtccgagcgg cagaggcacc ggtagaagag aaagcggtct
     9661 ggagttcctg caccgttaac tgcgggagcc gctgtctgtt acgtttgcat gtgaaagatg
     9721 acaccgtgta ctgggtggag tctgatacga caggtgacga cgtctacggt aatcatcagg
     9781 ttcgtgcgtg tttacgcggg cgctctatcc gccgacggat gaatcatcct gataggttga
     9841 aatatcccat gaagcgcgtc ggcaagcgcg gtgaaggtaa atttgaacgg ataagttggg
     9901 acgaagccct ggataccatc agtgataatc ttcggcggat cctgaaagat tacggcaatg
     9961 aggctgtaca tgtcctgtac ggaacaggcg tagatggcgg aaacatcacc aactcaaacg
    10021 tcccgtaccg tctgatgaac tcttgcggtg gttttctcag tcgctatggc agctacagta
    10081 ccgcacagat cagtgccgca atgagttata tgttcggtgc caatgatggc aacagcccgg
    10141 atgatatcgc caatacgaaa ctggtcgtta tgttcggaaa taacccggca gaaacgcgga
    10201 tgagcggtgg tggtgtcact tactacgtcg agcaagcccg cgaacgttca aacgcacgca
    10261 tgatcgtcat cgatccacgt tataacgaca cagctgccgg gcgtgaagat gaatggctgc
    10321 ccattcgccc tggcaccgat ggcgcactgg cctgtgcgat tgcctgggta ctgattagtg
    10381 aaaacatggt cgatcagcca tttctcgaca aatattgtgt tggttacgat gaaaaaacgc
    10441 tgcccgccaa cgcaccacgt aacgcgcatt ataaagccta tattctgggc gaagggcctg
    10501 acggcatagc taaaacgccg gaatgggcag caaaaatcac cagcatcccg gcagaaaaaa
    10561 ttatccagtt ggcacgagag atcggttcag caaaacctgc ttatatttgt cagggttggg
    10621 ggccacaacg acattccaac ggcgagcaaa catcccgcgc tattgccatg ctttccgttc
    10681 tcaccggtaa cgtcggcata aacggcggca actcaggtgt acgcgaaggt agctgggatc
    10741 tgggggtaga atggttcccg atgctcgaga atcctgttaa aacgcagatt tccgtcttta
    10801 catggacaga tgccatcgac catggtacgg aaatgaccgc gacccgtgat ggtgttcgtg
    10861 gaaaagaaaa actggatgtc cccatcaagt ttttatggtg ctacgccagt aacacattga
    10921 tcaatcaaca tggcgacatc aatcacacca atgaggtgct tcaggatgac agcaagtgcg
    10981 agatgattgt tggcattgac cacttcatga cggcctcggc taagtattgc gatatcctgt
    11041 tgcccgacct gatgccaaca gagcaagaag accttatctc tcatgaatct gcagggaata
    11101 tgggctatgt gatcctcgcc caacccgcaa cctcagcaaa atttgaacgc aaacccatct
    11161 actggatgct gagtgaagtc gccaaacgct taggaccgga cgtttatcaa acctttactg
    11221 aaggtcgcag tcagcatgaa tggatcaaat atctccatgc gaaaacgaag gaacgtaacc
    11281 ctgagatgcc cgactacgag gagatgaaaa cgaccgggat ctttaagaaa aaatgcccgg
    11341 aagaacacta cgtcgctttc cgcgcattcc gtgaagatcc acaggcaaac ccgttgaaaa
    11401 caccttcggg gaaaatcgaa atttattctg aacgactggc gaagattgca gatacctggg
    11461 aattgaaaaa agatgaaatt attcatcccc ttcctgcgta taccccaggt tttgatggct
    11521 gggacgatcc cctgcggaaa acctatccac tgcagttaac gggcttccac tacaaagcgc
    11581 gtacccactc cagctacggc aatattgatg tgttacagca ggcctgccca caagaggtgt
    11641 ggatcaaccc cattgatgct caggctcgcg gtatccgtca tggcgatacc gtgcgggtat
    11701 ttaacaataa tggagaaatg ctgattgccg caaaagtgac tccgcgtatt ctgcctggcg
    11761 ttaccgccat cgggcaaggt gcgtggctta aagcggatat gtttggtgac cgggtcgatc
    11821 acggcggcag tatcaatatt ctgacctctc accgcccttc accgctggca aagggaaacc
    11881 cgtcgcacag caatcttgtc cagatcgaaa aggtttaagg agtagcccat gaccacacaa
    11941 tatggatttt ttatcgattc cagccgctgt actggctgta aaacctgcga actggcgtgc
    12001 aaagatttta aagatcttgg cccggaagtc agtttccgcc gtatttatga atatgctggc
    12061 ggcgactggc aggaagataa tggcgtctgg caccagaacg tgttcgctta ttatctctcc
    12121 atctcctgta accattgcga tgaccccgca tgtacaaaag tctgccccag cggcgcaatg
    12181 cataagcgtg aagatggctt tgtggtggtt gatgaagatg tttgtatcgg ctgccgctac
    12241 tgccacatgg cttgtccgta cggcgcgcca cagtacaatg ctgaaaaagg gcacatgacg
    12301 aagtgcgatg gttgttattc gcgcgtcgcc gaggggaaac aacccatatg tgtcgaatcc
    12361 tgcccgctgc gtgcacttga gttcggtcca attgaagagc tgcgccagaa gcacggcact
    12421 cttgctgccg tcgcgccact gccgcgcgcg catttcacaa aacccaatat cgttatcaaa
    12481 cctaacgcca acagccgccc gaccggcgat accactggtt atctggctaa tccggaggaa
    12541 gtgtaaaatg ggaaatggat ggcatgaatg gccactggtt atctttaccg tattgggtca
    12601 atgcgtagta ggcgcgctga ttgttagcgg aattggctgg ttcgccgcca aaactgacgc
    12661 agaccgtcag cacattgttc gtggcatgtt tttcctctgg ctgttgatgg gcatcgggtt
    12721 tattgcctct gtcatgcacc tgggatcgcc actgcgggcg tttaactcgc tgaatcgaat
    12781 cggagcttct ggtctgagta acgagattgc tgccggttcc atattttttg ccgttagcgg
    12841 actatggtgg ctggtggcgg tcatcggtaa aatgccgcaa gcgttgggga aactctggtt
    12901 acttgtcagc atggcgctcg gcgtcatttt cgtctggatg atgacctgcg tgtatcaaat
    12961 cgacactgtg ccaacctggc ataacggtta cactacgctg gctttcttcg tgacggtatt
    13021 gttgagtggt ccaatacttg ctgccgcaat tctgcgggca gcccgcgtta catttaatac
    13081 cacaccattt gccatcatta gcgttctggc gctgatcgct tgcgccgggg tgattgtgct
    13141 gcaaggtttg tctctgtcgt ctattcactc atccgtgcaa caagccagcg cactggtacc
    13201 agattacgcc tccttacagg tctggcgtgt ggtattgtta tgcgcaggtc taggatgctg
    13261 gctttgtccg ctcatacgtc gccgtgaacc tcacgttgcc ggacttattc tggggttaat
    13321 cctcattctt ggcggtgaga tgatcgggcg tgtgctcttt tatggcctgc acatgacggt
    13381 aggaatggct attgcaggtt aacaaattgg tgcggggcaa cccgcacatt tcaggatgtt
    13441 aggaatgacc catttttcac agcaagataa tttttctgtc gcggcgcgcg tgttgggcgc
    13501 gctgttttat tacgctccag agagtgctga agccgcacct ctggttgcgg tactcaccag
    13561 tgacggctgg gaaactcagt ggcctctacc agaagcgtca ttagcgccgc tggtgacggc
    13621 ttttcagaca cagtgtgaag agactcacgc ccaggcctgg cagcgtttgt ttgtcggccc
    13681 gtgggcactg ccgtctccgc cgtgggggtc ggtctggctg gatcgcgaat ctgtgctgtt
    13741 tggcgattca acattggcac ttcgtcagtg gatgcgcgag aaaggcattc agtttgaaat
    13801 gaagcaaaac gaaccggaag atcattttgg ttcactgctg ttgatggctg cgtggttggc
    13861 ggagaatggt cgccagacag aatgcgaaga attgctggca tggcaccttt ttccctggtc
    13921 aacacgtttt cttgacgttt ttatcgaaaa agcagaacac cctttctacc gtgcactggg
    13981 tgaactggct cgcttaacgc tggcgcaatg gcaatcacaa ctgttaattc ctgtcgcggt
    14041 taaaccactc tttcgatagg atcaccgtaa tattgccgga tggtgacgat tacgcgcgat
    14101 tcggcaacaa tacgttacag tgtcacgttt tttgtctctt aaagcacgca ctgcttttgc
    14161 agctggcctc ttttgccgca aaatagtcgc ccgtgtttca ttgcccattt ctgctcatgc
    14221 atcatctaca cacctatccg gatctgcgca ctatgttccg ccgtctgctt atcgcaacag
    14281 tcgtcggtat tctcgcggcc tttgccgttg ccgggtttcg tcatgcgatg ctgctactgg
    14341 agtggttgtt cctcaataat gactccggca gtctggtcaa tgcagcgaca aacctttccc
    14401 cctggcgacg gctgctaact ccggcgctcg gcggactggc ggcgggtttg ttgttgatgg
    14461 gctggcagaa atttacccag caacgccctc atgcgccgac cgattacatg gaagcgttgc
    14521 aaaccgatgg tcagtttgat tacgcagcaa gcctggttaa atcgcttgcc tctctgctgg
    14581 tagtaaccag cggcagtgca attggtcgcg aaggtgcgat gattctttta gctgcccttg
    14641 ccgcctcctg ttttgcccaa cgttttacgc cacgccagga gtggaaatta tggatcgcct
    14701 gtggggccgc ggcgggaatg gctgcggcct atcgtgcccc gcttgctggc agtttattta
    14761 tagccgaagt gctgtttggc actatgatgt tggcctctct cggcccggtg attatttccg
    14821 ccgtcgtggc attgctggtt agcaatctga ttaatcatag cgacgcgtta ctctacagcg
    14881 tacaactctc agtgacggtt caggctcgtg actatgcgct gattatcagt acaggtgtgc
    14941 tggcaggtct gtgcggacca ctgttgttaa cgttaatgaa cgcctgtcat cgtggatttg
    15001 tgagtctcaa acttgcgccg ccctggcaac tggcactagg cgggttgatc gtgggtctgc
    15061 tttccctgtt cacacctgca gtgtggggca acggctatag caccgtacaa tcctttttaa
    15121 ccgccccgcc actgttaatg atcattgccg ggatcttcct ttgtaaactg tgtgccgtgc
    15181 tggcgagtag cggttccggc gcacccggtg gtgtctttac accgacgcta tttatcggtc
    15241 ttgccattgg catgttgtat ggtcgtagtc tgggattatg gttccctgat ggcgaagaaa
    15301 ttacgctttt actcggattg accgggatgg cgacactgtt ggcggcaacc acgcacgcgc
    15361 cgattatgtc gacgttgatg atatgtgaaa tgaccgggga gtatcagcta ctccccggtt
    15421 tattgattgc ctgcgtaatt gcgtcggtaa tctcgcggac gttacaccgt gactctatct
    15481 accgccagca cactgcgcag catagctaag cggatgtatt gccccagttc acgctgttcg
    15541 gcgcgcggca gataaggcag ttcaccaatg agcggtgccg gaagtttttt accgagcaca
    15601 tcaatgattt ccgcataatg cgccagtcct gggttgattc ggttagccac ccagccaatg
    15661 agcggcagcc cgtcgttggc aatcgcctga gctgttagca gtgcatggtt aatgcaacct
    15721 tcctgaatac cgacaaccat caacaccggc agttgttcct gcaccaccca ttcagagagt
    15781 ggacgcaaat cattcatcag actacgccag ccgccagtcc cttctaccac gacatgatcg
    15841 actttttcgg tcaggtttgc caggccgttt gaaatgaggg tgtaattgat tgggcaactg
    15901 tgcgccacgc tactttcttc ttcgcttaac gcgataggat taactgcttc ataaggcagt
    15961 tcgatggttg aaacactctg caacaccagg gcatctttat tacgcagccc ttcgggtgtc
    16021 tctttgctcc ccttcgctac gggtttatat cccgcaaccg tttttccctg ggaggctaac
    16081 gcttgtagca atgcgcggga aaccaccgtt ttccctacag aagtgtctgt accggtaata
    16141 aagaaacgct tcagcatcac taactccacc gttatgcttc acaaatataa accaggaaaa
    16201 taattaacct tgaaagtcta agttatgctt tcctggccca aattgagata gcgcaaattt
    16261 tggtagaaca gttaaaaaat gttaaccctg caacagacga atcaacaaag aaccgttata
    16321 catcgcgtct tttaccagcg cagcccctgc catcgtgccc tggttagaaa actgagtact
    16381 ctcaacgcta atgtgctgac tatacgcagg aagggcctgc tgacggatgc tatctgagat
    16441 gaccgggaag aggatatctg ccgctttact taacggtgag ccaatcagta ttttttgtgg
    16501 gttaaataaa ttcaccatga tggcaagaat gcgccctaca tgcgcgccca ccccggtaat
    16561 gatgtctttt gccagcagat cgccgcgcaa tgccgcctga cacaatgagt ccacggttaa
    16621 cggttgccca tgtaacatcg agctcatgga ttgattgaga cgcagctgtg ccagctcaag
    16681 aatactgtcc acactggcga tggtttcgag gcagccgtgg ttcccgcaat aacagcgttt
    16741 accatatggg tctacctgtg tgtgacctat ttccacgagg ctactgctgc ctgcgtgtag
    16801 cagatgacca tcggtaatga cgcccgcccc cacgttgtga tcgataacca cctgaatcac
    16861 atcgcgcgcc ccgcgtgagg caccaaacaa ggcctctgcc atcgtccatg cgctgatatc
    16921 atgctgaata taaaccggaa cgccggtatg ctgctccaga gcctcgccga gcggcatctc
    16981 ttttacatcc tcgtagaacg gcatgcgatg tacaatgcca ttttccgtat caataattcc
    17041 cggcagggtt atggcaatcg aagttagacg ctcaagtttt ttctggtggc ggataaaaaa
    17101 ctgatcgata tgggaaatga tacgatccag caatggcgag tcatctttta acgccagttc
    17161 ctgcgactct tccaccacca gtttgctgct cagatcgcgc agagcaagga aaatctcccc
    17221 gcgactaatg cgcagagaaa gatagtgcca ggcttcagtt tcaaccacca gccccaccgc
    17281 cggacggcca cggttccccg cttctttgat ttccagctct tgcaccaggt gtgcttcgag
    17341 catctcacgg acaattttag tgatactggc aggagccagc tgcgccagac gggaaagatc
    17401 gatacgcgag actggaccaa gctgatcaat caggcgataa accgcgcccg cgttggtctg
    17461 ctttatttga tcaatgtgcc caggctggtt ttcagcaacc accgcatact ccctatattt
    17521 tcgcgctccg aaataatctg taggctatgg tgaagcactt caatacgtgt cgtcaaattt
    17581 ttacttaggc atgtgattaa cagcacattt ttcgggcttt ttcgctgaaa tttccctcac
    17641 ctgagagcat tcagcagatg aatacgaaag ttacgcgcag ctggactttg ttcatgatgt
    17701 ttcggccaga ccaaccacat ttcagaaacc gcatcctctt cagcaatcgg cacccagcgc
    17761 atttcgttga gctgaacacg tttaaatgac gcaggcaaaa ttgaaacacc cagaccggcg
    17821 gaaaccagac cgatgatggt cattgcctcg cccacctcct gagtgatgac gggcgtcaaa
    17881 tggtaacgtc gcatcagccc gagaatatcg tcatacagcc ctgtcccgac gtgcggatca
    17941 aaaaagacaa agggttcttt cgccagttca gccagcgtta cattcgggtt atttgccagg
    18001 ggatgatcgt gcgggatcat cgccataagc ggttcatgga cgatgactgc gtgttcaagc
    18061 gtctccggta acgctgtgtt acgcagcaat cccatatcca gcgttccttc aatgagcgga
    18121 gcgatttgct cgcgagtgtt catttcgcgg gtttgtaaat ggacatcggg ataatctcgg
    18181 cgaaacagcg ataacgtatc ggacaccgcc cgaataaaag gagccgacga agtaaaacca
    18241 atgcgcaact cccccgcttc accctgatgc agtctttcag cgcgagcggc agcgtcatcc
    18301 accatagaca ggatttgccg actatctgca agaaactgtt ttcctgctgc cgtcagcaat
    18361 acactgcgat tggttcgtgc cagcagtcgg gcaccaattt gttgctccag cgcctgaatc
    18421 tgctgactta gcggcggttg cgaaatattc aggcgggcag cggcgcgccc gaaatgcagc
    18481 tcttccgcaa cagcaacaaa gtaacgcaga tgacgaagtt caatattcat atttaaaaca
    18541 tcttatttga gattattaat atattagaca gaacaattcg attttcctaa cctatgtata
    18601 agcctgatcc acaggcatat ttagcaagga tttcaagtga gccgtactac aactgttgat
    18661 ggcgctccgg caagcgacac tgacaagcaa agcatttctc agccaaatca atttattaaa
    18721 cgcggtacgc cgcaatttat gcgcgtcacc ctggcgctgt tctctgccgg actggcaaca
    18781 tttgcacttc tctattgtgt gcaacctatc cttccggtgc tttcgcagga gtttggctta
    18841 acgcccgcga acagtagtat ttcactgtcc atttccacgg cgatgttggc tattggtttg
    18901 ctgtttactg gccccctatc cgatgccatt ggtcgcaaac cagtgatggt aacggcgcta
    18961 ctattggcct ccatttgtac gttactttcg acaatgatga ccagctggca cggcattttg
    19021 attatgcgcg ccttgattgg gctttcgtta agtggcgtgg cagctgttgg catgacttat
    19081 cttagcgagg aaatccaccc cagtttcgtg gccttttcga tggggttgta tatcagcggc
    19141 aactcaattg gcggcatgag cggacgctta attagtggtg tcttcacgga ctttttcaac
    19201 tggcgaattg ctctggcggc tatcggttgt ttcgcgctag cctcggcgtt gatgttctgg
    19261 aaaatcctcc cggaatcacg ccattttcgc ccgacttcgc tgcgacctaa gtcgttgttt
    19321 atcaactttc gtctgcactg gcgtgaccgg ggattaccgt tattgttcgc agaaggcttt
    19381 ttgctgatgg ggtcgttcgt cacgctgttt aattacatcg gctatcggtt gatgctctcc
    19441 ccctggcatg tcagtcaggc cgtggttggc ttattatcgc tggcttattt gaccggtaca
    19501 tggagctcac ccaaagccgg aaccatgacc acccgctatg ggcgtggtcc agtgatgttg
    19561 ttttcgacgg gggttatgct gtttggttta ctgatgacct tattcagctc gctgtggctg
    19621 atctttgccg gaatgttact cttctcagca ggattcttcg cagcccactc agttgccagc
    19681 agctggatcg gcccccgcgc aaaacgcgct aaaggccagg cctcctcgct gtatctgttc
    19741 agttactatc tggggtcgag tattgccggg acgctgggtg gtgttttctg gcataactat
    19801 ggctggaacg gcgtcggcgc atttattgct ctgatgctgg tcattgctct gctggtcggg
    19861 acgcgtttgc atcgtcgtct gcacgcctga aaaataagtc cggactgcgg taaatacccg
    19921 tccggactta ttgcctgctc aaaccaacgt taatagccat cctaaaatag acgaagcgcc
    19981 agccaattcc cgcagcgcgt ctagcgtcat caggattata agtacccaaa taaacggatt
    20041 cattttgctg tgtgtcattt attactgatg cgcagttatt ctaccgcttt gtaagtagta
    20101 aaatagttaa cccgatcaag actactatta ttggtagcta aattttcctt aagtcacaat
    20161 acgttattat caacgctgta atttattcag cgtttgtaca tatcgttaca cgctgaaacc
    20221 aaccactcac ggaagtctgc cattcccagg gatatagttc tttcaacggc cccgcagtgg
    20281 ggttaaatga aaaaacaaat tgagggtatg acaatgaaaa aagtattagc tctggttgtt
    20341 gccgctgcta tgggtctgtc ttctgccgcc tttgctgcag agactgcgac cacacctgct
    20401 ccgactgcga cgaccaccaa agcagcgccg gcgaaaacta cacatcataa aaaacagcat
    20461 aaagcagcac ctgcccagaa agcgcaggcg gctaaaaagc atcataaaaa tacgaaagct
    20521 gaacagaaag ccccagaaca aaaagcgcag gcagcgaaga aacacgccaa gaaacacagc
    20581 catcagcaac cggcaaaacc tgctgcacaa cccgcagcgt aagttttcaa cagtaatgct
    20641 ggcgcgcccc ctcgcgcctg aaaattacgg tgctaagcgg gtaacgttta gcaccgcctt
    20701 tagcggaggg tagtatggtg ggccgttatc gctttgagtt cattcttatc atccttattt
    20761 tatgcgcact gattgccgcc cgtttttatc tttcctgatc gtagttatct gattttactc
    20821 ccactttcat cccgtcccgt ctatagtatt tatgagggtt tgcttttaat aatcataatt
    20881 acccaccaga gtgtgatatg cgtacaacca ttgctgtagt gttgggtgca attagtttga
    20941 cgtctgcttt tgtgtttgca gataaaccag acgttgccaa atcggcaaac gatgaggtca
    21001 gcaccctgtt ttttggtcat gatgatcgtg tgccagtgaa tgacacgacc caatcaccgt
    21061 gggatgcggt tgggcaactg gaaacggcca gcggcaattt atgtacggcg acgctgattg
    21121 cacccaatct ggcattaacg gcaggacact gtttattgac gccaccaaag ggtaaagccg
    21181 ataaagcagt ggcgctgcgt tttgtgtcaa ataaaggtct ttggcgctat gacatccacg
    21241 acatagaagg ccgcgttgat ccgacactgg gaaagcggtt aaaagcagat ggggatggtt
    21301 ggattgtacc tcccgcagcc gcgccgtggg acttcggatt gattgtgcta cgtaatcccc
    21361 cttctggcat tacgccgttg ccgttatttg agggagataa agccgcgctt accgccgcat
    21421 taaaagcggc aggtcgtaaa gtgactcagg caggataccc tgaagatcat ctcgatacgt
    21481 tgtacagtca tcaaaactgt gaagtgactg gctgggcgca aacgtcggtg atgtcacatc
    21541 agtgcgatac cttgccgggt gacagcggtt cgcctctgat gttgcatacc gatgacggct
    21601 ggcaattaat tggggtgcaa agttcggctc ctgccgcgaa agatcgctgg cgcgccgata
    21661 accgggccat ttctgttacc ggttttcgcg acaagctgga tcaactgtcg caaaaataat
    21721 gttcaaacgc tgcccgacag cgcgggcagc gtcttcatca ggcaagtttc accatgatca
    21781 ttccagccaa cagcaagacc aggccaatcc agcctttacg atttaaccgt tgaccaaaca
    21841 agatccaacc tgcggctaac gtggcggcaa taccaaaccc gccccacaat gcataagcga
    21901 cagacaagtc gatcccttta acggcctgag aaagcgcact aaaggcagcc agcaccgccg
    21961 ccagggagag caagccaaat attttgcgac gaaagccgtc agaaaatttc aaaaagacgt
    22021 tagcaacgat ttccagcacg attgccaatg ccagccaggc ggcgtgaacc cattcaaact
    22081 gcgccatggt tcacctccag ttcaggttta cgtgctttac gggtacctga tttgatcaac
    22141 actatcccgg cgaccagggt ggttaacccg gcaattttca tcagcgataa actttcgtcg
    22201 aataacaaaa cgctaaacaa ggtaataaat aaaataccga taccttccca cagcgcataa
    22261 gccacaccta aggctatttt tttaacagcg aaagagagaa atatatacga cagagaaatc
    22321 atcaccagca ttaaaataaa gccgccattt ccctcactga cgctcgccca tttcatagac
    22381 agcgtaccgg taatttctgt agcaatagcc agacctaata aaatccaata aatatacatt
    22441 gtccttctcc tgcaagagaa ttattttaat tttcgcttaa ttcagcgaaa ccaaagtaaa
    22501 gtggtcaagc cccgcgtata gggcaagctt aggcagaaga aaggactaaa gcgcgttgcg
    22561 ccagtgctgc tcacctacga gcaagatagt ggatgaggta cgaagatggg taaatgtaga
    22621 aaataacgtc ctgaacaaat tgtccataat attacaatta tccgcagtgt tgcttctcgt
    22681 catcgcggat gataattgtc ctcggtagtt gaacacgcct gatttgtatc atagcttaag
    22741 aattaactca aaatattttc acttctttac ctgagcggtt tgattttcgt tatgatgacg
    22801 gagcgaaaaa gacattatta ttagcaaagg aagaaaaaac ggggacaagc atggcaaagc
    22861 cgctcatcac gctcaatggc ctaaaaatcg tcattatgtt gggaatgctg gtcattattc
    22921 tctgcggtat ccgttttgcc gccgagatca tcgtgccgtt tattctcgca ttatttattg
    22981 ctgttattct taacccgctg gtgcaacaca tggtccgctg gcgtgtgccg cgtgtactgg
    23041 cagtgtcgat tttgatgacc atcatcgtga tggctatggt gttgctgtta gcttatctgg
    23101 gttccacgct caacgagttg acgcggacgt taccgcaata tcgcaactct attatgacgc
    23161 cgctgcaagc tcttgagccg ttgttgcaac gcgtagggat tgacgtctca gttgaccaac
    23221 tggcgcatta cattgatccg aacgcggcga tgacgttgct caccaactta ttgacgcagt
    23281 tatctaatgc catgtcatca atatttttat tgctgctgac ggtgctgttt atgctgctcg
    23341 aagtgccaca attgcccgga aaatttaagc aaatgatggc gcgtccggtt gaagggatgg
    23401 cggcgattca gcgtgcgatt gacagtgtgt ctcattatct ggtgctgaaa acagccatca
    23461 gcatcatcac cggcctggtc gcatgggcga tgctcgccgc cctcgatgtt cgcttcgctt
    23521 ttgtctgggg attgctggcc tttgcgctta attacatccc taatattggt tcagttctcg
    23581 cggcaatccc ccctattgct caggtactgg tgtttaatgg cttctacgaa gcgttgttgg
    23641 tgctggcggg atatctgctg attaatctgg tcttcggcaa tattctggag ccacgcatca
    23701 tggggcgtgg gctgggactt tccacattgg tggtattttt gtcgttgatt ttttggggat
    23761 ggttgttagg accggtgggt atgctgcttt ccgtgccgtt aacaattatt gtcaaaattg
    23821 cgcttgaaca aacagcggga ggtcaaagca tcgccgttct gttaagcgat ctcaataaag
    23881 agtgacggcc tccgcagagg ccgtcagggt tacagagctt tcaggattgc atccacgctg
    23941 gctttggcgt caccaaacag catgtgggtg ttttccttga agaacagcgg gttttgcaca
    24001 ccagcatagc cagtgttcat cgaacgttta aagacaatca cgttctgcgc tttccacact
    24061 tccagcacag gcataccagc aatcggactc ttcggatcat cctgcgccgc cgggttaacc
    24121 gtatcgttag caccaatcac cagtacggta tcggtatcag caaagtcatc gttgatctcg
    24181 tccatttcca gcacgatgtc atacggtact tttgcttcag ccagcaatac gttcatatgt
    24241 ccaggcaaac gccccgcaac cgggtggata ccgaaacgca cgttgatacc acgagcgcgc
    24301 agtttctcgg taatttcagc gacaggatat tgcgcctgcg cgactgccat gccgtacccc
    24361 ggagtaatga tcactgaatg ggagtttttc aacagttccg ctgtctcttc tgcggtgatt
    24421 tcgcggtgct cacccacttc ctgatcatcg ccagtagaag agccgtcggt gccgaaacca
    24481 cccgcaataa cgctgataaa ggaacggttc atcgccttac acataatgta agaaaggata
    24541 gcccccgagg aaccgaccag cgcaccggtc acaatcagca ggtcgttgct gagcataaag
    24601 cccgcagccg cagccgccca gccggagtac gagttcagca tcgacaccac caccggcata
    24661 tctgcaccac cgatggaggc gactaaatgc cagccaaata ccagcgcaat ggcggtcatt
    24721 attagcaatg ccagcacttg caggccgacg ctatccgtgc gaacaaatac aatcagcagc
    24781 aggaaggaaa cgaccagagc tgccaggttc attttgtgac ggtttggcag catcaacggt
    24841 ttagacgaaa ttttgccaca cagtttgccg aacgccacca ccgaacccgt gaacgttacc
    24901 gccccgatga agataccgag aaacacttcc gtcaggtgaa tattgaccag aatcggtgcc
    24961 attcccgcgt catgatgcag atagctgtta aagccaacca gcactgccgc cagacccacg
    25021 aagctatgca ggatcgccac cagttctggc atttcggtca tttcaacttt cttcgccaga
    25081 cggataccaa ttgccccacc aatgaccatc gccagcaaga tccagccaac gttacccgta
    25141 tccggtccaa aaatggtggc gattagcgca atcgccatcc cggcgatacc gaagttgtta
    25201 ccctggcgag acgtttcatg tttcgaaagc ccggccagac tgaagataaa caggatcgcg
    25261 gcaacaatgt atgcagctgt aactaatcct ccagacatat gttacccctt aatttttgcg
    25321 gaacattttc agcatgcgct gagtcacggt gaagccaccg aaaatattaa tgctggctat
    25381 aagcaccgcg ataaaactaa ggaagctaac ccagccgccc tggccaatct gcaacagtgc
    25441 tccgacaaca ataatccctg aaatcgcgtt ggtgaccgac atcaacggtg tatgcagcgc
    25501 gtgcgatacg ttccacacca cgtaataacc gacaacgcag gccagcgcga aaacggtgaa
    25561 gtgcccaaga aactctttcg gcgcaacgct tgccagccag ccgaaaagga tgattgccag
    25621 cgccatcaac gcgtatttac gccacggtga acaggcacat ttttcctcag ttttcacttc
    25681 cggtgccgct ttttgtgcca cctgcggctg agctgatacc tgaatcggcg gtgccggcca
    25741 ggtaatttcg cccgcacgga tcacagtcac gccgcgaatc accacatcat caaaatcaac
    25801 agtgatgtta ccgtttttct ctttgcacaa cagtttcagc agattaacga ggttagtgcc
    25861 gtaaagctgt gaggattgcg tcggcagacg gcccggaaga tcggtatagc caatcacttt
    25921 gacaccattt tccgtagtga agatttcacc cggcacggtg tattcacagt tgccgccgtt
    25981 ttgggctgcc aggtcgacaa tcacactgcc cgccttcatg gagtcaacca tttcacgggt
    26041 aattagcttc ggcgctggtt tgcctggaat aagcgcggtg gtgacaatga tatcgacctc
    26101 ttttgcctgg gcggcaaaga gttccatttc cgctttgatg aacgcgtccg acatcacttt
    26161 ggcatagcca tcgccgctgc ccgcttcctc tttaaaatcc agctcgagga attccgcgcc
    26221 catactttga acttgttctt tcacttccgg gcgggtgtcg aatgcacgca caatcgcgcc
    26281 gagactgttt gctgcgccaa tggcggccag acctgcaaca cccgcaccaa tcaccatcac
    26341 ttttgccggt ggcactttcc cggccgcagt aatttgcccg gtaaagaagc acccaaattc
    26401 atgtgccgct tcaacaatgg cgcgataacc ggcgatattc gccatcgagc ttagtgcgtc
    26461 cagcgattgt gcgcgtgaga tacgcggcac agagtccatc gccatcacgg tcacgttacg
    26521 ttccgcaagt ttttgcatta attccggatt ctgcgcaggc cagataaaac tcaccagcgt
    26581 tgtcccagga ttcagtaacg caatttcatc atctaacggc gcattgacct tcagaatgat
    26641 ctctgactgc cagacgctat tcccttctac aatttcagcg cccgcttgca caaacgcttt
    26701 atcgtcaaaa cttgccagtt gacccgcgcc gctctctacc gcgacggtaa accccagttt
    26761 cagcagctgt tccactgttt ttggcgttgc tgcaacacgg ggttcattgg ttaaccgttc
    26821 tcttggtatg ccaattcgca tgatattccc ttccatcggt tttattgatg atggtttgcc
    26881 tgtgtcagga gccacacaag ccgctcatgt acgagctaaa tgttactccg ttaaaataaa
    26941 ttagtaacaa acgccctata acgtactgaa aattatgcct gtgatctagc gccaaaaatc
    27001 agtatttcgg cgtgaactcg caaaatatta acgattcagc cgtgatagtg ggataaacac
    27061 cttagaacgc cggataaaga ctgataattg tcttcgacgg tcgggtaaaa cgagacatcg
    27121 ccccggcacg aatcactact taacattaaa ttaacttata caattcagtt gcttcagtag
    27181 taatgatgct gatacggctg ttttttaagc atagacggtc atttgagcag gattaaaatt
    27241 ggcttaagga atgtgatatg aaaaatgacg cagacagtta caccgtttaa atgcaataat
    27301 cagccacgtt tctcgttaat aacaatacca gtacctggtt tgcgcaaggc gaaggattat
    27361 ttttatgaag cttaagaaca ccctcctggc gtcggcactg ctttctgcta cggcattctc
    27421 cgttaacgca gcaacagaac tgacaccgga gcaagcggca gcggttaaac cttttgaccg
    27481 tgtagtggtt accggtcgtt ttaatgctat tggcgaagcg gtgaaagccg tttctcgtcg
    27541 cgcagataaa gaaggtgccg cctcttttta tgttgtcgac acttctgatt ttgggaacag
    27601 cggtaactgg cgtgtggtcg ctgacctcta taaagccgat gctgaaaaag cagaagaaac
    27661 aagtaatcgc gtaattaacg gtgttgtcga actgccgaaa gatcaggctg ttctgattga
    27721 accgtttgac acggtcaccg tccagggctt ctatcgtagc cagccagaag tcaatgatgc
    27781 catcaccaaa gcggcaaaag cgaaaggtgc ctactctttc tacatcgttc gtcaaatcga
    27841 tgccaaccag ggcggcaacc agcgtattac tgcattcatc tataaaaaag atgctaagaa
    27901 acgtatcgtc cagagcccgg atgtgatccc ggcagattcc gaagcaggac gtgcagctct
    27961 ggctgccggt ggcgaagccg cgaagaaagt tgagatcccg ggtgttgcga ctaccgcatc
    28021 accaagttct gaagtcggtc gcttctttga aacccagtca tcaaaaggcg ggcgttacac
    28081 tgtcacgctt ccggatggca ctaaagtcga agaactgaac aaagcgacag cagcgatgat
    28141 ggtcccgttc gacagcatca aattctctgg caactacggc aacatgaccg aagtctccta
    28201 tcaggttgcg aaacgagccg cgaagaaagg tgctaaatat taccacatca cccgccagtg
    28261 gcaggaacgt ggtaataacc tgaccgtcag tgcagatctg tataaataac tattcagtta
    28321 ctcttataag gcggcttgat gccgcctttc gcatttttag tcacaaattt ccccgcgacc
    28381 attgcatgca gacacatctc ttcgtaaaat cccgcgcttc agtggcaaac tccattttta
    28441 tgcgttttgc cctaatattt attcttctta tcacgtttta atcactggat atcgatggaa
    28501 aagaaactgg gactgagcgc actcaccgcg ctggtattaa gctcaatgct gggcgcgggt
    28561 gttttcagtc tgccgcaaaa tatggcggca gttgccagcc cggcagcact gctcatcggc
    28621 tggggtatta ctggcgctgg cattttattg ctggcctttg ccatgctgat cctcacgcgc
    28681 attcgtcccg aacttgacgg cggtatcttt acctatgccc gcgaagggtt tggcgagcta
    28741 atcggctttt gttccgcatg gggatactgg ctgtgcgcag tcatcgccaa cgtctcctat
    28801 ctggtgatcg ttttttccgc gttaagcttt tttacggaca cgccggaatt gcgcctgttt
    28861 ggcgatggta atacctggca atcgatagtc ggtgcatcgg ctttattgtg gattgttcac
    28921 tttttgatcc tgcgcggggt gcaaaccgct gccagcatta acctggtggc gacactggca
    28981 aaattgttgc cgctgggtct gtttgttgtg ctggcgatga tgatgttcaa actggatatc
    29041 ttcaagctcg acttcaccgg acttgcactt ggcgtacctg tttgggaaca ggtgaaaaac
    29101 accatgctga tcaccctgtg ggttttcatt ggtgtggaag gtgccgtggt tgtttctgcg
    29161 cgtgcgcgta ataaacgtga tgttggcaaa gcgacactgc tggcggttct ctccgctctg
    29221 ggcgtttact tgctggtaac gctgctttcg ctgggagtgg tggcacgtcc tgaactggct
    29281 gaaattcgta acccgtcaat ggccggtctg atggtggaaa tgatgggacc gtggggcgaa
    29341 atcatcatcg ctgccggttt gatcgtttcc gtttgcggcg cgtacttaag ctggaccatc
    29401 atggcggcag aagttccgtt cctggctgcc actcataaag cattcccacg catttttgcg
    29461 cgccagaacg cacaagctgc gccatccgcc tcactgtggt tgacaaacat ctgcgtgcaa
    29521 atttgtctgg tactcatctg gctgaccggt tccgattaca acacgttgct gaccatcgcc
    29581 tcagaaatga ttctggtgcc ctatttcctg gtcggcgcgt tcttgctgaa aatcgcaacc
    29641 cgtccactgc ataaagcggt aggtgtcggt gcctgcattt atggcttatg gttattgtac
    29701 gcctctggtc caatgcactt gctgttatcc gttgttctgt atgcaccggg gctgctggtt
    29761 ttcctgtatg cgcgcaaaac gcatacacat gataatgtac tgaaccgtca ggaaatggtg
    29821 ctgattggca tgctgttaat tgcctcagtt ccggcgacct ggatgctggt gggataacgt
    29881 tcatcccatc gttggatagc aaaggagata acgatgggta aaacccagcc cttgccaata
    29941 ttaattactg gcggaggtcg tcgcatcggc ctcgccctcg catggcattt cattaatcaa
    30001 aagcaaccgg tgattgtcag ctatcggaca cactatccag ccattgatgg actgattaat
    30061 gcaggtgcgc agtgtattca ggctgatttt tcgaccaacg acggtgtgat ggcgtttgcc
    30121 gatgaagtac taaaaagcac ccatggtctg cgtgctattt tgcataacgc cagtgcgtgg
    30181 atggcggaaa aaccgggtgc gccactggcc gacgtactgg cttgcatgat gcagatccac
    30241 gttaataccc catacctgct caaccatgcg ctggaaagat tactgcgtgg gcacggacac
    30301 gccgccagcg atatcattca ctttaccgat tatgtggtgg agcgcggtag cgacaaacat
    30361 attgcgtatg ctgcaagcaa agcggcactg gataatatga cccgctcgtt tgcccgcaag
    30421 ctggcaccgg aagtgaaagt gaattctatt gcgccatcgc tgatcctgtt taatgaacat
    30481 gatgatgccg aatatcgaca acaggcgctg aataaatcat tgatgaaaac cgcgcctggc
    30541 gagaaagaag tgatcgacct ggtcgattac ttacttacca gttgctttgt caccggacgc
    30601 agtttcccac ttgatggcgg tcgtcatctg cgttaatgca gctttatcca gcatataatc
    30661 aacacccatg cgcttatccc ccaacacgca acagaaccaa caaaagcggc gggcaagcgc
    30721 atcattccgg tgaaatacca cagcgacacc aggtaaacaa aatagggaat aatcgaccac
    30781 atactaaaaa tgatggttgc gcgtaaggct tcaatgccgc gttcgctggc aacaatataa
    30841 tgcgcgataa gcgcaaaggt cgggaaaagt ggaatcagcc cggcgatata ataatttttc
    30901 gtttttgcta aaacaccaat caacagcact accagcgcac cgagcgcggc tttgattacc
    30961 agccccatct ttttacctta acacttccat aacaagtcat cagtagaata cctgatgaaa
    31021 acttgtttag aaacgattga tagtaagtaa aaacagcgcg gtgtattgtg acgtttttat
    31081 atctaccgtg aatgttatga acactatcgt atttgtggaa gatgatgcgg aagtcggttc
    31141 actgattgcc gcgtacctgg caaaacatga tatacaggtt accgtagagc cgcgcggcga
    31201 ccaggccgaa gaaaccattt tgcgagaaaa tccggatttg gtgttactcg acatcatgct
    31261 accaggcaag gacggcatga ccatttgtcg tgatttacgc gcaaagtggt ccggaccgat
    31321 tgttcttcta acctctctcg atagcgatat gaaccacatc ctggcactgg aaatgggtgc
    31381 ctgcgactat attctcaaaa cgacgccccc tgctgttttg ttagcgcgtt tacgtttgca
    31441 tttgcgtcag aatgagcaag ccacgctgac caaaggtctt caggaaacgt cactgactcc
    31501 ctacaaagcc ctgcatttcg gcacgttgac catcgatcct atcaaccgcg tagtcaccct
    31561 ggctaacact gaaatctcgc tctcgacagc tgatttcgaa ttattgtggg aattagctac
    31621 ccatgccggg caaatcatgg accgcgatgc attgctgaaa aatttacgcg gcgtcagtta
    31681 tgacggcctg gatcgtagcg tggacgtggc tatttcgcgg ttaagaaaaa aactgctcga
    31741 taacgccgct gaaccttatc gcattaaaac tgtgcgtaac aaaggctatc tttttgcgcc
    31801 tcatgcatgg gaataagcga tgaaaaaact gtttatccag ttttacctgt tattgtttgt
    31861 ctgcttcctt gtgatgtctc tgctggttgg gttggtgtac aaatttaccg ccgaacgcgc
    31921 gggcaaacag tcgctggatg atttgatgaa cagttcgctg tatctgatgc gcagcgaatt
    31981 gcgtgagatc cccccacatg actggggtaa gacgctgaaa gagatggatt taaatctctc
    32041 tttcgatctg cgagtcgagc cactgagtaa ataccatctt gatgatattt ccatgcaccg
    32101 gctgcgtggc ggcgaaattg tcgccctgga cgatcagtac acttttttgc agcgtatccc
    32161 gcgcagccac tacgtgctgg cagttggtcc tgttccttat ctttattatc tccatcagat
    32221 gcgattgctg gatatcgccc tgatcgcttt tattgctatt tccctcgcct ttccggtgtt
    32281 tatctggatg cgtccgcact ggcaggatat gttaaaactg gaagcagcgg cgcaacgatt
    32341 tggcgatggg catctcaatg aacgtatcca ctttgatgag ggttcgagct ttgaacgact
    32401 tggtatcgca tttaaccaga tggcggacaa tatcaacgcc ttgattgcca gcaaaaaaca
    32461 gcttattgac ggtatcgctc acgaactgcg aacaccgtta gtgcgcctgc gttatcgact
    32521 ggagatgagc gataacctga gcgccgccga atcccaggcg ttgaatcgtg atatcagtca
    32581 acttgaagct ttaattgaag agctgctgac ttatgcccga ctcgatcgcc cacaaaacga
    32641 gcttcatctt agcgaaccag acctgccgtt gtggctgtca acgcatctgg cagatattca
    32701 ggcagtaacg cccgataaaa cggtacggat aaaaacgctc atgcaaggcc attatgcggc
    32761 gttggatatg cgcttaatgg agcgcgtgct ggataatttg ctcaataatg ccctgcgcta
    32821 ctgccattca acggttgaaa ccagcctgct actgtcgggg aatagagcga cattaattgt
    32881 tgaggatgat ggcccaggga ttgccccaga aaaccgcgaa catatctttg aaccttttgt
    32941 tcgcctcgat cccagccggg atcgctcaac cggcggctgc gggctggggc tggcaattgt
    33001 ccactctata gcactggcaa tgggcggtac ggttaattgt gacaccagcg aactgggtgg
    33061 tgcccgcttc tcgtttagct ggccgttatg gcataacatc ccgcaattta cctctgcttg
    33121 acactacgcg cacggtggtc aagtcaccac cactgtgcta taaattaagg atgttgtaac
    33181 taaagtggtt aatattatgg cgcgttacga tctcgtagac cgactcaaca ctacttttcg
    33241 ccagatggaa caagagctgg ctgcatttgc cgctcatctt gagcaacaca agctattggt
    33301 tgcccgcgtg ttctctttgc cggaggtaaa aaaagaggat gagcataatc cgcttaatcg
    33361 tattgaggtg aaacaacatc tcggcaacga cgcgcagtcg caggcgttgc gtcatttccg
    33421 tcatttattt attcaacaac agtccgaaaa tcgcagcagc aaagcagctg tccgtctgcc
    33481 tggcgtgttg tgttaccagg tcgataacct ttcgcaagca gcgttggtca gtcatattca
    33541 gcacatcaat aaactcaaga ccacgttcga gcatatcgtc acggttgaat cagaactccc
    33601 caccgcggca cgttttgaat gggtgcatcg tcatttgccg gggctgatca cccttaatgc
    33661 ttaccgcacg ctcaccgttc tgcacgatcc cgccacttta cgctttggtt gggctaataa
    33721 acatatcatt aagaatttgc atcgtgatga agtcctggca cagctggaaa aaagcctgaa
    33781 atcaccacgc agtgtcgcac cgtggacgcg cgaggagtgg caaagaaaac tggagcgaga
    33841 gtatcaggat atcgctgccc tgccacagaa tgcgaagtta aaaatcaaac gtccggtgaa
    33901 ggtgcagccg attgcccgcg tctggtacaa aggagatcaa aaacaagtcc aacacgcctg
    33961 ccctacaccg ctgattgcac tgattaatcg ggataatggt gcgggcgtgc cggacgttgg
    34021 tgagttgtta aattacgatg ccgacaatgt gcagcaccgt tataaacctc aggcgcagcc
    34081 gcttcgtttg atcattccac ggctgcacct gtatgttgca gattaacgcc cggctttcat
    34141 actgccgacc atctgttctg gccgtaccca gctgtcaaac tcggcttcgc taagataccc
    34201 cagcgcaagg gccgcagctt ttaaggtcag cccttcttta tgcgcttttt tggcgatctc
    34261 cgcggctttg tcataaccaa tgtgggtgtt aagcgcagtc accagcatca gcgattcatt
    34321 gagtaattga ttgattcgct cacgattcgg ttcaataccc actgcgcagt gtttgttaaa
    34381 actttccatg ccatctgcca gcaagcgcac cgattgcagg aaattgtgga tcaccattgg
    34441 acggaagacg ttcagttcaa agttaccgga agcgcccccc atgttgatcg ccacgtcgtt
    34501 ccccatcacc tgacagcaga gcatggttaa tgcctcacac tgtgttgggt tcactttccc
    34561 cggcatgatt gagctgcccg gctcattttc cgggattgag atttcaccaa ttccgcagcg
    34621 cgggccagag gccagccagc ggacatcatt ggcgattttc atcagtgacg cagccaaccc
    34681 tttcaacgcg ccgtgcgcct gaaccagggc atcacaggtc gccagcgctt caaatttgtt
    34741 cggcgcggta acaaacggtg cacaggtaat gactgccagt tcatctgcta cgcgacgcgc
    34801 atactccgga tgggtattta gtccagtacc caccgctgta ccgccaagag ccagttccgc
    34861 tacgtgaggc aggctgtatt cgatatgttt gagattatgc tcgagcatcg ctacccagcc
    34921 ggaaatctcc tgccccagtg ttagcggggt ggcgtcctgc aagtgggttc gaccgatttt
    34981 tacgatatcg gcaaatgcac gcgatttttc actcagcgtc tgtgtcaggg ttttaagctg
    35041 cggaatgagt tgcttgcgca gcgccagcag cgccgcaacg tgcatcgccg tcggaaagac
    35101 atcgttggaa ctttggcttt tgttcacgtc gtcgttaggg tgaactttac gttccatccc
    35161 gcgcacgccg ccgagtaatt cactggcccg gttagccagc acttcattca tgttcatgtt
    35221 actttgcgtg ccggagccgg tctgccagat agccagcggg aattcgtcgt catgctgtcc
    35281 tgccagtact tcatccgccg cctgccgaat ggcgctcgct ttctcttcag acaacaagcc
    35341 taaatcttca ttaacttttg ccgctgcgcg cttggttagc gccagcgcat gaatcagtga
    35401 ggtgggcatt ttctccgtcg aaatgcggaa atgctccagc gagcgttgag tttgtgcgcc
    35461 ccacagctta tctgccggga catcaatcgc ccccatcgaa tctttttcgc tgcgtactgt
    35521 attcatggcc tactcctcac ctgattaatt ttttctttct gttttgcttt cgttaagtaa
    35581 cttttagctc actcattatt tactatttga taacaaatgt ttggtctttc gtgccatgta
    35641 aaaaaaccgc cccgaagggc ggctctgttt atttcacaca gcgggtgcat tgtgtgagtt
    35701 gtatctgctg gaagaagtca tttcctttat catccacaag gataaacgcc gggaaatctt
    35761 ccacttcaat tttccagatg gcttccattc ccagttccgg atattcaaca cattccaggc
    35821 tcttaatact tccctgcgcc aatacagcgg ccggaccacc gatactgcca aggtagaagc
    35881 caccgtgttt tttacaggca tccgtcacct gctggctgcg gttgcctttc gccagcatga
    35941 tcatacttcc gcccagcgct tgcagttgat cgacataaga atccatccgt ccggcggtcg
    36001 ttgggccaag agaaccggag gcataacctt ccggcgtttt ggccggaccc gcgtagtaaa
    36061 tcggatgatc tttgatgtac tgcggcagcc cttcaccgtt atccatccgc tctttcagtt
    36121 tggcgtgagc aatatcacga ccgacgataa tcgtgccgtt aagcgataag cgtgtagaaa
    36181 cgggatactg cgacaactgt gcgaggatct ctttcatcgg acggttaagg tcaacgcgca
    36241 ccgcttcgcc ttctcccgct ttgcgcagct cttccgggat atatttgcct ggattatgtt
    36301 ccagtttttc gatccagatc ccctgacggt tgatcttcgc tttgatatta cggtcagcag
    36361 aacaggagac gcccataccg accgggcagg atgcgccgtg acgtggcagg cgaatcacgc
    36421 ggatgtcgtg agcgaagtat ttaccgccaa actgcgcacc cagaccaaga ttttgcgctt
    36481 cgatcagcaa ttctttttcc aattccacat cgcggaacgc ctgaccgtgc tcattccctt
    36541 ccgttggcag ttcatcatag tatttcgcgg aagccagttt caccgtttta aggttcgttt
    36601 ctgcagaagt tccaccaata acgaacgcaa tatgatacgg aggacaggcc gccgtaccca
    36661 gcgtgcgcat cttctcaacc aggtaatttt tcagtttccc cggcgtcagt aacgctttgg
    36721 tttcctgata gagatacgtc ttgtttgccg aaccaccacc tttggcgata cagaggaatt
    36781 tgtattcgtc gccatcaacg gcataaagat cgatctgcgc tggcagattg gtgccggtat
    36841 tcacttcttt atacatatcc agcggcgcgt tttgcgagta gcgcagatta tcttcgatat
    36901 aagtgttata gacaccgcgc gccagcgccg cttcatcacc accaccggtc catacacgct
    36961 gccctttttt accaacaata atcgcggtgc cggtatcctg acaggttggc agaacgcctt
    37021 tcgccgcgat gtcggagtta cgcaggaatt gcagcgccac atatttatca ttttcgctgg
    37081 cctccgggtc acgcagaatg tcggccacct gttgttggtg cgccggacgc agcatgaacg
    37141 acgcatcatg aaacgcctgg cgcgccaaca gagttaacgc ttcgggtgcg actttcaaaa
    37201 tctcctgccc ttcaaattca gataagctaa cgtgttcgct ggttagcagg taatactcag
    37261 tatcatcttt tttgagtgga aaaggagcct gataatgaaa gggtttgttt gacattgttt
    37321 tctcacttac tgcctggttt ggttatgctc tgggcgggtg ttccgttgcc ctgttaaaag
    37381 cgagtaacaa tatcctacgc acttttttaa caaaaactga gactagtacg actttttgcg
    37441 gctccaggtt acttcccgta ggattcttgc tttaatagcg ggattaattt ccacattaaa
    37501 acagggattg atcatgcaaa aactcattaa ctcagtgcaa aactatgcct ggggcagcaa
    37561 aacggcgttg actgaacttt atggtatgga aaatccgtcc agccagccga tggccgagct
    37621 gtggatgggc gcacatccga aaagcagttc acgagtgcag aatgccgccg gagatatcgt
    37681 ttcactgcgt gatgtgattg agagtgataa atcgactctg ctcggagagg ccgttgccaa
    37741 acgctttggc gaactgcctt tcctgttcaa agtattatgc gcagcacagc cactctccat
    37801 tcaggttcat ccaaacaaac acaagtctga aatcggtttt gccaaagaaa atgccgcagg
    37861 tatcccgatg gatgccgccg agcgtaacta taaagatcct aaccacaagc cggagctggt
    37921 ttttgcgctg acgcctttcc ttgcgatgaa cgcgtttcgt gaattttccg agattgtctc
    37981 cctactccag ccggtcgcag gtgcacatcc ggcgattgct cactttttac aacagcctga
    38041 tgccgaacgt ttaagcgaac tgttcgccag cctgttgaat atgcagggtg aagaaaaatc
    38101 ccgcgcgctg gcgattttaa aatcggcgct cgacagtcag cagggtgaac cgtggcaaac
    38161 gattcgttta atttctgaat tttacccgga tgacagcggc ctgttctctc cgctgttgct
    38221 gaatgtggtg aaattaaatc ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc
    38281 ttacctgcaa ggcgtggcgc tggaagtgat ggcaaactcc gataacgtgc tgcgtgcggg
    38341 tctgacgcct aaatacattg atattccgga actggttgcc aatgtgaagt tcgaagccaa
    38401 accggctaac cagttgttga cccagccggt gaaacaaggt gcagaactgg acttcccgat
    38461 tcctgttgat gattttgcct tctcgctgca tgaccttagt gataaagaaa ccaccattag
    38521 ccagcagagt gccgccattt tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc
    38581 tcagcagtta cagcttaaac cgggtgaatc agcgtttatt gccgccaacg aatcaccggt
    38641 gactgtcaaa ggccacggcc gtttagcacg ggtttacaac aagctgtaag agcttactga
    38701 aaaaattaac atctcttgct aagcttatta aaggcttata acaccttcag gcggccagtc
    38761 cgcctgattt cattttatgg ataatcatta tgaataaatc gctggtagcg gtaggcgtca
    38821 ttgttgcgct aggcgtagtc tggacaggcg gcgcatggta tacaggcaag aagattgaaa
    38881 cccatctcga agacatggtc gcgcaggcga acgcgcaact caaactgacc gctcctgaat
    38941 ccaacctgga agtgagttat caaaactatc atcgcggcgt attcagcagt cagctgcaac
    39001 tgttggtgaa acccattgcc ggaaaagaaa atccgtggat taaaagcggt cagagcgtca
    39061 tcttcaacga atcggttgat catggtccct tcccccttgc ccagcttaaa aaactgaacc
    39121 tgatcccgtc gatggcatca attcaaacca cgctggttaa taacgaagta agcaaaccac
    39181 tgtttgatat ggcaaaaggt gaaacgcctt ttgagattaa ctcgcgcatt ggttacagcg
    39241 gtgattccag ttccgatatt tcgctcaagc cactaaatta cgagcaaaag gatgaaaaag
    39301 tcgcctttag cggcggcgag ttccagttaa atgcggacag agacggcaaa gctatctccc
    39361 tttccgggga ggcgcaaagt ggtcggatag acgcggttaa cgaatacaac cagaaagtac
    39421 agttgacctt taataatctg aaaaccgacg gttccagcac gctggcaagt tttggtgagc
    39481 gcgtaggaaa ccaaaaactg tcactggaaa aaatgaccat ttcagtggaa ggcaaagaac
    39541 tggcactgct ggaaggcatg gagatcagcg gtaaatcgga tctggtcaat gacggtaaaa
    39601 cgatcaatag ccaactggat tactcgctaa acagcctgaa ggtacagaat caggatctgg
    39661 gcagcggcaa gctgacttta aaagtcggcc aaattgatgg cgaagcctgg catcagttta
    39721 gccagcaata taacgcgcaa actcaggcgc tgctggcaca gccagaaatt gccaacaatc
    39781 ccgaacttta tcaggagaaa gtgacggaag ccttctttag cgccctgccg ctgatgttga
    39841 aaggcgatcc ggtgattact atcgcgccgc taagctggaa aaacagtcag ggtgaaagtg
    39901 cgctgaatct gtcgctgttc ctgaaagatc cggcaacgac taaagaagcg ccgcaaacgc
    39961 tggcgcagga agtagatcgt tcggttaaat ctctggatgc gaaactgacc attccggtgg
    40021 atatggcaac tgagttgatg actcaggtag cgaagctgga aggttatcag gaagatcaag
    40081 cgaaaaaact ggcgaaacag caagttgaag gtgcatcagc aatggggcag atgttccgtc
    40141 tgaccacctt gcaggacaat accatcacca ccagcctgca atatactaac ggtcagataa
    40201 cgttaaacgg gcagaaaatg ccactggaag atttcgttgg tatgtttgca atgccggcat
    40261 taaatgttcc ggtcgtaccc gctattccgc agcagtaatt cacagccaaa acgccggaga
    40321 tttttctctc cggcgttatt ttttacttca gcataaagtc atactgaata ctcattttca
    40381 catcccagta attcaggcga tcgcccagcg tactgccatc gcgcttttcc cgccctttat
    40441 cggcagcaag aaagaaattc atcccttgta acggcccttc gctgacccga tagttcatac
    40501 cacctaagat acccgcgcct tcaatccgca catctcgttt actggattca tagccgcgag
    40561 tcacaatcgg tgcaaatgtc agtgtaaatt gggagttaaa cgatagttga cgcccaattg
    40621 ccaggactgc attccttcac ggttacgatc aagacttaat gaaaagggat agccaatgtc
    40681 ggtatccacc acggcaccat tcattgcatg gcgcaaataa ccaccactgc caaatacgct
    40741 tccccacggc gcattccagg tcaattgtcc actaaccaac gccgtttcat tgggctggct
    40801 ggtattacga cttaaacctt cgagttgcgc gtaaaagccg agcaactccc ctttcaacgt
    40861 ataattcggg tcgagcggta tgcttttctc gagaatactt tgtatctgat tggaaacatc
    40921 tttctgtacc gatgccccca gagaatagcg actggtatca tcatgccaac tggcggctaa
    40981 aaaagaacga gcgcgatgtt tatcgtattt tcccgtctgg ctgttataca ccccttcatc
    41041 acgaataccg gttgttgaat cagtccataa aaatactcgt gaacgccaca ttccccacag
    41101 atcaatattt ggcgttaaag cccaataacc taatgctcct tcagaaactg tcggtaatgt
    41161 acgttgcgaa ctgccagcca ttaacggaat acgataaggt aagctaccat aactataatc
    41221 cgtcccgaag cgacctaatt taagcaggct attttcactg ggttttattt ttactgcccc
    41281 gagggcaaag ccaaaacttt tatcaccatc aagataccac cgggtactca tatcagcacg
    41341 agcacctaat ttataaacat aatacgctcc gccttcaacg ccaataatat tggaataata
    41401 accactattg aaatcgagca atcctccctg tacccatgcg taaatatctc ttcccgcgcc
    41461 agcactgggc ttatcagccc tgcgcaattc gtttttcagt ctgatgcgta gtcccgcttc
    41521 atcgtcagcc agttgcgccg cgtaagcaga ggtaaggcca gaggcagccg tcaggcaaat
    41581 aacggccatg gccactattt ttctcatagt tgcacctttg atgttctgat tatttttatt
    41641 gaatattaat tagtgatatc gctgattaat tgctgctgca cttttttacg attatcaatt
    41701 tcaaccacga tttctttgaa ttttttatcc gtgagcggat aaaaccagat aataacgaat
    41761 gccagtagca taaatccgca aggtactaag gcaattgatg tgcggatgcc cataattact
    41821 tccggcgttt gcacctgatt ggcgatatat ccgcttaatc ccaaaataaa ggcaggaatt
    41881 gaaccaccga ttgcctgacc gcatttacgg gtaaatgaga atagtgaata ggtgagccct
    41941 tcaattcgca cgccggtcag gtattcaccg tattctacgg tatcagcttc cagcgcccac
    42001 atcacggtca tggtaacgcc ctgaccaatt gaggcaatgg ctaacgcaac caacgccacc
    42061 ggcagcgacc agacggaaac ccagaagaac agcagataac cgcaggttcc cagcaaagcg
    42121 ccaatcagga aggtattctt tttaccgatc ctcgcgacca tccccggcac caacggtgcc
    42181 gatgccacag taccaaccag gttttgcacc agtaccagca cagtgaacag cccggtatca
    42241 tttaacacat agcgcacgta gaacaacgac gaggcgctga ccgcgaaggt cgaaatcagc
    42301 acacacagcg caccgatgca caacataaac agcgggcgat tccgtttcag ggtttgcaga
    42361 ctgatcttca atgacggctg cgccacgata cgtaccacat tctcacgcgt cgatttgaag
    42421 cagatgaagt aaagcaccat tccggcaatc gccagcacaa tcgtccagaa atggtatacc
    42481 gacaccatct cttccgggct ggagttctta atgctcggcc ctatcagaaa tgccaggcag
    42541 acaaaggtca atgaagcggc aatcccacga gccgcgccca gacgggcgcg ggattgtggt
    42601 tgttgggtca tcgcggtagc aagtgaacca taaggaatat tcaccaggct gtagcaaagc
    42661 ccgaggccca tgtaggtcaa atatgcatac accactttgc taccatggct ccagtcggtc
    42721 ggcacccaga ataccagcac gctgaagatc attaacggcg cagtaccgaa gagtaaaaac
    42781 gggcggaatt ttccccagcg ggtattcaca ctgtccacca ctcgtccggc aaagacgtcg
    42841 gcgaaggcat cgaatacccg caccagtaac agcatggtgc ccgccgcagc ggcaccgacg
    42901 ccagcgacgt cggtgtagta actcaacagg aagagcgccc ccattgcgaa ggcgaagtta
    42961 ttggcgatgt caccaaggct gtagccgacg atggtgcgcc aggagagttg ttgattcatt
    43021 gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc agtccagcgt ttttgcagca
    43081 gaaaagccgc cgacttcggt ttgcggtcgc gggtgaagat ccccttcttg ttaccgccaa
    43141 cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt ccatacctgt tcaccgacga
    43201 cggcgctgac gcgatcaaag acgcggtgat acatatccag ccatgcacac tgatactctt
    43261 cactccacat gtcggtgtac attgagtgca gcccggctaa cgtatccacg ccgtattcgg
    43321 tgatgataat cggctgatgc agtttctcct gccaggccag aagttctttt tccagtacct
    43381 tctctgccgt ttccaaatcg ccgctttgga cataccaacc gtaataacgg ttcaggcaca
    43441 gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc gcagaacatt acattgacgc
    43501 aggtgatcgg acgcgtcgga tcgagtttac gcgttgcttc cgccagtggc gcgaaatatt
    43561 cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat actccacatc accacgcttg
    43621 ggtggttttt gtcacgcgct atcagctctt taatcgcctg taagtgcgcc tgctgagttt
    43681 ccccgttgac tgcctcttcg ctgtacagtt ctttcggctt gttgcccgct tcgaaaccaa
    43741 tgcctaaaga gaggttaaag ccgacagctg cagtttcatc aatcaccacg atgccatgtt
    43801 catctgccca gtcgagcatc tcttcagcgt aagggtaatg cgaggtacgg taggagttgg
    43861 ccccaatcca gtccattaat gcgtgatcgt gcaccatcag cacgttatcg aatcctttgc
    43921 cgcgcaaatc cgcatcttca tgacggccaa agccagtaaa gtagaacggt ttgtggttga
    43981 tcaggaactg ttcgcccttc actgccactg accggatgcc gacgcgcagc gggtagatat
    44041 cacactctgt ccggcttttg gctgtgacgt acagttcata gagataacct tcacccggtt
    44101 gccagaggtg cggattcacc acttgcaaag tcccgctggt gccccttgtc cagttgcaac
    44161 cacctgttga tccgcatcac gcagttcaac gctgacatca ccattggcca ccacctgcca
    44221 gtcaacagac gcgtggttac agtcttgcgc gacatgcgtc accacggtga tatcgtccac
    44281 ccaggtgttc ggcgtggtgt agagcattac gctgcgatgg atcccggcgt agttaaagaa
    44341 atcatggaag taagactgct ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg
    44401 gatagtctgc cagttcagtt cgttgttcac acaaactgtg atacgtacac ttttcccggc
    44461 aataacatac ggcgtgacat cggcttcaaa tggcgtatag ccgccctgat gctccatcac
    44521 ttcctgatta ttgacccaca ctttgccgta atgagtgacc gcatcgaaac gcagcacgat
    44581 acgctggcct gcccaacctt tcggtataaa gacttcgcgc tgataccaga cgttgcccac
    44641 ataattacga atatctgcat cggcgaactg atcgttaaaa ctgcctggca cagcaattgc
    44701 ccggctttct tgtaacgcgc tttcccacca acgctgctca attccacagt tttcgcgatc
    44761 cagactgaat gcccacaggc cgtcgagttt tttgatttca cgggttgggg tttctacagg
    44821 acgtaacata agggactcct cattaagata aaaatactgg tcaaccttta atctgattag
    44881 ttaaccaatt ctgtgttgca cacagttatc tgtgagaaac ccatggaaga gaaatggaaa
    44941 taaaaagaac tgacaccgcg atcaaaaaac caaaaactgc aatacccaca gcgtgatata
    45001 gatcgcatta atctttaaaa cggcgttgac agaagccata aaataagcaa ccgaacgttc
    45061 gttcgagcat gttgggaacg ataaactctc atgacatatg gctacggtga atattttagg
    45121 gaggaattcc ccgctccggc gagattgtat tttgtacagg gaaattaaag tatgaaacgc
    45181 gggctcagtt gtcaccagcc cgcggaaaga gagagagaat caggatgcgg ttaagatacc
    45241 gccaatcatc gcgcgtaatc cctgagcaaa cgacgcttca cgcgccaggt cttccgcttc
    45301 gatatcggcc agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca ttgccgtgtt
    45361 gatgtctggg cttatctcgc ctttttgctg ggcatcacgc atccgctgag caacaaattc
    45421 agtgatcgtc atatgcttat ttttaagcat ggcggcgacc tgtgggttac gcatcccttc
    45481 cgccataatt tcgaccacca gcgcccgttg cccaaaggct tcatgggtga gagagacaat
    45541 agactcgacc atatagtcaa caaaatgaat cccttcaatc ggttcccgga aacgggccag
    45601 cgccctctcc tggtcctgta agataatggc ctgaatcaag gcttctttgg agatgaaatg
    45661 gtgatagagc gtcccgggac taatggcgca ggatttacag atggctttca tcgaggcact
    45721 gtgaaatcca ttttctgaaa aaatctctct ggcagcattg aggatccggg tccgtgtcgg
    45781 ttgtgcttca gtctgcatgt tatccatcat taaaccatta tagttagcgt gaataactag
    45841 tttaccgcgt tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa ggcacactct
    45901 attcgtatgc ttttcatcca gctattggtt aaccaatggc cgtgatttgg ctgccagttg
    45961 tttagcagag acgttgatga attgcacacg ctatcccggt aaacaacaca tacggctgtt
    46021 taccggttcg ttagtgtatt aattgagctc ctgtacccca ccaccggaga cggtgagaat
    46081 ttgtccgctt acccagctgg ctgcaggcga gcaaaggaac agcgctgcgt tagcaatatc
    46141 ttgcggttgg cccagacgtc tgatcggcgt gtgctgtaac attttttgtt caatttctgg
    46201 tgtaataacg gatttcaggg catcggttaa tattgccccc ggcgcaatgc cattcacccg
    46261 aatatttttt tcacccaggt caaatgccat atttctgacc agatgactgg ccgcagcttt
    46321 agatgatgca taggaagtca tgtttatatt tttattttct gccgccatag aagtgatggt
    46381 cagaataacg ccaccgccat ttttttccat ttctggcgca acaagttgtg acagatggaa
    46441 aaaagaaaac acattcagtt cataagcacg gcgaaaatcc gccattggca tatcaaacgg
    46501 tttaggtcca ccgccaccgg cgttgttaac cagaatatca actttaccca gcttactgat
    46561 agcaaagtct gccagtgcag agagttcctg ttcggaagta atatcacaac ggcaggcaaa
    46621 tgcctgacca cccagttgtt gaatttcgtc tacaacatgg ttagctgcgt cggcgttaat
    46681 atcactgacc accacagatg cgccagctgt cgcgaatgta atggcgattt ctttaccaat
    46741 acctgcaccc gcacctgtga tgatggcgca ttttccgtcg agtctcaggt tgtcagaatt
    46801 aaacacataa acctcctgtg aacttcagta aaaataaaag gcaccgcctg tgtgccacat
    46861 tttttatcat taaacttgag gaaaggacac taccaggtat agcagataaa gagaggatta
    46921 tttcgccgca atgagccggg cgggaataat aagattgcgt gaatgcgtct cttcatgggt
    46981 gattttttgc atcatgcgat ccgcaagtgt ggtaccaagt tcccgcgcag gcgtgctggc
    47041 ccaggtaacg ggtatatcat caagtgtggt tggtgtcgca tcggtaaatg ccgccagcga
    47101 aacctgttgc tcaaagtaac gatcgactcc gctttccccg ctttgccgcc ctgctttcag
    47161 caaaccaaac catgccccca tcgcaatagt ttcgttatag caaaccacgg cactgatggt
    47221 cgggttatga cgtaaaagcg ccgtgatagc ttccgcggct tgcttctggc tggaagtgca
    47281 ctccaacacc caatcgctgt gaaacggcag gccaaatttt agtagagttg cacaatagcc
    47341 ccccacccgt tctgcacggg ttaatgagga actttgccct cccagccagg cgatccgctg
    47401 atgcccattg cgaatgagat gctccgtcaa caactgtgca gcctgcatgt tgtccgggcg
    47461 aaccgtatca acatcatcaa gataactggc acgggaagcg aaaatcaccg ggatagcttt
    47521 ttcttctgcc atccgtcgca ggtcatcgct gcttcctgca gccccggcaa ttaccacacc
    47581 atcgacaccc tgattcagta acagtgaaaa ccgctgtgcc agctgctcgc cgtctttacc
    47641 gccgtgaagc aaaaaaacca tccgtccctg cgcttccaga gcttccgtca atccggccgt
    47701 caattcggcg taaaacggcg cagataaatc acggacgatc aaaccaatga cgccgctttg
    47761 cccaccgcgc agcgccgacg cctggcgatt gcgcacaaat cccagctctt caatggcggc
    47821 gttaacgcgt tctcctgtgg cggtagagat tcgccctttg ccactaagca ccagcgaaac
    47881 ggtacttacc gacacgcccg cagccagcgc aacatcatga atggttattt ttttggcggt
    47941 agccatgcag atgacctact ccctgatttt gtgacagata aaacgtttta ccttttattt
    48001 atcttatacc cgctattatc gttgcgtaat gtgatttatg cctcactaaa atttgataaa
    48061 acgttttatc ttctcgcgca atttactgaa tccagattgt tctctacgag gagtcgtttt
    48121 atgacggcga aaacagcacc gaaagtcacg ctgtgggagt tcttccagca gttaggcaaa
    48181 accttcatgt tacccgtggc attattgtcg ttctgcggca ttatgctcgg cattggtagt
    48241 tcccttagta gccacgatgt catcaccctg ctcccggtcc tgggcaaccc cgtgttgcag
    48301 gctatcttta cctggatgag taagattggc tcgtttgctt ttagtttcct gccagtcatg
    48361 ttctgtatcg ccatcccgct gggtctggca cgagaaaaca aaggcgtagc ggcattcgct
    48421 ggcttcgtcg gttatgcggt aatgaacctc gcggtaaact tctggttgac caataaaggc
    48481 attctgccaa ccacggatgc cgccgttctg aaagccaata acatccagag cattcttggg
    48541 atccagtcga tcgacaccgg gatcctcggt gcggtgatcg ccggtattat cgtctggatg
    48601 ctgcatgagc gattccataa tatccgcctg ccggatgcgc tggcattctt cggcggtacg
    48661 cgcttcgtac caatcatctc ctcgctggtg atgggccttg tcggcctggt gattccatta
    48721 gtctggccga ttttcgccat gggtattagc ggcttaggcc atatgatcaa tagcgcgggt
    48781 gatttcggac cgatgctgtt tggtaccggt gaacgtctgc tgttgccgtt tggtctgcat
    48841 cacattctgg tggcattaat tcgctttacc gacgcaggtg gcacgcagga agtctgcggt
    48901 caaaccgtca gcggcgcatt gaccatcttc caggcgcaat tgagttgccc gaccactcac
    48961 ggtttttctg aaagcgccac gcgtttcctt tcgcaaggca aaatgcctgc gtttctcggc
    49021 ggtctgccag gtgcagcgtt agcgatgtat cactgcgcgc gcccggaaaa tcgccataaa
    49081 attaaagggc tgctgatttc tggcctgatc gcctgtgtcg ttggcggcac gaccgaaccg
    49141 ctggaattcc tgttcctgtt cgtagcgcca gttctgtatg tcatccacgc gctgttaacc
    49201 ggcctcggct tcaccgtcat gtctgtgctc ggcgtcacca tcggtaatac cgacggcaat
    49261 atcatcgact tcgtggtgtt cggtattttg catggtctgt caaccaagtg gtacatggtg
    49321 ccagtggtgg tggcaatctg gtttgtcgtt tactacgtca tcttccgttt cgctatcacc
    49381 cgcttcaatc tgaaaacccc ggggcgcgat agcgaagtgg ccagttcaat cgaaaaagcc
    49441 gttgccggtg cgccgggtaa atcaggttac aacgttccgg caatcctcga agccttaggc
    49501 ggtgccgaca atattgttag cctcgataac tgcattaccc gtctgcgttt gtctgtgaaa
    49561 gatatgtcgc ttgttaatgt gcaggcactg aaggacaatc gggcaattgg cgtagtacaa
    49621 cttaatcaac ataacctgca ggttgttatc gggccacaag ttcagtcagt aaaagatgaa
    49681 atggccggtc tgatgcatac tgtccaggca taaggataag atatgttcga tttttcaaag
    49741 gtcgtggatc gtcatggcac atggtgtacg cagtgggatt atgtcgctga ccgtttcggc
    49801 actgctgacc tgttaccttt cacgatttca gacatggatt ttgccactgc cccctgcatt
    49861 atcgaggcgc tgaatcagcg cctgatgcac ggcgtgtttg gctacagccg ctggaagaac
    49921 gatgagtttc tcgcggctat tgcccactgg ttttccaccc agcattacac cgccatcgat
    49981 cctcagtcgg tggtgtatgg cccttctgtt atctatatgg tctcagaact gattcgtcag
    50041 tggtctgaaa caggtgaagg cgtggtgatc cacacacccg cctatgacgc attttacaag
    50101 gccattgaag gtaatcagcg cacagtaatg cccgttgctt tagagaagca ggctgacggc
    50161 tggttttgcg atatgggcaa gctggaagcc gtgttggcga aaccagaatg taaaattatg
    50221 ctcctgtgta gcccacagaa tcctaccgga aaagtgtgga cgtgcgatga gctggagatc
    50281 atggctgacc tgtgcgagcg tcatggtgtg cgggttattt ccgatgaaat ccatatggat
    50341 atggtttggg gcgagcagcc gcatattccc tggagtaatg tggcgcgggg agactgggcg
    50401 ttgctgacgt cgggatcgaa aagtttcaat attcccgcgc tgaccggtgc ttacgggatt
    50461 atagaaaata gcagcagccg tgatgcctat ttatcggcac tgaaaggacg tgatgggctt
    50521 tcttcccctt cggtactggc gttaactgcc catatcgccg cctatcagca aggtgcaccg
    50581 tggctggatg ccttacgcgt ctatctgaaa gataacctga cgtatatcgc agataaaatg
    50641 aacgccgcgt ttcctgaact caactggcag atcccgcaat ccacttatct ggcctggctt
    50701 gatttacgtc cgttgaatat tgacgacaac gcgttgcaaa aagcacttat cgaacaagaa
    50761 aaagtcgcga tcatgccggg gtatacctac ggtgaagaag gtcgtggttt tgtccgactc
    50821 aatgccggct gcccacgttc gaaactggaa aaaggtgtgg ctggattaat taacgccatc
    50881 cgcgctgttc gctaacccca attgcgcaac gtaaaaaatc gttgcgcaat cgtggatttt
    50941 taccctgctt tgtttttata atggtgcgca cttttatatc cagaaaaaga gtgcgaccat
    51001 gattgatacc accctgccat taactgatat ccatcgccac cttgatggca acattcgtcc
    51061 ccagaccatt cttgaacttg gccgccagta taatatctcg cttcctgcac aatccctgga
    51121 aacactgatt ccccacgttc aggtcattgc caacgaaccc gatctggtga gctttctgac
    51181 caaacttgac tggggcgtta aagttctcgc ctctcttgat gcttgtcgcc gcgtggcatt
    51241 tgaaaacatt gaagatgcag cccgtaacgg cctgcactat gtcgagctgc gtttttcacc
    51301 aggctacatg gcaatggcac atcagctgcc tgttgcaggt gttgttgaag cggtgatcga
    51361 tggcgtacgt gaaggttgcc gcacctttgg tgtgcaggcg aagcttatcg gcattatgag
    51421 ccgaaccttc ggcgaagccg cctgtcagca agagctggag gcctttttag cccaccgtga
    51481 ccagattacc gcacttgatt tagccggtga tgaacttggt ttcccgggaa gtctgtttct
    51541 ttctcacttc aaccgcgcgc gtgatgcggg ctggcatatt accgtccatg caggcgaagc
    51601 tgccgggccg gaaagcatct ggcaggcgat tcgtgaactg ggggcagagc gcattggaca
    51661 tggcgtaaaa gccattgaag atcgggcgct gatggatttt ctcgccgagc aacaaattgg
    51721 tattgaatcc tgtctgacct ccaatattca gaccagcacc gtggcagagc tggctgcaca
    51781 tccgctgaaa acgttccttg agcatggcat tcgtgccagc attaacactg acgatcccgg
    51841 cgtacaggga gtggatatca ttcacgaata taccgttgcc gcgccagctg ctgggttatc
    51901 ccgcgagcaa atccgccagg cacagattaa tggtctggaa atggctttcc tcaacgcaga
    51961 ggaaaaacgc gcactgcgag aaaaagtcgc agcgaagtaa caaaaatgga tggtgcaaat
    52021 tgcaccatcc atttttcatg caaggcacaa agtcgcgcga tgtttggcgg attcgatgcc
    52081 cagctcaatc aactccatta cctggattgc ctggcttgcc ggaaccggat tttcaccatc
    52141 gccatttaac gcatcacgaa tcgccgcata gtaagccgga taattcccag gcaccgtcaa
    52201 cagcgtttct tcgacacgtt cctcaccttc cacgcgggta agtacgccat cacgcatatc
    52261 gtagccccag tcttcctgcg gcagacgctc gccatttttc agacgttctt cctgtggatc
    52321 gaggccatat ttcacataac tgcctcggga tccatgcacg atataccgtg ctgactcagc
    52381 ggctgccagc atggtaccgt gtaaaatgac tcgccgctgt ggataggaca agatggcgtg
    52441 gaaataatcg gtcgactgcg ctccgggccg taactgtgcc aaatctaccg tcatgctgac
    52501 cggtaaacca aatagcgtaa tggcctgatc aagaagatgt ggtgctaaat cgtaccagat
    52561 accgctgcct gggccgccct gttcacgcca acgatcgcgc acctgcggac ggaagcggtc
    52621 aaaatgagac tcaaagtaag caacttcacc cagcacgcct tccgcgagca aaccttttag
    52681 cgtcaagaaa tcgctatccc aacgacggtt atggaataca gacagaacac gccccaggct
    52741 ttttgccagc gcatccagct ctcgcgcttg tgacagtgtc acggtaaagg gtttatcaac
    52801 gaccacatgt ttacccgcct caagcgctgc tttggctaac gggaaatggg tatcgttggg
    52861 tgtaggaatg acaatcaggt ctatgttggg atcgttaaac agatgcttcg gctcagagac
    52921 aaccgtaacc gttggccagt cggcttttac ttttgtttca tcactgctgg agattaccgc
    52981 cagttccagc ccgggtgtgc ccgcaatcag gggcgcatgg aaggttttgc tcgcataacc
    53041 atacccaatc aacccaacac ggatgttgtc gctcatgtca ttgcctctca ttgaagtatg
    53101 atggctattt gacactatcc tttaccctcg ctcaacagtt taataacctg ccagcaataa
    53161 gggatgttgt ttaacttaag tcaaaaaaat agcgattttt ccaacgacaa aagctaaata
    53221 tcgcaaaaac ctcagtaaaa atcttgctgg agctattatt gctaagtaac atttaccccc
    53281 tgaagttaat ggatcaatca acagagatgt gagttgtaat gaatcgtctt attgaattaa
    53341 caggttggat cgttcttgtc gtttcagtca ttcttcttgg cgtggcgagt cacattgaca
    53401 actatcagcc acctgaacag agtactatgg tacaacacaa gtaagccctg cgcttgtaga
    53461 gcgacatgct gcccgtcagg gtgcatgttt tcacttgtcg gatattaaac caggaattta
    53521 ttatcttgtt cggtgttgtt ggtgattgtc agggatagta aagcgcgtta ctcttttcat
    53581 agcagttttt catccctgaa tatttgccat cagcgcattc ttattattgt aataacacaa
    53641 gcgttcactt ttggtgaaat tttatcatca ctaataatac ctaccccttt ggtattaatt
    53701 attttatgga ccttttatga ctgttcagga ctacttatta aaatttcgca aaatcagttc
    53761 actcgaaagt ctggaaaaac tctacgacca tcttaattac accctgacgg acgatcagga
    53821 actgatcaat atgtatcgtg ctgccgatca ccgtcgcgca gagctggttt ctggcgggcg
    53881 tttgtttgac ctcggcctgg taccgaagtc cgtctggcac tatgttcaat aaagaaagta
    53941 gcgatcggtt ataaaagcct tataataacg cccctaaaat tagatttgcc tgtttgcgtc
    54001 gctattctgg agaacctatg accaccacaa cgccacaacg tattggaggc tggttgcttg
    54061 gcccgttggc ctggctttta gtcgctctgt taagtacgac gctggcgttg ttgctgtata
    54121 ccgctgcgtt atcttctccg caaacatttc aaacgctcgg cgcacaagca ctgacgacac
    54181 aaatcttatg gggcgtttct tttattaccg ctatcgccat gtggtattac acactgtggt
    54241 tgaccatcgc tttctttaaa cgtcggcgtt gtgttcctaa acactatatt atctggctgc
    54301 ttatttccgt attgctggcg gtaaaagcgt tcgctttttc acccgtggaa gatggcatag
    54361 ccgttcgcca gttgctgttt actttgttgg caaccgcatt gatcgtcccc tatttcaaac
    54421 gctcgtcgcg ggtgaaagcg acgtttgtga atccgtaata accttacagt taacctgttg
    54481 tcgcctgctc tggattaacg gataataggc ggctttttta tttcaggccg aaaaatgact
    54541 gactacctgt tactgtttgt cggaactgta ctggtcaata actttgtact ggtcaagttt
    54601 ctcggtctct gtccgtttat gggggtttcc aaaaagctgg aaaccgcgat gggcatgggg
    54661 ctggcaacaa cgtttgtgat gacgctggcg tctatttgcg cctggcttat cgatacgtgg
    54721 attttgatcc cacttaatct gatttacctg cgcaccctgg catttattct ggtgattgct
    54781 gtggtcgtgc agttcaccga gatggtggtg cgcaaaacca gcccggtgct ttaccgtttg
    54841 ctggggattt ttttgccgct tatcaccacc aactgtgccg tgctcggcgt ggcgttgctg
    54901 aatatcaatc tcgggcacaa tttcttgcag tcggcgctgt acggtttttc cgccgctgtc
    54961 ggtttttcgc tggtgatggt tctcttcgca gccatccgcg aacgccttgc tgtggctgat
    55021 gtcccggcac cttttcgcgg taatgccatt gcgttaatta ccgcaggtct tatgtctctg
    55081 gcctttatgg gctttagtgg tttggtgaag ttgtaatgaa tgctatctgg attgccgttg
    55141 ccgccgtgag cctgctgggc ctggcgtttg gcgccattct gggttatgcc tcccgccgtt
    55201 ttgcggtgga agacgatccg gtcgttgaga aaattgacga aatcttaccg cagagccagt
    55261 gtggtcagtg cggttatccc ggctgtcgcc cctacgcgga agccatcagc tgtaacggtg
    55321 aaaaaatcaa ccgttgcgcc cctggtggcg aagctgtgat gctaaaaatt gccgagttgc
    55381 ttaatgtcga gccgcagccg ctggatggcg aagcgcaaga gctaacgcct gcgcggatgg
    55441 tggcggttat tgatgaaaat aactgtattg gctgcactaa atgtattcag gcgtgtccgg
    55501 tagacgccat cgttggcgct acccgtgcca tgcatacggt aatgagtgat ctctgtacgg
    55561 gctgcaattt atgtgttgat ccgtgcccga cgcactgcat ctcgttgcaa ccggtcgcag
    55621 aaacacctga ctcctggaaa tgggatctga acaccattcc cgtgcgtatc attcccgtgg
    55681 aacaccatgc ttaagttatt ctctgcattc agaaaaaata aaatctggga tttcaacggc
    55741 ggcatccatc caccggagat gaaaacccag tccaacggta cacccctgcg ccaggtacca
    55801 ctggcgcagc gttttgtcat tccactgaaa cagcatattg gcgctgaagg tgagttgtgc
    55861 gttagcgtcg gcgataaagt attgcgcggc cagccgctta cccgtggtcg cggcaaaatg
    55921 ctgcctgttc acgcgcccac ctcgggtacc gttacggcta ttgcgcccca ctctacggct
    55981 catccttcag ctttagctga attaagcgtg attattgatg ccgatggtga agactgctgg
    56041 atcccacgag acggctgggc cgattatcgc tctcgccgtc gcgaagagtt aatcgagcgc
    56101 attcatcagt ttggtgttgc cgggctgggt ggtgcaggat tcccgacagg cgttaaattg
    56161 cagggcggcg gagataagat tgagacgttg attatcaacg ctgctgagtg cgagccatac
    56221 attaccgccg atgaccgttt gatgcaggat tgcgcggctc aggtcgtaga gggtattcgc
    56281 attcttgcgc atattctgca gccacgcgaa attcttatcg gcattgaaga taacaaaccg
    56341 caggcgattt ccatgctgcg cgcggtgctg gcggactctc acgatatttc tatgcgggtg
    56401 attccaacca aatatccttc tggcggtgct aaacaattaa cctacattct gaccgggaag
    56461 caggttccac atggcgggcg ttcatccgat atcggcgtat taatgcaaaa cgtcggcact
    56521 gcttatgcag tgaaacgtgc cgttattgat ggcgagccga ttaccgagcg tgtcgtaacc
    56581 ctgactggcg aagcaatcgc tcgcccgggc aacgtctggg cacggctggg gacgccagtg
    56641 cgtcatttat tgaatgacgc cggattctgc ccctctgccg atcaaatggt gattatgggc
    56701 ggcccgctaa tgggctttac cttgccatgg ctggatgtcc cggtcgtaaa gattaccaac
    56761 tgtctgttgg ctccctctgc caatgagctt ggcgaaccac aggaagaaca aagctgcatc
    56821 cggtgtagcg cctgtgctga cgcctgccct gcggatcttt tgccgcaaca gttgtactgg
    56881 ttcagcaaag gtcagcaaca cgataaagct accacgcata acattgccga ctgcattgaa
    56941 tgtggggctt gcgcgtgggt ttgcccgagc aatattcccc tggtgcaata tttccgtcag
    57001 gaaaaagctg aaattgcggc tattcgtcag gaagaaaagc gcgccgcaga agccaaagcg
    57061 cgtttcgaag cgcgcctggc tcgtctggag cgcgaaaaag cggctcgcct tgaacgacat
    57121 aagagcgcag ccgttcaacc tgcagccaaa gataaagatg cgattgctgc cgctctggcg
    57181 cgggtgaaag agaaacaggc ccaggctaca cagcctattg tgattaaagc gggcgaacgc
    57241 ccggataaca gtgcaattat tgcagcacgg gaagcccgta aagcgcaagc cagagcgaaa
    57301 caggcagaac tgcagcaaac taacgacgca gcaaccgtta ctgatccacg taaaactgcc
    57361 gttgaagcag ctatcgcccg cgccaaagcg cgcaagctgg aacagcaaca ggcaaatgcg
    57421 gaaccagaac aacaggtcga tccgcgcaaa gctgccgtcg aagccgctat tgcccgtgcc
    57481 aaagcacgca agctggaaca gcaacaggct aatgcggaac cagaacaaca ggtcgatccg
    57541 cgcaaagccg ccgtcgaagc cgctatcgcc cgcgccaaag cgcgcaagct ggaacagcaa
    57601 caggctaatg cggaaccaga agaaccggtc gatccgcgca aa
//
LOCUS       DAZKIF010000010        56049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZKIF010000010 DAZKIF010000000
VERSION     DAZKIF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.010"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            118..2259
                     /locus_tag="ACHOTW_000700"
     CDS             118..2259
                     /locus_tag="ACHOTW_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFP9842865.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHCVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2323..4215)
                     /gene="parE"
                     /locus_tag="ACHOTW_000701"
     CDS             complement(2323..4215)
                     /gene="parE"
                     /locus_tag="ACHOTW_000701"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9842866.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4244..4825)
                     /gene="yqiA"
                     /locus_tag="ACHOTW_000702"
     CDS             complement(4244..4825)
                     /gene="yqiA"
                     /locus_tag="ACHOTW_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP9842867.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4825..5652)
                     /gene="cpdA"
                     /locus_tag="ACHOTW_000703"
     CDS             complement(4825..5652)
                     /gene="cpdA"
                     /locus_tag="ACHOTW_000703"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP9842868.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5677..6099)
                     /gene="yqiB"
                     /locus_tag="ACHOTW_000704"
     CDS             complement(5677..6099)
                     /gene="yqiB"
                     /locus_tag="ACHOTW_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP9842869.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6100..6729)
                     /gene="nudF"
                     /locus_tag="ACHOTW_000705"
     CDS             complement(6100..6729)
                     /gene="nudF"
                     /locus_tag="ACHOTW_000705"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP9842870.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6934..8415
                     /gene="tolC"
                     /locus_tag="ACHOTW_000706"
     CDS             6934..8415
                     /gene="tolC"
                     /locus_tag="ACHOTW_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP9842871.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8563..9234
                     /gene="ygiB"
                     /locus_tag="ACHOTW_000707"
     CDS             8563..9234
                     /gene="ygiB"
                     /locus_tag="ACHOTW_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP9842872.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9240..10400
                     /gene="ygiC"
                     /locus_tag="ACHOTW_000708"
     CDS             9240..10400
                     /gene="ygiC"
                     /locus_tag="ACHOTW_000708"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9842873.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(10438..11226)
                     /gene="ygiD"
                     /locus_tag="ACHOTW_000709"
     CDS             complement(10438..11226)
                     /gene="ygiD"
                     /locus_tag="ACHOTW_000709"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP9842874.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            11369..12142
                     /gene="zupT"
                     /locus_tag="ACHOTW_000710"
     CDS             11369..12142
                     /gene="zupT"
                     /locus_tag="ACHOTW_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP9842875.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(12200..12370)
                     /gene="yqiD"
                     /locus_tag="ACHOTW_000711"
     CDS             complement(12200..12370)
                     /gene="yqiD"
                     /locus_tag="ACHOTW_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP9842876.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(12632..13285)
                     /gene="ribB"
                     /locus_tag="ACHOTW_000712"
     CDS             complement(12632..13285)
                     /gene="ribB"
                     /locus_tag="ACHOTW_000712"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP9842877.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(13389..13537)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            13659..13958
                     /gene="ubiK"
                     /locus_tag="ACHOTW_000713"
     CDS             13659..13958
                     /gene="ubiK"
                     /locus_tag="ACHOTW_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP9842878.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(13993..14193)
                     /gene="glgS"
                     /locus_tag="ACHOTW_000714"
     CDS             complement(13993..14193)
                     /gene="glgS"
                     /locus_tag="ACHOTW_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP9842879.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            14462..15076
                     /gene="yqiJ"
                     /locus_tag="ACHOTW_000715"
     CDS             14462..15076
                     /gene="yqiJ"
                     /locus_tag="ACHOTW_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP9842880.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            15118..16779
                     /gene="yqiK"
                     /locus_tag="ACHOTW_000716"
     CDS             15118..16779
                     /gene="yqiK"
                     /locus_tag="ACHOTW_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9842881.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            17018..17077
                     /locus_tag="ACHOTW_000717"
     CDS             17018..17077
                     /locus_tag="ACHOTW_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9842882.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            17394..17453
                     /gene="ibsE"
                     /locus_tag="ACHOTW_000718"
     CDS             17394..17453
                     /gene="ibsE"
                     /locus_tag="ACHOTW_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFP9842883.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(17573..19006)
                     /gene="hldE"
                     /locus_tag="ACHOTW_000719"
     CDS             complement(17573..19006)
                     /gene="hldE"
                     /locus_tag="ACHOTW_000719"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP9842884.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(19054..21894)
                     /gene="glnE"
                     /locus_tag="ACHOTW_000720"
     CDS             complement(19054..21894)
                     /gene="glnE"
                     /locus_tag="ACHOTW_000720"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP9842885.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRWELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(21917..23218)
                     /gene="ygiF"
                     /locus_tag="ACHOTW_000721"
     CDS             complement(21917..23218)
                     /gene="ygiF"
                     /locus_tag="ACHOTW_000721"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP9842886.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            23460..24080
                     /gene="ygiM"
                     /locus_tag="ACHOTW_000722"
     CDS             23460..24080
                     /gene="ygiM"
                     /locus_tag="ACHOTW_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP9842887.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            24144..25382
                     /gene="cca"
                     /locus_tag="ACHOTW_000723"
     CDS             24144..25382
                     /gene="cca"
                     /locus_tag="ACHOTW_000723"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP9842888.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(25563..26384)
                     /gene="bacA"
                     /locus_tag="ACHOTW_000724"
     CDS             complement(25563..26384)
                     /gene="bacA"
                     /locus_tag="ACHOTW_000724"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9842889.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(26475..26843)
                     /gene="folB"
                     /locus_tag="ACHOTW_000725"
     CDS             complement(26475..26843)
                     /gene="folB"
                     /locus_tag="ACHOTW_000725"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP9842890.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            26949..27566
                     /gene="plsY"
                     /locus_tag="ACHOTW_000726"
     CDS             26949..27566
                     /gene="plsY"
                     /locus_tag="ACHOTW_000726"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9842891.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(27579..28511)
                     /gene="ttdR"
                     /locus_tag="ACHOTW_000727"
                     /gene_synonym="dan"
     CDS             complement(27579..28511)
                     /gene="ttdR"
                     /locus_tag="ACHOTW_000727"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP9842892.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            28718..29629
                     /gene="ttdA"
                     /locus_tag="ACHOTW_000728"
     CDS             28718..29629
                     /gene="ttdA"
                     /locus_tag="ACHOTW_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP9842893.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            29626..30231
                     /gene="ttdB"
                     /locus_tag="ACHOTW_000729"
     CDS             29626..30231
                     /gene="ttdB"
                     /locus_tag="ACHOTW_000729"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP9842894.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            30280..31743
                     /gene="ttdT"
                     /locus_tag="ACHOTW_000730"
     CDS             30280..31743
                     /gene="ttdT"
                     /locus_tag="ACHOTW_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP9842895.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(31786..32799)
                     /gene="tsaD"
                     /locus_tag="ACHOTW_000731"
     CDS             complement(31786..32799)
                     /gene="tsaD"
                     /locus_tag="ACHOTW_000731"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9842896.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDLTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            33037..33252
                     /gene="rpsU"
                     /locus_tag="ACHOTW_000732"
     CDS             33037..33252
                     /gene="rpsU"
                     /locus_tag="ACHOTW_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9842897.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            33363..35108
                     /gene="dnaG"
                     /locus_tag="ACHOTW_000733"
     CDS             33363..35108
                     /gene="dnaG"
                     /locus_tag="ACHOTW_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9842898.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            35303..37144
                     /gene="rpoD"
                     /locus_tag="ACHOTW_000734"
     CDS             35303..37144
                     /gene="rpoD"
                     /locus_tag="ACHOTW_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9842899.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(37223..37729)
                     /gene="mug"
                     /locus_tag="ACHOTW_000735"
     CDS             complement(37223..37729)
                     /gene="mug"
                     /locus_tag="ACHOTW_000735"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP9842900.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            37854..37929
                     /locus_tag="ACHOTW_000736"
     tRNA            37854..37929
                     /locus_tag="ACHOTW_000736"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37887..37889,aa:Ile,seq:cat)
     gene            complement(37983..38747)
                     /gene="nfeF"
                     /locus_tag="ACHOTW_000737"
                     /gene_synonym="yqjH"
     CDS             complement(37983..38747)
                     /gene="nfeF"
                     /locus_tag="ACHOTW_000737"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP9842901.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            39035..39658
                     /gene="nfeR"
                     /locus_tag="ACHOTW_000738"
                     /gene_synonym="yqjI"
     CDS             39035..39658
                     /gene="nfeR"
                     /locus_tag="ACHOTW_000738"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP9842902.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(39812..41332)
                     /gene="aer"
                     /locus_tag="ACHOTW_000739"
     CDS             complement(39812..41332)
                     /gene="aer"
                     /locus_tag="ACHOTW_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP9842903.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            41750..43129
                     /gene="ygjG"
                     /locus_tag="ACHOTW_000740"
     CDS             41750..43129
                     /gene="ygjG"
                     /locus_tag="ACHOTW_000740"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP9842904.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(43171..43503)
                     /gene="ygjH"
                     /locus_tag="ACHOTW_000741"
     CDS             complement(43171..43503)
                     /gene="ygjH"
                     /locus_tag="ACHOTW_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP9842905.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            43722..44705
                     /gene="ebgR"
                     /locus_tag="ACHOTW_000742"
     CDS             43722..44705
                     /gene="ebgR"
                     /locus_tag="ACHOTW_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP9842906.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            44889..47981
                     /gene="ebgA"
                     /locus_tag="ACHOTW_000743"
     CDS             44889..47981
                     /gene="ebgA"
                     /locus_tag="ACHOTW_000743"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP9842907.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            47978..48427
                     /gene="ebgC"
                     /locus_tag="ACHOTW_000744"
     CDS             47978..48427
                     /gene="ebgC"
                     /locus_tag="ACHOTW_000744"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP9842908.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            48490..49923
                     /gene="ygjI"
                     /locus_tag="ACHOTW_000745"
     CDS             48490..49923
                     /gene="ygjI"
                     /locus_tag="ACHOTW_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9842909.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            50057..51127
                     /gene="ygjJ"
                     /locus_tag="ACHOTW_000746"
     CDS             50057..51127
                     /gene="ygjJ"
                     /locus_tag="ACHOTW_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP9842910.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            51144..53495
                     /gene="ygjK"
                     /locus_tag="ACHOTW_000747"
     CDS             51144..53495
                     /gene="ygjK"
                     /locus_tag="ACHOTW_000747"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9842911.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            54021..56039
                     /gene="fadH"
                     /locus_tag="ACHOTW_000748"
     CDS             54021..56039
                     /gene="fadH"
                     /locus_tag="ACHOTW_000748"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP9842912.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT        13719 a        15076 c        14249 g        13005 t
ORIGIN      
        1 ttgaatgatt ctcaattaca tatccgatgg taaaataaca aacggatagt gcttagtgag
       61 caacaagaaa caattttcac tcacgacaat ccgtgagttt ttgggataac agactaaatg
      121 gctaagttca caccttcatt ctcaggaatc aaaggtcggg cgctcttttc actgctcttt
      181 gcagcaccaa tgattcatgc aaccgacact gcaacgacca aagatggcga aacaatcact
      241 gttacagcgg atgcaaatac cgcaactgag gcgaccgatg gttatcaacc tctgagcact
      301 tccacggcga cattaaccga tatgccgatg ctggatatcc cgcaggtggt caatacggtt
      361 agcgatcagg ttctggaaaa tcagaatgca acgacgctgg atgaagcgct ttataacgtc
      421 agtaacgtgg tacagaccaa tacattaggc ggaactcagg acgcctttgt acgtcgtggt
      481 tttggtgcta accgggatgg ctccatcatg accaacggcc tgcgaactgt acttcctcgc
      541 agtttcaacg ccgccacaga acgtgtggaa gtgctaaaag gtccggcctc cacgctgtat
      601 ggcattctcg atcctggtgg attgattaac gtcgtgacca agcgcccgga aaaaacattc
      661 catggttctg tctcagccac gtcctccagt tttggcggag gcactgggca acttgatatc
      721 acaggtccca ttgaaggcac tcagctggca taccgcctga cgggggaagt gcaggatgaa
      781 gattactggc gaaacttcgg taaagagcgc agtacattta ttgccccgtc actcacctgg
      841 tttggtgata atgcaacagt aaccatgctc tattcccatc gggactataa aactccgttc
      901 gatcgtggaa cgattttcga ccttacgacg aaacagcccg taaacgttga tcgaaaaata
      961 cgttttgacg aaccgtttaa tattacagat ggtcagtccg atctggcgca actcaacgca
     1021 gaatatcatc tcaatagcca gtggacagcg cgctttgatt acagctacag ccaggataaa
     1081 tacagcgaca atcaggctcg cgttaccgcg tatgatgcaa cgacaggaac gctgacacgg
     1141 cgtgttgatg caactcaggg atctacccag cgtatgcatt ctactcgtgc ggatctgcaa
     1201 gggaatgttg atattgctgg gttctataat gagattctgg gtggggtgtc atatgaatat
     1261 tatgatcttc tgcgcacaga tatgattcgc tgtaaaaacg ctaaagattt caatatctac
     1321 aaccccgttt atggcaatac cagcaaatgc acaacggttt cggcgtcgga cagtgatcag
     1381 acgatcaaac aggagagcta ctcagcttat gcacaggatg cgctctatct gaccgataac
     1441 tggattgccg tcgccgggat ccgctatcag tattacacgc agtatgcggg taaaggccgt
     1501 ccttttaatg tcaatactga cagccgcgat gaacaatgga cgcccaaact ggggttagtc
     1561 tacaaactga cgccatcggt atccttattt gctaattatt cgcaaacatt tatgccgcaa
     1621 tcgtcaattg ccagctacat cggagatctt ccaccggaat catctaatgc ttacgaagtc
     1681 ggggcaaaat tcgagctgtt cgatggtata accgcagata ttgcgctgtt tgatatccat
     1741 aaacgtaacg tgttgtatac cgaaagtatt ggtgatgaaa ccatcgccaa aacggcaggc
     1801 cgcgttcgtt caagaggggt agaagtcgac cttgcgggag cattaactga aaacattaat
     1861 atcattgcca gctacggcta taccgatgct aaggttctgg aagatcctga ttatgcaggg
     1921 aaaccattgc cgaatgttcc tcgtcatacc ggttcgctat tcctgaccta tgacattcat
     1981 aacatgccag gcaataacac actgacgttt ggcggtggtg gacattgcgt aagccgtcgt
     2041 tcggcaacca atggggctga ctattatctg ccaggctatt tcgttgccga tgccttcgcc
     2101 gcatacaaaa tgaaattgca gtatccggtc acactgcaat taaacgtcaa aaacctgttt
     2161 gataaaacgt attacacctc ttccatcgcc acaaataatc tggggaatca gattggcgat
     2221 ccgcgtgaag tgcaattcac ggtgaaaatg gaattttgat caaaaaagcc cggtatacac
     2281 ttgtataccg ggctatgttt tattcttctg gcgggaggat tatcagacct caatctccgc
     2341 catgtcgcct ttctcttgca accagttgcg gcgatcttcc gagcgtttct tcgccagcag
     2401 catatccatc atcgcgtcag tacgctgatc gtcttcatca tcgatagtca gctgcaccag
     2461 acggcgagtg ttcggatcaa gcgtggtttc gcgcaattgc atcgggttca tttcccccag
     2521 acctttaaaa cgctggacgt tcggcttgcc tttcttgcgt tttaattgct caagtacgcc
     2581 ctctttctct tcttccgtca gcgcgtaata aacctctttc ccgagatcaa tacggtagag
     2641 cggtggcagt gcgacgtaaa cgtgaccgtg tttcaccaac gcgcggaagt gttttacgaa
     2701 caaagcgcag agcagtgtag caatgtgcaa accatcagag tccgcatccg cgaggataca
     2761 gattttgcca taacgaagct ggctcagatc gtcgctgtca ggatcgatac cgatcgctac
     2821 cgaaatatca tgcacttcct gcgaggccag cacttcgtcg gaagaaactt cccaggtgtt
     2881 aaggatctta cctttcagcg gcatgatcgc ctgatattcg cgatcgcgcg cctgcttggc
     2941 agatccgcct gcggagtcac cttccacgag gaacagttcg gtacggttaa ggtcctgcgc
     3001 ggtacaatca gccagtttgc caggcagcgc cgggccgctg gtcagctttt tacgcaccac
     3061 ttttttagcc gcacgcatac gacgctgggc gctggaaatc gccatctctg ccagcaactc
     3121 cgccgcctga acgttctggt tcagccacag gatgaaggca tctttcacca cgcccgaaac
     3181 gaatgccgca cactgacgcg aagagagacg ctctttggtc tgcccggcaa actgtggatc
     3241 ctgcattttt actgacagca cataggcgca gcgatcccag atatcttccg ccgacaactt
     3301 tacaccgcgc ggcaaaatgt tgcggtattc acagaactca cgcatcgcgt ccagcaggcc
     3361 ctgacgcagg ccattaacat gggtaccgcc ctgcatcgtt gggatcaggt tgacgtagct
     3421 ttcggtcagc agttcaccgc cttccggcag ccacagtagc gcccagtcca ccgcttcagt
     3481 atcgccagcg aaattaccga taaacggttt ttctggcagc gtcggtaaac cgtttaccgc
     3541 ttccgccagg taatcattca gaccgtcctg atagcaccag cgctgttcgg tgttgttgat
     3601 ctcatcttta aaggtaatct caacgccggg gcacagtact gctttggctt tcagcacatg
     3661 cgtcaggcgt gaaacagaaa aacgcgggct atcgaagaag gtttcatccg gccagaaatg
     3721 gacgctggtg ccggtattgc gtttaccgca attgccgaca acctgtaaat cctgcacctt
     3781 ttcaccattt tcaaaggcga tgttataaac ctgaccatcg cggcgcacgt taacttctac
     3841 gcgcttcgac agggcgttaa ccaccgaaat ccccacgcca tgcaggccgc cagagaactg
     3901 gtaatttttg ttagagaatt taccgcctgc atgcagacgg caaagaatca gttcaaccgc
     3961 cggtacaccc tcttccgggt gaatatccac cggcatcccg cgcccatcgt caataacttc
     4021 taacgactgg tcagcatgta aaataacgtc cacgcgtttt gcgtgacccg ccagtgcttc
     4081 atccacactg ttatcaatga cttcttgccc caaatggtta gggcgagtgg tatcggtata
     4141 catccccgga cggcggcgaa ccggctcaag cccggtgagt acctcaatgg catcagcgtt
     4201 ataagtttgc gtcatggttt aagttagcaa ttcgagttga tcgtcagaga tggtgcagac
     4261 caagaaaatc gacgatcggg ttgaaataat cttcgaagcc cgtgaatgcg tggttgccgc
     4321 cttctatgac agtctggcgg caggaagcgt agtacgccac cgcctggcgg taatccagca
     4381 cttcatctcc cgtctgttgc agcagccaga tcaaatccgg cgcttccagc gggtcaatct
     4441 gcatgacttt aagatcgtaa atatggcgtg actctagcac atattgctgc ccggtgtagg
     4501 ggttctcgtt ctgaccgaga tagtccgtca gcagttcaaa cgggcgcacc gccgggttta
     4561 ccaccactgc gggcagcata aaacattgtg acaaccaggt ggcgtaatat ccccccagtg
     4621 acgaaccgac aatacccagc gaatcgccgc catgttccag gacaatggat tccagcagct
     4681 ctgccgcgtc ggaaggatac ggcggcaact gcggaatgat catctcaacg tcagggtgat
     4741 gttccgccag ccagtttttt aacaagctcg cttttgcaga gcgcggcgag ctgttgaaac
     4801 cgtgtaaata aagaagcgta gacatcagta gccttctgaa gcggtatcag gttggaaacg
     4861 tgtgtccgcc aggcgatgca cctcggtggt cagcgtgcca tcagcatgta actcgagagt
     4921 acgccagccg ggcgcgatag tatccagcgt aaagttggaa cagtgcggct taaactgcac
     4981 acaggtcgac ggtgttgcca gcaggcggcg accgttccag tcgagatcca gctcctgatg
     5041 aatatgaccg cacagcaagt atttgacgtg cggaaacttc gccagcacgg tatccagttc
     5101 gcccgcgtta cgcaggctgt gttgatcaag ccaactacaa cccgcaggta gcggatgatg
     5161 atgcagcagc agcaacgtat ggcgctctgg cgcatcagcc agtttacgtt ccagccactc
     5221 aagctgaaac tcgcttagct caccgtgcgg cacgccaaac acctggctat ccagcaacag
     5281 gatttgccat tgctcaccaa taaacacccg cttcgccggg gagatacccg catcctgtaa
     5341 cgcgctgtac atcgcgggct ggaaatcgtg gttgcccggc agccagacgc agggcgcacg
     5401 aaaacttgcg atgccttcag cgaaatgctg ataggccgca gaggattgat cctgcgctaa
     5461 atcacctgtc gcgacaatca ggtcgaattc gtgctggtgt ggacgaatcg cctccagcac
     5521 cgcctggtag ctttcccagg tgtttacccc taagagggct tcgtgctttt gtgcaaacag
     5581 gtgagtatcg gtaatttgta aaatcctgac tctggcctca ccagccagag gaagggttaa
     5641 caggctttcc aaatggtgtc cttaggtttc acgacgctaa taaaccggaa tcgccatcgc
     5701 tccatgtgct aaacagtatc gcaaccagtc cgctaaaaac tggttaattt gatgcttttc
     5761 gtcgcgttga tgcaactttt tattaggata atcataccgc gctttgaagc gaaaaatctg
     5821 ctggcttgaa cacacttcag ccaccatcgc gtcatgatac agacgcaccg tcattgacgg
     5881 aaggctccag taactgatcg cgggcgcagt ctgttctatt gtcaccaggg tagtgtatcg
     5941 ggtcgattcc acaatcgtca gccgatattg tgcgtttgcc acctgatagc ttacagtttc
     6001 gccgggtgcg tcattgcgcg gtaacaaacg gcgcaattgt gaaaagttca tctcgcacag
     6061 gcgcatcatt tcaggaaagt caggtgtgta acgcttcatt tatgcccact cattttttaa
     6121 cgcttgatga tgcagctgca gccattgcaa agcgatgacc gacgctgcgt tgtcgatttt
     6181 cccctcttct acccactggt atgcctgttc ccggcttacc acatgaacgc gaatatcttc
     6241 gttttcatca gccagaccgt gaataccgct tgcggtcgtg gcgtccactt cgcccaccat
     6301 aattgacgaa cgctcactgg tgccccccgg gcttgccagg aaacttaaca ccggtttggt
     6361 ccgtttgact atcagccccg cctcttcaat cgcttcgcga cgggcaacat cttccacact
     6421 ttcaccctct tcaatcattc cggcaaccat ctccagtagc caaggggttt cgctagtgtc
     6481 gtatgcggca attcgaatct gctcaatcag cacaacttca tcacgcactg ggtcaaaggg
     6541 tagcaagact gcggcgtgac cgcgctcaaa aatttcccgc cgtacctcat gactcatttg
     6601 cccgttgaat agacgatgac gaaatctata aagatctaat gaaaaaaagc cgcgataaag
     6661 tgtttctcgt gcaataattt ctacatcgtt tttgccaaat gtaacgggca ggttgtctgg
     6721 cttaagcatt gttaatgtcc tggcactaat agtgaattaa atgtgaattt cagcgacgtt
     6781 tgactgccgt ttgagcagtc atgtgttaaa ttgaggcaca ttaacgccct atggcacgta
     6841 acgccaacct tttgcggtag cggcttctgc tagaatccgc aataatttta cagtttgatc
     6901 gcgctaaata ctgcttcacc acaaggaatg caaatgaaga aattgctccc cattcttatc
     6961 ggcctgagcc tttctgggtt cagttcgttg agccaggccg agaacctgat gcaagtttat
     7021 cagcaagcac gccttagtaa cccggaattg cgtaagtctg ccgccgatcg tgatgctgcc
     7081 tttgaaaaaa ttaatgaagc gcgcagtcca ttactgccac agctaggttt aggtgcagat
     7141 tacacctata gcaacggcta ccgcgacgcg aacggcatca actctaacgc gaccagtgcg
     7201 tccctgcagt taactcaatc catttttgat atgtcgaaat ggcgtgcgtt aacgctgcag
     7261 gaaaaagcag cagggattca ggacgtcacg tatcagaccg atcagcaaac cttgatcctc
     7321 aacaccgcga ccgcttattt caacgtgttg aatgctattg acgttctttc ctatacacag
     7381 gcgcaaaaag aagcgatcta ccgtcaatta gatcaaacca cccaacgttt taacgtgggc
     7441 ctggtagcga tcaccgacgt gcagaacgcc cgcgcgcagt acgataccgt gctggcgaac
     7501 gaagtgaccg cacgtaataa ccttgataac gcggtagagc agctgcgcca gatcaccggt
     7561 aactactatc cggaactggc ggcgctgaat gtcgaaaact ttaaaaccga caaaccacag
     7621 ccggttaacg cgctgctgaa agaagccgaa aaacgcaacc tgtcgctgtt acaggcacgc
     7681 ttgagccagg acctggcgcg cgagcaaatt cgccaggcgc aggatggtca cttaccgact
     7741 ctggatttaa cggcttctag cgggatttct gacacctctt atagcggttc gaaaacccgt
     7801 ggtgccgctg gtacccagta tgacgatagc aatatgggcc agaacaaagt tggcctgagc
     7861 ttctcgctgc cgatttatca gggcggaatg gttaactcgc aggtgaaaca ggcacagtac
     7921 aactttgttg gtgccagcga gcaactggaa agcgcgcatc gtagcgtcgt gcagaccgta
     7981 cgttcctctt tcaacaacat taatgcttct atcagtagta ttaacgccta caaacaagcc
     8041 gtagtttccg ctcaaagctc attagacgcg atggaagcgg gctactcggt cggtacgcgt
     8101 accattgttg atgtgttgga tgcgaccacc acgctgtaca acgccaagca agagctggcg
     8161 aatgcgcgtt ataactacct gattaatcag ctgaatatta agtcagccct gggtacgttg
     8221 aacgagcagg atctgctggc actgaacaat gcgctgagca aaccggtttc cactaatccg
     8281 gaaaacgttg ccccgcaaac gccggaacag aatgctattg ctgatggtta tgcgcctgat
     8341 agcccggcac ccgtcgttca gcaaacatcc gcacgcacta ccaccagtaa cggtcataac
     8401 cctttccgta actgatgacg acgacggggc ttcggccccg tctgaacgta aggcaacgta
     8461 aagatacggg ttatctgccg cattcttccc ccttctcgct tcaatttcga ccagccatcc
     8521 tctattctga tgggtattta ccacaggtcc cggaagacaa aaatgaaacg gacaaaatcc
     8581 atacgccacg catcgttccg caaaaactgg agcgcacgcc atctgacacc agtcgctctc
     8641 gcggttgcca ctgtttttat gctggctggc tgtgaaaaga gtgatgaaac agtgtctctc
     8701 tatcaaaatg ctgacgactg ttcagctgca aacccaggca aaagcgccga atgtaccacc
     8761 gcgtacaaca atgcgctgaa agaagccgaa cgtactgcgc cgaaatacgc cacccgtgaa
     8821 gactgtgttg ctgaatttgg tgaaggtcag tgccagcagg caccagccca ggctggcatg
     8881 gcaccagaaa accaggcgca ggcccagcaa tccagcggga gtttctggat gccgctgatg
     8941 gccggttaca tgatgggtcg tctgatgggc ggcggtgcgg gatttgcaca gcagccgctg
     9001 ttctcctcga aaaacccagc cagcccggct tacggtaaat ataccgacgc gacgggtaaa
     9061 aactatggcg cagcccagcc aggccgcacc atgaccgtac cgaagacagc catggcacca
     9121 aaaccggcga ccaccactac cgttacccgt ggcggttttg gtgaatctgt tgccaaacaa
     9181 agcactatgc agcgtagcgc aaccggtacc tcttctcgtt caatgggtgg ctgataccga
     9241 tggaaagagt cagtattacc gagcgcccgg actggcgtga gaaagcccac gaatacggtt
     9301 tcaattttca caccatgtac ggcgagccgt actggtgtga agatgcttac tacaagttga
     9361 ccctcgccca ggttgaaaag ctggaagaag tcaccgccga actgcaccag atgtgcctga
     9421 aagtggtgga aaaagtgatc gccagcgatg agctgatgac caaattccgc attccaaaac
     9481 acacctggag ttttgtgcgc cagtcatggc tgacgcacca gccatctctt tattcgcgtc
     9541 ttgatctggc gtgggatggc actggcgaac ctaaacttct ggaaaataac gccgatacgc
     9601 caacgtcact atacgaggcg gcgttctttc agtggatctg gctggaagat cagcttaacg
     9661 ccggtaactt gccggagggc agcgaccagt ttaacagtct gcaagaaaaa ctgatcgatc
     9721 gcttcgttga gctgcgtgaa cagtatggct tccagttgct gcatctcacc tgctgtcgcg
     9781 acacggtgga agatcgcggt accattcagt atttgcagga ctgcgcgacg gaagctgaaa
     9841 tcgctaccga gttcctctac attgacgata tcgggttagg tgaaaaaggt cagttcacgg
     9901 atttacagga tcaggtaatt tccaacctgt tcaagctgta tccgtgggaa tttatgttgc
     9961 gtgagatgtt ttccaccaag ctggaggatg caggcgtacg ctggctggaa ccggcgtgga
    10021 agagcattat ctccaacaag gcacttctac cgctactgtg ggagatgttc ccgaatcacc
    10081 cgaacctgct gcccgcttat tttgcggaag atgatcatcc gcaaatggaa aaatatgtgg
    10141 ttaaaccgat cttctcccgt gaaggcgcaa acgtgtcgat cattgagaac ggcaaaacca
    10201 ttgaagcagc ggaaggtccg tatggcgaag aagggatgat tgttcagcaa ttccacccgt
    10261 taccgaaatt cggcgacagc tatatgctga ttggtagctg gctggtgaac gatcaacccg
    10321 ccggaattgg cattcgtgaa gaccgtgcat tgatcaccca ggatatgtct cggttttatc
    10381 cacatatttt tgttgaataa tcaatgatgc cggatggcat tcgccatccg gtaattgtta
    10441 acctatctgc accgacagca tacttaaact gcccatttct ataccatcga ccggaatggt
    10501 aattggctcc tgcccatccc acgcacctaa gacatacagc aacggcagat agtgctctgg
    10561 cgttgggttc gataacgcgc caccttcatg gtcgagataa ttcaccagag gatgctgctc
    10621 taccggccct tgccacgtca gattcgcttt cacatactca ttaaacgaca tcgcccaagg
    10681 atacggtgaa ctatcaccgt gccacttcac tgtacgcagg ttatgcacca cgttgccgct
    10741 ggcgaccaac attattcctt catcacgcag cgccgcgagt tttcgcccca tttcgaaatg
    10801 ccaggcggca ggtttgctac tgtcgatact caactgcacc atcgggatat ccgcgtcagg
    10861 atacatctta atcagcacgc cccaggaacc gtggtcaaag ccccaggctt ctttatccag
    10921 cgtcaccggg atcggcgcta acagctcaac cagacgctgt gccagcgcag gcgaacccgg
    10981 agcaggataa tgcgtatcgt acagcgcctg cgggaagcca ccaaagtcat gaatcgtggg
    11041 cggcgtctcc atcgcggtca ctcctgttcc acgggtaaac cagtgagccg aaactaccac
    11101 aatcgcttgc gggcgtggca atgtcatccc caacgtctgc cagctgcggg tatacaaatt
    11161 atcttccagc acgttcatcg gactaccgtg acctaaaaac aatgctggca tacgtgttga
    11221 agacatgatg atatccttaa ctaaaggtgt cattttgata tcctcacgat acgcttgttc
    11281 ggcggagtaa gaacccggat aacaatgatg atgatcatca gttattttga cgatctgcct
    11341 gaaggtgaag atttataagg agttgtcgat gtcagtacct ctcattctga ccatactggc
    11401 gggggcagcc acgtttattg gcgcgtttct cggcgttctc gggcaaaaac cctcgaaccg
    11461 cttactggcg ttttcgctag gatttgcggc ggggatcatg ttgctcatct cattaatgga
    11521 aatgcttcct gccgcactag cagctgaagg aatgtcgcct gtgttgggtt atggaatgtt
    11581 tatcttcggt ctgcttggct attttggcct ggaccgcatg ttgccgcatg cgcatccgca
    11641 ggatttaatg caaaaatcgg tgcagccgtt gccaaaatcg atcaagcgca cagccattct
    11701 gctcactctc ggcatcagtc tgcataactt cccggaaggg attgccacct ttgtcacggc
    11761 gagcagcaac ctggagctgg gatttggcat cgcactggcc gtcgcgttgc acaatattcc
    11821 tgaaggtctg gcggttgcag gcccggttta tgcagcaacg ggttctaaac gcacggcaat
    11881 tctgtgggcg gggatttctg gactggcaga aattcttggt ggtgtgctgg cgtggttaat
    11941 cctcggtagc atgatttccc cggtggtcat ggcagcaatc atggcggcgg tcgcaggaat
    12001 tatggtggcg ctctcggttg atgaattaat gccgctcgcc aaagagattg accctaataa
    12061 taaccccagc tatggcgtac tatgtggaat gtcagtgatg ggattcagtt tggtgctgct
    12121 acaaacggcg ggaattggtt aaaagcaaca tcagaagttg tccagcgcag ggacaacttc
    12181 tgctagtttc actaaagcct tattctacct tttcggctgt ctcttctctc gtactgttta
    12241 agtatttgtt ccgcgcttca agcagtgcgt ctctgtcctg gcgaaatgcc cgacaataac
    12301 gtttcaaatg caggaaataa cccaccacaa ccagaacaat caatacaatc cagtaccagg
    12361 cgataaacat ccttttaccc ttaatattta acaatactaa acatttagcg cataaatttc
    12421 acatatcctt tttcggatat attcatatgg tcgcataata acggaacaat tatatgattt
    12481 tgatcataat gaaaaagaat gcctgatatt aatgaaattt tctcatagaa agagaagcgg
    12541 aattaacatc ttgcatagta gggaaaaata aaaaccgaag cctgctacaa cttcggtttc
    12601 ttgattaagg cagtaaatta agcagcggtt ttcagctggc tttacgctca tgtgcctgac
    12661 ggtatgccac caggtcttca atagtcacga gcgccatgtt gtgtttattg gcaaactcaa
    12721 tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt cagctcacac agcacaccag
    12781 ccggtttgaa gcctgccagc gtcattagat caatagttgc ttcagtatga ccgccacgcg
    12841 tcagtacacc accagcctga gcgcggagtg ggaaaacgtg gcccgggcga ttcagatcag
    12901 aaggttttgc gccatcggca atcgctgcgc gaacggtcgt aatacggtca gcggcagaaa
    12961 caccggtagt cacaccttca gccgcttcaa tggtcacggt aaaaccggtg ccataggcgc
    13021 tggtgttatt ttctaccatc attggcagat cgagttgttt acggcgatct tcagtaatgc
    13081 acaggcaaac aataccacta ccgtggcgaa tggtcagcgc catctgctca acagtcatgg
    13141 tttctgccgg gaagatcata tcaccttcgt tttcacggtc ttcatcatca agcaccatta
    13201 caccgcgtcc ttcacgcagc gcagccagtg cattttcaac acgttcgaaa ggcgtaccaa
    13261 aagaggaaag tagcgtctga ttcatggtaa aaaaacctca ctaaaattat ggttaccaga
    13321 atcagggcag tcttaggagt ggcggcatat agccaaaata acgtgagcgg gtccatgccc
    13381 gacagaatcg ttactctctc ccatccggac tctaaccgtc ggccccggaa ttacaccgga
    13441 tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc tttcaactga gttgatttac
    13501 cgccggtggg gaatttcgcc ccgccctgag aataagcgga ttcactataa cgctaatgat
    13561 tagcggcagc aacgcatagc ttcacataat tctggtttat gacttaccct tatcgcacta
    13621 caatggcact caacacctat cagtacaggg aaactacaat gattgacccg aaaaaaattg
    13681 agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg aatcagggag ttcggggaag
    13741 atgtggagaa aaaaatccgc caaaccctac aagcgcagct gacgcgtctc gatctggtaa
    13801 gccgcgaaga attcgacgtt cagacgcaag tcctgttgcg gactcgtgaa aaactggcgt
    13861 tactggagca gcgcagcagt gaactggaag cgcgcaataa ttcggtagca gatcttcaat
    13921 ctcctccggc tatcccgcct attgataaag cagaataaag caacacaaaa acgggccaga
    13981 cggcccgtaa tatcagtgtt ctaattcaag ttctctggtt tgcatcatct gttggcaata
    14041 ccaggcataa cgtgcgcaaa accaggttct atgttcttca tccatgttac cagtaacggc
    14101 cagaatatcg accgggcgac cttctgcgtg cattctggca aaactacgcg ctaggaaatc
    14161 gaaattattt aaagaattaa gactatgatc cataagcaca tcctccggtt atcgcatacc
    14221 ttttaaggcc atatgctttt tctgatttca gtattcccca atcagtgtag tgcctttaaa
    14281 cataacgtgc gtaaatatat tgtccccgat cacacttttt agtgaaaaat aaaaaaccat
    14341 attaaaagac tttctcttcc agaacctctc taacatatgt tattattaac aaagacaaat
    14401 gtaagcaaga aaactatcac aaagtaaata ttgcgtcact aaatggacat tggagtgaca
    14461 tatgatttta ttcgccgact ataacacccc ttatcttttc gctatttcct ttgtactgtt
    14521 gatcggcctt ttagaaattt ttgcgcttat ctgcggtcac atgctctccg gtgctcttga
    14581 tgcacatctt gaccattatg attccataac cacaggtcat attagccagg cacttcatta
    14641 tctcaatatc ggaagattgc cagctctcgt cgttctttgc ttactggctg gtttttttgg
    14701 tcttattggc atcctgttgc agcatacctg catcatggtc tggcaatcac cactctcgaa
    14761 cctgtttgtg gttcctgtca gtttactatt tacgattatt gcggtgcatt ataccggtaa
    14821 aatagttgcg ccctggattc cacgcgatca tagctcagct attacagaag aagaatatat
    14881 tggtagcatg gcgttaatta ccggtcatca agccacttca ggtaatccct gcgaaggaaa
    14941 actcaccgat cattttggcc aaatacatta tttactactg gagcctgaag aaggaaaaat
    15001 tttcactaaa ggagacaaag tgttaattat ttgccgactt tctgcaacgc gatatttggc
    15061 ggaaaacaat ccctagcccc aaatattgta atttattaat caaaaggaaa tttaataatg
    15121 gatgatattg ttaattccgt gccctcctgg atgtttaccg cgattattgc cgtatgcatt
    15181 ctgtttatta ttggaattat tttcgccagg ctctatcgtc gcgcttcggc agagcaagca
    15241 tttgtccgta ccggcttagg ggggcaaaaa gtggtaatga gcggtggcgc aatcgtgatg
    15301 ccgatctttc atgaaataat ccccatcaat atgaatactc tgaagctgga agtcagccgc
    15361 tcaaccattg atagcctgat tacgaaagat cgtatgcgcg tcgatgtagt agtcgctttc
    15421 tttgtgcggg taaaaccttc agtagaaggg attgccaccg ctgcccagac gctggggcaa
    15481 cgcaccctgt cgcctgaaga cttacgtatg ttggttgaag ataaatttgt cgatgccctc
    15541 cgtgcaacag ctgcgcaaat gaccatgcat gagttacagg atacccgcga gaacttcgtg
    15601 cagggagtac aaaataccgt agctgaagat ctgtcgaaaa acggcctgga actggaaagc
    15661 gtttcactta ccaactttaa ccagacctcg aaagaacatt tcaatcctaa caatgccttt
    15721 gacgccgaag gtttaaccaa actgactcag gagacggagc gccgtcgccg cgagcgtaac
    15781 gaagttgaac aggatgtaga agttgcggtg cgtgagaaaa accgtgatgc actttcgcgc
    15841 aagttggaga ttgaacaaca agaagcgttt atgacgcttg agcaggagca gcaggttaaa
    15901 acccgtaccg ctgagcagaa tgcgaaaatt gcggcttttg aagctgaacg tcgtcgtgaa
    15961 gcagagcaga cgcgaattct ggctgaacga cagattcagg aaacagaaat cgaccgcgaa
    16021 caggccgtcc gctcaagaaa agttgaagct gaacgtgaag ttcgcattaa agagatcgaa
    16081 cagcagcagg tcaccgaaat cgctaaccag acgaaatcga tcgctattgc cgccaaatcg
    16141 gaacaacagt cccaggcaga agcgcgtgct aaccttgcac tcgcagaagc agtaagcgcc
    16201 caacaaaacg tagaaaccac tcgccagaca gccgaagccg atcgtgccaa gcaggtggcg
    16261 ctgattgccg cagcacagga tgcagaaacc aaagcggttg aactgaccgt gcgggcgaaa
    16321 gcagaaaaag aagccgcaga gatgcaggcg gcggcgatcg ttgagttagc cgaagctacg
    16381 cgtaaaaaag gcctggcgga agcagaagca caacgtgcgc tgaacgatgc tatcaacgta
    16441 ctttctgacg aacaaaccag ccttaaattc aaactggcct tgttgcaggc gctacctgcg
    16501 gtaatagaga aatccgtgga accaatgaag tccattgacg gtatcaagat tattcaggtc
    16561 gatggtctga atcgtggtgg tactgcgggg gatgcaaaca cgggtaatgt ggggggcgga
    16621 aacctggcgg aacaagcatt atcagccgca ctctcttacc gcacacaggc accactgatt
    16681 gactccttgc tcaatgaaat tggcgtttca ggcggctcac tggctgcatt gacttcactc
    16741 ttaaccccaa caactcccgt cgccgaaaac gtagaataaa ttttcagcta tgaacatccc
    16801 tggctcaaaa agccggggat tttttatatc tgcgttccgc taaaaggtgc aaatgatcag
    16861 gctgttgcag cgttttgcgt gattgctcgg ggaaggcaaa actgcttcag ggaaagcgtt
    16921 gcgcggggtc cggcgctcat caacaatcgg ggagtaagca aagggctgaa acgggaaagc
    16981 ccctcccgag gaaggggcct tacagaagga aagggttatg atgaagctcg tcatcatact
    17041 gattgtgttg ttactcgtaa gtttcgcagc ttattaacag ccaatcagag gggggagaaa
    17101 tcctccctca cccttgttcc tttactttag gttgaaaaaa aatcagcgtc aagaaggttt
    17161 cattaacaca tatccctggc ccaaaaagcc ggggattttt tatatctgcg ttccgctaaa
    17221 aggtgcaaat gctcaggacg ttgcagcgtt ttgcgtgacc gctcggggaa ggcaaaactg
    17281 cttcagggaa agcgttgcgc ggggtccggc gctcatcaac aatcggggag tcagcaaggg
    17341 gctgaaacgg gaaagcccct cccgaggaag gggccttata gaaggaaagg gttatgatga
    17401 agctcgtcat catactggtt gtgttgttac tgttaagttt cccgacttac taacaactca
    17461 tcagaggggg gagaaatcct cccttaccct tgttccttta ctctaggttg aaaagacaac
    17521 agcgtcaata ggcctgccat gtacgaagcg agatctgtga accgctttcc ggttagcctt
    17581 ttttatcctg ttggatcttc ttgataatgt tagtggtcga gcaaccgtct tcaaagttga
    17641 gcaccaacac ttcgccgccg ttggcccaga cttctttact cccggcgatc tcttccggtt
    17701 tgtagtcacc gcctttcacc agcaggtctg gcaggatccc ggcgatcaga cgctgcggcg
    17761 tgtcttcttc aaacgacact acccagtcga ccgcttccag tgcgcccagc acaatcatac
    17821 gctgttcgag tgggtttacc gggcgggaat cccctttcag ccgtttggtg gaggcatcgc
    17881 tgttgacggc aacaatcaag cggtcaccca gcttgcgggc atttgccaga taagagacgt
    17941 gcccggcatg taggatatcg aagacaccgt tggtcattac cactttttca ccacgtttac
    18001 gcgctgccac tacagccagc ttcagttcct cttcggtcat cacgccaaag ccggtatctg
    18061 cgcgtccacg tacagcgttt tccagctcga tcggcgaaac cgtggacgtc cccagtttgc
    18121 cgaccaccac accagccgcc gcattggcaa agaagcaggc ttcttccagc gaattacccg
    18181 ctgccagcgt tgccgccagg acgccaatca ccgtgtcgcc cgcaccggta acgtcataca
    18241 cttcctgcgc ctgagttggc atatgcagcg gcgctttacc cggttgcagc agcgacatac
    18301 cctgttcgga acgggtcact aacagagccg agagttcgta atcggcaatc agtttcatgc
    18361 cgcgctcaac aatctcttct tcggtcttac atttaccgac aacagcttca aattccgaga
    18421 gattcggcgt taacagcgta gcgccgcggt agcgctcaaa atcggtacct tttggatcaa
    18481 tcagcaccgg aacacccgct ttacgcgcca gttggatcat ctgctgtacg cttgccagcg
    18541 cacctttggc gtagtcagaa agcaccagcg cgccaatcga actcagcgcc tgattaatcc
    18601 gctcgtgcag cggctgcgga tcaacacctt cgaaaccttc ttcaaaatcc agacggatca
    18661 gctgttggtt gcgggaaagt acccgtaatt tggtaatggt cggatgcgtc ggtacagaaa
    18721 cgaagtcgca tttgacgttg acgtcggcca gagatttact cagcgcgcgc gctgcatcgt
    18781 caatgcccgt caacccgacc aggcgtgcat tagcaccgag agaagcgata ttcatcgcca
    18841 cgttagccgc gccgcccgga cgttcttcga tggtattcac tttaaccacg ggcaccggcg
    18901 cttccggcga gatacgactg gaggggccgt accagtaacg atccagcatc acatcaccaa
    18961 ccaccatcac tcctgcacgt tcaaactctg gcagcgttac tttcattcct gtctcctgag
    19021 agattcaaaa tttgcgcgcg ataataccat acttcattct tccaccagcc acttctgcca
    19081 gcttgcccgc accagttcac gctctgcggt gaagcaatcc tccggcacat ggcccggcaa
    19141 ttcctgtaat gccagatgat gaagttcatc gcgaagcgta gtgtaagcac gggtcagcgc
    19201 catcgcttcc tgctcttcca taatgtcgtt ttgcgccagt agttccagaa tgcgcacgtt
    19261 gtctgaccag cgcgttaact tcggtttttc atgagcgtag cgcaacacca gatattgggt
    19321 aataaattcg atatcggtaa ttcccccttc atcagctttg atatcaaagc gatcgcgatg
    19381 tttattgccg agatgagcgc gcattttctc gcgcatttcc cgcacttccg tttgcagagt
    19441 tttaccttca cgcggcagcg tcataatctc gcgacgcact gcgtcaaagt gcgcggtgag
    19501 ctgcggatcg ccgtacacta cacgcgcacg caccagcgcc tgatgctccc acgtccaggc
    19561 ctcgtttttc tgataatcgg caaatgcttc tgtggaggta accagcattc ccgccgcccc
    19621 ggacgggcgc agccgcgcat ccacttcgta caaaatgccg gaagaggttc gcgtgctgaa
    19681 caggtgcata atgcgttgcg ccagacgcag ataaaactgt cgtccgtcga tttcccgctc
    19741 gccgtcagtc atcgcatcca ttgggcaatc atggaggaag atcaggtcaa gatcggaact
    19801 gtagcctaac tcccagccgc ccagcttgcc gtagccgacc actgcaaaac cacgcccttc
    19861 gcgttcgttc aggtgattcg gcttaccgta gcgggcaacc atttgaaccc acgcctgctg
    19921 aacgacggca tctatcatgg cttccgccag ccaggttaag tgatcgctca ctttcatcac
    19981 cggtagcgta ccggcgatat ccgctgcggc gatgcgtaac agctgcgcct gtttgaactg
    20041 acgcagcgcc tcaagctgtt gctcttcgtc atcttccggc acgcgcagca aatactggcg
    20101 caactcatcg cggtaggcat cggtcgccgt cggctggtaa agggtgtttg gatcgagcaa
    20161 ttcatccagc aataatggat aacgcgccag ctggctggca atcatcggcg acgctgcaca
    20221 cagagaaatc aaatgtttaa gcgccgcggg gaattcactg agcaattcta aataggtggt
    20281 gcgggtaaca atccccgcca gcaaggcggt aatgcgcaac agcgtaacgg cagcgtcttc
    20341 acgcgcacag acatcactta gcagatgcgg catcagatgg tcgagcacct gacgcccgcg
    20401 tggcccgatt gtgcgcttat ccagctcttt gcggaaatcg gcaatcagcg tcagcacctg
    20461 tttgcgatcg tcctcgctaa gatgcgccag cactggtgta gtgtcatctt cctgcaacgc
    20521 atcctgccac agctcgcgcc actgttccga cagcgactct tcctgagttt cactttcgtc
    20581 gtcgccaatc aattcattaa acacccggcg cacattggtc atatgtgcgg tcagcgcccc
    20641 ggtcaattgc ggccagtcag caaagtccat cgcccacgcc agccgcgcac ggttaagctc
    20701 atcagaagga agcgtctggg tttgttcgtc gttaatgctt tgcagcaggt tttccagacg
    20761 ccgcaggaac agatacgcca ctcgcaattg ttcagcatcg ttttcagaaa gcagatgcag
    20821 cgcggcaatg acgctgagcg ttggcagtaa agagcgcgac tgcagcgacg gttcgcgccc
    20881 gccgcgaatg agctggaaca cctgaacgat aaattcaatt tcgcgaatgc cgcctgcgcc
    20941 gagtttgata ttgtcggtca aaccacgtcg acgcacttca cgggcaatca ttcctttcat
    21001 gttgcgcagc gactgaatca cgctgaaatc gatgtaacga cggaaaacaa acgggcgcag
    21061 catcgcacgc aactcgttag catagacgcc ttcgctatcg cccataatcc gcgccttgac
    21121 catcgcgtaa cgctcccagt cgcgcccctg ctcctggtaa taatcttcca acgcggcaaa
    21181 gctcagcacc agcgggccac tttcgccaaa cggacgcagc cgcatatcca cgcgatagac
    21241 gaagccatcc tgcgttggtt gatccagcac tttaatcagc cgctgcccca tgcgggtaaa
    21301 aaactgcgcg ttatccagtt cccagcgtcc accctgcgta caaccatgtt ccggccaggc
    21361 aaaaatcaga tcgatatcag aggagaaatt cagttccccg ccgcccagct tacccatgcc
    21421 taaaatcagc agcggttgcg cttcgccctg cgcattgcac ggcgttcccc actcgcggca
    21481 gcaggcgtca tatagccagt cacgcgccgc aacaatcagc gtctccgcca gatagctgag
    21541 ctgctgcaat atgctctctt cagtaaccag tgccagcgtt tgcgcccagg cgatacgtac
    21601 catgatgcgc cgacggaaca gccgcagctc gcgcattaac ccggcttcgt cactcacatt
    21661 aatgagcgcc tcctgcaacc atgcaacgta atgctgccat tcgtcggcct gcggcggttg
    21721 gctttccagt tccgtcagcc actctggatg cgcacttatg ctgtcctgca caaaatcact
    21781 gaaagtaagt actgacttcg cctgtgcact aagtgattcc tcggctaaag gctctggcag
    21841 ccgctcaaca atggtctgcc agtactgctg taacggtgaa gagagcggct tcataagcga
    21901 ttttatcctt gcctgattaa cgttttccgc tgtgcaacca gaacggttcc tgattgtttg
    21961 cctcattacg gaaatgttca atttcgatgc gttgcccggt cgcaatagcg tgatgcagcc
    22021 cctgccagtt ctccagccag gcttgcgcga cgacaggatc atagtaaccc gccagcaaca
    22081 gtattgagtc aatatcacgc gtcaggcgtg gcaactggtc acggtagcga tcgcctaacg
    22141 gctggcagaa aacgcttttc agttcagcgg catggcggga aagatggata tcggcaaagc
    22201 gtttgaagga gtcgctgatt ttgccctgcg ctttggcatc taaaaatggc tgccatgctt
    22261 tgcttaccaa ccattcggtc aacgccagct tcgccattgc cgtttcggta gagtagaccg
    22321 ccgtcacggc agaaaccgca gaagcaatgg tcgcttcgca ttgggtcagc aaatcacgta
    22381 agtgagtgct cgctttacgc ggcacaatac caccgaacag catcagcgta tgacggacca
    22441 agctaatggc tgccagcacc tgttctttcg ccgcatcgtt gccgcgtacc cacagttctt
    22501 catgatactg ccattgcgct aacgccagct cgagcgccgc ttccagcccc tgttccacat
    22561 cggcttttgc cgcaacatgc aaaatggtgg tcggtttgat ttcacgcgcc ggattgccct
    22621 gcgccagatg atagccacgc gccgctttgc tcaggctgcc ctggcgtaat ccggtttgcg
    22681 ataccagttg gttcgccagt ttcagcaccg cgcgcgtgtc gccgctaagc agctccagtt
    22741 ccagctcaca gataggttca gcaaattccc ccgctttcac ctcaccctga tcgagagcga
    22801 tttcaattcg gctgccatcg accgccacca gccatttttc gcgataaaaa tcggtgctga
    22861 acagcggttg tacgcgggaa gcgagatcgg cgggcaattc gccgttcggc cagacttccg
    22921 tcggtaactg cgccaggtcg agcgtcggtt cgctcaacgc cacgttatat tccgggcgct
    22981 ggtgtaagcc gcctgtcact cttcctgcaa ctttcatggt catctcatag cgaccgtttt
    23041 cgccacgaat acgtaagccc atatcgtgcc cacgcagcca gttatccggc gtttcgtagt
    23101 aaatattcag taactgcacg gggtcatggt gctcgccgcc cagcgtattg agatggtcac
    23161 gcaacgcctc aacggcgctg tgattaacaa taaactttaa ttcgatttcc tgagccatgg
    23221 cctttactta tcggttatgt cacataagtg acgatgaacg gcgaacttaa tgcgatcttt
    23281 tttgtcagta gatagtattt tgcgccaaat tgccatgcaa cgggcaattt gacgggcgta
    23341 aaagtttgaa gcagtggcaa gacctgacac agaggattcc gattgatgtc gaatgctttg
    23401 cgtagcgaca ctgatgccac tactatcgtt ccactttcca tgacaataac gacagcctga
    23461 tgccaaaatt acgcctgatc ggattaactt tactcgcact tagcgcgact gccgtctcac
    23521 acgctgaaga aacgcgctat gtttctgacg aactgaatac ctgggtccgt agcggtccgg
    23581 gagatcatta tcgcctcgtg ggcacggtta acgccggcga ggaagtgacc ttattacaaa
    23641 ctgacgccaa caccaattat gcccaggtga aagacagctc tggccgtacc gcctggatcc
    23701 cgttgaaaca acttagcact gagccaagcc tgcgctcccg tgtgccagat ctggaaaatc
    23761 aggtcaaaac cctgaccgat aaactcacca atatcgataa cacctggaat cagcgcaccg
    23821 cagaaatgca gcaaaaagtg gcgcagagcg acagcgtgat caacgggtta aaagaagaaa
    23881 atcagaaact gaaaaacgag ctgattgtcg cgcagaaaaa ggtcgatgcc gccagcgtgc
    23941 agctggatga caaacagcgc accatcatca tgcaatggtt tatgtatggc ggcggcgtgc
    24001 tggggcttgg cttgctgctc ggtctggtac tgccgcacct gatcccaagc cgcaaacgca
    24061 aagatcgctg gatgaactaa atcgccttct tcgttgcact gtcttattat taggaattgt
    24121 gtatttgacg aagggatgat ggcgtgaaga tttatctggt cggtggtgct gttcgggatg
    24181 cattgttagg gctaccggtc aaagacagag attgggtagt ggtcggcagt acgccacagg
    24241 agatgctcga cgcgggctac cagcaggtag gccgcgattt tcctgtgttt ctgcatccgc
    24301 aaacgcatga agagtatgcg ctggcacgta ccgaacggaa atccggctcc ggttacaccg
    24361 gttttacctg ctatgccgca ccggatgtca cgctggaaga tgatcttaag cgtcgcgatc
    24421 tgaccattaa tgcgctggcc caggacgata acggcgagat tatcgacccg tacaacggtc
    24481 tgggcgatct gcaaaatcgt ctgttgcgcc atgtttcccc cgcttttggc gaagatccgt
    24541 tacgcgtact gcgcgtggcg cgttttgctg cgcgttatgc ccacctcggt tttcgtattg
    24601 ccgatgaaac tctgacgctg atgcgcgaga tgacccatgc gggtgaactg gaacacctga
    24661 cgcctgaacg ggtatggaaa gagacagaaa acgccctcac cacccgcaat ccacaggtgt
    24721 tcttccaggt actgcgcgat tgcggcgcac tgcgcgtttt attcccggaa attgacgccc
    24781 tgtttggtgt tccggcccct gccaggtggc atccggaaat cgacacgggt attcatacct
    24841 taatgacgct ctcaatggcg gcgatgctga gtccgcaggt cgatgtccgt ttcacaactt
    24901 tatgccacga tctcggtaaa ggactgacgc cgccagaact ctggccgcgt catcatggtc
    24961 atggcccggc gggtgttaag ttagtggaac aactatgcca gcgtctgcgc gtgccaaatg
    25021 aaattcgcga tttagccaga ctggtggctg agtttcacga tctcatccac accttcccaa
    25081 tgctgaaccc aaaaaccatc gtcaaattat ttgattccat cgatgcctgg cgtaaaccgc
    25141 agcgtgtcga gcaactggcg ctgaccagcg aggctgacgt gcgcggcaga accggttttg
    25201 aatcagcgga ctacccgcaa ggccgctggt tgcgcgaagc ctgggaagtg gcgcagtcag
    25261 tgccgacaaa agccgtcgtt gaagcgggat ttaaaggtgt ggagattcgc gaggagctga
    25321 cccgacggcg gattgcggcg gtagccagct ggaaggaaca acgttgccca aagcctgagt
    25381 gagtattggg cattttgccg gatgcggcgt aaacgcctta tccggctaca tgtcaacgac
    25441 agttgtaggc ctgataagac gcgcaagcgt cgcatcaagc gttgattgcc ggacgcggcg
    25501 tgaacgcctt attcggccta cgcaatcgtg gacaacgcca ggcatccgac actattcctc
    25561 aattaaaaga acacgacata caccgcagcc gccacaataa agcgataaat ggcgaacggg
    25621 ataaacgaaa tgcgcttaat caattgcagg aaggttttaa tcgctatcag cgccaccaca
    25681 aaagcggtga taaacccaac ggcaaacatc gggatatcgc cgcttgtcag gaagccccag
    25741 cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg gcaccgccag caggaacgaa
    25801 aactcggaag cagcgtaacg gctcaccccc atcagcatcc cacctgaaat ggtcgccccg
    25861 gaacgggaga aacccggcca cagcgccaga cactggaaac aaccaatcat aaacgcctga
    25921 cgataggtca tatcatcaag acccggcgca cgcggctctt tcggcttcag gcattcggcg
    25981 gcaatcagca acaaaccgcc aacgaccagc gcatacatca catttatcgg gttaaacaat
    26041 gacttaatcg tgtcgtggaa caacagcccc agtaccaccg ccggaatcat ccccagcaaa
    26101 atgtggatca gcgttaaacg acctttgctt tctccttcgt gctgcaacgg gcggccaaag
    26161 tggatgccaa tcaggccaaa cagacgccgc cagaacatca ctactaccgc cagaattgat
    26221 cctaactgga tcacaacttc aaaggttttc gccgtgtcgc cctcaaaccc caacaagtga
    26281 ccgacaataa tcatatggcc cgtgctggat accggcagaa attctgtcaa tccttcgacc
    26341 acacccaata ttgccgctat cagcagcgag tgcatatcgc tcatcaataa acccctaaat
    26401 tataaaaatg taacgcttgt ccgaactact gcagtatgac caggttataa ccgtttggtt
    26461 taacagctgt aaaattaatt attttctttc agattattgc cacgctcaat gattacgcca
    26521 acattcgccg cccgcgccac tgcgcctggc ttgctgagtt tgatacgcac ccacggcgag
    26581 ttgaagcgtg ctaacagcag ctctgccacc tcttccgcca cgcgctccac cagcgcaaaa
    26641 cgtgccccct cgacgtggct gaccaccgtt tctgcaatgt cagcgtagct gaggcaatcc
    26701 gccacattat cgctttttgc cgctttacgg ttatcccacg ccatttcgat atcgaacact
    26761 aatttctgtt cgatggtctg ttcccagtcg taaacaccaa tagtggtgat taccgaaagt
    26821 tgctctataa atacaatatc catcacgacc tgctcgcttt ttggctaacc cggataccac
    26881 ttccggcgaa atgtgcgtat tatccacaga ttcatcgttt aacacgaatt ttcaaaacgg
    26941 aacagcttat gagtgcaatc gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca
    27001 tttccagtgc cattctggtt tgccgcctgt gtgggctgcc cgatccgcga accagcggct
    27061 ccggtaatcc tggcgcaact aacgtgttac gtataggtgg caaaggagca gccgtagcag
    27121 tactgatttt cgacgttctg aaaggaatgt tgcccgtctg gggcgcgtat gaattaggtg
    27181 tcagcccctt ctggctaggc ttaattgcca tcgccgcctg tcttggacac atctggcccg
    27241 ttttcttcgg atttaaagga ggaaaaggcg ttgctaccgc ttttggtgcc atcgcaccca
    27301 ttggctggga tctcaccgga gtaatggcgg gaacctggtt actgaccgtg ctattgagcg
    27361 gatactcgtc gctgggagcg attgtcagtg cactgattgc tccgttttat gtctggtggt
    27421 ttaagccaca attcaccttc ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc
    27481 atgacaacat ccagcgtctg tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa
    27541 gaaagcgcga aaaggatccc gagtgatttc tggtggatct acatgacctg atagccttca
    27601 tcgggtttgc ccagccgttg ctggcaccat gccgccagaa attccacgca gacacgtaat
    27661 ttcatgctgc gatagagcgg ctcccggtaa acagcccaga tattggcgct ttgtgcatac
    27721 tccggcaata cttgcaccag tttgccactc tccagaaacg gcaacacatc ccactcggaa
    27781 cgcagcataa tccctttgcc ctccagcgcc cattgcagca caatctcgcc gctattggag
    27841 gaaagatgcc cgctaacctt caccgatttt ttctcctgac cgttccccaa ctcccatatt
    27901 ccgtgggtca tatcgcgctc tttggtcacc agacagtcat gacgacttaa ttcctgtagg
    27961 gattgtggct gcggatattt ttgcagatac tcaggtgctg cgcataatat tcttttattt
    28021 ttcgttaaca gatgcgcaat ataataatca ggaatttcgt cattaatacg aatatccaga
    28081 tcaatattat cctgcactaa atcaatttgc cgatcgaaca attcaaaata cacctgtaac
    28141 tcaggataat tgcgcataag ttcggtaatg gctggcgcaa tatggctgcg cccaaaacca
    28201 aaactgcaac caatacgaat catcccctcc gggcgcgttt tgatttgcgt gacgtcatcg
    28261 accagtcgct gatattgggt gaggatttcc agcgcatgtt cgtaacaacg ttggccactt
    28321 tcagtcagcg ccacgccgcg agcggagcgg ttcagcagcg ttgttgccag cgtattttca
    28381 aggatttgga tccgcttggt gacaaatgca ggcgtttgcc caagcgtcgc cgctgcagca
    28441 ctaaaactcc cgctgtggac gatttctacc agtacctgca agtctttggc taaaggccag
    28501 ctattcagca tcgttaatta tccgcagttg tgataagcgc agtgtatttc gcaaaacatt
    28561 gccattccct gctctggcgc aatattcatt cttattttcg cgttgattca cgaactgtta
    28621 attacatatt cacggtggca aaaaatataa aaccacattt ttagtggtag tttgtggcgg
    28681 tgaatttttc cagacaaata caaaaactgg agttgccatg atgagcgaaa gtaataagca
    28741 acaggcagtg aataagttga cggagattgt cgctaacttt accgccatga tttctacccg
    28801 aatgcctgat gacgtggtgg ataaactaaa acagctaaag gatgccgaaa cgtcgtcgat
    28861 ggggaaaatt atctaccata cgatgttcga caacatgcaa aaagcgattg acctgaatcg
    28921 tcctgcctgt caggacaccg gggagattat gttcttcgtt aaagtcggtt cccgcttccc
    28981 actgcttggc gagctgcaaa gcatactcaa acaagccgtg gaagaggcga ccatcaaagc
    29041 gccgctgcgt cacaatgcgg tagaaatttt tgacgaagta aacaccggca aaaataccgg
    29101 tagcggcgta ccgtgggtca cctgggatat cgtccccgac ggtgacgatg cggaaatcga
    29161 agtttacatg gcaggcggcg gctgcacgct accaggccgc tcgaaagtgt taatgccgtc
    29221 agaaggctac gaaggcgtgg tgaaattcgt cttcgaaaat atctccaccc tcgcagtaaa
    29281 cgcctgtcca ccggtactgg tgggcgtggg catcgccacc tcggtggaaa ccgccgccgt
    29341 actctcgcgt aaagccattt tgcgcccgat tggcagccgc caccccaatc caaaagcggc
    29401 agagctggag ctacgcctgg aagaaggact caaccgtctg gggattggtc cacaagggct
    29461 gactggcaac agttcagtga tgggcgtgca tatcgaatct gccgcccgcc atccgtcaac
    29521 catcggcgtt gctgtttcta ccggttgctg ggcgcatcgt cgcggcacac tgctggttca
    29581 tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa aaagatcctg
    29641 acaaccccga tcaaagctga agatctgcaa gatattcacg tcggcgatgt gatctatctg
    29701 accggtacgc tggtgacctg ccgtgacgtt tgtcaccgcc gattgatcga actgaaacgt
    29761 ccgctccctt acgatctcaa cggcaaagcg attttccacg ctggccccat cgtgcgcaaa
    29821 aacggcgaca aatgggagat ggtctccgtc ggtccgacaa ccagtatgcg tatggaaagt
    29881 tttgaacgtg aatttatcga gcagaccggc gtgaaactgg tggtcggcaa aggcggaatg
    29941 gggccgctga ccgaagaagg ctgtcagaaa ttcaaggcgc tacatgtaat tttcccggca
    30001 ggctgcgcgg tgctggcggc aacccaggtg gaagagattg aagaagtgca ctggacagaa
    30061 ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc gctgattgtc
    30121 tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt cgccgaacgc
    30181 cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata accctcccgg
    30241 agaggctcgc cccctctcct ttttcgcagg cataacacga tgaaaccttc cactgaatgg
    30301 tggcgatacc ttgcgccgct ggcggtcatc gccattattg ctctaattcc ggttcccgca
    30361 gggctggaga gtcatacctg gctctacttt gccgttttta ctggcgtaat cgttggactg
    30421 atcctcgaac ccgtgccggg tgccgtggtg gcgatggtgg gtatctccat catcgccata
    30481 ctctctccct ggctgctgtt cagcccggag cagctcgctc agccaggctt taaattcact
    30541 gcaaaatccc tctcgtgggc cgtttccggt ttttctaatt cggttatctg gctgattttc
    30601 gccgccttta tgtttggcac aggctatgaa aaaaccgggc tggggcgacg tatcgcgctg
    30661 attctggtga aaaagatggg gcatcgcacg ctgtttcttg gctatgcggt gatgttctcc
    30721 gagctgatcc tggcacctgt cacaccgtcc aactcggcgc gtggtgcggg gattatctat
    30781 cccatcatcc gtaacctgcc gccgctctat caatcacaac caaacgacag cagttcgcgc
    30841 agtattggct cgtacatcat gtggatgggg attgttgccg actgcgtgac cagcgccatt
    30901 ttcctgacgg cgatggctcc taacttgctg ttaattggac tgatgaaaag cgcatctcac
    30961 gccacgctga gttggggcga ctggttcctc gggatgttgc cgctcagtat tttactggtc
    31021 ctgctggttc cctggctggc ttacgtgctg tacccgcctg tactgaagtc aggcgatcag
    31081 gtgccgcgct gggcagagac ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa
    31141 cggatgctgg ggctgatggt aggcgcgctg gtgctgtgga ttttcggcgg tgattatatc
    31201 gatgccgcga tggtcggtta cagcgtggtg gcgctgatgc tgcttctgcg cattatcagt
    31261 tgggacgata ttgtcagtaa taaagcggcg tggaacgttt tcttctggct ggcctcgctt
    31321 atcaccctcg cgaccggact caacaacacc ggttttatta gctggtttgg caaactgtta
    31381 gcaggcagct taagcggtta ttcgccaacg atggtgatgg tggcgttgat tgtggtgttt
    31441 tatctactgc gctacttttt tgccagcgcc acggcgtata cctccgctct cgcaccgatg
    31501 atgattgccg ctgcgctggc gatgccggaa atcccgctgc cggtgttctg cctgatggtt
    31561 ggtgcggcaa ttggtctggg gagcattctt acaccatacg ccaccggccc cagtccgatt
    31621 tactacggca gtggttatct gccaacggcg gattactggc gactgggggc gatttttggg
    31681 ctgatattcc tcgtattgct ggtgattacc ggcttactgt ggatgcccgt ggtgttgctt
    31741 taagtaagga cggtgccgga agtaactctc cggcacccga tagttttacg cagccggtaa
    31801 ctccgccagc ggccagcgcg gacgcacgct aacgccgaga tccgccgtcg cgcctgcttt
    31861 aaaccgcacc attccggcat aggcgatcat cgcgccgtta tcagtacaaa attccggacg
    31921 cgcgtagaac acttcgccgc ggcgtttttt catcatttca gccagcttcg cccgtaacgt
    31981 gcggttagca ctcacgccgc ccgccatgac cagtcgctta aagcccgtca gatccagcgc
    32041 acgcttacac ttaatcatca acgtatcgac caccgcatct tcaaaggcgc gggcgatgtc
    32101 agcacgcgtc tggtcgtcgg tgccgttgtc acgaatggta tttgccgcga aggttttcag
    32161 gccggagaag ctgaaatcca gccccggacg gtcggtcatc ggacgcggga aaacaaagcg
    32221 cccggcagta ccctgcgccg ccattttcga cagtaacggt ccgccaggat aatccagccc
    32281 cagcagcttc gcggttttat caaacgcttc accggcggca tcatcgatag actcgccgag
    32341 cagctcgtac tgaccaatgc cagtcacgct gattaactgc gtatgaccgc cggaaaccag
    32401 cagcgcaaca aacggaaatt ccggcgggtt atcttccagc atcggcgcta acagatgccc
    32461 ttccatatgg tgtacaggga tcgccggaac gttccaggca aacgccagag aacgccccac
    32521 ggtcgcgcca accagcagtg cgccgactaa tccagggcct gcggtatagg ccacagcatc
    32581 aatgtctttt gccgttaaac cagactcctt tagcgccgcc tggatcaacg gtacggtttt
    32641 acgcacatga tcgcgggagg ccagttcagg cacaacgccc ccgtagtcag cgtgcaattt
    32701 cacctgacta tacaattggt tggctaacaa acctttttca tcgtcgtaaa tggcgatgcc
    32761 ggtttcatcg caggaagttt caatacccag tacacgcatg acttgtttta cctcgcttta
    32821 ttaccgcgca gtgtaggacc aatgcgggtt gatgtaaaac tttgttcgcc cctggagaaa
    32881 gcctcgtgta tactcctcac ccttataaaa gtccctttca aaaaaggccg cggtgcttta
    32941 caaagcagca gcaattgcag taaaattccg caccattttg aaataagctg gcgttgatgc
    33001 cagcggcaaa ccgaattaat caaaggtgag aggcacatgc cggtaattaa agtacgtgaa
    33061 aacgagccgt tcgacgtagc tctgcgtcgc ttcaagcgtt cctgcgaaaa agcaggtgtt
    33121 ctggcggaag ttcgtcgtcg tgagttctat gaaaaaccga ctaccgaacg taagcgcgct
    33181 aaagcttctg cagtgaaacg tcacgcgaag aaactggctc gcgaaaacgc acgccgcact
    33241 cgtctgtact aattccccga gagcgttgct ctccgatcag accgagttgt agttgtaagg
    33301 ccgtgcttcc gaaaggaatg cgcggcttat tttcgtttat gaattgctaa aaatcggggc
    33361 ctatggctgg acgaatccca cgcgtattca ttaatgatct gctggcacgc actgacatcg
    33421 tcgatctgat cgatgcccgt gtgaagctga aaaagcaggg caagaatttc cacgcgtgtt
    33481 gtccattcca caacgagaaa accccgtcct tcaccgttaa cggtgagaaa cagttttacc
    33541 actgctttgg atgtggcgcg cacggcaacg cgatcgactt cctgatgaac tacgacaagc
    33601 ttgagttcgt cgaaacggtc gaagagctgg cggcaatgca caatcttgaa gtgccatttg
    33661 aagcaggtag cggccccagc cagatcgagc gccatcagcg gcaaacgctt tatcagttga
    33721 tggacggtct gaatacgttt taccaacaat ctttacaaca acctgttgcc acgtctgcgc
    33781 gccagtatct ggaaaaacgc ggattaagcc acgaggttat cgctcgcttt gcgattggtt
    33841 ttgcgcctcc cggctgggac aacgtcctga agcggtttgg cggcaatcca gaaaatcgcc
    33901 agtcattgat tgatgcgggg atgttggtca ctaacgatca gggacgcagt tacgatcgtt
    33961 tccgcgagcg ggtgatgttc cccattcgcg ataaacgcgg tcgggtgatt ggttttggcg
    34021 ggcgtgtgct gggcaacgat acccccaaat acctgaactc gccggaaaca gacattttcc
    34081 ataaaggccg ccagctttac ggtctttatg aagcgcagca ggataacgct gaacccaatc
    34141 gtctgcttgt ggtcgaaggc tatatggacg tggtggcgct ggcgcaatac ggcattaatt
    34201 acgccgttgc gtcgttaggt acgtcaacca ccgccgatca catacaactg ttgttccgcg
    34261 cgaccaacaa tgtcatttgc tgttatgacg gcgaccgtgc aggccgtgat gccgcatggc
    34321 gagcgctgga aacggcgctg ccttacatga cagacggccg tcagctacgc tttatgtttt
    34381 tgcctgatgg cgaagaccct gacacgctgg tacgaaaaga aggtaaagaa gcgtttgaag
    34441 cgcggatgga gcaggcgatg ccgctttccg catttctgtt taacagcctg atgccgcaag
    34501 ttgatctgag tacccctgac gggcgcgcac gtttgagtac gctggcactg ccattgatat
    34561 cgcaagtgcc gggcgaaacg ctgcgaatat atcttcgtca ggaattaggc aacaaattag
    34621 gcatacttga tgacagccag cttgaacgat taatgccaaa agcggcagag agcggcgttt
    34681 ctcgccctgt tccgcagcta aaacgcacga ccatgcgtat acttataggg ttgctggtgc
    34741 aaaatccaga attagcgacg ttggtcccgc cgcttgagaa tctggatgaa aataagctcc
    34801 ctggacttgg cttattcaga gaactggtca acacttgtct ctcccagcca ggtctgacca
    34861 ccgggcaact tttagagcac tatcgtggta caaataatgc tgccaccctt gaaaaactgt
    34921 cgatgtggga cgatatagca gataagaata ttgctgagca aaccttcacc gactcactca
    34981 accatatgtt tgattcgctg cttgaactgc gccaggaaga gttaatcgct cgtgagcgca
    35041 cgcatggttt aagcaacgaa gaacgcctgg agctctggac attaaaccag gagctggcga
    35101 aaaagtgatt taacggctta agtgccgaag agcgatcggg aagcccccga cagccgcact
    35161 gagaggcagc ggcaaatata taagtacgcc ctcgtaatta tcgttggcgg taaacaaccg
    35221 ttggatttca gcgttaacgg ctgaaggaca tcgggtcaat cgcccaacac caacctcatg
    35281 aaataagtgt ggataccgtc ttatggagca aaacccgcag tcacagctga aacttcttgt
    35341 cacccgtggt aaggagcaag gctatctgac ctatgccgag gtcaatgacc atctgccgga
    35401 agatatcgtc gattcagatc agatcgaaga catcatccaa atgatcaacg acatgggcat
    35461 tcaggtgatg gaagaagcac cggatgccga tgatctgatg ctggctgaaa acaccgcgga
    35521 cgaagatgct gccgaagccg ccgcgcaggt gctttccagc gtggaatctg aaatcgggcg
    35581 cacgactgac ccggtacgca tgtacatgcg tgaaatgggc accgttgaac tgttgacccg
    35641 cgaaggcgaa attgacatcg ctaagcgtat tgaagacggg atcaaccagg ttcaatgctc
    35701 cgttgctgaa tatccggaag cgatcaccta tctgctggaa cagtacgatc gtgttgaagc
    35761 agaagaagcg cgtctgtccg atctgatcac cggctttgtt gacccgaacg cagaagaaga
    35821 tctggcacct accgccactc acgtcggttc tgagctttcc caggaagatc tggacgatga
    35881 cgaagatgaa gacgaagaag atggcgatga cgacagcgcc gatgatgaca acagcatcga
    35941 cccggaactg gctcgcgaaa aatttgcgga actgcgcgct cagtacgttg taacgcgtga
    36001 caccatcaaa gcgaaaggtc gcagtcacgc tgccgctcag gaagagatcc tgaaactgtc
    36061 tgaagtattc aaacagttcc gcctggtgcc gaagcagttt gactacctgg tcaacagtat
    36121 gcgcgtcatg atggaccgcg ttcgtacgca agaacgtctg atcatgaagc tctgcgttga
    36181 gcagtgcaaa atgccgaaga aaaacttcat tactctgttt accggcaacg aaaccagcga
    36241 tacctggttc aacgcggcaa ttgcgatgaa caagccgtgg tcggaaaaac tgcacgatgt
    36301 ctctgaagaa gtgcatcgcg ccctgcagaa actgcagcag attgaagaag aaaccggcct
    36361 gaccatcgag caggttaaag atatcaaccg tcgtatgtcc atcggtgaag cgaaagcccg
    36421 ccgtgcgaag aaagagatgg ttgaagcgaa cttacgtctg gttatttcta tcgccaagaa
    36481 atacaccaac cgtggcttgc agttccttga cctgattcag gaaggcaaca tcggtctgat
    36541 gaaagcggtt gataaattcg aataccgccg tggttacaag ttctccacct acgcaacctg
    36601 gtggatccgt caggcgatca cccgctctat cgcggatcag gcgcgcacca tccgtattcc
    36661 ggtgcatatg attgagacta tcaacaaact caaccgtatt tctcgccaga tgctgcaaga
    36721 gatgggccgt gaaccgacgc cggaagaact ggctgaacgt atgctgatgc cggaagacaa
    36781 gatccgcaaa gtgctgaaga tcgccaaaga gccaatctcc atggaaacgc cgatcggtga
    36841 tgatgaagat tcgcatctgg gggatttcat cgaggatacc accctcgagc tgccgctgga
    36901 ttctgcgacc accgaaagcc tgcgtgcggc aacacacgac gtgctggctg gcctgaccgc
    36961 gcgtgaagcg aaagttctgc gtatgcgttt cggtatcgat atgaacaccg atcacacgct
    37021 ggaagaagtg ggtaaacagt tcgacgttac ccgtgaacgt atccgtcaga tcgaagcgaa
    37081 ggcgctgcgc aaactgcgtc acccgagccg ttctgaagtg ctgcgtagct tcctggacga
    37141 ttaatcggta ggccggatca ggcgttacgc cgcacccggc acctgtccct ttgcacaaac
    37201 gccacctttt cggtggcgtt ttttatcgcc cacgcaccac cagcgcctgg tccagctcgc
    37261 gatacgcttc aaccagtttc tccagtgaaa cgcgacttaa accgctggga tttggcagca
    37321 cccaaatctg cgtcgaacca atgctgagcg tttgtttccc ccactgtgca ccacgctggc
    37381 tgaatccctg ttcatatgct tgtttgccca gaatcgccaa cgcttgcggc tgataatctt
    37441 caattttttc aatcagctta cgcccacctg cgtgtagctc ttgctttgaa atttcattgg
    37501 cttgcaccgt tggcctgtct accagtttgg tgacgccaca acgataatcc agcagatgct
    37561 gtgcctcctg cggcttcaac tgatggtcgg taaacccggc ctgatatatc accttccaga
    37621 agcgatttgc cggatgagca aagggaaaac cagtcccggc ggatgaaagc ccagggttga
    37681 taccgcaaaa cacgacccgt aaccctggag ccaaaatatc ctcaaccata tttactcctt
    37741 tctaacatca ccaggaaaag tataacggat tgaaaataca ttgtttataa aaacagcagg
    37801 cgcgcggtaa tggctggatt gcgacacgga gttactttat aatccgctac catggcccct
    37861 tagctcagtg gttagagcag gcgactcata atcgcttggt cgctggttca agtccagcag
    37921 gggccaccag atatagcaaa ggctgacgag aaatcgtcag cctttttctt tttatatatc
    37981 agttactttg cgtgccagta ggccgctgca cgtacccgct gtgggtcata ctgttccgct
    38041 tcaaagcggc ggcttaaatt cttaacgact ttaccttcgc cggttatcca gatgaagtaa
    38101 tcatcggcag ggatttgcat ctgcgccaga cgcgcatcta ccgcctgctc atcatgtgcc
    38161 agccattcga tattaaaacc atcaaggtgc gcgagataat cctgacaggc gttatcccgc
    38221 acgctaacca gcgcactaac ttgcggttta acggcaagtt tgctcaacgt ttccaggcgg
    38281 cggcgcaatg cgggcattcc ggattcatcg cagacataca gctgatacgc gtaatcttcc
    38341 ggcaccacca gcgaaccgcg cggacctgcc accgtaagtt tatcgcccgg ttgcgcctgc
    38401 atcgcccagc cgctggcgac cccaccgtcg tgaataaaga aatcgatcgc cagttcatgg
    38461 cgtagttcgt catacagcgg cgtatagtca cgcgacggtg ggcgtggtcc ttccggccag
    38521 acgatgccct cttccgttac cgttggcggc acaaagtgag cgtcaggttg aggaaagaag
    38581 agtttgctgt gatcgtcaaa gccgcgcgat gtaaaaccgt ccagcgcctc gccgccaagg
    38641 acaatgcgct gaaaaccggc gctgatacgc tcaacgcgta gcacagtcaa ttcacggaag
    38701 cgcagatcat tgcgaacgcg ctgcgggtag cggggggagt tattcattgt tatcgccttc
    38761 gtgatggtaa tcagatatat ctaaataatt ttcgcaaatg ataatgattg ttaatcatga
    38821 taaatgcaag cgatttgtag aactgatatg tctatagtct gacaagacga accgcctctt
    38881 ctcaggcatc attactcaac gccggatgcg gcgtgaacgc cttatccggc ctacgtgtga
    38941 gatgagtcca catttaaaaa tcatttttat tcttgcaaga gtgctcatat cagatataaa
    39001 ttagatatat ctaattaagc aaaaggaggc tgatatgagc catcatcacg aagggtgttg
    39061 taaacatgaa ggccagccac gccatgaggg ctgctgcaaa ggtgagaagt cagaacacga
    39121 gcactgcgga cacggtcacc agcatgaaca cggtcaatgt tgcggtggtc gccacggtcg
    39181 cggcggtggt cgtcggcaac gtttctttgg tcacggtgaa ttgcgtctgg tgattctgga
    39241 tattctctcg cgcgatgaca gccacggtta cgaattgatt aaagcgattg agaatctaac
    39301 ccaagggaat tacaccccaa gcccgggcgt catctacccg acgctggatt ttctgcagga
    39361 gcagtcgctg attaccatcc gcgaagagga aggtggtaag aagcagattg cgctgaccga
    39421 acaaggcgcg cagtggctgg aagaaaaccg cgaacaggtg gagatgattg aagaacgcat
    39481 caaagcgcgt tgcgttggcg ctgcgctgcg ccagaacccg caaatgaagc gggcgctaga
    39541 taattttaaa gcggtgctga atttacgcgt caaccagagt gatatcactg atgcacaaat
    39601 aaaaaagatc attgcggtga tcgaccgcgc cgcttttgat attacgcaac tggattaatc
    39661 gccgcatccg ccagtggcgc ggtgcaattg ccggatgcga cgcttgacgc accttatccg
    39721 gcctacaccc gctacacacc ccgtaggcct gataagatgc gccagcatcg catcaggcat
    39781 cgtgtatcaa ataccggatc cggcataccg attaatgcag caccgtcacc gcgtcttcca
    39841 gtcggctggc gcggtgtttc accatcgccg acacctgcgc actctcttcc accagctcgg
    39901 catttttctg ggtgatgagg ttaagctcat ccactgcacg ggtcaggctg gaaagaccat
    39961 cggcctgttc cagcgttgaa tggctaatct gggcaatcaa ctgggtgacg tttttcacct
    40021 gtgccacaat atcttccatc gtacgtccgg cggcgtgtac ctgctgcgaa ccggattgca
    40081 ccttatcagc actggcatca atcagcttgc gaatatcgtt ggcagcattg gcgctgcggc
    40141 tggctaaatg acgcacttcc cccgccacca ccgcaaaacc tttgccctgt tcgccggcac
    40201 gcgccgcttc caccgccgca ttcagggcca gaatattggt ctgaaacgca atatcgttaa
    40261 tcagcgaagt aatggtgcca atgcgctggg tactgtcggc gatatcgtcc attgtcttga
    40321 tcaccgtggt catcgcctcc ccgccctgca ctgccgcatt actggcggtg attgacagtt
    40381 tatcggcagc cgacgccgtg gcagagttct gtttcaccga cgccgccatt tggttcatgg
    40441 tcgccaccgt ttgctgaacg ttatcaactg tctgctgggt atgttcgttc agttcatcgg
    40501 tgcctttcgc cagcgtctca ctgccgtttc tgacactgga cacctggctt gagacatcgt
    40561 taattaacca acggcacatc aggccaagct gccctaccgc gcgtaatgtc agccccagct
    40621 catcgctgcg attcagatgc tcaacactat tacgctctcc ggtcgccacc ttcagtgcct
    40681 gacgggcgac attttctatc gggcggacaa tctgccattc aaaacaggcg cttgccaaca
    40741 ataccactaa cgcacagagg atatacgtca ccaccggggc agcaacaaac caaagcatgg
    40801 ccgccagcaa gataaacatc agggtcatca ctccacgcgc ccgccagcga agcggtaatg
    40861 aaggcagttt acccagccag cctttacgca ccaccaggcc tttatgaata cgcttaccgg
    40921 tacgtccggc gttcagcgct ttgtacagcg gctccaccgc cgcgatctct tcatccgtcg
    40981 cccgggtacg aatcgacata tagccactga tttttccctc gcgcaccatc ggtaccgcat
    41041 tggcccgcac ccaataatgg tcaccatttt tgcggcgatt tttcacgatg ccgctccagg
    41101 gctccccttt tttcagggtg aaccacatat ccgcaaacgc cgcttttggc atatccgggt
    41161 gacgcaccat gttgtgcggc tgcccttgta actcttgcaa ggtaaagccg ctcacctgca
    41221 caaaagtgtc attagcatga gtgatatagc tttgcagatc ggtagtggac atcagagtgg
    41281 tatcgtccgc cagcggggta ttttgctggg tgacatacgg atgagaagac atgttcgcgt
    41341 cctgtgcagg ttatatggtt gttaacttct tgtcagagtt tatgtcggcc ccgctgcggt
    41401 tatctttaac cgattaattt gatttagatc gcaatttgcg attaaagcac aaatctaatt
    41461 cgttgattta aaatactttc actctgttac tatacgaaaa cgttaattat cttgcccaaa
    41521 aatcaggcag ttaatgccct gaaagcgtgc atttgcgcag caattatcaa atccataccc
    41581 gacaaaaacc gtgcaaaata acaacagatg ttaacaaata gcattaaata ttgcgcaaat
    41641 gataaccaca tttgtgttta tcccgatttt cgcgatcgca gccggagtgg cgcaatccct
    41701 gcaatactta aatcggtatc atgtgatacg cgagcctccg gagcatattt tgaacaggtt
    41761 accttcgagc gcatcggctt tagcgtgcag cgcccacgcc ctgaatctca ttgaaaagcg
    41821 aacgctggat catgaggaga tgaaagcact taaccgagag gtgattgaat acttcaaaga
    41881 gcatgtcaat ccggggtttt tagagtatcg caaatctgtt accgccggcg gggattacgg
    41941 agccgtagag tggcaagcgg gaggtttaaa tacgcttgtc gacacccagg gacaggagtt
    42001 tatcgactgc ctgggaggtt ttggaatttt caacgtgggg caccgtaatc cagttgtggt
    42061 ttccgccgta cagaatcaac ttgcgaaaca accactgcac agccaggagc tactcgatcc
    42121 gttacgggct atgctggcga aaacccttgc tgcgctaacg ccaggtaaac tgaaatacag
    42181 cttcttctgt aatagcggca ccgagtccgt tgaagcggcg ctgaagctgg cgaaggctta
    42241 tcagtcgccg cgcggcaaat ttacttttat tgccaccagc ggcgcgttcc acggtaaatc
    42301 acttggcgcg ctgtcggcca cggcgaaatc gaccttccgc aaaccgttta tgccactgct
    42361 gccagggttc cgtcatgtgc cgtttggcaa tatcgaagcc atgcgcacgg ctcttaatga
    42421 gtgcaaaaaa accggtgatg atgtggccgc ggtgatcctc gaaccgattc agggcgaagg
    42481 tggcgtaatt ctgccgccgc cgggctatct caccgccgtg cgtaagctgt gcgatgagtt
    42541 cggcgcactg atgatcctcg atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt
    42601 cgcctgcgaa catgagaacg tacagccgga catcctgtgc ctggcaaaag ccctcggcgg
    42661 cggtgtgatg ccgattggcg caaccatcgc cactgaagag gtgttctcag ttctgttcga
    42721 caacccgttc ctgcatacca ccacctttgg cggcaacccg ctggcctgtg cggcggcgct
    42781 ggcgaccatc aatgtgttgc tggagcagaa cttaccggct caggctgagc aaaaaggcga
    42841 tatgttgctg gacggtttcc gtcaactggc gcgggaatat cccgatctgg tacaggaagc
    42901 gcgtggtaaa gggatgttaa tggcgattga gtttgttgat aacgaaatcg gctataactt
    42961 tgccagcgag atgttccgcc agcgcgtact ggtggccgga acgctcaata acgccaaaac
    43021 gatccgcatt gaaccgccac tgacactgac cattgaacag tgtgaactcg tgatcaaagc
    43081 ggcgcgtaag gcgctggcgg ccatgcgagt aagtgtcgaa gaagcgtagt atcaaatcgg
    43141 atggcaatac gacgtcgcca tccgatcttt ttatacgata cgtacgcccg caggcatcat
    43201 ccgctccggc gttaacagca cgctttcact gccgtcatcg gtttccgcac acaacagcat
    43261 gcattccgac gtttcaccac gcattttcgc tttttgcaga ttgcataaga ccaccaccgt
    43321 ttttcccatc aactcttctt cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt
    43381 tttttccccc acatcaacct gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac
    43441 ttccacaatc tttccgacgc gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc
    43501 cattgcttcc tcccttcgag taaaatttta ctaaactata gcaaaagttt ttctcaatcc
    43561 tgtaggctaa aaatggagaa tgcaggcgtg atcacattcg tgagccgctg tgttaccgtt
    43621 acagcgtcaa agaaacgcgc tttatttact gaaaacaggt gacccgataa gcacttcctc
    43681 tacaatgggg gcgcacatca gggaaagtaa aaaaggtaaa catggcaaca ctaaaagaca
    43741 tcgcaatcga agctggcgta tccctggcga cagtatccag ggtcttaaat gacgatccga
    43801 cattgaatgt gaaagaagag acgaaacatc gcattctcga gatcgccgaa aagctggagt
    43861 acaagaccag tagcgcccgt aaactacaga caggcgcagt caaccaacac catattctgg
    43921 ctatctacag ctaccagcag gagctggaga tcaacgatcc ttactatctg gcgatccgcc
    43981 acggcattga aactcagtgc gaaaagctgg cgatcgaact gaccaactgt tatgaacaca
    44041 gcggattacc agacattaaa aacgtcaccg gtattttaat tgtcggcaaa cccacgcccg
    44101 ccctgcgcgc cgctgccagc gcgttgaccg acaatatctg ttttatcgac tttcacgaac
    44161 ccggcagcgg ttacgatgcg gtggatatcg atctggcacg catcagtaaa gaaatcatcg
    44221 acttctatat caaccagggc gttaatcgta ttggttttat tggcggtgaa gatgagcctg
    44281 gcaaggcgga tattcgtgag gtcgcctttg cggaatatgg ccgactgaaa caagtggtac
    44341 gcgaagagga tatctggcgc ggcggttttt ccagttcgtc gggttatgaa ctggcaaaac
    44401 aaatgctggc gcgggaagac tatccgaagg cactgtttgt tgcttccgat tccattgcta
    44461 tcggcgtact gcgggcgatt catgaacgtg gtctgaacat cccacaggat atttcgctta
    44521 tcagcgttaa cgatatcccc accgcgcgat ttacctttcc gccgctctcc accgtgcgca
    44581 tccattccga aatgatggga agtcagggcg taaacctggt gtatgaaaaa gcccgcgatg
    44641 gtcgcgcgct gccgctgtta gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc
    44701 gttaaatccc cttacacact gtccggcaat cgtttttgcc gggcagtgct gccgtttatt
    44761 ttcgtgatcc agttaaagta aatgcattta cccgctactt tttagtaaaa attttactaa
    44821 actccccagc aattacacaa actaccatca ccatgaatgg ttccgatttc tctctaccgg
    44881 gaggccctat gaatcgctgg gaaaacattc agctcaccca cgaaaaccga cttgcgccgc
    44941 gtgcgtactt tttttcatat gattctgttg cgcaagcgcg tacctttgcc cgcgaaacga
    45001 gcagcctgtt tctgccctta agcggtcagt ggaatttcca cttttttgac catccgctgc
    45061 aagtgccaga agccttcacc tctgagttaa tggctgactg ggggcatatt accgtccccg
    45121 ccatgtggca aatggaaggt cacggcaaac tgcaatatac cgacgaaggt tttccgttcc
    45181 ccatcgatgt gccgtttgtt cccagcgata acccaaccgg tgcctatcaa cgtattttca
    45241 ccctcagtga cggctggcag ggtaaacaga cgctgattaa atttgacggc gtcgaaacct
    45301 attttgaagt ctacgttaac ggtcagtatg tgggtttcag caagggcagt cgcctgaccg
    45361 cagagtttga catcagcgcg atggttaaaa ccggcgacaa cctgttgtgt gtgcgcgtga
    45421 tgcagtgggc ggactctacc tacgtggaag accaggatat gtggtggtca gcggggatct
    45481 tccgcgatgt ttatctggtc ggaaaacacc taacgcatat taacgatttc actgtgcgta
    45541 ccgactttga cgaagcctat tgcgatgcca cgctttcctg cgaagtggtg ctggaaaatc
    45601 tcgccgcctc ccctgtcgtc acgacgctgg aatataccct gtttgatggc gaacgcgtgg
    45661 tgcacagcag cgccattgat catttggcaa ttgaaaaact gaccagcgcc agctttgctt
    45721 ttactgtcga acagccgcag caatggtcag cagaatcccc ttatctttac catctggtca
    45781 tgacgctgaa agacgccaac ggcaacgttc tggaagtggt gccacaacgc gttggcttcc
    45841 gtgatatcaa agtgcgcgac ggtctgttct ggatcaataa ccgttatgtg atgctgcacg
    45901 gcgtcaaccg tcacgacaac gatcatcgca aaggccgcgc cgttggaatg gatcgcgtcg
    45961 agaaagatct ccagttgatg aagcagcaca acatcaactc cgtgcgtacc gctcactacc
    46021 cgaacgatcc gcgtttttac gaactgtgtg atatctacgg cctgtttgtg atggcggaaa
    46081 ccgacgtcga atcgcacggc tttgctaatg tcggcgatat cagccgtatt accgacgatc
    46141 cgcagtggga aaaagtctac gtcgagcgca ttgttcgcca tattcacgcg cagaaaaacc
    46201 atccgtcgat catcatctgg tcgctgggca atgaatccgg ctatggctgt aacatccgcg
    46261 cgatgtacca cgcagcgaag gcgctggatg acacgcgact ggtgcattac gaagaagatc
    46321 gcgatgctga agtggtcgat attatttcca ccatgtacac ccgcgtgccg ctgatgaatg
    46381 agtttggtga atacccgcat ccgaagccgc gcatcatctg tgaatatgct catgcgatgg
    46441 ggaacggacc gggcgggctg acggagtacc agaacgtctt ctataagcac gattgtattc
    46501 agggacatta tgtttgggag tggtgcgacc acgggatcca ggcgcaggat gacaacggca
    46561 atgtctggta taaattcggc ggcgactacg gcgactatcc caacaactat aacttctgtc
    46621 ttgatggttt gatctattcc gatcagacgc cgggaccagg cctgaaagag tacaaacagg
    46681 ttatcgcgcc ggtaaaaatc cacgcgctgg atctgactcg cggcgagctg aaagtcgaaa
    46741 ataaactgtg gtttaccacg ctggatgact acaccctgca cgcagaggtg cgcgccgaag
    46801 gtgaaacgct cgcgacgcaa cagattaaac tgcgcgacgt tgcgccgaac agcgaagccc
    46861 ccttgcagat cacgctgccg cagctggacg cccgcgaagc gttcctcaac attacggtga
    46921 ccaaagattc ccgcacccgc tacagcgaag ccgggcattc tatcgccact tatcagttcc
    46981 cgctgaagga aaacaccgcg cagcctgtac ctttcgcacc gaataacgcc cgtccgctga
    47041 cgctggaaga cgatcgtttg agctgtactg ttcgcggcta caactttgcg atcaccttct
    47101 caaaaacgag tggtaaaccg acatcctggc aggtaaatgg cgaatcgctg ctgacccgcg
    47161 agccaaagat caacttcttc aagccaatga tcgacaacca caagcaggag tacgaagggt
    47221 tgtggcagcc gaatcatttg cagatcatgc aggaacatct gcgcgacttt gccgtagaac
    47281 agagcgatgg tgaagtgttg atcatcagcc gcacggttat agcaccgccg gtgtttgact
    47341 tcgggatgcg ctgtacctac atctggcgca tcgctgcaga tggccaggtt aacgtggcgc
    47401 tttccggcga gcgttacggc gactatccgc acatcattcc gtgcatcggt ttcaccatgg
    47461 ggattaacgg cgaatacgat caggtggcgt attacggtcg tggaccgggc gaaaactacg
    47521 ccgacagcca gcaggctaac atcatcgata tctggcgcag caccgtcgat gccatattcg
    47581 agaactatcc cttcccgcag aacaacggca accgtcagca tgtccgctgg acggcactga
    47641 ctaaccgcca cggcaacggt ctgctggtgg ttccgcagcg cccaattaac ttcagcgcct
    47701 ggcactatac ccaggaaaac atccacgctg cccagcactg taacgagctg cagcgcagtg
    47761 atgacatcac cctgaatctc gaccaccagc tgcttggcct cggctccaat tcctggggca
    47821 gcgaggtgct ggactcctgg cgcgtctggt tccgtgactt cagctacggc tttacgttgc
    47881 tgccggtttc tggcggagaa gctaccgcgc aaagcctggc gtcgtatgag ttcggcgcag
    47941 ggttcttttc cacaaatttg cacagcgaga ataagcaatg aggatcatcg ataacttaga
    48001 acagttccgc cagatttacg cctctggtaa gaagtggcaa cgctgcgttg aagcgattga
    48061 aaatatcgac aacattcagc ctggcgtcgc ccactccatc ggtgactcat tgacttaccg
    48121 cgtggagaca gactccgcga ccgatgcgct atttaccggg catcgacgct attttgaagt
    48181 gcattactac ctgcaagggc agcaaaaaat tgaatatgcg ccgaaagaga cattacaggt
    48241 cgtggaatat tatcgtgatg aaactgaccg tgaatattta aaaggctgcg gagaaaccgt
    48301 tgaggtcgac gaagggcaaa tcgttatttg cgatatccat gaagcatatc ggtttatctg
    48361 caataacgcg gtcaaaaaag tagttctcaa agtcaccatc gaagatggtt atttccataa
    48421 caaataacaa ctacggcggc aaaaggagtt tgccgccacc gctaccctac tcattttcgg
    48481 agatgtgtta tgtctgatac caaacgtaat acaatcggca aattcggctt gctctcgctg
    48541 acttttgccg ccgtttacag ctttaacaac gttatcaata ataatattga gcttggactg
    48601 gcctcggcac cgatgttttt cctcgcgacg attttttatt ttattccctt ctgtctgatc
    48661 atcgcagaat ttgtttcgtt aaataaaaac tcagaagccg gtgtctacgc gtgggtaaaa
    48721 agttcgctgg gcggacgttg ggcatttatt actgcctata cctactggtt cgtaaacctg
    48781 ttctttttca cctcgctgtt gccgcgcgtt attgcttatg cttcgtatgc cttcctcggc
    48841 tatgaatata ttatgacgcc ggttgccacc accattatca gtatggtgct gttcgccttc
    48901 tccacctggg tttccaccaa cggggcgaaa atgttggggc caattacctc cgtcacttca
    48961 acgctgatgc tgctgttaac gctctcctac attttactgg caggtacggc gctggttggc
    49021 ggcgtacagc ctgctgaccc catcaccgtt gacgcgatga tcccgaactt caactgggcg
    49081 ttcctcggcg ttaccacctg gatctttatg gccgcaggtg gcgcggagtc cgtcgctgtg
    49141 tacgttaacg acgtcaaagg cggttcgaaa tcgttcgtta aagtgatcat cctcgccggg
    49201 atttttatcg gtgtactata ttccgtctcc tcggtgctga ttaacgtctt cgtcagcagc
    49261 aaagagttga aatttactgg cggatcggtg caggtattcc acggcatggc ggcgtatttt
    49321 ggtctgccgg aagcgttgat gaatcgcttt gtcggtctgg tgtcctttac cgcaatgttc
    49381 ggttccctgc tgatgtggac cgcaacgccg gtgaaaattt tcttctccga aatcccggaa
    49441 ggcatctttg gtaagaaaac cgtcgaactt aacgaaaacg gcgttccggc gcgcgcagcg
    49501 tggatccagt tcctgatcgt catcccgctg atgattatcc cgatgctcgg ttccaatact
    49561 gtgcaggatc tgatgaatac tattattaat atgaccgccg cagcgtccat gcttccgccg
    49621 ttattcatca tgctggctta cctgaattta cgcgccaaat tagatcacct gccacgcgat
    49681 ttccgtatgg gctcccgccg caccggtatt atcgttgttt caatgctgat tgcgatattt
    49741 gccgtagggt ttgtcgcttc gacattcccg actggcgcga atattctgac catcattttt
    49801 tataacgtcg gcggtattgt tatattcctt ggctttgcgt ggtggaaata cagtaaatat
    49861 ataaagggat taacggctga agagcgccat attgaagcga cgccagccag caatgttgat
    49921 taaacagact aataaaaaat gagcaggcat tttccctctt cctggatgag gagagggaaa
    49981 atagtttctg cctttatatt tttataatta caacgataaa aggctgtact ttttctttag
    50041 ctcatggatt aacacaatga aattaatcac tgcaccatgc agagcattac ttgctctgcc
    50101 gttttgctac gccttttctg cggcaggaga agaagcacgt ccggcagaac atgacgacac
    50161 aaaaaccccc gcaattacct cgacatcttc tccttcattt cgtttttacg gcgaattagg
    50221 ggttggtgga tatatggatt tagagggtga gaataaacat aaatacagcg acggcaccta
    50281 tattgaaggt ggcctggaga tgaagtacgg ctcctggttc ggcctgattt acggcgaagg
    50341 ctggaccgtg caggccgacc acgacggcaa tgcctgggtg cctgaccata gctggggcgg
    50401 tttcgaaggt gggattaacc gtttctatgg cggttatcga accaatgatg gcaccgaaat
    50461 catgctcagt ctgcgtcagg attcctcgct ggatgacctg caatggtggg gcgacttcac
    50521 acccgatctg ggctacgtca ttcccaatac ccgcgacatt atgaccgcgc tgaaggtgca
    50581 gaacttaagc ggcaactttc gttatagcgt caccgccaca cctgccggac atcatgacga
    50641 aagcaaagca tggctacatt ttggcaagta cgatcgctac gacgacaaat acacctatcc
    50701 ggcaatgatg aacggttaca tccagtatga ccttgccgaa ggcatcacct ggatgaatgg
    50761 tctggaaatt accgacggca caggacaact ctatctcacg ggactgctta cccctaactt
    50821 tgccgctcgc gcctggcacc ataccggacg tgccgacggg ctggacgtac cgggaagtga
    50881 aagcgggatg atggtgagcg ccatgtatga agcgttaaag ggcgtttatc tctccaccgc
    50941 ttacacctac gccaaacatc gccctgacca cgctgacgat gaaaccacct ctttcatgca
    51001 gtttggtatc tggtacgaat atggcggcgg acgtttcgca acggcttttg atagccgctt
    51061 ctacatgaaa aatgcctcta acgatcccag cgaccaaatc ttcctgatgc aatatttcta
    51121 ctggtaataa ggactgtaat ttcatgaaaa tcaaaactat tttaacgcca gtaacctgcg
    51181 ctctgctgat aagtttttcc gcccatgcca ctaacgccga caattataaa aacgtgatta
    51241 accgtactgg tgcgccgcag tacatgaagg attacgatta cgacgatcac cagcgtttta
    51301 atccgttttt cgatctcgga gcctggcatg gtcatctgtt gccagacggc ccgaacacca
    51361 tgggcggctt tccgggcgtt gcgctgctga cggaagaata catcaacttt atggccagca
    51421 atttcgaccg cctgaccgtc tggcaggacg gcaagaaagt caacttcacg ctggaggcat
    51481 acaatattcc cggcgcgctg gtgcaaaaac tgacagcaaa agatgtgcag gtcgaaatga
    51541 ctctgcgctt cgccacgccg cgcacgtcac tactggaaac taaaatcatc agcaataaac
    51601 cgctggatct ggtgtgggat ggcgaactgc tggaaaaact ggaagcgaaa gaagggaaac
    51661 cgctttccga taaaaccatt gctggcgaat accctgacta ccagcgcaaa atcagcgcca
    51721 cccgtgatgg cctgaaagtc acctttggca aagtgcgcgc cacctgggat ctgctgacct
    51781 ccggtgaatc agaatatcag gtgcataaat ccctgccggt gcagactgaa atcaacggca
    51841 atcgctttac cagtaaggcg catatcaacg gttcgacgac gctctatacc acctattccc
    51901 atctgctgac ctctcaggaa gttagcaaag agcaaatgca gatccgcgat attctggcgc
    51961 gtccggcgtt ttatctcacc gcctcgcagc aacgctggga agagtatctg aagaaagggt
    52021 taaccaatcc ggatgcgacg ccggaacaga cacgcgtcgc ggtgaaagcg atcgaaacgc
    52081 tcaacggtaa ctggcgctcg cctgggggtg cggtgaaata taacaccgtc acgccgtcgg
    52141 tgaccgggcg ctggttctcc ggcaatcaga cctggccgtg ggatacctgg aagcaggcgt
    52201 ttgcgatggc gcatttcaat ccggacatcg ccaaagagaa tatccgcgcg gtcttctcct
    52261 ggcagatcca gcctggcgac agcgtgcgtc cgcaggatgt gggctttgtc cccgacctga
    52321 tagcgtggaa tcttagcccc gagcgtggtg gcgatggtgg caactggaac gaacgaaata
    52381 ccaaacccag ccttgccgcc tggtcggtga tggaagtgta caacgtcacc caggataaaa
    52441 cctgggtggc agagatgtac ccgaaactgg tggcctatca cgactggtgg ttacgtaacc
    52501 gcgatcacaa cggcaacggc gtgccggaat atggcgcgac ccgcgacaaa gcccacaaca
    52561 ctgagagcgg cgagatgctg tttacggtga agaaaggcaa caaagaagag acgcagtctg
    52621 ggctgaacaa ctacgcccgc gtggtggaga aaggccagta cgacagtctg gaaattccgg
    52681 cacaggttgc tgcatcgtgg gaatcggggc gtgatgatgc cgccgtcttt gggtttatcg
    52741 acaaagaaca gctggataaa tatgtcgcaa gcggcggcaa acgtagcgac tggacagtga
    52801 aattcgccga aaaccgcagt caggacggaa cgttgctggg gtactcgcta ttgcaggagt
    52861 cggtggatca ggccagctat atgtacagcg ataaccatta tctggcggag atggcgacga
    52921 ttctcggtaa accggaagag gctaagcgct atcgccagtt ggcacagcag ctcgcggact
    52981 acatcaacac ctgtatgttc gacccggcta cgcagtactt ctatgacgtg cgtattgaag
    53041 ataaaccgct ggcgaacggc tgcgcgggca aaccgattgt tgagcgcggt aaagggccgg
    53101 aaggctggtc gccgctgttt aacggtgcgg caacgcaggc caatgccgac gcggtggtga
    53161 aggtgatgct cgatcctaaa gagttcaata cctttgttcc gctgggaacg gcggcgttaa
    53221 ccaatccggc ctttggcgct gatatctact ggcgcgggcg cgtatgggtg gatcagttct
    53281 ggtttggtct gaaagggatg gagcgttacg gttatcgcga tgatgccctg aaactggcgg
    53341 atacgttctt ccggcacgcc aaagggttaa ccgccgatgg cccaattcag gaaaattaca
    53401 acccgctgac aggcgcacag caaggcgcac caaatttctc ctggagtgcc gcacattttt
    53461 atatgttgta taacgatttt ttccgtaagc aataatttgc aacggctggc ggatgcggcg
    53521 tgaacgcctt atccgcccta catattcgtt cccgtaggct ggataagacg cgacaagcgt
    53581 cgcatccggc atctgcacct cgccaccggc ggatgctgcg tgaacgcctt atccgcccta
    53641 catgttcgtt cccgtaggct ggataagacg cgacaagcgt cgcatccggc atctgcacct
    53701 cgccactggc ggatgcggcg tgaacgcctt atccgcccta catgttcgtt cccgtaggct
    53761 ggataagacc cgacaagcgt cgcatccggc gtctgcaccg cgccactggc ggatgcggcg
    53821 tgaacgcctt atccgcccta catgtgcaat cccccaggtc ggatagggcc cggcaagcgt
    53881 cgcatccggc gttaccaccg ggcgtattct ttttgaatcc catcacaaac cccgcactcc
    53941 ccttttccct tttctccggc gacggctaaa ttagaactca tccgaccaca taacaattat
    54001 tttacatact ggacaatttt atgagctacc cgtcgctgtt cgccccgctg gatttaggtt
    54061 ttaccacgtt aaaaaaccgc gtgttgatgg gttcaatgca caccgggctg gaggaatacc
    54121 cggacggtgc tgaacggctg gcagcgtttt atgccgaacg cgcccgtcac ggcgtggcgc
    54181 tgattgttag cggcggcatt gcaccagatt taacaggcgt tggcatggaa ggcggcgcaa
    54241 tgctcaatga cgccagccag atcccacacc atcgcaccat taccgaagcg gtacatcagg
    54301 aaggcggcaa aatagccctg caaattttgc ataccgggcg ctacagctac caaccgcatc
    54361 tggtcgcacc gtccgcattg caggccccca tcaaccgttt cgttcctcat gaactcaccc
    54421 atgaagagat cctgcaactg atcgacgatt tcgcccgctg cgcgcaactg gcacgggagg
    54481 caggatacga cggtgtagag gtaatgggtt ccgaagggta tttgatcaac gaatttctga
    54541 cgctgcgcac caatcagcgc agtgaccagt ggggcggcga ttaccacaac cggatgcggt
    54601 ttgccgttga agtggtgagt gcggtacgcg aacgcgttgg caacgacttc attattatct
    54661 accgactgtc gatgctcgac ctggtcgaag gcggcgggac ttttgccgaa acggtagagc
    54721 tggcgcaggc cattgaagcg gcgggcgcga ccattatcaa caccggcatt ggctggcatg
    54781 aagcgcgtat tccgaccatc gccacgcccg tgccacgcgg cgcatttagc tgggtcacgc
    54841 gcaaactgaa aggtcacgtc tcgctgccgc tggtgaccac caaccggatt aacgatccgc
    54901 aggttgccga cgatattctc tcgcgcggcg atgccgatat ggtatcgatg gcgcgaccgt
    54961 ttcttgcgga tgcggaactg ctgtcaaaag cgcaatcagg gcgagccgat gagatcaaca
    55021 cctgcattgg ctgcaaccag gcgtgtctcg atcagatctt cgttggcaag gtcacctcgt
    55081 gtctggtgaa tcctcgcgcc tgccatgaaa ccaaaatgcc gatccttccc gccatgcaga
    55141 aaaaaaatct ggcggtggtc ggtgcgggac ctgctgggct ggcgtttgcc attaacgcgg
    55201 cggcgcgtgg tcatcaggta acgttgtttg acgcgcacag tgagatcggc gggcagttta
    55261 atatcgccaa acagatcccc ggcaaagagg agttttacga aacgctgcgc tattaccgcc
    55321 ggatgatcga agtgacgggc gtgacgctaa aactcaatca caccgtgacg gcggatcagt
    55381 tacaggcgtt cgatgaaacg atcctcgcca gtgggatcgt gccgcgcact ccgcccatcg
    55441 acgggatcga tcatccgaag gtattgagtt atctcgatgt actgcgcgac aaagcgccgg
    55501 tgggcaacaa agttgccatc atcggttgtg gcgggattgg ttttgatacg gcgatgtatt
    55561 taagtcagcc gggcgaatcc accagccaga atatcgccgg gttctgtaat gaatggggga
    55621 tcgacagcag cctacaacag gctggtggct taagcccgca gggaatgcag atcccgcgta
    55681 gcccacggca gattgtgatg ctccagcgca aagccagcaa accgggacag gggttaggca
    55741 aaaccaccgg ctggatccat cgcaccaccc tgctctcgcg gggtgtgaaa atgatcccag
    55801 gcgtaagtta tcagaagatt gacgatgacg ggctgcatgt ggtgatcaac ggcgaaacgc
    55861 aggtattagc agtggacaat gtggtgatct gcgcagggca agagccaaac cgcgcattgg
    55921 cgcagccgct gattgatagc gggaaaacgg tgcatttaat tggcggctgc gatgtggcta
    55981 tggagctgga cgcacgacgg gcaattgccc agggaacacg gctggcgctg gagatttaaa
    56041 tcgtgcatt
//
LOCUS       DAZKIF010000011        55550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZKIF010000011 DAZKIF010000000
VERSION     DAZKIF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.011"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            311..1627
                     /gene="fucP"
                     /locus_tag="ACHOTW_000749"
     CDS             311..1627
                     /gene="fucP"
                     /locus_tag="ACHOTW_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP9842913.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1660..3435
                     /gene="fucI"
                     /locus_tag="ACHOTW_000750"
     CDS             1660..3435
                     /gene="fucI"
                     /locus_tag="ACHOTW_000750"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP9842914.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3544..4962
                     /gene="fucK"
                     /locus_tag="ACHOTW_000751"
     CDS             3544..4962
                     /gene="fucK"
                     /locus_tag="ACHOTW_000751"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP9842915.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQFRYFYPQTEPEFIEEV"
     gene            4964..5386
                     /gene="fucU"
                     /locus_tag="ACHOTW_000752"
     CDS             4964..5386
                     /gene="fucU"
                     /locus_tag="ACHOTW_000752"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP9842916.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5444..6175
                     /gene="fucR"
                     /locus_tag="ACHOTW_000753"
     CDS             5444..6175
                     /gene="fucR"
                     /locus_tag="ACHOTW_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP9842917.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(6219..7319)
                     /gene="rlmM"
                     /locus_tag="ACHOTW_000754"
     CDS             complement(6219..7319)
                     /gene="rlmM"
                     /locus_tag="ACHOTW_000754"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP9842918.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7312..7707)
                     /gene="ygdD"
                     /locus_tag="ACHOTW_000755"
     CDS             complement(7312..7707)
                     /gene="ygdD"
                     /locus_tag="ACHOTW_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP9842919.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7726..8643)
                     /gene="gcvA"
                     /locus_tag="ACHOTW_000756"
     CDS             complement(7726..8643)
                     /gene="gcvA"
                     /locus_tag="ACHOTW_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP9842920.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(8994..9221)
                     /gene="ygdI"
                     /locus_tag="ACHOTW_000757"
     CDS             complement(8994..9221)
                     /gene="ygdI"
                     /locus_tag="ACHOTW_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP9842921.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9413..10618
                     /gene="csdA"
                     /locus_tag="ACHOTW_000758"
     CDS             9413..10618
                     /gene="csdA"
                     /locus_tag="ACHOTW_000758"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP9842922.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10618..11061
                     /gene="csdE"
                     /locus_tag="ACHOTW_000759"
     CDS             10618..11061
                     /gene="csdE"
                     /locus_tag="ACHOTW_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP9842923.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(11112..11918)
                     /gene="tcdA"
                     /locus_tag="ACHOTW_000760"
     CDS             complement(11112..11918)
                     /gene="tcdA"
                     /locus_tag="ACHOTW_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP9842924.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12157..13254)
                     /gene="mltA"
                     /locus_tag="ACHOTW_000761"
     CDS             complement(12157..13254)
                     /gene="mltA"
                     /locus_tag="ACHOTW_000761"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP9842925.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13463..13539
                     /locus_tag="ACHOTW_000762"
     tRNA            13463..13539
                     /locus_tag="ACHOTW_000762"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13497..13499,aa:Met,seq:cat)
     gene            complement(13613..14866)
                     /gene="amiC"
                     /locus_tag="ACHOTW_000763"
     CDS             complement(13613..14866)
                     /gene="amiC"
                     /locus_tag="ACHOTW_000763"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP9842926.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15098..16429
                     /gene="argA"
                     /locus_tag="ACHOTW_000764"
     CDS             15098..16429
                     /gene="argA"
                     /locus_tag="ACHOTW_000764"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP9842927.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16491..18317)
                     /gene="recD"
                     /locus_tag="ACHOTW_000765"
     CDS             complement(16491..18317)
                     /gene="recD"
                     /locus_tag="ACHOTW_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP9842928.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18317..21859)
                     /gene="recB"
                     /locus_tag="ACHOTW_000766"
     CDS             complement(18317..21859)
                     /gene="recB"
                     /locus_tag="ACHOTW_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP9842929.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(21852..24740)
                     /gene="ptrA"
                     /locus_tag="ACHOTW_000767"
     CDS             complement(21852..24740)
                     /gene="ptrA"
                     /locus_tag="ACHOTW_000767"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP9842930.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24916..28284)
                     /gene="recC"
                     /locus_tag="ACHOTW_000768"
     CDS             complement(24916..28284)
                     /gene="recC"
                     /locus_tag="ACHOTW_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP9842931.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDHELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(28297..28620)
                     /gene="ppdC"
                     /locus_tag="ACHOTW_000769"
     CDS             complement(28297..28620)
                     /gene="ppdC"
                     /locus_tag="ACHOTW_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP9842932.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(28605..29012)
                     /gene="ygdB"
                     /locus_tag="ACHOTW_000770"
     CDS             complement(28605..29012)
                     /gene="ygdB"
                     /locus_tag="ACHOTW_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP9842933.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(29009..29572)
                     /gene="ppdB"
                     /locus_tag="ACHOTW_000771"
     CDS             complement(29009..29572)
                     /gene="ppdB"
                     /locus_tag="ACHOTW_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9842934.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(29563..30033)
                     /gene="ppdA"
                     /locus_tag="ACHOTW_000772"
     CDS             complement(29563..30033)
                     /gene="ppdA"
                     /locus_tag="ACHOTW_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9842935.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30217..31011)
                     /gene="thyA"
                     /locus_tag="ACHOTW_000773"
     CDS             complement(30217..31011)
                     /gene="thyA"
                     /locus_tag="ACHOTW_000773"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9842936.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(31018..31893)
                     /gene="lgt"
                     /locus_tag="ACHOTW_000774"
     CDS             complement(31018..31893)
                     /gene="lgt"
                     /locus_tag="ACHOTW_000774"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9842937.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(32044..34290)
                     /gene="ptsP"
                     /locus_tag="ACHOTW_000775"
     CDS             complement(32044..34290)
                     /gene="ptsP"
                     /locus_tag="ACHOTW_000775"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9842938.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34303..34833)
                     /gene="rppH"
                     /locus_tag="ACHOTW_000776"
     CDS             complement(34303..34833)
                     /gene="rppH"
                     /locus_tag="ACHOTW_000776"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP9842939.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(35186..35332)
                     /gene="ygdT"
                     /locus_tag="ACHOTW_000777"
     CDS             complement(35186..35332)
                     /gene="ygdT"
                     /locus_tag="ACHOTW_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP9842940.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            35518..36207
                     /gene="mutH"
                     /locus_tag="ACHOTW_000778"
     CDS             35518..36207
                     /gene="mutH"
                     /locus_tag="ACHOTW_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP9842941.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            36276..36989
                     /gene="ygdQ"
                     /locus_tag="ACHOTW_000779"
     CDS             36276..36989
                     /gene="ygdQ"
                     /locus_tag="ACHOTW_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9842942.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            37127..37345
                     /gene="ygdR"
                     /locus_tag="ACHOTW_000780"
     CDS             37127..37345
                     /gene="ygdR"
                     /locus_tag="ACHOTW_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP9842943.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            37453..38493
                     /gene="tas"
                     /locus_tag="ACHOTW_000781"
     CDS             37453..38493
                     /gene="tas"
                     /locus_tag="ACHOTW_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP9842944.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(38525..39718)
                     /gene="lplT"
                     /locus_tag="ACHOTW_000782"
     CDS             complement(38525..39718)
                     /gene="lplT"
                     /locus_tag="ACHOTW_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP9842945.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(39711..41870)
                     /gene="aas"
                     /locus_tag="ACHOTW_000783"
     CDS             complement(39711..41870)
                     /gene="aas"
                     /locus_tag="ACHOTW_000783"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP9842946.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            42456..43487
                     /gene="galR"
                     /locus_tag="ACHOTW_000784"
     CDS             42456..43487
                     /gene="galR"
                     /locus_tag="ACHOTW_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP9842947.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(43494..44756)
                     /gene="lysA"
                     /locus_tag="ACHOTW_000785"
     CDS             complement(43494..44756)
                     /gene="lysA"
                     /locus_tag="ACHOTW_000785"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9842948.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            44878..45813
                     /gene="lysR"
                     /locus_tag="ACHOTW_000786"
     CDS             44878..45813
                     /gene="lysR"
                     /locus_tag="ACHOTW_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP9842949.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(45800..46492)
                     /gene="ygeA"
                     /locus_tag="ACHOTW_000787"
     CDS             complement(45800..46492)
                     /gene="ygeA"
                     /locus_tag="ACHOTW_000787"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP9842950.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(46621..48039)
                     /gene="araE"
                     /locus_tag="ACHOTW_000788"
     CDS             complement(46621..48039)
                     /gene="araE"
                     /locus_tag="ACHOTW_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP9842951.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(48354..49115)
                     /gene="kduD"
                     /locus_tag="ACHOTW_000789"
     CDS             complement(48354..49115)
                     /gene="kduD"
                     /locus_tag="ACHOTW_000789"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP9842952.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(49145..49981)
                     /gene="kduI"
                     /locus_tag="ACHOTW_000790"
     CDS             complement(49145..49981)
                     /gene="kduI"
                     /locus_tag="ACHOTW_000790"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9842953.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(50268..51449)
                     /gene="yqeF"
                     /locus_tag="ACHOTW_000791"
     CDS             complement(50268..51449)
                     /gene="yqeF"
                     /locus_tag="ACHOTW_000791"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP9842954.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            51704..52933
                     /gene="yqeG"
                     /locus_tag="ACHOTW_000792"
     CDS             51704..52933
                     /gene="yqeG"
                     /locus_tag="ACHOTW_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9842955.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            53393..54025
                     /gene="yqeH"
                     /locus_tag="ACHOTW_000793"
     CDS             53393..54025
                     /gene="yqeH"
                     /locus_tag="ACHOTW_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9842956.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWTEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            54359..55168
                     /gene="yqeI"
                     /locus_tag="ACHOTW_000794"
     CDS             54359..55168
                     /gene="yqeI"
                     /locus_tag="ACHOTW_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9842957.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRG"
     gene            55165..>55550
                     /gene="yqeJ"
                     /locus_tag="ACHOTW_000795"
     CDS             55165..>55550
                     /gene="yqeJ"
                     /locus_tag="ACHOTW_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFP9842958.1"
                     /translation="MIIKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIFN
                     DQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWVENYDTAHLTSK
                     KVNKFEIMDQVDDDRLAELIPDFYVFP"
BASE COUNT        13975 a        14752 c        13411 g        13412 t
ORIGIN      
        1 cccaagccag gaatgcccgt tttcggcata aacaattaat acggtcatct ggtttgtgtt
       61 ttttatgatt tattttctga aacgggcatg aaatttcgat tattaaagtg atggtagtca
      121 cataaagtca ccttccagct aataagtgtg accgccgtca tattacagag cgttttttat
      181 ttgaaaatga atccatgagt tcatttcaga caggcaaata ttcactgata tgaagcccga
      241 actcgctagt tttgcacttt tgaaaacata accgattacg tgcttaagct tctgaaccta
      301 agaggatgct atgggaaaca catcaataca aacgcagagt taccgtgcgg tagataaaga
      361 tgcagggcaa agcagaagtt acattattcc attcgcgctg ctgtgctcac tgttttttct
      421 ttgggcggta gccaataacc ttaacgacat tttattacct caattccagc aggcttttac
      481 actgacaaat ttccaggctg gcctgatcca atcggccttt tactttggtt atttcattat
      541 cccaattcct gctgggatat tgatgaaaaa actcagttat aaagcaggaa ttattaccgg
      601 attattttta tatgccttgg gtgctgcatt attctggccc gccgcagaaa taatgaacta
      661 caccttattt ttagttggcc tatttattat tgcagccgga ttaggttgtc tggaaactgc
      721 cgcaaaccct tttgttacgg tattagggcc ggaaagcagt ggtcacttcc gcttaaatct
      781 tgcgcaaaca tttaactcat ttggcgcaat tatcgcggtt gtctttgggc aaagtcttat
      841 tttgtctaac gtgccacatc aatcgcaaga cgttctcgat aaaatgtctc cagagcaatt
      901 gagtgcgtat aaacacagcc tggtattatc ggtacagaca ccttatatga tcatcgtggc
      961 tatcgtgtta ctggtcgccc tgctgatcat gctgacgaaa ttcccggcat tgcagagtga
     1021 taatcacagt gacgccaaac aaggatcgtt ctccgcatcg ctttctcgcc tggcgcgtat
     1081 tcgccactgg cgctgggcag tattagcgca attctgctac gttggcgcac aaacggcctg
     1141 ctggagctat ttgattcgct acgctgtaga agaaattcca ggcatgactg caggctttgc
     1201 cgctaactat ttaaccggaa ccatggtgtg cttctttatt ggtcgtttca ccggtacctg
     1261 gctcatcagt cgcttcgcac cacacaaagt cctggccgcc tacgcattaa tcgctatggc
     1321 actgtgcctg atctcagcct tcgctggcag tcatgtgggc ttaatagccc tgactttatg
     1381 cagcgccttt atgtcgattc agtacccaac aatcttctcg ctgggcatta agaatctcgg
     1441 ccaggacacc aaatacggtt cgtccttcat cgttatgacc atcattggcg gcggtattgt
     1501 cactccggtc atgggttttg tcagtgacgc ggcgggcaac atccccactg ctgaactgat
     1561 ccccgcactc tgcttcgcgg tcatctttat ctttgcccgt ttccgttctc aaacggcaac
     1621 taactgaaca tattttccga ataaagtgag gaatctgtaa tgaacaaaat cagcttaccg
     1681 aaaattggta tccgcccggt tattgacggt cgtcgcatgg gtgttcgtga gtcgcttgaa
     1741 gaacaaacaa tgaatatggc gaaagctacg gccgcactgc tgaccgagaa actgcgccat
     1801 gcctgcggag ctgccgtcga gtgtgtcatt tccgatacct gtatcgcggg tatggctgaa
     1861 gccgctgctt gcgaagaaaa attcagcagc cagaatgtag gcctcaccat tacggtaacg
     1921 ccttgctggt gctatggcag tgaaaccatc gacatggatc caacccgccc gaaagccatt
     1981 tggggcttta acggcactga acgtcccggc gctgtttacc tggcagcggc tctggcagct
     2041 cacagccaga aaggcatccc ggcattctcc atttacggtc atgacgttca ggatgccgat
     2101 gacacatcga ttcctgccga tgttgaagaa aaactgctgc gctttgcccg cgccggtttg
     2161 gccgtcgcca gcatgaaagg taaaagctat ctgtcgctgg gcggcgtttc gatgggtatc
     2221 gccggttcta ttgttgatca caacttcttt gaatcctggc tgggaatgaa agtccaggcg
     2281 gtggatatga ccgaactgcg tcgccgtatc gatcagaaga tttacgacga agccgaactg
     2341 gaaatggcac tggcctgggc tgataaaaac ttccgctatg gcgaagatga aaataacaaa
     2401 cagtatcaac gcaatgccga gcaaagccgc gcagttctgc gcgaaagttt actgatggcg
     2461 atgtgtatcc gcgacatgat gcaaggcaac agcaaactgg ccgatattgg tcgcgtggaa
     2521 gaatcacttg gctacaacgc catcgctgcg ggcttccagg ggcaacgtca ctggaccgat
     2581 caatatccca atggtgacac cgccgaagcg atcctcaaca gttcatttga ctggaatggc
     2641 gtgcgcgaac cctttgtcgt ggcgaccgaa aacgacagtc ttaacggcgt ggcaatgcta
     2701 atgggtcacc agctcaccgg caccgctcag gtatttgccg atgtgcgtac ctactggtca
     2761 ccagaagcaa ttgagcgtgt aacggggcat aaactggatg gactggcaga acacggcatc
     2821 atccatttga tcaactccgg ttctgctgcg ctggacggtt cctgtaaaca acgcgacagc
     2881 gaaggtaacc cgacgatgaa gccgcactgg gaaatctctc agcaagaggc tgacgcttgc
     2941 ctcgccgcta ccgaatggtg cccggcgatc cacgaatact tccgtggcgg cggttactct
     3001 tcccgcttcc ttaccgaagg cggcgtcccg ttcaccatga ctcgtgtcaa catcatcaaa
     3061 ggcctgggac cggtactgca aatcgcggaa ggctggagcg tggaattgcc gaaggatgtg
     3121 catgacatcc tcaacaaacg caccaactca acctggccaa ccacctggtt tgcaccgcgc
     3181 ctcaccggta aagggccgtt tacggatgtg tactcggtaa tggcgaactg gggcgctaac
     3241 catggggttc tgaccatcgg ccacgttggc gcagacttta tcactctcgc ctccatgctg
     3301 cgtatcccgg tatgtatgca caacgttgaa gagaccaaag tgtatcgtcc ttctgcctgg
     3361 gctgcgcacg gcatggatat tgaaggccag gattaccgcg cttgccagaa ctacggtccg
     3421 ttgtacaagc gttaatacat tactccctga tgtgatgccc ggtcgctccg gctgccgggc
     3481 ctgaacaagc aagagtggtt agccggataa gcaatgttat ccggctatat tgcaggagcg
     3541 atcatgaaac aagaagtgat cctggtactc gactgtggcg cgaccaatgt cagggccatc
     3601 gcggttaatc ggcagggaaa aattgttgcc cgcgcctcaa cgcctaatgc cagcgatatc
     3661 gcgatggaaa acaacacctg gcaccagtgg tctttagacg ccattttgca acgctttgct
     3721 gattgctgtc ggcaaatcaa tagtgaactg actgaatgcc acatccgcgg tatcgccgtc
     3781 accacctttg gtgtggatgg cgctctggta gataagcaag gcaatctgct ctatccgatc
     3841 attagctgga aatgtccgcg aacagcagcg gttatggaca atattgaacg gttaatctcc
     3901 gcacagcggt tgcaggctat ttctggcgtc ggagccttta gtttcaatac gttatataag
     3961 ttggtgtggt tgaaagaaaa tcatccacaa ctgctggaac gcgcgcacgc ctggctcttt
     4021 atttcgtcgc tgattaacca ccgtttaacc ggcgaattca ctactgatat cacgatggcc
     4081 ggaaccagcc agatgctgga tatccagcaa cgcgatttca gtccgcaaat tttacaagcc
     4141 accggtattc cacgccgact cttccctcgt ctggtggaag cgggtgaaca gattggtacg
     4201 ctacagaaca gcgccgcagc aatgctcggc ttacccgttg gcataccggt gatttccgca
     4261 ggtcacgata cccagttcgc cctttttggc gctggtgctg aacaaaatga acccgtgctc
     4321 tcttccggta catgggaaat tttaatggtt cgcagcgccc aggttgatac ttcgctgtta
     4381 agtcagtacg ccggttccac ctgcgaactg gatagccagg cagggttgta taacccaggt
     4441 atgcaatggc tggcatccgg tgtgctggaa tgggtgagaa aactgttctg gacggctgaa
     4501 acaccctggc aaatgttgat tgaagaggct cgtctgatcg cgcctggcgc ggatggcgtg
     4561 aaaatgcagt gtgatttatt gtcgtgtcag aacgctggct ggcaaggagt gacgcttaat
     4621 accacgcggg ggcatttcta tcgcgcggcg ctggaagggt taactgcgca attacagcgc
     4681 aatctacaga tgctggaaaa aatcgggcac tttaaggcct ctgaattatt gttagtcggt
     4741 ggaggaagtc gcaacacatt gtggaatcag attaaagcca atatgcttga tattccgtta
     4801 aaagttctcg acgacgccga aacgaccgtc gcaggagctg cgctgttcgg ttggtatggc
     4861 gtaggggaat ttaacagccc ggaagaagcc cgtgcacaga ttcattatca gttccgttat
     4921 ttctacccgc aaactgaacc tgaatttata gaggaagtgt gaaatgctga aaacaatttc
     4981 gccgttaatt tctcccgaac tattgaaagt gctggcagag atgggacatg gagatgaaat
     5041 tattttttcc gatgctcact ttcccgccca ttcgatggga ccgcaggtga tccgcgctga
     5101 tggcctgttg gtgagcgact tgctccaggc gattatcccg ttatttgagc tggacagtta
     5161 tgcaccgccg ctggtgatga tggcagcggt agaaggtgac actctcgatc ctgaagtaga
     5221 acgacgttac cgtaatgcgc tttcactaca agccccgtgt cctgacatca tccgcatcaa
     5281 tcgttttgcg ttttatgaac gggcgcaaaa agcctttgcg atcgttatca caggcgaacg
     5341 agcgaagtac gggaatattc ttttaaaaaa aggggtaaca ccgtaatctc ataccggtac
     5401 gcccgcatga cgcgggcggt tatcgaatga tggggtgaaa aatatgaaag cggcacgcca
     5461 gcaagcgata gtcgacctgc tgctgaacca taccagcctg accacggaag ctctctctga
     5521 acagctaaag gtcagtaaag aaaccattcg tcgcgatctc aatgaattac agacgcaggg
     5581 taaaattctg cgcaatcatg gacgcgctaa atatatccac cgtcaaaatc aagacagtgg
     5641 cgatcccttt cacatcaggc tgaaaagcca ttatgcgcat aaagcagata tcgcgcgcga
     5701 ggcgctcgcg tggattgaag aagggatggt gatagcctta gacgccagtt caacttgctg
     5761 gtatctggca cgccagttgc ctgacatcaa cattcaggtc ttcaccaata gccatccgat
     5821 ttgccatgaa ctcggtaaac gcgaacgcat tcaactgatc agttccggcg gcacacttga
     5881 gcgcaaatat ggctgttacg tcaatccctc gctgatttcc caacttaaat cgctggaaat
     5941 cgatctgttt attttttctt gtgaagggat cgatagcagc ggcgcactgt gggactccaa
     6001 tgctatcaac gctgattaca aatcaatgct attaaaacgc gccgcgcaat cgttgttatt
     6061 gattgataaa agtaaattta atcgttcagg ggaagcccgc atcgggcatc tggatgaggt
     6121 aacgcacatt atttctgatg aacgccaggt tgcaacttct ttggtaacag gctgacggaa
     6181 agggtagcag gccggagacg acgccccggc cttgcctgtt atcgctcgtc gcgacgacca
     6241 cccaccgcag cccagatacg gcggacgtgc accgtcactt cttcgcgatc gtgatacaac
     6301 tgccgcgcct gaatctgagc atttatgcca tgttcatcaa gctgtgcctg aatatacgcc
     6361 agattgtgtg acacttcttc gtagcgtttt ttcatcggca gtttgaggtt gaaaatggtt
     6421 tcacgacacc agccattaac cagccactgc gccatcaatg ccgcaacttt cgccggtttt
     6481 tcaaccatat cgcataccat ccaggagata ttgctgcgcg tcggacggaa tttgaaaccg
     6541 tcttcccgca gccacgtcac ctgtccggta tccatcagac tttgcgccat cgggccgttg
     6601 tcgacggaat aaacccacat gttgcgcttc accagttggt aggtccagcc gccagggcaa
     6661 gcgcctaaat ccaccgccca catcccgttc gccaggcgtt catcccactc atccgcagga
     6721 ataaacacat gaaatgcctc ttccagtttg agcgtggaac gactcggcgc atctgccgga
     6781 aatttcaggc gcggaatgcc catatagaac ggcgaattat tgttgctgta tgagtaaccg
     6841 gtatagcagc agcctggtgc aataaagaat acatgcacaa ccggacgctt cggcgtttca
     6901 tagttcgcca gcaccccggc atcgcgcaag gcagcgcgta gcggcacggt gaatttacgg
     6961 cagaatttca gcaactcttt gctttcgttg gtatcggcaa cttcaacacg cagttcaccg
     7021 cccttctcta ctacgccctg caacatgccg acaatggggg taatacgatc ttctggcggc
     7081 aaatgctgca agagttcccc caccacaaac cactggcggg caaaaattaa tgaactgaac
     7141 ggcagctcac ggattaactt atcgccatca tcaggttgat aacattcata aatgacataa
     7201 cccgcattct ctttcacgcg ggcaaaaccg aaaatctccc gctggccggc tttatcggta
     7261 atttctgcgg cgcactcttt ttcaaagccc ggacggcaca gcaatacaac cttattcatg
     7321 acttacgccc ttgcgcttta aacggatagc accaactaac attaacgccc agcccgcaag
     7381 gaagctcacg ccgccaaccg gagtgacaaa cgcccacaaa cgcagatggg acagtgccag
     7441 gcaataaagg ctgccgctga acagcaccgt gcctaacgcg aggaaaacgc tactccagta
     7501 aaaccagata ctgatgcgac gctgcattgc caccgccaga cctaagatcg ccagcgtatg
     7561 aaacgcctgg tattcgaggc cggtctggat ccagcccatc tcaacggccc ccatggtttt
     7621 acttaacaca tgcgcgccaa aagcgcccag tgccacaaaa atgaagccgc taatggcggc
     7681 gaaaatcagc ataaaacggc tggtcatggt cgtaccctac gtaaattatt gttcataacg
     7741 aaagcggaat ttttcttgtt cagcagcggc tttcgccagg atccattggc gaaaggcggc
     7801 tattttaccc agttctgcct ggctgtcatg acaaaccaga taaaaagcgt ttttactgac
     7861 cagaacatca ttaaacgggc aaacaagacg tccggcttca atttcagatt gcgccatcac
     7921 gttatttgcc agcgccactc cctgcccgtg aatagccgct tgcagcacca tggcgctatg
     7981 gctaaaaatt ggcccttgct gaacgttgat atgatttaac cccaattgac gagtatatgt
     8041 ctgccagtca cggcgtgacg catcatgtaa taacgtatgt ttagccagat cttccggggt
     8101 cttcaagggt ttttcgccag tcagcagtag cggcgaacac accggcaata aatattcggc
     8161 gtacagtttt tccacccgta gccccggcca gttgccccga ccataaaata tcgccacatc
     8221 aacatcatcc gccagcttat cttcctgacg atcaaccgcc tggattcgaa cgtcaattcc
     8281 cggataagct gaattaaagc tggaaagtcg cggaaccaac caatgaatgg cgaaactggg
     8341 gagtaaactg accgtcaacg cccccttggc gctacgggcc tggagtttac gcgtcgcttc
     8401 ggttaattgc gaaaatatct ctttgatatc gaggaaatag ctttgccctt cctcggtcag
     8461 caggagtgaa cgattacggc ggcggaacag ttttagcccc aaaaaatcct caagagactt
     8521 gatttgatga cttactgcgg cctgggtcac aaaaagctct tctgctgcgc gagtgaaact
     8581 taaatggcgt gctgcggcat caaaaactcg taaggcattt agcggtggta atcgtttaga
     8641 catggctttt aaactttgat gttaaatgaa tttaacaatt agatcacact gtgtaaccta
     8701 ttagtttttt taatctgagc cattataaat tgtccgttga gcttctacca gcaaatacct
     8761 atagtggcgg cacttcctga gccggaacga aaagttttat cggaatgcgt gttctggtga
     8821 acttttggct tacggttgtg atgttgtgtt gttgtgtttg caattggtct gcgattcaga
     8881 ccatggtagc aaagctacct tttttcactt cctgtacatt taccctgtct gtccatagtg
     8941 attaatgtag caccgcctaa ttgcggtgct tttttttacc ttgcgatcgc gaattactga
     9001 tccagttcga ccatctcttt cacgtcggta cggttgatct gctgtttgtt gccattagcg
     9061 tctttatacg aaatcatacc ggtatcgtta tcagtctgtg gtttgccgtc agagacgatg
     9121 gtacgtccgt cattggtgtg catcacatag ttcgaaccgg aacaggcgct cagggcaaaa
     9181 gtcagcatac aggcagaaat aattgcggca gtctttttca tcatcttctc cttaaagcga
     9241 ttaataaatc ttaatagtat tgattaacag gctaaaatta acgcctaaca ctattcagca
     9301 tatgttactg acgcggcttc gccaggatat ccagataatt ctgatggtta gcactctcct
     9361 tgtatcaaag tgaattttgc gtcacgatcg gtgcatcaag ccgaggagta ccatgaacgt
     9421 ttttaatccc gcgcagtttc gcgcccagtt tcccgctcta caggatgcgg gcgtctatct
     9481 cgacagcgcc gcgaccgcgc ttaaacctga agccgtggtt gaagccaccc gacagtttta
     9541 cagtctgagc gccggaaacg tccatcgcag ccagtttgcc gaagcccaac gcctgaccgc
     9601 gcgttatgaa gctgcacgag agaaagtggc gcaattactg aatgcaccgg atgataaaac
     9661 tatcgtctgg acgcgcggca ccactgaatc catcaacatg gtggcacaat gctatgcgcg
     9721 tccgcgtctg caaccgggcg atgagattat tgtcagcgtg gcagaacacc acgccaacct
     9781 cgtcccctgg ctgatggtcg cccaacaaac tggagccaaa gtggtcaaat tgccgcttaa
     9841 tgcgcagcga ctgccagatg tcgatttgct gccggaactg attactcccc gtagtcggat
     9901 tctggcgttg ggtcagatgt cgaacgtaac tggcggttgc ccggatctgg cgcgagcgat
     9961 tacctttgct cattcagccg ggatagtggt gatagttgat ggtgctcagg gggcggtgca
    10021 tttccccgcg gatgttcagc aactggatat cgatttctat gctttctctg gtcacaaact
    10081 gtatggcccg acgggtatcg gcgtgctgta tggcaaatca gaactgctgg aagcgatgtc
    10141 gccctggctg ggcggcggca aaatggttca cgaagtgagt tttgacggct tcacgactca
    10201 atctgcgccg tggaaactgg aagcaggaac gccaaatgtg gctggtgtca taggattaag
    10261 cgcggcgctg gaatggctgg cagattacga tatcaaccag gccgaaaact ggagccgtag
    10321 cttagcaacg ctggcggaag atgcgctggc gaaacgtcca ggctttcgtt cattccgctg
    10381 ccaggattcc agcctgctgg cctttgattt tgctggcgtt catcacagcg atatggtgac
    10441 gctgctggcg gagtacggta ttgccttgcg ggccgggcaa cattgcgctc agccgctact
    10501 ggcagaatta ggcgtgaccg gcacactgcg cgcctctttt gcgccatata atacaaagag
    10561 tgatgtggat gcgctggtga atgccgttga ccgcgcgctg gaattattgg tggattaatg
    10621 acgaacccgc aattcgccgg acatccgttc ggcacaaccg taaccgcaga aacgttacgc
    10681 aataccttcg caccgttgac gcaatgggaa gataaatatc gccagttgat catgctgggg
    10741 aaacagcttc cggctttgcc agacgagtta aaagcgcagg ctaaagagat tgccggatgc
    10801 gaaaaccgcg tctggctggg atataccgtg gctgaaaacg gtaaaatgca cttctttggc
    10861 gacagcgaag gacgtatagt gcgcggcctc ctggcggtgc tgttgactgc cgttgagggg
    10921 aaaaccgccg ccgaattgca ggcacagtcg ccgctggcat tgtttgatga gctgggattg
    10981 cgtgcgcaac ttagcgcctc acgcagccag gggttaaatg cgttaagcga ggcgattatc
    11041 gctgcgacga agcaggttta agcacagaac cgggtcggat aagacgtttg cgtcgcatcc
    11101 gacctgattg tttacccctg acgcgccgct ttcgccatca tcttcttcag cgcatgagaa
    11161 accgcaacaa aaccaaaggt ggcggtcacc atcgttgccg cgccaaatcc agatgcacaa
    11221 tccatccgct ttggtccttc cgccgtggct ttcatcgcac acaccgtacc gtctgactgc
    11281 ggatacacca atgcttcggt agagaacacg caatccacac cgagcttacc tttactgttt
    11341 ttcactacgc caaaatcgct tttcaggcgc tcacgcaact tcgccgccag cgggtcctga
    11401 atcgttttcg ccagatcggt aacctgaatc tgcgttgggt caatctgccc acccgcgcca
    11461 ccggtcgtga ccagcgggat tttattgcgc cgacaataag caatcagcgc cgctttgggc
    11521 cgtacactat caatggcatc aatcacgtac gaataaccca cgctcatatg ctgcgctacg
    11581 ttatccggcg tcacgaaatc atccaccacc gttacacagc actccgagtt aatctggcga
    11641 atacgttccg ccatgacttc cgcttttgcc agcccaacgt tatcgcgcag ggcatgaatt
    11701 tgccgattgg tattggtgac gcacacatca tccatatcga taagcgtgat tgcgccaatc
    11761 cccgtgcgcg ccagcgcttc cgccgcccag gaaccgacac cgccaatacc gaccacacaa
    11821 atatgcgcgt cagcaaacag ttgcaacgct ttttcaccat acagacgcgc tgtgccacca
    11881 aaacgctgac gccatgcatc actaattacc acagacatag cacctcagaa ttaaaaaggg
    11941 cgaggttatc cctcacccta aacgcataaa tgcctgatgc gctacgctta tcaggcctac
    12001 ataactcctg caatattttg gatttgcatg atttgtaggc cggataaggc gttcacgccg
    12061 catccggcat gaacaacgcg cacttagtca ataatcagga tgtgaggtta cccctcaccc
    12121 tgtcatatcc gtaaaaacgg catacagaat atcacatcag ccgctaaaga cgttacctgc
    12181 gcccggggcg gttttcagca cccagacacg tccatagtgg ttgtaccaac ctgcgcggtg
    12241 tccggcttcc ggcccgatcc cttgataaat atcgaagtgt tggcctttga ttgcgccacc
    12301 gacatccaac gccaccatca gacgcaattc gtactgaccg ttaaatttgc cgttattatc
    12361 cagcagcggc acttctgcca gcaaggttgt acctggtggg ataatggaac gatcagaggc
    12421 aacagaagca cggccaacca gcggcaccgc acttgcccct ttcaccggtg caaaagattg
    12481 cggtttaaag aaaacgaaag acgggttctg ttccagcagc tcgcgaacct cggcttcact
    12541 gtgtgtttcg ccccaatgac gaatcgcctg catcgacata tcttcttttt tcacttcgcc
    12601 acggtcgatc agcaccttac caatgctgcg ataggcatga ccgtttttcc ctgcatagct
    12661 gaaaaagtta agcggactgc catcaccaaa gtcgatatac ccactaccct gcacatccat
    12721 aatgaaatta tccatcagag agttgctgta agcgaggatg tacttatcgc tcaatgcacc
    12781 cgcgtagatc tccgcacgag acggcagacg accacgtttt ggcggcatac ggtaaatagg
    12841 atactggaac tcgccctggc gggtatggcg cgcctgaatt accggcgtgt aatagcctgt
    12901 aaactgcacg ttaccatagt tgtcggcacc ttccatctgc caggcatcaa tgccgaactg
    12961 gcgcatattg cgggtatcac cgcctgcgcg cagccactct tgcaccgcgt tataaacatt
    13021 actctggttg ccatacagac gcggtgacga attacggata tggttaattt gctcggcaaa
    13081 atcaccggcg ttaatcggcg cgccaacggc atctggctgg ttcaccagag agaaaggctg
    13141 ggtaaatttc ccgtctttat attgctgtcc gcgatcggtt ggtttggaag agcaggcggc
    13201 aagcattgcc acaaccgtgc ccataagaag gtactttacc caacgtcctt tcattgttct
    13261 cttcttcagg ttaaaaaata aggcgcaacg aagataacaa accaccgcgt ttaatgaaat
    13321 gagcagcctc tccctgacgc aaattttgca caaaaaatag gctttagtga tttgtttttg
    13381 ttcaaaatca tgccagatcc gtgatcgggg taaaaaaaag gttgcatgaa aacgcgagcg
    13441 gagtatagtg cgcatccacg gacgcggggt ggagcagcct ggtagctcgt cgggctcata
    13501 acccgaaggt cgtcggttca aatccggccc ccgcaaccaa ttattgaaca ccctaacggg
    13561 tgtttttttg tttctggtct cctataaaaa agcgccattc agcgcctttt tatcatcccc
    13621 ttctcgccag ggtcgcccca tcggcaaaat acgctttaat tcccgcaaga atagactccg
    13681 caacttcctg ctggaaggtc gccgttttca gtttacgctc ttcctcaacg ttactgataa
    13741 acgccgtttc gaccagaatg gagggaatat ctggtgcctt tagtacggca aacccggcct
    13801 gttcaacttg atttttatgc agcttgttga ttttaccgag cttattcagc accgctttac
    13861 caaacttaag gctgtcagca atggtcagcg actgcaccat atcgaacatg gtgtggtcga
    13921 catagcggtc accgcttttg ctcacgccac caatcaagtc ggaggcgttc tgggtttgtg
    13981 ccagatattt tgccgcagta ctggttgcac ctttggttga gagcgcaaac accgaggaac
    14041 cgctcggctg gcgactggta aaggcgtcgg catggataga gacaaacagg tcagcacgct
    14101 gtttctgagc ttttgctacg cgcacttgca acggaatgaa gatgtcttca ttgcgcgtca
    14161 tgtacacctt catattgccc tctttctcga tcagagagcg caaacggcga gctatttgca
    14221 ataccacatc tttttcgcgc gttttgtatt tccccaccgc accggagtct tcgccaccgt
    14281 ggccagggtc aagcataatg acaatcggac gatcgcgccc tgctttacct ggttgtggac
    14341 cactttgcgc tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc tccagcagcg
    14401 ccagcagcgg gtcctgcata tcctgtgcat tggccggata gaggtccatc accagacgct
    14461 ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt tgctttaatt
    14521 caaaaaccat ccgtacggtt tgcgggtcaa attgcccgac gcgcgccgac ttgatgaacg
    14581 ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac cgagttcagg tttacatctt
    14641 cgatatccac caccacgcgt tccgggttac tcaacgcgaa ctgcttatat ttcagctgac
    14701 gatttgattc taccgtcacg cgggtgtagc tggacgcagg ccagacgcgc accgccacga
    14761 cctggctgac cgcagccaga ctgacctgac ttacgctcaa tagccacatg gcacccgcgc
    14821 cttgcagtaa acgacgacgg ctgattgcag tgttggatcc tgacatgcct ctcccgagca
    14881 aaagaaatct aagctgtgta acaagtaaac gactaatttg accggaaact ttagcgaaag
    14941 acgcataatc tgtcatctaa taaacggtaa acattctttt tatattcacg ccattactga
    15001 taaaaaagtc gctctcgcat aaaatttaca cttgcaccct gcgaaaaaac agaataaaaa
    15061 tacaataaat tcgaataata atgcaaagag gtgtgccgtg gtaaaggaac gtaaaaccga
    15121 gctggtcgag ggattccgcc attcggttcc ctatatcaat acccaccggg gaaaaacgtt
    15181 tgtcatcatg ctcggcggtg aagccattga gcatgagaat ttctccagta tcgttaatga
    15241 tatcgggttg ttgcacagcc tcggcatccg tctggtggtg gtctatggcg cacgtccgca
    15301 gatcgacgca aatctggctg cgcatcacca cgaaccgctg tatcacaaga atatacgtgt
    15361 gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg ggaacattgc aactggatat
    15421 tactgctcgc ctgtcgatga gtctcaataa cacgccgcta cagggcgcgc atatcaacgt
    15481 cgtcagtggc aattttatta ttgcccagcc gctgggcgtc gatgacggcg tggattactg
    15541 ccatagcggg cgtatccggc ggattgatga agacgcgatc catcgtcaac tggacagcgg
    15601 tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact ggcgagagct ttaatctgac
    15661 ctcggaagag attgccactc aactggccat caaactgaaa gctgagaaga tgattggttt
    15721 ttgctcttcc cagggcgtca ctaatgacga cggtgatatt gtctccgaac ttttccctaa
    15781 cgaagcgcaa gcgcgggtag aagcccagga agagaaaggc gattacaact ccggtacggt
    15841 gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc gtgcgtcgct gtcatttaat
    15901 cagttatcag gaagatggcg cgctgttgca agagttgttc tcacgcgacg gtatcggtac
    15961 gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca acaatcaacg atatcggcgg
    16021 tattctggag ttgattcgcc cgctggagca gcaaggtatt ctggtacgcc gttctcgcga
    16081 gcagctggag atggaaatcg acaaattcac cattattcag cgcgataaca cgactattgc
    16141 ctgcgccgcg ctctatccgt tcccggaaga gaagattggg gaaatggcct gtgtggcagt
    16201 tcacccggat taccgcagct catcacgggg cgaggttctg ctggaacgca ttgccgctca
    16261 ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc acgcgcagta ttcactggtt
    16321 ccaggaacgt ggatttaccc cggtggatat tgatttattg cccgaaagca aaaagcagtt
    16381 gtacaactac cagcgtaaat ccaaagtgtt gatggcggat ttagggtaag cgatgaaaat
    16441 cgtcggatgc gacatgcgta acactcgtac gtcgcatccg gcaattacgt ttattcccgt
    16501 gaactaaaca acgccgccag accactgcgc cgctcagtac gagtggcgat tgccgcactt
    16561 aatatgcgct catcggcata cagcgacaga cggcgacgcg cgcgggtcac cgcggtataa
    16621 accagctctc gcgttactac cggcgtgcgt tggctcggta aaatcaacgc cgcatggtcg
    16681 aactccgatc cctgcgattt atgtaccgtc atcgcccacg tagtttcgtg ctctggcaag
    16741 cgactcggtt gcacagactt aatattgccg tccggcatcg caaaccagac gcgcgtcccc
    16801 tgcccgcgat ccagcgcaat accgatatcg ccattaaaca acccaagcgc gctgtcatta
    16861 cgggcaatca tcaccggtcg gccttcgtac caacgagagt gcggattacg atgaattttg
    16921 cgcttctgtt gcataaactg ctcaattcgc tcattcagtc cagccacgcc aaatggccct
    16981 tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct gaatgattaa atccggctcg
    17041 gcacgcgctt gcagcagatc cagataacgt ccataacccg caagagcttc ctcaagcatc
    17101 gcaatataat cttcgccgct ctgtaaaagc cgtttttcaa tatcagtaaa atcctgctga
    17161 aaaacggttt tcactgccgt tttatcacca cggttgatcg ccgcagctaa ctgaccaatg
    17221 ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc agagactgtc gcgcaaagat
    17281 gccgcttctg tgccagttcc tgccggaacg tgagttcccg tcaggcggct tagctgcccg
    17341 gcacgctcgg cggtaaagcc ggcattggca taagcgcaga tatcgcccag cacagcccca
    17401 gcctcaaccg aggccagttg atcacgatcg ccgagaaaga tcactcgcgc atgatcgggc
    17461 aaggcgtcga tcagtctcga catcataggc agatcgatca ttgacgcttc atctaccacc
    17521 agcacatcaa gatgcagcgg gttaccggca tgatgacgta aacgctggct acccggctgc
    17581 gcacccagca atcggtgcaa agtactggca tcttccggaa tacgtttctt ttgttcatcg
    17641 gtcagcggta actgtcgcaa agccttgccg agagattcgg ttaagcgcgc ggcagcttta
    17701 cccgttggtg cagccagacg gatacggcag cgttcgccgt cggccatttg aattaacgct
    17761 gccagcaact tcgctacggt ggtcgtttta ccggtgccag ggccgccgga aatcaccgag
    17821 atccgccgcg tcagcgccac tgctgccgcc actttttgcc agttaatttc atcaccagtc
    17881 ggaaagagtt tatccagggt ttgcgccaac aaggcttcat ccacctcgat ggcatgattc
    17941 acctcattga agaagcgcgc taccgtccgc tcgttacacc acatgcgatt caaataaaga
    18001 cgatcgccac agaggatcat cggcgtgggt tcatctcccc ggctgaccgc ttgtgaagcc
    18061 agcaagcatt cttcccaatt ttgtagctca ccgatttcac tgacacaggt cgccaacagc
    18121 ggatgcgact cctcattatt ttccagtcgt gaaagcggca aacaaacgtg cccctctccg
    18181 gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag gatgttcatc tcccgccacg
    18241 gtcagggcaa actgcacgtc cagcgggcgt agctgtttgt gctccacagc ttccagtaat
    18301 tgcttttgca atttcattac gcctcctcca gagtcatacc ggcaaacatc tcatccatca
    18361 ggtcaatcaa cccggcgttg gggcgggttg tgtaaatccc ttgttgcgga tgttctttat
    18421 caacaccacg caggaacaga taaataacgc cgccaaagtg gcgctcatag tcgtaatcag
    18481 caatgcgatg gcgcagataa cggtgcagcg ccagggtata aagctgatat tgcagatcat
    18541 agcggtgtgc ctgcattgcc gctgccatag cctgttgggt gtaagccgaa ctatcttcac
    18601 ccaaccagtt ggatttatag tcgagcaggt aataacgccc ttcgtggcgg aacaccaggt
    18661 cgataaagcc ttttaacatg ccacgtgcct gcatgaactc cagcggcggg cagccagcgg
    18721 atagcgggtc aaactggcga attagtgcat caagctggct ggcgatgagc ggttcgctaa
    18781 tcggcaggta aaactccatc tccacctgtt tatcgcggtc ggaaagctga ctcaggctaa
    18841 cacccgtttc attgagaggt gcctggagga cagccgtgat ccactcggtc aacaccggtt
    18901 cccactgcga ttcaaagccg ccgagttcca gtttttcctg tacccagttc gggtcaaccg
    18961 gctgggtaaa attgaggtct tcaaacaaac tgtgcaagaa tgtccccggt gacgcaccgc
    19021 gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac gctgaccacg cctgcggcat
    19081 caacatccag ccgtggcatc agatcctgag cgataccgtg accacgctgc tgcaagccgg
    19141 agtagctggt gacacgccag ttatcgccgg gcaatcgttg tagcgtcctc gcattcagtt
    19201 ctgcagttaa agcatcatta acctgccagg gctggttatc accagtttgt gccgtttgcc
    19261 aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc gcatcttgcg
    19321 gttccccttt ttgcagcaaa cgcccgaggg cactttggtg gacgtcggtg tcaccttttt
    19381 tatcgccacg acggcgcacc agcggtgcaa cgccgagact gcaatgccaa accgaacgcg
    19441 tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg ttcgacctcc gcaagatcga
    19501 cgctttctgg cgcagcgtta agatccagaa cagcctcaaa cgagtggcga tcgtgataaa
    19561 acgcctgatc ctggacgcgg aaattggtaa taaatggcaa ccagaccagc ggatattcca
    19621 gcccttttga tttatggatc gtgacaatct gtaccagatg tttatcactt tcgaggcgca
    19681 tttgttggct ggaggcatta ctgtctggct cgaggatatg ttgcgataac cagcgtacca
    19741 gcgcatgttc actttccagc tgcgttccgg cttcttgtag cagttcgctg atatgcaaga
    19801 tatcggtaag acgccgctca ccgcctgccg ttgccagcaa gttttctgca atgttacgcg
    19861 ccgacatcag cgcccgcatc atcggcataa cgccacgttt gcgccagatt tgccgataac
    19921 catcgaactc ttcgaccacc gcgtcccacg catgttcgtc attattcagc gtttcgatat
    19981 ccagcgcgtt cagccccatc attgacgttg ccagcgcact acgcagggtg ttctcacgtt
    20041 cgggcgtcat caccgcctgc aacaaccaaa gcatttcctg cgcttccaga gtttcaaaaa
    20101 cactgtcgcg gttcgaaagg taaacggaag ggatttccag caacgttaag gcatcgcgca
    20161 cctgggcggc ctcctggcgg ctgcgcacca gcacactgat gtccgaagca cgcaccggac
    20221 gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg tccggcttgt agccagtcgc
    20281 gaatttgcgc agcacttacc tgcgccatgg tactttgata gtcgccaacg ccgcaacttt
    20341 cgccttccat cagccacatt ttcatcgcag gctgcgtttc acctttaaag acaaaacgta
    20401 acgcctgatt tttcccggct gatttcactg gaataaacgg tatttcgcga aacatgaacg
    20461 cgtcatcagt ctggctgaaa agcttgttca cactgttcac cattccaggt gcggagcgcc
    20521 agttggtgtc taaagtgtag tgggcgtgaa cttcactacg cgccttcata taagtgaaga
    20581 tatccgcacc ccggaatgca tatatggcct gcttcgggtc gccaatcagc aacaatgcgg
    20641 tttccggctg atggtgccag atacggcgaa aaattcggta ctgctggggg tcggtatcct
    20701 gaaattcatc gatcattgcc accgggaatc gcgtacggat cgccgctgcc aacacctcac
    20761 cgctttcgct acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa cccaattcgc
    20821 cacggcggcg tttttcacgc gctactgttt cgcggatctc agccaatgcg cgggtgatca
    20881 ccagatcgcg gatcgacaat ggttctgcaa gcagttgatc gatcgcctca aacagtggat
    20941 gtcgcggggt ttcccccccg gccttcgtgc gatcttctaa gaaacgctgg gagaattttt
    21001 ccagcgactc cggcaactga taactgtttc tctcttcttc tgcccaggcg ctgatcttgt
    21061 cgatccattt agcctgattg ctacggttaa acttgcgtcg atcaatacca gaagattcga
    21121 tcagcgcatc cagttcaccc actgcgtcgc gccactgctg ttttaccgta tcaatacgcg
    21181 ccacaatttg cgcgtgacgg gaagccagcg tttcatcatc gggcggcggt gctttgataa
    21241 ccggcgcttc accttgcaga taacgattaa tatcgcgcag caacgcctgc ggccctttcc
    21301 aggtttcaaa gacgacctgg gctatttcac gcggcagcgg gtagcagtgg cgacgccaga
    21361 aatcggcgca ggcctggtaa cgtagcagag actcatcttc aatcagctgc tgctcaaaca
    21421 gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg gcaaaaaccg tgaatagtaa
    21481 agactgccgc ttcatccatc tgccgttcgg ctaacaacaa ccacttcgcg gcttgcgctt
    21541 tatcgtcgat ctcttccagc agacgttcgt acagtggatt gtcggtggtt tcacgcagac
    21601 aggcgatgcg caactcatgg atattgctac ggatacgacc acgcaattct gccgtggcag
    21661 cctcggtaaa ggtgaccacc agcagttctt caacggtcag cgggcgggga aaggcggcgg
    21721 aaccgcctag tccaagtaac aggcgcaaat agagcgccgc aatcgtaaag gttttgcctg
    21781 tgccggcaga ggcttcaatc aggcgctcgc cctgaaaggg caagcgcaaa ggatctagtg
    21841 tctcggcgac atcactcatt cttttcactc atcaggggca ttgtttgctg caacgcgctg
    21901 acgttctccc acactttcca gccttcagga tgcacatatt cggctttccc gttctggctg
    21961 ccggaaatct gcgacagaat agccatgcct tgcggctcga ccaccgcctg atggaagaaa
    22021 tcagcaagtt tttgcggcgt cagcagtttt atctgggcca cgattttatc acgcgaatcg
    22081 aagcgcatat tgccgcgatc gaaatcttta cttaacttca atgcttcttc gccgagcgtt
    22141 tgcggtgcct gcagcatctg ggtaattacc gcctgctgga tttgcgcaaa ctcatctggc
    22201 ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg ccttgtaacg ctcccacaag
    22261 aatgaaggct gtttatcatt gctttgcaaa aggaagccca tgccccactg acgccccacg
    22321 ctcattggaa acgcaaacac ggcatagccc aattgttctt cggtacgcaa ctgattgtag
    22381 aaccacggct gtacgatctg ccccaacaga gagctatagg ctgagcttgt gtattcatcg
    22441 tagccagtcg gtacaaatac cgctgccagt gcggagtcgg tgctgttacc ggctttttca
    22501 aagatgacgg attgtttttt atcgaccact acatctttgt ttcgacacca ctctgaacca
    22561 tcagcgccca actgtttttg cacatcgcgt gccagcgttg ttgcctgggc ctcggtcatg
    22621 ttgccgataa ccataaactc tggtcgagcc cctgatttta aggcgtcacg ataagccagc
    22681 acctctttca acgtaatgga gggcaaaatt ttacgccgtt catctcgcga gaagtacggc
    22741 acttgcgaga gcatctgcgc gggcataatc gcctgctcaa acgctttacc cttttctgcg
    22801 gaatccatca tctggttata ccaggacttc gcctgctcaa gctgatcttc cgtagcggta
    22861 tagctaaagt atccctcgag caatgcctgg aacagctgcg gcagacgctg ggtgtaacca
    22921 ttagcattaa ccataaggcc gttgttagcg ttggtggaaa aacttatgcc accaaccgac
    22981 gcctggttgc ttaactgatc aagcgccagc cctgcgagat aatcattgag cgcaaacatc
    23041 acctgattgc gggcgctgtc catggctttc ggattacgca aaatcaggct gacatcagct
    23101 ttgggttcgc tggaaaaata acggcttggc gcatacacca cgcgcagatt cgactcatca
    23161 acaatcagct ctggatggtc atatttcttc tctgacttaa tcagcgagaa atcgtcagga
    23221 atataggggt taagctccgg taaggagagc gcaatattgg cagctttttg ctgccagtca
    23281 gcgaaagttt gttcgctgat tttatcgacc tgatacggcg catcgacaaa ataagccgtt
    23341 ttattgtgcg gctctttcgg gctgatatac cagatacgcg cattctgcgg cgtcatcatc
    23401 gccagacgtt cttttactgc tttagcatcg taccgatcgg caatattgac tgcatccagc
    23461 gtatgctcaa caggaacgcg aatcatggta tctgccagcc attcgacgta atccatatca
    23521 cgggtgattg acggataacg gaagtcgata tccagcacat tcgccagttc atcgaagtat
    23581 tgtttatcga tccctttttc acgtaacaaa ttgaggtagc taaaaatagc cgccacaacc
    23641 tgatcgcgat tcgccagacc tttatcggtt aaagacgcag agatcgctaa tacgccgctg
    23701 ttgccgttga cgataggatc ggagttggcg ctaatgccct caactaaccc ctgcttttgc
    23761 agccagtcag acagtgtacc tgggctgcga ttaccaatca gataggtaat caattcatcc
    23821 gttttactgc ggaacttcgc tgagttgtta tcgatgcgaa actcaacgcg cagaacttta
    23881 cgcggcagcg ctgggacgta atgaatgata atgccctttt gcgcgtcggt gactaccggc
    23941 acggtgattt ccggtttttt gctctctttg ttcggcacgc gaccaaaagt gtccgccgcc
    24001 atttttgcca actccggcag cggtttatta ctgtaaataa ccgccttcat caaattggcg
    24061 gaatagtact tctcgtggaa atctttcagc gcctgctgca ccggattacc aggtttgtcg
    24121 cttaaagttt cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg gttaatggtt
    24181 tctgcgctga cctgtgccat gcgcatcccg tcacgtgtac gcgccatggt taattcagcg
    24241 ttcactgcat tacgctcacg ttcggcgtat ttcttgtcga gcaagggttc tgcaatagca
    24301 tcggccaggc ggtctaccgc accgggcaag gcgtcgttct caacttccag atagaaagcc
    24361 gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt gcattttgag atattcggcc
    24421 agactgtcag cctgcgggta ctttttcgac cccatcagac tcatatgttc aaggtaatgt
    24481 gccagcccct ggtacgcctc gggatcttcc agcgacccaa cgggcaccac cagcgccgat
    24541 agcgatttaa ctgcctgcgg atcagaaacc agcaagacca ccataccgtt atccagacgt
    24601 atagcctgat actggcggtt atctttatca cttttacgga tggtttcctg aatcggctgc
    24661 catcccgttt ctgcctgact taagggtgcc caaagggcaa ctaacaacaa taatgctttg
    24721 aaccaggtgc tgcggggcat tcacggacct cataatcaac tttattttct ggccagattc
    24781 aacaggttaa cgcaaatcat ctgccagaat ttaatcttgt gctgcacgag tcagcctatg
    24841 tttatataac catcagtccg tgactggtgc gcatcataaa gtaagcggat agattgcgca
    24901 atttttatac agcgctcatg actgattaaa gcgaaacagc ggtaacagga aacgttgcga
    24961 ctgttcaacg attgcctcca ttgtctctgg tgttaattgc cgccagagcc tttgatacca
    25021 gatatcatca ccttcgccac tcaccatcat gttgccttcg taagcctgaa ggaatttcgt
    25081 acgggctttt tgcaacgtgg aatcgtcatc cagcatggca tcgttttgcg cgtcataaca
    25141 ggtttttagc cacgcgccgc cactttcagg taacaccagc aatggcgcgg acatcccttc
    25201 acgatacccc tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg caagcggcgg
    25261 aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt tcaccattac caccgctggc
    25321 acagtagaca aggtgttcca gccaaagttg cattccctgc gccacactta ataaagaggg
    25381 acgccagcgc aacaagccat ccggctgcac ctgcggcaac cagccagtta tctgcacacc
    25441 gttgcaagcg agatcaattt ccatactctg ccccggctgg cgacaggcaa tgactctgtc
    25501 ggcaagctgc tgcatctcct ggcactgtgt ttcccagaaa atttcaccaa aagcgccata
    25561 cggtaaatcc cctgccgccc ggaagcggcg gaacaagcgt tcggcatcat cctgctcaac
    25621 cagtgcattc aataactgct gattgatttg ataacggcta agtccttcca gaataaatgg
    25681 ctcggtgtcg gggatttcgc tgtcttcagt acggaagttc acctgcaaac gcatctggaa
    25741 gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt tccagcggca cggtttccgg
    25801 taaggtaaac ggcagcggct gaacaaattc agaatgtgct ttaccagcct ggctggccgc
    25861 aggtagccat tcacgagcat agctttgtcg ttcgcctggc tggtagtttt gcggatcaaa
    25921 cggcatccgg gtatggaggc aagtaagatg cgcttttacc cttgcctcgc tttcatcaca
    25981 gttgagcgct tcatcgcccg gtagataatg actttgcccg atgtagtcga tcagttcctg
    26041 caccagtacc gacgggaaac gttcactgtt atcctgaatg gaacgcccga tatagctgat
    26101 atagagtttt tgctgcgcgg aaattaacgc ttccaggaac aggtagcggt cgtcatcgcg
    26161 acggctacgg tcgccacgct tcggtttctg gctcatcagg tcaaagccca atggcgcaag
    26221 ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa accactttga acggaattga
    26281 acgcattggc atcagagtac aaatgttaac cggcccggcg agaaaacgct ggctgatacg
    26341 ttcttgatcc aggcgctgtg ccagttcatc acgcaatagt gacagcggca ccgcatcgcc
    26401 atactgcgca cctaaacctt cggcgataat cgcctgccat tgttgttcga tcagcgtcat
    26461 cgccgcttcg gtttccgcat ccggcaggaa gagggcgttg agcatatcgc gacaaaccgg
    26521 caaccactct tccagcggac gctcctgtgc cagcccgcga cgccagatgt ttagctgcat
    26581 tagcagtgaa gccagatgcc ccaccagttc tgcaattaag ccgctcgatt catcataagg
    26641 tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg tagcccaaca acatacgcgt
    26701 caggccaaat cgccaggtgt gttgtccggt ggcggggagt tccagctcgc gaacgttgtc
    26761 gtcatctatc ccccaacgaa tgccggattc gttgacccac tgacgtaaat aacgcagccc
    26821 ttcttcggtg atgtcaaacc gcgccgccag caccggcaca tccagtaatg ccagcacgtc
    26881 ttccgacaca aagcggctgt ctggcaacga taacaggctg ataaacgctt caagtacagg
    26941 atgcgactgc cgcgcccgac ggtcggaaat ggcgtaaggc aggtaacgat ccgcaggtgc
    27001 gctaccaaac acagcctgaa taaacggact gtagctgtcg atatcagcca ccatcacgat
    27061 gatgtcgcgc ggagtaagtg tcgggtcttc ttccagcatc gccagcaggc gatcgtgtaa
    27121 aacttcaact tcacgctgcg ggctatggca aacgtggaag gtgatactgc tatccagtgg
    27181 atcaagcgga cgtttgttat cgctacggga aaactcttcg atgttcacac cagcaacggc
    27241 gcggttttcc agttccagaa tgtcagactg aatgttatgc agcaggttat ctggcgtcac
    27301 atcgacaaag gcgtccagct cctggctgct ctccaggtca gaaaggagat aaatgtagtc
    27361 gcgcccaagc ttgccccatg aggccagcag cgggttgccg acatcctgtt caccatcgct
    27421 gttaaagagc tgcccggcat tttcgctgtc gcgaaatagc ggtaattcgt gatcttcaaa
    27481 actgtgtcgg cgctggcgag tcagtagttt cgccagataa gcaggatctt taatgtcgcc
    27541 ccagtaataa cggcaggggt tggtaaacag gagatggatt tcaatatgtt tacccagcgc
    27601 ctggagcgcc tggagataaa caggcggtaa cgcggaaata ccgcatataa agacgcgcga
    27661 aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc gctgatagag
    27721 attggcgcgg tgccagcgcg gttgtccgag ttcatgggta tattccacca gcgccttcca
    27781 caacggcgct tgccaggcct gtgcctctcc cagcccttca accaaatgtc ctgtttccca
    27841 ctgtgccagc cagtccggac gatagaccag atactggtca aacaggtccg ccgcttttga
    27901 ggaaagctgg aacagctttc gcttgtcgct atcgtcagtc agataatgcc gcaacagggt
    27961 aaagtcttcg cgctccagca attgcggcag cagagtcatc agtttccagc tcatgctctg
    28021 tttgttaaag gcgctctctt tggggatctc cggtaacacc cggacgaaca tatcccagat
    28081 aaagctcgca ggcagcggaa aatcaatgtt tgccgcaata ccaaactttt gcgacagggt
    28141 catttgcagc cactgtgcca taccggtact ttgcaccaga atcatctctg gttcgaaagg
    28201 atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac gcttccagca cgtccagacg
    28261 attggaatgg tagaccctta acatagcggc tcctgactac tgacgattcg gacaatgcag
    28321 gcgggtcatc tcgcctgctc tgcccccggg tgaaactagc gtaacgctga tgctgacaca
    28381 tcccgcctgc gatgtctgca ttcggttgac ctgccagttg gcaggtggcg aaatcgcgcg
    28441 caattgcgtt tgctgccaac catgccgcca gagctgttgg tactggtttc tgctggtaag
    28501 actgttcatt aatgttcgct ggaaacccga taacgcagtg acaatcatca ccatcaacac
    28561 catcgccaac attacctccg gcaggctaaa gccttgttga ttcttcaggg aagctgacat
    28621 aacgccccct ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa aacaattttt
    28681 ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt cataaccggc aattaataag
    28741 gcttcattat cttccagtaa acgcaaacaa acccgggcac cagtggcagc gtacagtaag
    28801 cactgaactg cgggctgcgt ctgccaggag tgcattttcc cccacgccaa cgccgactga
    28861 acgatggcct ggcggcgcag tgactgactt tccatgctca cgcgagaggc aaaactgcga
    28921 tcctgctggc tcattccttg taatagcaag ctacccaaaa ccagcagcat caggaccaga
    28981 gccagtgacg aaacaccctt ttcgcggttc acaggttgaa tcctgtcacg ctatagctgg
    29041 catccaccac ggtttgcggt tcagacttac tggcagcacg catattaacc gtcaacaccg
    29101 gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt gtcgataatg atggcatccg
    29161 gattagtgac tttatcccag cccttacctt cacaggatgt cgcaccgcgt agcgtttcca
    29221 acacttgctc cttcagacga aatccaatct ggtcggactc ttttaccggt tcgcgatccc
    29281 agataccgtt actgttcgca tcccactgca caatgataca gtcaccctgt ccgacaattt
    29341 ccagcccttc gccggtacag atgccatgac aataacccgc cctctggaga tgcttcgcga
    29401 cggtaaatac ccgcagccag atttcatctt ccagtgccag cttacgggtg ctcgttaaac
    29461 tttcacgctg taacgcaggc agaaagcgtg cagcccccaa caacaatacg ctactgatcg
    29521 ccatagcaat caacacttcc agcagagaaa aaccttgctc ttttacaggc atccttctgt
    29581 ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa accaccagcc accactcgcc
    29641 cgttgagttt ttgaagccaa tatgcccggc ccatgcggta ttgcgcaggc caaagaaagc
    29701 aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa agaccaatgg
    29761 tgaactgcca tgacaggtat tcgccccagc aacggaactc acaaggcacc ataacgtccc
    29821 ctccctgata aaactgatac tgtggtcgcg gttatgccag ttggcatctt cacgtaaata
    29881 gagcaaatag tcccgcgcct ggctggcggt ttgccaaagc cgttgcgact gctgccagta
    29941 ttgccagcca tagagtccac ttgcgcttag catgaccaaa atcagcatcg cgaccagcgt
    30001 ttcaatcagc gtataaccac gttgtgtttt catgccggca gtatggagcg aggagaaaaa
    30061 aagacgaggg ccagtttcta tttcttcggc gcatcttccg gactatttac gccgttgcag
    30121 gacgttgcaa aatttcggga aggcgtctcg aagaatttaa cggagggtaa aaaaaccgac
    30181 gcacactggc gtcggctctg gcaggatgtt tcgtaattag atagccaccg gcgctttaat
    30241 gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg aaacggtagt cgaagatgga
    30301 ttcgggttta cgtttgataa tcaacttcgg cagcggacgc ggttcacggc ttaattgcag
    30361 atgagtttga tccatatgat tactgtacag gtgcgtatcg ccaccggtcc agacaaaatc
    30421 acccacttcc agatcgcact gctgcgccat catatgcacc agtaacgcgt agctggcaat
    30481 gttgaacggc aggccgagga agacgtcaca ggagcgctga taaagctggc aagagagttt
    30541 gccgtctgcc acatagaact ggaagaatgc atggcacggt gccagcgcca ttttatccag
    30601 ttcgcctacg ttccacgctg aaacaataat gcggcgggaa tccgggtcgt ttttcagctg
    30661 gttcagtacc gtagtgatct ggtcaatatg acgaccatct ggcgttggcc aggcgcgcca
    30721 ctgtttacca tacactggtc cgaggtcgcc gttttcatcg gcccattcgt cccagatggt
    30781 gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc tgcagaaacc acagcagttc
    30841 atggatgatg gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc catcctgcag
    30901 gttaaaacgc atctgatgac caaaaatgga aagcgttccg gttccggtac ggtcgttttt
    30961 ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa tactgtttca tggttcctca
    31021 ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc atcatgatca cacccgcgac
    31081 aatcatcggg atggaaagaa tttgccccat gctgatgtac tgcacccagg caccggtaaa
    31141 ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga aacgcgccgt agccaatcag
    31201 gaacaagcca gacacggctc ccatcgggcg cggtttacga atatacaggt tgaggataat
    31261 aaacagcacc acaccttcca gcagcagctc gtaaagctgt gatgggtggc gcggcagcac
    31321 accgtaagtg tcgaaaatgg attgccactg cgggttggtt tgcagcagca aaatatcttc
    31381 tgtacgggag cctgggaaca gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa
    31441 ttcaccgtta ataaagttgc ccaaacgccc ggcaccaaga ccaaacggaa tgagtggtgc
    31501 gataaaatca gaaacctgga agaaggaacg tttagtacgg cgggcaaaga taatcatcac
    31561 cacgataacg ccaatcaggc cgccgtggaa agacatgccg ccgtcccaga cacggaacag
    31621 atacagcgga tcggccataa actgcgggaa attgtagaac agaacataac caatacgtcc
    31681 cccgaggaag acgccgagga agcccgcata gagtaagttt tcaacttcat ttttggtcca
    31741 gccgctgccc ggacgattcg cccgtcgtgt tgccagccac attgcaaaaa tgaaacccac
    31801 cagatacatc aggccgtacc agtgaagcgc cacgggtcct attgagaaaa tgaccggatc
    31861 aaactccgga aaatgcagat agctactggt catctgtcac cacaagttct tgttatttcg
    31921 ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa
    31981 taatagcaca aggggacctg gctggttgcc ggataccgtt aaaagatatg tatatgatcc
    32041 gcgctataac cctccgcgaa tcagcccgcc catgccgcga cgctccataa aggctgcaac
    32101 ctgatggcga acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa gattttctgc
    32161 ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc gctacagaac gtccgttcat
    32221 agacagatgg cgatacccaa gcccaatgag gattgccacg cacatgggat cgcccgccat
    32281 ttcaccgcac aaacggagat cgattccatg tatttccgct tcccgggcga tcatcgccag
    32341 agctcgtaac attgcaggat gaagactgtc ataaatgttc gccacccggg tattgttgcg
    32401 atcaacggcc agaatgtatt gagtcagatc gttggtgcca acagagatga aatcgacccg
    32461 ctttgccaga tgcggcagca taaataccat tgacggcact tccagcatga tgccgatacg
    32521 tggtttggga atttcgtaac cgatcatctc ctcgacttca cgtccggcac gttcaatcag
    32581 gcggcgtgct tcgtcaactt catcgaggct tgtgaccatc ggcaacagaa tattcaggtt
    32641 gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg atcaagaaga tctccggctg
    32701 atcgagcgta atgcgaatcc cacgccaacc caggcatgga ttctcttcgc taatcggcat
    32761 gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt
    32821 gaacatttgc agcatcccct gatactgcgc cacctgttct tcttccgacg gaaaaccact
    32881 ttgcagcatg aatgggattt cagtgcgata aagaccgatg ccatcaatac ggctgcccag
    32941 tttttcttca tgttccgggc ttaaaccagc attgagcatg acttttatac gctcaccgct
    33001 ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga cggctaagct caatctcttc
    33061 actaattagc cgctgatatt cttgcagcag taccggctcc ggatcgacca gcaattcacc
    33121 gcgatagcca tcgacgatca gcgtccgacg atgcagcacc gaaggctgaa tatccgcgcc
    33181 catcacggta gggatcccca gcgcacgtac catgatcgca gcatgggagt tggctgcgcc
    33241 atctcgcacg acaacaccga ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga
    33301 cagttcatct gccaccagaa tgaaacgttc cggccaggcg ttcggccctt gattagcgtc
    33361 atcaagatga aacagcaatc gctgacccag cgcacgtaaa tcgccagccc gctctttgag
    33421 atagttatcg cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg tttttaccgc
    33481 ccattctgcc accgagcctt tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc
    33541 cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt tctttttgtg caccggcggc
    33601 aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc cggtcagtcg
    33661 ttcgcgttcc agagccggat ccagcgttga tgcctgatac acctgttcca ttaaaggtaa
    33721 cgtggcatcc tgccagcctt cggcaatcgc tacaccaggt gctgccggta atgcgcggat
    33781 tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc gactgagaaa gaatcgctgc
    33841 catctgggtg gcaagcgtca ccaggaagga ttcttcactt tcgtcatact ggcgcaactc
    33901 tcgttgctgt accaccagta caccaagcaa ctggcgacgt tgaataattg gtacgcctaa
    33961 aaacgcgcgg aaacgttctt cttttacgga ggggatgtat ttgaagctgg ggtgcttttg
    34021 cgcatctgca aggtttatcg gttccgccag cctgccaacc aggccgacga tcccttcatc
    34081 aaaggcgagc gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta
    34141 gtaacaacgt cgatcatgat cggccaggta gaccgaacag acctcggtat ccatcgcaag
    34201 acagatgtcg gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc tggctacctt
    34261 ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg acttaacctc ttttacgtcg
    34321 ataagcagat gcgttttgtg gttttggcgt attttcctgc agtgacatca ccacactcgc
    34381 gaactctttc attaccctac ggtagacatc acgtttaaat gacaccacct gtctgaccgg
    34441 ataccagtaa cttacccatc gccagccgtc aaactctggt gtactgctgg tttgcatatt
    34501 gatttctgca tcgccgctca ccagctgcaa gagaaaccat ttttgttttt ggccgataca
    34561 aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca accagttacg
    34621 cgttgaagca aggattcgaa cgtctttgcg gcttaatcct acttcttcaa acaattcacg
    34681 gtacatcgcc tgctctgcgg attctccggg gttgattccg ccttgcggaa attgccagga
    34741 gtgctgacca aatcgccggg cccacattac ctgcccctgg cgattacaaa tcacgatacc
    34801 tacgtttggg cggtagccat cgtcatcaat caccggacta cctcaaaata aagctttata
    34861 tacgaatgat tgtttcatac tccaggaaga cggtaaacca ctctctgcag ggcattacac
    34921 actaacaaca attgaataac tcacagttat gtgcagagtt ataaacagag gaaggggtgg
    34981 atagccgttt ttattcacct tttctgtgga tagagttgtg aagaactacg gaattactac
    35041 gggaaaaccc ggagcattct gaataagccc gatatagaca ttttaaaata ttcattatta
    35101 gtcaatgcat tacatgtaat ttaagtaatg aaaaagagta attcgtgacc caggtcacac
    35161 ctctcattta cgggttggcg aaagatcaac caatgccgta tttatccaca gaatgtgcca
    35221 ctaagttaag cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg
    35281 gaaacagtag tggaggtttt tcacagttat cccagctttc tgtggataac atggtgtaag
    35341 atcctgttta ttttcagtga ccagatttgg aaaacccgtt gcagtgttgc gcaactcgat
    35401 taccggcaac ctaaaaagca atataaatca gataattgaa caacgcatgt ggaaaaagtt
    35461 acactgcgaa tattcggcac ataattgctg tttgtttttt aaccaaggta tcatgacatg
    35521 tcccaacctc gcccactgct ctctcctccc gaaactgaag aacagttgtt agcgcaagca
    35581 cagcaacttt ctggttatac attgggagaa ctggcggcac ttgccgggct ggttacgcca
    35641 gagaatttaa aacgcgataa gggctggatt ggcgtgttac tggagatctg gctaggtgcc
    35701 agcgcaggga gtaaacctga acaagatttt gctgctctgg gcgtggaact taaaactatc
    35761 cctgtggata gtcttggtcg tccgctggaa acaacattcg tttgtgttgc cccgttaacg
    35821 ggcaatagcg gagtgacctg ggaaaccagc cacgtgcgcc acaaactcaa acgcgtactg
    35881 tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt tggatcaccg
    35941 ttgctgtgga gcccgaatga agaggaagac cggcagctac gcgaagactg ggaagaatta
    36001 atggatatga ttgttctcgg tcaggttgag cggattaccg ctcgtcacgg ggagtattta
    36061 cagatacgac cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg tgcacggggc
    36121 gaacggattc tgacgctgcc gcgcggcttt tatttgaaga agaatttcac cagtgcacta
    36181 ctggcccgtc attttctgat ccagtagcca tcgctttgac ctgccgcttt ccgggcatat
    36241 aattaccgct tcattttttt ggcagggctt tttagatgtt atttgcatgg ataaccgatc
    36301 ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga gatcgttctt gggatcgaca
    36361 atattatttt cctttctctg gtggtggcaa agctccccac agcacaacgt gctcatgcgc
    36421 gccgtctggg gttggcggga gccatggtta tgcgtctggc gctgctggca tcaatcgcct
    36481 gggttacgcg cctgacgaat ccgcttttta caatattcag tcaggaaatt tctgcccgtg
    36541 acttgattct gcttctgggt ggcttgttcc ttatctggaa agccagcaag gaaatccacg
    36601 aatccattga aggtgaagaa gaagggctga aaacacgcgt ttcatcattc ctcggcgcta
    36661 tcgtgcagat tatgctgctg gatattatct tcagcctcga ctcggtgatt accgctgtgg
    36721 gtctgtcaga tcacctgttt attatgatgg ccgccgtggt gattgccgta ggcgtgatga
    36781 tgttcgccgc gcgctcgatt ggtgattttg tcgagcgcca tccttcggta aaaatgctgg
    36841 cgctctcttt cctgattctg gtgggcttta ccctgattct ggaaagtttc gacatccacg
    36901 taccgaaagg ttacatctac ttcgcgatgt tcttctctat tgcggttgaa agcctcaacc
    36961 tgattcgcaa caaaaagaac ccgctctgat actccgtacg ctctcctgcg ggagggcgta
    37021 ctcacttctg ttaccctcac agtttaagat tttcctgctt tcaagatata taacgtcagt
    37081 ttataaacag actattatca taggtgggcc aaatgaggat aaccggatga aaaaatgggc
    37141 agtaataatt tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca gtgattacgt
    37201 catggcgacc aaagatggcc gtatgatttt gaccgatgga aaacctgaaa ttgatgatga
    37261 taccgggctg gtgagttatc acgatcagca aggtaacgcg atgcaaatta accgtgatga
    37321 tgtttcgcaa ataattgaac gttaacaaat aaggtcagca tccggctggc cttaagattt
    37381 ttctctttcc cttttccctt ccctctgcca tttttatatt ccttatgtcg tgattataaa
    37441 aaggaaacgg ctatgcaata tcaccgtata ccccacagtt cgctggaagt cagcacgctg
    37501 gggcttggca cgatgacgtt tggtgaacag aacagcgaag ccgacgccca cgcacaactc
    37561 gactatgccg tcgctcaggg cattaacctt atcgacgttg ccgaaatgta cccagtacct
    37621 ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg gcaactggct ggcgaaacat
    37681 ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac
    37741 aagggcatcc gcccggatca ggcgctggat cggaagaata tccgcgaagc gctgcatgac
    37801 agcctcaagc gtctgcagac tgattacctc gatctttatc aggtgcactg gccgcagcgc
    37861 ccaaccaact gcttcggcaa actcggttat agctggacag attctgcgcc tgcggtttcg
    37921 ctgctggata cgctggacgc actggcagag taccaacgag cgggaaaaat tcgttatatc
    37981 ggcgtgtcga acgaaactgc atttggcgta atgcgctacc tgcatctggc ggacaaacac
    38041 gatctgccgc gtattgtcac cattcagaac ccttacagtc tgttaaaccg cagttttgaa
    38101 gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg
    38161 ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat
    38221 acgctcttta gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg
    38281 tatgttgata tcgccagacg tcatggcctg gcccctgctc agatggcgct cgcgtttgta
    38341 cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa
    38401 actaacatcg aaagtttgca tctggagtta agcgaagacg tattagctga aattgaagcg
    38461 gtgcatcagg tttatactta tccggcacca taagattaac gccggttaaa accggcgtta
    38521 aatattaatg acggcgctgc cagatccaca gcgccgttat tgccagcgca aatagcgcac
    38581 cgaagccaat gccaatgggc acgaccggga tgcctaccat taccgccagc gagtaaatgc
    38641 ccagcatcaa caacatggcg ctgttttcgc caaggttttg tactgcaatc gcattccccg
    38701 ccccgacgct ttttttaccc cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc
    38761 cccccaacac gccaatcagc atcaacaagg cataggctgg cagctgctcg tgttgcaggg
    38821 agaaaatcag taccaccacg ccaatcaaaa ttccggctgg catacagcgt gacacggttt
    38881 ccagcgtaac cagcttcgct gccgcacctg cgccaaccac gataccaatc gctaccatcg
    38941 cgttgagata ggtcggcgta gcgttatcgg taatgcccag cgccaccggt acccacagca
    39001 ccaacaggaa acgcagcgtg acacccgctc cccagaataa actggtgccc accagcgaaa
    39061 aacgcgtttc accattgcgc catagcgagg tgcaggcatt caggaaactg cgggtcatgt
    39121 tgatgagatt ccaggactgc cccggacgcg ccgccgccag tttgggaatg tagatattgg
    39181 caacgaccgc accaccgtag gccagtgcgc atgcggccag ggcgaccagg acatgccagt
    39241 cagccagcac accaccggct acggaaccga gcaaaatcgc cgctatggta gaagcttcca
    39301 ttaaaccgtt agcttttact aacttactac ccgtggttaa ttcgccgaga ataccgtatt
    39361 tcgccggtga ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga
    39421 taccaaagca gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc
    39481 ggcctttggc gaagctatcc gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac
    39541 ctacaaacac catttgcagg atgggctggc tccactccgg atagaactgc gctttcagta
    39601 acgccagagt ggcaaacagt agggcattat cgccaaacgc agagagaaac tgcgccacga
    39661 taaccgcttt catccccttc gaccacaacg aagtgttagt gtgcactgac tcactcatcg
    39721 tgttgttcca cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca
    39781 agtaatggca tctgtttcaa atagcgaata tcacgcggta cagcaagctc cggcacgccg
    39841 tgctcacggg catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc
    39901 accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc
    39961 gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg
    40021 gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa acgcacaata
    40081 tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact
    40141 tccagtacac ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt
    40201 tgcaggcgtc cgccctcttc gataccaggg accgacaaca ggcgcgcatc cattcctggt
    40261 agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc
    40321 gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc caaatttatc ctgccaaagc
    40381 tgtttggtac tttcttgtag tttttctgcg cctgccacca catagcgtag acgatagaag
    40441 tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag tcgaagtgcc gaacaacacg
    40501 gtgcaactgc ggtcatacac caactccggc acaatacggt aatgcagcgg gcttggataa
    40561 agaaacactt ctgcacctgt gagcagtggc gtaaacaggc ctaccgtcag cccaaaggag
    40621 tgaaacagcg gtaacgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta
    40681 atctgctcga cattcgccag aatgctttta tggctatgga cgacgccttt tggatggcct
    40741 tcagaaccgg aggtaaaaag gatcagcgcc tcttcttccg gctgctgttt aacctgtgcc
    40801 agacgcggca tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct
    40861 tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt
    40921 ttgcctttat cgagaaactg gcgggaagtg aagatggttt tgatttcagc cgccgtaata
    40981 gcactggtca gcccttttac cccagcagtg tagttcatca ttgcgggaat gcggcgacgg
    41041 gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg
    41101 cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc
    41161 aatttgcgat aggagtctgg ggtaaaattg acgtcttcga cacatttctt ccctgctccg
    41221 aagcggtaca ttgcactcag taaagattcg tacagcgttt cacgcgggcg caccgccatt
    41281 cgcgcttcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca
    41341 cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg
    41401 aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca
    41461 atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaaacccgc gccatcgtag
    41521 attttcatca gcgagcctgt ggtggtgatg cgtccttcag ggaaaatcac cactggtcgg
    41581 ccctgttcca ccagacgtac cagatgttta atcgccatag gttgcgtcgg gtcgagagga
    41641 acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa
    41701 acggcaaaca ctggacgcac aggtaaaaac agtccaagca aaatgccatc aataaaagag
    41761 acgtgattag gcgtaattag aacgcgctcg cccttcagtg cctgggggtc acccgtaacg
    41821 cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc
    41881 tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg
    41941 acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg
    42001 gtacgaagag cgtaccgaat aatctcacca atcaatacct ctgggatctt gattgtggtc
    42061 tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt
    42121 gtgtaaattg tcggttactg ttacagattg atgaccggca aaaaaaaacc tgcgcatctg
    42181 cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac ttcacctatc aatacctctg
    42241 ggatcaccac tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt
    42301 cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc ttacttattt gcgtttggct
    42361 cacattccca cgatgaaaac acgccgcccc ttgaaccaac gggcgttttc cgtaacactg
    42421 aaacaatgta agcgtttacc cactaaggta ttttcatggc gaccataaag gatgtagccc
    42481 gactggcagg cgtttcagtc gccaccgttt cccgcgtcat taataattca cccaaagcca
    42541 gcgaagcttc ccggctggct gtgcatagtg caatggagtc tcttagctat cacccgaacg
    42601 ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt cggtctggtc gttggtgatg
    42661 tttccgatcc gtttttcggt gcaatggtga aagcggtcga acaggtggct tatcacaccg
    42721 gtaatttctt attgattggc aatggttacc acaacgaaca aaaagagcgt caggccattg
    42781 agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg
    42841 ccgatttagc ctcattattg aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc
    42901 ctggctttga aaaccgttgt attgctctgg acgatcgtta cggtgcctgg ctggctacgc
    42961 gtcatttaat tcagcaaggt catacccgca ttggttatct gtgctctaac cactctattt
    43021 ctgacgccga agatcgtctg caagggtatt acgatgccct tgctgaaagt ggtattgcgg
    43081 ccaatgaccg gctggtgaca tttggcgaac cagacgaaag cggcggcgaa caggcaatga
    43141 ccgagctttt gggacgagga agaaatttca ctgcggtagc ctgttataac gattcaatgg
    43201 cggcgggtgc gatgggcgtt ctcaatgata atggtattga tgtaccgggt gagatttcgt
    43261 taattggctt tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc
    43321 gttacccaat cgtgacgatg gcgacccagg ctgccgaact ggctttggcg ctggcggata
    43381 atcgccctct cccggaaatc actaatgtct ttagtccgac gctggtacgt cgtcattcag
    43441 tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag cgactaaccg cagttaaagc
    43501 aattccagcg ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca
    43561 ttatcaaaca gaacttctgg taacagcgga cggctattgt agttggatga cattgatgcg
    43621 ccatatgccc ctgtatcatg cagtaccaga taatcacctg ccttcacttc cggcaaggcg
    43681 cggggttcaa catttccccc ttcctgctgg gtaaagacat cgcccgattc acataacggt
    43741 ccggcgacga cggtttccac cgttggtgcg tgttccagag aacgaccatc agctgccagg
    43801 gcactgatat ggtggtaact accgtacatt gccgggcgca tcagatcgtt aaacccggca
    43861 tcaaccagca caaagtggcg gctacccatt tgtttgacgc tccgcacctg agtaattaac
    43921 acgccagact gcgctaccag gaagcgaccc ggttcaattt ccagtttcac agggtggccc
    43981 aaatggcggg cgatttgctc acgcgcggca ttccacagac cataataatg ttcggtatca
    44041 accgcctctt caccctgttg ataaggaata gaaagcccac cgcccgcaga aatagcctgt
    44101 aaatcctgac cgaattcgat gacctgacgc accatagcac cacacacctg ttccagatgg
    44161 gcataatcaa cgccagaacc aatgtgcatg tgaatgccga ccagctgcag atgatgacgt
    44221 tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt gctgttttcg
    44281 ccaccggtat tggttttttg gctatgtccg tgaccaaacc ccggattaac gcgcagccat
    44341 acccgatgcc ctggcgaaac ctggcccagt tggtcgaaca tatcaacaga acccgcattc
    44401 accggaattt gcaattcact gacgcgttca agcgtcgcct gatcgataac atctgccgta
    44461 aaaacaatat catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt
    44521 tcgcctaacg agacggaatc cactttcacg ccctgctcac gcattaagcg caaaatatga
    44581 atattggaac aggctttctg tgcaaagcgc accacatcaa actgtttcag cgctgcaatc
    44641 tgccgacgaa taatttgcgc atcgtagacc cacaccgggc agccaaattc agcgggcaaa
    44701 cgcagcagat tttcggcggt gagatcggta tcggtgctga acagtgaatg tggcataaca
    44761 aactccagat gagtgctttt ttatgattac gccacatcat aaaaagaata aaaaatatcg
    44821 atttatgtcg agtctatgca aaaatgatat ggattaccgg attgcgagag agcgctaatg
    44881 gccgccgtta acttacgtca tattgaaatt tttcatgcgg taatgaccgc cggaagcctg
    44941 actgaggcag cacacctgct acacacctca cagccaaccg tcagccgcga actggcgcgc
    45001 tttgagaagg tgatcgggct gaaattgttt gagcgcgtac gtggacgatt acatcctacc
    45061 gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct ggtacggact ggatcgcatt
    45121 gtcagcgccg cagaaagtct gcgcgagttt cgccagggag aactgtctat tgcctgcctg
    45181 ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac cctttctggt acgttatccc
    45241 gatgtcagct taaatatcgt gccccaggaa tcaccgctac ttgaagagtg gctctcggcc
    45301 cagcgtcatg atttaggact cactgagaca ctccatacgc ctgcgggaac agaacgtacc
    45361 gaattactct ctttagatga attgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa
    45421 aaggtattaa cgccggatga ttttcagggt gagaactaca tcagcctttc ccgtactgac
    45481 agctatcgcc agttgctgga tcagctattt actgaacatc aggttaaacg acgcatgatc
    45541 gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac gggcgggggt aggtgtttcg
    45601 gtggttaacc cgctcaccgc actggattat gcggcaagcg gtttagtggt gcggcggttc
    45661 agtattgcgg ttccgttcac cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg
    45721 ctggtgcagg cgtttagtga gcatttacaa gcggggttac cgaaactggt cacttctctt
    45781 gacgctattt tgtcgtcagc tacgacagca taaaagcgac agcatcctcg gcatggatcg
    45841 ccgcagtatc aaacacaggc aaaacactgc gctcttctgg caccagtaaa ccaatttctg
    45901 tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat
    45961 aataagcgcg tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa ataatctgat
    46021 taattttcgc ccgttcatcc gcttcaggaa taagacaatt gatggaaaat tgttccgtca
    46081 gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc gccacacgag
    46141 tcattccggc cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag
    46201 tgcaacgtga ctcaatggca tccgccactt tatgcatcgt attggtacat aacacaatac
    46261 cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc ccggttttat
    46321 cccattcccc gcgacgctgg cactcttcta tttcatgaaa atcgacgcta tgtagcagca
    46381 cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc ttcatttatc agacgatagt
    46441 aaggaatggt ggattcccag ctcattcctc ccagcaaacc aattgttttc attcctctct
    46501 cctgatgtgt gttaccgcaa caaggctgaa taaggaacga gacaaacgcc tcaacggcca
    46561 ggtgcccgat ctctattaac gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa
    46621 tcagacgccg atatttctca acttctcgcc tgccatcagt ttgcgttcga tatgttccag
    46681 cgtgacattt ttggtttccg gaatgagcca gaaagtgatg cccacaaacg caatgttcag
    46741 cgcagtgtag agccagaacg taccggcagc gccaatgcta tcaagcagtg tcaggaaggt
    46801 cgcgccgata atcatattcg acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc
    46861 gcggcatttc agcggctgaa tttcagagca caggatccac accactggcg cggcgctcat
    46921 cgcataaccg gcaatacaca tcatcgtcat gccaacagag agccaggaca agccactgga
    46981 agccgtaccg ttatcaaact gcatcaggca atagcccagc accagagtgc ctaacgccat
    47041 cacgctgaaa ccaattttca gagccggttt acgacctgct ttatctaccg taaagaccgc
    47101 aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc
    47161 tgtggtcgta aagcccgcca ttttgaagat acgcggcgca tagtacatga tgatgttcat
    47221 accggtaaac tgctgcatcg cctgcaacaa cataccgaga aacacagcac gacggacgtt
    47281 acggttgatc ttaaacagtg cccaaccgcc ttgttttaac ttcaggcttt cacgaatttc
    47341 gttgagttct tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca cttcttccgc
    47401 ctcaatatga cgcccctttt ccgccagcca gcgcgggcta tttggcagga agaccaccag
    47461 aataatcagc aggactgctg gtaaagcaag aacccccaac attgcgcgcc agttaccgct
    47521 ataactgaac gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg
    47581 gtacatactg atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg
    47641 agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc acacgagcgg cgattaacat
    47701 ctctacgctg gtcgcaaaag cggaccctat agaaccgagt acaaacagga tggcccccgc
    47761 catcaggctg tatttacgcc ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat
    47821 tgctgcgccg agcatcatgc tactaaccac ccattcctgc aaacgactgg tcagcacaaa
    47881 gtgatcggta atgaacggca acgctccggc gattacgccg atatcaagac caaataacaa
    47941 tcctgcgacc gcagcagcta ccgaaacaaa catattcata cgccgcgtat cacgcaaaga
    48001 acgtggcgtt aaagcagatt ccgtattgat agtaaccatt ttttcctgcc agcagagagt
    48061 aagacatagt gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga aacagcataa
    48121 atatggatta aattgctgcg acatgccgtt atgtgatgga tattccaatt ttaaaattaa
    48181 gttgaattat tgagattatt attaaccacc taattttaaa gcagataaaa ttcataaagt
    48241 tcattaattg ataattgata tggattattt cataaccatg atatggatta tgatgatcta
    48301 gagatataaa aaaccctgcc atgcggcagg gtcataaaag taagaggaat gaattaacgc
    48361 gccagccaac cgccatccac agcaatggta taaccattta cataatctga agcgctggag
    48421 gcaagaaaca ctaccggccc catcaggtca ctcggcagtc cccaacggcc agctggaatg
    48481 cggtcgagaa tttccgcgct acgttgttca tctgcccgca gttgttgggt attgttggtc
    48541 gccatgtaac ccggagctat cgcattaaca ttaatgttgt gtttagccca ttcattcgcc
    48601 atcaatcgcg tcacgcccat cacgccgctt tttgatgcgg tataagaagg cacacggatc
    48661 ccgccctgga aggagagcat tgacgcgata ttgataatct tgccgccatt gccttgcgcg
    48721 atgaagtgtt tcgccgctgc ctgagacatg aagaatacgc tcttgatatt caggttcatg
    48781 acatcgtccc agtccttttc gctgaactcg agagcatctt cgcggcgaat caatccggcg
    48841 ttattcacca ggatatcaat atgaccaaac tcagctaccg cgcgatccag cagtccagga
    48901 ataccatcaa tctttcgcag atcggcggtc aggcttaaaa aacgacgccc cagcgccgtg
    48961 acctgcttga tggtttcagt cggttcaacg atgttaatgc caacaatgtc acagcccgct
    49021 tgcgccagcc ccaacgccat cccctggccc agcccagtat cacaaccagt gacgaccgca
    49081 actttacctt cgagagaaaa tgcacttaaa atcataacaa taccttattc gtttatgccc
    49141 acaactagcg caaatcttta acggccacat ggtccatatc atcaaagacc tggttttcac
    49201 cgaccatgcc ccagataaag gtataagctt tggttccgac gccggaatga atcgaccagc
    49261 tcggggagat caccgcctgc tcgttatgca tcacaatgtg acgcgtttct tgcggctgcc
    49321 ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa atagaaataa acttccatcc
    49381 ggcgctcgtg ggtgtgacac ggcatggtgt tccacaagtt acccggagcc agctccgtca
    49441 gccccatact caattggcag gtttccagca catccgggac aaaatatttg ttaatcgtgc
    49501 gacggttact ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc ggtgtgactt
    49561 ttttggtagg atacgtcgta tgcgcgggtg cgcaattgta ataaaacttc gccggagtgc
    49621 cggtatcaat actagcaaag acaacttctt ttgcgccttt acccacatac agggcgtcgc
    49681 ggtgaccgat ttcatagcat tgaccatcga cagtaatcgt accggcaccg ccaatattga
    49741 taacacctaa ctcgcgacgt tcaaggaaat aacttacgcc gagttgttta cccacttccc
    49801 cgccaacgga aaccgtttta gttaccggca taatgccgcc aataataata cggtcaatgt
    49861 ggctgtaaac catggtgtac tcatcggcga caaatacctt ttcaaccaaa aattcattgc
    49921 gcagcccctg ggtttccagc gtttttgcgt gcgcactgtg gatgctctgt cttacgtcca
    49981 catcaacctc cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc
    50041 gttttctcta taaggggatg atatgcccat gggatcgggt tttcaattaa aaataaaaca
    50101 atgtttcatt tttaagttaa ggattaaaaa aagtgcagtg tcgatcacga atctgtcatg
    50161 agaatggaaa aatcagggag cccggaaaat gtatgtcgac ttgccagcca tcattattca
    50221 gcagattaat gacggcagat atttatcggt aaggaggaaa tgcagagcta ttcgtcacgt
    50281 tcaatggtca atgccacacc ctggcccccg ccgatacaaa gcgttgccag tcctttgcgg
    50341 gcattacgtt tcaccatttc atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct
    50401 atcgggtgac cgagtgcgat cgcgccacca ttgacattga cccgacgctc atcccactca
    50461 agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt cattagcctc gataagatca
    50521 acatcagcca actgccagcc tacacgctcc aggcaacggc gggtcgcata caccggcgca
    50581 attcccatca atgccggatc tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg
    50641 ggtaaattca acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg
    50701 tttatggatg atgcattacc cgctgtcaca gaaccgagac tatcaaatga aggatttaaa
    50761 cgggctaaac cttctgcact ggcgtcagtg tgtggctgtt catcggtatc aacaaccaac
    50821 gtctgaccgt tactttgggt cattaccggg acgatctcat ctttaaatcg tccggcgtca
    50881 atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ttgacggctg
    50941 atgccatatt cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca
    51001 tcccataacc catcatgcac aagactgtca accagctggc tattgccaag ctgtgcaccg
    51061 gtgcggctat cagtcagaac atgtggtgcg cggctcatgt tttcctggcc accggcgatg
    51121 acaatatcag cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc tttaagcccg
    51181 gaaccgcaga cgtcattaat cgtgattgca gaaacgctat taggcagacc acctttaata
    51241 gccgattgcc ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact
    51301 tcatccaccg catatgcagg aacgccggta cgttctatta acgctttcac gaccagacta
    51361 ccaagttcca cggcggaatg acccgctaac gcaccgcgaa agcagccgat aggtgtccgt
    51421 aacgccccga caatcacaac gtctttcatc acaacctcgc accaattaac agtgcaatag
    51481 taaatgattg ttaattacag ttatcttaat tgtttaaaaa aagtgatttt tatcacaaag
    51541 gaaatatacc tgagcagtag tcagagtcat aactggcacg taaggtttgc aaccactacc
    51601 ccaccaatag aggggtagat agggcgttaa tctcccatac ttaacctggt ttatggtaaa
    51661 ttgccctcca ttttgtttaa tttgtagatg atacgttcag ataatgtcta atatttggtc
    51721 taaagaagaa actctgtgga gtttcgcgct ctacggcaca gccgttggtg caggcacgct
    51781 cttccttcct attcagttag gttcggcagg ggctgtggtc ctgtttatta ctgctctggt
    51841 cgcctggcct ttaacatact ggccacataa agctttatgc cagttcatcc tctcatcgaa
    51901 aacatcagca ggtgaaggga taacgggcgc ggtaacacac tactatggca agaagattgg
    51961 taatctgatt accacgctgt acttcatcgc cttttttgtc gtcgtgttga tatatgcagt
    52021 ggcaattacc aactcactta cggaacagct ggcaaagcat atggttattg atcttcgcat
    52081 ccgtatgttg gtgagtctgg gtgttgtatt aattctgaat ctcatttttc tgatgggacg
    52141 tcatgccact attcgggtaa tgggattttt ggtattccca ttgattgcct atttcttatt
    52201 tctttccatt taccttgtag gtagttggca acctgatcta ttaacaaccc aggtagagtt
    52261 caatcagaat acccttcacc agatatggat atcgattccc gtgatggttt tcgcctttag
    52321 ccatacgccc attatttcta cgtttgccat agacagacgt gaaaaatatg gcgaacacgc
    52381 tatggataaa tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca taagtgtact
    52441 gttctttgtc tttagctgcc tgctttctat tccaccttcg tatattgaag cggctaaaga
    52501 agaaggggtc accattttat cggcgctttc tatgctgccg aacgccccag catggttgtc
    52561 aatttccggg attattgtcg cagtagttgc gatgtcgaaa tcattcctgg gtacgtactt
    52621 tggcgttatt gaaggtgcca cggaggtcgt caaaacaaca ctacagcagg ttggtgtaaa
    52681 gaaaagtcgt gcatttaacc gcgcactatc aattatgttg gtatcgctga ttaccttcat
    52741 tgtttgttgc attaacccga acgcgatttc gatgatttat gcgatcagcg gcccgctcat
    52801 tgccatgata cttttcatca tgcctacgct atcaacatat ctcatcccgg cgcttaaacc
    52861 ctggcgctcc atcggaaatc tgattacgct gatcgtgggt atcctgtgcg tatcggtaat
    52921 gttctttagc taatatccat tgtgaatggt tcaaaattgc tacaattttg aaccattcca
    52981 gttttcataa tgtgcatttg ttattattcc gctctatcac ttctctcgtc atatcctagt
    53041 attatcagtt agttaaacac acaaagagat ctgaacatct taaataagac tatttaagat
    53101 gcataactta gattcgcaag atatcttctg gtcattcaaa aacaatttgc gatatagata
    53161 ttctcatacc atgagatagt tatctgaacc accctcaacc aggtaagatt aaatcagtga
    53221 aacataatca tatttaataa cgagaaatgc attttaaaat gtcttaatca gaattatagc
    53281 aaatacagat taatcgcaat ttttcccagc aaaattacaa aatcatggtg tattgacatc
    53341 atcaatcacc aatgaaatta tgcaattata tacggatagg gaggttctta acatggggct
    53401 ttgtagtcgt tataaaagtc ttacatgcaa tagttgctca atgcattgcc aaataatgcc
    53461 agaagagtca ccgcgtttgc agtactgcgc aaactcgtgt ttttgtatgt ggaccgaaga
    53521 aagttcatat tttaatcgtg gtgtggtaga aggtatctta acaaaaaacc acaatgcgag
    53581 attaagcgga tatatttttg tcgatttttc agtaagtttt ttacgtctat tccttgaaaa
    53641 agactggatt gactatcttg caagtactga catggggatt gttttagtca gcgaccgcaa
    53701 tatgcaatcg ttagctaatt actggcgaaa acacaactct gcaatatctg ctgtcatata
    53761 taatgatgat ggtcttgatg ttgccaatga aaagatcaga caactattta ttggtcgtta
    53821 tttatcattt accagaggta acacattaac tcagatggaa ttcaccataa tggggtatat
    53881 ggtttctggt tataatccat atcagattgc tgaagttctg gatatggata tccgtagcat
    53941 ctacgcgtac aagcaacgaa tcgaaaagag aatgggtggt aaaataaacg aattatttat
    54001 tcgttcacat tcggtccaac attgatactc aacaatctgc cagaatcata ctctgtttat
    54061 acgggcattt attgaaatgc ccgtatttta taaataggcc gtcccctcaa gagtaaacac
    54121 catagtattt aaaaccatca cacgaaaaat tcagaagcat tacgaaatat gaacttacaa
    54181 acaatagtgg cataaatgtt aaccatgtta atttacataa aattttacgt tgcgacatta
    54241 aagcctcatt tcaatcatca tgataaatat aaaattaata tatatttatg ccgtaaatac
    54301 caatatactt agcaaactat gtgatttcca tttcgattga tttagtgttt attgacgtat
    54361 gtactggatt attaacgata atatcgagtt ctggcctgag caccgaaaat taatatcggt
    54421 acataacgcc gatcttaacg tcgttctgac aacgccagcc agtcgttgtt tatcactgct
    54481 acttgaagct tttcctgatg tggttgcaca acaagatttt ttcaccagag tctgggaaga
    54541 agaaggtatg cgtgtgccta ctaacacgtt atatcagaac atatccatta tcagacgcgg
    54601 atttcgcgct gttggtgata ctacccactc gcttattgca acagtgccga gaagaggatt
    54661 caagatccat aatgacatta acatacaaaa tcatgtaata aactcatcaa cagacgcaca
    54721 tacacacaat gccccacctg ccataaaagt taatgcggga tacaaagaga gcattggtgg
    54781 cgcaaagaat ttcaataaca aaatcctcaa acatataaaa agttatctaa ttatgttgag
    54841 cgcatttgtc ataggcgcat attctgcata ttggctatgg aataataatc aaccgaagcc
    54901 atttttcaag gattacaaga ctgttgcgga aattaatggc tgccatttta atgtaacaga
    54961 agatacaatt gatgggttga aagagttcga taaatataaa acacggatac tggattcggg
    55021 gattaactgc aaaaaatatc cgtggttata cttccctctt gcaaaatcct caccggggat
    55081 gattgttatg gcatgcaata aaaactataa ccaacatgaa gtggcaggct gtttaaccct
    55141 gtcttaccgt gaggttaatc gtggttgatt ataaaaaaaa acttattatt tattttagtt
    55201 tttatcagtg ggtttattct cttcactgtc tattcttaca ctgcagaaaa aatgatatat
    55261 aacgaaacat gcactgcaaa ttgggtaatt tttaatgacc agggacgagc aaatcttacc
    55321 atagacttta tgtataacaa aaaaaataaa acaggaactg ttgcgttgag tggaacctgg
    55381 caacaaggta acagagaaag taaatcaata cggcgaaata ttgaatacac atgggttgaa
    55441 aactatgaca cagcccattt aacatcaaag aaagttaata agtttgagat tatggatcaa
    55501 gtcgatgatg atagacttgc agaacttatt cctgattttt atgtctttcc
//
LOCUS       DAZKIF010000012        54858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZKIF010000012 DAZKIF010000000
VERSION     DAZKIF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.012"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            323..409
                     /gene="leuL"
                     /locus_tag="ACHOTW_000796"
     CDS             323..409
                     /gene="leuL"
                     /locus_tag="ACHOTW_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP9842959.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            502..2073
                     /gene="leuA"
                     /locus_tag="ACHOTW_000797"
     CDS             502..2073
                     /gene="leuA"
                     /locus_tag="ACHOTW_000797"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP9842960.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            2073..3164
                     /gene="leuB"
                     /locus_tag="ACHOTW_000798"
     CDS             2073..3164
                     /gene="leuB"
                     /locus_tag="ACHOTW_000798"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP9842961.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3167..4567
                     /gene="leuC"
                     /locus_tag="ACHOTW_000799"
     CDS             3167..4567
                     /gene="leuC"
                     /locus_tag="ACHOTW_000799"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP9842962.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4578..5183
                     /gene="leuD"
                     /locus_tag="ACHOTW_000800"
     CDS             4578..5183
                     /gene="leuD"
                     /locus_tag="ACHOTW_000800"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP9842963.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5291..5422)
                     /gene="sgrT"
                     /locus_tag="ACHOTW_000801"
     CDS             complement(5291..5422)
                     /gene="sgrT"
                     /locus_tag="ACHOTW_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP9842964.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5511..7169
                     /gene="sgrR"
                     /locus_tag="ACHOTW_000802"
     CDS             5511..7169
                     /gene="sgrR"
                     /locus_tag="ACHOTW_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP9842965.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7398..7967)
                     /gene="espY2"
                     /locus_tag="ACHOTW_000803"
     CDS             complement(7398..7967)
                     /gene="espY2"
                     /locus_tag="ACHOTW_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFP9842966.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8209..8308
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8339..9322
                     /gene="thiB"
                     /locus_tag="ACHOTW_000804"
     CDS             8339..9322
                     /gene="thiB"
                     /locus_tag="ACHOTW_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP9842967.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9298..10908
                     /gene="thiP"
                     /locus_tag="ACHOTW_000805"
     CDS             9298..10908
                     /gene="thiP"
                     /locus_tag="ACHOTW_000805"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP9842968.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            10892..11590
                     /gene="thiQ"
                     /locus_tag="ACHOTW_000806"
     CDS             10892..11590
                     /gene="thiQ"
                     /locus_tag="ACHOTW_000806"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP9842969.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(11677..12441)
                     /gene="yabI"
                     /locus_tag="ACHOTW_000807"
     CDS             complement(11677..12441)
                     /gene="yabI"
                     /locus_tag="ACHOTW_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9842970.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12527..13405)
                     /gene="araC"
                     /locus_tag="ACHOTW_000808"
     CDS             complement(12527..13405)
                     /gene="araC"
                     /locus_tag="ACHOTW_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP9842971.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13744..15444
                     /gene="araB"
                     /locus_tag="ACHOTW_000809"
     CDS             13744..15444
                     /gene="araB"
                     /locus_tag="ACHOTW_000809"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP9842972.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15455..16957
                     /gene="araA"
                     /locus_tag="ACHOTW_000810"
     CDS             15455..16957
                     /gene="araA"
                     /locus_tag="ACHOTW_000810"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9842973.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17157..17852
                     /gene="araD"
                     /locus_tag="ACHOTW_000811"
     CDS             17157..17852
                     /gene="araD"
                     /locus_tag="ACHOTW_000811"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9842974.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17927..20278
                     /gene="polB"
                     /locus_tag="ACHOTW_000812"
     CDS             17927..20278
                     /gene="polB"
                     /locus_tag="ACHOTW_000812"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP9842975.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20442..23348
                     /gene="rapA"
                     /locus_tag="ACHOTW_000813"
     CDS             20442..23348
                     /gene="rapA"
                     /locus_tag="ACHOTW_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP9842976.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23360..24019
                     /gene="rluA"
                     /locus_tag="ACHOTW_000814"
     CDS             23360..24019
                     /gene="rluA"
                     /locus_tag="ACHOTW_000814"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP9842977.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            24232..24410
                     /locus_tag="ACHOTW_000815"
                     /pseudo
     CDS             24232..24410
                     /locus_tag="ACHOTW_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(24437..25174)
                     /gene="espY1"
                     /locus_tag="ACHOTW_000816"
     CDS             complement(24437..25174)
                     /gene="espY1"
                     /locus_tag="ACHOTW_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFP9842978.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANLDGATLDGATLDGATHLYDEVIIINKI
                     TPKKIDTEEVATKQSTAEQITDNAIIE"
     gene            complement(25469..26284)
                     /gene="djlA"
                     /locus_tag="ACHOTW_000817"
     CDS             complement(25469..26284)
                     /gene="djlA"
                     /locus_tag="ACHOTW_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP9842979.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26539..28893
                     /gene="lptD"
                     /locus_tag="ACHOTW_000818"
     CDS             26539..28893
                     /gene="lptD"
                     /locus_tag="ACHOTW_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP9842980.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28946..30232
                     /gene="surA"
                     /locus_tag="ACHOTW_000819"
     CDS             28946..30232
                     /gene="surA"
                     /locus_tag="ACHOTW_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP9842981.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30232..31221
                     /gene="pdxA"
                     /locus_tag="ACHOTW_000820"
     CDS             30232..31221
                     /gene="pdxA"
                     /locus_tag="ACHOTW_000820"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP9842982.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            31218..32039
                     /gene="rsmA"
                     /locus_tag="ACHOTW_000821"
     CDS             31218..32039
                     /gene="rsmA"
                     /locus_tag="ACHOTW_000821"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9842983.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            32042..32419
                     /gene="apaG"
                     /locus_tag="ACHOTW_000822"
     CDS             32042..32419
                     /gene="apaG"
                     /locus_tag="ACHOTW_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP9842984.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32426..33274
                     /gene="apaH"
                     /locus_tag="ACHOTW_000823"
     CDS             32426..33274
                     /gene="apaH"
                     /locus_tag="ACHOTW_000823"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP9842985.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(33271..33585)
                     /locus_tag="ACHOTW_000824"
     CDS             complement(33271..33585)
                     /locus_tag="ACHOTW_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFP9842986.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(33588..33821)
                     /locus_tag="ACHOTW_000825"
     CDS             complement(33588..33821)
                     /locus_tag="ACHOTW_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFP9842987.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(33907..34386)
                     /gene="folA"
                     /locus_tag="ACHOTW_000826"
     CDS             complement(33907..34386)
                     /gene="folA"
                     /locus_tag="ACHOTW_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP9842988.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34578..36440)
                     /gene="kefC"
                     /locus_tag="ACHOTW_000827"
     CDS             complement(34578..36440)
                     /gene="kefC"
                     /locus_tag="ACHOTW_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP9842989.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIADCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36433..36963)
                     /gene="kefF"
                     /locus_tag="ACHOTW_000828"
     CDS             complement(36433..36963)
                     /gene="kefF"
                     /locus_tag="ACHOTW_000828"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP9842990.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(37071..38402)
                     /gene="yaaU"
                     /locus_tag="ACHOTW_000829"
     CDS             complement(37071..38402)
                     /gene="yaaU"
                     /locus_tag="ACHOTW_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9842991.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38460..38747)
                     /gene="fixX"
                     /locus_tag="ACHOTW_000830"
     CDS             complement(38460..38747)
                     /gene="fixX"
                     /locus_tag="ACHOTW_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP9842992.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38744..40030)
                     /gene="fixC"
                     /locus_tag="ACHOTW_000831"
     CDS             complement(38744..40030)
                     /gene="fixC"
                     /locus_tag="ACHOTW_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9842993.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(40081..41022)
                     /gene="fixB"
                     /locus_tag="ACHOTW_000832"
     CDS             complement(40081..41022)
                     /gene="fixB"
                     /locus_tag="ACHOTW_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP9842994.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(41037..41807)
                     /gene="fixA"
                     /locus_tag="ACHOTW_000833"
     CDS             complement(41037..41807)
                     /gene="fixA"
                     /locus_tag="ACHOTW_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP9842995.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            42286..43800
                     /gene="caiT"
                     /locus_tag="ACHOTW_000834"
     CDS             42286..43800
                     /gene="caiT"
                     /locus_tag="ACHOTW_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP9842996.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43831..44973
                     /gene="caiA"
                     /locus_tag="ACHOTW_000835"
     CDS             43831..44973
                     /gene="caiA"
                     /locus_tag="ACHOTW_000835"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP9842997.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            45102..46319
                     /gene="caiB"
                     /locus_tag="ACHOTW_000836"
     CDS             45102..46319
                     /gene="caiB"
                     /locus_tag="ACHOTW_000836"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP9842998.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46393..47946
                     /gene="caiC"
                     /locus_tag="ACHOTW_000837"
     CDS             46393..47946
                     /gene="caiC"
                     /locus_tag="ACHOTW_000837"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP9842999.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            48055..48840
                     /gene="caiD"
                     /locus_tag="ACHOTW_000838"
     CDS             48055..48840
                     /gene="caiD"
                     /locus_tag="ACHOTW_000838"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP9843000.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48846..49436
                     /gene="caiE"
                     /locus_tag="ACHOTW_000839"
     CDS             48846..49436
                     /gene="caiE"
                     /locus_tag="ACHOTW_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP9843001.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49555..49950)
                     /gene="caiF"
                     /locus_tag="ACHOTW_000840"
     CDS             complement(49555..49950)
                     /gene="caiF"
                     /locus_tag="ACHOTW_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP9843002.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(50211..53432)
                     /gene="carB"
                     /locus_tag="ACHOTW_000841"
     CDS             complement(50211..53432)
                     /gene="carB"
                     /locus_tag="ACHOTW_000841"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9843003.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53450..54598)
                     /gene="carA"
                     /locus_tag="ACHOTW_000842"
     CDS             complement(53450..54598)
                     /gene="carA"
                     /locus_tag="ACHOTW_000842"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP9843004.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        13120 a        14774 c        14299 g        12665 t
ORIGIN      
        1 attcaccaaa tcaatatcaa aaaatccgca aaacatataa ttcaatacaa atcatcagga
       61 taggttttgc aacgcgtgca ttttgtcccc tttttcctcg tggattagat gcaaaaattt
      121 attctgaaat atgtcaaccg atgaaaagca tcggtagtta agcagaaatt aatatcgctt
      181 gttttaacca cagcagcaca attagctaat cttacggatg cagaactcat gctggcggga
      241 cgtttttatt gcgtcagggt tgacatccgt ttttgtatcc agtaactcta aaagcatatc
      301 gcattcatct ggagctgatt taatgactca catcgttcgc tttatcggtc tactactact
      361 aaacgcatct tctttgcgcg gtagacgagt gagcggcatc cagcattaag ccagcacgca
      421 gtcaaacaaa aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg aggcgctcta
      481 aaagagacaa ggacccaaac catgagccag caagtcatta ttttcgatac cacattgcgc
      541 gacggtgaac aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg
      601 gcccttgagc gtatgggtgt tgacgtgatg gaagtcgggt tccccgtctc ttcgccgggt
      661 gattttgaat cagtgcaaac catcgctcgc caggttaaaa acagccgtgt atgcgcgtta
      721 gctcgctgcg tggagaaaga tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc
      781 ttccgtattc atacctttat tgccacttcg ccaatgcaca tcgccaccaa gctgcgcagc
      841 acgctggacg aggtgatcga acgcgctatc tatatggtga aacgcgcccg taattacacc
      901 gatgatgttg aattttcttg cgaagatgcc gggcgtacac ccattgccga tctggcgcga
      961 gtggtcgaag cggcgattaa tgccggtgcc accaccatca acattccgga caccgtgggc
     1021 tacaccatgc cgtttgagtt cgccggaatc attagcggcc tgtatgaacg cgtgcctaac
     1081 atcgacaaag ccattatttc cgtacatacc cacgacgatt tgggcctggc ggtcggcaac
     1141 tcactggcgg cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc
     1201 gagcgtgccg gtaactgttc cctggaagaa gtcatcatgg cgattaaagt tcgtaaggat
     1261 attctcaacg tccacaccgc cattaatcac caggagatat ggcgcaccag ccagttagtt
     1321 agccagattt gtaatatgcc gatcccggca aacaaagcca ttgttggcag cggcgcattc
     1381 gcacactcct ccggtatcca ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc
     1441 atgacaccag aatctattgg tctgaaccaa atccagctga atctgacctc tcgttcgggg
     1501 cgtgcggcgg tgaaacatcg catggatgag atggggtata aagaaagtga atataattta
     1561 gacaatttgt acgacgcctt cctcaagctg gcggacaaaa aaggtcaggt gtttgattac
     1621 gatctggagg cgctggcctt catcggtaag cagcaagaag agccggagca tttccgtctg
     1681 gattacttca gcgtgcagtc tggctctaac gatatcgcca ccgccgccgt caaactggcc
     1741 tgcggcgaag aagtcaaagc agaagccgcc aacggtaacg gtccggtcga tgccgtctat
     1801 caggcgatta accgcatcac tgactataac gtcgaactgg tgaaatacag cctgaccgcc
     1861 aaaggtcacg gtaaagatgc gctgggtcag gtggatattg tcgccaacta caacggtcgc
     1921 cgcttccacg gcgtcggcct ggccaccgat attgtcgagt cctccgccaa agccatggtg
     1981 cacgtactta acaatatctg gcgtgccaca gaagtcgaaa aagagttgca acgcaaagct
     2041 caacacaacg aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat
     2101 tgccggggga tggcattggt ccggaagtga tgacccaggc gctgaaagtg ctggatgccg
     2161 tgcgcaaccg ctttgcgatg cgcatcacta ccagccatta cgatgtaggc ggcgcagcca
     2221 ttgataacca cgggcaacca ctgccgcctg cgacggttga aggttgtgag caagccgatg
     2281 ccgtgctgtt tggctcggta ggcggcccga aatgggaaca tttaccaccg gaccagcaac
     2341 cagaacgcgg cgcgctgttg cctttgcgta agcacttcaa attattcagc aacctgcgtc
     2401 cggcaaaact gtatcagggg ctggaagcat tctgtccgct gcgtgcagac attgccgcaa
     2461 acggcttcga catcctgtgc gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa
     2521 aaggccgcga aggtagcgga caatatgaaa aagcgtttga taccgaggtg tatcaccgtt
     2581 ttgagatcga gcgtattgcc cgcatcgcgt ttgaatctgc tcgtaaacgt cgccataaag
     2641 tgacgtcgat cgataaagcc aacgtgctgc aatcctctat tttatggcgt gaaatcgtca
     2701 acgagatcgc cacggaatac ccggatatcg aactggcgca tatgtacatc gacaacgcca
     2761 ccatgcagct gattaaagat ccatcacagt ttgacgttct gctgtgctcc aacctgtttg
     2821 gcgacattct gtctgacgag tgcgcaatga tcactggctc gatggggatg ttgccttccg
     2881 ccagcctgaa cgagcaaggt tttggactgt atgaaccggc gggcggctcg gcaccagata
     2941 tcgcaggtaa aaacatcgcc aacccgattg cgcaaattct gtcgctggca ctgctgctgc
     3001 gttacagcct ggatgccgat gatgcggctt ccgccattga acgcgccatt aaccgcgcat
     3061 tagaagaagg cattcgcacc ggggatttag cccgtggcgc tgccgccgtt agtaccgatg
     3121 aaatgggcga tatcattgcc cgctatgtgg cagaaggggt gtaatcatgg ctaagacgtt
     3181 atacgaaaaa ttattcgacg ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt
     3241 atatatcgac cgccacctgg tgcatgaagt gacctcaccg caggcgttcg atggtctgcg
     3301 cgcccacggt cgcccggtac gtcagccggg caaaaccttc gccaccatgg atcacaacgt
     3361 ctctacccag actaaagaca ttaatgcctg cggtgaaatg gcgcgcatcc agatgcagga
     3421 gctgatcaaa aactgcaaag aatttggcgt cgaactgtat gacctgaatc acccctatca
     3481 agggatcgtc cacgtaatgg ggccggaaca gggcgtgacc ttgccgggga tgaccattgt
     3541 ctgcggcgac tcgcataccg ccacccacgg cgcgtttggc gcactggcct ttggtatcgg
     3601 cacttccgaa gttgaacacg tactggcaac gcaaaccctg aaacagggcc gtgcgaagac
     3661 catgaaaatt gaagtccagg gcaaagccgc gccgggcatt accgcaaaag atatcgtgtt
     3721 ggcaattatc ggtaaaaccg gtagcgcagg cggcaccggg catgtggtgg agttttgcgg
     3781 cgaagcaatc cgtgatttaa gcatggaagg tcggatgacc ctgtgcaata tggcaatcga
     3841 aatgggcgcg aaagccggtc tggttgcacc tgacgaaacc acctttaact atgtcaaagg
     3901 ccgtctgcat gcgccgaaag gcaaagattt cgacgacgcc gttgcctact ggaaaaccct
     3961 gcaaaccgac gaaggcgcaa ctttcgatac cgttgtcact ttgcaggcag aagagatttc
     4021 accgcaggtc acctggggaa ctaacccagg ccaggtgatt tccgtgaacg acaatattcc
     4081 cgatccggct tcgtttgccg atccggttga acgcgcgtcg gcagaaaaag cgttggccta
     4141 tatggggctg aaaccgggta ttctgctgac cgaagtggct atcgacaaag tgtttatcgg
     4201 ttcctgcacc aactcacgta ttgaagattt acgcgcggcg gcggaaatcg ccaaagggcg
     4261 gaaagtcgcg ccaggcgtac aggcgctggt ggttcccggc tctggtccgg taaaagcgca
     4321 ggcggaagca gaaggtctgg ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc
     4381 tggctgctca atgtgtctgg cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc
     4441 ctccaccagc aaccgtaact ttgaaggccg tcaggggcgc ggcgggcgca cgcatctggt
     4501 cagcccggca atggctgccg ccgcggctgt gaccggccat ttcgccgaca ttcgcaacat
     4561 taaataagga gcacaccatg gcagagaaat ttatcaaaca cacaggcctg gtggttccgc
     4621 tggatgccgc caatgtcgat accgatgcaa tcatcccgaa acagtttttg cagaaagtga
     4681 cccgtactgg ttttggcgcg catctgttta acgactggcg ttttctggat gaaaaaggcc
     4741 aacagccaaa cccggacttc gtgctgaact tctcgcagta tcagggcgct tccattttgc
     4801 tggcacgaga aaacttcggc tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg
     4861 actacggttt taaggtggtg attgcgccga gttttgctga catcttctac ggcaatagct
     4921 ttaacaacca gctgctgccg gtgaaattaa gcgacgcaga agtggatgag ctgtttgcgc
     4981 tggtgaaagc caatccgggg attcattttg atgtagacct ggaagcgcaa gaggtgaaag
     5041 cgggtgagaa aacctatcgc tttaccattg atgccttccg ccgccactgc atgatgaacg
     5101 gtctggacag cattgggctt accttgcagc acgacgacgc cattgcggct tatgaagcga
     5161 agcaacccgc gtttatgcgt tagtttgtac accgtccgat actcaatcgc caaaaagaaa
     5221 aaagccagca gataatcact gctggcgtgg cgattaacac caatactcag tcacacatga
     5281 tgtccgcaaa tcagtagtca gaatgactcc cctcccactg catcagttct tcaagcatgg
     5341 ttaatcgttg tgggacgctt aaccaacgca accagcacaa cttcgctgtc gcggtaaaat
     5401 agtgctgata aaactgacga atggggcacc tccttgcttc atcgttagga attattgact
     5461 taatataggg aaaataaaat tgctgtcttt tgcgcaggag ttcccctttt atgccatctg
     5521 ctcgtctgca acaacagttc atccgcctgt ggcaatgctg cgagggtaaa tcgcaggaca
     5581 caacgctcaa cgaactggca gcgttattga gctgctcgcg tcgtcatatg cgtaccctgc
     5641 tcaacaccat gcaggatcgc ggctggctga cgtgggaagc ggaagtcggg cgtggtaaac
     5701 gctcgcgtct gacattcctc tataccgggc tggcgcttca gcaacagcgg gcggaagacc
     5761 tgctggagca ggaccgtatc gaccaactag tgcagttagt tggcgacaaa gcgactgtga
     5821 ggcaaatgct ggtttctcat ctgggccgca gcttccgcca ggggcggcac atcctgcgcg
     5881 tgctctacta tcgtccgttg cgtaatctgc tacctggcag cgcattgcgc cgttccgaaa
     5941 cccatatcgc ccggcaaatc ttcagttcgc taacgcgcat aaatgaggaa aatggggaac
     6001 tggaagcaga catcgcccac cactggcagc aaatatcacc gcttcactgg cgtttctttt
     6061 tgcgtccagg agtccatttt catcatggtc gtgaactgga aatggacgac gtgatcgcct
     6121 ctttaaaacg aatcaatacg ctgccgctct attcgcatat tgctgacatt gtgtcgccga
     6181 cgccctggac gctggatatc cacctcacgc agccggatcg ctggttgccg ttactgctgg
     6241 gacaagttcc ggcgatgatc ctgccgcgcg aatgggaaac cctcagtaac tttgccagcc
     6301 atcccatcgg caccggtccg tatgcggtga ttcgcaacag caccaatcaa ctgaaaattc
     6361 aggcattcga tgacttcttc ggttaccggg cattaatcga tgaagttaac gtctgggttc
     6421 tgccggaaat tgccgacgag ccagccggag ggctgatgct aaaaggtcca cagggcgagg
     6481 aaaaagagat tgaaagccgc ctggaggaag gttgctacta tttactgttc gatagccgca
     6541 cccatcgcgg ggcgaatcag caagtcaggg actgggtaag ctatgtgctt tctccaacta
     6601 atctggtcta tttcgctgag gaacagtacc agcaactgtg gttcccggct tatggactgc
     6661 tcccccgttg gcatcatgcc cgcaccataa cgagcgaaaa accggctggt ctggaaagcc
     6721 tcaccctgac cttttatcag gatcacagtg agcatcgggt gattgccggg atcatgcagc
     6781 agattctggc aagccaccag gtcacactgg aaatcaaaga gatcagctac gatcagtggc
     6841 atgaaggaga gatcgagagc gatatctggc ttaacagcgc caactttacg ctgccgctgg
     6901 atttttcgct gttcgcgcac ctgtgcgagg taccgctgct ccaacactgt attccaatcg
     6961 actggcaagt cgacgccgcc cgctggcgca atggcgaaat gaacctggcg aactggtgcc
     7021 aacaactggt cgccagcaaa gcaatggtgc cacttatcca ccactggctg atcattcagg
     7081 gacaacgcag tatgcgcggc ctgcgcatga acaccctcgg ctggtttgat tttaaatcag
     7141 cgtggtttgc gccgccggat ccagagtagc ttgcgccttt tggcggtcat gagcgcattt
     7201 tttatattca gacggaatga taaaatacat tacttttata ttttctctgt tttatgcatc
     7261 gtctgattag cgtcgcaaat aagattaaag aaggggcatt ccagcccctc tttcatattc
     7321 tgagttaatc aataacggcg cagtctagcc ttgttctggc cggaaaggtc agctttttca
     7381 ggttaacctc gcgcaggtca gtcataccaa cggctattgt tcgtttcagc attgctcagg
     7441 tcaacctcga gacagtcaat accacaaatg acaaccccgt taaagtaagc attactcaga
     7501 tcaatccccc cggtcctggt gctctttttc agctgcaact ccagagtaaa tatcgcctca
     7561 tcgccaataa aaaaactgac gcataacgta ccttctggcg agagtgaggc cttcgcggcc
     7621 atattttccg gatcttttgc gtattctttg agttcattaa attttatcag ccagtgagtt
     7681 aacttatcac gcgccttccc gtcgacagcc ccggtaaagt attttgagta gtcatcacta
     7741 ttctggaaat aaaatgcatc cttcagaatt tgccttgccg cagtctggga atgtttatct
     7801 gactggaaga gattgcgtaa cgtctcccat agctcttcaa agatattcat attctcggtc
     7861 tttttgacca agttagccat cctgataatc tgagaattag taaggcctgg atctttagtg
     7921 gtattactag cagggatact aatctgttct gggtttctta ctttcatgct gatagctcca
     7981 ctctgaataa aaaactcaga ataagcgtcc tgaataagaa tactgtcaaa aatcgctcat
     8041 tcacgcaata aacggttacg ttacctattt tgactgtagc gaagggattc tttcggatca
     8101 acgctctcca gctatgcaaa tacgctcttg atagctgaac gaatattggc ttttttaata
     8161 cgccttctct atggattgct ggttgataac aaaatcacta cactaacgcc gttctcaacg
     8221 gggtgccacg cggacgcgtg cgctgagaaa atacccgtcg aacctgatcc ggataacgcc
     8281 ggcgaaggga tttgaggctc cttctcaagt cctttgccac tcttttttga ggtgcaaagt
     8341 gttaaaaaaa tgtcttcccc tgctgttgct gtgcacagcg cccgttttcg ctaaacccgt
     8401 tctgactgtt tatacctacg attccttcgc cgccgactgg gggcctggtc cggtggttaa
     8461 aaaagccttt gaagccgact gtaattgcga actgaaactg gtggcgctgg aagatggcgt
     8521 ttcgcttctc aaccgtctac ggatggaagg caaaaacagt aaagccgatg tggtgctggg
     8581 gctggataac aatctgttag acgccgccag taaaaccgga ctgtttgcca aaagcggtgt
     8641 ggcagcggat gccgttaacg ttcccggcgg ctggaataat gacattttcg taccgtttga
     8701 ttatggctac ttcgccttcg tttatgacaa gaacaaactg aaaaacccgc cacaaagcct
     8761 gaaagaactg gttgagagcg atcaaaactg gcgggtgatt tatcaggatc cgcgcaccag
     8821 tacaccgggg ctgggtctgt tgctatggat gcaaaaagtc tatggcgatg acgccccaca
     8881 agcctggcag aaactggcga agaaaacggt cacggtcacc aaaggctgga gcgaagccta
     8941 cggcctgttt ttaaaaggtg aaagcgatct ggtactgagt tacaccacct ctccggctta
     9001 tcacattctc gaagagaaga aagataacta cgccgccgcg aacttcagcg aaggtcacta
     9061 tctgcaagtg gaagtcgccg ctcgcaccgc tgccagcaag cagccggagc tggcgcaaaa
     9121 attcctccag tttatggttt ctccggcttt ccagaatgcg atcccaaccg gcaactggat
     9181 gtatccggtg gcaaacgtca cgctgcctgc cggttttgaa aaattgacca aacccgcaac
     9241 cacgctggag ttcacgccag ccgaagtggc ggcacaacgt caggcatgga ttagcgaatg
     9301 gcaacgcgcc gtcagccgtt aattcccggc tggttaattc caggtgtaag cgccgccacg
     9361 ctggtggtag cggttgcgct ggcggcgttt ctcgccctgt ggtggaacgc gccgcagggt
     9421 gactggtcgg cagtctggca ggacagctac ctgtggcatg tggtgcgctt ctccttctgg
     9481 caggcgtttc tctcggcgct actctctgtc gtacccgcga tattcctcgc ccgcgcgctc
     9541 tatcgcaggc gctttccggg tcggctggcg ctgttgcgtc tgtgcgcaat gaccttgatc
     9601 ctcccggtgc tggttgctgt tttcggcatt cttagcgtct atggtcgcca gggctggctg
     9661 gcatcgctct gccaatcgct cggtctggag tggacctttt cgccctacgg cctgcaaggc
     9721 attttgctgg cccatgtgtt ttttaatctg ccgatggcga gccgcttatt actccaggca
     9781 ctggaaaaca ttcccggcga acaacgtcag cttgccgccc agcttgggat gcgcggctgg
     9841 cattttttcc gcttcgtcga atggccgtgg ttacggcgac aaatcccgcc ggttaccgcg
     9901 cttatcttta tgctctgttt cgccagcttc gccaccgtgt tatcgctggg cggcggtccg
     9961 caggcaacca ctatcgagct ggcaatctat caggcgctga gttacgacta cgatcctgcc
    10021 cgcgcggcga tgctggcgct gatccagatg gtgtgttgcc ttgggctggt gctgttgagt
    10081 cagcgactga gtaaggccat tgcgcccggc accacgctgc tgcaaggctg gcgcgacccg
    10141 gacgatcgtc tgcatagccg catttgcgac acgatgttaa ttgtgctggc gctgctgctg
    10201 ttgctgccac cgttgctggc ggtgatcgtc gatggggtaa atcgccagtt gccggaagtg
    10261 ctggcacaac cggtgctgtg gcaggcgctg tggacctcgt tgcgtattgc gctggcggca
    10321 ggtgtattgt gcgtagtgct gaccatgatg ctgctatgga gcagtcgcga actgcgggcg
    10381 cggcaaaaaa tgctggcggg ccaggcgctg gagatgagcg gcatgttgat cctcgctatg
    10441 ccggggattg tgctggcaac gggcttcttt ttactgctca acaacactat cggcctgcca
    10501 caatctgctg acggcattgt gattttcacc aatgcgttaa tggcgatccc ctacgccctg
    10561 aaagtgctgg aaaacccgat gcgcgatatc accgcccgct acagcatgtt atgtcagtcg
    10621 ctggggattg aaggctggtc acgcttaaaa gtagtggagc tgcgcgccct gaaacgcccg
    10681 ctggcgcagg cgctggcctt tgcctgcgtg ctgtcgattg gtgattttgg cgtggtggcg
    10741 ttgttcggta acgatgattt ccgcaccctg ccgttttatc tctaccagca aattggctcc
    10801 tatcgcagcc aggacggcgc ggtcaccgcg ttaattctgc tgctgctctg ttttctgctg
    10861 tttagcgtga ttgaaaaaat accggggcga aatgttaaaa ctgactgata tcacctggct
    10921 ttatcaccat ttgccgatgc gttttagctt aacggtagaa cgcggcgagc aggtggcgat
    10981 cctcgggcca agcggcgcgg gtaaaagtac cctgctgaac ttgatcgccg gttttctgac
    11041 accagccagc ggtgcgctga ctatcgacgg cgttgatcac accacgacgc cgccgtcacg
    11101 ccgtccggtg tcgatgctgt ttcaggagaa caacctgttc agccacctga cggtcgcgca
    11161 gaacatcggg ctggggctta atccaggatt gaagctgaac gcggtacagc aggagaaaat
    11221 gcacgcgatt gcccgtcaga tggggattga taatttaatg gcgcggttac cgggcgagct
    11281 ttccggcggt cagcgacagc gcgtggcgtt agcgcgttgt ctggtacgcg aacagccgat
    11341 tttattgctc gatgaaccgt tctctgcgct cgatccggcg ctacgtcagg agatgttgac
    11401 gctggtgagc actagctgcc agcagcaaaa aatgacgcta ttgatggtgt cgcacagtgt
    11461 ggaagatgcg gcgcggattg ccacgcgctc ggtagtagtc gccgacgggc gaatcgcctg
    11521 gcagggtatg accaatgagt tgttgagcgg taaggcgagt gcttcggcgc tgttgggaat
    11581 tacaggttaa tatgcactgt cggatgcggc gtgaacgcct tatccgacct acgggtgcgt
    11641 tttgtccgca tcaagcgccc cacacaagac tatgccctaa actccaacca ctttacgcaa
    11701 aatatcgata tacaccggca ttaacggatg gcgaactaac accaccagcg ccaccacgcc
    11761 gatggcagaa atcaacggcg tcagccacaa caaacgaccg cgggacaaat aatgactcaa
    11821 acgatcggtt gctttgccgc tgcgccataa ccgccagcac agccagccac caacccacaa
    11881 aaataccgcc gtcgccagca gcaaccattt aaactcaccg ctctgcattc cggctggaat
    11941 atcgatcgcc gcacccgcca gaatccccgg caggaagtaa aacggtggcc acagcaggca
    12001 gccgataata ttcggcgtga taaattttgc caccgggaga tccagcattc ccgccaccat
    12061 tggcaccagc ggacgcgtcg ggccaacaaa acgaccgacc agaatggtga acatgctgtg
    12121 ttgatgcaac gcatgttcag ttttatcgag taatgctttg ttcttcttca gaaatgacca
    12181 acgatgcaat ggctttttaa aacgccagcc cagccagaag gagatccagt cgcccagcag
    12241 acaaccgacg atccctgcca gccaggcgtg ccagaaactt aactcgccgc tgccaatcag
    12301 cgctcccagc cccgccatca gcaccgtacc gggtagaatc aaaccgacca gcgccagcga
    12361 ctccagaaag gccaccaaca ccaccgccat caatgaatac acggtggatt gggtaataaa
    12421 gtgttccagc aatgcttgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa
    12481 ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga
    12541 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
    12601 tgcatttttt aaatactcgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
    12661 cggtggcgat aggcatccgg gtagtgctca aaagcagctt cgcctggctg atgcgttggt
    12721 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
    12781 acggcgacag gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
    12841 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact
    12901 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct
    12961 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
    13021 gcggctggtg cgcttcatcc gggcgaaaga aacccgtatt ggcaaatatt gacggccagt
    13081 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
    13141 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
    13201 ccggtcggca gacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
    13261 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccat
    13321 tggcctcaat cggcgttaaa cccgccacca gatgggcgtt aaacgagtat cccggcagca
    13381 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca
    13441 attgtccata ttgcatcaga tattgccgtc actgcgtctt ttactggctc ttctcgctaa
    13501 cccaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga
    13561 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gataatttgc
    13621 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct
    13681 gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg atggagtgaa
    13741 acgatggcga ttgcaattgg cctcgatttt ggcagtgatt ctgtgcgagc tttggcggtg
    13801 gactgcacca ccggtgaaga aatcgccacc agcgtagagt ggtatccccg ttggcaaaaa
    13861 gggcaatttt gtgatgcccc gaataaccag ttccgtcatc atccgcgtga ctacattgag
    13921 tcaatggaag cggcactgaa aaccgtgctt gcagaactta gcgtcgaaca gcgcgcagct
    13981 gtggtcggga ttggcgttga cagtaccggc tcgacgcccg caccgattga tgccgacggt
    14041 aacgtgctgg cgctgcgccc ggagtttgcc gaaaacccga acgcgatgtt cgtattgtgg
    14101 aaagaccaca ctgcggttga agaagcggaa gagattaccc gtttgtgcca cgcgccgggc
    14161 aatgttgact actcccgcta tattggcggt atttattcca gcgaatggtt ctgggcaaaa
    14221 atcctgcatg tgactcgcca ggacagcgcc gtggcgcaat ctgccgcatc gtggattgag
    14281 ctgtgcgact gggtgccagc tctgctttcc ggtaccacgc gcccgcagga tattcgtcgc
    14341 ggacgttgca gcgccgggca taagtcgcta tggcacgaaa gctggggcgg cctgccgcca
    14401 gccagtttct ttgatgagct ggatccgatc ctcaatcgcc atttgccttc cccgctgttc
    14461 actgatacct ggactgccga tattccggtg ggcaccttat gcccggaatg ggcgcagcgt
    14521 ctcggcctgc ctgaaagcgt ggtgatttcc ggcggcgcgt ttgactgcca tatgggcgca
    14581 gttggtgcag gcgcacagcc taacgcactg gtaaaagtta tcggtacttc cacctgcgac
    14641 attctgattg ccgacaaaca gagcgttggc gagcgggcag tgaaaggtat ttgcggtcag
    14701 gttgatggca gcgtggtgcc tggatttatc ggtctggaag caggccaatc ggcgtttggt
    14761 gatatctacg cctggttcgg tcgcgtactc ggctggccgc tggaacagct tgccgcccag
    14821 catccggaac taaaagagca aatcgacgcc agccagaaac aactgcttcc ggcgctgacc
    14881 gaagcatggg ccaaaaatcc gtctctggat cacctgccgg tggtgctcga ctggtttaac
    14941 ggccgccgca caccgaacgc taaccaacgc ctgaaagggg tgattaccga tcttaacctc
    15001 gctaccgacg ctccgctgct gttcggcggt ttgattgctg ccaccgcctt tggcgcacgc
    15061 gcaatcatgg agtgctttac cgatcagggg atcgccgtca ataacgtgat ggcgctgggc
    15121 ggcatcgcgc ggaaaaacca ggtcattatg caggcctgct gcgacgtgct gaatcgcccg
    15181 ctgcaaattg ttgcctctga ccaatgctgt gcgctcggtg cggcgatttt tgctgccgtc
    15241 gccgcgaaag tgcacgcaga catcccatca gcccagcaaa aaatggccag tgcggtagag
    15301 aaaaccctgc aaccgcgcag cgaacaggca caacgctttg aacagcttta tcgccgctat
    15361 cagcaatggg cgatgagcgc cgaacaacac tatcttccaa cttccgcccc ggcacaggct
    15421 gcccaggccg ttccgactct ataaggacac gataatgacg atttttgata attatgaagt
    15481 gtggtttgta attggcagcc agcatctgta tggcccggaa accctgcgtc aggtcaccca
    15541 acatgccgag cacgttgtga aagcgctgaa tacggaagcg aaactgccct gcaaactggt
    15601 actgaaaccg ctgggcacca cgccggatga aatcaccgct atttgccgtg atgctaatta
    15661 cgacgatcgt tgcgctggtc tggtggtgtg gctacacacc ttctccccgg ccaaaatgtg
    15721 gatcaacggc ctgaccatgc tcaacaaacc gttgctgcaa ttccacaccc agttcaacgc
    15781 ggcgctgccg tgggacagca tcgatatgga ctttatgaac ctgaaccaga ccgcgcacgg
    15841 cggtcgcgag ttcggcttca ttggcgcgcg tatgcgtcag caacatgccg tggttaccgg
    15901 tcactggcag gataaacaag cccatgagcg tatcggctcc tggatgcgtc aggcggtatc
    15961 taaacaggat acccgtcatc tgaaagtctg ccgatttggc gataacatgc gtgaagtggc
    16021 ggtcaccgat ggcgataaag ttgccgcaca gatcaagttc ggtttctccg tcaatacctg
    16081 ggcggttggc gatctggtgc aggtggtgaa ctccatcagc gacggcgatg ttaacgcgct
    16141 ggtcgatgag tacgaaagct gctacaccat gacgcctgcc acacaaatcc acggcgaaaa
    16201 acgacagaac gtgctggaag cggcgcgtat tgagctgggg atgaaacgtt tcctggaaca
    16261 aggtggcttc cacgcgttca ccaccacctt tgaagatttg cacggtctga aacagcttcc
    16321 tggtctggcc gtacagcgtc tgatgcagca gggttacggc tttgcgggcg aaggcgactg
    16381 gaaaactgcc gccctgcttc gcatcatgaa ggtgatgtca accggtctgc agggcggcac
    16441 ctcctttatg gaggactaca cctatcactt cgagaaaggc aatgacctgg tgctcggctc
    16501 ccatatgctg gaagtctgcc cgtcgatcgc cgcagaagag aaaccgatcc tcgacgttca
    16561 gcatctcggt attggtggta aggacgatcc tgcccgcctg atcttcaata cccaaaccgg
    16621 tccagcgatt gtcgccagct tgattgatct cggcgatcgt taccgtctac tggttaactg
    16681 catcgacacg gtgaaaacac cgcactccct gccgaaactg ccggtggcga atgcgctgtg
    16741 gaaagcgcaa ccggatctgc caactgcttc cgaagcgtgg atcctcgctg gtggcgcgca
    16801 ccataccgtc ttcagccatg cgctgaacct caacgatatg cgccagttcg ccgagatgca
    16861 cgacattgaa atcacggtga ttgataacga cacccgcctg ccagcgttta aagacgcgct
    16921 gcgctggaac gaagtgtatt acgggtttcg ccgctaagta gccgcatccg gtatgtaacg
    16981 cctgatgcga cgctggcgcg tcttatcagg cctacacgct gcaattttgt aggccggata
    17041 agcaacgcgc atccggcatt cagcgcctga tgcgacgctg acgcgtctta tctggcctac
    17101 acgctgcgat tttgtaggcc ggataagcaa cgcgcatccg gcacgaagga gtcaacatgt
    17161 tagaagatct caaacgtcag gtattagaag ccaacctggc gctgccaaaa cacaacctgg
    17221 tcacgctcac atggggcaac gtcagcgccg ttgatcgcga gcgcggcgtc tttgtgatca
    17281 aaccttccgg cgtcgattac agcatcatga ccgctgacga tatggtcgtg gtcagcatcg
    17341 aaaccggtga agtggttgaa ggtgcgaaaa agccctcctc cgatacgcca actcaccgac
    17401 tactctatca ggcattcccg tccattggcg gcattgtgca cacgcactcg cgccacgcca
    17461 ccatctgggc gcaggcgggt cagtcgattc cagcaaccgg caccacccac gccgactatt
    17521 tctacggcac cattccctgc acccgcaaaa tgaccgacgc agaaatcaac ggcgaatatg
    17581 agtgggaaac cggtaacgtc atcgtagaaa ccttcgaaaa acagggtatc gatgcagcgc
    17641 aaatgcccgg cgttctggtc cattctcacg gcccatttgc atggggcaaa aatgccgaag
    17701 atgcggtgca taacgccatc gtgctggaag aggtcgctta tatggggata ttctgccgtc
    17761 agttagcgcc gcagttaccg gatatgcagc aaacgctgct ggataaacac tatctgcgta
    17821 agcatggcgc gaaggcatat tacgggcagt aatgactgta taaaaccaca gccaatcaaa
    17881 tgaaaccagg ctataatcaa gcctggtttt ttgatggatt ttcagcgtgg cgcaggcagg
    17941 ttttatctta acccgacact ggcgggatac cccgcaagga acagaggtct ccttctggct
    18001 ggcgacggac aacgggccgt tgcaggttac gcttgcaccg caagagtccg tggcgtttat
    18061 tcccgccgat caggttcccc gcgctcagca tattttgcgg ggtgaacaag gctttcgcct
    18121 gacgccgctg gcgttaaagg attttcaccg ccagccggtg tatggccttt actgtcgcgc
    18181 ccatcgccag ttgatgaatt acgaaaagcg cctgcgtgaa ggtggcgtta ccgtctacga
    18241 ggccgatgtg cgcccgccag aacgctatct gatggagcgg tttatcacct caccggtgtg
    18301 ggtcgagggt gatatgcaca atggcgctat cgttaatgcc cgtctgaaac cgcatcccga
    18361 ctatcgtccg ccgctcaagt gggtttctat tgatattgaa accacccgcc acggagagct
    18421 gtactgcatc ggcctggagg gctgcgggca gcgcatcgtt tatatgctgg gaccagagaa
    18481 tggcgacgcc tccgcgctcg attttgaact ggagtacgtc gccagccgcc cgcagctact
    18541 ggaaaaactc aacgcctggt ttgcaaacta cgatcctgat gtgatcatcg gttggaacgt
    18601 ggtgcagttt gatctgcgaa tgctgcaaaa acatgccgag cgttatcgta ttccgctgcg
    18661 gctggggcgc gataacagtg agctggagtg gcgcgagcac ggctttaaaa acggcgtctt
    18721 ttttgcccag gctaaaggtc ggctaattat cgacggtatc gaggcgctga aatccgcgtt
    18781 ctggaatttc tcttcattct cgctggaaac tgtcgctcag gagctattag gcgaaggaaa
    18841 atctatcgat aacccgtggg atcgaatgga cgaaattgac cgccgtttcg ccgaagataa
    18901 acctgcgctg gcaacttata acctgaaaga ttgcgagctg gtgacgcaga tcttccataa
    18961 aactgaaatc atgccgtttt tgctcgaacg agcaacggtg aacggcctgc cggtggatcg
    19021 acacggcggg tcggtggcag cgtttggtca tctctatttt ccccgtatgc atcgcgctgg
    19081 ttatgtcgcg cctaatctcg gcgaagtgcc gccgcacgcc agccctggcg gctacgtgat
    19141 ggattcgcga cctgggcttt atgattcggt gctggtgctg gactacaaaa gcctgtaccc
    19201 gtcgatcatc cgcacctttc tgattgatcc cgtcgggctg gtggaaggca tggcgcagcc
    19261 tgatccagag cacagcaccg aaggctttct cgatgcctgg ttctcgcgag aaaaacattg
    19321 cctgccggag attgtgacta acatctggca cggacgcgat gaagccaaac gccagggtaa
    19381 caaaccgctg tcgcaggcgc tgaaaatcat catgaatgcc ttttatggcg tgctcggcac
    19441 caccgcctgc cgcttcttcg atccgcggct ggtatcgtcg atcaccatgc gtggtcatca
    19501 gatcatgcgg caaaccaaag cgttgattga agcgcagggc tacgacgtga tctacggcga
    19561 taccgactca acgtttgtct ggctgaaagg cgcacattcg gaagaagaag cgacgaaaat
    19621 cggtcgtgca ctggtgcagc acgttaacgt ctggtgggca gaaacgctgc aaaaacaaca
    19681 gctgaccagc gcactggaac tggagtatga aacccatttc tgccgttttc tgatgccaac
    19741 cattcgcgga gccgataccg gcagtaaaaa gcgttatgcc gggctgattc aggagggcga
    19801 caaacagcgg atggtgttta aagggctgga aaccgtgcgt acggactgga cgccgctggc
    19861 ccagcagttt cagcaggagc tttacctgcg tatcttccgc aacgagccat atcaggagta
    19921 cttccgcgaa accatcgaca aactgatggc gggtgaactg gatgcgcggc tggtttaccg
    19981 taaacgcctt cgccgtccgc tgagcgaata tcagcgtaat gttccgcctc atgtacgcgc
    20041 cgctcgtctt gccgatgaag aaaaccaaaa gcgcggtcgc cccttgcaat atcagaaccg
    20101 cggcaccatt aagtacgtat ggaccaccaa cggcccggag ccgctggact accaacgttc
    20161 accactggat tacgaacact atctgacccg ccagctacaa cccgtggcgg agggaatact
    20221 cccttttatt gaggataatt ttgctacact tatgaccggg caacttgggc tattttgagc
    20281 aaaaaaagag ttcgccagat accattttga tgcgtgacga atgctttgcc atccagtacc
    20341 atagcgccct ttccattcct ggacctgaat aacaccacta cctcataagc acggtagcgg
    20401 gtggttattg cctgcaatta aagatataga gccgaacaca tatgcctttt acacttggtc
    20461 aacgctggat cagcgataca gaaagcgaat tgggacttgg aaccgttgtc gcggtggatg
    20521 cgcgaactgt cactttactt ttcccatcta ctggtgaaaa ccgtctgtac gcacgcagtg
    20581 attcccccgt gacccgcgtg atgttcaacc ctggtgatac cattaccagc catgacggct
    20641 ggcagatgca agtcgaagaa gtaaaagaag aaaatggctt gctgacctat atcggtactc
    20701 gcctggatac tgaagagtcc ggcgtagccc tgcgtgaagt tttccttgat agcaaactgg
    20761 tgttcagcaa accgcaggac cgcctgtttg ccgggcagat tgaccgtatg gaccgctttg
    20821 cgctgcgtta tcgcgcgcgt aagtattcca gcgaacagtt ccgtatgccg tacagcggcc
    20881 tgcgcggtca gcgtaccagc ctgatcccgc accagctcaa catcgctcat gatgttggcc
    20941 gccgccacgc gccgcgcgtc ctgctggctg acgaagtggg tttagggaaa accattgaag
    21001 ccgggatgat ccttcatcag caactgctct ctggcgctgc tgaacgtgtg ctgattatcg
    21061 tcccggaaac cttacagcat cagtggctgg tagaaatgct gcgccgtttc aacctgcgct
    21121 ttgcgctgtt tgatgatgag cgttatgccg aagctcagca cgatgcttac aacccgttcg
    21181 acaccgaaca gctggtgatt tgctcgctgg attttgcccg ccgtagcaaa cagcgtctgg
    21241 aacatctctg tgaagccgag tgggacctgc tggtggtcga tgaagcgcat cacctggtgt
    21301 ggagcgaaga cgcgccgagc cgcgaatatc aggctattga acaactggca gagcacgtgc
    21361 cgggcgttct gctgctgacc gcaaccccgg aacagctggg aatggaaagc cacttcgccc
    21421 gtctgcgtct gctggacccg aaccgtttcc acgatttcgc gcaattcgtt gaagagcaga
    21481 aaaattatcg tccagtagcg gacgccgttg ccatgctgct ggcaggtaac aaactgagca
    21541 atgacgaact gaacatgctt ggcgagatga tcggcgagca ggatatcgag ccgctgttgc
    21601 aagcagcaaa cagcgacagc gaagatgccc agagcgcccg tcaggagctg gtttcgatgc
    21661 tgatggatcg ccacggcacc agccgcgtgc tgttccgtaa cacccgtaac ggtgtgaaag
    21721 gcttcccgaa acgcgagctg cacaccatta agctgccgct accgacgcag tatcagacgg
    21781 ctattaaagt ctccggcatt atgggcgcac gtaaaagtgc ggaagaccgc gcccgcgata
    21841 tgctctaccc ggagcgtatt tatcaggaat ttgaaggtga taacgccacc tggtggaact
    21901 tcgatccgcg cgttgagtgg ctgatgggct acctgaccag ccatcgctct cagaaagtgc
    21961 tggtgatctg cgctaaagct gccactgcgc tgcaactgga gcaagtactg cgcgaacgtg
    22021 aaggtattcg cgctgcggtg ttccacgaag gtatgtcgat tatcgaacgt gaccgcgctg
    22081 ccgcctggtt tgccgaagaa gacaccggcg cacaggtact gctgtgctca gaaatcggtt
    22141 ctgaaggacg taacttccag ttcgccagcc acatggtgat gtttgacctg ccattcaacc
    22201 cggatctact ggagcagcgt attggtcgtc tggatcgtat cggccaagcg cacgatattc
    22261 agatccatgt gccttatctg gagaaaaccg ctcagtcggt gctggtgcgc tggtatcacg
    22321 aaggtctgga tgcatttgag cacacctgcc cgaccggacg cactatttac gatagcgtat
    22381 acaacgatct gattaactat ctggcttcac cggatcaaac cgaaggcttt gacgatctga
    22441 tcaaaaactg ccgcgagcaa catgaagcgc tgaaagcaca gctggaacag ggtcgtgacc
    22501 gcctgctgga aatccactcc aacggtggcg aaaaagccca ggcactggca gaaagcattg
    22561 aagagcagga tgacgatacc aacctgatcg ccttcgccat gaacctgttc gatattatcg
    22621 gtatcaatca ggacgatcgc ggcgacaaca tgatcgtgct gacgccgtcc gatcatatgc
    22681 tggtgccgga cttccctggc ctgtcggaag atggcatcac catcaccttt gatcgtgaag
    22741 tggcgctggc gcgtgaagat gcgcagttta ttacctggga acatccgctg atccgcaacg
    22801 gtctggatct gattctttct ggcgataccg gtagcagcac gatttcactg ttaaaaaaca
    22861 aagcgttgcc ggtaggtacg ctgttggtgg aactgattta tgtggttgaa gcccaggctc
    22921 cgaagcagtt gcagctcaac cgcttcctgc caccgacgcc ggtacgtatg ctgctggata
    22981 aaaacggcaa caacctggcg gcgcaggtag agtttgaaac ctttaaccgc cagcttaacg
    23041 cggttaaccg tcacaccggc agcaaactgg ttaacgccgt gcagcaggat gttcacgcta
    23101 tccttcaact gggtgaagcg cagatcgaga aatctgcccg tgcattgatt gatgcagcac
    23161 gtaacgaagc cgacgaaaaa ctgtctgccg agctgtctcg tctggaagct ctgcgtgcag
    23221 tgaacccgaa cattcgtgac gacgaactga ccgccattga gagcaaccgt cagcaggtaa
    23281 tggaaagcct ggatcaggca ggttggcgtc tggatgccct gcgtttgatc gttgtaacgc
    23341 atcagtaacg gagccgaaaa tggggatgga aaactacaat ccaccgcagg aaccctggtt
    23401 ggttatcctg tatcaggatg accatattat ggtggtcaac aagccgagcg gtttgttgtc
    23461 tgtgccgggt cgtctggaag agcacaaaga cagcgtgatg acgcgcattc agcgagatta
    23521 tccgcaggca gaatcggtgc atcgtctgga tatggctacc agcggcgtga ttgtagtggc
    23581 gctgaccaaa gccgcggagc gggagttaaa acgccagttc cgcgagcgcg tgccgaaaaa
    23641 gcagtatgtg acccgcgtct gggggcatcc atcccccgca gaaggtctgg tggatctgcc
    23701 gctgatttgc gactggccaa accgcccgaa acagaaagtc tgttacgaaa cgggtaaacc
    23761 tgcgcagacg gaatatgaag tggtggagta tgcggcggat aacacggcaa gagtggtgtt
    23821 aaaaccgatt accgggcgtt cgcatcaatt gcgtgtgcat atgctggcgc tggggcatcc
    23881 gattctcggc gatcgttttt atgcatcacc agaagcgaga gcgatggcac cgcgtttgtt
    23941 gctacatgca gagatgttga cgattaccca tccggcgtat ggcaatagta tgacgtttaa
    24001 agcgccagcg gatttttaac ctttcctgta cgtttatacc ctcatcctaa gggctgcctc
    24061 ttgtacataa atccccgagt ccggggattt tttgtttctg ccaaactgct ttcagttgtt
    24121 gcgtgtggct acgctttcca ttcggtaatt cccttcctgc tccacagcga atttttgaac
    24181 tttttcttca cccgttgacc gtctcttata tttcatttta tcgggtctgt tatggtcctt
    24241 tctgattgtt attcatgggc taattagcag tctggttggc gatgcccatc tcggtgatcc
    24301 gcgtcgtaca cgacgccttg cgtcactagc ctcttctcta gctcatcatg caggtctttc
    24361 taattgtgaa atcatcccac gctactgcta aggttgaagg ggcgttccag ccccttcaac
    24421 cttagcttat tctggctcat tcaataattg cgttgtcagt aatttgttca gcagtacttt
    24481 gtttagtagc aacttcttca gtatcaattt ttttgggggt gattttatta ataataatta
    24541 cctcatcata taggtgggta gcaccgtcca gggtagcacc gtccagggta gcaccgtcca
    24601 ggttagcatt ccacccaggt tccaagtaaa ggactttatt gtgacctttc attgttttga
    24661 gaccaaggta acaatccctc atgtcaacgc cgggaaggta agcctctcta agatcaacgc
    24721 ccaagttacc tgggccttta tgcagctgca acgtctgagt acatatctcc ttattttcaa
    24781 taaaaaaagt gacacttaac tcgatatcac cgaaggtctg tgcgacccag ctcatattgc
    24841 cctttgcgac catctcatcc ggattttttg cgcatgtttt gagttcattg aataattcca
    24901 gactatggat aaacttttct ttctcattct cgttgacagc cccagtaaag tattttgagg
    24961 cgttgatgtt gccagccttt ttattcacga aattgaacag aagtttataa gcctcagtct
    25021 gtttatctga ccagaacaga tgacgtaacg tctcccacaa gcgttgaaag atattcatct
    25081 tcatgtccat cttgtccccg ctcaccatct tataaatatc gttattgctc atatttaaaa
    25141 gtgtagccgg catgcctgga actgatactt tcatggtaat agcctcgcat tgagttaaaa
    25201 actcatgatg cgcgctctga ataaatatac tttcaaaaat cgctcattca gatatgtgtt
    25261 ttttattttt aagacgtaaa aatataattc cgctcgccgt aaagctctca accttaagca
    25321 gggtcaacgc gataaaacca acagaaaccc tcccgtaaac cgtatattga gcctgtccac
    25381 cattctgagt aaacgccttt tctcaaagaa gtgaccgcca gggcggccac cttttatcgt
    25441 gggcagttac tcagcatcat ttacagggtc atttaaaccc tttctgctgc ttaataagct
    25501 cataggcctg ctgaatttcc tgcgctttct gcttcgccat ctccatcatc tcaggcggca
    25561 aacctttcgc caccagctta tcgggatggt gttcactcat cagcttacgg taggcacgtt
    25621 tgatggtggt cgcatcatcc gtcggcttca cgcccagcac attacaggca tcttccagcg
    25681 ttgggccacg ctgcgcttgc tgccagttac caccgccaga ttgctgctga taaccgccgc
    25741 caaactgtgc accgccctgc atcatgcgca aaaactggtc aaactgggcg cgggagatcc
    25801 ctaattcttc agcaatgaca tacagcaccg cccgttcatt cgggtgcagt gaaccatcag
    25861 caaacgccgc ctgaatctgg atctccagaa acatacgaat taagtcaaaa cgaccaaagc
    25921 agacactgcg aaactggcgc atcttttcgc gcagcgggta attgtctgat tttcccaccc
    25981 ggaacgcatt ttgcgccgca gtacgggaag cgccatgaag attcattcgg tccatcaact
    26041 ggctggcgat atgaatatca gcctccgtga cgcgaccttt ggatttggtt aaatgcccca
    26101 tcacttcaaa agtggtggca aaaaacagcg cctgacgctc acgctggttg gcgaaccacg
    26161 ccattttacg gctacgggct ttatcaaaca tatggccaat taacaggcct aacactacgc
    26221 cccaaaagcc gccgcccatc agtaaggcca cggccacgcc aatgattttt ccccaatact
    26281 gcatatattc cccaaatcga cacacggata tcagggctat ctcccacaat ataaaggtgc
    26341 ttttaccgtt ttccggctgc ggtcaattgt gggacatcgc ctataatttg cattatcata
    26401 cctgtcattc aatgccgtgc ctaacaccac tgacgctatt cggacaggat taacactagc
    26461 gtagagatga cgagtacgtt agtctctgac cgtttgtcac gcgcaacgtt accgatgatg
    26521 gaacaataaa atcaacgtat gaaaaaacgt atccccactc tcctggccac catgattgcc
    26581 accgcccttt atagtcaaca gggactggca gccgacctcg cctcacagtg catgttgggc
    26641 gtgccaagct atgaccgtcc tctggtacag ggcgatacca atgacttacc cgtgactatc
    26701 aatgctaacc acgcgaaagg ggactacccg gatgacgccg tgtttactgg cagcgtggat
    26761 atcatgcagg gtaacagccg tctgcaggcc gacgaagtgc agctccatca aaaagaggca
    26821 ccaggacaac cggagccggt acgtaccgtt gatgcgctcg gtaatgtcca ttacgacgat
    26881 aaccaggtga tcctcaaagg gccgaaaggc tgggcgaatc tgaacaccaa agataccaac
    26941 gtctgggaag gtgattacca gatggtgggt cgccagggtc gcggtaaagc ggacctgatg
    27001 aaacaacgtg gcgaaaaccg ctataccatt ctggataacg gtagctttac ctcctgtctg
    27061 ccgggttctg acacctggag cgtggtaggt agcgaaatta ttcatgaccg cgaagaacaa
    27121 gttgcggaga tctggaacgc ccgctttaag gtgggtccgg tatcgatctt ttatagcccc
    27181 tatttgcagt tgccggtggg tgacaaacgt cgctctggtt tcttgatccc gaacgccaag
    27241 tacaccacca ccaactactt tgagttctac ctgccatatt actggaacat cgcgccaaat
    27301 atggatgcca ccatcacgcc gcattatatg catcgtcgtg gcaacatcat gtgggagaac
    27361 gaattccgct acctctccca ggcgggcgct ggcttgatgg aactggacta tctgccttca
    27421 gataaagtct atgaagatga acacccgaac gatgacagtt cacgtcgttg gttattctac
    27481 tggaaccact ccggggtcat ggatcaggtg tggcgtttca acgtcgacta caccaaggtc
    27541 agcgatccta gctacttcaa tgatttcgat aacaagtacg gttccagtac tgacggctac
    27601 gcaacgcaaa aattcagcgt tggctatgcg gtgcaaaact tcaatgccac cgtttcaacc
    27661 aagcagttcc aggttttcag cgaacagaac accagtagct actcggcaga gccgcagtta
    27721 gacgttaatt actaccagaa tgatgttggt ccgtttgata cgcgtattta cggccaggca
    27781 gtgcactttg ttaacaccag agacgacatg cctgaagcaa cccgtgttca cctggaaccg
    27841 accatcaatt tgccgctctc taataactgg ggcagcatca ataccgaagc gaagttgctg
    27901 gcaacccatt atcagcaaac caatcttgac tggtataact ccagaaacac gaccaagctg
    27961 gacgaatccg ttaaccgcgt aatgccgcaa ttcaaagttg acggcaaaat ggtctttgaa
    28021 cgcgatatgg aaatgctggc tccgggttat acccaaacgc tggaaccgcg cgcgcagtat
    28081 ttgtacgtgc cgtatcgcga tcagagcgac atctataact acgactcgtc tctgctgcaa
    28141 tctgactact ctggcctgtt ccgggaccgg acttacggcg gtcttgaccg tattgcctcc
    28201 gctaaccagg tgacgaccgg tgtcacatct cgcatatatg atgatgctgc cgttgaacgt
    28261 tttaatattt ccgttggtca aatctactat ttcacggagt ctcgcactgg cgatgacaac
    28321 ataacatggg agaatgacga caaaacgggt tcactggtgt gggcaggcga tacttactgg
    28381 cgtatctccg agcgttgggg attgcgtggc gggattcagt acgatacacg tctggataac
    28441 gtagcgacca gtaactccag cattgaatac cgtcgggatg aagaccgtct ggtacagctg
    28501 aattaccgtt acgccagccc ggaatatatt caggctacgc tgcctaagta ctattccact
    28561 gctgagcaat ataagaatgg tatttcgcag gtaggtgctg tcgccagctg gccaattgcc
    28621 gatcgttggt ccattgttgg ggcctactac tacgacacca atgcgaacaa gcaagccgac
    28681 tctatgttag gtgtgcaata cagctcctgc tgctatgcaa ttcgcgtcgg ttacgagcgg
    28741 aagctgaacg gttgggataa cgataaacaa catgcggtat atgacaacgc aatcggcttt
    28801 aacatcgaac ttcgcggcct gagctccaac tacggtctgg gtacgcaaga gatgctgcgt
    28861 tcgaacattc tgccgtatca aaacactttg tgatttgttg atttaccacg taatccgcag
    28921 tgcggttaat tgaaatggaa aaagtatgaa gaactggaaa acgctgcttc tcggtatcgc
    28981 catgatcgcg aataccagtt tcgctgcccc ccaggtagtc gataaagtcg cagccgtcgt
    29041 caataacggc gtcgtgctgg aaagcgacgt tgatggatta atgcagtcgg taaaactgaa
    29101 cgctgctcag gcaaggcagc aacttcctga tgacgcgacg ctgcgccacc aaatcatgga
    29161 acgtttgatc atggatcaaa tcatcctgca gatggggcag aaaatgggag tgaaaatctc
    29221 cgatgagcag ctggatcagg cgattgctaa catcgcgaaa cagaacaaca tgacgctgga
    29281 tcagatgcgc agccgtctgg cttacgatgg tctgaactac aacacctatc gtaaccagat
    29341 ccgcaaagag atgattatct ctgaagtgcg taacaacgag gtgcgtcgtc gcatcaccat
    29401 cctgccgcag gaagtcgaat ccctggcgca gcaggtgggt aaccaaaacg acgccagcac
    29461 tgagctgaac ctgagccaca tcctgatccc gctgccggaa aacccgacct ctgatcaggt
    29521 gaacgaagcg gaaagccagg cgcgcgccat tgtcgatcag gcgcgtaacg gcgctgattt
    29581 cggtaagctg gcgattgctc attctgccga ccagcaggcg ctgaacggcg gccagatggg
    29641 ctggggccgt attcaggagt tgcccgggat cttcgcccag gcattaagca ccgcgaagaa
    29701 aggcgacatt gttggcccga ttcgttccgg cgttggcttc catatactga aagttaacga
    29761 cctgcgcggc gaaagcaaaa atatctcggt gaccgaagtt catgctcgcc atattctgct
    29821 gaaaccgtcg ccgatcatga ctgacgaaca ggctcgtgtg aaactggaac agattgctgc
    29881 tgatatcaag agtggtaaaa cgacttttgc tgccgcagcg aaagagttct ctcaggatcc
    29941 aggctctgct aaccagggcg gtgatctcgg ctgggctaca ccagatattt tcgatccggc
    30001 cttccgtgac gccctgaccc gcctgaacaa aggtcaaatg agtgcaccgg ttcactcttc
    30061 attcggctgg catttaatcg aactgctgga tacccgtaat gtcgataaaa ccgacgctgc
    30121 gcagaaagat cgtgcatacc gcatgctgat gaaccgtaag ttctcggaag aagcagcaag
    30181 ctggatgcag gaacaacgtg ccagcgccta cgttaaaatc ctgagcaact aatggttaaa
    30241 acccaacgtg ttgtgatcac tcccggcgag cccgccggga ttggcccgga cttagttgtc
    30301 cagcttgcac agcgtgagtg gccggtcgaa ctggttgttt gtgccgatgc cactctcctt
    30361 accgaccggg cagcgatgct cggtttgccg ctcaccctcc gcccttattc ccccaactcc
    30421 cctgcacaac cgcaaactac gggcacatta acgctacttc ctgtcgcgct acgtgaatct
    30481 gtcactgcgg ggcagttagc gattgaaaat gggcattacg tggtggagac gctggcgcga
    30541 gcgtgtgatg gctgtctgaa cggtgaattt gctgcgctga tcacaggccc cgtgcataaa
    30601 ggcgtcatta acgacgcagg cattccgttt accggtcata ccgagttttt cgaagagcgt
    30661 tcgcaggcga aaaaagtggt gatgatgctg gcgaccgaag aacttcgcgt ggcgctggca
    30721 acgacgcatt taccgctgcg cgatatcgca gatgctatca cccctgcact tttgcacgaa
    30781 gtgattgcta ttttgcatca cgatttgcgt acaaaatttg gtattgccga accgcgcatt
    30841 ctggtctgcg ggctgaatcc gcacgcgggc gaaggcggtc atatgggtac ggaagagata
    30901 gacaccatta ttcctgtgct cgacgagctg cgggcgcagg ggatgaaact caacgggccg
    30961 ctgcctgccg ataccctgtt tcagccgaaa tatctcgata acgccgacgc cgtgctggcg
    31021 atgtaccacg atcagggtct tcccgtgcta aaataccagg gcttcgggcg cggtgtgaac
    31081 attacgctgg gcctgccctt tattcgcaca tcagtggacc acggcaccgc acttgaactg
    31141 gcgggacgtg gcgaagccga tgtcggcagt tttattacgg cgcttaatct cgccatcaaa
    31201 atgattgtta acacccaatg aataatcgag tccaccaggg ccacttagcc cgtaaacgct
    31261 tcgggcaaaa ctttctcaac gatcagttcg tgatcgacag cattgtttct gccattaacc
    31321 cgcaaaaggg ccaggcaatg gtcgaaatcg gccccggtct ggcggcattg accgagccgg
    31381 tcggcgaacg tctggaccag ctgacggtca tcgaacttga ccgcgatctg gcggcacgtc
    31441 tgcaaacgca tccattctta ggcccgaaac tgacgattta tcagcaggat gcgatgacct
    31501 ttaactttgg cgaactggcc gagaaaatgg gtcagccgct gcgtgttttc ggcaacctgc
    31561 cttataacat ctccacgccg ttgatgttcc atctgtttag ctatactgat gccattgccg
    31621 acatgcactt tatgttgcaa aaagaggtgg tgaatcgtct ggttgcagga ccgaacagca
    31681 aagcgtatgg tcgattaagc gtcatggcgc aatactattg caacgtgatc ccggtactgg
    31741 aagttccgcc gtcagccttt acaccaccac ccaaagtgga ttccgccgtc gtgcgcctgg
    31801 ttcctcatgc aacgatgcct cacccggtta aagatgttcg tgtgttgagc cgcatcacca
    31861 ccgaagcctt taaccagcgt cgtaaaacca ttcgtaacag cctcggcaac ctgtttagcg
    31921 tcgaggtgtt aacgggaatg gggatcgacc cggcgatgcg agcggaaaat atctctgtcg
    31981 cgcaatattg ccagatggcg aactatctgg cggagaacgc gccttttcag gagagttaac
    32041 gatgatcaat tcgccccgag tgtgtattca ggttcaaagc gtctacattg aggcacaatc
    32101 ttcacctgat aatgaacgtt acgtttttgc ttataccgta accatacgca atctggggcg
    32161 agcgccagtg cagttgttgg ggcgttactg gctgatcacc aatggcaatg gccgtgaaac
    32221 cgaagtccag ggcgaaggag tggttggcgt ccagccactt atcgcgcctg gcgaagagta
    32281 ccagtacacc agcggtgcaa tcattgaaac cccgctgggc accatgcagg gtcactacga
    32341 aatgatcgat gaaaatggcg tccctttcag catcgacatt cccgtattcc gactcgccgt
    32401 tcccacactc attcattaaa agaatatggc gacatacctt attggcgacg ttcatggttg
    32461 ttacgatgaa ctgatcgcat tgctgcataa agtagaattt acccctggga aagataccct
    32521 ctggctgacg ggcgatctgg tcgcgcgcgg cccgggttcg ctggatgttc tgcgctatgt
    32581 gaaatcctta ggcgacagcg tacgtctggt gctgggcaat cacgatctgc atctgctggc
    32641 agtatttgcc gggatcagcc gcaataaacc gaaagatcgc ctgacaccgc tgctggaagc
    32701 gccggatgcc gacgagctgc ttaactggct gcgtcgccag cctctgctgc aaatcgacga
    32761 agagaaaaag ctggtgatgg cccacgcagg gatcacgccg cagtgggatc tgcagaccgc
    32821 caaagagtgc gcgcgcgatg tagaagcggt gctgtcgagt gactcctatc ccttctttct
    32881 cgacgctatg tacggcgata tgccaaataa ctggtcaccg gaattgcggg ggctgggaag
    32941 actgcggttt atcaccaacg cctttacccg tatgcgtttt tgcttcccga acggtcaact
    33001 ggatatgtac agcaaagaat cgccggaaga ggcccctgcc ccactgaaac cgtggtttgc
    33061 gattcctgga cctgtcgctg aagaatacag catcgccttt ggtcactggg catcgctgga
    33121 aggcaaaggt acgccggaag gtatttacgc gctggatacc ggctgctgct ggggtgggtc
    33181 attaacctgc ctgcgctggg aagataaaca gtattttgtc cagccgtcga accggcataa
    33241 ggatttgggt gagggagagg cggtcgcgtc ttaaatcccg tcgagcataa aatctaatgc
    33301 tcctttaatc aacgttcggt gcgcgctggc atcgcaaaat ttcgttccca gcgcgttaac
    33361 tggaacagtt gcagtctcat gcgtcatgag cacatattct ttgccgtcta ccagtaaaag
    33421 gattgggtta agccgttctg gtgggcgaat acggctaaat cgttcaatag gtgttaatgg
    33481 aataacaata cggcgattaa tcacaccaat aatgtcgctg gtaacatcga taacaaaggg
    33541 aaacgcggcg ttcctgctgc gactgcggta taccgtaaat tgcatcgcta gaagctccgg
    33601 tactcatcag agaaacatcc ggtttcatca gccaattgat ttaacgcagc gatagcttca
    33661 gcgttctctt cacgccaacg tgttgctgca tgttttttaa gttcagcatt gagcgcaacc
    33721 gtaagggtgg cgctcatatt taagcctgca tcacgagcgc gattgactaa ctcacggtcg
    33781 acggtgacgg tcacactttg tgtggtacgt tttgcagtca tgatatcgtg ctcctgtaca
    33841 atttatacgt ctagtataca cggatatctg cacgtatcag tgatgccgga attctaatat
    33901 acaaaattac cgccgctcca gaatctcaaa acaatagctg tgagagttct gcgcatcagc
    33961 gtcgtggaat tcgctgaata ccgattccca gtcatccggc tcgtaatccg gaaaatgggt
    34021 gtcgccttcc acttctgcgt cgatatgcgt caggtacagt ttttgcgctt tcggcaggaa
    34081 ctgctcataa acgcgaccgc cgccaatcac catgatttct ggtacgtcac cacacgccgc
    34141 gatggcttca tccaccgact tcacccacgt tacgcgatcg tccgtacccg gttgactgct
    34201 gaggataata tttttgcgtc ctggcaacgg acgaccgatg gattccctgg tatggcggcc
    34261 cataattacg ggtttattta aggtgttgcg tttaaaccag gcgagatcgg caggcaggtt
    34321 ccacggcatg gcgttttcca tgccgataac gcgatctacc gctaacgccg caatcagact
    34381 gatcattgag atttcccgat aaaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc
    34441 gtcgactggt gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct
    34501 ctctccctgg actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg
    34561 atttacgtca ctctttatta ggatgagggt ttcgtttccg gttcatccgc catgttgccg
    34621 gtatgtttac cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg
    34681 cggtcctcgg tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg
    34741 gctttggtgt cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg
    34801 aacacatcgg cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga
    34861 cgcccggttt tcagcgcacc ttcaaaggtt tcacgctctg gcttttcgac gcctgcctga
    34921 cgcaaacaaa tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa
    34981 tgttctttca ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat ggcgttaatc
    35041 agcacttccg ctttcgccgc tcccgcagat tccagtaaat ccatccgcgt ggcatcgcca
    35101 taaaacactt tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc
    35161 accaccattt tcaccccgct ggagagcagt aaacgtccgg tgatctgccc gaaacgaccg
    35221 aatccagcaa tgattacccg cggctgttct tcgtcgatct catcggcttc acgcgcctct
    35281 tcagtagaag attgctcaag gcgattgagg atcaccagca gaatcggcgt tgccgccatc
    35341 gacagcgcca ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc
    35401 tgcgccgcgc caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac
    35461 cagcgacgct gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt
    35521 ttgatgatga ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg
    35581 ccaaagtcta tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaag
    35641 ggttcgatat cgctttccag cgcatgacgg tattccgagc ttgccagcag tacgcccgcc
    35701 agaaacgcgc ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac
    35761 acaaggaata acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc
    35821 agcgccggac gcgtgacata gcgccccagc agtaccacca gcaccagcgc gcccgccact
    35881 tttaacgccg agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc
    35941 gggatcatcg ccaccagcgg gattgccgcg atatcctgga acagcagcac cgcaaaggca
    36001 ctgcggccca tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc
    36061 gtagaggaga gcgccagcgt catgccgatc aattccgcga cctgccagcg cagcccaagt
    36121 aacatacaga acagccccag caggccgccg caaatcacca tctgcaatgc gccaccgcca
    36181 aacactgccg cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac
    36241 agcatcagca ccacgccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt
    36301 cgcagccccc acgggccaat aatgcagtcg gcgatcaggt agccaagtac cgatcccaga
    36361 ccaagacgta ctgcaatggg tacaatcagc gccgccgaac cgagataaat cagcgcctga
    36421 accagcgtat ggctatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg
    36481 acgcgcctgc ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg
    36541 tggcagccag ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc
    36601 aaagcccgga tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca
    36661 cagcaaatgt ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgagaa
    36721 gactttatcg atccaaagtt taaggagcgg aggaatgctg taccactgca tcgggtgctg
    36781 ccagacgatc agatcggcgc gagacagcgc ctcctgctcg gcggcaatat cgatattgaa
    36841 gtcaggatag agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc
    36901 aaacatccgt ttattcgcat gggaatgatg cggatacgga tgcgcataaa ttataagaat
    36961 cataggtagc ccgcgtgttt acgcctttgt tttcccacaa agtgtagtca tatattcacg
    37021 cggcttatag tcaataatat tgattcagaa aagagataaa tctgaacaaa ttaccccatt
    37081 ctctgcccgc ggatcgtcat attgctggtc tgcgccagtg acatccctcg agtctccggg
    37141 gcaaacgcta cggaaatcaa caagccaaac agcgagatac ccgcccccat tagcatcgtg
    37201 ttactgatac cgtaattatt gataaagatc ggtagtgccc aggtcgaaac aatggtgcca
    37261 atacgactta aggacataat cacgcccacg gcagaggcgc ggatatccgt cgggaagagt
    37321 tcattaggat agagccactg caaattaccc ggcccgccag agaaaaaggc atacaccgca
    37381 aaggccatca ctaccagcca gatccccata tccgggatca gccccaaaac cgccagcgcc
    37441 agcgtcatca tggcaaagct gccaatcaac aatggacgcc gtccggcagt gtttagccac
    37501 agcatcggcg gaatacagcc gagcataaag aacaggctaa tcaccacgtt ccccagtgcc
    37561 gcgtttttgc caacacccaa tcccaacaaa ccaacgattt gcgggccaaa ggtgtaaatg
    37621 gcgaacattg ggatcacctg gcaggtccag atggcggcaa caaacagaac aaaaggaaaa
    37681 tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc atcgaaagcc
    37741 accggttcgc caaacagttt gatcatcatc tcttcgcact cttttactcg ccctttgcgt
    37801 aataaccagc ggggagattc aggcagttcg aatcgaccaa tcaaaatcaa caaacagggg
    37861 atcgccgcgc tacccagcat ccagcgccag ccgccttcca catcataaag ccagtagccg
    37921 accagatcgg cacaggtcgc gccgacatac cacatggcgg cgataaagct gatggaaaac
    37981 gcccgctgac gggtactgga gaactcagtg atcatcgaag tggcgatggg atagtctgca
    38041 ccgatgacaa tgccgataag tacccgcatc accaacagtt cgacggggga tgaaacaaac
    38101 atcgtcgcca ccgatatcac gccgatggcg atgatatcaa tgaggaacat tttgcgccgt
    38161 ccgactttat cggaaatata accaaacagc gatgtgccaa cgaacagccc ggcgagcgtt
    38221 cccgcgccca gcaagccaat ccagtcagcg tccagtttta gcgccggcgt cagttgctcc
    38281 agcgccacgc caatcattac cagtatataa ccatccagaa acggaccacc gcttccccac
    38341 agcaaaatgc ggcggtgaat agaggaaaat ttaagatcgt caaagtttct ggacggttgc
    38401 atgggttatt cctgtttttt gcaatgagag tcagtgcggc gttacgtatc aaaccaacat
    38461 cagccgtaac ggaactccac accaaaggtg ccgcgcgggt attcccactg ttccagcgcc
    38521 gaccccagcc ccagaatgcg acaggtgccg cactccagac atccggcgta atcgaagcgc
    38581 acactgccgt catcctgctt cttgtacaga cctgcggggc acgctttcac cagcagctcc
    38641 agcgcctgtt tatcggcatc ggctttcaca acaatgtgcg gatgctcttc atcgacattg
    38701 aatttattga cgcccagttt gacgtccaca ttgacgggag aagtcataaa acggtcactc
    38761 ctttcatgcc atccttgatc agattgatga agcccacttt cttgccgtgg cggaggattt
    38821 tcttgcgcat cagttccggc gcactgccgt caatggtgaa caggtcacgc gccacgccta
    38881 ccgccagttc cgggtagccg ctaaacatgc gtgggttatc aaggaaggcc ggtagtttct
    38941 ggtacatacg catatcgcgc agcgggccac tctcaagatg ctgacgatat tccgcgagtt
    39001 tttgcttact gaaatcgtcg cttttcatcg ctgaaagcac ggtttttgct gcagcttccc
    39061 cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc gaggttcata cacattccgg
    39121 cggcatcacc ggcaatcaat acgccgtcac cgaccagctc cggcagcatg ttgatgcctg
    39181 cttccggcac tacgtgagcg gaatattcca ccagcttgcc acccgcgatc agcggtgcaa
    39241 cggccggatg ttgtttgaaa tcttccagca tttgcggcac cgattttttc gcgtcatgca
    39301 gatgatgcag accacaaacc agccccagcg acagggtgtt ttcattggta taaagaaaac
    39361 cgccgcccat caggccatcg gtgggtgatc ccgcaaatag gcaagccgcc ccctgattac
    39421 cctgcaactg aaaacggtct tcaataaccg acttcggtaa ctcgatcagt tccttcacgc
    39481 caaccgccac attcgtcggt ttaacgcgtt ttgccatccc cagcttttcg gcaaggatgg
    39541 agttcactcc atcagcaagg atcaccgttt tcgcttcaat cacatcgcca tcggcttcta
    39601 caccgacgac tttgccatcg cgctgtacga ggttatcgac gcggatcccg gtaattaact
    39661 gcgcgcccgc ttcttcggcc tgctccatca gccaggcatc aaatttactg cgcaaaacgg
    39721 agtaagaacg ctgggatggc gaggtttcgt caccgttgca gtagtccata gtcatcgctg
    39781 acttttccgt cataaacgcg agtttttcat gggtgatcag gcgttctacc ggggcggatt
    39841 cggcgaaacc aggaataatg tgttccaggc tgtgggcata gagacgcccg ccggtgacgt
    39901 tcttggcacc tgcggaattg ccacgctcga taactaacac ttgcgcccct tcgcgggcga
    39961 gcaccagtgc ggcaaccgaa ccggcaagcc ctgcaccgac gatgatggcg tcaaagatat
    40021 cttcggacat aagagctccc tgtcagcggc aggaccaaaa tgcagccctg ccagagtgga
    40081 tcaacgcgct aaagctgcgg tcagcgccgg aaggatcttc acggcgtcgc caacaatgcc
    40141 gtaatccgcg tactggaaga tcggcgcatt tttatcttta ttgatggcga aaatggtttg
    40201 cgacgcgtta gcgccaacca tgtgctggat ctgcccggag atccccacag ccaggtacag
    40261 ttcaggtttc agcatcaggt tggagatacc gacatagcgt tcgtgctcca tccatttttc
    40321 gttttctgcc accggacgag aacaggccaa ttccgcacct atcgccttgc aaagctgttc
    40381 tgccagcgca atgttctctt tgctgccaat accgcgaccg acgctgacca ccaggcgggc
    40441 tttgtcgaga tcgacgctgt tgctctggcg cgcctgggtt gccgtgcggg taatcgccac
    40501 agccggagcc tgccactcca cggtgtgcgt ttcgccagtg cgtgacgcgt ctggctgagc
    40561 cgcatcgaac gtgccgctgc tgatggtcag taccgcatac ggcgtggcaa tgcgttcttc
    40621 gccaatcgcc agaccaccgt aaaccatatg tttcactgtt gctttaccgt cctgtacgct
    40681 gacggtgctg gcatcgttag acaccgccgc tttaaggcga taacccagtt ttgccgccag
    40741 taatttgccg cgacgggtgt ttggcaacag caccaggccg tctgcaccgt gctggcgaat
    40801 agtgtcagcc atgacactgg cgtaatcttc gatcatccga tcgtccggtt tgccgtttaa
    40861 tttccagaca tgattagcgc cgagctggat tgcctgtgcg ccgtcggcat cattgaggac
    40921 aaaggcattg atttgattag ctaaagcctg cgcaccgttc atcagttccg gcagacgaga
    40981 aggggtatcg ctgaataccc agacttgaga aaacgtgttc atagcatccc ctgtaattaa
    41041 atgactttgc gaagattttc agcaaatgcg gcgatctgtt cttcgccgtc gccttcaatc
    41101 acgatgcgct gacgctcgcg ctgtttcggc gcggcaacct gttgttctga ccatgctgcc
    41161 tctgcgttaa aaccgatatc cgccgccgac catacctgga ctggcttttt cgccgcgccg
    41221 agaatggctt tcatcgaagg aatttgtggg gagttgatat cagtggaaac agcaacaacc
    41281 gcaggcagtg gaatgcttaa ggtttcggtt tcatcttcca gttcgcgctc aacggtgagg
    41341 gtatctgccg tcagggagat aattttgctg acgccgttaa ctgccggaat attgaggatt
    41401 tcgcctacca gcaaaccaac ctgctgggca taaaggtcgg aagaaccatc gccacagagg
    41461 atcaaatcaa agcctgcttt ctgggcggct gcagccagtg cgctcgccgt ttgttgcggc
    41521 agtgcctgct cgaactggtc atcaatcacc acaatcagtt catccggacc gcgcgatagc
    41581 acatctttac gccctttggc gttggtcagg gctttaccgc ccacacttag ggccgtcacc
    41641 tgcgcctctg ctgcctgttg ctttagctgg caagccgctt caatagcgtt gagatcgtat
    41701 tggcttattt tggcatcggc tttactgaag tctaacgaac catcagcatt attgaccgca
    41761 atatcctgtt catcaggcac gcacttatag caagtaataa tcttcattgc atctccagaa
    41821 atcatgaagg aaacgtcgtt catatcggtg gtgttaatta cagatatcat gccctttaat
    41881 ttggaaataa aactttaatc accaatattg aaaatgtcac acgcagataa atccaacttt
    41941 caatattgtt aagttcctca ccaatattga aaacacggcg tagcaaaaag aaattttcaa
    42001 tattgtttta tggatcacca atattgaaag tcagatgcta tttttttact cgcaacataa
    42061 cataacataa caaactgatt aataatcaaa attaacgaga aaagcgccct ctattcaccc
    42121 gaaacaaaaa tgtgataaca atcacagaat gcagcttatt gaatacccat tatgagttag
    42181 ccattaacgc gtccatgagg ttaataataa ttatattaaa tgttaacaaa aataaaacaa
    42241 acgggaacgc aaaataaata ttcgttttca cagtggaatt aactcatgaa gaatgaaaag
    42301 agaaaaacgg gaatagaacc gaaggttttc tttccgccat taataatcgt cggcatactt
    42361 tgttggctta cagtcagaga tctggatgcg gcgaatgtcg ttattaatgc tgtattcagt
    42421 tacgtcacca atgtatgggg atgggcattt gaatggtata tggtggtgat gcttttcggt
    42481 tggttctggc tggtgtttgg cccgtatgcc aaaaagcgtt taggtaacga accacctgaa
    42541 tttagtaccg ccagttggat ctttatgatg ttcgcctcct gtacgtctgc tgccgtactg
    42601 ttctggggat cgattgagat ctactactac atctccaccc cgccgtttgg cttagaaccg
    42661 aactcgacag gagcgaaaga gttggggctg gcttacagct tgttcctctg gggacctctg
    42721 ccgtgggcca cttacagctt cctttcagtc gccttcgctt acttcttctt tgtccgcaaa
    42781 atggaagtga ttcgccccag ctccaccctg gtgccgctgg taggtgaaaa acacgccaaa
    42841 gggttgttcg gcactatcgt cgacaacttc tatctcgtcg ccttgatctt cgcgatgggt
    42901 accagtctgg gccttgccac gccgctggtg accgagtgta tgcaatggtt gtttggcatt
    42961 ccgcataccc tgcaactgga cgctatcatc attacctgct ggattatcct caacgccatt
    43021 tgcgtcgcct gcggtctgca aaaaggggta cgtatcgcca gtgacgtgcg tagttacctg
    43081 agcttcctga tgctgggttg ggtgttcatc gtcagcggtg ccagcttcat catgaactac
    43141 ttcaccgatt cggtggggat gttgctgatg tatctgccgc gcatgttgtt ctataccgat
    43201 cccatcgcta aaggcggctt cccgcagggc tggaccgtgt tctactgggc atggtgggtg
    43261 atttacgcca tccagatgag tatcttcctc gcccgcatct cccgtggtcg tactgtgcgt
    43321 gaactgtgct tcggcatggt gatgggactg acagcatcca cctggatcct gtggactgta
    43381 ctcggtagta acactctgct gttgatagat aaaaacatca tcaacattcc aaatctgatc
    43441 gaacagtacg gtgtggcgcg cgccatcatc gaaacctggg ccgctctgcc gctcagcacc
    43501 gccaccatgt ggggcttctt catcctctgc tttattgcca ccgttacgct ggttaacgcc
    43561 tgctcttata ccctggcgat gtccacttgc cgcgaagtac gcgatggtga agaaccaccg
    43621 ctgctggtgc gtatcggttg gtcaattctg gttggcatta tcggtattgt tttgctggcg
    43681 ctcggcggcc tgaaaccgat tcaaactgcc attatcgccg gaggatgccc gctgttcttc
    43741 gtcaacatta tggtgacgct ctcctttatt aaagacgcga aacagaactg gaaagattaa
    43801 ttaaccccca aaatatcaag aggttgaaag atggatttta atttaaatga tgagcaggaa
    43861 ctgtttgtcg ccggtatccg cgaactgatg gccagcgaaa actgggaggc ctattttgcc
    43921 gagtgcgacc gtgacagcgt ctacccggaa cgttttgtca aagcactggc ggatatgggt
    43981 atcgacagtc tgctgatccc tgaagagcac ggtggtctgg acgcggggtt tgttactctc
    44041 gccgccgtgt ggatggagct gggacgtctg ggggcaccaa cctatgtact gtaccagttg
    44101 ccgggcgggt tcaatacctt cctgcgcgaa ggcacacaag agcagatcga caagattatg
    44161 gctttccgcg gcaccggtaa gcagatgtgg aactcagcga ttaccgaacc gggcgcgggc
    44221 tccgacgtgg gtagcctgaa aacgacttat acccgtagaa atggtaagat ttatcttaat
    44281 ggtagtaagt gttttattac cagtagcgcc tacaccccgt acatcgtggt gatggcgcgc
    44341 gacggggctt ctccggacaa acctgtctac accgaatggt ttgttgatat gagcaaaccg
    44401 ggcatcaaag tgaccaaact tgagaagctc ggtctgcgta tggatagctg ctgtgaaatc
    44461 acctttgacg atgtggaact ggacgagaaa gacatgttcg gtcgggaagg taacggcttt
    44521 aaccgcgtca aagaagagtt cgaccatgaa cgtttcctgg tagccctgac caactacggt
    44581 acggcgatgt gcgcctttga agatgcggcg cgctacgcca accagcgcgt gcagtttggc
    44641 gaggctattg gtcgtttcca gttgattcag gaaaaattcg cccacatggc gatcaaatta
    44701 aactccatga aaaacatgct gtatgaagca gcgtggaaag cagacaacgg caccatcacc
    44761 tctggcgatg cagcgatgtg caaatacttc tgcgccaatg cggcatttga agtggtggat
    44821 agcgcaatgc aggtgctggg cggtgtcggg attgcgggca accaccgcat cagccgcttc
    44881 tggcgtgacc tgcgtgtaga ccgcgtttcc ggaggatctg acgaaatgca gatcctgacg
    44941 ctgggtcgtg cggtgctgaa gcaataccgc taagtccgtg cgctgcctga tgcgacgctg
    45001 acgcgtctta tcaggcctac gggagcacga atgtaggccg gataaggcgt ttacgccgca
    45061 tccggcagtc atgcgctaca taaatttttc aggagctaat aatggatcat ctacccatgc
    45121 cgaaattcgg gccgttggcc ggattgcgcg ttgtcttctc cggtatcgaa attgccgggc
    45181 cgttcgccgg gcaaatgttc gcagaatggg gtgcggaagt tatctggatc gagaacgtcg
    45241 cctgggccga caccattcgc gttcaaccga actacccgca actctcccgc cgcaatttgc
    45301 acgcgctgtc gttaaatatt ttcaaagatg aaggccgcga agcgtttctg aaattaatgg
    45361 aaaccaccga tatcttcatc gaagccagta aaggtccggc ctttgcccgt cgtggcatta
    45421 ccgatgaagt actgtggcaa cacaacccga aactggttat cgctcacctg tccggttttg
    45481 gtcagtacgg caccgaggag tacaccaatc ttccggccta taacaccatc gcccaggcct
    45541 tcagtggtta cctgattcag aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata
    45601 ccgccgatta cttttctggc ctgaccgcca ccacggcggc gctggcagca ctgcataaag
    45661 cgcgtgaaac cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg atgctgcgta
    45721 tgggccagta cttcatgatg gattacttca acggcggcga aatgtgcccg cgcatgagca
    45781 aaggtaaaga tccctactat gccggttgcg gtctgtataa atgtgccgac ggctacatcg
    45841 tgatggaact ggtgggcatt acccaaattg aagagtgctt taaagatatt ggcctcgcac
    45901 atctactcgg tacgccggaa atcccggaag gcactcagct tatccaccgt atcgaatgcc
    45961 cttacggccc actggtagaa gaaaaactcg atgcctggct ggcggcacat accatcgcag
    46021 aagttaaaga acgctttgcc gaactgaata tcgcctgtgc caaagtactg accgtaccgg
    46081 aactggaaag caatccacag tatgttgccc gtgaatcaat cactcagtgg caaacgatgg
    46141 atggtcgcac ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac cccggacaaa
    46201 tctggcgcgg aatgccctca catggcatgg acacggctgc cattttgaaa aatatcggct
    46261 acagcgaaaa cgacattcag gagttggtca gcaaaggtct ggccaaagtt gaggactaaa
    46321 cgcgtggcgt cagtcggctt gcctgactag cgcccgccca ataacaaacg aacaaaaatg
    46381 gatagaggtg caatggatat cattggcgga caacatctac gtcaaatgtg ggacgatctg
    46441 gcggacgttt acggtcataa aacggcgctg atttgtgaat ccagcggcgg agtcgttaac
    46501 cggtatagtt atcttgagtt aaatcaggag attaaccgca cggcaaacct gttttatacg
    46561 ctggggattc gcaaaggcga caaggttgca ctacatctcg acaactgccc ggaatttatc
    46621 ttttgctggt tcgggctggc aaaaattggc gcgattatgg tgccgattaa cgcccgcctg
    46681 ttacgcgagg aaagtgcgtg gatcctgcaa aatagccagg cgtgcctgct ggtgaccagt
    46741 gcgcaattct atcctatgta tcaacagatt cagcaagaag atgccactca attgcggcac
    46801 atttgcctga cagatgtggc acttcccgct gatgatggcg tgagttcgtt tactcaactg
    46861 aaaaatcaac aacctgccac cttgtgctat gcaccgccgc tatcgactga cgatacggcg
    46921 gaaattctct tcacctccgg caccacctcc cgaccgaaag gtgtggtgat tacccattac
    46981 aacctgcgct tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc
    47041 tacctgacgg taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg
    47101 ttttctgccg gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga
    47161 caggtacaga agtaccgcgc caccattacc gaatgtattc cgatgatgat tcgtacgttg
    47221 atggtgcagc cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt gatgttttat
    47281 ctcaacttgt cggagcagga aaaagacaca ttttgtgaac gcttcggtgt tcgcttgctg
    47341 acgtcttatg ggatgacgga aaccattgtg ggcattatcg gcgatcgccc tggcgataaa
    47401 cgacgctggc cgtcgattgg tcgggcgggg ttttgctacg acgcggagat ccgcgacgat
    47461 cacaatcgcc cgctcccggc aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg
    47521 aaaaccatct tcaaagagta ttttctcaac ccgaaagcca ctgcaaaagt gctggaagct
    47581 gatggctggc tgcataccgg cgataccgga taccgcgacg aagaaggctt tttttatttc
    47641 atcgatcgcc gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg
    47701 gaaaatatca tcgccaccca tccaaaaatt caggatatcg tggttgtggg tattaaagat
    47761 tcgattcgcg atgaagccat caaagcattt gtggtgctga atgaaggtga aacattgagc
    47821 gaagaggaat ttttccgctt ctgcgaacaa aatatggcga aatttaaagt gccctcttat
    47881 ctggagatca gaaaagatct gccacgtaat tgctcgggga aaataattag aaagaatctg
    47941 aaataaatga aacggcaggg aactaccctg ccagctaata atcacgctct aaaacaatat
    48001 gcattttttg cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt
    48061 gaatcattac atctgacccg caatggatca attctggaaa ttactcttga tcgcccaaaa
    48121 gcgaatgcta ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt
    48181 gacgatccgc aattacgtgt cgccattatt accggtgccg gagagaaatt cttttccgcg
    48241 ggctgggatt taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccgggt
    48301 ggttttgcgg gattaaccga aattttcaat ctcgacaaac cggttatcgc agctgtgaac
    48361 ggctacgcct ttggcggcgg tttcgaactg gcgctggcgg cagattttat tgtttgtgcc
    48421 gataacgcca gcttcgccct gccggaagcc aaactgggca tcgttcctga cagcggcggt
    48481 gtgctgcgtc tgccgaagat cctgccgcct gccatcgtca atgaaatggt gatgaccggc
    48541 agacgaatgg gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag
    48601 gcggaactga tggataacgc ccgcgaactg gctcagcagc tggttaacag cgccccgctg
    48661 gcgattgcgg cgctgaaaga gatctaccgc accaccagcg aaatgccggt agaagaatcg
    48721 tatagctata ttcgcagcgg cgtgttgaaa cactatccat cggttctgca ttcggaagat
    48781 gccattgaag ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa
    48841 caaccgtgag ctattacgcc tttgaggggt taattccggt ggttcacccg acggcgtttg
    48901 tccatcccag tgccgtcttg attggcgatg tgattgtggg agccggtgtc tacatcggcc
    48961 cactcgcctc actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc
    49021 aggatggctg cattatgcat ggctacaccg acactgacac catcgttggg gaaaacggcc
    49081 atatcgggca cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga
    49141 tgaacagcgt gattatggat ggcgcggtca ttggcgaaga gagcattgtt gccgccatga
    49201 gctttgtcaa agcaggcttt cacggcgaga aacgccagtt gttgatgggt acgccagccc
    49261 gcgctgtacg cagtgttagt gacgacgagt tacactggaa acggttgaat accaaagagt
    49321 atcaggatct tgtcgggcgc tgtcatgcat cgttacatga aacacagcca ctgaggcaaa
    49381 tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa cggtgatctt
    49441 gccggatgcg gcgtaaacgc cttatccaac tctgctggag agcaaccgta ggccggataa
    49501 gatgcgccag catcgcatcc ggcgaccagg tcgcacggta tcatcgaaat gacattaacg
    49561 acgcatactc tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg
    49621 aacggcagga atacgcggtg tctttcttac gccgactaat ttttcccgac tgttattgcg
    49681 caggcgcgcg taaatttgct catcgatatc gaccacttta accagtcgct gacactggca
    49741 tccccgccct tccagcttat tagggatcat cttaacttcg cagcttattt ctgtgacttc
    49801 cgacagaata taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga
    49861 gatctctctt gctatcaccc accgattttc agccatcatc cattcggcga ttaacaagta
    49921 gagtggtttt tcaacatatc cttcacacat acgaactcca gtttcacaaa ttaaaagtct
    49981 ggctatgact cgttttgaag caattaaaca aggccatatt aaaattgtgg atatatcgat
    50041 agtatgcaac ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat
    50101 cgattcccca aaagatttac ttaatgggca aaaaaaacgg tcctcatcag agaaccgtat
    50161 tagttattcg atcaaattag cggatgaaaa atatccgcca tgacacgcta ttatttgatc
    50221 tgtgcgtgca tttcctgcac cgaaattact ttttcagtcg catcggcatt cagcgccatc
    50281 gcggtagcga aaccaccgtt cagggtggtg tcataatgca ctttatattg cagcgcactg
    50341 cgacggatca cgcgggagtc ttcaatcgca cgacggcctg aggtggtgtt gatgatgtag
    50401 gtatattcgc cattcttgat acggtcctga atgtgcggac ggccttcatg caccttgttt
    50461 accagacgcg gattgatacc cgcttcgccc agcacaatcg ccgtgccgtg ggtcgcatcc
    50521 agctcgaagc cctgtttcag cagttttgcc gccaggtcca ccacgcgttc tttatcgcct
    50581 tcgcgcacgg aaagcagcgc acgaccgtgt ttcttcatgg tggagttgct gcccagctgc
    50641 gctttggcaa acgcttcagc gaaggtgcgg cccacgccca tgacttcccc ggtagaacgc
    50701 atttctggcc ctaacagcgg gtcaacgccc gggaatttat tgaacggcag caccacttct
    50761 ttcaccgagt agtacggcgg gataacttct ttggttacgc cctgctcagc cagcgatttg
    50821 ccagccatca cacgcgccgc cacttttgcc agcggtacgc cggtggcttt ggagacgaac
    50881 ggaacggtac gcgccgcacg cgggttaact tcaatcaggt agacttcgtt gtttttcacc
    50941 gcaaactgca cgttcatcag gccgcgcacc tgcaattcga aggccagttt ctgcacctgc
    51001 tggcgcatta catcctgaat ttcctgactt aaggtgtacg ctggcagcga acatgcggag
    51061 tcaccggagt gcacgcccgc ctgctcgatg tgctccatga tgccgccaat cagcaccatt
    51121 tcgccgtcgc agatggcatc cacgtcaact tctactgcgt catcaaggaa atgatccagc
    51181 aacactggcg catcgttaga cacgctaacc gccgtctgga agtagcgacg caggtcagct
    51241 tcgtcataga cgatttccat cgcccgaccg ccgagaacgt aagacggacg caccaccagc
    51301 gggtagccaa tctcttttgc cttctcaact gccatttcaa tagcagtaac ggtggcgttc
    51361 gccggttgtt tcagtttcag acggtcaacc gcatgctgga agcgttcacg gtcttccgca
    51421 cggtcgatag catccgggct ggtgccgata accggtacgc cagcggcttc cagtgcacgc
    51481 gccagtttca gcggggtctg accgccgtac tgaacgataa cgcctttcgg cttctcaata
    51541 cgcacgattt ccagcacatc ttccagagtt accggctcga agtagaggcg atcggaagtg
    51601 tcgtagtcgg tagagacggt ttccgggtta cagttaacca taatggtttc gtaaccgtct
    51661 tcacgcagcg ccagcgaggc gtgtacgcag cagtagtcga attcgatacc ctgaccgata
    51721 cggtttggac cgccgccaag caccatgatt ttttcacggt cggtagacgg attcgcttcg
    51781 cactcttctt cataagtgga gtacatgtaa gcggtgtcgg tggcaaactc tgccgcacag
    51841 gtatccacgc gcttgtagac cgggtgcagg tcatattggt cacgcagctt acggatttcc
    51901 gcttcgcgta cgcccgccag ttttgccaag cgcgcatcgg caaagccttt gcgtttcagc
    51961 tggcgcagga attcagcgtt caggccagtg atgcccactt ccgccacttt ctcttccaga
    52021 cgcaccagtt cttcaatctg taccaggaac cagcggtcaa tgttagtcag gttgaagacg
    52081 ccgtccacag acaggcccgc gcggaaagca tcggcgatgt accagatacg ctctgcgcct
    52141 gcgtctttca gttcgcgacg gattttggtt aacgcttccg ggtcatccag gctcactttc
    52201 gggtcgaatc cagtcgcacc gacttccagg ccgcgcagcg ctttttgcag ggattcctgc
    52261 tgcgtgcgac caatcgccat cacttcgcca accgatttca tctgagtggt cagacggtcg
    52321 ttagcaccgg cgaatttttc gaagttgaag cgaggaattt tggtaaccac gtaatcgatg
    52381 gacggctcga aggaggccgg agtacgtccg ccagtgatgt cgttcatcag ttcatcgagg
    52441 gtgtaaccca ccgccagttt cgccgccact ttagcaatcg ggaaaccggt cgctttcgac
    52501 gccagcgccg aagaacggga cacgcgtggg ttcatttcga taacaatcag gcgaccgttt
    52561 ttcgggttca ccgcaaactg gacattggaa ccaccggttt caacgccgat ttcacgcagc
    52621 accgccatcg aggcgttacg catgatttga tattctttgt cggtcagcgt ttgggctggc
    52681 gcgacagtga tggagtcacc ggtgtggatg cccatcgcat caaagttttc gatagagcag
    52741 acgatgatgc agttgtcgtt tttatcgcgc accacttcca tctcgtactc tttccagccg
    52801 atcagcgact catcaatcag caactctttg gtcggagaga gatccagacc gcgggcgcaa
    52861 atttcttcaa actcttcgcg gttataagcg ataccgccgc cgctaccgcc catggtaaag
    52921 gatgggcgaa taatgcacgg gaagcccacg tcagcggcaa ccgccagcgc ttcttccatc
    52981 gtatgtgcga taccggaacg cgcggtttcc agaccaattt tcttcatcgc tacgtcgaaa
    53041 cgacggcggt cttctgcttt atcaatcgca tcggcagtgg caccaatcat ggtgacgccg
    53101 aactcttcca acacgccctg acgttccagc tccagcgcgc agttcagcgc cgtctgaccg
    53161 cccatggttg gcagcaccgc gtccgggcgc tctttttcaa taatcttacg taccacttcc
    53221 cagtgaatcg gctcgatgta ggtcgcatcg gccatttccg ggtcggtcat gatggtcgcc
    53281 gggttggagt tcaccagaat gacgcggtaa ccctcttcgc gcagggcttt acatgcttgc
    53341 gcgccagagt agtcaaactc acacgcctga ccgataacaa tcgggcccgc acccagaatc
    53401 aggatacttt ttatatctgt acgttttggc atggctcttt tactcctgat tacttagcgg
    53461 ttttacggta ctgctcaatt aactcgataa agtggtcgaa caacggcgcg gcgtcgtgtg
    53521 gaccagggct ggcttcaggg tgaccctgga agctgaacgc cggtttatcg gtgcgatgaa
    53581 tgccctgtaa cgtaccgtcg aacagggatt tatgcgtgac acgcaggttt gcaggtaatg
    53641 ttgcttcatc caccgcaaaa ccgtggttct gggcggtgat catcaccacg tttttctcca
    53701 catctttaac cggatggttg ccgccgtggt gaccaaattt cattttgaca gtcttcgcac
    53761 cgctcgccag cgccagcagc tgatgaccga ggcagatgcc gaagaccgga atatcggttt
    53821 cgaggaattt ctggatggcg gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt
    53881 tggagaggaa gatgccgtct ggattcattt tcagcacatc ttccgcagaa gtttgcgccg
    53941 gaacgatggt caggcgacag cctctatcca ccagcatccg caggatgttg cgtttggcac
    54001 caaaatcata agccacgacg tggaacggca gctcgtcttc ttttttcgct tctggcaggc
    54061 cgccggtcaa cgtccagctc ccttgtgtcc agctataggc ttctgcggtg gtcacttctt
    54121 ttgccagatc catgccattc agacctggga acgcgcgggc tttttctaac gccagcgccg
    54181 catccgggtt atcgcctgcg ataatgcagc cattctgtgc gcctttctcg cgcagtaaac
    54241 gcgtcagctt acgggtatcg atatcggcaa tcgccacgat gttatggcgc ttcaggtaag
    54301 aagagaggtc ttcggtatta cggaagttgc tggcaatcag cggcaggtcg cgaatcacca
    54361 gaccttgtgc atgtacctga gaagattctt catcggcgtc attagtgccg acattgccaa
    54421 tatggggata agtaagagta acgatttgac gagaatagga aggatcagtg aggatttctt
    54481 gataaccggt cattgaagta ttgaaaacga cttccccaac cgccgaacct gttgccccta
    54541 tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa
    54601 caccctccag agaatattca ctcactttat ttgcatatta attcacaatg atgacgtaaa
    54661 tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg
    54721 cgctaaaagt caacttaatg gcaggatttt ttatattatt ttgtggctca tgcatgtttt
    54781 acaggcatta agcgacaaaa agatcatcta acctgttctg gaaaacgctt gcgcagcaag
    54841 aagattacat gaaataaa
//
LOCUS       DAZKIF010000013        53610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZKIF010000013 DAZKIF010000000
VERSION     DAZKIF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.013"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(125..316)
                     /gene="yfgG"
                     /locus_tag="ACHOTW_000843"
     CDS             complement(125..316)
                     /gene="yfgG"
                     /locus_tag="ACHOTW_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP9843005.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            334..486
                     /locus_tag="ACHOTW_000844"
     CDS             334..486
                     /locus_tag="ACHOTW_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843006.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKSSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            668..2911
                     /gene="pdeF"
                     /locus_tag="ACHOTW_000845"
     CDS             668..2911
                     /gene="pdeF"
                     /locus_tag="ACHOTW_000845"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP9843007.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2950..4491)
                     /gene="ppx"
                     /locus_tag="ACHOTW_000846"
     CDS             complement(2950..4491)
                     /gene="ppx"
                     /locus_tag="ACHOTW_000846"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP9843008.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4496..6562)
                     /gene="ppk1"
                     /locus_tag="ACHOTW_000847"
     CDS             complement(4496..6562)
                     /gene="ppk1"
                     /locus_tag="ACHOTW_000847"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP9843009.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6733..7371)
                     /gene="purN"
                     /locus_tag="ACHOTW_000848"
     CDS             complement(6733..7371)
                     /gene="purN"
                     /locus_tag="ACHOTW_000848"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9843010.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7371..8408)
                     /gene="purM"
                     /locus_tag="ACHOTW_000849"
     CDS             complement(7371..8408)
                     /gene="purM"
                     /locus_tag="ACHOTW_000849"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9843011.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8733..9359
                     /gene="upp"
                     /locus_tag="ACHOTW_000850"
     CDS             8733..9359
                     /gene="upp"
                     /locus_tag="ACHOTW_000850"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9843012.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9445..10734
                     /gene="uraA"
                     /locus_tag="ACHOTW_000851"
     CDS             9445..10734
                     /gene="uraA"
                     /locus_tag="ACHOTW_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP9843013.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10829..11530
                     /gene="hda"
                     /locus_tag="ACHOTW_000852"
     CDS             10829..11530
                     /gene="hda"
                     /locus_tag="ACHOTW_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP9843014.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11668..12027)
                     /gene="arsC"
                     /locus_tag="ACHOTW_000853"
     CDS             complement(11668..12027)
                     /gene="arsC"
                     /locus_tag="ACHOTW_000853"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP9843015.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12048..13511)
                     /gene="bepA"
                     /locus_tag="ACHOTW_000854"
     CDS             complement(12048..13511)
                     /gene="bepA"
                     /locus_tag="ACHOTW_000854"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP9843016.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13724..14785
                     /gene="yfgO"
                     /locus_tag="ACHOTW_000855"
     CDS             13724..14785
                     /gene="yfgO"
                     /locus_tag="ACHOTW_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9843017.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14823..15671)
                     /gene="focB"
                     /locus_tag="ACHOTW_000856"
     CDS             complement(14823..15671)
                     /gene="focB"
                     /locus_tag="ACHOTW_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFP9843018.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15693..17705)
                     /gene="hyfR"
                     /locus_tag="ACHOTW_000857"
     CDS             complement(15693..17705)
                     /gene="hyfR"
                     /locus_tag="ACHOTW_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFP9843019.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17735..18148)
                     /gene="hyfJ"
                     /locus_tag="ACHOTW_000858"
     CDS             complement(17735..18148)
                     /gene="hyfJ"
                     /locus_tag="ACHOTW_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFP9843020.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(18141..18899)
                     /gene="hyfI"
                     /locus_tag="ACHOTW_000859"
     CDS             complement(18141..18899)
                     /gene="hyfI"
                     /locus_tag="ACHOTW_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFP9843021.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18896..19441)
                     /gene="hyfH"
                     /locus_tag="ACHOTW_000860"
     CDS             complement(18896..19441)
                     /gene="hyfH"
                     /locus_tag="ACHOTW_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFP9843022.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(19451..21166)
                     /gene="hyfG"
                     /locus_tag="ACHOTW_000861"
     CDS             complement(19451..21166)
                     /gene="hyfG"
                     /locus_tag="ACHOTW_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFP9843023.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(21156..22736)
                     /gene="hyfF"
                     /locus_tag="ACHOTW_000862"
     CDS             complement(21156..22736)
                     /gene="hyfF"
                     /locus_tag="ACHOTW_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFP9843024.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22741..23391)
                     /gene="hyfE"
                     /locus_tag="ACHOTW_000863"
     CDS             complement(22741..23391)
                     /gene="hyfE"
                     /locus_tag="ACHOTW_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFP9843025.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23403..24842)
                     /gene="hyfD"
                     /locus_tag="ACHOTW_000864"
     CDS             complement(23403..24842)
                     /gene="hyfD"
                     /locus_tag="ACHOTW_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFP9843026.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24859..25806)
                     /gene="hyfC"
                     /locus_tag="ACHOTW_000865"
     CDS             complement(24859..25806)
                     /gene="hyfC"
                     /locus_tag="ACHOTW_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFP9843027.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25817..27835)
                     /gene="hyfB"
                     /locus_tag="ACHOTW_000866"
     CDS             complement(25817..27835)
                     /gene="hyfB"
                     /locus_tag="ACHOTW_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFP9843028.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27835..28452)
                     /gene="hyfA"
                     /locus_tag="ACHOTW_000867"
     CDS             complement(27835..28452)
                     /gene="hyfA"
                     /locus_tag="ACHOTW_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFP9843029.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(28705..29175)
                     /gene="bcp"
                     /locus_tag="ACHOTW_000868"
     CDS             complement(28705..29175)
                     /gene="bcp"
                     /locus_tag="ACHOTW_000868"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP9843030.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29175..29747)
                     /gene="gcvR"
                     /locus_tag="ACHOTW_000869"
     CDS             complement(29175..29747)
                     /gene="gcvR"
                     /locus_tag="ACHOTW_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP9843031.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29893..30771
                     /gene="dapA"
                     /locus_tag="ACHOTW_000870"
     CDS             29893..30771
                     /gene="dapA"
                     /locus_tag="ACHOTW_000870"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9843032.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30788..31822
                     /gene="bamC"
                     /locus_tag="ACHOTW_000871"
     CDS             30788..31822
                     /gene="bamC"
                     /locus_tag="ACHOTW_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP9843033.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            32035..32748
                     /gene="purC"
                     /locus_tag="ACHOTW_000872"
     CDS             32035..32748
                     /gene="purC"
                     /locus_tag="ACHOTW_000872"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9843034.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32916..33779
                     /gene="ypfJ"
                     /locus_tag="ACHOTW_000873"
     CDS             32916..33779
                     /gene="ypfJ"
                     /locus_tag="ACHOTW_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP9843035.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33794..35809
                     /gene="tmcA"
                     /locus_tag="ACHOTW_000874"
     CDS             33794..35809
                     /gene="tmcA"
                     /locus_tag="ACHOTW_000874"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP9843036.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35883..36581
                     /gene="ypfH"
                     /locus_tag="ACHOTW_000875"
     CDS             35883..36581
                     /gene="ypfH"
                     /locus_tag="ACHOTW_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9843037.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36662..36862)
                     /gene="ypfN"
                     /locus_tag="ACHOTW_000876"
     CDS             complement(36662..36862)
                     /gene="ypfN"
                     /locus_tag="ACHOTW_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP9843038.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36890..38017)
                     /gene="dapE"
                     /locus_tag="ACHOTW_000877"
     CDS             complement(36890..38017)
                     /gene="dapE"
                     /locus_tag="ACHOTW_000877"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP9843039.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38021..38377)
                     /gene="yffB"
                     /locus_tag="ACHOTW_000878"
     CDS             complement(38021..38377)
                     /gene="yffB"
                     /locus_tag="ACHOTW_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP9843040.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
     gene            38758..38817
                     /gene="ypfM"
                     /locus_tag="ACHOTW_000879"
     CDS             38758..38817
                     /gene="ypfM"
                     /locus_tag="ACHOTW_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP9843041.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(38916..42029)
                     /gene="acrD"
                     /locus_tag="ACHOTW_000880"
     CDS             complement(38916..42029)
                     /gene="acrD"
                     /locus_tag="ACHOTW_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP9843042.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(42193..43893)
                     /gene="narQ"
                     /locus_tag="ACHOTW_000881"
     CDS             complement(42193..43893)
                     /gene="narQ"
                     /locus_tag="ACHOTW_000881"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP9843043.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            44099..46078
                     /gene="aegA"
                     /locus_tag="ACHOTW_000882"
     CDS             44099..46078
                     /gene="aegA"
                     /locus_tag="ACHOTW_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP9843044.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            46146..46721
                     /gene="nudK"
                     /locus_tag="ACHOTW_000883"
     CDS             46146..46721
                     /gene="nudK"
                     /locus_tag="ACHOTW_000883"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP9843045.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            46847..47890
                     /gene="ypfG"
                     /locus_tag="ACHOTW_000884"
     CDS             46847..47890
                     /gene="ypfG"
                     /locus_tag="ACHOTW_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP9843046.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(47985..49988)
                     /gene="tkt"
                     /locus_tag="ACHOTW_000885"
     CDS             complement(47985..49988)
                     /gene="tkt"
                     /locus_tag="ACHOTW_000885"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9843047.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(50008..50958)
                     /gene="tal"
                     /locus_tag="ACHOTW_000886"
     CDS             complement(50008..50958)
                     /gene="tal"
                     /locus_tag="ACHOTW_000886"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9843048.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            51247..53526
                     /gene="maeB"
                     /locus_tag="ACHOTW_000887"
     CDS             51247..53526
                     /gene="maeB"
                     /locus_tag="ACHOTW_000887"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP9843049.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT        13036 a        13859 c        14112 g        12603 t
ORIGIN      
        1 atcagtcggg acaaagacgt ataatttaag atgaaagaat atctgccgct ttgataattc
       61 caaagagcaa aagcatccga cagcgaagcg ccgttatgtt ctgtgaaagt ggagaaggta
      121 accgctaacg ttgtaccggt gattcgacgg agagtgtgga ttgctgagct tcttttttgc
      181 tctgatggtg ctgccaggca ccgacggacg agtagataaa acggccaaag aagaagataa
      241 agctgatgag cagtacgata cgggtcatgc gactgttaaa tcggtgtcgt tttcgcatac
      301 tggttgcctg actcacaaaa ggttccttga agtatgtccc acgccgtgga cggtacttac
      361 attaaggcac aacaggacaa aatggtcaat tcttcgttat gtaaaaaatc gtcagtggat
      421 acatatttta atgttatgga agttaattta attatttaca atgatgatgt aataatgatg
      481 aattaacatg ataaacgtaa taattcatta atcagggtta ttttgtttga tatatatcaa
      541 ttggaacttt catatgacac ttagaatcaa tgctctctat agtgatgaat aatcatcatt
      601 cgaagtcagg tgggatgcct gtctgaatac acctccttca ggatgtgggg gattctgctg
      661 agcatctatg aaactgaatg caacttatat aaaaatacgt gataaatggt gggggcttcc
      721 gctgttcctg ccttctttaa tcttgcccat tttcgcccac attaatactt tcgcgcatat
      781 ttcttccggt gaggtttttc tcttttatct gcctctggca ctgatgatca gcatgatgat
      841 gtttttcagc tgggcggcat tgccagggat cgccttaggg atttttgtcc gcaaatatgc
      901 agagctgggt ttttacgaaa cgctctcatt aacggctaat tttattatca ttatcattct
      961 ctgttggggc ggttacaggg tctttactcc ccggcgtaac aacgtttcac atggtgatac
     1021 ccgtttaatt tcccagcgta tattctggca gattgtgttt cctgcaacgc tgtttctgat
     1081 acttttccag tttgctgcat ttgtaggatt actggcgagc agagaaaatc tggttggcgt
     1141 catgcctttt aatctcggga ccttaatcaa ttatcaggcc ttactggtgg gcaatctgat
     1201 tggtgtcccg ctgtgctact tcatcattcg ggtagtgcga aatccgtttt atttacgtag
     1261 ttattattcg caattaaaac agcaggttga tgtcaaagtc accaaaaaag agttcgcact
     1321 ctggctgctg gcattaggtg ctttactgtt gctgttatgc atgccgttaa atgaaaaaag
     1381 cacaattttt agcaccaatt ataccttgtc attattgctg cccctgatga tgtggggagc
     1441 gatgcgctat ggttataagc tgatttcgct gctctgggcg gtcgtgttga tgatcagcat
     1501 ccacagctat caaaattaca ttcccattta tcctggctat accacgcagc tgaccataac
     1561 ctcctccagt tatctggtat tctcttttat tgtcaattat atggctgtac tggcaacccg
     1621 tcagagaacg gtagtcagac gcattcagcg ccttgcgtat gtggacccgg tggttcatct
     1681 gccaaatgtt cgcgccctga atcgcgcgtt gcgtgatgcc ccctggtctg cgctttgtta
     1741 tttacgcatc cctggcatgg aaatgctggt taaaaactat ggcatcatgc tgcggattca
     1801 atacaagcaa aaactttctc actggctgtc acccttgctg gaaccaggtg aagatgttta
     1861 tcagctttcg ggtaacgatc tcgcgctgcg actgaataca gaatcgcacc aggagcgcat
     1921 taccgcactg gatagccatc tcaagcaatt tcgtttcttt tgggatggca tgccgatgca
     1981 accgcagatt ggcgtcagtt actgctatgt gcgctcgcca gtgaatcata tctacctgct
     2041 gctgggagag ctaaatacgg tcgccgaact ttccatcgtg accaacgccc cggaaaatat
     2101 gcagcgtcgc ggggcaatgt atttgcaacg cgaattgaaa gataaagtcg cgatgatgaa
     2161 tcgactacag caggcgctgg aacacaacca ttttttcctg atggcccagc cgattaccgg
     2221 tatgcgtggt gatgtttacc atgaaattct tctgcgcatg aaaggtgaga atgatgaact
     2281 aatcggcccc gatagcttct tgccggtcgc gcacgaattt ggtttatcgt cgagtatcga
     2341 catgtgggtc attgagcata cgctgcaatt tatggctgaa aacagagcga agatgcccgc
     2401 tcaccgtttt gctattaatc tgtctccaac ctcggtatgt caggctcgtt ttcctgttga
     2461 agtcagtcag ttgctggata aatatcagat tgaagcgtgg caacttattt ttgaagtcac
     2521 cgaaagtaat gctctgacca atgttaagca ggcgcaaatc accttgcagc atcttcagga
     2581 attaggctgc cagattgcga ttgatgattt cggcaccggc tacgccagct atgcgcggct
     2641 taaaaatgtg aatgccgatc tgcttaaaat tgacggcagt tttatccgca atattgtgtc
     2701 aaatagtctg gattatcaga tagtggcatc gatttgccac ctggcgcgaa tgaagaaaat
     2761 gcgggtagtg gcagagtacg ttgaaaacga agagatccgc gaggcggtgc tctctttggg
     2821 gatcgattat atgcagggtt atcttattgg taagccgcaa ccgttaattg atacgctgaa
     2881 tgaaatcgaa cccattcgcg aaagtgcctg aataatgcgg gccgacattt ctcgtcggcc
     2941 cgcaaagcat taagctgcga tttcaggtgt actttcttct tcaattttca accgccagcc
     3001 agccacgcct tcccagtatt cttgctcctt ttccagatca agcagtacca gcgcattctg
     3061 actaaaccag tcatgcggga aacgcagtgt ccagtgactg tcatcagtaa tcagcgtcaa
     3121 tgttggcggt gtggtggttg cctgacgctg attgttgagt aatacgccaa ggcgcaatag
     3181 ctgaatcagt ggcaggaact gtttcttctt aaacaaggta aagcgcggca gatcgtcgag
     3241 tttaatcgct ttacggtgat agcgcaccag tgtcgccatc atcagctgct gttcctgatt
     3301 aaaacccggc aagtcgctgt tttgtagaat ataagcggag tgacgatgca aaccgctgtg
     3361 gttgatattc aacccgacct catgcagcat ggcggcccat cgcagtagcg cctccagttg
     3421 cggatgcgcc agcttcggtt gctgttcccg ccactgttcg tacatttgca tagtggtatc
     3481 cagcacccgt cgggcctgtt cgctgtcgat gtgatactgg ttggcgaggc tgctggcggt
     3541 gcgactacgc acatcctgat gacggaaacg tccttccatt tcatacagta cgccttcgcg
     3601 taacgccccg tcagaaaggc gcagttcacg gatggctaaa gcatcaaaca caccgcataa
     3661 aatcgctagt ccaggaacga agactgtttt ccgctcttcg gaaagacctg gtaaactcag
     3721 cgatgcgaaa ttacggtgcc gtaaaacttc ttttaccagt ttttccagac gttccggggt
     3781 aattatcccg tctttctcgc ccatttccat taacacttca tgggcggctt ttatggtacc
     3841 ggaagcgccc attgccacgt tccagccctg aatacgaaat tgccaggtta aagtttccag
     3901 tttttgtgct gccgccatgc gagcgcgctg aaaattctct ttattgatga ccccgccagg
     3961 gaaataaagc tgggcaaagc tgacacaccc catccggcgg ctttcaacga gaatcggttc
     4021 gaaattttca ccaatcacca gttctgtaga accgccgcca atatcaataa ccagtttgcg
     4081 acctttttcc ggttgggtat gttccacgcc cataaaaatc agacgggctt cttcattacc
     4141 ggaaataatt tcaatcgggt agggaatgac cttttccgca cgtttcagaa agtcagtggc
     4201 gttcagcgcc tgacgcagcg tatgggtacc aactatacag acgctggcag gagaaaaccc
     4261 ttgtagccgt tcggcaaaca gcgacagaca gttcaaaccg cgcgtcattg cctcttcact
     4321 caacatatta tctggcccca ggccgtccgc cagatgcacc cgctgtttca ggcggccaat
     4381 aatctgcatg gcaccatcta ccacacgggc tatgaccatg tgaaaactgt ttgaaccaag
     4441 atcgaccgca gcaaactcct gcggacgagg ggatttatcg tgtattggca tagggttatt
     4501 caggttgttc gagtgatttg atgtagtcat aaatcgccaa ctgcgcccgt actttgcggc
     4561 gattgccgcg gggaacgtag cgattactga gttctttatc gatataacgt gctttgaccg
     4621 tatcgctgaa caatatgtcg atgatgtcca gcacccgctg cttcaggcgc ggatcgagca
     4681 gcggcgtcgc cacttcaata cgataatcaa tattgcgcgt catccagtcg gcggaagaaa
     4741 ggtagacctt tttatcgccg ccattttcaa aaatataaac ccggtcatgt tcaaggtaac
     4801 ggtcaacaat actgatggca cgaatgttgt cgctaatgcc ttccagattg gggatcagcg
     4861 aacacattcc gcgaaccagc agattaaccg gtacgccgga gctggaggcc gcatacagac
     4921 gatcaaccag gcctttatcg acaaggttat ttagcttcag ggtgatacca ctgggcagcc
     4981 cttgctgcgc gttggcgatc tcgcggtcca ccatttcata caacaggcgg cgggagtttt
     5041 gcggcgacac cattaaataa tcaaatgtca ccggacggta tgggttttca ataaagttaa
     5101 atacccgccg tacttcgttg gtgatgcgcg catcggcggt cagcaacgaa tagtcagtat
     5161 aaagacgcgc ggttttttcg ttaaagttcc cggtcccgat gtgtgcataa cgcaccactt
     5221 caccgttttc tttacgtgaa atcagaaaca gtttggcgtg aattttcagc cccggcgcag
     5281 agaagataac gtgcacgcct gcttcggtca ggcgcttcgc ccagtgaatg ttggcttctt
     5341 cgtcaaaacg tgcctgtagc tcaaccacca cggtgacttt cttaccgttg tgtgcggcgt
     5401 ggatcatcga gtcgatgatg cgtgaatctt ttgccacacg gtaaatgttg attttaatcg
     5461 ccagcacgct cggatcgaac gaggcctgac gcagcagttc cagcacatgc tcaaaggtgt
     5521 gataaggata atagagcaac acatcgcgtt cgcgaatggc atcaaaacca ttgcggaact
     5581 gggctttatc aaaccaaata tggcgtaaac gcggcagtgg tttgttcacc agattggctt
     5641 tgccgacatt ggggaaatta ataaagtctt taaaattatg ataacgaccg ccggggacga
     5701 tggagtcgta gcgggaaata gtcagttttt cgcgtaacac ttcaaccagc gcattgggca
     5761 tatcgcgctg ataaacaaaa cgcaccggct cagcagttaa acgctgcttg agactggaag
     5821 acatcaactc catcaggctg gcttccatct catgcactaa atcgtattcg gcatcgcggg
     5881 tcatcttcat tgaataggca ttcagcgcgt cataatcaaa gaagcctttg aaaatatcat
     5941 caaggcagta acgcagaatg ttatccagaa gaatcattgg cttgcgtcga cgcggcgctt
     6001 ctggcggtaa attcacaaag cgcggcactt tatctgatgg gatctccaga agcgcgtaac
     6061 ggatggtatc gccacggata atttccaccg ccagataggt gtaatcatct ttcaggaact
     6121 gcactaagtc agtgtcagga ttgattaaaa tcggcgtaat gtgctgacgc agatactgct
     6181 taaaataatg acgcagccag ttttgttgat tgacggagag ctggcgttca ttaatcagga
     6241 agatctggtt gcgcgccatc tccagcaata gctcgttgta gaggccgtcg aattcctgat
     6301 cggctttcag cacccgggac tgaattttgc ccagtaaatg gcgggaatga gagttggagc
     6361 cttgttcttc gctaataatg atgcgtcgct tcagttcagc gaagcggact ttatagaact
     6421 catcaaggtt attggaatag atccccagga aacgcatcct ttcaatcagc gggttagatt
     6481 tgtccgccgc ttcctgaagc acgcgttcat tgaacgataa ccaactgagc tctttttcga
     6541 tgtatagctt ttcctgaccc attaccactt ttactccgtt ttattcacgg gacactgcct
     6601 ggatattatg gcgagcaata ttggcagatg tccaactatg ccagaaaagg gggaaaaaaa
     6661 ctgctggagc ttgcgattta caggtgaatt gaaaaacgcc agcggcagag ctggcgcttt
     6721 aactacgggg cattactcgt cggcagcgta gccctgcggc ggcagacgtt gaccatccag
     6781 ccacgcggcg ttttcgtgca ttttcagacg accatcggca aaccagctaa tcaccagtgg
     6841 ataaatggcg tgttcctggg tttgcacgcg ggcggtgacg tcatcctccg tatcaccagc
     6901 aaataccggg actttcgcct gtaaaataac cgggccaccg tccagttcat cggtgacgaa
     6961 atgcaccgat gtaccgtgct cttcatcgcc attttccagc gcctgacgat ggggatgtaa
     7021 tccgggatat ttcggcagca gagaagggtg aatgttcagc aaacgcccgg catagtggga
     7081 gacaaatgcc gggctgagaa tgcgcataaa accagccagc acgaccacat cgggtgcgta
     7141 catgtcgatt tcatgaatca actcccggtc ataggcttca cgactgtcaa acgcgctggc
     7201 gatgagcgta tgcgttgcaa tacccgcctg gcgggcgcgt tcaaggccga acgcgtcggc
     7261 cttattgctg aaaactgccc gtacggtgcc tttaattttg ttggttttac aggcgtcaat
     7321 aattgcctgc aaattacttc cgttgccgga aataagcacc acaatattca ttattcgata
     7381 accacgcgtt gttcggaatc agaggctttg atgataccga ttttccacgc gttttcaccg
     7441 ttggcattga gcagggcgag ggctttgtcc acttccggag caggcagggc aataatcatc
     7501 ccgacgccgc agttgaaggt gcgatacatt tcatggcgct caacgttacc agccgtttgc
     7561 agccagttga acacttccgg ccactgccag gaggattcat caatcactgc ctgggtatta
     7621 tctggcaata cgcgcggaat gttttcccaa aagccgccgc cggtcaggtg cgcaatggca
     7681 ttaacatcga ccttttcaat caactccagc actgacttca cgtaaatgcg ggtcggtgcc
     7741 agcagatgat cggctaatgg cttaccatca agttcggtgg tttgcggatc acaaccgctg
     7801 acttcaagaa ttttgcgcac cagcgaatag ccgttcgagt gcggaccgct ggaaccgagt
     7861 gcaatcagca catcgccgtc gctgacttta gagccgtcga tgatttctga tttttctacc
     7921 acgcccacgc agaaacccgc gacatcgtaa tcctcaccgt gatacatccc cggcatttct
     7981 gccgtttcgc cacccaccag tgaacagccc gattgcagac aaccttccgc aatgccgctg
     8041 atcaccgctg aagcggtatc aacatccagt tttccggttg cgtaatagtc gaggaaaaac
     8101 agcggttctg caccttgcac caccaggtca ttaacgcaca tggcgaccag atcaatacca
     8161 atggtgtcgt gacgttttaa gtccattgcc agacgcagct tggtacctac gccgtcagtg
     8221 ccggaaacca gcacgggttc acgatatttt tgcggcaatg cacacagcgc accgaagccg
     8281 cccagaccgc ccatcacttc cggacgacgc gttttcttca ctacgccttt gattcttcca
     8341 accagagcat tacccgcgtc aatatcaaca ccggcatctt tgtagctaag agaggtttta
     8401 tcggtcactg cttgggtccc cacgcgttac ttgcggtaga aaaataaaat tcggcgcaat
     8461 tctaacaggg aaagcaaacg tttgcgagac tgctttacac aacctttttg cacgtctttt
     8521 ccccaggcgc gcggcgaaag aagacttgtg ccagggcaaa ggttagtttt cggatggaat
     8581 aatcttcttt cataaccatt tgaatataaa ataactttat ctcaaaccgt tatcattttg
     8641 actaaagtca acgaaaataa tattgccgcc ttgaagaaag gaggtataat ccgtcgattt
     8701 tttttgtggc tgcccctcaa aggagaaaga gtatgaagat cgtggaagtc aaacacccac
     8761 tcgtcaaaca caagctggga ctgatgcgtg agcaagatat cagcaccaag cgctttcgcg
     8821 aactcgcttc cgaagtgggt agcctgctga cttacgaagc gaccgccgac ctcgaaacgg
     8881 aaaaagtaac tatcgaaggc tggaacggcc cggtagaaat cgaccagatc aaaggtaaga
     8941 aaattaccgt tgtgccaatt ctgcgtgcgg gtctggggat gatggacggt gtgctggaaa
     9001 acgttccgag cgcgcgcatc agcgttgtcg gtatgtaccg taatgaagaa acgctggagc
     9061 cggtaccgta cttccagaaa ctggtttcta acatcgatga gcgtatggcg ctgatcgttg
     9121 acccaatgct ggcaaccggt ggttccgtta ttgcgaccat cgacctgctg aaaaaagcgg
     9181 gctgcagcag catcaaagtt ctggtgctgg tagctgcgcc agaaggtatc gctgcgctgg
     9241 aaaaagcgca cccggacgtc gaactgtata ccgcatcgat tgatcaggga ctgaacgagc
     9301 acggatacat tattccgggc ctcggcgatg ccggtgacaa aatctttggt acgaaataaa
     9361 gaataaaaat aattaaagcc gactttaaga gtcggctttt ttttgagtaa agcgcctata
     9421 acacataata cagaggataa tactatgacg cgccgtgcta tcggggtgag tgaaagaccg
     9481 ccacttttac agacaatccc gcttagtttg caacatttgt tcgccatgtt tggtgcaacc
     9541 gtcctggtgc ccgtcttatt tcatattaac ccggcgactg tactgttatt taacggtatt
     9601 ggaacgctgc tgtatctctt catctgtaaa gggaaaattc cggcttatct tggttccagc
     9661 tttgccttta tttcaccggt attgttactg ttgccgttag ggtatgaagt cgcgctgggc
     9721 ggctttatta tgtgcggcgt gctgttctgc ctggtttctt ttatcgtgaa gaaagcgggg
     9781 accggctggc tggacgtgct gtttccacct gcggcaatgg gcgcaatcgt tgccgtcatc
     9841 ggtctggagc tggcgggcgt agctgccggt atggcgggtt tactcccggc tgaagggcaa
     9901 acgccagact ccaaaaccat catcatctct attaccaccc tggcggtcac ggttttaggt
     9961 tccgtgctgt tccgtggttt cctggcaatt atcccgattt taattggcgt gctggtgggg
    10021 tacgcgctct ctttcgcaat gggaattgtc gataccacgc cgattattaa tgctcactgg
    10081 tttgcgctgc caaccctcta tacgccgcgc ttcgagtggt ttgccattct gactattctg
    10141 ccagcggcgt tagtggttat tgccgaacac gtagggcacc tggtagtaac ggctaatatc
    10201 gtcaaaaaag atctgctgcg cgatccaggt ctgcaccgtt cgatgtttgc taacggcttg
    10261 tcgaccgtga tctccggctt ctttggctcc acgccaaaca ccacctacgg ggaaaacatt
    10321 ggcgtgatgg cgatcacccg tgtttacagt acctgggtta tcggcggggc ggcgattttc
    10381 gctatcctgc tttcctgcgt cggtaaactg gctgccgcta tccagatgat cccattgccg
    10441 gtgatgggcg gcgtttcgct gctgctttat ggtgtcatcg gtgcttccgg tattcgtgtt
    10501 ttgatcgaat cgaaagtgga ttacaacaaa gcacagaacc tgatcctgac ttccgtgatt
    10561 ttgatcatcg gcgtcagtgg cgcgaaggta aacattggcg cggcggagct gaaaggtatg
    10621 gcgctggcga ccatcgtcgg tatcggctta agtttgattt tcaaactgat tagtgtgctg
    10681 cgccctgaag aagtggtgct ggacgcagaa gacgcggata taacagacaa ataatgtacc
    10741 gaccgggcag tgttgctgcc cggttcaaac atcatgggat tctgtggtaa acttctcgcg
    10801 attttgtgaa atcctggttg aggtatctct gaacacaccg gcacagctct ctttgccact
    10861 ttatcttcct gacgacgaaa cctttgcaag tttctggccg ggggataact cctctttact
    10921 ggccgcgctg caaaacgtgc tgcgtcagga acatagcggt tacatctatc tctgggcacg
    10981 cgaaggcgcg gggcgcagcc atctgctgca cgcggcttgc gcggaattgt cgcagcgtgg
    11041 cgatgcggtg ggctatgtcc cgctggataa acgcacctgg tttgttccgg aagtgctcga
    11101 cggtatggag catttgtcgc tggtctgtat cgacaacatt gagtgtattg caggcgatga
    11161 gttgtgggag atggcgattt tcgatctcta caatcgaatt ctggaatcgg gcaaaacacg
    11221 gttgttgatc accggcgatc gtccaccgcg gcagttgaat ctgggattac cggatctcgc
    11281 gtcgcgactc gactgggggc agatctacaa attgcagcca ctttctgatg aagataagtt
    11341 gcaggcgcta cagttacgcg cgcgtttgcg tggttttgaa ctgccggaag atgtggggcg
    11401 tttcttgctg aagcggctcg acagagaaat gcgcacgcta tttatgacgt tggatcagtt
    11461 ggatcgtgcg tcgattaccg cgcaacgtaa gctgaccatt ccgtttgtga aagaaattct
    11521 gaagttgtag ataaggtgtt tattgtcgga tgcggcgcga acgccttatc cgaactacgc
    11581 gaagttaatc catagaattc gtaggccgga taagacgtgg caagcgtcgc atccggcaat
    11641 gtgcttcaac tagcgatgga gcacatctta acccacgatt tccagtacct gctccggtgg
    11701 acgaccaatc cgcgctttgc cattcgcgac cacaatcggg cgctccatca gcttcggatt
    11761 atccaccatt gcctgaatca gcgcctcttc gctaagcgaa ctgtcagcca ggttcagttc
    11821 tttataaaga tcctctttct ggcgcatcaa ttcacgggcg ctattcatcc ccagcatttt
    11881 cagcaaatcg cgcagcgttg ccgcatcggc gggtgtctca aggtagagca ccacttctgg
    11941 ctccacgccg ttttctttca gcaggttcag cgtttcccgg ctctttgaac agcgtgggtt
    12001 atggtagatt tttacctgtt tggtcattgt tcttccttta atgcgaatta catcttggta
    12061 taaggcttaa agcgttcctg cagctggcgc aactggtcga tgcgcgcatc gtaacgcgct
    12121 tgttgcaggc tgcctaattt cacctgcgaa ctggcgctac tcaacagcga aatggcctga
    12181 tcgagtcgtc cggcgagcgc ataaccttct gctcgcgcag ccagctcctg atcgcggtta
    12241 tttagcgcgg cttccgcctg tgccagcaaa tcccagccgt tgctgtcatc tttattatta
    12301 aaggtgtagc gattaagaat attggccgct tcttgtggtt gaccgccttg tagataagcg
    12361 ttcgccaggt tgagctgcaa cacaggattg gtgcgcaaat cgcgggcatt tttcagacga
    12421 ttgatcgcct cattggcttt gttttgccca agatcgatat cagtagccag atcgagatac
    12481 catgcgttgc caggttctgc cgccagtaac ggttgcagcg tttttcgcgc ctcgtcgtat
    12541 ttattggctt ccatcgcctg taaagcacga ccatattgcg ccgctcgttg ctgacgaacg
    12601 tttcctttcg cccattcatc cagcaaatca ctggtgagct ggttacgtcc ggaattatac
    12661 atccccagtg tgcgcgcttt cgccagatag aaatcttccg acgactgcac caccatcggg
    12721 cgcatctgat tagcacggtt gcgggcatct gccagacgac tttccggcaa cgggtgagtc
    12781 agtaaaattt ccggcgggcg cgaggagtaa cgcgcctgat cgagtaattt ttcgaggaag
    12841 gttggcatcg cctgcggatc gaatcccgag cgttgcagca cctgaatacc aatgcggtcc
    12901 gcttcctgtt cattttgctg ggtgaaactg atcatcccct gacgcgttcc cgccagtgta
    12961 ccggtcagcg ccgccatccc cgcctgcgga ctggccatcg ccagtaaaat agaacctaac
    13021 gcgccgaccc aggtcagcgg cgcgttgcgc tgctgatctt ccatcgctcg cgccaggtga
    13081 cgttgggtga cgtgggagat ttcgtgcgcc ataactgaag ccagttgact ttcgttatcg
    13141 gaataacgga acagggcaga gtgcagcacc acgttgccgc caaagaaagc aaaggcgtta
    13201 atttcgtcgt tgttgatcag aaagaaatga aacggtgtct taaccgaatt ggcatgcgaa
    13261 accagacgca tccccagcga attaatatat tgcgttaaca gcgggtcatt aattaacggc
    13321 gcgctgccgc gtagctggcg gacataatag tcgcccatct gcatttcctg accaatggaa
    13381 agcgtgcttc ctgcggaggt tcccatatcc ggcaaggtgt ctgcgctgtc ggcaaatgcc
    13441 ggggctacct gaccaatggt catagcagca atgagggttg caaccaggtt ttttttcaac
    13501 tgcctgaaca taacctctat cctgtatttc tgaagatagt catttgaccg atactgtgaa
    13561 atatcgttcc caccgcgccg cctttgagtg taacggcgaa gattagccca ctcaaatgac
    13621 ttttcagttt cctgaatccg tcataaaaag cgaagtcttt tttgtgacat ttcgatacaa
    13681 ttgcggatcg caatcccgct cttgaggttc agggaaggtc tttatgctcg aaatgttgat
    13741 gcaatggtat cgccgccgtt ttagcgaccc ggaagcgatt gccttgctgg ttattttagt
    13801 tgccggattt ggcattatct ttttctttag tggcctgctt gctccgttgc tggtggctat
    13861 tgtgctggcc tatttgctgg aatggccaac cgtgcgcctg caatctattg gctgctcccg
    13921 ccgctgggcg acgtcgattg tattggtggt tttcgtcggt atattgctac tgatggcgtt
    13981 cgtggtactg cctatcgcct ggcagcaggg catctactta atccgcgata tgccggggat
    14041 gctcaataag ctttctgact ttgccgccac gttgccgcgc cgctatccgg cgttaatgga
    14101 tgcgggcatt attgatgcaa tggccgaaaa tatgcgcagt cggatgctga ccatgggcga
    14161 ttcggtggtg aaaatttccc tcgcctcgct ggtcggtttg ctgaccattg ccgtctatct
    14221 ggtgctggta ccattgatgg tcttcttcct gctgaaagac aaagagcaga tgctgaatgc
    14281 cgttcgccgg gtgctgccgc gtaaccgtgg actggcagga caggtgtgga aggagatgaa
    14341 tcaacaaatc accaactata tccgcggcaa agtgctggag atgatcgtag tagggatcgc
    14401 cacctggctg ggattcttac tcttcgggct gaactattcg ctgctgctgg cggtgctggt
    14461 cggcttctcg gtacttattc cgtacattgg cgcatttgtg gtgaccattc cggtggttgg
    14521 cgtggcgcta ttccagtttg gtgcaggcac tgaattctgg agctgtttcg cggtgtatct
    14581 gatcattcag gcgcttgacg gcaacctgtt agtaccggtg ttgttctccg aagcggttaa
    14641 cctgcatccg ctggtgatta ttttatcggt ggtgatcttc ggtggtttgt ggggattctg
    14701 gggcgtattc ttcgccattc cattggcgac gctgatcaaa gccgtgatcc acgcctggcc
    14761 cgatgggcaa atagcgcaag aataatcttc agcccgacga actgtttcgc cgggctgatt
    14821 tttcaggatt cctgacgtaa ataaatagcc cgataacaaa tactcaccag cactgcaccg
    14881 ccgataatat tacccagcat caccgggagc agattggcgg taataaaatg gctgaccgtc
    14941 agtgcaggaa aattgtctgc gctactgtgc gccagttgcc agaaggaggt aggggcgaaa
    15001 tggcgaatgg caatggcgaa cggaatcaca aacaaattgg cgatacagtg ctcaaagcca
    15061 ctggcgacaa acagggtgat gggcaaaatc atggcgacga ttttgtcgca taacgaacgc
    15121 ccgcaataac tcatccacag cgccaggcaa accatcagat tgcacataat gccgaggctg
    15181 acagattcag taaatgtatg atgcattttg ccctcggcgc agtgtaaaac cgcgactccc
    15241 cacatcgcgt tttcactcat caccagcccg gaaaaccaga ttaacaaact gaaacaggca
    15301 atacctgcca gattaccgca ggccaccaga agtgcgttaa tcagccaagt tcgccaacta
    15361 ataacgcccc gactttttgc catcaccgtc attaccgacg aggtgaacag gctggtgccg
    15421 caaaccgcca gcaaaataaa gccgagtgta aagcaaaggc cgcccaccag atgggttaat
    15481 gcctgtgaag acggggcatc ggcgataacg gaaaggtaaa aagtaaatcc gatcgccata
    15541 aacacgccag cggacatcgc cattaaaaag accgacactt tactgcgggc aattttatgg
    15601 gcggcaatcc gttcagcgat tgccgctttt ctggcgctca actgcaagtc gaaagagagt
    15661 ttgtttctca tgcatactcc tgcaaaagca gattacaaca cctcgcgaac cgagatcccc
    15721 agacgctgca ttcgtgacag cagcgtggtg cgcttcatcc ctaatcgtgt cgccgcgcca
    15781 cggggcccgg caacaatgcc attggtttct cgcaatacct gaacaatgcg ctgacgttct
    15841 tcttcgtcat tctctggcgt cgtcgggtgc agcaactgcg ccattgaact gcgaagcgct
    15901 gaatcttcat ttagcgtcgg cagtaaacgg ctttgtcgga cattaagatg taaattcaga
    15961 ctgttaccgc gagtcagcag taccgctcgc tcaatcacgt tttccagctc gcgcacgttg
    16021 cccggccaat cccacgacat caactggcgt agtgcctcgg tcgggatggc gtcaattgcg
    16081 cgattcatat ggcgcgccat tttttgcgtg aaatgttttg ctaaaagagg gatatcttcc
    16141 ggacggtctc gcagcggcgg caattccagt gggaagacat tcaggcgata aaagagatcg
    16201 ctgcgaaact ggcgatcttc aaccatttgc cacaaatcac ggttggtggc ggcaatgact
    16261 ctgacattca ccgggatcgt tctactcccg ccgagacgct caatttcccg ctcctgcaat
    16321 acgcgcagca atttaggctg aagttctaac ggcagatcgc caatttcatc gagaaacaac
    16381 gtaccgccat cggcaatttc aaaacgacca cgatgggtat taatcgcacc agtaaaggcc
    16441 cctttgtcat gaccgaataa ctcactttcc agaagactgg cggggatggc agcgcagttg
    16501 attttgacca gcggcttgtc gcgtcgcggg ctaagttgat ggatcgctct ggcgatcacc
    16561 tctttgccgg ttccggtttc accacaaatc aacaccgtac tgtcgctctt cgccacaata
    16621 tctacctgct ggagcaggtc ttccattgcc tggctttgat agataatgtc accgacgccc
    16681 agattcgaaa ggagctgctc gctaagctgg tggttttctt gctgcaaact ttcctgcaaa
    16741 tcggtcatgc tacgccaggc atcggcattg ccaacggcaa tagcgatgcg atcggctatg
    16801 tgttgtagta gctggcagtt ttcctcacta aagagagtgg tagaggtatg cgccagcaac
    16861 aatgcacccg gtgtatggtt gccaaaggta agcggtatca ggagcgaaga ttcgcagccg
    16921 ttagatgcaa gtaagtgtaa taacgggtcg cgttgccaga gaaacagatc gtctgcacgg
    16981 tgcgtcaagg tcggttgtcg ggtttgtagc gtttgtgtca gcaatacact gtcgccaggc
    17041 atattgcgtg gcagacacgc acaatgtgag gcagaaagat cgctgcacca caggctgaac
    17101 ttctcgttct ttcgatgatc gcccagtacc atactgactg aagccagacc gaaaaaatga
    17161 tggatctcac gagcgacgtc agcgatcaga tcgtcgagat caagatgtga cagcactgca
    17221 ttagtgatat ccaccagtac ccgatgatga tcgcgatctt tctccagcaa acgcagcgcc
    17281 acctgttgtt cttcatcatt gagccatgat tgcagaaccg gggtgataat gcgtgccagc
    17341 cagttaaaca gtgtgcaatc ctggggagta aaacgactgc cgtgggtgcg aataaagcgt
    17401 agctccgcca ttccactact gccgagaagg acggcgtgac gcgaataatc gaccatctcg
    17461 ccctgatact ctgttgttgc cgtcacatcg ctgccatcat catggtagcg gtaatagaag
    17521 ttgcttttga gaacatgcag ttcaataagt tgtaccgggc tgaacggcgg cggcagcggg
    17581 atgaaggcgc gtaataaaga cgccttgccg tgtttctgcg ctaaccgctc tgcgagcatt
    17641 ccgtttaccg cttcaattgt tattccttgc ggcggggcaa acatcgcctc gtctgacata
    17701 gccatcaaca gtccttatta atgagaaatc cttctcacgc aatttttctg gccatcagat
    17761 agatagccgg ttccagcaca atttcatcca acagatgaat gaattgaatg ctacgcgctt
    17821 gccacacaga cgtcgcgtca tctgccagtg gcgcgaatgc ccgggtcaac agggcggtgt
    17881 ggctggcgtc aataacaatc tcaccaaaag tcatcacccc cagcatctta cgtcgggctt
    17941 gctcctcttc gaccagtgca agccaatctt cgtattccgt cagtgggcag cggaaggtga
    18001 cattcagaca atcaatcacg ccaacgtgat gaccgatagc cagcgagtaa tacattacct
    18061 gggagctgtg ttccggcatc tcgtcgctac tggcgacaaa cttttttcgc agcgtccaga
    18121 aaacaatttc cccgcactct tcagtcatgt aacccccgga gtacgcgaaa gagttgctga
    18181 acgatactat tgagacgtgg atcgtcggca tcgcgcaacc aggtgttaac ccgatttcct
    18241 gtaggatcgt tgcttaaatg gcgcaggagc cgatcgcaaa tttcttgccc ctgacgatag
    18301 cccgccagcc gccgcgcttc tcgctcaatg gcgatacgct gtgatggcgg gatctgcggc
    18361 cacaacggtt gcatagtcac cccggtggga tcgcgataat ccaccgcgtg aatcttctgt
    18421 tgcagcaaac cgagcgccac ggcgaaaccg tgaatggtgg cggccggtgt tggcgggcag
    18481 ccggggatcc aaacatcaat ggggacaatg gtgtcgctgc cgccccagac gctgtagaga
    18541 tcgtggaaaa taccgccgcc gacaccgcac gcgccgtagg aaacacagat tttatgatcg
    18601 ggggcagact catacgcccg aagtgcaggc atacgcatcg cccgggtgac tgcgccagta
    18661 aataacaaaa tatcggcgtg acgcggtgat gaaacaacct taatgccaaa acgttctgcg
    18721 tcgaatacgg gtgtgatggc ggcaaaaatt tcgatttcac aggcgttgca gccgccgcaa
    18781 tcgacgcgat aaacgtaggc tgagcgacgg atatcctgta gcaaatgccg cttcatcttt
    18841 tgcgtttgct cgtccagcgt gatgggctgg ctgacatgtt gtgtaagcac tggactcata
    18901 gctgctcctt agccaccagc ggtacatctg tatcgtcgtt gatcagcgtc gcgcgttgtt
    18961 tgcactccgg gcagacgctc gcttgcgccc gcaacatttc gcggttttgt ggcgcatttt
    19021 gttgctgtgc caacaattca gcagccagtg cgacggtttt ttgcggggca aacgggcgtt
    19081 cgcaacggct gcaacgttgt agatggaacg tcgcgcgggt atagagatcg gctttattgg
    19141 tgacggtcag ttcaaagtta ttggtaagct ggatggctct ggtcgggcac acttcttcac
    19201 aacgtccgca gtaaatacaa cgccccagat agagctgcca ggtgcgcgaa ttttgctggt
    19261 cgtcggtctg gatagtcagc gcatttgtcg gacaagcaca ggcgcaggca ccacaggcaa
    19321 tacattggct gggcatcagg tccggtttcc cgcgaaagcc tgggctgacc tccagtggcg
    19381 cgaagggata tttcaccgtc gcggttccgg cgcgcataat agttttcagt aacttcagca
    19441 tcttctgtcc ttacttcagc ggcgaacggt tacgatcaat gccgtagcgt tcgatctctt
    19501 tatacggcac ggtttttgac tggcgcttgc gcacatcaac cagcgtcaca cggtcggtac
    19561 aggagtagca gggatcaagg ctaccgataa tcagcggtgc gtcagaaacg gtattgccgc
    19621 gcagcatgta acgcaacacc ggccagttgg cgtaggtggc ggcacggcag cgccagcgga
    19681 acaatttttg gttatcaccg agcatgctcc agtgcacgtc ttcaccgcgt ggcgcttcaa
    19741 caaagccgag cgcgaatgcg tgaggtttat agctaaagcc ttcggtcagc agtggggtat
    19801 ccggcatgtt gtcgagagca aattccagca ttgccagcga atcaaacgtc tctttgacac
    19861 ggaccatcac gcgggagaaa acatcgccgc cggtgaaggt aaacagtgtt tttggaatat
    19921 tgccgtagtc ggcgtagggg tgatcaaagc gcaaatcacg ggcaaaacca ctgccgcgga
    19981 tcagcggccc tacagggcta taatcacggg cgatttgtcg gtcgagaatg ccaatgccct
    20041 gagtgcgttg ttccatattc ggcgtggcaa gcagcatttc taccagctcc gacacgtcgg
    20101 cgcgcatctc gcgcaccagt ttcagcgttt gcagacgttg ctctttgaga atatcgcggc
    20161 gaacaccacc aatcagattc agaccgtagg ttttacgcga cccggtcagc aattccgcca
    20221 ttgtcatcga cttttcccgc acgcggaaaa attgcataaa gccggtatca aatccaacaa
    20281 aatggcagga gaggccaagg ttgagcaaat gactgtgtag ccgttcgact tccagcagaa
    20341 tcgagcgaat ggtatgcgct cgttgcggca cctcaatccc cagtgcattt tcaaccgagt
    20401 tggtataggc cacactgtgg gcaaaaccgc aaatcccaca cacgcggtcc gataagaagg
    20461 tcacttcgtt gtagcccatc cgcgtttctg ccagtttctc catgccgcga tggacataaa
    20521 acagacggta atcagcatcg acaatttgct cgccatccac aaacaagcgg aagtgacccg
    20581 gttcatcgga ggtgatatgc agcgggccga cagggatcac ccgcgcaccg ctgttgccct
    20641 cattgataaa cggatacgtt tcggaatcag tcgtcggttc agggcgcagt cgataatcca
    20701 tcgcatcttt gcgcagcgga tgcatatctt ccggccagtc atcgggcaac accaggcgac
    20761 gctgatccgg caggccaacc ggaatcagcc cgtacatatc acgaatttct cgctcgcccc
    20821 agaccgcggc agggacgcgc ggtgtgactg acggaaactc ccgactgtcg gcatcgacca
    20881 gcgccttcac cacaatccag catttttcgg ccccttccat tgaaagggca taataaaccg
    20941 cgtaatgacc gttaagtgtc cgctcgtcgt tgccaaacag gaccggaagc cagccatcat
    21001 gttgataata aagatactgt acaacgtcag gcagcagatt gattttcacc gtaatggtga
    21061 cctgttcagg cgtttgtcgc tcttcatcca gcaccgcgcc ggggaactgc gttttcaggg
    21121 cggcgagaat cgcttcgcca cgatttgacg atgaattaac gttcactgtg tttctccgaa
    21181 acagatgcgg tgcctgaagg caaaaaatca tgccaggtgc tacgttgtgc aggcaggtcg
    21241 tgcgtccctg agaggactat agtggaagcg cccgccagga tcctgatgac aggttgtgga
    21301 atatgcgttc ccatcgccag catcatgacc agcagaatca ccattggcga ggtggtcaac
    21361 cagccgagat caccccggtt aacggcctgc ggcggtttcg ccattaacac ccgcgcagcc
    21421 atccgtacca ggcccgccag caccagcgtt aacagcaata acagcaggac gataatcagc
    21481 aggtgattac gtgccagtcc ggcggtaacg gtcataaatt cgctaagaaa aatgttgaag
    21541 ggcggcatcc ctgccagcgc cagcgcaccg ccgccaaaca gcacggcggt aaatggcatg
    21601 attttgagca tcccacagac gacgttgaga tcgcgcgtgc cgtacttgag cagtacattg
    21661 ccggaaccgc agaacagcag cgtttttgcc agactgtggt ttaaggtgtg cagcagcgcg
    21721 gcaaaaattc ccagcggccc gccaatgcct agcgccaccg cgaccagtcc catgttctcc
    21781 acgctggagt acgccagcag acgcttaatg tcccgctgta ccagaatgaa aaatgccgcc
    21841 acggcaaccg acaacatgcc gaagatgagc aacaaccggt tggggaaatc gctgccgatg
    21901 gcctggcaaa taatgatgta atagcgaatc agcaccaaca gcgcgcagtt cagcaatacg
    21961 gcggagagta gggcgctgac cggactcggc gcttcactgt gagcatccgg cagccaggcg
    22021 tgcatgggaa atagcccggt tttggtacca aagccaatta gcacaaacac aaaggccaac
    22081 agcattaatg ttggatcaag caaggacgat tgcttaagaa cctcgctcca gaatatcgcc
    22141 atttctgcct gcggcataac gctggcggcg ttggcgtata ccagcacggt accgaacaga
    22201 ccaaaagcga caccaacagt acaaataatg atgtacttcc atgctgcttc cagcgatgaa
    22261 cgctgaccgt aaatgcctac cagaaacgcc gagcttaagg tggtggcttc gatcgccgcc
    22321 cacatcacaa tcaggttatt gctggtaaca accagcagca tggtgaacaa aaacagatgg
    22381 aagaaaccgt agtaatcgca cagcgtaacg ggtgaaagct cgccgtgctc aacttcatga
    22441 cgcatgtagc caatcgagta aacaccggtg agaaagccaa tcacaccaag aatggcgagg
    22501 aacaaaccgc ccagaccatc aatatgaagc cacagtcccg cagcgaatat ttctcctgca
    22561 tcagcggcag tttggaccac ccagagtgcc agaatcagca gcagtgtgat ccctaagcta
    22621 tgtaatactg tcaccgtgcg agtcgcagag tgccctcgtt tccggcaggc aaaacagagc
    22681 agcgaaaaaa gcagcggagt gagcaggagt aaagcgaaca tcacagaata actcatctca
    22741 ttatcccttc agcgcggtca agttgttcac gtccagcgtg ccgtgggtac gccatatttt
    22801 tcttgccagt aacaccatca caatgacggc gaagatggcg tcggtagcga taccaatttc
    22861 caccagttcc ggtgcacgcc aggctagaag cgccagcacc agatgggagc cgttttccat
    22921 caggcagtaa ccaaaaattt gccgcaggat attgcgctgg ctaacaatgc acagcaggcc
    22981 aagcagaaaa tgacctaacg ccaccgccag cgctggtttc agcccggtag ccatcggtag
    23041 cttcacgggc tgaacgacaa atgcgcaaag caggacaatt aacgccgcga gcagtgccat
    23101 cattgccgga ccgaataacg ctttttccgg gatgttctgg ggaatatttc gtgcagcgta
    23161 agtcatgatt aacggtacca gcagcacttt ggtgataaag gcgctggcgg accagatcag
    23221 cagttgctct gcggcgaaca ggcacgagag ggtggcgaaa atagacacca gcaccagcga
    23281 ctggcaggcg taaaatccgc atgacaggcg atagcttttg acgctaatca caaacagcga
    23341 tgtcagcatc atcagtcccg ccagattatt tacgatcata gaaccggtca ttcatcatct
    23401 ccttactgca accaggtcgc ggcgattacg ctggaaatca gcgacatcac aatcagtaca
    23461 atcagcacca ggcgcattga ccctggcagc ggtgcggcat cggcgacggc ctcgctcggt
    23521 ttgccgggga cgacgcgacc aaaccagcga ataaaccagg cgaaactggc gaccgattca
    23581 atcatcagca gaatcatggc gggcagcagg atccagtact ccactgacaa cgcaaaaccg
    23641 gcagcaaaca gcgggaattt actgaagaag ccgttgaacg gcggtacgcc ggtaatcgcc
    23701 agcgctgcca cgcagaaacc cacgcctggc aacggcaggg tgtgcaatac gccacgcaga
    23761 cgcggcaaca agcgcgtgcc gcagctgtaa ctcagcgcac ctgctacaag gaaaaacagg
    23821 cttttagcga acgcgtggtt gacgatgtag gcgatgctgc cctccagcgc cagccgcgag
    23881 ccgaagatgg agagcgacaa gccgaagaac atccagccaa gttgagtgat ggtcgaccag
    23941 gccagcaacc gcttcatatc ctgctgtggc aaatacatca gaaatccata aagaatggtg
    24001 accagggcca tgaccatgcc aacgccgcca atcacatgcg ggatattgcc gccgtcgata
    24061 atggcgcggg caaaaatgta aacgcccact ttcaccatcg atgcggcgtg gagataggcg
    24121 ctgatcggtg ttggcgcttc cattgcgtcc ggtagccacg cttgcatcgg tagctgggcc
    24181 gatttccccc acgcggcaaa caggatgccg ccataaacca gataacgtgc gtcgccgtgt
    24241 aactcgctca tcgcgctaag cgcaaacgtt ccggtctgca aaaacagcgt ggcggcggca
    24301 agatacaacc ccaacgaacc gatatgggtg ataagcaacg cttttagtgc tgaacgctgc
    24361 gctttatcgc tctgataata actgatcaac acccaggagc agccgcccgt aatttcaaaa
    24421 aacaacaact gaccgagcag cgtcgaggag agtaccagtc ccgccatcgc gccgataaac
    24481 accagcaaaa atgcgtaata acgattcgtg ccgttatgcg ggtgttcgcg atttttatcc
    24541 gtcagataac ccgtggagta gatcgtgacc agcaaaccga gaaacaccac cacaaacaga
    24601 atcagcgtac acacgcgatc aatgaccagg ccaaacaatg ccacatcccc cacgttgacc
    24661 aacgtcaacg tgacggcaac tttatcggcc tgataaaacg cggagatcag cgacaacatg
    24721 cacagcgtgg tcagcgcggc gaacaaaacc ccccattcgg cggcccgacg ttgtggcgaa
    24781 aacgaaacga ccagtgcgcc gataaaaggc agcaataacg tcgtcagagc aagattttcc
    24841 atattccgtc agtgctcctt acagaccggt taaccagaag acccatgcaa gcgcagcaag
    24901 gctgaagcca agccaggtca catggtgaat gagtaaaaaa cgcccgcgtg ccagcgtgtt
    24961 ttctgcgatt gaggccagta caaaaatcag caaaaccttg agcagcgtaa cgacaagtga
    25021 agtcagcagg caggcgagag aaagttcttg cgcgcaccca aagggcagaa acagggccac
    25081 aaacagtgct gccatcacga cctgtttcag ccccagcccc catttcgcca gcgccagccc
    25141 ggcaccggaa tattctgtca gcgggccttc ctgtaattcc tgttctgctt cagcaacatc
    25201 aaagggaatt tttcccatct caatgaagca ggcaaaacca caggccagta acgccagtac
    25261 ggtggttagc ggcgagttcc agcccgttgc cagcgtattg ctgatcatct cgatatgcgt
    25321 ggaacctgct atcagcgcca ataccagcag tgagagaatg agcattggtt cgaccagaat
    25381 gccgagcgtc aactcgcgac tggcaccgac tcccgcaaac gggcttccgg tatccagccc
    25441 ggaaagagca aagaaaaaac gaaacagggc aagaagatag ataagggtga tcagatcgcc
    25501 gccgcccgca aaaggggaaa cggtaataaa tagtggtagg gccatcgcca gcaccagcat
    25561 gctgctgatt aatacccacg gcatcaggcg gaacatcaga cctgaagata taggcgcaac
    25621 ttcctggcgt ttaaacagtt tgtggatatc gcgataatcc tgccagatcc ccggcccgcg
    25681 gcgggagtgc atacgcgcgc gtatctgccg ggaaataccc gtaaaaagcg gggttagcat
    25741 cagcagaatc acggcctgtg ctaacgcaaa aatgaccaga tatccgtcgc aaagagtttg
    25801 tctcatggtg atttccttag acggcaatag cgattagcag cacaaccagt gcggcgacca
    25861 catacaggca atagagacga aagtcgccgc tttgcagatg ctgtatttct ttggccagcc
    25921 gttgggtggc gctcacgatg gggcggatca cccgctcatc ccagaagggt tctgtgctct
    25981 gagccctggc ggtgacgtgc gcaagacctt tatttagcct cagcgtaggg tcgagctgtt
    26041 gtcgtagacg aaatagcgca gagaagacca cacgcagcgg ctgcatcacg ccattgcctg
    26101 acggggccat cgcattttcc cagccgtagc cgcatgccca ggcatctcct gtgcgacgaa
    26161 acgctgcgcg gcgcgaacga caccacagcc agtaaaggcc aggcagcaaa ggcatcgcca
    26221 gtagtaacag caacagtaat gagggggtga cctgtgtatg aaacgtgccg ggtacaagtg
    26281 ctattccgct ggcgacagtg acgggagggg tattggtaaa cgcatgggca atatgcatta
    26341 tcttcggtgc cagccaactg gcactaagcg caattaatac gcagagtgcc gcgagcagta
    26401 gcatggcgac gatcatcgta tttggcactt cctgtgcctc ttcagcgtgt gtactgcgcg
    26461 gcgcaccaca gaaagtaata ccgtacattt tgacgaagca cattactgcc agcccaccag
    26521 tgactgccag catcaccata gcaataggac ccgcaagttg tagcgctacg gcttccacac
    26581 gacttagtga gaacagcgac tgccaggtgt accattcgct gataaaacca ttcagcggag
    26641 gaagggctga tatcgcgagg caaccaatca ggcatgctgc ggctgtccac ggcatccgtt
    26701 tcgccagtgc ccccattttt tccatgtcgt gggtatgcaa acgcgaaata atcgcgcccg
    26761 cgccgagaaa tagcagccct ttgaacagcg catggttgag cagatgaaac agtgcgccga
    26821 gcagtccaac cacggtgagc agcgggtcgt gtagtgacag accgaccatc gccacaccga
    26881 ctgccagcaa aataatgccg acgttttcga cggtactcca ggccagcagc cgtttgatat
    26941 cctgttccgc cagcgcatac agcacgccca ggagcgcgga gattgcgccg atcgccatca
    27001 ccagaatgcc ccaccacagc ggcaaacccg tttgcgccag cagatccatc gccactttca
    27061 ggatgccgaa aataccgatt ttgaccatta cgccagacat caacgccgaa gcgtgcgatg
    27121 gtgcggcagg gtgagcgcgc ggcaaccagc tgtgcaacgg catcatcccg gctttcgcgc
    27181 caaaaccgaa aaaggccagc aggaacaccg ccgacgccag ccccggagaa agtgaaagcg
    27241 tgcggaaact ggcaaaatct aggctgccgc tttcgcgccc catcagcaag aaggcgatca
    27301 taatcagcac cgatccggcg tgggcgataa aaaagtagag catgccagcg ttgatcgacg
    27361 ttttatcctg cctggcaatg accagaaacc aggaagacag cgacatcatt tcaaacagca
    27421 cgatgaacca aaaagcgttg tccatcacca gcagggcaac catcgatgcg atgaaaatat
    27481 tcataaagaa gcccatcgcc gccgcgcctt tgccctcgta ttcgcgcata taagtcaatg
    27541 aatagagcga acaaaccacc accagcaagg agataaccag caccataaat gccgagaggc
    27601 tatccattcg cagggttaaa tcggcaaagt caaaaggggt ggcaaaacgg gcaactaaag
    27661 ttacgccttc atgtaattgc gtaattccgc tgatgatgcc aatcacgccg cccaccagcg
    27721 atgtgatgcc ggaaagctta atagccagtc tgtccagacc gagtaaaaac agcgaagcca
    27781 gactggcaaa cagatagagg atcagcgacc aggttaataa ttgcagggca tccattagcg
    27841 ttgctcctga gtgagggaaa ggggatcctc cctgccattg gcaaaccagc ttgcggcaag
    27901 gcgctgtttt tctttcatct gacgttgcag gctatcgtcg gtgatcagcc gtaacgcctg
    27961 attcgggcaa gcgcgaacgc aggcaggacc ttctggcaag aaatcacaca ggtcgcattt
    28021 caccgcgacg gtctggacac ccggttccca gcgcagcaaa gcatgttgtg ttggcgcgtt
    28081 ttcttcgccg tctttgagtg agccttcagc ctgaaaaaca tattgcgcag gggcattcac
    28141 cggacggctt cctgaagcgc tgattgcgcc aaatgggcag accacggcgc aaagcttgca
    28201 gccaatacag aggctttcgt tgagttggat cgcatcatcc ctctgggaga tggcattgac
    28261 cgggcagacc tgcacgcaag gggcttcctc acagtgatga cacacgacag gggcagtgag
    28321 tgttgacgtc ttcgccaggg ccaggcgcgg gtgttgctgt aaaccttgcg ttttatgcac
    28381 gtccgaacag gcagcgagac aggtattaca tcctgtacac cacagtggtt cggccaccac
    28441 aaagcggttc atgtcggctc cttgaaaatg atatgaaaca ttgcatttca taatgcataa
    28501 aggatgtcag tatgattaat ttaatatatt caataagtta tgaattaact gaattctgaa
    28561 cggtacaaaa atgatggtca ttaacgttca gcaaaaaatg gattcgacac ccgtgtcgca
    28621 ggggaaagtg agaatgtgat cttgatcacg ataagtgagg tgtgctgacc gcaagcgcag
    28681 ccagcacgga atggagcaaa gtaatcaggc gtgttctttc agccagttca gcacaacgtc
    28741 gtggtgattg ctggttttga aatcgtcaaa gacatgttcg attttgccat cagcgtcaat
    28801 caggaagctg atgcgatgaa taccatcgta ggttttgccc atgaaggact tttcccccca
    28861 gacgccgaat tgctcgcaca cctggtggtc ctcatcagac aggagcgtaa agttaagcag
    28921 ctctttttcc gcaaaacggg agagtttttc gggtttatcg gtgctgatac ccagcacatc
    28981 aacgcccgct tttttcaact catccatgtt atcgcgtaag ccgcaggcct gtacggtaca
    29041 gccgggggtc atggctttcg ggtagaaata aaccagaaca cgctgtccct ggaagtcggt
    29101 caaattaact tgttctccgt cttgatccgg caagctaaat ttcggtgcga tatcaccggc
    29161 tttcagtgga ttcattactt aactccatcc tgttcatcat gttgggaata attgacgacg
    29221 ttaatactgc cttgtgcatt gagttctgta catagggctt tgaacgcttg ctcaatattt
    29281 gctgcgtccg cagatgcggg gctgtgggca gtaatctgaa tatgcaactg cgcagcccgt
    29341 tcattttcag caggttgtgt gcgcgacacc agctccgcaa tgttcatatg atgcgcgtcg
    29401 aaaagtgctg tgaagcgttc aattaaatgc ggggagtctg ccacatcgac ctgaacccag
    29461 acagatgctg gcattggcgg acgcggacgc gccgtcgtgc gcttcatcac gattaaaaga
    29521 tccagttcgg cacctttcaa cggtaacgtt gattcaatca gagtaatggc attccatgaa
    29581 ccggaaagca gcatgataaa cgtgaactct tctcccagca tcgccaggcg actgtcttca
    29641 atattacagc cgcaactact gacatgacgg gtgatggtgt tcacaattcc agggcgatcg
    29701 gcacccaacg cagtgatcac cagataatgt tgcgatgaca gtgtcaaact ggttattcct
    29761 ttaaggggtg agttgttctt aaggaaagca taaaaaaaac atgcatacaa caatcagaac
    29821 ggttctgtct gcttgctttt aatgccatac caaacgtacc attgagacac ttgtttgcac
    29881 agaggatggc ccatgttcac gggaagtatt gtcgcgattg ttactccgat ggatgaaaaa
    29941 ggtaatgtct gtcgggctag cttgaaaaaa ctgattgatt atcatgtcgc cagcggtact
    30001 tcggcgatcg tttctgttgg caccactggc gagtccgcta ccttaaatca tgacgaacat
    30061 gctgatgtgg tgatgatgac gctggagctg gctgacgggc gcattccggt gattgccggg
    30121 accggtgcta acgctactgc ggaagccatt agcctgacgc agcgcttcaa tgacagtggt
    30181 atcgtcggct gcctgacggt aaccccttac tacaatcgtc cgtcgcaaga aggtttgtat
    30241 cagcatttca aagccatcgc tgagcatact gacctgccgc aaattctgta taatgtgccg
    30301 tcccgtactg gctgcgatct gctcccggaa acggtgggcc gtctggcgaa agtaaaaaat
    30361 attatcggaa tcaaagaggc aacagggaac ttaacgcgtg taaaccagat caaagagctg
    30421 gtttcagatg attttgttct gctgagcggc gatgatgcga gcgcgctgga cttcatgcaa
    30481 ttaggcggtc atggggttat ttccgttacg gctaacgtcg cagcgcgtga tatggcccag
    30541 atgtgcaaac tggcagcaga agggcatttt gccgaggcac gcgttattaa tcagcgtctg
    30601 atgccattac acaacaaact atttgtcgaa cccaatccaa tcccggtgaa atgggcatgt
    30661 aaggaactgg gtcttgtggc gaccgatacg ctgcgcctgc caatgacacc aatcaccgac
    30721 agtggtcgtg agacggtcag agcggcgctt aagcatgccg gtttgctgta aagtttaggg
    30781 agatttgatg gcttactctg ttcataagtc gcgcctggca acggttgcgg gtgtttcgct
    30841 tgttttatta ctcgctgcct gtagttctga ctcacgctat aagcgtcagg tcagtggtga
    30901 tgaagcctac ctggaagcgg caccgcttgc ggagcttcat gccccggctg gaatgatttt
    30961 gccggtgacc tccggtgatt atgcaatccc ggtgaccaac ggtagtggtg ctgtcggtaa
    31021 ggcgctggac attcgtccac cagcccagcc gctggcactg gtttctggcg cgcgtaccca
    31081 gttcacgggc gataccgctt cattgctggt ggaaaatggt cgtggcaata ctctgtggcc
    31141 gcaggtggtt agcgtgctgc aggcgaaaaa ctacaccatc acccaacgtg atgatgctgg
    31201 tcagacactg accaccgatt gggtacaatg gaaccgtctg gacgaagacg agcagtatcg
    31261 tggtcgttat caaatctctg ttaagccgca gggttatcag caggcggtta cggttaaact
    31321 gctgaacctg gaacaggcgg gcaaaccggt tgcagacgcg gcttccatgc agcgttacag
    31381 cacggagatg atgaacgtta tttccgctgg tctggataaa tctgccactg acgccgcgaa
    31441 cgctgcgcaa aatcgtgcct ccaccactat ggacgtacaa agtgcagctg atgacaccgg
    31501 tttaccaatg ctggtcgtac gcgggccgtt caatgtggtt tggcaacgtc tgccagcggc
    31561 gctggaaaaa gtgggcatga aagtgaccga cagcacccgt tcgcagggca acatggccgt
    31621 aacttataag ccgctgtctg acagcgactg gcaggaactg ggcgcgagcg atccaggcct
    31681 ggcatctggt gactataaac tgcaggttgg cgatttagat aaccgcagca gcctacagtt
    31741 catcgatccg aaaggtcata ctctgactca gagtcagaac gacgcgctgg tagctgtctt
    31801 ccaggctgcg tttagcaagt aaaaatacag ggctggaatc atccggccct tttttctgat
    31861 atgatacgca aacgtgtgcg tcggcaggaa aacgcgattt tagcggtaat tcgcacgaaa
    31921 tttgtttgtc ggacgtagtt cggataaggc gttcacgccg catccgacaa aacatccggc
    31981 acaccagaca gcaaaagatt ttaaaacgtt aattcacacc caggagtgat aaagatgcaa
    32041 aagcaagctg agttgtatcg tggtaaagcg aaaaccgtat acagcacgga aaacccggac
    32101 ctgttggtgc tcgaattccg caatgatacg tcagcagggg atggcgcgcg cattgagcag
    32161 tttgatcgca aaggtatggt gaacaacaag ttcaactact tcattatgag caaactggct
    32221 gaagcgggta tcccgactca aatggagcgt ctgctctccg ataccgaatg tctggtgaaa
    32281 aagctggata tggtgccggt tgagtgtgtc gtgcgtaacc gtgctgctgg ctctctggtg
    32341 aaacgtcttg gaatcgaaga aggtattgag ctgaacccgc cgctgttcga tctgttcctg
    32401 aaaaacgacg ccatgcacga tccgatggtc aacgaatctt actgcgaaac ctttggctgg
    32461 gtaagcaaag agaacctggc acgcatgaaa gagctgacct acaaagcgaa cgacgtgctg
    32521 aaaaaactgt tcgatgatgc tggtctgatt ctggtcgact tcaagctgga gtttggtctg
    32581 tacaaaggtg aagtggtact gggcgatgag ttctccccgg acggtagccg cctgtgggac
    32641 aaagaaacgc tggagaaaat ggacaaagac cgtttccgcc agagcctcgg tggcctgatc
    32701 gaggcctatg aagccgtcgc ccgccgcctg ggtgtacagc tggactgatt tttctgttca
    32761 tcatcttgcc gtgctgctgg cacggcaaga caaccattct cgtaagatgt gcattgaacg
    32821 ctcattcctc cgccatttca tcccgttttc ctgtggtaat cttcctctga accacctacg
    32881 atcatttcta taatgaatat attgttgagg tatctatgcg ttggcaaggg cgacgtgaaa
    32941 gtgacaatgt tgaagacagg cgcaatagct ccggcggtcc gtcaatgggc ggtcccggtt
    33001 tccgtctgcc gagcggtaaa ggcgggctga ttttactgat agtcgtgctg gttgcaggct
    33061 actatggcgt tgatttaacc gggttgatga ccgggcagcc ggtttctcaa cagcaatcaa
    33121 cgcggtcaat tagcccaaat gaagacgaag ccgcaaaatt cacctcggtg attctggcaa
    33181 ccacggaaga cacctgggga caacagttcg agaagatggg taagacctat cagcaaccga
    33241 agctggtcat gtaccgtgga atgacgcgta ccggatgcgg ggcgggccag tccataatgg
    33301 ggccgttcta ttgcccggcg gatggtacgg tttatatcga tctctccttc tatgatgaca
    33361 tgaaagacaa acttggcgcg gatggcgatt ttgcccaggg gtacgttatc gcccatgaag
    33421 tcggtcatca tgtgcagaaa ctgttaggca tcgagccgaa agttcgtcaa ctgcaacaaa
    33481 acgcgacgca ggcggaagtg aaccgcttat ctgtgcgtat ggaactccag gccgactgtt
    33541 ttgccggtgt ctgggggcat agcatgcagc agcaaggcgt tctggaaacc ggcgatctgg
    33601 aagaggcgct gaacgcggcg caggccatcg gcgatgaccg tttacaacag caaagtcagg
    33661 ggcgagtagt accagacagt ttcactcatg gcacttctca gcaacgctac agctggttta
    33721 aacgtggttt cgacagcggc gatccggcac aatgcaatac ttttggtaaa agcatttaac
    33781 ttccggggca gggatggctg aactgactgc gcttcacaca ttaacagcgc aaatgaaacg
    33841 tgaagggatc cgccgcttgc tggtgttgag cggggaagag ggttggtgtt ttgatcatgc
    33901 gcttaagtta cgtgatgcct tacctggcga ctggctgtgg atttcgccgc agccagatgc
    33961 tgaaaaccac tgttctccct cggcactaca aactttactt gggcgcgagt tccggcatgc
    34021 ggtattcgac gcccgccacg gctttgatgc cgctgccttt gccgcactta gcggaacgtt
    34081 gaaagcggga agctggctgg ttttgttact ccctgtatgg gaagagtggg aaaaccaacc
    34141 tgatgccgac tcgctgcgct ggagtgattg ccctgaccct attgcgacgc cgcattttgt
    34201 ccagcatttc aaacgcgtac ttacggcgga taacgacgct atcttctggc ggcaaaacca
    34261 gccgttctcg ttggcgcatt ttactccccg tactgactgg caccccgcga ccggcgcacc
    34321 acaaccagaa caacagcaaa tcttacagca gctactgacc atgccgtcgg gcgtggcagc
    34381 ggtaactgct gcgcgtgggc gcggtaaatc ggcgctggca gggcaactca tttctcgtat
    34441 tgcgggtagt gcgattatca ctgcgcccgc aaaagcggca acggatgtac tggcacaatt
    34501 tgcgggcgag aagtttcgct ttattgcgcc tgatgccttg ttagccagcg atgagcaagc
    34561 cgactggctg gtggtcgatg aagccgcagc catacctgcg ccgttgttgc atcaactggt
    34621 atcgcgtttt cctcgaacgt tgttaaccac tacggtgcag ggctacgaag gtaccggacg
    34681 tggttttttg ctgaaatttt gcgctcgctt tccgcattta caccgttttg aactgcaaca
    34741 gccgatccgc tgggcacagg gatgcccgct ggaaaaaatg gttagtgagg cactggtttt
    34801 taacgatgaa aacttcaccc atacaccaca aggcaatatc gtcatttccg catttgaaca
    34861 aacgttatgg cgaagcgagc cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc
    34921 gcactaccgg acttcgccgc tggatttacg ccgcatgatg gatgcaccag ggcaacattt
    34981 tttacaggcg gctggcgaaa acgagattgc cggagcgctg tggctggtgg atgagggggg
    35041 attatctcaa gaactcagtc aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct
    35101 ggtggcccag tcgctggcgg cgcacggcag caatccactg gcggcgacat tgcgtggacg
    35161 gcgggtcagc cggatagcag ttcatccggc gcgtcagcgc gaaggcgttg ggcaacagct
    35221 cattgccagc gctttgcaat ataggcctgg cctcgactat ctttcggtga gttttggtta
    35281 caccggggag ttatggcgtt tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa
    35341 tcatcgtgaa gccagcagcg gttgctatac ggcgatggcg ctgttaccga tgagtgatgc
    35401 gggtaaacag ctggctgaac gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc
    35461 gcagtggaat ggcgaaacac tccctgttga tccactaaac aatgccgtcc tttctgacga
    35521 cgactggctt gaactggccg gttttgcttt cgctcatcgt ccgctattaa catcgttagg
    35581 ttgcttattg cgtctgctac aaaccagtga actggcatta ccggcgctgc gtgggcgttt
    35641 acagaaaaac gtcagcgacg cgcagttatg taccacactt aaactttcag gccgcaagat
    35701 gttactggtc cgtcagcggg aagaggccgc acaggcgctg ttcgcactta atgatgttcg
    35761 cactgagcgt ctgcgcgatc gcataacgca atggcaattt tttcactgac tccttcaatt
    35821 ttctcccatg gtcattgtga gtcagcggcg taaaattaat gacatcagtt aaggagttcg
    35881 ccatgaaaca tgaccatttt gttgttcaaa gcccggataa acctgcgcaa cagttgttac
    35941 tgcttttcca cggtgtcggg gataacccgg tggcaatggg ggaaataggt agctggtttg
    36001 caccgttgtt cccggatgcg ctggtggtga gtgtcggtgg cgcggagccg agcggtaatc
    36061 cggcggggcg tcagtggttt tcggtgcagg gtattacgga agataatcgc caggcgcgtg
    36121 tggatgccat catgccgacg tttattgaaa cggtgcgcta ctggcagaaa cagagcggag
    36181 taggggctaa cgccacggcg ctaatcggtt tctctcaagg cgcgattatg gcgctggaga
    36241 gcattaaagc cgaaccgggc cttgcttcac gcgtcattgc ttttaacgga cgctatgcga
    36301 gcctgccgga aaccgcgtct acagccacga caattcactt gattcatggc ggtgaagatc
    36361 cggttatcga tctggcgcat gcagttgcgg cgcaggaagc gttaatcagt gccggtggtg
    36421 acgtgacgct ggatatcgtg gaggatttag gccatgcgat cgacaatcgc agtatgcaat
    36481 ttgccctgga tcacttgcgt tataccattc cgaaacatta tttcgacgaa gcgctaagcg
    36541 gcggtaagcc tggcgatgat gatgtgattg aaatgatgta ataatagccg ggtaggcctg
    36601 ataagaccgt atcaggcatc atggccggat gcggcgttaa cgccttatcc ggccacttag
    36661 cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac ttatcgttga
    36721 aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttatgg tcgacgcgct
    36781 tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc agaatccacc
    36841 aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat cacgcgacga
    36901 gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca gcgttcacac
    36961 attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc tgcgccccca
    37021 tgcgggcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc ggtttaattt
    37081 cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc gcggtcaaaa
    37141 atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt tcaagcaggg
    37201 caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg aagttaaact
    37261 gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga atattggcaa
    37321 tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata gccactaatt
    37381 cattaaggaa aggtgctgcg cgatgtaccg gattgtcagc cagatgtggg taggcaacat
    37441 gcccctgaac gccatgaatg gttaggttgc aggttaacga tccacgacga ccatttttca
    37501 ccacatcacc taccacttcg atactcgacg gttcgccgac caggcagtaa tcgagacgct
    37561 cattacgtgc cattaacgct tcgacggctt ttaccgtacc attgtgggca ctggcttctt
    37621 catcagaggt gatcagaaat gccagtcgcc ctgtatggtt gggatgttgt gcgacaaaac
    37681 gttctgcagc taccaccatc gccgccagcg agcctttcat atctgccgca ccgcgcccga
    37741 ataacatgcc gtcacgaatg gttggttcaa acggcggatt gatccaacga tcggcgtcgc
    37801 caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc ccacgccatg
    37861 cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg atcgcctgca
    37921 aacgttcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa gggcggcgaa
    37981 taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca tggaaaaatt
    38041 tctgataact ggaatcactg aaacccagca gcataggctt accaggcgcg cagagcaatg
    38101 gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgcagaggcc gcatcggtga
    38161 ttatattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg ttgagtaacg
    38221 cttcccagcc taattcgttg ataaaaccgt tcaataattc gctgtccagc ccatcgacgc
    38281 ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga gcctttttaa
    38341 tggtgtcaca atttttgatg ccgtaaagtg taaccataat gaatcctttt tcgatttatg
    38401 agaataaata ctcatttaag agtgaaaaca ttttgcctgc aaatgattat caggaaatat
    38461 aacattatcg aagtattcaa attaacagct attaaataat attaaacatt tttaacgtaa
    38521 acattaagaa tattcatctt gttatctgtc tgtattttaa ccagaattaa atcagtatta
    38581 gatgccgtat gaatatctcg aaatgttgct gtgtatcaca tacaaatcat cactgcagga
    38641 ccatagtatt tacataaggt ttgtcccccg gaggtagtca tcgcagcaat cgagtaaatt
    38701 agcttcacca cggtatattg tttccgtaga atacccataa ttataagaga ggttgttatg
    38761 attgaacgtg aactggggaa ctggaaagac tttattgaag ttatgcttcg taagtaattc
    38821 ctgagacaaa gtacaaacag caagaacccg ctgagcaggt tcttaatcgg aaagttgaaa
    38881 aaggcgacac attggcatgt cgcctttttt attgcttatt ccgggcgcgg cttcagcggg
    38941 aagcggcggc gcaccagcac aaagaacagc ggcacgaagt aaatagccag aatagtggct
    39001 gaaatcatcc cgcccattac gccagtaccc accgcatgct gaccaccgga accggcaccc
    39061 gtgctggttg ccattggcaa tacgccgaag ataaatgcca gcgaggtcat cagaatcggg
    39121 cgtaaacgct gacggcaggc gtggagcgtc gcttcaaaca ggtcgtggcc tttttggttc
    39181 atctcgttag caaactcgac gatcaggatg gcgtttttcg ccgataaacc aatgaccgtt
    39241 aacaggccca cctggaagta aacgtcgttt tccagcccgc gcatccaggt tgccagcagc
    39301 gcgccgatta cccccagcgg cacgaccagc attaccgaga acggtaccga ccagctttca
    39361 tacaatgcag ccagacacag gaataccacc agcaaggaaa tggcgtacag cgccggagcc
    39421 tgcgcgccgg aaagccgctc ctgatacgac atcgccgtcc actccagacc aaagccgttt
    39481 ggtagctgct tcactaacga ttccataata tccatcgccg taccggtact gacccccggt
    39541 gcggcttccc caacaatttc aaccgcagaa taaccgttat agcgttccag acgcggcgag
    39601 cctgtctccc agcgtgaggt cgcgaaagca gagaagggca ccatgccgcc atctttattt
    39661 cggacatacc agagattgat gtcatctggc agcatgcgat acggcgcagc tgcctgcaca
    39721 tagactttct tcacgcgacc gcgatccata aagtcattca catagctcga accccaggcg
    39781 gtttgcagtg tgtcgttaat atcgtcgata gcaacgccca gcgcctgagc tttacgctgg
    39841 tcgatatcaa tctgcaactg cggactgtca tcgaggccgt tatggcgcac acgggttagc
    39901 tccgggtttt ccgccgccag cgccagcaac tggttacgtg ctgccatcag cgcatcgtga
    39961 cctgctccag cgtggtcctg caactccata tcaaaacctg cagaactacc aagaccgcta
    40021 attgctggcg ggctgctggc gataacgcga gcttctttaa tttggttaaa cgcctttgtt
    40081 gcacgctcga taatggcaaa cgaggtgccg gtcttactgt cgcgttcgct ccagtctttc
    40141 aggcggataa acattcgcgc cacgttttgc ccgttacccc cagggccaga accaacagtg
    40201 gcaaacaccg acatgatgtt gtctttttca tgggtgaagt aatatttctc gatttgctca
    40261 acgactttca gggtctgttg ttgggttgaa ccgctgggca attgtaccga ggtagtaaac
    40321 atgccacggt cttccagcgg taagaacgac gtcggcaaac gcaggaacag gaacaccatg
    40381 ccgccaagca gcaggacata aatcacaatc cagcgcaggc tacggtggag aattttcgcc
    40441 accccttttt cgtagcgttc ggcgttgcgg ttaaacatct ggttaaacca ggcaaaaaag
    40501 cctttttgcc cgtgatgctc accttttttc agtggcttaa gtagtgtggc acacagagcc
    40561 ggagtgagga tcatcgctac cagtactgac agcaccatcg ccgcaacaat ggtaatagag
    40621 aactggcgat agatggcacc ggtggtgccg ccgaagaagg ccattggtac aaataccgcc
    40681 gaaagcacca tcgcaatccc gaccagtgcc ccctggatct gccccatcga tttacgcgtg
    40741 gcttcgcgag gagtgagtcc ttcctcactc ataatacgtt cgacgttttc caccaccacg
    40801 atggcgtcat ccaccagcag accgatcgcc agcaccatcg cgaacatggt taaggtgttg
    40861 acgctgtaac cgaaggcgta aagtacggag aaggttccca tcaacaccac cggcacggcg
    40921 atagtgggta tcagcgtggc gcggaagttt tgcaggaaca gatacataac gaggaaaacc
    40981 agagcgatag cttccagcag cgttttcacc acgtcttcaa tggaggcttt aacaaacgag
    41041 gtggtttcat acgccacctt gtattccagt ccgtgcggga aatactgcgc cagctcgtcg
    41101 agacgattga gcaccagctc cgctgtcgcc atttcgttcg cgccggaggc cagttttacc
    41161 cccaacccgg aggctggctt accattgaag cggctaagat aatcgtattt ctccgccccc
    41221 atttcggcgg tggcgacatc gccgagcctt acttctgagc cgtcctgatt cacccgcaag
    41281 gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat ggtggcgttg
    41341 agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat ctgcgcgttc
    41401 tgtgactcaa tggcatcagt gacatcttta gctgtcatct ggaaactgtt gagtttcgcc
    41461 gggtccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc cccgacgcca
    41521 tttacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcagcaat atcctgttta
    41581 tccatcgaac catcggtaga gacgaaggca atggtcagaa tgttggtatc gccggtttta
    41641 cgcacagtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc tgattgcagc
    41701 tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa acttaaagtg
    41761 acagatgcct gaccggtgcc actgctctga gatgacatat acatgagatt atcgaggccg
    41821 gtcatatttt gctcgataac ctgggtcacg gtgttttcca gcgtctgggc cgatgcgccg
    41881 ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg ttcaacgggc
    41941 aatgaaaaaa tcgccagggt acctgtcaga cacaacagga ttgccagcac ccaggcaaaa
    42001 atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc tgcgtatcgt
    42061 tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg agaaaaaaag
    42121 gagttaatgt aaattatcat gcccttttat actgaacctt aactgcaagt attctttggt
    42181 cagtaggagg cattacatca actgactttc ctcaccctcc gcagagcgaa agctaatact
    42241 cactaacgtg ccgccgccgg aaggttgtga aaaagtcagc gtcccaccta gccgttccgc
    42301 gcgttcgcgc atgatattca gaccataatg accttcgggt tctttcggtt caccgatacc
    42361 aatcccgtta tcacggatat aaaccgtgtg attgccgtcc ggcgcggtga cgcaactgac
    42421 ggcgatttcg ctggcgttgg cgtgcttcat cgcattcagc accgcttcgc gaataatttg
    42481 caacaaatgc acctgcattt gcgcatccag tgccagggtt ggcagacggc agtcgagggt
    42541 cagtttggcg ctggtttgat tttgtaacgt atccagcatt tccctcaacg cggaggggag
    42601 atccgcctgc tgcagcgtca ggcgaaaagt agtcaacagc tcgcgtaact gccgataagc
    42661 atcattcaac gcctgggaaa aatcggccat gatactttgt gcggtggcgt tatcttccgg
    42721 tatcgaacgc ttaagtaacg tcaactggat acgtaagtaa gaaagtacct gagccagcga
    42781 gtcgtgcaat tcgcgggcga tggttgcacg ttcttccatc aacaataact gctgaaaatg
    42841 cttctgcgcc tgattaaagt acaaaccgcg tcccagcatc gacgaaacgc tgttaagcag
    42901 cggttctgat gatgaaacgt gactattttg ccagtgcagt tcgccgtaaa ccgtctcttg
    42961 cattgtcacc ggtaaaatct gcatcggcaa ttccgggttt ggttgccctt cgctaatccg
    43021 ccagttttca ccgacattta actccagata ttcagccgct tcattgtcgc gaacaatctg
    43081 caaaatatgg cggaaacaat gcacatcaat ctggctggtg ttcagcgcct gcgaacattg
    43141 atacaacacc tccagacgac gcttggcctc gtggagatcg cgggtctttt cttctactga
    43201 cgcctccagc gaacggtaca atttatgcag ctcgctcgac atctggttaa aggtttttgc
    43261 aagtagacca agctcattcg gcaggctggt atccagcggt ggcgagtcga actgtccgtg
    43321 ttcaatacgc tgactggcgg taaccagctg attcagcggg gcaaccacct gatggcgtat
    43381 gcggcccaaa gtaaaaaaga ccagcgtgaa aataccgata ccgccagcca gggaaatcgc
    43441 caccaccagc agcattttgc gttcagcgta gtgctgtaaa gccagcacga acaggtctat
    43501 ctgattaaca taattattaa tattggcctg ataccacggc aaatcgccct tgctgagccg
    43561 attattcatc tccagccagt tggcattcag atgcgcatag cgagttttta ctgcttctgg
    43621 cacataccag acgttgaggt tggttaatac cggtgaatgc agtgcctgct gaaacagctg
    43681 gcgatgtgca ttgagttgtg gagtgccact ttgcaagtcg tagcccaggc ggtaactctg
    43741 catacgcagc gatccggcaa tattgatagc ctcagcgtcg cgcaaactgc tcgccagagt
    43801 tagcagagcg atacccgtgg aaagaatcga cagcagcaca atgtaaaaaa aggcccgggc
    43861 cagactggcc gagacgggtc gtttaacaat cacacgcgtc ttctccacaa aaaatcatta
    43921 atgttccagt tcgctgaaac gcaatttagt tagtgatggg attatttgat ctgccacagt
    43981 ttatagcgaa atagttactt attctgggca catgaacatt gccgattccc ctgtaagtcg
    44041 ggtaataaca acactcatat aaagaataag gtttttacaa ccaaaaaaga aggtcgttat
    44101 gaatcgtttt attatggcca acagtcagca atgtctgggt tgtcatgctt gtgaaatcgc
    44161 ttgtgtcatg gctcacaatg atgagcaaca tgtcctgagc caacaccatt ttcatccccg
    44221 aattacggtt atcaaacatc agcagcaacg tagtgcagtg acctgtcacc attgtgaaga
    44281 tgcgccctgc gcccgtagct gccctaatgg cgcaatcagc cacgttgatg acagcattca
    44341 ggtcaatcag caaaagtgta ttggctgtaa atcctgcgtg gtggcctgtc cgtttggtac
    44401 gatgcaaatc gtcctgacac ccgtcgcagc gggcaaagtt aaagccacgg cgcataaatg
    44461 cgacctttgt gcagggcgcg aaaacggtcc tgcctgtgtc gagaattgcc cggcggacgc
    44521 gctgcagctg gtcactgacg ccgcactctc aggcatggcg aaatcccgcc gcttgcgtac
    44581 cgcacgtcag gaacatcaac cgtggcatgc cagtaccgct gcacaagaaa tgccggtaat
    44641 gagtaaagtc gaacaaatgc aggcaacgcc cgcgcgtggc gagccggata aactggcgat
    44701 tgaagcgcgc aaaaccggtt ttgatgaaat ttatctgcca ttccgtgccg atcaggcaca
    44761 acgggaagcc tcgcgctgcc ttaaatgcgg cgagcacagc gtttgtgaat ggacctgccc
    44821 gctgcataac catataccgc agtggattga actggtgaaa gccggaaaca tcgacgccgc
    44881 cgtcgagctt tctcacctga ccaacaccct gccggaaatt accggacgcg tttgtccgca
    44941 agaccgtttg tgtgaaggtg cctgtactat tcgcgatgag cacggtgcgg taactatcgg
    45001 caacattgaa cgctacattt cagatcaggc gttggcgaaa ggttggcgtc ctgacttaag
    45061 ccatgtcacc aaagtggaca agcgggtggc gattatcggt gcaggtccgg cagggctggc
    45121 ctgtgcggat gttctgatcc gcaatggcgt ggcggtgacg gtgtacgatc gccatccaga
    45181 aatcggtggc ttgctcactt tcggcattcc ttctttcaaa ctggataaat ccctgctggc
    45241 acgtcgtcgg gaaatcttca gcgcgatggg gattcacttc gaactcaatt gtgaagtggg
    45301 taaagatgtc tctttggatt cgcttttgga acaatacgac gcggtcttcg ttggcgtagg
    45361 cacttaccgt tccatgaaag cgggtttacc caatgaagat gcgccgggcg tttatgacgc
    45421 gctgccgttc ctcatcgcca acactaaaca ggtgatgggg ctcgaagagc taccggaaga
    45481 gccgtttatc aataccgccg gacttaacgt cgtggtactg ggcggcggcg acaccgcgat
    45541 ggactgtgtg cgtactgcgc tgcgccacgg cgcgagtaac gtcacctgcg cttatcgtcg
    45601 tgatgaagct aacatgccag gctcgaagaa agaggtgaag aacgcccgcg aagagggggc
    45661 caacttcgaa tttaacgtcc agccggtggc gcttgagctg aatgaacaag gtcacgtctg
    45721 cgggattcgt ttcctgcgca cgcgtcttgg cgagccggat gcccaggggc gtcggcgtcc
    45781 agtgccggtg gaaggcagtg aatttgtcat gccagccgac gcggtgatta tggcgtttgg
    45841 cttcaatccg cacgggatgc cgtggctgga gtcgcacggt gtaacggtag acaaatgggg
    45901 ccgcatcatc gcggatgtgg aaagccagta ccgttaccag accaccaatc cgaaaatctt
    45961 cgccggtggc gacgccgtgc gtggtgcgga tctggtggtt accgcaatgg cagaaggacg
    46021 tcatgcggca caggggatta ttgactggct gggggtaaaa tcagtcaaat ctcactgata
    46081 gcctgcgcag acaaacccga cttcacagcg taatataatt gttcatttcg cgctgtggag
    46141 tcggtatgac gcaacaaatc accctcatta aagacaaaat tctctccgat aactatttca
    46201 ccctgcacaa cattacttac gatctcacgc gcaaagatgg cgaagttatc cgccataaac
    46261 gtgaagttta cgatcgcggt aatggtgcga cgatcctcct gtacaacgcg aagaaaaaga
    46321 ccgtggttct gattcgtcag ttccgtgtcg ctacctgggt taatggcaat gaaagcgggc
    46381 agctgattga aacctgcgcc gggctgctgg ataacgacga accggaagtg tgcattcgca
    46441 aagaagcgat tgaagagacg ggctatgaag tgggcgaagt gcgcaaatta tttgaactgt
    46501 atatgtcgcc aggcggtgtg actgagctaa tccacttttt tatcgccgaa tacagtgaca
    46561 atcagcgcgc taacgccggt ggcggtgtcg aagatgaaga tattgaagtg ctcgagctgc
    46621 cgttcagcca ggcgctggag atgatcaaaa ccggcgagat acgtgacggt aagacggtgt
    46681 tattgcttaa ctatttgcaa atgtcacatt taatagactg aaaaataaca ataatatttc
    46741 gttgtttatt cttggctcaa tccgacaaat ccgattgagc cgcgctacct gcgcaacgaa
    46801 gatacgactt gtgctgtttg tttgaacttc tggggtcgta ccgtccatgc gctatcgcat
    46861 tttccttctc ttttttttcg ctttgttgcc gacgtctttg gtgtgggcgg caccagcgca
    46921 acgggcgttt tccgactggc aggtcacctg taataaccaa aacttctgcg tggcgcgtaa
    46981 tacgggcgat cataatggac tggtgatgac cctgagccgc agcgccgggg cgcataccga
    47041 tgccgtttta cgtattgagc gcggcgggtt gaagtcgccg gatgcgtctg aaggggaaat
    47101 tgcgccacgg ctgctgttag atggcgagcc gttggcgtta agtggtgaca agtggcggat
    47161 ttcaccatgg ttattagtaa ccgatgatac ggcaaccatc accgcgtttt tgcagatgat
    47221 tcaggaaggg aaggcgatca ccttacgcga cggcaatcag accatttctc tgagtgactt
    47281 aaaagcggcg ttgttgttta ttgatgctca gcaaaagcgc gttggcagtg aaaccgcgtg
    47341 gatcaagaaa ggggacgaac cgccgctcag cgtaccgccc gcgcctgcgc tgaaagaggt
    47401 cgtggtggtt aacccaacgc cgacgccact ctcactcgaa gaacgcaacg atttgctgga
    47461 ttatggaaac tggcggatga atggtctgcg ctgctcgctt gatccattgc gtcgtgaggt
    47521 gaatgtcact gcgctgactg atgataaagc gctgatgatg attagctgtg aggcaggggc
    47581 ctataacacc attgatttgg catggattgt gtcgcgtaaa aagccgctgg cctcgcgccc
    47641 ggtccggctg cgtttgccgt tcaacaacgg tcaggagacg aatgaactgg aactgatgaa
    47701 cgcaacattt gatgagaaat cgcgtgaact ggtgacctta gcgaaagggc gcggattaag
    47761 cgattgtggc attcaggcgc gctggcgctt tgacggtcag cgttttcgcc tggtgcgtta
    47821 cgccgcagaa cccacctgcg ataactggca tgggccagac gcctggccca cgttgtggat
    47881 cacccgttaa acaaaatggc gggacatgta ggccggataa gacgctgtag gccggataag
    47941 acgcgtcagc gtcgcatccg gcaatcagca tccggcgatc accatcaggc accttttact
    48001 cccagcacct tatgcgcttt tgccacaata ttctcggcgg taaagccaaa gaacgggaac
    48061 agcttatccg ccggagcaga ttccccataa cccgtcatcc cgacaattgc ccctttcaga
    48121 ccaacatact tgtaccagta atcggcaata cctgcttcca ccgccacgcg agccgcaacg
    48181 ttagaaggca acaccgactc ccgatattcc tcatcctggg cgtcgaaaat atcggtcgag
    48241 ggcagggaaa ccacgcgaac attgcgacct tctcccgcta atttctccgc cgcttgcagg
    48301 gtgatttcca tctctgaacc ggtggcaatc aaaataatat ctggcttacc gccgctgtct
    48361 ttcagcacat aaccgccacg agcaatctct ttaacctgat ccggcgtacg ttccacctgg
    48421 gccagattct gcctggagag aatcagtgcc gtcggtccgt tgtggcgctc aaccgccagc
    48481 ttccagccca ccgccgcttc cacctgatcg cacggtcgcc aggtgctgaa atttggcgtt
    48541 aagcgcaggc tggccagttg ctcaacagcc tggtgcgtcg gaccatcttc gcccagacca
    48601 attgagtcgt gggtataaac cataatctgc cgcgctttca tcagtgccgc catccgcgcg
    48661 gcgttacggg cgtattcaac aaacatcagg aacgttgcgg tatacggcac aaagccgccg
    48721 tggtgcgcaa tgccgttggc gatagcggtc atgccaaatt cacgcacccc gtagtgaatg
    48781 tagttgcccg ccggatcttc cttcagcgaa acagaacctt tccagatggt caggttgctg
    48841 ggagccagat ccgccgaacc gccgagcagc tccggtagca tcggcccgta agcgttaagc
    48901 gtattttgcg aagccttacg ggtagcgatt ttcgccggat tcgcctgcaa ctcattgata
    48961 tatttctgag tcgttttctc ccagtccttc ggtaaaccac cgctcatccg acgggtaaac
    49021 tcttctgcca gttgcggatg agccttttta taggcggcaa acttctcatt ccagctctgc
    49081 tgcgcttttt cgcctttttc gcgggcatcc caagcgtgat aaatatcttt agggatctca
    49141 aatggcggat ggtgccagcc cagtttttgc cgtgccagcg ccacttcttc ttcccccagc
    49201 ggtgcgccgt gcgcctcttc cttacctgct ttattcggcg aaccaaagcc aatcaccgta
    49261 cggcagataa tcagcgacgg cttatctttc acgctttgcg cttcaaggat tgcttccttc
    49321 accgcctgcg gatcgtgacc gtcgatttca tggatcacat gccagtgata ggcttcaaaa
    49381 cgttttgccg tatcgtcggt aaaccagcct tcggtttctc catcaatcga aataccgttg
    49441 tgatcgtaaa aaccaatcag cttgcccagt cccagcgtgc ccgccagcga acagacttcg
    49501 tgggaaatac cttccatcag gcagccgtcg cccataaaca cataggtgaa gtgatcgaca
    49561 atttcatgat ccggctggtt aaactgcgcc gccagtgtac gttccgctat cgccagcccg
    49621 acggcgttcg ccaaaccttg tccaagagga ccggtggtgg tttcaactcc gggcgtatag
    49681 ccgatttccg ggtggccagg ggttttcgaa tgcagttgac gaaagttctt cagctcttcc
    49741 agcggcaggt cgtaaccggt cagatgcagc aaactgtaga gcagcatcga cgcgtgaccg
    49801 ttggaaagaa taaagcggtc gcgatcatac caggttgggt cggtagggtt atgtttaaga
    49861 aaatcgttcc acagcacttc ggcaatatca gccatgccca tcggcgcgcc gggatgacca
    49921 gaattggctt tttgtaccgc atccatactg agtgcgcgaa tcgcattggc aaggtctttt
    49981 cgggacatat aacactccgt ggctggttta tagtttggcg gcaagaagat cttccagttt
    50041 gcgttgatca acggcgaaca gacgaatgcc ttcagacagt ttttctaccg ccatcgcatc
    50101 ctgattgtgc tcccaacgga actccgcttc gctcatggga gctgggcgtg ggaacgtctg
    50161 agaaggtggg attaatttac gtaccactgg cgaaactttt tcctgcagct ccttcagcaa
    50221 attcggtgcg atagtcagtc gatcgcagcc ggttaaggcg aggatttgtt cggtgcgacg
    50281 gaagctcgcg cccatcacaa tggtttcata gtggtgttgc ttatagtagt cgtagatatt
    50341 gcgcaccgat ttaacgcccg gatcttcttc caccacatat gggtccatcg gcttgcgtgc
    50401 ctgataccag tcataaatac gcccgacaaa cggcgaaatc agaaaaacgc ccgcttctgc
    50461 acaggcccgc gcctgtgcaa aagaaaacag cagcgtcagg ttgcagttaa taccttcttt
    50521 ttccagctct tctgccgcgc gaattccttc ccaggtcgaa gccagtttga tcagaatgcg
    50581 tgatttctca acgccttgtt gctgatacaa gtccaccaga tggcgcgcct tctcaatact
    50641 cttttcttta tcaaaagaga ggcgtgcatc aacttctgtt gacacgcgac cgggtacgat
    50701 tttgaggatt tcagcaccga aattgaccgc cagtttgtca cacgctgcga ccacctgttg
    50761 ttcctgggtc ttgccatttt ttttacccca ggcgatagca tcgtctatta aatgctcata
    50821 ttgtgataat ccggcagcct tgagtaacag cgaaggattg gtggtggcat cctggggatg
    50881 ataatggcga atggactcaa tatcgccgct gtctgccacg acagtggtga actgtttgat
    50941 gccgtctaac tcgttcatag gaaatactcc ttgaaaagta aagtgttaga tgagtgcgtt
    51001 aattcacact tctgagaaat ttcgctaaac gcatcaaaaa agcatagcag acaggcatgg
    51061 tattgctgga ttaagcaggt aacatcagtg ttataggatt attaccaaaa cattatatta
    51121 atggtgagag tttggacttg ctcaaagtct gtagactccg gcaaggtaat aatgtgcgcc
    51181 acgttgtggg caggggctgt tgcccacaca ctttatttgt gaacgttacg tgaaaggaac
    51241 aaccaaatgg atgaccagtt aaaacaaagt gcacttgatt tccatgaatt tccagttcca
    51301 gggaaaatcc aggtttctcc aaccaagcct ctggcaacgc agcgcgatct ggcgctggcc
    51361 tactcaccag gcgttgccgc accttgtctt gaaatcgaaa aagacccgtt aaaagcctac
    51421 aaatataccg cgcgcggtaa cctggtggcg gtgatctcta acggtacggc ggtgctgggg
    51481 ttaggcaaca ttggcgcgct ggcaggcaaa ccggtgatgg aaggcaaggg cgttctgttt
    51541 aagaaattcg ctgggattga tgtatttgac attgaagttg atgaactcga cccggacaaa
    51601 tttattgaag tcgtcgctgc gctcgaaccg accttcggcg gcatcaacct cgaagacatt
    51661 aaagcgccag aatgtttcta tattgaacag aaactgcgcg agcggatgaa tattccggta
    51721 ttccacgacg atcagcacgg cacggcaatt atcagcactg ccgccatcct caacggcttg
    51781 cgcgtggtgg agaaaaacat ctctgacgtg cgtatggtgg tttccggcgc gggtgctgca
    51841 gcaatcgcct gtatgaacct gctggtagcg ttgggtctgc aaaaacataa catcgtggtt
    51901 tgcgattcaa aaggcgttat ctatcagggc cgtgagccaa acatggcgga aaccaaagcc
    51961 gcatatgcgg tagtggatga cggcaaacgt accctcgatg atgtgattga aggcgcagat
    52021 attttcctcg gctgttccgg cccgaaagtg ctgacccagg aaatggtgaa gaaaatggct
    52081 cgagcgccaa tgatcctggc gctggcgaac ccggaaccgg aaattctgcc gccgctggcg
    52141 aaagaagtgc gtccggatgc catcatctgt accggccgtt ccgactaccc gaaccaggtg
    52201 aacaacgtcc tttgcttccc gttcatcttc cgtggcgcgc tggacgttgg cgcaaccgcc
    52261 atcaacgaag agatgaaact ggcggcggta cgcgcgattg cagaactggc ccatgcggaa
    52321 cagagtgaag tggtggcttc agcgtatggc gatcaggatc tgagctttgg ccctgaatac
    52381 atcattccaa aaccgtttga tccgcgtctg atagtcaaga ttgctcctgc ggtcgcgaaa
    52441 gcggcgatgg agtcgggcgt ggcgacgcgt ccgattgctg atttcgacgt ctacatcgac
    52501 aagctgactg agttcgttta caaaaccaac ctgtttatga agccgatttt ctcccaggct
    52561 cgcaaagcgc cgaagcgcgt agttctgccg gaaggggaag aggcgcgcgt tctgcatgcc
    52621 actcaggaac tggtaacgct gggactggcg aaaccgatcc ttatcggtcg tccgaacgtg
    52681 atcgaaatgc gcattcagaa actgggcttg cagatcaaag cgggcgttga ttttgagatc
    52741 gtcaataacg aatccgatcc gcgctttaaa gagtactgga ccgaatactt ccagatcatg
    52801 aagcgtcgcg gcgtcactca ggaacaggcg cagcgggcgc tgatcagtaa cccgacagtg
    52861 atcggcgcga tcatggttca gcgtggcgaa gccgatgcaa tgatttgcgg tacggtgggt
    52921 gattatcatg aacattttag cgtggtgaaa aatgtctttg gttatcgcga tggcgttcac
    52981 accgcaggtg caatgaacgc gctgctgctg ccgagtggta acacctttat tgccgatacc
    53041 tacgtcaatg acgaaccaga cgcagaagag ctggcggaga tcaccttgat ggcggcagaa
    53101 actgtccgtc gtttcggtat tgaaccgcgc gttgctctgt tgtcgcactc caactttggt
    53161 tcttctgact gcccgtcgtc gagcaaaatg cgtcaggcgc tggaactggt cagggaacgt
    53221 gcgccagaac tgatgattga tggtgaaatg cacggcgatg cagcgctggt ggaagcgatt
    53281 cgcaacgacc gtatgccgga cagccctttg aaaggttctg ccaatattct ggtgatgccg
    53341 aacatggaag ctgcccgcat tagttacaac ttactgcgcg tttccagctc ggaaggtgtg
    53401 actgtcggtc cggtgctgat gggcgtggcg aaaccggttc acgtgttaac gccgatcgca
    53461 tcggtgcgtc gtatcgtcaa catggtggcg ctggccgtgg ttgaagcgca aacccaaccg
    53521 ctgtaattct tttttaactc tcacgcttac cctgaatatt cagggtaagc ggtttagctg
    53581 caatatatta gtaaagctta ttactgagtt
//
LOCUS       DAZKIF010000014        53063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZKIF010000014 DAZKIF010000000
VERSION     DAZKIF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.014"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            229..1323
                     /gene="gcvT"
                     /locus_tag="ACHOTW_000888"
     CDS             229..1323
                     /gene="gcvT"
                     /locus_tag="ACHOTW_000888"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP9843050.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1347..1736
                     /gene="gcvH"
                     /locus_tag="ACHOTW_000889"
     CDS             1347..1736
                     /gene="gcvH"
                     /locus_tag="ACHOTW_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP9843051.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1854..4727
                     /gene="gcvP"
                     /locus_tag="ACHOTW_000890"
     CDS             1854..4727
                     /gene="gcvP"
                     /locus_tag="ACHOTW_000890"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP9843052.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4893..6326)
                     /gene="bglA"
                     /locus_tag="ACHOTW_000891"
     CDS             complement(4893..6326)
                     /gene="bglA"
                     /locus_tag="ACHOTW_000891"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP9843053.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            6371..6682
                     /gene="yqfB"
                     /locus_tag="ACHOTW_000892"
     CDS             6371..6682
                     /gene="yqfB"
                     /locus_tag="ACHOTW_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP9843054.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            6846..7505
                     /gene="yqfA"
                     /locus_tag="ACHOTW_000893"
     CDS             6846..7505
                     /gene="yqfA"
                     /locus_tag="ACHOTW_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9843055.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(7582..8562)
                     /gene="ygfZ"
                     /locus_tag="ACHOTW_000894"
     CDS             complement(7582..8562)
                     /gene="ygfZ"
                     /locus_tag="ACHOTW_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP9843056.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            8805..9071
                     /gene="sdhE"
                     /locus_tag="ACHOTW_000895"
     CDS             8805..9071
                     /gene="sdhE"
                     /locus_tag="ACHOTW_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP9843057.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9052..9459
                     /gene="ygfX"
                     /locus_tag="ACHOTW_000896"
     CDS             9052..9459
                     /gene="ygfX"
                     /locus_tag="ACHOTW_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP9843058.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9499..10020)
                     /gene="fldB"
                     /locus_tag="ACHOTW_000897"
     CDS             complement(9499..10020)
                     /gene="fldB"
                     /locus_tag="ACHOTW_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP9843059.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10132..11028
                     /gene="xerD"
                     /locus_tag="ACHOTW_000898"
     CDS             10132..11028
                     /gene="xerD"
                     /locus_tag="ACHOTW_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9843060.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTLWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11053..11763
                     /gene="dsbC"
                     /locus_tag="ACHOTW_000899"
     CDS             11053..11763
                     /gene="dsbC"
                     /locus_tag="ACHOTW_000899"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP9843061.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            11769..13502
                     /gene="recJ"
                     /locus_tag="ACHOTW_000900"
     CDS             11769..13502
                     /gene="recJ"
                     /locus_tag="ACHOTW_000900"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9843062.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13593..14691
                     /gene="prfB"
                     /locus_tag="ACHOTW_000901"
     CDS             join(13593..13667,13669..14691)
                     /gene="prfB"
                     /locus_tag="ACHOTW_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9843063.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14701..16218
                     /gene="lysS"
                     /locus_tag="ACHOTW_000902"
     CDS             14701..16218
                     /gene="lysS"
                     /locus_tag="ACHOTW_000902"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9843064.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16261..16809)
                     /gene="idi"
                     /locus_tag="ACHOTW_000903"
     CDS             complement(16261..16809)
                     /gene="idi"
                     /locus_tag="ACHOTW_000903"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9843065.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            16864..16935
                     /locus_tag="ACHOTW_000904"
     CDS             16864..16935
                     /locus_tag="ACHOTW_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843066.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(16932..17057)
                     /gene="yqfG"
                     /locus_tag="ACHOTW_000905"
     CDS             complement(16932..17057)
                     /gene="yqfG"
                     /locus_tag="ACHOTW_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP9843067.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17059..18507)
                     /gene="uacT"
                     /locus_tag="ACHOTW_000906"
     CDS             complement(17059..18507)
                     /gene="uacT"
                     /locus_tag="ACHOTW_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP9843068.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18943..20862
                     /gene="ygfT"
                     /locus_tag="ACHOTW_000907"
     CDS             18943..20862
                     /gene="ygfT"
                     /locus_tag="ACHOTW_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP9843069.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20862..21350
                     /gene="ygfS"
                     /locus_tag="ACHOTW_000908"
                     /pseudo
     CDS             20862..21350
                     /gene="ygfS"
                     /locus_tag="ACHOTW_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(21386..22753)
                     /gene="ghxQ"
                     /locus_tag="ACHOTW_000909"
                     /gene_synonym="ygfQ"
     CDS             complement(21386..22753)
                     /gene="ghxQ"
                     /locus_tag="ACHOTW_000909"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP9843070.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22789..24105)
                     /gene="guaD"
                     /locus_tag="ACHOTW_000910"
     CDS             complement(22789..24105)
                     /gene="guaD"
                     /locus_tag="ACHOTW_000910"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP9843071.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24123..25523)
                     /gene="xanQ"
                     /locus_tag="ACHOTW_000911"
     CDS             complement(24123..25523)
                     /gene="xanQ"
                     /locus_tag="ACHOTW_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP9843072.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(25688..28558)
                     /gene="xdhD"
                     /locus_tag="ACHOTW_000912"
     CDS             complement(25688..28558)
                     /gene="xdhD"
                     /locus_tag="ACHOTW_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP9843073.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28555..29334)
                     /gene="ygfM"
                     /locus_tag="ACHOTW_000913"
     CDS             complement(28555..29334)
                     /gene="ygfM"
                     /locus_tag="ACHOTW_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP9843074.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29384..30712)
                     /gene="ssnA"
                     /locus_tag="ACHOTW_000914"
     CDS             complement(29384..30712)
                     /gene="ssnA"
                     /locus_tag="ACHOTW_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP9843075.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30715..33813)
                     /gene="ygfK"
                     /locus_tag="ACHOTW_000915"
     CDS             complement(30715..33813)
                     /gene="ygfK"
                     /locus_tag="ACHOTW_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP9843076.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34135..34713)
                     /gene="mocA"
                     /locus_tag="ACHOTW_000916"
     CDS             complement(34135..34713)
                     /gene="mocA"
                     /locus_tag="ACHOTW_000916"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP9843077.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            34816..35586
                     /gene="yqeC"
                     /locus_tag="ACHOTW_000917"
     CDS             34816..35586
                     /gene="yqeC"
                     /locus_tag="ACHOTW_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP9843078.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            35634..37259
                     /gene="yqeB"
                     /locus_tag="ACHOTW_000918"
     CDS             35634..37259
                     /gene="yqeB"
                     /locus_tag="ACHOTW_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP9843079.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(37300..38232)
                     /gene="arcC"
                     /locus_tag="ACHOTW_000919"
     CDS             complement(37300..38232)
                     /gene="arcC"
                     /locus_tag="ACHOTW_000919"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9843080.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(38280..39665)
                     /gene="hyuA"
                     /locus_tag="ACHOTW_000920"
     CDS             complement(38280..39665)
                     /gene="hyuA"
                     /locus_tag="ACHOTW_000920"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP9843081.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(39718..40929)
                     /gene="ygeY"
                     /locus_tag="ACHOTW_000921"
     CDS             complement(39718..40929)
                     /gene="ygeY"
                     /locus_tag="ACHOTW_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP9843082.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(40987..42183)
                     /gene="dpaL"
                     /locus_tag="ACHOTW_000922"
     CDS             complement(40987..42183)
                     /gene="dpaL"
                     /locus_tag="ACHOTW_000922"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP9843083.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(42241..43428)
                     /gene="ygeW"
                     /locus_tag="ACHOTW_000923"
     CDS             complement(42241..43428)
                     /gene="ygeW"
                     /locus_tag="ACHOTW_000923"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP9843084.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            43907..45685
                     /gene="ygeV"
                     /locus_tag="ACHOTW_000924"
     CDS             43907..45685
                     /gene="ygeV"
                     /locus_tag="ACHOTW_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9843085.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(45725..46204)
                     /gene="xdhC"
                     /locus_tag="ACHOTW_000925"
     CDS             complement(45725..46204)
                     /gene="xdhC"
                     /locus_tag="ACHOTW_000925"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP9843086.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(46201..47079)
                     /gene="xdhB"
                     /locus_tag="ACHOTW_000926"
     CDS             complement(46201..47079)
                     /gene="xdhB"
                     /locus_tag="ACHOTW_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP9843087.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(47090..49387)
                     /gene="xdhA"
                     /locus_tag="ACHOTW_000927"
     CDS             complement(47090..49387)
                     /gene="xdhA"
                     /locus_tag="ACHOTW_000927"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP9843088.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKTFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            49803..50558
                     /gene="actS"
                     /locus_tag="ACHOTW_000928"
     CDS             49803..50558
                     /gene="actS"
                     /locus_tag="ACHOTW_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP9843089.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            50637..50710
                     /locus_tag="ACHOTW_000929"
     tRNA            50637..50710
                     /locus_tag="ACHOTW_000929"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50669..50671,aa:Gly,seq:ccc)
     gene            50944..52549
                     /locus_tag="ACHOTW_000930"
                     /pseudo
     CDS             50944..52549
                     /locus_tag="ACHOTW_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
     gene            52616..52795
                     /locus_tag="ACHOTW_000931"
     CDS             52616..52795
                     /locus_tag="ACHOTW_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFP9843090.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT        13152 a        13088 c        13763 g        13060 t
ORIGIN      
        1 aaacttgtgt attttttggt tttttattct gttgcgattt tggcgttttt taaccataag
       61 ctaatgtgat gatcaatttt accttatggt taacagtctg tttcggtggt aagttcaggc
      121 aaaagagaac gattgcgttg gggaccggga gtggctccga tgctgggttt cgtggtgata
      181 atttcaccat gaaaaagttg tcagccccgc ttattcaatg aggacaagat ggcacaacag
      241 actcctttgt acgaacaaca cacgctttgc ggcgctcgca tggtggattt ccacggctgg
      301 atgatgccgc tgcattacgg ttcgcaaatc gacgaacatc atgaggtacg taccgatgcc
      361 ggaatgtttg atgtgtcaca tatgaccatc gtcgatcttc gcggcagccg cacccgggag
      421 tttctgcgtt atctgctggc gaacgatgtg gcgaagctca ccaaaagcgg caaagccctt
      481 tactcgggga tgttgaatgc ctctggcggt gtgatagatg acctcatcgt ctactacttt
      541 actgaagatt tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga cctctcctgg
      601 attacccaac acgctgaacc tttcggcatc gaaattaccg ttcgtgatga cctttccatg
      661 atcgccgtac aagggccgaa tgcgcaggca aaagctgcca cactgtttaa tgatgctcag
      721 cgtcaggcgg tggaagggat gaaaccgttc tttggcgtgc aggcgggcga tctgtttatt
      781 gccaccactg gttataccgg tgaagcgggc tatgaaattg cgctgcccaa tgaaaaagcg
      841 gccgatttct ggcgtgcgct ggtggaaggg ggcgttaagc catgcggtct gggcgcgcgt
      901 gacacgctgc gtctggaagc gggcatgaat ctttatggtc aggagatgga cgaaaccatc
      961 tctccgttag ccgccaacat gggctggact atcgcctggg aaccggcaga tcgtgacttc
     1021 atcggtcgtg aagccctgga agtgcagcgt gagcatggca cagaaaaact ggttggtctg
     1081 gtgatgaccg aaaaaggcgt gctgcgtaat gaactgccgg tacgttttac cgatgcgcag
     1141 ggcaaccagc atgaaggcat tatcaccagc gggactttct ccccgacgct gggttacagc
     1201 attgcgctgg cacgcgtgcc gaaaggtatt ggcgaaacgg cgattgtgca aattcgcaac
     1261 cgtgaaatgc cggttaaagt gacaaaacct gtttttgtgc gtaacggcaa agccgtcgcg
     1321 tgatttactt ttttggagat tgattgatga gcaacgtacc agcagaactg aaatacagca
     1381 aagaacacga atggctgcgt aaagaagccg acggcactta caccgttggt attaccgaac
     1441 atgctcagga gctgttaggc gatatggtgt ttgttgacct gccggaagtg ggcgcaacgg
     1501 ttagcgcggg cgatgactgc gcggttgccg aatcagtaaa agcggcgtca gacatttatg
     1561 cgccagtaag cggtgaaatc gtggcggtaa acgacgcact gagcgactcc ccggaactgg
     1621 tgaacagcga accgtatgca ggtggctgga tctttaaaat caaagccagc gatgaaagcg
     1681 aactggaatc actgctggat gcgaccgcat acgaagcatt gttagaagac gagtaacggc
     1741 tttattcctc ttctgcggga gaggatcagg gtgaggaaat ttacacctca ccctcactct
     1801 cttcgcaagg agagaggttc acaattcact gcacgtttca ggaaccatcg ctcatgacac
     1861 agacgttaag ccagcttgaa aatagcggcg cttttattga acgccatatc ggaccggacg
     1921 ccgcgcaaca gcaagaaatg ctgaatgccg ttggcgcaca atcgttaaac gcgctgaccg
     1981 gccagattgt accgaaagat attcagcttg cgaccccgcc gcaggttggc gcaccggcga
     2041 ccgaatacgc cgcgctggcg gaactcaagg ctattgccag tcgcaataaa cgcttcacgt
     2101 cttacatcgg catgggttac accgccgtgc agttaccgcc ggttatcctg cgtaacatgc
     2161 tggaaaatcc gggctggtat accgcgtata ctccgtatca acctgaagtc tcgcagggcc
     2221 gccttgaggc actgctcaat ttccagcagg taacgctgga tttaaccggg ctggatatgg
     2281 cctctgcttc tcttcttgac gaagccaccg ctgccgccga agcaatggcg atggcgaaac
     2341 gcgtcagcaa actgaaaaat gccaaccgct tcttcgtggc ttccgatgtg catccgcaaa
     2401 cgctggatgt ggtccgtact cgtgccgaaa cctttggttt tgaagtgatt gtcgatgatg
     2461 cgcaaaaagt gctcgaccat caggacatct tcggcgtgct gttacagcag gttggcacta
     2521 ccggtgaaat tcacgactac actgcgctta tcagcgaact gaaatcacgc aaaattgtgg
     2581 tcagcgttgc cgctgatatt atggcgctgg tgctgttaac tgcgccgggt aaacagggcg
     2641 cggatattgt ttttggttcg gcgcaacgct ttggcgtacc gatgggttac ggtggcccac
     2701 acgcggcatt ctttgcggcg aaagacgaat acaaacgctc aatgccaggc cgtattatcg
     2761 gtgtatcgaa agatgcagct ggcaataccg cgctgcgcat ggcgatgcag actcgcgagc
     2821 aacatattcg tcgtgagaaa gcgaactcca acatttgtac ttctcaggtg ctgctggcaa
     2881 acatcgccag cctgtatgcc gtttatcacg gcccgattgg cctgaaacgt atcgctaacc
     2941 gcattcaccg tctgaccgat atcctggcgg caggcctgca acaaaaaggt ctgaagctgc
     3001 gccatgcgca ctatttcgac actctgtgtg tggaagtggc cgacaaggcg ggcgtactgg
     3061 cgcgtgccga agcggctgaa atcaacctgc gtagcgatat cctgaacgcg gttgggatca
     3121 cccttgatga aaccaccacg cgcgaaaacg tgatgcagct tttcagcgta ctactggggg
     3181 ataaccacgg gctggacatc gacacgctgg acaaagacgt ggctcacgac agccgctcta
     3241 tccagcctgc gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt aatcgctacc
     3301 acagcgaaac cgaaatgatg cgctatatgc actcgctgga gcgtaaagat ctggcgctga
     3361 atcaggcgat gatcccgctg ggttcctgca ccatgaaact gaacgccgcc gccgagatga
     3421 tcccaatcac ctggccggaa tttgccgaac tgcacccgtt ctgcccgccg gagcaggccg
     3481 aaggttatca gcagatgatc gcgcagctgg ctgactggct ggtgaaactg accggttacg
     3541 acgccgtttg tatgcagccg aactctggcg cacagggcga atacgcgggc ctgctggcga
     3601 ttcgtcatta tcatgaaagc cgcaacgaag ggcatcgcga tatctgcctg atcccggctt
     3661 ctgcgcacgg cactaacccc gcttctgcac atatggcagg aatgcaggtg gtggttgtgg
     3721 cgtgtgataa aaacggaaat atcgatctga ctgatctgcg cgcgaaagcc gaacaggcag
     3781 gtgataatct ctcctgcatc atggtgacct atccttccac ccacggtgtg tatgaagaaa
     3841 cgatccgtga agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac cttgatggcg
     3901 cgaacatgaa cgcccaggtt ggcatcacct cgccgggctt tattggcgca gacgtttcac
     3961 accttaacct gcataaaact ttctgcattc cgcacggcgg tggtggtccg ggtatgggac
     4021 cgatcggcgt gaaagcgcat ctggctccgt ttgtaccggg tcatagcgtg gtgcaaatcg
     4081 aaggcatgtt aacccgtcag ggcgcggttt ctgcggcacc gttcggtagc gcctctatcc
     4141 tgccaatcag ctggatgtac atccgcatga tgggcgcaga agggctgaaa aaagcaagcc
     4201 aggtggcaat cctcaacgcc aactatattg ccagccgcct gcaggatgcc ttcccggtgc
     4261 tgtataccgg tcgtgacggt cgcgtggcgc acgaatgtat tctcgatatt cgtccgttga
     4321 aagaagaaac cggcatcagc gagctggata ttgccaagcg cctgatcgac tacggtttcc
     4381 acgcgccgac gatgtcgttc ccggtggcag gtacgctgat ggttgaaccg actgaatctg
     4441 aaagcaaagt ggaactggat cgctttatcg acgcgatgct ggctatccgc gcagaaattg
     4501 accaggtgaa agccggtgtc tggccgctgg aagataaccc gctggtgaac gcgccgcaca
     4561 ttcagagtga actggtcgcc gagtgggcgc atccgtacag ccgtgaagtt gcggtattcc
     4621 cggcaggcgt ggcagacaaa tactggccga cagtgaaacg tttggatgat gtttacggcg
     4681 accgtaacct gttctgctcc tgcgtaccga ttagcgaata ccagtaattc actgattcga
     4741 ctatcttcta aaggcgcttc ggcgcctttt tagattgtta ataaagtgcg ctttgtgtgt
     4801 ttcggctgag gcgtaatata aaatattgcg agctcaatat attaaaagag tgccaggcct
     4861 gatgagcgta gcgcatcagg cacatcggct aattacagat tctcgccgtt gctggcaatc
     4921 acttctttgt accagttaaa gctcttctta cgtgaacgcg acatatcgcc agtaccgtcg
     4981 tcatgtttat tcacatagat aaagccgtag cgtttgctgt actgcccggt agtgaacgac
     5041 acgcagtcga tgcagcccca cggtgtgtag cccatcagat ccacgccatc gtaagttact
     5101 gcttttttca tctcttcaat atgggcgcgc aggtagtcaa tgcggtagtc gtcgttgatg
     5161 ctgccatctt cttccacttt gtcgtaagcg ccaaaaccgt tttcgacaat aaacagcggc
     5221 ttctggtaac gctcatacag ttcgcaaagt gcgtaacgca gacccaccgg atcaatctgc
     5281 cagccccagt cagatgcttt aacatacggg tttggtacgc tgccttcaaa accagagatc
     5341 gcatcgccgg tgccgccttc ggccttcact gcgttggtca tgtaatagct gaaaccaaga
     5401 taatcgcagg tgccttcgcg cagtacatcg agatcgctat cttccatttt gatgttaaat
     5461 ccgcgacgct cccactcgtt caacacatac gacgggtaat agccgcgtag ctgcacatcg
     5521 gtaaagacgt agcgttcgcg catcgactcc tgagcgaaca tcacatcgtc cgggttacat
     5581 gagtagggat agagcggcac catcgccagc atacagccga ctttcatctc caggttaatg
     5641 cgacgcgcgg ctttcactgc cagggcgctg gcgacaaact ggtgatgcag cacctgatac
     5701 atcgtctctt ccgggttttc atgctcggta tacaccacgc cggagcagca gtaaccgaac
     5761 agcggtgcac gccagttacg ctggttgtta atttcgttga aggtcatcca gtatttgact
     5821 ttgtgcttat agcgttcaaa taccacttcc gcgaaacgta caaagaaatc aaccacttta
     5881 cggttggtcc agctaccgta ttgctgcacc agatgcagcg gcatttcaaa gtgggagagg
     5941 gtgatcaccg gttcgatgtt gtatttcagc agttcatcga acagagagtc gtagaacttc
     6001 agcccttctt cgtttggctg agcttcatcg ccttttggaa aaatgcgcgt ccaggcaatg
     6061 gatgtacgaa aacatttgaa gcccatttcg gcaaatagct tgatgtcttc cttatagtga
     6121 ccataaaaat caacggcttc atggtttgga tagtatttgc ctggcaagac ttctttggtt
     6181 atttcgcgcg gcacgccgtg tgcgccaccg gtcagaacgt cacaaatgct cggccctttt
     6241 ccgcctttgt tccagccgcc ttcgacctga tgagcggcaa ctgcgccgcc ccataagaaa
     6301 tctttcggta aggtgagttt tttcactatc ataggctcca gatgagtcaa ttcttacagg
     6361 aaatagtacc atgcagccaa acgacatcac tttttttcaa cgtttccagg atgacattct
     6421 ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga
     6481 tgtgcttcgt gtcggacgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc
     6541 aacctcaacc gtaacgctgg atacgctgac ggaaaaacat gcagagcagg aaaatatgac
     6601 cctgactgaa ctgaaaaagg tcattgccga catctatccc ggccagacac agttttatgt
     6661 gattgaattt aaatgccttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa
     6721 tttcatgatt tttaggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca
     6781 ctggaactat tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctccggagt
     6841 aagttatggt tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca
     6901 acagcgtcag tcacggcatt gggttggtgt ttggtatcgt tgggctggtg ttgctactgg
     6961 ttcaggcggt ggatctcaat gccagcgcca cagcgataac cagctacagc ctctatggcg
     7021 gcagtatgat cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg
     7081 caaaaatgtg gctgaagaaa tttgaccatt gcgctattta cctgttgatt gccggaacct
     7141 acacgccgtt tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta
     7201 tctggagcct ggcattgctg gggattctgt tcaaactgac catcgcgcac cgattcaaaa
     7261 ttttatctct ggtgacttat ctggcgatgg gctggctgtc gctggtggta atttatgaaa
     7321 tggcagttaa gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc gtggtttatt
     7381 cgctcggggt gattttctac gtctgcaaac gcattccata caaccatgcc atctggcacg
     7441 gcttcgtgct cggcggtagt gtgtgccact ttctggcgat ctatttgtat attgggcagg
     7501 cgtaatgggt atgcctttgt aggcctgata agacgcgcca gcgtcgcatc aggcgataaa
     7561 tgcctgatgc gacacagaga tttactcttc gagcgaatac ggcagtggct cgatacacaa
     7621 tgtattcgca tcgtcgcgca cgcggaagat gctgtccggt tccatatcgt tattcatcac
     7681 tacctgcacc acgacctgac catcttccag ttttaccgca gccagcaccg taccagtacg
     7741 acgccagttc tcgcccattt tcagctctaa gtcttcacca gcttccggca gtcggctggc
     7801 actacctgtc agcaaccaga gcgcgcgttt gttggcacca cggaattttg ctcgcgccac
     7861 catctcttgt ccggtatagc agcctttctt aaagctgata ccgcccagcg cctggaggtt
     7921 ggtcgcctgt gggataaact gcccgctgtt cgcggcatca atcaccggga aacccgcttc
     7981 aatgtttaat gccagccact gttggctatt gttcagttcc gcttcaccgc gcagtttatc
     8041 ggtcagcata ttggcagtag cttcatcggt tacgatcagg aaacgttctg ccgggtgttc
     8101 aaaccatagc aaagtggtcg cgccttcttt aactacctgt ttttctttcg aaggcagttc
     8161 actaaagata tttgccagcg cggcgcgcgc ctgaaaaccg gcaacaccaa gcagcacacg
     8221 ctcgtcgtct ggcgcgatgg tcactttaga gaacaccgca tattttttca gttcagccag
     8281 ctgcggttca cgcacgctgc gccgttcaat ccatgcaaag ccatcgccgt cgcggaacag
     8341 acgtaaattg ctccacattt tacctttggc gtcgcaatgg gcggcgagca ggtgctgatc
     8401 atctgtcatc tggctgacat ctgctgtcac ctggccctgc aaatattttt cgctgtccgc
     8461 gccagtaatg gtggcaagcg cccagtcatc aagcgtcatc agcgtcagtg gcaaacgggc
     8521 agaagccgta ggctgacggg gaggaaaagg tgtaaaagcc ataatagagt cctgagttgc
     8581 ttaacggcgt attaataact aatggtaaaa gagctatgag gcaatgcaag tgctttcact
     8641 acgcgatctg tgccttctgc gaggcgactt cccaaaaaat gaagccctta aattgttgtg
     8701 aattaatttc gcgatcgcga ttgtgtttac tgatattccc gtaaaagaag tcgaaaaaca
     8761 cgttacaata gccgggtact ggattttcgt gagacaggaa gaagatggac attaacaaca
     8821 aagcccgcat tcattgggca tgccgccgtg gtatgcgcga actcgatatt tcaatcatgc
     8881 cgtttttcga acatgagtat gacagcttaa gcgatgacga aaaacgcatc tttattcgtc
     8941 tgctggaatg tgacgatccc gatctgttta actggctgat gaatcacggt aaaccggccg
     9001 atgcagaact ggaaatgatg gtccgactca tccagacacg gaaccgggaa cgtggtcctg
     9061 tggcaatctg atttgcgcgt ctcctggcgc gcacagtggc tttccttgct gattcatggg
     9121 ctggttgccg ctgttatttt actcatgccc tggccgctca gttacacccc gttatggatg
     9181 gtgttacttt cgctggtggt gtttgattgc gttcgcagcc agcggcgtat taatgctcgc
     9241 cagggggaaa ttcgcttgtt gatggacggg cgtttgcgtt ggcaagggca ggagtggagc
     9301 atcgtcaaag cgccgtggat gattaagagc ggcatgatgc tgcgtttacg ttctgatagc
     9361 ggtaaacggc aacatttatg gctggcagcc gacagcatgg acgaagccga atggcgggat
     9421 ttacggcgga ttttgttgca acaagagacg caaagataaa gatgacggca aagcatatgc
     9481 gctaagccgt cagtgatatc aggcgtaatg ctctgccatt tcgttgagga tttgctcgca
     9541 ccagctctga atacgctcgt cgctaaggtc atactggtta gtttcgtcca gcgccagccc
     9601 cacgaacagt tgcccgtcag caatcaccgg tttcgggctg gtaaattcat acccttccgt
     9661 tggccagtag ccgacgaact tcacgccttt ggtcgagagt ttgtcatgca gcataccgag
     9721 cgcatcgagg aaccactcgc cgtatcccag ttgatcgcca agcccataca gcgcaacaat
     9781 tttaccttcg aggttcaggt cgtcgagctg atcccagacg gcttcccagt cttcctggat
     9841 ttcgccaaaa tcccaggtcg ggatacccag aatgagcaca tcgtactgct ccattaattt
     9901 cggggagtcg tccttgaggt tatgtaaggt caccagttct gggccgataa tatcgcggat
     9961 tttttctgcc gccatttcgg tgtaacaggt gctggaaccg taaaaaagac ccatattcat
    10021 aacgtaagct tctcgattct gctgtggctt ttgcggctag tgtatcagaa tcgctttagt
    10081 cacggcacaa taaggcataa tggatcaaat gagtgaccat aaggggcgca agtgaaacag
    10141 gatctggcac gcatcgagca gtttcttgat gctctgtggc tggaaaaaaa tctggctgaa
    10201 aatacgttga acgcttaccg tcgcgatctg tcaatgatgg tggagtggtt gcatcaccgc
    10261 gggttgacgc tggcgacggc gcaaagtgac gatttacagg cattactggc agaacggctg
    10321 gagggcgggt ataaagccac cagctcagcg cgtttgctga gtgcagtgcg ccgattgttc
    10381 cagtatcttt atcgcgaaaa gtttcgtgaa gatgatccca gtgcgcatct cgcttcaccg
    10441 aaattgcccc agcgtttgcc aaaagattta agcgaagcgc aggtcgaacg tttattacag
    10501 gcaccattaa ttgatcagcc actggagcta cgcgataaag ctatgcttga agtgttgtat
    10561 gctaccgggc tgcgtgtctc tgaactggtc gggctgacaa tgagtgatat cagcctgcgt
    10621 cagggcgtag tgcgggtcat tggtaaaggc aacaaagagc gcctggtgcc gttaggtgaa
    10681 gaggcggttt actggctgga aacctatctg gaacatgggc gtccgtggct actgaatggt
    10741 gtgtcaattg acgtgttgtt tcccagccag cgtgcgcagc agatgacgcg acagacctta
    10801 tggcaccgca ttaaacatta tgccgtgctg gcgggtatcg acagcgaaaa gctgtcaccg
    10861 catgtgttgc gtcacgcttt tgccactcac ttattaaatc atggtgcgga tttacgcgtg
    10921 gtgcagatgc tactgggcca cagcgatctc tccaccacgc aaatttatac gcatgtcgct
    10981 accgagcgtc tgcggcaact tcatcaacag catcacccgc gggcgtgatg tctgaaaaga
    11041 acgggaagat ttatgaagaa aggttttatg ttgtttactt tgttagcggc gttttcaggc
    11101 ttcgctcagg ctgatgacgc ggcaattcaa caaacattag ccaaaatggg catcaaaagc
    11161 agcgatattc agcccgcgcc cgtagctggc atgaagactg tgctgactaa cagcggtgtg
    11221 ttgtacatca ccgatgatgg taaacatatc attcaggggc caatgtatga cgttagcggc
    11281 acggctccgg tcaatgtcac caataagatg ctgttaaagc agttgaatgc gcttgaaaaa
    11341 gagatgatcg tttataaagc accgcaggaa aaacacgtca tcaccgtgtt tactgatatt
    11401 acctgtggtt actgccacaa actgcatgaa caaatggcag actataacgc gctggggatc
    11461 accgtgcgtt atcttgcttt cccacgccag gggctggaca gcgatgctga gaaagaaatg
    11521 aaagctatct ggtgtgcgaa agataaaaac aaagcgtttg atgatgtgat ggcaggtaaa
    11581 agcgtcgcac cagccagctg cgacgtggat attgccgacc attacgcact tggcgtccag
    11641 cttggcgtta gcggtactcc ggcagttgtg ctgagcaatg gcacacttgt tccgggttac
    11701 cagccgccga aagagatgaa agaattcctc gacgaacacc aaaaaatgac cagcggtaaa
    11761 taattcgcgt gaaacaacag atacaacttc gtcgccgtga agtcgatgaa acggcagact
    11821 tgcccgcgga attgcctccc ttgctgcgcc gtttatacgc cagccgggga gtacgcagtg
    11881 cgcaagaact ggaacgcagt gttaaaggta tgctgccctg gcagcaactg agcggcgtcg
    11941 aaaaggccgt tgagatcctt tacaacgctt ttcgcgaagg aacgcggatt attgtggtcg
    12001 gtgatttcga cgccgacggt gccaccagca cggctctaag cgtgctggcg atgcgctcgc
    12061 ttggttgcag caatatcgac tacctggtac caaaccgttt cgaagacggt tacggcttaa
    12121 gcccggaagt ggtcgatcag gcccatgccc gtggcgcgca gttaattgtc acggtggata
    12181 acggtatttc ctcccatgcg ggagttgagc acgctcgctc gttgggcatc ccggttattg
    12241 ttaccgatca ccatttgccg ggcgacacat tacccgcagc ggaagcgatc attaacccta
    12301 acttgcgcga ctgtaatttc ccgtcgaaat cactggcagg cgtgggtgtg gcgttttatc
    12361 tgatgctggc gctgcgcacc tttttgcgcg atcagggctg gtttgatgag cgcggcatcg
    12421 caattcctaa cctggcagaa ctgctggatc tggtcgcgct ggggacagtg gcggacgtcg
    12481 tgccgctgga cgctaataat cgcattctga cctggcaggg gatgagtcgc atccgtgccg
    12541 gaaagtgccg tccagggatt aaagcgctgc tggaagtggc aaaccgtgat gcacaaaaac
    12601 tcgccgccag cgatttaggt tttgcgctgg ggccacgtct caatgctgcc gggcgactgg
    12661 acgatatgtc cgtcggtgtg gcgctgttgc tgtgcgacaa catcggcgaa gcgcgcgtgc
    12721 tggcaaatga actcgatgcg ctaaatcaga cgcgaaaaga gatcgaacaa ggaatgcaga
    12781 ttgaagcctt gaccctgtgc gagaaactgg agcgaagtcg cgacacgcta cccggcgggc
    12841 tggcaatgta tcaccccgaa tggcatcagg gcgttgtcgg tattctggct tcgcgcatca
    12901 aagagcgttt tcaccgtccg gttatcgcct ttgcgccagc aggtgatggt acgctgaaag
    12961 gttccggtcg ctccattcag gggctgcata tgcgtgatgc gctggagcga ttagacacac
    13021 tctaccccgg tatgatgctg aagtttggcg gtcatgcgat ggcggcgggt ttgtcgctgg
    13081 aagaggataa attcgaactc tttcaacaac ggtttggcga gttggttacc gagtggctgg
    13141 acccttcgct attgcaaggc gaagtggtgt cagacggccc gttaagcccg gccgaaatga
    13201 ccatggaagt ggcgcagctg ctgcgcgatg ctggcccgtg ggggcagatg ttcccggagc
    13261 cgctgtttga tggtcatttc cgtctgctgc aacagcggct ggtgggcgaa cgtcatttga
    13321 aagtcatggt cgaaccggtc ggcggcggtc cgctgctgga tggtattgct tttaatgtcg
    13381 ataccgccct ctggccggat aacggcgtgc gcgaagtgca actggcttac aagctcgata
    13441 tcaacgagtt tcgcggcaac cgcagcctgc aaattatcat cgacaatatc tggccaattt
    13501 agcgtcatct tctctataaa aaagagcgtg gattgggtac aatcccgctc ttatcaccgc
    13561 attttgacta gctcaataaa agaaatcaga ccatgtttga aattaatccg gtaaataatc
    13621 gcattcagga cctcacggaa cgctccgacg ttcttagggg gtatctttga ctacgacgcc
    13681 aagaaagagc gtctggaaga agtaaacgcc gagctggaac agccggatgt ctggaacgaa
    13741 cccgaacgcg cacaggcgct gggtaaagag cgttcctccc tcgaagccgt tgtcgacacc
    13801 ctcgaccaaa tgaaacaggg gctggaagat gtttctggtc tgctggaact ggctgtagaa
    13861 gctgacgacg aagaaacctt taacgaagcc gttgctgaac tcgacgccct ggaagaaaaa
    13921 ctggcgcagc ttgagttccg ccgtatgttc tctggcgaat atgacagcgc cgactgctac
    13981 ctcgatattc aggcggggtc tggcggtacg gaagcacagg actgggcgag catgcttgag
    14041 cgtatgtatt tgcgctgggc agaatcgcgt ggtttcaaaa ctgaaatcat cgaagagtcg
    14101 gaaggtgaag tggcgggtat taaatccgtg acgatcaaaa tctccggcga ttacgcttac
    14161 ggctggctgc gtacagaaac tggcgttcac cgcctggtgc gtaagagccc gtttgactcc
    14221 ggcggtcgtc gccacacgtc gttcagctcc gcgtttgttt atccggaagt tgatgatgat
    14281 attgatatcg aaatcaaccc ggcggatctg cgcattgacg tttatcgcgc gtccggcgcg
    14341 ggcggtcagc acgttaaccg taccgaatct gcggtgcgta ttacccacat cccgaccggg
    14401 atcgtgaccc agtgccagaa cgaccgttcc cagcacaaga acaaagacca ggccatgaag
    14461 cagatgaaag cgaagcttta tgaactggag atgcagaaga aaaatgccga gaaacaggcg
    14521 atggaagata acaaatccga catcggctgg ggcagccaga ttcgttctta tgtccttgat
    14581 gactcccgca ttaaagatct gcgcaccggg gttgaaaccc gcaatacgca ggccgtgctg
    14641 gacggcagcc tggatcaatt tatcgaagca agtttgaaag cagggttatg aggaaccaac
    14701 atgtctgaac aacacgcaca gggcgctgac gcggtagtcg atcttaacaa tgaactgaaa
    14761 acgcgtcgtg agaagctggc gaacctgcgt gagcagggaa ttgccttccc gaacgatttc
    14821 cgtcgcgatc atacctctga ccaattgcac gcagaattcg acggtaaaga gaacgaagaa
    14881 cttgaagcgc tgaacatcga agtcgccgtt gctggccgca tgatgacccg ccgtattatg
    14941 ggtaaagcct cttttgttac cctgcaggac gttggcggtc gtattcagct gtacgttgcc
    15001 cgtgacgatc ttccggaagg cgtttacaac gagcagttca aaaaatggga cctcggcgac
    15061 atcctcggcg cgaaaggtaa actgttcaaa accaaaaccg gcgaactgtc tatccactgt
    15121 accgagttgc gtctgctgac caaagcactg cgtccgctgc cggataaatt ccacggcttg
    15181 caggatcagg aagcgcgcta tcgtcagcgt tatctcgatc tcatctccaa cgatgaatcc
    15241 cgcaacacct ttaaagtgcg ctcgcagatc ctctctggta ttcgccagtt catggtgaac
    15301 cgcggcttta tggaagttga aacgccgatg atgcaggtga tccctggcgg tgccgctgcg
    15361 cgtccgttta tcacccacca taacgcgctg gatctcgaca tgtacctgcg tatcgcgccg
    15421 gaactgtacc tcaagcgtct ggtggttggt ggcttcgagc gtgtattcga aatcaaccgt
    15481 aacttccgta acgaaggtat ttccgtacgt cataacccag agttcaccat gatggaactc
    15541 tatatggctt acgcagatta caaagatctg atcgagctga ccgaatcgct gttccgtact
    15601 ctggcacagg atattctcgg taagacggaa gtgacctacg gcgacgtgac gctggatttc
    15661 ggtaaaccgt tcgaaaaact gaccatgcgt gaagcgatta agaaatatcg cccggaaacc
    15721 gacatggcgg atctggacaa cttcgactct gcaaaagcga ttgttgaatc tatcggtatc
    15781 cacgttgaga agagctgggg tctgggccgt atcgttaccg agatcttcga agaagtggca
    15841 gaagcacatc tgattcagcc gaccttcatt actgaatatc cggcagaagt ttctccgctg
    15901 gcgcgtcgta acgacgttaa cccggaaatc acagaccgct ttgagttctt cattggtggt
    15961 cgtgaaatcg gtaacggctt tagcgagctg aatgatgcgg aagatcaggc gcaacgcttc
    16021 ctggatcagg ttgccgcgaa agatgcaggt gacgacgaag cgatgttcta cgacgaagat
    16081 tacgtcaccg cactggaaca tggcttaccg ccgacagcag gtctggggat tggtatcgac
    16141 cgtatggtaa tgctgttcac caacagccat accatccgcg acgttattct gttcccggcg
    16201 atgcgtccgg tgaaataagc attacgttat gcgcataacc ccggcagatg tcggggtttt
    16261 ttaattgtgc tgcgcgaaag cagacaacaa ttttcttgct tcactattgg ctgcctgcat
    16321 caccatccac ggactgaacg cccacggcgt ggcatcaata ccgtgtaata catctgctaa
    16381 atcacaccat tgataatcca tcacttcatc atcgttgatc tgtaacgcac tggtggtgcg
    16441 tgcggcaaat accggacaca cttcattttc cacaatgcca ttcggatcgg tggcgcgata
    16501 gcgaaagtca ggatagatag attcaggagg cgtaatttcc acgccaagct cataacggca
    16561 acggcggatc accgcgtctt cgttgctttc tcccagttgt gggtgcccac aaaccgagtt
    16621 agtccacacg ccaggccatg cttttttgct cagtgcgcgg cgggtaacta ataattgtcc
    16681 tttggcatta aacagccaac tggagaacgc gagatgtaag cgggtgtctg ccgtgtgtgc
    16741 ggcatacttt tccagcgtac ccgtgggaac tccctgtgca ttcaataaaa tgacgtgttc
    16801 cgtttgcata atttctcaca tgtaattctg atcactcatc attatagata attgaagagt
    16861 gaaatgatta accaagtgag cgtttatcga caaccgcccg ttttgagcgg atgccgacag
    16921 gtaaaaacca tttaaaaaag tagcatcata tatttgaagc gactttcaat ggccattgca
    16981 acacagtcag aaatgacagg aatagagaga gtaaaaagca acagcagact gaatatagcg
    17041 cgcattaaaa aattcatatt attctccatg ctcatttttc ttcagcttgc gcatcagaag
    17101 ccacatacgt actgtcctga cttttaaatc tttatcagag acggattcct tcaccaggtc
    17161 agcatgatgc tgatagccat taaagaagac gttcagcaca acggcgctaa gggttgcgag
    17221 cataatgccg ctatgtagca gcggttgcag tacggccggt aacttagaaa agaaatcgtg
    17281 agagagcgtt ggagtcatgc caacgccgag actgatcgcc acaatataga ggttgtaacg
    17341 gttggtggtg tagttacagc gcgacagaat tcgaatccct gtcgccagta ccatgccgaa
    17401 catcaccaga ccagcgccgc ccagcacaaa ttgcggaatg gaggctacca gcaccgccat
    17461 ttttggcacc atgccaaaca ggattaatat aattcccgaa gaaatacaca cccagcggct
    17521 atgaacgcgc gtcacgctaa ccaggccaac gttttgagaa aatgacgtgt gggggaagct
    17581 gttaaacgtg ccaccaatca ttgtccctac gccatcgaca cgcagcccgc gaataatatc
    17641 gtgcgaagag agtttacgac cgactatttc acccagtgcc aggaacatcc ccatcgactc
    17701 gataaacacg atgattaaca cggcagtcat ggtcagaatg gaaacgggat cgaaaatcgg
    17761 cataccaaac gacatcgggg tgacaatcgc aaaccacgag gcatcatgta gcccggataa
    17821 attgacttca ttcatcatcc acgaaagtaa aaagccaaat acaatcccca gtaatacggc
    17881 gacgttggac ataaatcctt tcgcatagcg agtaatgagc aagataaaaa ttaagacggc
    17941 aaaggagata cctaaataaa cgggattacc atattgcgga ttccctttac ctccggcggc
    18001 ccagtcaata cccacctgaa tgatgctaag cccgatagaa gtaataacca caccggtaac
    18061 cagtggcggg aataaaggca tcaagcgacc gataagttgc gctaataatg tggtgataaa
    18121 acccgcggcg atagtggcac caaatatacc cagcaggccg atatccgggt tcatcccaat
    18181 ggctatcatt ggtgttacag cagcaaaggt caccgacata atcaccggca ggcggatccc
    18241 cataaagcgg ccgataccga tacattgcaa taatgtgacg atcccgcagc aaaagagatc
    18301 cgagctaatg agcatcgcaa tagcttcttt tgagaggccc agtcggtcac caatcataag
    18361 aggaacagcg actgcacctg cgtacatgac aaggacgtgt tgcagaccga gtatgatcag
    18421 ctttcctggt gataatatgc ggtcaacctc atcagttggg cggtcttgcc cagaagatga
    18481 gggaagttgg gaatctatgg cgctcatcag tgtttctcct ttcgcccgcc agagtaaaaa
    18541 gcaaccgttt acgttaatga aactgtctaa acacatgctc gtggagtgcc agtttttgcg
    18601 aggaagggga gttagagaca ggaattgcgg aggcaatcac ataatcgtgc ttaaactacg
    18661 aaaatgacaa gagaacctga caatggaatt atctgtaatt ttctcatatt gatatttcat
    18721 taatcattct ggcatgtcgc actctcgcat ttaatcgttt ttatctggat agcgctattt
    18781 ggggcggcaa tgttgaaata atgaaagcgg catagattga ggggcaataa tttgaactca
    18841 gtcgcaaaat tataattaaa acggtcatgc tacctaatat atgcccgttt attgcatcgc
    18901 gaaatttgag aagacataac tcattaaatg aaagggatgc aaatgaataa gtttatcgct
    18961 gctgaagccg cggaatgtat aggctgtcat gcttgtgaaa ttgcctgtgc ggtggcacac
    19021 aatcaagaaa actggccgct gagtcacagt gactttcgac cacgtatcca cgttgtaggg
    19081 aaaggcctgg ctgcgaatcc ggtggcctgc catcactgca acaatgcccc ttgcgttacg
    19141 gcttgtccgg ttaatgctct gactttccag tccgatagcg tacaactgga cgagcaaaaa
    19201 tgtattggtt gtaaaagatg cgcaatcgct tgcccctttg gcgtcgttga gatggtcgat
    19261 acgattgcgc agaaatgcga cctttgtaac cagcgcagtt ccggcacgca agcctgtatt
    19321 gaagtttgcc caacacaggc gttacgactg atggacgata aagggttaca gcagataaag
    19381 gtggcccgcc agcgcaaaac ggcagcagga aaagcgtcat cagacgctca gccatctcgc
    19441 agtgcagcgt tgctccccgt taactcgcgt aaaggcgcag ataaaatttc agcaagtgaa
    19501 cggaaaaacc actttggcga aatctattgc gggctggatc cacaacaagc gacttatgag
    19561 agtgaccgct gtgtttattg tgccgaaaaa gctaactgca actggcattg cccgctgcat
    19621 aacgctattc cggattacat ccgtctggta caggagggaa agattattga agcggcagaa
    19681 ctttgccacc agaccagttc cttaccagaa atctgcggca gggtatgtcc acaggaccgt
    19741 ctttgtgaag gcgcatgtac tttgaaagat cactctggcg cagtctctat cggtaatctg
    19801 gaacgctaca tcaccgatac cgcgctggcg atgggctggc gtcccgatgt cagcaaagtt
    19861 gttccccgta tcgagaaagt ggcggtgatt ggcgctgggc ctgcagggtt agggtgtgct
    19921 gatattctgg cgcgcgcagg agttcaggtc gatgtctttg atcgccatcc agaaattggc
    19981 ggtatgctga cttttggcat tcctcctttc aaactcgata aaacggtatt aagccagcgg
    20041 cgagagatat tcaccgcaat gggaatcgac tttcatctta actgtgaaat tggccgcgat
    20101 attaccttta gcgatttaac ttctgaatat gatgcagttt tcatcggcgt ggggacttac
    20161 gggatgatgc gagcagatct gccgcatgaa gatgcgcccg gtgtcattca ggctctaccg
    20221 ttcctgactg cccatacccg ccagctcatg ggattgccgg agtctgaaga gtatccgctg
    20281 acggacgtgg aaggtaagcg agtcgtggta ttgggcggtg gcgatacgac aatggattgt
    20341 ttgcggactt ccattcgcct caatgctgcc agcgtgacct gcgcgtatcg tcgtgatgaa
    20401 gtcagtatgc cgggctcgcg caaagaggtg gtcaatgcgc gcgaggaagg tgtcgagttt
    20461 caattcaatg ttcagccgca atatatcgct tgtgacgaag atgggcgttt aactgcggtg
    20521 ggcctgattc gtaccgccat gggtgagccg gggccggatg gtcgccgtcg tcctcgtccg
    20581 gttgcgggtt cagagtttga attgcccgcc gatgttctca ttatggcctt tggtttccag
    20641 gcgcatgcca tgccgtggtt gcagggcagc ggaattaaac tcgataaatg gggcctgatt
    20701 caaaccggtg acgtcgggta tttacctacc cagacgcatc tgaaaaaagt ctttgctggt
    20761 ggtgatgcag ttcatggcgc ggatctggtt gtcactgcaa tggccgcagg aaggcaggcg
    20821 gcgcgcgata tgttaactct gtttgatacg aaggcatcgt gatgaaatcg ttaattatcg
    20881 ttaatccggc tgactgtatt ggctgccgta cttgtgaagt ggcctgtgtg gtcgctcacc
    20941 cttcataaca ggagttgaat gccgatatct ttttaccccg gctgaaggta cagcgactgg
    21001 actgcatcag tgcgccggtg atgtgccatc agtgtgaaaa cgccccttgt gttggcgctt
    21061 gccccgtggg ggcgctgacg atgggcgagc aggtggtgca gaccaattct gcccgttgta
    21121 ttggctgtca gagttgcgtc agtgcgtgcc cgtttggaat gatcaccatt cagtcgttgc
    21181 cgggggatac ccggcaacaa atcgtgaaat gcgatctctg tgaacagcgg gaagaggggc
    21241 ctgcctgcgt tgaatcttgc ccaacacagg cgctgcagtt gcttactgaa agagaactca
    21301 ggcgagtccg ccagcagcgt attgttgcca gcggtgagaa tcctctgtga aagaaatctc
    21361 cgcgctgaaa ggcgcggaga atctattaga ttgcccaacc acccgcgtaa aatgcgacca
    21421 gtgcggcagt aatgatcacc gtaccaatat tcagcttttg ccattcgcgt gcaaagacgc
    21481 ggcctacgac cagtgtcaca aagcccagca taataccggt aacgatatta caagtcagaa
    21541 cgatgaacac ggcacacacc aggccagcca tagcgtcaat aaaatcattg aaatccagct
    21601 tcgagacgtt acttaacatc agcaaaccta cgtacatcag agcgggtgca gtggcgtaac
    21661 cagggatcaa aaatgacagc ggtgataaga acagaatcag caggaataac gcccccacta
    21721 cggttgcggt taaccctgtt ttacccccgg cggcggttcc tgccgctgat tcgatataaa
    21781 ccgctgcggg cgctgcgccc accaggccgg agaatattga acttactgag tcactggtca
    21841 gggctttgcc gccgttgatg atctggttgt ctttatccaa caaattcgcc tgaccggcga
    21901 cggcacggat ggtgccagta gcgtcgaaca ctgcggtcat caccaatgcc agtacactcg
    21961 gaagtacagt tggctggagt gcgcccataa tatcgaggct gaaaatcaga gacttaccat
    22021 cttcgccagt caggcttggc atcgccacca gaccgtggta tttcaccgct gggtcaaaga
    22081 ttaagccgat gatcgaaatt gcaataatca ccaacaagat accgccgggt acgcgacact
    22141 tctccagacc gaagatgacc gccagcccca gcaagctcat catcaccggg aaggaggtaa
    22201 acgcaccgag cgccactggc aagccttcaa tcgggttttt gataaccata cccacaccgt
    22261 tagcagcaat cagcagcaga aacagcccga taccgatacc tgtaccgtga gcgataccca
    22321 tcggcaaatt acgtaagatc caggtacgca caccggttac ggaaatggcg gtgaagatga
    22381 cgcccatcag aaataccgcg cccagtgcga caggaacgct aatttgttgc ccgagtacca
    22441 gactgaatgc ggtaaacgcc gtcaaggaaa tcgcgcaacc aatcgccatt ggcaaattag
    22501 cccataaccc catcagcaac gagccgaagc ccgcgaccag acaggtggca acaaacacag
    22561 ctgcgggagg aaaacctgct ttgcccagca ttcccggaac gacgataacg gaataaacca
    22621 tggccagaaa ggtcgttaag ccagccagta cttcctgacg aacggtactg ccacgagcgg
    22681 taattttaaa ataattatcc agcgcgccct gtggctttgg tgcgtccggt gtttgtagga
    22741 tgtctccaga cataatgatg tcctcacgag gatgtaaaag ttttattatt agttgcgttc
    22801 gtacaccaga cgaccatcaa cgtaggtgcg gtagatcgaa cggtcatcgc ccaacgtcat
    22861 catcacgaac aatttgtcga ctaaagaaac agagttgtca tagcgcagct gctgtagcgg
    22921 agtggcggtg ggttccatca ccacgaaatc agcctctttg ccaggtaaaa agttgccaat
    22981 caaatcgtca aggcccagag atttcgctcc gccgagcgtg gccaggtaaa acgcttcata
    23041 tgccgagagg cgatagcctt gtaattgcaa cactttgtag gcttcgttca gcgtttgcag
    23101 catgttgaaa gtggttccgg caccgatatc cgttcccatg cccactttaa ctttcttctg
    23161 ccatgctttt ttcaagttga ataacccgct gccgaggtaa aggttggagg tcggacagaa
    23221 agcaatgctg gatttggttt cgctgagacg atcccactct ttttctttga gatggacgca
    23281 gtgagcaaag acacagtttt taccggtcag gccgtactga tggtaaacat ccagataacc
    23341 atcatggtca ggataaagcg atttcaccca ggcaatttca tctttgtttt cacagagatg
    23401 ggtatgtacc cacgtatccg gatattcctc tttcaggcgt tgcgccatcg ccatctgttc
    23461 aggagaagaa gtaggggcga agcgtggcgt aatcgcatat agcagacgac catttttgtg
    23521 ccagcgttca atcagttctt tgctttggtg atagctgctt tcggcagtgt ctagcagata
    23581 atccggtgcg ttgcggtcca tcatcacctt accggcaatc atacgcatat tgatatgact
    23641 ggcggcttca aacagcgcat caacggattg cggatgaaca gtgccaaaca ccagcgccgt
    23701 ggtggttccg ttacgtaaaa gctgcttgat gaagaacgcc gacatttcgc gggcgtactc
    23761 taaatcctca taacgacgtt cagtagggaa ggtgtgttta ttcaaccact ccagcaattg
    23821 ttccccatag gcccccacca tttcactttg cggataatgg atatgtgtat cgacaaagcc
    23881 cggtactatc agtttgccgc gatagtcgcg cacgcgaata gtgtcaggaa tttgatgctt
    23941 tccgtcttcc cattcgccaa accattccac ttttccctgt ttaatgagta ataaaccatc
    24001 ctcaataaac cgcagcgcag aggcaatctc ttccgggtta tcgaccgtac gggtgacatc
    24061 aataaaactg cctcgtaccg cttttaacgt gtgttctcct gacatcattg actcctttac
    24121 tgttaatcca tctcttccgc tgaggtaata ccaggcagaa cgttttcctg tcggtagcca
    24181 ccagggagga taatatttaa taaaatcgca gttaacccgc cagcacaaat agggttttca
    24241 actaatacat aaatagaggc tggcaatatt ttaaaaattt cgggatcgta ggagacgcca
    24301 agtcctaaac ctaaagaagt ggcgacaata agtgtttctc ggcgctttaa accgttggtg
    24361 atgatgatgc gaatcccagc gatggcaatc atggaaaaca tcaacgtcat tgcgcctccc
    24421 agaactgccg agggaatggt cgtgaagaag ccgccaatca tcggaaacaa accgaggata
    24481 accagcatta ccgcgatggt tcgcccgaca taacgtgaag cgacgccagt catctgaata
    24541 accccattat tttgcgcaaa cgtggttaag ggtaatgacc cgacagcgga ggcgataaca
    24601 gaaaccagac cgtctgccag cacgccgcct ttcagtcggg actgatactc ttctccctga
    24661 atgggtcggc gggaaaccat tgccgtggcg gtgatatcgc cgacagcttc cagcacgcta
    24721 agcagataaa tcgtgccaac caccaggaac tgatggaagc taaaactaaa gccgtatttg
    24781 aacggatgtg ggatggtgat taacggcaaa ttgcgcatac tgctgaaatc caccatgccc
    24841 aggcataacg acgcgatata accgacgcat agcccaatgg cgatccctcc catgcgtagc
    24901 aacggactgc gacagcagtt aaagccgatc accacaatca aaaccaataa accaacgccg
    24961 agatgttcgt aattgccgaa ggtaccgctg cttttggcag caaatcctcc accaaaatcg
    25021 ataatgccga ctttaatcag gcttaagccg atcatcagta ccacaatacc gctgacagta
    25081 ggcgtaataa cccgacgtaa atagggcaag ataaatgaag atccgacaac cagaaatgcg
    25141 ccaacgaagg agacgccgag gagcgacgac atgattaact cttcgtgaaa accgtcgctt
    25201 ttcatgctgc tgcccagcgc aatcatgacc gtaacaaatg aaaaattgac tgactgaatt
    25261 gagagtaggc cagaaccgac gatgccatag cggtttactt gtaaccaggt accaatacca
    25321 gaggcgatca tcgccattga aacaagatag gcagttgttt cagcggaaag ctgtaaggcc
    25381 gcccccacga ttaacgcggg ggttaccatc ggaacgaaaa ttgccaacag atgggtaatg
    25441 gcaccaacga gagcctgatg aaagggaggg cgatcctcca gttcaaatat aaggtcagaa
    25501 cctgcatggt ttatatcaga catcccttca ctccttttaa agtaaatttg atcatctgaa
    25561 ttagtaaaat aaattcccac gaatttgggg cgcaggacgc cttgagaacg gcttatctca
    25621 ttttcgcagt cactatttag tcatcgtcca aacagagaaa caggccgccg aagcggccat
    25681 ttattattta tatcttttcc agcgcggtga gtattttctc cggtgtgaaa tgccattcgc
    25741 gtaaccagat gccgcatgca tcgtgaattg cggtagcaat cgccggagct gcgccattta
    25801 caccgatttc cgagatcgat ttcgccccga acgggccgac tttatcgtcg ctcggcacca
    25861 gcactgcgcg gaaatcacgc ggaatgtcac caattttcgg tgcgccgtag ctgcgtaaat
    25921 cacgcgttaa cgggtgacct tcggcgtcat aaatgatctc ttcgctcata ctgtggccga
    25981 tagctcgcag ggtagcaccg tagatttgtc ccaacgctaa ctctggattg accggtgtac
    26041 cgcagtccag caaggcgtag aatttatcca ggcggatttc acccgtacgc gtgttgacgg
    26101 caacttcagc gaagtttgcg ccatacggga aggcgaaatc aggcgtgata taactgccag
    26161 ttcccaccag tgaaccaaag ccggtgccgg tttcgccttt atgggcaata tccccgaaac
    26221 taacttcgcc tttcttgccg cgcacgacgc ccggcgttgc tagttgaaca tctgccactg
    26281 gctcacccaa catttgcgcg ccgtggaaca gaattttctc ccgcagattt tccgctgcca
    26341 aacgcgccgc gttacccgag aagcaagtac cggacgaggc atatgcgcct ttatcaaaca
    26401 acgcatgatc ggtatcaccg gagataacat gcacgtcctg cggtgggcag tgcagcactt
    26461 ctgctgccag tttcgtcacc acggtatcca gaccagtacc aatatccgca ccgccagaat
    26521 gaacgataaa ggtaccgtct gattccagtt tgatcatgca gttagcctga tcgatatccg
    26581 ggatccctga tttctgcatg ataatggcga caccacgacc gatgtgccag tcaccatttt
    26641 gtggttttgg tgaagaccat tggatcatct cgcgcccctg acgcaggatc tcttccagtg
    26701 cgcagctggc ggcggaagga acggaggtcg gcgctttacc ttcaccgatt gcaccgagaa
    26761 ttttcagctc ttgcccttcg tgtacccggt tacgttcgat aatttccagt tggtcgatct
    26821 gtaactgttc agccagttcc gctaatgcca tggtgatagc gaagttacct ttcggtgcgc
    26881 cataaccctg ataagcacca tttgggcaaa tgttgctgta gtaggtggtg acctggaaat
    26941 cgacgttatc gcacggatat aacggcagcg acagcgccgg tccgttacac ggtacggtga
    27001 gtgagtggtt gccgtaaggg ccagtgttgg cgcggaaatc catcttcact gccgtcaggc
    27061 gaccatcttt tttcgctccc agtttgacgg tgactttcgc gacgtgacga gaggtgttag
    27121 caataaactc ttcttcacgg gtgtagcgga acagtaccgg acgcccggtc acgcaggttg
    27181 cccaggcgca cacttcttcc agcaggatgt cctgtttgga accaaaaccg ccgccaactc
    27241 gctctttaat gacatgaact ttatgctgtt tcatgtccac gaggcgcgcg acctggcggc
    27301 gtaagtgcca tggtacctgg gtggaggcgt ggataaccag acgatcgccg tccatccggg
    27361 taaagcagat atgtgtttca gtcgggcact gctgcgcctg cgttgagtta taggttcgct
    27421 caatgatcac atcggcatcg gcaaagcctt tgtccatatc gccaatatga ccatgaatac
    27481 tagcggcgat atttttgcga gggcgagaac cgatcgggaa gttgatgatc atatgctcgc
    27541 cgcgctgggc tgcatggctg ttatcgtctt ccagagtatc tggcgcacca gcaacataca
    27601 ccaccggttc atcgtgcacg acaggcgcat cttccgccat tgcttcgtcg atcgacatta
    27661 ccggcttaag cacttcatat tcaacctcga tgagtttcaa tgcttcgagc gcaatttctt
    27721 cactttcagc gaccaccgca gcaacgcgat cgccgacgtg acgcattttt ttgccgaaca
    27781 tacggcggtc aagcggtgac ggttccggtg cgctctgacc ccccggggta tagtagatat
    27841 cagggcaatt caggtgagta ataacgtgaa cgacgcccgg taaggcttca gctttgctga
    27901 catccagatg agtaatcagt gcgtgagcgt gtgggctacg taacatttta atgacgcagg
    27961 cgtcagccgt tacgcggtct tcaacatagc agggtttcgc ctgcaccatt ttcgcggcat
    28021 cagttttagg ataatgcttg ccaatgactt caaggtcgtc acggaaagtc ggagcgatat
    28081 caatggtggc ctgcggatta tttttacgtg caaccgccag ttcaatgacc tgatagtatt
    28141 gctgccagcc agcatcgcgg ctgaacaaac cagaaagcgc gtcgtcgatc tcttcgcggg
    28201 taggtgcggc gatgcgatcg agaagatcgg tgataatcag agctgcagct ggatcgttat
    28261 aaccagactg caccacgcca acatcaacca ttgcctgttg aaccagactt aactcgttcc
    28321 atttgcccag agattctgcg gtacgaatat ctgccttctc taactgtgcg gcaataagca
    28381 aggacgcgtt aacgatatta ccgttaaaga tgattgcgtc agaaccggca aacccgaaac
    28441 catcatcact gttgcgtaca gagtgcattc ccatgttaaa caacagcttt tgcacgtttt
    28501 cgcctggatt aacggttagc tcctgaggcg cgccatttaa agtaaagtgg atgatcatac
    28561 ggcttcctcc ccagcctgtt ggcagtcggc atacaggtcg gctaccagaa ctcccgtgat
    28621 atagcgttta taggccacgc tgccccgcaa atcttcctgc gggaaaatgg cgttggcgac
    28681 agcctgttca agtgcattgc cttccagatt ttgtttttcg acatcatgca gacgcagtgc
    28741 tttactggcg acgccatcca gcgcaattcg cataccgtcg tggtctgtca ttgcaacggc
    28801 tgccgtcacg acggttaaac ctgcctgaga acggctaatt ttgcgggtcg cacaggtgcg
    28861 atacggatct ttaatgataa tttcggttag caggcgatcg catgggcagg ccaggtagtc
    28921 ctcgattgac agcgtttcgc cgttgccaaa aaccagttca gcatccagtg ccagcaggac
    28981 gggaagcagc actgactctt cctgccgggc ggcgatttca ccaccaatgg tcgactgatt
    29041 acgaacatgg cgtgagtaaa caaaaccgag ggcttcacgc agtgctgcag gaataaatcg
    29101 cgcatcacgc agtggctgca agcgagacat tgcgccaatc cgcagtgcac cgttatccca
    29161 gtcaacccag tccagttcca gatcctgtaa ggaaatggca atctttttat cggtacgggt
    29221 tggtgtagca ttgagtttgc tgcccccggc gaaccagacg gcttcatcct ggtagcggcg
    29281 cttcagttcc agcgcctgtt cgacagagtc gggcctgaaa aattgttcaa tcatcttctt
    29341 tccttattta accctcttcc tgcgggaaga gggcatctgt catttatgcc agcgcatcca
    29401 tccgacgcca catactggca gcggcttttc tggcttgtgc ataaatggaa tcgcaatcga
    29461 agttaaactg acggtcttca tagaccatca caccattgac catcacgctg tgaacgctgc
    29521 ctgagcccat accgaaagcg atatgcccgg cgatattgtc tgccagcagc ggcgtcggcg
    29581 agttgtaatc gcaaatggtt aaatcagctt tgtaaccggc ttccagaagc ccaaatttcg
    29641 cgccaaagtt gcggctcatc agttcgttac cgttagtcag ggctttggca aaactgtcag
    29701 gccacagcgg accacccgca tcgcgatgtt taaagaaggc aaatttcatc tcttcaaaca
    29761 tgtccgaacc aatgccgtcc gttcccaacg ccagattgcg gatgtcgcta aggtgatggt
    29821 tgtagccgac atggttgttc atgtttgaac gggcgttatg caccaggaac gcatcgcgct
    29881 gattgagtag ggcgatgtca tctttcgaca agtacagccc atgagcgacc agcgttttgc
    29941 tatcgatgag atcgaattgc gccagtcgtg ccagcaggtc tttgccgtac cagtggtgac
    30001 tgtaggaaac gtcgtaaagg tcttccgcag cgtgaatatg caaaccacgg cctgtggcgt
    30061 tcacggcttc acgtagcatc tccagaccgg catctggcac ggtaaacggc gcgtgagcac
    30121 cgatatgtgc ttccaccaga tacggctcgg ttgctgcttt cttcgcctca tcaatctgac
    30181 gggcgaagcg gatgttttct tctacacctt cctgcaactc tttgatgccg ttgttacggt
    30241 cagtagtttc aaaacaggtc atcgcgcgca ggccaacttt taaaaatgcg tcgcgcaatg
    30301 tggagagcga cccgccgata tacgccggag aggcatggtg atcgataacc gatgtacatc
    30361 cgctcttaat cgcttccagg gaacaaatca gcccgctgta atagagcgac tcttcatcaa
    30421 gggcgcgatc gagccgccac cagagatttt tcagcgttga gatgaaatct gggctggggg
    30481 cgatgtttgc cataattccg cgggaaagcc ccgagtaaaa atggttgtgc gagcagacaa
    30541 ttcccggcat cacaatccga ccatgcatct ctttgtagct ggcatcgggg tagcgttgcg
    30601 tcagggcatc gccgatagcg acaatcacat cattttcgat agcgatatca acgccttcct
    30661 gcactttcgc cgggtgtaac tgcactgcgg tgacattctt cagaatcaac atgattacac
    30721 ctccacgcgg cccagcagat aatgatgatg ctggtggaca tggctgatga tgcggcacat
    30781 atcgttcagc tccggtggta cgttgttgaa ctgaccttcg ctgtcgatgt ttaacaccca
    30841 gctttggtta ttcagtcgta ctcgtacccg gcaatcttcc acaaggaagc ctgggttgct
    30901 gctgttatca aagtcttgcg ccaggctgaa aacggtgatt ttgtctttgt atggtttacc
    30961 gttccacgga cagaactgag cgcagttgcc gcattcgtta cagtaagcgt cgaggtgcag
    31021 cgtctggaat ctattctgga agcctgggac cgcaatggag acgttggcgc ggttcgggca
    31081 gacatccaca cacttgctgc aaacgtagtt acattcaagg cagcgagcag cttcctgcgc
    31141 gacaaacgca tcacgatcgt cactgttcac cagcgtgata gagatatcgc ctttacgttg
    31201 atagatttcc gccggattga cgttgttcca gtatttatcg ttctggtggg aacggatatt
    31261 ttcccgacta aggatggcat cggtcgcccg acgcgcggtt ccgacagcag cgacaatgga
    31321 ggatggtccg cgctgtacgt cgccgatcat aaagacgtca gtcagacgag tttcgccatt
    31381 atggtcgacg tctggccagc cgtttttgtc cagcggcacg cccatcgcat tcagggcttc
    31441 agtatcctgc tgttcaccaa tggcggtgat caggctgtct acatgcagtg ttactgtttc
    31501 gttggtttca accggacgac gacgaccttt ctcatcgggt tcgccaagcg acataacgcg
    31561 caaggttaag gtgccatcag catcgaaacg ttccggatta ttcaggaaac ggaactccac
    31621 gccgtcgtgc aacgcttctt catactcttc gcgccatgcg ggcatctctt gcagtgaacg
    31681 acggtaaacg accgttgctt tttctacgcc tggaacgcgt aacgccgcac gagcgcagtc
    31741 cattgcggtg ttacccgccc cgacaacgac cacatgtttg cccagcttga gcgctgtacc
    31801 cttgttgtat tcacgcagga aggggagtga tttcaagaca ttttggttgt cgcccgccag
    31861 tttcacaccg ctatttttat ctgtgccggt ggcaatcaga acatagtgga agtcctgatt
    31921 ttttaactgc tcaacggtta aatcgggtga gcagccatac tcaaatttca cgccgtgagc
    31981 ggcaacaaaa tcgatatcgt gctgaattaa ctcagccgga attcggaact gaggaatgat
    32041 atttttcacc acgccgcccg cattggcttc gcgttcaaac agcgtaaccg gatggcccgc
    32101 tctggcaagg aagtaacctg ctgccagacc cgccggacct gcaccaatca cggcaaccgg
    32161 atggcgtgaa ccagaaccgg ctggtttgtg ccagcgttgc ttatattcat cccaaccttt
    32221 ttccagcgcg acttttttca gttcgcggat attcagcgca ctgtcgtaat ccaggcgggt
    32281 acagttgtat tggcactggt gatcgcaaat atgaccggta atggcgggca gagcgttgcg
    32341 ttggtagatg agttccagcg cgtcggcata gcggtgttcg ccaagcagac ggatgtattc
    32401 cggaatatct tgcttgatag cgcaggcagt aacacagggg gcaacgtagc agtcggtcag
    32461 cggcaggtct tctgccactt caatacgctc ttctggcttc cagtgtttct gggtgtattc
    32521 catggttaac gcatctgctg ccagtctgtt cagtcgttcg acgtcaacat ggtcaagtcc
    32581 ccaggcgtcg gagccttcca gctcgcgcat gcaggcactt aagcgcaaat agccgccagg
    32641 tttcagcagg tcggttgcca tagtaatagg gcgaatacct gtatcaaaaa tatcgcggat
    32701 agtcagctga ctggcaccac cggaataaga aatgggcagt ttgccgtcaa aggcgcgaga
    32761 gagaactgct gcaacattga tggagagcgg gaacagcgca cggcctgaca tatacatctc
    32821 ttcaccaggc agtgcgcctt tattgttgat ggtgccgaga gtgttagtca gttttacgcc
    32881 aaagccgagt gatttttctt ttgccagtgc catcaggcgt tccagcattt ccagtgcttg
    32941 cgtcagcttg aggtcgtgat caaatgactc ttcttttaag cctatgtaac cgaaaccgca
    33001 gacatcgagg atctcacgaa cacgcgcgta ccccagtaag gtcgggttaa gtttcacaaa
    33061 ggtgttgagc cctttttctt ccagcatgta gcggcaaatg gcttcgattt catgcggagg
    33121 acagccgtgc atggtggaga gggtgacgcc atgcaccata ctggtgggga tgcgagcggg
    33181 taaggcttgc aagctttcgc gtttttcctg caatccgtgg cgggctaaaa atgcgtcatc
    33241 ctggagtaat ttattcagcg tatcgcgata ttgcgcgaat ttcggatggt cagatgcgtc
    33301 catcatattg tcgatgaact gttgcattgg cggttgctta ataccttcga ggttgtaacc
    33361 gacgctcata ttaaagatga acgatttacc ggaatcagaa ggttggaaca tcgcttcgag
    33421 aaggtgcagg gcaaaccagg ctttgaggta ttcatcccag gctttaagca gggtaaattc
    33481 ggtagaccat tcggtgttaa agcactcgtc ttcggcatca atacagggct tttccagctc
    33541 caggcggtca agaatttgga cggtttttag ttcgatgaaa cgtccgccag tcagccagga
    33601 agtgacaata ttttgcgcga gctgcgtgtg cgggccagcg gcagggccga cgggagtggc
    33661 acaggtttca ccgaagacgc taacagtttt accttttaca ggtgagtaaa actgttgctc
    33721 gggaatacca aagattgagc gttgttgttg gtattcatca aatatgcgcg tcaaaagttc
    33781 ctcaaacgga atgggacgca taatatcccc cataaccctc tccttagctt atacagatat
    33841 cattgagtga ccggtgttga gaaatccggt ggtttagggg gatagagcaa caatcgcacc
    33901 actcttgcca ggtttggcgg gaaatataaa aaaatgtgag gcggctcgaa acttaagggc
    33961 tttttgtgaa tgaactcaca ggtgctaaat gagaattaaa atcactcctg aaaattatta
    34021 ttgccatccc atatatcact ttttctcttt tctcaaaatc agtgattcct tatcattatg
    34081 ataaagagtg ctggagggat taaatatttg aacgcaatcg tttaactacg ttttttaaat
    34141 tttagtatac cttttttgcg ctgtaataaa gtcgtccgga gtatcaatat ctaaaattat
    34201 ttctgcattt tctacttcga cggaataatg agctcccata aggagtgcct gacgcatatt
    34261 ggtgatattg gggcgcttga ttgcctgcat caggcatggt tttgacacta aaatcggatg
    34321 gccggggatg ccattatgga gtggcagtat tgcgccatca tttcgtaacg accagatttt
    34381 tctgaaaata tcgatggtga gggttggcat gtcaccgtgg gtgagaaaac aatgttctgt
    34441 ttgcactgcg ggtactgcgg cctttactga tgttagtaaa ccctgcgcat aatctgggtt
    34501 gtggataata gtgatattgc tctggttcgc ataacgttcg tgcagttcat taccacgata
    34561 gccggtgact aaaataattc ggctacaaaa ctgcaacgca tttttgatac ttgtatcaag
    34621 aattgtcccc tgttgccagg gtaacatcat tttccattgc cccattcttg atgataatcc
    34681 ggcagcggta attatacagt cgatggctga catagtggtt ccattattta taggggctaa
    34741 taacgcctga taattgcgtt acttcctgat ggattaagtt ggggataatt tatcccagag
    34801 aggtcataaa gactcgtgaa aagtatagtt gacccatcgg cattattcat tgatttaggt
    34861 gcgcagaaac gtcccactgt gatttctgtt gttggtgctg gggggaaaac cagcttgctt
    34921 ttttggctgg cagaactgct ccaggctagc ggcaggcgtg tgttaatcac gacgacaaca
    34981 catatgttta tgccaacgtc tcactggccc gtggttttct gtcgtgatcc cgccatgctt
    35041 cctcatgcgt ctcttacatc ccccatttca ttttgttttc acagctggaa agcgaacctg
    35101 ggaaaagtgc agggatttac gccagaagcg attgatgcac tggtgcaacg accagagtgt
    35161 gacgtaattc tcattgaagc agatggctcg cgtgcaatgc cgttaaaagc gcctgatgag
    35221 cacgaacctt gcatacccaa aagcagttgc tgcgtgattg ctgtgatggg aggacatatt
    35281 ttgggcgcga aagtgagcac ggaaaatgtc catcgctggt cgcagtttgc tgatattacc
    35341 gggttaacac ctgatgcaac cttgcaactg agcgatctcg ttgcgctggt tcgccaccct
    35401 cagggggcgt ttaaaaacgt accgcaaggt tgtcggcgag tctggttcat taaccgtttt
    35461 tctcaatgtg agaatgcgat tgcgcaaagc gagctccttc aaccgctgca acaacacgac
    35521 gtagaggcaa tctggctggg cgatatacaa gaacatcctg caatcgcgcg cagatttgtg
    35581 aattagcggc ccgggtattc cgggttactg aaaggatatc cattgaggtt ggtatgaata
    35641 ttttcacaga ggctgcaaaa ctcgaagagc aaaattgtcc gtttgctatg gcgcaaatta
    35701 ttgatagccg aggctcgact ccccgccatt ctgcacaaat gttagtgcgc gccgatggct
    35761 ctatcgtcgg tacaattggt ggcggaatgg ttgagcggaa ggtgattgaa gagtcgcttc
    35821 aggcattgca ggaacgtaag ccgcgattat tccatggacg tatggctcgt aacggtgcgg
    35881 atgctgtcgg gtcagattgt ggaggtgcga tgtcagtgtt tatcagcgtc catggtatgc
    35941 gtccacgtct ggtgttgatt ggcgcggggc atgtcaaccg ggcgatagcc cagagtgcgg
    36001 cgctattagg atttgatatt gccgttgccg atatttatcg cgaaagcctc aatcctgaac
    36061 tattccctcc atcaaccacg cttctccatg ctgagtcgtt tggtgcggca gtggaagcac
    36121 tggatattcg ccctgataat tttgtcctga ttgccacgaa taatcaggat cgtgaagccc
    36181 tcgacaaact cattgaacag cccattgcat ggttggggtt gctggcaagt cgtcgcaagg
    36241 ttcagctttt cctgcgtcaa ttgcgtgaga aaggcgtggc tgaagaacat attgcccgtt
    36301 tacatgcgcc cgttggttac aacataggtg cggaaacgcc gcaggagatc gccatcagcg
    36361 tgctggcaga aatattacag gtgaaaaata acgcgccggg tgggctgatg atgaaacctt
    36421 ctcatccttc cggacaccag ctggtggtga ttcgcggtgc gggagatatc gccagtggtg
    36481 tggcgctacg tctgtatcat gcgggtttta aagtgatcat gctggaagtg gaaaaaccga
    36541 cagtgattcg ttgtaccgtg gcgtttgccc aggccgtgtt cgatggcgaa atgacggtcg
    36601 aaggcgtcac tgctcgcctg gcaaccagct ctgcggaagc gatgaaactt accgaacgcg
    36661 gattcatccc tgtgatggta gatcccgcct gttcattgct tgatgaactg aaaccgcttt
    36721 gcgtggtgga cgctattctg gcgaaacaga atttgggaac acgggcagat atggcaccag
    36781 taacaatcgc gcttgggccg ggctttgctg cagggaagga ttgtcatgcg gtaattgaaa
    36841 caaatcgcgg gcactggctc ggtcaggtga tttactgtgg ttgtgcgcag gagaataccg
    36901 gtgttcctgg caatattatg gggcatacca cccgacgggt gatccgtgct cctgctgcag
    36961 gcattatgcg atccaacgtg aaattaggcg atctggtgaa agagggcgat gtgattgcct
    37021 ggattggtga gcatgaaatt aaagcaccgt tgacggggat ggtgcgtggc ttgttgaacg
    37081 acggcctggc agtggttggt ggttttaaaa ttggtgatat cgatcctcgt ggtgaaaccg
    37141 ctgatttcac cagcgtttct gataaagccc gggcgattgg cggcggcgta cttgaggcgt
    37201 taatgatgtt gatgcatcag ggcgtgaaag cgacaaaaga agtgctggaa gtggcttaaa
    37261 tgaaaatggc cggatggtat tacccacccg gccttaaaat tactgacaaa tcaccgttcc
    37321 ggttttgcct tcaatccctt ctttcgcttt gctcaatacg gtaatcagtg cttcacgacc
    37381 tgcgcgagag cgagcaaacg aggcggcagc ttccacttta ggcagcatag aacctttcgc
    37441 aaaatgacct tcttcgataa agcgttcagc atcacttaat gacaggcgat cgagccactg
    37501 ttcattctct ttaccgaagt taatggccac tttttctacc gcagtcagaa tgatcagcat
    37561 atcggcatca atcatttctg ctaaacgggc gctggcccag tctttatcga taaccgcgct
    37621 ggcaccacgc agatggttac cttcacgaat aacaggaata ccaccaccgc caacggtgat
    37681 caccacctgg cctgcttcta ccagcgcttt taccgtctct ttttcaatga tatcaaccgg
    37741 ttttggcgag gcaacaacac ggcgatagcc gcgaccagca tcttctttca gagtgtaacc
    37801 ctgttttgtc agttgctctg cttcttgctc ggtaaagaac gaaccgatcg gcttggtcgg
    37861 gttgaggaat gccggatcgt tagcgtcgac ttcgacctga gtaaccagcg ttactacagg
    37921 tttattaatg ccccgagaaa gcagttcttc tcttaaggcg ttttgtagat cgtaaccaat
    37981 ataaccctgg cttaaggcaa cacagacaga catcggcagc attggcgaat gcgcttcagt
    38041 ttttgcggca gcttcaaagg cctggttaat catgcccacc tgcggcccgt taccatgagt
    38101 gacgataact tcatgtccct gggcaattaa atcaacaatt gcctgagaag taatttttac
    38161 cgctttcatt tgtccggcca ggtcatcgcc cagcgcattc ccgcccaggg cgagaacaat
    38221 tttcttactc atttttcctc actcatcatt cttgattgtg tatagatccc tggcagtggt
    38281 tagagcacgg gagggacaaa cggtttgcgt cgcaggaaac gacctcgccc ggctttgcct
    38341 gtaaaggtgc cgtcacagaa aatcgtttca ccacgggata aggttctgac aatcgcgccc
    38401 tgacaggtaa aaccctccca tggcgagtag tcggcgttgt cgtggagatg gcgatgctga
    38461 atttgttggc tctgacgtgg gtcgataatc accacgtcgc catcggaacc gggcgctaat
    38521 aatccttttt gcggccacag gccaaacagc ctggcgggca ttgcgctggt taattcaaca
    38581 aagcgttccg gtgttatacg tcccgtcatc acgccactgg aaaacagtaa ctgcatgcgg
    38641 ttttccacac cgggtaagcc atttgggcag cgactgaaat cgcctttaga aatttgcagg
    38701 cgttgagcca tcgagaaggt gcagtgatcg gtcgccacca cgtcaatcgc accatcgctg
    38761 atgccacacc acagtttgtc ctgctcgcgt acgttacgca gcggtgggct aagaatgaac
    38821 ttcatgccgt cttctgtatc gtaactgcgt tcgtccaaca ggagatattg tgggcaggtt
    38881 tcaacccaga ctggctggtg attcgcacgg gcaagacgca gataatccag acctaagccg
    38941 ttagacaggt gcacgatata gagcggggcg ttaccggcaa tttgtgccag gttaatcatg
    39001 cgggcgatgg cttccgcttc gcattccaga gggcgactca aggcatgata gcgcggcgcg
    39061 gttaaccctg cggcgataaa ttccgcccgc ttgctggcga tagccgcatc attttccggg
    39121 tgcacggtgg tcagcgcgcc ggattcatgc agacggcgta atgcctgcaa aacctcgtca
    39181 tcgttgagtt tgtattgata ggttaaatag agtttaaaac tgctcagccc ttcctcgacc
    39241 atcatcggga tttcgtcgag gatggcgtga ttaatgtgct ggatcacacc gtgaaagctg
    39301 taatcgatga ccgctttatg ggcggcataa ccacgataaa cctccagttg atggcgtaac
    39361 cgacagccgt ttgggccaaa tcccatatgg tcaataatgg ttgttgtacc gccacacgca
    39421 gctgcgcggg taccggtaaa aaaatcatca caactgcgcg cgatgccgac atcaatattg
    39481 aaatgcgtat ggacatccac gccgccaggg aaaacgtaac agccagtggc atcaatttct
    39541 tcatacggga gctgcggcga aatattgttg cccaactggc gaacaatccc gctttcaatc
    39601 agcaaatcct gtttggcttg tccatctgcg ttaacgacag tgccgttttt gatcaatacg
    39661 cgcatagcaa actccaaaaa ccctccggcg aaccggaggg gaataagtga agagtaatta
    39721 ttcggttgcc agccagctta acgggattgc agcgtacatc gcggcacagg tcaccaggtg
    39781 agatttccag gttttttcgt taggtgcatg cgcttcaggt tctttacccg ggccaaagcc
    39841 gatcaccgga atgccgtgac ggcccatgat agatacgccg ttagttgaga aggtccactt
    39901 atcaacaacc ggcgctttgc caaacagccc ttcgtaggca ttcaccagtg ctttaacggt
    39961 gaagtgatct tcttccactt tccaggtcgg gaagtagcat tcggttgggt aaaccaggcc
    40021 agtccaggac ggacggtcgt agttgtacat agaaacaacc gcgttagctt tctgtactgc
    40081 aggcagggcg cggatttcgt ccagcgcgcc ttcccaggtt tcgccccagg tcagacggcg
    40141 gtcaatagag acggcgcagc tatctgctac agcgcaacgg cttggggatg tgaagaagat
    40201 ttcagaaacg gtgagggtgc ctttgccgag gaattcgtca taacccagac gttgggagag
    40261 ttcttgtaat tcgccaagaa tcggacccat tttgaaaatg gcgttgtcac cgcgttctgg
    40321 cgcagaaccg tggcagctaa caccctgaac atcaatacga atttccatac gaccgcgctg
    40381 accacggtat acctggcagt cggttggttc ggtactgacc acaaattccg ggcgaatgcc
    40441 ggattgttca ataatgtact gccagcacag accgtcgcag tcttcttcct gcacagtacc
    40501 ggtaaccagc agggtatatt catcttccag accgaggtct ttaatgattt taccggcata
    40561 aaccatagat gccatgccgc cttcctggtc ggaagtaccg cgaccaccga tcagctcatc
    40621 agtttccatg ccttcgtacg gatcgaagtc ccagtttttg atgttgccaa tgccgacggt
    40681 atcgatatga gcgtccattg ccaccagacg cgggccgtgg ccgatataac cgagaacgtt
    40741 acccatcggg tcgatttcaa ctttatcgaa gccgactttt tccatctctt ctttaatacg
    40801 atgtactacc cgtttctcgt cgcagctttc actgggaata gcaaccatgt cgcgcaggaa
    40861 gcgagtcata tccgcctggt aatcttttgc tttttcaaga atcagtttga atggaatatt
    40921 cttagccatt atttttctcc agatttttta ctccccgttt tgcagggagc agttccaata
    40981 gggtgattaa ggtgctacag cgtgtttgcc ttcccagaca acttcgcggt agtgcttcac
    41041 gtcggtgtcg ccttcagtgc tgataaccag cactacggca tctttgttca gcgccagttt
    41101 ttccatcagg ctttgacgtt gcgggtgata atgaaccgct gcgagaacgc ccaaaccgac
    41161 agcgccggat tcaccggaga tgatgcgcgg gtcgttgccg tacggattac ccagcacgcg
    41221 catacctaat gcggcaacgc tgtcctggca ggagatgaat tgggtggcgc agttacgtag
    41281 gatttcccag cccagcgggt taggttcgcc acaggccagg cctgccatga tggtggccat
    41341 atcaccgcca acgttgacga tgtcgccttt gacgccggag cgataaatac agtcagcttt
    41401 gtcaggttca acaataatgc tgtgcagatt ttgcgggcta tagacgtcga ccagataacc
    41461 cagcacacca ccggccattg ctccgacacc ggcttgcagc agaacgtgcg tcggggttac
    41521 gcccatttca cgcatttgct cgacggcttc atctgccagg gttgcgtagc cttgcatgat
    41581 ccaggttggg attttggtgt aaccttccca tgccgtgtcc tgtaccactt cccagccgtg
    41641 ctgctgcgcg tgttgcatgg tcaggcgaac ggtatcgtca tagttcatat ctgtgacgat
    41701 gcactcggca ccgaggttca gaatggcgtc aacgcgttcc tgagcagaac ctttcggcat
    41761 gtaaatcacc gcattctgtc cgagttgctg cgctgcccac gccacaccgc gcccgtggtt
    41821 gccgtcggtg gtggtcgcga aagtcatttt ttcgccgatg gcatttttca ggtgctcaaa
    41881 tgacagcgtt tcgatatcaa gatgatattt ttcacacaat aattgagcga tagcgtacgc
    41941 accgccgagc attttgaagg cgttcagacc gaatcgtttt gattcgtctt tgacaagaat
    42001 tttcttcacc ccaaaaaggt ttgcgagatc gtccagcgca caaagcggtg ttgggcgata
    42061 accagctatt ttctggtgga actggcgcgc cagtttggcc tggctttgcg aaaagagcgg
    42121 tgatgtttcg ccgttgaaaa atttgttatc ggcgatatca atcttcaatg agaaaacgga
    42181 catatcctat ccttttggcc tctggaaata gagggccgac cagctgatcg gcccgagggg
    42241 ttatttcacg cgttcttgcg cttctttcag cagttgctcg agcagtgcac ctggtttggc
    42301 gtatttacgg gacaggatca tcgcggcgat gatataaggc ttccagctgg cttctttgta
    42361 ggtagcgata cggtattttt cgaatacgcc ttcagtcact tccccttctt tgcaggatac
    42421 gccgctgata tcagctggca ggcagtgcat gtacagggct tcgccatcac gggtcagttc
    42481 catcatctct tcagtacaat gccagtcttt gtgttgcgcg ttctgtgcca gacactgttt
    42541 ttccagtgct tttaagcctt cgtgatcgtt agcgcgcagc aattcggtac gctgttccat
    42601 cactttgtaa ggtgcccatg acttcggata aacgatgtct gcatctttga acgcttcttc
    42661 catgctggtg acctgacgga agctaccgcc agatgcttta gcattgtttt tcgcgacttc
    42721 tactacatcc gggatcaggt cgtagccttc cggatgggcc agggtgacat ccataccgaa
    42781 gcgagtcatc agaccgatga tgccttgtgg tacagagagc ggtttgccat agcttggaga
    42841 gtaggcccag gtcatggcga tttttttacc tttcaggttt tccagtgaac caaagtgttc
    42901 acgtaaccat gccaggtcag ccattgactg agtcgggtgg tcaatatcgc attgcaggtt
    42961 cactaaagcc ggacgctgtg gcagtacacc ctgcttgtaa ccgtcatcaa gtgcagcgcc
    43021 aacttcacgc atataggcgt tgcctgcgcc cagatacata tcgtcgcgaa taccaatagc
    43081 gtcggcgcag aaggagatca tattggcggt ttcacgcacg gtttcgccgt gagcgatttg
    43141 tgatttgcct tcatcgagat cttgctgtgc aaggccgagc aggttaagcg cggaagcata
    43201 agagaagcgg gtacgagtgg agttgtcgcg gaatacggaa atacctaatc cactattaaa
    43261 gactttggtt gagatgtttt cagcacgcag tgcttttaat gctgcggcaa cgtccagtac
    43321 ttgtttcagt tcatctggag tctgttccca cgttaacaaa aaatctttct cgtgaaggtg
    43381 agaggtcagc gaattgatat ccttaatcag ctcattaaca gttttcatca taaagtcccg
    43441 gtagtggaat gacaggcaaa gtggtttatt tcactgtgac ttgccaaagt gattggtgtt
    43501 ttcacagcta tacctgggac taacaataag tgtgccagaa ttatccacgg ggctgcaaaa
    43561 gactaatttg acggataatg tgtggggttg atcacaaatt gatgccagct tccggttgtt
    43621 tgtaaaatac gaaaacgttt ggcttttttt atcgcgttat ttatttgctg ctaagtatca
    43681 gaaataagat aaagggacag gctatccctc gtcagtttga taaactcacc gccataattc
    43741 tcatattgat atagaaatgt gatatctctt ccataatttc attagaaagt gataaaaatc
    43801 ttatgttttt atcaaagcca atttcgcctg agttaatatg atcatgatct gtgaaccatc
    43861 aacgtcttca atgatcgact tttaagtaag tctggaaggt aaagctatgg agcttgctac
    43921 tacgcagtca gtattgatgc aaattcaacc gacaattcag cgttttgcca gaatgcttgc
    43981 cagcgttttg cagcttgagg ttgagatcgt tgatgaaaac ttgtatcgcg tcgccggaac
    44041 gggcgcgtat gggaagtttc ttggtcgcca gttgagcggc aactcacgcc tgctctgcca
    44101 cgtcctggaa acgaaaactg aaaaagttgt gacacagtct cgcttcgatc ccctttgcga
    44161 aggttgcgat agtaaagaaa attgccgcga aaaagcattt ctgggtacgc ctgtcatttt
    44221 acaggatcgt tgtgttgggg tgataagttt gattgccgtt acccacgagc aacaagagca
    44281 tatcagtgat aatttacgcg aattttctga ttatgttcgc catatatcca ccatttttgt
    44341 ttcgaaactt ctggaggatc aggggccagg agataacatc agtaaaatat tcgcgaccat
    44401 gatcgataat atggatcagg gcgtattagt tgttgatgat gaaagtcggg ttcagtttgt
    44461 taatcagact gccttaaaaa cacttggtgt tgtgcaaaat aatattattg ggaaacctat
    44521 ccgtttcaga ccattaacat ttgagagtaa ttttacccat ggacatatgc agcatattgt
    44581 ttcgtgggac gataaaagtg aattaatcat tggtcaattg cataacattc agggccgaca
    44641 attattttta atggcgtttc accaatcgca taccagtttt tctgtagcaa atgcacctga
    44701 tgaaccgcat attgaacaat tggttggcga gtgccgtgtt atgcggcaat taaaacgact
    44761 cattagccgt attgcaccca gcccatccag cgttatggtg gttggtgaaa gcggcacggg
    44821 taaagaagtt gtcgcccgtg caatccataa gttgagcgga agacggaata aaccctttat
    44881 tgctatcaac tgtgccgcga ttccggagca gcttctggag agcgaactgt tcggttatgt
    44941 taaaggcgca tttactggcg cttctgccaa cggtaaaaca gggttgattc aggcggcgaa
    45001 tacgggcacg ctgtttctcg atgaaatagg tgatatgcca ttaatgttgc aggctaaatt
    45061 actgcgcgct attgaggcac gtgaaattct gccgattggt gccagtagcc caatacaagt
    45121 cgacattcgc atcatttctg caactaacca gaatttggcc cagttcattg ccgaaggtaa
    45181 attccgcgaa gatctcttct accgacttaa tgttatcccg ataactctgc caccgctgcg
    45241 tgaacgtcag gaagatattg aactactggt gcattacttt ttacatctgc atacccgtcg
    45301 tctgggatcg gtttatcctg gcattgctcc cgatgtcgtc gaaatattgc gtaagcatcg
    45361 ttggcccgga aacctgcgcg agttaagcaa tttgatggaa tatctggtta acgttgttcc
    45421 ttcaggtgaa gttatcgaca gcacgctatt gccgccaaat ctgctgaata atggcacaac
    45481 ggagcaaagt gatgtaacag aggttactga ggcacacctg tcactcgatg atgcgggcgg
    45541 cacggcgctg gaggagatgg aaaagcaaat gatccgcgag gcgctttcac gtcataacag
    45601 caagaagcaa gttgctgatg aactgggcat cggcattgct acgctctatc gcaagattaa
    45661 gaaatatgag ttgttaaaca cataacatca gtgaatccgg cctgaattca ggccggatat
    45721 ccttttactt cgttttctcg caatccagaa ctgtatttac aatcatctga taccccgtgc
    45781 agcgacaaag atttcccgcc agtccgcgac gaatttccgt aatggttaat ggcttctcgc
    45841 gtggtttcgc cagcattgcc gtggtagcca taatcaggcc aggcgtacaa aacccgcact
    45901 gcactgcgcc ggatttcgca taagcctgct gaacatgaga aagttttccg cctttcgctt
    45961 caccttccag cgtgcggatc tcttttcctt cagcccaggc ggcaaggtat aagcaactgt
    46021 ctattgctgt gccgtcgacc agcaccgtac aggcaccaca ttcacccacg cagcaccctt
    46081 gtttgacact tagcagccct tgttcgcgga gtaattccga gagcggcgtg cctggtacgg
    46141 catgaagctg aaaaggcatc ccgttaatgg tgcattcgat ggtaattgtt tcgctgtgat
    46201 tcattgcaat tttccccccg ccgcggcgac ggcttcgcta atcacttttt tggtcatcgt
    46261 ctggatgaga tgcagacgaa actctttact ggcccgccat gaagaacgcg gggcgacatc
    46321 ttgcaggaca gattcgctga tagcttccag cgtttgcagg tttaatggcg cattttgtgc
    46381 agtctgttcg gcatgttggc agcgaatcgg cgttggcgcg gcaacaccaa atgccaggcg
    46441 taattcgctg aaattgccgt tatccagtcg gcaatgtgcg gcacagccaa tcgttgaaat
    46501 atccattgcg tcgcgcatgg catatttaaa atgcgcgctg cccacgtgtt ctttcggctg
    46561 gggcggaaaa tgaaaggcga caaggatttc gtcatgctca agagacactt tgcccggccc
    46621 ggtgtgaaag ccattaatcg ggacgaaacg aacaccgcgt ggggagtgga tctccagttt
    46681 cgcgtcataa atgagcgttg gcgtggcaga atctgcgctg gtggcaccgt tgcaaatatt
    46741 tccaccgtag gtagcgacgt tacggatctg cggtccagca atggacgagg ccgcagcaca
    46801 taacgccggg agatgacgtt gagttataga atcttctatt agctgggtaa atgtcgttgc
    46861 agagccgata cgtagcgagc catcttccgc cagcgtaatt ccccgcagct ccgccagatt
    46921 atgaatatca acaatatggc gataacggtc attgtgatgg tggagctgaa tcagtacgtc
    46981 agtgccaccg gcgagcagtt tggcctgcgg gttgtcagcc agcaggttga tggcatcggc
    47041 aagggttgct gcgcgatggt aagaagcaaa atcaaacatg atgttatcct caaatcaatc
    47101 ctgccagatg gaactcttca tataaccgtt ttggcgtcag cggcagtgta ttgattgcaa
    47161 caccggtagc catcttcacc gcgttacgaa tagcagcggc aacaggaatt attggtggct
    47221 cacccagtga cttatgtccg tatgcggatt gcggctcatt gatttcgacg aacgcgcttt
    47281 ccagttgtgg cagatccggc atggtcggca ttttgtaatc cagcagattg gggttacgga
    47341 ccacgccgct tttcgcatcg atgatcatct cttcaaatag cgcccagcca atgcccattc
    47401 ccattccgcc gtgtacctga ccttctgcca gtagcggatt aagaatatgg cccgaatcat
    47461 gaacgttgag gatgcggttg atggtgactt tgcacagcgc aatatcgacc gtcagatcaa
    47521 caaaggtaca gccgaacgcc ggtgggttag tggtggtttt gatggagctt tcagcagaga
    47581 gctgcccgcc gcgttcaggg tggtagaaag cgtccatcgc caaatctttt aacgacatta
    47641 acggctcttc cggtcgttca accagcacga tatggccttt tatcagggtc agattcatcg
    47701 ctgactgatg tagcatgact gcggcgtgag cgatgatttt ctcttttaat aacagtgccg
    47761 cactgcgcag cgcaggcgcg gcaacatagc tctggcgtga ggcaaatgcg ccgggatcga
    47821 acggcgtaac gtcggtatct tgtgttgaaa taacgcgaac gtcgctgacc ggaaccccca
    47881 cggtttctgc caccatttgc gagaagacgg tgtcggcacc ctgaccgatt tccgtcgcgc
    47941 cgctttgcac gttgatggtt ccatcctgat tcatcagaag gcgcgcgcct gctatttcta
    48001 cgccgacagg ccaggtgtta gaggtgtagc taaaacaggc gacgccaacg ccgcggcgta
    48061 aattgccttg ctggttctgg cactctgcac ggcgtttttc ccattcaaag attttccggc
    48121 ctttttcaag acactccggc aaccctgcgc tgtaaatacg tttgcccgtg agcggattag
    48181 catctccttc gcgtgaggcg ttgcgtaaac gaatttcaac aggatcaata cctaacgctg
    48241 tcgcggcgtc atcaagcata gactcaacgg caaatacgac ttgtggcgcg ccataaccac
    48301 gcatcgcacc agccgagggg aggttggtat agcaggtctt tgaactgtaa gcgtaggcac
    48361 aacgaggata aaggtaagcg actttatttc cccctgcaga agcgatggag tgcccgtgag
    48421 atacataagc gccggtgtta gacagaacat ccagactata acctttcaat gttccgtcgc
    48481 ggttcacgcc catttgcccg tcaatagtaa aagcgtggcg ggtacgggtt gcgaggaaac
    48541 actcttcacg gctaagggaa actttcaccg gaatgccgcc aagcttgctg gtcaggaatg
    48601 ccgccattgg ctcttccagt acatcctgtt tattaccaaa accgccaccg acaaatgttt
    48661 tgatgactcg tacgcatgac cagggaatat ccagcgcctg accaaccacg cggcgaacaa
    48721 tgtgcgggat ctgggtgctg gaaacgatgg taattcgcga gtcatcctcc atccacgcca
    48781 gcgatgttac gctttccatg tgacaatgtt gaataacggg ggtctgatag tgcccctgta
    48841 cctggtagtc ggcggcatcg attgtttgtt ggacattgcc cgtcgacatc gtgctttgtt
    48901 tcagtaaatt gccgccgtta tggattggtg cagcgtcctc tgccagcgcc gcttctggcg
    48961 tggtgataac gggtaattct tcccactcaa tgctgaccaa ttgcgccgct ttttctgccg
    49021 tgagttcatc gcgggccacg acgatggcaa cggcgtcacc atgatgacga acatggcgag
    49081 ttagcagtgc gcgatcggcg gtatcgcgct tgttttcgtc aagtgtccat gcatgcccag
    49141 ctgtagcgaa tgggatatca ggcacatctt cccaggtaaa aatcgccagt acgcctggta
    49201 aacttctggc ttgttcatca ttaatactta cggcataacc atgtgcgata gggctacgta
    49261 catatttcgc ataacacatg cccgccataa cataatcgtc agtatatcgt gcccgcccgg
    49321 tgaccttagc aatggcatca acgcgcatgc atgattcacc cgtagcggtt gcttcccgcg
    49381 cttccataga aatctcctcg atttgaacat ttggttctta tggcaaagat ttagtctaag
    49441 caggcaagat ttacgccaga aacggcgata agcgttaagg gaggaaggaa gtgtgatgat
    49501 gttaaaaaaa cacagatatg atacgaaaca ggaccctgaa taggggtagt gatacgtatc
    49561 aatatgagtg gcgataaaat caatgtgata aattaggatt attacgcatc aggatgggaa
    49621 atagggtaaa aaagtcagga acttcgatac ctctacagtg aatattttgc tgatgatact
    49681 tccctttgcc aaatgaaatt aacctctttc tctattacat cgtttttcca gaagcaagag
    49741 attgggtatc aagaggctgg ctgctatgat atggcgtctt gttttttaag cgaggaaaga
    49801 ttttgagtgc gggacgcctg aataaaaaat ctctgggtat cgtgatgttg ttatcggttg
    49861 gactgctttt ggcgggctgt tcgggtagca aatcatccga tacaggaacg tattccggct
    49921 ccgtttacac cgtgaaacgg ggggatacgc tatatcgtat ttcgcgcacc acgggaacca
    49981 gcgtaaaaga gctggcgcga ctgaacggca tttccccccc ttacaccatt gaagttggtc
    50041 agaaactaaa actgggtggg gcgaaaagta gcagtagtac acgtaaatca accgccaaat
    50101 caacgaccaa aaccgcatcg gttacaccgt catcagcggt accgaaatct tcctggccgc
    50161 cagtagggca acgttgttgg ttatggccaa cgacagggaa agttatcatg ccgtattcga
    50221 cagcagatgg cggcaataaa gggattgata tctcagctcc acggggtaca cctatttacg
    50281 ccgcgggtgc aggaaaggtg gtgtatgtgg gcaaccagct gcgtggctac ggtaatctca
    50341 tcatgattaa acacagtgaa gattacatta cggcttacgc ccataatgac acgatgctgg
    50401 taaataatgg gcaaagcgtg aaggctgggc aaaaaatcgc caccatgggg agcacggatg
    50461 cggcatctgt tcgcctgcat ttccagattc gttaccgtgc aacggcaatt gatccgctac
    50521 gttacttgcc gcctcagggc agcaagccaa aatgctgatg gcgaattaat cagcagtcag
    50581 cagcgcggca cttgctaaga agagcgtaag gtttataatg ccttacgcat ctcgaagcgg
    50641 gcgtagttca atggtagaac gagagcttcc caagctctat acgagggttc gattcccttc
    50701 gcccgctcca atttatcttc acctcatcaa tctgccattt ttgctgtatc tgattttcta
    50761 tattatctta attcaatcgc ttcagacttt tcgcaatctc tgtaacactc ccccattttt
    50821 ttgtaaataa aaagcgttta cgcaatgcaa tccttttgta acaacccagg taaaatacct
    50881 tattttcaag ttgttaaata agtggttatc tacgctttta acaaacgatt gatggaatat
    50941 tttatgaaag ggaaatctgc attaactctt ctgctcgctg gtatattttc gtgcggcaca
    51001 tgccaggcga caggggcaga agtgacgagt gaatctgtat ttaacattct taattccacg
    51061 ggggcagcaa cagataaaag ttatttatcg ttaaatccag ataagtatcc taattatcgt
    51121 cttctgattc actcagccaa attacaaaat gaaataaaga gccactatac caaagacgaa
    51181 attcaaggat tgctaacatt aacagaaaac acacgaaagt taacgttgac ggagaaaccc
    51241 tggggaacgt ttattcttgc ttctactttc gaagatgaca aaacagcggc agaaacccac
    51301 tatgatgctg tctggctgag ggatagttta tggggctata tggcattagt atcagaccag
    51361 ggaaatagtg tagccgcaaa aaaggttctg ctcaccctgt gggattatat gtctacgccc
    51421 gaccagatta agcggatgca ggacatcatt agcaacccaa aacgactgga tggtattccc
    51481 gggcaaatga atgctgtgca tatttgacag caactccccc gtgatggcag atgtgcagga
    51541 agaaggcaag ccgcagctgt ggaaccataa acaaaatgat gcgttaggtc tttatcttga
    51601 tctccttata caagcaatca atactggtac tattaacgct gaagattggc aaaaaggtga
    51661 ccgactaaaa tcggttgcgc tgctgattgc ttatctggat aaagccaatt tctatgttat
    51721 ggaagattcc ggagcgtggg aggaagatgc ccgattaaac acatcttccg ttgcactggt
    51781 gacctctgga ctggaacgtt tgtcaaacct actctcgaag aaagactctg tttttgtctc
    51841 tgatttacta cgcgaagcta aagtcaatga actggatgaa actctttcaa caacgaggtt
    51901 gaaccacctc atagataaag gttatgaaag aataacgtta cagcttgatt tagggggaga
    51961 atccccaggt tatctggaaa aagataaaca ttacagagaa gccgatgcgg ctttacttaa
    52021 tgttatttat cctgcaaatc tttcaaagat aaacaccaga agaaaagagc aagttctaaa
    52081 aatagttaaa aaactagcag gtccgtatgg tataaaaaga tacgagaagg ataattatca
    52141 gtcggcaaat ttttggttca atgatattaa aaccgatacc gatcaaaatt cgcatgcaaa
    52201 aagagaaaag agcttcattc catcaacaga agcagaatgg ttttttgact catggtatgc
    52261 aaaatcagca gcaattgttt ataaagaatc aagaaaagaa gagtatttga atgactcagt
    52321 acaatttatg aatagatcat tggcccaaat aacaggcgag aatatgattg gtgcaaatgg
    52381 caggagcgta ccagaaatgg cacttcccga aagctataac tatatccaca agtcgggaac
    52441 tttgcatgaa gcgccaagcc ctatcattcc gctaaattgg tcgaaagctt caatgactct
    52501 catgttgaaa gagatgtcca accttatcaa tgatgaggga attaaataat tttcataatg
    52561 aggcggtcct ggattaaaag gaccgctcaa agagaaatcg aaaggggagg taaaagtgat
    52621 caataaatta ctgttagctt acctgatagg tctggttgtg acatcgactt taatatttat
    52681 cttctcggaa gaaaaagtaa catatcggtt gtttgctgca ataattacag gtttaacctg
    52741 gccgcttagt ctcattcctt ccattataag tctcatgatt cgtaaatccg actaacaaga
    52801 aaattatatg atcgattaag aaaaatagaa tcgaacaaga gaataaaata tatgcgggga
    52861 gtaacaaaac actcgcagtg tgttaatgaa gaactaacaa atatgatttg tattgaaaat
    52921 agttaccaaa caagtgttat aagtaatacg aggtgtgttt aagtaaaaca tatcaacagt
    52981 tacaaatctg tatatggaca ggatgacgta ctagtgtaag tggctgatag ctattcgctc
    53041 ataacaaaat cattatttta taa
//
LOCUS       DAZKIF010000015        52584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZKIF010000015 DAZKIF010000000
VERSION     DAZKIF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.015"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            39..1088
                     /gene="yeiH"
                     /locus_tag="ACHOTW_000932"
     CDS             39..1088
                     /gene="yeiH"
                     /locus_tag="ACHOTW_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP9843091.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1162..2019
                     /gene="nfo"
                     /locus_tag="ACHOTW_000933"
     CDS             1162..2019
                     /gene="nfo"
                     /locus_tag="ACHOTW_000933"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9843092.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2022..3110
                     /gene="yeiI"
                     /locus_tag="ACHOTW_000934"
     CDS             2022..3110
                     /gene="yeiI"
                     /locus_tag="ACHOTW_000934"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9843093.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3166..4416)
                     /locus_tag="ACHOTW_000935"
     CDS             complement(3166..4416)
                     /locus_tag="ACHOTW_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9843094.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4516..5457)
                     /gene="rihB"
                     /locus_tag="ACHOTW_000936"
     CDS             complement(4516..5457)
                     /gene="rihB"
                     /locus_tag="ACHOTW_000936"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP9843095.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            5587..6285
                     /gene="yeiL"
                     /locus_tag="ACHOTW_000937"
     CDS             5587..6285
                     /gene="yeiL"
                     /locus_tag="ACHOTW_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP9843096.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6356..7606)
                     /gene="psuT"
                     /locus_tag="ACHOTW_000938"
     CDS             complement(6356..7606)
                     /gene="psuT"
                     /locus_tag="ACHOTW_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9843097.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7700..8638)
                     /gene="psuG"
                     /locus_tag="ACHOTW_000939"
     CDS             complement(7700..8638)
                     /gene="psuG"
                     /locus_tag="ACHOTW_000939"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP9843098.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(8626..9567)
                     /gene="psuK"
                     /locus_tag="ACHOTW_000940"
     CDS             complement(8626..9567)
                     /gene="psuK"
                     /locus_tag="ACHOTW_000940"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP9843099.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(9991..11682)
                     /gene="fruA"
                     /locus_tag="ACHOTW_000941"
     CDS             complement(9991..11682)
                     /gene="fruA"
                     /locus_tag="ACHOTW_000941"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP9843100.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(11699..12637)
                     /gene="fruK"
                     /locus_tag="ACHOTW_000942"
     CDS             complement(11699..12637)
                     /gene="fruK"
                     /locus_tag="ACHOTW_000942"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9843101.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12637..13767)
                     /gene="fruB"
                     /locus_tag="ACHOTW_000943"
     CDS             complement(12637..13767)
                     /gene="fruB"
                     /locus_tag="ACHOTW_000943"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP9843102.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14135..15316
                     /gene="setB"
                     /locus_tag="ACHOTW_000944"
     CDS             14135..15316
                     /gene="setB"
                     /locus_tag="ACHOTW_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP9843103.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15313..15567)
                     /gene="yeiW"
                     /locus_tag="ACHOTW_000945"
     CDS             complement(15313..15567)
                     /gene="yeiW"
                     /locus_tag="ACHOTW_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9843104.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15722..16294
                     /gene="yeiP"
                     /locus_tag="ACHOTW_000946"
     CDS             15722..16294
                     /gene="yeiP"
                     /locus_tag="ACHOTW_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP9843105.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16517..17983
                     /gene="yeiQ"
                     /locus_tag="ACHOTW_000947"
     CDS             16517..17983
                     /gene="yeiQ"
                     /locus_tag="ACHOTW_000947"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9843106.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            18101..19087
                     /gene="yeiR"
                     /locus_tag="ACHOTW_000948"
     CDS             18101..19087
                     /gene="yeiR"
                     /locus_tag="ACHOTW_000948"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP9843107.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19126..19839
                     /gene="lpxT"
                     /locus_tag="ACHOTW_000949"
     CDS             19126..19839
                     /gene="lpxT"
                     /locus_tag="ACHOTW_000949"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP9843108.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20252..20818
                     /gene="mepS"
                     /locus_tag="ACHOTW_000950"
     CDS             20252..20818
                     /gene="mepS"
                     /locus_tag="ACHOTW_000950"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP9843109.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            20999..22555
                     /gene="pdeN"
                     /locus_tag="ACHOTW_000951"
     CDS             20999..22555
                     /gene="pdeN"
                     /locus_tag="ACHOTW_000951"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9843110.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22637..24451
                     /gene="yejA"
                     /locus_tag="ACHOTW_000952"
     CDS             22637..24451
                     /gene="yejA"
                     /locus_tag="ACHOTW_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9843111.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            24452..25546
                     /gene="yejB"
                     /locus_tag="ACHOTW_000953"
     CDS             24452..25546
                     /gene="yejB"
                     /locus_tag="ACHOTW_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP9843112.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25546..26571
                     /gene="yejE"
                     /locus_tag="ACHOTW_000954"
     CDS             25546..26571
                     /gene="yejE"
                     /locus_tag="ACHOTW_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9843113.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26573..28162
                     /gene="yejF"
                     /locus_tag="ACHOTW_000955"
     CDS             26573..28162
                     /gene="yejF"
                     /locus_tag="ACHOTW_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP9843114.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(28166..28510)
                     /gene="yejG"
                     /locus_tag="ACHOTW_000956"
     CDS             complement(28166..28510)
                     /gene="yejG"
                     /locus_tag="ACHOTW_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP9843115.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28843..30033)
                     /gene="bcr"
                     /locus_tag="ACHOTW_000957"
     CDS             complement(28843..30033)
                     /gene="bcr"
                     /locus_tag="ACHOTW_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP9843116.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(30061..30756)
                     /gene="rsuA"
                     /locus_tag="ACHOTW_000958"
     CDS             complement(30061..30756)
                     /gene="rsuA"
                     /locus_tag="ACHOTW_000958"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP9843117.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30905..32665
                     /gene="radD"
                     /locus_tag="ACHOTW_000959"
     CDS             30905..32665
                     /gene="radD"
                     /locus_tag="ACHOTW_000959"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9843118.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32790..33074
                     /gene="rplY"
                     /locus_tag="ACHOTW_000960"
     CDS             32790..33074
                     /gene="rplY"
                     /locus_tag="ACHOTW_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP9843119.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33213..34220)
                     /gene="yejK"
                     /locus_tag="ACHOTW_000961"
     CDS             complement(33213..34220)
                     /gene="yejK"
                     /locus_tag="ACHOTW_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP9843120.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34402..34629
                     /gene="yejL"
                     /locus_tag="ACHOTW_000962"
     CDS             34402..34629
                     /gene="yejL"
                     /locus_tag="ACHOTW_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP9843121.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34649..36409
                     /gene="yejM"
                     /locus_tag="ACHOTW_000963"
     CDS             34649..36409
                     /gene="yejM"
                     /locus_tag="ACHOTW_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP9843122.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36484..36560
                     /locus_tag="ACHOTW_000964"
     tRNA            36484..36560
                     /locus_tag="ACHOTW_000964"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36518..36520,aa:Pro,seq:ggg)
     gene            complement(36661..39252)
                     /gene="yejO"
                     /locus_tag="ACHOTW_000965"
     CDS             complement(36661..39252)
                     /gene="yejO"
                     /locus_tag="ACHOTW_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFP9843123.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            39572..40219
                     /gene="narP"
                     /locus_tag="ACHOTW_000966"
     CDS             39572..40219
                     /gene="narP"
                     /locus_tag="ACHOTW_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP9843124.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(40254..41306)
                     /gene="ccmH"
                     /locus_tag="ACHOTW_000967"
     CDS             complement(40254..41306)
                     /gene="ccmH"
                     /locus_tag="ACHOTW_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP9843125.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(41303..41860)
                     /gene="dsbE"
                     /locus_tag="ACHOTW_000968"
     CDS             complement(41303..41860)
                     /gene="dsbE"
                     /locus_tag="ACHOTW_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP9843126.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(41857..43800)
                     /gene="ccmF"
                     /locus_tag="ACHOTW_000969"
     CDS             complement(41857..43800)
                     /gene="ccmF"
                     /locus_tag="ACHOTW_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP9843127.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(43797..44276)
                     /gene="ccmE"
                     /locus_tag="ACHOTW_000970"
     CDS             complement(43797..44276)
                     /gene="ccmE"
                     /locus_tag="ACHOTW_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP9843128.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(44273..44482)
                     /gene="ccmD"
                     /locus_tag="ACHOTW_000971"
     CDS             complement(44273..44482)
                     /gene="ccmD"
                     /locus_tag="ACHOTW_000971"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP9843129.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(44479..45216)
                     /gene="ccmC"
                     /locus_tag="ACHOTW_000972"
     CDS             complement(44479..45216)
                     /gene="ccmC"
                     /locus_tag="ACHOTW_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP9843130.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(45258..45920)
                     /gene="ccmB"
                     /locus_tag="ACHOTW_000973"
     CDS             complement(45258..45920)
                     /gene="ccmB"
                     /locus_tag="ACHOTW_000973"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP9843131.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(45917..46540)
                     /gene="ccmA"
                     /locus_tag="ACHOTW_000974"
     CDS             complement(45917..46540)
                     /gene="ccmA"
                     /locus_tag="ACHOTW_000974"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP9843132.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(46553..47155)
                     /gene="napC"
                     /locus_tag="ACHOTW_000975"
     CDS             complement(46553..47155)
                     /gene="napC"
                     /locus_tag="ACHOTW_000975"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP9843133.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(47165..47614)
                     /gene="napB"
                     /locus_tag="ACHOTW_000976"
     CDS             complement(47165..47614)
                     /gene="napB"
                     /locus_tag="ACHOTW_000976"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP9843134.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(47611..48474)
                     /gene="napH"
                     /locus_tag="ACHOTW_000977"
     CDS             complement(47611..48474)
                     /gene="napH"
                     /locus_tag="ACHOTW_000977"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP9843135.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(48461..49156)
                     /gene="napG"
                     /locus_tag="ACHOTW_000978"
     CDS             complement(48461..49156)
                     /gene="napG"
                     /locus_tag="ACHOTW_000978"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP9843136.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(49163..51649)
                     /gene="napA"
                     /locus_tag="ACHOTW_000979"
     CDS             complement(49163..51649)
                     /gene="napA"
                     /locus_tag="ACHOTW_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFP9843137.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(51646..51909)
                     /gene="napD"
                     /locus_tag="ACHOTW_000980"
     CDS             complement(51646..51909)
                     /gene="napD"
                     /locus_tag="ACHOTW_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP9843138.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(51899..52393)
                     /gene="napF"
                     /locus_tag="ACHOTW_000981"
     CDS             complement(51899..52393)
                     /gene="napF"
                     /locus_tag="ACHOTW_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP9843139.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            52502..>52584
                     /gene="yojO"
                     /locus_tag="ACHOTW_000982"
     CDS             52502..>52584
                     /gene="yojO"
                     /locus_tag="ACHOTW_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFP9843140.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAH"
BASE COUNT        12793 a        14246 c        12949 g        12596 t
ORIGIN      
        1 gttagtcgta accttatgcc cggttaaagg agagggttat gacgaatatc accttacaga
       61 aacaacatcg tacactgtgg cattttattc cggggttagc cctgagtgca gttatcaccg
      121 gggtcgccct gtggggtggt tccattcccg cggttgcggg tgccgggttt agtgccctca
      181 ccctcgcaat cttgttgggg atggttttag gcaacaccat ctatccgcac atctggaaaa
      241 gctgtgacgg tggtgtgctg tttgccaagc aatatttatt acgtctgggt atcattcttt
      301 atggcttccg tctgacgttc tcgcaaattg ccgatgtcgg tatcagtggg atcatcattg
      361 acgtgctgac gctgtccagt accttcctgc tggcttgctt cctggggcag aaagtgtttg
      421 gtctggataa gcacaccagc tggttgatcg gtgccggtag cagtatctgt ggtgctgccg
      481 cggtactggc gactgagccg gtagtgaaag cggaagccag taaagtaacc gtggctgttg
      541 caaccgttgt tatcttcggg accgtcgcga ttttcctcta cccggcgata tatccgctga
      601 tgtctcaatg gtttagtccg gaaactttcg gtatctacat tggttctact gtgcacgaag
      661 tggcgcaggt ggtggcggca ggtcatgcca tcagcccgga tgcggaaaac gcagcagtta
      721 tttccaaaat gctgcgcgtg atgatgctgg ctccgttcct catcctgctg gcggcgcgtg
      781 ttaaacagct gtctggggcg aacagcggcg agaaaagcaa aatcactatt ccgtggtttg
      841 ccatcttgtt catcgtagtt gccatcttta actcgttcca cctgttaccg cagagcgtgg
      901 tgaacatgct ggtaacgctg gataccttcc tgctggcaat ggcgatggcg gcgctgggtc
      961 tgaccactca cgtcagcgcg ctgaaaaaag ctggggcgaa acctctgctg atggcactgg
     1021 tactgtttgc ctggctgatt gttggtggtg gtgcgattaa ctatgtgatt caaagcgtca
     1081 tcgcataaac cactacatct tgctcctgtt aacccgctat cattaccgtt ttcctccagc
     1141 gggtttaaca ggagtcctcg catgaaatac attggagcgc acgttagtgc tgctggcggt
     1201 ctggcaaatg ccgcaattcg cgccgccgaa atcgacgcaa ccgcgtttgc cttgttcacc
     1261 aaaaaccaac gtcagtggcg tgccgcaccg ctcacgacgc aaaccatcga tgaattcaaa
     1321 gccgcctgtg aaaaatatca ctacacatcg gcgcaaattc ttccccacga cagttatctg
     1381 attaacctcg gacatccggt cactgaagct ctggaaaaat cgcgcgatgc ctttatagat
     1441 gaaatgcagc gttgcgaaca gctggggctt tctttgctca acttccaccc tggcagccat
     1501 ctgatgcaga tttcagaaga ggattgcctt gcgcgtattg ccgaatccat caacattgcg
     1561 ctggataaaa ctcaaggtgt gacagcggtg atagaaaaca ccgccggtca gggcagtaac
     1621 ttagggttta aattcgaaca tctcgcggcg attatcgacg gcgtggaaga taaatcccgc
     1681 gtcggcgtct gcattgatac ctgccatgct ttcgctgccg ggtatgattt gcgtactcca
     1741 gccgaatgcg agaaaacatt cgcggatttt gcccgtactg tcggctttaa gtatctgcgc
     1801 gggatgcacc ttaacgatgc gaaaagcacc tttggcagcc gcgttgaccg ccatcatagc
     1861 ctcggtgaag gcaatatcgg tcatgatgcg ttccgctgga tcatgcagga cgaccgtttc
     1921 gacggcattc cgctgatcct cgaaaccatc aacccggata tctgggcaga agagatcgcc
     1981 tggctgaaag cgcaacaaac tgaaaaagcg gtagcctgaa gatgaataac cgggaaaagg
     2041 agatccttgc aattttacgg cgtaacccgc tgattcagca gaacgaaatt gcggacatgc
     2101 tgcaaatcag ccgttcgcgc gttgcggcgc atattatgga tttaatgcgc aaaggccgga
     2161 ttaaaggcaa aggttacatt ctcaccgagc aggaatactg cgtagtggtg gggacaatca
     2221 atatggatat tcgcgggatg gcggatatcc gttacccgca atcggcttct catcccggta
     2281 caattcattg ctcagcgggc ggcgtgggac gcaacatcgc ccacaatctg gcgctgttag
     2341 gccgtgacgt ccatttgctt tcagtgattg gcgatgactt ttatggcgaa atgctcctgg
     2401 aagaaacgcg ccgcgccggc gtgaatgtct ccggctgcgt tcgtttacat ggtcaaagca
     2461 catcgacgta tctggcaatt gccaatcgag acgatgaaac cgtgctggcg attaacgata
     2521 cccatctgct ggaacagtta tcgccgcaat tattgaacgg gtcgcgcgat ttacttcgtc
     2581 atgcgggcgt ggtactggct gattgcaacc tgacagccga ggcgctggaa tgggtcttta
     2641 ccctcgctgg tgagatcccg gtgtttgtcg ataccgtttc agaattcaaa gcgggcaaaa
     2701 tcaaacactg gctggcgcat attcacaccc tgaaacccac tttaccggag ctggaaattt
     2761 tatggggaca ggcgatcacc agcgatgctg accgtaatgc tgcagtgaat gcgttgcatc
     2821 agcaaggtgt tcagcaactg tttgtttatt tgcccgatga gtcagtttat tgcagcgaaa
     2881 aggatggaga acaatttttg ctgaccgcac cagcgcatac gacagtagac agttttggtg
     2941 ctgacgatgg ttttatggcg ggcctggtat atagctttct ggaaggaaac aatttccgtg
     3001 acagcgcccg ttttgcgatg gcctgcgcgg caatttcacg cgccagcggc agcttaaaca
     3061 accctaccct gtctgccgat aacgcgcttt cattagtgcc aatggtgtaa caatgttgcc
     3121 ggattcgacg cttgaccgca tcttatccgg ctcataaatc gcacactacg ctaaaccaat
     3181 aaagaacccg gcaatggtgg cactcatcag gttagaaagt gtcgccgccg ccagcgcgcg
     3241 taaaccaagc tgggcgattt ccggcgcacg gtgtggcgca accgcagaaa acgcccccac
     3301 caccaccccg atagaaccaa agttagcgaa accgcacaac gcgaaggaaa taatcgccac
     3361 ggttttagca tccagagtgc cagccgtttg cagatagggt gagaaattga gataagcgac
     3421 aaattcattt attgccagtt tctgtccaat caaactcccg gcaagattcg catcactcca
     3481 gtcaacaccc attacccacg ccagtggggc cagcagataa cctacaatgg actccagcga
     3541 ggcatgttca aaaccaaacc agccgccaac gccgccgata ataccgttaa ttaacgcaat
     3601 gatggcgacg aatgccatca ctactgtcgc cacacctgcg gcgattttca gcccggtcat
     3661 tgcccctgtc gcggcggctt caataatgct ttttggcggt gtttcggtga aagagaggtt
     3721 attaaaagaa acctgcgaag attccgtagc cgggcttagc aggcgggcaa acaagatccc
     3781 cccggggatc gccattaacg atgccgccag caaatattcc acaggcacgc ccagtgcggc
     3841 ataaccaatc attgtcgaac cagcaatcga ggccatgccg ctacaaatcg ctgtaaataa
     3901 ttcattgcga ttcagccgat cgataaaggg cttcacaatc gccggaattt cgttttgccc
     3961 gaggaaaatg gtagtgaccg cgacgaatga ctcaatcttg ctgatattta atgctttctg
     4021 gaatataccg ccgagaatgc gaattaaaat ccccatcaca ccgatgtagt agagaatact
     4081 caccagtgca gtgacgaaga taattgccgg taataccctg aaaccaaaga taaatcctgc
     4141 gccatcaaat aaggtgtcca ttttcggtcc gaccagagaa ccgaagataa atgcgctacc
     4201 cgcgtcgctg tacgccatca ctttatgcac accaaaagcg actttttcag cgacccaacg
     4261 ccctggcggt aaccaaagca taatgccgcc aatcacgacc tgtaacacta acgccgcgcc
     4321 aacggtacgc aggctgatct tcttcttgtt tactgacagt aaaaacgcaa tcgtcagcaa
     4381 taccaccatt cccagaacac ttctcatgac atccatagtg attatctctt catgtcatat
     4441 tgggcgacgg ctttctgccc atcagaaata gccaggcctg catcttgcga taccggcccg
     4501 gcttgttatc aacacttaat gggttttgat gtagccgcgc acgcactctt cgactaagcc
     4561 ccagaaccag tcgctatcaa tagtgatgcc gactttggta ttagcgggct tgccaagaac
     4621 gcccagctcg tcgcagacgg tacgcccata acaagggcca ctgttgacgt ccacttcgac
     4681 gtacatctcc tgggttttaa tgccatcagg gttaatcaga taaccgatgc aggtggcgtc
     4741 gtgcaccggg ccgccagcca ggccgtagtt ttcgaactgg gttttgagag tgaagttcat
     4801 gatgtcgctg aacagctctc cggcagggcc gcctgccctt tccattcgtg caatcacgtc
     4861 cggggtgcaa acggtctggt tggtgagatc gaggcccatc atcactaatg gaacgccaga
     4921 ggtgaacacc acgcgtgcgg cttccgggtc agcaaagatg ttgaactcgg cagatggcgt
     4981 gaagttgcct gtaccgtaag cgccgcccat tagcacaatt tcacggattt tgggcaggat
     5041 cgcgggttgc atacgcattg ccaccgcgat atttgaaagc ggaccaaccg gcaccagagt
     5101 gatatcgcca tcgcttgcca tcagggtatc aatgatgtat ttcaccgcat gagtgctttc
     5161 tgcctggcgg gtcagcggct cgaataccgg gccatccagt ccggtatcac cgtgaatatt
     5221 atcggcaacg atttgttgcc gcataatggg ttgtggcatc cccgcataaa ccggaacatt
     5281 aatctccagt ttctggcaaa cattcaggcc attaattaat gttttatcaa gcgtctgatt
     5341 acccgctaca atagtgatgc ctaataaatc tattgccgga tgtttcgccg ccatcattat
     5401 agcaatagca tcatcatgac ccggatcaca atccagaata atttttctct tttccattgt
     5461 ttatttcctc tgtttccagt tgcgttattt tttctacagc aaagaaattt cgatcgggcc
     5521 gatgattgaa tcttaacaac agcgtacgta tgctaaatat gagaaatctc atagcggata
     5581 aacatcgtga aagaaatcca caataatgat cttaagcagc aattgatgag tgaatccgcg
     5641 tttaaggatt gctttttaac ggatgtttca gccgatacgc ggctgtttca ttttttagcg
     5701 cgtgactaca ttgtgcagga agggcaacag ccgtcctggc tgttttacct gacgcgaggc
     5761 cgcgccaggc tttacgccac gctagctaat ggtcgcgtgt cgctgatcga tttctttgcc
     5821 gccccctgtt ttattggtga gattgagtta atcgataaag accatgaacc gcgtgcggta
     5881 caggctattg aagagtgttg gtgccttgcg ctccctatga aacattaccg tccgctgtta
     5941 ttaaacgaca ccctattttt acgaaaactt tgcgtcacct taagtcataa aaattatcgt
     6001 aatattgttt ctttaactca gaatcaatca tttccgttag tgaatcgcct ggcagcattt
     6061 atattactct cacaagaagg tgatctttat cacgaaaagc atacgcaagc ggcagagtat
     6121 ttaggcgttt cttatcgaca tcttttatat gttctcgcgc aattcattca cgacggttta
     6181 ttaattaaaa gcaagaaagg atatctaatt aaaaacagaa agcagttgtc agggctggca
     6241 ctggagatgg acccggagaa taaattctcc gggatgatgc agtaaaaatt atttgcaata
     6301 gcgcgattgc cggatgcaac gcttgatgcg ttttatccgg tctacaaatt gagcattacg
     6361 ccagaccaat aaagaacccg gcaatggtcg cgctcatcag gttggaaagc gttgctgctg
     6421 ccagcgcccg taaaccaagc tgagcgattt ccggtgcgcg ttttggcgaa atagccgaaa
     6481 atgccccaac gacaacaccg atagaaccaa agttagcaaa accacaaagc gcaaaggaga
     6541 taatcgcaat ggttttcact tccagcgtgc cgcccgtttg caggtatggg gagaaactca
     6601 ggtaagcgac gaattcgtta atcgccagtt tctgcccaat caggctaccc gcaaggttgg
     6661 catcactcca gtccacaccc atgatccacg ccagcggtgc cagcacatag ccaaaaatac
     6721 tttccagaga agcattggcg aagccaaacc agccgccaat tccgccgata atgccgttga
     6781 tcagcgcaat aattgccaca aatgccatta ccaccgtcgc cacaccagcg gcgattttca
     6841 gcccggtcat cgcaccgctc gccgctgctt cgataaagct ttttggcggc gtttcgctga
     6901 acgacagatt ttcaaacgtg acctgcgaaa gctcggttgc tggactaaga atacgtgcaa
     6961 acaaaatacc gccggggatc gccatcagcg atgccgccaa caggtagtca attggtacgc
     7021 ccattccggc ataaccaatc atcatcgaac cagcaatgga cgccatcccg ctacaaatag
     7081 ccgtaaacaa ctcgtttcga ttcatgcgat cgataaacgg tttaacgatc gccgggatct
     7141 cattttgccc gaggaaaata gtggtgactg cgacaaaaga ttcgattttg ctgatgttga
     7201 gggctttctg gaaaatgctg ccaaggatgc gaatcagcag ccccatcacg ccaatgtagt
     7261 acagcagact aatgagcgca gtgacgaaga taatcgccgg aagtacgcga aaggcgaaga
     7321 taaaacccgc accgtcaaag aggacatcca ttttcggccc gaccagcgaa ccaaaaatga
     7381 aggcgctacc agcatcactg taagacatca ctttatgaac gcctaatgcc gcctgttcta
     7441 cagcccactt tcccggcgga aaatagagca tgatgccacc aagagcaatt tgcagcagta
     7501 gtgcggctgc aaccgtacgc aaactgatgc tctttttatt cactgacaac agaaatgcta
     7561 ttgccagtaa caccaccatc cccacaacac ttctcattat gtccataatg attttccctt
     7621 catgccagta aacccggcgt cagcgccagg tttcggtatg tttgatgagt acgggcgacg
     7681 gctttctgcc cgtcagaaat taacccgcga gacgctgata ttctttggca atttcgctcg
     7741 ccagaatggc gttgttgaac acaagctgga tgttggattt caggctgtca ccgccggtca
     7801 gctcagcaac gcgcgccagc aggaatggcg tactttcttt accaataaca ccctgcgctt
     7861 cagcttcagc taccgcctga tcgatcaccg cattgatagt gtgttctggc atcgcaaact
     7921 gttccgggat cgggttcgct accacgaggc caccgttcag cccgctttgc catttcaccg
     7981 ccattgcacg ggcaatttca ctggcgctgt cgagacgaat gctgacgtca aacgggctgg
     8041 tacggcagaa aaacgcaggc agcgctttag tctgatagcc aattaacggc acaccgaagg
     8101 tttctaaata ctcagtggtt aatcctaaat cgagaataga tttcgccccg gcacaaacaa
     8161 cggtgacatt agtatttgcc agttcttgca aatcggcaga aatatcgaag gtatgttccg
     8221 cccctcgatg aacaccacca attccgccgg tggcaaatac tttaattccg gcaagcgccg
     8281 caataatcat cgttgaagcc acggtggttg caccattttt tcctgcggca acgacaaaag
     8341 gtaaatcgcg acgactaact ttagtcacgt tatgcccttc acgacccagt aattcaattt
     8401 cttctttgct taaacccact ttcatcacgc cgccaataat ggcgatagtg gcaggtactg
     8461 cgccctgttt acgaatagtt tcttctactt caattgcggt ctgggcattt tgtgggaacg
     8521 gcatcccgtg agaaataatg gtcgattcca gcgccacaac cggttttttg ttttttaaag
     8581 cgtcctgcac ttccggggaa atttgtaata attcagggga aatttttaat tcagacattc
     8641 tgcgttctcc actaacgata taacgttggc aatcgataaa tcggggttat tggtgtattc
     8701 acaggagagc gccattgacg aacatccctg tgcgaaacga acagattcgg caaacggcat
     8761 tccgtctacc caacacgaag caagtcccgc catcatggca tcgcccgctc cggtaacatt
     8821 aataacattg gttttgatcg gcgcagacca gccactttca ccgctgatat cgctgtaata
     8881 aacgccgtcg ccgcccatgc tcaataccag tcggttcagg ccatgttgat ggaaccaggc
     8941 agcaactttt gccacatctt cacgccctga cagcgcaatc ccactcaggg tttccgcttc
     9001 aaggcggttt ggcttgagag tatggatttg acttaatcgc tcgcgtactt tgacgcattt
     9061 ccacgcggaa accggatcga caaatacggg tacgttggca gcattatcca gaatccatgc
     9121 cagtgcctct tcactgatat tacagtccgc gacaatgacc tttgccctct gaataaattc
     9181 acggtgctgt gcgagatatt cagctgtaat agcgttgcta atattcatgt catttatagc
     9241 aaccagcatt tcaccggtat tatcgagtaa tgataaatag ctcgacgtat tttctcccgg
     9301 cacaatcagg catttatcga cataaacgcc agattgattg gtttgcgtta gcagcgattg
     9361 gccataaaaa tcactgccta cggcgctcag tagccaggct ttattaccca gcaacgccag
     9421 gttttgtgca atattgcgcc ctaccccacc aggcgtaaat tttattttac ctggatttga
     9481 atccgcataa tttaatgatt catgtgaata tccggcgaca tcaatattcg ccgagcctat
     9541 aattacgaca taatcctttt cgcgcatagc acgtcctctg gtaaaaaatt accaaccctg
     9601 tcaatccaca aggatataga tactgcaaag acggtttaaa catgtgttca tatttaaaca
     9661 tgtgctcata gtatgcatgt tttacgtaaa gtaaattgcc gccagagagg caaagcgggc
     9721 agatttgagc aggctcacaa aatgcgatta aatgcctgaa ttacaaaagc gaggaatttg
     9781 tgacgggatg cacagaacat gaacactaaa aaagggcaga aaagaatctg cccttgagga
     9841 aataacctta gtgtttgatg aacgtttcag gtttctactg aaagataaaa ccatttaaat
     9901 tcataaaatt acattcacaa accggtcatt ctctttgcgc tcatccaatg atacggggca
     9961 gtaaatttct gccctgtaac acacctttta ttacgcggct ttcgctactg cgtccacttc
    10021 cggacgtttc aggaaggcat aggccaaacc cgccaccagc gtaccggcaa taattgctac
    10081 caggtaaccc agtaccggcg taatagcgcc agggatcagc agaacaaaca gaccaccgtg
    10141 cggtgccatc agtttcgcac caatcgccat tgagattgcg ccagtcagcg ccccaccaac
    10201 gatacaacac ggcagcacac gcatcggatc acgagcagca aaaggaattg caccttccga
    10261 aataaagcac agaccaagaa ccagagcggc tttgccacct tcctgctgcg ctttgtcaaa
    10321 tttgcgacgc gccaccattg ttgccagacc cattgccagc ggtggcacca tacctgccgc
    10381 cataatcgcc gccatcgggc cataggtttg agtactcagc agacccacac cgaatgcgta
    10441 cgctgcttta tttaccggac cgcccatgtc agtacacatc atgccaccga ggatcgcccc
    10501 cagcagaacc gcattcgcag tccccatggt ctgcagccag tgagtcaacc cttcgagaat
    10561 gccagcaacc ggtttaccga tcaggtagat catcgccaga ccgaccacca gactggaaat
    10621 tagcggaatg atcaggatcg gtttcagcgc ctccatactc tgtggcagtt tcagttgcgt
    10681 actgattaac ttcgcaatgt aaccagccag gaagcccgca ataataccgc caatgaagcc
    10741 agaaccggtg ctgaccgcca gcataccgcc aatcagaccc ggcgtgagac ccggacgatc
    10801 ggcaatggag aaggcaatat aacctgccag taccggcacc atcagcgcaa aggctgaacc
    10861 accaccaatc tgcatcagcg ccgctgccaa cgtacccggc tctttaaacg cttcgatacc
    10921 aaaagcaaaa gaaagcgcga tacacagacc acctgcaacg accatcggca gcatgtaaga
    10981 gacgcccgtc agcaagtgac ggtatgcgcc tgcactctcg ttcttacctt cagaggtcgc
    11041 cgtttgagct ttgcccgccg gttcatacgg cgttgcttca gcaaccgctt tatccagttc
    11101 ctgcgcggtt ttcttcagcg ccagaccggt agaggtgcga tacatcggtt taccagcaaa
    11161 tttcgccaga tccacttcga tatctgccgc cacaatcacc agatccgctg cggctacttc
    11221 ttcgggagtg attgcattac ccgcgccaac agaaccacgg gtttcaactt tcacccacca
    11281 gccacgtttt ttcgcttcgg tttcaatggc ttcagccgcc ataaaggtgt gtgctacgcc
    11341 agtcgggcaa gcagtcaccg caactacgcg tttcggaccg ctggcggcaa ccggtgctgt
    11401 cgcagcgacc ggcgcagtgt aaggtttcgc atggcctttg gcttcactca ggaacagctc
    11461 agggtgcgca actgcccggg aaatatcgcc cagccagaca tttttaccgt tcagcgcgct
    11521 gtcattcggg atggaatcac cgagaacaat cgccatttct gcgtcgttcg gattgtcgat
    11581 gatttccagt tttgcttttc gcgccgccgc gcccagcagg gtcttcgcca tataggcgcg
    11641 tgcctgaccg agattagcgt caataatcag cagcgttttc attatgcctc tcctgctgtc
    11701 agttaaaagg ttgtaagtcg acgcgcgcca tcattgcggc caactgcgga cgatcggtaa
    11761 tacccacatt gctttgactt accgccaggg ctgcaacagc agtcgccaga cgcagtgtgt
    11821 gttcactgga ttcacgcatt agcaagccat aaatcaggcc accaaccata gaatcccctg
    11881 cgccaacggt gcttacgaca tcgactgacg gtggtttggc gatccattcg ccggaggcat
    11941 taacccaaag cgcgccttcg gcacccagtg aaataacgac atgcgcaata ccttgttcac
    12001 gcagcgcatg cgcagcttca atcacatctt tcatttcagg cagtttacgg cctgcccaga
    12061 tttccagctc gcggcggtta ggtttcacca gccacggtgc cgctttcaaa cctgctacta
    12121 acgcttcacg gctactatca aagataatgc aagggcactg actacgcagg cgagtcatcc
    12181 agtcggtgaa cgcttccggg ctgacgcctg acggtaagct tccgctgaca cagaccatat
    12241 cgaactgacc gagccagctc agagaatcag tcacaaagcg ttcccagtcg gcgggggtga
    12301 cttcaaaacc cgagaagttg aagtcggtca cttcgccgtc tttttccgtc agcttaacgt
    12361 taattcgggt gcgcccctgt acaacctgga aacggttggc aatgcccagc tcgctgaaca
    12421 gttgctgaaa accatcctga ttgtctttac cgaggaagcc gccaacggtg acatcaattc
    12481 ccaggtcttt taatactttg gccacgttga tgcctttacc cgccgcatgc agaccggtgg
    12541 ttttcaccag gttcacttcg ccgcgttcaa tttccgggca gaaaccaaca aggtcataag
    12601 ccggattaag ggtgatagta gcaacacgtc tgctcattat gcgccctccc caagaccagc
    12661 agcgatagcg tcgccgattg ctttcagcgc ctgttcagca tctgcaccct gggcggtaaa
    12721 gcgtaggcga tgacctttct taacgccaag tgccacaact ttcatcagac tacgtccgtt
    12781 tgccggtttg ccggtgccat caaggtttgt cacggtaata tcactgttaa attgtttaat
    12841 ggtgttgacc agcatggtac ctggacgagc atgcaggccg tgttcattgc gcaccacaaa
    12901 ctccgcgctt aacacgtcgt cggtcggcgc atcatcgctg gtcagcagcg ccagcaacgt
    12961 tgccgcatcc gctttcagca agcggtcagc tttattgtcg agcaacaaat cagcgagacg
    13021 cttaagaacc gcaatgggct gatcgtcatt catcgccaca ctcaccagca tggctgccgt
    13081 ttcgccgtcc acatcaaaag catttgccgc acggcttacc gcaattgcgc tacgcagatt
    13141 gccttcggcg ctatcgctca gccagatacc ctgtccgagg ttcagcggtt gttcattgat
    13201 ggctttggtg acgaaagtgg cgtcaactgc ccccgcctct ttcagacgcg cagcgttcag
    13261 cgcctgaaga gtcagcagat cgctggcgac gatatccagc gtcagcattt cgttgtcgag
    13321 cttcagctgc tcactctgct tttcgcccat cagcaatgcg cgaagttctt ctgctgttgt
    13381 tgctgacttc agttgttcag caacggaatc atcgctcagt acgtgggtca gctggcgtag
    13441 caggcccaga tgctcatccg agctggcagc aataccgatc gctacgtacg ctacctgacc
    13501 gtcaccccag gtgacgcctt ccgggaactg aaatacctga acgccggttt tcagcacctg
    13561 atcgcgggtg tcggtagtgc cgtgtggaat agcaatacca ttgccgagga acgttgaggt
    13621 ttgctgttcg cgcgccagca tgccattgac gtagccttct gctacattac cggcctgcac
    13681 cagcgccgca gcgacctggc gaatcgcctc ttctttgtct ccggcctttt cgcccggatg
    13741 gatgtcctgt acggataact ggaacatagt tctcctctct tgctgaattg aaacgattca
    13801 gcttctatga gaaaaaaagc gccaacctgg cttagggtta aagacaagat cgcgctgaaa
    13861 cgtttcaaga aagcataata cttctgtttc agcacgcaag gaaagccgta aagttatgag
    13921 caaaagtttg atgtgctgca caattttttg tcatttttcc taattgctga tgggaaaatc
    13981 cgttatcagc aatttcattt cagcagcgtc agcagccaat cagcaacagt gaaatggcat
    14041 tttgattatt cgtggcgctt ttgatttcgc tgtctgttta ttttctgaca agcagcgtaa
    14101 actccgcgtc ttcctcttcc agtgatcgac cagcatgcat aactcccccg cagtctccag
    14161 cgcgaaatcg tttgacctga cctcgacggc gtttttaatc gttgcctttc tcaccggtat
    14221 tgcgggcgct ctgcaaaccc cgacactcag tatttttctt accgatgaag tacatgcccg
    14281 tccggcgatg gtgggattct tctttaccgg cagcgctgtc attggggttc tggtgagtca
    14341 gtttctcgcc gggcgctctg ataagcgcgg cgatcgcaaa tcgctgattg tcttttgctg
    14401 cctgttaggc gtgctggcct gcaccctttt tgcctggaat cgcaactact ttgttttgct
    14461 attcgttggc gtctttctta gcagctttgg ctcgaccgct aacccgcaaa tgtttgccct
    14521 tgcccgtgaa catgccgaca aaaccggacg tgaggcggtg atgttcagct cttttttacg
    14581 cgctcaggtt tcactggcat gggtcattgg cccaccgctg gcttatgcct tagcgatggg
    14641 tttcagcttt acggtaatgt atctgagcgc agcggtagca tttattgttt gcggcgtgat
    14701 ggtgtggctg tttttaccgt cgatgcaaaa agagcttccg ctggcgaccg gcacggttga
    14761 agcgccgcgc cgtaaccgtc gcgatacgct gctgctgttt gtcatttgta cattgatgtg
    14821 gggctcgaac agcctgtaca tcatcaacat gccgctattt attatcaacg aactccatct
    14881 tcccgagaaa ctggccggtg tgatgatggg gaccgccgcc gggctggaaa tcccgaccat
    14941 gttgattgcc ggatatttcg ccaaacgtct gggtaagcgt ttcttaatgc gcgttgctgc
    15001 cgtgggtggc gtctgttttt acgcaggaat gctgatggcg cattctcctg tcattctgtt
    15061 gggcttgcag ctgctaaatg ctatttttat tggcattctg ggcggtatcg ggatgctcta
    15121 ttttcaggat ctgatgcccg gtcaggcagg ttcagccacc acgctctata ccaacacgtc
    15181 gcgcgtgggc tggatcatcg cgggatcagt ggcgggcatc gtcgccgaga tctggaatta
    15241 tcacgctgtg ttctggtttg cgatggtgat gattatcgcc actctgtttt gcttactgcg
    15301 gattaaagat gtttaaggcg cggtcagcat ttccagatcg ataagccagg tcattgcctg
    15361 ctgacgcgag ttaccgcaca tttcagcgct ggcttgtaag cctgcacaga ctttcggccg
    15421 cagtggcgag gtgaaaattt tacagcgttg ctgttcatca agctgaatgc agggcgtatt
    15481 ggcgggcttg ccatctggca taccgggaat agggctggaa attgaagggg cagtgcaaca
    15541 cgccccgcaa cccggacggc attccatacg ttgttctctt aaggttaacc accggattga
    15601 tgagcgcgca cagtaccacc ttttttgcac cagcaaaagt gcgaatacca cttgcccgaa
    15661 aggctcgtcg cgagtacttt gtcgcgatat ttttgacatt ttcgactaca ggaatttttc
    15721 gatgccaaga gcgaacgaaa ttaaaaaagg tatggtactg aattacaacg gcaaactgct
    15781 gctggttaag gacattgata ttcagtcgcc cactgcccgc ggtgccgcta cgctgtacaa
    15841 aatgcgtttt tctgatgtcc gtaccgggct gaaagtagaa gagcgtttca aaggagatga
    15901 tatcgttgac accgtgacgc tgacccgccg ttacgttgac ttctcctatg tcgatggcaa
    15961 cgaatatgtc tttatggata aagaagacta taccccgtat accttcacca aagatcagat
    16021 tgaagaagag ttgctgttta tgccagaggg cggcatgccg gacatgcagg tgctgacctg
    16081 ggacggtcaa ctgctggcgc ttgagctgcc gcagaccgtt gatctggaaa tcgttgaaac
    16141 cgcaccaggc atcaaagggg catctgccag cgcccgtaac aaaccggcga cattgagcac
    16201 tggtctggtg attcaggtac cagaatactt aagcccgggc gaaaaaattc gtatccatat
    16261 cgaagaacgc cgttatatgg ggcgtgctga ctaacttcag ccgcatgcag aaaagggata
    16321 gctcaggctg tccctttttt aatttattat accaatcttc tattttgcgc ttcctgaaca
    16381 ccttacgccc tcaattttca ctcgttgatt gatcgccctc acacttcatc gcattaacaa
    16441 tccagaccaa tttcaattgc tgtcatataa ctttacactg tcattgttaa ttaatcgtta
    16501 ctaagacatg actcctatga atacaatcgc ctccgttacg ctcccgcatc atgtacacgc
    16561 tccacgctac gatcgccagc agttgcaatc acgtatcgtt cattttggct ttggagcctt
    16621 tcaccgcgca catcaggcgt tactgaccga tcgtgtgctg aatgcccagg gcggcgactg
    16681 ggggatctgt gaaatcagct tgttcagcgg tgatcaactg atgagccagc tccgcgcaca
    16741 gaaccattta tataccgtgc tggagaaagg ggcggacggc aatcaggcga taattgtcgg
    16801 tgccgttcac gaatgcctga atgcaaaact ggattcctta gcggcaatta ttgagaaatt
    16861 ttgcgagcca caggtggcaa ttgtttccct gacgattacc gaaaaaggct attgtattga
    16921 cccggccacc ggtgcactcg acaccagcaa tccgcggatt attcacgatc tacaaacccc
    16981 tgaagaacct cactccgcac cgggtattct cgtcgaagca ctgaaacgcc gccgtgagcg
    17041 cggccttaca ccgtttaccg tgctctcctg cgacaatatt cccgacaatg gccatgtggt
    17101 gaaaaacgcg gtgctgggaa tggcagaaaa acgttcgcca gaactcgccg ggtggataaa
    17161 agaacacgtc agttttccgg gaaccatggt cgaccgcatt gttccggctg caaccgacga
    17221 atcactggca gaaatcagcc agcatctggg ggtaaatgat ccctgcgcga ttagctgcga
    17281 accgtttatc cagtgggtgg tggaagataa cttcgtcgct gggcgtcctg cctgggaagt
    17341 cgcaggtgta caaatggtga atgatgtcct gccatgggaa gagatgaaac tgcggatgct
    17401 taatggcagc cactcttttc tcgcttatct gggttacctc tcaggattcg cccatatcag
    17461 tgattgcatg caggatcgcg catttcgcca tgccgccaga acattaatgc tggatgagca
    17521 agcgccgaca ctgcggatta aagatgtcga tttaacacaa tatgcggata agttaattgc
    17581 acgttttgct aatccggcgc tgaaacataa gacctggcaa atcgcgatgg atggcagcca
    17641 gaaattaccg caacgcatgc tggcaggtat tcgcatacat ctggggcgcg aaacggactg
    17701 gtcgttgctg gcattaggcg ttgcaggctg gatgcgttac gtcagcggcg ttgatgatgc
    17761 cggaaatgcc attgatgttc gcgatccgct tagcgataaa attcgcgatc ttgttgcggg
    17821 cagcatcagt gagcaacgcg tcaccgccct gctttccctg cgtgaagttt tcggtgatga
    17881 tctgccagat aacccgcatt ttgtgcaggc catcgaacaa gcccggcaac aaatcgcaca
    17941 attcggcgcg catcaggcgc tattaaacac cctcaaaatt taacgatttc tgcggttaaa
    18001 gcggatgaag ctcgccttcg tccgctctcc ccttctcttt tctgccgttt ttagccagga
    18061 ttaacgctca gttaacttac cagagtattg cggagctgtt gtgaccagga ccaacctcat
    18121 caccggtttt ctcggcagcg ggaaaaccac gtcgattctt catctgttag cccataaaga
    18181 tcccaacgaa aaatgggcgg tactggttaa tgaatttggg gaagtcggaa ttgatggtgc
    18241 tttgctcgcc gatagcggcg cactgctgaa agagatcccc ggcggctgca tgtgctgcgt
    18301 taatggttta cccatgcagg tagggttgaa taccttactg cgtcagggaa aaccagaccg
    18361 cttgttgata gagccgactg ggctgggcca tccgaaacag atcctcgatc tgttaaccgc
    18421 accagtctat gaaccgtgga tagatctgcg cgccacgttg tgcattctcg atccacgcct
    18481 gctgctggac gaaaaaagcg ccagcaatga aaacttccgt gaccagctgg ctgccgcaga
    18541 catcattgtc gccaataaat ccgaccgtgc gacacctgaa agcgagcaag cgctacagcg
    18601 ctggtggcag caaaatggtg gcgatcggca attaattcac agtgagcatg ggaaagttgc
    18661 cggtcatctt ctggatttgc cgcgtcacaa tttagccgag ttgcccgcca gcgccgcgca
    18721 ttctcatcag catgtcgtga aaaaagggtt agcagcgtta agcctgccag agcatcaacg
    18781 ctggcgtcgc agtctgaaca gtggacaagg acatcaggcc tgcggctgga tattcgacgc
    18841 tgatacggta ttcgacacca ttggcattct ggaatgggcg cgacttgcac cggtggaacg
    18901 cgtcaaaggc gtgctgcgta ttcccgaagg gctggtgcga atcaaccgtc agggcgatga
    18961 cctgcacatt gaaacgcaaa acgttgcgcc accggacagc cgtattgagc tgatttccag
    19021 cagcgaagct gactggaatg ccttgcagag cgcgctgttg aagcttcgtt tagcgactac
    19081 cgcgtaaggt tgcctgcgtt tttcagtaag ataattagag aaaatatgat taaaaatttg
    19141 ccgcaaatag tgttgttgaa tattgtcggc ctcgcgctgt ttctttcctg gtatattccc
    19201 gttaatcatg gattctggtt gccgattgat gcggatattt tttatttctt taatcagaaa
    19261 ctggtcgaaa gtaaggcctt tttgtggctg gttgcattga ccaacaatcg cgctttcgac
    19321 ggttgttcac tgctggcaat gggaatgttg atgctgagtt tctggctgaa agaaaacgcc
    19381 ccaggcagac gacgtatcgt gattattggt ctggtcatgc tattaactgc agtggtatta
    19441 aaccagctgg gtcaggcatt aattcctgta aaacgggcca gcccaacatt gacttttacc
    19501 gatattaacc gcgtcagcga actgctctct gtccccacga aagatgcctc acgagatagc
    19561 tttcccggcg atcacggcat gatgctgctt attttttcgg cattcatgtg gcgttatttc
    19621 ggcaaagttg caggtcttat cgcccttatt atttttgtgg tttttgcgtt tcccagagta
    19681 atgattggcg cacactggtt tactgacatc attgtcggtt cgatgaccgt gatattgatc
    19741 ggtttgccct gggtgttgct gacgccatta agtgatcgat taatcacctt ttttgacaaa
    19801 tcactaccgg gaaaaaacaa acatttccaa aacaaataac tcacagtact taacatcatc
    19861 aggtttattt ttatagtgag gataatcctg atgatgcgca ccgtgctttc atccatcgaa
    19921 agcaaaaatc attctctaag taaatgaatg gattgcatgc atttcactca atcgtacttt
    19981 aattgaccaa tcccgcttat taactttctg tatcactttt tcttataaaa aatcatgtaa
    20041 aaccgctcgc caagaccgca ccaatcgggt aatctcgaac tcgttttgcc tcggcggtag
    20101 attatcctca cagcatataa ttttgtgcgt tagtccacag atttggcctt aaggaattgt
    20161 ttcaacatgc cctggtaatt agtctcgtgt cgcttggcat tttttttata acgatatttg
    20221 tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga ttttgagata
    20281 tatcttgcgc gggatccccg cgattgcagt agcggttctg ctttctgcat gtagtgcaaa
    20341 taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa catcatcact
    20401 gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa aatcgcgaat
    20461 tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca gcactaaaaa
    20521 aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg gcttagaact
    20581 tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca gtaatttgcg
    20641 tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg gtatttatat
    20701 cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt ccagcatgaa
    20761 tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc gcagctaata
    20821 aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca ctgtttaggc
    20881 agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg atccaggcta
    20941 tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta acggaatcat
    21001 gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg ttgcaggcgt
    21061 aatgattgcg atactggtga gctgccttca gtttttagtg gcctggcata agcacgaagt
    21121 caaatacgac acactgatta ccgacgtaca aaagtatctc gatacctatt ttgccgacct
    21181 gaaatccact actgaccggc tccagccgct gaccttagat acatgcaagc aagctaaccc
    21241 cgaactgacc gcccgcgcgg cgtttagcat gaatgtccga acgtttgtgc tggtgaaaga
    21301 taaaaaaaca ttctgttcat ctgcgaccgg tgagatggac attccactca atgaattgat
    21361 tcctgcgctc gacattaata aaaatgtcga tatggcgatc ttacccggca cgccgatggt
    21421 gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa atagcggcgt
    21481 ctttgccgct ctgaatctca acctgacgcc ttctctcttt tatagctcac ggcaggaaga
    21541 ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt cttcacgttt
    21601 gatgaacgtt aacgaattaa ccgacatgcc agtccgtgaa actaaaattg cgggcattcc
    21661 tctgaccgtt cggctttatg cagatgactg gacatggaac gatgtgtggt acgcattttt
    21721 actgggcggc atgagtggaa ctgtcgttgg cctgctctgc tattacctga tgagcgtacg
    21781 tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt acgtggcgta
    21841 tcaaccggtg gtggatacac aagctttgcg agtaacgggc ctggaagtac tgctacgctg
    21901 gcggcatcct gtcgcgggag aaattccccc ggatgccttc attaactttg ccgaatcgca
    21961 aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg atgccgcaga
    22021 attagaaaaa gtgctgccgg taggcgtcaa atttggcatt aacattgcgc cggaccatct
    22081 gcacagcgaa agctttaaag caaatatcca gaaactgctc acttccctac ccgcacacca
    22141 tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag aagccacaca
    22201 actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact tcggcaccgg
    22261 gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa ttgaccgtgg
    22321 atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg cggtgctgac
    22381 gctggcgaaa cgcctcaata tgctgacggt tgctgagggg gtcgaaacgc cggaacaggc
    22441 gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta gtcgcccgtt
    22501 accgctggac gattttgtcc gctggctgaa gaaaccgtat acgccgcagt ggtaaggtgt
    22561 gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat gatgagtaaa
    22621 aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc tgttcacctt
    22681 tggtgcgcag gcgcaggcta tcaaggaaag ctatgccttt gccgtgctgg gcgaaccccg
    22741 gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa aaggtgggca
    22801 gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac tgcgcggcaa
    22861 cccgggcgca cgcaccgagc agctgtacga cacgctattt acgacttccg atgacgaacc
    22921 aggcagttat tacccgctga ttgctgaaag cgcacgctat gctgacgatt attcctgggt
    22981 ggaggtcgct attaatcctc gcgcccgttt tcatgatggt tcgcccatta ctgcccgcga
    23041 tgtagagttt acttttcaaa aatttatgac tgaaggcgtg ccgcaatttc gtctggtcta
    23101 caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt tagctaaacc
    23161 cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa agtactggaa
    23221 ggatcacaaa cttagcgatc cgctcgccac gcctccgctt gccagtggtc cgtaccgaat
    23281 tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt actgggcagc
    23341 aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg attattacct
    23401 cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc gtatggaaaa
    23461 cgacgccaaa aactgggcca cacgctatag cggtaaaaat ttcgataaaa aatacatcat
    23521 caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt ttaatatcca
    23581 acgtccggta ttcagcgatc gccgggtccg ggaagcaatc accctcgcct ttgactttga
    23641 atggatgaac aaggcgttgt tttacaatgc ctggagtcgc acgaacagtt attttcagaa
    23701 taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc tggcaccaat
    23761 gaaaaaagat ttaccgccag aagtcttcac acaaatctac cagccgccgg tatcgaaagg
    23821 cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg aagcgggctg
    23881 ggtgctgaaa ggtcagcaac gcgttaatgc cactacgggt cagccgctca gctttgaatt
    23941 attgcttcct gcaagcagca atagtcagtg ggtattgccg ttccagcaca gcctgcaacg
    24001 gctgggtatc aacatggaca ttcgcaaggt ggatcactcg caaatcacca accgcatgcg
    24061 cagtcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc ccagttccga
    24121 tttacagatt tcctggtcat cggaatatat caattccact tataatgccc ccggcgtgca
    24181 aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa ataaagaaaa
    24241 attactgccg ttaggacgag cactggatcg cgtattaacg tggaattatt acatgctgcc
    24301 aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc aacccgctgt
    24361 gcgccctgtt tacagtctgg gtatcgatac ctggtggtat gatgttaata aagcggccaa
    24421 actgccgtca gccagacaac agggagagta gatgggcgct tatctgattc gccgtctgtt
    24481 gctggtgatc ccaacgttat gggcgattat cactatcaac tttttcatcg tgcaaattgc
    24541 gcctggcggt ccggtcgacc aggccatcgc cgccattgag tttggtaatg ccggagtatt
    24601 acccggcgca ggcggtgaag gcgttcgcgc cagccatgcg caaacgggcg tcggtaatat
    24661 cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg ccgagatcac
    24721 tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc tctgggacta
    24781 catccgcttt gattttggcg atagcctgtt tcgcagcgcc tcggtgctga cgctgattaa
    24841 agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta tctatctggt
    24901 gtcgattccg ttaggcattc gcaaagctgt ttataatggg agccgctttg acgtctggag
    24961 tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca tcctgctgat
    25021 tgtcttcttc gcaggcggca gctatttcga cctgttccct ctacgcggcc tggtttccgc
    25081 taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc atatcacgct
    25141 gccggtgctg gcgacggtga ttggtggctt tgcggcgctg actatgctga caaaaaactc
    25201 attccttgat gaagtacgca agcaatacgt ggtgaccgcg cgtgcgaaag gggtaagtga
    25261 aaaaaatatt ctctggaaac atgtgttccg caacgccatg ctgctggtga ttgccggttt
    25321 tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg tgatgttttc
    25381 actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt atcctgtaat
    25441 gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg tcagtgatat
    25501 cagctatacg ctggtcgatc cgcgtataga ttttgaggga cgttaatgtc gcgactcaac
    25561 cccgtcaatc aggcccgttg ggcgcgtttt cgccataacc gtcgcggcta ctggtcgtta
    25621 tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc caacgataaa
    25681 ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa ctacagcgaa
    25741 agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg gctgaaacaa
    25801 cggctggaaa ataatggctg ggtactgtgg gcaccgattc gctttggtgc taccagtatc
    25861 aactttgcta ccgataagcc cttcccttct cccccctccc ggcaaaactg gctgggaacg
    25921 gatgccaacg gcggcgatgt gctggcacgt attctctatg gcacgcggat ctcggttctg
    25981 tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg ggcgctacaa
    26041 ggctattacg gcggtaaggt cgatctctgg gggcagcgct ttattgaagt atggtcgggg
    26101 atgccaacgc tgtttttgat tattctgctt tccagcgttg tgcaacctaa cttctggtgg
    26161 ctgctggcaa ttactgtctt atttggctgg atgagtctgg tcggcgtggt gcgggcggag
    26221 tttttacgta cccgcaattt cgactacatc cgcgcggcac aggcgcttgg cgtcagcgat
    26281 cgcagtatta ttctgcgtca tatgttgccg aatgcaatgg tcgctaccct caccttttta
    26341 ccgtttattt tatgtagttc gatcaccacc ctgacctcac tcgatttcct cggcttcggt
    26401 ctgccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa taaccttcag
    26461 gctccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc tttgctgatc
    26521 tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta gcatgacgca
    26581 aactctgtta gcgattgaag atttgtcggt gggttttcgc catcagcaaa ccgtacgtac
    26641 agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc tggtgggtga
    26701 gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc cttccccgcc
    26761 ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc atgccagcga
    26821 ccaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcaag agccgatggt
    26881 gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt cactccaccg
    26941 cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc gcgttggtat
    27001 ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg gcgaacgcca
    27061 gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg ccgatgaacc
    27121 gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc gcgaactgca
    27181 aggcgagttg aatatgggca tgctgtttat tactcataac ctcagcattg tcagaaaact
    27241 ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaatt acgccgctac
    27301 gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg aaccgtcagg
    27361 cgatccagtg ccgttgccag aacctgcctc aacgttgctg gatgttgaac agcttcaggt
    27421 tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg tggtggtgaa
    27481 aaacatcagt tttacgctac gagcgggtga aacactgggt ttagtgggcg agtccggttc
    27541 cgggaaaagc acgacgggac tggcgctgct gcgactgatt aattctcagg gcagcatcat
    27601 ctttgacggt cagccactgc aaaatttaaa tcgccgccag ctgttaccta ttcgtcatcg
    27661 cattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca acgttttgca
    27721 gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac aacgcgaaca
    27781 acaagtgata gacgtgatgc atgaagtggg attagatcct gaaacacgcc accgttatcc
    27841 ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat taattcttaa
    27901 gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg tacaggcgca
    27961 aatattgacg ctattgaaat cattgcaaca aaagcatcaa ctggcctatt tgtttatcag
    28021 ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc gacaagggga
    28081 agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg agtatacgcg
    28141 tcagctactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag ggttcggcaa
    28201 ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg cgatttacaa
    28261 acaggcacgg ctcaccttca cactccagca ccgaacatgg aacttcgatg tcgcctaacg
    28321 cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta agcaatttaa
    28381 gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca accttcgaac
    28441 ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt gagagttgta
    28501 atgaagtcac ttattttccc cggaagcaca tttacttcac aatttgttta catcaatttt
    28561 aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct cgcctgcgtg
    28621 gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc ggggctatat
    28681 gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac tttttacata
    28741 aattgatttt acataaaata aacatatatc gggggaatga tagatttgtg gttgacatgc
    28801 atcaacaaag gaagcctttt agcttcctcg ttgtgcaata gatcaccgtt ttttcggccg
    28861 actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg accaaatcat
    28921 cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg cccctatgcc
    28981 aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa actcatcaag
    29041 aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg ccgcaacgcc
    29101 aaccaccagc gaccaaaatc ccagacccag cagcgcactg atgaccatcc acgctgccat
    29161 aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatac ggcggacgaa
    29221 gcggctatta aagatggtca tcacgaacag gaaaacaatg tttagtgcaa agtaataacc
    29281 aaagttttcc ggcgcgacgt ggttaatttc aatgtagaca aacggtccgg cgcttaagaa
    29341 cgagaacatc ccggcaaagc tgaaaccact ggccagcata taactcagga cacgcttatg
    29401 acggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaaaggct ggcgacgctc
    29461 cggtggtaag gtttctttga tcagaaagaa gatcattgcc gaagccagaa tcgccgccac
    29521 ggcgaggatc cagaagatgt aatgccagct cagccacacc agcacccagc cgccaactat
    29581 cggtgccatc agcggtgcaa tggtcgtcac cagcatgaca aacgacatca tccgcgagaa
    29641 ctcttctttc gggtagatat cacgcatcaa agcgttgata accacgctgg ccgcagccgc
    29701 cgccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg ccaacgcaca
    29761 cgccaccgcg gcggcggcaa acaccagcgt accgccaaga accaccggct tacgcccgaa
    29821 gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa tataagtact
    29881 gagggtcatc tgcgtactgc ccgccggtac gccaaactgc gctgaaatta ccggtagcgc
    29941 gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa ggataaaaac
    30001 aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt cattgaaaag
    30061 ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg gggctaaatc
    30121 agcatccagc gtaatgccgc caatacgttc acgatgcagc tcaaccacat ggttacccac
    30181 ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca gacgaacctg
    30241 cgttggggta atcacttcaa gcaccgctgg cttagtgaga tctttttcgt tatgcagctg
    30301 cacgccttta gcaaattgct ctgccgtatc gtcagctacg ggtgattcca gcgtcaccag
    30361 ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact gaccatcatc
    30421 tgtcattagt accagaccgg tggtatcgat atccagccgc cctgcggcgt gcagtttcca
    30481 cgccactggt tcatctagaa aatagagcac cgttgggtga tcagggtcgt ccgtggagca
    30541 aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct gcgccagcgg
    30601 gttgccatcg taagcgacat catgttcagg aagcagtttg aacgctgcat tacggacgat
    30661 ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag cacggctaac
    30721 gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc ctgtaaaaac
    30781 ggaggtcggg cattgacccg aaaatctgaa ctgttgtctg cccagtatag cggtctaata
    30841 acttccctca agggaaaaag aaccgtggca tactattagc agaataatct acctacgcga
    30901 gaccatgatt tttacactcc gcccatatca gcaagaagcc gtggatgcca cgctcaacca
    30961 ttttcgtcgt cataaaaccc ctgccgttat cgtgctgccc accggtgcag gtaaaagcct
    31021 ggtgatagcg gaacttgcgc ggctggctcg tggtcgcgtg ctggtgctgg cacacgttaa
    31081 agaactggtg gcgcaaaacc atgcaaagta tcaggcgctg gggctggaag ccgatatttt
    31141 tgccgccggg ctaaagcgca aagagagcca cggtaaagtg gtatttggca gcgtgcagtc
    31201 ggtcgcccgt aatcttgatg cctttcaggg tgaattttcg ctgttgattg tcgatgaatg
    31261 tcaccgtatt ggtgacgatg aagagagcca gtatcagcaa atcctcactc acctgacaaa
    31321 agtgaatccc cacttacgcc tgctggggct gactgccacg ccttttcgac tgggcaaagg
    31381 ctggatctac cagttccatt atcacggcat ggtacgcggc gatgagaaag cccttttccg
    31441 tgactgcatt tatgaactgc cgctgcgtta tatgattaaa cacggctatc tgacgccgcc
    31501 agaacgactg gatatgccag tagtgcaata cgatttcagc cgcttgcagg cacagagtaa
    31561 cgggctattc agcgaagccg atctcaaccg cgagctaaaa aaacaacaac gtattacccc
    31621 gcacatcatc agccagatta tggagtttgc tgcaacgcgc aaaggggtga tgattttcgc
    31681 cgccaccgtt gaacacgcaa aagagattgt gggattacta cccgccgaag atgctgcact
    31741 gattactggc gacacccccg gcgctgagcg cgatgtgtta attgaagatt ttaaagccca
    31801 gcgttttcgc tatctggtca acgtcgcggt actgaccacc ggatttgacg ccccgcacgt
    31861 cgatcttatc gccattctgc gccctaccga atcagtgagt ctttaccaac aaattgtcgg
    31921 gcgcggtctg cgtctcgctc ctggcaagac tgattgctta attcttgatt atgcgggtaa
    31981 tcctcacgat ctctacgcgc cggaagttgg tacaccgaaa ggcaaaagtg acaacgttcc
    32041 ggtacaggtt ttctgccctg cctgcggttt tgccaacacc ttttggggga aaacgaccgc
    32101 cgacgggaca ttgattgaac actttggtcg ccgctgtcag ggatggtttg aagatgacga
    32161 cggtcatcgc gaacagtgtg acttccgttt ccgttttaaa aattgcccgc aatgtaacgc
    32221 agaaaatgat attgccgctc gccgctgccg cgaatgtgac acggtactgg ttgatccgga
    32281 tgatatgtta aaagcggcgc tacgactgaa agatgcgctg gtattacgct gtagcggcat
    32341 gtctttgcag catgggcacg acgagaaagg cgaatggttg aaaatcacct attacgatga
    32401 agacggcgcg gatgtgagtg agcgtttccg tctgcaaacg cccgcccagc gaactgcctt
    32461 cgagcagctt tttatccgcc cgcatacgcg cacaccgggc atccctctgc gctggatcac
    32521 cgccgccgat atcctcgccc agcaagcctt attgcgacac ccggattttg tcgttgcccg
    32581 catgaaaggt cagtactggc aagtgcgtga aaaagtgttc gattacgaag gtcgttttcg
    32641 tcgggcgcac gaattacgcg gttaatggta gttttgattg atgtataagg cgattgagta
    32701 tagaatctcg cccgcttttg catacgcaaa gcagatcact tacctgttgc tgggtcgcct
    32761 gtagcaggat taatttaaga gagaaagaaa tgtttactat caacgcagaa gtacgtaaag
    32821 agcagggtaa gggtgcgagc cgccgcctgc gtgccgctaa caagttcccg gcaatcatct
    32881 acggtggcaa agaagcaccg ctggctatcg agctggatca cgacaaagtc atgaacattc
    32941 aagctaaagc tgaattctac agcgaagttc tgaccatcgt tgttgacggt aaagaaatca
    33001 aagttaaagc tcaggacgta cagcgtcacc cgtacaaacc gaagctgcag cacatcgact
    33061 tcgttcgcgc ttaattgctg aataagttgt aaaaaacccc gctccggcgg ggttttttgt
    33121 atctgcagat tatgcctgat tacgatattg ctattttttg caggccagat aaggcgttca
    33181 cgccgcatct ggcattcata acacaaaacc gattaattgc cgccggacgt ccggcgctgc
    33241 aattgatcgc gcaaattcgg cggcgtgcct ttaatagtca gggtatcggt cgccggatcc
    33301 cagaaaatcc gttcgcccaa cagcatcgca tcaaaattaa tagtcaagcc accgccgcta
    33361 ccggcaaact tcgtcaactg acgcagtgtg ctacggtctg ccgggaagct ctcttccagt
    33421 tcgtagcctt tttcagccgc aaactccgtg aagctcactt cgctgacgcc tgccagctct
    33481 ttcgacagcg attccagttc gatctcttcg cctgcctgca gctgctcatt acagtagcta
    33541 taaacctgct ggcgcacgtt ctgtcgctct gctttatcca gttgtgcttc ggcagtgaaa
    33601 tcatcaaccg cttgcaacag cccacggttt tgcgctttcg cgttcaatcc ttcgctggcc
    33661 ccgaggaaat ccataaagaa atccgccact ttgcgcccta ctcgcccttt caggaaagtg
    33721 agatagcggg tcgactctgg attggtttcc cattcggtta aatcgatacg cgcaacaata
    33781 tccgcatgat tgatatcaag ataatgggtt gggttgatgt cgagattttc gttaacgcgc
    33841 atactgctca ggttgctcag gactgccacc agcagatact ccaccgccag ataacggtag
    33901 tggcaaaaca gcacgaatcc accgtcagca aaaggatatt tcgctaattc gtcacgcagg
    33961 cgaccggttg ccgcgcggct aaatgccaga aagtcctctt caccctgacg ctgtaagcgc
    34021 agcgtctgcg ccagttcact ctcttcgcta aacaagccgt aagctttatt tttggcgcta
    34081 tagacccgat gcagttctgc taccatctca acgacggttt ctgtcggttc cagcaatgaa
    34141 tcgcgcaaca ccagctcaag gttctgctca tcacgcttga taagctggtg cagagcaatc
    34201 tggttgatat ccagactcat gataaactct cctttaagac cgggcggtat tcaaccaccg
    34261 ccggttaagc gacgcaataa aagatggcgg tcattttaca gaaggcggcg taacgagaat
    34321 gatttaacgc ataaaaaagc agaaaaaaaa ccgttgctac ggtaatatgt tgccctttca
    34381 tcaactaact gatttcgatt tatgccacaa atttcccgct acagtgatga acaggttgaa
    34441 caattgctcg cagagctgct caacgtactg gaaaaacata aggctccgac cgacctttcc
    34501 ctgatggtgt taggcaacat ggtcactaac cttatcaaca ccagcattgc cccggcccaa
    34561 cgccaggcaa ttgccaactc ttttgcccgc gccttacagt cctctatcaa cgaagacaaa
    34621 gcgcactaag ggaaacagat aacaggttat ggtaactcat cgtcagcgct accgtgaaaa
    34681 agtctcccag atggtcagtt gggggcactg gtttgcactg ttcaatattc tgctttcgct
    34741 cgtcattggc agccgttacc tgtttatcgc cgactggccg accacgcttg ctggtcgcat
    34801 ttattcctac gtaagcatta tcggccattt cagcttcctg gtgttcgcca cctacttgct
    34861 gattctcttc ccgctgacct ttatcgtcgg ctcccagagg ctgatgaggt ttttgtccgt
    34921 cattctggca acggcgggaa tgacgctatt actgatcgat agcgaagtct ttactcgttt
    34981 ccatctccat cttaatccca tcgtctggca actggttatt aacccagacg aaaatgagat
    35041 ggcgcgcgac tggcagctga tgttcatcag cgtgccggtt attttattgc ttgaactggt
    35101 gtttgcgacg tggagctggc aaaagctgcg cagcctgacg cgtcgtcggc gcttcgcgcg
    35161 tccgctggcc gcattcttat ttatcgcctt tatcgcctcg catgtggtgt atatctgggc
    35221 cgatgccaac ttctatcgcc ctatcaccat gcagcgcgct aacctgccgc tttcgtaccc
    35281 gatgacggcg cgacgttttc ttgagaagca tggcctgctt gatgcgcagg agtatcaacg
    35341 ccgtcttatt gagcaaggta atccagacgc cgtttccgtt cagtatccgt taagcgaact
    35401 gcgctatcgc gatatgggca ccgggcagaa tgtcttgttg attactgtcg atggcctgaa
    35461 ctactcacgc ttcgagaagc agatgcctgc gctggcaggt tttgctgagc aaaatatttc
    35521 gttcacgcgc catatgagct ccggcaacac tacagacaac ggcatctttg gcctgttcta
    35581 tggcatctcg ccgagctata tggacggcat tctgtcgacc cgtacgcctg cggcattaat
    35641 tactgcgctt aatcagcaag gctatcagct ggggttattc tcatcagatg gctttaccag
    35701 cccgctgtat cgccaggcat tgttgtcaga tttctcgatg ccgagcgtac gcacccaatc
    35761 cgacgagcag accgccacgc agtggatcaa ctggctggga cgctacgcac aagaagataa
    35821 ccgctggttc tcgtgggtct ctttcaatgg tactaacatt gacgacagca atcagcaggc
    35881 atttgcacgg aaatatagcc gggcggcagg caatgtcgat gaccagatca accgcgtgct
    35941 caatgcactg cgtgattctg gcaaactgga caatacggtg gtgattatca ctgccggtcg
    36001 gggtattcca ctgagcgaag aggaagaaac ctttgactgg tcccacggtc atctgcaggt
    36061 gccattagtg attcactggc caggcacgcc ggcgcagcgt attaatgcgc tgactgatca
    36121 taccgatctg atgacgacgc tgatgcaacg cctgctacat gtcagcacac ctgccagcga
    36181 atattcgcaa ggtcaggatt tgttcaaccc tcaacgccgt cattactggg ttaccgcagc
    36241 ggataacgat acgctggcaa ttaccacccc gaaaaagacg ctggtgctga acaataacgg
    36301 taaataccgc acttacaact tacgtggtga aagagtgaaa gatgaaaaac cacagttaag
    36361 tttgttattg caagtactga cagacgagaa gcgttttatc gctaactgat taattataaa
    36421 tcagttagcg aaatatctta cttgcaatcg gtgtggaaaa cggtagtatt aacaaccacg
    36481 agtcggcacg tagcgcagcc tggtagcgca ccgtcatggg gtgtcggggg tcggaggttc
    36541 aaatcctctc gtgccgacca aaaatcccaa aaaaaccaac cgtaaaggtt ggttttttta
    36601 tatctgcatt taattcgata aacagaccgc gacacatcag ggcctgttta ttgtctgtta
    36661 tcagaacgtc cagaccacac ccgcctgggt attccacggc gattctaccc ctgcgccatt
    36721 gccatagctg actgacaaat gaccgcttaa cgtcggggta aacgatgacc ttatcccagc
    36781 ctgataaacg ccacgggttc cagccacatc attattaaat ttgccatcgt cattaacttt
    36841 cacctggtta gaatcggcgt attcctgacg cacggcggct ttcagccagg gttccagcgt
    36901 cgtaccgttt tgcaggtcca tgtgatagct taccgccgtt cccgcttcag cgcgtaatat
    36961 ccgggtattt cccacatccg cgcgcatgcc gtttgataac gtgtagtcct gaccatctgt
    37021 ggtaaagccg gtaaaggcca gatagggtct aacactccac aatccgtcaa cccaacggaa
    37081 cccgctctcg acatgagcac ccgcgccgtt actattgtaa tcgccaaacg ctgttgcccc
    37141 attactcatc ttgccatgga tggtgttggc aaaacggtca actttcacca ctccatcaac
    37201 ataggcaccg ttctgatgct cccaaccggc ataagccccc agggtatagc tatcgacatt
    37261 gcctttgccg ccgcgatcaa aaccaatatc agaatgagag taaccaaaga acaagccgcg
    37321 aattgtgctg ctttcttcac gggagaaacg gctatcgata ccgagcgtca ggcccgtcaa
    37381 tgtttgctca aaaccagctc ccgcatcagt ggtcacgttg ttgcgggtgt taattgccga
    37441 actccacatc gccgtatcgt aactaacgcc ttttacgcta ccaagacgct cacgcacggt
    37501 gtccagttct gcatcaaata ccagcggttg tgcggccgcc atattcagca catcagtggt
    37561 tgaaggggta atttgcgcgc gattctctgc cagactccag ctatggttac cattatccag
    37621 caaggtatat tcatacgtac cgatatcaac aacgcctccg gcattgccca acgtaaatgc
    37681 agcatcaccg ccgcccgttg ttaccagtgt aaggctatct cctgctgccg ggctggcacc
    37741 ggtgtccgtc acgaatattt tgaaatcacc tgttgcctga ccggtgacgt tgagctgatc
    37801 gctctgatga ttcgccatat cggtacgcat ataaaaattg ccgtttcccg acagggtatt
    37861 ggtggtcaac gtagtgaaat tttccgcact tgccgtcaca gatgaacgag tcactgactc
    37921 atccatcata atcacatttg cgccagacat cgcaaggttg gctaagttaa ggcgggtgtt
    37981 ttggttcgtc gtagtgatat cggcaatgat ggtccatgcg ctattttcga gcgaaacatc
    38041 cgctttgctg gtatccacgg caccatgcgt tcttaaagaa gcgccctcag aaaccactac
    38101 cttacccacc aacattgcgg gtgcataatt tatttgcggc ttcataacct caagaatgcc
    38161 atcatttcct ctgactgaca cgttaaccat tgtgccagcc cagacgttag cgcggccatt
    38221 gttgataacc atattttcag agatggcttt agaggaatac gttatacttc cattctgatc
    38281 tgatcgaccg agatcatata ccgcatttgc ctgcactctc gtaccagtat cctttcccag
    38341 cgattgcata gtggcatgct catcgaggat agtgtcaatg gcctgcgtgt cctccataac
    38401 aataagcccg gaaccatcat ccagttcata attttttgac acaccatctt ttatactaaa
    38461 ttggccttta ctgtttgtac cactcacttc cagcgcattc gttgagacaa ttaattttcc
    38521 gcctgctttt ttatcaacgc cagttgcagt cccgccatcc ataacctcca gtaagccgcc
    38581 actatctaca gtagtattat aagcgaagtc attttcttca actcgcaaac ttccgccgtt
    38641 ttccaacagc atattgttag caataccatt cttgatttcg aactgaccga gacggtttgt
    38701 tccgaatacc tccatgaccc gcgtgcttgc tttaatagca ccgcctgctt tctgatctac
    38761 cgcaatcgct aatcccccct ctttaacctc cagtaatcct cccttttcga tagcggcaag
    38821 gacggtaatc ccatcttttt caacgattaa gcgcccgcct ttaatcgtgg gatcattcgc
    38881 tgcgccataa acctgtagca caccattatt gagcgtagtc tggtttgccg cgccacccat
    38941 ttgaacaatt tgataaccat catttaatac tgagtattct gcggtgccat tcatttcaat
    39001 gtactgatac ccgccgttaa tttcagtatt actacttata ccaaattctt taatatgctg
    39061 ttctccgcca ggattgattt tcgtattatt ggcggtccca taaaccagct ggatatcttt
    39121 ttccaggaca acaccatcaa ctgtctcacc atactcaact gttgatgcca atgctatggg
    39181 tgaatataac gctgtagcta ccagaacaga tattgcggaa cgacaaagag aaacagaacc
    39241 agattgatgc attgagcttt catcctatga aattaattgc agttaaaagc attgggtaca
    39301 gaaaataccc atagctccat acccggagtc agtttttaaa aactgtttaa agaaatgcac
    39361 aagtattgtg attgattttt tagttgtttt tcttgatgag aagctgatgc aaaattccgt
    39421 ctttataatg aaaatgatgc caaagcgaac gacaaggttg tagttttcgc tacatgtcca
    39481 tacataaaat ggggtaacat tcacgcgcct ggtagcgtta ccaacgctac gctcaaacat
    39541 aatgattcta ataaaacctc aggagactac tatgcctgaa gcaacgcctt ttcaggtgat
    39601 gattgtggat gatcatccac ttatgcgacg cggtgttcgt cagttactgg agcttgatcc
    39661 tggctttgaa gtggtcgccg aagcgggcga cggcgcgagt gctatcgatc tggcgaatag
    39721 actggatatc gacgtgatct tgctggatct caatatgaaa ggtatgagtg gcctggatac
    39781 cctcaatgcc ttgcgcaggg atggcgttac cgcgcaaatt attatcctga ccgtatcaga
    39841 tgcctccagc gatgtctttg cactgataga cgcaggcgca gacggttatc tgttgaaaga
    39901 cagcgacccg gaagtgttgc tggaagcgat tcgtgccgga gcgaaaggca gcaaagtctt
    39961 tagcgaacgc gtcaatcagt acttacatga acgtgaaatg tttggcgcgg aagaagatcc
    40021 cttcagcgtg ctgacggagc gcgagctgga tgttctgcac gagctggcac aggggctgtc
    40081 aaataaacag attgcctcgg tgttgaatat ttccgagcag acagtaaaag tacatattcg
    40141 caatctactg cgcaaactca atgtccgctc acgcgtggca gccaccattc tgttcctgca
    40201 acaacgcggg gcacaataaa aatagcccga tggcatcacc atcgggcctc tttttattta
    40261 ctctcctgcg gcgacaaatg ttgcatcgcc tgcgcgatac tacgttcaat caccgcacgg
    40321 cgagtatcgt tggcaggtaa gagtttcaac atcatctccc acgcggcaac ggcttcgcca
    40381 aatcgctgct gctcaaaggc attaaacgca tacatgctta acacacgaat attgctatgg
    40441 tccgttctca ccaactggcg cagcagttcg ccgccgaggc ggttgtcgtt gggatcagat
    40501 gaacgtgtca acgcttcagc ataacccagt gtggcatcgc tgtttttcgg atcgaggcga
    40561 tacgcagtag cgtatgcatc ggtggcgata ctggcgttac ccagcgccat gccaacgcga
    40621 cccaacataa tccaaccttc tatatctccc ggattttttt gcagttgagt acgcatcccc
    40681 agcgcaaggc gcgacatctc ttcttcgttg agcggatcgg ctttcggatc cagcgccctg
    40741 tccagcaacg ccggagcctg tgccgtggcc tgctgccaga ttttcacctg ctgataattg
    40801 ccagtctggt agtagctgac gccagccaca attaacgcca ccacaatacc cggcagataa
    40861 acaacatatc cggcacgctt accttccggc acgctttgtt caggaaacgc ttccggtacc
    40921 acgcgtaccc gacgccgcga acgggcgtat atgacccaac cgccaatacc aatagccact
    40981 accggaagca cccacagcag cacggtcagc ggcgttaacg gcggatcgta agtgacgaag
    41041 ttgccgtaac gcgccaccat ataatcgaca atctctttct tacttttgcc ttcctgcatc
    41101 agttcataca ctttctggcg caggtcggtg gcaatcatcg agttggaatc ggcaatgctg
    41161 ttattctggc atttcgggca gcgcagttct tcagtgagct gacggaactg ttgttcctgt
    41221 gcttcatctt taaactgcaa cacgtcgatg gtcgccagcg ctgagccgga gatcatcagc
    41281 atcagcacgc ccaataaaaa cctcattgtg cggcctcctt actgtatttc tcccacagcg
    41341 gcttgatctc ttcttcccag acgcgaggat tgagatcgcc cgcatggcga tagcgaatga
    41401 tgccgttgcc gtcaataaga aacgtttctg gcgcgccata gacaccgaga tccagcccta
    41461 acatgccatc gccatcaaaa aggcttagcg cgtaaggatt gcccagctct ttcagccagc
    41521 tgattgcctt ctggcgatcg tctttatagt tcatgccgac cacgcggatg ccctgcgcag
    41581 aaagctgatt cagatattga tgttccgcac ggcaggtcgg acaccaggtc gcccagacgt
    41641 taagcagcac tggtttgccc tgagtcagca catccgcctg ataaaactgc cccggattgt
    41701 ccagtgattc gagacgaaac ttcggcacag gcttgccaat gagcgccgat tccagattgg
    41761 tcggatcatc cccttcggca ttacgcgcca gctgccacag cagcgccgcg gcaatcgcca
    41821 ggaagataat caacggaatt aacaatactt tgcgcttcat acagcctccg gcgcagtttt
    41881 ttgcgggctc acgcgcttac gatagcgagg atcaaacaga cacagcagtc cgcccaacgc
    41941 catcatcagc ccgcccgccc aaatccagcg aacaaatggt ttgtagtaaa gacgcacagc
    42001 ccacgcgccg ttttccagct cttcaccgag cgcggcgtac aggtcacgcg tgatgccgcc
    42061 gtcaattgcc gcttcggtca tcatcgaccc ggcagtgttg taataacgtt tttccgcata
    42121 cagcaccgtt tccggtttac cctctcgcgt tacgccgata gtcgccacac cgccacgcca
    42181 gttcgggcca gtcacctctt tgacatcacg gaaggtgaag cgatattcat gaatatcgac
    42241 gctatcgccg gacttcatgc gcacatcacg ctcaacgcta tagttctggc taaaggcaat
    42301 gccaacaatt gtcactgcca gccccaggtg agccgccacc atcccccaat aactgaaggt
    42361 ggttttcgtg ccgcgtgaaa tacgtagcgc agcttccgca attgccagca ccgcaatcca
    42421 gcaggccatt gccaggccga gcaccgtcat cgccacaact ttgctttcga acagccacgg
    42481 caacagcaga gacagcacca gcgtagagat gaaggcgata atcaataaat tgcggatttt
    42541 acgtgggcga tcgcgccccc agcgcaccag aggaccgaca cccagcagta gcgcaaacgg
    42601 caccatcagc caggtaaaca tggtgttgaa gaacggttcg ccaatcgaaa tactgcccag
    42661 tcccagttgc ttgtgcacca gcggcagcag cgtccccagc aacaccacca gcatcgcggc
    42721 gaccagcaaa acgttgttcg ctaacagcaa agattcccgc gaccacagcg cattgtttac
    42781 gcgtgagcga actttgtgtc cacgcgcggc aaacagcagc agcgaaccgc caatcaccag
    42841 caccataaag gcgaggataa acataccgcg cgccggatca gacgcaaacg cgtgtaccga
    42901 taccagcacg ccggaacgca cgaggaaagt ccccaacaga cacaacgaga aggcactgat
    42961 tgccagcagt aatgtccacg ctttgaagct ggcgcgttgt tcagtgaccg ccagtgagtg
    43021 catcagcgca gtccccacca gccacggcat aaacgaggcg ttttctaccg gatcccagaa
    43081 ccaccagcca ccccagccga gttcgtaata ggcccatgcg gaaccgagca cgatgccgag
    43141 cgtcaggaag atccacgctg ccagcgtcca cggacgagta aaacgcgcat aagtgctgtc
    43201 cagacgcccg ctcagcaaag aagcaatggc aaaagcaaac gccaccgaga aacccacgta
    43261 ccccatataa agcagaggcg gatggaagat cagccccgga tcctgtaaca gcgggttaag
    43321 atcgcgccct tcaatcggga agttcggcag cgtgcgtgag aatgggttag aggtaaagag
    43381 aatgaacagc aaaaagccga cactgaccat ccccattatc gccagtacgc gggccacaat
    43441 atccagcgga atacgctgac taaaaatcgc caccgcaaag gtccagccgc tcatcagcag
    43501 cacccacagc agcagtgagc cttcatgtgc gccccaggta gccgccacgc gataccacac
    43561 cggaagctgg gtattggagt tgctggcaac ataggtgacg gtaaagtcgt tgaccacgaa
    43621 agcattgacc agcaccagaa atgcgccagc cacagacata aacagcagcc aggcaaacaa
    43681 gcgggaagac gccatcatgc gcgcatctcc gcgcgccacg ccccatagcg gatacacgga
    43741 cagcagcagc gcaattccca gcgccaggca cagcagcccg ttaccaattt ccggcatcat
    43801 gatgctgggt ccttataaac actcgccggg cgacggtggt tagcttccat cgctttctca
    43861 acttctggcg gcgtgtagtt ttcatcatgt ttcgccagca cttcttttgc gaggatatga
    43921 ttgccttttt ccagctcgcc ctgcaccaca acgccctgcc cttcacggaa cagatccggc
    43981 aaaatgcctt cgtaagagac atccactgag ccttcagcgt cgtaaatggt gaaggtcact
    44041 ttcagcgaat tgggatcgcg ctgcacacta cccggcatca ccatcccgcc aacgcgcaga
    44101 cgctgaccga cttccggcat ctgctgagtt tcacgcttgc cgtagagaat ttcccccggc
    44161 gtataaaaga gatcgatatt cgagcgcagc gcatatagca ccagaccgat agtcagcgcc
    44221 agccctgcca acacggcaca ggcaatccac aagcggtttt tacggcgaat attcatgcag
    44281 cctcctgctg ttgcgcagca cgtaaacgcg cctcacgcgc ccgctgttgc gccacaccac
    44341 gcagaattgc gcgatgctgc atcaccgagt gcacgaccaa aaccaccagc ggaataacgg
    44401 tcatcaccac cgccagccag acaaaaaagg cgtaaccgcc cattgcgaaa aattcattcc
    44461 aggaagcaaa tgcaggggtc atttacggcc tcttttcagt atcagttcac tcacccacgg
    44521 acggcgtttt tccatcagca aaatcaaatt acgcatccgc atcagcgtca gcgtggcaga
    44581 gaggagcagg aagccaaaaa tcgaccagcg cagcggcgaa cgcatcgccg gatcgatact
    44641 ttgctgcatc cgcgttgatc cctgatgcag ggtgttccac cactccacgg agtaatgaat
    44701 aatcggcaga ttcaccacgc caatcagcac caggatacct gccgcacggc ccgccagacg
    44761 gcggtcgtcg aaggcgtgcc acagggcaat cacacccaca tacaaaaaca gcagcaccag
    44821 ttcagaagtc agacgtgcat cccataccca ccaggtgccc cacatcggtt ttccccatgc
    44881 agagccggta accagggcaa taaaggtaaa cacggcacca atgggggcca tcgccgccac
    44941 cgccaggttg gccattttca tctgccagac aaggccaata aacgccgcca ccgccattga
    45001 tgcataaatg cccatcgacc agatcgccgc aggcacatgc aggtagataa tgcggtagct
    45061 atttccctgc tgataatcag ccggagcaaa gccgaatccc cagatccagc cgacggtaag
    45121 cacgactaca ctggcaattg ccagccacgg tataaaccag ccacagattt gatacagccg
    45181 tggtgggatc gccagttgat gcagtgtttt ccacatagtt tcgataccag actcgaacaa
    45241 aaatcagtaa tccagcgtta ttgaatgctg attcgtaacg ctgccgccgt cgcaaaagga
    45301 cttaatgtcg cggtgcctgc cagcaacgcg cctaaaattg ccagataccc gtcaacgggc
    45361 aaatgcatag aagccgcgtc catcgcggcg gtggcaaaga tgagtaatgg gatagtcagc
    45421 ggtaacacca gtatgctgag cagcacacca ccgcgcttaa gtcccactgt cagcgccacg
    45481 cccggtgcac cgagaaagcc aagcgtaggc gttcccagca gtagcgttaa cgccatcact
    45541 tgccagccat aaacatccat tcccagtagc attgctacca gtggcgaaag gatgagtaac
    45601 ggcagaccgg ttaccatcca gtgcgccatc accttcgcca gcacaacggc gggcaagggt
    45661 aacggcaaca acatcaattg ttcaagactg ccgtcctgca aatcgtcacg gaacagtcgt
    45721 tccagcgcca gcaaggatga aagcagcgcg gcaacccaga taatgcccgg tgcaatacgt
    45781 gccagcagtt gcggctccgg accgatactg agcggaaaaa gggtaattac aatcaggaag
    45841 aaccacagcg ggttggcgat ttcagcgcta tggcgaaacg ctacgcgcag ctcaagacgg
    45901 aaaatgcgcc agaacatcat acggccctcg tttgcgtcag tgaaatgcgg cgaattttac
    45961 tttcagcaac gttgagcggc tggtgggtag tgagaatcac aatccccccc tgctctgtat
    46021 gctgcgccat acgctgggtc agacgatcga cgccgttaac gtcaatcgcg gtaaaaggct
    46081 cgtcgaggat ccataacgtg gcacgggtca gccacagacg cgctaaagcg acgcggcgtt
    46141 gttgcccggc agaaagttga tttaccggaa tatcttcgaa tccggcaagc ccggcctgcg
    46201 ccagggcttc cagacattgt gcggtatcgc catcgcgatg ataaaagtgc agattttcta
    46261 acgccgtcag ccgggttttg atccccggct gatggcctat ccataacagg ttttgatggt
    46321 agctgtcgcg tacctgatgc aagggctgcc cttgccagag aacctcgcct gcgtcagggc
    46381 gagacaaccc cgtcagcaaa cggagaagcg ttgtcttccc cgcgccgttg ctaccggtga
    46441 tttgtaccca ctctcctgcg ttcagcgtaa atgacaagcc actaaataag gttcgttcat
    46501 cccgctcaca aagtaactct ctgacttcaa gcatacccac gcaataaccc tgttaaaaac
    46561 ctggctcgac ttcacgcata tcgggcagct tgtgcgctat ccctttatgg caatcaatac
    46621 aggtttgccc atctttcacc gcctggtcat gcatcttcgc ggcaaccgat ttctgggcgg
    46681 ttgtatccat atactcgaag ttgtgacagt tacggcactc ctgcgagtta ttgtccttca
    46741 tgcgccgcca ctcattctgt gccatcgtca gacgatgagc ttcaaatttc tgcggcgtgt
    46801 caataacgcc aaaaatttta ccatacagct ctttacttgc tttgagcttg cgtatcatct
    46861 tcggcacaaa ctcgtgcgga acgtgacaat ccggacaggt cgcacggacg ccgctacggt
    46921 tgttgtagtg cacggaatcc atgtattcct gatacaccgt gttacgcatt tcgtggcagc
    46981 taatgcagaa ctcttcggta ttggcttttt ccatcccagt gttaaagccg ccccagaaga
    47041 cgatgccgcc gacaaaaccg atcaacagca gcgtccccag cgccagacgg ctgggggtac
    47101 gccaccattt ccacaggcgc ttaatcagac caggcttacg gtcagaattt cccataataa
    47161 cctcttattt cccgtaacct tttgatgggg taaaggtatt ccccacgatt ggcgcggtat
    47221 cggcctgcgg tacgtgacat tgcagacaga aataacgacg cggagccact tccgcgccca
    47281 ctttgccgtc gctgtccata aagtgagtag gactgatacg cggcgcgcca gtggtgcgat
    47341 agctttcgac accgtggcac tgcaagcagc ggttggtatt ggtcgttacc tgataacctt
    47401 caacgctatg cgggatcatt ggcggctgat tcacatagtt cagcggcatc cgatcctgct
    47461 cttttggcat ccgaatggcc ccttcctgcg tcccggatac ttccggcgac tggctaaaat
    47521 cgacgccatt agccgcccaa accgcgccgc ttaccaccag ggccagcatc gccgtccatt
    47581 gacacagcgc tttcttcagg tcatggcttt tcatgatttc gctcccgaac tccatcgtgt
    47641 agttattgta aatacatcct cagaacagac atccacgcag cgaccgcacg tcatgcaatc
    47701 gcggctggtg acctgcaccg ggctttgttc atccagcacc ggggcacgta gcacatgcgg
    47761 ttccgggcaa acatgaaaac aatccataca gcggttacat ttctgccgat cgctggccgc
    47821 aacggtaatc acacctttgc tacccagcac gccatacagc gcgccaaccg gacaaatgtg
    47881 cccgcaccag ccgtgttcaa cgaccagtag atcaaataaa aacagcgcga gaataagcag
    47941 cgcgccgctg ccgaagccca ttaccaggct acgccccatc agggaaacgg ggttgatcca
    48001 ttcccagatg agcgtgccag ttaaggctga tcccaccaga atgaccacca acagcacgta
    48061 gcgaatgtgg cgagggatcg tcgcagactg attgaggtca aagcgcctgc gtaaccagtt
    48121 cgctaagtcg gtaatcgggt tcagcgggca gacccagctg caaaataatc gctttcctgc
    48181 cagggcgtag agcacggtga taatcaccgc accggtcaac gccaccgtgg caggcagatg
    48241 accactggca agactttgca gcgtcatcag cggatcggtc agcggaacgg tgtcgaataa
    48301 caggctactg ctatagttgc cgtgcaagat ccacacacca aaccacggac cgctcaaaaa
    48361 catccccagc acgaagaact gacaaaggcg acgtaacacc agccaacggt gactgcgcca
    48421 ccagcctttt ttctccagcg cctcgcgccc ggcgtcacgt ttacgatttg ccattgttcc
    48481 cctccagcca gccgaagcgg taatggtgac ctaactcccc tttcgccagt gacagcggta
    48541 acaccttgat tgccggttgt tccagcacgc atactttttc gcacttaccg caaccggtac
    48601 aggcgtcgct gtgaaccgtc ggcagaaagc gggcgtgctt accggtacgc gtgttgcgct
    48661 ccagctccag ggtgatggcc tcatcaattt tcgggcattc gcgataacaa acatcgcagc
    48721 gcagcccctg aaagttgaga cagttttcct ggtccaccag taccaccagc cccatccgcg
    48781 cgtcgtcgat cgattcaatc tcacgatcca gcgcaccgct cgggcacact ttggcgcacg
    48841 gaatgtcctc acacatttcg caaggaatat cccgtgcgac aaaatatggc gtgcccgccg
    48901 acaggccaga ggccagcgtc gccagtttta aggtgtcgta agggcaagcc tgaacacact
    48961 gaccgcaacg cacacaggca ctggcaaagg cgttctcgtt tatggctccg ggcgggcgca
    49021 accgcacgcc agatgcgcgt gcggtttgct gttgtaaccc cagcgccaca cccacggcag
    49081 ccagcccgcc tgctgtgcga acgacatcgc gcagaaagcg gcggcgacca ttttgaggtt
    49141 tcgctgaccg ggacataatg gcttacacct tctccagttt gaccgcgcac ttcttgaagt
    49201 ccgtctcttt cgagagcgga tcggtcgcgt ccagcgttag tttgttaacc agctgtgcgg
    49261 cgtcgaagaa cggcatgtac accaggccct gcggcggacg gttacgaccg cgcgtttcga
    49321 caatcgagat cacttcgcca cggcgagaaa ccactttcac tttgtcgcca cggcgcagat
    49381 cgcgcgcttt cgcatccagc gggtgaataa acaggaccgc ttccgggaag gcacggtgca
    49441 gttccggcac acggcgagtc atactgccgg tgtgccagtg ctccaggacg cgtccggtag
    49501 agagccacag gtcgtactct tcatccggtg cttccgccgc cggttcgaac ggcagcgcga
    49561 agatcaccgc tttaccatcc ggtttaccgt agaacttata gccttcgccc gctttcacgt
    49621 acgggtcgtt accttcgctg taacgccact gcgtttcttt accgttcacc accggccagc
    49681 gcagaccgcg cgctttgtgg tagtcatcga acggcgcgag atcgtgaccg tgaccgcgac
    49741 caaaccatgc gtactcttcg aacagccctt tttgcagata gaagcccagc tcgcgggatt
    49801 catcgttcag ctgatcttcc gccagttcgg ataccgggaa tttgctcact tcgggggtgg
    49861 catacagaac ttcgtacagc gttttgccac gcagttccgg tttcttcgcc agcagctctt
    49921 ccggccatac ttcttcagtt ttgaagcggc gggagaactg gactaactgc cagaggtccg
    49981 atttcgcttc gcctggcgcc tgtacctgct gacgccagaa ctgagtacgg cgttcggcgt
    50041 taccgtaagc cccctctttt tctacccaca ttgcggtcgg caggatcaag tcggcggcca
    50101 gcgcactgac tgtcggatac ggatcggaga cgatgatgaa gttgcgcgga tcgcgccagc
    50161 ccggcatacg ctcttcatta atgttcggcc cggcctgcat gttgttggta cacatggtcc
    50221 agtaaacatt cagcttgccg tctttcagcg cacggtcttg tgctaccgca tgcagaccga
    50281 ttttcgccgg aatggtgccg ctcgggatat tccacttctt ctcgcagata tcacgatgtt
    50341 tctcgttagt taccaccatg tccgcaggca gacggtgagc aaaggtgccc acttcacgcg
    50401 cagtaccaca cgcggaaggc tgcccggtca gggagaacgg accgcaaccc ggctgggaaa
    50461 ttttgccggt cagcaggtgc aggttgtaga ccaggttgtt ggcccacacg ccacgagtat
    50521 gctggttgaa gcccatcgtc cagtaggaga tgactttctt gttcggatcg gcatacagct
    50581 gcgccagttg ttccagctga tcttttggta cgccagtcat ttcagcggtt ttttccagcg
    50641 tatactcagc aacgaaggct ttgtaatctt caaagctcat cggttcggag gcgtcagaac
    50701 ccggattctt cgctgctttt tccagcggat gggtcggacg taaaccgtag ccgatgtccg
    50761 tcgccccttt gcgcaggtta acgtgcttgc tgaagaagtc ctgatttatc gcattgtttt
    50821 gaatgatata gttggcgatg tagttcagga tcaccaggtc agattgcggc gtaaagatga
    50881 tgccgttatc cgccagctcg aagctacgat gctggtaggt tgaaagcacc gccacggtga
    50941 cgtcctggtt agagagacga cggttagtga tgcgtgacca gaggatcggg tgcatctccg
    51001 ccatgtttgc gccccacagc acaaacgcgt cagcctgctc gatgtcgtca tagcagccca
    51061 tcggctcatc cataccaaag gttcgcataa agccaactac tgccgacgcc atacagtgac
    51121 gcgcattcgg gtcgatgttg ttcgaacgga agcccgcttt aaacagcttg gacgcggcat
    51181 aaccttccca gatagtccac tgaccagaac cgaacatacc gatcgattcc ggcccttttt
    51241 ctttcagggc ggttttgaat ttctcttcca tcacatcgaa ggcctgatcc caggtgattg
    51301 gggtaaattc gccttctttg tcatatttac cgtttttcat acgcagcagc ggctgcgtca
    51361 aacggtcttt accgtacatg atcttgggca ggaaatagcc cttaatgcag ttcaggccac
    51421 ggttaaccgg tgcgtccggg tcgccctgac aggccaccac acgcccctgc tgcgttccga
    51481 ccagaacgcc gcaaccagta ccgcagaaac ggcacggcgc tttatcccat ttaatggctt
    51541 cctgctgacc aacaacggcg cgggcaacgc ccggcacgct gagaccggca gccgccgcag
    51601 cggccgcaac ggcgttagct ttcataaagc tacgacgact gagtttcatg gtgtttcctc
    51661 accttgctct tcctgctggt gataaaccag cgacaccgcc agcacgccct ctacgttgcg
    51721 tactgactca atggtttgga tcagcgtttc gctgtcttct gcttccacca ccacaatcaa
    51781 ctgaccgctc ggcgcgtcgc tgacagcaac ttcacagccg ggaaaggcgt tcagttgggt
    51841 gctgatgtct gaaattcgtt cgcttttggc ctgcacaacc aggctgcaaa cttgccagtt
    51901 agtgtgcatg gagatactcc gcagttatgg ctgataccgg acagctggct gcacatgctc
    51961 cacatccgtt gcaaagttgg ctattaagtt gcggctggta gatcccggag agcgtcgggc
    52021 gaaagataat tgccattggc tcacagctat cctgacagcg gcggcattca actgactgat
    52081 acgccaggca cgcgtcccca atggtgaact gcaaatccca ggccctggtg tggcgcggag
    52141 aaaatagcga ttcggggcaa gcctgcgcgc aggcgtaaca gaagctgcac tcgttatttt
    52201 tgaaattaac gctcggatag ccgcccgcgc cgcgttgcag aatgttgttt tcacaagcat
    52261 taatacaggc gtcgcagcgg gtgcaatggg tcagaaaatg agattcatca ccggaccagg
    52321 gcggacggat accgttactg gctttgcgcc agcgaccagt gagtatgccc cgacgggatg
    52381 catcaatctt cacattgacc ttccatcatt aacgcgctct gaaaattgag agcgaccaaa
    52441 taaaccgcat aattaataag ccatttttat agccgctaag atattaaagg atgtgtcaaa
    52501 gatgcatacc ccgatcgagg taaaacctgt agcaggatca aaagagtggc gggaagcgtg
    52561 gcaaaaacgg gcttttgctc acat
//
LOCUS       DAZKIF010000016        47779 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZKIF010000016 DAZKIF010000000
VERSION     DAZKIF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.016"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            59..691
                     /gene="comR"
                     /locus_tag="ACHOTW_000983"
     CDS             59..691
                     /gene="comR"
                     /locus_tag="ACHOTW_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP9843141.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(753..1292)
                     /locus_tag="ACHOTW_000984"
     CDS             complement(753..1292)
                     /locus_tag="ACHOTW_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9843142.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1519..2823)
                     /gene="ndh"
                     /locus_tag="ACHOTW_000985"
     CDS             complement(1519..2823)
                     /gene="ndh"
                     /locus_tag="ACHOTW_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP9843143.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3223..3765)
                     /gene="ycfP"
                     /locus_tag="ACHOTW_000986"
     CDS             complement(3223..3765)
                     /gene="ycfP"
                     /locus_tag="ACHOTW_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP9843144.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3788..4813)
                     /gene="nagZ"
                     /locus_tag="ACHOTW_000987"
     CDS             complement(3788..4813)
                     /gene="nagZ"
                     /locus_tag="ACHOTW_000987"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP9843145.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4824..5648)
                     /gene="thiK"
                     /locus_tag="ACHOTW_000988"
     CDS             complement(4824..5648)
                     /gene="thiK"
                     /locus_tag="ACHOTW_000988"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP9843146.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5629..6270)
                     /gene="lpoB"
                     /locus_tag="ACHOTW_000989"
     CDS             complement(5629..6270)
                     /gene="lpoB"
                     /locus_tag="ACHOTW_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP9843147.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6284..6661)
                     /locus_tag="ACHOTW_000990"
     CDS             complement(6284..6661)
                     /locus_tag="ACHOTW_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP9843148.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6664..7023)
                     /gene="hinT"
                     /locus_tag="ACHOTW_000991"
     CDS             complement(6664..7023)
                     /gene="hinT"
                     /locus_tag="ACHOTW_000991"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP9843149.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7363..9552
                     /gene="fhuE"
                     /locus_tag="ACHOTW_000992"
     CDS             7363..9552
                     /gene="fhuE"
                     /locus_tag="ACHOTW_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFP9843150.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9612..11045)
                     /gene="ptsG"
                     /locus_tag="ACHOTW_000993"
     CDS             complement(9612..11045)
                     /gene="ptsG"
                     /locus_tag="ACHOTW_000993"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP9843151.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11340..12137)
                     /locus_tag="ACHOTW_000994"
     CDS             complement(11340..12137)
                     /locus_tag="ACHOTW_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9843152.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12148..13152)
                     /gene="holB"
                     /locus_tag="ACHOTW_000995"
     CDS             complement(12148..13152)
                     /gene="holB"
                     /locus_tag="ACHOTW_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9843153.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13149..13790)
                     /gene="tmk"
                     /locus_tag="ACHOTW_000996"
     CDS             complement(13149..13790)
                     /gene="tmk"
                     /locus_tag="ACHOTW_000996"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9843154.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(13780..14802)
                     /gene="yceG"
                     /locus_tag="ACHOTW_000997"
     CDS             complement(13780..14802)
                     /gene="yceG"
                     /locus_tag="ACHOTW_000997"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP9843155.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14805..15614)
                     /gene="pabC"
                     /locus_tag="ACHOTW_000998"
     CDS             complement(14805..15614)
                     /gene="pabC"
                     /locus_tag="ACHOTW_000998"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP9843156.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15734..16975)
                     /gene="fabF"
                     /locus_tag="ACHOTW_000999"
     CDS             complement(15734..16975)
                     /gene="fabF"
                     /locus_tag="ACHOTW_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9843157.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17063..17299)
                     /gene="acpP"
                     /locus_tag="ACHOTW_001000"
     CDS             complement(17063..17299)
                     /gene="acpP"
                     /locus_tag="ACHOTW_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9843158.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17510..18244)
                     /gene="fabG"
                     /locus_tag="ACHOTW_001001"
     CDS             complement(17510..18244)
                     /gene="fabG"
                     /locus_tag="ACHOTW_001001"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP9843159.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18257..19186)
                     /gene="fabD"
                     /locus_tag="ACHOTW_001002"
     CDS             complement(18257..19186)
                     /gene="fabD"
                     /locus_tag="ACHOTW_001002"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9843160.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19202..20155)
                     /gene="fabH"
                     /locus_tag="ACHOTW_001003"
     CDS             complement(19202..20155)
                     /gene="fabH"
                     /locus_tag="ACHOTW_001003"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9843161.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20223..21293)
                     /gene="plsX"
                     /locus_tag="ACHOTW_001004"
     CDS             complement(20223..21293)
                     /gene="plsX"
                     /locus_tag="ACHOTW_001004"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9843162.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21374..21547)
                     /gene="rpmF"
                     /locus_tag="ACHOTW_001005"
     CDS             complement(21374..21547)
                     /gene="rpmF"
                     /locus_tag="ACHOTW_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9843163.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21599..22120)
                     /gene="yceD"
                     /locus_tag="ACHOTW_001006"
     CDS             complement(21599..22120)
                     /gene="yceD"
                     /locus_tag="ACHOTW_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP9843164.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22319..22903
                     /gene="yceF"
                     /locus_tag="ACHOTW_001007"
     CDS             22319..22903
                     /gene="yceF"
                     /locus_tag="ACHOTW_001007"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP9843165.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23004..23963)
                     /gene="rluC"
                     /locus_tag="ACHOTW_001008"
     CDS             complement(23004..23963)
                     /gene="rluC"
                     /locus_tag="ACHOTW_001008"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP9843166.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24536..27721
                     /gene="rne"
                     /locus_tag="ACHOTW_001009"
     CDS             24536..27721
                     /gene="rne"
                     /locus_tag="ACHOTW_001009"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP9843167.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27916..28869)
                     /gene="flgL"
                     /locus_tag="ACHOTW_001010"
     CDS             complement(27916..28869)
                     /gene="flgL"
                     /locus_tag="ACHOTW_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP9843168.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLENSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28881..30524)
                     /gene="flgK"
                     /locus_tag="ACHOTW_001011"
     CDS             complement(28881..30524)
                     /gene="flgK"
                     /locus_tag="ACHOTW_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP9843169.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30590..31531)
                     /gene="flgJ"
                     /locus_tag="ACHOTW_001012"
     CDS             complement(30590..31531)
                     /gene="flgJ"
                     /locus_tag="ACHOTW_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP9843170.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31531..32628)
                     /gene="flgI"
                     /locus_tag="ACHOTW_001013"
     CDS             complement(31531..32628)
                     /gene="flgI"
                     /locus_tag="ACHOTW_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP9843171.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32640..33338)
                     /gene="flgH"
                     /locus_tag="ACHOTW_001014"
     CDS             complement(32640..33338)
                     /gene="flgH"
                     /locus_tag="ACHOTW_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP9843172.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33391..34173)
                     /gene="flgG"
                     /locus_tag="ACHOTW_001015"
     CDS             complement(33391..34173)
                     /gene="flgG"
                     /locus_tag="ACHOTW_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP9843173.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34311..35066)
                     /gene="flgF"
                     /locus_tag="ACHOTW_001016"
     CDS             complement(34311..35066)
                     /gene="flgF"
                     /locus_tag="ACHOTW_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP9843174.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35086..36291)
                     /gene="flgE"
                     /locus_tag="ACHOTW_001017"
     CDS             complement(35086..36291)
                     /gene="flgE"
                     /locus_tag="ACHOTW_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP9843175.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36316..37011)
                     /gene="flgD"
                     /locus_tag="ACHOTW_001018"
     CDS             complement(36316..37011)
                     /gene="flgD"
                     /locus_tag="ACHOTW_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP9843176.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37023..37427)
                     /gene="flgC"
                     /locus_tag="ACHOTW_001019"
     CDS             complement(37023..37427)
                     /gene="flgC"
                     /locus_tag="ACHOTW_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP9843177.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37431..37847)
                     /gene="flgB"
                     /locus_tag="ACHOTW_001020"
     CDS             complement(37431..37847)
                     /gene="flgB"
                     /locus_tag="ACHOTW_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP9843178.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38002..38661
                     /gene="flgA"
                     /locus_tag="ACHOTW_001021"
     CDS             38002..38661
                     /gene="flgA"
                     /locus_tag="ACHOTW_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP9843179.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38737..39030
                     /gene="flgM"
                     /locus_tag="ACHOTW_001022"
     CDS             38737..39030
                     /gene="flgM"
                     /locus_tag="ACHOTW_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP9843180.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39035..39451
                     /gene="flgN"
                     /locus_tag="ACHOTW_001023"
     CDS             39035..39451
                     /gene="flgN"
                     /locus_tag="ACHOTW_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP9843181.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39491..41026)
                     /gene="murJ"
                     /locus_tag="ACHOTW_001024"
     CDS             complement(39491..41026)
                     /gene="murJ"
                     /locus_tag="ACHOTW_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP9843182.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41136..42059)
                     /locus_tag="ACHOTW_001025"
     CDS             complement(41136..42059)
                     /locus_tag="ACHOTW_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9843183.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42061..42708)
                     /locus_tag="ACHOTW_001026"
     CDS             complement(42061..42708)
                     /locus_tag="ACHOTW_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP9843184.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42719..43303)
                     /gene="rimJ"
                     /locus_tag="ACHOTW_001027"
     CDS             complement(42719..43303)
                     /gene="rimJ"
                     /locus_tag="ACHOTW_001027"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9843185.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43539..44747
                     /gene="mdtH"
                     /locus_tag="ACHOTW_001028"
     CDS             43539..44747
                     /gene="mdtH"
                     /locus_tag="ACHOTW_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP9843186.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44811..45458
                     /gene="grxB"
                     /locus_tag="ACHOTW_001029"
     CDS             44811..45458
                     /gene="grxB"
                     /locus_tag="ACHOTW_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP9843187.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45592..46152
                     /locus_tag="ACHOTW_001030"
     CDS             45592..46152
                     /locus_tag="ACHOTW_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9843188.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46258..47304
                     /gene="pyrC"
                     /locus_tag="ACHOTW_001031"
     CDS             46258..47304
                     /gene="pyrC"
                     /locus_tag="ACHOTW_001031"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9843189.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47378..47623
                     /gene="dinI"
                     /locus_tag="ACHOTW_001032"
     CDS             47378..47623
                     /gene="dinI"
                     /locus_tag="ACHOTW_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP9843190.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11531 a        12906 c        11893 g        11449 t
ORIGIN      
        1 cggaggtgat tatttttata ataacctttc attcacagtc accaggtaca acggcatcat
       61 ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag
      121 ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg aagcgacttc
      181 tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg cggaatttac
      241 caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa
      301 gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg ctgactattt
      361 tgctgccatc gccaactgct ttaccggcaa agacaccccg gcaggctgct tcatgatcaa
      421 caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga aatcacgcca
      481 tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat
      541 cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta ttcaggggat
      601 gtcgatcagc gcacgcgaag gtgcatcgtt ggaaaaactg atgcagattg ccggaacgac
      661 tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc agcctgagcg
      721 aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa aatcagttgc
      781 ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc
      841 tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc
      901 gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc
      961 tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc tttaccacga
     1021 ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac cgacccggta
     1081 atgcgatgtt catcctgcac cggtcgacga tgggtcactg tgacgttgcg acactcctga
     1141 cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg
     1201 ccacgttcaa acacgttcag actggccact gccgctacgc caagcgcagc tgcgacacca
     1261 atcccgatac ccgccaacat tgatttattc acgggaatac ctccttcttt gcagttatgc
     1321 caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg
     1381 gctggaaata cttaaggatt cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc
     1441 gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt ttatgccaca
     1501 tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc ggttaatact
     1561 ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct gatgcattcg
     1621 gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg agccgcgcgt
     1681 caggttaccc atcaggctac caacggtgga gaagttcgac agcgatacca gcgaaccatg
     1741 atctttatac tgataatttt tcagcagctt accgttcatc tgcgccagaa tgttgttcat
     1801 tgcgcaagtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg
     1861 acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg tggtttgcag
     1921 cgtcagttcc accaccagct ggttgatacg gttagtttca agaccaccga tatctttcag
     1981 gaagtctggc gctttgatcc cggctgccca taccatcaga tcagcctcaa tatattcgcc
     2041 atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg tcagaacgcg
     2101 aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg
     2161 caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt
     2221 gtaaccgtag ctgtgcagtt gcttgaccgc gttgtgcaat tcagcggaga gttctacacc
     2281 cgtcgcgccg ccgccgacaa tcgcaatgtt cactttgcca tttgcgccca ggttggcgga
     2341 gtatttcagg aagagattca gcatctcctg gtgaaagcga cgcgcctggt gcgggttatc
     2401 gaggaaaatg cagttctctt tgacacctgg tgtattgaaa tcgttagagg tgctacccag
     2461 cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt cacctttctc
     2521 atcgcgcagt tctgcaatag tgattgtttt cgcgtcacga tcaatatcaa tgacggaacc
     2581 cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg catcgacgcc
     2641 ttcatcaagc gagccagtcg ctacttcgtg cagcagcggt ttccacaggt ggctgtggtt
     2701 acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc ccagctgtgt
     2761 tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca atggcgtagt
     2821 caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttatta acatagctct
     2881 taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata acatgaatgg
     2941 tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag ataggcgact
     3001 ttaccgggtg agatcaaaaa acagagttta ttgtttatta tttgagcgaa tttgtgagag
     3061 tgatatagcg tgaggggtgt atgtagggcg ttcggtaggt ctgataagag gcgcatcagg
     3121 cattgatgct cgactgtcgg atgcggcgtg aacgccttat ccgacttaca gaattcggag
     3181 cgcatcaggt accggcaagg ttacctgatg cgacgagggc atttacccga gggttttgaa
     3241 cgctttaatg cgctgcaaat gcggggagat attcttgaat ttgtgcgtct gctcttcgtc
     3301 ccagacaatc tcgtaataat gatgcaactc ttcagatgtc cgctggctgt taagcgcttc
     3361 atcattacgc gacaaaatca ccaggcaacg atcgcgattc ttctcgcgga agttggtcac
     3421 acacttagtg gcaatatcgg catactcttc cgggcgatca atcttgcctt ccatgttctc
     3481 ataagggaac aaattagggt tgaagatcac ctggcggata tcgcaaagaa aaccaatccg
     3541 ttccgcccag tatccgccca gaccaacgcc gcaaatcagg ggacgctcgt cgacgttcag
     3601 ttgcaacatt ttgtccactt ctttgagcag atgctgcata tcatgtttcg gatgccgcgt
     3661 actgtagctt atcaagcgta catccgggtc aataaactgc aattgtaaga ctttctcgtg
     3721 gttacccgga ctgttagagt caaaaccgtg taaatagata atcatcgctt cctcacataa
     3781 gccagggtta gtgacctgct ttctcttcct gccagcgttc atgtaactga ttcagacggg
     3841 cgctgatcgc tttccagcga gccgagtcca tcagttcctg tcgcgaaaat gaacctttat
     3901 gatacaaacg tgtaacacgt tctgccttga tcggtgacag attatctaac acgctgacgg
     3961 cccctttacg attattgcag accaggatca tatcgcaacc cgcatccagt gatacctgcc
     4021 cgcgttcggc ataactgccc ataatcgcgg caccttccat cgataaatcg tcagagaaaa
     4081 tcacgccgtc aaaacccagt tcctgacgca aaacggtttt cagccagtag gaagaaccgc
     4141 tcgccggacg cggatcaaca tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt
     4201 tattttcgcg gattaacgtg ctgaaaaccg acatatcttt ggcacgaatt tccgcttgcg
     4261 ggcgtgggtc gcacggcgtc tctttgtgtg aatctgcagt tactgcaccg tgtcctggga
     4321 agtgtttccc ggtcgttttc attccggctt catgcatccc atcaataaac cgactggcga
     4381 ttgccagggc tttttctggg tcggcatgat aagaacgctc gccaatcgcc gcgctgatat
     4441 gtcctacatc cagcactggc gcaaagctga tatcaatatc catagcgatc atttcgctgg
     4501 ccatcagcca acccgcctct tgcgccagtt tgccgccctc ttccattccc aacagcgcag
     4561 caaaggattg tgctgccggt aagcgggtaa aaccttcgcg aaaacgctgg acgcgtccac
     4621 cttcctgatc taccgccacc accagatgat tgcgcgatgc tgcgcggatc tggcgcacca
     4681 gttcacgcaa ctgggcagga tcatgatagt ttcgcgtaaa gagaatcagc cctcccacca
     4741 gcggatgcgc cagtatttca cgctcttccg cgtccagttc gtaaccttcg acatccaaca
     4801 ttactggacc cacactgctc tccttattgt tttattaata gctgccgcca ggtgtcatcg
     4861 gccagcctga taaattgttg atcgccggtt tgtcgccagc ggtactcaaa ccaccctgct
     4921 ttgagcatca gcagccaggg aaaccatcgc ctgacctgac gccataattg cgccggataa
     4981 atcttcgcgc gagtggcata gtcattgacc aattgccggt gctgttcagt attttccacc
     5041 cacaccgccg ccagttccag cgcgatatca ccatctcctg catactccca gtcgatgagt
     5101 tttaaccctg acgcgctatg cactaaattt ccggcgtgga catccatatg caatggactt
     5161 aagcgtaaag gccgtggttc ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc
     5221 actgtccgcc gcgccggatc gctttgctgc cagtacagtt ccagtaacgg caacagcgtt
     5281 attcgccagc caaaacgtgg ttgttgatgt agataataca gcaagcctgc cagttcgttg
     5341 gtatccggca aatacgtttt tacctcgccg ggcagatagt cgactaccat ccagtcacgg
     5401 agatataaat gcggcttcgg tgcaatgcat gcgggtagtt gtgataaagc ccgatactgg
     5461 cgcaagaacg cggactgcgg cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg
     5521 ccctgatgtt caatgagaaa actaccgcca ctaagcccac tattaaacgt cgtgacggga
     5581 tgaaactgcg ggaaaaagcg cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga
     5641 aacggcacct ttacctgacc agataatttc gcccgtctgc accagcatca gctgcatttg
     5701 tagggtcgga gcgttaacgt tgccagaggc gctggagtac agcacgtaat gagcgccgac
     5761 attgcgggca atgcctatgg ctttactacg ggtgcctaaa ctatcctgcg gcgacaaacc
     5821 taactgttgc ttagccatcg acagctgctg ggcggaaacc agggtaaatt tcccgttatt
     5881 agccagcgca tttcgcagcg tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg
     5941 gttgttaacg ctatcaacca gcaggacgct acccgcagtc accccgtcag ccccaagcat
     6001 cttactgacc atcggctgca ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg
     6061 cggtgcagtt tgatcttcgt gctcaattgg gcctggctgc tgcgggatcg tcggcaccga
     6121 gggcactgtg gggacaggct gttgtggctc ggctggttgt tccggcgctg gtttcacttc
     6181 ttctaccggt gcaggttcac gttgccccac acacccggcg agaaacatcg ccagcgcggt
     6241 aatcaaggcg tagcgactca tttttgtcat caagattcac cccttacaaa tatagataaa
     6301 gtctgacttt gtgcgccccc agaaaattgg cgctgccgta cagcgttacc gccgaatgtg
     6361 cgggaatggt aacgctgcgt ggcctttcca gaggatgcat ctccagccct ctggcgtcat
     6421 accagtaaaa acgataatga acggtaacgg gttcttgcct ttcgttataa agagttgagg
     6481 atgctgaagg ttgaatatca gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag
     6541 ccaataaaga tgactccatc accagcgatt gctcatcatt caccggaatt tccggatgtg
     6601 aacgacagcc caccagcagt aacaacgcca gagacaccag cccaaagcat ccttttctca
     6661 tcgttacaga cctttatgcg ccagcattgg tcccagcgga cggccaccca acaagtgcat
     6721 atggatgtgg taaacctctt gtccgccatg gcggttggtg ttcatgatca gacgatagcc
     6781 atcttcggca ataccttctt gctcagcaat ttttgccgct acggtgatca tgcgtcccag
     6841 cgcctgctca tgctcagctg agacgtcgtt cacagtcgga atgaggatat tcggaatgat
     6901 cagaatatgc gttggcgctt gcggcgaaat atcgcgaaac gccgttacca gatcatcctg
     6961 gtagacgata tcggagggga tctcacgacg aataattttg ctgaatatag tttcttctgc
     7021 cacgacattt tcctttttca aatagccatg cgttgcgcca tgctacgccg cgccacactg
     7081 cgagtataga gtatggtcga gttaaacctg ctctttcaac cttaactcac gatttcttaa
     7141 gcaaaaaatc gttttgctgc cgtatatatc tccattattc ccatttctgc ctgcgaatgc
     7201 gctgaaaaga cattctctgt ttggtttaca cgacaaatat gaatgcgtat atttctcatt
     7261 tgcatttgca tttgcaagca aaattattcg cacactaaat ataaccgctc atcaccctcc
     7321 tccggcatga gcctgacgac aacataaacc aagagatttc aaatgctttc aacacaattt
     7381 aacagggata atcaatatca agccatcacc aaaccgtcac tacttgccgg ttgcatagca
     7441 ctggcactat taccttctgc cgcttttgct gcaccagcca ctgaagaaac ggtgattgtt
     7501 gagggttcag ccacagctcc agatgatggc gaaaatgatt acagcgtaac gtctacctct
     7561 gcgggtacca aaatgcagat gactcaacgt gatattcctc agtcggtcac tattgttagc
     7621 cagcagcgga tggaagatca gcagttacaa acgctgggcg aagtgatgga aaacacgctg
     7681 gggatcagca aaagtcaggc ggattccgat cgtgctcttt attattcccg cggattccag
     7741 atcgataact atatggttga tggtatcccc acctattttg aatcgcgctg gaatctgggc
     7801 gacgcacttt ctgatatggc actgtttgaa cgcgtagaag tagtgcgtgg cgcgacagga
     7861 ctcatgaccg ggacgggtaa tccatctgcg gcaattaata tggttcgaaa acacgcgacc
     7921 agtcgtgaat ttaaaggcga tgtctcggcg gaatacggta gctggaacaa agaacggtat
     7981 gtggcggatt tacaaagccc actcaccgaa gacggtaaaa tccgcgcgcg aattgtcggc
     8041 ggctaccaga ataacgactc atggctggac cgctacaata gtgaaaagac cttcttctcc
     8101 ggcattgtcg atgctgattt aggcgatctt acgacgcttt cagccggtta cgaatatcag
     8161 cgcattgatg ttaatagccc aacctggggc ggtttaccgc gctggaatac tgatggcagc
     8221 agcaacagtt acgatcgcgc acgcagtacc gcacctgact gggcgtacaa cgataaagag
     8281 atcaacaagg tctttatgac cctgaagcag cggtttgctg atacctggca agcgacactg
     8341 aatgccaccc actctgaagt cgaatttgac agcaaaatga tgtatgtcga tgcctatgta
     8401 aacaaagcgg atggtatgct ggttgggcca tacagtaatt atggacctgg ctttgattat
     8461 gtcggcggca ccggttggaa cagtggcaaa cgtaaagttg atgcgctgga tttgttcgct
     8521 gacggtagtt atgaattgtt tggtcgtcag cacaatctaa tgtttggtgg cagttacagc
     8581 aaacaaaaca atcgttactt cagttcatgg gccaacatct tcccggatga aattggcagt
     8641 ttctacaact ttaatggcaa tttcccacaa accgactggt caccacagag cctggcgcag
     8701 gacgatacca cacatatgaa atcgttatat gctgccactc gtgtcaccct tgccgatccg
     8761 ctgcatctga tcctcggcgc acgttatacc aactggcggg ttgatacgct gacttacagc
     8821 atggagaaaa accacaccac gccttacgct ggtctggtgt ttgacatcaa tgacaactgg
     8881 tcgacctacg ccagctatac ctctattttc cagccgcaaa atgatcgtga cagttcaggc
     8941 aaatatctgg ctccaatcac cggtaacaac tacgagctgg gtctgaaatc ggactggatg
     9001 aatagccgtc tgaccaccac gttagccatc ttccgtattg agcaggataa tgtcgctcag
     9061 tccaccggta cacctatccc cggcagcaac ggcgaaaccg cctataaagc ggtggatggg
     9121 acagtcagta aaggggtgga atttgaactc aacggcgcaa ttaccgacaa ctggcagctg
     9181 acatttggcg caacgcgcta tattgcagag gataacgaag gaaacgccgt taatcctaat
     9241 ctgccacgca ccacggttaa aatgttcacc agctatcggt tgcctgtcat gccagagttg
     9301 acagtcggcg gtggtgttaa ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat
     9361 ggcaccttcc gcgccgagca aggtagctac gcgttggtgg atctcttcac ccgctaccag
     9421 gtgacgaaaa acttctcgtt acaggggaac gtcaataacc tgttcgacaa aacctacgat
     9481 accaacgtgg aaggttctat cgtctacggc gcaccgcgta atttcagcat taccggcacg
     9541 tatcaattct gaataacacc tgtaaaaaag gcagccatct ggctgcctta gtctccccaa
     9601 cgtcttacgg attagtggtt acggatgtac tcatccatct cggttttcag gttatcggat
     9661 ttagtaccga aaatcgcctg aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt
     9721 ttcttcaggc cagcctgatc cactttagac acatcagcaa cgctgacgcg cagacgggta
     9781 atacatgcgt cgaggttagt aatgttttct ttaccaccaa atgcagcaac cagagccggt
     9841 gccatttcgc tggtacctgt cgcttttgca tcttcagtcg cgtcttcacg acccggcgtt
     9901 ttcagatcca gtgctttaat cagcacgcgg aagatggtgt agtaaacaat cgcataaccg
     9961 ataccgacga tcgggaacag ccacagtttg ctgctgttac cagacagaac gatgaagtcg
    10021 atcagaccgt gcgaaaacga cgtaccgtca cgcatcccca gaagaataca gattgggaat
    10081 gccaggcctg ccagaatcgc gtggatgatg tacaggatcg gcgcaacgaa catgaaggag
    10141 aactcgatcg gctcggtgat accggtcagg aacgaggtca gcgccgcgga gatcataata
    10201 ccgcccactt tcgcgcggtt ttctggttta gcagagtgcc agatagcaat tgcggcagct
    10261 ggcagaccgt acattttgaa caggaagcca ccagacagtt tacccgcagt cgggtcaccc
    10321 gccatataac gcggaatgtc gccgtggaaa acctgacctg ctgcgttggt gtattcacca
    10381 atctgcatct ggaaaggtac gttccagatg tggtgcagac caaacggtac caggcaacgt
    10441 tcgatgaaac cgtaaatgcc aaacgcaact accgggttct ggtaagcagc ccactgagag
    10501 aaggtctgga ttgcagaacc aatcggcggc caaatgaagg acagcacaac gccagtaaag
    10561 atggcagcca ggccagaaat gatcggcaca aagcgtttac cggcaaagaa gccaagatac
    10621 tcaggcagct taatacggta gaaacggtta aacatgtacg ctgcgatcgc accggagata
    10681 atccctccga gtacgccagt atccgccagg tgtttagagg cgatttcttc agcaggtaaa
    10741 tgcagtacca gtggcgcaac cacggccatg gttttaacca tgatgccata ggcaacaact
    10801 gcagccagcg cggatacgcc atcgttattg gtaaagccga gggcgacacc gatcgcaaaa
    10861 atcagtggca tgtttgcaaa gacggaaccg cctgcttctg ccataacatg cgatacaacg
    10921 gcgggcagcc agctgaaatt cgcggaaccg acgcccagca gaatacctgc gataggcagt
    10981 acggataccg gcagcatcag cgatttaccg accttttgca ggttagcaaa tgcattctta
    11041 aacataattg agagtgctcc tgagtatggg tgcttttttt acgttctcac gcgtggcaag
    11101 gggggagagc ctcgccgtgt acagggcatc taagcgccct ttatttatta cacagagtaa
    11161 aataattcag tgccaatttg tttgacggct atcacgtttc aacttcagat cattggagaa
    11221 gttgcacatt ttgacaaaac atatgtcaca atatttcaaa cgtggttatt caccgcccaa
    11281 ttttcggcgg tgaactttac tcgttttagt cattaattac gagctttaaa aaaactcatt
    11341 caacggatgg attgaaggcg ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg
    11401 gttacctgcg ccagttcttc aacggcaaca cctttcaaca cagccatgta ttctgcaacg
    11461 tcacgaacca tcgcaggttg attctctttt cctcgatgcg gcaccggcgc aaggtaaggt
    11521 gagtcagttt ccaccagtaa ccgatccagg gggacataac gcgcagcatc gcgcagttgc
    11581 tccgcattac ggaaggtcac aatgccggaa aaggagatgt aaaatccgag atccagtaat
    11641 ttgcccgccg tttctctgtc ctctgtaaaa cagtgtagta cgccaccgca atccgtcact
    11701 ttttcttcgc gcagaattgc cagcgtatcg gcgcgggcgt cacgggtatg gacgataacc
    11761 ggcttgttca gttcacgacc aatctggata tgatggatga aggactcttg ctgacgtact
    11821 ttagtttccg gtgtgtaata ataatccagc ccggtttcac ccagcgctac aacaccctct
    11881 tctgccgcca gacggcgtaa atcttctaca tcgtagggat cattctggtt gagtggatgc
    11941 acgccacaag aaaatacgac gttgtcacgt tcgcctacca gatcccgcat gtgtaaataa
    12001 cccggtaatg ttgtggcgac tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc
    12061 agaacgtcat ccacgtcctt atgcaaagat tcataatcca gaccatcgag atggcagtgt
    12121 gagtcgacta aaaacatgat gtctctctta aagatgagga accggtagca caacgcccgg
    12181 ttgcaggtaa tgctcaatac gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc
    12241 tgtgacagac attaactgtt cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg
    12301 cgagggagaa agatggtttg ccagttcggc gaccaggccc ggcacatcaa cattggtcac
    12361 ctgcgcagcg ccatgatggc gttttagcgc atccatcagc aacgttgcca gccagtgtaa
    12421 acgcgccgga gcttgttcat gattaagggc cgccagcagc gaataccagt cgcccgatgg
    12481 cacgctatat gccaacgcct gacacaatgt ttcacgagcc tgccagttat ctccctgaaa
    12541 caacgccagt gccgcgccag gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc
    12601 ctgtgacatt gtcacttcgc gtgaaagcca ggtcacggcg tactgttccg gcggcggcgc
    12661 aaggtaatgt aaccgacaac gactacgtaa cgttgccagt aaacgttcag gctcgcgggt
    12721 agccaggaaa aaccaggttt ctgctggtgg ctcttcaagc gttttcagca atgcgttagc
    12781 cgcggcgtcg gtcagtaagg cagcatcggt tacccagaca actttcgcac cacctaagcg
    12841 tgcgtgctca ttcagctttt cggtgacctc acgtaccgca tcaatgccca gcgtattttt
    12901 ccctttttcg ggagccaggg tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca
    12961 tccacgacag tgaccgcaac ttttgtggcc ctgcggttgt tggcagagta aataacggct
    13021 cagggcgtag attaaagcat catcgcccat gcccggtaac gcctgaatga gtagcgcatg
    13081 gtgacctctt ccggcctgat agctggctac cagtttttcg aaatcaggtc gtaaccatgg
    13141 ataccatctc atgcgtccaa ctccttcacc cagcgggtca cggtagtgcg gattgcatcc
    13201 atcacggcct ccagcggctg ggtggcatca atggtatgaa tacttttatc ttgtgcagcc
    13261 agttccagat agcgggcgcg ggtgcgatta aagaaatcga aagactcttg ctcaatacga
    13321 tccagctcgc cgcgcgcacg cgcgcgtttt aagccaactt ccggggtaac atcgagatag
    13381 agcgttaagt cggggcgaaa atccccgaga acagcatcac gcagtgttgc cagcatatgt
    13441 tggtcaatac cacgtcctcc gccctgatac gcctgagtgg agagatcgtg gcgatcgcca
    13501 ataacccagg tgccgttagc cagcgcaggt ttgatgaccg tttctaccag ttgaacgcgc
    13561 gcggcataaa acatcagaac ttcggcttta tcggtaatga tttcatcgcc taccgatttg
    13621 atatccagca ccaggcttct taacttttcg gcaagttgcg taccaccagg ttcccgagtg
    13681 aaaaccatgt cgcggatacc cagttgctcg agcgtctcaa ccaccacatt acgcgcggta
    13741 gttttgcctg cgccttccag cccctcaatg acgatatact tactgcgcat ttttttcctt
    13801 aagcactttc agataatcct gcacagactt gttatgactg gcaagattgg tattaaacgt
    13861 gtgaccacct ttaccatcgg ccacaaaata gagatacggc gtttttgccg gatgcgcagc
    13921 agccttcagc gaatccgccc ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt
    13981 ataggtgtta tacgctgtcg gcgtttccag gtctgcacga gaaagtttgc cattataacg
    14041 ctctcccatc ccgtaaatca cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg
    14101 gttgataaat accgaggcaa cctgatcgcg ttcactggca atggcggttt ctttttcgat
    14161 aattgatgcc atcgtcacca gctgattttt atctttataa ggcagaccgt ccgcacgccc
    14221 ttcccaggcg ctatcgaccg ctttcaccat tttcttgtgc gctcgcttga gtaacgcgac
    14281 atcggtggta ttggcggtat acatccaggt gtctggccag aaccaacctt caatccactc
    14341 cgggttttcc agttcaagtg cctgcgctac ggtgatgtac ttatcatcgc tcagcgtgtg
    14401 cttgatatac ggggcctcac gcaattgctt gaggtagtcg ctcagacgca tcccttctac
    14461 cagtcgcaga gggaactgtg cttctttacc gctttccaac aatttcagca tctcgcgcac
    14521 ggtcatctgc ggtgtaaagc ggtaagtccc ggctttaaag tgagaaagat ccggttcgat
    14581 acgcagcaac cattgaaaaa cccgtgggcg attgatgatc ttatcggcat aaagctgttc
    14641 accaagcgcc agacgtccgg tccctggctt cagggtaaat atcgtctctt ctttgataag
    14701 caatttgctg tcggcaagat agcgaacctt ccagacgccc acaccagcgg cgatacccag
    14761 taccaccagc aataacaaga ttatcaataa cacttttttc atgactaatt cgggcgctca
    14821 caaagtgggg ctaaatattc atataacgtt gctgacgaaa aggagacatc gccacaggca
    14881 cgtacgggca tcactggcat taacgcatta caaataacca tctcatctgc ctgcaacgcc
    14941 tcttccagag aggcttgcac ttcgacaagc tgataagagg attgtgccag caaacggata
    15001 cagaattgtc gcataatgcc gttaacacct gcctgatcca gtcgcggcgt ataaactacg
    15061 ttgcccttcc gccagaacaa attagctgca cagcattccg taaccccccc ttcgctgtca
    15121 aggaccagcg cctcatcagc gtttgtctgc tcaagatgag agcggatcaa tacttgctca
    15181 agccgattaa gatgtttaat acctgcaaga tgaggattgc gccccagccg caccgggctt
    15241 agcgccaacg tgatcccttc gttacgcaaa cggtcgtaat gtgcaggata agccgtaacg
    15301 gagagaatcc gcgttgccgg tccactgttc aatgtgctgt accctcgccc gccactaccg
    15361 cgactgatca cgactttcag tacaccattt tgctgttctg ctgccagcgt tttcatctct
    15421 tgttcaagct gaggccagaa gtcacaggaa atcatcaacc gctgacaagc atcctgtagt
    15481 cgctggatat gcgccgataa caaactgact ttaccgtcga taactctggc ggtggtaaaa
    15541 caaccatcac caaactgcgt tgcccgatcg cttactgaca gcgattcctg cttataaccg
    15601 ttaattaaga acataccggc tccttatcag agaattgtta gtgtggcagc atgttcacta
    15661 cggaacaagt cggaataaaa gctaagaaaa aaggcccgca agcggacctt ttatatgggt
    15721 ggaaaatgac aacttagatc tttttaaaga tcaaagaacc attagtgcca ccgaagccga
    15781 aggagttaca cagcgtgtat tccattccgc tgacctgacg tgcttcatgc ggtacgaaat
    15841 ccagatcgca accttcatcc gggttatcca ggttgatggt tggcggaaca gcctgatcgc
    15901 gcagcgccag gatggagtag atagattcta ctgcacccgc cgcacctaac aggtgaccgg
    15961 tcatagattt cgtggagctt accaacacac ggcttgcagc ttcaccgaag atggttttca
    16021 ccgcctgcgc ttcagcttta tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc
    16081 caatctgact cgcttcaatg cctgcatcac gcagagcatt tgccattgcc agagctgcgc
    16141 ctgcgccatt ttctggcggt gacgtcatat gataagcatc gctgctcata ccaaagccga
    16201 cgagttcagc gtaaattttc gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca
    16261 gcataccggc accatcgccc agtacgaaac catcacgctc tttatcccac gggcggctcg
    16321 ccgcttgcgg gttatcattg cgggtagata atgcacgtgc cgcgccaaaa ccaccaacac
    16381 ccagcggcgt actggctttc tctgcgccac ctgcaaccat cacgtcagca tcgccatacg
    16441 cgataatacg cgcagcatgg ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag
    16501 agatgcttgg gccacgcagg ccatacatga tagtcagatg acctgccacc atgttcacaa
    16561 tcgttgacgg aacgaagaat gggctgatct tacgtggacc accgttcatc agagatgtgt
    16621 ggttttcttc gatcagtccg aggccgccaa tcccggagcc aattgcggca ccaatgcggg
    16681 ttgcgttctc ttccgttatt tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa
    16741 ttccatattg aatgaaggca tccatcttgc gctgttcttt gcgcgagata atgtcctcac
    16801 agttaaaatc ctttactaag ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt
    16861 cgattaggct gatgccactc tgaccggcaa gcagagcttt ccaggtagac tctacggtat
    16921 tgccgacagg agacaacatg cccagtccgg tcacaactac acgacgctta gacacgtttg
    16981 tcctccaggg agggaaaaaa tgattctagt gggacaaaaa gataaaactc aggcggtcga
    17041 acgaccgcct ggagatgttc acttacgcct ggtggccgtt gatgtaatca atggcagcct
    17101 gaacggtggt gattttctca gcttcttcgt ccggaatctc agtatcaaac tcttcttcca
    17161 gagccattac cagctcaacg gtgtcaagag aatccgcgcc taggtcttca acgaaagaag
    17221 cattgttggt aacttcttcc tgcttaacgc ccagctgttc gccgataatt ttcttaacgc
    17281 gttcttcgat agtgctcata ctcttaaatt tcctatcaaa actcgctttc gcgatggttt
    17341 tcgtagtgta taaaatgttg aaaaatttgc aactaaatcc cggcaggtct taccacgatt
    17401 ttacgttatt ttgaggcctt accccccaat aacgcaaatc attttgcact aattattact
    17461 cataaccacg cagactgcgc gcaacatgag cattttgtgc aaatcgcggt cagaccatgt
    17521 acatcccgcc gttcacatgc aaagtttcac ccgtgatgta agctgcttcg tcggatgcca
    17581 ggaatgcaac cgcgttggcg atttcctgtg cgccgccgag gcgacccgca ggaacctgca
    17641 ccaggatacc cgcacgctgg tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc
    17701 ccggagcaac aacgtttaca gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac
    17761 tgaagccgat caagcccgct ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg
    17821 taccaaccac agaaccgata gtgataatac gaccatgacg ctttttcatc atagcgcgca
    17881 ttaccgcttt tgacagacgg aaaacagatg aaaggttggt ttcgataata tcgttccact
    17941 cttcatcttt cattcgcatt aacaggttat cacgagtgat accggcatta ttgaccagga
    18001 tatccacttc accaaattct gcgcgaattt tttccagaac agattcgata gatgccgggt
    18061 cggtcacatt caacatcaga cctttgccgt tagcacctaa ataatcactg atcgcctgag
    18121 cgccattttc actggtcgca gtgccaataa ctttcgcgcc acgggctgcg agcgtttcag
    18181 caattgcgcg gccaattccg cggcttgcac cggttaccag tgcgattttt ccttcaaaat
    18241 tcatgatgtt cctcttttaa agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg
    18301 ccgaggcggt cagggtgtcg acaatgcgtt tcgtcaggcc agtaagcact ttgcccgggc
    18361 cgacttcata gagatgttct acgccttgcg ctgccatgta ctcaacagac ttcgtccact
    18421 gaaccgggtt atacaactga cgtaccagtg cgtcacgaat ggcatcacca ttggtttcgc
    18481 atttcacatc aacgttattc acaacaggaa ctgttggtgc gttaaaggtg attttcgcta
    18541 attctactgc cagtttgtcg gctgctggtt tcatcagcgc acagtgagac ggtacgctca
    18601 ctggtaacgg cagagcgcgt tttgcgcccg cagctttaca ggcagcgcca gcacgctcaa
    18661 ccgcttcttt atgaccggca ataaccacct gtcccggaga gttaaagttt accggagaaa
    18721 cgacctgacc ttctgcagct tcttcacacg cttttccaat agacgcatca tccagaccga
    18781 tgattgccgc catagcgccc gttccttccg gtacggcttc ttgcatgaac ttgccgcgca
    18841 tctcaaccag acgcaccgca tcagcgaaat caatcacacc agcgcaaacc agcgcggagt
    18901 attcccccag gctgtgaccg gccatcattg ccggtgcttt accgccatgc tgctgccata
    18961 cgcggtacag cgcaacagag gcagtcaaca gcgctggctg ggtttgccag gttttattca
    19021 gttcttcagc tggcccctgc tgggtcagcg cccacaggtc gtagcccagc gccgcagaag
    19081 cttcagcaaa cgtttcttcg acaattggat agctcgccgc catatcagcc agcattccaa
    19141 cggtttgaga accctgtcca gggaacacaa atgcaaattg cgtcatgttt taatccttat
    19201 cctagaaacg aaccagcgcg gagccccagg tgaatccacc gccaaaggct tcaagcagaa
    19261 ccaactgccc cggcttgatg cgcccgtcgc gtacagcttc atccagcgcg cacgggacag
    19321 aggccgcaga ggtattaccg tggcgatcca gcgtcaccac gacattgtcc atagacatac
    19381 cgagtttttt cgccgttgca ctgataatac gcaggttagc ctgatgcgga accagccagt
    19441 ccagttgaga acggtcaaga ttattcgccg ccagcgtctc atcaacgatg tgcgccagtt
    19501 ccgttaccgc aaccttgaag acttcgttgc ccgccatcgt cagatgaatt gaattctctg
    19561 gattcacgcg gtcggcgttt ggcagcgtca gcaattcacc ataactaccg tcggcatgca
    19621 gatgggtgga aatgattccc ggctcttcag aggcagccag caccgcagcg cccgcgccat
    19681 cgccaaaaat aataatagtc ccacgatctg ttggatcgca ggtgcgcgcc agtacatcgg
    19741 aaccgacgac cagagcatac ttcaccgccc cagatttcac gtattgatcg gctacgctta
    19801 atgcataggt gaaacctgcg caggctgctg caacgtcaaa tgccgggcaa cctttaatgc
    19861 ccaacatgct ttgaatctga caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg
    19921 ccacaacgat caggccaatc tggtctttct caatgcccgc catctcaatt gcgcgtgtcg
    19981 ccgcttcaaa gcccatggtt gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga
    20041 taccggtacg agtgacaatc cactcgtcag aggtgtccac cattttttcc aaatcggcgt
    20101 ttgtccgcac ttgttcgggc agatagctgc cagtaccaat aatcttcgta tacatgtacg
    20161 ctcagtcact tttcggttat ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc
    20221 tgctaccgca gagttccgct tttgccaccg tccagcagct caaaaccagc tgggtataca
    20281 gattccaggc gagcggcaat tcgctgagga acttgtcgct gcaccgcctg cactgcctgt
    20341 tcaatcgcga ccgcaaaagc tcgctgattg gctgcaccat gactttttat caccgtgccg
    20401 cgcaatccta acagacaggc gccgttatac tggtcggggt tgaggtgact gaatcgcctc
    20461 gtcaggctct tttgtagcca acgctttaat aacagtagcc accacgaccg ttttttccct
    20521 tcaccctgag atttcagcag agaaaggaac atcctgacaa caccttccat cgtctttaat
    20581 gtgacatttc ctgtaaagcc gtcacaaacc agcacatctg tcttgccagt taacaactca
    20641 ttggcttcaa gatagccgat ataattgata gaagggattg ttttaagcac cgctgaggca
    20701 tcccgaatac tatcgagacc ctttacttct tcttcaccaa tattgagcaa cgccacgcga
    20761 ggattgggaa tttccaccac ctcttcagcc aggactgagc ccataatggc aaattgcacc
    20821 aacattgtgc tgtcacaatc gacgttggcc cctaagtcaa ggaccaccgt tttgcccttt
    20881 tgctgatgtg gtaataccgt caccagcgcc ggacgctcaa tcccctccag gggcttgagt
    20941 aataattttg ccagccccat cagtgccccg gtattaccgg cactgacaca ggcttgcgct
    21001 cgaccttctt tcaccagctc cagggccacg cgcattgaac tcccacgact ggcgcggata
    21061 gcttgcgaag gccgggcatc actggcgata actgactgcg caggaataat ctgcagacgc
    21121 gaacgttgtt caaagtcagc tttagcaagt aatggcgtga tggcgtcggg attgccgact
    21181 aaaagaagag tgagttgcga attagaattc agtgcctgca atgctgcagg cactgtcacg
    21241 gaagggccaa aatcccctcc catgacatct aacgccaggg ttagacgtgt caaggtatcg
    21301 tcgccgcccg gtttggtctt tccccagttg cctggggaaa atcctcacta agcttcatca
    21361 cgcagacgcg tgattactta gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga
    21421 tgtggtgacg caggtgtttt tcaccagaag ttttgtctac agacaggctg gtgactgcgg
    21481 tcagcgcgtc atgggaacga cgcatgccac gtttggaacg ggttggttta ttctgttgta
    21541 cggccatgga ccttactcct caattacggt ttatccccga tccaacgggg agcaccaatt
    21601 acttacgctt taagctggct aatacggcaa atgggtttgg cttttgcgct tcttcaggca
    21661 gttcaccaaa gaccatgtcc gcttcggaca cttcacagtg ttcagaatca tgcaccggaa
    21721 ctaccggcaa ggcgaggatg atttcatctt caaccattgc aagcagatcg atttcaccga
    21781 attcgttaac ctcaatcggt tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac
    21841 gcacaggact aaaacaatac gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct
    21901 gacactcgag cgttaccgtc accttcgcat cgccgtttaa cactgcgaga cgttggttat
    21961 cgatagcgaa cgacatggag cattccacat cactgtccac actgactacg gattcggcga
    22021 cgcgctcaac ctgatcagga gtatagatac cctggtaatc aaggcgtttt tgagccgtac
    22081 gaaccggatc gagagtcagg ggtaatttta ccttttgcat agggcgcgca tattaacttt
    22141 gtaacgtcat agagtcaaag aaaaaggcag cctgtggttg ccttttgcca ataattcgca
    22201 cacattgcgg gttactgctt tattttcgtt cagtgctgat aacgaacatc aacctgcaaa
    22261 acgaagcgca tagtttaaaa tggctatcat caatacgcta tatctgatgg aaaaaaatat
    22321 gcctaaactt attttagcct ccacctcgcc ctggcgtcgc gctcttctgg aaaaactgca
    22381 aatctctttt gaatgtgcag cacccgaggt cgacgaaact ccacacagcg acgaatcacc
    22441 gcgacagttg gtgcttcgac tggcacaaga aaaagcgcaa tctctggcgt cacgttatcc
    22501 ggatcattta attattggtt cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa
    22561 accgttaacg gaagaaaatg cccgtctgca attacgcaaa gccagcggca atatcgtcac
    22621 tttctatact ggactggcgt tgtttaattc ggcgaatggg catctgcaaa cagaagtgga
    22681 gccttttgac gtccatttcc gtcacctgag cgaggcggag attgataatt acgtgcgtaa
    22741 agagcatccc ctgcactgcg cgggtagctt taagagtgaa ggatttggca ttactctgtt
    22801 tgagcgttta gaggggcgtg atcctaacac gctggttggt ttaccgctaa tcgcgctgtg
    22861 tcagatgtta cgtcgggaag ggaaaaaccc gctgatggga tagttttttg cgccggatgc
    22921 ggttacgtcg catccggcaa tcggtgctgg cggcctgatg cggcgtaaac gccttatccc
    22981 attatcatgc ttatatgctc gtattagcgc gcgttacgca gcttttgcaa acaacgcttc
    23041 aaaccatcat ccatcggcgc ttcgatacgc atcacctcac cggtccccgg atgggtaaac
    23101 ttcaacgctg cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt
    23161 ctgtcaaatt cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc tgcatactgt
    23221 gtatgcacac ggatctgatg agtgcgccct gttaccggac tacaacgcac cagggtggca
    23281 aatgcatagc gttcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactcaca
    23341 cgcacgatac gttcgccgct ttgcagaata tttttcaata acggcgcctg aacgctcttc
    23401 acatgcgact gccactgacc gcgcaccagc gccaggtaat ctttttgcat ccctttttca
    23461 cgtaattgct catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct
    23521 gaggtgtccc ggtcaagacg atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc
    23581 cgcaaacctt caataacgcc gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa
    23641 ggtttattca gcaccaggat gtgatcatct tcatataaga tgacgtccgc cagcgccgcc
    23701 accttttgca gatgtggcga aaccgcctct tcttcccgct cagcaacgcg aaccggtgga
    23761 atacgcacct catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc
    23821 cgcacttcgc ctttacgcaa aatacggtaa atcatacttt ttggtacgcc tttcaattgg
    23881 gtacgcaaaa agttgtcgat acgttgcccc gcttcgtcgg cggtgatagc aacaattttt
    23941 acggatggag tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca
    24001 ctcatttttc tatgcttata tttagttttg caccttatta cttcactacg tgatcacttt
    24061 attgatggtt attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga
    24121 taaatggtaa aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc
    24181 gtgtccatcc ttgttaaaac aagaaatttt acggaataac ccattttgcc cgaccgatca
    24241 tccacgcagc aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg
    24301 cagtccttac cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat
    24361 aattgcgctg tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg
    24421 acgataaccg tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa
    24481 ggcgttactt tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa
    24541 aagaatgtta atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca
    24601 gcgtctgtat gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta
    24661 caaaggtaaa atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc
    24721 tgaacgtcac ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta
    24781 cagtgctcat ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt
    24841 tcagatcgat aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct
    24901 ggcgggtagc tatctggttc tgatgccgaa caacccgcgc gcgggtggca tttctcgccg
    24961 tatcgaaggc gacgaccgta ccgaattaaa agaagcactg gcaagccttg aactgccgga
    25021 aggcatgggg cttatcgtgc gcaccgctgg cgtcggcaaa tctgctgagg cgctgcaatg
    25081 ggatttaagc ttccgtctga aacactggga agccatcaaa aaagccgctg aaagccgccc
    25141 ggccccgttc ctgattcatc aggagagcaa cgtaatcgtt cgcgcattcc gcgattactt
    25201 acgtcaggac atcggcgaaa tccttatcga taacccgaaa gtgctcgaac tggcacgtca
    25261 gcatatcgct gcattaggtc gcccggattt cagcagcaaa atcaaactgt acaccggcga
    25321 gatcccgctg ttcagccact accagatcga gtcgcagatc gagtccgcct tccagcgtga
    25381 agtccgtctg ccatccggtg gttcgattgt tatcgacagc accgaagcgt taacggccat
    25441 cgacatcaac tccgcacgcg cgacccgcgg cggcgatatc gaagaaaccg cgtttaacac
    25501 taacctcgaa gctgccgatg agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct
    25561 gattgttatc gacttcatcg acatgacgcc agtacgccac cagcgtgcgg tagaaaaccg
    25621 tctgcgtgaa gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata tttctcgctt
    25681 tggcctgctg gaaatgtccc gccagcgcct gagcccatca ctgggtgaat ccagtcatca
    25741 cgtttgtccg cgttgttctg gtactggcac cgtgcgtgac aacgaatcgc tgtcgctctc
    25801 tattctgcgt ctgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat
    25861 tgttcctgtg ccaatcgctt cttacctgct gaatgaaaaa cgttctgcgg taaatgccat
    25921 tgaaactcgt caggacggtg tgcgctgtgt aattgtgcca aacgatcaga tggaaacccc
    25981 gcactaccac gtgctgcgcg tgcgtaaagg ggaagaaacc ccaaccttaa gctacatgct
    26041 gccgaagctg catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa
    26101 gcgtccggaa caacctgcgc tggcaacctt tgccatgccg gatgtgccgc cagcgccaac
    26161 gccagctgaa cctgccgcgc ctgttgtagc cccagcaccg aaagctgcac cggcaacacc
    26221 agcaactcct gcacaacctg ggctgttgag ccgcttcttc ggcgcactga aagcgctgtt
    26281 cagcggtggt gaagaaacca aaccgaccga gcaaccagca ccgaaagcag aagcgaaacc
    26341 ggaacgtcaa caggatcgtc gcaagcctcg tcagaacaac cgccgtgacc gtaatgagcg
    26401 ccgcgacacc cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa
    26461 tcgtcgccaa gcacagcagc agactgccga gacgcgtgag ggccgtcagc aggctgaggt
    26521 aacggaaaaa gcgcgtaccg ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg
    26581 ccgtaacgat gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca
    26641 atctgttcag gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca
    26701 gcgtcagctc aatcagaaag tgcgttacga gcaaagcgta gccgaagaaa cggtagtcgc
    26761 accggtagct gaagaaactg tcgctgccga accaattgtt caggaagcgc cagctccacg
    26821 cacagaactg gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga
    26881 agagaacaat gctgataacc gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc
    26941 tcgtcacctg cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac
    27001 ccaatcgcca atgccgttga ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt
    27061 ctggatccgc tatccgattg tacgtccaca agatgtacag gttgaagagc agcgcgaaca
    27121 ggaagaagtg caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc
    27181 ggttgttagc gcgccagttg ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt
    27241 tgccgaaccg caaccggaag tggttgaaac gacgcatcct gaagtgattg ccgccgcggt
    27301 aactgaacag ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagttgcaga
    27361 acacgcagaa ccggtggttg aaccgcagga agagacggca gatattgaag aagttgccga
    27421 aactgctgag gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt
    27481 cgctgaagtc gcagcagaag ttgaaacggt aaccgcggtt aaaccagaga tcaccgttga
    27541 gcataaccac gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc
    27601 accgcgtcac agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg
    27661 tggtcatacg gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta
    27721 ataattagct caagtaatca agccctggta actgccaggg cttttttatt tcatctttga
    27781 atcaacatat taaaattcat cgaatggcat ccttgctaac caacaatgca aaataggcaa
    27841 aaaaatgccc gccccggcga tccctgagcg ggcagacaaa acatacccag tttcatgata
    27901 tgttttaaag cgagcttatt tgttgagctg gaagagcgac aatccctgca tatcggtaaa
    27961 tgctttatac gatgcctgca atgccgtttg ctgcatgatg taagatgaaa tagttgcatt
    28021 ccagtccaca tcaaccagat cgctcatctg ctgcgtttgc cctaatgcgc gatcgctacc
    28081 taatgaatcc agcgactcca gttcgttcag ctgcgtgcct aattccgcgc gaacggtcag
    28141 gacattgttc agcgagtttt ccagtccgcg gttggtttta tctaacgccg cagcggcggt
    28201 ttctttatcc gcttcgctat ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag
    28261 catggcaaaa agattggttt cagaagcgct accgtctggt tccgctaccg cgttactggt
    28321 aatactgtcg aaaattttgt cgcccgtatg tcctatcacc atcgaacgcg aagcatcgac
    28381 ctgttgctta atactttctg ttccgccgac ataatcaccg ttaacctcac taaacggcgc
    28441 agtctctgtt ttataaccgg caaaaatgta gcgcccgtta ccgtcagtgg tgttcgccag
    28501 attcagcaac tggtcacgaa gcccctgaat atccgtagcc agcgaggccc ggtcattgtc
    28561 actcaaggtg ccattgctgg cgtagacaat tttttcctga gcattctgga tagcggtggt
    28621 gacctggcta agtacactct cttccagtga cactttttga gtggcgaaag tacgtgccag
    28681 cgtgtactgg ctgttttgcg cctgtgcctg ggagagaaca acagcttgtg atgcagcaat
    28741 gggatcgtct gaagggttaa cgactcgctt acccgtcgac atctgctcgc cgtacttcat
    28801 ccattctgcc tgagaattgg tgatgccgcg catgttttgc tggtacatca tctgggtact
    28861 gaaacgcatt ttatctcccc ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc
    28921 gtctgcagaa cctgcgcatt cgccaggtaa tactgctgaa aacgttgcag atttccgtac
    28981 tcctcatcga gattgacacc ggaaatcgac tgctgctgat tggaaagctg cgtcaccaca
    29041 ttaccttgcg tggtgctact ggttttcaac gtcgcggttt tattaccgat atcactcact
    29101 aacgaggcat aagcgtcgtt aaaggatttc gctccgccca ccgttttact gttgctttgc
    29161 agatccagca gggcctgacc gttgcggttg tcgctatcac ccgcatcttc ttcgctcgcc
    29221 atcgctattt tcgcttcgtc ggtgattaat acatccatgt tgacgatggc gtcacttact
    29281 ggtttcagcg tgaagctgtc gttaacggca ggcgttcctg taaacgtcaa ctccagacca
    29341 tcaaatgcca ctttaccgtt ggcatctggc gtcaccgtaa aagtggtatt gctggcaagg
    29401 cgggtgacct gccactgatt attatcgaac gagattttgt aatctgtcgc cagtaccgcg
    29461 gaggcatcag ttaccgtggc accgatcgca acgttaccgt tgtttttagt gttttgcaga
    29521 accgcgggct taccgatagc aaagaaatct tcaccctcat cgccgttggc atcaaatccg
    29581 gctttgtgtt ggctgttgaa agcctcagca aatgccagcg ccagttgtcc aagcgtatta
    29641 cgcgtctggt ccagatcctg agaacggaat gtcagaatgc cgcccagcga cccggtattc
    29701 agtaatttct ccgggatctc aatattgcct gccgtcccat caacataagc gacagtcgta
    29761 cgagaagggt cggcgctgga aggaactgcc gccagttgcc gcgccgtact tccctgaacc
    29821 agtgagtaac cattggccat cgtgatgtta taagtgccgc catcctgaac gctgacttct
    29881 acaccaacaa tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta
    29941 ggtgacgccc ctgcccccac gcctgtcagg cgggagattt gatcgttcag gctggcgatt
    30001 tgtttagcgt aattgttgat ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg
    30061 tcctggtcgc gaagatactg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc
    30121 ccaatcagcg cctggcgcgc tgccgggtct tccgcgttac tcaccagcgt ttgcaggctg
    30181 gtgaagaaat cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg
    30241 attttcgaca tctgctcata gcgggcagtc aggccgctac tttgcgtctg cgccgcacgt
    30301 aactggttgg taataaacgc atcatactca cgctgcacac cagaaacgta gacaccatta
    30361 ccaacccagc cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg
    30421 gtatatccgg caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg
    30481 gccgcgttca gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag
    30541 ccttcaatta cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat
    30601 tatcaatgtt catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgaa
    30661 tcatgttggt gagtttacgg gcatagtgag gatcggtggc atagcccgcg tcctgtaggg
    30721 cctgcgcccc ctgctccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg
    30781 ttaacagccc aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaacttcg
    30841 cttttacttt cttcgcttcg ccattttcat attcagtcgt ggtgatttca gtgactggcc
    30901 ctttccagtt gccagaggct ttgacaccaa acaggttata gctcggctcg ccgttttcgc
    30961 ggcggatttg ccgttgcccc cagccagatt ccagcgccgc ctgagcgagg atcaaatgat
    31021 gtggcacacc gctttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg
    31081 ctttactgtc acctggcagc gaatcatcgt agttacgtgg cacggccttt tgcaccagct
    31141 gcgaaagcgc ctgattttga taacgcacca cggtttcgag cgggaatttc atcggtgctg
    31201 ctggcgtgga ctcctctggc aatggttgtt ctggcgtcat ctgcttaacc atcatctctg
    31261 caagccccag acctttgccc gtcgtcatct gttgggcaat ctgctggtca tacatactgg
    31321 tatacaggcg agtgtgctcg ctgctgaaca ggccatcttt tggtaacgcg tcgcgcatgc
    31381 ttttcaacat catctgcacg aacatccctt ccacctgacg ggccaccgga cggatatttg
    31441 ccgccggatc ttcgctcgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg
    31501 cacttgccag tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca
    31561 tcccgcactt tgcattgatt gcaaaataga catcagatcc atcggcgtag cgcccagcgc
    31621 attgagcgcg cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc
    31681 gccgctctgg cgtaagtcga tctgcgtttg tggcgttacc acggtctgtc cgccaccaaa
    31741 cggtgtatct ggttggctga cattggcctg acgattgact gttactgaga gatttccctg
    31801 cgctatcgcg cagctgtcga gagtcacttc gcgattcatc accaccgaac cggtgcgcga
    31861 gttaatcact actttagcgt cctgcggggt gacattaacc tgcatattct ggatatcggc
    31921 aaggaaacgg acctgggaac tgttgccact cggtacgcgc acctgaatag tccgcgcatc
    31981 caacgcggtg gcgctgccat atccacgcac gcggttgatg gtgtcagcga tttgctgcgc
    32041 catgctgaaa tcttcgtcgt taagttgcaa attaagggta ttcccgacgc caaactggct
    32101 gggtaattca cgttcaataa ctgcaccatt ggtgatccgt ccaccgttca gttggttcac
    32161 ctgaacactg ctaccgccag cggaggctcc tgcgccgcca accagaatat tgccctgcgc
    32221 cagcgcatac acctgactgt caacgccctt aagcggagtc atcaacaatg tgccgccacg
    32281 caggcttttg gcatttccca tggaagaaac caccacgtca atggtttgcc cctgacgtcc
    32341 aaacggtgga agtgacgccg tcaccattac cgcagcaacg ttttttagct gcatattggt
    32401 gcccgtcgga acggtaattc ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa
    32461 cggcgtctgg gttgtctggt caccggtgcc atccagcccc accaccagac catagccaat
    32521 cagtgagttt tgccttaccc cctgaacact ggtgagatcg cgaatacgct cagcctgagc
    32581 cgccgtcgta accagtagaa gaattaatgc agagagaaat ttaatcacca cagcctcact
    32641 tacattggcg acaggttaag gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta
    32701 atgtagccat tgcctacgta ttcaatgcgc gcatccgcca cctgagtaga cggtacggta
    32761 ttgctgccgc tgatagtgcg tggattaacc acgccagaga agcgaataaa ttcggtaccc
    32821 tgattaatgg caatctgttt ttcacccacc acatgcaggt tgccgttgac cagtacctgg
    32881 tcaaccgtca ccgtcaacgt gccgctaaag gtattactgg cattggcccc gccttttccg
    32941 ttgaacgtgt taccaccgga ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc
    33001 aaatagcgcg gcacagtatc aaagccaaaa ttagttttac cgtcacggct ggcattcgca
    33061 gaggagcttt tgctggcgct aacgttctcc tgcaacacga tggtcagcgt atcgccaata
    33121 ttgcgtggtc gacgatcttc aaacagcggt tgatagccat agttaatcgg ctgagcagac
    33181 tggaaaatag aaccgttggc gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc
    33241 ccctgcacca gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag caccaacaag
    33301 ctggaaatgg cataagtatg cgcagcgttt ttttgcatgg ctatcatctt caaaaatcaa
    33361 taaaccggtg aacctgccac cggttatgcc ttagagttgc gtcagttttt gcagcatctg
    33421 atcggtggtg gacaccgctt tactgttgat ttcgtaagcg cgttgcacct gaatcatatt
    33481 gaccagttct tccgccacgt tgacgttaga cgtttcaaca tacccttgat acagcagtcc
    33541 cgcgccgttc aggcccggcg tgctttcgtt cggtgcaccg gaggattgcg tttcggtgta
    33601 gaggttttcg ccaatgctct ccagcccggt gtcattcata aaggtggtga gattgagctg
    33661 cccaacctga accggagctg cctggccttg ttgggttacg ctgaccacgc catcacgacc
    33721 gatggtgata cttaacgcat tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc
    33781 agccgtcacc agctgcccgt tctgatccac ctggaaagag ccgtcacggg tataggctga
    33841 tgagccatct ggcaacatca cctggaaaaa gccctgccct ttaatcgcga catctttgct
    33901 gttgttggtc tgcgacaggt tgccttggct gtgtaagcgt tcagtggcga ccgggcgtac
    33961 ccccgtgccg atttgtaatc cggagggtaa ggtggtttgt tcggaagact gtgcccccgg
    34021 ctggcgaatg gtttgataaa gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt
    34081 agtactgacg tttgccaggt tgttggcaat gacgtccata ttggtttgct gggcgtcaag
    34141 gcccgttttg gcgatccata atgaactgat cataaaatgt cctgtagtgg attgctgaca
    34201 agcagagcag aagcacgcgg cggtgcgaat ttcccctcac cctaaccctc tccccaaagg
    34261 ggcgagggaa cagtccgctc gcttttattg caatgtcatc aatcgttcaa ttaactcatc
    34321 gacagcagtt ggttggcacg gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc
    34381 atttcaaaac gccgcgcgct ggcaatcatg tcgctcattg ccgcaacggc attgacgtta
    34441 ctgccttcca gaacccccga catcacacgc aaggttggat ctgcctgcag taccggtcca
    34501 cgcgtggcct gggtttctgc gcttaaacga aaaatgccgt cgtcaccgcg ctgcacttcg
    34561 ctgcccgtgg ctttcaccag tttcagacgc cccactggcg caaccgtatt tgccggatcg
    34621 cccggattca gcgccgagat tgttccatcg gcagctatcg tgatttccgc cccttccggc
    34681 acagcaattg gccccgcctc gcctatcacc ggatgcccct gaattgtcag ttgcccggtg
    34741 ggatcaacct gaatgctgcc attacgcgta tacccttcgc tgccgtcagc ggtctgcacg
    34801 gccaaccagc catcctgctg caacgcaacg tccagcgggc gcgaggtgta atccatcttg
    34861 ccgggcgtta tgtctgcacc cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa
    34921 agcccttcca ccggcactgc gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag
    34981 gcattggcca gattactggc ggttaccgcc tgttgattca gcgtctggct ggctgctccc
    35041 atcgcggtat aaattgcgtg atccattgag ctatcccgtc agcgattagc gtaagttaac
    35101 cagcgtgttg aggatctggt cctgggtttt gatggtctgg gcgttagact gatagttacg
    35161 ctgggcaacg atcatattga ccagttcttt actgagatcg acgttggacg cttccaacgc
    35221 accgttggtc agggtgccaa agttgcccgt cccggctgtc cccaacagcg ccacgccaga
    35281 agattgcgtc gcagaccaga cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa
    35341 gttcgccagt acaatctgcc ccagcagttg ggtttgttcg ttggaatagt tgccgacaac
    35401 cgtaccgtca tcattgattt gataactcac cagatcgccc ggtttgtagc cattctgggt
    35461 ggttgccaca atgttgttag cgccggtatt ttgctgcatg gagttgagga agctcaggct
    35521 aaacgtggcg ggttctgcgc cgttaattgc gccggtggta atattctctg ttggatttga
    35581 ggtcagaacg ccattggcat taaacaccag cttcattgca ggctcggctg tacctgttgg
    35641 atcactgcta tcctgggtgt agacctgcca gttattatcc ccggtcttca caaagtagac
    35701 gctcatgtca tgagcattac cctgactgtc gaaaacagtc accgaacctt ttttgttata
    35761 gctatccgca ttgctggcat caaatgcgtt aacagaggga agcggatcgc tggaattcag
    35821 gttgatctgc atcgacgccg tggtggtagt tttcgctgcc atcagggtat tcgggatcga
    35881 aatgttagtc ggattcgccc cttgctgaat agtcggcggc gtaccggttg ccgggtaacc
    35941 cgtcagctgt aaaccttgca tattcaccag gttacgattt tcatccagct taaattgtcc
    36001 gttacggctg tagaacaccg aaccgttgct atctaccaga cggaaaaaac cgttctggct
    36061 gatagcaacg tccagaccac gcccggtgtt ggtggtcgtg ccatcggtaa agtcctgagt
    36121 gataccggca acttttaccc ccagtcccac tttcgaaccg gcaaacatat cggcaaaaga
    36181 ggccgtgcct gatttaaagc cgtaggtggc ggagttggcg atattgttgc caataacatc
    36241 gaggttggtg gcggcagcgt ttaatccgct aaccgcttga gaaaaggcca tgactgactc
    36301 ctgaaatgtg aaggcttaaa ttatctgccg tacttcgtcg agggtggtgg tgccgtaagt
    36361 gccgagatcc agcgtattac cgttgttgcc gcggatcaca ccctgcacca gagcaaactg
    36421 cagcggctgg gcaaccagtt gtgtaccacc gttactggcg ctaatcgcta cgttgtaaga
    36481 accgttcggc gcagtgctgc cgtcagtcaa cgtaccgtcc caggtgaaac tgtgaactcc
    36541 ggcggtcagt tcaccaatat caatggtgcg cacaaccgcg ccatttttat cggtgatggt
    36601 ggccgtaact ttgtctgccg cctgttgcag ctcaacacca aacggcgtgg tcgtggtcac
    36661 agccccttct tcactgccgg ttcccgcaag aacagtggtg ccggggatca tcacgccgtg
    36721 accgatcagg ttactggcct gtaacgactg gctgttatca atctgtccgg aaatagatcc
    36781 gagcgtggta ttgagttttt caatcccgct gaccgtgctg atttgtgcca attgcgacgt
    36841 cagctcgttg ttttccattg gattggtcgg gtcctggttt ttcagctgcg ccaccaacaa
    36901 agtcagaaaa ctgctttgta aatctgcggc gttgctgccc gtgagcgaac tactgctggt
    36961 ggtactgacg ccggtatttg tcggatcggt ggtggttacc gcaatggaca tagctttctc
    37021 ctttattgac cgagcgtaag ggttttcagc atcatgcttt tcaccgtgtt gagcacttca
    37081 acattggcct gatagctgcg tgacgccgac atggtgttaa ccatctctcc gacaacatca
    37141 acgttcggca tttttacgta gccctttgca tccgccagcg gattacccgg ttcataaacc
    37201 agtttgtccg gggcctgact ttctataaca tcggcaacct ttacgccgcc tgtcgcagca
    37261 cctggtgcag cgttaacctg gaataccacc tgttttgccc gatatggctg tccatcagga
    37321 ccggtcacgc tatcagcatt cgccagatta ctggccgcca cgttcaggcg ctgggactgg
    37381 gcagttaacg ccgacccggc gatatcaaaa atattcagca gtgccatccg ttaatttccg
    37441 ctctgtaaaa cgttcatcat gcctttgatt tgcccgctca acgcgctaag gctcatctgg
    37501 tattgcaggc tgttatcggc aaactgggtg cgttcgcgat ccatatcgac ggtattaccg
    37561 tcaagcgaag gctggtccgg aatacggtat tgcagttctg cggtaggagg cgtcagcgcc
    37621 tgcgccggaa tgtgttgcgt tgaggtcatc gtcagagcaa ccacactggt tgcattccgt
    37681 ccacgttgca tgactttttt aagttcactg gcaaaatcga tatcgcgcgc ctgataacca
    37741 ggggtatcgg cattggcgat gtttgctgcc agcacttcct gacgctgggc gcgcagattg
    37801 agcgcctctt gttgaaaacg taaggcggcg tcgagcttat cgagcatatc tcctccgcag
    37861 gtatcaaaat tctgccatca gcttaaatgc ctttacgcgc gcgttatcgg cggaataaac
    37921 gcaaaatggg tcgctattta tgccgttgat ggtcattgtg gacaggtaca attcacgttg
    37981 tagaaatggc tgggggcgaa aatgctggca ataaaacgta gcgtggcgat catcgcgata
    38041 ctgttcagtc cgttaagtgc ggcgagcaat ctcacatcgc aattgcaaac cttttttagc
    38101 tcccagctcg cagggataag tgatgaggtt cgtgtttcta ttcgtacagc gcccaatcta
    38161 ctaccgccgt gcgagcagcc attgctttcg atgagcaata attcccgctt gtggggcaat
    38221 gtgaatgtgt tggcacgctg cggtaacgac aaacgatatt tacaggttaa tgtgcaggcc
    38281 acaggaaatt atgtggttgc cgcgatgccc attgtgcgag gaggaaagct gggagctggc
    38341 aatgtcaaac tgaaacgcgg acggctggat accttgccac cgcgtacggt gctggatatc
    38401 aatcaacttg ttgatgccgt tagcctgcgc gatctatcac ccgatcaacc tatccagtta
    38461 acccagtttc gccaggcatg gcgggtaaaa gcgggacaac gcgtcaacgt gatcgccagc
    38521 ggtgatgggt ttagcgccaa cgcagaaggt caggcgctaa acaatgcagc cgtcgcacag
    38581 aatgcgcggg tgcgcatggt atctggacag gtagtcagcg gcgttgttga tgcagatggg
    38641 aatattctta taaacctgta agctgttaaa gattacccgt cccttgccga taaataagca
    38701 acacatgata aaagcgccct caatgaggaa taaaccatga gtattgatcg cacttcgcct
    38761 ctgaagcccg taagcaccgt tcaaccgcgc gaaaccactg acgcgccggt aacgaacacc
    38821 cgagcggcaa aaacaacagc ctccaccagc accagtgtga cgttaagcga cgcgcaagca
    38881 aaactgatgc aacccggcag cagtgatatc aatcttgaac gtgtcgaagc gttaaaactg
    38941 gcgattcgta acggtgaact aaaaatggac accggcaaaa ttgccgatgc gctgatcaac
    39001 gaagcgcagc aagacttgca gagtaattaa tcgtatgacg cgtcttgcag agatcctcga
    39061 ccagatgtcc gctgtgctta acgatctcaa aacggtaatg gatcaagagc agcaacatct
    39121 ctctatgggg cagatcaacg gcagccagtt gcaatggatt acagaacaaa aaagctcact
    39181 gctggcaacg ttggattacc tcgaacagtt acgcaggaaa gaacccaata cagcaaatag
    39241 cgttgatatt agtcaacgct ggcaggaaat tgctgtgaaa acgcagcaac tacgccaaat
    39301 gaatcaacat aacggctggt tactggaagg acagattgag cgcaatcaac aggcgctgga
    39361 aatgttgaaa ccgcatcagg aaccgacgct atatggggcg aacggtcaga cctcaacaac
    39421 ccatcgcggc agtaaaaaga tttcgatctg aagattatct ccggcctgca ctgcaggccg
    39481 gaatgcattg ttacaccgtc cggcgggcaa attctttaac tttgaagccc agtaccgcca
    39541 gtgcagcgaa gtacgcggca atccccgcca gcacgaccgc cattaaacgc agcaaacgcc
    39601 agggcatggt acccaatgac cactccggca tgatatgtaa catacctaaa agcacgccag
    39661 acatcaccag taccgccacc accagacgca acagaaacgc catccagcca ggttgcgggg
    39721 taaagatttt ctgcttacgc aactgccagt aaagcagcga agcattcaga cacgccgcca
    39781 gaccaataga aagtgacagc ccggcatgtt tcaacggacc gataaacgcc aggttcatca
    39841 attgcgttaa aatcagcgta acgatggcaa ttttcactgg cgttttaatg tcctggcggg
    39901 aataaaagcc aggagccaac actttcacta caatcaggcc aatcaaaccc accgagtagg
    39961 caattaacgc ccgctgggtc atcagcgcat caaaggcggt aaatttaccg tactggaaca
    40021 gcgaaacggt taacggaccg gaaagaatgc ccaacgcaac cgcactcggc aacgccaaca
    40081 ggaaacaaag acgcagtccc cagtccatca aacggttgta ttcatcatga ttgccactgg
    40141 caaaactttt cgacagcgac ggtagcaaaa tggtgccaag cgccacgccc agcacaccgg
    40201 acggaaactc cattaagcgg tcggcgtaat acatccatga caccgaaccg gaagcaagaa
    40261 acgaggcaaa aatggtgttg atgattaagg agatctggct cacagagacg ccaaggattg
    40321 ccggtcccat ctgtttcacc acacgcattg ctccggcatc gtggaagtta atgcgtggca
    40381 aaaccagcat gccgatcttc ttcagatgcg gtagctgata caccagctgc aggacgccgc
    40441 cgaccgtcac agcccacgcc agcgccagca ccggtgggtt aaagtacggt gcggcaaaca
    40501 gcgcgaaacc aatcatgctg atgttaagca gtgttggagc aaacgccgga atcgagaagc
    40561 ggttccacgt attcagaatc gctcccacca gcgacgccag ggagatcagc aagatatagg
    40621 gaaaggtaat ctttagtagc tggctggtca gggcaaattt gtcagctgtg tcagcgaagc
    40681 ctggcgcggt caccatgatc acccacggtg cggcgagcat gccagcgacc gtcacaaccg
    40741 ccagcgcaag tgtcagcagg ccagaaacat aagagacaaa gacccgcgtg gcgtcttcac
    40801 cctgcttact tttatattcc gccagaatcg gtacaaatgc ctgggaaaat gccccttcgg
    40861 caaagatacg gcgtaacaag ttaggaagtt taaaagcgac gaaaaaggcg tcggttgcca
    40921 tccctgcgcc aaagattctg gcgacaattg cgtctcgtgc gaagccaagc acacgcgaaa
    40981 acatggtcat cgagctgacg gcggccagcg attttaataa attcatcggt gttctaatcc
    41041 agacccacgg gcaaaacgcc tgcaaggcag gcgctgaaag aggcgaatag tctacctgga
    41101 ttatggtgaa ttgctaccgc cagatgttac agggtttatt cactcatcgc atcgcgccag
    41161 atcttgtcaa cgatacgttg cgccagcact gcctgttcgc cggcggtttg cggaactgtc
    41221 tgattttgca cgcattcaat gaagtgccgc gcacagccga caaatccacg ttgctcaagc
    41281 gtgctctgcc aaccaggaat cggtttattc actacaccct gcccacgctc ctcacgccat
    41341 tcgcgcattt ccgtaatgtc gatgagcgca ccgtcagtca cggcctgcac ggtttcacgc
    41401 tgacttccgg cacggcgatg catacaagtg gtgatctgca aaggaccggc cgaaaaatgg
    41461 tgctcggcaa acagcatttc gccagcgtcg ttagtcagta gcgtaccgcc atccagagag
    41521 gctttgccgc ccgacaacca cagcgcggta tccaccacat gcagataatc atccagcaac
    41581 gtgaaataaa gatcgtgtgg cccgacgcta ttgctacgat gtttatccat tcttagcgag
    41641 gctgcggtgg cgagttgcgt ttttaactca cagtagagtg gtgcgaaacg acggttaaaa
    41701 ccgaccatca acgtcagttt tttccgcgcc gccagttcca ccagccgttc agcatcgcgc
    41761 agattttctg ccagcggttt atcgacacag acatgtaccc ccgcattgag taacgtactg
    41821 accacgtcaa agtgactggc ggtgctggaa tgtacaaaaa ccgcatcgca actggcggca
    41881 aggctggata acgaatcggc ataaggaatg cgccagcttt cacaaattgg cagggctttc
    41941 gcgcgcgtag gcgaccaggc tccttgtaac gtccagtcag acgctgccgc cagcaccggt
    42001 aaccacgctt tttgcgcaat gccgcctaat cccactacgc cgatacgtaa ttttttcact
    42061 ttaatctccc aggtgggcca gcaacgaatc aagacgctgt ttcagttctg ccacttcgat
    42121 ttccagggct tcgacgcggg cctgtaaatc accgtcaacc gcgttcgaca tatccgttac
    42181 cgccggctga tcctctacct caccactgaa aagatgcatg tagcggcttt cgcgtttacc
    42241 cggttcgcgg gccagacgca ccacaaaagg accatcttcg cgattcgcca gttgttccag
    42301 cgtcaactcc acttccgcca tatcgctgaa ttcatacatt cgcgcggcgc ggctgcgcag
    42361 ttcacccggc gtctgggcac cacgcaataa caacgtggtg attaacgcca cttctgcggc
    42421 gctcagtttc agatcgccaa attctgaatt acaaaaacgt tgctcatatt tggtgacccg
    42481 attaccaaaa ccgctcactg tgcgtagata atgacgtttg accagattat ccagctgttc
    42541 ctgcacttca gattcactca gattcatgac cggttcacgg ttcgttttct gattacaggc
    42601 cgtgactaca ccattgactg agagcgggta ttgctccggc gtggtcacct gcttttccag
    42661 caagcaacca atcacacgag cttccagggc agtaagttga tatttcatga gattctcctt
    42721 agcggccggg cgtccagtct ggggtagtta atgccgtcag tacgtgatcg cgccattgtc
    42781 catcaatcaa cagatagtct ttcgcatagc cttctttttc aaaacccagt cgcgccagta
    42841 aatcaccgct gcgtttattg tgcggcatat aattagccat aatgcgatga atatgttggg
    42901 tgcgctgcat ataacgaatg gctgcggtca gggcttcaaa catgagtcct ttgccctgcc
    42961 atttttgccc aatcgaataa ccgagatagc aggcatgaaa agagccgcga acaacattgg
    43021 aaaaattggc aacgccaata atctcttttt cgtccgggtc gaataagcca aagtagaaag
    43081 ctgaaccttg tttatgaaat tcgttaatca tccccagcct ggcctgccag cctgatggat
    43141 aacagtggct ttcgtcgcgc actggctccc agggcttgag gaaatggcga ttctctgcgt
    43201 aataatccgc cagacgccag gcatcacgat catgcaccag acgcacgacc agtcggtctg
    43261 tggttaagcg cacttttggc acgttactgc gatagccaaa cattctatac ctactccttc
    43321 ccgtaacgtc tttaatacgc tgtaagtatt actatacctg cgcaaccaga ccctgtgaaa
    43381 agtccaaaat gccattttct gcattattca catatttgat aaaaactctc tatcaaagcg
    43441 acgttttgat aaaaaaatat tgtccccgaa gtgatgggaa aagtcttagc ggcaatgcag
    43501 aatagaaagt gctcatcttt tatttcccgg gaggggaaat gtcccgcgtg tcgcaggcga
    43561 ggaacctggg taaatatttc ctgctcatcg ataatatgct ggtcgtgctg gggttctttg
    43621 ttgtcttccc gctgatctct atccgcttcg ttgatcaaat gggctgggcc gccgtcatgg
    43681 tcggtattgc cctcggccta cgccagttta ttcagcaagg tctgggtatt ttcggcggcg
    43741 caattgccga ccgctttggt gccaaaccga tgattgttac cggtatgctg atgcgtgccg
    43801 ccggattcgc cacaatgggt atcgcccacg aaccgtggct attgtggttt tcatgcctgc
    43861 tctcgggact cggtggcacg ttgtttgatc cgccgcgttc ggcgctggtg gtgaaactaa
    43921 tccgtccaca acagcgtggt cgttttttct cgctgttgat gatgcaggac agtgccggtg
    43981 cggtcattgg cgcattgttg gggagctggc tgttgcaata cgactttcgc ctggtctgcg
    44041 ccacaggggc cgttttgttt gtgctgtgtg cagcgttcaa tgcgtggttg ttaccggcat
    44101 ggaaactctc caccgtacgc acgcccgttc gcgaaggcat gacccgcgtg atgcgcgaca
    44161 agcgttttgt cacctatgtc ctgacgctgg cgggttacta catgctggct gtacaagtga
    44221 tgctgatgct gccaattatg gtcaacgatg tggctggcgc gccctctgcc gttaaatgga
    44281 tgtatgccat tgaagcgtgt ctgtcgttaa cgttgctcta ccctatcgcc cgctggagtg
    44341 aaaagcattt tcgtctggaa caccggttga tggctgggct gttgataatg tcattaagca
    44401 tgatgccggt gggcatggtc agcggcctgc aacaactttt caccctgatt tgtctgtttt
    44461 atatcgggtc gatcattgcc gagcctgcgc gtgaaacctt aagtgcttcg ctggcggacg
    44521 caagagctcg cggcagctat atggggttta gccgtctggg tctggcgatt ggcggcgcta
    44581 ttggttatat cggtggcggc tggctgtttg acctgggcaa atcggtgcac cagccagagc
    44641 ttccgtggat gatgctgggc attattggca tcttcacttt ccttgcgctg ggttggcagt
    44701 ttagccagaa acgcgccgcg cgtcgtttgc ttgaacgcga cgcctgattt gatactcccc
    44761 cctgactcct ccatactgaa agtgaactga cagccaaatg gaggagtcac gtgaagctat
    44821 acatttacga tcactgccct tactgcctca aagcccgcat gattttcggc ctgaaaaata
    44881 tccccgtcga attacatgtt ctgctcaacg acgacgcaga aacacccacc cggatggtcg
    44941 gtcaaaaaca ggttcccatt ctgcaaaaag atgacagccg ctatatgcca gaaagcatgg
    45001 atatcgttca ctatgtcgat aaactcgacg gcaaaccgtt actgaccggc aaacgttccc
    45061 ctgccattga agagtggctg cgcaaggtca atggctacgc caacaaactg ctgttgccgc
    45121 gttttgccaa atcggcattt gatgagtttt ctactcccgc cgcgcgcaaa tatttcgtcg
    45181 acaagaaaga ggccagcgcg ggtaattttg ccgacctgct ggcccactct gacggtctga
    45241 ttaagaatat cagcgatgat ttacgtgcgc tggacaaact gatcgtcaaa ccgaacgccg
    45301 tgaatggcga actttcggaa gatgatattc agctattccc gctactgcgt aatctgacgc
    45361 tggtagccgg aattaactgg ccaagccgcg ttgctgatta ccgcgataat atggcgaaac
    45421 agacacaaat caatttgtta tcatcaatgg cgatttaatc ctgactcgcc gcggcagcct
    45481 gtattcgcgg gttgccgccg ttttcagttt tttctgctgg cgagtttatc gctggtgaca
    45541 catgctgcgt tgtgtcagga tattgacgca gatttcatgt cgaggactac catgaacaag
    45601 tttttatttg ccgctgcatt gatcgtcagt ggcctgctcg ttggctgtaa tcaactcacc
    45661 caatacacca tcaccgaaca agaaattaac cagtcgcttg cgaaacataa taatttctca
    45721 aaagatattg gtttacccgg tgtggctgac gcccatattg ttctgacaaa cctgaccagc
    45781 caaattggtc gcgaagagcc gaataaggtt gccctaaccg gagacgccaa tctggacatg
    45841 aactccctgt tcggtagcca gaaagcgacc atgaaactga agctgaaagc gctgcccgtg
    45901 tttgataaag aaaaaggcgc gatcttcctg aaagagatgg aggtggtcga tgcgacggta
    45961 caaccggaaa aaatgcaaac ggtgatgcaa acgttgcttc cctatctgaa ccaggcatta
    46021 cgcaattact ttaaccagca acctgcttac gtcctgcgcg aagatggcag ccagggcgaa
    46081 gcaatggcga aaaaactggc gaaaggcatt gaagtgaagc caggcgaaat tgtcattcca
    46141 tttactgatt aatcacgagg gcgcattcgc gccctttatt tttcgtgcaa aggaaaacgt
    46201 ttccgcttat cctttgtgtc cggcaaaaac atcccttcag ccggagcata gagattaatg
    46261 actgcaccat cccaggtatt aaagatccgc cgcccagacg actggcacct tcacctccgc
    46321 gatggcgaca tgttaaaaac tgtcgtgccg tataccagcg aaatttatgg acgggctatc
    46381 gtaatgccca atctggctcc gcccgtgacc accgttgagg ctgccgtggc gtatcgccaa
    46441 cgtattcttc acgccgtacc tgccgggcac gatttcaccc cattgatgac ctgttattta
    46501 acagattcgc tggatcctaa tgagctggag cgcggattta acgaaggcgt gttcaccgct
    46561 gcaaaacttt acccggcaaa cgcaaccact aactccagcc acggcgtgac gtcagttgac
    46621 gcaatcatgc cggtattaga gcgcatggaa aaaatcggta tgccgctact ggtgcatggt
    46681 gaagtgacac atgcagatat cgacattttt gatcgtgaag cgcgctttat agaaagcgtg
    46741 atggaacctc tgcgccagcg cctgaccgcg ctgaaagtcg tttttgagca catcaccacc
    46801 aaagacgcag ccgactatgt ccgtgacgga aatgaacggc tggctgctac catcactccg
    46861 caacatctga tgtttaaccg caaccatatg ctggttggtg gcgtgcgtcc gcacctgtat
    46921 tgtctaccca tcctcaaacg caatattcac caacaggcat tgcgtgaact agtcgccagc
    46981 ggttttaatc gagtattcct cggtacggat tctgcgccac atgcacgtca tcgcaaagag
    47041 agcagctgcg gctgcgcggg ctgcttcaac gccccaaccg cgctgggaag ttacgctacc
    47101 gtctttgaag agatgaatgc tctgcagcac tttgaagcat tctgttctgt aaacggcccg
    47161 cagttctatg ggttaccggt caacgacaca ttcatcgaac tggtacgtga agagcatcag
    47221 gttgctgaaa gcatcgcact gactgatgac acgctggtgc cattcctcgc cggggaaacg
    47281 gtacgctggt ctgttaaaca ataaaaatcg tagcttcctg ttgtcattag gttattttac
    47341 ctgtataaat aaccagtata ttcaacaggg ggctattatg cgaattgaag tcaccatagc
    47401 gaaaacttct ccattgccag ctggggctat tgacgccctg gctggcgaac tttcccgccg
    47461 tattcagtat gcgtttcctg ataatgaagg ccacgtatca gtacgttatg ccgcagcgaa
    47521 taatttatcg gttattggcg cgacaaaaga agataaacag cgcattagcg aaattctcca
    47581 ggaaacgtgg gaaagcgccg atgactggtt tgtcagcgaa taatatgcag tgatttttgt
    47641 ttttgccggg tcgccccggc ttttttattt tacttgcgaa ttatgagtac aaaatttcta
    47701 cacttcctca aaataattgc acaatatgct aaaaaaatat gaatgacatg ctgttttatt
    47761 gttcatataa atcttaaaa
//
LOCUS       DAZKIF010000017        45735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZKIF010000017 DAZKIF010000000
VERSION     DAZKIF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.017"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            158..784
                     /gene="ydhY"
                     /locus_tag="ACHOTW_001033"
     CDS             158..784
                     /gene="ydhY"
                     /locus_tag="ACHOTW_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP9843191.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            805..2907
                     /gene="ydhV"
                     /locus_tag="ACHOTW_001034"
     CDS             805..2907
                     /gene="ydhV"
                     /locus_tag="ACHOTW_001034"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP9843192.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2920..3558
                     /gene="ydhW"
                     /locus_tag="ACHOTW_001035"
     CDS             2920..3558
                     /gene="ydhW"
                     /locus_tag="ACHOTW_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP9843193.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3622..4290
                     /gene="ydhX"
                     /locus_tag="ACHOTW_001036"
     CDS             3622..4290
                     /gene="ydhX"
                     /locus_tag="ACHOTW_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9843194.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4287..5072
                     /gene="phsC"
                     /locus_tag="ACHOTW_001037"
     CDS             4287..5072
                     /gene="phsC"
                     /locus_tag="ACHOTW_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP9843195.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5076..5888
                     /gene="ydhT"
                     /locus_tag="ACHOTW_001038"
     CDS             5076..5888
                     /gene="ydhT"
                     /locus_tag="ACHOTW_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP9843196.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5900..7504)
                     /gene="ydhS"
                     /locus_tag="ACHOTW_001039"
     CDS             complement(5900..7504)
                     /gene="ydhS"
                     /locus_tag="ACHOTW_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP9843197.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7630..7935)
                     /gene="ydhR"
                     /locus_tag="ACHOTW_001040"
     CDS             complement(7630..7935)
                     /gene="ydhR"
                     /locus_tag="ACHOTW_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP9843198.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8043..8119)
                     /locus_tag="ACHOTW_001041"
     tRNA            complement(8043..8119)
                     /locus_tag="ACHOTW_001041"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8083..8085),aa:Val,seq:gac)
     gene            complement(8124..8200)
                     /locus_tag="ACHOTW_001042"
     tRNA            complement(8124..8200)
                     /locus_tag="ACHOTW_001042"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8164..8166),aa:Val,seq:gac)
     gene            8508..9764
                     /gene="ydhQ"
                     /locus_tag="ACHOTW_001043"
     CDS             8508..9764
                     /gene="ydhQ"
                     /locus_tag="ACHOTW_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP9843199.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9805..11178)
                     /gene="mdtK"
                     /locus_tag="ACHOTW_001044"
     CDS             complement(9805..11178)
                     /gene="mdtK"
                     /locus_tag="ACHOTW_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP9843200.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            11393..12034
                     /gene="ribE"
                     /locus_tag="ACHOTW_001045"
     CDS             11393..12034
                     /gene="ribE"
                     /locus_tag="ACHOTW_001045"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP9843201.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQSGTEA"
     gene            complement(12074..13222)
                     /gene="cfa"
                     /locus_tag="ACHOTW_001046"
     CDS             complement(12074..13222)
                     /gene="cfa"
                     /locus_tag="ACHOTW_001046"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP9843202.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13513..14724)
                     /gene="punC"
                     /locus_tag="ACHOTW_001047"
     CDS             complement(13513..14724)
                     /gene="punC"
                     /locus_tag="ACHOTW_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP9843203.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14837..15769
                     /gene="punR"
                     /locus_tag="ACHOTW_001048"
     CDS             14837..15769
                     /gene="punR"
                     /locus_tag="ACHOTW_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP9843204.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15766..16791)
                     /gene="purR"
                     /locus_tag="ACHOTW_001049"
     CDS             complement(15766..16791)
                     /gene="purR"
                     /locus_tag="ACHOTW_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP9843205.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17090..17179
                     /gene="cydH"
                     /locus_tag="ACHOTW_001050"
                     /gene_synonym="ynhF"
     CDS             17090..17179
                     /gene="cydH"
                     /locus_tag="ACHOTW_001050"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP9843206.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17345..18514
                     /gene="ydhP"
                     /locus_tag="ACHOTW_001051"
     CDS             17345..18514
                     /gene="ydhP"
                     /locus_tag="ACHOTW_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9843207.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18660..19241)
                     /gene="sodB"
                     /locus_tag="ACHOTW_001052"
     CDS             complement(18660..19241)
                     /gene="sodB"
                     /locus_tag="ACHOTW_001052"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP9843208.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19369..20184)
                     /gene="mepH"
                     /locus_tag="ACHOTW_001053"
     CDS             complement(19369..20184)
                     /gene="mepH"
                     /locus_tag="ACHOTW_001053"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP9843209.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SLTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20519..20866
                     /gene="grxD"
                     /locus_tag="ACHOTW_001054"
     CDS             20519..20866
                     /gene="grxD"
                     /locus_tag="ACHOTW_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP9843210.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20917..25533)
                     /gene="lhr"
                     /locus_tag="ACHOTW_001055"
                     /pseudo
     CDS             complement(20917..25533)
                     /gene="lhr"
                     /locus_tag="ACHOTW_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(25626..26273)
                     /gene="rnt"
                     /locus_tag="ACHOTW_001056"
     CDS             complement(25626..26273)
                     /gene="rnt"
                     /locus_tag="ACHOTW_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP9843211.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26376..26783)
                     /gene="gloA"
                     /locus_tag="ACHOTW_001057"
     CDS             complement(26376..26783)
                     /gene="gloA"
                     /locus_tag="ACHOTW_001057"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9843212.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26864..27961)
                     /gene="nemA"
                     /locus_tag="ACHOTW_001058"
     CDS             complement(26864..27961)
                     /gene="nemA"
                     /locus_tag="ACHOTW_001058"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP9843213.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(27998..28597)
                     /gene="nemR"
                     /locus_tag="ACHOTW_001059"
     CDS             complement(27998..28597)
                     /gene="nemR"
                     /locus_tag="ACHOTW_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP9843214.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            28700..28939
                     /gene="ydhL"
                     /locus_tag="ACHOTW_001060"
     CDS             28700..28939
                     /gene="ydhL"
                     /locus_tag="ACHOTW_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP9843215.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            28988..29884
                     /gene="ydhF"
                     /locus_tag="ACHOTW_001061"
                     /pseudo
     CDS             28988..29884
                     /gene="ydhF"
                     /locus_tag="ACHOTW_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            29965..30486
                     /gene="sodC"
                     /locus_tag="ACHOTW_001062"
     CDS             29965..30486
                     /gene="sodC"
                     /locus_tag="ACHOTW_001062"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP9843216.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30487..32499)
                     /gene="ydhK"
                     /locus_tag="ACHOTW_001063"
     CDS             complement(30487..32499)
                     /gene="ydhK"
                     /locus_tag="ACHOTW_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9843217.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32499..33356)
                     /gene="ydhJ"
                     /locus_tag="ACHOTW_001064"
     CDS             complement(32499..33356)
                     /gene="ydhJ"
                     /locus_tag="ACHOTW_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9843218.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33359..33595)
                     /gene="ydhI"
                     /locus_tag="ACHOTW_001065"
     CDS             complement(33359..33595)
                     /gene="ydhI"
                     /locus_tag="ACHOTW_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP9843219.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33796..34230
                     /gene="slyA"
                     /locus_tag="ACHOTW_001066"
     CDS             33796..34230
                     /gene="slyA"
                     /locus_tag="ACHOTW_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP9843220.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34277..34744)
                     /gene="slyB"
                     /locus_tag="ACHOTW_001067"
     CDS             complement(34277..34744)
                     /gene="slyB"
                     /locus_tag="ACHOTW_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP9843221.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35017..36126
                     /gene="anmK"
                     /locus_tag="ACHOTW_001068"
     CDS             35017..36126
                     /gene="anmK"
                     /locus_tag="ACHOTW_001068"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP9843222.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            36224..36553
                     /gene="mliC"
                     /locus_tag="ACHOTW_001069"
     CDS             36224..36553
                     /gene="mliC"
                     /locus_tag="ACHOTW_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP9843223.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36612..37268
                     /gene="pdxH"
                     /locus_tag="ACHOTW_001070"
     CDS             36612..37268
                     /gene="pdxH"
                     /locus_tag="ACHOTW_001070"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP9843224.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37397..38671
                     /gene="tyrS"
                     /locus_tag="ACHOTW_001071"
     CDS             37397..38671
                     /gene="tyrS"
                     /locus_tag="ACHOTW_001071"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9843225.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38733..39593
                     /gene="pdxY"
                     /locus_tag="ACHOTW_001072"
     CDS             38733..39593
                     /gene="pdxY"
                     /locus_tag="ACHOTW_001072"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFP9843226.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(39637..40242)
                     /gene="gstA"
                     /locus_tag="ACHOTW_001073"
     CDS             complement(39637..40242)
                     /gene="gstA"
                     /locus_tag="ACHOTW_001073"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP9843227.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40348..41850)
                     /gene="dtpA"
                     /locus_tag="ACHOTW_001074"
     CDS             complement(40348..41850)
                     /gene="dtpA"
                     /locus_tag="ACHOTW_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP9843228.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42461..43096)
                     /gene="nth"
                     /locus_tag="ACHOTW_001075"
     CDS             complement(42461..43096)
                     /gene="nth"
                     /locus_tag="ACHOTW_001075"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9843229.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43096..43791)
                     /gene="rsxE"
                     /locus_tag="ACHOTW_001076"
     CDS             complement(43096..43791)
                     /gene="rsxE"
                     /locus_tag="ACHOTW_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP9843230.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43795..44415)
                     /gene="rsxG"
                     /locus_tag="ACHOTW_001077"
     CDS             complement(43795..44415)
                     /gene="rsxG"
                     /locus_tag="ACHOTW_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP9843231.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            complement(44419..45477)
                     /gene="rsxD"
                     /locus_tag="ACHOTW_001078"
     CDS             complement(44419..45477)
                     /gene="rsxD"
                     /locus_tag="ACHOTW_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP9843232.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45478..>45735)
                     /locus_tag="ACHOTW_001079"
     CDS             complement(45478..>45735)
                     /locus_tag="ACHOTW_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP9843233.1"
                     /translation="EAAITRAKARKLEQQQANAEPEEQVDPRKAAVAAAIARAKARKL
                     EQQQANAEPEEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
BASE COUNT        11506 a        11314 c        11913 g        11002 t
ORIGIN      
        1 aaaggattag tttatcggcg ttaaatattt tctcatacac tatcgggtta gggaaaatat
       61 ctggtgttgg ggacagagtc agcagcgtat tagttgaatg cttaatatca ggctgctgaa
      121 ttgtcagcac tgtgtttgcg aagaggatgc gaaataaatg aacccggttg atcgtccact
      181 attagatatt ggcttaacgc gattagaatt cttgcgtata tcaggaaaag gccttgctgg
      241 tctaacaatt gcccctgcgt tactgtcact tttgggttgc aaacaggaag atattgatag
      301 cggcacagta gggttgataa atacacccaa aggggtgctg gtgacccagc gagcgcgatg
      361 cacgggttgc caccgctgtg aaatctcttg taccaacttc aacgatggct cagtagggac
      421 attcttctcc cgtatcaaaa tccatcgcaa ttatttcttt ggcgacaacg gggttggctc
      481 tggcggcggc ctgtatggcg atctcaacta taccgcggac acctgccgtc aatgcaaaga
      541 gccgcaatgc atgaacgtct gcccgattgg tgcgattacc tggcagcaga aagaaggctg
      601 tattaccgtc gatcataaac gttgtattgg ctgtagcgcc tgtactacgg catgcccgtg
      661 gatgatggcc accgtaaata ccgaaagtaa aaaatcctcg aaatgtgtgt tatgcggtga
      721 atgcgcaaac gcctgcccaa caggggcgtt aaaaattatc gagtggaaag atattactgt
      781 gtgaatcttg caaaggaaaa aatcatgact aacggttgga caggtaatat attaagagtc
      841 aatctcacga caggaaatat taccctcgaa gattccagta agtttaaaag ttttgtcggt
      901 ggcatgggct tcggctacaa aattatgtat gacgaagtac cgccaggcac gaaacctttc
      961 gatgaagcga ataaattagt ctttgctacc ggcccattaa ctggatctgg tgccccctgt
     1021 agttctcgcg taaatatcac ctcactttct acttttacca aaggaaattt agtcgtcgat
     1081 gcccatatgg gtggcttttt tgcagcgcaa atgaaattcg ctggatacga cgtcattatt
     1141 atcgaaggga aggcgaaatc accggtatgg ctgaagatta aagatgacaa agttagcctg
     1201 gaaaaagcag atttcttatg gggaaaaggg acgcgcgcaa cgacggaaga aatttgtcgc
     1261 ttgaccagtc cggaaacctg tgtggcggct attggtcagg ctggggaaaa ccttgttcct
     1321 ctctctggca tgttgaatag ccgtaaccac agcggcggtg cgggaactgg cgcaataatg
     1381 ggttcgaaaa acctgaaagc gattgcggtt gaagggacga aaggggtcaa cattgccgat
     1441 cgtcaggaga tgaagcgtct caatgattac atgatgactg aacttatcgg tgcgaataac
     1501 aaccatgtcg tgccaagtac gccgcaatcg tgggcagagt attcagatcc caagtcacgc
     1561 tggacagcac gtaaagggct gttttggggc gcggctgaag gcggtccaat tgaaacgggt
     1621 gaaattccgc caggcaatca gaatacggtc ggctttcgta cctataaatc cgtttttgac
     1681 ttaggtccgg cagcagagaa atacacagta aaaatgagcg gctgccactc ttgcccgatc
     1741 cgttgtatga cccaaatgaa tattcctcgg gtgaaagagt ttggcgtgcc cagcacaggt
     1801 ggtaacactt gtgtagcaaa ctttgtccat accaccatct tcccgaacgg gccgaaagat
     1861 tttgaagata aagacgatgg tcgtgtgatt ggtaacctgg ttggtctgaa tttgttcgat
     1921 gactacggcc tatggtgtaa ctacgggcag ttgcatcgcg actttactta ttgttacagc
     1981 aaaggtgtgt tcaagcgtgt tctgcctgct gaagagtatg cagaaattcg ctgggatcaa
     2041 ctggaagcgg gagacgttaa cttcattaaa gatttttact accgtctggc gcatcgtgtg
     2101 ggtgagctga gtcacctggc tgatggttca tatgccatcg cagaatgctg gaatttgggt
     2161 gaagagtact ggggctacgc gaaaaataaa ctctggtcgc cgtttggcta tccggttcac
     2221 catgccaatg aagcgtcagc gcaggtcggt tccattgtta actgtatgtt caaccgtgac
     2281 tgcatgacgc atacccatat caactttatt ggttccggct tgccattgaa actgcaacgt
     2341 gaagtggcga aagaactttt tggttctgaa gatgcttacg atgaaaccaa aaactacacg
     2401 ccaatcaatg acgcaaaaat taaatatgct aagtggtcgc tgttgcgggt ctgtttgcat
     2461 aacgccgtca ccctgtgcaa ctgggtctgg ccaatgaccg tttcgccgct gaaaagccgt
     2521 aattaccggg gcgatctggc gcttgaagcc aaattcttca aagcgatcac tggcgaagag
     2581 atgactcagg aaaaattaga tttagctgca gagcgtatct ttacattgca tcgtgcctac
     2641 acggtgaaac tgatgcaaac caaagatatg cgtaatgaac acgatcttat ctgttcctgg
     2701 gtattcgaca aggatccgca gatcccggtc tttactgaag gtactgacaa aatggatcgt
     2761 gatgatatgc atgcctcgct cacgatgttc tacaaagaaa tgggctggga cccacagctt
     2821 ggttgtccga cccgcgaaac attgcagcgt ctggggctgg aagatattgc cgccgatctg
     2881 gcagcacaca atctactgcc tgcgtaagga atgggtaaga tgaaccatca ggatgaattg
     2941 ccgttagcga aagtcagcga agttgacgag gcgaagcggc aatggttgca ggggatgcgt
     3001 caccccgttg atacggtaac cgaaccagaa ccggctgaaa tcctggcaga gttcattcgc
     3061 cagcattctg tggctgggca gctggtggcg cgcgccgtgt ttctctcgcc accctattca
     3121 gttgcagaag aggaactgtc agttttgctg gaaaacatca agcagaacgg agactacgcg
     3181 gatatcgctt gtatgacggg aagtcaggat gattactatt actcaacgca ggccatgagt
     3241 gaaaactacg cggcgatgtc tttgcaagtt gtggagcagg acatttgtcg cgccattgca
     3301 cacgccgttc gttttgagtg ccagacgtat cctcgtcctt acaaagtcgc catgctgatg
     3361 caagcgccat attacttcca ggaagcgcag attgaagccg cgatcgccgc aatggatgtt
     3421 gcacctgaat atgccgatat acggcaggta gagtcatcga cggcggtgtt gtacttgttt
     3481 agcgagcgat ttatgaccta tggcaaggct tatggcttat gcgaatggtt cgaggtagag
     3541 cagtttcaga acccctgaaa tttcagggag atgtcatgga tagggtggac agttgcagcc
     3601 actgcattag gggataatca gatgtccttc actcgacgca aatttgttct ggggatggga
     3661 acagtaatct tttttactgg ttctgcgtct tctctgttag cgaacacgag gcaagaaaag
     3721 gaagttcggt atgccatgat tcatgacgag tcacggtgca atggctgtaa tatttgcgcc
     3781 cgcgcatgcc gtaaaactaa ccacgccccc gctcagggaa gccgtttatc gatagcacac
     3841 attccagtca ccgataacga caacgaaacg cagtatcact tttttcgcca gtcatgtcag
     3901 cactgcgaag atgctccgtg catcgacgta tgcccgacag gggcgtcgtg gcgtgatgag
     3961 caggggatcg tgcgggtaga aaaatcgcag tgcatcggct gcagttattg cattggcgct
     4021 tgtccgtacc aggttcgcta ccttaaccct gtcactaaag tggcagataa atgtgatttc
     4081 tgcgctgagt cccgactggc aaaaggcttt ccgcccattt gcgtcagcgc ttgcccggaa
     4141 cacgcattga tctttggtcg cgaagatagc cctgaaattc aggcgtggtt gcaggataat
     4201 aaatactatc aatatcagct acctggtgcc gggaaaccgc atctgtatcg ccggttcggt
     4261 caacatttga ttaaaaagga aaatgtatga acccgtcgca acatgctgaa cagtttcaga
     4321 gccagttagc gaactatgtg ccacagttca ctcccgaatt ttggccggtg tggttgatca
     4381 ttgccggagt attgctggtt gggatgtggt tggtgctggg gctgcacgcc ttgcttcgtg
     4441 ctcgtggcgt gaagaaatca gccaccgatc atggtgagag gatttatctt tacagcaaag
     4501 cggtcagatt atggcactgg tcgaatgcgt tactctttgt attgttgctg gcaagtgggc
     4561 tgataaatca ctttgcgctg gtgggcgcaa ctgcggttaa aagtctggtt gcggtgcatg
     4621 aagtttgcgg atttttgtta ctggcatgct ggctcggctt tgttctgatc aatggcgttg
     4681 ggggtaatgg ccaccactat cgcattcgtc gtcagggttg gctggagcgg gcggcaaaac
     4741 aaacgcgatt ttatttgttt ggcattatgc agggggaaga acatcctttc ccggcaacaa
     4801 cccagtctaa atttaatccc ttacagcagg ttgcctatgt tggtgtcatg tatggattgc
     4861 tgccgttgtt actattgacg gggctgctgt gtctctatcc gcaagccgtg ggagatgtgt
     4921 ttcctggcgt aagatactgg ttattgcagg cgcattttgc tctggcattt ataagcctct
     4981 tttttatctt cggtcatctt tatctttgca ccacggggcg tacgccacac gaaaccttta
     5041 aaagcatggt cgatggctat caccggcact aacgaatgat cattacacga gcagacctgc
     5101 gagaatggcg tatcggagcg gtgatgtatc gctggttcct gcgccatttc ccacgcggcg
     5161 gtagttatgc cgatattcat catgcactga ttgaggaagg atatacggac tgggcggaga
     5221 gtctggttga gtatgcgtgg aaaaaatggc tggcggatga aaacttcgcg catcaggaag
     5281 tttcctctat gcaaaaactg gccacagacc ccggagagag agcattttgc agtcagtttg
     5341 cccgcagcga cgatcatgct cgcatcggct gttgtgagga taacgccaga atcgcaaccg
     5401 ctgggtatgc tgcacaaatt gccagtatgg gttatagcgt gcggattgga agtgtcggct
     5461 ttaacagtca cattggcagc tccggtgagc gcgcacgggt tgctgtaacc ggcaattctt
     5521 cgcggataag cagcgctgga gatagcagtc ggattgcgaa taccggaatg cgagtacgcg
     5581 tttgtacatt gggtaaacgg tgtcatgtcg ccagtaatgg tgacctggtg caaatcgcca
     5641 gctttggcgc aaacgccaga attgccaaca gcggcgacaa tgtacacatc attgccagcg
     5701 gtgaaaattc aacggtagtg agcacgggcg tggtcgactc aattattctc gggccagggg
     5761 gcagcgccgc actggcatat catgacggcg aaagagttcg cttcgccgtc gccattgaag
     5821 gtgaaaacaa tattcgagcc ggagtccgct atcgtcttaa tgaacagcat cagtttgttg
     5881 agtgctgata gctttacctt taatcaaacg aaagacgccg acgagcacgg gatgcaggct
     5941 ttacgaccgc ccgagccatc gcctcaccaa tttctgcaca tgccgtaagt ccctgaacga
     6001 aaggctggtc gtgcatcaac cagggcaacg cgccgaacgc tacgcgccca cgaatatctt
     6061 cgggttgctg taacgtataa tcttcaccaa catcagggat ttcatcccct gttttctgta
     6121 attgttcgcg tagtccaggg aaaggaatat ctttcacttt aagcggacgc tgcccgcggg
     6181 catcaataaa aacgtcaaac gagtagctgt tgtcttccgt tttcaggacg gtgcgcgact
     6241 cgttaatttc cattttgtag tcttcaccga gagcgagaat atgaatgatt cccgcttcac
     6301 gtaaggcaag taggcgacga atagactctg aagggattgc cgcataatta tcgatgaaaa
     6361 cccgggcaag gcctttactg aaccgtttat ggtcgtgttc attcagatgt ggaacaattt
     6421 cctgtacggc ttcatgcagg cgcagaatga cataacgcca gggaacagta tgtttttctc
     6481 gtttattgcg ttcgacttcc tggagatttt tttctgccca gtcaaattgg tcgcgttgtt
     6541 tgcgctcggc aaaccaggct tgagcaaagg aatcgacatt caggctctct aaggctatgc
     6601 gttgactcca gtctggatca gcaaacttga tttcctctac tatcaatcta aatacccgat
     6661 ccaaaaggcc atattcgcct ttttgaatct cagcatttaa tgcctgatca gtgacgatgt
     6721 gtaagggctc gtagggaata gggcaataga aatcggcttc gggtaaaata cccgtgcgcg
     6781 acattaacgt aatatttagc ttttcacttg cgttatcgcg gtgaaaaacg acgtgttgtt
     6841 tatcatcttc aatgaacgaa ccatgctgaa tagccactgc cattgccgca tccagtccgc
     6901 tcaaggatgt tcccataata cccacgttac acgcatcgac ctttgcttcc atcaagcctg
     6961 accacgggct tggaaaatac gttcgggttg cttcttcttc atcaggccag acgtgacccg
     7021 tggcgatcac cgctaaatca aacgtctcgc tgggtaaatc ctgatttgta gcgagcatga
     7081 cgccagcatt tgtaatttgc agatcggtaa cctggcatga ttcataaaca gccactgcaa
     7141 atttttgctt tcgtgcctgg tctactaatc gtaaaaattg atcgcggaaa tattcgccca
     7201 gcagaattcg cggtaaaaac tgacgatcgt gcaaggtttc ttttttaacg ccataacgct
     7261 ggagatgact ggcttcttgc ttttgtagcc attcgagata cgtacaataa atcggcggta
     7321 tttcaatact ggcaatattt gccagcatca tttttgagtt ttcctcatca ctgtatggca
     7381 tcccgacacc ggcctcgtca gcctgctcga agatagaaat agaaagtgga gtttgttgct
     7441 gtagaagcga gaataaggtg tagatccccg taggcccggc acccacaatt gcaatttttt
     7501 tcattgttac ttacctgtac gcattctttc gaatttagtg ttatcagata agtttggttg
     7561 agatttttag aatttgagaa atttcagaca gggatgaaaa gggcgctaac cgcccttgat
     7621 taaattctgt caggcgagtt ttgcctgatt gatttgactc agtggctcat tgacatcgaa
     7681 aactttggcg acaacttcct caacgccgag ttttttcagt tgggcagtgt gtttttccag
     7741 ataggcaagg gcgctttttt catcagtgaa aaggtagatc ccaccggctt cgtggttctt
     7801 ttcactttct gtccatacct tccacagaaa accaggttcc tgattaatcg actctgcaag
     7861 tggtttaagc tgctcagcca ttgcgtcgcc aaatgggcca ttaaaagcaa aatgaagttg
     7921 taacaaagtt gccatagcga ttctccttct gataacgcat acgaagacag tgtaaatctt
     7981 ttaacgtatt ggatacagag aagaagcaga gggaaaaagg agaagaccag attaacaaaa
     8041 tctggtgcgt ccgagtggac tcgaaccacc gacccccacc atgtcaaggt ggtgctctaa
     8101 ccaactgagc tacggacgca ggatggtgcg ttcaattgga ctcgaaccaa cgacccccac
     8161 catgtcaagg tggtgctcta accaactgag ctatgaacgc aacgttgtag gtgacaacgg
     8221 ggacgaatat tagcggcaga gtgggaaggt ggcaagaggc aaaacgtaat tttctgcgct
     8281 atttcgaccg tttgcagagc ttttaagcaa attggctata ttttgttgat ttgcaagggc
     8341 gatttttatt caggatcgca tttacatctg atacaaccca ataattcgtg tatcagagtt
     8401 taaacagagt tgtttgccac catctcaaat ctgtcgtgga taaatcctcc gtagccttta
     8461 cacttaggta gcaataaagg cacacagatg cagcaaagga ataaatcatg ggatctgatg
     8521 cgaaaaactt gatgagcgac gggaatgtgc aaattgttaa gaccggcgag gtcattggcg
     8581 cgacgcaact tactgaaggc gagttgattg ttgaagctgg cggaagagcc gaaaataccg
     8641 tggtcacggg ggctggctgg ttgaaagtgg caaccggtgg gatcgccaaa tgcacacagt
     8701 acggtaacaa tggcacgcta tcggtcagcg acggtgccat tgccacagat attgttcagt
     8761 ccgagggagg cgcaattagt ctctctacgc tcgctacggt taatggccgc catcccgaag
     8821 gtgaattcag cgttgatcag ggttatgcct gcggtttgtt gctggaaaat ggcggtaacc
     8881 tgcgtgtact ggaaggacat cgcgcggaaa aaatcattct cgatcaagag ggcggcctgt
     8941 tggttaatgg gacaacctca gcggtcgtgg tagatgaagg tggtgaattg ttggtgtatc
     9001 caggtgggga agccagcaat tgtgagatta atcagggcgg cgtttttatg ctggcgggga
     9061 aagccaatga tacgttgctt gctgatggca ccatgaataa tctcggtggt gaagactctg
     9121 acactattgt tgagaatgga gccatctatc gtctggggac ggatggtctt cagctctaca
     9181 gttccggtaa gacgcaaaac ctgtccgtta atgtgggtgg tcgggctgaa gtgcatgccg
     9241 gtacgctgga aaatgcggta atacaaggtg gaacagtgat cctgttgtca cccaccagcg
     9301 cggacgaaaa ttttgtcgta gaggaagatc gcgcaccggt tgaactgacc gggagtgttg
     9361 cattactgga cggcgcttca atgattattg gctatggcgc agatctgcaa caatcaacga
     9421 ttactgtaca gcagggcggt gtattgattc tcgacggcag tacggtaaaa ggtgacagtg
     9481 tcactttcag tattggtaac atcaatctga atggcggaaa actgtggctg atcactggtg
     9541 cggcaacgca tgtgcagctg aaagtgaaac gcctgcgcgg agagggagcg atttgcctgc
     9601 aaaccagtgc gaaagaaatt tcacctgact tcatcaatgt gaaaggggaa gttaacgggg
     9661 atatacgcgt tcagataaca gatgccagtc ggcaaactct gtgtaacgca ctgaaactac
     9721 agccagacga agacgggatt ggcgcaacgc tccagcctgc gtaaaaacac aggcgcaccc
     9781 cgtgaaggtt gcgccttgtc tttattagcg ggatgctcgt tgcagaatga tggctgacgg
     9841 cagacgttgc aggtaccgca tacgcagcat catcataatg gctgccgacg tcaggccaat
     9901 aataaagcct atccagaagc ctgctggccc cataggttca acgaccagat cggtcagagc
     9961 cagaatatag ccgcttggca agcccagcac ccagtaagcc gtaaaggtga tatagaaaat
    10021 ggaacgcgta tctttataac cacgcaaaat cccactgcca atcacctgga ttgagtcaga
    10081 aatctgatat accgccgcca gcaacatcaa atgcgccgcc agcgttacaa cctcgggatt
    10141 gtcgttgtac aacagggcga tttgctcccg cagtgaaacc gtgaatatgg ccgtcagggt
    10201 tgccatacag acacccacca taagcccggt ccgcgcagcg gtttgcgcat ccagcgttga
    10261 gccctgaccc agacgataac ctacgcggat agttaccgct gccgccagcg acattggaag
    10321 cacgaacatt agtgaactaa agttcagggc aatctggtgt cctgcgacat caacaatacc
    10381 gagcggagac actaacagag ccacgacggc aaacagtgtc acttcaaaga atagtgccag
    10441 cgcaatcggc aaaccgagtt gaatcagtcg tttcataacc gcaggatcgg gtttcgcggt
    10501 gcctttttcg ttacgaatat cgcgcatcga gcgggcgcgt ttaatgtaag aaaccatggc
    10561 aaggaacatg acccaataca ccgccgcagt agccacgcca caaccaacgc caccgagctc
    10621 aggcataccg aaatgaccat aaataaagat atagttcacc ggaatgttca ccagcaggcc
    10681 gataaagccc attaccatac ccggcttggt ttttgccaga ccttcacatt ggttacgggc
    10741 aacctggaag aaaagatagc ccggcgcgcc ccacagcaac gcacgcagat aacccacggc
    10801 tttgtccgcc agagccggat cgatgttttc catggagcgg ataatgtaac ctgcattcca
    10861 cagcaccagc ataatgagaa cggaaacaaa acccgccagc cagaagccct gtcgcacctg
    10921 atgcgcaatg cgctcacgtc gaccggaacc atttaattgc gcgataaccg gcgttaatgc
    10981 cagcagcagt ccgtgaccaa agaggatcgc cggaagccag atagaagtac cgatagcgac
    11041 cgccgccatg tcggtggcac tatagccgcc cgccatcacg gtatcgacaa aacccatcgc
    11101 agtttgggcg atttgcgcga gaatcaccgg gattgctaat gccaataaca gacgcgcttc
    11161 actgatatac ttctgcacgt gaacaccttt tatttgtagt tatatgagag actaaaaagc
    11221 cgcctgaata ggcagcaaaa agaagaagca ggggaaattc agtctattgt agcgaggatt
    11281 tattaattct ccagtgaaaa aatagccatt cttacccctt cactggtaag ggcgttttcc
    11341 acctgctatt gtgctgaaca gaatgtctta actgatttca ggagttgtaa gtatgtttac
    11401 ggggattgta cagggcaccg caaaactggt gtcgattgac gagaaaccaa attttcgcac
    11461 acatgtggtg gagttacccg accacatgct ggacggtctg gaaaccggtg cctccgtggc
    11521 gcataacggt tgctgcctga ccgtgacgga aattaacggc aaccatgtca gttttgacct
    11581 gatgaaagaa acgttgcgca ttaccaatct tggcgattta aaagtggggg attgggtaaa
    11641 cgttgagcgt gcggcgaaat tcagtgatga aattggcgga cacttaatgt caggtcatat
    11701 tatgaccacc gctgaagtgg cgaaaatatt aacctcggaa aataatcgcc agatctggtt
    11761 taaagtccag gatagtcagt tgatgaaata tatcctgtac aaaggattta ttggcatcga
    11821 cggtattagc ctgaccgtcg gcgaagtcac gccaacgcgt ttttgcgtcc atttaattcc
    11881 ggaaactctg gaacgcacga ctcttgggaa gaaaaaactt ggcgcacgcg tcaacattga
    11941 aatcgatcca caaactcagg cagtggtaga tacggtagaa cgtgtgctgg cggcacgaga
    12001 aaatgccatg aatcaatcag gtacagaagc ctgatctgaa atcgcccccg gagagggggc
    12061 gatagaatag cctttagcga gcgactcgaa ggccgttttc aacaccgcgt gagaacacca
    12121 cctgccagag ctgaatatca cgggcgcgga aagcacctgc gcaggcattc agataatagg
    12181 taaacatccg tttaaagcgt tcactgtagt tatccgcaat ttctggccat gcggcgacga
    12241 atcgttcata ccacgccatc aacgtagtat cgtaatcagc accgaagtta tgccagtctt
    12301 ccatcacaaa gtggggttcg ctggactgag caatctggcg tacagagggc aggcaaccgt
    12361 tcgggaaaat atatttatta atccagggat caacattcag atcggttttt ttcgaaccga
    12421 tagtatggag caggaatatg ccttccggtt tcaaattacg atccaccacc gtaaaatagg
    12481 tatcgtaatt tttcggtccg acgtgctcga acatccccac agaaacaata cgatcaaact
    12541 ggtcgttcag gtcacgataa tcttgcagca aaatggtgac atccaggcct tcacagcgtt
    12601 cctgagccat tttttgctgt tcggcagaaa tggtgacgcc caccacgctt acgtcataat
    12661 tagatgccat gtagtgtgcc agtccgcccc agccgcagcc aatatccagt acgcgcatcc
    12721 caggttttaa ctgcaatttt tcacaaatca ttttgagctt cgcctgctgg gcagattcca
    12781 gattatcggc atctttccag taagcgcagg aatattgcat gaagggatca agcatgcggc
    12841 tgaacaagtc attacccaaa tcgtaatgct ctttgccgac tatccaggca cgttttttac
    12901 tctgcagatt gaagagacgg gcgccggcaa tacgcagcgt gtctttgaaa tgatggggga
    12961 gttggttctc gagacctgcg cgtaagactt tgctaaaaaa catatccagt cggtcacatt
    13021 cccaccagcc atccatataa ctttcgccta accccaaaga gccttcttgc agaacgcgtt
    13081 taaaaaaatc ggggtttttc acacgaatat ccgccggggc agaaccgtta atggctatac
    13141 cggcacggct aagtaattcg ttggcgatac ggtaccagtt gtcatccggt acactgactt
    13201 cttctataca cgatgaactc atagtttctc catcactggt cggtgatcag aaccttaaaa
    13261 cagcgtagac gctttttggg ctttgtgaga aatctcgcag aaaaaaccgc gagtctataa
    13321 tccgacgtag aacagaggga aaggaggaac ccttgccgaa atgaccatcc atagtgagtc
    13381 gggaacgatc ctgtccccgt aaaaacataa tatttatcgt agctttaaca actcgaaatt
    13441 agtataggcc gctaatttca atcatacaac aaaattttgt tagcagccta agtataagta
    13501 tatcgatata gatcagtgtg attcgctatg agcgacttcg gcattgccat gattctggca
    13561 gccaacttct tcacaacgtt gcatcatgta acccagcgcg accagcacta ctgttgataa
    13621 catcacgctg gtggtggtga gcaatggcgt gctgatactg atcagccagg aaactaccag
    13681 acttgcgagg aagcacagac ccagttgcag agtgttctgc aacgctgcgg cgcgaccagt
    13741 tgcgtgtggg aagggacgca gagcctgggc gacaacaata gggtagatcg cgccattagc
    13801 aatcgccatc acacagaatg ggatcaggat ttcgaccagc gacacatggc taatgaagcc
    13861 cgcagcccag gtcgcaatga cgctgacagc aaacagcacc agcaaccacg gtaataactg
    13921 cttgccttgc catttctgca gcgcggcgcg acagccataa ccaccaatca gaaacgcgat
    13981 agtttgcggg acataactta aaccaataac tgccgggctg tagcccattt cactaaggat
    14041 gaacggtgaa ccggtcagcc atgcaaaaaa actggctgaa caggccgcgt atatcagcac
    14101 attgccgcga taggttttag aacgtagcag gtcggtaaag gtcagaccat cctgactatt
    14161 gttacgggcc ttcgtcgtgg gtttgagcca gaaaataggc agaatcagca ccacggtaat
    14221 ggcaaaaagg gtggcgaaaa tcgcctgcca ggaaaaatgg accagcagcc agcttcctaa
    14281 cagaggagcc agggccggag atagacccac cagcggcatg atggtcgcaa aaattcggtt
    14341 aactttctgt gaaggataat aatccgtcac taacgcttgc cagataaccg ccgcggcgca
    14401 gacacccaca gcctgtacaa aacgcaatac cagcagcata gcggcgtttt ctacccacag
    14461 catccccaga ctacctaacg caaaaattgt caggccgatt aataataccg gtttacgacc
    14521 ataacgatcg gagagcggcc cccacagaag ctgggctgcg gcaaaaccgg caaggaacag
    14581 actaaggctg gcactgacag cagacgcagg cgtttgcagg tcggcctgta tggcggcgaa
    14641 agcaggcaga tacatatcgg ttgccagaaa accgagtacg ctcaaacccg ccagccagac
    14701 taaaaatctt ttcccaggtt gcatgtatat tctctaaatc tctcaattaa acaccgccgc
    14761 aaagtgtagg gagtgaaatt agtgttgtga aacggtaata tttgatgact ggtttcaaaa
    14821 attttgcagg cagactatgt ggtcagaata ttcactcgaa gttgttgatg cggtagcgcg
    14881 taatggtagt tttagcgctg cggcacagga gctgcatcgc gttccttctg cggtcagcta
    14941 taccgtgcgt cagctggaag agtggctggc ggtgccgctg tttgaacgac ggcaccgtga
    15001 tgtggagctg accgctgccg gagcgtggtt tctcaaagaa gggcgctctg ttgtcaaaaa
    15061 aatgcagatc acccgccagc aatgtcagca gatagcgaac ggctggcgcg gtcagttagc
    15121 tatcgcagtg gataatattg tcaggccaga acgtacacgg cagatgatcg ttgattttta
    15181 tcgccatttt gatgatgtcg aacttcttgt ctttcaggaa gtgttcaacg gtgtctggga
    15241 tgcgctttcc gacgggcgcg tggaactggc gattggcgca acacgcgcga ttccggtagg
    15301 cggtcgttat gccttccggg atatggggat gctaagctgg agttgcgttg ttgccagcca
    15361 tcacccgctg gcgttgatgg atggcccgtt cagcgatgat acgttgcgca actggccgtc
    15421 gttggtgcgc gaagacacct cgcgaacgct acctaagcgt attacctggt tgctggataa
    15481 ccaaaaaaga gttgtcgtgc ctgactggga atcatcggca acctgtatct cggcaggatt
    15541 atgcataggg atggtgccaa cacatttcgc caaaccgtgg cttaatgagg ggaagtgggt
    15601 agcattagaa ctggaaaacc cctttccgga ttcggcatgt tgtctgacat ggcagcaaaa
    15661 tgatatgtcg cctgcgttaa cctggttgct tgagtatctc ggcgatagtg agacgctgaa
    15721 taaggagtgg ctgcgggagc cggaagagac tcccgcaacg ggtgattaac gacgatagtc
    15781 gcggaacggg ccgtcagcca cggagcggcg ttcaatcaag cgcggatgca cttcaatgga
    15841 ctgcggttct tcacgtttgt tgactatacg atccaacaac atgttgaacg ctgtttcacc
    15901 caacgaatct tttggctgat ggattgttgt cagcgccggc gtaaaatagc gggcgttgcg
    15961 cacgttatca taaccgatca gcgaaacatc ctgcgggacg cgcagcccca tttcatcagc
    16021 agcacaaagt gcgcccattg ccatgatatc gccaccacag aagacggcag tagggcgatg
    16081 cggctgcgac aggatttgct gcatggcgcg ataaccggat tcaggttcaa agtcaccctg
    16141 cacaatccag ctttccggca ccttgatcat tgcttcttcc atcgccttca taaaaccggc
    16201 aaggcggcct gcgccagtgt tacgttccag cgggccgggg ataacgccga tttcgcggtg
    16261 accgcgttca atcagataac gcccggccat gtagccgcct tcgaacgcgt tatcaatgac
    16321 cgcatcggtg aagtcagctt ttgcttcacc ccagtccatc accaccattg ggatatggcg
    16381 atactcttcc agcatcgcca gcaacggctc tgggtactca gaacacatca ccagcagacc
    16441 atcgacgcgt ttttgcgcca tcatcgacag ataagcccgc tgtttctcaa gattgttcca
    16501 cgcattaccc agaatcaggg tgtaaccttt ctggaagcaa tttttttcaa ctgcttcaat
    16561 gatctcggca aaataggccg cttcgctgct ggtcgctagc aaaccgatag acttggtgtg
    16621 gttaaccttc aggctacgcg ccaccgcgct aggggagtag tgtaattctt tgatcgctgc
    16681 ccacacggcg ttgcgcgttt cttcagcgac gaaacgtgtt ttgttgatca cgtgtgacac
    16741 agttgtagtg gaaacgtttg ctcgtttcgc tacatctttt attgttgcca ttccatttca
    16801 ctccagaccc taacttcagc tcctgcaaaa tcgcaaggta aacgtttgcc tttacacacc
    16861 ttacgcacaa caaaagtgtt gcggtacgcc ggaaaaacga cacggagcgt caggaagggg
    16921 tcaatggcca gtacgcaaat agtgtaagcg tggaattttg gccgatcttg acgaaaaggg
    16981 gaagtggtaa aaacacttat cattataaat gacgggagat tttttcatca cagtgtgtaa
    17041 aaatgcgtaa tatcactttt tgtgtggtaa taaaaaagga gaaaacttga tgagcaccga
    17101 ccttaaattt tcactggtaa caacgattat cgtcctcggt ttgatcgtgg ccgtgggttt
    17161 gactgccgcg ctgcactgat ttcctctcga gggagcgcgt tctccctcgc atctccttcc
    17221 tgtttgttag caaaatggca aaagtgtttt ctctttttgt tattttcatt tttttgtgat
    17281 tgctgtcatg ctttcgccac tttaatgaat atcgcgtaat gacgcgaaga ggtggagata
    17341 aggcatgaaa attaactatc cgttgctggc gctggcgatt ggcgcgtttg gtatcgggac
    17401 aacggagttc tcgccaatgg gcttgttgcc cgtcattgcg cgcggtgtgg atgtctcgat
    17461 tcccgctgcc ggaatgttaa tcagtgccta tgcagttggc gtaatggttg gcgcgccgct
    17521 gatgacgctt ctactttctc atcgtgcccg ccgcagtgcg ttgattttcc tgatggcaat
    17581 tttcacgctc ggcaacgtac tttccgccat cgcgccggat tatatgaccc tgatgctttc
    17641 acgcattttg accagcctga atcacggagc attttttggt ttgggttcag tcgtggccgc
    17701 aagcgtggtg ccaaaacata aacaggccag cgcagttgcc actatgttta tggggttaac
    17761 cctggcaaat atcggtggcg tgccggcggc gacctggttg ggtgaaacca tcggctggcg
    17821 gatgtcattt ctggcaacgg cggggctggg agtgatttca atggtaagtc tgttcttctc
    17881 attacctaaa ggtggtgcag gggcacgacc tgaagtgaaa aaagagctgg cggtattaat
    17941 gcgtccgcag gtgctgtctg cattgctgac gacggtactg ggagctggtg caatgtttac
    18001 tctctacacc tatatctctc cggtactgca aagtattacc cacgcaacac cggtgttcgt
    18061 cacggcaatg ctggtgctga ttggtgtcgg attctctatc ggtaactatc tcggcggcaa
    18121 actggcagat cgttcagtta acggcacgtt gaaaggcttt ttgttgctgc tgatggtgat
    18181 tatgctggca atcccgttcc tggcccgcaa taagttcggc gcagctatta gcatggcggt
    18241 gtggggcgct gcaacctttg cggtcgtacc gccgttacag atgcgcgtga tgcgtgtcgc
    18301 cagtgaagcg ccaggtctgt cttcatcagt caatattggt gcctttaatc ttggaaatgc
    18361 gctgggagca gctgctggtg gtgcggtaat ttccgctggg ctgggataca gctttgtgcc
    18421 ggtgatgggg gcgattgtcg cgggactggc attattgctg gtgtttatgt cagccagaaa
    18481 acaacctgaa acagtttgcg ttgctaacag ctaattaaac aaaaacgcag aaggataacc
    18541 ttctgcgtta cagttgctga caacgtgcga tttatcttgc cgaatgcgct gttaacgcct
    18601 catccgcgtt ggcatggtaa gcgaagcgca tcaggcaata ctgcatttgc catcagttat
    18661 tatgcagcga gatttttcgc tacgaattcc cagttcacca gcgcccagaa gtgctcaaga
    18721 tagccaggac gtgcattgcg atagtcgatg taatatgcgt gttcccagac atcaacggtt
    18781 agcagcggag tcgcatcggt ggtcagcgga gtacccgcgt tagaggttga aacgatagcc
    18841 agtttgccat cgctgttttt caccagccag gtccagccag aaccaaagtt tttgatcgct
    18901 gcatcagtaa actgagcttt gaaatcggca aagctgccaa aagatgcggc gatagcttca
    18961 gcgacttttc cagtcggttc gccaccggcg ttcggtgcca ggcagttcca gtagaaagta
    19021 tggttccaga cctgagctgc gttgttgaat acgccacctt cagagctgcg aataatctct
    19081 tccagtgatt taccttcaaa cgcggtacct ttaatcaggt tgttcaggtt agtgacataa
    19141 gtctgatgat gcttgccgta gtgatactcg atagtttccg cagaaatgtg cggtgccaga
    19201 gcatctttag catatggtag tgcaggtaat tcgaatgaca ttgctactct cctttattat
    19261 taatttgcat acgtacaata gccttattgt gcgtatggat agggtagcaa attaaaaggt
    19321 gagacaaaag atgaacttac cctgttgccg taacaacagg gtaaagtttt agcgaagtgt
    19381 ttttggggtc attacccggc gagcgccaac atagtggcgc tgccagtagt cttcactgag
    19441 agaagtgatt tgaatttcct gacctgtgcg cggtgactga ataaatttgc cgttgccgac
    19501 atacacgccg acatgatcgg ctgtaccgcg tccctgagta cggaaaaaga ccaggtcgcc
    19561 gtttttcagt tcactacgtt cgattggcgc tgcatcgcgc aggtgataca tttcattcgc
    19621 cgtacgcgga atacgaattt tcaccaaatc tttataagcg taataaacca ggccgctgca
    19681 atcaaaaccg gtacgcggtg agctgccacc ccaacgatat ggcttaccaa tttgctgcat
    19741 cagtttattc attgccactt ttgtggcttt ttgcaccttc gctttgtgtg cgtcggcaag
    19801 ggttcctgac gctgcatttt tgactttcac acaatgcgat ttatagccct tacgtttggt
    19861 acacttttcg gtaaggctgg ctgaggccgt tttattgacg gtatgtttgc tgcgagtttt
    19921 ggcgttttta gaagcggtaa tggaagattt tttaacggaa gaagcggttt tgcttttggt
    19981 cgtactttta ctggcggctt ttttcgctgt tttctgggtc tttttggtgg ttgccgtgct
    20041 tttcttttta tctgcttttt tggtgatatg agtggtagca gaactctccc tggcttgctt
    20101 tgaagcatgg gccataggcg ttaaaggcag ggtggtaaaa agtaaagcac agagcgtgat
    20161 cgaaatacgg tttatccgcg ccactgagcg tccctctgtt aaggcaggtc tatcatgcct
    20221 gcgtctgatt tacaaaactt gatgattcta atgcatagaa gaagagaaag ttaatagctt
    20281 ttattgttaa aaaatacgtt tcgttggcga gcgcaaaaaa atgcagcagt gaaagctaaa
    20341 aatggtcata aatacgccag tttgccgtgc atcagggaag aaacgaatat ttgaaatgca
    20401 acaaatagag caaagggata aaatagcaaa agcgtgatga aaatgttatt ttccgatgcc
    20461 gcactgaccc gctacaatgt cagattactg ccgtaaagaa gtttgaggaa gcaagacaat
    20521 gagcaccact atcgaaaaaa tccaacgcca gattgctgaa aacccgatcc tgctgtacat
    20581 gaaaggttca ccgaaactgc cgagctgcgg tttctctgcc caggcagtcc aggcgcttgc
    20641 cgcatgtggc gaacgttttg cctatgttga tattctgcag aatccggaca ttcgtgcgga
    20701 gctgccgaaa tatgctaact ggccgacctt cccgcaactg tgggttgacg gcgagctggt
    20761 aggcggttgt gatatcgtga tcgaaatgta tcagcgtggc gaactgcagc agctgatcaa
    20821 agaaactgcc gctaaataca agtctgaaga gccggacgcg gaataatccg tcctgcaata
    20881 ctgacggatg cgaacgggca tccgtcagtc ctttctctat ccccaatcca gcccttgtgg
    20941 cgaacttgaa aatcccgctt cacgcagcag cgtaaacatc ggcgtttgcc agaccggtag
    21001 atcattcact tctgttagcg taaagcgcag ccgtggttcg cgacgcagtg cggtagtcag
    21061 cgcgtggaaa acctctgggg cgagtaattc ctctttttcc tgccagacca gcattttttt
    21121 gccaccttgc gccagataga gtaacaattt gccgccagaa acgaccacca gcgcacccgc
    21181 ccgacgcgtt ggtaccagcg ttgccggatg tgcaggccag ggcagaagat ttccccacac
    21241 attagccgga tcgttggcgg agagcgccac tggtgtatag tggcgcgttt gcgcggcttg
    21301 tgtcgccagg tcgcgcaagc ggtcaatagt cagacgttca gcgaattgcg cgccacccag
    21361 accttctaca aaacgacctc gcataattcg cccggaatct tccatacttc gacaaagcgt
    21421 ttgcatcgat ggaaacccgc cagggatatt ttcggctatc accgcctgac gactgatgat
    21481 gccgtagcgg tcgagcatat tttccgccag cgccagcatc ctttcggtat cgtttagtgg
    21541 ctccacctgc aataacgacc agcgtccagc cagatttggt gtgttgtaag atacccgcgg
    21601 cgagacgggt tgtgcataga caggacgccc acgacgagcc cggtgactgc ggcgagttga
    21661 ggtgcgtgcg ttggaactgc tgcgggtgag ggcgcgtaac ggtgcccaaa tatcgctggt
    21721 gatgacgcct tgccagacca gcgcccataa cgcctcttgc agtgccgagg gatcaaccga
    21781 ttcgccgatt ttgtcgcgta tccgttggct gatttgttgt gcaaaccagg ctcctccgtc
    21841 agccagaaca gcgactatcg cttgttgcag cgccgaacga ttcgcctgat ccgcttcggc
    21901 gggagtgaac gattctgcag catattcctg tagatgcagt gccaccaggc cgtcatcttc
    21961 acccagtttt ttttgccccg accagataac cgcaccggtt gccagtaatt catcgagcac
    22021 ttctggcgaa tagtcgtgta cgcgggcagg caagatctgg ctttcccaga gtgaggcggg
    22081 taagccgact ccggcaagct gttcgatcac ccgcatcacg ccatccaccc cctcataaac
    22141 gcctggcgat gttgaggcaa agagcgccgg gctaccatcg gtggcgggta atacgccctg
    22201 acgttccagc agcaatcgcg cataggtcgt ggctgcaacg ggacgcgtcg cttctctggc
    22261 ggcttgcagc gagcgcaaac gcagacgacg aaatacttca tcgctcaccc agatgtcttg
    22321 ttgcagattc atcaccagac cctgttcgcg cagttgctga agctgctctt cgacaatggc
    22381 aataccgaga ctaaactcat gagccagttg ttcagccgtg accagagcat gagcccggag
    22441 atagcgtaga aagaggtcgc gaagcgggta actgactcta tgcaaataaa tctctggcag
    22501 tgactctggt aatcgtacgc cgagggcatc gcgcagcctg gcggcatcat ccatacaggc
    22561 aagacgctcc tgtccactaa tcatcgtcgg gaagatgcgt tttactgtaa gaagattttc
    22621 cagatacgag gcaatctctt cactgctgcc tgtatgccgt tgcgccaggt cttcaacggt
    22681 cattggcccc agttcgcgca gcaggtcgaa caatccttct tcacctttcg ctcttctgcc
    22741 aggagccagt cgttgcaact cttcttccac ctagcgaatg acctgcgggt cgagtaattc
    22801 ccccggatcg acctgtccga gtagattgcg cagtaactcg ctgtccagcg acagtacgga
    22861 tgcccggcgt tctgccagcg gggcgtcgct ctggtacata aattccgcga catagccgaa
    22921 caataaactt gtggcgaaag gcgagggcgt agtagtcgtt acatcggata tttgaatttc
    22981 gcctccgttc aggcgacgca tcaaacgttc cagtgcggga agatcataaa catcttgcag
    23041 acattcgcgt agggtttcga gaatggccgg aaaatccgga tatccctgag cgatttccag
    23101 caactgactg gcgcgcagcc gttgttgcca aagcggggtg cgatggcccg gcgtgcggcc
    23161 cggcattaat aatgcccgcg cggcgcattc gcgaaaacgg gcggcgaaaa gtgccgaact
    23221 gcctaccgcc tcgcggacaa tttgcagcaa cttttctggt tcaaacaaaa aaatcgcggc
    23281 gtcgggcaat ttaccatcag tgtcaggaat gcgcgcaaca atgccgtcat cactggcgac
    23341 cacagacgcg tcagcgcccc aaagcgcatg tattcgcccg gcaatcgcca gcgcccaggg
    23401 ttcatgcacc cgtcttccat agggagagtg caaaataata cgccaatcgc caatttcatc
    23461 acggcaccgt tcgaggacca gatgacgact ccccggaaca atacccgtcg cgttgcgctg
    23521 ctcgtcaatc aacccctgaa tattggcgat cgtattttct tcagccagcc acgggggaat
    23581 agtcccggaa aagaacgctc catccgccag caaatgaaga aaatcgccga tcatctcgcc
    23641 taattcagcc ggacgtccgt taccttcacc acgccagaag gggagccggg cagaacgacc
    23701 cggagcagga gtcacaatca cctgatcgcg ggtgatttgc tggatccgcc atgaggttgc
    23761 gccgagagtg ataatatcgt tcacccgcga ctcatatacc atctcctcat ccagttcacc
    23821 cacccgtcgc gaaccggcct gttcttcccc ttcgggtaat aacacgctat acatgccacg
    23881 atccggaatg gtgccgccgc tggtaaccgc caacagctga gcgccaggtc gggcggtcaa
    23941 tatcccggtc tccctgttcc agaccagttt ggggcgaaaa gcagaaaaat cgccagaggg
    24001 atagcgtccg gaaagcatat ccagcgtggc gtcaaaaaca cgtcttggca gatctttcca
    24061 cggtgcggca cggcgtacgc gggagtacca ttcgtctacc tgtaatgcat ccatcgccgc
    24121 ggcggcaacg gtgtgctgcg caaggacgtc gagtggatta tgcggaggtg tcaggttttc
    24181 cagcctgcct gcgaacatac actctacaat gactgcggaa tcgactaagt cacgcctggt
    24241 tcgggggaaa aaaagccctt tggagatacc tcctatctgg tgtccggcac gtccaatgcg
    24301 ttgtaaccca ctggcaacag aaagcggcgt tgccacctga atcaccagct ccacagcgcc
    24361 catatcaatc cccagttcga gactggaagt tgcgaccacg cagcgtaact cacccgattt
    24421 cagcgcctgc tcggtgattg ctcgttgttc tttagagacg gagccgtggt gtgagcgggc
    24481 aataaaaacg tcgctacttt gtacacggtt agaggttgcg ccagaggtcg actcgaaatg
    24541 ggccgcatca gcggcgatag acggggaacg ctgtaagcgt gcggcgtaaa gctcatttaa
    24601 tcgtgccgtc agtttttccg ccagcccccg cgaattagta aagacaatgg tcgagcgatg
    24661 gcgcaacact tcatcaagga tacccgtttc aatatatggc cagatggagc cttcccggcc
    24721 ggcatggctg tcttcgccag tgccgctggc gaccgatgag acatcatcca tattggcgac
    24781 tggtacgaca atccgtatct gcggatgacg cattgcgggc gggttgacta ccgtaaccgg
    24841 gcgatcgcca ccaagaaatg ctgccacatc gctggctgag cgcacagtgg cagaaaggcc
    24901 aattcgctgt gctgaggtgt ggagcagcgc atcgagccgc tccagactta acgccagatg
    24961 cgcaccacgt ttacttcccg ctaccgcgtg gacttcatca ataattaccg tttcgacgcc
    25021 gcgtagcgtt tcgcgcgcgc gggaggtcag catcagatag agtgattcgg gtgtggtgat
    25081 cagaatatcc ggcggattac gggtgagttt gctgcgttcc tgtgcaggcg tatcgccagt
    25141 acggatcccc acgcgaagat tgacttccgt ttcgccgcgc cgccgccgtt catcggcaat
    25201 acccttcaac gggatctgca agttgcgctg aacgtcggtg cccagggctt ttatcggtga
    25261 aatatagagg atgcgtgagg ttttacgttt atgcgcctcg cgggtatctt cgccgccttc
    25321 gcggaagagt cgatcgaggg cgtagagaaa tgctgccagc gttttcccgg agccagtcgg
    25381 tgcaatcacc agcgcatgtt cgcctcgcgc cgccacatgc caggtttgcg actggacagc
    25441 ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc gccggtgaaa acacgtccgg
    25501 caggggcgat gaagggtttg gattatctgc cattaacgct attccccgtg aatatcatgc
    25561 atgatgcgtg gaatagcctc actatagatg cctgaatcat tgcatgtcat caggcatcga
    25621 ctcgattaca cctcttcggc ggcaggtagc ggccagcctc ccagacgttt ccagcggttg
    25681 acgatttcac aaaacagcac cgcagtgcgt tcggtgtcgt acagcgcgga gtgcgcctgg
    25741 gtgctgtcga agtccatgcc agcggtctgg caagcctttg acaatacggt ttgtccgagt
    25801 gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag ggtggaacgg gttacgtttc
    25861 agtgaggcgc gttctgcggc ggccatcata aagctgtgat cgaaattggc gttgtgcgcc
    25921 accataatgg cgcggttaca gccgctcgct ttaatacctt tacgtacaac tttaaaaatt
    25981 tcgtgcagcg cctcgtattc gctgaccgcg ccgcgatcgg gatcgttcgg gtcaatgccg
    26041 ttgaaagcaa gggcttctgg ttgcaaattt gcgccgacaa atggttcgac gtggaaatgt
    26101 aatgtggtgt ccggcatcag ccagccttgt tcatccattt tcagggtgat ggcggcaatc
    26161 tcaagcagcg catcggtttt ggcgttaaat ccagctgttt caacatcgat cacaacagga
    26221 taaaagccac gaaaacggtc gcacagaccg gtaagttgag cgttatcgga catctgattc
    26281 tcttaatacg gataaattgc agcgcgcatt atgacaaata ttacttgtcg atgcagtaaa
    26341 gatgcgggcg cgatgagttc gcgcccggca ggagattagt tgcccagacc gcgaccggcg
    26401 tctttctctt cgattaactc aattttgtaa ccgtccggat cttccacaaa cgcgataacc
    26461 gtagtaccgc cttttaccgg acccgcttca cgggtcacgt tacccccgtt ttgacggatt
    26521 ttttcgcacg cttcagcggc attatctacg ctaagcgcga tgtgaccata agcagtgccg
    26581 agttcgtatt tatccacgcc ccagttgtag gtcagttcaa tcaccgcttc ttcggtttcc
    26641 gggccgtagc caacaaacgc cagtgagtat ttgtattccg ggttttcgct ggtacgcagc
    26701 agtttcatgc ccagcacttt ggtataaaaa tcgatggagc gttgcagatc gccaacgcgc
    26761 agcatggtat gaagaagacg catcttttta tcctcaaaat ggctaacaga ttcaaaatgt
    26821 tgttttagta tagcggcggc tttacgccgc tcgcaatgtt ggattacaac gtcgggtaat
    26881 cagtgtagcc ttccgcaccg ccaccataga aactttcagc acgctgtggg ttaagctcag
    26941 ctttgcgctg caagcgggcg accagatccg ggttcgcaat ccagtcacga ccaaatgcca
    27001 ccgcatcaat taaccctttg ccgatcagcg tttcagcttt ttctactgtg tatgcacctg
    27061 cgccgataat cggaccgtgg aaacgggcgc gtactttttc gcggaacgca tcagtatacg
    27121 gttcaccccc cgcccaatct ggttctgaca tatgcagata agcaatgccg cgtttaccca
    27181 gttgttcaat cagatacagt gcatcggctt cttcattcgg gccgttatcc gtgttctgga
    27241 aagtaccgat tggcgaaacg cgaatgccaa tgcgatcggc accccattct tcaatcccgg
    27301 catcgaccac ttccagtacc aaacgtgcgc gattttccac gctgccgccg tactgatcgg
    27361 tacgatggtt tgaagaagga gaaaggaact gatgcagcaa ataaccatga gcagagtgga
    27421 gctctaccag atcaaaaccg gcttcacgcg cgttagcaat agcctgacgg aaatcattga
    27481 cgatacttgg aatctcttcc agttcaagcg cacgcggcat ggatgtttca acacggatcg
    27541 cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc gctaagtgct gaaggcgcta
    27601 ctggtgcctg gccgccaggt tgcaggctgg cgtgagaaat gcgtccggtg tgccacagct
    27661 gtacggccat atgcccattt tcagcatgaa cgccagcggt aatttttttc catgcggcaa
    27721 tttgttccgg actatggatg ccaggtgcac cagcataccc ttttgcctgg gcagaaattt
    27781 gcgtggcttc gctaataatc aaaccggcac tggcacgttg gcgatagtat tccgccatca
    27841 acggggtagg gatgtcaccc ggttcaatac tgcgcagacg cgtcagcggt gccataaaaa
    27901 tacggtttgc cgcagttatt gcgcccactt tcagtgggga atacagtttt tcagatgaca
    27961 taaaggctcc cggtcgtctg gtgataaaaa atgcctgcta aacggcaggc gtcgcaataa
    28021 tggtttttac atgtgccagc gcgttttcca gtggctcgga attgcgcgaa attttggcct
    28081 gcagattcgc acccagccat agtgcgtaaa gcacttgtgc ctgttgcagc ggttcgccac
    28141 aaaaggttaa acaatggcta tcacggccat tttccagagc ctgcgagagc agggcgatca
    28201 cgccgcgagc gcctttatcc atcgcgctac gcatatcttc tgacagatcg cacacttcgg
    28261 cagagagttt tactgtcagg caaccactga tggttccatg ttggcaaaac tggttcagtg
    28321 tttgctggta ataagccagt atgcggtcgc ggtagttacc ttcgccggat tgcagcaact
    28381 cagtcagtcg ttggtgatat gtggcgtaat ggcgctcaag catggcaacg ccaaacgctt
    28441 ctttagagcg aaagtagtga tagaaggacc ctttcggcac ttcagcggtt tttagtaatt
    28501 cgcttagccc catcccggtg aatccacgtt gcaggcaaag ttgctcgccc gtcgccagga
    28561 gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg ttggagtgta gtagaccagt
    28621 cggtctagtg caagcaaagt tgctactttg ggcattaaca gcttcaataa tggtaataat
    28681 cttatttgag gagggcgaag tggcggagca attagagttc tttcccgtcc agagcccgtg
    28741 ccggggaatt tgccagtctg acgaacgcgg gttttgtcgc ggctgttttc gaagccggga
    28801 tgagcgtttt aactggaata aaatgagcga tggcgaaaaa caagaggtgc tgcgcctttg
    28861 tcggcagcga ttaatgcgca aattacgtgc aaataagcca gcgtcatcag acgaaccgga
    28921 gcaaccatca ctcttttgag cctaatcttg cgtatactcc tctcatatct gataagagga
    28981 agcgattatg gttcagcgta ttactattgc gccgcaaggc ccggagtttt cccgttttgt
    29041 gatgggctac tggcgattga tggactggaa catgtccgcc cgccagctgg tcagttttat
    29101 tgaagagcat ctggatctcg gcgtgaccac cgtggaccat gctgatattt atggtggcta
    29161 tcagtgcgaa gcggcgtttg gcgaggcgct gaaactggca cctcacctgc gtgagcggat
    29221 ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgcgt gaagaaaacg tcattggtca
    29281 ttacatcact gaccgcgatc acatcattaa gagcgccgaa cagtcgctaa ttaatcttgc
    29341 gaccgatcat ctggatctgc tgttaatcca ccgcccggac ccgttaatgg atgccgatga
    29401 agtggcggac gcgttcaaac atctgcatca gagcggcaaa gtgcgtcatt ttggcgtatc
    29461 caacttcacg cctgcgcaat ttgccctgtt gcaatcacgt ctgccgttta cccttgccac
    29521 taatcaggtg gaaatatccc cggtacatca gccgttactg ctggatggca cgctcgacca
    29581 actgcaacaa ctgcgtgttc gtccgatggc gtggtcctgc cttggtggtg gtcgtctgtt
    29641 taatgatgat tatttccagc cgctgcgtga tgaactggct gtggtggcgg aggagttaaa
    29701 cgcgggctcg attgaacagg tggtttacgc ctgggtatta cgtttaccgt cgcagccgct
    29761 gccaattatc ggctgaggta aaattgagcg cgtacgggca gctgtcgaag cagaaacact
    29821 gaaaatgacc cgtcaacaat ggtttcgtat ccgtaaagcg gcactggggt acgacgtacc
    29881 gtaagcactt ttaggaatag ccgccgttca aaaatgtgtc actggtttac acttattcag
    29941 gaatgcacaa tgaacggagg tcctatgaaa cgttttagtc tggccattct ggcgctggtt
    30001 gttgcgaccg gcgcacaagc tgccagtgaa aaagtcgaga tgaacctcgt cacgtcgcaa
    30061 ggggttgggc agtcaattgg tagcgtcacc attactgaaa ccgataaagg tctggagttt
    30121 tcgcccgatc tgaaagcatt acctcccggt gaacatggct tccatattca tgccaaagga
    30181 agctgccagc cagccaccaa agatggcaaa gccagcgccg cggaatccgc aggcgggcat
    30241 cttgatccac aaaataccgg taaacatgaa gggccagaag gtgccgggca tttaggcgat
    30301 ctgcctgcac tggtcgtcaa taatgacggc aaagctaccg atgccgtcat cgcgcctcgt
    30361 ctgaaatcac tggatgaaat caaagacaaa gcgctgatgg tccacgttgg cggcgataat
    30421 atgtccgatc aacctaaacc gctgggcggt ggcggtgaac gctatgcctg tggtgtaatt
    30481 aagtaattaa gaggccagcg taccttgcgg tggtgcttgc tcaagttgcg aaagcgagca
    30541 gtacagccgc cagacaattg ccgccagctc acgggcggca ggttgatgat ggcgggcaag
    30601 gctgtcgcaa atccgctgta attcttcaag tgtggccgcc agtgattttt gctgaacgcc
    30661 acgctcactc atcactcccc gcaacagtga aatacagtta tcccgtactc gcgataacgg
    30721 atcggaacgc gattcccagt cgcgtaattg ccagacaaca tgagaacagt tcagcagcac
    30781 tataccccag cgtaataacc aacggcgagc cagcgcatcc tggctgttac tcaactgact
    30841 gacgtgatga taagtgagcg attcaaattc gctttcactc agtgttggat ggcggcttag
    30901 ttgatcgaca aaatcccggc gcagcgcgcg aatatggcgg cggcttttgc gagcatccga
    30961 tcctggacgc agaatggcga acgctaacca cgccaacgcg acgccaacga tttttgccag
    31021 attatcgtta agaaaatcag caaaattata caccggggga ttagtgacgg cgataaaaga
    31081 acccataaaa acaatcagtt gcccccacaa tgcggcaaat ttgggcatct gcaatttaag
    31141 aagctgcatt gtcgccagca gtggaaagag aaacagtaaa aattgccaca gatcgctaat
    31201 ctggaccatc agaccaaatt tgaccacaaa gctgaatagc gaaagtaaca ccagcgtgcg
    31261 catcagcagt gacaacgact taaacggtgc tgcgacggcg gagtagagta cgcagctaat
    31321 cgctgccagc gttaatgcat tggcaccggc atcccattgc gaagcaatac tccatgcgcc
    31381 aatcatcatc aacgtacaaa atgtacgcag tccgctccac atagcttcgg cgttatcggt
    31441 atgacgagcc aggccggatg tccgtgggag tctggcatga tcatctacat cgctttgcag
    31501 acgatgtaat tcctgactac tttgcagata aaggcggcaa aaataacgta gtcgctgcca
    31561 gaaggcgacg tgccgatagt cggcgacgtt ggtcgggcgt agcggggcga taatacgtgc
    31621 gacggtgtaa acatctgttt gcgaactggc gagcgccgtc agcaactgtt cgagaatttc
    31681 tcgcgtggca cctggcggtg agggccagtt gagcaacata cgtcgcaggc tggagatgac
    31741 actggtcata cgtaattgct ggtggagcag cgcattaagg cgcgcgtttt gctggcgaaa
    31801 acgatagtgg ctccagaaag cctggatacg cagcaaattc atggtcagta tctgcccaat
    31861 caccccttca tgtgctgcac gaatggcatc ggttgtttca ggctgccaga gtaaactggc
    31921 atgttccagt aatcgggcgt gcatgttttt caatgcggtt aaaagggctg tagcatcgga
    31981 actgctaggc aggatcatca tcatcatgcc gccgcacaaa ataccgacaa tcacctcgca
    32041 aacgcgcgcc tgagcgatat cccacagctg gctggcctca gtaatattaa ccatcggaaa
    32101 ggcgatgatg gcagccgtgt agcccgccag ttgaaatgca tacgcgacgt tattcgtgaa
    32161 gtgcgcacag gcccaggtac aaaagccaag ccacgccgac atgctcaata gaaaaaacca
    32221 cggctcattg agcgtatgcc cggcaagaag cagtgccgca atggctccca gtaaactgcc
    32281 agcgatgcgt ccgaggcttt tgctgatgac accgccaacg gtgggaaagc taaccactgc
    32341 agccgaggtc atcgcccaat agggttcatc cagattcaaa taataggcaa ccgtcagcgc
    32401 cagacacatg gcaatggtat tgcgtaacgc ataacgccat tgcgccagcg tggccctgaa
    32461 ccagggcaaa ttgcgcaagg accatgacga tgcgttcatt attgacctac ggcaatgcta
    32521 caggtggtgc ccgacaccag cgtgacatcg cctggaactt tatccagcgc aaagcggacg
    32581 ggaacgcgtt gggcgagacg aacccagggg acgttaggtt tcacatccgg aataaggctt
    32641 gagtcacttt caacgctctg atcataaatc gcccgaccga tactggaaac gtgaccctgt
    32701 aacgttttgt tatcactata gagggtaatt tgtgctggtg caccttcgcg gatatggcgc
    32761 aacttggttt cttcaaaata accaatgacg taaaacgagt ggctgtcgac aagggcaaac
    32821 agcggtttcc cggtgtcagc gtaatcgccg atgcgggtag tgagattagt cacccatcca
    32881 ctcaccgggg cgcgaatttc cgtttgcgcc agttgccatt gtgtctgttt cagcgtcgcc
    32941 tgtgcggcat caacactggc ctgcatcgct ttaacattga ggttagcggt atcgagttct
    33001 tcggcagaaa taaaattttg tgagagatga cgacgacgat tcgcttcgtt attggctttt
    33061 gcgaggtcag attgcgcttt cgccagctga gcctgtgcat tcagctcggc gatctgaaag
    33121 ggcgttttat cgatcgtgag cagaagatcc cccgcgttaa ccagctggtt gtcttttata
    33181 ttcagctcaa caatacgtcc cgacacctgt ggggtgatag aaacctgttc tgcgcgtatt
    33241 ttcccgtcgc gtgtccacgg agattgcatg taataattcc acagccacca tccggcaaga
    33301 accgcaacta ccgctacaat gattgttgag aaatacttaa ttgttttaat tgacatagtt
    33361 accacgcaat cagtattgcc agagcaaggc aaacgcaaat cgcaaacagc gataaatcca
    33421 ttaacaaggg atgccagatg tcaccggcgt agatccagcc acgaagcagg cggtgtacga
    33481 caagccagat gacgaatcca aacgcgaagg ctttgaaaaa cggaggaaag tagacggacg
    33541 caccgaacac cagatcttgt aagggcaatc ctgttgcatt gagcataaac ttcacaaagt
    33601 agattccttt acgaccgaat atattgcgtt agattaataa atattcttta actgcgaaaa
    33661 atttacgcgc aatttctgaa aaatatgtct gacggtaacc aaatgcagca atacatttgt
    33721 tttagcaata caattgctgc acactattct aaaagccgca taatatctta gcaagctaat
    33781 tataaggaga tgaaattgga atcgccacta ggttctgatc tggcacggtt ggtgcgcata
    33841 tggcgtgctc tgatagacca tcgcctgaaa ccgctggagt taacacaaac ccattgggtt
    33901 acgttacaca atatccatca gttacctcca gaccagtcgc aaattcaact ggcaaaagcg
    33961 attggcatcg agcagccatc actggtccgt actctggacc aactggaaga aaaagggtta
    34021 atttcgcgtc aaacttgtgc cagcgatcgt cgggctaaac gtattaaact gacggaaaag
    34081 gcagagccgc tgatcagcga aatggaagct gttattaaca aaacccgcgc ggaaatttta
    34141 catggcatct ccgcagagga actggaacaa ctgattacgc tcatcgcaaa actagagcat
    34201 aatatcattg agttacaggc caaagggtga aatgaagggg gcgtgtggtc agtaacctga
    34261 ccacacgcaa acttatttag cgcggagaaa cggtcacctg actgccattg ctggccagta
    34321 cgacacgttg gcccggagag aaacgagtgt tgccttgttt ctgtaccacc atgatggtat
    34381 taccatcgtc tttacgaatt tccagctcga caccttgcgt tttgttcatt gcactctgta
    34441 cgccctgacc agctacgcca cctgcaacag cgcctgctgc agtagccaga gaacgcccgg
    34501 ttccgccacc aacagtattt cccaggaaac caccaagaac agcaccgcca attgcaccga
    34561 taacgttgga atcatcaccg ccctgaatct gtaccggacg tacgttaacg atggttccat
    34621 agctgacatt ctgtacttgt ttcgcttcag aagcggtata aacatcccct gacagggtgt
    34681 cgttattaac acaaccgaca agagacagac ctaccattga aacaaccaat acgcgtttaa
    34741 tcattgaaaa atctcctgtt caccatgaaa cgccacgcaa gcatccctca tggtcaaagt
    34801 atatggcata tgtgaatcca tggttacatg ttctaccaaa tatgagcaaa tcataatgaa
    34861 agtaggatca accaattata aacaaaacat tcatgatgat tattgatttg aagataatgt
    34921 tttactacca acggtaaaaa tcggtcattc cgcatggtat tcacgaaaag tttatgtttg
    34981 agtgtgcgca aacgtcagat tgaaaaagga tgaactatga aatcgggccg ctttattggc
    35041 gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt tggcgacaat tgatgaacac
    35101 cgggtcgcac agctggcaag tttgagctgg ccgatcccgg tgtctctgaa acaggctgta
    35161 ctggatattt gccagggcca gcagcttaca ctttcgcagt ttggacagct tgatactcaa
    35221 ctcgggcgac tttttgctga tgcggtcaag gccttgctta aggaacaaaa cctgcaggcg
    35281 agagatatag ttgcgatcgg ctgtcacggt caaaccgtct ggcatgaacc gacgggcgtg
    35341 gcaccacaca ctttacagat tggcgataac aatcaaattg tggcacgcac cggaattacg
    35401 gttgtcggtg attttcgccg tcgcgatatt gccttgggag gacaaggcgc accgctggta
    35461 cctgcgttcc atcatgcctt gctggctcac ccaaccgagc gacgaatggt gctcaatatt
    35521 ggcggcatcg ccaatctgtc actgctcatt cctgggcagc cggttggcgg ctacgatacc
    35581 ggtcctggta acatgctgat ggatgcctgg atctggcgtc aggccggtaa accttacgat
    35641 aaagatgccg agtgggcacg ggcgggtaaa gttattctcc cactgctgca aaatatgctc
    35701 agcgacccat atttctcgca acctgcaccg aaaagcaccg gacgcgaata ctttaactac
    35761 ggctggctgg agcgccattt acgccatttt ccgggtgttg atccccgtga tgttcaggcc
    35821 acactggcag aactcaccgc cgtgaccatt tctgaacaag ttttgttgag cggtggctgc
    35881 gaacgattga tggtatgtgg tggaggtagt cgtaatccgc tactcatggc gcgtctggcg
    35941 gcattactgc caggcacaga agtcaccacc accgatgccg ttggcattag tggcgatgac
    36001 atggaagcat tggctttcgc ttggcttgcc tggcggacgc tggcgggatt accaggaaat
    36061 ctgccttccg tcactggcgc aagccaggag acggttctgg gggctatttt ccccgccaac
    36121 tcgtgatatc agaattaact gaattttttc ttatcgtgtc tgccgttaca cttggaagat
    36181 taaggaggat atttgccctc cggaccagga aagtcttcgg gatatgacta tgaaaaaact
    36241 gttaattatc attttgccgg tccttctctc cggctgtagc gcctttaatc aactggttga
    36301 gcgaatgcaa accgatacgc tggaatacca gtgtgatgaa aaaccgttga cggtcaaact
    36361 gaataatccg cgccaggaag tcagttttgt ttacgataat caactactgc atctcaaaca
    36421 gggcctttca gcctctggcg cgcgttactc cgacgggatc tatgttttct ggtcgaaagg
    36481 tgaggaagcg actgtctata aacgcgaccg catcgtcttg aataactgtc agttacaaaa
    36541 tccacagcgt tgagattttt ccaggggcgg cgcacaatag cgtcacccac agacaatccg
    36601 taaagaaaac catgtctgat aacgacgaat tgcagcaaat cgcgcatctg cgccgtgaat
    36661 acaccaaagg cgggttacgc cgccgcgatc ttcccgccga tccattaacc ctttttgaac
    36721 gttggctctc tcaggcttgt gaagccaaac tggcggaccc taccgcgatg gtggtcgcta
    36781 ccgtggatga acatggtcag ccttatcagc gcatcgtttt actcaaacat tacgacgaaa
    36841 aaggcatggt gttttacacc aacctcggta gccgtaaagc acatcaaatt gaaaataatc
    36901 cgcgcgttag cctgctgttc ccgtggcata cccttgaacg ccaggtgatg gtgatcggta
    36961 aagcagaacg actttcgact ctcgaagtga tgaaatattt tcatagccgc ccgcgtgata
    37021 gccagattgg ttcatgggtt tcgaagcagt ccagtcgcat ttctgcccgc ggtatccttg
    37081 aaagtaaatt cctggagctg aagcagaagt ttcaacaagg cgaagtgcca ttgccgagct
    37141 tttggggcgg ttttcgcgtc agccttgaac agattgagtt ctggcagggc ggtgagcatc
    37201 gcctgcatga ccgctttttg taccagcgtg aaaatgatgc gtggaagatt gatcgtcttg
    37261 caccctgaaa agatgcaaaa atcttgcttt aatcgctggt actcctgatt ctggcacttt
    37321 attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc ggcaaaggtg cagtcgttat
    37381 atacatggag attttgatgg caagcagtaa cttgattaaa caattgcaag agcgggggct
    37441 ggtagcccag gtgacggacg aggaagcgtt agcagagcga ctggcgcaag gcccgatcgc
    37501 gctctattgc ggcttcgatc ctaccgctga cagcttgcat ttggggcatc ttgttccatt
    37561 gttatgcctg aaacgcttcc agcaggcggg ccacaagccg gttgcgctgg taggcggcgc
    37621 gacgggtctg attggcgacc cgagcttcaa agctgccgag cgtaagctga acaccgaaga
    37681 aactgttcag gagtgggtgg acaaaatccg caagcaggtt gccccgttcc tcgatttcga
    37741 ctgtggagaa aactctgcta tcgcggcgaa caactatgac tggttcggca atatgaatgt
    37801 gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt aaccagatga tcaacaaaga
    37861 agcggttaag cagcgtctca accgtgaaga tcaggggatt tcgttcactg agttttccta
    37921 caacctgttg cagggttatg acttcgcctg tctgaacaaa cagtacggtg tggtgctgca
    37981 aattggtggt tctgatcagt ggggtaacat cacttctggt atcgacctga cccgtcgtct
    38041 gcatcagaat caggtgtttg gcctgaccgt tccgctgatc accaaagcag atggcaccaa
    38101 atttggtaaa actgaaggcg gcgcagtctg gttggatccg aagaaaacca gcccgtacaa
    38161 attctaccag ttctggatca acactgcgga tgccgacgtt taccgcttcc tgaagttctt
    38221 cacctttatg agcattgaag agatcaacgc cctggaagaa gaagataaaa acagcggtaa
    38281 agcaccgcgc gcccagtatg tactggcgga gcaggtgact cgtctggttc acggtgaaga
    38341 aggtttacag gcggcaaaac gtattaccga atgcctgttc agcggttctt tgagtgcgct
    38401 gagtgaagcg gacttcgaac agctggcgca ggacggcgta ccgatggttg agatggaaaa
    38461 gggcgcagac ctgatgcagg cgctggtcga ttctgaactg caaccttccc gtggtcaggc
    38521 gcgtaaaact atcgcctcca atgccatcac cattaacggt gaaaaacagt ccgatcctga
    38581 atacttcttt aaagaagaag atcgtctgtt tggtcgtttt accttactgc gtcgcggcaa
    38641 aaagaattac tgtctgattt gctggaaata atgcattaag tggaaagggg gagtgagaaa
    38701 tcactccccc tggtttttat acagggaaca tgatgaaaaa tattctcgct atccagtctc
    38761 acgttgttta tggtcatgcg ggtaacagtg cggcagagtt tccgatgcgc cgcctgggcg
    38821 cgaacgtctg gccgctgaac accgttcaat tttctaatca cacccaatac ggcaaatgga
    38881 ctggctgcgt gatgccgccc agccatttaa ccgaaattgt gcaaggcatt gccgccattg
    38941 ataaattaca cacctgtgat gccgtattaa gtggctatct gggatcggcg gagcagggtg
    39001 aacatatcct cggtatcgtc cgtcaggtga aagcagcgaa tccgcaggcg aaatattttt
    39061 gcgatccggt aatgggtcat ccagaaaaag gctgtatcgt tgcaccgggt gtcgcagagt
    39121 ttcatgtgcg gcacggtttg cctgccagcg atatcattgc gccgaatctt gttgagctgg
    39181 aaatactctg tgagcatccg gtaaataacg tcgaagaagc ggttctggca gcgcgcgaac
    39241 tcattgcgca agggccacaa attgtattgg ttaaacacct ggcgcgagct ggctacagcc
    39301 gtgaccgttt tgaaatgttg ctggtcaccg ccgatgaagc ctggcatatc agccgtccac
    39361 tggtggattt tggtatgcgc cagccggtag gtgttggtga tgtgacgagc ggtttactgc
    39421 tggtgaaact gcttcagggg gcaacgctgc aggaggccct ggaacatgtg accgctgcag
    39481 tctacgaaat catggtgacc accaaagcaa tgcaggaata tgagttgcaa gtggttgctg
    39541 ctcaggatcg tattgccaaa ccagaacatt acttcagcgc aacaaagctc tgaaatatct
    39601 catttcggcc cacaacgaac tgtgggccga cggctattac tttaagcctt ccgctgacag
    39661 cgcgtcttgt acttccggac gttcagccat acgttgcata aatgccgcaa tgtgctctaa
    39721 cccttccaga ttcagtttca ccgcgtatgc ccagcgcagt accgtaaaca gataggcatc
    39781 agcaattgta aatctttgcc cgcagatcca gtgctcatcc ttcagtgcct cgttcacata
    39841 ttgcagtttc ttctccagct gcgcgcgaac tgtcggtttg tactcttccg gtgtatcagg
    39901 gcgaaacaga ggtgtgaaac ctttatgcag ctcggtggcg atgtaattca gccattcgat
    39961 ggttttatag cgggaaatac tgtttaccgg tgccagcaac tggcggtcgg ggacgctgtc
    40021 ggcaagatac tgcataatcg ctacaccttc cgtcagcaaa gtaccgtcat ccagcagcaa
    40081 tgcaggtacc tgtcccttag ggttaacggc aaagtaatcg tcaccgtttt cgagacgttt
    40141 tttcattaaa tccacactga cgagcgtaaa atcctttccg ctctcacgca gggtgatatg
    40201 ggaagcgaga gagcaggcgc ccggtttgta gaacaatttc atcggtaact ccttttgtct
    40261 gtggtttaaa gtatggtagt gcgttgctga acaaaaaaaa agccgctaac gaaaagttag
    40321 cggcttgtaa aagagtttcc ctgaatttta cgctacggct gctttcgccg ctttgtctgc
    40381 agcgtcatcc tgcgtcatgc ggtgcagttt cggcgcggtc agcagcatca gtactgcaat
    40441 aacggcagta gcgacaccaa tctgcaagaa tacgcgacca tagacttcca gtgacatcag
    40501 cggatcggta acgttatccg gcacagccat catacccgca acataaccac caatcaggtt
    40561 tgcaccggca gtggtcagga accagctacc cataatgaag cccatcagac gctgcggaac
    40621 gagttgtgca accattgcca gacccagacc agagatcatc agttccccga tactctgcag
    40681 gccatagctc gcgaccagcc agcttacaga cacgatacct gcgtcagacg cgaatttcgc
    40741 acccagcggc agaatcagga acgcaccaga acacatcacc ataccgattg caaacttggt
    40801 tggcatcggc agggtatcgc ccatcttgtt atagatagcg gccagaatcg ggctaccaat
    40861 gatgatccag aacgggttca gtgcctgata ctgctcaggt tctacggcca ggcccagaat
    40921 ggtatgttca acgttacgaa tcgcaaagaa gttcagtgac gttggcatct ggctgtacag
    40981 cacgaagaag ataatggctt cgagcatcag gatgaaggca acgatcattt tacgacgcgc
    41041 agcacctttc atcgcgaagg cttctttacc gaagataacc acgataccga aggcaacaac
    41101 gcccagcgcc atacgcgcaa cttcctgatt gtgcagcagc caggtggcga tagcgatcag
    41161 tgccacaaca ccaataatgg tcagcagcag gttacggtag ttgataggct cgaagtctgg
    41221 ttttgaaccg tactgtttaa cccagcgttg gcagaaggcg aagttaacga tagtgatcag
    41281 caggcctaca acgctcaacg caaacgcaac actccagccg tatttcgcgg ccagccacgg
    41341 ggtggcgatc atagagaaga aagagccgat gttgacggac atgtagtaca tggtgaatgc
    41401 accgtccaga cgcgggtcgt ttttctcata gcatgtagaa agcagagaag acgggttagc
    41461 tttaaacagg ccgttaccga ccgcaatagc cgccataccc atataaacga taccggcgtc
    41521 gtgaccagac caggcaacca gagcataacc aatcgccagc acaatagcgc cgagcataat
    41581 tacgcgttta gtacccagta ccttgtcacc taaccagccg ccgatagcga ccagaccata
    41641 aaccagggca ctaaaggaag agaaaagggt gattgaatcc gcttcagaca tacccagttg
    41701 tttaaccagg taaacagcca taattccttg taggccgtaa taaccaaaac gttcccataa
    41761 ctcaatcgag aagatgagat agaacgcctt cggttgtttg aaagcgttca aactgacgct
    41821 ttcagttggt ttttggtttg cagtggacac ttttacctct ttttttacat cccatattaa
    41881 cgggggtgct ttctcgcgtt atgtctaaaa aacatacgcg caattgttat agtgggaggg
    41941 gaaacggcag gtaatgttca ctatcttgca ctttcagaca agtcctttgt aataatctgt
    42001 tacatataac tgaggcgggg aattcaacca ttccgggaaa tgttatccag cgttaaattt
    42061 ttcaaaaaac gtaacggcag attttttcac tgcattgaga gggggatgat ggcttgacgg
    42121 cgatatgttc tgctatttag cgtttttcct agtgagatag tccagtttct taaaaatagc
    42181 cagtgtaatg ttttgcaggt caatatttgt tgcgtgtgta aatttaaatg attttaacct
    42241 gattcttgca atgtttttac attgccacag catcgtgatc ttgatcacta atgaatagaa
    42301 aatttcgcta ttaaaaaaac aattaacctg catttttggt tatccgcaaa cggattacct
    42361 gatgataacg ctaagcaatg gcatcattga cgttaaaaag atgttttaaa agaggataaa
    42421 gaaaggttat caatggggta atcggtgtta ccccttttct tcagatgtca actttctctt
    42481 tgtattcaca aagatcttca ataatacaag agccacagcg gggcttgcgg gcaatgcagg
    42541 tataacgccc gtgcaggatc aaccaatggt ggcagtcgac tttaaactct gccggaacca
    42601 ctttcagtag cttttcttct acctgttcga cgtttttccc cggcgcaaat tgagtacgat
    42661 tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg ccagccgaat gcagtgttta
    42721 atacgacgtt ggctgtttta cgacctacgc cgggcagggc ttcaagcgca gcacgatctt
    42781 ccggaacctc gctattatgc tgctccagta agatacggca ggttttgatg atattttctg
    42841 ctttgctgtt ataaagccca atcgttttga tataggtttt caccccttca acgcccagtt
    42901 caagcatcgc tgcaggcgta ttcgccaccg ggtagagttt cgccgtcgcc ttattaacac
    42961 tgacatcggt tgcctgagcg gaaagcagta cggcaatcag caattcaaaa ggcgaactga
    43021 aattaagctc agtggtggga tgaggattgt tctcacgcag gcgagtgagg atctccaggc
    43081 gttttgcttt gttcatcaga cattccctgt ttcaccgttt ggcaatgcac gttcggcagc
    43141 tgcttcagta cggcgctttt tcattctttc atcaatcagg tattttcctg ccagcatcag
    43201 tcccaggcca ataaatgcac ctggtggcag catcgccagc aggaaagggg agtcggtgtg
    43261 gaaaatctcc acgcgtaata cttttgccca gctacctaac agcgcatctg caccgtcaaa
    43321 caatgtgcca ttgccgataa tttcgcgtag tgaacccagc acgaacatgg cgcaggttgc
    43381 gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc ggaccttttt tggcagcgaa
    43441 ggcttcagcg cggcccacaa cgatacagtt agtgacaatc agcggaataa aaatccctaa
    43501 tgattgatac aggccaaagg cgtaggcgtt gatcagcatc tgtacagcgc tgaccaccga
    43561 ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct ggcgtccagt gacgcagcgt
    43621 cgaaatggtc aggttggtca gcgtcagtac cagcgtagtc gcaagtccta aacccagagc
    43681 gttagtggca gtagacgtga cagccaacag aggacaaagg ccgagcaact ggaccagcgc
    43741 agagttgttt ttccacaacc cctgaacaat aacgtcttta atttcgctca cgctttattc
    43801 tccacaggca ggaagttgag aaagttgttc cggtaacgtc tgagcgtaca atccggcgcg
    43861 ttttaccgca ttaaccaccg cgcggggagt aatcgtcgcg ccggtgaact ggtcgaaatt
    43921 accaccatct ttcttcaccg cccagtgcgc atcatccgca ccactgattt ttttacccgc
    43981 aaaatgggtg atccagtcag aaaggcgcag ttcgatttta tcgccaagcc ctggcgtttc
    44041 gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta aaatcggctc ccaccagcag
    44101 ctgaatcgca ccggaatagc catctggcgc ggttgcttcc agaacggcgg ctaccggttt
    44161 gtcatcctgt ttggcgatgt aaacccgatg ctcaccttta cctaactctg gcgcagttac
    44221 cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg gctggcagca cctgatcaaa
    44281 taaagccttt tgttgcagac tggcctgttc agcaatcgtc gttttggtca tctggttgat
    44341 ggccgcagtt aaccctgttg aacccgctgc aaatagcgcc aacgtaatgc cgtgttttcg
    44401 gatagttttc agcatggttt accctttgcg atggccgtag acgcgcggac gcgtgtagta
    44461 atcgatcaga ggaaccgtga tgttcgccag caggacggca aaagccacgc cgtcaggata
    44521 gccgccgaaa ctgcggatca accagactaa taagcccgca agcgcgccga aaataagacg
    44581 accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa ataaagaatg cgccgagcat
    44641 ggtcgctcca gacagcagat gaatttgcgg tgctgccagt gtttctggtg agaacaacca
    44701 gcccaacgtt gcgcataacg ccagcgttac taagaagctg aggggaatat gccagcgaat
    44761 cgctttctgc catagcaacc atacgccgcc agccagccag gcgagattta cccattgcca
    44821 accagcgccc gccagaatac cgctgtagat cggatattgc ataatctgtt caaccgaatg
    44881 accggcacgg acagaggttt taaatgtatc cagcggtgtc gcctgactaa tgccatcaat
    44941 acctaagcgt agtgtgttca tatcaccacc actggcggta tgcccgctga aaataacctg
    45001 tatggcgtcg ataaaaccag ggatgttgac cgcaatttca tgtggcggta accagctggt
    45061 catctgcacc gggaaggaga tcagtaagac cacataacca atcattgccg gattaaacgg
    45121 gttttgcccc agaccgccat acaactgttt agcgatgata accgcaaaca ccgtacccag
    45181 cacgaccatc caccatggcg cgaggggggg aatacttacc gccagcaata agcctgtcag
    45241 caatgctgag ttatctttca acgtggcggc taccgactgc ttgcgtagtt tgagtacgag
    45301 agcttcggct aacagagcac taaccgatgc caacaggatc tgaacgagag taccccaacc
    45361 aaaaaaccac agttgcgctg cgattcctgg cacggctgcg agcaacacca acagcataat
    45421 gcgcgatgtc tggcgctggt tatgggtata aggggagcta gctattctga ataccattta
    45481 gtcctcgttt acaacctttt gctggacggc ttttttggcc tgaacgcggg caatagccgc
    45541 ggcaactgcc gctttgcgcg gatcaacctg ttcttctggt tctgcattag cctgttgctg
    45601 ttccagcttg cgtgctttgg cgcgtgcaat agccgctgcg acggcagctt tgcgcggatc
    45661 aacctgttct tctggctccg cattagcctg ttgctgttcc agtttgcgcg ctttggcacg
    45721 ggtaatagcg gcttc
//
LOCUS       DAZKIF010000018        45507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZKIF010000018 DAZKIF010000000
VERSION     DAZKIF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.018"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(100..933)
                     /gene="fdhD"
                     /locus_tag="ACHOTW_001080"
     CDS             complement(100..933)
                     /gene="fdhD"
                     /locus_tag="ACHOTW_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP9843234.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1127..4177
                     /gene="fdnG"
                     /locus_tag="ACHOTW_001081"
     CDS             1127..4177
                     /gene="fdnG"
                     /locus_tag="ACHOTW_001081"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1712..1714,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9843235.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQENYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4190..5092
                     /gene="fdoH"
                     /locus_tag="ACHOTW_001082"
     CDS             4190..5092
                     /gene="fdoH"
                     /locus_tag="ACHOTW_001082"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP9843236.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            5089..5724
                     /gene="fdoI"
                     /locus_tag="ACHOTW_001083"
     CDS             5089..5724
                     /gene="fdoI"
                     /locus_tag="ACHOTW_001083"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9843237.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5721..6650
                     /gene="fdhE"
                     /locus_tag="ACHOTW_001084"
     CDS             5721..6650
                     /gene="fdhE"
                     /locus_tag="ACHOTW_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP9843238.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6980..7222)
                     /locus_tag="ACHOTW_001085"
     CDS             complement(6980..7222)
                     /locus_tag="ACHOTW_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9843239.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7439..7657)
                     /gene="yiiE"
                     /locus_tag="ACHOTW_001086"
     CDS             complement(7439..7657)
                     /gene="yiiE"
                     /locus_tag="ACHOTW_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9843240.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(8351..9265)
                     /locus_tag="ACHOTW_001087"
     CDS             complement(8351..9265)
                     /locus_tag="ACHOTW_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9843241.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEQPLFVKHTAMMREACKELPQLVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKKRMAELFNQSDTSVRNKYRPAFDKWNKLV
                     DKLNHERNTVPFACLTTIIATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            complement(9272..10165)
                     /locus_tag="ACHOTW_001088"
     CDS             complement(9272..10165)
                     /locus_tag="ACHOTW_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843242.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNESPKQSLLNDFSHRLKMEG
                     EEQLFHCVNELKYGLSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDVAPDILV
                     LPVNVYHADFKIKINNAVEKT"
     gene            complement(10576..11517)
                     /gene="fabY"
                     /locus_tag="ACHOTW_001089"
                     /gene_synonym="yiiD"
     CDS             complement(10576..11517)
                     /gene="fabY"
                     /locus_tag="ACHOTW_001089"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP9843243.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11562..11999)
                     /gene="dtd"
                     /locus_tag="ACHOTW_001090"
     CDS             complement(11562..11999)
                     /gene="dtd"
                     /locus_tag="ACHOTW_001090"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9843244.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11996..12868)
                     /gene="yihY"
                     /locus_tag="ACHOTW_001091"
     CDS             complement(11996..12868)
                     /gene="yihY"
                     /locus_tag="ACHOTW_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP9843245.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12862..13461)
                     /gene="yihX"
                     /locus_tag="ACHOTW_001092"
     CDS             complement(12862..13461)
                     /gene="yihX"
                     /locus_tag="ACHOTW_001092"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP9843246.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13560..14345)
                     /gene="csqR"
                     /locus_tag="ACHOTW_001093"
     CDS             complement(13560..14345)
                     /gene="csqR"
                     /locus_tag="ACHOTW_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9843247.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(14379..15275)
                     /gene="yihV"
                     /locus_tag="ACHOTW_001094"
     CDS             complement(14379..15275)
                     /gene="yihV"
                     /locus_tag="ACHOTW_001094"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFP9843248.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            15443..16339
                     /gene="yihU"
                     /locus_tag="ACHOTW_001095"
     CDS             15443..16339
                     /gene="yihU"
                     /locus_tag="ACHOTW_001095"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP9843249.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            16363..17241
                     /gene="yihT"
                     /locus_tag="ACHOTW_001096"
     CDS             16363..17241
                     /gene="yihT"
                     /locus_tag="ACHOTW_001096"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFP9843250.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            17257..18498
                     /gene="yihS"
                     /locus_tag="ACHOTW_001097"
     CDS             17257..18498
                     /gene="yihS"
                     /locus_tag="ACHOTW_001097"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFP9843251.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            18565..19467
                     /gene="yihR"
                     /locus_tag="ACHOTW_001098"
     CDS             18565..19467
                     /gene="yihR"
                     /locus_tag="ACHOTW_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFP9843252.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            19528..20403
                     /locus_tag="ACHOTW_001099"
     CDS             19528..20403
                     /locus_tag="ACHOTW_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9843253.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            20511..22547
                     /gene="yihQ"
                     /locus_tag="ACHOTW_001100"
     CDS             20511..22547
                     /gene="yihQ"
                     /locus_tag="ACHOTW_001100"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFP9843254.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            22593..23978
                     /gene="yihP"
                     /locus_tag="ACHOTW_001101"
     CDS             22593..23978
                     /gene="yihP"
                     /locus_tag="ACHOTW_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9843255.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            24021..25424
                     /gene="yihO"
                     /locus_tag="ACHOTW_001102"
     CDS             24021..25424
                     /gene="yihO"
                     /locus_tag="ACHOTW_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9843256.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            25491..26183
                     /gene="ompL"
                     /locus_tag="ACHOTW_001103"
     CDS             25491..26183
                     /gene="ompL"
                     /locus_tag="ACHOTW_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFP9843257.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(26274..27539)
                     /gene="yihN"
                     /locus_tag="ACHOTW_001104"
     CDS             complement(26274..27539)
                     /gene="yihN"
                     /locus_tag="ACHOTW_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9843258.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(27641..28621)
                     /gene="yihM"
                     /locus_tag="ACHOTW_001105"
     CDS             complement(27641..28621)
                     /gene="yihM"
                     /locus_tag="ACHOTW_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP9843259.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(28629..29339)
                     /gene="yihL"
                     /locus_tag="ACHOTW_001106"
     CDS             complement(28629..29339)
                     /gene="yihL"
                     /locus_tag="ACHOTW_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9843260.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(29556..31379)
                     /gene="typA"
                     /locus_tag="ACHOTW_001107"
     CDS             complement(29556..31379)
                     /gene="typA"
                     /locus_tag="ACHOTW_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP9843261.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            31752..33161
                     /gene="glnA"
                     /locus_tag="ACHOTW_001108"
     CDS             31752..33161
                     /gene="glnA"
                     /locus_tag="ACHOTW_001108"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP9843262.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            33335..34384
                     /gene="glnL"
                     /locus_tag="ACHOTW_001109"
     CDS             33335..34384
                     /gene="glnL"
                     /locus_tag="ACHOTW_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP9843263.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            34396..35805
                     /gene="glnG"
                     /locus_tag="ACHOTW_001110"
     CDS             34396..35805
                     /gene="glnG"
                     /locus_tag="ACHOTW_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP9843264.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            35917..36027
                     /gene="yshB"
                     /locus_tag="ACHOTW_001111"
     CDS             35917..36027
                     /gene="yshB"
                     /locus_tag="ACHOTW_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP9843265.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(36217..37590)
                     /gene="hemN"
                     /locus_tag="ACHOTW_001112"
     CDS             complement(36217..37590)
                     /gene="hemN"
                     /locus_tag="ACHOTW_001112"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP9843266.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(37779..38285)
                     /gene="yihI"
                     /locus_tag="ACHOTW_001113"
     CDS             complement(37779..38285)
                     /gene="yihI"
                     /locus_tag="ACHOTW_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP9843267.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(38404..38570)
                     /locus_tag="ACHOTW_001114"
                     /pseudo
     CDS             complement(38404..38570)
                     /locus_tag="ACHOTW_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38867..39499
                     /gene="yihA"
                     /locus_tag="ACHOTW_001115"
     CDS             38867..39499
                     /gene="yihA"
                     /locus_tag="ACHOTW_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9843268.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(39881..42667)
                     /gene="polA"
                     /locus_tag="ACHOTW_001116"
     CDS             complement(39881..42667)
                     /gene="polA"
                     /locus_tag="ACHOTW_001116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9843269.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            43031..43963
                     /gene="yihG"
                     /locus_tag="ACHOTW_001117"
     CDS             43031..43963
                     /gene="yihG"
                     /locus_tag="ACHOTW_001117"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP9843270.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(44004..45434)
                     /gene="yihF"
                     /locus_tag="ACHOTW_001118"
     CDS             complement(44004..45434)
                     /gene="yihF"
                     /locus_tag="ACHOTW_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9843271.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT        11201 a        11588 c        11048 g        11670 t
ORIGIN      
        1 gaaaacgcca ctacacgcat tttgtttata aataaaataa taaaattcac gaaatgaata
       61 agtcctgcaa tttaatatat atcgcaggac ttattttaat taattgctca aacgctgcgg
      121 atgggtataa accgttgccc gacccggttt acaaaaacct accagtgtca gattacagcg
      181 ctcagccact tctacagcaa gcgtggtcgc ggcagacacc gcaaacaaaa tctctacgcc
      241 gcacatcgcc gacttttgca ccatttcata actggcacga ctggaaacca gtaccgcacc
      301 ttgctgccag ctttcccctt cttgtgaccg gcggcctaac agtttgtcca gcgccacatg
      361 gcgacccacg tcttcatgcc cgccgaccag ttcgccagat ggcaacatcc aggcggcggc
      421 gtgagtacaa ccggtcagtt gtcccactgg ctgaaaatcg ttgagatgac gtaatgcatc
      481 atccagtttg ttgagatcaa acgtctgggt gaacggtagc ggctgcaccg gttttccgat
      541 gtcattaagt tgctccacgc cgcatacgcc acagcccgta cgtcccgcca gcgcccggcg
      601 gcgctccttc aaccccataa agcggcggct ggaaagctca atttgtactt caagaccatt
      661 acaggaagga acgacatcca tgccgaagat atcgcgcgga ctttctatta tcccctcgga
      721 aagcgaaaaa ccgagcgcaa agtactcaag gtctttgggc gacgccatca tcaccacatg
      781 cgaaatgccg ttgtagacca gcgcaacggg aacttcttcc gcgacctcat ctaaccgtgg
      841 gtgttgtaaa tcatcacgcc gccataactc aatttgacga acacctgtga tatttgtcac
      901 attttcgatt tctttttgct gtgttttttt cactttctct taaccagata agaacacaca
      961 caccaacatt atggtattct gttacaaacc cttcctggat ggagggaaat tgagccaatt
     1021 ctggaccttt gcggcccctt ccgcaaagaa aaataactcc cactccctgc acacgcagca
     1081 agcgaatgta aatgggacgt gacaatgtcg aaacaaggag caatccatgc aggtcagcag
     1141 aaggcagttc tttaagatct gcgctggcgg tatggcaggc accacggcag cggcactggg
     1201 ttttgcaccc agcgtagcac tcgcggaaac ccggcagtat aaactgctgc gcacccgcga
     1261 aacccgtaat acctgcacct attgttccgt aggctgtggg ctgttgatgt acagcctcgg
     1321 tgacggagca aaaaacgcca aagcatctat cttccatatc gaaggcgatc cggatcaccc
     1381 ggtcaaccgc ggtgcacttt gtccgaaagg cgctggcctg gtggatttca tccactccga
     1441 aagccgtctg aagtttccgg aataccgtgc gccaggttct gataaatggc agcaaatcag
     1501 ttgggaagag gcgtttgatc gcatcgccaa actgatgaaa gaagaccgcg atgctaacta
     1561 cattgcgcaa aacgccgaag gcgtgactgt taaccgctgg ctctccaccg ggatgctgtg
     1621 tgcttccgcg tcgagtaacg aaaccggcta tttaacgcaa aaattctccc gcgcgctggg
     1681 tatgctcgcg gtcgacaacc aggcgcgtgt ctgacacgga ccaacggtag caagtcttgc
     1741 tccaacattt ggtcgcggtg cgatgaccaa ccactgggtc gacatcaaga acgccaacct
     1801 cgtcgtggtg atgggcggta acgccgctga agctcacccg gtcgggttcc gctgggcgat
     1861 ggaagccaaa attcacaatg gcgcgaagct gattgtgatc gatcctcgct ttacgcgtac
     1921 ggctgcggtg gctgactact atgcccctat tcgttccggt actgacattg ctttcctgtc
     1981 aggcgtattg ctgtacctgc tgaacaatga aaaattcaac cgcgaataca ctgaagccta
     2041 taccaacgcc agcctgatcg tgcgtgagga ttacggcttt gaagatggcc tgttcaccgg
     2101 ctacgacgcg gaaaaacgca agtacgataa atcctcctgg acttatgaac tggacgaaaa
     2161 cggcttcgcc aaacgcgata ccacgctgca acatccgcgc tgcgtgtgga acttgctgaa
     2221 acagcacgtt tcccgctaca cgccagatgt ggttgaaaac atctgtggta cgccaaaaga
     2281 cgcgttcctg aaagtctgcg aatacatcgc agaaaccagt gctcacgata aaactgcctc
     2341 gttcctgtat gccctcggct ggacgcaaca ctccgttggt gcgcaaaaca ttcgtacgat
     2401 ggcgatgatc cagctgctgc tcggtaacat ggggatggct ggcggcggcg ttaacgccct
     2461 gcgcggtcac tccaatattc aggggctgac ggacctgggg ctgctgtcgc agagcctgcc
     2521 aggttacatg acgctgccaa gcgagaagca gaccgatctg caaacctacc ttaccgccaa
     2581 cacgccaaaa ccgctgctgg aaggccagga aaactactgg ggcaactacc cgaaattctt
     2641 cgtctctatg atgaaggcct tctttggtga taaagcgacg gcagaaaata gctggggctt
     2701 tgactggttg ccgaagtggg ataaaggcta cgacgtcctg caatacttcg agatgatgaa
     2761 agaaggcaag gtcaatggct atatctgcca gggctttaac cctgttgcct cattcccgaa
     2821 caaaaacaaa gtgatcagtt gtctgtcgaa actgaagttc ctcgtcacca tcgacccgct
     2881 gaacactgaa acctctaact tctggcagaa ccacggtgag ctgaacgaag ttgactcgtc
     2941 gaagatccag accgaagtgt tccgtctgcc atcgacctgc ttcgcagaag agaacggttc
     3001 aatcgttaac tcaggtcgct ggttgcagtg gcactggaaa ggtgcggacg ccccggggat
     3061 tgcgctgact gatggcgaga tcctctccgg tatcttcctg cgcttgcgca agatgtatgc
     3121 cgaacagggt ggcgcgaacc cggaccaggt gctgaacatg acctggaact acgccattcc
     3181 gcatgagccg aagtcggaag aagtagcgat ggagagcaac ggtaaggcgc tggccgatat
     3241 taccgatccg gcaaccggtg cggttatcgt caagaaaggc caacaactta gctcgtttgc
     3301 ccaactgcgc gatgacggta caacttcctg tggttgctgg attttcgccg gtagctggac
     3361 gccggaaggc aaccagatgg cacgccgtga taacgccgat ccgtctggcc tcggtaacac
     3421 gctgggttgg gcatgggcat ggccgcttaa ccgccgcatt ctgtataacc gcgcctccgc
     3481 agatccgcag ggtaacccgt gggatccgaa gcgtcagttg ctgaaatggg acggcactaa
     3541 gtggaccggc tgggatattc cggactacag cgcagcgcct ccgggtagcg gcgtcgggcc
     3601 gtttatcatg cagcaggaag gcatggggcg tctgtttgcc ctcgataaga tggcggaagg
     3661 tccgttcccg gaacactacg agccgtttga aacgccgctg ggaactaacc cgctgcatcc
     3721 aaacgttatc tcgaacccgg ctgcacgtat ctttaaagac gacgccgaag cattgggtaa
     3781 agccgataag ttcccgtatg tcggaaccac ctatcgtctg accgagcact tccactactg
     3841 gaccaaacac gcgctgttga acgcgatttt gcaaccagag cagtttgtgg aaatcgggga
     3901 gtcgctggcg aataaacttg gcattgccca gggcgatacc gtgaaagtct cctccaaccg
     3961 tggctatatc aaagccaaag cggtggtgac caaacgtatt cgcacgctga aagcgaacgg
     4021 caaagatatc gataccatcg gtattcctat tcactggggc tatgaaggtg ttgcgaaaaa
     4081 aggctttatt gccaatacgt tgacgccatt cgtcggtgat gcgaacacgc agacgccgga
     4141 gtttaagtcc ttccttgtga atgtggaaaa ggtgtaacgg agacgacata tggcttatca
     4201 atctcaagat atcattcgtc gttccgcgac taacggtctg acccccgcgc ctcaggcgcg
     4261 ggacttccag gaagaagtgg cgaaactcat cgacgtcacc acctgtatcg gctgtaaagc
     4321 ctgtcaggtg gcgtgttcag agtggaacga cattcgcgat accgtcggca ataacattgg
     4381 ggtgtacgac aaccccaatg atttaagcgc caaatcgtgg acggtgatgc gcttctcgga
     4441 agtggagcag aacgacaaac tggaatggct gatccgcaaa gacggctgta tgcactgttc
     4501 cgatccaggc tgtctgaaag cgtgcccggc ggaaggtgca attattcagt atgccaacgg
     4561 tatcgtcgac ttccagtccg agcagtgcat tggctgcggt tattgcattg cgggctgtcc
     4621 gttcgacatt ccgcgcctca acccggaaga caaccgcgtc tacaaatgta cgctgtgcgt
     4681 tgaccgcgtg gtggttgggc aagaaccagc ctgcgtgaag acctgcccaa cgggcgcgat
     4741 tcacttcggt acgaaagagt cgatgaaaac gctggcgagc gagcgcgtgg cggagctgaa
     4801 aacccgcggt tacgacaatg cgggcctgta cgatccggca ggcgtcggtg gtacacacgt
     4861 tatgtacgtg ctgcaccatg ctgacaagcc aaatctgtat catggcttgc cggagaaccc
     4921 ggaaatcagc gaaaccgtga aattctggaa aggcatctgg aaaccgctcg cagctgttgg
     4981 ctttgcggct accttcgcag ccagtatctt ccactacgtg ggtgtcggtc cgaaccatgc
     5041 ggatgaggaa gagaataatc tgcacgaaga gaaagacgag gagcgcaaat gaaacgacgt
     5101 gacaccatcg tgcgctacac cgcgccggaa cgtatcaacc actggatcac cgccttctgc
     5161 ttcatcctgg cggcggtgag cgggctgggc tttttgttcc cgtccttcaa ctggttgatg
     5221 caaatcatgg gcacaccgca gctggcgcga attctgcacc cgtttgtcgg cgtggttatg
     5281 tttgcctcgt tcatcatcat gtttttccgt tactggcatc acaacctaat caatcgggat
     5341 gatatctttt gggcgaagaa tattcgtaag atcgtcgtca acgaggaagt aggtgacacc
     5401 gggcgttata acttcggtca gaaatgcgtt ttctgggcgg cgattatttt cctggttctg
     5461 ctgctggtga gcggcgtgat tatctggcgt ccttattttg cgcctgcttt ctcaatcccg
     5521 gtgatccgat tcgcgttaat gctgcattca tttgccgcag tggcgttaat tgtggttatc
     5581 atggtgcata tctacgccgc cctttgggtg aaaggcacga ttaccgccat ggtggaagga
     5641 tgggttacca gcgcttgggc gaagaaacat cacccgcgct ggtaccgtga ggtccgcaag
     5701 acaacggaaa agaaagctga atgagtattc gcataatccc gcaagatgag ctgggttcga
     5761 gcgagaaacg tacggcggat atgattccgc cgttattgtt ccctcggctc aagaatttat
     5821 acaaccgccg cgccgagcgt ctgcgcgagc tggcagaaaa taatccgctg ggtgattacc
     5881 tgcgctttgc tgcgcttatc gcccacgccc aggaagtggt gctgtacgac catccgctgg
     5941 agatggatct gactgcgcgc attaaagaag ccagcgcaca aggcaagccc ccgctggata
     6001 ttcacgttct gccgcgtgat aagcactggc aaaagctgct gatggcgctg attgctgagc
     6061 tgaaacctga aatgagcggc ccggcgctgg cagtgattga gaatctggag aaggcatcga
     6121 ctcaggagct ggaagatatg gccagcgcac tgtttgcctc tgatttctcg tccgtcagca
     6181 gcgataaagc gccgtttatc tgggctgcac tgtcgctcta ctgggcgcag atggccaatc
     6241 tgatccccgg caaagcccgc gctgaatacg gcgagcaacg tcaatattgc ccggtttgtg
     6301 gttctatgcc ggtgtccagc atggtgcaaa ttggcaccac tcaggggcta cgttacctgc
     6361 actgcaacct gtgtgaaact gaatggcacg ttgtgcgcgt aaaatgcagc aactgcgagc
     6421 agagcggcaa actgcattac tggtcgctgg atgacgaaca ggccgcgatt aaagccgaaa
     6481 gctgcgatga ctgcggcact tacctgaaaa ttctctatca ggaaaaagaa ccgaaagttg
     6541 aagccgtggc agatgacctc gcctctctgg tactggacgc gcgaatggag caagaaggct
     6601 atgcccgcag ttccatcaac ccgttcctgt ttccgggtga aggggagtaa tctctgacgt
     6661 tttccgggtg gcgctggtcg cccggattac agctcatagg gtgtgacgac accatctctc
     6721 gtattccgcg ttaccacctc aagctatgcc gccagtgcct gttttgcctc aatttaccca
     6781 cccccacatc aataaggtta tgggtatgag actgatgata agcgtaagat tgcctgatgc
     6841 gccgcgctta ttaggcctac caataaactg caatacattg aatttatacg actttgtagg
     6901 ccgggtaagg cgttcacgcc gcatccggca tgaacaacgc gcacgttgtc agcaatctga
     6961 ttgatatcat tttttcccat taccattcct cgcgcagctt acgctggtat tcgacaccat
     7021 cttcctcaca cccttgccat atgccaaaag cactggttct tactgtttgc gattgattta
     7081 ccaagtattg atctaccgct tcccgtaata gctccgtacg aggaagatta cgctgcacct
     7141 caagatcatc aagttgctta atcacctcat tcgataaatc gagtataatt ctgcccatat
     7201 caatacctgc cagagagttc ataaatattt gaccaatatc atttgatatc tatggagaga
     7261 aaagtaccct tgtcgaatct ttaaagaaag ctcatttacg catcattttt tatttttgcg
     7321 aggcgttttc cagatatgat gtaacagttg cagttattat aagttcaagt aaaacagcct
     7381 tctattaata ccagaataag ggcatctgtt ttttatattc aagaatgaaa aatttttgtc
     7441 attccttatg ctccttacac tgatcatcaa caccaactac ctcccccttt tgcctgcctc
     7501 cctttgcttt acgcaccggg aattgctgac gttccagata ttgctctacc gcttcgcgta
     7561 aaattgcaga tcgtgaaacc ttacgccagc cccgaagctt gttcagccgt ttaattgcct
     7621 cttctgataa atgaagaaaa accgtattca tcgccattac caccacctta catatattac
     7681 tcattaatgt atgtgcgaaa attacgccga aaaattaatt aacgaaagcg gatttttgca
     7741 tgttgttttg tttttgcgag acgttttcca gaaataataa gagcgttgca gttgtttcat
     7801 ttgttactca aaaatggttt agttatcgcc caggatgagt tatgggtgct acgaatatat
     7861 tatattcagg gatatttatt ttttcattga atctttattt gcataatctg aatggtgtac
     7921 ccgaaaaacg tcagcatcct aacagcacca ggatttaagg tgaaattaat cttccatcac
     7981 agaccggtca gtactgccac caaccggtct ttacttgctc atcgttataa ttttgttggt
     8041 aacagttcgg gaaacaagga attccttccg gaacaccccc ggcatcatcc gatagcattg
     8101 acgtcaatca tcggctcgac ggtgagtttt tttaatcgca tcatagggtg gcaacgttca
     8161 gcacgatacg ggtcataaca aaactcctga gcagcggcac tatgccgctt tcacaggagt
     8221 tctgaatgcc ggtgctttct gtatggcttc gccatgcccc tcgcggctaa gcgcctgcac
     8281 acatctctct ttgtatttca tttttgcaat gcattggccg aattctgata atcctaatga
     8341 gttttactct tcagactccc atccctacgg tatacgagtt agattcatga atgaaatcga
     8401 catttttaaa aaacgaatct tgtaaaacat ccgtgttgat attaccttgc aacccaacat
     8461 taaatttgtt agagagatga agaactcgaa tatcaaaaag gtcttttaac atcattactt
     8521 cttcggctgt taatgaatcg caactaaaat atccctgttg atcaaaaagg ttttctattt
     8581 ctccatcatc ggcgaggcgt ttttcaaaga gtgtttgcaa catcgagttg ccaaggacaa
     8641 tggatatcgt gcgatctttc gcagatttac accccatcac aacggcaaca ttcgtatccc
     8701 cctcaggagt ttcatttatc gctgtcgcta taatggtggt caaacacgca aacggaactg
     8761 tatttctttc gtgatttaat ttatctacca gtttattcca tttatcaaaa gccgggcgat
     8821 atttgttgcg cacagaagta tccgactggt taaacaattc agccatgcgt ttttttatca
     8881 tctcgatatc cggcgaacca gaaagacttt gcgcataata cggtcctgat gatgttgcat
     8941 acattaaagc cgcgcccaca cctgtcgcca tgatgggtgc agccacggtt ttggcaaggg
     9001 cgacgctgcg atggctatgc gcttcacagg ctaaaggaac atactccact agctgcggta
     9061 attccttaca tgcctccctc atcattgcgg tatgctttac aaacaacggt tgttcactgc
     9121 ccaaaaaaac tttctgctta attgcactaa gaaaacgtaa atcaaaaatg attgtttttg
     9181 gtttattatt ttttggtaca ttacctatta tcccgctaag aaccttcctt gctgcatgga
     9241 gatttttttg cacagcacat aacacaattg actaagtctt ctctactgca ttattgattt
     9301 taattttaaa atccgcatga tatacgttga cgggcaatac aagtatgtct ggcgctacat
     9361 ctttggctga gtatttattg gtgatcgacg gccgtgaatc cacgagaaaa cttagctgca
     9421 aattatgctc tatattacca gctgtttttg tggtgtgatt aaacatagag atgccacctc
     9481 tcattctttc acacatatcc tcaacttttt cacttcgttc ttgtggcaat ttggctttaa
     9541 cgttaatttg atgattttta cccgtgcatt ccttaactac aagaggaatg ttatctaaag
     9601 tgccatacag gctaataaca tcaccaggat ttttatcatg taagtaagtt tgattaatac
     9661 tcgaagcatt cacaaagagg tcaatattat aactgtttat aacttcacct acatcaaatg
     9721 tgtagtgctt actctggagg ctgccatcct tatttaattg tatatcaatc aatatatccc
     9781 cggactttgt caccatatct ttgccataaa ccatttgcat agccatttcg aggagatttt
     9841 tatctaactg attgcgggcg ttgagtaagc gactaccaaa taatttatat actgttcccg
     9901 tcattgccag cgctgcgctt tctggtactt tgcgtttcca cagtgaggtt tctctatttt
     9961 taaacaggtc atattcatta ttactcgaca aaccatactt cagttcgtta acacaatgga
    10021 acagttgttc ctcaccttcc attttaaggc gatgtgaaaa atcatttaat agcgattgct
    10081 tcggtgattc attactcatt agttgttttt ctaaattaac aacagaataa ctttcttgtt
    10141 taatgagatt aatattggca accattacag cctccttccc acggtttaag agcaaacatg
    10201 ctacataatt atttataaca tccagagcag taaatgaatg atactgacaa tgatgccagc
    10261 ccattatctt tgcataatgt ttgttagcta tagattttta aagtatttat gagaaaacta
    10321 tggcaccaga gggggttttg actatcaaaa atcgctcaaa cctgaactaa gatgatgaat
    10381 taagcattat ctgcgataat tgctatacag ctctacaaat aatatttaaa tgctcctgaa
    10441 cagtgatgta gcaagacacg tatggagcca acgaagaata aaaatttata gagttcttat
    10501 tcaatataac cgaaatagcc aaggactaat ataaatgacc tgaatgagag aggcagcacc
    10561 gcccctctcc atcagctact cttcttcgtt cccgccctct tcatacgggc caaatggctt
    10621 cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt cgccaaagat
    10681 ttcgacctgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga gatcgccgct
    10741 taaggcacca aggtcggcta ccgcatgggg tttaccgcta atcggtttgc tgtagcggat
    10801 atgcgcatcc gccagaataa tcgttccgcc gaggtggcgt tcgcgcagca tcagccagat
    10861 aagcccccaa ccggtgagcg tcgccagtga gaataaactc ccggcaaaca gcgtatggtg
    10921 cggattctga ttgccggttt ctggcatggt agtgataaat ttttgcccgg tatattgctg
    10981 aatgcgcacg cccatttttt cactaagcgg gatatgttcg taccacgcct gttgcagctg
    11041 cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg gcgacgggct taatcatcaa
    11101 aaaatggcga atcggcgtgg tggttggcgt ggtgatttct ccctgattaa caaaccccag
    11161 cttggcgaaa aactccaccg cgtcttcacg ggcgctacag gtcacgcgct taacgccttc
    11221 ctgacgcgcc accgactcca gggtcatcgc catcagcgtg cctaaccctt tgtcctgcac
    11281 gtcgggatga acggccataa agcgaatgga cgcttcattg tcggcattaa tatacagtcg
    11341 gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg ccatcgcatc
    11401 ccacgcgtcg cgttccgaac cttttggttg atgcaggggc ttacgcaaca tttcccagcg
    11461 aaactgatag taacgctcta attcttcttc tgtttgtgga acccgaaggt gatacatagc
    11521 gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg caaccagaat
    11581 gtcacggggc catcattgac cagcgatacc tgcatatccg cagcgaagcg tcctgtttgc
    11641 gtgttcatct cttgctgacg gcagcgttcg acgaaatagt catataacgc ctctgcgcga
    11701 tccggtgatg cacctttgga gaaacttggg cgcatccccc gttcggtatc tgcggcgagg
    11761 gtaaactggg aaaccaccag cacactgccg cccgcctgtt gcacgttgag attcatcttg
    11821 ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg gtttgctttc
    11881 tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcacc aatttcgccc
    11941 gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa tgcaatcatg
    12001 gttcgtcgtc ttcttcttgt tcagctgctt gttttagttt gcggtattcc ccgagagtga
    12061 cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccaa acaaagagaa
    12121 tggggatcac cgccagcaca ccgtaaatga gctgatatga cgggaacatg gtgatataaa
    12181 gcgcgaaacc tttctttcct gcttcgaaca ggagtgcagc aacaaacgcg ccgacaatcg
    12241 cgtcgcggtt aggtacgcgg atggtaggaa caatgctgta cagcaaccag aaggagatcc
    12301 acgacaacag caacggaaaa atacgcagca cgttgtcgat gacagtattg agatcgctcg
    12361 cccagcgcag ggagagcaaa taggaactga tcgccagact ggcccctgcc agcagcggcc
    12421 ccagcgttaa aatcatccag tacacggcaa acgagtaaat tttgggtcgc gctcgtttac
    12481 tgcgccagat ggtattcaac gcgctatcga tggagtacat caacaataac gcggtgacga
    12541 tcagcccgca cgccccaacg gcggtcatct tgttggaatt ggcaacaaat tgttcgatat
    12601 accgctgaat aacatcgcca gtagcaggca gaaagttggc aaaaataaag tgacgtaact
    12661 gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca acggcaacca
    12721 gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt gtcatattgt
    12781 cctcatcaat gcgttgccag agtagtttta gccaggccca tagtggacgg gtacggtgcc
    12841 tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg ggatggtggt
    12901 tttatctttc accagaatac tggtaatgcc cagctgatta gctccttcta tattatcggc
    12961 gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca aaacatgctg
    13021 gtaaattcga gcttcaggtt tgcgcatccc cagatcttgc gacagataga tatggtcagc
    13081 agcatcacga atttccgggt attcttccgg ccagaaggtg gtatgcaggc ggttagtatt
    13141 ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg cgatcacttc
    13201 cgggcgcagc gcaacaaaca ccgcctgcca gccgtgagag aactgctcgt agcttagcgg
    13261 tagagccatc tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt ccccacgctc
    13321 atgctgatga aacgcctccc ccatatgaaa actcttctta agcgtagcca gcggaatacg
    13381 cgttaaatcg ctccaggttc ccagcacacg gttaaagtcg atatcgacaa tcacattacc
    13441 taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt aactgtagcg
    13501 ggaaaggatg ggtttggcta tgtggcaagg tatgaagcca ggtcaacagt cacaaaaatc
    13561 tacgcgtctt cctggggaag gatgatttca atttggctgt cttgtaagcg tgattttagc
    13621 gcagcgggcg gcagctcgtc ggtaaagagc gcggtgacct gtgccacgtt accaatttca
    13681 accgccgccg aagcatgata cttagtgtga tcggcaacca gcaggatatt tctcgcgtgc
    13741 gccatcatcg ttttcaccac gttagcttcg tttacatcaa actccatcaa cgcgccatcg
    13801 ctctcaatcg ccccaacgct tgttaccaga taatcagcac gaaaatcggc cacaaaggac
    13861 gccgctgaag ggccaatgat cccgctatta tgagagcgca acgtaccgcc gggtaccatc
    13921 acttcaaagc gcgggttgtg gtaaagaata tgcgccacac gcaggctgtt ggtgattatc
    13981 cgcaaatgat tatggttaag taacgcccgg gcaacatgct caacggtcgt accaatggtg
    14041 ataaatattg ttgaaccatc aggaatatag tctgccaccg cttcggcaat cgcttttttt
    14101 tcctcggttt gcgaaacctc acgctgctcg aacgccgtat taacgacgct ggaagcccga
    14161 cccgcgccac cgtgatggcg cgtaatcagg ccttgctcgc ttaatttacg aatatcccgg
    14221 cgcaccgtct gcgtggagac atccagcaga tttgccagct catcaatatt catataccca
    14281 cgctcggcga tcagcatgag gagttggtcg tgccgcgggt taccggttag ttcggtaagg
    14341 ctcatgaaaa ttcctcgaaa aaccatcacc ctggcatttt atacaaaaag tgacaaaaaa
    14401 gatcgggttt gatcacagtc agggatcccg gctcgtccac cgggacgcgt gcatttcgac
    14461 gccgctacac cgctggcgaa gcggactgac tccgctaaat ccccacctgt tgccagcacc
    14521 accgccaatg cgccgtgaaa aacatcaccc gcacctgtgg tatctaccac atcaactttg
    14581 aaggccggtt gatgttgacg cccgccattt tccagccagt cacagcctgc gctgccctgg
    14641 gtcacataga catgaccatt tgtgagcgtt tgtgcctgtt ttagcgcact ggccatctct
    14701 ttcacgcccg ttaagcgcgc cagacccggt tctgaaaagg ccgcatgatc gcttaatgcc
    14761 accagctcgc tgatatcctg cggcgtaata tcaccgtcca gaacggtcat cacgcccgcc
    14821 tgccgggcca gagtgaaggc ttttttagcg ccgtcgtgcc agcgtacatc tgccagcaca
    14881 acatcccact gagagaaatc aatttcctcc aaccactctg cgtcaggcag caggtccggg
    14941 ctggggtagt taatgattat ccgctcgcct ttggcatcca ccatgatggc ggattgcgac
    15001 gatttcgcct gtttataccg tttggtgtag cgggtgttaa cgccccagga ttccagctct
    15061 gccagcaggc tgttgccggt gtcgtcatca ccgacgcgcc caataaaatc aacctgcgct
    15121 cccagtcttg ccgccgcaac cgctgccgtc gccgctggcc cgccaccaac ttccgtataa
    15181 tttctcgcca cgtatttacc gctctccgtc ggtaaccctt ccacgtaata gatgcgatcc
    15241 atcacggtta tacctacaca agcaacacga atcatggtca ttccttaaac attttgggat
    15301 gcgttcattt taatttcacc aattgtttaa aaagtgacgt ctgtcaattt tttgactata
    15361 aattacaaat acgatcaaaa acagacaatc aaagcgtgtc ataaatgaca aaaagtgaca
    15421 tcactgtatt caggagaagg ttatggcagc aatcgcgttt atcggtttag gacaaatggg
    15481 ttcgccaatg gcgagcaatt tattgcagca agggcaccaa cttcgcgtct ttgatgtgaa
    15541 tgccgaggct gtgcggcatc tggtagacaa aggcgcgact cccgccgcca acccggcgca
    15601 ggccgctaaa gatgccgaat ttatcattac catgttgccg aatggcgatc tggtgcgcag
    15661 cgtgttgttc ggtgaaaacg gcgtttgcga aagcttatct accgatgcgc tggtcattga
    15721 tatgtcgacc atccatccgc tgcaaaccga taaattgatt gccgatatgc aagccaaagg
    15781 cttcaacatg atggatgttc cggtaggccg tacttctgca aatgccatta ccggtactct
    15841 gttactgctg gctggcggca ccgctgaaca agttgaacgt gccacgccga tcctgatggc
    15901 gatgggcagt gagttgatca acgcaggcgg tccgggcatg gggatccgcg ttaagctcat
    15961 caacaactat atgagcatcg cgctcaatgc gctttcggca gaagctgccg ttttgtgcga
    16021 agccctgaat cttcccttcg atgttgccgt caaagtgatg agcggtaccg ccgccggtaa
    16081 aggccacttc accacttcct ggccgaacaa agtcctcagc ggggatcttt ctcccgcctt
    16141 catgatcgat cttgcccata aggatcttgg catcgccctt gatgtcgcca accagctgca
    16201 tgtgccaatg ccgctggggg ccgcctcacg ggaggtttat agccaggcgc gcgcagcggg
    16261 tcgcggtcgc caggactggt ccgccattct ggaacaggtc cgtgtcagtg ccgggatgac
    16321 tgccaaagta aaaatgtaac gactggataa aggaataaat gaatgaataa gtacaccatc
    16381 aacgacatta cgcgcgcatc gggcggtttt gccatgctgg cggtcgatca gcgcgaagcc
    16441 atgcgcatga tgtttgccgc ggctggggca cccgctccgg tagccgatag cgttttaact
    16501 gatttcaaag ttaacgcggc gaagaccctc tcgccttatg cctcggcgat tctggtagat
    16561 caacaattct gttaccgcca ggtggttgag caaaacgcga ttgccaaaag ttgcgccatg
    16621 attgtcgccg ccgatgagtt cattcctggc aacggtattc cggtagatag cgtggtcatt
    16681 gaccgcaaaa tcaatccgct acagatcaaa caagacggtg gcaaagcctt aaaactgctg
    16741 gtgctgtggc gtagtgatga agatgcgcag caacgcctgg atatggtgaa agagtttaac
    16801 gaactgtgcc actcacacgg cctggtaagc attattgagc cagtcgtccg cccaccacgt
    16861 cgtggcgata aattcgatcg cgaacaagcg atcatcgatg ccgccaaaga gctgggcgac
    16921 agtggcgctg acctctacaa agttgaaatg ccgctttacg gtaaaggtcc gcaacaagag
    16981 cttctcagtg cttcacaacg tctgaatgac catatcaata tgccgtgggt gatcctctct
    17041 tccggtgtcg acgaaaaact gttcccgcgt gccgtacgcg tggcaatgac agcgggtgca
    17101 tcgggcttcc tggctggtcg tgctgtctgg gcatcggtcg tcggtttacc agacaacgag
    17161 ctgatgctgc gtgacgtttg cgcaccgaaa ttacaacaac ttggcgatat cgtcgacgaa
    17221 atgatggcta aacgccgtta aaatggggat acaaaaatga aatggtttaa cactttaagt
    17281 cacaaccgtt ggctggaaca ggaaaccgat cgcatctttg attttggtaa aaattccgta
    17341 gtaccgaccg gatttggctg gttaggcaat aaagggcaaa tcaaagaaga gatgggcacc
    17401 catctgtgga tcacagctcg tatgttgcac gtttattccg ttgctgcggc gatgggtcga
    17461 cctggcgctt actcgttggt tgatcacggt attaaagcca tgaacggcgc actgcgcgat
    17521 aaaaaatatg gcggctggta tgcctgtgtg aatgacgagg gcgtggtgga tgcctccaaa
    17581 cagggctatc aacatttctt tgctctgctg ggtgctgcca gcgccgtcac aacgggtcac
    17641 ccggaagcgc gcaagctgct cgattacacc attgaaatta tcgagaaata cttctggagc
    17701 gaagaagagc agatgtgcct ggagtcctgg gacgaagcct tcagcaaaac cgaagagtac
    17761 cgcggcggca atgccaatat gcacgcagtg gaagctttct taattgttta tgacgtaacg
    17821 catgacaaaa aatggctgga tcgcgcgatt cgcgtggctt ccgtgattat ccacgacgtc
    17881 gcaagaaata atcattatcg cgttaacgaa cacttcgata cccagtggaa tccgctgccg
    17941 gattacaaca aagataaccc ggcgcaccgc ttccgcgcgt tcggaggtac accaggccac
    18001 tggatcgaat ggggccgttt aatgctgcac atccacgcgg ccctggaagc ccgttgcgaa
    18061 caaccgccag catggctgct agaagatgcc aaaggtctgt ttaacgccac cgtgcgtgat
    18121 gcctgggcac ccgatggtgc ggacgggatt gtttataccg ttgactggga aggaaaaccg
    18181 gtggtccgcg aacgtgtgcg ttggcctatc gtcgaagcga tgggtacggc ctacgcgctc
    18241 tacaccgtca ccggcgatcg ccagtacgaa acctggtatc agacatggtg ggactactgc
    18301 atcaaatacc tgatggacta cgaaaatggt tcatggtggc aggagctgga tgcggacaat
    18361 aaggtcacca ccaaagtctg ggacggcaaa caggatattt atcacctgct gcattgcctg
    18421 gtgatcccgc gtatcccgtt agcgccaggc ctggctccgg cagttgcggc gggtctgctg
    18481 gatattaacg cgaaataact gactaaaaaa acgacatatc aggctgcacg ccgggtatgt
    18541 cgttattcaa taaaggtacc tcgtatgcaa atcaccaaca tgcattgcag tggacagacc
    18601 gtttcacttg ccgcggggga ttatcacgcg accattgtga ccgtcggtgc aggtctggct
    18661 gaattaacct ttcagggctg tcatttagtt attccacata aacccgaaga gatgccgctg
    18721 gcacatctgg gcaaagtcct tattccgtgg ccgaaccgga tcgccaacgg ctgttatcgc
    18781 tatcaggggc aggaatatca gttacccatt aatgaacatg gttcaaaagc cgctattcat
    18841 gggctgctcg cctggcgtga ctggcaaatc agcgaattaa ccgccaccag cgttaccctg
    18901 acagcttttc tgccgcccag ttacggctat ccgtttatgc tggcttcgca ggttgtttat
    18961 tcgctcaatg cacgcacggg tctttccgtt gagatcgcaa gtcagaatat tggtactgtg
    19021 accgcaccct atggtgtggg tattcatcct tatttaacct gcaacttaac atctgttgat
    19081 gaatatctgt tccagcttcc ggcaaaccag gtttatgccg tcgatgagca cgctaatccg
    19141 acaacgcttc accacgttga tgagctggat ttaaatttca cgcaggcgaa aaaaatagcc
    19201 gcgacaaaaa tcgaccacac ctttaaaacc gctaatgatc tgtgggaaat aaccattacc
    19261 catccacaac aggcgctttc ggttagcctt tgttccgatc aaccctgggt acaaatttat
    19321 agcggtgaaa aattacagcg tcagggactg gcagttgaac ctatgagttg cccgcctaac
    19381 gcctttaatt caggtctcga cttattatta ctggaaccag ggaaaacgca ccgcctgttt
    19441 tttaatattt acgggcaaca caattaattt tattaagaaa agaacgagat cattgtttag
    19501 agttgtattt acacaacagg aaatattatg gactttttta cgttattttc accggatggg
    19561 gtcatgctgg aaacaaacgt ggtatgtatc gttatcgcgt taatgtttct ttataccttt
    19621 gtggggatat gcgcgggatt tggcggtggt ttaaccacta tgccgctcgt cacgttaatg
    19681 ctccccgtaa aaatggcaac accactctcg gtaattgtcg gcaccgcgac cgccttgtac
    19741 gccacctggc tgtcacgtaa agaaacggat tggcgatcgg caatggtact gattattttc
    19801 tcgtttctgg gaatacccgt cggtttatat gcactgtcgt atttacccga ccattttatg
    19861 aaagtgggct taggcggatt ccttatcctg tactcctttt acagtatgtt tattccacgc
    19921 ctgccgattt atgaccgtag ctggatcgcc gcacctatcg gtgcagtagc aggcgcatta
    19981 ggcgcagctt tctcaaccaa cggaccaccg atggttatct acggtatgct gcgtaactta
    20041 ggcccggcgg catttcgtgg cactctgaac gctttcttca ccgcaaacaa catcgcggtg
    20101 atcggcgggc tggcgaccag cggaattttg actgtttcga tctttaaact ggtgctgttt
    20161 tgtattccca ccatgatcct cggttcactg gttggacaat atgtgcataa acgtatttca
    20221 gtcaaagtgt tccgtgtgat ggtcttttta ttgctgattg cctccggttc catgttgata
    20281 aaaggcgcgc tgggaattcc cgccgctgcc gcacttctac ctccgtttac cttgttagtt
    20341 atactgcaac tggtatttgg taaaaaagtg gcggcaatcc gtcaggctga agcaagtaaa
    20401 taatcactta tgcgggttta aatttatatc cgcacacatt ctttaattaa ttaaacctgt
    20461 aataacagga tggcatgaaa tattagcttt atactctatg gagattattt atggatacgc
    20521 cacgcccaca attaccagat tttgaatttc atcagaataa cgacagtttt accctacatt
    20581 ttcgacaacg tcttatttta acccatagca aagataatcc ttgtttatgg attggctcag
    20641 gtatagcgga catcgatatg ttccgcggca atttcagcat taaagataaa ctacaggaga
    20701 aaattgcgct taccgacgcc atcgtcagcc agtcaccgga tggttggtta atccatttca
    20761 gtcgtggttc tgacattagc gccacgctga atatctctgc cgacgatcag ggccgtttat
    20821 tgctggaact acaaaacgac aaccttaacc acaaccgcat ctggctgcgc cttgccgctc
    20881 aaccagagga ccatatctac ggctgcggcg aacagttttc ctacttcgat ctgcgcggca
    20941 aaccgttccc gctatggacc agtgaacaag gcgttggtcg caacaaacaa acctatgtca
    21001 cctggcaggc cgactgcaaa gaaaatgcgg gcggcgacta ttactggact ttcttcccac
    21061 agcctacgtt tgtcagcacg cagaagtatt attgccatgt tgataacagt tgctatatga
    21121 acttcgactt tagtgccccg gaataccatg aactggcgct gtgggaagac aaagcaacgc
    21181 tgcgttttga atgtgctgac acatacatct ccctgctgga aaaattaacc gccctgctgg
    21241 gacgccagcc agaactgccc gactggattt atgacggagt aacgctcggc attcagggcg
    21301 ggacggaagt gtgccagaag aaactggaca ccatgcgtaa cgcgggcgtg aaggtcaacg
    21361 gcatctgggc gcaggactgg tccggtattc gtatgacctc ttttggcaaa cgcgtgatgt
    21421 ggaactggaa gtggaacagc gaaaactacc cgcaactgga ttcgcgtatc aagcagtgga
    21481 acaaagaggg cgtgcagttc cttgcctata tcaacccgta tgttgccagt gataaagatc
    21541 tctgcgaaga ggcggcaaag cgcggttatc tggcaaaaga tgtcgccggt ggcgactatc
    21601 tggtggagtt tggcgagttc tacggcggcg ttgtcgatct caccaatccg gaagcctacg
    21661 cctggttcaa ggaagttatc aaaaagaaca tgattgaact cggctgcggc ggctggatgg
    21721 ctgacttcgg cgagtatctg cccaccgaca cgtacctgca taacggcatc agcgcggaaa
    21781 ttatgcataa cgcctggccc gcgctgtggg cgaaatgtaa ctacgaagcc cttgaagaaa
    21841 cgggcaagct cggcgagatc ctcttcttta tgcgcgccgg ttctaccggt agccagaaat
    21901 actccaccat gatgtgggcg ggtgaccaga acgtggactg gagtctggac gatggcctgg
    21961 cgtcggttgt cccggcggcg ctatctctgg caatgaccgg acatggcctg catcacagcg
    22021 acattggcgg ttataccacc ctgtttgaga tgaagcgcag caaagagctg ctgctgcgct
    22081 ggtgcgattt cagcgccttc acgccgatga tgcgcaccca cgaaggtaac cgtcctggcg
    22141 acaactggca gtttgacggc gacgcggaaa ccatcgccca tttcgcccgt atgaccaccg
    22201 tcttcaccac cctgaaacct tacctgaaag aggccgtcgc gctgaatgcg aagtccggcc
    22261 tgccggttat gcgcccgctg ttcctgcatt acgaagacga cgcgcagacc tattccctga
    22321 aatatcagta cctgttaggt cgcgacattc tggttgctcc ggtacacgaa gaaggtcgca
    22381 gcgactggac gctctatctg ccagaggata actgggtcca cgcctggacc ggagaaacct
    22441 tccacggtgg agaaataact gttgaagcgc ccatcggcaa gccgccggtc ttttatcgcg
    22501 ccgacagcga atgggctgca ctgttcgcct cgttaaaaaa catctaatca tgcccgcccg
    22561 tggggaaact cacgggcaaa tggagaataa cgatgagtca catcacaacg gaagatccgg
    22621 caactttgcg cctgcccttt aaagagaaac tctcttacgg tatcggcgat ctggcctcta
    22681 acatcctgct ggatattggt acgctttatc ttttgaagtt ttataccgac gttctggggc
    22741 tacctggcac ctatggcggc attatctttt tgatctcgaa attctttacc gcctttaccg
    22801 atatgggaac cggcatcatg ttggattccc ggcgtaagat tggcccgaaa ggtaagttcc
    22861 gccctttcat tttgtacgcg tcattcccgg tcaccttatt ggcaatcgct aactttatcg
    22921 gcacaccgtt cgatgtcact ggtaaaacgg tgatggccac tattctgttt atgctctacg
    22981 ggctgttttt cagcatgatg aactgctcct atggcgcgat ggtgcctgct attaccaaaa
    23041 accccaacga gcgcgcatca ctggcggcat ggcgtcaggg aggcgctacg ctgggcctgc
    23101 tgctgtgcac ggtgggattc gtgccggtta tgaatcttat cgaaggtaat cagcaacttg
    23161 gctatatctt cgccgccacg ctgttttcac tgttcggcct gctgtttatg tggatctgct
    23221 actcgggcgt gaaagagcgt tatgtcgaaa cccaaccagc caatccggcg caaaagcctg
    23281 gcctgctgca atctttccgc gcaattgccg gtaaccgccc actgttcatt ctgtgcattg
    23341 ccaacctctg cactttaggg gcgtttaacg tcaagctcgc catccaggtc tattacaccc
    23401 agtacgtact taacgatccc atcctgttgt catatatggg atttttcagc atgggctgta
    23461 ttttcatcgg tgtgttcctg atgcctggcg cagtcaggcg ttttggtaag aagaaggtct
    23521 atatcggcgg cctactgatt tgggtgctgg gcgatctgct caactatttc ttcagcggcg
    23581 gttcggtcag cttcgtggcg ttctcctgcc tggcattctt cggctcagcg tttgttaaca
    23641 gcctgaactg ggcgctggtt tccgacaccg tcgagtacgg cgagtggcgt accggcgttc
    23701 gttcggaagg aacggtctac accggcttca ccttctttcg caaagtgtct caggcgctgg
    23761 ctggtttctt ccccggctgg atgctgacgc aaatcggtta tgtgccgaac gtggcgcagg
    23821 ctgaccacac cattgaaggg ttgcgccagc tgatcttcat ctacccaagc gcactggcgg
    23881 tagtcaccat tgtggcgatg ggctgcttct acagcctgaa cgagaagatg tatgtccgca
    23941 ttgttgaaga aatagaagcc cgtaaacgca cggcgtaata ataataaaca atgccctgcg
    24001 gggcgttata aggagtgatt atgtctgacc ataatccact gacattaaaa ctgaatctgc
    24061 gggaaaaaat cgcctatggt atgggcgacg ttggttcgaa tttaatgctt agcatcggta
    24121 ctctgtatct cctcaaattt tataccgatg aattagggat gccagcttac tatggcggga
    24181 ttatctttct ggtggcgaag ttttttaccg cgtttaccga tatgctcacc ggatttttac
    24241 tcgattcgcg gaaaaatatt gggcctaaag gcaaatttcg tccctttatt ttatacgcag
    24301 cggttccggc ggcgttaatt gcaacgctcc agtttatcgc caccactttt agtttgccgg
    24361 ttaaaacgac gattgccacc gcgctgttta tgatgtttgg actttcatac agtctgatga
    24421 actgctcgta tggtgcgatg atcccggcca ttactaaaaa cccgaatgag cgcgcgcagc
    24481 tcgcggctta ccgccagggt ggtgcaacca tagggctatt gatttgtacc gtggcgttta
    24541 ttccgttgca gtcgcttttt tctgactcaa ccgtcggtta tgcctgtgcg gcactgatgt
    24601 tctccattgg tggctttatt tttatgatgc tgtgctacag aggcgtcaaa gagcattatg
    24661 tggacacagc accaaccgga cataaagcca gtattctcaa atctttttgc gcgatatttc
    24721 ggaatccgcc attgctggtt ttatgcattg ctaacttgtg taccctggcg gcatttaata
    24781 tcaaactggc gattcaggtc tattacaccc agtatgtgct gaatgatatt aatttattgt
    24841 cgtggatggg attcttcagt atgggatgca tcctcgtcgg cgtattactg gtgccattaa
    24901 ctgtaaaatg ttttggtaaa aaacaggttt atttagctgg catggtgcta tgggcggttg
    24961 gtgatatcct gaattatttc tggggaagta actctttcac tttcgtcatg ttctcttgta
    25021 tcgccttttt tggcacggcc tttgtaaaca gcctgaactg ggcactggtg ccagataccg
    25081 tagattacgg tgaatggaaa accggtattc gtgccgaagg ttctgtttat accggttata
    25141 ccttctttcg taaaatttct gccgcacttg ctggcttctt gccaggcatt atgctgacgc
    25201 aaatcggtta tgttcccaac atcgcgcaaa gtgatgcgac attacagggt ttgcgtcagc
    25261 tcatttttat ctggccttgt gcactggcaa ttattgctgc attgacgatg ggattctttt
    25321 acacactcaa tgaaaaacgg tttgcgttga ttattgagga aatcaaccaa cgcaaaaata
    25381 aagagatggc gacagaagaa aaaacggctt ccgtaacgtt ataaatgtca atagccgcta
    25441 tttccatcct ggtgggtggc ggcctcccta cgtttaaaaa tggacttatt atgaaaaata
    25501 ttaatgcaat aattttatta tcttctctga cctctgcctc agtattcgct ggcgcatacg
    25561 tcgaaaacag ggaggcatat aatctcgcct ccgatcaggg cgaggttatg ctacgtgtgg
    25621 gttataactt cgatatgggc gcgggtatta tgttaaccaa tacctacacc tttcagcgag
    25681 aagatgaact aaaacatgga tataacgaaa tcgaaggttg gtatccgtta tttaaaccaa
    25741 ccgataaatt aaccatccag cccggtggct taattaatga taagagtatc ggttcgggtg
    25801 gtgctgttta tctggatgtt aactacaaat ttactccgtg gtttaacctg acagtgcgca
    25861 accgttttaa ccataataac tacagctcaa ccgatttaaa cggtgatctg gataataatg
    25921 acagctacga gattggcaac tactggaact ttattattac cgataagttc tcatatacct
    25981 ttgagccgca ttatttttat aacattaatg atttcaatag cggcaatggc acgaagcatc
    26041 actgggaaat cactaacacc ttccgttacc gcattaacga gcactggctg ccctatattg
    26101 aattgcgttg gttagatcgc aacgtggaac cgtaccaccg cgagcaaaac cagattcgta
    26161 tcgggacgaa gtatttcttc tgatgtgaaa ctgccggatg cggcgtaaac gccttatccg
    26221 gcctacggtt gatacggttt tcgttggccg gattaagtac atccggccat taattaagct
    26281 tcagccgcca gcatctcttt cttacgttgg ctaatacgct taaccagcat tacggaaacc
    26341 accgcaccac agaatgcgaa gcaggccata atgccaaaca cgattttgta gccaataatc
    26401 cccggattta aatccagaat gtagccatac agactgaagc agaacatcgc cggggcgtaa
    26461 ccaataaagc tacccaacgc catcgccgcg cctgttttat tttcagcaat ttttgcttcg
    26521 ccgataggtg caaaaaatac ggctcgctgt gtaaagacta tcgcgccaaa gcccagcgta
    26581 catgccatcc ctaaatagac cggcatactt tcgtgcggca gcataatcaa cagtacgagc
    26641 gcagcggtac tgatgataaa ggtgtagcat agatatttac tcggcgattt caggatttta
    26701 tctgaaatca tgccaccaat cggtccacca atcattttca gacagtattg gttaatgatg
    26761 ccgtaagccc ccaccagcgc aacgggcaat agatagatgt ttttcaggaa tggaatgaag
    26821 aatgtcaggc cacagtaaac cgcgtaaacg aagaagacgt tgaaagcgat aagccagata
    26881 gttttgtctt tcagcaccga ggtcatactg gtgttttgcg atgccccgtc ttcttttttc
    26941 acctcaacgg acggtgcctc ttctttgtca ttcaggacaa agaaaatagt aatgcctacg
    27001 gcaatcacaa taagggaata gaaccagatg cctgctttaa aacctaataa gccactgcca
    27061 aaccaggtaa atactgccaa cgcagaaaat gccaccacgg tatcgacaat gccacgccct
    27121 gtttcgaaga agccaaacaa ccgaccttgt tgttcgctat tgcccaatcg acttaccgac
    27181 ttgagcaaga ccggccagtt catcatgtcg caagtaacgc caaatagcgc ccagacaaag
    27241 aggatgcccc aataccccgg cattgtcgtc aaataaacac ccagtaatcc tgtcgcaatg
    27301 agtgagaatg acatggtgta tctgcgcggt agtttatcgg caaaataaat agacagaaaa
    27361 aagcccactg tggtaacaaa tgagtttacc gacatagcat taccaatttg accattggtc
    27421 aaatggaaat attcctgcat cgggatataa aacgcatctt tcagcgacgg taatttataa
    27481 attgtaccgc cacacagtgt taatagacta aataacgccc atttcttttt cgtgagcata
    27541 acatccccta agcgatttta aaaatcactg ttttaaaggc aataagatac cgaccattct
    27601 tatggaatgg ttttattaac tgcgtatgag ttaaaacttg ttagtggttc agaagatggc
    27661 tcgcctcttg tttgatttgt tgtagtacgt tacgcacatg atttatctga ttaattgcat
    27721 cttctttttc tttacgtaac cgctcatcca gcaaatgctt ttctggcaat ggtgtttcac
    27781 tcatctggcg ataagggatc cacggattat catcttcgtc ttcaaagcgg aacgctggag
    27841 cataataatc aacctcttct tccaggccat atgccgtcac ctgcggttct tcatcgccga
    27901 ggcagataag gtgtgttaat aactctttga acggcatatc tccctgacct gaaatacagg
    27961 ctttatgacc caggccacct tgttctttaa cgatcaaggc atctttgata tggacctggg
    28021 taatatgcgg tgccatcgtt tttaaggcgt ctatgggatg ctcatttgca ttgatcatat
    28081 tcgcaaaatc aaacagtaag gaaagagact ccatctcact ttctttgacc agcgacacca
    28141 actcatgact ttttaaatct tcatgctgtt cgatcgtaaa agtcaggccg ctatcctgat
    28201 acgcttcccg cacataggca atgtcgttag cgatgatcga taatacgtcg cgcaaattac
    28261 cttcataacg tggataaaaa cgtacggacg atgccccagt tttcaacgcg atggcgacgg
    28321 cttcgtcaat agatgcctta tctgaggcgc tggtttcaat atgaatatca aggttcagac
    28381 ggcgggcttt atccccaaag gcagagagtt ctttatcgtc catattacca agagaaaaac
    28441 gctcgccatc cagaacatgg attttgacgc cacgtagatt attttcactt gcgatatcaa
    28501 gtaaatcagc aggcaacact ctttccagcc gcatatttaa atgaaaggca taagcatgta
    28561 aataaagagg aagagtgtcg acacgttgta gaatctttct tgcgttattt attgtcacca
    28621 taaaacacct acagattaat gatgaggtct acggcctcaa atcgccaata ttcaatatct
    28681 atctggacaa tattgccttg ctggtcggcg cgatgctttt caattaagat cgctggcata
    28741 cccgcagaac cgccaagata cttgctgata tcgcccggca ttctgacggg tttgaaaatc
    28801 aattttttta ctaccgtctc tttttggtag cacttttccc agactgcaga aaatgagtgg
    28861 ttttcaagtt gttcaataaa acccggagca acttcaggat taatatatgt ttcgtgatag
    28921 aaaactttat gtccttccag cgccccccag ccggtgatgc gatagagttc agttgatggt
    28981 gtcactgcaa tcagtggcgc gagcgtctcg ggaatatcgc tggtacggtt tttctcggta
    29041 aacccccagg aaggctctcg cccttgctca attgcggccc gctgaaagct cgccgacagc
    29101 tccggactgt aattaaatcg cggctgggtc acgaaccacc ccttacgatc cttacggaag
    29161 attttggatt ctgcctcgag gttcaacaac gcctggcgca gcgtcatacg tttaatgccc
    29221 agcagttctc ccagctctct ttctgaaggg agttttccac ccggcgtggt gatgccatct
    29281 ttcaaccacc gatccagttt ctcttttgca ttttctacgg tggattgatt ctccgccatt
    29341 gtttaatcct tttggtttaa accagatgaa gcatcttacg cttacgccaa aaaagaaagc
    29401 aattaacggc gtatgacttt gcagataatt gtgacaaagc tctctattga cgtaaccaca
    29461 gacttataag ggagatactc gcattggtgt gcgctcacga caggcaaaaa aaaacctgcc
    29521 atcgctggca ggttttttat gactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg
    29581 gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc
    29641 acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata
    29701 ggcggaacca gaacaacggc ttcgtcagta ccggaagcac gcatgttggt cagtttctta
    29761 ccggtcaggc agtttacagt caggtcgtta gagcggctat gaataccgat aatctgacct
    29821 tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc gatcctgcaa accgaacagc
    29881 gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc
    29941 acttcacccg gacgcacgtc gtcgtagtgg ctgaaggtgg agtacagcag acccgtaccg
    30001 gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag
    30061 tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg
    30121 cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca
    30181 tacggctctt gtttacgacc gtcgatttca cggaagataa ctttcggacg ggataccgcc
    30241 agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga
    30301 ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt
    30361 accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta
    30421 ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag
    30481 agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg
    30541 cccaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc
    30601 agaccgaggt ggcccagcac tttaccaact ttcgcgttgc gggttttgcc ttcgctatcg
    30661 atgatagtaa cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata
    30721 acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg
    30781 tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc
    30841 gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg
    30901 aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca
    30961 acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg
    31021 ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg
    31081 tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag
    31141 tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg
    31201 tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc
    31261 tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg
    31321 gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca
    31381 actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa
    31441 caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac
    31501 ggagcataaa aagggttatc caaaggtcat tgcaccaata tggtgcttaa tgtttccatt
    31561 gaagcactat attggtgcaa cattcacatc atggtgcagc ccttttgcac gatgttaagc
    31621 atgataacgc cttttagggg caatttaaaa gttggcacag ttttcgcttt atctttttta
    31681 cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgacaatga ccaatccagg
    31741 agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt
    31801 ttgttgattt gcgcttcacc gataccaaag gtaaagaaca gcacgtcact atccctgctc
    31861 atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg
    31921 gctggaaagg cattaacgaa tccgacatgg tgctgatgcc agacgcatcc accgcagtga
    31981 ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca
    32041 ccctgcaagg ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa gattacctgc
    32101 gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg
    32161 atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag
    32221 gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga
    32281 aaggcggtta cttcccggtt ccgccggtag actctgctca ggatattcgt tctgaaatgt
    32341 gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg
    32401 ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc
    32461 agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta
    32521 tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg tcgctgtcta
    32581 aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt
    32641 actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca
    32701 ccaactctta taagcgtctg gtcccgggct acgaagctcc ggtaatgctg gcttactctg
    32761 cacgcaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta
    32821 tcgaagtacg tttcccggat ccggcggcta acccgtacct gtgctttgct gccctgctga
    32881 tggccggtct tgatggcatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc
    32941 tgtatgacct gccgccggaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag
    33001 aagcactgaa cgagctggat ctggaccgcg agttcctgaa agccggtggc gtgttcacgg
    33061 atgaagcgat cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc gtgcgtatga
    33121 ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc
    33181 cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc
    33241 ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca
    33301 ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc
    33361 tgggcagatc ctcaactcgc tgattaacag tattttgtta atcgatgaca acctggcgat
    33421 ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg
    33481 tacgccgtta ccggaactgt tgagctactt ctcattaaat attgagctga tgcaagaaag
    33541 tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg acggtcgctc
    33601 gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc
    33661 gccgatggat aaccagcgcc gcttaagcca ggaacagcta caacatgctc aacaagttgc
    33721 tgcccgtgat ttagtgcgtg gcctggcaca tgagattaaa aatccgcttg gcggtttacg
    33781 tggcgcggcg cagctactca gcaaagcatt acctgacccg tcactgctgg aatataccaa
    33841 agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt tggggccgca
    33901 gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg tggtaacgct
    33961 ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgatc ccagcctgcc
    34021 ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc
    34081 gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca
    34141 actgacctta cacggcgaac gctatcggct ggcggcgcgg attgatgtgg aagataacgg
    34201 gccaggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga
    34261 aggtggcacc gggcttggct tatccatcgc tcgtaatttg attgatcagc attcaggcaa
    34321 aattgaattt accagttggc cagggcatac cgagttctcg gtttacctgc ctatcaggaa
    34381 ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc
    34441 cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacaac atttgagaac
    34501 ggcgcggagg tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc
    34561 cgtatgccgg gaatggacgg gctggcactg ctcaagcaga ttaaacagcg ccatccgatg
    34621 cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa
    34681 caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagccgt ggcgctggtt
    34741 gagcgcgcca tcagtcatta ccaggaacag cagcagccgc gtaatgttca gcttaacggc
    34801 ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg tattatcggt
    34861 cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa
    34921 ctggtcgctc atgccctgca tcgccacagt ccgcgagcca aagcgccatt tatcgctctg
    34981 aatatggcgg ctatcccgaa ggatttgatc gaatcagaac tgtttggtca cgagaaaggc
    35041 gcgtttaccg gcgcgaatac cattcgtcag gggcgttttg aacaggctga tggcggtaca
    35101 ttatttctcg atgaaattgg cgatatgccg ctggatgtgc agacgcgttt gctgcgcgtg
    35161 ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgg
    35221 attatcgctg ccactcacca gaatctcgaa cagcgggtgc aggaaggtaa gttccgtgag
    35281 gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg tgagcgtcgg
    35341 gaagatattc cccgcctggc gcgccatttt ttacaggttg ccgcgcgaga actgggcgta
    35401 gaagcgaagt tgctgcatcc ggaaaccgaa gccgcgttga cgcgtctggc gtggccaggc
    35461 aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg taatggctgc cggacaggaa
    35521 gtgttgattc aggatttgcc tggcgaactg tttgaatcaa cggttgcgga gagtacttcg
    35581 caaatgcaac cggacagttg ggcgacgctt cttgcgcagt gggcagacag agcgctacgt
    35641 tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg
    35701 accgcgttgc gacatacgca ggggcataaa caggaggcgg cgcggctact cggctggggc
    35761 cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac ggcttgtgtg
    35821 taaagattga ttattgagcg caaattgctg gtattttgcg ctttactgtt ccgataagtt
    35881 cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg
    35941 gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ctgttgctgc cgtgctgtgt
    36001 gccgcgatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc tctttgttca
    36061 tgccagatgc tgcgtgaacg ccttatccgg cctacaaaag caatattttc aacaggtttt
    36121 gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg atggcgcttt
    36181 cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgag agaactgctg
    36241 catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc ggatcagcaa
    36301 gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc catcttttgc
    36361 taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca aatcccactg
    36421 tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa tcacatcgcg
    36481 gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc cttgttcatc
    36541 cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt cgccaatcat
    36601 gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg tgtagccctg
    36661 gaaattacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat ccggacgggc
    36721 aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga tggtttcctg
    36781 caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt tgcgctgagc
    36841 agcaaaaatg gtcggcagat gcgcatagtt aaagacgctc agacggtcag ggtttagctc
    36901 cgccacgcgt ttcagggtaa aggcgaaact ttccggcgtc tgtttcggca ggccgtaaat
    36961 caggtcgatg ttggtggagg taaagccaat ctcacgcgca tggttaagca gcgcaaagat
    37021 aaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga agtcctgcac
    37081 gcccatactc aggcgattaa agccctcggc gcgtaaatga tcgagtacat ccagttcgat
    37141 ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga agttttcgcg
    37201 cagcagcttc atcaggcggc tgatttgcgc tttattaaga tacgtcggcg ttccgccgcc
    37261 ccagtgcagc tggctgacgt gacgcccggc gaacagcggt gcacgatgga cgatttcttg
    37321 ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa tcttattgca
    37381 gccgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag ataatggacg
    37441 ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt ctgaaaactc
    37501 cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct ggatcagggc
    37561 cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg tctctggcgg
    37621 ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag ccacttttgt
    37681 cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat aaccggaagg
    37741 gttatcagca gaacgggaag gcccacggcg gcaatccgtt agttgccccg cagcagacgc
    37801 atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata agagaggccg
    37861 agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga ttgctcttcg
    37921 gcactcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg ctcatccgtt
    37981 tctagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt cggtttgtgc
    38041 tgtttggtga ctttttcagt cacgcccaat ggaatagggg ttttactgcc aatacgtgga
    38101 tctttcggtg cgttctggcc tttgctgcct gacgtgttgt ttccgcccgc tgcgcggctg
    38161 cccggcgcat gaccacgacg ttttttctga cgcttgcggt cacgagcttc ctgattcagc
    38221 tcttcgcgcg ttttgcgacg cgcttttgca tgacctttgc tgcgtgatgg tgaagatggt
    38281 ttcataatgg ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca
    38341 gaaagcaagc aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa
    38401 cccttaacgg gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg
    38461 gaacaatccg tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga
    38521 caatcctctg tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt
    38581 agcgtcctcg ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc
    38641 atcagtattt gcacattttc agagttttat ataaaagcaa ttaactgatt tatatagctt
    38701 atttgacatg catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct
    38761 cacaaagcgt atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg
    38821 ttcaggtata atcgccgcaa agctttgact gacggagacg accgctttga ctaatttgaa
    38881 ttatcaacag acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac
    38941 cggaattgaa gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac
    39001 gctgactaac cagaaaagcc tggctcgtac ctcaaaaacc cctggacgca cccagcttat
    39061 caacctgttt gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc
    39121 ggaagtcccg gaagagatga agcgcaaatg gcagcgcgcg ctcggcgagt acctcgaaaa
    39181 acgtcagagc ctacaaggtc tggtagtgct aatggatatt cgccatccgc tgaaagattt
    39241 ggatcagcag atgattgagt gggcggtaga cagcaatatc gccgtgctgg tactgctgac
    39301 caaagcggac aaactggcaa gcggcgcacg taaagcgcaa ttgaatatgg tgcgtgaagc
    39361 ggtgctggcg tttaacggtg atgtgcaggt tgaaacgttt tcttcgctga agaaacaagg
    39421 cgtggacaag ctgcggcaga aactggatac ctggtttaac gagatgcagc ctgtagaaga
    39481 aacgcaggac ggcgaataat tttcttacct taatgcttat gccggatgtg gcgtatccgg
    39541 cccataaatt caatagatta caattctacc gttggcctga taaacatggt gcatcaggca
    39601 ttacggatct ttcctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct
    39661 aaaatattca gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac
    39721 ctctgtaccc tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt
    39781 aatttttttt cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta
    39841 tgttgcttac ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt
    39901 tttcgccact ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca
    39961 gttgatgaat ctgcttcgca acggcatcga catcatctct atgaacttca aataccagtt
    40021 catcgtgtac ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa
    40081 cggcaatcat cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg
    40141 ctgcacgttc agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca
    40201 gacggcgtcc gtccagcgtt tcaacgtagc cctgctcttt cgcctgagca cgggtgcgtt
    40261 ccatatactc cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt
    40321 ctttacgtgg aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac
    40381 caaagttgat cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac
    40441 caaacacttc tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca
    40501 agcctttgtc acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg
    40561 agacaatcac ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt
    40621 tacgcaccgg aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta
    40681 cagcctggtg ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct
    40741 tgtcggtgta agtcgatttc agcttcgcca gaccacgata ctccagaatc acttttggca
    40801 acggatagtc cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg
    40861 gcgttttctt cagcggttta atgccctgtt tttcaaacag aatggtttgt aactgcttgg
    40921 tggaagaaag gttaaattcc tcacctgcaa tttcatgcgc tttcttttcc agctcagcca
    40981 gacgaagggt gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt
    41041 tacgttcaat gcgtgaaagt actggcacca gcggcatttc gatattctcg aagacgttca
    41101 acggcccttt gtgtttttgc agatccggcc acattttcag atgcaactgc aaggtgacat
    41161 cagcatcttc ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaagtcagtt
    41221 gatttttgcc tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt
    41281 ccgcgaggct gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca
    41341 tggtatcaaa cgcaatccca cgcagctcaa tgccgtaatt cgccagaata ccgcgatcgt
    41401 atttcaggtt ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact
    41461 cgagtgcacg ctcgcgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa
    41521 tatatgccgc tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gctgagatgt
    41581 tatcaaggct gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa
    41641 tccacgcttt cagtgtttct tcatcaagga tggtgacgta gttgtcataa gaaatcaccg
    41701 ttgccgtcac ttctggtgct tcgtctgcaa cactggtttc ctgtggcttc gcggctggtt
    41761 ttgccccttt ggcctgtaac catttgcccg cttcgacatc agcagtccag cgtttgaact
    41821 catacttttt gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt
    41881 cacaggtcag ctccagttca acgtcggttt taatcgtcgc cagctggtat gagagataag
    41941 caacttcttt gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc
    42001 cagcaatttt ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg
    42061 cctgcgcggt tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca
    42121 tcagcgccag gaaatcgatg atcagttctg gcggcacgcc gtacttattc accacctctt
    42181 ccggtccgag gatggtattc gtcatggtgt tgataagcgt aatatttggc gtcaccagct
    42241 gcgccatatc tttatcgcca gtgctgatca gcaccggacg cccggctttt tcggcttcgc
    42301 gcgccagagt accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc
    42361 ccatcgcttt aaccatcgcg tgcaagggtt cgatttgtgc tcgcagatcg tccggcattg
    42421 gcgggcgatg tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt
    42481 caaagaccac cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga
    42541 ggacaccata catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt
    42601 gatatgcgcg ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct
    42661 gaaccataat gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggata
    42721 aaaacatgag tttttcaccg cttttcgtga aaatcttttg tagatcttct ggatcgctcg
    42781 caaatcgtca tgtggataac tttgtgcata aattatttac atcgctttta atttattatc
    42841 atgcttttta cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc
    42901 ctaataattg agataatggg attttttcta atgtggatag atatgaatta tttttctcct
    42961 taaggatcat ccgttatttg ggtcgttttc tgctaaaatc tgcgccttgc gcttctttag
    43021 cgcactgtct atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat
    43081 aacccttttg ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat
    43141 catcattgcc gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc
    43201 gcgtttttgt gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct
    43261 gaacccacac ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta
    43321 tctgcttatt tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg
    43381 taagcacatt ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct
    43441 tggcctggcg tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt
    43501 attacgtcat ccggagcgac gtggtaaaga tgttgaaacc actcggcgtt cttgtgaaaa
    43561 gtttcgcctg catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga
    43621 aaaacatcag caaacccact ccactttcca aaacttgttg ccaccaaagg cggcaggcat
    43681 tgcgatggcg ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgttgtg
    43741 ttatccggac aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat
    43801 tgtcgtacat gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga
    43861 caaaagcttt aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg
    43921 acttctgacg tcgttgatgt catcacagcg gcaggacaag taaattttgc tcgcctctga
    43981 atgagcagag gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct
    44041 tcttcgctca tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc
    44101 gaggcggtat tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg
    44161 ctgatttgtt tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta
    44221 agctgttttg cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat
    44281 ttgatatctt tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga
    44341 ttggcattta gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca
    44401 ctttgttgta ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca
    44461 acttcatcgt ttgctaattc agggtgtgca gcaagctgct tctgcatcgc aatgttatac
    44521 tggatgataa attggcgcac cgctgaggga tcaatagatt cagcaatcaa tgaccactca
    44581 ccactaccga gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca
    44641 ttgatataat ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt
    44701 tgggcaacgt cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca
    44761 ccaaccggaa atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc
    44821 gttactttgt tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc
    44881 aggttagaca atgaactact atcaccatta aaagtaaact ggccctcacc aaaagaaaac
    44941 tttccatact ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat
    45001 cgcaatgtcg cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt
    45061 tcattattca ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt
    45121 aacattgtga tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct
    45181 ggcttgatat tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa
    45241 ttaaaaaagc ctttgtcaaa attttgataa cttaacgtca tatcgtaggc atgttcacct
    45301 ttctttttag catcgttaaa atctttaata aatttttcga caccaggctg aatttgcgta
    45361 cctgtgtacc atgtgccacc accccagatt acggctaacg caacaataac acctgtagct
    45421 gacttacgta tcatataaat attccctgat aaaacagcaa aactttgaat atccacttat
    45481 gctgaaataa tgtccagcgg caggatg
//
LOCUS       DAZKIF010000019        45416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZKIF010000019 DAZKIF010000000
VERSION     DAZKIF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.019"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            105..326
                     /locus_tag="ACHOTW_001119"
     CDS             105..326
                     /locus_tag="ACHOTW_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9843272.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            323..1270
                     /locus_tag="ACHOTW_001120"
     CDS             323..1270
                     /locus_tag="ACHOTW_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9843273.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EEIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIAVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            1272..1448
                     /locus_tag="ACHOTW_001121"
     CDS             1272..1448
                     /locus_tag="ACHOTW_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843274.1"
                     /translation="MALTHRELCQIAYKFLKRNGFKVCFHDRFIAVTSTGEQPDAMGF
                     RNSASCLIGEMFSC"
     gene            1782..2138
                     /locus_tag="ACHOTW_001122"
     CDS             1782..2138
                     /locus_tag="ACHOTW_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843275.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEPIA
                     WECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLAYSDYA
                     EGWNDCREAMLQGDKS"
     gene            2135..2497
                     /locus_tag="ACHOTW_001123"
     CDS             2135..2497
                     /locus_tag="ACHOTW_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9843276.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPNDKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEP
                     ASKVTHWMPLPEPPQEVK"
     gene            2585..2827
                     /locus_tag="ACHOTW_001124"
     CDS             2585..2827
                     /locus_tag="ACHOTW_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9843277.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            2831..2965
                     /locus_tag="ACHOTW_001125"
     CDS             2831..2965
                     /locus_tag="ACHOTW_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843278.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            2984..3238
                     /gene="xisR"
                     /locus_tag="ACHOTW_001126"
     CDS             2984..3238
                     /gene="xisR"
                     /locus_tag="ACHOTW_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9843279.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            3272..4558
                     /locus_tag="ACHOTW_001127"
     CDS             3272..4558
                     /locus_tag="ACHOTW_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9843280.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            4579..5280
                     /gene="mlrA"
                     /locus_tag="ACHOTW_001128"
     CDS             4579..5280
                     /gene="mlrA"
                     /locus_tag="ACHOTW_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP9843281.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            5340..5447
                     /gene="yohO"
                     /locus_tag="ACHOTW_001129"
     CDS             5340..5447
                     /gene="yohO"
                     /locus_tag="ACHOTW_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP9843282.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(5428..6159)
                     /gene="yehW"
                     /locus_tag="ACHOTW_001130"
     CDS             complement(5428..6159)
                     /gene="yehW"
                     /locus_tag="ACHOTW_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP9843283.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(6164..7090)
                     /gene="yehX"
                     /locus_tag="ACHOTW_001131"
     CDS             complement(6164..7090)
                     /gene="yehX"
                     /locus_tag="ACHOTW_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP9843284.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(7083..8240)
                     /gene="yehY"
                     /locus_tag="ACHOTW_001132"
     CDS             complement(7083..8240)
                     /gene="yehY"
                     /locus_tag="ACHOTW_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP9843285.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(8247..9164)
                     /gene="osmF"
                     /locus_tag="ACHOTW_001133"
     CDS             complement(8247..9164)
                     /gene="osmF"
                     /locus_tag="ACHOTW_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP9843286.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(9416..11713)
                     /gene="bglX"
                     /locus_tag="ACHOTW_001134"
     CDS             complement(9416..11713)
                     /gene="bglX"
                     /locus_tag="ACHOTW_001134"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP9843287.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            11909..13624
                     /gene="dld"
                     /locus_tag="ACHOTW_001135"
     CDS             11909..13624
                     /gene="dld"
                     /locus_tag="ACHOTW_001135"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9843288.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(13662..14594)
                     /gene="pbpG"
                     /locus_tag="ACHOTW_001136"
     CDS             complement(13662..14594)
                     /gene="pbpG"
                     /locus_tag="ACHOTW_001136"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP9843289.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(14768..15355)
                     /gene="yohC"
                     /locus_tag="ACHOTW_001137"
     CDS             complement(14768..15355)
                     /gene="yohC"
                     /locus_tag="ACHOTW_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP9843290.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            15525..16103
                     /gene="yohD"
                     /locus_tag="ACHOTW_001138"
     CDS             15525..16103
                     /gene="yohD"
                     /locus_tag="ACHOTW_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9843291.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(16233..16994)
                     /gene="yohF"
                     /locus_tag="ACHOTW_001139"
     CDS             complement(16233..16994)
                     /gene="yohF"
                     /locus_tag="ACHOTW_001139"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9843292.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(17047..18483)
                     /gene="mdtQ"
                     /locus_tag="ACHOTW_001140"
     CDS             complement(17047..18483)
                     /gene="mdtQ"
                     /locus_tag="ACHOTW_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP9843293.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            18707..18790
                     /gene="yohP"
                     /locus_tag="ACHOTW_001141"
     CDS             18707..18790
                     /gene="yohP"
                     /locus_tag="ACHOTW_001141"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP9843294.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(19178..20128)
                     /gene="dusC"
                     /locus_tag="ACHOTW_001142"
     CDS             complement(19178..20128)
                     /gene="dusC"
                     /locus_tag="ACHOTW_001142"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP9843295.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
     gene            complement(20288..21481)
                     /locus_tag="ACHOTW_001143"
     CDS             complement(20288..21481)
                     /locus_tag="ACHOTW_001143"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFP9843296.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(21496..22140)
                     /gene="maiA"
                     /locus_tag="ACHOTW_001144"
     CDS             complement(21496..22140)
                     /gene="maiA"
                     /locus_tag="ACHOTW_001144"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFP9843297.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(22149..22850)
                     /locus_tag="ACHOTW_001145"
     CDS             complement(22149..22850)
                     /locus_tag="ACHOTW_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP9843298.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(22865..23893)
                     /gene="gtdA"
                     /locus_tag="ACHOTW_001146"
     CDS             complement(22865..23893)
                     /gene="gtdA"
                     /locus_tag="ACHOTW_001146"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFP9843299.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(23905..25263)
                     /locus_tag="ACHOTW_001147"
     CDS             complement(23905..25263)
                     /locus_tag="ACHOTW_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9843300.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            25390..26298
                     /locus_tag="ACHOTW_001148"
     CDS             25390..26298
                     /locus_tag="ACHOTW_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9843301.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            26429..26827
                     /gene="yohJ"
                     /locus_tag="ACHOTW_001149"
     CDS             26429..26827
                     /gene="yohJ"
                     /locus_tag="ACHOTW_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9843302.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            26824..27519
                     /gene="yohK"
                     /locus_tag="ACHOTW_001150"
     CDS             26824..27519
                     /gene="yohK"
                     /locus_tag="ACHOTW_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP9843303.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            27649..28533
                     /gene="cdd"
                     /locus_tag="ACHOTW_001151"
     CDS             27649..28533
                     /gene="cdd"
                     /locus_tag="ACHOTW_001151"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9843304.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            28683..29402
                     /gene="sanA"
                     /locus_tag="ACHOTW_001152"
     CDS             28683..29402
                     /gene="sanA"
                     /locus_tag="ACHOTW_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP9843305.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            29405..29644
                     /gene="yeiS"
                     /locus_tag="ACHOTW_001153"
     CDS             29405..29644
                     /gene="yeiS"
                     /locus_tag="ACHOTW_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP9843306.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            29838..31076
                     /gene="preT"
                     /locus_tag="ACHOTW_001154"
     CDS             29838..31076
                     /gene="preT"
                     /locus_tag="ACHOTW_001154"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP9843307.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            31070..32305
                     /gene="preA"
                     /locus_tag="ACHOTW_001155"
     CDS             31070..32305
                     /gene="preA"
                     /locus_tag="ACHOTW_001155"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP9843308.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(32548..33558)
                     /gene="mglC"
                     /locus_tag="ACHOTW_001156"
     CDS             complement(32548..33558)
                     /gene="mglC"
                     /locus_tag="ACHOTW_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP9843309.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(33574..35094)
                     /gene="mglA"
                     /locus_tag="ACHOTW_001157"
     CDS             complement(33574..35094)
                     /gene="mglA"
                     /locus_tag="ACHOTW_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP9843310.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(35155..36153)
                     /gene="mglB"
                     /locus_tag="ACHOTW_001158"
     CDS             complement(35155..36153)
                     /gene="mglB"
                     /locus_tag="ACHOTW_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP9843311.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(36432..37472)
                     /gene="galS"
                     /locus_tag="ACHOTW_001159"
     CDS             complement(36432..37472)
                     /gene="galS"
                     /locus_tag="ACHOTW_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP9843312.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(37614..38771)
                     /gene="yeiB"
                     /locus_tag="ACHOTW_001160"
     CDS             complement(37614..38771)
                     /gene="yeiB"
                     /locus_tag="ACHOTW_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP9843313.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(38788..39456)
                     /gene="folE"
                     /locus_tag="ACHOTW_001161"
     CDS             complement(38788..39456)
                     /gene="folE"
                     /locus_tag="ACHOTW_001161"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP9843314.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            39714..40550
                     /gene="yeiG"
                     /locus_tag="ACHOTW_001162"
     CDS             39714..40550
                     /gene="yeiG"
                     /locus_tag="ACHOTW_001162"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9843315.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(40582..42561)
                     /gene="cirA"
                     /locus_tag="ACHOTW_001163"
     CDS             complement(40582..42561)
                     /gene="cirA"
                     /locus_tag="ACHOTW_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP9843316.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(42853..44322)
                     /gene="lysP"
                     /locus_tag="ACHOTW_001164"
     CDS             complement(42853..44322)
                     /gene="lysP"
                     /locus_tag="ACHOTW_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP9843317.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(44527..45408)
                     /gene="yieE"
                     /locus_tag="ACHOTW_001165"
     CDS             complement(44527..45408)
                     /gene="yieE"
                     /locus_tag="ACHOTW_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP9843318.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
BASE COUNT        11260 a        12024 c        11417 g        10715 t
ORIGIN      
        1 cgatgaacat gaaaagcaaa atcatcaggg agctacaggc tcctttttta ttgttcgcat
       61 tcaccctcaa gcgtattaac caacaattca gggattaatg gaagatggca gacatcattg
      121 attcagcatc agaaattgaa gaattacagc gcaatacagc aataaaaatg cgtcgcctga
      181 actaccagac tgtatccgca actcattgtt gtgagtgtgg cgatccgata gatgaacgaa
      241 gacgcctggc agttcagggt tgtcggactt gtgcaagttg ccaggaggat ctggagctta
      301 tcagtaaaca gagaggttcg aagtgagcga aattaattat caggcactgc gtgaaaaagc
      361 agagaaagca actaaaggaa gctacatcgt agggcataca tctgttaacc agcacggcaa
      421 tttaacagga gtttttgttt gccaaaaatg gaaaggagaa cccggtggtg tgattgcgga
      481 atgtcatgtt aactgcctgg ttgaaacaga tgttcaggct tatgcaaacg ctgaatttat
      541 tgctgccttt aatccaaatg ttgcgctggc gcttctggat gaacgggaaa gaaaccagca
      601 atacatcaaa cgccgcgacc aggagaacga ggagattgcg cttacggttg ggaagctgcg
      661 tgttgagctt gaagcagcaa aatcaaaact caacgagcag cgtgaatatt acgagggagt
      721 aatcgcggat ggaagtaagc gcatagcaga actggaaaaa caatgcgccg aatgggagcg
      781 aaaagcatta agcaactttg aagagtgtgc tgcgatggct gaacgtatcg aagagatgca
      841 gacaaaatct gcaccagatt cgtttggcat catcggtgaa aatattcgaa cacaggacaa
      901 tcgaataacg tcagatccca tgttttgtgt gtatcaaaag cgcgaaatcg ctgttgatgc
      961 tgattatgac catgaccgga ttgtctgggt tgacgaagat ggcaatgaag ccaataaacg
     1021 ccatagtcgt cgtctcgagc tacttcatga aaactttcga gagccaccag aaaaatggcg
     1081 gcgcgttgct gtgaaagata ttgatgaatt cgttacctgc tgtttcaccg aacagggttg
     1141 taaagactac ctggcagtca atggtcacaa tcttcgcttg ccatttatat atgtaaaaag
     1201 cggtttcagg aacgctgaat atatcggcat aagaaactgg cttgctggca ttcgcatcaa
     1261 aggagagtga tatggcgtta acacaccgcg aactctgtca gattgcgtac aagttcctta
     1321 agcgcaacgg tttcaaggtt tgttttcatg accgctttat agctgtaacc agtaccggag
     1381 aacagccaga tgctatggga ttcagaaatt cagcatcatg cctgattggc gaaatgttct
     1441 cgtgctgact tgttggcaga tagaaaaaag cgttttcgta aaaatccgtc tcttggaatg
     1501 ggcgactggc gattctttat tagtgagccg ggaattattt caattgagga tttaccacct
     1561 ggctggggat tacttcacgt tgttaacgga agagtacgga aagtacatgg gtggcccaag
     1621 ggtaattgct gttggggtaa tcctgacgat aagccattta ctggaaataa gcaggttgaa
     1681 tgcgattaca tgttgtctgc attaaggcgc atggagttga gagggcacct taatgaaata
     1741 tatgacggtg taattgttaa taagaaagaa ggaaacgcgg catgaccact attaccgaca
     1801 aagaactgat taaagaaatc aaagagcgca taggcagctt ggacgttcga gacaatattg
     1861 agcgccgtgc ttatgaaatt gcactggcat cgctggaagc agaaccgata gcgtgggaat
     1921 gcggtgaaaa cataatcctg tttaaccctg acacagttga agcatacgca aaacgtgcgg
     1981 aaatatcacc taaaccacta ttctccgcgc cgccagcgct ggtagtgcct gataagttgc
     2041 cgcgtgaata cagaaacggt tggcctcttg cgtatagtga ttatgctgaa ggctggaacg
     2101 actgccgcga agccatgctt cagggagata aatcatgatt aatcgtatca agctggagca
     2161 catcctcgaa tatgccaggc agcagaggca tattggtcag cattgtaaaa ttccaccagg
     2221 agatatggtt gaaatcatgg agattgccat gcgcaaggct ggcaactctc cggtaactcc
     2281 ggatggttgg ataagctgta gtgagcgaat gccgaacgat aaacagtatg tttggtgttg
     2341 ggggaagtct tacggctgga ctgagtgcga taccttcgaa gggtattacg attgttcgag
     2401 aaacaaatgg tgggcagtta ctgacaatgg ggaagaaccg gcatcgaaag taacccactg
     2461 gatgccgcta ccggagccgc cgcaggaggt gaaatgatga attggcctga agcattcacc
     2521 gcagttggag ttgcaatagc ggtggcattt attctgtatt cgcttttccg ctggggataa
     2581 aggaatgttc gctctgattc aacgtggtca gatatacacg gacagagccg gataccctgt
     2641 ggtgattact cgcagtactc agcactcagt gttctttcga cgcatggacg ggcgctccgg
     2701 acgggtacgc attggtgagt tcaacaacct gttcgaacat attgaccaac aggagtaccg
     2761 caaaattctg gcgggcactg agcaggaaat gcgcctgaaa aaattacgcg caatgcaacg
     2821 gaggtgatac atgcatacgg cttttgagtt ctgggttcgc aagacattcg gcaatcgcta
     2881 cgacctgacc cgtgatgtcg acggcttcta ctgccgtgaa gttgtgaaac gaatgtttga
     2941 cgtgtggtgc cactgccgtg gatgaaagtt ttatgaggtt ggcatgcaga caatcatcta
     3001 tcagataacc cccagcaaat ggtgtacgga gagagtcctt attgcatcaa cagggctaaa
     3061 gcccggcacc atcgagcggg ccagaagaaa gtcatggatg cagggaaaag aataccgcca
     3121 ttacgctgta gaaggtgatc ctgggcatta cagtgaatgc ctgtacaaca tcgaagaaat
     3181 tatgcgatgg atcgaaaacc agaaacaacc aggtgccaaa aatgcaagtt ccggttaacc
     3241 tgttaatgct cctggacgtc tgggaggttt aatgagtaac gcatcatacc cgacaggcgt
     3301 tgaaaaccat ggcggatcac tccgtatatg gtttcactat aatggcaaac gtgtcagaga
     3361 aaacctcggt gttcctgaca cagccaaaaa ccggaagatc gctggtgaac ttcgcacttc
     3421 cgtttgtttt gcaatcagaa tggggagttt cgactacgcc gcgcagttcc ctaattcccc
     3481 taacctgaaa cactttggtc tgggaaaaag agagataacc gttaaggcac tttcggaaaa
     3541 atggttggac cttaagaaaa ttgagatttg tgcgaatgca cttaaccgtt accagtcagt
     3601 aattaaaaac atgttaccaa tgttaggtga aaaaaaactg gtttcatcca taacaaaaga
     3661 ggatttactt ttcgtaagga gagatttgtt gaccggttac caaaagcttt ctaatggaaa
     3721 gacttcttcc ataaaagggc gctcagtggt cacggtaaac tactatatga caaccatagc
     3781 tggaatgttt caatttgcaa cagataatgg ttatacctca ggaaacccat ttaacggtct
     3841 ggctccctta aaaaagtcca aggtaaaacc agatcctctc acccgtgacg aatttattcg
     3901 ttttattgag gcttgccgtc atcaacaaac aaaaaacctg tggattctcg ctgtatacac
     3961 gggtattcgt cacggggagt tggtatcgct ggcatgggaa gatatagacc ttaaagcaag
     4021 gactataacc atccgtagaa attatacaaa acttggcgaa ttcactccac caaaaaccga
     4081 tgcaggcacc ggaaggacaa ttcatctggt tcaaccagct attgatgctc ttaaaagcca
     4141 ggcggaaatg accatgcttg gaaagcaaca ttctgtagag gtgaagcaga gggaatatgg
     4201 gagaactgct gtgcataaat gcacttttgt ttttagtcct caggtaacaa aacagcagca
     4261 gttgtccgga cctcactaca aggttgactc catcagggag tcatggacaa gtatcttaaa
     4321 acgcgcaggt ctgagacaca gaaaatcgta ccaatccagg catacttatg catgctggtc
     4381 acttgccgca ggagctaatc ctagttttat cgcaagccag atgggccaca caaacgcaca
     4441 aatggtattc aatgtttacg gagcatggat gaaagacaac aatcacgaac agatagaact
     4501 ccttaacaaa agactatctg aaagtgtccc atgtatgccc cataagaaag cagggtaaaa
     4561 taaaaacttg caaaatcaat tagtttaccc ttaatccctg tcacgttacg cgcgtggcag
     4621 aggcgttacg gattgctgaa accgcaacgg acagacggcg gtcatcggct gttcaacgat
     4681 gccgatattg accggatccg cgagatcaaa cgctggatcg acaacggcgt gcaggtcagc
     4741 aaagttaaaa tgctgctcag taatgaaaat gttgatgtgc agaacggctg gcgcgatcag
     4801 caagaaacat tactgaccta cctgcaaagc ggcaatctgc atagcctgcg aacgtggatc
     4861 aaagagcgcg gtcaggatta ccccgcccag acgctcacca cacatctgtt tattcctctg
     4921 cgccgacggc ttcagtacca acaaccgact ctccaggcgc tgctggcgat cctcgacggc
     4981 gtactgatca actacatcgc catttgtctg gcttcggcac gtaaaaaaca gggtaaagat
     5041 gcgctggtgg ttggctggaa tattcaggat accacccgtc tgtggctgga gggctggatt
     5101 gccagtcaac aaggatggcg cattgatgtc ctcgcccact cgctcaatca actacgccct
     5161 gaactattcg aaggccgtac attgctggtg tggtgcggtg aaaatcgaac ctccgcccaa
     5221 cagcagcaac tcaccagttg gcaagaacaa ggccatgata ttttcccact cggcatttaa
     5281 tgattcgtta acaaatgcgc tttactgtac aatcctttcg ttaacataag gagtgcatta
     5341 tgcgcatagc taaaattggg gtcatcgccc tgttcctgtt tatggcgtta ggcggaattg
     5401 gtggcgtcat gctcgcaggt tataccttta ttttgcgtgc tggctaagcg cctgcaccag
     5461 cctttcaaac aggcggtctg cgatgatcgc cgccagtgcc accagtaacg ccccctggat
     5521 cacatacgcg gtattaaatc cgctaagccc gatgatgatg ggcgtaccca gcgtgctggc
     5581 ccctaccgtt gaggcgatcg tcgccgtacc aatgttgata atcaccgagg ttcgcacgcc
     5641 cgccagaatc accggagccg ccagcggtag ctcgacctta cgcagtcgct gacctcgact
     5701 cattcccata cctttcgcaa cttctgtcac gctggcatca atcgctccca gcccggcaag
     5761 tgtcgcctgc aggacgggca gcacaccata aaggatcaag gcgataatcg ctggttgcag
     5821 accaaagcct atcaccggaa cagcaatcgc cagcactgcg acaggcggaa aagtctgccc
     5881 aacggcggca atagtttcca ccagtgggcg aaattccgcg ccccacgggc gagtgactgc
     5941 aattccggca ccagtgccaa tgatcaccgc aaacaaactc gaaattccca ccagccagaa
     6001 atgagccagt gccaaagctg caaaactttc ttgctgataa acgggtcgtg gcagttgtgg
     6061 gaacaaggca gcaaacagcg gctggctgta aggcagccag aaaatcagtg ccacaaacaa
     6121 agcaatgagc cagaacagcg gatcgcgcaa catcttcata cgcttacgcc tccaccagca
     6181 gatcctgaaa atgcagcgtg ccgctaggct ggccctgcgt gttcaccacc ggcagcacct
     6241 cgcatccccg cgcgacaaac agggagagcg catcgcgcag cgtcatctct tctgccagtg
     6301 cctcaccttc tgcccgttct tcgcgacgca cgtaatccgc cacactacgt aacgaaagca
     6361 ggcgcacacc cagttcacta cgtccaaaaa actggcggac aaaatcattc gccggacgag
     6421 tcagcatcgt cagcggattc ccctgctgca ccacttcacc gtgatccatc aataccagat
     6481 gttctgccag ccgtagcgcc tcatcaatat catgcgtgac cagcacaatg gtacgcccca
     6541 gcaaacggtg aatgcgcgtc atctcttgtt gcaacgcgcc gcgcgttacc gggtccagtg
     6601 cgccaaaagg ttcatccatc agtaagactt gcggatcggc agccagtgcg cgcgctactc
     6661 ccacacgttg ctgctgacca ccggaaagct gatgtggata acgctcacgt aaatttgact
     6721 ccagccccag tagcgccatt aattcgtcga tacgatcgtc aatcctcgcc cgcgaccatt
     6781 tttgtaattg cggcacggtg gcaatgtttt gtgccacgct ccagtgggga aacaggccaa
     6841 tagattgaat ggcatagccc atacggcggc gcaactccag tactggcagc gagcgaattt
     6901 cttctccagc aaagcggagc tctccgctgt catgttccac caggcggtta atcattttca
     6961 gggtggtgga tttgccggag ccagatgtgc caatcagcac cgaaaaactc ccttcctgaa
     7021 aattgagatt gagatcgtta acggcttttt gtgcgccgaa cagtttgctg acatggctaa
     7081 attcaatcat tacgtttcac cttcaacagt gcgattagta aatcgaacag cgcatcgatc
     7141 agcaccgcca gaacaattac cgggatcacc cccagcaaca ctaaatcaat ggcgctgctt
     7201 agcagcccct ggaaaaccag cgcaccaaaa ccgcctgcgc cgattaacgc cgcaatcacc
     7261 gccatgccta cagtttgcac catcaccacc cgcaggctgc gcagaaatac cggtaacgcc
     7321 agcggtaact gaacatgcag gaatcgctgc gccccgctca tgcccatcgc tctggcgctc
     7381 tccagcacat cgcgcgggat ctggttcaag ccgactacca cgccgcgcac cagcggtagc
     7441 aaggcataga gcaccagcgc aatcagtgcg ggtgtcattc cggttcctgc tatgccaagc
     7501 ttccccagcc acggaaaggc cgtaaccagt ccggcaagcg gcgcaatcag caggccaaag
     7561 agcgccacgg aaggcacggt ctgaatgaca ttgagcagag aaaaaatcgc cccctgccgc
     7621 gcggtggaaa agtagcacca gatgcccaac ggcacaccaa tcactaacgc aggcagcacc
     7681 gcaccaaaca gcaaggtcag atgttgtgcc agcgcgtcgt caaacacatc ctgacggttg
     7741 gcgtattctt tcattagtga gagatcgtta agcgtgccgg agtacagcaa ccacagcgga
     7801 ataatggcaa tctgcatatg caacaaccag cgccacagcg gatgcgtgga gattcggcgg
     7861 atggcatcgc tacaggccag caatgccagc gccgaagcca accagaaacc actgccgagg
     7921 ctggtacgcg ccagcgcact gccattttgc gccagttggg tcgccgcctt tccagctccc
     7981 cacaccagca atacgaagac gaattgcgcc agaatgagtg caaaaatgct gcctttttta
     8041 gcgggaataa aacaggctgt cagccaggcg caaccaacgc ccaccagcat ccagatcgtt
     8101 tgcggccaca gttgccagag atgacgccct tcacccgaga ctaaacgatt aggcgcgtag
     8161 ctgataaacg gcagcgctgc tgcgattgcc gtcagcaaca gcagcagcgc cagaacagga
     8221 ttaatacgta gataagtcac gggaaattac ttcgtccacc ctttttgttt caagtagtcg
     8281 gcagccactt ttttggcatc cagtccttcc actgcaatgc tggcattcag ttgctgcaat
     8341 gtttttgcat cgaggctggc gaagactggc tgtagccact gtgccatttg cggatactct
     8401 ttcagcaccg actcacgcac cactggtgca ggcgcgtaga taggttgcac gccttgcgga
     8461 tcgcttaagg tttgcagccc ctgcgccgcg accggaccgt cagtgccgta agccattgcg
     8521 gcattaacgc cagaagtttg ctgggcagcg gctttaatcg ttaccgccgt gtcgccgcca
     8581 gccagtgaca gtaactgatc ctgatcgagt ttaaagtcgt aggctttttc aaacgcgggt
     8641 aacgcatcgg cgcgttcgat aaactcagcc gaggctgcca gtttgaaggt gccgccctct
     8701 ttcagataac gcctcaggtc agcaagcgaa gtgagtttgt ttttctctgc cacatcctga
     8761 cgcacggcga tggtccaggt gttatttgca ggtgcgggcg tcagccagat taacttgttt
     8821 tgctctgcat cgagtttttt gactttctcg taaccttgct gcgcgttttt ccacgctgca
     8881 tcgttttcat ctttaaagaa aaacgcgcca ttgccggtat attccggata gatatccagt
     8941 tcaccggaag taatcgcccc gcgcaccaca ggagtcgttc caagttgcac tttattgacc
     9001 gttggtactc cgtggctttc gagtacttgc aaaatgatat tgccgagcag cgcgccttcg
     9061 gtatcgattt ttgaaccgac tttaacgggg gaagccgcgt gcagcggcag gctcacggct
     9121 gccaacaaaa ccagtgaacc tgcccagacc tttgagagtg gcatgatgct ttcctcattc
     9181 ttttactgtt gttttcagcg aattaagaga aaagcatagt tgataatggc gggattagcc
     9241 tgagcggacg gattcagttg cagaatcaga taaatactta agaggcatat tcggtccggc
     9301 gtttcctgcc ggatgcggcg cgagcgcctt aaccggccta caaagggcga aaattcagca
     9361 agttaagata tgaacatcag gcaaagggcg caaacgtcgc gcccttgcta aagcattaca
     9421 gcaactcaaa ctcgcctttc ttaacgcgtg cggaatcagt gccgataaag acattgaact
     9481 tgccaggctc ggcgtcatat ttcatctgtt gattccagaa cttcagcgcc tcgatatcaa
     9541 tcgggaagct gacggtctgg gtttcgcccg gcttcagggt gattttctca aagcctttca
     9601 gctgcttcac tggacggctc atggaagccg tcacatcctg caagtacatc tgcactaccg
     9661 tggccccttc gcgtttaccg gtgttcgtca cctgcacgct ggccgtcact ttgccgtcac
     9721 gcttcatggt cggcgcagaa agtttcacat cagagacggt gaaagtggta tagctcagac
     9781 catagccgaa cggataaagc gccccgttag cttcatcaaa ataacgcgaa gtgtatttgt
     9841 tcggcttgtc ggcattatac ggacgaccgg tgttcagatg gctgtagtac accgggatct
     9901 gcccgacaga acgcgggaag gacatcggca gcttaccgga cgggttgtaa tcgccaaaca
     9961 acacatcggc aattgcatta ccgccttcag tcccggcaaa ccaggtttcc agaatcgcat
    10021 cagcctgctg atcttctttc accagcgcca gcggacgccc gttcatcagc accagcacca
    10081 gcggtttgcc ggtggctttc agggcggcaa tcaaatcacg ctggctttgc agaatagtaa
    10141 tatcggtacg gctggaggcc tcgtgcgcca tcccctgtgc ttcaccgact acagccacca
    10201 ccacatcaga ttgtttcgcc gtctgcaccg cttcatcaat catctcttgc ggcgaacgcg
    10261 gatcgacttt gaccgcttct tcatactgat tcaggaaatc gataatgcct ttgtcactgg
    10321 taacgttcgc ccctttggca tacagcactt taccgttttc accgacggca tttttaatcc
    10381 cggtcagcac ggtcacggat tgatcggcaa cacctgccgc cgaccagctg cccatcacgt
    10441 cacgtttact gtccgccagt ggcccaacca ccgcaatggt ggctgatttt ttcagcggta
    10501 acgtttcgag acggtttttc agcaacacca ggctttcacg cgccacttca cgcgcttctt
    10561 tgcggtgcag gcggctttcg gcattggtat ccaccgggtc agactctttc ggaccgagat
    10621 ggctgtatgg gtcgttaaac aaccccatat catatttaac gttcagtaca tggcgggcag
    10681 catcgtccag ctctgccatc gtcactttac cggatttgat caacccaggc agatacttcg
    10741 agtagtactc gtcgctcatg ctcatgttga ttccggattt cagcgccacg cgcactgcat
    10801 cttccggatc tgccgccgtg ccatgtttaa tcagctcttt gattgcaccg tgatcggaaa
    10861 cggtgatgcc tttaaagccc cactggtcgc gcagaacatc tttcagcagc caggagtcgg
    10921 aggtggctgg cgtgccgttc agcgagttca gcgccaccat caccgcgccg ctgcctgcgt
    10981 ccagccccgc tttgtacggc ggcatataat cattaaacag gcgctgcgga ctcatatcga
    11041 cggtgttgta ctctttaccg ccttctaccg cgccgtatgc ggcaaagtgt ttgacgctgg
    11101 tcatcaccga gtaacgatct gccgggcttt taccctgcat cgcttccacc atggttttac
    11161 ccattattga ggtgagatac gtatcttcgc caaaaccttc ggaagcacgt ccccagcgcg
    11221 gatcgcgcga gacatcgacc atcggtgccc aggtcatatt caggccatca tctgccgctt
    11281 cataagcaga gacacgtccg accgttttta ctgcatcgag gttaaaagac gaggccagac
    11341 cgaggctaat cgggaacacc gtgcgctgac cgtgcagcac gtcgtaagca aagaaaagag
    11401 gaattttcag gcggcttaat tccatcacct gatcctgcat ggcgcggata tcctgacggg
    11461 ttacggtgtt gaaaatagcc ccaacctgac cgtctttgat catctcgcgg atcgcctctt
    11521 tcgggttatc cgggccgacg ctgattaagc gcagctgacc aattttctca tcaactgtca
    11581 ttttcttaag cagttcggtg acgaacgcat cccgcgcttc aggcgttaat ggatggttac
    11641 cgaataaatc atccgccagt gctggctgca gggccagact caccgcgatt cctactgaac
    11701 atagccattt catatggatt tttcttcctt tgttgcccga cgtggcagcg aaaatggtgc
    11761 aaaaaccgta gtttgccata agcatgatgg agagagaaaa agaatgctca gtttattgtc
    11821 tgaattttca aaatattcac tcgctgaatt gtcatacaag gcgctattct agtttgtgat
    11881 gttttttcgc caccacaagg agtggaaaat gtcttccatg acaacaactg ataataaagc
    11941 ctttttgaat gaacttgccc gtctggtcgg tcattcacac ctgctcaccg atcccgcaaa
    12001 aacggcccgc tatcgcaagg gcttccgttc tggtcatggc gacgcgctgg ctgtcgtttt
    12061 ccctggctca ctactggaat tgtggcgggt gctgaaagcc tgcgtcaccg ccgacaaaat
    12121 tattctgatg caggctgcca atacaggcct gaccgaagga tcgacgccaa acggtaacga
    12181 ctatgatcgc gatatcgtga tcatcagcac cctacgtctc gacaagctgc acgttctcgg
    12241 caagggcgaa caggtgctgg cctatccggg caccacgctt tattccctgg aaaaagccct
    12301 caaaccgctg ggacgcgaac cgcactcggt gattggttca tcgtgcattg gcgcatcggt
    12361 tatcggcggt atctgtaata actctggtgg ttcgctggtg cagcgtggcc cggcgtatac
    12421 cgaaatgtcg ttattcgcgc gtataaatga agatggcaaa ctgacgctgg tgaaccatct
    12481 ggggattgat ctgggcgaaa cgccggagca gatccttagc aagctggatg acgatcgcat
    12541 caaagatgac gatgtgcgtc acgatggtcg tcacgcccac gattatgact atgtccaccg
    12601 cgttcgtgat attgaagccg acacgcccgc acgttataac gccgatccgg atcggttatt
    12661 tgaatcttct ggttgcgccg ggaagctggc cgtctttgca gtacgtcttg ataccttcga
    12721 agcggaaaaa aatcagcagg tgttttatat cggcaccaac cagccggaag tgctgaccga
    12781 aatccgccgt catattctgg ctaacttcga aaatctgccg gttgccgggg aatatatgca
    12841 ccgggatatc tacgatattg cggaaaaata cggcaaagac accttcctga tgattgataa
    12901 gttaggcacc gacaagatgc cgttcttctt taatctcaag ggacgcaccg atgcgatgct
    12961 ggagaaagtg aaattcttcc gtccgcattt tactgaccgt gcgatgcaaa aattcggtca
    13021 cctgttcccc agccatttac cgccgcgcat gaaaaactgg cgcgataaat acgagcatca
    13081 tctgctgtta aaaatggcgg gcgatggcgt gggcgaagcc aaatcgtggc tggtggatta
    13141 tttcaaacag gccgaaggcg atttctttgt ctgtacgccg gaggaaggca gcaaagcgtt
    13201 tttacaccgt ttcgccgctg cgggcgcagc aattcgttat caggcggtgc attccgatga
    13261 agtcgaagac attctggcgc tggatatcgc tctgcggcgt aacgacaccg agtggtatga
    13321 gcatttaccg ccggagatcg acagccagct ggtgcacaag ctctattacg gccattttat
    13381 gtgctatgtc ttccatcagg attacatcgt taaaaaaggc gtggatgtgc atgcgttgaa
    13441 agaacagatg ctggagctgc tacagcagcg cggcgcgcaa taccctgccg aacataacgt
    13501 cggtcatttg tataaagcac cggagacatt gcagaagttt tatcgcgaga acgatccgac
    13561 caacagtatg aatccgggga tcggtaaaac cagtaagcgg aaaaactggc aggaagtgga
    13621 gtaaaaatta cggatggcag agtatcgcca tccgaattca cttaatcgtt ctgtgccgtc
    13681 tgccccgccg ccgccatttg ggcggctttt tgttttttat agcttaacgc tgctgccgga
    13741 acaggcatca ctttaccggt ttcaatccag gtacgcaggc ggctggcatc ggcaaaatgg
    13801 gtgtatttgc caaacgcatc catcactacc agcgccaccg gtttattatt gataaccgta
    13861 cgcatcacca gacaatggcc cgccgcattg gtaaagccgg ttttggttag ctggatattc
    13921 cagttatcgc gatacaccag atgattagta ttgcggaacg gcagcgtata cgtcgggtta
    13981 gagaaggttg ccatatcttc ccgggtagta cttaactgcc cgatcaacgg atattgtttg
    14041 ctggcaatga gcagtttggt taagtcacgg gcggttgaaa cgttatgcac cgacaatccg
    14101 gtaggttcaa caaagcgcgt gttgttcatt ccgagcgatt tcgctttcgc attcattgcc
    14161 ttaataaagg ctttgtaacc accgggataa tggtgcgcaa ggcttgccgc cgcgcggttt
    14221 tctgaagaca tcagcgccag caacagcata tctttacggc tgatttcgct attcagtcgt
    14281 acgcgcgaat agaccccttt catctccggc gtctggctga tatccacttt tagtttttca
    14341 tccagcggca gtcgtgcatc cagcacgacc atcgcagtca ttaatttgct gatagacgca
    14401 atcggacgca ccagatccgg gtggttcgaa tagatcactt tgttggtatt cagatcaaca
    14461 atcatcgcgc taccggaggc aatttccggt tgtgaagcgg tggtagcggc tgccgttttc
    14521 gcaacggcct gcggtgccaa aggcacagcc agcatcaggg ccaggctaaa taaagaaact
    14581 cgaaatttcg gcatgatgag cattcagata gtggttcacg cgcacgggtt gcgcaccgcc
    14641 ggagtaagga tttactgagg ctagcgacgc catcataacg agcaaaaagt gcgatcgtca
    14701 aaggagaatc gtgaggaaat gctgcattgc tgacatttac gccagcaatg caacgtcaaa
    14761 gaactttcta gaacaaacga taaccgtagc cccatagtat aacggtaagg gcgagcagca
    14821 cttccagtac cagcacgcca atcgccagcg tcgaactgga gaagctaagg ccttcctctt
    14881 tgttgatatt caggaagctc ggaataccaa ggtaaagtag gtaaccggtg taaaacagtg
    14941 ccaccgtgcc gaccagcgca cacaaccaga ccagcggata aagcgccacc agaccactta
    15001 aaaacagcgg agttgcaacg tagcccgcga agaccatgca gtgcgcaagt gacggacgct
    15061 gcggataatt acgcgccatc caccagatga cccgccccat caccgcgacc ccagcgagca
    15121 taacgccata aaacaggaca gccagcgcca gtccggtaaa ccaggataac ttcaggatag
    15181 tgccatcgcc aaaattccag ccaatctgtg tagtgccaat gaaggcgcaa atcaccggaa
    15241 tcgccgccat cagcaaaacg tggtgggtgt aatggtgaga aatcgtttcg ttttcgcgat
    15301 taatcacctg catttcacga tcgggatggg aaaacagtcc ccagacatgg ctcataccgc
    15361 ccccttgttg tgagttcatg aacctgacag ttcaagtata agtcagcttg tgattatttt
    15421 ttgttcgccc atgtaatttg caccgtctga tgtacgtcct ttttcagcgt gaatgattca
    15481 cagggtgtat gcttacaggc aaccaaaggg agacagactg gcctatggat ctcaatacac
    15541 tcatctcaca atatggttat gccgcgctgg ttatcggtag cctggcggaa ggtgaaaccg
    15601 tgactttgct gggaggcctt gcggcacatc aggggctatt aaagttcccg ctggtggtac
    15661 tttctgtagc gcttggcggc atgattggcg accaggtgct ctatctgtgc gggcggcggt
    15721 ttggcggtaa gctgttacgc cgtttctcta aacatcagga taaaattgag cgggcgcaga
    15781 aactgatcca acgccatccg tatctgtttg tcattggtac gcgctttatg tatggctttc
    15841 gggtgattgg cccgacgctg attggtgcca gccagctgcc gccgaaaatc tttctgccgc
    15901 ttaatattct cggcgcattt gcctgggcgt tgatttttac cactatcggt tacgctggtg
    15961 gtcaggtgat tgcgccgtgg ttgcacaatc tcgaccagca tttgaagcac tgggtctggt
    16021 tgattctggt cgtggttctg gtggtaggcg tgcgctggtg gctgaaacga cgcgggaaga
    16081 aaaagccgga taatcaggcg taaaaccatt gccctggata aggcgtgcgc gccgcatccg
    16141 acatccggtt caccatgcct gatgcggcgc ttgtgcgtct tatcatgcct accaaaattt
    16201 tacccattga aatatccaca cacctcactg cgctattctg ggttgaactg cggattcgcc
    16261 aacataaagc cgccatccac tatcaacgac tgcccggtgg tgtaatttgc gccctccgaa
    16321 caaagccacg ccaccaggct ggcaatctca tgcgttgcgc caaaacgccg cagaggaatc
    16381 gaaggctccg cgtcgggctt cacgtcgcta tcatccatgc cgttcattgg cgtagcgatc
    16441 gccccaggtg cgactgcgtt caccaaaatc ttatgcctga ccaactccag cgccattgct
    16501 ttggttaacc cgccgagcgc atgtttagcg gctgtgtagg cgctggcatc cggcagcggc
    16561 gtatgttcat gtaccgacgt aatgttgatg atgcgaccac cctgtccttg tttcaccatc
    16621 tggcgggcag caatttgcga gcataaaaat gcaccatcga catcaacggt aaaaatcttg
    16681 cgccactcat cgaaagccat atcaagaaac ggcactttgg tcattgcgcc cgcattattc
    16741 accagcacat caatacgccc cagccgttga ataagtttct ccagcgcctg cgcaccttct
    16801 ggcagattgc cgagatccag ttgcacgatc tccgcacgta cgccgtggct aactacctca
    16861 cgcgcggtat cttttgcccc ttcttcatct gagtgccagg taatgccaat atcaaaccct
    16921 tgctgcgcca gtaataacgc gcactctttg ccgatccccg aatcggaggc ggtaataatc
    16981 gctacctgtg ccatcgagtt ctccacttaa cgctgaataa acgttaagta tagaagtcac
    17041 atatcatcag cgtttgtaac ccccgcccaa cgcaccagtg agttgaatgg aggcatccag
    17101 ccactgccct tgcaataaca ggccattggc ccgctcacgc agcgcgggga ttctggcttc
    17161 gctgacgcgg gaaccagcaa tgatgcccgc gttaaagcgg gcctgcgcaa gacctaccac
    17221 acgcaaggca tcgcgctcaa tttgcgcctg atgctggttt ttctccgcca gtgtctgaac
    17281 ctgactggct gcccgcgcca cgtcattcac cgcttcaacc accgctttgt tgtagctggc
    17341 gatagacaag ttgctttcgg cttttgcgat atcgagattg gcgttaagac gaccactatc
    17401 gaaaatgggt agcgtcaggc ctgccgtaac gcccatttgc tgcgcggaat gacggaacag
    17461 atcgcttaag tgcaacgcat cctgttgcag gaaggccatc aggttgatgt caggataaaa
    17521 tgccgctttt gccgcatcaa tggtgcttag cgatgactca acgtaccagt gcgccgcctg
    17581 caaatctgcc cgccgggcca gtaaggagta ccccagttca tcaggaagct ggcttgccac
    17641 tttcggcaac gccaccggtt taagcgtcaa tgacttagtc tggtgatttg tgagtgcaat
    17701 taaccgtgcc tcaataattt tcattttccc cgcgacatcg ttgagctgct gccgggtttt
    17761 gctggcatta atatcggttt ccacaccttc aactgaagaa gtaatcccgt tctgatatag
    17821 ctggcgatcg gtcgcgataa tggtgttctg ctctttttcg atttgctgca agaccgtgtt
    17881 taacgccgcc tgggtttgcc actcccagta caggcgggct acgctgccag ccagcaattg
    17941 gcgggtttgc tcgcgttccg ccgcccgtgc tttaaccgta cccaggcggg cagtaacctc
    18001 cgcccgattc tttccccaga tatcgagatg ccagcccgcc gttaagccaa aagttccgtt
    18061 ggtgtaccac ggaccggtcg tacctgcggc cggatcgttc agagcaaacg gccccattag
    18121 cccttctgcc gacatttttt gccgctccat atccgcagaa aagtcgatct gcggaccatc
    18181 ctgagtagca actgccttcg cctgggcttc agctaactga atgcgctgtt cagccacctg
    18241 catatccggt gcgttctgta gtgcattgtt aattaaggaa gtgagttgat tatcgtgata
    18301 ctccagccac cattggctgt ctggccaacc atttttcagc gccgtgggta atgcggtgtc
    18361 aacttgtgca gcgggcgttt gctggcttaa cgcctggcgg gtttcatgca taggcgcaca
    18421 cccggccagc atcagtaaca gcggaaaaca ggcgatggct ggataaaagg aatcacgatt
    18481 catgggggaa taatcaggta agaaaaggtg cgcggagatt accgtgtgtt gcgatatatt
    18541 ttttagtttc gcgtggcaat acatcagtag caataaaacg acatatccag aaaaatatac
    18601 actaagtgaa tgatatcttc cgatttatct taatcgttta tggataacgg caaagggctt
    18661 cgttttttcc tatacttatt cagcactcac aaataaagga acgccaatga aaattatact
    18721 ctgggctgta ttgattattt tcctgattgg gctactggtg gtgactggcg tatttaagat
    18781 gatattttaa aaatcagata tgacatataa gttgtcaggt caggaactag atattttgtg
    18841 tacttgattt gcaaacagct ccggtgttgg tggcgtcaaa atcgctgaag cgttccccga
    18901 aggccggggc agcccacatg gatgtgggct gagggcgcgt cttacaagga cgtttcctcg
    18961 cgcccgaccc ggtagccgaa ggggataagc tgaaggtacc gcgcagcggc gattttgttc
    19021 gccagagccc gggggtgcag ggggaggagg cgattgtcct ccccctgcgc gctccttgcg
    19081 ccagtggcaa tatgtggctt agctcatgaa aggagcgcaa caagatgatg aagctgaata
    19141 agcgattcat tgtttctgat aaactgcgat tccactatta tcgcagctta ccgatatcaa
    19201 ttgcctgaat agcccttgca atatcagggg aattattcaa cacacgaaca tgctgaaata
    19261 attctgttgc ttcatcgtat tctttacgca aataactcaa ccactgttta atccgcgcta
    19321 cgtgatataa cccggtatcg ccctgctttt ccagacgggt atatttttgc agcaaagcaa
    19381 ccacctccgg ccacggcatt cgcggttcgt tatattttac tacccggctc aggttgggaa
    19441 tattgagcgc cccgcgacca atcatcactg agtcgcagcc gctgatcgcc atgcattctt
    19501 gcgcgctctg ccagtcccag atttcgccgt tggcaatcac cggaatattc agccgctggc
    19561 gaatctcgcc aatcgcctgc cagtcaatat gctccgcgcg gtaaccctgc tcttttgtcc
    19621 gtccatgcac caccagctcc gtagcaccag cctgttgaac cgcatcggcg atttcaaatt
    19681 tcttctcacc gctgtcccag cccagacgca ctttcacgct gacgggcaaa tgcgccggta
    19741 cagcttcacg catcgctttt gcaccctggt agatgagttc aggatctttg agtaacgtcg
    19801 ccccaccacc gctaccgtta accgttttcg acgggcagcc gcaattgaga tccacgcccc
    19861 aggaacctaa ttccaccgca cgggcggcgt tctctgccag ccattgtggg aactgaccca
    19921 acaactgcac gcgcaccagc gtaccggatg gtgtccggct ggcgttttgt agctcagggc
    19981 aaatgcgatg aaagactttc accggcagca gttgatccac cacgcggacg aactcggtga
    20041 tgcacagatc gtagtcgtta acttcggtca gcaattcacg caccagagag tcaagcactc
    20101 cctccatcgg agccagtaac acacgcatat catcacccgc aaaaaaatga ggcgctatgt
    20161 tagcgcctcc tgtgtttgca gacaaactga cttactgatt aattcgggca agatttttac
    20221 cctcacccta accctctccc gaaggggcga ggggaccgcc cgtgcgcctg attgggtgaa
    20281 gaagtgctta gcgtggttgc aaacagttat cttctttcca gccgtacaac cactccatgc
    20341 cgcgataaaa ttctgcctgc gatttccctt tccacagcag attacgtacc tggcgttcta
    20401 cccctgcggc gtggtaaacc cgccccattt cacgggtcga ccagacgatg cgcgcggtgc
    20461 gcggaattcg taccgactca tacagcgcaa aggcttgcgc cgcatcaccc tcacactgcg
    20521 ccagcgcctt acccagcgtt actgcatctt ccagcgccat acaggctccc tgcgccatat
    20581 attgcgccac cggatgggcc gaatcgccca caagcgttat gcgcttcgtt ccccacttcg
    20641 ccaccggttc acggtcagcg gttgaccagc gtcgccatga agtcggttta tccaacatct
    20701 ggcgcggacg ggggtgaatc ccttcaaagt aagagagcac ttcctcttta ctaccatcct
    20761 tcacgcccca ctcttcctgc tggcggctgt ggaatgttac caccaggtta tactgcttgc
    20821 cgccgcgcaa agggtaatgc accaagtgac aatgcggccc cgcccacagt accggtgcgt
    20881 taatgcgcaa atcttccggc atatcggcac gatcgataac cgcacggtag acaacatgtc
    20941 cggtgacacg cggcgcatcg cccaataagc tctggcgcac cactgacttc acgccgtcac
    21001 agccaatcag aatatcgccg gtccagctat tgccgcgatc gtcaaacacg gtgacatcgt
    21061 cttccgtctc gcgaatatcc acgacgttgg tggaggtgtg atattccacg tccggatgcg
    21121 tcagtaccgc ttcccacacc gaggcgtgaa tatcaacccg gtggatcacc gcgtaagggc
    21181 cgccgaaatg gtcacgaaag gcctgcccgg tttcaatgca caccacttct tcggcgttca
    21241 ccgcatccat catggtaatg tgatcggtaa acaccgcgcg ctggcgggca acttcgccga
    21301 cgccgaggct gtccagcgcc gaaaacgcat ttggccccaa ctggatgccc gcgccaatct
    21361 caccgatctc atgcgccttt tccagcagca ccactttgat ccccaggcgg gccagtgaca
    21421 gcgcagttgc cgcaccgccg atcccgcctc ccacaattac tgcgcgtgtc actttcgtca
    21481 ttatagttct cctggtcagg ctgaaatttt atcttgttga ttttctggcg ctgcggcaat
    21541 gaacgctggc agctgtgtgc aggcgtcgta caccgctttg cagcgtggat acccgctcaa
    21601 atcgcagttc atgcgtagcg cgttcgccca ctgcgggaca aggcagcaat ctgccagcgt
    21661 cggcgtctcg cccacacaaa attgccccga ctggttctgg cgcaacagtt gttccaccgc
    21721 gcttaaccct tgctggatcc agtgcgcata ccaacgtttt ttttcttctt cactgacgtt
    21781 cagttcttcg gtcaggtagc gcagcacgcg caagttattc aatggatgaa tatcacaagc
    21841 gatggcatag acaatttcca gcaccttgtt gcgggccggc tcttcctgcg gcaccagcgg
    21901 aacttgcggg tagtgcctct ccagccagtc aataatcgcc agcgattgtc cgagtgattc
    21961 gccctcgtca gtcagcagcg tcggcaccag cccgaccgga ttcatccgcc gataggccag
    22021 ttcattttgc tgaccaatac ggatattcac cccttcggtc tggtaattaa tgcctttcag
    22081 cgccagcgca atgcgtaccc ggtacgacgc tgaactatta aaaaaactgt acagcttcat
    22141 catgcacctc agacaatctt caccgcgata ggcgttaagc cctcgacgtt gcctgtgatc
    22201 acatcacctt tcactaccgg gccaacccct tccggcgtac cggtgaagat cagatcgccc
    22261 ggttgcagct caaagaagcc tgacagatag cagatggttt cattcaccga ccagatgaga
    22321 tggcgaatat cgctgcgctg atgatcttcg ccgttaacct gtaaccagat tggcgcatta
    22381 tcaataccgc cgatatcgcg ggctttgtgc agcggcgcga tgggcgctga gagatcaaac
    22441 gctttaccga tttcccacgg gcggcccatc tggcgcattt ccatttgacg gtcacgccga
    22501 gtcatatcaa gacccgtggc ataaccccag acatattcat gggcgtgttc cagcgggata
    22561 tcgctgcctt ttttaccaat cgccaccacc agttcaattt cgtaatggta gttatcggtc
    22621 tgtgccggat acggcagcgc cagcgtttca ccagcggcaa ccggaacaac ggcatccgcc
    22681 ggtttgcaga agaagaatgg cggttcacga tccggatcaa agcccatttc gcgggcgtga
    22741 gcggcatagt tacgtccgac gcagtaaacg cggcgcacgg ggaattgttc atcactgcca
    22801 acaaccggta cggtaacggg cgcttgcggt tcgaatacgt attgagtcat ttttctctcc
    22861 taaattaata acgcgcttcg cggaacagcc ccagggcttc ttgtaccggt ctgtccgaaa
    22921 aactaaataa aacggaatct tgcgtggtct ggaacgacac gccatgccag gtcggcacca
    22981 cgaagatatc ttttgcacta aagctgaagg tttcattgcc gatgatgacc tgcccgctac
    23041 cttccaccac atggtagatg gtgctgtcag tggtacgcgc aacgcgtgag gcaaaccctt
    23101 tcggcagcag ttgcaggaaa gcgcccatcg acggcatcgg atagccgccg gtgaccgggt
    23161 tgacgtagcg cattttgtag ccatcccact cgtcggcatc gcccagtcgg gttaaatcgt
    23221 gcagcacttc gcggctgcgg tcataacgat agttaaagat cggcgaggag ttccccgtct
    23281 gatgccgcag cggcaacata ttggcagcgt aacgcggcag ataatctccc tctttacgcg
    23341 ttaccggttg ttgctcttcc ggataatctt cggcaaaacc gcagcccaga atattcacta
    23401 atggcagatc cagcccatcg agccagataa ccggttcgtc gccagggtta ccgtgatcgt
    23461 gccagcgcca ctgcggggtc aggataaaat cgccttcatt cattggcgtg cgttcgccat
    23521 caacggcggt aaatgcccct ttgccttcaa caataaaacg cagcgccgac tgattatgac
    23581 gatgactcgg tgccacttcg cccggcatga tcagttgcaa accggcatat aaagtcgcgg
    23641 taatggaaga ctgcccgcgc agcgccgggt tttccagcac cagcacgcgg cgcacggcct
    23701 ctttcgcacc aatcaggccg ccgctctcca gtagcagcgg gcggatctcc tgataattcc
    23761 agtacgctgg cgcacagtta gcgttgggcg ttttcggcac cagatggtgc agtgactccc
    23821 acagcggggt caggttttgc ccggaaatat gctgataaaa ttgctcgcgg ctattttgat
    23881 tgttatcagt cattgagatg ctccttattc gttcacaacg cctgcgcgag gcagtgagtc
    23941 agggacagag gtcgactggc gaaccacggt aatcagcagg gttaacatca ccgcactgat
    24001 cgccgcaggc acagcgataa tgaaaaacag ggtgtcgaaa gagaaattca tcgccatcat
    24061 cacgccgcca gacagcgaac caacaatcgc gccgcagcgg ccgatagcat ttgaccagct
    24121 cacgcccgtg gctcgacttt gtgttggata aagtgttgcg gtcagcgcat tcagccccac
    24181 ctgcgatccg ctaatgccaa tgccagtgcc gaaaatcgcc agcgccatca accataatcc
    24241 atcctggctt aagccaatca tcacgatgca tatagcgcct atggcatagc tcagcgtcag
    24301 cacgcggaaa ggattgaact tatccatcag cacacccagc gccagcgcgc ctaacgtacc
    24361 accaatctgg aacgcggcgg tgacccagga cgcgtgttgc aggtcgatgc cgcgatggtt
    24421 cagtagcgtc ggcatccagc ttgagagcag gtaaataatc agcaggctca taaagaacac
    24481 tacccacagc atcaacgtga tgggtaactg acgtcccata aacagttgac gaatgctgcc
    24541 ttttgtaacg gaggctgatt caatgagata aaagtgggta tcgacataac gctcgcgggt
    24601 gatggcgctg acggttttcg caatcaccgc ctgcggtagc tggcggcgca cctgccagcg
    24661 tggcgattcc ggcagaacta ccagcagtgc gacaaacagc atcagcggta acacgccccc
    24721 caacaccaga atgccgtgcc agccaatcac cggcaccagc tgcgcactga caatcccgcc
    24781 aaacgccgaa cccagcgtaa agccgcaaaa catcagcgtc accagcgcgc cgcggcggcg
    24841 ggcaggcaga tactccgagg tcatggtgat ggtgttcggc atcgcgccgc ccagacccag
    24901 gccggtgaga aagcgcagga agaccagcgt ttgcagatcc ggtgagaaag ccgaggcgag
    24961 gcttaacgcg ccaaacaaaa agacgcacag ctcaatcacc cgcttgcgac caaagcgatc
    25021 ggacaacggg ccacaaagca gtgcgcctgc ggttaatcct agcagacccg cgccaaaaag
    25081 cggggcgaga tcgcctgcgg tgagttgcca gtgagtgcga atatcagggg cgataaatcc
    25141 gatagccgcg gtgtcaaaac catcgagcat taccaccaga aaacagcaga tgataacgcg
    25201 ccactgcatc ttgccgaccg gagcagcatc gataagtgct tgtaaatcac gtcgttgagt
    25261 catagtaaat gcctcagtgc aggtaaggta gatgtttctt ttgttttgta tatgttgcga
    25321 tattgtgatt aaagagatga gatgtacaat ggcatttcgg gcagagagat aaccttgagg
    25381 ttatggcaaa tggcgaactg ggcgcaaaaa ttaaaactac atcaccttga aatgctggtg
    25441 gcgctgggtg aacagggcaa tctaacccat gtcgcacgaa tgatgaatat cacccaacct
    25501 gcgctttcga aatggttgtc acaactcgaa gatgagattg gaatcacact ttttgagcgg
    25561 cacagcaaag gattacgtcc gactgaaggc ggcaaattgt tattgcaaca tgcccaacgg
    25621 ttaatcaatg acatggagcg atcgcaacat gaactggccc gttttaaaca aggtggactg
    25681 gtcggcagtc tgaaaattgg ctgttctccc gtcgccactg actgcgtatc ccaggcgatt
    25741 ttgcatttgc ttacgaagat gccaactcta cacctgaata tcgaagagaa ggtgatgacc
    25801 ccattgctgc aagatctatt ggccggttct gtcgatgtgg tcgttgggcg aattggcggt
    25861 cgcgcattgc agctgccact gaattatcag gtgctgtaca ccgagccagt atgctttgtc
    25921 gcccgccctg agcacccact ggcgaaatac gccaccctca gctggaatga tcttgccaac
    25981 tggcgctgga tcgtctggcc gacgggtacc cccattcgca ttagtattga taatgcgctg
    26041 gtagaccacg gcgtaatgct gccagaaaac acgattgagt cggcgtcgat gaacgtcagt
    26101 accaacctgt tacaaagcag cgatatgatc tctattttgt cattacgcct ggcgcaacgt
    26161 tatgccagcc agggacaact ggtgatactc aacctgccaa agatagaaca gaaaggtagc
    26221 gtcggtgtgt tctggcgcaa taacgaaacg ccttcttatg ctttaagtca gtttctgcac
    26281 tttttacagc agaattagtg tgctgaacgg ggcgctcgcc cgaggttacc tccctaaaat
    26341 tcaactatgc tgagaattct taatgtgatc ggtagcacgt tttacgaatt aattgtatga
    26401 tgaatccatc tcatctgggg tgttgattat gagtaagaca ctgaacatta tctggcaata
    26461 tttacgcgct ttcgtcctga tttatgcctg cctgtatgca ggcattttca ttgcttccct
    26521 gctaccggta accattccgg gcagcatcat cgggatgctg atcctgtttg tcctgctggc
    26581 cttgcaaatt cttccggcga aatgggtcaa tccggggtgc tacgtactga ttcgctatat
    26641 ggcgctattg tttgtgccga ttggcgtagg cgtcatgcaa tattttgatt tgctccgcgc
    26701 acagtttggc ccggtagtgg tttcctgtgc aatcagtacg ctggtggttt ttctggtggt
    26761 gagctggagt tcgcaactgg tacacggtga acgtaaagtc gtaggtcaga aaggatcaga
    26821 agaatgatgg cgaatatctg gtggtcatta ccgctgacgt tgattgtctt ttttgccgcc
    26881 cgcaaactgg cggcacggta taagtttccc ctgcttaacc cgttgctggt agcaatggtg
    26941 gtgatcattc cttttttaat gctgaccggc atctcttacg acagttactt taaaggtagc
    27001 gaagtgctaa acgatctgct gcaaccggcg gtcgtcgcgc tggcctatcc tttatatgag
    27061 cagctacacc agatccgcgc gcgctggaaa tcgatcatca ccatctgttt tatcggcagc
    27121 gtggttgcga tggtgacggg gacttccgtg gcattgttga tgggcgcttc accggaaatc
    27181 gccgcgtcaa tcctgccgaa atcagtcacc acgcctattg caatggcggt tggcggcagt
    27241 attggcggta ttccggcaat cagcgcagtt tgcgtgattt tcgtcggcat cctcggcgcg
    27301 gtatttggcc atacattgct taatgcgatg cgtattcgta ccaaagctgc gcgcggtctg
    27361 gcaatgggga ctgcctcgca cgccctcggt acggcgcgct gcgccgagct ggattatcag
    27421 gaaggtgcat ttagttcgct agcgctggtg ttatgcggga taattacttc gctgatcgca
    27481 ccgatccttt tcccgattat tctggcagta atgggctaaa atttgcgatg cgtcgcgcat
    27541 ttttgatgta agtttcacgc gttgcataat taatgagatt cagatcacat ataaagccat
    27601 aacgggttcg taaactgtta tcccattaca tgattatgag gcaacgccat gcatccacgt
    27661 tttcaaaccg cttttgccca acttgcggat aacttgcaat ctgcactgga acctattctg
    27721 gcagacaagt acttccccgc tttgttgacc ggggagcaag tctcatcgct gaagagcgca
    27781 acggggctgg acgaagacgc gctggcattc gcactacttc cgctggcggc ggcctgtgcg
    27841 cgtacgccat tgtcgaattt taatgttggc gcaattgcgc gcggtgtgag cggaacctgg
    27901 tattttggtg ccaatatgga atttattggt gcgacaatgc agcaaaccgt tcatgccgaa
    27961 caaagcgcga tcagccacgc ctggttgagt ggtgaaaaag cgcttgcagc catcaccgtt
    28021 aactacacgc cttgtggtca ctgccgtcag tttatgaatg aactgaacag cggtctggat
    28081 ctgcgtattc atctgccggg ccgcgaggca cacgcgctgc gtgactatct ggcggatgcc
    28141 tttgggccga aagatctgga gattaaaacg ctgctgatgg acgagcagga tcacggctat
    28201 gcgctgactg gtgatgcgct ttctcaggcg gcgattgcgg cggcaaaccg ttcgcacatg
    28261 ccttacagta agtcgccaag cggtgtcgcg ctggaatgta aagacggtcg tattttcagc
    28321 ggcagctacg ctgaaaacgc cgcattcaac ccgactctgc caccgttgca gggagcgtta
    28381 attctattga atctcaaggg ttatgattac ccggatatcc agcgcgcagt tctggcagag
    28441 aaagccgatg cgccgttgat tcagtgggat gccacctccg caacactgaa agctctcggc
    28501 tgtcacaata tcgaccgcgt gcttctcgct taagcctggt gccggatgcg gcgtgaacgc
    28561 cttgtccggc ttgccagccc tctcctggtg tcgaaaatcc cggcaaacag tttgtcgttt
    28621 cttgcgcaaa accagcgggt aaagtagcct gatggaaatt ttccttagat cgagtctcct
    28681 gcatgttaaa gcgcgtgttc ctcagcctgt tagtcctgat cggcttgctg ctgttgactg
    28741 tgctcggcct cgatcgctgg atgagctgga aaaccgcgcc ttatatctac gacgaattgc
    28801 aggatctccc ctaccgccag gtcggtgtgg tgctcggaac agcaaaatat tatcgtactg
    28861 gcgtaattaa tcagtattat cgctaccgca ttcaaggagc gattaatgcc tataacagcg
    28921 gtaaggtaaa ttatctatta ctgagcggcg ataacgcatt gcaaagttat aatgagccga
    28981 tgaccatgcg caaagattta atcgctgctg gtgtcgaccc atcagatatt gttctcgatt
    29041 acgcaggctt tcgtacgctg gattccatcg tgcgtacacg caaagttttc gatactaatg
    29101 atttcattat tatcacccaa cgtttccact gtgagcgggc attatttatt gcgctgcata
    29161 tggggattca ggctcagtgt tatgccgtac cgtcaccgaa agatatgctg tcagtacgta
    29221 ttcgtgaatt tgccgcccgt ttcggtgcgc tggctgacct ttatattttt aaacgtgaac
    29281 cgcgtttttt agggccgctg gtccctattc cggctatgca ccaggtgccg gaagatgcgc
    29341 aggggtatcc cgccgtcaca cccgaacagt tacttgaatt acaaaagaaa caaggaaagt
    29401 agttatggat gtacagcagt tttttgtcgt tgccgttttt ttccttatcc cgatattttg
    29461 tttccgcgaa gcatggaaag gctggcgcgc aggcgcgatt gataaacggg ttaaaaatgc
    29521 accggaaccg gtgtatgtct ggcgagcaaa aaatcccgga ctctttttcg cttatatggt
    29581 ggcatatatc ggcttcggaa ttttatctat cggcatgatt gtttatctta ttttctatcg
    29641 ttaatatttt ctcttttctg ttaattattg agaataatta ttacttcact caataagctg
    29701 cggatataac attctgaacc gcagctattt gtgaagcctt tcacagttta aatttcccca
    29761 cgcgcatagc cttaatatca gcacgcaata tttatttagc gttcgcctta attactcata
    29821 gcattaagga agatcacatg ccgcaacaaa attatctgga tgaactcact ccggctttta
    29881 cgccgttact ggcgattaaa gaagcctctc gctgtttatt atgtcacgac gctccctgta
    29941 gtcaggcttg cccggcacag accgatccgg ggaaatttat tcgctcaatc tactttcgta
    30001 attttaaagg cgctgccgag acaattcgcg aaaataatgc cctcggtgcc gtttgtgcca
    30061 gagtgtgccc gacggagaaa ttatgccaaa gcggttgtac ccgtgccggt gttgatgctc
    30121 ccattgatat cggccgctta cagcgttttg ttactgattt tgaacaacaa accgggatgg
    30181 aaatttatca gcccggtact aaaacgctcg gcaaagtcgc gattattggc gcaggtcctg
    30241 ccggattgca ggccagtgtg acgctaacaa accagggtta tgacgtcacg atttatgaga
    30301 aagaagcgca ccccggtggc tggttgcgta acggtattcc gcaatttcgg ttaccgcaat
    30361 cagtgctgga tgcagaaatc gcccgtattg aaaaaatggg cgtgaccatt aagtgcaata
    30421 acgaaatcgg taaaacactc accctggagc aactgaaagc ggaaaaccgc gcggtactgg
    30481 tcaccgtggg gttatcaagc ggttccgggc taccgctgtt tgagcatagc gacgttgaga
    30541 ttgccgtcga cttcctgcta cgtgcacgac aggcgcaagg cgatatcagc attccacaaa
    30601 gcgcattaat tatcggcggc ggtgatgtcg cgatggacgt agccagcacg ctgaaagttc
    30661 tcggctgtca ggcggtaact tgcgtagcgc gtgaagagtt agatgagttt ccggcaagcg
    30721 aaaaagagtt tgccagcgcc cgggagctgg gcgtttcgat catcgatgga ttcacgccag
    30781 tagccgtgga aggcaataaa gtcacgttta agcatgtacg gttatcgggc gaactgacga
    30841 tggcggcaga taaaattatt ctcgccgtcg gtcagcatgc cagactggat gactttgcga
    30901 agttagagcc gcagcgtaac accatcaaaa cacaaaatta tcagacccgc gatccgcaag
    30961 tctttgctgc tggcgatatt gttgagggtg acaaaaccgt ggtctatgcc gtgaaaaccg
    31021 ggaaagaagc cgccgaggcg attcatcact atttagaggg agcttgctca tgttaacgaa
    31081 agatctttcg attacttttt gcggcgtgaa gtttcccaac ccgttctgcc tctcttcttc
    31141 gccggtaggc aactgctatg agatgtgtgc caaagcctac gacacaggtt ggggcggcgt
    31201 ggtgtttaaa acgatcggct tttttatcgc caacgaagtc tcgccgcgtt ttgatcatct
    31261 ggtgaaagaa gataccggtt ttatcggctt caaaaatatg gaacagattg ctgaacatcc
    31321 gttggaagag aatctggccg ccctgcgtcg gctgaaggaa gattacccgg acaaagtatt
    31381 gatcgcttcg atcatggggg aaaatgagca gcaatgggag gagctggcgc gcctggtgca
    31441 agaagctggc gcggatatga tcgagtgtaa cttctcctgt ccgcaaatga cttctcatgc
    31501 gatgggtagc gatgtcgggc aaagcccgga gctggtagaa aaatattgtc gggcagtgaa
    31561 acggggttcc acgctgccaa tgctggcgaa gatgacgccg aatatcggtg atatgtgcga
    31621 agtggcgctg gcggcgaagc gcggcggcgc agatggcatt gcggcgatta acaccgttaa
    31681 atccatcacc aatatcgatc ttaatcagaa aatcggtatg ccgatcgtta acggaaaatc
    31741 gagtatttcc ggatattccg gtaaagcggt aaaaccgatc gccctgcgct tcattcagca
    31801 aatgcgcacc catccagaac tgcgcgattt cccaatcagc ggtatcggcg gcattgaaac
    31861 ctgggaggat gcggcggagt ttttattgct cggcgcagca acgttacagg tcaccaccgg
    31921 cattatgcag tacggctatc gcatcgtaga agatatggcg agcgggttat cgcattatct
    31981 cgccgatcag ggatttgatt cgttgcagga gatggtaggt ctggcgaata acaatattgt
    32041 cccggcggaa gatttagacc gcagttatat tgtctatccc cgtatcaatc ttgataaatg
    32101 tgttggctgt ggacgctgtt atatttcctg ttacgacggc ggtcaccagg cgatggaatg
    32161 gagcgagaaa acccgcacac cgcattgtaa taccgagaaa tgtgtgggtt gtctgctttg
    32221 tggtcacgtc tgcccggtgg gttgtattga gctcggggaa gtgaagttta agaaaggcga
    32281 gaaagaacac ccggtaacgt tgtaaaacca gagacgcatc cggcatttgg ttcctgtcgc
    32341 cggatgcggc ttgaacgcct tatccggcct acaaaaccat acaaatcagt atgttgagct
    32401 cattgcaggc cggataaaac gcgccagcgt tgcatccggc atttggctcc tgtcgccgga
    32461 tgcggcgtga acgccttatc cggcctacaa aaccacacag atcatgatgt tatgtgcgtt
    32521 tatgaccgaa tgcggaccac aatcatttca cttcttacgc gcgtacttca gtgaatccag
    32581 cgctacggcg aagataataa tcgccccttt gatgatgtac tgccagtatg ggtttacgcc
    32641 gatgtacgtc aggccatagt tgatgacggt aaaaataatt accccggtca ccacgccaat
    32701 caccgtcccc acaccgccgc tgaacgatac accgcctacc acgcacgccg cgatagcatc
    32761 cagttcatac ataaagccga ggttgttggt ggcagagccg atacgtccgg cttctaacat
    32821 cccgccaaag gcatagaaca cgccagacaa cgcgtagatc atcagcaggt tcaggccgac
    32881 gttgacacca gatacttttg ccgcttccgg gttaccgcca atggcaaaaa tgttcttacc
    32941 gaagcgggtt ttgttccaca acacccagac gaacgccacc gcaatcaacg cgtagaaggt
    33001 gatgtaagag agacggaaac tccccagcgc gacaaagccc tgagcaaagg tagagaagcc
    33061 actgtcaaaa ccagaaattg gcgacgcccc gacaaagtcg taataaagcg agttgatgcc
    33121 atagacgatg atcatcgtgc cgagcgtggt aatgaacggc gtcacgttga gataagcgat
    33181 aatcagaccg ttgatcaaac cgatcaccgc accaatggca cagacaatca gaataaccag
    33241 cgcaatcggc atcgtcgcca tttccgggaa cactttgttg gcgttatcca tggactgcaa
    33301 taatgtcgct gccaccactg ccgccagccc tacttgacgc cccgcagaaa ggtcggtccc
    33361 ctgggtgaca attaaccctg ccacaccgag cgcgataata atacgcaccg atgactgggt
    33421 gagaatatta cttaagttca acagacttaa aaatgttggg gcctggaaaa taataatcgc
    33481 aagcaaaact aaaagaacga cgtaaatacc gccctctttc aggtaagtaa gaaaactttt
    33541 cttatttaac gcactcatgg gaagccccta atcttaaagg tgcaaagacg caagacgtag
    33601 aatttcgttt tgcgttgttg tttttgtatc gacaattccg gaaacgaggc cattgctcat
    33661 aaccagaata cggtctgtta tccctaacaa ctctggcatt tcagaggaga taataataat
    33721 ccccttgcct ttcttcgcca gttcggcaat taactgataa atctcaaact tcgccccaac
    33781 atcaataccg cgcgtcggtt catcgagcat taatatttct ggttgcgtta acagccagcg
    33841 cccaataatc accttttgct gattaccacc tgagagcgaa ccaatttgcg tccgatgccc
    33901 cggtgttttt acccgcatcg aatcaatcac ccactgggta tcgcttttca tccgcgagtt
    33961 atccagtaaa ccaactttat ttttgtagtt gcgaatattg gaaattaacg agttaaaacc
    34021 aatatccaga taggcataaa ttcccgttga gcggcgctcc tcagttacca gtgcaaatcc
    34081 gtggtttatg gcttcgttgg cattatggtt attgatcttt ttgccgtgca acgtaatggt
    34141 gccagccgat ttctcgcgaa taccaaataa cgtctcaaca atatcggtac gtttcgcccc
    34201 caccagaccg gcaataccga ggatctcccc tttatgcaga tcaaacgaga catcgcgaat
    34261 cgacggctgg cgcagtgacg tcaggttacg tacctcgagg atgacttcgc ccggcttgtt
    34321 ttctttgtca ggaaaacgct ggttaagaga acgcccaacc atcatggcga taatcttgtc
    34381 catcgtcagc cctgccagcg gttcggtggc gatccactga ccgtcgcgca atacggtaac
    34441 ttcatcacat aactggaaga tttcctccat tttatgcgag atataaacaa taccgcagcc
    34501 gcgctctttt aatttacgaa taatagtgaa cagatgattg acctcttttt cggttaacga
    34561 agaagtcggt tcatccataa tcacaatttt cgcgttatag gaaaacgctt tggcgatttc
    34621 gatcatctgc atttgcgaaa cagataatgt gccgacgcgc gcacgcggat cgatatcaat
    34681 atccagttca tcaaaaatcg ctttggtttc gcggtacatt ttgtcctgat cgacaaacat
    34741 gcctttggtg gggtatcgcc caagccacat gttgtccatc accgaacgtt gtaataccag
    34801 gtttaactcc tggtgtacca tcgaaatacc attttccagt gcttctttgg ccgaatggaa
    34861 atcgatctct ttaccctgga ataaaatggt gccggagtct tttttataaa taccaaacag
    34921 gcattttaat aatgttgatt ttcctgcgcc gttttccccc attaatgcat ggatagaatg
    34981 tggccggact tttaaattaa cgttatcaag tgccttaaca ccaggaaaag acttgttgat
    35041 accgctcatt tccaacaagt attccccgga agacggagtc gttgagctga ccatataatt
    35101 ttaccttgtt ggccatacaa taagggcgca gtaatagact gcgcccaatc agtcttattt
    35161 cttgctgaat tcagccaggt tgtctttatc tacgccaaca taaggtacgc ggaccacttt
    35221 gttgtcgatt ttccagttgg tgccatcagc cgcaccttta ccatcggcca ggtttttcgc
    35281 cagatcaaag gtcgctttcg cctggttgtt agcatcgttc agtacggtgc ccgccagtgc
    35341 accggatttc accagcgcca gcgcttctgg cagagcatcg acgccaaaca ccggaatgct
    35401 ggacttgttg tgtgctttca gtgcttctac cgcgcccatt gccatcgcat cgttgttggc
    35461 gataaccact tcgattttgt tggcgttcgg gccagacagc caggcgtcca tcttatcttt
    35521 cgcctgagcg gtatcccaca tagcggtatc taactgtaac tgttcagttt taatgccttt
    35581 gtcgttcagc tctttaatca cgtaagtggt acgtgcttct gcatccggat ggcccggttc
    35641 acctttcagc agtacgaatt gaatctgacc gtctttattc agatcccaac cctgattcgc
    35701 cgcccagtgt ttagcaatca aatcgccttg aataatgccg gactctttgg agtcagtgcc
    35761 aacgtagtag gctttgtcgt agctatccag cgccttacga gacggttctt tgttaaagaa
    35821 taccaccggc acattttgcc cacgcgcttt ctcaatcacc gtacccgcag ctgccgggtc
    35881 aaccaggttg attgccagtg ccttcacccc tttcgccagc aatacgtcga tctgatcgtt
    35941 ctgcttggac tggtcattct gtgaatcatt catcagcagc tgaacatctg gcgcggcttt
    36001 cgcatcttgc tcaatagcct tgcgcactac agacataaag ttatcgtcgt acttatagat
    36061 tgttacacca atgcgagtat cagcagcgtg tgcagtggca ccgaataaca tgctggccat
    36121 cacagcagac agggttaaca ccttcttatt catggtatct ccggtttttc ttatgcaggg
    36181 tagtgcttga gataaatgct cggcggggca gtagagttaa tgaagtgtta ctgaacgccg
    36241 aagctcactt tttaaaattc gttcttccat gcccggtaac gctccagaaa acggctttaa
    36301 ttgttgttat gacgcattac ctcggtgaaa gtgattattc actgttacat acgggttaca
    36361 acgttaaaac ggtgcaatca tagctatcac attgttaaga tactgtgaaa tcactcacag
    36421 attgaaagcg gttacatcgc ctgatttgtt gagttagtga tcgccgccgc attctggcgt
    36481 gttgcgacag aatggcgacg cactaacgtc ggcataaaac agtggctggc acgaggatca
    36541 atattgcctg ctgccccctg caaggccagt tcggtggcta atttcgccat tgaagcaatg
    36601 ggataacgca cggtcgttaa ttgcgggtcg gtgtaacggg cgatgggaat atcatcgaaa
    36661 ccgatgattg agagatgtaa cggaatcgca atgccattat cttttaatgc tgtcagtgcg
    36721 ccagcagcca tattgtcgtt ataagcaaat acagcggtaa gttgtagatt gcgccccagc
    36781 agttcaacca tcgccgcctc accgcccggc atgtccggcg taccagtgcc aatccagctt
    36841 tccggcggaa taatatcctg ctctttcaac gcactcatcc agcctgcttt acgcatggcg
    36901 tcatcttcaa tgccgtggct ggaagaaaga taaccaatac gttgatgacc gttattcagc
    36961 aacatgcgcg tcgccattcg ggcaccgctg agattatcca ggcaaacgca acgatgggtg
    37021 taccccggca caacgcggtt gattaacacc ataccgggaa tgttatccat aaattgcgcc
    37081 agttcatcgt cactcaatgc ttttgagtga acaatcaacg cattacaacg ctggcgaatt
    37141 aacacctcaa tggcgtgacg ctctttttcc gcttcatgat agctattgcc gattagcacg
    37201 tatttctgat gctgctgagc gaccagatcc accgctttta ccagcgcgcc gaaaaacgca
    37261 tcagaaacgt ccatcaccac cacgccaatg gtgtcgctaa cctgagttgc cagcgcctgg
    37321 gcattggcgt ttggccgata atccagctca ctcacggctt tcattactgc ttcacgcgtg
    37381 tcggcactga ccagcgtgct gttattgagc acccgggaaa ccgttgccac agagacgcca
    37441 gcctgacgcg ctacatcacg aatggtgatc atattcacta cctgttatga gattgcagta
    37501 aatgactgct tgctgccggc tattttgtca gcactgaata caggacttcg tgaatcgagt
    37561 cacagcaatg gaaacggtta cagccgtttt gttaacgaat gtgatccaga tcgttatctt
    37621 gatgttttag atattgccgg tccggcagca cgcaaagtta actgacgcca gagccattcc
    37681 accggcccct ggcggaagta acgcagccag ataacagaga agagaatatt cgccagccat
    37741 accggaataa caaacgccag caattccaga cggtcaaact gcataaacag accgaggtgg
    37801 taaaaaagcg tggtacaaat cagcgtttgc aatagatagt tggtcagcgc catccgtccg
    37861 acgcaggcga tcgccagcac cagcttaaag cggctcaatt gcggccagaa gccataaaac
    37921 agcgacgcat agccaatcgc ctgaaacggc gcactcagtt cccgcggcat ttgaagtaag
    37981 aaggcacacc agcgatacgc ccagtccagc tgccattgca gggcgatggc aggaaggtta
    38041 atgatcaccc caatcgccac tagcacaaaa ccagtacgac gatagtgacg taagctgaac
    38101 tgccctttca gccagccact gcgcatcaat gcggcaccaa tgagcatcat cccagccagt
    38161 tgccagccat actgtgcgcc cagtgccaat aaactgttcc caacaccatc ggcacgatta
    38221 ctgatcgctt caacgccgcc gtgaagcttc cagtattttt catataaaat agccgatgca
    38281 tccggcgtcc aggcgcggct agtctggcta tcggaaatca accccaataa cagcaaaacg
    38341 ccaagcccca ccagataaag catgacgccg gtattaaaaa ggctttttac cgatggcgca
    38401 tcgcgcacca gccgccagca gattaagccc accagcccgt aagccagcag aatatcgccg
    38461 tcccagaaca ataaaccgtg aataaagccc agcaagacta acagcgttaa ccgcgactgg
    38521 atccagcgtc tgccacgggg cagcaacatt tgcaggccag caccaaacag cagcgcaaaa
    38581 agcgtgagga atttcacctg gccgatgaga tcgagaaatg cccaggtcca tgcatcctgc
    38641 ggcgtaatag cgccgtacca ggcgggatta agataagccg cctttggtag cccaaaggcg
    38701 ctgatgttta atagcaggat ccccagaatg gcaacgccgc gaacaaagtc gagcgtgacg
    38761 ttgcgctcca tggttcctgc ctttaaatca gttgtgatga cgcacagcgc gcagaaactc
    38821 gtggcgcgta ttctgactgg atttgaacaa tccaccaaga gaggtcgttg tcgtggcact
    38881 ggttgcatcg cggatgccac gcgccttcac gcaataatgc accgcgtcga tagagacagc
    38941 cacgttattg gtgcccagca gcgtttgtag cgcaatgaga atttgctgcg tcagacgttc
    39001 ctgcacctgc ggacgctggg caaagaactg cacaatacgg ttaatttttg acagaccgat
    39061 caccgaatct ttcgggatat aagccaccgt cgctttgcca tcgatggtaa caaaatggtg
    39121 ttcacaggta ctggtcagag tgatatcgcg cacggtgacc atttcatcga ccttcatttt
    39181 gttttcaatg agggtgattt tcgggaagtt ggcgtaatcc agaccggaga aaatttcatc
    39241 gacatacatt ttagcgatgc gatgcggcgt ttccatcaaa ctgtcatcag ccaggtcgag
    39301 attcagcagc tgcatgattt cggtcatatg accagcaata aggcttttgc gcgtttcgtt
    39361 atccatttca tgcacgggcg ggcgcagcgg tgtttccagt cctcgcgcaa ctaacgcttc
    39421 atgaaccagg gccgcttctt tactgagtga tggcatttat gatttctcct gcaggtgtga
    39481 cgcctccgcc ctgcgtgggg gcaaagttat taagctgatt tacagcctga ttattgtgcg
    39541 tgaggcggcg cacataatcc agtattcaca gcgataatta ttgtaattgc cgctgccttt
    39601 catcagcaga tgttaaaaca tcgttatgca aatacggaag tgaaagttac tcacagcaca
    39661 ttgaataaac ggtatgatga agaaattgca aacaacacaa caaggagcca cgcatggaaa
    39721 tgctcgaaga gcaccgctgt tttgaaggct ggcagcaacg ctggcgacac gactccagta
    39781 ccttaaactg cccgatgacg ttcagtatct ttctcccacc ccctcgtgat cacaccccgc
    39841 caccagtgct gtactggctt tccggattaa cctgcaatga cgagaacttc accaccaagg
    39901 cgggtgccca gcgcgtggcg gcggaactgg ggattgtact ggttatgcca gacaccagcc
    39961 cgcgcggcga aaaggttgcc aacgacgatg gctacgattt aggccagggc gcaggctttt
    40021 atcttaatgc cacgcaaccg ccgtgggcga cgcattaccg gatgtatgat tatctgcgtg
    40081 atgaattacc ggcgctggtt cagtcgcaat ttaatgtcag cgaccgctgc gctattagcg
    40141 gtcattcaat gggtggtcac ggtgcgctga ttatggcgct gaaaaacccg ggcaaataca
    40201 ccagcgtttc ggcctttgcg ccaattgtga atccgtgcag cgtcccgtgg ggaatcaaag
    40261 cgtttagccg ctatttaggt gaggacaaaa atgcatggct ggaatgggac agttgcgcgc
    40321 tgatgtatgc cagtaacgcg caggatgcga tcccgacgct tatcgatcag ggtgataacg
    40381 atcagtttct tgctgaccaa ctgcaacctg cagtacttgc agaagccgcg cgccagaaag
    40441 catggccgat gacgctgcgt attcagccgg gatatgatca cagttactac ttcatcgcct
    40501 cttttataga ggatcacctg cgcttccatg cgcagtattt actgaagtga aagtccgccc
    40561 ggttcgccgg gcatcttctc atcagaagcg ataatccact gccataaagt aacgacgtcc
    40621 gtcttcgtta tagctgtagt cgtcacgact gagatccttg tcgccaaggt tcagcacgcc
    40681 tgcgcgcagt ttgacgtctt tagtcacctg ccaggccgcg ccggtattcc agatggtata
    40741 accgcccggt gttttagccg tcgcgctgtc ggctcgtttc tgcccggtat agtgaccaga
    40801 aacatagaat gaccagtctt ccagcgccag cggtttccag tccagcgtac cgttagcggt
    40861 atggaacggc agatcggata gcggtttgtt ttcgccgttg ctgacatcac gaccatcgtt
    40921 gtaggtgtag ttgagtgaca gtttccattc atcgttgaac ggaattttca gctcggtttc
    40981 cacgccctga atacgtgctt tgttaacgtt gtagtagcta aataccggca caccgttcgc
    41041 tgtaaaacca acaaagtttt ggtagcccgg tgcagcattg acgtcagacg tacggctgat
    41101 gctgatacga tctttcacat cgttacggaa aacggtaacg ctggattcaa ccccttccag
    41161 ccagccttct tcacccatgt agtaaagccc cagctcccaa ctttcgctgg tttctggttt
    41221 cagatccggg ctacccacaa tcttacatgc gccacggcag gaattgctcg tccagtcagg
    41281 gctaagttgc aacagagaag gtgctttaaa tgccgtcgcc cagccccctt tcaccgttac
    41341 ggtgtcggtg gcgttataaa ccaggtaggc acgcggactc cagtgttcac cgtaggtttc
    41401 gtgatcgtcc atacgcacgc cggtcgtcag cgccagcggc tcgaagatcc gccattcatc
    41461 ttccacaaac agcgcgtact ggctggcaga cgttttggag ctggttcccc cggtcaggtt
    41521 caccgcatcg ctaagtttgt cgtgacgcca ttcaccgcca accgtgagaa actgattaat
    41581 cgccgtcagc ggcaacgtgt atttgccgtc gaccgtattg ctttcggaag ttatcgggct
    41641 gctgttgcca gggtttttgt tctcgacttt ctcaccgtag tatttcagtt cgctggtgcc
    41701 gtaatcccaa cgcccattat ggctgacgga gtagttctgg cgttccaggc ggtttttgtc
    41761 cagcgagtcg gaatcacgat cctgacggtc gaaaccgtat ccggcagtaa aatcgtgatt
    41821 ttgattcggt gtccaggcaa attcgacatt gccgtcgcgg ctggagaatc cttcaatacg
    41881 cggcgtttct ccggtatcgg tggtcgttga gttttgcggg tcatcctttt cacgttttgc
    41941 caggctgccg taagctttca ttcccagcac accatcaatt aatggtccac tggtaaagaa
    42001 ctgaccgtta taggtatcac cgcgatcgcg atgttcctga atggtggtat cgacggtaac
    42061 ggtacccgac catttctgac cgattttttt ggtgatgata ttcactacac cgccgagcgc
    42121 atcggaaccg tacagcgacg acatcgggcc acggaccact tcaatacgtt cgatggaatc
    42181 gaccgggatc cagttcagat cgaaatcatt gtggcggaag acggcattgc gggagttaac
    42241 gcgtttaccg tcgacaagaa tcagggtgta gctgctgtcc agaccacgaa tactaacgcc
    42301 cttacggtta tccccttcgt tcgtcagttg tacgccaggc acttctttga ggacatcctt
    42361 cagattctgt accggttttc gctgcaggtc ttcctgggta atgacgctga tactggcggg
    42421 tgcatcttta aggttttgtt caacggaaga tgcagtgaca accatcgttt cgccatcgtc
    42481 atcgaccgct aacacaggcc atgcacaaga aatagcggac aaacacagcc cgacccgtac
    42541 gaaagggttc aacctaaaca ttccatatct ccatgaggta actacgaaaa taaaatgggt
    42601 tatcgctcac atcttcttca cgtccccttg cgtacggcag catcgcggtg gacttatttg
    42661 tatgcaggtg atcatcccag aaagccggac cagcctctga tttgtcagcc ttttttgatt
    42721 gcggcgtaac gataatgcaa acgataacaa ttatcaatcc aaatgttaaa ttttatatcc
    42781 gcatgatttg tcagggaaat ggaattgaaa aagccctctc ggttgagagg gcttagcaag
    42841 gaagggagaa acttatttct tatcgttctg cgggaacttc atttcgctgt agcgtacgaa
    42901 gtgggttcct ttaatcagct tgtagccgaa ccaaataatc aggaacagcg ggataccaat
    42961 atacgttgcc gctacgccgc cccagtcaat ggtatctttc aggaacgctt cgtagttctg
    43021 gcccaaagtg ataatcagac acagaatgaa tgcgaagatc ggccccaatg ggaagaaacc
    43081 tgaacggtac ggcagatcgt taatgtcgtg tccctgcaat acgtaaccgc gacggaagcg
    43141 atagtggcta atggcaatcc ccagccaggc gataaaaccg gtcatcccgg aggtattcag
    43201 cagccacagg tataccgtct ggttgccaaa catggaggtc aggaagcaca gaccggcaat
    43261 caccgtcgtc gcatacagcg cattacgcgg cacgccacca cgcgacagtt tagcgaaaat
    43321 gcgcggcgct ttaccgtcac acgccagggt gtacagcata cgagtagacg catacatacc
    43381 ggagttaccc gctgacagca ccgccgtcag aataactgcg ttcatcaccg ccgcagcaga
    43441 gagcagaccc gcgtgctgga acaccagggt gaacggacta acgctgatgt ctttaacatc
    43501 gttacgcagc aggctcggat cggtgtacgg aataatcagg ctgataatca ggatcgcgaa
    43561 cacatagaac aacaggattc gccagaacac ctgacgtacc gcgcgtggaa tgtttttccc
    43621 cggatcttcg gactcgcctg cagcaatacc gatcagctcg gttccctgga aagagaagcc
    43681 gacaatcata gctacgccga tcatcgccgc aaaaccacca gcaaacggcg cttcgccgat
    43741 tgtccagttg ctccagcccg caggctgcgc gcctttgaag ataccgataa tcatcagcac
    43801 gccaacgatg ataaagacaa taactgtcgt gactttgatc agtgagaacc agtattccgc
    43861 ttcaccaaag ccgcgaacag agatgtagtt cagcaggaag ataacgccga ggaacaacgc
    43921 actccagatc cagcccggtg tatccgggaa ccaccagctc atgaccagct gagctgcaac
    43981 caggtcaacg gcaatagtca ccgcccagtt gtaccagtag ttccagccca gcgcgaagcc
    44041 aaagccctct tcaacatagt tctgaccgta agtggcaaac gaaccggaaa ccggcatata
    44101 tgcagccagt tcaccgagac tggtcatcag gaagtaaacc atcaggccaa tcaacatata
    44161 cgagagcaat gccccgcccg gacctgcctg agaaatcgtt gcgccagagg caacaaaaag
    44221 acctgtaccg atggaaccgc caatggcaat catcgtcagg tgacgcgcct ttaattcacg
    44281 gcgtaagccc ggcgcttctg tggttttagt ttcggaaacc atacaaaaat gctatccatc
    44341 taaaaaatga ggcgcgattg tagcagacga ttggcaatcc ttccggcaga aatacacggt
    44401 tataagacac cttcatgatc gcccagggat tataagtaaa gcagccaatc tcttttctgg
    44461 cgaatgcatg ctgaaggcgc tgcgtcacga aatcgacaca gcaccagcat gttcttgtac
    44521 agcaacttaa cgcggcacat ttgcgggatc gcaatagtcc agaaagcgcc gtagcgcgtt
    44581 ggaaaggtgt ttttgccgat gatgtatacg ccacaacgta cgcatcaggc gcggcagagg
    44641 gaccgcaact tcacttaatg tgcctgcctg caattgatct tcaatcacac gtcgcgacag
    44701 gcaactaatt cccaacccat gacgcaccgc atgtttgatt gcctcggagt tacctaattc
    44761 catcgccatc tcaaacttcg gtaaatgtga cagcaacaga taatcgacaa tctcccgcgt
    44821 gccggaaccg cgttcacgca ggatccacgg cgcagcggcc agctgttcta aggtgaccgg
    44881 accacgggcc aacggcgaag tcggcgcggc gaaaaccacc agctcgtctt ccagccacgg
    44941 ttcagaaatg atttcagtgc tgtggcacgg tccttcaata aagccaatat caacgcggaa
    45001 atccagcacc gcttgcatca cgtcctggct attcccaacg ctaagttcaa tcggcaactg
    45061 cggataatca tggcgataac gggcgataac tgcaggcaga atgtagttac cgatggtact
    45121 actggcatag atacgaatcg cgccgttgtc ttcgcgaaac agttgttcga tttcaaccgc
    45181 ctgttcaagc aatgccaacg cacgcggata gagcagccgc ccgtgttcat taacaaccag
    45241 tcttttcccc acgcgatcaa acagttgcac gccaagctgc ccttccaggt cggtcaaggc
    45301 tgcgctcact gctgattgcg acaacgccag catcaccgac gcttgggtgg ttgatccact
    45361 tttcaatact tctgcaaaaa cttccaactg ccggagggtg atgtgcatag tcgctt
//
LOCUS       DAZKIF010000020        45018 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZKIF010000020 DAZKIF010000000
VERSION     DAZKIF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.020"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1063)
                     /gene="espY3"
                     /locus_tag="ACHOTW_001166"
     CDS             complement(<1..1063)
                     /gene="espY3"
                     /locus_tag="ACHOTW_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFP9843319.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKDGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNA
                     NCTNANFNNCRFKKCDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKS
                     DGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKTEAKSTEEKDI"
     gene            complement(1294..2268)
                     /gene="yajO"
                     /locus_tag="ACHOTW_001167"
     CDS             complement(1294..2268)
                     /gene="yajO"
                     /locus_tag="ACHOTW_001167"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP9843320.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(2323..4185)
                     /gene="dxs"
                     /locus_tag="ACHOTW_001168"
     CDS             complement(2323..4185)
                     /gene="dxs"
                     /locus_tag="ACHOTW_001168"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9843321.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(4210..5109)
                     /gene="ispA"
                     /locus_tag="ACHOTW_001169"
     CDS             complement(4210..5109)
                     /gene="ispA"
                     /locus_tag="ACHOTW_001169"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP9843322.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            complement(5109..5351)
                     /gene="xseB"
                     /locus_tag="ACHOTW_001170"
     CDS             complement(5109..5351)
                     /gene="xseB"
                     /locus_tag="ACHOTW_001170"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9843323.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5557..7005
                     /gene="thiI"
                     /locus_tag="ACHOTW_001171"
     CDS             5557..7005
                     /gene="thiI"
                     /locus_tag="ACHOTW_001171"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9843324.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            complement(7059..7649)
                     /gene="yajL"
                     /locus_tag="ACHOTW_001172"
     CDS             complement(7059..7649)
                     /gene="yajL"
                     /locus_tag="ACHOTW_001172"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP9843325.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(7612..8523)
                     /gene="panE"
                     /locus_tag="ACHOTW_001173"
     CDS             complement(7612..8523)
                     /gene="panE"
                     /locus_tag="ACHOTW_001173"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9843326.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8691..9182
                     /gene="yajQ"
                     /locus_tag="ACHOTW_001174"
     CDS             8691..9182
                     /gene="yajQ"
                     /locus_tag="ACHOTW_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP9843327.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(9310..10674)
                     /gene="yajR"
                     /locus_tag="ACHOTW_001175"
     CDS             complement(9310..10674)
                     /gene="yajR"
                     /locus_tag="ACHOTW_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9843328.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(10823..11713)
                     /gene="cyoE"
                     /locus_tag="ACHOTW_001176"
     CDS             complement(10823..11713)
                     /gene="cyoE"
                     /locus_tag="ACHOTW_001176"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP9843329.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11725..12054)
                     /gene="cyoD"
                     /locus_tag="ACHOTW_001177"
     CDS             complement(11725..12054)
                     /gene="cyoD"
                     /locus_tag="ACHOTW_001177"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP9843330.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(12054..12668)
                     /gene="cyoC"
                     /locus_tag="ACHOTW_001178"
     CDS             complement(12054..12668)
                     /gene="cyoC"
                     /locus_tag="ACHOTW_001178"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP9843331.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(12658..14649)
                     /gene="cyoB"
                     /locus_tag="ACHOTW_001179"
     CDS             complement(12658..14649)
                     /gene="cyoB"
                     /locus_tag="ACHOTW_001179"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP9843332.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(14671..15618)
                     /gene="cyoA"
                     /locus_tag="ACHOTW_001180"
     CDS             complement(14671..15618)
                     /gene="cyoA"
                     /locus_tag="ACHOTW_001180"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP9843333.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(16078..17553)
                     /gene="ampG"
                     /locus_tag="ACHOTW_001181"
     CDS             complement(16078..17553)
                     /gene="ampG"
                     /locus_tag="ACHOTW_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP9843334.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(17597..18175)
                     /gene="yajG"
                     /locus_tag="ACHOTW_001182"
     CDS             complement(17597..18175)
                     /gene="yajG"
                     /locus_tag="ACHOTW_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9843335.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            18480..18797
                     /gene="bolA"
                     /locus_tag="ACHOTW_001183"
     CDS             18480..18797
                     /gene="bolA"
                     /locus_tag="ACHOTW_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP9843336.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            19141..20439
                     /gene="tig"
                     /locus_tag="ACHOTW_001184"
     CDS             19141..20439
                     /gene="tig"
                     /locus_tag="ACHOTW_001184"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9843337.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            20685..21308
                     /gene="clpP"
                     /locus_tag="ACHOTW_001185"
     CDS             20685..21308
                     /gene="clpP"
                     /locus_tag="ACHOTW_001185"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9843338.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            21434..22708
                     /gene="clpX"
                     /locus_tag="ACHOTW_001186"
     CDS             21434..22708
                     /gene="clpX"
                     /locus_tag="ACHOTW_001186"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP9843339.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            22896..25250
                     /gene="lon"
                     /locus_tag="ACHOTW_001187"
     CDS             22896..25250
                     /gene="lon"
                     /locus_tag="ACHOTW_001187"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP9843340.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            25441..25731
                     /gene="hupB"
                     /locus_tag="ACHOTW_001188"
     CDS             25441..25731
                     /gene="hupB"
                     /locus_tag="ACHOTW_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP9843341.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            25923..27794
                     /gene="ppiD"
                     /locus_tag="ACHOTW_001189"
     CDS             25923..27794
                     /gene="ppiD"
                     /locus_tag="ACHOTW_001189"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9843342.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            27945..28316
                     /gene="ybaV"
                     /locus_tag="ACHOTW_001190"
     CDS             27945..28316
                     /gene="ybaV"
                     /locus_tag="ACHOTW_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9843343.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            28422..28820
                     /gene="fadM"
                     /locus_tag="ACHOTW_001191"
     CDS             28422..28820
                     /gene="fadM"
                     /locus_tag="ACHOTW_001191"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP9843344.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(28872..29567)
                     /gene="queC"
                     /locus_tag="ACHOTW_001192"
     CDS             complement(28872..29567)
                     /gene="queC"
                     /locus_tag="ACHOTW_001192"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP9843345.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(29632..31332)
                     /gene="ybaE"
                     /locus_tag="ACHOTW_001193"
     CDS             complement(29632..31332)
                     /gene="ybaE"
                     /locus_tag="ACHOTW_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP9843346.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            31432..32250
                     /gene="cof"
                     /locus_tag="ACHOTW_001194"
     CDS             31432..32250
                     /gene="cof"
                     /locus_tag="ACHOTW_001194"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP9843347.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            32402..32860
                     /gene="decR"
                     /locus_tag="ACHOTW_001195"
     CDS             32402..32860
                     /gene="decR"
                     /locus_tag="ACHOTW_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP9843348.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            32890..34662
                     /gene="mdlA"
                     /locus_tag="ACHOTW_001196"
     CDS             32890..34662
                     /gene="mdlA"
                     /locus_tag="ACHOTW_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9843349.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            34655..36436
                     /gene="mdlB"
                     /locus_tag="ACHOTW_001197"
     CDS             34655..36436
                     /gene="mdlB"
                     /locus_tag="ACHOTW_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9843350.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            36617..36955
                     /gene="glnK"
                     /locus_tag="ACHOTW_001198"
     CDS             36617..36955
                     /gene="glnK"
                     /locus_tag="ACHOTW_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9843351.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            36985..38271
                     /gene="amtB"
                     /locus_tag="ACHOTW_001199"
     CDS             36985..38271
                     /gene="amtB"
                     /locus_tag="ACHOTW_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP9843352.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(38320..39180)
                     /gene="tesB"
                     /locus_tag="ACHOTW_001200"
     CDS             complement(38320..39180)
                     /gene="tesB"
                     /locus_tag="ACHOTW_001200"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP9843353.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            39398..39970
                     /gene="ybaY"
                     /locus_tag="ACHOTW_001201"
     CDS             39398..39970
                     /gene="ybaY"
                     /locus_tag="ACHOTW_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP9843354.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(40003..40314)
                     /locus_tag="ACHOTW_001202"
     CDS             complement(40003..40314)
                     /locus_tag="ACHOTW_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP9843355.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            40477..40573
                     /gene="ffs"
                     /locus_tag="ACHOTW_001203"
     ncRNA           40477..40573
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHOTW_001203"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            40693..41046
                     /gene="ybaA"
                     /locus_tag="ACHOTW_001204"
     CDS             40693..41046
                     /gene="ybaA"
                     /locus_tag="ACHOTW_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP9843356.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(41088..42638)
                     /gene="pdeB"
                     /locus_tag="ACHOTW_001205"
     CDS             complement(41088..42638)
                     /gene="pdeB"
                     /locus_tag="ACHOTW_001205"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP9843357.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(42802..43272)
                     /gene="ylaC"
                     /locus_tag="ACHOTW_001206"
     CDS             complement(42802..43272)
                     /gene="ylaC"
                     /locus_tag="ACHOTW_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP9843358.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(43388..43939)
                     /gene="maa"
                     /locus_tag="ACHOTW_001207"
     CDS             complement(43388..43939)
                     /gene="maa"
                     /locus_tag="ACHOTW_001207"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP9843359.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(44111..44329)
                     /gene="hha"
                     /locus_tag="ACHOTW_001208"
     CDS             complement(44111..44329)
                     /gene="hha"
                     /locus_tag="ACHOTW_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP9843360.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(44355..44729)
                     /gene="tomB"
                     /locus_tag="ACHOTW_001209"
     CDS             complement(44355..44729)
                     /gene="tomB"
                     /locus_tag="ACHOTW_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP9843361.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        11322 a        11074 c        11844 g        10778 t
ORIGIN      
        1 taatgtcttt ttcttctgtt gattttgctt ctgtttttaa tatcggatta aaaaggtcgg
       61 tacggtaaag tttcgtgcct ttcatcctga cgatgtcagg aagaatagtg tttgaaagat
      121 tagcctcgct aaagtccaca ttttggcaat cactttctga taaatctgcc caattgaaag
      181 tggttttcac acaggagcag ttagttagaa tcatcccttt cgtttcattc ccatctgatt
      241 tctgataagt tatcctttgt attatttgat ctgcttctgg atattgcatc tcaggttctt
      301 tttctttacc atatatcatc gcattatcaa gaattgcgcc agtgcaattc atatttgtca
      361 ggtcgcattt cttaaatcta caattgttaa aattcgcatt tgtacaattc gcatttgtaa
      421 acttgacgcc ataaaaataa gtatgttcga agttgacatt tgagcagtta cagtttccca
      481 tatctatacc gaaatctata tcgggatctt tattgggatc tttaccggaa atattgctgt
      541 gtgctagata tagaccgctg aaatcgtaat atccattgtc atccttttct attgggagtg
      601 aataagtctt tactacctcg ttaacaatag agatagttcc acgtatcatc agattttctc
      661 cgtctttata aaaaataatg ttatgtttga tggtactgtt gtatggctct ggcaatgagg
      721 atgcaatttt attccagaaa caacaaagcg tggtctgttt ttgtttgggc gagaggcgat
      781 caaaatccag ctttaaatat gagtctaaat ctgacgttgc atctatatta aagttgggat
      841 ctggagttaa cgctgaatta taatttagaa ctgacaatgt attgatgtat ttatgaatcg
      901 atttaaatgc cttttgttta tcactgtcca gaaaaaaatc acgaattttg ttccacagcc
      961 tttcaataag attttgattt aaatcttgct tcattgagtt tcttaaatct gcaaccggta
     1021 catcagtaaa caggatcggt tgtttggtga tggttttcat catgattgta atcctcttac
     1081 gttgactaaa taagacagtt ttaggggata ttacctggag aaactatcaa aaatcgctca
     1141 aagggatgaa gacgatgaat agctgcaaac tcattcacga agaatttctc ttgcagaggt
     1201 ggattatggt tacagaaggt gatgggaggt acgttgtccc catcatcgca gacgatgggg
     1261 aattcagcag ataaaatggg tgaaaagcga aatttactta aatcctacga ccggatgcgg
     1321 tttatacggt gtttccagtt cagcaatctg ttccggcttc aaagtgatat ccaccgcgtt
     1381 cagcagctca tcaagctgtt cttcccgcga agttccgata atcggtgcgg caatgcccgg
     1441 tttactcaac aaccaggcca gcgcaacttg tgctcgtgtt gcaccgagtt cttcactgac
     1501 gcccgttaac cgctctgcga tctgcgcgtc attttcatcg ctttctttat agagattttt
     1561 ccccacctca tcagacacca gtcgtgcggt agtttctccc cacggacgcg tcagtcgccc
     1621 ccgcgccagc ggactccacg gaatgaccgc cacgccttcc tgataacaca gcggcagcat
     1681 ctcgcgctct tcttcgcgat aaatcagatt gtagtgatcc tgcatactga caaactgcgc
     1741 ccagccgtgc tgtttttgta gttccagtgc ctgagcaaac tgcgaagcgt gcatggatga
     1801 cgcgccgata taacgcgctt tcccggcttt taccacgtcg ttgagggctt ccagcgtctc
     1861 ttcgatcggc gtgttgtaat cccagcgatg aatttgcagg atatcgacat aatccatgcc
     1921 gagacgtcgc aggctgtcgt cgatagagcg caaaatttgt gcacgggata atccttccgg
     1981 taaatcacca acgcgatgga acactttggt ggcaacgacc acgtcttcac gacgggagaa
     2041 atcccgcagt gcgcgaccga cgatctcttc gctgctgcca tcggaatagc tattggcggt
     2101 atcaaagaaa tttatgccgc cttccagtgc gcgtttaatg atgggacggc tgctctcttc
     2161 cggcagtgtc catgcgtgat taccacgatc tggctcgcca aaggtcatac agccgaggca
     2221 aagtcgggaa acgcgaaggt cggtttttcc taaggggttg tattgcatgc tgccactcct
     2281 gccatgaata atttcttaag catagcagga gtggagtagg gattatgcca gccaggcctt
     2341 gattttggct tccataccgg cggcatcgag gccgagttcg gcgcgcattt cttcctgagt
     2401 tccttgcgga ataaagaagt ccggcaggcc aatgttcagc acgggtactg gtttacgatg
     2461 ggccatcagc acttcgttca ctccgctgcc tgcaccgccc ataatggcgt tttcttctac
     2521 ggtaaccagt gcttcatgac tggcggccat ttccagaatt aacgtttcat caagcggttt
     2581 cacaaaacgc atatcgacca gcgtggcgtt cagcgattcg gcgactttcg ccgcttctgg
     2641 catcagcgta ccaaagttaa ggatcgccag tttctcgcca cgacgcttca caatgccttt
     2701 gccaattggt agtttttcca gcggcgtcag ttccacgccg accgcgttgc cacgcgggta
     2761 gcgcaccgct gacgggccat cgttatagtg atagccggta tagagcatct ggcgacattc
     2821 gttttcatcg ctcggggtca taatgaccat ttccggtatg cagcgcaggt aagagagatc
     2881 aaaagcgccc tggtgggttt gaccgtcagc accaacaatg cccgcgcggt cgatggcgaa
     2941 caggaccggg agtttttgaa ttgccacgtc atgcagcacc tgatcatagg cgcgttgcag
     3001 gaaggtggag taaattgcca cgatcggttt gtagccacca atcgccagac cggcggcaaa
     3061 ggtcaccgcg tgttgctcgg caatcgctac atcaaaatag cgatccggga atttacgtga
     3121 aaactcgacc atgccggaac cttcacgcat cgccggagta atcgccatca gtttgttatc
     3181 tttcgctgcc gtttcgcaca accagtcgcc aaagattttt gaatagcttg gtaaaccacc
     3241 gctacttttc ggcaaacaac cgctggaggg atcaaattta ggcacggcgt ggaaggtgat
     3301 tgggtctttt tctgccggtt cataaccacg accttttttg gtcatgatat gcaggaactg
     3361 cgggcctttc aggtcgcgca tgttcttcag cgtggtgata agccccagca catcgtgacc
     3421 gtcaaccggg ccgatgtagt taaagcccag ctcttcaaac aacgtgccag gcaccaccat
     3481 gcctttaata tgttcttcgg tacgtttgag cagctcttta attggcggaa cgccagagaa
     3541 aacttttttc ccgccttcgc gcagtgaaga gtaaagctta ccggaaagca gctgcgccag
     3601 atggttgtta agcgcgccaa cattttcgga aatcgacatt tcattgtcgt tgaggaccac
     3661 cagcatatca ggacgaatat cgcccgcgtg attcatcgct tcaaacgcca tgcctgcggt
     3721 aatcgcgccg tcgccaatca cacagacggt gcggcgattt ttgccttctt tctcggcagc
     3781 aaccgcaata ccaattccgg cactgatgga ggttgatgaa tgcccgacgc ttaatacgtc
     3841 atattcgctt tcgccgcgcc acgggaacgg gtgtagaccg cctttctgac ggatggtgcc
     3901 gattttgtcg cggcgtccgg tcaaaatttt atgcggataa gcctgatgcc ccacatccca
     3961 aatcaattgg tcaaacgggg tgttatagac atagtgcagc gccacggtca gttcgaccgt
     4021 gcccagcccg gaggcgaagt gcccgctgga acggctcacg ctgtcgagta aatagcggcg
     4081 cagttcgtcg cagagtttcg gtaaactctc ttttggcaac agtcgtagct cctgggtgga
     4141 gtcgaccagt gccagggtcg ggtatttggc aatatcaaaa ctcatcaggg gcctattaac
     4201 acttattgtt tatttattac gctggatgat gtagtccgct agcgcttcca gtgccgaagt
     4261 atcgatcgac tgttcagcca gttgtttcag cgactggcgg gcatcgtcga tcagatcctg
     4321 ggctttcttc cgggcttgct caagacccag gagtgcaggg taggtacttt tgccaagctg
     4381 ctggtcggca ccctgacgtt ttcccaacgt tgcagtatct cccaccacat ccaggatgtc
     4441 atcctgaacc tggaaggcaa ggccgatgct ctctgcgtac ttgtcgagta ctggtagagc
     4501 acgacgtcct ttatctccgg cgcttaatgc accaaggcga acggcggcgc gaattaatgc
     4561 gcctgtttta tgacgatgaa tacgctcaag cgcgtccaga ggtacgtgtt tgccttccgc
     4621 gtctaaatcc aacgcctgac caccacacat tccggcaata ccgctggcgc tcgccagttc
     4681 agaaatcatc gaaattctgt cgcgatccga cacttctggc atatcagcat cgcttaaaat
     4741 cgagaacgcc agcgtttgta aagcgtcgcc agcgagaatc gcgttcgctt cgccaaactt
     4801 cacatggcag gtcggcaagc cgcgacgcag atcgtcatca tccatcgccg gtaaatcatc
     4861 atgaattaat gagtaagcgt ggatacactc tacggcggca gcgggtgcgt ccagcgtgtt
     4921 tgtgctaacg ccgaacatat gaccggtggc ataaaccagg aaaggtcgca ggcgcttacc
     4981 acctaataat gcgccatact gcatggtttc gaccacggga gtgttctgaa agggcagtgg
     5041 ggcgataaaa cggctcagcg cctggttggc ctgcttaacg caggcttcga gttgctgcgg
     5101 aaagtccatt actcattgtc cggtgtaaaa ggggttagag aggcgtcttc gttgtcagac
     5161 agcagaattt gtacgcgctg ttcggcttgt tgtaatttgg cctgcccctg acgtgccagc
     5221 tgcacgccgc gttcgaactc gttcagcgcc tcttccagcg gcaggtcgcc actttccaga
     5281 cgggttacaa tctgttccag ctcgctcagc gccttttcaa agctggcggg cgcctcattt
     5341 ttcttcggca taatgaatgt ctgactctca atatttttcg ccccgtcatg gtaacggact
     5401 cagggcaaat agcaaataac gcgcaatggt aaggtgatgt gcgcagcaaa gcgatgttag
     5461 tggtatactt ccgcgcctgg atgcagccgc aggtgtgggc tgctgtattt ttccctatac
     5521 aagtcgctta aggcttgcca acgaaccatt gccgccatga agtttatcat taaattgttc
     5581 ccggaaatca ccatcaaaag ccaatctgtg cgcttgcgct ttataaaaat ccttaccggg
     5641 aacattcgta acgttttaaa gcactatgat gagacgctcg ctgtcgtccg ccactgggat
     5701 aacatcgaag ttcgcgcgaa agatgaaaac cagcgtctga ctattcgcga cgccctgacc
     5761 cgtattcctg gtatccacca tattctcgaa gtcgaagacg tgccgtttac cgacatgcac
     5821 gatattttcg agaaagcgtt ggttcagtat cgcgatcagc tggatggcaa aaccttctgc
     5881 gtacgcgtga agcgccgtgg caaacatgat tttagctcga ttgatgtgga acgttacgtc
     5941 ggcggcggtt taaatcagca tattgaatcc gcgcgcgtga agctgaccaa tccggatgtg
     6001 actgtccatc tggaagtgga agacgatcgt ctcctgctga ttaaaggccg ctacgaaggt
     6061 attggcggtt tcccgatcgg cacccaggaa gatgtgctgt cgctcatttc cggtggtttc
     6121 gactccggtg tttccagtta tatgttgatg cgtcgcggct gccgcgtgca ttactgcttc
     6181 tttaacctcg gcggcgcagc gcatgaaatt ggcgtgcgtc aggtggcaca ttatctgtgg
     6241 aaccgctttg gcagctccca ccgcgtgcgt tttgtcgcta ttaatttcga gccggtagtc
     6301 ggggaaattc tcgagaaaat cgacgacggt cagatgggcg ttatcctcaa acgtatgatg
     6361 gtgcgtgccg cgtctaaagt ggctgaacgt tacggcgtac aggcgctggt caccggcgaa
     6421 gcgctcggcc aggtgtccag tcagacgctg accaacctgc gcctgattga taacgtctct
     6481 gatacgctga tcctgcgtcc gctgatctct tacgacaaag agcacatcat caacctggct
     6541 cgccagattg gcaccgaaga ctttgcccgt actatgccgg aatactgcgg tgtgatctcc
     6601 aaaagcccga cggtgaaagc ggttaagtcg aagattgaag cggaagaaga gaaattcgac
     6661 ttcagcattc tcgataaagt ggttgaagaa gcgaataacg ttgatatccg cgaaatcgcc
     6721 cagcagaccg ggcaggaagt ggtggaagtg gaaaccgtca atgacttcgg cccgaacgac
     6781 gtgattctcg atatccgttc tgtcgatgaa caggaagata agccactgaa agtcgaaggg
     6841 attgatgtgg tttcactgcc gttctacaag ttgagcacca aatttggcga tctcgaccag
     6901 aacagaacct ggctactgtg gtgtgagcgc ggggtaatga gccgcctgca ggcgctctat
     6961 ctacgcgaac agggctttaa gaatgtgaag gtgtatcgtc cgtaatttgt ggtttttacg
     7021 tcgcatctgg tcagatgcga cgtttgccgc atccgacact actcgtaata attataaatc
     7081 cctgccgcca tcaccagttg tgatgccact tcatgggctt tctcgcgccc aaccagcagg
     7141 tcgataattt tcagaccaaa gtcgatagct gtacccggcc cctggctggt cagcaatttt
     7201 acccgtgcat cccagacgac gcgcttgtcc tgccattgtt cagcgggaat tttgtctttc
     7261 agcgtcggga agccggtcat attgccaatc ggaaagatat cgtgcggcac cagcacggtg
     7321 gctggcgcgg cgcaaatagc cgcgacgata cgcccggaac ggtggaactg tttaacggtt
     7381 tcaaccagca gggtactatc gcgaaaacac tccgcgcctt taatgccacc aggcagcacg
     7441 atcacgtcat attcgccatc agccacttcg accagcggcg catccgccag cagcttcacg
     7501 ccgcgcgagc aggtaatcgc caggttgcca tcgctggcta cgctggcagt ggtgactttg
     7561 ataccgccgc gaatcaagag gtcgatcgtg gtgacggctt cagtctcttc actaccaggg
     7621 gcgaggcaaa ccagtgccga tgcgctcata ttcactctcc tttcttttta ccatttcaaa
     7681 caggcgggtg ttttccggta cggcaatccc atgcgcgcgg gcgcggcgta agagaaaacc
     7741 attgatatag tcgatttcgg tgtggcgcag cgtgcggata tcctgcaaca tcgacgagat
     7801 attttccgct gtggcatcaa tcacctgcat cacgtaatca cgtaaatctt ccgctgaagt
     7861 atgatgccct tcgcgttcga tcactgccgc gacttcttcg catatttgca taatttcttg
     7921 cggatgatga cgtaattcac cgttcgggca attccagatg gcagtcagtg gattaatcac
     7981 acagttgact gccagcttgc gccacagctc ggcgcgaata ttgttatgcc aggcaacgtc
     8041 aggcaacacg gtttgcaata tatccgccag ataactgtaa tcgccgtcct gttgccgtgc
     8101 tggaccaata tgcgtgatac cgtttgccac atgaataatg acattgccgt cgcggcaggc
     8161 ggcatgggtg gtggtgccca tcagtaatgg ctgctgaatg ttttgcaatt cttcgatggt
     8221 gcccatgccg ttgtgaatta acagtattgg cgtagttaca ggcagtgtgg acgcgaggct
     8281 tttgacggca tcggaaacct gccatgcttt cagcgtcacc aggagcagat cgctggtggc
     8341 aagaaaatcg ggatcgttgg cggtcagcga ttcgttaaat atcgaaccat cagtctcaac
     8401 cagattcaca ctacaataag gttgcggtac gcgcagccag ccctgaactt cgtgaccctg
     8461 tttacaaagt gctgtaagcc ataattgccc taaggcaccg catcccaata cggtaatttt
     8521 cattgttcct cctcacccgc aaccactccg ggtgttcaat aaggctatcc cttaattgtg
     8581 catgctgttg cgactatgca caattaaggg atacgtcctg gtgcaggact gttggttatt
     8641 taactttgca ggtattatgc ttcgcatcaa aaatgaaggg agaggaaaag atgccatctt
     8701 tcgatattgt ctctgaagtt gatcttcagg aagcacgtaa cgcggtcgat aacgcgagcc
     8761 gcgaagtgga gtcccgtttt gacttccgta acgttgaagc ctcatttgag ctgaacgacg
     8821 ccagcaaaac catcaaagtg ttgagcgagt ccgacttcca ggtcaatcag ttgctggata
     8881 ttctgcgtgc caagctgctg aagcgcggca ttgaaggcag ttcactggat gtaccggaaa
     8941 atatcgttca tagcggtaaa acctggtttg tggaagcgaa actgaaacag ggcattgaga
     9001 gcgccactca gaaaaaaatc gtcaagatga tcaaagacag caaactgaaa gtgcaggcgc
     9061 aaattcaggg cgatgagatc cgtgtgacgg gcaaatctcg tgatgattta caggctgtca
     9121 tggcgatggt gcgcggtggc gatctcggtc agccgttcca gttcaaaaac ttccgcgatt
     9181 aatcgcgaca acctgcgctt tgttcatgtc agatgcggca tgaacgcctg gttcggccta
     9241 caaagttttg taaatcagga acttagtagg cctgataagc gtagcgcagc aggcaatttc
     9301 gcctcgcttt tatgcctggc gaattgcctg ttcaacatca aagcgattcg tcactttgct
     9361 gtcgattttc acataagctg aatgttcttc ttctgcaatc aacacttctt tgatgccttc
     9421 agtttccagc aaacgcactt ttaacgcctc gtttgcggca atgttcgccg ggatttcaat
     9481 gcgcaaactg ctgacatacg gcggttcttt catggtactg gcgactgcca gccacactgc
     9541 ggccagcatt gcgccagcga gaaatacccc ctgaccgtca aacatgccgt caatccagcc
     9601 gcccagcgaa ccgccaatcg ccacgccaag aaactggctg gtggagtaaa cacccatcgc
     9661 cgtacctttg taacctgctg gcgactcttt actgataagt gagggcagga gggcttccat
     9721 caaattaaac gccacaaaga aaagctgcac gccgaccacc agttgccaga actgcgtttg
     9781 cgcgttcctc aacacaattt ccgcaaccac gatcaacccg acgcagaaga caaagacttg
     9841 cttcattttg cgcttaactt cagcgtagat aatgaaaggc acgaccgagc caaaggcgat
     9901 tagcattgtc gccagataga ccttccagtg ttcagccgcc gggaaccccg catcagccag
     9961 ttgtccgggc agggcaacaa acgtcgacat cagcaaaata tgcagacaca taatgccaaa
    10021 gttgagtttc agcagccgcg gttccgccag caccttactg aaactgcctt tcaccattcc
    10081 ggactcacga ttaagtacgt gagtgctact gttgggcaca acccaaatgg tcaacgcaat
    10141 gccggtcgtt gccagaatag cgatcatcca gaacagcgcg tgcagcccaa gtttgtgagt
    10201 gatgatcggg ccaagcacca tcgcaatggc aaaggtaatg ccaaagctca cgccgataaa
    10261 cgccattgct ttggtgcggt tttgttcgcg cgtgagatcg gaaagcagcg ccataacggc
    10321 agcggcaatc gcaccggagc cttgtagcgc ccgacccaga ataattcccc agatggagtc
    10381 agagagcgca gcgataacgc taccggcggc aaacactgcc agcccaccga caattaatgg
    10441 tttgcgacca atgcggtcgg aaagcaggcc aaacggaatc tgaaaaacgg cctgagtcag
    10501 accataaata ccaatggcaa taccgattaa tgcttcgctg gcaccttgca gagccatgcc
    10561 gtacgtggtc agaaccggca gaaccatgaa catgcccagc atgcgcaacg agaatacggt
    10621 ccctaaaccc caggtcgcgc gccgctcacc tggcgtcatt ttataatcgt tcattaccac
    10681 ctctgtttta aattcgcgac tagtgtaaag cggcaaacga atggggtaaa catgcggatt
    10741 ttttagtaaa tatttcgaga gggatatgtt tctaatgcta agaaaaaagg tgccgtagca
    10801 cctttttaat agagaggttg tgttaccaca cagcagccag cagcgtatgc gagtccggca
    10861 ccataaaatc aacggacatc atcaccgaga gggcagtgat ggcgatgata gagaagccga
    10921 acagcttgcg cgcccagatt ctgtcatcag caactttata accgcgcaga gccataccta
    10981 accaccagac gctaaccgcc gcggcgacca ccagatattt atatccagcg taaccgccaa
    11041 gagagagcat cagcgtggca acggcaaagg cgatgatata cagcgtgatg tgattcttcg
    11101 ccaccgaaat gccttttacc actggcaata ccggaatgtt tgccgcctgg taatccttaa
    11161 agcggaaaat ggcgatggca taggagtgag gcatctgcca caggctgaag atagccagca
    11221 ggatcgctgc gccgctatcg aactcgccgg ttactgcaca gtagccaatc accggcggcg
    11281 cagcgccgga gagcgaacca atcaatgtgc cgtagacgga gtggcgtttc atgtacaggc
    11341 tataaacgcc gacataaacc acaaagccca tcacccccag ccagcaggcc agcggattcg
    11401 cgccaaacca cagcagcata aagccagcaa tacccagcaa ggtggcgtac accagcgaga
    11461 cagcaggaga gatcaggcct ttaaccagca cccgattctt cgtcctttcc atctttctgt
    11521 cgatatccct gtcgatgtag ttgttaaaca cacaacccga cgccacaacc agtgacaccc
    11581 caaccagcgt gtagataaac aggggataat caatgctgcc ctttgaggcc agcaggaatc
    11641 ccccaatcac cgagatcagg ttgccaaaga tgatgcctgg tttcgttact tgcaggtatt
    11701 gcttaaacat cataaccgcc gctcttagtg catcatcatg ttgtagttga ggttccacat
    11761 aatccagatg gagcctacaa ctaggatagc gatgattagc acggtgaaga caaacgccgt
    11821 catgttccag ccttcatctg atttggtatt catgtgcagg aagcacacca gatgcaccag
    11881 aacctgtacc actgccattg ccaggattgt tcccagaatt acggccggag aggcagcccc
    11941 tgtcatcacc atccagaacg gaatcaccgt cagaatgatc gacaggataa agcctgtcat
    12001 gtaggttttt acgctgccat gggacgcgcc gctgtgatcg gtagaatgac tcattacatc
    12061 gcccccatca gataaacaac agtgaacaca cagatccaaa ccacatccag gaagtgccag
    12121 aacaggctca ggcacatgat gcgggtacgg ttagtgctgg tcaggccgcg acgagcgatt
    12181 tgtaccatca gcaccgccat ccagataaga ccggaagtga cgtgcagacc gtgcgtgccg
    12241 accagcgcaa agaacgctga caggaagccg ctgcgatccg gacccatgcc gttaacaatc
    12301 aggtgatgga attcgtagat ttccatcccg ataaatccgg cgccaaacaa ccaggtcaac
    12361 gccagccagg agataacctg gcttttgttg tttttgtaca tggcgatagc cgccatgccg
    12421 taggtgatgg agctgaacaa cagcaagaaa gtttcaacca gaacgaacgg cagttcgaaa
    12481 atgtccttac ctgtcgggcc gcctgcggtg ccgttcacca gaacggcata ggtagcaaac
    12541 aagatagaga acagaatgca gtcgctcatc aggtagatcc aaaatccgaa gattttggtt
    12601 ccgcctgcat cgtggtgccc gtgttcgtgc gcgtggacag tcgcgtgcgt caaagtatca
    12661 gttgccattt ttcagccctg ccttagtaat ctcatcgaaa tgctggtttt ccagtttttc
    12721 gatttctgcc accggcacgt agtaatccac gtcctcgtcg aagcttttca cgatccaggt
    12781 gatgatcatg cctgcgaagc caacaatcgc cagccaccag atatgccaga tcatggcgaa
    12841 accgaagatg gtggagaaag ccgcaatgac gatacctgca ccgctgtttt tcggcatatg
    12901 aatttcttca tagtggtcag gctttttata cgcttcgcct ttctctttca tttcccagaa
    12961 tgcatcacgc tcgtgaacgt gcggcacaac ggcaaagtta tagaacggag gcggggaaga
    13021 ggttgcccac tccagcgtac ggccacccca cgggtcgcca gtcaggtcac ggttctggtc
    13081 gcggtcacga atagaaacgt acatctgaat aacgaggcag agaataccta gcgcaatcag
    13141 tactgcaccg ctggctgcaa tcatcagcat ggtgtggaac tgcgggtcaa tctgctggct
    13201 caaacgacgg gtcatgccca tgaagcccag cgcgtacagt ggcataaagg caacgaagaa
    13261 gccgatgatc cagaaccaaa acgcgcgttt accccaggtt tcgttcagtt tgaaaccgaa
    13321 cgctttaggc caccagtagg tcatccctgc gaagcagccg aagaccacgc cgccgatgat
    13381 cacgttatgg aagtgcgcaa tcaggaacag gctgttatgt agaacgaagt ccgcgcccgg
    13441 tactgccagc agcacgccag tcataccgcc caccgagaag gtgacgataa aaccgatagt
    13501 ccacagcatc gcagaatgga acacgatgcg gccctgatac atggtgaaca gccagttgaa
    13561 gatcttcacc ccggtcggga tggcgataat cattgtggtg ataccaaaga aggcgtttac
    13621 gttcgcgccc gcacccatcg taaagaagtg gtgcagccaa acgatgaacg acagcacggt
    13681 gatacagacg gttgcccata ccagcgaggt ataaccaaac agacgtttac gcgagaaggt
    13741 tgccgcaatt tcggagaaca caccgaaaac aggcaggatc aggatgtaaa cttccgggtg
    13801 gccccaggcc caaatcaggt tgatgtacat catcatgttg ccacccatat cgttggtaaa
    13861 gaaatgggtg cccagatagc gatccagggt caacaacgcg acggtaaccg tcagaattgg
    13921 gaaggaagca ataatcagta cgttcgcgca cagtgatgcc caggtaaata ctggcatctt
    13981 gaacatggtc atgcccggtg cgcgcatctt cagaatggta acgaagaagt tgataccggt
    14041 aagcgtcgta cctataccgg atagctggag actccatatc cagtaatcga caccgactcc
    14101 cggactgtac tctattcccg atagcggtgg ataggccagc cagccggtct gcgcaaattc
    14161 gcccacgccg agagaaacgt taaccagaat cacaccaaca acggtaaacc agaagcttaa
    14221 gttgttgagg aacgggaacg caacgtcacg cgcgccgatc tgcagcggaa ccaccaagtt
    14281 catcagaccg ataacgaaag gcatcgccac gaagaagatc ataatcacgc cgtgcgcggt
    14341 gaagatctga tcgtagtggt gaggtggcag gaagcccgct tcgcccgccg aggcaagagc
    14401 ctgctggcta cgcatcataa tggcgtcagc aaaaccacgc agcaacatca caatcgccac
    14461 gatgatatac atgataccga ggcgtttatg gtcgacggag gtcagccact ctttccacag
    14521 gtaggtccac ttaccgaagt aagtgatcag gccaacgagc gccagacctc ccaaaataat
    14581 gccagcgatc gtaaccatga cgataggttc atggaacggg actgcatcaa gtgataattt
    14641 tccgaacatc tttattcttc ctcaacccct ttaatgggcg gattccgcgt ggctcatgtc
    14701 catgccttcc ataccttcgt gtgcgctgtg ctcaccttct ggctgggtca tgtccatgct
    14761 cttaccgtga gccataaact tgttaattac atcggcaaac aagtttggtt tcacgttgga
    14821 gaaatattcc acctggttgt attcgctagg cgcggccagt ttttcgaacg ctgccatgtc
    14881 agacatggag ttcggcgact gtttcgcttt tgcgacccac tggtcgaatg cggcgcgatc
    14941 cggtgttgca atagctttga acttcatgcc tgagaagccc gggccgctgt agctggcgga
    15001 gataccgtca taagtgccgg gttcgttggc gatcagatgc aggcgagtct gcataccggc
    15061 catggcataa atctggctac ccagacgcgg aatgaagaag gagttcatca cggagttgga
    15121 ggtcactttg aagtacaccg gagtgttcgc cgggaaagcg atttcattca cggtagcaaa
    15181 gccctgttcc gggtagatga agaaccattt ccagtccatg gaaaccactt cgatggtaat
    15241 gggcttctcg tcgtgtgcca gcggcttgct aggctcaaga gcgtgagtgg ttttccaggt
    15301 cagtaccgca aggaagatga tgattaagat aggtaccgtc cagaccacag cttccacttt
    15361 attggagtgt gaccagttcg ggctgtactt agcatcttta ttgctcgcac ggtacttcca
    15421 ggcgaaacca acagccatca agattgcggg aataacgaca atcaacatca ggccaaatgc
    15481 cgtcagtatc agtgaacgtt gctccagacc aatctgtcct ttgggatcta acagcgcaga
    15541 attacagcca ctgagcaata cagtgcctgc aaataatgac aaccatccca aacttttatt
    15601 gtatttcctg agtctcattt aacgacctca attccacggg atctggtggc gtttaaaatg
    15661 tgtgggcatt ttacgggaag gttacattac tgtaaacatg attaaattcc tgttacttgg
    15721 atttggctgt caggtcacat atgttgcaaa acatatcagg ttgattttaa gaagctgttg
    15781 caaaagggcg ttataacaaa aggggagtgc agaagaagtc tggacgggca attggtataa
    15841 tcaatgcgaa ataaaacaat tatttaacaa ataattatca ttggagtgac aacttggtgg
    15901 tgagaaaata aagactcatt agctgaatcg tgtaagaaaa atagaattta taaatggcgc
    15961 caataatttc caaacgctaa gccgcacaaa agaacaaata ttaataagcg atgctgatgt
    16021 ttatttagcc gtaataatta cggcgagtga ttactacagc taaataatat ttacagatta
    16081 cgtcagatgc gtttttcgta gcgccagata atccagcaaa ccaccaagca cgacgccaga
    16141 aagggcgact aaaaccccga cttccagcag agcaggcagg aaagagaagt gcgtcaaatc
    16201 cagcgcgtcc atcgtcaata gcagtaacca cacggccaac aggctgacgc ccgccgccag
    16261 tgtccacatg gcaaaggcat aacctgccgg atatgcggta cgggagataa agttgtcatt
    16321 tactcgtgta tattcaagcg tctggcggca aaccagcagc aaaataagcc ctggtacggc
    16381 agcggcgacg gagaatagat agaatgtcga ccagccgtgt gcttcaacaa accaacccgc
    16441 cacggggccg acataaactc gccctacagc agaaagcgct gagagcaggg caaattgagt
    16501 agcggaaaat gacttattac atagcgtcat taacagcgcg acaaaggctg atgtgcccat
    16561 cccgccacag aggttttcga aaaagacggc tgcgcccatg ctgtagagat gcttatcagt
    16621 aatcgacagc agccagtaac cagcgttaga cgcaccttgt aaaatgccga aaatcagcag
    16681 tgcccggaac agtgacaggc gctgcatcaa aatcccaccg tacaatgcgc caacaatggt
    16741 cgctagtaag ccaagcgttt tgtttaccac gcctacttca cccgcatcaa acccgacgcc
    16801 gcgaatcaaa aacgtggttg tcaggctcat ggcgaatgcg tcgcccagct tatacagcac
    16861 gataagaagc aaaataagcc aggcattatt gcgaccaaag aaatctcgca gaggtgcaac
    16921 aaccgcttgt tccagcgttt tgggcacagg aatggtgtcg gttggttctg gtgcaagcaa
    16981 cgtcgcgata atacagggga tcaacagcgc cgccatcaac cagtacatgc cctgccagcc
    17041 cagccattta tctgccagcc acagggctag gccgccggaa accagcatcc ctaaacggta
    17101 acccagaacg ctgattgccg cgcccgcacc acgttcttct gccgggagca catcggtttt
    17161 ccacgcatcg aagacaatat cctgggaggc agagcaaaaa gcgatcacca ccgccagcgc
    17221 cgccatccag cggagttggg tgcctggttc gagaaaaccc atcgccgcaa tggcgactaa
    17281 taacaggatt tgcgtggcga gcagccaacc gcgccgccgc ccaaaaaatg ggggcgtgta
    17341 gcggtccatc agcggtgacc agaggaattt aaaaacgtaa gcctggccta ccagagagaa
    17401 gaaaccaatg gttttgagat cgatattctc gaccgtcatc caggcctgta aggtgccgga
    17461 tgtcagggcg agcggtagcc cggaagcaaa acccaggatc agcaatatgg ctgaacgcgg
    17521 ctgttgaaaa atacgtaaat attgactgga catgggcgtc tacaggcccg gcttgcaccg
    17581 ggccagaggg cagaaattaa cgcgcgttct gcttgatgaa ttcgtgaatg ctggtgtcct
    17641 gagacatatc agcgatggta tcggtcagca cactgttaac cgcatcggcg atatttttgt
    17701 tggaggcctg gaacgcacct tcaacgttgt agctggcacg atagtttttg gtcattttgt
    17761 tgccattctg cgcggtagcg atgatggcga tatccgcttt ggtagcgatg ttgtagcgca
    17821 cgttgccctg ggacacgtca gcatacagct ggctaacgat gatttgcaga ttaaccgggc
    17881 cattcggacc aaccatgtaa ccacgcgcgg tcatctgttt ttccagcact tcttgcagca
    17941 ggaaacgcag atcgcgggag gcggtcaggg taacgatttg attatcgcgg gtgacttttg
    18001 ccagcgcctg atcggtacgc tgatcggcac cattaatgct tacggtgacg cccatcaggc
    18061 ttggatcctg ctgtggcagt gtaatcgtcg gggaaacttc aatagttgtt ggcggtcttg
    18121 cgcatcctgc aagcataaac agagcaacta acgggaagag gatttttttg aacatgttcg
    18181 ggctctcaga gactcttaag cgtgtttggt aaaaattccc gccatcataa cattgccaac
    18241 ggcgagggga agtaggtaag gcatgtaaat tcatcatgtt gacgaaataa tcgcccctgg
    18301 taaaagaaac gctgatgcga ggcctgtgtt tcaatcttta aatcagtaaa cttcatacgc
    18361 ttgacggaaa aaccaggacg aaacctaaat atttgttgtt aagctgcaat ggaaacggta
    18421 aaagcggcta gtatttaaag ggatggatga catctcagcg ttgtcggagg agatatttca
    18481 tgatgatacg tgagcggata gaagaaaaat taagggcggc gttccaaccc gtattcctcg
    18541 aagtagtgga tgaaagctat cgtcacaatg tcccagccgg ctctgaaagc cattttaaag
    18601 ttgtgctggt cagcgatcgt tttacgggtg aacgttttct gaatcgtcat cgaatgattt
    18661 acagtacttt agcggaggaa ctctctacta ccgttcatgc gctggctctg catacttaca
    18721 ctattaagga gtgggaaggg ttgcaggaca ccgtctttgc ctctcctccc tgtcgtggag
    18781 caggaagcat cgcgtaaaaa cgcatttgca actgtcggcg cttttccagt atgttgctaa
    18841 agattttatg aaaaacggcc tgcgggccgt tttgttttgt ctggattttg cgctttttgc
    18901 ccagcattca gacgaaaatt gcccgggaat tgtgaaaaaa tacgcgacag cgcgcaataa
    18961 ccgttctcga ctcataaaag tgatgccgct ataatgccgc gtcctatttg aatgctttcg
    19021 ggatgattct ggtaacaggg aatgtgattg attataagaa catcccggtt ccgcgaagcc
    19081 aacaacctgt gcttgcgggg taagagttga ccgagcactg tgattttttg aggtaacaag
    19141 atgcaagttt cagttgaaac cactcaaggc cttggccgcc gtgtaacgat tactatcgct
    19201 gctgacagca tcgagaccgc tgttaaaagc gagctggtca acgttgcgaa aaaagtacgt
    19261 attgacggct tccgcaaagg caaagtgcca atgaatatcg ttgctcagcg ttatggcgcg
    19321 tctgtacgcc aggacgttct gggtgacctg atgagccgta acttcattga cgccatcatt
    19381 aaagaaaaaa tcaatccggc tggcgcaccg acttatgttc cgggcgaata caagctgggt
    19441 gaagacttca cctactctgt agagtttgaa gtttatccgg aagttgaact gcagggtctg
    19501 gaagcgatcg aagttgaaaa accgatcgtt gaagtgaccg acgctgacgt tgacggcatg
    19561 ctggatactc tgcgtaaaca gcaggcgacc tggaaagaaa aagacggcgc tgttgaagca
    19621 gaagaccgcg taaccatcga cttcaccggt tctgtagacg gcgaagagtt cgaaggcggt
    19681 aaagcgtctg atttcgtact ggcgatgggc cagggtcgta tgatcccggg ctttgaagac
    19741 ggtatcaaag gccacaaagc tggcgaagag ttcaccatcg acgtgacctt cccggaagaa
    19801 taccacgcag aaaacctgaa aggtaaagca gcgaaattcg ctatcaacct gaagaaagtt
    19861 gaagagcgtg aactgccgga actgactgca gaattcatca aacgtttcgg cgttgaagat
    19921 ggttccgtag aaggtctgcg cgctgaagtg cgtaaaaaca tggagcgcga gctgaagagc
    19981 gccatccgta accgcgttaa gtctcaggcg atcgaaggtc tggtaaaagc taacgacatc
    20041 gatgtaccgg ctgcgcttat tgacagcgaa atcgacgttc tgcgtcgcca ggctgcacag
    20101 cgtttcggtg gcaacgaaaa acaagctctg gaactgccgc gcgaactgtt cgaagaacag
    20161 gctaaacgcc gcgtagttgt tggcctgctg ctgggcgaag ttatccgcac caacgagctg
    20221 aaagctgacg aagagcgcgt gaaaggcctg atcgaagaga tggcttcagc gtacgaagat
    20281 ccgaaagaag ttatcgagtt ctacagcaaa aacaaagaac tgatggacaa catgcgcaat
    20341 gttgctctgg aagaacaggc tgttgaagct gtactggcga aagcgaaagt gactgaaaaa
    20401 gaaaccactt tcaacgagct gatgaaccag caggcgtaat ttacgcagca taacgcgcta
    20461 aattcgcaca aaggcccgtc accgccaggt ggtgggcttt tttttgtcat gaattttgca
    20521 tggaaccgtg cgaaaagcct ctttcggtgt tagcgtaaca acaaaagatt gttatgcttg
    20581 aaatatggtg atgccgtacc cataacacag ggactagctg ataatccgtc cataaggtta
    20641 caatcggtac agcaggtttt ttcaatttta tccaggagac ggaaatgtca tacagcggcg
    20701 aacgagataa ctttgcaccc catatggcgc tggtgccgat ggtcattgaa cagacctcac
    20761 gtggtgagcg ctcttttgat atctattctc gtctacttaa ggaacgcgtc atttttctga
    20821 ctggccaggt tgaagaccat atggctaacc tgattgtggc tcagatgctg ttcctggaag
    20881 cagaaaaccc agaaaaagat atctatctgt acattaactc cccaggcggg gtgatcactg
    20941 ccgggatgtc tatctatgac accatgcagt ttatcaagcc tgatgtcagc accatctgta
    21001 tgggccaggc ggcctcgatg ggcgctttct tgctgaccgc aggggcaaaa ggtaaacgtt
    21061 tttgcctgcc gaattcgcgc gtgatgattc accaaccgtt gggcggctac cagggccagg
    21121 cgaccgatat cgaaattcat gcccgtgaaa ttctgaaagt taaagggcgt atgaatgaac
    21181 ttatggcgct tcatacgggt caatcattag aacagattga acgtgatacc gagcgcgatc
    21241 gcttcctttc cgcccctgaa gcggtggaat acggtctggt cgattcgatt ctgacccatc
    21301 gtaattgatg ccagaggcgc aactgtgccg ctatacttat ccagggcggc acaacgctgt
    21361 aagcggcttg cgcctgagaa tggcatttgc gtcgtcgtgt gcggcacaaa gaacaaagaa
    21421 gaggttttga cccatgacag ataaacgcaa agatggctca ggcaaattgc tgtattgctc
    21481 tttttgcggc aaaagccagc atgaagtgcg caagctgatt gccggtccat ccgtgtatat
    21541 ctgcgacgaa tgtgttgatt tatgtaacga catcattcgc gaagagatta aagaagttgc
    21601 accgcatcgt gaacgcagtg cgctaccgac gccgcatgaa attcgtaacc acctggacga
    21661 ttacgttatc ggccaggaac aggcgaaaaa agtgctggcg gtcgcggtat acaaccacta
    21721 caaacgtctg cgcaacggcg ataccagcaa tggcgtcgag ttgggcaaaa gtaacattct
    21781 gctgatcggt ccgaccggtt ccggtaaaac gctgctggct gaaacgctgg cgcgcctgct
    21841 ggacgtcccg ttcaccatgg ccgacgcaac cacgctgacc gaagccggtt atgtgggcga
    21901 agacgttgaa aacatcattc agaagctgtt gcaaaagtgc gattacgacg tacagaaagc
    21961 gcagcgcggg attgtctaca tcgatgaaat cgacaagatt tctcgtaagt cagacaaccc
    22021 gtctattacc cgtgacgttt ccggtgaagg cgtacagcag gcactgttga aactgatcga
    22081 aggtacggta gctgctgttc caccgcaagg tggacgtaaa catccgcagc aggaattctt
    22141 gcaggttgat acctctaaga tcctgtttat ttgtggcggt gcgtttgccg gtctggataa
    22201 agtgatttcc catcgtgtag aaaccggctc cggcattggt tttggcgcga cggtaaaagc
    22261 gaagtccgac aaagcaagcg aaggcgagct gctggcgcag gttgaaccgg aagatctgat
    22321 caagtttggt cttatccctg agtttattgg tcgtctgccg gttgtcgcaa cgttgaatga
    22381 actgagcgaa gaagctctga ttcagatcct caaagagccg aaaaacgccc tgaccaagca
    22441 gtatcaggcg ctgtttaatc tggaaggcgt ggatctggaa ttccgtgacg aggcgctgga
    22501 tgctatcgct aagaaagcga tggcgcgtaa aaccggtgcc cgtggcctgc gttccatcgt
    22561 agaagccgca ctgctcgata ccatgtacga tctgccgtcc atggaagatg tcgaaaaagt
    22621 ggttatcgac gagtcggtaa ttgatggtca aagcaaaccg ttgctgattt atggcaagcc
    22681 ggaagcgcaa caggcatctg gtgaataatt aaccattccc atacaattag ttaaccaaaa
    22741 aggggggatt ttatctcccc tttaattttt cctctattct cggcgttgaa tgtgggggaa
    22801 acatccccat atactgacgt acatgttaat agatggcgtg aagcacagtc gtgtcatctg
    22861 attacctggc ggaaattaaa ctaagagaga gctctatgaa tcctgagcgt tctgaacgca
    22921 ttgaaatccc cgtattgccg ctgcgcgatg tggtggttta tccgcacatg gtcatcccct
    22981 tatttgtcgg gcgggaaaaa tctatccgtt gtctggaagc ggcgatggac catgataaaa
    23041 aaattatgct ggtcgcgcag aaagaagctt caacggatga gccgggtgta aacgatcttt
    23101 tcaccgtcgg gaccgtggcc tctatattgc agatgctgaa actgcctgac ggcaccgtca
    23161 aagtgctggt cgaggggtta cagcgcgcgc gtatttctgc gctctctgac aatggcgaac
    23221 acttttctgc gaaggcggag tatctggagt cgccgaccat tgatgagcga gaacaggaag
    23281 tgctggtgcg tactgcaatc agccagttcg aaggctacat caagctgaac aaaaaaatcc
    23341 caccagaagt gctgacgtcg ttgaatagca tcgacgatcc ggcgcgtctg gcggatacca
    23401 ttgctgcaca tatgccgctg aaactggctg acaaacagtc cgttctggag atgtccgacg
    23461 ttaacgaacg tctggaatat ctgatggcaa tgatggaatc ggaaatcgat ctgctgcagg
    23521 ttgagaaacg cattcgcaac cgcgttaaaa agcagatgga gaaatcccag cgtgagtact
    23581 atctgaacga gcaaatgaaa gctattcaga aagaactcgg tgagatggac gacgcgccgg
    23641 acgaaaacga agccctgaag cgcaaaatcg acgcggcgaa gatgccgaaa gaggcaaaag
    23701 agaaagcgga agcagagttg cagaagctga aaatgatgtc tccgatgtcg gcagaagcga
    23761 ccgtagtgcg tggttatatc gactggatgg tacaggtacc gtggaatgcg cgcagcaagg
    23821 tcaaaaaaga cctgcgtcag gcgcaggaaa tccttaatac cgaccattat ggtctggagc
    23881 gcgtgaaaga tcgcatcctt gagtatcttg cggttcaaag ccgtgtcaac aaaatcaagg
    23941 gaccgatcct ttgcctggta gggccgccgg gggtaggtaa aacctccctg ggtcagtcca
    24001 ttgccaaagc caccgggcgt aaatatgtcc gtatggcgct gggcggcgtg cgtgatgaag
    24061 cggaaatccg tggtcaccgc cgtacttaca tcggttctat gccgggtaaa ttgatccaga
    24121 aaatggcgaa agtgggcgtt aaaaacccgc tgttcctgct cgatgagatc gacaaaatgt
    24181 cttctgacat gcgtggcgat ccggcttccg cactgcttga agtgctggat ccagagcaga
    24241 acgtggcctt cagcgatcac tacctggaag tggattacga tctcagcgac gtgatgtttg
    24301 tcgcgacgtc gaactccatg aacattccgg caccactgct ggatcgtatg gaagtgattc
    24361 gcctctccgg ttataccgaa gatgaaaaac tgaacatcgc caaacgtcac ctgctgccga
    24421 agcagattga acgtaatgca ctgaaaaaag gtgagctgac cgtcgacgat agcgccatta
    24481 tcggcattat tcgttactac acccgtgagg cgggcgtgcg tggtctggag cgtgaaatct
    24541 ccaagctgtg ccgtaaagcg gttaagcagt tactgctcga taagtcatta aaacatatcg
    24601 aaattaacgg cgacaacctg catgactacc ttggtgttca gcgtttcgac tatggtcgcg
    24661 ctgataacga aaaccgtgtc ggtcaggtaa ccggtctggc gtggacggaa gtgggcggtg
    24721 acttgctgac cattgaaacc gcatgtgttc cgggtaaagg caaactgacc tataccggtt
    24781 cgctcggcga agtgatgcag gagtctattc aggctgcgtt aacggtggtt cgcgcgcgtg
    24841 cggaaaaact ggggatcaac cctgattttt acgaaaaacg tgacatccac gtccacgtac
    24901 cggaaggtgc gacgccgaaa gatggtccga gtgccggtat tgctatgtgc accgcgctgg
    24961 tttcttgcct gaccggtaac ccggttcgtg ccgatgtggc aatgaccggt gagatcactc
    25021 tgcgtggtca ggtactgccg atcggtggtt tgaaagaaaa actactggca gcgcatcgcg
    25081 gcgggattaa aacagtgtta attccgttcg aaaataaacg cgatctggaa gagattcctg
    25141 acaacgtaat tgccgatctg gacattcatc ctgtgaagcg cattgaggaa gttctgactc
    25201 tggcgctgca aaatgaaccg tctggcatgc aggttgtgac tgcaaaatag tgacctcgcg
    25261 caaaatgcac taataaaaac agggctggca ggctaattcg ggcttgccag cctttttttg
    25321 tctcgctaag ttagatggcg gatcgggctt gcccttatta aggggtgttg taaggggatg
    25381 gctggcctga tataactgct gcgcgttcgt accttgaagg attcaagtgc gatataaatt
    25441 ataaagagga agagaagagt gaataaatct caattgatcg acaagattgc tgcaggggct
    25501 gatatctcta aagctgcggc tggccgtgcg ttagatgcta ttattgcttc cgtaactgaa
    25561 tctctgaaag aaggggatga tgtagcactg gtaggttttg gtacttttgc cgttaaagag
    25621 cgtgctgccc gtactggccg caacccgcag accggtaaag agatcaccat cgctgccgct
    25681 aaagtaccga gcttccgtgc aggtaaagca ctgaaagacg cggtaaacta agcgttgtcc
    25741 ccagtgggga tgtgacgaag ttcaagggcg catctactga tgtgcctttt ttatttgtat
    25801 tcggtgactt tctgcgtctt gtgggctgac aattgccccc gtttcttgtc acaataggcc
    25861 tttgcgcgca tcgatacgtt gcgtgaggta cacagtcatc tacagcggag tgttgttaca
    25921 ccatgatgga cagcttacgc acggctgcaa acagtctcgt gctcaagatt attttcggta
    25981 tcattatcgt gtcgttcata ttgaccggcg tgagtggtta cctgattggc ggaggcaata
    26041 actacgccgc aaaagtgaat gaccaggaaa tcagccgtgg gcaattcgag aatgccttca
    26101 acagcgagcg taatcgcatg cagcaacagc tgggcgatca atactccgag ctggcagcga
    26161 acgaaggcta tatgaaaacc ctgcgtcaac aggtgctgaa tcgtctgatc gacgaggcgc
    26221 tgctggatca gtacgcacgt gagctgaaac tgggtatcag cgatgagcag gttaaacagg
    26281 cgattttcgc gaccccagcc ttccaggttg atggcaaatt tgataacagc cgctataacg
    26341 gtatcctcaa ccagatgggg atgaccgccg atcagtacgc ccaggcgctg cgtaaccagc
    26401 tcactaccca acagctgatt aacggcgttg ctggtaccga ttttatgctg aaaggtgaaa
    26461 ccgacgagct ggcggcactg gtcgctcaac aacgcgtggt gcgtgaagcg actatcgatg
    26521 ttaacgcgct ggcggcgaag cagcctgtga ccgaacagga aattgccagc tactacgaac
    26581 aaaacaaaaa caatttcatg acgccggaac aattccgcgt gagttacatc aagctggatg
    26641 ccgcaacgat gcagcaaccg gttagcgatg cggatctcca gagctactac gatcagcatc
    26701 aggatcaatt cacccagccg cagcgtaccc gttacagcat catccagacc aaaactgaag
    26761 atgaagcgaa agcggtactt gatgagctga ataaaggcgg tgattttgct gcattagcca
    26821 aagaaaaatc tgccgatatt atctctgctc gtaacggcgg cgatatgggt tggttagaag
    26881 atgccactat cccggatgaa ctgaaaaatg ctggtctgaa agaaaaaggc caactgtctg
    26941 gtgtcatcaa atcttcggtc ggtttcctga ttgtacgtct ggacgacatt cagccagcga
    27001 aagtgaaatc gttagacgaa gtacgtgacg atgtcgcggc gaaagtgaaa cacgaaaaag
    27061 ccctcgatgc gtactacgcg ctgcagcaga aagtgagcga tgcggcaagc aacgacaccg
    27121 agtctctggc cggtgcagag caagctgccg gcgttaaagc cactcagacg ggttggttca
    27181 gcaaagagaa cctgccggaa gagttgaact tcaagccggt tgctgacgct atctttaacg
    27241 gcggtctggt aggtgaaaac ggcgcgccgg gcatcaactc tgacatcatc accgtagacg
    27301 gcgaccgcgc attcgtgctg cgcatcagcg agcacaaacc ggaagcggtg aaaccgttgg
    27361 cagatgttca ggaacaagtt aaggcactgg ttcagcacaa caaagctgaa caacaggcga
    27421 aagtggatgc tgagaaactg ctggttgatt tgaaagccgg caaaggtgcg gaagctatgc
    27481 aggctgccgg tctgaaattt ggcgagccga aaaccttaag ccgttccggt cgtgacccga
    27541 ttagccaggc ggcgtttgca ctgccactgc cagcgaaaga caaaccgagc tacggtatgg
    27601 cgaccgatat gcaaggtaat gtggttctgc tggcgctgga tgaagtgaaa caaggttcaa
    27661 tgccggaaga tcagaaaaaa gcgatggtgc agggtatcac ccagaacaac gcacaaatcg
    27721 tctttgaagc tctgatgagt aacctgcgta aagaggcgaa aatcaaaatt ggcgatgcgt
    27781 tggaacagca ataatcctga agccgcctcg caaaaaaatg ctttgcagct gtaacaagaa
    27841 aaggtcgctt tcgcggcctt ttccatttct gaacattgcc atttgtttac tgttttcact
    27901 gccgttaagg tgattccact gttaacaaac aaggagaaaa cagtatgaaa cacggaatta
    27961 aagcactgct cattaccctg tccctggcct gtgccggaat gtctcatagc gcgctggcgg
    28021 cagcttctgt ggcgaaaccg acggcggtag aaaccaaagc ggaagctcct gcagcacaaa
    28081 ataaagcagc agtaccggcg aaagccagtg acgaagaagg ctcccgggtc agcattaata
    28141 atgccagcgc ggaagagcta gcccgcgcga tgaatggcgt tggcctgaag aaagcgcaag
    28201 cgattgtcag ttatcgcgaa gagtacggtc cgtttaaaac ggtggaggat ctaaagcagg
    28261 tgccggggat gggcaattcg ctggtggaac gtaatctggc ggtattaacc ctgtaattaa
    28321 tttgcatagt ggcaattttt tgccagactg aagaggtcat accagttatg acctctgtac
    28381 ttataacagt ttttataaaa acaacgtaag gttattgcgc tatgcaaaca caaatcaaag
    28441 ttcgtggata tcatctcgac gtttaccagc acgtaaacaa cgcccgctac cttgaatttc
    28501 tcgaagaagc ccgctgggat gggttggaaa atagcgacag ttttcactgg atgacggccc
    28561 ataacatcgc tttcgtggtg gtgaatatca atattaacta tcgtcgtccg gcggtattaa
    28621 gcgacttgtt aaccattacg agccagttac agcaattaaa cggtaaaagc ggcatcttaa
    28681 gccaggtcat tacactggag ccggaagggc aggtggtagc ggatgcgctt attacgtttg
    28741 tttgtattga tcttaaaacg cagaaagcat tagctctgga aggggaattg cgcgaaaagc
    28801 tggagcagat ggttaagtaa acgttttgtg gtgccggatg ctcaagccgc atccggcgac
    28861 acccggaata attacttcaa cccggttttc tgcttcatcg ctgccatcac cgtcggttta
    28921 tcggccagat aatgattcaa accgttggcg cgtaaattac atgccgcaca atgaccgcaa
    28981 ccgtcgcctt taatgccgtt atagcaggtc aacgtttcgt tacggactaa atccagtttg
    29041 ccgtaataat ctgccagcgc ccaggtttcc gctttatcaa tccacatcag cggcgtttca
    29101 aaacgaatat ctttcgccat gcccaaactg acggcatggt ttagtacttt cacaaactca
    29161 tcgcggcaat ccgggtagcc ggagaaatcc gtttcgcaga cgccggtaat tacggcttct
    29221 gcttttacct gatacgcata tattgccgcc agcgtcagga acaaaatatt acgccctggg
    29281 acaaacgtat tcgggatgcc atcggcttca ggttcataat caggcaccgg aatgctgtca
    29341 cgcgtcaggc tgctgaccgc cagctcgttg agcagcgtta catcaagaac cttatgcgcg
    29401 cgtgccccca gtttcagcgc cagttcgcgt gccacgtcga tttctgcgcg atggcgctga
    29461 ccgtaatcga acgtcacgca atggacttca tcatattgtt gtaatgcctg caccagacag
    29521 gtggtggaat cctggcctcc actgaacaca acgacagcac gtttcataga taatcctgct
    29581 tgaacaataa aagcgttatg gtaacgcctg cgattaaccc ggaccagcta ttcattgcga
    29641 cggcgcggga agccaggctt cggtaaattc aaaccagccg cgcggtgtca gtcgcacacc
    29701 gttcacgccg ggaggggcac taatgcgata gtgatagttg aacagcggcg tcagcgtcgc
    29761 atctgtcatt aactggctaa aaaccgcttt cagggcatta aatcggtttt cctcatcagg
    29821 cattacttgc accgcgtcca gtgtcgattg tagatgtgcg tatgctggag cgtcgaaaac
    29881 atgtggccac agcggatcgc agcgcagcca ttgctccaga gtatattccg gtgcttcgcc
    29941 aattaatctg tcgcccatca tgaggtctgc gtgtgccagt agggtcgtgt cgtcccagtt
    30001 ttttgcgtta tgaaaaataa ttgtgagttc acagccttcc gctgccagtg tcgcctgtag
    30061 gcgttctgcc atggtatgaa gttctatcgg gaggtgataa accagcgtca aggttttcgg
    30121 tagtttgact tcatccggta cttgccaatg cggaatagtc cagcctggca gtaatgcatg
    30181 actggcggtg atcaggtttt ctccgacttc taacgtttgt aataaaccgg attgatgaat
    30241 aatggagatc acttttcgcg cctgccagag ggagagtcgg ggacttttgc gcaacgttaa
    30301 atagcaaaaa cctaaactga tgccactact gacctggctg acccgttgca gctcctccgg
    30361 tttgccgatg gtgatttgca cgggatgccg acaactggtt cccaaatctt tttcgaaaag
    30421 cggcggagtt atccagtact caaccgcttt aagcagcgga tgacgtaaat ggtaataatc
    30481 atggctttcc aggcgcacca gttcagctgt gaattgtgtt aagcgaaaag gacccgtgcc
    30541 aatcagtggg aattgcggat gcgccagatg gctgcaatag ctcgccagcc ggtgcgcaag
    30601 ccagtaatcg gggcgatgta aaaagaaggt cagacactgc ggatgggtga cctcaatacg
    30661 cttcacgcta ataaataatt gatccagtgc tggcagttgt aacagcatca ataatcgctg
    30721 gtgtaagtgt gaggctttta ctgcatcgcc gttatgccag tgtagggttg aacgaagata
    30781 aaagtcccag cgtaacccgt cagcagaggt ttcccagtga tgcgctaaat cgccaatcgg
    30841 gcgctgggta ttattatcga agcgggtcag gccggaaaat atctgtccgg cgagatgctg
    30901 ctcggcacgg ccgggcaaaa agcccggttg tagcggttcg agcgggcgat agtagggaat
    30961 acgcaacgtg ggtgtatcgt tttgccattg tccgcccata aacggctgta acagagtgcg
    31021 cagctcacct ggggccagtt gcgccagctc cagcacatct tgctgctttc cggtttccag
    31081 tgccttttcc atcatcgcat tgcgtagcga ttctggcgtg accagaaagc gtaattgtcc
    31141 gcgctttccg cgtcctgact gcgcctgcca ctccagccat cccgcttcct gtgcctgacg
    31201 caacagcgta cgaacatggc gttcgctgca aaaacagcgt tcggccagtt cgctgacggt
    31261 gacggtttgc ggctttccgg cggaaggttg ccacagacgt tgatactggt taaggcggtt
    31321 gagcagtcgc ataaaaaccc ggaacaatat tatttaacta ttcactatta cttccgtata
    31381 tatcaggtga tactcaatca ccattaaccg tgtcacagag tggagaaaga aatggctcgt
    31441 ctggcagcat ttgatatgga tggcacttta ttgatgcctg accatcattt aggtgagaaa
    31501 accctctcta ctttggcgcg actgcgtgaa cgcgacatta ccctcacttt tgccacgggg
    31561 cgtcatgcgc tggagatgca gcatattctc ggggcgatat cgctggatgc gtatttgatt
    31621 accggcaacg gaacgcgcgt gcattctctg gaaggtgaac ttttacatcg tgatgattta
    31681 cctgcggatg tcgcggagct ggtgctgtat cagcaatggg atacccgagc cagcatgcat
    31741 attttcaatg acgacggttg gtttaccggg aaagagatcc ctgcgttgtt gcaggcattt
    31801 gtctatagcg gttttcgtta tcaaataatc gatgtcaaaa aaatgccact cggcagcgtc
    31861 accaagatct gcttctgtgg cgatcacgac gatcttacac gtttgcagat ccagctacac
    31921 gaagcattag gcgagcgtgc acatttgtgt ttttccgcca cggattgcct tgaagtgctg
    31981 ccggtgggct gcaataaagg cgctgcactg acggtgctga cccaacattt aggtttatcg
    32041 ttgcgcgatt gcatggcctt tggtgatgcg atgaacgatc gcgaaatgtt aggcagcgtc
    32101 ggtagcggat ttattatggg caatgcgatg ccgcaactgc gcgcggagct cccgcattta
    32161 ccggtgattg gacattgccg aaatcaggct gtctctcact atttgacgca ctggctggac
    32221 tatccacatc taccttattc ccccgaataa cgagatccct tccagcaccg ggcaattgcc
    32281 cggttttttt gcgttgaatt tgtcattttg tgccgtggtg tttaaaccgc acagaataaa
    32341 ttgtcgcgat ttcaccttta aaatagaatt aaaagagaaa aaaattctcc gtggaagggc
    32401 tatgttagat aaaattgacc gtaagctgct ggccttacta cagcaggatt gcaccctctc
    32461 tttgcaggca ctggctgaag ccgttaatct gacaaccacc ccctgctgga agcgcctgaa
    32521 acggctggag gacgacggta tccttatcgg caaagtcgcc ctgctggatc cggaaaaaat
    32581 aggcctcggc ctgaccgcct ttgtgctgat aaaaacgcaa catcacagca gcgaatggta
    32641 ttgccgcttt gtcacggtgg ttaccgaaat gccagaagtg ctggggttct ggcgcatggc
    32701 tggtgaatac gattatctga tgcgcgtcca ggttgccgac atgaaacgct acgacgagtt
    32761 ttataagcgt ctggtaaaca gcgtgccggg gctgtcggac gtcacttcca gcttcgcgat
    32821 ggaacagatt aaatacacca cttctttacc catcgaataa atatccagaa tcaggtcagg
    32881 acacaacgcg tgcgattatt tgctcaatta agctggtatt tccgtcggga atggcgtcgc
    32941 tatctcgggg ctgtcgcctt gcttgtcatt atcgcgatgc tgcaactggt tccaccaaaa
    33001 gtggttggta ttgttgtcga tggcgtgaca gaacaacact ttactaccgg gcagatcctg
    33061 atgtggatcg ccaccatggt gctgattgcc gttgtggttt atctcctgcg ttacgtctgg
    33121 cgcgtattgc tgtttggtgc gtcttatcaa ctggctgttg aactgcgtga agattattac
    33181 cgtcagctaa gccggcagca tcctgagttt tacctgcgtc atcgcacggg tgacctcatg
    33241 gctcgtgcga ctaatgacgt cgatcgcgtc gtgtttgccg ccggagaagg ggtgctgacg
    33301 ctggtggatt cactggtaat gggctgcgct gtgttgatta tgatgtctac gcaaattagc
    33361 tggcagttga ccttatttgc cctgttaccg atgccagtga tggcgatcat gattaagcgc
    33421 aacggcgatg ctttgcatga acgctttaag ctggcacagg cggcgttttc cagtcttaat
    33481 gaccgcaccc aggaaagcct caccagtatc cgcatgatca aagcctttgg tctggaagat
    33541 cgccagtcgg cgttatttgc cgcggatgcc gaagataccg gcaaaaaaaa catgcgggtg
    33601 gcgcgtattg atgctcgttt cgacccgacc atctatatcg cgattggtat ggcgaacttg
    33661 ctggcgattg gcggcggtag ttggatggtg gtgcagggca gtttaacgct gggccagctc
    33721 accagtttta tgatgtattt aggtctgatg atttggccaa tgctggcgct ggcatggatg
    33781 tttaacattg tggaacgtgg tagtgctgcg tacagccgta ttcgcgcgat gctggcggaa
    33841 gcgccggtgg tgaacgatgg tagcgaaccc gtgccggaag ggcgtggcga actggatgta
    33901 aatattcacc agttcacgta tccgcagact gaccatcctg cgctggaaaa cgtcaatttc
    33961 gccctgaaac ccggtcagat gctgggtatc tgcgggccga ctggttccgg caaaagtacc
    34021 ctgttgtcgc tcattcagcg tcatttcgac gtcagcgagg gggatattcg ctttcatgat
    34081 attcctctga cgaagttaca actcgatagc tggcgtagcc gcctggcggt agttagccag
    34141 acgccattcc ttttttctga caccgtggcg aataacatcg cgctgggttg cccgaatgcc
    34201 acccagcaag agattgagca tgtcgcgcgg ttagccagcg tacatgacga tattttgcgt
    34261 ctaccgcaag gttacgatac agaggtgggc gagcgcggtg tgatgctttc cggcgggcaa
    34321 aaacagcgta tctccattgc tcgtgcgtta ttagtcaacg cggaaatcct catccttgat
    34381 gatgcgcttt cggcggtgga cggacgcact gagcaccaga tcctgcataa cctgcgtcag
    34441 tgggggcagg gaagaacggt aatcatcagt gcccatcgcc tttctgcact gacggaagcc
    34501 agtgaaatta ttgtgatgca gcacggacat atcgcccagc gtggcaatca tgatgtgctg
    34561 gcacaacaaa gcggctggta tcgcgatatg tatcgctatc aacaactgga ggcggcgctc
    34621 gacgacgctc cggaaaatcg cgaggaggcc gtcgatgcgt agttttagcc aactgtggcc
    34681 gactctcaag cgcctgttag cgtacggttc accgtggcgt aaaccgctgg ggattgcggt
    34741 cctgatgatg tgggttgcgg cggcggcaga agtcagtggg ccgctgctta tcagctattt
    34801 tatcgacaat atggtagcga aaaataacct gccgttgaaa gtggttgcag ggctggctgc
    34861 ggcgtatgtt gggctgcaac tgtttgccgc cgggctacat tacgcgcagt cgctgctgtt
    34921 taatcgggcg gcagtaggcg tagtgcaaca gttgcgtacc gacgtgatgg atgctgcgtt
    34981 acgccagcca ttaagcgagt ttgataccca acccgtcggg caggtgattt cccgcgtcac
    35041 taatgacact gaagtgatcc gcgatctcta cgttaccgta gtggcaactg tcctgcgcag
    35101 tgccgcactg gtgggcgcga tgctggtggc gatgttcagc ctcgactggc gaatggcgct
    35161 ggtggcgata atgattttcc cggtggtgct ggtggtaatg gtgatatacc agcgttacag
    35221 cacgccaatt gtccgtcgtg tgcgcgccta tctggcggat atcaacgacg gctttaacga
    35281 aatcatcaat ggcatgagcg ttatccagca gtttcgtcag caggcgcgat ttggcgaacg
    35341 tatgggtgag gccagtcgtt cacactatat ggcgaggatg caaaccctgc gcctcgacgg
    35401 ttttctgctg cgtccgctgc tgagtctgtt ttcatcgctc attctctgtg gcttgttgat
    35461 gctgtttggc ttctccgcca gcggcaccat tgaagtgggc gtgttgtatg cgtttatcag
    35521 ctatcttggg cgacttaacg aacccttaat cgaactgacc acgcagcagg cgatgctgca
    35581 acaggctgtt gttgctggtg agcgcgtgtt tgagctgatg gacggaccgc gccagcaata
    35641 tggcaatgat gatcgcccgt tacagagtgg caccatcgaa gtcgataacg tgtcatttgc
    35701 ttatcgcgat gacaatctgg tgctaaagaa cattaatctc tctgtgcctt cgcgtaactt
    35761 tgtggcgctg gtcgggcata ccggcagtgg taaaagtacc ctcgccagtt tattgatggg
    35821 ctattacccg ctaacggaag gtgagattcg ccttgatggt cgtccattaa gttcgctaag
    35881 tcacagcgcg ctgcgccagg gcgtggcaat ggtgcagcaa gatccggtgg tgctggcgga
    35941 taccttcctc gccaacgtga cgctggggcg ggatatctcc gaagaacgcg tctggcaggc
    36001 gctggaaacc gtgcaactgg cggagctggc acgcagtatg agcgacggga tttatacgcc
    36061 gctgggcgag caggggaata atctctctgt cgggcaaaag caactgctgg cactggcgcg
    36121 cgtgctggtc gagacgccgc aaatcctgat ccttgatgag gcaaccgcca gcattgactc
    36181 cggtactgaa caggcgattc aacatgctct ggcggcggtg cgtgaacata ccacgctggt
    36241 agtgattgct caccgcttat cgactattgt tgatgccgac actattctgg tgcttcatcg
    36301 tgggcaagcc gtggagcagg gcactcacca gcaactgctg gcggcccagg gacgctactg
    36361 gcagatgtat caactgcaac ttgcgggcga agagcttgca gccagcgtgc gtgaagaaga
    36421 atcattgagc gcctgaatag cgcaatatct catcgttggt gcaaaaatgt aacgcactgt
    36481 gcactgtcat agtgcgtttt cattttcaat cttcttaact tcctgctctc tttctcgttt
    36541 ttcatttctg gcacaccgct tgcaatacct tcttcgtgta gcagaaccat taccgaattc
    36601 tgaccggagg ggatctatga agctggtgac cgtgataatc aaaccattca agctggaaga
    36661 cgttcgtgaa gcgttatctt ccattggtat tcagggcctg accgtcaccg aagtgaaagg
    36721 tttcgggcgt cagaaagggc atgccgagct gtaccggggg gcggaataca gcgtcaactt
    36781 cctgccaaaa gtaaaaattg atgtggcgat tgctgatgac caactcgatg aagtgatcga
    36841 tatcgtcagt aaggcggctt acaccggaaa aattggcgac ggcaaaatct tcgtcgctga
    36901 attgcaacgc gtcattcgta ttcgtaccgg cgaagccgac gaagcggcgc tgtaatctct
    36961 ggcacacagc aacaggaacg aaaaatgaag atagcgacga taaaaactgg gcttgcttca
    37021 ctggcgatgc tgccgggact ggtaatggct gcacctgcgg tggccgataa agccgacaat
    37081 gcgtttatga tgatttgtac tgcactggtg ctgtttatga ctattccggg gattgccctg
    37141 ttttacggtg ggttgattcg cggcaaaaac gtgctgtcga tgctgacgca ggtgacggtg
    37201 acgtttgcac tggtctgtat tctctgggtg gtttacggtt actcgctggc gtttggtgag
    37261 ggcaacaact tcttcggcaa cattaactgg ttgatgctga aaaacatcga actgacggcg
    37321 gtgatgggca gcatttatca gtatatccac gtagcgtttc agggatcgtt tgcctgcatt
    37381 accgtaggct tgatagttgg ggcgctggcg gaacgaatcc gcttctcagc tgtgttgatt
    37441 ttcgtggtgg tatggctgac gctctcttac attcctattg cgcatatggt gtggggcggt
    37501 ggtttgctgg cttctcacgg tgcgctggat ttcgcgggtg gcacggtggt gcatattaac
    37561 gccgcaatcg ccggtctggt gggcgcgtat ctgataggca aacgcgtggg cttcggtaaa
    37621 gaggcgttta aaccgcacaa cctgccgatg gtcttcaccg ggactgccat tctctatatc
    37681 ggttggtttg gctttaacgc cgggtcagcg ggcacggcga atgaaatcgc ggcactggca
    37741 tttgtgaata ctgtggtcgc aacggcggcg gcaattcttg gctggatctt cggtgaatgg
    37801 gcgctgcgtg gtaagccttc actgctggga gcgtgttctg gcgcgattgc cggtctggtc
    37861 ggcgtgacgc cagcgtgtgg ctacattggt gttggcggcg cgttgattat cggcgtggta
    37921 gctggtctgg cgggcttgtg gggcgttacc atgctcaaac gcttgctgcg ggtggatgat
    37981 ccctgcgatg tcttcggtgt gcacggcgtt tgtggcattg tcggctgtat catgactggg
    38041 atttttgccg ccagctcgct gggcggcgtg ggcttcgctg aaggtgtgac gatgggccat
    38101 cagttgctgg tacagttgga aagcattgcc attacgattg tgtggtctgg tgtggtggca
    38161 tttatcggct acaaactggc ggatctgacg gttggtctgc gtgtgccgga agaacaagag
    38221 cgagaagggc tggacgtcaa cagtcacggc gagaatgcct ataacgcata acaagcactg
    38281 caaaaaacag ccggacggtt ttcacctccg gctatttttt taattgtgat tacgcatcac
    38341 cccttcctga acggtcgagg caaccagtac gccgtcctgg gtataaaact caccgcgcac
    38401 aaagccacgt gcgctggacg ccgaggtgct ctccacgcta tacagcagcc attcattcaa
    38461 attaaacggg cgatggaacc acatggaatg gtcaatggtg gcaatctgaa tccccggttc
    38521 gagaaaaccg ataccgtgcg gctgtagagc taccggcagg aagttaagat cagaagcgta
    38581 accgagcaga tactgatgaa cgcgcaggtc atccggcacg ctaccatttg cgcggatcca
    38641 cacctgacga tgtggttctg cgacgtgacc tttcagtggg ttatgaaact ccaccggacg
    38701 gacttccagc ggacgatcgc agatgaattt atctttaagc actggcggca gcaggtgcgc
    38761 cagcgattgg gcgatttgcg tttccgaagg caggccatca ggcgctggcg cggacggcat
    38821 tgttttttga tgttcgaaac ccgcttctgg tgcctggaaa gaggccgtca tataaaaaat
    38881 cggtttgccg ttttgaatag cagcaacccg gcgggcgctg aagctgttac cgtcacgcag
    38941 cgtttcgaca tcataaataa tcggcttctt actatcgcca gggcgaagaa agtagctgtg
    39001 aaacgaatgt accagccgct cttcagggac ggtctctttt gcagcataca aggcctgacc
    39061 cacgacctgg ccgccaaata cctggcgtaa acctaaatct tcactctggc cgcgaaagag
    39121 tccttcctca attttttcca gatttaacaa tgtcagtaaa ttttttagcg cctgactcat
    39181 ataactctcc agtaacaaag ctgccgcagc aagccaaagt gagttgagta taacgcaaat
    39241 ttgctactgg tccgatgggt gcaatggtct gaattacggg ctaattacag gcagaaatgc
    39301 gtgatgtgtg ccacaattgt tgacgttact attttgttaa ccactcttcc ggcgaggaaa
    39361 gttagcccgc tggtgcattg ataataagga gaaatgaatg aaactcgtgc acatggccag
    39421 tggtttagcg gttgcgattg cgttggcggc ttgcgcagat aaaagcgcgg atattcagac
    39481 gccagccccg gctgcaaata cgtctatttc agcaacacaa caacctgcta tccagcaacc
    39541 gaatgtctcc ggtaccgtct ggatccgtca gaaagtcgca ctgccgcctg atgctgtgct
    39601 gaccgtgaca ctttctgacg cgtcgttagc cgatgcaccg tcaaaagtgc tggcgcagaa
    39661 agcggtgcgt actgaaggta aacagtcacc attcagcttt gttctgccat ttaacccggc
    39721 agatgttcag ccgaacgcgc gtattctgtt gagtgcggcg attaccgtga atgacaaact
    39781 ggtatttatt accgataccg ttcagccggt gatcaaccag gggggaacca aagccgacct
    39841 gacattggtt ccggttcagc aaaccgccgt gcctgtccag gccagtggtg gcgcaacaac
    39901 taccgtacca tcgacttcac cgacccaggt gaatccatct tcggcagttc ctgcccctac
    39961 gcaatactaa gcgttactaa cccctttcca tctggagagg ggttagtagt tccagcgata
    40021 gcgctgtaag tcgatttgcc cgcttcccga taccatcaca ccttctgcca gtaatgcctg
    40081 tcgttgacgc tgtaaatccg gtccggttag cgaaatggtg ccgtggcgat taaccacccg
    40141 gtgccagggc aaggtgctgc cttcggggag acgctttaac acaccgccaa cctggcgcgc
    40201 ggcgcggggc gatcccgcca gtttcgccac atcaccgtaa gtggtgacat acccttcggg
    40261 aatagcggct acgatttgcc agacgcgttg gggaaatgaa tcttcttttt ccatcttttc
    40321 ttcctgagat aatttttcag cataatctgg aaaaacgccc gagtgaagcc gcattgcgca
    40381 agaaaccagc atctggcacg caatgggttg caattagccg gggcagcagt gataatgcgc
    40441 ctgcgcgttg gttctcaacg ctctcaatgg gggctctgtt ggttctcccg caacgctact
    40501 ctgtttacca ggtcaggtcc ggaaggaagc agccaaggca gatgacgcgt gtgccgggat
    40561 gtagctggca gggcccccac ccatttctgc ctcccaccgt ttcgtcaaaa aatcccaaca
    40621 tgactaaact ttaaccacaa ctgacgtcgc aagaattgtc tggctgcgca gtacgcttcg
    40681 gaggtatgtc tgatgaagta tgttgatggt tttgtggttg ccgttcctgc cgataaaaag
    40741 gatgcctatc gggaaatggc cgctaaggcc gcgccattgt ttaaagagtt tggcgcgctt
    40801 cgtattgtcg aatgctgggc cagcgatgta ccggatggca aagtgaccga ttttcgtatg
    40861 gcggtgaaag cggaagagaa tgaagaggtt gtctttagct ggattgaata cccttcaaaa
    40921 gaggtccgcg acgcagctaa tcaaaagatg atgtcggacc cacggatgaa agagttcggc
    40981 gagtccatgc cgtttgacgg caagcgaatg atctatggcg gattcgagtc aatcatcgac
    41041 gaataggtag cgtgacgggc tgtgcggcgt gccgtcagcc caaaaattca caaatgttgc
    41101 tcggcccagc gtaagaaatc ttctttcggt aatgccttgc tgtagagcca gccctgaccg
    41161 tagtgcacgc catgctggcg tagccactct tcttgtttac tggtttcgat tccctccgct
    41221 accattttca gtttcagtgt ttttgccatt tcgatgatat gcggcgtgac atttttatat
    41281 tccagcgcat caacgaaaga tttatcgatc ttcagaatgt cgacatccag atcctgtaaa
    41341 taacttaaac ttgaataccc cgtaccaaaa tcatcaagat aaatttcatg gcccgcctcc
    41401 cggtagcgag aaattatcgg ggcgctggtt ttcggatcgg caaactcgcg ttcagtgagt
    41461 tcaagcgcga tctgtctggg attaacctga tagtgattga tcatttcacg cagcaattgc
    41521 gggatttttt ccgaggtgag cacggtggat tcaagattga tcgaaatatg ctgctgtgga
    41581 tgctgacgca gccagtcgcc catatcttca aagacgcttc ttataatcag tagcgtcaat
    41641 ggctcagaaa ggcccgtttg ctgtgccagc ggaataaaac tatctggtga caaccaacta
    41701 ccgtctgtct gcggccagcg cgccagtgcc tcagcaccga caattttgcc attggctaag
    41761 gagacaatcg gctgatagtg cacgcaaata tcacgatttt cgatagcatc ctgcagccga
    41821 tgatgcggtg actggatgcg gcgcaaaatg cgcagcacaa acatcgctgc cagcaggcca
    41881 atcaccaacc cgagcggtaa ccaaataaag acttgccgat gccagccttt ctgcaacatt
    41941 ttcgatgaag cccaggtgat aattgaaata ttcatctccg ggaagggcag gatatcgtaa
    42001 atgattccat tattttcgat atgctcaccg ggtgtttttt gtagcctggt aataattccc
    42061 tgagcaattt catcgctgct ggttatgaca acgttatggg cattgccaat aatggcggca
    42121 tcaatttgcc atgagctata gggaatgaca tcaataaagg aagcggggtc gatcatgacg
    42181 acataatgtg ccgttcccat ggcgaccatg taacggataa tgcctaaatc gttatgtgat
    42241 gttaaccaga cacgataacc atctttcgaa attttacctg gctcggggaa ggtatcgggc
    42301 ggactttcat gctccagaga cgaacactgg ggaacgttgt tatcgatata aaccacttcc
    42361 tgaatatagc gataactgta agatacccga cgcatttcca tgagatgggc ttcactacag
    42421 tcagcgcctt gccatctttc cagctcttgc agcgcatctt tcccttgtgt cgccacctta
    42481 ttggcccgaa tagcgacgcg ggaggaatag gtatccagct cttcaataaa cgatgtttct
    42541 acctgctgat gggccagcca gatgcttaag ccgacaggca gcaatactga aagaatcagt
    42601 actcccgaaa taaggccgac cagatgtcgt gttctcacca gcatatcctt attaacacag
    42661 tgtagggaaa gtatatctga ttgtggaaaa aggctggcag gagataagga ggtagagctg
    42721 gtatttcatg aagtgatggg tggaaacgat cgttgtcatt tgaccaggcg cagagcttta
    42781 cgcctggtat gtaatcctaa cttaagatgt tgattcggcg cgagcgaggg taaaaatatc
    42841 gtaaaaagac aattcacctt tacggacgat cattttttgc agttgttcct tatgaacatc
    42901 cgcgacacgc ggagagttca agatggcttc aaccagcgcg gcaggtacaa agcgcgtgtt
    42961 gtaccattcg ccgttatagc aaacgcggaa atccaacacg tcgatatctt catagcggta
    43021 gcgtgggtag acatcgaaaa agaagaaacc attaagcagg ataaataata caaccagtag
    43081 ccagacagag cctgaaagcg tttcggactg caacatcacc gccagggtgg caaaaaaagc
    43141 aacgtacata ccgataaaca gccccggatg tttacggata aaactgatac taaagcgggg
    43201 tttgttgtcg cgtttttcgc gggtattcag agactcaatc gtttcggtca gcaggcgttg
    43261 tatttcggtc atcgtttgcc ttcgtggtat ctgcaacttt ccagggaaca ccctatttta
    43321 acgtgggaat agtagaggga aagtaacaga tctgccacaa ttttgcataa cagttgcgaa
    43381 aaaccgatta caatttttta attattctgg ctggattacc gcccacgaca acgttgtccg
    43441 ggacatcttt tgtgacaact gcacctgagg ctaccacgac gttatcacca atggtcacac
    43501 cagggttaat gaccgcgcgt ccgccaatcc agacgttatt accgatggtg acgggtttcc
    43561 ccagttcagc accgctatta cgtgctacag ggtcgatggg atgtgttgcc gtgtagatat
    43621 gaacgcctag tgccaacata cagttatcac cgatgcgaat agggcagaca tcaagcatca
    43681 cgcaatcgaa gttggcgaaa aaattattac cgagaaaaat gttatagcca tagtcacagc
    43741 gaaacgttgg ctcaatataa gcctctgtca cctgaccgaa tagatcagcg agaatttgct
    43801 ggcgtaatgt gtgctcttcc gccagggaat gattgtatcg gtgaataagc tgacgagcgc
    43861 gcaggcgatc gcgagataac gtctcatctg ccgagcgata caactcacca gcaatcatct
    43921 tttctttttc tgtgctcatt actgacctcc ggttgctgaa aatgcggcaa cacagtaaga
    43981 cgaaaagtca gtgaagagaa cgttcccatt acatctgtgt gataaagatc acatagggta
    44041 tgtcttcatg gcgaaaaagt ataaaattct taataaacag ccggttatag ctccgaaagc
    44101 gaattattta ttagcgaata aatttccata ctgaggaagg gatcttgtcg tacagtttat
    44161 tcatggtcaa ttcggcgagg cggtgatctg cggctgagta aaataccgcc agttcattat
    44221 ctgataattc gtatttattt ttctcgataa cacgctccag cgtgtcaatt gtctggcaac
    44281 gacgtaaacg cattaaataa tcggttttcg ttaaaggttt ttcggacata cttctaccca
    44341 tggttgtaat aattctaaca agataaactc gcaggattct ctttcgtcgc attgacaaaa
    44401 caacggaata gtcgattacc tgacttccgc catttctgaa gatcctgcat attaatacca
    44461 taactactga acaacataaa ggtgtcatcc agatattcgt cgatctgctc aatgagctta
    44521 ttgtcttcat tatacttaat tttgtaatta agtgcgaagg tcgcaatatg ctcaatcagt
    44581 tcattcaact ggaggttgat cgccgaggtt gggtcgttaa cccagccatg attgctttct
    44641 tcaaggtttg caaggcagtc atgatacaga gtttcacaga gaaacttaag ctgtgcgata
    44701 tcatgtcttt tgggtgagta ttcatccata acgcgtcccc ttcttagcgg ttgaactaac
    44761 ggacaccttt cgggatggaa aaaacttact gacctggact tgccctctcg tttgttagct
    44821 taactataag ccactctttg caggttttca tcgcattatt acgaaaaatt acaattagga
    44881 aggaagcggg acagggctca ctttttcata ctagcaccaa cccgcagcag gtaaaagcag
    44941 tttgctcgaa ctgttcataa catttcagga ttatcgccag atgaaattgg tgacactaat
    45001 accaggattg ctctgaat
//
LOCUS       DAZKIF010000021        43419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZKIF010000021 DAZKIF010000000
VERSION     DAZKIF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.021"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            171..1739
                     /gene="cydA"
                     /locus_tag="ACHOTW_001210"
     CDS             171..1739
                     /gene="cydA"
                     /locus_tag="ACHOTW_001210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP9843362.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1755..2894
                     /gene="cydB"
                     /locus_tag="ACHOTW_001211"
     CDS             1755..2894
                     /gene="cydB"
                     /locus_tag="ACHOTW_001211"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9843363.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2909..3022
                     /gene="cydX"
                     /locus_tag="ACHOTW_001212"
     CDS             2909..3022
                     /gene="cydX"
                     /locus_tag="ACHOTW_001212"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP9843364.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3022..3315
                     /gene="ybgE"
                     /locus_tag="ACHOTW_001213"
     CDS             3022..3315
                     /gene="ybgE"
                     /locus_tag="ACHOTW_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP9843365.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3465..3869
                     /gene="ybgC"
                     /locus_tag="ACHOTW_001214"
     CDS             3465..3869
                     /gene="ybgC"
                     /locus_tag="ACHOTW_001214"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9843366.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3866..4558
                     /gene="tolQ"
                     /locus_tag="ACHOTW_001215"
     CDS             3866..4558
                     /gene="tolQ"
                     /locus_tag="ACHOTW_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP9843367.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4562..4990
                     /gene="tolR"
                     /locus_tag="ACHOTW_001216"
     CDS             4562..4990
                     /gene="tolR"
                     /locus_tag="ACHOTW_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP9843368.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5055..6239
                     /gene="tolA"
                     /locus_tag="ACHOTW_001217"
     CDS             5055..6239
                     /gene="tolA"
                     /locus_tag="ACHOTW_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP9843369.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSS
                     GKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGK
                     TCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDF
                     KP"
     gene            6372..7664
                     /gene="tolB"
                     /locus_tag="ACHOTW_001218"
     CDS             6372..7664
                     /gene="tolB"
                     /locus_tag="ACHOTW_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP9843370.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7699..8220
                     /gene="pal"
                     /locus_tag="ACHOTW_001219"
     CDS             7699..8220
                     /gene="pal"
                     /locus_tag="ACHOTW_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP9843371.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8230..9021
                     /gene="cpoB"
                     /locus_tag="ACHOTW_001220"
     CDS             8230..9021
                     /gene="cpoB"
                     /locus_tag="ACHOTW_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP9843372.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9186..9261
                     /locus_tag="ACHOTW_001221"
     tRNA            9186..9261
                     /locus_tag="ACHOTW_001221"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9219..9221,aa:Lys,seq:ttt)
     gene            9297..9372
                     /locus_tag="ACHOTW_001222"
     tRNA            9297..9372
                     /locus_tag="ACHOTW_001222"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9330..9332,aa:Val,seq:tac)
     gene            9375..9450
                     /locus_tag="ACHOTW_001223"
     tRNA            9375..9450
                     /locus_tag="ACHOTW_001223"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9408..9410,aa:Lys,seq:ttt)
     gene            9502..9577
                     /locus_tag="ACHOTW_001224"
     tRNA            9502..9577
                     /locus_tag="ACHOTW_001224"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9535..9537,aa:Val,seq:tac)
     gene            9581..9656
                     /locus_tag="ACHOTW_001225"
     tRNA            9581..9656
                     /locus_tag="ACHOTW_001225"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9614..9616,aa:Lys,seq:ttt)
     gene            9803..9878
                     /locus_tag="ACHOTW_001226"
     tRNA            9803..9878
                     /locus_tag="ACHOTW_001226"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9836..9838,aa:Lys,seq:ttt)
     gene            10156..11199
                     /gene="nadA"
                     /locus_tag="ACHOTW_001227"
     CDS             10156..11199
                     /gene="nadA"
                     /locus_tag="ACHOTW_001227"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP9843373.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11237..11956
                     /gene="pnuC"
                     /locus_tag="ACHOTW_001228"
     CDS             11237..11956
                     /gene="pnuC"
                     /locus_tag="ACHOTW_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9843374.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11953..12894)
                     /gene="zitB"
                     /locus_tag="ACHOTW_001229"
     CDS             complement(11953..12894)
                     /gene="zitB"
                     /locus_tag="ACHOTW_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP9843375.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13008..13388)
                     /gene="ybgS"
                     /locus_tag="ACHOTW_001230"
     CDS             complement(13008..13388)
                     /gene="ybgS"
                     /locus_tag="ACHOTW_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP9843376.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13704..14756
                     /gene="aroG"
                     /locus_tag="ACHOTW_001231"
     CDS             13704..14756
                     /gene="aroG"
                     /locus_tag="ACHOTW_001231"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP9843377.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(14922..15674)
                     /gene="gpmA"
                     /locus_tag="ACHOTW_001232"
     CDS             complement(14922..15674)
                     /gene="gpmA"
                     /locus_tag="ACHOTW_001232"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9843378.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(15876..16916)
                     /gene="galM"
                     /locus_tag="ACHOTW_001233"
     CDS             complement(15876..16916)
                     /gene="galM"
                     /locus_tag="ACHOTW_001233"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP9843379.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            complement(16910..18058)
                     /gene="galK"
                     /locus_tag="ACHOTW_001234"
     CDS             complement(16910..18058)
                     /gene="galK"
                     /locus_tag="ACHOTW_001234"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9843380.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18062..19108)
                     /gene="galT"
                     /locus_tag="ACHOTW_001235"
     CDS             complement(18062..19108)
                     /gene="galT"
                     /locus_tag="ACHOTW_001235"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP9843381.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19118..20134)
                     /gene="galE"
                     /locus_tag="ACHOTW_001236"
     CDS             complement(19118..20134)
                     /gene="galE"
                     /locus_tag="ACHOTW_001236"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9843382.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20396..21868)
                     /gene="modF"
                     /locus_tag="ACHOTW_001237"
     CDS             complement(20396..21868)
                     /gene="modF"
                     /locus_tag="ACHOTW_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP9843383.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            complement(21936..22724)
                     /gene="modE"
                     /locus_tag="ACHOTW_001238"
     CDS             complement(21936..22724)
                     /gene="modE"
                     /locus_tag="ACHOTW_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP9843384.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            22853..23002
                     /gene="acrZ"
                     /locus_tag="ACHOTW_001239"
     CDS             22853..23002
                     /gene="acrZ"
                     /locus_tag="ACHOTW_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP9843385.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23169..23942
                     /gene="modA"
                     /locus_tag="ACHOTW_001240"
     CDS             23169..23942
                     /gene="modA"
                     /locus_tag="ACHOTW_001240"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9843386.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            23942..24631
                     /gene="modB"
                     /locus_tag="ACHOTW_001241"
     CDS             23942..24631
                     /gene="modB"
                     /locus_tag="ACHOTW_001241"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP9843387.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24634..25692
                     /gene="modC"
                     /locus_tag="ACHOTW_001242"
     CDS             24634..25692
                     /gene="modC"
                     /locus_tag="ACHOTW_001242"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP9843388.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(25693..26511)
                     /gene="ybhA"
                     /locus_tag="ACHOTW_001243"
     CDS             complement(25693..26511)
                     /gene="ybhA"
                     /locus_tag="ACHOTW_001243"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP9843389.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            26666..27661
                     /gene="pgl"
                     /locus_tag="ACHOTW_001244"
     CDS             26666..27661
                     /gene="pgl"
                     /locus_tag="ACHOTW_001244"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP9843390.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(27702..28655)
                     /gene="ybhD"
                     /locus_tag="ACHOTW_001245"
     CDS             complement(27702..28655)
                     /gene="ybhD"
                     /locus_tag="ACHOTW_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9843391.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            28839..29891
                     /gene="ybhH"
                     /locus_tag="ACHOTW_001246"
     CDS             28839..29891
                     /gene="ybhH"
                     /locus_tag="ACHOTW_001246"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP9843392.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            29967..31400
                     /gene="ybhI"
                     /locus_tag="ACHOTW_001247"
     CDS             29967..31400
                     /gene="ybhI"
                     /locus_tag="ACHOTW_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP9843393.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            31583..33844
                     /gene="ybhJ"
                     /locus_tag="ACHOTW_001248"
     CDS             31583..33844
                     /gene="ybhJ"
                     /locus_tag="ACHOTW_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP9843394.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGSSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(33985..35268)
                     /gene="ybhC"
                     /locus_tag="ACHOTW_001249"
     CDS             complement(33985..35268)
                     /gene="ybhC"
                     /locus_tag="ACHOTW_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP9843395.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35403..36469)
                     /locus_tag="ACHOTW_001250"
                     /pseudo
     CDS             complement(35403..36469)
                     /locus_tag="ACHOTW_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(36447..36665)
                     /locus_tag="ACHOTW_001251"
     CDS             complement(36447..36665)
                     /locus_tag="ACHOTW_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9843396.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(36705..36872)
                     /locus_tag="ACHOTW_001252"
     CDS             complement(36705..36872)
                     /locus_tag="ACHOTW_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843397.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            37115..37717
                     /locus_tag="ACHOTW_001253"
     CDS             37115..37717
                     /locus_tag="ACHOTW_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843398.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(37928..38149)
                     /locus_tag="ACHOTW_001254"
     CDS             complement(37928..38149)
                     /locus_tag="ACHOTW_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9843399.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(38248..38463)
                     /locus_tag="ACHOTW_001255"
     CDS             complement(38248..38463)
                     /locus_tag="ACHOTW_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9843400.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(38540..38731)
                     /locus_tag="ACHOTW_001256"
     CDS             complement(38540..38731)
                     /locus_tag="ACHOTW_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9843401.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            complement(38704..38886)
                     /locus_tag="ACHOTW_001257"
     CDS             complement(38704..38886)
                     /locus_tag="ACHOTW_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9843402.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            complement(38883..39563)
                     /locus_tag="ACHOTW_001258"
     CDS             complement(38883..39563)
                     /locus_tag="ACHOTW_001258"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9843403.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(39560..40231)
                     /gene="bet"
                     /locus_tag="ACHOTW_001259"
                     /pseudo
     CDS             complement(39560..40231)
                     /gene="bet"
                     /locus_tag="ACHOTW_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            40261..40443
                     /locus_tag="ACHOTW_001260"
     CDS             40261..40443
                     /locus_tag="ACHOTW_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9843404.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            40440..40610
                     /locus_tag="ACHOTW_001261"
     CDS             40440..40610
                     /locus_tag="ACHOTW_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP9843405.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            40603..41223
                     /locus_tag="ACHOTW_001262"
     CDS             40603..41223
                     /locus_tag="ACHOTW_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9843406.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVHAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELIS
                     RIGQEAVEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            41220..41885
                     /locus_tag="ACHOTW_001263"
     CDS             41220..41885
                     /locus_tag="ACHOTW_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFP9843407.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            41882..42505
                     /locus_tag="ACHOTW_001264"
     CDS             41882..42505
                     /locus_tag="ACHOTW_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9843408.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            42758..>43419
                     /locus_tag="ACHOTW_001265"
     CDS             42758..>43419
                     /locus_tag="ACHOTW_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843409.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SF"
BASE COUNT        10524 a        10464 c        11234 g        11197 t
ORIGIN      
        1 aatttggttt attcattaac atatttgtaa cttttttatc attgtctttt cctcttttga
       61 tgaaatggca acgatagagg cgagcaaata attttgtatt ggggcatgcg tgtgaccctt
      121 tctaacgggg ttcactctcg gagtcttcat gcgatgagca aggagtcatg atgttagata
      181 tagtcgaact gtcgcgctta cagtttgcct tgaccgcgat gtaccacttc ctttttgtgc
      241 cactgacgct cggtatggcg ttcctgctgg ccattatgga aacggtctac gtcctttccg
      301 gcaaacagat ttataaagat atgaccaagt tctggggcaa gttgtttggt atcaacttcg
      361 ctctgggtgt ggctaccggt ctgaccatgg agttccagtt cgggactaac tggtcttact
      421 attcccacta tgtaggggat atcttcggtg cgccgctggc aatcgaaggt ctgatggcct
      481 tcttcctcga atccaccttt gtaggtctgt tcttcttcgg ttgggatcgt ctgggtaaag
      541 ttcagcatat gtgtgtcacc tggctggtgg cgctcggttc aaacctgtcc gcactgtgga
      601 ttctggttgc gaacggctgg atgcaaaacc caatcgcgtc cgatttcaac tttgaaacta
      661 tgcgtatgga gatggtgagc ttctccgagc tggtgcttaa cccggttgct caggtgaaat
      721 tcgttcacac tgtagcgtct ggttatgtga ctggcgcgat gttcatcctc ggtatcagcg
      781 catggtatat gctgaaaggt cgtgacttcg ccttcgctaa acgctccttt gctatcgctg
      841 ccagcttcgg tatggctgcc gttctgtctg ttattgttct gggtgatgaa tccggctacg
      901 aaatgggcga cgtgcagaaa accaaactgg ctgctattga agctgagtgg gaaacgcaac
      961 ctgcgcctgc tgcctttact ctgttcggca ttcctgatca ggaagaggag acgaacaaat
     1021 ttgcgatcca gatcccttac gcgctgggca taattgcaac gcgttccgtg gatactccag
     1081 ttatcggcct gaaagaactg atggtgcagc atgaagaacg cattcgtaac gggatgaagg
     1141 cgtactctct gctcgaacaa ctgcgttctg gttctaccga ccaggcggtt cgtgaccagt
     1201 tcaatagcat gaagaaggac ctcggttacg gtctgctgct gaaacgctat acgccaaacg
     1261 tggctgatgc gactgaagcg cagattcaac aggcaaccaa agactccatt ccgcgtgtag
     1321 cgccgctgta cttcgcgttc cgtatcatgg tggcgtgtgg cttcctgctg ctggcaatca
     1381 tcgcgctctc tttctggagt gtcatccgca accgcattgg cgagaaaaaa tggctcctgc
     1441 gcgccgcgct gtacggtatt ccgctgccgt ggattgctgt agaagcgggc tggttcgtgg
     1501 ctgaatatgg ccgccaaccg tgggctatcg gtgaagtgct gccgacagct gtggcgaact
     1561 cgtcactgac cgcaggcgat ctcatcttct caatggtgct gatttgcggc ctgtataccc
     1621 tgttcctggt ggcagaattg ttcttaatgt tcaagtttgc acgcctcggc ccaagcagcc
     1681 tgaaaaccgg tcgctatcac tttgagcagt cttccacgac tactcagccg gcacgctaag
     1741 acaggagtcg tcaaatgatc gattatgaag tattgcgttt tatctggtgg ctgctggttg
     1801 gcgttctgct gattggtttt gcagtcactg atggtttcga catgggggtg ggcatgctca
     1861 cccgtttcct cggtcgtaac gacaccgagc gtcgaattat gattaactcc atcgcaccac
     1921 actgggacgg taaccaggtt tggctgatca ccgcaggcgg cgcactgttt gctgcctggc
     1981 cgatggtcta tgccgctgcg ttctccggct tctatgtggc gatgatcctc gtgctggcgt
     2041 ctttgttctt ccgtccggtc ggttttgact accgctccaa gattgaagaa acccgctggc
     2101 gtaacatgtg ggactggggc atcttcattg gtagcttcgt tccgccgttg gtgattggtg
     2161 tagcgttcgg taacctgttg cagggcgtac cgttcaacgt tgatgaatat ctgcgtctgt
     2221 actacaccgg taacttcttc cagctgctta acccgttcgg cctgctggca ggcgtggtga
     2281 gcgtagggat gatcattact cagggcgcaa cctatctgca aatgcgtacc gtgggcgaac
     2341 tgcacctgcg tacccgtgca acggctcagg tggcggcact ggtgacactg gtctgtttcg
     2401 cactggctgg cgtatgggtg atgtacggta tcgatggtta tgtcgtgaaa tcgacaatgg
     2461 accattacgc agcctctaac ccgctgaata aagaagtggt tcgtgaagct ggcgcatggc
     2521 tggttaactt caacaacacg ccaattctgt gggctattcc ggcactgggt gtggttctgc
     2581 cgctgctgac catcctgact gcacgtatgg ataaagccgc gtgggcgttt gtgttctcct
     2641 ccctgacgct ggcctgcatc atcctgactg ccggtatcgc aatgttcccg tttgtgatgc
     2701 cgtccagcac catgatgaac gcaagtctga caatgtggga tgcaacttcc agccagctga
     2761 cacttaacgt catgacctgg gttgcggtgg ttctggtacc gatcattctg ctctacaccg
     2821 cctggtgtta ctggaaaatg ttcggtcgta tcaccaaaga agatattgaa cgtaacaccc
     2881 actctctgta ctaagtaagg agctaaaaat gtggtatttc gcatggattc tgggaacgct
     2941 tcttgcctgt tcgtttgggg taatcaccgc gctggcgctt gagcacgtcg aatcaggcaa
     3001 agccggtcaa gaagacatct gatgagtaag attatcgcaa ccttgtatgc ggtaatggac
     3061 aagcgccccc tgcgggcgct ttccttcgtg atggcgcttc tgttagcagg atgtatgttt
     3121 tgggacccat ctcgtttcgc cgcgaagacc agtgaactgg aaatctggca tggtttattg
     3181 ctgatgtggg ccgtctgtgc tggtgtgatt cacggcgtgg gctttcgtcc gcagaaggtt
     3241 ctttggcaag ggattttttg cccattgctt gccgatattg ttctcattgt cgggctgatt
     3301 ttcttcttct tttaaatcag aattctcttt aaaaaattat gggccgtgcc aggcccataa
     3361 atttttacgc tcccttaact tgccctcatt cccaaacctc aatcgcgcgc gtatagtagc
     3421 agcgtttaaa agctctaact tttgttgcat taccgggatg taaagtgaat acaacgctgt
     3481 ttcgatggcc ggttcgcgtc tactatgaag ataccgatgc cggtggtgtg gtgtaccacg
     3541 ccagttacgt cgctttttat gaaagagcac gcacagagat gctgcgtcat catcacttca
     3601 gtcaacaggc gctgatggct gaacgcgttg cctttgtggt acgtaaaatg acggtggaat
     3661 attacgcacc tgcgcggctc gacgatatgc tcgaaataca gactgaaata acatcaatgc
     3721 gtggcacctc tttggttttc acgcaacgta ttgtcaacgc cgagaatact ttgctgaatg
     3781 aagcagaggt tctggttgtt tgcgttgacc cactcaaaat gaagcctcgt gcgcttccca
     3841 agtctattgt cgcggagttt aagcagtgac tgacatgaat atccttgatt tgttcctgaa
     3901 ggctagcctt ctggttaaac ttatcatgtt gattttgatt ggtttttcaa tcgcatcctg
     3961 ggccattatt atccagcgga cccgtattct taacgcagcg gcgcgcgaag ccgaagcgtt
     4021 tgaagataaa ttctggtctg gaatcgaact ctctcgcctc tatcaagaga gccaggggaa
     4081 acgggataat ctgactggtt cggaacaaat cttttacagc gggttcaaag aatttgtgcg
     4141 cctgcatcgt gccaatagcc atgcgccgga agccgtagtg gaaggggcgt cgcgtgcgat
     4201 gcgtatttcc atgaaccgcg aacttgaaaa tctggaaacg cacattccgt tcctcggtac
     4261 ggttggctcc atcagcccgt atattggtct gtttggtacg gtctggggga tcatgcacgc
     4321 ctttatcgcc ctcggggcgg taaaacaagc aacactgcaa atggttgcgc ccggtatcgc
     4381 agaagcgttg attgcgacgg caattggtct gttcgccgct attccggcgg taatggctta
     4441 taaccgtctc aaccaacgtg taaacaaact ggaactgaat tacgacaact ttatggaaga
     4501 gtttaccgcg attctgcacc gccaggcgtt taccgttagc gagagcaaca aggggtaagc
     4561 catggccaga gcgcgtggac gaggtcgtcg cgatctcaag tccgaaatca acattgtacc
     4621 gttgctggac gtactgctgg tgctgttgct gatctttatg gcgacagcgc ccatcatcac
     4681 ccagagcgtg gaggtcgatt tgccagacgc tactgaatca caggcggtga gcagtaacga
     4741 taatccgcca gtgattgttg aagtgtctgg tattggtcag tacaccgtgg tggttgagaa
     4801 agatcgcctg gagcgtttac caccagagca ggtggtggcg gaagtgtcca gccgtttcaa
     4861 ggccaacccg aaaacggtct ttctgatcgg tggcgcaaaa gatgtgcctt acgatgaaat
     4921 aattaaagca ctgaacttgt tacatagtgc gggtgtgaaa tcggttggtt taatgacgca
     4981 gcctatctaa acatctgcgt ttcccttgct tgaaagagag cgggtaacag gcgaacagtt
     5041 tttggaaacc gagagtgtca aaggcaaccg aacaaaacga caagctcaag cgggcgataa
     5101 ttatttcagc agtgctgcat gtcatcttat ttgcggcgct gatctggagt tcgttcgatg
     5161 agaatataga agcttcagct ggaggcggcg gtggttcgtc catcgacgct gtcatggttg
     5221 attcaggtgc ggtagttgag cagtacaaac gcatgcaaag ccaggaatca agcgcgaagc
     5281 gttctgatga gcagcgcaag atgaaggaac agcaggctgc tgaagaactg cgtgagaaac
     5341 aagcggctga acaggaacgc ctgaagcaac ttgagaaaga gcggttagct gctcaggaac
     5401 agaaaaagca ggctgaagaa gccgcaaaac aggccgagtt aaagcagaag caagcggaag
     5461 aggcggcagc gaaagcggcg gcagatgcta aagcgaaggc cgaagcggat gataaagctg
     5521 cggaagaagc agcgaagaaa gcggctgcag acgcgaagaa aaaagcagaa gcagaagccg
     5581 ccaaagccgc agccgaagcg cagaaaaaag ccgaggcagc agctgcggcg ctgaagaaga
     5641 aagcggaagc ggcagaagca gctgcagctg aagcaagaaa gaaagcggca gcagagaaag
     5701 ctgcagccga caaaaaagca gcagaaaaag cggctgctga aaaggcagca gctgataaga
     5761 aagcagcggc agaaaaagcc gccgcagaca aaaaagcggc agctgcaaaa gcagcagctg
     5821 aaaaagccgc tgcagcaaaa gctgccgcgg aggcagatga tattttcggt gagctaagct
     5881 ctggtaagaa tgcaccgaaa acggggggag gggcgaaagg gaacaatgct tcgcctgccg
     5941 ggagtggtaa tactaaaaac aatggcgcat caggggccga tatcaataac tatgccgggc
     6001 agattaaatc tgctatcgaa agtaagttct atgacgcatc gtcctatgca ggcaaaacct
     6061 gtacgctgcg cataaaactg gcacccgatg gtatgttact ggatatcaaa cctgaaggtg
     6121 gcgatcccgc actttgtcag gctgcgttgg cagcagctaa acttgcgaag atcccgaaac
     6181 caccaagcca ggcagtatat gaagtgttca aaaacgcgcc attggacttc aaaccgtaat
     6241 cgcgatgttg actgttccga cggtcaacat caggcaccgg ttgccacggg gttctggtag
     6301 ttttgtgtat tttagtttgt taacattctg ctaaattatc gtgggccatc ggtccagata
     6361 agggagatat gatgaagcag gcattacgag tagcatttgg ttttctcata ctgtgggcat
     6421 cagttctgca tgctgaagtc cgcattgtga tcgacagcgg tgtagattcc ggtcgtccta
     6481 ttggagttgt tcctttccag tgggcgggcc ctggtgcggc acctgaagat attggcggca
     6541 tcgttgctgc cgacttgcgt aacagcggta aatttaatcc gttagatcgc gctcgtctgc
     6601 cacagcagcc gggtagtgcg caggaagtac aaccagctgc atggtccgcg ctgggtattg
     6661 acgctgtagt tgtcggtcag gtcactccga atccggatgg ttcttacaat gttgcttatc
     6721 aacttgttga cactggcggc gcaccgggta ctgtacttgc tcagaactcg tacaaagtga
     6781 acaagcagtg gctgcgttat gctggtcata ccgccagtga tgaagtgttt gaaaaactga
     6841 ccggtattaa aggtgcgttc cgtacccgta ttgcctacgt tgttcagacc aacggcggtc
     6901 agttcccgta tgaactgcgc gtatctgact atgacggtta caaccagttt gtcgttcacc
     6961 gttcaccaca gccgctgatg tccccggcgt ggtcaccaga cggttctaaa ctggcttatg
     7021 tgaccttcga aagcggtcgt tccgcgctgg ttatacaaac gctggcaaac ggcgctgtac
     7081 gtcaggtggc ttcattcccg cgtcacaacg gtgcgcctgc attctcgcca gacggcagca
     7141 aactggcatt cgccttgtcg aaaaccggta gcctgaacct gtacgtaatg gatttggctt
     7201 ctggtcagat ccgccaggtg actgatggtc gcagtaacaa taccgaaccg acctggttcc
     7261 cggacagcca gaacctggca tttacttctg accaggccgg tcgtccacag gtttataaag
     7321 tgaatatcaa cggcggtgcg ccacaacgta ttacctggga aggttcgcag aaccaggatg
     7381 cggatgtcag cagcgacggt aaatttatgg taatggtcag ctccaatggt gggcagcagc
     7441 acattgccaa acaagatctg gcaacgggag gcgtacaagt tctgtcgtcc acgttcctgg
     7501 atgaaacgcc aagtctggca cctaacggca ctatggtaat ctacagctct tctcagggga
     7561 tgggatccgt gctgaatttg gtttctacag atgggcgttt caaagcgcgt cttccggcaa
     7621 ctgatggaca ggtcaaattc cctgcctggt cgccgtatct gtgataataa ttaattgaat
     7681 agtaaaggaa tcattgaaat gcaactgaac aaagtgctga aagggctgat gattgctctg
     7741 cctgttatgg caattgcggc atgttcttcc aacaagaacg ccagcaatga cggcagcgaa
     7801 ggcatgctgg gtgccggcac tggtatggat gcgaacggcg gcaacggcaa catgtcttcc
     7861 gaagagcagg ctcgtctgca aatgcaacag ctgcagcaga acaacatcgt ttacttcgat
     7921 ctggacaagt acgatatccg ttctgacttc gctcaaatgc tggatgcaca tgcaaacttc
     7981 ctgcgtagca acccgtctta caaagtcacc gtagaaggtc acgcggacga acgtggtact
     8041 ccggaataca acatctccct gggtgaacgt cgtgcgaacg ccgttaagat gtacctgcag
     8101 ggtaaaggcg tttctgcaga ccagatctcc atcgtttctt acggtaaaga aaaacctgca
     8161 gtactgggtc atgacgaagc ggcatactcc aaaaaccgtc gtgcggtact ggtttactaa
     8221 gagaattgca tgagcagtaa cttcagacat caactattga gtctgtcgtt actggttggt
     8281 atagcggccc cctgggccgc ttttgctcag gcaccaatca gtagtgtcgg ctcaggctcg
     8341 gtcgaagacc gcgtcattca acttgagcgt atttctaatg ctcacagcca gcttttaacc
     8401 caactccagc aacaactctc tgataatcaa tccgatattg attccctgcg tggtcagatt
     8461 caggaaaatc agtatcaact gaatcaggtc gtggagcggc aaaagcagat cctgttgcag
     8521 atcgacagcc tcagcagcgg tggtgcagcg gcgcaatcaa ccagcggtga tcaaagcggt
     8581 gcggcggcat caacgacgcc gacagctgat gctggtactg cgaatgctgg cgcgccggtg
     8641 aaaagcggtg atgcaaacac ggattacaat gcagctattg cgctggtgca ggataaatcc
     8701 cgccaggatg acgcaatggt ggcatttcag aatttcatca aaaattaccc tgattcaact
     8761 tacctgccaa acgccaatta ttggctgggt cagttaaact acaacaaggg taaaaaagat
     8821 gatgcggcgt actattttgc ttcggtagtg aaaaactatc cgaagtcacc aaaggctgca
     8881 gatgcgatgt ttaaagtcgg cgtcatcatg caggacaaag gtgacaccgc aaaagcgaaa
     8941 gccgtatacc agcaggttat cagtaaatac cctggtactg atggcgctaa acaggcacaa
     9001 aaacgtctga acgcgatgta atgcataaca cacgaccaga agtcgcatta tttctggtcg
     9061 tgtcgtgcga atcataagca gttgagtgat ctacatcgaa atttttgttg cgctcaagtc
     9121 tgaaatcagt aatatatgcc gccgttgcca cgggatatca aacaaaccga aagcaacgaa
     9181 aaagtgggtc gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt
     9241 cgaatcctgc acgacccacc actaacctag ttagttgtag tatccagcgt aatatcgggt
     9301 gattagctca gctgggagag cacctccctt acaaggaggg gggcggcggt tcgatcccgt
     9361 catcacccac caccgggtcg ttagctcagt tggtagagca gttgactttt aatcaattgg
     9421 tcgcaggttc gaatcctgca cgacccacca ctaattacgg tgggttcggt ggaagtagtt
     9481 tgtagtatcc agcgcagtat cgggtgatta gctcagctgg gagagcacct cccttacaag
     9541 gaggggggcg gcggttcgat cccgtcatca cccaccactc gggtcgttag ctcagttggt
     9601 agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac ccaccaattt
     9661 taacatcaaa ctcagatgtt aagcgtgaag gataacgttg cgtcagcaac ggcccgtagg
     9721 gcaagcgaag cgagtcatcc tgcacgaccc accaatttta aagattggtt ccgagtagaa
     9781 atctttcagg taacacccgt atgggtcgtt agctcagttg gtagagcagt tgacttttaa
     9841 tcaattggtc gcaggttcga atcctgcacg acccaccaat gtaaaaaagc gccctaaagg
     9901 cgcttttttg ctattcaggc atctctcaat ttcaattctt gaccctgatg acatactccg
     9961 cgcttgctgc gtaaacaact ttacgtcgga ggaataagac gcaaagtgtg gcattcggca
    10021 tctcatacgt aattcgagtg acttttcccc accattcgac tatcttgttt agcatataaa
    10081 acaaattaca ccgataacag cgaatatcac gctaatgtcg gttttaacgt taagcctgta
    10141 aaacgagatg gtaagatgag cgtgatgttt gatccagaca cggcgattta tcctttcccc
    10201 ccgaagccga cgccgttaag cattgatgaa aaagcgtatt accgcgagaa gataaaacgt
    10261 ctgctaaaag aacgtaatgc ggtgatggtt gcccactact ataccgatcc cgaaattcaa
    10321 caactggcag aagaaaccgg tggctgtatt tctgattctc tggaaatggc gcgcttcggt
    10381 gcaaagcatc ccgcttctac gttgttagtc gctggggtga gatttatggg agaaaccgcc
    10441 aaaattctca gtccggaaaa aacaattctg atgccgacac ttcaggctga gtgttcactg
    10501 gatctcggct gccctgttga agaatttaac gcattttgcg atgcccatcc cgatcgtact
    10561 gtcgtcgtct acgccaacac ttctgctgcg gtaaaagcgc gggcagattg ggtggtaact
    10621 tcaagcattg ccgtcgaact tattgatcat ctggatagtt tgggtgaaaa aatcatctgg
    10681 gcacccgaca aacatctggg gcgttacgtg caaaaaaaga cgggcgcaga cattttatgc
    10741 tggcagggtg cctgcattgt gcatgatgag tttaagactc aggcgttaac ccgcttgcaa
    10801 gaagaatacc cggatgctgc catactggtg catccagaat caccacaagc tattgtcgat
    10861 atggcggatg cggtcggttc caccagtcaa ctgatcgctg ctgcgaaaac attgccacat
    10921 cagaggctta ttgtggcaac cgatcggggt attttctaca aaatgcagca ggcggtgcca
    10981 gataaagagt tactggaagc accaaccgcg ggtgagggcg caacctgccg cagctgcgcg
    11041 cattgtccgt ggatggccat gaatggcctc caggccatcg ctgaggcatt agaacaggaa
    11101 ggaagcaatt acgaggttca tgttgatgaa aggctgcgag agagggcgct ggtgccgctc
    11161 aatcgtatgc tggattttgc ggctacacta cgtggataac gaataataag gcgtaacgtt
    11221 acgctttggg ggaaagatgg atttttttag tgtgcagaat atcctggtac atataccaat
    11281 aggagcaggc ggttatgatc tctcatggat cgaagcggta ggcacaatcg ccgggttgct
    11341 gtgtattggc cttgccagtc tggagaagat cagcaactac ttctttggcc tgatcaacgt
    11401 caccttgttt ggcattattt tctttcagat tcagctgtat gccagcctgc tattacaggt
    11461 gtttttcttt gccgcgaata tttacggctg gtatgcgtgg tcgcgacaaa ccagtcagaa
    11521 cgaggcggag ttaaaaattc gctggttgcc attgccgaag gcactcagct ggttggcggt
    11581 ttgcgttgtt tcgattggtc tgatgacggt atttatcaat ccggtgtttg catttttgac
    11641 ccgcgtggca gtcatcatca tgcaagcatt aggattacag gttgcgatgc ctgaactaca
    11701 accggacgct ttcccgttct gggattcttg catgatggtg ttatctatcg tggcaatgat
    11761 tctgatgact cgtaagtatg tggaaaactg gctgttgtgg gtgattatta acgtgattag
    11821 cgtcgttatt tttgcacttc agggcgttta cgccatgtct ctggagtaca tcatcctgac
    11881 ctttattgcg ctcaacggca gccggatgtg gatcaatagc gcacgcgaaa gaggctcacg
    11941 cgcgctgtcc cattaatggt gatgatgtga atggccagac accccttcat tcaggtggca
    12001 gtctggccca tgacaaggtt gatattccat ctgaatggtg gcgtgctcaa tctgatagtg
    12061 atccatcagg tagtgttgga tctgatccaa caaggcatcg tgatcgtgtg gcgggatcac
    12121 ctgcacatgc aacgtcatca ccggcttctc gcctaccatc catacatgca catggtgcac
    12181 attgcggact tccgggattt cccggcacat acgacgcttc agttcagcga tatccagcga
    12241 taccggtgca ccctcaagta attcattcac gctatctttc aacagtcgcc acgcgctacg
    12301 cagaaccaga agcgacacca gtatcgagag aatggggtca gcaggtgtcc agccggtcca
    12361 gataataatc aacgcggcaa taatagcccc aaccgaaccc agcaggtccc cgagcacatg
    12421 cagtgccgct gctcgtacgt tgaggttttt ctcttcgctg ccgtgatgaa gtaaccagaa
    12481 agaaagtata tttgccagca acccagccac ggcaattgcc atcatcatgc cgccctcgac
    12541 cggacgcggc gtgcggaagc gttctatcgc ctcccagaca atcaaaatgg taattaccac
    12601 gagggcgatg gcattcacaa acgccgcgag cgtggtcaat cttagccaac cgaaagtgtg
    12661 gcgaatggtg ggaggacgac gtgaaaattg cacggcgagt agggcgaaaa gcagggcggc
    12721 agtatcggtc aacatatgac ccgcatcggc cagcaaagcc agagaaccgg aaagaaaacc
    12781 accaatgact tctaccagca taaacccggc agtcacgccg aaagcataca acaagcgacg
    12841 agcattatta tcttctggca ggtgtgagga cgtgtgtgag tgtgagtgcg ccatgacgag
    12901 tcatccctta attattctca cttaacttta tgacatcata ctgcttttag aagggaaaaa
    12961 ttaaaaggga gagactccgc tctcccatta ttggctattt tgcagggtta ctgcgtggta
    13021 ccgtcggttt tggtatcgac atcattattg atgccatcac cggtttgtac ctttttattg
    13081 atatccgggc agcgaccatc tttgcacatg gtgttcttgt gctcttcatc tttggtcatg
    13141 ccgtcattgt tcattgaaga accatccgga tgcagcatag tgccgccaga accggtattt
    13201 accccgttat tgtcgacgtt atttggcgcg acattttcac gggcgtcagg ggctacctgg
    13261 cccgcatcag ctgcggcgtt tgcctggccg ttattggttt gcgctccgct atcggcagcc
    13321 agtgcggcac cgctggcaag gcttagagtg gcagtcagaa atagtgtggc cagttttgtc
    13381 attttcatag gatgctcctg ttatggtcgt tatgtcggat aacctcttcc aacagtgcat
    13441 ttgcaggtga atataaggca ttggtttaag atttcagcca ggcaaagaaa tccagcagag
    13501 aatcttgaaa taattaacaa acaaaggagt tacagttaga aattgtagga gagatctcgt
    13561 ttttcgcgac aatctggcgt ttttcttgct aattccagga ttaatccgtt catagtgtaa
    13621 aaccccgttt acacattctg acggaagata tagattggaa gtattgcatt cactaagata
    13681 agtatggcaa cactggaaca gacatgaatt atcagaacga cgatttacgc atcaaagaaa
    13741 tcaaagagtt acttcctcct gtcgcattgc tggaaaaatt ccccgctact gaaaatgccg
    13801 cgaatacggt tgcccatgcc cgaaaagcga tccataagat cctgaaaggt aatgatgatc
    13861 gcctgttggt ggtgattggc ccgtgctcaa ttcatgatcc tgtcgcggct aaagagtatg
    13921 ccactcgctt gctggcgctg cgtgaagagc tgaaagatga gctggaaatc gtaatgcgcg
    13981 tctattttga aaagccgcgt actacggtgg gctggaaagg gctgattaac gatccgcata
    14041 tggataacag cttccagatc aacgacggtc tgcgtatagc ccgtaaattg ctgcttgata
    14101 ttaacgacag cggtctgcca gcggcgggtg aattcctcga tatgatcact cctcagtatc
    14161 tcgctgacct gatgagctgg ggcgcaattg gtgcacgtac cacggaatcg caggtgcacc
    14221 gcgaactggc gtctggtctt tcttgtccgg taggttttaa aaatggcaca gacggtacga
    14281 ttaaagtggc tatcgatgcc attaatgccg ccggtgcgcc gcactgcttc ctgtccgtaa
    14341 ctaaatgggg gcattcggcg attgtgaata ccagcggtaa cggcgattgc catatcattc
    14401 tgcgcggcgg taaagagcct aactacagcg cgaagcacgt tgctgaagtg aaagaagggc
    14461 tgaacaaagc aggtctgcca gctcaggtga tgatcgattt cagccatgct aattcgtcca
    14521 aacaattcaa aaagcagatg gatgtttgtg ctgacgtttg ccagcagatt gccggtggcg
    14581 aaaaggccat tatcggtgtg atggtggaaa gtcatctggt ggaaggcaat cagagcctgg
    14641 agagcgggga gccgctggcc tatggtaaga gcatcaccga tgcctgcatt ggctgggaag
    14701 ataccgatgc tctgttacgt caactggcga acgcagtgaa agcgcgtcgc gggtaaagtt
    14761 taattgtcgg atgcgccgtc agagtggcgt atccgatgaa tcaccgtagc cctgacaaga
    14821 cgcgcagcgt cgcatcaggc aatgtgctcc attgttagcg acgacaaaca aaaaagccga
    14881 ctcacttgca gtcggctttc tcatttttaa acgaatgacg tttacttcgc tttaccctgg
    14941 ttcgcaacgg ctgctgcttt cgccgcgatc tcgtcagcat tacccagata atagcgtttc
    15001 agcggtttga agttctcgtc aaactcataa accagcggca cgccggtcgg gatattcagc
    15061 tcaagaatct cttcttcgct catgttatcg agatatttca ccagcgcacg taacgagtta
    15121 ccgtgtgcag cgatgattac gcgctcaccg ctcttcatac gcggcagaat ggtttcattc
    15181 cagtaaggga tcacgcggtc aatggtcagc gccaggcttt ccgtcagcgg cagttctttc
    15241 tcgctcagtt tcgcgtaacg cggatcgtga cccggataac gctcatcatc tttagtcagt
    15301 tccggcggag tcactgcaaa accacgacgc cactgtttca cctgctcgtc gccatatttt
    15361 tcagcagttt ccgctttgtt cagaccctgc aacgcaccgt agtgacgttc gttcagtttc
    15421 caggatttct caacgggcag ccatgcctga tccagttcgt ccagcacatt ccacagggta
    15481 tggatagcgc gtttcagcac agaggtgtaa gcaaagtcaa agctgtaacc ttcctctttc
    15541 agcagcttac ctgctgcttt tgcttcgctt acgcctttct cagacagatc cacgtcgtac
    15601 caaccggtga aacggttttc tttgttccac tgactttcgc catgacgaac cagaaccagc
    15661 ttagttacag ccatatactt actcctcaaa tcatctttta atgataataa ttctcattat
    15721 attgccgtga cgaagcaaca gcaatgctta cgcataacca tagcgaaaat agtggcgcag
    15781 tgtaaggttg ttgtgaatat tgagttgcaa atatgtcggt gtttgctggt gatttgaaca
    15841 atatgagata aagccctcat gacgagggct taacattact cagcaataaa ctgatattcc
    15901 gtcaggctgg aatactcttc accaggatgc aggaaacagt ccggttgcgg ccattcaggg
    15961 tggttcgggc tgtccggcag aaactcgctt tccaacgcca gcccttgcca gtcggcgtaa
    16021 ggttcggttc cccgcgacgg tgtgccgccg aggtagttgc cggagtagaa ttgcagagcc
    16081 ggagcggtgg tgtagacctt cagctgcaat ttttcatctg ctgaccagac atgcgccgcc
    16141 actttcttgc catcgccttt ggcctgtaac aagaatgcgt gatcgtaacc tttcactttg
    16201 cgctgatcgt cgtcggcaag aaactcactg gcgatgattt tggcgctgcg gaaatcaaaa
    16261 gacgttccgg cgacagattt caggccgtcg tgcggaatac cgccttcatc aaccggcaga
    16321 tattcgtccg ccagaatctg caacttgtga ttgcgcacgt cagactgctt gccgtcaaga
    16381 ttgaaataga cgtgattagt catattcacc gggcaaggtt tatcaactgt ggcgcgataa
    16441 gtaatggaga tacggttatc gtcggtcaga cgatattgca ccgtcgcgcc gagattaccc
    16501 gggaagccct gatcgccatc atctgaactc agggcaaaca gcacctgacg atcgttctgg
    16561 ttcacaatct gccagcgacg tttgtcgaac ccttccgggc cgccgtgcag ctggttaacg
    16621 ccctgacttg gcgaaagcgt cacggtttca ccgtcaaagg tataacgact attggcgatg
    16681 cggttggcat aacgaccaat agaggcaccc agaaacgcgg cctgatcctg atagcattcc
    16741 gggctggcac agccgagcag cgcctcgcgg acgctgccat cggaaagcgg aatacgggct
    16801 gaaagtaaag tcgcacccca gtccatcagc gtgactacca tccctgcgtt gttacgcaaa
    16861 gttaacagtc ggtacggctg accatcgggt gccagtgcgg gagtttcgtt cagcactgtc
    16921 ctgctccttg tgatggttta caaacgtaaa aagtctcttt aatacctgtt tttgcttcat
    16981 attgttctgc gacagcttgc tgtacggcag gcaccagctc ttccgggatc agcgcgacga
    17041 tacagccgcc aaatccgccg ccggtcatgc gtacgccacc tttgtcgcca atcacagctt
    17101 tgacgatttc taccagagtg tcaatttgcg gcacggtgat ttcgaaatca tcgcgcatag
    17161 aggcatgaga ctccgccatc aactcgccca tacgtttcag gtcgccttgc tccagcgcgc
    17221 tggcagcttc aacggtgcgg gcgttttcag tcaggatatg acgcacgcgt ttcgccacga
    17281 ttgggtccag ctcatgtgca acagcgttga actcttcaat ggtgacatcg cgcagagctg
    17341 gctgctggaa gaaacgcgca ccggtttcgc actgttcacg acgggtgttg tattcgctgc
    17401 caaccagggt acgtttgaag ttactgttga tgatgacgac agccacacct ttcggcatgg
    17461 aaactgcttt ggtccccagt gagcgacagt caatcagcaa ggcatgatct ttcttgccga
    17521 gtgcggaaat tagctgatcc atgatcccgc agttacagcc aacaaactgg ttttctgctt
    17581 cctgaccgtt aagcgcgatt tgtgcgccgt ccagcggcag atgataaagc tgctgcaata
    17641 cggttccgac cgcgacttcc agtgaagcgg aagaacttaa cccggcaccc tgcggcacat
    17701 tgccgctgat caccatgtcc acaccgccga agctgttgtt acgcagttgc agatgtttca
    17761 ccacgccacg aacgtagttc gcccattgat agttttcatg cgcgacaatg ggcgcatcaa
    17821 gggaaaactc atcaagctga ttttcataat cggctgccat tacgcgaact ttacggtcat
    17881 cgcgtggcgc acagctgatc acggtttgat aatcaatcgc gcagggcaga acgaaaccgt
    17941 cgttgtagtc ggtgtgttca ccaatcaaat tcacgcggcc aggcgcctga atggtatgag
    18001 tggcagggta gccaaatgcg ttggcaaaca gagattgtgt tttttctttc agactcattt
    18061 cttacactcc ggattcgcga aaatggatat cgctgactgc gcgcaaacgc tctgctgcct
    18121 gttctgcggt caggtctcgc tgggtttctg ccagcatttc ataaccaacc ataaatttac
    18181 gtacggtggc ggagcgcaac agaggcggat aaaagtgcgc gtgcagctgc cagtgttgat
    18241 tctcttcgcc attaaacggt gcgccgtgcc agcccataga gtaggggaag gagcactgga
    18301 agaggttgtc ataacgactg gtcagctttt tcaacgccag tgccaaatcg ctgcgctggg
    18361 catcggtcaa atcggtgatc cgcaaaacgt gggctttggg cagaagtagc gtttcgaacg
    18421 gccaggcagc ccagtaaggc acaactgcta accagtgttc ggtttcgaca acggtacggc
    18481 taccgtctgc cagctcgcgc tgaacataat ccaccagcat tggcgatttc tgctcggcaa
    18541 aatattcttt ttgcaggcgg tcttcgcgct cagcttcgtt aggcaggaag ctatttgccc
    18601 aaatctgtcc gtgcggatgc gggttagagc agcccatcgc cgcacctttg ttttcaaaga
    18661 cctgcaccca cgggtatgtt ttccccagtt ctgcggtttg ctcctgccag gttttgacga
    18721 tttccgtcaa tgccgcaacg ctcagttctg gcagcgtttt actgtgatcc ggtgaaaagc
    18781 agatcacccg gctggtaccg cgcgcgctct ggcaacgcat caaaggatcg ttgctttctg
    18841 gcgcatctgg cgtgtcagac atcaaggccg caaagtcatt agtgaaaacg taagtcccgg
    18901 tgtaatcggg gtttttatcg cctgtcaccc gcacattacc tgcgcagagg aagcaatctg
    18961 gatcgtgcgc aggtaacacc tgtttggctg gcgtttcctg cgccccctgc caggggcgct
    19021 tagcgcggtg cggtgaaacc agaatccatt gcccggtgag ctggttgtag cggcgatgtg
    19081 gatgatcaac gggattaaat tgcgtcatgg tcgttcctta atcgggatat ccctgtggat
    19141 ggcgtgactg ccagtgccag gtgtcctgcg ccatttcatc gagtgtgcgc gttacgcgcc
    19201 agttcagttc acggtcggct ttgctggcgt ccgcccagta ggccggaagg tcgccctcgc
    19261 gacgcggtgc aaaatgatag ttaaccggtt tgccgcaggc tttgctgaag gcattaacca
    19321 cgtccagcac gctgctgcct acgccagcac cgaggttgta gatgtgtacg cctggcttgt
    19381 tcgccagttt ttccatcgcc acgacgtgac cgtccgccag atccattacg tggatgtaat
    19441 cgcgtacgcc agtaccgtct tcggtcggat aatcgttacc aaaaatcgcc agcgagtcgc
    19501 gacggcctac agcaacctgg gcgatgtatg gcataaggtt attcggaatg ccttgcggat
    19561 cttcgcccat atcgcccgac ggatgtgcgc caaccgggtt gaagtagcgc agcagggcaa
    19621 tgctccagtc cggctgggct ttttgcagat cggtgaggat ctgttccacc atcagcttgc
    19681 ttttgccgta agggctttgc ggtgtgccgg tcgggaagct ttcaacgtat ggaattttgg
    19741 gctgatcgcc ataaacggtg gcggaggagc taaaaataaa gtttttgacg ttagcggcgc
    19801 gcatggcgct aatcaggcgc agagtaccgt tgacattgtt gtcgtaatat tccagcggtt
    19861 tttgtaccga ttcgccaacg gctttcagcc cggcgaagtg gatcacggtg tcgatagcgt
    19921 gatcgtgcag gatctcggtc atcaacgctt cgttacggat atcgccttca acaaacgtcg
    19981 gatgtttgcc gcctaaacgc tcgataacag gcagtacgct gcgcttactg ttacagaggt
    20041 tatcaagaat gatgacatca tgaccgtttt gcagtaattg cacacaggta tgacttccaa
    20101 tgtaaccgct accaccagtg accagaactc tcataattcg ctccattagg cttatggtat
    20161 gaaataacca tagcataaca aagatgcgaa aagtgtgaca tggaataaat tagtggaatc
    20221 gtttacacaa gaatttagcc gttttttatg cgcgattaag tgattataaa acagagggat
    20281 tgtgaatgat tgcgcttttt atctgaaaaa aagacgcagt ttcatgcctg gatgcgtctg
    20341 accgtgtgcc ggataaagcg tttacgccgc atccggcaca gtcagcatta ccgacttagt
    20401 ttatttttgt cagcgcataa tgatagaggc caccgtccgg cacgaactcc agacgatggg
    20461 taatacaagc cggcgcatct tcagcatggt gcgaaacaaa caacaattgc gtttcacctt
    20521 cgctaatcag cacatcaaca aaacggcgaa taagctggcg attgagcgga tcaagcccct
    20581 gcaatggttc gtcgagaata agcaacgtcg gatgtttcac cagtgcgcgg acgatcagcg
    20641 ccagacgctg ctgtccccag gaaagactat ggaacggagc gtcagccgtg cgtttatcaa
    20701 tgccgagaat atccagccac tgctgcacca gtttttgctg gcgatccgaa acggcctgat
    20761 aaatgccaat cgaatcaaaa tagccagaaa gaatcacatt acgcacagta gtactgaccc
    20821 ggtaatccag atgcaaacta ctgctgacgt aaccgatatg ctttttgata tcccagatgg
    20881 tttcgccgct gccacgacgt cgtccgaaaa gcgtcaaatc gttgctgtaa ccttgcggat
    20941 gatcgccagt aatcaggctt aataacgtcg attttcccgc gccatttggc ccgacaattt
    21001 gccagtgttc acctggattc acctgccagc taaggttatt aagaatgggg cgatcgttat
    21061 aagaaaccac gccattattc agcacaatgc gcggttcgtt ggcgggtaag gcgtgacgtg
    21121 ctgaaggttc atccggctcc ggcagttgca caccttcaag ctgttcacta tgcgccagtt
    21181 gcgcgacgag tgcttgttgg agcagttcct ctttagcgcc agtttccgct aacgtgcaat
    21241 ccgccagcac gccagcaaac tggacaaact ccgggatctc atcgaagcga ttgagcacca
    21301 gtaccagagt aataccggac tgatgtaacg aggcgagtcg ctcagccagc tgctgacgtg
    21361 aggcaacatc caggccatcg aacggctcat caagaatcaa caagtcaggc tccgacatca
    21421 gcgcctgaca cagcagggtt tttcgcgtct cgccagtgga aaggtattta aagcgtcggt
    21481 cgaggagggg ggtaataccg aactgctgtg ccagttgcat gcagcgcggt gcatccttta
    21541 cttcatcctg aataatctca gccgtagtgc gtccggtgtc atcttcgcca gggccgagca
    21601 tatcggtgtt attccgctgc cattcgtcgc tgacgagctt ttgcaattgc tcgaaggaga
    21661 gacgagtgat gtgggaaaac tggctttgcc gttcaccttt caaaagcgga agttcccccg
    21721 ccagcgcgcg ggccagggcc gatttcccgc ttccattcga accgacaaac gcccaactat
    21781 cacccgcgtt taacgttagc tgaggcaatt gcagcgtttt tgtgtcgcta agacgaaacg
    21841 tgccttgcaa aatttgcaac gatgacattt tatatcccat tttgtgcagc gattactgac
    21901 agggatacgt gtttcataac aaattgtcaa cacgcttagc acagtgtggc gataatcacg
    21961 ctgtcggcat taaagtaggc cgtgacattc tgtccttgct gaagagaagt cgcttcattt
    22021 accggcactg tggcgcacag tgtttgcccg tccggtagcg ccattaatac ttcgcactgc
    22081 tctgcgccgc gctcaatatg actaataata cccggtaatt ggttgtcagc gttttgcgcg
    22141 accgcttcgt cctgagtaat gcctacccac ggcgctttta gcaatatcaa cacttctttg
    22201 ccttcatcca gccccagacg cgcgccgctt tgtgcggtaa ttgcgacttt caggcgtgtt
    22261 tttccatcag ccagtaagac atcaacatgc tgttgaacgt catcatgatc gcgggcggtg
    22321 atggtaccga accactggtt acgggcgctg gtttggagtg aaaaacgtga gatcgcggcc
    22381 agcaggctgt tcagcggcag ggcgtcatcg tcacttaaca catcaaaggc tttttgctgg
    22441 atttgcgcca gtaagtcata gagctgaatc agtcgctgac catagcgggt cagtactgcg
    22501 ccgccgccac ctttaccgcc tgttgcgcgc tcgaccagaa tatgctcact taactgattc
    22561 atctcgttaa tggcatccca ggcgctttta tagctgatac cggcatcttt cgctccctgg
    22621 ctaatggaac cggaaagcgc aatgtgtttt agtagcgaaa tgcggcgcgg gtcggcgaat
    22681 aatttttgtt ggagcttaag ggtgagaagg atttcggcct gcataacaat gtcctggcaa
    22741 aagtcttatt gtgacggaaa acgaacgcca cgcaaagctg accgcacaaa aggggagtgc
    22801 ttttctgtgc ttagcggtta gaatagtctc atgactatat ctggagttga ccatgttaga
    22861 gttattaaaa agtctggtat tcgccgtaat catggtacct gtcgtgatgg ccatcatcct
    22921 gggtctgatt tacggtcttg gtgaagtatt caacatcttt tctggtgttg gtaaaaaaga
    22981 ccagcccgga caaaatcatt gattccctga atgcccgctt agtcgggcat tttctttttc
    23041 tcaacttcct gcttttcctg ccgatatttt ttcttatcta cctcacaaag gttagcaata
    23101 actgctggga aaattccgag ttagtcgtta tattgtcgcc tacataacgt tacattaagg
    23161 ggttaccaat ggctcgtaaa tggttgaact tgtttgctgg ggcggcactc tctttcgctg
    23221 ttgctggcaa tgcactggca gatgaaggga aaatcacggt gttcgccgcc gcatcactga
    23281 ctaacgcaat gcaggacatt gctacgcagt ataaaaaaga gaaaggcgtg gatgtggttt
    23341 cttctttcgc ttcgtcatct actctcgccc gtcagattga agcgggtgcc cctgcggatc
    23401 tgtttatttc tgccgatcag aaatggatgg attatgcggt tgataaaaaa gcgatcgata
    23461 cagctacgcg tcagacactg ctcggcaata gcctggtcgt tgtagcaccg aaagccagcg
    23521 agcagaaaga ttttaccatc gacagcaaaa ccaactggac ttcactgctg aatggcggtc
    23581 gcctggcggt tggcgatccg gaacatgttc ccgctggcat ttatgcaaaa gaagcactgc
    23641 aaaaactggg cgcatgggat acgctctccc cgaaactggc tccggcggaa gatgttcgtg
    23701 gcgcgctggc gttggtcgaa cgcaatgaag cgccgttggg cattgtttac ggttctgacg
    23761 cagttgctag caaaggggta aaagtggttg ccaccttccc ggaagattca cataaaaaag
    23821 tggaatatcc ggttgctgtt gtggaagggc ataacaatgc gacagtgaaa gcattttatg
    23881 attatctgaa gggaccgcag gcagcggaaa tctttaaacg ttacggattt acaaccaagt
    23941 aatgatactg accgatccag aatggcaggc agttttatta agcctgaaag tttcttccct
    24001 ggctgtgctg tttagcctgc cgtttgggat cttttttgcc tggttactgg tgcgttgcac
    24061 gtttccgggc aaagctctgc tcgacagcgt actgcatcta ccgctggtgt taccgcccgt
    24121 ggtcgtcggt tacttattat tagtttcgat ggggcggcgc ggatttatcg gtgaacgtct
    24181 gtatgactgg tttggtatta ccttcgcctt tagctggcgc ggcgcggttc tcgctgccgc
    24241 cgtcatgtcg tttccgctga tggtgcgggc aattcgtctg gcgctggaag gggttgatgt
    24301 caaactggaa caggccgcaa gaacactggg ggccgggcgc tggcgcgttt tctttactat
    24361 cacgttaccg ctgaccttac cgggaattat tgttggtacg gtactggctt ttgctcgttc
    24421 tctcggtgaa tttggcgcaa ccatcacctt tgtgtcgaac attcctggtg aaacgcgaac
    24481 cattccttct gccatgtata ccctgattca gacccccggc ggggaaagtg gcgcagcgag
    24541 actgtgcatt atttctattg cgctggcgat gatctccctg ttgatttcag aatggctggc
    24601 cagaatcagc cgtgaacggg cggggcgcta atcatgctgg aactgaattt ttcccagacg
    24661 ttgggcaacc attgcctgac tattaatgaa acgctgcccg ctaatggcat cactgctatc
    24721 tttggcgtct ccggtgccgg aaaaacttcg ctgattaacg ccatcagtgg actgacgcgc
    24781 ccgcaaaaag ggaggattgt cctcaatggt cgggtactaa atgatgccga aaaaggtatc
    24841 tgcctgacgc cggaaaagcg tcgcgttggc tatgtttttc aggatgcgcg gctgttcccg
    24901 cattacaaag tgcgtggcaa tctgcgctac ggcatggcga aaagcatggt cgatcagttc
    24961 gataagctgg tggcgctttt aggcattgaa ccgttgcttg accgtttacc aggcagcctg
    25021 tccggaggcg aaaaacagcg cgtagcgatt gggcgggctt tgctgacggc accagaattg
    25081 ctgttgctgg atgaaccgct ggcgtcactg gatattccgc gtaaacgcga actgttgcct
    25141 tatctgcaac gtctgacgcg ggaaatcaac atcccgatgt tgtatgtcag ccattcgctg
    25201 gatgagatcc tccatctggc agacagagtg atggtactgg aaaacggtca ggtgaaagcc
    25261 tttggcgcgc tggaagaagt ctggggtagt agcgtgatga atccgtggct gccgaaagag
    25321 caacaaagta gcattctgaa agtgacggta cttgagcatc atccgcatta cgcgatgacc
    25381 gcgctggcgc tgggcgatca gcatttgtgg gtcaataagc tggacgaacc gctgcaagct
    25441 gcgctacgta tccgcattca ggcttccgat gtttctctgg ttttacaacc gccgcagcaa
    25501 accagcattc gtaacgtatt gcgggcaaaa gttgttaata gttatgacga caacggccag
    25561 gtggaagtgg aactggaagt cggcggtaaa acgctgtggg cgcgtatcag cccgtgggcc
    25621 agggatgaac tggcgatcaa acctggcctg tggctgtacg cgcaaattaa aagtgtgtcg
    25681 ataaccgcct gattaaatca ggtggctata aatgaactgg gcaatgctgt cggtggtgtt
    25741 atcaccaatc acaatgttgg cgcgcgcttt taccgcgtca tcggcgttgc ccatcgccac
    25801 gcctgtacca gcggcctcca gcatactgat atcattaaag ttatcgccga acgccacgac
    25861 gttttccatc gaccagcctt gcgcctcaac ccatttcgtc aaacgtttac ctttgctgtt
    25921 gccgccgcgt gcaatatcaa cctgatcgtg ccaggaccat tcacactcca gtcccagttc
    25981 atgttcgaca tgcttaccaa aatgctgcaa ttgcggcagg tcatcgtgcg tgagggcgaa
    26041 cttccatacg gcgttaactt gttgcgccgt ttcagccaga gaagcgactt gtgtgaaagt
    26101 cggacgctgt tccggcggca gggtttgcgc ccagttagac gtgcgaatga catgcccggt
    26161 cgggtgctca taaaccatct cgtcatcgac atacatcaga ccgtgaatgt ggtgttcatt
    26221 cagcatctca atgagttgta gggctttatt aacgggcatt gggtccgctt ccagcacggt
    26281 ttttgcatga taatcataca aataggtgcc attacagcaa atagcaggtg tatccagcgc
    26341 cagcgcctga taaaaaggat gaatagcgac gtgatggcga cctgtgacga tgattaattg
    26401 ataacctgct tcgcgagcgc gggccagggc ttctatcgat gaaggtagca gggtcttttt
    26461 cggggtcaat aaggtgccgt ctaagtcgag agcaatcacg cgtgtggtca tggcgtattc
    26521 cagattaagg ttaagaattt tcttctgcgc gaatggtaca ccgataccac tatcggacaa
    26581 aattctgcgt tttaattcag cattcaccgc caaaagcgac taattttagc tgttacagtc
    26641 agttgctaaa tgcaaaggag cattcatgaa gcaaacagtt tatatcgcca gccctgagag
    26701 ccagcaaatt cacgtctgga acctgaatca tgaaggcgca ctgacgctga cacaggttgt
    26761 cgatgtgccg gggcaggtgc agccgatggt ggtcagcccg gacaaacgtt atctctatgt
    26821 tggtgttcgc cctgagtttc gcgtcctggc gtatcgtatc gccccggacg atggcgcact
    26881 gacctttgcc gcagagtctg cgctgccggg tagtccgacg catatttcca ccgatcacca
    26941 ggggcagttt gtctttgtag gttcttacaa tgcgggtaac gtgagcgtaa cgcgtctgga
    27001 agatggcctg ccagttggcg tcgtcgatgt ggtcgagggg ctggacggtt gccattccgc
    27061 caatatctca ccggacaacc gtacgctgtg ggttccggca ttaaagcagg atcgcatttg
    27121 cctgtttacg gtcagcgatg atggtcatct ggtggcgcag gaccctgcgg aagtgactac
    27181 cgttgaaggg gccggcccgc gtcatatggt attccatcca aacgaacaat atgcatattg
    27241 cgtcaatgag ttaaacagct cagtggatgt ctgggaactg aaagatccgc acggcaatat
    27301 cgaatgtgtc cagacgctgg atatgatgcc ggaaaacttc tccgacaccc gttgggcggc
    27361 tgatattcat attaccccgg atggtcgtca cttatatgcc tgtgaccgta ccgccagcct
    27421 gattactgtc ttcagcgttt cggaagatgg cagcgtgttg agtaaagagg gcttccagcc
    27481 aactgaaacc cagccgcgcg gctttaatat cgatcaccgt ggtaagtatc tgattgccgc
    27541 cgggcaaaaa tcgcaccaca tctcggtata cgaaattgtt ggtgagcagg ggctactgca
    27601 tgaaaaaggc cgctacgcgg tcgggcaggg accaatgtgg gtggtggtta acgcacacta
    27661 accgctgatt tacccggcgc agtctctcct gcgccggtgt attaacctat ctcctgtaac
    27721 gcatgtctct ggcgttcgac gatattggtc cacaaattgt ctttatcgtc agtccacaaa
    27781 ttaatcagca aggcaaaaaa gcgttctgct gccggagaaa gcacggcatc tttgcggcga
    27841 ataatcccca atgtccgacg tataacgggt tccaccagtg ggataccaat aatagaagaa
    27901 tagggcgcat ggggcattgc cagaccagga agcgccgaaa ttcccagtcc cgcctctacc
    27961 agtcctaatg acgtcgaaag atgacgcact tcgtaaaacc agtccagctt ccagggcttg
    28021 tcggctagct gttgttctat caacaatcgg ttgccactgg aggaacgtac gccaatcatt
    28081 ttgtaaccca ctaactcctg ccattctaca agctgctttt tagccaatgg atggtcacgc
    28141 cgacaggcca gcacgaacgg ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg
    28201 acgttgttca tgtttatgcc aaagtctgat tcgttacaca gcaccgattc catgcaatta
    28261 ttcgtgcctt gttccagaat ccgcacttta atattgggat acagctcatt aaatttaccg
    28321 attgccagcg gtaaaaaata aaatactgcg gtcggaatac acgctaacgt caccatacca
    28381 cgatgataag cattcatatc acgaatatta aaaagcgttt catcgaattt ctttattaat
    28441 tcccgcgcct ctgggagcaa cctttttcct gcttttgtta acgtaacctt gcgagttgta
    28501 cgctcaaata gttgaatatg taaatcttcc tccatctttt ttattcttcg tgttaatgca
    28561 ggttgcgtaa tattgagtaa tttagcggca ttgttaaatg aactggactc tgccagtatg
    28621 acaaatgcct tcatacttga taattcatgc ttcattatta ctccgggaaa tggaagcgac
    28681 gattttgggt ggctggccgt taaaaatttt aactgcattt agccaactta aattaatgaa
    28741 aaaatgttat taatcgttga gctaaagtca ttagagatgc tttgccctta atgtaaccat
    28801 atcgcaataa gttatgtttt taaattgagg gcattattat gaaaaaaata ccctgcgtga
    28861 tgatgcgagg tggaacctcg aggggcgcgt tcctgttagc ggaacattta cccgaagatc
    28921 aaacgcagcg cgataaaata ttgatggcaa ttatgggttc cggtaacgat ctggaaattg
    28981 acggtattgg cggcggtaat ccactgacca gtaaagtcgc cattattagc cgttccagcg
    29041 atccgcgtgc tgatgttgat tatctgtttg ctcaagttat cgtccatgag aaacgtgtcg
    29101 ataccacgcc taactgcggc aatatgctgt ctggcgttgg ggcattcgcc attgaaaatg
    29161 gtttgattgc agcgacttcg ccagttaccc gcgtacgtat ccgcaacgtc aatacgggta
    29221 cgttcatcga agctgatgtg caaacgccaa atggtgttgt cgagtacgag ggtagcgcca
    29281 gaattgacgg cgtaccgggt actgccgcac cggttgcgct cactttcctg aatgccgctg
    29341 gaacgaaaac cggaaaagtt ttcccgactg ataatcagat tgattatttt gacgatgtcc
    29401 cggtgacctg tatcgatatg gcgatgccag tcgtcattat tccggctgaa tatctgggaa
    29461 aaacaggcta tgaattaccg gcggaactgg atgccgacaa agcattatta gcccgcattg
    29521 aatctatccg tctacaagcg ggtaaagcaa tgggcttagg cgatgtcagt aatatggtta
    29581 tccctaaacc tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt
    29641 ttatgccgca ttcttgccat cgcgcgctgg cgataaccgg tgctattgct atttccagta
    29701 gttgtgcatt ggaaggtacc gtcacccgac aaatcgtccc ttctgtagga tacggcaata
    29761 tcaatattga acaccccagt ggtgggctcg acgttcattt aagtaatgaa ggtcaggatg
    29821 ccacgacgtt acgcgcatct gttattcgga caaccagaaa aatattttcc ggtgaagttt
    29881 atcttccctg aaaagattcg ttgtcaggat aaggacaatc aataaaggac ttctgtatga
    29941 gtcatacaga aagaacagga ttttaaatga ataagaaatc gttatggaag ctaattctga
    30001 tattagcgat cccatgtatt attggcttta tgccagctcc ggcaggatta agcgaactgg
    30061 cgtgggtgct ttttggtatt tacctggcgg ccattgtggg gctggttatc aagcctttcc
    30121 cggaacctgt cgtactgtta attgccgttg ctgcctccat ggtggtggtc ggtaacttat
    30181 ccgacggtgc gtttaagacc accgccgtat taagcggtta ctcttcaggt actacctggc
    30241 tggtgttctc ggcgtttacc ttaagcgccg catttgtgac caccggttta ggtaaacgta
    30301 ttgcctatct gctgattggt aaaatcggta acaccacgct gggtctgggt tacgttacgg
    30361 tattcctcga tctggtattg gctccggcaa caccgtctaa caccgcgcgt gcgggcggca
    30421 ttgtgttacc gatcatcaac agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc
    30481 cgcgtcgtgt cggacattac ctgatgatgt ccatttacat ggtcaccaaa accaccagct
    30541 atatgttctt taccgcaatg gcggggaaca ttctggcgct gaaaatgatc aacgacattc
    30601 tgcacctgca aattagctgg ggtggatggg cgctggccgc cggattgccg ggcatcatta
    30661 tgctgctggt caccccgctg gtgatttaca ccatgtatcc accagaaatt aagaaggtgg
    30721 ataacaaaac catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa
    30781 aaatgctgct cggtgtcttt gtgctggcgc tgctgggctg gattttcagt aagtctctgg
    30841 gggttgatga atcttcagtg gctattgttg ttatggcgac catgctgctg ctgggtatcg
    30901 ttacctggga agacgtggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg
    30961 gtattatcgg cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag
    31021 tctttaaaaa taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt
    31081 tcctcagcat catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt
    31141 taccggtatt tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg
    31201 cactgttgtt ctccaactcc tatggcggca tggttactca ctatggcggc gcggcaggtc
    31261 cggtcatctt tggcgtgggt tataacgata ttaaatcctg gtggttggtc ggtgcggtac
    31321 tgacgatatt aaccttcctg gtgcatatca ccctcggcgt gtggtggtgg aatatgctga
    31381 tcggctggaa catgctgtaa atatactcgt catacttcaa gttgcatgtg ctgcgtctgc
    31441 gttcgctcac cccagtcact tacttatgta agctcctggg gattcactcg cttgtcgcct
    31501 tcctgcaact cgaattattt agagtatatc catttatttt cttgctgcgc acttcacggt
    31561 gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag
    31621 taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagcaaa
    31681 aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa
    31741 acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca
    31801 gaccgctaaa gcgtccggta tggaacgttt cccgctgccg tatgtgctga ccaactgcca
    31861 taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc
    31921 ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata
    31981 tatgcgtgag atgatggcag gtggcggcaa aatgatcctc gggtcagaca gtcacacccg
    32041 ttacggtgca ttagggacaa tggcagtcgg tgagggcggc ggcgagttgg taaaacaatt
    32101 gcttaatgac acctgggata tcgactatcc gggagttgtt gcggtgcatc tgaccggaaa
    32161 accagcgccg tatgtggggc cgcaggatgt ggcgttggct atcatcggtg ccgtgttcaa
    32221 aaacggctac gtcaaaaaca aagtgatgga attcgtaggt cccggtgttg ctgcgctctc
    32281 taccgatttc cgtaacagcg ttgacgttat gaccactgaa acgacctgtt taagttctgt
    32341 ctggcaaacc gatgaagaag tccataactg gctggcgctg cacggtcgcg gccaggatta
    32401 ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag
    32461 cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac
    32521 actgaaccag aacttgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc
    32581 gcacggtaaa gccaaactct cgctgctgga caaagtggaa aatggtcgcc tgaaagtgca
    32641 gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa
    32701 tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc
    32761 acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg
    32821 cgcaatcatc agaaccgcgt tctgcggccc atgctttggc gcgggcgata cgccaatcaa
    32881 caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc
    32941 agctaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc
    33001 ggcaaacggt ggctatttaa cctctgccag cgaactcgat tgctgggaca acgtgccgga
    33061 gtacgccttc gatgtaacgc cgtataaaaa tcgcgtctat caaggctttg tgaaaggggc
    33121 ggcccagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct
    33181 gactgacaat atcgtcctca aagtgtgctc gaagatcctc gacgaagtga ccaccaccga
    33241 cgaattgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcgga
    33301 gtttaccctg tcacgccgcg atccaggtta tgttggcagc agtaaagcga ctgctgagct
    33361 ggaaaatcag cgtctggcgg ggaatgtcag cgagctgaca gaggtgtttg cgcgcattaa
    33421 gcagattgct ggtcaggagc atattgatcc gctgcaaact gaaattggca gcatggtcta
    33481 tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg gcgagttgtc agcgtgtgat
    33541 tggcggtctg gcgaatattg ccgaggagta cgcgactaaa cgctatcgtt ctaacgtcat
    33601 caactggggg atgttaccgc tgcagatggt ggaagcgcca acctttgaag tgggggatta
    33661 catttacatc cctggcatta aagcggcgct ggataatccg ggtacgacgt ttaaaggtta
    33721 tgtgatccat gaagatgcgc cggtaacgga aattacgctc tatatggaaa gtctgactgc
    33781 tgaagagcgc gagattatca aggcgggtag tttgattaac ttcaataaaa accgtcagat
    33841 gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt attgtcggat gcgatgctgg
    33901 cgcatcttat ccgacctaca aatcgcatcg aaccgtaggc cggataaggc gtttacgccg
    33961 catccggcaa atagttaata gctcttactt cttcgcctct gcaaccactt tgctacccac
    34021 gccgcggtta ttgtattccc acatgcggtt gtagttagtg tcattcagat tgcgctgtat
    34081 ttcgtcgcta tcgtcaacgc tgccggtgtt acccgcaaac ggacgattag agatcaccgc
    34141 atcgccccac ggtttagccg tgttaaaacc ttcgttgatg gcgctatcac ggatcaccac
    34201 ctgaccgttg gtattggcat caacatccag cgagcggccc agttgtgcca cgccatcacc
    34261 ggaagcattg aaacggctgt ttacggcgag gaaaccgtag taaatgttgg acagcgtagc
    34321 cggtgcaaac acatacgctt cttgctgagt acgtgagttc accacgcgga attcggtgtt
    34381 atcgaacacc actgcgccgc gaccagaaac gatatccaca tccccttcaa tgtagctgtt
    34441 ggtcaccagc gtacgcggct gacgattcgt ttccagacgg ttctgcacac cgctgttggt
    34501 gacaaagaag gtgttctgac gaccgagaat gttaacgttg ttaatctgta cctggtcacc
    34561 gtcagtacgc agtgccaccg ccggatggtt acccgcatct acgctatcac ccagcgtgtt
    34621 ttcgatggtc aggttttgca gttgcaggcc attgttttgt gaccagaaga ccgcagaaca
    34681 cagaacaccg atactgtcgc tgcgtttgct ctggcagcta tcgtacatat accacgctgg
    34741 tttacctggc atatatttgc cgcgcgggtt gacgtcgtga cgccagtcgg cagggctcat
    34801 gcccccatca agggaaagcc caatcttcac atcaatcggt ttttcacctg taccgtacag
    34861 agtaattcca cctggagcag cagggacata caccgttccc tgatactcac caggcatcac
    34921 ggcaatatac tggcgcttgt tggtgcgctt gataattgcc gcatctaccg ccgcctgaat
    34981 cgtggtatgc gttacacctt gagtacccgc cgggccgaca acaaagtcag gttgcgcagg
    35041 cagggttatc ggggacggat tccacgctgc agcacctggt gtcagggatg caaaatagtg
    35101 ttgagcatcg aaattctgcg cttcttttgc cgacagaatc gggcgagaag aggtaccagg
    35161 cgcagtttga tcagaaggac gttgatcggg tggtgttgag ctacaggcgg tcagcgtcac
    35221 gccaaaagcc aatgccagcg ccagacggga aactgaaaat gtgttcacag gttgctccgg
    35281 gctatgaaat agaaaaatga atccgttgaa gtctgctttt ttatactaag ttggcattat
    35341 aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
    35401 cattatttga tttcaatttt gtcccactcc ctgcctctgt catcacgata ctgtgatgcc
    35461 atggtgtccg acttatgccc gagaagatgt tgagcaaact tatcgcttat ctgcttctca
    35521 tagagtcttg cagacaaact gcgcaactcg tgaaaggtag gcggatcccc ttcgaaggaa
    35581 agacctgatg cttttcgtgc gcgcataaaa taccttgata ctgtgccgga tgaaagcggt
    35641 tcacgacgag tagatgcaat tatggtttct ccgccaagaa tctctttgca tttatcaagt
    35701 gtttccttca ttgatatccc gagagcatca acatgcaatg ttgtagggat ggcaattttt
    35761 acgcctgttt tgctttgttc gacataaaga tatccatcta cgatatcaga ccacttcatt
    35821 tcgcataaat caccaactcg ctgcccggta acaacagcca gttccattgc aagtctgagc
    35881 caacatggtg atgattctgc tgcttgataa attttcaggt attcgtcagc cgtaagtctt
    35941 gatctcctta cctctgattt tgctgcgcga gtggcagcga ccgggtttgt tgttatatgg
    36001 ccttcagcta ttgcctctcg gaatgcatcg ctcagtgttg atctgattaa cttggctgac
    36061 gccgccttgc cctcgtctat gtatccattg agcattgccg caatttcttt tgtggtgatg
    36121 tcttcaagtg gagcatcagg cagccccctc cttattgctt taattttgct catgtaattt
    36181 atgagtgtct tctgcttgat tcctctgctg gcgaggattt tttcgtagcg atcaagccat
    36241 gaatgtaacg taacagaatt atcactgttg attctcgctg tcagaggctt gtgtttgtgt
    36301 cctgaaaata actcaatatt ggcctgtatt gcttcagtga ttgctatcct cctgtctcgg
    36361 cctaaaccaa actctttacc cgtccttggg tccctgtagc agtaatattg tttcttatat
    36421 aaaggttagg gggtaaatcc cggcgctcat gacttcgcct tcttcccatt tctgatcctc
    36481 ttcaaaaggc tacctgttac tggtcgattt aagtcaacct ttaccgctga ttcgtggaac
    36541 agatactctc ttccatcctt aaccggagga gggaatatcc tgcattcgcg cacccatcga
    36601 cgaactgttt caaggcttct tgggcgtcgc tggcgtgcgt tccactcctg aagtgtcaag
    36661 tacatcgcaa agtctccgca attacacgca agaaaaagcc gccatcaggc ggcttggtgt
    36721 tctttcagtt cttcaattcg aatattggtt atgtctgcat gtgctatctg cgcccatatc
    36781 atccagtggt catagcagtc attgatgttc tctgcttcga taactctgtt gaatggttct
    36841 ccattccatt cacctgtgac tcggaagtgc atttatcatc tccataaaac aaaacccgcc
    36901 gtagcgagtt cagataaaag aaatcctcgt cagtgcgagg atgctgttca ttgctgctat
    36961 acactttttt gctctcaacg taagcggtag ctcattctgt tgggttggtg cagttgcttt
    37021 taggaaatgc tatttacccc ttaaacgtcg gctgaaagag ctaaaatcca tgcaaaaaat
    37081 ttacgcaatt ttgtgtatta ttgtgcagta agtaatgagc tattttctgc gcaaaaaatg
    37141 gatggtaaat ttgtccgggg caggaaaaat tttatgggcg ctaaacatga aaaaagattc
    37201 gtatccttat ttgatttgca tgacagtttt aggcctgatc tttattttcc ttttcttctg
    37261 gtggcgggca gatatttaca gggtcacgtt tcttaatcag agtatatccc actattacat
    37321 tctgtttagc atgggaatag cttttctgtt atctctgttt tgggttaaga aggggatagt
    37381 aaaacaaagc ggctggaaga gtctgtcagc ataccttaag gtttatgcag ggatgtgcat
    37441 atttgctgga ttttttctga ttatacccct tacaacacta acttattttt tgcctggaga
    37501 gacatcgtct tatgttgcac cgtatcggta tacttccggt agttcaaaaa gttgttctgg
    37561 agctgaggtg gatgaccccg atctacatga gaatattcgc atttgctatc cgtatggcaa
    37621 ttatgagtac gataatatta tctatgttga aaagaaaatt aatacattag gtgcggtagt
    37681 aacatatgca cagaccgcgc gtgatgatac tgaatgagat agtatatagc gggcaagttt
    37741 tagttaattt atcgaggtga tataatttac ctcgactcgt ttattctggt attaatattt
    37801 cgctttacga tcgattttta tctgatgata tcatgcggtt ttcatatact gacttactgt
    37861 cttttctccg ttagcgattt tctcctgctc agcgatgatt ttatctttgg cttctagtta
    37921 atttcgctca cttcgaacct ctctgtttac tgataagttc cagatcctcc tggcaacttg
    37981 cacaagtccg acaaccctga acggccaggc gtcttcgctc atctatggga tcgccacact
    38041 cacaacaatg agtggcagat atagcctggt ggttcaggcg gcgcattttt attgctgtgt
    38101 tgcgctgtaa ttcttcaatt tctgatgctg aatcaatgat gtctgccatc ttccattaat
    38161 ccctgaattg ttggttaata cgcttgaggg tgaatgcgaa taataaaaaa ggagcctgta
    38221 gctccctgat gattttgctt ttcatgttca ccgttcctta aagacgccgt ttaacatgcc
    38281 gattgccaga cttaagtgag tcggtgtgaa tcccatcagc gttaccgttt cgcggtgctt
    38341 cttcagtacg ctacggcaaa tgtcatcgac gtttttatcc ggaaacggct gtctggcttt
    38401 tttgatttca gaattagcct gacgggcaat actgcgaagg gcgttttctt gctgaggtgt
    38461 cattgaacaa gtcccatgtc ggcaagcata agcacacaga atatgaagcc cgctgccaga
    38521 aaaatgcatt cagtggttgt cataccaggt ctctctcatc tgcttctgct ttcgccacca
    38581 tcatttccag cttttgcgaa agggatgtgg ctaacgtatg aaattcttcg tctgtttcta
    38641 ctggtattgg cacaaacctg actccaattt gagcaaggct atgtgccatc tcaatactcg
    38701 ttcttaactc aacaggagat gctttgtgca tatcgcctcc cgtttattat ttatctcctc
    38761 agccagccgc tgggctttca gcggatttcg gataacagaa aggccgggaa atacccagcc
    38821 tcgcttcgta acggagtaga cgaaagtgat cgtgcctacg cggatattat cgtgaggatg
    38881 cttcatcgcc attgctcccc aaatacaaaa ccaatttcag ccagtgcctc gtccattttt
    38941 tcgatgaact ccggcaccat ctcgtcaaaa cccgccatgt acttttcatc ccgctcaacc
    39001 acgacataat gcaggccttc acgcttcata cgcgggtcat agttggcaaa gtaccaggca
    39061 tcttttcgcg tcacccacat gctgtactgc acctgggcca tgtaagccga ctttatggcc
    39121 tcgaaaccac cgagcctgaa cttcatgaaa tcccgggagg taaacgggca tttcagctca
    39181 aggccgttgc cgtcactgca taaaccatcg ggagagcagg cggtgcgcat actttcgtcg
    39241 cgatagatga tcggggattc agtaacattc acgccggaag tgaattcaaa cagggttctg
    39301 gcgtcgttct cgtactgttt tccccaggcc agcgccttag cattaacttc cggagccaca
    39361 ccggtgcaaa cctcagccag cagggtgtgg aagtaggaca ttttcatgtc aggccacttc
    39421 tttcctgagc ggggctttgc tatcacgttg tgaacttctg aagcggtgat gacgccgagc
    39481 cgtaatttgt gccatgcatc atccccctgt tcgacagctc tcacgtcgat cccggtacgc
    39541 tgcaggataa tgtccggtgt catgctgcca ccttctgctc agtggctttc tgtttcagga
    39601 atccaagagc tttcactgct tcggcctgtg tcagttctga cgatgcgcga atgtcgcggc
    39661 gaaatatctg ggaacagagc ggcaataagt cgtcatccca tgttttatcc agggcgatta
    39721 gcagagtgtt aatctcctgc atggtttcat cgttaaccgg agtgatgtcg cgttccggct
    39781 ggcgttctgc agtgtatgca gtattttcga caatgcgctc ggcttcatcc ttgtcataga
    39841 taccagcaaa tccgaaggcc agacgggcac actgaatcat agctttatgc cgtaacatcc
    39901 gtttgggatg cgactgccac ggccccgtga tttctctgcc ttcgcgggtt ttgaatggtt
    39961 cgcggcggca ttcatccatc cattcggtaa cgcagatcgg atgattacgg tccttgcggt
    40021 aaatccggca tgtacaggat tcattgtcct gctcaaagtc catgccatca aactgctggt
    40081 tttcattgat gatgcgggac cagccatcaa cgcccaccac cggaacgatg ccgttctgct
    40141 tatcagggaa ggcgtaaatt tctttcgtcc acggattaag gccgtactgg ttggcgacga
    40201 tcaacaatgc gatgaactgc gcatcgctgg catgaaagcg cgatggggag acagggcggc
    40261 atgagacgac agcgacgaag tttcaccgac atcatctgcg aaaactgcaa ataccttcca
    40321 acgaaacgct ccagaaataa acgcaagcca atcccaaaag aatctgacgt aaaaaccttc
    40381 aactacacgg ctcacctgtg ggatatccgg tggctaagac attgtgcgag gaaaacaagg
    40441 tgattgacca aaatcgaagt tacgaacaag aaagcgtcga gcgggcttta acgtgcgcta
    40501 actgcggtca gaagctgcat gtgctggaag ttcacgtgtg tgagcactgc tgcgcagagc
    40561 tgatgagcga ttcgaatagc tcgatgcacg aggaaaaaga tgatggctaa accagcgcga
    40621 agacgatgta aaaacgatga atgtcgggaa tggtttcacc ctgcattcgc taatcagtgg
    40681 tggtgctctc cagagtgtgg aaccaagata gcactcgaac gacgaagcaa agaacgcgaa
    40741 aaagcggaaa aggcagaaaa ggcagcagag aagaaacgac gacgagagga gcagaaacag
    40801 aaagataaac ttaagattca aaaactcgcc ttaaagcccc gcagttactg gattaaacaa
    40861 gcccaacaag ccgtacacgc cttcatcaga gaaagagacc gcgacttacc atgtatctcg
    40921 tgcggaacgc tcacgtctgc tcagtgggat gccggacatt accggacaac tgctgcggca
    40981 cctcaactcc gatttgatga acgcaatatt cacaagcaat gcgtggtgtg caaccagcac
    41041 aaaagcggaa atctcgttcc gtatcgcgtc gaactgatta gccgcatcgg gcaggaagca
    41101 gtagaggaaa tcgaatcaaa ccataaccgc tatcgctgga ctgtcgaaga gtgcagggcc
    41161 atcaaggcgg agtatcaaca gaaacttaaa aaactgcgaa acagcagaag tgaggttgca
    41221 tgaatatcta cgaaagaatt gatggcagca aataccgaaa tatttgggta gttggcgatc
    41281 tgcacggatg ctacacgaac ctgatgaaaa aactggagac gataggattc gacaccaaaa
    41341 aagacctgct tatctcggtg ggcgatttgg ttgatcgcgg tacagagaac gtagaatgcc
    41401 tggaattaat cacattcccc tggttcagag ctgtacgtgg aaaccatgag caaatgatga
    41461 ttgatggctt atcagagcgt ggaaacgtca atcactggct gcttaatggc ggtggctggt
    41521 tctttaatct cgattacgac aaagaaattc tggctaaagc tcttgcccat aaagcagatg
    41581 aacttccgtt aatcatcgaa ctggtgagta aaggaaaaaa atatgtcatc tgccacgccg
    41641 attatccttg tgataaatac gagtttggaa agccagttga tcatcagcag gtaatctgga
    41701 accgcgaacg aatcagcaac tcacaagacg ggatcgtgaa agaaatcaaa ggcgcggaca
    41761 cgttcatctt tggtcatacg ccagcagtga aaccactcaa atttgccaac cagatgtata
    41821 tcgatactgg cgcagtgttc tgcggaaacc tcacattgat tcaggtacag ggagaaggcg
    41881 catgagactc gaaagcgtag ctaaatttca ttcgccaaaa agcccgatga tgagcgactc
    41941 accacgggcc acggcttctg actctctttc cggtactgat gtgatggctg ctatggggat
    42001 ggcgcaatca caagccggat tcggaatggc tgtattctgt ggtaagcacg aactcagcca
    42061 gaacgacaaa caaaaggcta tcaactatct gatgcaattt gcacacaagg tatcggggaa
    42121 ataccgtggc gtggcaaagc ttgaaggaaa tactaaggca aaggtactgc aagtgctcgc
    42181 aacattcgct tatgcggatt attgccgtag tgccgctacg ccgggcgcaa gatgcagaga
    42241 ttgccacggt acaggccgtg cggttgatat agccaaaaca gagcagtggg ggatagttgc
    42301 tgagaaagag tgcggaagat gtaaaggcgt cggttattca agaatgccag caagcgccgc
    42361 atatcgcgct gtgacgatgc taatcccaaa ccttacccaa cccacctggt cacgcactgt
    42421 taagccgctg tatgacgctc tggttgtgca atgccacaag gaagagtcaa tcgcagacaa
    42481 cattttgaat gcgatcacac gttagcgcca tgattgccac ggatggcaac atattaacgg
    42541 cataatattg actttttgaa taactttggg gaaacttgac accaataatg ggcgtttttt
    42601 acatgtcatt gatgagtctc aataacctgc cgccgagtag tttttatgct ctgaattgta
    42661 tttgtgtagt aaacatgctg actgcaatgt aatagagttt ttttagcctg taacctcttg
    42721 acggcattga attgcttttg ttatgagttg taagccaatg ttatcatctt gtattggggt
    42781 ggttatgaag gatggtgcgc tgctcaggag ttcttcactt tttattgcct acatgggatg
    42841 ccttggatgg gggagtgctt atttctatgg atggggtact tctttttact acggcttccc
    42901 atggtggatt gtaggtgcag gtgttgatga tgttgccaga agtttatttt ttgcagttat
    42961 cgtcattgct atatttctta tcggttgggg tattggtgtt gtattctttt tcgcagtgaa
    43021 aagaaaacat tctatgcaag agctaaatgt atttcgcctt tattttgctg tggaattatt
    43081 gtttgtgccg gcaattattg agttttctat attgagacag aagattcagg tacctctttt
    43141 gctactgtca gcagcgattg cgctggcggt cacaatttcg ataagatctt atgggcgatt
    43201 tttatcggta tcatgcttct atgataagcc atttataaaa aaacattttt ttgagattgt
    43261 gatgattgct tttgtggcat atttctggct tttttcattt ctgacaggat attacaaacc
    43321 gcagtttaag aaagaatatg aaatgattaa ttataatgat ggttggtatt atgttcttgc
    43381 tcgttatgat aattgtctgg ttttgtctac ttctttcaa
//
LOCUS       DAZKIF010000022        43312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZKIF010000022 DAZKIF010000000
VERSION     DAZKIF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.022"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(152..898)
                     /gene="cutC"
                     /locus_tag="ACHOTW_001266"
     CDS             complement(152..898)
                     /gene="cutC"
                     /locus_tag="ACHOTW_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9843410.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(912..1478)
                     /gene="yecM"
                     /locus_tag="ACHOTW_001267"
     CDS             complement(912..1478)
                     /gene="yecM"
                     /locus_tag="ACHOTW_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9843411.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1694..3427
                     /gene="argS"
                     /locus_tag="ACHOTW_001268"
     CDS             1694..3427
                     /gene="argS"
                     /locus_tag="ACHOTW_001268"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9843412.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3469..3513)
                     /locus_tag="ACHOTW_001269"
     CDS             complement(3469..3513)
                     /locus_tag="ACHOTW_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843413.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            3604..4092
                     /gene="yecT"
                     /locus_tag="ACHOTW_001270"
     CDS             3604..4092
                     /gene="yecT"
                     /locus_tag="ACHOTW_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFP9843414.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4212..4604)
                     /gene="flhe"
                     /locus_tag="ACHOTW_001271"
     CDS             complement(4212..4604)
                     /gene="flhe"
                     /locus_tag="ACHOTW_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP9843415.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(4604..6682)
                     /gene="flhA"
                     /locus_tag="ACHOTW_001272"
     CDS             complement(4604..6682)
                     /gene="flhA"
                     /locus_tag="ACHOTW_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP9843416.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6675..7823)
                     /gene="flhB"
                     /locus_tag="ACHOTW_001273"
     CDS             complement(6675..7823)
                     /gene="flhB"
                     /locus_tag="ACHOTW_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP9843417.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8025..8669)
                     /gene="cheZ"
                     /locus_tag="ACHOTW_001274"
     CDS             complement(8025..8669)
                     /gene="cheZ"
                     /locus_tag="ACHOTW_001274"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP9843418.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8680..9069)
                     /gene="cheY"
                     /locus_tag="ACHOTW_001275"
     CDS             complement(8680..9069)
                     /gene="cheY"
                     /locus_tag="ACHOTW_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP9843419.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9084..10133)
                     /gene="cheB"
                     /locus_tag="ACHOTW_001276"
     CDS             complement(9084..10133)
                     /gene="cheB"
                     /locus_tag="ACHOTW_001276"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP9843420.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10136..10996)
                     /gene="cheR"
                     /locus_tag="ACHOTW_001277"
     CDS             complement(10136..10996)
                     /gene="cheR"
                     /locus_tag="ACHOTW_001277"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP9843421.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11015..12616)
                     /gene="tap"
                     /locus_tag="ACHOTW_001278"
     CDS             complement(11015..12616)
                     /gene="tap"
                     /locus_tag="ACHOTW_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP9843422.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNTSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12662..14323)
                     /gene="tar"
                     /locus_tag="ACHOTW_001279"
     CDS             complement(12662..14323)
                     /gene="tar"
                     /locus_tag="ACHOTW_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP9843423.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14466..14969)
                     /gene="cheW"
                     /locus_tag="ACHOTW_001280"
     CDS             complement(14466..14969)
                     /gene="cheW"
                     /locus_tag="ACHOTW_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP9843424.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14990..16954)
                     /gene="cheA"
                     /locus_tag="ACHOTW_001281"
     CDS             complement(14990..16954)
                     /gene="cheA"
                     /locus_tag="ACHOTW_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP9843425.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16959..17885)
                     /gene="motB"
                     /locus_tag="ACHOTW_001282"
     CDS             complement(16959..17885)
                     /gene="motB"
                     /locus_tag="ACHOTW_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP9843426.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17882..18769)
                     /gene="motA"
                     /locus_tag="ACHOTW_001283"
     CDS             complement(17882..18769)
                     /gene="motA"
                     /locus_tag="ACHOTW_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP9843427.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18896..19474)
                     /gene="flhC"
                     /locus_tag="ACHOTW_001284"
     CDS             complement(18896..19474)
                     /gene="flhC"
                     /locus_tag="ACHOTW_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP9843428.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19477..19827)
                     /gene="flhD"
                     /locus_tag="ACHOTW_001285"
     CDS             complement(19477..19827)
                     /gene="flhD"
                     /locus_tag="ACHOTW_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP9843429.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            20607..21035
                     /gene="uspC"
                     /locus_tag="ACHOTW_001286"
     CDS             20607..21035
                     /gene="uspC"
                     /locus_tag="ACHOTW_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP9843430.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(21042..22466)
                     /gene="otsA"
                     /locus_tag="ACHOTW_001287"
     CDS             complement(21042..22466)
                     /gene="otsA"
                     /locus_tag="ACHOTW_001287"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP9843431.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGLSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAVALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLV"
     gene            complement(22441..23241)
                     /gene="otsB"
                     /locus_tag="ACHOTW_001288"
     CDS             complement(22441..23241)
                     /gene="otsB"
                     /locus_tag="ACHOTW_001288"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP9843432.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(23408..24394)
                     /gene="araH"
                     /locus_tag="ACHOTW_001289"
                     /pseudo
     CDS             complement(23408..24394)
                     /gene="araH"
                     /locus_tag="ACHOTW_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
     gene            complement(24409..25923)
                     /gene="araG"
                     /locus_tag="ACHOTW_001290"
     CDS             complement(24409..25923)
                     /gene="araG"
                     /locus_tag="ACHOTW_001290"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP9843433.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(25993..26982)
                     /gene="araF"
                     /locus_tag="ACHOTW_001291"
     CDS             complement(25993..26982)
                     /gene="araF"
                     /locus_tag="ACHOTW_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP9843434.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            27779..28282
                     /gene="ftnB"
                     /locus_tag="ACHOTW_001292"
     CDS             27779..28282
                     /gene="ftnB"
                     /locus_tag="ACHOTW_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP9843435.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(28362..28613)
                     /gene="yecJ"
                     /locus_tag="ACHOTW_001293"
     CDS             complement(28362..28613)
                     /gene="yecJ"
                     /locus_tag="ACHOTW_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP9843436.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(28728..28814)
                     /gene="azuC"
                     /locus_tag="ACHOTW_001294"
     CDS             complement(28728..28814)
                     /gene="azuC"
                     /locus_tag="ACHOTW_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP9843437.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            29076..29399
                     /gene="yecR"
                     /locus_tag="ACHOTW_001295"
     CDS             29076..29399
                     /gene="yecR"
                     /locus_tag="ACHOTW_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP9843438.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGISDRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            29570..30067
                     /gene="ftnA"
                     /locus_tag="ACHOTW_001296"
     CDS             29570..30067
                     /gene="ftnA"
                     /locus_tag="ACHOTW_001296"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP9843439.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(30104..30343)
                     /gene="yecH"
                     /locus_tag="ACHOTW_001297"
     CDS             complement(30104..30343)
                     /gene="yecH"
                     /locus_tag="ACHOTW_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP9843440.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            30535..31746
                     /gene="tyrP"
                     /locus_tag="ACHOTW_001298"
     CDS             30535..31746
                     /gene="tyrP"
                     /locus_tag="ACHOTW_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP9843441.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(31808..32473)
                     /gene="yecA"
                     /locus_tag="ACHOTW_001299"
     CDS             complement(31808..32473)
                     /gene="yecA"
                     /locus_tag="ACHOTW_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP9843442.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(32830..33831)
                     /locus_tag="ACHOTW_001300"
     CDS             complement(32830..33831)
                     /locus_tag="ACHOTW_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9843443.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(33837..34184)
                     /locus_tag="ACHOTW_001301"
     CDS             complement(33837..34184)
                     /locus_tag="ACHOTW_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFP9843444.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(34214..34864)
                     /locus_tag="ACHOTW_001302"
     CDS             complement(34214..34864)
                     /locus_tag="ACHOTW_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843445.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHIESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(34880..35284)
                     /locus_tag="ACHOTW_001303"
     CDS             complement(34880..35284)
                     /locus_tag="ACHOTW_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9843446.1"
                     /translation="MKNDIGRRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(35374..35511)
                     /locus_tag="ACHOTW_001304"
     CDS             complement(35374..35511)
                     /locus_tag="ACHOTW_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843447.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            35583..35786
                     /locus_tag="ACHOTW_001305"
     CDS             35583..35786
                     /locus_tag="ACHOTW_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9843448.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            35808..36158
                     /locus_tag="ACHOTW_001306"
     CDS             35808..36158
                     /locus_tag="ACHOTW_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP9843449.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            36169..36447
                     /locus_tag="ACHOTW_001307"
     CDS             36169..36447
                     /locus_tag="ACHOTW_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843450.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGIVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            36459..36701
                     /locus_tag="ACHOTW_001308"
     CDS             36459..36701
                     /locus_tag="ACHOTW_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP9843451.1"
                     /translation="MGKEYKTLINKAPERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            36698..36811
                     /locus_tag="ACHOTW_001309"
     CDS             36698..36811
                     /locus_tag="ACHOTW_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843452.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDK"
     gene            36904..37320
                     /locus_tag="ACHOTW_001310"
     CDS             36904..37320
                     /locus_tag="ACHOTW_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843453.1"
                     /translation="MNAKEKNIINTLKIVSAEQDKLSRAAQKDNQHMAALYALTIAIA
                     TPEAAKVIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMLQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            37344..37547
                     /locus_tag="ACHOTW_001311"
     CDS             37344..37547
                     /locus_tag="ACHOTW_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843454.1"
                     /translation="MAIKHFPVVRFTSRGREYEVNERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            37544..37810
                     /locus_tag="ACHOTW_001312"
     CDS             37544..37810
                     /locus_tag="ACHOTW_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9843455.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            37807..38106
                     /locus_tag="ACHOTW_001313"
     CDS             37807..38106
                     /locus_tag="ACHOTW_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843456.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDNVFDFFLNQ"
     gene            38429..38659
                     /locus_tag="ACHOTW_001314"
     CDS             38429..38659
                     /locus_tag="ACHOTW_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP9843457.1"
                     /translation="MADRKQHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHEFQQLIQQQNKLH"
     gene            38732..39097
                     /locus_tag="ACHOTW_001315"
     CDS             38732..39097
                     /locus_tag="ACHOTW_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843458.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATACRDTLNGMTK"
     gene            39104..41926
                     /locus_tag="ACHOTW_001316"
     CDS             39104..41926
                     /locus_tag="ACHOTW_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9843459.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRVENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRQKGKKSWQAERYARGNLRKIFERLERTDHRWLTQGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMTESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFTTYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPGVEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRRDI"
     gene            42003..42962
                     /locus_tag="ACHOTW_001317"
     CDS             42003..42962
                     /locus_tag="ACHOTW_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP9843460.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKAPADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVATAIREACGVNATFIADGVPQFALVNGLYAMNKE"
     gene            42967..43281
                     /locus_tag="ACHOTW_001318"
     CDS             42967..43281
                     /locus_tag="ACHOTW_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP9843461.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQANQPSGVECNFYKIKKNIQTLF
                     PE"
BASE COUNT        10999 a        11516 c        10211 g        10586 t
ORIGIN      
        1 cacaacgcta tgtgcactgt cacccgctca acagatcgcg gtaatcgttc attttttata
       61 ctattcaggg ataaaaaaag gcccctgttg aaattgcagg ggcctggtac gagcaagcat
      121 catattgggc gacatgatgc aacggtaaaa atcatttggc ctgatggcgt tcaatgattc
      181 ctttcatttc agcaaccgcc gccccgtcta cgacataacg cgaatactcg tccgcgtgtg
      241 catctgatga catggacaat ccttgattac gataacgcat cggtgaggct tgccacgctc
      301 ccgcggagct atggacttcc agcactccgg catcgaggaa gtggtgcagg ttttctgcac
      361 ggactcctgc tccggccata atgattggag catcacgatg ggcaataagt tccataattt
      421 ttgataaacc ttgcagtgcg tctgattttt gccctgatgt cagtacccgg gcaatgccca
      481 attccgcaag attattgagt gtatttagag ggttagcgca catatcaaag gcgcgatgaa
      541 aagtcactgc cagcggaccg gcagcagcca ttattttttc cattcgtgac atatcgacat
      601 taccgtcaac atcgagcacg cccgtcacga gtccaggaaa acctaattcg cggaccgtgc
      661 gcacatcctc aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg
      721 ggcgaattat cggatgcaca gggatcgtca cccgctggcg cacggatttc agtacaccca
      781 acgacggcgt taagcccccc tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt
      841 tttgctgcgc cgttagcgcg cattccatgc tgtaacagca aatttccagt aatgccattt
      901 ttgctcctta attacgccga ctgctcgctg gcaacaatct cttcaatgga ccacggatga
      961 aatttaatgg tcgttttgcc atcggtgacc gccagcgtcg gattcggtaa tcgttcatgc
     1021 tcaccttttg gcgaactggt tttaactgag atccccggta ggctgagtcc ttcatcagaa
     1081 agcaatgcca gcgcacgggc attcagcgtt tctggatcac ccggcaaaac gatttcaata
     1141 tgttcccaac cttcgtgcgg gtaacgtttt tccctaggcc acggtagctc cacaatagaa
     1201 aactgccagt gtgcaacctg taccggttca tgcaatttaa acagacaaat cggtctgcca
     1261 ttgatcatat tttctgacaa aagctcgcca cactgttcaa acccgcgacg ccagcgctca
     1321 gcagtggcgt tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg agtgatattc
     1381 aaccccagac ggcgggaaag ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca
     1441 atatcctgca gctcgtcgat agattgccag ttcgccataa tcactcttcg tctttcagta
     1501 aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg ctgatttcac gccgcttctg
     1561 atgcaatagt gaaaacggca atacgccacg cgcacgttgc tgacgaaaac agccatttgc
     1621 agtatactcc cgccctaatt tctttaactg gtgcgggcaa tttttgctcg cttcatcaat
     1681 gtaaggtatt ccggtgaata ttcaggctct tctctcagaa aaagtccgtc aggccatgat
     1741 tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt
     1801 cggcgactat caggctaacg gcatgatggc agttgctaaa aaactgggta tggcaccgcg
     1861 acaattagca gagcaggtgc tgactcatct ggatcttaac ggtatcgcca gcaaagttga
     1921 gatcgccggt ccaggcttta tcaacatttt ccttgatccg gcattcctgg ctgaacatgt
     1981 tcagcaggcg ctggcgtccg atcgtctcgg tgttgctacg ccagaaaaac agaccattgt
     2041 ggttgactac tctgcgccaa acgtggcgaa agagatgcac gtcggtcacc tgcgctctac
     2101 cattattggt gacgcagcag tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc
     2161 aaaccacgtc ggcgactggg gcactcagtt cggtatgctg attgcatggc tggaaaagca
     2221 gcagcaggaa aacgccggtg aaatggagct ggctgacctt gaaggtttct accgcgatgc
     2281 gaaaaagcat tacgacgaag atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa
     2341 actgcaaagc ggtgacgaat atttccgcga gatgtggcgc aaactggtcg acatcaccat
     2401 gacgcagaac cagatcactt acgatcgtct caacgtgacg ctgacccatg atgacgtgat
     2461 gggcgaaagc ctctacaacc caatgctgcc aggaattgtg gcggatctca aagccaaagg
     2521 tctggcagta gaaagcgaag gggcgaccgt cgtattcctt gatgagttta aaaacaagga
     2581 aggcgaaccg atgggcgtga tcattcagaa gaaagatggc ggctatctct acaccaccac
     2641 tgatatcgcc tgtgcgaaat atcgttatga aacactgcat gccgatcgcg tgctgtatta
     2701 catcgactcc cgtcagcatc aacacctgat gcaggcatgg gctatcgtcc gtaaagcagg
     2761 ctatgtaccg gaatccgtac cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga
     2821 cggcaaaccg ttcaaaaccc gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga
     2881 agccctggaa cgtgcacgcc gtctggtggc agaaaagaac ccggatatgc cagccgacga
     2941 gctggaaaaa ctggctaacg cggttggtat tggtgcggtg aaatatgcgg atctctccaa
     3001 aaaccgcacc acggactaca tcttcgactg ggacaacatg ctggcgtttg agggtaatac
     3061 cgcgccatac atgcagtatg catacacgcg tgtattgtcc gtgttccgta aagcagaaat
     3121 tgacgaagag caactggctg cagctccggt tatcatccgt gaagatcgtg aagcgcaact
     3181 ggcagctcgc ctgctgcagt ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc
     3241 gcatgtaatg tgtgcttacc tgtacgatct ggccggtctg ttctctggct tctacgagca
     3301 ctgcccgatc ctcagcgcag aaaacgaaga agtgcgtaac agccgtctga aactggcaca
     3361 actgacagcg aagacgctga agctgggtct ggatacgctg ggtattgaga ctgtagagcg
     3421 tatgtaatcg atttttcgtg agagtgaagc ctgatcaggt ttagccgatc aggctttttt
     3481 attaccatct aaatgtattc tgaaaatagg catgccattg ttttctcgct gttgagtaag
     3541 aggccagaag cgtaatatcc agctccaggg aaacggtaac ggttcaattt aaggaatacc
     3601 gcagtgttta aatttcttgt attaacatta ggcattatct cttgccaggt ttacgcagaa
     3661 gatacgctta tagtaaatga tcatgacatt tcagccatca aagattgttg gcaaaaaaat
     3721 tcaggtgatg atactgacat taacgtgatc aaatcatgtc tgcgacaaga atacaatctc
     3781 gtcgatgcgc aattaaataa agcttatggt gaagcttatc gttatataga acaagtgcca
     3841 cgcacaggtg caaaaaaacc tgatactgaa caacttaact tgcttaaaaa atcacaacga
     3901 gcctggctgg attttaggga caaagaatgt gaattaatcc tttcaaatga ggacgttcag
     3961 gatttaagta acccttattc tgaatcagaa tggctatcat gtatgatcat acagaccaat
     4021 acgcgaactc gccagttgca gctataccgt aactctgaag atttttatcc aagccctttg
     4081 acaagaggat aattcacata tttttggcat gttttgttgc aagctattcc tgataaatga
     4141 ttgcaacaaa acatccagcc tttttcactg agttattaaa catactcgcg agcgcgtaat
     4201 ttttttgtcc ttcagcgata attcacaatc acttcattac gttgtacttt cagcgacgga
     4261 attaaccgcc caccgccagg cacttcccag ataaagcgca acggttctgc tgccgctata
     4321 ccggaaaagg ccacggtggt tccgctctgc ccatctaatt cgacacaacg agattgcgaa
     4381 cacaagcgaa cccgcagtcc tgcaggtgtc gggccgataa gctgataccg ccacgccacc
     4441 agcgtcatca atcctgaagc aggttgtcgc gtagaaagag gtgcagacga catcgactca
     4501 ccgcgatgat ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct
     4561 tgcaccagca gcggaaacaa taatattgct aataaggttc tcatcattta ccgccaattg
     4621 tcgccgtcat gcggatatgt cggttatcag acagttccag attcgacagc accactaact
     4681 gcggcaagct gcggcgcaga aagcgagaca ataatggtcg cagcgcgtgg ttcaccaata
     4741 aaactggcgg cgcacccaac atctcctgac gggatagcgc ttcctgagtt tgcgccagta
     4801 aacgatccgc cagccctggc tccagtccgc ccccgccctg caacgcctgt agcaacaaac
     4861 gttccagcgg tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct
     4921 gggtaatcgc ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt
     4981 ggatgggcgc atgttccgcc agcgtttcga gaatagtgcg catatcgcga atcggtactt
     5041 tttcatcgag gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggga
     5101 cgagatcttc cgtcagcttt ggcatctcct gggcgacgcg atccagcagc tgttgcgcct
     5161 cctgacgacc aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta
     5221 ccaccgtgct ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac
     5281 tttcaatcca gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg
     5341 tcccggcagc ggttccaggg ttaatagcca gccagcgccc cggataagca tcaccactgc
     5401 ctatctccac gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc
     5461 gaatgtgcac cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc
     5521 gtatacggcc caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac
     5581 ccacttccat tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg
     5641 cattattctc tgccattttt accggttttg gttcggcagg cgctttttgt tcgcgtccac
     5701 ggatccacca ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca
     5761 ttccaggcac caggccgagt aaaccgagca cggcagcgct taacaacata acgcttgggt
     5821 tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa
     5881 caatgacccc cgcggcggta gaaatcacca gagccgggat ttgtgccacc agaccgtcac
     5941 caatggtcaa gagcgtataa ctttccgccg cgtgtcccat gctcatgcca tgttgcagca
     6001 cgccgaccag caacccgccg acaacgttaa tgaccatgat gaggatcccg gcgatggcat
     6061 cgccgcgaac aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtga
     6121 cttcggagcg gcgttttttc gcctcatctt caccaatcaa tccggcgtta aggtcggcgt
     6181 caatcgccat ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcga
     6241 tacgcccggc acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca
     6301 cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaacgcttca accaccttcc
     6361 ctgccgccgc tgcgccggta tgcccttcca ttaaaatgat acgggttgaa gccacgttaa
     6421 gtgccagacg caacagcgtg gtaaacaaca aaatggtcgg aaacgcagca aactcaagcg
     6481 tgcgctgggt aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa
     6541 acaacaggtc gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga
     6601 tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt caggtttgtg ggcaggcgca
     6661 gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc
     6721 accggaagat gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt
     6781 tgccagaccc aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga
     6841 ccaatctcca catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg
     6901 ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc
     6961 actttcggtg cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg
     7021 ttattgacaa tgacatccgc tttcggcaca tcggccatca tccgacgccg tgcggcagct
     7081 cgctgcatct gacggatccg ccctttaaca tgggggtcgc cttcgctttg tttgaactca
     7141 tcacgaatat cctgccgcga cattcgcagc tttttcaggt ggctgaagat ttggaaaaag
     7201 acgtcaaatc ccaccattgg aatgacacca agcaccacca gcagtgcgca tagccctacc
     7261 aaatccatcg cattacccat ggcggtaatc ggagactcgg ccattaagcg catcatatgc
     7321 ggccagtgat gccagagaaa aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt
     7381 gctttaagca actccgcgcc agtctgtgcc gagaacatcc gttttatgcc cggtagcggg
     7441 ttgagtttgg aaaacttcgg ctgcaaggat tttccgctaa ataccagccc ccccagcata
     7501 accggagaaa tgagcgccac cagcaccacg ccgctaatca gcggtagcag cgccagcatg
     7561 gcttctctga tcagcagaat aatttgcccg aggatcagat tcgggtcttt gataatactg
     7621 tgatcaaaat gcagcccagc ggagagcatg cccgacaatc gacgggccag cgacacaccg
     7681 ccaaaccaga taacactaac gcccaccagc aaaatcagca gtgaggtcag ttcacgggaa
     7741 cgcgggattt gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg ggcttctgtt
     7801 ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa gagtattaag cgtaaatgat
     7861 gccagagcgc aaagcgttca atggtttgag taaggggcaa aacaggcggg atttagggct
     7921 tttgctgcca cacatcaagc atagtgtgcg tttgtcggat gcggcgtcat cgccttatca
     7981 gaccgcctga tatgacgtgg tcacgccaca tcaggcaata caaatcaaaa tccaagacta
     8041 tccaacaaat cgtccacctg atcctgactg gctaccacac cggctttgct ggtatcgacc
     8101 tgaggtccat taagcaaact ctggttttca cgttttggac gcgactcctg ttccgggatg
     8161 ttttccaaca gcaccatcag caactggcgt tcgatctcct gaatgacatc catcatccgc
     8221 ttaatgacct gcccggtgag atcctgaaaa tcctgcgcca tcatgatttc cagcagttgc
     8281 gcgttagtaa agctggtatg cgcgggtaca tctgccagaa atttccgcgt atctgttacc
     8341 agttcacggg cgtcggcaag gtcaatcgga tcggcaaacc agtcatccca acgttgggtt
     8401 aacgcttttg ctgatttctc catttgatcc tgatgcggtt gtgacgcctc aacactgttc
     8461 agcgcccgct ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg atcgcgcgca
     8521 tcggggatgg cttccgccgc ttcggcaatg gcctgatcca gtcccagttc ccgcaaactg
     8581 tcgcgcagca tacgcgtcag gctgccgatg cgcgcaatga tatcgccagc tgaatgctcg
     8641 tcagcaggtt tgattgatgg ttgcatcata gtcgcatcct cacatgccca gtttctcaaa
     8701 gattttgttg agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca catagccact
     8761 ggcccccgct tgcgccgcag caatgatgtt ctctttcttc gcttctgcag tcaccattaa
     8821 cactggcaat gccgacatcg cgccatccgc acgaatcgtt ttcagcaatt ccaggccatc
     8881 catgttgggc atgttccagt cggagataac aaatccataa ccgcctgcct gcaacttatt
     8941 gagagcgtcg acgccatctt ccgcttcctc aacattattg aatcccagct ctttcagcag
     9001 gttacgcact atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt taagttcttt
     9061 atccgccatt tcacactcct gatttaaata cgtatcgcct gtccggcact aatttttgcc
     9121 aacatttgct ggcttacctg gttaagatcg accacttcgc agacaccacc catattgatg
     9181 gcctcgcgcg gcatgccgaa caccacgcaa cttgcttcgt tttgcgcaag ggtccatgcc
     9241 ccagcctgac gcatcgccaa cattcccgcc gcgccgtcgt tgcccatacc ggtcaggatc
     9301 accccaaccg cattacgccc cgcctgtttg gcgacagaat ggaacaacac atctaccgaa
     9361 ggccgatgac ggttaaccgc cgggccatcg tgaattttga tttggtaatt tgcgccacta
     9421 cgcgccagct ccatatgccg atcgcccggc gcaatatagg catgccccgg caaaacacgt
     9481 tccccgtctt cggcttcttt aaccccgatc tggcaaagct tattaagtct gtcggcaaaa
     9541 gagcgggtga aaccgggcgg catatgctgg gtaattaaca gtgccgggct ggaaagcggc
     9601 aacggttgca gtacgtgacg aattgcctca gttccacccg ttgaagcacc aatcgcaatc
     9661 agtttttcag aactcaacaa cggccctgcc ttcagcgttg tcggtgccga caatggctta
     9721 tgtgctgcaa ggctcgcctt tgctgctgta cgcacctttt cagcaatcat ttcgctgtac
     9781 gccagcattc cttcgcgaat acccagttgc ggtttggtga caaaatctat tgcccccagc
     9841 tccagcgcgc gcagcgtgac ttctgatcct ttgccggtca gggaagaaac catcacaacg
     9901 ggcattggac gcaaacgcat taatttttcg aggaaatcca gtccgtccat ccgcggcatt
     9961 tcaacatcca gcgtcagcac atcgggattg aatttcttaa tcaagtcacg cgcgaccagc
    10021 ggatcaggcg cggtcgccac catttccatg tcgctatggc tgttgatgat ttctgtcatg
    10081 atctggcgca tcagtgccga atcatcgaca gataacaccc tgattttgct catcgttaat
    10141 ccttacttag cgcatacacc gtttgaccac gcagcgtgaa gcggcgctcg aggtggctaa
    10201 agttttcaga gtgacccgca aacagcaatc cgtcgggttt aaggagcgga acaaagcggc
    10261 gcaaaatctc ctgctgggta gtttgatcga agtagatcat gacgttacga cagaagatcg
    10321 catcaaacgg ccccggcacg gtgtactgtt tcgccagtag attcagcgag gcaaaatcaa
    10381 catagttcgc cagctcctga cgcacgcgta ccagcccttc atgcggtccc gtccctcgca
    10441 tgaaataccg ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg cgatagatac
    10501 cgcttctggc tttttccagc acttcggtgt cgatatcact ggcaaacact ttccagcgtc
    10561 cgggcgcggt gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg
    10621 tcgaagccgc cgcgctccat acgcgatact cgccagaacg gcgacgtgcg tgatccgcga
    10681 gcagagggaa atgatgtgcc tcacggaaaa atgccgtcag attcgtggtc agcgaattga
    10741 taaacgcctg ccactcaccg ctgtgctgat tagattccag caagttcaga taatgaccga
    10801 aatccgtcag tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt
    10861 tatggtcagc cagaacgatc ccggctcgtt gatagatcaa ttgacttatc cgccgaaaat
    10921 gcgcgtcgga aagcgccagg cgctcggtca tctgtaacaa taaagacgtt tgcccacagg
    10981 gcagagatga agtcatagcg ccttctcaat cacttcagga taccactggc gcaatttgta
    11041 actgcgccga ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc gccacacgcg
    11101 acgaaagatg gtcggcctga ttcgccagtt gttccgttgc caccgccgct tcttctacca
    11161 gcgaggtgtt ctgctgtgtc acctgatcca tctggctgac agcctgcgca acctgctcaa
    11221 tcccccgccg ttgttcttcc gaggccgaag caatttctcc cataatgtcg ttcacgcggg
    11281 tcaccgaact gacaatatcg gtcatggtcg cggcggcgtt attcaccagt ttcgaaccct
    11341 gctggacacg attgactgac tcttcgatca gccctttgat ctcttttgcc gcctgggcgc
    11401 ttcggctggc aagattgcgc acttctcctg ccactaccgc aaaaccacgc ccctgctctc
    11461 cggcgcgagc cgcttctact gccgcattca gggccagaat attggtctgg aaagcaattc
    11521 cgtcgataac gctgataatg tcgccaattt tttgcgagct ggtggcgatc tcctgcatgg
    11581 tgtgagtcat ggtactgacc tgaacaccgc ccgcctgcgc cgttgtcgcg gcattttttg
    11641 caagttccga cgcctgtcgt gcgttatcgg cgttttgccc taccgtggcg gttaattgct
    11701 ccatactggc ggccgtttgt gccagcgatg ccgcctgctg ttcggtacga cttgagagat
    11761 cgttattgcc tgcgacaatc tccgcgatac caatgtgcat ctcctggctt cccttacgca
    11821 catcacttac cgtcccacgc aaagcctgct gcatggtctt caggctggca aaaatggcgg
    11881 tgatctcatt acgaccatat accgcaatcg gacgcgccag attacccgca gcaatgctgt
    11941 caaaatggct accgataatg gccagtggtt gaacaatcat cttgcgcgtc caccacagcg
    12001 cactgctgat gtagatcgcc gcaacaataa tcatgctgat aaacaccagc gccgaaatct
    12061 gatagttacg ctggctttgc gcactggcgg cttccagcac atggatgatc tccagctgcc
    12121 aggcctcaaa gttaacgtca aacgcattct gtgatccctg caccggcgca gtgaggaaat
    12181 ccgaaagttg attactttca agccaggtcg cctgatgttc gagatcgttg tgccagcggg
    12241 caaaactttt ctccgtctct ttttgcaatg ccctgacgtg ctcgttgccc gcagtcatcg
    12301 ccataaaact tttaaacagc gtggtggatt gcgtcagact ggcacgcgcc gtcgtcatca
    12361 acgttttaat gtcatccgcc ggatagctaa gcgctgtcag agtgcctgct ttgttcagcg
    12421 tggtactggc ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt tgctgattgc
    12481 tctgctcgac ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa gacatgccgt
    12541 tactgccaat ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc
    12601 gaatacgatt aaacatcaac gctccccatc aggcggcaat gaccgcgtta gtaaatactc
    12661 gtcaaaatgt ttcccagttt ggatcttgtt cagcaattcg cagtcgtggc tgtgccggtg
    12721 gttgctcact ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg ctggctgcca
    12781 gacgaaacgc ggaaaccgct tgcgttaaac gactagcctg ttcttccagc gcagctgcag
    12841 cggcagctga ttcctgcacc agcgatgcgt tctgttgcgt gacgtgatcc atttccgaaa
    12901 ccgccaatgc gacttgatca atgccacggc tctgttcatc cgatgccgat gcaatctcgc
    12961 ccataatgtc agtcacgcga gtgacagcat cgacgatatt gttcattgtt tccccggcgc
    13021 tttcgaccag caccgaaccg gtatcaacgc gcgagacgga gtcttcaatg agagctttga
    13081 tctcttttgc cgcctgggcg ctacgactgg caagattacg cacttcgccc gccaccacgg
    13141 caaaaccacg gccctgttca cccgctcgcg cggcttcaac agcggcattc agcgcgagga
    13201 tattagtctg gaaggcaata ccgtcgatta cgctgataat gtcggcgatt ttcttcgaac
    13261 tatcggcgat ctcatgcatc gttttcacta cgccatccac cactttgccg ccgtgctggg
    13321 cggtgtcgga ggcactttgc gccagttgcg aggcctggcg ggcgttatcg gcgttttgct
    13381 tcactgtcgc ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg gatgcctgct
    13441 gttccgtacg ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta ccggcataga
    13501 tggcatctga accttcgcgg acatgagtga cggtgtcagt caaagagcgt tgcatatgtg
    13561 aaacgctctg cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg gtattcgcca
    13621 ggttaccacc ggcgatttcg cgaatgtgag caataatttt tgccagcggt gtaagcaaca
    13681 tacggcgaat gccataccac gccaccagca gaatcaatac caccaccagc gcgataaccg
    13741 ccagttgcca ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata tcgcgataca
    13801 gtttttcact gctgagggcg tactgagcaa acgcttcgcc cattgcattt tgcattccct
    13861 gggttggctg agcgaaataa gctccagtat tgccataatc aagataatca atcagttcag
    13921 ttaacgctgt gtaatagttt ttatattttt catcaatatt acgactggta gcgaccattt
    13981 caggtaacgg tgccatgctt ttgaattttt tataatgagt cgcggcctgg gccaatgttt
    14041 tcctggcgct atcgagcaat tcaactttgg cattactttg ttgattggag gaatccatca
    14101 tcattcgcac cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg
    14161 acgtcagctc gccctgctgt tctcgtaatt gattggaaac cacaaagctc ttctggctat
    14221 ggtgaaggga agaaaaaaac agactgccgg aaataagctg taacagtgcg aataccccca
    14281 gcaccattac caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa
    14341 acaagttgat ctcgttatcg gcaaggaagg gggaaacttt attgctgatg ccacccgccg
    14401 cgaaattgaa ataaaacccg atgcgcagat catcgggttc atttcaattg aggaaatcag
    14461 gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc gctgttcagc
    14521 agtttttcga tgttcaccag aatcaacata cggtcgccca gtgcgcccag tccagtgaga
    14581 tattctgttg aaagcgtcac ggcaaactct ggtgccggac gaatttgctc cgccgtcaat
    14641 gaaagcacgt ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc gagattcagg
    14701 acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt aattcgtaag
    14761 tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa cgctggcgtg
    14821 ttcgcaatcc gtgttacctg atcgtagcca cggatctcct gcactttcag gatatcaata
    14881 ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg ctcgctggcc
    14941 agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt caggcggcgg
    15001 tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca acaatcagtg
    15061 ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg cgatagttac
    15121 tttcaaggtt tttaactaca acctgatgtt gaccaattaa ttgatccacc agcaaggcgt
    15181 agcggcgacc gccactttgt aagatcacca caattccctg ggtagcttcg gttttcgcgc
    15241 ccgcgacgtt gaacactttc cacagttcga cgatgggcag atattcaccc cgcacttcca
    15301 gcacccgctc gccgccggcc agtggatgca gatcggcttc acggggttgc agtgattcca
    15361 taacagcatt cagcggcaga atgaatactt catccgcaac gcgtacggac atgccgtcga
    15421 ggatggccag cgtcagcggc agtaaaatgc ggatcgtagt gccagtaccc tgcttcgact
    15481 ggatttcaac atgaccgccc atctcctgga tattacgttt aacgacgtcc atgccgacgc
    15541 cgcgcccgga gacgtcggtg acctgctctg ccgtggagaa gcctggtgca aatatcagca
    15601 tcgcgacttc gtcgtcgctc atgttttcgc tgacagtcaa accttgcgag gccgcttttg
    15661 ccagaattcg ctcacggttt agccccgccc catcgtcggt cacttcaatg caaatgttgc
    15721 cgccctgatg ttcggcagac agaattaaat ttccgacgct gtttttacct gcggcgagcc
    15781 gtttttctgg cagttcaata ccgtgatcga ggctattgcg taccaggtgg gtcagcgggt
    15841 cgataatgcg ttctatcagg ctcttgtcga gctcggtgga actgcccacc agcgtcagtt
    15901 ctacctgctt gccgagtttt cccgccagat cacgcaccag ccggggatag cgactaaaga
    15961 catattccat cggcatcatg cgaatcgaca tcactgattc ctgcaaatca cgggcgttac
    16021 gttgtaactg ccccatgctg gtaatcaaat caccatgatt aaccgggtcc agttcgctgg
    16081 aacgctgggc aagcatggac tgggtgataa ccagctcgcc gacgaggtta attaattgat
    16141 caaccttttc taccgctaca cggatgctgg tggattcact gctacgcgtc gttttttccc
    16201 gctccacgcg accggttggc gcttgttcgg ctgccagttt aagcactggt ggggtggata
    16261 tttttggcga gacttctact gtttcaaagg taatctgatc ggcttcaatc acaaaacaga
    16321 gtaccgctgt gatgtcatct tcggcgatat cgcccggtaa tattgccgag agagaatccg
    16381 cccctttcac cacgtcagtt aacgttgtca gatgccccag ctcttcttcc agcaggtcga
    16441 cttcgctggc cttcaggcgc gaaaggataa ttcgtcgcgg caactgactg cgactctgct
    16501 catcttgcgg ttcacttttg gcaaccacac ttaatcgggt cactgcggat ggcgtttcgc
    16561 ctttcgcttc taatgccagt tgacgcaagg cctggcagat ataatcgaag ctggcggcat
    16621 ccggctcttg cgactgttta taagcgtcga gctgttcttg catgatgtcc ttcgtttcca
    16681 aaaacagatt gataatgtcg gtgttgagtt gcatctcacc tcgtctggct tcatcgagca
    16741 ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt cctgcccctc
    16801 ctttgatcga gtgggcagcc cgaaagatgg cattcaattg ttcggcatct ggcgcttccg
    16861 gctgcaaaac cagcagatgc tgctccatat cagccaacag ttcgtccgct tcatcaaaaa
    16921 atgtctgata aaaatcgctt atatccatgc tcacgctgtc acctcggttc ggctgatggc
    16981 attgtgggaa cactgacctg tggtgcaacc tcaggttttt ccagggcgct tactggctca
    17041 ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt cagtaccagc
    17101 aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg catcgttgcc
    17161 gccatgccga ccacacgtaa cactttgccg ctatccaacc ctccgaccat cagttcgcgg
    17221 cgggatgcat tggcccgatc ggcagaaagc tcccagttgc tgtaaccttt ctcaccactg
    17281 gcgtagggga aatcatcggt atgacccgaa aggctaatac ggttgggaat accattcaga
    17341 acgggcgcga tggcacgcag aatgtcgcgc atatagggtt cgacgtcggc actgccagtt
    17401 ctaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc ctggaccaga
    17461 tcgattttga gatgtggacg taacgcccgc agtttcggat cggactctat caattggtcg
    17521 agatcgccac gcaatttccg caatcgactt tgctccatgc gttttttcaa ctcttcgatg
    17581 ttcggctgct tattcacttc cccctggctt tgggtgtaat catcgccacc gccgggaatt
    17641 gggctttcac tattagaaat gcgatcgccg cccgtaaccg cagtcgccag tggagtccgg
    17701 aagtactccg caatctgaat caactctttt gggctggaga tggagatcag ccacatcacc
    17761 agaaaaaagg ccatcatcgc ggtcataaag tcggcataag caatcttcca cgatccatgt
    17821 gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg cgcttgattc
    17881 ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt caccgcacgc acatgctctt
    17941 ccagttcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca aactcaacgg
    18001 cgataggcgg tgcgtaaccg ttcagattag aaagcagtgt gactttgacg cactgcatca
    18061 ttttgctggt ttcggcgctt ttctgacgta aaacactcgc taatggggaa ataaatccgt
    18121 aagccaataa aatgccgagg aaagtcccca ccatcgcatg tgcgataagc gcccccagct
    18181 cggcggcagg acgatcggct gaacctaacg cgtgaacgac ccccattaca gccgcgacaa
    18241 taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg acttctgcct
    18301 cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc gatttcgaag gtgttcatgt
    18361 gaccgctgat aatcaggcgc agataatcga cgataaaatc aagcatgacg ctatccgcga
    18421 tgatgcgtgg gtagctggcg aagatctcgc tctcacgggg attttcaata tcacgttcca
    18481 gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga gccagcagat
    18541 ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc gctttcagcg
    18601 tgcctttaat cgcttttcca ttattgccga caataaacga cccaatccct gcaccggcaa
    18661 taatcaccag ttcagcgggt tgatagagtg ctccaaggct tccaccggtc atcaaataac
    18721 cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg acatcatcct
    18781 tccactgttg accatgacag gatgttcagt cgtcaggcgt taacgcgcga ttggggcaaa
    18841 aaaaagcagc ggtacgtcgt taccgctgct ggaatgttgc gccacaccgt atcagttaaa
    18901 cagcctgtac tctctgttca tccagcagtt gtgggataat atcggcagga ttctgggaaa
    18961 gtttacgtct ttttactgcc cgggatggcg gttgacataa gctgcaggca aagctgccaa
    19021 caggctggtg agcgtgggta ataaaattgc cgccgcagca gttgcagctg gaaagttgca
    19081 gtaatccact ttcaacaaac cgcaccaatg tccaggcacg ggttaatgcc agcagcggtc
    19141 cttcttctgc ttgcgggcac tgttcaaggt ataaacggta ggctttgatc accgcatcga
    19201 cgccattaca caaaccggtt ttcagtaaaa actgccatgc attacagaac atcgaagcat
    19261 gaacgttttg ttcccaagtc ataaaccagt cggttgagaa tggcagcatg cctttcggcg
    19321 gtgggcttcc gcgcagttct ttataaagtt ttatcaggcg tccgcgactt aactgtgttt
    19381 cgctttccag catctgcaaa cgagcgccca gggtgatcaa ttccattgcc agctgaatat
    19441 cccgcgcttc ctgaacaatg cttttttcac tcatgatcag gcccttttct tgcgcagcgc
    19501 ttcttcaggc tgattaacat cattcagcaa gcgtgttgag agcatgatgc cggtatgaat
    19561 ttgctggaga tcgtcaacgc gggaatcttg cgtcaacaga gtaatcgtct ggtggctgtc
    19621 aaaacggaag tgacaaacca gctgattggt ttctgccagc ttaaccattt gcggaagagt
    19681 cagtgccgct aatgttgtcg ccatttcttc atttatgccg agacgaaaca tagcggacgc
    19741 tttgtcctga acaatcagac gctgtgcaag tagtaaatat gacaagttga tgtcataaat
    19801 gtgtttcagc aactcggagg tatgcattat tcccacccag aataaccaac tttattttta
    19861 tgcggtttca ccgcaccccg tgatgtcgcc gggaagcccc ggtaaaaaat aattagcatt
    19921 agaatagttg cgataagctg caataagcag agccaccttt ttggtttaat aagtccttac
    19981 aaatagaagg gggtctttac acttatctaa gatttttcct aaatcgacgc aactgtactc
    20041 gtcactacac gcacatacaa cggagggggg ctgcgatttt caataatgtg tgatgcagat
    20101 cacacaaaac actcaattac ttaacataaa tgtataagtc atacttttgt tttgggtgta
    20161 tttcaatctg ttaaaaagtt tttcgctacg ctagcaagca aaaatgaaac aggaataatc
    20221 gaaatgggat gttgcgcaca gttaaaataa ctcaccgtaa ataatcatct gctataaata
    20281 atcactttca tgcaatacca gataagctat ttttaaacag acacttaccg cacaacaaac
    20341 taattaaata caaagaaagg caacaatcaa cttaatgtta ataaaatgtt ttgcatatat
    20401 taaccacttg tttaatgcgg ggaatcagta cattattttc ttataattac agttgaaatc
    20461 atatgatcac cgagagtgat tgattaattg tctttactaa taatcggact aatatttacc
    20521 tgtttgactg agttgggatt gcgtcgtttc tccattagga gtaaagcttt aatgtcacct
    20581 gaagttcaca gaataaggaa caggttatga gctatagcaa tattcttgtc gctgttgcgg
    20641 ttacaccgga aagtcagcaa ctgctggcaa aagcggtatc tatcgccagg ccagtaaagg
    20701 gacacatcag tttaattact ctcgcttccg acccggaaat gtacaatcaa ttagctgcgc
    20761 cgatgcttga agatttacgc agcgtgatgc aggaagaaac gcaaagcttt cttgataagt
    20821 taattcagga tgcggggtat cccgttgaca agacgtttat tgcctacggt gagttaagcg
    20881 aacatattct ggaagtttgt cgcaaatatc atttcgattt ggtgatttgc ggtaatcaca
    20941 atcacagttt cttttcgcga gcatcctgct cggcgaaaag agtgattgcc tcaagtgagg
    21001 tcgatgtgct gttagttcca cttacgggag attaaccgct cctacacaag cttaggaaag
    21061 gtagcaactt tatcgcgctg ctggctttcc gcgcttcgcg gaactatctg ctttaggtcg
    21121 ctaatgaagc actcctgcca gtggttaata tcgtttttca cgataacgtc cagcatttct
    21181 gcatgacggg aaatacgttc cgccagcgac atagtcaatg cacgatccag cgcaactgca
    21241 acttcgtcac gatcgtaggg attaacaatt aacgccgacg ttaactcgtt tgccgctccc
    21301 gcaaattgcg aaagaacaag aacgcccggg ttggctgggt cctgagcggc aacatactct
    21361 tttgctacca ggttcatccc gtcacgcagt ggcgtcacta agcccacgtc agagtagcgg
    21421 aatattttca tcagtaattt acggtcaaaa tgctgattca aataataaag cggcgtccag
    21481 cctaattgcc cgtatttacc attaattcgt ccggcttcat tttcgagctg atgacgaata
    21541 tcctgatagg cttgcacatc accacgcgac gttggtgcaa tctgggtata acgaatttta
    21601 ccatgatgct gcggatattt ttccagcaac gcttcatagg cgagaaaacg ctctggcaaa
    21661 cctttggaat aatccagccg ttcgacagaa aagatatttt gtacgttttt cagttccgct
    21721 ttaagttgcg ccagttttgg cggcagtggc ccggcagcct gtttggctat ttcttttggc
    21781 tcaatgccaa tcgggtaaac ttctgttcga aacgctttgc cccaggctgt atggcttttt
    21841 gcgctacgtg tcgtgacgcg ggtcaggtta gaaaggcaat ccaggaacgc cagacgatcg
    21901 ttttctgtct ggaaacccag caaatcataa tcacaaagct gttcaagcaa ggtgtcatat
    21961 gtcggcagcg cgttgaagat ttccggtgtc gggaaaggaa tatgcagaaa gaaaccaatg
    22021 cgattattca ctccccgttt gcgtaattca tgcgcaaatg gcaacaggtg ataatcgtgg
    22081 atccagataa tgtcatcgtc ttgcaacagc ggcagtaatt tatctgccag caacgcattt
    22141 acgcgtagat agccgtccca ggcaggacgc tgaaattgca ccagatcgag gcgataatga
    22201 aaagcaggcc agagaacggc attggagaat tggttgtagt attcgtcaag gtcctgttcg
    22261 ctgaggttaa aagaggccca cgtaatgtta ccctttttca ccttttttag cggctgatcc
    22321 tcattccctg tttcaccact caagccaaac cacagtccgc ctgcggcttt cagtgccccc
    22381 agtatgccaa cggcaaggcc accggcactg gcggcgtgct catctggtgg tgcaatccgg
    22441 ttagatacta cgactaaacg actcatagtc atcactcctg ttattttctc ttttttgttg
    22501 taatgcggtg gttatcattt caagccagct ccagacatcc ggtacgcccg ccagtcgcca
    22561 tgatgcctga gttgcgcctg tgccaatttt tactgacatt ccgcccagtc ggttaacgac
    22621 tgcgaagcca gattcatcgg ttaaatcatc gcccagaaat acgggcgttc gcccgataaa
    22681 gggagcttcc tgcataaagg cagcaatcgc ctcaccttta ctggtacctc tcggtttgat
    22741 ctcgacaaca cactttccct gctgtaacgc catttgtggc cagatctgag taatacgttg
    22801 cgctaatgtc attaatgcgt cttcatgctg cggagcctga cgataatgca gcgcaaaagc
    22861 catccctttc gtctccagct ccgcaccggg atactgggcg atgaccgtat gcagttgcac
    22921 gttaatatca cgtgcaatcg catccggcag atgaacgata tgcgttttac cattgatgtc
    22981 acggcgctcc gccccatgca cgcccgctaa cgggaagcga taaggttttg ccagtgcgtc
    23041 cagctccacc attgagcgcc ctgatatcaa tgccaatgca ccatcacttg cggttgccag
    23101 tagctgaagt ccttgcagaa tattgtcagg tacgacgacc tgatcgggat gcggtttgat
    23161 ttccgccagc gttccatcga gatcaaaaaa ccaggcatat ttcgcggata gttcaggggt
    23221 ttcggttaac ggttctgtca cccggttctc ctccttctct tttcttattg tgttgcgcag
    23281 aacgcgcttt acagacatgt aagtatagac agtgtgacgg gggtcgccat ttgcaacgaa
    23341 gaacagccaa ccgttgtggc ggctggctgt ggtgggaaaa aacgttaaat tgttgtggaa
    23401 aaaagcatca gacagtgcgt ttcgcttttt gcttgtaacg gtcgaagatc accgctgcca
    23461 gcaggattaa gccgcgaacc acgtactgcg cgaaaggaga aatattaagc aggttcatgg
    23521 cgttttccac ggtgcctaaa attaagatac ccgccaccac atatgagatt tttccgatgc
    23581 cacctttcag agaaacgcca cctaaaacgc aggcggagat aacaataagc tcataaccaa
    23641 tcgacgtcat tggctgccca ctggtcatac gtgaagccag aataattccg gctatcgcag
    23701 ataccaggcc agagagaaca aagataataa ttttggtgcg agcaaccggt acacccgcca
    23761 gacgcgcagc ctcttcgttc ccgccaatcg ccagggtatt acgaccaaag gtagttttat
    23821 tcagcagcaa accaaagata atcagacacg cgacggtgag ccagattggc gcaggcagac
    23881 cgaaccagtt ggcgtaacca agggcaaaga agctttcatc ttcgataccg accgctttac
    23941 cgtcggaaat gatgtacgcc agacctcgaa caatctgcat cgttgccagt gtcgtgatca
    24001 gagcatttat tttcagtttg gcgataacaa agccattgac caggccacag agaatgccaa
    24061 gcagcaaccc cgctgccacg ccaatccaca ggctttcagt cagattgata accaccgccg
    24121 tggtgacacc cgctcaggca attacggagg cgacagaaag gtcaaagtca ccggaagcaa
    24181 ggcagaacaa catgccacac gccaccatcc ccgacatgga aattgccagg cccaaccctt
    24241 tcatattaat aaaggtggca aagttcggga caaaaatggc acaggcgata aaaagcaccg
    24301 caaacaccac cagcatgccg tactgatccc agatacgccc gaagctgaat gacgacttag
    24361 gtgcgccaga acccgatgta gaaacagaag acatcatact ctccttactc aggcaactgc
    24421 ctggctgact ttaggcatcg caaggctcag tgcctgacgc tcatctgcct gctcgtgtaa
    24481 caattcaccg gcgatttcac cttcgcgcat caccacaatc cggtcggcaa cgccgaggac
    24541 ttcaggtaag tcgctggagg caaacagcac cgccacaccc tgcgccgcca gcgcataaat
    24601 cacgttatat atttcgtgct tagcaccaac atcaatgccg cgcgtaggtt catccagcaa
    24661 aatgaccttc atctcttccg ataaccagcg gcccagaatg gctttttgct gatttccacc
    24721 tgagagattc ataatcagtt gctcagcgcc cggcgttttg atgttgagcg aacgaatgtg
    24781 gtgatcggca ttgttttctt cccaaccgtt gttgattaca caaccgccga gcacatgttt
    24841 acgtctggca ctgatgttga tattgtcgcg aacggagtgc acaggaataa tgccttccgc
    24901 tttgcgatct tccgggcaga gcatcatgcc tgcggcaatg gcgtggctcg gtttacgaat
    24961 atcgatcggc tgttggtcga tataaacctg accggcggtg atttgcgtcc cgccaaacat
    25021 gcctttcatt aattcgctac gccccgcccc taccagacca aacagcccaa caatttcacc
    25081 actgcgaacc gccagactta ttggcgtacg cacgcctggt gctttcacag catcaagacg
    25141 caggcgctcc tcgccataac tacgcggttg ccagccgtag atatcgccaa tgtcgcgccc
    25201 gaccatcgcc tgcaccagcg cgtcgtggtc aacctgctgc atatcggtaa aggttttgac
    25261 ataacgtcca tctttaaaga cagtgatggc atcgctgagg gcaaatattt cttccatacg
    25321 gtgagaaacg tataagatca cccgcccctc ttttcgcagt tcacgaataa cgcggaaaag
    25381 attgtcgatt tcgcgggcag aaagggagct ggttggctca tcaaaggcga taattttagc
    25441 gttacgcgcc agcgccttgg cgatttcaac catctgccac tgaccaatgg agagatattt
    25501 cagcggcgtg tccgggtcaa tatccatacc aagatgttta agttgtaaac ccgcctcata
    25561 attcagcaat gagcgattca caatgccgcc tttatgcggc agctggccga gatagatgtt
    25621 ttccgcgacg gtcatttccg gcacgagatg cagttcctgg taaataatcg ccacacccgc
    25681 gttaagtgct gcggtcgtgt cggaaaagga catttcctgc ccattaatca ctacagaacc
    25741 cgtggttggc gcatagttgc cgctgaggat ttttaagaga gttgattttc ctgcgccatt
    25801 ttcacccatc aacgcatgaa cctgaccggc atagcagtca aaactaatat ccgtcagcgc
    25861 cttaacgccg ggaaacgttt taccgatgcc gcgaaatgag agatacgggg tagactgttg
    25921 cataacgtct ccgtgaatca ctggtcgttc acaccccctc tgcatcatgc aaaggggaat
    25981 ttttccggca aattacttac cgcctaaacc ttttttctcc agttcttctt taaagttgtc
    26041 acgcgtgatc agcaccacgt cggtaacttc ggtgaatttg gtcggttcaa caccttttgc
    26101 tacccagttg taaagcattt cgctggactt ataaccatgt acgtccgggc ttggcaacag
    26161 ggaaccgtag aagccggttg cctgtgcctt agacagttcg ctcaccgcat ccacaccgtt
    26221 aataccgata ccgatgatat cggccgcttt aaagccctga ccttccgtcg cgcgtacgcc
    26281 gcccagcacg gtgctgtcgt tcataccgac gatcagccaa tgtttaactt ccggatgttg
    26341 aaccagcatt gagttggcag cgtcaaatgc cccggggatg tcgttagatt tggtaggtac
    26401 ctgataaatt tgtttttccg ggaatcctgc agctttcagc gcatccatag aacccgtagt
    26461 acggcgacga gccgtatcca gttcgttgga ggtaatcgcc atcaccgcgc tttctttgac
    26521 atcccagcca cgtttctgca tctctttata cagttcctgg ccctgacgtt cgccaatttt
    26581 ggtcgccgcc atcatcacca gaggaacggt atccattggc ttacctttgg cgttaacaaa
    26641 ctggtcatcc acggcaatga ctttcatatc gtagccacgc gctttcgcca cgatggcaga
    26701 gccgagtttc gggtccggag tacaaataac gaaacctttt gcgccactgg cggccaggct
    26761 atcgatcgcg ttcaatgttt tttcgccatc cggcacggca atcttaataa cctcaaaccc
    26821 taaatccttc ccggctttat cggcaaactt ccattcggtc tggaaccacg gctcttccgg
    26881 ttgcttcacc aggaaaccga gcttcaggtt ctccgccata gcggattgtg acataacggc
    26941 tgccagacca atggctgcca gggctttagt aaatttgtgc atggttctct ccagctttag
    27001 tgtcgttttg tgcagggcaa aaacgaatga cattcgttaa attaatcgga aaacaaagca
    27061 ttacctttta actaaaagat aagtgactgt gttgacatag ttttagcgag aaattaattc
    27121 tccataggag agcaatatca catcgcagaa ttacagtgag aacgtgcata aatttagcgg
    27181 gaaaagacat aaggaaaagc caatttgtca gacaaattgt tgaatgcaca gcagattaat
    27241 ccataagatt agcctggaaa tccttgttgt ctttggtacc catgcgggat gtcttctttt
    27301 tcaccagtca ataggccgca ttacctggcg ttgagttttt gaaatggtgt aataaccgca
    27361 actcaaagat gtggaaaatg cacgtcattc atttcgtcat taattatcac tgtgctcatt
    27421 aattaacaga acacgtataa tgagagccat ctcgcaaaaa tgaaaaaacg ttttataaaa
    27481 taatcacttc atcatgaatt caaattcatt gattaatatc aacaagatac aaaaagcact
    27541 atcattaaaa ttcattgcag ttacattgat ttcatcaatg aaatgtaaaa atatataaac
    27601 ttgatgattt aagcattttc ttatacccgt tcagacgtta ttcttatttc agatcatcgt
    27661 cagaattgac tccacgatca catttcggac cggcagaaag gaattattct gcaaacagta
    27721 attatggtgt tttgatttat cttgcacctc tccacttctg gatataagga tattaggtat
    27781 ggcaaccgct ggaatgcttc tcaaactcaa ctctcaaatg aaccgcgagt tttacgcatc
    27841 caatctctac cttcacctga gtaactggtg ttctgaacag agtctgaacg gcaccgccac
    27901 tttccttcgc gcccaggcac agagtaatgt gacccaaatg atgcgcatgt ttaactttat
    27961 gaagagtgtc ggcgctaccc ccatcgttaa agccattgat gttcccggtg aaaaactgaa
    28021 ctctctggaa gaactgttcc aaaaaacgat ggaagaatac gagcaacgtt ctagtacgtt
    28081 ggcacagtta gccgatgaag cgaaagaact gaatgatgat tcaaccgtca atttcctgcg
    28141 cgatctggaa aaagaacagc agcatgatgg cctgttgctg caaaccattc ttgatgaagt
    28201 gcgcagtgcg aaacttgcgg gtatgtgccc agtgcagacc gaccaacatg ttctgaatgt
    28261 cgtgtcgcat cagctgcatt gatcatacat cgacgctaat gcattgcacc gatgaagttt
    28321 ttgagaaacc gctgcctcat cttttttgaa gcagcggttt tttaatggga ttcgccttgt
    28381 ggggtaaact tgagctcaat cagcgcgatg gctttttgga ttgcccgcat ggtgaccggg
    28441 tctgcggcag cgggatgatg agtaaagtcg atattcttca gctgactgga cattttttca
    28501 cgaacttcaa cgggcgcgat tacatcgaga acatccagaa tttgtttgat aaccaactgg
    28561 caagcaacca catcagaaac cagttcctga tcggcattca gcgtctggga catcgtaaac
    28621 tcctgatagc attttgaaag ccgttatagt agcggcttca catcttcaac gatagtcaca
    28681 tccaccctca tcaggacaca aaaaaacctg ccggagcagg ttttttgtta tcggaacata
    28741 ttgcctggcg gtacgtcttt gaacgtcttg caatagttat tgaacatact tttcaggatt
    28801 ttgcgcagtt tcatcgcggc actccgacca tttgttatac aggtgttatt gtctttgcgc
    28861 ttataatatg acaaccatca caaaaatcaa tctttatgtg atacaaatca cataaatacc
    28921 cctttaatgt tataaaaatg ataatcaaag aacagccccc tatttctgac acctacagat
    28981 ggcaagaaat agcgcctgcc aggcgtcttt tccggccatt gtcgcagcgc tgtaacgcgt
    29041 aaaatagtgc tttctcttac tcttctggct ggaccatgag acttctgatt ctgactcttt
    29101 cattaataac gctggcgggg tgtacggtta ctcgccaggc ccatgtgagc gaagttgacg
    29161 cggcaacagg tattgtacgg ttggtttatg accaggcttt tttgcagcat gcccataccg
    29221 atcgttatgt cagtcgcggg atctccgatc gcgcatgcca acaggaaggc tatacccacg
    29281 cgatcccctt tggtcagcca gtaggcaact gtagcctttt tgcgggttcc ttatgtttga
    29341 atacggaatt cactttgtca tatcagtgcc atcattccgc cttccctgtc tttctctaaa
    29401 tattttaatc agcgagggga tcttcgctga ttaaagaaat agcggtaatg cgttttattc
    29461 ccattcgtat ttttaataat tgaagtttat attttacctt ttgcaaataa taaaataaca
    29521 aattatagtg acgccacagc aacaaatata acctttgtgg agcactatca tgctgaaacc
    29581 agaaatgatt gaaaaactta atgagcagat gaacctggaa ctgtactctt cactgcttta
    29641 tcagcaaatg agcgcctggt gcagctatca taccttcgaa ggtgctgccg cgttcctgcg
    29701 ccgtcacgcc caggaagaga tgacgcatat gcagcgtctg tttgattacc tgactgatac
    29761 cggcaattta ccgcgtatta ataccgttga atctccgttt gctgaatatt cctcacttga
    29821 tgaattattc caggaaacct ataaacacga acaattaatc acccagaaaa ttaacgaact
    29881 ggctcatgct gcaatgacca atcaggacta cccaacattt aatttcctgc agtggtatgt
    29941 ttctgagcag catgaagaag agaaactgtt caaatcgatt attgataaat taagcctggc
    30001 aggcaaaagc ggcgaaggtc tgtattttat cgacaaagaa ctctctaccc tcgacacaca
    30061 aaactaatgc tctccggcgg caggtttctg ccgccatttc aaatcagtga cggcaaatct
    30121 tactctgatc ggtcgaaaaa ccttcttctg aaggtataaa tttgcctttt gctgccagaa
    30181 acgctaccag ctctcccgct gtcatccctt ctgccgagca agtgtgaaaa cgtgcctgtt
    30241 caccaaaacg cgctttaatc gcagcttcca gactggcatg cgtatattgc tcgcctgatt
    30301 caatcatcat atttaacact tcatgaccgt gaatagagtc catcgttcct cctcaaaaaa
    30361 aagcctagcg tagcgattgc cgcttatgaa gactttgcgc cagcgcagga ctgaatgctt
    30421 tttattgtac atttatgttt acaccatatg taacgtcggt ttgacgaagc agccgttatg
    30481 ccttaacctg cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa
    30541 aacagaaccc tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg
    30601 gcaatgccgc tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg
    30661 ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg
    30721 gcagataccg gtctgggcac gctggcaaaa cgctatctgg gacgctacgg tcaatggctg
    30781 acgggcttca gtatgatgtt cttaatgtat gctctgactg cggcatacat cagcggtgcc
    30841 ggtgaattgt tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct
    30901 ggcgtgttgt tgttcacttt tgttgccggt ggcgtggttt gtgtcgggac atcgctggtc
    30961 gatttattta accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta
    31021 ttgctgctac cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg
    31081 gcactgtctg caatcccggt gatttttacg tcgtttggtt ttcacggtag cgtgccgagt
    31141 attgtcagct atatggatgg caacattcgt aagctacgct gggtgtttat aaccggtagt
    31201 gcgatccccc tggtggcata tattttctgg caggtggcga cgcttggcag cattgattca
    31261 acaaccttta tgggattgct ggctaatcat gccggattaa acgggctgtt acaggcgtta
    31321 cgcgaaatgg tggcctctcc gcatgttgag ctggcagtgc atttatttgc tgatttagcc
    31381 cttgccacgt catttctcgg cgttgcgtta ggcttatttg attatctggc agatttgttt
    31441 cagcgttcaa ataccgttgg tggacgattg caaactggag caattacctt tctgccaccg
    31501 ttggcgtttg cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg
    31561 gcgctggcgg tactggcatt gattatccct tcgctgttga cctggcaaag cagaaagcac
    31621 aatcctcaag cgggttaccg ggtcaaaggt ggtcgtccgg cgctggtggt ggtgtttctc
    31681 tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg cagggttgtt accagaagtg
    31741 gggtgattag atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatt
    31801 tgacttctta atgcaggcag cactgcttaa attttttacc actaccgcac gggcaaggat
    31861 cgttacgccc cggtttctct tctgctttga tcggttgctg aacagctttt tcctgcggat
    31921 gcgccatcca gtacgcatgt agatcaagcg ccgccagtcg aatggcatct acgctctctt
    31981 caaacgcttc tggcgacatc ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg
    32041 ctatcgcctc cagcgctggt tttaacgaat cgggcaatgt tgaccagtca gaaagtgcca
    32101 cgccccgcat atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt
    32161 ctcgcaagcc gaataacggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat
    32221 cggccatatg ttgaaaagcc aggttcataa agcgcgtcat ctctttctct gacgcccagc
    32281 gcggcacata gtcagcccca ccccacacgg caaccagcca ctgtgccggt tcaatctctt
    32341 gcggagaact caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt
    32401 ggtcagtgtt gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg
    32461 gtcccgtttt catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc
    32521 cggtccgtat cagagatact ttttgagtgg ctttgccggt gattaaaaat caaggagggt
    32581 gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat
    32641 tgctttgatt ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag
    32701 tgcgaacgcc gagggatttt aaaaactatc aaccaccatt tataaatcat aaccttatga
    32761 ttttattaac tttgaaaatg gctctataca acgccatttg aatctattgt cactttttat
    32821 cgccactttt taccctctat tgtcaaaagg gttcaattca acagcagcct ctaaatgacc
    32881 tggagcaaaa tgcgcataac gcatagtcat ttttatatcg ctatgcccca gtattttttg
    32941 caacacaaga atatttccac cccgcatcat aaaatgactg gcaaacgtgt gacgtagcac
    33001 atgagttaat tgcccatcag gaagctcgat cttcgctctc ttaattgcag cgtcaaaagc
    33061 ctcataacat ggtgaaaata gcgctcctcg ttttttagga agcatagctt gcaattgagg
    33121 tgaaatcggt acagtgcggt tcttctttcc tttagtttta acaaatgtga ttcgaccggg
    33181 cagtacttga gattgcttta atccttctgc ttcactccac cgagcaccag tcgcaagccc
    33241 aatacggaca acaaccccca aatctttatt ccgtgactca tcacacgcaa tcagaaggcg
    33301 ttcaatctca tctacataca gaaacgccag ttccttttct tcctcacgaa acttgcgaat
    33361 accagtcagg gggttttcac cagaccactc cccaaggcgc ttcagttcgg caaaaacagc
    33421 atgtagatat gactgctcgc gattaacggt tgcttcacta agttttttct tccccttggg
    33481 attccattct cctgatagcc ttctttcccg ataagtagca aacatatttt tgtcaaactg
    33541 agaagcaaat ggatctccca gcctggaaca aatcgcctca agtttgactt tgcgctctgc
    33601 accagaggac aaggttttac cgtacatctc aaaccaacga gcaatcaact cagaaagacg
    33661 aggaccagaa ccatcttgaa actcgtctcc aactctacta ttcattaaac ggcgctcata
    33721 agagagcgcc tcactttttg tcgcaaactg tttacgaatg cgttttcccg atgccccgta
    33781 gggataacat tcacaaagcc atttacctga tggaatctta cgaaccgaca ttttagttac
    33841 ttatcacata aatcaaatgc agccttagtg acatccccca gactcttttt taaccctggg
    33901 gcggcatcat tatctagcca aaatggatta ttgttatcta acggtaacgc accaaatgtt
    33961 ttacctttta ttcgagccaa acctgtaagt gcatataact tattatcgtc aaaattcatc
    34021 acataaggat taccatcaag acactgtaat tgaacctcat cagtattaaa tggccatacc
    34081 ccattgaaac tctcacgttc aatagtttta aaaggcattg cgacggcgga aaaagaaaac
    34141 atagataaaa aagtaactaa tagttgaacc ttttttactt tcatatcatt acctcaattt
    34201 agctcaagta agattacaaa ttaaaaaacg ccctagaaat gacaccgcct accaaaaccc
    34261 ctacgcagat aaagaatatt atttcttttg gataaagtcg gattaattct gaagcacgaa
    34321 gtcggacttc tggtaaggtc gaactctcta tgtggcttga tgccgattgt tgctctaacc
    34381 acgacaatgc agactgtaac tgagaacgag taagatcgtt taaacgtcct gtaccgaaat
    34441 tgatatggca ataccgcaga agtttttgtc gaagtccaca gtcttcactg ttacgtagta
    34501 ataaacttac aagagcctta caggcatcat gatctttaca tcgctcaagc attgcatgca
    34561 gaaaactctc cgctgtttta tattgattta ctgtcatatc atcaatacca gctacaccaa
    34621 tctccgcatg tactttttgc caaataataa acgcttcagt attgctagct tctgcaatag
    34681 cagcaaccaa gctatttagc tccttacgct gagccttaag caaagggcga tcgtcatcat
    34741 cattattcga agggattgcg atattgacgg tatgagaacc atcatatcgc tctatctgaa
    34801 tattcttttc gtgaaaatca cgcccagcaa cgcgattgtt tgaaccgttt gagttgacgg
    34861 ccatgtcacc tccctactat cacctaccct tagtttcgtt atagtcacga ccagcgatac
    34921 ggttattacc accagaaata tttaactcac gtcctgatgg ctgagtttcc ttttcactga
    34981 tcgcaccttt taaagcccca atcaccgcgt ttttcacatc taacgaagct gctcgaaagc
    35041 gagtaatcaa ttcctgctca tcatcgttat aagtttcagg tgagtgaatt cccaacacaa
    35101 catactgaac atcaaggcca aaacgagaca gcgctgccaa atacgcagca tcaggaaagc
    35161 tatctccttt ctcatatcta agctgagtta gctttttgac tccaccaatg tcgctcatgg
    35221 caacttgact aagtcccaat ctttcccttt cctcacgcaa ccgccgacca atatcatttt
    35281 tcatacaaaa accttgacag gtatcttttt tgataccaaa atgatttcac gagctattag
    35341 atgatcacaa tataccacta tgaaacaagt tcttcacgat accagatcac gcattccgcg
    35401 taacaccgcc acaggtccaa gactggcact tcggctgtcc ctcgaggagc gagccgtcat
    35461 tgatgaaatg gcagctaaag aacaacgctc atcctctaac atggcgcgca tgatctccct
    35521 tcgcggccta gagctaaccc agaaagaaca aaacaaatct tcctgatcag gaggctagtg
    35581 ggatgtcagg tataaccatc aatatcaatg tgaatgcccc ctatgtatcc ctgcagaaat
    35641 atgcagagat aacaggtatc cctcttaata catgcaaaaa gatgttggct gacggtcgaa
    35701 ttattatccg ccccaaacgc gccaaaatgg aaaagcctga agtaaacctt gtggcgatgt
    35761 taaaagacgc tttggctaac agctaaaaca atgaacagag caccatcatg aaaaaaaacg
    35821 ctaataatcc atactccaaa tttcgtaatg gcgtagaacg ccatgtacac cacgtcgcta
    35881 ccagtgcatc acgtagtaac agtcgctata acctgaacga gacgcatgca acaccggatg
    35941 gccacgctgt aaaacaaatc ggcgaacatg cctggctgat tgagaaagct ggaatcgtgg
    36001 tccacaaatg cccacgcaat ccgtttaccg ggaaccgcat ttttgcattg aactgcggcg
    36061 acaatcactt cgggcaggat ttcacattat acgaagcact tcgcacggtt gatcgtctgc
    36121 ttcgcgggca aagttttatt aaacaggctg atttataaca ggtgctttat gaccaaagag
    36181 catgcacaag gtgtatttat ccgttttatt gattttcgcg gtgaactgtt attacgcgca
    36241 tcagccattg acggaattgt tccatccgaa aaaaacgcag ctacttacgt ttatctgaac
    36301 ggtacgcgcc tgactgtgga gcttccgtac cagaccgtac gagaaatcat tagcgaagct
    36361 gaaaaggctc gtcaggttaa tggcgatgaa ccctatatcg aaattatttg tatggattca
    36421 gaagctgaaa ttcagaaagc agattaaagg gcgttgcgat gggcaaagaa tataaaactc
    36481 tcattaacaa agcacctgaa cgattttatt ttcgcttaag tgcatcaggc gctcatgctg
    36541 aacgtgcagc ccgtgactca ttgaccaggg caatccggag tttgtatgac gttgcttttt
    36601 acgctgatga tctggatgca cttaacgaac tttccgagct gatctgtgcc gcagaatgcg
    36661 gggaacacat tgaaccatat aagctgggga atatcgcatg agtatattta tctcatggct
    36721 tgttctgatt atttcggtgg cctgcgccat tgggattatg cgaattattc attcagtaaa
    36781 aaagattgaa cgctttttca ctgacaaata acagcttaaa cagaacacca gattaaaccc
    36841 gaaaacctga aaactatccg cattcgcgga ggtattcgca cacgtaaata acggagatat
    36901 aaaatgaacg caaaagaaaa aaatattatc aatacattaa aaatagtgtc agcagagcaa
    36961 gacaagctgt ccagggcagc ccagaaagat aatcaacata tggcagcgct ttacgcactg
    37021 accatcgcaa tagccacccc tgaagcagcc aaagttattg aggaacagag caaagaaatc
    37081 gacactctta aaacacagtc aacagttgca gccatgaatc cgtccagcat tggacgctgc
    37141 atttatattc ttggttcagc aatgatgctg caatacacca ttattgccga actgcacggc
    37201 aaatacctga taacgcctta ccacacaaaa gagtcagagc ttctgacaaa tctccgcctg
    37261 atagaacgct ctcaagctgt attcattgat gacgcgcaac gtgccgtatt taacgcatag
    37321 ggttactgga caaagggggc gcaatggcaa ttaagcattt tcccgtcgtt cgctttacct
    37381 ccagagggcg cgaatacgag gtcaacgaac gcctgattac cactatcgac aaacatcgtt
    37441 cggaaaagga tgcacaccac atctacctca ctgacggcac ttacttctgc gccaccaacg
    37501 tggcgcgagt aaatcttatc cgacaggtac aggagccacg cagatgacca ttctggacta
    37561 catcgctact catccggggt gtagcggcgg agagatcgcc gcagcactga ataccccaac
    37621 cacagccatt aatgctgagt tacgccgact ttggcgcggc ggcttagtca tcagaacaaa
    37681 ccgcagcaca ggtggtcgcg ctcgcaaaac aggaggccag gcttcttacc acgtaaaccc
    37741 gatgccgttc gggtgtagca atccacttac tcacatgttt aaccagctac tgaaggaagc
    37801 cagagcatga gcaccatcaa ccaccagaag ctacgcgaac tggcatttgc cctgcaacga
    37861 atggcaacgc ctcaaaaatt actggcattt cgcgcaatgc tctcgccgtc tgctgtgctg
    37921 gcactgctgg atcagctgga gcacgccaga accacggctc ctgccattcg cctcacgctc
    37981 catcatgaaa tcgctgattt ctgcgcaacg ctgggggcac ctggcgaacc ggaaacgccg
    38041 gaagcaatgc agcaagagct gctgcaacgc atcgataacg tttttgattt ttttctgaac
    38101 cagtaagaaa ccagaacatg cacacacaaa aaaaccgctt gccatgccgc aatcggtcag
    38161 gttacatttc cgctgcacct cataaaacgg gtgccgggat tctcaacccg atacagagca
    38221 aagcgcataa ccgcgctagc ggtttttttg tgcgtactgt attgccacgt ctttttcgcg
    38281 tcagaattat ggcggggcgt acggggccga cttcggtcgg gccggattct ttgctctccg
    38341 gtgttgagaa ccctgtacgt ctcgccagcc cgagattctc aactctggat ggtgagttat
    38401 ttctatcacc gagcaaagag gccacaccat ggcagaccgc aaacagcacc gcgctatcgc
    38461 ggagcgtcgt cacatccaga ctgaaatcaa ccgcagactt tcccgcgcat cacgcgtcgc
    38521 gcaaatcatg cacatcaata tgctgcatga gcgcagccac gcactatcaa acatttattc
    38581 cgcctctgtt ttcagctatc tggcggatga tctgcacgag tttcaacagc tcatccagca
    38641 gcaaaacaaa ctccattaat tcctgttccg ggcctttcct gcaccttgcg gcgggaggcc
    38701 ttcgcacatc tgtaacaaga ggattgccgc aatgattctc gccaacgact ttcttgaata
    38761 cctgctcaac acagaacgtg atcttgccgc tcgcgtgcgt gatcgttatg acatgtacct
    38821 gaaatccctg cctgtaccgc agctcgctga cggaaagatt gttattgatg gtcgctacat
    38881 gattgacagc cacgagggaa attacaggct ttaccgcatt gaaggtggca ccccgtccgt
    38941 tattggcatt taccagcgcc catcctctgc aatcgtcgat gtgattgccg acagcatccg
    39001 catcacacat cgccatgccg acacagaaga caccgtgctg gaaattcagc ggctggctac
    39061 agcctgccgc gacaccctga atggcatgac gaagtaaatc actatgacgg cagagtacat
    39121 cagggactgg caacaaccgc gccacgcagt ggggcgtgaa ggaacgggga tccccgctcc
    39181 tgaatccgcg ctttcctcct ggctggatgc ctaccgggta gagaacgagc gccgccagga
    39241 aatggctgat gcggcgttct ccgcaacgcc gctgggcaac ctgattaata aaagcctgga
    39301 cgcacaggaa aaacaggaca aaaccatcac actggcagga gacgccagaa aacaggcacg
    39361 cggtgcggtg gatgaagcca tggcctcgct gcgcctgctg ccgtcctatc tgcgcgatcc
    39421 gcttattcgc cacctctcct tcctgcgcaa aaaacaggaa gccgatcgtc agaaaggcaa
    39481 aaagagctgg caggctgaac gctacgcgcg cggaaacctg cgcaaaatat tcgaacgtct
    39541 ggagcgcacc gatcaccgct ggctgacaca gggttatcgc tcccttgccg gacgcgaacg
    39601 cctggacgat ttgctttacc tgccgcagct caacaaacac cagatacaga cgctggccac
    39661 catgacggcg gcgatgttca gcagcacctt cgaaaaactc tgcgatggct ttggcgcgac
    39721 cgatggcgaa ctgaccatgg atgtaacgct gaaggcgtat cagatgctgg cccgcatggc
    39781 gttacacctg cacgccatgc ctccacatta tgacgcactg acaacagaca aagaccggag
    39841 gaacgaaccg gacacggagc tgctgccggg cgcaatcctt cgcctgacct gtgcggaatg
    39901 gtggaaacgc aaactgtggc tgttacgttg cgagtggaga gaagaacaac tccgcgccgc
    39961 ctgtctggtt tccagaaaaa catcgcccta tctgagccag gacgcgttaa gcgagtttcg
    40021 cgcacagcgc gagaaaacac gcgatttcct gaaaagtttc atgctggaaa acgaagacgg
    40081 gttcacgatt gatctcgaga cagtgtatta cgcgggagta agtaacccgg ttcaccgtaa
    40141 ggcagaaatg atggccacca tgaaggggct ggaacttctg gccgaagccc gtggcgacaa
    40201 agcggtgttt ctgactgtca cctgcccgtc aaaataccac gctacaacag agaacggtca
    40261 tccgaatccc aaatggaacg gggccaccat gcgcgactcc agcgattacc tggttaacac
    40321 gttttttgcg gcggtccgca agaaactgaa ccgcgacggc ctgcgctggt atggcatccg
    40381 cacggtggag cctcaccatg acggcaccgt gcactggcat atgatggtct ttgctcatcc
    40441 ggaagaaatc gacaccattg tgtcccacac ccgcgatatt gccattcagg aagatcgtca
    40501 cgagctgggc gatgatatta ctccgcgctt taaggcggag tatgtcgacg gctcaaaagg
    40561 cacgccaacc agctacatcg ccacctacat cggaaaaaac ctggacagcc gcgccgtgga
    40621 tggcatcgac ccgaaaacag gcaaaccacg cgttgaccac gaaaccggaa aatcaatgac
    40681 cgagagcgtg gaacgcgcca ttggctgggc gcgccttcac cgggtccgcc agttccagtt
    40741 ctttggcatc ccctcccgtc aggtgtggcg tgaactgcgt cgccttgcca gccagatggc
    40801 acgcaacccg gaaggcccgc aacggctgaa ggatgacgca atggatgcgg ttcttgctgc
    40861 cgctgatgcc ggatgtttta ccacctacat tgagaaacag ggaggcgtac ttgttccacg
    40921 caaggactac ctgattcgca ccgcctacga cctcgcagat gagctgaacg attacggcga
    40981 acagagcgta cagatttacg ggatctggtc accactcatc ggggaatcct cccgtgtgtg
    41041 cacgcacccg gataactgga agctggtaag acgtaaaccg ggagtagaag acagcgcccg
    41101 cgaaaatggt tttgaccttc agggcggccc tgccgcccct tggactcgtg gcaataactg
    41161 tccccgtgta caggaaacgg acaacaacgg gacagaacag ccggaagaac ggccagcacc
    41221 gtggccgcag cttcctgacg gcgttgacgt gaacgaatgg atgcgctcac tgaaacggca
    41281 cgaacgccgg gcgctgatgc gttcgcttcg tgacaaacag gcaaaaaaca gcagtgatga
    41341 aatgcagagc tggacacaga gccgcaaaca gcagcggcct ttgcctgata accacgaatt
    41401 actcgctaaa gaatggcggg agtctgctga atctctcggc ctgcatatcg gtgaacaaca
    41461 gatgcagcac ctgttacggg gcggcagtct gtacgttgac ggcagcatca ttgcaccgca
    41521 gggatttgaa attgtacgca aaccggatac ccgcccggac agccgaatca cgcagctctg
    41581 gcagcgcctg agccgtaatc atggcgtaag cagcacggag atccgccata acccggtcgc
    41641 cagctatctg gcacagctgg gggcatcaga ccctgaagcc gccgcacgcc tggcatccac
    41701 acttcagcag gaccagaaca ccatgaaaac acccgttacc gtgctttctg acatgctgcg
    41761 cgccatccgc gacgcagagc acgcacagag aatcagtgaa accactgaac gcgccagccg
    41821 caaagcagac ctgctgcggg gtggcctgac cagtggaaac aaaaaacaga cagaaacggg
    41881 actcacaaat cccgtaaatg agcaaaaaac gcgccgcgat atatgaagcg cgcacaaaac
    41941 aggcaaaagc gggatttaaa aatcctgtaa ccgattaatt aatcaacata aggaaaagcg
    42001 acatgaaaat ttgtatcgac gacggctcca ccaacatcaa gctggcatgg actgagaacg
    42061 gcgaacgccg caacgccatc agcccgaaca gcttcaagtc ggaatggtct gcgccattcg
    42121 gtggcacgca gcccgcgaac tacatgcttg atggcgtgcg ctatggtttt gatccggtca
    42181 gcgatcgctt tgtccagacg accgacacgc aataccagta cagcgatgtg aatgtaattg
    42241 ccattcatca cgcgctggtc aaatcaggca tcacaccaca ggaagtggat gtggttgtca
    42301 ccctgccact gagcgagtat ttcgatacaa acgcacagcc ggacatggcc aacatcaacc
    42361 gcaaaaaagc gaacgtcatg cgcccggtgg agtaccagaa cggcgaagca ttcactatcc
    42421 gtaacgtacg ggttatgcct gaatccattc ctgctggctt taaggcaccg gctgacatga
    42481 gtccgtttga atccctgctg attgtggatt tgggcggaac cacgctggat gtggcaaagg
    42541 ttcaggggca actggcaggt atcagccagg tgttttgcga tccacacgta ggcgtttctc
    42601 tgatggccga tgccgtactg tcggtgatgg ccactaacgg tatgcgcacc agtcaccaca
    42661 tcgccaatac cattatcgaa catcgccatg atgaagcctg gctgcgccag cacatccaca
    42721 atgacgcgca ttacgccagc ctgatggcgg ttattcgtga aaaggaagaa acactgaaac
    42781 aacgcgtgat ccgcgcgctg gcggtttttt cgggttacgg gcgggtgatg gttgtcggtg
    42841 gcggggcgga gattgtggca accgctatcc gcgaagcctg cggagttaat gcgactttca
    42901 tcgcggacgg ggtgccacag tttgctctgg ttaatgggtt gtacgcaatg aacaaggagt
    42961 aaaccaatga cgacaccaac cagacggata agtttctatc tgaagcccgc cgccgtcaag
    43021 agtgaacagg aggcgtgcaa ttacctcgat agcctgccag cctccgaacg cagccgcgcg
    43081 caacgcgcgg cctttctggc tgggctagca ctcataaagc gcaacccagc atttgcctac
    43141 tggatggccg aatggccaga ggacaacctc cccttcaata ccgttagcat caaggaaaca
    43201 taccaggcta atcagcctag cggagttgaa tgcaattttt ataaaataaa aaagaatatt
    43261 cagaccttat tcccagaata atctgataat tgtatgtgaa ctaataaaat ac
//
LOCUS       DAZKIF010000023        42376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZKIF010000023 DAZKIF010000000
VERSION     DAZKIF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.023"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            66..141
                     /locus_tag="ACHOTW_001319"
     tRNA            66..141
                     /locus_tag="ACHOTW_001319"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Lys,seq:ttt)
     gene            261..602
                     /locus_tag="ACHOTW_001320"
     CDS             261..602
                     /locus_tag="ACHOTW_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFP9843462.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(593..1519)
                     /locus_tag="ACHOTW_001321"
                     /pseudo
     CDS             complement(593..1519)
                     /locus_tag="ACHOTW_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1609..2607
                     /locus_tag="ACHOTW_001322"
     CDS             1609..2607
                     /locus_tag="ACHOTW_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP9843463.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2604..2822)
                     /locus_tag="ACHOTW_001323"
     CDS             complement(2604..2822)
                     /locus_tag="ACHOTW_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP9843464.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2824..4839)
                     /gene="ligA"
                     /locus_tag="ACHOTW_001324"
     CDS             complement(2824..4839)
                     /gene="ligA"
                     /locus_tag="ACHOTW_001324"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9843465.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4910..5908)
                     /gene="zipA"
                     /locus_tag="ACHOTW_001325"
     CDS             complement(4910..5908)
                     /gene="zipA"
                     /locus_tag="ACHOTW_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP9843466.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6138..6899
                     /gene="cysZ"
                     /locus_tag="ACHOTW_001326"
     CDS             6138..6899
                     /gene="cysZ"
                     /locus_tag="ACHOTW_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP9843467.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7084..8055
                     /gene="cysK"
                     /locus_tag="ACHOTW_001327"
     CDS             7084..8055
                     /gene="cysK"
                     /locus_tag="ACHOTW_001327"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9843468.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8439..8696
                     /gene="ptsH"
                     /locus_tag="ACHOTW_001328"
     CDS             8439..8696
                     /gene="ptsH"
                     /locus_tag="ACHOTW_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP9843469.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8741..10468
                     /gene="ptsI"
                     /locus_tag="ACHOTW_001329"
     CDS             8741..10468
                     /gene="ptsI"
                     /locus_tag="ACHOTW_001329"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP9843470.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10509..11018
                     /gene="crr"
                     /locus_tag="ACHOTW_001330"
     CDS             10509..11018
                     /gene="crr"
                     /locus_tag="ACHOTW_001330"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9843471.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPQLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11061..11912)
                     /gene="pdxK"
                     /locus_tag="ACHOTW_001331"
                     /gene_synonym="thiJ"
     CDS             complement(11061..11912)
                     /gene="pdxK"
                     /locus_tag="ACHOTW_001331"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP9843472.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12017..12384
                     /locus_tag="ACHOTW_001332"
                     /pseudo
     CDS             12017..12384
                     /locus_tag="ACHOTW_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            complement(12387..13298)
                     /gene="cysM"
                     /locus_tag="ACHOTW_001333"
     CDS             complement(12387..13298)
                     /gene="cysM"
                     /locus_tag="ACHOTW_001333"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP9843473.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13432..14529)
                     /gene="cysA"
                     /locus_tag="ACHOTW_001334"
     CDS             complement(13432..14529)
                     /gene="cysA"
                     /locus_tag="ACHOTW_001334"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP9843474.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            complement(14519..15394)
                     /gene="cysW"
                     /locus_tag="ACHOTW_001335"
     CDS             complement(14519..15394)
                     /gene="cysW"
                     /locus_tag="ACHOTW_001335"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP9843475.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(15394..16227)
                     /gene="cysT"
                     /locus_tag="ACHOTW_001336"
     CDS             complement(15394..16227)
                     /gene="cysT"
                     /locus_tag="ACHOTW_001336"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP9843476.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16227..17243)
                     /gene="cysP"
                     /locus_tag="ACHOTW_001337"
     CDS             complement(16227..17243)
                     /gene="cysP"
                     /locus_tag="ACHOTW_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP9843477.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(17401..18192)
                     /gene="ucpA"
                     /locus_tag="ACHOTW_001338"
     CDS             complement(17401..18192)
                     /gene="ucpA"
                     /locus_tag="ACHOTW_001338"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP9843478.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(18321..19178)
                     /gene="murR"
                     /locus_tag="ACHOTW_001339"
     CDS             complement(18321..19178)
                     /gene="murR"
                     /locus_tag="ACHOTW_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP9843479.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            19342..20238
                     /gene="murQ"
                     /locus_tag="ACHOTW_001340"
     CDS             19342..20238
                     /gene="murQ"
                     /locus_tag="ACHOTW_001340"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9843480.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            20242..21666
                     /gene="murP"
                     /locus_tag="ACHOTW_001341"
     CDS             20242..21666
                     /gene="murP"
                     /locus_tag="ACHOTW_001341"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP9843481.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            21671..22975
                     /gene="pbp4b"
                     /locus_tag="ACHOTW_001342"
     CDS             21671..22975
                     /gene="pbp4b"
                     /locus_tag="ACHOTW_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP9843482.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(23033..23932)
                     /gene="yfeX"
                     /locus_tag="ACHOTW_001343"
     CDS             complement(23033..23932)
                     /gene="yfeX"
                     /locus_tag="ACHOTW_001343"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP9843483.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(24028..24603)
                     /locus_tag="ACHOTW_001344"
     CDS             complement(24028..24603)
                     /locus_tag="ACHOTW_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP9843484.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24664..25113)
                     /locus_tag="ACHOTW_001345"
     CDS             complement(24664..25113)
                     /locus_tag="ACHOTW_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFP9843485.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(25100..25525)
                     /locus_tag="ACHOTW_001346"
     CDS             complement(25100..25525)
                     /locus_tag="ACHOTW_001346"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP9843486.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25739..26608
                     /gene="amiA"
                     /locus_tag="ACHOTW_001347"
     CDS             25739..26608
                     /gene="amiA"
                     /locus_tag="ACHOTW_001347"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP9843487.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26612..27511
                     /gene="hemF"
                     /locus_tag="ACHOTW_001348"
     CDS             26612..27511
                     /gene="hemF"
                     /locus_tag="ACHOTW_001348"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP9843488.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27517..28569)
                     /gene="eutR"
                     /locus_tag="ACHOTW_001349"
     CDS             complement(27517..28569)
                     /gene="eutR"
                     /locus_tag="ACHOTW_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP9843489.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(28615..29115)
                     /gene="eutK"
                     /locus_tag="ACHOTW_001350"
     CDS             complement(28615..29115)
                     /gene="eutK"
                     /locus_tag="ACHOTW_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP9843490.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29128..29787)
                     /gene="eutL"
                     /locus_tag="ACHOTW_001351"
     CDS             complement(29128..29787)
                     /gene="eutL"
                     /locus_tag="ACHOTW_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP9843491.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(29797..30684)
                     /gene="eutC"
                     /locus_tag="ACHOTW_001352"
     CDS             complement(29797..30684)
                     /gene="eutC"
                     /locus_tag="ACHOTW_001352"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP9843492.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(30705..32066)
                     /gene="eutB"
                     /locus_tag="ACHOTW_001353"
     CDS             complement(30705..32066)
                     /gene="eutB"
                     /locus_tag="ACHOTW_001353"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP9843493.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(32078..33481)
                     /gene="eutA"
                     /locus_tag="ACHOTW_001354"
     CDS             complement(32078..33481)
                     /gene="eutA"
                     /locus_tag="ACHOTW_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP9843494.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(33478..34704)
                     /gene="eutH"
                     /locus_tag="ACHOTW_001355"
     CDS             complement(33478..34704)
                     /gene="eutH"
                     /locus_tag="ACHOTW_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP9843495.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(34804..35991)
                     /gene="eutG"
                     /locus_tag="ACHOTW_001356"
     CDS             complement(34804..35991)
                     /gene="eutG"
                     /locus_tag="ACHOTW_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP9843496.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            complement(35981..36817)
                     /gene="eutJ"
                     /locus_tag="ACHOTW_001357"
     CDS             complement(35981..36817)
                     /gene="eutJ"
                     /locus_tag="ACHOTW_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP9843497.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(36828..38231)
                     /locus_tag="ACHOTW_001358"
     CDS             complement(36828..38231)
                     /locus_tag="ACHOTW_001358"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP9843498.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(38243..38530)
                     /gene="eutN"
                     /locus_tag="ACHOTW_001359"
     CDS             complement(38243..38530)
                     /gene="eutN"
                     /locus_tag="ACHOTW_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP9843499.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(38637..38930)
                     /gene="eutM"
                     /locus_tag="ACHOTW_001360"
     CDS             complement(38637..38930)
                     /gene="eutM"
                     /locus_tag="ACHOTW_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP9843500.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(38969..39985)
                     /gene="pta"
                     /locus_tag="ACHOTW_001361"
     CDS             complement(38969..39985)
                     /gene="pta"
                     /locus_tag="ACHOTW_001361"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9843501.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(39982..40713)
                     /gene="eutT"
                     /locus_tag="ACHOTW_001362"
     CDS             complement(39982..40713)
                     /gene="eutT"
                     /locus_tag="ACHOTW_001362"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP9843502.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(40710..41411)
                     /gene="eutQ"
                     /locus_tag="ACHOTW_001363"
     CDS             complement(40710..41411)
                     /gene="eutQ"
                     /locus_tag="ACHOTW_001363"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP9843503.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(41386..41865)
                     /gene="eutP"
                     /locus_tag="ACHOTW_001364"
     CDS             complement(41386..41865)
                     /gene="eutP"
                     /locus_tag="ACHOTW_001364"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP9843504.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(41878..42213)
                     /gene="eutS"
                     /locus_tag="ACHOTW_001365"
     CDS             complement(41878..42213)
                     /gene="eutS"
                     /locus_tag="ACHOTW_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP9843505.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT         9780 a        11889 c        10829 g         9878 t
ORIGIN      
        1 ctgggagagc acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc
       61 acttcgggtc gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt
      121 cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg
      181 cgatttgtaa gattgatttt tcttctcaag ttccttgttc ttctgccgat attacctcgt
      241 acagtgacga ggactgaaca atggccacta tctcttcaac ttcaattcct tccatacaaa
      301 cacagagtag taacagggca tcacaaggta gcgatgttgc ttcacaaatc gcgcggataa
      361 gtcagcaaat catcaaactc actcaacaca ttaaagagat tgtagacact agcggcagtg
      421 ctgaagacaa gcaaaaacag gccgaattga ttcaacagca aatcacccta ctggaaacac
      481 aactggcgca gttgcagaga cagcaggcag aaaaggcgca ggagaaagag cagcgtttgt
      541 ctctgaatgt atcccttcta aatcctgttg aaaatacgac tcatatcggt atctatatct
      601 gatacaacgg atccccttcc cgcccagcca ttagcgtatt gccctggtct cgtaccacgt
      661 cccataacgc ttcagcggcg gtggaaagtg aacggttttt cctacgcacc agcatcaact
      721 ggcgttccac caccggcgta atgcgcttta ccaccagcgg gctaccttca ggcaatggta
      781 gcgccagtgc gggaagaata ctgatgccga tgcctgcggc taccatcggg aacagcgtcg
      841 ccggatgtcc aatctcctgc acaatattcg cctgaatacc attgcgcgcc agcgctgcgt
      901 caatcagcgg tcggctgcct gacgcataat cctgcaacac caatttcgct ccctgtaatg
      961 cctgctaggg tacataatct tccgcggcca aagcgctatc ccgatggcaa agcagaaaga
     1021 aaggctcgga aagaatcact tcacattgca gatcgcccac tgggcctgga tcgatgacga
     1081 tgccaaagtc gacatctccc tggcgaatac tttccatcac ccactgctgc ggacgatcgt
     1141 gcaggacaaa ctgaatatct ggatagcggc gatggctttc agcaatgcat tgcggaataa
     1201 gatgcgcgga aatggtctgg ctggcagcga cccgaacttt tccgctcagt tgttgcccca
     1261 tacgcccggt atcgcgcaac gtgctgttaa gttcatccag cagtcgctca agacgcaaag
     1321 ccaactgctg ccctgcatct gtaagcacca cttcacgcgt ggttctgtcc agcaggcgaa
     1381 caccagtatg attttccagc tccttcacac tgtgacttac tgccgactgg ctcaggccga
     1441 tacgctctcc tgcacgacta aaacttttct cctgcgctac tgtgacgaaa acctttaatt
     1501 gttttaaaga ataattcatc tgttttcttc atgaatggat gcaataaatc aattttattt
     1561 ctcaaactgg aagaagcaca atagaaccat cgatcatctg gagtctttat gaaacttttt
     1621 cgtatcctcg atcctttcac cttaaccctg atcacggtgg tgttgctggc ctctttcttt
     1681 cctgccagag gcgatttcgt ccccttcttt gaaaatctga ccaccgcagc tattgccctg
     1741 ctgttcttta tgcacggcgc gaagttgtcg cgtgaggcga ttattgctgg cggtggtcac
     1801 tggcgactgc atttgtgggt aatgtgcagc accttcgtgc tgtttccgat tctgggtgta
     1861 ctgtttgcct ggtggaaacc ggtaaatgtc gacccgatgc tctactccgg ttttctctac
     1921 ttgtgcattc tcccggctac cgtgcagtct gcaatcgcct tcacgtcaat ggcgggcggt
     1981 aacgtcgcgg cggcggtttg ttctgcgtca gcatccagtc tgctggggat tttcctttca
     2041 ccattgctgg ttggtctggt gatgaatgtt cacggtgcag ggggcagcct tgagcaggtc
     2101 ggtaaaatta tgctgcaact gctgctgccg tttgtgttgg ggcatctttc ccggccgtgg
     2161 attggtgact gggtgtcgcg caataaaaaa tggattgcga aaactgacca gacgtccatt
     2221 ctgttggtgg tttatacagc gttcagcgaa gccgtcgtta atggtatctg gcataaagtt
     2281 ggctggggat cattgctgtt tatcgtggtg gtcagctgcg ttcttctggc tatcgtgatt
     2341 gtagttaacg tctttatggc acgccgactg ggcttcaata aggcagatga aattactatc
     2401 gtcttttgtg gttcgaaaaa gagtctggca aatggcatcc cgatggcaaa cattctgttc
     2461 cccacatcgg tgatcggtat gatggtgctg cccctgatga ttttccatca gatccaattg
     2521 atggtctgtg cggtgctggc gcgtcgatac aaacgccaga ccgaacagtt acaggcgcag
     2581 caggaaagca gcgccgataa agcttaaagc ggacgcttca ggggctggac cagttgcgtc
     2641 agcccctcgg ttttaatcag cagcgtgatt tgcattagct caccgagctt ccctgccggg
     2701 aactcatctt tgcgggcaaa ccacagcaga tactcctccg gcaggtcgat taagcgacgc
     2761 cctttgtatt tgccaaacgg cattatcgta ttggctattt caatcagctg ctctttttcc
     2821 atctcagcta cccagcaaac gcagcatttc cgcttcgtcg atgacttcaa tgcccagttc
     2881 ctgcgccttc gccagtttag atcctgcagc ttcacccgct atcaccagat cggttttctt
     2941 cgacacgctg cccgcgactt tcgcgcccag ttcgaccagt cgagctttag cgtcatcacg
     3001 cgacatctgg cttaagctgc ccgtaagcac cacggtttta ccagcaaacg ggctgtcaat
     3061 ctcttccgcg ttgataacga tcggcgcagg ccagtgaaca ccttccgcca acagctcgct
     3121 gatgacattg cggttgcttt cttcggcaaa gaagttgtga acgtgggatg caacgacaat
     3181 gccaacatca ggcacctttt gcagctcttc aatcgaagcg gcttccagcg cttccagcgt
     3241 tccgaaatat gccgccagac ctgctgcggt ggcctcgccg acttcacgga tgccaagtgc
     3301 atagaggaag cgagcaaagg tggtttcttt cgctttttcc agcgcgttaa ccacgttttg
     3361 tgccgatttt ggccccatac gctccagtcc ggtcagtttg cctgcggtga gtttgaacag
     3421 atccgccgga gtgtggacat attctttttc aaccagctga tcgatgattt tgtcgcccat
     3481 tccgtcaaca tccatcgcac ggcgggaaac aaagtgtttc agcgactctt tacgctgcgc
     3541 accgcaaatc agaccaccag tacagcgggc aaccgcttca ccttccacac gctcaacgtc
     3601 agaaccacat accggacaat acgtcgggaa tacaacctca cgggtatctt ccgggcgttc
     3661 agaaagcacg acgttaacca cctgcgggat cacgtcgcca gcgcggcgaa tcaccacttt
     3721 atcgccaatg cgtaaaccaa gacgttcgat ttcatccgca ttgtgtaagg ttgcgttact
     3781 caccagcacg cctgcaacat ggacaggttc cagacgcgca acaggcgtaa tcgcgccagt
     3841 acgcccaacc tgaaactcga cgtcacgcac aaaggtcatc tgttcctgcg ccgggaattt
     3901 aaacgctacc gcccagcgcg gggctcgcgc gacaaagcca agctgctcct gctgttccag
     3961 tgagttgacc ttaatcacca cgccgtcgat atcaaagccc agcgtcgggc ggtcttcttc
     4021 cactttgtga tagaacgcca gcacttcttc cgccgattca caaagcgtta cccgatcgct
     4081 gaccggcaac ccccactttt taaattgcag taaacggcca agatgagtat ccggcagctc
     4141 gccaccttcc agaacaccaa cgccatagca gaaaaaagtg agcggtcgct tcgctgtaat
     4201 acgcggatca agctgacgca gtgaaccagc tgccgcatta cgtgggttag caaacacttt
     4261 cccgcccgtg cgtcgcgcat cttcgttaat cttttcgaac cccgcctgcg gcaggaacac
     4321 ttcaccacgc acttccagac gcgccgggat attctctccg tgcaacttca gtggaatggc
     4381 gcgaatagta cgcacattag acgtgatatc ttccccggtg gtgccatcgc cacgagtcgc
     4441 ggcactgact aaaacgccat tttcatacag aatactgacg gcaaggccat ccagcttcag
     4501 ctcacagcac caggtgactt tctcgttgct tttcagacgg tcctgcacac gtttgttgaa
     4561 ggcaagaaag ctttcttcat caaaaacgtt atccagcgac agcattggca cttcatggcg
     4621 aatctggctg aaagccgcca gcggcgcagc acctacacgt tgggtaggcg aatcaggcgt
     4681 aatcagttct ggatgtttgg tttccagctc gcgcagttcg cgcatcagcc tgtcgtattc
     4741 agcgtcggga atttccggcg catccatcac atgataaaga tattcatgat ggcgaagcgt
     4801 cgttcgcagt tctgtcagtt gttgttcgat tgattccata tcgcaccatc aatgctaaaa
     4861 acccccgaca agcgggggtt cgaagaggag ttaatttgcc ttaagtgtat caggcgttgg
     4921 cgtctttaac ttcgcggatg atgtcctggt actcgcgcaa tttctgcgga gtcatcatac
     4981 ggcgctgatc gtcaagcacg acaccgccca cttcatcggc aatatgctgc gcagattgca
     5041 gcatcagctt gaagttctgc agctcgtcac cgtaagacgg tacctgcata aagatagtga
     5101 cacccggagt agtgaaatcc ttcatttcag gatcaaaggt tcccggtttc accatattcg
     5161 ccaggctaaa taacgccggg ccgctgccat ccgggctaag atgacgatgg taaatattca
     5221 tatcgccaaa aatgaagccc gcttgttgaa tgctgttaag aagcagttca ccgtttagct
     5281 cgctaccgtg atgcgccgcg acgttcatga taatcaccgc ttctttgcgc ttcggtttat
     5341 ccataactgg agccggttcc gctacaggct caggctgtgg tgccgctacg ggttctgcag
     5401 gctggaaagc ctgttgtgcc ggttgcggtg ctgaatgcac tggctgcggc gctggcgcga
     5461 cctgtggcga aactggctgc tgcaacggct gttcaggctg cggctgatag gcaggctgct
     5521 gcacgggttg ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt
     5581 caggcggctg ctggaccggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt
     5641 gttgcggcga cggacgagca gcctcatgct cctgggcgtt agccggggca tgattcacgc
     5701 ggtgaacacg aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt
     5761 cgtcacgttt tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt
     5821 tacggctggt ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga
     5881 ttaatatcag acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc
     5941 acggcaaaca tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt
     6001 aactttcaca gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc
     6061 taactgtcca ttgcgcaatt acccggtaaa atacgcacaa ttttcctggg attggtcaaa
     6121 aggagctcat cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact
     6181 attttgcgca aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac
     6241 cgctgctggt caatattttg ttgatggggg gcgcattctg gtggctcttt acgcaactcg
     6301 atgtctggat cccgactctc atgagttatg ttccggactg gttacaatgg ctgagctatc
     6361 tgttgtggcc tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga
     6421 ttgctaactg gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat
     6481 tgactggcgc tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa
     6541 tcatgaaacg cgaatggcaa aaatttgcct ggtatctgcc acgcgcaatt gtattgctaa
     6601 ttctttactt catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta
     6661 gcgcctggat gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt
     6721 ttaaagagat gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt
     6781 taaccagtct gtttacgatg atcccgctgc ttaatctgtt cattatgccc gttgccgttt
     6841 gtggcgcgac ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac
     6901 aatctaccgg ttattttgta aaccgtttgt gtgaaacagg gggggcttat gccgcccctt
     6961 attccatctt gcatgtcatt atttcccttc tgcatataga tatgctaaat ccttacttcc
     7021 gcatattctc tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag
     7081 gccatgagta agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc
     7141 ctgaatcgca tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc
     7201 agcgttaagt gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg
     7261 aaaccaggcg ttgaactggt tgaaccaacc agcggtaata ccgggattgc actggcctat
     7321 gtagctgccg ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa
     7381 cgccgcaagc tgctgaaagc gttaggtgca aacctggtgc tgaccgaagg tgctaaaggc
     7441 atgaaaggcg caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg
     7501 ctgctgcaac aattcagcaa tccggcaaac cctgaaattc acgaaaagac caccggtccg
     7561 gagatctggg aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc
     7621 ggtacgctga ctggcgtcag ccgctacatt aaaggcacca aaggtaagac cgatcttatc
     7681 tctgtcgccg ttgagccaac cgattctcca gttatcgccc aggcgctggc aggtgaagag
     7741 attaaacctg gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc
     7801 gatctcaagc tggtcgataa agtcattggc atcaccaatg aagaagcgat ttctaccgcg
     7861 cgtcgtctga tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgct
     7921 gcggcgttga aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta
     7981 ccgtcatcgg gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa
     8041 gaattgcaac agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt ttcaggtgct
     8101 tttttgtggc ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg
     8161 gtatttaacc agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag
     8221 ctgaatcgat tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt
     8281 acgaaacgtc agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg
     8341 cgtctgttaa aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt
     8401 agttccacaa cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca
     8461 ttaccgctcc gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg
     8521 gcttcacttc tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt
     8581 ttaaactgca gactctgggc ctgactcaag gtacagttgt gactatctcc gcagaaggcg
     8641 aagacgagca gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc
     8701 ccgggttctt ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc
     8761 atccccgggt atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga
     8821 ccggaaaaaa atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg
     8881 tgccaaggca tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga
     8941 agaaaaagaa gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca
     9001 ggaaatcata gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt
     9061 tatcgaaggt caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc
     9121 ggctgacgta cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat
     9181 cgacctgagc gccattcagg atgaagtcat tctggttgcc gctgacctga caccgtccga
     9241 aaccgcacag ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac
     9301 ttcccacacc tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag
     9361 cgtcacctct caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt
     9421 ttacgtcaat ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc
     9481 ttctgaaaaa gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca
     9541 ccaagtagaa gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa
     9601 cggcgctgaa ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct
     9661 gcccactgaa gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca
     9721 ggcggttatc gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt
     9781 cccgaaagaa gagaacccgt tcctcggctg gcgcgctatc cgtatcgcaa tggatcgtaa
     9841 agagatcctg cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg
     9901 cattatgttc ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga
     9961 aatctacaaa caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg
    10021 cgtaatggtg gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga
    10081 tttctttagt atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa
    10141 tgatatgatt tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca
    10201 agttattgat gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg
    10261 cgatgaacgt gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc
    10321 catttctatc ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt
    10381 gttagcagag caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa
    10441 gttcattgaa gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg
    10501 agaagatcat gggtttgttc gataaactga aatctctggt ttccgacgac aagaaggata
    10561 ccggaactat tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc
    10621 cggatgtcgt ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta
    10681 acaaaatggt cgcgcctgta gacggcacca ttggtaaaat ctttgaaacc aaccacgcat
    10741 tctctatcga atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg
    10801 aactgaaagg cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg
    10861 atactgtcat tgaatttgat ctgccgcagc tggaagagaa agccaagtct accctgactc
    10921 cggttgttat ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtga
    10981 ctgtgggtga aaccccggtt atccgcatca agaagtaatt cttgctgcag tgaaaaatgg
    11041 cgcccatcgg cgccattttt ttatgcttcc gccagcggcg gcaaaatcaa ttcatcgctc
    11101 tcatgctgct gggtgtagcg catcacttcc agtacgcgca accccgcgcg gtgcaccgca
    11161 tcgtttaatg ccttcccttt cagcaaacca ctgataagct gagcacaaaa caggtcgcca
    11221 gtccctttca ggtcggtttt tacccttgaa tgggaaatga cattcacgct gtcggcagtg
    11281 accaccacaa ccagcatctc ctgattttct tcattaccgg aggcgctggt aatcaccacc
    11341 cattttagtg tgtctgaaag cagacttttt gcggcagcaa tggcactgtc gagatcgcgg
    11401 caatttttac cggtcaggat ttccaactca aagatattgg gggtaattcc ctgcgccagc
    11461 ggcagtaaat attgtcgata cgcttcggga aggtcaggtt tgacataaat tccgctgtca
    11521 atatcgccaa tcaccggatc gaccatgatc aataggtcag gatggtcttt gcgtagcgca
    11581 gtcagccact cggcaaggat tttgatttgc gatgccgttc ccatatagcc cgtggttaca
    11641 gcacgaagtt ggcgcagcgc atcacgctcc tgaagtgcac gcaaatagcc gctaaaccat
    11701 tcatccggaa tcgcaccacc gtagaaagtg tcataatgcg gcgtattgct cagcaatacc
    11761 gtcggcacgg caaagacatt caggccgttc tgtttgatag caggcacggc aatgctgttg
    11821 cccacgctgc cgtaaaccac ctgcgactgc acggcgacga tatccgcctg cagcgccctg
    11881 ctcttatcgt taaacaacaa caaactactc atttaatttt ttctccttgc cgatgatcct
    11941 catcgtattc caaccgaaac tttacctgat tctggcagtc aaatcggcta ttacaaaaca
    12001 aggataaggt aattcagtga agaaaatcat ttgtctggtc attacactac taatgacact
    12061 ccccgcttac gcgaagttaa ctgcccatga agaagcccgc atcaacgcca tgctggaggg
    12121 attagcacag aaaaaggatt tgatatttgt gcgcaacggt gatgaacata cctgcgatga
    12181 agcggtttct catctgcgtc tgaagctggg caatacccgt aaccgcattg acactgccga
    12241 gcagtttatt gataaggttg cttcgtcgtc atcgattacc gggaagccgt atattgtgaa
    12301 gatccccggt aagagcgatg agaatgcaca gcctttttac atgcgttaat tgcgcagacg
    12361 gataaaacgg tgcctgcgca ataatcttaa atccccgccc cctgactaaa atgctcttcc
    12421 ccaaacaccc cggtggaaag gtagcgatcg ccacgatcgc agatgatcgc taccaccacc
    12481 gcaccagggt tagcttttgc cacccgcagt gctccggcaa ccgcgccgcc ggagctgacg
    12541 ccacagaata ttccttcccg caccgccagt tcgcgcatgg tgttttccgc atcgcgctga
    12601 tgaatatcca gcacctcatc taccagagaa gcgttgaaaa tccccggcag atattccgca
    12661 ggccagcggc gaatgccggg aatgctactg ccctcttccg gctgcaggcc aacaatggtc
    12721 accggtttgg attgttcgcg cataaagcgt gaaacgccgg tgatagtgcc ggttgttccc
    12781 atgctggaga caaaataggt aatgcgcccg ccagtctgct gccagatttc cggcccggta
    12841 gtggtgtaat gggcgtaagg gttatcggga ttattgaact gatcgagcag ctttccttca
    12901 ccacgatccg ccatctccag cgccagatcg cgcgcacctt ccatgccctg ctctttggtg
    12961 accagaatca gttccgcacc ataagcacgc atcgccgcac ggcgttcctg gctcatgttg
    13021 tcgggcatca gcaatttcat gcgatagcct ttaagcgcgg caatcattgc cagcgcaatg
    13081 ccggtgttac cactggtggc ttcgattaac acatcacccg gtttaatttc cccgcgcttt
    13141 tccgcctcga cgatcatcga aagcgccgca cgatctttta ccgaacctgc cgggttattg
    13201 ccttccagtt ttaaccacac ttcactgccg ttattcggcc ccattcgctg caacttcacc
    13261 agaggcgtat tgcctattgt ttgttctaat gtactcacga tctctatcca tacgtggtgt
    13321 tgcctgatgc gacgcttgcg cgtcttatca ggcctacagg ttacaaacct tgccattaaa
    13381 aagcccggat cgcgggaagc gcctccgggc gtttaacatt cactcaacct atcaggcgct
    13441 ttgtgcgaga gcaagttcct catctcgggg ttcgatacgc tcgtcgccgt tatacagccg
    13501 cgcatgttgc agaccaacga ataaacgctc gccacgctgc ggggcatcgt cgccatgcat
    13561 cacgaccgtc agcggttcgt tgtaccaccc cagcggctgc accactaatt gggtgtagtg
    13621 acctttcggg ctggcttcca gtacctgtac cggcagcggc gaatcgaggc tggtacggcg
    13681 gctgatatcc acttcccacg ggcgcaggaa gagatccacc ggcccctgat acgcaggtgt
    13741 gtagcccagc ggccagcgat gtgcgccaac atggaactgc ccgccgcgaa tggttccctg
    13801 caggcggttc acttcgccca taaattcgag cacaaaacgg gtcgccggtt cgcgccatac
    13861 ctgatccggc gcgtcagcct gttcaatatt gccctggctc atcacaacta cacgatcagc
    13921 tacttcggtc gcttcttcct gatcgtgggt cacaaaaacg ctggtgaatt ttagttcttc
    13981 atggagttga cgcagccatc gacgcagctc tttacgcacc tgcgcatcca gcgcgccaaa
    14041 cggttcatca agcagcagaa tttgcggttc tacagcaagc gcacgcgcca gcgctacacg
    14101 ctgtttctgg ccgccggaaa gctgcgccgg ataacgatcc gccagatggg caagctggac
    14161 catttccagc aattttgtca ctttcgcttt gatggctgcg gcattcgggc gctcgcgacg
    14221 cggcagcacc gtcaggccaa aagcgatatt gtcgaacacc gtcatatggc ggaacagcgc
    14281 gtaatgctgg aacacgaaac cgactttacg atcacgtgcg tgcaggcggc tcacgtcggt
    14341 gccgtggaag cgaatatgcc cgctggtttg atgctccagc ccagcgataa tgcgcagcag
    14401 cgtggttttc ccggaaccgg acggccccag caacgcgacc atctgacctg aaggaatatc
    14461 cagtgagata tcgttcagca cctgggtgcg accaaacgac ttcttaatat tggcaatctc
    14521 aatgctcatg attttcctcc tgctgcgcgc gtttttcctg attctccagg cgccactgca
    14581 acatactctt taaaaacagg gtgataatcg ccatcagcgt caacagcgcg gcggcggtaa
    14641 aggagccgac tgtgttgtag tcctgctcca gcaattcaat ctgtaacggc agcgaaagtg
    14701 tttcgccacg aatcgagccg gaaaccaccg acaccgcgcc aaactcgcca atcgcgcggg
    14761 cgttggtcag cactacgcca taaagcagcg cccagcggat gttcggcaat gtgacacggc
    14821 ggaacatctg ccagccggat gcgccaagca aaatcgccgc ttcgtcttcc tgactgccct
    14881 ggcttaacat caccggcacc agttcgcgca ccacaaacgg acacgtcacg aagatcgtga
    14941 ccagcaccat tccgggccag gagaacataa tttgcagatt atgttcatcc agccagccgc
    15001 cgagcgggcc gttggagccg tagaacagca gatacaccag accggcaacc accggtgaca
    15061 cggcaaaagg aatgtccagt agcgtcagca gtaactggcg tccggggaag ttaaagcgcg
    15121 tcaccagcca ggccagcaga ataccgaaca ccaggtttac cggtacggca atcagcgcga
    15181 tcatcaccgt cagccagatg gcgtgcagca tatccggatc ggccagattc tgtaaaaccg
    15241 gcatcagccc tttgctgaac gcctgtacga agatataaat catcggcacc agcaggatga
    15301 acgccgatac cagcatcccg atgccaatca gaaaccattt gccccagtta atcgggcgcg
    15361 cgtcataacg cttcaattgt gtcacttccg ccattaatga cctaccacac gccgaccaaa
    15421 gcgactttgc agagtgttaa ttgagaacag cagcagcaga gatgccgcga ggatcaccga
    15481 agcaatcgcg ctcgccgccg ggtaatcaaa ctcctgtaag cgaacgaaaa tcatcagcga
    15541 cgtcacttcc gtcttccaag cgatgttccc ggcgataaat atcaccgcgc caaactcacc
    15601 aagactacgg gtaaacgaca acgccacgcc cgccaccagc gccggagaaa gttccggcag
    15661 caccactttg cagaaactct gccagcgcgt tgcgccaagc gtttctgccg cttcttcata
    15721 ttccgggcct aactcttcca gcaccggctg cacggtacgt accacaaacg gaatgctggt
    15781 aaaggccatt gccaccgcaa tccccagcca ggtataggtg actttgatat caaacttcgc
    15841 cagccattca ccgtaaaaac cgtttacaga aaagagcgag gccagcgtta aaccggcgac
    15901 tgccgttggc agcgcaaagg gtaaatccat cagcgcatca agcagcgtgc ggcccggaaa
    15961 gcgataacgg gttaaaatcc acgccatcag caggccgaaa acgccgttaa aaattgatgc
    16021 cacaaacgcc gacagcagcg tcactttgta ggccgcgacc acctgcgggt tggtgatcac
    16081 ttcccagtac tgcgcccagc tcatctcaga aagctgcatc accagcgcgg agagtggcag
    16141 cagcaatatc aggcacacaa acagcagact ggtgccgagg cttaaggtaa agcccggcag
    16201 gacgcgtctt gatgaaactg caaacattaa ttacgccccg ccgctaacag cttgtctaac
    16261 tcgccgccgc tggtaaagtg ggttttcatc acttccggcc aggagccaaa tttgtcttcc
    16321 acgcggaaca gttcggtctg cgggaattta tctttcagtt tgtccatgac ttccgggtta
    16381 ttcacgcggt agtaatagtc ggtgatgatg gtttgcgcct gcgggctgta aagccagttc
    16441 agataggctt ttgctgcttt ttcggtaccg ttggcttgca cgtttttatc gacccacgcc
    16501 accgggaatt ccgccagaat gttggttttc ggaatcacca cttcaaagcc ctgcgcttcg
    16561 tactgtttgc ggatgttgtt cacttccgat tcaaagctaa tcagtacatc gcccaggccg
    16621 cgctcggcaa aggtggtggt cgcgccacga ccgccggtat cgaacacttc aacgtttttc
    16681 aggaactgag tcataaactg ttcggttttg gctttgtcgc caccgtcagc tttatccgct
    16741 gcgccccatg ccgccagata ggtataacgc gcgttacccg acgttttcgg gttcgggaaa
    16801 atcagcttca cgtcggagcg caccaggtcg ttccagtcgt ggatattctt cgggttgccc
    16861 ttacggacca ggaagcccat ggtggagtag aacggcgagc tgttattcgg caggcgcgac
    16921 tgccagtccg ccgggataag cttgccttta tcgtgcagaa tttgtacgtc ggtcacctgg
    16981 ttataagtga caacgtcggc ttttaagccc tgcaaaattg ccagcgcctg ttttgatgac
    17041 ccggcatgag attgttttat cgtcagtttg tcgccgccgt tatctttggc ccattgctgt
    17101 tcaaacggcg ggttcagggc ggcaaacagc tcgcgggaga cgtcataaga actgttcagc
    17161 agttccgttg cctgtacatg gcccgccagc agcagagaag cgaccagcgc gagtgagttc
    17221 tttttcagta agttaacggc cattgcgcac ccttataaat ttaatgactt tctaatagcc
    17281 atcatattta taacggacgt gaaaggagta acggttttat ataccgtttg gtgatttgga
    17341 agttgaaaag ggaataagac gaaatcctcc ccacaaatgc agggaggaaa cagaggtgaa
    17401 tcagataccg acgctaaccg tctccggcag tgtgctgccg ccatcaatca cattctgtgt
    17461 accggttaaa tagctggatt catccgatgc gaggaaggcc gccagttcgc cgacttccag
    17521 cggatcggcg aggcgacgca gcgggattgc ttttgccatt tcagtcagca ccgactctgg
    17581 atcttccggg ttcgactggc gggcaatgct ttccgccatt ggtgtgcgaa cgtatccagg
    17641 gcatatggcg ttaacgcgaa taccagactg cgcatactcc accgccagcg attttgtcag
    17701 gccaacaatc gccgctttcg ttaaggcgta cgccgtttca cccggatcgg ccaccatatc
    17761 accagtgact gaagacatca tcacaatgcg accatctttg cgggcaatca tctccggcag
    17821 caccgccttc gtgacgttcc atacgccttt aatattgatg tcaatatgga aatcgcgatc
    17881 gtcatcgctc atatcgagga aactgcccag acgacaaacg cctgcgttat tcaccaggat
    17941 atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct gcggctaccg acgccgggtc
    18001 acgcacatcg gcgacaaccg ccgtacagcg atgaccacga ccacacagtt cgtccgccag
    18061 cttttcgatc tcaggggaga tatccagcaa gattaggttt gcgccatgac gcgcaaaagt
    18121 tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg tcttgcccgt
    18181 gagtttaccc attttaatgc tctccttgtt aatacagagc gttacactcc attccctctg
    18241 aaaatacagg ataaacaaaa tattactgcg gatcagctca ctaattcagg aaaatctcaa
    18301 tccagtcgct gctttatgca tcatttcagg cgttgtgtta gctcactgct gcgctgaatc
    18361 attttcaatg actccacatc attgagttgc acaagaccaa caaacagtaa atcggtaaca
    18421 gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc accggaaacg
    18481 gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga agtaatggca
    18541 atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc ttttttactg
    18601 ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga aacggtggct
    18661 tgtacgtgcg tgtcggcttc gcaggccacg cgatatccaa ttttcatcag tttgaacgac
    18721 agatcgcacc ccaccagcgc cgatccgccc aggcccgtta tctggataaa cggtgcttta
    18781 ctgatgacct caatgatttt ttgcagccgc gcgtagtcga acaacgcaca ggtttgctcc
    18841 agcgccagct ctttttcgcg attcaatttg cgggcaatca cttccagcga atcatcactg
    18901 gtaatcgaac tgtgcaggtg ctgcgccgtg gcattcgttt tctcccggct ggcactgtat
    18961 tcgcctatta acgccatacg caattcagta aacccttgtg cgccgagctt ctgggcaaac
    19021 ttcacaatac tcgactggct gatgccgagc tgtttcgcca tcttgcgcga agagacggat
    19081 ttcagctcac tcacgttggc ccgcagaaag tcagcaattt tttgctcgtt ctcggtaaat
    19141 tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc tgggctggca
    19201 gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcgagtgt agactcaatg
    19261 attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat tatttgatcg
    19321 taaatagtaa ggtcaccacc gatgcaactt gaaaagatga ttaccgaagg ctcgaacgcc
    19381 gcctcggctg aaattgaccg cgtatcaacg ctggaaatgt gccggattat caacgatgaa
    19441 gataaaaccg taccgcttgc cgttgagcgc gtactgccgg atatcgccgc ggcaatcgat
    19501 gttatccacg cccaggtaag cggcggcggt cgtctgattt atctcggtgc gggaacatcc
    19561 ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt gaaaccgggt
    19621 ctggtagttg gattaattgc tggcggcgaa tatgccattc agcacgcggt ggaaggcgct
    19681 gaagatagcc gggaaggcgg tgttaatgat ttgaaaaaca tcggattaac cgcgcaggat
    19741 gtggtggttg gcattgctgc cagcggtcgc acgccgtatg tgattgccgg actggaatat
    19801 gcacgccagc tcggctgccg tacagtggga atttcctgta atccggggag cgccgtttcc
    19861 accaccgctg agtttgctat tacgccggtg gttggggctg aagtggtcac cggttcttcg
    19921 cggatgaaag caggcacggc gcagaaactg gtgctcaata tgctttccac cgggctgatg
    19981 attaaatccg gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac caacgaaaaa
    20041 ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc cgaacaagca
    20101 gaagcagcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat ggtgctgaaa
    20161 aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt tattcgtcag
    20221 gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct gaacaccatt
    20281 cttacccgtg tcggcggacc gggaaatatc gccagttgtg gtaactgtat gacgcgcctg
    20341 cgtctgggtg tacatgacag ttcactggtt gatcccgata tcaaaacgct ggaaggcgtg
    20401 aagggcgtca ttttgaccag tgaccaggtg caggtcgttt ttgggcctgg taaagcgcat
    20461 cgtgccgcaa aagcgatgag cgagctgctg ggagaagcac cggtacagga tgccgcagaa
    20521 atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt gcaacaattt
    20581 ctcgccaaat tcgccaccat cttcacgccg cttatccccg gttttattgc cgccggtctg
    20641 ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga tgctcagggc
    20701 acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt caccttcctg
    20761 gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt caatggcgca
    20821 attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg ttactacgcc
    20881 ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat cggcgtgttg
    20941 attgccgcgt gggcctgcgc ccgcatcgaa ggcatggtgc gccgctttat gcccgacgat
    21001 ctcgacatgc tgctgacctc gttaatcacc ctactgatca ccgctacgct ggcctatctg
    21061 atcattatgc cgctgggcgg ctggttattc gaaggtatgt cgtggctgtt tatgcacctg
    21121 aacagtaatc cgctcggttg tgcggtttta gccgggctgt tcctgatcgc cgtggtattt
    21181 ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcca aggattcaac
    21241 agcttatttc ccatcctttc gatggcaggc gcgggccagg tgggcgcggc gctggcactc
    21301 tactggcggg cgcaaccgca cagtgcatta cgcagtcagg tacgcggggc gattattccc
    21361 ggcctgctgg gcgttggcga accgttgatt tatggcgtca ccctgccccg catgaaaccg
    21421 tttattaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggatt gatagcctgg
    21481 tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt ggcgctgccg
    21541 ctgatgactt cagcacaagg cattcttccg gcgatggcag tctacgctgg cgggattctg
    21601 gtggcatggg tttgcgggtt tattttcacc acgctttttg gctgccgtaa cgtcaatctg
    21661 gactgatatc atgaaacgga caatgctcta tctttctctg ctggctgtta gttgtagcgt
    21721 cagcgccgca aaataccctg ttctgacaga aagttcgcca gagaaagcag ggttcaacgt
    21781 cgaacggctt aaccagatgg atcgctggat tagccagcaa gtggacgccg gttatcccgg
    21841 cgtaaacctg ctgattataa aagataatca gattgtgtat cgcaaagcct ggggagcggc
    21901 gaaaaagtac gatggcagcg tattgatggc gcagcctgta aaagctacca ccgggacgct
    21961 gtatgacctg gcctcaaaca ccaaaatgta tgccactaac ttcgctctgc aaaagctaat
    22021 gagcgaaggc aaactgcatc ctgacgatct gattgcgaaa tatatttcgg ggtttgccga
    22081 tagtccaaat gacaccatca aaggcaaaaa cacgctgcgg atttctgacc tgctgcatca
    22141 cagcggcggt ttcccggcgg acccacaata tccgaataaa gcggtcgcgg gcgcgttata
    22201 ttcccaggat aaaggccaga cgctggagat gatcaagcgc acgccgctag agtatcagcc
    22261 cggcagcaag catatttaca gtgatgtcga ttatatgctg cttggattta tcgtcgagtc
    22321 ggtcaccggt cagccgcttg accgctatgt tgaagagtcg atttatcgcc cgctcggcct
    22381 gacacatacg gtatttaacc cgctgctgaa aggctttaaa ccgcaacaaa ttgccgccac
    22441 ggaattgaac ggcaataccc gcgatggagt gatccacttc ccgaatatcc gcacctccac
    22501 tcactggggt caggtgcacg atgaaaaagc cttttattcg atgggcggcg tttccgggca
    22561 cgcaggtttg ttttccaata ccggcgatat tgcggtgtta atgcaaacga tgctgaacgg
    22621 cgggggctat ggcgatgtgc agctgttcag tgcggaaacg gtgaagatgt tcaccaccag
    22681 ctctaaggaa gatgccactt ttggcctcgg ctggcgcgtg aatggtaatg ccaccatgac
    22741 gccgacgttt ggcacgctgg caagcccgca aacctacggt cacactggct ggaccggaac
    22801 ggtgaccgtt atcgatccgg tgaatcatat ggcgattgtg atgttaagca acaagccaca
    22861 ttcgccggtt gccgatccgc aaaagaatcc caatatgttc gaaagcggtc agctgccgat
    22921 tgcaacgtat ggttgggtag ttgatcaggt gtatgcggcg ttaaagcaaa agtaataaaa
    22981 aggccagtca gtaatgttac tgcctggcct ttaatcaatg aatcagaaac gcttacagcg
    23041 ccatcaactt gtccagcgac ggtgcgaaat aatagccgcc ggttaccggt ttggtgaaac
    23101 gcaacatcgc atcacgctta ccatcggtat cgccaaacat gctcagcagt tgctgctcaa
    23161 tgttatgcag acgcgcgcag taggcgcaga agtacagacc gtgagtgcca ctggcggtgc
    23221 cgtacggcag gctctggcga acaatcttca gccctttgcc atcttctttc agatcaacgc
    23281 gggtcaggtg agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg gcctctttgg
    23341 tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg gttgagctgc ttcaggttgt
    23401 gctcccaacg ctgcacaaac acatagctgc cgccggcatc cacgccgtct ttgataaccg
    23461 ccacttcgcg acgcgtctct tcacccgccg ggttttctgt gccgtcaaca aagccgctca
    23521 ggtcacgctc ttccacccaa cggaagccgt ggatctcttc tttcacttca atgcagtcac
    23581 caaaggcttc catcgccgcc tgggcgacag agaagtttac gtcgtgacgc agagaaagaa
    23641 tgtggatcaa cacatcgaac tgggtggtcg gcgcaaggcc tttaccgtag cccggaaaat
    23701 ctttcagctc ttctgcccca acgccgccgc tcagagcgcg ccagatgttg ttaccaaagg
    23761 caaccaccgc accaagatgc gcgtccggga atttcgcttc aaaagttgcc agtttgtcgg
    23821 caaatgtttt actggccgca cgcagggcgt caacttcccc tttcacgttg gcttcgatcc
    23881 aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg aacctgagac attgttcctc
    23941 ctgaaaataa taatgccacg caatccgtgg cattgttgag cgctatttta cgtgtttttt
    24001 cgcggcggcg gtttgttcag acgcaaatta acgccgccag ataattttac tgactttcca
    24061 gtttttcagg gtatcgtccg aaggcattaa cccttcagga ccactccatt ccccgctgaa
    24121 ctggtagcta atatgttgac tgccttcggc tttacattcg acggcacgat tatcatcacc
    24181 atcagctttt tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg gtgtaccgat
    24241 tttaacaccg gtgtcagcag gaatatcgct atccagcacg tcaatgcgac tgatcgtgcc
    24301 ttgatcgccg ttaatcacca tcgccacgtt gtcgcccttc atcacttcaa aaaagcgcac
    24361 cacgttgccg ttcgcggttt tcattccgct gcgcaggcga taatcgccat caaccgcatc
    24421 ggcaatggct tgttcttgca gtggtgtgga cgccgttaat tcaccaacgc cctgctcgct
    24481 cactttggtg gacgacccaa accagttcca cgggttagcg gcagaccagt taactgacga
    24541 catcgtggaa cagccggtca gcatcaatgg catagcgcat aacattaaac gcagcgattt
    24601 catgtcactt cctttggtta ttcaataacg ttgcttggag tgcaaattca ccaaaaagtg
    24661 ccgttattct tttacttcat taaaacaagc gcgtaaacgt cgattggtca gcagccagat
    24721 taacgccaca atatccgcca ctaccagcgc cagaccgata ccgctaacgg attcaccgtt
    24781 cagccacagc cacggttgcc agcaaagtaa aaccacctgc gccaacagca acagaaaata
    24841 gagtacacgc caggtgcgag gaaacgtagc ccgccgaccg ctcaacaaaa acgccagcac
    24901 cgccggaatg ccaggaatca gccccagcca gaaattatcg tgatcgggat aaaacagatt
    24961 tagcagcgca gtaccctgct cgcgcgacgc accggcaatg acaaacagca cccaggttcg
    25021 cgcctgaagc aatagcacaa gccagaagag caagggtaaa cgcaggcgac cgtgcgcatc
    25081 ataatggaca ggatgaaact cagtactctt catcttcaat caaacgctta cccagactca
    25141 gcacgtcggc gtgttcatat cccaggcgtt catacattcc gagcaccatg tcgttatctt
    25201 ccggcacgtt gatctgaatt ttcgggcagc cgcgagcaat cagctttttc tccagccggt
    25261 taagcaacgc attggcaatc ccacgcccac gaaactctgg atgcacgcca agataataag
    25321 cagacccgcg atgcccgtca taaccgccca tcaccgttcc gaccacttca ccgttcacct
    25381 cagcgaccag aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc atttccggat
    25441 cgttccacgg acgcagcaag tcgcaacgct cccaaagggt gatgacctct tcgaaatctt
    25501 cctggcgaaa tacgcgtatc tccatggtat tcgttacctt tttgcgggtt aaaaggctga
    25561 ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa atcggttgaa aaagtggcat
    25621 aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacag caaacggtcg
    25681 taacggatta cgcgatacga tataacatct ggaactttat tattacaact caggccgtat
    25741 gagcactttt aaaccactaa aaacactcac ttcgcgccgc caggtgctga aagccggttt
    25801 ggctgccctg acgttgtcag gaatgtcgca agccatcgcc aaagacgaac ctttaaaaac
    25861 cagcaacgga cacagcaagc cgaaagctaa aaaatctggc ggcaaacgtg tcgttgttct
    25921 cgatccaggc cacggcggga ttgataccgg agcgatcggg cgcaacggtt cgaaagaaaa
    25981 acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc atgggattga
    26041 tgcgcgttta acgcgttctg gcgatacgtt tatcccactt tacgatcgcg ttgaaatcgc
    26101 ccataaacat ggcgcagatc tgtttatgtc aattcatgcc gatggcttta ccaacccgaa
    26161 agctgccggt gcttcggtat ttgccctctc taaccgtggg gcaagtagcg caatggcgaa
    26221 atacctgtct gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg cgactgacaa
    26281 ggatcatcta ttgcagcaag tgctgtttga tctggtgcaa accgatacta ttaaaaacag
    26341 cctgacgctc ggctcgcata ttctgaaaaa gattaagccg gtgcataaac tgcacagccg
    26401 caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt cggtgctggt
    26461 ggaaacctcg tttatcacca acccggaaga agagcgactg ttaggcacgg cggcgtttcg
    26521 tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt tatttccact ggttcgacaa
    26581 ccagaaagca cattcgaaaa agcgataagt tatgaaaccc gacgcacacc aggttaaaca
    26641 gtttctgctc aaccttcagg atacgatttg tcagcagctg accgccgtcg atggcgcaga
    26701 atttgtcgaa gatagctggc agcgcgaagg tggcggcggc gggcgtagtc gggtgttgcg
    26761 taatggtggt gttttcgaac aggcaggcgt caacttttct cacgtccacg gcgaggcgat
    26821 gcctgcttcc gccaccgctc atcgcccgga acttgccggg cgcagtttcg aggcgatggg
    26881 cgtttctctg gtagtgcatc cgcataatcc gtatgttccc accagccacg cgaatgtgcg
    26941 cttttttatt gccgaaaaac cgggtgcaga gccagtctgg tggtttggcg gcggcttcga
    27001 tttaacccct ttctatggtt ttgaagaaga cgccattcac tggcaccgca ccgcccgtga
    27061 cctgtgcctg ccatttggta aagacgttta tccccgttac aaaaagtggt gcgacgatta
    27121 cttctacctc aaacatcgca acgaacagcg cggtattggc gggctgttct ttgatgatct
    27181 aaacacgcca gatttcgacc actgttttgc ctttatgcag gcggtaggca aaggctacac
    27241 cgacgcttat ttaccaattg tagagcgacg taaagcgatg gcctacggcg agcgcgagcg
    27301 caattttcag ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct gggatcgcgg
    27361 cactctgttt ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt caatgccgcc
    27421 actggtacgc tgggagtatg attatcagcc aaaagatggc agcccagaag cggcgttaag
    27481 tgagtttatt aaggtcaggg attgggtgta actccctcac ccccattccc gcatccgctg
    27541 atgcaacgtc aacgacggct tctcggcaaa cagctgctgg taatccgtgg caaattgccc
    27601 cagatgccag aatccccact gcatggcggc gtcttttacc gttgtgcttt gcgaccacgg
    27661 gcttatcagt tcgcggcgta cggcgttcag gcgaatgcgt ttcagccacg cgttcgggcc
    27721 aattcctaaa atagcgtgaa acgcgttttg tagcgtgcgg cggctgacat gcagttgatt
    27781 acacaagtcc agcaccgtca ccggttcgga catgttttcc agcacatatt cacgggcgcg
    27841 ggaaagcaac cgacggtaac tctgatgact gatgctttcc gccgtcacca ttggctgcgc
    27901 ttcttccagc atcgccccca tcgccattag caaattatcc cccagcactt ttcgcactgc
    27961 aggctgatgg agattttccg ggttctcgca aaacgtcgcc agcgcctgtt ggacaaagcc
    28021 ccacagcgcg gctttatgct gctctttcac ttccagcgcc gactggctac gcaacatatg
    28081 taatacccga tccggattat gcaaaaagtt agcctgacgg gtgatgacat cttcagaaag
    28141 cactacgccc aggatcgtgt aatcgtccgg cgtacttaat tcaaactcgg ttccaccagg
    28201 gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg ataaatccct gctcaccgcg
    28261 cgtcgccgga atgccaaacc agaacgagtt cggccagacc agacacgact gacgcagcgc
    28321 cagaccggtg tattcacgaa aaacctgaat atcatcgagt agaatttccg taaattcacc
    28381 atgaaacttg cccggatgca gctgatcgta aatctgctgc caggcggtaa tcgttaaagc
    28441 atgttcatag acatccgttg tctgtcgttg atgaacatta tccacttcga ccttcggcgt
    28501 gagcttcagg ttttcgggta agggttcatg ataaagatgg tgcaaattgg ctgtacgggt
    28561 ctttttcatg atgttaatgc cgggtgttgt aggacacccg gcacctccga caggttaatg
    28621 gggcttgaga cgataacgac tactgcgttt acgtaacgtc ccagcagaaa agagctgttc
    28681 gagcgcattt ctggcttttt ccagcggcca gccaaagtgg gcagccactt ctcctgccgt
    28741 cactccctga cgtactgatg ttaacagcgc caacaactcg tcagcagatt ccgcgacgat
    28801 aactggcgcg tcgggttcct ttacgggttg cttcggctgg cggttaaagc cagtaaccag
    28861 ccactgggtg tcatcgtctg gccgaccgat ctccttacgg ctgatgacac gcccggtacg
    28921 catcgcggca gcacaacctg cgtccagcgc agcacgacac gccgccagat cgccttccac
    28981 caccagcgtt aagcgacctg ggtcaagcac ttcgtgactg agcagacgaa cgttagctgc
    29041 tttgagcatg gcatccgctg catctatcgc ggcgaccatg ccgtccactt ccagcaatcc
    29101 cagtgcattg atcattggca acctccgtta cgcacgctgg attggattac gcgcgatttc
    29161 cagcactgca tcggtaaagg cgttacaggc cgctttacac gctgcctggc taccggttaa
    29221 aaatgctgcc gagtagttag tttcagacgg tggcgggaca taggtcacca gctgtacgtc
    29281 ggcagatttc aacgctgcat caatgccgta ggtcgcttcc agcggcggtg ccaccagata
    29341 cgccatcgga tcgccaagcg tgatcccggc ggttgatgag agataagaac cggtacgcga
    29401 aactacatgt gccaggaatg ccgtatcttg cgcatcatta gcccactgga aagcagcgcc
    29461 attttcaata tgcgcaacca tcgcatccag accggcacgc acttccgccg ggtttggccc
    29521 accaagcata atcagcacct caccggcagt cggtgacggg ccgtgtgccg cgcccgcata
    29581 cagcgagcga ccatatacca cttctaccat cgcttgtttg gtcgcttcgt cagccgcaat
    29641 gtaggttacg tcgtcagaat cggcagaaat cagaccgagg ctacgaatat gtggcggcaa
    29701 tttcagttca cgcgcaaaat cggcgttaac ggaggcaatc acccgcatgg cggttaccga
    29761 cggtcgaatc aaatctaaag ctggcatgat gcctccttat cgggtcatgt tgatgccgga
    29821 tgctttctgc tccagcatac gtttcgccaa atccacaatg acggctgcgg cttcaaccgg
    29881 cggcgtgccg ccctggtgaa tgttagagat acaggtgcga tcggcctcaa cggtggtcgc
    29941 catacgcggc gagtaaacgg cgtagcagga gaggctttct gactgaccca accccggacg
    30001 ttcgcccacc agcaggatca ctactttcgc gccgaggatc tcaccgatct gatcttcaat
    30061 cttcacgcga ccataacgaa caaagaatgg cgtgccgact ttcagcccgg cctgtttcag
    30121 gcccgccatc agcggcggca ggatctcttc gtagttcaca gtgatggcat cagttgacag
    30181 gccatcagaa ataacgacct gtacgtccgg attagcaaca cactgtgctt tcagcgcttc
    30241 aacagcttct gcacacaggc ggcggcccat atccgggcga gtcaggtaga gatttttgtc
    30301 gctaatttcg gagcgtactt ccagcaagcc ctgcgctttc acccactctt ccggcacttc
    30361 tttcagtacg gtatctttcg aacgagagtg atcggccagg aaacgcagca gcgccagcgt
    30421 acgcggacgc ggaccggcac gaccggtaca aacgcgggcc acggtgctgc gacgcagttc
    30481 tgttaacaca tccgcacgat gtgggttttc aacaccaatc cacacttttg cttccgcaga
    30541 acctaaatcc agcgcgcagc tttccgaggt caccggtgcc gcacaggtgg tagtggcgca
    30601 ctttgcttct gacggcgcgg gggccgtttg tcccattgac gccatcacgc tgcgtacaat
    30661 ttcttcaatc tgtttttgat ccatgatgtg ttatctccgc gtcatcagaa gaacagtgac
    30721 ggatcgcccg cccgtttggt caggcgaccg tttgccataa tgcccatgct ttccagccag
    30781 cgttcaaact ccggtgacgg gcgcaggttg agtaactgac gcacagtggc ggtatcgtgg
    30841 aatgcggtgg tctggtagtt gagcatgatg tcatcaccca gcggcatccc catgatgtag
    30901 ttgcagcctg cggtggcgag caggatcatc aggttttcgt tgaggttctg gtcagcgtca
    30961 gcgtggttgg tgtaacagca gtcacagccc atagagatgc cgctcagctt gcccataaag
    31021 tgatcttcta agcccgcacg gataatctgg cggtcgttgt agagatactc cggcccaata
    31081 aagccgacca cggtgttgac gataaacgga tcgtaatgac gcgccagccc atagttacgt
    31141 gcttccatcg tcacctggtc tgcgccgaag ttagcgccag cggatagcgc tgaaccttgt
    31201 ccggtttcga agtagaggca gttttccccg gcgatacggt tgaactctgc acccactgcg
    31261 cgcgcttcgt cgagcatcgc cagttccacg ccaaactctt tcagcccttt ttcgctgcca
    31321 cagatactct ggaaaatcag tccgccaggc gcgccgcgac gaatcgcttc gatctgggtg
    31381 gtgacgtgcg ccagtacgca gccctgagtt gggatgttga atttgtcgat cacgccatag
    31441 atggtgtcca gcacgcggct taagttttcc acgtcgtcag tcaccgggtt aacgccgatc
    31501 accgcatcgc ccaccccgaa ggaaagccct tcgtagattt gcgcggcgat actttgcacg
    31561 tcgtcacggg tgtcatttgg ctgcaaacgg gcgctgaagg tgcccggaat accgatggtg
    31621 gtattggcct ttttgattac cggcattttc ttcgcgccgt agatcaggtc cgcgttggag
    31681 caaatcttcg ctaccgccgc gaccacttcc gaggtcagcc ctttgcgggt aaaggcaatg
    31741 tcgtccacgc tggtttcatc gctcagcaca tactcacgca gttcgctgat gctccagttt
    31801 ttaatctggt tgtaggccgt ttcgttaaca tcgtcctgaa tcagccgcgt cacgcagtca
    31861 tcttcatagg caatcaccgg attattgcgg atgtccgcta cggtcatttc cgacaacacc
    31921 tgctttgccg ccacgcgctc ctgtgagctt gccgctgcga cgcccgccag cacatccccc
    31981 gaacgcagtt cgttggcttt agccagcacc tcttttacat ccttaaactg atatacattg
    32041 ccgaacaatg tggtctttag tttcataagt cgttccctca ggaaggaaat gcgagtgatt
    32101 tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt accaatgtcg atatagtccc
    32161 ccgcgcggac aatcacttcg tcaatgactg ccaacgggag ttgttgtagc tgtgggcgca
    32221 acagcatgcc cagagcttta ccaaagtcct gcccggccac caccagcagg ggatgcggat
    32281 tcggaaaacg cgcgacgaaa tcgaccaacg cgttgatgac cgtcagtacc gcggcgtaac
    32341 gcacaggcag cgaggcggga agcgccagca cgtacgcgtc agttttggga tcaagatcca
    32401 gctgaatcag cgcctgttgc caggcactca ccagatccgt ttcatcaatc gggatcgcca
    32461 ccggcaaatt gcgcagcggc agttgtacgc cctccagcca gattgtgctg ccagagagcg
    32521 aaagggtatg tgcgcccgcg ccaatcaccg tggcgcgcac ggtttgcgcc ggaaactgca
    32581 cgttcatctc acgcaggcgc ggatggtcat gtagcgccgt cgccagtagc gggccaatat
    32641 cggcaaaaca gaacgggtca gcgggctggt ggcgataaca ttcgcccacg ccgccagaaa
    32701 gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatt aatgcctgcg
    32761 cgagcggcga aagcgttccg tcaatcactt cgacaatcag ctctgccatc cgtcgggtaa
    32821 cctgcaccag ctgcgcgccg gtcagcgaac ggacgtcagt gcctgcaccg aaacactcat
    32881 ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg cccctggctg tcggtttcca
    32941 gcaggcgacc accgacgttg aggcaggcag tgccgctgat ttttccggca tcgaacaggg
    33001 cgtagttcgc ggtgccaccg ccgatgtcga tattcagtac ccgacacagc cgttgttcag
    33061 aaagggtttg cgccccggca ccgtgaccgg cgatcacgga ttcgaggtgc ggcccggcgc
    33121 tggcgacgac aaaatcgccc agcgactgag agagcgccat caccgccggg cgagcattgc
    33181 gggttttcgc gctttcaccg gtgatgatga tggcaccaga atcaacgctt tccggcgcaa
    33241 tgcccgcagc ctgatattgc tctaaaatca gcgatttcag ttccgcttct tttaaaccac
    33301 cctgtttatc gacaggggta aagaacaccg ggctttgcca gctaatttcg cgtttgatga
    33361 attcgtagcg cggcacctgc gacaccgccg cacggttaac cagttccagg tgggagaaaa
    33421 tcacctgggt ggtggtggtg ccgatatcga taccgacgct cagtagctgg cgagtgttca
    33481 cgattgtgcc tccgcttcgg ttttagccgc ggtcgcgtcg tcttttggca ccagcatcat
    33541 cgccacgcca atcgccgtta cgccgccgat caacttgccg acaatcatcg ggaagatcat
    33601 ggcattcatg ttggcagcgg cgaagcctaa gtggtcgccc agggcgaaag cagcggaaac
    33661 ggcgaaggcg cagttgatga ctttgccgcg ggtatccatc tgcttcatca tgccgaacat
    33721 cgggatgttg ttggcaagcg ttgccaccat gccggctgcc gcgatgttgt tcatattcag
    33781 cactttaccg acgctcatca gcggtttttc aaaccagcgg gtcagcagca gcaccatcgg
    33841 atacgcccct aacagaacgc aggagataga accgataact tcaatggcgc gcatcacctc
    33901 accgggttta tcgccagggg ccataaagat aggatccaga ccggggatca gttcccagcc
    33961 aagcaggaat ttcactaccg cagcggcaag accgagggtg atcaatgcaa cgaggaattt
    34021 ggcgaagatc tggaagccgt tgatcatttt ttctgggatg aatttcagcc ccagcgccac
    34081 cagaatcgca acaatgatca ccgggatcat gttcatcagg atcagggcga aagtgaattc
    34141 caccggctgg ccgttgatct gaacaccgga gtacatagca accagaccac cagcgataca
    34201 accaatcgga atggtcacaa tgcccgccag cacgccgagc gccagataac gacggtcaga
    34261 aggttcgata atgccgagcg ccaccggaat ggaaaacaca atcgttggcc ccatcatcga
    34321 cccgagaatt aacccagagt atagccacgc ggctacgtcg ccgcccgcca gctctttggc
    34381 gaggaagaag ccgcccatat cgcacgccag cagtgttccg gcgaacatcg atgggttagc
    34441 gccgagcatt tcgtaaaccg gaataattac cggcccgagt acgtgagcca gtaccggtgc
    34501 cagcgcggtc ataccgacca tcgccaggcc cagtgcgccc attgccataa agccttcttc
    34561 gaactgaccg ccggatcctt cgatactttt accgaactta ccgaggaaac gagcagaacc
    34621 gccgaactgt gacaggatcc tgtcaacggc agctatcagc ataaagaaca tcatgatgta
    34681 catgatgatt tcgttaattc ccatcgcctt tactccctgt tagttgttat ttattagcgg
    34741 atgctgcgtg aagccttatc cgcactacat gtgcaatccc acagaccgga taagacgcgg
    34801 catttattgc gccgctgcgt acaggccgac aagctgttcc aggctggcgg tacgcgggtt
    34861 gctgcgcaga caaatatctt ccagcgcggc ctgcgcccat gcaccgtaat gcgcagatgt
    34921 cgcgccaaca tcgcccagtc gtttaccaat cccaacttcc gcaatcagct cacttaccgc
    34981 gttaatagcg tcacgatcgt cggatttttt agttcgcagt gcccgaccaa tctgactaaa
    35041 gcgttcgcga caaaccatcc ggttaaactc catcaccgtt ggcagcaaca tagcgttcgc
    35101 gagaccgtgc ggaatatgca gcgccgcgcc cggctgatgc gccatcgcgt ggcacaaccc
    35161 aagacccgca ctggaaaacg ccattcccgc catacatgaa gccagcaaca tgctctcgcg
    35221 cgcggcaagg tcgtgaccgt agcccaccgc tttcggcagc gatttgccaa tcatcgcaat
    35281 ggcaccaatc gccagactgt cggtaaacgg tgtagcgttc agggcgctgt atgcttcaat
    35341 ggcatgggtt aacgcatcaa tgccggtcat cgccgtgaca tgcgacggca caccttcggt
    35401 caatgcggcg tcgaggatcg ccacatccgg catcagcgag gcatgggcta acacctgctt
    35461 gcgcccgctc accgcgtcga taatcaccgt tacattggtg gtttcagagc cggttccggc
    35521 ggtagttgga atggcaatca gcggcaagcg cggttgcaga acgctggttt ctgacatctc
    35581 tgccagcgtg ctatccgggt tcgtcaccag caacgccacg gctttcgccg catccagcac
    35641 cgagccgccg ccaaatgcga tcaccccgtc acaacctgac tcacgcaact gcgccacggc
    35701 tgcacacacg tcggtaatgc acggttcgcc caccggacat ggccagagcg tcatggcgat
    35761 acctttaacg gccaggctac gcgtcagccc ggcggtcatc cctgcctgat gcaaaaagct
    35821 gtctgccatc acgaacagat gtttcagccc acgcgtttgc gcttgctgtc cgcaactgct
    35881 caccgagccc ggaccgcaaa gcgtcaccgg tggaacgcta aatgttttta cccgttgcag
    35941 attcagggta tcgaacgcct gaaagagcgc ggtctgcaat tcattttgca tatagcccct
    36001 ccgctttctc tcgcccacta ctggcgatcg ccagcggggt cataaacaaa ctgtgctgcg
    36061 gtaaatgcac ctgtaacgcc gggaattgtt tgcgaaacag ttctgccacg cccggttgca
    36121 tacaggagcc gcccgccagc cataaatcag taatgccctg cccttcaata tgccgggcga
    36181 caatgtccgc cattttttcg tacaccggtt tcaccgtagg ccaaatctct tcaccgtgac
    36241 cgcgcttgta ctgctccgcc tcttccagcg aaatacggcg atttccggcg aaagtcagag
    36301 agatgtgatg tccgccggtg gcttcatccg ccgagtacgt cactttgccc tttttcacga
    36361 tggcaatgcc ggtagtgcca ccgccgatat ccactacacc ggcgttgtcc agttgcagca
    36421 gatccgcgac tgcggttggc tcatccagca catggctcac ttccagcccg gcagactcca
    36481 gcacgttgat ggaaatacgc gggtccgtac ctggaggaaa tgaagtcgcc gcatggctaa
    36541 aacgacgacc gaattgctgc tcgagcgtgt cgagatggcg acgaacaatg gtgaccgcgc
    36601 cgaagaaatc ccagacgatg ccgtcgcgaa ccacatcggc ccagtcgagg cataccgcca
    36661 ctggctgacc gtcgcggtcg acaaccatcg acaccacatc gcaggtgccc agatccactc
    36721 ccagccacag cggagattcc gtcgcggcgg gcgtctggtt acacagcgtt gccgccgttt
    36781 gcagacgtgg ggtgagccat tgttcgtcgt gcgccatctg ttactcctta aacaatgcga
    36841 aacgcatcga ctaatacaca gcgacgcaga cggacaaacg tacgcgcgct ggtcacccct
    36901 tcaccggttg gcgtggtgat ggtcatggtg gtccagcctt ccccgcccag ccccagcccg
    36961 gcaatgcacg gtccgttctt aacgaaaatg ctggtatcaa tagcattcgc catctggttc
    37021 atgttttcga tgttgcgcga gtgcattgcc gccgtgtggt ggcaaccgcc ttccagtttc
    37081 accgctagcg caatggcatc cgccacgttg gcgacgcgca cgacgggcaa caccggcatc
    37141 atcagttcag tcacggcaaa cggatgttct gcggtggttt ccacaaacag caggcgcgtt
    37201 tcttgcggaa ctttaaggcc gattgccgcc gcgattttgc ctgcgtcgcg accaacccag
    37261 tcacggctga cggtgccttt tccgcgctcg tcgatatttt tcagcaacac cggttgcagc
    37321 tgctgcgcct gttctgcggt cagtttcacc gcgtgctggc cttccatcag acgcatcagt
    37381 tcatcggcta cgctatcaac aacaatcagt accttttcgt cggcacaaat gatgttgtta
    37441 tcgaaagacg cgcctttgac gatggactga gcagcacggg cgaggtcagc ggtttcatcc
    37501 accactaccg gcgggttgcc agcgcctgcg gcaatcagac gtttattggt gtgtttacgc
    37561 gccgcttcta ctaccgcttc gccgccggtc accaccagca ggccgatacc cggaaacttg
    37621 aacaagcgtt gcgcggtttc gatatccgga tttgccacag taaccagtaa gttttccggc
    37681 ccgcctgcgg caacaatcgc ctggttgagc agcgtgatcg cccgctggga gacttttttc
    37741 gccgccggat gcggggcaaa aatgacgctg ttgcccgcgg caatcaggct gatggcgtta
    37801 ttaattacgg ttgccgccgg gttagtggac ggcgtcaccg aagccaccac gccccagggc
    37861 gcgttttcaa ttaaagtcag gccgttgtcg cccgtcagca cttgcggaga gagacactca
    37921 acgcctggcg tgccgcgtgc ctgagcgacg tttttggcaa atttatcttc aacgcgaccc
    37981 atgccggttt cactgacggc aagttccgct aaatctctgg cgtgtttttc gcctgcttca
    38041 cgaatggcag caatggctaa ctggcgcatt gccacgctct ttaacccttg ctgggcaatt
    38101 ttggctgccg caacagcgtc atccagggac gcgaaaacgc ccatctcatg aacggcggcg
    38161 ggcggcgtgt cactgctttg cattttcagc agtaccgctt tcaccacctg ttcaatatcc
    38221 tgttgattca tgatgttctg ccttatttgt ggaaaattac ctgaccgcca gacaccacct
    38281 catcgacgat gccaatcacg cacagatcga ccggtgacgt ttcgctttta tgcgcctggc
    38341 gggcggaact gccactcacc agcaacaccc actccccggt tcccgcgcca atattgtcga
    38401 tggcgacggc acattgcccg tcaggattac catgtggatc aatcatttcc accatcagca
    38461 atttgtcatg cgccagtccg tgatggcgta cggtacaaac aatttgtcca gtgacgactg
    38521 ccagtttcat acccgcctcc gtggcgtatt tcaggtaaaa gctcccccta ccctccgcgg
    38581 aaggtaaaat gaaaaatgag agagcgtgac gcccgaatcg acgtcacaca gggtgattac
    38641 aggttgctgc tatcgccttt caggccgatc gggaacactt cttccaggtc gccgtgcgga
    38701 cgcgggataa cgtgtacaga taccagctcg ccgatacgct gtgccgctgc ggcaccggca
    38761 tcggtcgccg ctttacaggc tgcaacatca ccacgaacca tggcagtaca caggccgccg
    38821 ccaatctgct taacaccaac cagcttgacg cgcgcagctt tcaccatcgc gtcagaagcc
    38881 tcaatcagtg caaccaggcc ccgggtttcg atcattccta atgcttccat tgtgctttcc
    38941 tctttatcag ggtccagaac gggaccgttc attcaaccag tgtttgtaaa ctgctttcgc
    39001 ggttcacttc tgtctgacgc ggcacagctg ccaccagcgc cagctcgata atttcctgca
    39061 cactacaacc acgagagaga tcgtgcatcg gcgcggcaag tccttgtatc aatggcccga
    39121 cggcacgata tccgccgagt cgttgtgcga ttttgtagcc aatatttccg gcttccagcg
    39181 acggaaaaac catcacattg gccttgccct gtagcgggct ggcaggcgct ttttgcgccg
    39241 ccacttccgg cacgaaggcg gcgtcaaact gcaactcgcc atcgactacc agctttggtg
    39301 cgcgctcacg gacgatttct gtcgcctgct ggacattggc aacgcagggg tgacgggcgc
    39361 taccgttact ggaaaacgac agcatcgcca cgcgcggctc ttctccggtg atggcgcgcc
    39421 aggtttcggc gctggcaagc gcgatatccg ccagctgcgc cgccgtcggc tgtggtacca
    39481 cgctgcaatc ggcaaagccc aacgccggac cgctgtactg tggcagcatc aggaaaatgg
    39541 atgagagcgt tttacagcct ggctgcaagc cgataatgcg taatccggca cgcagcacat
    39601 tcgccgtgga agagaggttg cccgcgatac agacatccgc tttaccggcg ctgaccattg
    39661 cggcggcgaa catcagcgga tcggtaagtt tttccagcgc atccggcggc gttttttcgc
    39721 ccgcgcgggc cagccagcga tgagcaaatt cttcccgcat tgcgaggttg ccatgcggat
    39781 ctatcacctg tagcccgtcc atcgccacgc cgtgactgag cgcaaactga cgaagttcaa
    39841 acggattggc gaccagaatg ggcgttgcca gaccttgttg atgtaaatat tgcgcagctt
    39901 tcagcacacg ttgatctaac gcatccggaa aaaccactct ggcgggcgct cgcaacgcca
    39961 gttcacaaca acgttcaata atcattgcgt ttcccccagt cgttgttgga tctgcttcac
    40021 cgttagcggc tgcttcgtta cgctcaggat catcatcacg tagaccgtgc tggaaaggcg
    40081 gttcagcgcc tgcaagatgt ccgggcgtaa tacttcaaaa ctgcgggtga taaacacctg
    40141 cgccgctacg gtttcggctt cccgcacttt ggtgcgtagc agattcagca acgccgcatc
    40201 gcgcccgtgg cttgcttccg gcaccagatg atcgtgacca agataacgca acggctgatg
    40261 agaaagccgg tgcagatctt cgtcagaaag cccgacaatc gcctggcagc ccagcggttc
    40321 cgccagttca atttgcagcc acactgccag cgcaatggtg ctgtcgagaa ctgcgcgaaa
    40381 acccagacgc ggatcgcttt tggcgaccat tttttccgcc gacaggtgag tcagcgtatc
    40441 cggctttttt gccaccggct ggcggcacag ttcgcagcac gcctgcggat gttcatcgct
    40501 actggttagc ccatgaacgg gctgcggctg ctgctgttca tcgtcaacaa acaggcggcc
    40561 ctgctcgtca ataaacttga tgcgcagatg gcggctttcc agcaactccc gggcagaggg
    40621 cgtcaggcga ctgtccgcag ggagatggat ctctgcgcct tcgctgagcg tatggttcgc
    40681 tcttagccat gcttcggtga tgaaatcttt catagggatt gccagttagc cggccaggcg
    40741 acatacagga atttcacgct ggatgtcgta ccaaattcga tgctggagcc tttcgggata
    40801 aacatcacgt cgcctgcttt agcgatcatg gtttcgcctt cgtggcgaac atggagctcc
    40861 ccttccagca ccatgtcgat ttcgtcgtag ttcagggtcc acgggaagaa tgcgttctcc
    40921 cactgcataa aaccggcggc catgctgctg ccgtcgtctc cggtcacaag atcagtcaag
    40981 cccacgcaat gcggttgtgc gccatcaaag cgaccaaact tgacgctgct accatcaatc
    41041 actttgatgc cgcctttgcc ggtcaccgat ttgaagctcg gctgcattgc accctgctcc
    41101 agcgactgtt tttccttcat cactttttcc atcagctgcg cgaccaggct ttcggtaaac
    41161 tggccttccg gtagctgggc gataatggtt tcgcggatgc gctggctttc ggttttatcg
    41221 gctggcacgc tggcgggcac ggatgccgtt accgggatca attcatcaca ttcggtgatg
    41281 gtaaaaccca gtagatccgc cacttcacgg gcttccgggg taataatgct ggcgcgcaga
    41341 accacggaca ttgcctgttc gccgcgcgcg tgtgcttcac gaatatcatt cgctgtgatg
    41401 agttttttca cctgcttcct cctgtttggt gagagatgcc agataatcca ccagttgctg
    41461 cacgctttgc gggtcgtggc tgtttagctc gaaaatcggc tcctcgaagc ccgtctcaag
    41521 caataacttt cgcgttgcgg cgacgtcagc atccggcatg tccgttttac tgatgacggc
    41581 gatttgccgt ttgctgacgc caatatccag caacccggca ggcaaccgac tttccgggtc
    41641 attcgcgccg tggacataga tcagcatgtc cacatcctgt agcgtggtaa ttaaggcgtg
    41701 ataccagcgg ggatggctaa aatattcacc cggcgtatca atatcgccct tatcattaaa
    41761 ttccacggcc tgtgtttttc tggcgagggt ataattcccc tgcaacgcat taaataacgt
    41821 tgttttccct gccccgacgg tacccacaaa cgcaattcgt ttcatgaatg cctcgtatta
    41881 acttttggtc atttcacaaa gcgtataatt taataagcga cctaaaccac tgacggtttg
    41941 tgataacgct tcttctactg cgcccaccga gccataaatc accaatgcac cactgaagcg
    42001 gtcgagaaat ccgatatgca catcggcggc tttcagcgct aaatcaccgg cgatcatcgc
    42061 cgtttcgccg ggagttagcg tcataatgcc aattgcgccc gcatccggaa cgccgatttt
    42121 cttcgccagt tcctcgccgg gatgcgcgat gagatgcgcc agtgtgacct gtttgccggg
    42181 cacaaattcc tgaatgatgc gttctttatc catgagtcgc cgcctgtgaa aaactttcgt
    42241 gtatcgtgac aatttttcgt cagagtaaat aacaaaattc ggcaagcgcg tttaaaaggt
    42301 gaggtagatc actaagaatg gagagatatt tactgttatg aataaacttt catatgcgac
    42361 gcatgaatat aaataa
//
LOCUS       DAZKIF010000024        41530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZKIF010000024 DAZKIF010000000
VERSION     DAZKIF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.024"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..303)
                     /locus_tag="ACHOTW_001366"
     CDS             complement(<1..303)
                     /locus_tag="ACHOTW_001366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843506.1"
                     /translation="MGKNRQSAAAARCCRSEVIFLHLLSKASLALAFFFYLTAVDRRS
                     RRIRQKNRLHNNIILPDHVSPQIIITTETDYTNHNRQRFVIFPYYYSVFTVVTFLFS"
     gene            complement(249..1403)
                     /gene="metK"
                     /locus_tag="ACHOTW_001367"
     CDS             complement(249..1403)
                     /gene="metK"
                     /locus_tag="ACHOTW_001367"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9843507.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            1459..1710
                     /gene="yqgD"
                     /locus_tag="ACHOTW_001368"
     CDS             1459..1710
                     /gene="yqgD"
                     /locus_tag="ACHOTW_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP9843508.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            1821..1964
                     /gene="yqgG"
                     /locus_tag="ACHOTW_001369"
     CDS             1821..1964
                     /gene="yqgG"
                     /locus_tag="ACHOTW_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP9843509.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            2058..2189
                     /gene="yqgB"
                     /locus_tag="ACHOTW_001370"
     CDS             2058..2189
                     /gene="yqgB"
                     /locus_tag="ACHOTW_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP9843510.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2198..4174
                     /gene="speA"
                     /locus_tag="ACHOTW_001371"
     CDS             2198..4174
                     /gene="speA"
                     /locus_tag="ACHOTW_001371"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP9843511.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            4320..5051
                     /locus_tag="ACHOTW_001372"
     CDS             4320..5051
                     /locus_tag="ACHOTW_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9843512.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            5187..6107
                     /gene="speB"
                     /locus_tag="ACHOTW_001373"
     CDS             5187..6107
                     /gene="speB"
                     /locus_tag="ACHOTW_001373"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP9843513.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6313..7071)
                     /gene="loiP"
                     /locus_tag="ACHOTW_001374"
                     /gene_synonym="yggG"
     CDS             complement(6313..7071)
                     /gene="loiP"
                     /locus_tag="ACHOTW_001374"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP9843514.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            7349..9340
                     /gene="tkt"
                     /locus_tag="ACHOTW_001375"
     CDS             7349..9340
                     /gene="tkt"
                     /locus_tag="ACHOTW_001375"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9843515.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            9654..10097
                     /gene="cmtB"
                     /locus_tag="ACHOTW_001376"
     CDS             9654..10097
                     /gene="cmtB"
                     /locus_tag="ACHOTW_001376"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP9843516.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            10125..11513
                     /gene="cmtA"
                     /locus_tag="ACHOTW_001377"
     CDS             10125..11513
                     /gene="cmtA"
                     /locus_tag="ACHOTW_001377"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP9843517.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            11528..12805
                     /gene="yggP"
                     /locus_tag="ACHOTW_001378"
     CDS             11528..12805
                     /gene="yggP"
                     /locus_tag="ACHOTW_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9843518.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            12802..13767
                     /gene="glpX"
                     /locus_tag="ACHOTW_001379"
     CDS             12802..13767
                     /gene="glpX"
                     /locus_tag="ACHOTW_001379"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9843519.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            13789..14298
                     /gene="fumE"
                     /locus_tag="ACHOTW_001380"
     CDS             13789..14298
                     /gene="fumE"
                     /locus_tag="ACHOTW_001380"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP9843520.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            14295..15008
                     /locus_tag="ACHOTW_001381"
     CDS             14295..15008
                     /locus_tag="ACHOTW_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP9843521.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(14980..15657)
                     /locus_tag="ACHOTW_001382"
     CDS             complement(14980..15657)
                     /locus_tag="ACHOTW_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9843522.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(15651..16328)
                     /locus_tag="ACHOTW_001383"
     CDS             complement(15651..16328)
                     /locus_tag="ACHOTW_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9843523.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(16316..17023)
                     /locus_tag="ACHOTW_001384"
     CDS             complement(16316..17023)
                     /locus_tag="ACHOTW_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP9843524.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(17024..17602)
                     /locus_tag="ACHOTW_001385"
     CDS             complement(17024..17602)
                     /locus_tag="ACHOTW_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9843525.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(17625..18056)
                     /locus_tag="ACHOTW_001386"
     CDS             complement(17625..18056)
                     /locus_tag="ACHOTW_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFP9843526.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            18428..19447
                     /gene="epd"
                     /locus_tag="ACHOTW_001387"
     CDS             18428..19447
                     /gene="epd"
                     /locus_tag="ACHOTW_001387"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP9843527.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            19497..20660
                     /gene="pgk"
                     /locus_tag="ACHOTW_001388"
     CDS             19497..20660
                     /gene="pgk"
                     /locus_tag="ACHOTW_001388"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9843528.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            20875..21954
                     /gene="fbaA"
                     /locus_tag="ACHOTW_001389"
     CDS             20875..21954
                     /gene="fbaA"
                     /locus_tag="ACHOTW_001389"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9843529.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(22018..22116)
                     /locus_tag="ACHOTW_001390"
                     /pseudo
     CDS             complement(22018..22116)
                     /locus_tag="ACHOTW_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22312..23172
                     /gene="mscS"
                     /locus_tag="ACHOTW_001391"
     CDS             22312..23172
                     /gene="mscS"
                     /locus_tag="ACHOTW_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP9843530.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            23311..23946
                     /gene="argO"
                     /locus_tag="ACHOTW_001392"
     CDS             23311..23946
                     /gene="argO"
                     /locus_tag="ACHOTW_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP9843531.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            24039..24779
                     /gene="yggE"
                     /locus_tag="ACHOTW_001393"
     CDS             24039..24779
                     /gene="yggE"
                     /locus_tag="ACHOTW_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP9843532.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            24946..25842
                     /gene="ygfI"
                     /locus_tag="ACHOTW_001394"
                     /pseudo
     CDS             24946..25842
                     /gene="ygfI"
                     /locus_tag="ACHOTW_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(25839..27317)
                     /gene="scpC"
                     /locus_tag="ACHOTW_001395"
     CDS             complement(25839..27317)
                     /gene="scpC"
                     /locus_tag="ACHOTW_001395"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP9843533.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(27341..28126)
                     /gene="scpB"
                     /locus_tag="ACHOTW_001396"
     CDS             complement(27341..28126)
                     /gene="scpB"
                     /locus_tag="ACHOTW_001396"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP9843534.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(28137..29132)
                     /gene="meaB"
                     /locus_tag="ACHOTW_001397"
     CDS             complement(28137..29132)
                     /gene="meaB"
                     /locus_tag="ACHOTW_001397"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP9843535.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(29125..31269)
                     /gene="scpA"
                     /locus_tag="ACHOTW_001398"
     CDS             complement(29125..31269)
                     /gene="scpA"
                     /locus_tag="ACHOTW_001398"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFP9843536.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(31473..32366)
                     /gene="argP"
                     /locus_tag="ACHOTW_001399"
     CDS             complement(31473..32366)
                     /gene="argP"
                     /locus_tag="ACHOTW_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP9843537.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            32749..33408
                     /gene="rpiA"
                     /locus_tag="ACHOTW_001400"
     CDS             32749..33408
                     /gene="rpiA"
                     /locus_tag="ACHOTW_001400"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9843538.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            33663..34895
                     /gene="serA"
                     /locus_tag="ACHOTW_001401"
     CDS             33663..34895
                     /gene="serA"
                     /locus_tag="ACHOTW_001401"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9843539.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            35124..35183
                     /gene="ibsC"
                     /locus_tag="ACHOTW_001402"
     CDS             35124..35183
                     /gene="ibsC"
                     /locus_tag="ACHOTW_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP9843540.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            35517..35576
                     /locus_tag="ACHOTW_001403"
     CDS             35517..35576
                     /locus_tag="ACHOTW_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9843541.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(35677..36279)
                     /gene="fau"
                     /locus_tag="ACHOTW_001404"
     CDS             complement(35677..36279)
                     /gene="fau"
                     /locus_tag="ACHOTW_001404"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9843542.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(36300..36483)
                     /gene="ssrS"
                     /locus_tag="ACHOTW_001405"
     ncRNA           complement(36300..36483)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHOTW_001405"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(36525..36854)
                     /gene="zapA"
                     /locus_tag="ACHOTW_001406"
     CDS             complement(36525..36854)
                     /gene="zapA"
                     /locus_tag="ACHOTW_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9843543.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            37022..37600
                     /gene="ygfB"
                     /locus_tag="ACHOTW_001407"
     CDS             37022..37600
                     /gene="ygfB"
                     /locus_tag="ACHOTW_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP9843544.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            37626..38951
                     /gene="pepP"
                     /locus_tag="ACHOTW_001408"
     CDS             37626..38951
                     /gene="pepP"
                     /locus_tag="ACHOTW_001408"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP9843545.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            38948..40126
                     /gene="ubiH"
                     /locus_tag="ACHOTW_001409"
                     /gene_synonym="visB"
     CDS             38948..40126
                     /gene="ubiH"
                     /locus_tag="ACHOTW_001409"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP9843546.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            40150..41352
                     /gene="ubiI"
                     /locus_tag="ACHOTW_001410"
                     /gene_synonym="visC"
     CDS             40150..41352
                     /gene="ubiI"
                     /locus_tag="ACHOTW_001410"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP9843547.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(41426..41509)
                     /locus_tag="ACHOTW_001411"
     CDS             complement(41426..41509)
                     /locus_tag="ACHOTW_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843548.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
BASE COUNT         9879 a        10319 c        11142 g        10190 t
ORIGIN      
        1 actgaaaaga aatgtaacaa ctgtgaaaac cgaataataa tatggaaata ttacaaatct
       61 ttgtctgttg tggttggtgt aatcggtttc agttgtgatg ataatttgcg gtgaaacgtg
      121 atcaggaaga atgatgttat tgtgcaggcg gtttttttgc cggatgcggc gtgaacgcct
      181 atccaccgcg gtgaggtaaa agaaaaaggc cagcgcgagg ctggcctttg aaagcaggtg
      241 aagaaagatt acttcagacc ggcagcatcg cgcagcagct gcgctttgtc ggttttttcc
      301 cacgggaaat gttcacgacc aaagtgaccg tatgctgcgg tttctttgta gatcgggtgc
      361 agcagatcca gcatctgaat cagaccgtat gggcgcaggt cgaagaactc acgtaccagc
      421 agagtcagtt gttcagaagg cactttctca gtaccgaaag tttctaccat gatggaagtc
      481 ggttcagcca cgccgattgc gtaggaaacc tgaatttcac aacgatcggc caggccagca
      541 gcaacgatgt ttttcgcgac ataacgtgct gcgtaggctg cggaacggtc cacttttgat
      601 ggatctttac cagagaatgc accgccaccg tgacgcgcca tgccgccgta ggtatcaacg
      661 ataattttac gaccagtcag accgcagtca cccatcgggc caccgataac aaaacgaccg
      721 gtcgggttga tgaagaattt ggtggcagaa gtcagccatt cagcgggcag aatcggcttg
      781 atgatctctt ccattaccgc ttcttgcagc gatttctggt cgatctcttc agagtgctga
      841 gtggaaagca cgacagcatc gataccaacg attttgccgt cgtcatactg gaaagtcacc
      901 tggcttttcg cgtccgggcg cagccacggc agagtgccgt ttttacgcac ttcagcctga
      961 cgctgtacca gacggtgtgc ataggtgata ggtgctggca tcagcacgtc ggtttcatta
     1021 gttgcgtagc caaacatcag accctggtca cccgcgccct gttccagcgg atcggcacgg
     1081 tcaacgccct ggttgatgtc aggagactgt ttgccgatag cgctcagaac tgcacaggag
     1141 ttagcgtcaa agcccatgtc ggaatgcaca tagccaattt cgcgaacggt gttacgggtg
     1201 atctcttcga tgtctaccca ggcgctggtg gtgatttcgc cgccaactaa aaccatgccg
     1261 gtttttacgt aggtttcgca agcaacgcgt gctttcggat cctgttcgag gatcgcgtct
     1321 aaaacggcat cagagatttg gtcagcaatt ttgtcaggat gcccttcaga gacggactcg
     1381 gacgtaaaaa ggtgttttgc catatttaat atcacctaaa gagaatttgg ttagctcaaa
     1441 ctgttgtgtg gattttctgt ggtagcggat cctaccacga ctctgcaggt taaaaacact
     1501 ggcagtctga gtgttaatcg gtatggatgg attaacatct ggatggctat tttaggtcaa
     1561 ttcttcaccc gatttccact tttttttgaa tcgtgtctca ttctgttaaa aacgtggctg
     1621 gaaatttttc ctgacaatgc cggcattctg cgtatttatc ttttgcaatt ttctgccatt
     1681 gtggggtata aaacgcggcg cgcggcttaa ataaaaagca cacgacgttt ctttcgtgtt
     1741 gccacttcca gccgggttga aatcagagtt ttggcttgtg ggttcgtctt aacaggcggc
     1801 cgtggaggtg atacgaaata atgaaccgtt gtctgctgct taacctgtct caccgttctg
     1861 gtgaagattc gttccccgca ctctgcatct ctgctttgca tacctgccga tgttataccc
     1921 atctcggcgc ttctcaggat tcaagagctg gttacagtta ctgaggactg aacaagggcg
     1981 ctcttgtaaa aacaagagtt ttctcgtggt ttcgccgaac tgtcacactt acgttcggtt
     2041 atgtgcttaa taatgttatg aaaaagaaac cggttgcgca gttggagcgt cagcattcac
     2101 tgctggaaaa tccatgtgct tatgggttgt tatcgcagtt ccaggctgcg atagtcgtta
     2161 actgttttac acttaataaa ataatttgag gttcgctatg tctgacgaca tgtctatggg
     2221 tttgccttcg tcagcgggcg aacacggtgt actacgctcc atgcaggagg ttgcaatgag
     2281 ctcccaggaa gccagcaaga tgctgcgtac ttacaatatt gcctggtggg gcaataacta
     2341 ctatgacgtt aacgagctgg gccacatcag cgtgtgcccg gacccggacg tcccggaagc
     2401 tcgcgtcgat ctcgcgcagt tagtgaaaac tcgtgaagca cagggtcagc gtctgcctgc
     2461 actgttctgt ttcccacaga tcctgcagca ccgtttgcgt tccattaacg ccgcgttcaa
     2521 acgtgcgcgg gaatcctacg gctataacgg cgattacttc cttgtttatc cgatcaaagt
     2581 taaccagcac cgtcgcgtga ttgagtccct gattcattcg ggcgaaccgc tgggtctgga
     2641 agccggttcc aaagccgagt tgatggcagt gctggcacat gctggcatga cccgtagcgt
     2701 catcgtctgc aatggttata aagaccgcga atatatccgc ctggcattaa ttggcgagaa
     2761 gatggggcac aaggtctatc tggtcattga gaagatgtca gaaatcgcca ttgtgctgga
     2821 tgaagcagaa cgtctgaatg tcgttcctcg tctgggcgtg cgtgcacgtc tggcttcgca
     2881 gggctccggt aaatggcagt cctccggcgg ggaaaaatcg aagttcggcc tggcggcgac
     2941 tcaggtactg caactggttg aaaccctacg tgaagccggg cgtctcgaca gcctgcaact
     3001 actgcacttc cacctcggtt cgcagatggc gaatattcgc gatatcgcga caggcgttcg
     3061 tgaatccgcg cgtttctatg ttgagctgca caagctgggc gtcaatattc agtgcttcga
     3121 cgtcggcggc ggtctgggcg tggattatga aggtactcgt tcgcagtccg actgttcggt
     3181 gaactacggc ctcaatgaat atgccaacaa catcatctgg gcgattggtg atgcgtgtga
     3241 agaaaacggt ctgccgcatc cgacggtaat caccgaatcg ggtcgtgcag tgactgcgca
     3301 tcacaccgtg ctggtgtcta atatcatcgg cgtggaacgt aacgaataca cggtgccgac
     3361 cgcgcctgca gaagatgcgc cgcgcgcgct gcaaagcatg tgggaaacct ggcaggagat
     3421 gcacgaaccg ggaactcgcc gttctctgcg tgaatggtta cacgacagtc agatggatct
     3481 gcacgacatt catatcggct actcttccgg cacctttagc ctgcaagaac gtgcatgggc
     3541 tgagcagctt tatttgagca tgtgccatga agtgcaaaag cagctggatc cgcaaaaccg
     3601 tgctcatcgt ccgattatcg acgagctgca ggaacgtatg gcggacaaaa tgtacgtcaa
     3661 cttctcgctg ttccagtcga tgccggatgc gtgggggatc gaccagttgt tcccggttct
     3721 gccgctggaa gggctggatc aagtgccgga acgccgcgct gtgctgctgg atattacctg
     3781 tgactctgac ggtgctatcg accactatat cgatggtgat ggtattgcca cgacaatgcc
     3841 tatgccggag tacgatccag agaatccgcc gatgctcggt ttctttatgg tcggcgcata
     3901 tcaggagatc ctcggcaaca tgcacaacct gttcggtgat accgaagcgg ttgacgtgtt
     3961 cgtcttccct gacggtagtg tagaagtgga actgtctgac gaaggcgata ccgtggcgga
     4021 catgctgcaa tatgtacagc tcgatccgaa aacgctgtta actcagttcc gtgatcaagt
     4081 gaagaaaacc gatcttgatg ctgaactgca acaacagttc cttgaagagt tcgaggcagg
     4141 tttgtacggt tatacctatc ttgaagatga ataattaaga ttattctgct ggcgggtcga
     4201 tttatttacg taaatcggtc cgctttcttt ttatatattc ttaaaaataa tttagctgct
     4261 tttcttatta aataatctcc ctacaatcgc tgcattaatt aaatgcagag ggaacggtta
     4321 tgaaaaaatg gaaagtacgc agcgcgttgg tggcattaat cgtgctgctg gccggttgtt
     4381 ccagtaatgc gcagtataat agcagtgcat cgggcaatgt cggcaccgcc tggggtgggg
     4441 atgtgcactc aacggttcag ggcgtatctg ctgagcgagc ctggcgcgat ccggcggaga
     4501 tgatcgttat tagttattcg actaatgttc cttccggcta cgatcgcgtt tatagcattc
     4561 gtattaacga actggaatat gctattcgcg atggtaattt taattctcta cctattacgc
     4621 gtgtttatga ttcatctaat aatgaaccac gctatattgt ccatgccaga gtcggaatga
     4681 attatcaatt atatgtccgt aattacagtc gtaacaccaa ctacgagatc gttgctacgg
     4741 ttgatggcct ggatgtgctg aacggcaagc aaggttcgct gaataacaac ggctatattg
     4801 tgaatgccgg agattcgtta gcgattaaag gattccgcaa agataaacat accgaagcgg
     4861 cttttcagtt cgccaacgtt gctgattcct acgcagccaa ttcggcgcaa ggcgatgtgc
     4921 gtaataccgg tgtaatcggc tttgccgcct ttgaattaca aggccaggcg caaaacgcgc
     4981 tgccaccgtg ctctggacaa gcctttccgg cagataacaa tggctatgcg ccgccaccgt
     5041 gtcggaaata gcaacctcgc ttgatgcctc gcccatttaa ggcgataatt agcacaaact
     5101 tgtgtttcgc aaatccaacc cttcctcgtc gggcctaacg acgcggaagg gtttttttat
     5161 atcgactttg taataggagt ccatccatga gcaccttagg tcatcaatac gataactcac
     5221 tggtttccaa cgcctttggt tttttgcgcc tgccgatgaa cttccagccg tatgacagcg
     5281 atgctgactg ggtgattact ggtgtgccgt tcgatatggc cacttctggt cgtgcggggg
     5341 gacgccacgg tccggcagct atccgtcagg tttcgacgaa tctggcctgg gaacacaacc
     5401 gcttcccgtg gaatttcgac atgcgtgagc gtctgaacgt cgtggactgt ggtgatctgg
     5461 tatatgcttt cggcgatgcc cgtgagatga gcgagaagct gcaggcgcac gccgagaagc
     5521 tgctggctgc cggtaagcgt atgctctcct tcggtggtga ccactttgtt acgctgccgc
     5581 tgctgcgtgc tcatgcgaag catttcggca aaatggcgct ggtacacttt gacgcccaca
     5641 ccgataccta cgcgaacggt tgtgaatttg accacggcac catgttctat accgcgccga
     5701 aagaaggtct gatcgacccg aatcattccg tgcagattgg tattcgtacc gaatttgata
     5761 aagacaacgg ctttaccgtg ctggacgcct gccaggtgaa cgatcgcagc gtggatgacg
     5821 ttatcgccca ggtgaaacaa attgtgggtg atatgccggt ttacctgacc tttgatatcg
     5881 actgcctgga tcctgcattt gcgccgggaa ccggtacgcc ggtaattggc ggtctgacct
     5941 ccgatcgcgc tattaaactg gtacgcggcc tgaaagatct caacatcgtt gggatggacg
     6001 tagtggaagt ggctccggca tacgatcagt cggaaatcac cgctctggca gcggcaacgc
     6061 tggcgctgga aatgctgtat attcaggcgg cgaaaaaggg cgagtaagca ccagatgcga
     6121 tgcgcacggg taaaacgtgc cattaatgtc ggatgcggcg tgaacgcctt atccgaccta
     6181 cgttcggcac ccgtaggccg gataagatgc gccagcatcg catccggcaa tgcgcacaag
     6241 gtaacaaatg tgccattcat gtcagatgcg gcgtgaacgc cttatctgac ctacgttcga
     6301 caccaccagg ctttacttaa tcccatccgc gctcatgcga tcgcgaatat gctgggcgcg
     6361 ttcggcggat gcaggatggt cgtcaaacat tgagctttgg cgaccttctt ccagttttgc
     6421 cagtttttca aagctggtgg caagacctgc cgggctgatg ccgcgttggc gcagaagatc
     6481 gtaagaataa tcatcggctt ctgcttcctg gcgctgggag aattgcgaat taactaattt
     6541 ctcgcccaga tcaccgagtt gtgattgaga taaactgccg acaatcccgc ccgccgaggc
     6601 cgcagctact cgtacggcat ttgtaccaag tgccacctgc attccttttt tcacatggcc
     6661 taatgccacg tgccccattt cgtgaccgat caccgcttcg acttcgttat ccgtcatcat
     6721 atccatcagc ccgctataga cacggataca gccgttagcc attgcaaagg cgttcacatc
     6781 cttcgccata tacactttgt aatttaccgg ctgaccgttg atattgttgc ctagcgcatt
     6841 ggcaatagtt gtcagacgtt tagcgtattc gctattggct ggcgcaatcg tcgccttgct
     6901 gtccatctcc tgacatgcct gatcgctcag ggttttcacc tgcgcatcac tcaaactgta
     6961 agcctgaaaa gcttccgctc ctgatgagag cagtccgttg gagtccatat tctggcaacc
     7021 ggtcagtacc gttgccacgc tcattgctac caataaggcg cgaattttca tgttttttct
     7081 tccgcacaag gtcaggatta ttctaaaaat gcgccgtttg caggtgaacc gacgctcagt
     7141 ctcagtataa ggaaaagcgc agcggacggg cgagtagatt gcgcaacatg cgagcatgat
     7201 ccagagattt ctgaagcggc aaaaggatgt tccatgtaca tgacgcgcgg cttgcggtaa
     7261 attgttggca aattttccgg cgtagcccaa aacgcgctgt cgtcaagtcg ttaagggcgt
     7321 gcccttcatc atccgatctg gagtcaaaat gtcctcacgt aaagagcttg ccaatgctat
     7381 tcgtgcgctg agcatggacg cagtacagaa agccaaatcc ggtcacccgg gtgcccctat
     7441 gggtatggct gacattgccg aagtcctgtg gcgtgatttc ctgaaacata acccgcagaa
     7501 tccgtcctgg gctgaccgtg accgcttcgt gctgtccaac ggccacggct ccatgctgat
     7561 ctacagcctg ctgcacctca ccggttacga tctgccgatg gaagaactga aaaacttccg
     7621 tcagctgcac tctaaaactc cgggccaccc ggaagtgggt tacaccgctg gtgtggaaac
     7681 caccaccggt ccgctggggc agggtattgc caacgcagta ggtatggcga ttgcagaaaa
     7741 aacgctggcg gcgcagttta accgtccggg ccacgacatt gtcgaccact acacctacgc
     7801 cttcatgggc gacggctgca tgatggaagg catctcccac gaagtttgct ctctggcggg
     7861 tacgctgaag ctgggtaaac tgattgcgtt ctacgatgac aacggtattt ctatcgatgg
     7921 tcacgttgaa ggctggttca ccgacgacac cgcaatgcgt ttcgaagctt acggctggca
     7981 cgttattcgc gacatcgacg gtcatgacgc agcatccatc aaacgcgcag tagaagaagc
     8041 gcgcgcagtg actgacaaac cgtccctgct gatgtgcaaa accatcatcg gtttcggttc
     8101 cccgaacaaa gccggtactc acgactccca cggtgcgccg ctgggtgatg cagaaatcgc
     8161 tctgacccgc gaacagctgg gctggaaata cgcaccgttc gaaatcccgt ctgaaatcta
     8221 tgcgcagtgg gatgcgaaag aagcaggcca ggcgaaagaa tctgcatgga atgagaagtt
     8281 tgcggcttac gcgaaagctt atccgcagga agcggctgaa tttacccgcc gtatgaaagg
     8341 cgaaatgccg tctgacttcg acgccaaagc gaaagagttt atcgctaaac tgcaggctaa
     8401 tccggcgaaa atcgccagcc gtaaagcgtc tcagaatgct atcgaagcgt tcggcccgct
     8461 gttgccggaa ttcctcggcg gctccgctga cctggcaccg tctaacctga ccctgtggtc
     8521 tggttctaaa gcaatcaacg aagatgctgc gggtaactac atccactacg gtgttcgcga
     8581 gttcggtatg accgcgattg ctaacggtat ctccctgcac ggtggcttcc tgccgtacac
     8641 ctccaccttc ctgatgtttg ttgaatacgc acgtaacgcc gtacgtatgg ctgcgctgat
     8701 gaaacagcgt caggttatgg tttacaccca cgactccatc ggtctgggcg aagatggccc
     8761 gactcaccag ccggttgagc aggttgcttc tctgcgcgtg accccgaaca tgtctacatg
     8821 gcgtccgtgt gaccaggttg aatccgcggt cgcgtggaaa tacggtgttg agcgtcagga
     8881 cggtccgacc gcgcttatcc tctcccgtca gaacctggcg cagcaggaac gtactgaaga
     8941 gcaactggca aacatcgcgc gcggtggtta tgtgctgaaa gactgtgctg gtcagccgga
     9001 actgatcttc atcgcgaccg gttcggaagt tgaactggct gttgccgcct acgaaaaact
     9061 gactgccgaa ggcgtgaagg cgcgcgtggt ttccatgccg tctaccgatg cattcgacaa
     9121 gcaggatgcg gcttaccgtg aatccgtact gccgaaagcg gttactgcac gcgttgcggt
     9181 agaagcgggt attgctgact actggtacaa gtatgttggc ctgaatggcg ctatcatcgg
     9241 tatgaccacc ttcggtgagt cagctccggc agagttgctg ttcgaagagt tcggcttcac
     9301 cgtcgacaac gtcgttgcga aagcaaaaga actgctgtaa ctagcatttc gggtaaaaag
     9361 gtcgcttcgg cgaccttttt tattaccttg atatgtccgt ttgcggacaa gcaatagata
     9421 aagcgtgttg tagatcgcaa atatttatat gcaataaata tcaattatgt aatatgcatc
     9481 acgatatgcg tgttgacatt tgttgttata tctataactc aatgttatat aagaaattaa
     9541 taattcactg ttttcaaaac accggtttcc ctgctcaatt gctttcatta aaccgcacga
     9601 gtattttatt tttaccgccg gacggtaccc taaaaataaa ataaggataa tttatgcggc
     9661 ttagtgacta ttttccagaa tcatcaatct cagttataca ttcagcaaaa gattggcagg
     9721 aagctatcga tttctcgatg gtatcattgc tggataaaaa ctatatcagc gagaattaca
     9781 ttcaggcaat taaagattcc accattaaca atggccctta ttatattctc gcaccaggcg
     9841 tggcaatgcc tcatgcgcga ccggaatgtg gggcgcttaa aaccgggatg tctttgacat
     9901 tacttgaaca aggtgtttat tttccgggga atgacgaacc aattaaatta ctcatcggac
     9961 tctctgctgc cgatgccgat tcgcacattg gcgctattca ggcgttaagt gaattactgt
    10021 gcgaagaaga aatactcgaa caactcttaa cagcatcatc agaaaaacaa ttagcggaca
    10081 ttatcagccg cggataatta ctctctcctc tacaggataa tattatggaa aacaagtctg
    10141 ctcgtgcaaa ggtccaggct tttgggggct ttttgactgc aatggtcatc cccaatattg
    10201 gtgcttttat tgcctggggt tttattactg cgttatttat tcccaccggt tggctgccta
    10261 acgaacattt cgccaaaatt gtcggcccga tgattaccta tttattgccc gtgatgattg
    10321 gttctaccgg tggtcatctg gtcggcggta aacgcggcgc ggtcatgggc ggaataggta
    10381 ctattggtgt gatcgtcggc gcagagatcc cgatgttcct tggctcaatg attatggggc
    10441 cgctcggtgg tctggtcata aaatatgttg ataaggcact ggaaaaacgc atacctgccg
    10501 gttttgagat ggttatcaat aacttctcat taggtatcgc ggggatgctc ctttgcctgc
    10561 tgggttttga agtgatcggc ccggcggtgt taattgccaa tactttcgtc aaagagtgta
    10621 ttgaggcgct ggtacatgcg ggttatctgc ctctgctgtc agtcatcaat gaaccggcga
    10681 aagtgctttt ccttaataat gcgatcgatc agggcgtcta ttatccgctg ggaatgcaac
    10741 aggcttcggt taacggtaaa tccatcttct ttatggtggc ctctaaccca ggtccgggcc
    10801 tggggctgct gctggcgttt accttgtttg gtaaagggat gagtaaacgt tctgcgcccg
    10861 gggcgatgat tattcacttc ctcggtggga tccacgaact gtatttccca tatgtgctga
    10921 tgaagccgct gactattatt gccatgattg cgggcggtat gtctggcacc tggatgttta
    10981 acttactgga cggtggtctg gtggctggcc caagtccggg gtctatcttt gcttacctgg
    11041 cgctgacgcc gaaaggttcg ttcctggcga caattgccgg tgttacggta ggtaccctgg
    11101 tgtcctttgc tatcacttcg ctgatactga agatggaaaa aacggtggaa acggagagcg
    11161 aagatgagtt tgctcagtca gccaatgcgg ttaaggcgat gaaacaagag ggtgcattct
    11221 cgttaagcag ggttaagcgt atcgcctttg tttgcgatgc ggggatgggc tccagtgcga
    11281 tgggcgcgac caccttccgt aaacgcctgg aaaaagcggg gctggcaatt gaagtaaaac
    11341 attacgccat agaaaacgtg cctgcggatg cggatatcgt cgttactcat gccagtctgg
    11401 aagggcgcgt gaaacgtgtg acggataaac cactgatatt gattaataac tatattggcg
    11461 atccaaaact cgacacttta tttaatcaat taaccgccga acataaacac tgattacaga
    11521 ggtaaaaatg aaaaccaaag ttgctgctat ttatggcaag cgggatgtcc gtctgcgcga
    11581 atttgaactg ccagaaatta ccgataatga attactggtg agtgtgattt ctgacagcgt
    11641 ctgtttatcc acctggaaag cggcgttact cggtagtgaa cataaacgcg tacccgacga
    11701 tttagaaaat catccggtca ttaccgggca tgaatgtgcc ggggttattg tcgaagtggg
    11761 taaaaatctc actggcaaat ataaaaaagg ccagcgtttt gtattgcaac cggcgatggg
    11821 gttaccaagc ggatattcag cgggctacag ctacgaatat tttggcggca atgccactta
    11881 tatgattatt cccgaaatcg ccattaattt gggctgcgta ttaccgtatc acggctctta
    11941 ttttgctgcg gcgtcgctgg cagagcctat gtgctgcatt attggcgctt atcatgccaa
    12001 ttatcacacc acgcaatatg tttatgagca tcgcatgggc gtcaaacctg gcggcaatat
    12061 tgcactgctg gcgtgtgcag gtccgatggg cattggcgct atcgattacg ccattaacgg
    12121 cggcatacaa ccgtcgcggg tggtggtggt cgatatcgac gacaaacgtc tggcgcaggt
    12181 acagaagctg ctgccggtgg aactggcggc cagtaaaggc attgagctgg tgtatgtgaa
    12241 taccaaaggg atgagcgatc ctgtccagat gctgcgggcg ctgacaggag atgccgggtt
    12301 cgatgacatt tttgtttatg cggcggtgtc tgctgtcgtt gagatggctg atgaattact
    12361 ggcggaagat ggctgtctga acttctttgc cgggccgacg gataaaaact tcaaagtgcc
    12421 gtttaatttc tacaacgttc attacaacag cacgcacgtc gtcggtacat ccggcggttc
    12481 aacggacgac atgaaagagg cgattgccct tagcgccaca gggcagttac agccctcgtt
    12541 tatggtgacc catatcggtg gcctggatgc ggtgccagaa accgtgctca atctgccgga
    12601 tatccctggc ggtaaaaaac tcatttataa cggcgtgacc atgccgctca ctgccattgc
    12661 cgattttgcc gaaaaaggca aaaccgatcc gctgtttaaa gagttggcgc ggctggttga
    12721 ggaaacgcac ggcatctgga atgaacaggc cgagaaatat ctgctggcac aatttggcgt
    12781 tgatatcggg gaggccgcgc aatgatgtcc ctggcgtggc cattatttcg cgttacggaa
    12841 caggcagcgt tggctgcctg gccgcaaacc ggatgtggcg acaaaaataa aattgatggc
    12901 ctggcggtca ccgcgatgcg ccaggcatta aacgacgtcg cttttcgtgg acgagtggtt
    12961 atcggcgaag gagaaattga ccatgcgccg atgctgtgga ttggcgaaga ggtgggcaaa
    13021 ggcgatgggc cagaagtcga tatcgcggtt gatcccattg aaggcacgcg gatggtggcg
    13081 atggggcaga gcaatgcgct ggcggtcatg gctttcgccc ctcgagatag cctgttacat
    13141 gcgcccgata tgtatatgaa aaagctggtt gttaatcgac tggctgccgg ggcgattgat
    13201 ctgtctttgc ctctggcgga caacttgcgt gacgtggcgc gggcgttagg taaaccgctg
    13261 gacaagctgc gcatggttac gctggataaa ccgcgtctga gtgccgcgat tgaagaagca
    13321 acacagctgg gcgtgaaggt ttttgccctg ccggatggcg atgtcgccgc cagcgtgctg
    13381 acctgctggc aggataatcc ttacgatgtg atgtacacca tcggcggtgc gccggaaggc
    13441 gtgatttctg cctgtgccgt taaagcatta ggcgggaata tgcaggccga gctgattgac
    13501 ttttgtcagg ccaaagggga ttacacggaa aatcggcaga ttgcagagca ggagcgcaag
    13561 cgttgcaagg caatgggtgt tgacgttaac cgtgtgtact cgctcgatgt actggtaagg
    13621 gggaacgata tcctctttag cgctacgggc gtcacgggcg gcgagttggt gaacggtatc
    13681 caacagacgg cgaatggagt gcggacgcag acattactga tcggcggcgc ggaccaaacg
    13741 tgtaatataa tagactccct gtattgatgg tgatctatcg gaagaccaat ggcgacgctg
    13801 tcagaagatg atgtgcttga gcaactggat gcacaggaca atttattttc atttatgaaa
    13861 actgcgcatt ctattttgct ccaggggata cgccagtttc tgccgtcgct gtttgtcgat
    13921 aacgatgaag agagcgtcga atatgcagtg aagccgttac tcgcccaaag cggcccgctt
    13981 gacgatattg atgttgcact gcgtttgatt tatgcgctgg ggaaaatgga taaatggctg
    14041 tacgccgata tcacgcattt ttcccagtac tggcattacc tgaaagaaca ggatgaaacg
    14101 ctcggatttg ccgatgacat gacttgggat tttatcagca acgtcaatag cataacctgc
    14161 aatgcgacgc tctatgacgc gttgaaagcg atgaagttcg ccgatttcgc cgtctggtca
    14221 gaggcgcgtt ttagcggaat ggtcaaaacg gcgctgacgc tggcagtaac gaccacctta
    14281 aaggaattaa cgccgtgaaa attgaattaa cggtgaatgg gctgaacgtg caagcgcagt
    14341 accatgatga agagatagaa cgcgtgcata aaccgttgct gcgtatgctg gcggccttac
    14401 agacggtaaa tccgcagcgc cgaaccgtgg tttttctttg cgctccgccg gggacgggta
    14461 aatctaccct aaccaccttc tgggcatatc tcgcgcaaca agacccggag ctgcccgcta
    14521 ttcagacgct cccgatggat ggttttcacc attacaatag ctggctggat gcgcatcaat
    14581 tgcgcccctt caaaggcgca ccagagacat ttgacgttgc gaaactggcg gaaaatctgc
    14641 gccaggtcgt ggaaggggat tgtacgtggc cgcagtacga tcgacaaaag catgatcctg
    14701 ttgaagatgc gttgcacgtt accgcaccaa taatcatcgt cgaaggaaac tggctattgc
    14761 tggatgacga taactggcgc cagctagcgc aattttgtga tttctcgatc tttatcaatg
    14821 cgccagctac tgcgttgcgg gaacgactgg tggggcgcaa gctggcggga gggttatcgc
    14881 tggcagacgc agaagctttt tatgaccgta ccgacgggcc gaatgttcgc cgggtgctgg
    14941 aagagagtct gcctgcaaat ttgaccttaa tgatgaccgc tacgggtgaa tatcgtctgg
    15001 tggattaatg ttcctgatta gaccatcttc cagtcgcacc acacgagaga aatgacgccg
    15061 ggtaaatgcg gcgtcatgac tgattgctac gacgctcgtg cctcgctggc ggcacttctc
    15121 cagccagctt tcaaacacgc tcaaccaatt ttcgtcaaaa tcccgactcg gctcgtccag
    15181 cagcaatagt ggcggagaga gtgcttccag gcaggcgacg gcgaccatcc gtcgctgcgc
    15241 actatgcaga tccaacgggt gcgcgctggc agtgtctgct aactgacaac attgcagggc
    15301 agcatgggta cgttgagtga tttcatccgc cgggcatttt tgcagtttca aaccaaatgc
    15361 gatctcgtca gcgacggtgc tgtgaaaaag ttggttttcc gcttcctgaa acagtacgcc
    15421 aatctttgcc gcgcgctggc ggtttttcag gtttttcatc gcctgttgtt gcagcataac
    15481 cgtaccggct gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg atttccctgc
    15541 gccattgtcg ccggtcaacg ccaaccattc accttgtttg agttgcagag aaatgtcgca
    15601 aagacaatcc gttgccgcgc caggccagcg ataagatatc tgatttaacg ttaacatagt
    15661 ggctggagcg ctccgttttg caattgccac gcttgctggc agtagttcag gaaaggtgtg
    15721 cgatgacgtt caaaaagaat aatcagcgaa ccacgttcaa acgcccagtg ttgtaagcgc
    15781 tgcaacagca tttcgctggc ctgcggcgtc aggcggctaa aggcttcatc aagaattaat
    15841 agctttggct gcatcgcgat ggcgcaggcg atgaccacgc gctgtgtctc tccaccggag
    15901 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca
    15961 atccgcgcca taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
    16021 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
    16081 atggtggcgg attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc
    16141 gtgcctgtta tctctcctgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc
    16201 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
    16261 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac
    16321 gtaaccatat ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta
    16381 cgcgttgtag ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa
    16441 atgcccgcat atccagcgcc gcaccacgga cagcaagatc gtttagcgca ttgtgtgtta
    16501 gcggaatagt taacgcgggc atcgcacgta aacgctgata ccagccctca tccagcggca
    16561 cgccgcgggc acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga
    16621 ccaataacgg cccggcaaac agataagcga cgcccggcgg caggcgagaa gcaaacagag
    16681 cgcggataaa acattgcacg gggacgtact gcatccacaa ctgtgaggtt gaaacaatcg
    16741 ccagaatccg caaccagagc gtaatagcat gcgtccaacg ctccggcgaa cgcggcgtgc
    16801 cgcttagcca ttctgttagc cagccaccgt gtaccagcca cagcccggct cccagcgaga
    16861 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaaa
    16921 aaaaggtcgc cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc
    16981 aggccgccaa tgcccatagt gttaatgagg taaatggatg catttagcgc acggcagcca
    17041 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca
    17101 ccgctgtaag agttttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
    17161 ccaggttttg ccccatcgag tgcatccagg caacgaacaa atcggctccg ctaccggtga
    17221 caccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
    17281 tcacgccgct gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac
    17341 cggcgaccag gccgatgacc atcgcgacgg gggcaaaagc ggcagcgatc gggtcggtca
    17401 gtaatcccca gagtaaattg gtcagtaagc cggtcagcat cccgatcacc ggaccaagca
    17461 gtaccgcgct aataagtgtg ccgatggagt cgagaaatat aggtaatttg accatgctgg
    17521 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
    17581 tggaaaagtg gctacgcgcc ataagaaaac ctctctgaaa gtagttattt aacgggggaa
    17641 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
    17701 aggctaccga tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
    17761 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
    17821 aacgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
    17881 ccggcatagc gcaaggcgat gtcagtcagg ctaccggtac agcctgcaat ccaggcggca
    17941 tgaagttgct gctgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg
    18001 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgggt agggcaatgt catcattgga
    18061 gtactccgaa actcggacgt attacggata atatcgacgc tgcatcagtc atattctgat
    18121 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
    18181 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgt
    18241 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
    18301 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
    18361 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
    18421 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
    18481 tcgtgctttg tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact
    18541 ggcggatgct gcgggcatgg cgcatttgtt gaaatacgac accagccatg gccgttttgc
    18601 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
    18661 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
    18721 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg cacattgccg ccggggctaa
    18781 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
    18841 caatcaggat caacttcgtg cggagcaccg catcgtttct aacgcttcct gtaccacgaa
    18901 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
    18961 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
    19021 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgccgg
    19081 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
    19141 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
    19201 tgaagtcaac ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
    19261 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
    19321 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
    19381 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
    19441 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
    19501 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
    19561 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
    19621 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
    19681 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
    19741 aagacaaact gtctaacccg gttcgtctgg ttaaagatta cctcgacggc gttgacgttg
    19801 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
    19861 acgaaatcct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
    19921 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
    19981 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaggcactg aaagaacctg
    20041 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
    20101 actccctgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
    20161 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
    20221 caaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
    20281 agttctctga aaccgctccg gctaccctga aatctgttaa cgacgtgaaa gctgacgagc
    20341 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
    20401 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
    20461 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
    20521 acactctggc agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg
    20581 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
    20641 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcccttg tttcagcgcg
    20701 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
    20761 gagtgaatcc ccaggagctt acataagtaa gtgactgggg tgagcgaacg cagacgcagc
    20821 acatgcaact tgaagtatga cgagtataag gcccgacgat acaggacaag agacatgtct
    20881 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
    20941 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
    21001 atcaacgccg tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
    21061 aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct
    21121 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
    21181 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
    21241 ctgctggacg cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac
    21301 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
    21361 gagcgcatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
    21421 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
    21481 gttgattacg catacaccga actgagcaaa atcagcccgc gtttcaccat cgcagcgtcc
    21541 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
    21601 cgtgattctc aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc
    21661 gtattccacg gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc
    21721 gtagtgaaaa tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
    21781 tactacaaag cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat
    21841 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
    21901 atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagtcatt
    21961 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaaattgc
    22021 ctgatgcgct atgcttatca ggcctacacg tatcctgcaa tatattgaat ttgcaggttt
    22081 ttgtaggccg gataaggcgt tcacgccgca tccggcatga caaagcgcac attgatatct
    22141 gtctgagccc cgctctgcgg ggattttttt gccaaaagca aggtgattca gatgagaaat
    22201 ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc ttgtcagact gcccgtcata
    22261 aggcggcgga gtgtatttct ccattttgag tcagttgaaa aggaatattg aatggaagat
    22321 ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc tggtagctaa ccaggcgctg
    22381 ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga tcatcatcgt tggtttgatt
    22441 atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga tctcccgtaa aatcgatgcc
    22501 actgttgctg attttctttc tgcattagtc cgttacggta ttatcgcctt tacgctaatc
    22561 gcagccctgg gacgcgtggg tgtacaaaca gcgtcagtca ttgctgtact cggtgccgca
    22621 ggcttagctg ttggtctggc tttgcagggg tcactttcta acctggccgc tggcgtgtta
    22681 cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg acctcggcgg cgtagccggt
    22741 actgtgctga gtgtgcagat tttctccacc accatgcgta ctgcagacgg taaaattatc
    22801 gttattccga acggtaaaat tattgccgga aatattatta acttctcccg cgagccagtt
    22861 cgccgtaacg aatttattat tggcgtggcg tatgattccg atatcgatca ggttaagcag
    22921 atcctgacca atattatcca gtctgaagat cgcattttga aagatcgcga aatgactgtg
    22981 cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg tccgcgtctg gagcaacagc
    23041 ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta ttaaacgtga atttgatgcc
    23101 gccggtatca gcttcccgta cccgcaaatg gatgtgaact ttaagcgggt gaaagaagac
    23161 aaagctgcgt aatcaacgct gaggccagat aatactctct ggcctctctc ttattagtta
    23221 ttctgattgc caattaatat tatcaatttc cgctaataac aatcccgcga tatagtctct
    23281 gcatcagata cttaattcgg aatatccaac gtgttttctt attactttca aggtcttgct
    23341 cttggggcag ctatgattct gccgctcggc ccacaaaatg cttttgtgat gaatcagggc
    23401 attcgtcgtc agtaccacat tatgattgcc ttactttgcg ctatcagcga tttggtcctg
    23461 atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc agtcgccgtg gttgctggcg
    23521 ctggtcacct ggggcggcgt agtcttcttg ctgtggtatg gttttggcgc ttttaaaaca
    23581 gcgatgagta gtaatattga gttagctagt gccgaagtcc tgaagcaagg cagatggaaa
    23641 attatcgcca ccatgttggc agtgacctgg ctgaatccgc atgtttacct agatactttt
    23701 gttgtactgg gcagccttgg cgggcagctt gacgtcgaac caaaacgttg gtttgcgctc
    23761 gggacaatta gcgcctcttt cctgtggttc tttggtctgg ctattctcgc agcctggctg
    23821 gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca atctggtagt gggatgtgtc
    23881 atgtggttta tagccttgca gctggcgaga gacggtattg ctcatgcaca agccttgttc
    23941 agttaggcgc tatctgatgg aaaaataaaa cagaggcgct aagcttgcct ccagagatcc
    24001 tgaattttgg gcaagtcaat taacacggag agactaacgt gaagttcaaa gttatcgccc
    24061 tggcggcatt aatgggtatt agcgggatgg cagcgcaggc taacgaattg ccggatggac
    24121 cgcatattgt cacctccggt acggcaagtg tggatgcggt gccagacatt gccactcttg
    24181 cgattgaagt taacgtggcc gcgaaggatg ccgctacagc caagaaacag gcagatgagc
    24241 gcgtcgcaca atacatttcc ttccttgaac tcaatcagat cgcgaaaaaa gatatcagct
    24301 cagcgaattt acgcacccag ccagattatg attatcagga tggtaaaagt atcctgaaag
    24361 gctaccgcgc tgtgagaacg gtggaagtca cgctccgtca gttagacaaa ctgaattcct
    24421 tgctggatgg cgcgctgaag gcgggtctta acgaaattcg ttcagtgtcg ctgggcgtgg
    24481 cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc gattgataac gcgattcatc
    24541 aggcgcagga actggcgaac ggctttcatc gtaaactggg gccggtatat agcgtgcgct
    24601 accatgtttc caactatcag cccagcccaa tggtgcggat gatgaaagcc gatgccgcgc
    24661 cggtgtctgc ccaggaaact tacgagcagg ccgctattca gtttgatgat caggtcgatg
    24721 tggtcttcca gttagaacct gtggatcaac aacccgctaa aacacctgca gcacaataat
    24781 acttgtagcc cgacgtattc gtcgggctaa tgcctttacc cgatattgcc tcctttttct
    24841 gtaattttct gtaaaaccat gacgtaatca cctatctttg ctcgcggttt catcaaggat
    24901 aggctgactg aaacggttaa gcggacaagg atgcggggag gggggatgga catttttatt
    24961 tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca ataatattgc ccgggcggca
    25021 gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca ttgccgcgct ggaagaacaa
    25081 attggctata cgctatttac ccgcaaagat aataatatca gcctcaataa agccggtcag
    25141 gaactgtatc aaaaactgtt cccggtttat caacgacttt ctgcgattga taatgaaatc
    25201 cataactcag ggcgtcgttc acgagaaatt gtgattggca tagataatac ttatccgaca
    25261 attattttcg atcagttgat tagtctcgga gataagtatg aaggcgtgac cgcccagcca
    25321 gttgagttca gcgaaaacgg cgttatcgat aatctttttg accgtcagtt agattttatt
    25381 atctccccac agcatgtatc tgcacgagtt caggaactgg aaaacctgac tatcagtgaa
    25441 ttaccaccgc tgcgattgaa ctttctcgtt tctcgccgtt atgaagagcg tcaagagcaa
    25501 gagctgcttc aggagctgcc ctagctacaa atgcgctttc aaaaccgggc taacttcgaa
    25561 gcaatgattg atgcgaatat gcgaccttgt gggatcaatc ccaccattat ttatcgtccc
    25621 tacagtttta tggccaaaat cagcgccgtg gagcgcggac attttctgac ggtgatcccg
    25681 cattttgcct ggcgactggt gaatccggca acgctgaaat attttgatgc gccgcacagg
    25741 ccgatgtata tgcaggaata tctttattca atcagaaatc atcgttatac cgccacgatg
    25801 cttcagcata ttgctgaaga tcgtgacggg acaaaccatt aacccagcat cgagccggtt
    25861 gcaattaaat tacggtgtaa gtcgaagacg tggctaagat cgtggtgaat atgtccgccg
    25921 ggcgcatttt ccagatagcg atgcagataa tcccgataca taggatgtgc acaattatca
    25981 atgatagtgc gggcgcgttg aagcggggaa agaccgcgca gatcggcgat cccttgttcg
    26041 gtgatgatca ctttgacgct gtgttcgttg tgatcaacat ggctgcacat tggcacgacg
    26101 gttgagatct tgccttcttt agcaatcgac ggagccataa agatcgacag atacgcgttg
    26161 cgttcaaaat caccgctgcc gccgatgccg ttcatcagat cgaccccggc tacgtgcgtt
    26221 gagttggcat gcccgtaaat atcaaactcc aggccgacgt tcagagcgat gacgcccaga
    26281 cgacggatga tttccgggtt attggaaatc tcctgcggac gcaacacaat gcggctggca
    26341 aagtaatcca tattgtcgta aatcttgcgc agggaatcgg ccgagattgt caggctggag
    26401 gcgctggccc cgctgatttt gccggtttcc agtaaatgca ccaccgattc ctgtagcact
    26461 tccgaataca tcataaacgg aggaatttcc gggttttccc ccagacgcgc cattaccgca
    26521 ttattgatat tgcccacgcc actttgcagc ggcagaaatt ccggcggaat acgcccatgc
    26581 gccatttcct gcaataagaa cgtgaccacg ttatcggcaa tctgctggca catgggattt
    26641 tgcttatcca gcatattacc ggcgtcgggc aagttggttt ccacgacggc gacaatcttt
    26701 ttcggatcga tttgcacata gcgggtaccg acgcgatcca ttgcatggaa gatcgacacg
    26761 ctattgcgcc gcggtggcgc gccaggaatc acaatatccg ccagttctgc aacgcgcgga
    26821 tcgtgatagt gattgagttc aatgatcact ttcttcgctc gcagcagcca ggtcggcgca
    26881 ttaccgatcc cgctggttaa ccagactcga ccatctggtg ccagtgccga tgcttcaatg
    26941 acggcaacat caatgtcgcc gaagaaaccg taattgacca tttgcgccac ttcgctcaaa
    27001 tgcaggtcaa cgaaactcac cgcgccctga ttgatctttt tacgtaaacc ggacgatgtt
    27061 tgatatggcg cacgccagga aacagcatcg gcgtcagaaa gtacatcgtc agcggcggcg
    27121 ctgattgacg cccccgtcag caggcgaatt tgatacggct ttttggcctc atgctgttcg
    27181 ttagctctgc gggcaatcgc ggtgggtagg gctttcggcg aacccgctgg ggtaaagccg
    27241 ctaaatgcca ccatgtcgtt atgctggata atttctgccg cttcatcggc ggtcatcctt
    27301 gtccactgag tttccatttt tactccttcg ttcgcaggga ttaatgacca acgaaattag
    27361 gtttacgttt ttcgaggaaa gcgttcatcc cttcctggta atcttcgctg tcataaaccg
    27421 cgcggcgcat cccctgaata cgttcaaatt catcggagtt catggtgtgt gcttcgccca
    27481 gtacacgcag ctcttctttg ataacggcaa tggctaacgg cgctttctca gagatgtggt
    27541 gcgccatttg taaggtgaaa tcttccagtt cttccacttc cacaacatgg ttgaggatgc
    27601 cgacagccag cgcgcgctgg gcggtgatgg gcgaagcggt aaaaatcagc tctttgacaa
    27661 tgtggaagcc cgcgtcgcgg gtcaggttgt gaatgccgac caggttatac gggacgccga
    27721 ggtttacagg cgtcattgag aaggttgagg tactggcggc gataatcaga tcggaactca
    27781 tgatcatttc aaatgcgcca ccccaaacac taccttccac catcgaaata atcggtttcg
    27841 ggaatttttg gatcatgcgg gtgatttgac gcaatggatc atcataggag agcggatcgc
    27901 gaccgccaga cggcagttcg tgaatatcgt gacctgcgga gaagactttg gatccactcg
    27961 gcgcgcgcaa aatgatacag cgaatttccg gccggttgag atcgcttaac gcctgcataa
    28021 gatcatcaat aaagacttta cttaaggcat taagttttcg gccatagtta aactcaatga
    28081 ccgccacttt gttgatagtg acaacgttaa catactgata agacataaaa attcctttaa
    28141 tcaaaatatt gcgtctggat aaattcactg agctgccgca ggccggtgcg cggtgagagc
    28201 gtattgtttt tgaccgctaa aagcgtctgg cggtaatagc gatcgaaatc ttcattcgcg
    28261 aacaggtgat tcagtacttc ttcttcggtc tgcttacgca gccattccac cgattgttgt
    28321 tgccgcactt gttgtaaacg accactggca gttagcgcgg ttttgaagtc gatgatggcg
    28381 tgccagatct catcgattcc acgtttttcc agtgcgctac aagtcagaac ccgtggctgc
    28441 cattcgtcgt atttacgtcg cagaatatgc agggcactct cgtacatatg ccgggcaatg
    28501 gcgacattgg tatggttatc gccatcgtct ttgttgataa cgatcagatc agccacttcc
    28561 atcagccctt ttttaatgcc ctgcagatca tcgccgccac cggcaatttg caacgagata
    28621 aaacagtcca ccatgcgggc gacttctgtt tccgactgcc cgacgccaac cgtttcgaca
    28681 atcactacgt cataacccgc tgcttcgcat aacagcatta attcccgcgc tcgctgactg
    28741 gcaccgccca gatgaccgga ggatggtacc gggcgaataa acgccgcttc ggcacgcgcc
    28801 aggtcattca tgcgggtttt atccccgaga atgctaccgc cagtgaccgg gctgctggga
    28861 tcgaccgcaa taaccgcgac ctttaatccc tctcgaatca acaacatgcc aaaggcctca
    28921 agaaaggtac ttttccccgc gccgggggtg ccggtaacgc ccagtcgcag ggtgttaccg
    28981 cagtacggca taatggcatc aagcagctgc gtacttagtg cctgatgacg cgggtgacgg
    29041 ctttccacca gcgtcatggc ctgggcgagt gtggcatgct caccctgacg taagcggcga
    29101 atactttctg ccagcgtggc ttcattaatc atgatgctgg cttatcagat tcagtacgtc
    29161 gcgcacactg tcgagcatag gtgtacctgg accataaatc gccgccacgc cgcgctcttg
    29221 caggaaggcg taatcctgcg gtggaatgac gccaccggcg actacgcaga tatcttcgcg
    29281 tccccatttt ttcagcgctt cgaccagttc cgggatcagc gttttatgac cggcagccag
    29341 tgaggatgcg cccactacgt gaacgtcatt ttctactgcc aggcgggcga tctcttcagg
    29401 tgtagagaac atcgggctta aatctacgtc gaaaccgaga tcggaatagg cgctggcgat
    29461 cactttcgcg ccgcgatcgt gtccatccag gcccattttg gcgatcagaa tgcgcgggcg
    29521 acgaccattg tcggcaagga actgctccgt ttgcgcaaca atggcatcga actcggaggc
    29581 cgatttctca gattgatgat agctttgcgc aatcacgccg gtaacacact ggcttggcac
    29641 cagataacgg tcgaaagcgg cttccagcgc atcggaaatt tcacccaggg tggcgcgaac
    29701 gcgagcggca ttaacagcgg cagccagcag gttttcgtta tgctgtgcgg cgtgagtcag
    29761 ggcgttcaac gcggcggtta cggcggcatc atcacgggtg gcgcgaatgc gttccagcga
    29821 agcaatttgc tcgttacgca ccatcacgtt gtcgatctca agtacatcgg tttcgtcttc
    29881 gtgatccagt ttgtacttgt tgacaccaac gatgacacgc ttgccctggt cgatcagcga
    29941 ctgttcgcgc gctgaggcct cttcgatcat tcgttttggc agacctgctt cgatcgcttt
    30001 cgccatgcca ccggcttcgt cgatctgttg gataatagct ctggcttgtt tgacgatttg
    30061 atcggtcagc gactcaatgt aataggatcc ggccagtgga tcgacggtgc ggcagagttc
    30121 tgattcttcc tggatgatga tctgggtgtt gcgggcaatg cgtgctgaga aatcggtagg
    30181 caaaccaagc gcttcgtcaa aggcgttggt atgcagtgac tgagtaccgc ccagcgtcgc
    30241 agccagcgct tcaatggtgg tgcggataac gttgttatac ggatcctgtt cagtcaggct
    30301 ccagcctgag gtctggcagt gggtacgcag cgccagtgat ttcgggtcct gtgcgccaaa
    30361 tccactgacc gcttcgctcc ataaataacg tgccgcacgc aacatggcga cgttcataaa
    30421 cagatccatg ccgatgccga agaagaacga caggcgagga gcgaagtcat caattttcag
    30481 tccggcagag attgctgctt tgatgtactc aatcccatca gcgagcgtaa atgctacctg
    30541 ctgcacgcag ttggcacccg cttcacccat gtggtaaccg ctgatactga tggtattaaa
    30601 tcgcggcatg ttgccggaac accaggcgat gatgtcggcg ataatgcgca ttgacggttt
    30661 tggcgggtaa atataggtgt tgcggcagag gtactctttg agaatatcgt tttgaatggt
    30721 gccggtcagt ttatcaggtg taacaccttg ctcttccgcg gcgatgatat aaaacgccag
    30781 tactggtagc actgcgccat tcatggtcat cgaaaccgac attttatcca gcgggatctg
    30841 gtcgaacagg actttcatat cttccacggt gtcgatagcg acgcccgttt tgccgacgtc
    30901 acccgccacg cgtgggttat cggagtcgta gccacggtgg gtggcaaggt caaacgcaac
    30961 ggaaagacct ttttgcccgg cggccaggtt acggcgataa aaagcgttgg actcttttgc
    31021 tgttgaaaaa ccagcatact gacggatggt ccacggttgg gcggtataca tagtggcacg
    31081 cgggccacga acgtagggcg gcaaaccagg aagggtacct gtcacctcca gattatcgag
    31141 atcggcttcg gtatacagcg gcttgatggc gatcccttcc gcggtttgct gaaccagcga
    31201 gtcgacagtt ttctcccgac ggctcaattc cttgttggca agctgttgcc actcctgctc
    31261 gttagacatt atttgctccg taaaacgcaa tcaaaatgag ggtatttgcg cttacggtga
    31321 gctttttacg tgggtttgtc gccagcaaaa aaaatgcatt cagacgtcgt tttttgccgc
    31381 ctgagatttg tctattttgt gatgtgattc aaggaataca aaaagaagtg aggataacgc
    31441 ctgatatgtg ctatcaggca ttatttgatg gattaatcct gacgaaggac tttgtgacca
    31501 taatcgagca acgcatcagt gactttacgc atcatgcggc tttcaggagc aaagcggtgc
    31561 cagtagagca tccggcgttg aaatagccct ggcgttaagt caatcagttc accgctggct
    31621 agttctttct cgatttgcag gtgcgggatc atacagcagg tggtgccctg gcgagcaagt
    31681 tgcacgaacg cttctgaaga attaacgata tggcagggca cgctgcctgg aggcagatcg
    31741 aagttttgct gcaaaaaggc ctggtgcata tcgtcaagat ggtcaaacgc gaccactggc
    31801 gctttcagta atgccgaacg cgttacgccg ttagggaaat atttttcggc aaagggtttt
    31861 gagctgacga acagatagtc gagcgcacca agtttatcga caagacaact cggcagcgcc
    31921 tgatgttgaa tactcaccgc gccgaccact tcgccgcgac gcagacgttc ctgagtgcgg
    31981 gtttcatctt ctacctgcaa gttgaggcgg ataggcgaat cagccaatac aggagccagt
    32041 gccggaagca accacgtcgc cagactgtcg gcattgaccg ccagtgaaag cagcagcgga
    32101 gtcgaaccgg tttgttcatc gcccagccac tcttcttcca gcaactccac ctggcgcagc
    32161 agtgccagca gtttttgccc ttgttccgtc gggcgcggcg gtacggtacg caccaacagc
    32221 ggctgcccga acatattttc cagttgctta atgcgctgtg agacggctga ttgtgtaatg
    32281 cacagctttt gtgccgcgcg ctcaaatcct cgttcacgta tcaccgcatc cagtgcctgt
    32341 aatgttctgt agtccgggcg tttcattgct gttgtctgct cctggtaaat gctttttcct
    32401 gcactatgac acaattttgt gtcaggttgc atataccgca gccgtcggcg ggtaacattt
    32461 gtatcaatgg ttaaacgcgc agcttaaaag atcagggatt tgcgctattg catttcgctt
    32521 agcaacttcg cgccctggtt atgttgaatg gcgtggcgtt attgcctctg ttcgcaccgt
    32581 ggagcggcat acccagaccg ttgtatgctt tatttgcggg gcgaaagggg atgcccgcca
    32641 ttgcgcgata ttttatggat gagttaacca cgcggcttgc caacggggtc tgaatcgctt
    32701 tttttgtata taatgcgtgt gaaatttcat accacaggcg aaacgatcat gacgcaggat
    32761 gaattgaaaa aagcagtagg atgggcggca cttcagtatg ttcagcccgg caccattgtt
    32821 ggtgtaggta caggttccac cgccgcacac tttattgacg cgctcggtac aatgaaaggc
    32881 cagattgaag gggccgtttc cagttcagat gcttccactg aaaaactgaa aagcctcggc
    32941 attcacgttt ttgatctcaa cgaagtcgac agccttggca tctacgttga tggcgcagat
    33001 gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg cggcgctgac ccgtgaaaaa
    33061 atcattgctt cggttgcaga aaaatttatc tgtattgcag acgcttccaa gcaggttgat
    33121 attctgggta aattcccgct gccagtagaa gttatcccga tggcacgtag tgcagtggcg
    33181 cgccagctgg tgaaactggg cggtcgtccg gaataccgtc agggcgtagt gaccgataac
    33241 ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg acccgatagc gatggaaaac
    33301 gccataaatg cgattcctgg cgtggtgact gttggcttgt ttgctaaccg tggcgcggac
    33361 gttgcgctga ttggcacacc tgacggtgtc aaaaccattg tgaaatgatc tgacggggga
    33421 acctcccccg ttaaaaaaat tctcttcatt aaatttggtg acatgtgtca cgtttttacc
    33481 tggatattgt cgattgctct aaataaatcc tctaaaccag catattcaac caagaaatac
    33541 ctttgcgtga tatttcctca acatcgcgac gcaaacgttc atattgccgc aatattattt
    33601 tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca tttcaaagac aggattgggt
    33661 aaatggcaaa ggtatcgctg gagaaagaca agattaagtt tctgctggta gaaggcgtgc
    33721 accaaaaggc gctggaaagc cttcgtgcag ctggttacac caacatcgaa tttcacaaag
    33781 gcgcgctgga tgatgaacaa ttaaaagaat ccatccgcga tgcccacttc atcggcctgc
    33841 gatcccgtac ccatctgact gaagacgtga tcaacgccgc agaaaagctg gtcgctattg
    33901 gctgtttctg tataggaaca aaccaggttg atctggatgc ggcggcaaag cgcgggatcc
    33961 cggtatttaa cgcaccgttc tcaaatacgc gctctgttgc ggagctggtg attggcgaac
    34021 tgctgctgct attgcgcggc gtgccggaag ccaatgctaa agcgcaccgt ggcgtgtgga
    34081 acaaactggc ggcgggttct tttgaagcgc gcggcaaaaa gctgggtatc atcggctacg
    34141 gtcatattgg tacgcaattg ggcattctgg ctgaatcgct gggaatgtat gtttactttt
    34201 atgatattga aaacaaactg ccgctgggca acgccactca ggtacagcat ctttctgacc
    34261 tgctgaatat gagcgatgtg gtgagtctgc atgtaccaga gaatccgtcc accaaaaata
    34321 tgatgggcgc gaaagagatt tcgctaatga agcccggctc gctgctgatt aatgcctcgc
    34381 gcggtactgt ggtggatatt ccggcgctgt gtgatgcgct ggcgagcaaa catctggcgg
    34441 gggcggcaat cgacgtattc ccgacggaac cggcgaccaa tagcgatcca tttacctctc
    34501 cactgtgtga attcgacaac gtccttctga cgccacacat tggcggttcg actcaggaag
    34561 cgcaggagaa tatcggcctg gaagttgcgg gtaaattgat caagtattct gacaatggct
    34621 caacgctctc tgcggtgaac ttcccggaag tctcgctgcc actgcatggt ggtcgtcgtc
    34681 tgatgcacat ccacgaaaac cgtccgggcg tgctaactgc gctgaacaaa atcttcgccg
    34741 agcagggcgt caacatcgcc gcgcaatatc tacaaacttc cgcccagatg ggttatgtag
    34801 ttattgatat tgaagctgac gaagacgttg ccgaaaaagc gctgcaggca atgaaagcta
    34861 ttccgggtac cattcgcgcc cgtctgctgt actaattccc cttctctgaa aattaacggg
    34921 caggtcactg acttgcccgt ttttttatcc cttctccaca ccgttgccgc gttgcgcatt
    34981 ctactttgcg agtctgctcc agaaaataaa tcaaagtcag atcaatgcgt tgtgtttatt
    35041 taatggggat ttacgatggc gggacagcat ggggctgtaa cggtgaagcc ctcaccgaag
    35101 cgagggctta aaggagaagg gttatgatgc gacttgtcat catactgatt gtactgttac
    35161 tgataagttt cggagcttat taacagtcag tctcagggga ggagtcatcc tcccttaccc
    35221 ttactcacta aattaggtca aagaatcaac gatgtcaatc agggcgatgc ggggtgcatc
    35281 gccctgaacc gaactttttg atagccactt catcgcccac gtttactgcc gtaattaatc
    35341 ccttattcct gcaatgtatg tctgccttac atgacgtttt acgagtgcgc tcgaagaaaa
    35401 gatttaaagg tagatcaatg cgttgtggtt atttaaagag agtttacgat ggcagggcag
    35461 catggggctg taacggtaaa gccctcaccg aagcgagggc ttgaaggaga agggttatga
    35521 tgcgacttgt catcatactg attgtactgt tactgataag tttgccagct tattaacagt
    35581 cagtctcagg ggaggagtca tcctccctta cccttactca ctaaattagg ttaaagaatc
    35641 aacgatgtca atcagggcga tgcaggtgta tcgcccttac cactcccaga ctttcgacgg
    35701 tgtaaccacc gcaggaagag ggatatccca ctcttcaacg gggagttttt ccaccaactg
    35761 acaatcatgc gcataaccca ccggttgcgt tttatagtgc tgccagtttt gtaaggtccg
    35821 atcataaaaa ccaccgccca ttcccaggcg ctgaccgtac tcatcaaagg cgaccagcgg
    35881 tgtgatcagc acgtctaatc gggaaagggg gagcacgtca cgcacatcca gttttggctc
    35941 atggattttc aacctgttca tcaccagttc gctttgcgga tggtaattca ggaacagcaa
    36001 attaccggca ctaaagggat gcaaaactgg aagatatacg cgcttaccgg cgcgccagag
    36061 ttgttctata agcggctggg tgtcgagttc accatcaaaa gagaggaata cagcgaccgt
    36121 atgcgccatc accaccgggg gataagtcat catccgggta gcggcttgtt gacccatttc
    36181 ctgctgttgt tccggcgtta acgcacgacg acgttgccga atcattttgc ggatttcttg
    36241 tcgggataat gtcagtggga gttctggtag ttgcgtcatg gctggtgcca gataagaagg
    36301 gaatctccga gatgccgccg caggctgtaa cccttgaacc cttggttcaa ggtgaatgtg
    36361 tcgtcgcagt tttaaggctt ctcggacgga ccgagcatgc tcaccaaccg cggagcgcca
    36421 cattcttgtg gtatgaaata tcggctcagg ggactggccc gcttgcgaac atctcagaga
    36481 aattttgtct tcacggttac tctaccacag taaaccgaaa agtgtcattc aaagttttgg
    36541 ttagtttttt cggtgatgcg accttgttca agtaacgctt gttctatggt ctgctgcagc
    36601 atccgaatac gctgttccat acttgccgcg tagtcacgag tcttcgcttt ttcttgcgct
    36661 aactcatagc tgatattcaa tgcggcaatg aagaccaact gttcagtatt tgtgactcta
    36721 gtgcgttctt tcagatcttg caaccgttgg ttcagatcgt ccgctgcctg attcaacgca
    36781 tccctttggt caggcgggca gttcacacgc agtgaacggc caaaaatttg gatatcgacg
    36841 ggttgtgcag acatgccacc ttcctgctga ttgactgcgc tgccttcgtc tttagaccca
    36901 tggtctgcga aggggcgaca ctatagctac cctgatgaga agagacaagc ccttttctgg
    36961 tccaccaggg cccaaagtgg tagcatatca tgaatattcc tccctttgac gacgaatgct
    37021 tatgtctata cagaacgaaa tgcctggtta caacgaaatg aaccagtatc tgaaccaaca
    37081 agggacgggt ctgacccctg ctgagatgca tggtttaatc agtgggatga tatgtggcgg
    37141 taacgatgac agctcatggc taccgctact tcacgacctg acgaacgaag gcatggcttt
    37201 cggtcatgag ctggcacagg cattgcgtaa aatgcactct gccaccagcg atgccctgca
    37261 ggatgacggc ttcctttttc agctttatct gcctgatggc gatgatgtca gcgttttcga
    37321 tcgggctgat gcattggcag gttgggtcaa tcacttcctg cttggtcttg gtgttacgca
    37381 accgaagctg gataaagtga ccggcgaaac cggtgaagct atcgacgatc tgcgtaacat
    37441 tgcgcaactg ggttacgacg aagacgaaga tcaggaagag cttgaaatgt cgcttgaaga
    37501 gatcatcgaa tacgttcgtg ttgccgcgct gttatgccac gacaccttta ctcatccgca
    37561 accgaccgcg ccagaagtac aaaaaccgac tctacactaa aaacaaaaaa cgtaaggaga
    37621 gtgttatgag tgagatatcc cggcaagagt ttcagcgtcg ccgtcaggcc ctggtggagc
    37681 aaatgcaacc cggcagcgcc gcgctgattt ttgctgcacc agaagtaaca cgtagcgccg
    37741 acagcgaata cccctatcgt cagaacagtg acttctggta cttcaccggc tttaacgaac
    37801 cggaagcggt gttggtgctg attaaaagcg atgacactca taaccacagc gttctgttta
    37861 accgcgttcg cgacctgacg gcggagatct ggtttggccg tcgcttaggc caggatgccg
    37921 cgccagagaa actgggcgtt gaccgcgcac tggcattcag cgaaattaat cagcaacttt
    37981 atcaactact taatggtctg gatgtggttt accatgctca gggcgaatat gcatatgctg
    38041 atgaaatcgt gaacagtgcg ctggaaaaac tgcgtaaagg ttcgcggcaa aatctcaccg
    38101 caccggcaac gatgatcgac tggcgtcctg ttgttcatga aatgcgcctg ttcaaatcgc
    38161 cagaagagat tgccgtactc cgccgcgcgg gagaaatcac cgccatggca catacacggg
    38221 cgatggaaaa atgccgtccg ggaatgttcg agtaccatct ggaaggcgaa attcaccacg
    38281 aatttaaccg ccacggtgcg cgctatccgt cctataacac cattgtaggc agcggtgaaa
    38341 acggctgcat tttacactac accgaaaacg agtgtgaaat gcgcgacggc gacctggtgt
    38401 tgattgacgc aggctgcgaa tacaaaggtt acgcaggcga tatcacccgt accttcccgg
    38461 tcaacggcaa attcacccag gcccagcgcg aaatctacga cattgtgctg gagtctcttg
    38521 aaaccagcct gcgcctgtat cgtccgggaa cctccattct ggaagtcacc ggtgaagtgg
    38581 tgcgcattat ggttagcggc ctggtaaaac tcggcatcct gaaaggtgat attgatgaac
    38641 tgatcgctca gaacgcccat cgtcctttct ttatgcatgg ccttagccac tggttaggac
    38701 tggatgtcca tgacgttggc gtttatggtc aggatcgctc gcgcattctg gaaccgggga
    38761 tggtactgac cgtagagcct ggtctgtata tcgcgccgga tgcggatgtg ccagaacaat
    38821 atcgcggtat cggcattcgt attgaagacg acattgtgat taccgaaacc ggcaacgaaa
    38881 acctcaccgc cagcgtggtg aaaaagccgg aagaaatcga agcgttgatg gctgctgcga
    38941 gaaagcaatg agcgtaatca tcgtcggtgg cggcatggcg ggcgcgacgc tggcgctggc
    39001 tatttcccgg ttaagtcacg gggcgctgcc ggtacatttg attgaagcga ctgcgccaga
    39061 gtcacatgct catccgggct ttgatggacg agcgatagcg ctggcggcgg gtacctgtca
    39121 gcaactggcg cgcatcggcg tttggcaatc tctggcggat tgcgcaactg ccatcaccac
    39181 cgtgcatgtc agcgatcgag gtcacgctgg atttgtcacc ctcgccgcag aagattacca
    39241 actggcggcg ctgggacagg ttgtcgaatt gcacaatgtc gggcaacggc tgtttgcatt
    39301 gctgcgtaaa gcacctggcg taacgctgca ttgccctgat cgcgtggcta acgttgcccg
    39361 tactcagagt cacgttgaag tgacgctgga gggtggcgag acgctgacgg gccgcgtgct
    39421 ggtagccgct gatggcaccc attcagcgtt agccactgtg tgcggcgttg actggcagca
    39481 ggagccttac gaacaactgg ctgtgattgc caacgttgcg acttccgttg cgcatgaagg
    39541 gcgcgctttt gaacgcttta cgcaacatgg cccgctggcg atgttgccga tgtctgacgg
    39601 acgctgttcg ctggtctggt gtcatccact ggaacggcgc gaagaggtgc tgtcgtggag
    39661 cgacgagaag ttttgccgtg aactccagtc ggcctttggc tggcgacttg ggaaaattac
    39721 ccacgctggt aaacgcagtg cttatccgct ggcgttaacc cacgccgcca gatctattac
    39781 ccatcgtacc gtgctggtgg gcaatgcggc gcaaaccctg cacccgattg ccgggcaagg
    39841 gttcaacctc ggtatgcgag atgtcatgag tcttgcggaa accctgactc aggcgcagga
    39901 gcgcggagaa gacatgggtg attacggcgt attgtgtcgt tatcagcagc gtcgacagag
    39961 cgatcgcgaa gcaaccattg gcgtcacgga cagccttgta catctttttg ccaaccgttg
    40021 gacgccactg gttgtcgggc gcaacatcgg gctgatgacg atggaattat tcaccccggc
    40081 acgcgatgtg ctggcgcagc gcaccctcgg ttgggtggcg cgttgaaggc ggttattaag
    40141 gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt ggggctggcg
    40201 gttgcctgcg gcttacaggg tagcggctta cgcgttgccg tactggagca gcgcgtaccg
    40261 gaacctctgg cggcggatgc accaccacaa ctgcgcgttt cggctatcaa tgccgccagc
    40321 gaaaaattac tcacccgtct tggcgtctgg caggacattc tctctcgccg agccagctgt
    40381 tatcacggta tggaagtgtg ggacaaagac agttttggtc acatctcgtt tgacgatcaa
    40441 agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca ctacgcgctg
    40501 tggaacaaag cgcagcagtc gtcagatatc actcttttgg cccccgcaga attacagcag
    40561 gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagtat gttaactgcg
    40621 cgtctggtga ttggcgcgga cggcgctaat tcctggttgc gcaacaaagc cgatattccg
    40681 ctgactttct gggattatca gcatcacgcg ctggtagcga ccatacgcac ggaagaaccg
    40741 catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt tttaccgctt
    40801 agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc gcagcggatg
    40861 cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga taatcgcctg
    40921 ggcttatgca aggttgagag cgcgcgtcag gtgttcccac tgacggggcg ttatgcgcgc
    40981 cagtttgccg cgcaccgtct ggcgctggtg ggcgacgccg cgcataccat tcacccgctg
    41041 gcggggcagg gggtaaatct tggctttatg gatgctgcag agctgattgc tgaactgaaa
    41101 cggttgcatc gtcagggtaa agacatcggg cagtacattt atctgcgtcg ctatgagcgt
    41161 agccgcaagc acagtgcggc gctgatgctg gctggtatgc agggattccg cgatctgttt
    41221 tctggtacca atccggtgaa aaaactgctg cgtgatattg gtctgaaact ggccgacacg
    41281 cttcctggcg ttaaaccaca acttatccgc caggcaatgg gattaaacga tttgcctgaa
    41341 tggctgcgtt aaaaatttct cttctgctgt ttatttgata cccatcacac tttcatctcc
    41401 cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc tccgttttcg
    41461 cattatattt tctaatgtca cgaatttttg atttagtgtt ttttgacatt ttttcagctc
    41521 ttaatattgc
//
LOCUS       DAZKIF010000025        41207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZKIF010000025 DAZKIF010000000
VERSION     DAZKIF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.025"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..838
                     /gene="espX2"
                     /locus_tag="ACHOTW_001412"
     CDS             <1..838
                     /gene="espX2"
                     /locus_tag="ACHOTW_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFP9843549.1"
                     /translation="LNFNEQQGLLDKDNKCYILLSSDNSGRVMRLSHQALISMLEPEV
                     KKKTIWNNYSIYPSLQDTHEVVRDDPETICMRAFPLFAKGWEYAQKNKKHQLILNALG
                     FKGYIRDIFMSAIMRKTDFVLECNNQPTELNSSFSSLMNDSDQWQQHTLKDKHYANLL
                     TMLDLNDASESDKSKIFFCLSAVFANISHSNVFNGIPDASKTLKRYAFALLAKAHSLD
                     ESMISNQTFNTYKTVLLDFNNLSNEEANQLRISSLYRDMVRYAQYRFSKVLSEWTPDA
                     WL"
     gene            1122..3272
                     /gene="dinG"
                     /locus_tag="ACHOTW_001413"
     CDS             1122..3272
                     /gene="dinG"
                     /locus_tag="ACHOTW_001413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP9843550.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3300..4262
                     /gene="ybiB"
                     /locus_tag="ACHOTW_001414"
     CDS             3300..4262
                     /gene="ybiB"
                     /locus_tag="ACHOTW_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP9843551.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4403..5488
                     /gene="hcxB"
                     /locus_tag="ACHOTW_001415"
     CDS             4403..5488
                     /gene="hcxB"
                     /locus_tag="ACHOTW_001415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP9843552.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5555..5614
                     /gene="ybiE"
                     /locus_tag="ACHOTW_001416"
     CDS             5555..5614
                     /gene="ybiE"
                     /locus_tag="ACHOTW_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFP9843553.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5717..5977)
                     /gene="ybiJ"
                     /locus_tag="ACHOTW_001417"
     CDS             complement(5717..5977)
                     /gene="ybiJ"
                     /locus_tag="ACHOTW_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP9843554.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6242..6508)
                     /gene="ybiI"
                     /locus_tag="ACHOTW_001418"
     CDS             complement(6242..6508)
                     /gene="ybiI"
                     /locus_tag="ACHOTW_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP9843555.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLR"
     gene            complement(6584..7270)
                     /gene="ybiX"
                     /locus_tag="ACHOTW_001419"
     CDS             complement(6584..7270)
                     /gene="ybiX"
                     /locus_tag="ACHOTW_001419"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP9843556.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            complement(7312..9594)
                     /gene="fiu"
                     /locus_tag="ACHOTW_001420"
     CDS             complement(7312..9594)
                     /gene="fiu"
                     /locus_tag="ACHOTW_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP9843557.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDTIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9859..10119)
                     /gene="mcbA"
                     /locus_tag="ACHOTW_001421"
     CDS             complement(9859..10119)
                     /gene="mcbA"
                     /locus_tag="ACHOTW_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP9843558.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10395..11321
                     /gene="rlmF"
                     /locus_tag="ACHOTW_001422"
     CDS             10395..11321
                     /gene="rlmF"
                     /locus_tag="ACHOTW_001422"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP9843559.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(11318..13543)
                     /gene="ybiO"
                     /locus_tag="ACHOTW_001423"
     CDS             complement(11318..13543)
                     /gene="ybiO"
                     /locus_tag="ACHOTW_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP9843560.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNMAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13660..14382)
                     /gene="glnQ"
                     /locus_tag="ACHOTW_001424"
     CDS             complement(13660..14382)
                     /gene="glnQ"
                     /locus_tag="ACHOTW_001424"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP9843561.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(14379..15038)
                     /gene="glnP"
                     /locus_tag="ACHOTW_001425"
     CDS             complement(14379..15038)
                     /gene="glnP"
                     /locus_tag="ACHOTW_001425"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP9843562.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(15176..15922)
                     /gene="glnH"
                     /locus_tag="ACHOTW_001426"
     CDS             complement(15176..15922)
                     /gene="glnH"
                     /locus_tag="ACHOTW_001426"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP9843563.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(16326..16829)
                     /gene="dps"
                     /locus_tag="ACHOTW_001427"
                     /gene_synonym="pexB"
     CDS             complement(16326..16829)
                     /gene="dps"
                     /locus_tag="ACHOTW_001427"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP9843564.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(17128..18015)
                     /gene="rhtA"
                     /locus_tag="ACHOTW_001428"
     CDS             complement(17128..18015)
                     /gene="rhtA"
                     /locus_tag="ACHOTW_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP9843565.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            18250..18315
                     /locus_tag="ACHOTW_001429"
     CDS             18250..18315
                     /locus_tag="ACHOTW_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843566.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            18368..18883
                     /gene="ompX"
                     /locus_tag="ACHOTW_001430"
     CDS             18368..18883
                     /gene="ompX"
                     /locus_tag="ACHOTW_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP9843567.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18932..20515)
                     /gene="opgE"
                     /locus_tag="ACHOTW_001431"
     CDS             complement(18932..20515)
                     /gene="opgE"
                     /locus_tag="ACHOTW_001431"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9843568.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20787..20915)
                     /gene="mntS"
                     /locus_tag="ACHOTW_001432"
     CDS             complement(20787..20915)
                     /gene="mntS"
                     /locus_tag="ACHOTW_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP9843569.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21101..21568
                     /gene="mntR"
                     /locus_tag="ACHOTW_001433"
     CDS             21101..21568
                     /gene="mntR"
                     /locus_tag="ACHOTW_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP9843570.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21565..22683
                     /gene="ybiR"
                     /locus_tag="ACHOTW_001434"
     CDS             21565..22683
                     /gene="ybiR"
                     /locus_tag="ACHOTW_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP9843571.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(22741..23661)
                     /gene="ldtB"
                     /locus_tag="ACHOTW_001435"
     CDS             complement(22741..23661)
                     /gene="ldtB"
                     /locus_tag="ACHOTW_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9843572.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVLVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23880..25472
                     /gene="ybiT"
                     /locus_tag="ACHOTW_001436"
     CDS             23880..25472
                     /gene="ybiT"
                     /locus_tag="ACHOTW_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP9843573.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(25640..26905)
                     /gene="ybiU"
                     /locus_tag="ACHOTW_001437"
     CDS             complement(25640..26905)
                     /gene="ybiU"
                     /locus_tag="ACHOTW_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP9843574.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(27057..27872)
                     /gene="ybiV"
                     /locus_tag="ACHOTW_001438"
     CDS             complement(27057..27872)
                     /gene="ybiV"
                     /locus_tag="ACHOTW_001438"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP9843575.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(28018..30449)
                     /gene="ybiW"
                     /locus_tag="ACHOTW_001439"
                     /pseudo
     CDS             complement(28018..30449)
                     /gene="ybiW"
                     /locus_tag="ACHOTW_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            complement(30455..31354)
                     /gene="ybiY"
                     /locus_tag="ACHOTW_001440"
     CDS             complement(30455..31354)
                     /gene="ybiY"
                     /locus_tag="ACHOTW_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP9843576.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            31485..32147
                     /gene="fsa"
                     /locus_tag="ACHOTW_001441"
     CDS             31485..32147
                     /gene="fsa"
                     /locus_tag="ACHOTW_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9843577.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(32326..33075)
                     /gene="moeB"
                     /locus_tag="ACHOTW_001442"
     CDS             complement(32326..33075)
                     /gene="moeB"
                     /locus_tag="ACHOTW_001442"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP9843578.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(33075..34310)
                     /gene="moeA"
                     /locus_tag="ACHOTW_001443"
     CDS             complement(33075..34310)
                     /gene="moeA"
                     /locus_tag="ACHOTW_001443"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP9843579.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            34514..35479
                     /gene="iaaA"
                     /locus_tag="ACHOTW_001444"
                     /pseudo
     CDS             34514..35479
                     /gene="iaaA"
                     /locus_tag="ACHOTW_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            35466..37337
                     /gene="gsiA"
                     /locus_tag="ACHOTW_001445"
     CDS             35466..37337
                     /gene="gsiA"
                     /locus_tag="ACHOTW_001445"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP9843580.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            37357..38895
                     /gene="gsiB"
                     /locus_tag="ACHOTW_001446"
     CDS             37357..38895
                     /gene="gsiB"
                     /locus_tag="ACHOTW_001446"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP9843581.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            38913..39833
                     /gene="gsiC"
                     /locus_tag="ACHOTW_001447"
     CDS             38913..39833
                     /gene="gsiC"
                     /locus_tag="ACHOTW_001447"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP9843582.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            39836..40747
                     /gene="gsiD"
                     /locus_tag="ACHOTW_001448"
     CDS             39836..40747
                     /gene="gsiD"
                     /locus_tag="ACHOTW_001448"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP9843583.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            40925..>41207
                     /locus_tag="ACHOTW_001449"
     CDS             40925..>41207
                     /locus_tag="ACHOTW_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFP9843584.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPF"
BASE COUNT         9851 a        10197 c        11082 g        10077 t
ORIGIN      
        1 attgaatttc aatgagcagc aaggattgtt agataaagat aataaatgtt atatcctgtt
       61 atcatccgat aactctgggc gagtaatgcg cttatcgcac caggcgttaa tctcaatgct
      121 tgagccagag gttaagaaaa agactatctg gaataattac tctatttatc catctttgca
      181 ggatacccat gaagttgtca gagatgatcc agaaacgatt tgtatgcgtg catttccttt
      241 atttgcaaaa ggatgggaat atgcgcagaa aaataagaaa catcaattga tcctcaatgc
      301 tcttggattc aaaggttata ttcgtgatat atttatgtca gcaataatgc gaaaaacaga
      361 ttttgtgctc gaatgcaata atcaaccgac agaacttaat tcatcatttt cctctttgat
      421 gaacgattca gatcaatggc aacagcatac tctgaaagat aaacattacg ctaatctatt
      481 aacgatgctg gatctcaatg atgccagtga aagtgataag tctaaaatat ttttctgcct
      541 gtctgccgta tttgcaaata tttctcacag taatgtcttt aacggtattc ctgatgcgtc
      601 aaaaacttta aaacgttatg catttgcttt attggcgaaa gctcattctt tagatgaaag
      661 tatgataagc aatcaaactt ttaatacata caaaacggta ttgcttgatt tcaataatct
      721 gtctaatgaa gaggccaacc agttgcgcat aagttcgtta tatcgtgata tggtccgcta
      781 tgcacaatat cgtttttcta aagttttatc cgagtggacg ccagacgcat ggctgtaaag
      841 atcgcagcag aataatggca ttgatggccc tggctcaggc cagggtcggc aataccggtt
      901 cctgcgaagc gatgacggcg gcaattgtaa ccacgaaatg cggtatcact tgctgttttc
      961 tgccctgaag actggcgctc actgtgccgt cgaattttca atcaaatata ttgcgagagg
     1021 gcgtgtttgc gagccgcttt ccagaaacag aaaaaccatt acccctgaaa accgaaaaat
     1081 gccacaatat tggctgttta tacagtattt caggttttct catggcatta accgccgcgc
     1141 ttaaagcgca aattgccgcc tggtataagg cgcttcagga acagatcccc gactttattc
     1201 cccgtgcgcc gcagcggcag atgattgcgg acgtcgccaa aacgctggcc ggagaagaag
     1261 ggcggcatct ggcgattgaa gcccccactg gcgttgggaa aacgctctcc tatttgattc
     1321 ccggcatcgc cattgcccgc gaagagcaaa aaacgctggt ggtgagtacc gccaacgtgg
     1381 cattgcagga tcagatctac agcaaagatt tgccactgct gaaaaagatc attcccgatc
     1441 ttaaattcac tgccgctttt gggcgtgggc gctacgtttg cccgcgtaat ctgacggcgc
     1501 ttgccagcac tgagcccacg caacaggatc tgctggcgtt tcttgacgac gaactgacgc
     1561 caaataatca ggaagagcaa aaacgttgtg cgaagctgaa gggcgatctc gacacttata
     1621 aatgggatgg tctgcgcgat catacggata tcgctataga tgacgatctc tggcgtcgtt
     1681 taagtaccga caaagccagc tgcctcaacc gcaactgtta ctactatcgc gaatgcccgt
     1741 tttttgtcgc tcgtcgggaa attcaggaag cggaagtggt ggtggcaaac catgcgctgg
     1801 tgatggcggc gatggaaagc gaagccgtat tgcctgaccc gaaaaattta ctgctggtgc
     1861 tggacgaagg ccatcacctg ccagatgtgg cgcgggatgc gctggagatg agtgccgaaa
     1921 tcaccgcgcc atggtatcgg ctacagctgg acttgttcac gaaactggtc gctacctgca
     1981 tggagcagtt tcgcccgaag accatcccgc cgttggcgat ccctgaacgt ttgaatgcgc
     2041 attgtgaaga gctgtatgag cttatcgcct cattaaacaa cattctcaat ctctacatgc
     2101 ctgccgggca ggaagcagaa caccgttttg cgatgggcga actgcctgat gaagtgctgg
     2161 agatctgcca gcggctggca aaactcaccg agatgctgcg tggtctggct gagttatttc
     2221 ttaacgattt aagtgagaaa accggcagcc atgacattgt acgtctgcat cggttgattt
     2281 tgcagatgaa ccgcgcgttg gggatgttcg aggcgcaaag caaactctgg cggctggctt
     2341 cgctggcgca atcttccggt gcaccggtga ccaaatgggc gacgcgggaa gagcgcgaag
     2401 ggcagctaca cctctggttt cactgcgtgg gaatacgtgt cagcgatcag ctggaaaggc
     2461 tgctgtggcg cagtattccg cacattattg tcacctccgc aaccttgcgt tcgctgaaca
     2521 gtttttcgcg tttgcaggag atgagcgggc tgaaagagaa agcgggcgac cgttttgtgg
     2581 cgctggattc cccctttaac cactgcgaac agggcaaaat tgttattccc cggatgcgcg
     2641 ttgagccttc catcgacaac gaagaacagc atattgctga aatggcggcc tttttccgtg
     2701 agcaggtgga gagcaaaaaa catctcggta tgttggtgct gtttgccagc gggcgtgcga
     2761 tgcagcgctt tctcgactat gtgacggatt tacgtctgat gttgctggtg cagggcgatc
     2821 agccgcgtta ccgtttagtt gaactgcacc gcaaacgcgt cgccaacggt gagcgtagtg
     2881 tgctggtggg cttacagtca tttgccgaag ggcttgattt gaaaggtgat atgctcagcc
     2941 aggtgcatat ccacaaaatc gcttttccgc ctatcgacag cccggtggtg atcactgaag
     3001 gggaatggct gaaaagcctc aaccgctatc catttgaggt gcaaagcctg ccgagcgcct
     3061 cgtttaacct gattcagcag gttgggcgac tgattcgaag tcacggttgc tggggcgaag
     3121 tggtgattta cgataaacgc ttgctgacca aaaattatgg caagcgacta ctggatgcat
     3181 taccggtatt tccgatagag caaccggaag tccctgaagg tatagttaaa aagaaagaaa
     3241 aaacgaaatc cccacgccgt cggcggcgtt aatgatgtga gtcaggtaag gagtcgtaaa
     3301 tggactatcg caaaatcatt aaagagatcg ggcgcgggaa aaaccacgcg cgcgatttag
     3361 accgggatac tgcgcgcggt ctgtatgccc atatgctcaa tggtgaagtc cctgaccttg
     3421 agttgggcgg tgtattgatt gcgctgcgta tcaaagggga aggggaagca gagatgctcg
     3481 gcttttacga agccatgcaa aatcacacca tcaagctgac gccgcctgca ggcaagccga
     3541 tgccgattgt catccccagt tacaacggcg cgcgtaaaca ggccaacctg acgccgttgt
     3601 tggcgattct cctgcacaaa ctcggttttc ctgtggtggt tcacggggtt agcgaagatc
     3661 caactcgcgt gctgaccgaa accattttcg aactgatggg tattacgcca acgttgcacg
     3721 gcgggcaggc gcaggcgaag ctcgacgaac atcaaccggt gtttatgcct gtcggcgcgt
     3781 tttgcccgcc gctggaaaaa caactggcga tgcgctggcg gatgggcgtg cgtaacagtg
     3841 cacataccct ggcgaaactg gcgacgccat ttgccgaagg tgaggcactg cgtctttcca
     3901 gcgtttcgca tccggaatac atcgggcgag tcgcgaagtt ctttagcgat atcggcgggc
     3961 gggcgttact aatgcatggt actgaaggcg aagtgtatgc taatccgcag cgctgcccac
     4021 agatcaatct cattgatcgt gaagagatgc gggtgctgta tgaaaaacag gaccctgctg
     4081 gaagcgagtt actgccacaa gcaaaagatc cggaaaccac ggcgcagtgg attcagcgtt
     4141 gccttgctgg ccgcgaaccg attcccgaat cgctgaaaat ccagatggct tgctgcctgg
     4201 tggctacggg tgaagcggca actatcagcg atggcctggc gcgcgttaat caggcatttt
     4261 aattcttttc tcagccggat gagccatgtc atccggcttt taccccaccg caatatgaaa
     4321 ttcctgcatc tttattgacc ttcccgcgcc cggcgtgcag cataaaaaaa caacaaacac
     4381 ataacataaa caggagttaa ccatggaaag tggtcatcgc tttgatgctc agacgctgca
     4441 cagctttatt caggctgtat ttcgtcagat gggtagcgag gaacaagaag cgaaattagt
     4501 tgccgatcat ttaatcgcgg caaacctggc agggcatgat tcacatggta ttggcatgat
     4561 cccaagctat gtacgctcct ggagtcaggg gcacctgcaa attaaccatc atgccaaaat
     4621 cgttaaagag gcgggggcgg cggtcacgct cgatggcgat cgcgcatttg gtcaggtcgc
     4681 ggcacatgaa gcgatggcgc tggggattga gaaagcgcat cagcacggta ttgctgccgt
     4741 ggcgctacat aactcgcatc atatcggccg tatcggttac tgggcggagc agtgtgcagc
     4801 ggcggggttt gtctctatcc actttgttag cgtggtcggt attccaatgg tcgcaccgtt
     4861 ccacggtcgc gacagccgct ttggcacgaa tccgttctgt gtggttttcc cacgtaaaga
     4921 tgattttccg ctgttgcttg attacgccac cagcgccatt gcatttggca aaacccgcgt
     4981 cgcctggcat aaaggcgtcc ccgtgccgcc aggttgcctg attgacgtta acggcgtgcc
     5041 gacgaccaat ccggcggtaa tgcaggagtc gccgttgggt tcgctgttga cctttgccga
     5101 acataaaggc tacgccctgg cggcaatgtg tgaaattctt ggcggggcgc tttctggcgg
     5161 taaaacgacg catcaggaaa cgttacaaac cagtcccgat gccattctta actgcatgac
     5221 cactatcatc atcaacccgg aactgttcgg cgcgccggat tgtagcgcgc agaccgaagc
     5281 ctttgccgag tgggtgaaag cctcgccgca tgatgacgat aagccgattt tgctcccggg
     5341 cgagtgggaa gtgaacacgc gtcgcgaacg gcaggagcag gggattccat tggatgcggg
     5401 aagctggcag gccatttgtg atgcggcgcg gcagattggt atgccggaag agacgttgca
     5461 ggctttctgt cagcagttag ccagctaaaa aaaagcccgt ccagtggcgg acgggcaaac
     5521 aagggtaaca taggatcaat gagggttaga gcatatgcgt ctgtcggcaa acagacaggg
     5581 aaatacttgt gcaggacgta gcgtaaacgc ctgatccgtc caacggttcg gattttgtag
     5641 gcctgataag acgcggtagc gtcgcatcag gcatctgttg ccggatgcgg cgtaaacgcc
     5701 ttatccgtcc tacgaattac ttataaatta ccgcagtacc gcttaattta ttgttgttgg
     5761 tggcggaagt gatgctatat ccactggcac cggctgccgc cgctttctca gccagtttcg
     5821 cttccagggc atcgagggtg gatgcgccat cggcagaaac cacgccgatt ttattcatgt
     5881 tctgtgcctg ggatgccgtt accggttccg cggcaaacac gccaaatgac agagctgaaa
     5941 gagccatagc agcaacaaca gtattgatag ttttcataat taatctctcg caggtgattt
     6001 ttgtataagt gacgttgttt cgtcgatgtg atgagtatca cgttttttta cgagagataa
     6061 aatcgaagag aattgaccgc cttgttcaaa taaattgatt gatatctaac atactgataa
     6121 accatttatt tgttgtactt atggacttgt tttatgcagc gtggcagtca cgacagcgtt
     6181 aaaggcacgg aatgccacgc aaaatacaaa ttatactcgc tatacatctc gccagttgta
     6241 gttaacgtaa ctggctttct ttcgaacctc tgcgattata tcctgtataa gctggttttt
     6301 gtaaatcttt ctcctgctga caatgaatac ataagcgcac gccaggaatg gcttcccgac
     6361 gggcttgcgg gatgggggca ccgcactctt cacattcata caggctttcg ccacgcggaa
     6421 tttcaccccg agcgcgggca atcgcatctt caattgtact gttgatctgt tcgttgacgg
     6481 cgtcatcgtt agcccaaccg gatgccatat cgtcctcccc atatatcaat gcttgtatag
     6541 ttaagtgtag ctaatatagg gacgaacctg ggcagttcaa gcgtcagatc tccgaccatt
     6601 cccgcagcag attatgataa agattgagca gcgacaggat ctcttcattt tcaccatagc
     6661 ggcttttcag cgactgaata ttctgaatat ttttgtccag ttcaaacagc atggcgcgct
     6721 ttttatcatc gcggatcatc gactggatcc atataaatga tgccactcgt acgccgcggg
     6781 ttacgggtgt cacgcaatgc aggctgctgg agggatacaa caccagatca cctgccggga
     6841 gttttacccg atgttgtccg aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt
     6901 gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg catccagccg ttttgtggat
     6961 ggctgcgtac tgcgccatcc acatgaaaac cataggtttc attgttctga tagcgattaa
     7021 acagcggcgt ggaaagggta cgcggcaagg ccgcggcaaa gaacaaagca tgttggttaa
     7081 ccgcgttcag cacctcattt tgcagggcgg cgtataacgt gctgcgggtg tctacctgtt
     7141 gattgttctt aacttgcgca ccttgtgcgc cggtggtgac gcgtccatcc acccattcgg
     7201 cttgttccag ttgttcgcga aaacgggcga cgtcctgtgg cgataacacg ccgggaatgt
     7261 ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg
     7321 catattggct gtgagcaaga aggttcttgg ctcgcccggg tgataacggt agccgctctt
     7381 attgattgag gcgacgtaat cggtatcaaa caggttgtag acgtttagct ggaagtcgag
     7441 attgcgatta accctatacc ccagtttggc atcggcgacc cagtaacctt cggtaaacgc
     7501 tggcgttccc accgcgccgt ctgaaccttt atgcatactg ccgatatagc gtgcgcccgc
     7561 gccaacagag atatcatcgg ttgcctgata ttggctccat aaggtgaagg cgtgctccgg
     7621 ggtatacggc agcgatgagg aaccatcctg ggcaacatct ttgccgtttt tgatggttgc
     7681 tttttgctgg gtatagccgc caatcacctg ccacgcggga gtgatattcc ctgccacgga
     7741 tatctcatag ccttcgacgc gtttcttacc gtattgcgag taagtcccgt catcattttg
     7801 ctcaacttca ttttcgatat cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc
     7861 cagaacctgc catttggtgc caatctcgct ggtgttggct ttttgcggtt taaaatcggt
     7921 gcggttggca ctgttaccgc tgccagactg cgcaagggcg aagttgttgc cgcccggagg
     7981 ctgctgggaa acggcatagt taatatagac attgccgttt tccgtcaggt gatacagcgc
     8041 cccggctttc cagttcacca gattgcccga cttggcggtg tcgacggtgg tgaccggaga
     8101 accttttgcc acaccagctg ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggcggt
     8161 ggcactgtca tattcagtat gataattatc cagacggatc ccgccgttca gctcaaaatc
     8221 acgggtgatt tgcagcgtat cgaatgcgta aattgcgaag gtatccgtct gaccattggc
     8281 gtttgcgccg ttgcgcgtca ggccgccggg atgaatgctg ctgtcaggat gataaatatt
     8341 taccgcaggt aacgtcaccg gattaacgcc gtagttagtc tgcgtttcac gggtaaattc
     8401 cacgccggta ctgacatcat gaccgataga agcggtatag aatgtcgagg tcaggttggt
     8461 ctggttggtg agaattttat tactcacatc tttggtattc gccgtgcgtg accacgtcca
     8521 gctattcaca tcgctggtgg gctgggtaat attcgacgcc ccacccataa tcgccgtcat
     8581 caggtaatcc tgcttcacgc gcgaccaacg ggtagtgttg cgaatggtgg tgttatcgtt
     8641 gatatcgtgc tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc
     8701 cgtgccgtaa aagttatgcg tatcaacttt tccggaatga ttcagggtcg ccgttcctgc
     8761 agatggggca gaatagcccg gcaaaccgat ggttggaatg ccgccgtctg gcgtgttgtg
     8821 ctgggtgaca tgcagataat taagatacaa acgattcgct gtaccaaggc caaaagcgat
     8881 agaaggggcg acgccgtaac gctcattttt gactttgtcg cgtccggcat cgtgcgtttt
     8941 ttcgcccatc acattcaggc gcaccgcagt ggtatcacca atgacctgat tgacgtccag
     9001 cgtgccgcgg cggaaccagg cgctgccaat actggcggag gcgtcaatgc cggaatcatt
     9061 gcgcggctgc ttgctgatca tattgatcga gcccgtcggc gcgctgcgcc cgtagtcggt
     9121 gccggaaggc cctttaatca cttcgacctg ctcagtattg aaggtgtcgc gcgagacgct
     9181 gccgatatcg cgaatgccat caatataaat actgttagag gtatcagcac cgcgcatata
     9241 aatggtgtcg ccagtggtgg agttaccgtt ctcacccgca aaaaacgcac ccacgccggg
     9301 gacgtttttg agcgcatcgg taaggttggt tgcgccctga tctttaatca cttgttcaga
     9361 aatcaccgtc atcgtgcggg tagtatccgc taccggacgc gagaatttcg gatcggcaga
     9421 ttgttgtggc gcataaagcg aaggcgttga tgcttcgaca accagcgtgt catccgcgtt
     9481 agtttgccct tcggcggcga gtgcctgagc cacaggcgtg atgccaatac aaagaccggc
     9541 aaagaacgtg agcgaatgaa attgtctggc agggaaattg cgattgtttt ccattttgca
     9601 ggtgactttt tcttatatgt tgaaattttc actgccacga gaaagcctct ctgcggatgc
     9661 gatgccactg ttaatctcgt gatagtgttc gtgcaagtga taataatttt gataagcatt
     9721 atcacttggc gcgattttct atcacccatt gcgcgaaaaa gaaatacatt tatttacatt
     9781 gcgttcatat ttgatgtttt ttgattacaa aacaggcgaa aactgcatcc agaggcgaat
     9841 gcagttacgt tggtggggtt atttgtagat ggttgcagta ccgaacatct ggtcatttcc
     9901 gcctgcggaa ttaatgacat aacctttcgc gccttgctcg cgcgcttttt ctgccagttt
     9961 atcctccaga tcgcttaggt ttgatgcgcc agttgccgaa acggtaccgg cgggccgtag
    10021 ctgaccgctg tccaggttac tcatcggttg cgcggcataa gccgtgagag agattccgct
    10081 caggacggtg gcaatcagta gtgtgaggca ctttttcata atgacatcct cataaataca
    10141 acgggtagtg cctgataagt gtaggtcgcc tgcgcgatgc aaatgacgca aaaaattgac
    10201 agagatgtgc ttattttttg aacgatatat ttttacaaaa taagacaaat caatgacatg
    10261 cataatgctg atttttatga tgaattgcca taaaccatag ccatgttaag gttttgggga
    10321 atgataaacg cagccagata tttaccctgt cgcgttatgc gggtatctta cgccgctgct
    10381 taaaggagaa tgctatgtcc gcccagaaac cggggttgca tccgcgcaac cgtcatcaca
    10441 gccgctacga tctcgccacg ctttgtcagg tcaatcctga actcaagcaa ttcctcacgc
    10501 ttacacccgc cggggagcaa agcgtagact ttgccaatcc gctggcggtg aaggcgctca
    10561 ataaggcgtt gctggcccat ttttacgccg tagcgaactg ggatatcccg gacggttttc
    10621 tctgcccgcc agtaccgggc cgggcggatt atattcatca ccttgccgat ttactggctg
    10681 aagcaagcgg aacaattcca gctaatgcca gcattcttga tatcggcgtt ggtgcgaact
    10741 gtatttatcc gctgattggc gtacatgaat atggctggcg ttttaccggt agtgaaacca
    10801 gcagccaggc gttaagcagc gcgcaggcga tcatcagtgc taatccgggg cttaatcgcg
    10861 ccattcgtct gcgtcggcaa aaagagagtg gggcgatttt taacggcatc atccataaaa
    10921 acgagcaata cgacgcgacc ttgtgtaacc cgccattcca cgattccgcc gctgcggcgc
    10981 gggcaggtag tgagtgtaaa cgccgtaacc tggggctgaa caaagacgat gcactgaact
    11041 ttggcggcca gcaacaggag ttgtggtgtg aaggcggtga ggtcaccttt atcaaaaaga
    11101 tgattgaaga gagcaaaggc ttcgcgaagc aggtgatgtg gtttacttca ctggtatctc
    11161 gtggtgaaaa cttaccgccg ttgtatcgtg ccctgacgga cgtgggcgcg gtgaaggtgg
    11221 ttaaaaaaga gatggcccag gggcaaaagc agagtcgctt tattgcctgg acctttatga
    11281 acgacgagca gcgccgccgt tttgtcaatc gccagcgtta aagcgttggc tcccccggcg
    11341 gtaacggttc agccggggtc gcgcccggag caggcagcac ctgataagtc tgcaccggcg
    11401 cgcgaacgcc cgccaggtcg aaatgttttt tcacctggct gtcgagggca aagcgtaccg
    11461 tccactgttt gagtggcagc gtggtgaacg aaacacgcag tgtaaacgcg gtattgctta
    11521 agccaacaat cccggcaaag ttcggttcac caataatcag cccacgaatt tcttcgtttt
    11581 ccattaattc cgctaccgca tctttcagtg cctgattggc tttatcagca tcttcatggc
    11641 gatcaacatc gtagtttgcc actaccgaac caatgccgcg gacaaagttg gcaaaggtgg
    11701 taatcgaaga ccacggaatg atgtggtacg ccccggtatc ctggcgtacg cccacggagc
    11761 gaatcgacat ccgttccaca gtgccggtca acggcccaat agtcaccaaa tctccagtgt
    11821 tcatgccgtt ttcaaactga ataaataccc cggtgataat atctttcacc agcgtttgcg
    11881 aaccaaacga gatagccaga cctaatgccc cggcacctgc cagcaatggc gcgatattga
    11941 cgccgatttc cgacaacaca atcatgatgg tgatggtact gataatcacc gccagcgcgt
    12001 tacgaaacag cgtaagcagg gtacgcgtgc gggcgctggg tagcgggcgg ccatgaatat
    12061 ccgaagccag ccggttttcg atcaaactgg cgagcaccgt ccagccaacc gccgagaaga
    12121 aaagaatgag tgcgatacgg atcaggatat ctacggtttt ctgccccgcg ccgttttgca
    12181 gccagttcca gaaatcgaac aatccccatg cgctcaacag cagcattacc gccacgcaga
    12241 cggtcagaat acgcgccgtt ttcagcgccg ccgaaagcca gccattcagc cgtttttgca
    12301 actccggata gttacgctga gtatgtggcg agagagtgat ggtttttgcc agccagcggg
    12361 aaaacatacc ggaaacaaat gctgcgatac caataatcgc caggctgcgc actgttgcac
    12421 ccatcataaa tttcaggctg ttgcccggat cgaacaacga aaagaaaaac agcacgataa
    12481 aataggcgct tgccagccag tgccacacca gcgcaaaggc gcggataaac aggctgaaaa
    12541 aggccagcga atgctccgca aagttgagca aatgctgggt aatctctttt ttattacgaa
    12601 agatcaggta caacgcccag acggtcatgc acagcataat gatgacgttc gccagcgcac
    12661 ctatctgtac attcacctga ttagagataa tcggcacggc cacaatcagg ccataaccta
    12721 tcaaactgcc taaccagctc aggcgacgac tccagtaacg ggcgctctcg tcctgaatcg
    12781 tgaacgggcg cagttccgcc atgtttgggc aaaaaatcag gcgtagtacg gctttgaaaa
    12841 attcaatcag ggcaaaggcg ttgagaaaca aactttgttg gaaagcgatg gtgcgactgc
    12901 ctgcattcag gttgtcgctt aatatctggc cgacaaataa tgtcagtgcc agtaacagca
    12961 ggtcgataat aaacgccccg ataatcatcg ccggaagctg cagccagtta ctgcgctcac
    13021 gatttttttg ccgcgcccac tgacccattt tgcgatacag cggcagtgcg cacaggcgaa
    13081 tcagccagta aaaaccaaac actaaaaccg ctaacattga aaaatgggtc agcgcattgc
    13141 tgaaggtttg tggattaaac ggcttatgcg gggagccggt gatattgcga taaagctgcc
    13201 cgaagcgggc ggaaagggct tcaccataat ggcggctgaa ttcggtgacc ttttgcagca
    13261 cggtttgctc ttcaaccagc gtcggcggca cgatcttcgg gaccggttca gcaggtggcg
    13321 tcgcggcgac ggtgcgcaac tggtcgatca actctttacg cgaggtgtca ttatccagca
    13381 catccgccag tgcgccataa gccgcttttt tttgttcgat atccggttcc ggggccggtt
    13441 cagtcgttga gtccgttgtc gttgtggttg taacgccggg tatggatacc gcgtgggcag
    13501 gtgcgcccag caggcagaag aggatgaaca ggatccaccg catgactcct ccagtgagaa
    13561 aacagagcaa aaagataagt atagatgctg ggggaggggt gattttaaat gagaggaatc
    13621 tggtgtacct ccctttcggg tgaaagggag gcagagaagt taagagacgt gctgtaaaaa
    13681 ttcctgcaag cgctggctcg gcggattttt aatcaacact tgtggatcgc catcttccgc
    13741 aatccggcct ttgtcgataa agatcaggcg cgaagctact ttctcggcaa aaccgatttc
    13801 gtgggtcacg atcaccatcg tcatcccttc ttcagccaga tcttgcataa ccttcagcac
    13861 ttcatggcgc agttccgggt caagagcgga agtcggttca tcaaacagca tcattttcgg
    13921 cttcaccgcc agcgcgcggg caatcgccac acgctgctgt tgaccaccag aaagttcgga
    13981 agggtagtga tgtgcacgtt ctgccagacc gactttcgcc agcagctcac gtgccagttt
    14041 ttccgcctct tctttgttcg cgccacgcac gcgtagcggg ccaaacatga cgttttccag
    14101 cgccgtcaga tgcgggaaga ggtaaaactg ctggaacacc atacctgctt cctggcgaat
    14161 caggcgctcg tcaactttcg gatcgttaac cttcaggcca tcgacaatca gatcgccgga
    14221 ggtgatttct tccagtttgt tgatgcagcg cagcagggtc gatttaccgg aaccggacgg
    14281 cccgataatc accacgactt cgccctgggc aatgttcaaa tcgatattgt gcagcacctg
    14341 ggttgggcca aagtgcttgg agacgttttt aaattcaatc acaggatttt catccttctt
    14401 tccagacgac gcagaataaa gctcagcacc agggtaataa tcagatagaa caccgccacg
    14461 gcgctccaga tctcaagggc gcggaagtta ccggcaataa tttcttgccc ctgacgggtc
    14521 agttccgcca cgccgatcac aataaacagc gaggtgtctt taatgctgat gatccactgg
    14581 ttacccagcg gcggcagcat acgacgcagt gccagcggta aaatgacgta gcgaatggtt
    14641 tcccaacgtg aaagaccgag cgccagtcct gcttcacgaa aacctttgtg gatagacagc
    14701 accgcaccac gcgtgatttc cgcaatgtac gcgccggagt tgatcatgat ggtgaccacc
    14761 gccgcagtaa atgggtcgat gcgtaagtca ttaaacgcca tcggcagggc aaaatagata
    14821 aacatcacct ggacgacgat aggtgtgccg cggatcactt caataaaaac cagcgcgacg
    14881 tggttggcta tccaaccacc gaaggtgcgt gcaaaacctg ccagcaatcc gatcaccagg
    14941 ccgccagcca gaccgaggac cgatatccac agggtcattt tggcaccttc aatcagaagc
    15001 ggaatggcag gccagatagc actccagtca aactgcatat gttgttcctg ttaccgtggt
    15061 gtaaataatc aaattcaggg taattaatgg ccccggagcg ggtttgcgcc ctcaccctaa
    15121 tcctctcccc agaggggaga gggaactgcc agtgcgcttt acaggtgtag cgttattatt
    15181 tcggttcagt accgaaccat tttttgtaga tttcgttgta agttccgttc tcgcgcaggg
    15241 ttttcaacgc gccgttgact ttgtcacgca gctcgtcgct acctttcggg aacgcaatac
    15301 cgtattgctg cgcttccaga gagtcgccta ccgctttgaa ctgaccgtta ccggcggttt
    15361 tgatgaagta cagaatgttt ggcgtatcgt gcagaacggc gtctgcgcgg ttggtgccca
    15421 gttccatata ggcgttatcg atgttcggga actgacgcag atctttagtt ttgatgtttg
    15481 ctttcgcgta atcaacggag ccagtgccgc tcttcaccgc aaccactttc ccgtcgagat
    15541 ctttcacgct tttcacatcg ttattgttag ctttcaccat cactaacagg ccgcttttgt
    15601 agtagccgtc agagaaatcg atcgcttttt tacgctcgtc ggtgatggta atgcccgcca
    15661 gcgccagatc gacgtttttg gtttgcagtg ccggaatgat cccactgaaa tccatcggct
    15721 tcagttcgta atccagcttc agctctttag cgatggcagc ccacagatca acgtcaaagc
    15781 ccacatattt atcgccctgt ttaaattcaa acggaacgaa ggcggtatcc gtcgcgacaa
    15841 ctaatttttt atccgcggca tgagaagaaa ccgcaaaagc cagggtcagt gcagccagtg
    15901 aaacttttaa tacagacttc atagcatttc cttttttcta tccacgggac gatcccctgc
    15961 gtgacattca catatatgaa aaaatcgtgc cagttttgca actcattgat ttttcgagat
    16021 gcggcaaaaa cgtgatgcac gatttatagg gcaacaccct gaagatgcac cattctgggg
    16081 caccaaactg gtgcgctaaa attgtgcact caacacagtg attatttagc gtaaaacctg
    16141 atgaaaaaac aatctttatg taacgattgt gtgatgatgt ggatacaaaa aatttactta
    16201 atcagctggg gatagcagat ggatgcacta aataagtgcg ttgaggcggg ctgcaaaaca
    16261 aaacggcctc ctgtcaggaa gccgctttta tcgggtacta aaattctgca ccatcagcga
    16321 tggatttatt cgatgttaga ctcgataaac cacaggaatt tatccaggtc gcgagacgcg
    16381 gcggtcagga tatctgcggt gtcgtcatct ttcgcttcgc caatcgcttt gcgtacgtca
    16441 ttagcgacga ttgcgtaacg gtcagccagt tctttcaggt gatcctgaac gttgtggatg
    16501 tccagcgggt aacttttcag cggggttttg ctgttgataa cttgagtggt ccccagagct
    16561 acaccgccca gctgcactgc acgttctgcc atggtatcca gatgatcgat cagtgcagtg
    16621 cggaagccat ccagcatttc atgtacggca atgaagttag cgccgcgcat gttccagtgc
    16681 gcttgtttgg taatcaaaga aagatcaata aactggataa cctggcgatt cagcaactct
    16741 actgttgctt ttttctcgct gtcagagaca tcgttgcggg tataaagcag attggtcgct
    16801 tttgatttaa ctaatttagc ggtactcata atttcatatc ctcttgatgt tatgtcccag
    16861 taattaacga gactaagtat agcaccggct atgtgttccg ctattctggc tgttcctatc
    16921 acactaatag tggtaacaag cgtgaaaaac aaaactaata aagattcaat gagttagata
    16981 tattgataag aacaattctt atttatttgc tcgccaggaa ttttcggaat aaatatgctt
    17041 ttgcataatt cgatatataa agacggtgta gaggaaaagt agcgagaaat tctgcatggt
    17101 tatgcataac catgcagaaa tgtaaattta attaatgtct aattctttta ttttgctctc
    17161 tttgcgtact gtcagcgtag aacccattga agcggcgata attgcgccga gcgccagcag
    17221 ctgtatgggt gtcagtgttt ctccgaggaa aatcatcccg gaaacggcag ccagcgccgg
    17281 ttccatgctc atcagcgtac caaatgtccg tgttggcaaa cgggtgaggg caatcatttc
    17341 cagcgaataa ggcagagcgg tcgagagaat agcgaccgcc agacccaatg gaataaccga
    17401 ccagtgccag agtgcttcac cagcctgaag cgctccaatt ggcacgaaaa ttaacgctgc
    17461 aatcaacgaa ccaattgcca ccgtcgcagg gccatgttcc gctcctgcgc gttgcccact
    17521 taaaatgtaa atagcccaac aagccccggc ccccagtgcc agcgcacagc cggttaaatc
    17581 gacatgggaa acgtcttgcc ccagcggtag caggaaccac agaccaagaa ccgccagcac
    17641 aacccagacg aaatctaccg ggcgacgaga agagaacagc gccaccgcca gtggtccggt
    17701 gaactccagc gccaccgcaa tacccagcgg taccgtctga atagagagat aaaaaagata
    17761 attcatccca cccagcgaaa cgccgtaaaa caacagcggt aaccgttgct ctttggcaaa
    17821 gcgcagtcgc catggcttaa agaacgcgat gaggatcagc gttcctaatg ccaggcgcag
    17881 cgcagtgaca cccggtgcgc ccaccagagg aaaaagtgac ttagctaacg aagctccacc
    17941 ttgaatagac gccatggcaa cgagcaatat gactattggt aaccagaccg gcattttacg
    18001 taatgaacca ggcatccttt ctcccacaaa tatctagact taagtaaagc gtggagtgta
    18061 ctggatatac ccaatgctgg ttgagcattt gttgaaaaaa ttttcccccg ttttgactaa
    18121 aatgcgccag gattgatgga atcattagtc tggtgattag gaataatctg gatgaatgac
    18181 agggaaaaca tgcgtaatac ttacgcagtt ctctgaaaaa gtgatttaaa tttagatgga
    18241 tagcggtgta tggaaacgtt ctgttacatg aaatggcccg ttagacatca caaatcgcga
    18301 agagtttccc attaattttt gatatattta aaacttagga cttatttgaa tcacatttga
    18361 ggtggttatg aaaaaaattg catgtctttc agcactggcc gcagttctgg ctttcaccgc
    18421 aggtacttcc gtagctgcga cttctaccgt aactggcggt tacgcacaga gcgacgctca
    18481 gggccaaatg aacaaaatgg gcggtttcaa cctgaaatac cgctatgaag aagacaacag
    18541 cccgctgggt gtgatcggtt cattcactta caccgagaaa agccgtactg caagctctgg
    18601 tgactacaac aaaaaccagt actacggcat cactgctggt ccggcttacc gcattaacga
    18661 ctgggcaagc atctacggtg tagtgggtgt gggttatggt aaattccaga ccactgaata
    18721 cccgacctac aaacacgaca ccagcgacta cggtttctcc tacggtgctg gtctgcagtt
    18781 caacccgatg gaaaacgttg ctctggactt ctcttacgag cagagccgta ttcgtagcgt
    18841 tgacgtaggc acctggattg ccggtgttgg ttaccgcttc taatcacttt ggtgatataa
    18901 aaaatccgcc tctcggggcg gatttttgtt tttaaggctt cgggtcgaaa atatcggttc
    18961 cgagatggtt gtaatccacc ttctgtaact ggaagttggt gatgtatatc ggcccggctt
    19021 tcttctcaga tataaacggg tatttgatgt taatttcctt cgctttaatt cccgtccact
    19081 gcgagaaaaa gcctaagaag tcattggctg agcggcgggc tttaatcacc cgatgcgctt
    19141 tatcgtcgct ggaaatgacc ataaaaggca cctggaaatt ttgctgatac ttatcatcat
    19201 gggcaaggta ttgcacgtct ttgccgcgct ctttaaaggc cagaccgtgg tcagaaaagt
    19261 aaaccagcga gaagctgctg ccgctgttgc gtaactgatc gtacagcttg cgcagtaaat
    19321 cgtccgtttg cgtcatagta tagagatagc acgacgtttc tttcgattgc acaaaggttt
    19381 cgtatttgcc ttttgtccgg tcgcaggcct gcggatgcga gcccatcaga tgcagaacaa
    19441 tcagctgcgg ttgcgagtgc tcttgcgcca gcacttgagc ggtcatatcc agtaacgctt
    19501 cgtctttcgt gtttttatct gcttcaaaat taccttcttt caggaagtac acttcatctg
    19561 cacgtttggc gatgctggcg atagcagtat cgtattcgcc gatttggccc tggttggaaa
    19621 accaccaggt ctggaagccc gcgcgatttg ccagggtgac aaagttatcc tgaaactgtg
    19681 gtttgccatc gactacgcga ttgagcgtta agccaagcga tttctgcgtg gagccactgg
    19741 cggcaatgta gtcagcaaat atcaaaccgt taacgctgct ggcaaacggg gtattgtccc
    19801 agtgaccgcc aaaggcaccg agggcatcgc gacgcgcgct ttcaccgatc accaccacat
    19861 aggtctgata cttcggctta acggcagtga ccgtccaggt atctttcatt ccggaaagtt
    19921 ttgccatccg ttcttgctct tcaatcacct cgttattatt gacgatgaca tccttagcaa
    19981 aacgaaatac cggatagcca gtatctttca gtttaaatac gccaccccag gccaggtttt
    20041 gcacgggagc aacaaaaaat gtcgccacac tgaataccag acacaggctg tcgaaggtat
    20101 tccagcgcgc tttttcattc tctttttttc gcctgattgc tattacgccg agcgcaaaaa
    20161 taaataagcc gaccaggtag ctgtaccacg gaaaaatcgt caggatttcg gtcgactctt
    20221 ccatattggt ggagtgcaat gccagcaatg tattgaaatt aggcgcgccg taggcctgag
    20281 caaaagggaa ataacaagcc gccaccagcg aactgacacc gaccagaact ttttgtacgc
    20341 gaggcaatgt tcgccatagc aaaagcaaaa tagccgtaaa cgcagcggtg tagagcaaac
    20401 tgaaggggta acctaagccg aggttaatta atagcgactg taaaaagtag aacgcagtcc
    20461 acgggctaaa tacccggcta cgggtaacaa gcgattcttt gagggttaaa ttcatatgcc
    20521 actatcgtaa caaacgccat gtgcttaccc tggcgtcaag ggtcaatacc tgcaagagtg
    20581 cgtgaagagg ggaaaggggt ccgcatccgc gagccgcaat atacgcaggg ctgcaagaag
    20641 atagagcgag ccgcagttca ggtcaactac tgacgaacaa atcttttgcg aaggggattg
    20701 caaatccgac aaaaagtggg gtttttcaga ccaaaacgcg tcgtgcctgc atgaaagcag
    20761 gagtgaagcg gcgtaccgcg acgccgctat ttatcggaag gtttatcttg ctgcggtttg
    20821 ttgttgacca tatcgcacaa catagagagc agcattaacc gtactttaaa gggagaatga
    20881 ctaaacacgc gcatacacct cttgaactca ttcataagac ctcctgactt gctaatcccg
    20941 tcgatccttg agggatgatt gcattacata cagatatagc acaggctata ttatatagct
    21001 attgctaaaa cgttaatttt ttgtaccttc gcaactctgg tttacaatgt gcgcacgaaa
    21061 tgagaacgct atgtatgcgt caccataatg aggaagcaca atgagtcgtc gcgcaggtac
    21121 gccaacagca aaaaaagtga cgcagttagt gaacgaggaa gagcacgttg aagggttccg
    21181 ccaggtcaga gaggcgcatc ggcgcgagct tattgatgat tacgttgagc tgatttctga
    21241 cttgatcagg gaagtggggg aagctcgtca ggtagacatg gctgctcgtt tgggagtttc
    21301 gcaaccgacg gtggctaaaa tgcttaagcg actggcaact atggggctga ttgaaatgat
    21361 cccctggcgt ggcgtgtttt taacggcaga aggagagaag ctggcgcagg aaagccgcga
    21421 gcgacatcag atagtcgaaa atttcttgtt ggtgttgggc gtcagtccgg aaatcgcccg
    21481 tcgcgacgcg gaaggcatgg agcaccatgt tagtgaagag acgctggatg cctttcgttt
    21541 gtttacccag aaacacggtg ccaaatgagc ctgccttttt tacgcacgct gcaaggcgat
    21601 cgtttttttc agttattaat tcttgttggt atcggattaa gttttttcgt gccctttgca
    21661 ccgaaatcct ggcctgctgc tatcgactgg cacaccatca tcaccttaag cggcctgatg
    21721 ctgctgacca aaggtgtgga gttaagcggt tattttgatg tgctggggcg caaaatggtg
    21781 cgccgctttg ctacggagcg tcggctggcg atgtttatgg tgctggcggc ggcgctgctt
    21841 tctacctttc tgaccaacga tgtcacgctg tttattgttg ttccgctgac tatcacgcta
    21901 aaaaggctgt gtgagatccc ggttaatcgg ctgattattt ttgaggcgct ggcagtcaac
    21961 gctggttcgc tactgacgcc aattggcaac ccgcaaaata ttcttatctg gggacgttct
    22021 ggtctttcgt ttgccggatt tattgcccaa atggcaccgc tggctggcgc aatgatgctg
    22081 acgctcctgc tgttgtgctg gtgttgtttc cctggaaagg cactccaata ccatacgggg
    22141 gtgcaaacac cggagtggaa accgcggctg gtgtggagtt gtctggggct gtatatcgtc
    22201 tttctgacgg cgctggagtt caaacaagag ctgtggggac tggtgattgt ggcggcgggc
    22261 tttgcgctgc tggcgcgtcg cgtggtgttg agtgtggact ggacgctgct gctggtgttt
    22321 atggcgatgt ttatcgacgt ccatttactg acccagcttc cagcgttgca aggcgtgttg
    22381 ggtaacgtga gtcatttatc tgaacccgga ttatggttaa cggcaatcgg tttatcgcag
    22441 gtgatcagta acgtgccgag tactatattg ttgctgaact atgtgccgcc gtctttatta
    22501 ctggcatggg cggtaaacgt aggtggcttt gggttattac ccggatcgct ggcaaatttg
    22561 attgcgctac gtatggcgaa cgatcgccgc atctggtggc gtttccatct ctattcaata
    22621 ccgatgctgt tgtgggcggc gctggtggga tatgttttgt tggttatgat cccggcctgg
    22681 taaggtctgg caaataaaaa aggcggatta tgagtccgcc tttgttttac gttttgaaga
    22741 ttaattcaga cgaaccggca tcccggagcg gtttttaatc gcttcatcaa gaacaacctg
    22801 gtcaacatct ggctggccgg tcactgtctg cacgctcttc gtcagggtaa ttggcacaat
    22861 ttcctgacct tcaaattggg cttcggtggt agacagcggg ttatggactt caatgtaacg
    22921 gctgccgtct ggctcggtgg tcgcttttac cggctcatca ataaactgta cgcgggtacc
    22981 gaccagtact ttctcgaaca ggaatttgat gtcttcgtta cgcagacgca cacaaccatg
    23041 acttacacgc agaccgatac cgaagttagc gttggtgcca tggatagcgt acaggcgacc
    23101 gatgtagagt gcatacagcc ccatcgggtt atccggacct gccggaacga cagccggaag
    23161 cggttcgccc gcagcgcggt actctgcgtg cattttggcg gtcggcgtcc aggtcgggcc
    23221 tgctttctta cgctcaactt tggtggtcca gttgataggc gtatctttgc ctaactgacc
    23281 aatgccgatc ggcagcacga taacggtgtt ggtccctttc ggatagtaat acagacgcat
    23341 ctctgcactg ttaatgacga tgccttcatg aacggtatcc ggcaggatca gctgctgcgg
    23401 aatgttcagt acagtaccgc ctttcggcag gaaggtatcc acacccgggt tcgcttccat
    23461 catattggaa agccccatct ggtactccgc ggcaaaatac tccagcggct gagtgttacc
    23521 ttcaggaatg gtgatcacct gattctgacc aaccaggcga ctcccgtcgg ttggcagagg
    23581 ataagttacc gcagaggcgg tactgcaaaa gccgacaaca gcgaaggccg ctgcgaataa
    23641 tgttttcaat ttcatattca tgttaagcga gattttgtgc ctggcaggcc attgggttga
    23701 gaatattaga gtattggaag cgcattataa gttcattcca gctcacagtg aaatcagatg
    23761 tgtacgaaat cacatttttt gcctttggct tgagtgtaga tcttaagcga ggagcaggat
    23821 cttctttcag acttatggca taatgcgcgg tttgtcatat ctcttttcag gatacgcctg
    23881 tgttagtttc cagtaacgtc accatgcagt tcggcagtaa gccgttgttt gaaaacattt
    23941 ccgtcaaatt tggcggcggc aaccgttacg gcctgattgg cgcgaacggt agtggtaaat
    24001 ccacctttat gaagatcctc ggcggcgacc ttgagccgac gctgggtaac gtttccctcg
    24061 atcccaacga gcgcattggt aaactgcgtc aggatcagtt tgcctttgaa gagttcactg
    24121 tgctggatac ggtgatcatg gggcataaag agttgtggga agtgaagcag gagcgcgacc
    24181 gcatctatgc tttgccggaa atgagtgaag aagacggcta taaagtggcc gatctggaag
    24241 ttaaatacgg cgaaatggac ggttactctg cggaagctcg tgccggtgaa ttgttgcttg
    24301 gcgtgggaat tccagtggaa cagcactacg gcccgatgag tgaagttgct cctggctgga
    24361 agctgcgtgt gttgctggcg caggcgctgt ttgctgatcc ggatattctc ctgctcgacg
    24421 aaccgaccaa caacctcgac atcgacacca ttcgctggct ggaacaggtg ctgaacgagc
    24481 gtgacagcac catgatcatc atctcgcacg accgtcactt ccttaacatg gtctgtaccc
    24541 acatggcgga tctggattac ggcgagctgc gcgtttatcc gggtaactac gatgagtaca
    24601 tgacggcggc gacccaggcg cgtgaacgtc tgctggccga taacgccaag aagaaagcgc
    24661 agattgctga gctgcaatct ttcgttagcc gctttagcgc caacgcctcg aaatctcgcc
    24721 aggcaacttc gcgcgcgcgc cagattgata aaatcaaact ggaagaggtg aaagcctcca
    24781 gccgtcagaa cccgttcatc cgttttgaac aggataagaa gctgttccgt aacgcgctgg
    24841 aagtggaagg tctgaccaaa gggtttgata acggtccgct gtttaaaaat ctcaacctgc
    24901 tgctggaagt cggtgaaaaa ctggcggtac tgggtaccaa cggcgtcggt aaatcaacgc
    24961 tgttgaaaac gctggtgggc gatctgcaac cggacagcgg caccgtaaaa tggtctgaaa
    25021 acgcgcgcat tggttactat gctcaggacc acgaatatga gtttgaaaat gatctgaccg
    25081 tgttcgaatg gatgagccag tggaagcagg aaggcgatga cgagcaggcg gtgcgcagta
    25141 ttctcggtcg tttgctgttc agccaggacg acatcaaaaa gcctgctaaa gtgctttccg
    25201 gtggagaaaa agggcggatg ctttttggta agttaatgat gcagaagccg aacattctga
    25261 tcatggacga accgaccaac cacctggata tggaatccat cgagtcactg aacatggcgc
    25321 tggaactgta tcagggcacg ctgatctttg tttcacacga ccgtgagttc gtaagctccc
    25381 tggcgacccg cattctggaa atcaccccgg aacgcgtgat cgactttagc ggtaattacg
    25441 aagattacct gcgtagtaaa gggatcgagt aagttattat ccgtcacccc gatactgtcg
    25501 gggtgacggc ggctgatgac aaacgcaaga ttgcctgatg cgctatgctt atcaggcatt
    25561 aagaaagcgc acttgtcagc aatctgagta acgtcgaatc gattttctta atcccacatc
    25621 aattcacttc aattaactct caaacatcca tgcccaacgc tcgcttaccg tgaatgttga
    25681 ggtcggcaag cgtaaagcgt ccttcccagt ttgtttcata gtcctcgcgc gggaagtcgc
    25741 ccggcgatgc gcctttttcc agtgcggcct tcaccttgtg cgcgtaggca agatttttct
    25801 cgcacatcgg tgccgcagga atgtacatca cgttgcccca accctgttga ttttcaacgg
    25861 gggcaacgga atggatgacg tcgcagtgcc accagacgga gtctccggcc tcgagttttg
    25921 gaatgctggt taacgcctca attaacagtg gatgccattg ctctgatacc ggcaacactc
    25981 ttccgggcgc tacgccgcac agttcatcct ccggcacatc atcaagcagc ggacgtaaca
    26041 gtacgtacgc catggcttca ggaatgggca cgacgtgcag caacccctga ctaggcagca
    26101 tatcagagag cgctgtccag ccctggaatg tccgaaacac ggaacatttg gtggtgttgt
    26161 ccaccgtgta ctcttcaact tccgtacgat gtgccgcatg ccagggatcg tatttcgcca
    26221 gattgccatt aaagacgtta gcgaaaacgt gctgatacgc tggaagcagc cagcgttcca
    26281 gcgccccgga gtcggtatgc gctccaagac ctttggaggt cgttccgggc ggacggcggc
    26341 ggatacggtc agggtagatg acgctcacat ccgggttaaa ccattgcttt ccatcacttt
    26401 caaatgtcca cagacgattg agaaacgact gcgcattcgc catttcttca ctctggcggg
    26461 cctgcatttg cgcctgcgac cagtagatgg ggtaaatctc gggacgtgaa gcgctgagcg
    26521 tcccgaagaa attatcgccg gggcctttgt agacctcgtc aaagcggttg cggtccagat
    26581 agtccagcat cgactgatcc cagcctagcg cttgttcgcg ggggaaatgg ccttttatca
    26641 ccgcacaacc gcgacgttta atctgttcgc gctgctctgc agtaacatga cctgctttga
    26701 tatcggcata agacagcacc ggccagacgg catcgccctg tgctttgagt gcgttgattt
    26761 cagccactcg cgtggcaatg tcatcgctta gctgattaaa gatctgctgg acgtcgccaa
    26821 gctgcgcccg cagcgcgtgc ttcatctgac ggatagctgc tttgtgatcg gcaggcaatg
    26881 tgtcgctggt aaaagtagaa gccataacca cctcgcatta actttcattc gaaactatgc
    26941 tgatttgcat gtgacaatat aagttataaa taagttaatg caagtttaat gatttgatgt
    27001 gatgtacagg atgtaaaaag aagggataac cggagcgcat ggctccggta gagaggtcag
    27061 ctgttaaaag gggatgtgtt atccagtaca gcctgaatca cattcagcgc gccttcatga
    27121 ttattatcat cggtagcgta acgggcgatt tgtttaatgt tttccgcagc attgcccatc
    27181 gcaaaggaat aacgcgccat tttcagcatc tccgcatcgt taccgctgtc gccaatcgct
    27241 accacatttt gcggtgacag atcccagcgt ttcagtaacc gcgaaatacc gtttgcttta
    27301 tgtagaccgg gaataatcag gtcgataaag ccaaaaccac tggtgacggg tttcataatg
    27361 ccatcgagcg ctatgtgcag tttgtcgatc actaacggga tttgttcatc cggcaggttg
    27421 agtgaaaact tgaacagtac gtcgtcaatc tcctgataat cttttacagg tttcaggcga
    27481 tggtagtgtt ttgccatcag tgcgacaaat gcttcggggg cattttcgct gacatatgca
    27541 ctttgcagac cgcaggcgac aaaattgagt tgcttatctt ttagcaactc gccaataaca
    27601 atccgcgatt catgtcgggt cagttcgccg tggaacagtt gcttgccatg ttcgtaaacc
    27661 agtgcgccgt tttccgcgac aaaagagatc tcatccttta gctcaggaaa gaatgaaata
    27721 agctggtaat actgattacc gctggcgaca acgaactcaa tgccgcgctt tttcagttcc
    27781 tgatattgcg ccataaaacg tggttggttg tacgttttga cgtcgttaag aaaagtaccg
    27841 tccatatctg tgacgataac ttttacgctc ataggtgtgc tcccggctcg aaaatgaaac
    27901 cgtaacagtg taataacaat gtgacgcaga gcacaaatta tatttcgaat gaaagtaagg
    27961 atgaaattga tgatgtgaat gatttagccc ggcgacgtct ccgccgggct gaggggatta
    28021 cagcatatgt tcggtacggg cgatgatatc gtcctgagcg tctggagaga gcgcggtgaa
    28081 gaaggcggaa taacccgcga cacgcactac cagatcgcga tactgatcgg gatgtttttt
    28141 cgcctccagc agcgtttcgc gggaaacgat gttgtactga atatgccagc ctttatgcac
    28201 ttcaaagaag gtgcgcagca ggatcatcag tttctgcttg tcagattcgt tctccagtgt
    28261 tgctggattc agtttctggt tgagcaacac cccgccgaga atcgctgccg taggcagttt
    28321 acccaccgag ccaatgaccg cagtagggcc aagatgatca gtaccggaag ccgggcttgc
    28381 gccttctgcc agcggggtgt gggctttacg tccgtctggc gttgccatag tttgcgcgcc
    28441 aaacggtacg ttagcggaga tagacgacgt acccgcgtaa tagttgccgc caaccggacc
    28501 acgaccgtag cgcgggttat ggtactgttt cagttcgtcg atataggtct gataagcgcg
    28561 agccagcagc gtatcgacag tatcatcgtc gttgccgtac ttcggcgcac cgttaatcag
    28621 ccgctgacgc agctgctcgt gagtcaggcc gtcgaagtca ttcgccagtg cggcagccag
    28681 ctgttgctga ctaatcgcgc cctgttcaaa taccagtttc ttcatggccg ccaggctgtt
    28741 gccgaggttg gcaatgccaa cctgcaggcc agaaacccag tcatatttcg cgccgccttg
    28801 cttgatactt ttcgctcgct caatacagtc atccaccagc gccgagcaga gaatatcgtg
    28861 cacgttctct tccagcatgg tgtcgacgac atattcgatt tcgattgatt tacgcgtgta
    28921 gtaacggatt tgcgtatccc acgcgtccat cacttcatcg aagttgttga agttacctgc
    28981 cgacaacgct ttttcttgtg gcaggaacac tttgccgctg gtggcatcac gcccgccttc
    29041 cagcgccgcc agcatcacgc gggcgaagtt gataaagctc atgccggtac agcgatagcc
    29101 ccatttgcca ccgacggcgg tttcgataca accaatcgct gcatagttgt aagcatcctg
    29161 cggttcaata ccgagtttaa taaattccgg gatgacgatt tcgtcgttgt tgaacgccgg
    29221 catcccgaag ccgcaacgga tcacctgtac gcaggcgtcg aggaaatcgt tgctcattcc
    29281 cgcgtggtaa cgcacgctga ggttaggctg agtggaacgc aggcgaccgc aggattcgag
    29341 gatcgcgtaa gagagtggat tcaccgcgtc cattggttga ccatcaacca gattttgtcc
    29401 gccaatggtg acgttctgat acagaggact tcccgcagag gcttttgagt gtgagcctga
    29461 gcggattttg ttcacttcca gcagtttcag ccagcagcta tgcagcaatt cgatggcgtg
    29521 ttcgcgatcc agtgtctggt tgagttcaac gtcgcggcga tagtacgggt agagatactg
    29581 gtccatacga ccaaacgata ctgagtgacc gttagattcg atctgcaaaa tcaactggat
    29641 gaagtaacac agttgcagcg cctgccagaa agtctgcggc ggctggtggg cgataagatc
    29701 gcagttttct gccatcgcca gcagttcatc gcgacggctt tcgcgggttt cggtcgcggc
    29761 cattgcacgc gccagggcag cgaaacgttc aatgtgttca ctgactgcca ccagcacgat
    29821 atcaatcgct ttcaggaact gttcgccgtg cagatcttcc agtaccgtca gattgatgcg
    29881 cgagcgacgt tccgctactt tctcgcgcag accatcaagc ctttttccag cagcagcggg
    29941 aaattcaccg ccaggtgcgc atcgccggag gtcatattgc cttccgcttt aatgattccg
    30001 gtcgccagca gacctttttg ctcatcggta aacatgccgt agcagcgatc ctgtaccgtc
    30061 tgaccgcgcc accacgggca cacttcatgc agaacgcgtt tgttctcttc gctcaccgca
    30121 aagccagcac cgggacgatc tgccagatca tcaatctctt tttcgatcca cgagacagta
    30181 tattccggga agatcggcgc ggcgcgaact tcgcttgcct ggttgccgat gatcaactca
    30241 tcgtgcttga tccagatagt gcgattcgcc aggtgatgtg ccagcgccag cgcgcgacgt
    30301 acagggatcg gcttatcgag atgttgttga tacatctcgg tatagtgctg cgcgcgctcg
    30361 gtacaaaccg gcggtttcac aatatgcacc agcgcatttt tgtgcgcttt aatgcggtcg
    30421 ctgagcgtgt ccagtttcag tgtggtcatg gttgttatcc tcgtaaggtc gcggttaacc
    30481 ctttctggca ggcatactgc tgggcaaagt cgagcagttc tggcgcatca agcggttttt
    30541 ccggggcgtc atagggcaga ttaagtaagt gatatttgtt gatgcccagc gtgtggtagg
    30601 gcaggaaatg aatttcgcca acgtgcagct cgtcggcggc aaaatcggta atggctttca
    30661 cagaggtttc gtcggcatta aagccttgaa tcagcggcac gcggataatg atttttttgc
    30721 ccgctgcggc gagttttttc aggttatcca gcaccctggc ggcgttaccg tcggtccact
    30781 gtttaaacgg cgcgtcggca acgtgtttta aatcggcaag aaacagatcg atatagggca
    30841 gagaaggggc gatatatttc cacggcacat gcaggcaggt ttctaccgca gtatgaatgc
    30901 cggcctcgtg gctggcttgc agtagcgccg tcgccatttc cggctgcatg aatggttcac
    30961 cgccagaaag tgttaagccg ccgccgctgc gatcgtaaaa cggtttatcg cgcagaacgg
    31021 tcaccatgat ctcctcaacg cttttcactt ccccacacac ggttaacgac tgtgtcgggc
    31081 agcagtcggt taacgccgtc agatgctccg gggttaactt ttcccgatga ataagcaaac
    31141 cattcagcgc gcgctcaatc acttccggcg cggtcttagc gcacagctca cagccttcca
    31201 ggcacagacg tgggtcatac agcagatcct gcgtgcgggc gcggctttcc gggttctgac
    31261 accagcggca gcccagcgaa cagcctttaa gaaatacgac cgtgcggata ccggggccat
    31321 catgggtcga gtagcgctga atattgaaaa tcatagttgc ctctctgttt cgttcaaata
    31381 ttaaaatact ttcgaatgaa agttagattg atgtgcgtca actgttcaga gagttttccc
    31441 gtgatagtct acattcagac aaaaagtaca ttttgaggat ggttatggaa ctgtatctgg
    31501 atacttcaga cgttgttgcg gtgaaggcgc tgtcacgtat ttttccgctg gcgggtgtga
    31561 ccactaaccc aagcattatc gccgcgggta aaaaaccgct ggaggttgtg cttccggaac
    31621 ttcatgaagc gatgggcggt caggggcgtc tgtttgccca ggtaatggct accactgctg
    31681 aagggatggt gaatgacgca cgtaaattgc gttctattat tgcggatatc gtggtgaaag
    31741 ttccggtgac caccgagggg ctggcagcta ttaagatgtt aaaagcggaa gggattccga
    31801 cgctgggaac cgcggtatat ggcgcagcac aagggctgct gtcggcgctg gcaggtgcgg
    31861 aatatgttgc gccttacgtt aatcgtattg atgctcaggg cggtagcggt attcagactg
    31921 tgaccgactt acaccagtta ttgaaaatgc atgcgccgcg ggcgaaagtg ttagccgcga
    31981 gtttcaaaac cccgcgtcag gcgctggact gcttactggc aggatgtgaa tcaattactc
    32041 tgccactgga tgtggcacaa cagatgatta gctatccggc ggttgatgcc gctgtggcga
    32101 agtttgagca ggactggcag ggagcgtttg gcagaacgtc gatttaaccg gacgttctgc
    32161 atcctcataa attgctgatg acgtggcgga gtgcaccgat gcctgccgcg cttggaaatc
    32221 tgtgcccgaa ccgtaggcca gataaggcgt tcacgccgca tctggcgtct ggagcaaatg
    32281 cctgatgcga cgcttgtcgc gtcttatcag gcctggaggt ggcaattact gcccgcacac
    32341 ctcacacccc ggattgcgca tcagtttcat ttcgcgaaac tgacaggtca tcgcatcgta
    32401 catgacgatt ttcccgctgg ccggtttgcc ataacctgcc agcagtttga tcgcttccat
    32461 tgcctgcaac gaaccaatca cgccgatcaa cggtgccatc acgcctgctt ctacgcaggt
    32521 taacgcgttc tcgctaaaca aacggctaag gcagcgatag cacggttcgc cgtcctgata
    32581 agtgaagacc gtgatttgcc cttccatacg aattgccgcg ccggaaacca gcggcacctt
    32641 cgcggcaaaa cagcctgcgt tcagttgatt acgtaccgca acgttatccg tacagtcgag
    32701 cacaagattg tgttcagcaa tcaatgctgc aagttctgcg tcatccagca gtgcattgac
    32761 tggcgtaatc gcgatatgtg ggttgatccg cgtcagagcg tcacgggcgg attccacctt
    32821 cggttgcccg accgtagcat cactgtgcag cgtctggcgt tgcagattcg agagcgaaac
    32881 cgagtcgaag tcgagcagcg tcaggttgcc gacaccggcg cttgccagat actgcgaagc
    32941 tgcgcagccg agtccaccga ggccgacgac cagcacacga gaatctttca gcgcctcctg
    33001 accgtcaaaa tcaaaaccgc gcagaatgat ttgccggttg tagcgcagca tctcttgatc
    33061 gctgagttcc gccattacag gcctccgaac aacgcgttaa acggttctac ttccacccat
    33121 tcgcccactt ccacattgcc gcgatcgcgt tccagcacga taaagcagtt gccgaggcta
    33181 aaggagctga atatatgtga accctgatgt ccggtggtcg tcacttccag ttcgccatcg
    33241 gcgttgcggt gcagcacgcc gcgctggaaa tcaagacgtc ctggcgtttt tttcaggcgg
    33301 gacgctgtgc gtacgcgctg gcgcgcgggc aggccgctgg cggtgttgcc gcttagtttt
    33361 gccagcaaag gctgtaccag ttgatagaag gtcagcgtcg ctgaaaccgg gttgcccggc
    33421 aggccgcaga accagctatt gctgagttta ccgaacgcga acggtttacc tggtttaatc
    33481 gccagcttcc agaaggcgat ctcccccagc tcttcaagaa tcgttttggt gtaatccgct
    33541 tcacccactg aaacaccgcc ggaactgatc accacatccg cctggctgtc ggcttcaata
    33601 aatgcggcgc gcagggcatg tggatcgtcg cggataatcc ctaagttgat tacctcgcaa
    33661 cccagctgtt ctaacatcag gtgtacggcg agacggttgg tatcgtagat ttggccgtcg
    33721 cccagcggct ggcctggcaa ctggagttca tcaccggtag aaaaaagcgc tacacgcact
    33781 ttacgaatca ccggaacttc ggcaatcccc agtgaagcaa ttactggcag ctctgcggta
    33841 gtcaggcgag tccccgccgg gaaaacaacc gcacctgcag agatatcttc accgcgacgg
    33901 cgaatatttt gcccgctacg cacttcagca gtaaaacgca cgccattgtc cgtttgttca
    33961 gtctgctcct gcatcaccac cgcttcgcag ccttccggca ccggcgcacc ggtcataata
    34021 cgaatgcagg tacccgcagg ccattcacca tggtacggct gaccggcaaa ggatttaccg
    34081 gcaacgggca gcggttgtcc ggaggtgata tcggccaaac gtaccgcgta accatccatt
    34141 gcggagttat caaaccctgg gacatccagc ggcgaaacga catcgctcgc cagaatacga
    34201 ccaaaacact gaaccagtgg cagcgtttcc tgggcggtca gtggggtgac gcgagaaagc
    34261 atctcattaa gcgcggtgtc gagcgacatc aatccggtgg taaattccat gaaaacactc
    34321 ctgcggaggc aaaatcgaat ttgcctatta tgtcagaaaa acgccacaga ctgtatgcca
    34381 cctcgggcgt agcgctgggt cctgccttta catgccatat ccatctttct atattcaaaa
    34441 attgaatgag taattcataa aaattctgat atttatagca aaagtggcga gccaccctta
    34501 atggacgaat actatgggca aagcagtcat tgcaattcat ggtggcgcag gtgcaattag
    34561 ccgcgcgcag atgagtctgc aacaggaatt acgctacatc gaggcgttgt ctgccattgt
    34621 tgaaaccggg cagaaaatgc tggaagcggg cgaaagtgcg ctggatgtgg tgacggaagc
    34681 ggtgcgtctg ctggaagagt gtccactgtt taacgccgga attggcgccg tctttacgcg
    34741 tgatgaaacg catgaactgg acgcctgtgt gatggatggt aacaccctga aagccggtgc
    34801 ggtggcgggc gttagtcatc tgcgtaatcc ggttcttgcc gcccggctgg tgatggagta
    34861 aagcccgcat gtgatgatga ttggcgaagg ggcagaaaat tttgcgtttg ctcatggcat
    34921 ggagcgcgtc tcaccggaga ttttctccac gcctttgcgt tatgaacaac taatggcagc
    34981 gcgcgaggaa ggggcaacag tcctcgacca tagcggtgcg ccactggatg aaaaacagaa
    35041 aatgggcacc gtgggggccg tggcgttgga tttagacggc aatctggcgg cagccacgtc
    35101 cacgggcgga atgaccaata aattacccgg acgagttggc gatagcccct tagtgggtgc
    35161 cggatgctac gccaataacg ccagtgtggc ggtttcttgt accggcacgg gcgaagtctt
    35221 catccgcgcg ctggcggcat atgacatcgc cgcgttaatg gattacggcg gattaagtct
    35281 cgcggaagcc tgcgagcggg tagtaatgga aaaactccct gcgcttggcg gtagcggtgg
    35341 cttaatcgct atcgaccatg aagggaatgt cgcgctaccg tttaacaccg aaggaatgta
    35401 tcgcgcctgg ggctacgcag gcgatacgcc aaccaccggt atctaccgtg aaaaagggga
    35461 caccgttgcc acacagtgat gaacttgatg ccggtaatgt gctggcggtt gaaaatctta
    35521 atattgcctt tatgcaggac cagcagaaaa tagctgcggt ccgcaatctc tcttttagtc
    35581 tgcaacgcgg tgagacgctg gcaattgttg gcgaatccgg ctccggtaag tcagtgactg
    35641 cgctggcatt gatgcgtctg ttggaacagg cgggcggttt agtgcagtgc gataaaatgc
    35701 tgttgcggcg gcgcagtcgt gatgtgattg aacttagcga gcagagcgct gcacaaatgc
    35761 gccatgtgcg cggtgcggat atggcgatga tatttcagga gccgatgaca tcgctgaacc
    35821 cagtatttac tgtgggtgaa cagattgccg aatcaattcg tctgcatcag aacgccagtc
    35881 gtgaagaagc gatggtcgag gcgaagcgga tgctggatca ggtacgcatt ccggaggcac
    35941 aaaccattct ttcacgttat ccgcatcaac tctctggcgg gatgcgccag cgagtgatga
    36001 ttgcgatggc gctgtcatgc cgcccggcag tgctgatagc cgatgagcca accaccgcgc
    36061 tggatgtcac tattcaggcg cagatcctgc aattaatcaa agtattgcaa aaagagatgt
    36121 cgatgggcgt tatctttatc actcacgata tgggcgtggt ggcagagatt gccgatcggg
    36181 tactggtgat gtatcagggc gaggcggtgg aaacgggtac cgtcgaacag atttttcatg
    36241 caccgcaaca tccctacacc cgtgcgctgt tagctgctgt tccgcaactt ggtgcgatga
    36301 aagggttaga ttatccccga cgtttcccat tgatatcgct tgaacatcca gcgaaacagg
    36361 ccccccccat cgagcagaaa acggtggtgg atggcgaacc tgttttacgg gtgcgtaatc
    36421 tggtcacccg ttttcctttg cgcagcggtt tgttgaatcg cgtaacgcgg gaagtgcatg
    36481 ccgttgagaa agtcagtttt gatctctggc ctggcgaaac gctatcgctg gtgggcgagt
    36541 ctggcagcgg taaatccact accgggcggg cgttgctgcg cctggtcgaa tcgcagggcg
    36601 gcgaaattat ctttaacggt cagcgaatcg ataccttgtc acccggcaaa cttcaggcat
    36661 tgcgccgcga tattcagttt atttttcagg acccttacgc ttcgctggac ccacgtcaga
    36721 ccatcggtga ttcgattatc gaaccgctgc gcgtacacgg tttattgcca ggtaaagaag
    36781 cggttgcacg tgttgcgtgg ttgctggagc gcgtgggcct gttacctgaa catgcctggc
    36841 gttacccgca tgagttttcc ggcggtcagc gccagcgcat ctgcattgct cgcgcgttgg
    36901 cattgaatcc aaaagtgatc attgccgacg aagccgtttc ggcgctggat gtttcaattc
    36961 gcgggcagat tatcaacttg ttgctcgatc tccagcgtga tttcggcatt gcgtatctgt
    37021 ttatctccca cgatatggct gtggtagagc ggattagtca tcgtgtggcg gtgatgtatc
    37081 tcgggcaaat tgttgaaatt ggtccacggc gcgcggtctt cgaaaacccg cagcatcctt
    37141 atacgcgtaa attactggcg gcagttccgg tcgctgaacc gtcccgacaa cgaccgcagc
    37201 gtgtactgct gtcggacgat cttcccagca atattcatct gcgtggcgaa gaggtggcag
    37261 ccgtctcgtt gcaatgcgtc gggccggggc attacgtcgc acaaccacaa tcagaatacg
    37321 cattcatgcg tagataacat tcaggcggag aataaaatgg caagagctgt acaccgtagt
    37381 gggttagtgg cgctgggcat tgcgacagcg ttgatggcat cttgtgcatt cgctgccaaa
    37441 gatgtggtgg tggcggtagg atcgaatttc accacgctcg atccgtatga cgcgaatgac
    37501 acgttatctc aggccgtagc gaaatcgttt taccaggggc tgttcggtct ggataaagag
    37561 atgaaactga aaaacgtgct ggcggagagt tataccgttt ccgatgacgg cattacttac
    37621 accgtgaaat tgcgggaagg aattaaattc caggatggca ccgatttcaa cgcagcggcg
    37681 gtaaaagcga atctggaccg ggccagcgat ccggcgaatc atcttaaacg ctataacctg
    37741 tataagaata ttgctaaaac ggaagcgatc gatccgacaa cggtaaagat taccctcaaa
    37801 cagccgttct cagcgtttat taatattctt gcccatccgg cgaccgcgat gatttcaccg
    37861 gcagcgctgg aaaaatatgg caaggagatt ggttttcatc cggtgggaac cggaccgtat
    37921 gaactggata cctggaatca gaccgatttt gtgaaggtga aaaaattcgc gggttactgg
    37981 cagccaggat tgcccaaact ggacagcata acctggcgtc cggtggcgga taacaacacc
    38041 cgcgcggcaa tgctgcaaac cggtgaagcg cagtttgctt tccccattcc ttacgagcag
    38101 gccgcactgc tggagaaaaa caaaaatatc gagttgatgg ccagtccgtc aattatgcag
    38161 cgttatatca gtatgaacgt gacgcaaaag ccattcgata acccgaaggt ccgtgaggcg
    38221 ctgaattacg ccattaaccg tccggcgctg gtgaaagtgg ccttcgcggg ctatgcaacg
    38281 ccagctactg gtgtggtacc gccgagtatc gcctacgcgc aaagttataa accgtggcct
    38341 tacgatccag tgaaagcgcg cgaattactg aaagaggcgg gatatcccaa cggtttcagt
    38401 accacgctgt ggtcgtcaca taaccacagc accgcgcaga aagtgctgca atttacccag
    38461 cagcagttag cgcaggtcgg gattaaagcc caggtgactg cgatggatgc cggacagcgg
    38521 gcggcagaag ttgaaggtaa agggcaaaaa gagagcggcg tgcggatgtt ctacactggc
    38581 tggtcggctt caaccggcga agcggactgg gcactatcgc cgttgtttgc ctcgcaaaac
    38641 tggccaccga cgctgtttaa taccgcgttt tacagcaata aacaggtgga tgacttcctg
    38701 gctcaggcac tgaaaactaa tgatccggcg gaaaagaccc gcttatataa ggcggcgcag
    38761 gatatcatct ggcaagagtc gccgtggatc ccgctggtgg tagaaaaact ggtgtcggca
    38821 cacagtaaaa acctgaccgg tttttggatc atgccagata ccggcttcag ctttgaagac
    38881 gcggatttgc aataagcaac gcagggagtg gaatgcttaa ttacgttatc aaacgattac
    38941 tggggttgat tccgacgctg tttatcgtct cggtgctggt gtttttattt gtgcatatgc
    39001 tgcccggcga tccggcgcga ttgattgccg ggcccgaagc tgatgcgcag gttatagaac
    39061 tggtgcgtca gcagctgggg ttggatcagc cgctgtatca ccagttctgg cactatatca
    39121 gcaatgctgt gcagggggat tttggcctgt cgatggtgtc acgtcgtccg gttgccgatg
    39181 agattgccag gcgctttatg ccaacgctgt ggctgaccat aaccagtatg gtctgggcgg
    39241 tgatatttgg tatggcggcg ggaattatcg ccgccgtctg gcgtaaccgt tggccggatc
    39301 gattgagtat gaccattgcg gtgtcgggga tctcgtttcc ggcatttgcg ctggggatgc
    39361 ttttaattca ggtattctcc gttgaactgg gctggctgcc taccgtggga gcagacagtt
    39421 ggcagcacta cattttaccc tccctgacgc tcggcgcggc agtggccgcc gtgatggcgc
    39481 gctttacccg cgcgtcgttt gtcgacgttt taagcgaaga ttatatgcgt accgcgaggg
    39541 cgaaaggggt gagcgaaacc tgggttgtcc tcaaacacgg gctacgcaac gcgatgatcc
    39601 cggtagtgac catgatgggc ttacagtttg gctttttgct cggtggttcc atcgtggtgg
    39661 agaaagtttt caactggccc ggacttggac gcttactcgt tgactccgta gaaatgcgcg
    39721 attacccggt gattcaggcg gaaattctgc ttttctcgct ggaatttatt cttatcaact
    39781 tagtggtgga tgtgctttac gccgccatta acccggctat caggtacaag taaggatgcg
    39841 actatttaac tggcgacgtc aggcggtgtt aaacgccatg ccactggtca aacctgacca
    39901 ggtacgtaca ccgtggcatg aattctggcg acgatttcgc cgtcagcata tggcgatgac
    39961 cgccgcatta ttcgttattt tattgattgt ggtggccatt tttgcacgct ggatcgctcc
    40021 ctatgacgcc gaaaattatt ttgattatga caatctgaat aacggacctt ctttgcagca
    40081 ctggtttggc gtcgattcac tggggcgcga cattttcagc cgtgtcctgg ttggtgcgca
    40141 aatctcgctg gcggcgggcg tgtttgccgt gtttatcggt gcggcgatag ggacgttgct
    40201 gggcttgttg gctggatatt atgaaggctg gtgggatcgg ctgatcatgc gcatttgcga
    40261 tgtgctgttt gccttcccgg gtattttact ggcgatcgct gttgttgcgg tgttgggaag
    40321 cggcattgct aacgtgatta ttgcagtcgc cattttttcc atccctgcgt ttgcccgcct
    40381 ggtgcgcggc aacacgctgg tgttgaaaca gcaaaccttt attgagtcag cacgcagtat
    40441 tggtgccagc gatatgacca ttttgttgcg tcatatcctg cctgggaccg tctcttctat
    40501 cgtggtgttt ttcaccatgc gcattggtac ctcgattatc tctgccgcca gcctgtcatt
    40561 tctcggcctc ggtgcgcagc cgccgacacc agagtgggga gcaatgctca atgaggctcg
    40621 agcggatatg gttatcgcgc cgcatgtcgc tgtttttccg gccctggcta tttttctgac
    40681 tgtactggcg ttcaatttgt tgggcgatgg tttacgcgat gcgctggatc cgaaaattaa
    40741 aggatagtta cgtttgaata ttgcttgaaa gagtaatcac ctcacaggaa attattgccc
    40801 taagcaagtg ttgtaacttt ctgctgattt tgtagaatcg ggtaatttgg ttaaatagcc
    40861 gcagcaaggg actgtttttg cagcggcaca gcgttcagac agttattttg ttaaatgtat
    40921 taacatgctg agtttatacg aaaagataaa gataaggctg ataattttat ttttattggc
    40981 agcactgtca tttattggcc tttttttcat cattaactat caactggtat cggaacgcgc
    41041 ggtaaaacgt gccgatagcc gctttgaact tattcagaaa aacgttggct atttctttaa
    41101 agatattgaa cgttcggccc tgacattaaa ggactcactg tatttattaa aaaatacaga
    41161 ggagattcaa cgcgctgtga tcctgaaaat ggaaatgatg ccatttt
//
LOCUS       DAZKIF010000026        40107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZKIF010000026 DAZKIF010000000
VERSION     DAZKIF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.026"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHOTW_001450"
     CDS             complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHOTW_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP9843585.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2095..2961
                     /gene="yghU"
                     /locus_tag="ACHOTW_001451"
     CDS             2095..2961
                     /gene="yghU"
                     /locus_tag="ACHOTW_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP9843586.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHOTW_001452"
     CDS             complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHOTW_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP9843587.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHOTW_001453"
     CDS             complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHOTW_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9843588.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHOTW_001454"
     CDS             complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHOTW_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP9843589.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHOTW_001455"
     CDS             complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHOTW_001455"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP9843590.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHOTW_001456"
     CDS             complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHOTW_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP9843591.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHOTW_001457"
     CDS             complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHOTW_001457"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP9843592.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQHLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHOTW_001458"
     CDS             complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHOTW_001458"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP9843593.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHOTW_001459"
     CDS             complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHOTW_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP9843594.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHOTW_001460"
     CDS             complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHOTW_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP9843595.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHOTW_001461"
     CDS             complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHOTW_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP9843596.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11268..12308
                     /gene="gpr"
                     /locus_tag="ACHOTW_001462"
     CDS             11268..12308
                     /gene="gpr"
                     /locus_tag="ACHOTW_001462"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP9843597.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHOTW_001463"
     CDS             complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHOTW_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP9843598.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13033..13917
                     /gene="yghA"
                     /locus_tag="ACHOTW_001464"
     CDS             13033..13917
                     /gene="yghA"
                     /locus_tag="ACHOTW_001464"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9843599.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14189..14614)
                     /gene="exbD"
                     /locus_tag="ACHOTW_001465"
     CDS             complement(14189..14614)
                     /gene="exbD"
                     /locus_tag="ACHOTW_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP9843600.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14621..15355)
                     /gene="exbB"
                     /locus_tag="ACHOTW_001466"
     CDS             complement(14621..15355)
                     /gene="exbB"
                     /locus_tag="ACHOTW_001466"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9843601.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15607..16794
                     /gene="metC"
                     /locus_tag="ACHOTW_001467"
     CDS             15607..16794
                     /gene="metC"
                     /locus_tag="ACHOTW_001467"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP9843602.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16934..17593
                     /gene="yghB"
                     /locus_tag="ACHOTW_001468"
     CDS             16934..17593
                     /gene="yghB"
                     /locus_tag="ACHOTW_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP9843603.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17633..18532)
                     /gene="yqhC"
                     /locus_tag="ACHOTW_001469"
     CDS             complement(17633..18532)
                     /gene="yqhC"
                     /locus_tag="ACHOTW_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP9843604.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18726..19889
                     /gene="yqhD"
                     /locus_tag="ACHOTW_001470"
     CDS             18726..19889
                     /gene="yqhD"
                     /locus_tag="ACHOTW_001470"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9843605.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19994..20821
                     /gene="dkgA"
                     /locus_tag="ACHOTW_001471"
     CDS             19994..20821
                     /gene="dkgA"
                     /locus_tag="ACHOTW_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP9843606.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21021..21943
                     /gene="yqhG"
                     /locus_tag="ACHOTW_001472"
                     /pseudo
     CDS             21021..21943
                     /gene="yqhG"
                     /locus_tag="ACHOTW_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            21992..22249
                     /gene="yqhH"
                     /locus_tag="ACHOTW_001473"
     CDS             21992..22249
                     /gene="yqhH"
                     /locus_tag="ACHOTW_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP9843607.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22292..24511)
                     /gene="ygiQ"
                     /locus_tag="ACHOTW_001474"
     CDS             complement(22292..24511)
                     /gene="ygiQ"
                     /locus_tag="ACHOTW_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP9843608.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24622..26034)
                     /gene="ftsP"
                     /locus_tag="ACHOTW_001475"
     CDS             complement(24622..26034)
                     /gene="ftsP"
                     /locus_tag="ACHOTW_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP9843609.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26109..26846)
                     /gene="plsC"
                     /locus_tag="ACHOTW_001476"
                     /gene_synonym="parF"
     CDS             complement(26109..26846)
                     /gene="plsC"
                     /locus_tag="ACHOTW_001476"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP9843610.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27080..29338)
                     /gene="parC"
                     /locus_tag="ACHOTW_001477"
     CDS             complement(27080..29338)
                     /gene="parC"
                     /locus_tag="ACHOTW_001477"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9843611.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29476..31083)
                     /gene="ygiS"
                     /locus_tag="ACHOTW_001478"
     CDS             complement(29476..31083)
                     /gene="ygiS"
                     /locus_tag="ACHOTW_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9843612.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31192..31674)
                     /gene="ygiV"
                     /locus_tag="ACHOTW_001479"
     CDS             complement(31192..31674)
                     /gene="ygiV"
                     /locus_tag="ACHOTW_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9843613.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(31727..32119)
                     /gene="ygiW"
                     /locus_tag="ACHOTW_001480"
     CDS             complement(31727..32119)
                     /gene="ygiW"
                     /locus_tag="ACHOTW_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP9843614.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32271..32930
                     /gene="qseB"
                     /locus_tag="ACHOTW_001481"
     CDS             32271..32930
                     /gene="qseB"
                     /locus_tag="ACHOTW_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP9843615.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32927..34276
                     /gene="qseC"
                     /locus_tag="ACHOTW_001482"
     CDS             32927..34276
                     /gene="qseC"
                     /locus_tag="ACHOTW_001482"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP9843616.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            34386..34967
                     /gene="mdaB"
                     /locus_tag="ACHOTW_001483"
     CDS             34386..34967
                     /gene="mdaB"
                     /locus_tag="ACHOTW_001483"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP9843617.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34998..35312
                     /gene="ygiN"
                     /locus_tag="ACHOTW_001484"
     CDS             34998..35312
                     /gene="ygiN"
                     /locus_tag="ACHOTW_001484"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP9843618.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(35357..36244)
                     /locus_tag="ACHOTW_001485"
     CDS             complement(35357..36244)
                     /locus_tag="ACHOTW_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9843619.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36241..37188)
                     /locus_tag="ACHOTW_001486"
     CDS             complement(36241..37188)
                     /locus_tag="ACHOTW_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9843620.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(37199..38236)
                     /locus_tag="ACHOTW_001487"
     CDS             complement(37199..38236)
                     /locus_tag="ACHOTW_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9843621.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(38245..39228)
                     /locus_tag="ACHOTW_001488"
     CDS             complement(38245..39228)
                     /locus_tag="ACHOTW_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9843622.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39225..40034)
                     /locus_tag="ACHOTW_001489"
     CDS             complement(39225..40034)
                     /locus_tag="ACHOTW_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9843623.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
BASE COUNT         9991 a        10785 c        10063 g         9268 t
ORIGIN      
        1 ataatcagta cgggattgct acaacatgta ttactctttc accacaatta acggttcaat
       61 atcactctct tttttaataa ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt
      121 gccgccaacg gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca
      181 cagttgctga tagatgtttt tctgctcggc aaatttacca ctggttttgt ccagcacctc
      241 ttcatgatgg ctgacgaggt cgatgttgct gccacagcga ccggcgatcg gtttcactgc
      301 gtaacctgtt ttcaccaatt catcattaac agtgaaatcg gtatccagca ggtaacgatg
      361 gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca
      421 cagcggctca aagaccagca cttccgggcg cagcaatacg tcgataagac gcacttcatt
      481 ttgcggatga ccggtacgga tcggcaccgc agcaaactca cggtcgctaa cttcacgaat
      541 ctgatcaaac gcggtttccc acgcccaggt tttccacacg cagttaacca gtcgcccttc
      601 cccatcaatc agttgcccgg cagcatccca gcccagttca tccagcccgc gcaagatacg
      661 cgtttcaaag cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc
      721 gatatctttg tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc
      781 agccagttcg ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc
      841 cgcccaacgt tcgaggatca agcccgcttc ggtatgacag gaggcggagt cggcgttgta
      901 ctcgtaaacc ttcagaccac gctcatccat gcagaaatcc atacgaccgg tgatcatatg
      961 gtgacggcga cgctgccagg agagacgcaa acgtggccag aggattttcg gaatgtcgaa
     1021 cagcgccagc aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg
     1081 cagctcgttg gtggctttaa tcagctcctg ctcggcgctc tcggtaatgg tgtagtaatg
     1141 atacggatcc tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gcagcggatc
     1201 tttttcatcc agccatttac cgtcaaactg gcctttgttt tccagacgcg ctccgctgat
     1261 tttcagcagc tcgcctgcaa tttccggctg cggtaagctg tattcagtat cttccgtctg
     1321 gatcatccag cccagaatgg tggtgtcatc aaaagtgtct ttaagggtat agcagccgtt
     1381 ttcgaccacc atctccagtt cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat
     1441 cacgttctgt tccgcaatac ggactttgtt gccatgtaat tgggtaatga tggcgacatg
     1501 gccagtgtct ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc
     1561 acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttcacg
     1621 caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtaaaga ccacaccgta
     1681 gttcagaaag agaaaacggc gagcaaattc aacgcattgc catttgtggc ccatatattc
     1741 gtcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact
     1801 gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg
     1861 ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca
     1921 gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag
     1981 actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta
     2041 cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca
     2101 gacaatactt atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc
     2161 gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc ggtgggcaaa
     2221 catccattac aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt
     2281 gaggagctgc tcgcgctggg cgttactggc gcagagtacg atgcctggct gatacgcata
     2341 ggcgatggtg atcaattctc cagcggcttt gtcgaagtga acccaaattc gaagatcccg
     2401 gcgctgcgcg atcataccca taatccgccg atccgcgtgt ttgagtcagg atcgattctg
     2461 ctttatctgg cggagaaatt tggctacttc ctgccgcagg atctggcaaa acggactgaa
     2521 acgatgaact ggctgttctg gttacagggc gcggcaccgt tcctcggcgg tggttttggt
     2581 cacttttacc attacgcgcc ggtgaaaatt gagtacgcca tcaatcgctt taccatggaa
     2641 gccaagcgcc tgctcgacgt gctggataag caactggcgc agcataagtt tgttgcgggc
     2701 gatgagtaca ccattgcgga tatggcgatt tggccgtggt ttggcaacgt ggtgctcggc
     2761 ggcgtgtatg atgccgcaga gtttctggat gcgggaagtt ataaacatgt gcaacgctgg
     2821 gcgaaagaag tgggcgaacg tccggcagtg aagcgtgggc gtattgttaa ccgcaccaac
     2881 gggccgctga acgagcagtt gcatgagcgc catgacgcca gtgattttga gacgaatacg
     2941 gaagataagc gtcaggggta aggattggtg ttcgtcgcag caagccatcc aggccggata
     3001 aggcgttcac gccgcatccg gcaatcgagc acaatgcctg atgcgacgct gccgcgtctt
     3061 atcagaccta cgaaagctaa tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt
     3121 gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt
     3181 accagcaccc actggcccag cagatcggca gggttacctt cacaaatcag ggcgatattc
     3241 acgtcgcgct tgataccaca tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca
     3301 gccagcacct ggcctggaac gccaatacac attgttaact ccggttattc aacttcaata
     3361 cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg acagagcgga
     3421 cactgcgcat cgtgctgatg aacctccacc acctggctgc aatcccagca ccaggcctgg
     3481 gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt
     3541 tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcgccaat ttccagccac
     3601 acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gctggataat ttcaacggcg
     3661 ctctggcaaa gcgacaactc atgcattttc gccactcctg cgaccaaaca gcagggcgcg
     3721 acgccctgcg tgtggtacat ccggattcgt gactggcagc gaaaggatca ttcgtgcgca
     3781 gtcatccgtc aggcgttggc cctcttcaat cgacatactg tgcgaaagcg gtgacatcag
     3841 cgaacaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg tcatggtgcc
     3901 atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga gttgatccgg
     3961 gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc
     4021 ttcgaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa aagagagagc
     4081 gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt
     4141 ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggatggct tccacgccag
     4201 attcacgcag cgcagccaga acctgctcaa gcgcaggttc aatcattgct tcaaccgtcg
     4261 gcgttaagcc gatgtgtggc tccagcgatt ccggaattac gccgaccagg gtcagctttt
     4321 tcggaaactc gccggtgaag cgcagggccg acaagacgtc ggccaggcca agctgatgcg
     4381 gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc atcatcgttc
     4441 ccggcgcgct cttcttcgac acaatggcat ccgcaataat cagatgatcg cgatttgcca
     4501 tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca taatccggca
     4561 gaatgtatcg ttgctctaac gcttcgacaa tccgcacacc gatggcttca tcggtcagca
     4621 aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg aaaccacttc
     4681 gttgccgtca gcatccacta cgtgtaccgc acaggccatg cacgggtcga aggagtgaat
     4741 ggtacgcacc acttccagcg gtttattcgg atcagcaacc ggtgtaccca ccagcgactg
     4801 ctcgtaagga ccgacgtcat cattgaagtt acgcgggcca gagttccagg ttgatggaac
     4861 aactgcctgg tagttgctga tgataccgtc tttgatcacc atccagtgag agagcatacc
     4921 gcgcggcgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa tgttcggctt
     4981 cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt attggttttg
     5041 caggatatcc tgcaattcac agcagtgaac ggtacgacca ataatacggc ccagcgtgga
     5101 gtgcagctgt gccacttcca gcgtgttgcc agtcagtttc tgataaatcg caacgatttc
     5161 attcagtttg ttctgggtag attcgcgacc tgccgccagt ttcaccagca tattagccag
     5221 cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg aatatttccc
     5281 gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggtg cctgcggcgc
     5341 ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca ggtattcatc
     5401 ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt agccgcccgg
     5461 gaacaggaag ctgccgtttt tactgtcggt cgggaattcc ggcacgctca ggtagttcac
     5521 cgcaccttta ccgcgcgtca gccattccgg gtagaacgca gcgataaccg cagtatcgac
     5581 cttgtaaacc tgctcaacaa agtcactcag cttgtcgatg aaagacttga tgtacatcag
     5641 gcgctcaagg ttcagcacgc ccagaccgtc gaggttgatt gggttagcga ccccacctac
     5701 cgccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggttagcgtc
     5761 acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca cttccggcgg
     5821 cagtttcatc gccgggtgac cccagtagcc attggcgaaa atacccaact gaccgctggc
     5881 aaccagatct ttgatcttgt tctgaacttt ggtgaactct tccggactgt tcaggtgcca
     5941 ggtcgaaaca cctttcagca tttcggaggc tttggttggg tcagcttgca gtgcagaagt
     6001 gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt catgcgtggt
     6061 gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag
     6121 cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata cgccacagat
     6181 acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc
     6241 gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc
     6301 gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct
     6361 ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga
     6421 cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga aatcaacagt
     6481 tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac cagcgaatag
     6541 gtcagacgcc aggttgcaca acctaacagc gcgctcagtg ctgacaggaa cagcatgcgg
     6601 gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact
     6661 tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga
     6721 tagataagct cgccaaagcg cagcacgatg aaaatcgcca gcaacacact gatggtgttg
     6781 gtcagcttga cgaataggct cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc
     6841 agtgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac
     6901 agcggcaaca tttcatagct ctgccacagc ggatgcacct tgtagcccgc cgagatcatc
     6961 agcgacccca ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc gatgatgaag
     7021 aacatcacct tgttcagatg ctgtagcgac accttccagc ccagacgttc aaacagtgcc
     7081 ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc cgtctcgaac
     7141 agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga
     7201 cccacgtcga tagtgatcga caagccaccc agtgagtaac caaacagact cgccaacagc
     7261 gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgcaagc
     7321 gcccagccgc cacaggcaaa gccggtgcca atcagcaggt caaacgcgat ccacacgccc
     7381 cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca
     7441 atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt
     7501 ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt
     7561 tttggtgttg cgacgaacca gcacggtcaa gcccgccagc acagccagtg gtagcatcat
     7621 gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag
     7681 tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct gagtaccgcc
     7741 gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca ggtaagtgtc
     7801 gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca gcgccagacg
     7861 ttttttcgcc tccgccatca gctcttcacg cgtaccaaaa atcaccgcgc ccgccgggca
     7921 cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt tctggttgca
     7981 cagctcgcac ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg gcacgttgta
     8041 cggacaggcg accatgcagt aacggcaacc ggtgcacacg tctttgtcgt aatggacaat
     8101 gccggttttc ggatcttttt tcagtgcaga gaccgggcac acagagacac agttcggatc
     8161 gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt
     8221 gacccctgtg ccgctggtcc acacttgaat gatgttattg gtatacggcg acagtttgtc
     8281 gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaagttga tatcctgaca
     8341 cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca tccccagcga
     8401 tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca gcgcgcccgt
     8461 cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc
     8521 cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg
     8581 acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc agagacgttg
     8641 ccgccctctt tagcgttaac atccggtttc tgtgaacgcg gagtctgatt ttcgacgttg
     8701 gcaagctgat ggatgccttt atggaagccg ataccttctt cgttacagcc atagcacggg
     8761 tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt tgagcagttg
     8821 ccgtaagttt ctggcccttt acagccgagg tggtacaggc accagccttc gcggtggcct
     8881 tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg ttcgcagtgt
     8941 tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggt
     9001 ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag
     9061 cccggaatat tgataacggt tttgcctggc agaacttctt gcaagctgac tgcgccagtt
     9121 gggttaactc cagctgcggc aacaccgccc cacgcagagc aggaaccgat agcgataatg
     9181 gctgctgcgc cttccgccgc tttgcggatg tgatccacaa tcggctcacc ggcaaccatg
     9241 caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac atactgccct
     9301 ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa
     9361 agcacttcgt gatactccag agagatagtc tccagtacga ggttttctac cgttggatgc
     9421 gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact
     9481 ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac
     9541 cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga
     9601 gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgaa gacctggcat
     9661 atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac cacacattta
     9721 ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga agaagcatta
     9781 gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg cgataaccgg
     9841 ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct
     9901 ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat
     9961 ctccggtctt ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt ttataatcgg
    10021 cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta
    10081 agcctttacc aaaatggtta ttcatcgttc acctcattcg ctatatatcg taatatatag
    10141 cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatgctt gtttaagacg
    10201 taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt cagtgtcctt
    10261 tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg
    10321 tgattaaccc ccggatagat atacgcctca taaaccttat tattggcttt caacgccgcc
    10381 tcgtaagcag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg gagtagtaaa
    10441 ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcactgcg
    10501 caggccagtt ccgggtaagc gaccgctgcc gcgttcgata cgccaccgcc atagcaaaat
    10561 ccggtaatac ccactttgcc tgtcgcttgc ggatagcgtt gcataaactc aattgcggca
    10621 aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca
    10681 tcatttcccg gataacctcc aacggaattt aagccgtcag gtgccagggc gatatacccc
    10741 gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg attctcatgc
    10801 accaccacca cggctggcgt tttgccgctc atttttgcgg gtttaaccag ataaccccgt
    10861 acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaaaaat ctccgggtcg
    10921 gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa caacgctaat
    10981 gccgtcatcc cgcctaccgc atacttcgcc gcaaggttga ggaactcacg tttcgagatt
    11041 ttcccgtgag cgtaatagtc gtagtaatcc agcaactctt gtgggaaatc tttggcggtt
    11101 aaacgcggca tcgttgcact cctcagttgg tgttttttta agcaaagcat aagcacgtgt
    11161 ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc
    11221 taaactagaa acattgtttc gaaattgaac ggtggaaagg agaggtcatg gtctggttag
    11281 cgaatcccga acgttacggg cagatgcaat accgctattg cggaaaaagc ggtttacgcc
    11341 tgcccgcgtt atcgctcggt ttatggcaca atttcggtca cgttaacgcg ctggaatcac
    11401 agcgtgcgat cctgcgtaaa gcgtttgatt taggcattac gcactttgat ttagccaaca
    11461 attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg cgggaggatt
    11521 ttgccgctta tcgcgatgaa ctgattatct ctaccaaagc tggctacgat atgtggcccg
    11581 gcccttacgg ctctggcggt tcacgtaaat acctgctcgc cagcctcgac caaagcctga
    11641 agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat gaaaatacgc
    11701 cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag gcgctgtatg
    11761 tcgggatctc ctcttactca ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt
    11821 ggaaaattcc gctgttaatt catcaacctt cgtacaattt actaaaccgc tgggtggata
    11881 aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgcattgcc tttactcctc
    11941 tggctcaggg attgctgacc ggaaaatatc tcaacggcat tcctgaagat tcacggatgc
    12001 atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat gctcaccgaa gccaacctca
    12061 acagcctacg cttattgaat gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg
    12121 cgttaagctg gttgctgaaa gatgagcgag tgacgtcggt attggttggt gccagccgcg
    12181 cggagcaact tgaggagaac gtgcaggcgc tgaataatct gacatttagc accgaggagc
    12241 tggcgcagat cgatcagcat atcgccgatg gcgagctgaa tctgtggcag gcgtcttccg
    12301 ataaatgacc tgttaataac gggcgcgaga accgcgcccg aatcagatca gtgattatga
    12361 cgagtcagtc ggtcaagata gcccattaca aatgcagaga gcacaaacgt cagatggata
    12421 atgacgtacc acatcagttt gttatcaggg acatttttcg catccataaa gacgcgcagt
    12481 aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag cgacgttgca
    12541 tccattttcc ccagccagtt cagcttctct ttgttctcgg agatatcgag ttgtgagacg
    12601 aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag cgtcatatcc
    12661 accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat attcggcagt
    12721 acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag cgaaaggcca
    12781 aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag aaaacgttcc
    12841 atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag taaacagcgc
    12901 gcaacagcaa atcaacatat ccgttaaatc tttgtcgcat aattctgagg gttagggatc
    12961 ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt tgaacctgaa
    13021 gggagaaaaa cgatgtctca tttaaaagac ccgaccacgc agtattacac tggtgaatat
    13081 cccaaacaga aacaaccgac gccaggcatc caggcgaaga tgacaccggt accggattgc
    13141 ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagccct ggtgacaggg
    13201 ggcgattccg gaatcggtcg cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg
    13261 gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa
    13321 gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa atttgcccgt
    13381 tcgctggttc acgaagcgca taaggcctta ggtgggctgg atattatggc gctggtcgcc
    13441 gggaaacagg ttgccattcc tgatattgca gacctcacca gcgaacagtt tcaaaagacc
    13501 tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg
    13561 aaaggtgcaa gtattatcac cacttcgtca atccaggcat accagccaag cccgcattta
    13621 ctggactatg cagctacgaa ggcggcgatt ctgaactaca gccgaggcct ggcaaaacag
    13681 gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag gcccgatctg gacagcactg
    13741 caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagca aacgccgatg
    13801 aaacgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc aagtcaggag
    13861 tcgagctacg tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa
    13921 aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat tcactcttac ctgatgtggc
    13981 gaaaacgcct tatccgccta caaatgcatg aagattcaac atgttgcaat agattttgta
    14041 ggcctgataa gcgtagcgca tcaggcattg agcacctaat gccggatgcg gcgtgaacgc
    14101 cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga
    14161 tgaacgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct tcgccgacca
    14221 gacctatctt caggtaaccc gcctgatgca gcgtgtccat taccttcatc aacgtctcgt
    14281 aatcgacagt tttatccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg
    14341 cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt
    14401 tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag
    14461 aagcaggcaa gttaaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa
    14521 tcagcaggac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt
    14581 tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgcg cgtaattttt
    14641 gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca
    14701 gcaacaatac ctgcgctgcg acatcaccca gcatcgcttt aaagccgcca atctgacgcg
    14761 cgaatacgtt atagataacg accgccggaa tcgctgccac cagaccgatt gccgttgcta
    14821 acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga
    14881 taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagcccaacg aacggcgaaa
    14941 tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgacct actgcggcga
    15001 cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt
    15061 ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac
    15121 caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg cgcttcagcc agcagttgct
    15181 gctcgcgctt aaggcgacgc ttctgattaa agaactctac gctcttactg aagaagattg
    15241 cccaggtgac tacggaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg
    15301 cgtgctgata cataccccag acggaaaggt ccgtctgcat taaattatta cccactgtgt
    15361 atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaacgac gtcgaattga
    15421 tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta
    15481 cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt
    15541 caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat
    15601 cccgacatgg cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa
    15661 tacactctcg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt atttgagagt
    15721 atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg
    15781 cgcggaacgt taacccattt ttccttacaa caagcgatgt gtgaactgga aggtggcgca
    15841 ggctgcgcgc tatttccctg cggggcggca gcggttgcta attcgattct tgcttttgtc
    15901 gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt
    15961 agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggtgcc
    16021 gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc gccgggttcc
    16081 atcactatgg aagtccacga cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg
    16141 gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat
    16201 tttggcatcg atgtttctat tcaagccgcc accaaatatc tggttgggca ttcagatgcg
    16261 atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaatgcctat
    16321 ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca
    16381 ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggccga atggctggca
    16441 gaacatccgc aagttgcgcg agttaaccac cctgccctgc ctggcagtaa aggacacgaa
    16501 ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa
    16561 ctcaatgatg aagagctggc gaactatctg gataacttca gtttattcag catggcctac
    16621 tcgtggggcg ggtatgaatc gttgatcctg gcaaatcaac cagaacatat cgcagccatt
    16681 cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa
    16741 gatgtcgacg atctgattgc cgatctggac gccggttttg cgcggattgt ataacattgc
    16801 cacttttgga caattttgca gacattttat tgtgaaaagt cttaaattgt tgcgtccggg
    16861 atcaaggcgt cccggacgat tcaggagtac aataggcaga taaaggctta aacgctgttc
    16921 cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt
    16981 tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct
    17041 gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatatt
    17101 ggctggcgca ttgattgccc agggggttat ggattttctg cctacgattg cgattctgac
    17161 cgccgcagca agtctgggct gctggctaag ttatattcag gggcgctggt tagggaatac
    17221 caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcaccagc gcgccacctg
    17281 catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg
    17341 tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgtt tccagttttt
    17401 caactggtta agcggattgc tgtgggtcag cgtggtaacc agtttcggct atgccttaag
    17461 tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc
    17521 aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtggtgatta aaaaaaaata
    17581 ctgtaacgcc tgacgatttt ccctgttccc ggttgctgta ccgggaacgt atttaattcc
    17641 cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa
    17701 actcacggct aaattgcgat gcgctttcat agccgacgcg catcgctgct gcgctggcct
    17761 tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact
    17821 gcaacggcga ggtgctggtg acagacttaa aattatggtg gaacgccgat acgctcatat
    17881 tagcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaatccgtt
    17941 tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc
    18001 cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa
    18061 tacgcgcatc cagtggtcgc gccatcacgt cgagtaaccg ctccgccgcg cataaaatct
    18121 cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat
    18181 cttcgccaat atccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc
    18241 ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca
    18301 gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat
    18361 gtccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg
    18421 tctcgccata caacaaacgc acatcgggca acagttctga caaactattt tctttatttt
    18481 tcagtttatt aactttatcc gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta
    18541 atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac
    18601 tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat
    18661 cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc
    18721 aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa
    18781 tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggtggcg
    18841 gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg
    18901 tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga
    18961 aactggttcg cgaacagaaa gtgactttcc tgctgggggt tggcggcggt tctgtactgg
    19021 acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca
    19081 ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt gtgctgacgc
    19141 tgccagcaac cggttcagaa tccaacgccg gtgcggtgat ctcccgtaaa accacaggcg
    19201 ataagcaggc gttccattca gcccatgttc agccggtatt tgccgtgctc gatccggttt
    19261 atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca
    19321 ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag
    19381 gcattttgct gacgctgatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg
    19441 atgtgcgcgc caacgtcatg tgggcggcga cgcaggcgct gaacggtttg attggcgctg
    19501 gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc
    19561 tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgata
    19621 ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact gaaggttccg
    19681 acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg
    19741 tgcctaccca cctctccgac tacggtctgg acggcagctc catcccggct ttgctgaaaa
    19801 aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg ctggatgtca
    19861 gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg
    19921 catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt gagttcatat
    19981 aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc
    20041 ccagttggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa
    20101 agcgttagaa gtgggttatc gctcgtttga taccgccgcg gcctataaga atgaagaagg
    20161 tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca tcaccactaa
    20221 gctgtggaac gacgaccaca atcgcccccg cgaagccctg ctcgacagcc tgaaaaaact
    20281 ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta
    20341 tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg
    20401 cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg gcgtgacgcc
    20461 tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac acgcctggaa
    20521 cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt
    20581 tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaaaccc cggcgcagat
    20641 tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc
    20701 acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcga
    20761 aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt tcggcggcta
    20821 acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg gacttttcag aaatcattta
    20881 ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac
    20941 atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc
    21001 aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt
    21061 ttaccgtgca cgcacagccc ccctcacaga ccgtagaaca aacagtccgg catatttatc
    21121 agaactataa atcagatgcc actgcccctt attttggtga aaccggagag cgtgcgataa
    21181 cttctgtgcg tattcaacag gcgcttaccc tgaacgacaa tcttacgctg ccgggtaata
    21241 ttggctggat tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgctagaaag
    21301 cgtagcgata accgaaactg acgccgatca tgccgatgcc gttgtgcgct ttcgtatctt
    21361 taaagatgat aaagaaaaga ccacgcagac actgaaaatg gtggcggaaa atggtcgttg
    21421 ggtcattgac gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa
    21481 tgaaaaaacg ctggccgctt tagcttcgtt gcaaaaagaa cagccggaag cctttgttgc
    21541 cgaactcttt gaacatattg ccgattatag ctggccgtgg acgtgggtgg tttccgactc
    21601 ttaccgccag gcggtcaatg ccttctataa aaccaccttc aagacggcca acaatcccga
    21661 tgaagatatg caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga
    21721 gtcgctattt tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg
    21781 cattcatatt cgttttacgc tgaccaatgg caacaacgaa gagcaagaac tggttttaca
    21841 acggcgcgaa ggtaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact
    21901 taagcagatt gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaatagcaa
    21961 ttagccggaa caataaaaaa gggaacacta tatgaaaacg attttcaccg tgggagctgt
    22021 tgttctggca acctgcgtgc tcagtggctg cgtcaatgag caaaaggtca atcagctggc
    22081 gagcaatgtg caaacattaa atgccaaaat cgcccggctt gagcaggata tgaaagcact
    22141 acgcccacaa atctatgctg ccaaatccga agctaacaga gccaatacgc gtcttgatgc
    22201 acaggactat tttgattgcc tacgctgctt gcgtatgtac gcagaatgat aaaaaaatcc
    22261 ccggcagcat gtcagttgcc ggggattttt tttaacgtcc aaccgccgct ttagggcgtt
    22321 tcttcgcacc agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag
    22381 gcgtctgaca ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg gtattgcggt
    22441 tctggcgacg agcctcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac
    22501 ggctgccaat cagatgcttt ttgcccatcg cttccagcgc ctggcggatt aacggccagt
    22561 ttgccggatc gtggtaacgc aacaacgctt tatgcaaacg acgctgtttg tcgcccttcg
    22621 gtacgaagac gtcttcactc ttataaccaa tcttcgccag cgggtttttc ccggtgtaat
    22681 acatggtggt cgagttagcc agtggcgatg ggtagaagtt ctgtacctgg tcgagacgga
    22741 aacgatgctt tttcagccac agcgccaaat tcaccatatc ttcatcacgc gtaccggggt
    22801 gcgcggagat gaaatagggg atcagatact gctctttacc tgcctgtttc gagtaggtat
    22861 cgaacagctc tttaaagcgg tcataactgc ccatgcccgg cttcatcatc ttcgacaacg
    22921 gcccttcttc ggtatgttcc ggagcaatct tcagataacc gccgacgtga tgggtcgcca
    22981 gttctttgat atagcgcgga tcttccacgg cgatgtcata acgtacgcct gaggcaatca
    23041 gaatcttttt aatgcctttc agatcgcgag cgcggcgata gaggttgatc gtcggttcgt
    23101 ggttagtgtc catatgcgga caaatatccg ggtaaacgca ggacaaacga cggcaggttt
    23161 gttcagcgcg tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat
    23221 cggaaattac gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga
    23281 tcgaatcttc ggaacggctc tgaataatgc gcccttcgtg ctcggtgata gaacagaaag
    23341 agcagccgcc aaagcagccg cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg
    23401 gaatacgggc attgccatag gccggatgtg gcacgcgctt gtacggcagc gcaaaaacgc
    23461 tgtccatctc ttcggtagaa agcgggatag caggcgggtt gatccacaca tagcggtcac
    23521 cgtgtttttg catcaatgcg cgggcgcaac ctgggttagt ttcgtggtgc agaatacgcg
    23581 aagcatgggc gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaacacgt
    23641 aggttttttc ccacggtttc gggcgcggtg gctgcacggt tacggctttg gcttcctgct
    23701 ttttcggtgc caccggtttg ttatccgcgc acggcaaatc ttcaccatac ggatgcggga
    23761 ttgggtcgat ttttccaggg gtatcaagac gggtggaatc cacgccgctc cagccaggca
    23821 gcgcctcttt cacgataatc gcggtattac gcacatcgcg gatttcacta atcggctcgc
    23881 ccatcgccag acggtgcgcc acctccacca gcggacgctc accgttacca aacatcagca
    23941 tgtcggcttt cgaatccacc agcacggaac ggcgcacggt atcggaccag taatcataat
    24001 gcgcggtacg gcgcagacta gcctcaatac caccgaggat caccggtaca tctttccacg
    24061 cctctttaca acgctgggta taaaccagtg tggcgcgatc cgggcgctta cccgcgacgt
    24121 tatccggcgt gtaggcatcg tcatgacgta aacggcgatc ggcggtataa cggttgatca
    24181 tcgaatccat gttgccagca gtaacaccga aaaacagatt cggtttaccc agacgcatga
    24241 aatcgtcttt gctgctccag tctggctggg cgatgatccc gacgcgaaag ccctgtgctt
    24301 ccagcatacg accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag
    24361 taaccaaaat gatgtcgcag ctatcccagc caagttgatc catctcttca cgagacatcg
    24421 gcaaaaacgg tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc
    24481 gatccggttg gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat
    24541 caaaggccgg ggattataag ccggaacgaa agagaaatcg aaaagtattc catactcgcc
    24601 ctcctcgggc gagtatgaag attacggtac cggattgact aacagttgcc caatcgagcc
    24661 acggtccgcc atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg
    24721 ctgaccgaaa taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc
    24781 tctgtcttcc gggaacggca tcgcaccatt cacgttacgg atctggaaca tcacgccttc
    24841 aatatggaac gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg
    24901 cgcggtgaca tcaatacggt tgacgtccca cagttgtcca ttaatacccg ggtcatcacc
    24961 cagactgata tcgcgactgc gaatcggcga accggccatg atttcagttg gcagcaagcg
    25021 catcggaaga ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccag
    25081 ggtagaaacc agaatgctgg atggctcaaa gaagccacga atacgatcaa caatgctcgc
    25141 cgcttcgcca caggtgatcg acacttcatc accgttgctc atatccacca gaatctcgcg
    25201 gcgctcgccc ggtgccaacg aaagttgctt caccgataca ggagcaggca ggaatccctg
    25261 atcgccagaa atcacatgta acgggcgacc atcgctcatc tgtagttgat agcgacgaga
    25321 gttcgacgcg ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct
    25381 ttgtacaccg ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta
    25441 ttctggcgta ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac
    25501 accataatgg ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc
    25561 ggcaaggccg ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag
    25621 agtagctgcg ttctggcgaa taggcagtac gggtgcccag tcagcgtttg gcgacatcat
    25681 ccgtgccgga ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac
    25741 attttctgtc aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat
    25801 agtcggcccc aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa
    25861 tgaccagtgc gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagcag
    25921 cggcggaacg ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc
    25981 tgcacaaagt gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc
    26041 tccatgtaaa actggctaaa gtaataccgt gcaggaaagt gtttcccgca aaaatacaga
    26101 ttgccgactt aaacttttcc ggcggcttcg cgctctgcga cttctttatc gagctcggcg
    26161 attttttgtt ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca
    26221 tactgactga cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga
    26281 ttaagattaa tcttattcga agttgtggag acgcacacgg gaataatcgg gacgcccgcc
    26341 gcaattgccg cgtgaaatgc cccagtcttg aacggtagca ggccgcgacc acggctgcgg
    26401 gtaccttccg ggaacatcca gatggaaata cggcgttttt tgaaatgatt cactacttcc
    26461 gcaatagtgc cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac
    26521 cagtacaact gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc
    26581 ggcggttgca caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata
    26641 tagatagcat tgccgtagct ttcagcatct gcaggtttgc ggcactcaac tttcaggccg
    26701 aacagcggcg caagacgacc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc
    26761 gggctgaaaa ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta
    26821 ataataagac gaaagatata tagcataaca ccctctgaag gtctgagaga ctggcattgt
    26881 acgtcacctg aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt
    26941 taatcgcaac gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg
    27001 aatacgctgc cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa
    27061 cagcgccctc cccggcatat tactcttcgc tatcaccgct gctggcacgg cgaggagagt
    27121 cgatctcaac acgatcgata cgctgcaaac cgcgcatcaa cgtaccgcgg cgtccacgtt
    27181 cgccagtgac tttctgtagc tcttccggac gcagtttaat tttgcgtttc ccaacatgaa
    27241 tggtcagcgt gctttgcggc ggcagaacgt acagttgcgc cagaccatcc tcgccacgcg
    27301 cggcttctgc cgatggaatg ttgataatct tgttgccttt gcccttcgac agctgcggca
    27361 gatcacttac cgggaacatc aacatacggc ctgcctgagt gattgccagc agcatatcgg
    27421 aagcatcttc aattaccacc ggcggcataa catgggcatt ttccggtaag gtgatcaaag
    27481 ccttacctgc acggttacgc gccaccagat cgttaaaggt gcagacgaaa ccgtaacccg
    27541 catcggaagc catcagcagt ttctgatcgt cgctttccat cagcatatgg tcaacggtcg
    27601 ccccaggcgg caacgttaat ttgccggtga gcggttcgcc ctgaccacgc gccgacggca
    27661 gcgtaatcgg atcaatggca tagctacgac cggtggaatc aacaaacact accggttggt
    27721 tgctcttacc tttcaccgcc gctttgaagc tatcacccgc tttataattc aggcccggcg
    27781 cgtcgatatc atggccttta gcgctgcgta cccagcccat ctgcgacagc acaatggtga
    27841 caggttcaga cggcagcatg tcgtgctcgc tcatcgcttt cgcttcttcg cgttcctgca
    27901 acggcgaacg acgatcgtca ccgtaggctt gcgcgtctgc ctgcagttct ttcttcagca
    27961 ggttattcat tttacgctcg gaagccaaaa tgccctgcaa ctggtcgcgc tctttttcca
    28021 gctcactctg ctcaccgcga atcttcatct cttccagttt ggcaagatga cgcagtttca
    28081 gttcgaggat cgcttccgcc tgggtttccg taaggccaaa ccgcgacatc agcgccggtt
    28141 tcggttcatc ttcattacga atgatctcaa tcacttcgtc gatattgaga aacgccacca
    28201 gcaaaccttc gaggatatgc aggcgcttga ggactttctc cagacgatag ttcagtcggc
    28261 ggcgcacggt atcgcggcgg aacaccagcc attcggagag aatttccagc aggtttttca
    28321 ccgccggacg accatccaga ccgatcatat taaggttaat gcgatagctc ttttccagat
    28381 cggtggtagc gaagaggtgg ttcatcacct gatccatatc cacgcggttg gaacgcggca
    28441 caatcaccag acgggtcggg ttctcgtggt cagattcatc gcgcagatcg tcaaccatcg
    28501 gcagcttttt gttgcgcatt tgcgcagcaa tttgctccag tacgcgcgca cctgaaacct
    28561 gatgcggcaa tgcgctgata accaccgcgc catcttcttt cttccacacc gcgcgcatac
    28621 gcactgaacc acgtccgttc tcgtagattt tacggatctc ggcgcgcgaa gtgataattt
    28681 ccgcttcagt cggataatcc ggcccctgca cgatatccag cagctgatcg agcgtggttt
    28741 tcggctggtc gattaatgcg attgccgcct gagccacttc acgcaggtta tgcggtggaa
    28801 tatcggtcgc catgccgacg gcaataccgg tggtgccgtt aagcaaaatg tttggcagac
    28861 gggcaggtag cattttcggc tcctgcaaag tgccgtcgaa gtttggcacc cagtcagccg
    28921 tcccctgccc caactcgctc aatagcagct cggaatattt cgacaaccgg gattcggtgt
    28981 aacgcattgc cgcgaacgat ttcggatcgt ccggcgcgcc ccagttcccc tgaccatcaa
    29041 ccagcggata acggtaagag aacggctgcg ccatcaggac catcgcttca taacaggcgc
    29101 tatcgccgtg cggatggtat ttacccagta cgtcaccgac ggtacgggcc gattttttaa
    29161 atttggcgct ggcattcagg cccagttcag acatcgcata cacaatgcgg cgctgaacag
    29221 gtttcagacc atcaccaata aacggcaacg cacggtccat gatcacgtac atggagtagt
    29281 ttaagtaggc gttttccgta aattcatgta gcgcaaggcg ctctgccata tcgctcatta
    29341 attctgattc ctcaacttat tcgccagcct tcaaaggggc aatattgccg gagatactac
    29401 attatctgcc gcgtcgagtc acaggagatc agcgtgaata gcgccgccgg acgtgctccg
    29461 acggcgataa aggcatcaat gtgccttgat atacaactct ttgctgtaga cataatcctg
    29521 gggattatgc agcggaaaac cgccaacgta gggtttaagc agtttgatta acggctgata
    29581 gtagatagga atcagcggtg cctgctggtt gacgatcact tctgcctgct gatacaacgc
    29641 attacgcttt gtcgcatcag tgatctgcat ggcctggttt agtaaggcgt catactgcgc
    29701 atttttccag tgaccgacgt tttcttcact atcgcttttg agcgtgttca ggaagctgga
    29761 agcatcattg tacgtcgcat cccacgactg ccgagacagc atgaaatcac cggctcgtcg
    29821 ggcatcaaga taggttttcc actccattgt gcgcagcgtc acctgtgcac ccagccattt
    29881 tttccattcg gaagacaacg ctatcgcggt cttttcatgc agatcgtact tgttgtagaa
    29941 cagctcaaag cgtagcggat gagaggcgtc gtatcccgcc tgtttcagca aggcttttgc
    30001 catcgcgacg cgctcactca ttggcttttg cagttcatcg aacgtcgtcg cgctaaagcc
    30061 ttttacctct ggcggcgtca gcgtggttgc gggcgttctc aaccccagta ccttttgcgc
    30121 aataagctgt cgatcaaccg taagatatag cgcccgacgc actcgcacat cgttaaatgg
    30181 cggtttctca aggttgaagt tgtaatattc gctgttcaga cgcggaataa ttcgtatctc
    30241 gccaggcagt gatttttcaa tggcgggaat ttgctgcgcc ggaacccagg tgagatcgac
    30301 ctctcccgcg cgatagcggt tatagccggt gaccgaatta tccagcgcca gatactcaac
    30361 ctgttgcaat actgtatgtt gcgcatcgcg gtactttgga tttttgcgtg cagtaatctt
    30421 ttcgttaact acccactgat caagcacaaa ggcaccgttg taaaccatgt tctctggctt
    30481 actccagcta tcgccatgtt tagcgatgac atgatgagga accgggaaca gcgtcggcca
    30541 ggcgagcatc gtcgtgaacc aaggaaccgg ctgctcaagc gtaacttcaa gagtacgatc
    30601 atccgtcgct ttgacaccca gcgatgtaac atctgcttta cccgcaacaa tagccgcggc
    30661 attgttaatg tgcgcctgtg ccagatagcc agcaaaaggg cttgccgttt tcgggtcaac
    30721 cgcgcgctgc cagccgagga caaaatcctc tgccgtcaga ggctgaccgt cggaccactg
    30781 caaaccgcta cgcagatgga aaatatagcg cctgccgccg tccagtatct cccagcgttc
    30841 agcctgagcg ggctgcacct ggccttcacc gtccatccat accagacctt caaacagatc
    30901 cagcacaatc tgcgcggcag tattctcctc caccttttgc gggtcgagcg tacctgggtc
    30961 gctatgattg ttgtaacgaa agacttgttg cggggcgagc tgtgtgttgg tgggaacgtc
    31021 agcagcgtag agaggagccg cacttaccag gctgaccagc cataataaat ttcgcgtata
    31081 catggtattc cttaccggag aaattataag cccaacaaca atacgcctgt ggcattgttc
    31141 gctcaaactt atcgcgagtg atttggctca cactccggta aagattcccc atcacgctaa
    31201 cggcacataa acattcgttt ccagtcgctg ctccgtcacc ccttcggcaa gattggtgta
    31261 gtgaaacaga atcggtgctt tacgcatttt ctcgccgctt gcaggcagcc agtggcgaat
    31321 aatgccccat atcgtgtgtg aaatatcgtc cagctcgcca acgtggcgag ccacggcata
    31381 acgtccaccg gtaagttcac cattgctcac accataacga ttatcgggaa tcggttcgct
    31441 aacgctgccg cagatatcaa agcgaaacgc ttccggtgcg gtggtggcag ggtcgtccca
    31501 ggcgacgcca aaagtctggc tttggttaac aggagaaagc cccgtctctt tacgccacat
    31561 gataaatttc gccacgctgt agttgagcaa ctcaggccta cagcggtgcg gcaacatcgc
    31621 cacagggatt gatgggaaat cgataatgtt tacatccagt gtcaggtttg tcatgttgcc
    31681 tccctgcgaa attattcccg ggaacgcatc ccgggagcgg taacaattac ggatttactt
    31741 tgcggatctg tttgacgtca atttcaacag aattccagtc tttatcgact tcaccctgaa
    31801 tctcaaccgt atctttcggc gtcaccgtca cgccgttcca gcgtttgtgg tcgatatcaa
    31861 cattgatagt accgctggca tctttgaaca cgtagagatc gtcagagatg cgttcaacga
    31921 tattgccgcg cagggttacc caggtgtcgt cacgcaggga ttttgcgctt tctacagtcg
    31981 ttacgctgcc gttcggcccc tggaatcctc cggcctgact ttgcgttgcc gatgggccag
    32041 aaaaaccgcc ctgctctgct gccatcaccg gtgcgctgca cagggccatt actgcgatta
    32101 ctgctgcgaa ttttttcatg tttattactc cctttaatgt ctgtttccga gcatttaaca
    32161 agatagtcct taacaacttc ttaagggaaa aaaataaaat ttagtgctgt acagagcgcg
    32221 ttacaacacg gtttactagc agcaaatacg gttatcgcag ggatgaaaaa atgcgaattt
    32281 tactgataga agatgacatg ctgattggcg acggcatcaa aacgggcctt agtaaaatgg
    32341 gttttagcgt cgactggttt acacaaggtc gtcagggaaa agaggcgctt tatagcgcac
    32401 cttatgatgc ggtgatcctg gatttaacct taccaggcat ggatggtcgc gatattttgc
    32461 gcgaatggcg agaaaaaggt cagcgtgagc cggtactgat cctgaccgcg cgcgatgcgc
    32521 tggaggaacg tgtagaaggg ctgcgtctgg gagctgacga ttatctgtgt aaaccttttg
    32581 cgttgataga agtcgccgcc aggctggaag ctctgatgcg ccgaaccaac ggccaggcca
    32641 gcaacgagct gcgccacggt aacgtcatgc tcgaccccgg caaacgtatc gccacgctgg
    32701 ctggcgaacc cttaacactg aaaccaaaag aatttgccct gctggaatta ctgatgcgta
    32761 acgctggtcg ggtactgccg cgcaaactga ttgaagagaa actgtatacc tgggacgaag
    32821 aggtcaccag taatgccgtt gaagtgcatg tgcatcatct gcgacgcaaa ctcggtagtg
    32881 attttattcg taccgtgcat ggtattggct acacattagg tgagaaatga aatttaccca
    32941 acgtcttagt ctgcgtgtca ggctgacgct aatcttttta attctggcct cggtgacctg
    33001 gctgctttcc agctttgtcg cctggaaaca aacaacggat aacgtcgatg aattgttcga
    33061 cacccaactg atgctgtttg ccaagcggtt aagtacgctc gatctcaacg aaatcaacgc
    33121 ggcggatcgc atggcacaga cgccaaataa attaaaacac ggtcatgttg atgacgatgc
    33181 gctgaccttt gccatcttta cccacgacgg cagaatggtc cttaacgatg gcgataacgg
    33241 agaagatatt ccctatagct atcaacggga aggttttgct gacgggcaac tggtgggcga
    33301 taaagatcaa tggcgttttg tctggatgac ctcacctgat ggcaaatatc gcatcgttgt
    33361 tggtcaggaa tgggaatacc gtgaagacat ggcgctggcg attgttgccg ggcaattgat
    33421 cccgtggttg gtcgcactgc cggttatgtt aatcatcatg atggtactac tgggtcgtga
    33481 actcgcgccg ctgaacaaac tggcgctggc actacgtatg cgtgaccctg actcggaaaa
    33541 accactaaac gcgactggcg tacccagcga agtgcgacca ctggttgagt cgctaaatca
    33601 actgttcgcc cgcacacatg cgatgatggt tcgtgaacga cgctttacct ccgacgcagc
    33661 tcacgaactt cgtagcccgt taacggcgct gaaagtgcaa accgaagttg cgcagctctc
    33721 tgacgatgat ccgcaggcgc ggaaaaaagc actgctccaa ttacattccg ggatcgatcg
    33781 cgctactcgt ctggttgatc aactgctcac gctatcgcgg ctggactcac tggataacct
    33841 tcaggacgtc gcggagatcc cgcttgaaga tctcctgcaa tcgtcggtga tggatattta
    33901 ccacacggcg cagcaggcga aaattgacgt gcgactgaca ctcaatgtcc agggcatcaa
    33961 acgcaccgga caaccgctat tgctaagttt gttggtgcga aatttgctgg ataacgccgt
    34021 gcgttacagt ccacagggca gcgtggtgga cgtcacgctg aatgccgata atttcatcgt
    34081 aagggataac ggccccggtg tgacaccaga ggcactggcg cgaattggcg aacgcttcta
    34141 tcgcccaccc ggacaaaccg ctaccggcag tggacttggg ctatcgattg tccagcgaat
    34201 cgccaagctg catggcatga atgttgagtt tgggaatgcg gaacaaggtg gatttgaggc
    34261 gaaggtaagc tggtaagggt aagacttttg ctaaattcgc aaaagacttt gcacattttg
    34321 ctaatttcac cgtaccgctc tgtgacgtac tatagtcggc aaacgtctca ccttgaggtt
    34381 aaaaaatgag caacatcctg attatcaacg gcgcgaaaaa attcgcccac tccaatggtc
    34441 aactgaacga caccctgacc gaagtcgcgg atggcacact gcgcgacctc gggcatgatg
    34501 ttcgcatcgt tcgcgccgac agcgactacg atgtcaaagc ggaagtacaa aactttctct
    34561 gggctgatgt ggtgatctgg cagatgccag gctggtggat gggcgcgccg tggacagtga
    34621 aaaaatacat tgatgatgta ttcaccgaag gtcacgggac tctgtatgcc agcgatggtc
    34681 gtacccgcaa agatccgtcg aaaaaatacg gttccggcgg cctggtacag ggcaaaaaat
    34741 atatgctttc tctgacctgg aacgcgccaa tggaagcctt caccgaaaaa gatcagttct
    34801 tccacggcgt tggcgttgac ggtgtgtatc tgccgttcca taaagcaaac caattcctcg
    34861 gtatggaacc gctgccgaca tttatcgcta atgacgtgat aaaaatgcct gatgttcccc
    34921 gctatactga agaatatcgc aagcatcttg tggaaatttt tggttaacta gagctcaggc
    34981 tttagaagga gttaaccatg cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac
    35041 atcaccgtca ggcggtattg gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag
    35101 aaggttgcca cggctatgcg ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta
    35161 tggcaccgga ttctatcgtg atgattgagc agtgggaaag catcgcgcat cttgaagcgc
    35221 atctgcaaac cccgcacatg aaggcgtata gcgaagccgt aaaaggtgac gtgctggaga
    35281 tgaatattcg tattctgcag ccagggattt aacattcctg aaaagaggcc ggtgttgcta
    35341 ccggcctcag gtcagattag ccattctttc ttcccgtttt cagcccccga tggaaatcgt
    35401 taattcgctt aatggtttta accgcacgct gcggcaccgc ggaagccacc gaccagacaa
    35461 tcatttccag gcacaacaat accgtaccat gtagtggcgt tttccccttt tcatccccgc
    35521 gcggaacatg aatcacgata ctggcgtctt tgctaaaacg tgaatccagc gcgtttgtca
    35581 gcaaaataac agggatcccc agccgtctcg cttcacgcag cgttgtcagc ccttcccggt
    35641 gcgcagattt ttgcgccatc atgatcagaa catcgccacg ttgaagcgca atcagttgct
    35701 cggcaaggcc aatacccgta cggtttagtg ccgtggcagg caaacctatg cggttgaata
    35761 gtcgtgcggt atagtccgcc agaatacccg aagcgccaat gccaaaaatt gcgacttgcc
    35821 gtgcctgtac caacaacgct accgcttgcg caagcgcgtg gcggttttca ggctctgaca
    35881 ggacattaca ggtatacaaa tgcccttcaa gaacaaagtc gattgcggtg ttaacatcgc
    35941 tcgtcaagtt actcaccgtc gtagacatct tttcgctgga actaagggct ggaccaagcc
    36001 actgttccag agtgcgtttc agatcacgca gcccggcaaa ccccagggcc tgtatagccc
    36061 tgataactgt ggcatcggaa gtgttcaacg tggccgcaat ttccatcgcc gtttgatcga
    36121 gtaccacttc acgatgctca ttaatatagc tggcgacagc tcttaaccca ggcgttaact
    36181 gactcgcgcg agtgcgaaaa cgctcaccat aaatatcaac ccgcgttttg tctttctgaa
    36241 tcatttggcc tccggtaaag gtcgccccgc aatttgtgat tgtatctgcg cagccatcag
    36301 ccccagagat gcaaaggagt tagaaattgc ctcttcacga gaaatcagca cataacgccc
    36361 ggaacgacag gcagtcagaa actgacacca tcccggcatg actttttcca ttgctgccag
    36421 ctcatcctga ggtttaccac cggtgtcacc gcgccaggtt gcaaaaacaa aatcggcatc
    36481 cagttcaggt aaacgctccg cgctcacatc cattcgccca ccttcaggaa tgctctcaat
    36541 cagcggcgga aagcgaaaac cagcatcgcg taatacccgc cccagcgaat gatagctatg
    36601 catcacatta atttttccct gatttgcctg aatcaccgat accgttattt tttggctgtc
    36661 cagcgtcgcc ttcagcgcat tgatttgtgc ctgatagcgg cgttcaagta tcgccagctg
    36721 cgactgcgtt cctgtcagtt ctgccagctt gcgatagatt tctggcgcgc cccctttgag
    36781 atgatcaatg ctcaccgtgg gcgcaatttt ttccagtcgt tcaatgggtg tattacgggt
    36841 cggttctgtg atgatcaggt caggctttgc cgccacaatc gcttcaatat caatatcagc
    36901 ggtgccaata aaagcgatcg atgaattatc aaagtcgaca cctgtcaata acgcgcctga
    36961 tcgaatgaaa tggctaccgt caggccgagt tcgcccgtgg ctggcgacag gaggtacacc
    37021 gagctcaatc agaggaatag tgatatcaag atcgtgtaaa gagacaattc tctttggatg
    37081 agcaggaaca gtgactttgc gccctaaatc atcagtaaag acctgaacag gttctgtcgc
    37141 gcgggcaaag aatgacaaca gtatcagcag agaaaaaaat aaacgcatat aactcccatt
    37201 aaaaactgtt cctgcgctgc cagagcagta gcaaaaagaa tggtccacca atcaatgaaa
    37261 taatgattcc ggcaggcagt tgcaaaggca aaaaagccaa acgaccaatg ctatccgcca
    37321 ggatgactaa caacgccccc gatacggcgc tccccagcag taatgtcgtc tgcccgccac
    37381 gtagaataaa acgtgatata tggggtgcta tcaatccgac aaaaccgatg ttaccaacac
    37441 acgaaacgca gacggctgtc aggattattg gtgcggcgac acgtaacaac gccagacgag
    37501 aactattcac ccctaacccg gttgcaacct gatgaccaag cagagcgata tccgccgtcc
    37561 tggcggtgaa cagcaataaa agagcggctg gcaacatcca acaggctgaa ataccgacta
    37621 acatccagtt agcagcatgc aggcttcccg ataaccacat caatgcagtt tgtacatctc
    37681 gaacatctgc agtggtcata aatacaccga taccggcggc gaaaaaccag gaaacaccaa
    37741 taccaatcag aacaaatcgc gggcgggaaa tttcgcgtgc acaaaaaatc acgattaaag
    37801 ccaccaacaa gccaccagca agaccagcca ctggacgcca gaacattccc agcattggaa
    37861 actggaaaat cagccacagc accgccacgc tacatccttc tttgacgccg attaaaccag
    37921 gatcggcaag gccgttacgc gcaatggatt gcatcgcggc ccccgccata cctaacatgg
    37981 ctccgcatag caatgccatg atgactctcg gcaagcgaat atcctgcaca atataacgtg
    38041 cgtctgtgct tacgttttct ggagcaaaca acgctcgtcc tacttccgat gccgggacaa
    38101 aaaaagaacc atgcatcaga ccgaatatca gtattccaac cgcaaacaga aacagcccac
    38161 caagcaacac cagattttta gggcgtacca gtgtcgaaaa cttaccaaca cgtagtggac
    38221 gtagcccgac gttcattatc cgactcattt gaacattctc gtcgccataa tgacaaaaac
    38281 aggcgcacca actaacgccg tcatgacacc ggtcgctaac tcatggggcg tgaaaagcgt
    38341 ccgagcaata atatctgcca gtaataacaa aagcgcgccg acacaagctg aaagagggag
    38401 cagaacgcgc aaatccgctg ataccagacg cctgacaatc tgcggtacta gcagaccgac
    38461 aaaaccaatg gggccagcaa tcgatacggc tgcgccacag agcaacgcga tcgcaagcag
    38521 agtgaatgtt cgcgtgcgga gtaccgagac gcccagcccc tgcgccatgc gatcacccag
    38581 tgccaacatg ttaagagagg gcgcaaggta aatggctaac acaacccccc ccagagaaaa
    38641 ccaggcggac gtacccagcg tcgcccaatc ctgccccgct aaatcacctg ctagccatgt
    38701 ccgcatcgcc agcagtgttt gttcgtcaag aatcaggatt gcagccgtta ttgaggatac
    38761 gaacgccgat agcgccacgc cgcataaagt aactttcatt ggcgttagcc ctgaacgccc
    38821 tgcggaggaa agcagcaata tcaacaggaa caataacgcg ccgcccgtag atgccagcag
    38881 tgggcgtcca accggaaagg ccagcccaag cgcactggca gcaacgacag caagagcggc
    38941 tccggcattt aagcctaaaa tatgtggctc ccccaatggg ttacgaatga cagcctgcaa
    39001 caaagcacca gcaactccaa gagaagcacc ggtaagcaat gctgccgcta aacgcggcaa
    39061 acggagtctt acaattacgt tgtgatcgaa gttttgcggt tcaaaatgaa gaaatgcctg
    39121 caacaccgtt tgtggtgcaa taaatcgggc accaatccct ggatatacca cgcatcccac
    39181 aagcagtaag gcgaggaaaa ttacagtaac cagccctgtg cgcatcatac tttttctaca
    39241 ccacgaaacg gcatgaagaa cggctttccc gtcagagtat ttattgatgc gtgaacatca
    39301 acgtcaaaga cagcttttac cagctcaggc gtgcaatgct ccccctcatt caacacacgc
    39361 accactttcc cctggcgtaa aaatatcaac gtatcaccat aattgacggc aaaattcaga
    39421 tcatgcaaca ccacgacgac tgtacgcccg tgatgacgcg ttaaatcatg taataactca
    39481 agaatttcaa cctgataacg taaatcaagc caggttgtgg gttcatccag taggatataa
    39541 ggcgtctttt gcgccaacac catcgcaatc cagcaacgct ggcgctgccc accagagagt
    39601 ttttcaacag gtaaatgagc aaactcctgc gtaccggtta atttcagggc ctcttctacc
    39661 gcctgctcgt cggcgtcgct ccattgacgt atgaaatttt gccagggaaa acgcccacgc
    39721 gacaccagtt cataaacggt taagccctcc ggcaataatg gcgactgcgg caagatccca
    39781 agtctacggg ataacgcttt cgttggttgc tcgtgaatcg ctttaccatc cagcaatatt
    39841 gagccgccca tgggttgcaa gacacgtgca atggtgctga gcaacgtcga ctttccacag
    39901 ccgtttgccc caaccagaac ggtcattttt tctgtgggaa cggagaaaga aacaccatca
    39961 acaatgattt tcttgtgata cccggcagaa acattgtcga ggatcagccc ctgccctttt
    40021 gcgttatgtg ccacgtgtac gccaacttaa tcaaagaaaa acaaaaataa aactcattct
    40081 cttttgagag tttgacgcca caaaatg
//
LOCUS       DAZKIF010000027        39176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZKIF010000027 DAZKIF010000000
VERSION     DAZKIF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.027"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(73..405)
                     /locus_tag="ACHOTW_001490"
     CDS             complement(73..405)
                     /locus_tag="ACHOTW_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HFP9843624.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
     gene            complement(1409..1876)
                     /locus_tag="ACHOTW_001491"
     CDS             complement(1409..1876)
                     /locus_tag="ACHOTW_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFP9843625.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            2069..2638
                     /locus_tag="ACHOTW_001492"
     CDS             2069..2638
                     /locus_tag="ACHOTW_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9843626.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            complement(2837..4153)
                     /locus_tag="ACHOTW_001493"
     CDS             complement(2837..4153)
                     /locus_tag="ACHOTW_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843627.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(4305..5078)
                     /locus_tag="ACHOTW_001494"
     CDS             complement(4305..5078)
                     /locus_tag="ACHOTW_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843628.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIGSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKAD
                     VTQQMITLYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            5201..5659
                     /locus_tag="ACHOTW_001495"
     CDS             5201..5659
                     /locus_tag="ACHOTW_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9843629.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            6375..7157
                     /locus_tag="ACHOTW_001496"
                     /pseudo
     CDS             6375..7157
                     /locus_tag="ACHOTW_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            7277..7464
                     /locus_tag="ACHOTW_001497"
                     /pseudo
     CDS             7277..7464
                     /locus_tag="ACHOTW_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7662..8513)
                     /locus_tag="ACHOTW_001498"
     CDS             complement(7662..8513)
                     /locus_tag="ACHOTW_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFP9843630.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(8587..8829)
                     /locus_tag="ACHOTW_001499"
                     /pseudo
     CDS             complement(8587..8829)
                     /locus_tag="ACHOTW_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            complement(9054..10157)
                     /gene="zorE"
                     /locus_tag="ACHOTW_001500"
     CDS             complement(9054..10157)
                     /gene="zorE"
                     /locus_tag="ACHOTW_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HFP9843631.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            complement(10161..10868)
                     /gene="zorB2"
                     /locus_tag="ACHOTW_001501"
     CDS             complement(10161..10868)
                     /gene="zorB2"
                     /locus_tag="ACHOTW_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HFP9843632.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            complement(10875..12527)
                     /gene="zorA2"
                     /locus_tag="ACHOTW_001502"
     CDS             complement(10875..12527)
                     /gene="zorA2"
                     /locus_tag="ACHOTW_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HFP9843633.1"
                     /translation="MLAQLFEQLFQSIDSTLITNIFIWAVIFVFLSAWWCDKKNIHSK
                     FREYAPTLMGALGILGTFIGIIIGLLNFNTESIDTSIPVLLGGLKTAFITSIVGMFFA
                     ILFNGMDAFFFANKRSALAENNPESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLI
                     AQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDF
                     NQNLTEQFGENFKALDASVKKLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIW
                     EECKEIPLAMSELREVLQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGEL
                     LKSGAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNS
                     SETLTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQ
                     LSSNADNMFDSIQTSFDKASAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQ
                     SLGNALLSISKGFVGNYEKLIKDYQIVMGQLQALISANKHRG"
     gene            12739..16098
                     /locus_tag="ACHOTW_001503"
     CDS             12739..16098
                     /locus_tag="ACHOTW_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HFP9843634.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGII
                     MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSLMLENTVDLRVSWKDGSNLKL
                     LLPKPVSGGRFVTNDGSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVA
                     EKFRYLHADLPLLSGRNDKLQQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKD
                     KATLNIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVVDAISLKNPGLHISLLKKD
                     EFAYDLSALNVQDSPWLIVGKLDGTARIAPVIKWMLPVLQTNDLLLNALCEADPEQRK
                     KNFNELIFEIDNNPLQNYCCLLTEYIKKYKMNNGLSLLDLDLFRGISSNYRVVVQLLI
                     SSCLSGDSDTIYDIQEELPFSWGWIPVSIWKDVFQKCWTYLEKQINDKTLALHILQPF
                     IAFMNHRAHIDRRLAPIANMLLTYSESLPTGCDVLPTVSREQFNEAKQMLLRNPDSFG
                     RISIFPKELWSSAITPELKSVFNKLWIEDKYHSRLEKRFNLMLVAALLTQKDNNLIHQ
                     LSALFEFHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            16098..22412
                     /locus_tag="ACHOTW_001504"
     CDS             16098..22412
                     /locus_tag="ACHOTW_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9843635.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            22636..25494
                     /locus_tag="ACHOTW_001505"
     CDS             22636..25494
                     /locus_tag="ACHOTW_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9843636.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            25497..30431
                     /locus_tag="ACHOTW_001506"
     CDS             25497..30431
                     /locus_tag="ACHOTW_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843637.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSL
                     NSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            30431..36772
                     /locus_tag="ACHOTW_001507"
     CDS             30431..36772
                     /locus_tag="ACHOTW_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9843638.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQISSVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            36769..38883
                     /locus_tag="ACHOTW_001508"
     CDS             36769..38883
                     /locus_tag="ACHOTW_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9843639.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
     gene            complement(38993..>39176)
                     /locus_tag="ACHOTW_001509"
     CDS             complement(38993..>39176)
                     /locus_tag="ACHOTW_001509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843640.1"
                     /translation="YHHHGSRDYWSAGNASLFVPAIFVQCVTPSKNHPHPDVTLDIEK
                     TGCTVQYANRISEYNF"
BASE COUNT        10215 a         8874 c         9683 g        10404 t
ORIGIN      
        1 cgctaggctt ttcctagtcc acttttggca caaagctgtt taggtcaaat ctaagctaat
       61 acacttgatt acttacgatt gcatccgcag ccctttcacg ctttctgatg gtaggctaat
      121 cactgtttta atgcagttct ctatgctgcc aatgatgtct tcgcgtgggc cgctgcgcca
      181 gtctgcgctt gcgtaaaaag catcgagaac cgcagtcatg cttttagcgc tgtcaaaggc
      241 gcgaatcaag tagtagcaat ccaggtcatc aagcgagttg ccaaacgaaa caacatcgat
      301 cccgtggctt tggtgtaggg gaacactgat ttcctgcatt atggcgtgaa aagcggcacc
      361 gctgcctttg cgtaaggtgt attgcagtat ttcgacggtt ctcaagcgtt atctccgtgt
      421 tgcccttggg cggctcctag ttgatagcaa taaagtaaac cggactgggc catgaagcta
      481 ctgctaatca gacagtcatc cgtaagcaga agctgcgata tgcggttcaa acgcgctgta
      541 aggctctctg gcgagtgttt taaagaaggt agtcgaaatc atggtgcaga attcgcggcg
      601 ctctcataga gtgactggtg aagccagttg aaacaagcgt agcggagttc gagcaatcaa
      661 tgagaaatct gggcgagctt tagttttacc gcgtcgctta actttggagg ctgattgcct
      721 ctataaggac tgtctcaccg ccacggaaat tatcggatgc aatgcttaag aggggcgctt
      781 ccaaacctag gacatccact ttttcgctcc caacttatcc ggctgcattt acaccgcgaa
      841 cacgttgaac gttagcagct tctggttcgt gtttggttgt tacgcatgtg aggtaaaaac
      901 ggcagcttta cttttgacgg gaacgccata agacaccaaa cagcagagaa gtgtaattgt
      961 taaaaatgtt aaaacataac ttgttcttta agatgtaaac ccttcctcgg gggggaagcc
     1021 ggacatatca accctaaggc catctgagaa gatagcctgt aggtttgctc ggttcccctt
     1081 gccagatgaa gatcaagtct tcctcgtggt gtgacgacct ttcttgaaag atgcattatg
     1141 cgcaaaagaa gctacttaag aatccgccga tagctacttt caccctctgc ttggctgcat
     1201 caaacgcttg cgcgccctac ctaccacaat caacgttacg cagagttccc atcctgacgt
     1261 cttccgctaa acagcggtga tctccaccta cccggaccgg tttaccgcat gaggtaggtg
     1321 tcaggagaga agctagcaca ccatcatttt aaagaaaagt catatatatc atatggctgc
     1381 gttcatattc tgaacgcagt tatcgatgtt aacctaaagg tatgagaaca ctgtcgtttt
     1441 tcatatacaa agggtgcttc ggctcacctg tagcgtttaa gacgaagcac attgtgctct
     1501 ctggcaatag ctgtcggacg gtcgttgagc gtttgaggtg tcttccttca tttccccatg
     1561 cggcgacaac cagtccggca ccgctaacaa gacgtatcaa gtggctatcg ttatctttgc
     1621 caatgggatc agacgctttc atcatttcat gtcgctgggt gtgaacgaat gcgaacaggt
     1681 tggccatgta tacgccaccg tagccccagc ttttggcaaa cgatatgcag cggctcaagg
     1741 tgcgatcatc cttctcggca tcggcgtatg aagggtttag ccctatgaaa agggtatacg
     1801 gttttgattc gtcccagact cgtgataacg agtagcgata cttaccgcac ggtgaaaaaa
     1861 tcgcgtcttt aatcatttcg gttgtcctta tgaagtgatg gtttcagcct aacatgtctt
     1921 cacccgtttt aagcgccaac aaacgaccgt tttccggcat cctccaaccg cacgctttca
     1981 cactgttgga aaattagcct tggcttttcc ggcaggccat cctattttca tcaccgtttt
     2041 ctggcagggg ccggatgagg agatgactat ggctattttt ggctacgggc gtgtttcgac
     2101 atcccagcag gatactgaga accagcgcat ggaacttgaa caagctgggt ggacgttcga
     2161 cttttggttt tctgatgtag tcagcggaaa ggttcctgcg gtgcaacgca aagcgttttt
     2221 tgaaatgctc agcaaaatcc gcgatgggga aacgttggta gttgcaaagc tggaccgcct
     2281 cgggcgtgat gcaattgacg ttctgcaaac tgtcaggacg ttggctgacc gaaacatcaa
     2341 ggttattgtc catcagcttg gcaccactga tctcacctct gctgccggta agctcctgtt
     2401 gtcgatgcta gcagcggttg cggagatgga gcgtgacctg ctgattgaac ggactaatgc
     2461 ggggctgtta cgggcaaaag ctgaaggcag gaaattgggc aggcctgcta aaatcgcacc
     2521 ggaagctcgt ggagcgattt tagacaaaag ggccgctggg gttagtgtta gcgcattggc
     2581 gcgtgaatac ggtgtttcaa gagcgacgct tgctgcatta ctgaataagg gttactgata
     2641 tacttaaaac atgctctaac actttgcaga tacgagcaat acttggtaaa acagcacact
     2701 ttccgccaga atcggatttt tgaggtcctg gcaacagcta attgctgaga acatttcagt
     2761 aaactgttta actcgctatc agccgaacac tgatttgtat agaaatgcta ccctcgttca
     2821 tactatcaaa aataaattaa ttttgatgcc agccaggttg gtcattctca aatacctcag
     2881 cctcggggaa tattttagtc aatgggataa catatttgac cagatcatgg gtaaaaaagt
     2941 catccgcagt ttcatctacc atccacgcag agttctcatc tatgcgtggc acgagataat
     3001 gaacattcag gcggactatt ttagattttt gctcaaaatt tactatggca gcatagtttt
     3061 tttcccttcg tttatcggca acatcctttt gaatatctac atccaaagct atatgcccta
     3121 cgcctagacc ataatccaaa agctggttat ctatctctgc taatgttttt gttacatgtc
     3181 gcgaacgtgc atcgaaagat ttttcattga tacaacgcca tgttatcaaa gaagcaagtt
     3241 taactttggt tatgaaacgt gagtcgcgct catctggtcg tcccatagca acaacttggt
     3301 aattttcatc ttccagaggg gagcctttga taagacgagc aagtttagtg ttgactagta
     3361 atgagccgtt ttcagtgata tcttccctga taagatttaa gttggccggt ctaatcgttc
     3421 cttttccata atcatcattc cattcataaa atacaccttt aaagtttttt aagtggctaa
     3481 tgatatagtt ttcaggcgcg tctttaactt cacataaata tgtgacatca gtccatacgt
     3541 tcatcttttt ggatttgata tgaagttcgg caagatgtga gcgtatatga tgctgtaata
     3601 tttcctgctt tacgtaagga cctttctgaa gccttttgca ttcgacaaag aaataatcgc
     3661 catctccaag gcggcaacga aactccgggg tttttgctat ccctttttgt tctggaatga
     3721 actcaacttc ataaccttct gatgcatagt ttccagcaag aaccaactct aataaggctg
     3781 tgtctggtaa tactgttgta ttcttaagca ttctgattgc gcgatctcta gcgccagtaa
     3841 ttctatcgag cgatttagca cacactccta attgtttaac ccatggtatt atatttgaag
     3901 cattagtaac ttcgtaagac cttctattat cgataagcga ttttgcttgt gcgaaataac
     3961 cagcaaccac gtcggaacca taccattctg ggtcaaactg tttcccgaaa ttggcagctt
     4021 tttgggttgt agctatgtaa aattgctgtg cttttgctaa gcgctggtaa aagtgttgtt
     4081 tgttctcttg agttgatgct aaccactcaa gaccagcaat aacgtcagaa tctagcataa
     4141 caccgtcatt catctttatt ccttagttca gccctgaaag gcactagatc aactgagaca
     4201 gattatagtt acggaaagat aacgcgatga atatacgatg gtaagcctcc gctttccact
     4261 ctatgctggc agctgagttc tatcatgttc aggcacctac gtttctagaa gttgttcata
     4321 cagttagtat aaatatacgt ccctgaaatc tttgcgtctt gctttggatg ttcatatacg
     4381 tagtcaacaa aagtcttata gagcgttatc atctgctggg tgacgtcggc cttaccgttc
     4441 atgttatcaa gtcgctgata atattctctt ttcgtcatcc cactgttacg gccttcggtt
     4501 acgtttgcgg taaattgagc catacccatg cacatttgat ctttattgtt attcatttga
     4561 tctttattgt tattcgtttt atccgggtgg agagcgcctt ccaacacttt atcaccgctg
     4621 atggcatgtt gcgcccacgc tgaattgtta gcgataaact ttttggagcc gatggcaata
     4681 ccgtattcaa cgcgaattcc attgtcgtga taactgccat ctgcgtcttt tgtcgctagc
     4741 tttaaagata tacggcaacc gggaagcgtt ttgtgttctt gcaccgagat aataacggtg
     4801 tccgtatcgc aatcagcaaa gctgtattcg gggttaacgt aagtgttagt tttgattaac
     4861 agcccggtaa gaccgttttt atagcctttt gccggcagcg ttttgaaggc gctgcgttca
     4921 ccctttttga tcgcttcccg gaagtgatcc ataccgatgc gcgtcgccag ttgatcgatg
     4981 ctttctgctg cataagatgc agatgacgca gcgattaaca taatggtcag cacagacttg
     5041 gctgaaaaac cgggcttact aagccgtgtt tttgacatgt tgttatccct cagaatccac
     5101 cccagcggtg tcagttatcg catcatagac cattgattca aaatgtcact attggagcgt
     5161 ggctctttag tggacatctg cgcaaattca ggtcatgaaa atgaccctga gaaaactgtt
     5221 tggacaacga gtaaaagagt tgcgcattgc caccggaatg agccaagaag cctttgccga
     5281 tcggtgtggt tttgctcgta gctatatgag ccgcattgaa cgcggtggct ctaatgcgtc
     5341 tttggatgcg attgaggtgc tggctaatgc tctgagtgtt gagccgtggc gactattggt
     5401 gtctgattca tctgaagata gtgacccgga attactggtt ccttatgcag ctgatggctc
     5461 gtgttttcat cccgggctgg caagcactcg cgatggctcg tttggcgtag gtgataaaac
     5521 ggctcagaag cggtttggca cgttttctga agctcttgag tatcttcgta gcatggagac
     5581 agcaaaatgg cgtaggccta atcctagcgg caactggggt attgtatctg cggttcggtg
     5641 ggacaagctg agaaagtagc tcaagtgtaa gtttatgaat tttcatgctg tatggatgaa
     5701 cagtgttcat tagatgctga tagcctcacg tttcatcagc tctttgtttc tataagagtg
     5761 aataaaacag caaaatgcgg gtgcgttacg taggcgttac ggtggcgttt tactttgacg
     5821 ctgaatggct taattcatat aaataacatt gagttaaata gttattctga acaaggtctg
     5881 acggggttta actggtttca aaatccgaac gcagtggcgt ttacttaaca tccacatagc
     5941 tgacaaatgt tctttcctcc cgacccacta gacctccata aacatcaaaa ggcaggtatg
     6001 gcacgttaat gatttgtgcc gccacgaaca ctcggtttga ttccgtccag cggatcaaac
     6061 ttaagatgta gcccctagaa caaaaaggtc ctacgattgt tcttttaaag gctaatagga
     6121 acgtgattac agcaggttat ggagacttag gttcgagtcc ggccttcggc accaaaagta
     6181 tgtaaataga cctcaactga ggtctttttt tatgcctgaa atccgcgcca cgcaaggctt
     6241 ttccagcttt tctcctcaac tgaagtcaac ctgaaccaac ccacatcaac aggcttgtga
     6301 gtatactaat gagtatattt gccgattcga tattgcttgt atactcacga gataccaatg
     6361 gaaggaagac cattatggcc ctaacggata ctaaagttcg ctctgcgaaa cctgaggaga
     6421 aagagtattc acttgttgac ggcgacggca tgtccttact tgtaaaacct ggcggttcaa
     6481 agtattggcg atttcgtttc cgttttggcg gtaaacaaca tttgatggcg ttcggcgttt
     6541 acccggatgt ttcactggcg gatgcgcgga agaaaaggga agaggccaga aagctggtag
     6601 ctgctggtat cgatcctcgt gagcataagc gtgctgtgaa agaagagcag gcgaaagaga
     6661 ttattacttt cgagaaggtt gccagagagt ggctcgtaac caaccaaaaa tggtcggaag
     6721 atcatgctaa tcgtgtaaaa aagagcctgg aggacaatat cttcccgaca attggtactc
     6781 gcaacattgc tgaactaggc acccgtgatc tgttaattcc tattaaagcc gtggagaaat
     6841 ccgggcgtct tgaagtggct tcccgccttc aacagcgcac cacggccatc atgcgttatg
     6901 cagtgcaaag cgggttaatt gattacgatc cggcgcaaga gatgtcgggg gcggttgctt
     6961 ccagtaatcg acagcatcgt ccggcgctgg agttaaagcg tatccctgag ttgcttgaca
     7021 aaatagacag ctacaccggc agaccgctaa cccactgtac gacagaactc actctgctta
     7081 tctttattcg gtccagtaag ctgcattttg ctcgctggtc agaaatcgat tttgaaacgt
     7141 caatgtggac catccctctt gattggtatt gcagtaagca gagggttgga ctggtacagt
     7201 aaatatcccg cataatcgtg ccattcacat ttagagatcc tccggcataa tcaatctgcc
     7261 aatgaaggag atcgctattc gtaaagcccc ttttactgag catcagatta tcgccgtgat
     7321 taaatcgctt gaatacgggc gaactgttaa agatgtggct tatctgaagc cacttactac
     7381 aactgcaagt ccagatacgg cggtatggaa ttttctgata ttaaaataat caaggatctt
     7441 gaggatgaga accaacgttt caaatagatg tttgctgatc tcattatttg gtcgggattt
     7501 ttaaagatgt atggaactaa aacgggtcta ttttcagagg gatttaactg aagcagaaat
     7561 taataaatca taatttatag gtggctggta tttaatattc ctgcatcata tctctgatgc
     7621 aggagcagag aatggctata aattattagt aaacagaatg tctatctctc acccacaaac
     7681 ccctcataag ggtaattttt acgcacctgc ggcaacgcaa tagttttccc gtcaaaatcc
     7741 cagaataatc tctcaacgat ccctccgccg tttaccgcat ccgagaccag tacccgcata
     7801 ttttcatcca ggccaatcga tcctttatca aaggctttat ggtgtattgc gcaaagcgcc
     7861 agaccattgg ggatttcaca agggccgccg tgctgtttcc attttatatg cgccgcttcc
     7921 agcgcgacgg tggtgtcatc gtggcgcata ttaaagccac atattgcgca ctggtagtta
     7981 taagcgcgta atacattttt gcgaaataac gggtcgcgct gtttacgggt ttgctggaga
     8041 tcaaaaccca gctcgtcagc gatttcttcc tgaatactct cggtgaagtg cgcttcgagt
     8101 atctgttgcg ccagagtatt gatgagtttt ttattgccgg ttaccagggc gtagtgctgt
     8161 tcatcgaaac cgcctgcaac gtggtattcg ttcagttcct tcactggcgg ttgtcggctg
     8221 ctccctgctg ttgagcaacg ctcggcattg tgtaattgcc agattccgtc gccttgtaat
     8281 cgccagaacg gcatatcagg tcggtactgt gaacgctgtg gcccaaaacg ttccagtaaa
     8341 ctgtgcagag gttcgtagat ttccgtgcca taatcgaaaa ggcgcggatg tccattcagg
     8401 tatcccgcta atacgtatag cagtaacaat ggtttatgcg gcgcgcgctg ttcacccttg
     8461 tgccagattt ttatgttggc aattgcctgc tgtagcgatt tactggaagc cattgatatc
     8521 ccaggcagtg aaataattgc aatcatgctc gcaaattttc tgctgttcaa gcctgcaagt
     8581 gaagatccat tgctgtgttg agggatcaaa agacaacaaa aacttattga agaaataatc
     8641 ttctgctttt ttgtcgaaat aatgggcatt gatcttgtct atagctgctg aatcttttcc
     8701 agcctcgttg agatcttcaa aatagccatt gaagatgttc tggttaacac cgccagtaac
     8761 ataagccttg ccgttgtgta caaaagtcac attacccgcc atgcccatcg gcgcgtgcga
     8821 catcaattta ttgctttatt cattgtgact gtcttctgtc taccaagctt taaccttcac
     8881 ccatatgagg gaagttttaa gtatgaggcg aaccatagca tatgctttta tacctgttac
     8941 accataaaaa tcagacaagt tatgccataa actacttttg taatttgagc cacaaatcag
     9001 ggaaatttta tttatcttcc gtgaaagtct taactttcac ggagttttag aatttacaat
     9061 tttgctggcg taaaggctcc tgtttgtatt tctttaacca gctcacccac ggtatatatt
     9121 ctccatcctt cgacttcggc gggagtaagg ttaactgcaa ttttaaggcc tggccatgca
     9181 gcttctacgg ttgcaataat ttcatgttct gagtttggaa gatctaaccc aggaacagga
     9241 ggttgaaagc ctttatgttg catcatatta atatcaccat gcactgatgg atcagtcagg
     9301 tcatatattt cattccagga ttctgctttt aataaccctt gttgggaacg taaacgttga
     9361 atgatagcca tctctgcttg ggtaacgaat ataccttcgt ggaggggctg ttttcgatgg
     9421 caatccttgc acagaacaag aaggttttca tggtgattat catatttgat gccattttta
     9481 tgatggacat ggcacaagtt tttggcggtt gataaattca cgccacaatc attacaaaca
     9541 taatttgcct tttctcgtat ttctttagat atttccttcc agttttcgac atacccacta
     9601 tttgttttgt catagatacc cttaggcata tgacgaaaac aagtactata atctgaaaaa
     9661 aactcattta atgagaatga tttaaaaatc tctctttttc gttgtttatc aatacttcct
     9721 ttataattaa gtcgttcgag gcaattcata catacccgta aatcaacatc ctggctccga
     9781 cctgaaccat catcaatttc gaataaacca tctatgcggt tagttgcatg atacctttca
     9841 aatcgatttt tatggcgcat ttcatcaaga gttctgcacc aggcaatatg aaaacgtttt
     9901 cctttttcgc catctacgat agccgcatca taacgccctg aatggtcccg aatatataaa
     9961 agaacctggc ggccatgata agagataagc ccgctaccag ggtcaagatc ttcgatacta
    10021 acttctttac ccgtggataa ctcaatatct ataggatcaa agtcaatagg ggattcatcc
    10081 agaaaaaaat cgactccttc tggtaacatt ttcttcccta attgaataag ttctgaaatg
    10141 tcgatagata atttcatctc ttactcctga ataatctttc taatctgcaa ctcggcattt
    10201 gttacaactt tgaaggtgac acgacgagag cgattaggat cctccttgcc ggttttatca
    10261 agaatgggat gtgcagatga ataacctact gcggcaaatt tacttttaac ccattgttgg
    10321 tgtgtcgcga tattttttat gtcataaacg tattgtaata ctgcacgtgt acgaccttgc
    10381 gatagtgcca tattattaaa ataagcaata tcaggattcg ttgttcctgt ccagtcagta
    10441 cttgtgtgac cttcaatgcg gacttcagta atatgttcct gataattatc tagaactttt
    10501 aggtagcgag gaaagaagtc gtcaagaatg agtttaaact ttggttttag ctctgtgctt
    10561 cctaagccaa ataaaacatc cggtgattta aatcgaacct ccagagtctg tttatcgatc
    10621 tctgcatccc agtcttgtaa atcctttgca aactcaatat ccagagcatt ataaagctgt
    10681 aactgagcat tctcgtaggc tacggcaact tctttaattt tttctttttc aatacgtacg
    10741 tagtgcatat aagcaataga tatgaacata aaaaccatca tcagcccagc cattaggtcc
    10801 gacatggata cccaatgttc attatcttga tcttttttag gtaattgttt ccctataatc
    10861 ttatccatga tcgattaccc gcgatgttta ttagcagaaa ttaatgcttg taactgcccc
    10921 ataactattt ggtaatcttt aataagtttt tcatagttac cgacaaaacc tttgctgatt
    10981 gaaagcagag cattacctaa tgattgcatt tcacggttca tttcacgttc agttgcttgc
    11041 tcaaaagcat gtaactgctc gttgatggat agagcaaatt tttcaattga ttctctgact
    11101 tgagaagtca gccctgcact tgccttatca aatgatgtct ggattgaatc aaacatatta
    11161 tcggcattac tggatagttg atggttaatc ttttcaccag tcttactgaa catatcaatc
    11221 acattcgtcg tattttgttc catatttcta tgcaattctt tcgagtgcga ctctagtgat
    11281 ttcaggaatt cttcaccact ttgtttcatt tcagtaattg tttcacctaa cgtgctggtt
    11341 agggtttcgg aggaattgct gacatcatgt gccatcgagg caaatgcttg ttgtgtttgg
    11401 gtaaccgatt gtctgaatcc ttcggtacct tcatccaggg caacgcgcat tgaatctgca
    11461 ttaagaagta tttgctggct ggtttgctca agagatgcac taacatttgc agctccggat
    11521 ttcagcagtt cacccacttc agccattttg ttctgtattt caggtaatac ggttgtagct
    11581 ttatcgcgga tggcgacaaa ggtttctaaa tggcgggaga gttcgctgat ttgatgttgg
    11641 ttcacctgaa gcacttcacg cagttcagac atagccagag gaatttcttt acattcttcc
    11701 caaatccctg caaccgcagt ttttgtttca accagggact cgacactttg ttgatattgt
    11761 tctgacatct gctcaatttg cgttttataa tttccctgcc actcaacaag tttttttaca
    11821 gaggcatcaa gagctttaaa gttttcacca aactgttcag ttaaattttg attaaaatca
    11881 ataatgactt gtcgcaaagc atcaataatt tgttctgtag caccttttgc cattagatct
    11941 gcaaattgtt caagttgttc ccaaagctta ttactgaaat gagtgtgatt agctaattgt
    12001 gcctgcgagg aatcgctaat ctcagtacgt agtaatttta tttgagcaat aagagaacct
    12061 tcactatcac cgttaatacc cgagactaat ttagtcagag tctggttctg ctcttttaat
    12121 tcatgataga tatgttcagg tgtaacagat tcagggttat tttctgctaa cgcacttcgt
    12181 ttattggcaa aaaagaaagc atccattcca ttaaataaaa tggcaaaaaa catacctaca
    12241 atgcttgtaa tgaatgctgt ttttaggcca cctaataata cggggatgct ggtatcaata
    12301 ctttcggtat taaaattgag taaaccaata ataataccaa tgaaagtacc cagaatacct
    12361 aatgccccca ttaaggttgg agcatattct ctaaacttac tatgtatatt ttttttgtca
    12421 caccaccacg ctgataaaaa tacgaatata acagcccaga tgaaaatatt ggtgatcagt
    12481 gtagagtcta tcgattgaaa caactgctca aaaagctgcg ctaacatttt gatacctgtg
    12541 taaataatgg atagttgtaa tcgtttaaaa aataaacaaa acacacataa tttatatggt
    12601 taagttcggt gtagcgctca tgattgtaac aagttttttc gagatataaa tgatttgtct
    12661 catttttagc gagttcatcg ggttgagctt ttttatgatg atagtaacct gttacgaatt
    12721 gtatcaaagg aaggcaggtt gcttgtgaaa actccagtta acccactttt gcaatggctt
    12781 aacatgtttt ttagtcgccg ttcattatcc ggagctgatg gacgggcgtt atatgcttac
    12841 cgttgtactg atacagagta cgaaagtttg gcagaactac tgcgtacata tgccccccgt
    12901 agttatccaa gaacgatatt catttcctat agcgatgttc tatttagcat atatgccgca
    12961 gagtttatcc gccggactca tacggtcgga caccctaaat gggacacaat tttagattct
    13021 attaactgga aagttccgta tgttcatcga cagaagctgg ttaatgatgg cattcgctac
    13081 tggaaaagaa agataagaaa cctgggacaa gcttcgggtt atctgcatac actggcttgt
    13141 gaaggtggtt tgcctatccg catgattgaa aatgagagcg gttatttgat tacctatttc
    13201 agaaggatat atcaggcgct tcgtgggcaa tcatctcaat atcctgccgc taaaattgca
    13261 caagaattag gcgatacgat tcctgtcaca atgcaaaacg aactggtcta tgaaatagcc
    13321 ggtgaattct gcgagactct ctgccgatta cttagtgagc atcctcctca tagcagtgat
    13381 cccgtttctg cattacgtaa gctctctcca gactggcatc ttcaacttcc actcgttctc
    13441 cccgaagcga atgctgcaga gattgtaagg cgattacttt ctcaatcttc tgaaatacgc
    13501 agtgcaagta gtcttcaggt tgaaagaatc tgggtcgatg ttgatgacag ttggtattgc
    13561 gatgcccggt tccgattccc ggccacaatg cgtacagaac agttaacctc tttgtttgaa
    13621 tgccatattc agccggagca gacccgactc atcatttcag gaaaatggaa aaatggcggg
    13681 gcaaggttgg caatgctaag ccgttatgag cagcaagatt ggcgggtcga gttattacct
    13741 attgcgatgc aaaaactctc tggtgcagat gcaatggccg aaatctcatt gtcgcttcat
    13801 gaaggtccaa ttctgttagg tcacacaatt ccaaaaggcg gttatgaact tactgaagaa
    13861 cttccctggg tttttgaagc gatgaatgag agtgaatcgc agctaaaact tgttggtatg
    13921 gggtctgtga gttccaggct aaatgctctg tttatttcgc taccgaaaaa tagccattta
    13981 gatattagtg gagaaggtga gtttgatatc ccaaggttgc tgaaaaacag tgaacgcagc
    14041 ttaactaaaa taagtggagt atttagcgtt gttttacatg atggtgctgt ttgtacaatt
    14101 cgcacccagc aactttatga ttctgcgatt gaatattata ttaaatcgac agaagttgaa
    14161 ctggttaaat cggattatcc tgtccatcga gcctggccga agatcggttg gaaaaaggat
    14221 ctgcaatatg gcattgtacc ggagaaagaa cttttttggc gttccattcg ttcaggtaat
    14281 aatgcctggt attctgtcgc atcagaaatg ccaaaaggac agatagaagt ccggcgaata
    14341 gttaatgatg aggttttatt tagcggtaaa gttgtagttt taccagcaga cttcgatatt
    14401 aatattatac ctgagagtgc tcagcaaggc attattatgc tttctggtat aacggatact
    14461 agaattgata aatattcaaa caatgaaaaa gtaacactta agtccgatta ttcacaaaac
    14521 gagtgtgcta tttattataa ttcatcgctg atgctggaaa ataccgttga tttacgggtc
    14581 tcctggaaag atggttctaa tcttaaacta ttattaccta agccggttag cggtggccga
    14641 tttgtaacta atgatggttc tgttcatttt gatggtgtgg catctatagc acatttgcat
    14701 ggaatagatg ctgagttatt aaccatatca tgtgctggaa gaggatatct taatatcgag
    14761 ttattggatg aaaatccagt agctgaaaaa ttccgctatt tacatgccga ccttcctctt
    14821 ttatcaggac gcaatgacaa attacaacaa atttcacttt atgagaacta taatttgcta
    14881 aatgctatgc tggcatgtgc atggaacagc aatagtacat tatgtgttga tttttactct
    14941 gacagatttg gaaaggataa agcaacactc aatattaaac gctacgatgg tagttttatt
    15001 gaacacgatc aagggttact ggttgatata aaaaattctg ttgtttttcc tgcaaataga
    15061 atagacgaac tggttgttga tgctatttct cttaagaatc ctggcttaca catatcattg
    15121 ttaaaaaaag atgagtttgc ttatgatctt tcagctctga atgttcagga tagtccatgg
    15181 ttaattgtgg gaaaacttga tggtacagct cgcattgcac cagtaattaa atggatgcta
    15241 cctgtattgc agacaaatga tttattacta aatgctctat gcgaagcaga cccagaacag
    15301 cgcaaaaaaa attttaatga gctaattttt gaaatagata acaacccatt gcaaaattat
    15361 tgctgtttat taacagagta tattaagaaa tacaaaatga ataatggctt atccttgctg
    15421 gatctggact tgttcagagg tatttcgagt aattaccgcg tggttgttca attgttaata
    15481 tcatcatgtc tttctggtga tagcgatacg atttatgata tacaggaaga attacccttt
    15541 tcatggggat ggattcccgt ttcaatctgg aaagatgttt tccaaaaatg ttggacttat
    15601 ctggagaaac agattaacga taaaacatta gcattacata tattgcaacc ctttattgct
    15661 tttatgaacc atcgtgcaca tatcgatcgt cggctggctc cgattgcgaa tatgttactt
    15721 acatatagtg agagcctacc aaccggttgt gatgtattgc caactgttag tcgtgagcag
    15781 tttaatgaag ctaaacagat gctattaagg aaccccgaca gctttgggcg tatcagtatc
    15841 ttccctaaag aactttggtc tagtgctatt actccagagt taaaatctgt ttttaataag
    15901 ctttggattg aagataaata tcactcacgg cttgaaaaac gttttaattt gatgttagtc
    15961 gcagcgctgt taacccaaaa agataataac ctgatacatc aactgtctgc gctttttgaa
    16021 tttcactatc agcaagcccc acagcaatta ggggtaatct atcaatatta ttttgaacaa
    16081 gcaggagtat gtcattgatg gacgttcagc aactgcatta tgcgccactt ctaaatcagc
    16141 ttcatcagcg tgcggtgagg tcggtggtaa gtcagttggc attgcggagc aagtcgttga
    16201 ctcattatct ggaatcacaa tattttcagc ctcccggtca ggaggggtca ctgcttgcag
    16261 acccggtatt cgaaagtaca ttcggctgga tgcctgcgga tgaaacaatg gaggatttgc
    16321 gggacaagct gatctccagc ccgctggttg atgcattgag caaagcgcca gtgccatttc
    16381 gtcatcaact tactgcgtgg cgaacgattc tggaagacaa aaaatccttg ttagtgagca
    16441 gcggtacggg atccgggaaa acagaatgct ttatggtacc ggttctggaa gatcttattc
    16501 gtcagcaaaa atcgacccgc cagtcactaa cagggacaca agctattttc ttgtatccgt
    16561 taaatgcatt aattaacagc caacaggagc ggcttcgcga atggacgcgc ggatttaagg
    16621 ggaaaatacg cttcgcgcta tataatggcg aaacccggca taccaaatac gaaattcagg
    16681 aagatcagtt gaaagtacct gaacaagctt tgagtcggga agccatttat gaagcaccac
    16741 cttccatcat ggtgacgaat accaccatgc tggaatatat gctgattcgt cgtaaagatg
    16801 ccccaatcat cgaaaagtct caggggatgt taaagtatat cgtcctggat gaagcccata
    16861 gttatattgg ttctcaggct gctgagttag cgctgctttt acgtcgtgtt atgcaggctt
    16921 tcaatgttgg acccggcaca gataaacctg tgcagattat cgcgacctct gccactattg
    16981 gtgaagactc tcctgaaggt aataaggagc ttgctaaatt tgtcgctgat ctcgcgggcg
    17041 ttacagaacg tgatgtcaaa gtggttcgcg gttatcgaca aataccgcgg gtatcggagt
    17101 cgctgattcg tcatgaatac cccacatctt taacttctct taaatccctg agccctcagg
    17161 atctttacca acagctttgt cattaccgtg tggcccagca attgcgtcag gcgttaactc
    17221 atcccggacg gcaggcagta cgtttgagtg agttgctcaa tgttgccaga agaacctggc
    17281 cagatattaa tcaccgtgag ctactgcaac tcctcgatct aatggcaaga tcgcgagaag
    17341 gtgaactggc ttttacgcca ttacgtatgc atgggtttat cagaacactg gcgggtctat
    17401 gggcatgttc aaacaaacaa tgctcacata aagcacatga attaaatcag agtgactggc
    17461 catttggtca ggtatggttt gagcagcgcc aatattgtga ctgtggagcg ccggtatttg
    17521 aagttttacg ttgctccggg tgtggttcag cttatttgtc tgccaaagag gagatgcgcg
    17581 gtgatggaac gaactggttg gtagcgcaac cagccgctgc cgaagtggat gagtttgctc
    17641 ttgatgtaga tgtttattct gaagatgaag acaacgacga attagacaat aactctcaat
    17701 ttgatcgtct gattgcgagc gatggtgaaa aatatatcat ttcactggaa gaaacgacgg
    17761 cggggaaaat tgatagtgaa gcgcaaaagc actatgaaat aaacctgtta agaccggaat
    17821 ctcgaggtga gggacgcaat aactcctttg cttgtgtttg ctgtggtgac actcaacgga
    17881 aaaataatcc tttattccgt ccgcttcggc tcggcgcgcc ctttttttta aatgaaatta
    17941 ttcctactct gctggagttt tctccattac cgcaaacaag agaagagcag ttaggaccgt
    18001 ttaatggtcg acgcttgctc acctttacgg attcgcgtca gggtaccgct cgcatttcag
    18061 cccgcttaca gcaggatgcc gataaagcga cgttgcgagc tctttgttac caggagctgg
    18121 caaatattac agagccttca gcaaaactca gcccggcaca ctgtgcactg ttagagaaag
    18181 ttattagcca gttttctgtc ctccctttaa aaaattccat cgacgcgctg aaaaaagcga
    18241 tggctgcgaa tgaggagctt agcccgacgg acattcaggc acttaaaatt attgagccga
    18301 tggtgagttc cttaagcacg gagcatgctg aagcgatgca aatacttcag gctaactctg
    18361 gcaatcagca gaagaaaacc atgtcatggc aaacgctgtc tgaacagctg gttggcagtg
    18421 tggaccttgc cgatagaatg agaggcagtt ttaaagaatt gtctgggctt gatcttacta
    18481 aacaacagtt tgcagatttt tgtttataca gcgagtttgg tcgccgccct aaaaatgcct
    18541 ggaccctcga aagtctgggt ctggtagcca ttcactatcc attcattgat aaagttacaa
    18601 cctgcccgca ggactggaag acattattac ccgatcccga taagcaatta gcggaatggc
    18661 gtaatttatt gaagattact cttgatttct ttatccgtga aaacagtgcc gttttttacg
    18721 aaaacgagca ataccctcgc tggatggggg cacgtttccc ggtaaaaatg ctccaggggc
    18781 cagataaaaa aaacaaaggg cagaaacgtg atcaactttg gccacaaata cgcgatcgcc
    18841 attataaccg ggtcattaag ctattaatcg cagtctttcc ggcaatacaa cctgagcagg
    18901 ctcactggaa atcgttagtt aaccacttaa tgattgaggt ctgggatgca attcgaccct
    18961 gcttacgtca gtttgagtcg ggttatcagc tggatattaa acaacaagct gaattttaca
    19021 gtccaaagca agtgtggcgt tgcccctata ctcgacgtgc gttagacgtc acgttgctgg
    19081 gatactctcc gtatttgccg ggtagcaaag aaattgcacc tgaaaaagca gtgttgattg
    19141 aaatgcctga gcttcctgta cgtcactggc gtttgtctgg tggtggagaa atcgcccgag
    19201 aagaacgtct ggagtggctg gaaagtaatg cgctgattca acatgcccgc gaggaaggat
    19261 tatggtctac tcgcagcgac cgactggccc tgaaagatag ctggtatcgt ctggaggaac
    19321 actctgcgca gagaacgcct gagcagaacc agtttaatga aaaacaattt aagtcaggca
    19381 aagttaatgt gctgaactgt tccacgacca tggagatggg ggtggatatt ggtggtatga
    19441 gtttggtggc aatgaacaac gttcctccag cgccggcaaa ttatttgcaa cgtgcaggtc
    19501 gcgcggggcg tcgtggagag agtgcatctg ctgccattac gttatgtaaa aacacggcgc
    19561 atggaatgga ggtattcaaa gaccccctct gggcgttcaa cactactgcc agtgcgccac
    19621 gggttcgttt tgggagcagt agtattgttc aacgacatgt taatgccctg gttttaggtc
    19681 tgtttttacg agctgaagtt cctgatgcga caaaactgag ttgcaaatgg ttctttgaag
    19741 gtgatgagag ccagtgtctt cgttttctcc attggttaaa tcaccaggcc gatcagctgg
    19801 cggataaact taaacgcctt actcagggaa ccgtactgat gtcattgacg gcaacccagc
    19861 tcttgacccg cacacaagca atgatgcagc aggttgatat ccgctggaga agccaacttg
    19921 cgatactgtt ggaaaatatc gaagcgttga aggcggataa cagcgcgtgg gaagaaacac
    19981 cagcaggtaa agccatcgct tatcagcttc gtgattatcg cggtgcatat ctgttttcta
    20041 aactcattag tgaagcgttc ctgccggggc atggtttccc ggttggcgta gtcaatttca
    20101 attatctgac ggcagatgag ctggaaaaac gtcgcgcaat aaaagccact caggctgacc
    20161 ctaatgaagg gggagaaagc ttttctcgtc gtattgaaaa attgcccagc cgtgatttgc
    20221 ctacagcatt acgcgagtat gcgcctggtg cagatgtggt attgggcgga aaagtatatc
    20281 gttcctcggg cattatgctt ggcaaggtgc ttgccagcgg gcaggagctg tcaggcgatc
    20341 atcatattcc gtggttctgg cattgccgta aatgtggtgc gggggcaacc agtactaccc
    20401 atcctgtcga atgtagtcac tgcaaagcgg atatacagca acttgatgtg aagcgttatt
    20461 tacagcctgt tggttttgcg acagatattc gttatcaggc acataatgat gtatccatgc
    20521 ctgcacaact tccctggaaa gatccacgtg ttcttgtacc gtcttcggtg tgggtttctt
    20581 tacccgatgc tgggctagga cgttaccgtt ttagccatag tggggaattg ttccacttca
    20641 gtgaagggga atttgggcac ggctacgcaa tttgcctctc atgtggtcgg gcagaatctc
    20701 aaactcaacc ccagcgtacc cctgaaaatc taaagaaccc agagagggaa aatacgcatt
    20761 acctgttgcg agggggcagt aacgatcgtc aggggagcaa taaattgtgc cacggtcatg
    20821 tgcataaaga tctgtggttg ggatatagca gtcgtacaga tatggttgag ctgcaactca
    20881 atgatgataa tggcctgttg atccgtgatg aggttgccgc gcgttccctt gcggtcgctt
    20941 tgcgtgaagg gttagcgcat aaattgggta tcgaaaatac tgagctgggc gtgacgacgc
    21001 agcaagcgcg cgatatcaat ggctataccg gatattccat cttcatttac gacaataatg
    21061 cgggtggtgc aggttatgca gtgcaattga tcgaccattg ggctgatgtc tttaattacg
    21121 ccagaaaatt gctcgattgc agttgcgata aattctgtca ccactgcttg ctcagctacg
    21181 acagtcagca ttatgtcaac agattagatc gtcaccatgc attaaccctg cttaccaatg
    21241 tacggttgca gcgacttaac ctcgcgccag aatatcagta cttcggcgat ggtagccggg
    21301 ttgaaactaa ccctctgagt ttacgtattg cgcaatgcct gaacagcgag atttatgact
    21361 cctgctcgct ggttcttgcg gggccgcagg aacagtggga ctttgcccaa tggccattat
    21421 ttaaagagtt gctccagttt gcctcctccg gtgggaatgt tgagttgctg gtggccacgc
    21481 cgctggcaaa cttaactgat agctccagac atcagctttc cgcgttggcg gcgatgccgg
    21541 gaggtcgctt acaagtgaaa tccattgcca ctgcgcagct catgcaagga aaggggcgct
    21601 ggcttgcgca ggtgactcgc gaagggcaaa gccaacagtg ggccgcagac gacagcgcta
    21661 ccgttgcacc tggtgaatta tggggacaaa gtgcgtcttc accagtagtg acacttaaag
    21721 gaacgtccgg aaaaaccttc tcgggtcaga ccttaagtgc agaagattta ctgcccgcct
    21781 tacccaccgg ggcggtgcgc atcaatctct gtgaacaact ggacgggccg ctggaaggtt
    21841 ttggttcgcg tttctggtcg ttagtcacgc aacagcacgc tggctggaag caggcattta
    21901 cccgccacaa agagataacg catgttgagt acagcgatcg ttatcttaat agcccattca
    21961 cggcgcgttt gttaggtgaa atcttaaccg agctggttga acagggaatg gcggaacgcg
    22021 ccagcctcac tgtttgcgtc aaaaaattgg attacaactc tcgccaacac gacgctctct
    22081 ataacgcgtg gcttaatgag gaagatcgtc agcaggttgt gaccacttta cttgaagaag
    22141 gatatctggg gcctgcgtgg ccgggagcaa ttagctggtt aacgggggat aaccagagta
    22201 cggagcatgg acgagaactg acagtcacct tcagcgatgg cagtcagcat tatgtcctgc
    22261 tcgacatggg gcttagctac tggcgctgta ttgaagatac cttctttgat tttgccctgc
    22321 gagtaccgca gcaagttgaa cggctggcaa acacgcgagc gcgagctgtt gccccgggta
    22381 atgaccttag gagttatatt atcgctggat aaataattgc tcccctgggg atatcttcca
    22441 ggggagttga cccagctcac tttttaataa tttttgtttg aaaatcatgc gcaatttatg
    22501 cgcattccct cccattttgt cactttgccg ggggccttca tgatatcttt aaggccctct
    22561 tttttatcgc agtgtgccgc gccacatttt tcaggatgac gttaccgcca ctgccccaaa
    22621 tagacagaaa aaccgatgaa taagcaaaat tatgcacctg gtatgcgggt ggttattcgt
    22681 gatgccgaat ggcgtattcg ccgggcggat gacagtggtg atggtgggta tttgctgacc
    22741 tgcgatggta tttcagagct tgtgcgcggt aaagaagggt tatttctgac caaactggaa
    22801 caaaaagtag agatcctgga tcccgcaaaa acgcatttgg tggaagatga atccgccaat
    22861 tatcaagcgg cacagttgta tattgaaagt cagttacgcc aacgcgtacc gacagacagt
    22921 aaagtccatt ttggtcatct ggcggcgatg gattccatgc cgtttcagct cgatcctacg
    22981 cggatggcgc tggcacaacc gcgtcagcgg atactcattg ccgacgctgt tggtctgggt
    23041 aaaacgctgg aagccggtat tctggtttcg gaactgatcc gtcgtgggcg cggaaaacgt
    23101 attctggttc tggcggttaa atcgatgctg acacaattcc agaaggagtt ctggagccgt
    23161 tttgcgatcc cattgacccg cctggattct gctggtttac aaaaggtgcg taaccgcatc
    23221 ccaaccaacc acaacccgtt ccactatttt gacaagacca ttatctccat cgatacgctg
    23281 aaacaagaca ttgaatatcg tcaccatctg gaaaatgcct ggtgggatat tattgtcatt
    23341 gatgaggccc ataacgtcgc agaacgtgga accagttcgt tacgcagtaa actggcaaaa
    23401 ctgctcgccg ggcgcagcga tacgctgatt atgctttcag caacaccgca tgatggtaag
    23461 gctgaaagtt ttgccagcct gatgaacatg cttgacccga cggcgatcgc caacccgaaa
    23521 gagtatgaat acgccgattt tgccgataaa aatctggttg tccgtcgctt caaaaaagac
    23581 gtgaaagatc agatgtccgg cgagttcccc gagcgcaata ttgtcaaatt gactcgcctg
    23641 gcatccggtg ccgaagaaga agcataccgt cgtctggttg agagccagtt ccgtgatgat
    23701 gacgatgaac aggcccagtc taacaaaggt cgtctgttca agataaccct cgaaaaagcg
    23761 ctattttcca gcccgatggc gtgtgccagc gtcgtggcga atcgtctgaa gcgtcttgag
    23821 agccgtaaag atcataatag ccagagccag atcaacgaac tggagtcatt gctgttggcg
    23881 ctgaataaca ttgatgccag ccagtttagc aaataccagt tgctgctcga caccatccga
    23941 aaagacctcg cctggaaagc caataacacg gaagatcgcc tggtgatctt caccgaaagt
    24001 attaaaacgc tggagttcct cgaacagcaa ctgcgagcgg atctgaaatt gaaagatgac
    24061 cagatcgcca cgctgcgcgg cgatcagggc gatacggtat tgatggaaac cgtagaagcc
    24121 tttggtaaaa cgcagtcgcc gttgcgtctt ctggtttgtt cggatgtggc atctgaaggg
    24181 atcaacctgc atcaccttag ccacaaaatg attcactttg atattccgtg gtcgttgatg
    24241 gtattccagc aacgtaacgg gcgtattgac cgctatgggc aaaaacatca gccccaaatc
    24301 cgctatctgc ttaccgaggc cagcgaacca cagatcaatg gcgatatgcg cgtactggaa
    24361 gtgttgatca acaaagacga acaggcgcag aagaacattg gggacagttc ggagtttacc
    24421 ggcaaattta cccaggaaga ggaagaagag caggttgcag agttcatgat gcaggatgat
    24481 ggtgccagcc tgtttgatca actgctgaac agcaacgtct cggaaagcgc cgaacacgat
    24541 ctgtttggcg aaatatgcag tgcggtttcc agcgatgcct caatggtcac cgaaacagac
    24601 accagcttgt ttgccagcga acaggcgtac tgcgaaagag cattgggtta cctgaaagcc
    24661 agtggtcaaa ctatccagta tgaaaccttg cctgataata ccttgtcgct ggtggcaccg
    24721 gaggagttac gccgccgctt caaccagcta ccgcctgaaa ttgccccgga gaactggcag
    24781 ctctatttaa gtcaggataa aaccgtcatc accgacgcga ttgcgcgcgc tcgcggtgag
    24841 caacatgcct ggcccgatgt gcagtatctc tggcaaatca acccggtagt gcagtggctg
    24901 gacgataaaa tctcttcggc ttttggtcgt catcaggctc cggttatccg gctgccatac
    24961 ctgcttgaac ccgatgaaga tcacttcatt ctttccgggt tattcccgaa ccgtaaatca
    25021 catccgatgg tgaacccgtg gatagtggtg agctttaacc gtgaatcgct aatcggcagt
    25081 cagcctttcg ctgagttttt acaacgtcat ccgcagttga gtaacaagct gactaacagc
    25141 ggcggtaaag atcgtaatca ccaacgccag caggatttac tggaagcggc tattgctcat
    25201 gcccgggagg tattcatcca tgatcggaat gcgtttgaaa cgcacattaa ccagcaactg
    25261 aatgagcatc tgcaaaagtt ggacgttttg cgtgggcggc agttgagcca acttgagctg
    25321 gattttgccg ataacaaaca gcaattgtca gtcaagcaga gccgtaaaga gcagagacaa
    25381 cgcgaaatcg aacacaattt tgacagctat atcgaatgga ttgaggacac catgacgact
    25441 gaaaaagaac cctacattca ggtaattgct gttatcaccg gagcggaggg ttaatcatgg
    25501 cgctggtcgg tattaataac gaaaacgaat tttactctaa ccactatttg ggtgaggttt
    25561 tcaccagtga tatccgcgat gtgctggaac cctggatagc ccaggaaaat gcagcgcgtg
    25621 aagcggagcg tgccgctcgt gaacagggca aagacgtgga gccgggatac cgcgctccgt
    25681 ggaaccagtt taacagtctg gcgactgagt ttttccgcaa acttgccgag cacgaaaaac
    25741 agcgtcagat cccgcagcgt ctggccgatc aacgtaatcg ctggcagcca ttgttaaagg
    25801 cgctgggcta cgaaattacg ccacagatcc aaatgctgga tgacgataca ccgctgccgg
    25861 tactggcgcg ttacaacagc actgacggta gcccgtggct gtggattgtt gaagcacacg
    25921 atcaggaaga aggaacgctg gatccgctgg cgctctcctt actgaccgcg caattcccgg
    25981 cggataccga caaacataag cacgacagcc tgcgcaaaaa agccaacggt gaatatcgca
    26041 gctggcagga tttgctctct acggcggtct tcacccaaaa tgaaccgccg cgttttgtgc
    26101 tgctgctcgg taaccgtcag ctattgttgt tggaccgtac taagtgggcg caaaaccgtc
    26161 tgctacgttt tgattttgaa gagattttaa gccgtcgtga aacggatacg ctgaaagcga
    26221 ctgcggtgtt gttacataaa gattctctgc tgccgggcag tggggcacct tatcttgact
    26281 cgctggatga caattcgcac aaacatgcgt ttggtgtttc ggaagatctg aaatatgccc
    26341 tgcgcgaaag cattgagttg ctgggcaacg aagcgatgca ttatctgatc gaccgtggcc
    26401 tggcaaacta tacaggtaat cgtgcggtgg acccggatga actgagccgc gaatgtctgc
    26461 gttacatgta ccgcctgctg ttcctgttct acattgaagc gcgcccggag ctgggttatg
    26521 cgccaatgac cgccaaaacc tatctgcaag gttacagcct ggaaacgttg cgcgatctgg
    26581 agatgatccc gctgaccagc gaagaagatc gcaacgggcg ctacttccac gacagcctga
    26641 atatgctgtt taaactggtg cgcgaaggct acaacggcgg cgtgaaaatg cagagtgacc
    26701 tggagagcgg cgaccggatc accatccata gtcatcagtt cagcgtcccg cgtctggaaa
    26761 gtcatctgtt tgatgccaac aacacgcgca ttcttaaccg cgtggtattc cgtaacgaaa
    26821 ccctgcaaca gattatccag gcgatgtcgt taagccgccc ggccaaaggg cgctttaacc
    26881 gccgcggacg tatttcttat cgccagttgg gtatcaacca gttgggtgcg gtgtatgagg
    26941 cgctgctctc ctatcgcgga ttcttcgcca gcgaagatct ctacgaggtg aagaaagccg
    27001 gggaagagtt taacgagctg gagacgggtt acttcgtcag taaggatgag attagcaaat
    27061 accacgaaga cgagaaggtc tacgagaaag acggcagtct gcgcattcac cgcaaaggca
    27121 gctttatcta ccgtatggcc gggcgcgacc gtgagaaatc tgcctcttat tacaccccgg
    27181 aagtgctgac ccgctcactg gttaaatatg ccctgaaaga actgtttaag gagcagattg
    27241 atcccattag cgatccgcac gccaaagctg atgccatctt aaacctcacc gtgtgcgaac
    27301 cggcgatggg cagcgcggcg ttccttaacg aagccatcaa ccagctggcg gaagcgtatc
    27361 tgttccacaa gcagcaggcg gaaggtcgcc gtattccgca ggatcgttac acccaggagt
    27421 tacagcgggt gaaaatgtac attgccgaca acaacgtttt cggcgtggac ttaaacccgg
    27481 tggcggtgga actggcggaa gtgtcgctgt ggctgaacgc cattagtggc gatgcgtttg
    27541 taccgtggtt tggttaccag ctgcactgcg gtaactcgct ggtgggcgcg cgccgtcagg
    27601 tgttcaacaa gagcgaactg acctacaaaa aagccaaaga tccgagctgg cttaacagcg
    27661 agccggtcga actggcgatg aacacgccgc gtgaagagac gcagattttc cacttcctgc
    27721 tgcccgacgg cggtatggct aactacagcg ataaaactgt taagcagcgt tatccggatg
    27781 acttcaaagc gctggacagc tggcgcaaag agtttattaa aagctttgcc gggcatgaga
    27841 ttgctgatgt gcagcgtatc agcgaaaagg tggaagcact gtggaacacc tatcgccagc
    27901 aacttaaagc agaacgtctg aaaaccgccg acagctaccc ggtgtggccg gcagaaaaca
    27961 gcgagcagac gcgttcttcg ctgagcagta aagatgaaac ctttagcggt cgtcttgaag
    28021 ataacagcgc ctaccagaag ctgcgttggg taatggacta ctggtgcgcg ctgtggttct
    28081 ggccgatcga caaagccgat gagctaccgg atcgcggcac ctggttgttt gagattgaaa
    28141 ccctgcttga cgggattgta atcacggaaa aagtcactga agttgcggag cacaccaccg
    28201 gcgatctgtt tgccgaagaa ggcctgctgc gagaagagtc ttcgctgttt tctgttgctg
    28261 gtcgtctgaa aaccgaggtg ttgttccgtc atttgccgcg tctggcgatt gtcgatgccc
    28321 tgagaaagca gcaccgtttc ttccactggg atctggagtt ctgcgacctg tttgccgagc
    28381 gcggcggttt tgacctgatg ctcggaaacc cgccgtggct gaaagtggaa tggcaggaag
    28441 cgggcgtgct gggtgattac gagccggaat ttgtgctgcg taagctgagc gcctcgaagc
    28501 tggcaacgtt gcgtattgat acctttaacc agatcccggc gctggaagcg gcctggcgca
    28561 gcgagtatga aggctgtgaa gggatgcaaa acttcctgaa tgcgcagcag aactacccgg
    28621 tactgcgcgg ggtgcagacc aacttgtata aatgctttct gccgcaggcc tggcgattag
    28681 gggcgcagaa aggcgtggca ggtttcctgc acccggaagg gatttatgat gacccgaaag
    28741 gcgggcaatt acgtgcggcg gtatatccga ggctgagggc gcattttcag tttcagaatg
    28801 agttaaattt gtttgttgaa gttgatcacc atgcgaagtt tagcagcaat atttattctg
    28861 ctagccctag cacagtggga tttgaacata tatctaattt gtatgctccg caaactattg
    28921 atgcatgttt tgaacattct ggcagtgggg acattcccgg tctcaaagac gagattgaga
    28981 gcgagggaaa attaaaagtt gtatggaaca catctggcca ccgttctcga ttaataagta
    29041 tcgccactca tgagctagaa ttatttgctc gtctatatga cagcgaagga acgccagcct
    29101 ggcaggcacg tttgccagcc ttacatgcta aacaacttgt tgctgtactg gaaaagtttg
    29161 ctaatcagcc gaatagatta ggtgatttgc aggggcagta tttttcaacg gttatgttcg
    29221 atgaaacata tgctcagagg gatgggacaa ttttacggca gactcaattc cctcaagatt
    29281 catcacaatg ggtactgtct ggccctcatt tctttgttgg gacgccgttc tacaagactc
    29341 cgcgcgaaaa ctgtacgctt aacagcgatt atgactgcct ggacttgcta actctgcctg
    29401 acgactatct gccgcgcact aactacattc cggcatgtga tgcacaggag tatgcaaaac
    29461 gtactccatg cgttacatgg actgaactgg ctgaagatga accgaagaag gtaacagatt
    29521 attatcgctt agctatcaga gccatgttgg ctcaatcggg ggaacgtaca ctaattagtg
    29581 ctatttatcc gccagaaata agtcacatga acgcagtacg ttcttactgc tatagctcac
    29641 agaatctgtt actcgaacat tcaggtatgt gtttttcttt accttttgat tttatttgta
    29701 aatctactgg caaggcaaac ttacatcaga tgcttgatgg tttctcatac gtattattca
    29761 atccgagaca aaaggcatta ttatactgct tagtattatc attaaattct gtaaatgatg
    29821 tatatgctgg cctttggcaa tcctgctaca ccccagactt caacacccag cgttggagcc
    29881 gcgatctccc gcagctcccc caggatttct tcgccaaact gaccccagag tggcagcgta
    29941 actgcgcttt acgctctgac tacagtcgtc gtcaggcgct ggtggaaatc gacgtattgg
    30001 tggcgcaggc gctggggtta actctcgaag agctgcttac catttatcgc gttcagttcc
    30061 cggtgatgcg ccagtacgaa gcggatacct ggtacgatca aaacggtcgc attatcttta
    30121 ccccaagcaa agggctggtg ggcgttggct tgcctcgcac cgcgcgtaaa gctgacctga
    30181 aaaacggctt tgtctttaac gtcgacagcc cggagtggac cggcggtgac tgcaccgatc
    30241 aagctatcgg ttgggatgat gtcaaacatc ttaaaaccgg taccgtcagc gtcacctttg
    30301 atgattatac ccgcagcgac gaaggtgagc gccgtaccgt cacctggcag gctccgttta
    30361 tcaagccaga tcgcgaagat gactacaaag tggcctgggc gttctttgca caagataagg
    30421 agagcgcctg atgttgccgt ccgttgtcag tcggcaggtc gccgacagcg ttgccgcctt
    30481 tttacgggcg gcgttcccgc taaacagccc gctgtttaac ggtgagaata acgataatgt
    30541 ctcaatgctg gagcaatttc tggcccagcc ggaaacgctg ctgaaaggac catacctttc
    30601 cgcccagttg ccgtttcgta aaagtgactt accgctgaat tttttcccta atctgacgct
    30661 gccgttcccg ccgcacgcgc atcaggcccg ggcatttcag cgtctgggaa gcgacgcgcc
    30721 acagccgacg ctggtcgcaa ccggtaccgg ctccggtaaa acagaatgtt ttatgttccc
    30781 gctgctcaac cactgcgcgg gcgcgtcaca agccggggtt aaagcgatta tcatctaccc
    30841 gatgaacgcc ctggcaaccg atcaggccag ccgtttcgcc aaaaccatcg ccagcgatcc
    30901 acaactgcat ggcaaggtga ccgtcggcct gtttgtcggc gacagcgaaa tcgaacccag
    30961 caaaaaaatg tcggcagaaa aggtcattac ctgtaagcac acgctgcgcg aaagcccgcc
    31021 ggacatcctg ttgaccaact acaaaatgct cgactacctg ctgatgcgcc ctggcgatca
    31081 gccgctctgg cgttacaacc agcccggttc attgcgttac ctggtcgtgg atgaattgca
    31141 tacgtttgat ggcgcgcaag ggtcggatct ggcctgtctg gtgcgacgtt tgaagcatca
    31201 cataggtgtg gataaccaac gattcgcctg cgtcgggaca tcagctaccg tcggcgacga
    31261 actggggcaa ctgctcgatt acgcaaaaac aatctttgac cagccgttta ccgatgacgc
    31321 cgttattcgc gaagatcgct acacggcagc ggagtacttg cagaattacg ccattgcgta
    31381 cagtcagtat ccggggccgg aggtcgcttc ggccctggac ccgcagagtt acgctacccc
    31441 ggtagcgtat cttaacgggc aaatcccttt gtggttcccc gacagcgact tgcaactccc
    31501 ggacgatctg gagagcgacg acgggcgtga aaagcgtatt gcgctggggt cactgttacg
    31561 ccgccacagc gtgatgcatg tcttactcca tgatctgcaa ggcgggatcc tctctgaacg
    31621 agagtgtctg gaaaatctcc aggtcatgct ggcagaaagc gccgaccatg cccgacgcgt
    31681 gctgcaaagc attctggcac tgatcgcgca ggcacgtctc gaagtaccgg aaacggaaca
    31741 cgatcggcaa aaacgtctcc aggcgaagaa agcgcgtccg gtgctgccgt ttgtgcaact
    31801 gcgctcccag ttgtggcttc gtgagatgcg tcgtctggtc gccagcgtgg cgaaaacgcc
    31861 ccgactggtg tttgccgatg atgtggcggt ggaagatcgc gaacactatt tgccggttat
    31921 ccactgccgt gactgccacg caactggctg ggccagcctg cgtcatgggc aaagcgttca
    31981 gttggaaaca gacctcgacg gcatttatcg ccagttcttt gaaaaagggc gctccatcgt
    32041 ccttgctttc ccggataaca acgaaaaagc ggtcagtggc gcacaccaga aactgtgtcc
    32101 tacctgtctg aaactgaacc gacagtccag cgtccagtgt ggacattgcg gtcatacgga
    32161 actgttgcag gtactgatcc cggatatgct caaagagcgt aaggacgagc tggagtttgc
    32221 taatgaatgc ccgtactgca acagcaaaca ggggatttcg attctggggt cacgggctgc
    32281 cagtctgtcg gcggtgatga ttcaccagct ttatggcagc cagttcaacg aacataaaaa
    32341 gctgattacg ttttctgact cggtacaaga cgccgcgcat cgtgccagct tctttactgc
    32401 ccgtacctgg ccgctgatga ttcgtgggca aatctccagt gttcttagcg aaaaagagac
    32461 attaccgctg gatgcgttcg cggcgcgtgt ttgccaacaa acgcgtgaac gttcaccgga
    32521 cgatgctcat tttgcagcaa acgtcatcgc accaaatatg cagtggctga atgattatca
    32581 gacgctgaaa aatgaaggca aactggcgga gaactccgat ctgccggagc tggtttccct
    32641 gcgcctcgac tgggaggtgt acagcgaatt taccctgcgc gcgcgtatcg gacgtacgct
    32701 ggaagccacc ggttatgccg ttgccggagt ggataaagat cgcttcaatc cggcaattac
    32761 cgccctgcat caacagcttt gtgaagagct gggtgatgag atgtctggtg tgagcgtcga
    32821 gcaggtagcg cacctggcgc tgggggttat ctggtatcaa cgccaggccg gggccgttat
    32881 gcatccggcg ttcgaaagtt atcgccgtga tggcacgacc tttatgatga cgcaaaaaga
    32941 gcgtacctcg cgctatatgc cttctatcgg tccgaaaacg cgtaaaccgg cttatgcgtc
    33001 gtttgaaaaa attaatggtt tccatcgcct gattggcgcg aagtctaacc ccagttggta
    33061 tcagcattgg atcaatcgca cgctgagtaa cggcaataat ctgtttatca gtagcgttgc
    33121 ggaaacagtg ttacgacgcc tgtttacggc gttgaaaata gccggagtag taaaagattt
    33181 tgacaccaaa gggcgtgaag cctggggact ggtgccttcg gcgctggtgg tgagccgtga
    33241 cgtcgttcag cttaattgct catgttgccg tgaggttgtc cgggctccgg ccgatcaagg
    33301 ctggcagtgg cgtaatgcgc cttgtctctc tttacgttgt gaaggtcgtt accaggaagt
    33361 cgacaatacg gcgacctggc gttgggagaa catggacatc gcgcgcgtac agggcgcaga
    33421 acacacgggg ctgcttagcc gtgaagatcg cgaagtcacc gaaaaatcgt tctaccgcgg
    33481 caaccaaccg tggaatatca acctgttgtc ggcaacaccg acgctggaga tgggtattga
    33541 cgttggggat ctttccaccg ttttactctg ttctgtccca cctgctcagg ccaactactt
    33601 gcagcgtatt ggtcgcgccg gtcgtaaaga tggtaacgcg ttaaacatta ccgtggcaga
    33661 agggaacccg cacgatcagt ttttctttga agaaccactg gagatgatgc agggccaggt
    33721 gcaggctcct ggcgtcttcc ttaatgcaac ggctattctc gaacgccagc tggcagcttt
    33781 ttgtatggat aactgggtga aaaccggtgt accagaatcc gctatctgta aaaacgtcaa
    33841 acagatgctg gatgagctgg agttcgggcg taaatcgggt tttccgtata acttactgcg
    33901 atatatcgaa cagcatcatg cggagattgc tgcgcaattt acggcaatct tccccgatct
    33961 tgccgcagaa actcgccaac agctgttgtc ttatcttcag ggcgcgccag gacaacgctc
    34021 gctggtgcag cgtatcgaag aagcgttaaa actgctggtg gaagatcgca aatctttgcg
    34081 ctcacgcatc gataaactca agcgcagtat cgataaactg gagaacgcgc cgcgcgatca
    34141 aaactttgat agcgatatgc gcgagttaac ttcggaacgg caggcgctga tggcgctggt
    34201 caatcaaatc aacaataagc aaacgctgaa tttcctcacg gatgaaggtc tgctgcctaa
    34261 ctacgccttc ccggaagccg gtattacgtt acgttctgtg ctctggcggc gtaaagaagg
    34321 cggggaagca cgtgaatatc aaaacaccac tttcgaatat gaacgcccgg catcaacggc
    34381 gctggctgag ctggctccgc tgaataattt ctatgccggt gggcataaag ttgaaattga
    34441 gcagatagac ctgaaggtct ctgagccgga gaactggcgt atctgtagcc actgtaacta
    34501 cagcgaaaat atcgaccaga cgggcgacca gcataaatat tgcccgaaat gtggcacacc
    34561 ggggtgggcc gatgccggac aaaaaaccac gctgctgaaa cttcgccagg tttatgcccg
    34621 cgccagcgca cgtgatagcc agatcagcga cgagagcgac agccgtgaac ctgctttctt
    34681 ccagcgtcag ctattggtta gtttcgaaaa agaagatgta tcggcggcat acgcgattga
    34741 ggaaggcgaa gttccgtttg gttttgaatt tttaagcaaa gtcaccctgc gggatatcaa
    34801 cttcggcaaa atggcggatg atgccaatga gttgatgatt gccggagaag cgaagaagcg
    34861 aaccgggttt aaagtttgcc tcgggtgcgg catggtgcag cgtcctcgcg atcatgaacc
    34921 acgtcatgat ttgagctgca aatatcgcac ggaaccggaa aaggcgaagt tcgaagatta
    34981 tctctacctc tatcgccagc tggagtcaga agcgttgcgt attctgctcc cggtaagcag
    35041 ttacagtaat gaccgtgtcg tggaggcttc tcttggggcg gcgatccagc tcggtcttaa
    35101 acattacttc aaggggaatg tcgatcacct gaaaggcgtt gtgtaccgcg agccggaaaa
    35161 tgaaggcgaa tcctggcggc agtatctggt gatttacgat accgtcccgg gggggactgg
    35221 ttctcttaaa gaactgatgc gcactcccga caatttgctg aaattacttg aactggccta
    35281 tcaggcaata gtggaatgca gttgcaacaa tgatacgcat aaagatggtt gttatcgttg
    35341 cgtttacgct taccgtgacc gtgggcgcat gaagtatgtc tcccgcgatc aggcgcgtct
    35401 cttgctggcg aaaattttgc aggcaagaga ttcgatacgg gtaattgatt ccatcaaaaa
    35461 catttccctc gaagccatga tgggcagtga gctggaaaaa cggtttatta gctgcctgca
    35521 agaaaataaa aacctgattg tcagtcgcag ttacgcgcat cagggcgctg ggtggattat
    35581 taacacccgc agcgaaccga tgatgagctg gcatcttaaa gctcaggtgg atttaggcgc
    35641 taaagaggcg gtggggatcc cttgtcgccc ggattacgtg ctatatccgc ttatgcagtc
    35701 tgatgccatt aaaccggtgg ctattttcct tgatggcttt gcgttccaca aaaatagcgt
    35761 cgcagaggat gtgcaaaaac gccaggcgat tcgggatagc ggtaatttct gggtgtggac
    35821 agtcacgtgg gccgatctca aagagccagg gcttaaacat gttcaggatg tcctgggact
    35881 tgggcataac ccggatatga aacagcctaa gttttataac ctgtttcatg ataccaactt
    35941 tgcggccctt gaagcgtcgt tccgggaacg caacagtttt gcactgctgc tggattatct
    36001 cgccgatccg ggcggaagaa cgcagttatg gcagagaatg gctgctgcac atgcctgggt
    36061 gtggttggat gtgaagaagt cacaggacgc gggaacgaaa caaaagtatg cctatgaaat
    36121 gcaggaaaac gcgccagctt ggcggctagc agagcttttg ccagacgaac cgtttgtctt
    36181 tggtggttta ctcgatagct gtaactcatc gcagcaattt attgagctgg cttcggtgtt
    36241 accacaacaa gccattaaac cgacgaccag cgtcgcacaa atgcgcagct ggctgcgttt
    36301 gcatatctgt tttgacgatc gctatacgca ggatgacggt tatgaagcgg gactcaatgg
    36361 cttctggcga ctggtaaacc tgttacagtt cctgccggat atgaccttta ccagccgtaa
    36421 agccgtgcat ttaccgcagg aaagcgttga cactaaagtt tcggcaacgc cagaggcagt
    36481 accagagact gatgagagtt gggctgagat tattgagttt ggcttattaa gcgctgaaga
    36541 gatcgcgttg cttcagtctc attctctctc tgcgccgact ctcggctacg aattgcaaaa
    36601 tgatgaaggt gaaattattg cggaagccga tctcgcctgg ccatcacaaa aatgggcatt
    36661 gattattgat aatcaagaat ttattccatt attcacaacc aggggttggc acgttgcatt
    36721 cgggccaatt gacgaaaata cattgcaaca tctgtccgga ggtgacaaat gacggtgcaa
    36781 acgcccaagg tggcactttc tgatggtttt ctgggcgcat tcgcgcggat cccaaaggca
    36841 cagcaaaaaa aggtgcagga attcatctcc aaattccgtc aggacccgac cagtaatgga
    36901 cttaactatg aaaagatcca cgatgcgcgc agtaaaaatg tccattcggt gcgaatcgat
    36961 caaacttatc gcggcattgt tctgaaacct gaacagggtg ccctgtatat gctgatgtgg
    37021 gtggataaac acgacgaagc atatgactgg gcgcggcggc atgattgttc aattcacccg
    37081 gttaccggcg cgattcaggt cattgatatt agctatatca aaccggcagc ggaaacagtg
    37141 gttgataaac cgaaactctt cgctgcttac agcgcggaac agattctggc tctgggtgtg
    37201 ccgcctgtct ttattgatca ggtgatggcg cttaccgatg aagccgggct taatcaactt
    37261 gaaagcataa tgccagcaga agcctgggaa cctctgcact ggctggcaga agggctggat
    37321 taccaggaag tgctggaaga gtttaacgat catcgtgatg agcctgttga taccaacgac
    37381 tttatggagg cgattgaacg aagtaaacgt cgcttccatg tcgttgaaaa tgaacaggag
    37441 ctgttgcaga tgcttaatgc gcctttagaa aaatggcgtg tatttctgca tccgagccag
    37501 cgaaaattgg tggagtcgcc cgctaatggt ccggtacgag tactgggcgg tgcaggtaca
    37561 gggaaaacgg ttgttgcgat gcaccgtgcg cggtggttat ctcagcgtct ggcagataaa
    37621 cccggtaaga aagtactttt cacgaccttt acgcgtaacc tggcggcaga tattcgcgct
    37681 aatctccagc ggttgtgtac gcgagaagag atggcgcgta ttgacgtcgt taacattgat
    37741 gcctggatga gcgaccaact aaaacgccac ggttatgatt tccgcgtggt atacgacagc
    37801 gatgaagggc gacgcaaatg ctggaactat gccttgcagc aggcacctgc cgaactgggg
    37861 ctacctgata atttctatgc tgaagagtgg caacgcgttg tccagccaaa tgcggtttac
    37921 acccgggagg aatacctcaa agtcagccgg gttggacgtg gtacagcgtt atcccgtatt
    37981 caacgggcga aaatctggcc agtctttgaa gaattcagag cacaaatggc gcgtgccaaa
    38041 ctgcgcgaaa tgggcgatgc catgcatgag gctattgttc tgtttaaaga aaagcaggtc
    38101 caactacctt atagcagtat tgtggtcgat gaagctcaag atatcggtgc cccggcattt
    38161 actcttattc gcagcctggt tcccgagtcg cccaacgatc tgtttattgt cggggatggg
    38221 catcagcgta tttatcgcaa taaggtcgtt cttggtcagt gtggtattaa cgtccgtggt
    38281 cgtcgcagta aaaaattgaa gattaactat cggaccacag aagagacgcg tcaatttgct
    38341 gttgggctgt taactggtgt gaaggttgat aacctcgatg gcgaagcgga taccagtaat
    38401 gattatttgt cattactgca cggggaaaag cccatgatta cgcatgcggc agatttcaaa
    38461 gaagaggctg caacgctcgt caagcaaatt caggcattgc tggcgaatca ggttcgttct
    38521 caggatatct gcattactgc gcgtactaaa catgcttgtg accgctacgc atcagcactg
    38581 aatgacgccg gaattgagac atttaattta ggcaatgata gcggtgacag cgatgcacgc
    38641 cctggcgtgc gggttgcaac gatgcaccgt attaaagggc ttgagttcca gtatgtgttc
    38701 ctggtgggga ttaacgaagg cgttgtgccg gaaataaaag cattagcatc cgatgatccc
    38761 gttgaacagc gtgatgcatt atttaatgag cgcgcgttac tgcatgtggc tgcaacccgt
    38821 gccgtaaaag ggctgtttgt ttcatcatct ggtgtcccta gtccgctatt agtggctgac
    38881 tgataatatg tccaaaacag gccaggagcc gaaacgctcc tggtctatat aaccgttctg
    38941 gcaactggcg atatttatat tgcttagctc ccaacagcca gacaatatca acttagaaat
    39001 tatattcgct tatgcggttt gcatattgca cagtacaacc tgttttctct atatctaacg
    39061 taacgtcagg atgtggatga tttttggatg gggtgacaca ctggacaaat atagctggca
    39121 cgaaaaggct ggcatttcca gctgaccaat agtctctgga accatgatga tgataa
//
LOCUS       DAZKIF010000028        38116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZKIF010000028 DAZKIF010000000
VERSION     DAZKIF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.028"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            122..733
                     /gene="ruvA"
                     /locus_tag="ACHOTW_001510"
     CDS             122..733
                     /gene="ruvA"
                     /locus_tag="ACHOTW_001510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9843641.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            742..1752
                     /gene="ruvB"
                     /locus_tag="ACHOTW_001511"
     CDS             742..1752
                     /gene="ruvB"
                     /locus_tag="ACHOTW_001511"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9843642.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1998..2783)
                     /gene="znuB"
                     /locus_tag="ACHOTW_001512"
     CDS             complement(1998..2783)
                     /gene="znuB"
                     /locus_tag="ACHOTW_001512"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP9843643.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2780..3535)
                     /gene="znuC"
                     /locus_tag="ACHOTW_001513"
     CDS             complement(2780..3535)
                     /gene="znuC"
                     /locus_tag="ACHOTW_001513"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP9843644.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3614..4546
                     /gene="znuA"
                     /locus_tag="ACHOTW_001514"
     CDS             3614..4546
                     /gene="znuA"
                     /locus_tag="ACHOTW_001514"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP9843645.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4562..5884
                     /gene="mepM"
                     /locus_tag="ACHOTW_001515"
     CDS             4562..5884
                     /gene="mepM"
                     /locus_tag="ACHOTW_001515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP9843646.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6004..6975
                     /gene="lpxM"
                     /locus_tag="ACHOTW_001516"
                     /gene_synonym="msbB"
     CDS             6004..6975
                     /gene="lpxM"
                     /locus_tag="ACHOTW_001516"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9843647.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7106..8548)
                     /gene="pyk"
                     /locus_tag="ACHOTW_001517"
     CDS             complement(7106..8548)
                     /gene="pyk"
                     /locus_tag="ACHOTW_001517"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9843648.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8676..9545)
                     /gene="hexR"
                     /locus_tag="ACHOTW_001518"
                     /gene_synonym="yebK"
     CDS             complement(8676..9545)
                     /gene="hexR"
                     /locus_tag="ACHOTW_001518"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP9843649.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9883..11358
                     /gene="zwf"
                     /locus_tag="ACHOTW_001519"
     CDS             9883..11358
                     /gene="zwf"
                     /locus_tag="ACHOTW_001519"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9843650.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11593..13404
                     /gene="edd"
                     /locus_tag="ACHOTW_001520"
     CDS             11593..13404
                     /gene="edd"
                     /locus_tag="ACHOTW_001520"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP9843651.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            13441..14082
                     /gene="kdgA"
                     /locus_tag="ACHOTW_001521"
                     /gene_synonym="eda"
     CDS             13441..14082
                     /gene="kdgA"
                     /locus_tag="ACHOTW_001521"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9843652.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14138..15316)
                     /gene="purT"
                     /locus_tag="ACHOTW_001522"
     CDS             complement(14138..15316)
                     /gene="purT"
                     /locus_tag="ACHOTW_001522"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP9843653.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            15450..15740
                     /gene="yebG"
                     /locus_tag="ACHOTW_001523"
     CDS             15450..15740
                     /gene="yebG"
                     /locus_tag="ACHOTW_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP9843654.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            15807..16163
                     /gene="yebF"
                     /locus_tag="ACHOTW_001524"
     CDS             15807..16163
                     /gene="yebF"
                     /locus_tag="ACHOTW_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP9843655.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16490..17149
                     /gene="yebE"
                     /locus_tag="ACHOTW_001525"
     CDS             16490..17149
                     /gene="yebE"
                     /locus_tag="ACHOTW_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HFP9843656.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17358..19418
                     /gene="ptrB"
                     /locus_tag="ACHOTW_001526"
     CDS             17358..19418
                     /gene="ptrB"
                     /locus_tag="ACHOTW_001526"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP9843657.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(19415..20077)
                     /gene="exoX"
                     /locus_tag="ACHOTW_001527"
     CDS             complement(19415..20077)
                     /gene="exoX"
                     /locus_tag="ACHOTW_001527"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP9843658.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20101..20757)
                     /gene="yobB"
                     /locus_tag="ACHOTW_001528"
     CDS             complement(20101..20757)
                     /gene="yobB"
                     /locus_tag="ACHOTW_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP9843659.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(20859..21089)
                     /gene="holE"
                     /locus_tag="ACHOTW_001529"
     CDS             complement(20859..21089)
                     /gene="holE"
                     /locus_tag="ACHOTW_001529"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP9843660.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21228..21602
                     /gene="yobA"
                     /locus_tag="ACHOTW_001530"
     CDS             21228..21602
                     /gene="yobA"
                     /locus_tag="ACHOTW_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP9843661.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21606..22478
                     /gene="copD"
                     /locus_tag="ACHOTW_001531"
     CDS             21606..22478
                     /gene="copD"
                     /locus_tag="ACHOTW_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP9843662.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            22491..22832
                     /gene="yebY"
                     /locus_tag="ACHOTW_001532"
     CDS             22491..22832
                     /gene="yebY"
                     /locus_tag="ACHOTW_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP9843663.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            23228..23884
                     /gene="pphA"
                     /locus_tag="ACHOTW_001533"
     CDS             23228..23884
                     /gene="pphA"
                     /locus_tag="ACHOTW_001533"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9843664.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(23885..24076)
                     /gene="yebW"
                     /locus_tag="ACHOTW_001534"
     CDS             complement(23885..24076)
                     /gene="yebW"
                     /locus_tag="ACHOTW_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP9843665.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(24181..24417)
                     /gene="yebV"
                     /locus_tag="ACHOTW_001535"
     CDS             complement(24181..24417)
                     /gene="yebV"
                     /locus_tag="ACHOTW_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP9843666.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(24535..25974)
                     /gene="rsmF"
                     /locus_tag="ACHOTW_001536"
     CDS             complement(24535..25974)
                     /gene="rsmF"
                     /locus_tag="ACHOTW_001536"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP9843667.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(26055..28688)
                     /gene="yebT"
                     /locus_tag="ACHOTW_001537"
     CDS             complement(26055..28688)
                     /gene="yebT"
                     /locus_tag="ACHOTW_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP9843668.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(28657..29940)
                     /gene="yebS"
                     /locus_tag="ACHOTW_001538"
     CDS             complement(28657..29940)
                     /gene="yebS"
                     /locus_tag="ACHOTW_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP9843669.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            30070..30567
                     /gene="msrC"
                     /locus_tag="ACHOTW_001539"
     CDS             30070..30567
                     /gene="msrC"
                     /locus_tag="ACHOTW_001539"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP9843670.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            30664..31362
                     /gene="proQ"
                     /locus_tag="ACHOTW_001540"
     CDS             30664..31362
                     /gene="proQ"
                     /locus_tag="ACHOTW_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP9843671.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            31382..33430
                     /gene="prc"
                     /locus_tag="ACHOTW_001541"
     CDS             31382..33430
                     /gene="prc"
                     /locus_tag="ACHOTW_001541"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP9843672.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            33622..34503
                     /gene="htpX"
                     /locus_tag="ACHOTW_001542"
     CDS             33622..34503
                     /gene="htpX"
                     /locus_tag="ACHOTW_001542"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP9843673.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(34549..35922)
                     /gene="yebQ"
                     /locus_tag="ACHOTW_001543"
     CDS             complement(34549..35922)
                     /gene="yebQ"
                     /locus_tag="ACHOTW_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9843674.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            36099..36890
                     /gene="kdgR"
                     /locus_tag="ACHOTW_001544"
     CDS             36099..36890
                     /gene="kdgR"
                     /locus_tag="ACHOTW_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP9843675.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(37034..37273)
                     /gene="yobH"
                     /locus_tag="ACHOTW_001545"
     CDS             complement(37034..37273)
                     /gene="yobH"
                     /locus_tag="ACHOTW_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP9843676.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            37433..37576
                     /gene="mgrB"
                     /locus_tag="ACHOTW_001546"
     CDS             37433..37576
                     /gene="mgrB"
                     /locus_tag="ACHOTW_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP9843677.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            37651..37938
                     /gene="yebO"
                     /locus_tag="ACHOTW_001547"
     CDS             37651..37938
                     /gene="yebO"
                     /locus_tag="ACHOTW_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP9843678.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT         9762 a         9384 c         9825 g         9145 t
ORIGIN      
        1 actgtgccat ttttcagttc atcgagacac ctcgcaagtt tttttcatcc ttcgctggat
       61 atctatccag cattttttta tcatacagca ttatctttga ttcattacgc aggagcgtca
      121 tgtgataggc agactcagag gcatcatcat tgaaaaacaa cccccgctgg tgttaattga
      181 agtgggcggc gtaggctatg aagtgcatat gccgatgacc tgtttttatg aactccctga
      241 agcgggtcag gaagcgatcg ttttcaccca ctttgtggtg cgtgaagacg cgcaactgct
      301 gtacggtttt aacaataaac aagagcgcac gttgttcaaa gagttgatca aaaccaacgg
      361 cgtcggcccg aagttggcgc tggcgatcct ctccggaatg tcagcgcagc agttcgttaa
      421 tgccgttgag cgtgaagaag tgggggcact ggtgaaactg ccgggtattg gcaaaaaaac
      481 cgccgaacgc ttgattgttg aaatgaaaga ccgatttaaa ggtttgcatg gcgatctctt
      541 tacgccagcc gccgacctgg tactcacgtc tcctgccagc ccggcgaccg acgatgctga
      601 acaagaagcg gttgccgcgc tggtggcgct gggctataaa ccacaagaag caagccgcat
      661 ggtgagcaaa atcgctcgcc ctgacgccag cagtgaaaca ttaattcgcg aagccctacg
      721 cgccgcgtta tgaggtaaag gatgattgaa gcagaccgtc tgatttctgc cggtaccact
      781 ttgccggaag atgtagcaga tcgcgccatt cgccctaaat tactggaaga gtatgttggt
      841 cagccgcagg ttcgttcaca gatggagatt ttcatcaaag cagcgaaact gcgcggcgat
      901 gccctcgatc atttgttgat ttttggtcct ccggggttgg gtaaaactac gcttgccaac
      961 attgtcgcca atgaaatggg cgttaattta cgcacgactt ctggtccggt gctggaaaag
     1021 gcgggcgatt tggctgcgat gctcactaac cttgaaccgc atgacgtgct gtttattgat
     1081 gagatccacc gtttatcgcc agttgttgaa gaagtgctgt acccggcaat ggaagactac
     1141 caactggata tcatgattgg tgaaggtccg gcggcacgct ccattaaaat tgatttgccg
     1201 ccgtttaccc tgattggtgc aaccacgcgc gcaggttcgc tgacatcacc gttgcgcgac
     1261 cgttttggta ttgtgcaacg tctggagttt tatcaggtgc cggatctgca atatatcgtc
     1321 agtcgcagcg cacgctttat ggggcttgag atgagtgatg acggcgcgct ggaagttgct
     1381 cgtcgcgctc gcggtacgcc gcgcattgcc aaccgtctgc tgcgtcgagt gcgtgatttc
     1441 gccgaagtga agcacgatgg caccatctcg gcagatatcg ctgctcaggc gctggatatg
     1501 ttgaatgtcg atgctgaagg tttcgattat atggaccgca aattgttgct ggcggtaatc
     1561 gataagttct ttggtgggcc ggtaggtctg gataaccttg cggcagccat tggcgaagaa
     1621 cgtgaaacca ttgaggatgt gctggaacct tatttgattc agcaaggctt tttgcagcgt
     1681 acaccgcgtg ggcgtatggc gacgacgcgg gcgtggaatc actttggcat aacgccgcca
     1741 gaaatgccgt aagtcggatt ggtcgctcgc accgcatccg acattggcag tgtgcctgat
     1801 gcgacgctat tcgcgtctta tcaggcatat accagagata gcgaaccgta ggtcggaaaa
     1861 ggcgctcgcg ccgcatccga cattggcagt gtgcctgatg cgacgctgtt cgcgtcttac
     1921 caggcctacc agagatagcg aaccgtaggt cggataaggc gctcgcgccg catccgacaa
     1981 atgtgttcag cgatagatta gctggcctgc tttttcatca tactgataat aaataacagt
     2041 gccgcacata gcaccaccga cggacccgcc ggcgtatcgt aaaacgcgga aaaggttaaa
     2101 ccgtcagtta ctgccaccat ccccaccaaa acagcgacac cagccatctg ttccggcgta
     2161 cgggcaaagc gacgcgcagt agctgcagga ataatcagca acgaagtaat aatcaacgca
     2221 ccgacaaatt tcatcgctac accaatcgtc aatgccgtca ccagcatcaa caacaatttc
     2281 actcgctgta atttcacacc atcaacaaac gccagatccg ggctaatcgt catcgacagc
     2341 aaattgcgcc attgccagaa caaaatagcc accacgatga ccacgccaat tgcaatagag
     2401 atgagatctt ctggcgtcac tgccagtaaa tcaccgaaca ggtaagccat caaatcaaca
     2461 cgaatgttag acatcagact aacgaccacc aggcccagcg acagggcact gtgcgccata
     2521 atccctaata acgtgtcgat cgccagctgt ggacgcttct ccagccatac cagaccgccc
     2581 gccagcagca gcgtaacggc aatcaccgca tagaatggat tcacgtccag caacaaacca
     2641 aacgcgacac caagtaatga ggcatgagcc agcgtatcac cgaaataaga catacgacgc
     2701 cagactacaa acgaacccag cggacccgcg gcacaggcga gcatgatccc ggctaaccaa
     2761 ccgggaaata ataattcaat catgagcgat catttccccg acgcaaaaca attcgtccct
     2821 gtaaatcgtg acgatgatta tgatgatggc gatagatacc cagttgttca gcaccacgag
     2881 gaccaaacat tgaaataaac tccggatgca gggaaacaac ttccggtgtg ccggaacaac
     2941 aaatgtgatg attcaggcac agcacttcat cggtttttgc catcaccaga tgaagatcgt
     3001 gagaaaccat taaaacgcca caatccagtt cacgacgcag ttggtcaata aggtcatata
     3061 acgccacctg gccatttaca tccacgcctt gagtgggttc atccagcact aataattgcg
     3121 ggcgatttaa caatgctcgc gctaacagta cacgctgcgt ttcgccacca gagagctttt
     3181 gcatcggtgc gttaatcagg tgcccggcct ggacacgttt cagtgcaggc aaaatatctt
     3241 ctttatgtgt accagggcgt aagcgtaaaa aacggtttac ggtcagtggc aacgtggtgt
     3301 cgagatacag cttctgcggt acatagccga tgcgcagttt tccgttgcgc ttgataaccc
     3361 cttcatcggg tgttaccagc ccgagcacta cccgtaccag tgtcgactta cctgcgccat
     3421 ttggcccaag taaagtcaaa atttttccag gtttaagttc cagcgacaca tcagagagga
     3481 cgcggcgttg gccaaaagaa accgagacat tttccaggga aaccagactt gtcatgttaa
     3541 ttttagtctt gcagtagtca tgaaatgtta taatatcaca cttctcatgt tcattacgat
     3601 gattggtcgc attatgttac ataaaaaaac gcttcttttc gcagcattat tcgccgctct
     3661 ctggggcggt gcaacacagg ccgcagatgc cgccgttgtc gcttcgctta aacccgttgg
     3721 gttcatcgct tctgccattg ctgatggggt aacagaaacg gaggttttac ttcctgacgg
     3781 cgcttcagaa catgattatt cactgcgtcc atcggatgta aaacgcttac agaacgcgga
     3841 cttagtcgtt tgggttggcc cggagatgga agcgtttatg caaaaaccgg taagcaaatt
     3901 accagaagcg aagcaggtaa cgattgcgca gcttgaagat gtgaaaccgc tactgatgaa
     3961 aagtattaac ggcgatgatg atgatcacga ccacgcggaa aaaagtgacg aagatcacca
     4021 tcacggcgat ttcaacatgc atctttggct ttccccagag atagcgcggg ctacagcggt
     4081 tgcaatccat ggaaaattag tggaacttat gccgcaaagt cgagccaaac ttgacgccaa
     4141 cctgaaggat tttgaggcac aattagcctc aaccgaaacg caggttggta acgagctcgc
     4201 gccgctcaag gggaaaggtt atttcgtttt tcacgatgct tacggctatt tcgaaaaaca
     4261 gttcggactg acaccgcttg gtcattttac cgttaaccct gagattcaac ctggcgcgca
     4321 gcgtttacat gaaataagaa cacagttggt tgagcaaaaa gcaacctgcg tttttgctga
     4381 gccacagttc aggccagcgg tcgttgagag cgtcgcacga gggacatccg ttcgtatggg
     4441 aacgttggat cccttgggga cgaatatcaa actgggtaaa acaagctatt cagaattcct
     4501 gagtcaatta gccaaccagt atgcgagctg cctgaaagga gattaatgag gaagtgatta
     4561 cgtgcaacag atagcccgct ctgtcgccct ggcgtttaat aatttaccgc gaccacaccg
     4621 cgttatgttg gggtcgctca ccgttcttac tctggccgtc gctgtctggc ggccctatgt
     4681 ttatcaccgc gatgccacgc caattgtcaa aaccattgag ctggaacaga acgaaattcg
     4741 ttcgctctta cctgaagcca gtgagccgat tgatcaagct gcacaagaag atgaagccat
     4801 tccccaggac gaactggatg acaaaatcgc cggtgaagcg ggcgtgcatg aatatgttgt
     4861 ttccactggc gatacgctaa gcagcattct caatcagtat ggtattgata tgggtgatat
     4921 cacccaactg gctgcggccg acaaagaatt gcgtaacctg aaaatcggtc aacaactctc
     4981 ctggacatta accgcggacg gcgaactgca gcgcctcacc tgggaagtgt ctcgtcgtga
     5041 aacccgaacc tatgaccgta ctgccgctaa cggttttaaa atgaccagcg aaatgcagca
     5101 aggagagtgg gttaacaatc tgctgaaagg taccgtcggg gggagctttg ttgccagcgc
     5161 cagaaacgcc ggtttgacca gcgccgaagt gagcgcagtg attaaagcca tgcagtggca
     5221 aatggatttc cgcaaactga aaaaaggcga tgaatttgcg gtattaatgt ctcgagaaat
     5281 gcttgatggt aaacgtgagc aaagccagct gctgggcgta cgtttgcgtt cagaaggtaa
     5341 agattattac gctatccgcg ctgaagatgg caaattctac gatcgtaacg gtactggtct
     5401 ggcgaaagga ttcttgcgat tcccgacggc gaaacagttc cgtatctcgt ctaactttaa
     5461 cccgcgtcgt actaatccgg tgaccggtcg cgttgcacca cacagaggtg ttgatttcgc
     5521 catgccgcag ggtacgccag tgctttcagt gggtgacggt gaagtggtgg ttgccaaacg
     5581 cagtggcgca gcaggttatt atgtggctat tcgtcatggt cgcagctaca ccacgcgtta
     5641 tatgcacttg cgcaagattc tggtgaaacc ggggcagaag gtgaaacgtg gcgaccgtat
     5701 cgcgctttcc ggtaataccg gacgttcaac cgggccgcat ctgcactatg aagtatggat
     5761 aaaccagcag gccgtaaacc cgctgacggc aaaactgccg cgtaccgaag ggctgaccgg
     5821 ctccgatcgt cgcgaattcc tggcgcaggc caaagagatt gtgccgcagc tacggtttga
     5881 ttaattaaca tccattcgca gccggtacgc agtcagtacc ggcttttttt atttggtgcg
     5941 gggcaagttg cgccgctaca ctatcaccag attgattttt gccttatccg aaactggaaa
     6001 agcatggaaa cgaaaaaaaa taatagcgaa tacattcctg agtttgataa atcctttcgc
     6061 cacccgcgct actggggagc atggctgggc gtagcagcga tggcgggtat cgctttaacg
     6121 ccgccaaagt tccgtgatcc cattctggca cggctgggac gtattgccgg acgactggga
     6181 aaaagctcac gccgtcgtgc gttaatcaat ctgtcgctct gctttccaga acgtagtgaa
     6241 gctgaacgcg aagcgattgt tgatgagatg tttgccaccg cgccgcaagc gatggcaatg
     6301 atggctgagt tggcaatacg cgggccggag aaaattcagc cgcgcgttga ctggcaaggg
     6361 ctggagatca tcgaagagat gcggcgtaat aacgagaaag ttatctttct agtgccgcac
     6421 ggttgggccg tcgatattcc tgccatgctg atggcctcgc aagggcagaa aatggcagcg
     6481 atgttccata atcagggcaa cccggttttt gattatgtct ggaacacggt gcgtcgtcgc
     6541 tttggcggtc gtctgcatgc gagaaatgac ggtattaaac cattcatcca gtcggtacgt
     6601 caggggtact ggggatatta tttacccgat caggatcatg gcccagagca cagcgaattt
     6661 gtggatttct ttgccaccta taaagcgacg ttgcccgcga ttggtcgttt gatgaaagtg
     6721 tgccgtgcgc gcgttgtacc gctgtttccg atttatgatg gcaagacgca tcgtctgacg
     6781 attcaggtgc gcccaccgat ggatgatctg ttagaggcgg atgatcatac gattgcgcgg
     6841 cggatgaatg aagaagtcga gatttttgtt ggtccgcgac cagaacaata cacctggata
     6901 ctaaaattgc tgaaaactcg caaaccgggc gaaatccagc cgtataagcg caaagatctt
     6961 tatcccatca aataaaaaag cctctcgcga ggagaggcct tcgcctgatg ataagttcaa
     7021 gtttgcttca gaatattcga aatctgttga actatcattg aactgtaggc cggatgtggc
     7081 gttttcgccg catccggcaa cgtacttact ctaccgttaa aatacgcgtg gtattagtag
     7141 aacccacggt actcatcacg tcgccctggg tgacaatcac caggtcacca gacatcaagt
     7201 aacctttatc gcgcagcaga ttaaccgctt cgctggcagc tgctacgccg tcattagcgc
     7261 tatcaaagtg caccggcgta acgccacgat agagagcagt caggttcagc gtacgttcat
     7321 ggcgcgacat ggcgaaaatt ggtagaccag agctgatacg ggaggtcatc agcgcggtac
     7381 gacccgattc ggtcatggta atgatcgccg taacgccttt caggtggtta gctgcgtaca
     7441 ttgctgacat ggcaatagct tcttccacat tgtcgaactg aacgtccaga cggtgtttag
     7501 aaacgttgat gctcgggatt ttttccgcac ccaggcaaac gcgcgccatg gctgcaacag
     7561 tttctgacgg atactgccca gcggcagttt ctgcagacag catcacagcg tcagtaccat
     7621 ccagaacggc gtttgctacg tccatgactt ctgcacgcgt cggcatcggg ttagtaatca
     7681 ttgactccat catctgggtc gccgtgatta ccgctcggtt tagctgacgc gcacgacgga
     7741 tcaacgcttt ctgaatgccg accagttccg ggtcgccaat ttccacaccg aggtcgccac
     7801 gtgcaaccat taccacgtca gaggcgagga tgatgtcatc cattgcatcc tggctgcaaa
     7861 cggcttccgc acgttcaacc ttggcaacaa ttttcgcatc acatcctgca tcgcgtgcca
     7921 ggcgacgggc atagttcaga tcttcgccac agcgtgggaa ggagacagcc aggtaatcta
     7981 cgccaatcaa cgccgcagtc ttaatgtctg ctttgtcttt ttcggtcagc gcttcagccg
     8041 acaaaccgcc gccaagtttg ttgatacctt tattgttgga gaggggacca ccgacggtga
     8101 cttcggtgaa cactttcatg ccctgaactt ccagtacttt taactggacg cgaccatcgt
     8161 ccagcagcag gatgtcacca ggcacgacgt cagcaggcag gcctttgtag ttgataccga
     8221 ctttttcttt gtcgccttca cctttaccca ggttggcgtc gagcaggaat ttatccccaa
     8281 tattgaggaa aactttgcct tctttaaagg tggatacacg gattttgggc ccctggaggt
     8341 cacccagaat agccacatga cgccccagtt ttgcggcaat ctcacgaact ttatccgcgc
     8401 gcattttgtg atcttcaggc gagccgtgag aaaagttcat acgtacaacg ttggcacccg
     8461 ccgcgataac tttttcaaga ttattatcgc gatctgttgc tgggcctaac gtggtaacga
     8521 tttttgttct gcgaagcctt ctggacatgt aatactccgt tgactgaaac aaccaggtgt
     8581 tgcttgaaca tgaaatccga atgaaataac gccgcgatgg cgataaaata atctcattcg
     8641 gatgtcgtat aagattagaa cagtgacagt cgcttttagc gatcgtcact taaattaagt
     8701 aactgcttat caaaacgcga ttccttcagc gcttccttga cccgcttcaa gttatctctg
     8761 aattttgcac cgcgtcgcaa agtaaatcct gtcgccagca catctatcac ggtcagctgt
     8821 gcaagtcgag aaaccatggg catataaatg tcagtatctt ccggtacgtc gagggtaatt
     8881 gccagcgttg cttcccgggc gagcggggta cccgcagagg tgagggcaat caccatagcg
     8941 tcgttttcgc gtgccagctg cgccagctcg accagatttt ttgttcttcc ggtgtgagaa
     9001 atcagcacca ccacgtctcc gtcgctacaa ttcatacaac tcatgcgttg cagcacaata
     9061 tcatcggagt acaccaccgg aacattaaaa cgaaagaact tattcatcgc atcgtgggca
     9121 acggcggctg aagagcctaa tccgaaaaag gcgatttttt ttgcctgagt gagcaagtcg
     9181 acggcgcggt tgatggcaga tttatccagt gaatgacgga catgatcgag cgttgccatt
     9241 gcggactcaa atattttccc tgtgtatgat tcaacgctgt catcttcatt gacgttgcga
     9301 ttaacatagg gagtgccatt cgccagactc tgtgccagat gaagtttaaa atcaggaaaa
     9361 ccgcgcgtgt ccatgctgcg acagaaacga ttcaccgtcg gttcgctaac attggcttcc
     9421 agtgccagag cagcaatact cgaatggatc gcgttatcgg gcgaagccag aatgacctcg
     9481 gcaactttgc gctctgattt gctcaaatgt tccagctgag actggatttt ttccagcata
     9541 ttcatgatgt aaagagactc acgggtaatg acgatttccg cactgaaaga aatcgaaatg
     9601 cagttttgtc agatattacg cctgtgtgct gtgttaatga caaaagcaga taaaaaagtt
     9661 gttatttttt ttcataacat gatcagtgtc agatttttac ccaatggaaa acgatgattt
     9721 ttttatcagt tttgccgcac tttgcgcgct tttaccgtaa tcgcacgggt ggataagcgt
     9781 ttacagtttt cgcaagctcg taaaagcagt acagtgcacc gtaagaaaat tacaagtata
     9841 ccctggctta agtaccgggt tagttaactt aaggagaatg acatggcggt aacgcaaaca
     9901 gcccaggtct gtgacttggt cattttcggc gcgaaaggcg accttgcgcg tcgtaaattg
     9961 ctgccttccc tgtatcaact ggaaaaagcc ggtcagctca acccggacac ccggattatc
    10021 ggcgtagggc gtgctgactg ggataaagcg gcatatacca aagttgtccg cgaggcgctc
    10081 gaaactttca tgaaagaaac cattgatgaa ggtttatggg acaccctgag cgcacgtctg
    10141 gatttttgta atctcgatgt caatgacact gctgcattca gccgtctcgg cgcgatgctg
    10201 gatcaaaaaa atcgtatcac cattaactac tttgccatgc cgcccagcac ttttggcgca
    10261 atttgcaaag ggcttggcga ggcaaaactg aatgctaaac cggcacgcgt agtcatggag
    10321 aaaccgctgg ggacgtcgct ggcgacctcg caggaaatca atgatcaggt tggcgaatac
    10381 ttcgaggagt gccaggttta ccgtatcgac cactatcttg gtaaagaaac ggtgctgaac
    10441 ctgttggcgc tgcgttttgc taactccctg tttgtgaata actgggacaa tcgcaccatt
    10501 gatcatgttg agattaccgt ggcagaagaa gtggggatcg aagggcgctg gggctatttt
    10561 gataaagccg gtcagatgcg cgacatgatc cagaaccacc tgctgcaaat tctttgcatg
    10621 attgcgatgt ctccgccgtc tgacctgagc gcagacagca tccgcgatga aaaagtgaaa
    10681 gtactgaagt ctctgcgccg catcgaccgc tccaacgtac gcgaaaaaac cgtacgcggg
    10741 caatatactg cgggcttcgc ccagggcaaa aaagtgccgg gatatctgga agaagagggc
    10801 gcgaacaaga gcagcaatac agaaaccttc gtggcgatcc gcgtcgacat tgataactgg
    10861 cgctgggccg gtgtgccatt ctacctgcgt actggtaaac gtctgccgac caaatgttct
    10921 gaagtcgtgg tctatttcaa aacacctgaa ctgaatctgt ttaaagagtc gtggcaggat
    10981 ctgccgcaga ataaactgac tatccgtctg caacctgatg aaggcgtgga tatccaggta
    11041 ctgaataaag ttcctggcct tgaccacaaa cataacctgc aaatcaccaa gctggatctg
    11101 agctattcag aaacctttaa tcagacgcat ctggcggatg cctatgaacg tttgctgctg
    11161 gaaaccatgc gtggtattca ggcactgttt gtacgtcgcg atgaagtgga agaagcctgg
    11221 aaatgggtag actccattac tgaggcgtgg gcgatggaca atgatgcgcc gaaaccgtat
    11281 caggccggaa cctggggacc cgttgcctcg gtggcgatga ttacccgtga tggtcgttcc
    11341 tggaatgagt ttgagtaata tctgcgctta tcctttatgg ttattttacc ggtaacatga
    11401 tcttgcacag attgtagaac aatttttaca ctttcaggcc tcgtgcggat tcacccacga
    11461 ggcttttttt attacactga ctgaaacgtt tttgccctat gagctccggt tacaggcgtt
    11521 tcactcataa atcctctgaa tgaaacgcgt tgtgaatcat cctgctctga caactcaatt
    11581 tcaggagcct ttatgaatcc acaattgtta cgcgtaacaa atcgaatcat tgaacgttcg
    11641 cgcgagactc gctctgctta tctcgcccgg atagaacaag cgaaaacgtc gaccgttcat
    11701 cgttcgcagt tggcatgcgg taacctggca cacggtttcg ctgcctgcca gccagaagac
    11761 aaagcctctt tgaaaagcat gttgcgtaac aatatcgcca tcatcacctc ctataacgac
    11821 atgctctccg ctcaccagcc ttatgaacac tatccagaaa tcattcgtaa agccctgcat
    11881 gaagcgaatg cggttggtca ggttgcgggc ggtgttccgg cgatgtgtga tggtgtcacc
    11941 caggggcagg atggaatgga attgtcgctg ctaagccgcg aagtgatagc gatgtctgcg
    12001 gctgtcgggc tgtcccataa catgtttgat ggtgctctgt tcctcggggt gtgcgacaag
    12061 attgtcccgg gtctgacgat ggcagccctg tcgtttggtc atttgcctgc ggtgtttgtg
    12121 ccgtctggac cgatggcaag cggtttgcca aataaagaaa aagtgcgtat tcgccagctt
    12181 tatgccgaag gtaaagtgga ccgcatggcc ttactggagt cagaagccgc gtcttaccat
    12241 gcgccgggaa catgtacttt ctacggtact gccaacacca accagatggt ggtggagttt
    12301 atggggatgc agttgccagg ctcttctttt gttcatccgg attctccgct gcgcgatgct
    12361 ttgaccgctg ccgctgcgcg tcaggttaca cgcatgaccg gtaatggtaa tgaatggatg
    12421 ccgatcggta agatgatcga tgagaaagtg gtggtgaacg gtatcgttgc actgctggcg
    12481 accggtggtt ccactaacca caccatgcac ctggtggcga tggcgcgcgc ggccggtatt
    12541 cagattaact gggatgactt ctctgacctt tctgatgttg taccgctgat ggcacgtctc
    12601 tacccgaacg gtccggccga tattaaccac ttccaggcgg caggtggcgt accggttctg
    12661 gtgcgtgaac tgctcaaagc aggcctgctg catgaagatg tcaatacggt ggcaggcttt
    12721 ggtctgtctc gttataccct tgaaccatgg ctgaataatg gtgaactgga ctggcgggaa
    12781 ggggcggaaa aatcactcga cagcaatgtg atcgcttcct tcgaacaacc tttctctcat
    12841 catggtggga caaaagtgtt aagcggtaac ctgggccgtg cggttatgaa aacctctgcc
    12901 gtgccggttg agaaccaggt gattgaagcg ccagcggttg tttttgaaag ccagcatgac
    12961 gttatgccgg cctttgaagc gggtttgctg gaccgcgatt gtgtcgttgt tgtccgtcat
    13021 caggggccaa aagcgaacgg aatgccagaa ttacataaac tcatgccgcc acttggtgta
    13081 ttattggacc ggtgtttcaa aattgcgtta gttaccgatg gacgactctc cggcgcttca
    13141 ggtaaagtgc cgtcagctat ccacgtaaca ccagaagctt acgatggcgg gctgctggca
    13201 aaagtgcgcg acggggacat cattcgtgtg aatggacaga caggcgaact gacgctgctg
    13261 gtagacgaag cggaattggc tgctcgcgaa ccacacattc ctgacctgag cgcgtcacgc
    13321 gtgggaacag gacgtgaatt attcagcgcc ttgcgtgaaa aactgtccgg tgccgaacag
    13381 ggcgcaacct gtatcacttt ttaagacgac aaatttgtaa tcaggcgaga gaaaactctg
    13441 atgaaaaact ggaaaacaag tgcagaatca atcctgacca ccggcccggt tgtaccggtt
    13501 atcgtggtaa aaaaactgga acacgcggtg ccgatggcaa aagcgttggt tgctggtggg
    13561 gtgcgcgttc tggaagtgac tctgcgtacc gagtgtgcag ttgacgctat ccgtgctatc
    13621 gccaaagaag tgcctgaagc gattgtgggt gccggtacgg tgctgaatcc acagcagctg
    13681 gcagaagtca ctgaagcggg tgcacagttc gcaattagcc cgggtctgac cgagccgctg
    13741 ctgaaagctg ctaccgaagg gactattccg ctgattccgg ggatcagcac tgtttccgaa
    13801 ctgatgctgg gtatggacta cggtttgaaa gagttcaaat tcttcccggc tgaagctaac
    13861 ggcggcgtga aagccctgca ggcgatcgcg ggtccgttct cccaggtccg tttctgcccg
    13921 acgggtggta tttctccggc taactaccgt gactacctgg cgctgaaaag cgtgctgtgc
    13981 atcggtggtt cctggctggt tccggcagat gcgctggaag cgggcgatta cgaccgcatt
    14041 actaagctgg cgcgtgaagc tgtagaaggc gctaagctgt aaaagtcaaa atgcccgatc
    14101 gaggatcggg catttttgta gcgtttattt gccgggttta accctgtact tttacctgtc
    14161 cggcggcgtg cttcgcgcgt tcaatggcgt caacaacact ctctgcagta gccagtgcca
    14221 cccccagacg acggctgcca tcaatttccg gcttaccaaa taaacgaatc tgcaaatctg
    14281 cgcctacggc attctgcaca ttatcaaacg tgacattctg actggtcagt tgtggcagaa
    14341 taacggcaga agctgcagga ccatactgac ggatcccgcc aaccggaagt ccgaggaagg
    14401 cacgtacatg cagggcaaac tctgagagat cttgagaaat taacgtcacc atcccggtat
    14461 catgtggacg aggggagacc tcactgaaaa tcacctcatc accacagaca aacagctcga
    14521 caccaaacag accataaccg cccagtgcca gcaccacttt acgggcaatc tcctgcgcac
    14581 gttcaagggc aagcgggctc atttgctgtg gttgccagga ttcacagtag tcgccatctt
    14641 cctggcgatg acctactggt gcacagaaat ggacgccatc caccgcgctg acggttagca
    14701 gggtaatttc gaagtcaaac ttaacgacgc cttcaacaat tacgcggccc gctccggcgc
    14761 gaccgccttg ctgagcgtat tcccatgcct gagcaagttg ctctgcagag cgaataaacg
    14821 tctgcccctt gccggaagag ctcatcaccg gttttacaat gcagggatag ccaatggcgg
    14881 caaccgcctc gcggaaaagg ctttcgctat cggcaaaacg ataagtggaa gtgggcagct
    14941 gcagctcttc tgccgccagg cgacggatac cctcgcgatt catcgttaat ttcgttgcgc
    15001 gagcgcaggg gacaacattc agtccttcct cttcaagttg gatcagcata tcggtggcaa
    15061 tagcttcgat ctccggcacg atataatgtg gtttttccag ttcaaccaca cggcgtaatg
    15121 catcaccatc aagcatatta atgacatggg agcgatgcgc gacatgcatg gctggtgcgt
    15181 cggcatagcg atcgacggca atcacctcta cgccgagacg ctgacactcg attgccactt
    15241 ctttacccag ttcaccggag cctaataaca tcacgcgagt tgctgccgga cgcagcgcag
    15301 tgcctaataa cgtcataacg aatatcccct gattaattcc gcgcgcagta taaacgaaaa
    15361 cgtttgcgtg tgtctttatt gctggtgttg atctctcaac cgaaaagaaa tatactgtat
    15421 agaatcacag ttattatgag aggctgcaaa tggcggttga agttaaatac gtagtcattc
    15481 gtgagggcga agagaaaatg tcgtttacca gcaaaaagga agccgatgct tatgacaaaa
    15541 tgcttgatac ggcggatctg cttgacacct ggctgacaaa ttctccagtg caaatggaag
    15601 acgagcaacg tgaagccctt tcgctatggc tggcagaaca aaaagatgtg ctgagcacca
    15661 ttctgaaaac cggcaaatta ccgtccccac aggtcgtcgg tgctgaatcc gaagaggaag
    15721 atgcctcaca cgcggcctga agcaacttgc gctcctgaca atttgtacca tgctttctcc
    15781 tgaatcattg tgctatggag aaaaacatga aaaaaagagg ggcgttttta gggctgttgt
    15841 tggtttctgc ctgcgcatca gttttcgctg ccaataatga aaccagcaag tcggtcactt
    15901 tcccaaagtg tgaaggtctg gatgctgccg gaattgccgc gagcgtaaaa cgtgattatc
    15961 aacaaaatcg cgtggcgcgc tgggcagatg atcaaaaaat tgtcggtcag gccgatcccg
    16021 tggcatgggt cagtttgcag gacattcagg gtaaagatga taaatggtca gtaccgctaa
    16081 ccgtacgtgg taaaagtgcc gatattcatt accaggtcag cgtggactgc aaagcgggaa
    16141 tggcggaata tcagcggcgt taatgggttc tgataattcg attgaccgtt ggcggatgcg
    16201 gcgcgagtgc cttgtccgcc ccagaacgca ccacaatcgt aggcctgaga agacgcaaca
    16261 gcgtcgcatc gggcaatcag catcaaacaa tgccactcaa aacgacaaac tactttcacc
    16321 taaaataccc ttatcacctg atatacagta aataccttac ctggcgcgaa tttctttgaa
    16381 taacggccta ccgccccgct tatttgcgca tcttttgtct cgatatacat tttctgacat
    16441 cctcatcctc tatgctacag gcactaaaaa tggttattga ggagcaaata tggctaactg
    16501 gttaaatcaa ctgcaatccc tgcttgggca aagcagttct tctacctcct cgtctgcgga
    16561 tcagggattg ggcaaacttt tagtgccggg cgcattaggc ggactggctg gactgctggt
    16621 cgcaaataaa tcagcacgta aacttcttac gaaatatggc accaacgcgt tgctgattgg
    16681 cggcggagcg gtagcgggta cggtgctgtg gaataaatac aaagataaaa ttcgcgcggc
    16741 gcatcaggac gaaccgcagt ttggctcgca aagtacgccg ctggatgagc gtacggaacg
    16801 tttgatcctt gcgctagtct ttgccgctaa aagtgatggt catattgatg ccaaagaacg
    16861 tgcggcaatc gaccagcaat tgcgtgaagc cggcgtggaa gagcaggggc gggtactcat
    16921 tgagcaggca atcgaacaac cgctggatcc acaacgcctg gctaccgggg tccgcaatga
    16981 agaagaggcg ctggaaatct atttcctgag ctgcgcggct attgatattg accattttat
    17041 ggaacgcagt tacctgaatg cactgggtga cgcgctgaaa atcccccagg atgttcgtga
    17101 aggcattgaa cgcgatctcg aacagcaaaa acgcactttg gcggaataat tccgctctct
    17161 ggatagcctg aagttgacta tttcgggcta tccagaacag cacagccatt tgaacaggct
    17221 gattttctct gtctgcacaa cattcgatat tcgcgccatt acctgtcatc atctaagcaa
    17281 tgactgccct gtttcgcttg catccccggt gagttttgcc acccttataa gatgtttcaa
    17341 ccagaaagaa caataacatg ctaccaaaag ccgcccgcat tccccacgcc atgacgcttc
    17401 atggcgatac gcgcatcgat aattactact ggctgcggga cgatacgcgt tctcagccgg
    17461 aagtcctgga ctacctgcaa caagaaaata gttacggtca tcgggtgatg gcctcacaac
    17521 aagccttgca ggatcgcatc ttaaaggaaa tcatcgaccg cattccgcaa cgagaagttt
    17581 ctgcgcccta catcaaaaat ggctaccgct accggcatat ttatgaacca ggctgtgaat
    17641 atgctatcta ccagcgtcaa tcggcgttca gtgaagagtg ggacgagtgg gaaatattgc
    17701 tcgatgccaa caagcgcgcg gctcatagtg agttttattc gatgggcgga atggcgatta
    17761 cgcccgataa caccattatg gcgctggcag aagattttct ttcccgacgc cagtacggca
    17821 ttcgttttcg taatctggaa acaggtaact ggtacccgga actgctggat aacgttgaac
    17881 ccagctttgt ctgggcaaat gactcctgga ctttctacta tgttcgcaag catccggtga
    17941 cgctgctgcc ttatcaggtc tggcgtcacg ccatcggtac gccagcatcg caagataaac
    18001 tgatttacga agaaaaagac gatacctatt acgtcagcct gcataaaacg acctcaaagc
    18061 actatgtagt cattcatttg gccagcgcca ccaccagtga agttcgcctg ctggacgcgg
    18121 aaatggcaga tgccgagccg tttgtttttc tgccgcgccg caaagatcac gaatacagcc
    18181 ttgatcacta ccagcatcgt ttttatctgc gttccaaccg ccacggcaaa aactttggct
    18241 tataccgtac ccgtatgcgt gatgagcaac agtgggaaga gttaattccg ccacgcgaaa
    18301 acatcatgct ggaagggttt acgctgttta ccgactggct ggtggttgaa gagcgtcagc
    18361 gcgggttaac cagtttgcgc caaattaacc gcaagacccg ggaagtcatt ggtattgcct
    18421 ttgatgatcc agcctatgtg acctggattg cctacaatcc agaacctgaa accgcgcgat
    18481 tgcgttatgg ttattcttcc atgaccacac cagacacttt gtttgaactg gatatggata
    18541 ccggtgagcg tcgtgtatta aaacaaacag aagttcctgg ttttgatgcg gcgaattacc
    18601 gcagtgaaca cctgtggata gtcgcccgtg atggtgtcga agttccggtt tcgttggtct
    18661 accatcgcaa tcattttcgc aaaggacaca acccgctgct ggtgtatggc tacggttctt
    18721 acggcgcaag tattgatgcc gatttcagtt ttagccgctt gagtttgtta gatcgtggct
    18781 ttgtctacgc cattgtccat gttcgcggcg gcggtgagct ggggcaacaa tggtacgaag
    18841 acggtaaatt tctgaagaag aaaaatacgt ttaatgatta tcttgatgcc tgcgatgcat
    18901 tgttaaaact gggctatggc tctccttcgc tttgttatgc gatgggcggg agtgcggggg
    18961 gcatgttgat gggcgttgca attaatcaac gcccggaatt attccacggc gttatcgccc
    19021 aggtaccgtt tgttgatgtt gtaacaacga tgcttgatga atcaattcct cttaccactg
    19081 gtgagtttga agagtggggg aatccgcagg atctgcaata ttacgagtat atgaaaagct
    19141 acagcccgta tgacaacgtc accgcacagg cttatccaca tttactggta acgaccggtt
    19201 tacacgattc tcaggtgcaa tattgggaac cggcaaaatg ggtcgctaag ttgcgcgagc
    19261 tgaaaaccga taaccatctt ttattgctct gtaccgacat ggactcaggc catggcggta
    19321 aatctggtcg ctttaaatcg tacgaaggcg tagcgatgga atatgctttt ctggtcgcgc
    19381 tggcgcaggg aacattaccc gctaggcctg cggattaggt attttccaga taatgtttca
    19441 gtgttaaacg cagctccggg ctcatgctgt ccaggttatt aaacaaccag cgcagatagc
    19501 ccggatcgcg ttcggcaacg tcggaaaccg ctttgccacg gtatttgcca aaggtgaagg
    19561 tcgtcatcaa cgacggacgt ccggttatat cggccatctg ttctgccgtc cagccggagg
    19621 tgttcataat atcgataagc aacgcggcgg tgatataaca gtcatacaac gcgcggtgat
    19681 gatgcaggcc cggcggcgtc tgtacattga gcttccgtgt tttatataac gccatattgc
    19741 tgtacttgat ccctggccac aaacgacggg ccagtttcat agtgcaaatc cactcaccgg
    19801 gcatctcagg cagtacgcgg cggtcaaagc tggcgttatg cgcgacatac cattcactac
    19861 cgtaatagtg tgggatcaca tcttcaatcc acggtttatc ggcgaccatg gcttcggtga
    19921 tgcgatgaat cgccatcgct tgtggactaa taggacgatc ggggtgcacc aggtggctca
    19981 tggggttgac gattttcccg tcaatgacat caacagaggc aatctcaacg atccctccct
    20041 gcaaaccgca ggtttctgta tcgataatgc gcaacatggc atgctccagg ccgaaaaagc
    20101 ctagcgtaat ggaatgatat cgccttgcca accctgtgat gaacgctgac cgactaacaa
    20161 taatgaacca cgatcggcgc gaacgatgag acgaccatgt tcatcccata gtgcactgtt
    20221 accacgggca tttgccatca gcacagcaat cgagtactga tgtgaaaaaa attgtaagcg
    20281 acgggccgat gccagcaggt cgggttctcc cagacattgc cctgtcgtaa acagtgaaca
    20341 ggtcgggtcc atatccatac cttgcggttg ttcatcgacc accgtgatgg ttctggaacg
    20401 acggcccaga catgcaccat gactctgatg gtagatccca ggcgtttttc gccacggtgc
    20461 gaacaccgca attccacgaa taaagcgatc gttatattca acgggaaggc cggcaatgat
    20521 ggtcattcgc caggtcgttg cggcataaca aagcgggtct aacagtgaga gatcgggtgg
    20581 ggcaggaagg gcacgtcgcg aataatcaca ccccagtaaa gaaagcgatg gaaaaactaa
    20641 cagctgacat tgttgcctgg cggcggccct cacaaactcc agatgatgag ctacctgttc
    20701 ggtgagtgag gttttacgag gctcatattg cgctgctgca actttccaga acgacataat
    20761 gacttccttt tcaaaatgac tgcaaattat gggtgcggtt tagaaaatac tcataattta
    20821 gcaggcgtta tgtaagaaag tgtaactctg tataagtttt atttaagttt gggctcgtaa
    20881 ggtaaacgtg acagattgac cgaagccaaa cggtgggcaa taagccgctc gcgaaaccag
    20941 ctgcgcaaat gttcaggctg ttcacgttca accgcttcag cgatcactgg catattgtag
    21001 cgttctttaa atgccacccc ggccgccgcc aaatcgacat tcactttatc catttctgtt
    21061 tgatccagtt tagccagatt cttcagcatt cttaatctcc tcatcattcg cgggcaaaag
    21121 ttactaagag tgagcctgga tggcaagccg gaattgatga tcctcgactc agacattaga
    21181 caaggttatg ctctgtaaaa aagttattac aaaaaggaat agataatatg gcttcaactg
    21241 cacgctccct tcgctacgcg ctggcaatcc tgaccacttc gttggtgaca ccttccgtct
    21301 gggcacatgc gcatttaacg catcagtatc ctgcggcaaa cgcgcaagtg acagctgcac
    21361 cgcaggcaat cactttaaac ttctcggaag gtgttgaaac aggattcagt ggtgcaaaaa
    21421 tcacggggcc aaaaaacgaa aatattaaaa cattaccggc gaagcgaaat gagcaggacc
    21481 aaaagcgact gattgttcca ctggccgatt cgctgaaacc cggaacctac accgttgact
    21541 ggcatgttgt ttcggtggac gggcataaaa cgaaaggaca ctacaccttt agcgtgaaat
    21601 aaatcatgct ggcgtttacc tggatcgcgc tgcgatttat ccatttcacc tcgctgatgc
    21661 tggtctttgg cttcgcaatg tacggtgcct ggctggcacc cttaacgatt cgtcgcttgc
    21721 tgacgaagcg ttttctgcgt ttacaacagc acgctgccgt ctggagtttg atcagtgcca
    21781 ctgcaatgct cgccgttcag ggcgggttga tgggaacggg atggaaagat gtattttcgc
    21841 caaacatttg gcaggctgtt ttgcaaacgc agtttggtgg cgtctggtta tggcaaattg
    21901 ttctcgccct cgtaacgttg atcgtcgccc ttatgcaacc gcggaatatg ccacgtttgc
    21961 tgtttatgct cactaccgcg caatttattc tgctggcggg agtggggcat gcgacgctga
    22021 atgaaggggt aacggcgaaa atccatcaga ctaatcacgc gattcacctg atttgtgcgg
    22081 cggcgtggtt tgggggatta ctgccggtgc tctggtgtat gcaactcatc aaaggccgtt
    22141 ggcgacacca ggctattcag gcgctgatgc gtttttcctg gtgcgggcat tttgcggtga
    22201 tcggcgtact ggcaagcggc gtgcttaatg cattgttgat taccggattt ccccccacgc
    22261 ttacgactta ctggggccaa ctcttattgc tcaaagccat cctggtgatg attatggtgg
    22321 tcattgcgct ggctaatcgt tatgttcttg taccgcgcat gaggcaggat gaagatcgtg
    22381 cggcaccctg gtttgtgtgg atgactaaac tcgaatgggc gataggtgcg gtagtgctgc
    22441 tgatcatcag cctgcttgcg actctcgaac ctttttgatg gattacgaaa atgatgaaaa
    22501 aaagtattct ggcgtttctg ttactcacca gttctgcagc ggcgctggct gcaccgcagg
    22561 tgattaccgt cagccgtttt gaagtgggta aagacaaatg ggcgtttaat cgcgaagagg
    22621 tgatgctgac ttgccgaccg ggtaatgctt tgtatgtcat caacccaagt accctcgtgc
    22681 agtatccttt aaacgatgtc gcacagaagg aagttgccag tgggaagact aaagcccaac
    22741 ccatttcggt gattcagatt gatgatccca acaatcccgg cgaaaaaatg agtctggcac
    22801 cgtttataga acgagctgaa aaactctgtt aattacctaa aatagccttt tgatttccaa
    22861 taaaaaaacc gcctcagttc tttcaccaga acgggcggtt tttaacattt aagctgatga
    22921 ccaccgcgct ttttattgac cattttgcac gcaaactgga aaacctggcg tcgtcatcta
    22981 ttcttaaagg gcaaggcaac taagcctgca ttaatgccaa cttttagcgc acggctctct
    23041 cccaagagcc atttccctgg accgaataca ggaatcgtgt tcggtctctt tttatctgtt
    23101 aaaagccaga agcatttcct tcgctgactt tatagtcaac cataacacac actctactgt
    23161 ctgagtccag cgttttttaa cattcttgtt aagattatgt gatctttagc gcgggaggaa
    23221 aatattgatg aaacagcctg cgcccgttta tcagagaatt gcgggtcatc aatggcgaca
    23281 tatctggctt tctggcgata tacacggttg tcttgagcag ttgcgccgca aattatggca
    23341 ttgtcgtttt gatccgtggc gagatttact tatctcagtg ggagacgtta tcgatcgtgg
    23401 gccgcaaagt ttacgttgtc tgcagttact ggaacaacat tgggttcgtg cggtaagagg
    23461 caatcatgaa cagatggcga tggatgcgct ggcatcccag cagatgtctt tgtggttgat
    23521 gaatggcggc gactggttta ttgcgctggc agataatcaa cagaaacaag cgaaaacggc
    23581 gctggaaaaa tgtcagcatt tgccctttat tcttgaagta cacagccgca ccggcaaaca
    23641 tgttattgct catgccgatt atccagatga tgtttatgaa tggcaaaagg acgttgattt
    23701 gcatcaggtc ttgtggagcc gctcgcgatt aagtgaacgc caaaaagggc agggaattac
    23761 aggtgctgat catttctggt ttggtcatac accgttgcgc catcgcgtgg atattggcaa
    23821 cctgcattat attgataccg gcgctgtctt tgggggcgaa ctgactttcg tgcaattgca
    23881 ataattaaaa atcaccatac tcctgtgctg gtcgccagaa accatctata aaatcctcaa
    23941 tcggaaaaca accgccatga cggatccgtt gatcgctcat agaataaaga cactgctgtt
    24001 ccgtgttgta gacatccaca acaatatctt cacaaccgcc atccaggtag caaacaaaaa
    24061 gtaccagcgc gaacatttca tccccgaagt gtggtgccgt accgttaagt ttaggagaga
    24121 ttttacaacg ggggaataac caggacaaat aacccgccat gccgacgggt tctttttgga
    24181 tcaggcaaga cgcataatcc aggcatcaga ttgttgatcg taacgggtac ggtaaaggac
    24241 agagtgttcg ccatttagca gggcagggat actggtttca gcatcccagg catccagttg
    24301 catacataaa tcggggtctg atttacaagg aatggttaac gttcgatcac ccggcgattc
    24361 accgtgtaat tcgccgtcgt cgatttcaaa agcgcctatg cgcacactgg ttttcatctt
    24421 cgtgctctcc atttagctgc cggttgtggt acgaccagtc cgatcgcacc atttttcagc
    24481 ctagttgagg ccgccgaatg tgccagtcaa aaagtgcgct ttttttgtgc gctgtcatgc
    24541 gttactggta aaaagtttcc cgtcccgcac cagttcacgc gggtagctgt ttttcagtcg
    24601 cgaaccaatc cgtttggcta aaccaatcgg ctgatgctgg aaagtaacca acacgtcatc
    24661 cgccactggc gcggcttgcg ggtaaacatc gcgcccgcga taccactcct ccgcttcttg
    24721 cggtgtcagt tcaaaagcgt tctcgttgtc gggggaggca agggcaataa ccgcttcatg
    24781 ctgccagcga taacctttgt tgtgcgtttc ggcaagttta atccccaggc gagaaaatcg
    24841 gactttaccg atcagggctt caatgcctac cggaaacaac cacacttctt tgtcgcgctg
    24901 ccagagtcgc aggtttccat cccagtttaa gccaacactt gcagccgcct gacgaatttg
    24961 tgcggcttcg cgatctttca ccgggctgaa cgggaaattg cccactttgt attttggggt
    25021 gggtaaggcg ggaatggcct gagttttacg cagacgagca acgaagaagc cttcgcagtc
    25081 gtaaatttgt gggaaaacat gcaaaaagcc ttcttcggtc agcgctttgt ttgcaccagg
    25141 gaagagatcg ccaagtggta aaaactctac tgcgtcgggg taagtctctt taagccacag
    25201 gcaaacggat tcgttttctt cccggtttaa ggtacaggtc gagtaaacca gtgtaccgcc
    25261 agagcgtaat gcatgaaagg cactgtcgat cagttcccgt tgggtagctg cgatttcctg
    25321 attgctttct ggtgaccaat tttttagcgc atcgggatct ttacgcacca cgccttcgcc
    25381 ggagcaggga gcgtccagca aaatggcatc gaacatttct ggcaatgccg caccaaacac
    25441 gcggccatca aaatgtgtga gcgcaacatt actgatacca cagcggctga tattggcatg
    25501 taacactttt acccgactgg cggaaaactc attggcaagg attgcccctt cgttattcat
    25561 ccgcgcggca atttgcgtcg ttttggagcc tggcgcagca gcgacatcca tcacccgctg
    25621 tggtgcatta ccgtcagcaa acaaggcggc gacgggcaac attgaactgg cttcctgaat
    25681 ataaaacagg ccacttaaat gctcggcggt actacccaat ggcaatgcat cttcatcgtc
    25741 gcgttcaatc cagaaacctt cttcacacca cggaattggc gtaagcgtcc agccataagg
    25801 agcggttaat cgcaggaaat cagcaacgga ggttttcagc gtattaacgc gaatgctgcg
    25861 gcgcaacggg cgctgacagg cggcaagaaa atcatcaaaa gagagcgtcg aaggcattgc
    25921 ttcgcgcatt tgcgtcagaa aggcgtccgg gaaataaacg gtgtgttggg ccacgagcat
    25981 ataccactgg caaaaaaaac aggcgcgcag tttagcataa acgctccggc gcaggcacgc
    26041 cggagcagtg ggcattattt gggaagcgca gttccccatt cacgccactc tttcggttca
    26101 ctttcctgca acaggaagtg cttgccttcc tgggctttcg gtgccagtgg cgtacccgga
    26161 ggcgtggcga aggcgatgcc gccacggata aactggttaa aggtgccggt tttcactacg
    26221 ccgcccgtca gaccaaagtc cagactgtaa cccgatgcca accagaagac ggaattgtta
    26281 cgcaccaggt gttgatagcg tttactgatg cgcatcgcaa tcatcacgcg atctgacaat
    26341 gtccccagcg tcattccggt aaccgtaccg acttccagac cacggaacag cacaggcgta
    26401 ccgatgccta acgaacaggc ttccggcgct tcaacaataa tgcttaaacc atccaggtaa
    26461 cgcgaatcag taatggtggc ctcttgtaat tcaaagtcgc gacgaggatt gccacggcct
    26521 ggttcgacgt tgatatacgg ctggaggata gtatcaagat gctcaacgcc agctgccgaa
    26581 atttgcggtg tgaccactga gaagcgcgta ccaccgcgag caaaggtctg gacatattcc
    26641 ggatagagca ccgcctttgc ctgtacttca ttgcgcgcgg taatcagatc cagcgtctgg
    26701 atttgcccga tatcaatacc gagatagcga attggcatcc cgaccgccag ttttccggca
    26761 tcgaaagcat gaagcgtaat ctgcccgcca accgcacggg ccgctgtttc ggaagcatac
    26821 agaatacgct tatcgccttt acgctgactg gcgctggcac cgctgaggtt atcgaagcta
    26881 atggctccct ttaatgctct ggagagtggg gatgcctgta cggtcaggcc actaccattc
    26941 agctgaactt tcgccccgcc ttctgcccag aacacgctat tgttggtcag aaggttgcga
    27001 tactccggct taatatgcag atcgatatca aacgcgttag ctcgcggacg cacggtaata
    27061 acttcaccaa cttcaaattt acggtacagc actaccgatc ctgcctgcac atccggcagc
    27121 gtctctgcac tcaaactcac ggttgtggtg ggtaaatcgc taaggctgtt ctccagcgct
    27181 ttttccagat tggcatacag tggatagctg gctttcatct cacctttatc gcccggcaga
    27241 atacgtatcc cgccattgat ccattctgag gcgctggcac cgagaaactc aacgccatcc
    27301 agccccacct tcacatcgac acggctgttg acgacaaatt tgctatcgcc ttttaccagt
    27361 tcgcgatgct gaggctcaat ggcgacggta aaggtaacgc ctttgctggt gagtttacga
    27421 tcaataacct ggcctacctg cacgccgtga agaatgagcg gctgaccagc atcaataccg
    27481 taactttccg gtgctgtcag ggtcagtgtc agaacatcag gttcatgcag cagtgctttt
    27541 tcgcctggca caacaacgaa ctctttgcgt ggctcgccat cgccaggcac caactcaaag
    27601 gtttttccgg tcagcagggc gctgagattg gcatcgctaa gggataattt cgggttgcgt
    27661 aattcgatgc gggtattttc ccgtaacagc gtaacgacgc tgggatcaac ggtcatttct
    27721 ccggtgactt taccaccagg atttaaatcc agtttagtca gctgtccgac ttccagcccc
    27781 tgatacatta acggcgtcga gtcggcggtt aatccggccc cactcggcag ttccagtttt
    27841 attattacgc cacgctggct gtgggccaga tcttcataca gaccaaaggt atcttccgcc
    27901 tcggcaggtt tcgactcttc tggtgaatcg aaggcaatcg caccgttaac cagtgccgcc
    27961 agactttcca gtttcacctt cgcgccactg atactgacgt tggcatcaac gcctgaaacg
    28021 ttccagaaac ggctaccttt tttcaccagg tcggtaaaac gccgctcgat caggacatca
    28081 atcaccacgc cttgtttatt gggattgatg gcatagtcgt agacttttcc caccgggatc
    28141 ttgcggaaat agaccaatga accgctgttc agcgaaccga gatcgggagc ttgcaggtgg
    28201 atcatcagat cgccgttatc cagccgatac ttcggttggg tatcgagcgc gacaaagtga
    28261 tcctgctctt tacctttacc tggcatcatg ccgatatagt taccaccgac gagggcgtcc
    28321 agcccggaga cacctgccaa cgatgctttt ggcgtcacca gccagaactg agtctcttcg
    28381 cgcagcgcat ctttcatatc ggacttgatg ctgaccttga cttcaatctt acgaagatcg
    28441 tcgctgaggc tgatatcctg cactgttccg acttcaacgc cctgataacg aacaggcgta
    28501 cggcccggga caataccatc cgccgacata aagtcgatgg tgacggtatt accccggtcc
    28561 tgataactgt cccaaatcag ccaaccggca atcattagcg cgatgaacgg cagcagccag
    28621 aaaggtgaga tacggcgttt atttttaatc tgcgcttcag tcgtcgaagc gggcgtttcc
    28681 tgactcatgt gcatcccaaa gtaagcggct gtccagccat tccacagcaa gaatagtcaa
    28741 aattaccgct gcgccgaaat aaaacgcagc cggtcccata gtaaaagcga ggatctgatc
    28801 gcgattaatc agcgacatgg ttaaagatat gacaaacagg tcgagcatcg accagcgacc
    28861 aatccaggtc accatccgca gtaacagaat gcgtgtgcgt aaaccttgct ggcatttaaa
    28921 atgaatgctc aacagtaaag tgaacatgac gatcacttta gtaaacggta ccagaatact
    28981 ggcgataaac acgattcctg caaccgcaat gttgctactt gccagcgaca taattccgga
    29041 aagaattgta tcttcctgcc gtcctccgtt cagataaatg atagaaatag gcaacaggtt
    29101 ggcaggtaac aataaaacga ttgacgctaa cagcgccgcc cagcattttt gcagactatg
    29161 acggcgacgc aggcgtagcg gaatatgaca acgcgggcag cgaccacgct gatcaggata
    29221 gccggtaaaa tggcacccaa gacagacacg aagtttctcg tccctacgcg tagcggggcg
    29281 ctgcggataa aatcgctccc acagctcctc gacattaaga tgtgacaacg tcaccgtcgt
    29341 taaaatcacc aacgccacaa aagagaacaa gccgacaccc gcctggatat gggcataatc
    29401 ctgtaccttt atagaagcaa cgccaatgcc gaccaggtag atatcgagca ttacccactc
    29461 tttaagtcgc tcaagcatca gcagtaccgg gcgtagattc attcccagtc ggttaccaaa
    29521 ccataaataa gctatggagg tcaccagaat gagcggggca ccaataacgc agaaaaagac
    29581 catcgacccc gttatcgcat cgccctgttt ggtcatttgc cagatgcctt gcataacgtt
    29641 ggcgtcaata cgaatgccta acagccagat atgcaacagc ggttcgcccc aggcaaacgg
    29701 catcagcaac agcatggtga aggccattgc cgccaggcgc gttagcgacc agtcgcgccc
    29761 gtcacgaatt tttgcctgac agcgcggaca ataggcactt tgatgagaat ttatctcggg
    29821 caggctaaac agcatgtcac attgcgggca acgttggtaa tcaccacgcg gcagttcctc
    29881 gccgattgcc ctcactgtta tcttttttgt cggcgtaatt tgtggtgtgt taagagccat
    29941 gacgtgccaa atgtaataag aattaacatt aatcttaacc catgatggga atgatcttga
    30001 gcattaacgc atttaatgct tattttaata ggctggcagt tccaggtaag acaaccaagt
    30061 gattatataa tgaacaaaac agaattttac gcggatttaa atcgcgactt taacgcgctg
    30121 atggcgggag aaaccagttt tctggcaacg cttgcgaaca ccagtgcgtt gttatatgag
    30181 cgtctcactg acgtaaactg ggcaggtttt tatttgcttg aggacgatac actggtgctc
    30241 ggaccatttc agggcaaaat tgcctgtgtc cggatacccg tcgggcgtgg cgtgtgcggc
    30301 actgcggttg cccgcaatca agtgcagcgt atcgaggatg ttcatgcgtt tgacgggcat
    30361 attgcctgtg atgcggcgag taattctgaa attgttctgc cgctggtggt gaaaaatcag
    30421 attattggtg ttctcgacat cgatagtacc gtcttcggtc gctttacaga cgaggacgag
    30481 caaggcttac gtcagcttgt ggcacagctt gaaaaagtgc ttgcaacgac ggattacaaa
    30541 aaattctttg cgagcgtcgc aggataatca acggataacg tagcatttac tgatagcgtc
    30601 attataatga cgcctgttca tgcctgcgct tgttggctac gtccgttgta atcaggaaat
    30661 ttcatggaaa atcaacctaa gttgaatagc agtaaagaag taatcgcgtt tctggccgaa
    30721 cgttttcccc actgtttcag tgcggaaggt gaagcgcgtc cgctgaaaat cggtattttt
    30781 caggatttgg tcgatcgtgt tgccggggaa atgaacctga gcaaaacgca attacgatcc
    30841 gctttacgtc tctacacttc gagctggcgt tacctttacg gtgttaaacc cggcgcaacg
    30901 cgtgtcgatc ttgacggcaa cccatgcggt gagctggacg agcaacatgt agagcatgct
    30961 cgcaagcagc ttgaagaagc gaaagcgcgt gttcaggcac agcgtgctga acagcaagcg
    31021 aaaaaacgcg aagctgccgc agctgctggt gagaaagaag acgcaccgcg tcgcgaacgc
    31081 aagccacgtc cgaccacgcc acgccgcaaa gaaggcgctg aacgtaaacc tcgtgcgcaa
    31141 aagccggtag agaaagcgcc aaaaacagca aaagcacctc gcgaagaaca gcacacccca
    31201 gtttctgaca tttcagctct gactgtcgga caagccctga aggtgaaagc gggtcaaaac
    31261 gcgatggatg ccaccgtatt agaaatcacc aaagacggcg tccgcgtcca gctgaattcg
    31321 ggtatgtctt tgattgtgcg cgcagaacac ctggtgttct gaaacggagg ccgggccagg
    31381 catgaacatg ttttttaggc ttaccgcgtt agctggcctg cttgcaatag caggccagac
    31441 cttcgctgta gaagatatca cgcgtgctga tcaaattccg gtattaaagg aagagacgca
    31501 gcatgcgacg gtgagtgagc gcgtaacgtc gcgcttcacc cgttctcatt atcgccagtt
    31561 cgacctcgat caggcatttt cggccaaaat ctttgaccgc tacctgaatc tgctcgatta
    31621 cagccacaac gtgctgctgg caagcgatgt tgaacagttc gcgaaaaaga aaaccgagtt
    31681 aggcgatgaa ctgcgttcag gcaaactcga cgttttctac gatctctaca atctggcgca
    31741 aaagcgtcgt tttgaacgtt accaatacgc tttgtcggta ctggaaaagc cgatggattt
    31801 caccggcaac gacacttata accttgaccg cagcaaagcg ccctggccga aaaacgaggc
    31861 tgagttgaac gcgctgtggg acagtaaagt caaattcgac gagttaagcc tgaagctggc
    31921 aggaaaaacg gataaagaaa ttcgtgaaac cctgactcgc cgctacaaat ttgccattcg
    31981 tcgtctggcg caaaccaaca gcgaagatgt tttctcgctg gcaatgacgg cgtttgcgcg
    32041 tgaaatcgac ccgcatacca actatctttc cccgcgtaat accgaacagt tcaacactga
    32101 aatgagtttg tcgctggaag gtattggcgc agtgctgcaa atggatgatg actacaccgt
    32161 tatcaattcg atggtggcag gtggtccggc agcgaagagt aaagctatca gcgttggtga
    32221 caaaattgtc ggtgttggtc aaacaggcaa gccgatggtt gacgtgattg gctggcgtct
    32281 tgatgatgtg gttgccttaa ttaaagggcc gaagggcagt aaagttcgtc tggaaatttt
    32341 acctgctggt aaagggacca agacccgcac tgtaacattg acccgtgaac gtattcgtct
    32401 cgaagaccgc gcggttaaaa tgtcggtgaa gaccgtcggt aaagagaaag tcggcgtgct
    32461 ggatattcct ggcttctatg tgggtttgac agacgatgtc aaagtgcaac tgcagaaact
    32521 ggaaaaacag aatgtcagca gtgtgatcat cgatctgcgt agcaatggtg gtggggcgct
    32581 gaccgaagcg gtatcgctct caggtctgtt tattcctgcg ggtcctattg ttcaggtccg
    32641 tgataacaac ggtaaagttc gtgaagacag cgataccgac ggacaggttt tctataaagg
    32701 cccgctggtg gtactggttg accgcttcag tgcttcggct tcagaaatct ttgccgcggc
    32761 aatgcaggat tacggtcgtg cactggttgt gggtgaaccg acgtttggta aaggcaccgt
    32821 tcagcaatac cgttcattga accgtattta cgatcagatg ttacgtcctg aatggccagc
    32881 gctgggttct gtgcagtaca cgatccagaa attctatcgc gttaacggcg gcagtacgca
    32941 acgtaaaggc gtaacgccag acatcatcat gccgacgggt aatgaagaaa cggaaacggg
    33001 tgagaaattc gaagataacg cgctgccgtg ggatagcatt gatgccgcga cttatgtgaa
    33061 atcaggcgat ttaacggcct ttgaaccgga gctactgaag gaacataatg cgcgtatcgc
    33121 gaaagatcct gagttccaga acatcatgaa ggatatcgcg cgcttcaacg ctatgaagga
    33181 caagcgcaat atcgtttctc tgaattacgc tgtgcgtgag aaagagaata atgaagatga
    33241 tgcgacgcgt ttggcgcgtt tgaacgaacg ctttaaacgc gaaggtaaac cggagttgaa
    33301 gaaactggat gatctaccga aagattacca ggagccggat ccttatctgg atgagacggt
    33361 gaatatcgca ctcgatctgg cgaagcttga aaaagccaga cccgcggaac aacccgcacc
    33421 cgtcaagtaa tatcaatcag gcacaagaaa ttgtgcctga ttttttaaca gcggcaagat
    33481 gccgtaaatc agatgctaca aaatgtaaag ttgtgtcttt ctggtgactt acgcactatc
    33541 cagacttgaa aatagtcgcg taacccatac gatgtgggta tcgcatattg cgttttgtta
    33601 aactgaggta aaaagaaaat tatgatgcga atcgcgctct tcctgctaac gaacctggcc
    33661 gtaatggtcg ttttcgggct ggtactgagc ctgacaggga tacagtcgag cagcgttcag
    33721 gggctgatga tcatggcctt gctgttcggt tttggtggtt ccttcgtttc gcttctgatg
    33781 tccaaatgga tggcattacg atctgttggc ggggaagtga tcgagcaacc gcgtaacgaa
    33841 agggaacgtt ggctggtcaa tactgtagca acccaggctc gtcaggcggg gatcgctatg
    33901 ccgcaagtgg ctatctacca tgcgccggac atcaacgctt ttgcaaccgg tgcgcgccgt
    33961 gatgcctctc tggttgctgt cagcaccggt ttgctgcaga acatgagccc ggatgaagcc
    34021 gaggcggtaa ttgctcacga aatcagccac atcgccaatg gtgatatggt caccatgacg
    34081 ctgattcagg gcgtggtgaa caccttcgtt atctttattt cccgtatcct ggcgcagctt
    34141 gccgcgggtt ttatgggcgg aaatcgtgat gaaggtgaag agagcaacgg caacccgctg
    34201 atctactttg cggttgcaac ggttctggaa ctggtgtttg gtattctggc gagcattatc
    34261 accatgtggt tctcgcgtca tcgtgaattc catgctgatg ccggttcggc aaaactggtt
    34321 ggtcgcgaga aaatgattgc cgcgctgcag cgcctgaaaa ccagctatga accgcaagaa
    34381 gcaaccagca tgatggcttt ctgcattaac ggtaagtcga aatcgctcag tgagttgttt
    34441 atgactcacc cgccgctgga taaacgtatt gaagctctgc gtacgggtga atacctgaag
    34501 taatatgaag taaatactaa aaagcgcgtc ctgctcgacg cgcttttttt atgccctgga
    34561 tcttggctga gtgatacgta acccactgat acaggcagca atcactgcca gaatagccgc
    34621 agccatcagc gagacgtgtg taccattatc tccaaactga tttagcatca gcgccaccag
    34681 cgccgcgccg ctactctgac ccagtagacg agccgttcct aacatgccac tggctccacc
    34741 gctacgttcg cgaggcgcgg aggtaataat ggtgtggtta ttgggtgact ggaacaagcc
    34801 aaatccagcg ccacataaga tcatcggcca gataatattg atatccgcag gtgacgcggg
    34861 cagcagaacc agggaaaaaa gccccgcagc catgatgaat aatcctaaag cacccagcaa
    34921 tcctgcatgt acgcgttcaa tcaaatagcc tgccaacgga gccatcacca tcgttgctaa
    34981 cggccacggt gtcagaagta aacctgtttc gacttcacta cgcccgagca cggtttgcag
    35041 gtaaaagggc agggaaacca ttgccagcat ttgtgcgcag aaagagcaaa cagatgtgca
    35101 aatagaaagt gaaaacagcg ggatacgcag taaatccacc ggtagcagcg gtactggaag
    35161 agaaagctgg cggcgaataa agaaaatacc aacgacaacc attacaacca gttccgcagc
    35221 aattaacgtc agcgattgcc cctgagcgaa accactcaac gcagtgataa gcaggccgaa
    35281 ggttaacgcg ttcatcacgg cgctgggcag gtcgaaacgg ggtttactgg cgcgagaacc
    35341 attgggtggc agaaaacgca tcgccagaag cagggcgata atacccaacg gtacgttgat
    35401 taaaaataac catttccagg atgcgatgga gaggattgct gcagcaattg tcggcccggc
    35461 agcagaagag acggcaacaa taaacgagtt tatgcccatc cctctaccca gaaaacgttg
    35521 tggatagatc aggcggataa gggcggtatt aacgctcatc aacgctgcac cgccgaaacc
    35581 ttgtatgaca cgcgcaaggg tgagtatttg cagcgaatca gaaagggcgc agaacagtga
    35641 agacaacaga aaaacgacca gaccgcattt ataaatacgt cgatagccaa acatatcacc
    35701 cagaaacgaa aacgagagca gggagatgac aatggcgatt tgataggcgt tcactaccca
    35761 gatggaactg gctggcgtgg catgaaggtc cgtggcgatt gttggcaggg cgacgttggc
    35821 gattgcgccg tcaaggacag ccatcgaaat accaatcaca atggttaata tcgcaccgta
    35881 tcgctggggc aatggcaggc cgtcggcctg aacttttggc ataggaattt taaatctttg
    35941 gtgaataatc aacaggggta tgattctagc actgatattc acggtggatg tcgcagaatt
    36001 gtaactaacc ggcaaaagat ggattgcgac caccctggtg cgaaattata ataaaaaaca
    36061 gttctgattt ttataaaaca ctcgcaatga ggtgataaat ggctaacgca gatctggata
    36121 aacagcctga ttctgtatct tccgtgctaa aagtttttgg cattttgcag gcgctgggtg
    36181 aagagcgcga aatagggata accgagctgt cgcagcgcgt catgatgtca aaaagcaccg
    36241 tttatcgctt tttacagacc atgaaaacct taggttatgt ggcgcaggaa ggggagtcgg
    36301 agaaatattc cctgaccctg aaattgtttg aactgggcgc tcgcgcgtta caaaacgtcg
    36361 atttaattcg tagcgcagat atccagatgc gtgagatctc ccgcctgacc aaagaaacta
    36421 tccacctcgg cgcactggac gaagacagta ttgtttacat tcacaaaatt gactctatgt
    36481 acaatttgcg catgtattca cggattgggc gtcgtaatcc actgtacagc accgcgattg
    36541 gtaaggtact gctggcatgg cgcgatcgcg atgaagtgaa gcaaattctt gagggcgtgg
    36601 agtataaacg cagtaccgag cggaccatca ccagtacaga agcgttatta ccggttctgg
    36661 accaggtgcg cgagcagggg tatggcgaag ataacgaaga gcaggaagaa gggctgcgat
    36721 gcattgcggt tccggtattt gatcgctttg gcgtggtcat tgccggtttg agcatctcct
    36781 tcccgacgtt gcgtttctct gaagagcgtt tacaggaata tgtcgcaatg ttgcataccg
    36841 cagcgcgcaa aatttctgcc caaatgggtt atcacgacta tccgttctga tgagagtaag
    36901 aacctgtcgg aatatcaaac agacaggttc tttatttagc atgagaaaaa taaagttgaa
    36961 ggtggcgtta gattaaacgc agttgatata agaatatttt actcatgagt gagaatctgg
    37021 ttatttatta cacttaacca ttatcgacca cgatattgct tttgcgtaac agcgggcaat
    37081 ctgttatccc caaaaaacca cttttagtgt gcaagtattg taccgtgctg gtgcctctgg
    37141 cagtcagata ggtacattgc aaacctaatt ctgcggcatt ctctttgctt ccaatcaaaa
    37201 cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata actgtgcgaa
    37261 tgatgaatcg cattacaacc tcttctcttt tttatgttcg cttaatcgta gcggcaatat
    37321 gcgctgaagc aagcgactca ttccgaaaaa gcacgaatat cgacatagtt agacgctgtt
    37381 taactaacgc atggtagttt aatgacataa ggtaggtgaa acggagattg gagtgaaaaa
    37441 gtttcgatgg gtcgttttgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt
    37501 attcaacatg atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca
    37561 gtttatcccg tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt
    37621 agaatagcgc ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg
    37681 aacattgcgt ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt
    37741 tttattaatc gtgccagttc tcggactaac gaacagattg aactgcttga ggcgttgctg
    37801 gatcagcaaa aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag
    37861 aaagcagata aaaagactat tgagagtcaa aaatcggttg aagacgaaga tattattcgc
    37921 ctggtcgccg aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact
    37981 caattcatcc tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc
    38041 tgacttcaaa taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt
    38101 accccattaa tttatt
//
LOCUS       DAZKIF010000029        37947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZKIF010000029 DAZKIF010000000
VERSION     DAZKIF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.029"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(150..845)
                     /gene="aqpZ"
                     /locus_tag="ACHOTW_001548"
     CDS             complement(150..845)
                     /gene="aqpZ"
                     /locus_tag="ACHOTW_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP9843679.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1271..2929
                     /gene="ybjD"
                     /locus_tag="ACHOTW_001549"
     CDS             1271..2929
                     /gene="ybjD"
                     /locus_tag="ACHOTW_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP9843680.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2926..3882)
                     /gene="ybjX"
                     /locus_tag="ACHOTW_001550"
     CDS             complement(2926..3882)
                     /gene="ybjX"
                     /locus_tag="ACHOTW_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP9843681.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4033..5148
                     /gene="macA"
                     /locus_tag="ACHOTW_001551"
     CDS             4033..5148
                     /gene="macA"
                     /locus_tag="ACHOTW_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP9843682.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5145..7091
                     /gene="macB"
                     /locus_tag="ACHOTW_001552"
     CDS             5145..7091
                     /gene="macB"
                     /locus_tag="ACHOTW_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP9843683.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7164..7388)
                     /gene="cspD"
                     /locus_tag="ACHOTW_001553"
     CDS             complement(7164..7388)
                     /gene="cspD"
                     /locus_tag="ACHOTW_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP9843684.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7538..7612
                     /gene="yljB"
                     /locus_tag="ACHOTW_001554"
     CDS             7538..7612
                     /gene="yljB"
                     /locus_tag="ACHOTW_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP9843685.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7711..8031
                     /gene="clpS"
                     /locus_tag="ACHOTW_001555"
     CDS             7711..8031
                     /gene="clpS"
                     /locus_tag="ACHOTW_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP9843686.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8062..10338
                     /gene="clpA"
                     /locus_tag="ACHOTW_001556"
     CDS             8062..10338
                     /gene="clpA"
                     /locus_tag="ACHOTW_001556"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP9843687.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10684..10771)
                     /locus_tag="ACHOTW_001557"
     tRNA            complement(10684..10771)
                     /locus_tag="ACHOTW_001557"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10735..10737),aa:Ser,seq:gga)
     gene            complement(11025..11243)
                     /gene="infA"
                     /locus_tag="ACHOTW_001558"
     CDS             complement(11025..11243)
                     /gene="infA"
                     /locus_tag="ACHOTW_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9843688.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11528..12232)
                     /gene="aat"
                     /locus_tag="ACHOTW_001559"
     CDS             complement(11528..12232)
                     /gene="aat"
                     /locus_tag="ACHOTW_001559"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP9843689.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12274..13995)
                     /gene="cydC"
                     /locus_tag="ACHOTW_001560"
     CDS             complement(12274..13995)
                     /gene="cydC"
                     /locus_tag="ACHOTW_001560"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP9843690.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     PLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13996..15762)
                     /gene="cydD"
                     /locus_tag="ACHOTW_001561"
     CDS             complement(13996..15762)
                     /gene="cydD"
                     /locus_tag="ACHOTW_001561"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP9843691.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15885..16850)
                     /gene="trxB"
                     /locus_tag="ACHOTW_001562"
     CDS             complement(15885..16850)
                     /gene="trxB"
                     /locus_tag="ACHOTW_001562"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9843692.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17395..17889
                     /gene="lrp"
                     /locus_tag="ACHOTW_001563"
     CDS             17395..17889
                     /gene="lrp"
                     /locus_tag="ACHOTW_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP9843693.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18024..22052
                     /gene="ftsK"
                     /locus_tag="ACHOTW_001564"
     CDS             18024..22052
                     /gene="ftsK"
                     /locus_tag="ACHOTW_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9843694.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            22207..22818
                     /gene="lolA"
                     /locus_tag="ACHOTW_001565"
     CDS             22207..22818
                     /gene="lolA"
                     /locus_tag="ACHOTW_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP9843695.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22829..24172
                     /gene="rarA"
                     /locus_tag="ACHOTW_001566"
                     /gene_synonym="mgsA"
     CDS             22829..24172
                     /gene="rarA"
                     /locus_tag="ACHOTW_001566"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP9843696.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24263..25555
                     /gene="serS"
                     /locus_tag="ACHOTW_001567"
     CDS             24263..25555
                     /gene="serS"
                     /locus_tag="ACHOTW_001567"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9843697.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25794..28238
                     /gene="dmsA"
                     /locus_tag="ACHOTW_001568"
     CDS             25794..28238
                     /gene="dmsA"
                     /locus_tag="ACHOTW_001568"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP9843698.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28249..28866
                     /gene="dmsB"
                     /locus_tag="ACHOTW_001569"
     CDS             28249..28866
                     /gene="dmsB"
                     /locus_tag="ACHOTW_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP9843699.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28868..29731
                     /gene="dmsC"
                     /locus_tag="ACHOTW_001570"
     CDS             28868..29731
                     /gene="dmsC"
                     /locus_tag="ACHOTW_001570"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP9843700.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29767..30393)
                     /gene="ycaC"
                     /locus_tag="ACHOTW_001571"
     CDS             complement(29767..30393)
                     /gene="ycaC"
                     /locus_tag="ACHOTW_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP9843701.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30708..31856
                     /gene="ycaD"
                     /locus_tag="ACHOTW_001572"
     CDS             30708..31856
                     /gene="ycaD"
                     /locus_tag="ACHOTW_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9843702.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32066..33496
                     /gene="ycaM"
                     /locus_tag="ACHOTW_001573"
     CDS             32066..33496
                     /gene="ycaM"
                     /locus_tag="ACHOTW_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9843703.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33497..33607)
                     /locus_tag="ACHOTW_001574"
                     /pseudo
     CDS             complement(33497..33607)
                     /locus_tag="ACHOTW_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(33706..34446)
                     /gene="pflA"
                     /locus_tag="ACHOTW_001575"
     CDS             complement(33706..34446)
                     /gene="pflA"
                     /locus_tag="ACHOTW_001575"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP9843704.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34638..36920)
                     /gene="pflB"
                     /locus_tag="ACHOTW_001576"
     CDS             complement(34638..36920)
                     /gene="pflB"
                     /locus_tag="ACHOTW_001576"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9843705.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(36975..37832)
                     /gene="focA"
                     /locus_tag="ACHOTW_001577"
     CDS             complement(36975..37832)
                     /gene="focA"
                     /locus_tag="ACHOTW_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP9843706.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9379 a         9307 c        10069 g         9192 t
ORIGIN      
        1 aacgtcctgt aagacgcgcc ctcagcccac atccatgtgg gctgccccgg ccttcgggga
       61 acgcttcagc gattttgacg ccaccaatac ccgtgcggtt tgtaatgtcg aagataaaac
      121 aaccagggca gataaaatgc caatgctaat taattacgct tttccagcag agtccggtaa
      181 atcagaccac cgataatgcc accgacaatt ggcaccaccc agaagaacca cagttgttct
      241 aatgcccagc cgccctggaa gatggcaacc gcggtgctgc gcgccgggtt aacagaagtg
      301 ttagtcaccg gaatactgat taagtgaatc agggttaagg ccagaccaat agcgatcggc
      361 gcaaaacctg ccggagcgaa tttgtcggtt gcaccgtgga tcactaacag gaagcccgca
      421 ctcaatacca gttcaactac cagcgcggaa agcatggaat aaccgcctgg tgaatgctcg
      481 ccataaccgt tagaagcaaa accgctggct gccgcgtcaa aacccgtttt accactggca
      541 attaaataca gcagcgctgc cgcaacaata ccgccgacaa cctgggcaat tacgtagcca
      601 acgacttctt ttgccggaaa acgtccgcca gcccataaac caatagtgac cgccgggtta
      661 aaatgaccac cagaaatatg accaacagca aaggccatcg tcagaacggt cagaccgaac
      721 gccaacgcca cgccggcaaa accaatgcct aattccggga agcctgcggc cagtacagca
      781 ctaccacagc caccaaaaac aagccagaaa gtaccaaaac attcagctgc taattttctg
      841 aacatatcca cctcaattaa aaattgaccc tgtgaaaaat atggtcgttt tatagggtcg
      901 tcgtaaaaaa gtgacgacgg aaataatgcg cggctatttt aaaaacgaag gcgagtcatt
      961 caccagataa ataaatccag taaatttgat ttagggcaac agcgggttgc cccatatagt
     1021 catttgtctg attgacagtg tagtgcaagc aaaagattta atcctttagg cgtaataaaa
     1081 aataatttat catgctaatt atttgatttt gttgtttttg cagacttatc agcaagaggg
     1141 agtataacgc gattattcgc tcatttttca gacatttgcc atgcttaaat gtgatgtcat
     1201 cacgtattag caaggccttt cccgttatac tgccagcgta aaggataagt cacatatttc
     1261 tggaggggat atgattcttg agcgcgttga aattgtgggt tttcgcggta tcaaccgttt
     1321 gtcgttgatg ctggaacaaa acaacgtcct gattggggag aacgcgtggg gtaaatccag
     1381 cttgctggac gccttaaccc tgctgctatc gccagaatca gatctctacc attttgagcg
     1441 cgacgatttc tggttcccgc cgggagatat caacgggcga gaacatcatc tgcatattat
     1501 tttgaccttc cgcgaatcgc tgccaggtcg acatcgggtt cgccgttatc ggccgctgga
     1561 agcgtgctgg acgccatgca ccgatggcta tcaccgtatt ttttatcgtc tggaagggga
     1621 gagtgcggaa gacggcagcg tgatgacact gcgcagtttt ctcgataaag acggacatcc
     1681 gattgatgtt gaggatatta acgatcaggc acaccatctg gtgcgtttaa tgccggtgct
     1741 gcgcttgcgt gatgcccgtt ttatgcgccg tattcgtaac ggcacggtgc caaatgtccc
     1801 caatgtggaa gtcaccgcgc gccagctcga tttcctcgcc cgtgagttgt cctcacatcc
     1861 gcaaaatctc tctgatggac agattcgtca gggactttcc gcaatggtgc agttgcttga
     1921 gcattatttc tctgagcagg gggctggaca ggcgcgatat cgtttaatgc ggcggcgggc
     1981 cagcaatgag caacgaagct ggcgctatct ggacatcatc aaccggatga ttgaccgacc
     2041 tggagggcgc tcgtatcggg ttattttgct cggcctgttt gctactttat tgcaggcaaa
     2101 aggcacattg cgactggata aagacgcccg tccattgttg ctgatcgaag atccagaaac
     2161 gcgtttacac cccattatgc tttcagttgc ctggcatctg ttgaatcttt tgcctttaca
     2221 acgtattgct accaccaact ctggcgagct gctttcgtta actccagtgg aacatgtttg
     2281 ccgcctggtg cgtgaatctt ctcgtgttgc cgcctggcgt ctggggccaa gtggcttgag
     2341 caccgaagac agccgccgca tctcttttca cattcgtttt aatcgtccgt catcgctgtt
     2401 tgctcgctgc tggttgctgg tggaagggga aacggaaacc tgggttatca atgaactggc
     2461 gcgtcagtgc ggacatcatt ttgatgccga agggatcaag gtcattgagt ttgcccagtc
     2521 cgggctaaag ccactggtta aatttgcccg ccgaatgggg attgaatggc atgtactggt
     2581 cgatggcgat gaagcaggga agaaatatgc cgctacggta cgcagcctgt tgaataatga
     2641 tcgggaagcc gaacgagaac atttaacggc gttaccggcg ctggatatgg aacattttat
     2701 gtatcgccag ggattttccg atgtgttcca ccgcgtggcg caaatcccgg aaaatgtccc
     2761 gatgaatctg cgcaaaatta tctcgaaagc gatccatcgc tcttccaaac ccgatcttgc
     2821 cattgaagtg gcaatggagg ccggacgtcg tggtgtggac tccgtaccga cgctgctgaa
     2881 aaaaatgttc tcacgcgtgc tgtggctggc gcgcggtcgc gcggattaac cgcgaaacat
     2941 cgtggccatt tgtggctgaa tagcgtcgag catctcatag cgccgacggt attcagcccg
     3001 ttttttactg gcgatttcgg caatctcttt tcgtgctatc tgtgctggaa ggcggtaatg
     3061 gcgttcagca tcacatacgc cgccaaccga ttcccagaaa gcgttgtaat cggcatggat
     3121 cttgccttct ttatcgcgat aacgcaggct gcggtaaata tgcgtttcat tgctgacggc
     3181 aataatctgc tctacctgca aacgttgggc aaacagacag gccgcttcca tcacgaggcg
     3241 tttgggaaat agcccgtggc aggctttcgt cgcattctgg atttcctgat gtggaatttc
     3301 ccattttgcg ccttgcagtc caccaataaa catcgttctt ttcccctgat attcacacag
     3361 ggtaaacgtg atttctgcca gaggaatacc ttcgctgttg cggaacagga ttgtgctgtc
     3421 accttcttta tccattgaga tcatcatggt cagctcaagc gtgaactgct cgccgttttt
     3481 gccttccagc ttcgccagtt gcagcccggg ggtattcaaa tataagctga attcttccgc
     3541 cgacatacat ccacggagta acgcataatg gtaacgtaac gcctccagca attgcttacg
     3601 gctaagattc gccgcaaggt aagggcgatg cagacgcaca ggcagtcgcg gctggcgcgt
     3661 taacaataca ttgagattag gccagtggga aagttcgttc atccactcaa cgcttaaacg
     3721 cggcataatc aacgagcgca gcaaaaattt ctggcgaaaa ctacggcgat gccagaattt
     3781 acccggccga cactgtccac gtgccagact aagaaaaagt gacaggctgc tgagagattc
     3841 agatgacgta aaggtccgtt cggttagctg cgacatattc atgaaatcaa tggttgtaca
     3901 tgacgtcgat ttcaccattg cgtatcttaa ccaaacatca atagtgtgat tactaacgta
     3961 aattttaggg ttttgttgat atttcgttga agttaatgac ccggattggc atatggagta
     4021 ttcagaatat ttatgaaaaa gcggaaaacc gtgaagaagc gttacgttat tgcgctggtg
     4081 atagtcatcg ccggactgat tacgttatgg agaattctta acgcacccgt gccgacttat
     4141 cagacactga ttgtgcgccc cggtgattta cagcaaagcg tgctggcgac cggaaagctg
     4201 gacgcgctgc gtaaggttga cgtgggcgcg caggtcagcg gtcagttgaa aacgctgtcg
     4261 gtggcgattg gcgataaagt aaaaaaagac cagcttttag gggttattga tcctgaacag
     4321 gctgaaaacc agatcaagga ggtcgaagca acgctgatgg agctacgcgc gcagcggcag
     4381 caggcggaag cggagctgaa actggcgcgg gtgacgtatt cccgtcagca acgtctggca
     4441 caaacgcagg cggtttcact gcaggatctc gacaccgccg cgacggagat ggctgtgaaa
     4501 caggcgcaaa ttggcaccat tgacgcgcaa atcaagcgca atcaggcttc tctcgatacg
     4561 gctaaaacca atctcgatta cactcgcatc gttgccccga tggccgggga agtcacgcaa
     4621 atcaccactc tgcaaggcca gacggtgatt gccgcacaac aagcaccgaa cattctgacg
     4681 ctggcagata tgagcaccat gctggtaaaa gcgcaggttt ctgaagcgga tgtaatccac
     4741 ctgaagccgg ggcaaaaagc ctggtttacg gtacttggcg atccactgac gcgctacgag
     4801 gggcaaatca aggatgtact accgacgccg gaaaaggtta acgacgctat tttctattac
     4861 gcccgttttg aagtccccaa ccccaatggt ttgctgcggc tggatatgac tgcgcaagtg
     4921 catattcagc tcaccgatgt gaaaaatgtg ctgacgatcc ctctgtcggc gttaggcgat
     4981 ccggttggcg ataatcgtta taaagtcaaa ttgttgcgta atggtgaaac acgcgagcgt
     5041 gaagtgacga ttggcgcacg taacgatacc gatgttgaga ttgtcaaagg gctggaagcg
     5101 ggcgatgaag tggtgattgg tgaggccaaa ccaggagctg cacaatgacg cctttgctcg
     5161 aattaaagga tattcgtcgc agctatcctg ccggtgatga gcaggttgag gtgctgaagg
     5221 gcatcaccct cgatatttat gccggtgaga tggtagcgat tgttggcgct tcgggttccg
     5281 gtaaatcgac cctgatgaat attctcggct gtctggataa ggccaccagc ggcacctatc
     5341 gcgtcgccgg tcaggatgtt gccacgctgg acgccgatgc gctggcgcaa ctgcgccgcg
     5401 agcatttcgg ctttattttc cagcgttacc atttgctttc gcatttaacc gccgagcaga
     5461 acgttgaagt accagccgtc tatgctggtc ttgagcggaa acagcggctg cttcgcgccc
     5521 aggagttgct gcaacgactg gggctggaag accgtacaga gtattatccg gcacagcttt
     5581 cgggtggtca gcaacagcgc gttagcatcg cgcgggcatt gatgaacggc ggtcaggtaa
     5641 ttcttgccga tgaaccgacc ggcgcactgg acagccattc aggcgaagag gtgatggcga
     5701 tcctgcatca gctgcgcgat cgcgggcata cggtgattat cgtcactcac gatccgcagg
     5761 tcgctgctca ggccgagcgg gtgatcgaaa ttcgcgacgg cgaaattgtg cgcaatcctc
     5821 ccgccattga aaaagtgaat gttgctggcg ggacggaacc tgttgtcaac acggcgtctg
     5881 gctggcggca gtttgtcagc ggttttaatg aggcgctgac gatggcatgg cgggcgctgg
     5941 cagcgaataa aatgcgtact ttactgacca tgctggggat tataatcggt attgcgtcgg
     6001 tggtttccat tgtcgtggtg ggcgacgccg ccaaacaaat ggtgctggcg gatattcgtt
     6061 ctattggtac gaatactatt gatgtctatc ccgggaaaga ttttggcgat gacgatccgc
     6121 aatatcaaca ggcgctgaag tacgatgact taatcgccat ccaaaaacaa ccgtgggtcg
     6181 cttcagccac acctgccgtc tcgcaaaacc tgcgcctgcg ttataacaat gttgatgttg
     6241 ctgccagtgc caatggcgtg agcggcgatt attttaatgt ctatggcatg accttcagtg
     6301 aaggaaacac ctttaaccag gaacagctga acggtcgtgc gcaggtcgtg gttctcgaca
     6361 gtaatactcg ccgccagctt ttcccccata aagcagatgt ggttggcgag gtgattctgg
     6421 tcggcaatat gcccgccaga gtcattggtg tggcggaaga aaaacagtcg atgtttggta
     6481 gcagtaaagt gctgcgtgtc tggctacctt acagcacgat gtccgggcga gttatgggcc
     6541 agtcgtggct gaactccatt actgtcaggg tgaaagaagg atttgacagc gccgaggcgg
     6601 aacagcaact cacgcgttta ctttcactgc gccacggtaa gaaggatttc tttacctgga
     6661 acatggacgg cgtcttgaaa accgttgaaa agaccacacg tactttacaa ctgtttatga
     6721 cgctggtggc ggtgatttcg ctggtagtgg gcggtattgg tgtaatgaat attatgctgg
     6781 tgtcagtgac cgagcggacg cgggaaattg gcattcgcat ggctgtaggt gcgcgagcaa
     6841 gcgatgtttt gcaacagttc ctgatcgaag ccgtactggt ttgcctggtc ggtggcgcgt
     6901 tgggaataac actgtcactg ttaattgctt tcaccttgca gcttttctta cccggctggg
     6961 agattggttt ttcaccgttg gcactgctgc tggcgtttct ctgctcgacg gtcaccggga
     7021 ttttatttgg ctggttaccc gcacgaaatg cggcacgact ggatccagta gatgctctgg
     7081 cacgagagta atttttgaga taaaaatgcc agccgatcgg gctggcattt tgcctttagg
     7141 atgtacacaa tgagacagaa gagctatgcg actgccgctt ctacttcgac gggcacaata
     7201 acactggcgt gattgccttt tggcccctgg tggacatcaa actgaacgga ttgtccagct
     7261 tttagcgttc tgtaaccatc catctgaatg gtggaataat gagcgaaaat atcttcgccg
     7321 ccgccttcag ggcagatgaa accaaaccct ttggcattgt tgaaccactt aacagtaccc
     7381 ttttccatgc ttcgacatcc ttcgcaaatc ttatacaagt aagatggaat aaaccggggt
     7441 cagagagggg gctgttcaaa acctcgccaa ctctagaaat acaatttaga gaattagggc
     7501 gagccgtcaa gcatttgaca ggggacaggg ggcaggtatg aatcaaaaat ttgaagcagt
     7561 taacgctatt gacaggaatg tgacagatgt cgctgatgcc aacgatagat gatagttatc
     7621 tatcatgtgg agtagattgg tcaggcaaat aagctcttgt cagcggcagg gcgttctgcc
     7681 gataaccgta accgaagatg ataactgaca atgggtaaaa cgaacgactg gctggacttt
     7741 gatcaactgg cggaagaaaa agttcgcgac gcgctaaaac cgccatctat gtataaagtg
     7801 atattagtca atgatgatta cactccgatg gagtttgtta ttgacgtgtt acaaaaattc
     7861 ttttcttatg atgtagaacg tgcaacgcaa ttgatgctcg ctgttcacta ccaggggaag
     7921 gccatttgcg gagtctttac cgccgaggtt gcagaaacca aagtggcgat ggtgaacaag
     7981 tacgcgaggg agaatgagca tccattgctg tgtacgctag aaaaagcctg aatgcaggca
     8041 taaaaattgg gggaggtgcc tatgctcaat caagaactgg aactcagttt aaatatggct
     8101 ttcgccagag cgcgcgagca ccgtcatgag tttatgaccg tcgagcactt gttactggcg
     8161 ctgctcagta acccatctgc ccgggaggcg ctggaagcgt gttctgtgga tttggttgcg
     8221 ctccgtcagg aactggaagc ctttattgaa caaaccacac ccgttctgcc tgccagtgaa
     8281 gaggagcgcg acacacagcc gacgctgagt tttcagcgtg tactgcaacg tgcggtcttc
     8341 catgtccagt cctccggtcg caatgaggtt accggtgcaa acgttctggt cgctatcttt
     8401 agcgaacagg agtcgcaggc ggcatatctg ttgcgtaaac atgaagtcag ccgtctcgat
     8461 gtggtgaact ttatctctca tggcacgcgt aaagacgagc cgacacagtc ttctgatcct
     8521 ggcagccagc caaacagcga agaacaagct ggtggggagg aacgtatgga gaatttcacg
     8581 acgaacctga atcagcttgc gcgcgtgggc ggaatcgacc cactgattgg tcgtgagaag
     8641 gagctggagc gtgctattca ggttctctgc cgtcgccgta aaaacaaccc gctgctagtg
     8701 ggggaatctg gtgtcggtaa aaccgcgatt gcagaaggtc ttgcctggcg aattgttcag
     8761 ggcgatgtgc cggaagtgat ggctgactgt acgatttact ctctcgatat cggttctctg
     8821 ttagcgggca caaaatatcg cggcgacttt gaaaaacgtt ttaaagcgtt gctcaagcag
     8881 ctggagcagg acactaacag catcctgttt attgatgaga tccacaccat tatcggtgcg
     8941 ggtgcagcgt ctggtggtca ggttgatgcg gctaacctaa tcaaaccgtt gctctccagc
     9001 ggtaaaattc gcgtaattgg ttcgacaacc tatcaggagt tcagcaacat tttcgagaaa
     9061 gaccgtgctc tggcgcgtcg cttccagaaa attgatatta ctgaaccgtc gatcgaagaa
     9121 actgttcaaa tcatcaatgg cctgaaaccg aagtatgaag cacaccacga cgtgcgttat
     9181 accgcaaaag cggtgcgtgc ggcggtagag ctggcggtga aatacattaa cgatcgtcat
     9241 ctgccggata aagccattga tgttatcgac gaagcgggcg ctcgcgcacg tctgatgccg
     9301 gtaagcaaac gcaagaaaac cgttaatgtg gcggatattg agtccgtggt ggcccgtatt
     9361 gcacgcattc cagagaagag tgtttctcag agtgaccgcg ataccctgaa aaacctcggc
     9421 gatcgcctga aaatgctggt cttcggtcag gataaagcca ttgaggcgct gactgaagcc
     9481 attaagatgg cgcgtgcagg tttaggtcac gaacataaac cggtcggttc gttcctgttt
     9541 gccggcccta ccggggtcgg gaaaacagag gtgacggtac agctttcgaa agctttgggc
     9601 attgagcttc tgcgctttga tatgtccgag tatatggaac gccataccgt cagccgtctt
     9661 attggtgcgc ctccgggata cgttggtttt gatcagggcg gtttgctgac tgatgcggtc
     9721 atcaagcatc cacatgcggt gctgctgctg gacgaaatcg agaaagcgca cccggacgtg
     9781 ttcaatattc tgttgcaggt gatggacaac ggtacgctga ccgataacaa cggacgcaaa
     9841 gcggacttcc gtaacgtggt actggtgatg accactaacg ccggggtacg ggaaactgag
     9901 cgcaaatcca ttggtcttat ccaccaggat aacagcaccg atgcgatgga agagatcaag
     9961 aagatcttta caccggaatt ccgtaaccgt ctcgacaaca ttatctggtt cgatcatctg
    10021 tcaactgacg tgatccatca ggtggtggat aaattcatcg tcgagttgca ggttcagctg
    10081 gatcagaaag gtgtttctct ggaagtgagc caggaagcgc gtaactggct ggccgagaaa
    10141 ggttacgacc gggcaatggg cgcacgtccg atggcgcgtg tcatccagga caacctgaaa
    10201 aaaccgctcg ccaacgaact gctgtttggt tcgctggtgg acggcggtca ggtcaccgtc
    10261 gcgctggata aagagaaaaa tgagctgact tacggattcc agagtgcaca aaagcacaag
    10321 gcggaagcag cgcattaatc tgattgtcag gtaggttggt gaagtccgta atctcgaaag
    10381 aggttacgga ctttttattt atggggtgga acggtgaaaa ccctattttt ggaggtgaag
    10441 gtaatttgtt gataattagt gctgctggaa aaggtaagga taaaaaaggg tgacagcagg
    10501 aaaatgagat ggttttgctt tattaacaac gggctaaacg tgtagtattt gagttcactg
    10561 ccgtacaggc agcttagaaa ttcactggta acatactcca cccgcccacc atgttcactg
    10621 ccgtacaggc agataaaatg cgaaaaaaaa gcccgtactt tcgtacgagc tcttctttaa
    10681 atatggcggt gaggggggga ttcgaacccc cgatacgttg ccgtatacac actttccagg
    10741 cgtgctcctt cagccactcg gacacctcac caaattgttt tgctgccaaa cctcatgggt
    10801 ggcaacgggg cgttactata gggagttgga gtaaaacggt caagaagaat tttaatgata
    10861 attattgttt gctcatactg taaacaactt gtgcagtata tctacatcga gacaagttat
    10921 ggacttatac ttccaaagta cttcatgcat atcacaaaat aaaaaggccg gttaaaccga
    10981 ccttttactc gttctttctc ttcgcccatc aggcggtaaa acaatcagcg actacggaag
    11041 acaatgcggc ctttgctcag gtcgtacggg gtcagttcaa cagtcacttt gtcgcccgtc
    11101 aggatgcgga tgtagttttt gcgcatttta ccggagatgt gtgcagtaac cacgtgaccg
    11161 ttttctaact ctacgcggaa catggtatta ggcaacgttt caagaacggt accttgcatt
    11221 tcaatattgt cttctttggc catctaatcc tctggggtat cactaccgta atttgaaccg
    11281 gcaagataat gccgaagttc tgtaaataag taaagatttg cgcgctaaat cgcaacaaac
    11341 aggttcggca cattactccg aaaacacacg gctaagccgc accaaaagcg caacgtataa
    11401 gggagcggtg agataaacga tgggcgttac ctgacgcgaa aaattcctta tcggcagcgg
    11461 ggtaatgagc gtaaccaact ctgcgaccgc aattataaca ctctggggag aaatgtgccg
    11521 aaaacattca ttcttgtggt gaaaacaagc atcgtggtac ccagaaatta ttcggcaatc
    11581 gtccgaggcg catttgattg agataattaa ggtaattccg gcggggaatt tcgcaggcac
    11641 caagcgatgc tgtgtgatcg ttaaggacct ggcagtcgat aagcttaccg ccatgaccga
    11701 taaattcctc acagaatacc agaagcgccg ttttagacgc attttccatc cggctgaaca
    11761 tggactcgcc acaaaatagc gttccctggg ccacgccgta cataccgccg acaagctcat
    11821 cttcacgcca gacttcaatg gagtgggcat gaccgagttc gtgaaggcga tggtaggctt
    11881 cgaccacgcc acgcgtgatc caggttcctt cttcgcgatc gctggcacag ccttcaatga
    11941 cctgaccaaa agcgtaattc atcgtgacac gatagggcga gcgtttatga aatcgcttca
    12001 tactacggct gatatgcagt gattctggcc atagcaccgc gcggggatcg ggcgaccacc
    12061 agaggatggg gtcgcctgga gaaaaccacg gaaaaatacc acgctggtaa gccattaaca
    12121 ggcgcgcagg gctaagatcg cccccaagtg ccagcaggcc gttaggctca cgtaatgcgc
    12181 cttccgggga agggaaggct attgaatggc gagaaagctg aaccaggcgc atgaccgcaa
    12241 aactccacgc aagtcggatc gttcaataat agcttacaaa ccctgcttga actggtaata
    12301 acgcccctgt ctggcaagca gttctgcgtg agtaccttgc tcaataattt gcccgttgtc
    12361 catcactatt atttgttgga aacgagagag tccgcgaagt cgatgggtga ccattaacac
    12421 cgttttctca cgcatcattt ctgcaagcaa ttcaaggatc tggctttcgg ttgtggcatc
    12481 taagccttcg gtaggttcat ccagcaacac cagtggcgca tcatgtaaca gcgcacgggc
    12541 gatagccaga cggcgcagtt caccaccgga gagctggcgt ccgccttcac ctaaccaact
    12601 gttgagacct gcatcctcga gctgcttctc cagaccaacg cgacgtaaga tcaccgccag
    12661 agcctcatca ctactgccag gcgaggcgag taacagatta tcacgcagcg tggcgctaaa
    12721 cagatgcact cgctgaggaa caacgctgat ggtctgtcgt agagccgctt cattcaggct
    12781 ggctatgggg ctatcgttaa gcaaaatctc gccctgttgc gggtcccatg cgcgggtcag
    12841 cagttgtaac agtgttgatt tgccgcatcc ggttcgcccg agaatcgcta tatgttcccc
    12901 ggcgtttacc tgaagagaaa tccctttaag tgcctgttgg gattgctccg gataagtgaa
    12961 ctgaacatcc cgtaatgtca gcgaaacgcg atcggcaaca cgagtttggg tatcagggaa
    13021 ggtgacctcc ggtttttgat ccgttaagtc agagatacgt acggcagagg caatgacttg
    13081 ccccagatgc tgaaatgcac ccgttactgg tgccagtgct tcaaacgcgg ctaacgcgca
    13141 aaagacaaac agggcaatta acgcgccggg ttgagcattg ccgccaacgc cgccagacgc
    13201 catccacagc atcaggatca ccgctaacgc gccaatgagc agcattatcg cttgcgacaa
    13261 tgcggtcagt tcagattgac ggcgttgcgc ttccagccat tgaatttctg tattctctag
    13321 ttgcgtgcga taacgatcgc tggcaccaaa aatggtcagc tcagcttgcc cttgcagcca
    13381 ggccgtcagt tgttggcgat actgtccgcg aagatgagtc agattttgcc cggtgctttt
    13441 tcccgcacga taaaacagcg gtggcatcag gaaaagcgtc agtaacataa tgccgcccag
    13501 cgtaaaggcg aggggggaat caaggaaact taacccgatt gtcaccacca taatcaccac
    13561 aaaagcgccc accagcggcg agataacgcg caggtaaaga tgatcgagcg tatcaacatc
    13621 cgccaccacg cggttgagca attcgccctg acgatagcgc gccagtccgg caggggagag
    13681 gggcaacaat ttgctgaagg tgtaaatgcg cagatgctgc aacacgcgga aagtcgcgtc
    13741 gtgacttacc agacgttcaa aataacgccc ggcagtacgg gtgattgctg caccacgcac
    13801 gcccgcagcg ggtagcatat agttgaagct gtacagtccg gcaacccccg caaccgctga
    13861 ggccgagagg aaccagccgg aaagtgtcaa cagaccgata ctggcgagca gcgtcacaat
    13921 tgccagcaca ataccaagac ttaacatcca tttatgacgt ttatacagtg ccagataggg
    13981 tagcaaagcg cgcatttaaa tctcctcctg acgatgggcc agtaatgtgg cgaatgggcc
    14041 accagccaca cttaattccg cgtaacgtcc ttgctcaata atccggccat cctgcataac
    14101 ccaaatgaca tcccagtcag caagatcttc taactggtgg gtgaccatta acgttgtctg
    14161 gcgcagagag gcggcattca gcgcctccat tacgcgctgt tcactgtgag catcaaggct
    14221 ggcagcgggt tcatccaaca gtaatagcga acagggattt agtaacgcac gggccaccgc
    14281 cacgcgctgc gcctgcccca cggaaaggcg ggcagcctgg tcgccaacag gcgtatcaac
    14341 gccttgtggc aggagcggta gaaactcgct gacccaggcg ttatccagcg ctgcttgtaa
    14401 ttcttgttcg ctggcatcag gtcgcgccag tagtacgtta tcccgtaatg ttgctgccgg
    14461 taattgtggg ttttgaccaa cccaggagag atgtttacgc catgattctg gtgataaatc
    14521 gcgtaattct atcccgttga ttcgtaatga tccctgatat gagagaaaac cagaaagcgc
    14581 gttcagcagt gagcttttac ctgaaccgct gcgaccaacc aacactgcac gttggcctgc
    14641 tggcaaagta aagttcagcg gtccggccag cgttttacct tccggcgacg tgataaacag
    14701 atcctcggcc tcaatggtca gcggatcggt tgatgctaat tccgtctcac cgcgctgcgg
    14761 atgggcgagc ggggtttcca taaacgtttt cagactgtca gctgcaccaa cagcctgggc
    14821 tttagcatga taaaacgtac cgagatcgcg taatggctgg aaaaactctg gcgcaaggat
    14881 cagggccaga aaacccgcag ccagcgtcac gccagtatcg tagtgaccaa aatccagctc
    14941 gccgagatag gaaaaaccaa agtagaccgc caccagagca attgacagcg aggtaaaaaa
    15001 ttcgagaatg ccggaggata aaaacgccag ccgtagcact tccattgtcc gttggcggaa
    15061 atcttccgaa gcagaacgaa tactttcaat ttcagcttca ccacgaccaa aaatacgcaa
    15121 tgtttccatg ccgcgcaggc gatcgaggaa atgcccactt aagcgagcaa gagcgagaaa
    15181 gttacgtcgg ttagcatcgg cagcccccat cccaaccagc gccataaaca acggaattaa
    15241 cggtgcagtg cccagcagaa tgagcgccgc agcccagtta gaggggaaga tcgccaccac
    15301 aatcagcaac ggcaccgaca ctgccagcgc catttgcggc aggtagcgtg catagtaatc
    15361 atgcatatcg tcaatttgct cgagtaccag cgtcgcccag ctccccgcag gtttaccctg
    15421 aatccacgct ggccctgctt gttgcagacg gtcgagaacc tgacggcgga tggcaaagcg
    15481 gatatgctgc ccggcgtgat aacccacccg ttcgcgtaac cagaccaccc atgcgcgcag
    15541 tacaaaggtc agaaccagta acgtaaaggg aagcagcagg gcttcacggg gaatattctc
    15601 cataatcata tgttgcagaa ttcgcgccat gaaccatgcc tgggcaatga tcaatatgcc
    15661 gctcacaaag cccagcagac gagaaatatt cagccaacgt tgggagatga cgctttgctg
    15721 ttttaaccag cgggttaact ctttttgacg agatttattc attgcacgct tagcaggtga
    15781 gttatcagaa ttatttgcag agcaatgtta caacggggaa aaaataaagg cgacccatag
    15841 tcgcatggtg tcgccttctt tacttttgtt actgatttgt aaaattattt tgcgtcagct
    15901 aaaccatcga ggtagcgttc cgcatcaagt gctgccatgc agcctgtacc agcagatgta
    15961 atggcctggc gataaatgtg atccatcacg tcgcctgcgg caaagacgcc aggaatgctg
    16021 gtctgggtgg cattaccatg aatacccgac tgcactttga tgtagccgtt ttccagttcc
    16081 agctgccctt cgaaaatggc agtattcggg ctgtgaccga tagcaacaaa cagaccggca
    16141 acgtcgagtg actcgatgtt atcgctgttt tgcgtatcgc gcagacgaac gccagtgaca
    16201 cccatttgat cgccggtcac ttcttccagc gtacggttgg tgtgcagaat gatgttgccg
    16261 ttctccactt tatccatcag acgcttaatg aggatttttt ccgcgcggaa accgtcacgg
    16321 cggtgaatca gatgcacttc cgaagcgatg ttagacagat acagcgcctc ttcaaccgcg
    16381 gtattgccgc cgccgatgac cgcaactttc tggttgcgat agaagaaacc gtcgcaggtt
    16441 gcacaagcag aaactccacg gcctttaaag gcttcttcag agggcaggcc gagatagcgt
    16501 gcagaagctc cggtggcaat aatcagcgcg tcgcaagtgt attcgccgtt atcgccattc
    16561 agacggaacg gacggttttg cagatccacc ttgttgatat gatcaaaaat gatctcagtt
    16621 tcaaacttgg tggcatgttc gtgcatgcgc tccattaata acggaccggt cagatcgttt
    16681 ggatcgccag gccagttttc cacttccgtg gtggtggtca gttggccgcc tttttccatg
    16741 ccggtaatca gcacaggttg caggttggcg cgcgccgcat agacagcagc ggtgtatccc
    16801 gccgggcctg aacccaggat aagcagttta ctgtgtttgg tcgtgcccat gagatcccca
    16861 tagttgttgg cagacaatgg gcaggattgt agggaattta cagacgtaaa aaaagagtat
    16921 gacgattttg ttaacaattt gtgcaatcgg cagcatcgat aagcaggtca aattctcccg
    16981 tcattatcac ctctgctact taaatttccc gctttataag ccgattaaat gatgaataaa
    17041 cgcccctgtt aatgaatatc tggcatgttg tactaaaaat cgatgttttg ctttgacaat
    17101 cccctggtgt tttgcgaaaa cattcgagga agaaaaaaaa cagtattctt atatgcgcat
    17161 aaccatgcat gtaaatacca tgtttaccgt gctagtgaaa tctacgtatg gcgtggacag
    17221 acgccattcg tgatgtcgat agctgccaca aggcaacggt cttctcaccg tagacccagg
    17281 cattgcgcgc cgtgaatctt catgatttcg gtctatcgtg acgggtagcg actctgaaca
    17341 gtgatgtttc agggtcagac aggagtaggg aaggaataca gagagacaat aataatggta
    17401 gatagcaaga agcgccctgg caaagatctc gaccgtatcg atcgtaacat tcttaatgag
    17461 ttgcaaaagg atgggcgtat ttctaacgtc gagctttcta aacgtgtggg actttcccca
    17521 acgccgtgcc ttgagcgtgt gcgtcggctg gaaagacaag ggtttattca gggctatacg
    17581 gcgctgctta acccccatta tctggatgca tcacttctgg tattcgttga gattactctg
    17641 aatcgtggcg caccggatgt gtttgaacaa ttcaataccg ctgtacaaaa acttgaagaa
    17701 attcaggagt gtcatttagt atctggtgat ttcgactacc tgttgaaaac acgcgtgccg
    17761 gatatgtcag cctaccgtaa gttgctgggg gaaaccctgc tgcgtctgcc tggcgtcaat
    17821 gacacacgga catacgttgt tatggaagaa gtcaagcaga gtaatcgtct ggttattaag
    17881 acgcgctaac acggaacagg tgcaaaatcg gcgtattttg attacactcc tgttaatcca
    17941 tacagcaaca gtactggggt aacctggtac tgttgtccgt tttagcatcg ggcaggaaaa
    18001 gcctgtaacc tggagagcct ttcttgagcc aggaatacac tgaagacaaa gaagtcacat
    18061 tgacaaagtt aagtagcggc cgccgccttc tggaggcgtt gctgatcctt attgtcctgt
    18121 ttgccgtctg gttgatggct gccttactaa gctttaaccc ttcggacccc agctggtcgc
    18181 aaacggcctg gcatgaacct atccataatt taggtgggat gcccggtgcg tggttggcag
    18241 atacgctgtt ctttattttt ggcgtgatgg cttacaccat tcccgtcatt attgtcggcg
    18301 gttgttggtt tgcctggcgt catcagtcca gcgacgaaaa cattgattat tttgccgttt
    18361 cgctacgcat cattggcgtt ttggcgctca tccttacctc ctgtggtctg gcggcaatca
    18421 acgctgacga tatctggtat tttgcctccg gtggcgtcat tggcagctta ctaagcacta
    18481 cgctacaacc actgctacac agtagcgggg gaactattgc gctgctctgc gtttgggcag
    18541 cgggcctgac gttgttcacc ggttggtcat gggtgaccat tgctgaaaaa ctcggcggct
    18601 ggattttaaa cattctcacc ttcgccagta atcgtacccg tcgcgatgat acctgggtcg
    18661 atgaagatga gtatgaagac gacgaagagt atgaagatga aaaccacggc aaacagcatg
    18721 aatcacgccg tgcccgtatt cttcgcggcg cgctagcgcg tcgtaaacgg ttggcggaaa
    18781 aattcattaa tccgatgggg cggcaaacag acgctgcgtt gttctccggt aagcggatgg
    18841 atgatgacga agagattacc tacactgcac gcggtgtggc tgccgacccg gacgacgtcc
    18901 tattttcggg caatcgtgca acgcagccag aatatgacga atacgatcca ttattaaacg
    18961 gtgcgccaat taccgaacct gtcgctgtag cagctgctgc taccacggcg acacaaagct
    19021 gggctgcgcc ggttgaacct gtgactcaga cgccgcctgt tgcctctgtt gatgttccac
    19081 cttcgcaacc tacagtagcc tggcagcctg taccgggtcc acaaacggga gagccggtta
    19141 ttgctcctgc accggaaggt tacccacagc agtcacaata tgcgcagcct gcagtgcaat
    19201 ataatgagcc gctgcaacaa ccagtacagc cgcagcagcc gtattatgca cctgcagctg
    19261 aacaacctgc gcaacagccg tattatgccc ctgcgccaga acaaccggtg gcaggtaacg
    19321 cctggcaagc cgaagagcag caatccactt ttgctccaca gtctacatac cagactgagc
    19381 aaacttatca gcagccagcc gctcaggagc cgttgtacca acagccgcaa cccgttgaac
    19441 agcagcctgt tgtggagcct gaacccgttg tagaagagac aaaacccgcg cgtccgccgc
    19501 tttactactt tgaagaagtt gaagagaagc gagcccgtga acgtgaacaa cttgcggcct
    19561 ggtatcaacc gattccagaa ccggttaaag aaccagaacc gatcaaatct tcgctgaaag
    19621 caccttctgt tgcagcagta cctccagtag aagccgctgc cgctgtttct ccgctggcat
    19681 ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc aaccgttgcc gcgccagtct
    19741 tcagtctggc aaatagcggt ggaccgcgtc cgcaggtcaa agaggggatt ggcccgcagt
    19801 tgccacgacc gaaacgtatc cgcgtgccaa ctcgtcgtga attggcgtct tacggtatta
    19861 agctgccctc acagcgtgcg gcggaagaaa aagcccgtga agcccagcgc aatcagtacg
    19921 attctggcga tcagtacaac gatgatgaaa tcgatgcgat gcagcaggat gaactggcac
    19981 gtcagttcgc ccagacacag cagcaacgct atggcgaaca gtatcaacat gatgtgcccg
    20041 taaatgcaga agatgcagat gctgcggcag aggctgaact ggctcgtcag tttgcgcaaa
    20101 ctcaacaaca acgttattcc ggcgaacaac cggctggggc gaatccgttc tcgctggatg
    20161 attttgaatt ttcgccaatg aaagcgttgc tggatgatgg tccacacgaa ccgctgttta
    20221 cgccaattgt tgaacctgta cagcagccgc aacaaccggt tgcaccgcag cagcagtatc
    20281 agcagccaca acagccggtt gcgccacagc cgcagtatca gcagccacaa cagcaggttg
    20341 cgccgcagcc acaatatcag cagccgcaac aaccggttgc gccacagcag caatatcagc
    20401 agccgcaaca accagttgcg ccgcagccgc agtatcaaca gccgcaacaa ccggttgcac
    20461 cgcagccgca gtatcagcag ccacaacagc cagttgcgcc acagccgcag gatactctgc
    20521 ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt gcataaaccg acgacgccgc
    20581 tgccttctct ggatttgctg acaccgccgc cgagcgaagt ggagccggta gatacctttg
    20641 cgcttgaaca aatggctcgc ctggtggaag cgcgtctggc tgatttccgt attaaagccg
    20701 atgtcgtcaa ttactctccg gggccggtta tcactcgctt tgaacttaac ctggcaccgg
    20761 gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct tgcccgttca ctttcgacgg
    20821 tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtaggtctg gagttaccga
    20881 ataaaaaacg acaaaccgtt tatctgcgcg aagtattgga taacgccaaa ttccgcgata
    20941 atccatcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg gtggttgccg
    21001 atctggcgaa aatgccgcac ttgttagttg cggggactac cggttccggt aaatctgtcg
    21061 gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
    21121 tcatgatcga cccgaaaatg ctggagcttt cggtgtatga aggcattccg cacctgttaa
    21181 cggaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
    21241 tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
    21301 aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac tggaagccgg
    21361 gtgacagtat ggatgcccag catccggtgc tgaaaaaaga accatacatt gtggtgttgg
    21421 tggacgaatt tgccgacctg atgatgacag taggtaaaaa agtggaagag ctgatagcac
    21481 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaact cagcgtccat
    21541 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
    21601 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg
    21661 gtatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
    21721 cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
    21781 cacagtatgt tgatggcatc acctccgaca gcgaaagcga aggtggtgcg ggtggtttcg
    21841 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
    21901 gcaaagcgtc aatttctggc gtacaacgtc agttccgcat tggttataac cgtgcagcgc
    21961 gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac aacggtaatc
    22021 gtgaagtgct gtccccaccg ccgtttgact aattaatgca ttgccggata aggcgcggta
    22081 gcgtcgcatc cggcactcta tcaactgaaa attcagtatt ttcttctttc ctcaagctga
    22141 ttattagcct ggaatagaga gtagagggaa ctcccgatcg ggagtgacgt aatttgagga
    22201 ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt agcaagcagc
    22261 gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag cagcttccac
    22321 gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga aggtcagggc
    22381 gatctgtggg tgaaacgtcc aaacttattc aactggcata tgacacaacc tgatgaaagc
    22441 attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga gcaagctacg
    22501 gcaacctggc tgaaagatgc caccggtaat acgccgttta tgctgattgc ccgcaaccag
    22561 tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt cctgacgccg
    22621 aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga tggcacaatc
    22681 catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcaact gaaatcccag
    22741 caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg cgtcacggta
    22801 gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt tttcggataa
    22861 tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt atatcggtca
    22921 gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct atcgaagccg ggcatttaca
    22981 ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg aagtgattgc
    23041 ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggtg tgaaagagat
    23101 tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca ggtcgccgca ctattctttt
    23161 tgttgacgaa gttcaccgtt tcaacaaaag ccagcaggat gcatttctgc cacatattga
    23221 agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc
    23281 ggcactgctt tcccgtgccc gtgtttatct gttgaaatcc ctgagtacag aggatattga
    23341 gcaagtacta actcaggcga tggaagacaa aacccgtggc tatggtggtc aggatattgt
    23401 tctgccagat gaaacacgac gtgccattgc agaactggtg aatggcgacg cgcgccgggc
    23461 gttaaatacg ctggaaatga tggcggatat ggccgaagtc gatgatagcg gtaagcgggt
    23521 cctgaagcct gaattactga ccgaaatcgc cggtgaacgt agcgcccgct ttgataacaa
    23581 aggcgatcgc ttttacgatc tgatttccgc actgcataag tcggtacgtg gtagcgcacc
    23641 cgatgcggcg ctgtactggt atgcgcgaat tattaccgct ggtggcgatc cgttatatgt
    23701 cgcgcgtcgc tgtctggcga ttgcgtctga agacgtcggt aatgccgatc cacgggcgat
    23761 gcaggtggca attgcggcct gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg
    23821 cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg ctgtctacac
    23881 tgcgtttaaa gccgcgctgg ccgatgctcg cgaacgcccg gattatgacg tgccggttca
    23941 tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg aatatcgtta
    24001 cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc cggaaatagc
    24061 acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg gcgaaaagct
    24121 cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt aatgttatcg
    24181 ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat ctcccattta
    24241 attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg agccagacgc
    24301 agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc tgggcgctct
    24361 tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg agcgtaactc
    24421 ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc ctttacgtct
    24481 ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg atgctttaca
    24541 ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg cctgcagatg aagtgccggt
    24601 aggtaaagac gaaaatgaca acgttgaagt cagccgctgg ggtaccccgc gtgagtttga
    24661 ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac tctggcctcg actttgcagc
    24721 tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa gggcagattg ctcgcatgca
    24781 ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct acagtgagaa
    24841 ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc tgccgaaatt
    24901 tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca gtaactatgc
    24961 gctgatccca actgcagaag ttccgctgac taacctggtg cgcggtgaaa tcatcgatga
    25021 agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg aagccggttc
    25081 atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag ttgaaatggt
    25141 gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg gtcatgcaga
    25201 aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgca ctggcgacat
    25261 gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac agaacaccta
    25321 ccgtgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta tgcaggcacg
    25381 ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg gttctggtct
    25441 ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat cagcaagctg atggtcgtat
    25501 tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg gctaataccc
    25561 aatttttctg aatctaaaaa gcgcctacgg gcgctttttt tgtctccctt tgataccgaa
    25621 caataattac tcctcactta cacgtaatac tactttcgag tgaaaatcta cctatctctt
    25681 tgattttcaa attattcgat gtatacaagc ctatatagcg atctgctata gaaataatta
    25741 cacaatacgg tttgttactg gaatcaatcg tgagcaagct tgagtgagcc attatgaaaa
    25801 cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg gtaaaaacga
    25861 cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt agtcggattg
    25921 cgcacgctgt cgatagcgcc attccaacaa aatcaggcga aaaggttatc tggagcgcct
    25981 gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca
    26041 aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac caggttcgcg
    26101 cctgcctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc
    26161 cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc tgggaagaag
    26221 cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc aacgaatcca
    26281 tctatctgaa ctatggcacc ggtacgctgg gtggcaccat gacccgctcc tggccgccgg
    26341 gaaataccct ggtcgcgcgg ctgatgaact gctgcggcgg ctatctgaac cattacggcg
    26401 actactcctc cgcgcaaatt gcggaaggtc tgaactatac ctacggcggc tgggcagatg
    26461 gcaacagccc gtcggatatc gaaaacagta agctggtagt gctgtttggt aataaccctg
    26521 gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggca cgccagaaat
    26581 ctaatgcccg catgatcatc atcgatccgc gctataccga caccggtgcc gggcgcgaag
    26641 atgagtggat ccctattcgt ccgggaacag atgccgcact ggttaacggt ctggcgtacg
    26701 tcatgatcac tgaaaacctg gtggatcagg cattcctcga taaatattgc gttggctacg
    26761 atgagaaaac cctgccagcc agtgcgccga aaaatggcca ctataaagct tatattctgg
    26821 gtgaagggcc agatggcgtg gctaaaacac cggaatgggc ctcgcaaatc accggtgttc
    26881 cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg gcgtttatca
    26941 gccagggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt gctatctcga
    27001 tgctggcgat tctgaccggt aacgttggta ttaatggagg gaacagcggc gcgcgtgaag
    27061 gttcatacag tttaccgttt gtccgtatgc cgaccttgga aaacccgatc cagaccagca
    27121 tttcgatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgacg gcgctacgtg
    27181 atggtgtgcg cgggaaagat aagctggatg tgccgatcaa aatgatctgg aactatgccg
    27241 gtaactgcct gattaaccag cattctgaaa tcaaccgtac ccatgaaatc cttcaggatg
    27301 ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatcg gcgaaatatg
    27361 ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgca ctggatgcat
    27421 cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg cgctttgaat
    27481 gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt gagcaacagt
    27541 ttactgaagg ccgtacccag gaagagtgga tgcggcatct gtatgcccag tcgcgggaag
    27601 cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg
    27661 acccacaagg gcatcacgtt gcttataaag ccttccgtga agatccgcag gcaaatccac
    27721 tgaccacgcc atcgggtaaa attgagattt attcgcaggc gctggctgac attgccgcta
    27781 cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctacacg ccgggctttg
    27841 aaagttatca ggatccgctg aacaaacagt atccgctgca gcttacaggt ttccactata
    27901 aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcggct tgccgtcagg
    27961 aaatgtggat caacccgctt gatgcccaaa aacgcggtat ccacaacggc gataaagtca
    28021 ggatctttaa cgatcgtggt gaggttcata ttgaggcgaa agtgacgcca cgaatgatgc
    28081 cgggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatgcaaaa cgtgtcgata
    28141 agggtggttg tattaacgta ctgaccactc aacgtccgtc tcctctcgct aaggggaatc
    28201 cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat gacaacccag
    28261 tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga gctggcttgt
    28321 aaagactaca aagatttgat gccagaagtc agcttccgcc gcatttatga atatgctggt
    28381 ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta ctatctgtcg
    28441 atttcgtgca accactgcga agatccggct tgtactaaag tctgcccgag cggtgcgatg
    28501 cataaacgtg aagatggttt tgttgtggtc gatgaagatg tgtgcattgg ctgccgctac
    28561 tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg ccatatgacc
    28621 aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct
    28681 tgtccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa acatggcgat
    28741 ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat tgtgatcaaa
    28801 cccaatgcca atagccgccc gaccggggat accactggct atctggcaaa cccgaaggag
    28861 gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg tcttcgggca
    28921 atgtgtggcg ggtggtttta tcgttctggc tttggcgctg cttaaaggcg acctgcgagc
    28981 agaagcccag cagcgtgtta tcgcctgcat gtttggctta tgggtgctga tgggcattgg
    29041 ctttatcgcc tctatgctcc atcttggttc accgatgcgc gcctttaact cgctcaaccg
    29101 ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt ttgctgtagg
    29161 cggcatcggc tggttgctgg caatgctgaa aaagctgtca cctgcattgc gtacgctatg
    29221 gctggtggtg acgatggttc ttggcgtcgt ctttgtctgg atgatggtgc gtgtgtataa
    29281 cagcattgat accgttccga cctggtacag catctggacg ccgatgggct tcttcctgac
    29341 gatgttcatg ggcggcccgt tactgggtta cctgttgctg agtctggctg gcgtcgatgg
    29401 ctgggcgatg cgtctgctgc cagccatttc tgtactggca ctggtggtga gtggcgtgat
    29461 gtcagtgatg cagggcacag agctggcaac tattcatagt tctgtgcagc aggccgcagc
    29521 gctggtgccg gactatggtg cgctgatgtc ctggcgaatc gtgcttttgg ccgttgccct
    29581 gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc tactatccgt
    29641 ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct atggtttgca
    29701 tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt cggccccggt
    29761 tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac gataatcagg
    29821 aatgtggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt cacggtgcag
    29881 ctcgcaagcc acgccgaacc atgtcatcaa ttgcgcacct gcttgcgaca tgcgatccca
    29941 tgccgaatgc cgggtaattt cattaaaggt ccctgacgcg tcagtcacaa caaagacatc
    30001 aaacccttct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg ttaccacacc
    30061 ggcaataatt aactgttttt tacctgtcgc tttgactgct tttacaaaat cttcgttatc
    30121 ccaggcgtta atattgccag ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag
    30181 ctctggaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa tagttggtaa
    30241 gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact tatcgggttc
    30301 gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa cggcagcatc
    30361 atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag agtcagtggt
    30421 acgtctgaaa tcggtcagac gcgatgcatt gctctgaaag catagacggg aaatatgagt
    30481 ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat
    30541 aaaaacaatc agatgagatt aatcatctct tcagcattag cgttatgaat gagtaatata
    30601 aatcgataat accgccgctg cgatatgcgc gtattttcac ccattgacaa tgttttttgc
    30661 ggtggcatga tgcgcatgaa atttgaactt cctcacggtt ttaattcatg tccacgtata
    30721 cccggcctgt catgcttttg ctgtctggcc tgcttttgtt gactctggcg atcgcggtgt
    30781 taaatacact cgtgccgctt tggctcgccc aggaacacat gtccacatgg caggtaggcg
    30841 ttgtcagctc atcctatttt accggcaacc ttgtcggtac attgctgaca gggtatgtca
    30901 ttaaacgcat tggctttaac cgcagctatt atctggcctc cttcattttt gccgctggct
    30961 gtgcaggcct tggcctgatg attggtttct ggagctggtt ggcgtggcgt tttgtcgcgg
    31021 gcgtcggctg tgccatgatt tgggtagtcg ttgagagcgc gctgatgtgc agtgggacgt
    31081 cacgtaaccg tgggcgtttg cttgctgcgt atatgatggt ttattacgtg ggaacatttt
    31141 taggccagtt actggtcagc aaagtctcaa ccgagctgat gagcgtattg ccgtgggtta
    31201 caggtttgac gttggcaggg atcttaccgc tgttgtttac gcgtgtcctg aatcagcagg
    31261 ctgaaaacca tgattcgacg tcaattacgt caatgctaaa actccgtcag gcgcggcttg
    31321 gcgtgaatgg ctgcattatc tcaggaatcg tgctgggatc tctatatggc ctgatgccgc
    31381 tgtacctcaa tcacaaaggg gtgagcaatg ccagcattgg tttctggatg gcggtactgg
    31441 tcagtgcggg tatcctcgga caatggccga ttggacgtct ggcggataag tttggtcgac
    31501 tgctggtgtt gcgtgttcag gtctttgtcg tcattctcgg cagtatcgcg atgcttagcc
    31561 aggcggcgat ggccccagcg ttattcatcc tcggtgccgc tggctttacg ctatatccgg
    31621 tggcgatggc ctgggcttgt gagaaagttg aacatcatca actggtggcg atgaaccagg
    31681 ccttactgtt gagctatacc gtgggaagtc tgcttggccc gtcatttacc gctatgctaa
    31741 tgcagaattt ctccgataat ttattgttta tcatgatcgc cagcgtatcg tttatctatt
    31801 tgctgatgct gcttcgcaac gccggtcata cgccgaaacc cgttgctcac gtgtaaatga
    31861 attcaagcaa agtgtgaact tactgtttca cactctgctt ttttgtttct tctatctgac
    31921 ttgctttttt ccaaatttta ttcgtttaaa aataaaatgt gcagcaggtt ataattttgc
    31981 atttcgctat ttccgcactt cttatttgcc gcgcataatc cctcgtttta cagatgcccc
    32041 tttaattttg gcgaaggatt tgtctatggc tgggaatgtt caggaaaaac agctgcgatg
    32101 gtacaacatt gcgctgatgt cttttatcac tgtctggggt tttggcaacg ttgttaataa
    32161 ctatgccaac caggggctgg tggttgtttt ttcatgggtg tttatctttg cgctctattt
    32221 cacaccttat gcactaattg ttggtcagtt aggctcgacc ttcaaagatg ggaagggcgg
    32281 ggtcagtacc tggattaaac acacgatggg acccggattg gcttacctcg ccgcgtggac
    32341 ctactgggtg gtgcatattc cctatctggc acaaaaaccc caggcaatac tgattgcgct
    32401 cggttgggcg atgaaaggcg acggttcgct aatcaaagaa tattcagtcg tagcgttaca
    32461 ggggttaacg ctggtgctgt ttatcttctt tatgtgggtt gcttcacgcg gtatgaaatc
    32521 gctgaaaatc gtcggttctg tggcagggat tgcgatgttt gttatgtcgc tcctgtatgt
    32581 ggcgatggcg gtaaccgcgc ctgcgattac tgaagtgcat attgcgacca caaacattac
    32641 ctgggaaacg ttcattcctc atatcgactt tacctacatt accactattt caatgctggt
    32701 tttcgcggtt ggcggagctg agaagatttc tccttacgtt aatcaaacgc gcaacccagg
    32761 aaaagaattt ccaaaaggga tgttatgcct ggcggtgatg gttgcggttt gtgccattct
    32821 gggctcactg gcgatgggga tgatgtttga ctcgcgtaat atcccggatg acttaatgac
    32881 taacggtcag tattacgcct ttcagaagct gggcgagtat tacaacatgg gtaatacttt
    32941 aatggtgatt tacgccattg cgaataccct gggacaagta gctgcgctgg tattctcgat
    33001 tgatgccccg cttaaagtgc tattaggcga tgctgacagc aaatatattc cagccagttt
    33061 atgtcgtacc aacgcttctg gtacgcccgt taatggctat tttctgaccc tggtgctggt
    33121 ggcgatcctg attatgctgc cgactctcgg catcggtgat atgaacaatc tctataaatg
    33181 gctgttgaat cttaactcgg tagtgatgcc gctgcgttat ttatgggtat ttgttgcatt
    33241 tattgcagtc gttcgcctgg cgcagaaata tacaccagag tatgtcttta ttcgtaataa
    33301 gcctctggca atgaccgtcg ggatttggtg ttttgccttt actgcctttg cctgtttgac
    33361 ggggatcttc ccgaaaatgg aagccttcac tgcagagtgg accttccagt tggcgctgaa
    33421 tgttgcaacg ccgtttgtgc tggtaggatt aggactgata ttcccgctgc tggcgcgtaa
    33481 agcgaatagt aaataattat tgtggtcggt gacgctgact ctggcagatc tgacccgttt
    33541 ttacataatc gagaaatgcg cgcaagccgc aggacatata ctggcggttt gggtaataca
    33601 tttggaagct gtgctgtaag ggcgttagaa atagagctct gaattattta tgcagcgcgg
    33661 aataaaaaag cggcaactca ataaagttgc cgctttacgg ggaaattaga acattacctt
    33721 atgaccgtac tgctcaagaa tacctttcac gcgttccatg gtttctttct tcggtggttt
    33781 aacaccatcg agtttgtatt cttcacccat cgccacccat ttgtgtttgc ccagttcgtg
    33841 gtaggggaga agttcgattt tctcaacgtt acccatatca cgggtaaatt caccgaggcg
    33901 atgcgctgaa tcgtcatcgt cagaccagcc tgggacaaca acgtagcgga tccacacctt
    33961 cacattttta ttcgccagat atttggcgaa ctccagcgtg cggtggttgg aaactccaac
    34021 cagattttgg tggatctcgt cgttcatctg tttgagatcg agcattacca ggtcggttac
    34081 ttccagcagt tcatcaatca ccggatcgta acgacgaaca aaaccgttgg tgtccagaca
    34141 ggtatgaatg ccttcttttt tgcaggcgcg gaaccagtca cgaacaaact cagcttgcag
    34201 gattgcttca ccgccggatg cggtaacgcc gccgccggaa gcgttcataa agtggcgata
    34261 ggtcaccact tccttcatca aatcttcaac ggtaacttct ttaccgccat gcgtgtccca
    34321 ggtgtcgcgg ttatgacaat acaggcagcg catcaggcag ccctggaaaa aggtgataaa
    34381 gcgaatacct gggccgtcta cggttccaca ggattcaaag gagtgaatgc gaccaataac
    34441 tgacattgcg gtgtttctcc agatgtggcc catctgaggc cgtgttggtg cgcagctcga
    34501 aggctacgtc gagtctgttt tggcagtcac cttaaagtat agatagctga caaaaaaggc
    34561 tcacgtgcta aaaaaggccc cactttcgtg gagcctttat tgtacgcttt ttactgtacg
    34621 atttcagtca aatctaatta catagattga gtgaaggtac gagtaataac gtcctgctgc
    34681 tgttctttag tcagcgagtt gaaacgtact gcgtagccag atacacggat ggtcagctgc
    34741 ggatattttt ccggattttc catcgcgtcg agcagcattt cacggttcat cacgttaacg
    34801 ttcaggtgct gaccaccttc gatggatgct tcgtggtgga agtaaccatc catcagacca
    34861 gccaggttgg tcttacgaac ttcgtcgtct ttacccagtg cgttcggaac gatagagaag
    34921 gtgtaggaga taccatcttt agcgtaagca aacggcagtt tagcaacgga agtcagagac
    34981 gctacagcac ctttctggtc acgaccgtgc atcgggttag cacccggtcc gaacggcgcg
    35041 ccagcacgac gaccgtctgg ggtgttaccc gttttcttac catacacaac gttagaagtg
    35101 atggtcagaa cagactgagt cgggatagcg tcacggtagg tgtgcagttt ctgaattttc
    35161 ttcatgaaac gttctaccag gtcaacagcc aggtcatcta cacgcggatc gttgttacca
    35221 aactgcgggt attcgccttc gatttcgaag tcgatagcca gaccgtcttc gtcacgaatc
    35281 ggtttaactt tcgcatattt gattgcagac agggagtcag cagcaacgga cagaccagcg
    35341 ataccacacg ccatggtgcg gataacgtca cggtcgtgca gcgccatcag agaggcttcg
    35401 tagctgtact tgtcgtgcat gtagtgaatg atgttcagtg cagtgatgta ctgtttagcc
    35461 agccagtcca tgaagtgatc catgcgctcc atcacttcat cgtagttcag gagatcgcct
    35521 ttgatcggtt cagacttcgg accaacctgc attttcagtt tttcgtcaac gccgccgttg
    35581 attgcgtaca gcatggtttt cgccaggttt gcacgcgcac cgaagaactg catttgttta
    35641 ccaacgatca tcgggcttac gcagcaagcg atagcgtagt catcgttgtt gaagtccgga
    35701 cgcatcaggt catcgttctc atactgcaga gaagaggtgt cgatggacac tttagcggcg
    35761 aatttcttga agttcagcgg cagtttttca gaccacagaa tggtcatgtt cggttccgga
    35821 gacggaccca tggtgtacag ggtgttcagg aaacggaagc tgtttttggt aaccagggta
    35881 cgaccgtcga ggcccatacc accgatagat tcggttgccc agatcgggtc gccagagaac
    35941 agttcatcgt actccggagt acgcaggaag cgaaccatac gcagtttcat gaccaggtgg
    36001 tcaaccattt cctgcgcttc ttgttcggtg atcttgccag ctttcaggtc acgttcgatg
    36061 tacacatcca ggaaggtgga ggtacgaccg aaggacattg cagcaccgtt ctgagactta
    36121 acagcagcca ggtagccgaa gtaagtccac tggatagctt cctgagcgtt ggtagccgga
    36181 ccagagatgt cgtagccgta tttcgcagcc atttctttca tctgacccag agcgcggtgc
    36241 tgttcagcga tttcttcgcg cagacggata gtctgttcca ggtttacgcc gttttccaga
    36301 tcagcctgca gagaggtgaa ctgagcgtat ttgtctttca tcaggtagtc gataccgtac
    36361 agcgcaacgc gacggtagtc accgatgata cggccacggc cataagcatc tggcagaccg
    36421 gtcagaacac cggatttacg gcaacgcagg atgtccggag tgtaaacgtc gaacacgccc
    36481 tggttgtgag ttttacggta ttcagtgaag atttttttga tcatcgggtc cagttcgcgg
    36541 ttgtacgctt tgcaggaacc ttcgatcatt ttgataccac cgaacgggat aagagcacgt
    36601 ttcagcggag cttcagtctg cagaccaaca actttttcca acgctttgtt gatgtagcca
    36661 gcgtcgtgag aggtgatggt ggaagcaaca gcggtgtcga agtcaactgg cgcgtgagtg
    36721 cggttttcca gtttaacgcc ttccattact ttgtcccaca gggtggtggt cgcttcagta
    36781 gcgccagcca ggaaggactc gtcaccctcg tacggagtgt agtttttctg aatgaagtca
    36841 cggacgttta cttcattctg ccagtcacct ttggtaaaac cttcccaggc tgtggctaac
    36901 ttttcattaa gctcggacat gtaacaccta ccttcttaag tggatttttt atttactgcg
    36961 tacttcgaca accattaatg gtgatcgttt tcacgcaggt aaatgaccca gtatgtcaac
    37021 ccaaccaaca aaccaccgcc gataatatta ccgatcgtaa ccggaatcag gttatcagtg
    37081 atgaagttca tcacggtcag gtgagaaaaa ttttccggtg cagaaccgac agcggtccag
    37141 aattccggag atgcgaagtc gcggattaca atacccatcg ggatcataaa catgtttgcg
    37201 atactgtgct caaaaccgct ggcaacaaac atcgcgaccg gcagcaccat gataaacgct
    37261 ttgtccatca ggctgcggcc agaataactc atccatactg ccagacatac catcaggttt
    37321 gccaggatac caaggcagac ggcctcaata aaagtatggt gcactttgtg gtcggcggtt
    37381 tgtaggacgt ttagtcccca ttgaccattt gcggtcatat actcgccgga aagccacatt
    37441 aaaagtacaa acagcagtgc gccgaccagg ttgccaaaat agacatttag ccagttttta
    37501 gccaactgac cccaggtgat gcgcccactc gccttagcaa caacaatcaa cactgtggaa
    37561 gtaaagagat cggctccgca gacaacacaa agaatcagtc ccagagagaa gcaaatgccg
    37621 ccaaccagtt ttgccatgcc gaagggcatt gtgcctgtgc cagtggttgc tgtgatatag
    37681 aagacgaatg cgattgagat gaaaacaccg gcggtaatcg ccagatagaa agtcttaagc
    37741 ggatgtttcg ttgctttata gacacccgcc tcttcggcca ctttggccat tgcagcagga
    37801 agtaaaagat caaaagggtt gtcagctttc acactaactc tctctttatt aagtcggcga
    37861 cgagatacta acaaagcatt atagatgaga aattgatata gatcatatct cgcctggctt
    37921 ataggcccgt aactcgcatg gttttta
//
LOCUS       DAZKIF010000030        37874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZKIF010000030 DAZKIF010000000
VERSION     DAZKIF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.030"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..883
                     /gene="ytfI"
                     /locus_tag="ACHOTW_001578"
     CDS             <1..883
                     /gene="ytfI"
                     /locus_tag="ACHOTW_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFP9843707.1"
                     /translation="SSYTKTHSNNFFNNIHHAFKKKDWISNYDSLLTLREFFRCATQI
                     DKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYTMTLRGSGFEYEEIPITINEYNSF
                     MDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQAIGDVSLGYALLDDIVSKL
                     KRGKFELQGPGGGIKQCDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVV
                     LEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSDITKEKICEKYKSTIEAFK
                     KEQLALPVLVRRKNSET"
     gene            complement(946..1500)
                     /gene="ytfJ"
                     /locus_tag="ACHOTW_001579"
     CDS             complement(946..1500)
                     /gene="ytfJ"
                     /locus_tag="ACHOTW_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP9843708.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1825..2031
                     /gene="ytfK"
                     /locus_tag="ACHOTW_001580"
     CDS             1825..2031
                     /gene="ytfK"
                     /locus_tag="ACHOTW_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP9843709.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2127..3470)
                     /gene="paeA"
                     /locus_tag="ACHOTW_001581"
     CDS             complement(2127..3470)
                     /gene="paeA"
                     /locus_tag="ACHOTW_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9843710.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3793..4431)
                     /gene="msrA"
                     /locus_tag="ACHOTW_001582"
     CDS             complement(3793..4431)
                     /gene="msrA"
                     /locus_tag="ACHOTW_001582"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9843711.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4637..6370
                     /gene="tamA"
                     /locus_tag="ACHOTW_001583"
     CDS             4637..6370
                     /gene="tamA"
                     /locus_tag="ACHOTW_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP9843712.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6367..10146
                     /gene="tamB"
                     /locus_tag="ACHOTW_001584"
     CDS             6367..10146
                     /gene="tamB"
                     /locus_tag="ACHOTW_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP9843713.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10149..10490
                     /gene="ytfP"
                     /locus_tag="ACHOTW_001585"
     CDS             10149..10490
                     /gene="ytfP"
                     /locus_tag="ACHOTW_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP9843714.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10702..10953
                     /gene="chpS"
                     /locus_tag="ACHOTW_001586"
     CDS             10702..10953
                     /gene="chpS"
                     /locus_tag="ACHOTW_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFP9843715.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10947..11297
                     /gene="chpB"
                     /locus_tag="ACHOTW_001587"
     CDS             10947..11297
                     /gene="chpB"
                     /locus_tag="ACHOTW_001587"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFP9843716.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(11377..11907)
                     /gene="ppa"
                     /locus_tag="ACHOTW_001588"
     CDS             complement(11377..11907)
                     /gene="ppa"
                     /locus_tag="ACHOTW_001588"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP9843717.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12217..13173
                     /gene="ytfQ"
                     /locus_tag="ACHOTW_001589"
     CDS             12217..13173
                     /gene="ytfQ"
                     /locus_tag="ACHOTW_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP9843718.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13313..14815
                     /gene="ytfR"
                     /locus_tag="ACHOTW_001590"
     CDS             13313..14815
                     /gene="ytfR"
                     /locus_tag="ACHOTW_001590"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP9843719.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQARRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14829..15851
                     /gene="ytfT"
                     /locus_tag="ACHOTW_001591"
     CDS             14829..15851
                     /gene="ytfT"
                     /locus_tag="ACHOTW_001591"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP9843720.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15838..16833
                     /gene="yjfF"
                     /locus_tag="ACHOTW_001592"
     CDS             15838..16833
                     /gene="yjfF"
                     /locus_tag="ACHOTW_001592"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP9843721.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16866..17864)
                     /gene="fbp"
                     /locus_tag="ACHOTW_001593"
     CDS             complement(16866..17864)
                     /gene="fbp"
                     /locus_tag="ACHOTW_001593"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP9843722.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            18040..19413
                     /gene="mpl"
                     /locus_tag="ACHOTW_001594"
     CDS             18040..19413
                     /gene="mpl"
                     /locus_tag="ACHOTW_001594"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP9843723.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19569..20120)
                     /gene="yjgA"
                     /locus_tag="ACHOTW_001595"
     CDS             complement(19569..20120)
                     /gene="yjgA"
                     /locus_tag="ACHOTW_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP9843724.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20214..21566
                     /gene="pmbA"
                     /locus_tag="ACHOTW_001596"
     CDS             20214..21566
                     /gene="pmbA"
                     /locus_tag="ACHOTW_001596"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP9843725.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21750..22136
                     /gene="cybC"
                     /locus_tag="ACHOTW_001597"
     CDS             21750..22136
                     /gene="cybC"
                     /locus_tag="ACHOTW_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9843726.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(22181..22645)
                     /gene="nrdG"
                     /locus_tag="ACHOTW_001598"
     CDS             complement(22181..22645)
                     /gene="nrdG"
                     /locus_tag="ACHOTW_001598"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP9843727.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22835..24973)
                     /gene="nrdD"
                     /locus_tag="ACHOTW_001599"
     CDS             complement(22835..24973)
                     /gene="nrdD"
                     /locus_tag="ACHOTW_001599"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9843728.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25367..27022)
                     /gene="treC"
                     /locus_tag="ACHOTW_001600"
     CDS             complement(25367..27022)
                     /gene="treC"
                     /locus_tag="ACHOTW_001600"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9843729.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(27072..28493)
                     /gene="treB"
                     /locus_tag="ACHOTW_001601"
     CDS             complement(27072..28493)
                     /gene="treB"
                     /locus_tag="ACHOTW_001601"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP9843730.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28612..29559)
                     /gene="treR"
                     /locus_tag="ACHOTW_001602"
     CDS             complement(28612..29559)
                     /gene="treR"
                     /locus_tag="ACHOTW_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP9843731.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTERSEPQQIIIPANLS"
     gene            29744..29797
                     /gene="mgtL"
                     /locus_tag="ACHOTW_001603"
     CDS             29744..29797
                     /gene="mgtL"
                     /locus_tag="ACHOTW_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP9843732.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29938..32634
                     /gene="mgtA"
                     /locus_tag="ACHOTW_001604"
     CDS             29938..32634
                     /gene="mgtA"
                     /locus_tag="ACHOTW_001604"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9843733.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32840..33226)
                     /gene="ridA"
                     /locus_tag="ACHOTW_001605"
     CDS             complement(32840..33226)
                     /gene="ridA"
                     /locus_tag="ACHOTW_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP9843734.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33299..33760)
                     /gene="pyrI"
                     /locus_tag="ACHOTW_001606"
     CDS             complement(33299..33760)
                     /gene="pyrI"
                     /locus_tag="ACHOTW_001606"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP9843735.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33773..34708)
                     /gene="pyrB"
                     /locus_tag="ACHOTW_001607"
     CDS             complement(33773..34708)
                     /gene="pyrB"
                     /locus_tag="ACHOTW_001607"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP9843736.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34712..34846)
                     /gene="pyrL"
                     /locus_tag="ACHOTW_001608"
     CDS             complement(34712..34846)
                     /gene="pyrL"
                     /locus_tag="ACHOTW_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP9843737.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35127..35522)
                     /gene="yjgH"
                     /locus_tag="ACHOTW_001609"
     CDS             complement(35127..35522)
                     /gene="yjgH"
                     /locus_tag="ACHOTW_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9843738.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35653..36366)
                     /gene="bdcA"
                     /locus_tag="ACHOTW_001610"
     CDS             complement(35653..36366)
                     /gene="bdcA"
                     /locus_tag="ACHOTW_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9843739.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36437..37030
                     /gene="bdcR"
                     /locus_tag="ACHOTW_001611"
     CDS             36437..37030
                     /gene="bdcR"
                     /locus_tag="ACHOTW_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9843740.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            37175..37627
                     /gene="tabA"
                     /locus_tag="ACHOTW_001612"
     CDS             37175..37627
                     /gene="tabA"
                     /locus_tag="ACHOTW_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP9843741.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37750..>37874
                     /locus_tag="ACHOTW_001613"
     CDS             37750..>37874
                     /locus_tag="ACHOTW_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843742.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAGG"
BASE COUNT         9163 a         9381 c        10152 g         9178 t
ORIGIN      
        1 gtcttcgtat acaaaaacac attcaaacaa tttttttaac aacatccatc atgcatttaa
       61 gaaaaaagat tggatttcga attatgatag cctgttaact ttgagggagt tctttcgttg
      121 cgcaactcag attgataaaa gtagttatca ggtgctttca tcaaaaaatg aaactgttaa
      181 cgctatggat aagtttctta tcagtttcag ccttaaagat aacggtgccg aatatacaat
      241 gacattgcga ggcagtggat ttgaatacga agaaatcccc ataacaataa atgagtataa
      301 ctcattcatg gatttcaaga accgtgaatt tccgttagaa caaaacagac gattgtatgc
      361 ctgggatatt ctgcagaaaa aacagtctga cataccgaaa aggattaaag gttatattca
      421 ccaggcgatt ggtgacgtgt ctttaggtta tgctttattg gatgatatag taagcaaatt
      481 aaaacggggt aaatttgaac tacaagggcc aggaggtgga attaaacagt gcgatggatg
      541 gtatatttac gaaaaaataa ttgatgataa ttttgccata gtcatagaat ctttgggctt
      601 tgcgcttaaa atctatggag gggatgaacg ttttcgtaat ggttcctctg ttgtcttgga
      661 ggatgaagat tactctctta tttataattt tctggttaat gcgggttgcc aacaagtcga
      721 attagccgaa caagtggatg cgatagtaag tgcgaatctg gccgcagata gtgatattac
      781 caaagaaaag atttgcgaga aatataaatc gaccattgag gcgtttaaaa aagagcaact
      841 agcgttacca gtattagttc ggcgtaagaa ctcagagact taattcccct atcccataga
      901 taacgatagg ggaaaattat ttttgcagaa tttatgttat tgccattatt tgagcaactt
      961 ttgcagcagg tccatcactt gctgcacctc ttcctgagtg agcgccccgt ctttggccca
     1021 ttgcacgcga ccgtctttat ccagcaccac tacagcggaa ctttcttcat ccagctgcca
     1081 ggcaccgcgc gcgacgccat tgctatcgac aataaactgc gaccagggat aaagcttttt
     1141 attactctcc agactgctgc gcacaaacat gccggaaccc ggaatcgcgt cgtcggtgtt
     1201 aacaatggtg gtggtctggt aacgatcgtg cggtaacttc gctgatttaa tcgcttcaat
     1261 cagcgtcgcg tttttctctt ttgcagaggt gcgaccagca atatgttgca gtactcgcac
     1321 ttttcccact aactgcgcgc tgttccaggt tttgtagcta aactgatctt tatcaagcac
     1381 caactcgccc cgatcggtaa tgccaatcgg cggcactcgc tgaccggttt cgaactgatg
     1441 tgcggaagcc atcatcggca acagcaggca ggtgagtgcc agaatcttgc gtagggtcat
     1501 ggtgtttcct tcttatgata tgcaggtgat ccgaccactt gggtctgagt tttaatcata
     1561 tgtgctattt atcgaatttc ccgcaagtgt gatgccagtt tgcggtcaag cgcacaaatc
     1621 atatgaaaaa tgaatgctta tactgaagac cgcgcttcgg taaaaagata attctgaata
     1681 attgtaacct ttaggtaaaa aaagttatac gcggtggaaa cattgcccgg atagtctata
     1741 gtcactaagc attaaaattt gcgcctcata atagttgggc cgattgtggc accgcacagg
     1801 cgtaatactc agcaggagat aacaatgaaa attttccaac ggtacaaccc acttcaggtg
     1861 gcgaagtacg taaagatcct gttccgtgga cgattataca tcaaggacgt tggcgctttt
     1921 gaattcgata agggtaagat tcttatccca aaagtgaagg ataagctgca tttgtcagtg
     1981 atgtccgaag ttaaccgtca ggttatgcgt ctgcaaacag agatggctta accaaagtgc
     2041 tatgcagtaa aaagtgctat gcagtaaaaa gtgctatgca gtaataagac ggctcctgat
     2101 tcaggagccg ttgatgttta tgggggttac gcgacgcttt cttctttatc tttagcgtca
     2161 ggcagttttg gcgaaagggc tgtggccttg ctgtcgatcc gggtcaccag cagctggtcg
     2221 atgcggtagt tatcgatatc caccacttca aatttgtagc cggcgaattt caccgaatcg
     2281 gtgcgtttcg ggatcttacg cagcataaac atcataaagc cgccgatggt ttcgtagttg
     2341 cccgactgcg ggaactcgtc aatatccagc acgcgcatga cgtcgtcaat cggagtgccg
     2401 ccgtcaatca gccatgagtt ctcatcacgg gcgacaatct gctcttccag tccctgaccg
     2461 accagatcgc ccatcagcgt ggtcatcacg tcgttgaggg tgatgatccc cactaccagc
     2521 gcgtactcgt tcatgatcac cgcgaagtct tctcctgcgg ttttaaaact ttccaacgcc
     2581 tctgaaaggg ttaacgtatc cggcacaatc agcgtgttgc gaatttgtac gccgctgttc
     2641 agtgccaggc tttggttagc cagcacgcgg ttcagcaggt ctttagaatc gacatagccg
     2701 atgatgtggt caatatcttc attacagacg aggaacttag agtgcggatg ttccgccacc
     2761 ttatttttca gactttgctc atcttcgtgg agatcaaacc aaatcacgtt ttcacgcggt
     2821 gtcattgaag acggaacggt acgggattcc agctcaaaga cgttttcaat cagctcgtgt
     2881 tcctgtttac gtaacacgcc cgccagcgca ccggcttcca ctaccgcgta gatgtcatca
     2941 gaagtgatgt catctttacg taccattggc agtttgaaaa tacggaagat tatgttcgcc
     3001 aggccgttga agaaccacac cagcggggtg caaacgtaca ggcagaagcg catcgggttg
     3061 atgatacgca aagccacagc ttctggcgca atcataccga tgcgtttcgg ggttaaatcc
     3121 gcaaacagga taaacatgcc agtcactaac gagaaagaga gaataaagct cagttgctca
     3181 gagagctctg ccgacatata gcgggagaac aggctgtgaa aagctggaga aaatgccgca
     3241 tcaccgacga taccgccgag aatcgccact gcgttcagac cgatttggac cacggtaaag
     3301 aacatgccgg gattttcctg catattcaga acgcgttggg catttatatt gccttcatca
     3361 gccagcagtt taagtttgat tttgcgtgag gcggcaagtg agatctcaga catcgagaag
     3421 aacgcactta cagcgatcaa gcagagtatg actaaaatac tgtttaacat atcttatccg
     3481 acctttcagg ccagatcctc ggaaggggaa gttgattaat ttgtgtgaaa tacacattga
     3541 aagccgattc gaagaaagtg aaccggcatt ttcagtggct agtatagcgt aaggtactgt
     3601 aaagccgcca gagggttaaa attcagacag ctgaaaaatg caaaactgcc tgatgcgcta
     3661 tgccgatcag gcctacgcag tccttgcaat atattgaatt tggacgattt tgtaggcctg
     3721 gcaaggcgtt tacgccgcat ccggcataaa caaaacgcac tttatcaaca atctacattt
     3781 gtacccgtaa cgctatgctt ccggtggcag acagacgcca attccgccaa ttccacagta
     3841 accgtacggg tttttatgca gatattgctg gtggtcatct tcggcataat aaaacggtgt
     3901 ggcgttagcg atttccgtgg tgatgtgacg atcgtcatcg gcggcaagca tcgccgcctg
     3961 aaaacgttcc agactggcgc gagctgcggc atcctgttct ggggtcagcg gataaatcgc
     4021 tgaacgatac tgcgtgccgt ggtcattgcc ctgacgcatt ccctgggcgg gatcgtgatt
     4081 ctcccaaaat acctgtagca actgctcata gctgatgacg gaaggatcgt aaacaatgcg
     4141 taccgcttcg gcatgaccag tatcaccgga gcacacttcc cgataagtcg gatttggcgt
     4201 atagccgccg gtataacctg cggcggtgct gtaaacgccg tgtaactgcc agaacagacg
     4261 ctccacaccc cagaaacaac ccatcgcaaa aatggcaatc tccattccgt caggtacatt
     4321 ggtcattgag tgaccgttaa ccgcatgcag cgtggctacg ggcatcgggg tgttacgtcc
     4381 aggcagggca tcggcggggg aaaccagatg ctttttatca aataaactca tggtgtcgct
     4441 ctcccgaaaa tcggtcattg gggttaaggt tgtaacaaga ggcgtatttg cacacaataa
     4501 ccaccgtgaa taggtctaaa gtaaaacata agaaatattt ggggtttagt ctgcttttta
     4561 atccatatta ctggattttt gttaagccgt ttaacggcgt tccaggggca ggaaaaaagg
     4621 atattcagga gaaaatgtgc gctatatccg acagttatgc tgtgtaagct tactctgctt
     4681 aagcggatct gccgtcgccg cgaacgtccg tctacaggtc gaggggttat cgggacagct
     4741 ggaaaagaac gttcgtgcgc agctttctac gattgaaagt gatgaagtga cgccagaccg
     4801 tcgctttcgc gcacgcgtcg atgatgctat ccgcgaaggt ctgaaagcgc tgggttatta
     4861 ccagccgacc attgaatttg atctccgtcc accgccaaag aaagggcggc aggtattgat
     4921 cgccaaagtc acgccaggcg tgccggtgtt aattggcggc accgatgtgg tattgcgcgg
     4981 gggcgcgcgg accgataaag actatttgaa attgctcgat actcgcccgg ctattggcac
     5041 ggtactgaac cagggcgatt atgaaaattt caaaaagtcc ttaaccagca ttgcgttgcg
     5101 taaaggttat ttcgatagcg aatttaccaa agcgcagctg ggcattgcgc tcggcctgca
     5161 taaagccttc tgggatattg attataacag tggcgaacgt taccgctttg ggcatgtgac
     5221 ctttgaagga tcacaaatcc gcgatgaata cctgcaaaat ctggtgccgt ttaaagaggg
     5281 cgatgagtac gaatcgaaag atctggcaga actgaaccgc cgactttctg ctaccggctg
     5341 gtttaactcg gtggtggtgg ctccacaatt tgataaagcg cgcgaaacga aagtattacc
     5401 attgacgggc gtggtttcgc cgcgaacaga aaacactatc gaaaccgggg tcggttactc
     5461 tacggacgtg ggaccgcgcg tgaaagcgac gtggaaaaaa ccgtggatga actcatacgg
     5521 tcacagtctg accaccagta ccagtatttc cgcgccggaa cagaccctcg acttcagcta
     5581 taaaatgccg ctgctgaaga atccactgga acaatattat ttggtgcagg gcggttttaa
     5641 gcgcactgac ctgaacgata ccgagtctga ctccactacg ctggtggctt ctcgctactg
     5701 ggatctctcc agcggctggc agcgtgccat taacctgcgc tggagtctcg accactttac
     5761 tcagggtgaa attaccaaca ccacgatgct gttttatcct ggggtgatga ttagccgcac
     5821 gcgttctcgt ggtggcctga tgccaacctg gggcgactcg caacgctact ctatcgacta
     5881 ctccaacacg gcctggggtt cagatgtcga tttctccgtt ttccaggcgc agaacgtctg
     5941 gatccgcaca ctgtacgatc gccatcgttt tgtgacacgc ggcacgctgg gctggattga
     6001 aaccggtgat ttcgacaaag taccgccgga tctgcgtttc ttcgccgggg gcgatcgcag
     6061 tattcgcggc tacaaataca aatctatcgc cccgaaatac gctaacggtg acctgaaagg
     6121 ggcctcgaag ttgataaccg gatcgctgga ataccagtac aacgtgaccg gaaaatggtg
     6181 gggcgcggtg tttgtcgata gtggcgaagc ggtaagcgat attcgccgca gcgactttaa
     6241 aaccggtacc ggggtcggcg tacgctggga atcgccggtc gggccaatca aactcgattt
     6301 tgccgtaccg gtcgcggata aagacgaaca cgggttacag ttttacatcg gtctggggcc
     6361 agaattatga gtttatggaa aaaaatcagc ctcggcgtgg ttatcgttat cttactgttg
     6421 ctgggatcgg tggcgtttct ggtgggcacc accagcggcc tgcatctggt atttaaagcg
     6481 gcggatcgct gggtgccagg actggatatt ggcaaggtca ccggcggctg gcgcgatctc
     6541 accttgtctg acgttcgtta tgagcagcca ggcgtggcgg taaaagcggg taatctgcat
     6601 ctggcggtcg gtcttgagtg cctgtggaac agcagtgttt gtattaatga cttggcgctg
     6661 aaagacattc aggtcaacat cgacagtaaa aaaatgcctc cttctgaaca ggttgaagaa
     6721 gaggaagata gcggtccgct ggatctctcc acgccgtatc ccatcaccct gacacgggtg
     6781 gcactggaca acgtcaacat caagattgat gacaccacgg tatcggtgat ggacttcacc
     6841 tccggcctga actggcagga gaaaaccctg accctgaaac cgacgtcgct gaaaggcctg
     6901 ctgattgctc tgccgaaagt ggcggaagtg gcgcaggaag aagtggtcga accgaaaatt
     6961 gaaaatccgc agccggagga aaagcctctc ggcgaaacac tcaaagatct cttttctcgc
     7021 ccggtattgc cggaaatgac cgacgtgcat ctgccactca acctgaacat tgaagagttt
     7081 aagggagagc aactgcgcgt gacgggcgat acggacatta ccgtgcgcac catgctgctg
     7141 aaagtgagca gcattgacgg caatactaaa ctggacgccc tggatatcga ttccaatcaa
     7201 gggatcctca acgccagcgg cacggcgcag ctgtcagaca actggccggt ggatatcacc
     7261 ctcaacagca cactgaacgt ggagccgttg aaaggtgaaa aggtgaagct gaaagtgggc
     7321 ggcgcgctgc gcgaacagct ggagattggc gttaatcttt ccggtccggt ggatatggat
     7381 ttacgcgccc agacgcgact ggcggaagcc ggattgccgc tcaacgtgga agtgaacagc
     7441 aaacagattt actggccgtt cactggtgag aagcagtatc aggcggatga tctgaaactg
     7501 aaacttaccg gcaaaatgac cgattacacg ctctctatgc gtacggcagt gaagggactg
     7561 gagatcccgc cagccaccat tacccttgat gccaaaggta atgaacagca ggtcaatctc
     7621 gacaaactca ccgtcgcggc gctggaaggg aaaactgaac tcaaggcgtt gcttgactgg
     7681 cagcaggcca ttagttggcg cggtgagcta acgcttaacg gcattaacac cgccaaagag
     7741 atcccagagt ggccgtcgaa actcaatggc ttgattaaaa cccgcggtag cctgtacggc
     7801 ggcacctggc agatggaggt gccagaactg aagcttaccg gtaacgtcaa acagaacaaa
     7861 gtgaacgttg acggcacgct gaaaggcaac agttatatgc agtggatgat cccagggctt
     7921 catctggaac tggggccaaa cagtgccgaa gtgaaaggcg agctgggggt aaaagatctc
     7981 aatcttgatg ccaccatcaa cgcgccgggg ctggataacg cgctgccggg gcttggcggt
     8041 acagcgaaag ggctggtgaa agttcgcggc acggtggaag cgccacaact actggcggac
     8101 atcaccgcgc gcggcctgcg ctggcaggag ctaaccgtgg cgcaggttcg cgtggaaggc
     8161 gatatcaaat ccaccgatca gattgcgggt aaactcgacg tgcgcgttga gcaaatttca
     8221 cagccggatg taaatatcaa cctcgtcacc ctgaatgcca aaggcagcga aaaacagcac
     8281 gagttacagt tgcggattca gggcgagccg gtttccgggc agcttaatct ggcaggaagt
     8341 tttgatcgca aagaagaacg ctggaaggga actcttagca atacccgctt ccagacgctg
     8401 gtcggcccgt ggtcgctgac ccgcgatatt gcgctggatt accgcaataa ggagcaaaaa
     8461 atcagcatcg ggccacactg ctggcttaac ccgaatgcgg aactgtgcgt gccgcaaact
     8521 atcgatgcgg gggccgaagg gcgtgcggtg gtgaatctca accgcttcga ccttgccatg
     8581 ctgaaaccgt ttatgccaga aaccactcag gccagcggta tcttcacggg taaagcggac
     8641 gttgcctggg acaccaccga agaggggctg ccgcagggca gtatcaccct ttcggggcgt
     8701 aacgtgcagg taacgcaaac cgtcaacgat gcggcgctgc ccgtggcgtt tcagacactg
     8761 aatctgacgg cggaattgcg taacaaccgt gccgaattgg gctggaccat ccgcctgacc
     8821 aataacggcc agtttgatgg acaggtgcag gtgaccgatc cgcaaggccg ccgtaatctt
     8881 ggtggcaacg tcaatatccg taacttcaac cttgcgatga taaaccccat ctttacccgt
     8941 ggggaaaaag cagcggggat ggtgagtgcc aacttgcgtc tgggtggtga tgtgcaaagc
     9001 ccgcagttgt ttggtcagct tcaggttacg ggtgtggata tcgatggcaa ctttatgccg
     9061 tttgatatgc aaccgagcca gcttgcggtc aactttaacg gtatgcgctc gacgcttgcc
     9121 ggtacagtac gaacccagca gggtgaaatc tacctgaacg gtgatgccga ctggagccaa
     9181 attgaaaact ggcgggcgcg ggtaacggcg aaaggcagta aagtgcggat caccgtgccg
     9241 ccgatggtac gaatggatgt atcgccagat gttgtattcg aggctacacc aaacctgttt
     9301 accctcgatg gtcgcgtgga tgtcccttgg gcgcgcatcg tggtgcacga tctgccggaa
     9361 agcgcagtag gcgtctccag cgatgtggtg atgcttaacg ataacctgca accggaagag
     9421 ccgaaaacgg cgtcgattcc gattaacagt aacctgattg tccacgttgg caacaatgtg
     9481 cgcattgacg cctttggcct gaaagcgcgg ctgacgggcg atcttaacgt cgttcaggac
     9541 aaacaagggc tgggcctgaa cgggcagatc aacatccctg aagggcgctt ccatgcctat
     9601 ggtcaggatc tgattgtgcg taaaggtgag ttactgttct ctggcccgcc ggatcaaccg
     9661 tatcttaata ttgaagctat tcgtaacccg gatgctacag aagacgacgt aatcgccgga
     9721 gttcgcgtca ctggtctggc ggacgaaccg aaagcggaga tcttctctga cccggcgatg
     9781 tcgcaacaag ctgccttgtc ttatttgcta cgtggacaag ggctggagag cgatcagagc
     9841 gacagtgcgg caatgacctc gatgctgatt ggtctggggg ttgcgcaaag tggccagatt
     9901 gtaggtaaaa tcggcgagac gtttggcgta agcaatttag cgctcgacac ccagggagta
     9961 ggcgactcct cccaggtagt ggtcagcggc tatgtattgc caggtctgca agtgaaatac
    10021 ggcgtgggta tatttgactc tatagcaaca ctcacgttac gttatcgctt gatgcctaag
    10081 ctatatctgg aagccgtgtc tggtgtagac caggcactgg atttgctcta tcagttcgag
    10141 ttttagcaat gcgaatattt gtctacggca gtttacgcca caaacaaggc aacagtcact
    10201 ggatgaccaa tgcccagtta ctgggcgatt tcagtatcga taactaccag ttgtatagcc
    10261 tgggccacta tccaggcgca gttccgggga acggaacggt acacggtgaa gtttatcgta
    10321 ttgacaacgc cacgctggcc gaacttgatg ccttgcgcac caggggcggt gaatacgcgc
    10381 gccagttgat tcagacgccg tacgggagtg catggatgta cgtttatcaa cgacccgtcg
    10441 atggattaaa gctaattgaa agcggcgact ggttagacag ggataagtaa ccctatgcat
    10501 acgccacctt cgggtggcgt tgttttttgc gagacgactc gcattctgtt ttgtaattcc
    10561 ctcacctttt gcttttctct ccgagccgct ttccatatct attaacgcat aaaaaactct
    10621 gctggcattc acaaatgcgc aggggtaaaa cgtttcctgt agcaccgtga gttatacttt
    10681 gtataactta aggaggtgca gatgcgtatt accataaaaa gatgggggaa cagtgcaggt
    10741 atggtcattc ccaatatcgt aatgaaagaa cttaacttac agccggggca gagcgtggaa
    10801 gtgcaggtga gcaacaacca actgattctg acacccatct ccaggcgcta ctcgcttgat
    10861 gaactgctgg cacagtgtga catgaacgcc gcggaactta gcgagcagga tgtctggggt
    10921 aaatccaccc ctgcgggtga cgaaatatgg taaagaaaag tgaatttgaa cggggagaca
    10981 ttgtgctggt tggctttgat ccagcaagcg gccatgaaca gcaaggtgct ggtcgacctg
    11041 cgcttgtgct ctccgttcaa gcctttaatc aactgggaat gacgctggtg gcccccatta
    11101 cgcagggtgg aaattttgcc cgttatgccg gatttagcgt tcctttacat tgcgaagaag
    11161 gcgatgtgca cggcgtggtg ctggttaatc aggtgcggat gatggatcta cgcgcccggc
    11221 tggcaaagcg tattggtctg gctgcggatg aggtggtgga agaggcgtta ttacgcttgc
    11281 aggcggtggt ggaataaggt gtgtttattt atcgcgggca taaaaaaacc cttactaacc
    11341 gaagcccggc gttcagggtt attacgccag aagaacttat ttattctttg cgcgctcgaa
    11401 ggaggcaacg atttcagctt tagcggcttc tgcgttttcc caaccttcaa ctttcaccca
    11461 cttgcctttt tcgaggtctt tgtagtgctc gaagaagtga gcgatttgcg ctttcagcag
    11521 ttcaggcaga tcgttaacgt ctttaatgtg atcgtattct ttgctcagct tgctgtgcgg
    11581 aaccgcaacc agtttcgcat cttcaccggc ttcgtcggtc attttcagaa cgccaaccgg
    11641 acggcaacgg atcacagaac ccggctgcag cgggtacgga gttgggacca gtacgtcaac
    11701 tgggtcaccg tccagagaca gggtgtggtt gatgtaaccg tagttgcacg gatagaacat
    11761 cgcggtggac atgaagcggt caacgaacag tgcgccgctc tctttgtcga tttcgtattt
    11821 gatcggatct gcgttagccg ggatctcaat aacaacgtag atgtcttccg gcagatcttt
    11881 acccgcaggg acgttgagta agctcatgtc tgtttccttt aaaaatatgt ggcaaacaag
    11941 tgccgagtat tatagccaac tcgcgccgaa tgtcttcgct tgttttcgtc ttcattttcc
    12001 tttttcgcca gttttaagac ggtatgcaga gcaggaaaat ccataaccgt agccgcattt
    12061 ttcatagtga gatgaaagcg attacaaact tgtgattaac gttttattta cttttttgaa
    12121 gtgtgatgta acgcaatccg ttacataacg aattgtctat agttttttcg cgaacatctt
    12181 ttcaccaata ataattactc cgacgaggac aaccctatgt ggaaacgctt acttgtagtc
    12241 tctgcagtct cggcagccat gtcgtctatg gcgttggccg ctccattaac cgtaggattt
    12301 tcgcaggtcg gatcggaatc cggctggcgc gccgcagaaa ccaatgtggc gaaaagtgag
    12361 gccgaaaaac gcggaattac gctgaaaatt gccgatggtc agcaaaagca ggaaaaccag
    12421 attaaagcgg tacgttcctt cgttgcacaa ggggtggatg cgatctttat cgctccggtg
    12481 gtcgcgacag gttgggaacc ggtattaaaa gaggcgaaag atgccgaaat cccggtcttc
    12541 ttgctcgacc gttccatcga tgtgaaagac aaatctctct atatgaccac cgttactgcc
    12601 gacaacatcc tcgaaggtaa gttgatcggt gactggctgg taaaagaagt gaatggcaaa
    12661 ccatgcaacg tggtggagct gcagggcacc gttggggcca gcgtcgccat tgaccgtaag
    12721 aaaggctttg ccgaagccat taagaatgcg ccaaatatca aaatcatccg ctcgcagtca
    12781 ggtgacttca cccgcagtaa aggcaaagaa gtcatggaga gctttatcaa agcggaaaac
    12841 aacggcaaaa acatctgcat ggtttacgcc cataacgacg acatggtgat tggtgcaatt
    12901 caggcaatta aagaagcggg cctgaaaccg ggcaaagata tcctcacggg ttccattgac
    12961 ggtgtaccgg acatctacaa agcgatgatg gatggcgaag cgaacgccag tgttgaactg
    13021 acgccgaaca tggcgggacc cgctttcgat gcgctggaga aatacaaaaa agacggcacc
    13081 atgcctgaaa agctgacgtt aaccaaatcc accctttacc tgcctgatac cgcaaaagaa
    13141 gagttagaga agaagaaaaa tatggggtat tgagggttgc tatgcctgat gccgattcgt
    13201 aggccggata aggcgctcgc gccgcatccg gcgatggtgc actgaagcct gatgcgacgc
    13261 ttaccgcgtc ttatcatgcc tactgggagc acgctttaca ccgggggaaa ccatgacgac
    13321 cgaccaacac caggagatcc tccgcaccga aggattaagt aaatttttcc ccggcgtcaa
    13381 agcgttagac aacgttgatt tcagcctgcg ccgtggcgaa atcatggcgc tgctcggtga
    13441 aaacggggcg ggaaaatcaa cgctaatcaa agcattaact ggtgtatacc acgctgatcg
    13501 cggcaccatc tggctggaag gccaggctat ctcaccgaaa aataccgccc acgcgcaaca
    13561 actcggcatt ggcaccgtct atcaggaagt caacctgcta cccaatatgt cggtcgctga
    13621 taatctattt ataggccgcg aacccaaacg cttcggcctt ctacgccgca aagagatgga
    13681 aaagcgcgcc accgaactga tggcatctta cggtttctcc ctcgacgtgc gcgaaccgct
    13741 caaccgcttt tcagtcgcga tgcagcaaat cgtcgctatt tgccgggcta tcgatctctc
    13801 tgccaaagtg ctgatcctcg atgaacccac cgccagtctc gacacccagg aagtggagtt
    13861 actgtttgac ctgatgcgtc agttgcgcga tcgcggcgtc agcctgatct ttgtcactca
    13921 ctttctcgat caggtctatc aggtcagcga tcggatcacc gtcttacgca acggcagttt
    13981 cgtaggctgt cgggaaacgt gcgagctacc gcagatcgaa ctggtaaaaa tgatgctggg
    14041 gcgcgagctg gatacccacg cgctacagcg tgccgggcga acattgttga gcgacaaacc
    14101 cgttgccgcg ttcaaaaatt acggcaaaaa aggaacgatc gcaccgtttg atctcgaagt
    14161 acgccccggc gagatcgtcg gtctggctgg attgctggga tcaggacgta ccgaaaccgc
    14221 cgaagtgatc ttcggtatca aacctgctga cagcggcacg gcgttgatca aaggcaaacc
    14281 gcaaaacctg cgatcgccac atcaggcttc ggtacttggc attggcttct gcccggaaga
    14341 caggaaaacc gatggcatca tcgctgccgc ctcggtgcgg gaaaatatca tcctcgctct
    14401 ccaggcccgg cgcggctggc tacgtcccat ttcccgcaaa gaacagcaag agattgccga
    14461 acgctttatc cgccagcttg gcattcgcac accttcaact gaacaaccga ttgaatttct
    14521 ctccggcggc aatcagcaaa aagtgttgct ttcacgttgg ctactgaccc gaccgcaatt
    14581 tctgatcctc gatgagccaa cccgcggcat tgatgttggt gcccacgccg agatcatccg
    14641 cctgattgaa acgctatgcg ccgatggtct ggcgctgctg gtgatctcct ccgaactgga
    14701 agaactggtg ggctatgccg accgggtgat catcatgcgc gatcgcaaac aggtggcgga
    14761 gatcccgctg gcagagcttt ccgttccggc gatcatgaat gccattgcgg cgtaaggaga
    14821 acagtgtgat gcctcaatct ctcccggaca ctacgccgcc aaaaaggcgc tttcgctggc
    14881 caacgggaat gccgcagctg gcggcactgt tgctggtgct gctggtcgat agcctggtgg
    14941 ccccgcattt ctggcaggtg gtgcttcagg atgggcgttt gttcggtagc cctatcgaca
    15001 ttcttaatcg cgccgccccc gttgcgctac tggcgattgg tatgacgctg gtgatcgcaa
    15061 caggtgggat tgatctctcc gtaggggcgg tgatggctat cgccggagcc acaacggctg
    15121 cgatgacggt cgcgggattc agcctgccga ttgttttgtt aagcgccctg ggcaccggca
    15181 tcctggcggg attgtggaac ggcatactgg tagcgatcct caaaattcag ccgtttgtcg
    15241 ccactctgat cctgatggtc gccgggcgcg gcgtggcgca actgatcacc tccggacaga
    15301 tcatcacgtt taactcgccg gatctctcat ggttcggcag tggatcgctg ttgttcctgc
    15361 caacgccggt cattatcgcg gtgctgacgc ttatcctgtt ctggctgttg acccgcaaaa
    15421 cggcgctggg gatgtttatc gaagccgttg gtatcaacat tcgggcggca aaaaatgccg
    15481 gggtaaacac gcggatcatc gtcatgctta cctacgtgtt gagcgggctg tgtgcggcga
    15541 ttgcgggcat tatcgtggcg gcggatattc gcggtgccga tgccaacaac gccggattat
    15601 ggctggagct ggacgccatt ctcgcggtgg tgattggcgg tggttcgctg atgggcggac
    15661 gttttaacct actgctttcg gtggtggggg cgctgattat tcaggggatg aacaccggaa
    15721 ttttgctttc gggctttccg ccagagatga accaggtggt gaaagcggtg gtggtgcttt
    15781 gcgtgctgat tgtccagtcg caacgcttta tcagtctgat taaaggagtg cgtagccgtg
    15841 ataaaacgta atttgccact gatgatcacc atcggcgtct ttgtgctggg ttatctctac
    15901 tgcctgaccc agtttcccgg ttttgcttcc acaagagtga tctgcaatat cctgaccgat
    15961 aatgcctttc ttgggataat cgccgttggc atgacctttg tgatcctctc cggtgggatc
    16021 gatctctccg ttggttcggt gatcgccttt accggcgtgt ttctggcaaa agtgattggc
    16081 gatttcggcc tctcgccgtt gctggcattt ccgctggtgc tggtaatggg ctgtgccttt
    16141 ggcgcattta tggggctttt gatcgacgcc ctgaagatcc cggcatttat cattactttg
    16201 gcaggaatgt tctttttgcg cggcgtcagc tacctcgttt cggaagagtc gatcccgata
    16261 aaccatcctg tttatgacac gctctcaagc cttgcatgga aaattcctgg cggcggtcgc
    16321 ttaagtgcga tggggctgct gatgctggcg gtggtggtta tcggcatatt cctcgcgcat
    16381 cgtacccgct ttggtaatca ggtgtacgcc attggcggca atgcgacgtc ggcaaacctg
    16441 atggggattt ccactcgcag cacaactatt cgcatttata tgctctctac cggactggcg
    16501 acactggcgg ggattgtctt ctcgatttat acccaggccg gatacgcgct ggcgggcgta
    16561 ggtgtggaac tggacgctat cgcctcagtg gtgattggcg gtacgctttt gagcggtggc
    16621 gttggaacgg tattagggac gctttttggc gtggcgattc agggactgat tcagacttac
    16681 ataaactttg atggtacgct gagctcatgg tggacgaaaa tcgccatcgg tattttgttg
    16741 tttattttta tagcattaca gcgtggatta acggtgctgt gggagaatcg tcagagttcg
    16801 cctgtgacaa gagtcaacat tgcgcagcaa taaaatcgcc tctccgtgtg gagaggcgca
    16861 ggagattacg cgtccgggaa ctcacagata aagcgttcga catcttcaac catatggtcg
    16921 ttgccgacaa agaatgaacg gcgctggtgc agggtttccg ggataatatc cagaatacgc
    16981 tctttgccat cgctcgcttt accgcccgct tgttccgcca ggaatgccat cgggttgcac
    17041 tcatacagca aacgcagttt gccgtccggg tggctggcgg tgcttgggta gagataaata
    17101 ccgcctttca gtaggttacg gtggaaatcc gcaaccagtg agccgatata acgtgaggta
    17161 tacgggcggt tggtggattt atcttcttcc tggcagaatt taatgtactt cttcaccccg
    17221 ttcggaaact taatgtagtt tccttcgttg atggagtagg ttttgccttt ctccgggaag
    17281 cgcatccgtt cctggcacag gcagaaaacg ccgagcgaag gatcgtaagt aaaagcgtga
    17341 acaccgcatc cggtactgta aaccagcatg gtagaggagc cgtataccac gtaacctgcc
    17401 gcaacctgtt tgttaccagg ctggaggaaa tcttcttccg ttaccggcgt gccgacgggc
    17461 gtaactcggc ggtagatgga gaaaatggta ccgacagaga cgttaacatc gatgttggac
    17521 gagccatcca gtgggtccat cagcaccacg tattttgcat gttcacagcc ttcaaagaca
    17581 acaatctcat cttcttcttc agaggcaatg cctgcaacga tatcgcgtgc tttcagtgcg
    17641 gctttcagtt tttcattagc gaacaagtcg agtttttgct gaacctcgcc ctgcacgttc
    17701 tcagcaccgc tggcacccag gatatcaacc agtcctgctt tgttgatatc gcgatggata
    17761 atcttggcgc ccagttttat tgccgacagc aaagcagtga gctcaccggt agcatgagaa
    17821 aactcgtgct gcttttcgac aataaattca cctaacgttt tcataaaact ttccctgcaa
    17881 tgtttatgga gtaaagcgac cgcaacaatc ttaacaaata atctcaatgt tgcgctcagg
    17941 tgaatcgcgc cagcaaatta cggattatcc tgaaatgcgt ttctcacttg cccgacatat
    18001 gcgtaaaatg agcggcagat taaaaaagga tagtgacgca tgcgcattca tattttagga
    18061 atttgtggca cgtttatggg cggtctggcg atgctggcgc gccagttagg ccatgaagta
    18121 acgggttcgg acgccaatgt gtatccgccg atgagcacct tacttgagaa gcaaggcatt
    18181 gagctgattc agggttacga tgccagccag ctcgacccgc agccggatct ggtgattatt
    18241 ggcaacgcca tgacccgtgg aaatccgtgt gtggaagcgg tactggaaaa aaacatccct
    18301 tatatgtcag gtccacagtg gctgcacgat tttgtgctgc gcgaccgctg ggtgctggcc
    18361 gttgccggta cacacggcaa aaccaccacc gcgggaatgg cgacttggat tctggaacag
    18421 tgtggttaca aaccgggctt tgtaatcggc ggtgtgccgg ggaactttga ggtttcggcg
    18481 cgtctgggcg aaagcgactt ctttgttatc gaagcggatg agtacgactg cgccttcttc
    18541 gacaaacgct ctaaatttgt ccattactgc ccgcgtacgc tgatcctcaa caaccttgag
    18601 ttcgatcacg ccgatatctt tgacgacctg aaagcgatcc agaaacagtt ccaccatctg
    18661 gtgcgtatcg ttccggggca gggtcgtatt atctggccgg aaaatgacat caacctgaaa
    18721 cagaccatgg cgatgggctg ctggagcgag caggagctgg tgggtgagca gggtcactgg
    18781 caggcgaaaa agctgaccac cgatgcttcc gaatgggaag ttttgctgga tggcgaaaaa
    18841 gtgggcgaag tgaaatggtc gctggtaggc gaacataata tgcacaatgg cctgatggcg
    18901 attgcagcgg ctcgccatgt tggtgtagcg ccggcagatg ccgctaacgc gctgggttcg
    18961 tttattaacg ctcgccgccg tctggagttg cgtggtgaag cgaatggcgt cacggtatat
    19021 gacgattttg cccatcatcc gacggcgatt ctggcaacgc tggcggcgct gcgtggcaaa
    19081 gttggtggta cggcgcgcat tattgctgtg ctggagccgc gctcgaatac catgaaaatg
    19141 gggatctgca aagacgatct ggcaccttca ttaggtcgtg ccgatgaagt cttcctgctg
    19201 caaccggcgc atattccgtg gcaggtggca gaagtggcag aagcctgcgt tcagcctgca
    19261 cactggagtg gcgatgtgga tacgctggca gatatggtgg tgaaaaccgc tcagcctggc
    19321 gaccatattc tggtgatgag caacggcggt tttggtggga tccatcagaa actactggat
    19381 ggtctggcga agaaggcgga agctgcgcag taattcggcc tcagcctgag atagtgtgcg
    19441 taggccggat aaggcgttca cgccgcatcc ggcatttgag catagtgcct gatgcgacgc
    19501 ttgatgcgtc ttatcaggcc tacaggtgca ccgcatccgg caaaccatac tcacatcaac
    19561 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
    19621 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag agtacgcagt
    19681 tgctgacgat cggcatcggg ccacagattt aatacctcag cgatggcgtc atcaccttga
    19741 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
    19801 cggtttttca gcttatccag cgcctgacgg atcggttcta catcgcgctg gcgcagcatc
    19861 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
    19921 atagccgcgc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
    19981 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
    20041 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
    20101 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
    20161 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
    20221 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
    20281 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
    20341 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagcg
    20401 atggcgcgct ggggatcact gtttatcacc agaaccgcaa aggtagcgca tcatccaccg
    20461 atttaagccc gcaggccatt gcccgtactg tacaggcggc gctggatatt gcccgttata
    20521 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccgg
    20581 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
    20641 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
    20701 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
    20761 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
    20821 gtgattacgc ctacaccatt ggccgcgcga tgagcgattt gcagacgcca gagtgggtcg
    20881 gggccgattg cgctcgccgt actttatcgc gtctgtcacc gcgtaaactc tccaccatga
    20941 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttgggcat ctggtggggg
    21001 cgatagcggg tggatcggtt taccgtaaat ctaccttcct gctggattcg ctgggtaaac
    21061 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcat
    21121 cgacgccatt tgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
    21181 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
    21241 gacatgcggg cggtattcac aactggcgga ttgccggaca aggtctaagc ttcgagcaga
    21301 tgctcaaaga gatgggcacc gggctggtgg tgacggaatt gatgggccag ggcgtgagtg
    21361 caattaccgg tgattattcc cgtggtgcgg cgggcttctg ggtagaaaac ggcgaaattc
    21421 agtatccggt tagcgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
    21481 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
    21541 cggagatgaa aatcgccgga cagtaaaaaa actggcgcga cctgccgcgc tataaactaa
    21601 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
    21661 gtactcgcag tacgtgaccc ggtcgaatga gcgtagtcgt cgatgaggca gtgtgaagga
    21721 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgaaaaag cctgttagct attcttgcag
    21781 tctcctcgtt ggtattcagt tcggcgtcgt ttgctgctga tcttgaagac aatatggaaa
    21841 ccctcaacga caatttaaaa gtggtcgaaa aagccgataa cgcggcgcaa gtcaaagacg
    21901 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
    21961 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
    22021 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaagtaaaa gaagcgcagg
    22081 ctgctgcaga gcaactgaaa acgacccgca attcatatca caagaagtat cgttaattcc
    22141 tcatttccct gttgcctgca ctcaggtaac agggaactta tcatcgcaaa tgatgcacca
    22201 cctgattgct gctgccgcgc cagatcaggg aagggtcttt taaatcctgc acaaatttgc
    22261 cgtcgaccag cacgttaatc aaatcaacaa cctgcatttg cgcagcgttg agttcgtcga
    22321 gtttatagcc tgtccacacc cagatgtctt tacccggaca ctcggcgcgg atgcgttgta
    22381 ccagtttcag aatatccggc acgttttgcg gatgcagcgg atcgccgccg gagagggaaa
    22441 taccctggcg tttgatacga gtgtcattca gatcgttaat gatctggtct tccattgctt
    22501 tagtaaatgg ctgaccggaa tttacccgcc atgtgctttt gttatagcaa ccggggcatt
    22561 catgaacaca cccggagaca aacagggtgc agcgagtgcc gggaccgttg acgatgtcga
    22621 caggatagta ctgatgataa ttcattattt cgcctggctt atcagtccgc gtgtaggctg
    22681 tatcaggcaa tcaacgcctg atgcgacgct gtcgcgtctt atcaggccta cagccgttgc
    22741 caaatgtagg ccggataagg cgtttacgcc gcatccggca atcaacgcgg gatgcgacgc
    22801 tttcacatca catccggcat ttgcttagtg cagatcaacc tatctgccca tttcccaaat
    22861 gtttaacgcg gcgcttaact tcttcctgct tgccggcgtt aaacggacgt gcatccgggc
    22921 tacctaaata tccgcacacg cggcgagtta ccgacacacg ggaggcgtca tggttaccac
    22981 atttcgggca ggtgaagcct ttgctggtgc actcgaactc accggtaaag ccacactcgt
    23041 agcactcatc aatcggtgta ttggtgccgt aatacggtac atgctgatag ctgtaatccc
    23101 agacatcttc cagcgccttc aggttgtgct gaatgtttgg atactcgccg tagcaaatga
    23161 aaccaccgtt cgccagcggc gggtaaggcg cttcaaagtc gatcttgtcg tacgggttca
    23221 ccttcttctc cacatcgagg tggaaactgt tggtgtagta acctttatcg gtcacgcccg
    23281 gcaccacgcc aaactcagca gtatcgagac ggcagaagcg gtcgcacagg ttttcactcg
    23341 gcgtgctgta gagactgaaa ccataacccg tttcttcttt ccactgatcc actgcctgac
    23401 gcagacgttc aacaatggcg atacctttcg cgcggagctg ctcgttgtcg taaacatgtt
    23461 cgccgccgaa cagcgcgtta atggtttcgt ggatgccgat gtaacccagc gaaatagacg
    23521 cacgaccgtt tttgaagatt tcagaaatat catcgtcagc attgagacgc acgccacaag
    23581 caccttccat atagaggatc ggggccacgc gcgctttcac gccttcgaga cgagcgatac
    23641 gggtcatcag agccttacgt gccagcacca gacgttcatc cagcagcttc cagaaggtgg
    23701 cttcatcgcc ttttgcttcc agagcaatac gcggcaggtt caggctgatc acgccgaggt
    23761 tgttacgacc atcgtggatc tgctcgccgt tttcattttc ccacacgccg aggaagctgc
    23821 ggcagcccat cggggtttta aacgaaccgg tgactttcac tacctgatcg tagttcagga
    23881 tatccggata catgcgcttg cttgcgcact ccagcgccag ctgtttgatg tcgtagttcg
    23941 gatcgccttt tttatggttc agaccatcgc gaatcgcaaa caccagtttc gggaacaccg
    24001 cagttttacg gtttttaccg aggcctgcga tacggttacg caggatagat tcctgaatca
    24061 ggcgcgactc ccagctggtg cccagaccaa aaccaaaggt tacaaacggc gtctgaccgt
    24121 tggcggtgtg cagggtgttt acttcgtact ccagcgactg gaaggcatcg tagcactctt
    24181 taatggtacg agagttagcg tagccttcgg cgtccgggat gttccactct tctgcggtct
    24241 tgcgatgttt gttgtagctg gcggtgacaa acggtgccag cacttcatcg atacggttaa
    24301 tggtggtgcc gccataaata tggctggcaa cctgagcaat aatctgcgca gttaccgcgg
    24361 ttgccgtaga gatcgacttc ggcggttcaa tctcggcgtt ccccatctta aagccctggg
    24421 tcagcatgcc tttcaggtcg atcaacatgc agttgaacat cgggaaaaac ggtgagtaat
    24481 cgagatcgtg atagtgaata tcgccacgct catgtgcctg caccacgtca cgcggcagca
    24541 ggtgctgacg tgcatagtgt ttagccacga tcccggccag caggtcgcgc tgggttggaa
    24601 tcaccttgct gtctttgttg gcgttttcgt tgagtaacga ggcgttggtc tgctcgacca
    24661 gaccacggat ctcctgattc aggcgaccgc gtttttcacg ttcaatgtcg cgatcgtgac
    24721 ggtactcgat gtaagcacga gccagttgtt tgtatggacc tgacatcagc tggttttcaa
    24781 cagcggtctg gatctcattg atatccacct ggttgcggcc ctgcatctgc tcgctgacaa
    24841 ctgcagcaac agtggcgcaa taatcggcat catcgacttc cgctgcttta gctgcacgca
    24901 gaatcgcttc tttgatgcgc tctgatttaa acggcacttt gcagccgtct cgtttcatca
    24961 catgcggtgt catgatcgct ccatattttt aagaacaggt tatccacaga aattgggaaa
    25021 ggcgttttcc ggttttttcg tttcgttttc cggtgctttc cgcaatcgcc atccgcttat
    25081 ccacaacgcc gcaccttttt cgtggggagc tgtagtagca attatagtcg attgatacaa
    25141 catattgggt tgggacgcat tttaaagtct atatatagtg ctttgcatca aggatgtttg
    25201 aacttttttt gatgtagctc aaagtaaaaa gcagagcgta cggatgacgg gcgctacagc
    25261 gttatgtaaa ttttttaatg aatttgctgg ttgaaaaatc aacaaaaaca acatgctgac
    25321 agacaaaacc ccggaatgac cggcattacc ggggcttagg gaagatttac ttctgtaacc
    25381 accagacagc ctcaaaaggc cgtaaagtca tggcacaggg ttgtggtgag gcttcttcgt
    25441 agttatgcat cacaagctgc cagttgccgc gcatttgccc tggctgccag ggttggatct
    25501 cacggctaag gttggcaatg accagcaagg tttgcccctt ccattcacgg cgatagcacc
    25561 acaatacagg gctgtttggc agcagatcct ggtaattgcc ccatgtcagg atggcttcct
    25621 gcttacgcag tgcgattaac ttttggtagg tgtaaaacac cgaggactca tcggccagcg
    25681 cggcttctac gttgatttgt tgatagttat cgcccaggcc aatccacggt tcgccagccg
    25741 taaacccggc attatcgccg ttgctccatt gcatgggcgt gcggctgttg tcacgggatt
    25801 tactggcgag gattgccaat aactcgtcgg catcacgccc atcgttgcgc agctcggcaa
    25861 acatattgag gctctctacg tcgcgatagt cagtaatgcg cgtgaaatgc gggttggtca
    25921 tgccaatctc ttcgccctgg tagatatacg gcgttccctg catgccatgc agcaccatcg
    25981 ccagcatttt tgccgcaggc acgcggtatt caccttcatc accaaagcga gaaacaatgc
    26041 gcggctgatc gtggttacac cagaacaagg cattcaatgc tacgttgtgc attccttgtt
    26101 gccagtggcg gaacaatgtt ttcaacgcca caaagtcagg tttagccagc gtccattttt
    26161 caccaccggg ataatcgacc ttcaggtgat gaaaattaaa ggtcatcgac aattcactgc
    26221 ctgtcagtgc cgcgtatcgc tggcaatgct caaggctggt ggaggacatt tcacctacgg
    26281 tcattaaccc gcgtggcgta aacacatcgc ggttcatctc gtgcaaaaac tcgtgtgctc
    26341 gtggcccgtc ggtgtagaag cgacgcccgt cgccatccag gtcatcaggg aaacgcgggt
    26401 ctttggagat cagattcacc acatccaggc gcaacccgtc gaccccacga tcggcccaga
    26461 actcacagac ttttttcagc tctgcgcgta ccgctggatt ctcccagttg agatccgcct
    26521 gttctggtgc aaagagatgc aaatagtact gttcgctttc cgcatgccag cgccacgcac
    26581 taccgccaaa ttttgaacgc cagttgttcg gtggcgtttc tggttcgcca tcgcgccaga
    26641 tataaaactg gcggtaaggg ctttctttgt tcagcgcctc gcgaaaccag gcatgttggg
    26701 tagaggtatg gttaaacacc atatcgagaa tgatacgaat cccgcgcgat tttgcctgcg
    26761 tcaccagttc gtcaaaatcg tccagcgtgc cgtaggtggg atcaatcgcc gtatagttcg
    26821 ctacatcgta accgttatcg acctgcggag agacataaaa gggggttagc cagatggcat
    26881 caacgcccag tttatgcaga tagtccaggc gttggataac gccacgtaaa tcgccggtgc
    26941 cgctacccgt ggtgtcctga aaactctttg gataaatctg gtagataacg ccgttttgcc
    27001 accagtgggg aagattagtc attacgttat tcctgcaaat gcgagggggc gcaattgcgc
    27061 cccgaagaaa attaaacaat gtccagcgtg cccaggcggt atttccgctg atagataaac
    27121 gaggtgagta caatcgggat gatgatggcg atagccattg ccagcgcaaa cacctgccag
    27181 tagctcggtt gaatcgagag aattcccggc aggccgccta cgccgatgcc attcgccata
    27241 acgccgttca ggccgcatag caatcctgcc agaccagaac caatcatcgc gcacagcatc
    27301 gggaagcgat atttcaggtt gatgccgtac attgcaggct cagtgacccc aagccaggcg
    27361 gagatcgccg caggcacgga gatctcgcgt tcattgtgct tgcggctgga aatgataatg
    27421 cctatcacgg cggagccctg agcgatattc gacagcgcaa tcagcggcca cactggcgta
    27481 ccacccatgc tttgaatcat ctgcaaatca atagcaagcg tggtctggtg tacaccggtg
    27541 atcaccagcg gggcgtacag gaagccaaac aatgccgcgc caatcggagc aaagctgccg
    27601 gtcatcaggt gacgtaccgc aaaggcaacg ccatcgccaa tcatgcgacc aaacggacca
    27661 atcagcgcat gggcgaggaa caccgcgagg atcagcgaac agacgggcac caccaccaga
    27721 tagaggtaat ccggcacgat gcgtttaagg cgagtttcaa taacgcccag cgccagcccg
    27781 gctaacagtg ccgggatcac ctgcgcctga tagcccactt tggcgatgct gaacatgcca
    27841 aagtcccaca cttccggcag ctgctgcccg agcagataag cgttcatcag ttggggggaa
    27901 accagtgtca cgccaagcac gataccaagg atcggcgtgc cgcccatttt tttcaccgct
    27961 gaccagcaaa tcccgaccgg caggtagaag aagatcgctt caccgatcaa ccacagaaaa
    28021 tcgtagatcg tttgcaggga agggtacatt tgcgccagcg tttgaccgtt gctcatgggc
    28081 aaatcgccga tcacattgcg aaaaccgagg atcaaaccgc cgctaatcaa cgcgggcagc
    28141 aacgggaaga agatctctgc gaaatgagag atcaactgct catgccattt catattctgc
    28201 cgggcggctt tttttacctg ctctttatca acctgcgcct gtccggttga cgctatcagt
    28261 gcttgatagt aatcacccac gttggtgcca atcaccacct gaaattgccc ggcattggtg
    28321 aaacagcctt tcaccatggg gagttgctca atttctttcg gtctggcatt ggccggttgg
    28381 ttgaggacaa agcgcaggcg agtaatacag tggctcaccg tcgcaatatt gccgcgcccg
    28441 ccgaccagtt caatcaaccg atcgatatcc gtttggttta ttttgctcat cataaagccc
    28501 catggcagat gacatttttg gttggctgca gaatatattg cgcggaaaaa tttaaaaacg
    28561 ggaacgttcc cgaaacgcag cgaagatcac aatttatcgt tcaggaaacg atcaggacag
    28621 gttggcgggg atgatgattt gttgcggttc gctgcgttcg gttacctgcg cgatcaactg
    28681 gcaagccgcc tggcgtccag cttcggcgta accaggatct acggtgacga tctccggatg
    28741 aaggaatttc attaacggcg tattaccgac gctcgccagt tgcaaggtgt cgatgcgttg
    28801 ctcttgcagg tatttacttg cgccaagtgc cagcgtgtcg gttgcgcaca gtaaggcggt
    28861 agtttcaggc gtaatcactt ttgctacgtt ctcatagcct tgcttcatag caagccctgg
    28921 cagggcggca acgggatgca gtttatgcgc tttgcagaac gccaggtagg cttcgtgacg
    28981 tcgcttaccg gttgtcacgt cactgtgcgg cacgccgaga taactgatat tacgatgccc
    29041 ctggtcatac agccgttgca tcaggatctt gattgcccct tcgtcgtcat aacagaccga
    29101 agcaaagcct tttgcgtcac gcgccagcag aaccagcgat gactgccagt gggctaacat
    29161 ttcttctgtt atgccagtaa aaccgaacag cactacgccg tcgatattac gccgtttcag
    29221 cacccccaaa tgttcggcaa ctaattgcgg ggaaaactga ctttccatca tgattgggtc
    29281 gtaaccttgt tcatagaacg ctggcagcat ggtttgaacg gcgagatttt ctgacaacga
    29341 atccagacgg gtaacaatga tggcgaccac tttatcgctt tgcccacgca tagcgcgcgc
    29401 agaacgggaa ggggaaaatc catgctgatt catcactgct tcaacacgct cgcgggtgcg
    29461 ctggctcacg ccgctttcgt tattcagcac ccgggaaact gtagatttcc ccacgccgct
    29521 taagcgcgcg atgtctttga tggtcagccg attttgcatc ctgttgtcct gtaacgtgtt
    29581 gtttaattat ttgagcctaa ctttacccgt gcattcagca atgggtaaag tctggtttat
    29641 cgttggttta gttgccagca ggtattatat cgccatagat gctacgaata ttattggatt
    29701 ctccttatta tttgcggcgc ttttttcact taccggaggt tatatggaac ctgatcccac
    29761 gcctctccct cgacggagat taaaactttt ccggtaagcc cgtcttttca cggcgttacc
    29821 ggatgcgtaa ggccgtgacg ttttaacgtc cctgctcagc tttattacct tcaggtaagg
    29881 cttcgccacg cctgaagaca tttctgtact gtttcagaca gtgcggaggg actccttatg
    29941 tttaaagaaa tttttacccg gctcattcgc catttacctt cccgtctggt tcatcgtgat
    30001 ccattgcctg gcgcgcagca gacagtgaat acggtggtcc cgccgtcctt aagtgcgcat
    30061 tgcctgaaaa tggcggtgat gcccgaagaa gaattgtgga aaacgttcga cacccatccg
    30121 gaagggttaa atcaggcgga agtggaatct gcccgcgaac aacatggtga aaataaatta
    30181 cccgcacaac aaccgtcgcc gtggtgggta catttatggg tctgctatcg caaccccttt
    30241 aatattttac tcaccattct cggcgctatt tcttacgcca cggaagattt atttgccgcg
    30301 ggcgttatcg cgctaatggt cgctatttca acgttgctga actttattca ggaagcacgt
    30361 tccactaaag cggcagatgc cctgaaagcg atggtcagca ataccgcgac ggtgctgcgc
    30421 gtaattaacg acaaaggcga aaatggctgg ctggagatcc cgatcgacca gctggtgccc
    30481 ggcgatatta taaaactggc ggcgggagat atgatcccgg cagatttacg tatcttgcag
    30541 gcgcgggatc tgttcgtcgc tcaggcgtcg ttaaccggtg agtctctgcc cgtggaaaaa
    30601 gccgctacca ctcgccagcc ggagcacagc aatccgctgg agtgcgacac gctgtgtttt
    30661 atgggcacca ccgtggtgag cggcacggca caagcaatgg tgattgctac aggtgccaat
    30721 acctggtttg gtcaactggc ggggcgtgtt agtgagcagg aaagcgagcc gaatgccttt
    30781 cagcaaggga tcagccgcgt cagtatgctg ctgattcgct ttatgctggt gatggcaccg
    30841 gtggtgctgt taatcaatgg ctacaccaaa ggcgactggt gggaagcggc gctgtttgcg
    30901 ctttcggtcg cggtaggcct aacgccggaa atgttgccga tgattgttac ctcgacgctg
    30961 gcgcgcggcg cagtaaagct gtcgaaacag aaagtgatcg tcaaacatct ggatgctatt
    31021 cagaactttg gcgcaatgga tattctgtgc actgataaaa ccggcaccct gacacaggat
    31081 aaaattgtgc tggagaatca taccgatatc tccggtaaaa ccagcgaacg cgtgctgcat
    31141 agcgcgtggt tgaatagtca ttatcagacc ggacttaaaa acctgctcga tacagcggtg
    31201 ctcgaaggta cggatgaaga gtcagcgcgc tcgctggcca gtcgttggca aaaaatcgat
    31261 gagattccgt ttgatttcga gcgtcgccgg atgtcggtgg tagtggcaga aaataccgag
    31321 caccatcagc tggtttgcaa aggtgcattg caggaaatcc tcaatgtgtg ttcgcaggtg
    31381 cgtcacaatg gcgagattgt gccgctcgat gacatcatgc tgcgtaagat taagcgggtt
    31441 actgatacgc tgaatcgtca ggggctgcgc gtggttgcgg tggcgacgaa atacctgcca
    31501 gcgcgtgaag gagattacca gcgggcggat gaatccgacc tgatcctcga aggatatatt
    31561 gctttccttg atccgccaaa agagacaacc gctccggcac tgaaggcatt aaaagcgagt
    31621 ggaattaccg taaaaatcct tactggcgac agtgagttag ttgcagcgaa agtgtgccat
    31681 gaagtggggc tggacgcggg agaggtggtc attggtagtg atattgaaac gctatctgac
    31741 gacgaactgg caaatcttgc acagcgcact acgctgtttg cccgcctgac gccgatgcat
    31801 aaagaacgca ttgtgacctt actgaagcgc gaagggcatg tggttggctt tatgggcgat
    31861 ggtattaatg atgcgcccgc cttacgcgct gcggatatcg gcatttctgt ggacggcgcg
    31921 gtagatattg cccgtgaagc ggctgatatc atcctgctgg aaaaaagcct gatggtgctg
    31981 gaagaggggg ttattgaggg acgtcgcact ttcgccaaca tgctgaaata catcaaaatg
    32041 acggcgagtt ctaacttcgg taatgtgttc agcgtgctgg tggcgagtgc tttcttgccc
    32101 ttcctgccga tgttgccgtt acacttgctt attcagaacc tgctgtacga tgtgtcacag
    32161 gtggcgatcc cgtttgataa cgtcgacgac gagcaaattc aaaagccgca gcgttggaat
    32221 ccggcggatc tggggcgctt tatgatcttc ttcggaccga tcagctcgat cttcgatatt
    32281 ctgacgtttt gcctgatgtg gtgggtattc catgccaaca cgccggaaac gcaaacgctg
    32341 ttccagtcgg gatggtttgt ggtgggctta ctgtcgcaaa cgctgattgt gcatatgatc
    32401 cgcacccgcc gtgtgccgtt tattcagagc tgtgcatcgt ggccgttaat gatcatgacc
    32461 gtgatcgtga tgattgtcgg gatcgcattg ccgttttcac cgctggccag ttatctgcaa
    32521 ttacaggcgc tgccgttaag ctatttcccg tggctggttg cgattctggc agggtatatg
    32581 acattaaccc agttggtgaa agggttctat agccgtcgtt acggctggca ataaagaata
    32641 aactgggcac gatagccccg attcagattg cagacaaagt aagctttgtt catgccggat
    32701 gcggcgtaaa tgccttatcc ggccaacaaa ttcaataaat tgcaggaatc acgtaggcct
    32761 gataagcgta gcgcatcagg cgattttgcg tttgtcatca gacttaatcc gggcatgata
    32821 gcccggattt ccatcaagat tagcgacgaa cagcgatcgc ttcgatctca atcttcacgt
    32881 ctttcggcag acgggcaact tcaacgcaag aacgtgccgg gaaggtggcg ttgtgttcgg
    32941 tgaagaaggc ttcgtaagtg gcgtttacgg ttgcgaagtc gttcagatct tttacaaaca
    33001 cggtagtttt aacgatgtcg cccactttca gaccagcggc ttcgacgatc gcttttacgt
    33061 tatccagcga ctgacgtgcc tgtgcagcga cgtctgccgg tacttcgccc gttttcggat
    33121 ttaccgggat ctgaccggaa gtgatgatca tattgcccag atcaacgccc tgtacgtaag
    33181 gaccgatagc tgccggtgca ttttccgtcg cgatagtttt gctcatgatt tctcctttat
    33241 tacagcggta aaaagtctgg ctcattatag ggagccagac ttttattacc aaccgcaatt
    33301 aattggccag caccacatta tgggaaaact ctttttcaca gtatttgcat ttgagcgcga
    33361 tatcattggc gcgttttcgc acggcaaagc tggaagaaac cggttcggca tggctgatac
    33421 agttgctgtt cgggcagacc agcacattgt cgatgcgctc cggcagactt gggcgcgatt
    33481 tacccaccac ttcatagttg tcgatacggt taaccgtggc ttgcggcgca tacaatgcca
    33541 gttgatctac ctggtcttca ctcaaaaagg tattttcgat tttgatcaga tctttgcggc
    33601 ccatctcgcc agaaggcagg ttcagaccga tggtgatgcg ctgatccgtt tcggtcagct
    33661 tgaacagact caacagctta aaaccgatct gggcggggat atggtcaatt accgtgccgc
    33721 gtttaatagc ttcaacctgc aatttattat cgtgtgtcat ctctatttcc ccttacagta
    33781 ccagatcgcg attcagaacc agtgccagta acgcctggcg agcgaaaatc ccgttgcctg
    33841 cctgctggaa gtaccaggcg tgtggcgttt tatcaacatc cgtcgcaatc tcatcaacac
    33901 gcggcagcgg atgaagtact ttcatattgg ctttggcgtt gtggagatcg ctggcgcgaa
    33961 gaacaaactg cgctttcacg ttggcgtact cggacgggtc cagacgctct ttttgcacgc
    34021 gggtcatgta caggatgtca acttccgcca tcacttcttc aatagagctg tgcagactcc
    34081 atgcgatccc tttttcatca agcatatcca gaatgtattg cggcattgcc agcgcgtccg
    34141 gcgcgatgaa gtaaaaacgg ttgccgtcga acttcgctaa cgcctgagtc agggagtgaa
    34201 cggtgcggcc atatttcagg tcaccaacca ttgcgacgtg gagattgtcc agacgcccct
    34261 gggtttcctg aatggtgaat aagtccagca aggtttgcgt cggatgttgg ttggagccat
    34321 caccggcatt cagtaccggt acattgccgg aaaactcggt ggccaggcgc gccgcacctt
    34381 cctgcggatg acgcatcact atcgcatcga cgtaagtgct gataaccgaa atggtatcgg
    34441 ccagcgtttc gcccttttta cccagtgatg tattggcgct gtcggagaag cccaccacgc
    34501 tggcacccag gcggtgcatg gaagtttcga aagagaggcg ggtacgggta gaggcttcga
    34561 agaaacagct ggcaatgact ttgtgcttca acagctctgg ttgcgggttt gctttcagtt
    34621 tcgccgctgt cgccagcacc agattaaggt catcgcgact aaggtcgttt atggaaatga
    34681 tatgtttctg atatagcgga ttagccatct tttatctcct gacgcctggg caaaaaaaag
    34741 cccctcgatt gaggggctgg gaatgggtga tcaacgggaa gaaaaacggc aggccagcgt
    34801 cttttttcag acgcggtaag acaaaatgtc gaacacactg aaccatacat cctcccggca
    34861 aattgtccgg cattatactc atcgtcagaa gcggtgcaag catttgatgc aatattttgt
    34921 cagcgcaaac ggtttatttg aattataagt caaaatatat gtatttttat ttactgtgat
    34981 tctggagggg aactgtcgtt cggtatcaat aattgcagac atttcctgct tttttctttt
    35041 tcactttcac gcaatcagat atgcatttta ttccattcat ccgacttata gggcggagtg
    35101 tgaaaacgaa cggctaacac tattgcttac agctcaggga tgcgcgctat cactttaatt
    35161 tcaaaatcaa agcctgccag ccatgtaacg cccaccgccg tccaggttgg ataaggtggc
    35221 gcgctaaata tttcattttt caccgtcatg atgtcttcaa attgtttttc tggatcggta
    35281 tgaaagctcg taacatcaat gatatcgtca aaagtgcatc ccgcagctgc cagggtcgca
    35341 tgcaaattat caaatgccag tctgacttgt tgctgaaaat cgggttctgg tgttccgtcc
    35401 tctcgacttc ctacttgccc ggaaacaaac agcaaatcgc cggaacgaat agccgcagaa
    35461 taacgatgtt ctgcatacag tgaatgtcgg ccagcaggga aaacagcggt tctttctacc
    35521 atttggttat cctcaagatt tacgacatga acagaagatt tatatttacc aggagccgct
    35581 tttagcggac gacgtgagta aacaaaaccc agacatcatg ggaaatggct gggcttaatt
    35641 gagcgtagtc ggttatgcgc caaacgcgcc atcaatggta tgcatcgcgc cggtaacaaa
    35701 actggcttct ggccctgcta accatgcgac cataccagcg acctcttccg gttgcccatg
    35761 tcttttgata gccataaaac tatgcaacat atcgcgcatt ggcccgttgg cgggattagc
    35821 gtcggtatca attggccctg gctggacgac gttgatggtg atcccacgcg gtccaaaatc
    35881 acgggccagc ccgcgcgcca tgccttgtag ggcagattta ctggcggcat aagcagccat
    35941 gcctgcaaca ggcatacgat cgccattcac ggagccgatg attaagatgc gcccaccttc
    36001 gggcatctgc cgggcggctt caacagaggc atggtaagga gcatgaatat tgattttgaa
    36061 aaggcgatca atatcgtcgg catttaattc cagggcatcg ccaaagacgc caatacctgc
    36121 atttaccacc aggatatcca atgcgccgct cttacgaacg acatcaatga cagcgtctct
    36181 gtcagcacta tctgtaaata ctgctgtcgc tcccgtctct tgtgccaggc gttcagcggc
    36241 atctttcgac cccgcatagg tgaatcgtac attggcccca tcggtgacga aacgacgtac
    36301 gatagcggca ccgattccac gactgccacc gaggatgaga actgtcttac ctgtaaaagc
    36361 gcccataagg actccttgat ttattatgta acatgcatta caaaactgtt ttaactttct
    36421 gtcaacaagt tttgtaatgg tcactaaaaa acaatctcgc gttccaggtc gtcccagacg
    36481 tttcgctcct gagcaggcag tctctgcggc aaaagtgctt tttcaccaaa aaggtttcga
    36541 tgctgtcagt gttgctgaag ttactgatta tcttggtatt aaccccccga gcctctacgc
    36601 ggcttttggc agtaaggctg ggttatttag ccgtgtactc aatgaatatg tcggtacgga
    36661 agctattccg cttgtcgata ttcttcgtga tgatcgtccg gtaggggagt gcctggctga
    36721 ggtattaaaa gaagcggcgc gcagatatag ccaaaacggc ggctgcgctg gctgtatggt
    36781 tcttgaaggc attcatagtc atgatccaca agcgcgtgat attgccgttc aatattatca
    36841 cgccgcagaa acggccattt atgactatat cgccaggctg catccacaaa gcgcacaatg
    36901 tgtgactgat tttatgagta ccgtgatgtc agggctttct gcgaaggcac gagaggggca
    36961 ctccctcgaa caactctgtg caactgctgc actggcgggg gaagcgataa aaactattct
    37021 caaggagtga tgctggcctt gatccgaaag gcgggaacgc gcctgccgat aagttgtgat
    37081 aagacaataa ttcacgcgtt aaggctggct gaattgatca tcttttcgta taacggtgga
    37141 aatgaaacat tgttttaatt aaggagtgga aaagatgatc atcggaaata ttcataatct
    37201 tcagccgtgg ctaccccagg agttacgcca ggcgattgag catatcaaag cacacgttac
    37261 ggcagaaacg ccaaagggca agcacgatat cgaaggtaat cgtctgtttt atcttatctc
    37321 ggaagatatg accgagccgt acgaagctcg ccgtgcggag taccatgccc gctatctcga
    37381 cattcagatt gtgttaaaag gtcaggaagg catgaccttc agcacacaac cggcaggtac
    37441 gccggatacc gactggttag ctgataaaga catcgcattt ttgccggaag gcgttgatga
    37501 gaaaacagtt atccttaatg aaggtgattt tgttgtgttt tatccggggg aagtgcataa
    37561 accgctgtgc gcagtgggtg caccagccca ggttcgcaaa gcagtagtga agatgctgat
    37621 ggcgtgatga cttttcgccg taaataactc caggtttacg gcgagtttgt gaaaagagcg
    37681 ttttttgata tttttttgtg agtaaaattt gtaatgctta gacgttctta ttaactcaag
    37741 gagttcgtca tgagcaaaat atcagatttg aattattctc aacacattac attagccgac
    37801 aattttaaac aaaaaagtga agttttaaat acctggcgtg ttggaatgaa taattttgcc
    37861 cgtaatgccg gggg
//
LOCUS       DAZKIF010000031        37527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZKIF010000031 DAZKIF010000000
VERSION     DAZKIF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.031"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            219..1592
                     /gene="aroP"
                     /locus_tag="ACHOTW_001614"
     CDS             219..1592
                     /gene="aroP"
                     /locus_tag="ACHOTW_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP9843743.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHOTW_001615"
     CDS             complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHOTW_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP9843744.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHOTW_001616"
     CDS             complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHOTW_001616"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP9843745.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3125..4018
                     /gene="nadC"
                     /locus_tag="ACHOTW_001617"
     CDS             3125..4018
                     /gene="nadC"
                     /locus_tag="ACHOTW_001617"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP9843746.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHOTW_001618"
     CDS             4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHOTW_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP9843747.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4671..6056
                     /gene="gspE"
                     /locus_tag="ACHOTW_001619"
     CDS             4671..6056
                     /gene="gspE"
                     /locus_tag="ACHOTW_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP9843748.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6046..7248
                     /gene="hofC"
                     /locus_tag="ACHOTW_001620"
     CDS             6046..7248
                     /gene="hofC"
                     /locus_tag="ACHOTW_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP9843749.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHOTW_001621"
     CDS             complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHOTW_001621"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9843750.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8482..8526)
                     /locus_tag="ACHOTW_001622"
     CDS             complement(8482..8526)
                     /locus_tag="ACHOTW_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843751.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8551..9171
                     /gene="coaE"
                     /locus_tag="ACHOTW_001623"
     CDS             8551..9171
                     /gene="coaE"
                     /locus_tag="ACHOTW_001623"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9843752.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9171..9914
                     /gene="zapD"
                     /locus_tag="ACHOTW_001624"
     CDS             9171..9914
                     /gene="zapD"
                     /locus_tag="ACHOTW_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP9843753.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9924..10121
                     /gene="yacG"
                     /locus_tag="ACHOTW_001625"
     CDS             9924..10121
                     /gene="yacG"
                     /locus_tag="ACHOTW_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP9843754.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10212..10610)
                     /gene="mutT"
                     /locus_tag="ACHOTW_001626"
     CDS             complement(10212..10610)
                     /gene="mutT"
                     /locus_tag="ACHOTW_001626"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP9843755.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(10670..13375)
                     /gene="secA"
                     /locus_tag="ACHOTW_001627"
     CDS             complement(10670..13375)
                     /gene="secA"
                     /locus_tag="ACHOTW_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9843756.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13437..13949)
                     /gene="secM"
                     /locus_tag="ACHOTW_001628"
     CDS             complement(13437..13949)
                     /gene="secM"
                     /locus_tag="ACHOTW_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP9843757.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14180..15097)
                     /gene="lpxC"
                     /locus_tag="ACHOTW_001629"
     CDS             complement(14180..15097)
                     /gene="lpxC"
                     /locus_tag="ACHOTW_001629"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP9843758.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15198..16349)
                     /gene="ftsZ"
                     /locus_tag="ACHOTW_001630"
     CDS             complement(15198..16349)
                     /gene="ftsZ"
                     /locus_tag="ACHOTW_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9843759.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16410..17672)
                     /gene="ftsA"
                     /locus_tag="ACHOTW_001631"
     CDS             complement(16410..17672)
                     /gene="ftsA"
                     /locus_tag="ACHOTW_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9843760.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17669..18499)
                     /gene="ftsQ"
                     /locus_tag="ACHOTW_001632"
     CDS             complement(17669..18499)
                     /gene="ftsQ"
                     /locus_tag="ACHOTW_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP9843761.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18501..19421)
                     /gene="ddlB"
                     /locus_tag="ACHOTW_001633"
     CDS             complement(18501..19421)
                     /gene="ddlB"
                     /locus_tag="ACHOTW_001633"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9843762.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19414..20889)
                     /gene="murC"
                     /locus_tag="ACHOTW_001634"
     CDS             complement(19414..20889)
                     /gene="murC"
                     /locus_tag="ACHOTW_001634"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9843763.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20943..22010)
                     /gene="murG"
                     /locus_tag="ACHOTW_001635"
     CDS             complement(20943..22010)
                     /gene="murG"
                     /locus_tag="ACHOTW_001635"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9843764.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22007..23251)
                     /gene="ftsW"
                     /locus_tag="ACHOTW_001636"
     CDS             complement(22007..23251)
                     /gene="ftsW"
                     /locus_tag="ACHOTW_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP9843765.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23251..24567)
                     /gene="murD"
                     /locus_tag="ACHOTW_001637"
     CDS             complement(23251..24567)
                     /gene="murD"
                     /locus_tag="ACHOTW_001637"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9843766.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24570..25652)
                     /gene="mraY"
                     /locus_tag="ACHOTW_001638"
     CDS             complement(24570..25652)
                     /gene="mraY"
                     /locus_tag="ACHOTW_001638"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9843767.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25646..27004)
                     /gene="murF"
                     /locus_tag="ACHOTW_001639"
     CDS             complement(25646..27004)
                     /gene="murF"
                     /locus_tag="ACHOTW_001639"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9843768.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27001..28488)
                     /gene="murE"
                     /locus_tag="ACHOTW_001640"
     CDS             complement(27001..28488)
                     /gene="murE"
                     /locus_tag="ACHOTW_001640"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP9843769.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28475..30241)
                     /gene="ftsI"
                     /locus_tag="ACHOTW_001641"
     CDS             complement(28475..30241)
                     /gene="ftsI"
                     /locus_tag="ACHOTW_001641"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP9843770.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30257..30622)
                     /gene="ftsL"
                     /locus_tag="ACHOTW_001642"
     CDS             complement(30257..30622)
                     /gene="ftsL"
                     /locus_tag="ACHOTW_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9843771.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30619..31560)
                     /gene="rsmH"
                     /locus_tag="ACHOTW_001643"
     CDS             complement(30619..31560)
                     /gene="rsmH"
                     /locus_tag="ACHOTW_001643"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9843772.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31562..32020)
                     /gene="mraZ"
                     /locus_tag="ACHOTW_001644"
     CDS             complement(31562..32020)
                     /gene="mraZ"
                     /locus_tag="ACHOTW_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9843773.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32622..33626)
                     /gene="cra"
                     /locus_tag="ACHOTW_001645"
     CDS             complement(32622..33626)
                     /gene="cra"
                     /locus_tag="ACHOTW_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP9843774.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33708..33794)
                     /locus_tag="ACHOTW_001646"
     CDS             complement(33708..33794)
                     /locus_tag="ACHOTW_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP9843775.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33806..34297)
                     /gene="ilvN"
                     /locus_tag="ACHOTW_001647"
     CDS             complement(33806..34297)
                     /gene="ilvN"
                     /locus_tag="ACHOTW_001647"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9843776.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34300..36024)
                     /gene="ilvI"
                     /locus_tag="ACHOTW_001648"
     CDS             complement(34300..36024)
                     /gene="ilvI"
                     /locus_tag="ACHOTW_001648"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP9843777.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36342..37286)
                     /gene="leuO"
                     /locus_tag="ACHOTW_001649"
     CDS             complement(36342..37286)
                     /gene="leuO"
                     /locus_tag="ACHOTW_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP9843778.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9085 a        10451 c         9140 g         8851 t
ORIGIN      
        1 ctgagcttta caaacggttt ctttttaagc aactcatctt caaccatgca taaagcgggt
       61 gcattcgctg ccgcatacca ttattcttga tctgacggaa gtctttttgt aactattcaa
      121 acttctttga tgtaaacaaa ttaatacaac aaacggaatt gcaaacttac acacgcatca
      181 ctgcgtagat caaaaaaaca atcaccgcac gaggtttcat gatggaaggt caacagcacg
      241 gcgagcagct aaagcgcggc cttaaaaacc gccatattca gcttatcgcg ctgggtggcg
      301 cgatagggac agggttattc ctgggtagcg cctccgtaat acagtccgca gggccaggga
      361 ttatcctggg ttacgccatt gctggtttta tcgcctttct gatcatgcgt cagctgggtg
      421 aaatggtggt cgaagaacct gtcgcaggct cctttagcca ctttgcttat aaatactggg
      481 gcagctttgc tggcttcgct tctggctgga actactgggt actgtacgtt ttagttgcca
      541 tggcagagct gactgctgtg ggtaaataca ttcagttctg gtatccggaa atcccaacct
      601 gggtttctgc cgccgtgttc tttgtggtga ttaacgccat caacctgacc aacgtaacag
      661 tgtttggtga gatggagttc tggtttgcca ttatcaaagt tattgcggta gtagcgatga
      721 tcatcttcgg cggctggctg ctgttcagtg gtaacggcgg tccgcaggca agcgttagca
      781 acctgtggga tcagggcggt ttcctgccgc acggcttcac cgggctggtg atgatgatgg
      841 cgattatcat gttctcgttc ggtggtctgg aactggtggg gatcaccgca gcagaagctg
      901 ataacccgga gcaaagtatc ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt
      961 tctatattgg ttcgttagcc gttctgctct cactgatgcc gtggacccgc gttaccgccg
     1021 ataccagtcc gtttgtgctg atcttccacg agttaggcga tacctttgtg gcgaatgcgc
     1081 tgaacatcgt ggtactgact gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca
     1141 gccgtatgct gtttggtctg gcacaacagg gtaacgcgcc aaaagcgctg gcgtctgtcg
     1201 ataaacgcgg cgtaccggtt aacaccattc tggtgtctgc gctggttaca gcattgtgcg
     1261 tattgattaa ctatcttgct ccggaatccg catttggcct gttaatggca ctggtggtat
     1321 ccgcactggt gatcaactgg gcgatgatca gtctggcgca tatgaagttc cgtcgcgcca
     1381 agcaggaaca aggcgtggta actcgcttcc ctgctctgct ttatccgctg ggtaactgga
     1441 tctgcctgct gtttatggcg gtggtactgg tgattatgct gatgacccca ggaatggcga
     1501 tttcggtata cctgatcccg gtatggctgg tggtgttagg tatcggctat ctgtttaaag
     1561 agaaaaccgc aaaagccgta aaagcacatt aatctctcta cgccctcacc cgtacagggt
     1621 gagggcaata atctttacac caacgccccg taaatcgtca gtagcgcaat caccaccacg
     1681 accacgaacg aggttttctt cgccattgaa accgctgcct tcggcgtctc caccttatcg
     1741 acatgcggtt cacgcgccag agagaactgc gccagacgcg ttaatacctg atattgcgaa
     1801 gtacggaaat cacccagcga agcaaaccag gccggtaacg ctttctcacc atgaccgatc
     1861 aaggcatata ccacacctgc aagacgaacc ggcacccaat ccagtacatg aagcactgca
     1921 tcaatgccgg actgtaaacg atgatgcggc gtctggtatc gcgccagcca gtattgccat
     1981 gcacgcaaaa acgcataccc catcagcgta acgggtcccc aggttccccc cacaatcagc
     2041 cagaacagcg gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca
     2101 cgcaaatact cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt gagttcgcca
     2161 gccatcgtgg cacgggcatg gctatcatta cgtgaagcag ctgtcagata agcatgataa
     2221 tgaagacgaa ctttacctgc gccaatacac agcaaaccaa tcagcagcca caccagcagc
     2281 gtgggaacgt tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc
     2341 gcaataatgg tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac
     2401 gcttcaagac gatgatcaag ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt
     2461 aacaccagta aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc gcacgaaacc
     2521 gtgcccaatc aaatgcagga ccgggatcgg ttttccgatc cggcgcaata tcacaatggc
     2581 ccgtcatgtt attagcgata tccggatagc aatcaatcag tgcccgcgta accgccgcaa
     2641 gctgttgata ctgcgcatcg gtatacgcca gcgtatcggt gccttcaagc tcaatcccaa
     2701 tagaaaaatc attgcagcgt tcgcgcccct gatactgaga gactcccgca tgccatgcac
     2761 gtttatcgaa aggaacatac tggactattt caccatcacg gcgaatcaaa cagtgagcgg
     2821 agacgcgcaa atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca atagttccag
     2881 tgaataatgc gtcgatccac ggaccgccaa actcgcctgg cggcaggcta atattgtgca
     2941 ccaccagcag ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc
     3001 ggcgcgcgcc aaccagccac ccctgttcta acaacatgca ggatctcctt atatgtggtg
     3061 ctaatacccg gttcagagta gcatgtttct accttatgat tcgttagcta tctggagttt
     3121 taacatgccg cctcgccgct ataaccctga cacccgacgt gacgagctgc tggaacgcat
     3181 taatctcgat atccccggcg cggtggccca ggcgctgcgg gaagatttag gcggaacagt
     3241 cgatgccaac aatgacatta cggcaaaact tttaccggaa aattctcgct ctcatgccac
     3301 ggtgatcacc cgcgagaatg gcgtattttg cggcaaacgc tgggttgaag aggtgtttat
     3361 tcaactggca ggcgacgatg tcaccataat ctggcatgtg gatgacggcg atgtcatcaa
     3421 cgccaatcaa cccttgttcg aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg
     3481 cactgcgctt aattttgtgc aaaccctttc aggagttgcc agtaaggtac gccactatgt
     3541 cgaattactg gaaggcacca acacgcagtt gttggatacg cgtaaaacct tacccggtct
     3601 gcgttcagcg ctgaaatacg cggtactttg cggcggcgga gcgaatcacc gtctggggct
     3661 ttctgatgcc ttcctgatca aagaaaacca tattattgcc tccggctcag tgcgccaggc
     3721 ggtcgaaaaa gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag tagagaatct
     3781 ggaagaactt gatgaagccc tgaaagcagg agccgatatc atcatgctgg ataacttcga
     3841 aacagaacag atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc
     3901 tggcaacgtc actgacaaaa cactgcgcga atttgccgaa acgggcgtgg actttatctc
     3961 cgtcggtgcg ctaactaagc acgtacaggc actcgacctt tcaatgcgtt ttcgctagtt
     4021 gctgacagat ttatccgctc catagtgggc ggataatctc cccttcccca accttcttcg
     4081 taacgcctcg caaatttctt gtctttcgct gcaacgaaat aaaaaacctc ggaagcagct
     4141 ttcccgtttc gttttttgcc cctcgcctgt gctgatggcg tttgccaaag tagcaccaac
     4201 caaatcaagg agcgaaacag atggacaagc aacgcggttt tacacttatc gaactgatgg
     4261 tggttattgg catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc
     4321 tgcgcaaagc cgcactcacc gacatgctac aaacctttgt gccttaccgt accgccgtag
     4381 agttgtgcgc gctggaacat ggtggattag atacctgcga cggtggcagc aatggcattc
     4441 cctcgcctac caccacccgc tatgtttcag ccatgagtgt ggcaaagggc gtggtgtcgc
     4501 tgaccgggca agaaagtctc aatgggctaa gcgtcgtcat gacgccaggt tgggataacg
     4561 caaacggcgt caccggctgg gcgcgcaact gcaatattca aagtgacagc gcattgcagc
     4621 aagcctgcga agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc
     4681 cacagctcac ggccctgtgc ctgcgttatc agggagtctt gctggatgcc agcgaagaag
     4741 tggttcatgt tgcggtggtc gatgccccct cacatgagtt gctggacgca ttgcatttcg
     4801 ctaccaccaa acgtattgag atcacctgct ggacgcgcca acaaatggaa ggtcacgcca
     4861 gtcgcacaca acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt
     4921 tgctgactcg aacgttacaa tctgcgctgg aacaacgcgc gtctgatatt catatcgaac
     4981 cagcggacaa tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg
     5041 atgtttcacc ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg
     5101 atattgcgga acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg
     5161 ccgtctcatt tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg gtattaaggt
     5221 tgttacagca ggtgagccag gcactggatg ttaacacgct gggaatgcag ccgttacaac
     5281 tggcgggctt tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta
     5341 ccggcagcgg caaaacggtc acgctttata gtgccctgca aaagctgaat accgctgaca
     5401 ttaatatttg tagcgtcgaa gatccggttg agatccccat agccggacta aaccagacgc
     5461 aaatccatcc gcgtgccgga ctcacctttc agggcgtttt gcgtgcgtta ttgcgccagg
     5521 atcctgacgt catcatgatc ggagagatcc gcgatggcga aacggcagaa attgccatta
     5581 aagccgcgca aaccggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa
     5641 cgctggtacg tttacagcaa atgggggtcg cccgctggat gctatcatcg gcgcttacgc
     5701 tggtaatagc ccagcgtctg gtacgcaaac tttgcccaca ttgtcgccgg cagcaagggg
     5761 agcccatcca cattccagtc aatgtatggc cgtcgccgct gccccactgg caagcacccg
     5821 gttgtgtaca ttgctaccac ggtttttatg gtcgcactgc cttatttgaa gttctgccca
     5881 taacaccggt catacgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc
     5941 acgcccgaca ggcgggtatg cgaacgcttt ttgaaaacgg ctgcctggcc gtggagcaag
     6001 gcttaaccac ctttgaagag ttaatccgcg tattggggat gccgcatggc gagtaagcaa
     6061 ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc aagatgggat gctatgggca
     6121 gagagccgta ctttactgct tatggcacta cagcaacaga tggttacccc actaagcctg
     6181 aagcgaattg ctatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat
     6241 caactggcga cgctacttaa agccgggtta acgctttctg aagggctggc actgctggcg
     6301 gaacagcatc ccagtaagca atggcaagcg ttgctgcaat ctctggcaca cgatctcgaa
     6361 cagggcattg ccttttccaa tgccttatta ccctggtcag aggtatttcc gccgctctat
     6421 caggcgatga tccgcacggg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg
     6481 gcgcgtcagc aaagagccca gcgtcagttg accgacaaag taaaatcagc gttacgttat
     6541 cccatcatca ttttagcgat ggcaatcatg gtggttgtgg caatgctgca ttttgttctg
     6601 ccggagtttg ccgctatcta taagaccttc aacaccccac taccggcact aacgcagggg
     6661 atcatgacgc tggcagactt tagtggcgaa tggggctggc tgctggtgtt gttcggcatt
     6721 atgctgacga tagccaataa gttgctgatg cgccgcccga cctggcttat cgcgcggcag
     6781 aaattgctgt tacgcatccc gattatgggg tcactgatgc ggggacaaaa actcacgcag
     6841 atctttacga ttctggcgct gactcaaagt gcaggcatta cttttttaca gggcgtagag
     6901 agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca aatccagcac
     6961 gatatcagta acggtcatcc catctggctg gcgttaaaaa atgccggtga gtttagcccg
     7021 ctctgtttgc aattagtgag aacgggagag gcctccggct cgctggatct catgttagac
     7081 aacatcgccc atcatcatcg ggataacaca atggcgctgg cggataacct agcagcctta
     7141 ctggaaccgg cgttgctgat cataacggga ggaattatcg gtacgctggt ggtggcgatg
     7201 tatctgccaa ttttccattt aggcgatgcg atgagtggga tgggataacg ctggcgtgtt
     7261 gctccacacc agcgttggaa gattacaggt tgttgaagat gcggttttct tgttcctgca
     7321 cacggataaa tgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca acgtatgtac
     7381 aagctgaacg caggccgccc aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg
     7441 gcagcttaac ggttttacct tctgctgcgc gatattcagc aacgccgcca acgtgacgtt
     7501 tcatcgcaga ctcggagctc atgccgtaga acagcataaa tttctcgccg ttctcctcaa
     7561 cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg
     7621 caccgccacc aaaggctttc gccacatcgc ccggcgtggt gcagccacca tcgctgatga
     7681 tcattccgcc cagaccgtgc gcagcatcgg cacattcgat taccgcagaa agttgcggat
     7741 aaccgacgcc tgttttgacg cgagttgtac aaacagaacc tgggccaatg ccaactttaa
     7801 cgatatcggc acctgagagg ataagctcct cacacatttc accagtcact acgttaccag
     7861 cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt
     7921 cggaataacc attcgccacg tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa
     7981 tctgtttagt tttttcgaaa tccgcatcag acgtaccggt agaaaccatc acatgtttca
     8041 gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa tagtgtttat
     8101 gcacagcagt caaaatatca aaagaagcca gcgcagaggc catagaaaat gtgcctacgg
     8161 tgtccatatt tgcggcgata atcggcacgc cggaccagct ctgacctgaa tgtttgaagg
     8221 tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt ttagggcgga
     8281 tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc ggattcctgg
     8341 ggttaatggc tataagggta agagcgcaac tccagtgacg ctatcatacg cactaatcaa
     8401 tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa tttacctggt
     8461 aaatagtgag cgaacgtttc atcaatttcg ctttcgtgcg ctcattttta acgttttgat
     8521 aatcatgatg aattcccggg aatataaatt atgaggtata tagttgcctt aacgggaggc
     8581 attggcagtg gcaagagtac cgttgccaat gcgtttgctg atctcggaat taacgtcatt
     8641 gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct acatgccatt
     8701 gctgatcact ttggcgctaa tatgattgct gctgatggaa cattgcagcg ccgggccttg
     8761 cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgccct gctgcatccg
     8821 ctgattcagc aagagacgca acaccagatc cagcaagcta cttcccccta tgtactgtgg
     8881 gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt gctggtggtg
     8941 gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgatgatgt aactcgcgag
     9001 catgtcgaac aaatccttgc tgctcaggca acgcgcgaag cccgccttgc cgtggcagat
     9061 gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc ccgcctgcac
     9121 gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc
     9181 aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt
     9241 tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg ctgcatttct
     9301 tccgtaatgt tagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt
     9361 tgaaagaact tgaccggcag caacgtaaac tccagacctg gattggcgtg cctggcgtgg
     9421 accagagccg tattgaagca ttaattcagc agttaaaagc ggcggggagc gtattaattt
     9481 ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc
     9541 gactgagcat cccaggcggc tgttgcagct ttgatttacc tacattgcac atttggctgc
     9601 atctacccca ggcgcagcgc gactgccagg tagaaacctg gattgccagc ctgaacccgc
     9661 tcacccaggc acttaccatg gtgctggatt taattcgtca gtcggccccc ttccgtaaac
     9721 aaaccagcct gaatggtttt tatcaggata acggtggcga tgccgacttg ctgcgcctga
     9781 atctgtcgct cgattcacag ctttatccgc aaatttccgg acataagagc cgttttgcca
     9841 ttcgttttat gccgctggac actgaaaacg gacaggtacc ggaacgtctg gatttcgaac
     9901 tggcctgttg ctaaggagta aaaatgtcag aaactattac ggtgaattgc ccaacctgcg
     9961 ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc
    10021 agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc agtggcgatc
    10081 tttccgaaag cgatgactgg agcgaagagc caaagcagtg acatttgtct gatgccgcac
    10141 gtaggcctga taagacgcgg acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg
    10201 gcgtgaacgc cttatccgac atacacacgc ttaagcttcg caattaccgg ttcattggct
    10261 ggcggaaaat catcggcatt aagaccgact agcgacatcc acttaccggg ttgcccttct
    10321 ttaccccacg gctccccttc ccagctttcg accagccaga accacagagt tatatgcctg
    10381 tccgggaatt catattccag tttttcaaat agcgaaaaat gttgtggggt aatcccgact
    10441 tcttcctgaa gttcacgcac caccgcctgt tccggcgttt cacccatttc aattttaccg
    10501 ccgggaaact ccagtttatt cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt
    10561 tcattgttct cgttgcgaat aatacctacc gcaatttgca gctttttcat tgtcttaaac
    10621 ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac agttagcttt tattgcaggc
    10681 ggccatggca ctgcttgtat tttttaccag aaccgcacgg gcaaggatcg ttacgtccta
    10741 ctttgcgctc accggtttgc gccgccagtg cagctgcggc tgcagagtcg tcatcctgat
    10801 ggctaagctg ctgcatttgc gctaaacgct cggcttccat acgacgctgt tgttccagct
    10861 cctcaacctc ttcaggcata cgtacctgaa ctttgctcag cgtactgata acttcatatt
    10921 tcaacgactc cagcatcgct gcaaacatgg agaacgattc acgtttgtat tcctgcttcg
    10981 gatctttctg tgcatagcca cgcaggtgga taccctgacg cagatagtcc atcgctgcca
    11041 ggtgctcttt ccacagggag tcaagcgttt gcagcatgac gcctttctcg aagtgacgca
    11101 tcatctcagc accaaccact tcttctttac gctgatacac ttcgatggac tgcgccagaa
    11161 tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt atccagccac tcagcaattg
    11221 gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag ccccggaata tcccacattt
    11281 cttccagcga ctgtggcgga atgtaggcat caatggtcgc tttgaacaca tcttcacgaa
    11341 tgctgttaat ggtttcgctc acatcgctga catccagcaa ttcgttacgc tgggagtaaa
    11401 tggcgcgacg ctgatcgtta gccacgtcat catattccag cagttgctta cgaatgtcga
    11461 agttacgact ttcaacttta cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt
    11521 caatggcttc gcctggcttc atacccagtt tacgcatcat gccggatact cggtcggaag
    11581 caaaaatacg catcagcgca tcttccatcg acaggtagaa acgggaagaa ccagcatccc
    11641 cctgacgacc agaacgaccg cgcaactggt tatcgatacg acgggattcg tgacgctcgg
    11701 taccgatgat atgcaggcca cctgctgcca gtaccgcatc gtgacgtacc tgccagtcgg
    11761 ctttaatttt ttcaatttgc tctgcggtcg gattttccag cgcggcaact tctgcctgcc
    11821 agctaccacc gagcacaata tctgtaccac gacccgccat gttggtcgcg atagtcaccg
    11881 cagccggata acctgcctga gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca
    11941 ggacattgtg cttaataccg gctttggtca gttcgtttga caccagctcc gatttttcga
    12001 tggagatagt acccaccagc accggctggc ctttcgcagt acgttctttg atatcttcaa
    12061 tgatcgcctg aattttttcc gcttcagtca tgtagaccag gtccggcaga tctttacgaa
    12121 tcattggacg gttggtcgga acaacgacgg tatccagttt gtagatggag ctaaattcga
    12181 aagcttcggt atcagcagta ccggtcatcc ccgccagttt ttcatacaga cggaagtagt
    12241 tctggaaggt gatcgaagcc agcgtttggt tttcgttctg gatctgcaca ccttctttcg
    12301 cttccacagc ctggtgcaga ccatcggacc agcgacggcc ctgcatggta cgaccggtgt
    12361 gttcgtcaac gatgataact tcaccatctt taacgatgta gtcgacgtca cgggtaaaca
    12421 gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag catgatgttg gccggagagt
    12481 acagagactc cccttcatcc atgatgccct ctttcaccag cagttcttca atcagcacca
    12541 gaccacgttc ggtcaggttc acctggcgag atttttcgtc caccgagaag tggccttcgc
    12601 cctggaaggt ttcggagtct tctttttcct gacggatcag gtgcggaata attttattca
    12661 cgcgtttata catttccgag ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg
    12721 cttcatcgat caggatggag tccacttcgt ccaccagcgc atagtgcagt ttacgctgta
    12781 cacgttcttc agggctgaac gccatattgt cgcgcaggta gtcaaagccg tattcgttgt
    12841 tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg
    12901 gcaggttgat accgacagtc aggccaagga attcaaacag cggacggttg ttttcggcgt
    12961 cacgttgcgc caggtagtcg ttgacggtaa ctacatgcac gcctttaccg gttagtgcgt
    13021 tcagataagc aggcagcgtt gcggtcaggg ttttaccttc accggtacgc atttcggcga
    13081 tgcagcgttc gttaagaacc ataccgccga gtaactgaac gtcgaagtga cgcataccaa
    13141 agacgcgctt acttgcctca cgtaccacgg cgaaagcttc cgggatcaga ttttccagca
    13201 cttcgccttt ttccagacgt gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg
    13261 agagtttttc catctccggt tccatggcat tgatgatgtt gaccactttg cgcatccggc
    13321 gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa taatttgatt agcataataa
    13381 aatctcaaac gccccgcgtt gcggagttaa taaaatgaag taaaggttta ttgttgttag
    13441 ctgaggcgtt gagggccagc acggatgcct tgcgcctggc ttatccagac gggcgtgctg
    13501 aattttgctt gtggggtaaa atgcgcataa tcaatgcgat aacccttttc agacggcgtg
    13561 ccttcctggg tcagcagcgc gctgagcgta tccagtaatg caagatgttg cgcctgaaga
    13621 ggcaaagatt cttcagcaac gggcagcgtt tgcggtgcca ttgcgaagga aagatgacgg
    13681 attaccgtgc gaatggcatg ttgatgccag taatctacgg aatagttcga attcgggcgg
    13741 cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa ctttggctga aggctcgtgg
    13801 ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa
    13861 cctaaactcg ccgcaaccat ccctaataag agatgcggcc agaagtagcg tttaccaaac
    13921 tgtcgccagc gcgtcagtat tccactcacg ttattgccat cccgatcgta ttcaaacgta
    13981 tttagcgcac aaatcttatc attaaagcct gaagtccaca gcacgaatga agataagttg
    14041 tccgtaaaag tgcttaagaa aacagcgttc gcaccgtttt ttatagctct tataaaattc
    14101 aaaaaaaaac gactggttca cctggccgga gagagtgcca gatttgccag tcgaatttta
    14161 tacgacagta taaatgtcgt tatgccagta cggctgaagg cgctttgaag gccaacggca
    14221 gttctgcgtc gtcctggaag gtcacatatt cccaggcttc ctgtttcgcc aggacagcct
    14281 gcagcagttt gttattcagt gcatgaccgg atttataagc ggtaaatgca ccaataatat
    14341 tgtgaccaca catgaacaag tcaccgatcg catcgagcat tttgtgacgc acaaattcgt
    14401 cttcaaaacg caggccgtct tcgttcagta cgcgataatc gtcaacaacg atggcacaat
    14461 cgaagctgcc gcccaggcac aaaccacggg actgcagata ttcgatatca cgcatgaaac
    14521 cgaacgtacg cgcacggctg atctggcgca taaacgcatc agcggagaag ttcatcgcat
    14581 agcgctggtt gctggaatca atagccggat ggttaaaatc gatggtgaaa tccagcgaaa
    14641 aaccattgta cggcttaaat tcagcccact tatcgccatc ttcgacacga acagtctctt
    14701 tgatgcgaac aaattttttg gcgcagttca actcgtcgat accggcgtca agcagcaggt
    14761 atacaaacgg agcggcgctg ccgtccatga tcgggatttc cggcgcgtta acttcgataa
    14821 caatgttatc gatgcccaag cccgcgagag cagcattgag gtgctctacg gttgaaatcc
    14881 gtacatcatg ctcgttgacc agacacgtac agagcatggt atcacgcaca gatttggcat
    14941 cggccgggaa atctaccggt ggattcaagt cggtgcgacg atagatgacc ccggtgttgg
    15001 ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt atgtaaaccg acacccgtcg
    15061 cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt attatctcgc caaattacct
    15121 atccaaccga agtgtactat acattcggcg ggccagttta gcacaaagag cctcgaaacc
    15181 caaattccag tcaattctta atcagcttgc ttacgcagga atgctgggat atccagataa
    15241 tccggctctt tcgcagtttg cggcgcattg tcattcacga ctttagcaac cggcttctgc
    15301 tcctgggtca gcggagccat cccatgctgc tggtagcgat ccatcactgg ctgctgaacc
    15361 tgcttattgg tcaccagagt gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca
    15421 acggttacgc gcagctcgtc attcatatcc gggtcaagag aagtaccgat aaccacagtc
    15481 gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg tttcgaactc atccagacgc
    15541 aggtcgaagc ccgctgtgat gttaaccagc acgccgcgtg cgccagacag gtcgatatct
    15601 tccagcagcg gagaagagat agccatttca gcagcttctt ccgcacggtc ttcaccgctc
    15661 gccacgccag aacccatcat tgcgtagccc atttcagaca ttacggtgcg tacgtctgca
    15721 aagtccacgt tcatcaaacc cggacgagta atcagttcgg cgatgccttg cacagcgcct
    15781 ttgagtacat cgttcgctgc gccaaacgca tccagcaggg agataccgcg gcccagaact
    15841 ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca catgcttgga cagttcagtg
    15901 atcccctgct ccgcgaatgc catacgcttc ttgccttcaa agttgaaggg cttagtgacg
    15961 acagcaacgg tcaggatacc caaatctttt gccacttcag cgacgactgg tgctgcacct
    16021 gtaccggtac caccacccat acccgcagca ataaagacca tgtctgcacc ttccagcgcc
    16081 gcacgcagtg catcgcgatc ctcatctgcc gcattgcggc caacttctgg attagcgcca
    16141 gcgcccagtc ctttggtgat accgctaccg atttgaatcg tctgtccaac cgctgtttta
    16201 cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa caccttcaat gcgctcgcgc
    16261 accatgtgtt caacagcatt accgccgccg ccgccgacgc cgatgacttt aatcaccgcg
    16321 tcattggtaa gttccattgg ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga
    16381 ggccgtaatc atcgtcggcc tcataaaaat taaaactctt ttcgcagcca actattgagt
    16441 cgcttgatcc acgagccaac tgatgctgta acacgttttt ctacttcagc ttcaccgtta
    16501 agatgtgact ctttcccata gtgaagcaat cccaccgccg tcgaataata cggctcctga
    16561 gcataatccg ttaaaccggt aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac
    16621 acgcgctgag cacaggctgc aagaccttcg atctgcgctg cgccaccggt taatacaatg
    16681 cctgccgcca ggtgatgttt aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc
    16741 tcttcgttga ccaggttgag cagctcggta tagcgcggct cgatcacctc tgccagggtc
    16801 tgacgttgca gactccgcgg cggacgaccg cctacgctcg gcacttccac gctctcatct
    16861 tttccaacga tggaacccag cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt
    16921 ggcggcgtgc caaaggcgta agcgatatca ctggtcacga cattgccagc ataaggaatt
    16981 accttagtgt ggcgcaatgc cccaccggta taaacggcga tatccattgt accaccaccg
    17041 atatcgacga cgcagacacc cagttcacgt tcatcttccg tcaataccga ataacttgat
    17101 gccagaccgg caaatatcag ttggtcaact ttcagcccac aacgttcaac cgctttgacg
    17161 atgtttttcg ccatatcgtt gtggcatgtg atcaggtgca cttttgcctg catccgcacg
    17221 cccgaaagcc ctaccggatt cttgatccct tcctgatagt caatcgcata ctcttgcggg
    17281 atcacatgca gcacacgatg ctcatcgcgt acacgcaccg atttcgcggt atggacgacg
    17341 ttttccacat cttcttgcgt tacttcttct tcagaaatag gcaccatacc aatttcattc
    17401 tggcagctga tgtgcttacc agaaagcgcc agatataccg aagagatctg acaatctgcc
    17461 atcaattctg cctggtcaat ggcacgttgt acgcacttga ccacggattc gaggtcgttc
    17521 accccgcctt tatccatacc acgcgacggg cagctgccca cgccaatgat attgaccata
    17581 ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg cggtaccaat ctccagtcct
    17641 actaccagtt ttctgtccgt cgccttgatc attgttgttc tgcctgtgcc tgattttgtt
    17701 gctgagtaga ttcctctggc ggcaagggcg cccagcctac tgccgctcca gagtcataac
    17761 gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc ctgctgctgt agaaccggat
    17821 aaagttctac aaagcgagcc aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa
    17881 tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt catcgccgct tccttcagag
    17941 taaatctgtc ctttgccagc atctgcccca tttcgcgata gccctgcaac acttcattgg
    18001 cgctgccttc cgggccatac agcattggaa gcacctgctt gctggtgcga tctggcggca
    18061 cgctgaaggt atttccttcc gcgtctacca tgtgttgatc attccaccgc gcaatcggca
    18121 catattcaac cagatgaatc ttcaattcat caggccactg ctttctgacg ctcacctgct
    18181 taatccacgg cagacgttgt tctatttgcg tctggatgat gttgacatcc tgggtcataa
    18241 aggtacccgg ctcacccaat gccaggatcg actgccggat atcgtcatta cgcgtgtaat
    18301 ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg ttgcgcatct tccatccagc
    18361 ccaacacgac ccagccgctc accaacactg tcgttaaaac ggtcagcagg aaaaggatcc
    18421 ccgccagacg cgttccatta ttgcggcgag aagaaacctc ttcttcgctg tttcgcgtgt
    18481 tcagagcagc ctgcgacata ttagtccgcc agttccagaa ttcgtactac caactgcgag
    18541 aagctcatac ctgcctgacg tgccgccatc ggcaccaggc tgtggctggt catacccggt
    18601 gaggtattgg cttccagcag ataaaactgt ccatcgctgt ccagcataac gtcaatacgt
    18661 ccccatcctt tgcaacctaa cgtcgtccat gctttcagca ctaatgcctg caaattggcc
    18721 tcttgtgacg cttccagacc tgcggggcag aaatactgtg tctcatcaga gagatacttc
    18781 gcctcataat catagaaggt tccggacggt tgaatacgta ctgacggtaa aatttcttca
    18841 ccgagtatcg caaccgtgaa ctccggccca cttagccatt tttcaatcaa tacttcttca
    18901 tcgtgctgaa atgccaatct taatgcatct tgtagagcat tttctgctac tacttttgac
    18961 attcccacac tggaaccttc gcggctcggc ttaacgataa ccggcaaacc cagagcagaa
    19021 atttctgcta actgcttatc actcaggcct ttttcaaact ctacgcgggt taacgctacc
    19081 cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt tgctgcgtag tttatccatt
    19141 gaaagcgcag atgccatcac tccgctgccg gtataaggca agcccatcag ctcgagcatc
    19201 ccctgcagcg taccatcttc accgccgcgg ccgtgtagcg cgataaacac tttctgaaag
    19261 cccatcgact tcagttgcgt cacgtcaact tctttcgggt cgacaggata tgcgtcaata
    19321 ccgccttcac gcagcccggc taacaccgct gcgccagaat tcagagaaac ttcccgctca
    19381 gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca tgttgttctt cctccggagt
    19441 ttgcggcttc agtttgattt cagctaaaga acgggcaatt tttccgatat taccagcccc
    19501 ctgaacgaga atcaggtcgt taccggttaa taccggtgcc agcatctcgg ctacctgcgc
    19561 agggtccggc accagaatgg gatctatttt cccacgtcca cgaattgtgc gacacagcga
    19621 acggctgtcc gcacccggaa ttggcgcttc gccagccgga tacacttcca gcatcaacag
    19681 ggtatcaacc tgcgtcagca cattggcgaa atcatcatac aggtcgcgtg tacgggtaaa
    19741 acggtgcggc tgaaacagca ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt
    19801 aatggtggcg tccacttccg tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc
    19861 gcttttacca ttcactggct ccagcgggaa ttcaccgagg aaatcaaaac ggcgaccagt
    19921 cccctggaag ctctccagcg cacgcaaaat agcctcgtcg tcaatgccct cttccgtagc
    19981 aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg cctggcgcat tcagggtgac
    20041 gcgcatcggc tctttgtcct ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg
    20101 ataatcttct acacgcacgt cggcatcttc gctgaagccg taagttgtgg tctgacgtcc
    20161 cactcgcggt aacaattcgc ggatcaccgg atcatcaaca cacatcaccg cacgaccgta
    20221 aaacggcagg ttgtgcagaa aattaataaa agtctgtttt aaattctcaa agtcgccctg
    20281 gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc gccaccatcg gttgcagatg
    20341 caggaacgat gcatcactct catctgcttc ggcaatcagg taccgaccat gccccaaacg
    20401 cgcatgaacc cccgccgctt ttaccagccc gccgttaacg aaggttgggt cgagccccgc
    20461 ttctgcgtag atgctggaga ccatcgcggt agttgtcgtt ttgccgtgcg ttcctgcaat
    20521 ggcgatgcca tgacgaaaac gcattaactc agccagcatt tcggcacgac ggatcaccgg
    20581 aatacgcgct tcatgagctg cgacaatttc cgggttatcg gcagaaatgg cgctggaaac
    20641 aacgaccacg ctggcatcac gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc
    20701 cagattcatt aactgctgcg tgaccggatt tggcgctaaa tcggaaccac tgatctgata
    20761 accttcattg gccagaactt cggcaatacc gcccataccg gcaccaccga tgccgacaaa
    20821 atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa cgcagttttg ccaattgttg
    20881 tgtattcatt ctttacgcca ttaacttctt aaattcatac gatgcaaaag gcatcgctac
    20941 aattacgccc gggcagcccg actcacttca tttgccactc gctcggtggc atccggaatg
    21001 gatgcagcgc gggcgcgttc tgccatggtt aataaggttt ctcgcgacca cccggccagg
    21061 gtgttggcga cagcatccac gctaagctgt ggctgctcga taattttggc tgcgcccgct
    21121 ttttccagcg gtagcgcatt ccagtattgc tggcggtctt tatgttgaaa cggcacaaac
    21181 aacgccggta gtcctgccgc ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg
    21241 acgacatccg cccacgcata cgccgccgcc atatcatcaa taaattcagt cactttatgc
    21301 tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt gttgcgaacc tttgccgctc
    21361 tgatgccaga tagtgactga atcacccagt ttcgcagcaa cctgcggcat tgtctggtta
    21421 agaatgcgtg cgccctggga accacccact accagcacac gaaccggacc ttcacgtcca
    21481 gccaaacgtt gctgcggcaa cggtagcgcc aacacatcgg tacgcaccgg gttacccacc
    21541 acttccgcat tggggaaagc acctggaaac gcctgcatca ctttggtggc aatcttcgcc
    21601 agccatttat tggttaagcc cgcaataccg ttttgttcat gaagtacaac cggaatgcct
    21661 aacgaccacg cggccagacc accaggacct gatacgtagc cgcccatacc gagcaccacg
    21721 tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc aggcattgaa gatacgcagc
    21781 ggcgcagcta tcagtgcttt tatacctttt ccacgcagac cagagatacg aatgaaatca
    21841 atttcgatgc catgttttgg cactaagtcc gcttccatac ggtcggcagt ccccagccag
    21901 cgaacttgcc aaccctgagc cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt
    21961 ccaccggttc cgcctgccat caccattaat cgctttcctt gagcactcat cgtgaacctc
    22021 gtacaaacgc ctgcgctttc tccagacgcg tttcataatc aatacgcaac agcatcatga
    22081 tggctgtcga cataatcagt aagctcgaac caccgtaact gatcagcggc aatgtcagac
    22141 ctttggtcgg taacatcccc gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc
    22201 agatgccaat agaacaggcg agaaaaccgg aaaaacggtg gtcaatttct aatgctttac
    22261 ggccaatcga catcgcgcga aaagcgacga agaataccat taaaagtgcc agcaccacac
    22321 cgacataccc cagttcttcg ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca
    22381 gatactccag tttttgtaca gagttaccta aaccttgtcc ccacagttcg ccgcgaccaa
    22441 acgccatcag cgattgcgtt aactgatagc cgctgccaaa gggatcttcc cacgggttcc
    22501 agaatgcggt aacacggcgg atacggtacg gttcggcgag tatcagcaac acaaccgctg
    22561 aaatgcccat accgataatg gcaatgaact gccacaattt cgctcccgcc aggaacaaca
    22621 tcgccagcgt agtcacaaac aacaccacca ccgtaccaag gtctggctgt gccagcagta
    22681 acactgccaa caccagaatc acgcccatcg gtttcaggaa gccgcgcagg ttattacgta
    22741 cttcgtcacc tttacgcacc agatagttgg cgatatagca aaacagcgac agttttgtca
    22801 gctcagcagg ctggatacgc agcaaaccga gatcgatcca acgcgatgcc cctttaaccg
    22861 agctccccac taccagcacg atcatcagca ggatgataga tccgagcagc atcgtggcac
    22921 tgtagcgttg ccagaactcc atcggcagac gcagcgtaat gatcgccaga ataaacgcca
    22981 aaatcagata gacaccatca cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc
    23041 ctatgggcat tgacgccgag gtcaccatga taaagccaat cgccgcgagg ccgaaggtca
    23101 gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt atctttttcc cgcgagccca
    23161 tcacccagcc ctttagcgcc gtggagatcc agaccaggat actgaatcct ggcaggcgcg
    23221 gcattttcag gcgagggaga gataaacgca tcaacctaac tccttcgcca gacgggcaaa
    23281 ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg ctggcacagg ccggggagag
    23341 tagaaccata tcgcccggct gaacacgcgg agccagcaag cgcatcgcct gttccatagt
    23401 ttcggtttgt tctgccactt ccgggcgtag cgccgccagc tgcacgccgt cacgaccgaa
    23461 acaatacaga cgtacgttat cgccattcag gtaacgcgcc agtgggctaa agtccgccga
    23521 tttaccatcg ccgcccagca acaaatgcag cgtgccgtct acgtgcaggc cattcagcgc
    23581 cgcttccgta ctgccgacgt tggtcgcttt cgaatcgtta atccaacgta cgccgttatg
    23641 ctccagcaca acttcaaagc gatgcggcag accagtgaat gtggttaacg ctttcaggct
    23701 gctggcacgc ggtaaccctg cggcatctgc cagcgccagc gccgccagcg cattggtgta
    23761 gttatgctgc ccggaaagtt tcatttcttt cacgttcagc actttctcgc ctttcacccg
    23821 cagccaggtt tcgccctgct gatgattcag gtgatagtca cccatgttga cgccaaagct
    23881 gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag gcatcatcag cattgacgac
    23941 acaaactttc gcattttcgt aaatacgcaa ttttgccgca cggtattgct gtaaaccaaa
    24001 cggatagcga tccatatgat cttcagtcac gttcagaatg gtcgctgcta ccgcctgtaa
    24061 gctggaggtg gtttccagct ggaagctcga cagttccagt acgtacagtt cgcactcatc
    24121 atccagcagc atcaacgcgg gcaggccaat attgccaccc acaccaacgt taacccccgc
    24181 cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat
    24241 cgccacaatc ggtgcttgtg cttcgcgaca gaacagctcg atatcgccaa cgatttcgat
    24301 tccggcatca gcggcagcgc ttaaggatgg atgcgccagt gcaataccgg gactggcgac
    24361 aatcagatct gccgccatca gccattcatc attcagactg cccgtgtggc gttctacggc
    24421 ttcgggtaat ttatccaggc caggcggtgt catacgcgta tccataacgc gcggcgtcac
    24481 accgcgagcg aggaaaaagt ccacgcagga aagcccggtg aggcccaggc cgataatgac
    24541 aacattttta ccctggtaat cagccatgat taacgtacct tcagcgttgc cagaccaatc
    24601 aggaccagca tcagcgaaat aatccagaaa cgcacaatga cgcgcggttc cggccagcct
    24661 ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta
    24721 aaggagccga cctgcaggat gacggaaagc gtttctacca cgaacacgcc ccccataatc
    24781 accagcagga attcctgacg cagcagtacg gcgataatgc ctaacgcacc acctaacgcc
    24841 agcgaaccta catcgcccat aaagacctgc gccggatagg tgttaaacca caggaagccc
    24901 agccctgccc cgactatcgc ggtacagaca ataaccagtt ccccggcgtg tcgcagatat
    24961 ggtatgtgta aatagctggc aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa
    25021 ccaccggcga caaatacggt cggcataatt gccagaccgt caagaccatc ggtcaggttt
    25081 accgcgttgc cagtacccac aatgacgaag taagccagca gaatgtagaa cagccccagt
    25141 tgcggcatca catctttaaa gaatgggacc accagctgcg ttgcgggcgt gtctttgcca
    25201 acaaggtaca gcgcgaaggc gacacccagc gcaatgaccg acatccagaa atacttccaa
    25261 cgagcgatca accctttggt gtctttacgc actactttgc gataatcatc aacaaagcca
    25321 ataacaccgt aacctaccag caccaccaac acgcaccaga cgtacggatt ggacgggtaa
    25381 gcccacagca gtacggagat cacaatcgcc gtcaggatca taatcccgcc catggtcggc
    25441 gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac gcaccacctg accaaaggaa
    25501 agtttttgca aatgagcaat catacgcggg cccatccaca atgagatgaa cagcgcggtc
    25561 agcaggctga cgatggcgcg aaacgtcaga taggaaaaga cgttaaagcc ggaataatat
    25621 ttgaccaaat gttcggccag ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta
    25681 cctcttccat ggcggcacta cgtgaaccct taactaaaat cgtaattacc tgttgctcag
    25741 caatcagtga tttaagacgc gtaattaacg cagttttctc agcaaaatgt tcgccaacgc
    25801 cgctggcggt gctgatagca tggctttgtt tacccacgct taacacgcgg tcaataccag
    25861 cagcttttgc cgcctcgccc acctgtacat ggcaggcttc gctttcagcg cccagttccg
    25921 ccatatcgcc caccaccagc acgcggtagc ccggcatttc agccagtacc tgaacagcgg
    25981 cggtcattga accgacattg gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca
    26041 gttgaatggg gaacaaacgg cctggaacag ctttcagatt tgccagcccc gctttgatag
    26101 catcaagcgt tgcgcccacg gacatggaga gcgcagcggc tgccagcgca ttcgcaatat
    26161 tgtgacgccc cggcaacggc agcagaacat ccacgctacc ggttggggtt tgcagggtaa
    26221 attccgtacc gtgcgaagtc acatggatat tggtggcggt gaaatcgctg ttggcggcat
    26281 taggtgagaa acgccacact ttgcgtgagc caattacgct ctgccagttc agccagtcgt
    26341 tgttgtcggc gttcataatg gcgataccgt tttccggcag gccgctaaag atttcacctt
    26401 tcgctttcgc gacacccgca agcgagccaa aaccttccag atgcgccgct gccaggttgt
    26461 tgaccagcgc agcttccggg cgagtcagac tcacagtcca gacaatttcg ccctgatggt
    26521 tcgcgccaag ttcaataact gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg
    26581 gtacaccgat gtcgttgttg agattgcctg ccgtataaag cgtgttgccg cactggctta
    26641 aaatcgccgc cgtcatctct ttaacggagg ttttgccgga ggaacctgtc agagcaacca
    26701 cgcgcgccgg aacttgctgg cgaacccatg cagccagttc accaaacgcc agacgcgtat
    26761 ccttgacgat taactgcggc aggtcgatat ccagcggacg gctaaccagt agtgcgcctg
    26821 cgccgccagc tttcgcctgg tcggcaaaat catgagcatc gaaacgttcg cctttcaggg
    26881 caacaaacag gcagcccggc gtcagttttc gggtatcagt ggttacagca tcaagggtga
    26941 tatctgcacc ttgcagttca ccgttgagaa tgtcggtaag ttggctaagg gttacgctaa
    27001 tcatgcaatc acccccagca gacgcgccac cgtgacgcga tcggagtagt ccagacgctg
    27061 attgccaaca atctggtaat cttcatggcc tttgcccgcg accagcacca catcgttctc
    27121 tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg tcttccatca ctttggcatg
    27181 tccggcatct aacattcccg ccagaatatc gttgatgatg gcacgcggct cttcggtacg
    27241 cggattatcg tccgtcacca ccgccacatc agcaaactct tcggcaattg cgcccatcag
    27301 tggacgctta cctttatcgc gatcgccacc acagccaaag acacaccaca gcttgcccgc
    27361 acagtgcaga cgcgccgcct gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc
    27421 caccaccacc gtcggtttgc ctggcgcagt gaacacttcc atacgtccgc aaaccggttg
    27481 cagacgagca gcggttttca gcagatcagc cagtggatag cccagcgcca gcagcgtcgc
    27541 cagcgcgagc agcaggttgc tgacgttaaa agcgcccatc agatggcttt caatttcgcc
    27601 atcgccccaa cttgagctaa agcgaatagt cgcaccgctg tcgtgatagt tcacttcggt
    27661 cgctttcaac cagcgtccgt gacagttcgg attgatatga tcttccattg ataccgcaac
    27721 tgcatccggc agttttgcca gccagcggcg gcctacttca tcgtcggcat taataatcgc
    27781 ctgaccgcaa tgatgttcag aataaagcag ccatttcgca gcttcgtagt gttccatatc
    27841 accatgataa tcaaggtgat cgcggcttaa gttagtaaag accgacgccg caaatttcaa
    27901 tgccgccaca cggtgctgta ccagcccgtg ggaggaaact tccattgcgc aaaaagtcgc
    27961 gtcctgatcc accagccccg ccagctcatg ctgaacatca actgccgaac cggtggtatt
    28021 ttctgtcggg atcactttcc ccagcagacc gttaccaacg gtgcccatta ccgcgctggt
    28081 ttcgccaagc agttggctcc actgcgccag cagctgggtc gtcgtggttt tgccgttggt
    28141 gcccgttacg cccacgagac gtaaattgtc agagggttca tggtaaaagc ggcccgccag
    28201 tgcagataaa cgctcgttga gctggctgag atagatgacc ggtacgccgt gcatttcacg
    28261 gatttcaccg tcggtcgcct cacctttcgc ctctgcaata atggcagcca caccttgcgc
    28321 tatcgcctgc gggatatatc gacgcccgtc cgcctgatga cctactacag ctacaaagag
    28381 atcgcccgcc gcagccacac ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc
    28441 gtctggcacc cacggagcaa gaaggtcgcg caaattacga tctgccacct gtcccctcgc
    28501 cttgattaat cacaaattca tttttatcgc ccgttgtcag cgcatccggc tcgatgttca
    28561 tggttcgcaa tacgccgccc atgatggcac caaagaccgg cgcggaaacg gcgccgccgt
    28621 agtatttacc cgcctgcgga tcgttgataa caacaaccag cgcgaagcgc ggctgactcg
    28681 caggcgcaac acctgcggtg taagcaatat atttattgat gtagcgaccg tccggcccga
    28741 cctttttcgc ggtaccggtt ttaatggcaa tacgatagcc tttaatcgcc gccttcacgc
    28801 cgccgccacc cggtagcgcc acgctttcca tcatatgcac cacagtgcgg acaatagatt
    28861 ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat
    28921 aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa ctgtaacgga gttaccatta
    28981 gcccgtagcc gaaagagaag gtggccctct ctatgtcaga ccaccgttgt ttttgaggat
    29041 ataagccact gcgttctccg accaacccca aattggtcgc ttttcccagt ccaaaacgtg
    29101 agtaagtatc tactaacgct gaggacggca tcgctaacgc cagcttggaa acaccgacgt
    29161 tactcgactt ctgtaatact ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga
    29221 tttcgtggcc gttaattcga taaggaatgg tattgagtac cgagttttcc cgcaccacgc
    29281 cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt tgaacccggt tcaaacacgt
    29341 cggtgatggt acggttacgc atcgcctctt tcggcgtgcc gctcagattg ttagggttgt
    29401 atgacgggct gttagccatc gccagcactt caccggtgtt gacatccacc agcacggcgc
    29461 taccggactc agccttgtta aaggccaccg cgttgttcag ttcgcgataa accagcgcct
    29521 gcaggcgttc atcaatactc agcgccaggt tgtgcgcagc ctggctgtca gtagaagaaa
    29581 tatcttcaat tacgcgacca tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg
    29641 taagccattt atcgaaactc ttctcaacgc cctcaatacc ttgactatcg acgttagtaa
    29701 agccgatgag gtgagcagtc acttcgccgg atggataata acggcgagat tcttcacgca
    29761 gatgaattcc cggcagtttc agttttttga tgtagtccgc catgtcaggg tttacctgac
    29821 gcgccagata aataaagcgc cctttcgggt tggcgttaat gcgggcagaa agctgatcca
    29881 gcggaatatt gagcgcgtta gccagcgcct tccagcggtc accgacgctg ataccgccag
    29941 cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac cggcacgctc accgctaacg
    30001 ggcgaccaga acggtcagta atcatgccgc gggaggtgga aacttgctga acgcgaagag
    30061 aacgcatgtc gccctctttc accagcatat ccggggagat aacttgtaac cacgctacgc
    30121 gtccgagcag aaaagccagc gccaggagaa tacagccgca taacaacgca aaacgccaac
    30181 tgataaagtt ggcatgttct tcctgacgtt ttggtttctg cgttttcgcc gctgctttca
    30241 tgcgtcgcgt ttatccttat ttttgcacta cgatattttc ttgtgacgga tcaacatgct
    30301 gcatttgcag cttttccgtg gcgatccttt ccacccggct atggtcgccg agcgcattct
    30361 cttcaaggat caggttgcgc cattcaatgt ctaaagcatc tcgctccagc accagttgtt
    30421 cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt taccacggtc accgccgtca
    30481 aaataatgca aatgaacagg cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga
    30541 taacaccagg caatgcatgg cgctcgtggc ttcccatcga tcctttaact ttgcttagag
    30601 cttctgtcac tctgctgatc atgcgttcgt cctctctgca atacgcagaa ctgaactacg
    30661 ggcacgagga ttctcagcca cctcttcttc gcccggcatt aatttgccta gtgctcgcag
    30721 ctgacggcca cccagttttt tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg
    30781 cggaccgcgg ctgttttcac gcataaaacg tttcacaata cggtcttcca gcgagtggaa
    30841 gctgatgatc gaaagccgcc cacccggggc cagcacgttg agcgagcttt ttagcgcctg
    30901 ctctatctcc tccagttcac tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc
    30961 gggatgttta aacttatctt tcaccggcgt tgcagcagcc acgacttccg ccagttcttt
    31021 ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg gctcgggcaa tgcgtttggc
    31081 aaaacgctct tcaccatagg ttttcaatac ccaggcgata tcggcttctt ctgcggtttg
    31141 tagccattca gcggctgact gcccacgggt tgggtccata cgcatgtcca gcggaccatc
    31201 gcgcataaag gaaaagccgc gttcagcatc atcaagttgc ggtgaagaga caccaagatc
    31261 gaggagaatg ccgtcgatct tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc
    31321 ggaaaaaggt ccgtggatga tggagaagcg cggatcatca atagtcttcg caacggcgat
    31381 agcctgcggg tcgcgatcga tcgccagcaa acgcccctct tcgccaagct gcgagaggat
    31441 cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg tagatgccat caggacggat
    31501 attgaggcca ttaacggctt catccagcag caccgtagta tgtttatagt tttccatcat
    31561 tttatagaga caagtcctgc agtcgctccg ataagtctcc ggtagccaac tgctctgcgt
    31621 cgatatcttc cttgacctgt tgatgccagg ttgtttcatc ccacagctca aacttgttga
    31681 actgtccaac cagcatcact tcttttgtca gcccggcatg ttgccgcagt actggcgcga
    31741 ttaacaatcg acctgcgcca tccatctgac attcgctggc atgacctaac agtaggcgct
    31801 gcacacggcg ctcaaccggg ttcatgctcg acagacgcga taatttttgc tcgataattt
    31861 cccattcagg cagggggtaa agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca
    31921 tttgaccggc agcgttctca agcagctgtt cccgataacg ggtaggcact gataagcgcc
    31981 ctttgctgtc gagattgact aacgttgctc cccggaacat gccagcctca ccccttattc
    32041 ccactttgcc ccacaatttc ccactgaaag gagtttacgg agcggaggaa aagcttgtca
    32101 agccagtcac aaggcatacc gaggtaaaaa acccgatgtt tacggctaat atcagcgttg
    32161 tttattttac gtccaacgtg cgaagcaaat taacgttatg aatagctgta agaaaaaaac
    32221 cctcatcgtc atcaccgttt taaaatgact cactattttc cacagcaaat ataaagtgtc
    32281 agtttgcgac gcgagcggca ttttaggaca tatcttcccc ggttaacagt ccctgttgcg
    32341 tctgtgtagc gcttgatgcc agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat
    32401 cggcaattca tctctgcatg taacaaaata atgcaaaaac gcgctgtcga tatcacttaa
    32461 ttattgtgaa tttcagctgg tgagttaatt aactggcggg tttaaaagca tttatcaata
    32521 atagtcagga atagtcttat ttactttaag gatattgatg tccagtcccg tactctacgc
    32581 gccagagtga aattcacctg gcgcgtattt ttgttcgcag cttagctacg gctgagcaca
    32641 ccgcggcgat agagattacg tttaatgcgc gttaaaccag gttttggctt acgcggttcg
    32701 tccaggcttg ccaggacaat ctccagcaca cgctctgcga catcgcggtg acgttgagcc
    32761 actgccagca ccgggcactg taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt
    32821 gccaggtcag aaggcagttt accatcgcga cgtagcgtga catccatcac cccttgcaac
    32881 aacgcaaacg acgttgtgaa cagcgcctgc ggcatcggat gcgtttccag ccatttttcg
    32941 aataactggg cagccgcctc ccgctcatag ctgttggcat acaggaaatg cacttcgcgc
    33001 ggatcatctt tccaggcagt acggaaacct tgttcacgca ggaagctgac agaaagctcc
    33061 ggcagcgcgc caagataaag caccgtctcg gcgggaaact tacgtaactc ttccgccagc
    33121 atttcggcat catcctgatc ggcaccaacc acgctggtaa agtgttcacg atcgagggcg
    33181 cggtccagcg cgacaatcgg gaacgggtcg ttagcccagc gttgataaaa aggatgctca
    33241 ggaggcaacg acgtcgaaac aataatggca tcgacctgac gctgtaaaag gtgctcaatg
    33301 cagcgcattt cgttgtctgg ctgatcttct gagcaggcaa tcagcagttg ataaccccgt
    33361 tgccgcgcct ggcgttcaag atagttagcg atgcgggtat agctggtgtt ctccagatcg
    33421 gggatcacaa gaccaataga acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc
    33481 gggtggtaat tgtgctcacg caccacagcc atgacttttt caacggtttt gtcactcaca
    33541 cggtattgct tcgctttgcc gttaataaca tagcttgcag tggtccgcga cactcccgcc
    33601 agccgagcga tttcatccag tttcacaatt gccccttgcg taaaatgtaa aaaccatagc
    33661 cattgtactg gtatgggtta aattcattaa catctaagcg cagaaaatta actgcggcaa
    33721 ctgcttttat tccaggttcc cactgatttc gcaaaaaaag cccaacgtga catgttgggc
    33781 tgtaaattgc gcattgagat cattctcaac gcattatttt atcgccgcgc gaaagtccga
    33841 ccacaccaga gcgagcaact tccacaattt tcgccacatc gcgaatcgat gctaaaaatg
    33901 catcaagctt atcgctggtg cctgctaatt gaacggtata aagcgagggt gtgacatcaa
    33961 taatttgccc acggaatatt tccgtattac gtttaacttc gtcacgcccg taaccgctgg
    34021 cctgaatttt caccagcatg atttccctct caacatgcgc gccctgcccc aactcactca
    34081 cgcgcaagac atcgaccagc ttgtataatt gcttttcgat ctgctcaagc actttttcat
    34141 cgcccacggt ctggatggtc atacgcgata atgtcggatc gtcggttggc gcaacggtca
    34201 ggctttcaat gttgtagcca cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg
    34261 attcattttc gagtaagact gataatatcc ggcgcataat caggttctct ccgttttgct
    34321 taaccacatt tcatccattc cgcccccgcg aatctgcatc gggtagacgt gctcactgcc
    34381 atcgacggtg acatcaacaa ataccaggcg attattacgc acctgttcaa gcgcctcgct
    34441 aagtttgctt tccagctctt gcggatgaga gatctggatc ccgacgtgac cataggcttc
    34501 cgccagacgg acgaagtctg gtaaagactg catataagat tgtgaatgac gaccggaata
    34561 gatcatgtcc tgccactgct tcaccatccc caaatagcgg ttattaagat taaccaccag
    34621 caccggcagt tcatattgca acgctgtaga cagttcctga atgttcatct gaatactgcc
    34681 gtcgccagtg acgcaaacca cggtttcttc tggcagcgcc attttgacgc ccagtgccgc
    34741 aggtaaacca aagcccatag tgccgaggcc accggagttg atccaacgac ggggtttgtc
    34801 gaatggataa taaagcgcag caaacatctg gtgctgcccg acatcggacg taacgtaagc
    34861 gccccccttc gtcaaccgcc aaagagtctc gatcaccgcc tgcggtttaa tcttttcact
    34921 gtgagtgtca tatttcaggc actgacgagc gcgccactgt tcaatctgct gccaccagtc
    34981 gcggatctca tccagcggtt gatgggcgga ttcttgcgac aagagttcaa gcatttgttc
    35041 gaggacctgg cgagcatccc ccacaatcgg aatatccgca gtcacggttt tagaaatgga
    35101 agtaggatca atatcgatat gcagaacagt ggcatttggg cagtactttg ccagattgtt
    35161 agtggtgcga tcgtcaaagc ggacaccgac agcaaaaatc acatccgcgt tatgcatcgt
    35221 catattggct tcgtaggtac cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc
    35281 cggaaacgct cccagcccca tcaatgagga aacaacgggc agatttaacg cctccaccgt
    35341 ttctttcagc tgttgatggc agcccgccgt gattgcccca ccgcctacgt agacaaccgg
    35401 tttttttgcc gccaccagcg tttgcagagc gcgcttaatt tgccctttat gtccggtagt
    35461 agtgggattg taagaacgca tactgaccga ctccggccag acatagggta atttgttcgc
    35521 cggattaaga atatctttcg gtaaatcaac gaccactggt cctgggcgac cacttgccgc
    35581 cagccagaaa gcctttttca gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact
    35641 gtgtttaacc accggtcgcg aaatccccac catgtcgcac tcctgaaagg catcgtaacc
    35701 tatcaacgag gtcgctacct gcccggaaag gacaactaat ggaatggaat ccatataagc
    35761 ggtggcgatg ccggtaatcg cattggtcgc ccctggaccc gacgttacca gcacgacgcc
    35821 gacttccccg gtcgcgcgcg ccagaccatc ggccatatgc actgccgcct gctcatgacg
    35881 aactaaaaca tgaccgatac cacccacggt atgcaatgca tcgtaaatat caaggactgc
    35941 gcctccggga taaccgaata cttgtttaac gccctgatcg ataagcgatc ggacgaccat
    36001 ctcggctcca gacaacatct ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa
    36061 acacattaac cgtccgatct tgcttaagca attggcagcc agagacataa acgccgggta
    36121 ataagaataa acaggaggaa aggtgaaaag aaacgataaa atcctccatt acgaaggata
    36181 aaaatgataa taaatcggct aaatcccacc acttacagca atttctctat ttttttaatt
    36241 aatcctaaaa tagagagccc acacattcag aataaaattc tacactcaat aaaaaatgca
    36301 gaataaacca gacattcatg tctgacctat tctgcattca gttagcgttt gcaaattgag
    36361 actaattgct cttccatcca ctgatggcct ttatcacgcc cggcagcttc atgccaggag
    36421 agataacagg ttctgctatt tagttttaac ggcagcggta atacctgtaa ttctaaggat
    36481 tcagcgaact cttcagccag ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc
    36541 acgctaagta cgctcatcat tgccatgccc tgatacgcga tactggcttg cttatctacc
    36601 gtgtcatacc aaggttgact aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt
    36661 tcgttataaa catcatgttt cagtaacggg cccttaattg ttgggtgatt tttgctggct
    36721 accagcacca tttcatcttt aaataatggc acgctggtaa attcaggacg atggaagtct
    36781 tcataactaa tcacaaactc cgtttcctga taacgcagct gatgttcagt gttctgattt
    36841 aatgaagact tgaacataac atgtatattt ggcgcaatct gctcaatgtg attataaatc
    36901 tgcgaggtca gaatactgtc taacgggctg caaacacaaa gatgaaatac acgttcactg
    36961 ctcgcgggtt caaaacctga accaggcaat tcattttgta ctagttgcaa tgcctgacga
    37021 actgaaccaa aaagttgaaa tgcgcgagca gtcggttgaa taccacggcc ataacgaaca
    37081 aaaagctcgt cattaaacat caccttcagg cgtgcaacag cgttactgac cgcaggttgc
    37141 gacattccca gaacatgagc agcacgggta atgttttgct cctgcatcac ggcatcgaaa
    37201 acggttaata agttgagatc gaccatgcgt agctgtggct tgctgaactc cgccgtctct
    37261 ggatgatctg tttgtacctc tggcatactt aactccactg tcacacttaa ctccctttcc
    37321 cttattggaa tgcagagaaa taatcctcga gaatatgatt tatcatgcat ataaaataag
    37381 aaaaaggaaa atgagtaaaa ttaggaaaac ataaagatac gggttcttat aaaaacagaa
    37441 tccattgccg tggaaagcgt catttaccac agcataataa tccataatgt tcttgttgcg
    37501 taaacaatct aatcataact accgcga
//
LOCUS       DAZKIF010000032        37255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZKIF010000032 DAZKIF010000000
VERSION     DAZKIF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.032"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(293..505)
                     /gene="rpmE"
                     /locus_tag="ACHOTW_001650"
     CDS             complement(293..505)
                     /gene="rpmE"
                     /locus_tag="ACHOTW_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP9843779.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            708..2906
                     /gene="priA"
                     /locus_tag="ACHOTW_001651"
     CDS             708..2906
                     /gene="priA"
                     /locus_tag="ACHOTW_001651"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9843780.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3062..4087
                     /gene="cytR"
                     /locus_tag="ACHOTW_001652"
     CDS             3062..4087
                     /gene="cytR"
                     /locus_tag="ACHOTW_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP9843781.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4179..5138
                     /gene="ftsN"
                     /locus_tag="ACHOTW_001653"
     CDS             4179..5138
                     /gene="ftsN"
                     /locus_tag="ACHOTW_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP9843782.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5231..5761
                     /gene="hslV"
                     /locus_tag="ACHOTW_001654"
     CDS             5231..5761
                     /gene="hslV"
                     /locus_tag="ACHOTW_001654"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP9843783.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5771..7102
                     /gene="hslU"
                     /locus_tag="ACHOTW_001655"
     CDS             5771..7102
                     /gene="hslU"
                     /locus_tag="ACHOTW_001655"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP9843784.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7169..8095
                     /gene="menA"
                     /locus_tag="ACHOTW_001656"
     CDS             7169..8095
                     /gene="menA"
                     /locus_tag="ACHOTW_001656"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9843785.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8188..8673
                     /gene="rraA"
                     /locus_tag="ACHOTW_001657"
     CDS             8188..8673
                     /gene="rraA"
                     /locus_tag="ACHOTW_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP9843786.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            8733..9407
                     /locus_tag="ACHOTW_001658"
     CDS             8733..9407
                     /locus_tag="ACHOTW_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843787.1"
                     /translation="MQNIILLFIYLLLIAVNHFRYKFLLSGNAGEMILYFANVFFNPL
                     ALSFIIATIICITIKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGIT
                     VTVPSKQWVANIVKQGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHV
                     CDVQGFACAYQEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKM
                     MLSARQ"
     gene            9530..10156
                     /locus_tag="ACHOTW_001659"
     CDS             9530..10156
                     /locus_tag="ACHOTW_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843788.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(10195..10440)
                     /gene="zapB"
                     /locus_tag="ACHOTW_001660"
     CDS             complement(10195..10440)
                     /gene="zapB"
                     /locus_tag="ACHOTW_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP9843789.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            10866..11711
                     /gene="glpF"
                     /locus_tag="ACHOTW_001661"
     CDS             10866..11711
                     /gene="glpF"
                     /locus_tag="ACHOTW_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP9843790.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            11734..13242
                     /gene="glpK"
                     /locus_tag="ACHOTW_001662"
     CDS             11734..13242
                     /gene="glpK"
                     /locus_tag="ACHOTW_001662"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9843791.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            13472..14482
                     /gene="glpX"
                     /locus_tag="ACHOTW_001663"
     CDS             13472..14482
                     /gene="glpX"
                     /locus_tag="ACHOTW_001663"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9843792.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            14579..15325
                     /gene="fpr"
                     /locus_tag="ACHOTW_001664"
     CDS             14579..15325
                     /gene="fpr"
                     /locus_tag="ACHOTW_001664"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP9843793.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(15330..15758)
                     /gene="uspD"
                     /locus_tag="ACHOTW_001665"
     CDS             complement(15330..15758)
                     /gene="uspD"
                     /locus_tag="ACHOTW_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP9843794.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(15785..16084)
                     /gene="yiiS"
                     /locus_tag="ACHOTW_001666"
     CDS             complement(15785..16084)
                     /gene="yiiS"
                     /locus_tag="ACHOTW_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP9843795.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(16296..16736)
                     /gene="yiiR"
                     /locus_tag="ACHOTW_001667"
     CDS             complement(16296..16736)
                     /gene="yiiR"
                     /locus_tag="ACHOTW_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9843796.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            16837..17436
                     /gene="yiiQ"
                     /locus_tag="ACHOTW_001668"
     CDS             16837..17436
                     /gene="yiiQ"
                     /locus_tag="ACHOTW_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP9843797.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            17544..18311
                     /gene="tpiA"
                     /locus_tag="ACHOTW_001669"
     CDS             17544..18311
                     /gene="tpiA"
                     /locus_tag="ACHOTW_001669"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9843798.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(18366..19121)
                     /gene="cdh"
                     /locus_tag="ACHOTW_001670"
     CDS             complement(18366..19121)
                     /gene="cdh"
                     /locus_tag="ACHOTW_001670"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP9843799.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(19228..20217)
                     /gene="sbp"
                     /locus_tag="ACHOTW_001671"
     CDS             complement(19228..20217)
                     /gene="sbp"
                     /locus_tag="ACHOTW_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP9843800.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(20536..21498)
                     /gene="pfkA"
                     /locus_tag="ACHOTW_001672"
     CDS             complement(20536..21498)
                     /gene="pfkA"
                     /locus_tag="ACHOTW_001672"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9843801.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(21679..22581)
                     /gene="fieF"
                     /locus_tag="ACHOTW_001673"
     CDS             complement(21679..22581)
                     /gene="fieF"
                     /locus_tag="ACHOTW_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP9843802.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(22730..23230)
                     /gene="cpxP"
                     /locus_tag="ACHOTW_001674"
     CDS             complement(22730..23230)
                     /gene="cpxP"
                     /locus_tag="ACHOTW_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP9843803.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            23380..24078
                     /gene="cpxR"
                     /locus_tag="ACHOTW_001675"
     CDS             23380..24078
                     /gene="cpxR"
                     /locus_tag="ACHOTW_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP9843804.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            24075..25448
                     /gene="cpxA"
                     /locus_tag="ACHOTW_001676"
     CDS             24075..25448
                     /gene="cpxA"
                     /locus_tag="ACHOTW_001676"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP9843805.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(25554..26228)
                     /gene="yiiM"
                     /locus_tag="ACHOTW_001677"
     CDS             complement(25554..26228)
                     /gene="yiiM"
                     /locus_tag="ACHOTW_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP9843806.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(26377..27360)
                     /gene="kdgT"
                     /locus_tag="ACHOTW_001678"
     CDS             complement(26377..27360)
                     /gene="kdgT"
                     /locus_tag="ACHOTW_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP9843807.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(27620..28240)
                     /gene="sodA"
                     /locus_tag="ACHOTW_001679"
     CDS             complement(27620..28240)
                     /gene="sodA"
                     /locus_tag="ACHOTW_001679"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP9843808.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            28525..29559
                     /gene="rhaT"
                     /locus_tag="ACHOTW_001680"
                     /pseudo
     CDS             28525..29559
                     /gene="rhaT"
                     /locus_tag="ACHOTW_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(29556..30404)
                     /gene="rhaR"
                     /locus_tag="ACHOTW_001681"
     CDS             complement(29556..30404)
                     /gene="rhaR"
                     /locus_tag="ACHOTW_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP9843809.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(30478..31314)
                     /gene="rhaS"
                     /locus_tag="ACHOTW_001682"
     CDS             complement(30478..31314)
                     /gene="rhaS"
                     /locus_tag="ACHOTW_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP9843810.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            31602..33071
                     /gene="rhaB"
                     /locus_tag="ACHOTW_001683"
     CDS             31602..33071
                     /gene="rhaB"
                     /locus_tag="ACHOTW_001683"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9843811.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            33068..34327
                     /gene="rhaA"
                     /locus_tag="ACHOTW_001684"
     CDS             33068..34327
                     /gene="rhaA"
                     /locus_tag="ACHOTW_001684"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9843812.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            34502..35326
                     /gene="rhaD"
                     /locus_tag="ACHOTW_001685"
     CDS             34502..35326
                     /gene="rhaD"
                     /locus_tag="ACHOTW_001685"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9843813.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            35336..35650
                     /gene="rhaM"
                     /locus_tag="ACHOTW_001686"
     CDS             35336..35650
                     /gene="rhaM"
                     /locus_tag="ACHOTW_001686"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9843814.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(35691..37085)
                     /locus_tag="ACHOTW_001687"
     CDS             complement(35691..37085)
                     /locus_tag="ACHOTW_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP9843815.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCCIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         9217 a         9574 c         9499 g         8965 t
ORIGIN      
        1 acgtcactcc gccggggcac accgaacagg ccacgccggt gggggcacca atgcgcaccc
       61 cggctgaacc ctgaacaatg ctaccgccat actgcgtcat gtcgccctga cgcgccgccg
      121 gttttccgct catcgtaaaa tccttttgaa ataaataaga accagaagcc tagcgccagt
      181 caacaacagg aaatgataat gagttaacac cccggaattt ttcagaattt ataaaaggca
      241 aattacaggc acaaaaaaag cgccgtgcgg cgcttttttc ggaaatccgg tcttatttgc
      301 tgcccgggat gttgaaacgc ttgttgaagc ggtcaacacg gccaccggta gcaacatcac
      361 gctgtttgcc agtgaagaac gggtggcact tgctgcacac gtcgaggttc aggtcatgac
      421 caacggtgga gcggattttc attacgttac cgcaagagca gctagcagta atttcttcgt
      481 atttcgggtg aatatctttt ttcatgggaa aacctcggtt taaggccgcg tcgctcttcc
      541 agccctaacg ccagacacca cgcgatgtta aaagtatagc ttcaatacga tcatttcgta
      601 cgaaggcgcg aaatcataca gaaattaacc agcatatgca aactgatccg cactcttcta
      661 cggcaatgtg tatactaacc caccgaattt caagtcagga tgatgctatg cccgttgccc
      721 acgttgcctt gcccgttccg cttcctcgta cctttgacta tctcctgcca gaaggcatgg
      781 cggttaaagc cgggtgtcgc gtgcgcgtgc cgtttggcaa acagcaggag cgcatcgggg
      841 ttgtggtgtc agttagcgac gtcagtgagc tgccgcttaa cgagctaaaa gcggtagtcg
      901 aagtgctgga tagtgagccg gtgtttactc actccgtctg gcgattgctg ctctgggcgg
      961 cggattacta tcatcatccg attggcgatg tgctgtttca tgccttgccg attttactgc
     1021 gccaggggcg gcctgcggcg aacgcgccga tgtggtactg gtttgccact gaacaaggcc
     1081 atgcggtgga tctgaacagc ctgaaacgtt cgccaaagca acaacaggca ctggcggcat
     1141 tgcggcaagg caaaatctgg cgcgaccagg ttgccacgct tgaatttaat gatgccgcgt
     1201 tacaggcgct gcgcaaaaaa ggtctgtgtg atttagcaag tgaaacacca gagtttagcg
     1261 actggcgaac gaactatgcc gtttctggtg aacggttgcg attgaatacc gaacaggcca
     1321 ccgccgttgg cgcaattcat agcgcggcgg ataccttctc tgcttggctg ctggcgggcg
     1381 ttacaggttc cggtaaaaca gaggtttatt tgagcgtact ggaaaacgtg ctcgctcagg
     1441 gcaaacaggc gctggtgatg gtgccggaaa tcggcctgac accgcaaact atcgcccgtt
     1501 ttcgtgaacg ttttaatgcc cccgtagaag ttctgcattc cggcctgaac gacagcgagc
     1561 gtctttcggc gtggttgaaa gcgaaaaatg gtgaggcggc gattgtcatc ggcacccgct
     1621 ccgcgctgtt tacgccgttt aaaaatctcg gtgtgattgt cattgatgaa gagcacgaca
     1681 gttcctacaa gcagcaggaa ggctggcgct atcatgcccg cgacctggcg gtttatcgtg
     1741 cgcacagcga gcaaatcccg attattcttg gctccgcaac acccgcactg gaaacgttat
     1801 gcaacgtcca gcagaaaaaa taccgcctgc tgcgcctgac gcgtcgggcg gggaatgcgc
     1861 gtccggcaat tcaacatgtg ctggatttaa aaggtcagaa ggtgcaggcg gggctggctc
     1921 cggcgttaat cacccgtatg cgccagcatt tacaggccga caaccaggtt atcctctttc
     1981 ttaaccgccg tggctttgcg cctgccttgc tgtgccacga ctgcggctgg attgccgaat
     2041 gtccacgttg cgatcactac tacacgctgc atcaggcgca acagcatttg cgctgtcacc
     2101 actgtgacag ccaacgcccg gtgccgcgcc agtgcccttc ctgcggttcc acgcacctgg
     2161 tacccgtggg gctgggcacc gaacagcttg aacagacgct cgcgccgtta ttccctggtg
     2221 tgcccatttc tcgtatcgac cgcgatacca ccagccgcaa aggagcgctg gaacaacaac
     2281 tggcggaagt gcatcgcggc ggcgcgagga tattgattgg cacgcaaatg ctggcgaaag
     2341 gtcaccactt tccggatgtg acgctggtag cgttactgga tgtggacggc gcgctgtttt
     2401 ctgccgattt tcgctcggca gagcgttttg cccagctata cacccaggta gcaggccgtg
     2461 ccgggcgtgc gggtaaacag ggcgaagtgg tgctgcaaac gcatcatccg gaacacccgc
     2521 tattgcaaac gttgctctat aaaggctacg acgcttttgc cgaacaggca ctggctgagc
     2581 ggcgaatgat gcaactgccg ccgtggacca gccatgtgat tgtgcgtgcg gaagatcata
     2641 acaatcagca cgcgccattg ttcctgcaac aactgcgtaa tctgatcctc tccagcccgc
     2701 tggcagacga caaattgtgg gttctcggtc cggttccggc actggcacct aaacgtggcg
     2761 gtcgctggcg ctggcagata ttgttgcagc acccttcccg cgtgcgcttg caacacatca
     2821 ttaacggtac gctggcactc atcaatacaa taccggattc ccgtaaggtg aaatgggtgc
     2881 tggatgttga tccgattgag ggttaaaccg ctcacgatgc gaggcggatc gaaaaattca
     2941 atattcatca cacttttcat gaaaattctg taaccgtttt cacgcgctat ctgctaaaaa
     3001 tgttgccgat gtgaagtaaa catggatgta gtacgcctga cgtgccaggc gaggagtgag
     3061 tgtgaaagcg aagaagcagg aaactgccgc gaccatgaaa gacgttgccc tcaaggcaaa
     3121 agtctctaca gcgaccgtct cccgagcatt aatgaatccc gataaagtct cccaagccac
     3181 ccgtaatcgg gttgaaaaag cggcccgtga agtgggttat ttaccgcagc ctatggggcg
     3241 caacgtcaag cgtaatgaat cccgcaccat tctggtgatt gtcccggata tctgcgatcc
     3301 cttctttagc gaaattattc gcggtatcga agttacggcg gcaaatcacg gatatctggt
     3361 gctgattggc gactgtgcgc atcaaaatca gcaggaaaaa acctttatcg atttgatcat
     3421 caccaagcaa attgatggca tgttgctgct gggttcaagg ctgccgtttg atgccagcat
     3481 tgaggaacag cgtaatctgc cgccgatggt gatggcgaac gaatttgcac cggaactgga
     3541 gctgcctacc gttcatatcg acaatctgac cgccgcattt gatgcagtaa attatttata
     3601 tgagcaaggg cataaacgga ttggctgtat agccggtccc gaagagatgc cgctgtgtca
     3661 ctaccgcctg caaggttacg ttcaggcgct gcgtcgctgc ggcattatgg ttgatccgca
     3721 atacatcgcc cgtggcgact tcaccttcga agccggaagc aaagcgatgc agcagctgct
     3781 tgatcttcca caaccgccta ctgctgtctt ctgccatagc gatgtgatgg cgctcggcgc
     3841 actttctcag gcaaaacgcc aggggctgaa agtcccggaa gacctttcca taatcggttt
     3901 tgataacatc gacctgacgc aattttgtga tccgccgctg acaaccatcg cgcagccgcg
     3961 ttacgaaatc ggtcgggaag ctatgctgtt attgcttgat caaatgcagg ggcaacacgt
     4021 tggcagtggc tctcgtttaa tggactgcga acttatcatc cggggatcaa cacgcgcgtt
     4081 accttaaagt aaacctttaa gactgccgtg actggtcaaa ggcccgccgc ttaagtaaca
     4141 tggcgggctg acgaacgaat aaatacagcg aaacgatagt ggcacaacga gattatgtac
     4201 gccgcagcca accggcacct tcgcggcgaa aaaagagcac ctcacggaaa aagcaacgaa
     4261 atctgcctgc ggtttctccc gctatggtcg ctattgctgc cgccgttctt gtgaccttta
     4321 tcggtggtct gtacttcatt acgcatcaca agaaagaaga gtccgagacg ctgcaaagcc
     4381 agaaagtgac cggaaacgga ctaccaccaa aaccagaaga acgctggcgc tacatcaaag
     4441 agctggaaag tcgccagccg ggtgtgcgtg cgcccacaga accttctgcc ggtggtgaag
     4501 tgaaaacgcc ggagcaactg acaccagaac aacgtcagct tcttgaacaa atgcaggctg
     4561 atatgcgcca gcagccaacg cagctggttg aagttccgtg gaatgaacag acgccggaac
     4621 agcgtcagca aacgctacag cgccaacgtc aggcgcagca attagcggaa cagcaacgcc
     4681 tggctcagca gtcgcgtacg actgaacaaa gctggcagca gcagacgcgt acgtcgcaag
     4741 ccgcgccggt acaggcgcaa ccgcgccagt ctaaaccggc ttctactcag cagccgtacc
     4801 aggatctgct gcaaacccct gcgcacacga ccgcgcagtc gaagccgcag caagctgcgc
     4861 cggttactcg tgccgctgac gcgccaaaac cgacggcgga gaaaaaagac gaacgccgct
     4921 ggatggtgca gtgcggttcg ttcagaggcg cggaacaggc agagacggta cgtgctcagc
     4981 tggcgttcga aggctttgac tcaaaaatta ccaccaacaa tggctggaat cgtgtggtca
     5041 ttggcccggt gaaaggcaaa gagaacgcag acagcaccct caatcggttg aagatggcgg
     5101 gtcatacaaa ctgcattcgg ctcgccgccg ggggttgaaa ccctcaaaat cccccccatc
     5161 tataattgca ttatgccccg tacttttgta cggggtttgt actctgtatt cgtaaccaag
     5221 gggtcagctc gtgacaacta tagtaagcgt acgccgtaac ggccatgtgg tcatcgctgg
     5281 tgatggtcag gccacgttgg gcaataccgt aatgaaaggc aacgtgaaaa aggtccgccg
     5341 tctgtacaac gacaaagtca tcgcgggctt tgcgggcggt actgcggatg cttttacgct
     5401 gttcgaactg tttgaacgta aactggaaat gcatcagggc catctggtca aagccgccgt
     5461 tgagctggca aaagactggc gtaccgatcg catgttgcgc aaacttgaag cactgctggc
     5521 agtcgcggat gaaactgcat cgcttatcat caccggtaac ggtgacgtgg tgcagccaga
     5581 aaacgatctt attgctatcg gctccggcgg cccttacgcc caggctgcgg cgcgtgcact
     5641 gttagaaaat actgaactta gtgctcgtga aattgctgaa aaggcgttgg atattgcagg
     5701 cgacatttgc atctatacca accatttcca caccatcgaa gaattaagct acaaagcgta
     5761 aggatctccc atgtctgaaa tgaccccacg cgaaatcgtc agcgaactgg ataagcacat
     5821 catcggccag gacaacgcca agcgttccgt ggcgattgct ctgcgtaacc gctggcgtcg
     5881 catgcagctc aacgaagagc tgcgccatga agtgaccccg aaaaatatcc tgatgatcgg
     5941 cccgaccggt gtcggtaaaa ctgaaatcgc ccgtcgtctg gctaagctgg cgaatgcgcc
     6001 gttcatcaaa gttgaagcga ccaaattcac cgaagtgggc tacgtcggta aggaagtgga
     6061 ttctattatt cgcgatctga ccgatgccgc cgtgaaaatg gtacgcgtcc aggctatcga
     6121 gaaaaaccgt tatcgcgctg aagaactggc agaagaacgt attctcgacg tgctgatccc
     6181 acctgctaaa aacaactggg gacagaccga acagcagcag gaaccgtccg ctgctcgtca
     6241 ggcattccgc aaaaaactgc gtgaaggcca gcttgatgac aaagaaatcg agatcgatct
     6301 tgccgcagca ccgatgggcg ttgaaattat ggctcctcca ggcatggaag agatgaccag
     6361 ccagctgcag tccatgttcc agaacctggg cggtcagaag caaaaagcgc gtaagctgaa
     6421 aatcaaagac gccatgaagc tgctgattga agaagaagcg gcgaaactgg tgaacccgga
     6481 agagctgaag caagacgcta tcgacgctgt tgagcagcac gggatcgtgt ttatcgacga
     6541 aatcgacaaa atctgtaagc gcggcgagtc ttccggtccg gatgtttctc gtgaaggcgt
     6601 tcagcgagac ctgctgccgc tggtagaagg ttgcaccgtt tccaccaaac acgggatggt
     6661 caaaactgac cacattctgt ttatcgcttc tggcgcgttc cagattgcga aaccgtctga
     6721 cctgatcccg gaactgcaag gtcgactgcc aatccgcgtt gaactgcagg cactgaccac
     6781 cagcgacttc gagcgtattc tgaccgagcc gaatgcctct atcaccgtgc agtacaaagc
     6841 actgatggcg actgaaggcg taaatatcga gtttaccgac tccggtatta aacgcatcgc
     6901 ggaagcggca tggcaggtga acgaatctac cgaaaacatc ggtgctcgtc gtttacacac
     6961 tgttctggag cgtttaatgg aagagatttc ctacgacgcc agcgatttaa gcggtcaaaa
     7021 tatcactatt gacgcagatt atgtgagcaa acatctggat gcgttggtgg cagatgaaga
     7081 tctgagccgt tttatcctat aatcgcgttc aatcattttc atcattgttt gatggggctg
     7141 aaaggcccca tttttattgg cgcgtattat gactgaacaa caaattagcc gaactcaggc
     7201 gtggctggaa agtttacgac ctaaaaccct ccccctcgcc tttgctgcaa ttatcgtcgg
     7261 gacggcgctg gcatggtggc aaggtcattt tgatccgctg gtcgccctgc tggcactgat
     7321 taccgccggg ctattacaga tcctttctaa cctcgccaat gattacggcg atgcggtaaa
     7381 aggcagcgat aaacctgacc gtatcggacc gctacgcggc atgcaaaaag gggtcattac
     7441 ccagcaagag atgaaacggg cgctcattat taccgtcgtg ctcatctgtc tctccgggct
     7501 ggcactggtt gcagtggcgt gccatacgct ggccgatttt gtcggtttcc tgattcttgg
     7561 cgggttgtcg atcattgccg ctatcaccta caccgtgggc aatcgtcctt atggttatat
     7621 cggtctgggt gatatttccg tactggtttt ctttggctgg ttaagcgtca tggggagctg
     7681 gtatttacag gctcatacat tgattccggc actgatcctt ccggcgaccg catgcggcct
     7741 gctggcaacg gcagtactga acattaataa cctgcgtgat atcaatagcg accgcgaaaa
     7801 cggcaaaaac acgctggtgg tgcgcttagg tgaagtgaac gcgcgtcgtt atcatgcctg
     7861 cctgctgatg ggctctctgg tatgtctggc gctgtttaat ctcttttcgc tgcatagcct
     7921 gtggggctgg ctgttcctgc tggcggcacc gttgctggtg aagcaagccc gttatgtgat
     7981 gcgggaaatg gacccggtgg cgatgcgacc gatgctggaa cgcactgtca agggagcgtt
     8041 actgactaac ctgctgtttg ttgtagggat attcctaagc cagtgggcag cataactgac
     8101 aaatatcaat taacaattga tgattttgcc aacagaccac atagcgcgat atactgaaaa
     8161 ttctcgcagc aactgaatgt taagcctatg aaatacgata cttccgagct ttgtgacatc
     8221 tatcaagaag atgttaacgt cgtggaaccg ctgttctcca actttggcgg acgggcgtcg
     8281 tttggcggac aaataatcac ggtaaaatgt ttcgaggaca acgggttact gtacgatctg
     8341 ctcgaacaga atggccgtgg tcgtgttctt gtcgtcgatg gcggtggttc tgttcgtcgc
     8401 gcactggtcg atgctgaact ggcgcgtctg gcagtacaaa atgaatggga aggtctggtc
     8461 atttacggcg cggtgcgtca ggtagatgac ctggaagagt tggatatcgg catccaggcg
     8521 atggcggcaa ttccggttgg tgccgctggc gaaggcattg gcgaaagcga tgtccgcgtc
     8581 aattttggcg gtgttacctt cttctccggc gaccatcttt atgccgacaa taccgggatt
     8641 attctttcag aagatccgct ggatattgaa taagtatctg ccatgcgtta aaaggggaat
     8701 taaattcccc ttttttatac atggacgtat tcatgcagaa tattattctt ttatttatat
     8761 atttattact tatagccgtt aaccatttcc gctacaaatt tcttttatct ggcaacgcgg
     8821 gtgaaatgat tttatatttt gctaacgtat tttttaaccc acttgcctta tcatttatta
     8881 tcgccacaat catctgtata acgataaaaa aaagaagcag ccgcagcttt attcgcggca
     8941 cttgttggtt aatgggatta tttcttatct atagcagcta tacggtgtgg gaacgacaca
     9001 gcatatggga ttatactttc ccggagccag gtatcaccgt cacggttccg agcaaacagt
     9061 gggttgccaa tatagtaaaa caaggaccaa ccctggtgac ccgggatgcc catgctattt
     9121 tagctattgt tgccttctca caaaatgaac tcggtgtgca ttcaatcgag gaacttaccg
     9181 cgactcaaaa cggaactgca gaaatgcatg tttgtgatgt tcagggattt gcctgcgctt
     9241 atcaggaagg agtgcaaaca atgagtgatg gcaaagtaag acatgctatt ttcatgactc
     9301 tactggataa cactaatttg atacaactgg tcgcagctat cgacccagat tatttggacg
     9361 aatatcaaga agaagtaatg aaaatgatgc tttctgctcg tcaatgatct gatgtgcatg
     9421 ttaataataa tggattgata tgagatacgt tatcttatta atactatgga taataactgt
     9481 ttttcttagt catcttcagc acagtaactt gcttactgga gatccaggga tggttggcga
     9541 gtatatcggt tcagtacttc ttggaccgtt attacttccc gtgcttgtgt caggtattct
     9601 gtgcacattc actaaaaaaa cgcgtaactt cgccagtttt actcgcgggt gctgttgggt
     9661 attgggagtc ctgcttttat ccaacgtcgg caatacgttt cgtctgttca ccccgtggca
     9721 ctatacgttt gagaaggcag ctatttccgt gactgtccca aaccgacact ggaatacggt
     9781 ttctattagt actgataaaa ccattgatat aagaagtgaa gataatagcg tctttatttc
     9841 tgctttccgc ctgcccgcag gacgtagcgc cgatgactcg ctggaagagt taaagaagat
     9901 gcagcgagac aatctcaagg atcaatacaa tgaagaaacc ttccagtttc atgattgtaa
     9961 tgccaaacat ttcacctgta agtatcagga tgtgttgata aattttgatg gtcagcagaa
    10021 aagaaccatc agtgtttatc ttgaagacac ccccagggct gtggggatca ttgcattaat
    10081 ggagccagat acggcagata aatatcgcca acaggcgatg gagattatgt tgtctgctaa
    10141 aaatacagtg aagtaaaaaa taaggcgcct tgcggcgcct ctgcgtggaa gagattaaac
    10201 ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct gctgttcttt
    10261 cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt gaacttcctg
    10321 cgacaatgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca gagtgatggt
    10381 atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctaatg acattgtcat
    10441 acctctcctg aattgcaagg cgttgatgga taaaaaatcc tcgtcccgat taccggtgac
    10501 gccttaataa atacgagcgc actttagtta gctccgattg tatgaagccg cgccatcgct
    10561 gtccagcggc acgccttgca gattacggtt tgccacactt ttcatccttc tcctggtgac
    10621 ataatccaca ccaatcgaaa atgttaataa atttgttgcg cgaatgttct aacaaacatg
    10681 catcatgtac aatcagatgg aataaatggc gcgataacgc tcattttatg acgaagcaca
    10741 cacattttaa gttcgatatt tctcgttttt gctcgttaac gataagttta cagcatgcct
    10801 acaagcatcg tggaggtccg tgactttcac gcatacaaca aacattaact cttcaggatc
    10861 cgattatgag tcaaacatca accttgaaag gccagtgcat tgctgaattc ctcggtaccg
    10921 ggttgttgat ttttttcggt gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt
    10981 ttggtcagtg ggaaatcagt gtcatttggg gactgggggt ggcaatggcc atctacctga
    11041 ccgcaggggt ttccggcgcg catcttaatc ccgctgttac cattgcattg tggctgtttg
    11101 cctgtttcga caagcgcaaa gttattcctt ttatcgtttc acaagttgcc ggcgctttct
    11161 gtgctgcggc tttagtttac gggctttact acaatttatt tttcgacttc gagcagactc
    11221 atcacattgt tcgcggcagc gttgaaagtg ttgatctggc aggcactttc tctacttacc
    11281 ctaatcctca tatcaatttt gtgcaggctt tcgcagttga gatggtgatt accgctattc
    11341 tgatggggct gatcctggcg ttaacggacg atggcaacgg tgtaccacgc ggccctttgg
    11401 ctcccttgct gattggtcta ctgattgcgg tcattggcgc atctatgggc ccattgacgg
    11461 gttttgccat gaacccagcg cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct
    11521 ggggcaatgt cgcctttacc ggcggcagag acattcctta cttcctggtg ccgctttttg
    11581 gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt
    11641 tgccttgcga tatctgtgtt gtggaagaaa aggagaccac aactccttca gaacaaaaag
    11701 cttcgctgta atatgactac gggacaatta aacatgactg aaaaaaaata tatcgttgcg
    11761 ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt
    11821 agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac caggctgggt agaacacgac
    11881 ccaatggaaa tctgggccac ccaaagctcc acgctggtag aagtgctggc gaaagccgat
    11941 atcagttccg atcaaattgc agctatcggt attacgaacc agcgtgaaac cactattgtc
    12001 tgggaaaaag aaaccggcaa gcctatctat aacgccattg tctggcagtg ccgtcgtacc
    12061 gcagaaatct gcgagcattt aaaacgtgac ggtttagaag attatatccg cagcaatacc
    12121 ggtctggtga ttgacccgta cttttctggc accaaagtga agtggatcct cgaccatgtg
    12181 gaaggctctc gcgagcgtgc gcgtcgtggt gaattgctgt ttggtacggt tgatacgtgg
    12241 cttatctgga aaatgactca gggccgtgtc catgtgaccg attacaccaa cgcctctcgt
    12301 accatgttgt tcaacatcca taccctggac tgggacgaca aaatgctgga agtgctggat
    12361 attccgcgcg agatgctgcc agaagtgcgt cgttcttccg aagtgtacgg tcagactaac
    12421 attggcggca aaggcggcac gcgtattcca atctccggga tcgccggtga ccagcaggcc
    12481 gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga agaacaccta tggcactggc
    12541 tgctttatgc tgatgaacac tggcgagaaa gcggtgaaat cagaaaacgg cctactgacc
    12601 accatcgcct gcggcccgac tggcgaagtg aactatgcgt tggaaggtgc ggtgtttatg
    12661 gcaggcgcat ccattcagtg gctgcgcgat gagatgaagt tgattaacga cgcctacgat
    12721 tccgaatatt tcgccaccaa agtgcaaaac accaatggtg tgtatgtggt tccggcattt
    12781 accgggctgg gtgcgccgta ctgggacccg tatgcgcgcg gggcgatttt cggtctgact
    12841 cgtggggtga acgctaacca cattatacgc gcgacgctgg agtctattgc ttatcagacg
    12901 cgtgacgtgc tggaagcgat gcaggccgac tctggtatcc gtctgcacgc cctgcgcgtg
    12961 gatggtggcg cagtagcaaa caatttcctg atgcagttcc agtctgatat tctcggcacc
    13021 cgcgttgagc gcccggaagt tcgcgaagtc accgcattgg gtgcggccta tctcgcaggc
    13081 ctggcggttg gcttctggca gaacctcgac gagctgcaag agaaagcggt gattgagcgc
    13141 gagttccgtc caggcatcga aaccactgag cgtaattacc gttacgcagg ctggaaaaaa
    13201 gcggtcaaac gcgcgatggc gtgggaagaa cacgacgagt aatgtcaggc acctgaatcg
    13261 attgcctgat gctgatcgcc tgatgcgacg cttacgcgtc ttatcaggcc tacgagccaa
    13321 tgccattttg tgtaggcggg ataaggcgtt cacgccgcat ccggcatcac gcagactctg
    13381 aaaccgtgcg ggggcaaccc cgcacacatc aataatccca cccttcccct gtgctacact
    13441 tcgcgccatt ccttactgct tagagtttgc tatgagacga gaacttgcca tcgaattttc
    13501 ccgcgtcacc gaatctgcgg cgctggctgg ctacaaatgg ttaggacgcg gcgataaaaa
    13561 caccgcggac ggcgcggcgg taaacgccat gcgtattatg ctcaaccagg tcaacattga
    13621 cggcaccatc gtcattggtg aaggtgaaat cgacgaagca ccgatgctct acattggtga
    13681 aaaagtcggt actggtcgcg gcgacgcggt agatattgct gttgatccga ttgaaggcac
    13741 gcgcatgacg gcgatgggtc aggctaactc gctggcggtg ctggcagtag gcgataaagg
    13801 ctgtttcctc aacgcgccag atatgtatat ggagaagctg atcgtcggac cgggagccaa
    13861 aggcaccatt gatctgaacc tgccgctggc ggataacctg cgcaatgtcg cggcggcgct
    13921 cggcaaaccg ttgagcgaac tgacggtaac gattctggct aaaccacgcc acgatgccgt
    13981 tatcgctgaa atgcagcaac tcggcgtacg cgtatttgct attccggatg gcgacgttgc
    14041 ggcctcaatt ctcacctgta tgccagacag cgaagttgac gtgctgtacg gaattggtgg
    14101 cgcgccggaa ggcgtggttt ctgcggcggt gatccgcgca ttagatggcg acatgaacgg
    14161 tcgtctgctg gcgcgtcatg acgtcaaagg cgacaacgaa gagaatcgtc gcattggcga
    14221 gcaggagctg gcacgctgca aagcgatggg catcgaatcc ggtaaagtat tgcgtctggg
    14281 cgatatggcg cgcagcgata acgtcatctt ctctgccacc ggtattacca aaggcgatct
    14341 gctggaaggc attagccgca aaggcaatat cgcgactacc gaaacgctgc tgatccgcgg
    14401 caaatcacgc accattcgcc gcattcagtc tatccactat ctggatcgca aagacccgga
    14461 aatgcaggtg cacatcctct gattgatttg atcgattgag ccttccagtc cttcgggact
    14521 ggaatttttt tgttcggaga acgaagataa ggcaattcaa tcaaaacagg agaaaaacat
    14581 ggctgattgg gtaacaggca aagtcactaa agtgcagaac tggaccgacg ccctgtttag
    14641 tctcaccgtt cacgcccctg tgcatccgtt taccgccggg caatttacca agcttggcct
    14701 tgaaatcgac ggcgaacgcg tccaacgcgc ctactcctat gttaactcgc ccgataatcc
    14761 cgatctggag ttttacctgg tcaccgtccc cgatggcaaa ttaagcccac gattggcggc
    14821 actgaaaccg ggcgatgaag tgcaggtggt tagcgaagca gctggcttct ttgttctgga
    14881 tgaagtacca gattgcgaaa cgctatggat gctggcaacc ggtacagcga ttggccctta
    14941 tttatcgatt ctgcaactag gtaaagattt agatcgcttc aaaaatctgg tcctggttca
    15001 cgccgcacgt tatgccgccg acttaagcta tttgccactg atgcaagaac tggaaaaacg
    15061 ctacgaaggc aaactgcgca ttcagacggt ggtcagtcgg gaaacggcag cggggtcgct
    15121 caccggacgg ataccggcat taattgaaag tggtgaactg gaaagcgcga ttggcctgcc
    15181 gatgaataaa gaaaccagcc atgtgatgct gtgcggcaat ccacagatgg tgcgcgatac
    15241 gcaacagttg ctgaaagaga cccggcagat gacgaaacat ttacgtcgcc gaccgggtca
    15301 catgacagcg gagcattact ggtaagcggt tacttatcga taaacggcac gatgagcaaa
    15361 tccgcactca acttattgat catcccgcga tatgccggca tcaaacggtt gataaatgag
    15421 tggtgatgac cacagacaag gaggtcgcac tgctcttttt gcataatttc cagcagtgtt
    15481 tccggcattt ctccgcgttc aatacgcagt tttgtcttcg gccattgaat atttttcgtc
    15541 agtttataca gcttgttatc cgacttattc ttcaacaatt gaagaatatc ttctgttgca
    15601 gggaagtaga tacccggata caactcgctt aagccatcat caatatgaat taacgtcagg
    15661 tgagcgtcat tatgtctggc gagctccaag gctttattca ccagtaaggc atcttcttca
    15721 ttcccggaaa ttgccacgcc aatgtgttta taagccatgt ttaactcctt ctaaagcaac
    15781 tccatcaagc taacgaacgc agtgataact caaaaataat catctcggcc tggcatgaga
    15841 aggtgaaggc cgcatcaagc tcaacttgcc cctccagatc cttaatctta taggccacgc
    15901 tggccccttc atctgcagag gatgcagcgg cggcaagctc tttcagtttg gtcataaagg
    15961 actctgcttc ttcgcgggtg gcaaaaaagc gcgaaaactt actggtacag ttgtcgttat
    16021 caatcaccgt accgatatca atcgcacatc ctttagtact gcatttatct acgacatctt
    16081 tcatagggca cctctgtata ttcgcctctc tgtcgattac gacttaagaa taatgcgccg
    16141 ttaagggaag aacaaacaag taatgatctt atggattacc attttttttg atattgcgta
    16201 aggtgaagag ttcagaagga ttttccgggt attgtcattt gcatcgtgga aataacaatc
    16261 ccggaaaaaa atagcagtaa gagatatatt aaaggttaat tacttgattt attgtcggct
    16321 ttatacttca catcctgagt atctttacca tatttatttt cgccttgtgt gccaacaaac
    16381 gcaccaagat cgataagcat catcaccaga atcaacgtcg ggacaaaacg ccctaccgcc
    16441 cattgccaga cacccggtaa aatcgcccag ttacccgcca gtagcatcca cgccacaatc
    16501 atcagaaatg cccatgcgcc ggaacgcccg cgatcatgca agcgcttaac ggttactgcc
    16561 gctgttggcc agagcaagca cacaaggcaa aacgccgcgg tctgaatatc gagtaaattt
    16621 ttacccgcca gtgagaaaag caccagcatg cctgcgaacc acaggcctat ccaaatccag
    16681 aaatcacggc gtccaatacg ccctttaaat gagaataacc attgctgtat ggtcatgtaa
    16741 gttccttgat ggttgtcttt tccaggattc tacccttttg acaagggcga cagatatcgt
    16801 tttaatcgga gccagtcata acaaaaggta ctgtcaatga agccagggtg tacgctgttt
    16861 tttctcttat gttctgcatt aaccgttaca acaacggcgc atgcacaaac accagatacg
    16921 gcaacgaccg cgccttatct gctggctgga gcccctactt tcgatctctc catcagccag
    16981 tttcgagaag actttaacag ccagaatccc agcctgccac tgaacgaatt tcgtgccatc
    17041 gacagcagtc ccgacaaagc caatctcact cgtgctgcca gtaaaattaa cgagaacttg
    17101 tatgcttcta cagcgctgga gcgcggtacc ttaaaaatca aaagcattca aatgacatgg
    17161 ctacccatcc aggggccaga gcaaaaagcc gcgaaagcga aagctcagga atacatggca
    17221 gcggtgatcc gcacactcac cccattaatg accaaaacac aaagccagaa aaaactgcag
    17281 tcgctactaa cggcggggaa aaacaaacgt tattacaccg agacagaagg tgcactgcgc
    17341 tatgttgtcg cggacaacgg cgaaaagggg ctgaccttcg ctgttgaacc gattaagctg
    17401 gcgctatctg aatcgcttga aggtttgaat aaatgacaaa aagcaaagcc tttgtgccga
    17461 tgaatctcta tactgtttca cagacctgct gccctgcggg gcggccatct tcctttattc
    17521 gcttataagc gtggagaatt aaaatgcgac atcctttagt gatgggtaac tggaaactga
    17581 acggcagccg ccacatggtt cacgagctgg tttctaacct gcgtaaagag ctggcaggtg
    17641 ttgctggctg tgcggttgca atcgcaccac cggaaatgta catcgatatg gcgaagcgtg
    17701 aagctgaagg cagccacatc atgctgggtg cgcaaaacgt ggacctgaac ctgtccggcg
    17761 cattcaccgg tgaaacctct gctgctatgc tgaaagacat cggcgcacag tacatcatca
    17821 tcggtcactc tgaacgtcgt acttaccaca aagaatctga cgaactgatc gcgaaaaaat
    17881 tcgcggtgct gaaagagcag ggcctgactc cggttctgtg catcggtgaa accgaagctg
    17941 aaaacgaagc gggcaaaact gaagaagttt gcgcacgtca gatcgacgcg gtactgaaaa
    18001 ctcagggtgc tgcggcattc gaaggtgcgg ttatcgctta cgaacctgta tgggcaatcg
    18061 gtactggcaa atctgcaact ccggctcagg cacaggctgt tcacaaattc attcgtgacc
    18121 acatcgctaa agttgacgct aacatcgctg aacaagtgat cattcagtac ggcggctctg
    18181 taaacgcgtc taacgctgca gaactgtttg ctcagccaga catcgacggc gcgctggttg
    18241 gcggtgcgtc tctgaaagct gacgctttcg cagtaatcgt taaagctgca gaagcggcta
    18301 aacaggctta agtctgacag gtgccggatt tcatatccgg cacttacttt ccttaactct
    18361 tcgccttaac gcaaaatctc acactgatga tcctgaattt cctcggctga agcacggtta
    18421 agcgtcagta gatttcgttg tgtcgccagc aatacaaatg agttatcact ctgccgtacc
    18481 atcgccagcc cgtagcttcc catatgttcc cgcgcctcag gtacttcttc tgccagcatc
    18541 ataaatgggc tgcgttgtac cagttcgctt tccgttaccc gacgcgccag gtattcatgc
    18601 ccgcgcaaac cacctggcag tggcaaccag cggctgctga tgttcgccag attgttatcc
    18661 agctgttcgc gcacatcagg acgaatacaa gagatatgaa tatgaaaatg gttttgcgta
    18721 cgcccggtgc gggagttgat cgccaaagaa accgcgcgat cgggaaccgg ctggccgtat
    18781 tttttgctca taaaatcacg cgcctgccag gccaaccaaa agaagttcgg cgttgaagga
    18841 tcggtcaaca aaggactttc agtaccgtta atacgatacg ttggcatcaa cagatattgc
    18901 agtgggccat taagatcttt taaaaccacg tatccggcat tgggtttgac ttccgcacat
    18961 ggcgaaggat tttgattttg ctgctgattg ggcaaacatt cctcaaggac aatcttacgt
    19021 aatgtatccg actcttcacc ggttaatttc cagtaaccaa taccggcagc cacaacggcg
    19081 ataactatca tcaccaaaaa aagaagaccc gcttttttca tctttttttc cctgtacctc
    19141 aaagagatgc aagggtaacg caaaatcgtg acaaataaaa aacccggtcg cgagaaacca
    19201 ccgggtttga taattatcct ggggaaatca gcgtttgctg atctgatcga acgtaccgcc
    19261 gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg aactcttcat caatggtgaa
    19321 taacttcagc tttggaaacg cattttcgta ctttttcgcc acctcagcgt cgcgcggacg
    19381 gtagtagttt ttcgcggcaa tttcctgacc ttctggcgag tagagatatt tcaggtaggc
    19441 ttccgccacc tctttagtac cttttttctc gaccacttta tcgaccaccg acacggttgg
    19501 ctctgcgagg atagactcac tcggcgtgac gatttcgaat ttatctttcc ccagttcatt
    19561 cgctgccagc agagcttcgt tttcccagga aatcagtaca tcgccaattc cgcgctcgac
    19621 aaaagtgtta gtggagccgc gcgcgccaga atccagaact tcgacgtttt tatacagtgc
    19681 ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg tgatgcagcg cgtagcccca
    19741 ggctgccagg tagttccagc gcgcgccacc agagcttttc ggattaggcg tgatcaccga
    19801 aacacccagt ttaatcagat cgttccagtc atggatctgc ttcggatttc ccttacgtac
    19861 caggaaaaca atggtggaag tgtacggtgc ggagttatcc ggcagacgtt tgatccactc
    19921 tttatcaatc cgcccgcgtt ccgcaatcgc gtccacgtca taggccagag ccagcgtgac
    19981 aacatcagct tcaataccgt tgattaccga cgttgcttgt ttgcctgaac caccgtgtga
    20041 ctgacgaatc accacgttat cgccagtttg ctgtttccag tgggcgctga atgccttgtt
    20101 gtactgttcg tacaattcgc gcgttggatc atatgaaacg ttaagaagct gaatatcctt
    20161 tgccataacg ctggttgccg ccagcaaaaa tgttaaccct acgccccact tgttcatcgc
    20221 ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc agaaggttaa ccaaacatta
    20281 aagaataaaa aaagattggc tataacttgc gggtatatgt tgggggttaa aaaggcggaa
    20341 gaaatccgcc tcatattgct gacaaagtgc gctttatcga tgccggatgc ggcgcgaacg
    20401 ccttatccgg cctacaaaag tttgtaaatt caataaattg tagaattcac gtaggcctga
    20461 taagcgaagc gcatcaggca tttttgcttc tgtcatcggt ttcaggctaa aggaatctgc
    20521 ctttttccga aatcattagt acagtttttt cgcgcagtcc agccagtcgc ctttgaacgg
    20581 acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga accagctgtt cgttctgtat
    20641 acctacgcaa cgaccgccgt aacctgccag cagcagatcg atagcgtaag cgcccatacg
    20701 ggaagccaga atacggtcgt aaggcaccgg agaaccaccg cgctggatgt ggcccagcac
    20761 agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc
    20821 acacatatgt tcggtaatcg ccacgatcgc gtgtttttta cctttcgcga tacccgcttt
    20881 gatttcgttt accaggtctt cacggctgaa ttcaacttcc ggaaccacaa cgaattcaca
    20941 gccaccggca atggccgcag ccagcgtcag atcgccacaa taacggccca tcacttccac
    21001 cacggaaata cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg cttctacaac
    21061 ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat
    21121 agtgcccggc aggccgatgc acgggaagcc catttcggtc agacgcattg cacccatgta
    21181 ggaaccgtca ccgccgataa ccaccagcgc atcgatccca cgttttttca ggttttcgat
    21241 agccacggcg cggatgttct cgtcgcggaa ttccgggaaa cgcgcagaac cgaggaacgt
    21301 accgccacgg ttgatcatgt cagaaacgct gtaacggtct agctgtacca tacggtcttc
    21361 atacagaccc agatagccgt cataaatgcc cattacttcc agaccttctg tcagcgcaga
    21421 acgaacaacc ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac
    21481 accgattttc ttaatcatga ctacctctga actttggaat gcaaaataaa atctgttgcc
    21541 ggaagtcttc ttgcacatcg aagtgatcca acgaatgtgc aaatagtata acaatcactt
    21601 cctgctgaat tgattcaggt caggccaaat ggcggtattt tatacacaaa atgcgggtct
    21661 ggctctcttt tatactgatt atgaaagcat agaccgttta ccctccctag gtacgacgga
    21721 acaggggtcc tgatggataa tcacatccga tcccggaaaa cgccgtaaaa tagcctgctc
    21781 tacctgatcc gccaccatat gtgcctgaac caaaggcaga gagtcttcca tttccaaatg
    21841 aatctgaata aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag cgccgctaac
    21901 acccggccag gaagtcacga tatcaataat ttcttgccgt tcctcatcag gtaatgcgcg
    21961 atccagtaat gactgtaccg cctcatatcc catgcgtaac gcgctatata aaatatagat
    22021 gccgattccc aatgcaaaca gagcatcggc gcgatgccag ccgtaccagg acaaccccag
    22081 cgccagcaga attgcgccgt tcatcataac atcagactgg taatgtagca tatcagcccg
    22141 caccgcctgg ctttgcgtcc ggcgcaccac ccaacgctga aacgagacaa ggataatcgt
    22201 acaaattagc gccacaattg tcacgataac cccgacgcct ggatctgtca tcggtgttgg
    22261 agatatcaga tgttgaatgc ctgtcaaaaa caggaatagt gccgaaccgg agataaacat
    22321 actttgcgcc agcgccgcga gggactcagc tttaccgtga ccaaacgagt gattatcgtc
    22381 ggcaggttgc agggaatatc gcaccaccaa taaattcgtc aacgacgcgc cgatatccac
    22441 cagcgaatcc accagcgcgg cgagaatact caccgacccg gtataccacc atgcaaaaat
    22501 tttaatcagc aatagcagtg aagccatcgc cgtcgcagca atcgccgccc gactgaccag
    22561 ccgtccataa gattgattca taaatactcc cgctatcaac tgacgctagt ataacggaag
    22621 caaatcatct gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa cccccacatc
    22681 atgtggggga agacagggat ggtgtctatg gcaaggaaaa cagggtttac tactgggaac
    22741 gtgagttgct actactcaat agcttcaacg atgaactttt ttgccattgc gtcacgtcac
    22801 gcaactgctc cattcgttgt tgatgtttct cgtttaaaac cgcttgctgc tccggcgtta
    22861 acaggcgata catttggttg cggacttttg ccatctcaac ctgacgagca atttgctcat
    22921 tcgccatttt ttctgcctgt gcgcgcacag cgttttcatc aaaattttct gcggtgacaa
    22981 gacgatgcat tgtctccagt tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt
    23041 gcataagatc tcgcatctgc tgacgctgat gttcggttaa acttatgccg tcgaacatat
    23101 ggctctgcgt actgcgctgc gtaagttctt cacccggatg ccagttatcg cctgaaccga
    23161 cttcagcagc gtggcttaat gaactgactg ccagcgttga ggccatgacg gcagcggtaa
    23221 ctatgcgcat catttgctcc caaaatcttt ctgtcgcgat tcaacgatag agagtttacg
    23281 attcaggctg caaacatgcg tcagggggtg taaaacaacg taaagtcatg gattagcgac
    23341 gtctgatgac gtaatttctg cctcggaggt atttaaacaa tgaataaaat cctgttagtt
    23401 gatgatgacc gagagctgac ttccctatta aaggagctgc tcgagatgga aggcttcaac
    23461 gtgattgttg cccacgatgg ggaacaggcg cttgatcttc tggacgacag cattgattta
    23521 cttttgcttg acgtaatgat gccgaagaaa aatggtatcg acacattaaa agcacttcgc
    23581 cagacacacc agacgcctgt cattatgttg acggcgcgcg gcagtgaact tgatcgcgtt
    23641 ctcggccttg agctgggcgc agatgactat ctcccgaaac cgtttaatga tcgtgagctg
    23701 gtggcacgta ttcgcgcgat cctgcgccgt tcgcactgga gcgagcaaca gcaaaacaac
    23761 gacaacggtt caccgacact ggaagttgat gccttagtac tgaatccagg ccgtcaggaa
    23821 gccagcttcg acgggcaaac gctggagtta accggtactg agtttaccct gctctatttg
    23881 ctggcacagc atctgggtca ggtggtttcc cgtgaacatt taagccagga agtgctgggc
    23941 aaacgcctga cgcctttcga ccgcgctatc gatatgcaca tttccaacct gcgtcgtaaa
    24001 ctgccggatc gtaaagatgg tcacccgtgg tttaaaacct tgcgtggtcg cggctacctg
    24061 atggtttctg cttcatgata ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga
    24121 cgctggcgct ggtgttgatg ttggttttga tgttacccaa gctcgattca cgccagatga
    24181 ccgagcttct ggatagcgaa cagcgtcagg gtctgatgat tgagcagcat gttgaagcgg
    24241 agctggcgaa cgatccgccc aacgatttaa tgtggtggcg gcgtctgttc cgggcgattg
    24301 ataagtgggc accgccagga cagcgtttgt tattggtgac caccgaaggc cgcgtgatcg
    24361 gcgctgaacg cagcgaaatg cagatcattc gtaactttat tggtcaggcc gataacgccg
    24421 atcatccgca gaagaaaaag tatggccgcg tggaactggt cggtccgttc tccgtgcgtg
    24481 atggcgaaga taattaccaa ctttatctga ttcgtccggc cagcagttct caatccgatt
    24541 tcattaactt actgtttgac cgcccgctat tactgctgat tgtcaccatg ttggtcagta
    24601 cgccgctgct gttgtggttg gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg
    24661 ctgccgatga agttgcccag ggaaacttac gccagcaccc ggaactggaa gcggggccac
    24721 aggaattcct tgccgcaggt gccagtttta accagatggt caccgcgctg gagcgcatga
    24781 tgacctctca gcagcgtctg ctttctgata tctctcacga gctgcgcacc ccgctgacgc
    24841 gtctgcaact gggtacggcg ttactgcgcc gtcgtagtgg tgaaagcaag gaactggagc
    24901 gtattgaaac cgaagcgcaa cgtctggaca gcatgattaa cgacctgttg gtgatgtcac
    24961 gtaatcagca aaaaaacgcg ctggttagcg agaccatcaa agccaatcag ttgtggagtg
    25021 aagtgctgga taacgcggcg ttcgaagccg agcaaatggg caagtcgttg acagttaact
    25081 tcccgcctgg gccgtggccg ctgtacggca acccgaacgc cctggagagt gcgctggaaa
    25141 acattgttcg taatgccctg cgttattccc atacgaagat tgaagtgggc tttgcggtag
    25201 ataaagacgg tatcaccatt acggtggacg acgatggtcc tggcgttagc ccggaagatc
    25261 gcgaacagat tttccgtccg ttctatcgga ccgatgaagc gcgcgatcgt gaatctggcg
    25321 gtacaggttt gggactggcg attgttgaaa ccgccattca gcagcatcgt ggctgggtga
    25381 aagcagaaga cagcccgctg ggcggtttac ggctggtgat ttggttgccg ctgtataagc
    25441 ggagttaaac tccgcatttg taggcaggat aaggcgttta cgccgcatcc ggcatttgag
    25501 caggatgcct gatgcgacgc tgatagcgtc ttatcaggcc tacactccgg gggttatttt
    25561 ccccacaacc ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt ttgcatcgtt
    25621 cgcgtccagc ttttcgataa cccggcagcg gagagtaaac ggtgatactg atcgtcatca
    25681 aacggcatat gccatgcgat ggcagcagcc tcctgcacgg tcacatcgct gacacgggaa
    25741 accaactcca gcggcgcgtc cgcagacact ttccctggcg ctatcacgct atacaaccat
    25801 cccactttac ccgtgttttg catcaactgc gctatatcgc tgatatcaaa atggtaattg
    25861 agtttatagc aaggcgagcg cggctggctg acctgaatta atgcctctcc ccagcggaaa
    25921 atatcgccaa tataaacatt actttccgtg agaccgtcgg ttgagagatt ttcgccaaac
    25981 gcaggcgcaa caaacaactc cgcctgttcc ggaaattccc gcgcccagta gagataatgc
    26041 tcacgaggat aatgacacag cgctctgtct ggtccgccgt gaaccttttt ctccgcctgc
    26101 tcgtcacctt ccagccccag ctccgttagc atcaactcac catcaacctg gattttggca
    26161 attgcgctgg gtttgccttc gggataagcc tgaatcttgc ctgtgtatac atcaaccgga
    26221 tatcgcatct gttccctctc cacgagaaat ttccccatta gaccatattt attttatgtc
    26281 aggcgtgctg ttttcgcggt aagtatccag gcaggaactc aatagcaaac cggacgaacg
    26341 ttagttcatc cggctgtggg gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc
    26401 gattttcgcc gctctggctt tgactttacg tgaccagata gaggtaagaa ttggcaccag
    26461 aatcgaagtc acaatgactg ccgtcgctac cagcgaagtt gctgccggag ccatcggttt
    26521 aaacgcaggc accatttctg caatcagcac aggtgtcgct accgcggccc ctgcggaact
    26581 ggaagcggca attccggcag tgccatcgcc accgccaatc aatttatcgg cgataatcaa
    26641 cggaataccg gtcacgataa ttactgccac acccaacagg atccccagca aacctgtctg
    26701 ggcaattaca gtcaaatcaa tggtattgcc tagtgcgaag gcaaagaatg gaatcagcgt
    26761 ctgcaccgct ttgctgaaaa attctcgtaa ttcagggtca aggttcccaa gggcaaagcc
    26821 caccaggaac ggtaatactg cgccgacgaa aacatgcggt tcaaacgagg caatcccggc
    26881 agtgcccaga ataatcatcg tcatgagcgg cccggactcc aacgacatca acacaaatgc
    26941 cccagcttct tcttttgtgc cgtactgctg catgatggaa gcgtaaagtc cgccgttggt
    27001 catatccatc gccgccacca gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac
    27061 accatgttcc ggaataatgc gcgaggcaat tgccgcaacc acccacgcga cggcaatttt
    27121 agttaccacc agcgtaccgg atttacgcag taccgttccc gtcgcgctta attttattga
    27181 cgcccccatg caaaaaaacc acaccgccag aatgggcacc gtaccggtaa tcatcccgtt
    27241 ggtgaatgat ccaaaatatt tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag
    27301 gaatagcggg acgagcatca tcccccccgg gattttctca atcaagcgtt ttatctgcat
    27361 ttccatcacc tgtcggcata tttttaaggg ctgatgcgta attcctcaat ggagcatcag
    27421 tgttgatgga aacgaagata gacattcatt taatcaataa aagtgatcaa aaccacatca
    27481 actgaaacgc tgttttattt ttatcggatc attgttttta ttttgaaatt gatcacaaaa
    27541 aaaccaccgc cattggcgat ggttcatttt ttaagctgat atgcggcgta aacgcctcac
    27601 tgcagcagac ggcaaatgat tattttttcg ccgcgaaacg tgccgctgct tcgtcccagt
    27661 tcaccacgtt ccagaactct ttgatgtagt ccgggcggcg gttctggaat ttcaggtagt
    27721 aagcatgctc ccacacatcc aggcccagaa tcgggaagcc ggatgcgcca gaaatagctt
    27781 cacccatcag cggggagtct tggttagcag tagaaaccac cgccagttta tcgcctttca
    27841 gcaccagcca tgcccagccg gaaccaaagc gggaagctgc cgctttctca aattctgctt
    27901 tgaagttatc tacggaaccg aaatcacgtt cgatagccgc tttcaggtca ccctgcaggg
    27961 tggtgccttt tttcagacct ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt
    28021 tgcgcagtac ggttttcttg tctgctggca gctggtccag tttggtgatc aactcttcaa
    28081 ccggcaggtt ggcaaattct ggcaggcttt ccaacgccgc gttggcgttg ttgacgtagg
    28141 tctgatggtg tttggtgtgg tggatttcca tggtctgctt atcgaagtgc ggttccaggg
    28201 catcgtaagc atacggcagg gatggcaggg tatagctcat attcatctcc agtattgtcg
    28261 ggcggccgat tgttaatgcc gcgtaagcag ttggttcatt atagttaatt aaatgatatt
    28321 gaaaatgatt atcaatgccg tacttttcgt aagggtatgg ttttgcagga aaatgcccga
    28381 gatgtgaagc aaatcaccca cttaatgccg tgattgccag taaatcgaca acggcggcaa
    28441 caggcgaaag gttaatcgac agcacgattt ttacactcat ctcgtcggag atgtgacgcg
    28501 acgaaaaatg atgaggataa gaagatgagt aacgcgatta cgatggggat attttggcat
    28561 ttgatcggcg cggccagtgc agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg
    28621 tcatgggaaa ccatgtggtc agtcggtggg attgtttcgt ggattattct gccgtaggcc
    28681 atcagcgccc tgttactacc gaatttctgg gcgtattaca gctcgtttag tctctctacg
    28741 ctgctgcctg tttttctgtt cggcgctatg tgggggatcg gtaatatcaa ctacggcctg
    28801 accatgcgtt atctcggcat gtcgatggga attggcatcg ccattggcat tacgttgatt
    28861 gtcggtacgc tgatgacgcc aattatcaac ggcaatttcg atgtgttgat taacaccgaa
    28921 ggcggacgca tgacgttgct cggcgttctg gtggcgctga ttggcgtagg gattgtaact
    28981 cgagccgggc agttgaaaga gcgcaagatg ggcattaaag ccgaagagtt caatctgaaa
    29041 aaaggactgg tgctggcggt gatgtgcggc attttctctg ccgggatgtc ctttgcgatg
    29101 aacgccgcaa aaccgatgca tgaagccgct gccgcactag gcgtcgatcc actgtatgtc
    29161 gctctgccaa gctatgttgt catcatgggc ggcggcgcga tcatcaacct cggtttctgt
    29221 tttattcgtc tggcaaaagt gaaggattta tcgctaaaag ccgacttctc gctggcaaaa
    29281 ccgctgatca ttcacaatgt gttactctca gcactgggcg ggttgatgtg gtatctgcaa
    29341 ttctttttct atgcctgggg ccacgcccgc attccggcgc agtatgacta catcagttgg
    29401 atgctgcata tgagtttcta tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag
    29461 tggaacaatg caggacgccg tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt
    29521 gttgccgcca acatcgttgg catgggcatg gcgaattaat ctttctgcga attgagatga
    29581 cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta
    29641 tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg ctggagaaga
    29701 tattgcgcat gacacactct gacctgccgc agatattgat tgatggtcat tccagtctgc
    29761 tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaatttatcc
    29821 agcgcaaagg gacttttcag gctagccgcc aaccgggtaa tcagcttatc cagcaacgtt
    29881 tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc
    29941 aactgcccga acagcaactc tgccatttcg ttagcaaacg gcacatgctg actactttca
    30001 tgctcaagct gaccgataac ctgccgcgcc tgtgccatcc ccatgctacc taagcgccag
    30061 tgtggttgcc ctgcgctggc gctaaatccc ggaatcgccc cctgccagtc aagattcagc
    30121 ttcagccgct ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag
    30181 gagtgtttat cgtcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga
    30241 tcgttgagta catgcagacc attaccgcgc cagacaatca ccagctcaca aaaatcatgt
    30301 gtatgttcag caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg
    30361 ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc
    30421 agagaacgaa attgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta
    30481 ttgcagaaag ccatcccgtc cctgacgaat atcacgcggt gaccagttaa actctcggcg
    30541 aaaaagcgtc gaaaagtggt tactgtcgct gaatccgcaa tgataggcga tgtcagtaac
    30601 gctggcctcg ctgtgacgta gcagatgtcg ggctttcatc agtcgcaggc gattcaggta
    30661 tcgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac gcagtgaaag
    30721 agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca
    30781 ggccagaagc aagttgagac gtgatgcgct gttttccagg ttctcctgca aactgctttt
    30841 acgcagcaag agcagtaatt gcataaacaa gatctcgcga ctggcggtcg agggtaaatc
    30901 attttcccct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaatacgct
    30961 gtggttaacg cgccagtgag acggatactg cccatccagc tcttgtggca gcaactgatt
    31021 cagtccggcg agaaactgaa atcgatccgg cgagcgatac agcacattag tcagacacag
    31081 attatcggta tgttcataca gatgccgatc atggtcgcgc acaaaacaga ccgtgccacc
    31141 ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac
    31201 aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat
    31261 cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc
    31321 ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt
    31381 ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga
    31441 aatgcggtga gcatcacatc accacaattc agcaaattgt gaacatcatc acgttcatct
    31501 ttccctggtt gccaatggcc cattttcctg tcagtaacga gaaggtcgcg aattcaggcg
    31561 ctttttagac tggtcgtaat gaaattcagc aggatcacat tatgaccttt cgcaattgtg
    31621 tcgccgtcga tctcggcgca tccagtgggc gcgtgatgct ggcgcgttac gagcgtgaat
    31681 gccgcagcct gacgctgcgc gaaatccatc gttttaacaa tgggctgcat agtcagaacg
    31741 gctatgtcac ctgggatgtg gatagccttg aaagtgccat tcgccttgga ttaaacaagg
    31801 tgtgcgagga agggattcgt atcgatagca ttgggattga tacctggggc gtggactttg
    31861 tgctgctcga ccaacagggt cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgca
    31921 ccaatggcct aatggcgcag gcacaacaac aactcggcaa acgcgatatt tatcaacgta
    31981 gcggcatcca gtttctgccc ttcaatacgc tttatcagtt gcgtgcgctg acggagcaac
    32041 aacctgaact tattccacac attgctcacg ctctgctgat gccggattac ttcagttatc
    32101 gcctgaccgg caagatgaac tgggaatata ccaacgccac gaccacgcaa ctggtcaata
    32161 tcaatagcga cgactgggac gagtcgctac tggcgtggag cggggccaac aaagcctggt
    32221 ttggtcgccc gacgcatccg ggtaatgtca taggtcactg gatttgcccg cagggtaatg
    32281 agattccagt ggtcgccgtt gccagccatg ataccgccag cgcggttatc gcctcgccgt
    32341 taaacggttc acgtgctgct tatctctctt ctggcacctg gtcattgatg ggcttcgaaa
    32401 gccagacgcc atttaccaat gacacggcac tggcagccaa catcaccaat gaaggcgggg
    32461 cggaaggtcg ctatcgggtg ctgaaaaata ttatgggctt atggctgctt cagcgagtgc
    32521 ttcaggagcg gcaaatcaac gatcttccgg cgcttatctc cgcgacacag gcacttccgg
    32581 cttgccgctt cattatcaat cccaatgacg atcgctttat taatcctgag acgatgtgca
    32641 gcgaaattca ggctgcgtgt cgggaaacgg cgcaaccgat cccggaaagt gatgctgaac
    32701 tggcgcgctg cattttcgac agtctggcgc tgctgtatgc cgatgtgttg catgagctgg
    32761 cgcagctgcg cggtgaagat ttctcgcaac tgcatattgt cggcggaggc tgccagaaca
    32821 cgctgctcaa ccagctatgc gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg
    32881 aagcctcgac gctcggcaat atcggcatcc agttaatgac gctggatgaa ctcaacaatg
    32941 tggatgattt ccgtcaggtc gtcagcacca ccgcgaatct gaccaccttt acccctaatc
    33001 ctgacagtga aattgcccac tatgtggcgc ggattcactc tacacgacag acaaaggagc
    33061 tttgcgcatg accactcaac tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc
    33121 agtagggatt gatgtcgagg aggcgctgcg ccaacttgat cgtttacccg tttcaatgca
    33181 ctgctggcag ggtgatgatg tttccggttt tgaaaacccg gaaggttcgc tgaccggagg
    33241 gattcaggct actggcaatt atccgggcaa agcgcgtaat gccagtgagc tacgtgccga
    33301 tctggaacag gctatgcggc tgattccggg gccaaaacgg cttaatttac atgccatcta
    33361 tctggaatca gacacgccag tctcgcgcga ccagatcaaa cctgagcact tcaaaaactg
    33421 ggttgaatgg gcgaaagcca atcagctcgg tctggatttt aacccctcct gtttctcgca
    33481 tccgctaagc gccgatggct ttacgctttc ccatgccgac gacagcattc gccagttctg
    33541 gattgatcac tgcaaggcca gccgccgcgt ttcggcctat tttggtgagc aactcggcac
    33601 accgtcggtg atgaacatct ggatcccgga tggcatgaaa gatatcaccg ttgaccgtct
    33661 cgctccgcgc cagcgtctgc tggcagctct ggatgaggtg atcagcgaga agctggatcc
    33721 ggcgcaccat atcgacgccg ttgagagcaa attgtttggc attggcgcag agagctacac
    33781 ggttggctcc aatgagtttt acatggggta tgccaccagc cgccagactg cgctgtgcct
    33841 ggacgccggg catttccacc cgactgaagt gatttccgac aagatttccg ccgccatgct
    33901 gtatgtgccg cagttgctgc tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt
    33961 agtgctgctg gatgatgaaa cccaggcgat tgccagtgag attgttcgtc acgatctgtt
    34021 tgaccgggtg catatcggcc tcgacttctt tgatgcctct atcaaccgta ttgctgcgtg
    34081 gatcattggt acacgcaata tgaaaaaagc cctgctgcgt gcgttgctgg aacctaccgc
    34141 tgagctgcgc aagctggaag cggcgggcga ttacactgcg cgtctggcac tgctggaaga
    34201 gcagaaatcg ttgccgtggc aggcggtctg ggaaatgtat tgccaacgtc acgatacgcc
    34261 agcaggtagc gaatggctgg agagcgtgcg ggcatatgag aaagaaactt tgagtcgccg
    34321 cgggtaaaca ctgccggatg cggcacaagt gccttatccg gcctacaggc cggcaatagt
    34381 tgtaggcctg ataagacgcg acagcgtcgc atcaggcatt gattgccgga tgcggcacga
    34441 gcgccttatc cggcctacgg gtcgtgcatc tgacaacacc gaatttacag gaacacagaa
    34501 catgcaaaac attacccagt cctggtttgt ccagggaatg atcaaagcca ccaccgatgc
    34561 ctggctgaaa ggctgggacg agcgcaacgg cggcaacctg acgctgcgcc tggatgacgc
    34621 cgatatcgcg ctttatcacg acaacttcca cccacaaccg cgctatattc cgctcagcca
    34681 acccatgcct ttactggcaa atacgccgtt tattgtcacc ggctccggca aattcttccg
    34741 taacgtccag ctcgatcctg cagctaactt aggcgtcgta aaagtcgaca gcgatggcgc
    34801 gggctaccat attctctggg ggttaaccaa cgaagccgtt ccaacttctg aacttccggc
    34861 tcactttctc tcccactgcg agcgcattaa agccaccaac ggtaaagatc gggtgatcat
    34921 gcactgccac gccaccaacc tgatcgccct cacctatgta cttgaaaacg acaccgcggt
    34981 cttcactcgc caactgtggg aaggcagcac cgagtgtctg gtggtattcc cggatggcgt
    35041 tggcattctg ccgtggatgg tacccggcac ggacgaaatc ggccaggcaa ctgcccaaga
    35101 gatgcaaaaa cattcgctgg tgttgtggcc cttccacggt gtgttcggtt ccggaccgac
    35161 gctggatgaa accttcggtt taatcgacac cgcagaaaaa tcagcggaag tgttagtgaa
    35221 ggtgtattcg atgggcggca tgaaacagac tatcagccgt gaagagttga tagcgctcgg
    35281 ccagcgtttc ggcgttacac cactcgccag tgcgctggcg ctgtaaggag caaacatgat
    35341 ccgcaaagcc tttgtcatgc aggtaaaccc cgacgcccac gaagagtatc agcgtcggca
    35401 taatcccatc tggccggaac tggaagcagt gttgaaatct cacggtgcgc ataactacgc
    35461 catttacctc gacaaagcgc gtaatctgct gtttgccacg gtagagattg aatctgaaga
    35521 acggtggaat gcggtagcca gcactgaaat ctgccaacgt tggtggaaat atatgaccga
    35581 tgttatgccc gctaacccgg ataacagccc ggtgagtagc gagctgcaag aagtgtttta
    35641 cctgccttaa tgagttgcgg tgccggaaaa tatccagcac cgcgacttca tcagaagaag
    35701 tatttaaagc gaacgcgtgc gccgtaacgg tcatcagtat catcaccgtg ttttttatcg
    35761 ccgtcgctat ccagaatgga gtaatacgtc ccgaggtaaa tattaaagtt ctccatatcc
    35821 atcacgttag cgaactgata agacgcatga atggtgtgaa tggtgtacgt cccttcatca
    35881 tcaatgcagc agtcgttatc acaaactaca ccgctgaatt catcaatctt attgtgcgcg
    35941 tagatataac cgagctcgaa acgtttccac agcgcgttaa tcccggcagt gaaatctttc
    36001 tcattattag catctaaata ggcggtattt agattaacca caatgccatt gtccggatcc
    36061 gttttcaggc cgttccaggt catgctcata ccatagccgg tacgatcgga ctgatcgaca
    36121 aaattaccct tgctatcggt ataaccataa gcattattta ccacgttcgc ttccatcgct
    36181 gcggaaacgg tgaattcttc cgtcggcgac caggcaatta ctgggcgcag ataagcaaca
    36241 tttttctgct gctccatatc gcgtccatga taattaccgt cattatataa agatgttccg
    36301 tcttcaagta acgtgtttaa ttcaaaatac cagttatcga gttgtttgct gacgaggaaa
    36361 ttaccgccag cgttagagcg cccgcggccc tctttcatca tatagatata accgctgcca
    36421 tcgtcataaa gatcgttcgc agtattaccg gaatgttcaa caaaggtatc ctgattcaat
    36481 gggaacatat cgtaggcttc aaaacggcct actttgacct tccagtcgtt ttctttgcca
    36541 aagaagaata ccgcatcatc gatatttact gaaccgtgca tatcacccag cggttgtgcg
    36601 gagaacccgg cgaaataacc gttgtccatc tttcgcatac cgtcaaaacc taacagaata
    36661 cgaccattga gattccactg ttcattagtg ggatcgctat tcacatcagc gttggtcatt
    36721 gccagcaggc catgattact ttccgcatcc atattaaatt ctacgtcgcc gtagattttt
    36781 aaatcgccat aacctgaaag caaaagatta ggtgaggtgg catttttctt tgcagattcc
    36841 gccgttgcct gtccatttac cggcgtattg ccctgagcgg ccttaatttc gcggatctca
    36901 gcggcttgtt gtgtctgcag ctgtttaacc tggctctctg cttttgccgc ccgcatttct
    36961 gccgcttcca ggcgttcttc cagcaatgcc atgcgttgtt ccagcattaa atgctgtgtt
    37021 tttgccatac cggtactaca tgccaataat aaaccgatgg ctaaagataa tgtcgatttt
    37081 ttcattattt acgttccctg aaataaagga gaccctacgc tttccaaaag gaatacgtat
    37141 atagagaaat ataaggtgtg cactttcgaa tttttaatcc aacttcagca ggccgatgga
    37201 tagtatcccg ccgcataata tgagtaaatc cgctgatttc ctggaacgtg aggaa
//
LOCUS       DAZKIF010000033        36444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZKIF010000033 DAZKIF010000000
VERSION     DAZKIF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.033"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1280
                     /locus_tag="ACHOTW_001688"
     CDS             <1..1280
                     /locus_tag="ACHOTW_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFP9843816.1"
                     /translation="AIAAPLPQWANAPAVTPVAQLSLQESILRAFARNPGVTQQAAQI
                     GIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDSSGRLDNNVSYGITLTQLVYDFGKTNN
                     DINLQTAARDSYRFKLMATLTDVAEKTATAYMEVSRYQALCDAAQRNIHSLENVYNMA
                     ALRANAGLNSSSDELQAQTRIAGMRSTLEQYQAQMASAKAQLAVLTGVQPEAIAAPPA
                     ELAEQPVSLKNIDYQSIPLVLAAENLRQSAQYGVEKTKAQYWPTLSIQGGKTRYQTSD
                     RSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQQKISASQVEQAKLDVLQRASVAYANW
                     TGARGREEAGLAQSESAHKTRDVYQNEYKLGKRSLNDLLTVEQDVFQAQSAEINANYD
                     GWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDLK"
     gene            1383..5768
                     /locus_tag="ACHOTW_001689"
     CDS             1383..5768
                     /locus_tag="ACHOTW_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9843817.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            5820..21542
                     /locus_tag="ACHOTW_001690"
     CDS             5820..21542
                     /locus_tag="ACHOTW_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9843818.1"
                     /translation="MATDDIINASEKGADLQISGTSNQPAGTTITLALNGQNYTATTD
                     AAGNWSTTVPASAVGALGEASYTVTANVTDSAGNSNSASHNVQVNTALPGVTINPVAT
                     DDIINAAESGNAQTISGQVTGAAAGDTVTVTLGGKTYTATVQGNLSWSVDVPAADIQA
                     IGNGNLTVNASVTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVVNSIEHAQALVIT
                     GSSSGLAAGAALTVVINTVTYAATVLADGTWSVGVPAADVSNWPAGTVNITVSGTNTA
                     GTTSTITHPVTVDLAAVAISINTVSGDDVINAAEKGADLTLSGSTSGVEVGQTVTVTF
                     GGKTYTATVAGDGSWTTTVPAADLSVLRDGDATVQASVSTINGNTASATHAYSVDATA
                     PTLAINTIATDDILNAAEAGNPLTISGSSTAEAGQTVTVTLNGVTYSGSVQADGSWSV
                     SLPTADLSNLTASQYTVSASVSDKAGNPASANHGLAVDLTVPVLTINTVSGDDIINAA
                     EHGQALVISGSSTGGEAGDVITVTLNSKTYTTMLDASGNWSVGVPAADVTALGSGPQT
                     ITAAITDAAGNSDDASRTVTVNLAAPTIGINTIATDDVIKATEKGADLQITGTSNQPA
                     GTTITVTLNGQNYTATTDSNGNWSATVPASAVSALGEANYTVTANVTDTAGNSNSASH
                     NVLVNSALPAVTINAVATDDIINAAESGNAQTISGQVTGAAQGDTVTVTLGGNTYTAT
                     VQSNLSWSVDVPAADIQALGNGDLTVNASVTNGVGNTGSGSRDITIDANLPGLRVDTV
                     AGDDVINSIEHNQALVITGSSSGLTAGTALTVEINNVTYGATVLADGTWSLGVPAVDV
                     SNWPAGTVNITVSGTNSAGTTSTITHPVTVDLAGVAITINTLSGDDVINAVEKGETLV
                     VSGSTSGVEAGQTVTVTFGGKNYTTTVEANGSWTVNVPPADLAALPDGAGNVQASVSN
                     INGNSAQADRAYSVDATAPLVTINTIASDDILNVSEAGAGITISGTTTAQAGQTLTVT
                     LNNNTYQTTVLADGTWSVNVPAADLSGLTASSYTVTATVSDKAGNPASADHALVVDIT
                     APDLTINTVAGDDIINAIEHGQALVVSGTSTGAAAGDVVTVTLNGKNYTTTLDASGNW
                     SVGIPAADVTALATGSQTITASLSDRAGNSDSTTHDVTVDLSGPTLTINTVSGDDIIN
                     AAEIVVAQTISGQVTGTAVAGNTVIVTIGGNQYNATVQSDLSWSVSVPANVLQALGNG
                     ELTISASLTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIEHTQALVITGSSS
                     GLAAGAALTVVINSVTYGATVLADGSWSVGVPVADVTNWPAGTVNIAVSGTNTAGTTT
                     SISHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSGVEAGQTITVIFGGKS
                     YTTTVAADNTWGLTIPAVDVATLPDGAANVQASVSNVAGNSTQATHAYSVDATAPSVT
                     INTIATDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNVQADGSWSVSVPT
                     GDLASLTASSYTVNASVSDKARNSASATHNLTVDLAAPVVTINTVAGDDIINATEHGQ
                     AQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVISALAQGDVTITAT
                     VTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINAAEKGADLAITGTSNQPAGTQI
                     TVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDADGNSGSASHNVQV
                     NTALPGVTINVVATDDIINAAEAGVEQTISGQVTGAAAGDTVTVTLGGATYTATVQAN
                     LSWSVDVPASALQELGNGELTISASVTNSVGNTGNGTREITIDANLPGLRVDTVAGDD
                     VVNIIEHGQALVITGSSSGLAAGSNVTLTINGQTYVAAVLADGTWSVGVPAVDVSAWP
                     AGSVTIAASGSTSAGNPVSVTHPVTVDLSAVAVSINAITADDVINAAEKGAALTLSGS
                     TSGVEAGQTVTVTFGGKTYSATVAANGSWSTSVPAADMAALRDGDASAQASVSNVNGN
                     SATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTAEAGQTVTVTLNGT
                     NYTGTVQTDGSWSVSVPSADLSTLTASNYTVNAAVSDKAGNPASVNHNLTVDTSVPVV
                     TINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTTVLDASGNWSVGV
                     PASVVSALANGTVTINASVTDAGGNSGSATHQVTVNTGLPTITFNAISGDNILNADEK
                     GQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVSDLAALGQANYTVS
                     ASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGADQTISGVVTRAAAG
                     DTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASVTNANGNTGSGTRD
                     ITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVLTVTINSVAYSATV
                     QADGSWSVGIPAANVSAWPAGPLTVEVDGQSSANNPVSVSHPFTVDLTAVAISINTVA
                     SDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASLAANGSWSVNVPAADLA
                     TLPEGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDILNAAEAGSPLTI
                     SGTSTAETGQTVTVTLNGATYTGTVQADGSWSVSVPTSALGALNASNYTVSATVNDKA
                     GNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTSSGGEAGDVVSVVL
                     NGKTYTTTLDASGNWSVGVPAADVTALGSGAQTITASVSDRAGNSDDASRTVTVSLSA
                     PVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQNYSATTDASGNWSV
                     TVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGITINPVATDDIINAS
                     EAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVPAADWQALGNGELT
                     VNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHAQAQVITGSSSGFA
                     AGTALTVVINNQTYAATVLANGSWSVGVPATDVSNWPAGTLNITVSGANSAGTQTSIT
                     HPLTVDLTAVAISMNSITSDDAINAAEKGAALTLSGSTSGVEAGQTVTVTFGGKTYTT
                     TVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEYSVDSAAPTVTINT
                     IASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQTDGSWSLTLPASDL
                     TALANNGYTLTATVSDLAGNLGSASKGVTVDTTAPVISFNTVAGDDVINNVEHIQAQI
                     ISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGLADGTVTISATITD
                     SAGNSSTQTHNVQVNTAAVSLSVSTISGDNLINAAEAGSALTLSGTGTNFATGTVVTV
                     LLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSAGNSATASRSVAVD
                     LTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGGKTYTATVAANGT
                     WALNVPAVDLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAPTVTIATVAGDDII
                     NNAEQLAGQTISGTTTAEVGQTVTVTFNGQTWSATVGSGGSWSVFIPAQQFAGLSDGS
                     YTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAEHGSSLVISGTTTA
                     PVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVINASVSNAIGNTGS
                     SNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSLTAALASNETAQIS
                     IDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGATDSQNVVIDTTAP
                     DPAVKTIAISAITTDMGLITNDFVTSDTTLAVSGTLGATLSAGEFAQISLDGGVTWTT
                     LTVVGTSWSYADGHTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSITIT
                     GISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTVAADSLNW
                     SYVDGRTLTNGTTTWQVRVVDLAGNVGATSSQSALIDTVNPAQVLTIASISTDTGSSA
                     TDFITSDTMLTLTGSLGAGLASGEVAQISLDSGATWTTLTTNGTQWTYTDSRTLTDGS
                     YVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQATDDT
                     TPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSARVVD
                     LAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVVING
                     KTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNANISNG
                     TVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKTALT
                     LYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTVGTL
                     TWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATVGGA
                     VTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNTTNTFL
                     SGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGCPVAVGAT
                     ATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQSALGGSQSGTTSG
                     VAAMDYDWDGAVDVLVSKQSGSVFLSRNTNTVSYGTSLHLRITDPNGINVYYGNTVKL
                     YNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNIDQTV
                     NTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGWVYS
                     SGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSNFNDIL
                     TGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTWEGT
                     ADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQIDR
                     DGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            21546..23708
                     /locus_tag="ACHOTW_001691"
     CDS             21546..23708
                     /locus_tag="ACHOTW_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFP9843819.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            23705..24880
                     /locus_tag="ACHOTW_001692"
     CDS             23705..24880
                     /locus_tag="ACHOTW_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFP9843820.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            24877..25284
                     /gene="cueR"
                     /locus_tag="ACHOTW_001693"
     CDS             24877..25284
                     /gene="cueR"
                     /locus_tag="ACHOTW_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP9843821.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(25288..25491)
                     /locus_tag="ACHOTW_001694"
     CDS             complement(25288..25491)
                     /locus_tag="ACHOTW_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843822.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(25488..25910)
                     /locus_tag="ACHOTW_001695"
     CDS             complement(25488..25910)
                     /locus_tag="ACHOTW_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9843823.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(25995..27011)
                     /locus_tag="ACHOTW_001696"
     CDS             complement(25995..27011)
                     /locus_tag="ACHOTW_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9843824.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            27378..27740
                     /locus_tag="ACHOTW_001697"
     CDS             27378..27740
                     /locus_tag="ACHOTW_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843825.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(27919..28377)
                     /gene="ybbJ"
                     /locus_tag="ACHOTW_001698"
     CDS             complement(27919..28377)
                     /gene="ybbJ"
                     /locus_tag="ACHOTW_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9843826.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(28374..29291)
                     /gene="qmcA"
                     /locus_tag="ACHOTW_001699"
     CDS             complement(28374..29291)
                     /gene="qmcA"
                     /locus_tag="ACHOTW_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9843827.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            29437..30114
                     /gene="fetA"
                     /locus_tag="ACHOTW_001700"
     CDS             29437..30114
                     /gene="fetA"
                     /locus_tag="ACHOTW_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP9843828.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            30101..30880
                     /gene="fetB"
                     /locus_tag="ACHOTW_001701"
     CDS             30101..30880
                     /gene="fetB"
                     /locus_tag="ACHOTW_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP9843829.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(30943..31797)
                     /gene="cnoX"
                     /locus_tag="ACHOTW_001702"
     CDS             complement(30943..31797)
                     /gene="cnoX"
                     /locus_tag="ACHOTW_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP9843830.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(31858..32667)
                     /gene="ybbO"
                     /locus_tag="ACHOTW_001703"
     CDS             complement(31858..32667)
                     /gene="ybbO"
                     /locus_tag="ACHOTW_001703"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9843831.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(32657..33280)
                     /gene="tesA"
                     /locus_tag="ACHOTW_001704"
     CDS             complement(32657..33280)
                     /gene="tesA"
                     /locus_tag="ACHOTW_001704"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP9843832.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            33251..33937
                     /gene="ybbA"
                     /locus_tag="ACHOTW_001705"
     CDS             33251..33937
                     /gene="ybbA"
                     /locus_tag="ACHOTW_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP9843833.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            33934..36348
                     /gene="ybbP"
                     /locus_tag="ACHOTW_001706"
     CDS             33934..36348
                     /gene="ybbP"
                     /locus_tag="ACHOTW_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP9843834.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNYVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         8344 a        10266 c        10552 g         7282 t
ORIGIN      
        1 ctgcaattgc tgcaccgctg ccacaatggg cgaatgcccc agcggtaacg ccagttgccc
       61 aattatcttt acaggaaagt atattacgcg cctttgcgcg aaatcccggt gtcactcaac
      121 aggcggcgca gataggtatt ggcgaagcgc aaattgatga agccaaaagt gcctggtatc
      181 cgcacgttgg attaaccggc aacgcggggc cgtcccgaca aacggactcc agcggcaggc
      241 ttgataacaa cgtttcgtat ggcataaccc tgacacaact ggtgtatgac tttggtaaaa
      301 ctaacaacga tatcaatctg caaactgccg cccgtgacag ctaccgcttt aaattgatgg
      361 caaccttaac cgatgttgca gagaaaacgg cgactgccta catggaagtc agtcgttatc
      421 aggctttgtg cgatgcggct caacgcaata ttcactcgct ggaaaacgtc tacaacatgg
      481 cggcattgcg cgctaacgca ggcctgaact catcgtcgga tgaattacag gcccagacgc
      541 gtattgccgg aatgcgctca acgctggagc aatatcaggc gcagatggcg agcgccaaag
      601 cgcaactggc ggtgctcact ggcgtacagc cggaggcgat agccgcgcca cctgctgaat
      661 tagccgagca gccggtatcg ctgaagaata ttgattacca gtccatcccg ctggtgctgg
      721 cggcagaaaa cttacgccag tcagcacagt acggcgtgga aaaaacgaaa gcgcaatact
      781 ggccaacgct cagtattcag gggggtaaaa cgcgctacca gaccagcgac cgctcgtatt
      841 gggatgatca gctacaactg aacgttaacg cgccgctgta tcagggcggc gcggtttctg
      901 cccaggtgca acaggccgaa gggcaacaaa aaatctctgc ctcgcaggtc gaacaggcaa
      961 aactggatgt gctgcaacga gcgtctgtgg catatgcgaa ctggaccggc gcacggggtc
     1021 gtgaagaagc cggtttagcg caatccgaaa gtgcgcacaa aacgcgagat gtgtaccaaa
     1081 atgaatataa attgggtaaa cgcagtctga atgatctatt aaccgtcgaa caagatgtct
     1141 ttcaggcgca atcggctgaa ataaatgcca attatgatgg ctgggtcgcc gccgtaaatt
     1201 atgccgctgc ggtgaataac cttattccat tagcgggaat taaacagggc ttatacaacg
     1261 acttacccga cttgaaataa tcctgccatt attaatagtg ggccgttgtt aagtgattac
     1321 ctttgtctta tttagataaa tggactcgct aaggatgtgc gccatttatc aggaatgcca
     1381 ttatgagcct gattattgat gttatttcgc gtaaaacatc cgtcaaacaa acgctgatta
     1441 atcctggcga cgtcacggtt gttatttatg agccttccgt ggtgcaggtt catgctcagg
     1501 cctctgccgt tgcgcgttac gtccgtgaag gaaatgacct gctgatctat atgcaggacg
     1561 gcacggtgat ccgctgcaac ggttatttcc tgcaagcggc gaatacagct gaacaatcgg
     1621 aactggtgtt tgccgatggt caacagctaa cccatatcac ctttgccgat actgctgcgg
     1681 gtggattagc ccccgtagaa ctgactgccc agaccactgc gattgaaagc attgcgccat
     1741 ttcttgatac cgttgctcag accagcgcct tcccgtgggg ttggctggcg ggggcggcgg
     1801 taggtggtgg cgcgcttggt gcactgctgg caagcggtgg cgatggcgac tcgaaaacag
     1861 aagtgattaa taaccctacg ccacctgctg agcctggcaa cgccacacca tcatttttag
     1921 ttaccgataa tcagggcgat cagcgcggca ttctagccac caatgacatc accgatgaca
     1981 ccacgccaac ctttagcggc agcgggcagg cgggggcgac tattcagatt aaagacagta
     2041 acggcaatac tattgccagt actcaggtag acaacaacgg tcactggagt gtctcgttac
     2101 ccacgcaaag tgcaggtgaa catacctggt cagtggtgca aattgtcggc agtaccatca
     2161 ctgacgccgg ttcgataacg ttaaccatcg acaatagtca ggccagcgtg caggttgcca
     2221 ccaccgcagg cgataacatt attaacgcca gcgaacaggc cgccgggttt acgctttctg
     2281 gcaccagtag ccatctggcg cagggaacag aactcaccgt tacgctaaac ggcaaaacct
     2341 acacgaccag cgtaggcgct aacggtgcct ggagcgtgca ggtgccgacc gccgatgcac
     2401 aggcgttagg cgaagggaat caggcggtgc tggtcagtgg gaaagacgcc acaggcaata
     2461 cggtcaccgg cgcgcagcta ctaacggtcg atacccaacc gccaacgctt gccatcaaca
     2521 ccatcgctca ggacaacatt atcagtgctg cggaacataa cgtcgcgctg gtactgagcg
     2581 gcacgtcgaa tgcagaagcg gggcaaaccg taacactgac cgtcaacggg aaaagccata
     2641 cagcaaccgt cggtagcgac ggaacctggc aagtgacgct gcctgccacg gaagtccagg
     2701 cactggcgga gggtaattac gctgtcaatg ccagtgtcag cgatcgggca gggaacacca
     2761 ccagccacag cgcgaatttc acggtagaca cctcagcacc cgtggtcagt gttaataccg
     2821 tggcgggcga cgatattctt aataatgccg agcaggccgt cgcgcagatc atctccggac
     2881 aagtcagcgg tgcttctcca ggcgatacgg taacggtgaa attgggcact catgtcctga
     2941 cgggcatcgt gctggcagat ggcagctgga atgtggcgct ggacccagcg gtaacccgca
     3001 cgctggatcg cggagccaat acgattttcg tcaccgtgac agatgctgca ggaaatactg
     3061 gcgcggcgtc tcgagcaatc acgctggtcg gtgtttctcc gttgatcacc attaacaccg
     3121 tctccggcga tgacattatc agtggcgcag aaaaaggtgc gccactgacc cttaccggta
     3181 gcactcaaca ggctgagaca ggacaaaccg tcacagtaac cctggctgga cagagtttta
     3241 ccactaccgt gcaggccgat ggctcctgga gtctgacggt acctgccgcc gcgatgggaa
     3301 atctgcctga cggcgcggtg gcgattaccg cttctgtgac ggatctcagc ggcaataccg
     3361 gcaacacttc ccgcaccatt accgtcgata gccaggcccc ggccttaagc attgatccac
     3421 tgaccgctga taacatcatt aacgccgccg aaagcgggca ggatctgccc atcaccggca
     3481 ccaccgacgc tcagccgggg cagacggtga ccgttacgtt aaatgggcag acgtatcagg
     3541 gcgtcgtgca gccagacggc acctggagcg tgactgtgcc cgccgccaac gtgggcgcac
     3601 tggctgacgg caacgctacg gtcaccgcca gcgtgaacga tgtcgccggt aatccgagca
     3661 gcgtttcacg cgtggcgctg gtggatgcca cgccgccggt ggtaaccatt aatccggtgg
     3721 cgaccgataa cgtcatcaac acgccggaac atgctcaggc gcaaatcatc agcggcacgg
     3781 ttactggcgc tcaggcgggc gatatcgtca ccgtgacgct gaataatgtg gattacacca
     3841 cggtggtgga tggttccggc aactggagtc tgggcgttcc ggcctcggtg gtcagtgggc
     3901 tggcggacgg cagttatcct gtcagcgtct cggtaaccga caaagccgga aacacgggca
     3961 gccagtcatt gaccgtcacg gtcaataccg ccgcgcccct tatcggcatt aacagcattg
     4021 cgggcgatga tgtgattaac gccagcgaaa aaggggccga tctccagatt accggcacca
     4081 gcgatcagcc tgttaacacc gccatcaccg tgacgctgaa cgggcaaaat tacaccacca
     4141 cgaccgacgc ctccggcaac tggagcgtca ccgttccggc atcggcggtt acagcattag
     4201 gccaggccaa ctatacggta acggcggcgg tgaccagcga tatcggcaac agcgccactg
     4261 ccagccataa cgtgctggtc gacagcgcgc tgcccggtgt gaccattaat ccggtggcaa
     4321 ccgacgatat tattaacgcc gccgaagcgg gcgtggcgca aaccatcagc gggcaggtga
     4381 ctggcgcgga agatggcgac acggtaacta ttacgttggg tggtaatact tatacggcga
     4441 cggtgggcag caatctcacc tggagcgtgg acgttccagc ggcagatatt caggcgctgg
     4501 gaaatggcga tttaacggtt aatgcctcag tcaccaatca aaacggcaac accggcagcg
     4561 gcacgcggga tatcaccatc gacgccaatc tgcccggcct gcgggtcgat acggtggcgg
     4621 gcgatgatgt ggtcaatatc atcgagcacg ggcaggcgct ggtggtcacc ggcagcagct
     4681 cggggctggc tgaaagcacg ccgcttaccg ttacgattaa taatgtggaa tacaccactg
     4741 cggtgcaggc cgatggtagc tggagcgtgg gcgtcacggc ggcgcaggtt agcgcctggc
     4801 ctgcggggac ggttaatatt gccgtttcag gggaaagtag cgccggaaac tcggtgagca
     4861 ttacgcatcc ggtgacggtg gatctcactc cggcagcgat caccatcaac accatcgcca
     4921 cggacgatgt gattaacgcc gcagaaaaag gcgctgattt aaccctttcc ggcaccacca
     4981 ctaacgtaga acccggtcaa accgtcaccg tcacctttgg cgggaaaaat tacactgcca
     5041 gcgtagcgag cgatggtagc tggactgcca ccgtacccgc cgccgatctg gcgtcattac
     5101 ccgagggcag cgcctccgca ctggccagcg tcagcaatat caacggcaat agcgcctcgg
     5161 cggtgcacaa ctacagcgtc gacagcagcg cgccaaccat cattatcaat accgtcgcca
     5221 gcgacaatat cgtcaacgcc agcgaagccg atgcgggcgt gacggtgagc ggcagtacca
     5281 ccgccgaagc ggggcagatt gttacgataa cgcttaacag cccgaccgtg cagacctatc
     5341 aggcaacggt gcaggcggac ggcagctgga gcatcaatat tccggcggca gatcttgagg
     5401 cattgaccga tggcagccac accctgaccg ccacggtcaa tgacaaagcg ggcaatccgg
     5461 cgagcaccac gcataatctg gcggtggatc tcaccgttcc ggtgctgacc atcaacacca
     5521 ttgcgggcga tgacattatt aacgccaccg aacacgggca ggcgctggtg atttccggtt
     5581 ccagcaccgg cggagaagcg ggggatgtcg tcaccgtcac gctaaacagt aaaacctaca
     5641 ccaccaccct ggacgcctcc ggcaactgga gcgtcggcgt tccggcggcg gatgtcacgg
     5701 cgcttggcag cggcccgcaa actgtcaccg ccacggttac cgatgcggca ggcaacagcg
     5761 acaattagac acacacggtc accgttaacc tcaccgcacc aaccattgac atcaacacca
     5821 tcgccaccga tgacataatt aacgccagcg aaaaaggcgc ggatctgcaa atttccggca
     5881 ccagtaatca gccagcaggc accacgatta cgctggcgct taacgggcaa aattacaccg
     5941 cgacgacgga cgctgcaggc aactggagca ctacggtgcc agcttccgca gtaggtgcgc
     6001 tgggcgaagc cagctacacg gtgacggcga acgtcaccga cagcgcaggc aacagcaatt
     6061 ccgccagcca taacgtgcag gtcaataccg cgctgcctgg cgtcaccatt aacccagttg
     6121 cgaccgacga tattattaac gccgccgaat cgggcaatgc gcaaaccatc agcggccagg
     6181 tgacgggggc ggcggcgggc gatacggtta ccgtaacgct tggcgggaaa acttacaccg
     6241 ctaccgtgca ggggaattta agctggagcg tggacgttcc ggcggcggat attcaggcca
     6301 tcggcaatgg caatctgacg gttaacgctt cggtgaccaa cggcgttggc aatactggca
     6361 gcggttcgcg agatattact atcgacgcca acctgccagg tctgcgcgtt gacaccgtgg
     6421 cgggcgatga tgtggtcaat agcatcgagc acgctcaggc gctggtgata actggtagca
     6481 gcagcgggct ggcggcgggc gcggcgctga cggtggtgat taacacggtc acttacgctg
     6541 caacagtatt agccgatggc acatggagcg ttggtgttcc ggcggcagac gtgagtaact
     6601 ggcctgcggg tacggtgaat atcacggtaa gtggcactaa cacagccgga acaacatcca
     6661 ccatcaccca tccggtcacc gtcgatctgg cggcggtggc gatttccatt aacaccgttt
     6721 ccggcgacga tgtgattaac gccgccgaaa aaggggcaga tttaaccctt tccggcagca
     6781 cctccggcgt ggaagtgggg caaacggtca ccgttacctt tggcgggaaa acctacaccg
     6841 ctaccgtagc gggcgatggt agctggacaa ccaccgtacc cgccgccgat ctcagcgtgt
     6901 tacgcgacgg cgacgccacc gtgcaggcca gcgtcagcac tattaacggc aacacggctt
     6961 cggcaaccca cgcctacagc gtcgatgcca cggccccgac gcttgccatt aacaccatcg
     7021 ccaccgacga tattctgaac gctgccgagg cgggcaatcc gttaaccatc agcggtagca
     7081 gcaccgccga agcggggcag acggtaaccg tcacgcttaa tggtgtgact tacagcggct
     7141 ccgtccaggc ggacggcagc tggagcgtca gcttaccgac ggcggatctc agcaatctga
     7201 ccgccagcca gtacaccgtt agtgcctcgg taagcgataa agcgggtaac ccggcgtccg
     7261 ctaaccacgg gctggcggtg gatctcaccg tgccggtgct gaccatcaac accgtctccg
     7321 gcgatgacat tattaacgcc gccgaacacg gacaggcgct ggtgatctcc ggctccagca
     7381 ctggcggcga agcgggtgat gtcatcaccg tcacactaaa cagtaaaacc tacaccacca
     7441 tgctggacgc ttccggcaac tggagcgtcg gcgttccggc ggctgacgtc actgcgcttg
     7501 gcagcggccc gcaaactatc actgcggcaa ttaccgacgc ggcaggcaac agcgatgacg
     7561 ccagccgcac ggtcaccgtg aatctcgccg cgccaaccat tggtatcaac accatcgcca
     7621 ccgatgacgt gattaaagcc acggaaaaag gcgcggacct gcaaatcacc ggcaccagta
     7681 atcagcctgc gggcaccacc attacggtga cgctgaacgg gcaaaattac accgctacta
     7741 ccgatagcaa cggcaactgg agcgccacgg tgccagcgtc agcggttagc gcattgggtg
     7801 aagccaacta cacggtaacg gcaaacgtca ctgatacggc aggcaacagt aattccgcca
     7861 gtcataatgt gctggtcaac agcgccttgc ctgccgttac cattaacgcg gtggcgactg
     7921 acgatattat taacgctgcc gaatcgggaa atgcgcaaac catcagcggg caggtgacgg
     7981 gcgcagcgca gggggatacg gttaccgtta cgctgggcgg caacacctac accgccacgg
     8041 tgcagtctaa tttaagctgg agtgtggacg ttccggcggc ggatattcag gcgctgggca
     8101 acggcgacct gacggttaat gcctcggtca ccaatggcgt cggcaacacc ggcagcggct
     8161 cgcgcgatat cacgatcgac gccaatctgc ctggcctgcg ggtcgatacc gtggcgggcg
     8221 atgatgttat taacagcatt gagcacaatc aggcgctggt gatcaccggc agcagcagcg
     8281 gattaacggc gggaacggcg ttaacggtcg agattaacaa cgttacttat ggcgcgacgg
     8341 tattagccga cggcacgtgg agcctcggtg ttccggcggt agacgtcagc aactggccag
     8401 cgggtacggt gaatatcacg gtaagcggca ccaacagtgc cggaacaacc tccaccatta
     8461 cccatccggt taccgtcgat ctggctgggg tcgccatcac cattaacact ctctctggtg
     8521 atgatgtgat caacgccgtc gaaaaaggcg aaacgctggt cgtaagcggc agcaccagcg
     8581 gtgtcgaagc cgggcagacg gtgaccgtca cctttggcgg caaaaattac accaccacag
     8641 tggaagctaa cggtagctgg acggtgaatg tgccgcctgc cgatctcgct gcgctaccgg
     8701 acggcgcggg caacgtgcag gcgagtgtca gtaatattaa cggcaacagc gcccaggccg
     8761 atcgcgcgta tagcgttgat gccaccgcgc cgcttgtgac catcaacacc atcgccagcg
     8821 acgatatcct taacgtgagc gaagctggcg cggggatcac catcagcggc actaccacgg
     8881 cgcaggccgg gcagacgctc accgtcacgc tcaataacaa cacgtaccag accaccgttc
     8941 tggcggatgg cacctggagc gtgaatgttc cggcagcgga tttaagcgga ttaaccgcca
     9001 gcagttacac cgtgaccgcc acggtgagcg acaaagcggg taacccggca agcgccgacc
     9061 acgcgctggt ggtagatatc actgcgccgg atctcaccat taacaccgtc gcgggcgatg
     9121 acattatcaa cgccatcgaa catggtcagg cgttggtggt cagcggcacc agtacgggcg
     9181 cggcggcggg tgatgtggta accgtcacgc tgaacggtaa aaactacacc accacgctgg
     9241 atgcctccgg taactggagc gtgggcattc cggcggcgga tgtcacggcg ctggcgaccg
     9301 gtagccagac catcaccgcc agcctgagcg atcgcgcggg caacagcgac agcacgactc
     9361 acgatgtgac cgttgatctt agcggcccga cgctgaccat taacaccgtc tccggcgatg
     9421 acattatcaa cgccgctgaa atcgttgtgg cgcagaccat cagcggtcag gtcacgggaa
     9481 cggcggttgc cgggaatacg gtgattgtca ccattggcgg caatcaatat aacgccaccg
     9541 tgcagtcaga tttaagctgg agcgtcagcg taccggcgaa cgttttgcag gcgctgggta
     9601 acggtgaact gaccatcagc gcctcgttga ccaattccgc aaataatacc ggcaccgcga
     9661 cgcacgatat cgtgatagat gccaacctgc caggtctgcg cgtcgatacc gtggcaggcg
     9721 atgatgtgat taacagcatc gagcacactc aggcgttggt gatcaccggt agcagtagcg
     9781 gactggcggc gggcgcggcg ttgacggtgg ttatcaatag cgtcacctac ggcgcaacgg
     9841 ttttagcgga tggtagctgg agcgttggtg ttccggtggc agatgtcaca aactggcctg
     9901 cggggacggt caatattgcc gtctctggca ccaacaccgc cggaaccaca accagcatta
     9961 gccatccggt cacggtcgat ctcgctgccg tggcgatcac catcaacacc ctttccactg
    10021 acgatgtgat taacgccgcc gagaaaggct ctgatttgca gctctccggc accacctccg
    10081 gcgtggaagc ggggcaaacc atcaccgtta tcttcggcgg caaaagctac accaccacgg
    10141 ttgccgcgga taatacctgg gggctgacga tccctgccgt cgatgttgca accttgccag
    10201 acggcgcagc aaacgtccag gccagcgtca gcaatgtcgc agggaacagc acccaggcaa
    10261 cgcatgctta cagcgttgat gccactgcgc cctccgttac catcaacacc atcgccacgg
    10321 acgatattct taacgccgcc gaagcgggat cggcgctgac catcagcggc accagcacgg
    10381 cggaagccgg gcagacggtg accgtaacgc taaacggcgt taattacagc ggcaatgtgc
    10441 aggcagacgg gagctggagc gtcagcgtac caaccggcga tttagctagc cttaccgcca
    10501 gctcgtatac cgttaacgcc tcggtcagcg acaaagccag aaattcggct tcggcaacgc
    10561 ataatctgac ggtggacctt gccgctccgg tcgtcaccat caacacggtg gcgggcgatg
    10621 acatcattaa cgccacggaa cacggacagg cgcagatcat cagcggctcg gcaacgggcg
    10681 cgactaccgg taatacggtt tccgtgacga ttggcacgac cacctatacc accgtgctgg
    10741 acgccaacgg caactggagc atcggcgtgc ctgccagcgt gatttccgcg ctggcgcagg
    10801 gcgatgtgac cattaccgct acggtcaccg actccgcagg caacagcggc acggcctcgc
    10861 acactgtcac cgtggcgctc ggcgctccgg tgctcgccat taacaccatt gccgtcgatg
    10921 acatcatcaa cgccgcggag aaaggcgcgg atctggcgat taccggcacc agcaaccagc
    10981 ctgcgggcac gcagattacc gttacgctca acgggcaaaa ttacaccacc actgccgatg
    11041 cttccggtaa ctggagcgtg accgttccgg cgtcacgggt gagcgccctc ggtgaagcca
    11101 cctacacggt gaccgcagcc gccactgacg ccgatggcaa cagcggttcc gccagccata
    11161 acgtacaggt taataccgcg ctgccgggcg tcaccattaa cgtggtggca acggacgata
    11221 ttattaacgc cgccgaagcg ggcgtggaac agaccatcag cgggcaggtg acgggtgcgg
    11281 cggcaggcga cacggtgacc gtcacgctcg gcggggcgac ttacactgcc acggtgcagg
    11341 cgaatttaag ctggagcgtc gatgttccgg cctccgcgct acaggagttg ggcaacggcg
    11401 aactgaccat ttcggcttcg gtgacgaaca gcgtaggcaa tactggtaac ggcacgcgcg
    11461 aaatcaccat agacgcgaat ctccccggtc tgcgggtcga caccgtggcg ggcgatgatg
    11521 tggttaatat tatcgagcac gggcaggcgc tggtgattac cggcagcagc tccggtctgg
    11581 cggcgggcag caacgtcacg ctgaccatta acgggcaaac ctatgttgcg gcggtgctgg
    11641 cggatggcac ctggagcgtc ggcgttccgg cggtggatgt cagcgcctgg cctgcgggat
    11701 cggtgacgat tgcggcgagc ggtagcacct ctgccggaaa tccggtaagc gttacgcatc
    11761 cggtgacggt cgatctctcg gcggtggcgg tgagcatcaa cgccattacc gccgatgatg
    11821 tgatcaacgc tgccgaaaaa ggcgcggcgt taacgctctc cggcagcacc tctggcgttg
    11881 aagccggaca aacggttacc gtcacctttg gcggcaaaac ttacagcgcc acggtggctg
    11941 cgaatggttc ctggagcacc tcggttccgg cggcagatat ggcggctctg cgtgatggcg
    12001 atgccagcgc acaggccagc gtcagcaatg ttaacggcaa cagcgccacc acgacccacg
    12061 cttacagcgt tgatgccagc gcgccaacgg tgaccattaa taccattgcg ggcgatgata
    12121 ttcttaacgc cgccgaagcc ggagcggctc tgaccatcac cggcagcagc acggcggaag
    12181 cggggcagac ggtgaccgtc acgctcaatg gcacaaacta caccggcacc gtacagacgg
    12241 acggcagctg gagcgtcagc gtaccgtcag ccgacttaag caccctgacc gccagcaact
    12301 acaccgtgaa cgcggcggtg agcgacaaag ccggaaaccc ggcctcggtt aatcacaacc
    12361 tgacggtgga tacgtccgtt ccggtcgtca ccatcaacac ggtggcaggc gatgatgtga
    12421 tcaacgcgac ggaacacgcc caggcgcaga tcatcagcgg ctccgccact ggagcggcaa
    12481 ccggtagcac cgtgacggtg actatcggca caaatacctt taccacggtg ctggatgcca
    12541 gcggcaactg gagcgtcggc gttccggcaa gcgtcgtctc ggcactggcg aatggcacgg
    12601 tgaccatcaa tgccagcgtc accgatgccg gaggaaacag cggcagcgct acccatcagg
    12661 tgacggtcaa taccgggctg ccgaccatta cctttaacgc catcagcggc gataacatcc
    12721 tgaacgccga tgaaaaaggc cagccgttga ccatcagcgg cggcagtacg gggctggcga
    12781 cgggcgcgca ggtcaccgtc acgctcaacg gtcacaacta cagcgccacc accgacgcat
    12841 cgggcaactg gaccttaacc gtgccggtga gcgatctggc ggcattaggt caggccaact
    12901 atacggtcag cgccagcgcc accagtgcag caggcaacac cgccagcagc caggcgaatt
    12961 tactggtcga cagcggcctg ccggacgtca ccatcaacac cgtggcaggc gacgatatta
    13021 tcaacgccgc cgaagcgggg gccgatcaaa ccatcagcgg ggtggtgact cgcgccgccg
    13081 ctggcgatac ggtcaccgtg acgctgggcg ggaacactta caccgctacg gtacagagca
    13141 acttaagctg gagcgtcagc gttccgacag ccgatctcca ggcgttgggc aatggtgatt
    13201 tgaccattac cgcctcggtc accaacgcta atggcaacac cgggagcggc acgcgggata
    13261 tcaccattga tgccaacctg ccggggctgc gcgtagatac cgtggcgggc gatgatatcg
    13321 tcaacagcat cgagcacggg caggcgctgg tgatcaccgg cggcagtagc ggcctgaatg
    13381 caggtgctgt gctgacggtt accatcaaca gtgtggcgta ttccgccacg gtgcaggcgg
    13441 acggaagctg gagcgttggc attccggcgg caaacgtcag cgcctggcct gcggggccgt
    13501 taaccgtgga ggtagacggg caaagcagcg ccaataaccc agtcagcgtc agccatccgt
    13561 tcaccgtcga tttaacggcg gtggcaatca gcatcaacac cgttgccagc gacgacgtga
    13621 ttaacgccgc agaaaaaggc accaatctga ctctttccgg cagtaccagc gggattgaga
    13681 gcgggcaaac cgtcaccgtc acttttggcg gtaaaaccta cactgcaagc ctcgccgcca
    13741 acgggagctg gagtgtaaac gttccggcgg cagatctggc aactctgcca gagggcgcgg
    13801 cgaatgtgca ggccagcgtt agcagcgcga gcggtaacag tgcctcggcg acccatgcgt
    13861 atagcgttga cgccagcgcg ccgacgctca ccattaacac catcgccagc gacgatatcc
    13921 ttaacgccgc agaagccgga agcccgctca ccatcagcgg caccagcacc gccgaaaccg
    13981 ggcagacggt gaccgtcacc cttaacggcg caacctacac cggcactgtg caggcggacg
    14041 gtagctggag cgtcagcgtt cccacttcag ccctgggcgc gctcaacgca agcaattaca
    14101 ccgtcagcgc cacggtcaat gacaaagcgg gcaaccccgg cagcgccagc cataatctgg
    14161 cggtagacac caccgcgccg gttctcacca ttaacaccgt ggcgggcgat gacatcatca
    14221 acgatgccga acatgcgcag gcgctggtga tctccggcac cagtagcggc ggggaagcgg
    14281 gcgatgtggt gagcgttgtg ctcaacggca aaacctacac caccaccctg gatgcctccg
    14341 gcaactggag cgttggcgtt ccggcggcag atgttacggc gctgggtagc ggtgcgcaga
    14401 ccatcaccgc cagcgtcagc gatcgggcag gcaacagcga cgacgccagc cgcaccgtga
    14461 ccgtcagcct cagcgcgcca gtgattagca tcaacaccat cgcgggcgat gatgtgatca
    14521 acgcgacgga aaaaggatct gatctggcgc tttctggcac cagcgatcag cctgcgggta
    14581 cggcgatcac cgtcaccctg aacggacaaa actacagcgc caccacggat gcctccggca
    14641 actggagtgt taccgtgcct gcctcggcgg tcagtgcgct gggcgaagcg acctacagcg
    14701 tgacggcgag cgtcaccaat gctcagggta acagcagcac cgccagccat aacgtgcagg
    14761 ttaataccgc gctgccgggc atcaccatta atccggtggc gacggacgat attatcaacg
    14821 cttcagaggc aggcagcgcg caaactatca gcggccaggt aaccggtgcg gcggcgggca
    14881 gcaccgtcac cgttgaactg ggcggtaaaa cttacaccgc caccgtccag gctgatttaa
    14941 gctggaatgt gagcgtacct gccgccgact ggcaggctct gggtaacggt gagttaacgg
    15001 ttaatgcctc ggtgaccaac gccgttggca acaccggtag cggcacgcgc gatatcacca
    15061 tcgatgccag tctgcctggc ctgcgggtgg ataccgtcgc gggtgatgat gtggtcaata
    15121 tcatcgaaca cgctcaggcg caggtgatca ccggcagcag ctccggcttt gccgcaggca
    15181 cggcgttgac ggtggtgatt aacaaccaaa cttacgccgc cacggtgttg gcgaacggta
    15241 gctggagcgt cggcgttcct gcgacggatg tcagtaactg gcctgcggga acgctgaata
    15301 ttaccgttag cggggcgaac agtgccggaa cgcaaaccag cattacccat ccgctgactg
    15361 ttgatctcac cgccgtcgcc atcagcatga acagcatcac cagcgatgat gcgattaacg
    15421 ccgccgaaaa aggcgcggcg ttaacgctct ccggcagcac gtccggcgtc gaagcggggc
    15481 aaaccgtcac cgtcaccttt ggcggcaaaa cctacaccac cacggtggcg gcaaacggta
    15541 gctggagcac caccgttccg gcggcggatt tggcggccct gcgtgatggc gatgccagcg
    15601 cccaggtgcg ggtgactaac gtcaacggca acagcgccac agcaacgcac gaatacagcg
    15661 tcgatagcgc cgcgccaacg gtgaccatca acaccattgc cagcgataac atcatcaacg
    15721 ccagcgaagc ggcggcgggc gtaacggtgt ccggcaccag caccgcgcaa accgggcaga
    15781 cgctcaccgt cacgcttaac ggcactaact accagaccac ggtgcagaca gacggcagct
    15841 ggagtttaac gctgcccgcc agcgacttaa ccgcacttgc taataacggc tacaccctga
    15901 ccgccacggt cagcgatctg gcgggtaacc ttggcagcgc cagcaaaggc gtgaccgtcg
    15961 ataccactgc gccggtgatc agttttaaca ccgtggcggg cgatgatgtg attaacaacg
    16021 tcgaacacat tcaggcgcag attatcagcg gcaccgccac gggcgcggtg gcgggcgacc
    16081 gcctggtggt gaccatcgcc gggcagcagt atgtcaccag caccgatgcc agcggcaact
    16141 ggagcgtcgg cgtgcctgcc agcgtgattt ccggccttgc ggatggcacg gtgaccatca
    16201 gcgccaccat taccgatagc gcaggcaaca gcagcacgca gacgcacaac gtacaggtga
    16261 acacggcggc agtgtcgctc tcggtcagca ctatcagcgg cgataacctt attaacgccg
    16321 ccgaagcggg cagtgcgctg accctgagcg gcactggcac taatttcgcg acaggtacag
    16381 tggtgaccgt gttgcttaac ggcaaaggct acagcgccac cattcagagc aacgggagct
    16441 ggagcgtgaa cgtgcctgcg gcggatgttg cggcactcag tgatggcacc agctacacgg
    16501 ttagcgcctc cgctcaggac agtgccggaa acagcgccac ggcctcgcgc agcgtggcgg
    16561 tggatctcac cgcgccggtt atcagcatta acaccgtttc gacggacgac cgcctcaacg
    16621 ccgccgaaca gcagcagccg ttaaccctta acggctcgac cagcgcggaa gtggggcaga
    16681 cggtcaccgt cacctttggc ggcaaaacct ataccgccac ggttgccgcc aatggtacct
    16741 gggcgctgaa cgtgcctgcg gtggatctgg ccgcgctcgg gcagggcgcg cagaccatta
    16801 ccgccagcgt gaacgatcgc gccggtaacc ccggacaggc cacgcacgcc ctgaccgtcg
    16861 ataccgttgc gccaacggtc accatcgcca cggtggcggg tgacgatatt atcaacaacg
    16921 ccgagcagct tgccgggcag accattagcg gcaccaccac cgccgaagtg ggccagacgg
    16981 tgaccgttac ctttaacggg caaacctgga gcgcaacggt tggcagcggc ggaagctggt
    17041 cggtgtttat tccggcgcag cagtttgccg gattaagcga cggcagctac accattagcg
    17101 cgacggttag cgatcaggcc ggaaaccccg gcagcgccag ccgtggcgtg acgctgaacg
    17161 gcgatgtacc tactgtcacc atcaacacct ttgcgggcga cgatgtggtg aatgcagcgg
    17221 aacacggctc atcgctggtg atcagcggca ccaccaccgc gcccgtcggg cagacgctga
    17281 ccctgacctt aaacggcaaa acctacacca ccacggtgca gacgggcggt agctggagct
    17341 acaccctcgg cagcgccgat gtcaccgcgc tggcggacgg caacgcctac gtgattaacg
    17401 cctcggtgag caatgccatt ggcaacaccg gtagcagtaa tcacaccatt accgtcgatc
    17461 tcagcgctcc ggcgatgggc attaatatcg attccctgca agccgacact ggccttagcg
    17521 ccagcgactt tatcaccagc gtcagtccgg tagtggtcaa cggctcgctc accgccgcgc
    17581 ttgccagtaa cgagacggcg caaataagta tcgacggcgg cacaacctgg accacgctta
    17641 ccgttaccgg cacgacctgg cgctataacg acagccgcac gctgaccgat ggcaactatc
    17701 tctatcaggt gcgggtgatt gacgcagcgg gcaacgttgg cgcgaccgac agccagaatg
    17761 tggtgatcga cactaccgcg ccagatcccg cggtgaaaac catcgccatc agcgcgatca
    17821 ccaccgatat gggcttgatc actaacgatt ttgtcaccag cgacacgacg cttgcggtga
    17881 gcggcacgct gggggcgacg ctttctgccg gtgagttcgc gcaaatcagc ctcgacggcg
    17941 gcgtcacctg gactacgtta accgtcgttg gcaccagctg gagctatgcc gatggtcaca
    18001 cgcttactga cggcacctgg aactacacgg tgcgggtggt ggatctggcg gggaacgttg
    18061 ggcagaccgc aacgcaaaac gtggtggtcg acaccaccag cccggaagcg gcgaaaagta
    18121 tcaccattac cggtatcagc gatgacaccg gaaccagcag cagcgatttt attaccagcg
    18181 acaccacgct taccgtgcgc ggcgtattgg gcgcggcgct cggcgctaat gagttcgcgc
    18241 aaatcagtac cgacaacggc gcaacctggg tgaacgtgac cgtcgccgca gacagcctga
    18301 actggagtta cgttgacgga cgaaccctca ccaacggcac caccacctgg caggtgcggg
    18361 tggtcgatct ggcgggcaac gttggcgcaa cgagcagcca gtcggcgctg atcgataccg
    18421 ttaacccggc gcaggtgctc accatcgcca gcatcagcac cgacacgggg agttcggcaa
    18481 ctgactttat caccagcgac accatgctca cgctgaccgg ttcgctgggg gcggggcttg
    18541 ccagcggcga agtggcgcag attagccttg atagcggcgc gacctggaca acgctcacca
    18601 ccaacggtac acagtggact tacaccgaca gccgcacgct gaccgacggc agctacgttt
    18661 atcaggtgcg ggtgctggat ctggcgggga acaccggccc ggtggtgtcg aaaacggtgg
    18721 tggtcgatac gattaacccc accgccacac caacgattgt gtcgtatacc gatgatgtcg
    18781 ggcagcggca ggggacatta agcagttcgc aggccaccga cgacactacg ccgctgctga
    18841 acggtgtact ttccgcgccg cttgccagcg gtgaagtggt ttacctctac cgtaacgggc
    18901 tgctgttagg ggcggtgacg atggtcggcg ctctgaactg gacctacagc gacagcgggc
    18961 tggtgagcgg tgcctatacc tacagcgcgc gagtggtgga tttggcgggg aatatcacct
    19021 cctccagtga ttttgtcctg acggtcgata cctctattcc gaccacgctg gcgcaaatca
    19081 ccagccagac cacgcgcgat actacgccga ttattagcgg ggtgatcacc gccgcgcttg
    19141 ccagtgggca gtatgttgaa gtggtgatca acggcaaaac ctacacctct gaaccgggcg
    19201 gcgcggtagt ggtcgatccg gcgcacaaca cctggtatgt acagttgccg gataccgatg
    19261 cgctgacagt ttccgcgacc gcctataccg ttactgcgca ggtaaaaagt tcggcgggta
    19321 acggcaataa cgccaatatt agcaacggca cggtgacggt taacgcggcg attgattaca
    19381 caccgacctg gactaccgcc agcaaaacca ccgcctgggg gctgacctac ggcctcgact
    19441 cgcacgggat gtggacggtg ctggcaaacc agcaggtaat gcaatcgact gacccactca
    19501 cctggtcgaa gaccgcgctg acgctgtatc agagcggcaa caactacgcc accagctcca
    19561 ttgccgatta cgaccgtaac ggcacgggcg atctgtttat cacccgtgat gactacggta
    19621 cgggctatat taacggcttt accaataacg gcgatggcac cttttccagc gctattcagg
    19681 tcaccgtcgg caccctgacg tggtacggct cgattgtggc atttgataaa gagggcgacg
    19741 gctatctcga cttctggatt ggtgacgctg gcgggccgga ctccaacacc ttcctgtgga
    19801 acaacgcagg cacgctggta ggcaactcca ccacgtcgaa cagcggcggt agcgccacgg
    19861 tgggcggggc ggtgacgggg tatctttcgc tcaacgaagg ttctggcgtc gatctgaaca
    19921 atgacggcag gatcgacctg gttcagcaca cctataacct gaacaactat tacacgctgt
    19981 cttcgctcat caaccagggg aatgggacgt ttgtctgggg gcagaacacc accaatacct
    20041 tcctgagcgg ggcgggcagt ggcgctatga gcagcagcgt ttccatgacc tgggccgatt
    20101 tcgatggtga cggcgatatg gatctcttcc tgcccgccag ccagggaaga gctaactacg
    20161 gctcgctgtt attcaacacc aacggcgtac tgggttgccc ggtggcggtg ggcgcaacgg
    20221 caaccaccta cgccagccag tttagcctgg cggtggactg gaaccacgac ggcctgatgg
    20281 atatcgcccg tatcgcccag accgggcagt cgtatcttta tactaacgtc agcaacgcca
    20341 gcaactggac gcaatcggcc ctcggcggca gccagagcgg taccaccagc ggcgtggcgg
    20401 caatggacta cgactgggac ggcgcggtgg atgtgctggt gtccaaacag tcgggcagcg
    20461 tgttcctgag ccgcaacacc aacacggtga gctacggcac ttcgctacac ctgcgcatca
    20521 ccgatcccaa cggcattaac gtctattacg gcaataccgt gaagctgtac aactcggcgg
    20581 gagtgctggt cgccacgcaa atcatcaacc cgcagtcggg tatgggggtt aacgacacct
    20641 cggcgctggt caacttctac gggctgaatg ccggagaaac ctacaacgcg gtgctgatca
    20701 aatccaccgg caccaccgcc agcaatatcg accagacggt caacaccagc tggggcggtt
    20761 tacaggccac cgatgccact cacgcttacg atctcagcgc tgaagcgggt accgcgagca
    20821 acaacggcaa gttcgtcggc accggctata acgacacctt cttcgccacc gcaggcaccg
    20881 acacttacga cggttccggc ggctgggtgt acagctccgg caccggaacg tggctggcga
    20941 acggcgggat ggacgtggtt gatttccggc tttcgacggt gggcgtgacg gctaacttaa
    21001 gcagcactgc cgcgcaggcc accggtttta acacctcgac atttaccaat atcgaaggca
    21061 tttccggctc gaattttaac gacattctga ccggcagcag cggcgataac caactggaag
    21121 ggcgcggcgg caacgacacg ctcaacatcg gcaacggcgg ccacgacacc ttgctctata
    21181 aactgctcaa cgccagcgac gccactggcg gcaacggctc agacgtggtg aatggcttta
    21241 cggtagggac atgggaaggc accgccgaca ccgatcgcat tgatattcgt gaactgttac
    21301 agggcagcgg ctacaccggc aacggcaaag ccagctacgt caacggcgtg gcaacgctgg
    21361 atgcgcaggc cggaaacatc ggtgactttg tcaaagtcac ccagagcggc agcgacacca
    21421 tcgtgcagat cgaccgcgac ggcacgggcg gcacttttgc gacaactaac gtggtcacgc
    21481 tgacgggcgt gcacaccgac ctcgccacct tgctggcgaa tcatcagttg atggtggtgt
    21541 agataatgaa aaaaaatgcc cagtccgtgg aagcctggct ggaggcgatg atcgccgtgg
    21601 cgcgctatta tcggctcgat ttttctcagg agaatgtgcg cgcgaccgtc aactgggagc
    21661 gcgacagtaa gcgggaggag ttactcaccg acatggcgcg gcagttaggg atggggttgc
    21721 ggttggtgga gttttccgcc gattcgctta acccctggcg tctgccgctg atcgcggtgt
    21781 ttgataatca gcagattggc gtgatcaccc gtcgcgacaa tcatgacaat atcagcgttc
    21841 agttcagcgg tgacgaaggg ctggaaacta cacttaacgt cgcggatatt gaagataaaa
    21901 tcgtcgaact ggcgctgctg cgccctctca gcgccatccc tgacgcgcgg gtggatgact
    21961 atattcgccc gtatcaggcg aactggttct ggagtctttc gctaaaagac tggcggcgct
    22021 acggcgatat tatgctggcg tcactggtgg cgaacgtgct ggcgctggcg gcgatgattt
    22081 tctcgatgca ggtttacgat cgggtggttc cggcgcaatc gtacccgacg ctatgggtgc
    22141 ttttcgccgg ggtgatgatg gcgatcctgt ttgagttctg tatgcgcatg gtgcgtacgc
    22201 atctatctga tgtgatcgga aagcgggcag acctgcgtat ttcggatcgc gtttttggtc
    22261 atgcgctacg gctgaaaaac aacgtgcgat cgaaatccac cggatcgttt atctcgcaga
    22321 tccgcgaact ggaatcagtg cgggagctta ttacgtccac caccatcggc gcggtggcgg
    22381 acctgccatt cttcctgctg tttgtcttta ttttgtggat gatcggcggc tggctggtgc
    22441 tggtggtttt gctggcgctg ccgttgctgg tgatccccgg cctgctggtg caacgcccgc
    22501 tggcgcggct ggcgaacgaa ggaatgcgcg agtcagcggt acgcaacgct acgctggtgg
    22561 aagccgtgca gtcgattgaa gatattaaac tgctgcgcgc cgaacagcgt tttcagaatc
    22621 agtggaacca caccaacgac gtggcctcgt ccatcagcat gaagcaacgt tttctcaccg
    22681 ggctgctgct cacctggacc caggaagtgc agtccatcgt ctacgtggtg gtgctgctgg
    22741 tgggctgctt tatggtgatg aacggcgaca tgaccaccgg cgcgctggtc gggacgacga
    22801 tcctggcttc gcgcaccatt gcgccgctgt cgcagatatc cggcgtgctc tcgcgctggc
    22861 agcaggcgaa agtagcacgt aacggccttg atgaactgat gaagcgcccg gtggatcagc
    22921 cggaacacgg caaactggtg cataaagcgg tgctgcacgg taattatcag ttcagcaacg
    22981 cggtgtttta ttacgacgaa gaagagaaga ttgccgatgt agcgattggc aaactcaaca
    23041 ttcaggctgg tgagaaaatc gccattctcg ggcgcaacgg cgcgggtaaa tcgacgctac
    23101 tgcaaatgct cgccggaatg cgtatcgccc agcaggggca ggtgttgctg gataacatca
    23161 gtatcgggca gcttgatccg gcagatctgc ggcgagacat ggggctactc agccagaccg
    23221 ggcgactgtt ctttggttcg ctgcgggaaa acctcaccat ggggatgccg gaagccagcg
    23281 atgaagatat cgaacgcgcg ttaaccctca gcggagcgct gccctttgtg cagaagcaga
    23341 aaaacggcct caactacatg attcaggaag ggggatttgg cctctctggt ggtcagcggc
    23401 aaacgctgct gctggcgcgg ctactcatct ctcagccgaa tatcgtcctg ctcgacgaac
    23461 ccagcgcctc actggacgaa atggcagaag cgtatttaat cgaacaactg aaacagtgga
    23521 ttgggcatcg cacgctgatt atcgccactc accgcacggc gatgctgcaa ctggtggata
    23581 ggataatcgt gatggatcag gggcggattg tgatggacgg cgcgaaagag gccattttgc
    23641 gtgaacaggg cgagcccaca gcgcgacggg tggtgttgca ggagaagaat aaggggtctg
    23701 cggcatgaac ggacaactgc aatataaccg tggtctgact gaaccccgcc tgccgcgttc
    23761 ggcgctggcg gtacgcgtta ccgctgtgat gctgctctgt tttctcggct gggcgtggta
    23821 cttccagctt gatgaagtca ccaccggcag cggcacggta gaaccttccg ggcgcgaaca
    23881 ggtggtgcag tcgctggaag gcggaattct gtatcatctc gacgtgaaag ttggcgatat
    23941 tgtcgaacag ggccagccgc tggcgcagct taaccgcacc aaaacggaat ccgacgtcca
    24001 ggaagcgatg tcgcggctgt acgcggcgct ggcaacctcg gcgcggctgc gggcggaagt
    24061 gagcaataaa ccgctggtct tccctgacga actgaataaa ttccccgaac ttatcgagtc
    24121 ggaaacggcg ttgtacaaca ctcgccgcga cgggttgaac aaagccacca ccgggctgac
    24181 gcaggggatt agcctggtca atcgcgaact ggcgatgacc cagccgctgg taaaacaggg
    24241 ggcagcgagc agcgtcgaag tactgcgcct gcaacgccag gccaatgaac tggaaaacaa
    24301 actcagcgac gtgcgcacgc aatactacgt tcaggcgcgc gaagagttag caaaagccaa
    24361 tgcggaagtg gaaacccagc gttcggtgat ccgtggacgg gaagactctc tcaccaggct
    24421 caactttacc gcgccggtgc gaggcattgt tcaggatatt gatgtcacca cggttggcgg
    24481 cgtcatcgcc cccggtggta aactgatgac catcgtgccg ctggatgagc aactgctgat
    24541 tgaggcaaaa atctccccgc gcgatgtggc gtttatccac ccggggcaaa aatcgctggt
    24601 gaaaataact gcctacgatt actccatcta cggcggcctg ccgggagaag tggcggtgat
    24661 atcaccagac accgtgcagg atgaagtgcg gcgcgatgtt tattactatc gggtgtacat
    24721 tcgcactttc agcaaccatc tggaaaacaa aagcaaacag cagtttccga tattccccgg
    24781 tatggtggcg acggtcgata tccgcaccgg caaaaaaagc gtgctggatt atctgttgaa
    24841 accctttaac aaagcacagg aggcgttgcg cgaacgatga acattagcga tgtagcaaaa
    24901 attaccggcc tgaccagcaa agccattcgc ttctatgaag agaaggggct ggtgacgccg
    24961 ccgatgcgca gcgaaaatgg ttatcgaacc tacacgcagc agcatctcaa cgaattgacc
    25021 ttactgcgcc aggcgcgcca ggtgggcttt aacctggaag agagcggcga gctggtgaat
    25081 ctgtttaacg acccgcagcg tcacagtgcc gacgtcaaac gtcgcacgct ggagaaggtg
    25141 gcggagattg aacggcatat cgaagaactg caatctatgc gcaaccagct actggcgctg
    25201 gcgaatgcct gccccggtga tgacagcgcc gactgcccga ttatcgaaaa tctctccggc
    25261 tgctgtcatc atcgggcggg gtgaaaatca gtgaatcccc gtttgcaccg aaagaatatg
    25321 ctgcaaactt tctaccataa tgcgccccga ctgaaataag ccttcctgga tttcgttgtt
    25381 aacattcaca gcgtgaacca gggaggctaa taatactact tgcaagaagg caaaaataaa
    25441 cgcaccgata atcataatat gcgtcagggg tattttcctt tgctccttca ttttaaataa
    25501 cccacaaact ggccttcttt cattaaggcg acttttcctg tgtgatgata aacaatgacg
    25561 ttatatagac ctgactgata aacagtggca tctttattgt cttcatcaat tgcacataat
    25621 gaatcattga gataagcaaa gtttttactg ctcattctaa aggtcatgcc aaatccttta
    25681 taaatggctt tctttgccgc ctgggcgtgt tgtgcggctt ctgcattttt aaatgcctgt
    25741 gcctcggatg cacttaaaat agccgcctga cgctgctgtt ctgcgaggta acaggtgtca
    25801 tgactgacgc cctgcgcctg gcaatctgcg atgcgggaag tgggtgaact acagccagcc
    25861 agtaatataa ccgccagcag ggaaaacgat ttaatggagc tattcatcat tgttaacctt
    25921 aaataatgta caaattaatg aagaggtgaa cccggcaagc ttccttgctc ttatttatat
    25981 tgaggattga aatattagaa cgaatatccc acgccgacac ctgcgccgaa atcagattgt
    26041 gtatccgcag aaataccggc tttaagtacc gtatgcgttc cggcattaaa tgatgttccg
    26101 acagacaccg cagattcacc gttgaaggtt cccgctcccg ctgacaccat catcacgtca
    26161 ccggttttaa cctgtggttg tgaggcgata gcgatggcag atgcagttcc cgcattggct
    26221 tctttacgat tgtcatcaac ttcgtttttt agcgaactga aatggttatt gagcgtgtta
    26281 atttgctggc tgttatctgc aacccgtttt tgggtgttgg ttaactgttg gtgcgtttgg
    26341 gttacgtctt gtgacaggct gtcaatttgt tcatgtgcct gggcagactc ctgcgccaga
    26401 ctttgcattt gtttatcgta ttgagtcctt tcattttgct gttgtactgc cacatcggtt
    26461 gtcagcgcat tcattttctg cccttgttca gtggcggtgc gctgattagc ctcattttga
    26521 gccgccagtc tggcattaat catttcctga taatgcgcgg tattttgcgt atctccagtc
    26581 gcggcgagcg ttttttgtgt ggctacgatc tgcgcctgtt gctctgtaat ttgcgtttgc
    26641 tgggcgtcag ttaccgcaga ctgtcgggcg ttgatcatgc tctgataacg ggcagttgtc
    26701 tgtacatcac ctgtagcggc aacggttttt tgtcctgcgt caggatctgt ctggacagtg
    26761 ggagtctgag tggtatcagg tgtttcagaa gggatgtagt gatgttcagt caaataggta
    26821 tacgcagcgg ggctgttgtc tgtgcgttct aaaaagcggg taatcttctg ctcaatctcc
    26881 gattgcgaca tgccagagtt aatggctgaa agctctgttg ccgccgcttc aatggtatca
    26941 ccagctaaaa caacagggct ggatgttgct gaaattatga gtgccagtaa agctacgttt
    27001 acagttttca ttcattttct cttgttcaaa ataagtcgta ttaatgtttc tataagaaac
    27061 aaataatatt gttttaagaa ataaactgcg ttgcaattta atcgaaatct acgccgccat
    27121 ttgtgtgatg taaagcgcat ttttgtctgt tgtaagcgta aacaccatca ataagaaaca
    27181 aaaatggttt cttatataaa taaaaaagga aatcatttcg tgctctttca atgtaaaaaa
    27241 gagtaaagac ttgttttgat aagcgaaata aattctgttt ctggactaaa cgtgaagcgg
    27301 ttcctgatgt gtgctaaaat gcgtttcttt gataaatctc aatttttaag aggtttacgc
    27361 caggcaggaa aatttaaatg aatgaatttg agaagatttt taatgaaatg aatctggacc
    27421 gggcattgct accgatcctt tttcgttcga accggtcgac agtgtggaag tatttaagcg
    27481 gcgatagtac ggctcctgcc tctgccatgt cgctgattat gctgttgcag ttgatacaga
    27541 aacgaaaccc cgatctgtta gccgaatggt taacgctttc tgatttcacc attccgccag
    27601 aagtttacct ggaccagcct gattactgga aagggtgggt gtatacgcaa cacaaggtca
    27661 ataaaaacgt cctggaatat ctaaaaaaac actatccgga tgaagaccaa aaaagtatga
    27721 gtaaaggccg ggaagagtaa attgccaatg ccagttaaca tttaacatgg cggaggcggc
    27781 ctacggtttt ggcgtgatat gacgcgcccg cgtcgcatca ggcgtcggtg cacgactgcc
    27841 ggatgcggcg taaacgcctt atccggccta cgattcgggc gcagacttcc gggcatgata
    27901 agacgcgtca gcgtcgcatc aggcgatgac cgcacggatg atcagcgtta tcccttctat
    27961 cgcaatgact tcaacatgcg tacctgcgcc gagatcctcg ctggcgctga caggccatga
    28021 actgtcaccg acgcgcatat gaccgcgccc gttgaccagc ggagattcca gcacaaaacg
    28081 tcggccaatc agctgctgcc cgcgctggtt taaatgactg tcgctgtgct tttgttcgcg
    28141 cacccgccgc gacaaccatt tccaccacag ccaggcggcg agcagcgtca ggacggcaaa
    28201 catcacccct tgccactccc aacccagcgg caccagccag accaccaggc cagtaatcac
    28261 tgctgccacg ccactccaca acaaataacc atttccgccc agcatctcgg ctgccagcag
    28321 caaaccgccg agactgagcc agaaaatatg tggatgaacg actattaact ccatcatggc
    28381 tgagtccgct tgttggcgct gtctttcacc agctcggcaa tcccggcaat cgaccccatc
    28441 aggctgctgg cctctaatgg catcatcact actttgctgt tactggagga accgatctgc
    28501 tgtaacgctt cggtgtattt ctgcgctacg aagtagttca ccgcctgaat atcaccggag
    28561 gcgatggctt cagacaccat tttggtggcg cgggcttctg cttcagcgga acgttcacgc
    28621 gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat ttgcgactgt
    28681 ttttcacctt cggctttgag gatttccgcc tgacggatcc cttccgcttc aagaatgtaa
    28741 gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg aagagataag ctctgccggt
    28801 gggcgcacgt cgcgaatttc aatacgggtg actttaatcc cccacgggtt ggtggcctca
    28861 tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt
    28921 tccattgaac ccaacacggt acggatgtta gtcatggtca ggttgatgat cgccagctcc
    28981 agattgctga cttcataagc cgcgcgtggc gcgtcaatca cctgaataaa acagactgcg
    29041 tcgatggtaa cgttggcgtt atctttcgag ataacttcct gggaagggat atcgagcact
    29101 tgctccatca tattgatctt gcgaccaatg cgatccataa acggcaccac cagactgagc
    29161 cccggctgta acgttttggt atagcgacca aaacgttcta ccgtccattg atagccctgc
    29221 ggcacgattt tgacgcccgc gccgacaatg accagcgcga caaaaatgag aatcgggata
    29281 aagataagca tcggaaaaac ctcctgttgt accgtccata atcagcaaaa ttgctgcttg
    29341 attaaacaaa ttatacctga ttactgaaag agagttcccc cttattcctg cgaaggataa
    29401 actgttttta gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag tcctttgctt
    29461 cagctacaaa acgtaggata tctggcgggt gatgcgaaga ttcttaataa catcaatttt
    29521 tcgctgcgtg ctggcgaatt taagttaatt accggtcctt ctggttgtgg caaaagtacg
    29581 ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg gaacgttact gtttgaaggt
    29641 gaggatgtca gcacactaaa gccagaaatc taccgtcagc aagtctctta ctgcgcccag
    29701 acgccgacgc tgtttggcga tacggtatac gataatctga tctttccctg gcagatccgt
    29761 aaccagcagc ctgacccggc catttttctc gattttcttg aacgcttcgc cttgccggac
    29821 agcattttga cgaagaatat cgccgagcta tctggtggtg aaaaacaacg catctcattg
    29881 attcgtaacc tgcaatttat gccgaaggtt ttattgctgg atgaaatcac cagtgcgctg
    29941 gatgaaagta ataaacataa cgttaatgag atgatccatc gttatgtgcg cgagcaaaat
    30001 attgccgtgc tgtgggtgac acacgataaa gacgaaatta atcatgcgga taaagtgatt
    30061 acactgcaac cgcatgccgg agaaatgcag gaagcacgct atgaactcgc ataatattac
    30121 taacgaatca ttagcactgg cattaatgct ggtagtggtg gcaatcttaa ttagccataa
    30181 agaaaaactg gcgctggaga aagatattct ctggagcgtc gggcgcgcga taattcagct
    30241 gattattgtt ggctatgtgc tgaagtatat tttcagcgtg gatgatgcca gcctgacatt
    30301 attgatggtg ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata
    30361 tattgctaaa gcttttatct catcgtttat tgccattacg gtcggggcgg gaattaccct
    30421 ggcggtgctg attctctcag ggtcgattga atttatcccg atgcaggtga tccctatcgc
    30481 cgggatgatt gccggtaacg ccatggtagc ggtggggttg tgttacaaca atttagggca
    30541 acgggtcatt agcgaacagc aacagattca ggagaaactg agtcttggcg cgacgccgaa
    30601 gcaggcttca gcgatattga ttcgcgacag tattcgcgcg gctttaattc cgacggtcga
    30661 ttcagcaaaa acggttggct tagtgagttt accaggaatg atgtccgggc tgatatttgc
    30721 cgggattgat ccggtgaagg cgattaaata tcagattatg gtgaccttta tgctgctctc
    30781 aaccgccagc ttgtcgacca taattgcctg ctatttaacc tatcgtaagt tttataattc
    30841 gcgccaccag ttggtggtga cgcaattgaa gaagaaatga tgcgatgccg gatgcgcagc
    30901 atcgcatccg gcgttgtggt tcatgtgccg atcaacggaa tatcaataca acaatgcata
    30961 cagctggcgg cgatacttcg acgccagtgc atcacccgta cccagcgcag cgaggatctc
    31021 ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa
    31081 cagcaactcc agcgcctctt cattgcgccc aacctgatgc agttgcagcg ccagttgcgt
    31141 cgccagtgcg gcatcttctg gattctccgc cacctgctgt tgcaactgtt gaatttccgg
    31201 cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc agcccctggt agcgggtgtc
    31261 ctgatcctgc aacggaatgg ttttcagcac cgcttccgca tcttcagaac ggttcagcgc
    31321 aatcagcgtt tctgccagca gcaggccgat ctccccgttc tgattcgaca actgccaggc
    31381 gtctttcagc aatggcaggg catcggtgta attgccttcc tgcatcagtt gcatcgcctg
    31441 ctgcgctttc agctcttctt cgcgcggcag cactttatcc agcagggcgc ggatcgcctc
    31501 ttccggttgc ggcccctgga agccatctac cggttgcccg ttctggaaca gatacacggt
    31561 cggaatcgcg cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc agtccagctt
    31621 cgccagaata aattgcccgt tgtactgcgc cgcaaggctt tccagaattg gggttaactg
    31681 caaacagtgc tggctacgtt cagaccaaaa atagaacagc accggagtgg tcatcgactg
    31741 ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca atattttcta cggacatgga
    31801 gtcgctctct gttgtcgata ttttctttga catgggggct taagcgcgcg cttcaactca
    31861 cccctgcaat attttgtcca tcacgcgccc cggcagcagg cgcttaagca ccattaccgc
    31921 ccaggtcacc agcgttaccg gatagcgcat cttcggcttc tcgctaataa aagcatggcg
    31981 tactttgtcc accaccgctt ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc
    32041 gactggttta tcactttgcg tctggttgac gttgtcggtg aagcgagtac gaatgggacc
    32101 gggttcgatc aggctgactt taattccgct gcggcgcagc tccatgcgca gcgcgtctga
    32161 ccacgcctcc agcgcatatt tactggccgc gtaagcgcca cgacccggtg tggagattaa
    32221 tcccatcacc gatgaggtca tcacaatacg cccttcaccg tgcggcaaca tcgcgggtaa
    32281 caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg gaaaactgct gttccatctg
    32341 cgcacggctg atggtggaaa gggggccata catgccgaat ccggcattgt taaagatccc
    32401 atacagacaa ttatcggtca gggcgatcac ctcgtcggct gcgcgatcaa cactttctgg
    32461 tgaatccaga tcgatcaaca cgccggtaaa tcccatgctg ttcatgcgct caacatcatc
    32521 cggtttccgg caacctgcca gcacatgaaa accctggcgt tttaattcga gcgcgctttc
    32581 caggccaatt ccactggaac atccggtaat taagaccgat ttttgcataa ctttacctgt
    32641 caggatctcc gttgctttat gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc
    32701 agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt
    32761 gtggcttgag gtagacctct tccattaaaa agggcagcag cggaacatca aactctttgg
    32821 cgagtttggg gtaaatggcg ctaaaggctt cattataacg gcgaccatag tttgcaggca
    32881 gacgtatttg cattaacaat ggttcagcgt tggcggcttt gacatcctgc aaaatctggc
    32941 gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa accgtcattg ccgcccagtt
    33001 caaccagcac ccaacgcggc tgatgctgtt tcagcagagc cggaaggcgc gccagccctt
    33061 gttgcgaggt gtcgccgctg atgctggcat tgactaccga cgttttactc tgccacttat
    33121 cattcaacaa ggcaggccag gccgcgctgg cagacattcg atacccggcg ctcaggctat
    33181 cacccagaat caataacgtg tccgctgcgg cagcacggaa ggttaacagg accagaaaca
    33241 ggaagggcaa atgccagcgg aaaacattgt tgaagttcat catcttaaga agtccgtcgg
    33301 tcagggggag catgaactct ccatcctcac cggagttgag ctggttgtca aacgtggcga
    33361 gaccatcgca ctggtgggcg agtcgggatc gggtaagtca accttgctgg cgatcctcgc
    33421 cgggctggat gacggcagca gtggcgaagt gagtctggtg ggacaaccgc tacataatat
    33481 ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt
    33541 tatgttaatt cctaccctta acgcgctgga aaacgtcgag cttccggcgc tgctgcgcgg
    33601 tgagagtagc gcggaaagtc gtaacggagc gaaagcgttg ctcgaacagt tagggctggg
    33661 taaacgtctg gatcatcttc cggcacagct ttccggcggt gaacagcaac gagtggcgct
    33721 ggcacgagcc tttaacggtc gacctgatgt gctgtttgcc gacgaaccca ccggtaacct
    33781 tgaccgccag acgggcgata aaattgccga cctgctgttt tccctcaacc gtgagcatgg
    33841 caccacgttg attatggtga cccacgacct gcaactggcg gcacgctgcg accgctgctt
    33901 acggctggtg aacgggcagt tgcaggagga agcatgattg cacgctggtt ctggcgcgaa
    33961 tggcgctcgc cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg
    34021 ctggcgctgg gcaatatcag cgatcgcatg gagaagggct taagccaaca aagccgggag
    34081 tttatggcgg gcgatcgggc gttgcgcagt tcgcgcgaag tgccgcaagc gtggctggag
    34141 gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga ctttcgccac catgacattt
    34201 gcaggcgaca caccgcagct ggcgaacgtt aaagcggtgg atgatatcta cccgatgtat
    34261 ggcgatctgc aaaccaatcc ccctggcctg aaaccgcagg cgggcagcgt attgctggcc
    34321 ccacgcctga tggcactgct taacctgaaa acgggcgaca ccattgacgt gggcgatgcc
    34381 accttgcgga ttgccggaga agtgattcag gaaccggatt ccggttttaa ccccttccag
    34441 atggctccgc gtctgatgat gaatctggcg gatgtcgata aaaccggagc cgtgcagccg
    34501 gggagtcggg tcacctggcg ctataaattc ggcggcagcg agaaccagct cgacagctat
    34561 gagaaatggt tgttacctca gcttaaaccc gaacaacgct ggtacggtct ggaacaggac
    34621 gaaggcgcgc tggggcgatc gatggaacgc tcgcaacagt tcctgctgct ttcggcactt
    34681 ctgaccttgc tgctggcagt ggcagcagtg gcggtagcga tgaatcatta ctgtcgcagt
    34741 cgctacgatc tggtggcgat cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag
    34801 ctaatcgtcg gtcagtggtt gatggtgctg acgctttcag ccgttaccgg tggggcgata
    34861 ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta
    34921 ccgccagcca gcctctggcc atggctatgg gcgctgggca ccatgacggt catctcgctg
    34981 ctggtggggc tacgaccata ccgcttgttg ctggcaacgc agcctttacg cgtattacgt
    35041 aattatgtgg ttgcgaacgt ctggccgctg aagttttatc tgccgattgt cagtgtggtg
    35101 gttgtgctgc tgctcgccgg attaatgggt ggcagcatgc tgctttgggc ggtgctggcg
    35161 ggcgcggtag tactggcttt gctgtgcggt gtgctgggct ggatgctgct gaatgtactt
    35221 cgccgcatga cgctgaaatc gctgcctctg cgcctggcgg ttagccgcct gttacgtcag
    35281 ccgtggtcaa cgttaagcca gctttcggca ttttcgctct cctttatgct gctggcactg
    35341 ctactggtgc tgcgtggcga tctgctcgac cgctggcagc agcagttacc gccagaaagc
    35401 ccgaactact ttttaatcaa catcgccaca gaacaggtta cgccgctaaa agcgttcctc
    35461 gcggaacatc agatagtccc ggaatcgttt tatccggtgg tgcgagcgcg gctgacggcg
    35521 attaacggca agccgacaga aggcaatgaa gatgaggcgc tcaaccgcga actcaatctt
    35581 acctggcaaa atacgcggcc cgatcataat ccgattgtcg ccggtaactg gccgccaaaa
    35641 gctgatgaag tttcgatgga agaggggctg gcgaaacgct taaacgttac cctcggcgat
    35701 accgtgactt ttatgggcga tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa
    35761 gtggactggg aaagtctgcg gcctaatttc tattttattt tccctgaagg ggcattagac
    35821 gggcaaccgc agagctggct taccagtttc cgctgggaga atggcaacgg catgttgacg
    35881 caactcaacc gccagttccc taccattagc ctgttagaca ttggcgcgat tttaaaacag
    35941 gtcggtcagg tgctggagca ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc
    36001 gcctgcggta tgttgctgtt gctggcacag gtgcaggtgg gaatgcgtca gcgtcatcag
    36061 gagctggtgg tgtggcgcac actcggtgcg gggaaaaaac tgctgcgtac cacgttgtgg
    36121 tgtgagttcg ccatgcttgg gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg
    36181 gcactggcag tgttgcaggc gaaagtgttt gattttccgt gggagccaga ctggcgattg
    36241 tggattgttc tgccgtgcag cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggc
    36301 gcgagactgg ttaagggtaa ggcgctgttc aggcagtttg cggggtgatg agagtaaata
    36361 aggggcgttc tggttgaatg gaacgccttg tttaaattaa ttctacaata tgtttcattg
    36421 atgatgttat tgaattggtg attt
//
LOCUS       DAZKIF010000034        35906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZKIF010000034 DAZKIF010000000
VERSION     DAZKIF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.034"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(85..171)
                     /locus_tag="ACHOTW_001707"
     tRNA            complement(85..171)
                     /locus_tag="ACHOTW_001707"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(135..137),aa:Leu,seq:cag)
     gene            complement(192..267)
                     /locus_tag="ACHOTW_001708"
     tRNA            complement(192..267)
                     /locus_tag="ACHOTW_001708"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(232..234),aa:His,seq:gtg)
     gene            complement(326..402)
                     /locus_tag="ACHOTW_001709"
     tRNA            complement(326..402)
                     /locus_tag="ACHOTW_001709"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(366..368),aa:Arg,seq:ccg)
     gene            complement(505..1890)
                     /gene="yifK"
                     /locus_tag="ACHOTW_001710"
     CDS             complement(505..1890)
                     /gene="yifK"
                     /locus_tag="ACHOTW_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9843835.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(2081..2821)
                     /gene="wecG"
                     /locus_tag="ACHOTW_001711"
     CDS             complement(2081..2821)
                     /gene="wecG"
                     /locus_tag="ACHOTW_001711"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP9843836.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2824..4176)
                     /gene="wzyE"
                     /locus_tag="ACHOTW_001712"
     CDS             complement(2824..4176)
                     /gene="wzyE"
                     /locus_tag="ACHOTW_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP9843837.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4173..5252)
                     /gene="rffT"
                     /locus_tag="ACHOTW_001713"
                     /gene_synonym="wecF"
     CDS             complement(4173..5252)
                     /gene="rffT"
                     /locus_tag="ACHOTW_001713"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP9843838.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCALGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5249..6499)
                     /gene="wzxE"
                     /locus_tag="ACHOTW_001714"
     CDS             complement(5249..6499)
                     /gene="wzxE"
                     /locus_tag="ACHOTW_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP9843839.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6501..7631)
                     /gene="rffA"
                     /locus_tag="ACHOTW_001715"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6501..7631)
                     /gene="rffA"
                     /locus_tag="ACHOTW_001715"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP9843840.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7636..8310)
                     /gene="rffC"
                     /locus_tag="ACHOTW_001716"
                     /gene_synonym="wecD"
     CDS             complement(7636..8310)
                     /gene="rffC"
                     /locus_tag="ACHOTW_001716"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP9843841.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8288..9169)
                     /gene="rfbA"
                     /locus_tag="ACHOTW_001717"
     CDS             complement(8288..9169)
                     /gene="rfbA"
                     /locus_tag="ACHOTW_001717"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9843842.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9188..10255)
                     /gene="rffG"
                     /locus_tag="ACHOTW_001718"
     CDS             complement(9188..10255)
                     /gene="rffG"
                     /locus_tag="ACHOTW_001718"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9843843.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(10255..11517)
                     /gene="wecC"
                     /locus_tag="ACHOTW_001719"
     CDS             complement(10255..11517)
                     /gene="wecC"
                     /locus_tag="ACHOTW_001719"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP9843844.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11514..12644)
                     /gene="wecB"
                     /locus_tag="ACHOTW_001720"
     CDS             complement(11514..12644)
                     /gene="wecB"
                     /locus_tag="ACHOTW_001720"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP9843845.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12700..13746)
                     /gene="wzzE"
                     /locus_tag="ACHOTW_001721"
     CDS             complement(12700..13746)
                     /gene="wzzE"
                     /locus_tag="ACHOTW_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP9843846.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13758..14861)
                     /gene="wecA"
                     /locus_tag="ACHOTW_001722"
     CDS             complement(13758..14861)
                     /gene="wecA"
                     /locus_tag="ACHOTW_001722"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP9843847.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(15101..16360)
                     /gene="rho"
                     /locus_tag="ACHOTW_001723"
     CDS             complement(15101..16360)
                     /gene="rho"
                     /locus_tag="ACHOTW_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9843848.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16445..16546)
                     /gene="rhoL"
                     /locus_tag="ACHOTW_001724"
     CDS             complement(16445..16546)
                     /gene="rhoL"
                     /locus_tag="ACHOTW_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP9843849.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16687..17016)
                     /gene="trxA"
                     /locus_tag="ACHOTW_001725"
     CDS             complement(16687..17016)
                     /gene="trxA"
                     /locus_tag="ACHOTW_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP9843850.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            17147..18412
                     /gene="rhlB"
                     /locus_tag="ACHOTW_001726"
     CDS             17147..18412
                     /gene="rhlB"
                     /locus_tag="ACHOTW_001726"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP9843851.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGANGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18548..20032
                     /gene="gppA"
                     /locus_tag="ACHOTW_001727"
     CDS             18548..20032
                     /gene="gppA"
                     /locus_tag="ACHOTW_001727"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP9843852.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(20079..22100)
                     /gene="rep"
                     /locus_tag="ACHOTW_001728"
     CDS             complement(20079..22100)
                     /gene="rep"
                     /locus_tag="ACHOTW_001728"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP9843853.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22317..22766
                     /locus_tag="ACHOTW_001729"
     CDS             22317..22766
                     /locus_tag="ACHOTW_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9843854.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22965..23246
                     /gene="ppiC"
                     /locus_tag="ACHOTW_001730"
     CDS             22965..23246
                     /gene="ppiC"
                     /locus_tag="ACHOTW_001730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP9843855.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23292..24767)
                     /gene="ilvC"
                     /locus_tag="ACHOTW_001731"
     CDS             complement(23292..24767)
                     /gene="ilvC"
                     /locus_tag="ACHOTW_001731"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP9843856.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24917..25810
                     /gene="ilvY"
                     /locus_tag="ACHOTW_001732"
     CDS             24917..25810
                     /gene="ilvY"
                     /locus_tag="ACHOTW_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP9843857.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(25862..27406)
                     /gene="ilvA"
                     /locus_tag="ACHOTW_001733"
     CDS             complement(25862..27406)
                     /gene="ilvA"
                     /locus_tag="ACHOTW_001733"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP9843858.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27409..29259)
                     /gene="ilvD"
                     /locus_tag="ACHOTW_001734"
     CDS             complement(27409..29259)
                     /gene="ilvD"
                     /locus_tag="ACHOTW_001734"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP9843859.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29324..30253)
                     /gene="ilvE"
                     /locus_tag="ACHOTW_001735"
     CDS             complement(29324..30253)
                     /gene="ilvE"
                     /locus_tag="ACHOTW_001735"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP9843860.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30273..30536)
                     /gene="ilvM"
                     /locus_tag="ACHOTW_001736"
     CDS             complement(30273..30536)
                     /gene="ilvM"
                     /locus_tag="ACHOTW_001736"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP9843861.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30533..32179)
                     /gene="ilvG"
                     /locus_tag="ACHOTW_001737"
     CDS             complement(30533..32179)
                     /gene="ilvG"
                     /locus_tag="ACHOTW_001737"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP9843862.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32182..32232)
                     /gene="ilvX"
                     /locus_tag="ACHOTW_001738"
     CDS             complement(32182..32232)
                     /gene="ilvX"
                     /locus_tag="ACHOTW_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP9843863.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(32319..32417)
                     /gene="ilvL"
                     /locus_tag="ACHOTW_001739"
     CDS             complement(32319..32417)
                     /gene="ilvL"
                     /locus_tag="ACHOTW_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP9843864.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32770..34290
                     /gene="yifB"
                     /locus_tag="ACHOTW_001740"
     CDS             32770..34290
                     /gene="yifB"
                     /locus_tag="ACHOTW_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP9843865.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(34315..34653)
                     /gene="maoP"
                     /locus_tag="ACHOTW_001741"
     CDS             complement(34315..34653)
                     /gene="maoP"
                     /locus_tag="ACHOTW_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP9843866.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34772..35611
                     /gene="hdfR"
                     /locus_tag="ACHOTW_001742"
     CDS             34772..35611
                     /gene="hdfR"
                     /locus_tag="ACHOTW_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP9843867.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(35707..35782)
                     /locus_tag="ACHOTW_001743"
     tRNA            complement(35707..35782)
                     /locus_tag="ACHOTW_001743"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35747..35749),aa:Trp,seq:cca)
     gene            complement(35791..35867)
                     /locus_tag="ACHOTW_001744"
     tRNA            complement(35791..35867)
                     /locus_tag="ACHOTW_001744"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35831..35833),aa:Asp,seq:gtc)
BASE COUNT         8760 a         9800 c         9064 g         8282 t
ORIGIN      
        1 tggtcccaaa ccagttgcgc taccaagctg cgctactcgc cgaaatactg ctttttgaat
       61 ttttagttca attctttaaa gtcgtggtgc gagggggggg acttgaaccc ccacgtccgt
      121 aaggacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg cattgtcaca
      181 acttctaata atggggtggc taatgggatt cgaacccacg acaactggaa tcacaatcca
      241 gggctctacc aactgagcta tagccaccac tacaaatctt gttacgcggt attactacca
      301 ccgcagctca agcgccggga ctaaatggcg cgcccgacag gattcgaacc tgagacctct
      361 gcctccggag ggcagcgctc tatccagctg agctacgggc gcttagcgcc gttgcggggg
      421 tggataatac ggacttcccc cccctctgtc tagtcccttt ttaaataaat tgcgcgtttg
      481 gttacggttt gtgcgttttg ctgcttattc ctccagttta tgcgctttcc cgtggcgatt
      541 aaggccaaaa actttataaa tcgccgtcac cgccagcata aagatgatgc caacaaacag
      601 cgacatacgc gtatcttcat taaagtacat gccgatcaaa acgcaaatca ggaatgccat
      661 tgttacgtag ttggcccacg ggaacaggat tgagcggaac ggatggctgg caatcgccgc
      721 tttatgtgca cgccgaaaac gcagctggct tatcaatatc acaaaccatg gcaccatccc
      781 cggaagcacg ctggcactgt agacgtagac aaacacacgc tgcggattgg gaatgatgta
      841 gttcaggcat gagccaatta gcagaatagc aatagatact gccacacccg caaccggtac
      901 gccgtgacgg gaaactttcg ccatcgccgc cggtaactga cggtttttcg ccagtgcgta
      961 gagcatacgt ccgcaactgt acatgccgct gttacagcca gagagcgcag ccgtcagcac
     1021 cacaaagttg ataatgcccg ccgctgcggt aataccgatt ttggcaaaag tcagtacgaa
     1081 cgggctgccg ttgctgccta tttcattcca cgggaagatg gtgacgataa cgaaaatcgc
     1141 gccaacgtag aaaatcagga tccgccacag caccttgcct acggcactgc gcagcgtcac
     1201 ctgcggattc ttcgcttcac cggcagtaat gccaatcagc tccacgccct ggtaggacgc
     1261 caccacaata cacagcgcgg tcaggaaccc tttccagcca cccgcaaaga aaccgccatg
     1321 ctctgtgaga ttgctaaaac caatcgactg cccgccattg ccaaagccaa agaaaatcac
     1381 gcccaggcca atgacaatca tcacgataat cgtggtgact ttgatcatcg cgaaccagaa
     1441 ctcgatttcg ccgtacaacc gcaccgctgc cagattcgcc aacgccacca gcgccactgc
     1501 gatcaatgcg ggtatccact gcgccatctc cgggaaccag aactggacat aaacgccaat
     1561 ggcggtgatt tcagagatcc ccaccgccat ccacataaac cagtaagacc aggcggtgag
     1621 atagccaaag aacgggctca tataacgatg cgcataaacg gcgaacgaac cggtaaccgg
     1681 ttcgaggaac aacatttcgc ccattgaacg catgatgaaa aagacgaaca gcccggcgat
     1741 gatataggcc aacaatacgg atggcccggc ccatttcagg gtactggcgg cccccataaa
     1801 caggccgacg ccaatggtgc cccccagggc gatgagttcg atatgtcgag cttccagccc
     1861 acgctgtagc tctggtttgt tatctgccat aaatcctcgt gttgtgtttg catgctttcc
     1921 ggtgttaccg gttatcgtta tgggtacatc gagtgttgcg aatgttttcg taattcagga
     1981 gaaatggcaa ataaagcatt aaaaatttga atgctttgtg taataaaaaa gcagacaggc
     2041 gacggagtga ccactccgtc gctttacaaa gagaggaaaa tcagaggttg ccggtgtaat
     2101 gccagcgtaa ataacgcagc aaacgaagct gacgcttaat gcggctcggc tgcgaaagca
     2161 ggcggtagag ccactccagc cccagcgttt gccagatttt cggtgcgcgt tttacgtgac
     2221 cggtgaaaac atcgtaagtc ccgccaacgc ccatatacag cgcatctgga tgcaccagac
     2281 ggcagtcgcg catgaagatc tcctgctttg gcgatcccat cgcaacggtg acgatttgcg
     2341 ctccgctggc atgaatgcgt tcaaacagcg cctgacgctg ctcgggttta aaataaccat
     2401 cctgactgcc aacgatattc acattccact ggttgcgcag tttagcttca gtttgcgcca
     2461 gcacttcagg tttaccgccc acaagaaata ccgtcgtccc ttctttgcct gcgcgcgcca
     2521 tcagctcttc ccagagatcg gcaccggcaa cgcgggaaac ctgcgcctgc gggtactttt
     2581 tacgtactga acgtacaacg ctgatgccat ccgcatattt aaattcggca gcgttaatta
     2641 actccctgac ctcggcgttc tcttcaatgg tcagcatttt ttcagcatta atggcaacca
     2701 gcgttccctg cttaagctgc ccgtcagcaa acagataatc gagggcatgc tgcatatcac
     2761 gccaaccaat caactgtaag ccacgcagcg tataggtcgg tgccgtggtg ttgttattca
     2821 ttgttatcct tcaacctgcg tccggagcga tgattttgta cgtttatgaa tgagtccggc
     2881 gctttcaaaa agccagtaca acagttttgc gatcatcaga catgcgccga agaccacgat
     2941 aaaaaagacc acgcgtgaga caaacgaatc cagcccttca cgcgccagca cgatcatatt
     3001 gaagatcgcc ccaaagcaga aactgtgcaa tatcgcagcc ttatagcgat tagtttcgcg
     3061 gttgcccagc tcatacagcc agtcgaacca tttgatgatc agtccaacca caatcgcccc
     3121 gagcgggatg aacaacgcgc cgcccatcac caccagtgag cctataagcg taggcgagat
     3181 cgccagtccg gagtggttat tcagcacttc ccaggtaaag tagttggctg agttcagcac
     3241 catactcggg cgacccggcc acagccagga agggataaag acatagaaat cgcggacaat
     3301 tggcgccagg ccctggaagt cgatgttgtc gtagttctgc aacagcaacg ccagattctc
     3361 ccacggcgag aaggtgtcgc gagtgagata gagaaacgta tagaacgctt catcgccgct
     3421 cacattcatt ccatagcgtt ttagcgccag ccagaacatg ccaacaatcc ccagcacgcc
     3481 cgccgccgcc agcatccaca acgaaatcca gccgcgaata atgccaataa acaggaagat
     3541 agcgaatgcg atgatgatat tggcgcgagt gccgccgaca atcatataag tcagcaagcc
     3601 aaaggcgacc gtgctgacga ggaaaaacag ccacgctttg ctgtcctggc gcagaaagta
     3661 gaccaccagc atcgccggga tgaaaaagta aaagaagcgt tttaacgcca cgccggagac
     3721 ttcactggaa aagatctgac tgtaggagtt aagccggaac agcaaaaagc cgttgtgcat
     3781 gaagaagatg ccgacgctta ccagcgcgat acccatcagg atcacccacg taagattggt
     3841 ttccacgcgg ttcatggtaa acagcggacg gcgcggtaca tcagcaacgc gtttgcgtag
     3901 gcgggttttg taggtgacgt agtaaaccgc gtagaagcag cccgcagaaa gcaacgcctg
     3961 caacaggatt tctggcggcg cgacaccaac atcaaagcga aacaccagca cgctggtcag
     4021 cgggaagcca aagaaaaagg tgagcagaaa cagcaatgaa aagaatacat tgaagttaaa
     4081 gcgcacacga cgaaactcaa accaggtcag cgtggcaata aacagcgtgc agagcagcca
     4141 gacaacaaac aggccactga attgcagcag actcatgcga cctccccggc ggcaatcgcc
     4201 aacgcccgct gccagccttg tagatagtta gggctaaaga aggcaatggt gtttttatcc
     4261 accgacgcca actggcgctg cgcttcacgc acaatatcct cgttgagatc gtcggtagta
     4321 aacagcaccg gtaaatgctg ttccgtcata tcctgccaga acggattttc ccggttaagc
     4381 acacaaggaa tgcccgcctg aatcagtaag cacagcgtac caatgccctg ctggcgggca
     4441 aaaataaagt aaccaagagc gcactgacga agtagcgtca gataggcgtc aaattccagt
     4501 ttttcactca gaacttgcag attttcttcg ctgaataact ccagccccgc ctgacgaact
     4561 tcctcgatgt acgcttcgtt atgaggcgga tatcccatcg gcaccaccac ttttaccgta
     4621 tcgccaaatt gctgatgaac ggcgcgcaag gcagcaatat gctcattgct gcggtcgccg
     4681 gagttcccca ccagaatggt cattttccct tcacgttgcc gatcgttcgc catcgtattg
     4741 agagaagcat ccatccgcgt cgggaagtac agcagttcgc cccgcacctt tgggtgcgtt
     4801 ttggcaaaaa agctcaaatc accacgggtg gcaaatacac agccgactcg cttttgcgcc
     4861 aggcgacgta gtgggtaaaa aagcttatat ctcaagccac tggaaagctc gtacaggtct
     4921 gccccccaga tatgccagta aaactggctg ggcttaatgc caccactcag cagagccagc
     4981 cacagtgtgg gattgaactg accgtggaag aaaaaacgct gctgacggtt agcttttgct
     5041 ttcgcgatga ccgcttccgc cagcgatttt ttcccaggga aaaattgcac agaaagcgcc
     5101 ggacagctat cacttaagcc gtcgtccttg ccaacaacca taaactcgcg cgcgtgctcg
     5161 ctcgtcgcgg ccagcgcgtc attgaaaaac cgcaaaacgg ttcggttatg gtgagggata
     5221 tccgatccca gtacgtgaat cagtacagtc atgcccgcct acgccagagt aaaaacacgc
     5281 cacaacaaag agaaaaatag acgatatagg ttgccatata tgcctgtgcc gcgcccagcg
     5341 cgccgtgcgt agggattagc cagtgggcaa ataccatcaa taaagtgaac tggctgactt
     5401 ccgccagaat ataaaaccgc agtgaagctt tggcgatcac cagataacca aagacataag
     5461 cgcccacttt taacacatca cccaccaact gccaggcaaa gagatcgcgc atagcggtaa
     5521 atttattcga caacagcagc cagatagcaa aatcacgcag caaccagacg gtaaaactcg
     5581 ccgccgccac tgccggtaag acgaatttca gcgatttaac cacttcccgg gtgatatcgc
     5641 gcttttccgt tagccgcgac aacgtgggca gcaaatatac gctgaacgat gccgtaataa
     5701 attgcaggta ggcatcggaa atactgctca ccccttgcca gatccccacc tcatcccagc
     5761 tatactgcgc cgccagcagt ttacgcatca tgatgtaagc aacaggcaag gtcaccgacg
     5821 taatcaacgt catgagcgta aatttgctca actgccctgc cagaccgtta tcccagctgg
     5881 gtttcagata gcttaacggg atagcaccac gcttaattaa catgacggcg gcaggaatca
     5941 ccaccagcgc gggaatcaac gccagaccca gcaacgcccc ttcatatccg cccaaacggt
     6001 aactgacgta gtacgcaaga acgccaatca agctgccaac aatcagagat aacgcattac
     6061 ctgcggcatc gcgaaagcct ttcatcaacg ccagtaacag gttgccccag gcgatcccca
     6121 tttgcaccag cgccactaaa cgcaccagcc cctgatagtc gtgattacca aacaatccca
     6181 agctgattgg cgcagctgcc agcacaaaaa ccagcgccat cagcgtagag aagccaagta
     6241 ccatcgctga tgaagtgccg accacgcggc gcagctgttg cggattatca tggtactggg
     6301 caacgtattt ggttacaccg ttaaagatgc cagccccggc aagcacgccg agcacggtaa
     6361 tcaactggcg gaaatttgcc gccagcccaa gccccgccgg accaaatgac accgccagca
     6421 acttaccgac cagtaacccg gcaccaatct tgaccagtgt actggccgcc gtccacaagg
     6481 acgcttttgc caacgacata tcaggagaag tagttcaaca aagtcgcaat taccgtacgc
     6541 tgattgacgg gcgacaggtt gtagaacagc ggcaggcgca gcaggcgctc gctctctttg
     6601 gtggtgtagc gatcttcacc gtggaactca ccaaagcgtt cccccgcagg gcaaccgtgc
     6661 agcggaatgt aatggaacac cgccatgatt tccgcttctt tcagaaagtt aatcaacgcg
     6721 ctccggtcac caatatcccg cagtttaatg taaaacatat gcgcgttctg caagcagcca
     6781 tcgggaatcg acggcagctc gatacgcccg gctttcgcca gaggtgctaa cgcatcgtag
     6841 tagttttgcc acagcgccag acgttgctgg ttgatacgat ccgctgcttc cagttgcgcc
     6901 cacaggtacg cagcttgcag atcggacatc aaatagctgg agccgatatc gcgccaggta
     6961 tatttatcga cctgaccacg gaagaactgg ctgcggtttg tgcctttttc acggatgatc
     7021 tcggctcgtt cgatcaacgc tttatcgtta atcagcgtcg cgccgccttc accgcccgcc
     7081 gtgtagtttt tggtttcatg gaagctaaag cagccaatat gaccaatggt tcccagtgca
     7141 cgccctttgt aagtggacat cacgccctga gcagcatctt ccaccacaaa cagattatgc
     7201 tttttcgcca acgccataat ggtgtccatt tcgcaggcca cgcccgcgta atgcaccggc
     7261 acgataacgc gcgttttgtc ggtgatcgcc gcttcaatca acgtttcgtc gatgttcatg
     7321 gtgtccgggc gaacatccac aaaaacgatt tttgcgccac gcagcacaaa ggcattggcg
     7381 gtggagacaa aggtgtagct cggcatgatc acttcatcgc caggctggat atcgagcagc
     7441 agcgccgcca tctccagcga agcggtgcag gacggcgtca gtaacacttt ggcgctgcca
     7501 aaacgttgct ccagccactg ctggcagcga cgggtaaaac cgccatcgcc acacagtttg
     7561 ccgctaccca ttgccgactg catatagtcg agttcggttc ccaccaccgg cggtgcgtta
     7621 aatggaatca tgtgatcacc tgtataacca gtacgcggtg ctttctacat tcgcaccact
     7681 ttgtatgtat cgtttaagcg cggcggtgtt gcccatttgg gtcgccaccc gcaaagttgt
     7741 tttaccgcga gcatacgccc agtttagcgc cgtttgcatc agctcagcac ctgcaccgcg
     7801 tccagccagc aggccaattc gcgcatctgt agcattgagt tcccgtaaag agacataacc
     7861 gcgaatatcg ccggacgccg cacgtaaaat cagacattga tgatcaaagg tgccgcgcac
     7921 ggcattttca atccactgtg cataaaagcg actgctggcg tcaggcgcat accacggcgc
     7981 acgaaaacgg ctttgcgcaa atgcggcgct ggctaactga cgtaatgcgg gaatatcggt
     8041 ctcttgtgcc actacagcac cgctatcact ggcattgttc acgggtagcg ccaaatcaac
     8101 ttcaccttct accagggaga atcccagctg ttgcagggca tccagttccc ccgtatttga
     8161 tgccgcaatt ttggcctgca cccgtgacca cggcgctaac gcgtctggcg tcaggaacgg
     8221 tgcttcagac gtaatgcgca cgatggcgct gttaacacca aagaaggcgt tttcccaggt
     8281 tagtggctca atactggcgc ggacgggcac gaagtaactc cagcagatat tggccgtagc
     8341 cagttttcgc taatgaactg gcagcacgct tcacaccctc gtcatcgagc cagccgttac
     8401 gccaggcgat ctcttccagg caggcaatct taaagccctg gcgtttttcc accgtctgta
     8461 caaaggtgct ggcttcaatc aggctgtcgt gagtgccagt gtccagccag gcaaatccgc
     8521 gcccgagcag ttcaacggtc aggttgcccg cctcgaggta catctggttg atggaggtaa
     8581 tctccagttc accacgctcc gacggcttca cctgctttgc gtactccacg actttactgt
     8641 cgtagaaata aagcccggtc accgcccagt ttgatttcgg ctgttttggc ttttcttcca
     8701 gcgagatagc gcggaaatta tcgtcaaact ccaccacgcc aaagcgttcc gggtccatca
     8761 cctgatagcc aaaaaccgtc gccacttccg tgcgcgccgc aacatgacgc agcttcggac
     8821 tgaagccctg accgaagaag atgttatcgc ccagcaccag acaagaaggt tcaccattaa
     8881 ggaaggtttc accgatgata aaggcctgcg ccagaccgtc cgggctgggc tgttcggcat
     8941 attccagctg gataccgaac tcactaccat cgcccagcag gcgctggaaa taacctttat
     9001 cttccggcgt agtgatgatg agaatttcgc ggataccggc cagcatcagc accgacagcg
     9061 gatagtaaat cattggctta tcgtaaatcg gcaacagttg cttcgatacg ccgcgcgtaa
     9121 tcggatgcaa tcgggtaccg gaaccgcccg ccaggataat acctttcatg tttgcctccg
     9181 ctgaaaatta gcctttcaga cctaaacgct cgccctgata gctgccgtcc tgcacctgct
     9241 tcctccagct ttcattagcc agataccact gcaccgtttt acgcattcca ctttcaaagg
     9301 tttcctgcgg cagccagcca agttcacggg caattttcga agcatcaatg gcatagcgca
     9361 gatcatgccc cggacggtca gcgacaaagg tgatcaagtc acgataatgc accacgcctt
     9421 gcggcttgtt cggagccagt tcttccagca gctcgcaaat ggtttccaca acatcgagat
     9481 tcttacgctc gttgtgacca ccaatattat aggtttcacc gactttcccg gtggtcgcca
     9541 cgcaatacag cgcgcgggcg tgatcttcaa catacagcca gtcacggatt tgctgcccgt
     9601 tgccatatac cggtagcggt ttacccgcca gcgcgttgag gatcatcagc gggatcagtt
     9661 tttccggaaa gtggtaagga ccgtagttat tcgagcagtt ggtgataagc gttggcagac
     9721 cataggttcg cagccaggcg cgcaccagat ggtcgctgct ggcttttgac gcggaataag
     9781 ggctgctcgg cgcatacggc gtggtttcgg tgaagaaatc atccgtcgag tgcaggtcac
     9841 catatacttc gtcagtggag atatgatgaa aacggaacgc tgatttttta tcttccgtca
     9901 gcgcattcca gtaagcccgc gccgcttcaa gcaatgtata agtcccgaca atgttggttt
     9961 caataaacgc tgccgggccg tcaatagaac ggtcaacatg gctttcggct gccagatgca
    10021 tgacacagtc tggctgatgc tcagtgaata cgcgcgccag ttctgcccga tcgcagatat
    10081 caactttctc aaaggcaaag cgctcgcttt gcgcgaccgg tgccagcgac atcaggtttc
    10141 cggcgtaggt cagcttatcg accactacca ccgcgtcgct cgtttcgttg atgatataac
    10201 gcaccagcgc cgagccaata aacccggcac cgcctgttat cagaatcttt ctcatcagcg
    10261 ccagactcct ttagcatcga cgacatactg ctgatggaca ttgtcgccat tgataacttt
    10321 gaactgacta tgatcgacca gcatcaccag cacgtctgcc gttgccagcg cctcgtcaag
    10381 ctgcgccaga gtacaaagcc cggtcagttt tttcggcaac tggtggatgt taggctcaac
    10441 aaccagagtt tcgccgctat gccactgggc gatcagttca gcgatttcca tcgccgggct
    10501 ttcgcgcagg tcatcaatat tcggtttaaa cgccagacca aagcaggcga ttttcagttc
    10561 actggcgcgt ttatcggtag ccgccaggca atcagccacc gccgctttca cctgatcgat
    10621 aacccagaac ggtttgtgat cgttcacttc gcgcgcggta cggataagcc gcgcctgctg
    10681 ggggttctgt gccacgataa accacggatc aacagcaatg cagtgaccgc ccacgccagg
    10741 gccaggctga agaatattaa cgcgagggtg acgattcgcc aggcgaatca gttcccagac
    10801 gttaatcccc tgatcggcac aaatcagcga caattcatta gcaaaagcga tattcacatc
    10861 gcggaagctg ttttcggtga gcttacacat ttccgccgtc cgcgagttag tgacgacaca
    10921 ctcaccttcg aggaaaattt tgtacagttc gctggcgcgg gccgaacaaa ccggcgtcat
    10981 accaccaatc acgcgatcgt ttttaatcag ctcgaccata acctggcccg gtaacacgcg
    11041 ttccgggcag taagcaatgt tgacgtccgc ctgctcgccc acctgctgtg ggaaagtgag
    11101 atccggacgc atctctgcta accattctgc catcttctcg gttgacccca ccggcgaggt
    11161 ggattcaagg atcaccagcg cgcctttttt cagcactggc gcaatggagc gagcagccga
    11221 ttcaacgtag gtcatatctg gctcatgatc gcctttaaac ggcgtgggta cagcaatcag
    11281 ccaggcatcc gcttcaactg gcgtcgtgct cgctcgtaaa aaaccgcctt ctacggcagt
    11341 ttttactacg ctcgccaaat caggctcgac gatatggatt tcgccacgat tgatggtatc
    11401 aaccgcgtgt tggttgatat cgacaccaat cacctgtttt tgccgtgagg caaacgctgc
    11461 tgctgtaggc agcccgatgt aacccagtcc gataacagaa atggtcgcaa aactcatagt
    11521 gatatccgat tattttttaa cgcttccaga atgcgagagc atgcctgacc atcaccatac
    11581 gggttatggg cgcggctcat agtttgatat tcgttttcgt cttttaaaag acgcgtcact
    11641 tcctcgacaa ttcgctgctt atccgtgcct accagacgca ccgtacccgc cgtcaccgct
    11701 tccggacgct cagtggtatc gcgcatcacc agcaccggtt tccccagcga aggcgcttct
    11761 tcctgaatgc cgcctgagtc ggtcaaaatc agccaggcgt ggttcatcag ccagacaaac
    11821 ggtaaatact cctgaggatc gatcagaatg acatttttca catgccccag aatgcgattg
    11881 accggctctc tgacgttcgg gttgagatgc accggataga caatctggat gtcctggtgc
    11941 gtggtggcga tgtctgccag cgcgtggcag atttcttcaa agccacgacc gaagctttca
    12001 cgcctgtgac cggtcaccag aatcatcttt ttatcggggt cgataaacgg gtaatttgcc
    12061 gcaagttctg aacgcagcgt gtcgctgctc atcacctggt cacgcaccca taacagtgca
    12121 tcaatgactg tattaccggt aatgaagatt cggctatctg caacgttttc acgcagcaag
    12181 ttttgccggg aagtttcggt tggagagaaa tgatacatcg ccagatgccc ggtcaatgta
    12241 cggttagcct cttccggcca cggcgaatag agatcgcccg tgcgtaaacc agcctcaacg
    12301 tgaccaacag gaatacgctg ataaaacgcc gccaggctgg ttgccagcgt cgtcgttgtg
    12361 tcgccgtgaa ccagcacgac gtctggtttg aactcggcaa gaataggttt tagcccttcc
    12421 agaatccgac aggttatctc tgtcaggccc tgtcctggct gcattatgtt gagatcgtag
    12481 tcaggtacaa tggaaaagag tttcagcacc tgatcgagca tctcccgatg ctgcgcagtg
    12541 acgcaaactt tagcctcaaa aaaaggatct tttgccaacg catgcaccaa cggagccatc
    12601 ttgatggctt ccgggcgcgt accaaataca gtcagtactt tcacatcgat tctcttcgaa
    12661 taagcggcga gcgcctttgc gctcaccgca gcagtgttgc tatttcgagc aacggcgggt
    12721 taatgcgaca ccagccccga tcagcccccc gacaatgccc cacataatca tcaggaaggc
    12781 acgacgtggg ctatcgcgtt ttaccggttc ttccggcgta cgcaaatagc gataggtctg
    12841 aaaacgcgga tccagggttg gaccaacatt cagggtgttt aacatggccc gattctgatc
    12901 atagtcgaga tcaaaggccg gaccgacggc ctgtaaattt tccagtcgag cctggagcat
    12961 tggacgccca agcaggaaca tttctgaatc aggtaattcc tcggcaggca catctgtcgc
    13021 actgcgcgaa atattatgct gctcagcaat tttcagcgcc tgctcaatgc tgttcatccg
    13081 gcggtcgtag atggctttcg ccacctcttc ctgacgcttc acctgtgctt tcatctggat
    13141 ggtacgcgcc gcccatgcgc ctttcagctc atcattcaga tggctggctg cacgctggct
    13201 ggcaaaagca acatactgac gtaacaggtt attagcgtca ggcgcggttt cggcaataag
    13261 cttcacgctg tcattgaccg cgcgggtaaa gtctccgggg ataaactgga tgttgttaat
    13321 catttcatcc agcaacgccg catcggcttt gctgttgccc accatccgct gcttgtaata
    13381 gtcggtttgc agccagaact ctctgcgggt atcccacgag gccagttgca taacaaactc
    13441 tttataggct tcgtccatga ccgatggttg gtcggcagaa gccatgtttg aacggacatc
    13501 caggttacgc aaaaattgct gctgcgagta atatcccccc agcatattca ccgttggacg
    13561 atcggtaatc gccgtcgagc tccactcctg acgagcaaaa aacgtatacg ccagcgcgat
    13621 taacgcaaac gccagcccca tgccaataat ccatagcttc ccagcccaca aggtacgaaa
    13681 caacccacga atatccagtt cattttcagc gtcttcggcc ggtttcccag gcattggttg
    13741 tgtcatcaca tcctcattta tttggttaaa ttggggctgc caccacgatt tctacgcagt
    13801 ctgcgtttta cgcgcttaat aaagcgagca actttccagg cacgcttaat gcaatatcca
    13861 tagaggagga atgctagcaa aaagagcacc aacatgaccc actccgggac aaaatgagaa
    13921 tattctgcca gcacgccaat ggaagcgagc agtgctgcgg caagggtaat cagcacaaac
    13981 gcctggcggg aagtaaaccc ggcacgcatg atcaaatggt gaatatgctg acggtcagga
    14041 gagaatgggc tcatgccttt acgcaggcga cggtacataa tcgccaccat atccattagc
    14101 ggaatggcga ttatccacaa agcggtaacc gggctgatgg gatgggtttt gccttgggtc
    14161 gtttcgagca ggatccagat cacggtaaaa ccaatcagcg tactgcccgc atcacccata
    14221 aagactttgt agcggcgacc caggatacca aggttaagca tgatatatgg caggatggcg
    14281 gcgatcatcg caaagcacca gattgcgagg ctggtttgcc cgtcgaacca caaaatcata
    14341 ccgattgctg caaacgagac gcaggacaac ccgcccagca agccatcaat gccatcaacc
    14401 atgttgaacg cattaatggc cgcccagacg gcaaatagcg tcaggaagta accaaacggt
    14461 ccgagcacca tctcccagga gccaaagata taacccaggc tactgagata aaggttgcca
    14521 aacaccatca taacaatgcc aacagcggcc tgtatggtgg cacggatttt tacgctgata
    14581 tcaaaacggt catccagcgc gccaatgaaa acaagcacac cggcacaagc gagatagaga
    14641 gatgcatgcg gaatatagta atcgacaatt ccgaacgtga agcaaatccc tgcgtaaacc
    14701 gaaatacccc caacgagtgg tatcaacccc tggtgacgtt tgcggaagtt tggtttatcc
    14761 actaaaccga ctttttttgc caccttacgg gcaaaaaaca gaaacagtgt cgtgaataaa
    14821 aaaatactga tgagatcagt actcactgtc agtaaattca caatgcatgc tctcagagaa
    14881 aattattagc agaagtataa ccacgaagac ctttattcag aagggaaagt ccgttccgaa
    14941 cccaccagtc ctgtgcaaaa attaaccatt tgatatgcaa attgcataac atgccagcaa
    15001 attccaggaa ttttcctaac atcgccagcg cggcattaag attacagata taaaagcaaa
    15061 acgccacgta aacacgtggc gtttttggca taagacaaat ttatgagcgt ttcatcattt
    15121 cgaagaaatc gtcattggtc ttggtcattg ccagtttatt aatgaggaat tccattgcat
    15181 cgatttcgcc catcgggtga atgattttgc gcaggatcca cattttctgc agttcttcct
    15241 gagtcgtgag cagctcttct ttacgggtac cggaacggtt gtagtcgata gccgggaaga
    15301 cgcgtttttc agcgatctta cgagagaggt gcagttccat gttgcctgta cctttaaact
    15361 cttcgtaaat aacttcgtcc attttagaac cggtatcgat aagcgccgtc gcgataatgg
    15421 tcaggctgcc gccctcttcc acgttacgcg ccgcaccgaa gaagcgtttc ggacgatgca
    15481 gggcgttggc atccacacca ccggtcaaca ctttacctga cgccggaaca acggtgttgt
    15541 aagcgcgcgc cagacgagtg atggagtcga gcagaatgat aacgtctttc ttgtgctcaa
    15601 ccaggcgttt cgccttctcg atcaccattt ccgcaacctg aacgtggcga gatgcgggtt
    15661 cgtcaaaggt agaagcaaca acttcacctt ttaccagtcg ctgcatctcg gttacttctt
    15721 ccggacgttc gtcgatcagc agaaccatca gcacacaatc cgggtggttg taagcaatgc
    15781 tctgagcaat gttctgcagc agcatggttt taccggcttt cggcggtgcc acaatcagac
    15841 cacgctgacc acgaccgata ggtgatgcca gatccagtac gcgagcggtt aaatcttcag
    15901 tagaaccgtt accacgttcc atacgcagac gagagtttgc gtgcagcggg gttaagttct
    15961 caaagaggat tttgttgcgg gcgttttcag gtttgtcgaa gttaacttcg ttaactttca
    16021 gcagcgcaaa atagcgttca ccttctttcg gcgggcgaat cttaccagag atggtatcac
    16081 cagtgcggag gttgaaacgg cggatttggc taggggaaac gtagatgtca tcaggaccgg
    16141 cgaggtagga gctgtctgcg gaacggagga aaccaaatcc atcctgcaat atctccagta
    16201 cgccatcacc aaagatatct tcgccactct ttgcgtgctg cttcaggatg gcaaaaataa
    16261 tgtcctgctt acgcatacga gccaggtttt ccagccccat attttcgccg agagtgatca
    16321 gctcagaaac cggcgtattc tttaattcgg taagattcat agtggtgtga gttcttaaac
    16381 ttggggtaaa tctcgaactt aatgttgtga atggtatggc aggatcatcc atgcctgttt
    16441 acggtcatcg actcatgtct tttcgctgcc tggtcacagg agagtacgca gaactgaaac
    16501 gacaagacgg attgagtgac gagccagaaa tctgttcact tcgcatttaa gataaaacgg
    16561 gaagcgttgg aaattacaag attcaaactt aataaggtat gtttaatacg aagtcaacac
    16621 taagttagca tgactcacgc cgggcgtcca gtttttagcg acggggcacc cgaacatgaa
    16681 attcccttac gccaggttag cgtcgaggaa ctctttcaac tgacctttag acagtgcgcc
    16741 cactttggtt gccgccactt caccgttttt gaacagcagc agagtcggga taccacggat
    16801 gccatatttc ggcgcagtgc cagggttttg atcgatgttc agttttgcaa cggtcagttt
    16861 gccctgatat tcgtcagcga tttcatccag aatcggggcg atcattttgc acggaccgca
    16921 ccactctgcc cagaaatcga cgaggatcgc cccgtccgct ttgagtacat ccgtgtcaaa
    16981 actgtcgtca gtcaggtgaa taattttatc gctcatatat aactccacag gaataagcct
    17041 ggcgtgttgg tttcgttgtt ggtgtaacat taaccaacta aaggttgact ttatttcacc
    17101 ggatacgctt tcgtaaagca atagtaagct gatattctac cacactatga gcaaaacaca
    17161 tttaacagaa cagaagtttt ccgacttcgc cctgcatccg aaggttgtag aagcccttga
    17221 aaaaaaaggg tttcataact gtacgcccat tcaggcactg gcccttccgc tgacgctggc
    17281 gggtcgtgac gtagccgggc aggcgcaaac cggtaccggg aaaacgatgg cgtttcttac
    17341 gtcaacgttt cattgtcttc tctctcatcc cgcgattgcc gatcgcaagg tgaatcagcc
    17401 gcgtgcctta attatggcac cgacgcgtga acttgccgtg cagatccatg ccgatgcgga
    17461 accgctggcg gaagctaccg gcctgaagct gggtctggct tacggtggtg atggttacga
    17521 caaacagctg aaagtgctgg aaagcggcgt tgacattctg attggcacca cggggcgttt
    17581 aattgactac gccaagcaga accacattaa cctcggtgcc attcaggtag tggtactgga
    17641 cgaagccgat cgcatgtacg atctgggctt tattaaagat atccgctggc tgttccgccg
    17701 tatgccgcct gcaaaccagc gcctcaacat gctgttctcc gccacgcttt cgtaccgggt
    17761 acgtgaactg gcgttcgagc agatgaacaa tgccgaatat attgaagtgg aaccggaaca
    17821 gaaaacgggc caccgtatta aagaagagct tttctaccct tctaacgaag aaaaaatgcg
    17881 tttgctgcaa acgctgatcg aagaagagtg gccagacaga gcgattattt tcgccaatac
    17941 caaacaccgt tgtgaagaga tctggggcca cctggcggca gatggtcatc gtgtcggttt
    18001 gttgacaggc gatgtcgcgc agaaaaaacg tctgcgtatt cttgatgaat ttacccgtgg
    18061 cgatctggat attctggttg ccaccgacgt tgccgcgcgt ggtttgcata ttccggcagt
    18121 gacgcacgtc tttaactacg atttacccga tgactgtgaa gattacgttc accgtattgg
    18181 tcgtacaggt cgcgcaggcg caaacggtca ctctattagc ctggcgtgtg aagagtatgc
    18241 attgaatttg cctgctattg agacctatat tggtcactca attccggtaa gcaaatacaa
    18301 tccggacgca ttgatgaccg atctgccaaa accgctgcgc ctcacgcgcc cgcgcacagg
    18361 caatggtccg cgtcgtactg gcgctccgcg taatcgtcgt cgttcaggtt aaataaaaaa
    18421 tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat cccgatgagc
    18481 ttactgtagt aagtgattca ggtgaaagaa tgcagccaac acagagacag attgaaggat
    18541 gaagagtatg ggttccacct catcgctgta tgcagccatt gatctcggtt cgaatagttt
    18601 tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc gaataaaacg
    18661 caaagtgcgt ctggctgctg gcctgaatag cgaaaatgcc ctctccaatg aagcaatgga
    18721 gcgcggttgg caatgtttgc gcctgtttgc tgaacgtctg caagatattc ctccctcgca
    18781 aattcgcgtt gtcgctacgg cgacgttacg ccttgccgtc aatgcgggtg attttattgc
    18841 caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag aggaagcacg
    18901 tctgatttat cagggtgttg ctcacaccac tggcggtgcc gatcagcgcc tggtagtgga
    18961 tatcggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca cctcgttgtt
    19021 cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc gtaatctggg
    19081 gcaggaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc cggttgccga
    19141 tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg tgcaggcgtt
    19201 acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa agttgcagca
    19261 attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg acgggctgac
    19321 gctggaacgt gcgttagtgt tcccgagtgg tctggcgatc ctgatcgcca tttttaccga
    19381 actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc tggtctacgg
    19441 catgttgcat ctggccgtcg agcaggatat tcgcagccgt acgctgcgta atattcaacg
    19501 ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg ctaacttctt
    19561 cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc tcatcagcgc
    19621 ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagcgccgc aacacgctgc
    19681 ttatctggtg cgtaatctgg atcttcccgg ttttaccccc gcacagaaaa aattgctggc
    19741 gacgctactg ctcaaccaga ctaatccggt cgatctctca tcgctgcatc agcaaaatgc
    19801 cgtaccgccg cgcgtcgcag aacaactctg ccgtttacta cgcctggcca tcatttttgc
    19861 cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc atgaactgtt
    19921 gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag agattattgc
    19981 tcaggaaagc cagtggcaga gctatgtcca ctggccgctg gaagtgcatt aatgagtaag
    20041 tgccggatgc gatgctgacg catcttttcc ggccttgatt atttccctcg ttttgccgcc
    20101 atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc ttcggcgctg
    20161 accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag caaaaagcgg
    20221 ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag cgtaaaggtc
    20281 aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc atcgatatta
    20341 tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac catgtagaca
    20401 taaggaaact ccagtccttt tgacgcgtgg agagtcatca gttgcacctg atccagctct
    20461 tcttcactct caccacgctc catcatgtcg cgcaaagtaa agcgcgtcac cacctgggtg
    20521 agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca gctaaacagt
    20581 tggttgacgt tcttcatgcg catttcggcg gctttcgggc tgggcgatgt ttcgtacagc
    20641 caggattcat aatccatgcc atggatcaga tcacgcaccg cagcaatcgg ctcccgctcc
    20701 gccaggcgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc ataaccacgt
    20761 ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct tttattgcgc
    20821 gtcatcgccc actcacccag ctttttcagc gtagccgggc caatctctcg cttcggcgtg
    20881 ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg cagataagcc
    20941 agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccggatat tttgtacggg
    21001 atgcggtttt gcatcaggaa tttttcaaat acccgcgact gatggttgcc gcgataaaga
    21061 atggcgtaat ctttgtactg cgttttattg acgaagtgat gggcgatcag ctcgccagta
    21121 acgcgctcgg cttcatgttc ctcgttattc gcgcttaaaa cttttagctc tgtgccgtaa
    21181 cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat caggatgttc
    21241 gccgctttca gaatacgccc ggaagagcga tagttctgct caagcttaat caccttcagc
    21301 gccggaaaat cctgactcag cagcaccagg ttttgcggac gtgcaccgcg ccaggagtag
    21361 atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac cagcagtttc
    21421 accagttcat actggctggt gttggtgtcc tgatactcat ccaccagcag gtagcgaatt
    21481 ttgttctgcc agcgttcgcg tacttcttca ttgcgttgca gcagcaacgt cggcaataaa
    21541 atcaggtcat cgaagtcgag aacgttacag gctttcaggt gtgcatcata cagcccataa
    21601 caatgggcaa aaatacgatc ccgctcgccc tttgcctctg ccgccgcctg cgccggagtt
    21661 ttgagatcgt tcttccagtt agagatggtc gaaatcagtt gttgcaggag aactttgtca
    21721 tcttcaatca gcccctcggt caactcttta agcaaagcaa gctgatcggt atcgtcaaat
    21781 aacgagaagt tcgctttcat cccaagcgcc gcatactcgc gtttgatgat atccagcccc
    21841 aacgtatgga aagtggagat catcagccca cgcgcctctt tacgccccag cgtctgccct
    21901 acacgctctt tcatctcgcg cgctgcttta ttagtaaagg tcaccgccgc aatgtgccgc
    21961 gcctgataac cgcaaccgcg gatcagatgg gcgattttat tggtgataac acgagtttta
    22021 ccggaacccg cgcccgccag caccaggcag gggccggtaa cgaattcgac agcttgttgt
    22081 tggccggggt ttagacgcat aggtgtattg ctcaatcttc gaacgggggg aggattgtag
    22141 cagaatcgca tttgaatgtg accgcgtgta agccgctaac cggttggctg cggatgttta
    22201 aatcagagag agaagaccga aatcggagtt catcgtgaat ccattagact tagaaaatat
    22261 cgggtcgtaa cgttgtgtta ttactttttc agagtttact aaggagaggt ttactcgtgg
    22321 cgattaattt cagccctaag gttggtgaaa tactggaatg caactttggt aactatcctg
    22381 ttagccaaaa tggaccgttc agcacgacgt attacgatgg ccgcattcct cctgaaatga
    22441 taaaaaacag gttagttgtc gtgctcaacg gaaaaattaa tggcaacgca ttcatcgtcg
    22501 ttccattatc aaccactcgc gaccatgaca agttaaaacg tggaatgcat gttgaaatag
    22561 ccagtaatgt tatcaatgac ctgcaattct ttgatcagca gattcgttgg gccaaaaccg
    22621 acctggtaca acaagtcagt cgaaacagat taaacagagc aagaacttac agagggtatc
    22681 ttaatcaatg tttgccacat gagttggttg cagacataca gagggcggtc atcaagtcaa
    22741 tcaatgccat atcgctcata aattgacaat aaaatcacca tccccgataa tgactttgta
    22801 gcccatagag gccctaagtt ttctcccctt gagggacctg cgaagcccgg attttccggg
    22861 cttttgccat atatgtacca gcccctccct gactgacaat tacgttagca tcggcggatc
    22921 atgctaaaat cgccgcctga caattattca ctcaaggcac gatcatggca aaaacagcag
    22981 cagcactgca tatccttgta aaagaagaga aactggctct ggatcttctc gagcagatta
    23041 agaacggggc cgattttggc aagctggcga agaaacactc catttgccca tcaggcaaac
    23101 gcggcggtga tttaggtgaa ttccgccagg gtcagatggt tccggcgttc gataaagttg
    23161 tgttctcttg tccggtactg gagccaaccg gcccgctgca cacccagttc ggttaccaca
    23221 tcattaaggt gctgtaccgt aactaatagc aaggccttct ccgggagaag gccttgaact
    23281 ctgaatcgct attaacccgc aacagcaata cgtttcatat cagtcatgta gccgcgcagt
    23341 ttcttaccta cctgctcaat cgcatggctg cgaatcgctt cgttcaaatc acgcagttgc
    23401 gcgttatcta ccgcaccttc cggaatagct ttacccaaat cgcccggttg cagctctgcc
    23461 ataaacggtt tcagcaacgg cacacaagcg taagagaaca gatagttacc gtactcagcg
    23521 gtatcagaga taaccacgtt catttcgtac agacgcttac gggcgatggt gttggcaatc
    23581 agcggcagtt cgtgcagtga ttcatagtaa gcagattctt cgatgatgcc ggaatcaacc
    23641 atggtttcaa acgccagctc aacgcccgct ttcaccatcg cgatcatcag tacgccttta
    23701 tcgaagtact cctgctcgcc gattttgcct tcatactgcg gcgcggtttc aaacgcggtt
    23761 ttgccggtct cttcacgcca ggtcagcagt ttcttatcat cgttggccca gtccgccatc
    23821 atgccggagg agaattcgcc ggagatgatg tcgtccatat gtttctggaa cagcggtgcc
    23881 atgatctctt tcagctgttc tgacaacgcg taagcgcgca gtttcgccgg gttagagaga
    23941 cggtccatca tcagggtgat gccgccctgt ttcagcgctt cggtgatggt ttcccagccg
    24001 aactgaatca gtttttctgc gtatgccgga tcggtgcctt cttccaccag cttgtcgaag
    24061 cacagcagag agccggcctg caacataccg cacagaatgg tttgctcgcc catcaggtca
    24121 gatttcactt ccgcaacgaa agaggattcc agcacgcccg cacgatgacc accggttgcc
    24181 gccgcccatg ctttagcgat cgccatgcct tcgcctttcg gatcgttttc cgggtgaacg
    24241 gcaatcagcg tcggtacgcc gaatccacgt ttgtactctt cacgtacttc ggtgcctggg
    24301 catttcggcg caaccattac tacggtgatg tctttacgga tctgctcgcc cacttcgacg
    24361 atgttgaaac catgcgagta acccagcgcc gcgccgtctt tcatcagtgg ctgtacggtg
    24421 tgcactacat cagagtgctg cttgtccggc gtcaggttaa ccaccagatc cgcctgtggg
    24481 atcagttctt cgtaagtacc cactttaaaa ccattttcgg tcgctttacg ccaggacgcg
    24541 cgcttctcgg caatcgcttc tttacgcaga gcgtaggaga tatcgagacc agaatcacgc
    24601 atgttcaggc cctggttcag accctgtgcg ccacagccga cgatgaccac ttttttaccc
    24661 tgaaggtagc tcgcgccatc ggcgaattcg tcgcggccca taaagcgaca tttgcccaac
    24721 tgtgccagtt gctggcgcag gttcagtgta ttgaagtagt tagccatggt gattcctcgt
    24781 gatgttgtgc ttcttattgt tcggttcgcg tttgcaagat tgaattcact atatgacagg
    24841 aaatttattg cggaaattga tatattcaca acatcatatt gcaatttttg caacgtcaac
    24901 atcgagggct gtccctgtgg atttacgcga tctgaaaacc ttcctgcatc tggcggaaag
    24961 ccgccatttt ggccgcagcg cgcgggcgat gcacgttagc ccatccacgc tctcacggca
    25021 gattcagcgc ctggaagaag atctcggtca gccgctgttt gtgcgcgata accgcacggt
    25081 gacgctgact gaagcgggcg aagaactgcg cgttttcgcc cagcaaacgc tgttgcagta
    25141 tcagcagctg cgccacacca tcgatcagca agggccgtcg ctctctggcg aattacatat
    25201 cttctgctcg gtgaccgctg cctacagcca tctgccgccg attctggatc gcttccgcgc
    25261 ggaacacccg tcggtggaga ttaaactcac tactggtgat gcggcagatg caatggaaaa
    25321 agtggtcacg ggagaagcgg atctggcgat tgccgggaaa ccggagacgc tacccggcgc
    25381 tgtagcgttt tcgatgctgg agaatctggc ggtagtgctg attgcgcccg cgctgccctg
    25441 cccggtgcgt aatcaggtct cggtagagaa gccggactgg tcaacggtac cgtttatcat
    25501 ggccgatcag gggccagtac gccgccgcat tgaactgtgg tttcgacgca ataaaatcag
    25561 caacccaatg atttacgcca cggttggcgg gcatgaagcg atggtatcga tggtggcgct
    25621 cggctgtggt gtggcgctgt tgccggaagt ggtgctggaa aacagcccgg agcctgtacg
    25681 caaccgggta atgattttag atcgcagcga tgagaaaacg ccgtttgaac tgggcgtgtg
    25741 tgcacaaaaa aagcggctgc atgagccgct gattgaggcg ttctggagga ttttgccgaa
    25801 ccacaaatga cgttgtcgcg cgagtaggcc tgataagcga agcgctatca ggcatttttc
    25861 cctaacccgc caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg caatcgtagc
    25921 ccagctcatt cagccgggtt tcgaaatccg gttcatggtc gccaagttcg aacgccgcca
    25981 gtacgcgccc gtagtcggtg ccatggctgc gatagtggaa caaagaaatg ttccagtgcg
    26041 tacccagcgt gttgaggaag cgcagcagcg cgcccggtga ttccgggaat tcgaagctgt
    26101 agaggcgttc ctgcaacgga tgtgacggac gcccgccgac catatagcgc acatgcaact
    26161 tcgccatttc gtcgtcggag agatccacca cgctgtagcc gccgtcgttg agcatctgca
    26221 aaatttcttt gcgctcttcg aggccgcggc tcaggcgcac gccgacaaag atgcaggcgt
    26281 ttttggcatc ggcaaaacgg tagttgaact cggtgaccga tcgcccgcca agcagttggc
    26341 agaatttgag gaagctgcct ttttcttccg gaatggtcac cgccaacaac gcttcacgct
    26401 gttcgcccag ttcgcagcgt tccgagacgt agcgcaggcc gtggaagttc acgttggcac
    26461 cggaaagaat atgcgccagc cgttcgccgc gaatgttgtg ctgggcgata tattttttca
    26521 ttcccgccag cgccagcgcg ccagagggtt ccgccaccgc gcgcacatct tcgaataaat
    26581 ccttcatcgc cgcacagatc gcatcactat cgacggtgat gatatcgtcg agatactcct
    26641 ggcacaaacg gaaggtttcg tcgccgatgc gttttaccgc aacgccttca gcaaacagcc
    26701 ccacgcgcgg cagatcaacc ggatgacccg catccagcgc tgctttcagg caggcggagt
    26761 cttccgcttc tacggcgatt actttgattt gcggcatcag ttgtttgatc agcaccgcca
    26821 cgccagcagc cagaccgccg ccgccgactg gcacaaatac gcggtcgaga tgggcgtcct
    26881 gctggagcag ttccagcgcc agcgtgcctt gcccggcaat caccatcgga tggtcgaacg
    26941 gcggcaccca ggtgaacccc tgctgctgtg acagttcgat cgctttggct ttcgcttcat
    27001 caaagttcgc gccgtggagc agcacttcgc cgccgaagcc gcgcaccgcg tcgactttga
    27061 tgtcggcggt ggcggttggc ataacgatca gggccttcac gcctaaccgc gcagaagaaa
    27121 acgcgacgcc ctgcgcgtgg ttacccgcag aagcagtgat cacgccgtgc gctttttgtt
    27181 cttccgtcag gcccgccatc atggcgtatg cgccgcgcag cttaaagctg tgcactggct
    27241 ggcgatcttc gcgcttcacc agaatcacgt tatcaagacg agacgagagc ttttccattt
    27301 tttgtagcgg cgtgacctgc gccgcttcgt agaccggcgc gcgcagcacc gctcttaaat
    27361 attcagcacc ttccggggca ccggacaggg gttgcgagtc agccattatt aaccccccag
    27421 tttcgattta tcgcgcaccg cgcctttgtc ggcgctggtt gccaggctgg cataagcacg
    27481 cagggcaaag gagatctgac gttcacgatt tttcggcgtc caggctttgt caccccgagc
    27541 ttcctgcgct tcacgacgcg ccgccagttc ggcatcgctt acctgtaact gaatgccacg
    27601 gttcggaatg tcgatggcga tcaggtcgcc gtcttcaatc aggccaatgc tgccgccgct
    27661 tgccgcttcc ggtgagacgt ggccgataga aaggccagag gtgccgccag agaaacgacc
    27721 gtcggtgatc agcgcacagg ctttaccgag ccccattgat ttcaggaagc tggttgggta
    27781 gagcatttcc tgcatccccg gaccaccttt tgggccttca tagcgaatta ctaccacatc
    27841 tccggcgaca actttaccgc cgagaatcgc ttctaccgcg tcgtcctggc tttcgtacac
    27901 tttcgccggg ccggtgaatt tgaggatgct gtcatcgacg cccgcagttt taacgatgca
    27961 gccgttttcc gcgaaattac cgtagagcac cgccaggccg ccgtctttgc tataggcgtg
    28021 ttccagcgag cggatacagc cattggcgcg atcgtcgtcc agcgtatccc aacggcaatc
    28081 ttgcgagaat gcctgggtgg tacggatacc cgcagggccg gcgcggaaca tattttttac
    28141 cgcgtcatcc tgggtcagca taacgtcgta ttgttccagc gtttgcggca acgtcaggcc
    28201 aagtacgttt ttcacatcac ggttcagtaa ccccgcacga tccagctcgc cgagaatacc
    28261 gataacacca ccagcacggt gaacatcttc catatggtat ttttgggtgc tcggcgcgac
    28321 tttgcataac tgcggaacct tgcgggaaag cttatcgata tcactcatgg tgaagtcgat
    28381 ttccgcttcc tgcgccgccg ccagcaggtg aagtacggtg ttagtcgatc cacccatcgc
    28441 gatatccagc gtcatggcat tttcaaacgc cgctttgcta gcaatattgc ggggcagtgc
    28501 actttcgtcg ttttgctcgt agtaacgttt ggtcaattca acaatgcgtt taccagcatt
    28561 gaggaacagc tgcttacggt cagagtgggt tgccagcagc gagccgttgc ccggctgcga
    28621 cagacccagc gcttcggtca ggcagttcat tgagttagcg gtaaacatcc cggagcagga
    28681 accgcaggtt gggcatgcgg aacgttcaac ctgatcgctc tgggagtcag atactttcgg
    28741 gtctgcgccc tggatcatcg catcaaccag atcgagcttg atgatctgat cggacagttt
    28801 ggttttcccg gcctccatcg ggccgccgga aacaaagatc accggaatat tcagacgcag
    28861 ggaagccatc agcatccccg gggtgatttt gtcacagtta gagatgcaga ccatggcgtc
    28921 ggcgcagtgg gcgttgacca tatactcaac ggaatcagcg atcagttcgc gagatggcag
    28981 tgaataaagc atccccccgt ggcccatggc aatcccatca tccaccgcaa tggtgttgaa
    29041 ctctttggca acgccgccag ccgcttcaat ttgttcggcg accagtttac cgagatcgcg
    29101 cagatggacg tgacccggta caaattgggt gaacgagttc acaaccgcga taatcggctt
    29161 accgaaatcg gcgtcggtca ttccggtggc gcgccacagc gcacgagcac ccgccatatt
    29221 acgaccatga gtggtggtgg cggaacggta cttaggcata ctttatttac tcccagtgtc
    29281 tgtctcgtaa atgggacggt gcgtgccgtc ccattttttg tatttattga ttaacttgat
    29341 ctaaccagcc ccatttatct tcggtttcgc cggtaaagag gccgaagaag gcttgctgaa
    29401 tgcgtttggt gaccgggcca caacggcctt cgccaacctg aataccgtct acactgcgca
    29461 ctggcgtgat ttctgccgcc gtaccggaca taaagacttc atccgccagg tacaaggatt
    29521 cgcgcgacag cacctgctca cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg
    29581 catcacgggt aatacccggc agcgcggagg aggtgaacgg tggggtgaac agcacaccat
    29641 ctttcacttc aaacaggttt tcgcctgcgc cttcagagat ataaccgttc acatccagcg
    29701 cgataccttc ctgataaccg tggcggcgcg cttcgctacc caccagcagg gaagagagat
    29761 agttaccacc ggcttttgcc gcagttggga tggtgtttgg tgctgcgcgg ttccaggagg
    29821 aaaccatcgc atcgatcccc tgctccagcg cttctgcgcc cagatacgct ccccacggga
    29881 aagcggcgat aatcacatcg gttgagtatc ccgctggcgg gtttacgccc atgccaacat
    29941 caccaacgaa gatcagcgga cggatatagg cgctggtgag attgtttttg cggatcacgt
    30001 cacggcaagc ttccatcagc tcatcaatgc tctgcgaaac cgggaagcga tagattttgg
    30061 cggagtcatg cagacgctgc atatgctcac gatggcggaa tacaaccggt cctttgtgcg
    30121 agtcgtagca acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg tgcgacatca
    30181 catgcacctt cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg taatcagctt
    30241 tcttcgtggt catttttata ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt
    30301 gtgctctggc agatggcaac gtgtgcgacg tccaccagtt tatttaactg actaaacagt
    30361 aagtcgaccg accgtgggct ggcaacggtc aattcgatat ttatattttg tgcatcgctg
    30421 gcggcggcca tattcattga gcagacgtgg aaaccacgat gacgcaccac gcgtaaaaca
    30481 cgttctaagg tttccggatt gaagcgagcc gatacattga cctgatgttg catcatgata
    30541 atttctccaa catttctgaa ttactggcac caggcggcac cagcggccag acgttctcaa
    30601 gttcgtcgat tgagacatga agcaggtatg gcccatcact gttcagcatg gtgtcgagtg
    30661 ccgcttcaac ctggtcttta cgggtgatgt gttggccagg gatgccgaag gcgctggcta
    30721 acttgaggaa atcggggtta tcggtaaggg tggtttcgct gtatcgttcc tggaaaaaca
    30781 gttgctgcca ttgtcgaacc atccctaacc gttggttatc gagtaagacg attttcaacg
    30841 gtaactgctt gcgttttacg gtgcccagct cttgtacatt catcatgaaa gagccgtcac
    30901 cggagataca gacgacggta tcgtttggtc gcgcgacttg cgcgccaacc gccgccggta
    30961 aaccaaaacc catggtgcct aagccgctgg aggtaatgaa attttccggg cgagtgtgtg
    31021 cgatgtgctg cgcggcccac atctgatgct gccccacatc tgtggtcaca acgcaatccg
    31081 caggtttacg atccgacagt tgttttaaca acaacggcgc gtagatagcg tcaccgggat
    31141 ggtcgtaacg ccaggcatgt tcatcacgca gctgcgcgca gtattgctgc cagtcattga
    31201 tatttaatgg ctgctgtaat gctggtaaca gcgcatttaa atcaccctgt aatgccacat
    31261 gtgcctgacg cagcttgttc atttctgccg ggtcgatatc catatggata acactggcgt
    31321 gtggtgcgaa ggtgttcaat ttgccggtca cccggtcatc aaaacgtgca cccacggcga
    31381 tcagcaagtc gcactcctgc accgcgaagt tcgccgcttt ggtgccatgc attcccagca
    31441 tgcccagata gtacggataa tctgcttcaa ctgcgcccag ccctttcagc gtgcaggtgg
    31501 caggcatttt tgtggtagcg agaaattctc gtaaagcagg aactgcctgc gccataccca
    31561 cgccaccacc aacgtacagc atcggttttt gcgcttttgc cagcatctgg cgcgcttgct
    31621 caacttcggc atgtgggaaa gtcacttcgt tttcaacggt ggtgaaccac ggttccaggt
    31681 cgccgctggc taactggata tcttttggga tatcgaccag aaccggacca ggacgacctg
    31741 agctggcaac gtcgaatgct tcagccataa tgcgcggcaa ctcttccagc gactgcacca
    31801 gaaagctgtg cttggtacag gctaacgaca atcccaggac atccacttcc tgaaatgcgt
    31861 cagtaccgat aaacggtgcg gacacttgac cggtgatggc aacaacaggg atagaatcta
    31921 acagtgcgtc cgcaagcccg gttatcaggt tggttgcgcc cggaccagac gtggcgatac
    31981 atacgccagt tttgccggta gcacgggcat aaccgatagc cgccattgcc gcaccctgct
    32041 catgtcggca cagcaagtgc tccacgccgc cgtcatacaa tgcatcgtaa accggcataa
    32101 ttgcgccacc cggataaccg aaaacggtgt tcacaccctg tgcccgcaac gcatgtacca
    32161 cccactgtgc gccattcata gttagttccc cgtcctgaat cttgggaaac agaattttgt
    32221 gctgttattc attgtctgct cctcggttat gtttttaagg tcaaaaaaaa cccccggacc
    32281 tttcggtgcg ggggtcttag ttcgttaagg cttgatctct aagcctttcc tcgtccaagt
    32341 gcagccccgc acggtgggat aataatcacc accacgctaa tcacgaccag gctaatcact
    32401 cgtagaaggg ctgtcatttt gtcttttctt gcatcttgtt cgaaggaatg cctaaagagt
    32461 taccatagat tttgcgaata gcacaagata ttttttggcc gttttttatg atggctttaa
    32521 taatttcaat agaaattgtt gttttttaaa aagaaaatag tgagtgaaaa aaattcaatt
    32581 tcagtacgaa tgttgttccc tccctcgtag ttcatccttt gtccgctgca tttttgcgag
    32641 catcattcca ccgccagaac aactcaactt cacttgcaca aataaacagg aatcagccac
    32701 taaaattcac gaattagcgg gctacagcag cgatatctta tgcacataat ggtgactcaa
    32761 ggagggctta tgtcactgtc aattgttcat acccgcgcag ccctgggagt aaatgcgccc
    32821 ccaatcactg ttgaggtaca tatcagtaaa ggtttgcccg gcttaacgat ggtgggctta
    32881 ccagaaacaa cggtaaaaga agctcgcgat cgcgtgcgca gcgccattat caatagcgga
    32941 tatgaatatc cggcgaaaaa aatcaccatt aaccttgctc cagccgatct accaaaagaa
    33001 gggggacgat atgatttacc tatcgccatt gcgttgctgg cggcctcaga acagcttaca
    33061 gccaataagt tagatgaata tgaattagtc ggagaactgg cgcttacagg cgctctgcgt
    33121 ggcgttcccg gtgcaatctc cagtgcaact gaagctatta agtcgggcag aaaaattatc
    33181 gtcgcgaaag ataacgaaga tgaagtgggg ctaattaacg gtgaaggatg cctggtagcc
    33241 gatcatctgc aagctgtctg tgcgtttctg gaaggtaagc acgctctcga acgcccgaaa
    33301 ccaactgatg cagtatcccg ggcgctacaa catgatctca gtgatgttgt cggtcaggag
    33361 caaggaaagc gaggactgga aattaccgcc gctggcgggc acaacctttt actgattggg
    33421 ccgccgggaa caggtaaaac aatgctcgcc agccgtatta atggtctttt gccagattta
    33481 agcaatgaag aggcactgga gagcgctgcg atattaagtc tggtaaatgc tgaatcagta
    33541 caaaaacaat ggcggcagcg cccgttccgc tcacctcatc acagtgcgtc gttaacggcg
    33601 atggtgggcg gtggtgcaat tccagggcca ggtgaaattt cgttggcgca taacggcgtg
    33661 ctttttcttg atgagctacc tgaatttgaa cggcgtacac tggatgcttt gcgagagccg
    33721 attgaatccg ggcagatcca tctttcacgc acacgagcaa aaataaccta tccagcccgt
    33781 ttccagcttg ttgcggcgat gaatcccagc cctaccggac attatcaggg aaaccataac
    33841 cgctgcacgc cagaacagac attacgttat ctcaaccggc tctcggggcc ctttctcgac
    33901 cgcttcgatc tctcactgga gatcccatta ccaccccccg gcattttgag taaaacggta
    33961 gtgccgggag aaagcagcac caccgttaaa caacgtgtaa tggctgccag agagcgccaa
    34021 tttaagcggc agaataagtt gaacgcctgg ctggatagtc cggaaatacg caaattctgc
    34081 aagcttgaga gcgaagatgc gcagtggctg gaagaaacgc tgatccatct ggggttatcg
    34141 attcgtgcct ggcagcggtt attgaaagtt gcacgaacca ttgctgatat tgatcagtct
    34201 gacattatca cacgtcagca tttgcaggag gcagttagct atcgtgcgat tgaccgtttg
    34261 ctcatccatc tgcaaaaact actgacataa aaaaagggca tttcgccctt tttattaatc
    34321 gtcagaatcg gtgtagtctt cagcaccttc aacctgcggt ttaccgccgg aaagggtgtg
    34381 aaaacgtttt ggacgcttga tacgcgtcat atacttggac cacacgcgtt ctgcttctgt
    34441 cactggctca cgttcgccac ggcatactgc tacgaagagt ttctcttcct cggtaaccgg
    34501 ctcgcgtttg ccaagatcca actcattgaa ggcataacca tgacgctcaa gcagttgtgc
    34561 ctctttgatg gtgaaatcac catggcgaga gaatccacgt ggataatgtt tattgtcgaa
    34621 atatcgatta gtcgtcgtaa agctttccgc catcctgcac gctcctaatt ctttgaccga
    34681 gctagttatg gcgcggagta ttagttacgc ttgacagagt gtaaaacaaa acatttaaat
    34741 cataacgaca aataattttg cggagagcac tgtggatacg gaattgttaa aaactttcct
    34801 ggaagttagc cgaacgcgtc actttggtcg agcggctgaa tcgctctatc tgacccagtc
    34861 agcagtgagc tttcgaatca gacaactgga aaatcaactg ggtgtgaacc ttttcacccg
    34921 ccacagaaac aatatccgtt taaccgctgc cggtgaaaaa ctactgcctt atgcagaaac
    34981 gctcatgagc acctggcagg ccgcccgtaa ggaggtggcg catacctcac gacataacga
    35041 gttttctatc ggtgccagcg cctcattgtg ggaatgtatg cttaatcagt ggctgggacg
    35101 tttgtatcaa aatcaggatg cccatacagg cttacagttc gaagcgcgaa ttgcccaacg
    35161 gcagtctctg gtaaaacagc tgcatgaacg ccagcttgat cttcttatta ccactgaagc
    35221 gcccaaaatg gacgaattta gtagtcagtt gctggggtat ttcactttag cgctttatac
    35281 cagtgcccct tctaaactaa agggagatct taactatctg cgactggagt gggggccaga
    35341 ttttcaacag catgaggcag gtttgatcgg tgctgacgaa gtgcccattc tgacaaccag
    35401 ttctgctgaa ctggcacagc aacagattgc gatgcttaat ggttgcacct ggctacccgt
    35461 cagctgggcg cgtaaaaaag gcggcctgca taccgttgtc gatagcacaa cactttcacg
    35521 gccgctttat gccatatggc tgcaaaatag cgataaaaat acgttgattc gcgatctatt
    35581 gaaaattaac gtgctggatg aagtgtatta atctgaatgg ctggcaagga tgccggtaga
    35641 aggatttact tcagagaggg ttatttcaga taaaaaaaat ccttagcttt cgctaaggat
    35701 gatttctggc aggggcggag agactcgaac tcccaacacc cggttttgga gaccggtgct
    35761 ctaccaattg aactacgccc ctaattaggg tggcggaacg gacgggactc gaacccgcga
    35821 ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccacc gaattctttt
    35881 acaaccaccg gttttatgac cggctt
//
LOCUS       DAZKIF010000035        35562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZKIF010000035 DAZKIF010000000
VERSION     DAZKIF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.035"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(66..548)
                     /locus_tag="ACHOTW_001745"
     CDS             complement(66..548)
                     /locus_tag="ACHOTW_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843868.1"
                     /translation="MVELVCFNDDDCAQVRELLKQSNGNITDDKIDEITACLSNPQGI
                     TCFYYSPLQGYQSFLKNTSQDVSELLSKSLNGTISSTSGLKKSGNAFFGTGKYLKEID
                     FTINGFSQKMYLMAVNNVGNEVKLQCSCYFTTGSVDEEKSKSDKIDFTFSGAATTADL
                     "
     gene            complement(696..1349)
                     /locus_tag="ACHOTW_001746"
     CDS             complement(696..1349)
                     /locus_tag="ACHOTW_001746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843869.1"
                     /translation="MASPINSGIMMANLCPSTFLDKNRNVAAELDIKNNEKKYSPGSN
                     FAKWMLQEIKRLILNIMSGSRSINTDILDYFHPMPGTENNGNRTWVAATGEDEYIEIK
                     QTGDKSFNITLVGRDKPSRKEIPYSGVAVATIIKSLSEKTSALETHSADTVLRKKLVN
                     SIVMKNTDFNYEIPAGILSNIYDLLKLRIKKDEGYVPVQESFKRTDVFFDSMIMDAH"
     gene            complement(1612..1902)
                     /gene="ilvN"
                     /locus_tag="ACHOTW_001747"
     CDS             complement(1612..1902)
                     /gene="ilvN"
                     /locus_tag="ACHOTW_001747"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9843870.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(1906..3594)
                     /gene="ilvB"
                     /locus_tag="ACHOTW_001748"
     CDS             complement(1906..3594)
                     /gene="ilvB"
                     /locus_tag="ACHOTW_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP9843871.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(3700..3798)
                     /gene="ivbL"
                     /locus_tag="ACHOTW_001749"
     CDS             complement(3700..3798)
                     /gene="ivbL"
                     /locus_tag="ACHOTW_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP9843872.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            4363..4452
                     /gene="tisB"
                     /locus_tag="ACHOTW_001750"
     CDS             4363..4452
                     /gene="tisB"
                     /locus_tag="ACHOTW_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFP9843873.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(4576..4650)
                     /gene="ysdE"
                     /locus_tag="ACHOTW_001751"
     CDS             complement(4576..4650)
                     /gene="ysdE"
                     /locus_tag="ACHOTW_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP9843874.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            4732..5916
                     /gene="emrD"
                     /locus_tag="ACHOTW_001752"
     CDS             4732..5916
                     /gene="emrD"
                     /locus_tag="ACHOTW_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP9843875.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(5924..6421)
                     /gene="yidF"
                     /locus_tag="ACHOTW_001753"
     CDS             complement(5924..6421)
                     /gene="yidF"
                     /locus_tag="ACHOTW_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9843876.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(6418..6780)
                     /gene="yidG"
                     /locus_tag="ACHOTW_001754"
     CDS             complement(6418..6780)
                     /gene="yidG"
                     /locus_tag="ACHOTW_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP9843877.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(6770..7117)
                     /gene="yidH"
                     /locus_tag="ACHOTW_001755"
     CDS             complement(6770..7117)
                     /gene="yidH"
                     /locus_tag="ACHOTW_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP9843878.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            7226..7675
                     /gene="yidI"
                     /locus_tag="ACHOTW_001756"
     CDS             7226..7675
                     /gene="yidI"
                     /locus_tag="ACHOTW_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFP9843879.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(7722..9215)
                     /gene="yidJ"
                     /locus_tag="ACHOTW_001757"
     CDS             complement(7722..9215)
                     /gene="yidJ"
                     /locus_tag="ACHOTW_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9843880.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(9212..10927)
                     /gene="yidK"
                     /locus_tag="ACHOTW_001758"
     CDS             complement(9212..10927)
                     /gene="yidK"
                     /locus_tag="ACHOTW_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP9843881.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            11094..11987
                     /gene="yidL"
                     /locus_tag="ACHOTW_001759"
     CDS             11094..11987
                     /gene="yidL"
                     /locus_tag="ACHOTW_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9843882.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(11984..13306)
                     /locus_tag="ACHOTW_001760"
     CDS             complement(11984..13306)
                     /locus_tag="ACHOTW_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFP9843883.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(13306..14922)
                     /locus_tag="ACHOTW_001761"
     CDS             complement(13306..14922)
                     /locus_tag="ACHOTW_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9843884.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            15218..15934
                     /gene="yidP"
                     /locus_tag="ACHOTW_001762"
     CDS             15218..15934
                     /gene="yidP"
                     /locus_tag="ACHOTW_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9843885.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            complement(15931..17592)
                     /gene="yidE"
                     /locus_tag="ACHOTW_001763"
     CDS             complement(15931..17592)
                     /gene="yidE"
                     /locus_tag="ACHOTW_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP9843886.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(17788..18216)
                     /gene="ibpB"
                     /locus_tag="ACHOTW_001764"
     CDS             complement(17788..18216)
                     /gene="ibpB"
                     /locus_tag="ACHOTW_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP9843887.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(18328..18741)
                     /gene="ibpA"
                     /locus_tag="ACHOTW_001765"
     CDS             complement(18328..18741)
                     /gene="ibpA"
                     /locus_tag="ACHOTW_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP9843888.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(18735..18807)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            19047..19379
                     /gene="yidQ"
                     /locus_tag="ACHOTW_001766"
     CDS             19047..19379
                     /gene="yidQ"
                     /locus_tag="ACHOTW_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9843889.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(19381..20595)
                     /gene="yidR"
                     /locus_tag="ACHOTW_001767"
     CDS             complement(19381..20595)
                     /gene="yidR"
                     /locus_tag="ACHOTW_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP9843890.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWPVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            20696..21760
                     /gene="cbrA"
                     /locus_tag="ACHOTW_001768"
     CDS             20696..21760
                     /gene="cbrA"
                     /locus_tag="ACHOTW_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP9843891.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(21757..21870)
                     /locus_tag="ACHOTW_001769"
                     /pseudo
     CDS             complement(21757..21870)
                     /locus_tag="ACHOTW_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418146.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22006..22662
                     /gene="yidX"
                     /locus_tag="ACHOTW_001770"
     CDS             22006..22662
                     /gene="yidX"
                     /locus_tag="ACHOTW_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFP9843892.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(22708..23520)
                     /gene="yidA"
                     /locus_tag="ACHOTW_001771"
     CDS             complement(22708..23520)
                     /gene="yidA"
                     /locus_tag="ACHOTW_001771"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9843893.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(23635..24033)
                     /gene="yidB"
                     /locus_tag="ACHOTW_001772"
     CDS             complement(23635..24033)
                     /gene="yidB"
                     /locus_tag="ACHOTW_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP9843894.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            24151..24249
                     /locus_tag="ACHOTW_001773"
                     /pseudo
     CDS             24151..24249
                     /locus_tag="ACHOTW_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24273..26687)
                     /gene="gyrB"
                     /locus_tag="ACHOTW_001774"
     CDS             complement(24273..26687)
                     /gene="gyrB"
                     /locus_tag="ACHOTW_001774"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9843895.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(26716..27789)
                     /gene="recF"
                     /locus_tag="ACHOTW_001775"
     CDS             complement(26716..27789)
                     /gene="recF"
                     /locus_tag="ACHOTW_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9843896.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(27789..28889)
                     /gene="dnaN"
                     /locus_tag="ACHOTW_001776"
     CDS             complement(27789..28889)
                     /gene="dnaN"
                     /locus_tag="ACHOTW_001776"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9843897.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(28894..30297)
                     /gene="dnaA"
                     /locus_tag="ACHOTW_001777"
     CDS             complement(28894..30297)
                     /gene="dnaA"
                     /locus_tag="ACHOTW_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9843898.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            30594..30674
                     /locus_tag="ACHOTW_001778"
     CDS             30594..30674
                     /locus_tag="ACHOTW_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9843899.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            30904..31044
                     /gene="rpmH"
                     /locus_tag="ACHOTW_001779"
     CDS             30904..31044
                     /gene="rpmH"
                     /locus_tag="ACHOTW_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9843900.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            31061..31420
                     /gene="rnpA"
                     /locus_tag="ACHOTW_001780"
     CDS             31061..31420
                     /gene="rnpA"
                     /locus_tag="ACHOTW_001780"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9843901.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            31384..31641
                     /gene="yidD"
                     /locus_tag="ACHOTW_001781"
     CDS             31384..31641
                     /gene="yidD"
                     /locus_tag="ACHOTW_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9843902.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            31644..33290
                     /gene="yidC"
                     /locus_tag="ACHOTW_001782"
     CDS             31644..33290
                     /gene="yidC"
                     /locus_tag="ACHOTW_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9843903.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            33396..34760
                     /gene="mnmE"
                     /locus_tag="ACHOTW_001783"
     CDS             33396..34760
                     /gene="mnmE"
                     /locus_tag="ACHOTW_001783"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9843904.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(34910..35562)
                     /locus_tag="ACHOTW_001784"
                     /pseudo
     CDS             complement(34910..35562)
                     /locus_tag="ACHOTW_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228226.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
BASE COUNT         8664 a         9330 c         8864 g         8704 t
ORIGIN      
        1 tttacacaca gacggttaac aatacgtgtt caccttaatg ataaacgcgc tttatcaaga
       61 aaaaatcata aatcagcagt tgttgcggca ccggaaaatg taaaatcgat tttatcgctt
      121 ttggattttt cttcgtcgac actaccggta gtaaaataac aagagcactg aagcttcact
      181 tcatttccaa cattattaac cgccatcaaa tacatcttct gactaaaccc atttatagta
      241 aaatcaattt ctttaagata cttcccagtc ccaaaaaagg catttcctga cttttttaaa
      301 cccgatgttg atgaaatggt accattcaaa ctctttgaga gtaactcgct aacatcttga
      361 gatgtatttt ttaaaaaact ctgatagcct tgcaaaggag aataataaaa acatgtaatc
      421 ccctgagggt tggataagca agcggttatt tcatcaatct tatcatccgt aatattaccg
      481 tttgattgtt ttaataattc ccttacctga gcacaatcat catcattaaa gcaaaccagt
      541 tcaaccattt gttcaagtgt gttttgacta ttctttggat ctttttcaat tatttcaatt
      601 ttgtctgtaa atattttaag aatatcttca cttaactcaa caccgcagca agttatttta
      661 gtagcaccct tttgttgact ttgttctttt atagttcaat gagcatccat aatcatggaa
      721 tcaaaaaaaa catcagttct tttaaaactt tcctgaacag gtacataccc ttcatctttt
      781 ttaattcgca gttttaataa atcatatata ttacttaaaa ttcctgcagg aatttcgtaa
      841 ttaaaatccg tattcttcat aacaatacta ttaactaatt tcttcctcaa aaccgtgtca
      901 gcggaatgtg tttctaacgc agaagttttt tcagacaacg atttaataat agtcgctaca
      961 gccactcctg aatatggaat ttcttttcgt gaaggtttat ctctgccaac cagtgtaata
     1021 ttaaaagact tatcacctgt ttgtttgatt tcgatatatt catcctctcc agttgccgcc
     1081 acccaggtac gattaccatt attttccgtg cctggcatag ggtggaaata gtcaagaata
     1141 tcggtgttaa tactccgaga accggacatg atgtttagta ttaagcgctt tatttcctgc
     1201 aacatccatt ttgcgaaatt actgccaggt gaatattttt tctcgttatt tttaatatca
     1261 agctcagcag cgacatttct atttttatcc aaaaaggtgg aagggcagag atttgccatc
     1321 attattccgc tgtttatagg acttgccata atatacctca cgatgaacca ttaattaata
     1381 gaatgctggt catgaccact ggtatagaca gcaatcagaa tttaacacca tcgctgaaaa
     1441 ttttagccct aactgtcccc cccaggttac cggtgctgcg acttaaatta acgtcctggg
     1501 ctaataaatt gtgcctgaga tggtctctgg cacaatttca ccctatatat ctgcacggat
     1561 ctatcaaaaa tcgctcatca gtaactcccc cgagcggttt aatgtcgctc cttactgaaa
     1621 aaacactgca atcttgttaa acatcgtcgg atcggactga ttacgctgca ctttcaccac
     1681 atcttccagc ttatcgattt ggcttatcat ctgctccaga cgctggtcgt cattgaccag
     1741 tagccagata tggcttttgt cgctgtcctg aatcggcaga caaagaatgc cttcaacgtt
     1801 aaaagcgcgg cgggcaaaaa ggccacaaac gtgggtcatt acgcccggat ggttgcgaac
     1861 ggtgagttcc agaatcacgt tgtcatgagt tgtgttttgc atggcttatt cccccaccat
     1921 ttctgtattc gccgcacctg gcggcaccat cgggtacact ttttcttcgg catcaatgcg
     1981 cacatggatc agcgccggac cagggcgatt gatgatttcc tgcaatgcag cctgcggatc
     2041 ggcttcgtta ttcaaatcac aggtttcgag gccgaatccg gcggcaatct gcataaagtt
     2101 gatttttccc ggataggtag cggcaaaaac gccctgcttg tagaacagac tctgttgctg
     2161 atgcaccagc cccagcgctt cgttgttcat cagaatgatt ttaacatcca gctggttttc
     2221 gctggcggtc gccatctcct gaatattcat catcaggctg ccatcgccgg agaaacacaa
     2281 cactttgcga tccgggttcg ccagcgccgc gccaatcgcc gcaggcaggc caaaacccat
     2341 cgtgcccagc ccgccggagg tcagccactg gcgtgggcga ttgagcggat aagcctgcgc
     2401 cgtccacatc tgatgctgac ccacatcggt ggtgataatc gcattgtcat cgacacaggc
     2461 ggcaacggcg ttgatcaggc cgtaatggct taacggatcg cacgctttcg ggattggaca
     2521 cgggaattcg cgctgcaaat ccgctaccaa ctggtgccac tctgcacgcg gttgcgcttc
     2581 cacctgcggg atcagctgcg ccagcacgtc atcaacatcc gcctgaatcg ctacgtgcgg
     2641 ctgcttgatt ttacccagct ctgcgcggtc gatatcgaca tgaataattt tggcattcgg
     2701 gcagaactgc tcggttttgc caatcgcccg gtcatcaaaa cgcgcaccga gcacaatcaa
     2761 caaatccgcc tcctgcaaaa tatagttggt gctgcgcaca ccgtgcatcc ccaacatacc
     2821 cagcgacaac ggatgcgctt ttggcaacat gcccagcgcc attaaagtca ttgtggtagg
     2881 cagctgcgct ttctccgcca gttcacgcac tctcgcaggc gcattgatca caccaccgcc
     2941 cagataaagc accgggcgtt tggcagcatt aatcattgcc gctgcgtcac gaatgctttc
     3001 ttcgctaaag gcgggcgcga cggctttttc tgccaccgcg ggctgtgctt caatctcaaa
     3061 aacggccgtt tgcacatcct taggaatgtc tatccacacc gggccagggc ggcctgattg
     3121 cgcaatacgg aaggcatcgc tcatgacctg cgggagttct tcgatatgtc tgaccagata
     3181 gttgtgtttg gtgatgggga tagagatgcc gtaggtgtcg acttcctgga aggcgtcagt
     3241 gccgatcatt gaggcgggaa cctgaccagt gatgcaaatc agcgggatgg agtccagccg
     3301 cgcatcagca atggcggtca ccaggttagt cgcccccggt ccgctacagg ccatacagac
     3361 cgccggttta ccgtcggtgc gcgccattcc ctgagcgata aaccccgcgc cctgttcatg
     3421 gcgagccaga atatggcgga tttgcgtgct ttggcttaag gcatcgtaaa caggcaggat
     3481 agaaccgccc ggaatgcccg tcacaatctt aatgccctgc tgttccagga aatgaacgat
     3541 aaattctgcg ccggtaaagc gcttacgcgt cgatgatgtg cccgaacttg ccatgctcca
     3601 gtccttttct tctgggccga ctttccggga ggtgcttaaa cgaaaaaccc cgcccggttt
     3661 gcgccggcgg ggttttggaa tcgtgtgttg ttccagtccc tacggcgcat tgccgacgac
     3721 caccaccaca cgcacgacga ccactgcggc ggatggcgca gttggtagta gttttgcgtt
     3781 gagcatggaa gtagccattg gggaccttgt tggttttgtg tttaacaata tttatacaag
     3841 cacagcttta caggggagac aatggaaaat ttttcagcaa gggaaaattg aggggttgat
     3901 cacgttttgt actgtactgc aggtaacaaa aaaccccgcc ggagcgaggt ttcgtcagcc
     3961 gcccgcggct ggtaaccgca aagcacactg tattatgtca acacagaaag tatacgtgtt
     4021 ccgcgcaaaa cgcgcaattt cggcacgaat tttgacgtat ttagtgcata gttgagtatc
     4081 gatcacagtt tgcgttttgt ccaaatatta ctgtttattt atacagcaag cttctataat
     4141 atcactgtac gcaatgtgtt atgcgggggc cgcatcgtta cccggcgcac taagtcctgg
     4201 ctgaaacggg tggtgccgtc agcgccttaa ccccgcgtga gcacactgtg ttatgtcaac
     4261 gagcacaacg tttctccttg agataccgcg tgcacaacag ctggcaacag gcagcggaaa
     4321 ggtacgtcag ctggcagtgc tcctgaacca caggagacgc gtatgagcct ggtggatatc
     4381 gccattctta tcctcaaact cattgttgca gcactgcaac tgcttgatgc tgttctgaaa
     4441 tacctgaagt aattcagatt caagtcgcac taaaggggag cgggtaaccg ctcccatttt
     4501 atatttagcg tgtgggttgg tgtcggatgc gatgctgacg catcttatcc gacctaccat
     4561 ccctcccggc aacatttatt gccgcttttg tttacatatt ctgccgctaa acaattcccc
     4621 attcctggcg tatatctggc taacatttat caatatgata gattcctctc ccgcatttat
     4681 gggaatgcgt agtgacttat tctaattatt tttataaaag catccgtgat aatgaaaagg
     4741 catagaaaca tcaatttgtt attgatgttg gtattactcg tggccgtcgg tcagatggcg
     4801 caaaccattt atattccagc tattgccgat atggcgcgcg atctcaacgt tcgtgaaggg
     4861 gcggtgcaga gcgtaatggg cgcttatctg ctgacttacg gtgtctcaca gctgttttat
     4921 ggcccgattt ccgaccgtgt gggtcgccga ccggtgatcc tcgtcggaat gtccattttt
     4981 atgctggcaa cgctggtcgc ggtcacgacc tccagtttga cagtattgat tgccgccagc
     5041 gcgatgcagg ggatgggcac cggcgttggc ggcgtaatgg cgcgtacttt gccgcgtgat
     5101 ttatatgaac ggacacagtt gcgccacgct aacagcctgt taaacatggg aattcttgtc
     5161 agtccgttgc tcgcaccgct aatcggcggt ctgctggata cgatgtggaa ctggcgcgcc
     5221 tgttatctct ttttgttggt actttgtgcc ggtgtgacct tcagtatggc ccgctggatg
     5281 ccggaaacgc gtccggtcga cgcaccgcgc acgcgcctgc ttaccagtta taaaacgctt
     5341 ttcggtaaca gcggttttaa ctgttatttg ctgatgctga ttggcggtct ggccgggatt
     5401 gccgcctttg aagcctgctc cggcgtgctg atgggcgcgg tgttagggct gagcagtatg
     5461 acggtcagta ttttgtttat tctgccgatt ccggcggcat tttttggggc atggtttgcc
     5521 ggacgtccta ataaacgctt ctcaacgttg atgtggcagt cggttatctg ctgcctgctg
     5581 gctggcttac tgatgtggat ccccgactgg tttggcgtga tgaatgtctg gacgctgctc
     5641 gttcccgccg cgctgttctt tttcggtgcc gggatgctgt ttccgctggc gaccagcggc
     5701 gcgatggagc cgttcccttt cctggcgggc acggctggcg cgctggtcgg cggtctacaa
     5761 aacattggtt ccggcgtgct ggcgtcgctc tctgcgatgt tgccgcaaac cggtcagggc
     5821 agcctggggt tgttgatgac cttaatggga ttgttgatcg tgctgtgctg gctaccgctg
     5881 gcgacgcgga tgtcgcatca ggggcagccc gtttaagctc acgtcaccgc agcatcgtca
     5941 tcagctccat gggagaacga tgctgcttta tcagatcacg catcacccgc atatgcggcg
     6001 cagagtaaga ataaaacgcc tgatagcccg cacaaagcac gctgtctggc gtctcctccc
     6061 gagcgtgcgg acacagcaga tgccagtcgc acgcctggca gccacggcgc ggttgcgggt
     6121 tttcgctgcc acaccatttt tgtaatgtgg cgtcaaaaag ctcaatcgag atacgactga
     6181 tatctgcccg cacccaacac tcaaacaggg caattaaaaa tttcccccac agctctgctg
     6241 tcaccgaagc ggccgtcata tcgccccggt catcaaattc aagatgggga ataagctgta
     6301 cggagcggat gccccgttgt ttgaaattgt gataaaaatc ggcaggttgc agagcatcct
     6361 tgtactctac aaccagttta tgcctgtcct cttcagcatc aatgacctga cttcctgtca
     6421 tgcgactctc tcaataaata cgataagttg atgtatatgc gttacagcaa acagtattgc
     6481 gagagataac accgcgaggg agatcaaaaa tttgtcacgg actacgtgaa attgggaaaa
     6541 atcgctattc gtgacatcca ttaaattgcg attacgagtg tagtgccaga ggatcagcgc
     6601 cacgatggcg aggatgccaa tcgaaatcca gaataacatg cccgcctggt gccagttgtg
     6661 tttgatagcc aacgccatca acgcgccgta gcccagcatg gtacgaaacc acgccagtga
     6721 tgtacgctcc ggctgcagcc ctgggtcggc aatgcgtctg gctttgcggc tatccggcat
     6781 acaacaccag tcccataacg atcactgcta cgaccatcaa tattaagctg atgattaata
     6841 agctgttggt gtagggcagg tcctctttca gacgcatcgc tttttcattg cgtaaccagc
     6901 gcaagtagcc atacatcgct aacccgccgg aaaacagaca caacaacaac gccagcagtt
     6961 cgcgaatgac gggcgtggcg aaatctggcg caagttgatc aagcccaacc cccgccgcca
     7021 gaaatcctag tgcagtacgg atccacgcca gaaaagtacg ctcattagcc agcgagaagc
     7081 ggtaatcagg cgcttctccg aggcgggaaa ttttcattgt tgctcctttg caatttcact
     7141 aacgctctac ctttagcggt tttttttaag aatttcgtcc gcccgtttcc tgatatgctt
     7201 ttgccatccg ctacaaggag gtcagatggg tatcatagcg caaaataaaa tcagttcatt
     7261 gggcatgcta ttcggtgcca tcgctttaat gatggggatt atccactttt cattcggccc
     7321 tttttccgca ccaccgccaa cgcttgaaag catcgtggcg gacaaaacgg ctgaaattaa
     7381 aagaggtctt ctcgccggaa taaaaggcga aaaaattacc accgtggaga gaaaagagga
     7441 cgtggatgtc gataaaatcc tcgatcaaag cggtatcgca ctggccattg ctgcgctact
     7501 ttgcgcattt attggcggta tgcgtaagga aaatcgctgg ggcattcgcg gcgcgcgggt
     7561 ttttggcggc gggacgctgg ctttccatac cttgcttttt ggtatcggca tcgtgtgcag
     7621 cattttgctg atattcctta tcttttcctt cctgactggc ggttcactgg tttaagcagc
     7681 ctgaataatg cccgccgggt gttcatcagc gggcattatc gtcagaactt ctgttttttc
     7741 tcttccacct tcaccccttg tgtcggcagg ccggtgtcat agtcgcgtac cactggcgaa
     7801 taaccatctt gtgggcgtgg acgaaatgcc cccatccagc gcggctgtgc atctttacgc
     7861 cacggacgca ggctccattg gtaactgcgg aacggatcgc gaattttgtc catataatcc
     7921 aataaggcat catgcatttt gctgcgaacg tcggcaaaat gggcatcatc gatcaggtta
     7981 tgcatttcat ttggatcatt acggcgatcg taaagttcat cactggtgaa gaggttgagc
     8041 accagtttaa agtcatccgt cacccagcaa cgcaccggaa taaaaccgcc aaagctgtca
     8101 tgctcaatct cgtagcggtt aaattccacc atcacgccgc gtggctcttt cactgcaagg
     8161 atattttccc ccggcagaat ctctggtttt tcaatatctg ccagcgccat cattgtcggc
     8221 agtaaatcga tatgactgac tggcgtatcg acctgtcgcc gctccccttg cggcgaacgg
     8281 atgatcagcg gaatgcgggt gatgtcgtca tacatcgccg cccctttact gatcagctta
     8341 tgtgcgccca tcatttcgcc gtgatcggag gtataaataa cccacgtatt ttcacgttgc
     8401 tctggcttta aggcattgat gacccgtccg atttggtcat caacaaagtc attacaggca
     8461 aaatagagcg gatggtgata aagcccgtca tcaccgactg gcgatggcat cgcctgcgcc
     8521 cataagcggt gatgttctgg tttgttcgcc aggtcatcct gtgctttctc gcccagctcg
     8581 tagtaaaaat cagcgtattt ctctaaatac tccaccggac aggtgaacgg gtgatgcggc
     8641 tcatcatacg aaaccaccat caggaagggt tcctcggcgc gcgcgggctg ttgcagaaaa
     8701 tccaccgctc gattgctgat gcggtgcgcc caggtgaagg tttcgtcaat atggttcgcc
     8761 tgtaaatctt cgacgctgtt taggccattg cgccacaggc taatctcttt ttccgtcagt
     8821 tcgctaagat agttcgcccc atcgaaccag taatcagcgt cccactccgg cggacactcg
     8881 ccagtgccga aatagtcatg accgtcgaga tgccatttgc cgatgtaaca ggtgtgatag
     8941 ccggcatcct taaagtagcg ccccatagtg gagatgtttt tacccggcgc gacgttgttg
     9001 gtccacggcc cggactggtt agcgtagata ccggtaaaca gcccggcgcg cgccggtgta
     9061 caaaccggtg aacaggtgta ggcggaatta aagcgaatac cttccgccgc cagactatca
     9121 atattttgcg tattcagcgg tttaccgcta tagcaaccga ccatattggg ggcctgggta
     9181 tcggtcatga tgaacagaaa attggggcgt ttcataggct atccttcgcg tcgggagtaa
     9241 aggttacggc tgggtcgtga cgatgccgcc agctgtcaaa aatcaggtag acaatcacta
     9301 cggcggcaat gaaataactg atcatcgcta accagcgcgt accgtagccg ccgaattcag
     9361 ccagcccggc atagacgcca atcatcgcga acaggatgcc aattgacgcg atcttgacgt
     9421 ttttccacgg tttcatgtcc accgcaaacg catctttgaa ggtgaacggc gtggcgcgcg
     9481 gtttgataaa accgatcacc agcatcacga ccacgttgat gcagaacgta caggccagca
     9541 catagaggaa atggaagtcg aacttcacca gatagttgat ggtgatgtag ctgattatgc
     9601 caatccccat cgccactttt gccgccagcg ccgggatgcg caggaagaaa aagcccataa
     9661 tgatgatggt aaccagcggc acgttgtaaa tgccgttgag ctgtttcatc cagctataca
     9721 gcccctgcgg cgcgttggcg atccacggcg ctaccagcac cgaaacgata gcgataaaga
     9781 aaccaaattt gcgcccgacg gtgaccagct gctgcggctc ggcattctgg ttaatgatgc
     9841 gacggtaaat acccatactg aataacgtac tggcgctatt cagaaagccg ttgaaggtac
     9901 tgatcactgc accaaataac accgcgccga agaaacccac cagtggcact ggcagaacgt
     9961 tattgaccag cgtcgggtag gccatgtcgg ctttcggtag atcctgatac agatgaaacg
    10021 caatcaaccc tggcagcacc agtaccagcg ggtcgagcat tttcagcacc gccgttaaca
    10081 gcgccccctt ttgcccttcc gccaggcttt ttgacgccag cgtgcgttgc acgatgccct
    10141 gatttgtaca ccagtaaaag gtgttcacca gaattaaacc ggtaaatgcc gcgccaatcg
    10201 gcaagggatc ggtcgggcca ccgactgagt ttaatttctc ggcgtgaacg gtggtaattt
    10261 gctcaatgcc ctgcataaag ctgcccttgc ccatcgcgat caagccaaat accggcacca
    10321 tcaacccgcc gataaccagc ccaataccgt tgatggagtc cgccactgcc attgcgcgca
    10381 gtccgccgat caccgcatac aaaattcccg ccagaccaag caaaattacc agtagccaga
    10441 tagccgcacc gtgagaaatc tgtagcgatt ccccgacgtg aaacaggctg ttgagcgcca
    10501 acgcgccgga gtagagaaca atcggcagaa agcagacgcc ggtggcaatg aggaagcaga
    10561 agtcgatgat catacgcgtc gttttatcat aacgttcctc cagaaaatcg gggatggtgg
    10621 caatgccgcg cttcagatag cgcggtaaaa agattagcgc gaggaagatc agcgtcaccg
    10681 ctgaagtcac ttcccagccc atcaccgaca tgccgctttt gtaggcctgc ccggaaagac
    10741 caaccagttg ttccgtggaa aggttggtta acattaacga agcggcaata accggcgctt
    10801 ttagtgaacg tccggcaaga aaatagcctt gttgcgatcc ggtatctgtt ttgcgaacct
    10861 tccaccaggt gatgatcgcc accagcagcg taaaaccgac aaaactcaag atttgtaacg
    10921 aattcatctc atagccctta tttatgttta tgtcccggac gcatgaatat agggagaggt
    10981 aaaatgcgtc gggtcaggca gtcagattct taaaatacaa acgtcgtatc cctgaacgga
    11041 ttgttttctg ttaagttcag gttgtgagca taattcgccg gagggatatt aaaatgaatg
    11101 gaaaattgca aacttcagat gtaaaaaacg aaactccata caatattcct ttattgatta
    11161 acgaaaatgt gatctccagc ggaatttctc tgatctcgct ctggcatacc tacgccgacg
    11221 agcattaccg ggtgatctgg ccacgggaca agaagaaacc gcttatcgcc aactcatggg
    11281 ttgcggtgta taccgtacaa ggatgtggga agattctttt aaagaatggc gaacaaataa
    11341 cactgcatgg caactgtatt atatttttaa agccaatgga tattcactct tatcactgtg
    11401 aaggtttagt ctgggaacaa tactggatgg aatttacccc caccagtatg atggatattc
    11461 ccatcggtca gcaaagcgtt atttacaatg gcgaaattta taatcaggaa ctcaccgaag
    11521 ttgctgagtt aataacttca ccagaagcaa taaaaaataa tctggcagtc gcttttctga
    11581 cgaaaattat ttatcagtgg atttgtctta tgtacgcaga cggtaaaaaa gatccacaac
    11641 ggcggcaaat tgaaaaatta attgccactt tacatgccag tctgcaacaa cgctggagcg
    11701 tagccgatat ggctgccacg atcccctgta gcgaagcctg gttgcgtcgt ctgtttttac
    11761 gctataccgg aaagacgccg aaagaatatt acctcgatgc gcgtctggat ctggcgctat
    11821 cgctattaaa gcaacaagga aactcggttg gcgaagtcgc tgatacgctc aacttcttcg
    11881 actcctttca tttcagcaaa gcctttaaac ataaatttgg ttatgcgcct tcagccgtgc
    11941 taaagaatac ggaccagcac ccaacggatg ccagtccaca caattaacgt aactctggcc
    12001 agtacgcttt gttggcctcc agcaactcat ccagaatagc tttggcgacc gaggcgctcg
    12061 gtaccgtttt cgacaacgta atcgcctgcc acaggtgctg atatgaacgc tgttcccagg
    12121 cgtccacgac cagtttttcc accgccactt gctgactcat taaccctttc tgaaactgcg
    12181 ggatatcgcc gaccaccagt ggttctggtc cgttgtgccc gaccagacac gggatctcga
    12241 ccatagcttc atcatcaaag ttatggattg ccccgttgtt aggcacaatc agcaacatcc
    12301 gctcctgagt gttgaaggca attgccgccg ccagatcgac gatgtatgac gcatgttcgt
    12361 caatttccag cttgcccgcc gctgaatttc ctgccgccgt aatggcgcgg caagcatcga
    12421 aaacctgttt ctcgcgatgt tccattacct cattcgcgcg agtatgttca gggttggaat
    12481 gctgaacgac ataatccggg aagagataat atttcagata ggtgttcggc aatgtatccg
    12541 ggtccagtgc ctggacatcc cgcgcttttg cataggtgtc gttccagctc gcttcagtat
    12601 gtgtatcttg ctgcggtgga acataaccgt gcttagagac atattgccgc agctggtgca
    12661 tcaggtcgtt gccctgcaaa tcttcaattg atgtccacca gccaaagtgg ttcaggccgt
    12721 agtagcgcac gcgcatctgt ttgcgatctt gcagcccaac aatttgcgcc atccggcttt
    12781 caataccgat tggcatgtca cagatgttga ggattttcgc attcgggcgc agacgacgcg
    12841 tggcttctgc aacaattgct gccgggttgg agtagttgag catccaggcg tttggtgaat
    12901 atttttccat ataatccacc agttccagga cgccgccaat ggaacgcatg ccgtacgcta
    12961 ttccgccggg tccgcaagtt tcctgaccaa caacgccgtg gcgcagcggg attttttcat
    13021 ccagttcgcg catcgggtat ttgccgacgc ggatgtgcgc catgacaaaa tcaacgtcgc
    13081 tgaacgccac ttcaggatcg gtggtgtaac taaacgcaat atccggcgct ttttctttaa
    13141 ggatgacttt acaggcttcg gcaatcactt cctgccgcga accatcgtta tcataaaatt
    13201 tcagtgcacg aagcgggaaa cggtcctgat tcgccaggag catcaacacg atgcctggcg
    13261 taaaggtgct tccaccgcct gcgacaacca ctgagaattt ggtcattata ctgcctccgt
    13321 aatggcaaca ttttctgctg attgatgaga attaattaag ctatcgagct gttcacgcag
    13381 ctgggataca tgcagaccaa tgatcacctg aatggcatcg ccactacgga agacgccgtg
    13441 cgctcccagc tttttaaaga cttcgtcatc cagcgtttgt gacatgtcat gcagtgcaat
    13501 gcgtagacgc gtcgcacaat tgttaatgct ggagatattg ccgaccccgc ccagggcttg
    13561 caggataccg gcagcctgat ccagctcttt ttttggctct gccgcggtgg tttggcctcg
    13621 cgaggctttg tattcggctt ttgagtagag tttcacttcc gcatcttcac gtcccggcgt
    13681 gcacatgttg aattgcagaa tcagggtgcg gaaaaccacg aagtacagca gggtaaagca
    13741 caacccaatg gcgatttggg tcagcatcat atccgcgtgg ttgctgaaca tcgggatcca
    13801 gttttgcggt aaaacctggt caatcagacc tccgcccatg ttgcccacca caccaaagag
    13861 atacatcacg gtcgacattg aggccgcaag cacagcgtgt accgcaaaca gcaacggtga
    13921 aatgaacagg aaggtaaatt ccagcggttc ggtaattccc accagcatgg cggttaaggt
    13981 tgcgggaatc agcaaacccg ccacttttac ccgattttcc ggtgcggcag tgaagtacat
    14041 cgctaaagaa atgcccacgg caccaaagat ttttgagtta ccgtgcaggg caaaacctcc
    14101 ttccgggaac aacgatttca gcggctcggc gctcaaactg aactcttgca gatgctgcgc
    14161 ccagtacatc tgaattccgc cttcaacagc tgccggacca aagataaacg gtccgtagat
    14221 gaagtggtgt aaaccggttg ggatcagaat acgttcgagg aaggtgtaaa cccacacccc
    14281 aagcgcaccc gccgaacgca ggaacgcttg cagagattca atccccattt gtacttttgg
    14341 ccagccgagc aatgtcagcc aggcacaggg gatcatcacc aggaaggcga taatcaccac
    14401 ataagacgtc ccctggaaaa tgccgagaaa aaccggcagt tttttatcga acagacggtt
    14461 atgcagcgcc gtcacaatgc cggaaatgat aattgcgccg ataatgctgg tatcgagggt
    14521 tttaatcccg gccatcattg tcagaccgct acctgccacc gcgtcctgag tgaaatcgac
    14581 gccgaagtag cttccccagg tcattcccat cgcattgatg aaatagttcc aggtcaggaa
    14641 actcaccatc accgccagac aagcacgccc ctgcgcttgc ttagcaaggc caatgggtaa
    14701 accgacagca aaaatcagcg gcatattacg gaataccgtc caaccgccct cttcaataat
    14761 gtgtacgatt tgcgcgaata aactgttcgg atcggtcagt gattccccga caaacatcgg
    14821 gttttgcagc aagatggcaa gacccaccac aatcccggcg aagggaaaca gcagcactgg
    14881 cgtgaacatc gcgccgccaa agcgttgaat ttgactgagc atttccacat cctcttttct
    14941 caattctgta agtaggaagc ctgattggtt atgcggcagg cctggagcga ggataggaag
    15001 atgcgatcgg cagaacatga cgacccaatg ggattcgtga tcgctttcat gcttttcgtt
    15061 gggcggtaga gatctacttt ttcgcgaaaa agtagatctc tgtgagcact ggtacacagc
    15121 atcgaacaca tctttaaaaa aaagatgttt tttcaattga ttaagcagaa cttgtgggcg
    15181 cattacccgg gcttgcaggc aaaaaagaga tctagagatg atctacaaaa gcattgcgga
    15241 gcggttaaga attcgactta attccgcgga tttcacgcta aacagccttc ttcccggtga
    15301 aaaaaaactg gcggaagagt ttgcggtatc gcggatgacc atccgtaaag cgattgacct
    15361 gctggtagcg tgggggctgg tggtccgccg ccacggcagc ggcacttacc tggtgcgcaa
    15421 agatgtgctg catcaaaccg ccagcctgac cgggctggtg gaggtgttaa aacggcaggg
    15481 aaaaacagtc accagccagg tgctgatttt tgaaatcatg cctgcgcctc cggcaattgc
    15541 cagccagttg cggattcaaa tcaacgagca gatctacttc tcccgtcgcg ttcgttttgt
    15601 ggaagggaaa ccgctgatgc tggaagacag ctatatgccg gtaaaactgt tccgtaacct
    15661 ttcgctgcaa catctggaag ggtcgaagtt tgaatatatt gaacaagagt gcgggatttt
    15721 gattggcggt aattatgaaa gcctgacgcc ggtgctcgcc gatagactgc tggcgcggca
    15781 aatgaaggta gcggaacaca cgccactgct gcggatcacc tcgttgtcat atagcgagag
    15841 cggggagttt ttgaattatt cagtgatgtt cagaaatgcc agcgaatacc aggtggagta
    15901 ccatttacgg cgactccacc cggacaagag ttaaccgata ctccagaaga gcaccgccag
    15961 taattggggg gtgataatgc gcaggaacat cactaacgga tagacagtgg cgtaagagag
    16021 cgccgccgca ccgctggttg gatgaagatt attagcaaac gccagcgccg gaggatcggt
    16081 catggaacct gccagcatcc cgcacatggt caggtaattc attttggcta acatccgcgc
    16141 cagaatgcca acagtaatca gcggaacggc ggtgatcagg gcaccataac caatccagct
    16201 tagcccttcg ccattgacca gggtattcac aaaatcccca ccagatttca gaccaacgac
    16261 cgagaggaac agcacgatcc ccagttcccg cagcgcgagg ttggcacttg gcggcataaa
    16321 ccagtacagc ttgccgatac tgccgatacg cccgaggatc aacgccataa tcagcggacc
    16381 gcccgccagc cccagtttca acgcggccgg gaatcctggc acaaagacgg gaatagaacc
    16441 taacaatacg ccaagcccga tgccaataaa cactggcagc atctgaacct gttgcagttt
    16501 ttgctgcgca ttccccagca cattggcaac ggcatcaatt gcggacggac gccccaccag
    16561 gttgaggata tcgccgaact gcaggctgat atcgccactg gcgaccagtt cgaccccggc
    16621 acggttcagg cgcgagataa caacgtcata gcgttcttta aaatgcaggt cgcgaatacg
    16681 cttaccaagc acgttttcat tggtgaccac cacacgctcg acgcgcaaat cagtgccttt
    16741 cgtggacagc gaagtatcga cctcctgacc aatcaccagt tgcgcattat gtaaatccgc
    16801 tggctgaccc accagatgca gcaaatcgcc cagttggata atggtatctg gcgaaggaac
    16861 ttttagggtt tcttcgcgtt tcagacgcga gcagataatt ttgtcgccgt tgagaatcgg
    16921 tacatcttta atggctaaat catgcaggtt agggttctca acgcgaatat tgatagtctt
    16981 gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc tgggcttctg tctcgacatt
    17041 gacgcggaaa ataacccgca acatccacat ggtgaacaat atcccgcaaa tgccgaatgg
    17101 atacgccatc gcgtaactca tccccatctg atcgaccatt tccattggtg tacccaggtc
    17161 gcgcaaaatc tgctgccctg cccccagcgc tggcgtattg gttaccgcac cggagaaaat
    17221 ccccagcact accggcagtg gaatatcaaa cagtttatgc aggatggcgg taaccagacc
    17281 accgatgatg acgatcagaa cagcaaacag gttgaggcgt aatccggaga cgcgcaatga
    17341 ggcaaagaag cccggcccta cctgaatccc gatggtataa acgaacagga tcaggccaaa
    17401 ttcctgaata acatgcagca tatcgctact taatgtcatc cccgcctgag aaacaaaatg
    17461 gccgacgatg atcccaccaa acagcacgcc gccaatacct aatcctatgc cgcgaaattt
    17521 gacgttgccg ataaacaaac cgacgactgc caccaaagcc aaaatactga ccgttaatgc
    17581 tatatcactc atcgtccatt tctcttgaga taacatttta attgctacgg attatggctg
    17641 aatcggtgaa gcagatttgc cttatagcgc acattatcac gggaaagaac gtgccgaaat
    17701 atcttaaaca gtcgaaattt aacgcattgt tgaagaagaa agacaaaaaa agccccacca
    17761 gaatggcggg gcaaagagaa tagctagtta gctatttaac gcgggacgtt cgctgatagc
    17821 gatacgctgc gctgcgatgg gttcaggctc attacgaatt aaatcaatat gcagtaaacc
    17881 gtttacgaag gttgcgccag agacttccat attttcagcc agcgtaaagc tcaggctaaa
    17941 tggctgattc ataagccctt gatgcagcca ttttttctct tcttttggct gctccggcgt
    18001 gccttttacg ctcaggcgcg taccttccag ttgaatctct aaatcttcct gacggaaacc
    18061 tgccagcgca agggtaatgc ggtagtggtt atcgtcgctt ttctcaatgt tgtacggcgg
    18121 gaagctctga ctttcaccgg cgttttgcag tgcgttggcc agtttgtcaa aaccgatcca
    18181 ttgacgcatc agtggggata aatcgaagtt acgcatagtc atttctcctt ctaagaagcg
    18241 agtaagtacc tgcaaatccg aagattcgca tatgctccct gacggcgagc atggagatgt
    18301 caggccgcgc aaggcggcct taggaaatta gttgatttcg atacggcgcg gttttttcgc
    18361 ttccggaatc acgcgttcga gatcgatata cagcaaacca tttactaggt tagcaccacg
    18421 aacatgaatg ttctcagcta actggaattt gcgttcaaag ttgcgttcag cgatgccctg
    18481 gtacagatag gtgcgctctt tttgttcgtc ggcgtgagca cctttcacca ccagcagatt
    18541 atcctgggcg gtaatttcca gttcgctctc agcaaaacca gccacagcga tagcaatgcg
    18601 gtaatggttt tcgtctacca gttcaacgtt atacggaggg tagccgccat tactctggct
    18661 ctggttgttt tctaagtggt taaacaaacg gtcaaatcca atagcagaac ggtaaagcgg
    18721 ggataaatca aagttacgca taatcaatag ctcctgaaat cagcgagaat gtaagacctt
    18781 ccacaatgga caggtcaggt agccagaaca cccatcaggc gcgtcctcat cggctacgat
    18841 gtaaaaatgg gtctggaaat gaacttttca agccttatca gcggactttt ttttgcagtt
    18901 tatggtctat tgcatagact gagggggcag cacgaaacgt taaagtgcga tagccgccac
    18961 agagcgacgt aatgatgatg ttattttcag caagggatcg ctataactca tcaggccaac
    19021 acccacgatc aacagatgaa tttattatga taagaaatgt gttgttagcg ttcatgatat
    19081 gcagcggaat ggcattactc ggaggatgct ccagcgtgat gtcccatacc ggagggaaag
    19141 aaggaacgta tcccggcacc cgcgccagcg ccaccatgat tggcgatgat gaaaccaact
    19201 ggggcactaa gtcgctggcg attctcgata tgccgtttac cgctgtgatg gacacgcttt
    19261 tattaccgtg ggatgtgttc cgcaaagaca gctcggtgcg ctcacgcgtt gaaaaaagcg
    19321 aagccaacgc gcaagccacc aatgcggtta ttccaccggc cagaatgcct gataactgat
    19381 ttagcgatca gtttcggtga tccacagctg gccgccttcc atccacgcca gccactgacc
    19441 atccggcgag aagaccacgg cgtccgcaga aggtggattg gcgtggtttt cggttaaata
    19501 ctcaacctcg ccgctttgcg catgggcaca agcaattcga ttacccagca caaagcccaa
    19561 ccattctcct gacggatgcc agttaaatgc agactgaata tccgttttgt tatgggttaa
    19621 ctggcgcggc tcgccgccct gtggcgagat aagccacagt tgcacaatgc cgttatcatc
    19681 tcgcattaaa aacgcgattt gcgtaccctg cggattacag cgcacccagt ggcgcgggac
    19741 gttgactaac cccggataag cccgatggtg ggtaaaggtt aaacgtcgct gcacgatgcc
    19801 acgcggtggc gcgggcaggg ttgtttccgt tccacttaac ggcgcatcac ctgctgcttt
    19861 ccagccagct tcatcttgcg gtaactcaac gataaacagc tccggcactt tctcgccctt
    19921 tggcgaaagt gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata
    19981 agcacgattg atttcatcgc tgccaggctg cggcgtgggc gtggttttac tcaccagcac
    20041 gcaccagtgg ctaccgctgt attcacgcgg atgctgcttt tgtacgttga ccgggccaaa
    20101 cggtgcagca accccgacgt ttcgcaaatc cagcgccgaa tcgaactgat gcattacatg
    20161 gtcgttatag gtaaaactca ccatttcacc gttcgggcta aagacatgca catggctgcc
    20221 gccacgcagc gcccctgatg tgtacggaac ggtaatatcc attgcatcga gattgttcat
    20281 cttgccgcct tcaacaatca cgccgcgacg gtgatggaaa tcgtaatgcc atgtttcatg
    20341 aggattttca ggtccgtgga taaagacata tttctctgac tttggatgaa cggtcaccac
    20401 gccgacgtgc gcgccctgtg acacgcgata gataacctcg acctcgccgg tatggatatt
    20461 cacacgctca atggtctcgc cggtaaacga cgcgccagaa ggacgcacgt caaataccgg
    20521 ccactggctg tcgggcgtcc aggtattggt attggtgagc aggtgattac ggggagcaaa
    20581 ggttatctgt ttcatggcgc ggtcctgata caaaacggtt cccgccatca tacttcacaa
    20641 ggacttcact ttcagacaat agtcttttgc catcgtgatg aatgtgagtg aaaagatgga
    20701 acatttcgac gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt
    20761 agccggcaaa atgcaggtga tcgcgctgga taaaaagcac cagcatggta ctgaaggttt
    20821 cagcaagcct tgtggcggtc tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg
    20881 actgacgctt cctgtcgatg tgatcgccaa tccacagatt ttcagcgtca aaactgtcga
    20941 cgtcgccgca tcgctcacgc gtaactacca gcgaagctat atcaatatta atcgccatgc
    21001 tttcgacttg tggatgaaat cgctgatccc cgccagcgtt gaggtttatc acgatagcct
    21061 gtgccggaaa atctggcgtg aggatgataa atggcatgtc atttttcgtg cagacggttg
    21121 ggagcagcat attactgccc gctatctggt cggtgcagat ggtgccaact cgatggtgcg
    21181 gcgatatctc tacccggacc atcagattcg taaatatgtc gctatccagc agtggttcgc
    21241 agagaaacat ccggtgccgt tctactcatg catctttgat aatgcgataa ctgactgtta
    21301 ctcatggagt atcagcaaag acggttattt tatctttggc ggtgcctatc caatgaaaga
    21361 cggtcagacg cgtttcacga cgctgaaaga gaaaatgagc gcctttcaat tccagtttgg
    21421 taaggcggta aaaagcgaaa aatgcacggt gctatttccc tcacgctggc aggattttgt
    21481 ctgcggtaag gacaacgcct ttctgattgg tgaagcggcg ggatttatca gcgccagctc
    21541 gctggagggg ataagctatg cgctggatag cgcagagatt ctgcgttcgg tgttactgaa
    21601 gcagccagag aagatcaacg cagcctactg gcacgccacc cgcaaactgc gtttaaaact
    21661 cttcggcaag atagtaaaaa gccgatgcct gaccgcaccg gctttaagaa agtggattat
    21721 gcgcagtggt atggcgcata ttccacagtt gaaagattag ccaacgcgcg tcacatcgcc
    21781 caccagcagg atgtaagaga gcgcgccaat caacgcgacg gcggagatat aaaccagtgc
    21841 aggtgcgaac ccatagcctt gcaacagata ttagtccaca aaaaaacacc tcataagaaa
    21901 aggtcttata tcgggcagaa atctgaaaag ggagatcagg cgctaaacag gagggaaaga
    21961 agagtatgct ttcaacggct tagctactcg tttaaaggat taatcatgaa gttgaatttt
    22021 aagggatttt ttaaggctgc cagtttattc ccactggcgc tgatgctttc aggctgtatc
    22081 tcgtatgctc tggtttccca taccgcaaag ggtagttcag gaaagtatca atcgcagtca
    22141 gacaccatca ctgggctatc gcaggcaaaa gatagtaatg gaacaaaagg ctatgttttt
    22201 gtaggggaat cgctggatta ccttatcact gatggtgccg atgacatcgt taagatgctc
    22261 aatgatccag cacttaaccg gcacaatatt caggttgcca atgacgcaag atttgtttta
    22321 aatgcgggga aaaagaaatt taccggcaca atatcgcttt actactactg gaataacgaa
    22381 gaagaaaagg cactggcaac gcattatggt tttgcctgtg gtgttcaaca ctgtaccagg
    22441 tcactggaaa acctaaaagg cacaatccat gagaaaaata aaaacatgga ttactcaaag
    22501 gtgatggcgt tctaccatcc atttaaagtg cgattttatg aatactattc acccagaggc
    22561 attccggatg gtgtttccgc agcattactg ccagtgactg ttacgctgga catcattact
    22621 gcgccgctgc aatttctggt tgtatatgca gtaaaccaat aatcagtaag cgggcaaacg
    22681 cgtttatgct gtttgcccgc ccacagatta attcagcaca tacttctcaa tagcaaacgc
    22741 cacgccatct tcaaggttgg atttggtgac aaagttcgcc acttctttca ccgaaggaat
    22801 agcattatcc attgccacac cgacgcctgc atattcaatc attgcgatgt cgttttcctg
    22861 atcgccaatc gccatgattt cttccggttt aatacctaac acgtcggcca gtgatttcac
    22921 acccgtacct ttgttaacgc gtttatcaag gatttcgagg aagtacggcg cacttttcag
    22981 cacggtatat ttctctttca cttcctgcgg aatacgcgcg atagcctggt cgaggatggc
    23041 gggttcatca atcatcatca ctttcaggaa ctgggtattg gggtccattt tctccgcttc
    23101 gcagaacacc agcggaatgg tggcaacgaa ggattcatgc accgtgtagt agctgatatc
    23161 acggttggcg gtgtacagcg tggtgcggtc cagggcgtgg aaatgagaac cgacttcgcg
    23221 agagagtttt tccaggaaac gatagtcgtc atagctgaga gcagtttgcg ccacggtgtt
    23281 accatcagcg gccttctgta ccagcgcgcc gttataagta atgcagtagt cgcccggctg
    23341 ttccatatgc agctctttca ggtaattgtg aacacctgca taagggcgac ccgtcgttag
    23401 cacgacattc acgccacggg cgcgagctgc ggcaatcgca tttttaacgg caggtgaaat
    23461 ggtgtgatcg ggcagcagaa gggtgccatc catatcgata gcaatgagtt tgatagccat
    23521 gagttcccca ggtagattgg ttcctgaccc atgctaacgc gattccgctc aaaaatcagt
    23581 acaacacccg agggaaaagg gggatgcaac gcgggttgca tgctccccat ttgcttagcg
    23641 gaagagtttc cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgg
    23701 tgacacttca ccttgcggtg agagcgcatc aataatcttc ggcaattgtt ctgccagtaa
    23761 acaggaagcc gtactggtat ccacgccaag tttttgtcca agatcggata ccgcgtttgt
    23821 gccgagtgct gattccagtt gctcgccgct aactggttga ttgccctgtt gattactcag
    23881 ccaggttgag agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat
    23941 gccaccctgc tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag
    24001 aaaggcgccg acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt
    24061 tgacccaaaa agtatagaac tgcgggatga taattgcgga ttgcagaaat aaaaagggcg
    24121 gagatgatct ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttatcc
    24181 ggcctacaaa aacatgcaaa ttcaatatat tgcaggagtc tcgtaggcct gataagcgta
    24241 gcgcatcagg cacgctcgca ttgttaacgc cattaaatat caatattcgc cgctttcagg
    24301 gcgttctctt caataaacgc acggcgcggt tcaacggcgt cgcccatcag cgtggtgaac
    24361 aactggtcag cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg
    24421 tccatggtgg tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg
    24481 atggagaggc cgcgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct
    24541 accggctgac gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt
    24601 ttctcaccca gcgtgcagat acgacgatat tcgccaccag tgataaactc gtgatccagc
    24661 ggatagtcag tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt
    24721 tcggcattgg tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt
    24781 tcgctgacca gcgcgttcac ccaccgggta acggtctgct catcagagag gtcggcttcc
    24841 gtcagcgtcg gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg
    24901 cggttgatca ttttctgcgt cgcgttgtac tcagacacca gtttctctaa cgcttcgcca
    24961 gccaatgccg gtgcgctggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc
    25021 tggtactgat ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact
    25081 ttgtacagcg gcggctgagc gatgtagacg tgaccgcgtt caacaatttc cggcatctga
    25141 cgatagaaga aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc
    25201 atgatgatga tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca
    25261 caaccaagcg cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc
    25321 gctttctcga cgttgaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta
    25381 cgcccctgct tcgcagagcc gcccgcggag tccccttcca caaggtacag ttcggaaagc
    25441 gccggatcgc gttcctggca gtctgccagt ttgcccggca ggcccgctaa gtcgagcgca
    25501 cctttacggc gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg
    25561 ataattttgc caaccacgat tttcgcgtca gttgggtttt ccagcaggta ttccgccaac
    25621 agttcgttca tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg
    25681 gtctgggagg agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca
    25741 cgcgcatcgt caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg
    25801 taggcattca gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca
    25861 cgctgcggaa tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc
    25921 agcgccactt caacgccgat accgtctttt tcggtggaga agtagaagat atttgggtgg
    25981 atcggcgttt tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg
    26041 aagtggtctt ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg
    26101 aacgacaact cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg
    26161 aaggtttcga ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta
    26221 accgccagcg gtgcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg
    26281 cgctgaataa ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg
    26341 tgcagaccgc cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg
    26401 gtcatgatca cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg
    26461 cgcccgtcat cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta
    26521 cagtgacccg cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc
    26581 agaccggtgc cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc
    26641 agccctttca ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc
    26701 tcatttatac ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt
    26761 ttcgtccgac atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgcgattg
    26821 cgtagctttt aagcggctgg caagcagccc gcgacgctca tcatcaagct cagaggcaaa
    26881 atcatctatc aggtagagac accgccgccc gctttcacgg gtgaggaact ctccttgcgc
    26941 cagacgtaag gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg
    27001 cgcaccgtcg gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag
    27061 ctggcgatcg cgttcaaaat tacgttccag cacctcagca tattctgtct ctttctccca
    27121 gccgcgctgg aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcagc
    27181 catatcggcc gcgataccgg cgctatactc cgcgcgccag gtactgattt gctccgccag
    27241 cgggatcagt tctttatccc acgggcgtag ctgttcgtaa cgtgtcacct ggcgcagcgc
    27301 cgcattgcgc tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccgggttc
    27361 gttgtgaaag catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa
    27421 agtaaaccct tctggcgtta tcagctgcat tggcatcagg tgcgccagtt ccgcgacttt
    27481 atgcccgtca gtaccgtcga tgcggacttt gctgtcgccc tgtttgtctt tggttaagcc
    27541 aatcgccgtc tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaacg cctcctgctc
    27601 gtggcgaatg acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata
    27661 gatggcttcc agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc
    27721 gggagataaa gcgagatccg cggtttcaat gttgcggaaa tcgcggatca acaagcgggt
    27781 gagggacatt acagtctcat tggcatgaca acataagccg cgctctggct ggctgcatct
    27841 tcaatctgca cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc
    27901 gcgttcagaa catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta
    27961 taggtaacgt cgaggatctc ttccgcttct tcctgttccg ggttgttggc ggtgattttc
    28021 agctggtttt cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaattgcc
    28081 gcgcgagcaa acgcctgctt gaggagatcg cagcctgctt ccagatgttt gtccggattc
    28141 ttcggcagaa cgcggcggta atccgggaag cgaccatcca ccagtttaga ggtgaagata
    28201 aagtcgccaa cgtgggcgcg aatattgttg ctgccaatct gcacacgcag cggattatcg
    28261 ccgccgtcga gcatacgcat cagttcaatc acgcctttac gcggcacgat caccgaatgg
    28321 cttggcaaag attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggtcgct
    28381 acggtgcgca gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaacg
    28441 tcctgatgcg ccatagaaaa ctgggtcgct tcaatcagac gcttcatcgt cgcctgcggc
    28501 agggtaaatt cgacttcact ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg
    28561 gtagacagcg aaaaacggct acgcccggag cgcaccagca tccgctcacc ttccagctgc
    28621 acggcaattt ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga
    28681 acggtcgtcg ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc
    28741 tcgagatcag taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg
    28801 agaatcggta gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat
    28861 aaatgctcac gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt
    28921 tgaaaaatct tctttgatat cgtggctctc ttcacgcagc tgctcgatct tccggcaggc
    28981 atgaagcacc gtagtgtggt cacgaccacc aaacgcatcg ccaatctctg gcagactgtg
    29041 gttagtcagc tctttcgcca gcgccatcgc catctgacgc ggacgagcca ccgagcggga
    29101 tcgacgcttg gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg
    29161 aatattgtcg atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg
    29221 cacgaagtcg atagtgatcg cccgtccggt aaagttggcg ttggcaatga cgcggttcag
    29281 cgcgccttcc agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc
    29341 gcctggcaaa cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cgcgggtttc
    29401 taactctggc ggttcgatcg ccacagtcag cccccagccg aagcgggatt tcaaacgatc
    29461 ctcaacgccg ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc
    29521 ttccagcagg gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa
    29581 aaactgaata tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc
    29641 gatcgcgttg ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata
    29701 aaccactttg gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg
    29761 agttttaccc agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt
    29821 atctgccacc tggcgagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt
    29881 atcaaacgtg tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac
    29941 gttatcccaa cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg
    30001 tgcaggggcc gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccgatttggt
    30061 gccgacttca aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt
    30121 gatattatta aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag
    30181 ggccagcgtg ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt
    30241 ggctggtaac tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc
    30301 ggactccact cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg
    30361 tacgtcgaaa agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc
    30421 gatcaagatc ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc
    30481 ttctgtttct cacagatttt cccgatttat ccacaggatg atccagaact cgctaagtgt
    30541 aaacgatcct gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca
    30601 tagacaaaaa ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc
    30661 atcagcccgt ataatcctcc acccggcgcg ccatgctggt tttcactggt gtgaggccgt
    30721 acgttttccc tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc
    30781 ggagtgtaca attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt
    30841 ttcatttttc ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc
    30901 gccatgaaac gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt
    30961 gctcgtatgg ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc
    31021 gctcgtctga ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc
    31081 cagggagtta cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg
    31141 ggctggcacg ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat
    31201 cggtcttaca gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg
    31261 tctgacgcgt gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt
    31321 ggtggcgaaa aaaggggttg ccaacctcga taaccgtgct ctctcggaag cgttggaaaa
    31381 attatggcgc cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat
    31441 caacgcctga ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc
    31501 tacggaattg aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa
    31561 cgcgtattaa aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca
    31621 tttaatacca gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc
    31681 tgttcgtgtc tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg
    31741 cccaacagac cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac
    31801 cggccagtgg ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca
    31861 acacccgtgg tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact
    31921 ctacccagcc gttccagctg ctggaaactt caccgcagtt tatttatcag gcacagagcg
    31981 gtctgaccgg tcgtgatggc ccagataacc cggctaacgg cccgcgtccg ctgtataacg
    32041 ttgaaaaaga cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt
    32101 ataccgacgc ggcaggcaac acgtttacca aaacgtttgt cctgaaacgg ggtgattacg
    32161 ctgtcaacgt caactacaac gtgcagaacg ctggcgagaa accgctggaa atctccacct
    32221 ttggtcagtt gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact
    32281 tcgcactgca caccttccgt ggcgcggcgt actccacgcc tgacgagaag tacgagaaat
    32341 acaagttcga taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg
    32401 tggcaatgct gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca
    32461 acttctatac tgctaatctg ggtaacggca tcgccgctat cggctataaa tctcagccgg
    32521 tactggttca gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa
    32581 tccaggacaa aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt
    32641 tgtggttcat ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta
    32701 actggggctt ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga
    32761 ccaaagcgca gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa
    32821 tgcgtgagcg tctgggcgat gacaaacagc gtatcagcca ggaaatgatg gcgctgtaca
    32881 aagctgagaa ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct
    32941 tcctggcgtt gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac
    33001 tgtggatcca cgacctgtct gcacaggacc cgtactacat cctgccgatc ctgatgggcg
    33061 taacgatgtt cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga
    33121 agatcatgac ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc
    33181 tggtgctgta ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc
    33241 gtggtctgga aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt
    33301 tttcgctaaa ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc
    33361 accgcgtatc cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag
    33421 cccaggccac gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag
    33481 cccgtgaagt tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc
    33541 ttccgtttaa agacgccgat ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg
    33601 gcccgaactc gttcaccggc gaagatgtgc tggaactgca gggtcatggc ggtccggtga
    33661 ttctcgacct gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg
    33721 gtgagttttc cgaacgcgcg tttcttaacg ataaactcga cttagcccag gccgaggcga
    33781 ttgccgatct tatcgatgcc agttcggaac aggcagcccg ttcggcactt aactcgctgc
    33841 aaggcgcatt ctccgcacgg gtcaaccatc tggtggaagc cctcacccac ttgcgcattt
    33901 acgtcgaagc ggcaattgat ttccccgatg aagagatcga tttcctctcc gacggaaaaa
    33961 ttgaagccca gcttaatgac gtgattgccg atctcgatgc agtgcgtgct gaagcacgtc
    34021 agggtagttt gttgcgcgaa gggatgaagg tggtgattgc cggacgtcct aatgccggta
    34081 aatcgagcct gttaaacgcg ctggctggcc gtgaagcagc aatcgtaacc gatatcgccg
    34141 gaaccacgcg tgacgtgttg cgtgagcata tccacattga cggaatgccg ctgcatatca
    34201 tcgacaccgc cgggctacgt gaagccagcg atgaagtgga acgtattggt atcgagcgcg
    34261 cgtggcaaga aattgaacaa gccgaccgcg tgctgtttat ggtcgatggc accacgacag
    34321 acgccgttga tccggcggag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc
    34381 cgatcaccgt ggtacgcaat aaagccgata tcactggcga aacgctggga atgagtgaag
    34441 tgaacggtca tgcgttgatt cgtctctcgg caaagactgg cgaaggcgtg gacgtgctgc
    34501 gtaaccatct caaacagagc atgggctttg acaccaacat ggaaggcggc ttcctggcgc
    34561 gtcgtcgcca ccttcaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc
    34621 aactgttggg tgcctgggcg ggcgaactgc tggcggaaga gttgcgcctg gcacagcaga
    34681 acttaagcga aatcaccggg gaatttacct ctgatgattt gctggggcgg attttctcca
    34741 gcttctgtat tggtaagtaa gtcgtctaaa ttgatataaa gggcactgtt ccgttctggg
    34801 ggaaactaac tatcagaacg gaacagcgct tgttttttta tgagcatcct gcaccaaaac
    34861 tgcctttact actggctgga gcgactttca acatcttgca gtcctgtctt tacagagaac
    34921 gatattgctt tagccgatta agctcctctt gcggaaagtt aaattcgtct attgctttcc
    34981 agaatagttc tttaaataca ggattgccat ttgccttgct tattacatat ttgccttcat
    35041 tattcttcgc ttccagttgc atcatcacat ctttaagata attttcattt tcaccaacca
    35101 caattttgaa tattttcagc cagagaccca tatatatcat ccgctttctc taccgcaaga
    35161 gagaatgact tcccatcaaa gtcacgggta aactgctcca aaatctcatc gaaagtgaac
    35221 ccgtgttgca acgccaggcg aatcgccgca ttcagatata catcaatgac ctgcttactc
    35281 attgtcttat agctggcaac atacaaagag caaactgcat tatttctgct cagcgacagg
    35341 agttctctca ctacatcttt atcattttca taatggctgg ctatcacttc agctgtctcc
    35401 atccaggcag caacaatctt cgtatgccct ttcactatcg cagaatataa gcctgatgcc
    35461 ttcgtccatt tgtctgatgc tcccgccact ttttctcttg ctgccatcag tttcaatata
    35521 tcttctttat ttaattcatt tttaaacatt ttggctttta ta
//
LOCUS       DAZKIF010000036        35102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZKIF010000036 DAZKIF010000000
VERSION     DAZKIF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.036"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            102..698
                     /locus_tag="ACHOTW_001785"
     CDS             102..698
                     /locus_tag="ACHOTW_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9843905.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            785..1510
                     /locus_tag="ACHOTW_001786"
     CDS             785..1510
                     /locus_tag="ACHOTW_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP9843906.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1552..4152
                     /locus_tag="ACHOTW_001787"
     CDS             1552..4152
                     /locus_tag="ACHOTW_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFP9843907.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            4166..4714
                     /locus_tag="ACHOTW_001788"
     CDS             4166..4714
                     /locus_tag="ACHOTW_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9843908.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4729..5334
                     /locus_tag="ACHOTW_001789"
     CDS             4729..5334
                     /locus_tag="ACHOTW_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9843909.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            5355..5945
                     /locus_tag="ACHOTW_001790"
     CDS             5355..5945
                     /locus_tag="ACHOTW_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP9843910.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            5957..7066
                     /locus_tag="ACHOTW_001791"
     CDS             5957..7066
                     /locus_tag="ACHOTW_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9843911.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            7201..7995
                     /gene="panB"
                     /locus_tag="ACHOTW_001792"
     CDS             7201..7995
                     /gene="panB"
                     /locus_tag="ACHOTW_001792"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP9843912.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8007..8858
                     /gene="panC"
                     /locus_tag="ACHOTW_001793"
     CDS             8007..8858
                     /gene="panC"
                     /locus_tag="ACHOTW_001793"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP9843913.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9157..10053)
                     /gene="rpnC"
                     /locus_tag="ACHOTW_001794"
     CDS             complement(9157..10053)
                     /gene="rpnC"
                     /locus_tag="ACHOTW_001794"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFP9843914.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            10327..10707
                     /gene="panD"
                     /locus_tag="ACHOTW_001795"
     CDS             10327..10707
                     /gene="panD"
                     /locus_tag="ACHOTW_001795"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP9843915.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10711..11940)
                     /gene="yadE"
                     /locus_tag="ACHOTW_001796"
     CDS             complement(10711..11940)
                     /gene="yadE"
                     /locus_tag="ACHOTW_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9843916.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12004..12444)
                     /gene="yadI"
                     /locus_tag="ACHOTW_001797"
     CDS             complement(12004..12444)
                     /gene="yadI"
                     /locus_tag="ACHOTW_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9843917.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(12549..13319)
                     /gene="yadH"
                     /locus_tag="ACHOTW_001798"
     CDS             complement(12549..13319)
                     /gene="yadH"
                     /locus_tag="ACHOTW_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9843918.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13316..14242)
                     /gene="yadG"
                     /locus_tag="ACHOTW_001799"
     CDS             complement(13316..14242)
                     /gene="yadG"
                     /locus_tag="ACHOTW_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9843919.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14351..15013
                     /gene="can"
                     /locus_tag="ACHOTW_001800"
     CDS             14351..15013
                     /gene="can"
                     /locus_tag="ACHOTW_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP9843920.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15054..15590)
                     /gene="hpt"
                     /locus_tag="ACHOTW_001801"
     CDS             complement(15054..15590)
                     /gene="hpt"
                     /locus_tag="ACHOTW_001801"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9843921.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15796..18186
                     /gene="gcd"
                     /locus_tag="ACHOTW_001802"
     CDS             15796..18186
                     /gene="gcd"
                     /locus_tag="ACHOTW_001802"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP9843922.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18388..19938)
                     /gene="cueO"
                     /locus_tag="ACHOTW_001803"
     CDS             complement(18388..19938)
                     /gene="cueO"
                     /locus_tag="ACHOTW_001803"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP9843923.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMVGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20104..20451
                     /gene="yacC"
                     /locus_tag="ACHOTW_001804"
     CDS             20104..20451
                     /gene="yacC"
                     /locus_tag="ACHOTW_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP9843924.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20557..21423
                     /gene="speE"
                     /locus_tag="ACHOTW_001805"
     CDS             20557..21423
                     /gene="speE"
                     /locus_tag="ACHOTW_001805"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP9843925.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21439..22233
                     /gene="speD"
                     /locus_tag="ACHOTW_001806"
     CDS             21439..22233
                     /gene="speD"
                     /locus_tag="ACHOTW_001806"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP9843926.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22271..22633)
                     /gene="yacL"
                     /locus_tag="ACHOTW_001807"
     CDS             complement(22271..22633)
                     /gene="yacL"
                     /locus_tag="ACHOTW_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP9843927.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22809..25406)
                     /gene="acnB"
                     /locus_tag="ACHOTW_001808"
     CDS             complement(22809..25406)
                     /gene="acnB"
                     /locus_tag="ACHOTW_001808"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP9843928.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25761..27614
                     /gene="yacH"
                     /locus_tag="ACHOTW_001809"
     CDS             25761..27614
                     /gene="yacH"
                     /locus_tag="ACHOTW_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP9843929.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27685..29109)
                     /gene="lpdA"
                     /locus_tag="ACHOTW_001810"
     CDS             complement(27685..29109)
                     /gene="lpdA"
                     /locus_tag="ACHOTW_001810"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9843930.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29317..31209)
                     /gene="aceF"
                     /locus_tag="ACHOTW_001811"
     CDS             complement(29317..31209)
                     /gene="aceF"
                     /locus_tag="ACHOTW_001811"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9843931.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPEA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31224..33887)
                     /gene="aceE"
                     /locus_tag="ACHOTW_001812"
     CDS             complement(31224..33887)
                     /gene="aceE"
                     /locus_tag="ACHOTW_001812"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP9843932.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34048..34812)
                     /gene="pdhR"
                     /locus_tag="ACHOTW_001813"
     CDS             complement(34048..34812)
                     /gene="pdhR"
                     /locus_tag="ACHOTW_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP9843933.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8690 a         9258 c         8589 g         8565 t
ORIGIN      
        1 ttcacacccg tgataattca cccctattac atcctccaaa aactttgagt aatatccgct
       61 cgcaatatta atttcatata atttttgtaa ggatgcatgt aatgaaaaaa gcacttctcg
      121 cagccgctct ggttatggct tctggttccg ccctggctgt agatggtggt catatcgact
      181 ttaacggtat ggtacagtcc ggtacctgta aagtgggtgt ggtagatact ggtatgcata
      241 gcgttaccac tgatggcgtg gttaccctgg atactgcgaa tgttactgat acttttgctg
      301 aagttagcgc aactgctgtc ggtttactgc cgaaagagtt catgatttct gttgagtgtg
      361 atccaggtgc tccgaagaat gctgagttaa ctatgggttc tgcaagttac gcgaacacca
      421 gcggtaccct gaataacaat atgaacatca ctgttaacgg tattgcaccg gctcagaacg
      481 taaacattgc agttcataac atgaaaaaca aagctggcgc tgctgaaatt aagcaggtcc
      541 atatgaacaa ctcttctgaa gttcaggaac tgacattaga cgcagaaggt aaaggccagt
      601 acgtatttaa cgcatcttac gttaaagcac cgaacagccc ggctgtaact gctggtcatg
      661 taaccactaa cgcgctgtac accgttgctt ataagtaatt gatggtgtgt tgagcataag
      721 gtttgccagc aggcaaacct tattttcgcg aatttaatct ttctacagac agcaaggagt
      781 gcttatgttt cccttggtta aaaaaacggt tagtgcatta tttgtgagta ctctgttagc
      841 cagtgcacca gcattcgctg acattattat ttccggaaca cgtatcattt ataacgctga
      901 taaaaaagat gttaacgttc gtctggaaaa taaagggaat cgccccttac tgattcagaa
      961 ctggttggat acgggcgatg acaatgccga tcccagccag atcaaagtgc cgtttaccgc
     1021 gaccccacca gtttcacgcg tggagccaaa acgcggtcaa accgtaaaag tgatgtatac
     1081 cggtgcaaca gccctgccga aagaccgtga aagcgtctac tggtttaacg tgctggaagt
     1141 gccgcctaag ccgaaagatg ctgaggctga caaaaacttg ttacagctgg ctttccgcac
     1201 acgcatcaaa ctcttttatc gcccgagtgg gttacaggga gaaccggctg aagcaccggc
     1261 gaaaatcacc tggaaattaa acaacgctca gttacaggca aataacccaa cgccatatta
     1321 cgtgtccttt aatgaggtca agctggaaag cggcggcaaa acctacaacg tcaattccag
     1381 catggtcacg ccgttcagcc aggcaagctt tggcgtaacc agtctgccag gaagcgtgtc
     1441 atccggaaaa gtggtcttta aagcgatcaa cgactttggc ggcaacattg atggttccgc
     1501 aacattctaa aaaataaacg gattcttttt aacatcgcca ggaatgcaac cttgtaccag
     1561 tttactcatc aaaaaagccg tatcccgaaa aaaacgctac ttgcggcctg ttgtgccctg
     1621 ttttatagca gcaacggtgc tgcggcggac accgtggaat atgacagttc ctttttaatg
     1681 ggaactggcg catcaacgat tgatgttaaa cgttatgctc aaggcaaccc gacaccgccg
     1741 ggtctctata atgtccgcgt atttgtaaac ggtcaggcga cttccagctt agaaattccg
     1801 tttgtggata ttggcgaaaa cagtgcggcg gcctgtctta cccataaaaa cctggcgcaa
     1861 cttcacatta agcaacctga acagcctgtc actttactcg ccagagaagg tgaagaagag
     1921 gattgtctgg atctggcaaa gtcatacgaa aaggcggatg tgtgctttga cggtagtgac
     1981 cagtttctcg atctgacgat ccctcaggcc tatgttctga aaagctatgg cggctacgtt
     2041 gacccttctt tatgggaatc gggaattaac gctgccacac tggcatatac cctgaacgcg
     2101 tatcacacaa gttcagataa cgacaatagt gacagcgtct atggcgcgtt caactcaggt
     2161 atcaatttag gagcctggca ctttcgtgcg cgcggtaact ataactggac aacagataac
     2221 ggcagcgatt tcgatttcca ggatcgttac ttacagcgtg acattccggc aatccgttcc
     2281 cagataatta tgggtgatgc ctataccacc ggtgaaacgt ttgactctgt caacgtccgt
     2341 ggtgttcgcc tgtacagcga cagccgtatg ctgccttcgg cgctggccag ttacgctccg
     2401 accatccgcg gtgtagcaaa ctccaacgcc aaagtcaccg tgacgcaaag cggatataaa
     2461 atttatgaaa ccaccgttcc gcccggtgaa tttgttatag acgacattag cccttccggc
     2521 tttggtagcg aactggtcgt gaccattgaa gaagcggatg gttccaaacg cacctttacg
     2581 caacccttct cgtcggttgt acaaatgcaa cgtcctggtg tgggccgttg ggatttcagc
     2641 gcgggtaaag tcattgatga cagtctgcga tccgaaccca atatggggca agcctcttat
     2701 tactatggtc tgaataacct cttcacgggt tataccggca ttcagttcac cgataataac
     2761 tatcttgccg ggctgttagg tgtgggtatc aacaccagca tcggcgcctt tgcggtagac
     2821 gttacccatt cccgtgctga aattccggat gataaaacct accaggggca aagttatcgc
     2881 gtgacctgga acaaactttt ccaggatacc gggacatcat ttaacctcgc ggcgtaccgc
     2941 tattccaccc aggattacct gggcctgcat gatgcgttag tcctcattga cgacgccaag
     3001 catttgtctg ccgatgaaga caaaaacacc atgcagacgt actcacgtat gaaaaaccag
     3061 tttaccgtca gcattaacca gccattgaat atcgcctatg aagattacgg ttcgctgttt
     3121 atttccggta gctggacgta ttactgggcg gcgaacaata gccgcactga atataatgtt
     3181 ggttacagta aaagcgtttc gtggggcagt ttcagcgtca acctacaacg tagctggaat
     3241 gaagacggcg agaaagatga cgcgatgtac gtcagcgtta gcgtacctat tgagaatatt
     3301 ttaggtggca aacgtaagtc ttctggtttc cgcaatttaa atactcagct caataccgat
     3361 ttcgatggtt cacatcagtt gaatgttaac agttccggta acactgaaaa caatctggtg
     3421 aactacagtg tcaacgcagg ttatagcctc gataaaaacg ccggcgattt agcctctgtt
     3481 ggtggttatc tcaactatga atctgggtta ggcggtattt ccgcttcggc ctcggccact
     3541 tctgataaca gccaacagta ctccatctca accgatggcg gctttgtatt acacagtggt
     3601 ggtttaacgt tcactaacaa cagtttcagc agtaacgaca cgctggtgtt aatcaacgcc
     3661 ctaggtgcta aaggcgcacg aatcaataac agtaataacg aaatcgatcg ctggggatat
     3721 gccgtgacgt cctctgtcag cccatatcgt gaaaaccggg taggtctgaa cattgaaaca
     3781 ctggaaaacg atgttgaact gaaaagtacc agcgccacca ccgtaccacg tagcggctcc
     3841 gttgttttga cccgtttcga aactgacgag gggcgttctg ccgtgctgaa tattactgcc
     3901 gccaatggca aatccattcc gtttgctgcg gaggtttacc agggtgaggt gatgatcggc
     3961 agcatgggcc agggtggtca ggcatttgta cgcggtatta acgacagcgg ggaattaatc
     4021 gtgcgctggt atgaaaacaa ccaaaccatt gactgtaagt tgcactacca gttcccggcg
     4081 cagccacaaa cgcagggaag caccaacacc ttattactta acaatcttac ctgtcaggta
     4141 gcaaatcact aatatggaaa attctatgaa aaggatacta ctaacatccg cgttaatagg
     4201 cctgggttta ccggcggttg gctctgcaac agatcttaat gtcgatttta cggccaccgt
     4261 attagccacc acctgcacga taactatagt ggaagatggt ggtccagcag tgaccggtag
     4321 taacgatgaa tacagcctga caattcctga tgttggtctg gataaagtcg ctaccgctgc
     4381 gcccgaagct caggccaact ttaaattaaa ggcctccgat tgcagcaatg gttactcaaa
     4441 gatttttacc acacttacag gaacaacggt ttccggtaag ttaattgtta atgaagcaac
     4501 ctctggcgct gctggggtgg ggatggggat taagcgtaga gataccgcag actcgacttt
     4561 cttcacaccc aataatactg acaaatttga atggagcgcc gatgagaaag ccagcggtgt
     4621 gcctctgacc gtagcactgc gcgaaacaac ggcgggagca ggacgcaccg gtgcgtttca
     4681 ggcaaaagcc acctttaact tcacctacga ataacaggga cagccaccat gaaattaaaa
     4741 gtcatcgcta cactgattgc tactgttgcc gtgggtgtaa gctttaacag caattttgct
     4801 tctgcgagta caacgtccgc ttctttaacc gtaaacagta acctgactat gggtacctgc
     4861 agtgctcaga taatggataa tagtaataaa gtgatcaatg aagtggtctt tggcaatgtt
     4921 tatatttctg aactcggtgc aaaaagcaaa gtgcaacagt ttaaaattcg ctttagcaat
     4981 tgctctggcc ttccccaaaa cagcgcccaa atagtgctgg cacctaatgg tatatcctgt
     5041 gctggttctc aatcgtcatc ggcgggtttt tctaacaagt ttactgacgc tagcgcagca
     5101 accagaacgg ctgtggaagt atggactaca gatacaccgg aaagcaatgg cagtacgcaa
     5161 ttccattgtg ctcaaaagat accagtgcct gtgacgcttc ccgccgacac cacaactcag
     5221 ccttacgatt acccgttaag tgcacggatg accgttgcgg aaggtagatt ggtaaccgat
     5281 gtaagaccgg gtaatttccg ctctcccacg actttcacga tcacttatca gtaatagcgg
     5341 ataaggatat tagaatgcta tgcaggcatc ataaaatcgt acacttttta gggctggcaa
     5401 cagcccttat cacgcctttt gcctattccg gccaggatgt ggatctcacc gcaaagatcg
     5461 tgcccagcac ctgtcaggtt gaagtcagta ataatggcgt tgtcgatctc ggcacagtga
     5521 cgctggacta ttttgccgac aatgtaaccc cgacaacgga ttatgcgggt ggcaaaacgt
     5581 ttaatgtgaa tgtggtctct tgcgataaca ttcaaacgac acagtcgcaa atgaaattag
     5641 attttcagcc gcaggcggga tcgcttgcac aggcgaataa tcaaattttt agtaatgaat
     5701 atgaacagca ggctaccggg gcgaaaaacg tcggcattgt gattttttcg gcccaaccga
     5761 atcagcagac atttaacgtg cgaggaacgg atggttcctc aacagcaatt tattcggtag
     5821 cacctggcaa tgctgttccc tcaacctgga ccttttattc ccgtatgcag cgggtgaata
     5881 atgcccttcc tcctgaatcg ggaatggtga gaagccaggt tattgttaac gtatcttacg
     5941 aataataagg ataattatgc aaaggaaagg caataaactg ttgattcagt tatgcagtgt
     6001 gatactgcta ttttttacca catcctggta tgcattggcg aatgaatgtt atatagagag
     6061 aaatgctgaa ggggattatc acatgaagat aagctctact cagcttagtc tggcgtcaca
     6121 aatggtcgag gttccgacag aaatagccga agctacatgg gatgtaaata ttcaactaag
     6181 aggcgatgcc atagggtgta aatctcttgg ggatagtaag gcagttcact ttcttaatac
     6241 agctgaccca agtttaatat ccacgtacac cacaacgaat ggcgcagcgt tattaaaaac
     6301 aactgttcca ggcattgtgt attctgtcga gttattatgc cttagttgtg gtgccgcaga
     6361 tgaacttgat ttatggctac ctgcacaaag tggcgcagat aacttcatac caagcaccca
     6421 gacgaaatgg gcctatgagt acagtgatca aagttggtat ttacgttttc gcttattcat
     6481 aactcctgaa tttaaaccca agaatggtgt ttccagcgga acaacgatag caggaaagat
     6541 tgcgtcatgg tatataggta ccaatgacca gccgtggatc aacttttaca ttgacaatga
     6601 ctctttaaag tttttcgtcg atgaaccgac ctgtgcaaca gttgccctgg cacaagatca
     6661 gggcaacgtc agtggcaatc aggtaacgct tgggaacagc tatgtttcgg aagtgaaaaa
     6721 tgggcttacg cgggaaatcc ctttttctat ccgtgctgaa tactgttatg ccagtaaaat
     6781 tacggttaag ttgaaagcgg caaataaacc cagcgatgcc acactggtgg gtaaaacgac
     6841 tggctcggct tcaggcgtgg ctgtaaaagt aaattcaact tatgacaata gcaaagtatt
     6901 gttaaaagca gatggtagca acacggttga ctacaacttc gccgcctggt caaacaacct
     6961 gctgttttta ccttttacgg cgcagctggt accggatggt agcggtaatg ctgtcggtgt
     7021 tggaacattt tcaggtaacg cgaccttctc ctttacctac gaataagtaa aacgtcattc
     7081 cctcctcatc gcaccagagt tctgctctgg tgcgaacatt tccccacact aataaccatt
     7141 gcctccctgt tttcggctgt tagaatgcga tcaatctgac tttagccatc aggatacgtt
     7201 atgaaaccga ccaccattgc ttcattgcag aaatgtaagc aggaaaaaaa acgcttcgcc
     7261 accatcaccg cctacgacta cagcttcgca aaactgtttg ccgaggaagg gctgaacgtg
     7321 atgctggtgg gcgactcgct ggggatgacc gttcaggggc atgaatctac ccttcccgtt
     7381 accgttgaag atatcgccta ccacactacc gcagtacgtc gtggcgcgcc aaactgcctg
     7441 ctgttggctg acctgccgtt tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc
     7501 gcaacggtta tgcgtgccgg tgctaacatg gtcaaaattg aaggcggtga gtggctggtc
     7561 gaaaccgtaa aaatgctgac cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc
     7621 ccacagtcag tgaatatttt cggtggctac aaagttcagg ggcgcggcga tgaagcgagc
     7681 gatcgactgc ttagcgatgc attagcctta gaagccgctg gtgcacagtt gctggtgctg
     7741 gaatgcgtgc cggttgaact ggcaaaacgt attaccgaag cgctggcgat cccggttatc
     7801 ggtattggcg caggcaacgt taccgatggg cagatcctcg tgatgcacga tgcgttcggc
     7861 attaccggcg gtcatatccc taaatttgcc aaaaatttcc tcgctgaaac gggcgacatc
     7921 cgcgctgcgg tgcggcagta tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa
     7981 cacagtttcc attaaggagt gacgttgtgt taattatcga aaccctgcca ctgctgcgcc
     8041 agcaaattcg ccgcctgcgt atggaaggca agcgcgtggc gctggtacca accatgggca
     8101 acctgcatga tggccacatg aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg
     8161 tcgtcagtat tttcgttaac ccgatgcagt tcgaccgccc ggaggacctg gctcggtacc
     8221 cacgcacctt gcaggaagac tgcgaaaagc tgaacaagcg caaagtggat ctggttttcg
     8281 cgccctcggt aaaagagatc tacccgaacg gtactgaaac ccatacttac gttgacgttc
     8341 cagggctttc gaccatgctg gaaggcgcca gccggccggg gcattttcgc ggcgtgtcga
     8401 ctatcgtcag caagctgttt aacctggtcc agccggacat cgcctgcttc ggtgaaaagg
     8461 actttcagca actggcgctg atccgcaaaa tggttgctga tatgggcttt gatattgaga
     8521 ttgtcggtgt gccaattatg cgcgccaaag acggtctggc actgagttcc cgtaacggtt
     8581 atctgacggc agaacaacgc aaaattgcgc ccggtctgta caaagtttta agttcgattg
     8641 ccgacaaatt gcaggctggc gaacgggatc tcgatgaaat tattgctatt gccgggcaag
     8701 aactgaatga aaaaggcttc cgcgccgatg atattcagat tcgcgatgcc gacacattgc
     8761 tggaagtctc tgaaaccagc aaacgggcag taattctggt agccgcctgg cttggcgatg
     8821 ctcgcctgat cgacaacaaa atggtcgagc tggcgtaata cttatactct aaataattca
     8881 agttgcaagt tgcaggaagg cggcaagcga gtgacaaatt cgtcaggaac gaatttgccc
     8941 agccgcaggc tggccttcgg tgagagacat ggatgtctct cattaatccc caggagctta
     9001 cataagtaag tgactggggt tagcgaacgc agacgcagca catgcaattt gaagtatgac
     9061 gagtataact ggcgctacgg ctgatggcgc caattattaa tttgccccac gtcatggcga
     9121 tggcgtctgt aagcaattat tcataacgca gacaacttag ttaatcagcc tatcaatctc
     9181 agcaagagat aaatttgtca tctctgcaac gtcttcccga gacatccctt tactcagaaa
     9241 acgctgggcg aattcttgcc tctcctcctg tcttccctgc tgaatcccct tctcaatccc
     9301 tttctcttca aaccactgtg ccagcgtcat catagacttc cctcccgttt ccctgtctct
     9361 cagcacacca taaaacaaat ccgcttgttc agtatgaccg cgttgcagca tatagttttg
     9421 catggcaact aactgacttt cgctagtgta cccttcgtca atcagcgtga ccagttgctc
     9481 cagcaataac attaagtcgc gctggcgaat atgtttttgc agtagttcga gaatcgcaat
     9541 ccgccgatgt tgcatgattt cgtcatccgg tgtgatggtg atatccacca gcgggaaagg
     9601 actgttatag acgcgtcgcg ccagctccgg cgagtaaaac atatcaaacc agcacattga
     9661 gagcggataa ggtgtggcct cgccctgata aaagagaatc ggcactacca gcggtagttt
     9721 gtcgtgatcc gcctccagat gccgatgcat ggcggctata gaataacgca tcatgcgaaa
     9781 ggccattttc ttatccggct tgctttggtg ttcaatcaca acatgcagat aaccgggatt
     9841 gccctgcatt tgcacggaat agagcacgtc cgtgctgtgt cctttaaggc tctcttcaat
     9901 gaaactcccc gactctaaat gaagcgtgtt gaggtcacaa agttcgcgta attccactgg
     9961 caaatgtatc tccagaaagt cgcgagccgt ctccgcatgc attaaaaatt gtttaaatac
    10021 cgcgtcatgc ggtgtggtac tcggtgcatc catcttccct gtcttccgtg ttgtcttgcg
    10081 tgaaggcagt atccagtcaa ggcgtgctat cgtcagccac ctctttattg tttaccgagc
    10141 agcgttcaga gaaatatcaa ttcgttacag gcgatacatg gcacgcttcg gcgcggtacg
    10201 caaactgctg atgcaaatgc cgtcagatga gtagacacta aacaaaaatc gggcaatact
    10261 gcgtgagaat ttcctcaaag caggcaccgc ctgcttcgtt aacgacaggg tagaaaggta
    10321 gaagttatga ttcgcacgat gctgcagggc aaactccacc gcgtgaaagt gactcatgcg
    10381 gacctgcact atgaaggttc ttgcgccatt gaccaggatt ttcttgacgc agccggtatt
    10441 ctcgaaaacg aagccattga tatctggaat gtcaccaacg gcaagcgttt ctccacttat
    10501 gccatcgcgg cagaacgcgg ttcgagaatt atttctgtta acggtgcggc ggcccactgc
    10561 gccagtgtcg gcgatattgt catcatcgcc agcttcgtta ccatgccaga tgaagaagct
    10621 cgcacctggc gacccaacgt cgcctatttt gaaggcgaca atgaaatgaa acgtaccgcg
    10681 aaagcgattc cggtacaggt tgcttgattg ttatccctgc ggctggttac tcaccagccg
    10741 cgacatcgtc tccagcgaat ccgttcttaa gatataaagt cgttttagta acaacggatt
    10801 atcccccggt tttactttgc ctttcatggt tgtcaccgcc aggtgaaatc cggcatcgtt
    10861 tgctgccttc acggctttgt cattaaagcc gccaaacgga tacgaaagat accagacatg
    10921 cggattaaat tgcgccagag cgcgacgtga acgtgcaaaa tcaaacagaa tattgtgctc
    10981 actacggctc agtaatatgg gtcggcgata accatctacc cgatgcaaaa aatgggtatg
    11041 tgactggaaa tcaaatacat cgcgaatttc gttaagctca gaaacgctca taaattgcag
    11101 cgattttggt tcccacttct gcgggtgacg tttgatgcgc gaggtaacaa taaacgccgt
    11161 cgccttcatg ccatattgtt tcaacacagg atacgcatag cggctcactg acttgaggcc
    11221 atcatcaaag gtaatcacca ccgctcgcgc aggaagattg atcttattct tcacgtaacc
    11281 ttccagctgc accatgctca gtgtcgcgta tcccctgtcg cgcagccagg ccatctggtt
    11341 attgaaagcg cgtaccgagg tggtcgtcga agtatggcga aaacgggtgt tttcttcgtc
    11401 gcgcagaata tggtgatagg ttagcaccgg aaggccatta tcgggttggg catccagcgc
    11461 gctgatatag gccagtcgat cgccaatacg gatctgatac caggtttgat ttaacctgtc
    11521 tttcagttta tgcaaaatcg ggtagcgcaa attgtccgcc agtaccccaa atggcgcact
    11581 gcccacactc ggcgcgttat agaccggcgt atctttccag gtaactaagt tctgattact
    11641 cagcggcttg ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct gaaccggctc
    11701 aagatgacct ttatcgataa accctttatc aaagccaaaa ttaaatgcgt aataacttgc
    11761 ggcagtgggc tccacggcaa tgatttgtcc ggcccgaata ttccccacgg tcaccatctt
    11821 gtcaccaatt tgcgcccaga ccgcagcatt ttcgatggtt tgcatataac gggcaggtaa
    11881 cgcggcactg acacttgcgg taaacagcat cagcaggaga ataacagctt gtttgtacat
    11941 gaaaaaatca ccgagggtga aacaacgctt ttattgtagc aaaagcgcca tcagaatcgc
    12001 gggttaatac tcatacaaat tatgtttgag gacgaaaggc ggattttttt gttgctggtg
    12061 ccaaatacta ctcacctccg gcgcacccag ttcgacaata cgctcgcgaa actctgaact
    12121 ggtcatggtt tcacggcaag ccatcatctg ttcaattaac ggtaacgtga caccagaaat
    12181 cacttcgcaa cgggagtgtt tgtggcttaa taatgaagcc acgcggtacg gtggcgctcc
    12241 ggctatatcg gttaagaaga tgacaccctc accagagtcc gcttcatgca gagcatcgca
    12301 catcatgcgg ctgagcatat tagaacttaa tccgcgccag aaattcacgg cccggcactg
    12361 aagaagcgcc ccatgttttt tctccagcac atccagtatc tcttgcgccc ggtcatcgtg
    12421 acaggtaatt acccaaccta acatcgcctt cctccttagc cgaaaatcag tctaccctga
    12481 ccattgttga taaagggtga tagccgtcaa aactcctctc ccccaaatcc agaggagagg
    12541 aaatagcctt agctacgcaa accacgtcca cgttggatca gcgaccaaca gatcaaataa
    12601 aacgccacaa taaagaccac cagtacgcca aaggtagtga ccagcggaac atcattgata
    12661 ccgaggaagc cgtagcggaa accactgatc atataaacga ttgggttcag gtgcgacagc
    12721 ccttgccaga acggcggcaa caaagtcagt gagtaaaaga ccccacccaa atacgtgagt
    12781 ggcgttaaca caaaggttgg caccaggctg atgtcatcga acgttttggc aaacacaccg
    12841 ttcagcaaac ccgcaaggga gaacaacacc gccgtgagca ccagggttaa ggcaacgaat
    12901 acccacgaat gcacctgaaa cggcacaaaa aacagtgaaa ttgccgtcac cagaatgcca
    12961 acaaacagac cacgcgccac gccaccgccg acatatccgg caataatgac gtgagtcgga
    13021 accggcgcta ccagcagctc ttcaatatta cgctggaact tggcaccaaa aaatgatgac
    13081 gcgacgttgg cgtaggcgtt ggtgatcacc gacatcatga tcagccccgg tacgatgaac
    13141 tgcatgtagc tgaagccatg catatcgcca atacgcgaac caatcaggtt accgaaaata
    13201 ataaagtaaa gggtcatggt gatgactggc ggcaccagcg tctgcaccca gatacgcata
    13261 aagcgatgga tctctttcgc ccagatgctt tttagcgcca cccagtaaag atgcatcatg
    13321 cgcgatctcc ttgtttttca ttaaccagtg aaacaaacag ctcttccaga cggttagctt
    13381 tgttacgcat acttaatacc tgaatgccct gctcacttaa ctgcgtaaat acgctgttga
    13441 tcccctgctc acgcagcact tcaacttcca gcgtcgcggt atcgaccagt cgatactgat
    13501 agccatcgag cttcggtaac ggacttttcg gtgcgagatc gagaataaag gtttccgatt
    13561 tcagcttcgc cagcagcgcc ttcatcgagg tattttccac cagctcaccg tgttgaataa
    13621 tgccgatatt gcggcacagc atttctgctt cttccaggta gtgtgtggtg agaatgatgg
    13681 tggtgccttt gtcgtttaaa tccttcaaaa agccccacat tgagcggcga agttcaatat
    13741 ccacgcctgc agtcggctcg tcgagaatca gtagtttagg ttcatgcatt aacgcacggg
    13801 caatcattaa acggcgcttc atcccgccag ataacatgcg cgcacgttcg ttgcgttttc
    13861 cccataaatc gagctgctta agatattttt cgctgcggat gtacgcctct ttgcgctcca
    13921 caccgtagta ccctgcctga ttcaccacaa tttgctgcac ggtttcaaac gggttgaagt
    13981 taaattcctg cggcaccagt cccaactgac gtttagcgtt cacgacatct ttctcgagat
    14041 cgtaaccaaa tacgctgacc cgcccggagg ttttatttac cagagagctg ataataccga
    14101 tagtggtcga tttcccggcc ccgttcggcc cgagaagcgc ataaaaatca cccgcttcga
    14161 cctgcaaatc tattccacga agcgcctgaa cgccgcctgg atagtttttt ttaagctgtt
    14221 gaagttccag tgcaatggtc ataaattttt acttacctta cgttcttaca ctttatctgt
    14281 ggtttaaatc gtcccggagt tgccctatat tagccaaacg taattatttg gttacaggtc
    14341 gttaacctcc atgaaagaca tagatacact catcagcaac aatgcactat ggtcaaaaat
    14401 gctggtggaa gaggatcccg ggttttttga gaaactggca caagcgcaaa aaccgcgctt
    14461 tctatggatt ggatgttccg acagtcgcgt tcctgcagaa cgtttaaccg gtcttgagcc
    14521 gggcgaactc tttgttcacc gtaatgttgc taacctggtc attcacaccg acctgaactg
    14581 cctttccgtg gttcagtatg cagtggatgt actcgaagtt gaacacatta ttatctgtgg
    14641 ccactacggt tgcggcggcg tacaagccgc agttgaaaac ccggaactgg ggcttatcaa
    14701 caactggctg ctgcatatcc gcgatatctg gttcaaacat agctcattgc tcgacgaaat
    14761 gccgcaagag cgccgtctgg ataccttgtg tgaactgaac gtcatggaac aggtgtataa
    14821 cctgggccac tccaccatta tgcaatcagc gtggaaacgc gggcagaaag ttaccattca
    14881 cggctgggct tatggcattc acgacggctt gctgcgtgat ctggatgtta ctgccaccaa
    14941 ccgcgaaacc cttgagcaac gttaccgtca cgggatttcc aacctcaagc tgaaacacgc
    15001 caaccacaaa taaaaatgcc atgccggatg caacacatcc ggcaacttca cacttactcg
    15061 tccagcagaa tcactttgcc gatatacggc agatgacggt aacgctgtgc gtaatcaatg
    15121 ccgtaaccca ccacaaactc atccgggatc gagaaaccga taaattctac cgggacgttc
    15181 acttcacgac gggacggttt atccagcagc gtacaaatcg ccaacgactt cggttcgcgc
    15241 aggcttaaga tctcacgcac tttcgacagt gtattccccg agtcgatgat atcttcaaca
    15301 atcagcacat ccttgccacg gatatcttca tccagatctt tgaggatttt cacatcacgg
    15361 gtggtggaca tgccgctacc gtagctggag gcggtcataa agtcgacttc atgagatacc
    15421 tgaacttcac ggcacaggtc cgccataaac ataaatgagc cacgcagcag acccaccagc
    15481 accatatcgc tgccgctgtc tttgtaacgc tcagtaatct gacgacccag ttcggcgata
    15541 cgcgctttaa tctccgcttc ggggatcatt acttctacag tatgtttcat atctctaacc
    15601 atatgattta aaaacaaatc acttcgcacg ggcaggtttt ggcaccgcaa caaagcgcaa
    15661 gacacgcagt ataccagcaa aacgattcat tcgcggaatc tgttaataaa gctcaaattg
    15721 tgatgacgat cacacatgtt aaaacctata attaattgct actaaaatat taatgaattg
    15781 aaatggtgtc tctttatggc aattaacaat acaggctcgc gacgattact cgtcacgcta
    15841 acagcccttt ttgcagcgct ttgcgggctg tatctactca ttggcggagg ctggctggtc
    15901 gcgattggcg gctcctggta ctaccctatc gctggccttg tgatgctcgg cgtcgcctgg
    15961 atgctgtggc gcagtaaacg tgccgcgctt tggctatacg ccgccctgct gctcggcacc
    16021 atgatttggg gcgtctggga agttggtttc gacttctggg cgctgactcc gcgcagcgat
    16081 attctggtct tcttcggcat ctggctgatc ctgccgtttg tctggcgtcg cctggtcatt
    16141 cctgccagcg gcgcagttgc cgcactggtg gtggcactgc tgattagcgg tagtatcctg
    16201 acctgggccg gatttaacga tccgcaggaa atcaacggca ccttaagcgc cgatgccaca
    16261 cctgcggaag ctatctcacc cgttgccgat caggactggc ctgcctatgg tcgcaatcag
    16321 gaaggtcaac gcttttcacc tctgaaacaa attaacgccg ataacgtcca taatctgaaa
    16381 gaagcctggg tgttccgtac tggcgatgtg aagcagccga acgatccggg cgaaatcacc
    16441 aatgaagtga cgccgattaa agtgggcgac accctttacc tgtgtaccgc tcaccagcgc
    16501 ctgtttgcgc tcgatgccgc cagcggcaaa gagaaatggc attacgatcc tgagctgaaa
    16561 actaacgagt ctttccagca cgtaacctgc cgtggcgtct cttatcatga agccaaagca
    16621 gaaacggctt cgccggaagt gatggcggat tgcccgcgtc gtatcattct tccggtcaat
    16681 gatggtcgcc tgattgcgat taacgctgaa aacggcaagc tgtgcgaaac cttcgccaat
    16741 aaaggcgtgc tcaatctgca aagcaatatg ccagacacca aaccgggtct gtatgaaccg
    16801 acttcgccac cgattatcac cgataaaacc atcgtgatgg caggttcagt taccgataac
    16861 ttctcaaccc gcgaaacgtc tggcgtgatc cgtggttttg atgtcaacac cggggagctg
    16921 ctgtgggctt ttgatccggg cgcgaaagat ccgaacgcaa tcccgtctga cgaacacacc
    16981 tttaccttta actcgccaaa ctcgtgggca ccagcggcct atgacgcgaa gctggatctg
    17041 gtgtatctgc cgatgggcgt gaccacgccg gatatctggg gcggtaaccg cacaccggaa
    17101 caggaacgtt atgccagctc gattctggcg ctgaatgcca caaccgggaa attggcgtgg
    17161 agctaccaga ccgttcacca cgacctgtgg gatatggatc ttccggcaca gccgacgctg
    17221 gcggacatca ccgttaatgg tcagaaagtg ccagttattt acgctccggc gaaaaccggc
    17281 aacatttttg tgctcgatcg tcgtaatggc gaactggtgg ttccggcacc ggaaaaaccg
    17341 gttccccaag gtgctgccaa aggcgattac gttaccccta ctcaaccatt ctcggaactg
    17401 agcttccgtc cgacgaaaga tttgagcggt gcggatatgt ggggagccac catgtttgac
    17461 caactggtgt gccgcgtgat gttccaccag atgcgctatg aaggcatttt cacgccgcca
    17521 tctgaacagg gcacgctggt cttcccgggt aacctgggaa tgtttgaatg gggcgggatt
    17581 tccgttgatc caaatcgtga agtggcaatt gccaacccaa tggcactgcc gtttgtttcg
    17641 aaactgatcc cacgcggtcc gggtaacccg atggagcagc cgaaagatgc caaaggcacg
    17701 ggtacggaat ccggcattca gccacagtac ggtgtaccgt atggtgtcac gctcaacccg
    17761 ttcctctcac catttggtct gccatgtaaa cagccagcat ggggttatat ctcggcgctg
    17821 gatctgaaaa ctaatgaagt ggtgtggaag aaacgtattg gtacgccgca ggacagtatg
    17881 ccgttcccga tgcctgtacc ggtgccgttc aatatgggta tgccgatgct gggcgggcca
    17941 atctccacgg cgggtaacgt gctgtttatc gccgctacgg cagataacta cctgcgcgct
    18001 tacaacatga gcaacggtga aaaactgtgg cagggtcgtt taccagcggg tggtcaggcg
    18061 acgccaatga cctatgaagt gaatggtaag cagtatgtgg tgatctccgc aggcggtcac
    18121 ggttcatttg gtacgaagat gggtgactat attgtggctt atgcgctgcc ggatgatgtg
    18181 aagtaagact tgctcagatt gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga
    18241 acgccttatc cagcctacaa aattgtgcaa aatcaatgga ttgcacaggt aacgtaggcc
    18301 tgataagcgt agcgcatcag gcagatttgc gtttgtcatc agtttaatgc ccggagagat
    18361 ccgggcatat tcccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    18421 ttcatgctcc agcagatggc agtgcgccat ataagcacgt tctttcggtg cgtcgtgatt
    18481 aaacttcacc agcacttcac tgacattgcc ttcgacctta acggtatctt tccagcccgc
    18541 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    18601 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    18661 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    18721 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    18781 gcccatcatc tggctgtgat ccattccgac catcgcctga tcgccatatt tctccattag
    18841 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    18901 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtctggca aagcaccgga
    18961 agcactaata gcaatcggct gaatccgcat taccggatga ggcttatcaa acggcgcaat
    19021 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    19081 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgttca ccttcaccgg
    19141 ttcaggtagc agaccaccgt cgctggcaat cacataaagc gggcgattgt cgctggtggc
    19201 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    19261 ggcagcgtgt tgcgggtaga ttgcaccgtt ggtcagcaac gtatcgccaa accagcccac
    19321 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    19381 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    19441 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    19501 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    19561 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgactt cacccggtac
    19621 ttccagcccg tgccagtgca acgtcgtctc ttccgtcagt tggttataga tatcaaccgt
    19681 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    19741 ggtagttgca gttttctcgc caaaggtaga ctgacctgcg ccaatagtta actgaatgcg
    19801 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    19861 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    19921 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    19981 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    20041 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    20101 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatggccgt ttgcgcaaac
    20161 agttacgcgc tcagtgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    20221 tttctgaaga acgattgtgg ttaccagaac ttacctaacg ggcaaattcg tcgcgcactg
    20281 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    20341 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaag
    20401 tgcaaggccc tggctcgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    20461 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    20521 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    20581 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    20641 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    20701 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    20761 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    20821 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    20881 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctac ccaaccataa cgccggtagc
    20941 tacgacgatc cgcgctttaa gctggtgatc gacgatggcg tcaatttcgt taatcaaacc
    21001 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    21061 cttttcactt cggcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    21121 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    21181 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    21241 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    21301 attattcagg cgcgttttct cgcctctggc ctgaaatgcc gttattacaa tccggcagtc
    21361 catacggcag cttttgcctt accccagtat ctgcaagacg cactggcttc acagccgtcc
    21421 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    21481 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    21541 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    21601 acctgttcca ttatcggggc taatattctt aacatcgccc gccaggatta cgaaccacag
    21661 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    21721 acagaacacc ccggcccact gccagaaacg gtcgttgccc atctcgataa aagtcatatt
    21781 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    21841 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    21901 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac ccgcgacatt
    21961 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    22021 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    22081 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    22141 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    22201 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    22261 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    22321 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    22381 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcgccg tccatccata
    22441 gcgtatactc atgccctgcc cgctgccagg aacgttcgct acctttcagt gcgtgcgctg
    22501 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    22561 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    22621 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc accgaaatcg cattgcgcca
    22681 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgatg gtttagaaat
    22741 aaaaaaatgc ccgggaatct cattcaccgg gcattgtgtc gtttatgcgc agcgcgtgcg
    22801 ctgacttttt aaaccgcagt ctggaaaatc accccatcgg ctttctcggt gtactgagaa
    22861 agctggttga agttcagata acggtaagta tcaacggctg ttttatctac ctgcgccacg
    22921 taggtctggt actcttccgg cgtcggcagt ttgccaatca gcgccgcaac agcagccagt
    22981 tccgcagaag ccaggaagac attcgcgcca gtacccagac ggttcgggaa gttacgggta
    23041 gaggtggaaa ccaccgttgc accgtccgcc acacgcgcct ggttacccat acacagggaa
    23101 cagccaggga tctcgatacg cgcaccactc ttaccgaaga cgctgtagta gccttcttcg
    23161 gtcaactgtg cggcgtccat acgggttggc ggtgccaccc acaggcgggt cggcaactga
    23221 cctttgtgcg catccagcag tttacctgcg gcacggaagt gaccgatgtt agtcatgcaa
    23281 gaaccgataa acacttcgtc gatcttctcg ccctgtaccg cagacagcgg acgtgcgtcg
    23341 tccgggtcgt tcggagcaca gaggattggc tctttaatat ccgccagatc gatgtcgatc
    23401 actgccgcgt attccgcatc tgcatcggct tccagcagct caggattcgc cagccatttt
    23461 tccatgccct gaatacgacg ttccagggta cgacgatcgc cgtaaccttc cgcgatcatc
    23521 cacttcagca ggacgatgtt ggagttcagg tattcgatga ttggttcttt gttcagcttg
    23581 atggtacaac cggcagcaga acgctcggcg gacgcatcgg tcagctcaaa ggcctgctca
    23641 actttcagat ccggcagacc ttcaatttct aggatgcggc cagagaagat gtttttcttg
    23701 cctttcttct caacggtcag cagaccttgt ttgatcgcat acagcggaat ggcgtgtacc
    23761 agatcgcgca gggtgatgcc cggctgcatt ttgcctttga agcgcaccag aacggattcc
    23821 ggcatatcca gcggcattac gcctgttgcg gctgcaaatg ccaccagacc agaacccgcc
    23881 gggaaagaga taccgatcgg gaaacgggta tgggagtcac caccggtacc gacggtatcc
    23941 ggcagcagca tacggttcag ccaggagtgg attacgccgt cacccggacg cagcgacaca
    24001 ccgccacggt tcataatgaa gtccggcagc gtgtggtgcg tgttcacgtc aactggcttc
    24061 ggatacgccg cggtgtggca gaaagactgc atcaccaggt cagccgagaa gcccaggcac
    24121 gccaggtctt tcagttcatc acgggtcatc gggcctgtgg tgtcctgaga gcctacagaa
    24181 gtcattttcg gttcacagta cgcgcccgga cgaatgcctt tcacgccaca ggcacggcct
    24241 accattttct gcgccagcga gaagccgcga tcgctctcag cgacatcttt cgcctgacgg
    24301 aacacatcac tgtgcggcag accaagtgct tcacgcgctt tggtggtcag gccacgaccg
    24361 ataatcagcg gaatacggcc gccagcacgc acttcatcaa tcagcacgtc ggttttcagt
    24421 tcgaaggtcg ccagcagttc gccggtttcg tggttacgta cttcaccttt gtacgggtaa
    24481 acgtcaatta cgtcgcccat gttcaggtta gagacatcca cttcgattgg cagcgcaccc
    24541 gcatcttcca tcgtgttaaa gaagatgggt gcaattttac cgccgaggca caaaccaccg
    24601 ccgcgtttgt tcggcacatg tggaatatca tcgcccataa accacagaac ggagttcgtg
    24661 gcggatttac gcgatgaacc cgtacccaca acgtcaccga cgtacgccag cgggaaacct
    24721 ttctgttgca gagcttcaat ctgtttgatc gggccaacaa cacctggctg gtctggctca
    24781 ataccttcac gggcgttttt cagcatcgcc agcgcgtgca atgggatatc cgggcgtgac
    24841 cacgcatccg gtgccggaga aaggtcatcg gtgttggttt cgccagtgac tttgaagacg
    24901 gtaacggtca gtttttcagc cagcgccggg cgattcagga accattcggc atcagcccag
    24961 gactgcataa cctgcttcgc atattcgttg cctgctttcg ctttctcttc tacgtcatag
    25021 aagttatcga acatcagtag tgtgtgagaa agtgctttgg cagcgatcgg tgccagtttg
    25081 gcatcatcca gcgcatcgat cagcggatga atgttgtaac caccctgcat agtacccagc
    25141 agttcgatgg ctttttccgg agtcagcagt ggggatttgg cttcgccttt ggcgacagca
    25201 gccaggaagc ctgctttaac ataggcggct tcatcgacgc caggaggaac acggttggtt
    25261 aacagatcta acaggaattc ttcttcgccc gcgggcgggt ttttcagcag ctctacaagc
    25321 gcggccattt ggtttgcatc caggggtttg ggcgcaatcc cctcagcggc acgctcagct
    25381 acgtgcttac ggtattcttc tagcacgacg gttctcctcg ctctcattgt catagtgcgg
    25441 caggcgattc tcttcacgct cctgtgagac agcagtttgt agggtaaatg cccagtaccg
    25501 caacgggcag aatagcagga ttttgacgac gtgttaatct gtttacaaaa aagcaacata
    25561 aaaaagtggc tgaatcgtta aggatggtct agagattgtt tccctccgta aaaacttcgt
    25621 taattcgcat ggtgataatc acctttctca acgaatccca agattagtca aaatttaaac
    25681 taccgcctct ttatactcgg attcacagca cctgcgggtg gcagttcgcc gaccattgcg
    25741 atttccttga gatccgaatt atgaaaatga ctttgccgtt taaaccccat gtgctggcgc
    25801 taatttgcag tgccgggctt tgtgccgcct ctgccgggct atatataaaa agccgcacag
    25861 tggaagcgcc tgtggaaacg caatcgacac aactggctgt gtctgacgct gccgcagtta
    25921 cgcttcctgc aacggtttcc gcacctcccg taacacccgc cgtcgtcaaa tccgcattca
    25981 gcactgcaca aatagatcaa tgggtcgcgc ccgtcgcgct gtatcccgac gccctacttt
    26041 cgcaggtgct gatggcatca acctatccga caaacgttgc tcaagcagtg caatggtcgc
    26101 acgataatcc acttaaacaa ggcgatgctg ctattcaggc ggtatctgac cagccgtggg
    26161 acgccagcgt taaatcactg gtggcctttc cacaattgat ggcattgatg ggcgaaaacc
    26221 cgcaatgggt gcaaaacctg ggcgatgctt ttctggccca gccgcaggac gtgatggact
    26281 cggtacaacg attgcggcaa ctggcacaac aaaccggctc gctgaagtca tcaaccgaac
    26341 agaaagttat taccacaacg aagaaaactg taccggtaac acagacagtc acggctcccg
    26401 tcataccatc caataccgtt tcaactgcca accctgtcat tacagagcct gcaacaaccg
    26461 tcatttccat tgagcccggc aatcctgatg tggtctatat tcccaactac aacccaaccg
    26521 tggtttacgg gaactgggcc aatactgcgt atccgccggt ttatctgcca ccaccagccg
    26581 gagaaccgtt tgttgacagc tttgtacgcg gattcggcta tagcatgggc gttgctacca
    26641 cgtacgcact attcagcagc atcgactggg atgacgacga tcatgaccat catcatcatg
    26701 acgatgataa ttatcatcac cacgatggcg gtcatcgtga cggtaatggc tggcaacata
    26761 acggcgacaa catcaatatc gacgtcaaca atttcaaccg tatcaccggt gagcatctta
    26821 ctgataagaa tatggcatgg cggcacaatc caaactaccg taatggtgtg ccctatcatg
    26881 atcaggatat ggcaaagcgg tttcatcaaa ctgatgtcaa cggcggaatg agtgccacgc
    26941 agctacctgc tccaacacgc gacagccagc gtcaggcggc agcaagtcag tttcagcaac
    27001 gaacacacgc cgcccccgtc attacacgag atacccaacg tcaggcagcg gcacagcggt
    27061 ttaatgaagc tgaacactat gggagctatg acgacttccg cgacttcagc cgtcgccaac
    27121 cactgaccca gcaacaaaag gacgccgctc gtcagcgtta tcagtcagct tctcctgagc
    27181 agcgccaggc agttcacgag aaaatgcaga ctaacccgca gaaccagcag cgaagagagg
    27241 cagcgcgtga gcgcattcag cccgcctcgc ctgagcagcg ccaggcagtc cgcgagaaaa
    27301 tgcagactaa cccacagatc cagcagcgaa gagacgcagc gcgtgagcgt attcagtcag
    27361 cctcgcctga gcagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaaccaac
    27421 agcaacgtga taacgcccgc cagcgtgttc aatcagcatc acctgaacaa cgtcaggttt
    27481 ttcgggagaa agttcaggag agccgcccac aacgtctaaa cgacagtaac catactgcca
    27541 ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgagcgc ggagcaaggc
    27601 gactggaaag gtaaattaca ggcgtaaaaa aagcggcgtg gttagccgct tttttaattg
    27661 ccggatgttc cggcaaacga aaaattactt cttcttcgct ttcgggttcg gcaggtcagt
    27721 aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc
    27781 gtggatggtc agtgcgatgt cttcagcatc acaacccatt tcaattgcca ggccgatttc
    27841 acccagcagc tcgccgccgt tagtaccgac aatcgcacca ccgatcacac ggtgagattc
    27901 tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag cacgaccaga
    27961 agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc
    28021 agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg
    28081 atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc
    28141 tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt
    28201 ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg tcaacttcca cgccagcttt
    28261 gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta
    28321 acgctgcggt tcagctggtg cttttttgcc ttccatcgtc acataaatac cgtcttcttt
    28381 cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt
    28441 ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac
    28501 cacgtcaatc tgtgaaccca gcgcgtggta aacggtgccc atttccagac cgatgatacc
    28561 gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtagagtc
    28621 ccagatacgc ggatcttcat gcggaataaa cggcagttga atcgggcgag aacccgctgc
    28681 aatgatcgcg ttgtcgaagt tgatcacggt tttaccgttc tcaccttcaa cttccagggt
    28741 gttagccccg gtaaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat
    28801 accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc
    28861 gatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc
    28921 gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac
    28981 accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg
    29041 gaaggcagcg gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc
    29101 agtactcatc atgacctcta tatatttatc tccggcgggt ctgacgtttt ttttatgacg
    29161 cccattatcc accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta
    29221 aacaacaaac ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta
    29281 ccagaaaaaa gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc
    29341 agacagcgtg ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg
    29401 gtggtcgaag gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca
    29461 caccggctcc atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat
    29521 cggcgcgaag tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg
    29581 catttcgccc gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg
    29641 agacagttcg atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt
    29701 cggggtatcc accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc
    29761 ttccgacagc gaactgttga agcgaggcat ctgctcaagc gctgcagcaa cggctttcat
    29821 gatgaagaca accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg
    29881 ctgtttacgg aacgcttcca gctcggtgat atcggttttg tcgaagtgag taacatgcgg
    29941 gatcatcacc cagttacggc tcaggttcgc accagaaatt ttctggatac ggcccagttc
    30001 cacttcttcg atttcaccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg
    30061 gataccaccg ccagtcgctg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta
    30121 agcctgaacg tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgcaag
    30181 gttaacgcca aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc
    30241 attttcagca aattcagatt tgccttccgc tttcgcagcc ggtgctgctg ccggggcttc
    30301 agcttttgct gccggagccg gcgctgctgc ttcctgtttc gcaggagctg ccgcaggcgc
    30361 tgcgccttca acttcgaaga tcataatcag cgagccagtt ttcactttat cgccaacgtt
    30421 gactttcagt tccttcacga cgcctgcaaa cggagccgga acttccatag aagctttatc
    30481 gccttctacg gtaatcagtg actgttcagc ggcaactttg tcgcccactt tcaccatcac
    30541 ttcggtcact tcaacttcgt caccgccgat atccggaacg ttaacttctt tcacgccagc
    30601 cgctggtgca ggggccgctg ccggagccgc ttcctgttta gccgccggag ctgccgcgcc
    30661 tgcttcaccc gccacttcga agaccataat cagcgagccg gtagacactt tgtcgccggt
    30721 gttcactttg atttctttca cggtgcctgc aaacggagcc ggaacttcca tagaagcttt
    30781 atcgccttct acggtgatca gcgactgctc agcttcaact ttgtcgccaa ctttcaccag
    30841 gatttcggtc acttcaactt cgtcgctgcc gatatccgga acgttaacgt cttttgccgc
    30901 ggcagcctcg gggactgctg ccggagctgc ttctttcttc tcttctgcct gagcaggtgc
    30961 agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg tctgagtttt
    31021 atcgccaaca gagactttga tctctttaac gatacccgcc tgcggagacg gaacttccat
    31081 agaagctttg tcgccttcta cggtgatcag cgactgttcg gcttcaactt tgtcgcccac
    31141 tttgaccagg atctcggtga tttcaacttc atcagccccg atgtccggta ctttgatttc
    31201 gatagccatt attcttttac ctcttacgcc agacgcgggt taactttatc tgcatcgatg
    31261 ttgaatttgg cgattgcgtc agcaaccact ttcttatcga tttcgccacg tttagccagt
    31321 tcgcccagcg ccgcaaccac gacataagaa gcatcaactt cgaagtggtg acgcaggttc
    31381 tcacggctgt cggaacgacc gaagccatca gtacccagta cgcggtagtc gtcagccggt
    31441 acgtaagtac ggacctgctc agcgaacagt ttcatatagt cggtagatgc cacagccgga
    31501 gcgtcgttca tcacctgagc gatatacgga acgcgcggag tttccagcgg atgcagcatg
    31561 ttccagcgtt cacaatcctg accatcacgc gccagttcgg tgaaggaggt cacgctataa
    31621 acgtcagaac ctacgccgta atctttcgcc aggatctctg ccgcttcacg gacgtgacgc
    31681 aggatagaac cggagcccag cagctgaact ttacctttgc taccttcaat ggtttcgagt
    31741 ttgtagatac ctttacggat accttcctca gcaccttccg gcattgccgg catgtggtag
    31801 ttttcgttca gcgtggtgat gtagtagtaa acgttctctt gtttttcacc gtacatacgc
    31861 tccagaccgt catgcataat gacagcaact tcgtaagcgt aagccgggtc gtaagagata
    31921 cagttcggaa tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttca
    31981 ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga agccacgcgc ttgctggtcg
    32041 ccagccgccc agcacagatc gccgatacgc tggaagccga acatcgagta atagatgtag
    32101 aacggaatca tcggcagatt gttggtgctg taagaggtcg ccgctgccag ccaggaacaa
    32161 cctgcgccca gctcgttgat cccttcctgc agaatctgac ctttctcgtc ttctttatag
    32221 taagcaacct gctcgcggtc ctgcggggtg tactgctgac cgttcgggct gtaaatacca
    32281 atctgacgga acagaccttc cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc
    32341 agacgatctt tgatcgactt gttcttcagc atcacgttca gggcacgaac gaaagcgata
    32401 gtggtagaga tctctttgct ctgctcttcc agcagcgcgc cgaagtcttg caggcttggc
    32461 agctcaagct tctcggtgaa gttcggctga cggcttggca gataaccgtg cagtttctga
    32521 cgctgtgcgt gcagataggt atgctcttca gaaccttccg ggaaggtgat gtacggcagt
    32581 ttttcgatat ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg acgcacaccg
    32641 tccatgttca ttttcttaac ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg
    32701 ccgtaacctt taatggtatg agcaaggatt accgtcgctt tgcctttggt ttcctgcgct
    32761 ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac cacggttcag tgcccagatc
    32821 tgctcgtcag tccagtctgc aaccagtgct gcggtttcag gatatttacc gaagaagtgt
    32881 tcacgaacgt acgcaccatc tttcgatttg aaggtctggt agtcgccgtc aacggtttcg
    32941 ttcatcagct ggatcagttt accgctggta tctttacgca gcagttcatc ccaacggcta
    33001 ccccacatca ctttgatcac gttccagcca gcaccttcga agataccttc cagttcgttg
    33061 atgatcttgc cgttaccggt gaccgggccg tcaagacgct gcaggttaca gttgataacg
    33121 aagaccaggt tatccagttt ttcacgggta gcgatggtga tcgcaccttt ggattccggt
    33181 tcgtccattt caccgtcgcc gaggaacgcg taaacggtct gtttagaggt atctttcagg
    33241 ccacggtgtt ccagatattt caggaattta gcctggtaaa tagcaccaat cggacccaga
    33301 cccatagata cggtcgggaa ctgccagaat tccggcatca atttcgggtg cggataggaa
    33361 gagaggccat tgccgtgaac ttcctgacgg aagttatcca gctgctcttg agtcagacga
    33421 ccttccagga aagcacgagc gtacacgccc ggggagatgt ggccctggaa gtaaaccagg
    33481 tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac atcataaatg
    33541 gttgcggaag actggaagga cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca
    33601 cgcagaaccg tcatgatggc gttccagcgg atagctgaac gaatacggcg ttccagttcc
    33661 agattacccg gatactccgg ttgttcttca acggggatgg tgttgatgta gttgctgata
    33721 ccggtgcctg cggctacgtt gacaccgcct ttgcgggctt cagcaagcag ttggtcgatc
    33781 agatactgag cacgctcaac accttcttca cggatgaccg attcgatcgc ctggagccag
    33841 tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt ctgacatggg ttattcctta
    33901 tctatctaat aacgttgagt tttctggaac ctgtcccatt gaactctcgc cggaaagctc
    33961 aataagacag gttctacgtt tagttgccgc gcttttatat gcgcttgatt tacaacatct
    34021 tctggataat ttttaccaga aaaatcacta attctttcgt tgctccagac gacgcagaga
    34081 acgctcacgg cggctttctt cacggcttct gtcgagcaaa atttcttcga taaaggccag
    34141 atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg gccataatcg cttcaaatat
    34201 gcgggtgcgg tgactactca ccagcggcag catctcgcga cgcgaataga gcaattcgaa
    34261 gttctggcgg acattctggg ccaacatcgg ctccatacac cttagcagat gaagcagaac
    34321 cacattgtgg gccgcttcgg tgacggcaat ctgatactgg agtacggcgt ttgattccgc
    34381 gtccagatcg ccagactgct gcgccagttc tatggcgtgg tggagttcac ggatgcgttc
    34441 cttgtcttca tcggtactac gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg
    34501 tcgtgtttcg agcaagtcat actgtgattc aggatggtcg gagagcagct ccaccagcgg
    34561 atcgctgaag ctttgccata ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag
    34621 caacaagccc ttcgcttcga gacgttgaat cgcctcacgc aatgagggac gggagacgtc
    34681 aaactgtttt gccagttcgc gttccggtgg gagtttttcg cccgggcgga gagtgccttc
    34741 gaggatcaaa aactccagtt gctgctcaat cacatcggag agttttggtt ggcggatttt
    34801 gctgtaggcc atgagttcct gtcttaagcc acttgccgaa gtcaattggt cttaccaatt
    34861 tcatgtctgt gacgctaaag taacaaagta ttcaccttat gtccatacag gttttgattg
    34921 aaatcatgaa actgtgcaca ttttaacaac ttgacatata taacgtttca aagttgtaac
    34981 tatgcacaaa tgtagacttt acgtaggaaa gggatttttt aacgattatt aaactataaa
    35041 aatccgaatt gaaccgattc acttaccaat tttgtgattt ttaattcaat taaaacgaat
    35101 tt
//
LOCUS       DAZKIF010000037        34768 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZKIF010000037 DAZKIF010000000
VERSION     DAZKIF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.037"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            61..762
                     /gene="elfD"
                     /locus_tag="ACHOTW_001814"
     CDS             61..762
                     /gene="elfD"
                     /locus_tag="ACHOTW_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9843934.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            787..3386
                     /gene="elfC"
                     /locus_tag="ACHOTW_001815"
                     /pseudo
     CDS             787..3386
                     /gene="elfC"
                     /locus_tag="ACHOTW_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3377..4447
                     /gene="elfG"
                     /locus_tag="ACHOTW_001816"
                     /pseudo
     CDS             3377..4447
                     /gene="elfG"
                     /locus_tag="ACHOTW_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4475..5002
                     /gene="ycbU"
                     /locus_tag="ACHOTW_001817"
     CDS             4475..5002
                     /gene="ycbU"
                     /locus_tag="ACHOTW_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9843935.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            5010..5525
                     /gene="ycbV"
                     /locus_tag="ACHOTW_001818"
     CDS             5010..5525
                     /gene="ycbV"
                     /locus_tag="ACHOTW_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9843936.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5491..6227
                     /gene="ycbF"
                     /locus_tag="ACHOTW_001819"
                     /pseudo
     CDS             5491..6227
                     /gene="ycbF"
                     /locus_tag="ACHOTW_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6338..7348
                     /gene="pyrD"
                     /locus_tag="ACHOTW_001820"
     CDS             6338..7348
                     /gene="pyrD"
                     /locus_tag="ACHOTW_001820"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP9843937.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7522..8064
                     /gene="zapC"
                     /locus_tag="ACHOTW_001821"
     CDS             7522..8064
                     /gene="zapC"
                     /locus_tag="ACHOTW_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP9843938.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8061..9170)
                     /gene="ycbX"
                     /locus_tag="ACHOTW_001822"
     CDS             complement(8061..9170)
                     /gene="ycbX"
                     /locus_tag="ACHOTW_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP9843939.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9414..11522
                     /gene="rlmKL"
                     /locus_tag="ACHOTW_001823"
     CDS             9414..11522
                     /gene="rlmKL"
                     /locus_tag="ACHOTW_001823"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP9843940.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11534..13441
                     /gene="uup"
                     /locus_tag="ACHOTW_001824"
     CDS             11534..13441
                     /gene="uup"
                     /locus_tag="ACHOTW_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9843941.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13571..14824
                     /gene="pqiA"
                     /locus_tag="ACHOTW_001825"
     CDS             13571..14824
                     /gene="pqiA"
                     /locus_tag="ACHOTW_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP9843942.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14829..16469
                     /gene="pqiB"
                     /locus_tag="ACHOTW_001826"
     CDS             14829..16469
                     /gene="pqiB"
                     /locus_tag="ACHOTW_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP9843943.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16466..17029
                     /gene="pqiC"
                     /locus_tag="ACHOTW_001827"
     CDS             16466..17029
                     /gene="pqiC"
                     /locus_tag="ACHOTW_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP9843944.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17285..17452
                     /gene="rmf"
                     /locus_tag="ACHOTW_001828"
     CDS             17285..17452
                     /gene="rmf"
                     /locus_tag="ACHOTW_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP9843945.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17522..18040)
                     /gene="fabA"
                     /locus_tag="ACHOTW_001829"
     CDS             complement(17522..18040)
                     /gene="fabA"
                     /locus_tag="ACHOTW_001829"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP9843946.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18109..19869)
                     /gene="ycbZ"
                     /locus_tag="ACHOTW_001830"
     CDS             complement(18109..19869)
                     /gene="ycbZ"
                     /locus_tag="ACHOTW_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9843947.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20055..20507
                     /gene="matP"
                     /locus_tag="ACHOTW_001831"
     CDS             20055..20507
                     /gene="matP"
                     /locus_tag="ACHOTW_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP9843948.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20583..21623)
                     /gene="ompA"
                     /locus_tag="ACHOTW_001832"
     CDS             complement(20583..21623)
                     /gene="ompA"
                     /locus_tag="ACHOTW_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP9843949.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21980..22489)
                     /gene="sulA"
                     /locus_tag="ACHOTW_001833"
     CDS             complement(21980..22489)
                     /gene="sulA"
                     /locus_tag="ACHOTW_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP9843950.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22708..23337
                     /gene="sxy"
                     /locus_tag="ACHOTW_001834"
     CDS             22708..23337
                     /gene="sxy"
                     /locus_tag="ACHOTW_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP9843951.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23300..25462)
                     /gene="yccS"
                     /locus_tag="ACHOTW_001835"
     CDS             complement(23300..25462)
                     /gene="yccS"
                     /locus_tag="ACHOTW_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP9843952.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25472..25918)
                     /gene="yccF"
                     /locus_tag="ACHOTW_001836"
     CDS             complement(25472..25918)
                     /gene="yccF"
                     /locus_tag="ACHOTW_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9843953.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26041..28095
                     /gene="helD"
                     /locus_tag="ACHOTW_001837"
     CDS             26041..28095
                     /gene="helD"
                     /locus_tag="ACHOTW_001837"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP9843954.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28127..28585)
                     /gene="mgsA"
                     /locus_tag="ACHOTW_001838"
     CDS             complement(28127..28585)
                     /gene="mgsA"
                     /locus_tag="ACHOTW_001838"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9843955.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28681..29343)
                     /gene="yccT"
                     /locus_tag="ACHOTW_001839"
     CDS             complement(28681..29343)
                     /gene="yccT"
                     /locus_tag="ACHOTW_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP9843956.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29516..29929
                     /gene="yccU"
                     /locus_tag="ACHOTW_001840"
     CDS             29516..29929
                     /gene="yccU"
                     /locus_tag="ACHOTW_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9843957.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLDLAK"
     gene            complement(29974..30291)
                     /gene="hspQ"
                     /locus_tag="ACHOTW_001841"
     CDS             complement(29974..30291)
                     /gene="hspQ"
                     /locus_tag="ACHOTW_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP9843958.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30349..31539)
                     /gene="rlmI"
                     /locus_tag="ACHOTW_001842"
     CDS             complement(30349..31539)
                     /gene="rlmI"
                     /locus_tag="ACHOTW_001842"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP9843959.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31634..31912
                     /gene="yccX"
                     /locus_tag="ACHOTW_001843"
     CDS             31634..31912
                     /gene="yccX"
                     /locus_tag="ACHOTW_001843"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9843960.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31909..32238)
                     /gene="tusE"
                     /locus_tag="ACHOTW_001844"
     CDS             complement(31909..32238)
                     /gene="tusE"
                     /locus_tag="ACHOTW_001844"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP9843961.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32329..32988)
                     /gene="yccA"
                     /locus_tag="ACHOTW_001845"
     CDS             complement(32329..32988)
                     /gene="yccA"
                     /locus_tag="ACHOTW_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP9843962.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33396..34415)
                     /locus_tag="ACHOTW_001846"
     CDS             complement(33396..34415)
                     /locus_tag="ACHOTW_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9843963.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(34393..34635)
                     /locus_tag="ACHOTW_001847"
     CDS             complement(34393..34635)
                     /locus_tag="ACHOTW_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP9843964.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT         8929 a         8302 c         9026 g         8511 t
ORIGIN      
        1 taattagtac ccctccagtg ttatggaggg gatattcata ttttttaaga gtgactattt
       61 atgaaaactt gcataacaaa gggaattgtg accgtaagtt taacggcaat attactcagc
      121 tgttcgtcca catgggcggc aggtaaaggc ggggttggac ttgcagcaac acgtcttgtt
      181 tattcggaag gtgaagagca aatttcactg ggtgtacgta acaccagtcc ggatgttcct
      241 tatctgattc agtcatgggt gatgacccca gataataaaa aatcagcgga ctttattatt
      301 acaccaccgc tatttgtgct gaatccggca aacgagaatc tgttacgcat tatgtacatt
      361 ggcgcgccgt tggcgaaaga cagagaaact cttttcttca ctagcgtacg ggcagtccct
      421 tcaacaacga agcgggaaga aggcaatacc ctgaagattg ccacacaaag cgtcatcaaa
      481 cttttctggc gaccaaaagg tttagcgtat cccttaggcg aggctccggc gaaactgcgt
      541 tgcacttcgt cagctgacat ggttacggtc agtaacccaa caccttattt cattaccctg
      601 acagacctga aaataggtgg aaagttagtt aaaaatcaaa tgatttcccc ctttgataaa
      661 taccaattct ctctgccaaa gggtgccaaa aatagcagcg taacgtatcg aaccatcaat
      721 gactacgggg cggaaacgcc gcaactcaac tgtaaatcgt aattcgtctt cagttaagag
      781 agcgagatgt atagaactca ccgacaacac agcctgttaa gctctggtgg agtgccatcg
      841 tttattggtg ggctggtggt gtttgtgtcg gcagcgttca atgcacaagc tgaaacctgg
      901 ttcgatccag cctttttcaa agatgatccc tcaatggtgg ccgatttgtc tcgtttcgaa
      961 aaaggacaaa aaataacgcc aggggtttat cgtgtcgata ttgttctgaa tcagacaatt
     1021 gtagatacgc gcaacgtcaa ttttgttgag ataacgccag agaaggggat tgccgcctgt
     1081 ttgacgactg aaagcctgga tgcaatgggc gtgaatactg atgcatttcc ggcttttaaa
     1141 caactggaca aacaagcgtg tgcgccattg gcggagatta ttccggatgc cagcgtaact
     1201 tttaatgtga ataaactccg tctggaaatt tcagtaccgc aaattgccat caaaagtaat
     1261 gctcgtggtt atgtccccct gaacgttggg atgaagggat caacgcgcta ttactgggat
     1321 attcatttag cggggctaac agtattcata gcagcgcaga cagtgattct ggcgacagtt
     1381 attttctgaa tttaaacagt ggcgttaatt taggcccatg gagattgcgc aacaattcaa
     1441 catggagtcg tagtagtggc caaaccgcag aatggaagaa tctcagcagc tatttgcaac
     1501 gggcggttat tcctctgaaa ggcgaactga ccgtaggtga tgattatact gcaggcgatt
     1561 ttttcgatag tgtcagcttt cgtggtgtgc agctggcgtc agatgacaac atgctgccag
     1621 acagcctgaa agggtttgcg cctgtggtgc gtggtatcgc caaaagcaat gcccagataa
     1681 cgattaagca aaatggttac accatttacc aaacttatgt atcgcctggt gcttttgaaa
     1741 ttagtgatat ctattccacg tcgtcgagcg gtgatttgtt agttgaaatc aaagaagcgg
     1801 acggcagcgt caatagctac agcgtaccct tttccagcgt gccattactc cagcgtcagg
     1861 ggcgaatcaa atacgcggtg acactggcga aatacagaac caatagtaat gaacagcagg
     1921 agagcaaatt tgcccaggcc acgttgcagt ggggcggacc gtggggaacg acctggtatg
     1981 gtggtggaca atatgctgaa tattaccgtg ccgccatgtt tggtctggga tttaacctcg
     2041 gcgatttcgg agcaatttcg ttcgatgtga cccaggcgaa gagtacgctg gcagaccaaa
     2101 gcgaacataa aggtcagtca tatcgttttc tgtatgccaa aacgctcaac caattgggta
     2161 ctaactttca attgatgggc tatcgctatt cgacgtcggg tttctacacc ctttccgaca
     2221 ccatgtataa acatatggat ggctacgaat ttaatgacgg tgatgatgaa gatacgccga
     2281 tgtggtcgcg ttattacaat ttgttttaca ccaaacgtgg caaactgcag gtcaatatct
     2341 cccagcaatt aagcgagtac ggttcgtttt atttaagtgg tagccagcaa acttactggc
     2401 ataccgatca acaggatcgg ctattacagt ttggctataa cacgcaaatt aaagatctct
     2461 cgctggggat ctcctggaac tacagtaagt cccgtggcca acctgatgct gatcaggtgt
     2521 ttgcacttaa tttttccctg ccactcaatc tgttgctctc cagaagtaat gatagctata
     2581 ccagcaaaaa aaattacgcc tggatgacct ctaacaccag tatcgataac gaagggcaca
     2641 ctacacaaaa cctgggttta acggagacac tactcgatga cggtaacctg agctacagcg
     2701 tgcaacaggg atataacagc gaggggaaaa cggctaatgg cagcgccagc atggactaca
     2761 aaggggtgtt tgcagatgcc cgagtgggct acaactacag cgataacggc agtcaacaac
     2821 aactgaacta cgctctttca ggcagtttag ttgcccattc acagggcatt accctggggc
     2881 aatcactggg ggaaactaac gttctgattg cagcaccagg cgcagagaat actcgtgtgg
     2941 cgaacagcac cgggctgaaa actgactggc gcggatatac cgttgttcct tatgccactt
     3001 cttatcgtga aaatcgaatc gcacttgatg cggcgtcgtt aaaacgtaac gtggatcttg
     3061 aaaatgcagt agtcaacgtg gttcccacca aaggggcgtt ggttctggcg gagttcaatg
     3121 cccatgcggg agcaagggta ttaatgaaaa catcaaagca gggtatatcg ctgcgttttg
     3181 gcgcgatagc gacgctggac ggcgtacaga ctaatagcgg cataattgat gatgatggct
     3241 cgctctatat ggcgggttta ccggcgaagg gaacaataac cgtgcgctgg ggcgaagctc
     3301 ccgatcaaat ttgtcatatc agttacgagc ttaccgaaca acaaattaac tctgcgatta
     3361 cgcgaatgga tgccatatgc agataatctt tggagaaaaa tgcgtgtcat tactacgact
     3421 attttttgcc gccgtcttaa tgctatggtg cgctcaaacc gctgcttata gcgggcagtg
     3481 tcataccact caggggaatc cgtatattgg cgtcaatttt ggcgttaaaa ccctggagga
     3541 agaagaaaat acgactgggg tagtaaaaga caaattttat cagtggaacg aatcgaatga
     3601 ttattatgtt tcctgtgatt gcgataaaga caatgtcaga agtggccgat gggcattcgc
     3661 cgcggattca ccgttagtct atttaggcga caactggtac aaaattaatg actatcttgc
     3721 cgccaaagtt ttattgcagg ttaaaggcag ttctcctaca gcggttcctt tcgaaaacgt
     3781 ggggactggg gcagataccc ggtggcatat ttgtgacccc ggcggtcaac gtttaggcgg
     3841 ccagggagct agcggtaata gcggtagctt ttccctgaaa atattgcagc cgttcgttgg
     3901 ttcggtcgtc attcctccta tggcgctggc gcgattattt gaatgctaca acatacccgc
     3961 aggtgattcc tgcacgacta caggcacacc ggttttagtg tattacctgt ctggtactat
     4021 caattcactt ggctcatgtt ccgtcaatgc cggagaaaca atcgaggtcg atctgggcga
     4081 cgtatttgcg gctaactttc gtgttgtagg gcataagcct cttggggcca gaacggcaga
     4141 acttgcaatt ccagtcaggt gtaacacggg aaacgcgggg ttagttaacg tcaacctgag
     4201 tctgacggca accacagacc ccagctatcc ccaggcgatt aagacgtcac gtcctggcgt
     4261 gggcgtggtg gtgaccgata gccagaacaa cattatttcc cctgctggtg gaacattacc
     4321 gctctctatt cctgatgatg cagacagtat cgcgtgaatg aatgtctatc cagtcagcac
     4381 gacaggtgta ccaccagaaa ccgggcgatt tgaagccacg gcaacggtga gaataaattt
     4441 tgattaacgc gtgaacgtat gaagaaaaaa aacgatattt cagtgcgtta tcttgttctt
     4501 tagcattctt aacatccatg tcgggatggc tgggcctgaa caagttagta tgcatattta
     4561 tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac aaaatattca
     4621 tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca ctgctgctga
     4681 tttgaatatc gatatcaccg gttgtgccgc tggtattact ggcgcggacg tcctttttag
     4741 cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag gcggaagcgg
     4801 tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc aagaaatccc
     4861 gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac tcaaatatca
     4921 acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg cggttctcta
     4981 ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg attctgttct
     5041 tactgggatt aacggggggg tgtgagctat ttgcccatga tggcacggtc aacattagcg
     5101 gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc aatgtgcagt
     5161 taggcgatgt ctcactgaca cgtttttctc atgggaatta tggcccggag aaatctttca
     5221 tcattaattt gcaggattgc ggaacagatg tcagcacggt tgacgtcacc ttttcaggaa
     5281 cccccgacgg agtgcagagc gaaatgttgt ccattgaaag tggtacggat gcagccagcg
     5341 ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac caggcgagta
     5401 aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa ttgcgacctg
     5461 tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta cttcattatg
     5521 actaatacgt ggaacagatt ggcgctcttg attttcgccg ttttatcgtt gctggtggcg
     5581 ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc ggcagacaga
     5641 gaatcgatat ctattttact gactaatacc agtcaggaat cctggcttat taaccgtaaa
     5701 atcaaccgcc caacgcgttg ggccgggggt gaagcgtcga cagtgccagc accattactg
     5761 gccgctccgc cacttattct cctgaagccc ggtacgacag gcacgttgcg cttgctgaga
     5821 acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag cattgccagc
     5881 gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc ggtatttaaa
     5941 ctgttctggc gacccgaagg ttgccgggcg acccgctgga agcttatcaa caattacgct
     6001 ggacacggaa gtcgcagggt gtacaactca ctaacccaac gccttattac attaacctga
     6061 ttcaggtgag cgtaaatggt aaagcgttaa gtaatgtggg agttgtgccg cctaaaagcc
     6121 agcgtcagac aagctggtgt caggcaatag caccgtgtca tgttgcctgg cgggcgataa
     6181 atgattacgg cgggttgagt gcaaagaagg agcaaaatct gccctgaaac aggttcggaa
     6241 aacgtttgcg ttttttttgc cgcaggtcaa ttcccttttg gtccgaactc gcacataata
     6301 cgcccccggt ttgcacaccg ggaatccagg agagttcatg tactacccct tcgttcgtaa
     6361 agcccttttc cagctcgatc cagagcgcgc tcatgagttt acttttcagc aattacgccg
     6421 cattaccgga acaccgtttg aagcactggt gcggcagaaa gtgcctgcaa aacctgttaa
     6481 ctgcatgggc ctgacgttta aaaatccgct tggtctggca gccggtcttg acaaagacgg
     6541 ggagtgcatt gatgcgttag gcgcgatggg atttggatcg atagagatcg gtaccgtcac
     6601 gccacgtcca cagccaggta atgacaagcc gcgtctcttt cgtctggtag atgccgaagg
     6661 tttgatcaac cgtatgggct ttaataatct tggcgttgat aacctcgtag agaacgtaaa
     6721 aaaagcccat tatgacggcg tcctgggtat taacatcggc aaaaataaag atacgccagt
     6781 agagcagggc aaagatgact atctgatttg tatggaaaaa atctatgcct atgcgggata
     6841 tatcgccatc aatatttcat cgccaaatac cccaggatta cgcacactgc aatatggtga
     6901 agcgctggat gatctcttaa ctgcgattaa aaataaacaa aatgatttgc aagcgatgca
     6961 ccataaatat gtgccgatcg cagtgaagat cgcgccggat ctttctgaag aagaattgat
     7021 ccaggttgcc gatagtttag ttcgccataa tattgatggc gttattgcaa ccaataccac
     7081 actcgatcgt tctctggttc agggaatgaa aaattgcgat caaaccggtg gcttaagtgg
     7141 tcgtccgctt cagttaaaaa gcaccgaaat tattcgccgc ttgtcactgg aattaaacgg
     7201 tcgcttaccg atcatcggtg ttggcggcat cgactcggtt atcgctgcgc gtgaaaagat
     7261 tgctgcgggt gcctcactgg tgcaaattta ttctggtttt atttttaaag gtccgccgct
     7321 gattaaagaa atcgttaccc atatctaatt atttcttcga attcctcttt ataagataat
     7381 cagggcttta ttttcagccc tggttgtttt atattcattg ctgttgctta tttagacatt
     7441 ttgtactttt attgttgagg ttattaagcg aagcgacaat ggattgtgtt gctgcggttt
     7501 tatagggtag gggagaggca gatgcgaatt aaaccagacg ataactggcg ttggtattac
     7561 gatgaagagc acgatcgtat gatgctcgat ttagccaatg gtatgctatt tcgctcacgt
     7621 tttgcgcgca aaatgttgac tccagatgcc ttttctcccg caggcttttg cgttgacgac
     7681 gccgcgctct atttctcttt tgaagaaaag tgccgcgatt ttaatttatc taaagaacaa
     7741 aaagccgaac tggtgctgaa tgcactggta gcaattcgct atctcaaacc gcaaatgccg
     7801 aaaagctggc attttgtttc ccatggtgaa atgtgggtgc ccatgccggg cgatgcggcc
     7861 tgcgtttggt tgagcgacac taacgagcaa gtgaatttac tggtcgttga atctggcgag
     7921 aatgccgcgc tatgcctgct ggcacaaccc tgcgttgtta ttgcgggtcg cgcgatgcag
     7981 ttgggcgacg ccattaaaat catgaacgac aggctgaaac cgcaggttaa tgttgacagc
     8041 ttcagcctcg aacaggcagt ctaacgcgcc aacttaagtg cagtcttcgg tacacagcta
     8101 cagcaaagaa tggtgccatc atcgcccatt gctgattttt tcagcggcgt gacttcgcct
     8161 tctaaaagct gaacacggca acttccacaa atgcccgcgc ggcaagaata agggatacga
     8221 attccctgat tttctaattg ctccagcaac acctgttggt tatttccacg aaatgcctgt
     8281 ccctgccagt caatatctac attggcgtcc ggttgttgcg tgatgttggc agtatcatca
     8341 gcggcagctg cgccgtaaat ttttgccgga gccgttgtca gaatttccac ctcatcgcca
     8401 acgcgaatca cgccgctatt acgggcaatt aaattctgac caaaatcgac atcgccgtta
     8461 tcctgggcag tgcggaaaga ttgcaatgtt tttaatggtt cgcctgccgg atgtttttgc
     8521 cctttttctg ggctgacggt ggtgaaaata cagcggctac aaggtttaac cacatcaaac
     8581 accacatcac caatgcgaat cactttccaa ctatcttctt cccaggctga cgcgccggaa
     8641 accaccagat tggggcggaa ttgctccatt tttacactgg ccggacaacg ttgttggaga
     8701 tcacgtaacg aggcttcgtt agcaagaagg taaggatagc catcagcaaa tgacaggggt
     8761 acagtgttgt ggcgtttcac gcgccgggtc atttgtggcc ccacccagcg taattgcact
     8821 tcgcgggaga aaaatccact taaccatttg ttgatcgctt ctggcgcaat tcgcgcggta
     8881 aaatgtgtgc cccaaacttc ggttggtgcg tcttgtgtgg cgaaatcagc aaaacgaaca
     8941 tatgcgctac tgctatctgg tgcggtgaga tgcaagccat catgcacggg tgaaggagta
     9001 aatcgtacca tctggggaaa ctggcgagcg gtgataaacg taccgtcagg ttccgtgatc
     9061 ataaagatgc gatcgaaggc cagaccactg acatctgcca gagcatgtgt aagaccaatg
     9121 ccgcgcatcg atttaacagg atgaataaaa agccgggtta atgtcgccac ggtgcggtcc
     9181 tcaaatgaaa ataagccctc aactttatga catgacgcgc ttattagcta taatgcgcaa
     9241 caattttctt agcgcctgtc ccattaggct attttatttg tcagtttggc cctgggttgt
     9301 gctcgaaatc ctcacgtact tgtgtacgct ccggtttctc cgcgctgtcc atgtccagac
     9361 tgactgcaac aattacgcct actgcggtag gctcttagag taaaagtgac gatatgaatt
     9421 ctctgtttgc cagtacggcc cgtgggctgg aagagctgtt aaaaactgaa ctggaaaacc
     9481 tgggggccgt tgaatgccag gtggttcagg gtggggtcca tttcaagggc gacacacggc
     9541 ttgtttacca gagcctgatg tggagccgcc tggcctcgcg tattatgttg ccgctgggcg
     9601 agtgtaaggt ttacagcgat ttagacctct atctcggtgt tcaggcgatc aactggacag
     9661 agatgtttaa tcctggcgcg accttcgctg tccacttcag tggtttgaat gacactatcc
     9721 gcaacagtca gtacggcgcg atgaaagtga aagacgcgat cgtcgatgct ttcacgcgga
     9781 aaaatctgcc gcgtccaaat gttgatcgcg atgcgccgga tatccgcgtt aacgtctggc
     9841 tgcataaaga aaccgccagt atcgcccttg atctcagtgg tgatggttta catctgcgtg
     9901 gctatcgcga tcgtgctggt attgcgccga tcaaagaaac cctggcagcc gctattgtga
     9961 tgcgatccgg ctggcagcca ggaacaccgt tgctcgatcc gatgtgtggt tccggtacgt
    10021 tgctgattga agcggcgatg ctggcgaccg atcgcgcacc aggcttgcac cgtgggcgtt
    10081 ggggctttag cggctgggcg cagcatgatg aagctatctg gcaggaagtg aaagcggaag
    10141 cgcaaactcg cgcccgtaaa ggcctggctg agtatagctc tcatttttac ggttcggaca
    10201 gcgacgctcg ggtgattcaa cgtgcacgca ctaacgcccg tcttgcgggg attggtgaac
    10261 tgatcacctt tgaggtgaaa gatgtcgcgc aactgaccaa tccgctgccg aaagagccgt
    10321 acggcacagt gttaagcaac ccgccatacg gggaacgtct ggacagcgaa ccggcgctga
    10381 ttgcgctgca tagcctgctg ggtcggatca tgaaaaacca gtttggtggc tggaatctct
    10441 ctttgtttag tgcctcgccg gatctgctaa gctgcttgca gctgcgtgca gacaaacagt
    10501 acaaggcgaa aaacggcccg ctggactgcg tacagaaaaa ttaccacgtt gccgaaagca
    10561 ctccagacag caaaccggcg atggtagcgg aagactacgc caaccgcctg cgtaagaacc
    10621 tcaaaaaatt cgagaagtgg gctcgtcagg aagggattga atgttaccgc ctgtatgacg
    10681 ccgatctgcc ggaatataac gttgccgttg accgttatgc cgactgggtg gtggtgcagg
    10741 agtatgcgcc gccaaaaact attgatgcgc acaaagcgcg tcagcgtctg ttcgatatta
    10801 tcgctgcaac catttcggta ctggggattg cgccaaacaa actggtgctg aaaacccgtg
    10861 aacgtcagaa gggcaaaaat cagtaccaga aactgggcga gaagggcgag tttctggaag
    10921 ttaccgaata taacgctcac ttgtgggtga acctgacgga ttatctcgat actggtctgt
    10981 tcctcgatca ccgtatcgcc cgtcgtatgc tcggccagat gagcaaaggc aaagatttcc
    11041 tcaacctgtt ctcttatacc ggcagcgcca ccgtgcatgc gggattaggc ggtgcacgca
    11101 gcaccaccac cgtggatatg tcgcgtactt atctggagtg ggcagaacgc aacctgcgtc
    11161 tgaatggcct aaccgggcgt gcgcatcgcc tgattcaggc cgattgcctg gcgtggctgc
    11221 gtgaggcaaa tgaacagttc gatctgatct ttatcgatcc gccaaccttc tctaactcaa
    11281 aacgaatgga agatgcgttt gatgttcagc gcgatcatct ggcgctgatg aaagatttga
    11341 aacgtctgct gcgtgcaggt gggacgatca tgttctcgaa caacaaacgt ggcttccgta
    11401 tggatctcga cggcctggca aaactgggac tgaaagcaca agaaattacg caaaaaacgc
    11461 tctcccagga tttcgcccgt aaccgccaga tccacaactg ctggctgatt accgcagcct
    11521 gaaaggaata gtaatgtcat taatcagtat gcatggcgca tggctgtcgt tcagcgacgc
    11581 gccgcttctc gataacgcag aactgcatat cgaagataac gaacgtgttt gtctggtggg
    11641 ccgcaacggc gcaggcaaat cgacgttaat gaaaatcctc aaccgtgaac aagggctgga
    11701 tgacggtcgc attatttacg agcaggattt gattgtagcg cgtctgcaac aggatccgcc
    11761 gcgtaacgtt gagggtagtg tttatgattt cgttgccgaa ggcattgaag aacaagcgga
    11821 atacctgaaa cgctatcacg atatttcgcg cctggtgatg aacgacccga gcgagaaaaa
    11881 tctcaacgaa ctggcgaagg ttcaggaaca gctggatcac cacaacctgt ggcagctgga
    11941 aaaccgcatc aacgaagtgc tggcgcaact ggggttagat cctaacgttg cgctgtcgtc
    12001 gctttccggc ggctggttgc gtaaagcggc attaggacgc gcgctggtga gcaatccgcg
    12061 cgtattgctg ctggacgaac cgactaacca cctggatatc gaaaccatcg actggctgga
    12121 agggttcctc aaaaccttca atgggacgat tattttcatc tcccacgacc gttcgtttat
    12181 ccgcaatatg gcgacgcgca ttgttgatct cgatcgcggc aagctggtga cctatccggg
    12241 caattacgat cagtacctgc tggaaaaaga agaagccctg cgcgtggaag aactacaaaa
    12301 tgccgagttt gatcgcaaac tggcgcagga agaagtgtgg attcgccagg ggatcaaagc
    12361 acgccgtacc cgtaatgaag gccgcgtacg cgccctgaaa gcgatgcgtc gcgagcgtgg
    12421 cgaacgtcgc gaagtgatgg gtaccgcaaa gatgcaggtg gaagaggcca gccgctccgg
    12481 taagatcgtt ttcgaaatgg aagacgtttg ctaccaggtt gacggaaagc aactggtgaa
    12541 agatttttct gcccaggttc tacgtggcga caaaattgcc ctgattgggc cgaatggctg
    12601 cggcaaaacc acgctgctga aactgatgct cggtcagctt caagcggaca gcgggcgtat
    12661 tcacgttggc accaaactgg aagtggctta tttcgatcag caccgcgcgg aactggatcc
    12721 cgataaaacg gtgatggata accttgccga aggtaagcaa gaggtgatgg ttaacggcaa
    12781 gccacgccac gttttgggct atttgcagga cttcctgttc catccgaaac gggcgatgac
    12841 gccagtacgt gcgctttctg gcggtgagcg gaaccgcttg ctgctggcgc gtttgttcct
    12901 caaaccaagc aacttattga ttcttgacga accgaccaac gatcttgatg tcgaaacgct
    12961 ggaactgctg gaagaactga tcgacagcta ccagggcacg gtattgctgg taagccacga
    13021 tcgtcagttt gtcgataaca ccgttacaga atgctggatc ttcgaaggcg gcggtaaaat
    13081 tggtcgttat gtcggcggtt atcatgatgc ccgtggtcag caagagcagt atgtggcgct
    13141 caaacagcct gcggtgaaaa aaaacgaaga acccgccgcg ccaaaagcag aaactgtaaa
    13201 acgcagcagt agcaaactaa gctataaatt gcagcgcgaa ctggagcagc taccgcaatt
    13261 gctcgaagat ctggaggcga agctggaagc cctacagacg caggtggcgg atgcttcctt
    13321 cttcagtcag ccgcatgagc agacacaaaa agtgctcgcc gatatggctg ctgcagagca
    13381 ggagctggag caagcctttg aacgctggga gtatcttgaa gcgttaaaaa atggtggctg
    13441 atcgcaaaat agtcgattta aacagagaca gctgacaacg gtaaaattgc ctgatgaact
    13501 gggtttatca ggcatgaaaa caaacacttt gttagtcatc tgtaaaatag cgcatcatta
    13561 aggagtacca atgtgcgaac atcatcatgc cgcgaagcac atcctgtgct cgcagtgtga
    13621 catgctggtg gcgttaccgc gccttgagca tggtcagaaa gcggcatgtc cccggtgtgg
    13681 cacaacgtta accgtggcgt gggatgcccc ccggcagcgt ccgaccgcct atgcgttggc
    13741 tgcactgttc atgctgttgc tgtccaactt gtttcctttt gtgaatatga acgttgcagg
    13801 agttaccagt gaaattacat tactggaaat tcccggcgtg cttttttctg aggactacgc
    13861 cagcctcggc acctttttcc tgttgtttgt gcaactggtt cccgcgtttt gtctgataac
    13921 cattctgtta ctggtgaatc gcgcggaatt accggtccgt ttaaaagagc aactggcacg
    13981 ggtgcttttt caactcaaaa cctggggaat ggcggagatt ttcctcgcgg gtgtgctggt
    14041 cagtttcgtt aaactgatgg cttacggcag cattggcgta ggcagcagct ttctcccctg
    14101 gtgtttattt tgtgtcctgc aactgcgcgc ttttcagtgc gttgatcgtc gctggttatg
    14161 ggacgatatc gccccgatgc cagaactgcg ccagccgcta aaaccaggcg tcacggggat
    14221 acgtcagggg ctgcgttcgt gctcctgttg tacggcaatc cttcctgctg atgaacccgt
    14281 gtgcccgcgc tgtggtacta aagggtacgt tcgacgtaga aacagcctgc aatggacact
    14341 cgcgctgctt gttacgtcca tcatgctgta tcttccggct aatattttgc ccatcatggt
    14401 gacggattta ttaggctcga agatgccatc gacgattctc gctggggtca tcctgttatg
    14461 gagcgaaggc tcttatcccg tcgctgcggt gatctttctg gccagtatta tggtgccaac
    14521 gttaaagatg atcgccatcg cgtggctgtg ttgggatgcc aaagggcatg gcaagcgcga
    14581 cagtgaaaga atgcatttga tttatgaagt tgttgagttt gtaggccgct ggtcgatgat
    14641 tgacgttttc gttatcgcgg tgctctcggc gctggtgcgt atgggaggtt taatgagtat
    14701 ttatccggca atgggtgcat taatgtttgc tttagtcgtc ataatgacaa tgttttctgc
    14761 tatgacgttt gacccgcgtt tgtcgtggga tcgtcaacct gaatcagagc atgaggagtc
    14821 ctgacagtat ggaatctaat aatggggaag ccaaaatcca gaaagtgaag aactggtctc
    14881 ccgtgtggat atttcctatc gtcacggcgc tcattggggc ctgggttctt ttttatcatt
    14941 acagccatca gggaccggaa gtgaccctga tcaccgcgaa tgcggaagga attgaaggtg
    15001 gcaaaaccac cattaaaagc cgtagcgttg acgtcggcgt ggttgaaagc gccacactgg
    15061 ctgatgattt gacacacgtt gaaatcaaag cgcggctgaa ttccggtatg gaaaaattgc
    15121 tgcataaaga taccgtcttt tgggtggtga aaccgcagat tggtcgagaa gggattagcg
    15181 gtctgggaac cctgctgtct ggggtttata tcgaactgca gccaggcgcg aaaggcagca
    15241 aaatggataa atacgatttg ctggactcgc caccgttggc cccgcctgat gcgaaaggta
    15301 tccgtgtggt tctcgatagc aaaaaagccg ggcagctctc gccaggagat ccggtgctgt
    15361 tccgtggcta tcgggtaggt tcggttgaaa ccagcacctt cgatacgcaa aaacgcaata
    15421 tcagttatca actgttcatc aatgcacctt atgaccgact ggtgaccagc aatgttcgct
    15481 tctggaaaga tagtggcatt gcggttgatc tgacgtcagc gggaatgcgt gtggagatgg
    15541 gctcattgac aacgctgctg agtggcggtg tcagctttga tgtgccggaa ggtctggatt
    15601 tagggcagcc agtggcaccg aaaacagctt tcgttttgta tgatgatcag aagagcattc
    15661 aggattcgtt gtacaccgat cacattgatt atctgatgtt ctttaaagat tcggtacgcg
    15721 gtctgcaacc gggagctccg gtagaattcc ggggtattcg cctcggtacc gtaagcaaag
    15781 tgccattctt tgcgccgaat atgcgtcaga catttaacga tgattaccgt attccggtgc
    15841 tgattcgtat cgagccagag cggctgaaaa tgcagcttgg cgaaaatgcg gatgttgttg
    15901 agcaccttgg cgaattgttg aaacgtggtt tacgcggatc gctgaaaacc ggaaacctgg
    15961 tcactggcgc actgtatgtt gatctcgatt tctatccaaa tacgcctgca ataaccggta
    16021 ttcgtgaatt taatggttat cagattatcc cgaccgttag cggcggcctg gcgcaaatcc
    16081 agcaacgact gatggaagcg ttggataaga tcaacaaact gccattgaat ccgatgattg
    16141 aacaggcaac cagtacgctt tctgaaagtc agcgcacaat gaaaaacctg caaacgacgc
    16201 tggatagcat gaacaagatc ctcgccagcc agtcgatgca gcagttgccg acggatatgc
    16261 agtcaacgtt gcgtgaattg aatcgcagca tgcagggctt ccagcccggc tccgcagcct
    16321 acaacaagat ggtggcggat atgcagcgcc ttgatcaggt gttgcgagaa ctgcaaccgg
    16381 tgctgaaaac gctcaatgag aagagtaacg cgctggtatt tgaagcgaag gacaaaaaag
    16441 atccagagcc gaagagggcg aaacaatgaa aaagtggcta gtgacgattg cagcactgtg
    16501 gctggccgga tgcagctccg gcgaaattaa taaaaactat taccagttac ctgtggtgca
    16561 gagcggtaca caaagtaccg ccagccaggg caatcgcctg ttatgggtag agcaggtcgc
    16621 tgtacctgac tatctggcgg ggaatggtgt ggtttatcaa accagtgatg tgaagtatgt
    16681 gattgccaac aacaacttgt gggccagccc gttggatcaa cagttgcgca acaccctggt
    16741 agccaacctg agcacgcaac tgcccggctg ggtggttgcc tcccagcctc tgggaagcgc
    16801 ccaggacacg ctcaatgtta ccgtaacgga gtttaacggt cgctatgatg gcaaggtcat
    16861 tgtcagtggt gagtggctgt tgaaccacca gggacaactg atcaaacgtc cgttccgtct
    16921 ggaaggagtg caaactcagg atggttacga tgagatggtt aaagtgctgg ccggtgtctg
    16981 gagtcaggaa gccgcttcta ttgcacaaga gataaagcgt ctaccttaat tataaagatt
    17041 tgtaaatata accgtctccg gtatgttgcc tgaggcggtt tttttgtttc taacgtgcgg
    17101 aaaaatttgt tcctcttcac attttttgta caaccgacat gcccgtgtag ctcacaaata
    17161 tgacagtggc gtgaattttg cgcattgacg gcagttatga ttcgcggtat tgcttaactg
    17221 tgattgcaca tttagtaatc actgttttct tttccaccag aaaccagtat gagggaaacg
    17281 aggcatgaag agacaaaaac gagatcgcct ggaacgggca catcaacgtg gttatcaggc
    17341 cggcatcgcc ggacgctcaa aagaaatgtg tccctatcag acgctgaatc aaaggtcaca
    17401 atggctggga ggctggcgag aagccatggc ggacagggta gtaatggcct gattctgtct
    17461 ctttaaaaag aaacctccgc attgcggagg tttcgccttt tgatactctg tctgattata
    17521 atcagaaggc agacgtatcc tggaacagac cgactttcag gtcgctggcg gtatagatca
    17581 gacgaccatc aaccagcact tcgccatccg ccaggcccat aatcagacga cggttaacaa
    17641 tgcgtttaaa gtgaatacgg taggtcactt ttttcgctgt cggcagtacc tgaccagtga
    17701 atttcacttc gccaacgccc agcgcgcggc ctttaccttc gccgcccagc cagccgaggt
    17761 agaaccctac cagctgccac attgcgtcca ggcccaggca tcccggcata accggatcgc
    17821 caataaagtg gcatccgaag aaccacagat ccggattgat atccagttct gcttcaacat
    17881 accctttgtc gaagttacca cccgtttcgg tcattttgac cacacggtcc atcatcagca
    17941 tgttcggtgc tggcaattgc gggcctttag cgccaaacag ttcaccgcga ccagaggcaa
    18001 gaaggtcttc ttttgtatag gattcgcgtt tatctaccat gttctctgta agccttattt
    18061 tattgaagca cgcaggatag ctaacacgtg tacgctgaac aagtccgatc agttcggaat
    18121 aaaccagttc agccaacgta atggccatgg aaaacggtga cgtccttcct gttgcgatgc
    18181 ttgcgcgata cgttcctgga tggtttgcat cagcgtcgtt tggccttcgc catcccacac
    18241 cagatttaat aataacggca gtgcgtcagt cacatcgtct actgcccaga tggtgaattt
    18301 gccttcttct accgctttca ccagttcact gtgaagactt aaatggcgaa cgttagctgt
    18361 ggggataatg acaccttgtt tcccggttaa ctcacgttgc tggcaaatag caaagaagcc
    18421 ttcgattttc tcatttaaac caccgaccgg ctgggcgcga ccgaactgat cgactgaacc
    18481 tgtgatagcg atactctgat tcaccggcac atcggcgagg gcgcttatca gggcgcagag
    18541 ttcagccatc gaggcactat ctccatcaac ttcactgtat gactgctcaa atgtcagcga
    18601 tgctgagaag gggatctgtt gctcaagctg tagttccgac atcaggaacg cttgcatgat
    18661 catcatccct ttcgcatgga tattgccgcc aagctccgct ttgcgttcga tgtcggtgaa
    18721 ttcaccatcg ccaatatgca caacgcagct aatgcgagaa ggttcgccaa aagcgcgtgg
    18781 atgacccgga aattcaatga ccgaaagggc gttaatttgc ccgatgcgtt cgccttcggt
    18841 ttcaatcagg atttgctcct gaaggatctc atcctgcata cgttcagcga ggaaaccttc
    18901 gcgccattca cgctgctgca gcattaagtt tagctgctcg ccggagaagg tgtcgccatc
    18961 acacagggag gcgacctctt tacactggcg gaggatccac tgcgggctaa gcggaagtgt
    19021 ttcttgttca ccggtgtagc gtgctgcttc gcggataagt accggccagg catccgctcc
    19081 cggggcaggt aagtgattat gtctggcggt aaatgtcacc cagcgacacc actgggttac
    19141 tgactccgca tcgacaatct gcagagtatc ttcaaattcg ctataaatag cctgctctga
    19201 aagctctggc tccatctcct ggaaatcagc caatgattcg cgttcgccta ccagaatgac
    19261 tttcagcttc aatggcatcg aaggcacaga gacggggaga gggcgcgact catcaaacgc
    19321 aacccagtca aaacgctcgc ggttaacgat atttttcagc cgcatccaca gcagaggttg
    19381 cgccagcagt gtacgcaaag agatgatgag aataccgcca tttgcctgat gcaccagacc
    19441 aggctgcagg gtaatgtcgc cattaaactg gcgcaggcag ccaaagagtt gctccgcttc
    19501 tacccagtcg gcagcgacaa cttgcgttaa agtcgcaaaa ttatcatctg cactcactgc
    19561 gtgacgtaag cggatggagt ggccggaaac ctcatagtga ccgcccacca gttggcctgc
    19621 atcgctttgt agcgtacgcg cggcattggc aataagattc agatactcag actcttccgg
    19681 ggccttcgcc agcataaaag aggaggatgc tcgcgtatgc agcaactgct ccagcgcaaa
    19741 ttgcagccgc ggttgagtat cactgaataa aggatcgttt tcgtcaatca aatgtggctg
    19801 agcaaatatt tcctgatagc tatcggtatc aggaaccagg tcacgccatg caagtttcgt
    19861 aatggtcaaa gttgatgttt tttagtctgt tgtcaaagcc gcgattatac cgtaaccggc
    19921 actacagcac acgtagaaag caccgacaat actcctggca tgggcgttaa agctcacagg
    19981 atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag ttacacggta
    20041 acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt tggaaatgga
    20101 agtatctggt gaagaagcat cgcgaagggg agttaatcac ccgttacata gaagccagtg
    20161 ccgcccagga agccgtcgat gtgttgctct cgctggaaaa tgaacccgtg ctggtaaatg
    20221 gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcaa accattcggg
    20281 caagacgaaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa tcgatcgatc
    20341 tggaatttat tgtctggcaa cgtctggctg gtcttgcgca gcgtcgcggt aaaacgcttt
    20401 ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac gcgaataaaa
    20461 tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg acgaaagtca
    20521 gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta ccagacgaga
    20581 acttaagctt gcggctgagt tacaacgtct ttgatacctt taacttcgat ctctacgcga
    20641 cgatccggag ccaggcagtc gatcagtgca gcacgctgtt tcacgttgtc acaggtgttg
    20701 ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc cgggatacct
    20761 ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc ctggttgtaa
    20821 gcgtcagaac cgatgcggtc ggtgtaaccc agaacaacta cggaaccgtc tttcggatcc
    20881 aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg tttcagggtt
    20941 gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt ctgtacttcc
    21001 ggtgccggag ctggagccgg agcaactact ggagctgctt cgccctgacc gaaacggtag
    21061 gaaacaccca ggctcagcat gccgttgtcc ggacgagtgc cgatggtgtg tgcgtcaccg
    21121 atgttgttgg tccactggta ttccagacgg gtagcgattt caggagtgat cgcgtactca
    21181 acaccgccag cgaagaccgg agaaacgccg gtgtcgtggt ttttaccata aacgttggat
    21241 ttagtgtctg cacgccatac cataccaccc agacgagtgt agatgtccag gtcgtcagtg
    21301 attgggtaac ccagtttagc ggtcagttga acgccctgag ctttgtatgc accgttttca
    21361 acgctgcctt tgtacggcat acgacctaac cagtcgtaac ccatttcaaa gccaacatac
    21421 gggttaacct ggtaaccacc aaaagcacca gcgcccagtt ggttttcatg ggtcgggcca
    21481 ttgttgttga tgaaaccagt gtcatggtac tgggaccagc ccagtttagc accagtgtac
    21541 caggtgttat ctttcggagc ggcctgcgct acggtagcga aaccagccag tgccactgca
    21601 atcgcgatag ctgtcttttt cattttttgc gcctcgttat catccaaaat acgccatgaa
    21661 tatctccaac gagataacac ggttaaatcc ttcaccgggg gatctgctca atattaactc
    21721 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    21781 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    21841 ttaatctttt ttgtcagcga caatttacag aagagaatcg cggaaaccgc ttcagacaag
    21901 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    21961 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22021 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22081 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22141 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22201 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22261 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22321 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22381 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    22441 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    22501 cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    22561 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    22621 cgcttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    22681 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    22741 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    22801 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    22861 gcttgtgagc aaagtgcaca gtactgtgta aaatatccgc ctgtctggct gacatataaa
    22921 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    22981 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23041 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23101 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23161 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23221 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23281 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23341 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    23401 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    23461 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    23521 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    23581 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    23641 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    23701 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    23761 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    23821 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    23881 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    23941 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24001 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24061 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24121 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24181 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24241 agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24301 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24361 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    24421 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    24481 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    24541 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    24601 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    24661 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    24721 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    24781 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    24841 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    24901 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    24961 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25021 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25081 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25141 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25201 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25261 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25321 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25381 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    25441 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    25501 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    25561 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    25621 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    25681 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    25741 cgacatgaat agcttcattg ccataaggca ccagagacag tttagtgatc tcccagcagg
    25801 atcgtgtcag cggtaaggta aaaatcagcg caatactgac cagagtcgcc aacagccagc
    25861 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    25921 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    25981 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26041 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26101 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26161 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26221 ctgccggacg aaaaagtggt gcgtctgcac ggtaccgaat ggggcgagac gcagcgtttt
    26281 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26341 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    26401 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    26461 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    26521 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    26581 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    26641 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    26701 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    26761 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgt
    26821 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    26881 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    26941 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27001 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27061 aacttctggg atgatgaaaa attacagcgt cgtcttgcct cacgcctcga tcgttgggta
    27121 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27181 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27241 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27301 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27361 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    27421 acgttgttcg ccgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    27481 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgctgtga tttagacacg
    27541 acttaccgtt ttaacagtcg tatcggtgag gtggcaaacc ggtttattca gcagaaccca
    27601 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    27661 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    27721 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    27781 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    27841 caggcggatt acgtcctcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    27901 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    27961 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gtcatcgggt atgggcactg
    28021 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28081 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28141 ccgcgagata acgctgataa tcggggatca gaatatcgac cgcgtcgttg aaatgcggcg
    28201 actggataat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28261 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28321 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28381 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    28441 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    28501 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    28561 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    28621 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    28681 tcaggaagat ggttgttttt ccgcccattg caggaaagta tttcgcgttt gtgggtcagc
    28741 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    28801 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    28861 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    28921 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    28981 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29041 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29101 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29161 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29221 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29281 ggtggcaaaa acggataccg gcaaacataa tgcaatcaag gtggtcacga tgccggtttt
    29341 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    29401 catacttact aacatataga catatttccc gcttttgctc tcattcattc gtattagctg
    29461 catggttggc atgtcggctt tgtcgtacac tttgtgaaac agtctggagg aaataatgaa
    29521 agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg tgggggcaag
    29581 cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca
    29641 cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtacgg
    29701 cacgctggca gacgttccgg aaaaagtcga tatggtcgat gttttccgta attcagaagc
    29761 ggcgtggggc gtagcgcagg aagctatcgc cattggcgcg aaaaccctct ggatgcaact
    29821 gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga atgtggtgat
    29881 ggatcgctgc ccggctattg agatccctcg cctggacctg gccaaataaa aaatccccgg
    29941 aaggcaaaaa ccttccgggg atctgttcag ggattagtta cgcagacgcg gggcctggag
    30001 ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg
    30061 caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc
    30121 ctccatcacc acgtgatacc acggagcggc gcgaagctcg tcattcaccg ccagctcatc
    30181 aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc
    30241 taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca tagtcacctc
    30301 ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt acatgacgcg
    30361 acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg gatgatcggc
    30421 cgcctgacgg aactgctcta taaattgtac atcacgaccg gcatcaattg cggcatccgc
    30481 gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat
    30541 accgccttca ttcagcagct gaatcgccag catgttgata tctttatagc cacgacacgc
    30601 gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa
    30661 tttttcaccg cgatcgcgat aggtacgcag caatttaaag acatcatcac ggacaaactc
    30721 agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagtgcttc
    30781 ctgggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa
    30841 accaccggta taggagaaac agttcagtac gcgtttattt tcaacgtagc ggcgggtagc
    30901 caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gtatgtccac
    30961 cagcagcttc atgccatgtt cttcaatcgg cagcagggca ggcggcaact caccggtgac
    31021 gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta
    31081 aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatattc
    31141 tgcgccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc
    31201 cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc
    31261 cagccagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat
    31321 agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc
    31381 gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc
    31441 tttaccttcc atacgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga
    31501 tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc
    31561 aaagaaatgg gcatcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat
    31621 aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg
    31681 cgtaggattt cgctacacca cacagtacga agcgaaaaga ctgggcttaa ccgggtacgc
    31741 caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtaga
    31801 aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct
    31861 cagcgagccg catcatccct cgggggaatt aaccgatttt cgtatccgtt aaatacattt
    31921 taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag
    31981 tcggtataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac
    32041 cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac
    32101 cacttcccag tgttctggcg acagcgaaat cccttcgttc tctgcaatca ccactgccag
    32161 cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt
    32221 accttcgaag atcagcataa ttattactac cttaatcaga ctgccggcag tgtaacaaaa
    32281 aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct
    32341 ggcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct
    32401 aaccgtggca cgaatatagt tcgtttcacc gccatgaatg atgttgctgg tttcaaacaa
    32461 aatagcgcca gaggagatca aaatgaagac cgcgctgatc gccagatgca gagcaggcag
    32521 ctgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg ccatcagcat
    32581 accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa
    32641 cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata
    32701 ggtattcaga attggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat
    32761 aatcccggtc ggcttattcg ccattttata ggtcaggaac atcaaaccat acatacccac
    32821 cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat
    32881 cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca ctttatgggt
    32941 gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc
    33001 tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac ccagtagttt
    33061 tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag
    33121 caaatataac gccctgagaa tttcggcagg caaaagaaaa aggggttagc atttagctaa
    33181 ccccttatct tatctggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
    33241 ggttttcaag accgaagaaa aacaaataaa aatcagtgta atacgtgaaa ttgctgaaaa
    33301 gttaatcaaa tttaacacca agaaaaatca aggtattagg aagttgctca tagctagtat
    33361 accaagtctt tttgccagaa atttttctgg attgtctact tgctaaccac gagcggcaaa
    33421 tccagtgttg gtgtgatttt tgttttacga tcgtaaatca acacctgatt ttctgtttta
    33481 tgcccgctga aaatttgttt gtcgcgactg ctgccttcgt aatctgagat ccccttggct
    33541 tttatgtcgt gaaaattgca cccgaacgga acgccaactt tttgctcggc tgcgcgttta
    33601 gcctgattcc accagttatt cagcgtttta gctatgactt tcccgccttt ggttgtattg
    33661 atcacatatt cgcatgtaca ggaagataca tttcgggcta actggatcgc tgtacgtaat
    33721 cgcggtgacc attccttgat ttgtttggtg ccggttttgt tttgctcaat gtagatccct
    33781 ttatccataa tatcctgcca tttcaactca agcacatcac cgagtcttgc cgcacagaga
    33841 taggaaatct ccattgcaat gcgtaactgt ggaattgctt ccgcatatat cgccgcatac
    33901 tgttcatcgg tgatgtaaac agtgcgggct ttaagagaga attttctgac tcctttgcat
    33961 gggttattct tcacataccc acgctcatat ccccacccgt atactcgact caggcttgcc
    34021 agttcatgat ttgcctgggt cttgctctca agccctcgtt tatccatgaa aattcttacc
    34081 tgctcagttt tgacattatc agcaagcact tttccgaata ccatcagcaa cgccttctga
    34141 tgttgtcgat aatctttttg ggttcggggg gccagttctg taaatgcagg ggagtccata
    34201 aacatgtgcc acaatttagc tacggtcatt atgttgtgga gttttgcttt ttccagttca
    34261 taattttgcc agactttagc tacgctggtt ttccgcactc ttcctagccc tatacttctt
    34321 gtacttcctt cgggtttcca cacgtaactg taaccattcg atctaacccg cggtggcagt
    34381 acattatctt ttttattttt tcttggtctt cccattgttc agcgcctcaa aatcgggttc
    34441 agcagaaacc agttcagatg tttttggcat cgttgtcaga ccgtgtggaa tatccctgcg
    34501 gagaactatg ggttcgtttt tagggccgat tacaaatggg atgccgtgca gtcttaactg
    34561 gtgttgctgt tttgtgtatc gctcgtattt cgtgatctct ttaatctctg ctggagatag
    34621 agttaattcg tacatgtggt cacgtgcctt tacagcatga ccgcggccac tatagctggt
    34681 gggcggcgat atgggttgaa catcaaaaat cagcctgact cgggagcagt ttttgccaga
    34741 tggctgaaac gtatttcgcc tggtaacg
//
LOCUS       DAZKIF010000038        34682 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZKIF010000038 DAZKIF010000000
VERSION     DAZKIF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.038"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            269..421
                     /gene="mokC"
                     /locus_tag="ACHOTW_001848"
     CDS             269..421
                     /gene="mokC"
                     /locus_tag="ACHOTW_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP9843965.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(525..1655)
                     /gene="dnaJ"
                     /locus_tag="ACHOTW_001849"
     CDS             complement(525..1655)
                     /gene="dnaJ"
                     /locus_tag="ACHOTW_001849"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9843966.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1744..3660)
                     /gene="dnaK"
                     /locus_tag="ACHOTW_001850"
     CDS             complement(1744..3660)
                     /gene="dnaK"
                     /locus_tag="ACHOTW_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9843967.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4037..4441
                     /gene="yaaI"
                     /locus_tag="ACHOTW_001851"
     CDS             4037..4441
                     /gene="yaaI"
                     /locus_tag="ACHOTW_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP9843968.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4467..5180
                     /gene="msyB"
                     /locus_tag="ACHOTW_001852"
     CDS             4467..5180
                     /gene="msyB"
                     /locus_tag="ACHOTW_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9843969.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5329..5895
                     /gene="satP"
                     /locus_tag="ACHOTW_001853"
     CDS             5329..5895
                     /gene="satP"
                     /locus_tag="ACHOTW_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP9843970.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5930..6517)
                     /gene="mog"
                     /locus_tag="ACHOTW_001854"
     CDS             complement(5930..6517)
                     /gene="mog"
                     /locus_tag="ACHOTW_001854"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP9843971.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6632..7585)
                     /gene="tal"
                     /locus_tag="ACHOTW_001855"
     CDS             complement(6632..7585)
                     /gene="tal"
                     /locus_tag="ACHOTW_001855"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9843972.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7864..9294
                     /gene="yaaJ"
                     /locus_tag="ACHOTW_001856"
     CDS             7864..9294
                     /gene="yaaJ"
                     /locus_tag="ACHOTW_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP9843973.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            9364..10140
                     /gene="yaaA"
                     /locus_tag="ACHOTW_001857"
     CDS             9364..10140
                     /gene="yaaA"
                     /locus_tag="ACHOTW_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP9843974.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10293..10589)
                     /gene="yaaX"
                     /locus_tag="ACHOTW_001858"
     CDS             complement(10293..10589)
                     /gene="yaaX"
                     /locus_tag="ACHOTW_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP9843975.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10803..12089)
                     /gene="thrC"
                     /locus_tag="ACHOTW_001859"
     CDS             complement(10803..12089)
                     /gene="thrC"
                     /locus_tag="ACHOTW_001859"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP9843976.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12090..13022)
                     /gene="thrB"
                     /locus_tag="ACHOTW_001860"
     CDS             complement(12090..13022)
                     /gene="thrB"
                     /locus_tag="ACHOTW_001860"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP9843977.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13024..15486)
                     /gene="thrA"
                     /locus_tag="ACHOTW_001861"
     CDS             complement(13024..15486)
                     /gene="thrA"
                     /locus_tag="ACHOTW_001861"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP9843978.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15512..15637)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15567..15644)
                     /gene="thrL"
                     /locus_tag="ACHOTW_001862"
     CDS             complement(15567..15644)
                     /gene="thrL"
                     /locus_tag="ACHOTW_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP9843979.1"
                     /translation="MKRISTTTTITTTITITITTGNGAG"
     gene            complement(15858..16544)
                     /gene="yjtD"
                     /locus_tag="ACHOTW_001863"
     CDS             complement(15858..16544)
                     /gene="yjtD"
                     /locus_tag="ACHOTW_001863"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9843980.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16944..17084)
                     /gene="yjjY"
                     /locus_tag="ACHOTW_001864"
     CDS             complement(16944..17084)
                     /gene="yjjY"
                     /locus_tag="ACHOTW_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP9843981.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17180..17896
                     /gene="arcA"
                     /locus_tag="ACHOTW_001865"
     CDS             17180..17896
                     /gene="arcA"
                     /locus_tag="ACHOTW_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP9843982.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17956..19308)
                     /gene="creD"
                     /locus_tag="ACHOTW_001866"
     CDS             complement(17956..19308)
                     /gene="creD"
                     /locus_tag="ACHOTW_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP9843983.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19366..20790)
                     /gene="creC"
                     /locus_tag="ACHOTW_001867"
     CDS             complement(19366..20790)
                     /gene="creC"
                     /locus_tag="ACHOTW_001867"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP9843984.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20790..21479)
                     /gene="creB"
                     /locus_tag="ACHOTW_001868"
     CDS             complement(20790..21479)
                     /gene="creB"
                     /locus_tag="ACHOTW_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP9843985.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(21492..21965)
                     /gene="creA"
                     /locus_tag="ACHOTW_001869"
     CDS             complement(21492..21965)
                     /gene="creA"
                     /locus_tag="ACHOTW_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP9843986.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22176..23045
                     /gene="robA"
                     /locus_tag="ACHOTW_001870"
     CDS             22176..23045
                     /gene="robA"
                     /locus_tag="ACHOTW_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP9843987.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23042..23689)
                     /gene="gpmB"
                     /locus_tag="ACHOTW_001871"
     CDS             complement(23042..23689)
                     /gene="gpmB"
                     /locus_tag="ACHOTW_001871"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP9843988.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23741..24253
                     /gene="yjjX"
                     /locus_tag="ACHOTW_001872"
     CDS             23741..24253
                     /gene="yjjX"
                     /locus_tag="ACHOTW_001872"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP9843989.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(24400..24726)
                     /gene="trpR"
                     /locus_tag="ACHOTW_001873"
     CDS             complement(24400..24726)
                     /gene="trpR"
                     /locus_tag="ACHOTW_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP9843990.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24816..26753)
                     /gene="sltY"
                     /locus_tag="ACHOTW_001874"
     CDS             complement(24816..26753)
                     /gene="sltY"
                     /locus_tag="ACHOTW_001874"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP9843991.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26964..28631
                     /gene="ettA"
                     /locus_tag="ACHOTW_001875"
     CDS             26964..28631
                     /gene="ettA"
                     /locus_tag="ACHOTW_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP9843992.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28738..28905)
                     /gene="nadS"
                     /locus_tag="ACHOTW_001876"
     CDS             complement(28738..28905)
                     /gene="nadS"
                     /locus_tag="ACHOTW_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP9843993.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(28939..30171)
                     /gene="nadR"
                     /locus_tag="ACHOTW_001877"
     CDS             complement(28939..30171)
                     /gene="nadR"
                     /locus_tag="ACHOTW_001877"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP9843994.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30192..31574)
                     /gene="radA"
                     /locus_tag="ACHOTW_001878"
     CDS             complement(30192..31574)
                     /gene="radA"
                     /locus_tag="ACHOTW_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9843995.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31623..32591)
                     /gene="serB"
                     /locus_tag="ACHOTW_001879"
     CDS             complement(31623..32591)
                     /gene="serB"
                     /locus_tag="ACHOTW_001879"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP9843996.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32697..33338
                     /gene="ytjB"
                     /locus_tag="ACHOTW_001880"
                     /pseudo
     CDS             32697..33338
                     /gene="ytjB"
                     /locus_tag="ACHOTW_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            33369..34385
                     /gene="lplA"
                     /locus_tag="ACHOTW_001881"
     CDS             33369..34385
                     /gene="lplA"
                     /locus_tag="ACHOTW_001881"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP9843997.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34417..34680
                     /locus_tag="ACHOTW_001882"
     CDS             34417..34680
                     /locus_tag="ACHOTW_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9843998.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
BASE COUNT         8187 a         9428 c         8655 g         8412 t
ORIGIN      
        1 agataaatta aagttaaata attaaatcat taaaaaatta cgccataaaa atgcgaggcg
       61 tcttccggat tagaatcgat aaaacagaat gggttaaaga gagcgatata atagcggcgg
      121 gtgcttgagg ctgtctgtct caggcattag ctgaacggca gatagagaaa agccccgagt
      181 gatattttac catcaacccg aggcccccta tatgctgaac acatgtagag tgcctcttac
      241 tgaccgtaag gtcaaggaga agagagcaat gaagcagcat aaggtgatga ttgtcgccct
      301 gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta acgagaaaag acctctgtga
      361 ggttcacatc cgaactggcc agacggaggt tgctgttttc acggcttacg aatccgagta
      421 agagcaacgg cggggagtga tccccgccac tttacaggtg ctcgcatatc ttcaacgcac
      481 cctattttta cccaggcctg cccacgggca ggcttttggg gaggttagcg ggtcaggtcg
      541 tcaaaaaact tcttcacacc atcaaagaag ctctttgagc gcgggctgtt gtgctcgccg
      601 gttgggccac cgaagctttc ttgcagctct tgcagcagct gtttctgctt ctcgttcagg
      661 cctaccggtg tttcgacgac aacgcggcac agcaaatcac cctgtgcgcc accgcggaca
      721 gacttgacgc ctttaccgcg catacggaat agcttaccgg tctgggtttc gccaggcact
      781 ttcagtttga cgcgaccatc aagggtcggt acttcgattt caccacccag cgccgccata
      841 gcgaagttga tcgggacttc gcaatacagg ttgttgcctt cacgctcgaa aatcgggtgc
      901 tgtttaacct gaacctgaac gtacagatcg cctgccggtg cgccgtgttc acccgcttca
      961 ccttcgcccg caagacggat gcggtctcca gtgtccaccc ctgccgggat tttaacggac
     1021 agcgttttgc tgcgctcaac acgaccatga ccatgacatt tgttgcacgg atctttgatc
     1081 agcgtaccgc ggccctgaca gtgtggacag gtctgctgca cggcaaagaa accctggcgc
     1141 atctgcacct gaccagaacc atgacaggtc ggacaggtct gcggctgtgt acctggtttt
     1201 gcaccgctac cgtggcaaac gtcacactct tccagagtcg gaatgcggat ctctttggtc
     1261 acgccacgta cagcttcttc gagggtgagc tccatgttat agcgtaaatc agcaccgcgc
     1321 gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc cgaaaacgtc accaaaaata
     1381 tcgctgaagt ctgcgccgcc gccaaaaccg ccgccgccca tgccaccttg ctcaaacgca
     1441 gcatgaccat actgatcgta tgccgcacgt ttttgcgagt cggtcagaac ttcataagct
     1501 tccttgatct ctttaaattt cgcctcggcc tctttgtcac cctggttacg gtccgggtgg
     1561 tatttcatgg ccaggcgttt gtaggccttt ttgatttcac gctcttccgc tgttttggaa
     1621 acgcctaaaa tctcgtaata atcttgctta gccatctttt ttaaattgcc cctagatgaa
     1681 tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt ataattaccc gtttataggg
     1741 cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg acaacatcgt catctttcgc
     1801 gttgtttgca gaagcatcag caccggcagt ctgctgctgg gcatgttgct gctgggcgat
     1861 ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt ttcgcttcga tagcggcttt
     1921 gtcttcacct ttcagagcag tttccagtgc agtcagcgca gactcgatag cagttttgtc
     1981 gtcagccggc agtttgtcgc ctgcttcttc aacctgctta cgggtgctgt gcagcagatg
     2041 gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta cggtcagctt cggcgttagc
     2101 ttcggcgtcg cgtaccattt tctggatttc atcttcgttc agaccagaag acgccttgat
     2161 ggtgatcttc tgctctttac cgctgttttt atctttcgcg gaaacgtgca ggataccgtc
     2221 agcatcgata tcgaaggtaa cttcgatctg cggcatgccg cgcggtgccg ggttgatacc
     2281 atccaggttg aactgaccca gagatttgtt gtcagccgca cgtttacgtt caccctgcag
     2341 cacatggatg gttaccgcag actggttgtc ttcagcggta gagaacacct ggctgtgctt
     2401 ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc acaccgccca tggtttcgat
     2461 acccagagac agcggggtaa catccagcag cagtacgtct tttacgtcac cagtcagaac
     2521 accaccctga acagcagcac cgattgctac agcttcgtcc gggttaacgt ctttacgcgg
     2581 ctctttacca aagaactcag caactttctt ctgaaccatt ggcatacgag tctgaccacc
     2641 aacgaggata acgtcgtcga tatcagatac ggacaggcca gcgtcctgca gcgcaacttt
     2701 cagcggctcg atggaacggt taaccagatc ttcaaccagg ctttccagtt tcgcacgagt
     2761 cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca gtgatgtacg gcaggttaac
     2821 gtcggtctgc tgagcggaag acagttcgat tttcgctttt tctgccgctt ctttcaggcg
     2881 ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga tctttcttga attcttcaac
     2941 cagatagttg atcagacggc tgtcgaagtc ttcaccaccc aggtgggtat caccgttggt
     3001 tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg atttcgataa tagaaatatc
     3061 gaaagtacca ccacccaagt cataaaccgc gatagtacgg ttgccagtac ctttgtccag
     3121 accgtaagcc agcgcagctg cggtcggttc gttgatgata cgttttactt ccagaccagc
     3181 gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg ttaaagtatg ccggtacggt
     3241 gataacagct tcagttaccg gttcacccag gtaatcttca gcggttttct tcattttttt
     3301 cagcacttca gcagaaatct gcggcggtgc cattttctgg cctttaactt cgacccatgc
     3361 gtcgccgtta tcagcagcaa taattttgaa cggcatgatg gaaacatcac gctgtacttc
     3421 ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca aacagggtgt tttgcgggtt
     3481 cgtcactgcc tgacgtttag ccggctgacc aaccagagtt tcaccatcct gggtataggc
     3541 aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc agcacacgag gagtggtgcc
     3601 atccataatc gctacacaag agttggtagt acccaggtcg ataccaatta ttttacccat
     3661 ctaaacgtct ccactatata ttcggtcatc atgtggttgt gagtctgtaa taggggcgaa
     3721 acgtctggtt tcaactgccc aatttcattt ttttgcagac tcactcactg cggttgacta
     3781 ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc aaaaattttt tgtgcttttg
     3841 cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt gacagcaatt ttattcatat
     3901 aaagaatgaa tcgcagtcaa ggtattcatc aaaaaattgt aataaaaaga aaagattacg
     3961 tgcctgaatc ttctctttat cagcagtaaa ctagtgggta ttcatccccc tacctcttcc
     4021 cactaagaga atccttatga aatccgtttt tacgatttcc gccagcctgg cgattagcct
     4081 gatgctgtgc tgcacggcgc aggcaaacga ccataaactc ctcggcgtca ttgcaatgcc
     4141 gcgtaacgaa accaacgctc tggcgctgaa acttcctgtt tgtcgcattg tgaaacgcat
     4201 acaactctct gccgaccatg gcgatttaca gttaagcggt gcatcggttt atttcaaagc
     4261 cgcccgtagc gccagtcaga gcctgaatat tccttcagaa ataaaagaag ggcaaaccac
     4321 tgactggatc aacattaaca gcgataacga caataaacgc tgcgtctcaa aaatcacctt
     4381 ttccggtcat acggtgaact catcggatat ggccacgctg aaaattatcg gcgacgacta
     4441 acgccagaat atagaagcca caaaaaatga atgttaatta cctgaatgat tcagatctgg
     4501 attttctcca gcattgtagt gaggaacagt tagcaaattt cgcccggctg ctcacccata
     4561 atgaaaaagg caaaactcgc ctctccagcg tactgatgcg caacgaactg tttaaatcga
     4621 tggaaggaca tcccgagcaa catcgccgca actggcagct gattgctgga gaattacagc
     4681 attttggtgg cgatagtatc gccaacaaac tgcgcggaca cggtaaattg tatcgggcca
     4741 ttttgctcga tgtttcaaaa cgattgaagc tgaaagccga caaagagatg tctacgtttg
     4801 aaattgagca acagttactg gaacaatttc tgcgtaatac ctggaagaaa atggacgagg
     4861 aacataagca ggagtttctg cacgcggtcg atgccagggt gaatgagctg gaagagctgc
     4921 tgccgctgct gatgaaagac aaattattgg caaaaggcgt gtcgcatctg ctttccagcc
     4981 aactgacccg cattttacgc acccacgcag caatgagcgt acttgggcat ggtttgctgc
     5041 gcggcgcggg gctgggaggc cctgtaggcg cggcactaaa tggggttaaa gcggtcagcg
     5101 gcagctccta tcgcgtgacg attccagccg tactgcaaat cgcctgcctg cgccggatgg
     5161 ttagcgccac tcaggtctga taccggaaac cggggaaaat tttcattttc ccctcaaaag
     5221 atcgtagaca ctgccccact ggctgattat tatgccgcgc cctgaaaaca ctacagttat
     5281 tcagggaaat tatttcacca ttcattcgat gatgattttt gaggaattat gggcaacact
     5341 aagttggcta atccggcacc gctgggcctg atgggcttcg gcatgaccac cattctgctt
     5401 aacctgcaca acgtaggtta tttcgctctg gacggtatta ttcttgccat gggcattttc
     5461 tacggcggca tcgcgcaaat ttttgctggt ctgctggagt acaaaaaagg caacactttc
     5521 ggtttaaccg cattcacctc ttacggttct ttctggctga cgctggttgc gattctgctg
     5581 atgccgaaac tgggtctgac cgatgcgcca aatgcacagt tccttggtgt ctacctgggt
     5641 ctgtggggcg tatttacgct gtttatgttc ttcggcacgc tgaaaggcgc acgcgttctg
     5701 caattcgttt tctttagcct gaccgtgctg tttgccctgc tggcgatcgg taacattgcc
     5761 ggtaacgccg caatcatcca ctttgccggc tggattggtc tgatctgcgg tgccagcgca
     5821 atctatctgg cgatgggtga agtactgaac gagcagtttg gtcgcaccgt tctgccgatt
     5881 ggtgaatccc actaattgtc ttgagatccc cccgctcggg gggatttttt tattcgctaa
     5941 cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac ttctggtgcc gtttcaacgt
     6001 atggcccttc cagcaactga atgcagtacg gtacgctggc aaaaataccg tgtaccacaa
     6061 cgttaccctc agcgtccttc acaccttcca gcgtctcttt aatagacttc ggctgaccgg
     6121 gtaagttaag gatcagcgcc tgtttgcgaa tcacacccac ctgacgcgaa aggatcgcag
     6181 ttggtacaaa atgcaggctg atctggcgca tctgttcacc aaagccaggc atctcgcggt
     6241 ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg cgccgggcca gttccgcccg
     6301 tggtgagcac cagatggcaa ctcatttcat ccaccagctc acacaacgtt tgctcgatga
     6361 tcgcctgctc atcggggatt aagcgggttt ccagttcaaa cggcgtggtt agcgccgatg
     6421 tcagccattc ttccagcgca gggatacctt tatcctgata aacgccgctg gatgcgcgat
     6481 cagagatgga aactaagcca atacgtaaag tattcatcaa gatttttccg tttaaacagt
     6541 ctcgttaaac agaatgatac actgcgaagg gagtgacaga caggcttcag aagaggtagc
     6601 gtgaccgact tcccggtcac gctaagaatg attacagcag atcgccgatc attttttcca
     6661 gtttttcctg gtcaatagca aacttacgga taccttccgc cagtttatct actgccattg
     6721 gatcctggtt gtgctgccac aggaactcgg actcagtgat acgcgccgga cgcgctttca
     6781 cttcgccggt gtaagacagt ttacgttcga tagccccttc gctctcagcc agctctttca
     6841 gcagtgccgg tgcgatggtc agacggtcgc agcctgccag ttccagaatt tcgccgatgt
     6901 tacggaagct tgcgcccata accacggttt cataaccgtg ctctttgtag tactggtaga
     6961 tttcagatac agaaaccacg cccggatctt ctgccggagc gtactctttc ttatcggtat
     7021 tcgctttgta ccagtcaaga atacggccaa caaacggcga gatcaggaac acgcccgctt
     7081 ccgcacaagc acgagcctga gcgaaggaga acagcagggt caggttacag ttgatacctt
     7141 ctttttccag ctgttccgca gcacggatac cctgccaggt agaagccagt ttgatcagaa
     7201 tacgatcgtt gctaatacct gcatcgttgt agagtttgat caggcgtttt gctttcgcaa
     7261 ttgacgcttc tgtgtcatag gaaagacgcg catcaacttc agttgagata cggcccggaa
     7321 ccagtttcag gatttccaga ccaatattta ctgccagttt gtcagtcgcg tccacgatct
     7381 gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac agcatcatca atcaacttac
     7441 ggtattccgg aatctgcgct gcgttaagaa tgagagaagg gttggttgtg gcatcctgcg
     7501 gttgatacag cttcattgcc gcgatgtccc cagtgtcggc cactacggtg gtgtactgac
     7561 gaagggaggt caatttgtcc gtcatgatag tatttctctt taaacagctt gttaggggga
     7621 tgtaaccggt ctgccctgat gatatcacga cgcaacgtca gcgcaaccgc cgacaagacg
     7681 gttatcgcca ggcgagactg tttcggattt ctgacactgc gttgatatcg cccgccattt
     7741 ttaaacaaaa cctcatgtat gctacgcaga agttatcaag tacctcgtag cgtatatact
     7801 tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc gaccggcaat aagagggata
     7861 tgcatgccag attttttctc ctttattaac agcgtccttt ggggatcggt aatgatttac
     7921 ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg gatttgtgca gtttcgctac
     7981 atccgccagt ttggcaaaag tcttaaaaat agcattcatc cacagccagg cggtttaacc
     8041 tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg gtagcggcaa cctggccggc
     8101 gttgcgctgg ctattaccgc cggtggacct ggagccgtct tttggatgtg ggttgccgcg
     8161 tttatcggca tggcgacctc gtttgccgaa tgttcccttg cacaacttta taaagaacgt
     8221 gacgtcaatg ggcagtttcg tggcggaccg gcatggtata tggcgcgcgg gctggggatg
     8281 cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg cctacggcat aattttcagc
     8341 ggagttcagg caaacgctgt tgcccgagct ctgagttttt cttttgattt tcccccgctg
     8401 gtgacaggca ttattcttgc tgtctttgct ctgctggcaa tcattcgcgg tcttcatggc
     8461 gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt actgaccagc
     8521 ctggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc tatttttgag
     8581 agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt aagccaggcg
     8641 attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg gtcgacgcca
     8701 aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg aattgtccag
     8761 atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat gctgatatta
     8821 ctggcgggta acggcacaac ctacatgccg ctggaaggta ttcagcttat ccagaaggcg
     8881 atgcgggtgc taatgggttc ctggggtgct gaatttgtta ccctcgtggt tattctgttt
     8941 gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt ctttttacgc
     9001 ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc
     9061 ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat ggcctgcatg
     9121 gctattacca atttgactgc catattactg ctctcgcctg tggttcatac cattgccagt
     9181 gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg
     9241 gatatcggtc gccagctttc tcgggacgcg tgggatgatg tttcgcagga gtaatcacaa
     9301 ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct gcaaggactg
     9361 gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc gttgaccacc
     9421 acgcgatata cactgccgga gctgttagac aattcccagc agttgatcca tgaagcgcgg
     9481 aaactgacgc ctccgcagat tagcacgctg atgcgtatca gcgacaaact ggcaggtatc
     9541 aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc ccgccaggcg
     9601 attctggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacctt cagcgaagac
     9661 gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg cgtactccgc
     9721 ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga gaatgcccga
     9781 gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa cgaggcgctc
     9841 gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt taaatcggtg
     9901 aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga gaagaacggc
     9961 aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt
    10021 attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga aggttacttc
    10081 tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga gcagcgttaa
    10141 gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcatgcaga
    10201 ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg catcaggcgc
    10261 tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga
    10321 ccgccgtgat gatcatgatg agctttctta tggtgacgcg gcggtggcgg cggtccgtgt
    10381 gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag
    10441 tgaccgccat cccagtaata accgcgatta tcacgatcgc ctatctgtaa ttttactgac
    10501 gggactaacg taatttccgc agcctgcgtt gccatgggag cgaccagaac cagggaaagt
    10561 gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg
    10621 gtcgatatcc ggagattacg cgatgagagc ggcggttgtt attctccgca atcctaatct
    10681 ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttctc tccatatttt
    10741 cttcacaaaa aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga
    10801 tgttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgc
    10861 gaaagcaagg gtaaatcggc acgttctgcc agctcttttg gcagatccaa cgtttcaccg
    10921 agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca
    10981 tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc gtgcggctcc
    11041 gaagtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg
    11101 gcggcataac ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc
    11161 ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg
    11221 ggtgaccact gaccgtcatg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca
    11281 ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt caaatcgccg
    11341 aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg cagctgcgca
    11401 actgcttcaa agtagtagca aatctgcgcc agcaaccggc taatgttaat cgagttagct
    11461 gagtttaacc ccagcgccac tttcagctct tcatcatcaa acgcctgctt caccagcgcc
    11521 tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caatgtacag
    11581 aacagttttt cttgcagtgg actgattttg cctcgtggat aaaggataac cactttcaca
    11641 ttcggtaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga ggtcgcggtc
    11701 agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg
    11761 ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt
    11821 tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt
    11881 tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa
    11941 tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc gtgcggaaaa
    12001 aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctca
    12061 ttgtgatctt taagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta
    12121 tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag
    12181 tcggcaacgc gctgggcggt atccggcttg tcacacagag caaacaaggt cgggccggag
    12241 ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag
    12301 ccaggcagta accgttcacg gtagggttct gcgataacat ctttcatcag cttcgcggca
    12361 agctcaggct gacgggaata gcaggcgtga atgaagcctg ccaagtgtcg cccgtgcgca
    12421 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc cgtcgagact
    12481 ttaatccccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
    12541 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
    12601 tcgtaatgaa tgctgccgga gattcgtcct tccagctcgc ccatcaaagc cagcaaacgg
    12661 gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca tcagagccgc gaccaccgaa
    12721 caggcgctgg agcctaagcc cgaaccgatc ggcatattct tttccagagt catcgccact
    12781 ggaatttgct tgcccagctc ctggcaaaaa cgctcccagc actgataaac gatattttcc
    12841 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
    12901 gccgactcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt caccgccgcc
    12961 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
    13021 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccggcagc
    13081 ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
    13141 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattaccatc
    13201 cacttcggca atcttcacgc ggcagacgcc atcttcatca atattgccaa catagcgcaa
    13261 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
    13321 cagattcgcc ataaaagcgg caacatcacc ctcagcgtta aactctgcgg gcagcacagg
    13381 ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga gaattaatag
    13441 tttacgcgct acatccatac cagaaagatc atctcgcgga tccggttcgg tataacccat
    13501 ttcccgcgcc agcgtagtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
    13561 ataagaaagc gaacctgaaa gaatgccgga gaacttcatc aattcatcac cagcattgag
    13621 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
    13681 tttacgccgc gatttttcag ccgcatgacg caacagatgg tagtaatcca tcgacgaggt
    13741 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg
    13801 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
    13861 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg ccagttcttc
    13921 ctgccagttt tccagattta ggccatgcac attggtgagc agagccttcg agttagcaac
    13981 accgcagaca cgtaagtcga tatgtttatt cttcaaccag ctttgctgac gcttcagttg
    14041 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
    14101 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
    14161 cacgacagag attgagcgtt cagaagatcc ctgagcaata gcgacaatgt tgatattggc
    14221 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
    14281 taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta agccttcttt
    14341 cagttccagg tagaactctt cctgcattgc ccgttcagct cgcacacagt cgctttgcgg
    14401 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
    14461 ggcgcgtgac attgcagcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg
    14521 gccggaaacg ctgaacattg ccatattatt cagattggaa atgcccttga ccggtaattc
    14581 gtcttcatca cgactggcac caatgagcgt acctggagct tgaggatttc cggtattttt
    14641 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
    14701 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgatttca acaacctcgc
    14761 atcgggcacc tgacgcgggt cgcaggtata taccccgtca acgtccgtcc aaatctcgca
    14821 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc
    14881 aagtaccacc agttcgcctt tttcattacc ggcggtgaaa cctgccatca gcaccatgtg
    14941 atcagccgga atacgacttg ccgcaatacg gcgggtggac tctgcaatat cgacagtaga
    15001 ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga tcgataacgg taacgttgtg
    15061 accgcgcgct tctaatacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
    15121 cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat gcaggacatg
    15181 ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg ggaatcccgg
    15241 ctgggcggcg gcgagtcccg tcagaagttc ggcaaaaata cgttcggcat cgctgatatt
    15301 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
    15361 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
    15421 atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
    15481 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa gcccgcactg tcaggtgcgg
    15541 gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt tacctgtggt aatggtaatg
    15601 gtgatggtgg tggtgatggt ggtggtggtg ctaatgcgtt tcatggatgt tgtgtactct
    15661 gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag tgacctaagt
    15721 caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgctg tcagagactc
    15781 tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa gctgaaaaat
    15841 acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag caaacggtgc
    15901 aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gttgtaacca gtcgaccagt
    15961 tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tggctcgttc gcgtaaagct
    16021 tgcagttgat gttggtctgc cgttgtgtca cttttcgtcg gttgttgtat taatattgct
    16081 aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata atccgccacc
    16141 atcggcacac cagtaagaac gtcagccaac gctaactctt cgttagtcag cccggaatct
    16201 tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc taacagcggc
    16261 accagttcta ctggcgtagc gtagtaatga tatttcgccc gactgcgcgc agtggtggcg
    16321 acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt aatattatca
    16381 ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg tgcctgacta
    16441 tcgacaatcc gcagttcgct aaaccccatc gttttcattg cccgcgccgc tgccccaata
    16501 ttttctgctc tggcgggtgc gaccagaata atcgttatac gcatattgcc actcttcttg
    16561 accaaatgac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg aatttacaaa
    16621 ttcttaacgt aagtcgcaga aaaagccctt tacttagctt aaaaaaggct aaactatttc
    16681 ctgactgtac taacggttta attgttaaaa aatgctacat atccttctgt ttacttagga
    16741 taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag ttgtatgatg
    16801 caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac gatgatttca
    16861 tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg
    16921 ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa tggttaggat gacagccgtt
    16981 tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg
    17041 ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt
    17101 taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt
    17161 ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt
    17221 aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac
    17281 agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga
    17341 tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa
    17401 tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga
    17461 aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc
    17521 acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt
    17581 tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttctt tgatcggccc
    17641 tgatggcgag cagtacaagc tgccgcgcag tgagttccgc gccatgcttc acttctgtga
    17701 aaacccaggc aaaattcagt cccgtgctga actgctgaag aaaatgaccg gccgtgagct
    17761 gaaaccgcac gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc
    17821 tacgccggat acgccggaaa tcatcgccac cattcacggt gaaggttatc gcttctgcgg
    17881 tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa acggcgcttt ttagcgccgt
    17941 ttttattttt caaccttatt tccagatacg taattcatcg tccgttgtaa cttctttact
    18001 ggctttcatt ttcggcagtg aaaacgcata ccagtcgata ttacgagtca caaacatcat
    18061 gccggccagc gccaccacca gcacactggt tcccaacaac agcgcgctat cggcagagtt
    18121 gaggagtccc cacatcacac catccagcaa caacagcgcg agggtaaaca acatgctgtt
    18181 gcgccaacct ttcaataccg cctgcaaata aataccgttc attaacgccc caatcagact
    18241 ggcgattatc catgccacgg taaaaccggt atgttcagaa agcgccagca agagcaaata
    18301 aaacatcacc aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac gttgcgcggt
    18361 gagcgtttca aaaacaaaga acgccataaa agtcagcgca atcagcagaa tggcatactt
    18421 agtcgcccgg tcagttaatt ggtattgatc ggcgggcgtc gtcactgcga cgctaaacgc
    18481 ggggaagttt tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga gattattagc
    18541 aaaccagctg ctttgccatt gcgcctgaaa acctgactca ctaacttccc gtttggctgg
    18601 tagaaaatca cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca tttcgctatt
    18661 acgccccgca ggcaccacag aaagatcgcc ggtaccgctt aaattcagtg ccatattcag
    18721 cttcaggttc tgcttccgcc agtccccttc gggtaaaggg atatgcacgc cctgcccgcc
    18781 ttgctctaac ccgatgccgg gttcaatagt cagcgccgtt ccgttaactt caggcgcttt
    18841 caccacacca ataccacgcg catccccgac gctaatcaca ataaatggct tgccaaaggt
    18901 gatatttggc gcgtcgagtt cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa
    18961 atcactgtgc cagacctgac cggtataaat ccctatcttg cgttcttcca cgttctgatt
    19021 gccatcaacc atcaatgact caggtaacca aaaatggata aaacttcgtt tccgctccac
    19081 ggttttatcc tcttcctgca ccgtataaag ctcggtcaca ggaatagcga taagaggccc
    19141 aacgagtttt tgcggcccgc tggtactttg gcgaatcgca tcttccacat cgctacggta
    19201 atcggcacgt tcgacaatca cctgccgaat cagcattatc ggaatcaaca acagcaatac
    19261 tgcaccaaac agggtggtca ttttccagaa caggggggat ttcaacatag tcttctcctt
    19321 tgcaatggca gcaggatacg aaggctatgt gggaagaatt tgaagctatg tgaagtgacg
    19381 gtgaagccga agcgaggcca gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc
    19441 gttaaacaaa cgggcgacct cactgacaaa cgccaacccc agaccgctgc ttttttgccc
    19501 atttgcacgc ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat
    19561 accactaccg gtatccagca ccttaagggc gacgtgttcc tgatccactt cggcgcttag
    19621 cgttatgcga ccgctcttgg gggtaaaatc gatggcgtta tccagtaaat tccccagcgc
    19681 ctgctccagt aacgccggtt cagcagcaac gttaacctca gtaggcataa catgcaaagt
    19741 gatgtttttt tctgccaact gcacggtgcg cgcttcgctg acgcggcgaa ataatgccgc
    19801 cacatcaaca gcagtcagaa cgacttcctg acgattctcc agtcttgcct ggcgtagtaa
    19861 cgtttctacc aatgcctgca tacgcgcatt ttgcgtcaga atgttgtcgg taaaacgagc
    19921 caccacttcc ggtggcggac cttcgcgtaa aatttccgcc gcgccacgaa tcgccgccag
    19981 tgggcttttt agctcatggg ttaacgcata aacatactgc tcaatatagt ttttcccttc
    20041 cagcttcacg cgcatacttt ccagcgcctg cgcaagctta cgcaactcgc tactgccgag
    20101 ttcaggaaga ggaacgggct tattgtcagt gacggaatcg gcatagcgag tgagccgggc
    20161 aatggagcgg ttgatccacc aaaccatgcc tgcgccaatc accagtgcaa tccccagcaa
    20221 aatggcgctg gcccataaaa ttcgccgctc gctgcgctta atgaccggag ccatcgccgc
    20281 gttcggtttg cctacgctca aaacgccaat aagccgcgag ccgtccataa tcggcgcggc
    20341 aacatacatc accgaacttt cgggatcggc aggattttgc aacgtgctgc gcgcaccata
    20401 ctgaccacgc aacgttagcc agacgtcatt ccagcgcgaa taatcctgcc caacggcttt
    20461 atttgccgaa tcgaacaata ctttgccctg cgcatcggtc atatagacat gatattcatt
    20521 gcgcactttg ttaatgccac cgatattagc gcgaaacggg cgatgttgta gctgattaaa
    20581 cgcctgcgcc agttgcccat gcgttgggtc cccagagagc aaatcgggac gcgccagctc
    20641 cgccagcaac gttgcggtgt cgatcagcgt cccctccgtt gctcttcgca cgcccggttt
    20701 aacttctttg acaaaaatag ccagtacgaa ccaggccgcc accgccacca gtaaaaaata
    20761 gcccagcaac aaccgcatgc cgatacgcat tacaggctcc tcaggctgta tcccatgccg
    20821 cgatgagtat taatcggtga aagatcgggg ttgatggcgc gcagcttggc acgcagcgtt
    20881 ttaatgtggg tatcgacggt gcgatcgtag gtgtcctgcg catcttccca cacgatatcc
    20941 atcagttgct ggcgggacca gacgcggcct ggcgacttga gtaacgtctt cagcaataaa
    21001 aactcatacc gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc cgcgggttca
    21061 ttcaattcaa aatgaccaat acggatgacg ggagacggcg tcgagaactt cttcacccga
    21121 cgcagcaagg tgcgcaccct agcgcacact tcgcggggtg aaaacggttt agccacatag
    21181 tcgtcagcgc caatttccag cccaagcagg cgatcgacct cttcacttcg ggccgtcagg
    21241 aacagtacag gtaacgccgg atggagcgcc agtaactggc ggcacaattc aaagccactg
    21301 atatcaggca aaccaacgtc gagaatcatg acatcgggaa cctgctggcg agctttatcc
    21361 agcaccggca agcctcgctc aaagacctcg acggcaaaac cttcctgctg caacatgtag
    21421 accagcgtgt cggctatccc ttgctcatct tccactaacc agaccgtttc ccgttgcata
    21481 aatcgcctct gttattgtcg ccacggcatg acaggaaccg cgctaatcgc gtttttcggc
    21541 gaaccttcta caactttgtc ggagtaagcc agataagcga gcgcgttgcg tttggcgtca
    21601 taaaagcgca cgacctgtaa cgacttaaag accagggacg tgcgtttttt gaatactacc
    21661 tcgccctgag ctttgccgtt tttaatacga tccgacagtt caatcggccc gacttgctga
    21721 caagaaatag ccgcatcgga ggtatcttcc gccagaccca atcccccttt aataccacct
    21781 gttttcgccc ggctcacata acaggtgaca tttttcacat cgggatcgtc aaaggcttcc
    21841 acaacaattt tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga accaatctct
    21901 tctgcatgag ccaatggccc cagcattatc aggcttaaag acaggatcaa atgcttgtat
    21961 ttcatattgt taccattttt atctattaca cgagcaatta gttcgtcacg gtaaataaca
    22021 tattgattaa gaacaaaatc tcaatacttt tatttccgtc aagacctaaa acatactcta
    22081 ctaagaaaaa aacactgaat gctaaaacag caaaaaatgc tattatccaa ttacctgatg
    22141 tcaggtgctc gttgttgaaa ggatgaggat attttatgga tcaggccggc attattcgcg
    22201 accttttaat ctggctggaa ggtcatctgg atcagcccct gtcgctcgac aatgtagcgg
    22261 cgaaagcagg ttattccaag tggcacttac agagaatgtt taaagatgtc actggccatg
    22321 ctattggcgc gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga
    22381 ctgcgcgtcc gattctggac atcgcgctgc aataccgctt cgactctcaa cagacattta
    22441 cccgcgcatt caagaagcag tttgcccaga ctcctgcact ttaccgccgt tctcctgaat
    22501 ggagcgcctt tggtattcgc ccgccgctgc gtctgggtga attcactatg ccagagcata
    22561 aatttgtcac cctggaagat acgccgctga ttggtgttac ccagagctac tcctgttcgc
    22621 tggagcaaat ctctgatttc cgccatgaaa tgcgttatca gttctggcac gattttctcg
    22681 gcaacgcgcc gaccattccg ccagtgctct acggcctgaa tgaaacgcgt ccgagtcagg
    22741 ataaagacga cgaacaagag gtattctata ccaccgcgtt agcccaggat caggcagatg
    22801 gctatgtact gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg atgtttacct
    22861 atgaaggtct gggaaccggc gtgcaggagt ttatcctgac ggtatacgga acgtgcatgc
    22921 caatgctcaa cctgacgcgc cgtaaaggtc aggatattga gcgatactac ccggcagaag
    22981 acgccaaagc gggagatcgc ccaattaatc tgcgctgtga actgctgatt cctatccgcc
    23041 gttaacgctg cagctcatct aatgcagggg cgtccagatg cgagatgtcc cctgcggttt
    23101 caacgaccca gccagacgcc agccacaggc tttcctgata atccacgcgc gaaatagaac
    23161 agttacgcag acgtaagcgg cgctctgccc atgctggtaa tccgagaatc gtactcacca
    23221 gacatcccag tgcaatacca tggctcacca gcagcggtcg gcttccctgc ggtaagtccc
    23281 ggcaagattc cagtgccgca ttgacgcgat cgctgagctc ctgcattgac tcgccttcag
    23341 gaatacgccc atcaacggtg ccattgacca gctgccgacg ccagttctct tcttcttcgg
    23401 tcagagaatc gatatgtctt ttttccagca cacccatgtt taattcacgc aggcgagaat
    23461 caaagatgat gtcacagccg caggcctggg cgatgatttc cgccgtacgc cgggtgcgtc
    23521 ctaaatcgct actgatgata tgcgtaatgc caagctcttt ggcacgcgtc gccacctgca
    23581 tcgcctgttg ctcacctttg gcggtcagcg ggctgtcaga ctggccctga atacgtcgct
    23641 cggcgttcca ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc
    23701 gtatactgct gtaacgttaa ttttctgagt aatgctgatt atgcaccaag ttgtctgtgc
    23761 gaccaccaat cccgctaaaa ttcaggccat tctgcaggca tttcacgaga tcttcggcga
    23821 aggatcctgc catattgcat ccattgccgt cgagagcggt gtaccggaac agccctttgg
    23881 cagtgaggaa acgcgcgctg gcgcacgaaa ccgggtagcc aatgcccgcc gtttacttcc
    23941 agaggctgat ttttgggtgg cgattgaagc tggcattgat ggcgacagca ctttcagttg
    24001 ggtggtgatt gaaaacacca cccagcgcgg cgaggcgcgt tcggcaactt tgccgttgcc
    24061 tgcggtgata ttggaaaaag tacgtgaagg tgaggcgctt ggtcccgtga tgtcgcgtta
    24121 taccggtatt gatgagattg gtcgtaagga aggggcgatc ggcgtgttta ccgccgggaa
    24181 actcactcgc gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc cgtttcataa
    24241 tgcggtgtat taagcgcctg atgcgacgct gccgcgtctt atcatgccta ccaaacatat
    24301 tgaaattacg ggtatttgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc
    24361 acgatgcctg atgcgccacg tcttatcagg cctacaaaat caatcgcttt tcagcaacac
    24421 ctcttccagc cactggcgca gctccacggg cgcggctttc aggctgttag atccacgcgt
    24481 aatcgtcgcg atgcctgcgc cgagttcatt ttttaactca cgctggctca tttcgccgcg
    24541 caacagctct tcgacaatac gcacgcgagt ccccaacgct tcgcgctcat ctggcgtcag
    24601 catcaggttt aacaacggta aatggagatc gttttggtag gcattcttaa gcaggtcgac
    24661 aaaacgtaac cactcctggt gacgctgttc tgccatcgct gctgaatagg gtgattgttg
    24721 ggccataata tgtcgccatt gttagcgggg gaagcaaaat gcctcccccg gttgtactcg
    24781 ctaaagagta cgatagcata tcataaacgt gcggatcagt aacgacgtcc ccattccgtg
    24841 gcgctcatca acgtcggttt atcccccatg aaatagcggt agtaagcgtc ataagccagc
    24901 acgttcttca cataaccgcg cgtctcggag aacggaatac tctcgacaaa tgccacagcg
    24961 tcgatacgcc cggcgctgtt gccaagccag gttcgcaccc gtccaggtcc ggcgttataa
    25021 gctgctgagg agaaaatacg gttattacca aattgctgat aaacatattg caggtaactg
    25081 gtgccaatgt tgatattcgt ttccggatcc agcaattgcc cagggctgct ataaccagga
    25141 atagagaaca tcttcaccgt atgggtcgcc gtgcccggca taatctgcat caggccgctg
    25201 gcccctaccg gtgatttcac tttcggattc caggcgctct cctgacgggc aatcgccatc
    25261 gcatagcttt gcgggatctc cttcccactg gtgtaacgtt tgaaaagatc gttgtaagcc
    25321 agcgggaatc gctcttccag atgatcccaa agcttcccgg cgatcgttgc ctgaacgcta
    25381 agatcccacc attggttgtt gaaagcatac cgcgccagtt gagcctgctc tgtttttgac
    25441 ttgctcttca ccagattggc ccactcgcta cgcgcggtgt tatcgagatt ccagtacatc
    25501 aactcgcgca cgcgcgccat ctccggcccc tgagtcaggg cgctgtcaac attctgcggc
    25561 gctttatcaa tcttcagctc atactcttcg ccgatgcgtt gtgcagcaac catcgggtag
    25621 aaaccacgct gttgcatgag ttgatgcaaa atctcttttg cttcagcttc acgtccgcgt
    25681 tccagcaata aatccgcctg ccagtaacgc cattcatctt tctctttcgc ttccataggc
    25741 agacgcgcca gccaggtatt caggccgcga cgatcgccag tgccaagcgc cattcgcaca
    25801 cggcgttcaa taagcgaagt agattgcgag cgcataatgg cgtcatcgcg ccatttcgcc
    25861 tgctcgtcgg tgacatcgtt gcccatcaaa cgccaggcga cgatatcgcg cagctcctga
    25921 atctgatctt cattaagctg ctgcgcctgg gcaagcgatg ggatcatcag ccgtgcattt
    25981 tcagcatcct gccgcgccac actggcaaac gccaccgccg ccatttgacg ggtaaaatcg
    26041 gtcgctccgg ttgtacgcgc gaaggtcagt accgtattgg ggttgttcgc cagtgaaatg
    26101 attgccgagg cgatagtctg gtaatcggca ggcatctgcc ctgccagcac tgttaccagg
    26161 ccagtgttac ccgctttcat cgccagacgg atacgctcta aatacgccag cggatcttgt
    26221 ttacctgacg cacgccagac gctaaataac ttgtcacagg cgttaggctg gctcttgccg
    26281 gttagccaca gctctttcgc cccttgccag gcttcttcac tctgcccggt gttccatttc
    26341 gcatagtagt aattacattg tgcttcggta gttccgggct tttccgggct aaaggctaac
    26401 aggccacgcc agtcttcacg ccgcgccagt tcattgacga aacgagattg cagcgtgcga
    26461 gcgggaggaa gcgtggggtt agcgcgaaca aagttagtga ccgtcaccgc cggttgattc
    26521 atcaggtcat cggtgatctg gcggtattcc aggtagggat aaagcggata atccttcagt
    26581 ccaggcatca tttgttcgac cacatccatt tgtcgattat cccaggcctg cttgatttgc
    26641 gcgtaacgac tacgctgctc atccagtgag tcggctcgcg ccacgctgct gaccgtcagc
    26701 agacagacac cggcagccaa cagccgccag gtaacttgtt tggctttttc cacaagcgca
    26761 tcctctaagt gtaaatacat caatgcagcg tcatgccgcg taatagattt atgctaacca
    26821 gtcattgccg tttacgccac gttacggaca cttttttact tttactgcga gggtgatcga
    26881 ccggctggga ttaggcgggg aaaaaggcta cacttcgcct ttgaaaactc atcatcacga
    26941 taaacataga ggcgaagtcc aacgtggctc aattcgttta taccatgcat cgtgtcggca
    27001 aagttgttcc gccgaaacgt catattttga aaaacatctc tctgagtttc ttccctgggg
    27061 caaaaattgg tgtcctgggt ctgaacggcg cgggtaagtc caccctgctg cgcattatgg
    27121 cgggcattga taaagacatc gaaggtgaag cgcgtccgca gccagacatc aagattggtt
    27181 atctgccgca ggaaccgcag ctgaacccgg aacacaccgt acgtgagtcc attgaagaag
    27241 cggtttcaga agtggttaac gccctgaaac gcctggatga agtgtatgcg ctgtacgccg
    27301 atccggatgc cgattttgac aagctggccg ctgaacaagg ccgtctggaa gagatcattc
    27361 aggctcacga cggtcataat ctgaacgtac agctggagcg tgcggcggat gcgctacgtc
    27421 tgccggactg ggacgcgaaa atcgctaacc tctccggtgg tgagcgtcgt cgcgtggcgt
    27481 tgtgccgcct gctgctggaa aaaccagaca tgctgctgct cgacgaaccg accaaccacc
    27541 tggatgccga atccgtggcc tggctggaac gcttcctgca cgacttcgaa ggcaccgtgg
    27601 tggcgattac ccacgaccgt tacttcctcg ataacgttgc gggctggatc ctcgaacttg
    27661 accgcggtga aggtattccg tgggaaggta actactcctc ctggctggag cagaaagatc
    27721 agcgtctggc gcaggaagct tcacaagaag cggcgcgtcg taagtcgatc gagaaagagc
    27781 tggagtgggt acgtcaggga actaaaggcc gccagtcgaa aggtaaagca cgtctggcac
    27841 gctttgaaga actgaacagc accgaatatc agaaacgtaa cgaaaccaac gaactgttta
    27901 ttccacctgg accgcgtctg ggcgataaag tgctggaagt cagcaacctg cgtaaatcct
    27961 atggcgatcg tctgctgatt gatgacctga gcttctcgat cccgaaagga gcgatcgtcg
    28021 ggatcatcgg tccgaacggt gcgggtaaat cgaccctgtt ccgtatgatc tctggtcagg
    28081 aacagccgga cagcggcacc atcactttgg gtgaaacggt gaaactggcg tcggttgatc
    28141 agttccgtga ctcaatggat aacagcaaaa ccgtttggga agaagtttcc ggcgggctgg
    28201 atatcatgaa gatcggcaac accgagatgc caagccgcgc ctacgttggc cgctttaact
    28261 ttaaaggggt tgatcagggt aaacgcgttg gtgaactctc cggtggtgag cgcggtcgtc
    28321 tgcatctggc gaagctgctg caggttggcg gcaacatgct gctgctcgac gaaccaacca
    28381 acgacctgga tatcgaaacc ctgcgcgcgc tggaaaacgc cctgctggag ttcccgggct
    28441 gtgcgatggt tatctcgcac gaccgttggt tcctcgaccg tatcgccacg cacattctgg
    28501 attaccagga tgaaggtaaa gttgagttct tcgaaggtaa ctttaccgag tacgaagagt
    28561 acaagaaacg cacgctgggc gcagacgcgc tggagccgaa gcgtatcaag tacaagcgta
    28621 ttgcgaagta atgcgtaaaa tgccggatgc ggcgcgaacg ccttatccgg cctacaaaac
    28681 cagcaatttc aatacgttgt aaaactgtag acctgataag acgcggtaag cgtcgcatca
    28741 ggcattgagc accgaatgcc ggatgcggcg tgaacacctt ttccggtcta caacagcctt
    28801 agcatcctca atttcgtggc gggccacccg tgcaggtttt ttcaaacctt gtttaatctc
    28861 gacagcctct tccagagagg ttttcaactc atcaaaaaaa ctcattttat acctcgcctt
    28921 tgagcagttt catcacggtt atctctgctc ccccatcatc tcccgcacca gctccacgca
    28981 gcgcaggaaa cggctgtcgt aatcttcctc ttcaacccgc acaaattcga tattgttctc
    29041 ttccagcatc tccaccagca gattctggaa ctctttacga tccaccgaac tgccgaggct
    29101 gcgtaaacca tccaccaccc acggcgtgtt gttctccagc aggatcacca gatcgaaacg
    29161 gtattcatca atcagcgcct gcacgaacgg atgttcccgc ccttcgtact ttttgcagaa
    29221 cgcctgagtg gtgacaaaat cggtatcgat aaatgccact ttattggcat atttcaccgc
    29281 aaaatcaatg tattgagcgt ggcccagcgc gattttatcg tagtcagaat actgcaatgc
    29341 gatctcatca ccgccgaggt gtgaaaagac ataatcgcgg ccatattccc acgcactggt
    29401 ggtgttgaag atattggcaa gtttgtttac cagggtggat ttaccgctcg actcgccgcc
    29461 aaggatcgcc acggtacgca caaaaaacgg cttcacttcg gtaggaatat attcccagta
    29521 gcggaacggg ttttcgcgga tctgcgcacc gctgatactc ataaaggtac gtttcggatc
    29581 gaccagcacc gtctcgatcc ccagatgttc catatactgt ggcgcatcgg cttcttccga
    29641 ggtgtagatc agatccggct ggatcccctt ttcagccata aactttttga taccgttgct
    29701 ccacacatcc cagccgtgcg gatacggctc catgccctct tcgttgaaag catgaatgcg
    29761 aatatttttc tgatatttaa aagtttgcaa taaccagcgc agacgatccg gcacggtcgg
    29821 ctgctgcgac atggcactgt cttcgaacaa cgcgcggtca cgggtatcgt caaaacccat
    29881 aatgatatgc agctcgtcaa cctggctaca ggcgcgctgg ataaggtaga tatgtccggt
    29941 atgcagtggg tagaacttac cgaatacgac accgatcgtt ttcttctgcc ggggaaactc
    30001 aagccccaaa aaacggtgca acgcctccag cttttgcgcg ctggggcttt tgattttggc
    30061 attcagtaac tggcttaaat accctttggt cataccgctg gcatcagcta cctgctgtag
    30121 cgtgcagccc tgttgcttga tggcagtttt caggtaatca aatgacgaca tatctccctc
    30181 cgtatttctc attataagtc gtcgaacacg ctaagcgcgt cggagagttt tttaacgcca
    30241 aaaatctgca tcccttccgg cgcttttttc ggtacgttag ccgccggaac aatcgcccgg
    30301 cgaaaaccgt gtttcgccgc ttcagagatt cgttcctgac cgctgggcac cgggcggatc
    30361 tcccctgcca gcccgacttc accaaacacc accagatcct gcggtagcgg tctgtcacgc
    30421 aggctggaaa ccatcgccag cagtaacgct aagtcggcgc tggtttcggt tacctttacg
    30481 ccgccgacca cgttcacaaa cacatcctga tcggccattt gcagaccacc gtgacggtgc
    30541 aagacagcca gcaggattgc cagacggttt tgttccagcc ccactgccac gcggcgcgga
    30601 ttcgccatca tcgagtgatc gaccagcgcc tgaatctcca ccagcaacgg acgcgttcct
    30661 tcccacacca ccatcacgga gctgccggag gtcacttcat ctccgcgact taagaaaatt
    30721 gccgaagggt tgctgacttc acgcagcccc tgttcggtca tcgcgaagac gcccagctca
    30781 ttcaccgcgc cgaagcggtt tttatggctg cgcaaggtgc gaaaacggga gtcggcatcg
    30841 ccatctaaaa gcaccgaaca gtcgatacag tgttccagca ctttcgggcc agccagcgaa
    30901 ccatctttgg ttacgtgccc caccatgaca atcgccacac cgcgcgtttt ggcgaagcgt
    30961 gtcaaataag ccgccgtttc acgcacctgc gccacgctac caggcgatga ctgtacatcc
    31021 gccatatgca tcacctggat cgagtcaatt accatcagct tcggttgctc ttcttcggca
    31081 atcaggcaga tctgttcgat gctggtttcc gacaacatat tgagattgtc agtcggcagg
    31141 ccaaggcgat gagcgcgcat tgccacctgt tgcagcgact cttcgccggt gacatacagc
    31201 gttttcatct gctgggccag tttgcacagc gtttgcagta gcagcgtgga tttccccgca
    31261 ccagggttac cgccaatcag aatggcactt cctggcacca cgccgccgcc tagtacgcgg
    31321 tcgaactctt taaatccggt ggaaaaacgc ggcagctctt caaggctgat atcggagagt
    31381 ttctggactt ttgccacccc ggcgctaccg gcatagccgc tgagacgctc gttacgcgcc
    31441 accgtaggcg acgcagcaag acgcacctcg gtgatggtgt tccaggcatg acaggcactg
    31501 cactgcccct gccagcgcgg ataatcggcc ccgcattcat tacaaacaaa ggcgcgtttt
    31561 ggagcttttg ccacggtgta cctcgttaat gctgtgccgc ccgcaggatg gcgggcgagc
    31621 aattacttct gattcaggct gcctgagagg atgcagaata cccccatcag gtcagcgtga
    31681 cggatggtga cttccgcctt ttcattcact tttggcttgg catggtaggc aatccccagc
    31741 cctgccgctt tgatcatcgg caggtcattg gctccatcgc caatcgccac ggtctgcgcc
    31801 agcgggattt catactcctg cgcgaggcga gtcagagttt tcgctttgta ctgcgcgtct
    31861 acgatgtcgc cgatcacatt gccggtaaat ttaccgtcca tgatctccag ttcattggct
    31921 accacggctg tcaggcgcag cttgtcgcgc aggtattcag caaagaaagt aaagccgccg
    31981 gaggcaatcg ccactttcca gcccagcgtt tccagcttga gcaccagttg cgttaagcct
    32041 ggcatcagcg gcagattttc acgcacctgt tgcagaatat tggcgtcagc gcctttcagc
    32101 gtcgccacgc ggctgcgcag gctggcggta aaatcgagtt cgccgcgcat cgcccgttcg
    32161 gttacttccg ccaccatctc gcccgttccg gccagtttgg caatttcatc aatacattca
    32221 atctggatgg cggtggaatc catgtccatc accagcaaac ccggcgtgcg caggtgcggg
    32281 atttttccca gcggcgcgac atccagctgc gcttcgtggg ccaggcgtgt agcccgtgcg
    32341 gtgagtgaac ctgccagacg aatcacctga taatcttcca cgcaccaggc ggcaacaatc
    32401 accatcgccg cacccagttt gctctggtat tgggtcagac gctgtttatc cagcccacga
    32461 ccatacagca gccagccgct acgacctgcg tggtaatcca gtggcatcac ttcatcacca
    32521 cttaatgaaa gaggcagacc cggccataaa gagacatctt caggcaggtc gcaccaggta
    32581 atgttaggca ttaaggctcc tgtaaaatcg ttcgaagcag ggaaaataac gcatgaggct
    32641 accttgtatc cattgcttct ggcaacatta agtctcaaat tttcaaaggg tggaagatgg
    32701 ctcgcacaaa actgaaattc cggctgcatc gggcagtgat tgtcctgttc tgtcttgcct
    32761 tgttagtggc actgatgcag ggggcgtcat ggtttagtca aaaccaccag cgacagcgta
    32821 atccacagct ggaagaactg gcccgcaccc tggcgcgtca ggtgacgctg aacgttgcac
    32881 cgctgatgcg taccgactca ccggatgaaa aacgcattca ggcgatcctc gatcagttaa
    32941 cggatgaaag ccgtatcctc gacgcgggtg tgtatgacga acaaggcgat cttatcgcac
    33001 gttctggcga aagcgtcgaa gtgcgcgatc ggctggcgct cgacggtaaa aaagcaggcg
    33061 gctattttaa ccagcagatt gtcgagccaa ttgccgggaa aaacggaccg ctcggctatc
    33121 tgcgcctgac actcgacacc catacgctcg ccaccgaagc ccaacaggtg gataacacca
    33181 ctaacatttt acgcctgatg ttgctgctct cactggcaat cggcgtagtg ctgacccgca
    33241 cgctgctaca gggtaaacgc acccgctggc agcaatcgcc cttcctgtta accgccagca
    33301 aaccggtgcc ggaagaggaa gaaagcgaga aaaaagagtg ccccattact acaagaaagg
    33361 aaatcgttat gtccacatta cgcctgctca tctctgactc ttacgacccg tggtttaacc
    33421 tggcggtgga agagtgtatt tttcgccaaa tgcccgccac gcagcgcgtt ctgtttctct
    33481 ggcgcaatgc cgacacggta gtaattggtc gcgcgcagaa cccgtggaaa gagtgtaata
    33541 cccggcggat ggaagaagat aacgtccgcc tggcgcggcg cagtagcggt ggcggcgcgg
    33601 tgttccacga tctcggcaat acctgcttta cctttatggc tggcaagccg gagtacgata
    33661 aaaccatctc cacgtcgatt gtgctcaatg cactgaatgc gctcggcgtc agtgccgaag
    33721 cgtccggacg taacgatctg gtggtgaaaa ccgccgaagg cgaccgcaaa gtctcaggat
    33781 cggcctatcg cgaaaccaaa gatcgtggct tccaccacgg caccttgctg ctcaatgccg
    33841 accttagccg cctggcaaac tatctcaatc cggataaaaa gaaactggcg gcgaaaggca
    33901 ttacttcagt gcgttcccgc gtgaccaacc tcaccgagct gctgccgggg atcccccatg
    33961 agcaggtttg cgaggccata accgaggcct ttttcgccca ttatggcgag cgcgtggaag
    34021 cggaaatcat ctccccggac aaaacgccag acttgccaaa cttcgccgaa acctttgccc
    34081 gccagagtag ctgggagtgg aacttcggtc aggctccggc attctcgcat ctgctggatg
    34141 aacgctttag ctggggcggc gtggaactgc atttcgacgt tgaaaaaggc catatcaccc
    34201 gcgcccaggt gtttaccgac agcctcaacc ccgcgccgct ggaagccctc gccgggcgac
    34261 tgcaaggctg cctgtaccgc gcggatatgc tgcaacaaga gtgcgaagcg ctgttggttg
    34321 acttcccgga ccaggaaaaa gagctacggg agttgtcgac gtggatagcg ggggcggtaa
    34381 ggtaactcgc tgattaaaat atccaaggaa ggaaatatga tcgatctgga aaaccaggaa
    34441 cgggagatta tcaatttaat gttcagccag cgtatttcct ggctagccgc cgtgcgtatc
    34501 aggcataaat tatcgcttgc tgaagtaagt aaaatgttag gtatctcgat taattctcta
    34561 aaacagatcg aaaagacgga acggcttagc tcaaacatca aaagcaaaat ggcagaaatt
    34621 tatggttgtc caccagagtt gctgatatgc ccttcctgga tgacggctga acataaatga
    34681 at
//
LOCUS       DAZKIF010000039        34358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZKIF010000039 DAZKIF010000000
VERSION     DAZKIF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.039"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..124
                     /locus_tag="ACHOTW_001883"
     CDS             <1..124
                     /locus_tag="ACHOTW_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFP9843999.1"
                     /translation="FYESPTSTHNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
     gene            complement(211..886)
                     /locus_tag="ACHOTW_001884"
                     /pseudo
     CDS             complement(211..886)
                     /locus_tag="ACHOTW_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1031..1639)
                     /gene="yiiX"
                     /locus_tag="ACHOTW_001885"
     CDS             complement(1031..1639)
                     /gene="yiiX"
                     /locus_tag="ACHOTW_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP9844000.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1699..2016)
                     /gene="metJ"
                     /locus_tag="ACHOTW_001886"
     CDS             complement(1699..2016)
                     /gene="metJ"
                     /locus_tag="ACHOTW_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP9844001.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2293..3453
                     /gene="metB"
                     /locus_tag="ACHOTW_001887"
     CDS             2293..3453
                     /gene="metB"
                     /locus_tag="ACHOTW_001887"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9844002.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3456..5888
                     /gene="metL"
                     /locus_tag="ACHOTW_001888"
     CDS             3456..5888
                     /gene="metL"
                     /locus_tag="ACHOTW_001888"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP9844003.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6027..6161)
                     /locus_tag="ACHOTW_001889"
     CDS             complement(6027..6161)
                     /locus_tag="ACHOTW_001889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844004.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6237..7127
                     /gene="metF"
                     /locus_tag="ACHOTW_001890"
     CDS             6237..7127
                     /gene="metF"
                     /locus_tag="ACHOTW_001890"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP9844005.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7456..9636
                     /gene="katG"
                     /locus_tag="ACHOTW_001891"
     CDS             7456..9636
                     /gene="katG"
                     /locus_tag="ACHOTW_001891"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP9844006.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9730..10635
                     /gene="yijE"
                     /locus_tag="ACHOTW_001892"
     CDS             9730..10635
                     /gene="yijE"
                     /locus_tag="ACHOTW_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP9844007.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10662..11279)
                     /gene="yijF"
                     /locus_tag="ACHOTW_001893"
     CDS             complement(10662..11279)
                     /gene="yijF"
                     /locus_tag="ACHOTW_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP9844008.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11553..12656)
                     /gene="gldA"
                     /locus_tag="ACHOTW_001894"
     CDS             complement(11553..12656)
                     /gene="gldA"
                     /locus_tag="ACHOTW_001894"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP9844009.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12667..13329)
                     /gene="fsa"
                     /locus_tag="ACHOTW_001895"
     CDS             complement(12667..13329)
                     /gene="fsa"
                     /locus_tag="ACHOTW_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9844010.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13341..15842)
                     /gene="ptsP"
                     /locus_tag="ACHOTW_001896"
     CDS             complement(13341..15842)
                     /gene="ptsP"
                     /locus_tag="ACHOTW_001896"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9844011.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16151..17230
                     /gene="frwC"
                     /locus_tag="ACHOTW_001897"
                     /pseudo
     CDS             16151..17230
                     /gene="frwC"
                     /locus_tag="ACHOTW_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
     gene            17245..17565
                     /gene="frwB"
                     /locus_tag="ACHOTW_001898"
     CDS             17245..17565
                     /gene="frwB"
                     /locus_tag="ACHOTW_001898"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9844012.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17616..19913
                     /gene="pflD"
                     /locus_tag="ACHOTW_001899"
     CDS             17616..19913
                     /gene="pflD"
                     /locus_tag="ACHOTW_001899"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9844013.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19879..20757
                     /gene="pflC"
                     /locus_tag="ACHOTW_001900"
     CDS             19879..20757
                     /gene="pflC"
                     /locus_tag="ACHOTW_001900"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP9844014.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20759..21100
                     /gene="frwD"
                     /locus_tag="ACHOTW_001901"
     CDS             20759..21100
                     /gene="frwD"
                     /locus_tag="ACHOTW_001901"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9844015.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21087..21938)
                     /gene="yijO"
                     /locus_tag="ACHOTW_001902"
     CDS             complement(21087..21938)
                     /gene="yijO"
                     /locus_tag="ACHOTW_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9844016.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22153..23886)
                     /gene="eptC"
                     /locus_tag="ACHOTW_001903"
     CDS             complement(22153..23886)
                     /gene="eptC"
                     /locus_tag="ACHOTW_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9844017.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24069..26720)
                     /gene="ppc"
                     /locus_tag="ACHOTW_001904"
     CDS             complement(24069..26720)
                     /gene="ppc"
                     /locus_tag="ACHOTW_001904"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP9844018.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27072..28223)
                     /gene="argE"
                     /locus_tag="ACHOTW_001905"
     CDS             complement(27072..28223)
                     /gene="argE"
                     /locus_tag="ACHOTW_001905"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP9844019.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28377..29381
                     /gene="argC"
                     /locus_tag="ACHOTW_001906"
     CDS             28377..29381
                     /gene="argC"
                     /locus_tag="ACHOTW_001906"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP9844020.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29389..30165
                     /gene="argB"
                     /locus_tag="ACHOTW_001907"
     CDS             29389..30165
                     /gene="argB"
                     /locus_tag="ACHOTW_001907"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP9844021.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30226..31599
                     /gene="argH"
                     /locus_tag="ACHOTW_001908"
     CDS             30226..31599
                     /gene="argH"
                     /locus_tag="ACHOTW_001908"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP9844022.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVARAIA
                     FARARLG"
     gene            31866..32783
                     /gene="oxyR"
                     /locus_tag="ACHOTW_001909"
     CDS             31866..32783
                     /gene="oxyR"
                     /locus_tag="ACHOTW_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP9844023.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32766..34166)
                     /gene="sthA"
                     /locus_tag="ACHOTW_001910"
     CDS             complement(32766..34166)
                     /gene="sthA"
                     /locus_tag="ACHOTW_001910"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP9844024.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQLLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         7781 a         8665 c         9583 g         8329 t
ORIGIN      
        1 tttttatgaa agcccaacat caacccacaa tgacataatg gggggcgggg gaatgtcata
       61 tgcctatgaa tatgatgtaa aatataatat atgtcatttg agaaaaaaga gcagaatgag
      121 gtaaaaatct ttggctgtgt attcagtctg gtaatcatcc aaggaaagct catcagaagg
      181 gtgatgactc caattagtag aacatatatt ttcgataaaa gcaccgatga ctttttcatg
      241 aagttctaca gaactcgaga aacagatggt tctgcgggcc agacgtttga ttcgggttct
      301 cagggtcaga ttgttacgct caatgtgctg agtaaaaatc ttaccagtca gatgcttctc
      361 ctttggtaac tctctagcgt aactacccca ttcgtcagtg gtcaacatgc cgatggtaaa
      421 agggtaagca gcgccagcaa ttcacgacag gtttcatcag tccgggggcc aaaagtgtag
      481 gccagaacac caccagtttt cgtgttgtac acgtaccaga gccagtgctg tcgcgcttta
      541 ctgccgacaa agctccattg ctgactaagt ttgcagatga gtgccacatc agcatgagcg
      601 accggggatg acgttattct tcgtggcgag agttttttaa agttcggatg actgtattaa
      661 tgccgatttt cagcgtacgg gcagtatagc gaaccccggc accgttgaaa gccatttcag
      721 cgatttggtc tttaactccc ggtttacggg gctcgtaaca gtaactgagc tgaaataccc
      781 ggtgacagtc acggccgcgg aatcgatcat gtcttttggg gttctgcccg cgacggtaaa
      841 cctgaacgga ctggcaacgg ggacaatgaa ggttaactgt gaccatgaaa catccttaaa
      901 agcgagtatt atatccttaa ttcaactaat tggaggcatc accgtttacc ggagcgacag
      961 cttgcggaaa ggtcgtgcaa ctacaaagca acaaacagac tacggcggga attatttttt
     1021 tcatcatcca ttaccacgaa aacagcggcc attctttggc aaccgtggtg agttgtggcg
     1081 catcgaaaac ggcttgtggt gagaccaccg tttcctcaag cggaatattt ttgccgtagc
     1141 gttctttgag cttcgcctgg accagcgggt tgctgagatc aaactctttc aacttctgct
     1201 gttcgcccac acgcattccc agcgcattct gataaacctt ccacaccact tccgaacagt
     1261 actggcgatc gtcgctccat gagaagctga aatcgtacgg tttgccgaga taacgtttcg
     1321 ccgtttgcgc cagtttttgc tgttgttcaa cactcagtcc gccttcaacg cggcgaacaa
     1381 cgtatttgcc cttttcacca tgggcaatcc actgcttgag cggggtgtat ttcaccgggc
     1441 cgaccgcttc aaaaacgtag ggctttttgt tacgcatcac cagcataccg gtgtggctgt
     1501 aatcggaatg ggtcgccagt tggatcgctt tactttgcga tgagcgagag atttgaaaga
     1561 tgatgtcgcc agtttgcggc tgccaggcaa aggcaggcac agaaaccagc aggctgagga
     1621 tcagtagcct gattttcatc gttaaacgtc cttgtatgaa aaaagcggct ggttaactcc
     1681 gcgccgctct tttccgcttt agtattccca cgtctccggg ttaatcccca tctcacgcat
     1741 gatctctttt gccgcttccg ggatttcgtc gctgcgctct ttacgcagat cggcatcatc
     1801 cggcaaaggt tgcccggtaa aggcatgcag aaacgcttcg cacagcagct cgctgttggt
     1861 agcgtgacgc aggttgttca cctgacgacg cgtgcgttca tcggtgagga tttttaacac
     1921 cttaagagga atggaaaccg taatcttttt gacttgttca ctcttcttgc cgtgctcagc
     1981 gtatgggctg atatattcgc cgctccattc agccatgaga tacttaatcc tcttcgtcaa
     2041 taaattgaga ccagaccaca caatttatgt gggtactgac cgtaaacccg catagtttac
     2101 cgtacaggcg ttaccgtgac atcgtgtaat gcacctgtcg gcgtgataat gcatataatt
     2161 ttaacggcta tttgggattt gctcaatcta tacgcaaaga agtttagatg tccagatgta
     2221 ttgacgtcca ttaacacagt gtttactctg gtgcctgaca tttcaccgac caagcccagg
     2281 gaacttcatc acatgacgcg taaacaggcc accatcgcag tgcgtagcgg gttaaatgac
     2341 gacgaacagt atggttgcgt tgtccctccc atccatcttt ccagcaccta taactttacc
     2401 ggatttaatg aaccgcgcgc gcatgattac tcgcgtcgcg gcaacccaac gcgcgatgtg
     2461 gttcagcgtg cgctggcaga actggaaggc ggtgctggtg cagtacttac taataccggc
     2521 atgtccgcga ttcatctggt aacgaccgtc tttttgaaac ctggcgatct gctggttgcg
     2581 ccgcacgact gctacggcgg tagctatcgc ctgttcgaca gcctggcgaa acgcggttgc
     2641 tatcgcgtgt tgtttgttga tcaaggcgat gaacaggcat tacgggcagc gctggcagaa
     2701 aaccccaaac tggtactggt agaaagccca agtaatccat tgttacgcgt cgtggatatt
     2761 gcgaaaatct gccatctggc aagggaagtc ggggcggtga gcgtggtgga taacaccttc
     2821 ttaagcccgg cattacaaaa tccgctggca ttaggtgccg atctggtgtt gcattcatgt
     2881 acgaaatatc tgaacggtca ctctgacgtg gtggctggcg tggtgattgc taaagacccg
     2941 gacgttgtca ctgaacttgc ctggtgggca aacaatattg gcgtgacggg cggcgcgttt
     3001 gacagctatc tgctgctacg cgggttgcga acgctggtgc cgcgtatgga gctggcgcag
     3061 cgcaacgcga aggcgattgt gaaatatctg caaacccagc cgttggtgaa aaaactgtat
     3121 cacccgtcgc tgccggaaaa tcaggggcat gaaattgccg cgcgtcagca aaaaggcttt
     3181 ggcgcaatgt tgagttttga actggatggc gatgagcaga cgctgcgccg tttcctgggc
     3241 gggctgtcgt tatttacgct ggcggaatca ttagggggag tggaaagttt aatctctcac
     3301 gccgcaacca tgacacatgc aggcatggca ccagaagcgc gtgctgccgc cgggatctcc
     3361 gagacgctgc tgcgtatctc caccggtatt gaagatggcg aagatttaat tgccgacctg
     3421 gaaaatggct tccgggctgc aaacaagggg taaaaatgag tgtgattgcg caggcagggg
     3481 cgaaaggtcg tcagctgcat aaatttggtg gcagtagtct ggctgatgtg aagtgttatt
     3541 tgcgtgtcgc gggcattatg gcggagtact ctcagcctga cgatatgatg gtggtttccg
     3601 ccgctggtag caccactaac cagttgatta actggttgaa actaagccag accgatcgtc
     3661 tctctgcgca tcaggttcaa caaacgctgc gtcgctatca gtgcgatctg attagcggtc
     3721 tgctacccgc cgaagaagcc gatagcctca ttagcgcttt tgtcagcgat cttgagcgcc
     3781 tggcggcgct gctcgacagc ggtattaacg acgcagtgta tgcggaagtg gtgggccacg
     3841 gcgaagtgtg gtcggcgcgc ctgatgtctg cggtgcttaa tcaacaaggg ctgccagcgg
     3901 cctggcttga tgcccgcgag tttttacgcg ctgaacgcgc tgcgcaaccg caggttgatg
     3961 aagggctttc ttacccgttg ctgcaacagc tactggtaca acatccgggt aaacgtctgg
     4021 tggtgaccgg gtttatcagc cgtaacaacg ccggtgaaac ggtgctgctg gggcgtaacg
     4081 gttccgacta ttccgcgaca caaatcggtg cgctggcggg tgtttctcgt gtaaccatct
     4141 ggagcgacgt cgccggggta tacagtgccg atccgcgtaa agtgaaagat gcctgtctgc
     4201 tgccattgct gcgtctggat gaagccagcg aactggcgcg tctggcggct cccgttcttc
     4261 acgcccgtac tttacagcca gtatccggta gcgaaatcga cctgcagctg cgctgtagct
     4321 acacgccgga tcaaggctcc acgcgcattg aacgcgtgct ggcctccggt actggtgcgc
     4381 gtattgtcac cagccacgat gatgtctgct tgattgagtt tcaggtgccc accagccagg
     4441 atttcaaact ggcgcataaa gagatcgacc agatcctgaa acgtgctcag gtgcgcccac
     4501 tggcggttgg cgtgcataac gatcgccagt tgctgcaatt ttgctacacc tcagaagtgg
     4561 ccgacagtgc gctgaaaatc ctcgacgaag cgggattacc tggcgaactg cgcctgcgtc
     4621 aggggctggc gctggtggcg atggtcgggg cgggtgtcac ccgtaacccg ctgcattgcc
     4681 accgcttctg gcagcaactg aaaggccagc cggtcgagtt tacctggcag tccgatgacg
     4741 gcatcagcct ggtggcagta ctgcgcaccg gcccgaccga aagcctgatt cagggcctgc
     4801 atcagtccgt cttccgcgca gaaaaacgca tcggcctggt attgttcggc aaaggcaata
     4861 tcggttcccg ctggctggaa ctgttcgccc gcgagcagag cacgctttcg gcgcgtaccg
     4921 gctttgaatt tgtgctggca ggcgtggtgg acagccgccg cagtctgttg agttatgacg
     4981 ggctggacgc cagccgcgcg ttagccttct tcaacgatga agcggttgag caggatgaag
     5041 agtcgttgtt cctgtggatg cgcgcccatc cgtatgatga tttagtggtg ctggacgtta
     5101 ccgccagcca gcagctggct gaccagtatc ttgatttcgc cagccacggt ttccacgtca
     5161 tcagtgccaa caaactggcg ggagcaagcg acagcaataa atatcgccag atccacgacg
     5221 ccttcgaaaa aaccgggcgt cactggctat acaacgccac cgtcggtgcg ggcttgccga
     5281 tcaaccatac cgtgcgcgat ctgatcgaca gcggcgatac tattttgtcg atcagtggga
     5341 tcttctccgg cacgctctcc tggctgttcc tgcaattcga cggtagcgtg ccgtttaccg
     5401 agctggtgga tcaggcgtgg cagcagggct taaccgagcc tgatccgcgt gacgacctct
     5461 ccggcaaaga cgtaatgcgc aagctggtga ttctggcgcg tgaagcgggt tacaacattg
     5521 aaccggacca ggtacgtgtg gaatcgctgg tgcctgctca ttgcgaaggc ggcagcatcg
     5581 accatttctt tgaaaatggc gatgaactta acgagcagat ggtgcaacgg ctggaagcgg
     5641 cccgcgaaat ggggctggtg ctgcgctacg tggcgcgttt cgatgccaac ggcaaagcgc
     5701 gtgtgggcgt ggaggcggtg cgtgaagacc atccgctggc atcactgcta ccgtgcgata
     5761 acgtctttgc catcgaaagc cgctggtatc gcgacaaccc gctggtgatc cgtgggcctg
     5821 gcgcggggcg cgacgtcacc gccggggcga ttcagtcgga tatcaaccgg ctggcacagt
     5881 tgttgtaatt tcagaaattt aataatgccc ggtactcatg ttttcgggtt tatggtttct
     5941 aatgaaatat attgaattat cataggatta ggccggatga agcgtttacg acgaatccgg
     6001 caagaagcaa taagtacatg gttagtttat atttgcagtc cggtttgctt tgcataccgg
     6061 attttctttt tcttaccatc ctgaagtttt ttcatcttcc ctgatttttc ctcaccatca
     6121 ttggtcattt ttcggttgac gcccttcggc ttttccttca tctttacatc tggacgtcta
     6181 aacggatgga tgtgcacaac acaacatata actacaagcg attgatgagg taaggtatga
     6241 acttttttca cgccagccag cgggatgccc tgaatcagag cctggcagaa gtccaggggc
     6301 agattaacgt ttcgttcgag tttttcccgc cgcgtaccag tgaaatggag cagaccctgt
     6361 ggaactccat cgatcgcctt agcagcctga aaccgaagtt tgtatcggtg acctatggcg
     6421 cgaactccgg cgagcgcgac cgtacgcaca gcattattaa aggcattaaa gatcgcactg
     6481 gtctggaagc ggcaccgcat cttacttgca ttgatgcgac gcccgacgag ttgcgcacca
     6541 ttgcacgcga ctactggaat aacggtattc gtcatatcgt ggcgctgcgt ggcgatctgc
     6601 cgccgggaag tggtaagcca gaaatgtatg cttctgacct ggtgacgcta ttaaaagaag
     6661 tggcagattt cgatatctcc gtggcggcgt atccggaagt tcacccggaa gcaaaaagcg
     6721 cccaggcgga tttgcttaac ctgaaacgca aagtcgatgc aggagccaac cgcgcgatta
     6781 ctcagttctt ctttgatgtc gaaagctacc tgcgttttcg tgaccgctgt gtgtcggcgg
     6841 gcattgatgt ggaaatcatt ccgggcatat tgccggtatc taactttaaa caggcgaaga
     6901 aatttgccga catgaccaac gtgcgtattc cggcgtggat ggcgcaaatg tttgacgggc
     6961 tggatgacga tgccgaaacc cgcaagctgg tgggcgcgaa tattgccatg gatatggtga
     7021 agattttaag ccgtgaagga gtgaaagatt tccacttcta tacgcttaac cgcgctgaaa
     7081 tgagttacgc gatttgccat acgctggggg ttcgacctgg tttataaata gtgtggcttt
     7141 tatgaaaatc acacagtgat cacaaatttt aaacagagca caaaatgctg cctcgaaatg
     7201 agggcgggaa aataaggtta tcagccttgt tttctccctc attacttgaa ggatatgaag
     7261 ctaaaaccct tttttataaa gcatttgtcc gaattcggac ataatcaaaa aagcttaatt
     7321 acgatcaatt tgatctacat ctctttaacc aacaatatgt aagatctcaa ctatcgcatc
     7381 cgtggattaa ttcaattata acttctctct aacgctgtgt atcgtaacgg taacactgta
     7441 gaggggagca cattgatgag cacgtcagac gatatccata acacgacagc cactggcaaa
     7501 tgcccgttcc atcagggcgg tcacgaccag agcgcggggg cgggcacaac cactcgcgac
     7561 tggtggccaa atcaactccg tgttgacctg ttaaaccaac attctaatcg ttctaacccg
     7621 ctgggtgagg actttgacta ccgcaaagaa ttcagcaaat tagattacta cggcctgaaa
     7681 aaagatctga aagccctgct gacagaatct cagccgtggt ggccagccga ctggggcagc
     7741 tacgccggtc tgtttattcg tatggcctgg cacggcgcgg ggacttaccg atcaatcgat
     7801 ggacgcggtg gcgcgggtcg tggtcagcaa cgttttgcac cgctgaactc ctggccggat
     7861 aacgtaagcc tcgataaagc gcgtcgcttg ttgtggccaa tcaaacagaa atatggtcag
     7921 aaaatctcct gggccgacct gtttatcctc gcgggtaacg tggcgctgga aaactccggc
     7981 ttccgtacct tcggttttgg tgccggtcgt gaagacgtct gggaaccgga tctggatgtt
     8041 aactggggtg atgaaaaagc ctggctgact caccgtcatc cggaagcgct ggcgaaagca
     8101 ccgctgggtg caaccgagat gggtctgatt tacgttaacc cggaaggccc ggatcacagc
     8161 ggcgaaccgc tttctgcggc agcagctatc cgcgcgacct tcggcaacat gggtatgaac
     8221 gacgaagaaa ccgtggcgct gattgcgggt ggtcatacgc tgggtaaaac ccacggtgct
     8281 ggtccgacat caaatgtagg tcctgatcca gaagctgcgc cgattgaaga acaaggttta
     8341 ggttgggcga gcacttacgg cagcggcgtt ggcgcagatg ccattacctc tggtctggaa
     8401 gtggtctgga cccagacgcc gacccagtgg agcaactatt tcttcgagaa cctgttcaag
     8461 tatgagtggg tacagacccg cagtccggct ggcgcaatcc agttcgaagc ggtagacgca
     8521 ccggaaatta tcccggatcc gttcgatccg tcgaagaaac gtaaaccgac gatgctggtg
     8581 accgacctga cgctgcgttt tgatcctgag ttcgagaaga tctctcgtcg tttcctcaac
     8641 gatccgcagg cgttcaacga agcctttgcc cgtgcctggt tcaaactgac gcacagggat
     8701 atggggccga aatctcgtta catcgggccg gaagtaccga aagaagatct gatctggcaa
     8761 gatccgctgc cgcagccgat ctacaacccg accgagcagg acattatcga tctgaaattc
     8821 gcgattgcgg attctggtct gtctgttagt gagctggtat cggtggcctg ggcttctgct
     8881 tctaccttcc gtggtggcga caaacgcggt ggtgccaacg gtgcgcgtct ggcattaatg
     8941 ccgcagcgcg actgggatgt gaacgccgca gccgttcgtg ctctgcctgt tctggagaaa
     9001 attcagaaag agtctggtaa agcctcgctg gcggatatca tcgtgctggc aggtgtggtt
     9061 ggcgttgaga aagccgcaag cgccgctggt ttgagcattc atgtaccgtt tgcgccgggt
     9121 cgcgttgatg cgcgtcagga tcagactgac attgagatgt tcgaactgct ggagccaatt
     9181 gctgacggtt tccgtaacta tcgcgctcgt ctggatgttt ccaccaccga gtcactgctg
     9241 attgataaag cccagcaact gaccctgacc gcgccggaaa tgaccgcgct ggtgggcggg
     9301 atgcgtgtac tgggtgccaa cttcgatggc agcaaaaacg gcgtcttcac tgaccgcgtt
     9361 ggcgtattga gcaatgactt cttcgtgaac ttgctggata tgcgttacga gtggaaagcg
     9421 accgacgaat cgaaagagct gttcgaaggc cgtgaccgcg aaaccggcga agtgaaatac
     9481 actgccagcc gtgcggatct ggtatttggt tctaactctg tcctgcgtgc ggtggctgaa
     9541 gtttacgcca gcagcgatgc tcacgagaag tttgttaaag acttcgtggc ggcatgggtg
     9601 aaagtgatga acctcgaccg tttcgacctg ctgtaatctg accttcttca gcgactgcct
     9661 ttcaggcagt cgctgaagtt tctttaccgg cgtatagtgt ccacaggaaa actacacact
     9721 ggatctctca tgtctgccgc aggaaagagc aacccactgg caatcagtgg cctggttgtg
     9781 ctcacactta tctggagtta tagctggatt ttcatgaagc aagtcaccag ctacatcggt
     9841 gccttcgact ttaccgcctt acgctgcatt ttcggcgctc tcgttttatt catcgtcctt
     9901 ttattacgtg gtcgcggaat gcgcccgaca ccgtttaaat acaccttagc cattgccctg
     9961 ttacaaacct gcgggatggt tggcctggcg cagtgggcgc tggtcagcgg cggagcgggg
    10021 aaggtggcga tcctgagcta taccatgccg ttctgggtgg tgattttcgc cgcggtgttt
    10081 ctcggtgaac gcctgcgacg tgggcaatat ttcgcgattc tgattgccgc tttcggctta
    10141 tttttggtgt tgcagccgtg gcaactcgat ttctcttcga tgaaaagtgc catgctggca
    10201 atcctctccg gtgttagttg gggggcgagc gcgattgttg ctaaacgtct gtatgcccgt
    10261 catccgcgcg tggatttatt gtcgttaaca tcctggcaga tgctctatgc ggcgctggtg
    10321 atgagtgtgg tcgctttact ggtgccgcaa cgtgaaattg actggcagcc cactgtgttc
    10381 tgggctctgg cctacagtgc gattctggcg acggcgttgg cgtggagctt atggctgttt
    10441 gtgctgaaaa acttgcctgc cagtattgcc agcttaagca cactggccgt tcccgtttgc
    10501 ggcgtactct tttcctggtg gctgctcggt gagaatccgg gggccgttga aggtagcggt
    10561 attgtgctga ttgtgctggc actggcgctg gtgagccgta agaaaaaaga agccgttagt
    10621 gtaaaaagga tctgaatttt ttcttcatgt ggggcgatcg cttatttaac aaaataacga
    10681 taatgcccca ccatgcgcca gctaaacagc acatcttctt cctgcgcacc tgcgccaatg
    10741 ttatgtatca ccagcggcgt accgtcgcgg gcgaagccat ctgaaaccac cccaatatgt
    10801 gccagcccgt tatctaatcg ccaggagaca atatcgcccg cttgatagtc actggggttc
    10861 ttgctgatgg ggcgtgtttt atcgtggcgg gtaaaccagg tttccagatt aggcacccga
    10921 cggtgatcga tgttgctgtc cgggcgcttt aactgccatt tttgcgggta ctcggcaaaa
    10981 ttcttcgcca tatcttcgtg caccagtttc tgcaaatcga ccttctggct gcgcaatgca
    11041 cggatcacca catcggaaca taccccgcgt tcttgcggga catcaccgcc aggataggta
    11101 agctgcacat acgccggatc gtaaaatagc gtgctgccga tttgctgtct ggcaccgtct
    11161 gcgatggcaa ggttggtatt ggcctggatt tgtaccacgg ttggtggaat ggcgggagat
    11221 tttaaggagt agctggtaaa tgccgttagc aggctgacca gcgccagtga agctttcatc
    11281 tgtttcgtcc gtgaaaataa gagtcacaga ttcgaccttc cgggctaacg attgttctgt
    11341 agagaaaatg tccagaagcg gataaaaaat tcctgaacaa cgtgggagtt tcagattact
    11401 ggcacagagt gccggatgcg gcgtgaacgt cttatctggc ctaaaaagca cgcaaattca
    11461 atatattgca gagattgcgt aggcctgata agcgtagcgc atcaggcaat tttgcgttca
    11521 aactcccgga caagccggga gtttggagta ggttattccc actcttgcag gaaacgctga
    11581 ccgtactggt cggctaccag cagagcggcg taaacctgat ctggcgtcgc gccgccaggc
    11641 atgttgtgga tggtttcacc ttctgcacat gccgcttctg ccacaattcg cattttcgcc
    11701 gggacatctt ctttaatatc cagttgagcg agagttattg gcaaacctac cgcatggcta
    11761 agcgcagcta cggtttcgat ttcctcaacc ggcgagtttt ccagtaccag ctgcgtcagt
    11821 gtaccaaatg ccactttttc accgtgataa tagtgatggg catcaggaat ggcagtcagg
    11881 ccattatgca ccgcgtgcgc tgccgccaga ccaccgcttt caaagccaac accgcttaaa
    11941 taagtgttcg cttcaatcac gcgttccagt gccggagtca ctacatgctg ttcggcggcg
    12001 agcatcgctt tttcgccttc ttccagcaag gtgttgtagc acagttcagc cagcgccagc
    12061 gccgcctgag tgcacttgcc gcccgccatg gtggtcgcac cgctacgaga gcaggcacgc
    12121 gcttcaaacc aggtcgccag cgcatcgccg atacccgccg ctaacagacg cgcaggtgcg
    12181 cccgcgacga ttttagtatc gacaatcacc atattcgggt tatttggcag cagcagatag
    12241 cggtcaaatt caccttcgtc agtgtagata acggaaagag cgctacaagg tgcgtcggtt
    12301 gaagcaatgg tcggggcaat ggcgaccgga acacccatga aatgtgccag tgctttggct
    12361 gtatcgaggg tttttccgcc accgataccg agaattgcgc cacactgcgc agtctccgcg
    12421 atgccacgca gacggtcgat ctcattttgc gaacattcac cgccaaacgg cgcaatctct
    12481 actaccagtc cagcatcttt aaagcttttc tcgacagtgg attgagcaaa acctaaaaca
    12541 aatttgtcac ccaccactaa ccagcgttct gccagcggct tcaggtattc gcccagacga
    12601 ttaatcacat cagcgccctg gatgtattta cccggtgatt gaataatgcg gtccataatt
    12661 gctcctttag agatgagtag tgccaaatgc ggcattccag tcgtgttcga acttctctat
    12721 agctgactct accgcagggg tgttgagcat ttgttgcgct acatctaagg gcagggtgat
    12781 ggattcacat cctgccagta aacagtccag cgcctgacgc ggcgttttaa agctggccgc
    12841 cagcaccatg ctttctggtg cgtgcatttc taacagcgct tgcagctcct gaaccgtacg
    12901 aatgccgtcc ccgccctggg catccacgcg gttaacatac ggagcaacgt attttgcccc
    12961 tgccagtgcg gctaataacc cttgtgcggc gctatataca gcagtgccaa gtgtagtaat
    13021 accctctttt ttcagtattt taattgctgc cagaccttcg gaagtcaccg ggattttcac
    13081 cacaatgccc ggaatggcat cgcgcagacg cttcgcttct tccaccatcc cctgcgcgtc
    13141 gcggctcatg gtctgagcaa acagaatgcc ctcatcacca atcgcttttt gcagacgcgg
    13201 cagcacttcc catatggact ccttgctggc agcgataatg ctcgggttag ttgtcacccc
    13261 ggcaatcggg aatatgtgtg ccagacgttc gacttctgcg acgttagcgg tgtccagata
    13321 cagttccatg atgtttcctt ttacagttcc agttcatgtt gcagcaggct ggcgatagcg
    13381 tcggcagagg cggcgttaac cagcgcgtta cggaattctt cgtgcatgat gcggcgagcg
    13441 aggcgcgaga aaatgcgcat atgctgatcg cccgcagcgt gtttgttcag ggttaacata
    13501 atgatgaatt gcgcttcatc atcgccccag cgcaccggcg cttgcagacg cgctacgctg
    13561 atggtggatt gctcaatgtg ttctgatttg ctgtggggaa tggcaaaact aaagcccaga
    13621 ccggtagaga aaacggcctc gcgcgcccac aagtcggctt ccagtttacg cggatagcgg
    13681 cagcggcccg ccagcagcag gttatcggtc atgcctttga gcacttcttc tttgctgcgc
    13741 cagtcgcttt ccagtgtgat gcactcggcg gtgaccagcg gtgcgtcttg ttgggtcatg
    13801 cggaattgcg ccagcaggtg ttccacttcc agcgaagtac ggcaggccat tgcctggttg
    13861 agcaacttgc ggcactcacg gctatcaagt tgcgccatcc gcgctttcgc cgccggaatt
    13921 gatggtgcgc tcatgctgag ttcatccagc cctaagccga ccagcaacgg cagcacggaa
    13981 cctttcgctc ccagctcacc gcacagacca atccatttgc cctggcgatg caccgcttgc
    14041 acggcgtaat cgagcgcccg caagaatgcc ggattcaggc tgttgtagtg acgagtaacc
    14101 ttagcgttat cgcgatccac tgccagcaga tattgcgtca ggtcattact accaatacta
    14161 aagaaatcaa tctcttcgca gcattgatcg atgatgaaca tcaccgacgg cacttccagc
    14221 atgatgccga gctggatctt ctcatcaaac ggaatgtgtt cgttacgcag ttgctgtttg
    14281 gcttccgcca gtttttcttt cacccataag atctcttcca ttgaggagat catcgggatc
    14341 atgattttca ggctgccgtg agcagaggca cggaggatcg accgtagttg tgtggtgaac
    14401 agcgaggcgt attcttcata aatacgcacc gcgcgataac cgaggaacgg gttagcttct
    14461 gccggaatgt tcagataatc aacgggtttg tcaccgccaa tatccatagt gcgcacaata
    14521 atgctgcgtc cgttggcgga ctccagcgct tgacaaaaaa tgttgtacaa ctcgctttcg
    14581 cccggtgcgc tggtgcgatc catatagagc atttcagtgc ggaacaaacc gacgccttcc
    14641 gcaccattgc cgaatgcggc ctgcgcttcc acggagtgag cgatgttagc ggcaatttca
    14701 atgcggatac cgtcagcggt acgggcttgt tgggtcagcc agacacgctg ttgctcacgc
    14761 agggcgtcct gtacgcgggc ttcttgctga taataacgag ctacggcttc ccctggctca
    14821 accacaatcg ccccggcgtt gccgtcgata taaatcgttt gatgctgcca cggagtaagg
    14881 gcatcaatat ctacaccaac cagtgtcgga atgttgaacg aacgggcaag aattacggta
    14941 tgtgaggtgg tgccgccact tttcagtaac agacctttga ggtgattttt atccagttcg
    15001 aggaactggc tgggggttag ttcatcagcc atacaaatgg cgggctgcgt cagtttgccc
    15061 ggtgccggga agcgttgctc accgtagatt tgctgaagca gctggaagca gacatcgcgt
    15121 acgtccaggg cacgttcttg caggtagctg ctgctggaac gggaaaactc ttcacaaaag
    15181 tgattcgcac tggcgacaat cgcttcggcg cagcttaatc cggcgctgac gcctgccagt
    15241 aaatgttcgt gcagggaagt atcgccagcc agggatcggt gcgcttccag aatcgcgctg
    15301 gtggcaccgt cgctatccag cagacgaaac tcaatgtttt tcagtaccag cgtcaggccg
    15361 ttttccagtg cggattgctc ggcgtcaacg tctttggctg cgggaagatt acccagcgca
    15421 ttgagatcta aagaagagat cggcgtcaga atgccgcctg cgctaccgct gcacacggtg
    15481 cgggcgcgga taatttgcgg attcagattg gtcagtgaaa ccggcagtgg ttccagttcg
    15541 tcagatttaa cttccgccag cggcgcatcg cagtggggga attcatcgcg cagccattgg
    15601 cttaaacgct ggtgcgcttc ctgttcgtcg gccccggaaa tcagtaactg gcagttatcg
    15661 cccgccagcg tgtcggtgcc aatcagcgca agggcgcttt tggcgttgcc cttgcggtca
    15721 gtgcgcaggt tatgccactc aatttgtgat gaaaaagtat tacacagcgt ttcaacgtgg
    15781 cttgccggac gtgcatgtac gccgttaggt agctcacaaa taaattccac aatcagggcc
    15841 attgcctctc tcccggtacg gttttctgtt tacaggataa gagcgcacgg caacggcctg
    15901 ccatgtgaca aatctgccaa aagctggaca aatgtaatgt aaccgtcaat ttgcgacgcg
    15961 tctcacaaga cgccgttttg cggcatgctt ccggattatc gcaagttatg aggcggatcg
    16021 catttttgta ctgatattac aaaagtccag taaatggcct ttttatccac tgtttgctcc
    16081 gcctgcgatt gcctattgtt tcctccatca aaaatatgga catgggcttt cgcaagcccg
    16141 ggagcagctt atgaatgagt tggtgcagat cctgaaaaat acccgtcagc atttaatgac
    16201 gggcgtttca cacatgattc ccttcgtggt atcgggcggt attttgctgg cggtttccgt
    16261 catgttgtat ggcaaaggcg cagtgccgga tgccgtagcc gatccgaatc tgaaaaaact
    16321 gtttgatatc ggcgttgcag gtttgacgct gatggtgcct ttcctcgccg cttacatcgg
    16381 ttactccatt gcagagcgtt ctgcgctggc tccgtgcgct atcggtgcct gggttggtaa
    16441 cagctttggt gcgggcttct ttggtgcact gatcgccggg attatcggcg gcatcgtggt
    16501 gcattacctg aagaaaattc cggtgcataa agttctgcgc tcggtgatgc ccatcttcat
    16561 cattccgatc gtcggcacac tgattaccgc aggcatcatg atgtggggtc tgggcgagcc
    16621 tgtaggggcg ttgaccaaca gcctgactca gtggcttcag gggatgcagc agggcagcat
    16681 tgttatgctg gcggtgatca tgggtctgat gctggcgttc gatatgggcg gtccggttaa
    16741 caaagtggcc tatgccttca tgctgatttg cgttgctcag ggtgtttata ccgtggtggc
    16801 cattgctgcc gtgggtattt gtatcccgcc gctggggatg gggctggcga cgctgattgg
    16861 tcgtaaaaat ttctccgcag aagagcgcta aaccggtaaa gcggcgctgg tgatggggtg
    16921 cgttggggtt actgaagggg cgattccttt cgccgcagcc gatccgttac gtgtaattcc
    16981 ttccatcatg gttggttcag tttgtggtgc agtaactgcg gcgctggtcg gtgcgcagtg
    17041 ctatgcaggc tggggtggtc tgattgtgct gccggtggtt gaaggtaagt tgggttatat
    17101 cgcagcagtg gctgtcgggg cggttgtgac ggcagtctgc gtgaacgtgc tgaaaagtct
    17161 ggcgcgtaaa aatggatctt ctactgatga aaaagaagac gacctggatt tggattttga
    17221 aattaattaa ttgaggaacg agccatgacg aaaattattg cagtaaccgc gtgtccatct
    17281 ggtgttgccc atacttatat ggctgcagag gcgctggaaa gtgccgcgaa agcgaaaggc
    17341 tgggaagtga aagtagaaac ccagggctcg attggtctgg aaaacgaact gactgcggaa
    17401 gacgtggcga gcgccgatat ggtgattctg actaaagata tcggcatcaa gtttgaagag
    17461 cgttttgctg gcaagaccat cgtgcgcgtc aacatcagcg atgcggtaaa acgtgctgat
    17521 gccattatga gcaaaattga agctcacctg gcgcaaaccg cttaagttct tctcccgctc
    17581 gcaagggcgg gttcgctttc ccacaggagt tcctcatgac gaatcgtatc tctcgcctca
    17641 aaactgcact gtttgccaat acccgtgaaa tctcgctgga gcgtgcgctg ctttataccg
    17701 ccagccatcg gcaaaccgaa ggcgaaccgg tgatattgcg ccgggcgaaa gcaacagcgt
    17761 atatccttga acatgttgaa atttcgattc gtgatgaaga actgattgcc ggtaaccgca
    17821 ccgtaaaacc gcgcgccggg attatgtcgc cggaaatgga cccttactgg ctgctgaaag
    17881 agctgggtca attcccgacg cgtccgcagg accgttttgc tatcagcgaa gaagataaac
    17941 gtatctaccg cgaagagttg ttcccgtact gggaaaaacg ttcgatgaaa gatttcatca
    18001 acgggcagat gacggatgaa gtaaaagccg cgactagcac gcagattttc agcatcaacc
    18061 agacggataa aggccagggg cacattatta ttgattaccc gcgcctgctg aatcacgggc
    18121 tgggggagct ggtggcacag atgcagcaac attgtcagca acagccggag aatcactttt
    18181 atcaggcagc gctgttactg ctcgaagcct cgcagaaaca cattttgcgt tacgccgaac
    18241 tggcggaaac gatggcggca agctgcactg atgcccagcg tcgcgaagaa ctgttgacta
    18301 ttgcggagat ctcccgccat aacgccgaac ataaaccgca gacgttctgg caggcgtgcc
    18361 agttattctg gtacatgaac atcattctgc aatacgaatc caacgccagt tcgctgtctt
    18421 tagggcgctt cgaccagtat atgttgccgt tctatcaggc atctttaacc cagggcgaag
    18481 atccggcgtt cctgaaagaa ctgctcgaat ctttatgggt gaaatgcaac gacattgtgc
    18541 tgttgcgctc caccagcagc gcgcgttatt tcgcaggttt cccgactggc tataccgcac
    18601 tgctcggcgg gttaaccgag aacggacgta gcgcggtgaa cgtgctttcg ttcctctgcc
    18661 tcgatgccta tcaaagcgta caattaccgc aaccgaacct aggggtgcgc actaacgcct
    18721 tgatcgacac gccgttcctg atgaaaaccg cagaaaccat tcgcctcggc accggtattc
    18781 cgcaaatctt taacgatgaa gtggtggtgc cagcgttcct caaccgtggc gtttcgctgg
    18841 aagatgcgcg cgactattcc gtagtgggct gtgtggaatt atctattccc ggcagaacct
    18901 acggcttgca tgacatcgcg atgtttaatc tgctgaaagt gatggaaatc tgcctgcatg
    18961 aaaatgaagg caatgctgcg ctgacttatg aaggtttact ggagcagatc cgcgccaaga
    19021 tcagccacta catcaccctg atggttgagg gcagcaatat ttgtgatatc ggccatcgcg
    19081 actgggcacc tgtaccgctg ctctcgtctt ttatcagcga ttgtctggaa aaaggtcgcg
    19141 atattaccga tggcggcgcg cgttataact tctccggcgt acagggaatt ggtatcgcca
    19201 acctgagcga ttctctccat gcgttgaaag ggatggtttt tgatcaacag cgtttaagtt
    19261 ttgacgaatt gctgtcggta ttaaaagcca actttgcaac gccagaaggc gaaaaagtcc
    19321 gcgctcgctt aattaaccgc tttgagaaat acggtaacga tatcgacgag gtgggtaaca
    19381 ttagcgccga actgttgcgc cactactgca aagaagtgga aaaataccag aacccgcgcg
    19441 gcggttactt cacgccggga tcgtataccg tttctgctca cgtcccgttg ggatcggtgg
    19501 ttggcgcgac gccagacggt cgttttgccg gagaacagtt ggcagacggc ggcttgtcac
    19561 ccatgctggg ccaggacgca caagggccaa cagcggtact gaagtcagtc agtaagctcg
    19621 ataacacgct gctgtctaac ggtacgttgc tgaacgtgaa attcactccg gcgaccctgg
    19681 aaggtgaagc gggattacgc aaactggccg acttcttacg ggcgtttacc cagcttaagt
    19741 tgcagcatat tcagtttaac gtggtgaacg ccgacacgtt gcgggaagcg caacagcgcc
    19801 cacaagatta tgccgggctg gtggtgcgcg ttgccggata cagcgccttc tttgtcgaac
    19861 tgtcgaagga gatccaggat gacatcatcc gccggacagc gcatcagctg taacgttgtg
    19921 gaaacgcgcc gcaatgatgt ggcgcgcatt ttcaacattc agcgttattc actgaatgac
    19981 ggcgagggca ttcgtacggt ggtctttttt aaaggctgtc cgcatctttg cccgtggtgt
    20041 gctaatccgg agtcgatctc cggcaaaatc cagacggtac gcagagaggc gaaatgtctg
    20101 cactgtgcga aatgtttgcg tgatgcggat gaatgcccct ccggggcgtt tgaacggatt
    20161 ggtcgcgata tcagccttga cgcgctggaa cgggaagtga tgaaagatga cattttcttt
    20221 cgcacgtccg gcggcggcgt cacgctttct ggcggcgaag tgttaatgca ggcggagttt
    20281 gctacccgtt ttttacagcg gctgcggttg tggggcgtgt catgcgccat tgaaactgcc
    20341 ggagacgcgc cagccagcaa gctgttaccg ctggcgaaat tgtgcgatga agtgttgttc
    20401 gatttaaaaa ttatggacgc gactcaggcg cgggatgtgg tgaagatgaa cctgccacgc
    20461 gtgctggaga atctgcgttt gctggtgagt gagggcgtca acgtgatccc gcgtttaccg
    20521 ctgatccctg gtttcacgct cagccgggag aatatgcagc aggcgctgga tgtactgatc
    20581 ccgctgaaaa tcaggcagat ccatctgtta ccgtttcatc agtacggcga accgaaatac
    20641 cgcctgctgg ggaaaacatg gtcgatgaaa gaggtgcctg cgccgtcgtc agccgatgtg
    20701 acaacgatgc gcgaaatggc agaacgggcc ggatttcagg ttaccgtggg aggttaaaat
    20761 ggcatacctg gtggcagtaa ccgcctgcgt aagcggcgtg gcgcatacat atatggcggc
    20821 ggaacggctg gaaaagttgt gcctgttaga gaagtgggga gtcagcattg aaactcaggg
    20881 cgcgctggga acggagaatc gtttagcgga cgaggatatc cgtcgggcgg atgttgctct
    20941 gttgattacg gatatcgagc ttgccggtgc cgagcgattt gaacattgcc gctatgtgca
    21001 atgcagcatc tacgcattcc tgcgtgagcc gcagcaggta atgagcgcgg tgcgcaaagt
    21061 gctttctgcg ccgcagcaaa cccatcttat tctggagtag tcggtttttc tgtcagttgg
    21121 ctgtggtact gccggcgata ttccgacggc gagcgttcgg tgtttttacg aaacagacgg
    21181 cagaagtagt tgctgtcgac aaagccgcag gcgtgcgcca cttcttttac cttcaggtca
    21241 tagcctttca ataacgtctt agcgtgctcc agtcgcgtgt gattcaggta ttcgttaaaa
    21301 ccaatggccc ccgttttttg aaacaggtgc gagagatagt ttggtgaaat atagaatgcc
    21361 tgtgcgacag attcgcgggt aagcgcggag gcatagcgtt cgtcgatata atcgcgaata
    21421 gcttcgaata gtgcctggct gcgtgaggcg gtctggattt ggctgccaag caaatcgcgg
    21481 cagtggctaa gcaggctggc gacgataagc cttgccgttt gctgctcctg cggctgcatt
    21541 tgcatttcat tgagcgtttg tagcagaaaa gaacctatgc gtgggccgcg tcgggcgacg
    21601 tgttgcttcg ccagattttg atattgtttg ccatcccatt gcacgacgct gaagccgagt
    21661 tgctgtttgc caaacagcac gctaaaggta gtagcagggg cttgccattg tggaaaattc
    21721 cagccaccag cggcaacgta cagcacatcg ccgggaacta acgttgctcc agcgccggta
    21781 tcaacaaact caccttccag cacaatttcc agccgtggaa aatcgacctg atacgcaaga
    21841 tcgggcaccg ggccgttact gctggcaaag taaatctggc gcagggacag cgggccgttg
    21901 atcaggcggg agagcagata gctgacgtcg tgatacatgt tattccctta ggtatggaga
    21961 cgggaaaaca gtaactggca ttattaaaaa aggccacctg aaaagtgtgg cctgagggga
    22021 gttcgatgta aacattgcgg cagttgtatt gccggatgtg gcgtaaacgc cttatccagt
    22081 ctaccgccgc gatctgtagg ccggataaga cgcgtcaagc gtcgcatccg gcaaatagcg
    22141 cctggctgat aattactgat tacctacctg gtcgccatac ggcagcgtgt cgtaatcgat
    22201 cagcgcgttt ttcttgtacg ggttaccaat ccagcgggta ttttctttga actgcggatt
    22261 caccactgaa cgggttggat cgtaaccgtc gtaagataag cccgctaaat ctgaccaggt
    22321 gtggatcagt tccgcaaggc tgtatttacg atcaacatcc tgcgagaaat cacgcggatg
    22381 agtcgcttgc catttttccg acgtccacag caggaacgga atggtgtaca tatgacgcgt
    22441 cgggttgtcc tcattacgcc cctgggtttt atgaggcggc gtgtcgtaaa cctcttcacc
    22501 gtggtcagag aagtaaacca ggaaaccgtt cgggtctgct gctttaaagt ctttaatcag
    22561 gctggcaacc acatgatcgt tatacaggtt agcgttgtca taatcgttat atgactccag
    22621 ctcttccgcg tttaatcccg gcggaacatg atcggtattg ccatcaaact tgccctgatt
    22681 ttccgggtag cggtatttgt atttgatatg cgtacccagc agatgaacga tgatcagttt
    22741 cttcggcgca gggtccttca gcacttcctg gaacggcttc agcacgttag tgtcgtattc
    22801 acgcgcactc tgcgtacgtt gctggttcat gtagtactgc ttgtcggtct ggcgcgaaaa
    22861 taccgtcagc atggtattgc gggcggtcat cgtctgctgg ttggtgatcc agaaggtttt
    22921 ataacccgcc tgtttcatca tgttcatcag cgacggctgc gtcagataca gatccgggtt
    22981 cttttcattg gcaaaggtca gcgcctgttg caggatttca atggtgtacg gacgagacgt
    23041 aactacgtta ttaaacacgg tcagattcgg atcggtttta tgcagcgcat tcagctccgg
    23101 cgtggtttca cgcggataac cgtacagact catgcgtcca cgctgggttg actcgccaat
    23161 caccagcact aaggtgcgcg gttcgttacc cgattcatct ttgaaattag ccagcggcgg
    23221 caaggcatta ttttcattca gtagctttgt tagcgagttt agttgctgac gatactgata
    23281 atagccggtc aggaattgcc acggtgcggc aggctccata cgcgaggcca ggttatccaa
    23341 cgttttctca aacggcttgt ttttgataaa catattcatg gcgatcggat gcagaatcaa
    23401 gccataaagc agggcaaaag agacaacata acgccacggc tttggaatat agaccgggcg
    23461 caggcgtgtc cacagcagaa ctgccaccgc cgtataggcc agcgcgataa gcacaatttt
    23521 caggctgaaa tactggctta aatactcgct ggcttcgttg gtgttggttt cgaacatcac
    23581 aaacagaacg ctctgcgaga actcctgacc gtagatgacg tagtagcaca gcgccgccag
    23641 agaggccgcc catagcacca cgccgatcac tgcggcaata attttgatcc gcttcggaaa
    23701 gaggaatacc ggaattaacc acagcgagct gaataacagc gagtcgcgaa tgccgttggt
    23761 gccactataa ccactgatgt aaataatggc ctgtagcaga gtagagaaaa accaaaagta
    23821 gagcaatgcc caacccaggg ctttccagct aaaaagaggt ttagcctgga cttctgtgga
    23881 atgcatagta agaacctgtc ttgaaaaaaa tatcgccgaa tttaccgaca attccttaag
    23941 gatatctgaa gggatatcca gaatttgaat aaaatgcaga cagaaatata ttgaaaacga
    24001 gggtgttaga acagaagtat ttcagaaaac cctcgcgcaa aagcacgagg gtttgcagga
    24061 gaggaagatt agccggtatt acgcatacct gccgcaatcc cggcaatagt gaccattaac
    24121 gcctgctcga cgcgaggatc cggttcctgg ccttcttttt ctgcctggcg ggagcggtgc
    24181 agcaactcgg cttgcaatac gttcagcggg tcggtgtaaa tattccgtag ctgaatagac
    24241 tctgcaatcc acggcagatc ggccatcaga tgggaatcgt tggcaatcgc cagcaccact
    24301 ttgatgtctt cttcttgcag attgcgtaac tctttaccta acggccacag tgttttgtct
    24361 accaggcgtt ggtcatagta ttccgccagc cacaggtctg ctttggcgaa gaccatctcc
    24421 agcatgccga gacgcgtcga gaagaatggc caatcgcggc acatagcctc cagctcgctc
    24481 tgtttgccat cttcgaccac tttttgcagc gccgtacctg cacccagcca ggcggggagc
    24541 atcagacggt tttgcgtcca ggcgaagatc cacggaatgg cgcgtagtga ctcgacgccg
    24601 ccggttgggc gacgtttcgc cggacgtgaa cccaacggca gtttgcccag ttcctgttcc
    24661 ggcgtagcgg agcggaagta aggcacaaaa tctttgtttt cacgtacgta gccacggtag
    24721 agatcgcagg agatgactga tagttcatcc ataatgcgac gccagctctc tttcggctca
    24781 ggtggtggca gcaggttggc ttccagtatc gccccggtgt aaagcgacag gctgctgacg
    24841 gtgatttctg gcagaccata tttaaagcgg atcatctcgc cctgttcagt cacgcgcagg
    24901 ccacctttca ggcttcctgg cggttgtgac agcagcgccg catgagcagg tgcgccgccg
    24961 cgaccaatgg aaccgccgcg accgtggaac aacgtcagct caatacccgc tttttcgcag
    25021 gttttgatta atgcatcctg tgcctgatat tgcgcccagg acgctgccat cacgcccgca
    25081 tcttttgctg agtcggaata gccaatcatc accatctgtt tgccctgaat caggccgcga
    25141 taccagtcga tattcagcaa ctgagtcatg acatcgttgg cgttgttcag gtcatcgaga
    25201 gtttcaaaca gcggagcaac cggcatcgca aacccgatac ccgcttcttt cagcagcagg
    25261 tggacagcca gcacgtcgga cggcgttttc gccatcgaga tcacgtaggc ggcaatggag
    25321 ccttgcggtg cttcggcaat cacctggcag gtatcgagca cttcgcgcgt ttcggcgctt
    25381 ggttgccagt tgcgcggcag aagcggacgt ttggagttca gttcgcggat caggaacgcc
    25441 tgtttgtcgg cctctgacca gctttcgtag tcgccgatac cgaggtagcg ggtcagctcg
    25501 cccagcgctt cggtatgacg cgtgctctcc tgacggatat caatacggac cagcggtacg
    25561 ccgaaacatt tcacgcggcg cagggtgtcg agcagatcgc cgttggcgat aatacccatg
    25621 ccacacgcct gaagtgactg gtagcaagcg tagagcggtt cccacagttc ttcgttttgt
    25681 gtcagcaggc cttctggttt tggcagttct tcgcctttca ggcgcgcttc cagccatgcc
    25741 tgtgtcgcca tcaggcgaga acgcaggttt ttcatcagat agcgatacgg ttctgcggca
    25801 ccttcttcgc caaccagcgc cagcagttca ggggtcgctt caaccatcga cagttcagaa
    25861 accagaacct gaatatcttt caggaacaga tcggtggctt tccagcggct gagcagcagg
    25921 acgtggcggg tgatatcggc agtgacgttc gggttgccgt cgcggtcacc gcccatccac
    25981 gaggtaaaac gtaccggaac aaattcgacg ggcagtttgt agccgaggtt ctcttccagt
    26041 tgttcgttca gttcgcgcag gtaattcggt acgccttgcc acaggctgtt ttctactacg
    26101 gcaaagcccc atttggcttc atctaccggg cttggacgca gcttacggat ttcatcggta
    26161 tgccatgact gggcgatcaa ctggcgcagg cgacgcatca gctggttgtg ttcgtagtca
    26221 gcgatatctt tgttatcgag ctgttttaag caggcgttca cttccaccat tttgtggatc
    26281 agtgtacgac gggtaatttc ggttgggtga gccgtgagga ccagttccag cgacagcgat
    26341 tccactgctt ttttgatggt gtcttcgctc agttccggct ggtttttcag tttacgcagg
    26401 gtgcgggcga tcacttccgg gttgctggca gcttcgcctt tcggcgaaat gctgtggtat
    26461 tgctcggcgg tgttggccag gttcaggaac tgactaaacg cacgcgcaac gggcagcagc
    26521 tcgtcgttcg acaaattttg taaggtggtg agtaactcct ggcggttagc atcattgcca
    26581 gcgcgtgaag atttggacaa cttacggata gtttctacgc gttcaagaat gtgttctccc
    26641 aacgcatcct tgatggtttc tcccagcact ttgccgagca tactgacatt actacgcaat
    26701 gcggaatatt gttcgttcat attaccccag acaccccatc ttatcgtttg atagccctgt
    26761 atccttcacg tcgcattggc gcgaatatgc tcgggctttg cttttcgtcg tcttttataa
    26821 agccacgtaa aagcggtgac gtcaaatgct gcgaaatcgc ttcagcaaac gaataaatag
    26881 caggaattta cgtcattaaa ttcacgacgc tttaaataag cgtaacttat gggaatgtta
    26941 aaaaatcgcc tcaagtaaca ccaaaggtgt aggtcggata agatgtgcaa gcatcgcatc
    27001 cgacattatt gcggcactgg agtttggcaa cagtgccgga tgcggcgcaa gcgccttatt
    27061 cggcctacgt tttaatgcca gcaaaaatgg tgaatgacct gggttatcag ttcgcgggtg
    27121 ggcttgataa accgtgtttc cagatattca tcaggctgat gagcctgatt aattgagcca
    27181 ggccccagaa ccagcgtcgg gcataacgtt tgaataaacg gcgcttcggt acagtaattc
    27241 accacttcgg tttttgctcc gagcaatttc tcaaccactt caaccagttg atgattcggt
    27301 gggcattcat agccagggat cggcggatgc agctcgtcga ccgtcagacg acccggccag
    27361 cgttcgctca ccggagccaa tgcatcgttg agcaaaccat taagttcatt gagtgtcatg
    27421 ccaggcagcg gacgaatatc catatgcaac tcacaccaag cgcaaatacg gttagaagcg
    27481 tcgccaccgt gaatatgccc gaggttgagc gtagggtatg gcacggtaaa cgcttcgtag
    27541 tgataacgtt ctttcaggtt atcgcgcaat tgcaaaatat gcccgatggc gtcgtgcatt
    27601 agttctatag cgttaactcc gcgtgctgga tcgctggagt gccccgactg gccctgaata
    27661 cggatggcgt tagagatatg acctttatgt gcgcgtaccg gttgtagtga cgtcggttcg
    27721 ccaatgatgg cgcaatccgg gcgcagggcg gtagtttcgg caaaataacg cgctccggcc
    27781 atactggttt cttcatcagc agtcgccaga atgtagagcg gttttttcag tttcgtgacg
    27841 tcgacatcgc gtagcgcatc aaggataaac gcaaaaaagc ctttcatgtc ggcggtgcct
    27901 aagccgtaaa gcttgccgtc atgctccgtc agtgtaaacg gatcgcgcgt ccagcgaccg
    27961 tcatcaaatg gcaccgtatc ggtatgcccc gccagcaaca agccgccagc cccctgtccg
    28021 gtacttgcca gcatattaaa tttgttgcga gttcctggta caggctgcac ttccacattg
    28081 aagcctaaat ctttaaacca gtccgccagc agagtgatta aatctgcatt gctttgatcg
    28141 agtgcctctt ccgtggcgct tattgaaggt gtggcaatca gagcacggta aatctcgata
    28201 aatggcggta atttgttttt cattgttgac acacctctgg tcatgatagt atcaatattc
    28261 atgcagtatt tatgaataaa aatacactaa cgttgagcgt aataaaaccc accagccgta
    28321 aggtgaatgt tttacgttta acctggcaac cagacataag aaggtgaata gccccgatgt
    28381 tgaatacgct gattgtgggt gccagcggct acgctggcgc agagctagtg acctgtgtaa
    28441 atcgccatcc gcatatgaac ataaccgctt tgactgtttc agcgcaaagc aatgatgcgg
    28501 gaaagttaat ctccgatttg catccgcagc taaaaggcat cgttgatctg ccgttgcagc
    28561 cgatgtcgga tatcagcgag tttagccctg gggtggacgt ggtgtttctc gccaccgccc
    28621 atgaagttag ccacgattta gcgccgcagt ttcttgacgc gggctgcgtg gtgttcgacc
    28681 tttccggcgc gtttcgtgtt aacgacgcca ccttctatga aaaatattac ggctttaccc
    28741 atcaataccc ggaactgctg gaacaggcag cctacggtct ggcggagtgg tgcggtaata
    28801 aattaaaaga agcgaatttg attgcggtgc caggctgtta tccgacggca gcacagttgg
    28861 cgctgaaacc gttgattgat gccgatcttc ttgacctcaa tcagtggccg gtgatcaacg
    28921 ccaccagcgg cgtgagcggt gcaggacgta aggcagcgat ttcaaatagt ttttgtgaag
    28981 ttagcctgca accgtatggc gtctttaccc atcgccatca accagagatc gccacacacc
    29041 tcggtgctga cgttatcttc accccgcatc tgggcaattt cccgcgcggc attctcgaaa
    29101 ccattacctg ccgcctgaaa ccgggtgtga cccaggcgca agtggcgcaa gcgttacagc
    29161 aggcgtatgc ccataaaccg ctggtgcggc tgtatgacaa aggcgttccg gcgctgaaaa
    29221 atgttgttgg gctaccattt tgcgatatcg ggtttgccgt tcagggcgaa catctgatta
    29281 ttgtggcgac cgaagacaac ttactgaaag gcgcggcggc acaagcggta cagtgcgcca
    29341 atattcgttt cggctatgcg gaaacgcagt ctcttattta agggtgcaat gatgaatcca
    29401 ttaattatca aactgggtgg cgtactgctg gatagtgaag aggcgctgga acgtctgttt
    29461 agcgcactgg tgaattatcg tgagtcacat cagcgtccgc tggtgattgt gcacggcggc
    29521 ggttgcgtgg tggatgagct gatgaaaggg ctgaatctgc cggtgaaaaa gaaaaatggc
    29581 ctgcgggtga cgcctgctga tcagattgac attatcaccg gagcactggc gggaacggca
    29641 aataaaaccc tgttggcatg ggcgaagaaa catcagattg cggccgtagg tttgtttctc
    29701 ggtgacggcg acagcgtcaa agtgacccag cttgatgaag agttaggtca tgttggactg
    29761 gcgcagccag gttcgcctaa gcttatcaac tccttgctgg agaacggtta tctgccggtg
    29821 gtcagctcca ttggcgtaac agacgaaggg caactgatga acgtcaatgc cgaccaggcg
    29881 gcaacggcgc tggcggcaac gctgggcgcg gatctgattt tgctctccga cgtcagtggc
    29941 attctcgacg gcaaagggca acgcattgcc gaaatgaccg ccgcgaaagc agaacaactg
    30001 attgagcagg gcattattac tgacggcatg atagtgaaag tgaacgcggc gctggatgct
    30061 gcgcgcacgt tgggccgtcc ggtagatatc gcctcctggc gtcatgcgga gcagcttccg
    30121 gcactgttta acggtatgcc gatgggtacg cggattttag cttaagtttt gttggccgga
    30181 ggcgcagctt tccggcattg aatttcaaaa taaggaaaca gagttatggc actttggggc
    30241 gggcgtttta cccaggcagc agatcaacgg ttcaaacaat tcaacgactc actgcgcttt
    30301 gattaccgtc tggcggagca ggatattgtt ggctctgtgg cctggtccaa agccctggtc
    30361 acggtcggcg tgttaaccgc agaagagcag gcgcaactgg aagaggcgct gaacgtgctg
    30421 ctggaagatg ttcgcgccag gccacaacaa atccttgaaa gcgacgccga agatatccat
    30481 agctgggtgg aaggcaaact gatcgacaaa gtgggccagt taggcaaaaa gctgcatacc
    30541 gggcgtagtc gtaatgatca ggtagcgact gacctgaaac tgtggtgcaa agataccgtt
    30601 agtgagttac tgacggctaa ccggcagctg caatcggcgc tggtggaaac cgcacaaaac
    30661 aatcaggacg cggtcatgcc aggttacact cacctgcaac gcgcccagcc ggtgacgttc
    30721 gcgcactggt gcctggccta tgttgagatg ctggcgcgtg atgaaagccg tttgcaggat
    30781 gcgcttaagc gtctggatgt cagcccgtta ggttgtggcg cgctggcggg aacggcttat
    30841 gaaatcgacc gtgaacagtt agcgggctgg ctgggatttg cttcagcaac ccgtaacagt
    30901 ctcgacagcg tttctgaccg tgaccacgtg ctggaactgc tttctgctgc cgctatcggc
    30961 atggtgcatc tgtcgcgttt tgctgaagat ctgattttct ttaacaccgg cgaagcgggg
    31021 tttgtggagc tttctgaccg cgtgacttcc ggttcatcat taatgccgca gaagaaaaac
    31081 ccggatgcgc tggagctgat tcgcggtaaa tgcggtcggg tgcagggcgc gttaaccggc
    31141 atgatgatga cgctgaaagg tttgccgctg gcttacaaca aagatatgca ggaagacaaa
    31201 gaaggtctgt tcgacgcgct cgatacctgg ttggactgcc tgcatatggc ggcgctggtg
    31261 ctggacggca ttcaggtgaa acgcccgcgt tgccaggaag cggcgcaaca gggttacgcg
    31321 aacgccactg aactggcgga ttatctggtg gcgaaaggtg tgccgttccg cgaggcacac
    31381 catattgtgg gtgaagctgt ggtggaagcc attcgtcagg gcaaaccgct ggaggagctg
    31441 ccgctcaccg agttgcagaa atttagtccg gtgattggtg aagatgtcta tccgattctg
    31501 tcgctgcaat cgtgcctcga caagcgtgcg gcaaaaggcg gcgtctcacc acagcaggtg
    31561 gcgcgggcga ttgcttttgc aagggcgcgg ttggggtaag aacatttata tgtacaaatt
    31621 tgagcctggc ttatcgccgg gcttttttat ggcaaaaaaa agcggatcct ggagatccgc
    31681 aaaagttcac gttggcttta gttattcgag ttgagaaact ctcgaaacgg gcagtgactt
    31741 caagggttaa aagaggtgcc gctccgtttc tgtgagcaat tatcagtcag aatgcttgat
    31801 agggataatc gttcattgct attctaccta tcgccatgaa ctatcgtggc gatggaggat
    31861 ggataatgaa tattcgtgat cttgagtacc tggtggcatt ggctgaacac cgccattttc
    31921 ggcgtgcggc agattcctgc cacgttagcc agccgacgct tagcgggcaa attcgtaagc
    31981 tggaagatga gctgggcgtg atgttgctgg agcggaccag ccgtaaagtg ttgttcaccc
    32041 aggcgggaat gctgctggtg gatcaggcgc gtaccgtgct gcgtgaggtg aaagtcctta
    32101 aagagatggc aagccagcag ggcgagacga tgtccggacc gctgcatatt ggtttgattc
    32161 ccacggttgg accgtacctg ctaccgcata ttatcccgat gctgcaccag acctttccaa
    32221 agctggaaat gtatctgcat gaagcacaga cccaccagtt actggcgcaa ctggacagcg
    32281 gcaaactcga ttgcgtgatc ctcgcgctgg tgaaagagag cgaagcattc attgaagtgc
    32341 cgttgtttga tgagccaatg ttgctggcta tctatgaaga ccacccgtgg gcgaaccgcg
    32401 aatgcgtacc gatggccgat ctggcagggg aaaaactgct gatgctggaa gatggtcact
    32461 gtttgcgcga tcaggcaatg ggtttttgct ttgaagccgg ggcggatgaa gatacacact
    32521 tccgcgcgac cagcctggag acgctgcgca acatggtggc ggcaggtagc gggatcactt
    32581 tactgccagc gctggctgtg ccgccggagc gcaaacgcga tggggttgtt tatctgccgt
    32641 gcattaagcc ggaaccacgc cgcactattg gcctggttta tcgtcctggc tcaccgctgc
    32701 gcagccgcta tgagcagctg gcagaggcca tccgcgcaag aatggatggc catttcgata
    32761 aagtgttaaa acaggcggtt taaaccgttt aacgcagcta cccgataggc ttccgccatc
    32821 gtcgggtagt taaaggtggt gttgacgaag tactcaatag tgttgccgcc acctttctgt
    32881 tccataatcg cctgaccgat atgaataatt tcggcagcgc gctcgccaaa gcagtgaatg
    32941 cccagaatct cttttgtttc ccgatggaac aaaattttca gcgtgcccac gttcatgccg
    33001 acgatttgtg cacgtgccag atgtttgaac tgggcgcggc ccacttcata tggcactttc
    33061 atcgcggtca gcagctgttc ggttttgccc acagagctga tttccgggat ggtgtaaatg
    33121 ccggtaggga tatcttcaat cagatgcgcg gtggcttcgc cttttaccag cgcctgcgcg
    33181 gcaatgcgcc cctggtcata ggccgctgat gccaggctcg gataaccaat cacgtcgccc
    33241 accgcgtaaa cgtgcggctg tgcggtctga tacatgctgt tgaccttcag ctgtccgcga
    33301 ctgtcagttt ccagcccaat gttctgtaac gccagcgaat cggtattacc ggtacgaccg
    33361 ttggcataga gcaggcagtc agctttcagt tttttacccg acttcagatg catgatcaca
    33421 ccgtcgtcac agccttcgat cttctcgtac tcttcgttgt gacgaatcac tacgccactg
    33481 ttccagaagt gataggagag agaatctgac atctcttgat cgagaaatgc cagcaggcga
    33541 tcgcgggtgt tgatcagatc cacttttaca tccataccgc ggaagatcga cgcatattca
    33601 cagccgatca ctccagcacc atagataagt acatggcgcg gttcgtggtg catgctgaga
    33661 attgagtcgc tgtcgtaaat gcgtggatgg gtgaaatcaa catctgttgg atgatatgga
    33721 cgagagccgc aggcaataac aaatttttca gcggttagtg tttcaacgct gccgtccggg
    33781 caatccagcg ccaacgtatg ctcgtcaaca aagcgagcgt tcccctgcaa tatttcacag
    33841 tgattacgtt cgtaaaatcc ctgacgcatg cgcgtttgtt gattaatcac gttatcggca
    33901 tggttaagga tatcggcaaa agaagagcgg agcagtcggg aatggtcgct gtatagtggg
    33961 ttttgattga attctataat gcggctgacg gcgtgacgga gagctttcga cgggatggtg
    34021 ccccagtggg tgcaaccgcc gccaacattt tgataacgct cgataactgc gacgcgcgca
    34081 ccttgcttaa ccaggcccat tgcagcgcct tcgccgccgg ggccggaacc tattactatg
    34141 gcatcgtaat cgtaggaatg tggcatggta gggcttacct gttcttatac ataaaagcaa
    34201 cagaatggta acattttatc gcgggtaagc caattgatcc ccgtcattaa tctggctata
    34261 gcgtgagcgg cctttgcttt gtctgcctcc ttcttaaccc atttattcac tattaatcgc
    34321 tttttattat ggctgatagt tttatcttgc cgaagcgt
//
LOCUS       DAZKIF010000040        33944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZKIF010000040 DAZKIF010000000
VERSION     DAZKIF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.040"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..818
                     /locus_tag="ACHOTW_001911"
     CDS             <1..818
                     /locus_tag="ACHOTW_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9844025.1"
                     /translation="IILESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLL
                     KRIMSINDNVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIM
                     ANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHTIINSKKFKTSRA
                     ISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNA
                     RDSKHTGRMFSMHNCQINPSAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1008..1121
                     /gene="yhiY"
                     /locus_tag="ACHOTW_001912"
     CDS             1008..1121
                     /gene="yhiY"
                     /locus_tag="ACHOTW_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP9844026.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1184..2251
                     /gene="yhiI"
                     /locus_tag="ACHOTW_001913"
     CDS             1184..2251
                     /gene="yhiI"
                     /locus_tag="ACHOTW_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9844027.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2248..4983
                     /gene="rbbA"
                     /locus_tag="ACHOTW_001914"
     CDS             2248..4983
                     /gene="rbbA"
                     /locus_tag="ACHOTW_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP9844028.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4983..6107
                     /gene="yhhJ"
                     /locus_tag="ACHOTW_001915"
     CDS             4983..6107
                     /gene="yhhJ"
                     /locus_tag="ACHOTW_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9844029.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6180..6455
                     /locus_tag="ACHOTW_001916"
     CDS             6180..6455
                     /locus_tag="ACHOTW_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9844030.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6452..6811
                     /locus_tag="ACHOTW_001917"
     CDS             6452..6811
                     /locus_tag="ACHOTW_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9844031.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6861..7721)
                     /locus_tag="ACHOTW_001918"
     CDS             complement(6861..7721)
                     /locus_tag="ACHOTW_001918"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFP9844032.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(7753..8022)
                     /locus_tag="ACHOTW_001919"
     CDS             complement(7753..8022)
                     /locus_tag="ACHOTW_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP9844033.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(8012..9520)
                     /locus_tag="ACHOTW_001920"
     CDS             complement(8012..9520)
                     /locus_tag="ACHOTW_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP9844034.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(9513..10871)
                     /locus_tag="ACHOTW_001921"
     CDS             complement(9513..10871)
                     /locus_tag="ACHOTW_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9844035.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(10948..11229)
                     /locus_tag="ACHOTW_001922"
     CDS             complement(10948..11229)
                     /locus_tag="ACHOTW_001922"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9844036.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(11226..11699)
                     /locus_tag="ACHOTW_001923"
     CDS             complement(11226..11699)
                     /locus_tag="ACHOTW_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9844037.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(11724..12470)
                     /locus_tag="ACHOTW_001924"
     CDS             complement(11724..12470)
                     /locus_tag="ACHOTW_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9844038.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(12669..13070)
                     /gene="nikR"
                     /locus_tag="ACHOTW_001925"
     CDS             complement(12669..13070)
                     /gene="nikR"
                     /locus_tag="ACHOTW_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP9844039.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(13076..13882)
                     /gene="nikE"
                     /locus_tag="ACHOTW_001926"
     CDS             complement(13076..13882)
                     /gene="nikE"
                     /locus_tag="ACHOTW_001926"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP9844040.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(13879..14643)
                     /gene="nikD"
                     /locus_tag="ACHOTW_001927"
     CDS             complement(13879..14643)
                     /gene="nikD"
                     /locus_tag="ACHOTW_001927"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP9844041.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(14643..15476)
                     /gene="nikC"
                     /locus_tag="ACHOTW_001928"
     CDS             complement(14643..15476)
                     /gene="nikC"
                     /locus_tag="ACHOTW_001928"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP9844042.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(15473..16417)
                     /gene="nikB"
                     /locus_tag="ACHOTW_001929"
     CDS             complement(15473..16417)
                     /gene="nikB"
                     /locus_tag="ACHOTW_001929"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP9844043.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(16417..17991)
                     /gene="nikA"
                     /locus_tag="ACHOTW_001930"
     CDS             complement(16417..17991)
                     /gene="nikA"
                     /locus_tag="ACHOTW_001930"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9844044.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(18102..18689)
                     /gene="acpT"
                     /locus_tag="ACHOTW_001931"
     CDS             complement(18102..18689)
                     /gene="acpT"
                     /locus_tag="ACHOTW_001931"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP9844045.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(18691..19920)
                     /locus_tag="ACHOTW_001932"
     CDS             complement(18691..19920)
                     /locus_tag="ACHOTW_001932"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFP9844046.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(19917..20648)
                     /locus_tag="ACHOTW_001933"
     CDS             complement(19917..20648)
                     /locus_tag="ACHOTW_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFP9844047.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(20648..21112)
                     /locus_tag="ACHOTW_001934"
     CDS             complement(20648..21112)
                     /locus_tag="ACHOTW_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFP9844048.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(21109..22278)
                     /locus_tag="ACHOTW_001935"
     CDS             complement(21109..22278)
                     /locus_tag="ACHOTW_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFP9844049.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(22280..22864)
                     /locus_tag="ACHOTW_001936"
     CDS             complement(22280..22864)
                     /locus_tag="ACHOTW_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFP9844050.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(22861..25179)
                     /locus_tag="ACHOTW_001937"
     CDS             complement(22861..25179)
                     /locus_tag="ACHOTW_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFP9844051.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(25148..25753)
                     /locus_tag="ACHOTW_001938"
     CDS             complement(25148..25753)
                     /locus_tag="ACHOTW_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFP9844052.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(25750..26172)
                     /locus_tag="ACHOTW_001939"
     CDS             complement(25750..26172)
                     /locus_tag="ACHOTW_001939"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP9844053.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(26176..27852)
                     /locus_tag="ACHOTW_001940"
     CDS             complement(26176..27852)
                     /locus_tag="ACHOTW_001940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9844054.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(27843..28196)
                     /locus_tag="ACHOTW_001941"
     CDS             complement(27843..28196)
                     /locus_tag="ACHOTW_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFP9844055.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(28183..29544)
                     /locus_tag="ACHOTW_001942"
     CDS             complement(28183..29544)
                     /locus_tag="ACHOTW_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HFP9844056.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRAIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGMLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     AHLQWQNGGGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(29541..30122)
                     /locus_tag="ACHOTW_001943"
     CDS             complement(29541..30122)
                     /locus_tag="ACHOTW_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844057.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(30127..30378)
                     /locus_tag="ACHOTW_001944"
     CDS             complement(30127..30378)
                     /locus_tag="ACHOTW_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9844058.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(30390..30647)
                     /locus_tag="ACHOTW_001945"
     CDS             complement(30390..30647)
                     /locus_tag="ACHOTW_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFP9844059.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(30622..31443)
                     /locus_tag="ACHOTW_001946"
     CDS             complement(30622..31443)
                     /locus_tag="ACHOTW_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9844060.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILHQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRATDYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(31440..32162)
                     /locus_tag="ACHOTW_001947"
     CDS             complement(31440..32162)
                     /locus_tag="ACHOTW_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFP9844061.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(32203..33261)
                     /locus_tag="ACHOTW_001948"
     CDS             complement(32203..33261)
                     /locus_tag="ACHOTW_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP9844062.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(33330..33707)
                     /locus_tag="ACHOTW_001949"
     CDS             complement(33330..33707)
                     /locus_tag="ACHOTW_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9844063.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
BASE COUNT         7944 a         9011 c         9227 g         7762 t
ORIGIN      
        1 acattatttt agaaagtctt cagccttatt tagttattaa taaagacgca gtgacgccat
       61 gtacgttttt gggtgaccaa accggcgatc gatttagtaa tatctgtggc gatcaattta
      121 tcatcgattt gttaaaacgc ataatgagca tcaatgataa cgttcatgta ttagctggaa
      181 atcatgaaac gaattgcaat ggtaactata tgcagaactt cacgcgtatg aaaccacttg
      241 atgaggatac ctacgacggg ataaaagatt atcctgtatg tttctatgac tccaaataca
      301 aaataatggc aaatcatcat gggataactt ttgatgatca gcgaaaacgt tacattatag
      361 gaccgataac ggtctctatt gatgaaatga ccaatgctct cgatccggtc gaattagcgg
      421 caattattaa taaaaagcat catactataa ttaatagtaa aaagtttaaa actagccggg
      481 ctatctcttg tcgatccttt aatcgttatt tttctgtttc gacagattac agacctaaac
      541 ttgaagcact attagcatgc tcacaaatgt taggtataaa tcaagttgtt gcacataacg
      601 gtaatggtgg aagagaatgc ataggtgaaa cggggacagt tttaggtctt aacgcccgtg
      661 acagtaaaca tacgggaaga atgttcagca tgcataattg ccaaattaac cctagtgcgg
      721 ggcctgagat aaccactccc tggaagtctt accaacatga aaaaaacaga aatggactta
      781 tgcctttgat tagaaggcgc acaatgttac agctctgaac catagacaac atgaatatta
      841 atgttactat ctggttgaga ctgttgataa aacgtaataa ggaatgctct gttcctgcaa
      901 ctgaacaggc gtgaaacgct atccaacagg atggataccg ttttgtcagc cagttttata
      961 gatcacactt atgagtatgc gtgtttcatt tacggccagg aatgctgatg atgacaacat
     1021 tactacccgt tttcactaag ccttccccgt tggcgctcaa tgctctgcgc gctggtcgta
     1081 tttgtcgttt ccttcttatc ccggatggaa gaatccgctg aacgtcgttc ggcggcagtt
     1141 gtaacacgcg tcgtttgctt acagggatta tggcgggtcg attatggata agagtaagcg
     1201 ccatctggcg tggtggggtg tcgggttact ggcggtggct gctatcgtag cgtggtggct
     1261 gttgcgcccg gcaggtgtgc cggaaggctt tgccgtcagc aatgggcgca ttgaagcgac
     1321 ggaagtggat atcgccagca aaattgccgg gcgtatcgac accattctgg tgaaagaagg
     1381 ccagtttgtt cgcgaaggcg aagtgctggc gaagatggat actcgcgtgt tgcaggagca
     1441 gcgactggaa gccatcgcgc aaatcaaaga ggcacaaagc gccgttgccg ccgcgcaggc
     1501 tttgctggag caacgacaaa gcgaaactcg cgccgcacag tcgctggtta atcaacgcca
     1561 ggccgaactg gactccgttg ccaaacgtca tacgcgttcc cgctcgctcg cccaacgagg
     1621 ggctatttct gcgcaacagc tggatgacga tcgcgccgcc gctgagagcg cccgagctgc
     1681 gctggaatca gcaaaagccc aggtttcggc ttctaaagcc gctatagaag cggcacgcac
     1741 caatatcatt caggcgcaaa cccgcgtcga agccgcacaa gccactgaac ggcgcattgc
     1801 cgcagatatc gatgacagcg aactgaaagc cccgcgtgac ggacgcgtgc agtatcgggt
     1861 tgccgagcca ggggaagtgc tggcggcagg cggtcgggtg ctgaatatgg tcgatctcag
     1921 cgacgtctat atgaccttct tcctgccaac cgaacaggcg ggcacgctga aacttggcgg
     1981 tgaagcccgg ctgatcctcg atgccgcgcc agatctgcgt attcctgcaa ccatcagttt
     2041 tgtcgccagt gtcgcccagt tcacgccaaa aaccgtcgaa accagcgatg aacggctgaa
     2101 actgatgttc cgcgtcaaag cgcgtatccc accggaatta ctccagcagc atctggaata
     2161 tgtcaaaacg ggcttgccgg gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc
     2221 tgacgacctc gtggtgaggt tgccgcaatg acgcatctgg aactggttcc cgtcccgcct
     2281 gtcgcgcaac tggcgggcgt gagccagcat tatggaaaaa ccgttgcgct gaacaatatc
     2341 acactcgata ttccggcccg ctgtatggtc gggctgattg gtccggatgg cgtcgggaag
     2401 tcgagtttat tgtcattgat ttccggtgct cgtgtcattg agcagggcaa tgtgatcgtg
     2461 ctgggcggcg atatgcgcga cccgaagcat cgccgcgacg tctgcccgcg catcgcctgg
     2521 atgccgcagg ggctgggcaa aaacctctac cacaccttgt cggtgtatga aaacgtcgat
     2581 tttttcgctc gcctgttcgg tcacgacaaa gcggagcggg aagtgcgaat caatgagctg
     2641 ctgaccagca ccgggttagc accgtttcgc gatcgtccgg cagggaaact ctccggcggg
     2701 atgaagcaaa aactggggct gtgctgtgcg ttaatccacg acccggaact gttgattctt
     2761 gatgagccta cgacgggggt tgacccgctc tcccgcgccc agttctggga tctgatcgac
     2821 agtattcgcc agcggcagag caatatgagc gttctggtcg ccacggccta tatggaagag
     2881 gcggaacgct tcgactggtt ggtggcgatg aatgccggag aagtgttggc aactggcagc
     2941 gccgaagagc tgcggcagca aacgcaaagc gccacgctgg aagaagcatt tataaatctg
     3001 ttaccgcaag cgcaacgcca ggcgcatcag gcggtagtga tcccaccata tcaacctgaa
     3061 aacgcagaga ttgccatcga agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc
     3121 gttgatcacg tcaatttccg cattccacgc ggggagattt ttggttttct tggttcgaac
     3181 ggctgcggta aatccaccac catgaaaatg cttaccggat tgctgcccgc cagcgaaggt
     3241 gaggcgtggc tgttcgggca accggttgat ccaaaagata tcgatactcg ccgtcgggtg
     3301 ggctatatgt cgcaggcgtt ttcgctctat aacgagctca ccgtgcggca aaaccttgag
     3361 ttacatgccc gtttgtttca catcccggaa gcggaaattc ccgcaagagt ggctgaaatg
     3421 agcgagcgtt ttaagctcaa cgacgttgaa gatgttctgc cggagtcatt gccgctcggc
     3481 attcgccagc ggctttcgct ggcggtggcg gtgattcatc gcccggaaat gttaatcctc
     3541 gatgaaccta cttctggcgt cgatccggtg gcgagggata tgttctggca gttgatggtc
     3601 gatctctcgc gccaggacaa agtgactatc ttcatctcca cccactttat gaacgaagcg
     3661 gaacgctgcg accgtatctc tctgatgcac gccggaaaag tgctcgccag cggtacaccg
     3721 caggaactgg ttgagaaacg cggagccgcc agtctggaag aggcatttat cgcctatttg
     3781 caggaagcgg caggacagag caacgaagcc gaagcgcctc ccgtgataca cgacaccaca
     3841 cacgcgccgc gccagggatt tagcctgcgc cgtctgttta gctacagccg ccgcgaagcg
     3901 ctggaactgc gacgcgatcc ggtacgttcg acgctggcgc tgatgggaac agtgatcctg
     3961 atgctgataa tgggttacgg catcagtatg gatgtggaaa acctgcgctt tgcggtgctt
     4021 gaccgcgacc agaccgtcag tagccaggcg tggacgctca atctctccgg ttcccgttac
     4081 tttatcgaac agccgctgct taccagttat gacgagcttg atcgtcggat gcgtgcgggc
     4141 gatatcacgg tggcaattga gatcccgccc aatttcgggc gcgatatcgc gcgtggtacg
     4201 cctgtggaac tcggtgtctg gatcgacggt gcgatgccga gccgcgccga aactgtaaaa
     4261 ggttacgtgc aggccatgca ccagagctgg ctacaggatg tggcgagccg acaatccaca
     4321 ccctccagcc aaagcgggct gatgaatatt gagacgcgtt atcgctataa cccggacgta
     4381 aaaagcctgc cagcgattgt tccggcggtg atcccgcttc tgctgatgat gatcccgtca
     4441 atgctaagcg cccttagcgt ggtgcgggaa aaagagctag ggtcgattat caacctttac
     4501 gtgaccccca ccacccgtag cgaatttttg cttggcaaac agctgccgta catcgcgctg
     4561 gggatgctga actttttcct gctctgcggc ctgtcggtgt ttgtgtttgg cgtaccgcat
     4621 aaaggcagtt tcctgacgct caccctggcg gcgctgctgt atatcatcat cgccaccgga
     4681 atggggctgc tgatctccac ctttatgaaa agccagattg ccgccatttt cggtacggcg
     4741 attatcacgt tgatcccggc gacacagttt tccgggatga tcgatccggt agcttcgctg
     4801 gaagggccgg gacgttggat cggcgaggtt tacccgacca gtcattttct gactatcgcc
     4861 cgcgggacgt tctcgaaagc gctggatctg actgatttgt ggcaactttt tatcccgttg
     4921 ctgatagcca taccgctggt gatgggctta agcatcctgc tgctgaaaaa acaggaggga
     4981 tgatgcgcca tttacgcaat atttttaatc tgggtatcaa agagttgcgc agtctactcg
     5041 gtgataaagc gatgctgaca ttgattgtct tctcgtttac ggtgtcggtg tattcgtcag
     5101 cgaccgtcac gccaggatcg ttgaacctgg caccgatcgc cattgccgat atggatcaat
     5161 cgcagttatc gaaccggatc gttaacagct tctatcgccc gtggtttttg ccaccggaga
     5221 tgatcaccgc cgatgagatg gatgccggac tggacgccgg gcgttatacc tttgcgataa
     5281 atattccgcc taattttcag cgtgatgtcc tcgccggacg ccagccagat attcaggtga
     5341 acgtcgatgc cacgcgcatg agccaggcat ttaccggcaa tgggtatatc cagaatatta
     5401 tcaacggtga agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat
     5461 cgctggaaac ccggatgcgc tttaacccga accttgatcc cgcgtggttt ggcggggtga
     5521 tggcgatcat caacaacatt accatgctgg cgattgtatt gaccggatcg gcgctgatcc
     5581 gcgagcgtga acacggcacg gtggaacact tactggtgat gccgataacg ccgtttgaga
     5641 tcatgatggc gaagatctgg tcgatggggt tggtggtgct ggtggtatcg ggattatcac
     5701 tggtgctgat ggtaaaaggc gtgttgggcg taccgattga aggctcgatc ccgctgttta
     5761 tgctgggcgt ggcgcttagt ctgtttgcca ccacgtcaat cggcattttt atggggacaa
     5821 tagcgcgttc aatgccgcaa ctggggctgc tggtgattct ggtgctgctg ccgctgcaaa
     5881 tgctttccgg tggttccacg ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc
     5941 tgatcatgcc gacgacacac tttgttagcc tcgcgcaggc cattctctac cgtggtgccg
     6001 gattcgaaat cgtctggccg cagttcctga cgctgatggc aattggcggc gcgtttttca
     6061 ccattgcgct gctgcgattc aggaagacga ttgggacaat ggcgtaagga agggaaactt
     6121 gacttcatac tgtgatgtca tactattgat gtcataaagt gaagtcataa ggacatgcca
     6181 tgagaaccaa ggtacaggct ttacggaaga aacaaaaaaa tacattggat caaatattta
     6241 aaactcctgt tcctcaaggg atcaagtggt ctgatataga gtcactggtt aaagcattag
     6301 gcggagaaat taaggaagga agaggttcgc gttgtaagct catactaaat atgagcgttg
     6361 cgtgtttcca tcggcctcat ccgtcgccag ataccgataa aggcgctgta gaaagcgtgc
     6421 gtgactggtt actaagtata ggggtaaaac catgatcaaa ctgaagacgc caaactctat
     6481 ggagattgcc gggcaacctg ctgttatcac ttacgtgcca gaactgaatg catttcgtgg
     6541 caaattcctg ggcttgtcag gttattgtga ttttgtctct gacagcattc aggggctgca
     6601 aaaagaaggg gaactttccc tgcgagagta tcttgaggat tgtaaagctg cgggtattga
     6661 gccgtatgcg cgtacagaaa agattaagac ctttacgtta cgctatccag aatcgctgag
     6721 tgaacgcctt aataatgcgg ctgcgcagca acaagtctct gttaatacct atattattga
     6781 aacactgaat gagcgtttga atcatttata acttatagcg gcccaacaat ttctgttgcg
     6841 ccgctatatt tttgtggctc ttaagatgcc tttctaacca accgatgccc ctcattcatc
     6901 atcaggattt tgctatacac atcttcctcg acggcgtttt ttgctgatgc catgactcgt
     6961 gccagattcg cctcgttgtg attattcaca tacgctttgc cgacggcggt gataaacgct
     7021 ttgcgcagat cgctggcgat atttacttta gcgacgcgat aattaacaaa gctgcgtaat
     7081 atctccgggg cgataccgga gccgccgtga ataaccaacg gcaccggaca gacttcggca
     7141 atgcgcttca gcagggggat atcgatacgc ggtatatcgt ccaggccatg aacgttgcca
     7201 atagaaactg ccagcatgtc gcaaccggta cgctcgacaa aggtcttcac cttttccggt
     7261 tccgttttgc aatcggcttc gctgacgtga tcatcttctt tgccgagaat cgcccccagc
     7321 tccgcttcaa ccggtacgcc ataggattta cagaaatcga cagcttcctg ggtgaaagcg
     7381 atattctctt caaaagagaa tgcggcaccg tcaatcatga ccgaggtaaa gcccgcgcgc
     7441 accgcctgtt taacatcctc ctgggttttc ccgtgatcca gatgaagact gaccggaaca
     7501 tccattttat cggcgtgacg tttcacgatt tcataaatgt aatcgtatcc cgagagttgt
     7561 gcattggttg gcgcaatctg aatgaagttg ggtaatcccg aacgctcaat agcgtttaaa
     7621 attgaaattg tcgtctccag attggtggta ttaaatgcgc ccgctaatac ccgtttttcc
     7681 tgaatgcggt cgagcagtat ccgtccatta actaatggca tattgaaaac ctcatctttt
     7741 aatatcagtt aattattcag cttcaccgaa gcggtcgcga aataagtcgg tgagagtctg
     7801 tatggcctcg ttttcatcat caccgtcgca gcgtatttcc accgtttttc cgccttttat
     7861 tccggcgcgc ataatattca tcatgctggt ggcattaata tcattaccct catagtgcag
     7921 cgtcacttta cagctatatt ttttggcggc tttcgccaga caggccgccg gacgtgcatg
     7981 caggccagta ctgtttctga cctccacggt tttattcaac atagtgttcc tctcgatgat
     8041 gtaatggcat cacgccttct tgttgtaatt tcaactgata ccagcttttc catttgtgct
     8101 gataacgctg taattgatgg tatctcgccc gattcggtaa caaacgttgg gctatagcga
     8161 ctggcggcgg tggaatatcc ggtgcggcgg ttaataatgc tccgacagaa accgcctcgt
     8221 taaatgcaat tgcttcaacc ggacgctgta aaatatccgc ctttaattgc agccaaagag
     8281 gattatgggt tgccggaccg atcactttta acgcgctggt gccaggcacg ttaaaacagg
     8341 cgaagcagtt ggcaaattcc atggttaatc ccagtgatac gctggcaatt aacatgtcga
     8401 tacttaacgt atcgccaagc ccataaaata atccacgggt atggcgattt ttatagggcg
     8461 aacccgaacc gcgaagatgt ggaataaaga cgggaatgtc atcgagcaac caattccccg
     8521 ccagataatc agcatgtccg cgagtaagtg atgcggcgat ctcctcatcg gttagccgga
     8581 acgtgttgcg cagccactcc agcgcgaaac cgcccacggg cagcgacgca aacagggtga
     8641 actggttggc aagtgagtaa cagccgtttg ccagcttgtc acgtttggcc tgttcatcca
     8701 gcgtcggttg tgtatccagt tgcagaatgc cttccgtggt ccccgtcgag ttaaggatat
     8761 cgcctggcat catctgcaac gctcgcgcac ccaccatatg gtcatgtccg gccagcgtca
     8821 cgctgaccgg ttgcgagaac ccaagcgtct tgcaaagtga ctcgctgacc catccggcgt
     8881 gctcgcctgg ctgcaccaat ggcgcaaatg ccgtacaggg aacgtgtaac aacgccgccg
     8941 ctttcgctga ccattcgcct ttgcgcacgt ccagacacag cgtgcggctg gctaaggtga
     9001 tatccgtgtg ctgttcgcca gtcagccgcc agagcagcac ttccggcgca tgtagccagc
     9061 gcaggccgcg gcgattacgc agcgggtaat gttccagcaa ccagcgcatt ttgaaggcag
     9121 agtaattgct gtgtagtggt agaccgcaga tgtcataaag tgccgcactg tctgcctcgc
     9181 taagcgttgc cagatactct tcaccgcgac ggtcatacca tgccagcatt ggcgtcagga
     9241 tctcgccatg ctcgtcaagg aacacgcctg attcgccaaa actggcaatg ctgatccgtc
     9301 tgactggcag cggcgaagcg gcgttcagtt gcgctatcgc ctggcggacc tcctgccaga
     9361 gggcgtcgat atcgaaatcg acatcgccct gtggggagat ctgctttgcc gtcacgaatt
     9421 tatgcgcgcc caacgtcgtt gcgtccaggc aggaaaagca ggtgactttg caattggtgg
     9481 tgccaatatc gataacgata gctgcgctgt tatcaggcat aaaccgtgct ccgcgtcatt
     9541 cggaaaatgc catacagtac cagggcgata accggcacca gaccaatggc gatgagattg
     9601 ccgttgaaga tttccagcag ccagaagcgg aacgggttac cgccatcaag aaaactggaa
     9661 acctggccgc tggagccttc ggcaaatttg aagttaacgt ctttcgccat tccggtaata
     9721 aacggcgcga ttttggtagc aatccatagg tcggcaataa tcaccgggat cgccgccaga
     9781 acggtgcgga aaatattgcc acggctggct aaagcaatca tcgacgccat taccgccagg
     9841 ttggcaaggt cgccaagcgg caggacttca ttacccggaa ggacaaacgc gataaagacc
     9901 gagattggcg ttagcagcag ggcggtggag ataatcgccg gattacctac ggcaacggcg
     9961 atatccaggc cgatatagag atcgtcacgg tcagggtatt tggcattgag atacttttta
    10021 atagcttcag acagcggtaa taaaccttcc atcaggatgc gtaccatgcg tggcaggatg
    10081 aacatcacac cgccaatgct gatgccaagc agcaatactt ttttgaaatc gtatccggca
    10141 attacgccga gcagaatgcc cagaatagtg ccaaccatca tcggttcgcc gaagatgcca
    10201 aactttttct gtacggtttc cgggtcgata tgaatacgat tgaggccagg gatatggtcg
    10261 atgattttgt cgaccagcag accgaccggg aagaacacca ccgaagagag cgtcggcaat
    10321 gagatccctt ccagtccaaa gtatttttgt accagtggcg cagaccagtc ggcgagttta
    10381 agtactacca ccgcagcaat cgccgccgcc agcaaaccaa agaacaggct gccggtgctg
    10441 taataaacca acgtaccagc aagggcaaaa tgccagtagt tccacatatc tacgtcaacg
    10501 gtacgggttt tatttaacgc cagcatcgcc acgttaatca gaatggtcag gggaataaca
    10561 aacggggcaa ttggcgaacc ccatgtaata gcggaaagcg gcggccagcc taaatctacc
    10621 accggtaaat taattccggt gcgttcaacc atcgcctggg ccgctggccc gacattgctg
    10681 gtcagaatat cgaaaatggc ataaataccg acaaaaccaa tgccgactgt taatgacgaa
    10741 cgtaaggctt tagcgggttt tactttaaaa cagagagcaa gaataaataa caccaacggc
    10801 aacattaccg tcgggccaaa tcccaatata tattgcacaa tattatagag ggtatgcgcg
    10861 atatcgttca taataaacct ctttttcagg cgagagcgca cctcgcgtta gcgcctgatg
    10921 gagtgggttg aaattaatta ttattaatta ctttttaagg atagaaacaa tttgcgccag
    10981 tactttttct tcgccaatac cggtaataat cggcaagccg ttcaccaccg ggacgtcgag
    11041 ttcataaggc actttagttg ttgaaactac cagtgctacg ccgtcagtat ttccaggtaa
    11101 agaggcaacg ttggtttggg aataattgac gttaagacct tgctctttgc aatattccat
    11161 gactttttct gttaccgccg tggatgtggc aatacccgtg gcgcagacaa ataaaatggt
    11221 ttgactcata tttcacctcg ttctttcatt ttgtttaaga ttactgtgtg tatttctgtt
    11281 gtttccatcg caagcagggc ttgcataaag ggcgtatcct gaatcatttc cataatccag
    11341 cccagggcat ttaattgttt attgctttcg cttaaggcga gcaaaaaaat caccctgaca
    11401 ggtacggggt caggatcgcc gcccatatcg gcaaactcta ccggttccgg caggatgccg
    11461 acggcaattg cattgtgcct gacatgttta tggtccgtgt gcggaatagc gacgcacggt
    11521 ggttcggtag gcagccccgt tgggtacgtc gcctcacgtt tcagtacgtg atcggcaaaa
    11581 tcagggacga ccagctcggc ggcaaccaac ggttgcgcta actggcggat gatctgttca
    11641 acgcttaacc ctgccggaag tcgttgaata taattttcct gaaaatgtat gccttgcatt
    11701 ttattcctct taaaaaagtg tacttaactc acttcccgcc gttgcagtac ggtgccaaga
    11761 taatatttat ttccttttaa ccagacgttg ccaaactcaa tgggtaatcc tcgggagaaa
    11821 aagactaact gctccagatg caaaacgggt gcgtcttcac tgatatctaa aaaatgacca
    11881 cgttcattac caattaatcg cgcggcatag cgactttccg agtaacgaat ttttctttgc
    11941 gacaaacttt ctattgttgg aaataaattg tgttgattaa aatcgatttc agcaataccg
    12001 gggcatagct caatattgat acggttctct atcagcatcg ctttttcatc accaattgag
    12061 cgtaagcgtt caaggtaaag aatatcctga ccgggcgtta ttcttaattt ctctgccaca
    12121 taacgattag ccgattcaat ccgtgacgta ataacttcag tggtaaaatg tattttctgg
    12181 ctttccagcg attccgcgaa tgacaataat ccttcaccta acggatatgc cacgttctct
    12241 tttttgacaa atgttccctt accctggacc tgtattaaca cgccttcttt taccagttgg
    12301 gcgaccgctt ttttaacggt tccccgactc acgtccagca tatccataat ctggtattcc
    12361 gaaggaatgc gatcgtcttc caccagatct ccggtataaa tactttctct tatccactcc
    12421 accacttgtt gatagagcgg tatttgtgac gacttactta atgatttcat ggcgcgcacc
    12481 tgaacattta cagttgtcta tggacaatca tggacaactt caagcgtata gtaggtgaac
    12541 gggagaaaaa gaggatcgaa gcgtctcaca ttttgaggga ccaaatcgtg atcaaaaaaa
    12601 tgtgacgtga gtcgatattt gtcagaagtt tactgcgccg taaggaaata acggcgcgtt
    12661 ggtaggtgtt aatcttcctt cggcaggcac tgcaaatgcc cgtgccgcac gccgcgctgg
    12721 gcgataacgt catcggcaaa atgctgcacg tcacccatgt cacctttcaa cacggcgatt
    12781 tccagacagt cgtcgtggtt gatgtgcaca tgtagcgtgg cgacggagag gtcgtggtga
    12841 tgatgctggg tggaaacaat acggctggct aagtcgcgtt tttcgtgttc atacacatac
    12901 gacagcaccg cgaaaccttg tgtgccgtgc tgctgggtag cttcctgcgc cagggcgcta
    12961 cgcagaatat cgcggatagc ttcggaacgg ttgttataac cacgacgctg gctcaggctg
    13021 tccagcgtct ccagtaaatc gtcatcaagc gtgatggtga ctcgttgcat ttgcgttaaa
    13081 ccttttctgt ggcgcgacgg cgcacgggga atgcgggtaa tatcgcgttt tgtagcacac
    13141 gtccggcgtc agaggaaaag gttaatttct ctcccaccgc ctgggtttcg acgatttgtc
    13201 cgttgtccat caccatcact cgctggcaaa agcgttccac caggcgtaag tcatgagtga
    13261 tgaacaggca ggcagtgcca aactgttgtt gtagcttttt cagcaggcga atgacgcccg
    13321 cctgtaacac gagatcaagg ttagaaacgg cttcatccag aatcagcaat ttcggttcga
    13381 ccgccagcgc gcgtgccagg cagacgcgct ggagctggcc gccgcttaac tgcggtgggc
    13441 gcttgtcgag cacgctgtcg tcgagatcga ccgcgtgcag catttcgctg gcacgcgcca
    13501 gttgttcgga ttttttcagc gatagcaggt ggcgcatcgg ttcccgcagg atctcgcgca
    13561 cggttttgcg cggattcacg gcgctgatgg agtcctgaaa taccatctga atatcgcggc
    13621 ggaacgcttt acgctgggcg cgattgagct ttgccaacga ttcgccacgc cagctaatat
    13681 ttccctgtga gggcgattct aagcctacca gtagccgcga gagagtgctt tttccgcagc
    13741 cgctgcgtcc cagcagggcg acggtttcac cgcttttcag ggtcagggaa acgttattca
    13801 gcaccgcctg gtgctgatgt tttccgctaa acccaccgtg ggcatagtga tgggaaaggt
    13861 cggagacgtt aagtaaagtc atgatgccag ctccataccg tagagggcga gatgagcgga
    13921 aaccaggctg cgcgttaccg catgtttggg ggcgttaaac agcgtttcga catcgccctg
    13981 ttcgacaatc ttaccgtcag acattaccgc tacgtcatcc gccagacgcg ccaccacgcc
    14041 catatcatgg gtgaccagca gcatccccgg cgcctgtttt tgcataatgc tttccagcag
    14101 atcgagaatg cgtgcctgtg ctaccacgtc gaggtcggtg gttggttcat cggcgatgat
    14161 aaacggtgat tcgcaaagca ccgccatcgc aatcatcatg cgctgcaaca tgccgccgct
    14221 catctcgaac gggtacagct tcagcacgcg tgcggcgttt tccagcccca ccgcttctat
    14281 ggcagcggta agcgtagcgt catcggcggg tttccccagc gccaggcacg tctcccgcgc
    14341 gtgggtgtgc atggtgtgca gtggattaaa ggcgctgcgc gggttctgca tgatggtggc
    14401 aattttgatg ccgcgcaggg cgcagggaga aaccggtttg ccatcggcta aaatttcccc
    14461 cgccgtctgg cgaacgcctg cgggcagaat tcccagcgtc gcggcgcagg ttaatgattt
    14521 cccgctgccg ctaccgccga ctaacgccag cacgcgcccg cgtttcaggg ttaacgacac
    14581 gccgtgcacc agcggctgcg cggcctgtag cgcgatatta cgtagttcaa tctgttgcgg
    14641 cattagtgtg cgtgctccgt caccagatga ggatccagat gatcgcgcag tgcgtcaccc
    14701 accaggttaa aggccatcac gctgataaac agcgccagcc ccggccagaa catttgcagc
    14761 ggctgggtcc agatatactg gcgcgcgtcg ttaatcatca cgccccattc ggcggtcggc
    14821 gcggtcacgc cgaggccgag gaaggacatc ccggcaacgt gcagcatcat atggccgata
    14881 tccagcgttg ccagcaccag cagcgaaggg atcaccgcac ctgccagatg atcaacaaac
    14941 actcgcacat ggcccgcgcc agaaagccgt gatgccagca caaactcgcg ctggcgcagt
    15001 gaaatcacca ggctgcgcac catgcgtgca taccacgccc agtgcgacag ggcgatggcg
    15061 ataattacgt tggtcagccc agtgccgagc acgccaacca taaagaatga cagaatcgag
    15121 gtcgggaagg tcataaacat atcggcgacg cgcatggtag cctgatcaac gcgcccgcca
    15181 atcagcccgg cgctgccgcc aataaccagc cctaatgtca gcaccagcag caggcaggcc
    15241 attaccgaac cgagcgacac acgggtcgct gccatcagcc gcgagaaaat atcgcgccct
    15301 aagtgatcgg tgcccaacca gtgctgcgca tccggcgaaa gcaggcgcga cggcaaatca
    15361 atcgcctggg ggtcatacgg cagccaccac tggctggtga gcgcaatcag cgccagcagg
    15421 gcgataatga tcagcgccag gcgtaccgac cagcgggaag agaggaaaaa gttcacgcgt
    15481 gcgctccttc atgacgacga atgcgtgggt ccagtgcggc gttgagcaaa tcgacaatca
    15541 aattacagac cacaaaaacc accaccatca tcagcgtaaa gcactggatc accggatagt
    15601 cacggttaaa aatcgccgac accgcatagc gcccgacgcc gggccaggta aagatgtttt
    15661 cgataatcat cgtcccgcca atcagttcgc caatgtgcat ccccacggcg gtgatcatcg
    15721 gcagcgaggc attgcgcaga atatgacgac gctcggtctg tttgtcattc aggccgcgta
    15781 gccgcgccca ggtgacgtga cgctgaccgg cgacgtccag catactggcg cgcagcagcc
    15841 gtgcgttaat cgccagcgac ataaaggcaa tggaaaccgc aggcaaaatg atgtgctgcc
    15901 agccgccgta gcccatcgcg ggtagccatt gcagatacac cgaaaacgcc atcaccagta
    15961 aaaatgccag ccagaagttg ggcatcgaca cgccgagaaa cgcgatgaaa cgtacggcga
    16021 aatccggcag acggtcacga tggcgcgccg cccagatacc gagcggtacg gaagtgagca
    16081 gaattaatac cagcgccgca cccgccagtt ccagcgtggc gggcaggaag ttcagcatat
    16141 cgtccagcac cgggcgctgc gtggcaaatg agatgccgaa atcaagatgc agcgccttcc
    16201 acaaccaggt gccgtactgc acatacagcg gctgatccag tcccagcatg gtgcgggtag
    16261 aggccagcat ctccggcgtc ggcggcaggt tagacagacg caaataatcg agcgccgggt
    16321 cgccggtacc gaggcgcagc atcagaaaaa tgatcaccga ggcggcgagc accatcggga
    16381 tcagcagcag aaagcggcgt aatacgtaac gcaacattaa ggtttcaccg gtttaatctg
    16441 ttcgaacgga atttcggtgg cgatcggcgc gtaggggatg ttacccagct ccggttttga
    16501 taccaccatc attgagatgt aactgatagg cagataaacc gcctcgtcat gcagacgggt
    16561 cagaatgtcg cgatacagtg cctgacgttg tgcttcgtca tgggtcgcca gcacttcgcc
    16621 gatctcttta tcaatcagcg gcttgtcggc taatccttgc tgtgcctgga agtcagcgtg
    16681 cgacggtacg cgcattgaac tcaggaaggc gtgtggatcg tatggcgcgc cccaggtgcg
    16741 gtggaaaatc atgccaaaac gaccgtcgcg ctgacgcgca tagatactgc tctcttcttc
    16801 gccaatcagc gagacatctg cgccaatctg gcgcatatca gcctgaatga tttccgccat
    16861 cgatttgctt aacgcatcgg tgccgatgaa cgaaagttca atgtgcagcg gctgaccatt
    16921 tttctcgcgg atgtctttgc ccgcaggcag cgtccaaccg gctttttcca gcaacgcttt
    16981 cgctttctgc ggatcgtact ggcgcggttt caggccgagg ttggcgtagg gcacagaagg
    17041 ggcaaacagg gtgtcggcga cctgctgggt gccatacaac gcgttatcaa tcagcgattt
    17101 tttgtttacc gcgtaattaa gagcttcacg tactgccagc tcattggtgg gggctttggc
    17161 ggtattaagc gccagcatca cggtttcgat cggctgtgac agttgggtat ggtaagctgg
    17221 gttctggcta aagcgggcga aggtatcgag cggtaataac ccttcgtttc cgtacagcag
    17281 gtcgatatcg ccagtttcaa acgccaccgc gcgggtagtc gggtctggga tgacgttaaa
    17341 agtgatcttc ttaattgccg gcttttcgcc ccagtagttt tcgttacgga cgaagacatc
    17401 gtactgattc agtttcgatt cctgcaaaac ccacggtccg gtgccaatcg gcgctttaat
    17461 cccgttcatg gtttcatggt ttttaaactg cgagggggcg ataaagcgga aagggcgcgg
    17521 cagggccagt tcttgcagga aaggatagta ggcgcttttc agggtgattt gcagctctgt
    17581 tttactgagt gctttaacat caacaatctg gtttgccagc tccagccagg cgtgacgttg
    17641 acggttatcg agcactgcgc ggaagttttc tgccgccgcc tctgcatcaa acggttcgcc
    17701 gttagagaat ttcacgtcat cacgcagggt gaaggtccag gttttaccat cttctgaatg
    17761 agtccagctt tttgccagcc acgggatcac cgaaccgtct gcctgatatt tcaccaatgg
    17821 ttcataaacc atgctctggg cgaacatctg gttaggcgtg taaaggtgcg ggtttagtgg
    17881 cccgacattc accggccagg cggtggtgat ttcatctggt gcagcggcat ggacgataaa
    17941 agacgcacaa gccagcagcg caaatagagt gcggcggagt gtggagagca tggtaacccc
    18001 aatggattaa aatagatggc ggaaataagt atgacgattt taagtattcg tcatactgat
    18061 tacctgttct ggatcaatag atgggcagtc gggtgggcgg atcagttaac tgaatcgatc
    18121 cattgcacac tgtcggcggt gagcgtaaag ggagtagggg tgcagatcgc caggcttaaa
    18181 ttttctagct gacaatggct gactgacagc gaggagtgat aggtgctgtc tacgctgacg
    18241 atttgccagg cgctgccacc acgctgttta acgatggctt ctttgcgcgt ccagatgcgc
    18301 cagaacattt ccagttgctg atcaggatgc acggcgtcca tctcagcatg ttccccgagg
    18361 ctgaacacgg cgttcgccag ccagcgccag ttggcgcgcg ggcgaatcac ttcgatatcg
    18421 cagccgactt cgccttcatc actcaacagc agggcgatat cgtcaccgct atggcttaag
    18481 ttgaaccata gcggcgtttc cggcgcaaat gcaggtttgc cttgttcgcc atagatgatc
    18541 tccggtagcg gggaaagcgt gtgcgaaagc aatgcacgcc ccgccagcca gcgttcgcgt
    18601 cgtggacctt gcggtgcttg ctcgcgtaaa cccggtggca gtggagctgc gcttaaggtc
    18661 gaaactttcc ccagaactat ccgatacata tcagggccaa cgtttaatga tgatggatgt
    18721 attaatgccg ccaaaggcaa agttgttact ctgcaaaaac tcacaatcaa ccttgcgggc
    18781 ttcatgcatg atgtaatcca gcgcgccaca attcgggtct ggtttattca ggtttagcgt
    18841 aggcgcaaac caaccttcgc gcatcatttg cagactcatc caggcttcga gcgcgccaca
    18901 ggcaccaagc gtatgaccaa aataactttt cagcgaggag agcggcacat tatcaccgta
    18961 aatggttgct gtcgccagac tttctgccat atcaccgcga tcggtcgccg taccatgcgc
    19021 tgaaatatag ccgatatctt gtgcgcttaa cccggcgatt ttcagcgatt gctccataca
    19081 atactgcatg gtttcgcgct gcggctgggt aatatgtgcc gcgtcgcagt tagtcgcaaa
    19141 gccgacgatt tcaccgtaaa tggtcgcgcc gcgcgctttg gcgtgctcca gttcttcgag
    19201 aatgagcgta cctgcgcctt caccaatcac cagcccgtcg cggttttcat cgaacggtga
    19261 aggtgtggtt ttcggcgcat cattgtgctg gctggtggcg aaaagcgtat caaataccgc
    19321 tgcttctgaa ggacataact cttcggcacc accagcaacc atcaccgttt gataaccgtg
    19381 gcgaatggct tcccacgcat aaccgattgc ctggctgccg gaagtacagg cgctggaggt
    19441 tgggatcacc cgcccgcgca ggccgaaaaa gaggccagta ttaacggcgg tggtgtgcgg
    19501 catcatctgc acataggtgg tgccagtgat gttattggtg tgcttttccg tcagcatggt
    19561 ggcgaactcg cttaccgggc cggtactgcc ggtcgatgaa ccgtacgcaa tgcccgtttc
    19621 gccgctggtt aatatcggat cgccaatcaa ccctgcctgc tctaacgcca gctcgctggc
    19681 acgggtcgac atttgcgaca cgcggcccat cgcacggata cgcttgcggg tgtagtgctc
    19741 cggtagcgtg aaatcatcaa caggcgcgcc aagcagggtg tgcagcccat cgtagacctg
    19801 ccactccggc attttgcgca ccgcattttc atatgccagt aagcgtgcag aaacgtcctg
    19861 ccagttttca ccaaaggcag tcacgccgcc cattcctgta atcactacgc gacgtgtcat
    19921 aacatccctc cgttgatgga aataacctgg cgggtgacgt aacccgcaat atcagacatt
    19981 aaatagctgg caagcccggc aacttcttcc gcctggccca tgcgtttcat ggggatcatc
    20041 gacattgcct ctttcagggc gctctcttcc atttcgatca tcccggtatc aataagcccc
    20101 ggcgcgatgc agttgacggt gattttgcgc ttcgcgagtt cgatagccag cgcttttgtt
    20161 gcgccgataa ttccggcttt ggcagcgctg taattcacct ggccgcgatt gcccattacg
    20221 ccggaaaccg aggatagcgt gatgatgcgc ccaccctggc gtgcgccgat catcggcata
    20281 atgcacggct gaatgacgtt gtagaaactg tcgaggttgg tgtggatcac cgcatcccag
    20341 tcgtcatcgc ttaaagccgg aaaagcggca tcgcgggcga tcccggcgtt actgaccacg
    20401 ccgtaccagg caccgtgttg cgcgatttcg tgctccagca cttcccgaca ctgttcgcgg
    20461 tttgcgacgt cgaaactgag caaacgcccg ttacccccat tggcgacaat ggcattcagc
    20521 gtttcctgag cgcctgtggc gtcacggtga tagtgaacgc caatgttaaa gccgtctgcc
    20581 gccagttggc aggcgatggc gcgaccaatg cctttgctgg caccagtaac cagaactgaa
    20641 cgactcatca ggacgctcct tgttgaaaca gggtggtaag ttcttccgcg gtcggctgga
    20701 aggtgttaat gcggcccgtc gcctcgccgt cgttgatgct gcactcaaaa ctgccgaagc
    20761 gatcgtcttg catcagcagt ttgacggtaa tggtcagtgt ctggcctgcg ggcagaatgc
    20821 cagtggtgca aagcaattca cgcgcgccca gtatcatccc taactcaata ctgtttttgc
    20881 cctgctggtg gcgatgccag ccagaccaga cgccgacggt ttgcgccatc agttcaaggg
    20941 taaaccagcc ggggagattg ccgtccgggt cgagaaacgg tgccagtacg ccactgggcg
    21001 aaaccgtcac gcggcacacc gcgctgtcat cgctgacgct caccacgtct tccagcagta
    21061 acatgggcgc gtcgtggggc agataagcgc cgggggataa atagtggctc acgaaaccct
    21121 cccgagcaga atgctggcgt tattgccgcc aaaggcgaag gaattggaca ggatcaccgg
    21181 acgtgccagc ggctgcggtt tttcgataat gccacacggt ggcagcgtgg gatcgcgcgg
    21241 cgacaggctg aagtcctgag cgggcagcgg taaatcacgc tgtaaaatca acatactaat
    21301 tgccgcctcg gtaatgcccg ctgtgcccag cgtgtgtccg gtcaaatgtt tggtggagct
    21361 gcacggtacg cgttcgccaa acagggcgtt aaccaccatt gattcaatct gatcgttgag
    21421 ttgtgtggcg gtgccgtgca ggttgatata gccgacgtca tctggcgtaa gctgcgcatc
    21481 ggtcagcgcc tgattaatag cgcggatcgc tccttcgccc tgcggatgcg gggctgaaat
    21541 atggtaagcg tcgctcgatt caccaacgcc cagcaacgcg atgggctgcg gttcccgggt
    21601 cagcagcatt agccccgccc cttcaccaat ggtgatacct gcgcggtcac gaccaaacgg
    21661 ctgacataag gttggcgaca gcgattcgag gctgtgaaaa ccgttaatcg gcatccggct
    21721 cagggtgtct gcgccaccca caatggcggc atcgaccagc cccgcttcga taagcctgcg
    21781 cccgctgatt atcgctctgg cgctggaaga gcaggcggtt gaaagggtat aggccgggcc
    21841 gtcgagcgcc agccagtggc tgagaaaacg cgacggatcg ccgagttcct gttgcgcgta
    21901 gtgccatgtg gtgctcggct gcccgtgggt gcgcaggtta acgtgcgtgt cgccttcatg
    21961 caggccggag gtgctggtac caagcacaat cgcaatgcgc tcgcggccat atttcgcaat
    22021 ggcgtcatca acctgcggct ggatctgcgc cagcgccgcc agcaggatct gattgttgcg
    22081 cgagcggtgc gcagcgaatt tttccgggat aagcggaagc tcgccatcca cgccagccag
    22141 gaccgcctgc ggatgcccct gcaaccagcc tgcgcgcggg cgcatgccgg gagcaacacc
    22201 gcgagtcagg ttggttgcga tttcgtcaag gttatttccc agggcgttga tcatgccgac
    22261 ggcggaaata taaatcatat cagtcaccta agtattgaat ggtgatgtgg tatttaaaga
    22321 catgctgctc aatgctgatt ggcacgcgtt tgcccttgcg attcagatag gtgatttccg
    22381 tgaccagttt gccgctggcg ttacgcagct cgcgtttgtc accgttgtcg cgaagtgtcc
    22441 atcctgcggg aagttgcggt tgccaggcgc taatcggcca gtggctgagc atcacgtcag
    22501 ccagtacctg acttgcgggc ggtaactgtg ggacgacgat ggattgctcg gcgcgtagcc
    22561 cttgcgcatc gtaggtcacc agaaacaggc gaatgccgac cgacgacagc ccggcaaggg
    22621 tgattttctg atcgtcggcg ttaagcatca ctaacagcga ctgggttttg ccgttgaagc
    22681 tgccggtcaa cagttgctgc gaattgactg cgggtgaaat ccctggtgcc ggcagcgtga
    22741 tgcgcgtgcc aggttgcagc cacgcctgcg ggcggccttc ctgttctggt tgcgagtggc
    22801 tacagccggt gaggataagc gtagcggtca gggcgaacgc tcgccagaat gtggatttga
    22861 tcattttttt gttctctttt tatcgggcat agccagcggc gaaagcagga aggcggtgaa
    22921 aataccgctc accagcacaa tgccaaaact gctgatggcc tgggtggcgc tgaataccag
    22981 catacccagc gtcagcaagg tggtgagcat tgccagcgcg atcgccagta gcgaagttaa
    23041 cggtgtaccg cgcggattac tgaaaaacag cgtgtagttg ataccgatgc caagcaccag
    23101 caccagcgcc agcagcgaaa agagattcac cgcctgcccg ctcatcgcca gcaccgccag
    23161 accacagccc agcgaaagca ccgaaggcac caggctgata agccctttgc gccagccgag
    23221 acgggccact gcgccgcagg caatcactgc cagcgccacc agcaacaagc cggttaagac
    23281 gtagcggtaa agtgcgaaca attcatcaaa ggtgcttttg cgatcaaccc aggcaatgcc
    23341 gcaagggtaa tatgtggcga tttcctgcat caacgcgcta cttttaaccc cttcaaccgg
    23401 caccagtacg ccgctttcgc cgttttccag cgtcagccac agcagacgcc agccttcact
    23461 ggcggggctt gccagccatt catcaacgtt cactggcatg gcgttgagat ccgggttcac
    23521 tgccgtcagc ccggcatttt gcagcgcttt tgttactgtc ggggccgccg ttttcagcag
    23581 ttgtaaatct tcctcctgcc gcgcaaggga gttcagcgga atggtgcggt agttgctgat
    23641 aagcccctct tttttcgcat actcaagtga ggcggtatat ttctccagtc gccgcaatgt
    23701 ttgctgtggc gaatcgccgt aaaccacaaa ccatttttga tcgacgctct gcccggtcag
    23761 ggcggtaatg gctttttcct gcgccagaat atgctgcggt agcgcctgta actgcgagat
    23821 atcgtcatcg acgcgtagca ttgacatccc cgccagcgaa aacagcgcca gcgcgacggg
    23881 cagacccagc gacagttttt tattacgccg ccacgctgcc agccagcgta gcatcagcgc
    23941 catcgccgga accggacgca ccggcaggcc acggcacagc cacggatgcc agaacaggac
    24001 ggtcagacag gaggcgctca acccgacggc ggcaaaaatc gccatctggc gaatgccggg
    24061 gaagggggcg agcatcataa tcagatacgc cgccacggtg gtgagcagcg ccagtagcaa
    24121 ggcattgcgt actttcgcca ggctttgcca cggcgaaacg tcgttgccgt gaaccatccg
    24181 ctcggtgaga taatagagcg tgtagtcagc ggaaatgccg ataacgctca tgctcatcac
    24241 cagcgtcatc aggtgtaatt caccgaaaat caataaagtg gcgaccgttc ccgccagcgc
    24301 gccgatgccg atggaaatca cgcaaagcag caacgggcgt aaagagcgga acaccgccac
    24361 aatcagcaat atcaccccca gcagcgtagc cacgcccagg gtggagatat cctgcttcgc
    24421 ctgttggctg gcgtaatcgc tgtaaaacac cgtgccgcgc gagagcaact gtgcctgcgg
    24481 gtaacgcgtt ttcagatcct tttccagcgt attcagggtc gtgatcagct ggtgggtttg
    24541 ctgcatatca aacgacgatc ccgccagttc gccgtgcagc agataccagt agttgccctg
    24601 gggatcctgc gtcaccagcc aaccgtccat cagccgcaaa cgctggccgt ttttcgccat
    24661 tgccagctgc gagccgcgca ttaacatcag cggatcgttt tgcagctcct tgccgcttac
    24721 gccggagaat gcggaataaa gctgggagag gatccactgt gcctgcgctt cgccgccgtt
    24781 ttgcaggcgg gcgcgggtgt tggggtcaat caggccgttg cgatgctgcc agaaaaacgc
    24841 tccccacgcc tgctggctgg cggcatccat tggcccttta acgtcgccga gcgcagcgga
    24901 tttttgcagc agcgtcagcc actcctgagc gaccaaagga ttagcctctt taccggggct
    24961 gaccagccac accagttggc ggtcaagacg ctgcataaag ccgtcattca gcgccggagg
    25021 aatatcgccc atcgtctgtt tgggtaacat agccagcacg ctactgttca gccgtgattg
    25081 cggcagcagg atcagcaacg ccaccgccat gaccaggcag actagccccc ataacagcgc
    25141 ggggcgttta ctgggcggca aaacgttggc gttcgtcatc ggtcagttgc gctggcgtca
    25201 gttgatgttg ggtaagagca atatcggtgc gatcgccctg tttatcatta agttgaatgc
    25261 tctccagata ggttttcccg gcgagatcga tggtgttgaa aattttatcc agcggcgtgg
    25321 tggtcggcgt caggcgcagc gtccagcggc cttcgccttt gtcagcaaat tcgacgcgga
    25381 agttttgttc cagcactttg cgatcggcct ggaacaacgc gcgcagcagg tggttaaact
    25441 ggaacatctg cgggttgttt tctgccgtga tgatttgcgg cggctgaccg ttgatcacct
    25501 gcaccatgcg tttatcgtcc agtaatagtt gcatggggaa cggtgaggtt tgatcccaca
    25561 acagcccctg gtcgcgggcg atcaacatct ggccctgaga tcgcagcggc tgcggcagat
    25621 ctttaatcgt ccgggtttga tcgaaatggg cgcggatcac cggttgttcg gtaaagcgtt
    25681 gctgaagatc gtccagggtc agggcgctca caaacgggct aatcagcagc gccagcagcg
    25741 gtaaaaattt catggtttga ctcccatgcg ttcaaacaga atatcggggc tgacaaagca
    25801 cagttcgcga ctttgctcgt cgaccgcgac ctgaatggtg tatccggtgg tggcgcgttt
    25861 gcccgtttcg gcatcgaaaa tctgatagcc aatccgcaga cggttttcga actcttcgat
    25921 atgcgcacgc acgcggatgc gttgctcaaa ggtgagggcg tggcgatatt tcacccgcgc
    25981 atcgaccact ggccacaaat aaccggactc tttcatttgc cgatagccgt aattgaactg
    26041 gttgagcagc gcctcgcgag ccacttcaaa gtagcgaaaa tagttaccgt gccaggccac
    26101 gcccatcata tcgacgtcgt gaaagggaat ggtcagctcg acttctgcgg taaatcgggg
    26161 atcgttcagc accctttact ccttgtcctg aatttccggc agttgccaga aatcgaaaaa
    26221 attaaaccag tcgagaggcg actggagcgc gtaatgttcc agacgcgcgg cgtaatgatc
    26281 gatagcgttt tgcagcgcct gttggcgttc gccgcgcggc agctgcagtg ggtcggcaaa
    26341 gctttcgcag tgaatatgca gcttgccgtg ctggcgcagg gcgaaaatca gattcaccgg
    26401 gcagcgcaaa atagaggcga gaataaacgg cccctgtggg aaaggcgcag gctggcccat
    26461 aaacgaactc cagcagacgc gccagtcgcc gccgcgttgc gggttgacgg cgatgcggtc
    26521 accgacaatc gccacccatt cgccgttatc cagcttctct ttcagcagga tggcggtttc
    26581 tgggccgata tctgttaccg gcatcaggtt aatgcctgcc tgaggagcca tctcctgcat
    26641 tatctgttta aagcgttggg cgttttcgct aaacaccagc gcgttaatgg ttttgtagcc
    26701 ctgaattttt gccagcgccc ggcacacttc cacatcgcca agatgcgagg ccagcagcaa
    26761 tttgccgcgc ggatcgcgga cgtcaagcgc tgcttctgcg cctggcgcaa acagcacatc
    26821 acgccctggt tgtagctcgc cgcgccagct ggcgattttg tcgagcatgg cattaccgaa
    26881 acgtagaaag tgctgatagc tgttgagttt tgccgcctgt ggttgatgct gtcgtacacg
    26941 ggcgagccag tcttgcgacg ctttgcgcgc acgagaagca gtgagccagt agacgcccac
    27001 caccgggtaa agcagcgcgc taaacgccgt tcttcccagc agacgccaga ccagcagcat
    27061 caggcgcatt ccccataatc ctttcacttc actctgccgc gcccagtggc aggaagagcg
    27121 gcgcatcagc agtgaaggaa tacgcggcaa catgccgaaa aacagacgcg tgtgcatgag
    27181 cgagatgcgg acgttatctt tcagggcatc aaaatgcgaa agcccgtcca gtggataggt
    27241 gacgcgggtc ggcacgaaat agctggtatt tccctgccag tagaggcgca ccatcacttc
    27301 ggtgtcgaaa tccatccgct tgccgatggt ggcgtgtttt gccagttgca gcgttggcgc
    27361 aaccggataa acgcgaaaac cgcacatgct gtctttcagt tgcagggaga gcgtttcgat
    27421 ccatacccag acgtgggtga cccagcgccc gtaaagccgt gagcggggga tggagtcatc
    27481 gtaaattggc tggccggaga tcagtgccgc aggttgttgt tcagcgagag ccagcagttt
    27541 agggatatct tcaatcgcgt gctgaccgtc agcatccacc tgcaccgcat ggctgaaccc
    27601 tgcgtctgcc gctgcctgta agccacgcat caccgccgcg cctttgcctg cgttttctgc
    27661 caggcgaatt aaggtcacgc caggctgttc ggcaagcaga ctgtccagtt gctgttgtgt
    27721 ggtggcatcg ctgccgtcat ccaccacaat acagggcaga ttaaatggct taagacgcgc
    27781 cagcacgccc ggcatcatcg cgccgtggtt gtagcagggt atcaacacgc agggagaaaa
    27841 gtttaccgac ataggcggat cttcccgcta ctggcggtgt ggcgggcgtc accgtcgtga
    27901 cgctgatagc taaagctcag aatctggcgt tcttcctgcc agttaagcgc gagcgtgacg
    27961 cggttttccg gcagcaacgg agactggaat tttatgttct gaatggagtg aaaacgccag
    28021 ccaggcgcga gcagattgag cgcgtagctc atcgcccagt cgatttgcgc aactcctggg
    28081 agtaatggtt gcacggcaaa atgaccgcga aaccagaaca gatccgccct gagatgcaga
    28141 acaatttcaa ggtgattcgt ttgtgcctga tggcgctcga tttcatgggg cttcatgaaa
    28201 taactcctgt aattgcgcat agacacgctt gttcatactg tttactggca tttcatcaat
    28261 aacgcgccag taacgcggaa tagcaactgg ctccagcgtc gggcgcagta aacgccgcca
    28321 ggtcatttcc tggctatgcc cgccgccgtt ctgccattgc agatgagctt cgtcatccag
    28381 caccagcaaa acgcctatgc tctgacgccc gccgcgcgta acgggaactg ccgccgcctc
    28441 gcgaatgccg tccagcgcca gtagacgttg ttctacttcc tggagtgaaa tacgtttttc
    28501 ttcaatctta acgatgcgcc cacgacgccc catcagatgg aactgaccat cttcgctaaa
    28561 ctgtaagata tcgtcgagta acatgccgtt atcttccgct atcagcggtg aaaagaggcg
    28621 aaaggcatcg ccttcggcct ggaactgcat acctgggagg ggtagccagc ggcgttgctc
    28681 ttgttcgcgg tagcgccagg cgatgacgcc agtttccgtg ctgccgtaga tttcatcagg
    28741 ccagacgcgc agccagctcg cggtatgttg tacatcctgc cacggcagct caccgccagc
    28801 agagagaagc acctgaacgg gtggcggaga aagctgggta tccaggcgct tcaaaaatgc
    28861 cgggctgctg atgaagatat agcgatgctg atgactcaac gcggcaaact gttcgacata
    28921 ccagagcatg gcggcgtgca gcggtaatcc caacgccatg ggcaggaaaa cgcgaaaagt
    28981 caggccgtac aaatgctgcg gcaatacgga accgacgaca cgacaacccg ccagacgtgc
    29041 gcctaatcgt tcagccagca gttctgcttc acgatccagc aaatgaacag gtttaatcgc
    29101 ccgtttcggc tgtcccgtgg aaccggaggt aaacagctcg ataaaagcct ctggcgctat
    29161 agcggcaaag gtgaaggatt gtgtcgcgat ttgtggagaa cttgctacca acagcaatga
    29221 accttgccag ttaagttcgc tgtcgctaag cacaccatca aaaagttcac gttgctcgtt
    29281 aagctggata acacggttat gccccggaag caccggcgtt tttccggcgt gtagcgtggc
    29341 gagcagggca acgataaaca gatagctgtt ctcaaagcac agcgcccagc gttcacccgg
    29401 ctgttgctgc aagcggcaga taagttgcgc gacatcgtga cgcaaatcgc ccagcgtcca
    29461 ggtactttcg ttcagccacg caatgggtgt gtcatcaggg cgtggcgcag tcagccagcg
    29521 tgcaagcggt aaggtctgct tcatgcgcga tctcttttca tcacctgacg gcgcagcagc
    29581 cattcaccag ccattaacgt ccccatcaac aaataagcaa tcatgccgtt ccaggcggtc
    29641 cataacgaca tatcggcata aagcgcggta aagagggcaa ttccaccgtt aatgatgaaa
    29701 aagccgcacc agatttgcgt gacgtggcgg gtatagcgca cgcccttttc cgggaggtct
    29761 ggctcctgca accgcgccag ccgctcaact atcggcattg ccgaccacag cgaaccgcca
    29821 aaaaccgcca gcatgaccgc gttaaccact accgggtaaa acagcagtag ctggtgcgtt
    29881 ttcaacataa agctggcgac acataatgcg atgcccacga cagcgacgag ttgcgtgacg
    29941 gcttgtaaag gccccgcctg gcgacgggtc tggcgcagac gcaacaaaag cagcaacgcc
    30001 attagcggta acagccagtg caggccgtta tgcgctaagc caaaccaaat cacaaacggc
    30061 caggcgagca gcaataatcc agtcagcagc ttaatcatcg gtagcgagcg aatgcccgac
    30121 atcagatcag gcttcttgta gcaggcgttc tacagcctcg acgacatcct ggactgtacg
    30181 cactgcttta aactcttccg gcttgatttt cttaccggtt ttcttttgca ggtgcacaat
    30241 catgtcaacg gcgtcgatgc tgtccagttc cagatcttcg tacaggcgcg tttcaggttt
    30301 gatgtcctgc ggatcgattt caaacagctt aaccagcagg gctgagactt cctgatagac
    30361 ggtttgttgt tctgtcatga tggctttcct caggcacgtt gcgcagcaat gaaagaggcc
    30421 agagtggcga cggagaaaaa gtgctgacgc atctcttcgc tttccgcaga gagcacgatg
    30481 ccgtactcat tttttactgc cagtcccagt tccagggcat cgatagagtc cagccctaaa
    30541 ccgtcgccaa acagcggcgc gtcggtatcg atatcatcag gcgtcaactc gtccagattg
    30601 agggtgctga tgatgaggtt tttaatttcc agataaagcg cttgcattat ttattcctga
    30661 gagaggtaaa caaccttgtt gtaattgaag caaaagatgc cggtttaact gccttgctgc
    30721 cagcgccggt tcttgtgaag ttgcatcgta aaatttattg atttcaaccc gaccatggac
    30781 tgcaaccgta aaaaacggcc ggtcaggtgg aacgtcatac catttacttt gtttatccag
    30841 cagatgctcg ctacagccga ttgtcacgat gcgtaaatcg ctggcgcagc gcacggcaat
    30901 attcgccgcc ccgcgctgca gcgtcatttt ttcaccgggc ttcgttcgtg tgccttcggg
    30961 gaaaatcagg atagtgtcac cctgtgccag tcgctgttgg cagcggggta acagagtttc
    31021 agcttcgctg ttgattaaat aatctgtcgc ccgcacgacg ccgccaagaa aaggattttt
    31081 taacagcgcg cttttcacca ggcagtcagt ttctggcatg actgacgcca gcagcacgta
    31141 atcgatcagc gtgggatgat tggctacgac gaggcaaccg cgttcctgat gtaagatttc
    31201 agcgccatca atacgataat ccagtacgcc aagccctttc gcgacagtta aaaacaggcg
    31261 aaaactggcg gcaatgctgc gacgcgccaa acgacggcgg cgggaagtat cccacaccaa
    31321 aactaacaat atgttaaacc acacgacaga aagcagcagg ccgccgagac caaacagggc
    31381 aaaacataac ccggtcatga ccagtcgcca ggtccactcc agacgtgaca tcagcttact
    31441 catctgtggc tccagcgcca ttgcacgcgc tcgccaggca gtgaaaatgt gtctacattt
    31501 tgtaaatagt gctgtaaaaa caacatgctt tgcggcagtg ttgtttcatt gcccgcaatg
    31561 gctgactgcg tttcacattg ccagtcatcg ccagcttcaa tcaccagcgc gacggcgtac
    31621 ggccaggtcg gcatttctga tggcagttgt gggtgataaa actcgggcag gaaaccgtca
    31681 aaatcgacca tcagcacgcg ctggtatccc gcttgtagca ggctaagcac ttcacacaaa
    31741 ccttgctgga aactatcgcg accagccgaa agcgatgacg aaacgctcgg ctgtttggcc
    31801 gcaatggtca ggttacccac ggaagagttg tgtacggaga gcgcgaagtc cgtcggagaa
    31861 agcgcctgtt cggttgccag tgcatgaaca atgcggtaat tgcgctccag ttcgccatga
    31921 cgactggtat acaaaacggc gtctggttga tggcggcgta gcattgccag accgcattca
    31981 acagccagtt tgctcccgga gctgaggcgg cgggcggtca tcattggcag ttcacttaat
    32041 tttgccaacg gtgctgtcgg gtcaattgcc tgaggctgac gtgaccactc ctgccattgc
    32101 agcgtttcac taagtccagg cgctcttgcc tgccagtcga gaatgtttaa tgcaaatttc
    32161 atcaacgcgc gcccgcggta aaccaaaatt attaagtgac gattatttct tttggcatat
    32221 caaaagtgta tgcccaacgc ctaaattatc gtggcgctct gctacctgga atcctgcttt
    32281 attcagatag tcgtaaaatt tctctgcact atagaaacgg ctgttaccgt ttgccatgca
    32341 ggtaaaataa agtgaggacg cgttcaggct aaacgatgct gcttcaaacc gctgcgcatc
    32401 ccagaacaac tccatgatgc aaacttttgc gccaggcttc attacgctgg caattttgct
    32461 gagtatgcta attatttgtt cgggtgaaaa acagtctaaa aattggctca tccaccagat
    32521 atcagcctcg cccggtaatg gggcatcgct tagcatatcc accgcatgga aatctatgcg
    32581 atcagaaaaa cctgcattcg cgatattttc tttcgctagt acgatttgtt gtggcagatc
    32641 taataacgta acggcgatgt tttcattgta tttgcagcaa cgcaaagccc acttacccgt
    32701 attgccgcct acatcgtaca atgttgtggg gttgttggcg aatacataag gcagtgcggc
    32761 gttaaatgcg ccatctgaat agtaatgatc aaacgcaaac cagctttcac gagcggcgtc
    32821 tggtaattgc gaaagtgccg gatatatcgt cgaccagtcg ccaaatactt ttaacccgga
    32881 aggttttcct tcattgagtg aatcggcgag gaagaagagc ccctggtaac agacatcctg
    32941 ggtgaaatcc atattcacgc gagtcatagt gtcatgcagg aggtaatgac cgattttcgc
    33001 caggtaataa attccctctt tacaggtaat aattcgcccg cttaagccca tatctaacag
    33061 aacgctggta gcatattcgt tgatatggct atgttcggtg atatcagcca gcgttgcgcc
    33121 atgtttgcct tgccgatcca gatatgaaag tacacccgca ttacgtaggc agagtgctgt
    33181 ttgaaaaagc ataggggcaa aggcaatgcg ttgagcttcg gtaatcgcat ccagtgcgct
    33241 aagagaatcc tgttcgtaca tgttgggcac aacctgttaa gagggaatta aattattatc
    33301 ggaccgcaac ggcccgactt taggaagaat tataaatctg cgcccgctga caaattcagt
    33361 tgaatatctt tgtcgagatt gcggacccag cgattgtagt ttttatgaat ttgtccgcct
    33421 gacgcttgta ggttctggct attttcataa ttgatatcgt agctggatgc cgtgtagtta
    33481 atgcgtattt ccgctgaatg atcgcgtgac tgttgacgtg ctttaattat gccttgtccg
    33541 gcttcggaca taatccattt gcgttcaacg ccagctttca gaatcgcttt tttcacttgt
    33601 tcctgggtat gaccagcgct gacggttgag tgaacctgat caattggcgc agtacgcgcg
    33661 caacctgcta aagcacccac aacagcaata gccgcaaacc atttcatgta atgtctcatt
    33721 agaaccccat taaagtattg tttttacgag taaagcaagt tttaactgta attgctggca
    33781 tcactattct ttatttagag aaaaaattac ttgtgagata agtcagtaag tgcacatcag
    33841 agtaaaaatc tctgaaatga ctaaattact gccagcaggg accaactata agtgagtgag
    33901 aaaaatgagg caataaaaat aacacaatgt tatcagtgca ttct
//
LOCUS       DAZKIF010000041        33788 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZKIF010000041 DAZKIF010000000
VERSION     DAZKIF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.041"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..868
                     /gene="cynX"
                     /locus_tag="ACHOTW_001950"
     CDS             <1..868
                     /gene="cynX"
                     /locus_tag="ACHOTW_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP9844064.1"
                     /translation="LGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGA
                     ALTPWLVQHSETWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRA
                     WTLGVYFGLINGGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMAR
                     HQDRRKLLMLALALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQ
                     PAIAGKLVAFMQGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFA
                     PARFPQLWVKEA"
     gene            complement(971..1582)
                     /gene="lacA"
                     /locus_tag="ACHOTW_001951"
     CDS             complement(971..1582)
                     /gene="lacA"
                     /locus_tag="ACHOTW_001951"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFP9844065.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(1648..2901)
                     /gene="lacY"
                     /locus_tag="ACHOTW_001952"
     CDS             complement(1648..2901)
                     /gene="lacY"
                     /locus_tag="ACHOTW_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP9844066.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(2953..6027)
                     /gene="lacZ"
                     /locus_tag="ACHOTW_001953"
     CDS             complement(2953..6027)
                     /gene="lacZ"
                     /locus_tag="ACHOTW_001953"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP9844067.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(6150..7232)
                     /gene="lacI"
                     /locus_tag="ACHOTW_001954"
     CDS             complement(6150..7232)
                     /gene="lacI"
                     /locus_tag="ACHOTW_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP9844068.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(7269..8222)
                     /locus_tag="ACHOTW_001955"
     CDS             complement(7269..8222)
                     /locus_tag="ACHOTW_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFP9844069.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(8242..9018)
                     /locus_tag="ACHOTW_001956"
     CDS             complement(8242..9018)
                     /locus_tag="ACHOTW_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9844070.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(9133..9966)
                     /gene="mhpR"
                     /locus_tag="ACHOTW_001957"
     CDS             complement(9133..9966)
                     /gene="mhpR"
                     /locus_tag="ACHOTW_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP9844071.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            10157..11821
                     /gene="mhpA"
                     /locus_tag="ACHOTW_001958"
     CDS             10157..11821
                     /gene="mhpA"
                     /locus_tag="ACHOTW_001958"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP9844072.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            11823..12767
                     /gene="mhpB"
                     /locus_tag="ACHOTW_001959"
     CDS             11823..12767
                     /gene="mhpB"
                     /locus_tag="ACHOTW_001959"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP9844073.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            12785..13651
                     /gene="mhpC"
                     /locus_tag="ACHOTW_001960"
     CDS             12785..13651
                     /gene="mhpC"
                     /locus_tag="ACHOTW_001960"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP9844074.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            13661..14470
                     /gene="mhpD"
                     /locus_tag="ACHOTW_001961"
     CDS             13661..14470
                     /gene="mhpD"
                     /locus_tag="ACHOTW_001961"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP9844075.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            14467..15417
                     /gene="mhpF"
                     /locus_tag="ACHOTW_001962"
     CDS             14467..15417
                     /gene="mhpF"
                     /locus_tag="ACHOTW_001962"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP9844076.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            15414..16414
                     /gene="mhpE"
                     /locus_tag="ACHOTW_001963"
                     /pseudo
     CDS             15414..16414
                     /gene="mhpE"
                     /locus_tag="ACHOTW_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
     gene            16590..17801
                     /gene="mhpT"
                     /locus_tag="ACHOTW_001964"
     CDS             16590..17801
                     /gene="mhpT"
                     /locus_tag="ACHOTW_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP9844077.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            17903..18442
                     /gene="yaiL"
                     /locus_tag="ACHOTW_001965"
     CDS             17903..18442
                     /gene="yaiL"
                     /locus_tag="ACHOTW_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP9844078.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDNPYADFKVPDDLMW"
     gene            complement(18567..19400)
                     /gene="fghA"
                     /locus_tag="ACHOTW_001966"
     CDS             complement(18567..19400)
                     /gene="fghA"
                     /locus_tag="ACHOTW_001966"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9844079.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYHPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(19493..20602)
                     /gene="frmA"
                     /locus_tag="ACHOTW_001967"
     CDS             complement(19493..20602)
                     /gene="frmA"
                     /locus_tag="ACHOTW_001967"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP9844080.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(20637..20912)
                     /gene="frmR"
                     /locus_tag="ACHOTW_001968"
     CDS             complement(20637..20912)
                     /gene="frmR"
                     /locus_tag="ACHOTW_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP9844081.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(21072..22118)
                     /gene="fbpC"
                     /locus_tag="ACHOTW_001969"
     CDS             complement(21072..22118)
                     /gene="fbpC"
                     /locus_tag="ACHOTW_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFP9844082.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(22130..24208)
                     /locus_tag="ACHOTW_001970"
     CDS             complement(22130..24208)
                     /locus_tag="ACHOTW_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9844083.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(24277..25308)
                     /locus_tag="ACHOTW_001971"
     CDS             complement(24277..25308)
                     /locus_tag="ACHOTW_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9844084.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(25305..26621)
                     /gene="uhpC"
                     /locus_tag="ACHOTW_001972"
     CDS             complement(25305..26621)
                     /gene="uhpC"
                     /locus_tag="ACHOTW_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9844085.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(26694..28235)
                     /locus_tag="ACHOTW_001973"
     CDS             complement(26694..28235)
                     /locus_tag="ACHOTW_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9844086.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(28235..28864)
                     /locus_tag="ACHOTW_001974"
     CDS             complement(28235..28864)
                     /locus_tag="ACHOTW_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9844087.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRLVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            29167..30129
                     /gene="tauA"
                     /locus_tag="ACHOTW_001975"
     CDS             29167..30129
                     /gene="tauA"
                     /locus_tag="ACHOTW_001975"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9844088.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFSKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            30142..30909
                     /gene="tauB"
                     /locus_tag="ACHOTW_001976"
     CDS             30142..30909
                     /gene="tauB"
                     /locus_tag="ACHOTW_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP9844089.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            30906..31733
                     /gene="tauC"
                     /locus_tag="ACHOTW_001977"
     CDS             30906..31733
                     /gene="tauC"
                     /locus_tag="ACHOTW_001977"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP9844090.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            31730..32581
                     /gene="tauD"
                     /locus_tag="ACHOTW_001978"
     CDS             31730..32581
                     /gene="tauD"
                     /locus_tag="ACHOTW_001978"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP9844091.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(32688..33662)
                     /gene="hemB"
                     /locus_tag="ACHOTW_001979"
     CDS             complement(32688..33662)
                     /gene="hemB"
                     /locus_tag="ACHOTW_001979"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP9844092.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         7855 a         8896 c         9289 g         7748 t
ORIGIN      
        1 gctcggtggg gtggggatcg gcatcattca ggcggtgatg ccttcggtga ttaaacggcg
       61 gtttcagcag cgcacgccac tggtgatggg gctgtggtcc gcggctctga tgggcggcgg
      121 tgggcttggt gccgccctga caccctggtt agttcaacat agcgaaacct ggtatcaaac
      181 actcgcctgg tgggcgctgc ctgccgttgt tgcgctcttt gcctggtggt ggcaaagcac
      241 ccgcgaggtc gcctcttccc acaagacaac aaccattccg gttcgcgtgg ttttcactcc
      301 tcgcgcgtgg acgctgggtg tttacttcgg tctgattaac ggtggttacg ccagcctgat
      361 tgcctggtta cccgccttct atattgagat tggtgccagc gcgcagtaca gcggttcctt
      421 gctggcattg atgacgcttg ggcaagccgc aggagctttg ctgatgcctg ctatggctcg
      481 ccatcaggat cggcgcaaac tgttaatgct ggcgctggcg ttacaactgg tgggtttctg
      541 cggctttatc tggctgccgc tgcaattgcc ggtattgtgg gcgatggtgt gtggtttagg
      601 tctgggcggc gcatttccgc tctgtttgct gctggcgctc gatcactctg tgcaaccggc
      661 tattgctggc aagctggtgg cgtttatgca gggaatcggt tttatcatcg ccgggcttgc
      721 cccgtggttt tctggcgtgc tgcgtagtat cagcggcaat tacttgatgg actgggcgtt
      781 tcatgcgctg tgcgtcgtag ggctgatgat tataaccctg cgttttgcac cagcacgttt
      841 tccgcagctg tgggtcaaag aggcatgatg cgacgcttgt ttctgcgctt tgttcacgcc
      901 ggatgcggcc tacatagatc actgaatttg taggcctgat aagcgaagcg tatcaggcaa
      961 tttttataat ttaaactgac gattcaactt tgtaatctct gaaataatat tgcttatccc
     1021 ggtcgtttat ttcgcgaata acccgacaag gaacgccagc cgccacgacg tttggtggaa
     1081 tgtcttttgt gacgatacta cccgcgccaa taacagaatt atccccaatg gtgacgcctg
     1141 gattaataac cacatgactt ccgatccaga cgttattgcc aatcgttatc ggaaaagagt
     1201 acatctcacc gttttttctc aattcatggt gtacagggtg tcccgtaacg gaaagagtaa
     1261 cgttgggtgc aatcaatacg ttatcaccga ttgttaccgt gtagtcatcg acaatggtta
     1321 aattgaaatt tgcataaaaa ttgcggccta tatggatgtt ggaaccgtaa gagaaataga
     1381 cggggggttc tacccaggcg ttttccccta ccgtggcaaa catttcttta atcaggcttt
     1441 ctcttttatc aacttctgat ggatgcgagt gattaaactc atacattaac gtttttctac
     1501 gaagtctttt ttcaggtaag ccttcgcaca tatcggtaaa tagcttgcct gcttttattc
     1561 tttctgtcat cgacatgttc attgcgatca ttccgttatg atatgttggt cggataaggc
     1621 gctcgcgccg catccgacat tgattgctta agcgacttca ttcacctgac gacgcagtag
     1681 agaaagcggg ccggggccgc taagcgtgaa cacggaaatt aaggtgaagc ccagcgccac
     1741 cagacccagc accagataag cgccctggaa accgatgctt tcatacatat tacccgctag
     1801 tacagacata aaaatcatcg ccagttgctt aaagaagcag aaacagacca gataaatcgt
     1861 cgctgaaaaa cgcacttcaa actggctggt aatatattta aagcagccca ccagcaggaa
     1921 cggtacttca aacatatgca gcgttttcag aataaccact tccagcgctg aggtggcgaa
     1981 cgatgagcca ataatacgta cagacataat agtgccagcc agcagcaggg catttttccc
     2041 accgatgcga ttaatgatca gtggcgcaaa gaacataatt gaggcgttaa gtaattcgcc
     2101 cattgtcgtt acgtagccaa atacccgcgt accctgttca ccggtggcaa agaaagaagt
     2161 aaagaaatta gcaaactgtt ggtcaaaaac atcgtaggtg caggaaacgc caataacata
     2221 cagtgacaaa aaccacagtt ttggctgtct gaacagttcc agcgccagtt taaggctaaa
     2281 tgccgaatgg ttggcaccta ccgcattggc aaccgtggcg gaagagggcg catccgtttt
     2341 ggcgaaaaag agtaaaatgg cgaggatgag tgcacagcca gaacccagcc agaaaacgaa
     2401 ctgattattg atggtgaaca tgatgccgac aatcgaggca cacagcgccc agccaacaca
     2461 gccaaacatc cgcgcgcgac caaattcgaa attactgcga cggctgactt tctcgataaa
     2521 tgcctctact gcgggcgcac cggcgttaaa acaaaagcca agataaatac caccaacaat
     2581 cgatcctact aaaatgttgt attgtaacag tggcccgaag ataaaaataa agaacggcgc
     2641 aaacatcact aacatgccgg taataatcca cagcaggtat ttgcgcagcc cgagtttgtc
     2701 agaaagcaga ccaaacagcg gttggaataa tagcgagaac agagaaatag cagcaaaaat
     2761 aatacccgta tcacttttgc tgatatggtt gatgtcatgt agccaaatcg ggaaaaacgg
     2821 gaagtaggct cccatgataa aaaagtaaaa gaaaaagaat aaaccgaaca tccaaaagtt
     2881 tgtgtttttt aaatagtaca taatggattt ccttacgcga aatacgggca gacatggcct
     2941 gcccggttat tattattttt gacaccagac caactggtaa tggtagcgac cggcgctcag
     3001 ctggaattcc gccgatactg acgggctcca ggagtcgtcg ccaccaatcc ccatatggaa
     3061 accgtcgata ttcagccatg ttccttcttc cgcgtgcagc agatggcgat ggctggtttc
     3121 catcagttgt tgttggctgt agcggctgat gttgaactgg aagtcgccgc gccactggtg
     3181 tgggccataa ttcaattcgc gcgtcccgca gcgcagaccg ttttcgctcg ggaagacgta
     3241 cggggtatac atgtctgaca atggcagatc ccagcggtca aaacaggcgg cagtaaggcg
     3301 gtcgggatag ttttcttgcg gccctaatcc gagccagttt acccgctctg ctacctgcgc
     3361 cagctggcag gtcaggccaa tccgtgccgg atgcggtgta tcgctcgcta cttcaacatc
     3421 aacggtaatc gccatttgac cactaccatc aatccggtag gttttccggc taataaataa
     3481 ggtttttccc tgatgctgcc atgcgtggac agtggtaatc agcaccgcgt cggcaagtgt
     3541 atctgccgtg cactgcaaca acgccgcttc tgcctggtaa tggcccgcag ccttccagcg
     3601 ttcgacccag gcgttagggt caatgcgggt cgcttcactt acgccaatgt cgttatccag
     3661 cggtgcgcgg gtgaactgat cgcgcagcgg cgtcagcagt tgttttttat cgccaatcca
     3721 catctgtgaa agaaagcctg actggcggtt aaattgccaa cgcttattat ccagctcgat
     3781 gcaaaaatcc gtttcgctgg tggtcagttg cgggatggcg tggggcgcgg aggggagtgt
     3841 cacgctgagg ttttccgcca gacgccactg ctgccaggcg ctgatgtgtc cggcttctga
     3901 ccatgcggtc gcgttcggtt gcactacgtg taccgttagc cagagttgcc cggtgctctc
     3961 cagtcgcggt agttcaggca attcaattac ctgtttacct tgcggagcga catccagagg
     4021 cacttcaccg cttgccagcg gcttaccatc cagcgccacc gtccagtgca ggagctcgtt
     4081 atcgctatga cggaacaggt attcgctggt cacttcgatg gttcgcccgg ataaacggaa
     4141 ctggaaaaac tgctgctggt gttttgcttc cgtcagcgcc ggatgcggcg tgcggtcggc
     4201 aaagaccaga ccgttcatgc agaactggcg atcattcggc gtatcgccaa aatcaccgcc
     4261 gtaagccgac cacggattgc cattttcatc atatttaatc agcgactgat ccaccaagtc
     4321 ccagacgaag ccgccctgta aacgggggta ctgacgaaac gcctgccagt atttagcgaa
     4381 gccgccaaga ctgttaccca tcgcgtgggc gtattcgcaa aggatcagtg ggcgcatttc
     4441 tccaggcagc gaaagccatt ttttgatgga ccatttcggc acagccggga agggctggtc
     4501 ttcatccacg cgcgcgtaca tcgggcaaat aatatcggtt gcggaggtgt cggctccgcc
     4561 gccttcatac tgcaccgggc gggatggatc gacagattta atccagcgat agagtgcgtc
     4621 gtgattagcg ccgtggcctg actcattccc cagcgaccag atgatcacac tcgggtgatt
     4681 acgatcgcgc tgtaccattc gtgttactcg ttcgctcatg gccggtaacc agcgcggatc
     4741 atcggtcaga cgattcattg gcaccatgcc gtgggtttca atattggctt catccaccac
     4801 atacaggccg tagcggtcgc acagggtgta ccacagcgga tggttcggat aatgcgaaca
     4861 gcgcacggcg ttaaagttgt tctgcttcat tagcaggata tcctgcacca tcgtctgctc
     4921 atccatgacc tgaccatgca gaggatgatg ctcgtgacgg ttaacgccgc gaatcagcaa
     4981 cggcttgccg ttcagcagca gcagaccatt ttcaatccgc acctcgcgga aaccgacatc
     5041 gcaggcttct gcttcaatca gcgtgccgtc ggcggtgtgc agttcaacca ccgcacgata
     5101 gatattcggg atttcggcgc tccacagttt cgggttttcg acgttcagac ctagtgtgac
     5161 gcgatcggca taaccgccac gctcatcgat aatttcaccg ccgaaaggcg cggtgccgct
     5221 ggcgacctgc gtttcaccct gccacaaaga aaccgtcacc cgcagctcat cgcgcagctc
     5281 gccgtacatc tgaacttctg cctccagtac cgcgcggcta aaatcatcat taaagagagt
     5341 ggcaacatgg aaatcgctga tttgtgtggt cggtttgtgc agcaacgaga cgtcacggaa
     5401 aatgccgctc atccgccaca tatcctgatc ttccagataa ctgccgtcac tccagcgcag
     5461 caccatcacc gcgaggcggt tttctccggc gcgtaaaaat gcgctcaggt caaattcaga
     5521 cagcaaacga ctgtcctggc cgtaaccgac ccagcgcccg ttgcaccaca gatgaaacgc
     5581 cgagttaacg ccatcaaaaa taattcgcgt ctggccttcc tgtagccagc tttcatcaac
     5641 attaaatgtg agcgagtaac aacccgtcgg attctccgtg ggaacaaatg gcggattgac
     5701 cgtaatggga tatgtcacgt tggtgtagat gggcgcgtcg taaccgtgca tctgccagtt
     5761 tgaggggacg acgacagtat cggcgtcagg aagatcgcac tccagccagc tttccggaac
     5821 cgcttctggt gccggaaacc agacaaattg ccactcacca ttcaggctgc gcaactgctg
     5881 ggaagggcga ttggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     5941 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaatacgac
     6001 ggccagtgaa tctgtaatca tagtcatagc tgtatcctgt gtgaaattgt tatccgctca
     6061 caatttcaca caacatacga gccggacgca tagagtgtaa agcgtggggt gcctaatgag
     6121 tgagctgact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacttgt
     6181 cgtgccagct gcattaatga atcggccaac gcgcggggag aagcggtttg cgtcttgggc
     6241 gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc
     6301 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc
     6361 tgtttgatgg tggttaacgg cgggatataa cacgagctgt cttcggtatc gtcgtatccc
     6421 actaccgaga tatccgcacc aacgcgcaac ccggactcgg taatggcgcg cattgcgccc
     6481 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc atttagcatt
     6541 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cctcccgttc cgctatcggc
     6601 tgaatttgat tgcgagtgag atatttatgc cagcccgcca gacgcagacg cgccgagaca
     6661 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc
     6721 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg agtctggtca
     6781 gagacatcaa gaaataacgc cggaacatta gcgcaggcag cttccacagc aatggcatcc
     6841 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc
     6901 actgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca
     6961 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc
     7021 agactggagg tggcaacgcc aatcagcaac gactgtttcc ccgccagttg ttgtgccacg
     7081 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca
     7141 gaaacgtggc tggcctgatt caccacgcgg gaaacggtct gataagagac accggcatac
     7201 tctgcgacat cgtatagcgt tactggtttc acattcacca ccctgaattg actctcttcc
     7261 gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cggcggggct gacgccgaag
     7321 tgagatttaa aatgctgacg ccaggtcaca ggtgagagaa aacccacctc agccgctatc
     7381 cgctcaacgg gcaactgact ggatcccaac agttcctggc tacggcgtaa gcgttcagta
     7441 atgagccatt cggcgatact cgaaccggtc gctttcataa aatggcgagt taatgtgcgg
     7501 cgactcatca ttactcgctg cgccagcgag tcgagatcat gctgttgcgc aatgttttgc
     7561 cgcaggtaat ccagcaggag gtttatgcgt tcatcatggg tatttttcgg caccggttgc
     7621 tcaataaact gcgcctgacc gccggtgcga tatggcggta cgaccatccg tcgggcaata
     7681 tggttagcat agtcgctgcc aaaatgttgc cgaacaatat acaaacagca atccagcgcc
     7741 gcggcagtac cggcagaggt aataatgccg tcatcgtcta cgtacaacgc gttaatatcc
     7801 agatgtgttt gtggaaaacg tgcctgaaat tcacgctcga actcccagtg cgtggcggca
     7861 cgcttatttt tcagtaaacc tgcatagccg agcacaaacg tgcccaggca aagcccgaca
     7921 atctgtgcgc cgttatcccg tgcggtcgtt aaggcttcca gcagtttttg tggcggtttt
     7981 tgtgtaatgg ttccccagta aggaattatc acaatatctg cctggatgac tgcggtatag
     8041 tcatgggtgg cggttacgga aaacccatcg gcagagtcca cgtttcccgg cttttcagcg
     8101 caaataatca cgtgaaagcg ttttttctcc gccatctttt cactaaacac gataaacggt
     8161 acggaaaggt gaaaaggact aaaaccggca acggcgataa tggcaacagt tagcgggcgc
     8221 ataacactct ttatctccga attagaatgt caacgtttca ccatcttctg gtacgttcac
     8281 aaattcgata atttgattat ctctggcgta ttccttgagc gcatcacgcg tcagcaaaca
     8341 gtggtttacg gcctccatat gtgtcgcgat aatttgcgct tctgggaggg taaaatgtgt
     8401 tttgagaata tcttctgctc ccataattat cgcgccatgc cccagaacat gcgcaaatcc
     8461 ggcattcaac acaaccactt ctggttggaa agttagcatt gtagcctcga cgtcgtcacg
     8521 ccagagggta tcgccagcca gatagagcgt tttctctgtc gggtgctgga aaacaacgcc
     8581 gcaagctgcg cctaaacgct tcgccatcgc cgggtcggca taggtgcgat cggagccgtg
     8641 ctgccctgtc gttttatgca gagtaatatc gccaaattta gtctcgtcgg taagtacggt
     8701 aagatgcgta aatccttgtt tattcagtga ttcagcatct tgctcatttt gcacataaat
     8761 cagtttatct ttagggacga cgcgggaggc tgtttcgtcc cagtgatctt catgcaaatg
     8821 ggtcacgatt agggcgtcaa cgttgagtaa cgttgttaaa tcgcacggta attctaccgt
     8881 tggattgcgg atttctgcgc gtgccgttcc ggcaaaaccc ggataacttc ctttaggcgc
     8941 gagtaacggg tcgataagaa atgttttgcc agcgtattgg ataatttggg tggcattacg
     9001 aatttgcgta attttcatga tttgctccac atcacagtat tgatgtcatt agcgtagcgt
     9061 gcagcccgta ctgtgcatgt gggcaaatgg gcgaataacg atgagaatgg gccaaattac
     9121 acattggcgt aactagcgta aatgcacgcc gcttcgccgt ccggccacca gaatagcctg
     9181 cgattcaacc ccttcttcga tctgttttgc tacccgttgt agtgccggaa gatgcttttc
     9241 cgctgcctgt tcgatggtca ttgcgctcgc catatacacc agattcagac agccaatcac
     9301 ccgttgttca ctgcgcagcg gtacggcgat agaggcgatc ttctcctcct gatcccagcc
     9361 gcggtagttc tgtccgtaac cctctttgcg cgcgcgcgcc agaatggctt ccagctttaa
     9421 cggttctcgt gccagttgat agtcatcacc ggggcgggag gctaacatct cgattaattc
     9481 cttgcgttct tgttccgggc aaaaggccag ccaggtcagg cccgaggcgg ttttcagaag
     9541 cggtaaacgc cgcccgacca ttgcccggtg aaaggataag cggctgaaac ggtgagtggt
     9601 ttcgcgtacc accattgcat caacatccag cgtggacaca tctgtcggcc ataccacttc
     9661 gcgcaacaga tcgcccagca gtggggccgc cagtgcagaa atccactgtt cgtcacgaaa
     9721 tccttcgctt aattgccgca ctttgatggt cagacgaaaa ctatcatcgg agggactacg
     9781 ccggacatat ccctcttcct gcagcgtctc cagcagtcgc cgcacagtgg tgcgatgcag
     9841 gccgctgagt tccgccagca gcccgacgct ggcaccgccg tcaagtttat ttaacatatt
     9901 taataacatt agaccgcggg ttaagccgcg cacggttttg tattccgtct gctcattgtt
     9961 ctgcatatta attgacattt ctatagttaa aacaacgttg tgcacctggt gcacattcgg
    10021 gcatgttttg attgtagccg aaaacaccct ttctatactg agcgcacaat aaaaaatcat
    10081 ttacatattt ttaacaaaat aagttgcgct gtactgtgcg cgcaacggca ttttgtccga
    10141 gtcgtgaggt actgaaatgg caatacaaca ccctgacatc cagcctgctg ttaaccatag
    10201 cgttcaggtg gcgatcgctg gtgccggtcc ggtcgggctg atgatggcga actatctcgg
    10261 ccagatgggc attgacgtgc tggtggtgga gaaactcgat aagttgatcg actacccgcg
    10321 tgcgattggt attgatgacg aggcgctgcg caccatgcag tcggtcggcc tggtcgataa
    10381 tgttctgccg catactacgc cgtggcacgc gatgcgtttt ctcaccccga agggccgctg
    10441 ttttgctgat attcagccaa tgaccgatga atttggctgg ccgcgccgta acgcctttat
    10501 tcagccgcag gtcgatgcgg tgatgctgga aggggtgtcg cgttttccga atgtgcgctg
    10561 cttgttttcc cgcgagctgg aggccttcag tcagcaagat gacgaagtga ccttgcacct
    10621 gaaaacggca gaagggctgc gggaaatagt caaagcccag tggctggtgg cctgtgatgg
    10681 tggggcaagt tttgtccgtc gcaccctgaa tgtgccgttt gaaggtaaaa ctgcgccaaa
    10741 tcagtggatt gtggtagata tcgccaacga tccgttaagt acgccgcata tctatttgtg
    10801 ttgcgatccg gtgcgcccgt atgtttctgc cgcgctacct catgcggtac gtcgctttga
    10861 atttatggtg atgccgggag aaaccgaaga acagctgcgt gagccgcaaa atatgcgcaa
    10921 gctgttaagc aaagtgctgc ctaatccgga caatgttgaa ttgattcgcc agcgtgtcta
    10981 cacccacaac gcgcgactgg cgcaacgttt ccgtattgat cgcgtactgc tggcgggcga
    11041 tgccgcgcac atcatgccgg tatggcaggg gcagggctat aacagtggta tgcgcgacgc
    11101 ctttaacctc gcatggaaac tggcgttggt tatccagggg aaagcccgcg atgcgctgct
    11161 cgatacctat caacaagaac gtcgcgatca cgccaaagcg atgattgacc tgtccgtgac
    11221 ggcgggcaac gtgctggctc cgccgaaacg ctggcagggt acgttacgtg acggcgtttc
    11281 ctggctgctg aattatctgc cgccagtaaa acgctacttc ctcgaaatgc gcttcaagcc
    11341 gatgccgcaa tattacggcg gtgcgctggt gcgtgagggc gaagcgaagc actctccggt
    11401 cggcaagatg tttattcagc cgaaagtcac gctggaaaac ggcgacgtga cgctgctcga
    11461 taacgcgatc ggcgcgaact tcgcggtaat tggctgggga tgcaatccac tgtgggggat
    11521 gagcgacgag caaatccagc agtggcgcgc gttgagcaca cgcttcattc aggtggtgcc
    11581 ggaagtgcaa attcataccg cacaggataa ccacgacggc gtactacgcg tgggggatac
    11641 gcaaggtcgc ctgcgtagct ggttcgcgca acacaatgct tcgctggtgg tgatgcgccc
    11701 ggatcgcttt gttgccgcca ccgccattcc gcaaacactg ggcaagaccc tgaataaact
    11761 ggcgtcggtg atgacgctga cccgccctga tgccgacgtt tctgtcgaaa aggtagcctg
    11821 atatgcacgc ttatcttcat tgtctttccc actcgccgct ggtggggtat gtcgacccgg
    11881 cacaagaggt actcgatgag gtcaatggcg tgattgccag cgcccgcgag cgtattgcgg
    11941 ctttctcccc tgaactggtg gtgctgtttg cgccagatca ctacaacggc tttttctatg
    12001 acgtgatgcc accgttctgt ttaggcgttg gagcgacggc aattggtgat ttcggcagtg
    12061 cggcaggaga gctgcccgtg cctgtggagc tggcggaggc ctgtgcgcat gccgtcatga
    12121 agagcgggat cgatcttgcc gtttcttact gtatgcaggt ggaccacggg ttcgcccagc
    12181 cgctggagtt cctgctcggt gggctggata aggtgccagt tctgcctgtg ttcatcaacg
    12241 gtgtcgccac gccgctgccc ggtttccagc gtacccgcat gttgggtgaa gccattggac
    12301 gtttcaccag cactctcaat aaacgcgtgc tgttcctagg ttccggtggg ctttcccatc
    12361 agccgccggt gcccgaactg gcgaaagccg atgcccatat gcgcgaccgc ctgttgggga
    12421 gcgggaaaga tttacccgcc agtgagcgcg aattgcgtca gcaacgggtg attagcgccg
    12481 ctgagaagtt tgttgaggat cagagaactc tgcatccgct caacccgatt tgggataacc
    12541 agttcatgac tttgctggag cagggacgca tacaggaact ggatgccgtc agtaacgaag
    12601 agctttccgc cattgccgga aagtcgacac atgaaatcaa aacctgggtc gccgcttttg
    12661 ccgctatttc tgcatttggc aactggcgta gcgaagggcg ttattaccgc ccaatcccgg
    12721 agtggattgc cggatttggc tcgttaagcg ccagaacaga gaactgaata tgcaggagaa
    12781 gatgatgagt tatcagccac aaaccgaagc cgccaccagc cgttttctga atgtagaaga
    12841 agcgggtaaa acgctgcgca tccattttaa tgactgcgga caaggcgacg aaaccgttgt
    12901 cctgctgcat ggttccggcc cgggtgctac tggctgggcg aacttcagcc gcaatatcga
    12961 tccgctggta gaggcgggct atcgggtgat cctgctggat tgtccgggtt ggggcaagag
    13021 cgattcgatc gttaatagtg gttcgcgatc ggatcttaat gcacgaatcc tgaaaagcgt
    13081 ggtggatcaa ctggatatcg ccaaaatcca cctgctgggc aactcgatgg gtggccatag
    13141 ttctgtggcg ttcaccctta actggccgga gcgcgtcggc aaactggtgc tgatgggcgg
    13201 cggtacgggc ggcatgagtt tgtttacgcc gatgccaacc gaaggtatta agcgactgaa
    13261 tcagctttat cgtcagccga ctatcgaaaa cctgaagctg atgatggata tcttcgtttt
    13321 tgataccagc gatttgaccg acgccctgtt tgaagcgcgc ctgaataata tgctgtcgcg
    13381 ccgcgatcac ctggaaaact tcgttaagag cctggaagct aatccgaaac agttcccgga
    13441 ttttggccca cgtctggcgg aaatcaaagc gcaaaccctg attgtctggg ggcgcaacga
    13501 ccgctttgtg ccgatggatg cgggtctgcg tctgctgtcc ggcattgccg gttctgaact
    13561 gcatatcttc cgcgactgtg ggcattgggc gcagtgggaa catgccgacg ctttcaatca
    13621 actggtgctg aatttcctcg cacgtgctta aggaacggtc atgacgaagc atactcttga
    13681 gcaactggcg gcggatttac gccgcgccgc agagcagggc gaagcgattg cgccgctgcg
    13741 cgatctgatt ggtatcgata acgctgaagc ggcctacgcc attcagcaca taaatgtgca
    13801 atatgacgtt gcgcaggggc gtcgcgtggt ggggcgtaaa gtgggcctga cacatccgaa
    13861 agtgcagcaa caactgggcg ttgatcaacc ggatttcggg acattatttg ccgacatgtg
    13921 ttatggcgat aacgaaatca ttcctttttc ccgtgttctg caaccccgca ttgaagcgga
    13981 gatcgcactg gtgttgaacc gcgatttacc cgcaactgat atcaccttcg acgaattgta
    14041 taacgccatt gaatgggtac ttccggcgct ggaggtggtg ggtagccgca ttcgcgactg
    14101 gtcgattcag tttgtcgata ccgtggcaga taacgcttcc tgcggggtgt atgtcatcgg
    14161 cggtccggcg caacgtccgg cggggttaga cctgaaaaac tgcgccatga agatgacgcg
    14221 taataacgaa gaggtttcca gcgggcgcgg cagcgaatgc ctgggacatc cgcttaatgc
    14281 cgccgtctgg ctggcacgca aaatggccag tctgggtgaa ccgctgcgcg ccggagatat
    14341 cattcttacc ggggcattag gtccgatggt ggcagtgaat gcgggcgatc gttttgaagc
    14401 ccatattgaa ggcataggtt cagttgctgc ggcattttca agcgcagccc caaaaggaag
    14461 tctgtcatga gtaagcgtaa agtcgccatt atcggttctg gcaacattgg caccgatctg
    14521 atgattaaaa ttttgcgtca cgatcagcat ctggagatgg cggtgatggt cggcattgat
    14581 cctcagtccg acggtctggc gcgtgccaga cgtatgggcg tcgccaccac ccatgaaggg
    14641 gtgatcgggc tgatgaacat gcctgaattt gctgatatcg acattgtatt tgatgcgacc
    14701 agcgccggtg ctcatgtgaa aaacgatgcg gctttgcgcg aagcgaaacc ggatattcgc
    14761 ttaattgacc tgacgcctgc tgccatcggc ccttactgcg tgccggtggt taacctcgag
    14821 gagaacgtcg atcaactgaa cgtcaacatg gtcacctgcg gcggccaggc caccattcca
    14881 atggtggcgg cagtttcacg cgtggtgcgt gttcattacg ccgaaattat cgcttctatc
    14941 gccagtaaat ctgccggacc tggcacgcgt gccaatatcg atgaatttac ggaaaccact
    15001 tcgcgagcca ttgaagtggt gggcggcgcg gcaaaaggga aggcgattat cgtgcttaac
    15061 ccagcagagc caccgttgat gatgcgtgac acggtgtatg tattgagcga cgaagcttca
    15121 caagatgata tcgaagcctc aatcaatgaa atggctgagg cggtgcaggc ttacgtaccg
    15181 ggatatcgcc tgaaacagcg cgtgcagttt gaagttatcc cgcaggataa accggttaat
    15241 ttgccgggcg tagggcagtt ctccgggctg aaaacggcgg tctggctgga agtcgaaggc
    15301 gcagcgcatt atctgcctgc ctatgcgggc aacctcgaca ttatgacttc cagtgcgctg
    15361 gcgacagcag aaaaaatggc ccagtcactg gcgcgcaagg caggagaagc ggcatgaacg
    15421 gtaaaaaact ttatatctcg gacgtcacgt tgcgtgacgg tatgcacgcc attcgtcatc
    15481 agtattcgct ggaaaacgtt cgccagattg ccaaagcact ggacgatgcc cgcgtggatt
    15541 cgattgaagt ggcccacggc gacggtttgc aggggtccag ctttaactat ggtttcggcg
    15601 cacatagcga ccttgaatgg attgaagcag cggcggatgt ggtgaagcac gccaaaatcg
    15661 cgacgttgtt gctgccagga atcggcacta ttcacgatct gaaaaatgcc tggcaggctg
    15721 gcgcgcgggt ggttcgtgtg gctacgcact gtaccgaagc tgatgtttcc gcccagcata
    15781 ttcagtatgc ccgcgagctc ggaatggaca ccgttggttt tctgatgatg agccatatga
    15841 ccacgccgga gaatctcgca aagcaggcaa agctgatgga aggctacggt gcgacctgta
    15901 tttatgtggt ggattctggc ggtgcgatat ccgtgaccgt ttccgcgccc tgaaagcagt
    15961 gctgaaacca gaaacgcaaa ccggcatgca cgctcaccat aacctgagtc ttggcgtggc
    16021 gaactctatc gcggcggtgg aagagggctg cgaccgaatc gacgccagcc tcgcgggaat
    16081 gggagcgggc gcaggtaacg cgccgctgga agtatttatt gccgccgtgg ataaactggg
    16141 ttggcagcat ggggccgatc tctatgcgtt aatggatgct gctgacgacc tggtgcgtcc
    16201 gttgcaggat cgaccggtac gagtcgatcg cgaaacgctg gcgctgggat acgctggtgt
    16261 ttactccagc ttcctgcgtc actgtgaaac ggcggcggcg cgttatggct taagcgcggt
    16321 ggatattctc gttgagctgg gcaaacgccg gatggttggc ggccaggagg atatgatcgt
    16381 cgacgtggcg ctggatctgc gcaacaacaa ataatattga ctgccgagaa cgtgcatttt
    16441 gtatgcccat cccagcacga ctgccggatg cggcgtgaac gccttatccg gtctacattt
    16501 cgcccgtagg cagtcattaa atagttctga ttacgcggct ggcaatgtat cagtcgcgat
    16561 tcacctgcac acgcaatgag gttcatctta tgtcgactcg taccccttca tcatcttcat
    16621 cccgcctgat gctgaccatc gggctttgtt ttttggtcgc tctgatggaa gggctggatc
    16681 ttcaggcggc tggcattgcg gcgggtggca tcgcccaggc tttcgcactc gataaaatgc
    16741 aaatgggctg gatattcagc gccggaatac tcggtttact acccggcgcg ttggttggcg
    16801 gaatgctggc ggaccgttat ggtcgcaagc gcattttgat tggctcagtt gcgctgtttg
    16861 gtttgttctc gctggcaacg gcgattgcct gggatttccc ctcactggtc tttgcgcggc
    16921 tgatgaccgg tgtcgggctg ggggcggcgt tgccgaatct tatcgccctg acgtctgaag
    16981 ccgcgggtcc acgttttcgt gggacggcag tgagcctgat gtattgcggt gttcccattg
    17041 gcgcggcgct ggcggcgaca ctgggtttcg cgggggcaaa cttagcatgg caaacggtgt
    17101 tttgggtagg tggtgtggtg ccgttgattc tggtgccgct attaatgcgc tggctgccgg
    17161 agtcggcggt tttcgctggc gaaaaacagg ctgcgccacc actgcgtgcg ttatttgcgc
    17221 cagaaacggc aaccgcgacg ctgctgctgt ggctgtgtta tttcttcact ctgctggtgg
    17281 tttacatgtt gatcaactgg ctaccgctgc ttttggtgga gcaaggattc cagccatcgc
    17341 aggcggcagg ggtgatgttt gcactgcaaa tgggtgcggc gagcgggacg ttaatgttgg
    17401 gcgcattgat ggataagctg cgtccagtaa ccatgtcgct actgatttat agcggcatgt
    17461 tagcttcgct gctggcgctt ggaacggtgt cgtcatttaa cggtatgttg ctggcgggat
    17521 ttgtcgcggg gttgtttgcg acaggtgggc aaagcgtttt gtatgccctg gcaccgttgt
    17581 tttacagttc gcagatccgc gcaacaggtg tgggaacagc cgtggcggta gggcgtctgg
    17641 gggctatgag cggtccgtta ctggccggga aaatgctggc attaggcact ggcacggtcg
    17701 gcgtaatggc cgcttctgca ccgggtattc ttgttgctgg gttggcggtg tttattttga
    17761 tgagtcggag attaagaatg cagccgtgtg ccgatgcctg acgcgcctta ttaagcaggg
    17821 gcgaaaggga cttgccccca atacacccac ggcgtttaca ggtatactcg ctaaaaatta
    17881 ttcagcgggt ttggaaacaa agatggcaaa acttacctta caagagcagt tgctcaaagc
    17941 aggattagtc accagcaaaa aagcggcgaa ggtcgagaga acggcgaaaa aatcgcgcgt
    18001 tcaggcgcgt gaagctcggg cggcggtaga agaaaataaa aaggcacagc ttgagcgtga
    18061 taaacagctt agcgaacagc aaaaacaagc ggcgttggcg aaagaatata aagctcaggt
    18121 gaagcagctt attgaaatga accggatcac cattgccaat ggcgatattg gttttaactt
    18181 cactgatggc aatctgatta agaagatttt tgtcgataag ctcacacagg cgcagttgat
    18241 taatggtcgt ctggcgattg cccgcttgtt ggtcgataac aatagcgaag gtgaatacgc
    18301 cattattccc gccagcgttg ccgataaaat tgctcagcgt gatgccagca gtattgtgtt
    18361 gcacagcgcg ctcagtgccg aagagcagga tgaagacaac ccgtatgccg acttcaaagt
    18421 gcctgatgat ttgatgtggt aaacatagat aagtcgtgcg cagatgcctg atgcgacgct
    18481 atgcgcgtct tatcatgcct acggcctgcg ggcgaaatgt aggcggataa ggcgttcacg
    18541 ccgcatccgg cagtcgtgca ctattatcaa cgcatattca gtttattggc gtggtaggca
    18601 atatgctcgc caataaaact ggagacaaaa taatagctgt ggtcgtaacc ctcgtgataa
    18661 cggattaacg ttttgatatt catctcctgg cagatctttt caagattttg agtccgcagc
    18721 tgttccgcgt aaaaatcatc actcaacccc tgatcaacca tgatttccgc aacgcgttga
    18781 ccttgtgaaa taagactcac cggatggtaa tccaaccagg cctctttatt ttcaccaaga
    18841 tatgcggcaa aggcttgctg tccccacggt acttgcgatg gggagacaat gggcgaaaac
    18901 gccgagacgc tgacatattc atctgggtta cgtaacgcca gcaccagcgc gcccagtccg
    18961 cccatagaat gaccagagat agactttttg gccgttgccg gaaaatgatt catcactaaa
    19021 tccggcagct cgttgcggat atagtcatac attttgtaat gttcattcca cggcgcttgc
    19081 gtcgcgttca ggtaaaaccc ggcaccttgc ccgagatcgt aacggtcggc atctgcgaca
    19141 tgactgcctc gcggactggt gtccggcgca acaacaataa tgttgtgctc agccgcgtaa
    19201 cgctgcaccc ccgatttagt aatgaaattc tgctcattgc aggtcaggcc cgaaagccag
    19261 tacagcaccg gtaatttttc acctgcagct tttggtggga gatagacgcc gacattcatt
    19321 tcacatttca gtgatgcgga ataatgccga tacacatttt gccagccgcc aaagctggca
    19381 tgtttttcaa tgagttccat cagtcattat ctcacggtta atcccccgga cggctgcccg
    19441 gagagagtat taagaacccc gtctgaagtg gacggggtaa accagcggga aatcagtaac
    19501 gaattacggt tcgaatggat ttgccttcat gcatcaggtc gaaggcgtca ttaatttcat
    19561 ccaggctcat ggtatgcgtg acaaacggtt ccagatcgat atcacctttc atcgcatctt
    19621 caaccatacc cggtaactgg gaacgacctt tcacgccgcc aaacgcggaa cctttccata
    19681 cgcgaccagt gaccaactgg aatggacggg tggagatttc ctgaccggaa cccgcgaccc
    19741 cgatgatcac cgactgaccc cagccgcggt gcgcactttc cagcgccgca cgcatcacgt
    19801 tgacattacc gatgcattca aaggtatggt cgatacccca tttgttgata tccagcagga
    19861 catctttaat cggtttgtcg tagtcattcg ggttaatgca gtcggtggca ccgaagcgac
    19921 gcgccagatc gaatttcttc gggttggtat cgatagcgat aatccgtccc gctttcgcct
    19981 gacgcgcgcc ctgaaccact gccagaccaa tcgcgccaag accaaacaca gcaacagaat
    20041 cccctggctg gactttagct gtgttgtgta ccgcgccaat accggtggtc acgccacagc
    20101 ccagcaggca gacgtgttca tggtttgctt ctggattaat ttttgccaga gacacttccg
    20161 cgactacggt gtattcactg aatgtagaac accccatgta gtgataaagc ggctgcccat
    20221 tgtaagaaaa acgggtggtg ccgtctggca tcaggccttt accctgggtt tcgcgaaccg
    20281 caacacagag gttagttttg ccagaacgac agaactcaca ctcgccgcac tctgcggtgt
    20341 aaagcgggat cacatggtcg cctggtttga cgctggttac gccttcaccg acttcaacca
    20401 caacgccagc cccttcgtga ccgagaacca ccgggaatac accttccggg tcatcgccgg
    20461 agagggtaaa tgcgtcggta tggcaaacgc cggtatgggt gactttaatc agcacttcac
    20521 cttttttcgg tggtgcaacg tcaatttcaa cgatttccag cggtttaccg ggagcaaatg
    20581 caacggcagc acgtgatttc atctctcgct cttcctcaat atggtaatag attcagctat
    20641 ttaagatagg cacgaaccag ttcaatagtg tcgtcaacgg attggctgac ttcgcggctg
    20701 tagcagtcat ttcggtcaaa cgtttcccgg atatggcttt caagcacttc tgccatcagc
    20761 ccattagccg cgccccgaac ggcagcgatc tgttggagta tggcacggca ttcggcatca
    20821 ccctccagcg accgttccag agcatcaatc tgccctcgaa tacgacgaac tcgagtaagg
    20881 acctttttct tctcttccgg agtactgggc atttcgcacc tcatcatctg cacgcaatat
    20941 actatagggg ggtatgatat atgtcaatgc atacccccct atagtatata acaatggaga
    21001 ataattgttg tccgaagcct aaccaggtga acggtccctc aagacagatg gggaccgttt
    21061 atgtgctatt tttatgccgc atccgctaaa acaaacatcc cgtaaggatg gatttcgagg
    21121 taatagtgct caccaatatc gggctgtaaa cgggtggcgt ttacttgtaa taaaatctcc
    21181 tgtccatgcc actctacggt cacttcgtac tgcggcccca tgtaggcgac atggcgaata
    21241 acacaacgct gactctcttc gccgtgattg cttaaggtga tggcttccgg gcgaacgccg
    21301 acggtgccgc taccttgcac cgggaaatgc gccgcgcgcg gcaggcgata accgtagata
    21361 tcgacatact cttcgctaaa gttcgccggg aacaggttgg cgtcgcccat aaaacttgcc
    21421 ataaagcggg aggctggctg acgatagaga tcctgcggtg agccaatctg catgatgtga
    21481 cctttattca tcaccagcac agtgtcggaa accgcgaagg cctcactttg atcgtgagtc
    21541 acgtagagcg acgtgatatt aaactgcttt tgcagctcgc ggatcttatc gcgcatgctg
    21601 cggcgcaggt tggcatcgag gttacttaac ggctcatcaa acagcagcac ctttggtttg
    21661 aggatcaacg cgcgggccag cgccacgcgc tgttgctgac caccggaaat ctggtcgaca
    21721 tagcggtcct cgaacccttc cagatccacc attgccagcg cctctttcac ccgctgcttt
    21781 acttcgctac gcgacacgcc gagcatcttt aacccgtagc cgacgttttc gcccagcgac
    21841 atatgcggga acagagcgta tgactgaaac accatgcaga tatcgcgctg ctgaatggaa
    21901 cgatgcgtga catcttcgcc atcaataaag atttgccctt cactcggttt ttccagcccg
    21961 gcaaccagac gcaaaatggt ggttttgccg cagccggaag ggccaagcag cgtcaccatt
    22021 tgcccttgtg ggatggtgag attgatattg tcgataaccg tattactgcc gaatcgttta
    22081 gtgacgttgc gcagttccac aaaatttttc tgactcatag tgcgctccat tacgcctggt
    22141 ttttggcttt agaacgggag atacgtgctt ctccgatcag ccagtcaaag aggaaaataa
    22201 tcgccagcat caccacgatc agaatcgaac cataggcgat ggctacgcca tattcgccgt
    22261 cttcaacgcg gttaagaatg taggcggttg ccacgcgggt gtccggcgtg acgagaaaga
    22321 cgatggcgct gaccgtcgtg atggcccgca caaagctgta gatcagggcc gagagaatgg
    22381 ccggacgcag cagcggcagt aaaatgtggg ttatggttcg caaggaaccc gcgcgcaggc
    22441 tgagtgaggc ttcatccagc gatttgtcga tctgccccag gcctgcaatc ccggcgcgaa
    22501 tacccaccgg aacgttacgc atcaccatcg agataatcac aattgccgcc gttccggtga
    22561 gataaaccgg agcgctgtta aaggcaagaa tatacgaaac gcccgccacc gtacccggta
    22621 cggcaaagca cagcatggtg gtgaactcga tggtcttttt gcctttgaat tgttgacgca
    22681 ccaccaccca ggcgatgagc aggccaaaga tagcggtaat gggagcggca atcccggcgt
    22741 agagcagggt atccagcagt gaaggccacg cgccgtcgct cattccctga ccaaatagct
    22801 tgataaagtt atccagcgtc agggtgtagt ccacgcccca gttcaccgtg aagctgccgt
    22861 agaaaatgct gccgtagagc agggcgttaa aggcaatcca tatcgcaagt atggcgacca
    22921 cgctccacac cagggtgacc ggaagcggct ggacgtcgcc acggtaggat ttgccggaaa
    22981 ccgtgacgta agagcgttta ccaatccaca tatactgtat gcagaacacc agcagcgaga
    23041 acagcagcag gaaagcgcca agggtgctgg cggcctgata atccagctgc gagccggtaa
    23101 tatagaagta aatttgcgtc gccagtacgt cgaagttacc gccaagtacc agcgggttac
    23161 tgaaatcagc cagtgactgt accacgacga ttaaaaaggc attggcgagc gcgggcttca
    23221 acaacggcac aaacacgccg ttaaaggttt gccagcggct ggcgcgcagg gtgtaggagg
    23281 cctcttccag cgagggatga atggttttaa tggccccgtc gaggatcata aatgccattg
    23341 gcgtgaatgc cagaacctgt gccagccaga tcccggtaaa accgtacagc cagttggtgt
    23401 tggtcagccc aaaccactcc accataaatt ccgtaacata gccggaacgc cccatcatga
    23461 gcgtcacgcc taagccgacc acaaacggtg gcgtaacgat gggcaaaatg gaaaagatgc
    23521 gaccaataat ggcgctgcgt ttagcgatgc gcgtggtata aatcgccagc accaggccga
    23581 agaaagcaca gcctatcccc accgctatcg acaagacgat agagttgacg atcacctgaa
    23641 caatatgtgc ctgcgagagc acggtcataa acgccagcgg ggcgagctct cccgcgtcgt
    23701 tggtgaacat cgggataaaa atggcgatgc tcggccagac gataaaaatg ccaatcagcg
    23761 ccacaatcgc aatcagtgag ccgatcacga agcggtcgcc gcccagccat tcgaggcggg
    23821 ttaaggccag cgtcataatc gcgcctagtt cgacaaacag cacgatggcg gcgtagccta
    23881 aaccgcgtcc agccacggtg gcgctgatga cgataaacgc catacagagc aatgcccagc
    23941 cggcatcgaa ataatgacga ctacgctgtt cgcgcgtggc ctgacgaaac gggcggacca
    24001 gcaacaggct cgggagtaaa taccacagcc agctgatatt aaaatgcgac cagccgtacg
    24061 ccccgaggat ctcatcactc gtggattcca gcaggccata atcgaggctc cagctcggca
    24121 acaggacaaa tgccagccag cccagcaaaa cccaaaggaa aatcgcatcc cgctttttca
    24181 cgggatggag tgcaagtgtc tgtgacatag cagttccggt atggttggcc ggggcaggcg
    24241 tctgcgctgc acccggcaaa tgcgcctaat gcgggtttat ttacccatct tcacttcgct
    24301 gacccattta ttaatcaatg ccttacgaac atccgtggaa ccatatttat ccatgtcgta
    24361 gttgattaat ttcaggtcgt cgagcttcag cgaatttggc gacgtttcgg cggtggtatt
    24421 agtcaggatc tgataagact tccctttctt ccacgccagt tcctgagctt cttttgataa
    24481 tacccagtcc acaaacagtt tggcgttgtc gaggttgcgc gcgcctttca gaatgctgac
    24541 gccgccaatt tcgtagcccg taccttcaca cggggaaatc aactccagcg gcgcgccctg
    24601 ttctttttcc agcgagtagt catgcaggaa gccaatacca atcgccgttt cgccacgggc
    24661 ggcgttacgt gccggggcta tgcctgattt ggtgtactga gagacgttgg cgttcagttg
    24721 ttttagataa tcaaaggcct gatcttcccc ccacagctgc gcaaaagtag ccagggcggt
    24781 gtaggcagta ccggaacttt gcggatcggc aatttgaatt tcgcctttat attccggttt
    24841 ggtgagatct ttccagcact tcggcaccgg caggtttttc tctttcagac gatcggtgtt
    24901 tacgccaaaa ccgaggatcc ccacataaac ggcagaggac agattccctt tcacttttgc
    24961 cggatcgcgg aatttctcca tgatctgctc aaggttaggg gatttatacg cctgtagcag
    25021 gcccatctcg ccagcctgcg attgcgggtc taaagtgccg ccgtaccaga cgtcagcctg
    25081 ggggtttttc ttttcagcat cgacttttgc cagggtgctt ccggaaccgt tgcggataaa
    25141 cgaggttttt acatcatatt tctcgccaaa cgctttggtt tcggcctcac acatttcatt
    25201 ggtggcgctg caatagacca ccagccggcc ttttgcctgc gcggcacccg ttaaagtcgc
    25261 cagcgcaacg gcagaagcaa tcagagtaga ggtaaaggtc attttcattg ttttatcctt
    25321 cgagcgtgag tggtggtgat gcccgccatc agtaacggca tcagcagtaa acccataagt
    25381 actgcggcga ccgagagtaa actgaacatt cccggccagc cgtagcgttc gatgaccagc
    25441 gacagcggcc agcctgccag cgccgcgcca aggtaagcga acaggccgag aaatccggtg
    25501 attgaacccg ccgcagcttt gtgcccacac tccaccgccg caagtccaat cagcatttgt
    25561 gggccaaaga cgaaaaagcc cacggtaaaa aagcacactg ccagcagagc gtagtgatga
    25621 accggtgcca gccacagtgc ggcgactgac accatcaacc ccaacgtgaa cagcaaaatc
    25681 attggcgctc gttgcccgct aaacagcaaa tccgatcccc atccggcaaa taacgcgccg
    25741 agtaagccgc ccacttcaaa cagcattacc gtggcgttgg cgctgagcag gttgacaccg
    25801 tggctttccg tcagccagat attgccccag tcgttaagcg caatccggat cacataaacc
    25861 agtacatatg agacgcccag tagccagatc agcggatttt gcagcatggt ggtgcgcaac
    25921 atttgccaca gccccatcgg cggactttgt tgttcctgac gtaactccag cggatcgtga
    25981 cgccagtgac caactgtcgg taatccctct tcctggggcg tgcctttgag ttgtagcgtt
    26041 aaccagatcc ctaatgccat gctgattatc cccggtgtca gcatcgcggc ctgccagccc
    26101 caccagtggg cggcaaaggc gctaatcagc ggaataatcg ctccgccaat gttgatcgac
    26161 atattccagc agccccacca gaaaccgcgc tcattgcgtg agtaccagtg ggtcagcaag
    26221 cgggcacacg gtggccatcc ccagccctga aaaaaaccgt tcagtgtcca gaccacaagc
    26281 agaagcgtaa gtgattcgcc aaaggcaaac accacgttca ataacccggt ggcgaacaag
    26341 ccgacgccca taaaaccgcg ttgtccgtgg ctgtcatgcc acagtccggc ggtaaatttc
    26401 gacaatccgt agctgagata aaacagcgaa cccagcaggc caatgtcgcc tttatccagt
    26461 ccgaggtcgg tttgtagcgc cggaaggacg taattcacgc tttttcgcgt cagataaaat
    26521 gcggcgtagc caataatcat gcacatcagc aatcgcggac gtaatgtccg gtagcattga
    26581 ttaatttctc tcgctgaacg cgcgtgcatg gttgtctcca tgttgctgac tatatgaaaa
    26641 tgaaagtgaa aggggatgag agaaaatccg ggatacctaa gactttttcc tggttatccg
    26701 gcgatttgtt gcaaaaatgt gggcaagtta acaattaccc gcgtgccgtg ctgtttttcg
    26761 agcgttaagt cgccgcccag ggcgctgacg cgctcacgca tcccctgaat gccaaatcca
    26821 gccattttcc cgctggcgat ccccacaccg ttgtcgctga cttccagatg caatacctct
    26881 ccctgctggc gcaggataat cgtcacttcg ctggcttcgg cgtgtttaca gatgttattg
    26941 agcaactcct gcaataaccg atacaaggta aagcgcaccg tttcgttttc tggtgtggag
    27001 ttcagttgat aggcaaactg gcaatgaatg ccgcgttcac taaaggcaaa ttcattaatc
    27061 agatgcagca acgcttcgcg aaatgtcagt tcatcaagcg cgggaggccg caactggcgc
    27121 agcaattggc gggtggagag atgaatgcgg cgggcgagct cattgatttg gctggctgcg
    27181 gattgaattt gcgccggatc acgcgcccgc ttcaccagtt gcgactggat ctgaatggcg
    27241 gtgatgttct gaccgatttc gtcgtgcagc tctcgcgcca ggttttttcg cgtgtcctcc
    27301 tcggtgtgga tcagcttttc cgccaatgcc cgccgcgctg ccagctcggc ctccagccgt
    27361 tgtcggtaat ggtgcaggtt tagcgccaga tgttgctggc gactgatggc aatgcccagg
    27421 ccaattccca gcaaggcctg ggtcgcgagg aaaatctcca gctccaccag gttactaaag
    27481 cccacgccaa tctggcgggc gatggtgatc atcatgctgc ccaaaagccc ggagagtacg
    27541 ccaccttgcc agccaaattt ccacgccatc accacattgg gcaaaaagac cacgatcaac
    27601 agcagacgtt caatttcagg tgacagcacc atctgagtgc cgataccaat aataaaaaac
    27661 aggctacacc agatgattaa agaggtacgc aaggggggat tatgcgtatc gagtcccagg
    27721 agctggtagc gatgctgctg gcgcagaaac tcaaagataa gatagacaaa cggcgtcagt
    27781 aatacgccgc cggtaaatga ggtcaaaccc ggtagcatta ttgggcctga cataaaaggg
    27841 gagagcaggg cggtattcaa caatgaagct gccgtgacag tggctaacag caacgtcaga
    27901 cgctgccagt aaagtggaaa acggtgccag aagttttgcg caaggagggc aggaaacagg
    27961 ctaataaccg gcgttgccag catgagataa ccgttcaata gctgttcact atggagccag
    28021 aacatcatca gcaaaggggg gagcaccaac gcaggccagt agcggcgaga gagcaaaata
    28081 acaagggcga gataaactcc ctgcgggaga aacaacaccg cctgttgacc gttattggtc
    28141 agataaaagc tgagcgtcca cagcatcagc cagccggttc cccaggctag cacaataaac
    28201 agcaaaatca cccaatgacg aagattgcgc gacattaatg ccccgccagc agatggtggt
    28261 cgagcgcaaa atgtaccagt tcaatggtgc tatggcagtt cagttttccc agcacattgg
    28321 cgcgatgcac atgtaccgtt ttatgactga gttcgagttt aaaggcgatc tctttaacgc
    28381 tgtctccttt gaccagtaac tcaaacactt cacgttctcg tggggtgaga atttccagcg
    28441 cctgggcggg ttgcccgccg ccgcgtaaag cccgaatcgc atcggcacag agataatgcc
    28501 cgcccagccc gaccaagcgc accgcctgta ctaactcttc cggcccacag cgtttggtta
    28561 aatacccgct ggcaccggca tccagcgcgc tttgcacaaa ggttggcgcg tcataaatgc
    28621 taagaataat ggcgcgaaat tgcggttttt gtgcacgcag gcgcttaagc aaactgaggc
    28681 cgttttcgtc cggcatggca atatcaatga cggcaacatt gacgtcatca cggattaatg
    28741 ccgaccatgc ctgcgcggca ctactgtact gaccaatgac ctccagatcg tcttccagag
    28801 agagtaattg tgcgaagcca gagcgcacca caacatggtc atccaccagc actacccgaa
    28861 tcatactttt cttctcttat tatctaattc tcctatcatg catcacggcg cggcgctggc
    28921 aatcaaccag cgacagatat ggaatggatg taacaaaatg gcaataattt gccggagcgc
    28981 gcacatctta tctggcttgt gaaccggcgg gatacgttcg ccaaaaccgc aaaatgcgca
    29041 aactcgctaa ccattgccga tttttttcta aaaacgcttt gttcttaaca agacctttta
    29101 attcctttat caaactgacc cgcaacgaaa tactgctccc ataacaagac aggggagcag
    29161 acaatcatgg caatttcatc gcgtaacact cttcttgccg cactggcatt catcgctttt
    29221 caggcgcagg cggtgaacgt caccgtggcg tatcaaacct cagccgaacc ggcgaaagtg
    29281 gctcaggccg ataacacctt tgctaaagaa agtggagcaa ctgtggactg gcgcaagttt
    29341 gacagcggag ccagcatcgt gcgggcgctg gcttctggcg acgtgcaaat cggcaacctt
    29401 ggttccagcc cgttagcggt tgcagccagc caacaggtgc cgattgaagt cttcttgctg
    29461 gcgtcaaaac tgggtaactc cgaagcgctg gtggtaaaga aaactatcag caaaccggaa
    29521 gatctgattg gcaaacgcat cgccgtaccg tttatctcca ccacccacta cagcctgctg
    29581 gcggcgctga aacactgggg cattaaaccc gggcaagtgg agattgtgaa cctgcagccg
    29641 cccgcgatta tcgctgcctg gcagcgggga gatattgatg gtgcttatgt ctgggcaccg
    29701 gcggttaacg ccctggaaaa agacggcaag gtgttgaccg attctgaaca ggtcgggcag
    29761 tggggcgcgc caacgctgga cgtttgggtg gtgcgcaaag attttgccga gaaacatcct
    29821 gaggtcgtga aagcgttctc taaaagcgcc atcgatgctc agcaaccgta cattgctaac
    29881 ccagacgtgt ggctgaaaca gccggaaaac atcagcaaac tggcgcgttt aagcggcgtg
    29941 cctgaaggtg acgttccggg gctggtgaag gggaatacct atctgacgcc gcagcaacaa
    30001 acggcagaac tgaccggacc ggtgaacaaa gcgatcatcg acaccgcgca gtttttgaaa
    30061 gagcagggca aggtcccggc tgtagcgaat gattacagcc agtacgttac ctcgcgcttc
    30121 gtgcaataaa aggaggcgca gatgctgcaa atctctcatc tttacgccga ttatggcggc
    30181 aaaccggtac tggaagatat caacctgacg ctggaaagcg gcgagctact ggtggtgctg
    30241 gggccgtccg gctgcggtaa aaccaccctg ctgaatttga ttgccggttt tgtgccttat
    30301 cagcatggca gcattcaact ggcgggtaag cgtattgagg ggccgggagc agagcgtggc
    30361 gtagtttttc agaatgaagg gctactaccg tggcgcaatg tacaggacaa cgtggcgttc
    30421 ggcctgcaat tggcaggtat agagaaaatg cagcgactgg aaatcgcgca ccagatgctg
    30481 aaaaaagtgg ggctggaagg cgcagaaaaa cgctacatct ggcagctttc cggtggtcaa
    30541 cgtcagcggg tggggattgc ccgcgcgctg gcggcgaatc cccagctgtt attactcgac
    30601 gaaccgtttg gtgcgctgga cgccttcacc cgcgaccaga tgcaaaccct gctgctgaaa
    30661 ctctggcagg agacgggcaa gcaggtgctg ttgattaccc acgatataga agaagcggtg
    30721 tttatggcga ctgaactggt tctgctttca cccggccctg gccgcgtgct ggagcggctg
    30781 ccgctcaact ttgctcgccg ctttgttgcg ggagagtcga gccgcagcat caagtccgat
    30841 ccacaattca tcgccatgcg cgaatatgtt ttaagccgcg tatttgagca acgggaggcg
    30901 ttctcatgag tgtgctcatt aatgaaaaac tgcattcgcg gcggctgaaa tggcgctggc
    30961 cgctctcgcg tcaggtgacc ttaagcattg gcacgttagc ggttttactc actgtatggt
    31021 ggacggtggc ggcgctgcaa ctgattagcc cgctgttttt gccgccgccg caacaagtac
    31081 tggcaaaact gctcaccatt gccggaccgc agggatttat ggacgccacg ctgtggcagc
    31141 atctggcagc cagcctgacg cgcattgtgc tggcgctact cgcggcggtg ttgatcggca
    31201 ttccggtcgg gatcgccatg gggctaagcc cgacggtgcg cggcattctg gatccgataa
    31261 tcgagcttta tcgtccggtg ccgccgctgg cttatttgcc gctgatggtg atctggtttg
    31321 gtattggtga aacctcgaag atcttactga tctatttagc gatttttgca ccggtggcga
    31381 tgtcggcgct ggcgggggtg aaaagcgcgc agcaggttcg cattcgtgct gcccagtcgc
    31441 tgggtgccag ccgtgcgcag gtgctgtggt ttgtcatttt gcccggcgcg ctgccggaaa
    31501 tcctcaccgg attacgtatt ggtctgggtg tgggctggtc tacgctggtg gcggcggagc
    31561 tgattgccgc gacgcgcggt ttaggattta tggttcagtc agcgggtgaa tttctcgcga
    31621 ctgacgtggt gctggcgggg atcgcggtga ttgcgattat cgcctttctt ttagaactgg
    31681 gtctgcgcgc gttacagcgc cgcctgacgc cctggcatgg agaagtacaa tgagtgaacg
    31741 tctgagcatt accccgctgg ggccgtatat cggagcacaa atttcgggtg ccgacctgac
    31801 gcgcccgtta agcgataatc agtttgaaca actttaccat gcggtactgc gccatcaggt
    31861 ggtgtttctg cgcgatcaag ccattacgcc gcagcagcaa cgcgcgctgg cccagcgttt
    31921 tggcgaactg catattcacc ctgtttaccc tcatgccgaa ggggttgacg agatcatcgt
    31981 gctggatacc cataacgata atccgccaga taacgacaac tggcataccg atgtgacatt
    32041 tattgaaacg ccacccgcag gggcgattct ggcagcgaaa gagttacctt cgaccggcgg
    32101 cgatacgctc tggaccagcg gtattgcggc ctatgaggcg ctctctgttc ccttccgcca
    32161 gctgctgagc gggctgcgtg cggagcatga ttttcgtaaa tcgttcccgg aatacaaata
    32221 ccgcaaaacc gaagaggaac atcaacgctg gcgcgaggcg gttgcgaaaa atccgccgtt
    32281 gctacatccg gtggtgcgaa cgcatccggt gagcggtaaa caggcgctgt ttgtgaatga
    32341 aggctttact acgcgaattg ttgatgtcag cgagaaagag agcgaagcct tgttaggctt
    32401 tttattcgcc catatcacta aaccggagtt tcaggtgcgc tggcgctggc aaccgaatga
    32461 tattgcgatt tgggataacc gtgtgactca gcactatgcc aatgccgatt acctgccaca
    32521 gcggcggatt atgcatcggg cgacgattct tggggataaa ccgttttatc gggcggggta
    32581 atacgagagt ggacggtcct ctcgccccct tggggagagg gttagggtga gggggcgttc
    32641 accgtgcttt caacaggtta actccccctt tctgagagga aacaaaatta acgcagaatc
    32701 ttcttctcag ccaaatccag cgcaaagtag ctgaaaatca gatccgcacc cgcacgctta
    32761 atcgaaccta agctttcgag cacgactttc tcttcatcta tagcacccgc cagcgcggcg
    32821 aacttaatca tcgcatactc accgctcacc tgatacgcgc caatcggcaa ttcagtacgt
    32881 tcacgcagct cacgcacgat gtcgaggtac gctccagcag gtttaaccat caggcagtct
    32941 gcgccctggg cttcatccag cagtgactca cgaatcgcct cacgacggtt cattgggttc
    33001 atctgatagc ttttgcggtc gccttttaat gcgcttccgg cagcttcacg gaacggacca
    33061 taaaaggagg aggcgaactt ggtcgaatac gacataatcg ccgtatcttt aaagccagca
    33121 gcgtccagcg cctggcgaat cgcctgtacc tggccatcca tcgcggcgga aggggcgatg
    33181 aagtctgcac ctgcagcagc tgcaaccacg gcttgcttgc ctaaattttc cagagtcgcg
    33241 tcgttgtcga cgccatgctc gcacagcaca ccgcagtgac cgtgagaagt gtattcacag
    33301 aagcaggtgt ctgacatgac aatcatttct ggcaccgtct gcttgcagat gcgcgacata
    33361 cgcgctacca gtccatcttc ccgccaggca tcgctgccgg tttcatcggt atggtgagag
    33421 atgccgaaag tcatcacgga acgaataccg gcgttggcga tgcgttcaat ttcgcgtgcc
    33481 agatgtttct ctggaatgcg catcacgcca ggcatggctt caacggcttt gtagtcgtca
    33541 atttcttctt caacaaagat cggcaacacc aggtcgttaa ggctaagtgt tgtctcttca
    33601 aacatagcgc gcagcgcagg agatttgcgc aggcgacgag ggcgttggat taagtctgtc
    33661 atggtctgcc tgatgtttgt ggaattaagg tgcacagtat acctgaagcg gggctggagt
    33721 gttttacgaa agttgtcacg atgttgacaa gaagagaatg aaagagagac cagggcatct
    33781 ttgtaata
//
LOCUS       DAZKIF010000042        33444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZKIF010000042 DAZKIF010000000
VERSION     DAZKIF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.042"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(62..1243)
                     /locus_tag="ACHOTW_001980"
     CDS             complement(62..1243)
                     /locus_tag="ACHOTW_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9844093.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
     gene            complement(1439..1533)
                     /locus_tag="ACHOTW_001981"
     tRNA            complement(1439..1533)
                     /locus_tag="ACHOTW_001981"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1497..1499),aa:Sec,seq:tca)
     gene            1826..3208
                     /locus_tag="ACHOTW_001982"
     CDS             1826..3208
                     /locus_tag="ACHOTW_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP9844094.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3218..5536
                     /gene="yicI"
                     /locus_tag="ACHOTW_001983"
     CDS             3218..5536
                     /gene="yicI"
                     /locus_tag="ACHOTW_001983"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP9844095.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5589..7298)
                     /locus_tag="ACHOTW_001984"
     CDS             complement(5589..7298)
                     /locus_tag="ACHOTW_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9844096.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(7419..8810)
                     /gene="xanP"
                     /locus_tag="ACHOTW_001985"
     CDS             complement(7419..8810)
                     /gene="xanP"
                     /locus_tag="ACHOTW_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP9844097.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9090..10295
                     /gene="gltS"
                     /locus_tag="ACHOTW_001986"
     CDS             9090..10295
                     /gene="gltS"
                     /locus_tag="ACHOTW_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP9844098.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10298..11164
                     /locus_tag="ACHOTW_001987"
     CDS             10298..11164
                     /locus_tag="ACHOTW_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844099.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11149..13230)
                     /gene="recG"
                     /locus_tag="ACHOTW_001988"
     CDS             complement(11149..13230)
                     /gene="recG"
                     /locus_tag="ACHOTW_001988"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9844100.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13236..13925)
                     /gene="trmH"
                     /locus_tag="ACHOTW_001989"
     CDS             complement(13236..13925)
                     /gene="trmH"
                     /locus_tag="ACHOTW_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP9844101.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(13932..16040)
                     /gene="spoT"
                     /locus_tag="ACHOTW_001990"
     CDS             complement(13932..16040)
                     /gene="spoT"
                     /locus_tag="ACHOTW_001990"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9844102.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16059..16334)
                     /gene="rpoZ"
                     /locus_tag="ACHOTW_001991"
     CDS             complement(16059..16334)
                     /gene="rpoZ"
                     /locus_tag="ACHOTW_001991"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9844103.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16389..17012)
                     /gene="gmk"
                     /locus_tag="ACHOTW_001992"
     CDS             complement(16389..17012)
                     /gene="gmk"
                     /locus_tag="ACHOTW_001992"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9844104.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17270..18952
                     /gene="ligB"
                     /locus_tag="ACHOTW_001993"
     CDS             17270..18952
                     /gene="ligB"
                     /locus_tag="ACHOTW_001993"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP9844105.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(18949..19566)
                     /locus_tag="ACHOTW_001994"
     CDS             complement(18949..19566)
                     /locus_tag="ACHOTW_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP9844106.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19858..20682)
                     /gene="dinD"
                     /locus_tag="ACHOTW_001995"
     CDS             complement(19858..20682)
                     /gene="dinD"
                     /locus_tag="ACHOTW_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP9844107.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(20903..21766)
                     /locus_tag="ACHOTW_001996"
     CDS             complement(20903..21766)
                     /locus_tag="ACHOTW_001996"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9844108.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21893..22609
                     /gene="rph"
                     /locus_tag="ACHOTW_001997"
     CDS             21893..22609
                     /gene="rph"
                     /locus_tag="ACHOTW_001997"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9844109.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22675..23316
                     /gene="pyrE"
                     /locus_tag="ACHOTW_001998"
     CDS             22675..23316
                     /gene="pyrE"
                     /locus_tag="ACHOTW_001998"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9844110.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(23353..23949)
                     /gene="slmA"
                     /locus_tag="ACHOTW_001999"
     CDS             complement(23353..23949)
                     /gene="slmA"
                     /locus_tag="ACHOTW_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP9844111.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(24056..24514)
                     /gene="dut"
                     /locus_tag="ACHOTW_002000"
     CDS             complement(24056..24514)
                     /gene="dut"
                     /locus_tag="ACHOTW_002000"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9844112.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24492..25712)
                     /gene="coaBC"
                     /locus_tag="ACHOTW_002001"
     CDS             complement(24492..25712)
                     /gene="coaBC"
                     /locus_tag="ACHOTW_002001"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP9844113.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25884..26552
                     /gene="radC"
                     /locus_tag="ACHOTW_002002"
     CDS             25884..26552
                     /gene="radC"
                     /locus_tag="ACHOTW_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9844114.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26769..27005
                     /gene="rpmB"
                     /locus_tag="ACHOTW_002003"
     CDS             26769..27005
                     /gene="rpmB"
                     /locus_tag="ACHOTW_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9844115.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            27026..27193
                     /gene="rpmG"
                     /locus_tag="ACHOTW_002004"
     CDS             27026..27193
                     /gene="rpmG"
                     /locus_tag="ACHOTW_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9844116.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            27291..28100
                     /gene="mutM"
                     /locus_tag="ACHOTW_002005"
     CDS             27291..28100
                     /gene="mutM"
                     /locus_tag="ACHOTW_002005"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP9844117.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(28139..28618)
                     /gene="coaD"
                     /locus_tag="ACHOTW_002006"
     CDS             complement(28139..28618)
                     /gene="coaD"
                     /locus_tag="ACHOTW_002006"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9844118.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28626..29903)
                     /gene="waaA"
                     /locus_tag="ACHOTW_002007"
     CDS             complement(28626..29903)
                     /gene="waaA"
                     /locus_tag="ACHOTW_002007"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP9844119.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            30316..31374
                     /gene="rfaQ"
                     /locus_tag="ACHOTW_002008"
     CDS             30316..31374
                     /gene="rfaQ"
                     /locus_tag="ACHOTW_002008"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP9844120.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31371..32495
                     /locus_tag="ACHOTW_002009"
     CDS             31371..32495
                     /locus_tag="ACHOTW_002009"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9844121.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            32488..33285
                     /gene="rfaP"
                     /locus_tag="ACHOTW_002010"
     CDS             32488..33285
                     /gene="rfaP"
                     /locus_tag="ACHOTW_002010"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP9844122.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7906 a         8302 c         8900 g         8336 t
ORIGIN      
        1 ccatccgcct gttggggctg gatggttatt cattattttt gtgtcaccga actttcattc
       61 atcaggccag ctcagaaagt gaatcaatgt agtcagcgta ccattgcatc atttcacggc
      121 gaccatcgag atactgggcg tggttatagg tacccctgat ggagttttta tccacatgag
      181 ccaactgcat ttcaatccag gcagaattaa agccttgttc gtgcagaatt gtgctcatgg
      241 tatgcctgaa accgtgccct gtgagtcttc ccttataccc cagcaattca atgacctggt
      301 taatgctctc cttactaatt ggcttgcgag gatcgttcct accgataaag accagaggat
      361 gatgttttga gattggttca agttgcttaa atagcgcaat aacctgctca gagggcggca
      421 caatatgagg ccgtttcatc ttcattactt ctgccgggat ctcccataat tttgcctcaa
      481 aatcaatatc ctcccaatgc gcgaaacgca actcttgcgt tcgtacaccg gttaacataa
      541 tgatctgagt agctgtctta gtgatgatac ttccggtata acccgccaga tcagtaagga
      601 agtggggaag ttcattggca gtaagaaagg gaaaatgtac ttttttcggt gtagctaaag
      661 cactggcaag atcgggagca gggttatagt cggctcgacc agtaacaatt gcatagcgaa
      721 acacctcacc acaacgttgg cggaccttgc gcattttctc cagtgctcca cgtttctcca
      781 tcttacgtag tgcttccaat agctccatcg gcttgatttc ggcaataggt cgcttgccga
      841 tgtaggggaa gatatctttc tcaaaggtgt cgattatttc atcgcggtag cgcagtgacc
      901 agcgatcggc tttcgtctga tgccactcgc gagcaacggc ctcgaaagtg tttttcaaac
      961 tcatttgcag cgctattttt tcttcttttt ttgcctcacc cggatcgcca ccagccgcaa
     1021 gtattttcct ggcttcactg cgtttctcac gtgcgtcggc aagcgttaca tcggggtata
     1081 cgccaataga cagcatcttt tctttaccgg cgaagcggta cttcatacgc cagtatttgg
     1141 aaccattagc gttaatcagc agatacatac ctccgccatc agccagctta tagggccttt
     1201 cttttggctt cgcggttttc actttaacat cggttagcgc catgacatcc tccagctttt
     1261 gatgaatcca tcctcctggg ggaatttccg ccagtaacca acctgatacc cccaatgcac
     1321 ccccacaaac aacttgatat gggttaaagt cgtttgacct caggatacaa agaaaacctg
     1381 atatataagg gattataagg ggctttgttg atgtcgggag agttcaggag aggcaaactg
     1441 gcggaagatc acaggagtcg aacctgcccg ggaccgctgg cggccccaac tggatttgaa
     1501 gtccagccgc ctcaccggag acgacgatct tccgcgcctc gattgctaca tggaggcggg
     1561 gcgcattata gctacttcct tgagtttcta catcccccag atcagttttt tccgcctaat
     1621 acacgcagcg tattatcacg aaagcattat aattcatata aaactcatat tttacaatga
     1681 tatatgagcg gaattaacgc taccacgatc acataaaaca agaatcataa attaataacc
     1741 agatatcgga atattcgctc tcacagggat ggtttacaaa atgcgttatc ggtctcagca
     1801 cccctatagc atcaaggaaa agcagatgaa gagtgaagtg ttgtccgtta aagagaaaat
     1861 tggttatggc atgggagacg ccgccagcca cattattttc gataacgtaa tgttatatat
     1921 gatgttcttt tataccgata attttggcat acctgccgga tttgtcggaa ccatgttttt
     1981 ggtcgctcgt gcactggatg cgatttccga tccttgcatg gggttgttgg ccgatcgaac
     2041 gcgctctcgc tggggtaaat ttcgtccgtg ggtactgtgt ggcgcactgc catttgggat
     2101 cgtctgtgta ctggcctata gcacgccaga tctcagtatg aacggcaaaa tgatctatgc
     2161 agcaattact tacaccctac ttaccttact ttataccgtc gtcaatatcc cttactgcgc
     2221 attgggtggt gtaatcacca atgacccgac tcagcgtatc tcgctgcaat cctggcgttt
     2281 tgtgctggcg accgcgggag gcatgctctc tactgttctg atgatgccac tggttaattt
     2341 aattggcggt gataataaac cactcggttt ccagggcggt atcgcggtcc tttccgtggt
     2401 ggcattcatg atgctggcat tctgtttctt caccactaaa gaacgcgttg aagcaccacc
     2461 tacaacgacg tctatgcggg aagatttacg tgatatctgg caaaacgacc agtggcggat
     2521 tgtcggttta ctaaccattt tcaatatcct ggcggtgtgc gtccgcggtg gagcgatgat
     2581 gtattacgtc acatggattt tgggtacgcc ggaagtgttt gtcgcttttc tcaccactta
     2641 ttgcgtgggt aacctgattg gttccgcact ggcaaaaccg ctgaccgact ggaaatgtaa
     2701 agtcactatc ttctggtgga cgaacgccct gctggcagtg attagcctcg cgatgttctt
     2761 tgttcctatg caggccagca tcactatgtt tgtcttcatc ttcgtgattg gtgtattgca
     2821 tcaactggtg acacctatcc agtgggtaat gatgtccgat accgtcgact acggcgagtg
     2881 gtgcaatggt aaacgcctga ccgggatcag ttttgctggc acgctgtttg tgctcaaact
     2941 ggggttggcc ttcggcggcg ctcttatcgg ctggatgctg gcttatggcg gatatgatgc
     3001 ggcagaaaaa gcgcagaaca gcgccacgat tagcatcatt attgcgctat tcacgattgt
     3061 tccggcgatc tgttatttgc tgagcgcgat tatcgctaaa cgctactact cactcacgac
     3121 gcacaatctg aaaaccgtta tggaacagct ggcccagggc aaacgccgtt gccagcaaca
     3181 attcacctct caagaagtgc agaactaagg aacggcaatg aaaattagcg acggaaactg
     3241 gttgattcaa cctggcctca atttgattca cccgcttcag gtgttcgagg ttgaacagca
     3301 gggtaatgaa atggtggtct atgctgcccc ccgtgatgtg cgtgaacgaa cctggcagct
     3361 tgatacgcct ttatttacgc tgcgcttttt ctccccacag gaaggtattg tcggtgtacg
     3421 gattgagcat tttcaggggg cgctgaataa cggtcctcat tatccgctca atattttgca
     3481 agacgtgaag gtcacaatcg aaaacacaga acgttacgct gagtttaaaa gtggcaactt
     3541 aagcgcgcgt gtcagcaaag gtgagttctg gtcactggat tttctgcgca acggcgaacg
     3601 tattaccggt agtcaggtga aaaataatgg ctacgtgcag gacacgaata atcaacgaaa
     3661 ttatatgttt gagcggctgg atcttggcgt tggcgaaaca gtttacggtc tgggagagcg
     3721 ctttactgcc ctggtgcgca atggccagac ggtagagacc tggaaccggg acggcggcac
     3781 aagtactgaa caggcgtata aaaatatccc gttctatatg actaaccgtg gctatggggt
     3841 actggtcaat catcctcaat gcgtctcttt tgaagtggga tcggagaaag tctccaaagt
     3901 gcagttcagc gttgagagtg aatatctcga atactttgtt attgacggcc cgacgccgaa
     3961 agcggtactt gatcgttata cccgttttac tggtcgtccg gcgctgccgc ccgcgtggtc
     4021 cttcggcctg tggctaacca cttcatttac caccaactac gacgaagcga cggtaaacag
     4081 ctttatcgat ggtatggcgg gacgcaatct gccactgcat gtttttcact ttgactgttt
     4141 ctggatgaaa gccttccagt ggtgcgattt tgagtgggac ccgctgactt tcccggaccc
     4201 ggaagggatg atccgccgcc tgaaagcgaa agggctaaaa atctgcgtct ggattaaccc
     4261 ctatatcggg caaaaatccc ctgtctttaa agagttacaa gagaaaggct atttactcaa
     4321 acgcccggac ggttcgttat ggcagtggga taaatggcag ccaggtctgg cgatttatga
     4381 ctttaccaat ccggatgcct gcaaatggta cgccgacaaa ctgaaaggtc tggtcgcgat
     4441 gggcgttgat tgctttaaga ccgactttgg cgaacgtatt ccaatcgatg ttcagtggtt
     4501 tgacggttcc gatccgcaga aaatgcataa ccattatgcg tacatctaca acgaactggt
     4561 gtggaacgtg ctcaaggaca ccgttggcga ggaagaagcc gtcttgtttg ctcgctcggc
     4621 ctccgttggt gcgcagaaat tcccggtaca ctggggtggt gactgttacg ctaactacga
     4681 atcaatggcg gaaagcctgc gcggtggttt gtctattggc ctttcaggtt ttggtttctg
     4741 gagccacgat atcggcggct ttgagaatac cgctctggcg cacgtttaca aacgctggtg
     4801 cgcgtttggt ttgctctcca gccatagccg tttacatggc agcaaatctt atcgtgtgcc
     4861 gtgggcctac gatgatgagt cctgtgatgt ggtgcgcttc ttcacgcaac tgaaatgccg
     4921 catgatgccg tatctgtatc gtgaagctgc tcgtgcgaac gcgcggggta cgccgatgat
     4981 gcgggccatg atgatggagt tcccggacga tccggcttgt gattaccttg accgtcaata
     5041 catgttaggc gacaacgtga tggttgctcc ggtgttcagt gaagcgggcg atgtgcagtt
     5101 ctacttgccg gaaggtcgct ggacacatct gtggcacaac gatgaactcg atggtagtcg
     5161 ctggcataaa cagcagcaca gcttcctgag tctgcccgtt tatgtgcgtg ataacaccct
     5221 actggcgctg ggcaacaacg agcaacgtcc cgattacgcg tggcacgaag gcacggcatt
     5281 ccagctcttt aatctacaag acgggcatga agccatctgt gaagtgcccg ctgctgacgg
     5341 ttccgttctt ttcaccctga aagcggcgcg tactggcaac acgattactg tgactggtgc
     5401 gggcgaggcg aagaactgga cactgtgctt gcgcaatatt gtgaaagtaa atggtctgca
     5461 aggtggttcg caggctgaaa gtgagcaggg gctggtggtg acgcctcaag ggaatgcgct
     5521 gacaattacg ttgtaatcta ttgatggaat gccgggtacg tctgagtcgt acccggcgag
     5581 attgattttt actgcaccgg ctctgtcgat gtaacttcgc tactggaaac gacgcccgca
     5641 ttcatggttt gagtcacttg ctgctttgcg gcgttcacgg catggagttg cccgctaacc
     5701 gtaggtttca gcgggacatt ggcctgaata tcgccactgg cggtaagctg aatattgccg
     5761 tcgccagtca acggtaattc aggccatccc cattgttgca aaatattcac cggcacaccg
     5821 cgtccattca ggctgatatg ggtctgacgt tgtggcgttt gtgaaacact ggcagtggct
     5881 tccagaatgc ctttttcagt aaatgcactc agttcgctga tattcaccgt gctgctgttg
     5941 gcggtcagcg ccagcgacgg gcgacgaaca tcaacacgat tgaatgtcgc ggcggcggca
     6001 ttcagattcg ccgagccact ccagacgccc catttatgat cggtaaccag cgtcaggttg
     6061 gcaccgtaac catcgagcgt ggtgagctgc cacgggaagt cagggtcgat atcaatgatc
     6121 agattgcggc tggcgctaaa tctcttcagt tgcaggctgt ttaaccaacc gggtgtcgtt
     6181 tccatccaca actgttgcca gtttttcggc aaggtatatt ccagcccggc aattgccgca
     6241 tcatcaagga tcaacgtttt cccgtcacgc agccagttcc ctgacgttct gaccataccc
     6301 ccttcccagc ggctggtgaa ctggcgcagc gctacgccct gcggggaaaa ttccgcgttt
     6361 ataatcgggt caaataaatg cagcgaacca taaatgaact cgctagcgtt catcgacagt
     6421 ttgccttctt gtgtctgcca gtcatcttta ctgaaggtca tgttgcgcaa gctgagatcg
     6481 aggtcggtca ccgcccagtc cggaccttgc aaacgagcat cgatcacttc caggcgacca
     6541 atctgcaacg acgggacaga gcgtaatggc gcaaagaagt cggttagcga tttttcgctt
     6601 tgtagacgga tatccgccat gcgcagattt tccacttgcc agctgccgtc ggcgttacgc
     6661 tgcgcgtttc cggttaatgt cccgcgggcg atgtcggcac ccaggttagt cagcgtaacg
     6721 cgatcgttat caatactgcc ttcaatcagt acattggtgg caggaacatc attgagcgaa
     6781 agcgatccgg cactaaactg aatctgcgcc ttcgtaccca gcactttacc ggcttctggt
     6841 gaccacggaa ccacgccgcc atttacccgc tgcgcgctca gtttccattc gctattcggg
     6901 ctattaaacg ccatatcacg cagttgcaga cgatcggctt tgaacggtag cggcgcggtc
     6961 tggtcggtga gattcagcgt cccgttttcc agcaggatgg tatcgacatg gcgtggttcg
     7021 gtcagttgcc gactgcttag cgcaatgtcg acactttttg ccaccagggt cgcgggctga
     7081 ccatcacgac caaacgtgac gttctccagc acgatatgag atggcgcgga aaaacggtga
     7141 tccatcgccc cgaaggccag atgatagtcg ctattctcgg aaacccatgc gctgatatgt
     7201 tctgctcccc agcgggtttg cagaagaaaa tagaggccag cgatcaccac taacagagcg
     7261 atgagaatgt agagaagcag cttcccaata aatttcatgg tcttccatcc cgtgaaatgc
     7321 acataaggga gttatgcacg atttacgcgc aatcctcaag gcgggaatgg tgaaagaggt
     7381 gagactgcgg cggtaattat cttaccgccg ccagtgaatt actgtttttc tggtgggaaa
     7441 atcagattca gcacgatggc agtaataccg cccgcggcga tcccggagga gagcaggttt
     7501 ttcagccatt caggggcaaa ctgcaaaatc agcggctgct gagacacgcc cagaccaacc
     7561 gccagcgaca gcgcgataat cagaatcgcc cgacggttca gcggctcacg agaaacgata
     7621 cgcacaccgg aggcggcgat ggtgccaaac attacaagcg ttgcgccgcc cagaaccggt
     7681 tctggaatgt gttgtacaaa accgctcact gccgggaaca gacccagcac gatcaacatc
     7741 agcgcgacga caaaaccgac atagcggctg gcaacaccag tcaactggat cactccgttg
     7801 ttttgcccga agcaggagtt cgggaaggtg ttaaacaccg ccgaaacaaa cgagttcagg
     7861 ccgtttgcca gcacgccgcc tttcaggcgt ttcatgtaca gcggaccgga cactggctgt
     7921 tcggaaacgt cagaggtcgc cgtgatatcg ccaatggttt ccagcgaagt gatcataaag
     7981 accagcatca gcggcagcag cagactccat tcaatgccaa gaccgtaata gagcggcgtt
     8041 ggcaccataa tcagttcttg cgtcatcggt tcgttgcttt ctggcaacat gcccataaac
     8101 cacgccagcg catatccggc cgccatcgca atcaccagtg aggccacgcg taagtaaggg
     8161 ttacgttgac ggttaagcag gataattaag gctaagacca cgcctgccag cagcagattt
     8221 ttcggtgcgc cgaaggtgtt atcgctcatg gctgcgtaac cgccgccaat ggacgttaac
     8281 ccaacctgaa ttagcgacag gccgataatc atcaccacaa cgccagaaac cagcggcgta
     8341 ataatgcggc gcgccagatg cagaacgcgg gagatcacca tctcggtgca acttgccagc
     8401 atcaacgtgc cgaacaaagc cgccatcatg gtaggaacat cagcaccacc ggttttcagc
     8461 gcggtaccgc ccataatcag cggggcaaca aagttgaagc tggtgccctg aatagacaac
     8521 agcccggagc caaccggacc ccaggcctta atttgaataa tcgatgccac accggaggca
     8581 aacagcgaca tactaataat gtgttgcgtg tcttgtgccg gtaaacccag cgcctggcag
     8641 attaatagcg ctggcgtgat caccgcaacg aacatcgcca gcagatgctg acaggcggca
     8701 aacagggttt gaggaagcgg cggacgatct tcaagacggt aaatcagttc gctgttttga
     8761 gtctgcgcaa ccggttgcgc attttctgac tcgagggtgg aaacagacat cgaaaacaat
     8821 cccgtggtag caaagcgggc attttagctg accgaaagag aaaagcaaac gtttgccaca
     8881 tcctcatcta aatatttaaa tgagataaat gcactttttt atataacttt tggtattttt
     8941 ctgcttaaaa tccatgccat ttgttcatta acagctgaga atttgaccca ggaaagcata
     9001 actcgatacc cgtcatagtt catgaagttg ccactgcgcg atgaagtatg acgagtatga
     9061 aagagtgatg cggatacaaa ggagtaacta tgtttcatct cgatacttta gcaacgcttg
     9121 ttgccgcaac gctgacgttg ttgctcgggc gtaagttggt ccattccgtc tcctttttga
     9181 agaaatacac cataccggaa cctgttgcgg gtggtttgtt ggtggcgctg gcgctactag
     9241 tactgaaaaa aagcatgggc tgggaagtca actttgatat gtccctgcgc gatccgttaa
     9301 tgctggcttt cttcgccacc attggcctga acgccaacat tgccagtttg cgtgccggtg
     9361 ggcgtgtggt tggcatcttc ttgattgtgg ttgttggtct gttggtgatg caaaatgcca
     9421 ttggcattgg tatggctagc ctgttagggc ttgatccgct gatggggctg ttggccggtt
     9481 ctattacgct ttccggcggt cacggtacgg gcgctgcgtg gagtaaattg ttcattgaac
     9541 gttatggctt caccaatgcg acggaagtgg cgatggcctg tgcaacgttc ggtctggtgc
     9601 tgggcggctt gattggcggt ccggtggcgc gctatctggt gaaacactcc accacgccga
     9661 acggtattcc ggatgaccag gaagtcccga cggcgtttga aaagccggat gtgggccgca
     9721 tgatcacctc gctggtgctg attgaaacta tcgcgctgat tgctatctgc ctgacggtgg
     9781 ggaaaattgt tgcgcaactt ttggctggca ctgcttttga actgccgacc ttcgtctgtg
     9841 tactgtttgt tggcgtgatt ctgagcaacg gtctgtcaat gatgggcttt taccgcgtct
     9901 ttgagcgtgc ggtatccgtg ctgggtaacg taagcctgtc gttgttcctg gcgatggcgc
     9961 tgatggggct gaaactgtgg gagctggctt cgctggcgct gccgatgctg gcgattctgg
    10021 tggtacagac catcttcatg gcgttgtatg ccatcttcgt tacctggcgc atgatgggca
    10081 aaaactacga tgcggcagtg ctggctgcgg gtcactgtgg ttttggcctc ggtgcaacgc
    10141 caacggcaat cgccaacatg caggcgatca ctgaacgctt tggcccgtcg cacatggcgt
    10201 ttttggtggt gccgatggtc ggtgcgttct ttatcgatat cgtcaatgcg ctggtgatta
    10261 agctgtattt aatgttgccg atttttgccg gttaaaaatg aaaaagcgtc ggaaggaacc
    10321 ggaaacgcta cgtgaacact gtcgccatat ttttggtgat gagccgccag tgctctgtgt
    10381 atgggaaacg gaatttgatt acgcagatgc cgaactcaag gcgctggcgg ctaaagagtg
    10441 gcagcagatc agcgaatggg atttgagtgc ttactatgta ctcaatctcg tttataacga
    10501 accgatgcaa atagagttgt ttcgctatct gtttccgctc tgcctggcgc aatggcatga
    10561 aaccgtttta gctggcggtt atggtgatca cttcgaagaa tccttaatga aggcattgtg
    10621 tcggccctat ctctggcagg agatgatgaa tgcttcccaa cgccagcagg tacggcaatt
    10681 tttactggac accgcgttgc agcgtatgga taacgagcgt ggtttcaata acgttctttg
    10741 ctggctggcg gtgttcaata ctctgggtgg ggccgcgccg ttaattcgtt ctctctggtc
    10801 gcgctggtgg gcgctggata cgccaggaaa agcggtgtgt gctattcagt atgccgcgca
    10861 tctaatttat cccatagaag ccaatccgtt atggtcgcaa gaatggatag gctgggggca
    10921 tccattaggc cataaagatg gctggtcctc tgataatcgc gcttttcttc gccaaatgtt
    10981 aacacctgaa atgattgtgg ctggcgtaca ggcagcggct gaaattctgc gtggtgaacc
    11041 ggaaggcgca atggcggcgc gaattgcaca ggatgcgtat gaggcaatgg atatcctcac
    11101 tatccagatt gaagatttac tgcgtgactt gtcttgtgat gagtctggtc atgcgttgga
    11161 gtaacgttcc gtctccggca tccagcgttc tatcagggct tttgcttgtt gtgggtaacg
    11221 ttcgtgaata tgtcgcgcca ggcgctgaac ttccgggatc atcgcctgat cgcgcagtaa
    11281 atccgccact ttaaactcag cattacccgt ctgacgcgta cctaacagtt cacccggtcc
    11341 gcgaatctct aagtctttct gtgcaatcac aaaaccgtcg ttgctgtcgc gcagcacttg
    11401 cagccgaatt tgcgcggttt tggagagcgg cgttttgtag agcagcacgc agtgagaagc
    11461 caccgcacca cgacctacac gcccgcgaag ctggtgtaac tgcgccagac ccagacgctc
    11521 agggttttcg ataatcatca ggctggcgtt aggcacatca acaccgactt caataacggt
    11581 agtggcaact agcagatgta gctcaccttg tttaaacgac gccatcaccg cctgtttctc
    11641 ggctggtttc atccgcccgt gtaccaggcc aacattcaat tctggtagcg ccagtttcaa
    11701 ctcttcccag gtagcttccg ccgcctgcgc ttccagtaac tctgactctt caatcaacgt
    11761 acaaacccag tacgcctgac gaccttcagt tatgcaggcg tggcgcacgc ggtcaatgat
    11821 gtcggtacgg cgggtatcag gaatagcgac cgtagtcact ggcgtgcggc ctggcggcag
    11881 ctcatctatc accgaggtat cgagatcggc atacgccgtc attgccagcg tgcgggggat
    11941 cggcgtggcg gtcatgatca actgatgcgg atggaagcct tgctgctggc ctttctccca
    12001 taacgccaga cgctgatgca cgccaaagcg atgttgttcg tcgataatca ccagcgccag
    12061 accgttaaac tgcacctgtt cctggaagat ggcgtgcgta ccaacaatca tctgcacctg
    12121 accgctggcg atggcttcct gctgagacag tcgcgctttg cctttctgct taccggcgag
    12181 ccagcccact tcgataccga gcggttcaaa ccagttgcgg aagttattgg cgtgctgttc
    12241 ggcgagtaat tcggttggtg ccatcaatgc cacctgtttg ccgtgggcaa tcgcacgcag
    12301 cgcggcgagg gcggcgacca gcgttttacc ggaacctaca tcgccctgta ccaggcgcat
    12361 catcggcaca tccagcgcca tatcgcgctc gatctccgcc actacgcgtg cctgtgcgcc
    12421 cgttggcttg aacggtaagg ctgcgaggag tttatttttc agcgcatcat tggcgctcag
    12481 cggctgggca tgaaaacgct gtgctccggc acgtaaggct aacatgctga ggttgtgcgc
    12541 cagcagttct tccagaatca gacgacgttg cgctggatgc tgcccggttt ccagatcgct
    12601 aagctgtagc gtcggtggcg ggcggtgcaa agtgcgcaac gcttccggta gcgtcatcat
    12661 tccttgtgac agttccggcg gcaggagttc ttcaatggcg caggtgtcga gcagatccag
    12721 cgcctggtcg gttaatttac gcagcgtggc ctgctttacg ccttccgttg ttggataaac
    12781 cggcgtgagc gtttcctgta attctggcgt gctgagatcg ccctgcacgc ggtattccgg
    12841 gtggatcatc tccgcaccat atttaccgcg ctttgcttcg ccataagcca gtacacggcg
    12901 gcccgtcgcc aggctatttt tcattgccgc gctgaaattg aaaaagcgca tggtgaggat
    12961 gccggaaccg tcgctgatct ggcaggtcat catccgccga ccgccgaagg agatattgca
    13021 gttcagcact tcgccttcca ccgtggcata aacgcccggc agtagttctc cgatggggta
    13081 gagatgggtg cgatcttcgt agcgcagggg aaggtgtaag agtagatcct gcacggtatg
    13141 caggttgatt ttcgccagtt tgttactaag tgctgcgcca acgcccgtta gggaactgag
    13201 tgggacagca tctaacaggc gacctgtcat ggcacttacc ctgcagcctg catagtagcc
    13261 caccagtcgg catcagcttc gatctcgcct tgctgattga cgtggggata aggcaggcct
    13321 ttgcgttttg cgactttcgc cagcaccgga tagccgcctt caaacaacag gcgttgttgc
    13381 tctgcttccg gcaacatgct gttttcacgc aggtacatgc ctgcattttg ccgctgacgc
    13441 tgggcttcgt aaagaatgag ggctgaggca acggaaacat tcagcgactg caccatgccg
    13501 atcatcggaa tgatgatgtc ctgatccgcc agggccaatg cttcctgcgt gatgcccgtt
    13561 ttctcctgtc ccatcaaaat gcaggtcggg cgagtgtaat caatttcgcg gaaatcgaca
    13621 gcgttatcag aaagatgggt tgccagaatc tgcatgccct ggcctttgag atgagcgacg
    13681 gcatcgccaa tggtgcggtg tgttttcacc tgtacccagc tgttactacc cgccgctgcc
    13741 gaagccatgg tgcgcatgcg gctaccaggc cagacggcgt gaacttcatg tacgccaacg
    13801 gcatctgcgg tacgaataat cgcagaaacg ttatgaggtt tgtggacctg ctccatgcag
    13861 acggtcagat caggctgccg cctggcgagc atttcgcaga tgcgtgcata acgtgttggg
    13921 ttcataaaac attaatttcg gtttcgggtg actttaatca cgtctggcat cacgcggatt
    13981 ttgcgcatga tattcgccag atgcacacgg tcacgagcgg tcagacgaat aaaggcgctg
    14041 tagacgcgac catctttctc ttccgtattc aaactttgaa tattcgaggt cgtggtgtta
    14101 attgccgccg tcaggtttgc cagcgcaccc tgatgattga acatctccac cttgatttcg
    14161 gtgatgaact cctgcgccgt ctctttatcc cattccacag ccataaactt ctctggctct
    14221 ttctggtagc cacggatgtt acggcaggat tcatggtgga tcaccagacc tttaccgggg
    14281 ctgacgtggg cgataatcgg gtcgccagga atggggcggc agcatttcgc aaaggtgatc
    14341 agcacgccat cagcgccttt gatcggcaga tgcccgtggc tttgagtcgc cggtggaatg
    14401 gaggcgtccc catgttgtag atttttcgcg accaccacgc tcattgcgtt accaaggccg
    14461 atttctgcca gcagatcgtc aagcgttgcc agcttcatgc gatccagctc gcgctgaata
    14521 ttttcctgcg ggatttcatt gagcttacgg ctaccaccca aagcatggtt gagcagacga
    14581 cggcccaggc ttacagaatc atcacgcttg aggtttttca gcaactgacg aattttggcg
    14641 cgcgctttcg agctaacgac aaagttcagc caagcggcat tcgggcgagc gcccggagcg
    14701 gtaatgattt caacggtctg accgctagta agcggctgcg acagcgggta aggctggcgg
    14761 tcgacgcgtg cgcccacgca ggcatggccg atatcggtat gcactgcata agcgaagtcg
    14821 acgggcgttg caccggcagg cagctcgaca atgcgccctt ccggtgtgaa aacgtaaatc
    14881 tcatccggga agagatcgga tttaacgctc tcgataaatt caaacgaact accggcgctc
    14941 tgttgcagct ccagcaggct ttgcatccag cgctgggcgc ggatttgtgc ggtagtactg
    15001 gtttcgccgt gctctttata agcccagtgc gcggcaacac ccatctccgc catctgatcc
    15061 atatcttcgg tacggatctg gacctcaacc ggcacgccgt gcgggccaat catcgaggtg
    15121 tgcaacgact gatagccgtt cgcttttgga atggcgatat agtctttcac gcggcccgga
    15181 cgcggcttgt acaggctgtg catctggccc agcacgcgat aacaggtgtc agaatcattg
    15241 acgatcacgc ggaaagcgta gatgtccatg attgagtgaa aacgctgctc tttgagcacc
    15301 attttacagt aaatcgaata aagatgcttt tcgcgaccac tgacgcggca cggtattccc
    15361 gcttcctgca aacgcccttc gatttcagaa agaatcttct ggatcatctc tttacggttg
    15421 ccgcgcgcgg ctttcaccac ttctttgatt acgcgataac ggttgggata cagcgcctca
    15481 aaacccagct cttcgagttc ggttttaatg tggtggatac ctaaacggtg cgccagcggg
    15541 ctgtaaattt cgagagtttc acgggcgatg cggcgacgtt tgtccgggcg aagtgagccc
    15601 agcgtgcgca tgttgtgggt acggtcggca agtttgatga ggatgacgcg gatatcctgc
    15661 accatcgcca taatcatctt gcgaaagttt tcggcctgcg cctctttctt atcgcggaac
    15721 ttgagtttat caagtttcga caccccctct accagctcgg cgacgctttt accaaaaagc
    15781 tgttccatat cctggtaggt ggcgggagta tcttcaatca cgtcatgcag cagcgccgcc
    15841 atcagcgttt catagtcgag tttcatctcg gccagaatgc aggcaaccgc taccgggtgc
    15901 gtgatatagg gttcaccgct tgaacgtgtt tgcccctcgt gagcatcacg tgcaacgaga
    15961 tacgcctgcc gcagacgctt gatttggtct tccggcaggt aggtttgaat cagttgattc
    16021 aggctttcaa acagatacaa gggcgacccg ctttgtgatt aacgacgacc ttcagcaata
    16081 gcggtaacgg cttgtaattc agcggcttcc tgctcttgct gttcctggcg ttcgcgaacg
    16141 tcgaggatct ggttgttgat cagaccttct tcgatttcgc gcagcgcgat tacagtggtt
    16201 ttatcgtttt cttccggtac cagcggatcc tttccgccta cctgcatctg acgagcgcga
    16261 cgcgcggcga ccagtaccag gtcaaaacgg ttaccaattt tctctacagc gtcctgaaca
    16321 gttacgcgtg ccatacttaa aaagctccac aggtgaagaa atgactgggc atgatactga
    16381 aatcaggttc agtctgccaa caatttgctg attaaagcgt catgacgctg cttttggcgg
    16441 ctcatgcgca gacgttcggc gcgaataatg gtcttcaaat cggtcaacgc ggtatcgaag
    16501 tcatcattca caatcaggta atcatattcg gcgtaatggc tcatttctgc aacagcttgc
    16561 gccatacgct ttgcaatgac ctcttcgctg tcctgatcgc gaccgcgtag acggcggtcc
    16621 agttcaattt tagacggcgg taaaataaag atactccgcg cgtgcggcat cttctggcga
    16681 atttgctgcg cgccctgcca gtcgatatcg agaaaaacat cgacaccggt cgccagtact
    16741 tgctcaatgg cctcacgcga agtgccatag taattaccaa aaacttctgc gtgttcgagg
    16801 aacgcatctc tgctaatcat ttctttaaat tcatcatgat taacaaagaa ataatgttca
    16861 ccgtggactt caccaggacg cggttggcgt gtggtgtgtg aaacagaaac ctgggtgtca
    16921 tacaacggtt gggtttttaa taaagcctga atcaggctgg atttacccgc gccactgggg
    16981 gcagaaacaa tataaagcgt gccttgagcc atgagtatct ttcgtatgtg attagcgaaa
    17041 taaagcctac atacgagctt attatacacg gcgcggccat gtgacgtagc ccttgctcac
    17101 actttttgcg caaagttgtt gcgttttgcg tagctgtttc cgcatttgcc tgtgatttac
    17161 tgcaataaag acgattctca ggaagcgtcc tcgcagactc cgtttttgcc actcggcttt
    17221 gccttcacca cgttgtatat ctgccagcct gactacagcg gaggtgaaga tgaaagtatg
    17281 gatggcgata ttaataagta tcttgtgctg gcaatcatct gtgtgggcgg tctgtccggc
    17341 ctggtcgcca gccagagcac aggaagaaat tttccgcctg caacagcaaa taaaacagtg
    17401 ggacgatgac tactggaagg aaggaaaaag tgaggtggaa gacggtgttt acgatcagtt
    17461 aagcgcccgt cttacgcagt ggcaacgctg ctttgtgagc gagccccgcg acgtgatgat
    17521 gccgccttta aatggtgcgg ttatgcatcc ggttgcccat acgggtgtac gcaaaatggc
    17581 ggataaaaac gcattaagtc tgtggatgcg agaacgtagc gatctttggg tgcagccaaa
    17641 agttgatggc gttgcggtaa ccctggttta tcgggacggg aaactgaaca aagcaatcag
    17701 tcgcggtaat ggcctgaaag gcgaggactg gacgcagaaa gttagcttaa tttccgctgt
    17761 gccgcaaacc gttagcgggc ctttagccaa cagtacgctt cagggggaaa tatttctcca
    17821 gcgcgagggg catatccaac aacaaatggg gggaataaat gcccgcgcaa aagttgctgg
    17881 cctgatgatg cgccaggacg atagcgacac gctgaattct ctgggcgttt ttgtctgggc
    17941 atggccggat ggaccgcagt taatgaccga tcgtttaaag gagctggcta ccgcaggttt
    18001 tactctaacg cagaggtata cccgtgcggt taaaaatgct gatgaggttg cgcgcgtacg
    18061 caatgagtgg tggaaagcga aattaccctt cgtcaccgat ggcgtggttg tgcgaggggc
    18121 gaaagaaccc gaatcccgcc attggctacc aggccaggca gaatggctgg tggcctggaa
    18181 atatcaacct gtagctcagg ttgccaaagt gaaggcgatt cagtttgcgg tgggtaagag
    18241 cggtaaaata tcggtggttg cgtcactcgc acctgtcatg ctggatgata aaaaagtcca
    18301 gcgggtgaat attggttccg tcaggcgctg gcaggagtgg gatattgcgc ctggtgatca
    18361 gatcctcgtc agccttgccg gtcagggtat tcctcgcatt gatgatgtgg tctggcgcgg
    18421 tgcagaacgt acaaaaccga caccaccaga aaaccgcttt aactcgttga cctgctactt
    18481 tgcttctgat gtttgtcagg aacagttcat ttcacgctta gtctggctgg ggtcaaaaca
    18541 ggttctgggg ctggatggca ttggtgaggc cggctggcgc gcgctgcatc agactcatcg
    18601 ctttgagcat atcttttcct ggcttttatt aacgccagag caattacaga acacgccggg
    18661 gatcgcgaaa agtaaaagtg cgcagctatg gcatcggttt aatctggctc gtaagcagcc
    18721 ttttactcgc tgggtgatgg caatgggaat accgctaacc cgggcggcgc ttaatgccag
    18781 tgatgaacgg tcctggtcgc aacttttatt tagcacggag cagttctggc agcagctgcc
    18841 ggggactgga tcggggcgcg ccagacaggt tattgaatgg aaggaaaatg cgcaaatcaa
    18901 gaagctgggc agttggctgg ctgcccagca gatcacaggt tttgaacctt agtgtccttc
    18961 gtggctgtag taaaagaccg gtaatcccag cttaaggcgt agcgccagta agcgggcaaa
    19021 aaagccgaat accagggtgg agataattac cacatcatgg ttagaaacat agtgttgcag
    19081 cgcgatgtac agcacggcgg aggcaaatga gacaccggca tacaactctt tctggaatac
    19141 cagtgggata cgtttacaga acatatcgcg caaaacgccg ccaaacacgc cggtggttac
    19201 cgccgcgaca acggcaataa ttgggccgtg ccccatatcc agtgcaacct gtgcgccgat
    19261 gatagaaaag accaccagtc caagcgcatc gagcaccaga aacactttgc gcaggtaagg
    19321 cattacagga gcaacgatag tggtgagtac tgcggcggta gcgacgataa tcacatattc
    19381 cgggtgtttg acccagccga gcggatagtg gcccagcaga atatcgcgca ctgaccctcc
    19441 gccaattgcg gtggcggtcg caataataat tacgccaaat gtatccatgc ggcgtcgccc
    19501 ggccgccaga gcccccgtca tggcttcggc agtaatgcca accaaataca aaatgtgtaa
    19561 cagcatggtt ctctccctaa aaatctgtct ggcagggtag cgatttgtgc gtattgtcac
    19621 gattgagatt ttttaaggtg agaggaaatt gataacttgt agttatgtga aatcgctatt
    19681 ttctgtagcg tagatgcatt cttctgactt cttgtctgag aaaatgcata agaaaaaata
    19741 ggttaaaatt gaggggtgga aaagaaaggt gcaatgttta gccattgtgt tcaccgcacc
    19801 tggactttgg cttacgcatc gggtatctca atcaacaaaa agagttttta gtttctgcta
    19861 tttcttctct gtaattttta cactgttttc caactgcttg atgctgacct gcggggtcgg
    19921 caactcctca ggcatggttc cgccaagttc ctgaatggtt tgcctcacct tgctacccac
    19981 atcaaagtgt gtggtattag cttgctgttt tgaattcacg ccatcccgct tgagtttttc
    20041 ttctgtttgg gtagctcgaa agagattagc cgccagttct gttgaaccca tatgatccag
    20101 gatcttctga ctctttttca gccccttcag ttggtggatg gctttctgat ctaatccacc
    20161 atacagcccc tggtaaccat gattctgaaa tatggcgaaa tcagtagctg tcgctaccgc
    20221 agcttgctgt gctgcctcaa ccaattgttt gttatgttct ttcaactcat ttctcagaaa
    20281 cagacgcttt tcgtcttcac gaagttgctt aaatgcttca tcgtcagcaa gctcctgccg
    20341 tcgggtctgg atagcaaaat aagtctgccc tgccgcaatg accggtttcg cagggtcgcc
    20401 gttttgtact accaggtaac aggcatagcg ggagagatga acatcttcaa gttctcgttg
    20461 agcccctgaa cctaatacaa ccattttggt ggtttcaacg aaatggtcag atgcagcttg
    20521 attgctggct tcgcacgctt gagtcgcgcg ggccagtacc ttttgaaaat tgcgccagtc
    20581 ccgataatcc agtaacggtg ccaattctct agctgaccaa aattcttgcc cttcagtgcc
    20641 ataatgccgt atttcttcaa aaggttgatg atgttcgttc attgcccact ccatgtgata
    20701 tcgattgcaa agaaagtaaa tctgtaactg tatttatata cagttgtgag tatctcacgt
    20761 tctcagtgtg ctgggcaaaa aaacatcaca aaatctggag caggcacgca aaacagaatt
    20821 gaagttgcgg ggaagaaaaa tgccgctctg tttgagtggg cggcatttgt gaaggctgaa
    20881 aagtaagaat tggcgttacg agttattcaa tgttctgaat ctgctcgcgc atctgctcaa
    20941 tcaacacttt cagctcaatc gcggagtttg tcacttcggc attgatagac ttcgacgcaa
    21001 gagtgttcga ctcgcggttg aattcctgca tcataaagtc cagacgacga ccaacggctt
    21061 cttttttctt cagaatgttg taggtctctt tgacatgcgc ttccaggcgg tccagttctt
    21121 cggcaacgtc aattcgttgt gccagcagaa ccagctcctg ctccagacgg ttgttttcca
    21181 actgaacctg agcatcttcc agcttcgcga ccagacgctc acgctgccat tgcaggattt
    21241 ccggcatatg ggcgcggact ttgaccactt cggcggtgac gccttccaga cgctgctcga
    21301 tcaatgcttt cagtgcctga ccttcggttt cgcgcgcgac aataaagtcg tccagcgtac
    21361 catccagcgc cgcgagaatt tcagcggcaa tggcgtcaag atcctgctcc tgggctgcca
    21421 tcacgcccgg ccagcgtaga atatcaaccg ggttgatttc cccttcgtca ctctgcattt
    21481 ttacccagtt cgcggcagtt accagctgtt tagccagttt ttcgttgagg atcagctcac
    21541 cttgcgcgct aacatctggc tcatagcgca gggtacattc cactttaccg cgcgtcaggc
    21601 gagaacgaat acgctcgcga acgacaggtt caaggctacg gaactgctcc ggcagacgaa
    21661 agtaagtttc cagataacgc tggtttaccg agcgcatttc ccaggttgcg ctcccccatt
    21721 cacccttgat ttcacgccgg gcgtaggcgg tcatactgcg gatcatagac gttcctgttt
    21781 ataaaaggag aggtggaagg attatagcca tcgatgcctt gtcaggatag gaataaccgc
    21841 cggaagtccg tataatgcgc agccacattt gtttcaagcc ggagatttca atatgcgtcc
    21901 agcaggccgt agcaataatc aggtgcgtcc cgttaccctg actcgtaact atacaaaaca
    21961 tgcagaaggc tcggtgctgg tcgaatttgg cgataccaaa gtgttgtgta ccgcctctat
    22021 tgaagaaggc gtgccgcgct tcctgaaagg tcagggccag ggctggatca ccgcagagta
    22081 cggcatgctg ccacgttcta cccacacccg taacgctcgt gaagcggcga aaggtaagca
    22141 gggtggacgc acaatggaaa tccagcgtct gatcgcccgt gctcttcgcg cggcagtaga
    22201 tttgaaagcg ctgggtgagt tcaccattac gctggactgc gacgtgcttc aggctgatgg
    22261 tggcacgcgt accgcgtcga ttacgggtgc ctgcgtggcg ctggcagatg cgctacagaa
    22321 gctggtggaa aacggcaagc tgaaaaccaa cccgatgaaa gggatggtag ccgcagtttc
    22381 tgtcggaatt gtgaacggcg aagcggtttg cgatctggaa tacgttgaag actctgccgc
    22441 agagaccgac atgaacgtag tgatgaccga agacgggcgc atcattgaag tgcaggggac
    22501 ggcagaaggc gagccgttca cccatgaaga gctactcacc ttgttggctc tggcccgagg
    22561 gggaatcgaa tccattgtag cgacgcagaa ggcggcgctg gcaaactgat ttttaaggcg
    22621 actgatgagt cgcctttttt ttgtctgtag aaaagtaaga tgaggagcga aggcatgaaa
    22681 ccatatcagc gccagtttat tgaatttgcg cttggcaagc aggtgttaaa gtttggcgag
    22741 tttacgttga agtccgggcg caaaagcccc tatttcttca acgccgggct gtttaatacc
    22801 gggcgcgatc tggcactgtt aggccgtttt tacgctgaag cgttggtgga ttccggcctt
    22861 gagttcgatc tgctgtttgg ccctgcttac aaagggatcc cgattgccac cactaccgcc
    22921 gtggcgctgg cggagcatca tgaccttgac ctgccgtact gctttaaccg caaagaggca
    22981 aaagaccacg gtgaaggcgg caatctggtt ggtagcgcgt tacaaggacg cgtaatgctg
    23041 gtagatgatg tgatcaccgc cggaacggcg attcgcgaat cgatggagat tattcaggct
    23101 aatggcgcga cgcttgctgg cgtgttgatt tcgcttgatc gtcaggaacg ggggcgcggc
    23161 gagatttcgg cgattcagga agttgagcgt gattacaact gcaaagtgat ctctatcatc
    23221 accctgaaag acctgattgc ttacctggaa gagaagccgg aaatggcgga acatctggcg
    23281 gcggttaagg cctatcgcga agagtttggc gtttaaagaa actcgccgga tgaaaagtca
    23341 tccggcgtca tattactgca actgtgccgc aattagcggc cagcgggcgt caaaatcatc
    23401 cgtcgggcgg tatttaaatt cgctgcggac aaaacgtgac agcatacctt cacagaaggc
    23461 taggatctgg cttgccagca gggtttcatc ggtggtgtaa ccttcaccct cacgcattct
    23521 cttttcacgc aatacctgac gcagctgcgc ttcaatacgc tcgaacagct ggttgatgcg
    23581 cccttgcagg cgatcctgtt caaacattag cgcatgacca gtgaggatgc gggtcaggcc
    23641 aggattacgc tcaccaaaac cgagaagcag caacacaatc agacgcaggc gcgctgtggt
    23701 gtctttctca tctttcagaa tcagattgat gcgagtaatc aggctatctt cgataaactc
    23761 aatcaggcta tcgaacatgc gggtcttact ggggaagtgg cgatacagtg ccgcttcgga
    23821 aacgccgaca gaggcggcca gttttgccgt cgtgatacgt tggcttccat cgctggattc
    23881 cagcatcagc gccagagact gaagtatttc ctcgcgacgg ttccttttcg cagtttgttt
    23941 ttctgccatg ttacaaaata cccctgaaaa taagcacttg ccaggcggca cccacgctat
    24001 gaccgcaaac gaaatgttta cggctatgtt atgacgttat tcggatgcgt atgtgttact
    24061 gacgaccaga gtgaccaaag ccgccttcac cgcggtcggt ggcgtcgaaa tcttccacca
    24121 gattaaattc agcctgtact accggaacaa aaatcatctg ggcgatacgt tcgccaggtt
    24181 gaatggtgaa gctgtcctga ccacggttcc acacggaaat catcaactgg ccctgatagt
    24241 cggaatcgat caatcctacc aggttaccaa gcacgatacc gtgcttatgt cccaatccgg
    24301 agcgcggcag catcattgcc gccagtgaag gatcggcaat atgaatcgcc agcccggtcg
    24361 gaaccagcgt agtgtcaccc ggagccagtt ctacggcgtc gtcgagacag gcacgcaggt
    24421 caagtccggc agagccagag gtggcataag tcgggagcgg aaattccttc ccaacgcgcg
    24481 ggtccagaat cttaacgtcg atttttttca tcataacggg tcacgatctc gtcgagtaat
    24541 aattggccaa ggagctcttt gcgctcaagc ggtaagactt tatctccgtc ctgccagaaa
    24601 aggtgtaatg cgttgttgtc gctgttaaat ccttgagttg gctgggaaac atcgttcgcg
    24661 cagatcagat caaggttttt acggatacgt ttttgccggg cgtattcttc cacattattt
    24721 gtttcggcgg caaatccaac gacgtagggt cgatggtctt ttagtgcggc aacgcctgcg
    24781 acgatatcgg ggtttttaac catttttatt gttaattcat caccctgcgt ggcctgcttt
    24841 ttgattttct ctggggccac ggtagctgcg cgataatccg ccacggcggc gcagccgata
    24901 aaaatatttt gctgctgtac agaagcattc acggcggctt ccatttccag cgcggtcatc
    24961 acatcaacac gtttaacaaa cggtggcgtc ggtagtgaaa ccggacctga taccagcgtg
    25021 acgttcgcgc cacgacgggc agcggcggcg gcgatagcaa aacccatctt gccggagctg
    25081 tgattagaga tataacgcac gggatcgagc ggttcacgcg tcgggccggc ggtaatcata
    25141 atgttcagat gtttcaggtc gttgacgggc gaaaaatgcg ctaccgccat atccacaatg
    25201 gttaacggat cgagcattcg cccaggaccg atatcaccac aagcctgact gccactgtct
    25261 ggcccccaga tgagcaaacc acgggaagca agcacctcta aattatgctg cgtggcagcg
    25321 gcacggtaca tctgctggtt catggcgggg agcacggcta caggcgcagg tgtagccaga
    25381 caaatcgtcg ataccaggtc attcgccatt ccggcagcaa cacgggcaat caaatctgcc
    25441 gtggcagggg cgagaatcac taaatcagcc catttaccca gctcaatatg gcccatagcg
    25501 gcttcggctg ccgggtccag caaactgtcg gaaacgggat aaccagaaac cgcctgcaag
    25561 ctaagtgggg tgataaaggc ttttgccgct tcggtcatgg ctacgcggac gtcggccccg
    25621 cgatcgcgca aacgacgcac cagttcaggg gttttatagg cagcaatacc gccgctaacg
    25681 ccgagaacga tttttttacc ggccaggctc atgatgattt ttcctgttgg gtgacaccag
    25741 aagttggcga ttttatcaca atacttttgt tgtcgtgcct tcacctgaga ttcactttgc
    25801 gaggcgcttt ccaggattga aaactggccg tcgatttaac ggaacggcta tgacaggatg
    25861 cgagcaccac aaaggaggtg aaggtgaaaa acaatgcaca gctgttgatg ccgcgcgaaa
    25921 aaatgctgaa gtttggtatt agcgccttaa ctgatgtcga gctgctggcg ctatttctgc
    25981 gtaccggaac gcgcggtaaa gatgtattaa ccctggcaaa agagatgctg gagaatttcg
    26041 gctctcttta tggcttgtta acctctgaat atgaacaatt tagtggtgtt catggaattg
    26101 gcgtagcgaa atttgcccag ttaaagggga ttgctgaact ggcgcgacgt tactacaacg
    26161 tgcggatgcg tgaagaaagc cctttactca gcccggagat gacgcgggaa tttttacaaa
    26221 gccagctcac gggtgaggag cgggagatct ttatggtgat ctttctcgac tcccaacacc
    26281 gggttataac gcatagccgt cttttttccg gcacgctaaa ccatgttgaa gtccatcctc
    26341 gggaaattat ccgcgaagcg ataaaaataa acgcctcggc gctgatcctt gcacataatc
    26401 acccttcggg ttgtgctgaa cccagtaaag cggataaact cattactgaa cggataataa
    26461 agagttgtca gttcatggat ttacgcgtgc tcgaccatat cgtgattggg cgtggagagt
    26521 atgtttcttt tgccgaacgc ggctggattt aacccgctat gcgcgatcct tcgggatctt
    26581 tgtctgttcg ggacttgagc acatcgctga gtcagcgtat actacgccac ctttgagaat
    26641 ctcgggtttg gcatttgggc ctggcaatcg agagttcaca tagaactgcg atgaccgggc
    26701 tgtaaagcct gacgaggcgc caatacccca tacgaagctc gagctaattt gatttttgga
    26761 gaatagacat gtcccgagtc tgccaagtta ctggcaagcg tccggtgacc ggtaacaacc
    26821 gttcccacgc actgaacgcg actaaacgcc gtttcctgcc gaacctgcac tctcaccgtt
    26881 tctgggttga gagcgagaag cgttttgtca ccctgcgcgt atctgctaaa ggtatgcgtg
    26941 taatcgataa aaaaggcatc gatacagttc tggctgaact gcgtgcccgt ggcgaaaagt
    27001 actaagtact tagaggaaat aaatcatggc taaaggtatt cgtgagaaaa tcaagctggt
    27061 ttcttctgct ggtactggtc acttctatac cactacgaag aacaaacgta ctaagccgga
    27121 aaaactggaa ctgaaaaaat tcgatccagt tgttcgccag cacgtgatct acaaagaagc
    27181 gaaaatcaaa taattctcgc tttgatgtaa caaaaaacct cgctccggcg gggttttttg
    27241 ttatctgctt gcccccatat tgactgcatc tgttcattcc tggagatgct atgcctgaat
    27301 tacccgaagt tgaaaccagc cgccgcggca tagaaccgca tctcgttggt gcaaccattc
    27361 ttcatgcagt ggtgcgcaac ggacgcttgc gctggccggt ttcagaagag atctaccgtt
    27421 taagcgacca accagtgctt agcgtgcagc ggcgggctaa atatctgctg ctggagctgc
    27481 ctgagggctg gattatcatt catttaggga tgtctggcag cctgcgcatc cttccagaag
    27541 aacttccccc tgaaaagcat gaccatgtgg atttggtgat gagcaacggc aaagtgctgc
    27601 gctacaccga tccgcgccgc tttggtgcct ggctgtggac caaagagctg gaagggcata
    27661 atgtgctggc ccatcttgga ccggagccgc ttagcgacga tttcaatggt gagtatctgc
    27721 atcagaagtg cgcgaagaaa aaaacggcga ttaaaccgtg gctgatggat aacaagctgg
    27781 tggtaggggt agggaatatc tatgccagcg aatcactgtt tgcggcgggg atccatccgg
    27841 atcggctggc gtcatcactg tcgctggcag agtgtgaatt gttagctcgg gtgattaaag
    27901 cggtgttgct gcgttcgatt gagcagggtg gtacaacgct gaaagatttt ctgcaaagtg
    27961 atggtaaacc gggctatttc gctcaggaat tgcaggttta cgggcgaaaa ggtgagccgt
    28021 gtcgggtgtg cggtacgccg attgtggcga ctaaacatgc gcagcgggca acgttttatt
    28081 gtcggcagtg ccagaagtaa ttcatgcgcg ccggatggca taccatccgg cataaacgct
    28141 acgctaactt cgccatcagc gcctgatgga cattctccgg caggaaatgg gtgacatcgc
    28201 cctgatggcg cgccacctct ttcaccaacg atgaagagat aaacgaccac tctttcgacg
    28261 gcatcagaaa cacactttcc agttccggca ttaagtggcg attcatatgc gccagctgca
    28321 tttcatattc aaaatctgcc accgcacgca ggccacgaat cagcaccgta gcgtgttgat
    28381 tacgggcgaa gttcgccatc aaatcactaa acccgaccac ttccacgttc cccagatgcg
    28441 cggttgcctg ctgtgccagt gccacacgct cttccagggt aaacatcggt tttttactgg
    28501 ggctggcggc aatcgccaga ataacgtgat cgaacatctg cgtggcgcgc gtcacgatat
    28561 cgatatgacc attggtaatg ggatcgaaag tacccggata aatcgcccgt ttttgcataa
    28621 caacctcaat gcgttttcgg tggcaggtaa ggttccagca gttgaagcag acgctgtagc
    28681 gcgccctggt tttgatacag tacttcaacg gcatgacggc catagaaact acggtaatcg
    28741 gcgtcggtga gtaaagagga aacctctttt gcaagcgtag tggcatcggt aacggtaatc
    28801 agcccgcttg cctgctccag ccgcgcgcaa atgtctttaa agttaaaagt atgcggcccc
    28861 atcaataccg gaattgcgtg tgcggcagct tccagcggat tatgcccacc acgttcaacc
    28921 agtgaaccgc caacaaaggc gagatcggca atgccgtaca gtaacatcaa ctcgcccatc
    28981 gtatcgccaa ccacaacctg cgtgctggtg gaggggactt cccctgaaga gcgtgtgata
    29041 tagcttagtc cagcctggcg gacaaggtta atcgcatccg ggaagcgttc tggatgacgg
    29101 ggtaccagga tgagcaataa attcgggaat tgctgtaata aagcctgatg tgcggcaatg
    29161 accacgctct cttcaccttc gtgagtgctg gtggcaatcc ataccgggcg gtgtggtgcc
    29221 cactggcggc gcagcgtcac ggctttagca gccaactgcg gcgttacaga aatatcgaat
    29281 ttcaggctac cggtaacggt tacctggtta tttttcgcgc ccagcgccac aaaacgtgca
    29341 ccatcttctt cattttgggc ggcaatcagc gtaatacgac gcagcaagcg acggacgaat
    29401 ttacccagtt tggcataacc tgcggccgag cgggcagaga gtcgcgcgtt agcgatcacc
    29461 agcggaattt tacgtttatg tagcgccgca atcaggttag gccatagttc ggtttccata
    29521 atcaacacca gtttagggtc gactttattc aggaaacggt tgagtgcatc gggcagatca
    29581 tacggcagat aaacgtgctg aacatccttc ccgaaagccg attgtacgcg ctccgaaccg
    29641 gttggcgtca tggttgttac ggtaataggt aaatcaggat aacgatgacg cagcgcgcgc
    29701 accaacggga tcgccgccag agtttcaccg acggagacgg agtgcagcat aatgccgcct
    29761 ggttttagcg gatggcggta aaaaccgtaa cgttcacccc agcgttttcg ataggccgga
    29821 gccttacgtc cgcgcaccca gagccgtatc cagatcagcg gctgaataag gtagagaagg
    29881 gcggtgtaaa gcaattcgag catagtaaat agctgactta tggatgtgct ggggattcta
    29941 tgtatttagc tgtggcttta ccattacttt tcccgttttt gacttaaata gcaatttacc
    30001 cgccaattct tgctgaacat ctatactttt tttgtatttt tatgcgattc attgatttct
    30061 gctgtacgtg caggaaggaa tcgcagagca acagtgaaag aatatggttg ctaatgattt
    30121 tatttgattg atttctttat tgaaaatcaa caatgtgctt aaaaatcatt cgggtcggat
    30181 tgccgcgaaa gaagtgatac actagcgcgt caatatcagt gcggtatcag tattcaggta
    30241 gctgttgagc ctggggcggt agcgtgcttt ttctgcttca ccttaatcgg ataatctcaa
    30301 caaaaagagt tacttgtgga taagccattt cgaagaattt tgctcattaa gatgcgtttt
    30361 catggggata tgttattaac tactcccgtc attagttcgc tgaaaaaaaa ttaccctgac
    30421 gcaaaaatcg atgtgctgct ttatcaggac accatcccga tcctgtctga aaatccagag
    30481 attaacgcgc tctacggcat aaaaaataaa aaagcaaaag cctcagaaaa aattgccaac
    30541 ttttttcatc tcatcaaggt attacgtgcc aataagtatg accttatcgt caatcttacc
    30601 gatcaatgga tggttgctat actggttcgc ttattaaatg cccgtgtgaa aatttcccag
    30661 gattatcatc atcggcagtc tgctttttgg cgtaaaagtt tcacccattt ggtgccgttg
    30721 cagggtggaa atgtggtgga aagtaactta tccgtgctga caccattggg acttgattcg
    30781 ttggtgaagc agacaaccat gagttacccg cctgcaagct ggaaacgtat gcgtcgcgaa
    30841 cttgatcacg ctggtgttgg acaaaattat gtggttatcc aacctacggc gcggcaaatc
    30901 ttcaaatgct gggacaacgc caagttttcc gctgtgattg atgccttaca tgctcgtggt
    30961 tatgaagtcg ttctgacgtc cggcccagat aaagacgatc tggcctgcgt caatgaaatt
    31021 gcgcaaggat gccagacgcc accagtaacg gcgctggctg gaaaggtgac cttcccggaa
    31081 cttggtgcgt taatcgatca tgcgcagctg tttattggcg ttgattccgc accggcgcat
    31141 attgccgctg cagttaatac gccgctgata tcgctgtttg gtgcgacaga ccatattttc
    31201 tggcgtcctt ggtcaaataa catgattcaa ttctgggcgg gagattaccg ggaaatgcca
    31261 acgcgcgatc agcgtgaccg aaatgagatg tatctttcgg ttattccggc ggcagatgtc
    31321 attgctgctg tcgataaatt actgccctcc tccacgacag gtacgtcgtt atgatcgttg
    31381 ctttttgttt atataaatat tttccctttg gcggtttgca gcgcgatttt atgcgtattg
    31441 ctcagacagt cgccgcccga ggtcatcatg ttcgggttta tacccagtcg tgggaaggcg
    31501 aatgccctga tgtatttgaa ctgatcaaag tgccggttaa atcgcatacc aatcacgggc
    31561 gcaatgcgga gtattttgcc tgggtgcaaa aacatttacg cgaacatccc gtcgataaag
    31621 tcgttggatt caacaaaatg ccggggctgg acgtttatta tgccgctgat gtttgttatg
    31681 ccgagaaagt cgcgcaggaa aaaggctttt tctatcacct gacgtcacgt tatcgccatt
    31741 atgccgcctt tgagcgggca accttcgaac agggcaagcc gacacagctg ctgatgctga
    31801 cagataagca aatcgccgat ttccagaaac attatcagac tgaagcggag cgttttcata
    31861 ttctaccacc ggggatttat cctgatcgta aatatagcca gcagccagct aatagccgtg
    31921 aaatcttccg taagaagaat ggaataaccg aacaacaata tttattgttg caggtcggtt
    31981 cagacttcac gcgtaaaggt gtcgatcgtt ccattgaagc acttgcttcg ttaccggatt
    32041 cgctgcgcca caacacattg ctatatgttg ttggacagga taaaccgcga aaatttgagg
    32101 cgctggcaga aaaacgcggc gtgcgcagta atgttcactt cttctcgggg cgcaacgatg
    32161 tctcggaatt aatggcggcg gcggatttat tactgcatcc tgcctaccag gaagcagcgg
    32221 gaattgtgct actggaagcg attactgcag gattaccggt actaacaaca gccgtttgtg
    32281 gctatgcgca ttatattgtc gacgccaatt gcggcgaggc tattgctgag ccattccgcc
    32341 aggaaacatt gaatgagatt ttacgcaaag cgttaacgca atcttcattg cgccaggcct
    32401 gggcggaaaa tgcgcgacat tatgctgata cacaagattt atacagtctg ccagagaaag
    32461 cggcggatat cataacgggt ggtctggatg gttgaactta aagagccgtt tgccacgtta
    32521 tggcgcggta aagatccttt tgaggaagtt aaaaccttgc agggtgaggt ttttcgtgaa
    32581 ctggaaactc gccgcactct gcgatttgaa atggcgggca aaagctattt tctcaaatgg
    32641 catcgcggca cgaccctgaa agagataatc aaaaatttac tctcattgcg gatgccagta
    32701 ttaggcgcag accgcgaatg gaatgcgatt catcgactgc gggatgtcgg cgttgatact
    32761 atgtatgggg tggcatttgg cgaaaaaggc atgaatccgc tgaccagaac ttcatttatt
    32821 attaccgaag atctgacacc aaccataagt cttgaagatt actgtgctga ctgggcgact
    32881 aaccctccag atgttcgcgt aaagcgtatg cttattaagc gtgtcgcgac gatggtgcac
    32941 gatatgcatg ctgcgggcat taaccaccgt gactgttata tctgtcattt cctgctgcac
    33001 ttgccttttt ccggtaagga agaggagtta aaaatttcgg taattgacct gcaccgggcg
    33061 cagcttcgca cgcgcgttcc acgtcgttgg cgggataaag atcttattgg gctttatttt
    33121 tcttcgatga atatcggcct gactcagcgg gatatctggc ggtttatgaa agtgtatttt
    33181 gccgccccgc ttaaagacat tctcaagcag gaacaaggac tgctgtcgca agcagaagca
    33241 aaagccacaa aaatcaggga aagaacgatt cgaaaatcgt tgtaacccgt aacttatttt
    33301 tgccaaaatt ttggatacag aataaatatg tctcaactca atgatagtga catcatcctt
    33361 tttgagtata attttcatta tcaaaatata agatctaaaa atactcttga tatagcattt
    33421 ggtattgaca gaaattttct tttt
//
LOCUS       DAZKIF010000043        32587 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZKIF010000043 DAZKIF010000000
VERSION     DAZKIF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.043"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            203..1123
                     /gene="cbpA"
                     /locus_tag="ACHOTW_002011"
     CDS             203..1123
                     /gene="cbpA"
                     /locus_tag="ACHOTW_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP9844123.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1123..1428
                     /gene="cbpM"
                     /locus_tag="ACHOTW_002012"
     CDS             1123..1428
                     /gene="cbpM"
                     /locus_tag="ACHOTW_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP9844124.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1582..2181)
                     /gene="torD"
                     /locus_tag="ACHOTW_002013"
     CDS             complement(1582..2181)
                     /gene="torD"
                     /locus_tag="ACHOTW_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP9844125.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(2178..4724)
                     /gene="torA"
                     /locus_tag="ACHOTW_002014"
     CDS             complement(2178..4724)
                     /gene="torA"
                     /locus_tag="ACHOTW_002014"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP9844126.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(4724..5896)
                     /gene="torC"
                     /locus_tag="ACHOTW_002015"
     CDS             complement(4724..5896)
                     /gene="torC"
                     /locus_tag="ACHOTW_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP9844127.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6026..6718
                     /gene="torR"
                     /locus_tag="ACHOTW_002016"
     CDS             6026..6718
                     /gene="torR"
                     /locus_tag="ACHOTW_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP9844128.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(6691..7719)
                     /gene="torT"
                     /locus_tag="ACHOTW_002017"
     CDS             complement(6691..7719)
                     /gene="torT"
                     /locus_tag="ACHOTW_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP9844129.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            7802..10546
                     /gene="torS"
                     /locus_tag="ACHOTW_002018"
     CDS             7802..10546
                     /gene="torS"
                     /locus_tag="ACHOTW_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFP9844130.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            10618..11691
                     /gene="yccM"
                     /locus_tag="ACHOTW_002019"
     CDS             10618..11691
                     /gene="yccM"
                     /locus_tag="ACHOTW_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9844131.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(11740..11874)
                     /locus_tag="ACHOTW_002020"
     CDS             complement(11740..11874)
                     /locus_tag="ACHOTW_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP9844132.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(11902..12132)
                     /gene="ymcE"
                     /locus_tag="ACHOTW_002021"
     CDS             complement(11902..12132)
                     /gene="ymcE"
                     /locus_tag="ACHOTW_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP9844133.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(12107..12295)
                     /gene="ymcF"
                     /locus_tag="ACHOTW_002022"
     CDS             complement(12107..12295)
                     /gene="ymcF"
                     /locus_tag="ACHOTW_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9844134.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(12306..12518)
                     /gene="cspG"
                     /locus_tag="ACHOTW_002023"
     CDS             complement(12306..12518)
                     /gene="cspG"
                     /locus_tag="ACHOTW_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP9844135.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            12804..13016
                     /gene="cspH"
                     /locus_tag="ACHOTW_002024"
     CDS             12804..13016
                     /gene="cspH"
                     /locus_tag="ACHOTW_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP9844136.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            13458..13763
                     /locus_tag="ACHOTW_002025"
     CDS             13458..13763
                     /locus_tag="ACHOTW_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844137.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            13870..14514
                     /gene="gfcB"
                     /locus_tag="ACHOTW_002026"
     CDS             13870..14514
                     /gene="gfcB"
                     /locus_tag="ACHOTW_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP9844138.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            14511..15257
                     /gene="gfcC"
                     /locus_tag="ACHOTW_002027"
     CDS             14511..15257
                     /gene="gfcC"
                     /locus_tag="ACHOTW_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9844139.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            15257..17353
                     /gene="gfcD"
                     /locus_tag="ACHOTW_002028"
     CDS             15257..17353
                     /gene="gfcD"
                     /locus_tag="ACHOTW_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9844140.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            17399..18538
                     /gene="gfcE"
                     /locus_tag="ACHOTW_002029"
     CDS             17399..18538
                     /gene="gfcE"
                     /locus_tag="ACHOTW_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP9844141.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            18526..18972
                     /gene="etp"
                     /locus_tag="ACHOTW_002030"
                     /gene_synonym="yccY"
     CDS             18526..18972
                     /gene="etp"
                     /locus_tag="ACHOTW_002030"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP9844142.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            18992..21172
                     /gene="etk"
                     /locus_tag="ACHOTW_002031"
     CDS             18992..21172
                     /gene="etk"
                     /locus_tag="ACHOTW_002031"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP9844143.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(21292..22596)
                     /gene="appA"
                     /locus_tag="ACHOTW_002032"
     CDS             complement(21292..22596)
                     /gene="appA"
                     /locus_tag="ACHOTW_002032"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP9844144.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(22676..22768)
                     /gene="cbdX"
                     /locus_tag="ACHOTW_002033"
     CDS             complement(22676..22768)
                     /gene="cbdX"
                     /locus_tag="ACHOTW_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP9844145.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(22781..23917)
                     /gene="appB"
                     /locus_tag="ACHOTW_002034"
     CDS             complement(22781..23917)
                     /gene="appB"
                     /locus_tag="ACHOTW_002034"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9844146.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(23929..25473)
                     /gene="appC"
                     /locus_tag="ACHOTW_002035"
     CDS             complement(23929..25473)
                     /gene="appC"
                     /locus_tag="ACHOTW_002035"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP9844147.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(25607..26464)
                     /gene="hyaF"
                     /locus_tag="ACHOTW_002036"
     CDS             complement(25607..26464)
                     /gene="hyaF"
                     /locus_tag="ACHOTW_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP9844148.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(26461..26859)
                     /gene="hyaE"
                     /locus_tag="ACHOTW_002037"
     CDS             complement(26461..26859)
                     /gene="hyaE"
                     /locus_tag="ACHOTW_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP9844149.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(26856..27443)
                     /gene="hyaD"
                     /locus_tag="ACHOTW_002038"
     CDS             complement(26856..27443)
                     /gene="hyaD"
                     /locus_tag="ACHOTW_002038"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP9844150.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(27440..28147)
                     /gene="hyaC"
                     /locus_tag="ACHOTW_002039"
     CDS             complement(27440..28147)
                     /gene="hyaC"
                     /locus_tag="ACHOTW_002039"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP9844151.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(28166..29959)
                     /gene="hyaB"
                     /locus_tag="ACHOTW_002040"
     CDS             complement(28166..29959)
                     /gene="hyaB"
                     /locus_tag="ACHOTW_002040"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP9844152.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(29956..31074)
                     /gene="hyaA"
                     /locus_tag="ACHOTW_002041"
     CDS             complement(29956..31074)
                     /gene="hyaA"
                     /locus_tag="ACHOTW_002041"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP9844153.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            31500..31587
                     /locus_tag="ACHOTW_002042"
     tRNA            31500..31587
                     /locus_tag="ACHOTW_002042"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31534..31536,aa:Ser,seq:tga)
     gene            32190..32587
                     /gene="tccP2"
                     /locus_tag="ACHOTW_002043"
                     /pseudo
     CDS             32190..32587
                     /gene="tccP2"
                     /locus_tag="ACHOTW_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
BASE COUNT         7978 a         8605 c         8290 g         7714 t
ORIGIN      
        1 gtttcaacta tcaaaaatcg ctcacccttt ttcacctgtt taaaatatgt tcagcgcccc
       61 accttgaggc gacctcctct ccgcgatgat ttcaataaca tattctgtgt tggcatatga
      121 aattttgagg attaccctac acttatagga gttaccttac aggggttcct tcaatttgtg
      181 ttgatttacg cgagataacg ctatggaatt aaaggattat tacgccatca tgggcgtgaa
      241 accgacggac gatctcaaga caatcaagac cgcctatcgt cgacttgccc gcaaatacca
      301 tcctgatgtc agcaaagaac cggatgccga agcccgcttc aaagaggtcg ctgaagcctg
      361 ggaagtgtta agtgatgaac aacgtcgcgc tgagtatgat cagatgtggc aacatcgcaa
      421 cgatccgcaa tttagccgtc agttccagca tggcgacggt cagagtttta acgccgaaga
      481 ttttgacgat atcttctcgt caattttcgg tcagcatgcc cgccagagcc gtcaacgccc
      541 cgccgcacgc ggccacgata ttgaaatcga agtggcggta ttcctcgaag aaacgcttac
      601 tgagcataag cgtaccatca gctataacct gccggtttat aacgcctttg gcatgatcga
      661 acaggaaatc ccgaaaacgc tgaatgtgaa gatcccggca ggcgtcggca atggtcaacg
      721 catccgcctg aaaggccagg ggacgccggg cgaaaacggc ggtccaaatg gcgatttgtg
      781 gctggtgatt catattgcgc cacatccgct gtttgatatt gtcggccagg atctggaaat
      841 tgtggtgccg gttagcccgt gggaagcggc gctgggtgct aaagtcaccg ttccaacact
      901 gaaagaaagc attttgctga ctatcccgcc tggcagccag gccgggcaac gattgcgcgt
      961 taaaggcaaa ggtctggtga gcaaaaaaca gaccggcgat ctgtatgcgg tactgaaaat
     1021 cgtgatgccg ccgaaaccgg atgaaaacac tgccgcgctg tggcagcaac tggcagacgc
     1081 ccagtcgtct tttgatccac gtaaagattg ggggaaagca taatggctaa tgttacggtg
     1141 acttttacta ttaccgaatt ttgcctgcat accggcatct ctgaagagga attgaatgaa
     1201 attgtcggtt tgggggtggt tgaaccgcgt gagatccagg aaacaacctg ggtatttgac
     1261 gaccatgccg ccatcgtggt gcaacgcgcg gtgcgcctgc gtcatgaact ggctctggac
     1321 tggccgggga tcgcggtggc gctgacgtta atggatgata ttgcgcacct gaagcaggaa
     1381 aaccgcctgc tgcgccagcg gctttcccgg tttgtagctc atccgtgatg tgtgtttgcc
     1441 ggatgtgatg acgcatctta gcctgcctgc aaagagtact tttcctgtgg gccggataag
     1501 gcgtgttcac gccgcatccg gcggcggtgc cctaatgcct gatgcgacgc taaacgcgtc
     1561 ttatcaggct aacaaacgac gttatctgtt ttggtggtcg ccctcaacta acaccagcaa
     1621 taactggctt agtgccgcgt aaaaaccaaa gctgtcatac tgatggcaac gcgcaacaaa
     1681 ctctggtaac cattgccgca acgccgtgag tgttttttgc cgtaaactgt cgattcttcg
     1741 cgcaggaacc gtcccctctc ccagcgaaaa atgcagatga ctcaacaact cgagatagat
     1801 cgccagatga tctgtcggtt cgttgaaatt gccgctggtt tccatccctg cctcaactaa
     1861 caagcgttta atctcttgct cgtcctgttt gtaggccgat gcatacggca gcgccgcttg
     1921 tttgtcagtc atcagaaaca ggccgcaaaa gtcagcggcc agttccagac gggcatcgtc
     1981 acgtactgtc agagtggcaa cacggttttc cagctcatcc accgccgcag tgagcggcgg
     2041 ttcgcttttc agcaacgaaa accattcagc catctgcgca ctggcgattt gcgtcagttg
     2101 ttcatcgtcc agttcacggg agaacaactg cgccagccag gcgtaaacac aggctatctg
     2161 ttgtgctgtc agcgtggtca taatttcacc tgcgacgcgg gaacatattc gcactgcgcc
     2221 accatctcca cggggccgtt aaacgccgtt acctgctcca ctgttccgtt gcacttctca
     2281 atttccacca gcgtagtgtg cgcactggtc gcctgcgcca gctgcgaagt accgatgtca
     2341 atggtcaaca cgttggggtt accgtatttg cacagcgcac caggctcgcc gcctttatct
     2401 ggatcgtgcc atgccccttc gtgaattcgc gccacgccgg gtgcatagcg gtcagaaact
     2461 actgcccctg ccaacacctg accgcgagcg ttaaagacgc gtaccacatc accgttacga
     2521 ataccgcgcg cgctggcatc ctgcgggtta atgaacactg gctctttacc cgctaccgta
     2581 tattgctgac gcagagtttc cgactcacat aactgcgagt gaagtcggaa atccggatgc
     2641 acagattgca gatgcaacgg atacgtctgc gagccgggcc caccgtggga gcgttcgatt
     2701 ttctcaaacc acatcggatg cccctgacaa tcgtcgtagt tcatatcggc gatggttttc
     2761 gaatagatct caatcaggcc actcggcgtg cccagcggtt cgagatctgg atcttcgcgg
     2821 aatgcctggt ggcgaacaaa catctgcgga tggtcaaact cgacgtactc tttgttattc
     2881 cagaagtcat caaacgctgg caaatgaacg ccgcgtcctt tgccttgctg aacaccttcc
     2941 tgccagatgc gtttcagcca gcccatttcg tccagccctt cggtaaaggc ttcttcgcga
     3001 ttaaagcggc ggcacagctc gcggaaaata tcaaagtcgt tgcgcgcctc gaactgcggc
     3061 ggcacgacct gtttcatggc gataatgcca cggttggagt ggttgccgta ctggtcgaga
     3121 tcgttacgct caaactgcgt ggtcgcaggc agcacgatat cggcaaagcg gcaggttgag
     3181 gtccactggt tatctatggc gataaccgtt tccagtttgc gccagccttc aataatgcgg
     3241 ttgatctgct gatggcgatg gaaggggtta gttccggcaa aaatacacat tttcagcggc
     3301 ggcagtttta ccgatttacc gttccagttg attactttcc ccggttcgag gatcgcatcg
     3361 ataaaacggg caatcggaat ggtgctgctg tagcctttgt aatcactgtt gtcgtgaaca
     3421 ggcggaatcg acgtagagcc ggagaaacca ctcagaataa cgcctttacg ccccggcgtg
     3481 cctgcgccgt tatagtgcca gccaaaacca aagccaccac ctggcaggcc aatttgcccc
     3541 agcatcgccg ccagaaccac aatcatccac gcccactgtt caccgtgctg catacgctgt
     3601 acgcaccagc cagcaataat ctgcgttctg ttcgccgcca tctgccgcgc cagtccacga
     3661 atggtttcgg catcaatgcc ggtcagtttt tcagcccatg cggcatcttt cggctgaccg
     3721 tctttctcac ccagcagata cggcaggaac tgctcaaaac ccacacagta gttagcgagg
     3781 aagtttttgt cgtacaggtt ttcactgtac aacgtatgcg ccagcgccag ttgcagcggc
     3841 acgtcagttt gcgggttaac cgcaatgtgc ttcacatgct cgcgccccag atactcatgg
     3901 gtggatgtaa caaccggatc gatgctaatg acctcaattt caccggcggc gactttcgct
     3961 ttcaactgcg cgtaatattc ataaacatcg tgatccgggc accaccagtt cgcttgctgg
     4021 tttttcagca aatcggagcc ccacagcaca atggttttgc tgttctgcaa taccagcggc
     4081 caggaggttt gctgttcata cacttccatc gaaccgacta cgcgcggcag gatcacctgc
     4141 gcagcaccag tagagtaatc tccgcccgta ccaacgctat taccatgcaa ggcaatagct
     4201 ttcgccagca tccccgaagc gttatggaac atccccgtcg attgccaacc actggcggtc
     4261 agcaaggcac tcggcccgtg agttttctgt acgcgttcca gttcttcata gaacatgtcg
     4321 agggcttcat cccagctcac acgcacaaaa cggttatcac cgcgctggga ggtgtcgctc
     4381 agatggcgct tacgcagcca gtccacgcgt accatcggat aacgaatacg cgccgcgttg
     4441 tgtacatgat ccggcaatcc ggcaatcatt ttcgacggat atttatccag ttcgaacggt
     4501 tttgccgcca caaagcgacc atccttcacc gtcgcgcgga tagcccccca gtgcgacccg
     4561 gtaagaatgc cctctttcga gatgacagcc tcagtcgccg cttgcgccgc agtcgcacgg
     4621 cgcgacgtta acaatgacgg ccccagcatc ccggcgacgg ttaagccgcc gagttgtgcc
     4681 agaaaacgcc gacgtgatgc ctgaaagaga tcgttattgt tcattatttt tcttccttct
     4741 tatcgccgtg agccttacct gcggtgtcag acgcattcat ttgcagatat ttcaacaagg
     4801 tgcgttcttc acgtttatcg aggctggtaa agccgatcat gccgttgagc gtgccaatcc
     4861 agccgttagc gtcaaagtga gagatttccg gtgcgccgtg gcactggtta caggtgccgt
     4921 tgtacaacga atccgcataa gcccagatcg gtttgatatc gttcaccatg tcacctttct
     4981 tcatccacgc ggtggcctgc aacttgctcc actcggtatt ggtgtcggca acggtggttt
     5041 tctccagcgt ttttacctgc tgctgcacat caccacgaat cgaggcaaca aagatgcgtt
     5101 tacctgggaa ttgggtgagt acacgctgac gtccggcgct ttctgtccag ccggtaattt
     5161 caatttgcag ccagtcgccg tcacgtttaa ggactttcac ttccgaggca ggcagcagag
     5221 aaccagaggc ttctttatcg cctttcgccg cataaattgg cttaatatca atagagtaca
     5281 gcgtgtcacc actgtcatta gcaccggcgc gcagctcatc gaactgctta cggaagccgc
     5341 tactcatatc cggtaactgg tgggcaatac ctttatgaca gtcgatgcag gattgattat
     5401 ctttcgctgc caccttcatc tgacgtgccg cttcaggatg ctgcttcgca tgatccatcg
     5461 catcgtagtt atggcaggag cgacaggttg ccgagttgtt ttctttcatt cgcgcccatt
     5521 cacgctcggc aagttccgcg cgtttggctt caaatttttc aggtgtatca atggagtggg
     5581 caataaaggt ctggtagata tcgttgctcg cttccagttt gcgcttcacc atgcctggaa
     5641 tatccggcgg gatatgacag tcatggcatt cagctcgcac gccggaggcg ttctggaaat
     5701 gcaccgactg tttatattct tcatacactg gttgcatact gtggcaactg acacaaaatt
     5761 cggttgtgct ggtgactttg atcccaacgt gtggcaatac aatcagcgca atgccaatca
     5821 caatcccaat agcgaccagc gccagtaccg accaacgagc actgggtcgg cgtagcgcgt
     5881 tccagagttt ccgcataata gcccctgtaa aattatggtt tagtgaagcg atcttaatga
     5941 gtaaatatga acagcggcac tggtcaggat gaacggctta cggcagaata tgaacagata
     6001 tgaacagaat gagtaaaacc ctctgatgcc acatcacatt gttattgttg aagatgagcc
     6061 ggttacccag gcgcgattac aatcctactt cactcaggag gggtataccg tttccgttac
     6121 ggcgagcggt gccgggctac gggaaattat gcagaatcag ccggtggatt taattctgct
     6181 ggatatcaac ttgcccgatg aaaatggcct gatgttaacc cgcgccctgc gagaacgctc
     6241 aacggtgggg attattctgg ttaccggacg cagcgatcgg attgaccgta ttgttgggct
     6301 ggaaatgggc gcagacgatt acgtcaccaa accgctggaa ctgcgcgaac tggtagtacg
     6361 ggtaaaaaat ctgctctggc gaatcgacct cgcgcgacaa gctcaaccgt acactcagga
     6421 caactgctat cgctttgcgg gttattgcct gaatgtgtcg cgccatacgc tggagcggga
     6481 tggtgagccg attaaactga cccgcgcaga gtatgaaatg ttggtggcat ttgtgaccaa
     6541 tccgggcgaa attctcagcc gtgaacgtct gctgcgtatg ctctctgcgc gtcgggtgga
     6601 aaaccctgac ctgcgcaccg tcgatgtgtt aattcgtcgt ttacgccata aactcagcgc
     6661 ggatttactg gtgacgcaac atggcgaagg ttatttctta gccgctgatg tgtgctgata
     6721 aaaatagacc ggacgaaatc ccccaggcga cagcgagcgg cggatatgtt cgcggtcggc
     6781 atttttcggc gtcagaacta aaatcggtgg gctgacatta tcagacaccg attgcccctg
     6841 taattgcctg atggcctgct caactgccag ttccccctgc cagaccattt gatcgctggc
     6901 agccataatc actcttcccc gcttcagccc gcgatacacc tgatgtgaaa gataaaacga
     6961 caccacggta agcggcgttt tcaggttacg cccttccccc attgccgcct ctgccgcaat
     7021 ggccgttccg gcaacgacgt cgatttctgg atggcgttcc agcatctcct gcaacaggtt
     7081 acgctggatt tcaatatcgt tatcaccaag cgcaatatca acaatacgca ccgggcttcc
     7141 ggcaatggct gcgcgaaaac cctcgaccat ctccttactg cccccggcgt tatcgggtcc
     7201 gggcatcaac agcacattca gtggtttacc gtgcgcccat tgcaccagat atcgcccagg
     7261 ttgatagccc atctgaaacc agggcacacc aacgcggctt ttcacctggg gagcatcaat
     7321 agcatttacc agctcgatca ccggcagatt tgctacttgc ttttgcagat cgggaaatga
     7381 cgtcgtgcta ctaccgagca aaatggcctc tgcgccccac tgtttacact ggtcgatttg
     7441 tgcttgctgg gtagccaact ggctgtagcc gcctgcctcc agcactttta aatccacacc
     7501 gtagcggcga gcagcctcct gcataccata gttcaacgat aaccaatatg aatctttcag
     7561 gctgggataa agcgcgcaca gtttccatac gcgtttggct tttagcggca tagaggcttg
     7621 caccgtgaaa tgctgcgcat catgccagcg caacaggtta tcagccgaaa atgccggcaa
     7681 catgaaaagg gaaagaagta aaaatagcag cacgcgcatg atagcctcat caataataag
     7741 gctttatgct agatgcattc cgctttgcga ctcaacattt ttcaccttaa gtgcaccgac
     7801 cgtgaattta accctgaccc gaagactctg gatgggcttt gccctgatgg cgctgttaac
     7861 cctgaccagt accctggtgg gatggtacaa cctgcgcttt atcagccagg tggaaaaaga
     7921 caatactcag gcattgattc ctaccatgaa tatggcgcgc cagttgagcg aagccagcgc
     7981 ctgggaactt ttcgccgcgc agaacctgac cagtgccgat aacgaaaaga tgtggcaggc
     8041 gcaggggcga atgctcaccg cacaaagcct gaagattaat gcgttgctgc aagcgttacg
     8101 ggaacaaggt tttgatacca ccgctattga acaacaggag caggagatct cccgttcgtt
     8161 acgtcagcaa ggggaactgg tggggcggcg tctgcaactg cgccagcaac aacagcgact
     8221 cagtcagcag atagtcgctg ccgccgatga gatcgcacgc ctggcgcaag gtcaggcgaa
     8281 taatgcggca acctccgccg gagcgaccca ggccgggatt tacgatttga tcgaacaaga
     8341 tcagcgtcag gctgctgaaa gtgcactcga tcggctgatt gatatcgatc ttgagtatgt
     8401 taaccagatg aatgaactgc gccttagcgc tctgcgggtg cagcaaatgg tgatgaatct
     8461 ggggctggag cagatccaga aaaatgcgcc aacgctggaa aagcagctca ataatgcggt
     8521 gaaaattctg caacgtcggc aaatacgcat tgaagatccg ggagttcgtg ctcaggtcgc
     8581 aacaacgtta actaccgtta gccaatatag cgatttgctg gcgctgttcc agcaggacag
     8641 tgaaatcagc aatcacctac aaactctcgc acaaaataac atcgcccagt tcgcgcagtt
     8701 tagcagcgaa gtcagtcagc tggtcgacac cattgagctg cgtaatcagc acggactggc
     8761 gcatctggaa aaagccagtg cacgcgggca atacagcctg ttattgctgg ggatggtttc
     8821 actttgcgca ctgattctga tcctctggcg cgtggtttat cgctcagtca cgcgtccact
     8881 tgccgaacaa acgcaggcgc tgcaacggct gctggacggt gatattgact cccctttccc
     8941 ggaaaccgct ggcgtacggg agctggacac catcgggcgg ctgatggatg cgtttcgcag
     9001 cagtgttcat gcactgaatc gccaccgcga acagctggcg gcgcaggtca aagcgcgtac
     9061 agctgaattg caggaactgg tgatagaaca ccgacaagca cgggcggaag cagaaaaagc
     9121 cagccaggca aaatcggcgt ttctggcggc gatgagccat gagatccgca caccgctgta
     9181 cggtattctc ggcaccgcac agctactggc agataacccc gcacttaacg cccagcgtga
     9241 tgatttgcgg gcaattaccg actccggcga atcgttgctg accatcctca acgatattct
     9301 cgattattcc gctatcgaag caggtggcaa gaatgtttcg gtcagcgatg aaccctttga
     9361 accgcgaccg ctgctggaaa gtaccctgca attaatgagt ggacgggtga aaggtcgccc
     9421 gattcgcctg gcaacggcaa ttgccgacga tgtaccgact gcgttaatgg gcgatccgcg
     9481 gcgtattcgt caggtcatta ccaacctgtt gagcaacgcc ctgcgtttta ctgacgaagg
     9541 gcagatcgtt ttacgtagcc gtactgatgg tgagcaatgg ctggtcgaag tggaagacag
     9601 cggctgcggt attgatcccg cgaaactggc agaaatcttc cagccattta ttcaggtaag
     9661 tggcaaacgc ggcggcaccg ggctggggct gactatcagt agccgtctgg cccaggcgat
     9721 gggcggcgaa ctgagcgcca ccagcacgcc ggaggttgga agctgttttt gtttacgctt
     9781 gccgttacgt gttgccacgg cacccgtgcc aaaaacagtc aatcaggcgg tgcgtcttga
     9841 cggtttacgt ttgctgttaa ttgaagataa cccgctaacc cagcgaatta ccgttgagat
     9901 gctgaacacc agtggtgcgc aggttgttgc tattggcaat gccgcgcagg ctttagagac
     9961 actgcaaaat agcgaaccgt ttgctgccgc actggtggat tttgatctgc cggatgtcaa
    10021 cggcattacg cttgcccgac aactggcacg acaatatccg tcgctggttt tgattggctt
    10081 tagcgcccat gtcattgacg aaacgctgcg ccagcgtacc agttcactat ttcgcgggat
    10141 tatccctaaa ccggtgccgc gtgaagtgct cggtcagtta ctggcgcact atctccaact
    10201 gcaagccaat aacgatctac cactggatgt atcgcaactc aatgaagacg ctcagttaat
    10261 ggggacggag aagatccacg aatggctggc attatttaca caacatgccc tgccgcttct
    10321 cgatgaaatc gacattgccc gcgccactca ggatagcgag aaaataaagc gtgccgcgca
    10381 tcagctaaaa agcagttgct cgagtctggg aatgcgtagc gccagccagc tctgcgcaca
    10441 actggagcag cagccattat ctgcccccct tccacacgaa gaaattacac gcagtgttgc
    10501 cgctctggaa gcatggttga ataagaaaga cctgaacgcg atttgaatca atatcagcca
    10561 gttaagaata actagaatag tggcatctgg tatgcaactt aacggactgg caaaatcatg
    10621 gcagagaaaa aaagaacccg ctggcagcgg cggccaggca cgacgggcgg caaattaccg
    10681 tggaatgact ggcacaatgc cacgacctgg cgtaaagcga cgcaattatt gctgttggca
    10741 atgaatattt atattgccat cacgttctgg tattgggtgc gctattacga aacggcaggt
    10801 agtacgatat ttgtcgccag accgggaggt atcgaaggct ggctaccgat tgccggtctg
    10861 atgaatctga aatatagcct tgcaacaggt cagttaccgt ccgtccacgc cgccgcgatg
    10921 ctgttattgg tcgcttttat cgtcatcagt ctattactca aaaaggcctt ttgctcatgg
    10981 ttatgcccgg ttggtacgct ttctgaatta atcggcgctc tcggtaacaa actgtttggt
    11041 cggcaatgtg tccttccccg ctggctggat attcctctgc gcggtgtaaa gtatttgctg
    11101 ttgagttttt ttctctatat cgcgttattg atgcctgctc aggcgattca ctattttatg
    11161 ttgtcgccct acagcgtggt gatggacgtt aaaatgctcg atttctttcg tcatatgggg
    11221 accgcgacat taatcagcgt gaccgttttg ctgattgcca gcctgtttat tcgccatgcc
    11281 tggtgtcgtt atctttgccc atatggcgcg ctgatgggcg tggtttcgct attatcgccg
    11341 tttaagattc gtcgcaatgc cgaaagttgt atcgattgtg gcaaatgcgc aaaaaattgc
    11401 ccatcacgga tcccggtcga taaattaatt caggtacgaa cagtggaatg taccggctgt
    11461 atgacttgcg tagagtcatg tccggtagcc tcaacattga ccttttcact gcaaaaacct
    11521 gcggcaaata aaaaagcctt tgcgttgtct ggctggttaa tgacgctact ggttctgggg
    11581 attatgttta cggtgattgg ttacgcaatg tatgcaggag tatggcaaag cccggtgccg
    11641 gaggaattgt accgacgctt aattccgcaa gcgccaatga ttggtcacta aattagcaac
    11701 aagcggaaca tcacctcgca cggggtgatg ttcacaaaat cacattattt tgattttgac
    11761 atcataactt tcaagcccgg tcattttttc tcgcgcggtg aagcgaattt cagagacttc
    11821 tttccctgta ttcttgttca gctcggcgat tttttgcgcg ataaagtcgg cgatttccgt
    11881 ttcggcttgt tttttaactc ttcaatattc actgttaacc tcttctggtc ttaaaagaca
    11941 gcaacacgcc agtaaggtgt tggttcccgg taccctggtc aggtaagaaa tgttataagc
    12001 catctccccg aaagattcaa taatcaacag gttatcgctt actatacata cagcgttgaa
    12061 aaaaaataag acagatataa aaaaggctcc acaagggagc ctgatattat tttgtgaaat
    12121 ccagcggtgc atggtcagtg cgtatttgca gcgcgacgtt atcaaaggtg atcatcgtcg
    12181 atttacaaaa aatacactta gcgcccaaag ggtttcgctc cgtcacatcg aatgccgatg
    12241 tgcggtactg tgaaccatga caacatggac agcgaaaatg gagatgttga gtaataacgg
    12301 caaccttaga gcgtaactac gttcgctgcc gcggggccac gttgaccctg ctcaatagaa
    12361 aattcaactt tctgattttc gttcagcgtg cggaattcat tgctctggat ggcggtgaaa
    12421 tggacgaaaa cgtctttgct gccatcatca ggagtgataa agccaaaacc tttatctgcg
    12481 ttaaaccatt ttactaaacc agtcatttta ttagacatat tttacttcct tcattttggg
    12541 cctttcggca aacagggctt taatacagaa actacttagt gttcaggaga gagactcaaa
    12601 agaaggggat aatgcctgat aatgagaact gctttagtaa actactttgt atgctgtctg
    12661 tctttccaac cgacgcagct attaacgcat gataattgag attaagcaaa ttttatttta
    12721 gccgtccggc ggtcttaaat atcgctggct ttattttatt aaccccgtat agtgcagcgc
    12781 agtattttca gtaaggaatt tgtttgtccc gtaaaatgac aggaattgtc aaaacctttg
    12841 atcgcaaaag cggcaaagga ttcattatcc cctccgacgg tcgtaaagaa gttcaggtcc
    12901 atatttccgc attcactccc cgcgacgcag aagtgcttat accaggatta cgcgtggaat
    12961 tttgccgcgt aaatggcctg cgaggaccaa cagcggcaaa tgtttatctc tcttaatatg
    13021 acgctgcttg tttaaagctg actggtttta ttggcatagt tcaatagtca acaccatgta
    13081 ttataacaca agttactttt tcaccggaaa actaatccat ttaaccgaat atctctacaa
    13141 ctaatagtta taaatgccac ttaacagcga gttatagtac cacttgaaac tatattgcca
    13201 acaatagtta atagctttct taacaatttt cttatcttta atttgtattt tgctaagatt
    13261 tatcttatgc tcacaggtcg tatttccaga ggggaatagt taacaatctt atatttccct
    13321 ctgcgcgcct ttctcacccg ctgtatatag tgcaagtggt atgaggaaag ggagatttca
    13381 caaggatgtt atactcaaag tagacaatcc tgatctcaag cgtacgtatt gtccatgcga
    13441 tgaataaagg aaaagttatg aaacacaagc tttctgcaat ccttatggcc ttcatgttga
    13501 cgacgccagc tgcgtttgct gcccctgaag cagcaaacgg gaccgaggca acgacgggta
    13561 ctactggcac cacaacgacc actaccggtg caaccacgac ggctgctacc actggtggcg
    13621 tggctgctgg cgctgtcggc acggcgacag ttgtcggtgt ggcaacggct gttggtgtgg
    13681 cgacgttggc agtagttgca gcaaatgaca gtggcgatgg cggttctcat aacacctcaa
    13741 ccaccacgtc cactacgcgc taattctgct taaacgcgtc gaaattaaag ttaaataata
    13801 cccggttgcg atattcgtac cgggctttag agatttgcat ttttttaatt aacctttcaa
    13861 ggattaaagg tgcgccctct tattttatcg attttcgcac tatttcttgc gggatgtacg
    13921 cacagccagc aaagtatggt cgatacattt cgcgccagcc ttttcgataa tcaggatatc
    13981 accgtagcgg atcagcagat ccaggcgttg ccttattcca cgatgtattt acgccttaat
    14041 gaagggcaac gaatctttgt ggtactggga tatatagaac aagaacaaag caaatggtta
    14101 tcccaggata acgccatgct ggttacccac aatggacgtc ttttaaagac cgtcaaactt
    14161 aataataatc tgctggaagt gacaaattcc gggcaggacc ctctgcgtaa cgcgctggca
    14221 ataaaagacg gcagccgctg gacgcgcgat attctctgga gtgaagacaa ccattttcgc
    14281 tctgcgaccc tgagttccac tttttccttt gctggattag agacgctgca tattgcgggt
    14341 cgcgatgtgc tgtgtaatgt ctggcaggaa gaggtgactt ccacgctacc agaaaaacag
    14401 tggcaaaaca cattctgggt cgattcggct actggccagg ttcgtcaaag tcggcaaatg
    14461 ttaggcgcag gggttattcc cgtagaaatg acgtttctta aacccgcacc atgaataaat
    14521 tacagtcgta tttcattgcc agcgtacttt acgtaatgac accccatgcc tttgcgcaag
    14581 gaacggtgac tatttatctg cctggcgaac aacagacact ttccgttggc cccgtggaga
    14641 atgttgccca gttagtgaca caaccgcaac tgcgcgatcg cctctggtgg cctggtgccc
    14701 tgctgaccga ctccgcggtg aaagctaaag cgcttaaaga ctaccaacat gtcatggcac
    14761 agcttgcttc ctgggaagca gaagctgacg acgatgtggc ggcaacgatt aaatccgtgc
    14821 gccagcagtt actcaatctc aatattactg gtcgcttacc ggtcaaactg gaccctgatt
    14881 ttgtacgagt ggatgagaac agcaatcccc cgctggtcgg gaactatacg ctctataccg
    14941 tacagcgccc ggtgactatt actcttctgg gtgcggtttc tggggctggg caattgccct
    15001 ggcaggcagg ccgtagcgtt accgattatc tgcaagatca cccccgtctt gcaggcgcgg
    15061 acaagaataa cgttatcgtg attacgcctg agggtgagac tgtcgtcgcc cctgttgccc
    15121 tgtggaataa aagacatgtg gaaccaccgc ctggcagcca gctttggtta ggcttttctg
    15181 cgcatgtact gcccgaaaaa tatgctgatt taaatgacca gattgtgtct gttttaacgc
    15241 aacgtgttcc tgactgatga agaagaattc ttatctttta agctgcctgg ccattgccgt
    15301 ctccagtgcc tgtcatgctg aagtattaac ctacccggat ccgctgggtt cgtcgcaatc
    15361 ggactttggc ggcacaggat tgttgcagat gccaaatgcg cgcatcgcac cggaaggtga
    15421 attcagcgtc aactaccggg ataacgatca ataccgtttc tactccacct ccgtggcgct
    15481 gttcccatgg ctggaaggca ccattcgtta tacggatgtg cgcacacgta aatatagcca
    15541 gtgggaagat ttcagcggcg atcagtcata caaagacaaa tcattcgatt ttaaacttcg
    15601 cctgtgggaa gaaggttact ggctaccgca agtggcgttt ggtaaacgtg atattgctgg
    15661 tacgggtctg tttgacggtg agtatctggt ggccagcaag caagcggggc catttgattt
    15721 caccctcggg atggcatggg gctacgccgg taatgcgggc aatattacca acccgttttg
    15781 ccgggtgagc gataaatatt gtcatcgcgc agagtctcac gatgcgggcg atatcagctt
    15841 tagcgatatc tttcgtggcc cggcttccat ctttggcggc attgagtatc aaacgccgtg
    15901 gaatcccctg cgtctgaaac tcgaatacga cggcaacaat taccagaatg atttcgctgg
    15961 caaactgcct caggcaagcc atttcaacgt cggtgcagtt tatcgcgctg ccagctgggc
    16021 agacctcaac ctgagttatg aacgcggtaa cacgttgatg tttggtttca ctttacggac
    16081 caatttcaac gatctgcgcc ctgccctgcg cgatacgcca aaaccggcat atcaacctgc
    16141 gcctgaatct gaaggattgc agtacaccac ggtagcaaac caacttaccg ccctgaagta
    16201 taacgcgggc tttgacgcgc cagaaattca gctacgcgat aagacgctgt atatgtctgg
    16261 tcagcaatac aaataccgtg actctcgtga agcggtcgat cgtgccaacc ggattctggt
    16321 gaataacctg ccgcaaggcg ttgagaagat tagcgtgacg caaaagcgcg agcatatggc
    16381 gatggtgact accgaaaccg acgtagccag cctgcgcaaa cagctggcag gtacagcgcc
    16441 tggtcaatca gagccactgc aacaacaacg tgttgaagct gaagatcttt ctgcctttgg
    16501 tcggggctac cgtattcgtg aagatcgctt tagctactct ttcaacccaa cactttcaca
    16561 gtcgctgggc ggcccggaag atttctatat gttccagctg gggctgatgt ccagcgcccg
    16621 ctactggttt accgaccacc tgctgcttga tggcggtatt ttcaccaata tttacaacaa
    16681 ctacgacaag tttaagtctt cgctgttgcc cgcggactct accctgcccc gcgtgcgcac
    16741 gcatatccgt gattacgttc gcaatgacgt ttatctcaac aacttgcaag cgaattactt
    16801 tgccgactta ggcaatggtt tctatggcca ggtgtatggc ggttatctgg aaacgatgta
    16861 tgccggtgtc ggttccgagc tgctttatcg cccgctagat gccagctggg cgctgggtgt
    16921 ggacgttaac tacgtgaagc agcgtgactg ggacaacatg atgcgcttca ccgattattc
    16981 cacgccaact ggtttcgtga cggcttactg gaacccgccg acgctcaatg gcgtactgat
    17041 gaaacttagc gttgggcaat atctggcaaa agataaaggg gcaacgatcg acgtcgccaa
    17101 acgctttgac agcggcgtgg cggtaggggt atgggcggca atcagtaacg tatctaaaga
    17161 tgactacggc gaaggcggct ttagtaaagg tttttatatc tcgattccgt tcgacttgat
    17221 gaccattgga cctaaccgca accgcgcggt ggtttcgtgg acaccattga cgcgtgatgg
    17281 tggacaaatg ctgtcacgca aataccagct ctatccaatg acggcagagc gagaagtacc
    17341 ggttggacaa taacctgacg taattcataa cattacattg actgataaat ataaaccaat
    17401 gaaaaagaac atttttaagt tttcggtact gacattggct gttctgtctt tgacagcatg
    17461 tacccttgtt cctggtcaga atttatcgac cagtaacaag gatgtcattg aactacctga
    17521 caatcagtat gacctggaca aaatggtcaa tatctatccc gtcacgccag gattgattga
    17581 tcagttgcgc gccaaaccga ttatgtcgca ggctaacccg gagctggagc agcaaatcgc
    17641 gaactatgaa tatcgtatcg gcattggtga tgtgttgatg gttacggtct gggatcaccc
    17701 ggaactaacc acccccgcag ggcaataccg tagcgccagt gataccggca actgggttaa
    17761 cgccgatggt gcgattttct acccctatat tggtcgcctg aaagtggccg ggaaaacact
    17821 tacccaggta cgcaacgaga ttacagcgcg tctggattca gtcattgaaa gcccgcaagt
    17881 cgatgtcagc gtcgcggcgt tccgctcgca aaaagcgtac gtcaccggtg aagtcagcaa
    17941 atcgggtcag cagccgatta ccaatattcc tctgaccatc atggatgcga ttaacgccgc
    18001 gggcggtctg accgcagatg cagactggcg caatgtggtg ctcacccaga atggcgtaaa
    18061 gaccaaagtg aacctctatg cgctgatgca gcgtggcgac ttgcgccaga acaaactgct
    18121 gcacccggga gatattttat ttattccgcg caacgatgat ctgaaagtct tcgtgatggg
    18181 tgaagtgggt aaacagagca cgctgaaaat ggaccgcagc ggtatgaccc tggcggaagc
    18241 gctgggtaac gctgagggta tgaatcagga tgtcgctgat gcgacgggga tttttgttat
    18301 tcgtgcaacg caaaacaagc agaatggcaa aatcgccaat atttatcagc tgaatgcgaa
    18361 ggacgcatcg gcaatgatac tcggaacgga attccagctt gaaccttacg acattgttta
    18421 tgtcactacc gcgcctctgg ctcgttggaa ccgtgtgatt tccctgctgg taccgacgat
    18481 ttccggggtg catgacctga cagagacttc acggtggatt caaacatggc ccaactaaaa
    18541 tttaactcaa tcctggtggt ttgtaccggc aacatttgcc gttcgcccat tggcgagcgc
    18601 ttgctgcgta aacgtctgcc gggcgtaaaa gtcaaatcgg caggtgttca tggtctggta
    18661 aaacaccctg cagatgcgac agcggcagat gttgccgcta atcatggcgt atctcttgaa
    18721 ggacatgccg gacgtaagct cactgctgag atggcacgaa attacgatct gattctggca
    18781 atggagtcgg aacatattgc tcaggttacc gcaatcgctc ccgaggtccg cgggaagaca
    18841 atgctgtttg ggcaatggct ggaacagaaa gagatcccgg atccctatcg taaaagtcag
    18901 gatgcatttg aacatgtcta cggaatgttg gagcgcgcca gtcaggaatg ggcgaagcgc
    18961 ctcagccggt aatttgagtt tataaaatac gatgacaact aaaaatatga atacgccacc
    19021 aggcagcact caggaaaatg agatcgatct gcttcgtctg gtcggcgagt tatgggatca
    19081 ccgtaagttt attatcagcg tgaccgcgtt attcacgctg atcgctgtcg cttactcgct
    19141 gttaagcaca ccaatttatc aggcagatac tctggtccag gttgagcaaa aacagggcaa
    19201 cgccattctc agcggcctga gtgatatgat ccctaactca tcgcccgagt ctgcaccgga
    19261 gatccaactg ctgcaatcgc gcatgattct cggtaaaacc attgctgaac tgaatctgcg
    19321 cgacatggtt gagcagaagt attttccgat tgtgggtcgc ggctgggcga gattaaccaa
    19381 agagaaacca ggtgagctgg cgatcagctg gatgcatatt ccacaactga atggtcagga
    19441 tcagcaactg acactcacgg ttggggaaaa cggccactat acgctggaag gtgaagagtt
    19501 caccgtcaat ggtatggtcg gccagcgtct ggaaaaagat ggcgttgcgc tgactatcgc
    19561 ggatattaag gccaaaccag gaacacagtt tgtcctgagc caacgtaccg aactggaagc
    19621 gattaacgcg ttgcaggaaa cctttaccgt tagcgaacgc agtaaagaaa gcgggatgct
    19681 ggaacttacc atgactggtg atgatcccca gttgattact cgtattctga acagcatcgc
    19741 taacaactat ttgcaacaga atatcgctcg ccaggcggcg caggattcac aaagccttga
    19801 attcttacag cgccagttgc cagaagtgcg cagcgagctg gaccaggcgg aagaaaaact
    19861 caacgtttat cgccagcagc gcgattcggt tgaccttaac ctggaagcca aagccgttct
    19921 tgagcagatt gtgaacgttg ataatcaact caatgagctg accttccgcg aggcagagat
    19981 ctcccagctg tataagaaag atcacccaac ttatcgtgcg ctgctggaaa aacgccagac
    20041 gctggagcaa gaacgcaaac gcctgaataa gcgggtatcg gctatgcctt ccacccaaca
    20101 ggaagtgttg cgtttaagtc gtgacgtaga agcgggccgt gcggtatatc tgcaattact
    20161 taaccgccag caggagttga gtatttcgaa atccagtgcc attggtaacg tgcggattat
    20221 cgacccggca gtcactcagc cgcagccagt gaaaccgaaa aaagcgttga atgtggtgct
    20281 tggttttatt cttggcctgt ttatttcggt gggtgccgtg ctggcgcgtg cgatgttgcg
    20341 tcgtggtgta gaagccccgg aacaactgga agagcacggc atcagcgttt atgccaccat
    20401 cccgatgtcc gagtggctgg ataaacgtac ccgtctgcgt aagaaaaatt tattttctaa
    20461 tcagcagcgc catcgtacta aaaatatccc cttcctggcg gtggataacc cggcggattc
    20521 tgctgtggaa gccgtacgtg cgctacgaac cagtctgcat ttcgctatga tggagactga
    20581 gaataacatt ctgatgatca ccggtgcgac gccagacagt ggtaaaacgt ttgtcagttc
    20641 aactctggca gcggtgatcg cccagtccga tcaaaaagtg ttatttattg atgccgactt
    20701 acgccgtggt tattcgcata acctgtttac cgtgagtaat gaacatggct tgtcggaata
    20761 tctggcaggt aaagatgagc tcaacaaagt gatccagcat tttggcaaag gaggctttga
    20821 tgtgattact cgcggtcagg tgccacctaa cccatctgaa ctgctgatgc gcgatcggat
    20881 gcgtcaatta ctggaatggg cgaacgacca ttacgatctg gtgattgttg atacgccgcc
    20941 gatgctggcg gtgagcgatg ctgcggtcgt ggggcgctct gtcggcacca gcctgctggt
    21001 tgcgcgtttt ggcttgaaca ccgccaaaga ggtgagcttg tcaatgcagc gtctggaaca
    21061 ggctggcgtt aatattaaag gcgcaatcct caatggtgtg attaaacgcg ccagcaccgc
    21121 ttacagttac ggctataact attacggtta tagttactcc gagaaagagt aaaactgaag
    21181 gtaaagtgac cggatgcggc gtgaacgcct tttccggtct acgcattaga cagttcttcg
    21241 tttgtcatca gcctcagagc attcaggtag ctgaatgctc ttttttatac attacaaact
    21301 gcacgccggt atgcgtgctt cattcacgat ttgcgtaaaa ccggccaacg aacacatgcc
    21361 ctgcgcattt cgctcttcac atcctgccag ggtcagtttc acctctccgg gcggcgtatt
    21421 taatgacagc ggagttttat cacgcatctg ctgtaaagtc tggaagacca gcgaaacctg
    21481 aatccactgg ctgttatcgc ttagccgacg ccagcgttca aacaccagtt caccacctgg
    21541 cggcgtgtta tccggctgac ctggaagcgt ccagttgagc tccagtgcgc cgccgagatt
    21601 tgccagatta gtatcgtgtc cggcgataaa cagcactgaa gtgggtaatg tcacaccata
    21661 cgcctgtttt tgcggtggat ggggagtcaa cgctatcatg atcaaatcca acaacggggt
    21721 ggcgcggctg cgggcaacct ctggcgtgcg ttgtagtaaa taaaattgcg cgttatgcaa
    21781 acttagcaac gtgttccact ggtgtgaatc agtgatcctt ccccaccccg gctccggcat
    21841 tccctgtgcc tgttgcagga gaaatatttc cgtcagcatt gatgcgaggc ttaccgcacc
    21901 ggttaatgag acattgtcgg cgctcacctt gagttccgat ggtaatgcct gcgttaatga
    21961 acagctttcg tcctgtttct cacggttaag gcacaagttt gattgcggaa aattaagcac
    22021 ccgttccagt tcgcgaaacg ccgtttgccg atgcccggta aagtcagcaa ttgaccctcc
    22081 tgccctgctg aggatcgcgt cagtcacgtt cgcgttatcc agttggcaaa cgccagtttt
    22141 tagaggatta aataacggat cgggactgga cgtatctgcc tgggtatgta cggttattgc
    22201 acagtcaggt gccagcccgg cggcgaaggc ttcgcctgtt ttacgggtac gctcgtcgac
    22261 atcagcaata atcgcgacct gaccaggctg cgggcagccc tttttcgtca gcaatccgtc
    22321 ggctaccaga cgctggcgtt ggtaatgtcc gagataagcg attagctcac caccgcgcgg
    22381 tgtcagccaa cccagtttta ccggccagtt tggccatgcg tctggggtga catcctgcat
    22441 cagttgcgtg gccttggttg gggcacgcac accatgacga ctgacaatca ccacactttc
    22501 cagcttcagc tccggctccg gctcactctg agcgaatgca gattgcgggg ttaacggaat
    22561 cagaagagat aaaaatggga tcaagatcgc tttcatcgat atgtttccgc ttttcatatc
    22621 cgacattatt gattgcctga tacgacgcta atgcgccgca tcagacttac gttcgttaac
    22681 ttttcaatcg cgggtccagc cataccaaca caagggtgga gagcgaacac atcaacaaaa
    22741 tgccgacgaa ccaaagtaaa taccacattg ttcctgctcc ttagtacagc tcgttttcgt
    22801 tacggcggag agtttctgtc gtcatgcgcc cccacatttt gtagtagctc cagagagtgt
    22861 agagcaacac aatgggcaaa aatatcagca cgattaccaa cataatactc agcgtcagct
    22921 gactggaagt gctgtcccac aacgtcaggc tggagattgg actcacgctt gacggcatga
    22981 caaaggggaa cagcgtgatg cctgccgtga aaatcacgcc aaattgcatc aatgacgcca
    23041 tcaaaaatcc ccaacccggg cggccacgat aaatcgccat caccgtcagc aatgggcaga
    23101 agaaccccag cagcgggaag atccacaaaa cgggcgactc gacaaaatta ttcatccagg
    23161 caccaggtag cactgccacc agtttcatta acggattgga aggaccgtta gcatcctggg
    23221 cgagcagtac aaagccatca ataccgaccc acagccagta acccgccagc aaaaagcaca
    23281 gcatcaccaa cagtgcagcg cgtttagtcg acagctgtga acgcagatga atcacaccaa
    23341 cagttttcag ttgtaaccag acgccacctt gcaaaatcac catcccaagg ctgagcaatc
    23401 cgcacaataa agggaatggc gtcagcagtt gccagaagct gccgagatac tccacgcgta
    23461 attgcggagt gaaggcaaac ggcacgccga gcaacaagtt gccgaaggcg ataccgaaga
    23521 ctaccggcgg caccagactg ccgatgacca gaccggcatc ccacatttta cgccagcgtg
    23581 catcggcgat ttttccgcga taatcaaagg ccagcgggcg gaagaacagc gagcacagca
    23641 ccaggatcat cgccacataa aagccggaaa acgccgctgc atacactctg ggccaggcgg
    23701 caaataatgc cccacctgcg aggatcaacc agacctgatt gccttcccag tgcgcaccaa
    23761 cgctattaat cactatccgg cgttcatcat catcacgcgc caccagcggc agcaaacagc
    23821 caatccccat gtcaaatccg tcggagatca taaagaccac caggatcacg ccaatcagca
    23881 gccaccagat gaagcgcaat gtttcataat caaacatgat tttctccttt acccctgttg
    23941 ctgcgtcggt tgttcactct gcatcgcgct cggccccaga cgggcatatt tctgcatcag
    24001 gtagacttcg gcgattaaga acagggtgta aagccctacg atcatgatca gtgagaaagc
    24061 cagttgtcct gtggttaaag cggagtgcgc ggagtatgtc ggtaagatgt cctgtatcgc
    24121 ccacggctga cgaccaaact cggtcataaa ccacccggct tcaatcgcaa tccacggcaa
    24181 cggcaaactc cagagcgcca ttttcagcac ccagcgatgc tggtcgattt tgccacgcag
    24241 cgtctggaca agcgcaatca gcatcaccag tagcagcagg gaaccacagc ccaccatgat
    24301 gcggaaactc cagaataccg gcgcaacctg aggtatcgcg ccacgcatcg ccgcctggta
    24361 ctgtgcggct gtgacatgat tcatatccgg cgcatagcgg gagagcaaca tgccgtagcc
    24421 caggtcgcct tccagctccc ggaatgcctg caacacatgc ggctcacgat tgccttgcga
    24481 tatttcctgc attaacagcc aggccatacg tccgcgttgc aagcgtgggt aggtttcagc
    24541 catcaaattc ttcagacccg gcacgggttt atctaatgag tgagtggcga ggatccctaa
    24601 cagcacggga attttgatgg caaaggcgtt acgctcttga tcctgttccg gccaggcaac
    24661 cacatggaac ggtgcaggag caggttccgt ttgccactcc ccttccatcg ccgccagttt
    24721 taccggttgt acttgcgcga cttcatacgc agaactgtct ccgagttgca gggtaccgat
    24781 aatcgccaga gtaccgaaga cggaaccgat ggcaaacgag cgtaatgcga catcgcgctc
    24841 ccgtccgcgc agtaaatacc aggcgctgat cgccataata aacatggccc cggtcacgta
    24901 gcccgccatt acggtgtgca caaatttcac ctggctgacc gggttaaaga ccagctcgct
    24961 gaagctggtc atctccatac gcagggtgtc gatatcaaaa tgcgcaccgg tcgggtattg
    25021 catccaaccg ttggcattca atatccacaa tgcagagaga tttgaaccga acgccaccag
    25081 ccacgtcacc agcaggtgct ggtatttatt cagccgctgc cagccgaaga agaaaagccc
    25141 gacaaaggtg gattcgagga agaaggccat taatgcttcc atcgccagcg gtgcgccaaa
    25201 aatatcgccc acatagttgg aatagaatga ccagttagta ccaaactgaa actccatggt
    25261 caggccggta gccacgccaa gagcaaagtt gataccgaag agcttacccc agaagcgcgt
    25321 catatcgcgg tagattgttt tgccggtgac cacgtaaatg gtttccatga tagccagcaa
    25381 aaaaatcagc cccagggtaa ggggtacaaa taaaaagtga tacagcgcgg tcagagcaaa
    25441 ctgccagcgc gataaatcaa tgacatccca catgcgcact cctgtaggcg aagagagaaa
    25501 aataacccgt taatggtaga tctccctctt tatcctgaaa ctctacgacc agaggtattc
    25561 gccgattgcg atattcataa gcctgaaagc gatacttacc gtctttttac gtcggtgcag
    25621 cttccgccag ccactgacat acctcactaa gccgctgcgc agagtcgacc aaatcttctg
    25681 gcgctgccag caccacttcc ggtattgggc agatttcata actttccagt aacgggcctt
    25741 ttaaggtgtc cgtacagcgt aaatgccaga catggcgtaa ccccgtggcg ttgatatagc
    25801 tctcgccata gccaatggta cgaatctgaa tatttcccgg cccacagaga cgtgagagaa
    25861 aaagccgatc agcctcgctg atgggtaatt gcgtcagatt aatgctgtgc ggctgcgcgt
    25921 cggggttacg tacatgcgcc agtaactcat gcgccaacgg taggccattc atcaggccat
    25981 caatgggcgg cggtaacagc gaatctgtcg gcaagagatt ttgcgttgcc gcctgccaca
    26041 acgccagcgg cgcgcagcca gcctccagtt tgtcctccag caacttttcg ccgcgacgtc
    26101 tgcgcacccg ccataatccg cagaagatcg cctcctgtat ttcactttcg ctgtcgtcag
    26161 cctgctgaat gcgcactgac acttcacctt ccccgagaag cgtattgata aagtgacgat
    26221 cgtcggcgtt gagatttaac agatcgactg ccagcacctc gcccaacggc ggctggcgtt
    26281 gacttagttg atgttgtaac tcgtttaaca gcgcaatcac ctgcgggctg tgagcacatt
    26341 gctccagggc cgccatactc ggttcatcat tcacctgaca ggtgatcggc agtggattca
    26401 tgctgaaact gtcatcgttc ggttgcgttc ttggccccag cagatggaaa aaagtttcgc
    26461 tcatgaggca cgctcctgct gtggttcgac aagcccacgc atcaggttta tcagttccgc
    26521 ccacgggtga ataccattca gcacgccgcg atagtttccg ccggtaaaca ctaatgtggc
    26581 gggaaagcga aagacgccaa agcgatctcc gatggcttcg ctctgttcaa ggtcagcaat
    26641 cgccacctgc catgtgtagt cgggaaactc gcgcagtaat tcgccaatca ttaccggatt
    26701 atcgctgacc tctggcgtgc gtttcgggtc actgcttaat aacaccacgc cgtctggcgc
    26761 ttgcgtaagc cagtcgtcaa gacggaattc actaaccggc gtccagccgc gcgccagcat
    26821 tcgttgccac aacgcatcaa atggcgtgtc gttgctcatc cctgctcctc cagtgtcatc
    26881 ctgtactggc gcaagcgaac gccttcgtaa ttttccatcg acagacagtc ataattgaga
    26941 cagcgcgatt cattagccgg ttgcggcaca attccccatg cagccagctg cgccagcgcc
    27001 gcctgttccg ctgcgggcag ttgctcccgt gccagttcgc tcaggctgcc gccgtagtcg
    27061 tcgagcattg cgggttgcag accgacgagg gcaatatgtg ctggcagatg tccgcggata
    27121 tctgccagcg ccagcacttc ggagaaactg ttctggtgca ggctcatttt cttcgcgctg
    27181 agataagccg gaatacgttc tccggcatag gttcgcagcg ttcctggttc cagcccgtag
    27241 tcaatggcat cgagaatcaa cagatggctg gcgctttcga cataccccag caagttcagt
    27301 ccctgagtac cgccatcgac aatctccaca gactcgggcc agtggtaatg ggcatacaac
    27361 cgttccgcca cccgcacgcc gaaaccttca tcggcccaca gcaggttgcc cagccccatt
    27421 accaccacgc gttgctcgct catgaacgct ccttgttact tattttgcca aatttgtggc
    27481 tacggtagcc gttgaccatg gtggagatca ccgtgtcgtc ggacatgatg tcttcacgca
    27541 gcgccatata gacatgaccg atcacaaacg cgccaatcag ccacatcccc agccgatgcc
    27601 agctgtgaat gtccattgag ttgcctcccg tccagtagaa aaattccacc acataacgga
    27661 acggcgcaaa aatagcgtac tggctgtgtt cgctgtacag cgcaaagccg gtgatgatca
    27721 taaagaccga catcaggaaa tagccaaaca tcgccgcctg ggcgatgggg ttatggccta
    27781 tatcggcact cggacgtttt gccagaaaca gataccagcg gatttcatac cacacgccct
    27841 gccaccagct tttacgccat actggcacga taaacagctc gcgggagtat cgattgccaa
    27901 caaaagccca gtagatccgc atcagcaaga ccacggtaaa aaccatcccg gcgctgaagt
    27961 gaattaacct gatgtagccc atatagaaca gatacgtcgc ctcgccgctg acggaaggta
    28021 gcggtttgcc gataaagtaa ccggtgacca tcaacaccgc catgcataac accgtcagcc
    28081 agtgccagat acgcactggc gcttcaaaga catagtggct gacaacgttg tcgcttttct
    28141 gttgcatgat gattctcctt cgctgttaac gcacctgcac ggagatcagc tcgctaccgt
    28201 cgtcgcccag cacgtgtgtt gaacaggcga ggcacggatc aaagctgtgc agagtacgca
    28261 ggatctccag cggttgctcg gggatcgcca ttttggtgtt catcagcgcc gcttcataag
    28321 cgccaatctg ccctttggga tcgcgcgggc tggcgttcca ggtggtcggc accacgcact
    28381 ggtagagatc aatcttgcca tcgcgaatgg cggcccagtg gcctaacgcc ccgcgcggcg
    28441 cttcggtaaa accgacacca cggcactctg tcggccaggt tgtgggttcc catttttccg
    28501 tggaggcggt ggcaagattg ccgtttttca ggttagtcat cagcttgttg aagaaatact
    28561 gcaacttacc tgcggcccac tgtgcttcgt gcgcgcggca caaaatgcgg cccaacgttg
    28621 actggatacc ggaaagcggc aggttcagcg ccgacatcat gcgatcgacc gactcaacgg
    28681 tcgcagcatc gcctttgtga taagcgatta acgtgcgtgc cagcggcccc acttccatcg
    28741 cgttaccgcg ccagcgcggc gctttgatcc acgagtagcg ttcctgttca ttcagctgct
    28801 gaatgttggt atcgctgcct ttgacatcgc cggggttgta ccacgggtcg gtgatgccat
    28861 cgaacggatg acgcccgacc tgatcgttgg gataacgata ccaggcgtga tcgacaaact
    28921 cctgcacctg ctgcggatca accaaatcca ctggcagcac attgttgaag tcgccgttaa
    28981 tcaccgcgcc gccaggcatc agcagacttt tctcaccaaa gtcgttggca atatccggga
    29041 atgcgccgta gctgaggaca catttatcag aaagacccgt gccgatttcg ctccacggtt
    29101 tgttgaactg accgatggct aaggcgtcgg ggatcatcac gttgttaatg aagtctgccg
    29161 tgcgggtgat aatcgactgc accaggttca ggcgttccat attgaccgcc ccgaccgcgc
    29221 cgctttcgtc aatgttaatg gcgcaaggca tcccgccgac aatccagttt ggatgcgggt
    29281 ttttaccgcc aaagaccgcg tggattttga caatttcacg ctggaaatcg agagcttcga
    29341 gatagtgggc aaagcccatc aggttggctt ctggcggcaa cttgtactgc gggtgccccc
    29401 agtagccatt gcggaagatc cccaactgcc cgccttcaac gaatttcttc aggcggtttt
    29461 gtacgtcgaa gaaatagcca ggggatgatt tcggccaaga ggagagactt tgcgccagtt
    29521 cggaggtttt ccgcgggtcg gctttcagcg catccaacac atcgatccag tccatcccgg
    29581 caagctgata gaagtgcacc agatgatcgt ggcaccagag cgttgccagc ataatgttgc
    29641 ggatgatatt ggcgttgtcc ggcactttaa taccgatggc atcttcgatg gcgtaaaccg
    29701 aagccagagc gtgtacgcca gtacagacgc cgcagatacg ttcaacgaac gcccacgcat
    29761 cgcgcgggtc gcgcccttgc aggatgatct ccagcccgcg aaacatggtg ccgcaggaga
    29821 cggcattggt gattacattc tgatcgttaa tattcacttc gcagcgcatg tggccttcaa
    29881 tgcgcgtaat cgggtcgacc accaggcggc gtccggcatt attgatggtg tatccctgag
    29941 tttcgtactg agtgctcatg cctgtttatc ctcattgcct ggctgatgtt cggtttctgt
    30001 aggttgctga ttatgacgtc tgcgctggtc aacggcgctg gcgactgcgt gcacaccaac
    30061 agccgctgcc accacaccaa gcgcggttaa accgacggta tcggcggtgg aatgagtacc
    30121 catttgtgga atatcgacca cgcggctgta gaacgaaccg cgatcccaga aaccattttc
    30181 cgcacagccc aggcagccgt gaccagactg gattgggaaa gaaacgccat cattccagcg
    30241 tgtggaggaa caggcgttat aggtggtagg ccctttgcag cccattttgt acaggcaata
    30301 acctttgcgg gcagcgtcat catcccaact ctggacgaac tctccggcgt cgaagtgagc
    30361 gcggcgatag catttatcgt ggatacgctg accataaaac atcagtggac gacccattct
    30421 gtcgacatct ggcaagcgat caaaggtcac catgtaagta atgatggcgc tcatcacatc
    30481 cgggatcggc gggcagccag gtactttgat aatgggtttg tcggtgatga ctttgtcgat
    30541 agacgttgcc tgtgtcggat taggtcgcgc ggcctgcacg cagccccagg acgcacaggt
    30601 tccccaggcg ataatcgcgc tggctccggc agcggcacgt ttgagtttct caataaacgg
    30661 tcgaccgctg ctgatacaga acatcccctg ctcgcccagc ggcggattac cttctactgc
    30721 gaggatatat ttgccattgt attgcgtgat gatgtcttca aagacttctt ccgcctgggt
    30781 tccggcggca gccatcaaag tatcgtcgta atcgagggaa atcagggaaa ggatgacgtc
    30841 ctttgccagc gggtgagcgg agcggataaa agattcggta cagcaggtgc attccagacc
    30901 gtggatccat accaccggaa tgcgcggttt gttctccagc gcccaggcaa tctttggtgc
    30961 cattcccgcg cctaatccca gcgacgtggc agccagacta caatatttga gaaagctgcg
    31021 ccgggtaacg ccctgacgcc gcatggcctg gtaaaatgtt tcctcgttat tcatatcgca
    31081 cgtctcttct ccttgcgaca ccggcaggac accgggcgct tatggtcggc aagcgacgtc
    31141 cctgtagcaa ggcaaaccat cgcttcacgt gagatgctga aaacgaaagc tcatccttct
    31201 gcactggcgc acgtcgccag aaagtattgt taataaagcg tagtgaaact tttgcacaaa
    31261 acaatacaaa ctgtgtggat ttatctttta gcgataaaaa tggacctatt tttctttttg
    31321 ccgggcggtg ggatgtttag ccagttgcta aacgagtaaa agagaaggaa tttatggtga
    31381 tgaaatgatc aatctgcttt tatttcacgc tatcaatgca cttttggctg tttttcaggc
    31441 aaacaaacag attaggggtt tacacgccgc atcgggatgt ttatagtgcg cgtcattccg
    31501 gaagtgtggc cgagcggttg aaggcaccgg tcttgaaaac cggcgacccg aaagggttcc
    31561 agagttcgaa tctctgcgct tccgccaaat aatcaagggg ttaccaaatg gtagcccctt
    31621 tgcttttttg gctcttggta taaacttggt ataccaattg gtatattttt attcggatga
    31681 gattatcggt catgagcgga acctcaggtt cctcgtctga tgccgctctt gcaacacgtt
    31741 acgctgccga gtatttttgt aaaacatgga ctgctccagg cctgaaccag gcagaaggat
    31801 acaaagccat ctcagatctg tcgcatcact attttcgtgc agaaggctct agccctcccc
    31861 aatctttttt gtgacaccct gtttcactga acatgaagac actcaccttg tccctgatat
    31921 tcattagcat catcctgatt atttaacttc tctatttagg gaagcccgtc tgatacaaag
    31981 cttctattcc tcgttaaata ttctccattc tggcgccaag ccctatctat agctgattga
    32041 aaacaactct aaaaacgtaa taatatcaaa tagtttattc ctacagccga tcattttgag
    32101 taaaatattg acgagaattt cacaaaaggt agatttcatg taaacggata aataagacta
    32161 tccaataaag ctcttataag gttatctata tgataaatag cattaattct ttttttccgg
    32221 ccttcctcgc agtatatcaa gtgctatacg cagcagtacc ttcactgtct cgcagcacaa
    32281 aagcacacct aacacggtaa aaaccagctc acctttttct cccagcaaca gtcctgccag
    32341 tgcaacaact atttttaagg taaaaaattc ttatacagaa tcaggacttc aacgtcccac
    32401 atcttatact cagtcaagta tagaaaaaaa cgctttacat cggcctctac ctgatgtagc
    32461 tcagcgtctg gtgcagcatc ttgcagagca tggcattcaa ccagcccgga gtatggctga
    32521 acatattcct ccggcaccta actggcctgc gccaccaccg ccagtacaaa atgaacaatc
    32581 aagacct
//
LOCUS       DAZKIF010000044        32530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZKIF010000044 DAZKIF010000000
VERSION     DAZKIF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.044"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(94..3243)
                     /gene="acrB"
                     /locus_tag="ACHOTW_002044"
     CDS             complement(94..3243)
                     /gene="acrB"
                     /locus_tag="ACHOTW_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP9844154.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="ACHOTW_002045"
     CDS             complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="ACHOTW_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP9844155.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4601..5248
                     /gene="acrR"
                     /locus_tag="ACHOTW_002046"
     CDS             4601..5248
                     /gene="acrR"
                     /locus_tag="ACHOTW_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP9844156.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPSDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5376..8738
                     /gene="mscK"
                     /locus_tag="ACHOTW_002047"
     CDS             5376..8738
                     /gene="mscK"
                     /locus_tag="ACHOTW_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP9844157.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8777..8941)
                     /gene="rsmS"
                     /locus_tag="ACHOTW_002048"
     CDS             complement(8777..8941)
                     /gene="rsmS"
                     /locus_tag="ACHOTW_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP9844158.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(8955..9482)
                     /gene="priC"
                     /locus_tag="ACHOTW_002049"
     CDS             complement(8955..9482)
                     /gene="priC"
                     /locus_tag="ACHOTW_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP9844159.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9552..9929
                     /gene="ybaN"
                     /locus_tag="ACHOTW_002050"
     CDS             9552..9929
                     /gene="ybaN"
                     /locus_tag="ACHOTW_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP9844160.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10082..10633
                     /gene="apt"
                     /locus_tag="ACHOTW_002051"
     CDS             10082..10633
                     /gene="apt"
                     /locus_tag="ACHOTW_002051"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9844161.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10762..12693
                     /gene="dnaX"
                     /locus_tag="ACHOTW_002052"
     CDS             10762..12693
                     /gene="dnaX"
                     /locus_tag="ACHOTW_002052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9844162.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    12026..12090
                     /gene="dnaX"
                     /locus_tag="ACHOTW_002052"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12746..13075
                     /gene="ybaB"
                     /locus_tag="ACHOTW_002053"
     CDS             12746..13075
                     /gene="ybaB"
                     /locus_tag="ACHOTW_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9844163.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13075..13680
                     /gene="recR"
                     /locus_tag="ACHOTW_002054"
     CDS             13075..13680
                     /gene="recR"
                     /locus_tag="ACHOTW_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9844164.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13790..15664
                     /gene="htpG"
                     /locus_tag="ACHOTW_002055"
     CDS             13790..15664
                     /gene="htpG"
                     /locus_tag="ACHOTW_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP9844165.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15845..16489
                     /gene="adk"
                     /locus_tag="ACHOTW_002056"
     CDS             15845..16489
                     /gene="adk"
                     /locus_tag="ACHOTW_002056"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9844166.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16621..17583
                     /gene="hemH"
                     /locus_tag="ACHOTW_002057"
     CDS             16621..17583
                     /gene="hemH"
                     /locus_tag="ACHOTW_002057"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP9844167.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17580..18539)
                     /gene="aes"
                     /locus_tag="ACHOTW_002058"
     CDS             complement(17580..18539)
                     /gene="aes"
                     /locus_tag="ACHOTW_002058"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP9844168.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18691..19995
                     /gene="gsk"
                     /locus_tag="ACHOTW_002059"
     CDS             18691..19995
                     /gene="gsk"
                     /locus_tag="ACHOTW_002059"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP9844169.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20128..21804)
                     /gene="ybaL"
                     /locus_tag="ACHOTW_002060"
     CDS             complement(20128..21804)
                     /gene="ybaL"
                     /locus_tag="ACHOTW_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP9844170.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22041..23261)
                     /gene="fsr"
                     /locus_tag="ACHOTW_002061"
     CDS             complement(22041..23261)
                     /gene="fsr"
                     /locus_tag="ACHOTW_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP9844171.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23479..25131
                     /gene="ushA"
                     /locus_tag="ACHOTW_002062"
     CDS             23479..25131
                     /gene="ushA"
                     /locus_tag="ACHOTW_002062"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9844172.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25168..25647)
                     /gene="ybaK"
                     /locus_tag="ACHOTW_002063"
     CDS             complement(25168..25647)
                     /gene="ybaK"
                     /locus_tag="ACHOTW_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP9844173.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(25851..26645)
                     /gene="ybaP"
                     /locus_tag="ACHOTW_002064"
     CDS             complement(25851..26645)
                     /gene="ybaP"
                     /locus_tag="ACHOTW_002064"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP9844174.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26783..27124
                     /gene="ybaQ"
                     /locus_tag="ACHOTW_002065"
     CDS             26783..27124
                     /gene="ybaQ"
                     /locus_tag="ACHOTW_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9844175.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27339..29843)
                     /gene="copA"
                     /locus_tag="ACHOTW_002066"
     CDS             complement(27339..29843)
                     /gene="copA"
                     /locus_tag="ACHOTW_002066"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP9844176.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30105..31037
                     /gene="glsA"
                     /locus_tag="ACHOTW_002067"
     CDS             30105..31037
                     /gene="glsA"
                     /locus_tag="ACHOTW_002067"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9844177.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31040..32332
                     /gene="ybaT"
                     /locus_tag="ACHOTW_002068"
     CDS             31040..32332
                     /gene="ybaT"
                     /locus_tag="ACHOTW_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9844178.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKRMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         7890 a         8583 c         8543 g         7514 t
ORIGIN      
        1 gagatcctga gttggtggtt caattactcc ttaatgttcg taggttatgc ataaaaaagg
       61 ccgcttacgc ggccttagtg attacacgtt gtatcaatga tgatcgacag tatggttgtg
      121 ctcgatatct tcattcttgc ggctaaagcg gcggcgaacc accacaaaga ataccggaac
      181 gaagaagatt gccagtaccg ttgcggtcac catcccgccc attacaccgg tacctactgc
      241 gttctgcgcg ccggaaccag caccagtact gataaccagc ggcataacgc cgaggataaa
      301 cgccagcgag gtcatcagga tcggacgtaa acgcatccgc accgcatcaa gcgtcgcttc
      361 aatcagacct ttaccttctt tatccatcaa gtctttggcg aattcgacga taagtatcgc
      421 gttcttcgcc gacaacccaa tggttgtgag caggcctacc tggaagtaaa cgtcattggt
      481 caggccacgg aaggtggcag ccagcaacgc accgataacc cccagcggaa cgaccagcat
      541 aacggagaac ggaatcgacc agctctcgta cagcgccgcc agacacagga acacgacaat
      601 caacgaaatc gcgtacagtg aaggtgcctg gttgccggag agacgttcct gataggacat
      661 ccccgtccag tcatagccaa caccggtagg cagtttgctc gccagttgtt ccatcagctc
      721 cattgcttca ccggtacttt tacccggtgc cgcctggcct aagatttcca tggatggcag
      781 gccgttgtaa cgttccagac gcggcgaacc gtactcccaa cgagaagagg agaacgccga
      841 gaatggcacc atctgaccat cagcagcacg aacataccag tcgccgatat catccggcag
      901 catacggtat ttcgcttctg acatgacgta aactttcttc acacgaccgc ggtcgataaa
      961 gtcgttcaca tagctgccgc cccatgcagc gcccagagtg gtgttaatgt cgttgataga
     1021 aacacccagc gcctgcgctt tttcctggtc gatatcaatc ttaaactgcg gggtatcttc
     1081 cagaccgttt ggacgtacgc tggtcaacat atcagggtgc ttcgctgctt ctgcaagcaa
     1141 ctggttacgc gcctgagtca gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa
     1201 gtcaaagccg gtggcggtac ccagttccac gattgcgggc aggttaaagg cgaaaaccat
     1261 cgcatctttg atttgcgaga aagcgcgtgt tgcacgcatg gtaatcgctt caactttgtt
     1321 ttcttcaccc ggacgatcgg cccagtcctt caaggaaacg aacgcaatac cggtattctg
     1381 accacgtccc gcaaagccga agccgttaac ggcgaacacc gactcaacgt tgttcttttc
     1441 tttggtcaga tagtaatgcg ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc
     1501 acctgctggc agctgaacca tggtcataaa tacgccctgg tcctcatctg gcaagaagga
     1561 gcttggcaga cgcacaaaca gataggccat gccgaccacg atgatcagat acagcaccag
     1621 ataacgcccc gtactgcgca gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt
     1681 ctcgaacatg cggttaaacc agccgaagaa gcctttttta ccttccccgt gatcgccttt
     1741 ggcaatcggt ttcagcatgg tggcacaaag agctggagtc aggatcaacg ccaccagtac
     1801 cgacagcgcc attgctgaaa caatggtaat agagaactga cgataaattg ccccagtaga
     1861 accgccaaag aaggccatcg gtacgaatac cgccgacagt accatcgcga taccgaccag
     1921 agcgccctga atctgcccca tcgacttacg ggtggcttct tttggcggca aaccttcttc
     1981 cgccatcaca cgctcaacgt tttctaccac aacgatagcg tcatccacca acaggccgat
     2041 ggcgagcacc atcccgaaca ttgttagcgt gtttatcgag aagccaaagg cggcaaggac
     2101 ggcaaaggtc ccgagcaata ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa
     2161 gttctgcagg aacagataca taaccaggaa cacgaggatg atcgcttcga ccagcgtttt
     2221 aaccacttcg tgaatagaga ttttcacgaa cggcgtggtg tcgtacgggt aaacaatttt
     2281 cagacccgac gggaagaacg gttccatctt cgccagttca gcacggattg ccgcagcggt
     2341 atccagcgcg tttgcaccgg tcgccagctt gatccccaga ccggaagccg gttggccgtt
     2401 aaactctgcg atgatgtcgt agttctcacc acccagctca atcttcgcca catcacgcag
     2461 cagtacgcgg gaaccatcct gattcacttt cagcaggatt ttgccgaact cttcagtaga
     2521 ggtcagacgc gtctgagcaa taatagaggc gttaagctgt tggcctttca ccggcggtgt
     2581 accaccgagc tggcccgccg caacctgggc gttctgcgct ttgatggcgg taataacatc
     2641 aaccggcgtt agctggaatt tgttcagttc attcgggttc atccagatac gcatcgcgta
     2701 ctgtgaaccg aacaactgaa cgtcacccac gccagacgta cggctgatgg catctttcat
     2761 attcgccgcc acgtagtcgg agatatcctc ctgcgtcatg gtgccatcgg tgttgataac
     2821 gccgacaacc atcaggaagc tgctggatga tttctcaatg ctcacccctt gctgctgaac
     2881 ttcttgcggc agcaacggca tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat
     2941 atccgcatca gtaccagact caaaggtcag ggtgatctgt acggtacccg tggagtcact
     3001 gttagaggac atgtacatca ggttatcgat accgttcata ttctgttcga taacctgtgt
     3061 caccgtgtcc tgcactgttt tcgcatcagc gccggggtag gaggcggaga tcgttactgc
     3121 cggcggtgca atcgtaggat attgcgccac cggcagtttg aggatcgcca gcccccctgc
     3181 caacatgatg ataatggcga tcacccacgc aaaaatcggg cgatcgataa agaaattagg
     3241 catgtcttaa cggctcctgt ttaagttaag acttggactg ttcaggctga gcaccgcttg
     3301 cggcttgctg gttattatca gcggtaactt cttgtgcttt tacctggaca ccaggacgca
     3361 ctttctgcag cccacttatt actacgcgat cgcctgcttt cagaccttct gtcaccagcc
     3421 acttatcgcc aatagcctgg cttgcaacga tcggacgggt ttccactttg tcatccgcgc
     3481 caaccaccag tacggtggca tcgccacgcg gcgtacgggt tacgccctgt tgcgggacta
     3541 aaatagcgtt tggattaagc ccttcttcca gacgtgcgcg cacgaacata cccggcagca
     3601 gagtgtgatc cgggttcggg aagatagcgc gtagggtgat agacccagtg gtctgatcaa
     3661 cggtaacgtc agagaattcc agcgtaccgt cctgcgggaa cttaatgccg tcactggtga
     3721 tcagcgacac tttggctttg ccgttctctt gtttcagcgt gccattcgcc agttcctgtt
     3781 tcaggcgcag gaagtcgttg ctggactggg tcacatcaac gtagatcgga tcaagttgct
     3841 gcacggttgc cagcgcagtc gcctgaccgt tctgtaccaa tgcgccttcc gtcacgttcg
     3901 acttaccaat gcgaccgcta atcggagagg tgactttggt gtaagccaga ttgatccgcg
     3961 cagtttcaac ggcagctttc gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca
     4021 gagcctgatc gtactcttgc ttactgatgt actgagtacc gagcagtttc tgataacgat
     4081 tcaccgtcaa ttgcgcgata ttggctgcag cctgggcttt cgccagatca cctttcgcac
     4141 tgtcgtatgt cgcctgatag gtcgcaggat caatctgata gagagagaca cctgcttcga
     4201 tgtcgctacc ttctttgaaa ttacgcttca ggataatccc gctaacttga ggacgaactt
     4261 ctgcgatccg gtaggcactg gtgcgacccg gaagctcggt tgtgatctgc agaggttcag
     4321 ttttgactgt tactacgcca acggcgggca tctgctggcc accttgttgg gcctgtttgt
     4381 cgtcacatcc tgttagggct aagctgcctg agagcatcag aacgaccgcc agaggcgtaa
     4441 accctctgtt tttgttcata tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc
     4501 aaaagttaat aaacccattg ctgcgtttat attatcgtcg tgctatggta catacattca
     4561 caaatgtatg taaatctaac gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca
     4621 agaagcgcaa gaaacgcgcc aacacatcct cgatgtggct ctacgtcttt tctcacagca
     4681 gggggtatca tccacctcgc tgggtgagat tgcaaaagca gctggcgtta cgcgcggtgc
     4741 aatctactgg cattttaaag acaagtcgga tttgttcagt gagatctggg aactatcaga
     4801 atccaatatt ggtgaactag agcttgagta tcaggcaaaa ttccctagcg atccactctc
     4861 agtattaaga gagatattaa ttcatgttct tgaatccacg gtgacagaag aacggcgtcg
     4921 attattgatg gagattatat tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca
     4981 acaggcacaa cgtaatctct gtttggaaag ttatgaccgt atagaacaaa cgttaaaaca
     5041 ttgtattgaa gcgaaaatgt tgcctgcgga tttaatgacg cgtcgggcag caattattat
     5101 gcgcggctat atttccggcc tgatggaaaa ctggctcttt gccccgcaat cttttgatct
     5161 taaaaaagaa gcccgcgatt acgtcgccat cttactggag atgtatctcc tgtgccccac
     5221 gcttcgtaat cctgccacta acgaataacc ctgaatctga ctccaggatt tttcctggac
     5281 attttcgtcg ttgctattct ggttcactgc gtcgtgatat tcttgcggtt tgactttttc
     5341 aggtcgttct tcaggttcag aaaccttcat tcatcatgac tatgttccag tattacaaac
     5401 gatcacggca ttttgttttt tcagcattta ttgcttttgt ttttgtcttg ttatgccaga
     5461 acacggcgtt tgcgcgggcg tcatcgaatg gtgatctgcc gacaaaagcg gacctgcagg
     5521 cgcaacttga ctcactaaat aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc
     5581 aggatctgac agatacatta gccaccctcg ataaaatcga tcgcgtaaaa gaagagaccg
     5641 ttcagctacg gcaaaaagtc gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt
     5701 taacagcact tagcgatgtc gataacgacg aagaaacgcg caaaattctg agcacgcttt
     5761 cgttgcgcca gctggaaact cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa
     5821 acgatctggc gtcttataac agccagctgg tttcgttaca gacgcagccc gaacgcgtgc
     5881 aaaatgcgat gtataacgct tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga
     5941 ctgatgtcgg cgagacggcc ttacgtccca gccagaaagt gttaatgcag gctcagcagg
     6001 cgttgctgaa tgcggagatt gaccagcagc gtaaaagcct ggaagggaat accgtcttgc
     6061 aggatacctt gcaaaagcaa cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc
     6121 agttgcagct gttgcaagaa gcggtaaaca gcaagcgcct gactttaacc gaaaaaacgg
     6181 cgcaggaagc cgtctccccg gatgaagccg cgcgtattca ggctaatccg ctggtgaagc
     6241 aggaactgga aattaaccag cagttaagtc agcgtctgat taccgcgact gaaaacggta
     6301 atcagttgat gcagcaaaac attaaagtca aaaactggct ggagcgggcg ctgcaatcgg
     6361 aacgcaatat taaagagcag attgccgtcc tgaagggcag cctgctgttg tctcgtatcc
     6421 tttaccagca acaacaaacg ctgccctcgg cggatgaact ggaaaacatg accaaccgca
     6481 tcgcggattt gcgtctcgaa cagtttgaag ttaaccagca gcgtgatgca ctcttccaga
     6541 gcgatgcgtt cgtcaacaaa ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc
     6601 acgatgcgtt attgcaagtg gttgatatgc gtcgcgaatt gctggatcaa ctcaacaaac
     6661 agttgggtaa ccagctgatg atggccatta acctgcaaat caaccagcag cagttaatga
     6721 gtgtgtcgaa aaacctgaaa tccatcctga ctcagcaaat cttttgggtg aacagtaacc
     6781 gtccaatgga ctgggactgg atcaaagcgt tcccgcaaag cctgaaagat gaatttaagt
     6841 cgatgaaaat tacggttaac tgggaaaaag cctggcccgc cgtttttatc gctttcctcg
     6901 ctggtttgcc gctgctgttg attgccgggc tgatccactg gcgtctgggg tggctgaaag
     6961 cgtatcaaca aaaactggct tccgctgtgg gttccctgcg taacgacagc cagctcaaca
     7021 caccaaaagc gatccttatc gacctgatcc gtgcactgcc ggtgtgcctg attattctcg
     7081 cggttggcct gattctgttg accatgcagc tcaacatcag cgaactgcta tggtcgttca
     7141 gcaaaaaact ggcgattttc tggctggtgt ttggcctgtg ctggaaagtg ctggagaaga
     7201 acggcgttgc cgtgcgtcac ttcggcatgc cggaacagca gaccagccac tggcgtcggc
     7261 aaattgtccg catcagtctc gcgttgctgc ctatccattt ctggtctgtg gtggcagaac
     7321 tttccccgct gcatctgatg gatgatgtgc tggggcaggc gatgattttc ttcaacctgc
     7381 tgctgattgc cttcctggta tggccaatgt gccgcgaaag ctggcgtgat aaagagtcgc
     7441 acaccatgcg actggtcacc attaccgtgc tgtcgattat cccgattgca ttgatggtgc
     7501 tgactgccac gggttatttc tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg
     7561 tttatctggt gatcatctgg aacttgctct accagacggt attgcgcggc ttaagcgtag
     7621 cggcgcggcg tatcgcctgg cgtcgtgcgc tggcgcgtcg gcagaatctg gtgaaagagg
     7681 gcgcagaagg tgccgaaccg ccggaagaac ccaccattgc actggaacag gttaaccagc
     7741 agacgctgcg tattaccatg ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa
     7801 tttggtccga tttgatcacc gtgttcagct atctcgacag cattacgctg tggcattaca
     7861 acggtactga agctggtgct gcggtggtga aaaacgtcac catgggtagt ctgttgtttg
     7921 cgattatcgc ctcaatggtg gcctgggcgt tgattcgcaa cctgcctggt ttgctggaag
     7981 tgctggtgct ctcgcgactg aatatgcgcc agggcgcgtc gtatgccatt actaccatcc
     8041 ttaactacat cattattgct gttggtgcga tgacggtgtt cggatcgctg ggcgtctctt
     8101 gggataaact ccagtggctg gccgcagcat tatccgtagg tcttggtttt ggtttacaag
     8161 aaattttcgg taacttcgtc tccggtttga tcattctgtt cgaacgtccg gtgcgtattg
     8221 gcgatacggt aaccattggt agcttctcgg ggacggtaag taaaatccgt attcgtgcga
     8281 caacgattac cgatttcgat cgcaaagaag tgatcatccc gaacaaagcg tttgttaccg
     8341 agcgtctgat caactggtcg ttgactgaca ctactacgcg tctggtgatc cgtctcggcg
     8401 tggcctatgg ctccgatctg gaaaaagtgc gtaaagtgtt actgaaggcg gcgactgagc
     8461 acccaagggt gatgcacgaa ccaatgccgg aagtcttctt tacggcattt ggtgccagca
     8521 cgttggatca tgagctgcgt ctgtatgtgc gtgaactgcg tgaccgtagt cgtactgtcg
     8581 atgagctgaa ccgtactatc gatcagctgt gccgtgaaaa cgacatcaac attgccttta
     8641 accagcttga agtgcatctg cacaacgaga agggcgatga ggtgacggaa gtaaaacgcg
     8701 actacaaagg cgacgatccg acgccagcgg tagggtaagg acaataaagg gcgacagtga
     8761 tgccgccctt tattttttac tcgctttgtg aagcctcatc gctctgtaac ttcttttcat
     8821 aatcacgctt tacaatatcc agcgcgcgca gcacggtgcg ggctgggatc tgattttctt
     8881 ccagcagcac aatcaaatcg acagccagtt tgacatcgtc aggggcattt tccagtgaca
     8941 tactctctcc attgctagcg ggttaaacgc gctaacctgt tttcgatttt ttccagcgca
     9001 tggcggcagc gtgccaggcg cgcttcatag gcttccactt cacgatgcag cgtttgctgt
     9061 tccacgagat cggtcacccg cgccaggcgg gctctacgtt cggcaaccat ctcacgtagc
     9121 cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc agcgggcaat ttggggtggt
     9181 gcgctatccc actcgcgtag cgaccaggct gtggcttcac gcgctatagc ctccagttgc
     9241 gccgccagat gttctgccag ccaggcgact tgcggcagtt gctgttgctc aactgcatga
     9301 cgaagcgcag ccagattatc gcccgccttg tcgagacaag cctgtagtgt tgtcgcacga
     9361 gtctgaaaaa gatgcctgtt gaaacgagca cttagcgtgg cgaactgtga caccggggca
     9421 caacgctgac gcagcgtagc gagctgtcct tccagttttt ccagcagcag ggcggttttc
     9481 acgataagac ctcaatgaaa atgataattg ttatgctaaa gtagccactc tgtaagctga
     9541 caacactcaa tatgcaacga atcattttaa tcatcattgg ctggctggcg gtagtgctgg
     9601 gcacgctggg cgtggtatta ccggtattac cgacgacgcc gtttatcctg ctggcggcct
     9661 ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt gctgtaccgc tcatggtttg
     9721 gcagctatct acgtttctgg cagaaacatc atgcgatgcc gcgcggcgtc aaaccgcggg
     9781 cgattttgct tattttgctc acgtttgcca tttctctgtg gttcgtccag atgccatggg
     9841 tgcgcatcat gttgctggta attctcgcct gtttgctttt ctatatgtgg cgaattccgg
     9901 tgattgatga aaagcaagaa aagcactgaa gcacaacaat cgcagttgca attattgcgt
     9961 acagccagta cattctggcg ttttcgagca caggcgcagg cggtcaaagg ttaaacaact
    10021 gttacttttg atacgtttaa aacgcgccgt gagtaccacc gtaacaagca ggcatacact
    10081 tatgaccgcg actgcacagc agcttgagta tctcaaaaat agcatcaaaa gcattcagga
    10141 ctacccaaaa cccggcattc ttttccgcga tgtcaccagc ttactggaag acccgaaagc
    10201 ttacgctctc agcatcgact tgctggttga gcgttacaaa aatgcgggca ttaacaaagt
    10261 tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt
    10321 tggctttgta ccggtccgta aaccgggcaa actgccgcgt gaaaccatca gtgaaactta
    10381 cgatctggaa tacggcaccg atcagctgga gatccacgtt gatgccatca aaccgggcga
    10441 caaagttctg gtggtggacg atctgctggc aaccggcggc actatcgaag cgaccgttaa
    10501 actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg ttcattatca acctgttcga
    10561 tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt
    10621 cccgggccat taattatcgc cagtcttgtg ctgcccacgc tacggacagc acaagatgtg
    10681 cattcagcct cgccgttctg acggggctgt gttagcatta ccccttcgag aatccacctt
    10741 ccagcgtttc agagcctgcc aatgagttat caggtcttag cccgaaaatg gcgcccacaa
    10801 acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg
    10861 ttagggcgta ttcatcatgc ttatcttttt tccggcaccc gtggcgtcgg aaaaacctct
    10921 atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc
    10981 ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct ttgtcgatct gattgaaatc
    11041 gacgccgcct cgcgcaccaa agttgaagat acccgcgacc tgctggataa cgtccagtac
    11101 gctccggcgc gtggtcgttt caaagtttat ctcatcgacg aagtgcatat gctgtcgcgc
    11161 cacagcttta acgcactgtt aaaaaccctt gaagagccgc cggagcacgt taagtttctg
    11221 ctggcgacga ctgatccaca gaaattgccg gtgacgattt tgtcgcgctg tttgcaattt
    11281 catctcaagg cgctggatgt cgagcaaatt cgtcatcaac ttgagcacat cctcaatgaa
    11341 gaacatatcg ctcacgagcc gcgggcgctg caattgctgg cgcgcgccgc tgaaggcagc
    11401 ctgcgagatg ccttaagtct gaccgaccag gcgattgcca gcggtgacgg ccaggtttca
    11461 acccaggcgg tcagtgcgat gctgggtacg cttgacgacg atcaggcgct gtcgctggtt
    11521 gaagcgatgg tcgaggccaa cggcgagcgc gtaatggcgc tgattaatga agccgctgcc
    11581 cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg gcctgttgca tcgtattgcg
    11641 atggtacaac tttcgcctgc tgcacttggc aacgacatgg ccgccatcga gctgcggatg
    11701 cgtgaactgg cgcgcaccat accgccgacg gatattcagc tttactatca gacgctgttg
    11761 attggtcgca aagaattacc gtatgcgccg gaccgccgca tgggcgttga gatgacgctg
    11821 ctgcgcgcgc tggcgttcca tccgcgtatg ccgctgcctg agccagaagt gccacgccag
    11881 tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa cccaggtgcc gccgcaaccg
    11941 caatcagcgc cgcagcaggc accgactgta ccgctcccgg aaaccaccag ccaggtgctg
    12001 gcggcgcgcc agcagttgca gcgcgtgcag ggagcaacca aagcaaaaaa gagtgaaccg
    12061 gcagccgcta cccgcgcgcg gccggtgaat aacgctgcgc tggaaagact ggcttcggtc
    12121 accgatcgcg ttcaggcgcg tccggtgcca tcggcgctgg aaaaagcgcc agctaaaaaa
    12181 gaagcgtatc gctggaaggc gaccactcca gtgatgcagc aaaaagaagt ggtcgccaca
    12241 ccgaaggcgc tgaaaaaagc gctggaacat gaaaaaacgc cggaactggc ggcgaagctg
    12301 gcggcagaag ccattgagcg cgacgcgtgg gcggctcagg tgagtcaact ttcgctacca
    12361 aaactggtcg aacaggtggc gttaaatgcc tggaaagagg agagcgacaa cgcagtatgt
    12421 ctgcatttgc gctcctctca gcggcatttg aacaaccgcg gtgcacagca aaaactggct
    12481 gaagcgttga gcatgttaaa aggttcaacg gttgaactga ctatagttga agatgataat
    12541 cccgcggtgc gtacgccgct ggagtggcgt caggcgatat acgaagaaaa acttgcgcag
    12601 gcgcgcgagt ccattattgc ggataataat attcagaccc tgcgtcggtt cttcgatgcg
    12661 gagctggatg aagacagtat ccgccccatt tgatcgtaag cacagcttac gttcgtcatc
    12721 cttaacgtga ttgagagaga aacctatgtt tggtaaaggc ggtctgggta acctgatgaa
    12781 gcaagcccag cagatgcaag aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga
    12841 agttaccggc gaatctggcg caggtctggt aaaagtgacc atcaacggtg cacataactg
    12901 ccgtcgcgta gagatcgacc cgagcctgct ggaagacgac aaagagatgc tggaagacct
    12961 ggtggctgca gcattcaacg acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat
    13021 ggcttctgta tcctccggaa tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa
    13081 accagcccgc tgttaacaca gcttatggaa gcactgcgct gtctgccggg cgttggcccg
    13141 aagtcggcgc agcgtatggc gttcacgctg cttcagcgcg atcgtagcgg cgggatgcgt
    13201 ctggcgcagg cgctcacccg ggcgatgtcg gaaatcggcc actgcgccga ttgccgcacc
    13261 ttcaccgaac aggaagtctg taacatctgt tcgaatccgc gtcgtcagga aaacggtcaa
    13321 atctgcgtgg tggagagtcc ggcggacatt tacgccattg agcagacggg gcagttttca
    13381 ggtcgttatt ttgtgttgat gggacatctg tcaccgctgg acggcatcgg tccggatgat
    13441 atcggccttg atcgtctgga acagcgtctg gcagaggaaa aaatcactga agtgatcctc
    13501 gccaccaacc ctacggttga aggtgaagct accgctaact acattgccga gctttgcgcg
    13561 caatatgacg tggaagccag ccgaatcgct catggcgtac cggttggcgg cgaactggaa
    13621 atggtcgatg gcaccacgct gtcacactcc cttgccgggc gtcataagat tcgtttttaa
    13681 gcaaacgaga gcaggatcac ctgctctcgc ttgaaattat tctcccttgt ccccatctct
    13741 ccctcatcct gtttttaacc ttaaaatggc attattgagg tagacctaca tgaaaggaca
    13801 agaaactcgt ggttttcagt cagaagtgaa acagcttctg cacctgatga tccattctct
    13861 ctattccaat aaagaaatct tcctgcgtga gcttatctct aacgcctccg atgcggcgga
    13921 caagttgcgt ttccgtgcgc tctctaaccc ggacctgtac gaaggtgatg gcgaactgcg
    13981 cgttcgtgtc tctttcgata aagacaagcg cacgctgacc atctccgata acggcgtggg
    14041 gatgacccgc gacgaagtga ttgaccatct ggggactatc gctaaatccg gtaccaaatc
    14101 attcctcgaa tccctgggtt ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg
    14161 tgttggtttc tactctgcgt ttatcgtggc cgacaaagtg accgtgcgta ctcgcgcggc
    14221 aggcgaaaaa ccagaaaacg gcgtcttctg ggaatcggct ggcgaaggtg aatataccgt
    14281 tgccgacatc accaaagaag atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga
    14341 agacgagttc ctcgatgact ggcgcgtgcg ttccatcatc agcaaatact ccgaccatat
    14401 cgcgctgccg gtagagatcg aaaaacgcga agagaaagac ggcgaaaccg ttatctcctg
    14461 ggagaaaatt aacaaagcgc aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga
    14521 agagtacaaa gagttctaca aacacatcgc ccacgatttt aacgatccgc tgacctggag
    14581 ccacaaccgt gttgaaggta agcaggagta caccagcctg ctgtacattc cgtcccaggc
    14641 tccgtgggat atgtggaacc gcgatcataa acacggcctg aaactgtatg ttcagcgtgt
    14701 gttcatcatg gacgacgcag aacagttcat gccgaactat ctgcgcttcg tgcgtggtct
    14761 gattgactcc agcgatctgc cgctgaacgt ttcccgtgaa atcctccagg acagcacggt
    14821 aacgcgtaac ctgcgcaatg cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc
    14881 gaaagacgac gcggaaaaat accagacctt ctggcaacag tttggcctgg tactgaaaga
    14941 aggtccggcg gaagatttcg ctaaccagga agcgatcgcc aaattgctgc gttttgcttc
    15001 tacccatacc gattcttctg cgcagaccgt gtctctggaa gactacgttt cccgcatgaa
    15061 agaagggcag gagaaaatct actacatcac tgcagacagc tatgcggcag cgaagagcag
    15121 cccgcacttg gaactgctgc gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat
    15181 cgatgagtgg atgatgaact atctgactga gttcgacggt aaaccgttcc agtcggtgtc
    15241 taaagttgac gagtcgctgg aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc
    15301 ggagaaagca ctgactccgt tcatcgaccg tgtgaaagcc ctgctcggcg agcgcgtgaa
    15361 agatgtccgt ctgactcacc gtctgaccga tacgccagcg atcgtttcga ccgacgcgga
    15421 cgaaatgagc actcagatgg cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt
    15481 gaaatacatc ttcgaactga acccggatca cgtactggtg aaacgtgcgg cagatactga
    15541 agatgaagcg aagttcagcg agtgggtaga actgctgctg gatcaggcac tgctggcaga
    15601 acgcggcacg ctggaagatc cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc
    15661 ctgatgtaat gccggatgac cttcgtgtca tccggcattt ttcttttcat tatctgcact
    15721 ttccgcaaat tatctcgcca ttaaccgttt cagcccctgg tgcctttctt gaggcaatcg
    15781 cctgatggtg gtatcgttta tcgctttttc aaaaaattcg acacatttta aggggatttt
    15841 cgcaatgcgt atcattctgc ttggcgctcc gggcgcgggg aaagggactc aggctcagtt
    15901 catcatggag aaatatggta ttccgcaaat ctccactggc gatatgctgc gtgctgcggt
    15961 caaatctggc tccgagctgg gtaaacaagc aaaagacatt atggatgctg gcaaactggt
    16021 caccgacgaa ctggtgatcg cgctggttaa agagcgcatt gctcaggaag actgccgtaa
    16081 tggtttcctg ttggacggct tcccgcgtac cattccgcag gcagacgcga tgaaagaagc
    16141 gggcatcaat gttgattacg ttctggaatt cgacgtaccg gacgaactga tcgttgaccg
    16201 tatcgtcggt cgccgcgttc acgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc
    16261 gccgaaagta gaaggtaaag acgacgttac cggtgaagaa ctgactaccc gtaaagacga
    16321 tcaggaagaa accgtacgta aacgtctggt tgaataccat cagatgacag caccgctgat
    16381 cggctactac tccaaagaag cggaagcggg taacaccaaa tacgcgaaag ttgacggcac
    16441 caagccggtt gctgaagttc gcgctgatct ggaaaaaatc ctcggctaat caagccgcag
    16501 cagatctgct ttcgggcagg tctgctcatc cacctcaggc aatacctctc acagcaatta
    16561 gttcttcttc ctcacttttc cgctacaatt atcaacaagt tgaatcgata agaggcggta
    16621 atgcgtcaga ctaaaaccgg tatcctgctg gcaaacctgg gtacgcccga tgcccccaca
    16681 cctgaagcgg taaaacgcta tctgaaacaa tttttaagcg acagacgcgt ggttgatacc
    16741 tcacggttgt tatggtggcc attgctgcgc ggcgtgattt tgccgctgcg ctcgccgcgt
    16801 gtggcgaagc tgtatgcctc tgtctggatg gaaggtggct cgccgctgat ggtttacagc
    16861 cgccagcaac agcaggcgct ggcacaacgt ttaccggaga cgcccgtagc gctgggaatg
    16921 agctacggct cgccatcact ggaaagcgcc gtagatgaac tcctggcaga gcatgtagat
    16981 catattgtgg tgctgccgct ttatccgcaa ttctcctgtt ctacggtcgg tgcggtatgg
    17041 gatgaactgg cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttatacgt
    17101 gattacgccg ataaccacga ttacattaat gcactggcga acagcgtacg cgcttctttt
    17161 gccaaacatg gcgaaccgga tctgctgctg ctctcttatc atggcattcc ccagcgttat
    17221 gcagatgaag gcgatgatta cccgcaacgt tgccgcacaa cgactcgtga actggcttcc
    17281 gcattaggga tggcaccgga aaaagtgatg atgacctttc agtcgcgctt tggtcgggaa
    17341 ccctggctga tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtaggt
    17401 catattcagg tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt
    17461 gccgagcaaa accgtgaggt cttcctcggt gccggcggga aaaaatatga atatattccg
    17521 gcgcttaatg ccacaccgga acatatcgaa atgatggcta atcttgttgc cgcgtatcgc
    17581 taaagctgag cggtaaagaa ctgagcgccg tcgcgaagag cctcgtcggc ggttttcatc
    17641 atccgtgaat aatgcaaaaa ggcgtgcagc gtgcctgggt agagtttgaa ctcacagggc
    17701 tgctgatgcg ccgctaacgt ctggtaaagc agacggctgt catccagcag cggatcgaac
    17761 tccgccccgg caataaaaca gggcggaact tcgcgagtga gatcattatt aaacagacag
    17821 taatacggcg actcgcggtc cgcgtcgttg cttaaatacg cctcttcata catctgcaaa
    17881 tcctgttgcg ttaagccatc ccagacaccg cccaacagac gacgagtcac ggaatcccgt
    17941 aatccgtaaa gcccatacca cagcaaaacg cccgcaactt taccgcaatc gatctgttta
    18001 tcacgcaacc acaacgcact ggcgagcgcc agcatggcac ctgcggaatc accggcaaag
    18061 ccaatgcggg acatattgat ttgataatcc tccgcctgct ggtggaaata acaacaagca
    18121 gccacaattt cctctatcac ttgcggaaaa cgcgcttcag gtgaaagggt gtaatcaata
    18181 ccaatcaccg tacattggct gtagcttgcc agcaggcgca tgatgcgatc gtgggtatcg
    18241 agattgccga gaataaaacc gcctccatgc aaataaaata gcgttgccgg gctatctggc
    18301 tgcggacaaa agagacgtgt ttccacctgc ccatattttg ttggaaccat gtaagctttg
    18361 gttgccattt ctggagcgcc cgcattccag aatcggcgct caagcgtgta atactgtcgt
    18421 tgctcagcaa tcgttcccgt tgcgggccag ggcggtaaat ccggctgaag agtattcaca
    18481 acggtcttca tttcagcaga aataaggtcc agaacaggta atttgttttc cggcttcata
    18541 caaaaactcc tttcaaatta cgtcattgta aggaaccact gccatgaaaa tgcgatcccg
    18601 cctgctgata ttgaaactgg ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg
    18661 ccgctcagtt aaccacccgt aaaaacaacc atgaaatttc ccggtaaacg taaatccaaa
    18721 cattacttcc ccgtaaatgc acgcgatccg ctgcttcagc aattccagcc agaaaacgaa
    18781 accagcgccg cctgggtagt gggtatcgat caaacgctgg tcgatattga agcgaaagtg
    18841 gatgatgaat tcattgagcg ttatggatta agcgccgggc attcactggt gattgaggat
    18901 gatgtagccg aagcgcttta tcaggaacta aaacagaaaa acctgattac ccatcagttt
    18961 gcgggtggca ccattggtaa caccatgcat aactactcgg tgctcgcgga cgaccgttcg
    19021 gtgctgctgg gcgtcatgtg cagcaatatt gaaattggca gctatgccta tcgttacctg
    19081 tgtaacacct ccagccgtac cgatcttaac tatctacaag gcgtggatgg cccgattggt
    19141 cgttgcttta cgctgattgg cgagtccggg gaacgtacct ttgctatcag ccctggccac
    19201 atgaaccagc tgcgggctga aagtattccg gaagatgtga ttgccggagc ctcggctctg
    19261 gttctcacct cttatctggt gcgttgcaag ccgggtgaac ccatgccgga agcaaccatg
    19321 aaagccattg agtacgcgaa gaaatataac gtaccggtgg tgctgacgct gggaactaag
    19381 tttgtcattg ccgagaatcc acagtggtgg cagcaattcc tcaaagacca cgtctctatc
    19441 cttgcgatga acgaagatga agccgaagcg ttgaccggag aaagcgatcc gttgttggca
    19501 tctgacaagg cgctggactg ggtagatctg gtgctgtgca ccgccgggcc aatcggcttg
    19561 tatatggcgg gctttaccga agacgaagcg aaacgtaaaa cccagcatcc gttgctgccg
    19621 ggcgctatag ccgaattcaa ccagtatgag tttagccgcg ccatgcgcca caaggattgt
    19681 cagaatccgc tgcgtgtcta ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc
    19741 atgaacacca acggagcagg ggatggcgca ctggcagcgt tgctgcatga cattaccgcc
    19801 aacagctacc atcgtagcaa cgtaccaaac tccagcaaac ataaattcac ctggttaact
    19861 tattcatcgt tagcgcaggt gtgtaaatat gctaaccgtg tgagctatca ggtactgaac
    19921 cagcattcac ctcgtttaac gcgcggcttg ccggagcgtg aagacagcct ggaagagtct
    19981 tactgggatc gttaagttat cgtcggttcg taggccagat aaggcgttca cgccgcatct
    20041 ggcattttgc tctcaatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacatatttt
    20101 tcatatttta catccggcaa ccaccgttta ccccgtcacc acttcacccg ccggtggcgt
    20161 ttccagcagt tccagcatag tacgggcaat ttcacgctcg cccatcacta cttgattcgc
    20221 accacgttcg gtgatatacg ccacttcatc gtcataatgg gcgcgggcaa taatctcaat
    20281 atccggattt ttcgcgcggg cagatgccac aatctcaccc gcttcataac cgttggggat
    20341 cgtcaggatc agccattttg cacattccag atgcgccagt tgcataattt cttcgttcgc
    20401 cgcattgccc aatactgcgc ggaccccgcg ctctcgcagc tcatcaacac gggttcgtga
    20461 cgtctcaatc accaccagcg gaatatcaga ggcgagcaat ttctccccca gcaggctgcc
    20521 tacacgaccg taacccacca atagcgcatg gttgcaaata tccactggga tctgcttctc
    20581 ttcttcgatt gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt
    20641 ctccagtagt gcgaacagta ctgggttgag cataatcgac aggatcgccc ctgccagtac
    20701 caggttttgt ccggcctgcg gcagtaaatt caatgccatt cccagtcccg ccaggataaa
    20761 cgcgaactca ccaatctgcg ccaggctggc ggcgatagtt aatgccgtac gttgggagtg
    20821 accaaacagt cgcaccagga aaaacgcggc taacgactta ccaaacagaa taatcgccag
    20881 cgtcgccagc actgccagcg gttgctgaat cagaattaac ggatcaaaca acatcccgac
    20941 ggagacaaaa aacagcaccg caaacgcgtc gcgcaatggc agcgtatcgt gggcggcacg
    21001 gtgactcagt tcagactcgt tcagtaccat cccggcaaag aacgcaccga gtgcaaagga
    21061 gacatcaaac agctctaccg caccaaaggc aatccctaac gccagcgcca gcaccgacag
    21121 ggtaaacagc tcgcgagaac cggttgccgc gctgcgtgcc ataatccacg gcaccagacg
    21181 gcgacctacc agcatcataa tggcgataaa cgcgatcact ttgccgatgg tgatccccat
    21241 atcgactgca agagtggcaa agcccacatc gccctgttcc atcattcctg ccactgcggg
    21301 cagcaacacc agcgtcagaa ccattaccag gtcttccaca atcaaccaac cgatggcgat
    21361 ttgcccacgc tgactgtcaa ttaattgccg ttcttcaagt gcgcgcagta acaccacggt
    21421 actggcggtg gaaagacata aaccgaacac gataccggtc attaacgacc agcccagcac
    21481 ggcagagagc gccataccca gcagcgtcgc cacggctatc tgggcgatcg caccgggaat
    21541 ggcgatggcc tttaccgcca tcaaatcctt gagcgaaaag tgcagaccca cgccaaacat
    21601 caacagaatg acgcccagtt cagccagttc cggcgcaagc ttggtatcgg caacaaagcc
    21661 cggagtgaat ggtcctgcca gcacacccgc taacagatat cccaccagag gagaaatacg
    21721 tagtttattg gccagcatgc cgaggataaa ggcgagcaca aggccgccaa caatggtggt
    21781 gataagcggg gtggcgtgat gcattccgtc tccttttcct ggtggttatt gtccattttt
    21841 ggccgggaaa accaaaatta caggtaatag tttatgacaa tttcattgat gatgttcatg
    21901 aataattatt gaattttgca gaaaaatgga attagctgca aaaaagcacg gatcggaaaa
    21961 cagaaggcgg tttaacagag gaaaggctta tggcgactgt aaaacgatgc agccaaagtt
    22021 tgcctttggc tgcaatgaaa tcagtcttta tgccggttat caggcaggaa tatggtcaac
    22081 atccccaata gtggcaggaa agcacatatt ttatagacta actcgatgct ggtgtgatcg
    22141 gcgataagcc ccagaactgc cgctcccaga cctcccatgc cgaaagcaaa accgaaaaag
    22201 agtccagaaa ccataccgat acgtcctgga agcagctcct gagcgtagac cagaatggca
    22261 gagaatgccg aagcgaggat aaatccaata atcaccgtta aaacccccgt ccagtgcagg
    22321 ctggcgtagg gtaaaatcag cgtaaacggc gcaacgccga ggatagagcc ccaaatcaca
    22381 tatttccgcc caattttatc ccctacaggc ccgccgatca ccgtacctgc cgcaacggca
    22441 aacaggaagg caaacagatg aagctgagca ttctggatag ataatccgaa tttttgcatc
    22501 agataaaagg tgtaatagct gctgatgctc gccatataga aatatttcga gaaaatgagg
    22561 attaacagaa tgctgaccgc cagtaccact ttgttgcgcg gcagtggatt gataatcgtc
    22621 gctttgggtt ttcctttatt cattcggtgc tgtgccgagt accaacggct gatttgcgcc
    22681 aacaccacga tcgccagcag tgccgcaagc acaaaccagg caacgttgcc tttgccataa
    22741 ggcgcgataa tcaccgccgc cagcaagggt cccagggaac tgccaaagtt gccgccgacc
    22801 tgaaagatag attgtgccag gccatgccgc ccgccggaag ccatacgggc cacgcgagaa
    22861 gattccggat gaaagaccga tgaaccggta ccgaccagcg ccgccgccag cagaactgcg
    22921 ccaaaactgc ccgccagcgc aagcagcacc agaccactta aggtaaagca catgccaatt
    22981 ggcaacgacc acggcatcgg atatttatcg gtccagtagc cgaccactgg ttgcagtagc
    23041 gaagaggcga gctggaaggt gagggttatc atgccaatct gcataaatgt cagagaaaat
    23101 tctgactgaa gcagaggata aatcgccaga atcagcgatt ggatcatgtc gttcagcaga
    23161 tgtgagaggc tgatagcacc taaaatacca aacgatgttc gggccttggt cgttgacgca
    23221 gccgcgcccg ccacaggctg ggtttgttca ctcattgcca taggaaagtc actttttcag
    23281 ggttgcgatg ttaagaatga tcttatttgt gattattacc cgactaacat acctgtatgc
    23341 gccgcctgaa ggaagtctca acgctgaata cagaatttct aatctggatg cagatttatc
    23401 ttcaccggac gcagacttgt ctatgatgtc gcgtcatact atttttcaac acgttgaaat
    23461 caggtcaggg agagaagtat gaaattattg cagcggggcg tggcgttagc actgttaacc
    23521 acatttacac tggcgagtga agctgctctg gcgtatgagc aggataaaac ctacaaaatt
    23581 acagttctgc ataccaatga tcatcatggg catttttggc gcaatgaata tggcgaatat
    23641 ggtctggcgg cgcaaaaaac gctggtggat ggtatccgca aagaggttgc ggctgaaggc
    23701 ggtagcgtgc tgctactttc cggtggcgac attaacactg gcgtgcccga gtctgactta
    23761 caggatgccg aacctgattt tcgcggtatg aatctggtgg gctatgacgc gatggcgatc
    23821 ggtaatcatg aatttgataa cccgctcacc gtattacgcc agcaggaaaa gtgggcaaag
    23881 ttcccgttac tttctgccaa tatctaccag aaaagtaccg gcgagcgcct gtttaaacca
    23941 tgggcgctgt ttaagcgtca ggatctgaaa attgccgtta ttggtctgac gacggatgac
    24001 acagcgaagt taggcaatcc agaaaacttc accgatattg agttccgtaa gcccgccgat
    24061 gaagcgaagc tggtgattca ggagctgcaa cagacagaaa agccagacat tattatcgcg
    24121 gcgacacata tggggcatta cgataacggc gatcacggtt ctaacgcacc gggcgatgtg
    24181 gagatggcac gcgcgttgcc tgccggatcg ctggcgatga ttgtcggcgg tcactcgcaa
    24241 gatccagtct gtatggcagc ggaaaataaa aagcaggtag attacgttcc aggtacgcca
    24301 tgcaaaccgg atcaacaaaa cggcatctgg attgtacagg cgcatgagtg gggcaagtac
    24361 gttggtcggg ccgactttga gtttcgcaac ggcgaaatga aaatggttaa ctaccagctg
    24421 attccggtga acctgaagaa gaaagtgacc tgggaagacg ggaaaagcga gcgcgtgctg
    24481 tacacccctg aaatcgctga aaaccagcaa atgatctcgc tgttatcgcc gttccagaac
    24541 aaaggcaaag cgcagctgga agtgaaaata ggcgaaacca atggtcgtct ggaaggcgat
    24601 cgtgacaaag tgcgttttgt acagaccaat atggggcggt tgattctggc agcacaaatg
    24661 gatcgcactg gtgccgactt tgcggtgatg agcggaggcg gaattcgtga ttctatcgaa
    24721 gcaggcgata tcagctataa aaacgtgctg aaagtgcagc cattcggcaa tgtggtggtg
    24781 tatgccgaca tgaccggtaa agaggtgatt gattacctga ccgccgtcgc gcagatgaag
    24841 ccagattcag gtgcctaccc gcaatttgcc aacgttagct ttgtggcgaa agacggcaaa
    24901 ctgaacgacc ttaaaatcaa aggcgaaccg gtcgatccag cgaaaactta ccgtatggcg
    24961 acattaaact tcaatgccac cggcggtgat ggatatccgc gccttgataa caaaccgggc
    25021 tatgtgaata ccggctttat tgatgccgaa gtgctgaaag cgtatatcca gaaaagctcg
    25081 ccgctggatg tgagtgttta tgaaccgaaa ggtgaggtga gctggcagta atccgaaagt
    25141 gccggatgtt cgcatccggc acaatgctta atcgcggcgg gcgatatcag caaatttggc
    25201 atcgaggatc ttcgccagat cgcctgccgc cagttcgata tccagtccgc gcttgccgcc
    25261 ggaaacataa atagtggcaa attcttgtgc gggggcgtcg ataatcgttg gcagacgttt
    25321 tttctgcccc agtgggctaa tccccccaac caggtatccc gtcgaacgct gcgcgaccat
    25381 cggatcggcc atctcaactt tcttggcacc cagcgctttt gctacttttt taaggtccag
    25441 ttgacctgcg accggcgtaa cggccacggc aaggtgtttc atatcaccgt tcactgcgac
    25501 cagcagcgtt ttgtagacct gatccggatt caaacctaat tttttgacga cttcatcgcc
    25561 aaaattggtt tcagccggat cgtgctcgta ggtatggatt tgaaacgaaa tcttgttttt
    25621 ttcgagtgat ttaactgcgg gtgtcatggt aattttcctg ccttaaacaa aaaaacgaag
    25681 caagcatacg cctgatggcg gtccaaaaaa aagagtcatc ttgcctaaga gtattggcag
    25741 gatggtgaga ttgagcgaca atcgagttac accgtcgctt aaagtgacgg cataataata
    25801 aaaaaatgaa attcctcttt gacgggccaa tagcgatatt ggccattttt ttagcgcaac
    25861 atttgcggta aattcccttc tccatacagg tgtagtgcac cgaccgcgac cacatatcgc
    25921 cccggcggca tggcgcgtaa tttatcccgc caggcgagat ttcgctgatg catcagcaca
    25981 tcgtacagcg actgactgaa cgtattgggc agcgttattt cattattttg cggcggcgca
    26041 ttcagccacc agctcatcat ttgttgcagc aaccgtgcgt tggtatgcca gtgggtcagc
    26101 gtatcgtcca gcagcgccag tcctttgtca gggagctgga gcaacatggc aatctggttt
    26161 tccgcccctt ccagttcaat cacgggttta tgttgttgct tcgccgcctg caatagctga
    26221 taatcgatac cgtattctgc ccgcagcccc agtttttgtg cctgcgtcgc ctgaagaacc
    26281 atcgcgattt gccacagcgg ttgggtagaa aagagtgagg gagaaatgcc catctcctgg
    26341 ctaatgtgct gcagtttttg gagttgctcc tcgctaatgc gctcttccag cgcctcgcag
    26401 gcaggcaaat tagcaaaagg cgtatcgctg gtggaaacat ccgcctcgac gatcagcgca
    26461 tcggcgtttt tgagcttttt gagcaaacgg gtgggtaggg gagccatatc gtggctaccc
    26521 atatgaatac tgccaatcag atgaaaatgg cgattgccgg gaagagagat atcaatggca
    26581 ggccaggtgt aatgattacc gcgcagcgcg gcccaaagcg tttttacccg gtacaacaga
    26641 tccatacgac ctccctttgt gaaatatcat gctagcgcgc ggggagggat ggcgcaaatg
    26701 gagttgggga cttgtttaat gtgtttgtat gattcagtat gttcttgtat cgctatttac
    26761 agggaagcaa cagttaaaaa ctatgaaaca ggcaacaaga aaaccgacga cacccggaga
    26821 tattcttctc tatgaatatc tggaaccgct agatttgaaa atcaatgagt tagcagagtt
    26881 gctgcatgtt catcgtaata gcgtcagtgc actgatcaat aacaatcgta aactcaccac
    26941 tgagatggca ttccgtctgg cgaaagtttt tgataccaca gtcgattttt ggctaaacct
    27001 ccaggcggca gttgatcttt gggaagttga aaacaacatg cgcacccagg aagaattggg
    27061 acggattgaa acagtggctg aatatttggc acgccgtgaa gagcgtgcaa aaaaggtcgc
    27121 gtaaggccaa aacatcgctt catctcgtag gccggataag gcgttcacgc cgcatccggc
    27181 atccgagcac cattgcctga tgcgacgctt acgcgtctta tcaggcctac aaacctgtgc
    27241 ctgaaccgta ggccggataa ggcgtttacg ccgcatccgg cagtcatgcg tcgatgccaa
    27301 atgcgccacc ctaaagcagc gcatccgcaa tgacgtactt attccttcgg tttaaaccgc
    27361 agcaaccggt tggcgttact cactacggta atcgacgaga gcgccattgc cgctccggca
    27421 actaccgggt taagcagtgt tccagtaaac ggccacaaaa taccggcggc gaccggaata
    27481 ccgatactgt tgtagataaa cgcgccgagc aggttctgct tcatgttgcg cagcgttgcg
    27541 cgggaaatag cgagcgcatc cgcaacgccc atcaggctat ggcgcatcag ggtaatcgcc
    27601 gcggtttcaa tggcaacatc actgccgccg cccattgcaa tgccgacatc cgcctgggcc
    27661 agcgctggcg catcgttaat gccgtcgccc accattgcca cctgacgtcc ttcactttgc
    27721 aggcgtttaa tcgcttcggc tttaccgtcc ggcagcaccc cggcgatcac ctcatcaatc
    27781 cctgcttctt tggcgatcgc attggcggtg gttgggttat ccccggtcaa catcaccaga
    27841 cgatatcccg ctttatgcag acgttgcagc gccgccacgc tatcactacg caacggatcg
    27901 cgtacagcca gcagggctac cgctttcccg tcaaccgcca gcagcacagg cgttgcccct
    27961 tgcgatgcct gagctgaaat atctgcttca atcgctttag tatcaacttg ctggtcattt
    28021 aacagcgcct gattgcccag caataacgca tgaccttcag cttcaccgct cacgcccagc
    28081 ccgcgcaatg tgcggaaacc gttgacctgc ggcagctgca tatcgcttgc tttatcgagg
    28141 atcgcccgcg ccagcggatg gctggaacct tgttccagtg ccgccgccag acgcagcgcc
    28201 tgcgcttcgt caaaatcagc aaatgttttc actgcgacaa cctgcggctt cccttcggtc
    28261 agcgtcccgg ttttatcgaa cactacggta tcgagtgtac tagcgcgttg cagcgcgtcg
    28321 gcgtcccgca ccagcacgcc aaactcagcc gcccgcccga cgccggaaat aatcgacatc
    28381 ggcgtcgcca gccccagcgc acacggacag gcaataatca gcaccgtggt ggcaatcacc
    28441 agggtataga caatctgcgg tgccggacca aagaaatacc agattgccgc actgacaagc
    28501 gcaataacca ctactaccgg cacaaatacg gctgagattt tatccgccag ctgaccgatt
    28561 tctggcttgc tgctctgggc ctggcgcacc atacgaataa tccttgatag cgtggtatgg
    28621 ctgccaaccg cactggcgcg aaacagaacg ctaccgtcct gtaccactgt cccggcatgg
    28681 acgctatcgc cttcgccttt ttgctgcggg ataggttcgc ccgtcagcat cgcttcatcc
    28741 agccatgctt cgccctgggt aatctcgcca tctactggca cgcggtcgcc cgtcgtcagg
    28801 cgcagtagca tgcctggctg aacttccgcc agcggcacgc ttttttcacc ttcgtcagta
    28861 accaggcgcg ccgtcggcgg agttaaatcg agtaatttct ccagcgcctt tgaagaacgc
    28921 tgacgtgcgc gcgcttccag catatgaccg agattaatca gaccgataat catcgcactg
    28981 gcttcatagt aaagatgccg cgcttccatc gggaaccact gcggccacag gttgacgctc
    29041 atcgaataga gccacgccac acccgtaccc agcgccacca gcgtatccat cgtcgccgca
    29101 ccgttcagca ggcttttcca tgcactgcgg taaaaatggc cgccggcgaa aaccatcact
    29161 gccagggtta tcaggccgat aaccaaccac aggctgcggt tgtcagcggt gaccatcatg
    29221 ttatcgccga tcatccccca gaccattacc gggataccca cagccagtgc aacaattgcc
    29281 tgccagcgga agcgcttcat cgtagcgacg gcggtttctt gctggcgctc gcggcgtttc
    29341 gcgtcatctt caatcgcttc cgcgccgtag cccgcttttt ccaccgcctg cactaaatct
    29401 tgtggggagg cactgcccat caccagcgca gtacgctccg ccaggtttac ccgtgcctga
    29461 gtgacgcccg gtacgctttg cagcgcattt tgtacgcggg tgacacagct ggcgcagctc
    29521 atgccgctca gcagcaactg ctggctgtca tcgtcatcgg cggtcgctgc cggaagcgcc
    29581 tcagaaaccg ctgtcagtgc ttccgacggg attgatgact ccgccagcgg tttagccttt
    29641 gggtggctta cagatgcgtc ataacccgct tgtttgatgg tttcgatcag ctgttctgca
    29701 ctggcagtcc cggtaacgtg cgcttcagtg atagacacat ccgcttgttc cacatccggg
    29761 cgctgttcga gactttcttt cacgcgttta acgcagtgac cgcaggacag gccgtccagg
    29821 gtcaggtcga tagtttgtga cataaaacac tcctttaaga cagttttgac tggctgtgat
    29881 aaaggttaaa ccttccagca aggggaaggt caagaaatta ataacccagg cgagtaaaag
    29941 tccgtaaaga ttaaaaaatc ggctcgattt gcatcaggat tagacattta tctctttgtt
    30001 ttcctgtagt taagttgcgg gtgctaagtt aaatcaggat gcctgaaaat cggcaccggg
    30061 gtgaggaatt acctcccgca tctataaaaa ggagttaaca aaagatgtta gatgcaaaca
    30121 aattacagca ggcagtggat caggcttaca cccaatttca ctcacttaac ggcggacaaa
    30181 atgccgatta cattcccttt ctggcgaatg tgcctggtca actggcagca gtggctatcg
    30241 tgaccagcga tggcaacgtc tatagtgcgg gtgacagtga ttaccgcttt gcgctggaat
    30301 ccatctcgaa agtctgtacg ttagcccttg cgttagaaga tgtcggcccg caggcggtac
    30361 aggacaaaat tggcgctgac ccgaccggat tgccctttaa ctcagttatc gccttagagt
    30421 tgcatggcgg caaaccgctg tcgccactgg taaatgctgg cgctattgcc accaccagcc
    30481 tgattaacgc tgaaaatgcc gaacaacgct ggcagcgaat tttacatatc caacagcaac
    30541 tggctggtga gctggtagcg ctctctgacg aagtcaacca gtcggaacaa acaaccaact
    30601 tccataaccg ggccattgcc tggctgctgt actccgccgg atatctctat tgtgatgcaa
    30661 tggaagcctg tgacgtgtac acccgtcagt gctccacgct catcaatact gttgaactgg
    30721 caacgcttgg cgcgacgctg gcggcgggtg gtgtgaatcc gttgacgcat aaacgcgttc
    30781 ttcaggccga caacgtgccg tacattctgg ccgaaatgat gatggaaggg ctgtatggtc
    30841 gctccggtga ctgggcgtat cgtgttggtt taccgggcaa aagcggtgta ggtggcggta
    30901 ttctggcggt cgtccctgga gtgatgggaa ttgccgcgtt ctcaccaccg ctggacgaag
    30961 aaggcaacag tgttcgcggt caaaaaatgg tggcatcggt cgctaagcaa ctcggctata
    31021 acgtgtttaa gggctgatca tgatgaacac ggaaggtaat aacggtaaca aacccctcgg
    31081 tctatggaac gtcgtttcca tcggcattgg ggcaatggtg ggggcgggga tcttcgcgct
    31141 gctggggcag gctgcgttgc taatggaagc ctcgacctgg gtcgcctttg cttttggcgg
    31201 tattgtggcg atgttttccg gttatgccta tgcgcgtctg ggggcgagct atcccagcaa
    31261 tggcggcatt atcgacttct ttcgtcgcgg attaggcaac ggcgtctttt cgctggcgct
    31321 ctcgttactg tacctgttga cgctggcggt gagcatcgcc atggtcgccc gtgcttttgg
    31381 cgcttatgcc gtgcagtttt tgcatgaagg cagccaggag gagcacctta ttttgctcta
    31441 cgcgttgggg atcattgcgg tgatgacgct tttcaactcc ttaagcaacc atgcggtagg
    31501 gcggctggag gtgatcctcg tcggcattaa aatgatgatc ctgttattgc tgattattgc
    31561 cggtgtctgg tcgctgcaac cggcgcatat ttccgtctct gcgcccccca gctccggtgc
    31621 gttcttctcc tgtatcggga taaccttcct tgcctatgcg ggctttggca tgatggcgaa
    31681 cgcggcggat aaagtgaaag atccgcaggt cattatgcca cgggcgtttc tggtggcaat
    31741 tggcgttacc acgttgcttt atatctcgct ggcgctggtt ttgcttagcg atgtatcggc
    31801 attagagtta gaaaaatatg ccgataccgc cgtagcgcag gctgcttctc cgctgctcgg
    31861 gcatgtgggt tatgtgatcg tcgtcatcgg cgctttactg gcgacggctt cagccatcaa
    31921 cgcgaacctg ttcgccgtgt ttaacatcat ggacaacatg ggcagcgaac gcgaactgcc
    31981 gaagcgaatg aataaatccc tgtggcggca gagtacctgg ggcaacatca ttgtcgtggt
    32041 gttgattatg ctgatgacgg cggcactaaa tttaggctca ctcgccagcg ttgccagcgc
    32101 cacctttttg atttgctatc tggcggtgtt tgtggtggcg atccgcctgc gtcatgatat
    32161 tcacgcctcg ttgccgattc ttatcgtagg tacgttggtg atgttgttgg tgatcgttgg
    32221 ctttatctac agcctgtggt cccagggcag ccgtgcgttg atatggatta ttggtgcaat
    32281 cttactcagc cttattgtgg caatggtcat gaagcgcaat aaaaccgtat aacacctctc
    32341 tgcgcgcagt actgcctgta ctgcgccttt gtctttaatt caaattacct ttcttattcc
    32401 tttaataaat aagtcacaaa aaaatgatat atacgcgata aatctgtgat gctaatcact
    32461 tattaccgtg gattattttt aatgtgtatg atggtaatct aaaagtcgta gtgtattcta
    32521 aataaataac
//
LOCUS       DAZKIF010000045        32297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZKIF010000045 DAZKIF010000000
VERSION     DAZKIF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.045"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(170..1033)
                     /gene="potB"
                     /locus_tag="ACHOTW_002069"
     CDS             complement(170..1033)
                     /gene="potB"
                     /locus_tag="ACHOTW_002069"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP9844179.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(1017..2153)
                     /gene="potA"
                     /locus_tag="ACHOTW_002070"
     CDS             complement(1017..2153)
                     /gene="potA"
                     /locus_tag="ACHOTW_002070"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP9844180.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2403..3629
                     /gene="pepT"
                     /locus_tag="ACHOTW_002071"
     CDS             2403..3629
                     /gene="pepT"
                     /locus_tag="ACHOTW_002071"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9844181.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(3678..4799)
                     /gene="roxA"
                     /locus_tag="ACHOTW_002072"
                     /gene_synonym="ycfD"
     CDS             complement(3678..4799)
                     /gene="roxA"
                     /locus_tag="ACHOTW_002072"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP9844182.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            5048..6277
                     /locus_tag="ACHOTW_002073"
     CDS             5048..6277
                     /locus_tag="ACHOTW_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9844183.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            6642..6830
                     /locus_tag="ACHOTW_002074"
     CDS             6642..6830
                     /locus_tag="ACHOTW_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9844184.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            6888..7631
                     /locus_tag="ACHOTW_002075"
     CDS             6888..7631
                     /locus_tag="ACHOTW_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844185.1"
                     /translation="MKIEYTPERGRGFVRPGETEKQQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHTCNERFSLYSLRNCSRAKAHGAN
                     LSDSCSIFLRRLFRAGDNVLVNSLSVIVLSCIAMRRNTSHHGADSFYPLGSIPRRFSS
                     VRPNSLSQLARLMPSLCASLSNCSFSSGEIRILNCGDCPSPLGLLSRLIVDKWSPIEL
                     AFLLLGGHLNTRALKKAKPRKCHYHSQGF"
     gene            7657..7854
                     /locus_tag="ACHOTW_002076"
     CDS             7657..7854
                     /locus_tag="ACHOTW_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9844186.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGVCHA"
     gene            7847..8032
                     /locus_tag="ACHOTW_002077"
     CDS             7847..8032
                     /locus_tag="ACHOTW_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844187.1"
                     /translation="MLKTFRVFASAVNPLGHTIGIAQNVKAVNVQTAIAAVRSESSEY
                     GLSQVIISAVYELKEVH"
     gene            8032..8223
                     /locus_tag="ACHOTW_002078"
     CDS             8032..8223
                     /locus_tag="ACHOTW_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844188.1"
                     /translation="MKEITLHEAAERAHQTEIICRLLEVYPDKLNDGDISALAGLLAK
                     LSGSVALWLIDERAERYEK"
     gene            8213..8455
                     /locus_tag="ACHOTW_002079"
     CDS             8213..8455
                     /locus_tag="ACHOTW_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844189.1"
                     /translation="MKNNKLATSELRSLANSAISLNNAANEVMNMWLQSLVNNERDES
                     TARLILAVSELLDSSTSKIESISEKINGLSGHAIGE"
     gene            8461..8760
                     /locus_tag="ACHOTW_002080"
     CDS             8461..8760
                     /locus_tag="ACHOTW_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9844190.1"
                     /translation="MKLKYPGLTDSGKTRTKFMRGDIYRDQYGGTVMIKGVEERRVTY
                     HREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            8757..10889
                     /locus_tag="ACHOTW_002081"
     CDS             8757..10889
                     /locus_tag="ACHOTW_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFP9844191.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEQDGPAS
                     GDNVPPVWLGEQQLSELDKLQIVPEGRKSVRIFRAGHLAPVMIKAIGQKLAAAGVQDA
                     NFYPEGMHGQKVENWREYLARERQNLSDGLVIEFPVKKKDTGSHSDDELKPRVESRAD
                     GVFWVTPKVDKQSGEIIRPETWLCSPLELLGTGTIGKEHYRVMRWKKTANHEVITMAI
                     PCGGIGDRDGWRLLKDHGLNVTTNGKYRAILADWMQLSGSHEEWQLSTTTGWHFGAYI
                     MPDGSIIGESEKPILFTGKSAAINGYSVAGTADGWRDSVARLAGGNASMILGVATSLA
                     APLIGLVGADGFGVHLFEQSSAGKTTTQNIASSLWGEPDAQRLTWYGTALGIANEAEA
                     HNDGLLPLDEIGQAGNAREVSTSAYTLFNGSGKLQGAKDGGNREIKHWRTVAISTGEM
                     DVETFLKSEGIKVKAGQLVRLLNVPMEKATKFHEYSNGKEHADALKDAWTANHGAAGR
                     EWVKWLAGHQQEAKDTVRECRERWRNLIPESYGEQVHRVGERFAILEAALVLSGHVTG
                     WVVQECRDAIQHNFNAWVKEFGTGNREFKQMVEQAEAFLSSFGFSRYLPHPNTDERDL
                     PIKELAGYRKGSIRNEDDEMRYYTFPHVFESEIAKGFNPAHFARALDAAGMLEKGSDR
                     RYKKKALGKIGGKQHVFYVLMFQPDDED"
     gene            11260..11511
                     /locus_tag="ACHOTW_002082"
     CDS             11260..11511
                     /locus_tag="ACHOTW_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844192.1"
                     /translation="MTRTRRDRTEPKYKALDITELALKVAIRTIDRHVGEGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            11508..11918
                     /locus_tag="ACHOTW_002083"
     CDS             11508..11918
                     /locus_tag="ACHOTW_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9844193.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDGIPF"
     gene            11929..12201
                     /locus_tag="ACHOTW_002084"
     CDS             11929..12201
                     /locus_tag="ACHOTW_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9844194.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQKPPEPPDNFGDLRKAVDRTYAEMGIDGVSDEIWRNYPDS"
     gene            12326..12550
                     /locus_tag="ACHOTW_002085"
     CDS             12326..12550
                     /locus_tag="ACHOTW_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844195.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            12843..14000
                     /locus_tag="ACHOTW_002086"
     CDS             12843..14000
                     /locus_tag="ACHOTW_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9844196.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLTDESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKTREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            14040..14612
                     /locus_tag="ACHOTW_002087"
     CDS             14040..14612
                     /locus_tag="ACHOTW_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFP9844197.1"
                     /translation="MGEKIMKSMEIRSSEITTSASNTLTGYVVRWDNLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            14614..15825
                     /locus_tag="ACHOTW_002088"
     CDS             14614..15825
                     /locus_tag="ACHOTW_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9844198.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MVRHCLLNGNAYAVIQWGRDGFPVALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTHN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            15822..16160
                     /locus_tag="ACHOTW_002089"
     CDS             15822..16160
                     /locus_tag="ACHOTW_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9844199.1"
                     /translation="MNIGRLRDRITIQTLKQTRAMTGEILETWEDGHTLWASVNMISS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDVARTRL
                     EILCREGAEK"
     gene            16157..16453
                     /locus_tag="ACHOTW_002090"
     CDS             16157..16453
                     /locus_tag="ACHOTW_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9844200.1"
                     /translation="MTEELITLEEVKLHCRIDGDEEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            16453..16893
                     /locus_tag="ACHOTW_002091"
     CDS             16453..16893
                     /locus_tag="ACHOTW_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9844201.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPIDGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            16877..17059
                     /locus_tag="ACHOTW_002092"
     CDS             16877..17059
                     /locus_tag="ACHOTW_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844202.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            17183..17539
                     /locus_tag="ACHOTW_002093"
     CDS             17183..17539
                     /locus_tag="ACHOTW_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFP9844203.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            17523..19184
                     /locus_tag="ACHOTW_002094"
     CDS             17523..19184
                     /locus_tag="ACHOTW_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9844204.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNESMFVLIYELDDESEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFHIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            19198..19479
                     /locus_tag="ACHOTW_002095"
     CDS             19198..19479
                     /locus_tag="ACHOTW_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844205.1"
                     /translation="MTEAELLGLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMC
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVELHREKEKITYHWKKPERLRR"
     gene            complement(20336..21796)
                     /gene="phoQ"
                     /locus_tag="ACHOTW_002096"
     CDS             complement(20336..21796)
                     /gene="phoQ"
                     /locus_tag="ACHOTW_002096"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP9844206.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(21796..22467)
                     /gene="phoP"
                     /locus_tag="ACHOTW_002097"
     CDS             complement(21796..22467)
                     /gene="phoP"
                     /locus_tag="ACHOTW_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP9844207.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(22636..24006)
                     /gene="purB"
                     /locus_tag="ACHOTW_002098"
     CDS             complement(22636..24006)
                     /gene="purB"
                     /locus_tag="ACHOTW_002098"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9844208.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(24010..24651)
                     /gene="hflD"
                     /locus_tag="ACHOTW_002099"
     CDS             complement(24010..24651)
                     /gene="hflD"
                     /locus_tag="ACHOTW_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP9844209.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(24687..25793)
                     /gene="mnmA"
                     /locus_tag="ACHOTW_002100"
                     /gene_synonym="trmU"
     CDS             complement(24687..25793)
                     /gene="mnmA"
                     /locus_tag="ACHOTW_002100"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9844210.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            complement(25847..26308)
                     /gene="nudJ"
                     /locus_tag="ACHOTW_002101"
     CDS             complement(25847..26308)
                     /gene="nudJ"
                     /locus_tag="ACHOTW_002101"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP9844211.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(26318..26971)
                     /gene="rluE"
                     /locus_tag="ACHOTW_002102"
     CDS             complement(26318..26971)
                     /gene="rluE"
                     /locus_tag="ACHOTW_002102"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP9844212.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            27143..28393
                     /gene="icd"
                     /locus_tag="ACHOTW_002103"
     CDS             27143..28393
                     /gene="icd"
                     /locus_tag="ACHOTW_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP9844213.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(28507..29649)
                     /locus_tag="ACHOTW_002104"
     CDS             complement(28507..29649)
                     /locus_tag="ACHOTW_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9844214.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(29639..29875)
                     /locus_tag="ACHOTW_002105"
     CDS             complement(29639..29875)
                     /locus_tag="ACHOTW_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9844215.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            30015..30803
                     /locus_tag="ACHOTW_002106"
                     /pseudo
     CDS             30015..30803
                     /locus_tag="ACHOTW_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            30800..31501
                     /locus_tag="ACHOTW_002107"
     CDS             30800..31501
                     /locus_tag="ACHOTW_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9844216.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            31498..31800
                     /locus_tag="ACHOTW_002108"
     CDS             31498..31800
                     /locus_tag="ACHOTW_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP9844217.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            31868..32200
                     /gene="emrE"
                     /locus_tag="ACHOTW_002109"
     CDS             31868..32200
                     /gene="emrE"
                     /locus_tag="ACHOTW_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP9844218.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAVIGMMLICAGVLVIN
                     LLSRSTPH"
BASE COUNT         8416 a         7671 c         8547 g         7663 t
ORIGIN      
        1 tcatttttat accatttgtt gtttttttat agcgatttgc taaaagggag aagaaaaaac
       61 gttctccaaa attagattgc aactgtttga ttttatggat agaggcatgc tgtttactag
      121 acagtaaaaa gcatgccaaa tttcctatta accttttgaa tataaagcac taattatcta
      181 tttctgttgc cttcttattc agcagacgag aagcgcgcca gtaaaccagc aacatcaggc
      241 ccattacgat agtcagcgta atgctggtgg ctgcaccaaa cggccagtca cgaatattga
      301 ggaactgtac cttgatgacg ttaccgatca gcaggttttt cgcaccgccc atcaggtcgg
      361 acacatagaa caggcccatc gctggcagca tcaccagcag acatccggca ataatccccg
      421 gcatcgtcag tggaataatg atgcggataa aagtctgtaa cttgctggca ccgagatcgc
      481 gcgccgcctc aagcaacggc ttatccagtt tttcgatact ggagtacagc ggcatcacca
      541 taaacggtag cagaatgtaa accagaccga taatcaccgc actgggcgtg aacatgatac
      601 gaattggtgt gtcgataacg cccagccaga gcaaaaactc gttgagatag cctttggtgc
      661 tgaggaaaat tttcagcccg tagatgcgaa tcagtgagtt ggtccagaag ggaacaatca
      721 gcagaaacag cagcagcgga cgcaccttgt gtggcagctt cgccagaaac caggcaaacg
      781 gatagcccag caccaggcag gcgagggtgg cgatcagcgc catattcagc gagtgcaata
      841 gcacttcaaa atagagcgga tcgagcagac gtgtgtagtt atccagcgta aagaccattt
      901 tgacaaaact ggcgtcgtcg cgggccaaaa agctggtgcc aatgatcatc aggttgggca
      961 gaaagacaaa caacacaagc caaccgacaa tagtgacaat cactacattc tggaacttac
     1021 ttgtgttctt catcagccag tacgacctcc cagctttcta cccaattaat ggccattttt
     1081 tggtcgagag agtggtcaaa gtcaggatcg tcttcattga agaattcgct gaccatcacc
     1141 atcttgccat tttccagttc aacaaccgac tccagcgtca tgcctttgta gttacgctcg
     1201 cgaacgtaac caatcagccc ttcagcgtgg ttgtcgtcgt taatctcttc aacacgtaag
     1261 tcttccgggc gcagcagaac atgcagtttt tgccccggtt caacggcgaa gttaacgtag
     1321 atattacatt cgcggccttc aacgttggcg cgtacgcgct gctcgtcgag acgttcgatg
     1381 acagtggcgt taaacatatt gatttcgcca atgaacccgg caacaaacag gtttttcggc
     1441 tcttcgtaga tttcacgcgg cgtgccgtct tgctcaatgc gaccatcgcg catcaccaca
     1501 atcctgtctg acatggtgag tgcttcttcc tgatcgtgag tcacgaagac gaatgtaatg
     1561 ccaagcttac gctgtaacgc tttcagctcg ttctgcattt gcttacgcag tttgtaatcc
     1621 agcgctgaga gcgactcatc cagcaacaac agacgaggct tgttaaccac cgcgcgagca
     1681 atggcgacgc gttgctgttg accaccagag agctgatgcg gtttacgttg agcgaaggtt
     1741 tccagctgca ccatccgcag ggcttccatc acgcggggcg taatttcagc agcgggggtt
     1801 ttttgcatgc gcaacccaaa ggccacattt tcgaacacgg tcatgtgggg gaaaagtgcg
     1861 tagctttgga aaacagtgtt cacatagcgg ttttccgccg gaacgtgggt gatgtcctcg
     1921 ttatccaaca tgatgcgtcc ggaatcaaca gtttccagac ctgcaatcag gcgaagaacg
     1981 gttgttttac cgcagccaga agggccaagc agcgtgagga actcgccatt gttgatagtc
     2041 agatccagct ggggaatgac ctctttacca tcaaagcatt tgcgaattcc cgccaattgc
     2101 accagcggtg aaagcgaact cggttgttta ttcaattttt tactctgtcc catgtaaacg
     2161 caacggatgg cttaccgatg cggggtttgt ggttaaccac cttggtgact cttaatgagg
     2221 gcggtaattc tacggcaaac cgcttgaatc gccaatcttt gttgtgaatt actggcttag
     2281 ctttatattc attaaggtaa tgctgataaa tattcccgct tgcaggggta aaagtgacct
     2341 gacgcaatat ttgtcttttc ttgcttctta ataatgttgt cacaaaaagt gagggtgact
     2401 acatggataa actacttgag cgatttttga actacgtgtc tctggatacc caatcaaaag
     2461 caggggtgag acaggttccc agcacggaag gccaatggaa gttattgcat ctgctgaaag
     2521 agcagctcga agagatgggg cttatcaatg tgaccttaag tgagaagggc actttgatgg
     2581 cgacgttacc ggctaacgtc cctggcgata tcccggcgat tggctttatt tctcatgtgg
     2641 atacctcacc ggattgcagc ggcaaaaatg tgaatccgca aattgttgaa aactatcgcg
     2701 gtggcgatat tgcgctgggt atcggcgatg aagttttatc accggttatg ttcccggtgc
     2761 tgcatcagct actgggtcag acgctgatta ccaccgatgg taaaaccttg ttaggtgccg
     2821 atgacaaagc aggtattgca gaaatcatga ccgcgctggc ggtattgcaa cagaaaaaca
     2881 ttccgcatgg tgatattcgc gtcgccttta ccccggatga agaagtgggc aaaggggcga
     2941 aacattttga tgttgatgcc ttcgatgccc gctgggctta cactgttgat ggtggtggcg
     3001 taggcgaact ggagtttgaa aacttcaacg ccgcatcggt caatatcaaa attgtcggta
     3061 acaatgttca tccgggcacg gcgaaaggag tgatggtaaa tgcgctgtcg ctggcggcac
     3121 gtattcatgc ggaagttccg gcggatgaaa gcccggaaat gacagaaggc tatgaaggtt
     3181 tctatcacct ggcgagcatg aaaggcaccg ttgaacgggc cgatatgcac tacatcatcc
     3241 gtgatttcga ccgtaaacag tttgaagcgc gtaaacgtaa aatgatggag atcgccaaaa
     3301 aagtgggcaa agggttacat cctgattgct acattgaatt ggtgattgaa gacagttact
     3361 acaatatgcg cgagaaagtg gttgagcatc cgcatattct cgatatcgcc cagcaggcga
     3421 tgcgtgactg cgatattgaa ccggaactga aaccgatccg cggcggtacc gacggcgcgc
     3481 agttgtcgtt tatgggatta ccgtgcccga acctgttcac tggcggttac aactatcatg
     3541 gtaagcatga gtttgtgact ctggaaggta tggaaaaagc ggtgcaggtg atcgtccgta
     3601 ttgccgagtt aacggcgcaa cggaagtaag cgaaaaggga tgcggcatgt gatgccgcat
     3661 ccggcttaaa tccaaactta cccttcgaag aaccaatacc cgctattgac cagcgccgcg
     3721 agcatcgcga ggaatgacgg atcttccagc gcatcgccaa aattctccgc agtcagcgca
     3781 atgttgctgg cgagtgcatc cagtgccgga cggtgcgggg aatcgatctt ctcaccattg
     3841 gtatacacgt cgtcgccaat gcgcaatacg cgcagaccac ccaggcgcac cagcacatca
     3901 ccttgtttca gcgcatcgta gatttcatct ggctgataag gcggctccgg cggcgcgata
     3961 tccagttcat gacgtgactg ggatataaac tcgccaaacc attgcttaaa gtgttccggc
     4021 tggttgatca attcgagcat catctcacgc agtttatcca tctcttgcgg cagaacgtcc
     4081 gcaggatgag cgcgaggtgg aacatcagga tcgctgtagt agttgccgcc cagttcgcgt
     4141 tgcagcacat aatcggcaaa tccgctaatt aattcccgcg tatttggcgc gcgaaaaccc
     4201 acggaatagt tcatcgcatt ttccagcgcg tagccttcat gcgggaatcc tggcggaata
     4261 taaagaatat cgccaggctc cagctcttca tcgatgatgg cttcgaacgg atcgacctgt
     4321 aacagatccg gatgtgggca gtgctgtttc atttgcagct tctcgcccac tcgccagcga
     4381 cgacgtccgg taccctgaat gataaacacg tcgtactgat cgagatgcgg gccgacgccg
     4441 ccgccaggta cagaaaaaga gatcatcaga tcatcaatac gccagtccgg tagttcacgg
     4501 aacggtcgca tcagcgcggc ggtcggctca tgccagtggt tcactgcttg cactaacaat
     4561 gaccagttag tttcaccgag atgatcgtag ctttcgaacg gaccgtggct gacctgccat
     4621 tttccatcct ggtgactgac cagtcgactg tcgacttcgc tttccatcgc cagacccgcc
     4681 aactcgtctg gagagatcgg gtcaataaaa ttattaaagc cgcgttttaa caccaccggg
     4741 cgtttctgcc agtgacgttc aagaaaatcg ggccagttaa gagtgagttg gtattccatg
     4801 ttaagcatcc acaggctggt atctgcaacc gattataacg gatgcttaac gtaatgcgtg
     4861 aagtatggac atattttatc aattagtgtt ttttgccgtt tcataatgtt tcagattgtt
     4921 cattatgttt ttgattataa acagatttgt gattttcctt gttccagctt gtttcacgtt
     4981 gtagcccttt gtgcaacgat cggtgtagtt aatggtgtag ttaattgtga tgaccggaat
     5041 caaaattatg agcagagcac ttaacaaact gagcgataca cagctgagga aaatcaacgg
     5101 cacacccgcc caaaaaacag cctttcttaa tgacggtgga aacctgagcg tcaggcattc
     5161 aaccagtggc cttttaacct ggtatttcac ttacagggcc ggaacgggaa ggggggcacc
     5221 accggaacgc attaagctgg gaaattatcc tgatctgagc ctgaaatcag ccagggaaaa
     5281 agccgcccag tgtcgcgcat ggctggcaga ggggaaaaat ccacgtcatg agcttaatta
     5341 caccgtacag gaagcgttaa agccggtaac ggttggcgat gcgctcacct actggcttga
     5401 gtcgtacgca aaggaaaacc gcgtggatta tgccgccctg aaaaagcgcc ttaataatca
     5461 cgtaatacag cacattggtg ctatgccgct ggataaatgc gagctacggc actggctggc
     5521 ctgttttgac caggtggcaa agcgaacgcc tgttactgcc ggattcttgc tacagacgtg
     5581 caaacaggcg cttaagttct gccggaggcg gcgctatgca atcagcaacg ttcttgatga
     5641 tatgagtgtg gcggacgttg ggaaaaaacc ggatataagc gagcgtgtct taagcaccaa
     5701 agaactgggc gaattattgc aggcactgga caaaaaaata ttctccccct actacatcgc
     5761 gttaatccgc ctcctgattg tgttcggatg ccggacggta gaactgaggt tatcggagat
     5821 cagcgagtgg gattttaccg aaatgctctg gaccgtgccg aaagaacaca gcaaaacgaa
     5881 ggtggcaata ttccggccca taccggaagc aatactgccg ttcgtcacgc agctggtgga
     5941 gcagaacagg cacacgggtt tattgctggg ggaagtgaaa caggaaacaa gcgtgtcgca
     6001 gtacggcaga ttagcgcata ggaggctaaa tcaccctcac tggtcactac atgacatccg
     6061 gcgcaccttt acaacgatgc tgaacgattt aggcgtggat cctcacgtcg tggagcagct
     6121 tacaggccac cagatgccag gaatgcagcg agtttataat cattcccgtt atctggatgc
     6181 taaacgcaat gcgctggata tgtggacgga gcggttaggg atactggcgg gaacacatga
     6241 aaacgtaacc acgctaccag tagccagaag aaaataattt ttttcgtgtt ttttcagcat
     6301 gcgcatactg gatatgcgaa cagatacaac gtgcagcaat gagcaacaat gtggaacaac
     6361 cacgaacaag aggcgaaaaa gcgtacggat ttataagtag ctgattttta atgtgttact
     6421 ggttttttat acactagcga atcactcttt aaagagcgag aaaaaaggca tgaaacaata
     6481 ttttatgcct ttgtattaac atgaatttaa aagattttat tcttactgtt tcattttctg
     6541 taaatcatac atcctcagta tttgccattc atcactctag cgggaacaat acgacacaat
     6601 aagacacctg atgactcttt aagaaacgaa agggggcaat agtgttaagc actgatcggt
     6661 ttatacgtga aaaagaatgc gaaaaactaa ccggccttag ccgtacgtgt cgctaccgcc
     6721 tggaaaaggc cggacaattc ccatcacgtc gtaaacttgg cggtcgttcc gttggctggt
     6781 ctttatccga ggttctggcc tggaaggata gctgcaaggc agttcattaa tcacgctggc
     6841 ggcacgcagc cgccacacat caatcatctg aacacagagc tataaccatg aagattgaat
     6901 atacgccaga acgtgggcgg ggattcgttc gccctggtga aactgaaaaa caacaaaatt
     6961 ggggtttttc aggtataaaa aaagcggccc cgaaatggag ccgcctttct gaacagataa
     7021 cccgctgcgc cgtttgtgtg tgtgatccca aacataagca cggggatgat agccgctatc
     7081 aggctggtgg gcaatgcaat cagtctggtt cagttcgttg ccatacctgc aatgagcgct
     7141 tttccctgta ctctttaagg aattgctcaa gggcaaaagc acatggtgcg aatctttctg
     7201 attcatgctc tatctttctg cgccgtcttt tccgtgccgg tgataatgtt ttggtcaatt
     7261 ctttatcggt cattgtgttg tcctgcatag caatgcgccg taatacctca caccacggcg
     7321 ctgatagttt ttatcctttg ggttctatac cgcgccgctt tagttcagta cgccccaact
     7381 ctttaagcca gttggctagg cttatgccgt cgctctgtgc ttctttatcg aattgctctt
     7441 ttagctctgg agaaattcgc attctgaatt gtggggattg cccgtcccct ttagggcttt
     7501 tatcgcgttt gattgttgac aagtggtcac ctattgaatt agcctttcta ttgttaggtg
     7561 gtcaccttaa cacaagagca cttaaaaaag caaagccccg caagtgtcat taccactcgc
     7621 agggcttcta accaacaacg taaactagga gccgttatgg ttgctgtaaa tcatatacca
     7681 caccttgtac acacacaaac ggcctttgtg tggcgttttc tggcactgag tgccggagaa
     7741 tctcaaatca tccacgtaac cgcctggacg gaacgcgaag cgcgtagccg ttgcccgtca
     7801 ggttgtgttg ctgtatttgc cgcccgtatt cgtcaggggg tgtgccatgc ttaaaacctt
     7861 ccgtgtattt gccagcgctg ttaacccact aggccacaca attggtatcg ctcagaatgt
     7921 gaaggctgtt aatgttcaga cggctattgc tgcggtgaga agcgaatcat cagaatatgg
     7981 cttatcacaa gtcattattt cagcagtgta tgaattaaaa gaggtgcatt aatgaaggaa
     8041 attacattac acgaagccgc tgaacgtgcg caccagacag aaattatttg ccgccttctt
     8101 gaggtatacc ctgataaatt aaatgatggt gatatatccg cattagctgg ccttctggca
     8161 aaactgtcag gaagtgttgc gctgtggtta attgatgaga gagccgaacg ctatgaaaaa
     8221 taataaactg gcgaccagtg aattaagaag ccttgccaac agcgcaatat ctttaaacaa
     8281 cgcagcaaac gaagtaatga atatgtggct tcagtcgctg gtgaataatg aacgcgatga
     8341 atcaactgca agattaatat tagccgtttc tgaattactg gatagttcaa ccagcaaaat
     8401 agagagcata tcagagaaga ttaacggctt atcaggtcac gctatcgggg aataaaaata
     8461 atgaaactta aatatcctgg cttaactgac agcggcaaaa ctcgcactaa attcatgcgc
     8521 ggggatattt accgcgacca gtacggcggc acggtaatga ttaagggcgt ggaggaacgg
     8581 cgcgtaacct accatcgtga aggctacgaa tatgattgcg tgatgcctgt ttatcagttc
     8641 cggcgtgatt tttctctggt acaggcagcg ccccgcagta aaccaaccag cagggagaaa
     8701 gcacgcgcca atattcagga aataaaaaag atgcttaacg tattcagggg taaaaaatga
     8761 aactggcacc gaacgtaaaa aaacagccac gcggaataaa acacaaagac acagaggtga
     8821 ttattttcgc gggtagtgat gcgtgggcac atgcaaaaca gtggcaggag caggacggcc
     8881 ccgcatccgg cgataatgtg cctcctgtgt ggcttgggga gcagcagtta tcagaactgg
     8941 ataagctgca aattgtgccg gaaggcagaa aatccgtgcg catattcagg gccggacatc
     9001 ttgcaccagt aatgatcaag gcgattggtc aaaagctggc ggcggctggt gtacaggatg
     9061 caaattttta tcctgagggt atgcacggtc agaaggtgga gaactggcgc gaatatctgg
     9121 cccgtgagcg gcagaatctt tctgatggtc tggttattga gtttccggta aagaaaaaag
     9181 ataccggaag ccatagcgat gacgaattaa agccacgcgt tgaaagtcgc gccgatggtg
     9241 ttttctgggt aacgcccaaa gtggataagc agtcaggcga aattatccgg cctgagacgt
     9301 ggttatgttc tccgcttgaa ctactgggaa cggggacgat cggtaaagag cattaccgcg
     9361 tgatgcgctg gaaaaaaacc gcaaaccatg aagtcatcac aatggcgatc ccgtgtggtg
     9421 gtatcggcga ccgtgacggc tggcggttac ttaaagatca cgggctgaac gtgacaacca
     9481 acggcaaata cagggcaatc ctggcggact ggatgcagtt aagcggaagc catgaggaat
     9541 ggcagttaag cacaacaacg ggctggcatt ttggcgcgta catcatgccg gacggttcga
     9601 tcattggtga atctgaaaaa ccgatcctgt ttaccggaaa aagcgccgcg attaacggtt
     9661 attccgtggc gggcacggcg gatggctggc gcgacagcgt ggcgcggctg gctggcggta
     9721 atgcctcaat gatactgggt gtggcaacgt cactggcagc gcctttaatt ggccttgtgg
     9781 gtgctgacgg cttcggggtg catcttttcg aacagtcatc ggcaggtaag accaccacgc
     9841 agaacatcgc atcaagttta tggggagagc cggacgcgca gcggctgacc tggtacggca
     9901 cggcgttagg tattgccaat gaggccgagg cccataatga cgggctttta ccactggatg
     9961 agataggcca ggccggaaat gcgcgggaag tgtcaacgtc tgcttatacg ctgtttaacg
    10021 gttcagggaa attgcagggg gcgaaggatg gcggcaacag ggagatcaaa cactggcgaa
    10081 cggtggcaat cagcaccgga gaaatggacg ttgagacatt cctcaaaagt gaggggataa
    10141 aagttaaggc ggggcagctt gtccgactgc ttaacgttcc gatggaaaaa gccacgaaat
    10201 ttcacgaata cagcaacggt aaggaacatg ctgacgcgtt aaaggatgcc tggacagcaa
    10261 atcacggggc ggcgggtcgt gagtgggtta aatggctggc aggccaccag caggaggcaa
    10321 aggacacggt aagggaatgc cgcgaacggt ggcgcaacct gataccggag agttacggcg
    10381 agcaggtcca ccgcgtgggt gaacgtttcg ccatactgga ggccgcgctt gtgctttccg
    10441 gtcatgtaac tggctgggtc gtgcaggaat gccgcgacgc gatacagcat aactttaatg
    10501 cctgggtgaa ggagttcggc acgggtaaca gggaatttaa acagatggtt gaacaggctg
    10561 aggcgttttt gtcgtcgttt gggtttagcc gctaccttcc gcaccccaat acagatgagc
    10621 gtgatttacc gattaaagag cttgccggat acagaaaagg gagcattcgc aacgaagatg
    10681 acgagatgcg ctattacacg ttcccgcacg tgtttgagag tgagatagcg aagggattta
    10741 atccggcgca ttttgcgcgt gccctggatg ctgccggaat gctggagaag ggaagcgatc
    10801 gccgatacaa aaaaaaggcg ctgggcaaga ttgggggtaa gcagcatgtt ttttacgtgc
    10861 tgatgttcca gcctgatgat gaagattaat cccctgtgtg aggaaaaaaa ttgcgggtta
    10921 tgcgggttat cctgtgtata agtgtattaa gtgtatgaat ataaaggaaa tcaataaccc
    10981 gcacgtaacc cgcaaaaggg catttataac ccgcaaaagc acgattataa cccgcaggcg
    11041 aataactgaa gtaacaccgg acagtcatgc gtaacccgca gaaaaaaccc cgtttgcggg
    11101 ttattttgag cgttttgcgg gttacgtgtt gagcaaataa taaacagggt gttttgcaac
    11161 atgctgatat aaaagggtaa aaaatggtta gcacgctaag ataacccaat aacccgcata
    11221 acccgcacat gtttctatat atataaattg aggtggcaaa tgaccagaac acgcagagac
    11281 agaacagagc cgaaatataa agcgttagat ataacagagc tggccttaaa ggtggcaatc
    11341 agaacgatag accgacacgt aggggaagga tacgcgaagg aacatcccga cctgataagc
    11401 gcattcatga ccacagcagc ggcaaacttt gccacgttga cagaacggga gattgccgaa
    11461 gcggaacagg taacaaccat caacgttaaa accggagagg tggaatcatg acagcacaga
    11521 tagcggctta cgggcggctg gtggacgacc cgcaggtaaa acagaccagt aagggcacac
    11581 cgatgacgct ggcgcgtatg gcggtatcgt tgccatgtag tcaggcgcag gatgggcagg
    11641 cgacgttatg gctatcggtc atcgcatttg gtaagcaggc cgacttcctg gctaaacatc
    11701 aaaaaggcga cgttgccagc gtatccggca cgatgcaggt cagccagtgg accggacaga
    11761 acggggaaac gcggcagggt tatcaggtta ttgcagacag cgtaatcagt gcccgtgcgg
    11821 cacgtcctgg cgggaacaga cgcaaaacca caggcacaca gggtaatcag ccaccagcgg
    11881 gaggcgatga cccttacggt gacggtattc cgttctgagg gggtggcgat ggtacatgac
    11941 cgcatagcgg aagaactcga ggcgaaaggc ttttaccgga gggcggcggc gcgatggggt
    12001 gaagtcatgc tgctggtgga gacagacaag gaacggcatc aggttacgat gcgacggctg
    12061 gaatgttcca ggaaggcaca gaagccaccg gagccgccgg ataacttcgg agacctgaga
    12121 aaggcagtcg atcgcactta tgctgaaatg ggtatagatg gtgtaagcga tgaaatatgg
    12181 cgtaattacc cagacagcta atcaacagcc ggagaaatcc ggcttttttt gcaccagttg
    12241 aaacggtatg gcgcattacc gggttttcgt cacggtcagg catagttact atctgaaaca
    12301 aacagacaca acagaggaaa aaacaatgcc gatgaaattt gatgagatat taaaacagcg
    12361 tgataaatac catgctgaca acatggagac gatgagcatc aatgattacc gcgcattcct
    12421 ggagacgggc gcactgattg aaaaggatca gcatggtttt gtgagatgtg ctctgtccgg
    12481 tgaaatgctg gcggtaaatc ctgaacagat agatgcattg atagaatttc tgaaagggat
    12541 cagggactga gcacgcatac agccggagca atccggcttt ttgtcatttt ttgtaaatta
    12601 tttgttcgtg gttgttccac gttgttcact gacaggatcg gcatatttta cccgaactga
    12661 atcatgagta ttctcgcccg tggtgccagg acgctggggc cactttcccg cctgttaatg
    12721 tgctcgccaa tattcattac caggcgggaa aacgatcggt gcgattgctg atttccttat
    12781 gaaaaacggt tgagtttttg ccgcgtcctg gagttcctta cttaacccca ggactttttt
    12841 ttatgccgag aataatcgaa ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg
    12901 acatgctgga gaacgcggaa aaagaaaacc gcagccttaa cgatgcagag ggcgcaaaat
    12961 ttgacgaatt acgcgctaaa gctgaatccc tcgataaaga catttcccgc cttgaagcca
    13021 ttgcagacga agagcgcagc aagccaggta aaagcagcca gaccactgac ccggcagaac
    13081 tccgcaacta catcctgaca ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg
    13141 gtggttatac cgttatcccc gaactgaaca ccgaaatcat gcgaatgctg acggatgaat
    13201 ccaccatgcg ccgcatctgt accgtgaaga aaatcagcag caacgagttt aagcagcttg
    13261 tttccgctgg cggtgcgacc gttaaccacg gtgaagaggg taagacacgc gaacagacca
    13321 gcaccccgca gattaacgag gtgagcatta agctgtatcc ggtctatgcg tacccgcgca
    13381 ccacacagga aatcgtggat ttttccgatg tggacatcct ttcatggctg acgggtgaga
    13441 ttggcgacac cttcacggaa accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa
    13501 aagcaaaagg ttttttatcc gtaccccgtg cagagaagaa cgacaaagag cgtgattttg
    13561 gtacgttgca ggtaattaaa ccttccgaat ctctggcgtg gacatctgcg gacccgctga
    13621 tcgacctgaa atttgcatta cgtaaaaaat accgcaaaaa cgcggtctgg gtggttaact
    13681 ccacgacggc ggcaaaactt cagaaggtga agaacgcgaa cggtgattac atctggcgcg
    13741 accgtttaca ggcgggtgat cctgatacgt tgctgggcct tccggtcgaa tatctggagt
    13801 ttatgcctga taacgttatt gccctgggtg acttcaaacg cggttactac attgttgatc
    13861 acgaaacagg tgttcgcacc agaccggaca acctcacaga gccgggtttc atcaaaattt
    13921 tcacgcagaa atatttaggc ggtggcgtgg tggattcgaa cgcgatcaag attctggaac
    13981 tgccacagga cgacgattaa cagcatacag aaggggctta aaagcccctt tagtgtttta
    14041 tgggtgaaaa aattatgaag agtatggaaa tccggtcatc ggaaatcacc accagcgcca
    14101 gcaacacgct tacaggctac gttgttcgct gggataacct ttcagagctg ctatgggggg
    14161 agttttacga aaaattccag cggggagcgt ttactgaatg gcttgcggcg ggtaatgacg
    14221 ttcgcggcct gtatgagcat gaccacagca tgttactggg gcgcacccgt tccggcacgc
    14281 tgaaactgga agaggacgaa acagggttac gctttgaact gaccccaccg gataccagta
    14341 cagggcgcga cgttatcgaa ctggttaagc gtggggacat ctcggggatg agctttggct
    14401 ttcgctcccg taaggatgta tgggatacca caacagatcc atgcgtgcgc accgtgctgg
    14461 tggcggaact gtacgaaatt accgttacat cggtaccggc ttaccccgat tccggcgtgg
    14521 agctggcccg ccgttccctg tatgagcagc accccgaaaa aatgccgcgt gcggataatc
    14581 gccgctggtg ggcggattta gcgggggtgt gatatgtggc ctttcagaag aaaaaaagag
    14641 cagcgcagca tgacgcttga tgagtttatg gcgctggctg gcacatcgaa cacgggggcg
    14701 ggtgagtacg tatcatcggg gacagcggaa tcactgcccg ccgtcatgaa cgccgtgacg
    14761 gtcatctctg aggcggtggc taccatgccg tgttacctgt acctggtacg caatgagaag
    14821 gggaaggagg cccgcgagtg gcttgattct catccggtcg atcatatcct caacgagcgc
    14881 ccgaacgcgt ggcagacgcc ctatcagttt aagcgaatga tggtccgcca ctgcctgtta
    14941 aacggtaatg cttatgcggt gattcagtgg gggcgtgatg gttttccggt ggctttgcac
    15001 ccttacccgc cgcagtcggt gaacgtggag cagacaggcg aacacaactg gcgctattgc
    15061 atcactgacg cctacaccgg aaacacccac aactatttac cgtgggaagt acttcacctt
    15121 cgttactcca cggatgacgg ctttatgggg cgctcacctg taaccatctg ccgcgaatcg
    15181 ctggggcttg ggctggccca acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg
    15241 gcggcagggg ttatcacgtc aggcgaatgg ctggacggcg tgaagggcaa acaggcatta
    15301 gccgcactgg aacgctacaa aggggccaga aacgccggaa aaacgcccat ccttgaaggg
    15361 ggtatgagct atcagcagct gggcatgagt aatcaggatg ctgaatggct ggcctcccgt
    15421 cgctttacca ttgaagacat cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa
    15481 tacagcaaca gcacctacag caatttcagc gaggcaagcc gcgcatttct caccatgacg
    15541 atgcgcccgt ggctggcgaa ctttgagcag cagataaaaa acgccctgct ggtggcctcg
    15601 cctgtacctg gtatccggta tcaggtggag tttgacagcg cggacctgtt acgggccaca
    15661 cctggcgaac gctttgccac ctatgagcgc ggcatcaaat ccggcgttat gtgcccgaac
    15721 gaagcccgcg aacgtgaagg actgtcaccg cgtgatggtg gtgatgagtt cagccaggca
    15781 tggaaacagg aagtaaaaat cagcgaggga gaaaaaccgg aatgaacata gggcgactgc
    15841 gtgacaggat aacgattcag accctgaaac agaccagagc catgacgggc gaaatactcg
    15901 aaacgtggga ggacggtcac acactctggg caagcgtgaa catgatcagc agcaaggagg
    15961 ccatttcatc gggcgcagag ctggcgattg gtaccgtaag gatctggata cggtaccgga
    16021 aggacatcaa cgccaccagc cggataaagg tcagtacggg gccgctggcg gggcgtgtac
    16081 tgaatatcat cgggcagccg ttgccggatg tcgccaggac acgccttgaa attctttgtc
    16141 gtgagggcgc ggaaaaatga cagaagaact tatcaccctg gaagaagtga aactccattg
    16201 ccgcatcgat ggcgacgagg aagaccagtt aatcagcgga tacattgccg catcgcttga
    16261 ggcgtgccag atacacatag gcaggcgctt tgatgacggg ctggagttca cgccagccat
    16321 aaagattggc tgcatgatgt ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga
    16381 taacgcaaaa acacgcgtac ccatgacgat tggtgcactc tggacggctt accgtgatgt
    16441 gggggtgtac tgatgccgtg gcaaccatta agacgatgca cagaaccagg ctgtaacagg
    16501 cgcgtgaagt ccggcaagtg tgaggagcac aggcgggcgg catggcgtgc agaggatgcc
    16561 agacgaggac accgccgcgc gcgtgggtac tccagacagt gggacaaata ccgcgccatg
    16621 tatctgagta aaaacccgtt atgcgtgcga tgccttgaga aggggatata tacgcccgcc
    16681 gtggtggtgg atcacattat ccccatcgat ggcggtgatg atgttctctt ctggcccgaa
    16741 tggaaccatc aaccattgtg tcaggcgtgc cataaccaga aaacgaaatg gcttgatccg
    16801 gcaacaaaaa gcaagcgtgc cgcaggtgga tttcgtgaag aggaagaacg ggccgctaac
    16861 cgcaataact ggatgtatga cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg
    16921 acttataagg cagcgtgacg catggaagac acaggaaaca gggcataaag ataaagcgtc
    16981 aggacgcgca gaacgcatca cagcgaagcg attaaccgac cgtgaccgcg aggttatgga
    17041 atgtttccgc aatcgctgat gaggctgtat gacggggtgg ggggcgtttt caggacaaac
    17101 ccgaccccgc cgggcaccga acgcctcctc aaatttttat gcacgggaat tttttgaaaa
    17161 ataatctggc gaaaaaataa gcatggcaag accaccgaaa gcccccgcct acctggatga
    17221 tatcgccgtg aagcagtggc gggaaaaatc gcggcagctt gcggaacggg gtgacctgac
    17281 tcccgccgac tggagtaatc tggaactgta ttgcgtcaat tactcgatat accggaaagc
    17341 cgtcgcagac cttgcggcgc gcgggttcag cattgttaac agtcagggcg gagagagcag
    17401 aaaccccgca ttaagcgcga aatccgacgc ggaaagagtg atgataaaaa tggcctcctt
    17461 gctcggtttt gacccgataa gccgccgtaa aaatccaccg gaaacagaag aagaggacga
    17521 gcttgaccgc ctggaataag tacgcagaag acgtaaaaac gggcaaaatt ccggcctgta
    17581 aacggttaaa acaggctgtt aaacggtact tttcggacct taaaagcccc ctttacacgt
    17641 tcgatcgtga ggttgtggag cggtttattg ccttttccag ggtgtgcccg cacgtaaaag
    17701 gaccgatgcg tggcagaccc attgagctgg agccgtggca gcagttcgcc tttgcgtgca
    17761 tcctcggctt taaggttaag gccaccggac ggcgcaaata cacgagcgcc tttatcgaag
    17821 tgccgcgcaa aaatgcgaaa tccacgaccg ccgcgattct ggctaactgg tttttgatta
    17881 tggagaacgg tcagcaggat atctacaccg cagcggtgag ccgtgaccag gcgcggatcg
    17941 tctttgatga tgcgcgtcag atgtgccttt tatcccgacc gttacgcagg cgggtgaata
    18001 ttcaggcgca taaggtgata cacccgaaaa gcaacagcct gttaaagccg ctggcagcaa
    18061 aagcggcaac cattgaaggt acaaacccga gtcttgccat tgtggatgaa tatcacctgc
    18121 accctgacaa cggggtttat tccgcgcttg aactgggaat gggggcgcgt ccggaggggc
    18181 tgttatttgc catcaccaca tcggggagca acgttgtttc agcctgtaaa caacactacg
    18241 actattgctg ccagatactg gatggtgaag aggtgaacga atccatgttc gtgctgattt
    18301 acgagctgga tgatgaaagc gaggttgacg atccggcgat gtggataaag gcgaatccca
    18361 atatcgatgt ttccgtcgat cgtgaaaaac tggcctcaac catccagaaa gcgcggggaa
    18421 taccgtcgca gtgggtggag atgatgacaa agcgatttaa tatctggtgc cagggggcta
    18481 ccccgtggat gggtaacggc gcatgggctg agtgtgccgg aacgttcacg gaggaagatt
    18541 tacacggcca ggagtgttac gcggggctgg atttatcatc aaccagcgat atttccagcg
    18601 tatgctatgc ctttccggtc ggtaaaacca ttatgctgat ttcccgtcat tatctgccgg
    18661 agttccagct acagaacccc gccaataaaa accgcgctgt ctatcgtcag tgggctaaag
    18721 cgggctggat acgcacaacg cctggcgact gcattgatta cgaccggatc agagatgaca
    18781 tcatgcagga cgcggagaaa tttcatatca ggctggtggg ctttgatacg tggaacgcca
    18841 cgcatctcag aacgcaatta cagggggcag gttttgaggt ggagccgttc ccgcaaacct
    18901 acctcagatt cagtccggcg gcgaaatcgt tcgaagtttt tgttaaccgc agggtgattg
    18961 tgcatcgtgg cgatccggtg ttgtcctggt cgatgagtaa cgtcgtgatg cagagtgacg
    19021 cgaacgccaa tatcaagccg aacaagaaaa aatcaccgaa caagatagac ccgagcgtgg
    19081 cggcactgat ggcgtttggt acattccagg cagagcatga ggattttgct tttgatataa
    19141 gcgacagcca ccgccagaaa ctggaagaat tcagcggggt gtaatgaggt cagcagcatg
    19201 acagaagccg aactactggg attaatccgc cgcgtatctg gaatcagcca gcaggctgac
    19261 gaacagacca cgcagccgga cagcgtgaca gccgaaaatt atgcgcgtgt tgttgctgag
    19321 gtgatgtgcc gtgatggtat ccagcttaat gatgttgata tgcgcaacat acggatccgc
    19381 gttcttgaaa tgctggccta caatcgccgc gttgaactgc atcgggagaa agaaaaaata
    19441 acgtaccact ggaagaagcc ggagcggttg cggcggtaac tggttgatat tttcgaaatt
    19501 cgccagcggc gaacatcggg gaaaccagcc taacccgttg attctttcgg aaacagccat
    19561 tggcggaatc cggcgcggta acttgctgat attcccgata acgcaaaatt gcgtggctga
    19621 atgaacatta tgattactta tgattacgca gatgattaag gcctaaaccg ttgattcttc
    19681 ccaatttcca cagactgtgg aagttggcgc ggtaacttac tgagattaaa gcaaagcgca
    19741 aaattgcgcc gactacatcc ctcacatgcg aaattttcgc agtttaatta ctcgccagtt
    19801 tgatcgttaa cccgctgata ttttcgggaa cctcaatttg aggaagtcgg cgcggtaact
    19861 ggttgatact ttcgacctcc tcaatttgag gaggtcgggg cacagaaaaa caacgaatat
    19921 gttgtttttg ggaatggtca taatgaccac gcagaccatc aaggcaacca caacgaacat
    19981 cgttacggtt ggagctttta ccccactggg ggaaaggtat tacgataatc ataacaccta
    20041 ccgaacaggt aaaacccacc agcctgatta acagttaacc ggaaaaaaaa tccggtagcc
    20101 ccgttatggg gatccccata tcgacattaa cgcccctcat gaattggtgc gcttccccct
    20161 ggaaagatta cccgcctgta tatttcttgt gtctatttgt tccacgttgt ttcatacagt
    20221 gcaccgaacg gtgtagttaa tggtgtagtc aatttgctgt ttttagcact ttttgcagtg
    20281 tgggttgttt aattaaatca atgggttgcc ttgctgtggt gataaaagga catatttatt
    20341 catctttcgg cgtagaatgc tggcgaccaa aaatcacctc catccgcgca ccgccaagca
    20401 tgctctctcc ggcgacgatt ttaccctcat attgcttggt gatttcgcgg gctaccgcca
    20461 gccccacacc ttgcccaggg cgtaaagtat caacccgttg accacggtcg aaaatgacct
    20521 ctcgcttgct taatggaata ccggggccat catcctcgac cacaatatag agatgctcgt
    20581 cggtttgcct tgcagaaatt tcgacaaact cgaggcaata tttacaggca ttatccagta
    20641 cattgcccat cacctcgaca aaatcgttct gctcaccgac aaagctgatc tctggcgaaa
    20701 tatcgagaga gatattgacc cctttgcgtt gatacacttt gttcagcgcc gaggtgagat
    20761 tgtccagcag tggggcgacc ggatgcagct cgcggctgag caatgtcccg ccgtgcatac
    20821 tggcacgatg caggtagtag ccaatttgct gtgaaatgcg gctgatttgc tccagcatta
    20881 ccggttcagc atcactgacg ctcatctttt cactacgtag agaacgcagc gtactttgca
    20941 gcaccgccag tggcgttttc agactatggg tcaggtcggt gagcgttgta cgatatttgt
    21001 cgtaacgttc gcgttcactt tttaacaatc ggttcaggtt tcgtaccaga ctggtcagtt
    21061 ctcgcgttgt ggctggattg agcaattcgc ggttatgttc ttccagttcg cggacttctt
    21121 ttgccagggc ttcgatgggg cgtaaactcc accaggcggc gacccacagc agcgggatca
    21181 ctaacagcag attggctgag agcacataga taaaccagct ccagaccata taggaacttt
    21241 ttagctccac cggaatggta tccaccacca caatggttaa ctttggcatc cgcgatgttg
    21301 ccgggtagac gtttaccgcc accgagtggg tcatctccgc gtcgtcatca tcttcccgca
    21361 cttcctgcaa ctgttgctgt atcgaatgat ctccactcag caagaggctg gtatcgttaa
    21421 catccgcttc aatttcatga aaaccattcg atttcagcca gtcaggctgg atcatcttca
    21481 tcagccaggg cacgtcacgt tgcgcccata aaagctgccc gttctcatca taaattagcg
    21541 tcatggtggg gctttgcttg tcgatatttt cgggtaactc gacatgcaac ttattgtttt
    21601 cccacttcgc aagggtatag aacagattgc tctcgccacg taacagccga aacgtagttt
    21661 tatcgaaact gacgctataa ccgatcagcg cgaccattcc gtaggcaagc gaaagcacca
    21721 gtactaccgc tgccgttgcc aacagaaaac gtacccgcag cgagagcggg aaaaaaagac
    21781 gcagtaattt tttcatcagc gcaattcgaa caaatagccc tggccgcgaa cggtagtaat
    21841 cacttcttgc ggatattgtg cctgaatttt tttgcgcaga cgtcccatca gtacatcaat
    21901 ggtatggctt tcccgcagct ccgcatccgg atagagttgt agcattaacg aatctttgct
    21961 gaccactttg ccattattgc gtatcaacgt ttccataatg gtgtattcga acgcggtcag
    22021 tttgatcact tcgtcattaa tagataattc acggcgagag agatcaacct gaaacggggg
    22081 gagcgaaatg acctgtgaag ccaggccgct attacgccgc attaatgcct gcattcgcgc
    22141 catcacctct tcaatatgaa acggtttagt cacgtaatca tcagcaccgg cacttaatac
    22201 ttcgactttg tcctgccagc tttcacgggc ggttaatacc agaatcggca gtgaaacatc
    22261 attgctacgc cagcggcaaa tcagtgacag accgtcctcg tctggcaatc cgagatcgac
    22321 aatcgcaata tccggtaaat gttcattgag ataataatcg gcttctttgg catcttctgc
    22381 atcatcgacc tgatgaccag catcctgaat ctgaacttta aggtggtgac gtaacaacgc
    22441 attgtcttca acaaccagta cgcgcatttt tatttctccc tgtcttaatt attaaaatag
    22501 tgtaacgcga ttatgtggtt atgggggtaa acattaaata aaccagcggg ggaggggagg
    22561 taaagtgaaa aaataaaaag cggataatct taataagcag gccggacagc atcgccatcc
    22621 ggcactggta cgagtttatt tcagctcatc aaccatggtg atggcgcgac caatgtagtt
    22681 agccggcgtc atcgctttca ggcgggcttt ctcttcttct ggcaacgcca gaccgtcgat
    22741 aaactgcttc atgccttcgg cgtcaacgcg cttaccgcga gtcagctctt tcagcttctc
    22801 gtacggtttt tcgatgccat agcgacgcat aactgtctgg attggttctg ccagcacttc
    22861 ccagttgtga tccagctcat ccagcagatg gtcacggttc acttccagtt tgctcacgcc
    22921 tttcagggtg gattgatacg caatcagcgc ataaccgata cccacgccga ggttacgcag
    22981 cacggtggag tcggtcaggt cacgctgcca gcgggaaacc ggcagtttgc ttgccagatg
    23041 ctgcaatacc gcgttggaaa ggcccagatt cccttcggag ttttcgaagt cgatcgggtt
    23101 aactttatgc ggcatggtgg aagaaccaat ctcaccagca atggttttct gtttgaagtg
    23161 gttaagggcg atataacccc agacgtcacg gtcaaagtcg atcagaatgg tgttgaagcg
    23221 cgcaacgcaa tcaaacagtt cggcaatgta gtcgtgcggt tcaatctggg tagtgtacgg
    23281 gttccattga atacccagcg aggtgacgaa ctcttcgctg aactgatgcc agtcaacttc
    23341 cgggtaagcg gcgatgtggg cgttatagtt accgaccgcg ccgttgattt tgccgaggat
    23401 ctccacctgg ttaagctggc ggtactggcg ctccatacgg taggcgacgt ttgccatctc
    23461 tttaccgatg gttgacggcg tggctggctg accgtgggta cgggacagca gcgggatatc
    23521 gcgatactga acggcgagat ctttaatgcc atcaattagt tgacgccagt acggcaggat
    23581 cacttcatca cgcgcggttt tcagcattaa tgcgtgggag aggttattga tatcttccga
    23641 agtacaggca aagtggatga attcagaaac cgcgtgcagt tccgggatct ccgccacttt
    23701 ttctttcagg aaatactcaa ccgctttaac gtcgtggtta gtagtacgct cgatggtttt
    23761 gatgcgtgcg gcatcttctt cgctgaaatt ggcgacaatt gcatcaaggt aaccgattgc
    23821 gtcggcagca aaagcaggaa cttccttgat cgctgcgtgc gcggccagtt tttgcagcca
    23881 acgtacttca acttgtacac ggaatttcag caaaccatat tcgctgaaaa tcccgcgcag
    23941 cgcgctgact ttatcgccgt agcgtccatc gacaggggaa acggcggtca gtgaggataa
    24001 ttccatagat cacaactccg gggttaaatg agcaagaatt tgttttgcct gagtggtcag
    24061 gcgattacga gaaaacatca gttgcagacg tccgccgccg acctggtgcc agagcacggc
    24121 ggcgcgaatg cctgccagca gggttgcgcg aactttcgcc tgcacttgtg ggctttgcag
    24181 tacagcaggg gaaccggtga cctgaatgcg cgggccaagc gggctgatca catcaacata
    24241 gatagcagcc atcgcgctca tcagcgtttc ggactgtaaa tcgaagtgtt cgagctggcg
    24301 ttgcaggccg ttgatccggt tgcccagagt gtcgagcgca cctttcgctg aggagagttt
    24361 gcgcttaagc accatcaggc tgagtgtgta gcgggttaat tcggcgttta agccctggcg
    24421 actgctggca ttgagcacgc cgagcagggt ttccagcccg acgcgcaggt tggcttcgct
    24481 accgccaaaa accgccagcg tcgagctggg gttcatatca ataatactgt tgagtgagac
    24541 gtgtagcgca tcggcatcac aatgcccctg gtgagcgagt tgttgcacca ggcgtgccga
    24601 ctgacaaata ccggccaggg cgagggtgat gtcatagtaa ttctttgcca cgttcactgc
    24661 ttccttgttt taagtaaaga taataatcag atcggcagcg gcagacgctg ctcaataata
    24721 ccgccaccga ggcacacttc accgttatag aagacggcag actggcccgg cgtcacggcg
    24781 gcaacgggct catcgaaaat cacttcaatg cgatcatcgt ccagcgcctt gacggtgcag
    24841 gggatatcgg tctggcgata gcgggttttt accgtgcaac gcatagtacc ggtgaatggt
    24901 tcacgatcga cccagtgcag ctgctgggca atcagaccga cagacatcag acgcgggtgt
    24961 tcatggccct gcgcaacaat cagaatgttg ttttcgacgt ctttatcgac cacataccac
    25021 ggttcttcgg tgccttcttt ggtgccaccg atacccagac ctttgcgctg accgagggtg
    25081 tgatacatca gcccctgatg ctcaccaatt tcatcgccat cgactgtaat gattttgccc
    25141 ggttgcgccg ggagataacg gccaaggaac tcgcggaatt tacgttcgcc aatgaagcaa
    25201 atgccggtag agtctttttt cttcgcggtg accagaccaa gatcttcagc aatcttacgc
    25261 acctgcggtt tttccagttc gccgaccggg aacaggcttt gcgcaatctg ctcatggctg
    25321 agcgtataaa ggaagtagct ctggtcttta ttgctgtcca gaccacgcag caggcggctc
    25381 ttgccatcga catctgcccg acgaacgtaa tgaccggtag cgatataatc ggcacctaaa
    25441 tcttcggcgg caaattcgag gaaggcttta aatttgatct ctttgttgca cagaatatcc
    25501 ggattcggcg tgcgaccggc tttatattcg gcaaggaaca gttcgaagac gttgtcccag
    25561 tactcggcag caaagttaac ggtgtgcagt tcaatgccga gcttgtcgca gacagcctgg
    25621 gcatcagcca gatccgctgc cgctgtgcaa tattcctcac cgtcgtcttc ttcccagttc
    25681 ttcataaaca ggccttcgac ctgatatccc tgttgttgca acagccaggc agaaacggag
    25741 gaatcgacac cgccggacat gccgacgatt actttttttg cggtttcaga cattggatca
    25801 ctcacgacat tgaacttcaa ggcggcgtat tctatcacgc agccttttag atgacaccct
    25861 ttgtaaaagg ccagttaaaa tcgccaatca tctccagcgg atagcgttgc ccgctttgat
    25921 aacaacggat actttccgcc accagcggcg aacgaagatt tgacgcctgt aaaatttctt
    25981 cggcgctgac ccaacgacag cagtcgatat cgctgtcatg aggctgggtc gggcatattt
    26041 gctcaagctc aatggcaaag aggaaacgca aaaacggcgt tttatctggc gcaatccact
    26101 gatgcatacg aataaagtgt tgcggctgcg cgctgatgcc ggtttcttcc cacagctcac
    26161 gggcggctgc ttccactaag gtttcatcgg cttccagatg cccggcaggt tggttccata
    26221 aggctttacc attaatcgtc tcttcaacga ctaaaaattt cccctctgcg tgcactacgc
    26281 aagcaacggt aacgtgcggt ttaaacattg tgcatcctta ttctgttgct tctcgccatt
    26341 cgccattggc aaggttatcc aaagagtaat cacccatcgc atagcgaatc agtcgcagcg
    26401 tggggaaacc aacatgggcg gtcatgcggc gcacctggcg attacgtcct tcatataagg
    26461 tgatcttcag ccagctggtg ggaatacttt tgcgttcacg aattggtgga ttccgcggcc
    26521 ataaccacgc gggttcgtca accagctccg cgcctgcggg cagggtaggg ccatcattta
    26581 aggttacgcc attgcgcaag gcttcaagtg cgtcttgtgt gggaatacct tccacctgca
    26641 cataatagat ttttccggtg cgtttgcccg gctgggttaa acgcgcctgc aacgcgccgt
    26701 tattggtcag caccagtaac ccttcgctat cgcggtcaag acgacctgct gcataaacgc
    26761 cctgaaccgg gatgaattct tttaatgttt tgcgtccggc ttcatcggtg aactgcggaa
    26821 gaacatcgta gggtttattg aacaggatca cacgcgtggg ctggttttca ggtttacgcc
    26881 tggtagaacg ttgtgagctg aatcgcttaa cctggtgatt tctaaaagaa gttttttgca
    26941 tggtattttc agagattatg aattgccgca ttatagccta ataacgcgca tctttcatga
    27001 cggcaaacaa tagggtagta ttgacaagcc aattacaaat cattaacaaa aaattgctct
    27061 aaagcatccg tatcgcagga cgcaaacgca tatgcaacgt ggtggcagac gagcaaacca
    27121 gtagcgctcg aaggagaggt gaatggaaag taaagtagtt gttccggcac aaggcaagaa
    27181 gatcaccctg caaaacggca aactcaacgt tcctgaaaat ccgattatcc cttacattga
    27241 aggtgatgga atcggtgtag atgtaacccc agccatgctg aaagtggtcg acgctgcagt
    27301 cgagaaagcc tataaaggcg agcgtaaaat ctcctggatg gaaatttaca ccggtgaaaa
    27361 atccacacag gtttatggtc aggacgtctg gctgcctgct gaaacccttg atctgattcg
    27421 tgaatatcgc gttgccatta aaggcccgct gaccactccg gttggtggcg gtattcgctc
    27481 tctgaacgtt gccctgcgcc aggaactgga tctctacatc tgcctgcgtc cggtacgtta
    27541 ctatcagggc actccaagcc cggttaaaca ccctgaactg accgatatgg ttatcttccg
    27601 tgaaaactcg gaagacattt atgcgggtat cgaatggaaa gctgactctg ccgacgccga
    27661 gaaagtgatt aaattcctgc gtgaagagat gggcgtgaag aaaattcgct tcccggaaca
    27721 ttgcggtatc ggtattaagc cgtgttctga agaaggcacc aaacgtctgg ttcgtgcagc
    27781 gatcgaatac gcaattgcta acgatcgtga ctctgtgact ctggtgcaca aaggcaacat
    27841 catgaagttc accgaaggcg cgtttaaaga ctggggctac gagctggcgc gtgaagagtt
    27901 tggcggtgaa ctgatcgacg gcggcccgtg gctgaaagtt aaaaacccga acaccggcaa
    27961 agagatcgtc attaaagacg tgattgctga tgcattcctg caacagatcc tgctgcgtcc
    28021 ggctgaatat gatgttatcg cctgtatgaa cctgaacggt gactacattt ctgacgccct
    28081 ggcagcgcag gttggcggta tcggtatcgc ccctggcgca aacatcggtg acgaatgcgc
    28141 cctgtttgaa gccacccacg gtactgcgcc gaaatatgcc ggtcaggaca aagttaaccc
    28201 aggttctatt attctctctg ctgagatgat gttacgccat atgggttgga ctgaagcggc
    28261 tgacctgatt gttaaaggta tggaaggcgc aatcaatgcc aagaccgtaa cctatgactt
    28321 cgaacgtctg atggaaggcg ctaaactgct gaaatgttca gagtttggtg acgcgatcat
    28381 caagaatatg taatcactac atgtgttaaa tattgaaacg ggcgtataac acgcccgttg
    28441 ttttatgatg atttaaaatc ttccccaaaa ctttccccaa aacccttccc caaaactggc
    28501 tattttctat gctgttttga tatctacgat aatccagtct ttaccacgat catcattgta
    28561 tcggtcggtc atttttctgg atttatgacc taacaacttt tgcgtatcca gaccctgttc
    28621 ccgatataac cgttctgaca gagatcgctg ctcatgaaat gtgggcgcag ttccttgctc
    28681 ccattttatg ccacattttt ccctggcctt tttaaaagcc gttgtaagag tatttgcaga
    28741 cacctggtct cctctgtttg cttgagaggt agtgtgacgg taatggacca gatatttact
    28801 aacaacagca tccctgcact gagatataac ttcacgaagg gtaatattca gagcatcgca
    28861 tttcaggtta agcggaatag caagttttga accggttttt tcctgagtaa tgtgcaacat
    28921 gtcgtcccag atatcagaga atttcaaatt gcagatatcg cttaaacgtt gtccagtgac
    28981 aagagcaagt agcatcccgc attttaaata gggctgccgt ctgcttacgc tgtcaaatat
    29041 tgcctgccat tcgggcagtg ataatctttg acggtttact cgatttcgcg gttgttttgt
    29101 tgcctgcgct gggttaaatc ctggcggaac atgtcctgcg tgttgtgctt ctttgaagac
    29161 gtcgatcaac accattctca cgacttgcgc catcctgtta tgaccttcag cctttacagc
    29221 atcaattatt tcggcaatat caagtgcggt aatatccttg aggtgttgca ttccacaatg
    29281 ctcacggaaa agacggatgg gtttgccttt ttgccgatag gagttgggtc ttagttcatt
    29341 atgttgcagc ctgtcctcct ggatagaatt atatttatca agccattctg tcaccgtaat
    29401 gtctgagcgc ctgcctttca ttctttccag acgctcattg acgcttaata tttgtcgggt
    29461 acgttgttca gcaataatgg tatttgcttc agtagcaact tgttttgctt cattctcatc
    29521 agttcctaag ctatgaaaac gaccggatag tggatgtttg tattgccaat atacctttcc
    29581 ggttcgctta tctaatttgc aatataaatt gggtatagag attttgtgag atcgggggct
    29641 agcagccatc agcgattatc cgttggagtt ttgggtttgc gtttattggg agttgcggtt
    29701 ctgcaagcgt tcctacaaaa cgggaatttc ggtcaatcat ccagtagcga ccaactttta
    29761 tagcgggtgg ggccatcatt ttcccttgcg cgtatttttt cagaactcgc tcacttggtg
    29821 ctaagtcccc aaattcttct ttagcccagt cccgtaaagt gattagtcga gacatttgtc
    29881 ctcctcttag ctgctgaggg agtttgtgac cgatatatct gacatgatat taagctcatg
    29941 gcaggtacat ctcttgactg gtcatagaga taaatttaat gctgagaaat gcagtattga
    30001 atttatcaat ttttcaccag cttctggtct ttctggctgt catgcgcaaa acatatggct
    30061 ttaataaaaa actggattgg gttagcaacg agcaactttc cgaattaacc gggatattgc
    30121 cgcacaagtg ttctgctgca aaaagtgttc tggtaaagcg tgggattttt attcagagcg
    30181 ggcggaatac cggcattaat aatgtggtca gtgaatggtc aacattaccc gaatcaggta
    30241 agaaaaataa agtttacctg aaagaggtaa atttacctga atcaggtaaa aaaagtttac
    30301 ccaaatcagg taaaggcgtt tacccgaatc aggtaaacac aaaagacaaa ctaacaaaag
    30361 acaatataaa acctttttcg tccgagaatt ctggcgaatc ctctgaccaa ccagaaaacg
    30421 atcttcctgt ggtgaaaccg gatgctgcaa ttcagagcgg cagcaagtgg gggacagcag
    30481 aagacctgac cgccgcagag tggatgtttg acatggtgaa gaccatcgcg ccatcagcca
    30541 gaaaaccgaa ttttgcaggg tgggctaacg atatccgcct gatgcgtgaa cgtgacggac
    30601 gtaaccaccg cgacatgtgc gtgctgttcc gctgggcatg ccaggacaac ttctggtccg
    30661 gtaacgtgct aagtccggcc aaactccgcg acaagtggac ccagctcgaa atcaaccgta
    30721 acaagcaaca ggcaggcatg atagccagca aaccaaaact cgacctgaca aacactgact
    30781 ggatttacgg ggtggattta tgaaaaacat cgccgcacag atggttaact ttgaccgtga
    30841 gcagatgcgt cggatcgcca acaacatgcc ggaacagtac gacgaaaagc cgcaggtaca
    30901 gcaggtagcg cagatcatca acggtgtgtt cagccagtta ctggcaactt tcccggcgag
    30961 tctggctaac cgtgaccaga acgaactgaa cgaaatccgc cgccagtggg ttctggcttt
    31021 ccgggaaaac gggatcacca caatggaaca ggttaacgca ggaatgcgcg tagcccgtcg
    31081 gcagaatcga ccatttctgc catcacccgg gcagtttgtt gcatggtgcc gggaagaagc
    31141 atccgttatc gccggactgc caaacgtcag cgagctggtt gatatggttt acgagtattg
    31201 ccggaagcgt ggcctgtatc cggatgcaga gtcttatccg tggaaatcga acgcgcatta
    31261 ctggttggtt accaacttgt accagaacat gcgggccaat gcgctgactg acgcggaatt
    31321 acggcgcaag gctgccgatg aactgacctg tatgacagcg cgaattaacc gtggtgagac
    31381 gatacctgaa ccagtaaaac aacttcctgt tatgggcggt agacctctaa atcgtgcaca
    31441 ggctctggcg aagatcgcag aaattaaagc taagttcgga ctgaaaggag caagtgtatg
    31501 acgggcaaag aggcaattat tcattacctg gggacgcata agagcttctg tgcacaggac
    31561 gttgccgcgg taacaggtgc aaccgtaacc agcataaatc aggctgcggc taaaatggcg
    31621 cgggcaggaa tcctggtcgt tgatggtaag gtctggcgaa cggtgtatta ccggttcgct
    31681 accagagaag aacgggaagg aaaggtgagc acgaatctga tttttaagga gtgtcgccag
    31741 agtgccgcga tgaaacgggt attgagggta tataaaagaa catcaatggg tacacaatga
    31801 tgaaacaggt gagttgagtt caaactgtag tacaattctc tccagtttga acaggaaaga
    31861 atatgttatg aacccttata tttatcttgg tggtgcaata cttgcagagg tcattggtac
    31921 aaccttaatg aagttttcag aaggttttac acggttatgg ccatctgttg gtacaattat
    31981 ttgttattgt gcatcattct ggttattagc tcagacgctg gcttatattc ctacagggat
    32041 tgcttatgct atctggtcag gagtcggtat tgtcctgatt agcttactat catgggggtt
    32101 tttcggccaa cggctggacc tgccagccgt tataggcatg atgttgattt gtgccggtgt
    32161 gttggttatt aatttattgt cacgaagcac accacattaa aaataatttc ttttaaaaga
    32221 ctgcaatatg gcggttcata tctttacatg ggccgctttt gttaatgttt ttagtttttg
    32281 tgtattcttt tgtgcct
//
LOCUS       DAZKIF010000046        32276 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZKIF010000046 DAZKIF010000000
VERSION     DAZKIF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.046"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(128..1888)
                     /gene="ycaO"
                     /locus_tag="ACHOTW_002110"
     CDS             complement(128..1888)
                     /gene="ycaO"
                     /locus_tag="ACHOTW_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP9844219.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            2018..2710
                     /locus_tag="ACHOTW_002111"
     CDS             2018..2710
                     /locus_tag="ACHOTW_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9844220.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2909..3997
                     /gene="serC"
                     /locus_tag="ACHOTW_002112"
     CDS             2909..3997
                     /gene="serC"
                     /locus_tag="ACHOTW_002112"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP9844221.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4068..5351
                     /gene="aroA"
                     /locus_tag="ACHOTW_002113"
     CDS             4068..5351
                     /gene="aroA"
                     /locus_tag="ACHOTW_002113"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9844222.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5520..6284
                     /gene="ycaL"
                     /locus_tag="ACHOTW_002114"
     CDS             5520..6284
                     /gene="ycaL"
                     /locus_tag="ACHOTW_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP9844223.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6457..7140
                     /gene="cmk"
                     /locus_tag="ACHOTW_002115"
     CDS             6457..7140
                     /gene="cmk"
                     /locus_tag="ACHOTW_002115"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9844224.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7251..8924
                     /gene="rpsA"
                     /locus_tag="ACHOTW_002116"
     CDS             7251..8924
                     /gene="rpsA"
                     /locus_tag="ACHOTW_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9844225.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9084..9368
                     /gene="ihfB"
                     /locus_tag="ACHOTW_002117"
     CDS             9084..9368
                     /gene="ihfB"
                     /locus_tag="ACHOTW_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP9844226.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9575..11839
                     /locus_tag="ACHOTW_002118"
     CDS             9575..11839
                     /locus_tag="ACHOTW_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP9844227.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11876..13624
                     /gene="msbA"
                     /locus_tag="ACHOTW_002119"
     CDS             11876..13624
                     /gene="msbA"
                     /locus_tag="ACHOTW_002119"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP9844228.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13621..14607
                     /gene="lpxK"
                     /locus_tag="ACHOTW_002120"
     CDS             13621..14607
                     /gene="lpxK"
                     /locus_tag="ACHOTW_002120"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP9844229.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14644..15876
                     /gene="ycaQ"
                     /locus_tag="ACHOTW_002121"
     CDS             14644..15876
                     /gene="ycaQ"
                     /locus_tag="ACHOTW_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP9844230.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15928..16110
                     /gene="ycaR"
                     /locus_tag="ACHOTW_002122"
     CDS             15928..16110
                     /gene="ycaR"
                     /locus_tag="ACHOTW_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP9844231.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16107..16853
                     /gene="kdsB"
                     /locus_tag="ACHOTW_002123"
     CDS             16107..16853
                     /gene="kdsB"
                     /locus_tag="ACHOTW_002123"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP9844232.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            17007..17900
                     /locus_tag="ACHOTW_002124"
     CDS             17007..17900
                     /locus_tag="ACHOTW_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP9844233.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17877..18656)
                     /gene="elyC"
                     /locus_tag="ACHOTW_002125"
     CDS             complement(17877..18656)
                     /gene="elyC"
                     /locus_tag="ACHOTW_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP9844234.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18792..19577
                     /gene="cmoM"
                     /locus_tag="ACHOTW_002126"
     CDS             18792..19577
                     /gene="cmoM"
                     /locus_tag="ACHOTW_002126"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP9844235.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19574..20896
                     /gene="mukF"
                     /locus_tag="ACHOTW_002127"
     CDS             19574..20896
                     /gene="mukF"
                     /locus_tag="ACHOTW_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP9844236.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20877..21581
                     /gene="mukE"
                     /locus_tag="ACHOTW_002128"
     CDS             20877..21581
                     /gene="mukE"
                     /locus_tag="ACHOTW_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP9844237.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21581..26041
                     /gene="mukB"
                     /locus_tag="ACHOTW_002129"
     CDS             21581..26041
                     /gene="mukB"
                     /locus_tag="ACHOTW_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP9844238.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26302..28149
                     /gene="ldtD"
                     /locus_tag="ACHOTW_002130"
     CDS             26302..28149
                     /gene="ldtD"
                     /locus_tag="ACHOTW_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9844239.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28330..28878
                     /locus_tag="ACHOTW_002131"
     CDS             28330..28878
                     /locus_tag="ACHOTW_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP9844240.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28905..29552
                     /gene="gloC"
                     /locus_tag="ACHOTW_002132"
     CDS             28905..29552
                     /gene="gloC"
                     /locus_tag="ACHOTW_002132"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP9844241.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29603..30793)
                     /gene="aspC"
                     /locus_tag="ACHOTW_002133"
     CDS             complement(29603..30793)
                     /gene="aspC"
                     /locus_tag="ACHOTW_002133"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP9844242.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30978..32066)
                     /gene="ompF"
                     /locus_tag="ACHOTW_002134"
     CDS             complement(30978..32066)
                     /gene="ompF"
                     /locus_tag="ACHOTW_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP9844243.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7875 a         7849 c         8660 g         7892 t
ORIGIN      
        1 cgatatttaa ccttataatt acaattattt tattaatgca aatatatgta aagcggggca
       61 ttaaaaaaac gccccgtaat ataactcaga cgaatcatta agcctacatt acgtaggcta
      121 tttgatttta ttttgcccag aatgctgctt tggcgcgctg cagcttttcg taggccttca
      181 acagcgactg atgtgcagca aacgcgtgca gatcgctatc tactggttgc aagccataaa
      241 acgccgcttc gccgctcatt gccgcactgg cggcttccac ggcatctgcg ccgtacatgc
      301 gaacaaacgc attcagatat tgcagcggct ggcgatcttc ttcctgcgcc agtaataaca
      361 acgtttgcag gcagcgataa tagttggcgc gttccgggct gaataccgat gagttaaact
      421 ccatcgtcca ttcggtccag accagagcct gttccagatc gccaccagcc agcgccagca
      481 tggcttttaa ttcaccaata cgcagggtgt accaaccgtt atccgacccg gtcgccagac
      541 ccaacagctc acgcacgcgg gtaaagtcat caaaaccttc ttcatccagt tgctcgatga
      601 ggttcaggta atcttctttt tcccactcgc tgcctggtag cgaaagaatc gtttcacgta
      661 aatggctgcc catactgtta ttcgcgagcc acagatcttc agccggataa atatcggaca
      721 tgccaggcac gataatacgg caagcataaa cgcccagatg ctcgtaatcg gcaatataaa
      781 cttctttatc ttctttgttg aagatagcca tcagcgtagc aaactcttct tccgtggtgc
      841 cggagaaatt ccagtccaca aacggataat cggcatcctg cttgaacagg tcccaggaga
      901 ttaaaccgct ggaatcgata aagtgcgttt cgaggttggt atgttcagcg acttcttcat
      961 catcgaaggt tggcggagta aacacatcca aatctttcag gccacgacct tgcagcagct
     1021 cggtcacggt acgttccagt gctacgccaa aatcaggatg cgcaccgaaa gaggcaaagc
     1081 aggtgccgtt agcaggatta aacagtacca cgcaaatcac cggatactgg ccgccaaggg
     1141 aaccatcata tgcgaagatc gggaaacctt ctgcttccag tgtttcgatc gcttcaacca
     1201 ccgctgggta acgcgccagc acgtctgccg ggatttccgg caagctgatg ctttcagcaa
     1261 taatgcggtt tttcacgtag cgttcgaaaa cttcggacaa cccctgaacg cgtgcttcgt
     1321 tacgggtatt gccagcagac ataccgttag aaacgtacag gttaccaatg atattcatcg
     1381 gaatataaac ggtctgatta tcagactgac gtgtaaacgg cagaccgcaa ataccacgat
     1441 cttcgttacc ggattgtagg tcaatcagca tactgccggt caattcattc tccggatcgt
     1501 aaaatgcgcg cagacgttca tcgagcagcc cttctggcac atcgtcattt tcggtcagtg
     1561 ggaaccattt ttcgttggga taatgcacaa atggaccgtt ggcgatggtt tcgcccagcc
     1621 agaaatcagc aaaaaagtag ttggttgaga gacgctcgaa atattcaccg agtgcagaag
     1681 ccagcgccgc tttcttggtt gcgcctttac cgttggtaaa acacagtgcg cactctttgt
     1741 cgcgaatatg tacagaccag acgttaggca cgggattcag ccaggaggcc tcttcaatct
     1801 gaaagccgag gtctgaaagt ttttgctgga agcgagcgat ggaatcttcc agtgcggcat
     1861 ctttgccggg gataaatgtt tgcgtcatga aaatcacttt agtcgtacgg aaagcgcgca
     1921 ataatacggg ttttatctca aaggcgctat caccgccgcc atgccgatga actgttgact
     1981 atgcttttag cggataaacc acgttaagag cataaaaatg aaggcgttcg atctccaccg
     2041 tatggcattt gataaagtgc cttttgattt ccttggcgaa gttgcactac gtagtcttta
     2101 tacctttgta ctggtctttt tgttcctcaa aatgaccgga agacgcggtg tgcggcagat
     2161 gtcgctgttt gaagttttaa tcattctgac gctgggatca gcggcgggag atgtggcgtt
     2221 ttatgatgat gtgccgatgg tcccggtact tatcgtcttt attactctgg cgttgttata
     2281 ccgcctggta atgtggttga tggcgcacag tgaaaaactg gaagatcttc tggaaggcaa
     2341 gccagttgtc attattgaag atggcgagct ggcctggtcg aaactcaata actccaacat
     2401 gacggaattt gagttcttta tggagctacg attgcgtggc gtggagcagc tggggcaggt
     2461 acgtctggcg attctcgaaa ccaacgggca aatcagtgtc tatttctttg aagatgacaa
     2521 ggtgaaatcg ggtttactta ttttacccag tgattgtacg cagcgttaca aagtggtgcc
     2581 ggagtcggcg gactatgcct gtattcgttg tagtgaaatc attcatatga aagcggggga
     2641 aaaacaatta tgtccgcgct gtgcaaatcc agaatggacg aaggcaagtc gggcaaaacg
     2701 ggtgacctga cagtaaaaac atcggctttt tgctaataat ccgagagatt cttttgtgtg
     2761 atgcaagcca catttttgcc ctcaacggtt ttactcattg cgatgtgtgt cactgaatga
     2821 taaaaccgat agccacagga ataatgtatt acctgtggtc gcaatcgatt gaccgcgggt
     2881 taatagcaac gcaacgtggt gaggggaaat ggctcaaatc ttcaatttta gttctggtcc
     2941 ggcaatgcta ccggtagagg tgcttaaaca ggctcaacag gaactgcgcg actggaacgg
     3001 tcttggtacg tcggtgatgg aagtgagtca ccgtggcaaa gagttcattc aggttgcaga
     3061 ggaagccgag aaggattttc gcgatcttct taatgtcccc tccaactaca aggtattatt
     3121 ctgccatggc ggtggtcgcg gtcagtttgc tgcggtaccg ctgaatattc tcggtgataa
     3181 aaccaccgca gattatgttg atgccggtta ctgggcggca agtgccatta aagaagcgaa
     3241 aaaatactgc acgcctaatg tctttgacgc caaagtgact gttgatggtc tgcgcgcggt
     3301 taagccaatg cgtgaatggc aactctctga taatgctgct tatatgcatt actgcccgaa
     3361 tgaaaccatc gacggtatcg ccatcgacga aacgccagac ttcggcaaag atgtggtggt
     3421 cgccgccgac ttctcttcaa ccattctttc ccgtccgatt gacgtcagcc gttatggtgt
     3481 aatttacgct ggcgcgcaga aaaatatcgg cccggcaggc ctgacaatcg tcatcgtccg
     3541 tgaagatttg ctgggcaaag cgaatgtcgc gtgtccgtcg attctggatt attccatcct
     3601 caacgataac ggctccatgt ttaacacgcc gccgacattt gcctggtatc tatctggtct
     3661 ggtctttaaa tggctgaaag cgaacggcgg tgtagctgaa atggataaaa tcaatcagca
     3721 aaaagcagaa ctgctatatg gggtgattga taacagcgat ttctaccgca atgacgtggc
     3781 gaaagctaac cgttcgcgga tgaacgtgcc gttccagttg gcggacagtg cgcttgacaa
     3841 attgttcctt gaagagtctt ttgctgcagg ccttcatgca ctgaaaggtc accgtgtggt
     3901 cggcggaatg cgcgcgtcta tttataatgc catgccgctg gaaggcgtta aagcgctgac
     3961 agacttcatg gttgagttcg aacgccgtca cggttaatgc cgaaattttg cttaatcccc
     4021 acagccagcc tgtggggttt ttatttctgt tgtagagagt tgagttcatg gaatccctga
     4081 cgttacaacc catcgctcgt gtcgatggca ctattaatct gcccggttcc aagagcgttt
     4141 ctaaccgcgc tttattgctg gcggcattag cacacggcaa aacagtatta accaatctgc
     4201 tggatagcga tgacgtgcgc catatgctga atgcattaac agcgttaggg gtaagctata
     4261 cgctttcagc cgatcgtaca cgttgcgaaa ttatcggtaa cggcggtcca ttacacgcag
     4321 aaggtgccct ggagttgttc ctcggtaacg ccggaacggc aatgcgtccg ctggcggcag
     4381 ctctttgtct gggtagcaat gatattgtgc tgaccggtga gccgcgtatg aaagagcgcc
     4441 cgattggtca tctggtggat gcgctgcgcc tgggcggggc gaagatcact tacctggaac
     4501 aagaaaatta tccgccgttg cgtttacagg gcggctttac tggcggcaac gttgacgttg
     4561 atggctccgt ttccagccaa ttcctcaccg cactgttaat gactgcgcct cttgcgccgg
     4621 aagatacggt gattcgtatt aaaggcgatc tggtttctaa accttatatc gacatcacac
     4681 tcaatctgat gaagacgttt ggtgttgaaa ttgaaaatca gcactatcaa caatttgtcg
     4741 taaaaggcgg gcagtcttat cagtctccgg gtacttattt ggtcgaaggc gatgcatctt
     4801 cggcttctta ctttctggca gcagcagcaa tcaaaggcgg cactgtaaaa gtgaccggta
     4861 ttggacgtaa cagtatgcag ggtgatattc gctttgctga tgtgctggaa aaaatgggcg
     4921 cgaccatttg ctggggcgat gattatattt cctgcacgcg tggtgaactg aacgctattg
     4981 atatggatat gaaccatatt cctgatgcgg cgatgaccat tgctacggcg gcgttatttg
     5041 caaaaggcac caccacgctg cgcaatatct ataactggcg tgttaaagaa accgatcgcc
     5101 tgtttgcgat ggcaacagaa ctgcgtaaag tcggtgcgga agtagaagag gggcacgatt
     5161 acattcgtat cactccaccg gaaaaactga attttgccga gatcgcgaca tacaatgatc
     5221 accggatggc gatgtgtttc tcgctggtgg cgttgtcaga tacaccagtg acgattcttg
     5281 atcccaaatg tacggccaaa acatttccgg attatttcga gcagctggcg cggattagcc
     5341 aggcagcctg aatgaataac gtccaataaa tagccaaaac tttctttatc aaaacgtcgg
     5401 cacattgccg gcgttttttt tcggaccttg tgagtcattt tgattaatgg tagcgtcgct
     5461 tgtcaatgta agttgttgat acataatgtt tatatatgat taatcaacgg atgattcaca
     5521 tgaagaatac taaattactg ctggcgattg tgacctctgc agcattactg acggggtgtc
     5581 aaaataccca cggtattgat accaatatgg ctatcagctc cggtttaaat gcctataaag
     5641 cagcaacgtt aagcgatgcc gatgcaaaag cgattgccaa tcagggctgt gccgaaatgg
     5701 acagcggcaa tcaagtcgca agtaaatcca gcaagtacgg taaacgtctg gcaaaaatcg
     5761 ccaaagcatt gggtaacaat attaatggca cgccggtcaa ctataaggtt tatatgacca
     5821 gcgacgtcaa cgcatgggcg atggcgaacg gctgtgttcg tgtttacagt ggcctgatgg
     5881 acatgatgaa cgataacgaa attgaaggcg ttctgggcca tgaactgggc cacgtcgcgt
     5941 tgggtcactc gctggctgaa atgaaagctt cttatgcgat cgttgccgca cgcgatgcca
     6001 tttcagctac cagcggtgtg gcttcccagc tttcccgctc acaattgggt gatatcgcag
     6061 aaggcgctat caatgcgaaa tactcccgcg ataaagagtc cgaagcagat gatttctcct
     6121 ttgatctgtt gaagaaacgt ggcatcagca cccaggggct ggttggcagc tttgaaaaac
     6181 tggctagcct ggatggcggt cgcacccagt ccatgtttga ctctcaccca ccatcaacag
     6241 agcgtgcgca acacatccgt gatcgtatcg cctctggtaa gtaaatcatt gtcatctttc
     6301 gggctggtct tctgccagcc cgctataatt gcgcaataaa tccccatctg aatacagaca
     6361 aaactggttt ttgcacacaa cgttaacgat ttgtggcgtc ggcgcgtata atgcgcgcgg
     6421 ttatgttaac ggtacgcctg ttttaaggag ataaagatga cggcaattgc cccggttatt
     6481 accattgatg gcccaagcgg tgcagggaaa ggcaccttgt gtaaggctat ggcggaagcg
     6541 ttgcaatggc atctgctgga ttcgggtgca atttatcgcg tactggcact agcggcatta
     6601 catcaccatg ttgatgttgc atcggaagat gcgctggtgc cgctggcatc ccatctggat
     6661 gtacgttttg tgtcaaccaa tggcaatctg gaagtgatcc tcgaagggga agatgtcagc
     6721 ggcgaaattc gtactcagga agtggcgaat gcagcttcac aagtcgcggc attcccacgc
     6781 gttcgtgaag cattattgcg tcgccaacgc gcgtttcgcg aattaccagg cctgattgcc
     6841 gatggccgcg acatgggaac ggtggtattc cctgatgcgc cagtgaaaat tttccttgac
     6901 gcctcctcgg aagaacgtgc gcatcgccgc atgctacagt tgcaggagaa gggctttagt
     6961 gttaactttg agcgcctttt ggccgagatc aaagaacgcg acgaccgcga tcgtaaccga
     7021 gcggtagcgc cactggttcc ggcagccgat gctttagtgt tggattccac caccttaagc
     7081 attgagcaag tgattgaaaa agcgctacaa tacgcgcgcc agaaattggc tctcgcataa
     7141 gcgaccgaat ttgcagtacc cccgttgcaa tggaatgaca gcgggtatgt taaacaaccc
     7201 catccggcat ggagccaggt ggacgttaaa tataaacctg aagattaaac atgactgaat
     7261 cttttgctca actctttgaa gagtccttaa aagaaatcga aacccgcccg ggttctatcg
     7321 ttcgtggcgt tgttgttgct atcgacaaag acgtagtact ggttgacgct ggtctgaaat
     7381 ctgagtccgc catcccggct gagcagttca aaaacgccca gggcgagctg gaaatccagg
     7441 taggtgacga agttgacgtt gctctggacg cagtagaaga cggcttcggt gaaactctgc
     7501 tgtcccgtga gaaagctaaa cgtcacgaag cctggatcac gctggaaaaa gcttacgaag
     7561 atgctgaaac tgttaccggt gttatcaacg gcaaagttaa gggcggcttc actgttgagc
     7621 tgaacggtat tcgtgcgttc ctgccaggtt ctctggtaga cgttcgtccg gtgcgtgaca
     7681 ctctgcacct ggaaggcaaa gagcttgaat tcaaagtaat caagctggat cagaagcgca
     7741 acaacgttgt tgtttctcgt cgtgccgtta tcgaatccga aaacagcgca gagcgcgatc
     7801 agctgctgga aaacctgcag gaaggcatgg aagttaaagg tatcgttaag aacctcactg
     7861 actacggtgc attcgttgat ctgggcggcg ttgacggcct gctgcacatc actgacatgg
     7921 cctggaaacg cgttaagcat ccgagcgaaa tcgtcaacgt gggcgacgaa atcactgtta
     7981 aagtgctgaa gttcgaccgc gaacgtaccc gtgtatccct gggcctgaaa cagctgggcg
     8041 aagatccgtg ggtagctatc gctaaacgtt atccggaagg taccaaactg actggtcgcg
     8101 tgaccaacct gaccgactac ggctgcttcg ttgaaatcga agaaggcgtt gaaggcctgg
     8161 tacacgtttc cgaaatggat tggaccaaca aaaacatcca cccgtccaaa gttgttaacg
     8221 ttggcgatgt agtggaagtt atggttctgg atatcgacga agaacgtcgt cgtatctccc
     8281 tgggtctgaa acagtgcaaa gctaacccgt ggcagcagtt cgcggaaacc cacaacaagg
     8341 gcgaccgtgt tgaaggtaaa atcaagtcta tcactgactt cggtatcttc atcggcctgg
     8401 acggcggcat cgacggcctg gttcacctgt ctgacatctc ctggaacgtt gcaggcgaag
     8461 aagcagttcg tgaatacaaa aaaggcgacg aaatcgctgc agttgttctg caggttgacg
     8521 cagaacgtga acgtatctcc ctgggcgtta aacagctcgc agaagatccg ttcaacaact
     8581 gggttgctct gaacaagaaa ggcgctatcg taaccggtaa agtaactgca gttgacgcta
     8641 aaggcgcaac cgtagaactg gctgacggcg ttgaaggtta cctgcgtgct tctgaagcat
     8701 cccgtgaccg cgttgaagac gctaccctgg ttctgagcgt tggcgacgaa gttgaagcta
     8761 aattcaccgg cgttgatcgt aaaaaccgtg caatcagcct gtctgttcgt gcgaaagacg
     8821 aagctgacga gaaagatgca atcgcaactg ttaacaaaca ggaagatgca aacttctcca
     8881 acaacgcaat ggctgaagct ttcaaagcag ctaaaggcga gtaattctct gactcttcgg
     8941 gatttttatt ctgaagtttg ttgagtttac ttgacagatt gcaggtttcg tcctgtaatc
     9001 aagcactaag ggcggctacg gccgccctta atcaatgcag caacagcagc cgcttaattt
     9061 gcctttaagg aaccggagga atcatgacca agtcagaatt gatagaaaga cttgccaccc
     9121 agcaatcgca cattcccgcc aagacggttg aagatgcagt aaaagagatg ctggagcata
     9181 tggcctcgac tcttgcgcag ggcgagcgta ttgaaatccg cggtttcggc agtttctctt
     9241 tgcactaccg cgcaccacgt accggacgta atccgaagac tggcgataaa gtagaactgg
     9301 aaggaaaata cgttcctcac tttaaacctg gtaaagaact gcgcgatcgc gccaatattt
     9361 acggttaagt ttttactcaa acttgaacga gagaaaagca cctgtcgggt gcttttttca
     9421 tttatctaat ctggaactgg aagctgcctc gcagagtttt gagcagtttt caccctttcg
     9481 ttaaattctt ctgaatgtgc ctcggggaac gcaaaattcc caccagacaa ccgctcaaca
     9541 aagttgcaca ctttccataa acagggaggg gtgcatgaaa ataacgacag tcggcgtatg
     9601 cataatttgc ggaatttttc cgttgctgat tttgccccaa ttgcctggga cattaaccct
     9661 tgcgtttctg actctcttcg cctgcgtact ggcatttatc cctgttaaaa ccgtccgtta
     9721 tatcgcgctg acgttgctgt ttttcgtttg gggcatatta tcagcaaagc aaattttgtt
     9781 ggcaggagaa accttaactg gcgcgacgca ggatgcaatt gttgagatca ctgctactga
     9841 cggcatgacc actcattacg gtcaaattac ttatctacaa ggtcaacgta tattccctgc
     9901 gccaggcctt gtgctgtatg gcgaatatct tccgcaagcg gtttgtgccg gacaactgtg
     9961 gtcaatgaaa ctcaaagttc gtgccgttca tggtcaactt aatgatggcg gctttgatag
    10021 ccagcgttat gccattgccc agcatcagcc gctcaccggc cgctttctgc aggcaagtgt
    10081 tattgaaccg aattgtagcc tgcgtgcaca gtatctggcg tcactacaaa caacgctgca
    10141 accctatccg tggaatgcgg ttattcttgg tttaggtatg ggggaacggt tatccgtccc
    10201 taaagaaatc aaaaatatca tgcgtgatac tggaacggcg catttaatgg cgatatcggg
    10261 attgcacatc gcttttgcgg cgttgctggc tgccggactc attcgcagtg gacaaatttt
    10321 tctgcctggg cgctggatcc actggcaaat gccattaatt ggcggaatct gctgtgctgc
    10381 tttttatgcc tggctgacgg gaatgcaacc tcctgcattg cgtaccgttg tggcgcttgc
    10441 tacgtggggt atgcttaagt taagtgggcg acagtggagt ggctgggatg tatggatatg
    10501 ttgtctggcg gcaattttgc tgatggatcc tgttgtcatt ctctcgcaaa gtttatggct
    10561 ctctgccgct gcggtcgcgg cattgatatt ttggtatcag tggtttccct gtcctgagtg
    10621 gcaactgccg ccggtattgc gtgcagttgt ttccctcatc catctgcaac tgggaatcac
    10681 acttctgctt atgcccgtgc aaatcgtcat ttttcatggc attagtctga cctcgtttat
    10741 tgcaaatcta ttagcaattc ccttggtgac atttatcacg gttccgttga tcctcgccgc
    10801 gatggttgtg catttaagcg ggccgttaat cctggatcaa gggttatggt ttcttgccga
    10861 ccggtctttg gctttacttt tcttggggtt aaagagtttg ccagaagggt ggatcaacat
    10921 tgctgaacgt tggcaatggc tatcattttc cccatggttc ttactggtgg tatggcgatt
    10981 aaacgcctgg cgaacgttgc cagcaatgtg tgtggctgta ggcttgctga tgtgctggcc
    11041 gctgtggcaa aaacctcgac ctgacgagtg gcaagtgtac atgcttgatg tcgggcaagg
    11101 gctggcaatg gtgatagcca gaaacggcaa agcgattctc tatgacacgg gactggcctg
    11161 gcctgaaggg gatagtgggc aacaactgat tatcccctgg ctccactggc ataatcttga
    11221 accggaaggc gttatcctga gtcatgaaca tctggatcac cggggagggc tggactcaat
    11281 attgcataca tggccgatgt tatggatcag aagtccgtta aactgggaac atcatcagcc
    11341 ctgtgtgcgt ggcgaagcgt ggcaatggca aggattgcgt ttcagcgcgc actggccttt
    11401 acaaggtcgc aacgataaag gaaataacca ttcctgtgtg gttaaggttg atgacgggac
    11461 gaatagcatt cttctaaccg gtgatattga agccccagct gaacaaaaga tgctaagccg
    11521 ttactggcag caagtgcagg caacattgct tcaggtacct caccatggca gtaatacctc
    11581 atcatcattg ccattaattc agcgagtgaa tggaaaagtg gcactcgcat cggcatcgcg
    11641 ctataacgca tggcgactgc cctctaacaa agttaagcat cgctatcaac aacaaggtta
    11701 tacgtggctt gatactcctc atcaggggca agtaacggtc gatttttcag cgcaaggctg
    11761 gcggattagc agcctcaggg agcaaatttt acctcgttgg tatcatcagt ggtttggcgt
    11821 gccagtggat aacgggtaga atatgcggct atttcaacaa atgctggttt tttgaatgca
    11881 taacgacaaa gatctctcta cgtggcagac attccgccga ctgtggccaa ccattgcgcc
    11941 ttttaaagcg ggtctgatcg tggcgggcgt agcgttaatc ctcaacgcag ccagcgatac
    12001 cttcatgtta tcgctcctta agccacttct tgatgatggc tttggtaaaa cagatcgctc
    12061 cgtgctggtg tggatgccgc tggtggtgat cgggctgatg attttgcgtg gtatcaccag
    12121 ctatgtctcc agctactgta tctcctgggt atcaggaaag gtggtaatga ccatgcgtcg
    12181 ccgcctgttt ggtcacatga tgggaatgcc ggtttcattc tttgacaaac agtcaacggg
    12241 tacgctgttg tcacgtatca cctacgattc cgaacaggtt gcttcttcct cttccggcgc
    12301 actgattact gttgtgcgtg aaggtgcgtc gatcatcggc ctgttcatca tgatgttcta
    12361 ttacagttgg caattgtcga tcattttgat tgtgctggca ccgattgttt cgattgcgat
    12421 tcgcgtagta tcgaagcgtt ttcgcaacat cagtaaaaac atgcagaaca ccatggggca
    12481 ggtgaccacc agcgctgaac aaatgctgaa aggccataaa gaagtattga ttttcggtgg
    12541 tcaggaagtg gaaacgaaac gcttcgataa agtcagcaac cgaatgcgtc ttcaggggat
    12601 gaaaatggtt tcagcctctt ccatctctga tccgatcatt cagctgatcg cctctttggc
    12661 gctggcgttt gttctgtatg cggcgagctt cccaagtgtc atggatagcc tgactgccgg
    12721 tacgattacc gttgttttct cttcaatgat tgcactgatg cgtccgctga aatcgctgac
    12781 taacgttaac gcccagttcc agcgcggtat ggcggcttgt cagacactgt ttaccattct
    12841 ggacagtgag caggagaaag acgaaggtaa gcgcgtgatc gagcgtgcga ctggcgacgt
    12901 ggaattccgc aacgtcacct ttacttatcc gggacgtgac gtacccgcat tgcgtaacat
    12961 caacctgaaa attccggcag ggaaaacggt cgctctggtt ggacgctctg gttcaggtaa
    13021 atcaaccatc gccagcctga tcacgcgttt ttacgatatt gatgaaggcg aaatcctgat
    13081 ggatggtcac gatctgcgcg agtataccct ggcgtcgttg cgtaaccagg ttgctctggt
    13141 gtcgcagaat gtccatctgt ttaacgatac ggttgctaac aacattgctt acgcacgaac
    13201 tgaacagtac agccgtgagc aaattgaaga agcggcgcgt atggcctacg ccatggactt
    13261 catcaataag atggataacg gtctcgatac agtgattggt gaaaacggcg tactgctctc
    13321 tggcggtcag cgtcagcgta ttgctatcgc tcgagccttg ttgcgtgata gcccgattct
    13381 gattctggat gaagctacct cggctctgga taccgaatcc gaacgtgcga ttcaggcggc
    13441 actggatgag ttgcagaaaa accgtacctc tctggtgatt gcccaccgct tgtctaccat
    13501 tgaaaaggca gacgaaatcg tggtcgtcga ggatggtgtc attgtggaac gcggtacgca
    13561 taacgatttg cttgagcacc gcggcgttta cgcgcaactt cacaaaatgc agtttggcca
    13621 atgatcgaaa aaatctggtc tggtgaatcc cctttgtggc ggctattgct gccactctcc
    13681 tggttgtatg gcctggtgag tggcgcgatc cgtctttgct ataaactaaa actgaagcgc
    13741 gcctggcgtg cccccgtacc ggttgtcgtg gttggtaatc tcaccgcagg cggcaacgga
    13801 aaaaccccgg tcgttgtctg gctggtggaa cagttgcaac agcgcggtat tcgcgtgggg
    13861 gtcgtatcgc ggggatatgg tggtaaggct gaatcttatc cgctgttatt gtcggcagat
    13921 accaccacag cacaggcggg tgatgaacct gtgttgattt atcaacgcac tgatgcgcct
    13981 gttgcggttt ctcccgttcg ttctgatgcg gtaaaagcca ttctggcgca acaccctgat
    14041 gtgcagatca tcgtaaccga cgacggttta cagcattacc gtctggcgcg tgatgtggaa
    14101 attgtcgtta ttgatggtgt gcgtcgcttt ggcaatggct ggtggttgcc ggcggggcca
    14161 atgcgtgagc gagcggggcg cttaaagtca gttgatgcgg taatcgtcaa cggcggtgtc
    14221 ccccgcagcg gtgaaatccc catgcatctg ctgccgggtc aggcggtgaa tttacgtacc
    14281 ggtacgcgtt gtgacgttgc tcagcttgaa catgtggtgg cgatggcagg gattgggcat
    14341 ccgccgcgct tttttgccac gctgaagatg tgcggcgtac aaccggaaaa atgtgtaccg
    14401 ctggccgatc atcagtcttt gaaccatgcg gatgtcagcg cgttggtaag caccgggcaa
    14461 acgctggtaa tgactgaaaa agatgcggtg aaatgccggg cctttgcaga agaaaattgg
    14521 tggtatttgc ccgttgacgc acagctttca ggtgatgaac cagcgaaact gcttgcgcaa
    14581 ctaacctcgc tggcttctgg caactagtta cgccgcggca gcgttcgatt gatggagtca
    14641 tgaatgtcgc tgccgcacct ctcccttgct gatgcgcgta atcttcacct tgccgcgcaa
    14701 ggcctgttaa acaaaccccg ccgtcgagcg tcgttggagg atattccggc aacgatctcc
    14761 cgcatgtcct tgctgcaaat cgataccatc aatattgttg cccgtagccc atatctggtg
    14821 cttttcagtc gtctgggaaa ttatcctgcc cagtggctgg atgagtctct ggcgcgtggc
    14881 gaattaatgg aatactgggc gcatgaagcc tgctttatgc cacgcagtga ctttcgtctt
    14941 attcgccacc gcatgctggc acctgaaaaa atgggctgga aatacaaaga cgcctggatg
    15001 caggaacatg aggcggaaat tgcacagtta attcagcata ttcatgataa ggggccggta
    15061 cgttcagccg attttgagta tcctcgtaaa ggtgcaagcg gctggtggga atggaagccg
    15121 cataaacggc atctggaagg tttatttact gccggaaagg tgatggtgat tgaacggcgc
    15181 aacttccagc gcgtttatga tttaacccac cgtgtcatgc ctgactggga tgatgagcgc
    15241 gatctcgttt cgcaaacaga agcagaaatc atcatgctgg ataacagtgc gcgtagccag
    15301 ggaatattcc gcgaacagtg gctggcagat tactatcggc tgaaacgtcc ggcactggca
    15361 gcgtggcgcg aagcgagggc tgaacagcgg caaatcattg ctgtgcatgt tgaaaaattg
    15421 ggcaatcttt ggctgcatgc tgatttgctg ccgctactcg agcgagcgct ggccggaaag
    15481 ctcactgcaa cgcacagcgc ggtactttcg ccttttgatc ctgttgtctg ggatcgcaaa
    15541 cgcgcagagc agctttttga ttttagctac cggctggagt gctatacccc agcgccgaaa
    15601 cgccagtatg gctattttgt tctgccgtta ttacatcgtg ggcaattagt tgggcgaatg
    15661 gatgccaaaa tgcatcgcca gacaggcatc cttgaagtta tctctctgtg gttacaggaa
    15721 ggtattaaac caacgacaac gctgcaaaaa gggttacgtc aggcgattac tgatttcgct
    15781 aactggcagc aggcaacgcg ggtgacatta ggacactgcc cgcaaggtct ctttacggat
    15841 tgccgcaccg gctgggaaat agaccccgtc gcataaatga ttatgataag atttatgcat
    15901 tcataacccg atccatctgg aggaactatg gatcatcgtc tgcttgaaat cattgcctgc
    15961 ccggtttgca acggaaaact ttggtataac caggaaaaac aagaacttat ttgcaaactg
    16021 gacaacctgg ctttcccgct gcgcgatggc attccagtgt tgctggaaac ggaagcccgc
    16081 gtgctgactg ctgatgagag taaatcatga gttttgtggt cattattccc gcgcgctacg
    16141 cgtccacgcg tctgcccggt aaaccattgg ttgatattaa cggcaaaccc atgattgttc
    16201 atgttcttga acgcgcgcgc gaatcaggtg ccgagcgcat catcgtggca accgatcatg
    16261 aggatgtcgc ccgcgccgtt gaagccgctg gcggtgaagt atgtatgacg cgcgccgatc
    16321 accagtcagg gacagaacgc ttggcggaag ttgtcgaaaa atgcgcgttc agcgacgaca
    16381 cggtgatcgt taatgtgcag ggtgatgaac cgatgatccc tgcgacaatc attcgtcagg
    16441 ttgctgataa cctcgctcag cgtcaggtgg gtatggcgac tctggcggtg ccaatccaca
    16501 atgcggaaga agcgtttaac ccgaatgcgg tgaaagtggt tctcgacgct gaagggtatg
    16561 cactgtactt ctcacgcgct accattccgt gggatcgtga tcgttttgca aaaggccttg
    16621 aaaccgttgg cgataacttc ctgcgtcatc ttggtattta tggctaccgt gcaggcttta
    16681 tccgtcgtta cgtcacctgg cagccaagcc cgttagaaca catcgaaatg ttagagcagc
    16741 ttcgtgtttt gtggtacggc gaaaaaatcc atgttgctgt cgcgcatgaa gttcctggca
    16801 caggtgtgga tacccctgaa gatctcgagc gcgttcgcgc tgaaatgcgc taatttcact
    16861 tcacgacact tcagccaatt ttgggaggag tgtcgtaccg ttacgatttt cctcaatttt
    16921 tcttttcaac aattgatctc attcaggtga catcttttat attggcgctc attatgaaag
    16981 cagtagcttt tatgagggta atctgaatgg aacagctgcg tgccgaatta agccatttac
    17041 tgggcgaaaa actcagtcgt attgagtgcg tcaatgaaaa agcggatacg gcgttgtggg
    17101 ctttgtatga cagccaggga aacccaatgc cgctaatggc aagaagcttt agtacgcccg
    17161 gaaaagcccg acaactggca tggaaaacca ccatgctggc aagaagtggg actgtccgta
    17221 tgccgactat ttatggtgtg atgacgcatg aagaacaccc cggccctgat gtcctgctac
    17281 tggagcggat gcgtggtgtt tcggtagagg caccagcccg aacaccagaa cgttgggaac
    17341 agctcaaaga ccaaatcgtt gaagccttac tggcctggca ccgtcaggac agtcgcggtt
    17401 gcgtcggcgc ggtcgacaat actcaggaaa atttctggcc ctcatggtac cggcaacatg
    17461 ttgaagtgct atggaccacg ctcaatcagt tcaataacac cggtctgacg atgcaggata
    17521 agcggatcct gtttcgcact cgcgaatgtc tcccggcatt atttgaaggt tttaacgaca
    17581 attgtgtgct gattcacggt aacttctgtt tacgcagcat gttgaaagat tcgcgcagcg
    17641 atcagttact ggcgatggtc gggccgggac taatgctttg ggcaccgcga gaatacgaac
    17701 tgttccgact aatggataat tctctggcgg aagatttgct ctggagttac ctgcaacgcg
    17761 cgccagtggc ggagtcgttc atctggcggc gttggttgta tgtgttatgg gatgaagttg
    17821 cgcaactggt taataccgga cgatttagtc ggcgcaactt cgatctggca tcaaaatcac
    17881 tcttgccgtg gctcgcctga cgaacctttt agccactgcc agatgcggcc aagcgtttca
    17941 tagccaacac gatcgctatg catcaaccag accggagaag ggattgcccg ttcccacggg
    18001 ttaagcggcg agtcgatggc caactgattc gccggggctg gtagtggatt taacccttcc
    18061 tgctgaaaaa agatcatcgc ccgcggcaga tgtgatgcgg aggtcaccag caggaaaggg
    18121 gcgtcaccaa tcgcctgttt cactgccgca gcttcttctt cggtatcttt tggcaaatcc
    18181 agggtgataa tttgctcgcg cggtacgccc agcgattgcg caactctggc acctacttcc
    18241 gctgtactca ccgtattggt ttttgctacg cctcccgtga agatcagttt tgaacccgga
    18301 ttttcgcgcc ataaacggat accctcattc aggcgtggca gactgttatt gattaaatta
    18361 gagctcggtg cccactgcgg gttccaggta taaccacctc cgagcaccac gatatagtcc
    18421 actttttgcg aattattcca tgtcggatag gtgctttcga tgggacgcag caggcggtcg
    18481 gctaccggtt gcaaactcaa taacaagagt gccagccacc caatgctaat aaaaatcttc
    18541 ccggtttttt gaaaacggct aaaccacagt agcgccaggc cagcgccaat aattaacaac
    18601 atcagcggaa gtggcagcaa catattgcca atcacttttt tcagtgtaaa aagcatcctt
    18661 tctggttcct tttttaacca tatagcaagg gatcgccagc gatattacac cagacgggtt
    18721 cattctcgcc gtggctgtga caaaatagcg tctttatcag aaaacgtctt agccaaccgg
    18781 tggagaaagc aatgcaggat cgcaattttg atgatattgc ggaaaagttt tcccgtaaca
    18841 tttacggcac caccaaaggg cagcttcgac aggctattct gtggcaggat ctcgatcgcg
    18901 tgctggcgga aatgggtccg caaaaactgc gtgtgctgga tgcgggcggt ggagaagggc
    18961 agaccgcaat caaaatggcc gagcgcgggc atcaggtcat tttatgcgat ctttctgcgc
    19021 agatgatcga ccgcgcaaaa caggcggcag aagcaaaagg tgtgagcgac aacatgcaat
    19081 ttatacattg cgccgctcag gatgttgctt cgcatttgga aacgcccgtt gatctgatat
    19141 tgttccatgc ggtgctcgag tgggtggctg atccccgcag cgtattgcag accctgtggt
    19201 cagtgttgcg tccaggcggc gtgttgtcgt taatgttcta caatgcgcat ggtttgttga
    19261 tgcataacat ggtcgccggg aattttgatt acgtgcaggc gggaatgccg aaaaagaaaa
    19321 aacggacgct ttcgccagat tatccacgcg acccgacgca ggtttatctg tggctggaag
    19381 aagctggttg gcaaattatg ggtaagacag gcgttcgcgt gtttcatgat tatctgcgcg
    19441 agaagcacca gcagcgcgac tgctatgaag cattacttga attagaaacg cgttattgcc
    19501 gtcaggaacc gtatattacc ctggggcgtt atattcatgt caccgcgcgc aaaccgcaga
    19561 gcaaggataa agtatgagtg aattttccca gacagtcccc gaactggttg cctgggccag
    19621 aaaaaatgac ttctccattt cgctgccggt agaccgactc tcttttctgc tggcagttgc
    19681 cacgctgaac ggcgagcgtc tggatggtga gatgagtgaa ggcgagctgg tggatgcatt
    19741 ccgccatgtg agtgatgcgt ttgagcaaac cagcgaaacc atcggcgtgc gcgccaacaa
    19801 cgcgatcaac gacatggtgc gtcaacgtct gctgaaccgc tttaccagcg agcaggcaga
    19861 agggaacgca atttaccgtc tgacgccgct cggcatcggc attactgact attacatccg
    19921 tcagcgcgag ttttctacgc tgcgcctttc tatgcagttg tcgattgtgg cgggtgagct
    19981 caaacgcgca gcagatgccg ccgaagaggg cggtgatgaa tttcactggc accgtaatgt
    20041 ctatgcgcca ctgaaatatt cggtagcaga aattttcgac agtatcgacc tgacgcaacg
    20101 tctgatggac gaacagcagc agcaggtgaa ggacgatatc gcccagttgc tgaacaaaga
    20161 ctggcgggcg gcgatttcca gctgtgaatt gttgctttcg gaaacttccg gaacgctgcg
    20221 tgaattgcag gatacactgg aagcggcagg cgacaaattg caggctaatc tgttgcgcat
    20281 tcaggatgcg acgatgactc atgacgatct gcatttcgtc gatcgtctgg tgttcgatct
    20341 gcagagcaaa ctcgaccgta ttatcagttg gggccagcaa tccatcgatt tgtggattgg
    20401 ctacgaccgc cacgtacata aatttattcg taccgcgatc gatatggata aaaaccgcgt
    20461 ctttgctcag cggttacgtc agtcggtaca aacctatttt gatgagccgt gggcgctaag
    20521 ttatgccaat gccgatcgtc tgctggatat gcgtgacgaa gagatggcac tgcgcgatga
    20581 agaagtgact ggggaacttc cgccagattt ggaatacgaa gagtttaacg agatccgcga
    20641 acagctggcg gcgatcatcg aagaacaact tgccgtgtac aaaaccagac aagtgccgct
    20701 ggatcttggt ctggtggtac gcgaatatct gtcacagtat ccgcgtgcac gtcactttga
    20761 cgttgcgcgc attgttattg atcaggcggt acgtcttggc gtagcgcaag cagatttcac
    20821 cggactgcca gcgaaatggc agccgattaa tgattacgga gccaaggtac aggcgcatgt
    20881 catcgacaaa tattgaacaa gtgatgccgg ttaagctggc gcaggcgctg gcgaatccgt
    20941 tatttccggc gctggacagc gccttacgtt caggccgcca tattggcctc gacgaactgg
    21001 ataatcatgc attcctgatg gattttcagg aatatctgga agagttttac gcgcgttata
    21061 acgttgagct tattcgtgca ccggaagggt tcttctattt acgtccacgt tccaccacgc
    21121 tgatccctcg ttccgtcttg tcggaactgg atatgatggt cgggaaaatc ctctgttatc
    21181 tctatctcag cccggaacgg ctggcgaatg aggggatttt cacccagcag gaactgtacg
    21241 acgaactgct caccctggcc gatgaagcga aactgctgaa actggtgaac aaccgttcaa
    21301 ccggttcaga cattgaccgt cagaagttgc aggaaaaagt acgttcttcg ctcaaccgtc
    21361 tgcgtcgttt aggtatggtg tggtttatgg gccacgacag cagcaagttc cgcattaccg
    21421 aatcggtgtt ccgcttcgga gccgatgtgc gtgctggcga cgatccccgt gaagcacagc
    21481 gtcgcctgat tcgtgatggc gaagcaatgc cgattgaaaa tcatctgcaa ctcaacgatg
    21541 aaaccgaaga gaatcagcca gatagcggag aggaagaata atgattgaac gcggtaaatt
    21601 tcgctcactg acgctgatta actggaacgg cttctttgcc cgaacttttg accttgacga
    21661 gctggtcacg acgctttctg gcggtaacgg ggcgggtaaa tccaccacca tggcggcgtt
    21721 cgttacggcg ctgatccccg acctgaccct gctgcatttc cgtaacacta cggaagccgg
    21781 ggccaccagc ggttcgcgcg ataaaggtct gcacggtaag ctgaaagcgg gtgtctgtta
    21841 ttcgatgctc gacaccatta actcgcgcca ccagcgcgtg gtggtcggtg tgcgtctgca
    21901 acaggttgcc ggacgcgatc gtaaagtgga tatcaaacca tttgccattc agggactgcc
    21961 gatgtcggtg cagccgacac agctggtgac cgaaactctg aacgaacgcc aggcgcgcgt
    22021 gctgccgctt aacgagctga aagacaagct cgaggcgatg gaaggcgtgc agtttaaaca
    22081 gttcaactcc attaccgatt accactcgct gatgttcgat ctgggcatca ttgcgcgtcg
    22141 tctgcgctcc gcatctgacc gtagcaaatt ctatcgtctg atcgaagctt cgctgtatgg
    22201 cgggatctcc agtgccatta cccgttctct gcgcgactac ctgttgccgg aaaacagcgg
    22261 cgtgcgtaaa gcgttccagg acatggaagc ggcgctgcgt gaaaaccgca tgacgctgga
    22321 agcgattcgt gtcacccagt cggaccgtga cctgtttaag catctgatca gcgaagctac
    22381 caactacgtg gcggcggact acatgcgtca cgccaacgag cgccgtgtcc atctcgataa
    22441 ggccctggag tttcgtcgcg aactacatac ttcgcgtcag caactggcgg cggagcagta
    22501 caaacacgtc gatatggcgc gtgagctggc agagcacaac ggtgccgaag gcgatctgga
    22561 agcggattat caggcggcca gcgatcacct gaacctggtg caaaccgcac tgcgtcagca
    22621 ggagaagatc gaacgctacg aagcggatct cgatgagctg cagatccgtc tggaagagca
    22681 aaacgaagtg gtggcagaag ccatcgaacg ccaggaagag aacgaggctc gcgcggaagc
    22741 tgccgaactg gaagtggacg agctgaaaag ccagcttgct gactaccagc aggcgctgga
    22801 cgtccagcaa acgcgcgcga tccagtacaa ccaggcgatt gctgcgctta atcgtgccaa
    22861 agaactgtgc catctgccgg acttaaccgc cgacagcgcc gccgaatggc tggaaacctt
    22921 ccaggcgaaa gagctggaag cgactgaaaa aatgctctct cttgagcaga aaatgagcat
    22981 ggcgcaaacc gcgcacagcc agtttgagca ggcttatcag ctggtggtgg caatcaacgg
    23041 cccgctggcg cgtaacgagg cgtgggatgt cgctcgcgaa ctattgcgcg aaggggtcga
    23101 tcagcgtcac ctggcagagc aggttcagcc gttgcggatg cgcttaagcg aactggaaca
    23161 gcgtctgcgc gaacagcaag aagctgagcg tctgctggca gatttctgca aacgtcaggg
    23221 caagaatttt gacattgacg agctggaagc cctgcatcag gagctggaag cacgcattgc
    23281 ctctctttcc gatagcgtgt ctaacgcccg tgaagagcgg atggcactgc gccaggagca
    23341 ggaacagctg cagtctcgca ttcagagttt gatgcagcgt gcaccggttt ggctggcagc
    23401 gcaaaacagt ctcaaccagt tgagcgaaca gtgcggcgaa gagttcacct ccagccagga
    23461 cgtcactgaa tatctgcaac agttgctgga gcgtgagcgc gaggccattg ttgaacgcga
    23521 tgaagtaggc gcgcgcaaaa acgccgtcga tgaagagatc gaacgtttaa gccagcctgg
    23581 cggctctgaa gatcagcgtc tgaacgcgct agcggagcgt tttggtggtg tgctgctgtc
    23641 agaaatttat gacgacgtta gcctggaaga tgcgccgtac ttctcagcgc tgtatggccc
    23701 gtcacgccac gccatcgtgg tgccagatct gtcacaggta accgaacacc tggaaggttt
    23761 gaccgattgc ccggaagatc tctatttgat cgaaggggat ccgcagtcat tcgatgacag
    23821 cgtgttcagc gttgatgagc tggaaaaagc ggtagtggtg aaaatcgccg atcgtcagtg
    23881 gcgttattca cgtttcccgg aagtgccgct gtttggtcgt gctgcgcgtg aaagccgtat
    23941 tgaaagcctc catgccgagc gtgaagtgct ttccgaacgc ttcgccacgc tctcctttga
    24001 tgtacagaaa acccagcgtc tgcatcaggc gttcagccgc tttatcggca gtcatctggc
    24061 ggtggcgttt gagtctgacc cggaagcaga aatccgtcaa ctgaacagcc gtcgcgtcga
    24121 actggagcgg gcgttgagta atcatgaaaa tgataaccag cagcagcgta ttcagtttga
    24181 gcaggcgaaa gaaggcgtta cggcgctgaa ccgcattctg ccgcgtctca acctgttggc
    24241 tgatgacagc ctggcggatc gcgtcgatga aatccgcgaa cgtctggatg aagcccagga
    24301 agccgcgcgt tttgttcagc agtttggcaa tcaactggcg aaactggaac cgatcgtttc
    24361 ggtattgcag agcgacccgg aacagttcga acagttaaaa gaagattacg cgtactctca
    24421 gcagatgcag cgcgatgccc gtcagcaggc gtttgccctg acggaagtgg tgcagcgtcg
    24481 tgcgcacttt agctactctg actcggcaga aatgcttagc ggtaacagcg atctcaacga
    24541 aaaactgcgt gaacgtctgg aacaggcgga agcggagcgt acccgcgctc gcgaagcgtt
    24601 gcgcggtcac gcagcgcagt tgagtcagta caaccaggtg ctggcttcgc tgaaaagttc
    24661 ttacgacacc aaaaaagagc tactcaacga tctgcaacgt gaattgcagg atatcggcgt
    24721 gcgtgctgat agcggggcag aagagcgggc acgtattcgc cgtgacgagc tgcatgcaca
    24781 actgagcaat aaccgttcgc gccgcaatca actggaaaaa gcgctcacct tctgcgaagc
    24841 ggagatggat aacctgaccc gcaaactgcg caagctggag cgtgattact ttgagatgcg
    24901 cgagcaggta gtgaccgcga aagcgggctg gtgtgcagtc atgcgcatgg tgaaagataa
    24961 cggcgttgag cgccgcttac accgtcgtga gctggcttat ctctccgctg atgatttgcg
    25021 ttccatgtcg gataaggcgt taggtgcgct gcgtctggcg gtggcggata acgaacatct
    25081 gcgcgacgtg ctgcgcatgt cggaagatcc caaacgtccg gagcgtaaaa ttcagttctt
    25141 cgtggcggtt tatcagcatc tgcgtgaacg tattcgtcag gatattattc gtaccgatga
    25201 tccggtggaa gctatcgaac agatggagat cgaacttagc cgtctgaccg aagagttaac
    25261 ctcccgtgaa cagaaactgg cgatcagttc ccgcagcgtg gcgaacatca ttcgcaaaac
    25321 cattcagcgc gagcagaacc gtatccgtat gctcaaccag gggttgcaga acgtatcgtt
    25381 tggtcaggtg aacagcgtgc gcctcaacgt gaacgtgcgt gaaacgcacg ccatgctgct
    25441 ggatgtgctc tctgaacagc atgagcagca tcaggatctg tttaacagca accgtctgac
    25501 cttctcggaa gcgctggcga aattgtatca gcgtcttaat ccgcagattg atatggggca
    25561 gcgcacgccg cagaccattg gcgaagaact gctggattac cgcaactatc tggaaatgga
    25621 agttgaggtt aaccgtggtt ccgatggctg gttgcgcgca gagtctggtg cattgtcgac
    25681 cggtgaggcg attggtaccg gtatgtcgat tctggtgatg gtggtacaaa gctgggaaga
    25741 tgaatctcgc cgcctgcgcg gtaaagatat ctctccttgc cgcctgctgt tcctcgatga
    25801 agcagcgcga ctggatgctc gttctatcgc cacgctgttt gaattgtgtg agcgtttgca
    25861 aatgcagctc atcatcgcag cgccggaaaa tatcagcccg gagaaaggca ccacctataa
    25921 actggtgcgt aaagtcttcc agaataccga acatgttcat gtcgtcggcc tgcgaggatt
    25981 tgcgccgcaa ctccctgaaa cgcttccagg aagtgacgaa gcgccttctc aggcgagtta
    26041 aaattaaggc ggcagcaatg ccgccttttc tttttccgaa aactccgttt ctgcactaaa
    26101 aaagtggcac attacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac
    26161 gtatcggcgt ttatatactg aagataagcc tgatgagtaa caggcttgct cgtcatactt
    26221 tcgtgagtat tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat
    26281 gataaaaaca gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca
    26341 atcagtttgt gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct
    26401 gaagtaatcc ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag
    26461 gcgcaagcca cggcaataat ggcggggatc ctgccattgc ctgaaggtgc ggcagaaaaa
    26521 gcccgcacgc aaatcgaatc tcaattaccc gcaggttaca agccggttta tcttaaccag
    26581 cttcaactgt tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgctgtt
    26641 aaagccttcc agcaacagct ggcagaggtg gcgattgccg gtttccagcc gcagtttaat
    26701 aaatgggtag agttactgac cgatcctggt gttaacggga tggcacgcga cgtggtgctc
    26761 tctgatgcga tgatgggcta tctccatttc attgcaaata ttccggtcaa aggcactcgc
    26821 tggctatata gcagtaaacc ttatgcgctt gcaacgccgc cgctttcggt gattaaccaa
    26881 tggcagcagg cgctggataa aggtcaattg cctacgtttg ttgcaggact ggcaccgcag
    26941 catccgcaat atgcggtgat gcatgaatcg ttactggcct tactcagtga caccaaaccg
    27001 tggccccaac tgaccggcaa agcaacgttg cgcccagggc agtggagtaa cgacgtaccg
    27061 gcgttgcgtg aaatattgca acgcacaggc atgttggacg gggggccgaa aattactcta
    27121 cctggcgatg acacgccaac tgacgcggta gtcagcccat ccgctgttac tgttgaaaca
    27181 gcagaaacta agccgatgga taagcaaacg acgtctcgta gtaaacctgc gcctgccgtt
    27241 cgcgccgcct acgataatga actggtggaa gccgttaaac gttttcaggc atggcaagga
    27301 ttgggggcag atggtgctat tggcccggca acgcgtgact ggttaaacgt aacgcccgcc
    27361 cagcgtgctg gcgtgttggc tctcaacatc cagcgattgc gcttgctgcc aacagagctt
    27421 tctaccggga tcatggttaa cattccggcc tattcgctgg tctactatca gaacggcaat
    27481 caggtgctgg attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc
    27541 agtgccctta acaatgtagt ggtaaacccg ccgtggaacg tacctccaac tctggcacgc
    27601 aaagatattc tgccgaaagt gcgcaacgat ccgggatatc tcgaaagcca tggctatacg
    27661 gtgatgcgcg gctggaacag cagagaagcg attgacccat ggcaggttga ctggtctaca
    27721 atcacggcct cgaatttacc gtttcgcttc caacaggctc caggcccacg gaactcgctg
    27781 gggcgctata aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac
    27841 cacaatctgt tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa
    27901 gcttccgatc tggcgaatat gctgttgcag gatgcaggct ggaatgacaa acgtatttct
    27961 gatgcgctga agcagggtga tacacgttac gtcaatattc ggcagtcgat tccggtgaat
    28021 ctctactacc tgacggcctt tgttggtgca gatggtcgta cccagtatcg tacagatatt
    28081 tacaattatg atctgcctgc gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta
    28141 atcaggtaaa tgaagtagtt cggggaaatc agttgtcgta ataattagca tgattggggg
    28201 caattctctg cagcccccgt cactgctggg gttgagtcat cttgacgtct gctttacggg
    28261 cggttaaggt gcctcttgtg cgccagaagt gcatataaac gataacattg acctgtagac
    28321 ttgattatca tggacaaatt cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt
    28381 gcactcggtg ccgccatcct gccgacccct gcgtttgcaa cactctctac cccacgcccg
    28441 cgcattttga cactcaataa tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat
    28501 ggcagaggct atattcagga agaattggca aaacttaacc attttttccg cgattaccgc
    28561 gcgaacaaaa taaagtccat cgacccagga ttattcgacc agttgtatcg cctgcaaggg
    28621 ttattaggca cccgcaaacc ggtgcaactc atttccggtt atcgttccat tgataccaac
    28681 aatgaactac gcgcccgcag ccgtggagta gcgaagaaaa gctatcacac taaaggccag
    28741 gcgatggatt tccatattga aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct
    28801 atgcgcgcag gtggtgtagg atattaccca cgtagtaact ttgtgcatat tgataccggg
    28861 ccagcacggc actggtagta attgcttaac gaaacagggg cagtatgaac tatcgtatta
    28921 ttccggtcac cgcattctcc cagaactgtt cattgatctg gtgtgaacaa acccgcctgg
    28981 ccgcactggt cgatcctggc ggcgatgcgg aaaaaatcaa acaggaagtt gacgccagcg
    29041 gcctgacact gatgcagatc ctgctgacgc atggtcatct ggaccacgtt ggcgcagcgg
    29101 cggaactggc gcaacattac ggcgtgccgg tttttggccc ggaaaaagaa gatgagttct
    29161 ggctgcaagg cttgcctgcg caaagtcgta tgtttggtct ggaagagtgc cagccgctga
    29221 cgccagatcg ttggctgaac gaaggcgata ccatcagcat agggaatgtg actttacagg
    29281 tgttacattg ccctgggcat acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc
    29341 tgctgatttc tggcgatgtt attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg
    29401 gcgatcataa tcaactgatt tcttcaatca aagataaatt gctgccactg ggtgatgacg
    29461 tgacatttat tccgggtcac ggaccattat ccacacttgg ttatgaacgc ctgcataatc
    29521 ccttcctgca agacgaaatg cccgtctggt aaggcacaca taaaaaagcc cgcttttcag
    29581 cgggcttcat tgtttttaac gcttacagca ctgccacaat cgcttcgcac agcggagcca
    29641 tgttatctgg tgtcatcccg gccacgttta cgcgaccaga agcaacagca tacacgccaa
    29701 actcttcgcg cagacgcagc acttgttctt ttgtcaggcc actgaaggag aacatgccgt
    29761 tctgtttgat gataaagctg aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga
    29821 acaactgacg catacgctga atacgctggc gcatatcagt cagctcttgt tcccaaatcg
    29881 cacgtaacgc atcgttgctc aggatggtgg caacaacaga agcgccgtgt gctggtgggt
    29941 tagagtagtt agcgcgaatc gccgctttca tttggctgaa tgcgcgatca acggtttcac
    30001 tgtcggcagc aaccagagta caagcgccaa cacgctcgtt gtacaggcca aagtttttag
    30061 agtaggaact ggcaacaatc agctctttat gcatagccgc gaaagcgcgc agtccttcag
    30121 catcttcttc cagaccacgg gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc
    30181 ctttctcaac ggagagttgt gccagtgtct gccattgttc cagcgtaggg tcgataccgg
    30241 ttgggttgtg gcagcagcca tggaacagca ctacgtcgcc agcctgagct tcgttcaggc
    30301 tgttaatcag tgcatcgaag tcaagggtgt ggttttccgc atcataataa gcgtattcac
    30361 gaacttccag atctgcagag ttaaagacgc tcttatggtt cggccagctt gggttgctca
    30421 cccacactcg cttaacgctg gtattttttg ccaggaaatc ggcagctatg cgtagtgcgc
    30481 cagtgccacc cggagtctgt gccgtgcgag cacgtttgtc attgatcagg gcgctacctt
    30541 taccaaacag cagttcctga gtgcagcgac caaattcagg gatgccgtca atgccgaggt
    30601 aatttttggt ggtttcattt tcgagcagat actgttcagc ctttttcacg ctggtcagta
    30661 ccggggtttt gcccgtctca tctttataga caccaatccc gaggttaatt ttgccgggac
    30721 gttcatcggc acgaaacaga tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa
    30781 tgttctcaaa catgacgagg ttccattatg gttacagaag ggaagtccgc tatcagggta
    30841 acgggagatt tacaaaattc caactattac tgatgaaaac gcaggctgtt tttgcaagac
    30901 gtgagattgc tctggaaggc ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt
    30961 aacaaagagg tgtgctatta gaactggtaa acgataccca cagcaacggt gtcgtctgaa
    31021 cctacgccca gtttgttgtc agaatcgatc tggttgatga tgtagtcaac ataggtggac
    31081 atgtttttgt tgaagtagta ggttgcgccc acttcaaagt agttcaccag atcaacatca
    31141 ccgatacctt ctacgtcttt cgctttagat ttggtgtaag cgatggacgg acgcagaccg
    31201 aaatcgaact ggtattgcgc aactaacaga acgtcttgcg ttttgttggc gaagccgctg
    31261 atgtttgtaa atttattagt gatcggcgta gcgttacgcg tttcaccgta gttcgctgcc
    31321 aggtagatgt tgttcgcatc atacttcaga ccagtagccc actgttcagc ttttttaccg
    31381 ttgccaagaa gttgagcttc ttgcaggttg gtacggtcag ctgcaccata agcaccaacg
    31441 ataccaaagc cttcgtattc gtagctgata gaaccgccaa caccgtcgcc gttagagcgg
    31501 cgtgcagtgt cacgctcgtt tttacccagg tactgaacag cgaagttcag gccatcaacc
    31561 agaccaaaga agttggagtt acgataggta gcaacgccgc caacacgacc aacgaagaag
    31621 tcatcgctgt atgcagtatc accaccaaat tctggcagca tatcggtgta acccagtgca
    31681 tcataaacca caccgtagtt acggccgtaa tcgaaagaac caacgtcagc gtatttaaga
    31741 cccgcgaatg ccagacgcgt tttgttacca gtttgagcgt cagcgccttc agagttgtta
    31801 ccctggaagt tatattccca ctgaccataa ccggtcagat cggaattgat ttgagtttcc
    31861 cctttaaaac caagacgggc ataggtcatg tcgccattgc caccgtaact gttttcaccg
    31921 ttacccttgg agaaataatg cagaccgaca gctttaccgt acagatctac tttgttgcca
    31981 tctttgttat agatttctgc agcgtttgca gtacctgcta ctaacagagc agggacgatc
    32041 actgccagaa tattgcgctt catcattatt tattaccctc atggtttttt ttatgacacc
    32101 tgccactgcc gtcaataatt tctgtcaata aaattttacg gaactattga tgagagtttg
    32161 gtgtctttat gtgtctgcag gcatctttcc attcaaacta acgtttcgct accgtgaaag
    32221 tgctacaaag ataaagattt agtttcaaaa agaaaaaata tgtaactaaa agtaaa
//
LOCUS       DAZKIF010000047        32096 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZKIF010000047 DAZKIF010000000
VERSION     DAZKIF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.047"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            31..330
                     /locus_tag="ACHOTW_002135"
     CDS             31..330
                     /locus_tag="ACHOTW_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844244.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGGFPVTNIVLFLILIFFKKR"
     gene            complement(377..1264)
                     /gene="rarD"
                     /locus_tag="ACHOTW_002136"
     CDS             complement(377..1264)
                     /gene="rarD"
                     /locus_tag="ACHOTW_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP9844245.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1316..1783)
                     /gene="yigI"
                     /locus_tag="ACHOTW_002137"
     CDS             complement(1316..1783)
                     /gene="yigI"
                     /locus_tag="ACHOTW_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP9844246.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1948..2817
                     /gene="pldA"
                     /locus_tag="ACHOTW_002138"
     CDS             1948..2817
                     /gene="pldA"
                     /locus_tag="ACHOTW_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP9844247.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2950..4779
                     /gene="recQ"
                     /locus_tag="ACHOTW_002139"
     CDS             2950..4779
                     /gene="recQ"
                     /locus_tag="ACHOTW_002139"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9844248.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4843..5463
                     /gene="rhtC"
                     /locus_tag="ACHOTW_002140"
     CDS             4843..5463
                     /gene="rhtC"
                     /locus_tag="ACHOTW_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP9844249.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5525..6145)
                     /gene="rhtB"
                     /locus_tag="ACHOTW_002141"
     CDS             complement(5525..6145)
                     /gene="rhtB"
                     /locus_tag="ACHOTW_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP9844250.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6256..7278
                     /gene="pldB"
                     /locus_tag="ACHOTW_002142"
     CDS             6256..7278
                     /gene="pldB"
                     /locus_tag="ACHOTW_002142"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP9844251.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7286..8086
                     /gene="yigL"
                     /locus_tag="ACHOTW_002143"
     CDS             7286..8086
                     /gene="yigL"
                     /locus_tag="ACHOTW_002143"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP9844252.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKEPLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8162..9061
                     /gene="bioP"
                     /locus_tag="ACHOTW_002144"
     CDS             8162..9061
                     /gene="bioP"
                     /locus_tag="ACHOTW_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP9844253.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8949..9902)
                     /gene="metR"
                     /locus_tag="ACHOTW_002145"
     CDS             complement(8949..9902)
                     /gene="metR"
                     /locus_tag="ACHOTW_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP9844254.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10138..12399
                     /gene="metE"
                     /locus_tag="ACHOTW_002146"
     CDS             10138..12399
                     /gene="metE"
                     /locus_tag="ACHOTW_002146"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9844255.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLCDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12439..13254)
                     /gene="ysgA"
                     /locus_tag="ACHOTW_002147"
     CDS             complement(12439..13254)
                     /gene="ysgA"
                     /locus_tag="ACHOTW_002147"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP9844256.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13516..14277
                     /gene="udp"
                     /locus_tag="ACHOTW_002148"
     CDS             13516..14277
                     /gene="udp"
                     /locus_tag="ACHOTW_002148"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP9844257.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14418..15845
                     /gene="rmuC"
                     /locus_tag="ACHOTW_002149"
     CDS             14418..15845
                     /gene="rmuC"
                     /locus_tag="ACHOTW_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP9844258.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15940..16695
                     /gene="ubiE"
                     /locus_tag="ACHOTW_002150"
     CDS             15940..16695
                     /gene="ubiE"
                     /locus_tag="ACHOTW_002150"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP9844259.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16709..17314
                     /gene="ubiJ"
                     /locus_tag="ACHOTW_002151"
     CDS             16709..17314
                     /gene="ubiJ"
                     /locus_tag="ACHOTW_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP9844260.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17311..18951
                     /gene="ubiB"
                     /locus_tag="ACHOTW_002152"
     CDS             17311..18951
                     /gene="ubiB"
                     /locus_tag="ACHOTW_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP9844261.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19030..19299
                     /gene="tatA"
                     /locus_tag="ACHOTW_002153"
     CDS             19030..19299
                     /gene="tatA"
                     /locus_tag="ACHOTW_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP9844262.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19303..19818
                     /gene="tatB"
                     /locus_tag="ACHOTW_002154"
     CDS             19303..19818
                     /gene="tatB"
                     /locus_tag="ACHOTW_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP9844263.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19821..20597
                     /gene="tatC"
                     /locus_tag="ACHOTW_002155"
     CDS             19821..20597
                     /gene="tatC"
                     /locus_tag="ACHOTW_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP9844264.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20639..21421
                     /gene="tatD"
                     /locus_tag="ACHOTW_002156"
     CDS             20639..21421
                     /gene="tatD"
                     /locus_tag="ACHOTW_002156"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP9844265.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21418..21906)
                     /gene="rfaH"
                     /locus_tag="ACHOTW_002157"
     CDS             complement(21418..21906)
                     /gene="rfaH"
                     /locus_tag="ACHOTW_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP9844266.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22073..23566
                     /gene="ubiD"
                     /locus_tag="ACHOTW_002158"
     CDS             22073..23566
                     /gene="ubiD"
                     /locus_tag="ACHOTW_002158"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP9844267.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23612..24313
                     /gene="fre"
                     /locus_tag="ACHOTW_002159"
     CDS             23612..24313
                     /gene="fre"
                     /locus_tag="ACHOTW_002159"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP9844268.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24505..25668)
                     /gene="fadA"
                     /locus_tag="ACHOTW_002160"
     CDS             complement(24505..25668)
                     /gene="fadA"
                     /locus_tag="ACHOTW_002160"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP9844269.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25678..27867)
                     /gene="fadB"
                     /locus_tag="ACHOTW_002161"
     CDS             complement(25678..27867)
                     /gene="fadB"
                     /locus_tag="ACHOTW_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP9844270.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28057..29388
                     /gene="pepQ"
                     /locus_tag="ACHOTW_002162"
     CDS             28057..29388
                     /gene="pepQ"
                     /locus_tag="ACHOTW_002162"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP9844271.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29388..30002
                     /gene="yigZ"
                     /locus_tag="ACHOTW_002163"
     CDS             29388..30002
                     /gene="yigZ"
                     /locus_tag="ACHOTW_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP9844272.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30041..31492
                     /gene="trkH"
                     /locus_tag="ACHOTW_002164"
     CDS             30041..31492
                     /gene="trkH"
                     /locus_tag="ACHOTW_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP9844273.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31504..32049
                     /gene="hemG"
                     /locus_tag="ACHOTW_002165"
     CDS             31504..32049
                     /gene="hemG"
                     /locus_tag="ACHOTW_002165"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP9844274.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7462 a         8115 c         8768 g         7751 t
ORIGIN      
        1 taaacaatat cttgggtgaa tggattctat atgggaaatg gtaacttttg gcagttgttc
       61 ttgggtaaag aaccttacag caaaaaatct gattttgtta aggcatggtt attgtggtgg
      121 gctattacca tccttgttct atttatttta agcctgacta cgacattaaa tttaagaagt
      181 ataggtatag ggttaagttt tgggttttgt caaatccttg gtagatatac gatgtctcta
      241 ggatggagta agtactgggg agttttgggg ggatttcctg taacaaatat tgttcttttt
      301 cttatcttaa tttttttcaa aaaaagataa ataagtcagt gggtaggctc tttaaacggc
      361 ctacccatgt taattcttaa ctttttctac gctgcgtata aatcgcatcc atcacaaaaa
      421 ttgccagcgc cacccaaata aaggtgaaag tcaccatctt atcggcaccc ggtttttcac
      481 cataaaacgt cacagccagc aggaacatca gcgtcgggcc aatgtactgg aaaaagccta
      541 acgttgagag acgaaggcgc gtggcagcgg cggtaaaaca caacagcggt acggtagtga
      601 caataccggc ggcgatcagc agtaaattca gcgacatcgg gttttgcccc atatggctgg
      661 ttgagctgtc ggcaatagca aacaggtaaa tcgccgccac gggcagcagc cacatggttt
      721 caattaacat gccggtttgt gcttctaccg caatcttctt gcgcaccagg ccgtagaagg
      781 caaaactaaa tgccagcccc agcgcgataa taggtagcga accaaaagtc cacagctgga
      841 ctaacacgcc acatatcgcc agaatcaccg ccagccattg catccggcgg aatcgctcgc
      901 cgaggaaaat catccccagc acaatgttca ccagcgggtt aataaagtaa ccaaggctcg
      961 cttccagcat atggtgattg ttcaccgccc agataaacag tagccagttg ccaccaatca
     1021 gcacggcaga gactgccagc ataaaaattt tctgtggcgt ctgaatcagc gtttttaaat
     1081 aggaccactg gcggcaaatg ctcatcagca ccaccataaa gaaaaacgac cagatcacgc
     1141 gatgcgtcag gatctcatcg gcgggcacgt agtaaatcaa cttgaagtac gctggggcta
     1201 taccccaaat aaaataagcg gcaagagcga gtaatacgcc ctgccgcgtt tgttttgcat
     1261 ccatcgggaa tactcatttt taattggtaa cagcagttta cctgctttta tgtcttcaac
     1321 ctaccatata ggtggcggtg gcactggcaa tataaagctg ttcttcattg tgtaattcaa
     1381 cgcgggcgac ggcgacttta ttgcctgcac gcaacaggct actggtagca gtaaaacgct
     1441 cgcctctgcc tgggcgcaga taatcgacgc gaagatcaat ggtccccatc cgcgatagcc
     1501 gctggcgtag ttcatcttca ctgatggttt cgtggcgggt taaggtactt cctacgcaca
     1561 ccagaccggc ggcgacatcc agcgccgacg caatgacccc gccgtgcaaa atgctttgcg
     1621 cccagttgcc caccatcatt ggctgatttt taaaggccag ctgtgcgaac tctttttcgt
     1681 aacgctccag ttccatcccc aatgcgcggt taaatggcat gtgataaaca aacatctcac
     1741 ccactaattt cagggcttgt tcagcggtca gtacggcaga catatcatcc ttacacttca
     1801 ttggttaatg aaatgttgat tttatgattc tttgttgttg gtttctactt taggaaggga
     1861 taactaacgg caatggagtt aagtatgtaa aataccccgc agaaaaacat tcaccttatc
     1921 aataattcgt tacggagaac acgaccgatg cggactctgc agggctggtt gttgccggtg
     1981 tttatgttgc ctatggcagt atatgcacaa gaggcaacgg tgaaagaggt gcatgacgcg
     2041 ccagcggtgc gtggcagtat tatcgccaat atgctgcagg agcatgacaa tccgttcacg
     2101 ctctatcctt atgacaccaa ctacctcatc tacacccaaa ccagcgatct gaacaaagaa
     2161 gcgattgcca gttacgactg ggcggaaaat gcgcgtaagg atgaagtaaa gtttcagttg
     2221 agcctggcat ttccgctgtg gcgtgggatt ttaggcccga actcggtgtt gggtgcgtct
     2281 tatacgcaaa aatcctggtg gcaactgtcc aatagcgaag agtcttcacc gtttcgtgaa
     2341 accaactacg aaccgcaatt gttcctcggt tttgccaccg attaccgttt tgcaggttgg
     2401 acgctgcgcg atgtggagat ggggtataac cacgactcta acgggcgttc agacccgacc
     2461 tcccgcagct ggaaccgcct ttatactcgc ctgatggcag aaaacggtaa ctggctggta
     2521 gaagtgaagc cgtggtatgt ggtgggtaat actgacgata acccggatat caccaaatat
     2581 atgggttact accagcttaa aatcggctat cacctcggtg atgcggtgct cagtgcgaaa
     2641 ggacagtaca actggaacac cggctacggc ggcgcggagt taggcttaag ttacccgatc
     2701 accaaacatg tgcgccttta tactcaggtt tacagcggct atggcgaatc gctcatcgac
     2761 tataacttca accagacccg tgtcggtgtg ggggttatgc taaacgattt gttttgatga
     2821 acggttgagt ggttggcaaa tctggaatcc agcatccagg attaccctct cagagactaa
     2881 aagcattgca gtttctcgcg caggcgctga aaatagcgcc tgtttttatt tcaggcaatc
     2941 ggggtgaatg tggcgcaggc ggaagtgttg aatctggagt ccggagctaa acaggtttta
     3001 caagaaacct ttggctacca acagtttcgc cccggccagg aagaaattat cgacactgtg
     3061 ctttccggtc gcgattgcct ggtcgtcatg cccaccggtg gcggaaaatc cctttgctat
     3121 caaattcctg ccttattgct aaacggcctt accgtggttg tttcaccgct gatttcgttg
     3181 atgaaagatc aggtggatca actgcaagcc aacggcgtgg cggcggcgtg ccttaactcg
     3241 acgcaaaccc gcgaacagca acttgaagtg atgacaggct gccgcaccgg gcaaattcgt
     3301 ctgctttata tcgccccgga acgcctgatg ctggataact ttcttgagca tctggcgcac
     3361 tggaatccgg tgttattagc cgttgatgaa gcgcactgta tctcccaatg gggccacgat
     3421 ttccgcccgg aatatgccgc gctcggtcag ttgcgccagc ggttcccgac gctgccgttt
     3481 atggcgctga ccgccacagc cgacgacacc acgcgccagg atatcgtgcg cctgctgggg
     3541 ctgaacgatc cgctgattca aatcagcagt tttgaccgtc cgaatattcg ctacatgctg
     3601 atggagaaat tcaaaccgct cgatcagttg atgcgctacg tgcaggaaca gcgcggtaag
     3661 tccggcatta tctactgcaa cagccgggcg aaagtagaag acaccgctgc gcgcctgcaa
     3721 agcaagggta ttagcgcggc ggcctatcat gccgggctgg aaaataatgt ccgcgccgac
     3781 gtgcaggaga aattccagcg cgatgacctg caaattgtgg tggcgacggt ggcgttcggc
     3841 atgggcatca ataaaccaaa cgttcgcttc gtggtccact ttgatattcc gcgcaatatc
     3901 gaatcctatt atcaggaaac cggtcgcgcc gggcgtgatg gcctgcctgc ggaagcgatg
     3961 ttgttttacg atccagctga tatggcgtgg ctgcgccgtt gtctggaaga gaagccgcag
     4021 gggcagttgc aggatatcga gcgccacaaa ctcaatgcga tgggcgcgtt tgccgaagcg
     4081 caaacttgcc gtcgtctggt attgctgaac tattttggcg aagggcgtca ggagccgtgc
     4141 gggaactgcg atatctgcct cgatccgccg aaacagtacg acggttcaac cgatgctcag
     4201 attgcccttt ccaccattgg tcgtgtgaat cagcggtttg ggatgggtta tgtggtggaa
     4261 gtgattcgtg gtgctaataa ccagcgtatc cgcgactatg gtcatgacaa actgaaagtc
     4321 tatggcatgg gccgtgataa aagccatgaa cattgggtga gcgtgatccg ccagctgatt
     4381 cacctcggcc tggtgacgca aaatattgcc cagcattctg ccctacaact gacagaggcc
     4441 gcgcgcccgg tgctgcgcgg cgaatcctct ttacaacttg ccgtgccgcg tatcgtggcg
     4501 ctcaaaccga aagcgatgca gaaatcgttc ggcggcaact atgatcgcaa actgttcgcc
     4561 aaattacgca aactgcgtaa atcgattgcc gatgaaagta atgtcccgcc gtacgtggtg
     4621 tttaacgacg caaccttgat tgagatggct gaacagatgc cgatcaccgc cagcgaaatg
     4681 ctcagcgtta acggcgttgg gatgcgcaag ctggaacgct ttggtaaacc gtttatggcg
     4741 ctgattcgtg cgcatgtcga tggcgacgac gaagagtagt cagcagcata aaaaagtgcc
     4801 aggatgaaga ctccgtaaac gtttcccccg cgagtcaaat gtatgttgat gttatttctc
     4861 accgtcgcca tggtgcacat tgtggcgctt atgagtcccg gtcccgattt cttttttgtc
     4921 tctcagaccg ctgtcagtcg ttcccgtaaa gaagcgatga tgggcgtgct gggtattacc
     4981 tgcggcgtta tggtttgggc agggattgcg ttgcttggcc tgcatttgat tatcgaaaaa
     5041 atggcctggc tgcatacgct gattatggtt ggcggtggcc tgtatctctg ctggatgggt
     5101 taccagatgc tgcgcggcgc gctgaaaaaa gaggcggttt ctgcacctgc gccacaggtc
     5161 gagctggcga aaagtgggcg cagtttcctg aaaggtttac tgaccaatct cgctaatccg
     5221 aaagcgatta tctactttgg ctcggtgttc tcattgtttg tcggtgataa cgttggcact
     5281 accgcgcgct ggggcatttt cgcgttgatt attgtcgaaa cgctggcgtg gtttactgtg
     5341 gtggccagcc tgtttgccct gccgcaaatg cgccgtggtt atcaacgtct ggcgaagtgg
     5401 attgatggtt ttgccggggc gttatttgcc ggatttggca ttcatttgat tatttcgcgg
     5461 taatgccaga cgcgtcttca gagtaagtcg gataaggcgt ttacgccgca tccgacatta
     5521 tttttcacgc atgcctcgcc gatgctaaca gcgctcccac cagcataaac aacgagccga
     5581 aaatcttatt cagcgccttc atctgctttg gtcctttaat ccatagagca atccgttgag
     5641 caagggtggc gtaaccgatc atcacaataa tatcgaccac aatagtggtg acaccgagca
     5701 cgatatactg catcagttgc ggctgttgcg gcatgatgaa ttgcggaaat agcgccgcca
     5761 gaaacacaat acttttggga ttggtgagat tcacaaaaac tgcgcgctgg aacaaatgtc
     5821 gacgcgattg agtagaggcc agcgatttaa gatcaattgc gccagcggcg cgccattgct
     5881 gtattcccag ccaaatcaag taagccgcgc ctgcccactt caacacttca aacgcaatca
     5941 ctgagcggga aaatagcgtc cccaacccca cgccaaccag cacaatatga atcgccagtc
     6001 cggtctgaag cccggcaata gacgccaccg cgccgcgata accgtggttg agcgaggtgg
     6061 tcatagtgtt gattgcacca gagcctggcg acaggcttaa aatgattgat gtcagtaggt
     6121 aggcaaacca ccattctaag gtcatgatga actcccggtg tgtctatttt tgtgccacaa
     6181 tacgctactg tcgcagcgtt gtgtcaggca cgctaaaaaa aacgatttta cgtggtttaa
     6241 gaggcagatt acccgatgtt tcagcagcaa aaagactggg aaacaagaga aaacgcgttt
     6301 gctgctttta ccatgggacc gctgactgat ttctggcgtc agcgtgatga agcagagttt
     6361 actggtgtgg atgacattcc ggtgcgcttt gtccgttttc gcgcacagca ccatgaccgg
     6421 gtggtagtca tctgcccggg gcgtattgag agttacgtaa aatatgcgga actggcctac
     6481 gatttgttcc atttggggtt tgatgtctta atcatcgacc atcgcgggca gggacgttcc
     6541 ggtcgcctgt tagccgatcc gcatctcggg catgttaatc gctttaatga ttatgttgat
     6601 gatctggcgg cattctggca acaggaggtc cagcccggtc cgtggcgtaa acgctatata
     6661 ctggcacatt cgatgggcgg tgcgatctcc acattatttc tgcaacgcca tccaggtgta
     6721 tgtgacgcca ttgcgctaac tgcgccaatg tttgggatcg tgattcgtat gccgtcattt
     6781 atggcacggc agatcctcaa ctgggccgaa gcgcatccac gtttccgtga tggctatgca
     6841 ataggcaccg ggcgctggcg cgcgttgccg tttgctatca acgtactgac ccacagcaga
     6901 cagcgatatc gacgtaactt acgcttctat gctgatgacc caacgattcg cgtcggtggg
     6961 ccgacctacc attgggtacg cgaaagtatt ctggctggcg aacaggtgtt agccggtgcg
     7021 ggtgatgacg ccacgccaac gcttctcttg caggctgaag aggaacgcgt ggtggataac
     7081 cgcattcatg accgtttttg tgaactccgc accgccgcgg gccatcctgt cgaaggggga
     7141 cgaccgctgg taattaaagg tgcgtaccat gagatccttt ttgaaaagga cgcaatgcgc
     7201 tcagttgcgc tccacgccat cgttgatttt ttcaacaggc ataactcacc cagcggaaac
     7261 cgctctacag aggtttaaat ttcttatgta ccaggttgtt gcgtctgatt tagatggcac
     7321 gttactttct cccgaccata cattatcccc ttacgccaaa gaacccctga agctgctcac
     7381 cgcgcgcggc atcaactttg tgtttgcgac cggtcgtcac cacgttgatg tggggcaaat
     7441 tcgcgataat ctggagatta agtcttacat gattacctcc aatggtgcgc gcgttcacga
     7501 tctggatggt aatctgattt ttgctcataa cctggatcgc gacattgcca gcgatctgtt
     7561 tggcgtagtc aacgacaatc cggacatcat tactaacgtt tatcgcgacg acgaatggtt
     7621 tatgaatcgc catcgcccgg aagagatgcg cttttttaaa gaagcggtgt tcaaatatgc
     7681 gctgtatgag cctggattac tggagccgga aggcgtcagc aaagtgttct tcacctgcga
     7741 ttcccatgaa caactgctgc cgctggagca ggcgattaac gctcgttggg gcgatcgcgt
     7801 caacgtcagt ttctctacct taacctgtct ggaagtgatg gcgggcggcg tttcaaaagg
     7861 ccatgcgctg gaagcggtgg cgaagaaact gggctacagc ctgaaggatt gtattgcgtt
     7921 tggtgacggg atgaacgatg ccgaaatgct gtcgatggcg gggaaaggct gcattatggg
     7981 cagtgcgcac cagcgtctga aagaccttca tcccgagctg gaagtgattg gtactaatgc
     8041 cgacgacgcg gtgccgcatt atctgcgtaa actctattta tcgtaatcgt tctttatttg
     8101 gtcagttgtc aacctgatac ttcgctacaa tggatactcg ttaatcaaag agttttccat
     8161 tgtggcgcta cttatcatca ccacgattct gtgggccttc tcctttagct tttatggcga
     8221 gtaccttgcg gggcacgtcg atagctattt tgcggtgctg gtgcgcgttg gcctggcggc
     8281 actcgttttt ctgccgtttc tgcgtacccg tggcaatagc ctgaaaacag tcggcctgta
     8341 tatgctggtg ggcgcgatgc agctcggcgt gatgtatatg ctgagtttcc gcgcttatct
     8401 ctacctgacg gtttccgaac tgctgctgtt caccgtactg acgccgctct acatcacgct
     8461 gatttatgac atcatgagta agcgccgtct gcgctgggga tatgctttta gtgccttgtt
     8521 ggcggtgatt ggtgccggga ttattcgcta tgatcaggtc accgaccatt tctggactgg
     8581 cttgttgctg gtgcaactct ccaatatcac ttttgccatc ggcatggtgg gttacaaacg
     8641 cctgatggaa actcgcccga tgccgcagca taacgccttt gcgtggttct atcttggcgc
     8701 gttcctggtg gcggtaattg catggttctt gctgggaaat gcgcagaaaa tgccgcaaac
     8761 cacgttgcaa tggggcattc tggtgtttct tggcgtggtg gcttccggga ttggctactt
     8821 tatgtggaac tacggcgcaa cccaagtaga cgccggaacg ctgggcatta tgaataatat
     8881 gcacgttccg gcagggctgc tggttaacct ggctatctgg caccaacagc cgcactggcc
     8941 aacgtttatt acaggcgcgc tggtgatcct ggcctcactg tgggtgcatc gtaagtgggt
     9001 cgctccgcgc tcttcacaaa cggcagatga tcgcaggcgt gattgcgcgc tgagcgaata
     9061 aacgcttccg taactggctg acgctgctcg ccatcgcgca cggcggcgta cagtcggctc
     9121 cacaagcctt cgcccagggt tttggtcacc accagaccct ggcgctcaaa actctctact
     9181 acccaatgtg gtagcgcggc aatacccatc cgcgcggcaa ccatctgaat caacaataag
     9241 gtgttatcga cgcttttcag cgacgggcta acgcctgcgg gctggagaaa atgccgccag
     9301 acatccagtc ggctacgctg caccggataa atcaacagcg tttcgctggc gagatcttcc
     9361 ggcgtgattc gcgttttcgc cgccagtgga tggtcaggtg ctaacaccag acgcacttca
     9421 tagtcgaaca tcggcgaata atgcaggcca ctacgcggca gaatatcgga ggtcatcacc
     9481 agatccagct cgccctgctg caacgcaggc tgcgggtcaa acgttacgcc cgatttaaaa
     9541 tccatctcca cctgcggcca gttcttatgg aaattttcta atgcgggcgt cagccactga
     9601 atacagctat ggcactcaat ggcaatgcgc aggcgcgtct gttgcggttc attgcaggct
     9661 tgcagcgcct ggctaatttg cggcagcacc tggtttgcca gttgcaacag gatttccccc
     9721 tgtggagtaa agcgtagcgg ctggctctta cgcacaaata gccggaagcc aaggcgttgt
     9781 tccagatcgc taaactggtg agacagggcg gattgcgttt gatgcaacgt cgccgcagcg
     9841 gctgcgagag agccgcagtt ccgcaacgct tgtagcgttt tcaggtgttt tacttcgatc
     9901 atgaaagtcc ttcacttcgg catgaataat ttgcgcttga ggaatataca gtaaccgcca
     9961 attatggatg tgtaaacatc tggacggcta aaatccttcg tcttttaaat ttatggtgcg
    10021 ttggctgcgt ttctccaccc cggtcactta cttcagtaag ctcccgggga tgaataaact
    10081 tgccgccttc cctaaattca aaatccatag gatttacata taattagagg aagaaaaatg
    10141 acaatattga atcacaccct cggtttccct cgcgttggcc tgcgtcgcga gctgaaaaaa
    10201 gcgcaagaga gttattgggc ggggaactcc acgcgtgaag aactgctggc ggtagggcgt
    10261 gaattgcgtg ctcgtcactg ggatcaacaa aagcaagcgg gtatcgacct gctgccggtg
    10321 ggcgattttg cctggtacga tcatgtactg accaccagtc tgctgctggg taacgttccg
    10381 gcgcgtcatc agaacaaaga tggttcggta gatatcgaca ccctgttccg tattggtcgt
    10441 ggacgtgcgc cgactggcga acctgcggcg gcagcggaaa tgaccaaatg gtttaacacc
    10501 aactatcact acatggtgcc ggagttcgtt aaaggccaac agttcaaact gacctggacg
    10561 cagctgctgg acgaagtgga cgaggcgctg gcgctgggcc acaaggtgaa acctgtgctg
    10621 ctggggccga ttacctggct gtggctgggg aaagtgaaag gtgaacagtt tgaccgcctg
    10681 agcctgctga acgacattct gccggtttat cagcaagtgc tggcagaact ggcgaaacgt
    10741 ggcatcgagt gggtacagat tgatgaaccc gcgctggtac tggaactacc acaggcgtgg
    10801 ctggacgcat acaaacccgc ttacgacgcg ctccagggac aggtgaaact gctgctgacc
    10861 acctattttg aaggcgtaac gccaaatctc gacacgatta ctgcgctgcc tgttcagggg
    10921 ctgcatgtgg accttgtaca tggtaaagat gacgttgttg aactgcacaa gcgcctgcct
    10981 tctgactggc tgctgtctgc gggtctgatc aatggtcgta acgtctggcg cgccgatctt
    11041 actgagaaat atgtgcaaat taaggacatt gtcggcaaac gtgatttgtg ggtggcatct
    11101 tcctgctcgt tgctgcacag ccccatcgac ctgagcgtgg aaacgcgtct tgatgcagaa
    11161 gtgaaaagct ggtttgcctt cgccctacaa aaatgccatg aactggcact gctgtgcgat
    11221 gcgctgaaca gtggtgacac ggcagctctg gcagagtgga gcgccccaat tcaggcgcgt
    11281 cgtcactcta ctcgtgtaca taatccggcg gtagaaaagc gtctggcggc gatcaccgcc
    11341 caggacagcc agcgtgcgaa tgtctatgaa gtgcgtgctg aagcccagcg tgcgcgtttt
    11401 aaactgccag cgtggccgac caccacgatt ggttccttcc cgcaaaccac ggaaattcgt
    11461 accctgcgtc tggatttcaa aaagggcaat ctcgacgcca ataactaccg cacgggcatt
    11521 gcggaacata tcaggcaggc cattgttgaa caggaacgtt tgggactgga tgtgctggta
    11581 catggcgagg ccgagcgtaa tgacatggtg gaatactttg gcgagcacct cgacgggttt
    11641 gtctttacgc aaaacggttg ggtacagagc tacggttccc gctgcgtgaa gccaccgatt
    11701 gttattggtg acgttagtcg cccggcaccg attaccgtgg agtgggcgaa gtatgcgcaa
    11761 tctatgactg ataaaccggt gaaagggatg ctgactggcc cggtgaccat tctctgctgg
    11821 tcgttcccgc gtgaagatgt cagccgtgaa accatcgcca aacaaattgc gctggcgctg
    11881 cgtgatgaag tggcggacct ggaagccgca ggaattggca tcatccagat tgatgaaccg
    11941 gcgctgcgcg aaggtttacc gctgcgtcgt agcgactggg atgcatatct ccagtggggc
    12001 gtggaggcct tccgtatcaa cgccgccgtg gcgaaagatg acacacaaat ccacactcac
    12061 atgtgttatt gcgagttcaa cgacatcatg gattcgattg cggcactgga cgcagacgtc
    12121 atcaccatcg aaacctcgcg ttctgacatg gagttgctgg agtcgttcga agagtttgat
    12181 tatccgaatg aaatcggtcc aggcgtctat gacattcact cgccaaacgt accgagcgtg
    12241 gaatggattg aagccttgct gaagaaagcg gcaaaacgca ttccggcaga gcgcctgtgg
    12301 gtcaacccgg actgtggcct gaaaacgcgc ggctggccag aaacccgcgc ggcactggcg
    12361 aacatggtgc aggcggcgca gaacttgcgt cgggggtaaa atccaaaccg ggtggtaata
    12421 ccacccggtc ttttctcatt acagcgactt cttcccacca tactgcttaa accattccag
    12481 catacgctgc cagccatctt ctgcagatgc ggcatgatag ctcgggcgat aatcagcgtt
    12541 gaatgcatgc ccggcgtccg ggtacacgat aatctctgct ttcgcattag cagcccgcag
    12601 cgcctggcgc atggtttcaa cgctctcctg cggaatactg ttatccagac cgccatataa
    12661 gccgagaacc ggcgcgttaa gatcggttgc gatatcaaca ggttgtttcg gtgaattcag
    12721 cgacttatcg cccgtcagtt tgccgtacca cgccactgcg gcttttagct gtggattatg
    12781 cgcggcatac agccaagtga tacgtccacc ccagcagaat ccggtgatca ttaaacgatg
    12841 aacatcgccg ccgttgcggg aagcccaact ggcgacatga tcgagatcgg ccagcacctg
    12901 cgagtcaggc acttttgcta ccagaccgct aagcaacgtg gggatatcgg caaaatcatt
    12961 cggatcgcct tcgcggaagt aaagttcagg tgcgatagcc agatacccct ccagcgccag
    13021 acggcgacaa atgtcgcgga tatgttcatg cacgccaaaa atttcctgca ctacaatgac
    13081 cactggcagt gggccatcgc tttgctttgg tctggcatgg taagcaggca tgttatcccc
    13141 ttgtgaaggg atagaggtga agcccgccac aatcgcgtcg tccggggtct gaacgatggt
    13201 cgaagcgaga ggcgatgcag caggtgcaaa tccagattgt tgtgttgttg ccatggtatt
    13261 ctccgtaccc ttataaaaat gttgcgcaat gttaactata gtcagcatgc aacaaatcac
    13321 attgcctgaa tcggctcatc ttttatgcag tcctgcagaa tgaagagtga tttatgtgat
    13381 ttgcatcacc tttggtgggt aaatttatgc aacgcatttg cgtcatggtg atgagtatca
    13441 cgaaaaaatg ttaaaccctt cggtaaagtg tctttttgct tcttctgact aaaccgattc
    13501 acagaggagt tgtatatgtc caagtctgat gtttttcatc tcggcctcac taaaaacgat
    13561 ttacaagggg ctacgcttgc catcgtccct ggcgacccgg atcgtgtgga aaagatcgcc
    13621 gcgctgatgg ataagccggt taagctggca tctcaccgcg aattcactac ctggcgtgca
    13681 gagctggatg gtaaacctgt tatcgtctgc tctaccggta tcggcggccc gtctacctct
    13741 attgctgttg aagagctggc acagctgggc attcgcacct tcctgcgtat cggtacaacg
    13801 ggcgctattc agccgcatat taatgtgggt gatgtcctgg ttaccacggc gtctgtccgt
    13861 ctggatggcg cgagcctgca cttcgcaccg ctggaattcc cggctgtcgc tgatttcgaa
    13921 tgtacgactg cgctggttga agctgcgaaa tccattggcg cgacaactca cgttggcgtg
    13981 acagcttctt ctgatacctt ctacccaggt caggaacgtt acgatactta ctctggtcgc
    14041 gtagttcgtc actttaaagg ttctatggaa gagtggcagg cgatgggcgt aatgaactat
    14101 gaaatggaat ctgcaaccct gctgaccatg tgtgcaagtc agggcctgcg tgccggtatg
    14161 gtagcgggtg ttatcgttaa ccgcacccag caagagatcc cgaatgctga gacgatgaaa
    14221 caaactgaaa gccaagcggt gaaaatcgtg gtggaagcgg cgcgtcgtct gctgtaattc
    14281 tcttctcctg tctgaaggcc gacgcgttcg gccttttgta tttttgcgta gcgcctcgca
    14341 ggaaatgcct ttccaactgg acgtttgtac agcacaattc tattttgtgc gggtaagttg
    14401 ttgcgtcagg aggcgttgtg gatttctcaa tcatggttta cgcagttatt gcgttggtgg
    14461 gtgtggcaat tggctggctg tttgccagtt atcaacatgc gcagcaaaaa gccgagcaat
    14521 tagctgaacg tgaagagatg gtcgcggagt taagcgcggc aaaacaacaa attacccaaa
    14581 gcgagcactg gcgtgcagag tgcgagttac tcaataacga agtgcgcagc ctgcaaagta
    14641 ttaacacctc tctggaggcc gatctgcgtg aagtaaccac gcggatggaa gccgcacagc
    14701 aacatgctga cgataaaatt cgccagatga ttaacagcga gcagcgcctc agtgagcagt
    14761 ttgaaaacct cgccaaccgt atttttgagc acagcaatcg ccgggttgat gagcaaaacc
    14821 gtcagagtct gaacagcctg ttgtcgccgc tacgtgaaca actggacggt ttccgccgtc
    14881 aggttcagga cagcttcggt aaagaagcac aagaacgcca taccctgacc cacgaaattc
    14941 gcaatctcca gcaactcaac gcgcaaatgg cccaggaagc gatcaacctg acgcgcgcgc
    15001 tgaaaggcga caataaaacc cagggcaact ggggcgaggt agtattgacg cgggtgctgg
    15061 aggcttccgg tctgcgtgaa gggtatgaat atgaaaccca ggtcagcatc gaaaatgacg
    15121 cccgctcgcg gatgcagccg gatgtcatcg tgcggctgcc gcagggaaaa gatgtggtga
    15181 tcgacgccaa aatgacgctg gtcgcctatg aacgctattt taacgccgaa gacgactaca
    15241 cccgcgaaag cgcgctacag gaacatatcg cgtcggtgcg taaccatatc cgtttgctgg
    15301 gacgcaaaga ttatcaacag ctgccggggc tgcgaactct ggattacgtg ctgatgttta
    15361 ttcccgttga acccgctttt ttactggcgc ttgaccgcca gccggagctg atcaccgaag
    15421 cgttgaaaaa caatatcatg ctggttagcc cgactacgct gctggtggcg ctgcgcacta
    15481 tcgccaacct gtggcgttat gagcatcaaa gccgcaacgc ccagcaaatc gccgatcgtg
    15541 ccagcaagct gtacgacaag atgcgtttgt tcatcgatga catgtccgcg attggtcaaa
    15601 gtctcgacaa agcgcaggat aactatcggc aggcaatgaa aaaactctct tcagggcgcg
    15661 gaaatgtgct ggcgcaggca gaagcgtttc gcggtttagg agtagaaatt aaacgcgaga
    15721 ttaatccgga tttggctgaa caggcggtga gccaggatga agagtatcga cttcggtcgg
    15781 ttccggagca gccgaatgat gaagcttatc aacgcgatga tgaatataat cagcagtcgc
    15841 gctagcccat tgggagtagt taagccgggt agaaatctag ggcatcgacg cccaatctgt
    15901 tacacttctg gaacaatttt ttgatgagca ggcattgaga tggtggataa gtcacaagaa
    15961 acgacgcact ttggttttca gaccgtcgcg aaggaacaaa aagcggatat ggtcgcccac
    16021 gttttccatt ccgtggcatc aaaatacgat gtcatgaatg atttgatgtc atttggtatt
    16081 catcgtttgt ggaagcgatt cacgattgat tgcagcggcg tacgccgtgg gcagaccgtg
    16141 ctggatctgg ctggtggcac cggcgacctg acagcgaaat tctcccgcct ggtcggagaa
    16201 actggcaaag tggtccttgc tgatatcaat gaatccatgc tcaaaatggg ccgcgagaag
    16261 ctgcgtaata tcggtgtgat tggcaacgtt gagtatgttc aggcgaacgc tgaggcgctg
    16321 ccgttcccgg ataacacctt tgattgcatc accatttcgt ttggtctgcg taacgtcacc
    16381 gacaaagata aagcactgcg ttcaatgtat cgcgtgctga aacccggcgg ccgcctgctg
    16441 gtgcttgagt tctcgaagcc aattatcgag ccgctgagca aagcctatga tgcatactcc
    16501 ttccatgtgc tgccgcgtat tggctcactg gtcgcgaacg acgccgacag ctaccgttat
    16561 ctggcagaat ccatccgtat gcatcccgat caggataccc tgaaagccat gatgcaggat
    16621 gccggattcg aaagtgtcga ctactacaat ctgacggcag gggttgtggc gctgcatcgt
    16681 ggttataagt tctgacagga gaccggaaat gccttttaaa cctttagtga cggcaggaat
    16741 tgaaagtctg ctcaacacct tcctgtatcg ctcacccgcg ctgaaaacgg cccgctcgcg
    16801 tctgttgggc aaagtgctgc gcgtggaggt aaaaggcttt tcgacgtcat tgattctggt
    16861 gttcagcgaa cgccaggttg atgtactggg cgaatgggca ggcgatgctg actgcaccgt
    16921 tatcgcctac gccagtgtgt tgccgaaact tcgcgatcgc cagcagcttg ccgccctgat
    16981 tcgcagtggt gagctggaag tgcagggcga tattcaggtg gtgcaaaact tcgttgcgct
    17041 ggcaggtctg gcagagttcg accctgcgga actgctggcc ccttataccg gtgatatcgc
    17101 cgctgaagga atcagcaaag ccatgcgcgg aggcgcaaag ttcctgcatc acggcattaa
    17161 gcgccagcaa cgttatgtgg cggaagccat tactgaagag tggcgtatgg cacccggtcc
    17221 gcttgaagtg gcctggtttg cggaagagac ggctgccgtc gagcgtgctg ttgatgccct
    17281 gatcaaacgg ctggaaaaac tggaggctaa atgacgccag gtgaagtacg gcgcctatat
    17341 ttcatcattc gcactttttt aagctacgga cttgatgaac tgatccccaa aatgcgtatc
    17401 accctgccgc tacggctatg gcgatactca ttattctgga tgccaaatcg gcataaagac
    17461 aaacttttag gtgagcgact acgactggcc ctgcaagaac tggggccggt atggatcaag
    17521 ttcgggcaaa tgttatcaac ccgccgcgat ctttttccgc cgcatattgc cgatcagctg
    17581 gcgttattgc aggacaaagt tgctccgttt gatggcaagc tggcgaagca gcagattgaa
    17641 gctgcaatgg gcggcttgcc ggtagaagcg tggtttgacg attttgaaat caagccactg
    17701 gcttctgctt ctatcgccca ggttcatacc gcgcgattga aatcgaatgg taaagaggtg
    17761 gtgattaaag tcatccgccc ggatattttg ccggtcatta aagcagacct gaaacttatc
    17821 taccgtctgg ctcgctgggt gccgcgtttg ctgccggatg gtcgccgtct acgcccaacc
    17881 gaagtggtgc gcgagtacga aaagaccttg attgatgaac tgaatttgct gcgggaatct
    17941 gccaacgcca ttcagcttcg gcgaaatttt gaagacagcc cgatgctcta catcccggag
    18001 gtttaccctg actattgtag tgaagggatg atggtgatgg agcgtattta cggcattccg
    18061 gtgtctgatg ttgcggcgct ggagaaaaac ggcaccaaca tgaaattgct ggcggaacgc
    18121 ggcgtgcagg tgttcttcac tcaggtcttt cgcgacagct ttttccatgc cgatatgcac
    18181 cctggcaaca tcttcgtaag ctatgaacac ccggaaaacc cgaaatatat cggcattgat
    18241 tgcgggattg ttggctcgct aaacaaagaa gataaacgct atctggcaga aaactttatt
    18301 gccttcttta atcgcgacta tcgcaaagtg gcagagctac acgtcgattc tggctgggtg
    18361 ccaccagata ccaacgttga agagttcgaa tttgctattc gtacggtctg tgaacctatc
    18421 tttgagaaac cgctggccga aatttcgttt ggacatgtac tgttaaatct gtttaatacg
    18481 gcgcgtcgct tcaatatgga agtgcagccg caactggtgt tactccagaa aaccctgctc
    18541 tacgtcgaag gggtaggacg ccagctttat ccgcaactcg atttatggaa aacggcgaag
    18601 cctttcctgg agtcgtggat taaagatcag gtcggtattc ctgcgctggt gagagcattt
    18661 aaagaaaaag cgccgttctg ggtcgaaaaa atgccagaac tgcctgaatt ggtttacgac
    18721 agtttgcgcc agggcaagta tttacagcac agtgttgata agattgcccg cgagcttcag
    18781 tcaaatcatg tacgtcaggg acaatcgcgt tattttctcg gaattggcgc tacgttagta
    18841 ttaagtggca cattcttgtt ggtcagccga cctgaatggg ggctgatgcc cggctggtta
    18901 atggcaggtg gtctgatcgc ctggtttgtc ggttggcgca aaacacgctg attttttcat
    18961 cgctcaaggc gggccgtgta acgtataatg cggctttgtt taatcatcat ctaccacaga
    19021 ggaacatgta tgggtggtat cagtatttgg cagttattga ttattgccgt catcgttgta
    19081 ctgctttttg gcaccaaaaa gctcggctcc atcggttccg atcttggtgc gtcgatcaaa
    19141 ggctttaaaa aagcaatgag cgatgatgaa ccaaagcagg ataaaaccag tcaggatgct
    19201 gattttactg cgaaaactat cgccgataag caggcggata cgaatcagga acaggctaaa
    19261 acagaagacg cgaagcgcca cgataaagag caggtgtaat ccgtgtttga tatcggtttt
    19321 agcgaactgc tattggtgtt catcatcggc ctcgtcgttc tggggccgca acgactgcct
    19381 gtggcggtaa aaacggtagc gggctggatt cgcgcgttgc gttcactggc gacaacggtg
    19441 cagaacgaac tgacccagga gttaaaactc caggagtttc aggacagtct gaaaaaggtt
    19501 gaaaaggcga gcctcactaa cctgacgccc gaactgaaag cgtcgatgga tgaactacgc
    19561 caggccgcgg agtcgatgaa gcgttcctac gttgcaaacg atcctgaaaa ggcgagcgat
    19621 gaagcgcaca ccatccataa cccggtggtg aaagataatg aagctgcgca tgagggtgta
    19681 acgcctgccg ctgcacaaac gcaggccagt tcgccggaac agaagccaga aaccacgcca
    19741 gagccggtgg taaaacctgc tgcggacgct gaaccgaaaa ccgctgcacc ttccccttcg
    19801 tcgagtgata aaccgtaaac atgtctgtag aagatactca accgcttatc acgcatctga
    19861 ttgagctgcg taagcgtctg ctgaactgca ttatcgcggt gatcgtgata ttcctgtgtc
    19921 tggtctattt cgccaatgac atctatcacc tggtatccgc gccattgatc aagcagttgc
    19981 cgcaaggttc aacgatgatc gccaccgacg tggcctcgcc gttctttacg ccgatcaagc
    20041 tgacctttat ggtgtcgctg attctgtcag cgccggtgat tctctatcag gtgtgggcat
    20101 ttatcgcccc agcgctgtat aagcatgaac gtcgcctggt ggtgccgctg ctggtttcca
    20161 gctctctgct attttatatc ggcatggcgt tcgcctactt tgtggtcttt ccgctggcat
    20221 ttggcttcct tgccaatacc gcgccggaag gggtgcaggt atccaccgac atcgccagct
    20281 atttaagctt cgttatggcg ctgtttatgg cgtttggtgt ctcctttgaa gtgccggtgg
    20341 caattgtgct gctgtgctgg atggggatta cctcgccaga agacttacgc aaaaaacgcc
    20401 cgtatgtgct ggttggtgca ttcgttgtcg ggatgttgct gacgccgccg gatgtcttct
    20461 cgcaaacgct gttggcgatc ccgatgtact gtctgtttga aatcggtgtc ttcttctcac
    20521 gcttttacgt tggtaaaggg cgaaatcggg aagaggaaaa cgacgctgaa gcagaaagcg
    20581 aaaaaactga agaataaatt caaccgcccg tcagggcggt tgtcatatgg agtacaggat
    20641 gtttgatatc ggcgttaatt tgaccagttc gcaatttgcg aaagaccgtg atgatgttgt
    20701 agcgcgcgct tttgacgcgg gagttaatgg gctactcatc accggcacta acctgcgtga
    20761 aagccagcag gcgcaaaagc tggcgcgtca gtattcgtcc tgttggtcaa cggcgggcgt
    20821 acatcctcac gacagcagcc agtggcaagc tgcgactgaa gaagcgatta ttgagctggc
    20881 cgcgcagcca gaagtggtgg cgattggtga atgtggtctc gactttaacc gcaacttttc
    20941 gacgccggaa gagcaggaac gcgcttttgt tgcccagcta cgcattgccg cagatttaaa
    21001 catgccggta tttatgcact gtcgcgatgc ccacgagcgg tttatgacat tactggagcc
    21061 gtggctggat aaactgcctg gtgcggttct tcattgcttt accggcacac gcgaagagat
    21121 gcaggcgtgc gtggcgcatg gaatttatat cggcattacc ggttgggttt gcgatgaacg
    21181 acgcggactg gagctgcggg aacttttgcc gttgattccg gcggaaaaat tactgatcga
    21241 aactgatgcg ccgtatctgc tccctcgcga tctcacgcca aagccatcat cccggcgcaa
    21301 cgagccagcc catctgcccc atattttgca acgtattgcg cactggcgtg gagaagatgc
    21361 cgcatggctg gctgccacca cggatgctaa tgtcaaaaca ctgtttggga ttgcgtttta
    21421 gagtttgcgg aactcggtgt tcttcacact gtgcttaatc tctttattaa taagattaag
    21481 caatagcatg gagcgagcct caccatcggg ttcggtgaaa atggcctgaa agccttcgaa
    21541 cgcgccttcg gtaataatca ccttatcacc cggataaggg gttgccggat cgacaatgtc
    21601 tttcggttta tataccgata gctgatgaat caccgccgat gggactatcg ctggcgacgc
    21661 gccaaagcgc acgaagtggc tgacaccgcg ggtcgcgttg atagtcgtgg tatgaatcac
    21721 ttctggatca aattccacaa acaggtagtt ggggaacaat ggctcactga ctgcagtacg
    21781 tttgccacgc acgatttttt ccagggtgat catcggtgcc aggcaattca cagcctgtct
    21841 ttcgaggtgt tcctgggcac gttgaagttg cccgcgcttg cagtacagta aataccagga
    21901 ttgcataatg actcttatcc gtttaatcgg ggcgcaagga tagcaaaagc tttacgctaa
    21961 gttaattata ttccccggtt tgcgttatac cgtcagagtt cacgctaatt taacaaattt
    22021 acagcatcgc aaagatgaac gccgtataat gggcgcagat taagaggcta caatggacgc
    22081 catgaaatat aacgatttac gcgacttctt gacgttgctt gaacagcagg gtgagctaaa
    22141 acgtatcacg ctcccggtgg acccgcatct ggaaatcact gaaattgctg accgcacgct
    22201 gcgtgctggt gggcctgcgc tgttgtttga aaaccctaaa gggtactcaa tgccggtgct
    22261 gtgcaacttg ttcggtacgc caaagcgcgt agcgatgggt atgggccagg aagatgtttc
    22321 agcactgcgt gaagtcggta aattattagc atttctgaaa gaaccagagc cgccaaaagg
    22381 ttttcgcgat ctgtttgata agctgccgca gtttaagcag gtgttaaaca tgccgacaaa
    22441 gcgactgcgc ggtgcaccct gccaacaaaa aatcgtctct ggcgatgacg tcgatctcaa
    22501 ccgtattccc attatgacct gttggccgga agatgccgcg ccgctgatta catgggggct
    22561 aaccgttaca cgtggccctc ataaagagcg acagaatctg ggcatttatc gccagcaact
    22621 gattggtaaa aacaagctga ttatgcgttg gctgtcgcat cgcggcggcg cgctggatta
    22681 tcaggagtgg tgtgcggcgc atccaggtga acgtttcccg atctctgtgg cgttgggcgc
    22741 tgatccggca accattctcg gtgcagtcac accagtacca gatactttgt cggaatacgc
    22801 ctttgccgga ttgctacgtg gcaccaaaac cgaagtagtg aagtgtattt ccaatgatct
    22861 cgaagtgccc gccagtgcgg agattgtgct ggaagggtat atcgaacaag gcgaaatggc
    22921 gccagaagga ccgtatggtg accacactgg ttactataac gaagtcgata gtttcccggt
    22981 atttaccgtg acgcatatta cccagcgtga agatgcgatt taccattcca cctataccgg
    23041 gcgtccgcca gatgaacccg cggtactggg agtggcgttg aacgaagtat ttgttcccat
    23101 tctgcaaaag cagttcccgg aaattgtcga tttttacctg ccgccggaag gctgctctta
    23161 tcgcctggcg gtagtgacaa tcaaaaaaca gtacgccgga cacgcgaagc gcgtcatgat
    23221 gggcgtctgg tcgttcttac gccagtttat gtacactaaa tttgtgatcg tttgcgatga
    23281 tgacgttaac gcacgcgact ggaacgatgt gatttgggcg attaccaccc gtatggaccc
    23341 agcgcgggat actgttctgg tagaaaatac gcctattgat tatctggatt ttgcctcgcc
    23401 tgtctccggg ctgggttcaa aaatggggct ggatgccacg aataaatggc cgggggaaac
    23461 ccagcgtgaa tggggacgtc ccatcaaaaa agatccagat gttgtcgcac atattgacgc
    23521 catctgggat gaactggcta tttttaacaa cggtaaaagc gcctgatgcg cgtttgtttt
    23581 gccctattta tcgatccgac agagaaagcg catgacaacc ttaagctgta aagtgacctc
    23641 ggtagaagct atcacggata ccgtatatcg tgtccgcatc gtgccagacg cggccttttc
    23701 ttttcgtgct ggtcagtatt tgatggtagt gatggatgag cgcgacaaac gtccgttctc
    23761 aatggcctcg acgccggatg aaaaagggtt tatcgagctg catattggcg cttctgaaat
    23821 caacctttac gcgaaagcag tcatggaccg catcctcaaa gatcatcaaa tcgtggtcga
    23881 tattccccac ggagaagcgt ggctgcgcga tgatgaagag cgtccgatga ttttgattgc
    23941 gggcggcacc gggttctctt atgcccgctc gattttgctg acagcgttgg cgcgtaaccc
    24001 aaaccgtgat atcaccattt actggggcgg gcgtgaagag cagcatctgt atgatctctg
    24061 cgagcttgag gcgctttcgt tgaagcatcc tggtctgcaa gtggtgccgg tggttgaaca
    24121 accggaagcg ggctggcgtg ggcgtactgg caccgtgtta acggcggtat tgcaggatca
    24181 cggtacgctg gcagagcatg atatctatat tgccggacgt tttgagatgg cgaaaattgc
    24241 ccgtgacctg ttttgcagtg agcgtaatgc gcgggaagat cgcctgtttg gcgatgcgtt
    24301 tgcatttatc tgagatataa aaaaacccgc ccctgacagg cgggaagaac ggcaactaaa
    24361 actttattca gtggcattta gatctatgtc gcatccggca ttcggcacac gatgcctgat
    24421 gcgacgctgg cgcgtcttat caggcctaca ttggtgccgg atcggtagac cggataaggc
    24481 gttcacgccg catccggcaa gtggttaaac ccgctcaaac accgtcgcaa taccctgacc
    24541 cagaccgata cacatcgtcg ccagaccaaa ctgaacgtct ttgtgttcca tcagattcag
    24601 cagcgtggtg ctgatacgcg caccggaaca acccagcgga tgacccagcg cgatcgcgcc
    24661 accgttgagg ttgatcttct cgtcaatctg ctcaattagt cccagatctt taatacatgg
    24721 caggatctgc gcggcaaagg cttcattcat ttcaaacacg ccgatatcac tgacagaaag
    24781 ccccgctttt ttcagcgcca gcttcgaggc cggaaccggg ccgtaaccca tgatcgatgg
    24841 gtcacaacca acgaccgcca tcgaacgcac acgagcgcgc ggcttaagac ctaattcatg
    24901 ggcgcggctt tcactcatca ccagcatggc agctgcgcca tcggaaagtg cagaagatgt
    24961 gcccgccgtt accgtaccgt ttactggatc aaacgccgga cgcagcgtgg cgagggcttc
    25021 cacggtggtt tccgggcgaa tcacttcgtc gtaattaaac tgcttcagga cgccgtcggc
    25081 atcgtgacca ccagttggga tgatttcatt tttaaatgcg cccgactgcg tggcggccca
    25141 ggcgcgggcg tgtgaccgcg cggcaaaggc atcctgcatt tcacggctga taccgtgcat
    25201 acgcgccagc atttctgccg ttaagcccat catgcccgcc gctttggcga cattgcggct
    25261 caggccaggg tgaaaatcga cgccgtgact catcggcaca tggcccatat gctccacgcc
    25321 gccaaccaga catgcctgcg catcgccggt catgatcatt cgtgctgcgt catgcagtgc
    25381 ctgcatggat gaaccacaca agcgattgac agtaaccgcc gggacagagt gcggcacttc
    25441 tgccagcagc gccgcgttac gggcgatatt aaaaccctgc tccagcgtct gctgcacaca
    25501 cccccagtaa atatcgtcga gggccgccgc ttccagcgcc gggttacgcg ccagcaggct
    25561 acgcattaaa tgagcggaga gatcttctgc acgcacgtta cgaaaagcac cgcccttcga
    25621 acggcccatc ggggtgcgaa ttgcatcgac aatgacaacc tgttccattg tgactcctta
    25681 agccgttttc aggtcgccaa ccggacgggc tggctcaacc gggggatagt acggttcgtt
    25741 atgacgcgct ttattacgca gaccttccgg cacttcgtac agcgggccga ggtgctgata
    25801 ttgctgtgcc atatcgaggt attttgcgct gccgagggtg tccagccagc ggaacgcgcc
    25861 gccgtggaac ggagggaagc ccaggccgta gaccagcgcc atatccgctt ctgccggagt
    25921 ggcgataatg ccttcttcca gacagcgcac cacttcgttg accatcggga tcatcatgcg
    25981 ggcgataatc tcttcttcgc tgaaatcgcg cttcggctgg ctcacttctg ccagcaggtc
    26041 gtcaacagcg gcgtcttctt ctttcttcgg cttacctttg ctgtcttctt tataatgcca
    26101 gaaaccgagg ccgttcttct gaccaaagcg gttggcatca aacagcgcgt cgatggcatc
    26161 gcgataatct ttctgcatcc gctgtgggaa gcccgctgcc atgactgcct gggcgtgatg
    26221 cgcggtgtca atgcccacaa cgtccagcag atatgccgga cccataggcc agccaaactg
    26281 tttttccatc actttgtcga tcttgcggaa atccgcgccg tcgcgcagca actggctgaa
    26341 accggcgaaa tacgggaaca gcacgcggtt gacgaagaag ccggggcaat cgttaaccac
    26401 aatcggcgtt ttacccatct tgctcgccca ggcgacgact ttcgcaatag tttcgtcgga
    26461 gcttttctcg ccgcgaataa tttccaccaa cggcattcgg tggaccgggt taaagaagtg
    26521 catcccgcag aagttttccg gacgttccag cgcgttggcc agttcgctga taggaatggt
    26581 tgaagtgtta gacgccagca cggtatccgg gcgtactttc tgctcggttt ctgccagcac
    26641 ggcttttttc actttcgggt tttcaacaat cgcttctacc acaacatcca cgcgctcaaa
    26701 tccggcgtag tcgagcgttg ggtggatcgt ggagatcacg ccagccagtt tcagaccatc
    26761 gatcttgccg cgctcaagct gcttgttcag tagtttcgcg gcttcagtca tgccgagggc
    26821 taacgacttg tcgttaatat ctttcataat caccggcacg cctttccacg cagactggta
    26881 agcgatgccg ccacccataa tgcctgcgcc cagcacggcg gcctgtttcg gggtttcaac
    26941 gtctttggtg agtttcttcg ctttgccttt tacatattga tcgttaagga aaatgccgac
    27001 cagtgcgcga gcttcgttgg tatgcgccag cgggacaaag cttttgtttt ctaggtttaa
    27061 ggcttcttca cgaccaaaac gagccgcagc ttcaatggtt tttactgcgg tgatgggggc
    27121 cgggtaatgt tttcctgccg tttgtgcgac catcccttta gcgatggtga agctcatggt
    27181 ggcttcaatc ttgctcagtt ttagcggttc cagcttcggc tgacgtttcg ctttccagtc
    27241 gaggtcgccg ttaatggcct ggcgtaaaat cgccatcgcc ccttcaatca gtttttctgc
    27301 tttgactacg ccatccacca gaccgatttt cagcgcctga tccgcgccga catctttacc
    27361 ggcggcaatg atttccagcg cactgtcagc gcccagcata cgtggcatac gtacagaacc
    27421 gccaaagccc ggcatgatgc ccagtttggt ttccggcaga ccgatgcgca gatccggcgt
    27481 cgccagacgg taatcggtcg ccagcacgca ttcgcagcca ccgcccagcg catagccatt
    27541 gacggcagca atggtcggca ccggcagatc ttccaggcga ttaaacacgc tattggcaaa
    27601 gtgcagccac tgacttaact gttcttcagg aacgaggaac agggacaaaa attcggtgat
    27661 atcagcaccg acgataaagg ctgctttgtt cgaacgcagc agcagccctt ttagatctga
    27721 ttgctgttcc agcacgccga tggcctcgcc gaggctggcg acggtcgcag tgtcgagttt
    27781 attaactgaa cctggggcat caaataccag ttcggcaatg ccatcttcca gccagtcaag
    27841 gtacagggtg tcgcctttgt aaagcatgtc agtctcctga atccgcaagg tgatctggtc
    27901 gtaccagatg catcgaagtg tgtattttgt gttaaaaata tgcaaataaa agattaaaga
    27961 aatgccgatc tgatcacgct cggcagaaat cacgctctgg atgaacgatg tgctaagatg
    28021 cggagactta aggtcaaaaa aacagaaggg taaaaaatgg aatcactggc ctcgctctat
    28081 aaaaatcata tagctacatt gcaggaacga actcgcgatg cgctgacgcg cttcaagctg
    28141 gatgcgttac ttattcactc cggcgaactg ttcaacgttt ttctcgacga tcatccctat
    28201 ccgtttaaag tgaacccgca attcaaagcg tgggtgccgg taactcaggt gccaaactgc
    28261 tggctgctgg tggatggcgt gaataagccg aaactgtggt tctatctgcc ggttgattac
    28321 tggcacaacg tcgaaccgct gccgacctcc ttctggactg aagatgtaga agtgatcgcg
    28381 ctgccgaaag ccgatggcat tggtagtctg ttgcctgctg cgcgcggcaa tatcggttat
    28441 atcggtccgg tgccggaacg tgcgctgcaa ctgggtattg aggccagcaa tatcaacccg
    28501 aaaggggtta tcgactacct gcattactac cgctccttca aaaccgagta cgagctggcc
    28561 tgtatgcgtg aagcgcagaa aatggcggtc aacggtcatc gcgcggcaga agaagcgttc
    28621 cgttctggca tgagcgagtt cgatatcaat attgcctatc tgactgcgac cggtcatcgt
    28681 gataccgacg taccttacag caacattgtg gcactcaacg aacacgctgc ggtgctgcat
    28741 tacaccaaac tggatcatca ggcgtcggaa gagatgcgca gcttcctgct ggatgccggg
    28801 gccgaatata acggctatgc cgctgacctg acccgtacct ggtcggcaaa aagtgacaac
    28861 gattacgcac agctggtgaa agacgtaaat gatgaacaac tggcgctgat cgcgaccatg
    28921 aaagctggcg ttagctatgt ggattaccac atccagttcc atcagcgcat cgccaaattg
    28981 ctgcgtaaac atcaaatcat caccgatatg agtgaagagg cgatggtcga aaacgatctt
    29041 accgggccgt ttatgccgca tggtatcggc catccgctgg gcctgcaggt gcatgacgtc
    29101 gccggtttta tgcaggatga tagcggtacg cacctcgcgg caccggcaaa atatccgtac
    29161 ctgcgctgca cccgtattct ccagccgggc atggtgttaa ccatcgaacc gggtatctac
    29221 ttcattgaat cgctgctggc accgtggcgt gaagggcagt tcagcaagca cttcaactgg
    29281 cagaaaattg aagcactgaa accgttcggc ggcattcgta tcgaagacaa cgtggtgatc
    29341 cacgaaaata acgtggaaaa catgacccgg gatctgaaac tggcgtgatg gaaagctggt
    29401 taattcctgc ggcaccggtc acggtcgttg aagagatcaa aaaaagccgc ttcattacgc
    29461 tgttggcgca taccgatggc gttgaggcgg cgaaagcgtt tgttgaatcg gtgcgggcag
    29521 aacaccccga tgcccgccac cattgcgtgg cgtgggtggc gggtgcgccg gatgattctc
    29581 aacagctggg tttctctgac gacggggagc cggcgggaac ggcaggtaaa ccgatgctcg
    29641 cccagctaat gggcagcggc gttggggaaa ttaccgctgt ggtggtgcgc tactacggcg
    29701 gcatattgct tggcaccggt gggttagtga aagcgtatgg cggcggcgtg aatcaggcgc
    29761 tgcgccagct aacgacccaa cgcaagacgc cattaaccga atatactttg caatgtgaat
    29821 atagtcagtt aaccggcatt gaagcgttgc tggggcagtg tgacggcaaa attatcaaca
    29881 gtgattatca ggcattcgtt ctgctgcggg tggcgcttcc ggcggcgaaa gtggctgaat
    29941 tttccgcgaa gctggcggat tttagccgtg gttcattgca attgttagcg attgaagaat
    30001 aatccccact tcgttttgca gaactaagga agcggcagag atgcattttc gcgccattac
    30061 ccgaatcgtt ggactactgg tcatcttatt ttcagggacc atgattatcc ctgggctggt
    30121 agcactcatc taccgggatg gagcgggccg cgcttttacc cagacctttt ttgtcgccct
    30181 cgccattggc tctatgctgt ggtggccgaa ccgcaaagag aagggcgaac tgaaatcccg
    30241 tgaggggttt ctgatagtgg tgctgttctg gaccgtgctg ggtagtgtcg gtgcgctccc
    30301 ttttatcttc tcggaaagcc cgaacctcac gattaccgat gcgttttttg aatctttctc
    30361 tggcctgacc accaccgggg ccactacgct ggtggggctg gattcgctcc ctcatgccat
    30421 cctcttttat cgccagatgc tgcaatggtt tggcgggatg gggatcatcg tgttagcggt
    30481 tgcgatactg cctatcctcg gcgtgggtgg gatgcagctc tatcgcgcag aaatgcccgg
    30541 cccgctgaaa gataacaaaa tgcgcccgcg aattgcggaa acggcgaaaa ccctgtggtt
    30601 gatttatgtc ttgctgaccg tcgcctgtgc gctggcgttg tggtttgccg gaatggatgc
    30661 ctttgatgcc atcggccata gctttgcgac tatcgctatt ggcggcttct cgacacatga
    30721 tgccagtatc ggttatttcg atagcccgac tattaacact atcattgcta tcttcctgct
    30781 gatctccggc tgtaactacg gtctgcactt ttcactgtta agtgggcgta gtctgaaggt
    30841 ttattggcgc gatccggaat ttcgcatgtt tatcggcgta cagtttacgc tggtggttat
    30901 ttgtaccctc gtactgtggt ttcataatgt ctacagttcg gcgctgatga caattaacca
    30961 ggcgtttttc caggtggtgt cgatggcgac aaccgccggg tttacgactg acagcattgc
    31021 ccgctggccg ctctttttgc cggtactgct tttatgttca gcttttatcg gcggttgtgc
    31081 cgggtcaacg ggcggtggcc tgaaagtgat ccgcatcctg ctgctgttta agcaggggaa
    31141 ccgtgaactg aaacgactgg tgcatccgaa cgccgtctat agcattaagc tggggaatcg
    31201 cgcactgccg gaacgtatcc tcgaagccgt ttggggattt ttctccgcct atgcattggt
    31261 gtttattgtc agtatgctgg cgattatcgc cacgggcgtg gatgactttt ctgcctttgc
    31321 ctcggttgtt gcgacattga ataacctggg gccagggctt ggcgtggttg ctgataactt
    31381 taccagtatg aacccggtgg ctaaatggat cctgattgcc aacatgctgt ttggtcgtct
    31441 cgaggtcttt acattgctgg tgctctttac cccgactttc tggcgtgaat gatggagtaa
    31501 tacgtgaaaa cattaattct tttctcaaca agggacggac aaacgcgcga gattgcctcc
    31561 tacttggctt cggaactgaa agaactgggg atccaggcgg atgtcgccaa tgtgcaccgc
    31621 attgaagaac cacagtggga aaactacgac cgtgtggtca ttggtgcttc tattcgctat
    31681 ggtcactacc actctgcgtt ccaggaattt gtcaaaaagc atgcgacgcg gctgaattcg
    31741 atgccgagcg cgttttactc cgtaaacctg gtggcgcgta aaccggagaa gcgcactcca
    31801 cagaccaaca gctacgcgcg gaagtttctg atgaactcgc aatggcgtcc cgatcgctgc
    31861 gcggtcattg ccggggcgct gcgttaccca cgttatcgct ggtacgaccg ttttatgatc
    31921 aagctgatta tgaagatgtc aggcggtgaa acggatacgc gcaaagaagt tgtctatacc
    31981 gattgggagc aggtggcgaa tttcgcccga gaaatcgccc atttaaccga caaaccgacg
    32041 ctgaaataag cataaagaat aaaaaatgcg cggtcagaaa attattttaa atttcc
//
LOCUS       DAZKIF010000048        31110 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZKIF010000048 DAZKIF010000000
VERSION     DAZKIF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.048"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1489
                     /locus_tag="ACHOTW_002166"
     CDS             <1..1489
                     /locus_tag="ACHOTW_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFP9844275.1"
                     /translation="YLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELINTLAFLPDSTD
                     QIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESN
                     NNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLL
                     LCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSI
                     RDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEA
                     LCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLH
                     INVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQG
                     LRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPV
                     IAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            1497..2825
                     /locus_tag="ACHOTW_002167"
     CDS             1497..2825
                     /locus_tag="ACHOTW_002167"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9844276.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(2895..3224)
                     /locus_tag="ACHOTW_002168"
     CDS             complement(2895..3224)
                     /locus_tag="ACHOTW_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9844277.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(3214..3600)
                     /locus_tag="ACHOTW_002169"
     CDS             complement(3214..3600)
                     /locus_tag="ACHOTW_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9844278.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGSKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            complement(3826..5151)
                     /gene="rimO"
                     /locus_tag="ACHOTW_002170"
     CDS             complement(3826..5151)
                     /gene="rimO"
                     /locus_tag="ACHOTW_002170"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP9844279.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            5364..5747
                     /gene="bssR"
                     /locus_tag="ACHOTW_002171"
     CDS             5364..5747
                     /gene="bssR"
                     /locus_tag="ACHOTW_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP9844280.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            5858..6973
                     /gene="yliI"
                     /locus_tag="ACHOTW_002172"
     CDS             5858..6973
                     /gene="yliI"
                     /locus_tag="ACHOTW_002172"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP9844281.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(6970..7596)
                     /gene="gstB"
                     /locus_tag="ACHOTW_002173"
     CDS             complement(6970..7596)
                     /gene="gstB"
                     /locus_tag="ACHOTW_002173"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP9844282.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            7842..9044
                     /gene="dacC"
                     /locus_tag="ACHOTW_002174"
     CDS             7842..9044
                     /gene="dacC"
                     /locus_tag="ACHOTW_002174"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9844283.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(9091..9849)
                     /gene="deoR"
                     /locus_tag="ACHOTW_002175"
     CDS             complement(9091..9849)
                     /gene="deoR"
                     /locus_tag="ACHOTW_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP9844284.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(9907..10503)
                     /gene="ybjG"
                     /locus_tag="ACHOTW_002176"
     CDS             complement(9907..10503)
                     /gene="ybjG"
                     /locus_tag="ACHOTW_002176"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9844285.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            10788..12020
                     /gene="mdfA"
                     /locus_tag="ACHOTW_002177"
     CDS             10788..12020
                     /gene="mdfA"
                     /locus_tag="ACHOTW_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFP9844286.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(12061..12345)
                     /gene="ybjH"
                     /locus_tag="ACHOTW_002178"
     CDS             complement(12061..12345)
                     /gene="ybjH"
                     /locus_tag="ACHOTW_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP9844287.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(12431..13246)
                     /gene="ybjI"
                     /locus_tag="ACHOTW_002179"
     CDS             complement(12431..13246)
                     /gene="ybjI"
                     /locus_tag="ACHOTW_002179"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP9844288.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(13246..14454)
                     /locus_tag="ACHOTW_002180"
     CDS             complement(13246..14454)
                     /locus_tag="ACHOTW_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9844289.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14538..15074
                     /gene="rcdA"
                     /locus_tag="ACHOTW_002181"
     CDS             14538..15074
                     /gene="rcdA"
                     /locus_tag="ACHOTW_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP9844290.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(15249..16934)
                     /locus_tag="ACHOTW_002182"
     CDS             complement(15249..16934)
                     /locus_tag="ACHOTW_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP9844291.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            17204..17581
                     /locus_tag="ACHOTW_002183"
     CDS             17204..17581
                     /locus_tag="ACHOTW_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP9844292.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(17611..17868)
                     /gene="grxA"
                     /locus_tag="ACHOTW_002184"
     CDS             complement(17611..17868)
                     /gene="grxA"
                     /locus_tag="ACHOTW_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP9844293.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            18028..18315
                     /locus_tag="ACHOTW_002185"
     CDS             18028..18315
                     /locus_tag="ACHOTW_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP9844294.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            18299..19021
                     /gene="nfsA"
                     /locus_tag="ACHOTW_002186"
     CDS             18299..19021
                     /gene="nfsA"
                     /locus_tag="ACHOTW_002186"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP9844295.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            19082..19984
                     /gene="rimK"
                     /locus_tag="ACHOTW_002187"
     CDS             19082..19984
                     /gene="rimK"
                     /locus_tag="ACHOTW_002187"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP9844296.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            20072..20548
                     /locus_tag="ACHOTW_002188"
     CDS             20072..20548
                     /locus_tag="ACHOTW_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP9844297.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            20899..22011
                     /gene="potF"
                     /locus_tag="ACHOTW_002189"
     CDS             20899..22011
                     /gene="potF"
                     /locus_tag="ACHOTW_002189"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP9844298.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            22106..23239
                     /gene="potG"
                     /locus_tag="ACHOTW_002190"
     CDS             22106..23239
                     /gene="potG"
                     /locus_tag="ACHOTW_002190"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP9844299.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            23249..24202
                     /gene="potH"
                     /locus_tag="ACHOTW_002191"
     CDS             23249..24202
                     /gene="potH"
                     /locus_tag="ACHOTW_002191"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP9844300.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            24199..25044
                     /gene="potI"
                     /locus_tag="ACHOTW_002192"
     CDS             24199..25044
                     /gene="potI"
                     /locus_tag="ACHOTW_002192"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP9844301.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            25104..25592
                     /locus_tag="ACHOTW_002193"
     CDS             25104..25592
                     /locus_tag="ACHOTW_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP9844302.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            25633..26760
                     /gene="rlmC"
                     /locus_tag="ACHOTW_002194"
     CDS             25633..26760
                     /gene="rlmC"
                     /locus_tag="ACHOTW_002194"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP9844303.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            27040..28383
                     /locus_tag="ACHOTW_002195"
     CDS             27040..28383
                     /locus_tag="ACHOTW_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9844304.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            28405..28725
                     /locus_tag="ACHOTW_002196"
     CDS             28405..28725
                     /locus_tag="ACHOTW_002196"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9844305.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            28737..30218
                     /locus_tag="ACHOTW_002197"
     CDS             28737..30218
                     /locus_tag="ACHOTW_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFP9844306.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GENYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(30269..31000)
                     /gene="artJ"
                     /locus_tag="ACHOTW_002198"
     CDS             complement(30269..31000)
                     /gene="artJ"
                     /locus_tag="ACHOTW_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP9844307.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
BASE COUNT         7653 a         7370 c         7997 g         8090 t
ORIGIN      
        1 ttatctgccg tttgcgttca ttattatcgt attgctggtg gtttatttaa tgaccactcg
       61 tgtgttccgt cggcaatttt ctgaaatgac agagctgatt aatacgctgg cgtttttgcc
      121 tgactcaacg gatcagatcg aggctctgaa aattcgcgaa ggcgatgcga aagagattat
      181 cagcatcaaa aattcgatcg cggaaatgaa agatgccgaa attgaacggt caaataaatt
      241 gctctcactg atctcttacg atcaggaaag tggttttatt aaaaatatgg cgattattga
      301 gtccaacaat aatcagtatc tggctgtggg gatcatcaaa ctgtgtggtc tggaagccgt
      361 ggaagctgtg tttggtgttg atgaacgcaa taaaatcgtc aggaaattgt gtcagcgaat
      421 tgctgagaaa tatgcacaat gctgcgatat cgtgacattc aatgccgatc tctatttact
      481 tctgtgtcgg gaaaatgtac agacatttac ccgtaaaata gcgatggtaa acgattttga
      541 cagcagcttt ggctaccgca atctgcgcat ccataagtct gccatttgtg aacctttgca
      601 gggggaaaac gcctggagtt acgcagaaaa actgaaactg gcgatttcca gtatccgtga
      661 ccatatgttc tccgagttta ttttctgtga tgacgcgaaa ctcaacgaaa tagaagagaa
      721 tatctggatt gcgcgtaata ttcgccatgc aatggaaatt ggcgaactat tcctcgtcta
      781 tcaaccgatc gttgatatta acacccgcgc cattctgggc gcggaggcgt tgtgccgttg
      841 ggtgtctgcg gagcggggga tcatttcacc gctgaagttc attaccattg ctgaagatat
      901 cgggtttatc aatgagctgg gttatcagat tattaaaacg gcaatgggtg aattcagaca
      961 ttttagtcag cgtgcatcgc tgaaggatga tttcttactg catattaatg tttcgccctg
     1021 gcagttaaac gaaccgcact ttcatgagcg ttttaccacc atcatgaaag aaaatggcct
     1081 gaaggcgaac agcctctgtg ttgagatcac tgaaaccgtg atcgagcgaa ttaatgaaca
     1141 tttttatctc aatattgaac aactgcgtaa acaagggtta cggatatcga ttgatgactt
     1201 tggcaccggt ttgtcaaacc tgaaacgttt ttatgaaatt aatccagaca gcataaaggt
     1261 ggactcgcaa tttaccggcg atattttcgg tactgcggga aaaattgtgc gcattatttt
     1321 cgacctggca cgctataacc ggatcccggt gattgcggaa ggcgtagaga gcgaagacgt
     1381 tgcgcgcgaa ttaatcaaat taggatgtgt tcaggctcag gggtatctgt atcagaaacc
     1441 catgccattc tccgcctggg ataaaagtgg aaaattagta aaagagtagt ttacgtatgt
     1501 ccagaatcaa taagttcgta cttacagtca gtctgctgat ttttatcatg atttcagcag
     1561 ttgcctgcgg gatctacact caaatggtaa aggaacgggt gtatagcctg aaacagtccg
     1621 ttattgatac tgcttttgcg gtggcaaata ttgctgaata tcggcgtagc gtggcaattg
     1681 atcttatcaa cacgctaaat cccacggagg aacagctgtt ggttggctta cgcacagctt
     1741 acgccgactc ggtttcaccc tcttatttgt acgatgtcgg tccttatctg atttccagtg
     1801 acgaatgtat tcaggtaaag gagttcgaga aaaattattg tgcagatatt atgcaggttg
     1861 tgaagtatcg acatgtcaaa aatacagggt ttatctcttt tgacggtaaa accttcgtct
     1921 attacctcta tccggtaact cacaatcgta gtctgatatt tttgcttggt ctggagcgtt
     1981 tttctttact gtcaaaatcg ctggcgatgg acagcgagaa cctgatgttc tctctgttta
     2041 agaacggtaa accggtgacc ggtgatgaat ataatgctaa aaacgccatc ttcaccgttt
     2101 cggaagcgat ggagcacttc gcctatttgc cgaccggatt gtacgtattt gcgtataaaa
     2161 aagatgttta tttgcgggtc tgtacattga ttattttctt tgccgcattg gtggcagtga
     2221 tatcgggtgc cagttgcctc tatctggtac gcagagtgat taatcgtggt attgtggaga
     2281 aagaagccat tattaataac cattttgaac gcgtactgga tggcgggctt ttcttttcgg
     2341 ctgccgatgt caaaaaactc tacagtatgt ataactcggc gttcctggac gacctgacca
     2401 aagcaatggg tagaaaatcc tttgacgaag atttaaaagc gctgccggaa aaaggcggtt
     2461 atttgtgcct gtttgacgtc gataaattca aaaatattaa cgacaccttc ggtcatttgc
     2521 tgggcgatga agtgttgatg aaagtggtga aaatccttaa atcacagatc ccggtagata
     2581 aaggtaaagt ctaccgcttc ggcggtgacg aatttgcggt gatttatact ggcggcacac
     2641 tggaagagtt actatcgatt ctaaaagaaa tcgttcattt ccaggtggga agcattaatt
     2701 taagcaccag tatcggtgta gcgcattcaa atgaatgtac taccgtcgaa cgcttgaaaa
     2761 tgctggcgga tgagcggctg tataagagta agaaaaacgg cagggctcag attagctggc
     2821 agtagaatta gaatagtgtt agtacccctg aatcaagggg tactaatgaa tgtggtaaat
     2881 catttgcaaa cattttactt tgcaaaaact tccggccctt ttattgccaa tgtattcaaa
     2941 atacgtagtg ccgggccgct cggttttatt tcattacgtt cccatttcga gacactttct
     3001 tttgacattc ccattgtatg tgccagcacg gattgtgaca tgccgtatcg ggtgcggatg
     3061 gctttgatct cttcacccgt cattgatttg actttggcta tacgggcgct gacttcacgg
     3121 gctttgacca tgacggcaat ggcatcaacc tcactatcag acatcaggcc cagtgcattc
     3181 agttcctttg catcttccag catcatttgt aatttatcgc tcatttttca cctcaatcaa
     3241 gaagccagtt tcgatcaaat cctctatttg cttattgcta aaggccagaa atgcatttgc
     3301 catttttttg taggctgcca gttcatcatc ttcgatttct ttgcttcctt tactgcttag
     3361 accactcttg gaccaaccat cataaaaaaa cacatgattt gctgatttaa aaaagataat
     3421 tgttctggct ccaccactct ttccttgttg gaggggtaac cgttttttga tgacaccgct
     3481 acctaaatca gcctcccata tgccttgcat cacttcttca gcggcagcca tgagaacttt
     3541 atcgtgaata ccaagttttt tcgtgctttg gctgaatcgc cttgccttat acacacccat
     3601 acatccatcc ttgtggtaat gtttactata gtaaccatta cgcttatttc aagtaattgc
     3661 gatcaaaact ttaatttggt gtgaaaaagt gagagttgcc tcgagtacga ttcatttttc
     3721 tcgatttatg cgtcagggga ttttgtgcgt aagggtagga catcgggaag ggtagtagag
     3781 gcagatcgaa gaacaatatc cttgttagat ctgcctttag attaattaaa cccggctacc
     3841 ccacaaatcg tactcatcgg cgtgctcgac tttcacacgc aggatatcac ccggcttaac
     3901 gttggtttca ccattgagat aaaccgcacc gtcgatttcc ggtgcatctg ccatgctgcg
     3961 accaatcgcg ccttcttcgt ccacttcgtc gataatcacc agaatttcac ggcccacttt
     4021 ctcttgcagg cgctcggcgg aaatctgctg ctgcaactgc atgaaacggt tccagcgttc
     4081 ttctttcact tcttccggaa cctggtcagg cagggcattg gcgtctgcac cttcaaccgg
     4141 gctgtattta aagcagccaa cgcgatccag acgcgcttct ttcaggaagt cgagtagcat
     4201 ctggaaatct tcttccgtct cgccagggaa gccgacaata aaggttgagc gtagggtcag
     4261 ttccgggcag atttcgcgcc actgtttgat gcgcgccagt tggcgatcta cagaacccgg
     4321 acgcttcatc agtttgagaa tgcgcgggct ggcgtgctgc aacggaatgt ccagatacgg
     4381 caggattttg ccttctgcca tcagtgggat gacgtcgtcc acatgcggat aagggtaaac
     4441 gtagtgcaga cgtgtccaga tccccagttt cgataactgt tcgcacaggc tgaccatgct
     4501 ggtttttacc ggctcgccgt tgtggaagcc agtacgatgt ttaacatcaa cgccataggc
     4561 ggaagtatcc tgcgagatca ccagaatctc tttaacgccc gcatctacca gacgtttcgc
     4621 ttcacttaat acttcgccaa tcggacggct caccaggtcg ccgcgcatag acggaataat
     4681 gcagaaggtg cagcggtgat tacagccttc agaaattttc agataggcat aatgacgcgg
     4741 cgtcagtttg acaccttgtt ctggcaccag gctcaggaat gggttgtgtt tcggttttgg
     4801 cacgtagtga tgaacgtgct ccagaacctg ctcatagcta tgaggcccgg tgatttccag
     4861 cactttcggg tggacttcgc ggatctgatc ttcttttgcc cccagacaac cggtcacaat
     4921 taccttgccg ttttcattca acgcttcacc aatggcttcc agtgattctt gtaccgcgct
     4981 gtcaataaag ccgcaggtgt tgacgatcac catgtccgca tcgtcatagc tcggtaccac
     5041 gtcataacct tcagtgcgga gttcggtgag aatacgctct gaatcgacaa ggtttttcgg
     5101 acagccaagg gaaacaaagc cgattttcgg ctggggagtt actttgctca tagcttaaaa
     5161 aatattcagt tacaggaaag gtcagggcag ggattctaca gagttctgga taaaatttgt
     5221 atcgcaatct cattcgctgg cggaggcgaa ggaaatgtaa attttgttaa ttcggcgtga
     5281 agaattgatc ctggacagca ttatgctcaa aaaatagcca tactatttaa ttgcaacaag
     5341 gctggaaaga ggaggatcga agtatgttcg ttgacagaca gcgaatcgat ctgctgaacc
     5401 ggttgatcga cgcacgcgtt gacctcgccg catacgtgca actgaggaag gcaaaaggat
     5461 acatgtccgt cagcgaaagc aatcatctac gagataactt ttttaaactg aatcgcgaac
     5521 tgcacgataa atcgctgcgg ttgaatcttc atctggatca ggaagagtgg agtgctcttc
     5581 atcatgctga agaagcatta gcgacagccg cagtatgttt gatgagtggg caccatgatt
     5641 gcccgactgt tattaccgtc aacgccgata agcttgaaaa ttgtctgatg agcttaacgc
     5701 tgagtatcca gagcctgcag aagcacgcca tgcttgagaa ggcctgaaaa ctaaggggga
     5761 gaaagcgtct cccccttcat gtttaagttt ttgtaaaaat gaatttgtta tctcctccac
     5821 tgactacgct ttaagccaga gtcaatccgg aggcgttatg catcgacaat tctttttcct
     5881 tgtgcccctt atttgtcttt cttccgctct ctgggcggct cctgcaacgg taaatgtcga
     5941 agtactgcaa gacaaactcg accatccctg ggcactggcc tttttacccg ataaccacgg
     6001 tatgttaatc actctgcgcg gcggcgagtt gcgtcactgg caagcaggaa aaggattatc
     6061 tgcgccgctt tccggagttc cggacgtttg ggcgcacggg cagggcggcc tgctggacgt
     6121 ggttttagcg cctgattttg ctcagtctcg ccgcatctgg ttaagttatt ccgaagttgg
     6181 cgatgatggc aaagccggaa ctgctgtggg gtatggccgc ttaagtgatg atctctcaaa
     6241 agtgaccgac tttcgcaccg tctttcgcca gatgccaaaa ctgtctaccg gcaaccattt
     6301 tggcgggcgg ctggtattcg acggtaaagg ttatcttttt attgctctgg gcgaaaacaa
     6361 tcagcgcccg acggcgcagg atctggataa attacagggc aaactggtgc gtctgaccga
     6421 ccagggcgaa atcccggatg ataatccttt tataaaggaa tccggtgcgc gcgccgagat
     6481 ctggtcttat ggcattcgta atccgcaagg aatggcgatg aatccgtgga gtaatgcact
     6541 gtggctgaat gaacatggcc cgcgcggtgg tgatgaaatt aatatcccgc aaaaaggcaa
     6601 aaactacggc tggccgctgg caacctgggg aatcaactat tcaggcttta agataccgga
     6661 agcgaaaggg gagatcgtcg ccgggaccga gcaacctgtt ttttactgga aagattcgcc
     6721 cgccgtgagc ggcatggcct tctataacag cgataaattc ccccagtggc agcaaaaatt
     6781 atttattggc gcgctgaaag ataaagatgt cattgtgatg agcgtcaacg gcgacaaagt
     6841 aacagaagat ggccgtattt taacggacag agggcagcga attcgtgatg ttcgcactgg
     6901 acccgacggt tatttatacg ttctcaccga cgagtccagt ggggaattac ttaaagttag
     6961 cccacgcaat tagctaacgg gaatcatcac cactttgcgc actgcggggc gttcagtgag
     7021 ttgctgatac cagcgttgca gatttggacg cggtgtccag gtcaggccaa cattaaacaa
     7081 attgtagatg aacggtgcga tagcgatatc gcccacacca aactcgtcgc cagagaacca
     7141 ttttactttt gccagttccg catcgagcag ggcaaacagg gcgtcgcact ctttgcaact
     7201 ggcatcaatg gcggcctgat cgcgctcttc cggtggtgtt ctgactaatc ccatcaggat
     7261 cccgcgatga gcattactga gtgtctggtt tgcccagtcc atccattttt ctgcttccgc
     7321 acgacgtgcc ggtgagtcga tccacaggcg tttttgcccg tactgtgccg ccagatagcg
     7381 gacaatggcg tttgattccc aaagaatgag atcactttcg tcgtcacgca acaacggcac
     7441 cagcccgtta gggttcatcg ccagaaaatc agcatcgtga tttatcccaa actcacggcc
     7501 cgcgagaatt tgctcataag gtagttccag ttcttcgagc gtcagcaata ctttttttac
     7561 gttggttgaa ttattccgac cccacagcgt aatcatactc accccttttc cgacaggcag
     7621 ccacaggctg aacaggactc cgatggtgaa gtaaagttaa cttttaagca acagctggca
     7681 aaaaaatgac accagaagca gcgaagcggc aggatattgc ataaacttta aaaactttac
     7741 caacttacgg tttctttaag tttgtgtgtg cgttattaat caccgaactt atcatacggc
     7801 gatataacgt atttttttga atggatactc gggtggcatt tatgacgcaa tactcctctc
     7861 tccttcgtgg tcttgcagcg ggttctgcat ttttattcct ttttgcccca acggcattcg
     7921 cggccgaaca aaccgttgaa gcgccgagcg tggatgcgcg tgcatggatt ttaatggatt
     7981 acgccagcgg taaagtgctg gcagaaggca atgcggatga gaaactggat cccgcgagcc
     8041 tgactaaaat catgaccagc tatgtggttg ggcaggcgct taaggccgat aagattaaac
     8101 tcaccgatat ggtgacggtc ggtaaagatg cctgggcgac gggaaatccg gcactgcgtg
     8161 gttcatcggt aatgttcctc aaaccgggcg atcaggtttc ggtggcagac ttgaacaaag
     8221 gtgtgattat ccagtccggt aatgacgcct gtattgcgct ggccgattac gttgccggga
     8281 gccaggagtc atttattggt ttgatgaatg gttatgccaa aaaactgggg ctgaccaaca
     8341 ctaccttcca gacggtgcac ggtctggatg cgccggggca gttcagtacc gcgcgcgata
     8401 tggcattgct gggtaaagca ttgatccacg atgtgccgga agagtacgcc attcataaag
     8461 agaaagagtt caccttcaac aaaattcgtc agcctaaccg taaccgtctg ctgtggagca
     8521 gcaatctgaa tgttgatggc atgaagacag gaaccacggc aggcgcggga tataatctgg
     8581 ttgcttcggc tacccagggc gatatgcgtt taatctccgt agtgctgggg gcgaaaaccg
     8641 accgtatccg ttttaatgag tctgagaaat tattgacctg gggtttccgc ttctttgaaa
     8701 ccgtgacgcc aattaaacct gatgccacct ttgtgactca gcgcgtctgg tttggtgata
     8761 agagcgaagt gaatctcggg gcaggcgaag cgggctcagt gaccataccg cgtgggcagc
     8821 tgaaaaacct gaaggcgagc tacacgttaa cggaaccgca gcttaccgca ccgctgaaaa
     8881 aaggtcaggt tgtcgggacc attgatttcc agcttaacgg taaatccatt gagcagcgtc
     8941 cgctgatcgt gatggaaaat gtggaagagg gcggattctt tggtcggatg tgggatttcg
     9001 tgatgatgaa attccatcag tggttcggca gctggttctc ttaatcttct gataaccgga
     9061 tggcgcgaaa cgtcatccgg ttatacgtca ttaatacatc aacttaatac gctgcgtctg
     9121 cgcgtacttc acatactcat cttccgggca acaatcgctc accacaatat caaagcgttt
     9181 caggtcaccc atgcgcgccg gacgcacctt gccaaattta ctgtggtcaa caaccagcac
     9241 atgcttttgc gccatcgaca tggcccagtg ttttaccggc aactcttcaa gattaaaaca
     9301 ggtagcgcct ttactgacat gcacgcccgc cgcagaataa aaagcgatat ccgggcaaaa
     9361 attattcagc gtttgctgaa aatcgatggg tttgaaaatg gcgttgctgg cgtgaaattc
     9421 accaccgcac agaaatgcgc ggcaatgggg tttttctttc agcgccagaa aggtatttag
     9481 cgaataacaa acggcggtaa aagggatttc attatcaatc gcttcaataa tccacggcgt
     9541 ggtggtgcca cagtcaaaaa agagggtctg atcgggttct accagtgtcg cagccagttt
     9601 tgccgcccgg cgtttttctt ccaccaggcg ggatttttga tcgcttaaca ggtaatggct
     9661 ggcgctgcgc ggttccagaa caatatagcc gccgagcaaa acgacgggcg cactgtggtt
     9721 gttcagatcg cgacgaatcg tcatctccga aaccccaagc agggcggcgg cgtctttaag
     9781 atgtaactta tcgctgcgtt ttaattcttg cagcagctgc ccgatacgct cttcgcgacg
     9841 tgtttccata atccctctga atagttattg aagcgagccg ctcaatacta cactttttag
     9901 cagagatcag tcacgcaccc agcctttgcg gatcggtaat gcaaaacaga agcgatacca
     9961 cgattgcaga cgttgataga gtttatgccc catcgcttgc cagataatct gggcactaag
    10021 gcagccaatc atacctgcca gcaatccacc gagcatatcc agcggccagt ggacgccaag
    10081 ataaacgcgc gaccaggcaa tgacgacggc cagcaccatt aaaagtgagc cggaccacag
    10141 gcgatgccag cataaaaatg ccagtgcaaa ggtgaaaatc accgtaccgt gatcgctcgg
    10201 gaatgagtca tccgccgcat gatgcaggaa gttatagccg atattttcga caaacggtcg
    10261 gtcgtgcgga aaaagatgtc ccatcgtcca ggacaccaac aggctgaccg ccagcgcgat
    10321 agcgattttt atcaccagtt gccgttgtgc tgtaagcccc cacaaccaaa gtaccgcggc
    10381 cagcagcggc accacggtaa tcaaatcttt agcaataaaa atcgccaacg agatcatcca
    10441 cggagccgag tctggcgtcg cgttaataag agagaataga gagagattca aattttccag
    10501 cataacttcc cgacgcaaag tgattaaaag ggagccaata caggcaagtc gttgagaata
    10561 aagtgcaggt taaactgggt aaagcggcat cgtcttattt ccctcaagcg gcctgtttac
    10621 ggtgggtgat tgtaacgggc ataggttaaa taaaacttaa agaaagcgta gctatactcg
    10681 caataatgta agaatgtgct taaccgtggt ttcagctaca aaattcgctt tctcgttagc
    10741 tgcgctttta ttaaactctg cgcgattatt attggcgaag aaattgcatg caaaataaat
    10801 tagcttccgg tgccaggctt ggacgtcagg cgttactttt ccctctctgt ctggtgcttt
    10861 acgaattttc aacctatatc ggcaacgata tgattcaacc cggtatgttg gccgtggtgg
    10921 aacaatatca ggcgggcatt gattgggttc ctacttcgat gaccgcgtat ctggcgggcg
    10981 ggatgttttt acaatggctc ctggggccgt tgtcggatcg tattggtcgc cgtccggtga
    11041 tgctggcggg tgtggtgtgg tttattgtca cctgtctggc aatattactg gcgcaaaaca
    11101 ttgaacaatt caccctgttg cgcttcttgc agggcataag cctctgtttc attggcgctg
    11161 tgggatacgc cgcaattcag gaatccttcg aagaggcggt ttgtatcaag atcaccgcgc
    11221 tgatggcgaa cgtggcgctg attgctccgc tacttggtcc actggtgggc gcggcgtgga
    11281 tccatgtgct gccctgggaa gggatgtttg tcttgtttgc cgcattggca gcgatctcct
    11341 ttttcggtct gcaacgagcc atgcctgaaa ccgccacgcg tataggcgag aaactgtcgc
    11401 tgaaagaact cggtcgtgac tataagctgg tgctgaagaa cggtcgcttt gtggcggggg
    11461 cgctggcgct gggattcgtt agcctgccgt tgctggcgtg gatcgctcag tcgccgatta
    11521 tcatcattac cggcgagcag ttgagcagct atgaatatgg tttgctgcaa gtgcctattt
    11581 tcggggcgtt aattgcgggt aacttgctgt tagcgcgtct gacctcgcgc cgcaccgtac
    11641 gttcgctgat tattatgggc ggctggccga ttatgattgg tctgttggtc gctgctgcgg
    11701 caacggttat ctcatcgcac gcgtatttat ggatgaccgc cgggttaagt atttatgctt
    11761 tcggtattgg tctggcgaat gcgggactgg tgcgattaac cctgtttgcc agcgatatga
    11821 gtaaaggtac ggtttctgcg gcgatgggaa tgctgcaaat gctgatcttt accgtcggta
    11881 ttgaaatcag caaacatgcc tggctgaacg ggggcaacgg actgtttaat ctcttcaacc
    11941 ttgtcaacgg aattttgtgg ctgtcgctga tggttatctt tttaaaagat aaacagatgg
    12001 gaaattctca cgaagggtaa aaaaatgcct gattgctttg tgcgatcagg cattctcgaa
    12061 ttaatggtga tggtcgtcaa tctggtgttc gataaccatc ccttcaccta cgctggcaag
    12121 atggcgaaca taaggatgcg ggtggtaagc cggagctggc gcaggagcca catagacggt
    12181 ttgcggcaca gtcgcgacgc tgaccgcttg tggaacgctg actgaatcag gaaccacaac
    12241 cactttgtat ccactcggca catcgacggt gacactttgc gccatcgcca caccagtaaa
    12301 gcccattaaa agcgcgccca acagtagaca atttttcata atcatttcgc ctgaaataat
    12361 gcagcgggtt aatttccgcg aattatgcag ccttcattca ggtattgtgt tgccgggcgt
    12421 gctgccgttt ttattggtca aatggcgctt cgtgtttaag aactttatcg atcacatcca
    12481 gtacgccttc acggttattg gagcctgccc ggtattttgc cgctgcgacg accgcgttgc
    12541 cggcattttc cattgcaaaa ctaaagcctg cctgacgcag catctcaata tcgttaccgc
    12601 catcgccaaa gaccaccact tcgctgtcgt ctattcccca taatttctgc agttggcgaa
    12661 ggccattggc tttatgtacg ccggggataa tcagatcgat gctgccgttg ccggtgtgga
    12721 ccggcaccat aatatcgccg atggcctcat gtaatgtttt ttgtacttgt ggaatcagtt
    12781 catcggaaag attcaggcca aacttaaaga agatatcttc taagttgtca aagttatcga
    12841 cgtattccag acggtgataa tacatttccg ccaccatttt catggcatcg tcatattttt
    12901 tgagcgtata ggcgctattt tttccgcagg caataatttc cacttccggg cgcgtcagca
    12961 aatgttccac gacagtagta aacgcatcct tcgacagctc gccattaaaa acatctttgc
    13021 cttcactcac tacccagccg ccgttttcag ccacaaaggc aatttcatta gcaatttcag
    13081 ggaagaaaga gatcaactga taatattgat tcccgctggc gaccacaaag cgaatgcctt
    13141 gtgctttcat ttgctgatac tgagccataa accgctcacg gttataggtt ttttgatcgc
    13201 ttaagaaagt accatccatg tctaccgcaa ttaatttaat gctcatcaac tattctccat
    13261 cgccgtctgc gttttggtat cgggtttggc gacggctttc gcgacaatag ccgcgagaat
    13321 aaccagcgcc agtacaacca gcattgcact acgtaatcca taatgttcgc cgagatagcc
    13381 cagcagcggc ggcccgacga ggaaagccag ataaccggtc gttgctacca cactgacgcg
    13441 ggttggtgca tcggggccgg tatcgctggc ggcagaaatg gtcagcggga agcccagcga
    13501 ggcacccagt ccccagagta caacagacac cccagcgacc caggcgctat cgacaaaaat
    13561 aatcagccca atacccaacg cccccattag cgcactggcc cgaaccacgg caacgcgact
    13621 gtaacggtcg atgaaccaac cgccagtgaa gcgtccaacg gtcatcccca gggtaaaacc
    13681 ggcataaatc agcgagccgg aagtggggct aaaaccgtga ccgtcaacca ttaataaggg
    13741 taaccagtcg ttggcagaac cttcggcaaa ggccatcgcc agcaccacaa caccaatcag
    13801 cagcaactgg atatcgcgat aaaaaggtac gcctttttcg ccatgctggg tgccatcggc
    13861 agcatttttg cccgtaccgt cagggattgc ctgaatggcg atatatatag gcgcgatgcc
    13921 taccagcgcc gccaataaaa tgtgcaccgt tgccggaacg ccaaaggccg tcagtgccat
    13981 cccgacacca gcgcctgcca gcgtgcccag gctataaaaa ccgtgcatca tcggcaaaac
    14041 cgttttattc atttctcgct caacggcggc accttcaacg tttatcgcca cttccgcaga
    14101 accaaaactt gccccaaaga cgccgagacc aacggcaaag agcaggggcg atgtcagcca
    14161 gagtgccaga cttaatatca tcatcccgac caatgcgcag gacatggtga ccaggatgac
    14221 attacgtgtc ccaaagcgtt tcactaacca cgccgagcag agaataccgc tcatcgaacc
    14281 gatcgacaga ccaaagagaa caccgcccat ttcagcgatc gagacagaga gaatatcgcg
    14341 gatagcaggc gtacgggttg cccaggacgc cattaacagg cccggcaaaa agaagaacat
    14401 aaacagcgcc caggttcggc gtttcaatgc attacgtgaa gaatttacgg tcatagatca
    14461 cgtcaaaata agaagaggga agacaacatt agcaaggttg tgtacatttg tacacaattg
    14521 cagacagagg aaatgacatg cgtcgcgcta acgatccgca acggcgagaa aaaattatcc
    14581 aggccacgct ggaggcggtg aaactttacg gaatacatgc tgttacgcac cgcaaaattg
    14641 ctacccttgc cggggtaccg ttgggatcga tgacctacta tttttcagga attgatgagt
    14701 tgttgctgga ggcgttcagc agttttactg agatcatgtc ccggcaatat caggcatttt
    14761 ttagcgatgt tagtgatgct caaggcgcat gccaggctat caccgatatg atctacagct
    14821 cacaggttgc aacgccggat aacatggagc tgatgtacca gctctacgcg ctggctagcc
    14881 gaaaaccgct attaaaaacg gtaatgcaaa actggatgca gcgcagtcag caaacgctcg
    14941 aacaatggtt tgaacccgga accgcccgcg cgcttgatgc gtttattgag gggatgacgc
    15001 tgcattttgt caccgaccgt aagccgctat cgcgcgagga gattttgagg atggttgaga
    15061 gggttgcagg gtagtagata aatttcaggc aacaaaaaac ccatcaacct tgaaccgaaa
    15121 tggcggggtt gatgggctcc acaaaatggg gacatcaaag aaaagcagtg gcactaatta
    15181 agactgatgc cctgcggaaa agttctgcgg ttgtgcaaaa aaatttcatt ttcagggcaa
    15241 cttcagtttt atcctaatcc tggccatacc atgacgatga ttgtccctgc cagcgtcagc
    15301 aggacgttgg cgattgcgta ggtgcccgca tagcccagcg ccgggatgtt actgcgagct
    15361 gtatcactga tgatctccat tgccggcgcg caggtgcgtg cgcccatcat tgcgccgaac
    15421 aacagtgcgc ggttcattcg caatacataa gcaccgaaca agaaacagat aaccacgggc
    15481 acaagactga caatcaatcc ggcaatcaac atctgaccgc caatcgcgcc cagaccgtta
    15541 ttaataccgc taccggcgct cagaccaacg cctgccataa acaccatcaa gccgaactct
    15601 ttcaccatgc ttaatgcccc ttgcggaatg taaccgaagg tcgggtggtt agcacgcata
    15661 aagcccagca taattccggc gaataacaac ccggcagcgt tccccatgcc gaaactgaat
    15721 gtgctgaact ggaaggtgat catcccgatc atcagcccaa taacaaagaa ggcgcagaat
    15781 gccagcaggt cagtgacctg gctgtgaatc gagataaagc cgatgcgatc ggcgatggtt
    15841 tttacgcggc gggcatcgcc gctgacttgt aaaacgtcac ctttgttaag cacgacgttg
    15901 tcatcaatcg gcatctcaat ctggctacga atgacgcggt taaggaagca accgtgatcg
    15961 gtcaacttca gttgtgcgag acgtttacct acagcgttat ggtttttaac gaccacttct
    16021 tcagtgacga tacgcatgtc gagaaggtca cgatcgaaaa cttctttacc gttacggaag
    16081 ctgggatcga gtcgggcatg ggcgtcggga tagcctacca acgctatttc atcgcccatt
    16141 tgtagcacgg catcaccgtc tggatttgcc agaatcccgt tacgtcgaat acgttcaatg
    16201 tagcagccgg tttgtcgata aatacccagt tcacgcagat ttttgccgtc ggtccaggcc
    16261 accagctccg ggccgacgcg ataggcgcgg atcaccggta aataaacctt acggttggca
    16321 tcagtgtcca ggccacgttc gcgggcgatt tgctgggcgc tggtctgtaa gtcctgatgc
    16381 tgcaatttcg gcaagtaacg cgcaccaaca atcaaactca ccagaccgat taaataggtt
    16441 aaggcatacc cgaggctcag attatccagt gccagtgaga gctgcctgct ttccataccg
    16501 gaatgacgca gtgtatcgcc agcaccgacc agaaccggtg tcgacgtcat agagcctgct
    16561 aacataccgg ccgtcaggcc aatatcccag ccaaacagct tacctaaccc taaggcgatc
    16621 accagcgcac tgccaaccat caccagtgct aacattaggt aatttttccc atcgcgaaaa
    16681 aaaatggaaa aaaagttcgg tccggcttcg accccgacgc agaaaataaa cagcataaag
    16741 ccaagattaa gcgcatcggt gttaatgctg aaatgttgtt ggcctaataa cagcgatacg
    16801 actaaaacgc caatggaatt acccagttgg atcgaaccaa gtcgtaactt tccgagacat
    16861 agcccaagcg cgaggaccac aaataataac agaatgtaat tcccatttaa caattcggcg
    16921 acgtttatat tcacggaggc taacttcttg tttactagta aactgttgaa agaaatggta
    16981 atttacgata atgtttttta ccagaattca gggcgcagat tcattcagcg cacctaaacg
    17041 atagtaaagt aacaatatat tttactagtg taatcacatt aggtatcaac ggctatatga
    17101 attgcgttgg cctatattag catggaatgc gaagcggctt tatcttactg aacgccacac
    17161 tggcgaaaaa tgtgttcgat agacgcagtg tcaggaggaa cgagtgaaac ataaacaacg
    17221 ttgggcgggg gcaatctgct gttttgtcct cttcattgtg gtgtgccttt ttctggcgac
    17281 gcacatgaaa ggcgcttttc gggctgccgg gcatcctgaa atcggcttgc tgtttttcat
    17341 tcttcctgga gcagttgcca gtttcttttc acagcgtaga gaagtcctga aacctctatt
    17401 tggcgcaatg ctggcggcac cctgttcaat gcttattatg cggctgtttt tttcaccgac
    17461 ccgctcattc tggcaagagc tggcatggtt actaagcgcg gtgttctggt gtgcgctggg
    17521 ggcactgtgt ttcttattta tcagtagctt gtttaaacca cagcacagaa aaaatcagta
    17581 aagccctcaa cgcgagggct tgtcagacga tcaggcgtcc agattctctt tcacccatgc
    17641 agcaaaatcg gtatagccgc cgatatgttg ctgatcgaca aaaatctgcg gcacggtttc
    17701 tacgggttta cctgcctttt gttgtagatc ttctttagtg atcccttccg cacgaatatc
    17761 tacatactga tactgaaaat catcgcgttc attgctcaat ttctcagcca gatcttttgc
    17821 acgcacacag taagggcaac ccggacgacc aaaaataacg gtttgcatta tttctctcct
    17881 catagattta tgcctgtaat gatcacgcta aaatgtattc gctgaaagta ggtttaacct
    17941 gttgcattaa ttgctaaaag ctataactgt taaacacaat acagtgaaaa gttttagact
    18001 gaaggttcac tttgcagagg gaagcgtatg cgcgcgatcg gtaaattgcc taaaagcgtg
    18061 ttgatactgg aatttatcgg aatgatgttg ctggcggtgg cgctgctgtc ggtaagcgac
    18121 tccctgtcgc tgcctgagcc attttctcgg ccagaagtgc agattctgat gatttttctc
    18181 ggtgttttgc tcatgcttcc cgctgcggtg gtggttattc ttcaggtggc aaaacgtctt
    18241 gccccacagc tgatgaaccg tccaccgcag tattcacgtt cagaaagaga aaaagataat
    18301 gacgccaacc attgaactta cttgtggcca tcgctccatt cgccatttca ctgatgaacc
    18361 catttccgag gcgcagcgtg aggcgattat taacagcgcc cgtgcgacgt ccagttccag
    18421 ttttttgcag tgcagtagca ttattcgcat taccgacaaa gcgttacgtg aagaactggt
    18481 gacgctgacc ggcgggcaaa aacacgtagc gcaagcggcg gagttctggg tgttctgtgc
    18541 cgactttaac cgccatttac agatctgtcc ggatgctcag cttggcctgg cggaacaatt
    18601 gctgctcggt gtcgttgata cggcaatgat ggcgcagaat gcattaaccg cagcggaatc
    18661 gctgggattg ggcggggtat atatcggcgg cctgcgcaat aatattgaag cggtgacgaa
    18721 actgctgaaa ttaccgcagc atgttctgcc gctgtttggg ctgtgccttg gctggcctgc
    18781 ggataatccg gatcttaagc cgcgtttacc gtcctccatt ttggtgcatg aaaacagcta
    18841 tcaaccgctg gataaagacg cactggcgca gtatgacgaa caactggctg aatattacct
    18901 cacccgtggc agcaataatc gccgggatac ctggagcgat catatccgcc gaacaatcat
    18961 taaagaaagc cgcccattta ttctggatta tttgcacaaa cagggttggg cgacgcgcta
    19021 aaaccgccac gccgatgtat gatacgcggg cttttgacca ggtctgacag agaggtgcag
    19081 ggtgaaaatt gccatattgt cccgggatgg aacgctctat tcgtgtaagc ggctgcgtga
    19141 agccgctata cagcgcggtc acctggttga aattcttgat ccgctttctt gctacatgaa
    19201 cataaatcct gcggcgtctt ctattcacta caaaggccgc aagttacccc attttgacgc
    19261 agtgatcccg cgtattggca ccgccattac cttttatggg acggcggcac tgcgccagtt
    19321 cgagatgctg gggagctatc cgctcaatga gtcggtcgcc attgcccggg cgcgtgacaa
    19381 attgcgttcc atgcaactgc tggcgcgtca gggcatcgac ctgcctgtca cgggcattgc
    19441 gcattcgccg gatgatacca gcgatttaat cgacatggtc ggtggtgcgc cgctggtggt
    19501 caagttggtt gaaggcacgc agggaattgg cgtcgtgctg gcggagacgc gtcaggcggc
    19561 ggaaagcgtg attgacgctt tccgcggtct gaacgcgcat attctggtgc aggaatatat
    19621 caaagaggcg caagggtgcg atatccgctg tctggttgtt ggcgatgaag tggtcgctgc
    19681 gattgaacgg cgggcgaaag agggcgattt tcgttccaat ttgcatcgtg gcggcgcggc
    19741 aagcgtcgcc agtatcacac cacaggagcg tgaaatcgcg ataaaagccg cgcgaacgat
    19801 ggcgctggac gttgctggtg tggatattct gcgtgctaat cgcgggccgt tggtgatgga
    19861 agtgaatgcg tcgccggggc tggaaggaat agaaaaaacc accggtatcg acatcgcggg
    19921 taaaatgatc cgctggatcg aacgccacgc tacgacagaa tattgcctga aaacgggtgg
    19981 ttagtcgcaa tcacattact gatcatggtt ttgcctgctc tttttgcgta agctgtgccg
    20041 gtctttttat cgaaagaggt tgtacaaaat tatgacatcg ctggtcgttc ctggtctgga
    20101 tacgctgcgt caatggctcg atgacctggg gatgagtttt tttgaatgtg ataactgtca
    20161 ggctctgcat ctgccccata tgcagaattt cgacggtgtc tttgatgcca aaatcgatct
    20221 gatcgataac acgatcctgt tttctgccat ggcggaagtc cgaccttcag ccgtattgcc
    20281 gctggcggcg gatttatctg ccatcaatgc cagttcgctg accgtgaaag catttcttga
    20341 tatgcaggat gataatctgc caaagctggt ggtttgccag tctttatccg ttatgcaggg
    20401 cgtaacctat gagcagtttg catggttcgt gcgtcagagc gaagagcaga tttcgatggt
    20461 cattcttgaa gctaatgccc atcaactgct gttaccgact gacgatgaag ggcaaaacaa
    20521 cgttaccgaa aactatttcc tccactgata actcctttcg agcacgcagt cgctggtgca
    20581 gtggctgcgc gctgcaaaat tatctgctgt ttttaacctt ttcttaaaga ttatttcact
    20641 tctcttgtgt cgatttggat ttatcacata gagcaaatac gcataaaaat ttgttaaata
    20701 ccgtttttta atccgagcta tagtctcaaa ccctggctaa agttattctt gcgatgcttt
    20761 tatatagcga gcagtgctgg ccgggagaga gttctctttt cttacaccgc gccgataaaa
    20821 aatatgcacg tttattgcat atctttcagt gtgacaactt ttgttcgttt gttaacgaac
    20881 tttcagaagg aaagagatat gaccgcctta aataaaaaat ggctttcggg tctggttgcg
    20941 ggtgctctga tggccgtctc tgtcggcacg ctcgcggctg aacaaaaaac actccacatt
    21001 tataactggt ctgattatat cgcccccgac acggtggcta actttgaaaa agaaaccggt
    21061 attaaagtcg tctacgatgt tttcgactct aacgaagtac tggaaggcaa attaatggcc
    21121 gggagcaccg gctttgacct ggtggtgccg tcagcaagct ttcttgaacg ccagctgaca
    21181 gcgggagttt tccagccgct ggataaaagc aaattgccgg agtggaagaa tctcgacccg
    21241 gaactgctga agttggtcgc caaacacgat cccgacaata aatttgctat gccctatatg
    21301 tgggcgacga ccgggattgg ctataacgtc gataaagtca aagcggtgct gggcgaaaac
    21361 gcgcccgtcg atagctggga cttgatcctc aagcctgaaa atctggaaaa actgaaaagc
    21421 tgcggtgtat ctttcctcga tgcgccagaa gaagtttttg ctaccgtgct gaattatctc
    21481 ggcaaagatc ccaacagcac taaagcggat gattacaccg gaccggctac cgatctgctg
    21541 ttaaagctgc gcccgaacat tcgttatttc cattcatctc aatatattaa cgacctggca
    21601 aacggcgata tttgcgtcgc tatcggctgg gcaggtgatg tctggcaggc gtcaaaccgc
    21661 gcgaaggaag cgaagaatgg cgtgaatgtc tcgttctcga ttccaaaaga aggggcgatg
    21721 gcgttctttg atgtattcgc catgcctgcg gatgccaaaa ataaagacga agcctatcag
    21781 ttcctgaatt acctgctgcg tcctgatgtg gtggcacata tctctgacca tgtgttctat
    21841 gctaacgcca ataaagcagc cacgccgctg gtgagtgcgg aagtccgtga taacccgggt
    21901 atttatccgc ctgcggatgt ccgcgcgaag ttgttcactc tgaaagtaca ggctccgaaa
    21961 atcgaccgtg tgcgcacccg cgcgtggacc aaagtgaaga gcggaaaata atccgcagcc
    22021 ttagatgccg ggcgggagcg ccactccctc cggcaattcg caccaacatg gtgcgcttgc
    22081 acacatttaa tgccggagag cagccgtgaa tgacgctatc cctcgccccc aggcgaaaac
    22141 ccgtaaggcg ctgaccccgc tattagaaat ccgcaatctg accaaatcct acgatggtca
    22201 gcatgcggtt gatgatgtca gcctgaccat ctacaaaggt gaaatctttg cgctgctggg
    22261 cgcatccggc tgtggcaagt ccacgctgct gcgtatgctg gcaggtttcg aacaaccttc
    22321 agccgggcag attatgcttg atggcgtcga tttgtcacag gtgccgcctt acctgcgccc
    22381 catcaatatg atgtttcagt cttacgcgct gtttccgcat atgaccgtgg aacagaacat
    22441 cgcttttggc ctgaaacagg acaaactacc gaaagcggaa attgccagcc gggtcaatga
    22501 gatgctcggc cttgtgcata tgcaggagtt cgccaaacgc aaaccgcatc agctttccgg
    22561 tggtcagcga cagcgtgtgg cactggcccg aagccttgcc aagcgcccga aactattact
    22621 gctcgacgag ccgatgggcg cgctggataa aaagctgcgt gaccggatgc agcttgaagt
    22681 ggtggatatt ctggaacgcg tcggtgtgac ttgtgtgatg gtcacccacg atcaggaaga
    22741 ggcgatgacc atggcggggc gtatcgccat tatgaatcgt gggaaatttg tccagattgg
    22801 cgaaccggaa gagatctacg agcatccgac tacccgctat agcgccgaat ttattggctc
    22861 ggttaatgtc tttgaaggtg tactcaaaga gcgtcaggaa gatggtctgg tgctttattc
    22921 gccggggctg gtgcatccac tgaaagtcga tgcggatgct tcagtggtcg ataacgtgcc
    22981 ggtacacgta gcgctgcgtc cggaaaaaat catgctttgc gaagagccgc ccgccaatgg
    23041 ttgtaacttc gcggtggggg aggtgataca cattgcctat ctcggcgatc tttcggtgta
    23101 tcacgttcgt ctgaaaagtg ggcagatgat tagcgcccag ctacaaaacg cccatcgtca
    23161 tcgtaaaggg ttaccgacct ggggcgacga agtgcgtttg tgctgggaag tggacagctg
    23221 tgtggtgctg acggtttaag gagcaaagat gagtacactt gaacctgctg cccagtcgaa
    23281 accgccgggc ggatttaagc tgtggttgtc gcagttgcaa atgaagcatg ggcgcaaact
    23341 ggtcattgcg ttgccgtata tctggttgat cttgctgttt ctgctgccat ttctgattgt
    23401 ctttaaaata agcctggcgg agatggcgcg cgctattcca ccttataccg aactgatgga
    23461 gtgggctgac gggcaactgt ccatcaccct taatttcggt aatttcctgc aactgaccgg
    23521 cgatccgctc tatttcgatg cttatctcca gtcgttacag gtggcggcga tttcgacatt
    23581 ttgctgttta ctgatcggct atccgctggc gtgggcggtg gcgcacagta agccttcgac
    23641 ccgtaatatc ttattactac tggtgatcct gccgtcgtgg acctcgtttc tgattcgtgt
    23701 ctacgcctgg atgggaatat taaaaaacaa cggtgtgctg aataattttc tgctgtggct
    23761 gggggttatc gatcaaccgc tgaccattct tcataccaat ctggcagttt atattggcat
    23821 tgtttacgcc tacgtgccgt ttatggtgct gccgatttat accgcgttga ttcgtattga
    23881 ttattcgctg gtggaagcag cgctggatct cggtgcacga ccgctgaaaa cgttctttac
    23941 tgtgatcgtg ccgctgacta aaggtgggat tattgccgga tcgatgctgg tgtttatccc
    24001 ggctgtgggc gagtttgtga tcccggaact gctcggtggc ccggacagca tcatgatcgg
    24061 gcgcgtgcta tggcaagagt ttttcaataa ccgcgactgg ccggtggcct cggcggtagc
    24121 gatcatcatg ttgctgctgc taattgtgcc gataatgtgg tttcacaaac accagcaaaa
    24181 aagcgtggga gaacacggat gaataattta ccggtagttc gttcgccctg gcggattgtg
    24241 attttgctgt tgggcttcac ctttctctac gcgccaatgc tgatgctggt tatctattcg
    24301 tttaacagct cgaagctggt gacggtgtgg gccggctggt caacgcgctg gtatggcgaa
    24361 ttattgcgcg atgacgcgat gatgagtgcg gttggtttaa gtctgacaat tgcggcctgt
    24421 gcggcgacgg cggcggcgat cctcggaact attgcggcgg tggtgctggt gcgctttggc
    24481 aggtttcgcg gatcaaatgg ctttgccttt atgatcaccg cgccgctggt aatgccagat
    24541 gtcatcacgg gcttgtcgct gttgttatta ttcgtcgcgc ttgctcatgc cattggctgg
    24601 cctgcggacc gcggtatgct taccatctgg ctggcgcatg tcactttctg tacggcttat
    24661 gtggcagtcg ttatttcgtc gcgtcttcgg gaactggatc gctcgataga agaagcagcg
    24721 atggatctcg gtgcgacgcc gctgaaagtg tttttcgtca ttacgctacc gatgatcatg
    24781 cccgcggtca tttctggctg gttactggct tttactttgt cgcttgatga tctggtgatc
    24841 gccagctttg tatccgggcc gggagccacc acgttaccga tgctggtctt ttccagcgtg
    24901 cggatggggg tgaatccgga aatcaacgcc ctagcaacat tgatcctcgg cgcggtcgga
    24961 attgtcggat ttatcgcctg gtatctgatg gctcgcgcag aaaaacagcg gatacgcgac
    25021 atccagtgtg caagacgtgg ctgaagacac taaaatttgc caacctggct acataatgcc
    25081 gcgtatgtcg cggcattgtt ttcatggaag acgaaacgtt gggatttttt aagaaaacat
    25141 cttcatctca tgctcgcctg aatgtgcctg cgcttgtgca ggtggcggcg ctcgccatta
    25201 ttatgatccg tggcctcgac gtgctgatga ttttcaatac gctgggcgtg cgcggtattg
    25261 gcgagttcat tcatcgcagc gtacaaacct ggagtttaac gctggtcttt ttaagcagtc
    25321 tggtgctggt tttcattgag atctggtgtg cgttttcact ggtgaaaggg cgtcgctggg
    25381 cgcgctggct gtatctgctg acacaaatca ctgccgcaag ttacttgtgg gcggcttcgc
    25441 tggggtacgg ttatccggag ctgttcagca ttcccggtga atcaaaacgt gaaatcttcc
    25501 atagcctgat gctgcagaag ctgccggata tgctcatcct gatgctgctg ttcgttccct
    25561 cgaccagtcg gcggttcttc cagttgcaat aatgtgtata atcgtcgccc ctgatgatgt
    25621 gaaggtcaat gtatgcagtg cgcactttac gacgcgggtc gctgtcgttc ctgtcagtgg
    25681 ataacgcagc cgattccaga gcaactctcc gctaaaaccg ccgatcttaa aaatctgctc
    25741 gccgactttc cggttgagga atggtgcgcg ccggtgtcag gcccggaaca agggtttcgt
    25801 aataaagcca aaatggtggt gagtggtagc gttgaaaaac cactgctcgg tatgctgcat
    25861 cgcgacggca caccggaaga cctttgtgac tgcccgcttt atcctgcctc atttgcgccc
    25921 gtttttgcgg cgctaaaacc gtttatcgcc cgagcggggt taacgcccta caacgtggcg
    25981 cgtaagcgtg gcgaactgaa atacattctg ctgactgaaa gccagagcga tggcggcatg
    26041 atgctgcgct ttgtactgcg ttctgaaacc aaactagcac aactgcgtaa ggcattgccg
    26101 tggttacagg cacaattgcc gcagctaaaa gttatcaccg tcaatattca gccggtacat
    26161 atggcgatta tggaagggga gacggagatc tacctgaccg aacaacaggc gctggtagag
    26221 cgttttaacg atgtgccgct gtggatccgt ccgcaaagtt tcttccagac taatccggcg
    26281 gtcgccagcc agctgtacgc taccgcgcgc gactgggtac gacagctgcc ggttaaacat
    26341 atgtgggatc tgttctgcgg tgtggggggc tttggtttac actgcgcgac gcctgacatg
    26401 cagttaaccg ggatcgaaat tgcgctagag gccattgcct gtgcaaagca gtcagccgct
    26461 gaactgggct taacgcgtct gcaatttcag gcgctggact ccactcagtt tgccaccgct
    26521 cagggggagg tgccggagct ggtgctggtt aacccgccgc gccgcggcat tggtaaaccg
    26581 ctgtgtgatt atctctcaac gatggcaccg cgttttatta tctactccag ctgtaacgcc
    26641 caaaccatgg cgaaagatat ccgcgaactg ccaggttacc gcatcgaacg ggtacagctt
    26701 tttgatatgt tcccgcacac cgcgcactat gaagtgctga cgctgctggt aaaaatgtaa
    26761 atacatcatt caaatcaata tcacctcggc atacatccat ccaaaataat aatcgtgatc
    26821 gatcgattaa taataaccga tcgatcacgg ttatatattg ctctattttt gcaaatcaaa
    26881 tcacaaataa tcaaataaga tctttaattg aactgttttg attactacag tgcacttacc
    26941 gaatacatac agaggcattg cttagcgaaa cacaaaattt cctgagacat tattaacgtc
    27001 agcagaaggt tcatgcattg acaacggaaa tgtacgcgta tggagggcgt tccgacgatg
    27061 tttgccaaat tcatagatgt tattcaaacg tttttaactg aaccggcaat tttaattggt
    27121 ttgctggtgg gtattggtta tgcccttgat aagaaatcgc caataaaaat tattaccggc
    27181 atggtcagcg ccatggtggg attaatgatg gtgttatttg gtggtttcca gttttccgca
    27241 acatttaaac cggtagcaga agccgtaagt aaagcctatg gtgttcacgg atatttaatg
    27301 gattcctacg caatgaaagc ggcaacgcaa attgcattgg gggataattt tggttatgtc
    27361 gggtatgtat ttgtactggc gttttttacc aacctgctgc tggtattatt tggtcggtat
    27421 actggtgcca aagggatatt tttgacaggg aataccggtg tttcacattc tcaggcggta
    27481 ttatggttga ttgtcttttg gctgggtttt agctggacta catccattat tattgccgga
    27541 atattaacgg gtgtattctg ggcgttctcc accacgctca ttgttaagcc cattgccaaa
    27601 gttaccaaag atgccggttt taccattgct cataaccaga tgttgggatt gtggtttttc
    27661 tccaaattcg cccataaatt tggtgaccct gaaaagcacg atgctgaaaa cctgaaactt
    27721 cccgggtggc tggcgatttt taaccataac gtaacggcta tcgccattgt gatgacgctg
    27781 tttgtcggtg gtttcttgct atcaacgggt atcgataatg tccagttaat ggcgaaaggc
    27841 aagccctggt acatctatat catcaacctt ggcttacagt tctccatgta tatggtcatt
    27901 ctgctgcagg gtgtgcgcat gatggtgggc gaaattaacg gttcgtttaa aggctggcaa
    27961 gatcgcttta ttcctaacgc tattcctgct gtcgatgttg cagcgctgtt acctttttca
    28021 cccaatgccg caacgttagg cttcgtcttc tgtacttttg gcaccatttt ttcgatgggg
    28081 atcttattac tggttcacag cccaattatg gtattgcctg gctttgtacc tctgtttttc
    28141 tctggcggtc caattggcgt attagcaaac cgcatgggcg gataccgttc cgtaattatc
    28201 tgtacgttct tgctgggtat tattcagacc ttcggtacgg tgtgggctat tccgttaacc
    28261 gggcttgctg aaaatggcgt cggctggaca ggaatatttg actgggcaac cgtatggcct
    28321 gctatttgtg aagttctgaa atttatcgct gcaacattcc atcttggtcc ttacgcgggt
    28381 taattaaata ttaagggttt aaaaatgaaa gatacaatta atattctgtt tgtctgtggt
    28441 tacggtgtag gcagtagcgt tatgttacaa acggtcgtta aaaaagcgct gaccaaatat
    28501 gatttcaaag ttgaaatgga acataccgcc gcaggtgaag tcggcggatt taccgactgg
    28561 gcggatattt ttgctatttc caaaaaatta ctcgatgtgg ttagtcttga cccgaaacaa
    28621 gggcaatatt tgattccgat agagaatatt atggacggtg aaactattgg tcagcaaatt
    28681 tatgaagtgg ttcagcaaca ttttccgcat ttaattaatc aataaggtag ggcgtaatga
    28741 gagcgattat tctgctgttc gatagtctga ataaacgtta tctgccacct tatggcgatg
    28801 cgttaaccaa agcgcctaat ttccaacgtc tggcggctca tgccgccacc tttgaaaaca
    28861 gttacgtcgg cagtatgccg tgtatgcctg cccggcggga gttgcatacc gggcgatgta
    28921 acttcctgca tcgggagtgg gggccgttag aaccgtttga cgattccatg ccggagctgc
    28981 ttaaaaaggc ggggatctat acccacctga taagcgatca tttgcattac tgggaagatg
    29041 gcggcgagaa ttatcataat cgctacagtt cgtgggagat tgtgcgcgga caggaaggcg
    29101 atcactggca tgcaagcgtt gcgcaaccgc ctattcccga ggtgctacgg gtgccgcaaa
    29161 agcagaccgg tggcggtgtt tctggtctgt ggcgccatga ctgggcaaac cgggaatata
    29221 ttcagcagga agcggatttt ccacaaacta aagtgtttga tgcaggctgt gcctttatcc
    29281 acaaaaatca cgcggaagat aactggttat tgcagatcga aacgtttgat ccgcacgagc
    29341 cgtttcacac aacggaagag tatctttcct tgtatgaaga taactgggat ggaccgcatt
    29401 atgactggcc gcgtggccgg gtgcaggaaa gcgacgaggc cgtggagcac atccgttgcc
    29461 ggtatcgttc gctggtgtcg atgtgcgatc gcaacctggg gcgcattctc gacctgatgg
    29521 atgagcacga tctgtggcga gataccatgc tgattgtcgg cactgaccac ggctttttac
    29581 tcggtgagca tggctggtgg gcgaaaaacc agatgcctta ttacaacgaa gttgccaata
    29641 atccgttgtt tatctggaac ccgcgcagtg gtgtaaaagg agagcggcga caggcactgg
    29701 tacaaatgat cgactgggcg ccaacgttgt atgacttttt tcaacagcca gtgccgcccg
    29761 atgtgcaggg gcaaccgctg gcgaaaacgg tcagtcacga tgaaccagta cgcagctcgg
    29821 cgatgtttgg tgttttcagt ggtcatgcta acgtaactga tgggcgttat gtgtatatgc
    29881 gtgcagcgct gccggggcgt gaggatgata ttgccaacta cacgttgatg tcctgcaaaa
    29941 tgaacagccg ctatccggtg gatgagatgc gggctttatc gctggcccca ccgtttcgtt
    30001 ttaccaaagg gttacaggta ttacgcatcc cggcacagga aaaatataag gggttgaatc
    30061 agtttggtca tttgctgttt gatctgcaaa acgatccgca gcagctacat ccgattcatg
    30121 atgatgtgat cgagtcccgg atgatcgcgt tgctgattca gttgatgaaa gataatgatg
    30181 cgccgccaga gcagtttcag cgcctgggat tagcgtagag aagtgatttt gccggagtgg
    30241 gtgttcgccc actccggcaa atgaataatt attgcgggaa ccactgatcg ctgatttgtt
    30301 gatatgtacc gtcagcttta attgctgcca gcgcgttatt cagtttttcc agcagagctt
    30361 tgttatccgg acgtacagcg atacccagac cggtgccaaa gtattgcgga tcggtcactt
    30421 tttcagtagc aacgcccagt tgcggattgg ttttcagcca ttcattaacc accgcagtgt
    30481 caccaaatac gccatcaata cggctatttt tcagatcgat aaaggcattt tgataactgt
    30541 cataggagac cgttttcact tccgggtgct gatcctgaat atatttctgg tgcgtagtac
    30601 cgttttccat cccaatgcgt ttgcctttca ggtcggcaaa cgttttgtag gtatcttttt
    30661 tggcaatcac gacggctgag ttttcgtaat agggcgtggt gaacgatacc tgcttgctac
    30721 gatctggtgt gatatccata ccggagatta cggcgtcata tttcctgaat ttcagtgacg
    30781 ggatcaggct gtcgaacgcg tgattggtaa aagtacattc tgcctgcatc tgtttgcata
    30841 acgcttttgc taggtcgata tcaaagccga cgatctggtt attggcatcc atcgactcga
    30901 acggcggata ggtcgctgat acgccaaaat tgattttctc tgcggcagaa gcgccgaacg
    30961 cacaggatgc cagtaatgcg gcaagaacta actttttcat gatggaactc ccgtctgtca
    31021 atcttatgat tattggccgt gtctgcggca tgggataaca atgccatcaa gtgaatttat
    31081 atgcaataaa tatgattaaa taatttgcat
//
LOCUS       DAZKIF010000049        30894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZKIF010000049 DAZKIF010000000
VERSION     DAZKIF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.049"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..108)
                     /locus_tag="ACHOTW_002199"
     CDS             complement(<1..108)
                     /locus_tag="ACHOTW_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844308.1"
                     /translation="MSRHDILLRSQFERIIEGDRVGQALISFYEKLPEEN"
     gene            complement(254..4156)
                     /gene="hrpA"
                     /locus_tag="ACHOTW_002200"
     CDS             complement(254..4156)
                     /gene="hrpA"
                     /locus_tag="ACHOTW_002200"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP9844309.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4357..4962
                     /gene="azoR"
                     /locus_tag="ACHOTW_002201"
     CDS             4357..4962
                     /gene="azoR"
                     /locus_tag="ACHOTW_002201"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP9844310.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5013..6305)
                     /gene="ynbD"
                     /locus_tag="ACHOTW_002202"
     CDS             complement(5013..6305)
                     /gene="ynbD"
                     /locus_tag="ACHOTW_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFP9844311.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6319..8076)
                     /gene="ynbC"
                     /locus_tag="ACHOTW_002203"
     CDS             complement(6319..8076)
                     /gene="ynbC"
                     /locus_tag="ACHOTW_002203"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP9844312.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8092..8988)
                     /gene="ynbB"
                     /locus_tag="ACHOTW_002204"
     CDS             complement(8092..8988)
                     /gene="ynbB"
                     /locus_tag="ACHOTW_002204"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9844313.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8988..9593)
                     /gene="ynbA"
                     /locus_tag="ACHOTW_002205"
     CDS             complement(8988..9593)
                     /gene="ynbA"
                     /locus_tag="ACHOTW_002205"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9844314.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9764..12070)
                     /gene="ydbD"
                     /locus_tag="ACHOTW_002206"
     CDS             complement(9764..12070)
                     /gene="ydbD"
                     /locus_tag="ACHOTW_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFP9844315.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12134..12994)
                     /gene="pdxI"
                     /locus_tag="ACHOTW_002207"
     CDS             complement(12134..12994)
                     /gene="pdxI"
                     /locus_tag="ACHOTW_002207"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP9844316.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(13214..15673)
                     /locus_tag="ACHOTW_002208"
     CDS             complement(13214..15673)
                     /locus_tag="ACHOTW_002208"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9844317.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(15893..18871)
                     /locus_tag="ACHOTW_002209"
     CDS             complement(15893..18871)
                     /locus_tag="ACHOTW_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HFP9844318.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVKHNPVVYKNG
                     VTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIVKSWTVHDDSNTVVFDGVNT
                     SGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAIITDQGGNTALNGGTVMTVDG
                     DNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVDGGTAVIINGDNTKLNTAGD
                     STITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVGDGATIKQEGDLYVSGGAHG
                     IDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMGDLNVGQAAAGENAIGIQID
                     GDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVGDFSTGLDVSGNNNNMTLAT
                     YELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNAQPYFFNPSTGVNISGDNND
                     VTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDDNTFTLNGGVHFVGEKNVMD
                     DGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFPLGFENLIQLSHGAELEIGA
                     DATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVELKNIGFAAAWNKDSTVINNG
                     SIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQHSVLNYPAEWNLSDGTSFN
                     NKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAIDKSTADNEGQITLDTLWVDE
                     NDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATATNHENGVITVYNAGAGMAAY
                     GNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGTAINDQTGVININAENGQAF
                     YNDGTGLIINYGTICTFGVC"
     gene            complement(19285..19608)
                     /locus_tag="ACHOTW_002210"
     CDS             complement(19285..19608)
                     /locus_tag="ACHOTW_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP9844319.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(19616..19801)
                     /gene="ynbE"
                     /locus_tag="ACHOTW_002211"
     CDS             complement(19616..19801)
                     /gene="ynbE"
                     /locus_tag="ACHOTW_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP9844320.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(19798..22437)
                     /gene="ydbH"
                     /locus_tag="ACHOTW_002212"
     CDS             complement(19798..22437)
                     /gene="ydbH"
                     /locus_tag="ACHOTW_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP9844321.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            22645..23634
                     /gene="ldhA"
                     /locus_tag="ACHOTW_002213"
     CDS             22645..23634
                     /gene="ldhA"
                     /locus_tag="ACHOTW_002213"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9844322.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            23745..24167
                     /gene="hslJ"
                     /locus_tag="ACHOTW_002214"
     CDS             23745..24167
                     /gene="hslJ"
                     /locus_tag="ACHOTW_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP9844323.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(24164..24430)
                     /gene="ydbJ"
                     /locus_tag="ACHOTW_002215"
     CDS             complement(24164..24430)
                     /gene="ydbJ"
                     /locus_tag="ACHOTW_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP9844324.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            24704..28228
                     /gene="nifJ"
                     /locus_tag="ACHOTW_002216"
     CDS             24704..28228
                     /gene="nifJ"
                     /locus_tag="ACHOTW_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9844325.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSTDEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            28594..29707
                     /gene="ompN"
                     /locus_tag="ACHOTW_002217"
                     /pseudo
     CDS             28594..29707
                     /gene="ompN"
                     /locus_tag="ACHOTW_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            29848..30282
                     /gene="uspF"
                     /locus_tag="ACHOTW_002218"
     CDS             29848..30282
                     /gene="uspF"
                     /locus_tag="ACHOTW_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP9844326.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT         7882 a         8100 c         7093 g         7819 t
ORIGIN      
        1 attttcctct ggaagttttt catagaaaga tattaaagcc tgaccaacgc gatcaccctc
       61 tattatccgt tcaaattgcg acctcagtaa aatatcatgt ctactcattg caatacgtcc
      121 tgttcgccca aggcgaagta tgaatgaaac ttatgtaaaa tagtaccgcc cttgctatat
      181 ttcagaattc ataaagaaaa aacccggcgc taaatgctta ccgggtttta gatttatcag
      241 gcaaatagca gggttaaccg ctaatctgct ccatcgcctg caaaatacgc ttatctgaaa
      301 tcggataagg cgtaccaagt tgttgagcga agtaactaac gcgcagctct tctatcatcc
      361 aacggatctc tttcacgtct tcatcctcac gacgtgcagg cgggagtttg ttgaaccatt
      421 gctgccacgc ctgctggacg ttttcgactt tcagcatctg tgcacgatcg cgatgtggat
      481 caaccgccag tttttccagc cgtttttcaa tcgcctgcaa ataacgcagc gtgtcgccca
      541 gccgtttgaa gccgttacca gtgacaaaac cgcgatatac caacccgccc atctgcgctt
      601 taatgtcaga aagccccagc gccatggtca tatccacccg ccctttcaga cgtttgttga
      661 tattgaacac tgccgtaagg atttgctcga cctgcttcgc aatatccacc accgtgtcgt
      721 tcagttcggc acgcactttt tcatgcagcg cagcaaagcc ttcttccgtc cagaccgggc
      781 ctccattggc gtcgatcagt tgatccacac cgcatgagat acagtcgtcg atcagctcca
      841 gcactttgcc atacgggtta aagtacagcc ccagcttggc tttgtttggt aacttctcat
      901 gtaaatactt gattggtgag ggaatattca gcagcagtag acggcgaaga ccgttccaca
      961 ttgcctgttt ttgctccagc gggttatcaa acagtttgat cgccacactg tcgcgctcat
     1021 ccaccagcgc cggccacgcc ttcactttgt agttaccacg cttctgttcg tagctttccg
     1081 gcaactgacc aaaactccag atatgtaagc cgctctgctc gataccgtca tccgccaccg
     1141 cagatagcgt ttcctgcact ttgcctttca gcgcatcttt cagatcttgt agcgagcgcc
     1201 cttcttttag cttcttgttt ttgtcatcca ccacgcggaa ggtgattttc aggtgatcgg
     1261 gcacctgatc ccagtgccag tcttcgcggt caacggtaac gccggtcatc cgccgtaact
     1321 cgcgctcaag gctgtcgagc aacggtaact ccagcggtgt gacgcggcct aaaaacgctt
     1381 cggcatagtt tggcgcaggt acaaaattac ggcgtaccgg tttcggcaac gatttaatca
     1441 gagcaatcac cagttcgcgg cgcagaccag ggatctgcca ttcaaacccg ctttcctcaa
     1501 cctggttaag taacggcagc ggaatatgta cggtcacacc gtcagcatcc gcgccgggct
     1561 caaactgata gctcaaacgc agcttgagat tgccctgatg ccagaagttc gggtaatcca
     1621 gcttgctgat cttttccgcc ccttctttga tcaacatgct cttttcaaag ttgagcaaat
     1681 caggcgtttc gcggctgact tttttccacc agctgtcgaa gtgacgagcg gagattacat
     1741 cgtggccgat gcgctggtcg tagaactcaa acagcgtttc gtcatcaacc agaatatcgc
     1801 ggcgacgtga tttgtgttca agctcttcca cttcggcccg cagtttcagg ttttcacgga
     1861 agaatgcgtg acgcgtctgc cagtcacctt ccaccagcgc gtggcgaata aagagttcac
     1921 gacataacgc cggatcgatc tggctgtagt tgaccttgcg cgcggcaaca atcggcaaac
     1981 cataaacagt gactttttcc gttgccatca ccgcgccctg cgcccgttcc cagtgcggtt
     2041 cgctgtaggt acgtttaatc aaatgctgag cgacaggctc cacccattcc gggtcgatac
     2101 gcgcagcaat gcgcccccac aggcggctgg tttctaccag ttccgccacc attacccatt
     2161 tcggcggttt tttgaataaa ccagaaccgg ggaagatgga gaaacgcgcg ttacgtgcgc
     2221 cggtatattc ttgtttatcg gcatctttca tgccgatatg ggaaagcaac cccgtcagca
     2281 acgcaatgtg aatttcacga tactccgccg gttcgctgtt caccggaatg ccgagttctt
     2341 tcaccacctg gcgcaactgg gtgtagatat cctgccattc gcgcacacgc agatagttga
     2401 gataatcggt acgacacagg cgacggaagg cgttggaaga aagcgccttt tgctgctcgc
     2461 caagataatt ccacagattc acaaacgcga ggaagtcaga ctctttgtcg tggaagcgac
     2521 gatgtttttc gtccgatgcc tgctgtttgt ccatcggacg ttcgcgcgga tcctgaatgg
     2581 agagcgcaga cgtgataatc atcgcctcac gcacgcagcc atgtttttgc gcttccagca
     2641 ccatacgcgc cagacgtggg tcgacaggca actgcgagag ctggcgaccg agcggcgtca
     2701 gtttataggc gctggcctgt tcatccgtgg tgatcgcgcc cagttcttcg agcagacgca
     2761 cgccatcctg gatattgcgt ttatccggtg cttcgacaaa cgggaacgca gcgatatcgc
     2821 ctagccccag cgcggtcatc tgcaaaataa ccgaggccag gttggtacgc agaatctccg
     2881 gatcggtaaa ctccgggcgc gagaggaaat cgtcttcgga atagagacga atacagatcc
     2941 cttcggacac acgaccacag cggcctttac gctgattggc agacgcctgc gaaatgggct
     3001 caatcggcaa acgctgcact ttggtgcgat agctgtagcg gctgatacgc gccgtaccgg
     3061 ggtcgataac gtatttaatc cccggtacag tcagcgaggt ttccgcgacg ttggtcgcca
     3121 gcacaatgcg tcgcccgctg tgcgactgga acacgcggtt ctgttcgcta ttcgaaagcc
     3181 gcgcataaag cggcaagatt tcggtatggc gtaagttcag cttgttcagc gcatcggcgg
     3241 tatcgcgaat ttcccgctcg ccgctcataa agatcagaat gtcgccgggg ctttcctggc
     3301 ttagttcgtc cacggcatcg aaaattgcct gcaactggtc gcgctcagtg tcgtcggctt
     3361 cttcaacaat cgggcgatag cgcacttcca ccggataggt ccgaccggag acttcaataa
     3421 tcggcgcatt attaaagtgg cgcgaaaagc gttccgggtc gatagtcgcg gaagtgataa
     3481 tgatttttag gtcaggacgc cgcggcagca actctttcaa ataaccgagc agaaaatcga
     3541 tattcaggct acgttcgtgc gcttcgtcaa taatgatagt gtcgtactgc atcagcaggc
     3601 ggtcttgctg gatctccgcc agcaggatac cgtcggtcat tagcttgacc atcgtgttat
     3661 cacttacgtg atcgctgaaa cgcactttgt aaccgatgca accgcccggc tccgttttca
     3721 gctcttccgc aatacggttc gctaccgttc ttgccgccag gcgacgcggc tgggtatggc
     3781 cgatcagccc tttaatcccg cgccccagct ccatacagat tttcggtaac tgagtcgttt
     3841 taccagaacc cgtttccccg gcgactatca ccacctggtg atcacgaatc gcttcgagaa
     3901 tgtcctgttt tttctgacta accggcaaat tgtcaggata agtaatttcc ggtcgtgccg
     3961 cttcacgcag caggactttc cctgccgcct ggtcaatctc tttcgccatc tcctggaaaa
     4021 tggcctgttg tgcatcagga tttttaacct tcttcacgcc gtgcagacgg cgagaaaaac
     4081 gcagtctgtc acgcagcatc agcgaatcca gccgctgctg caaggccgta aaagtcaatt
     4141 tttgttgttc tgtcataacg ttagtgggca gtagtctgcc tgattaattt tctttgaaat
     4201 gatagatata gagtaccaca tcgcggcttt ctatgcgttg ttcaataaat tcgaacatag
     4261 gcttcgattt attgcgctat ctctgtggca ggattgtgaa taaagtgtca acaagcaacg
     4321 gggcatcgcc cattcaaata tctataagga aacaccatga gcaaggtatt agttcttaaa
     4381 tccagcatcc tggcagggta ctctcagtct aatcagttgt ccgattattt tgttgaacaa
     4441 tggcgcgaaa agcactccgc tgatgaaatc accgttcgcg acctggctgc aaatccaatt
     4501 ccggtactgg atggcgaact ggttggcgct ctgcgtccga gcgatgcgcc gctgactacg
     4561 cgtcagcagg aagctctggc actttccgat gagctgattg ccgagctgaa agcccatgac
     4621 gttatcgtta ttgcggcacc gatgtataac ttcaacatct cgactcagct gaaaaactat
     4681 tttgacctgg ttgcacgtgc aggcgttact ttccgctata ccgagaatgg tccggaaggt
     4741 ctggtaacgg gtaaaaaagc catcgttatt accagccgcg gtgggatcca caaagatgga
     4801 ccaacggacc tggtgacgcc gtatctgtcc acgttcctgg gctttatcgg cattaccgat
     4861 gtgaaatttg tcttcgccga agggatcgca tacggtccgg aaatggcagc gaaagcgcag
     4921 tctgacgcga aagcagccat cgacagtatt gttgctgcat aaccggacac gatcttgatg
     4981 tggactgtag ttcagtccac atctcaatcc acttacctgt tttcccataa tttcaacatc
     5041 gctttgtgct cttccttaag cacaatatgc gagcgtctgg ctcgaataaa gctaatcgct
     5101 tcatcaactg ttttacagtg tccgtaacat aacagccatg ccgccactac cagcgcactg
     5161 cgcgataatc ccaatgcgca atggaccaga acgctgcctt gctcttcgcg taatgtttcc
     5221 agcatcgcca cggcctgtcg gagctccccc tcttccggaa ccaccagatc cagcatcggt
     5281 acacagaaat agagtcgatc ttttgtcgct cgtccgcgag ggaattcaaa ggtgacatcc
     5341 agaaccgcat tctgtgccgg aatatgtcgt ggaaacgccc ctaaataaac accagcagtc
     5401 attttgctca ccggctccag gcgacgacaa aaccaacgca tagatagcca catcccgatg
     5461 cgccagggca atgtcagcca gtaaacggcg ggcgggagtt tcccctgact atctttccct
     5521 gttgttatcg cgccaagccc accgtaccca cggccaataa tgagtagcga taatactggc
     5581 caacataacc agactgacca ccataactga accatcatca ttagctccat caacacaatg
     5641 cacgcgcacg cgcctacgac atatggcagt gctattttga ttcgacgttg atcaggtttc
     5701 tgataattcc aacgacggtc gacgggtatc atccagtcaa tcaacatacc taccgccagc
     5761 cctgtgatga catcaataaa atgatgctgc caggttgtca gcatcgaaat ggcgatgagt
     5821 aaaaaccatc cgccgcagac tttacgccac ctcacagcca gatgctgacg aaagtgacgc
     5881 caaagtagcc agcagagaac aatatgcagc gaaggagact ggttataagg cagatcaaac
     5941 agttcaagtt gcgaaaatag ccatcctgtc accccactca cttcaggacg gataaaacta
     6001 aacttcagcg ggtagagaaa aaaaccgcag caggccatta ccgttgccag aataagccgg
     6061 tggacaagtc ggcgctgttc gaatgtcgag ctacaaacga acagcgaaaa tccatataaa
     6121 agatccagac tccagtaagg gacaatagtc cagggaagaa aagggatcgc cgtttcccag
     6181 ccgaatacct gactggggat atcatggctg ttaaagtcct gaaccgcggt gaactgattt
     6241 agagatccat aggtgaagaa gaaaaacggg gccagcaaca ataaccagcc agcgccttgt
     6301 agcaatacgt tccggcgttc agttatcacg acgcaccgcc atcgaaaccg taaaaatgcc
     6361 ccactcatca atccgttgtg tgcatttatc aaatccggca tcacgcacga gtgaatccat
     6421 ctccccttgc gaacgcacgc gcattaccca cggtttacca tctttatgac tggttaacac
     6481 cccggcaatc agctccagtt gtgggtgcca cggctgcccg gtgtagagga gaatgccgcc
     6541 cggttcgatg gcattggcaa gacctgcgag tgagtttttt acctgctcat ttccgggaaa
     6601 aagctcatac agtccagaga caatcgccag cgtaggccgc ggagttaacg cgctgagttc
     6661 ctccgggtta aacgcatcgc cctgttcaaa acgcacccgc ccagacattc cccgttgagc
     6721 aatcatctct tgcccctgtg caacatttaa ctcgctgtaa tcacgtaaca aaatatcgct
     6781 tacggcaggc tcgtttgcca gcgcatccag tacatagcgc ccatgccctg cggcaatgtc
     6841 aaccacgcgg acggctaaac ctttggcgtg gagatcggca acggcttgtt taatcagtat
     6901 ttgtaaatgg gttttgcgct ggcgaatacc gcgccagcca acactgttca ggtagatttt
     6961 gtcgattaag cgcccgaatg cgttactacc ttgcggttga ttttgataga cataatccag
     7021 cgaactgcct gaatcaaagc cggtgctcat tccgagatgc aggcccgcag agtgcgtccc
     7081 gaacaatttc atcgctttac gcataaagcg atacgccaaa tcaaccggcg ataatggcac
     7141 gggtccacct gaaaggagcc gccagcgatc cgctgatggt ccagtgcggt cttcatgctg
     7201 ataatcaaat ttttgtgact tgttagcata taaacgacta ataaagcttt gcattttttc
     7261 aaatgcctgc gccctgttct cttcacccaa cgtgtcgtga taaaagcctg gcagcagatg
     7321 cagctctttc agagggctac gtaatctatg ataaaaatca atctgcggtt ggcgatgcac
     7381 cacatagtca tcgcctgata tcagaagttg cgtggggagc gtaatcgccg ccgcatcgct
     7441 aacaatacgt tcagacgttt tataaagatc gagcaagata ttaacggcaa tcgcccgtgt
     7501 gatcagcgga tcattattga aactcgccac ccgttgccga tcgtgggtca aatagcgtcc
     7561 tttcacatag gaattaataa aaaacagacc acgcagacga tgccataacg ccagcgcagg
     7621 acgtgccagc ggcacataca atttaacctt aaaggccgga gaagccagca ccagcccgcg
     7681 aattgcaggc gcataatcat gaacccatgt ggcaaccagc actgcgccga cgctctgcgc
     7741 gatcactacc acctcttcca gcccgacctg gctgtcgctg gcagcaaaac ggacaaactc
     7801 atcgacatcc cgcactgaac gcgcaagaga tgggctataa ccacggggcc ccgaagtttg
     7861 tccatgccct cgggcatccc atgcataaaa agcagtatct ggcatcgcca gttcatcaac
     7921 gatatgttgt agacgaccag aatgttcatg accgcgatga aataagacga tgaccttttt
     7981 cgcccctggc tgtaaagcgg gccagtgccg ataaaacaac gctgtattat cactggtggt
     8041 aaaaaaatgt tccccaggga tgcgtgaatt ttccattttc tctattttcc ttcagtaaca
     8101 gcagtagcga ataaaataaa aaaataccgg agcggtgaaa attaatgagt caatcctgtc
     8161 aaggagtccg ccatgtcctg gtaatagttt tccgctatct ttaacaccca catctcgttt
     8221 gacggctgac atcactacat cgccgcaaaa accactaata ccaattaaca aacccgctaa
     8281 taatgcctgt aatgtattta gcggtgtcag caacggtcca ataatcagtg acgcaatcat
     8341 ggtggtgatt acgcccccca ccagaccttc cagcgttttt cccgggctga ctttggggac
     8401 cacttttctt ctgccgcagg atttccccca taaatactgt gcaatatcgt ttgactctgt
     8461 taacgccaaa agaaataaca ccagtaacgc cccgccctgg gtatttgtgg ttgtcaacat
     8521 taataaccag gcggcatgac tcaaggcgaa aacggtcatt atccagcccc aaaaaatggc
     8581 gctcacggta tgcagaaaac cggaggtatc acctactaaa actcgccagg tggttaatat
     8641 cagaaaccca gccaaaggta taaataacag aaacagctca aagcaactaa aaccgataag
     8701 caaatagtta agaggaataa cccaatataa ccaacgcgga aaatgtacgg atataagcgt
     8761 acaatattct ttcaatgcca gaaaacttat cagcgcaaaa aacgtcaacg tcatccagcg
     8821 tggacctgaa atcaccagtg aaaaacaaat gacgataccc caccaggtac gaatacgtag
     8881 tgtaacttca tccccttttc tctccggtaa ccgccatacc cgaaaacggt ttatcagcgt
     8941 tgctaaaatt aacaacgcga aaagtgttgc cagagatttt tccagcatta tctttcagca
     9001 ctcatcagga gcacactccg acaacgatta atcgcagtcc agagaagcag aattgaagca
     9061 atgctccata aaagattatt ccattgcatc cactgtggat aaatggcaac ggccagcccc
     9121 cacaacccaa atattaacgc gcgatcgctt ttgccaaaag gaccgacata actacgaata
     9181 ccgttaatcg tctgcgcgag taaaccgcaa aactcagtca atatggtgca aaataacatg
     9241 agtattacga gtgatgcgtt actttccggt aaaaataaaa acggtaaata gagcgcaata
     9301 tcggaaataa catcgccagt ttcatttaaa atcgccccca gacgtgtttg ctgattgcac
     9361 tcacgcgcca acatgccatc cagcgcattg agtgccatac ggataaaaag cacgatgggc
     9421 aatagcaaaa agaggatggg ttgtgcgacc aacatcaaca gcaatccggt aataagagaa
     9481 agcgccagtg cagcaagagt gatgtgattc gctgtaacat ggtgcttata aagccaaaac
     9541 atcgtcggcc ttaacagcga ctgaaagagc ggttttatct ggtagagcgt cattatcaat
     9601 ccttgataat tcggtttgcg ctatggtgca tcaattcccc tgacggagat atcgcaacac
     9661 gacgcttcat taaaaattca gaatatatca aggtattggg gactcactgt caggaatgat
     9721 tatccccaat aaatcaagtg gttaaaaatt attacgctgt tattcaatac aactcatcaa
     9781 gttttctcat cgcttgttcc cgtattgcgc gcgtcttacc gttaagcgcc agctggcgaa
     9841 ctaaatccag ttcagggtta acaagcttta acgccagcaa aggatcacgt ttcagcctcg
     9901 cctttaatac atcaactgga aaacggggat tattcatcgc cacaatccac cactcgggat
     9961 caatttctgc cgccagtact gcaggcggtg tgtggcggtt gttaagtgcg ccgaacttga
    10021 aaaatgtgag attgcttttc gccagtgtac tccaccatat acgatccggt tgttttgcca
    10081 gtacatccag ttgtgctaac gcatcggggt ctagtgcata ataaacacgt aacgcttcgt
    10141 taacctgcgc acgggaactg ttttcctgcc tcagaaaaaa cttcgcccaa atatcattgc
    10201 gtggcgtgtt ttgctgtaat tccttgagaa caacagcggg taaattatcc caactaatta
    10261 atgcactctc taaaaagtcc cagtcgtccg aacgtatcag agaggtggca tttaatagca
    10321 tctggtcatc aacttcaccg tagctgataa gctcagacaa aatcatatca gcgctggttt
    10381 catatgcatc tgaatctgct ttgggattat ttttccgatg cgataaagca cgttgccaaa
    10441 ttttttgttt catcgacgtg ctcagatact gatttttcgc cagattactc cacccctcag
    10501 gatcgttgaa acgtgtcaat aaccgttgta aagcagcgtc agagtgaatt atctgcatat
    10561 cccgacagca aaagtaggca ggggattttt tctccatcct tttaacaaaa ttaaactgta
    10621 agttatctgg caacgctaaa aataacgcac ctattagatc atcgtcattc tcgaattttt
    10681 ggtataaata tactgctatt ttgtcactct cttgtggcgt catccgcaac aattgaccat
    10741 ggtgtttcat ctctgccagc tttaacgcta agcttgtgac aacatcgttt gatgaatcgt
    10801 tcgccaactg cattgcctga ttgtaggtga gttgtcctcc agcaccgacc tgacgaatag
    10861 aggaatcatc actgcggata tagccatcaa taatggattc gggcaataac cacgcatctt
    10921 tataaagctc ctgaatgatc atatcccaat ctcttttcgc cagagtcatg acgatatcca
    10981 gagggagatt ttcacgtagc gcaagattca acggaccgac accatcaggt agatgactca
    11041 ttcgcgtcat tatttcgaca gagagcggtt cacggtcgag caaccccaga acaacaggct
    11101 ggtaacccaa ttgctgatcg gtcaactcca gaatctcatt gtcaggcata tatctcgcca
    11161 tactttcaat cgtttgtcca tcattgctat taataagttg tttgcgtagg ttagctgaaa
    11221 ttcctttaag ccttgggaga gttcgcatta tggcaagatc aaatgtcgca atatctcgtc
    11281 cttcaaatgc agcaacgttg ccttcatcca gcatttgcaa ttcctgcact aactcaggag
    11341 aaggcttaag gcgaaacagc gcttcttttt tcatctgatt acagacattc cctgccatgc
    11401 gaatattgct tcgcttaaaa atttctttca gagcatcttc atcatttatt agaccccaat
    11461 aatatgcatc aggatttatt atcatcagcg cttttttgac attttcagtg tcatttttgt
    11521 cactcgcacg gaccatgaat tcactatttg tcgaagacgc aacaacaggt gcaattatgt
    11581 cattgataaa tatcccacgc gcattaatac tccccttaat cgtagacgga ttatcgtaat
    11641 atgagtcgat aaataccagt ccacttgcat ttatcgaacc ggtaattaac agtggtgaat
    11701 ccgtaactat attcgttgcc gtcacgttac cgagaataac ccatggatta gaaagagaga
    11761 aagtggaaat attacctttg actgtcaaat tgccaagaac aatcaatttt tgattactct
    11821 ctatattacc ttctaccact aaatcgccac gatgcacttt ccagtccgat gcagataaac
    11881 cgaaggggac aagattcacg ctactgttga tagcttgttc aacttgttgt ttatcttcag
    11941 cggtagccgc agaaaaaaaa ggttcaatat caactgcatt tgcaacgcat cgaagttccg
    12001 cctgtactgg aagactcagc ataaacaggg gaagtaaacc tatccagctg caaagcctgg
    12061 gcttaaacat atgcactcca tgccattaaa aaagaaacaa aagggaaatg catctttccc
    12121 ttttgtatat tcgttattct cgcgaaatac catccaacgt agacaacact ttctcagaaa
    12181 gatgcaactt ttcagcagcc atattctccc gtaaatgcgc aacagaagac gtccctggga
    12241 tcagcaaaat attcggtgaa cgttgtaaca gccacgccag cgccacctgc attggtgttg
    12301 cacccaagct cgcagcaaca tcggaaagcg tggaagattg cagcggcgta aagcccccga
    12361 gcggaaagaa cggcacgtag gcaatgccgt cacgagccaa agcttcaatc attgcgtcat
    12421 cagcacgatg agcaatgttg tattcgttct gcacacagac gatttcggca atcttgcgcg
    12481 cctctgcaac ctgcgtcggt gtgacgttgc ttaggccaat atgttttacc aggccttgtt
    12541 gttgcatctc tgccagcacg gtcaggctgg cttcaatcga tccttccgcc gggccatgac
    12601 catcccccat catcacgcgc aggttaacca catccagtac gtccaggccg agattacgta
    12661 gattatcgtg caccgctttt tgcagctctg ccggagaaaa tgcgggcaac caggatgcgt
    12721 cctctccacg ccgcgcacca attttagtga caattgtcag atcgtcagag taaggataaa
    12781 gcgcttcgcg gataatctga ttggtgacgt gcggaccata aaagtcgctg gtatcaatgt
    12841 gattgacgcc caatgccagc gcctcacgca gcacggttat agcgacgtga cgatctcttg
    12901 gggggccaaa aaccccagga cctgccagtt gcatcgcgcc ataaccaaga cggttaacgg
    12961 attttgtacc gagagtaaat gtattgctgc tcatcgcacc ctgccctgta ataaaacgga
    13021 ttaagaaaaa tcagtataga aacgaaaaga gataaataat gcgaaggcgt aaacaagatt
    13081 tctggatgag tagcactatt gtcctgaaag acaccaaaac attagacagc aaaagataag
    13141 tcaagcgtcg catccggcaa taaaaaaccg caacctgtat cagcgttgcg gtttgtgtat
    13201 cacatgctaa aaattagaac gttttcttaa agttcagcat cacgcctttg tcgctgatgc
    13261 cgtcctcttt ccagtgatac gcatccagat tcagcgaact ttctttgcta ctgtacttca
    13321 cgccgacgct cgccaggctg ttgataccgc cgcccttctg accgtcatcg acgttaaagt
    13381 gctggctgcc tgcccctgca aggcttgccg tgcgctggct cttgctgtag ctcaggttcg
    13441 gcccgccttc cagcgtcgca ttcgcgctcc agccgccttt ccctgcgtag tccagtttca
    13501 gcccgacgat gctgtccacc gccgtttcgc tgccgctgtt catgctcagg ttaaagtctc
    13561 cggcattgcg ctcctgatag ccgccttcca gcgagtgacg cagtttcact ccggcgtacg
    13621 gggtgatttt cagcccttcg cgcggctcaa aggttttcgc cccttcgcta cgcaactcca
    13681 ggtactgctg tttcacatcc gtatccgccg ttttgctgac gtcgccgtaa gcgaccgagc
    13741 ggctgctgtc gagattatgc acgtcataac gcagcgcgtt gttccaacgc ataccattgt
    13801 cgaaggacat ctggtgcttc aggccaaaga actggctgta gccgccggtt acgccattgt
    13861 cgcccgcttt ctgcgcaccg tcgccatcca gacgcgcgat accgtattcc aggctcatgc
    13921 tctggctttc gctcaggtca acggttttac gcagtgccag catgtcatac tcggtgttat
    13981 ttccgagttc cgcacgcggg tcacctttcg ccaccacgtt aaaggccagg ccattgccca
    14041 ccttcggcgc ggcatccgcc agcatgctga agcggttgct taacacacgc gcttcacgga
    14101 ataccgtggt cgcctggcta ccgctcacct gcttcagggc gctattcagt tcagcagtgg
    14161 tgcccacgtt caggctggta tacagctcgt tattggtgta acccgcatcc agtgccttcg
    14221 ccacatcgtt caccgaagca tcggtcgcca catcggtgta ggcgtttttg ctcatgatga
    14281 cgtcaacgtt gccgctggca tcggtgctgc ctttggcggt ccataccacg gacgttgagg
    14341 tgatggcatc ggcgtcggtc aggttgatgc cttccaccac gttgtcgaaa ctgaccgtgg
    14401 tgtctgccgt accggcggcg aaaccggtgt tgatcccgac accattcatg ctggcgttgt
    14461 tgaccttcag tttaccggcg ctgccgtcaa cgttggtacc gacgatatag ccgttgaggt
    14521 tgttactgct gccatcgcta ctaccgctat cagtcgtttc actgaaaggt gaagtggtgt
    14581 aagtgtttgg catgtccggc aacgtgtagt cggtgtaatg cacttcggta ttgttaccgc
    14641 tgttgccgtt caccccttcc acattcacgc tgtcgccctg cttaatcaac cccgaaccag
    14701 tcacgccgtc ggcaatcacc accgtaccgt tgttaacgat atgaccttct gtgcccagta
    14761 cgctgaacgc aaacgagtcg ttagcaaaaa tattgatcgt gccgttgttc tcaattaccg
    14821 catcggcggt ggcattggca tccagttgca taccagtcaa tccggtatcg gtggtgcctt
    14881 cggtaccgag attgataacg ccgttgttaa ccagctgtgc gtttttatcg ccttccatcg
    14941 ccacggcgtt gttgccgctg atgttcatta caccatcgtt gagagcaaca tagttgttgt
    15001 gggtgaattt aaccgcgctg gcgttgtctt tatcgaagtt aattaccccg ccatcctggt
    15061 tccacaagaa ggcatcgctt tctgtcgcac cggcagtact taccgccacg gtgactttcg
    15121 catccaccac gcccttgtta ataaactcgc tggcgccatc gtcgtagtga atagcgcccg
    15181 cacctaatgc ggcatccagc gtcatttcac cggtgttata taacgtggcg ttagcattgc
    15241 tgccctggaa gcgaatggcg tttttgctgt tatcaaggat gagtttgccg ctgttgttga
    15301 acgttgactt atcgtcaata tccagcgtcc cggcattgtt gttttcatcc tgataaccca
    15361 ttttccaggt acccgcgtta ttcactaccg cagagttata gagaatcgcg cctttacgcg
    15421 cctcgccgcc tgcaaggtta ttgaataccg catcgccctg agcgttaacg cgcgccgacg
    15481 caataccgct gttattcacc gtaccatcgt gaagttgtaa agttgacagg gaaccggtgt
    15541 tattaattac cgcgttgctg tttttctcaa tttttacgac gtcattaagc gttccgttgt
    15601 tattgaaggt tcctccttcc agagtcaccg tgttcaatac accttcattg gttaacgcac
    15661 cgccttcatt cacgacgatg gctgagctaa tggttccgct cgactcgtta accagcgcac
    15721 cattttcgag tatcctgatc gttccatttt taacagcacc cgcactggtg acatccccgt
    15781 tgataatcgc acctgacgat aataatacgg tctcacccgc agaacttaat acagcgccat
    15841 catcatataa atcagcaata taattatccg ttggattata ttcatcggcc ccctagcaaa
    15901 cgccaaaggt acaaattgtg ccatagttga taataagccc agtgccatcg ttatagaatg
    15961 cctgaccatt ttccgcgtta atgttgatta cgcctgtctg gtcattaatg gcggtgcctt
    16021 cgtgataaac cgccatgccg acgagtttat tcacaccgag agaatcgttg taattttcat
    16081 ttttctccag gttgatggtt ccctggttaa tgaccgtatt agtattccca tacgccgcca
    16141 tccccgcacc ggcgttgtag acagtaatca caccattctc gtggttggtt gccgtggctt
    16201 tagttaatgc cccattatag gcatcggtac ctgaagccat tcccacaccg aaatctttcg
    16261 ccgtgctatt tgcaacatta ttacgcagcg atgtttcatc attttcatca acccacagcg
    16321 tatccagggt gatttgccct tcgttatccg cggttgattt atcgatggct agcatcccta
    16381 cactgccacg gccatacata tcaataatac ccgtttcgcc gttcagaatg gatgacgagt
    16441 agctggcaag catacctgtc agccctaatg ccttgttatt gaaagaggta ccatcagaca
    16501 agttccactc tgccggataa ttcagcacac tgtgctgatt catcattttg gtggtcatgg
    16561 taccattgtt aacgccaatc ccaccgtatt caacatcaaa gacttttggt gccgggtcgg
    16621 taccaaaatc gtacatcgcc aggccaatgg atccgttatt gattaccgtt gaatctttat
    16681 tccaggcagc cgcaaaaccg atatttttca gttccacttc accgttgttt gtcgccttcg
    16741 caccagaatc aatactaatg gtggataacg ctacacgata ataataagtg aaagaatcca
    16801 catcactcat atcaaacgtt gcgtcagcgc caatctccag ctccgcgcca tggctcaact
    16861 gaatgagatt ttcgaacccc agcgggaact cgccgctgat ggttgactca ccattcaggt
    16921 agaccgggga atgcccatca acgttaataa gcggtgtgtc tccgatacca cgtcgagaag
    16981 ctgatggttt tgagccatca tccatgacat ttttttcacc gacaaaatgg acaccgccat
    17041 taagcgtaaa ggtattgtca tcgccagcaa taacaatacc tgcaatgtgt tcctgagcac
    17101 cgtcgtaact cgcataggaa cgcgacgtgg ttttactatc agcaacaacg gttaactgac
    17161 catccagcgt gacgtcgttg ttgtcaccgc tgatatttac cccggttgag ggattaaaga
    17221 agtaaggctg cgcattatcg gctttctgat ctttatcaac cagaatactg cccgctattt
    17281 cgatagtatt accatcaccc gaaacattaa cacccgtcgc tttttgccct gtcacattca
    17341 gctcataagt cgccagcgtc atgttgttat tattgccaga gacatcaaga ccggtggaga
    17401 aatccccaac gtctaaccca cccgcgagtg aaatattagc tttatcccca gcaacgctga
    17461 caccggttcc ggcattggtc gcggaaatat caccaacgtt aacaaatgtc gcattatcac
    17521 cgtcgatctg aatgccaatg gcattttcac ccgctgcggc ctgaccaaca ttgaggtcgc
    17581 ccatgttgat cacgctgacg ccatcaccat caagcagcat gccaatagag ttatcttcga
    17641 tgaccgtaat attacctttg ttgctaacga acgtatcatt gccgtcaacg tcaataccgt
    17701 gcgcaccacc gcttacgtat aagtcgcctt cctgtttgat ggtggcaccg tcaccgacaa
    17761 ttttcgagcc agtggaattc tctccgtcaa cgctcatggt accttgatta tcgacccgcg
    17821 cattatcgcc attgataagg ctacccgtac caccattggt gatggtcgag tcgcccgcag
    17881 tgtttagctt ggtattatcg ccattgatga ttaccgcggt accgccatca acttccgtct
    17941 cgccattatt gttaatggtg atattgtcgc cagtgagtat accgacaacc gagccttctc
    18001 cgatggccgt cgttttgccg tcgttgttga gggagatgtt gtcaccatca accgtcatga
    18061 cagtaccgcc attcagcgcc gtgttgccgc cctgatcggt gatgatcgcg ttaccaaact
    18121 caatcgcatt cccggttgtg ccgtcaacaa cgacgccggc ctctttagtg atgtggatga
    18181 gtccatcatc gtcgtaactc caggtgatgc cacctgaggt atttacgcca tcaaagacaa
    18241 cggtgttgct gtcgtcatgg actgtccagg acttaactat cgtctccttg ccctcaggcg
    18301 ccgttaaggt gtagctccca tcggcatttt ggctgtaggt aaaggtagtt tcgcgaattt
    18361 tgacagtttt tgcatcctga tcccaagtta cgccattttt atagacgaca ggattgtgtt
    18421 taaccggggt atcgccacca tcgtctggcg gaactggcgt atcaccgccg tcatctggcg
    18481 gaaccggcgt atcaccgccg tcatcaggcg gaactggcgt atcaccgccg tcgtctggcg
    18541 gaacaggcgt atcaccgcca tcatccggaa ctggggagtt attatggtgg tgattgtcat
    18601 cgtcacttaa ggtatatata gcaattcccg tagctactgc agcaagacct gcagctgccc
    18661 atggtaaaac tgcactatct ttattttctc tgcactccgc ggataaattt tctagctgct
    18721 ctttgctcag ggaatgaata tttgccggac atgttatctt actggactta cgtgtttttt
    18781 cggttgagtc gggtattgaa gtatccgcag cccatgtctg gctacttaac gccatggcaa
    18841 cacaaacaga caataatttt ttcctttgca tataaaattc cttagatcta gaccgtgcgt
    18901 aacaagccat gcagaatttg aagataaatc gtagtaaata ttcatagtga aataagcagt
    18961 aaagaaatgc ttatataatt cacaaaatca atattaaaat caggcaagta aaagcataca
    19021 aaaaaaacag ttgatggaat tatattccac aaaaaactca cttcaatcta aaaattaacg
    19081 acaaaaaaca aaaacacact aataattcat tgacgctgca tatatatttt gtgattatat
    19141 atatattggt aatgatttgt tatttaaggg aaggatatat aaggtttttg tagaaatacc
    19201 cacggtaatt aagcatgcta acgtcagttt aaatactgta aaagatcatt acattaatag
    19261 atacattttg tagccaacta taatttattt gcgcaaccat ttaccattaa ctccctgaac
    19321 atattgcccc gacttcgcgc gagcaacaag cttttgtcca gccagtttgg ctatctcttt
    19381 agtggataca ttattttgct ttgccagttg ttggtaacta tggttacgag cttcattaat
    19441 atcttttact aatgcctgtg tttcggcatc cgtttgtagc gccaccaggt aaccgttaag
    19501 cgtttcacct acccgcccct gacttctggc ttcgttcagg gttaaagcca ttgcactgtc
    19561 gctcatcagg ccaatggccc atacagctat gagtaatgtt cttttcatca tcacctcaaa
    19621 atagatcgct acgggttttg agcaggtctt caacgtcctt atccgcttta atgatgattt
    19681 catgctcaat tttaacgttc atattaatgg tgattggctc tttcggcgca gccacttcaa
    19741 tgcgaggcgt gcaaccaacc aacataaaag aggacgcaaa cgcggctatc actattttca
    19801 ttgtttttcc tcacactctt ttcctgatgg gcagccagtt tccggtagct gggcattctg
    19861 ctccagccat acctggagat tatcgccaaa gcgtaaactg cgccataacg taaaaatatt
    19921 ctcttcatgg tgataattaa ggtttaccgt accgctttta ccatcgacgc gactggtgcc
    19981 cgaaatgttg gcctgcatgg ttaacacacc gagattatct aaattaatcc ttgttgatga
    20041 gcgactgatt tccatataac gcaaccagtt gatggcagtt ccagcggtca tattgtcttt
    20101 ggccaccgca tctgcagtgt ctttatccag acgcagcgtc atcggccccg cgtttgccaa
    20161 ccagccatct ttgacaatcc atttttcatt gttcagccat agtggcaacg ccccactaaa
    20221 tgccccagac atagcaaact gtttcggatt aaccgcgcta accagttcgc tggatgagag
    20281 attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt tcatcagcac
    20341 gttaccgcct aacacatcga cgctaacatc agtcaacagc aatggttctt cttcagtcca
    20401 cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat taatcacttc
    20461 ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat cggcaaaacg
    20521 gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg cactaccgcc
    20581 tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag aaaatgcaac
    20641 ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca ccagcggctg
    20701 gaatacagtc agtgattgtt taggccacca ggcgttaccg cgcagacgaa taccgtccca
    20761 gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt taaagaggaa
    20821 atacgtcgga tcgcgcccat cgacgctaaa ttttaaggtt gatggcggta acacactgcc
    20881 gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg agggatgctg
    20941 tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt ctaccgtcat
    21001 cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa tcgtgctgtc
    21061 atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa aactcccttt
    21121 tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg ccagcccatc
    21181 catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg cctgcaaacg
    21241 tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga tttcatcgat
    21301 gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct caaaggtcag
    21361 cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata aaatgaggtc
    21421 cgcctgtttc gcttcaccgg tcagctgcag aggcagctgg ctgttatcca tgcttaattt
    21481 tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg tcagcacact
    21541 cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga ctttgacacc
    21601 cagtcggcca ctcaaaggaa aacctgcata cggccagctc cagcgaccat cgcttacggt
    21661 caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt tatcccgtgc
    21721 cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca ccagtgacgg
    21781 ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc catccggcat
    21841 gagcggcata gtaaattccc ccaccagttt aaccgacggc tgattttcaa acgcgacgac
    21901 atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt taactttttc
    21961 gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt ttccctgcca
    22021 ttcctgccag ggagaaaaaa tcagtttatc aatattgatc caggtgttag gcagcatgga
    22081 ctgccattgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg gcaatttcgc
    22141 cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc tgggatgtga
    22201 aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac ggagatcggg
    22261 aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac gtgtcccgag
    22321 cggtagccag atgcccgcca gcgtgggaac ccacagcccg agcgtcatca gcagcgtcaa
    22381 cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac ccagcatggg
    22441 tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg tggcatgttt
    22501 aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat ttttcaatat
    22561 cgccatagct ttcaattaaa tttgaaattt tgtaaaatat ttttagtaac ttaaatgtga
    22621 ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa acagtacgac
    22681 aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt ttttgacttt
    22741 ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg tattttcgta
    22801 aacgatgacg gcagccgccc ggtgctggaa gagctgaaaa agcacggcgt taaatatatc
    22861 gccctgcgct gtgccggttt caataacgtc gaccttgacg cggcaaaaga actggggctg
    22921 aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc catcggtatg
    22981 atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga cgctaacttc
    23041 tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt tatcggtacc
    23101 ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg
    23161 ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt cgatctgcca
    23221 accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc ggaaaactac
    23281 catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat cgtcaatacc
    23341 agtcgcggtg cattgattga ttctcaggca gcaattgaag cgctgaaaaa tcagaaaatt
    23401 ggttcgttgg gtatggacgt gtatgagaac gaacgcgatc tgttctttga agataaatcc
    23461 aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa cgtgctgttt
    23521 accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca gactacgctg
    23581 caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt ttaatcttgc
    23641 cgctcccctg caatccaggg gagctgattc agataatccc caatgacctt tcattctcta
    23701 ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag aaagtagccg
    23761 cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa attgctgtta
    23821 cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt aagcccgtga
    23881 cctgcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt tccggcagca
    23941 tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca gccaaagggc
    24001 tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat aacaccatta
    24061 gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta acgctggcga
    24121 ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag ctgccacagc
    24181 tcccggcggc aagtgactgt tcgctacagc gtttgccgtt gggtaatgca cacatcccaa
    24241 tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt gcacaatttg
    24301 cttctccact actggacatc gacgctttta aacctggcgc tacgtgcgcc gcagtcgcct
    24361 gctgaacagg ctcactacta cacgccgaca acaataaagc ggcacaccct acccaaaacg
    24421 ctgctcgcat ctcttcttcc tctgatcttc aagccaaacg acaccgccat aaataatagg
    24481 cagcacagag ggcgacgtcg agagctgtcc tgcgcgttgc cccgcatttt tactttttta
    24541 tggctatttt tttgccctct gtttgatcaa aacattcatt acgctgatgt gggggacaca
    24601 aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa agattactac tgggcgcgca
    24661 gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta ctattgacgg
    24721 taatggcgcg gttgcttcgg tggcgtttcg caccagtgaa gttatcgcca tctaccctat
    24781 tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg gcttgaagaa
    24841 cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg ctatcgctac
    24901 cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat cgcagggttt
    24961 gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctcacaccgt ttgtcctgca
    25021 tgtagcggca cgtaccgttg ccacacatgc actctctatt tttggcgatc attccgacgt
    25081 catggcggtg cgccagacgg gttgcgcgat gttgtgtgcg gcaaacgtcc aggaagcgca
    25141 agactttgct ctcatttcgc acatcgcgac gctgaaaagc cgcgtgccat ttattcattt
    25201 ctttgatggt ttccgtacgt cccacgaaat caataaaatt gtcccgctgg ccgatgacac
    25261 tattcttgat ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg cgctcaaccc
    25321 ggaacatccc gtgatccgcg gtacgtcagc caatcctgac acttatttcc agtctcgcga
    25381 agccaccaac ccatggtaca acgcggtcta tgaccatgtt gaacaggcga tgaatgattt
    25441 ctctgccgcg acaggtcgtc agtatcagcc gtttgaatat tacgggcatc cgcaagcgga
    25501 acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg ttgatgaatt
    25561 gctaacccgt ggcgaaaaag ttggcgtgct gaaagttcgc ctgtaccgtc ccttctccgc
    25621 caaacacttg ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac tggacagaac
    25681 caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtaatgaccg cactggcaga
    25741 agcctttaat aatggcgagc gcgaaactct gccccgtgtc attggtgggc gctatggtct
    25801 ttcatccaaa gaatttggcc cggactgtgt actggcggta tttgccgagc tcaacgcggc
    25861 taaaccgaaa gcgcgcttta cggttggtat ttacgatgat gtgaccaatc tgtcactgcc
    25921 gttgccggaa aacaccctgc caaactcggc gaaactggaa gccttgtttt atggccttgg
    25981 tagtgatggc agcgtttccg cgaccaaaaa caatatcaag attatcggta attccacgcc
    26041 gtggtacgca cagggctatt ttgtttacga ctccaaaaag gcgggcggcc tgacggtttc
    26101 tcaccttcgc gtgagcgaac agccgattcg ttccgcttat ctcatttccc aggctgattt
    26161 tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc gtttaaaacc
    26221 tggcggcatt ttcctgctca acacgccgta cagcacagat gaagtgtggt cgcgcttgcc
    26281 gcaggaagtt caggccgtgc taaatcagaa aaaagcgcgc ttctatgtga ttaacgcggc
    26341 gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc agatggcttt
    26401 tttccatctg acacaaattc tgcctggcga tagcgccctc gcagaattgc agggtgcgat
    26461 tgccaaaagt tacagtagca aaggtcagga tctggtggaa cgcaactggc aggctctggc
    26521 gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc acagcgccaa
    26581 tcgaccgcca gtggtttccg atgccgcccc tgctttcgtg aaaaccgtaa ccgctgcgat
    26641 gctcgccggg cttggtgatg ccctccccgt ttcggcgctg ccgccagacg gcacctggcc
    26701 gatgggcacc actcgctggg aaaaacgcaa tatcgccgaa gagatcccca tctggaaaga
    26761 ggaactctgt acccaatgta accactgcgt cgccgcttgc ccacactcag ctattcgcgc
    26821 aaaagtggta ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt cgctggatgt
    26881 gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggctccgg aagattgcac
    26941 cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc cagagattaa
    27001 agccatcaat atgatgtcgc gcctggaaca tgtcgaagaa gagaaaatca attacgattt
    27061 cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata ttcgcacatc
    27121 gcagctgatt acaccgctgt ttgaatattc cggtgcttgc tccggttgtg gcgagacgcc
    27181 gtatattaaa ttgctgactc agctctatgg cgaccggatg ttgatcgcta acgccaccgg
    27241 ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg atgccaacgg
    27301 tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg gccttggttt
    27361 ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc aatttgccga
    27421 taaaatcccg gcggaattac tgacggcgtt gaaatcagac gccacgccag aggttcgtcg
    27481 tgaacaggtt gcagctttac gccagcaact caacgatgtt gccgaagcac atgaactgct
    27541 gcgtgacgca gatgcactgg tggaaaaatc aatctggctg attggtggcg acggctgggc
    27601 ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa acgtcaacat
    27661 tctggtgctg gatacgcaat gctattccaa caccggtggt caggcgtcga aagcgacacc
    27721 gctgggtgca gtaactaaat ttggcgagca cggcaaacgt aaagcgcgta aagatcttgg
    27781 cgtcagtatg atgatgtacg gtcatgttta tgtggctcag atttctctcg gcgcgcagct
    27841 gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccgggaccat cgctgatcat
    27901 tgcttatagc ccgtgtgaag agcatggtta cgatctggca ctcagccatg accagatgcg
    27961 ccaactcaca gctaccggct tctggccgct atatcgcttc gatccgcgtc gtgccgatga
    28021 aggcaaactg ccgctggcgc tggattcacg cccgccgtca gaagcactgg aagaaacgtt
    28081 acttcacgag caacgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg cagaacagtt
    28141 atggaaagat gctgcagctg atttgcaaaa acgctatgac ttcctggcac aaatggccgg
    28201 aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc aattctaaaa
    28261 aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca gtggcaatgc
    28321 aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc gcagaataac
    28381 gtatagaaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat acattcacca
    28441 gacttattat catttcagag gaattatttg attaaggttt tacttaaggc gtaacaaatg
    28501 atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga aatggggata
    28561 aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gcaaagtact ggcactttta
    28621 attcctgccc tgctcggcgc aggtgctgca catgcagccg aagtttataa taaagacggc
    28681 aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga taattcagcg
    28741 aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca aattaacgat
    28801 caacttactg gctacggtca atgggaatac aatattcagg caaacaacac tgaatcttca
    28861 aaaaaccagt catggacccg tctggcattt gccggactga aattttcaga ttacggttct
    28921 ttcgattacg gacgtaatta tgggctggac cgatatgctg cctgaatttg gcggtgactc
    28981 ttataccaat gcagacaact ttatgactgg tcgtgccaat ggcgtcgcga cttatcgtaa
    29041 tactgatttc ttcggtctgg taaatggtct gaacttcgcg gtgcagtatc aaggtaacaa
    29101 cgaaggtgcc agtaatggtc aggaaggcac caacaacgga cgtgatgttc gccatgaaaa
    29161 cggtgacggc tggggtcttt ccacaacata tgatttaggc atgggcttta gcgctggtgc
    29221 ggcatacacc tcttctgacc gcaccaatga ccaggttaac catactgcgg cgggtggtga
    29281 taaagcagac gcgtggactg ctgggctaaa atacgatgct aacaatattt acctggcaac
    29341 catgtattca gaaacgcgta atatgacccc gtttggcgac agcgattatg ctgttgcaaa
    29401 caaaacccag aactttgaag tcactgcaca gtaccagttt gattttggtc tgcgtccggc
    29461 agtctctttc ctgatgtcta aaggccgtga cctgcacgct gccggtggtg cagacaaccc
    29521 ggcaggtgtt gatgataaag atctggttaa atacgccgat gttggcgcga cttactattt
    29581 caataaaaac atgtccactt atgttgacta taaaatcaac ctgttggatg aagatgacag
    29641 tttctacgct gccaacggca tctctaccga tgatattgtc gctttaggtc tggtttatca
    29701 gttctaaatc ctcctgcccg ctgttatggc gggctttttc tgcttattct tcctcctttg
    29761 atctaaatta aaaatgtgaa ctccgtcatt acacaaaaag tgtcatctgg cgttacactt
    29821 tatgcggata ctaaaacagg aggttttatg aacagaacga ttcttgtccc tatcgatatt
    29881 tccgattcag aattaactca acgcgtgatt agccacgttg aggcagaggc aaagattgat
    29941 gatgcagagg ttcatttcct gacggtaata ccttcactgc cctactatgc ctctctgggt
    30001 ttagcgtatt ccgcagaatt accggcaatg gatgacctga aagcggaagc caaatcgcaa
    30061 ctggaagaga tcattaaaaa atttaaactg ccaaccgaca gagtgcatgt ccatgttgag
    30121 gaaggctcgc ccaaagaccg cattctggaa ttggcgaaga agatccccgc tcatatgatc
    30181 atcattgctt cccatcgacc ggatatcacc acttatctgc tcggttccaa cgccgcagct
    30241 gtagtgcgtc acgcagagtg ctccgtgctg gttgtgcgct gacactaacg cccgcacatt
    30301 gctgcgggct ttttgattca tttcgcaaat gtgctgacat tttcccctct aatccgtacc
    30361 atacacgcca cagtttttat atcagatttc ttatgctggg cgttccggca tgacgcatac
    30421 tcttctgatg ccatataacg aattgagtcg cttttaaatg tcgcaaaatc aagaaattag
    30481 caagaaagaa caatacaacc tgaacaagtc agagtaaaaa ccgcaactat ctgacgaaca
    30541 ataactttta tgtttttcaa tatgttaggc aggattaaca actacaataa agcacaataa
    30601 tctacacttt ttttgcccca tttttgcccc ttttgcggcc ttttgtccca tttttgccac
    30661 agtaaaaatc tataactaat caatctggcg cccatgattt ctttccaata gaatcagttc
    30721 cggctggaaa tcattagata ctcgcactat cgaaaagctt accagccaac cgcggcacgt
    30781 tcttgcatac gaggtgccgc gttttacatg ccgtaccgag aattaatttc attttacgaa
    30841 aagttaaccc gccattgcta ccttaatttt ttaaaacgaa gttacctctg tcag
//
LOCUS       DAZKIF010000050        30740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZKIF010000050 DAZKIF010000000
VERSION     DAZKIF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.050"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..159
                     /gene="tuf"
                     /locus_tag="ACHOTW_002219"
     CDS             <1..159
                     /gene="tuf"
                     /locus_tag="ACHOTW_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9844327.1"
                     /translation="ELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGA
                     GVVAKVLS"
     gene            389..772
                     /gene="secE"
                     /locus_tag="ACHOTW_002220"
     CDS             389..772
                     /gene="secE"
                     /locus_tag="ACHOTW_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9844328.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            774..1319
                     /gene="nusG"
                     /locus_tag="ACHOTW_002221"
     CDS             774..1319
                     /gene="nusG"
                     /locus_tag="ACHOTW_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9844329.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1478..1906
                     /gene="rplK"
                     /locus_tag="ACHOTW_002222"
     CDS             1478..1906
                     /gene="rplK"
                     /locus_tag="ACHOTW_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9844330.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1910..2614
                     /gene="rplA"
                     /locus_tag="ACHOTW_002223"
     CDS             1910..2614
                     /gene="rplA"
                     /locus_tag="ACHOTW_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9844331.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2906..3403
                     /gene="rplJ"
                     /locus_tag="ACHOTW_002224"
     CDS             2906..3403
                     /gene="rplJ"
                     /locus_tag="ACHOTW_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9844332.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3470..3835
                     /gene="rplL"
                     /locus_tag="ACHOTW_002225"
     CDS             3470..3835
                     /gene="rplL"
                     /locus_tag="ACHOTW_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9844333.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4155..8183
                     /gene="rpoB"
                     /locus_tag="ACHOTW_002226"
     CDS             4155..8183
                     /gene="rpoB"
                     /locus_tag="ACHOTW_002226"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9844334.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8260..12483
                     /gene="rpoC"
                     /locus_tag="ACHOTW_002227"
     CDS             8260..12483
                     /gene="rpoC"
                     /locus_tag="ACHOTW_002227"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9844335.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12696..13139
                     /locus_tag="ACHOTW_002228"
     CDS             12696..13139
                     /locus_tag="ACHOTW_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFP9844336.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(13574..14707)
                     /gene="thiH"
                     /locus_tag="ACHOTW_002229"
     CDS             complement(13574..14707)
                     /gene="thiH"
                     /locus_tag="ACHOTW_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP9844337.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14704..15474)
                     /gene="thiG"
                     /locus_tag="ACHOTW_002230"
     CDS             complement(14704..15474)
                     /gene="thiG"
                     /locus_tag="ACHOTW_002230"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP9844338.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15476..15676)
                     /gene="thiS"
                     /locus_tag="ACHOTW_002231"
     CDS             complement(15476..15676)
                     /gene="thiS"
                     /locus_tag="ACHOTW_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP9844339.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15660..16415)
                     /gene="thiF"
                     /locus_tag="ACHOTW_002232"
     CDS             complement(15660..16415)
                     /gene="thiF"
                     /locus_tag="ACHOTW_002232"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP9844340.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16408..17043)
                     /gene="thiE"
                     /locus_tag="ACHOTW_002233"
     CDS             complement(16408..17043)
                     /gene="thiE"
                     /locus_tag="ACHOTW_002233"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP9844341.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17043..18938)
                     /gene="thiC"
                     /locus_tag="ACHOTW_002234"
     CDS             complement(17043..18938)
                     /gene="thiC"
                     /locus_tag="ACHOTW_002234"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP9844342.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19019..19119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19171..19647)
                     /gene="rsd"
                     /locus_tag="ACHOTW_002235"
     CDS             complement(19171..19647)
                     /gene="rsd"
                     /locus_tag="ACHOTW_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP9844343.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19742..20515
                     /gene="nudC"
                     /locus_tag="ACHOTW_002236"
     CDS             19742..20515
                     /gene="nudC"
                     /locus_tag="ACHOTW_002236"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP9844344.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20555..21619
                     /gene="hemE"
                     /locus_tag="ACHOTW_002237"
     CDS             20555..21619
                     /gene="hemE"
                     /locus_tag="ACHOTW_002237"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP9844345.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21629..22300
                     /gene="nfi"
                     /locus_tag="ACHOTW_002238"
     CDS             21629..22300
                     /gene="nfi"
                     /locus_tag="ACHOTW_002238"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP9844346.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22343..22933
                     /gene="yjaG"
                     /locus_tag="ACHOTW_002239"
     CDS             22343..22933
                     /gene="yjaG"
                     /locus_tag="ACHOTW_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP9844347.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            23120..23392
                     /gene="hupA"
                     /locus_tag="ACHOTW_002240"
     CDS             23120..23392
                     /gene="hupA"
                     /locus_tag="ACHOTW_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP9844348.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23405..24100
                     /gene="yjaH"
                     /locus_tag="ACHOTW_002241"
     CDS             23405..24100
                     /gene="yjaH"
                     /locus_tag="ACHOTW_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP9844349.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHFLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24102..24527)
                     /gene="zraP"
                     /locus_tag="ACHOTW_002242"
     CDS             complement(24102..24527)
                     /gene="zraP"
                     /locus_tag="ACHOTW_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP9844350.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24765..26141
                     /gene="zraS"
                     /locus_tag="ACHOTW_002243"
     CDS             24765..26141
                     /gene="zraS"
                     /locus_tag="ACHOTW_002243"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP9844351.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            26138..27463
                     /gene="zraR"
                     /locus_tag="ACHOTW_002244"
     CDS             26138..27463
                     /gene="zraR"
                     /locus_tag="ACHOTW_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP9844352.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27460..28749)
                     /gene="purD"
                     /locus_tag="ACHOTW_002245"
     CDS             complement(27460..28749)
                     /gene="purD"
                     /locus_tag="ACHOTW_002245"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9844353.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28761..30350)
                     /gene="purH"
                     /locus_tag="ACHOTW_002246"
     CDS             complement(28761..30350)
                     /gene="purH"
                     /locus_tag="ACHOTW_002246"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9844354.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7214 a         7964 c         8419 g         7143 t
ORIGIN      
        1 gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca acatcaaaat ggttgttacc
       61 ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg caatccgtga aggcggccgt
      121 accgttggcg cgggcgttgt agcaaaagtt ctgagctaat tgccgataac atttgacgca
      181 atgcgcacta aaagggcatc atttgatgcc ctttttgcac gctttcgtac cagaacctgg
      241 ctcatcagtg attttctttg tcataatcat tgctgagaca ggctctgttg agggcgtata
      301 atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg cgatcgcggg gtgaaaatgt
      361 ttgtagaaaa cttctgacag gttggtttat gagtgcgaat accgaagctc aaggaagcgg
      421 gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca ttgctcttgg tggcgattgt
      481 cggcaactat ctttatcgcg acattatgct gccgctgcgt gcgctggccg tagtaattct
      541 gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa ggtaaagcta ccgttgcttt
      601 tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg ccgactcgcc aggaaacatt
      661 gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg tcactgatcc tgtggggact
      721 ggatggtatt ctggttcgcc tggtatcctt tatcactggc ctgaggttct gagatgtctg
      781 aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc cggttttgaa ggccgcgtag
      841 caacgtcgct gcgtgagcat atcaaattac acaacatgga agatttgttt ggtgaagtca
      901 tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca gcgtcgcaaa agcgaacgta
      961 aattcttccc tggctacgtc ctcgttcaga tggtgatgaa cgacgcgagc tggcacctgg
     1021 tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac ttccgatcgt cctgcgccaa
     1081 tcagcgataa agaagtcgat gcgattatga accgcctgca gcaggttggt gataagccgc
     1141 gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt taatgatggt ccgttcgctg
     1201 acttcaacgg tgttgttgaa gaagtggatt acgagaaatc tcgtctgaaa gtgtctgttt
     1261 ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag ccaggttgaa aaagcctaac
     1321 ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg cgcgagattg gaatacaatt
     1381 tcgcgccttt tgtttttatg ggtcttgccc gtaaaacgat tttttatatc acggggagcc
     1441 tctcagaggc gttattaccc aacttgagga atttataatg gctaagaaag tacaagccta
     1501 tgtcaagctg caggttgcag ctggtatggc taacccgagt ccgccagtag gtccggctct
     1561 gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc
     1621 catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt tacgctgacc gttctttcac
     1681 tttcgttacc aagactccgc cggcagcagt tctgctgaaa aaagcggctg gtatcaagtc
     1741 tggttccggt aagccgaaca aagacaaagt gggtaaaatt tcccgcgctc agctgcagga
     1801 aatcgcgcag accaaagctg ccgacatgac tggtgccgac attgaagcga tgactcgctc
     1861 catcgaaggt actgcacgtt ccatgggcct ggtagtggag gactaagaaa tggctaaact
     1921 gaccaagcgc atgcgtgtta tccgcgagaa agttgatgca accaaacagt acgacatcaa
     1981 cgaagctatc gctctgctga aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt
     2041 agctgttaac ctcggcatcg acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt
     2101 actgccgcac ggtactggcc gttccgttcg cgtagccgta tttacccagg gtgcaaacgc
     2161 tgaagctgct aaagctgcag gtgctgaact ggtaggtatg gaagatctgg ctgaccagat
     2221 caagaaaggc gaaatgaact ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt
     2281 tggccagctg ggccaggttc tgggtccgcg cggcctgatg ccaaacccga aagtgggtac
     2341 tgtaacgccg aacgttgctg aagcggttaa aaacgctaaa gctggccagg ttcgttaccg
     2401 taacgacaaa aacggcatca tccacaccac catcggtaaa gtggactttg acgctgacaa
     2461 actgaaagaa aacctggaag ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc
     2521 gaaaggcgtg tacatcaaga aagttagcat ctccaccacc atgggtgcag gtgttgcagt
     2581 tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt
     2641 ctacaatctt acccccacgt atgctaacga aagttagcag ctaaaaagat ttgttcgttg
     2701 gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga aacttaatcc
     2761 cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct tgtttctgct
     2821 caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag agattttctc
     2881 tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac aagcgattgt
     2941 tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg
     3001 cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat
     3061 gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct
     3121 gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc cgggcgctgc
     3181 tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc
     3241 ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc tgccgaccta
     3301 cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg gcaaactggt
     3361 tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt tatcttttta
     3421 acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa tgtctatcac
     3481 taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg tagaactgat
     3541 ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg tagctgctgg
     3601 cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag ctgctggcgc
     3661 taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc
     3721 taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca aagacgacgc
     3781 agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta aataagccaa
     3841 cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta gtcaccagcc
     3901 tttttgcgct gtaaggcacc agtagcgttt cacactgttt gactactgct gtgcctttca
     3961 atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct gtgtaaatcg
     4021 caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc attttccggt
     4081 caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga cttgtcagcg
     4141 agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt aaggattttg
     4201 gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt gactcgtttc
     4261 agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct ttccgttccg
     4321 tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc taccgccttg
     4381 gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc
     4441 tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc accgtaaaag
     4501 acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac aacggtacct
     4561 ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct
     4621 tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat aacgcgcgca
     4681 tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac aacctgttcg
     4741 tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgtgcc ctgaactaca
     4801 ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc cgtgataaca
     4861 agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatcc tttgacatcg
     4921 aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc
     4981 agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc gcaggtaaag
     5041 tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca gcgaacatgg
     5101 agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt atcgaaacgc
     5161 tgttcaccaa tgatctggat cacggcccgt atatctctga aaccttacgt gtcgacccaa
     5221 ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc
     5281 cgactcgtga agcagcggaa agcctgttcg agaacctgtt cttctccgaa gaccgttatg
     5341 acctgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa gaaatcgaag
     5401 gttctggtat cctgagcaaa gacgacatca ttgatgttat gaaaaagctc atcgatatcc
     5461 gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt atccgttccg
     5521 ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag cgtgcggtga
     5581 aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg atcaacgcca
     5641 agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct cagtttatgg
     5701 accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca ctcggcccag
     5761 gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg actcactacg
     5821 gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc aactctctgt
     5881 ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt aaagtgactg
     5941 acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc aactacgtta
     6001 tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac ctggtaacct
     6061 gccgtagcaa aggcgaatcc agcttgttca gccgtgacca ggttgactac atggacgtat
     6121 ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg
     6181 ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact ctgcgtgctg
     6241 ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg
     6301 cggttgctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag
     6361 ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac ctgaccaaat
     6421 acacccgttc taaccagaac acctgtatta accagatgcc gtgtgtgtct ctgggtgaac
     6481 cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt gaactggcgc
     6541 ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc gaagactcca
     6601 tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac attcaggaac
     6661 tggcgtgtgt gtcccgtgac accaagctgg ggccagaaga gatcaccgct gacatcccga
     6721 acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttat attggtgcgg
     6781 aagtgaccgg tggcgacatt ctggttggta aggttacgcc gaaaggtgaa actcagctga
     6841 ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcgtctgac gttaaagact
     6901 cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag gtctttactc
     6961 gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg
     7021 cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc agccgtatcc
     7081 gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaattg ccgcgcgatc
     7141 gctggctgga gctgggcctg accgacgaag agaaacaaaa tcagctggaa cagctggctg
     7201 agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa
     7261 tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta tatctggcgg
     7321 ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa
     7381 tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg ccggtagaca
     7441 tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc
     7501 acttgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg aaacagcagc
     7561 aggaagtcgc gaaactgcgt gaattcatcc agcgtgcgta cgatctgggc gctgacgttc
     7621 gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg gctgaaaacc
     7681 tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta
     7741 aagagctgct gaaacttggc gacctgccga cttctggtca gatccgcctg tacgacggcc
     7801 gcactggtga acagttcgaa cgtccggtaa ccgttggtta catgtacatg ctgaaactga
     7861 accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc ctggttactc
     7921 agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag atggaagtgt
     7981 gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcacc gttaagtctg
     8041 atgacgtgaa cggtcgtact aagatgtata aaaacatcgt ggacggcaac catcagatgg
     8101 agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca
     8161 acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc ggggtaaaac
     8221 ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt attaaagttt
     8281 ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct ggcttcgcca
     8341 gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat caactaccgt
     8401 acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc ggtaaaagat
     8461 tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag
     8521 tgtggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca catcgaactg
     8581 gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat cggcctgctg
     8641 ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta tgtggttatc
     8701 gaaggcggta tgaccaacct ggagcgtcag cagatcctga ctgaagagca gtatctggac
     8761 gcgctggaag agttcggtga cgaattcgac gcgaaaatgg gtgcggaagc aatccaggcc
     8821 ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa
     8881 accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct ggaagcgttc
     8941 gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt actgccgcca
     9001 gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga cctgaacgat
     9061 ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg
     9121 ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg
     9181 gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct gaaatctttg
     9241 gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt
     9301 gactactccg gtcgttctgt aatcaccgtg ggtccatacc tgcgtctgca tcagtgcggt
     9361 ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa gctggaactg
     9421 cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc
     9481 gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa ccgtgcaccg
     9541 actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg taaagctatc
     9601 cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga ccagatggct
     9661 gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc
     9721 aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta
     9781 ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg catggtgctg
     9841 actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct gcatgcgcgc
     9901 gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc
     9961 agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc gaaaggtctg
    10021 ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc
    10081 tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat catgtacacc
    10141 ggctttgcat atgcagcgcg ttctggtgca tctgttggta tcgatgacat ggtcatcccg
    10201 gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat tcaggagcag
    10261 ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga tatctgggct
    10321 gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga aaccgttatt
    10381 aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat gatggccgac
    10441 tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg
    10501 gcgaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg
    10561 aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc ggataccgca
    10621 ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg
    10681 gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc ggttatcgag
    10741 ggtggtgacg ttaaagagcc gctgcgcgat cgcgtattgg gtcgtgtaac tgctgaagac
    10801 gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct gcacgaacag
    10861 tggtgtgacc tgctggaaga gaactctgtc gacgcggtta aagtacgttc tgttgtatct
    10921 tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc gcgtggccac
    10981 atcatcaaca agggtgaagc aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt
    11041 acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa
    11101 tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg
    11161 aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact gatcgacgaa
    11221 ttcggtcgta ccaaagaaag ctacaaagta ccttacggtg cggtactggc aaaaggcgat
    11281 ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac catgccggtt
    11341 atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca gaccattact
    11401 cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc cgcagaacgt
    11461 accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac
    11521 gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt
    11581 cagctggaag atggcgtaca gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa
    11641 tccggcggta ccaaggacat caccggtggt ctgccacgcg ttgcggacct gttcgaagca
    11701 cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa
    11761 gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga tccgtacgaa
    11821 gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt
    11881 gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat
    11941 gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca gggcgttaag
    12001 attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt
    12061 aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg cgtcaagatc
    12121 gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg
    12181 ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc gttccaggag
    12241 accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg
    12301 aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc gtaccaccag
    12361 gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt gactgcagaa
    12421 gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc tgataacgag
    12481 taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atggggggag
    12541 tttagggaaa gagcatttgt cagaatattt aaggaatttc tgaatactca taatcaatgt
    12601 agagatgact aatatcctga aactgactga actaattgag tcaaactcgg caaggattcg
    12661 atactattcc tgtgtagctt tcttaaggaa cgagaatgaa acaggaagtg gagaagtggc
    12721 gaccttttaa acatccggat ggcgacgttc gtgatttatc gtttcttgat gctcatcagg
    12781 ttatctacgt tcagcatcat gaggacaagg agcctttaga gtatcgcttt tgggttacct
    12841 actctcttca ctgcttcaca aaagattatg aacatcagac gaacgaagaa aaactatcgt
    12901 taatgtacca cgcgcctaaa gaatctcgtc ccttctgcca gcaccgttat aacttagcgc
    12961 gcacacactt aaaaagaact attttggcgc tgccagaaag caacgttatt catgccgggt
    13021 atggtagcta tgccgtgatt gaggtggact tagacggagg agataaggca ttttactttg
    13081 ttgtgttcag ggctttcagt gaaatttttc accattgcct aaaacttatt gagaaataag
    13141 cagcttcctc agccctcaaa ataacaaaac ccaccttaag gtgggtttcg ccagagaatt
    13201 atctctggta ttcagaacgc cattaccgga ctttgccttg accttgcgat aatcgcaggt
    13261 tgcgggatgt ctgaatttct tcagtctgct gcatcctgga agatgagaac atgtgttctt
    13321 attttcgtct ctatcatagt tgagtattta ctctcttaca atcagatctc tttcattgct
    13381 caacaggcga tggcttcaga ctttgcatta cggaattttt aagaaaggca gggcgaaacg
    13441 aggaagaagc ttttcgccct ggacaacatt cctgctgacg gcactaccat aaaaaaacac
    13501 aacgctttca acaaccttgc gtttttcatc ttccgtagcg gctcggaata aattttcgtt
    13561 cttacattcc gtctcatagt ctttgcgagg cgcgtcccag atagctgtcc cagtctttcc
    13621 ataccggctg caaaccctga gcggttaacg cggcagcaac cgcttccggt ctgcgctcgt
    13681 cgtgcggtga gaactgttcc agctcggggt gattgtcggc atagccacct ggctgcgttt
    13741 tcgaaaaagc gctgacgtta ttaattgcca gcggaataac gcgatcgcga aaccacggtg
    13801 attcccgcgt ggagagtgac agttcaatct ccggtgcaag cagccggaag gcgcagatgg
    13861 tttgcactaa ctggcgttca tccataatcg acgcaggctc aatgccgcca gtacatggac
    13921 gcaggcgtgg gaaggagaca gagtagcggc tttgccagta atgctgttgc agccatagca
    13981 aatgttctgc aaccatatag cagtcaacgc gccagctgtc ggaaaggcca attagcgcgc
    14041 cgaggcctat cttatcaatc cccgcacggc ccagccgatc cggcgtttcc agccgccaga
    14101 agaagtcctg ttttttgcct ttcagatgat ggcgggcata agtcgcctcg tgatatgtct
    14161 cctgataaac catcacgcca tccagaccaa gttgctttaa ctcggcgtat tccgtctccg
    14221 ccagcggctg tacttccatc tgtagtgaag agaactgttc acgcagggca gggagatgac
    14281 gacgaaagta atccatcccc actttcgcct gatgttcacc ggtgactaaa agcagatgtt
    14341 caaagcccat ctcccgtata gcggcgcttt ccctggcaat atccgcttca tccagcgttt
    14401 tgcgcttgat gcggttgctc atagagaatc cgcagtacgt gcagtcgtta gcgcaaagat
    14461 tggaaagata aagcgggacg tagaaactaa ctgtgttgcc aaatcgctga cgggtcagac
    14521 gctgcgcccg ttgggccagt tgttccagat agccactggc ggctggcgat aacagcgcca
    14581 tcatatcgtc gcgggtgagt tgcgaggcat ttagcgcccg ctctacgtca gcagccgttt
    14641 tgccgttgat acgcaggcgg atgtcgtccc agtccagttg tcgccagcga tcgctgaagg
    14701 ttttcatacc gatgcctcca gaaatccggt cagcgggctg gtggcatgag caaaataact
    14761 gcggctgccc ggtccggact gaggtgccag caggcctgct tctactgcca gacgaaatgc
    14821 cttcgccatg ttgacgggat cgtccgcgac ggcaatcgcc gtattcacta acaccgcgtc
    14881 ggcccccatt tccagcgcct gcgcggcatg gctgggaacg ccgatgccag catcgacaac
    14941 caccggaact gtggcctgct ggataataat ctccagcatg gcgcgggttt ccagcccctg
    15001 attcgagcca atcggcgcgc cgagcggcat caccgctgca cagccgactt cttccagacg
    15061 tttacacaat accggatcgg ccccgcagta aggcagcacg acaaatccct gttgtaccag
    15121 cgtttcggcg gctttcaggg tttcgatggg atcgggcaac agccagcggg cgtcagggtg
    15181 aatctccaat tttaaccagt ttgtgcctaa cgcttcacga gccagatggg cggcgaaaat
    15241 ggcttcttcc gctgttttcg ccccggatgt atttggcagc agggtcacac ccgccgcgat
    15301 aagcggttcg aggatagcgt cgttgtgctg gcgcaagtcg acacgtttca tcgccagcgt
    15361 caccagctgg ctgccggaag cgcggattgc ctctaccatc agttgtgaag aggcgaattt
    15421 cccggtgccg gtaaacagat gtgaatcaaa cgttttgtcc gcaatacgta acatctcaac
    15481 cccctgcaat aacctgaaaa agtaggatct ggtcgccatc ctgcacgata tgttgcgtcc
    15541 actgctcacg cgggacgatt tgctgattaa tcgccagagc cgcgcccgct tgtcgttgaa
    15601 cgagttgctc cagtagttcg tgaacagttt gcccggcggc gcactgcatc ggttgatcgt
    15661 taaacaggat ctgcattgct tcctccgcat accgggcaac cactggcgcg gcgcaacgcc
    15721 aggctgcgcc actggctcga cttaccgtcg aacagtcgga gttctcccgc aggcgtctct
    15781 ataccgctta ataacttaat ggcttccagt gcctgcaaag tgcccataac cccgaccacc
    15841 gggccaacca cgcccgccgt gcggcagttg cgttctggct cctggttttc tggccacagg
    15901 cagcggtaac aaccctgccc ccagggcggc gtcagtacca tcaactgacc gccaaatccg
    15961 accgcgctgg cggtgataag cggcgtgttg agtgccacgc aggcggcatt aatctcctgg
    16021 cgagtcgcca tattgtcggt acagtcgagc accacatcgg cttgtgcaac cgcatctttt
    16081 aacgcctcac ccgttaaccg ttgttgtaat gctgtcagtt gaatatcggg attcaactgt
    16141 gtcagtcgct gatggctgac ctgcgatttc gggcgatcga tatcttcagt ggtaaagagg
    16201 atttgtcgtt gcagattgct taaatgcaca tcgtcgtcat ctgccagtac cagcgtcccg
    16261 acgccagcgc ccgccaggta tagcgcagca ggtgtaccca gcccgccaag accgataatc
    16321 agcacctggc tgtcgagcag tttttgctgc ccgtcaagag cgatatcgtc gagcaggatt
    16381 tggcggctat aacgcataaa atcacggtca ttcatcgcca actcctgcaa tttccagcaa
    16441 ctgtgccgtt gccaaacgcc agtctgcggc ttgagtaatg gcgctgacga cggcgatact
    16501 gccgacaccc gttgctatca ccgcaggcgc gcgtgccaga ctgataccgc caatcgccac
    16561 ggtgggataa tccgacagtc gctcaacatg ccgtgccagc tgttccagcc cctgcggtgc
    16621 agaaggcatc tgtttggttt gcgtcgggaa cacatgtccc agcgcgatat aagaggggcg
    16681 tgctgccagc gcgacgtcga tttccatatc gtcatgggtc gaaacgccca gccgcaggcc
    16741 tgccgcgcgg atggcattga gatcggtggc ttgcaaatct tcctgcccca aatggacgcc
    16801 atacgcctga tgcttgatcg cgagccgcca gtaatcgttg ataaacaatc gcgcgttata
    16861 gcggcggccc agcgcaattg ccgccacgac atcggcttcc acctcttcat cgcgccgatc
    16921 tttgatgcgt agctggagag tacgtacgcc tgcatccaac agacgttcga tccactgtac
    16981 gctgtccacc accgggtaca gtcctaaacg aaaaggtaca ggaggaaaat caggctgata
    17041 catcacgctt cctccttacg caggtagatt tctccgcctc tggcacggaa gttctccgac
    17101 atatccgcta ttcccatttc aatagtttgc gtggcggcgt aatcacgcac ttcctggctg
    17161 attttcatcg agcagaattt cggcccacac atggagcaaa aatgggcgac tttacctgac
    17221 tcttgcggca gggtttcatc gtgataagcg cgggcggtaa acgggtcgag ggccagatta
    17281 aactggtctt cccagcgaaa ttcgaagcgg gctttcgaca tggcgttatc gcgaatttgt
    17341 gcgcccggat gccctttcgc caggtcagcg gcgtgggcgg caatcttata ggtgataagc
    17401 ccctgcttaa catcttcttt attaggcaga cccagatgct cttttggcgt tacgtaacag
    17461 agcatcgcgc agccaaacca gccaatcatc gccgcaccaa tccccgacgt gaagtggtca
    17521 tagcccggcg caatatcggt agttagcggc cccagagtgt aaaacggcgc ttcgtggcag
    17581 tgctctaact cctcggtcat attgcggcgg atcatctgca tcggcacgtg gcctgggcct
    17641 tcaatcatca cctgcacatc atattcccag gcgatttttg tcagttcgcc cagcgtatgc
    17701 agctcggcaa actgcgcttc atcgttggcg tcctgaatag aaccggggcg cagaccgtcg
    17761 cccagcgaca gcgaaacgtc ataagcggca cagatttcac aaatttcgcg gaagtgttga
    17821 tagaggaaat tttcctgatg atgggagagg caccatttcg ccataatcga accgccgcgc
    17881 gagacgatac cggtcaggcg tttcgcggtc atcggcacat agcgcagcag cacccccgca
    17941 tggatagtga agtaatccac accttgctcg gcctgttcca gcagcgtgtc gcggaacgct
    18001 tcccaggtaa gatcttcggc gatcccatta accttctcca gcgcctggta gatcggcact
    18061 gtaccaatcg gcaccgggct gttacgcaaa atccactcgc gggtttcgtg aatatagcga
    18121 ccggtggaga gatccatcac cgtatccgct ccccagcgcg tggaccatac cagcttttcc
    18181 acttcttctt cgatggaaga ggtgaccgcc gagttgccga tattggcgtt aacttttacc
    18241 aggaaattgc gaccaataat catcggctcc gattccggat gattaatgtt ggccgggata
    18301 atcgcacgtc cggcagcaac ttcatcacgg acaaattccg cagtgatatt ttccggcaga
    18361 cgtgcgccaa agctcattcc cggatgctgg tggcgtaaaa cttcgctacg gatgcgctcg
    18421 cggcccatat tctcgcggat ggcgatgaat tccatttccg gtgtgatgat gccctggcgg
    18481 gcgtagtgca gttgtgtgac gcggcgtcct gctttggcgc gttttggcgt taatacgccg
    18541 ctaaaacgca gctcgtcgag gccatcatct gccagccgcg ctttagtgta atcggaactg
    18601 cgcacggtaa gttcttcggt atcgccgcgc gcatcgatcc acggctggcg tagttttgcc
    18661 agcccttgct gcacgttaat ggcgatctgc ggatcgccat acgggccgga ggtgtcgtag
    18721 accggaatcg cttcgttttc ttcgtactgc ggctgttctt tgctaccgcc aattagcgtc
    18781 gggctaagct ggatctcacg catcggcacg cgcacgccgg gttgtgtgcc agtgatataa
    18841 atgcgttttg agttgggaaa ggcggtgcct tccagggtgt cgataaaatg ttgggcctgg
    18901 gcgcgttgtt cgcgacgggt cagttttgtt gcagacatag ctcattccaa aaagttaagg
    18961 acgtggcttg tcagacgacg gatgaagcaa gagacgatcg ccgcaaaggc gatgcgatag
    19021 cagattaact cttgttccct tcgcaggtat tagcctgatc aggttccgcg gatcccgaat
    19081 aaacggtctc agccagttaa ggcactccga caagaaatta gccccacaaa cggggcgttg
    19141 aatgtaaatt acgcgttaac agcgcagaac tcaagcagga tgtttgacgc gggcggcgtc
    19201 aagcaccagc agaatcagct tatcttccag cacaaagcgc gcttccagcg cctcgccgat
    19261 gtcagataaa acctgttgaa actcaaggta attatcatga tcgatagcgg tttccaggct
    19321 ggaatcgtag taatccataa tctgttgggt attggcttcg agttgcggcc aaatcttcgc
    19381 ggcgcgcgcg agttgcccgt tgccttccag cttatgaaga atgcgctcat aaatactgaa
    19441 atgtccggca gacaagtaat cgaccaggct ctgacaaaaa tcatcaaggg ctttttcgtt
    19501 tagcctcatg tacgattctt tgccaggctt aatgccaacc agattgtagt aagccacgag
    19561 cagatgctta cgtacatgta gccagcgatc aaccagtttg ttacttcctc tgacgcgctc
    19621 cgtcaggtta tcgagctggt taagcatgat tgactccgca agtttgtatt caaaaactgc
    19681 tcagtgagaa atgtaaaaac catgttaaac atgccagtga tgcaaaggta gtgcaagagc
    19741 tatggatcgt ataattgaaa aattagatca cggctggtgg gtcgtcagcc atgaacaaaa
    19801 attatggttg ccgaagggag aattgccata tggcgaagcg gcaaatttcg atcttgtggg
    19861 tcagcgcgca ctacaaatcg gcgaatggca gggggaacct gtttggttaa tacaacagca
    19921 gcggcgttac gatatggggt cggtacgtca ggtcattgat ctcgatgttg ggctgtttca
    19981 actggccgga cgtggcgtac aactggcgga gttttaccga tcgcataaat actgtggtta
    20041 ctgcgggcat gaaatgtatc caagcaaaac cgaatgggcg atgctgtgca gccattgccg
    20101 tgagcgttac tacccgcaaa tcgccccctg cattattgtt gccatccgtc gcgatgattc
    20161 gatcctcctc gcccagcata cccgccatcg taacggtgtc catacggtac ttgccggatt
    20221 cgtcgaagtg ggcgaaaccc tcgagcaggc ggtcgcgcgg gaagtgatgg aagagagcgg
    20281 aattaaagtt aaacacttgc gttatgtgac ttcccagccg tggccgtttc ctcagtcttt
    20341 aatgaccgcg tttatggcgg aatatgacag cggcgacatc gtgatcgacc cgaaagaatt
    20401 gctcgaggcg aactggtatc gctatgacga tttgccgtta ctcccgccgc ccggcaccgt
    20461 agcgcgccgt ctgatagaag atacggtggc gatgtgtcgg gcagagtatg agtgatgata
    20521 cactgaccgc ctgacgcact aaggaacagc caaaatgacc gaacttaaaa acgatcgtta
    20581 tctgcgggcg ctgctgcgcc agcccgttga tgtcactcct gtatggatga tgcgccaggc
    20641 gggtcgctat ctaccggaat ataaagccac gcgcgcccag gcgggcgatt ttatgtcgct
    20701 gtgcaaaaac gccgagctgg cgtgtgaagt gaccttgcaa ccgctgcgtc gctacccgct
    20761 ggatgcggcg atcctctttt ccgatatcct caccgtcccg gacgcgatgg ggttagggct
    20821 ctattttgaa gccggagaag gtccgcgttt tacctcgcca gtcacctgca aagctgacgt
    20881 cgataaactg ccaattccgg acccggaaga tgaactgggg tacgtgatga acgcggtgcg
    20941 taccattcgt cgcgaactga aaggcgaagt gccgctgatt ggtttttccg gcagcccgtg
    21001 gacgctggcg acctacatgg tggaaggcgg cagcagcaaa gccttcaccg tgatcaaaaa
    21061 aatgatgtat gccgatccgc aggcgctgca cgctctactc gataaactgg cgaaaagcgt
    21121 cgctttgtat ctgaatgcgc agattaaagc cggtgctcag gctgtgatga ttttcgacac
    21181 ctggggcggc gtgcttaccg ggcgcgatta tcaacagttc tcgctttatt acatgcataa
    21241 aattgttgat ggtttactgc gtgaaaacga cggtcgccgc gtaccggtca cgctgtttac
    21301 caaaggcggc ggacagtggc tggaagccat ggcagaaacc ggttgcgatg cgctgggcct
    21361 cgactggaca acggacatcg ccgatgcgcg ccgccgtgtg ggcaataaag tcgcgttgca
    21421 gggtaatatg gatccgtcga tgctgtacgc gccgcctgcc cgcattgaag aagaagtagc
    21481 gactatactt gcaggtttcg gtcacggcga aggtcatgtc tttaaccttg gtcacggcat
    21541 tcatcaggat gtgccgccag aacatgctgg cgtgttcgtg gaggcagtgc atcgactgtc
    21601 tgaacagtat caccgctaag gagcgattat ggatctcgcg tcattacgcg ctcaacaaat
    21661 cgaactggct tcttctgtga tccgcgagga tcgactcgat aaggatccgc cggatctgat
    21721 cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct
    21781 gctgaaatac ccctcgcttg agctggtgga gtataaagtg gcccgcatcg ctaccaccat
    21841 gccttacatt ccaggttttc tttccttccg cgaatatcct gcgctgctgg cagcgtggga
    21901 gatgctgtca caaaagccgg atttagtgtt tgtcgatggt catgggatct cgcatcctcg
    21961 ccgtcttggc gtcgccagcc attttggttt gctggtggat gtgccgacca ttggcgtagc
    22021 gaaaaaacga ctatgcggta aattcgaacc gctctccagc gaaccgggcg cgctggcccc
    22081 actgatggat aaaggcgagc agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc
    22141 gctatttatc gctactggtc atcgggtcag cgtggacagc gcgctggcgt gggtacaacg
    22201 ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga
    22261 acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa
    22321 tttccgtatt tgagaactca acatgttaca aaacccaatt catctgcgtc tggaacgcct
    22381 ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc gaacgcatgt accccaatta
    22441 cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa atttaccgtc gtattctcga
    22501 tctcatctgg gaaacgctga ccgttaaaga tgcaaaagta aatttcgaca gccaactgga
    22561 gaaatttgaa gaagcgattc cttcagccga cgatttcgat ctgtacggcg tttatccggc
    22621 aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg cgtttgagtg gtgaaacgct
    22681 cgaacacgcg gtggaagtga gtaagacctc catcacgacc gttgcgatgc tggaaatgac
    22741 tcaggctggt cgcgaaatga gcgatgaaga gctcaaagaa aacccagctg tagagcaaga
    22801 atgggacatt cagtgggaaa tattccgact tttagctgag tgcgaagaac gcgatatcga
    22861 gctgataaaa ggccttaggg cagacctgcg tgaggcggat gagagcaata ttggtataat
    22921 ttttcagcaa taagaccaga aaacgtgatt taacgcctga tttgtcgtac ctggagtctt
    22981 ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa aaaagtggct atcggtgcgt
    23041 gtatgcagga gagtgctatt ctggcatttc cgtcgcactc gatgcttagc aagcgataaa
    23101 cacattgtaa ggataactta tgaacaagac tcaactgatt gatgtaattg cagagaaagc
    23161 agaactgtcc aaaacccagg ctaaagctgc tctggagtcc actctggctg caattactga
    23221 gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc ggtaccttca aagtgaacca
    23281 ccgcgctgag cgtactggcc gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc
    23341 taacgtaccg gcatttgttt ctggcaaggc actgaaagac gcagttaagt aagattgcgt
    23401 ggcagtgaac agttttaacg aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc
    23461 attgatgctg gctggcgctc tgtttctcac tgcttgtagt cacaactctt cacttcctcc
    23521 ctttaccgcc agtggatttg ctgaagacca gggcgcggta cgcatctggc gaaaagacag
    23581 cggcgataat gtgcattttc ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac
    23641 gcgagagtat cgctggcagg gcgataacct cacgctcatc aatatcaatg tttacagcaa
    23701 acccccggtg aatattcgcg cgcgttttga cgatcgcggt gatctgagct ttatgcaacg
    23761 tgaatccgat ggggaaaagc agcagctttc taacgaccaa atcgatttat accgttatcg
    23821 ggctgatcag atccgccaga ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca
    23881 ggggcgctgg catgcgatgg aacagaccgt gaccacctgc gaagggcaaa ccattaaacc
    23941 tgatttagat tcgcaggcaa tagcgcatat cgagcgccgc cagagccgct cttctgttga
    24001 tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa ttactgttag tggcaaactc
    24061 tgatttctgt cgctggcaac ccaacgagaa aacgttctga tttaccagtg gcccataccc
    24121 atatgaccgc caccaccgca gccgccgtag cccatgccca ttccggctcc gcgcggaata
    24181 cccgcttcag ccatcgcaat atcccgtttc acccgtaact catctaacga ctgacgcaaa
    24241 ttctccatct ctttggcgac cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac
    24301 agggcattgt attcataacg cttcgtcacc agttgctgtt gcagtgcgct gctttgagcg
    24361 taaaagtcat tatggatttt ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca
    24421 ttttgctgcc acataccgtg tccgccgtga gcaaatgcag atgtcgatcc catcgccatt
    24481 gctgaaagcg ccatcattac cagggcaatt ttcgtgttcc gtttcatggt taatcctcca
    24541 gtggttgtct tacttcgggt attgcatctt ccgtgccaac gatgaaacgc tgatatgacg
    24601 ggtaatctgg catgataaac gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt
    24661 ttactcattg aaacttaagc ctttgtgtta cagcgcaggg taagcgctga taaaagatgg
    24721 catgatttct gctgtcagaa agggatgagc aggcaaagaa gaagatgcgt tttatgcaac
    24781 gttctaaaga ctccttagct aaatggttaa gcgcgatcct ccccgtggtc attgttgggc
    24841 tggtagggtt gtttgcggtg acggtgattc gcgattatgg ccgcgagact gccgccgcca
    24901 gacaaacgct gctggaaaaa ggcagtgtac ttattcgtgc tctcgaatcc ggctcgcgcg
    24961 tcggcatggg gatgcgcatg catcatgcgc agcagcaggc cttactggaa gaaatggccg
    25021 ggcagcctgg agtacgttgg tttgcggtca cggatgaaca aggaacaatc gtgatgcata
    25081 gcaactccgg catggtggga aaacagcttt attccccgca ggaaatgcag cagttacatc
    25141 cgggagatga agaagtgtgg cggcggatcg atagcgcaga cggcgagcct gttctggaaa
    25201 tttatcgcca gtttcaaccg atgtttgctg ctggaatgca ccggatgcgc catatgcagc
    25261 agtatgccgc gacaccacaa gcaattttca tcgctttcga cgccagtaat attgtgagtg
    25321 ccgaagatcg tgagcagaga aacaccctga ttatcctctt cgccctggcg acggtcttgc
    25381 tggcaagcgt attgtcattc ttctggtatc gccgctatct gcgctcgcgc cagcttctac
    25441 aagatgaaat gaagcgcaaa gagaagctgg tggcgctggg gcatcttgcg gcaggcgttg
    25501 cccacgaaat tcgtaacccg ctttcctcga ttaaagggct ggcaaaatac tttgccgagc
    25561 gcgcgccagc agggggagaa gcgcatcaac tggcgcaggt gatggcgaaa gaagctgacc
    25621 gtttaaaccg cgtggtaagc gagctgctgg aactggttaa gccaacgcat ctggctttgc
    25681 aggcggtgga tctcaacacg ctgattaacc actcattaca gctggtaagc caggatgcaa
    25741 actgccggga gatccagtta cgctttaccg ccaacgacac gttgccggaa attcaggccg
    25801 atccggacag gctgactcag gtcctgttga atctttatct caatgctatt caggcgattg
    25861 gtcagcatgg cgtgattagc gtgacggcca gcgaaagcgg cgcgggcgtg aaaatcagcg
    25921 ttaccgacag cggtaaggga attgcggcag atcagcttga agccatcttc accccgtact
    25981 tcaccaccaa agccgaaggc accggactgg ggctggcggt cgtgcataat attgttgaac
    26041 aacacggtgg tacaattcag gtcgcaagcc tggagggaaa aggcgcaagg ttcaccctct
    26101 ggcttccggt caatattacg cgtaaggacc cacaaggatg acgcacgata atatcgatat
    26161 tctggtggtg gatgatgaca ttagccactg cactattttg caggctttgc tgcgcggctg
    26221 gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg ctggagcagg tgcgggagcg
    26281 ggtttttgat cttgtgcttt gcgatgtgcg aatggcggag atggacggca ttgccacgct
    26341 aaaagagatc aaagcgttaa atccggcgat tccggtactg attatgaccg catactctag
    26401 cgtcgagacg gcggtagagg cgttgaaaac cggggcgctg gattatctca tcaagccgct
    26461 ggatttcgat aacctgcagg cgacgctgga aaaggcgctc gcgcatacgc acattattga
    26521 tgctgaaacg cctgcggtga ctgccagcca gttcggtatg gtcggtaaaa gcccggcgat
    26581 gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg gaagccacgg tgctgatcca
    26641 cggcgattcc ggcaccggta aagagctggt cgccagggcg attcacgcca gtagcgcacg
    26701 tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc aacgaatcct tgctggaatc
    26761 tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc gataaacgcc gggaagggcg
    26821 ctttgttgag gcggacggcg gcacgttgtt tctcgatgaa attggcgata tctcgccgat
    26881 gatgctggtg cgtctgctgc gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa
    26941 ccagactatt tcggttgatg tccggctgat tgccgcgacc catcgcgatc ttgccgcaga
    27001 ggtgaatgcc gggcgttttc gccaggatct ctactatcgc ctgaacgtgg tggcgattga
    27061 agtaccatcg ctgcggcaac ggcgggaaga tattcctctg ctggctggcc attttctgca
    27121 gcgctttgct gaacgtaatc gcaaggcggt aaaaggcttt acgccccagg cgatggatct
    27181 gttgattcat tacgactggc cgggaaatat tcgtgaactg gaaaatgcgg tggaacgggc
    27241 ggtggtgctg ctgactgggg aatatatttc cgaacgcgag ctgccgctgg cgattgccag
    27301 tacgccgatc ccgctggcac aaagcctgga tattcagccg ctggtggaag tcgaaaaaga
    27361 agtgattctg gcggcactgg agaaaacggg cggcaacaaa accgaagccg cccgtcagtt
    27421 agggatcacg cgcaaaacgc tgttggcaaa attgtcgcgt tagttctgct cgcgttcgat
    27481 agcgcgccag ccgatatctt tccggcagaa gcagtcgtcc cagtggatat cggtcattaa
    27541 ggcataggcg cgtttctgtg cttctgctac ggtatgaccc agcgcggtga cgcacagtac
    27601 gcgcccgccg ctggttacca cctgctcgtc atccgccagt tttgtgcccg cgtggaacac
    27661 tttgccgtct tccacttctt ccagcggcag gccgtggatc acgtcaccgg tgcggtaatc
    27721 gcccggatat ccacccgcag ccatcaccac gccgagagaa gcacgttcat cccactctga
    27781 cgttttctcg tctagtttgc cttcacaggc cgccaggcag agttcaacaa gatcggactt
    27841 catgcgcagc ataatcggct gggtttctgg atcgccaaag cggcagttaa attcgataac
    27901 cttcggattg ccctgtttgt cgatcatcag gcccgcgtag agaaaaccgg tgtaggtgtt
    27961 gccttccgac gccatgcctt tcacggttgg ccagatgata cgttccatcg tgcgctgatg
    28021 aacgtcatct gtcactaccg gcgcaggaga gtaagcgccc attccgcccg tgttcggccc
    28081 ggtatcttta tcgcctacgc gtttgtgatc ctggctggtg gccatcggca gcacatgctc
    28141 gccgtccacc atcacgataa agctcgcttc ttcgccgtcg aggaactctt cgataacgat
    28201 gcgatgaccc gcgtcgccaa acgcgttgcc cgccagcata tcgtgaacag ccgcttccgc
    28261 ttcttccagc gtcatcgcca cgataacgcc tttcccggca gccagaccgt ccgctttaat
    28321 gacgattggc gcgcctttct cacgcagata cgccagcgca ggttctacct cggtgaagtt
    28381 ctgatattcc gccgtaggaa tgttatggcg ggccaggaaa tctttagtaa acgctttcga
    28441 gccttccagc tgtgcggcgc ctgcggttgg gccgaagatt ttcatcccgg cggcgcggaa
    28501 ggtatcgacc acgcctttca ccagcggcgc ttccgggccg acgatggtta gatctacctt
    28561 ttcgttttgc gcgaaatcca gcagcgccgg gatatcggtc acgccgatag cgacgttttg
    28621 cagcgtgggt tccagcgctg tgcctgcgtt acccggagca acaaaaacag tctcaaccag
    28681 cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc ccgccgttac caatcactaa
    28741 tactttcatc tgtcgctcca ttaatggcgg aagtggcgca tatcggtgaa gagcatcgca
    28801 ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac ggatggaacc gccaggctgg
    28861 attacgcagg tcacgcccgc agcggcggcg gcatcaatac cgtcgcggaa cgggaagaac
    28921 gcgtcagaag ccatcgagga acctttcact tccaggcctt catcggcagc tttaataccg
    28981 gcgattttcg cggagtacac gcggctcatc tggcccgcgc caataccgat ggtcatattg
    29041 tttttggcat agacgatagc attggatttc acgaacttcg ccactttcca gcagaacagc
    29101 gcatcacgca gttcctgttc ggtcggctga cgtttggtga cgacgcgcag ttcttccgca
    29161 ccgaccatgc ccagatcgcg atcctgaacc agcagaccgc cgttcacgcg tttgaaatca
    29221 agacccggaa cacgctcgcc ccactgaccg caggtcagaa cgcgtacatt ctgtttggcg
    29281 gcggtgattt tcagggcttc ttcgctggcg gacggcgcaa taatcacttc aacaaactga
    29341 cgagaaatga tggcctgtgc ggtttccgca tccagctcgc ggttaaaggc aatgatgccg
    29401 ccgaatgcgg aggtcgggtc ggttttgtac gcgcgatcgt aagcatcaag aattgaattg
    29461 ccgatagcca cgccgcaagg gttggcgtgc ttcacaatca cacatgccgg ctcggcgaac
    29521 tctttcacgc actccagcgc cgcatcggta tcggcgatgt tgttataaga gagggcttta
    29581 ccctgaacct gggttgcggt agcaacggag gcttctttca cattctcttc tatatagaag
    29641 gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct gcttcttaat gaagttcagg
    29701 ttcagcgtgc gtgggaagcg accggcggct tctttgcttt caccgtgata agccggaacc
    29761 atgctgccga agtagttggc aatcatgctg tcgtaggcgg cagtgtgttc gaaggctttg
    29821 atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt cgttgtcatc catctcttta
    29881 ataatggcgt cgtagtcgct gctcttcacc acgattgcga catctttatg gttcttggcg
    29941 gcggagcgca ccatcgttgg gccgccgata tcgatgttct caaccgcatc ttccagcgag
    30001 caaccttcac gggccacggt ctgggcgaac ggatacaggt taacaaccac catatcgata
    30061 ggctggatct gatgttcttc cataatggca tcgtcctggc cgcgacggcc cagaatgcca
    30121 ccatgtactt tcggatgcag ggtcttcacg cgtccatcca tcatctccgg gaaaccggtg
    30181 taatcggaaa cttcggttac cggcagacct ttttctgcta acagacgggc agtgccccct
    30241 gtagacagca gctccacacc gcgtgcggaa agtgcctggg cgaattcgac gataccggct
    30301 ttgtcagaaa cactgagcag agcgcggcgg actggacgac gttgttgcat ggtaaatccc
    30361 ctggatttga ctattacaga gagcgttagc tgaatttttc gcgaaaaact cagctaacgc
    30421 ccctaacggg gcatccttat ttttcgcccg cattgtaacg aaaacgtttg cgcaacgctc
    30481 gcgaattttt ttaattcgat ggcgatcacg attttgcccc cgattagcgt gggagaaata
    30541 gagaaataac gctgatgatt gtggataact ctgtgcgtaa aaaggtataa agcggggttt
    30601 tgctggggaa tgcagcagtc agtcattttt ctgcaatttt tctattgcgg cctgcggaga
    30661 actccctata atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca caaacagccg
    30721 gttcggttga agagaaaaat
//
LOCUS       DAZKIF010000051        30048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZKIF010000051 DAZKIF010000000
VERSION     DAZKIF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.051"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            170..1753
                     /gene="yegH"
                     /locus_tag="ACHOTW_002247"
     CDS             170..1753
                     /gene="yegH"
                     /locus_tag="ACHOTW_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9844355.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1828..2166
                     /locus_tag="ACHOTW_002248"
     CDS             1828..2166
                     /locus_tag="ACHOTW_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9844356.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2156..2446
                     /locus_tag="ACHOTW_002249"
     CDS             2156..2446
                     /locus_tag="ACHOTW_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9844357.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2499..4352)
                     /gene="asmA"
                     /locus_tag="ACHOTW_002250"
     CDS             complement(2499..4352)
                     /gene="asmA"
                     /locus_tag="ACHOTW_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP9844358.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4374..4955)
                     /gene="dcd"
                     /locus_tag="ACHOTW_002251"
     CDS             complement(4374..4955)
                     /gene="dcd"
                     /locus_tag="ACHOTW_002251"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP9844359.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5047..5688)
                     /gene="udk"
                     /locus_tag="ACHOTW_002252"
     CDS             complement(5047..5688)
                     /gene="udk"
                     /locus_tag="ACHOTW_002252"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9844360.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            6006..9324
                     /gene="dgcE"
                     /locus_tag="ACHOTW_002253"
                     /pseudo
     CDS             6006..9324
                     /gene="dgcE"
                     /locus_tag="ACHOTW_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9425..10273)
                     /gene="alkA"
                     /locus_tag="ACHOTW_002254"
     CDS             complement(9425..10273)
                     /gene="alkA"
                     /locus_tag="ACHOTW_002254"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP9844361.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10407..11759
                     /gene="yegD"
                     /locus_tag="ACHOTW_002255"
     CDS             10407..11759
                     /gene="yegD"
                     /locus_tag="ACHOTW_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9844362.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11772..13712)
                     /gene="yegI"
                     /locus_tag="ACHOTW_002256"
     CDS             complement(11772..13712)
                     /gene="yegI"
                     /locus_tag="ACHOTW_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFP9844363.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13709..14470)
                     /gene="pphC"
                     /locus_tag="ACHOTW_002257"
     CDS             complement(13709..14470)
                     /gene="pphC"
                     /locus_tag="ACHOTW_002257"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFP9844364.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            complement(14467..15126)
                     /gene="yegL"
                     /locus_tag="ACHOTW_002258"
     CDS             complement(14467..15126)
                     /gene="yegL"
                     /locus_tag="ACHOTW_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9844365.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15347..15406)
                     /gene="ibsA"
                     /locus_tag="ACHOTW_002259"
     CDS             complement(15347..15406)
                     /gene="ibsA"
                     /locus_tag="ACHOTW_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP9844366.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15678..15734)
                     /locus_tag="ACHOTW_002260"
     CDS             complement(15678..15734)
                     /locus_tag="ACHOTW_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9844367.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(16006..16062)
                     /locus_tag="ACHOTW_002261"
     CDS             complement(16006..16062)
                     /locus_tag="ACHOTW_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9844368.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16342..17589
                     /gene="mdtA"
                     /locus_tag="ACHOTW_002262"
     CDS             16342..17589
                     /gene="mdtA"
                     /locus_tag="ACHOTW_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP9844369.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17589..20711
                     /gene="mdtB"
                     /locus_tag="ACHOTW_002263"
     CDS             17589..20711
                     /gene="mdtB"
                     /locus_tag="ACHOTW_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP9844370.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20712..23789
                     /gene="mdtC"
                     /locus_tag="ACHOTW_002264"
     CDS             20712..23789
                     /gene="mdtC"
                     /locus_tag="ACHOTW_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP9844371.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            23790..25205
                     /gene="mdtD"
                     /locus_tag="ACHOTW_002265"
     CDS             23790..25205
                     /gene="mdtD"
                     /locus_tag="ACHOTW_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9844372.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25202..26605
                     /gene="baeS"
                     /locus_tag="ACHOTW_002266"
     CDS             25202..26605
                     /gene="baeS"
                     /locus_tag="ACHOTW_002266"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP9844373.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26602..27324
                     /gene="baeR"
                     /locus_tag="ACHOTW_002267"
     CDS             26602..27324
                     /gene="baeR"
                     /locus_tag="ACHOTW_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP9844374.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27515..27847
                     /gene="yegP"
                     /locus_tag="ACHOTW_002268"
     CDS             27515..27847
                     /gene="yegP"
                     /locus_tag="ACHOTW_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP9844375.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27995..29356
                     /gene="yegQ"
                     /locus_tag="ACHOTW_002269"
     CDS             27995..29356
                     /gene="yegQ"
                     /locus_tag="ACHOTW_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP9844376.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29686..30003)
                     /gene="yegR"
                     /locus_tag="ACHOTW_002270"
     CDS             complement(29686..30003)
                     /gene="yegR"
                     /locus_tag="ACHOTW_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP9844377.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6956 a         8013 c         7908 g         7171 t
ORIGIN      
        1 atttcttaaa taatgtttta ttattaccac ttttaattca gggataatgt gaggttatta
       61 ccctcaaata atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca
      121 tgatcgaatt gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat
      181 tgccgatccg tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg
      241 cattgataac ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga
      301 ccgcgcacgg gttaccgggc tgttgctggc gatgttaatg cgcctgttac tgctggcgtc
      361 aatctcctgg ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag
      421 cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga
      481 gctgaacgaa cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa
      541 gttctggggg gtggtgacac aaattgtggt actggacgcc atcttctcac tcgactcagt
      601 gattaccgcc gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc
      661 tatcagcctg atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac
      721 catcgttatt ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg
      781 tttcggcttc gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat
      841 cgaggcgctc aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaaccagac
      901 actgcgccag cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaggatgc
      961 ggaactggac gccgaaaccg cgtccatgct ggtggatcat ggtaaccagc agatctttaa
     1021 tccgcaggaa cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtcagcag
     1081 cattatgacg tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagacgagat
     1141 ccgccagttg ctggagcgaa atcagcatac gcggctggtg gttactgatg gcgatgacgc
     1201 ggaagatttg ctcggtgttg ttcacgttat cgacctgtta caacaatcac tgcgcggcga
     1261 accgctcaac ctgcgggtgt taattcgcca gccgctggtg ttcccggaaa ccttgccgtt
     1321 gttacctgcc ctggagcagt tccgtaatgc ccgcacacac tttgcttttg tggtggatga
     1381 gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa
     1441 cttaccgaac gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg
     1501 ttcctggacg gcgaatggtc atatgccgct ggaagatctg gtgcaatatg tgccgctgcc
     1561 gctggatgaa aaacgtgaat atcacaccat tgccgggcta ttgatggaat atttgcagcg
     1621 tattccaaaa cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt
     1681 ggaaagccat cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga
     1741 gtacgaggtg tgataaaaca tcaaatgcgc aggttcaaac tgcgcatttg atttatacaa
     1801 aatttagtat aatctaagga tggagtaatg agaaaaaagc tggcttttct tgataccagt
     1861 cttgacgatt tacgcgcatt tccagaatcg tctcgacagg aaattggcta tcaactcgac
     1921 aggatccaac aagggttaaa cccatacgac tggaaacctt tttcaactat cggccctggt
     1981 gtgcgagaga ttcgtactcg cgatgctgat gggatctatc gcgtcatgta tgtcgcaaaa
     2041 tttgaagaag cggtttacgt attgcattgt tttcaaaaga aaacacagac taccagtcaa
     2101 tcggatattg atttagccaa acgacgctat aaagaacttg ttcaggagcg gaaaaatgaa
     2161 aattgaaacc tttgatagtg tgtgggatgc ggtgagtgac acccctgaac aagcggaaaa
     2221 tatgcggatc cgtgccgagc ttgtaacgat cattaataac tggattgaac aacaagggtt
     2281 tagccaggct caggcagctt ctgctttagg ggtcactcaa cctcgcatct ctgaacttgc
     2341 cagaggtaaa atccagatct ttagtatcga taaattgatc accatgatgg ctcatgcagg
     2401 actacatatt caacgtattg aagttcaata tccacatgct gcttagtgct ataaatacag
     2461 atggcgacaa attgatgccg ccatctatat aacttgtctt acatcttctc cagcaacttc
     2521 ttcacatctt tgccattgcg ggaatcttta ttccgctcgg cccagtcatt caggcgacgt
     2581 ttcgcttcgt cctgtagatg tttgcgcagt aactgatcca cttgcagact gtaattgagt
     2641 tgctgccagt tgccataaac ccgcagcggt actggcgttt ctttgaggaa atcaatcagt
     2701 ttgctttccc cgttccagcc acccacgacc cgaatatcaa actgggtgtc gcaggtttga
     2761 tctgccagat tcaacatgcc ttcccctgta agcgccagca caggcgattg accttgcatg
     2821 tcgtttaacg tcacgacgcc atccttcaac gtcaaatcgg tggtaaagcg gtcaagacgc
     2881 gttacgttat cgaagttttc agcggccttc acatcaccac cattacgttc taccgcttgc
     2941 tgaatcatct gctggaagtt catcccttcc atgcgcgtat cggccatttc gacatgtgcc
     3001 tgtccttgcc agttgtggcg gaatgcgtcg gcatctatgt cagcaccgga gaaatcacca
     3061 gccaatgaca tttttccggt caacgaaatc ggatagttaa acgccttcag aatggtgcca
     3121 atctcaacgt tttccagccg tggctggaag tttatccgcg gatttattga tgtcgcgtcc
     3181 agcgtgcccg gcagtgaaac ctgtccaccg ttaagcttgc cctgtagttg agtaatttcc
     3241 agcaaacccg acttgttggt catttgcgtg gcaacatctg taaaattcat tccgcgccag
     3301 cgcacgttac tggcctgcaa caaaatatca gccgtaaagc cttgcagtcc ctgataggcc
     3361 ggttcatcaa tacgcgaaga aataaccggg cgcggcaacg ttgattggct ctgcccctgc
     3421 tgcgcggcac cgttttcacc attcgcggtt tcattaagcg ggatgaggtt gtcaagattc
     3481 agttgcggga attgcagcct cagctgccat tccggtttct ctgtcagcgt gacctgtgct
     3541 tgcccgctca gcgtactatc attggcggtc aaactaattt ggttaaatga aaggcgttta
     3601 cgtgactcct gccactgggc ctggaaactc ccctgcccct gaataccttg ttttggtaaa
     3661 tcggcaccct gcaactgcca gttaatttgt tcaatagccg ccgttaaatc atgcggataa
     3721 tcagaagcat ctaccgtacc gttaagggat atcgtgagat cgcgctgatc gcgattaacc
     3781 cgcccggaga actcaaatga gccacgatgt tggggatctt gttccatttg caggcggata
     3841 ttgcggattg tcacctgctc gtcatcttca tgctggaaca ccagcacgct gtccgccacc
     3901 ttaagactgg atatatcaaa cgaccatccg cgatcgtctg acagatccgg caaggtattg
     3961 tcgcgcggtg caaccggagc gtcttcactg cgcaccgctt ccgtctgcgg cgtcagttgg
     4021 atcactgccc cttttagcat cacctgctta acgctcagtt gatgactcag tagtggtaaa
     4081 agcgccacgt ccaggcgcat gttgtcggcg cgaaccagtg gctggctcgc gccctgggcg
     4141 gtgagagaca ttcgcccgga gaggatacta agctgcggcc agacgtgcca acgcagtggc
     4201 ccgtcgagct gcaattgata accgctacgt gcagcaactt gcttgaccat atagtcgcgg
     4261 aaatcattcg gattcaccag caacactaac gcagataacc cggccaccag cacgaccagg
     4321 agtatcatca gcgtcgtcag aaatcgtctc atggtatcct caatgggcct gaattagtct
     4381 ttatcgattc ggctggctac cgcgccctgc tggttgcgat acttcgcatc ttcacggcgg
     4441 ttgtaaggtc gcgccgccgg gccggaaagc ggctcaaagc tcagcgcacc aattaacatg
     4501 cccggacgca gcgccagcgg cagcttaccg gagttgtaga actccagcac aatgcaacca
     4561 gaccagcccg gatcgatacg gtgcgcggtg acgtgcacca tcagccccag acgcgccagt
     4621 gaggaacgcc cgtccagcca gcccaccaga tcggctggca gcgtcaccga ctccagcgtc
     4681 accgccagcg ccagctctcc tgggtgaaga tagaacgcct cgctttcatc gagaacgatc
     4741 tcatcgctca tcacgcggtc aagcgcggcg ctcacttcat ctttgggacc gctcagatcg
     4801 ataaacgctg ccgtgtgacc acggaaggta cgaaatttat tgcccaggcg tacatccacc
     4861 gtcgcgccgt taatacgctc cactggcgga cgtgggttga tcgacaaacg gccttcatca
     4921 agccaggctt caatatctcg gtcacacaga cgcatggcat tttctccttt cgcgcatcac
     4981 tcccttaacg ccaattacgt caagggcata ccaggttatc actgaacggt acacaattta
     5041 tcaagcttat tcaaagaact gacttatttt cgctttcaat atatcgatcg cgatgcggtt
     5101 tttcccgccg cgcggcacga taatgtccgc atattgttta gaaggctcaa tgaattgcag
     5161 gaacatcggg cgcacggttt tttgatattg cgccatcacg gaatccattg aacgcccacg
     5221 ctcgttaacg tcacgcttga tgcggcgcat caggcagata tccagcgggg tatcaacgaa
     5281 aatggagaag ttaagttcgt cacgcaaacg tgcatccgtc agcaacaaaa tgccttcgag
     5341 aatgatgacc ttcttcggct caaccgtcac cgtttctttc atacgcgtat gttcaacata
     5401 gctgtaaacc ggcaggtcaa ttgccgagcc gcgtttcaac gcttgtaaat gctcaagcag
     5461 caggctgtga tccatcgcgc tgggatggtc gtagttggtc ttaacgcgtt cttccatcga
     5521 cagatggctt tgatctttgt aatagcagtc ttcgggaatt acgccgatgt gttcatcacc
     5581 gacttgctca cgtaattcac gataaagggt actggcaata agactcttgc cggaagccga
     5641 tgcgccagcg ataccgataa tgacgcactg atgagactga tcagtcatat atttagcgac
     5701 ctgattaacc tggatgttag gaagggggcg acgaagcgcc aaacgcggca attataggga
     5761 tttcatccgc ctgataccag tcgaatagcg ttgccgcgcg ctcagagtta attgttgacg
     5821 aagaattccc ggtggcaaat tacgttgatc agttttatac aaggtaaaaa ttgttatacg
     5881 cagttgcgca aattatccgc ctttacgtca ctttatgagc aattcgcata taaaatgtaa
     5941 aacttttgta ctagcataaa cacagaaacg aatactggcg acctggcctt gcggataaag
     6001 cggtaatgag caaacaatca cagcatgtat taattgccct gccccacccg ctgcttcacc
     6061 tggtcagttt aggtttagtc tcgtttatct tcaccctttt ctcgcttgag ctttcgcagt
     6121 ttggcaccca actcgcccca ctgtggttcc cgacgtccat catgatggtg gcgttttatc
     6181 gccatgccgg gcgcatgtgg ccagggattg cgctgagctg ctcgctggga aatatcgccg
     6241 catccatcct gcttttttcc accagctcgc tgaacatgac ctgcacgacc atcaatattg
     6301 ttgaagccgt ggtcggggca gtgctgctac gtaaattgct gccgtggtat aaccccttgc
     6361 aaaatctggc tgactggctg cgtctggcac tcggcagcgc cattgttcca cctctgttgg
     6421 ggggtgttct ggttgtcctg ctgacgcccg gagacgatcc tcatcagggc atttttgata
     6481 tgggtactgt cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa
     6541 ccacactatc tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta
     6601 gccatcacac tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc
     6661 attattgtgc tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc
     6721 ttccttacca cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct
     6781 acgccgcgta cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg
     6841 cccgccaaca tcatgacgat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc
     6901 gaaagcgaaa cccgttttcg gaacgccatg gaatattccg ccatcggcat ggcattagtg
     6961 ggcaccgagg gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt
     7021 caggaagagc tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa
     7081 gatctccaac aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa
     7141 cgttactaca accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc tctggtgcgc
     7201 cacacggatg gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa
     7261 cgcaccgaac aggtgaatca gcaactgatg gaacgcatca cgctggctaa cgaagcgggc
     7321 gggattggca tctgggagtg ggaactgaag ccgaatattt ttagctggga taagcggatg
     7381 ttcgagctgt atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc
     7441 gtgctgccgg aagatcgcca acacgccgaa aaagtgattc gtgattcgtt gcaatcacgc
     7501 tcgcccttta aactggaatt tcgcattgcc gtgaaagatg gcatccgcca tatccgcgcc
     7561 ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac gcctgctcgg cattaatatg
     7621 gatatgactg aggttaaaca gcttaacgag gcattgtttc aggaaaaaga gcgcctgcac
     7681 attacgctgg actccatcgg cgaagccgtg gtctgtattg atatggcgat gaaaattacc
     7741 tttatgaatc cagtggcgga gaagatgagc ggctggacgc aggaagaagc gttaggtgtt
     7801 ccgctcctga cggtgttgca tattactttt ggcgacaacg gaccattaat ggagaacatt
     7861 tacagtgccg acacctcacg ttccgcgatc gaacaagatg tggtgttgca ctgtcggagc
     7921 ggcggcagct acgacgtgca ttacagtatt acgccgttaa gtactctgga cggcagcaat
     7981 ataggctcgg tactggtgat tcaggacgtt accgaatcac gcaaaatgct gcgccagctg
     8041 agctacagcg cctcccatga tgcactgacg catctcgcca accgcgccag ttttgagaaa
     8101 caactgcgta tcctgctgca aacggtaaac agtacgcatc agcgacatgc actggtgttt
     8161 atcgatcttg atcgctttaa agcggtgaat gacagcgccg ggcatgcggc gggcgacgct
     8221 ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc gctccagtga cgtgctggcg
     8281 cggctcggcg gtgatgaatt tggtctgctg ctgccagact gcaatgttga aagcgctcgt
     8341 tttatcgcta cacgtattat cagtgctgtg aatgactatc actttatatg ggaaggccgg
     8401 gtacatcggg taggtgccag cgccgggatt accttgatcg atgacaacaa tcatcaggcg
     8461 gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg tggccggggc
     8521 cgggtgacgg tttacgaacc gcagcaagct gccgcacata gcgaacgggc ggcgatgtcg
     8581 cttgatgaac agtggcggat gattaaagag aatcagttga tgatgctcgc ccacggtgtc
     8641 gcttcgccac ggatcccgga agcgcgtaat ttgtggctga tttcacttaa gctctggagt
     8701 tgcgaaggcg agattattga tgaacaaaca tttcgtcgta gcttcagcga tccggcgctt
     8761 agccatgctc ttgaccgccg ggtattccac gaatttctcc agcaggccgc aaaagcggtt
     8821 gccagtaaag gcataagcat cgccctcccc ctttccgttg ccggtttgag tagcgccacg
     8881 ctggtgaatg atctgcttga gcagctggaa aatagccctc taccaccacg gttattacat
     8941 ctgattattc cggcagaagc gattttcgat cacgcagaaa gcgtgcaaaa actgcggctg
     9001 gggggatgcc ggatcgtatt cagtcaggtg ggccgcgatc tgcaaatctt caactcgctg
     9061 aaagcgaata tggcagatta cctgctactt gatggtgagt tatgcgccaa cgtgcagggt
     9121 aatttgatgg atgagatgct gattacgatt attcaggggc acgctcagcg actcgggatg
     9181 aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg atacgctttc tggcatcggc
     9241 gtcgatctga tttatggtga ggtgattgcc gatgcccaac cgctggattt gctgatgaat
     9301 agtagttatt tcgcgattaa ctgagggagc gtttgcccat tgcctgatgc gacgctaacg
     9361 cgtcttatca ggcctacaaa tcgctcattc cgcaaggcgc tcgcaccgca tccggcgacc
     9421 aacgtcatgc ttcgtctggt tgccagcctt ccgtatacca gatatgcaac agcgcataag
     9481 aacgccaggg cttccagcgc tcggcataac ggcggatttg cgccggtgtc attcccggaa
     9541 atcgctgttt aatcagataa tcatccggca ggaaaacatc tttcgcctgc cagccacgca
     9601 aagcaaaata attcgccgtc cagcgcccga tacccggaaa agtttgcagc gttttcatcg
     9661 cctgctccac atcgcccggt attgtcattg gtaaggtgcc ctccagcgcc gcatttgcca
     9721 gatgaatcag cgcctctgcc cgtttcaacg gcatacctaa cgcttttaat gcctgcgggt
     9781 ctgctgctgc cagccgctga ggcgtgggga agcagatata ctccggaaaa tcatccagcc
     9841 gttcgccata aagctgtacc actttggcag tcaattttgc cgccatcgcc acgctcacca
     9901 gttggcctaa aatcgcccgc acgccttgct caaaagcatc aacactgccg ggtaaacgca
     9961 atccgggccg cgccgcgcct aacttgccca acgcaccgtt aacaatctgc gggttacatt
    10021 gcagatcaag caggcggctc attttcgcca gacactcagc ggcaacgggt tctaaatctg
    10081 cacttaaatt tatgtgcaga gtatggcggg ctatatccgg aatagcagtc accacgccgc
    10141 gatattcgcc caccgccaga ctacgggcat aataatcgtc agcgaccgtt tccacaccac
    10201 tcactgcacg ggcggcgaga aatcccaaca tccacgacca gtcatacggc ggctgccagt
    10261 tcagggtata catcgcatct ccttattcat ccgctttcag cataaacgtt attcagacgc
    10321 tgcgctttgc tttcatattc cggttgtcgc gacggcaaca tttcgctaaa gtcacgcccc
    10381 ttcttcaccg gcatggggat tatttcgtgt ttattggttt tgattacggt acagcaaact
    10441 gttcagtagc ggtcatgcgt gacggtaaac cgcacttgct aaaaatggaa aacgacagca
    10501 cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaagc ggtaagcgaa tggctgtacc
    10561 gccatcatga tgttccggca gacgacgatg aaacgcaggc actgttgcgt cgggcgattc
    10621 gttataatcg cgaagaagat atcgatgtta cggcgaaaag cgtgcagttc ggtctttcct
    10681 cactggcaca gtacattgat gatccagaag aagtgtggtt tgtgaaatca ccaaaatcgt
    10741 tcctcggtgc cagcggctta aaaccgcagc aggtagcgct gtttgaggat ctggtctgcg
    10801 caatgatgtt gcacattcgc cagcaggcgc aagcgcagct gccagaagcg attactcagg
    10861 cggtgattgg tcgcccgatc aacttccagg ggctgggcgg cgatgaagca aacgcccagg
    10921 cgcaagggat tctggaacgc gcggcgaagc gtgccggatt cagggacgtg gtattccagt
    10981 acgagccggt agcggctggg ctggattacg aagccacctt acaggaagaa aaacgggtac
    11041 tggtggtgga tatcggcggt ggtacgactg actgttcatt gctgctgatg gggccgcagt
    11101 ggcgttcgcg tctcgatcgt gaagccagcc tgctgggtca cagtggttgc cgtattggcg
    11161 gtaacgatct ggatatcgca ctggcgttta aaaacctgat gccactgctg ggtatgggtg
    11221 gcgaaaccga aaaaggcatc gccctgccga ttctgccgtg gtggaatgcg gttgccatca
    11281 acgacgtacc tgcgcagagt gatttctaca gtagtgccaa cggtcgtctg cttaacgatc
    11341 tggtacgcga tgcccgcgaa ccggaaaaag tggccctgct acagaaagtc tggcgtcagc
    11401 gtttaagcta tcgcctggtg cgtagcgcag aagagtgcaa aattgcgctt tcaagcgtag
    11461 cggaaacccg cgcctcactg ccgtttatca gcgatgaact ggctacgctg attagccagc
    11521 aagggctgga aagcgccctc agccagccac tggcgcggat tcaggaacag gtgcaactgg
    11581 cgctggataa cgcccaggaa aaaccggacg ttatctacct gaccggcgga agcgcccgtt
    11641 ctccgctgat taaaaaagcg ctggcagaac agttgccggg cattccgatt gcaggcggcg
    11701 atgactttgg ctccgtcacc gccgggctgg cacgctgggc ggaagtggtg tttcgttaat
    11761 tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct gagccagttt ttctgccgcc
    11821 tgacgtaacg gttccatcaa cggcatggtc cgtgctggag catgaaggcg gaatcgctgc
    11881 aactctgtcg cgccgacagt caacgccgat tccagccgat gtcgtttggc ggccattttc
    11941 gccattaccg cttgtctttc tgcaggcgtt accgcttcgt tcggcctgaa cacaaaacgg
    12001 cgttcacagc tcgctttcca gtcgataacc gcctgggtca gatgatcacc aaacccttta
    12061 acttgcttaa ccccacgacg ggtaacatcc gcggctgttt caataccaaa agaccgtaac
    12121 gccgctttac gcgcagggcc aacgccggga atagaggcag catcaataaa aaatccctcc
    12181 agaaacttct gcttctgccg ttcccttgca gtgtcatgaa gtgctgccag agcgcgtttc
    12241 tcctcttccg gtaacccgag aatttcgtcc ttcatttttt cgagcatcgc ccgtttggcg
    12301 ataaaacctt ccagcccgcc caactgttgg atctggctga ctaaatggtc atagtccatt
    12361 ttcgcgcgat taaatgcctc acggcgttgc tggatttctg ctttataaac tttgcttgtc
    12421 agactgccaa taatccagat agccgccagc acaggaacca aaataatata acgcggttct
    12481 gcctgaaggc cgcaaagcaa cagcgataac gctgacagtg cgatctcaat cagaatgatg
    12541 tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag tcggttggaa atgatcgggt
    12601 aatggcaatt gcaatgctgg cggtgctact gacgccatca ccatcgccca gactttcgcc
    12661 agcacaaaat caccactggt ggtaatgacc tcttcgccga gatcaataaa atagataacg
    12721 ccttgattat ccagcgcaca ccacgggcag tcagtcaaat gaccggggta aacatgcatt
    12781 gccgaaacgg tacatttctt taactgttgg cgtagtaaat ccagtgccgc tacccacgct
    12841 tttgccgtcg gacgcgcggt tgccacgcca ctttccgtga atgcctgctg aaacatggct
    12901 tcaacatcgc ccggtaacat cgacagcgga atcgatcgtg gcggcggttt taaaccacgt
    12961 cgctggttgt ctgacgcgta ggcataacgg aaatgggcaa tatccgtctc cagcgcatta
    13021 cccgcatcag agataagcgg cacaccagaa taaggatgcc gcccaccaaa caagacgtga
    13081 aaaatcagca acgcaaggcc aaaattatcg tgattcgcgg tacgttcaaa accgacaaat
    13141 gatggcaagg tttgtagctc tggcggcgta aaatgcgaca cgccgacttc gcataaatgc
    13201 agtgtgccat tggcgttaat ctgaaaggag tcactatcga tcaacaccac tttgctgtcg
    13261 cgacctacca taaagctgtt ctggtttacg tcccccacga cgtgcccgtg ctcgtgaacc
    13321 gtagcaaaag atgaagcaat attgcgcgca acatagagta gaaaatccca cgcacaatga
    13381 ggataactct ggcgacgatg tgccgggcta tagatcatat gaatcggttc tttaccagaa
    13441 acttttggca tcataaaacc gataactttt ccgcctcgtc caccgtgaag cgttgcctgc
    13501 ggccaggcga cataattcaa caactgcgcg tcagctgtcg cagccataaa ggcaagtttg
    13561 tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg cgacgctatc gacaaactca
    13621 ttgatatcat aaaccgcgcc ttcgccacct ttgcccagtt cacggcctgg cgtaacgcac
    13681 tcaccagtag cagtatataa agtgggtttc atggtaacca cagcgccagt gtcaacgttt
    13741 tatcatcatc ggtacgctcg tttaccgctg ggctcgacaa aaactgcttt aacaattcag
    13801 gtaataaatc gagttgttcc tgcgttgctg ccgccagtcc attgaaaaac ggggtaaaga
    13861 aaggcacatg cggagaatta tccagcatat ttaacgccag tcgctgaata ccatccgtaa
    13921 atgctgcaac tttatgcgca cgctcagtgc tggtgaaagt ttccagtctg gaaacgacat
    13981 cttcatcggt aatgaaatgc gtcatattgg catattcacc caccatcggc gtaagcggta
    14041 attgcagacc gtgaccaaaa tccaccacca cgccgccatc gccaatctgc ataatcaagg
    14101 tgccgttagc cgatgaaatc aatcccagaa acgtgcaggc aaaatcgcga actgctaatt
    14161 ctttagcttc cgcctcggca aaaagtcgct gacgaacggt cagcacaata tccgtggcga
    14221 gaatatcatt gagtccaaat tcaccgtgct gcactttttg cgacacataa gccatcgctt
    14281 cattgatggc aagcatcgcg ccttcgccac cctgcgagac actgcctgcg ccatcagcaa
    14341 ggaacatcac cagtaatggc tgctggtcat ttagccaggc aacctgcatc tggcaggcat
    14401 cctgacaagg taaatcagcg ctaatgtgcg acgttccaac agcggatgca taaaccagac
    14461 gccagctcac actgacgtcc agccttttgg cgcttccagc acaacttccg ttcccggcgt
    14521 ggagcgggaa accgaacgca gcgaactgga taaccagcta aacagttcac ggaattgtaa
    14581 tccctgcagt ggcaaaggct gacgaacgct gatttgtgcc agagtcttaa tatccgcccc
    14641 ctgaacaccg atggaaaaga aggcaaactt cttatcttct tccccctgaa acactttgtt
    14701 ggcggcagcc tgccactcat cggttggtgc accatcggtg atcaggaaaa tccacgggcg
    14761 ataatacgaa ataccatttg cacgatactc acgtttccgc tcctctacca tatccagggc
    14821 tttggtaatg gcggcaccca ttggtgtatc cccctgggca aacaggatgg gcgggaaaaa
    14881 attagcggcg ctggtaaagg gttgttccac atgcaccggg ccgaacgtca caataccaag
    14941 ttccactctt tttaaggcca gcgggtcggc aagcagctca tcgcgaaagg taaccaatcc
    15001 ggcgttaagt tcattaatgg gtctaccgtt catcgaacca gaaacatcca gcagcaaaat
    15061 gcaggggcaa cgtggttccg ggttactggc aaaatcgctg gtggcaaacg tgatttgttc
    15121 gctcataaga aatcctttct tagagaatta ttgaggtccg gtatcatacc gattgcaata
    15181 atgaaataag agcaaacgaa gaaactttca gaattaggcc cggcggcgca gaaaattttg
    15241 ctattcgcct cgcaagaacg gagagccgtc gattgacact aagggcggag tgacataatt
    15301 tcaggagtga gggttaggga aaggtttccc cctccccctg gtgttcttag taagcctgga
    15361 agctaatcac taagagtatc accagtatga tgacgtgctt catcataacc cttttcttat
    15421 ttaaggcccc ttcctcggga ggggctttcc cgtttcagca tcccgctgaa atcgtcggct
    15481 tacctccttt cgccatgcat gcagtctatc gctgacttgc ggataaaata gtttccagtg
    15541 tgattattgc gtaacgtctc gcaaagaata tttgctggca ggatcgcaga ctgcaaagcc
    15601 tgcagattga caatcttatc gtgaaggcat actttcagga gtgagggtag agcggggttt
    15661 cccccgccct ggtagtctta gtaagccggg aagcttatga ctaagagcac cacgatgatg
    15721 atccacttca tcatgaccct ttccttattt aaggcccctt cctcgggagg ggctttcccg
    15781 tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg ccctgaattc agtctatcgc
    15841 tgacttgcgg ataaaatagt ttcctctgtg attattgcgt aacgtctcgc aaagaatatt
    15901 tgctggcagg atcgcagact gcaaagcctg cggattgaca ttctgaacgt gaaggcatac
    15961 tttcaggagt gagggtagag cggggtttcc cccgccctgg tagtcttagt atgctgggta
    16021 gctaatcact aagagcacca cgatgatgag tatcttcatc atgacccttt ccttatttat
    16081 ggccccttcc tcgggagggg gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttac
    16141 ctcctttcgc catgcaagca gtctatcgct aacgcgtaga taaaatagtt tcctgtgtta
    16201 ttactggatg cgtgctcgca aatgtgcccg ccattcagac gattcccgat agtgtttcat
    16261 aattcctcca tttttctccc ttattggctg gctacactag tatcattccg cgaaacgttt
    16321 caggaagaga aactcttaac gatgaaaggc agttataaat cccgttgggt aatcgtaatc
    16381 gtggtagtta tcgccgccat cgccgcattc tggttctggc aaggccgcaa tgactcccag
    16441 agtgcagccc caggggcgac gaaacaagcg cagcaatcgc cagcgggtgg tcgccgtggt
    16501 atgcgttccg gcccattagc cccggttcag gcggcgaccg ccgtagaaca ggcagttccg
    16561 cgttacctca ccgggcttgg caccattacc gctgctaata ctgttacggt gcgtagccgc
    16621 gtggacggtc aactgatggc gttacatttc caggaaggcc agcaggtcaa agctggcgat
    16681 ttactggcag aaattgaccc cagccagttc aaagttgcat tagcacaagc ccagggccaa
    16741 ctggcaaaag ataaagccac gcttaccaac gcccgccgtg atctggcgcg ttatcagcaa
    16801 ctggcaaaaa ccaatctcgt ctcccgccag gagctggatg cccaacaggc gctggtcagt
    16861 gaaaccgaag gcaccattaa ggctgatgaa gcaagcgtcg ccagcgcgca gctgcaactc
    16921 gactggagcc gtattaccgc accagtcgat ggtcgcgttg gtctcaagca ggttgatgtt
    16981 ggtaaccaaa tctccagtgg tgataccacc gggatcgtgg tgatcaccca gacgcatcct
    17041 atcgatttgc tctttaccct gccggaaagc gatatcgcta ccgtcgtgca ggcgcaaaaa
    17101 gccggaaaac cgctggtggt agaagcctgg gatcgcacca actcgaagaa attaagtgaa
    17161 ggcacgctgt taagtctcga taaccaaatc gatgccacta ccggtacgat taaagtgaaa
    17221 gcacgcttta ataatcagga tgatgcgctg tttcccaatc agtttgttaa cgcgcgcatg
    17281 ttagtcgaca ccgaacaaaa cgccgtagtg atcccaacag ccgccctgca aatgggcaat
    17341 gaaggccatt ttgtctgggt gctgaatagc gaaaacaagg tcagcaaaca tctggtgacg
    17401 ccgggcattc aggacagtca gaaagtggtg atccgcgcag gtatttctgc gggcgatcgc
    17461 gtggtgacag acggcattga tcgcctgacc gaaggggcga aagtggaagt ggtggaagcc
    17521 cagagcgcca ccactccgga agagaaagcc accagccgcg aatacgcgaa aaaaggagct
    17581 cgctcctgat gcaagtgtta cccccgagca gcacaggcgg cccgtcgcgc ctgtttatta
    17641 tgcgtcctgt ggccaccacg ctgctgatgg tggcgatctt actcgccggg attatcggtt
    17701 atcgcgccct gcccgtttcg gcgctgccgg aagtggacta tccgaccatt caggtggtca
    17761 cgctctaccc aggtgcgagc ccggatgtca tgacctctgc cgttaccgcg ccgctcgaac
    17821 gccagttcgg gcagatgtct ggcctgaaac agatgtcgtc gcaaagttcc ggcggtgcgt
    17881 cagttatcac tttacagttc cagctaacat taccgctgga tgtcgccgag caggaagtgc
    17941 aggccgcgat taacgctgcg accaacttgt tgccgagcga tctgcctaac ccgccggttt
    18001 acagcaaagt gaacccggca gatccgccga tcatgacgct cgccgtcacc tcaacagcca
    18061 tgccgatgac gcaagtggaa gatatggtgg aaacccgcgt cgcgcagaaa atctcgcaga
    18121 tttccggcgt cggcctggtg acgctttccg gcggccagcg tccggctgtt cgcgtcaaac
    18181 ttaacgctca ggcgattgcc gccctcggcc tgaccagcga aaccgtgcgc accgccatta
    18241 ccggcgctaa cgttaactcg gctaaaggta gccttgacgg cccttcccgt gcggttacgc
    18301 tttccgctaa cgaccagatg caatccgccg aagagtatcg ccagctaatc atcgcctacc
    18361 agaacggcgc gccgattcgt ctgggcgatg tcgcaactgt agagcaaggt gcagaaaaca
    18421 gctggctcgg cgcatgggcg aataaagaac aggccattgt gatgaatgtt cagcgccagc
    18481 ccggtgctaa cattatctcc accgccgaca gcatccggca gatgctgcca cagctcaccg
    18541 agagtctgcc gaaatcggtg aaggtgacgg tactttctga ccgtaccacc aatatccgcg
    18601 catccgtcga tgatactcag tttgaattga tgatggctat cgcgctggta gtcatgatta
    18661 tctacctgtt tttgcgcaat attccggcga ccatcattcc cggcgttgca gtgccgctgt
    18721 cgttaatcgg caccttcgcg gtcatggtgt ttctcgattt ttcaatcaat aacctgacgc
    18781 tgatggcgtt aactatcgcc accgggttcg tggtcgatga cgccatcgtg gtgatcgaaa
    18841 atatttcccg ctatatcgaa aaaggcgaaa aaccgttggc ggcggcgctc aaaggcgcag
    18901 gtgaaatcgg ctttaccatt atctcgctga ccttctcgct gattgcggtg ttgatcccac
    18961 tgctgtttat gggcgatatc gtcgggcgac tgttccgcga atttgctatt accctggcgg
    19021 tagcgatttt aatctcagcc gtggtgtcgc taaccctgac accgatgatg tgcgcacgga
    19081 tgctcagcca ggagtcgttg cgtaaacaga accgcttctc ccgtgcctcg gaaaaaatgt
    19141 tcgacaggat aatcgccgcc tatggtcgtg gactggcgaa agtgctgaat catccgtggc
    19201 tgaccttaag cgtggcgctc agcacgctgc tgcttagcgt tctattgtgg gtgttcattc
    19261 cgaaaggttt cttcccggta caggataacg gcattattca gggcactttg caggcaccgc
    19321 aatccagctc ctttgccaat atggcccagc ggcaacgcca ggtcgcggac gtgattttgc
    19381 aggatccggc agtgcaaagt ctgacctcat ttgttggcgt tgatggcact aacccgtcgc
    19441 tgaacagtgc gcgtttgcag atcaacctca aaccgttgga tgaacgtgat gatcgggtgc
    19501 aaaaagtcat cgcccgtctg caaacggcgg tggataaagt gccgggcgtc gatctcttcc
    19561 tgcaaccaac gcaggacctg accatcgata ctcaggtcag ccgcacccag taccagttta
    19621 ccttgcaggc cacgtcactg gatgcgctca gtacctgggt gccacagttg atggaaaaac
    19681 tccagcagct gccacagatt tctgatgtct ccagcgactg gcaggacaaa gggctggtgg
    19741 cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg gatcagcatg gcggatgtcg
    19801 ataacgccct gtacaacgcg tttggtcagc gactgatttc cactatttat actcaggcta
    19861 accagtatcg tgtagtgctg gagcacaata ccgaaaatac cccaggcctc gcggcgctgg
    19921 ataccattcg cctgaccagc agcgacggcg gcgtggtgcc gctaagctca attgccaaaa
    19981 ttgagcagcg ttttgcgccg ctttccatca accatctgga tcagttcccg gtaacgacca
    20041 tctcctttaa cgtgccggat aactattcgc tgggcgatgc ggtgcaggca attatggaca
    20101 ccgagaagac gctgaatctg ccggtggata tcaccacgca attccagggc agcaccctcg
    20161 ccttccagtc ggcgctgggc agcactgtct ggctgattgt cgcggcggtg gtggcgatgt
    20221 atatcgtgct cggcattctg tacgagagct ttattcaccc gatcaccatt ctctcgacgc
    20281 tacccacggc aggtgttggc gccctgctgg cgttaatgat tgccggtagc gaactggatg
    20341 tgattgcgat tatcggcatt attttgctga tcggtatcgt gaagaagaac gccatcatga
    20401 tgatcgactt cgcgctggct gctgagcgcg agcaaggcat gtcgccgcgc gatgcaatct
    20461 accaggcttg tctgttgcgt tttcgtccga tcctgatgac caccctggcg gctctgcttg
    20521 gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga actacgtcgt ccgttaggta
    20581 tcggcatggt cggcggcctg attgtcagcc aggtgctgac gctgttcacc acgccggtga
    20641 tttatctgct gtttgaccgc ctggcattgt ggaccaaaag tcgctttgct cgccatgaag
    20701 aggaggcgta agtgaagttt tttgccctct tcatttaccg cccggtggcg acgattttac
    20761 tgtcggtcgc cattaccctg tgcggcatac tgggtttccg tatgctgccg gtcgccccgc
    20821 tgccgcaggt cgattttccg gtgattatgg tcagcgcctc gctgcccggt gcgtcgccgg
    20881 agacaatggc gtcttccgtt gccacgccgc tggagcgctc acttgggcgc attgccgggg
    20941 tcagtgaaat gacctccagc agttcgctcg gcagcacgcg tattattttg cagtttgatt
    21001 ttgaccggga tatcaacggt gcggcgcgtg atgtgcaggc ggcgatcaac gccgcacaaa
    21061 gtctgctgcc cagtgggatg cccagccgcc cgacttatcg caaagcgaac ccgtcggatg
    21121 cgccaattat gatcctcacg ctaacctccg atacttattc gcagggtgaa ctgtacgatt
    21181 tcgcctcgac gcagctggct ccgacgattt cgcaaatcga cggtgtcggt gatgtcgatg
    21241 tcggcggcag ctcgctgccc gccgtgcgcg tcgggctgaa cccgcaggcg ctgtttaatc
    21301 agggcgtgtc gctggacgac gtacgcactg ccatcagcaa tgccaacgtg cgtaaaccgc
    21361 agggggcgct ggaagatggc actcaccgct ggcagatcca gaccaacgat gaactgaaaa
    21421 ccgccgctga atatcagccg ctgattattc actacaacaa cggcggcgcg gttcgtctgg
    21481 gcgatgtggc gacggtgacc gactcagtgc aggatgtgcg caacgccggg atgaccaacg
    21541 ccaaaccggc tattttactg atgatccgca aattgccgga agccaatatt atccagacgg
    21601 tagacagcat ccgggcaaaa ttaccggagt tgcaggaaac cattccggcg gcgattgatc
    21661 tgcaaattgc ccaggatcgc tcccccacca ttcgcgcctc gctggaagaa gtcgagcaaa
    21721 cgctgattat ctcggtggcg ctggtgattc tggtggtctt tttattcctg cgctcgggtc
    21781 gcgccactat tattcccgcc gttgcggtgc cggtttcgct gattggtacg tttgcggcga
    21841 tgtacctgtg cggattcagt ctcaataacc tttcgttaat ggcgctcacc atcgctactg
    21901 gtttcgtggt ggatgacgcc atcgtggtgc tggaaaacat tgcacgtcat ctggaagcgg
    21961 gaatgaaacc gttgcaagcc gcactgcaag gtactcgcga agtcggtttt acggtgctgt
    22021 cgatgagtct gtcgctggtg gcggtgttcc tgccgctgct gttgatgggc ggattgccgg
    22081 gccgactgtt acgcgagttt gccgtgacgc tttctgtcgc cattggtatt tcgttgctgg
    22141 tttctctgac attaacgcca atgatgtgtg gctggatgct gaaagccagc aagccgcgcg
    22201 agcaaaagcg actgcgtggt tttggtcgca tgttggtagc cctgcaacaa ggctatggta
    22261 agtcactgaa atgggtgctc aatcataccc gtctggtggg catggtgctg cttggcacca
    22321 ttgctttgaa tatctggcta tatatctcga ttccgaaaac cttcttcccg gagcaggaca
    22381 ctggcgtgtt gatgggcggg atacaggcgg atcagagtat ttcgtttcag gcgatgcgcg
    22441 gtaagttgca ggatttcatg aaaattatcc gtgacgatcc ggcagtggat aatgtcaccg
    22501 gctttaccgg cggttcgcga gtgaacagcg ggatgatgtt tatcaccctc aagccacgcg
    22561 acgaacgcag cgaaacagcg cagcaaatta ttgaccgtct acgcgtgaag ctggcgaaag
    22621 aaccgggggc gaatctgttc ctgatggcgg tacaggatat tcgcgttggc ggacgtcagt
    22681 cgaacgccag ctaccagtac acgttgttat ccgacgacct ggcggcactg cgcgaatggg
    22741 agccgaaaat ccgcaaaaaa ctggcgacat tgccggaact ggcggacgtg aactccgatc
    22801 agcaggataa cggcgcggag atgaatctgg tttacgatcg cgacaccatg gcacggctgg
    22861 gaatcgacgt acaggccgcc aacagcctgt taaataacgc cttcggtcag cggcaaatct
    22921 cgaccattta ccagccgatg aaccagtata aagtggtgat ggaagtggat ccgcgctata
    22981 cccaggacat cagcgcgctg gaaaaaatgt tcgtaatcaa taacgaagga aaagcgatcc
    23041 cgctgtcgta tttcgctaaa tggcaaccgg cgaatgcccc actatcggtg aatcatcagg
    23101 gattatcggc agcctcgacc atctcgttca acctgccgac cggaaaatcg ctctcggacg
    23161 ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt gccttcgacg gtgcgcggca
    23221 gttttgccgg cacggcgcag gtgttccagg agacgatgaa ctcacaggtg atcctgatta
    23281 tcgccgccat cgccacggtg tatatcgtgc tgggtatcct ttacgagagt tacgtacatc
    23341 cgctgacgat tctctccacc ctgccctcgg cgggcgttgg agcgctgctg gcgctggagc
    23401 tgttcaatgc cccgttcagc ctaatcgccc tgatagggat catgctatta atcggcattg
    23461 tgaagaaaaa cgccattatg atggtcgatt ttgcgcttga agcccaacgg cacggtaacc
    23521 tgacgccgca ggaagctatt ttccaggcct gtctgctgcg ttttcgcccg attatgatga
    23581 ctaccctggc ggcgctgttt ggcgcgctgc cgctggtgtt gtcgggcggt gacggctcgg
    23641 agctgcggca acccttgggg atcaccattg tcggcggtct ggtgatgagt cagctcctga
    23701 cgctgtatac cacgccggtg gtatatctct ttttcgaccg tctgcggctg cgtttttcgc
    23761 gtaaatctaa acaaacggta accgagtaaa tgacagatct tcccgacagc acccgctggc
    23821 aattgtggat tgtggctttc ggcttcttta tgcagtcgct ggacaccacc atcgtaaaca
    23881 ccgcccttcc ctcaatggcg caaagcctcg gggaaagtcc gttgcatatg cacatggtca
    23941 ttgtctctta tgtgctgacc gtggcggtga tgctgcccgc cagcggctgg ctggcggaca
    24001 aagtcggcgt gcgcaatatt ttctttaccg ctatcgtgct gtttactctc ggttcactgt
    24061 tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc acgcgcgtta cagggcgttg
    24121 gcggtgcgat gatggtgccg gtcggcaggt tgacggtgat gaaaatcgta ccgcgcgagc
    24181 aatatatggc ggcgatgacc tttgtcacgt tacccggtca ggtcggtccg ctgctcggtc
    24241 cggcgctcgg cggtctgctg gtggagtacg cgtcgtggca ctggatcttt ttgatcaaca
    24301 ttccggtggg gattatcggt gcgatcgcca cattgatgtt aatgccgaac tacaccatgc
    24361 agacgcggcg ctttgatctt tccggctttt tattgctggc ggttggcatg gcggtattaa
    24421 ccctggcgct ggacggcagt aaaggtacag gtttatcgcc gctggcgatt actggcctgg
    24481 tcgcagttgg tgtggtggca ctggtgcttt atctgctgca cgccagaaat aacaaccgcg
    24541 ctcttttcag tctgaaactg ttccgcactc gtaccttttc gctgggcctg gcggggagct
    24601 ttgccggacg tatcggtagc ggcatgttgc cctttatgac gccggttttc ctgcaaattg
    24661 gccttggctt ctcaccgttt catgccggac tgatgatgat cccgatggtg cttggcagca
    24721 tggggatgaa gcggattgtt gtacaggtgg tgaatcgctt tggttatcgt cgggtactgg
    24781 tagcgaccac gctgggtctg tcgctggtca ccctgttgtt tatgactacc gctctgttgg
    24841 gctggtacta cgttttgccg ttcgtcctgt ttttacaagg gatggtcaac tcgacccgtt
    24901 tctcctccat gaacaccctg acgctgaaag atctcccgga caatctggcg agcagcggca
    24961 acagtctgct gtcgatgatt atgcaattgt cgatgagtat cggcgtcacc atcgccgggc
    25021 tgttgctggg actttttggt tcgcagcatg tcagcgtcga cagcagcacc acacaaaccg
    25081 tctttatgta cacctggctt agcatggcgt ttatcatcgc ccttccggcg ttcatctttg
    25141 ccagagtgcc gaacgatacg catcaaaatg tagctatttc gcggcgaaaa aggagcgcgc
    25201 aatgaagttc tggcgacctg gtattaccgg caaactgttt ctggcgattt tcgccacctg
    25261 cattgtcttg ctgatcagta tgcactgggc ggtgcgtatc agttttgagc gtggctttat
    25321 tgattacatc aagcatggta atgaacagcg attacaactg ttaagtgatg cgcttggcga
    25381 gcagtatgcg cagcatggca actggcgctt cctgcgcaac aatgatcgct ttgtctttca
    25441 gatcctgcgt tcatttgaac acgataattc ggaagataaa cccggcccgg gtatgccacc
    25501 acacggctgg cgtactcagt tctgggtggt tgatcaaaac aacaaggtgc tggttggtcc
    25561 gcgagcgccg attccacctg acggtacacg gcgacccatt ctggtcaacg gtgcggaagt
    25621 gggcgcggtg attgcctccc ctgttgagcg gctgacccgc aatactgata tcaatttcga
    25681 taaacaacag cggcaaacca gctggctgat tgtcgccctg gcaacgttac tcgcggcact
    25741 cgctactttt ctgctggcgc gcggtttgct ggcaccggta aaacgacttg tcgatggcac
    25801 gcacaaactg gcggcgggcg atttcactac ccgcgtaacg cccaccagtg aagatgaact
    25861 gggcaaactg gcgcaagact tcaaccagct cgccagcacg ctggagaaaa accagcaaat
    25921 gcggcgcgat tttatggccg atatttctca cgaactgcgt acgccattag cggtgctgcg
    25981 cggtgaactg gaagccattc aggatggcgt gcgcaaattc acgccggaga cggtggcgtc
    26041 tttacaggcg gaggtcggta cactgaccaa actggttgac gatcttcatc agttgtcgat
    26101 gtctgatgaa ggcgctctcg cctatcaaaa agcaccggta gatttgatcc cactgctgga
    26161 agtggcaggc ggcgcatttc gcgaacgatt tgccagccgt ggcctgaaac tgcaattttc
    26221 cctgccagac agtatcaccg tatttggcga tcgcgaccgt ttaatgcagt tattcaataa
    26281 cttactggaa aacagcctgc gctacactga cagcggcggc agcctgaaaa tctctgccga
    26341 gcagcacgac aaaacggtgc gcctgacctt tgccgacagt gcgccaggtg tcagtgacga
    26401 tcagctacaa aaattgtttg aacgttttta tcgcaccgaa ggttcccgca accgcgccag
    26461 cggcggttcc gggctggggc tggcgatttg cctgaacatt gttgaagcac ataatggccg
    26521 cattattgcc gcccattcgc cttttggcgg ggtaagcatt acagtagagt taccgctgga
    26581 acgggattta cagagagaag tatgaccgag ttaccaatcg acgaaaacac accgcgtatt
    26641 ttgatcgtgg aagatgaacc gaagctggga cagttgctca ttgattatct gcgtgctgcg
    26701 agctatgcgc cgacgcttat cagccacggc gatcaggtac tgtcgtacgt gcgccagacg
    26761 ccgccggatc tgatcctgtt ggatctgatg ctccctggca ccgatggcct gacgctgtgc
    26821 cgggaaattc gtcgtttttc tgacattcct atcgtgatgg tgacagcaaa aatcgaagag
    26881 atcgaccgcc tgctagggct ggagattggc gcagatgact acatttgcaa gccgtacagc
    26941 ccacgggaag tggtagcgcg tgtcaaaacc attttgcgcc gttgcaaacc gcagcgtgag
    27001 ttgcagcaac aggatgctga aagcccgttg attatcgacg aaggtcgttt tcaggcttca
    27061 tggcgcggta aaatgcttga cctgacgcct gcggaatttc gtctgctgaa aacgctctct
    27121 catgaaccag gaaaagtgtt ctcccgcgag caattgctca atcatcttta tgacgactac
    27181 cgcgtagtaa ccgaccgtac catcgacagc cacattaaaa acctgcgccg caagctggaa
    27241 tctctcgacg ccgaacagtc atttatccgc gccgtttacg gcgtcggtta ccgctgggaa
    27301 gccgacgcct gccgcatcgt ttagttttag cgacattact ttgttagccg gagatggcat
    27361 tcagaccaga tccggcaaca ttatcccacg catggtcagc aaactgacat tatctctccg
    27421 ccctggcagg catcagaaaa tcgctcatac tttaatcggt aaacagcacc tttagatgct
    27481 gttttcgata cgcaatttca atcaaggagt cattatggct ggttggtttg aactcagtaa
    27541 gagcagtgat agtcagttcc ggtttgtgct aaaagcgggc aatggtgaga cgatcctcac
    27601 cagcgagctg tatacctcaa aagcctctgc ggaaaagggc atcgcgtcgg tgcgtagcaa
    27661 cagcccgcaa gaagaacgct atgagaaaaa aacggcaagc aacggcaaat tctatttcaa
    27721 tctgaaagcc gccaatcatc aaattatcgg ctccagccag atgtatgcca ccgcgcaatc
    27781 tcgtgaaacc ggaattgcct ccgttaaagc caatggcaca agccagacgg tgaaagacaa
    27841 tacgtaatta cgatgccggg cgtgggttgt tggcgtccgg cgctttacca gatcccccct
    27901 acagcgctac aatgcccgcc cttaaagtgg gggcactccc ctaaccgctt catcaggtga
    27961 agcggatctg acctgtcatc agaacgagag aattatgttt aaaccggaac tcctttcccc
    28021 ggcgggaacg ctgaaaaata tgcgttacgc tttcgcttat ggcgcagatg ctgtttatgc
    28081 gggccagccg cgttactccc tgcgtgtgcg caacaacgaa ttcaaccacg aaaaccttca
    28141 gctcggcatc aatgaagccc acgcgctggg gaaaaagttt tatgtcgtgg tcaacattgc
    28201 accgcacaac gccaagctga aaacctttat ccgtgacctg aaaccggtgg tggaaatggg
    28261 gccggatgcg ctgattatgt ccgatccagg gctgattatg ctggtgcgtg agcacttccc
    28321 tgaaatgccg atccatctct cggtacaggc taacgccgtg aactgggcga cggtgaaatt
    28381 ctggcagcaa atgggactga cccgcgtgat cctctctcgc gagctgtcgc tggaagagat
    28441 tgaagagatc cgcaatcagg tgccggatat ggagatcgag atcttcgttc acggcgcact
    28501 gtgcatggcc tactccggtc gctgcctgct ctctggctat atcaacaagc gcgatccgaa
    28561 ccagggtacc tgcaccaacg cctgccgctg ggagtacaac gtccaggaag ggaaagaaga
    28621 tgacgttggc aacatcgtac acaagtacga gccgattccg gtgcaaaatg ttgagccgac
    28681 gctgggtatc ggcgcgccaa ccgacaaagt gtttatgatc gaagaagccc agcgtccggg
    28741 cgagtatatg accgcgtttg aagatgagca cggcacttac atcatgaact cgaaagatct
    28801 gcgcgccatc gcccatgtag aacgcctgac caaaatgggc gtgcattcgc tgaaaatcga
    28861 aggccgtact aaatctttct actattgcgc acgcaccgca caggtttatc gtaaagctat
    28921 cgatgatgcc gctgcgggca aaccgttcga taccagcctg ctggaaactc tggaaggtct
    28981 ggcgcatcgt ggctataccg aaggtttcct gcgtcgtcat actcacgacg attatcagaa
    29041 ctacgaatac ggttattcag tttctgaccg ccagcagttt gttggtgagt ttaccggtga
    29101 gcgcaagggg gacctcgcgg cggtagcggt gaaaaataaa ttctccgttg gcgacagcct
    29161 tgagctgatg acgccgcaag gcaacattaa ctttaccctt gagcacatgg aaaacgccaa
    29221 aggcgaagct atgccggtag caccaggcga tggttatact gtgtggctcc cggtgccgca
    29281 ggatcttgag ctaaattacg cgctgctgat gcgtaatttc tccggggaaa ctacgcgtaa
    29341 cccccacggt aagtgattaa tttcgattat ttttcccgga tggaaaattc ttagaaaccg
    29401 atcacataca gctgcattta ttaaggttat catccgtttc gctgaaaaac ataacccata
    29461 aaatgctagc tgtaccagga accacctcct tagcctgtgt aatctccctt acacgggctt
    29521 attttttatg cataagccct atccctgata accgtcttcc attgaccaca tcgatagaat
    29581 ctcccttcat agcacgatgc ctttcacgta acggcatcgt gctcgcacag gttccggcta
    29641 agcacaacca gaacgcgcat gtttgacgct taccaaaaaa tattctcact ctccacattt
    29701 gaatgtcaga cgagcgacgc catgtaatcc tgcaccttct gtcttcaggt caactatctg
    29761 catttttttg ccctgagtaa cacagaaatg ggctgcatca ttttttacta tattttctgc
    29821 accagatatt ctgcccctgg ctaaagaagc ttcggcttcg gtgtagtatt ggttatcgag
    29881 tttacgctga atattacttt tatatgcaag accaaattta ccgatacttg tctcatcatt
    29941 atgcacagca caaccagaca taagaaaaac actaattaat gatatagcag ctatcttttt
    30001 catctcacct tcccccatta aataccaacg acactctcta gtgtttaa
//
LOCUS       DAZKIF010000052        30008 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZKIF010000052 DAZKIF010000000
VERSION     DAZKIF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.052"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(376..2205)
                     /gene="glmS"
                     /locus_tag="ACHOTW_002271"
     CDS             complement(376..2205)
                     /gene="glmS"
                     /locus_tag="ACHOTW_002271"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9844378.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2367..3737)
                     /gene="glmU"
                     /locus_tag="ACHOTW_002272"
     CDS             complement(2367..3737)
                     /gene="glmU"
                     /locus_tag="ACHOTW_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9844379.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(4089..4508)
                     /gene="atpC"
                     /locus_tag="ACHOTW_002273"
     CDS             complement(4089..4508)
                     /gene="atpC"
                     /locus_tag="ACHOTW_002273"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9844380.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQLGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4529..5911)
                     /gene="atpD"
                     /locus_tag="ACHOTW_002274"
     CDS             complement(4529..5911)
                     /gene="atpD"
                     /locus_tag="ACHOTW_002274"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9844381.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5938..6801)
                     /gene="atpG"
                     /locus_tag="ACHOTW_002275"
     CDS             complement(5938..6801)
                     /gene="atpG"
                     /locus_tag="ACHOTW_002275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9844382.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6852..8393)
                     /gene="atpA"
                     /locus_tag="ACHOTW_002276"
     CDS             complement(6852..8393)
                     /gene="atpA"
                     /locus_tag="ACHOTW_002276"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9844383.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8406..8939)
                     /gene="atpH"
                     /locus_tag="ACHOTW_002277"
     CDS             complement(8406..8939)
                     /gene="atpH"
                     /locus_tag="ACHOTW_002277"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9844384.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8954..9424)
                     /gene="atpF"
                     /locus_tag="ACHOTW_002278"
     CDS             complement(8954..9424)
                     /gene="atpF"
                     /locus_tag="ACHOTW_002278"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9844385.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9486..9725)
                     /gene="atpE"
                     /locus_tag="ACHOTW_002279"
     CDS             complement(9486..9725)
                     /gene="atpE"
                     /locus_tag="ACHOTW_002279"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP9844386.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9772..10587)
                     /gene="atpB"
                     /locus_tag="ACHOTW_002280"
     CDS             complement(9772..10587)
                     /gene="atpB"
                     /locus_tag="ACHOTW_002280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9844387.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10596..10976)
                     /gene="atpI"
                     /locus_tag="ACHOTW_002281"
     CDS             complement(10596..10976)
                     /gene="atpI"
                     /locus_tag="ACHOTW_002281"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP9844388.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11593..12216)
                     /gene="rsmG"
                     /locus_tag="ACHOTW_002282"
     CDS             complement(11593..12216)
                     /gene="rsmG"
                     /locus_tag="ACHOTW_002282"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9844389.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12280..14169)
                     /gene="mnmG"
                     /locus_tag="ACHOTW_002283"
     CDS             complement(12280..14169)
                     /gene="mnmG"
                     /locus_tag="ACHOTW_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9844390.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14548..14991)
                     /gene="mioC"
                     /locus_tag="ACHOTW_002284"
     CDS             complement(14548..14991)
                     /gene="mioC"
                     /locus_tag="ACHOTW_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP9844391.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(15081..15539)
                     /gene="asnC"
                     /locus_tag="ACHOTW_002285"
     CDS             complement(15081..15539)
                     /gene="asnC"
                     /locus_tag="ACHOTW_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP9844392.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15691..16683
                     /gene="asnA"
                     /locus_tag="ACHOTW_002286"
     CDS             15691..16683
                     /gene="asnA"
                     /locus_tag="ACHOTW_002286"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP9844393.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16688..18139)
                     /gene="viaA"
                     /locus_tag="ACHOTW_002287"
     CDS             complement(16688..18139)
                     /gene="viaA"
                     /locus_tag="ACHOTW_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP9844394.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(18133..19629)
                     /gene="ravA"
                     /locus_tag="ACHOTW_002288"
     CDS             complement(18133..19629)
                     /gene="ravA"
                     /locus_tag="ACHOTW_002288"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP9844395.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19852..21720
                     /gene="kup"
                     /locus_tag="ACHOTW_002289"
     CDS             19852..21720
                     /gene="kup"
                     /locus_tag="ACHOTW_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP9844396.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21887..22306
                     /gene="rbsD"
                     /locus_tag="ACHOTW_002290"
     CDS             21887..22306
                     /gene="rbsD"
                     /locus_tag="ACHOTW_002290"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9844397.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22314..23819
                     /gene="rbsA"
                     /locus_tag="ACHOTW_002291"
     CDS             22314..23819
                     /gene="rbsA"
                     /locus_tag="ACHOTW_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP9844398.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23824..24789
                     /gene="rbsC"
                     /locus_tag="ACHOTW_002292"
     CDS             23824..24789
                     /gene="rbsC"
                     /locus_tag="ACHOTW_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP9844399.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24814..25704
                     /gene="rbsB"
                     /locus_tag="ACHOTW_002293"
     CDS             24814..25704
                     /gene="rbsB"
                     /locus_tag="ACHOTW_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP9844400.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25830..26759
                     /gene="rbsK"
                     /locus_tag="ACHOTW_002294"
     CDS             25830..26759
                     /gene="rbsK"
                     /locus_tag="ACHOTW_002294"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9844401.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26763..27755
                     /gene="rbsR"
                     /locus_tag="ACHOTW_002295"
     CDS             26763..27755
                     /gene="rbsR"
                     /locus_tag="ACHOTW_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP9844402.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27721..29148)
                     /gene="mdtD"
                     /locus_tag="ACHOTW_002296"
     CDS             complement(27721..29148)
                     /gene="mdtD"
                     /locus_tag="ACHOTW_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP9844403.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(29171..29863)
                     /gene="yieP"
                     /locus_tag="ACHOTW_002297"
     CDS             complement(29171..29863)
                     /gene="yieP"
                     /locus_tag="ACHOTW_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9844404.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7258 a         7656 c         7636 g         7458 t
ORIGIN      
        1 tagaaaacag tcttattttt agtaaaccac actttcaaat tatatttttt gcctatattt
       61 gtggaaagac tatacattta cagtgataat ttattatcac acattgttac atagttaaat
      121 aaaattacat attgatgatt gttttacaag cgattcttga atgaacaaaa cgtgctattc
      181 attacaaagt acatcaatta tggcaattgt ttgtcaatga ttcgaggtat ttaattacta
      241 ctacggttaa aacaagtgtt caacggtgcc ccaaataata gctgaagtta attatatgga
      301 atgaggttag attattaatg ctggtaagta aaagggccgc caaaaggcag cccttcgttc
      361 agcaaatgaa taatattact caaccgttac cgattttgcc aggttacgcg gctggtcaac
      421 gtcggtgcct ttgatcagcg cgacgtgata agccagtaac tgcagcggaa cggtgtagaa
      481 gatcggtgca atcacctctt ccacatgcgg catctcgatg atgtgcatgt tatcgctact
      541 tacaaaaccc gcatcctgct cggcgaagac atacaattgg ccgccacgcg cgcgaacttc
      601 ttcaatgttg gatttcagtt tttccagcaa ttcgttgttc ggcgcaacga cgataaccgg
      661 catatcggcg tcaatcagcg ccagcggacc gtgtttcagt tcgcctgcag cgtaggcttc
      721 agcgtgaata taagagatct ctttcagctt caatgcgcct tccagcgcga ttgggtactg
      781 atcgccacgg cccaggaaca gcgcgtgatg tttgtcagag aaatcttctg ccagagcttc
      841 aatgcgtttg tcctgagaca gcatctgctc aatacggctc ggcagcgcct gcagaccatg
      901 cacaatgtca tgttcaatgg aggcatccag acctttcagg cgagacagct tcgccaccag
      961 catcaacagc acagttaact gagtggtgaa tgctttagtg gatgccacgc cgatttccgt
     1021 acccgcgttg gtcatcagcg ccagatcgga ttcgcgcacc agagaagaac ccggaacgtt
     1081 acagattgcc agcgaaccaa ggtaacccag ctctttcgac agacgcaggc ccgccagggt
     1141 atccgcggtt tcgccagact gtgacaaggt gatcatcagg ctgttacgac gcacggcaga
     1201 tttacgatag cggaattcag aggcgatttc gacgtcgcac ggaatacctg ccagcgattc
     1261 aaaccagtaa cgggaaacca taccggagtt ataagaagta ccacaggcga ggatctgaat
     1321 atgctcaacc ttcgacagca gttcgtcggc gttcggtccc agctcgctta aatcaacctg
     1381 accgtggctg atgcgcccgg taagggtgtt tttgatcgcg ttcggctgtt cgtagatctc
     1441 tttctgcatg tagtgacggt aaataccttt atcgcccgcg tcatattgca gattggattc
     1501 gatatcctga cgttttactt ccgcgccagt tttatcgaag atgtttaccg aacggcgagt
     1561 gatttccgca atatcgccct cttcaaggaa gataaagcga cgggtcaccg gcagcagcgc
     1621 cagctggtca gaagcgataa agttttcacc catacccaga ccaatcacca gcggactacc
     1681 agaacgtgct gccagcaggg tatccgggtg acgggagtcc atgatcactg taccgtacgc
     1741 accacgcagt tgcgggatag cacgcagaac agcctcacgc agagtaccgc cctgtttcag
     1801 ctcccagttc accagatggg caatcacttc ggtgtcggtt tcagaaacga aggtatagcc
     1861 acgcgctttt aactcttcgc gcagcggttc atggttttcg atgatgccgt tatgcaccac
     1921 cacaatgtgt tcagaaacat gcggatgcgc attcacttct gaaggttcac cgtgggtcgc
     1981 ccagcgagtg tgagcaatac cggtgccgcc atgcagagga tgttcttccg ctgcctgagc
     2041 cagcatctgg actttaccga ggcgacgcag gcgggtcata tgaccttccg catcaacaac
     2101 ggccagaccg gcagagtcat atccgcggta ttccagacga cgtaaacctt caagaaggat
     2161 ttctgctaca tcacgttgcg cgatcgcgcc aacaattcca cacatagttt ttgattccga
     2221 tttatatcgt tgtcggtcaa cctgtatgcc cgttttttcg ggcgccccga gccttgtaga
     2281 gagtggggtt atttttatag ttactgcttg tgggagggag attatgttat ctcctcatcc
     2341 catgtgaccg gattacccgg ccagaatcac tttttcttta ccggacgacg ccagccttct
     2401 ttctgagtct gcggcacacg gctgatagct aatgcatttt cgccgacatt acgcgtcaca
     2461 gttgtacccg cagcaatggt cgcgcctttg cctactgtta ccggggccac cagctgagtg
     2521 tcggaaccga caaacacatc gtcgccgata atggtcttaa atttattcgc accatcgtag
     2581 ttgcaggtaa tggttcccgc gccgatgtta acgttatcgc caatttccgc atcgccaagg
     2641 taagtcagat gaccagcttt cgagccttta cccagacgtg cttttttcat ctcaacaaag
     2701 ttaccgacgt gtgcaccttc caacaactca gcaccaggac gcaaacgggc aaacgggcca
     2761 atggtacagg ccgccgccag attcgcgtct tccacgacgg tatatgggct aatttcgcaa
     2821 tcatcgccaa tcacgctgtt tttaatcacg caaccggtgc cgattttcac gcgatggccg
     2881 agagtcacgt tgccctcgat gataacgtta gtatcaattt caacatcgcg cccgtgagta
     2941 agcgtaccgc gcagatcaaa acgcgccgga tcgcgcagca taacgcctgc taacagcagt
     3001 ttttcagcct gttcggactg gtaaacgcgc tccagacggg agagttgcag gcggttattc
     3061 acgccttcta cttcgcttaa acgttgcgga tgaacggcga cgatttcacg cccttcctga
     3121 tacgccagcg caataatgtc ggtgatgtag tattcgccct gcgcattatt gttggtcagc
     3181 ttcgccagcc agcgtttcat atctgcgccg ttagcgatca gaatgccggt gttgatctcc
     3241 tgaatctgac gctgctcgtc ggtggcatct ttgtgctcaa caatgccggt aactttgccg
     3301 ttttcacggg tgatacgtcc ataaccggtc ggatcatcca gtttcaccgt cagcagacca
     3361 atgccaccct gcggtttagc atcacgcaga cgctggagtg tttcgacaga gatcagcggc
     3421 acgtcgccgt agagcattaa aatgtcttca tcatcggcaa agaaaggtgc ggcctgctgc
     3481 atggcatgac ccgtacccag ctgctctgcc tgaagcaccc agttcaggtt gtcgtctttc
     3541 agcgcctgtt ttagcagatc gccaccgtga ccgtacacca ggtgaacgtg cgctgcgcct
     3601 aattcattcg cagcatcaat gacatgctga accatcgctt tcccggcaag ggtatgcagc
     3661 actttcggaa gatcggaata catgcgcgtg cctttacctg cggcaaggat cactacgctc
     3721 atagcattat tcaacatacg cgtcctgact gtaatttgag aacgaattta aaccgcttca
     3781 ccttgaaaaa actacatttt tttcatcgtg aaatggacag aggataaatt gttcaataac
     3841 ggattatccc cgctgacgaa agcgccattt tcgaccatcg tcacgccttt tgtctgcaga
     3901 aaattaaggc agtaccttca tttaagcgtc aggtggatgt ttttgctctt atttcgatca
     3961 atgaataaac gggaaaacag ggggttttat atcagcagga tctatgtgaa cgctattcag
     4021 gacgggtcac acgcgcaaaa aaaagccagc ctgtttccag actggctttt gtgcttttca
     4081 agccggtgtt acatcgcttt tttggtcaac tcgataacgc gcagctgcgc gatcgctttg
     4141 gccagttccg cagacgcctg agcgtaatct acgtcgccgt gagagctgct aatgtgctct
     4201 tcagccttac gtttcgcttc catggctcgc gcttcgtcga gatcctggcc gcgaattgcg
     4261 gtgtcggcca gaacggtcac gttgccaagc tgcacttcaa gaatgccgcc agacagatag
     4321 ataaactctt cgtgaccgtg ctgtttcacg atgcgaatca taccaggctt aatggcggtg
     4381 agcagcggtg cgtggccagg gtagatcccc agttcacctt cgctacccgt tacctggatt
     4441 ttctcgacca gaccagagaa catttgttgc tctgcgctga cgacgtccag gtggtaagtc
     4501 attgccatat caccctccga ttaaggcgtt aaagtttttt ggctttttcc acagcttctt
     4561 cgatggaacc gaccatgtag aacgcctgct ccggcaggtg atcgtattcg ccttccatga
     4621 tgcctttaaa gccacggatg gtgtctttca gggagacgta tttacccgga gaaccggtga
     4681 atacttctgc cacgaagaac ggctgggaca ggaagcgctg gatcttacga gcacgcgcta
     4741 ccaccagttt gtcttcttca gacagttcat ccatacccag gatggcgatg atgtctttca
     4801 gttcctgata acgttgcagg atggactgaa cgccacgcgc ggtgtcgtag tgttcctgac
     4861 caaccaccag cgggtccagc tgacggctgg tggagtccag cgggtcaacg gccgggtaga
     4921 tacccagaga cgcgatctga cggctcagta ccacggttgc gtcaaggtgc gcaaaggtgg
     4981 ttgccggaga cgggtcagtc aagtcatccg caggtacgta tactgcctgt acggaggtga
     5041 tagaaccggt tttggtggag gtgatacgtt cctgcagaac gcccatctct tccgccaggg
     5101 tcggctgata acctaccgct gaaggcatac ggcccagcag tgcggatact tccgtaccgg
     5161 ccagggtgta acgatagatg ttgtcaacga acagcagaac gtcacgacct tcgtcacgga
     5221 atttctcagc catggtcaga ccggtcagag ctacgcgcag acggtttccc ggcggctcgt
     5281 tcatctggcc atacaccagg gatactttat cgataacgtt ggagtcggtc atttcgtggt
     5341 agaagtcgtt cccctcacga gtacgttcac ctacgcccgc aaacacagag taaccggagt
     5401 gctcgatcgc gatgttacga atgagctcca tcatgtttac ggttttacct acacccgcac
     5461 caccgaacag accaacttta ccgcccttag cgaacggaca catcaggtcg ataactttga
     5521 taccggtttc cagcagttcc tgagagtttg acagctcttc gtaggaaggt gctgcgcggt
     5581 gaatcgccca acgctcttct tcaccgatct cgcctttcat gtcgaccggt tcacccagta
     5641 cgttcatgat acggcccaga gtcgctttac ctaccgggac ttcgatcggg tgttcgaggt
     5701 cttttacatc cagaccgcga cgcagaccgt cggaggaacc cattgcgatg gtacgcacga
     5761 taccgccgcc gagctgctgc tgaacttcca gcaccagacg ctcattacca ttttgcacct
     5821 caagagcatc gtacacgcgc ggtacggcat cctgagggaa ttcgacgtca actacggcgc
     5881 cgattacctg gacaatcttt ccagtagcca tcttaaatcc tctacgaaat aacctgttta
     5941 aaccgcggcg gcccccgaga cgatctcggt gagttcctga gtaatgctgg cctgacgagc
     6001 tttgttgtat accaactgca gctctttaat caggctgccg ccattgtcgg tcgcggcttt
     6061 catcgccacc atacgggcgg cctgctcgct ggccaggttt tcaaccacgc cctgataaac
     6121 ctgagattcg acataacgac gcagcagggt atccagcaac gccttcggat cgggttcgta
     6181 caggtaatcc caggatttat gtttcagatc atcatcatct gatgccggta acggcagcag
     6241 ctggctgatg gtcggaacct gagacatggt gttaataaat ttgttgctga caatgtaaag
     6301 cttgtccaga cggccttcgt cgtaggcctg caacatcact tttaccggac cgatcagttc
     6361 ggacagggaa gggttatccc ccatgccggt gacctgggca acaacattgc cgcccacgga
     6421 gttgaagaac gacacgcctt tcgagccgat cattgcgagg tcgcattgaa cgcctttgtc
     6481 ggtccaggtc ttcatttccg ccagcagttt tttgaacagg ttaatgttca aaccaccgca
     6541 caaaccacgg tcggtcgaca ccaccaggta gcccacgcgt ttaacgtcgc ggtcttccag
     6601 gtaagggtgc ttatattcca gattaccgtg tgcaaggtga ccaatcactt tgcgcatggt
     6661 ttctgcataa ggacggctgg ccgccatgcg atcctgcgat ttacgcattt tggaagcggc
     6721 gaccatctcc atcgctttag tgatcttttg cgtgttctgg acgcttgcga tcttactacg
     6781 tatctctttt gcgccggcca tgagcttctc ctcaatgcct tgcggcctgc cctaaggcaa
     6841 gccgccagac gttaccagga ttgggttgct ttgaaggaat cgaggatgcc tttcagcttg
     6901 ccttcgattt cgtcgttgta gccaccggtc tggttgatct cttgcatcaa cggagcgtga
     6961 tcacggtcga cgtaagccag cagagcggct tcgaagctgc cgattttcga cagttcaaca
     7021 tccgccaggt aaccacgttc tgctgcgaac agaaccagag actgctgcgc aacggacatc
     7081 ggcgcatact gtttctgttt cagcagttcg gtcacttttt gaccgtggtc aagctgctta
     7141 cgtgttgcat cgtcaaggtc ggatgcaaac tgagagaacg ctgccagttc acgatactgt
     7201 gccagagcgg tacggatacc accggacagt tttttcatga tcttggtctg tgctgcacca
     7261 ccaacacggg atacggaaat acccgggtta accgcaggac gaataccggc gttgaacagg
     7321 ttggtttcca ggaagatctg accatcggta atggagatta cgttggtcgg aacgaacgca
     7381 gaaacgtcac ccgcttgagt ttcgataatc ggcagcgcgg tcagtgaacc ggttttccct
     7441 ttcacttcac ctttggtgaa agcttcaacg tattcggcgt taacacgcgc agcacgctcc
     7501 agcagacgag agtggaggta gaatacgtcg cccgggaatg cttcacgtcc tggcggacga
     7561 cggagcagca gcgagatctg acggtaagca acagcctgtt tagacagatc atcgtaaatg
     7621 atcagcgcat cttcaccgcg gtcacggaag tattcgccca ttgcgcaacc ggcatacggt
     7681 gccaggtatt gcagtgcagc ggattcagac gcggttgcta ccacaacgat ggtgttagcc
     7741 agtgcgccgt gctcttccag tttacgtacc acgttagaaa tggtggacgc tttctggccg
     7801 atagcgacat agatacattt gataccggaa tcgcgctggt tgatgatggc atcgatagcc
     7861 agtgcggttt taccggtctg acggtcaccg atgatcaatt cacgctgacc acgaccgatt
     7921 gggatcatgg agtcaacggc tttataaccg gtctgtaccg gctgatctac ggactgacgt
     7981 tcgataacgc ccggagcgat tgcttctaca gcagagaagc cgtcgtgatc cagcggacct
     8041 ttaccgtcga ttggtgcacc cagagtgtta accacacggc ccagcaggcc acggccaacc
     8101 ggaacttcca ggatacggcc agtacactta actttcatgc cttcggcaag gtcagcatac
     8161 ggacccataa caaccgcacc aacagagtcg cgctcgaggt tcagtgcgat agcgtaacgg
     8221 ttacccggca gggagatcat ttcaccctgc atacaatcgg ccaggccgtg aatgcggata
     8281 acaccgtcac ttacagaaac aatagtacct tcgttgtgag cttcactcac aacattgaac
     8341 tgagcaatgc gctgcttgat cagttcgctg atttcggtgg aattcagttg catgctccag
     8401 tccccttaag actgcaagac gtctgcaagg cgctcaagac gaccgcgtac gctgccatca
     8461 atgaccatat cacccgctcg gatgataacg cctgccatta cagacttatc gattttgcaa
     8521 ttcagcttaa ctttgcgtga cagacgtttt tccatcgcag cagaaatttt cgcgagctgt
     8581 tgttcactca gtgcggcagc ggaaatgacg tctacctcag cggtagcctc actcacggca
     8641 cgaaggtgaa taaactgctc caaaacatcc gggagcgcgt taagacgacc attttcagcc
     8701 attacccgaa taaggttctg accgttttcg tccagttgct caccacaaac tgcgataaac
     8761 gactcggcaa gcgtttctgg cgcaagcgcg ccagagagaa gctctgccat ttgttcgttt
     8821 ttggttacct cggcggcaaa cgccagcatg tcctgccagc gttctacact ttggtgttcg
     8881 acggcaaagt caaaagctgc tttggcgtag gggcgagcta ccgtaataaa ttcagacatc
     8941 agcccctccc tccttacagt tcagcgacaa gtttatccac gatgtcgctg ttagcagctt
     9001 catccacgga acgttcgatg atcttctcgg cgccagcaac agccaggata gcaacttgct
     9061 tacgcagctc ttcacgggca cgtttacgct cggcttcaat ttccgcctgc gcctgggcca
     9121 cgattttagt acgttcctgt tctgcctcag ctttcgcttc gtccagaatc tgcgagcggc
     9181 gtttgttcgc ctgctcgatg attacctggg cttccgcttt cgcttttttc agctggtcgg
     9241 tcgcgctggc ctttgcaagg tcaaggtcct tatgtgctcg ttctgcggaa gcaaggccgt
     9301 cagcaatttc tttttgacgt ttttcgatgg ctgccattaa tggcggccat acgtacttca
     9361 tgcagaacag aacgaacagg acaaacgcga tggcctggcc gaggattgtt gcgttaagat
     9421 tcacagcaca atgcctctat ttagttaacg ttctgatatt gctctttaaa taaaagcaac
     9481 gcttactacg cgacagcgaa catcacgtac agacccagac ctacagcgat catcgggata
     9541 gcatccacca gacccataac gataaagaac tgagtacgca gcagaggaat cagatcaggt
     9601 tgacgcgctg cgccttccag gaatttaccc ccgaggatgc cgataccgat cgcagcaccg
     9661 attgccgcca gacccatcat cacagcggca gccatgtaca gcagatccat attcaggttt
     9721 tccatgacag tctccagttt gtttcagtta aaacgtagta gtgttggtaa attaatgttc
     9781 ttcagacgcc atcgacagat agacgatcgt cagaaccatg aagatgaagg cttgcagcgt
     9841 aatgatcagg atgtggaaaa tggcccacgg cacattcagg atccactgtg accaccacgg
     9901 caacagacca gcaatcagaa tgaaaatcag ctcaccggca tacatgttac cgaacagtcg
     9961 caaaccgagt gaaactggtt tggacagcag gcttacccct tcaaggatta agttgacagg
    10021 aatgaacgcc cagtgattga acggctgcag cgtcaactct ttcgtgaagc cgccgatgcc
    10081 tttcattttg atgctgtaga acagaatcag gataaatacg cccagtgcca tagacagcgt
    10141 tacgttcacg tccgcagacg gaaccacacg cagtgcaggc agacccagta catgttcagc
    10201 aatgtacggc agcaggtcga taggcagtaa atccatcagg ttcatcagga atacccagac
    10261 gaagatcgtc agggccagcg gagcaatcag cttgcttttg ccatggtaca tgtctttcac
    10321 gctaccatta acaaagccga tcaccagctc aatcgcggtc tgaaacttac ctggcacacc
    10381 gctggtcgcc tttttggcta cgctacggaa taaaaccagg aacaacagac ccagcaccac
    10441 cgagaagaac atggagtcaa tattgattgt ccagaaggtg gctggggggt tttgtggatc
    10501 caccagcgag aatgtacgca ggtccagctg aaggttattc aggtggtgtc ctatgtaatc
    10561 ctgcggcgtc atattttctg aagccatgat gccttttacc ctttgttgtt aattacagcc
    10621 ggtgccagta tctgaaccac cagcaccaaa acccacgtaa cgatcagcgg caagaatacc
    10681 gcctttaaaa ccgccaacgc caccaccagt aacaccaaca tcgccagaac tttgaaagct
    10741 tcaccaaatg cgaatgtcca ggccacccgg cctttcgctg gtgtatgcgc ctggtgacgc
    10801 caggcaaata tcataaacaa aacgttaggc agaaagactg ccaggccccc gcttattgca
    10861 gagacgcccc agaaggggtc tttgaggctg aacagcaatc cacttgctat caccaccagt
    10921 aactgaacga gcagaagctt ccgagcaacg tttcgactca cgagcgacac agacatcacg
    10981 tttttcactc ctgctccctt cgaggtatgc cgcgtgtcgt ataaaacttt ctttaaggct
    11041 tagagtcaag catcaaaaag cggtcaaatt atacggtgcg cccccgtgat ttcaaacaat
    11101 aagtagccaa aaggtgaata aatgtttaaa tatttttcca gtgcatacaa ttgcgacttt
    11161 tctactaacc ctgttcgatc atgaaaaact gtaaataacg cgtaaacact ggtgataaag
    11221 cgtgcttcag atcacatatt gcgcatgttc gcgcacagca tatttattta cttggcaaat
    11281 gatgcctttg caagtttatg atatttcagt ctaaaaacag atactgtttt aataaatgac
    11341 atttacacaa caaaaaccac ccattgacat ttttaataat gttttaacag ccaatgatgg
    11401 ttcttagcgc cgatttttag cagactgata ttttcactaa tgacttattt tctgcttacc
    11461 aaaaaaagcc acgttatctt gttgatgcaa aagagtgaac gtggcgttaa atgtaaccag
    11521 ttatatcagt agaaaacctg gttgttgtta acagtctaac cggtcaattt tttatgattt
    11581 ttttgataaa aattaaattt tatttgcttt aatcaccacc agatgacgtt cgccatccag
    11641 ggctggaacc tgaagtttaa ccactgattc gacctgatat tcttcgggca acaaagcgat
    11701 ttcatcttcc ggcatttgcc ctttcagcgc gtagaaacgg ccttgctcac caggaagatg
    11761 gtggcaccag ctcaccatat cgttcagaga ggcaaaagcg cggctaatta cgccatcaaa
    11821 tggcggctct gaaggaaact cttctaccct gctctgtact ggctcaatat tctccagttt
    11881 aagctcatgt tgcacctgac gaaggaaacg cacgcgttta ccaaggctat ccaacagagt
    11941 gaaatgggct tcaggacgca cgatagagag tggaatgcca ggcagtccag gaccggtgcc
    12001 gacatcgata aaccgttcac cttgcagata cggtgccacc acaatgctat cgagaatatg
    12061 gcgtaccagc atctcattag gatcgcggac cgaagtcagg ttgtacgctt tgttccattt
    12121 atgcagcata ttcacgtagg caataagctg gtttttctgg tgatcggtaa gcgaaatacc
    12181 tgcgtctttc agcagtaagg agagtttgtt gagcacggtg attacctgtt cttgatgcgt
    12241 tgcctggtaa gcgggggctt accaggcatt tttaatgcgt tatgcgctac gacgcagcat
    12301 accctgtttt ttcagccaca ccagcagaat ggagatggcc gcaggcgtga cgccagaaat
    12361 acgtgaagcc tggccgatag aggccggttt gtgatcgtta agtttggcga tcacttcgtt
    12421 agaaagaccg gatacctggc ggtaatccag tgtcgcgggt agcaaggtgt tctcgttacg
    12481 cagctgcttt tcgatctcat cttgctggcg cgcgatataa ccttcgtatt taacctgaat
    12541 ctcaacctgt tccgccgcct gttcgtctgt caacgcaggg gcaaacggcg tcagcgtggt
    12601 taatttttca taagtcattt ccggacgacg cagcagatct tcaccactgg cttcacggga
    12661 aagcggcgca gtcaggtgag cattcacttc ggctgcagct tccgccgacg gggttaccca
    12721 ggtcgatttc agacgctgac gctcacgctc gatattctca agtttctcgt taaagcgcgc
    12781 ccaacgttcg tcatccacca ggcccagttc acgaccgatt tcagtcaaac gcagatccgc
    12841 attatcttcg cgcagcatca gacgatattc tgcgcgcgag gtaaacatac gatacggttc
    12901 tttggttcct aaagtgcaca ggtcatcaac cagtacgccg agataggcct gagaacgcgc
    12961 cggagcccaa ccttctttgt catcagacag acgggcagcg ttaagaccgg ccagcaaacc
    13021 ttgcgcagcg gcttcttcgt aaccggtagt gccgttaatc tgaccagcaa agaacagccc
    13081 ctggataaac ttgctctcca gcgtcggttt caggtcgcga ggatcgaaga agtcatactc
    13141 aatggcataa cccggacgca cgatcttcgc gttttccatc ccctgcatag agcggacgat
    13201 ttgcatctgc acatcgaacg gcaggctggt ggagataccg ttcggataaa tttcgttaga
    13261 agtcagccct tccggttcaa ggaagatctg atgctgattt ctgtcggcga agcgcatgac
    13321 tttgtcttcg atcgacgggc agtagcgtgg gccgacacct tcgatcaccc ctgcgtacat
    13381 tgggctacga tcgaggttac tgcggatcac atcatgggtt ttctcgttgg tatgggtgat
    13441 ataacacggc acctgctgtg gatgctggga cgcattgccc ataaaagaga ataccggcat
    13501 tgggttatcg ccatgctgtt gcgcaagcac gctaaagtcg atggttcgag catcaatacg
    13561 cggtggtgtc ccggttttca gacgaccaac gcgcagcgga agttcacgca aacggcgaga
    13621 aagcggaatg gacggcggat caccagcacg gccaccgctg tagttatcca gaccgatatg
    13681 aattttaccg tcgaggaacg tcccaacggt gagcacgacg gctttggcac ggaacttcag
    13741 tcccatttgg gtaacagcac cgaccacacg atcgttttcg acaataagat cttcaaccgc
    13801 ctgctggaag atcatcaggt tcggttggtt ctccagcgcc gtacgtaccg cctgacggta
    13861 gagcacacga tccgcctgag ctcgggtagc gcgaactgcc ggtcccttgc ttgcgtttag
    13921 tatcctaaac tggatacccg cctgatcgat cgctttcgcc atcagaccgc cgagtgcatc
    13981 cacttctttt accagatgtc ccttcccaat accgccgatc gccgggttgc agctcatctg
    14041 ccccagagtg tcgatattgt gtgtcaaaag cagagtctgt tgacccatac gcgccgcagc
    14101 catcgcggcc tcggtgcctg catgaccccc gccaatgatg atgacgtcaa aaggatccgg
    14161 ataaaacatg gtgattgcct cgcataacgc ggtatgaaaa tggattgaag cccgggccgt
    14221 ggattctact caactttgtc ggcttgagaa agacctggga tcctgggtat taaaaagaag
    14281 atctatttat ttagagatct gttctattgt tatctcttat taggatcgca ctgccctgtg
    14341 gataacaagg atccggcttt taagatcaac aacctggaaa ggatcattaa ctgtgaatga
    14401 tcggtgatcc tggaccgtat aagctgggat cagaatgagg ggttatacac aactcaaaaa
    14461 ctgaacaacg gttgttcttt ggataactac cggttgatcc aagcttcctg acagagttat
    14521 ccacagtaga tcgcacgatc tgtatactta tttgagtaaa ttaatccacg atcccagcca
    14581 ttcttctgcc ggatcttccg gaatgtcgtg atcaagaatg ttgatcttca gtgtttcgcc
    14641 cgtctgtttt gcaccggaat ttttgagttc agcctcgagt ttatcgatag ccccacaaaa
    14701 tgtgtcatat tcacgactac caataccgat tgcgccaaag cggaccgcag aaagatcggg
    14761 cttctgttcc tgcaatgctt catagaaagg agaaaggttg tccggaatat ctccggcacc
    14821 gtgggtggag ctgataacca gccagatccc cgaggcagat aaatcttcca acagcggacc
    14881 gtgcagcgtt tcggtggtaa aacccgcctc ttccagcttt tcagccaggt gttctgctac
    14941 atattcggca ccgccgaggg tgctgccgct gataagagtt atatctgcca taaaccgcca
    15001 cctttattaa gagtggcgta ttgtacgctg tgaacgcgtt gggatctacc tgtggaaaag
    15061 tatgggatta aaaaagccga tcagggcttg atggtacgca tgatcgggtt ctgcaggacg
    15121 atcagtgtct cggtggactg aatttcatca attgtttgga tcttgttgat aagtacatgc
    15181 tggagagcat cgatcgaacg gcacatcact tttataaaga tgctgtagtg gccggtggtg
    15241 tagtacgcct cggtcacttc atcgaggctt tccagctttg ccagcgcgga agggtagtct
    15301 ttggcgctct ttaatataat gccgataaag cagcctacgt cataaccgag ctgcttcggg
    15361 ctgacatcaa tacgcgcccc ggtaatgatc cccgcctgct tcattttctc tactcgaacg
    15421 tgaatcgtcc ccggactgac gccaaattgt ttcgccagtt cggcgtaagc ggtgcgcgca
    15481 ttgcccatta atgcttccag gatgccacgg tccagattgt cgatcagata attttccata
    15541 ggattttctt atgcggattg atgattcatt ctattttagc cttctttttt aatgaatcaa
    15601 aagtgagtta ggctttttat tgaatgatta ttgcatgtgt gtcggttttt gttgcttaat
    15661 cataagcaac aggacgcagg agtataaaaa atgaaaaccg cttacattgc caaacaacgc
    15721 caaattagct tcgtgaaatc tcacttttct cgtcaactgg aagaacgtct ggggctgatc
    15781 gaagtccagg cgccgattct tagccgtgtg ggggatggca cgcaggataa cttgtcgggc
    15841 tgtgaaaaag cggtacaggt aaaagtgaaa gctctgcctg atgcccagtt cgaagtggtt
    15901 cattcactgg cgaagtggaa acgtcagacc ttagggcaac acgacttcag cgcgggcgaa
    15961 gggctgtaca cgcacatgaa agcccttcgc cccgatgaag accgtctttc tccgttgcac
    16021 tcggtctatg ttgaccagtg ggactgggaa cgcgtaatgg gcgacggtga gcgtcaattc
    16081 tcgactctga aaagcacggt agaggcgatt tgggcgggaa ttaaagcaac cgaagctgcg
    16141 gttagcgaag agtttggcct ggcaccgttc ctgccggatc agatccactt cgtacacagc
    16201 caggagttac tgtctcgtta tccggatctt gatgccaaag ggcgtgagcg ggcgatagcg
    16261 aaagatcttg gtgcggtatt ccttgtcggg attggcggca agctgagcga tggtcatcgc
    16321 cacgacgtgc gcgcaccgga ttatgatgac tggagcaccc cgtcagagct gggccatgcg
    16381 ggtctgaacg gcgatattct ggtgtggaac cctgtactgg aagatgcgtt tgagctttct
    16441 tccatgggga tccgcgtgga tgccgacacg ttgaagcatc agttggcgct gaccggtgat
    16501 gaagatcgtc tgcagctgga atggcatcag gcgttgctgc gcggtgaaat gccgcagacc
    16561 atcggcggcg gtatcggcca gtctcgtttg accatgctgc tgctgcaact gccgcatatc
    16621 ggccaggtcc agtgtggagt atggccagct gctgttcgcg agagcgtccc ttctctgctg
    16681 taataattta tcgccgccag cgtctgagca ggcggcttcg catcccggta tcaaagcgcc
    16741 agatatgatc gaaaatgcgc atgatgccgg gtttgccgtg tgccgacatc gccacggcat
    16801 gaaagcgatg ctgatgtacc cgctgcagct ctttcacttt actcgtcacg tcgtcaggca
    16861 accgctgggc gataaaatca gaaatcacca ccgcatcggc atcaaaccat tccctgcttt
    16921 gcaagcgttc cataatggcg cgaaaacaac tggcaagatc ggtgccgcca cgaaactgct
    16981 ggcttaaaaa acggattgct tgttcgatgc cttgtgggcc tgaaagctca taacggacga
    17041 tctcggtgga aaatagcata atataacagc gccggttttc agcgagagca atgcgcatca
    17101 aagccaggca gaacgctttc gcacactgtt cattaaagcc gcccattgag ccggaagtat
    17161 ccacgcagac aataaacggt ccgcgcggct gttcgtcgta atctttatgc accaccgggc
    17221 gttcgatcac tttttcacgc cacgactcac cgtgcaggcg ataggtgagc aactgttttt
    17281 ccaccagccg acggtaaaac tcatactcca gttccgttat ccctagtgtc gccagttctg
    17341 gcggcagaag acgtaaaata tcatcgcttt gttgcagacc atcaacctgc tcaggaaccg
    17401 tcgccggttc gcgcaccatg gtgcggaagg tttccatctg cgcatcgttg cgcggtattg
    17461 atttggcttc ccgggaacgc cccagttgtt ctgccaggcg tttcagttcc ggctgttcgt
    17521 taagaaattc accgtatttc acaatcaact gatagtcgcc acgtttaagc tgaccggcgc
    17581 tcatatccca cagacgacca gctgcggtat tgttatctgc gagaatcggt tcaagttgtc
    17641 cgctcagcgt catgcgttcc tgaacttcac tcaacagttg ttcgcgttct tcttctaata
    17701 gctgttgatt taacgttgtt gcttgcacga tcagacttaa acgccagcgc tggagaaaaa
    17761 gcgtgtgtaa cgctgaagtg atcgtgctgt tagcatcgac caactgtcgg gcttgttctg
    17821 cccatgggga attcagacga tgcagtaagt ccaggatctg tggtagctgc acaataaact
    17881 gcggcgtgga gaggagctgg ctttgctgat agcacatcac ctcttcggtg agttccggcg
    17941 ggactcgggc atctttcagc cgactgcgca gcgcctcacg ccagcgggga acatcatcag
    18001 tgattgccgc cttcagtcgt gggaattttt caaagaagac tgccagctgc ggtgaggcca
    18061 gcagcgcgat gatcatctct tcgatcaatc cctcttcgct gacggccagc atcacattaa
    18121 gcgtatccag cgttagcatt gttgtgcctg gcgaatctgt gcgccgacat cctgtaggct
    18181 ggcttcaatg cgacctaacc agtcgccagg gataaacaga catctttgct gttcgctgaa
    18241 taaagcgtgt tgcttgcgcc agtcgctttc aagctcttcc aattgttgtt ttatttcacc
    18301 tggcagacct tcagccgatg aaccgggaag tgccagcgta ctgccttgta aacttacatc
    18361 gcgtacaaca agatgttggg cgctatcaac ttccagattc agtttctggg caaagccgat
    18421 accgttcagt ttgccgcgaa tttcaccacc tttgctcagc cactgttcca gcgcgctacg
    18481 ctcaaaggag atatgaacca cttccatatt atgcaatttt aacggttttt gcagcagcag
    18541 agtcagagtg gaagcagtaa cgttaacagg aagttgatac tgctgacgac ggctgaaaat
    18601 gccgcccaga cgaattaccg ttaaggctgt tttatcgctt tgttgctgct ggagttgcag
    18661 gtgacgttgc acaatcgcgc ccaggcgggt caacatccct tgctgttgcc aggcatgacc
    18721 ggtcatcaat acatcaattt gttgttgtat caaattcagg ctttgcgcgt cataccacag
    18781 gcaatctttc agcaaaatga gatctaccgg ggcaacagca ctgcgaccgc taaaaaaggc
    18841 gctggcctgc aataatcgaa tcgctttttt ccagcgacga tccgagacat aaggcgcatc
    18901 cggtaattta tccagttgct ggcgcagcat aaaaatcagc tcaaatacat gatcgggcag
    18961 cgtaatttca ccaatctctt tttgccagcg ttcatattct tcatctgtga cctgcaagga
    19021 ggctggaacc ggattgtcat tttcatcctg ttgactggtc agcatggagc ggaaattcgc
    19081 tttatcctgc actttatcta accacagacg aatcagcatg cggtcatata acgcttccag
    19141 actgctgtct gcttccggca gctcgttgga ggccgccacc agcaggcgca tcgggatttt
    19201 ttctacgtgt gcaccgttgc ggaactggcg ctcgttaatg gcggtgagca gggtattaag
    19261 aattgccgga cccgctttcc agatctcatc cagaaagacg atttctgctt ccggcaggta
    19321 accgctggtt aaacgttcat agcgcccttc atcttttagc gcctgaatag aaaggggacc
    19381 aaaaacttct tccggcgtgg agaagcgggt catcagatat tcaaacgcgc gggcattctg
    19441 aaaggcgaat tttaagcgcc gggcgatcaa acttttggca atacctggcg ggccaaggag
    19501 gaacacactt tcaccactta atgccgctaa taaacacaag cggatggcgt ggctacgttc
    19561 ataaagcccc ttttccaacg aactgctcag gcgggaaatt ctttccgcta ataaatgagg
    19621 gtgagccata atgaagtggc gtcctttcgt caaaagttct gcgtaaattg cgagtataga
    19681 cgtttcttgc tggtggctaa aatagtctca aagggagggt atttttcttt gagccaggtt
    19741 aatgtggccg catttaggag tacgattttg ccgttaatcg tgcatactgt gcgctttttt
    19801 gtgggccaag ggactaagca cacatttcat atttcaacga aagactagtc tatgagcact
    19861 gataataagc aatcattgcc cgcgattacc ctcgcggcga ttggagttgt ctacggcgat
    19921 attggtacca gcccgttata tacacttcgt gaatgtttgt ccggccagtt tggttttggc
    19981 gttgaacgcg atgccgtgtt tggcttttta tcgctgatct tctggctgct aatctttgtg
    20041 gtttccatta aatatctcac cttcgtgatg cgggcagata acgccggtga aggggggatc
    20101 ctgacgttga tgtcgcttgc cgggcgtaat acgtcggcgc gaaccacatc aatgctggtg
    20161 attatggggc taatcggcgg cagctttttc tatggtgaag tcgtcataac acccgctatt
    20221 tcggtgatgt cagccattga aggtctggaa atcgtcgccc cgcagctgga tacctggata
    20281 gttcccctct caattatcgt tctcacttta ttatttatga ttcaaaagca tggcacggct
    20341 atggtcggta agctgtttgc gccgatcatg ctgacctggt ttttgattct ggcaggactg
    20401 gggttacgta gcattattgc taaccaggaa gtgctgtatg cgctgaatcc gatgtgggcg
    20461 gtgcatttct tccttgaata caaaacggtt tcttttattg cattaggggc agtggtgctg
    20521 tcgattacgg gggtcgaggc gctgtatgct gatatggggc actttggtaa gttccctatt
    20581 cgcctggcgt ggttcaccgt cgtattacct tccttgaccc ttaattactt cggtcaggga
    20641 gcgctgttgt taaagaatcc ggaagcgatt aagaacccgt tcttcctttt ggcaccggac
    20701 tgggcgctga tcccgctgct gatcatcgcc gcactggcga cggtaatcgc ctcgcaggcg
    20761 gttatctctg gcgtcttttc attgacgcgt caggcggtac gtctgggata tttgtcgccg
    20821 atgcgcatta ttcacacctc cgaaatggag tcagggcaaa tctatattcc gtttgtgaac
    20881 tggatgctct atgtcgcggt cgtgattgtg attgtcagct ttgagcactc cagcaacctg
    20941 gcggcggcgt acgggattgc ggtgaccgga accatggtgc tgacgtctat tctctcgact
    21001 accgtggcac gtcagaactg gcactggaat aagtattttg ttgcgctgat cttgattgct
    21061 ttcctttgtg tcgatattcc attgttcacc gctaacctcg ataaactgct ctccggcggc
    21121 tggttgccat tgagcctcgg tacggtgatg tttatcgtga tgaccacctg gaagagtgag
    21181 cgtttccgct tgctgcggcg gatgcatgaa catggtaact ctctggaagc gatgattgct
    21241 tcgctggaga aatcaccgcc tgttcgcgtg cccgggaccg cggtatatat gtcgcgtgca
    21301 atcaacgtca ttccctttgc gctgatgcat aaccttaaac ataacaaggt attgcatgag
    21361 cgggtgattc tgttaactct gcgcaccgaa gacgctccat atgtccataa cgtccgtcgg
    21421 gtacagattg aacaactgtc gcccactttc tggcgcgtgg tggcaagtta tggttggcga
    21481 gaaacgccaa acgtggaaga agttttccac cgctgcggtc tggaaggatt aagttgccgg
    21541 atgatggaaa cctccttctt tatgtcgcat gagtcgttga tcctcggcaa acgcccgtgg
    21601 tatttgcgtc tgcgcggcaa gctgtacttg ctgctgcaac gtaatgcgct gcgtgcacca
    21661 gatcaatttg aaatcccgcc aaacagggtt atcgaactgg gtactcaggt cgaaatctaa
    21721 cgccagacgc ctcctttctt cataaggggg cgtttttgtt ttcatggtta atcaccatgt
    21781 aaaacgtttc gaggttgatc acatttccgt aacgtcacga tggttttccc aactcagtca
    21841 ggattaaact gcgggtcagc gaaacgtttc gctgatggag aaaaaaatga aaaaaggcac
    21901 cgttcttaat tctgatattt catcggtgat ctcccgtctg ggacataccg atacgctggt
    21961 ggtgtgtgat gctggtttac ccatccccaa aagtacaacg cgtatcgata tggcattaac
    22021 ccagggtgta ccttctttta tgcaggtgct gggcgtcgtc acaaatgaaa tgcaggtcga
    22081 ggctgccatt atcgcggaag agatcaaaca ccataatccg caactccacg aaacgttgct
    22141 cactcacctt gagcagctgc aaaaacacca gggaaatacc attgaaattc gttacaccac
    22201 gcatgaacaa ttcaaacaac aaaccgcaga aagtcaggcg gtaattcgca gcggagaatg
    22261 ttctccgtat gcgaatatca ttctctgtgc tggcgtgacg ttctgaggcc gtcatggaag
    22321 cattacttca gcttaaaggc atcgataaag ccttcccggg cgtaaaagcc ctctcgggcg
    22381 cagcgttaaa tgtctatccg ggccgcgtga tggcgctggt gggcgaaaac ggcgcgggta
    22441 aatccaccat gatgaaagtg cttactggca tctatactcg cgatgccggt acgcttttat
    22501 ggctggggaa agaaacgaca tttaccgggc cgaaatcttc ccaggaagcc gggattggga
    22561 ttatccatca ggaactgaac ctgatcccgc agttaaccat tgccgaaaac attttcctcg
    22621 gtcgtgagtt tgttaatcgc tttggcaaaa ttgactggaa aaccatgtat gccgaagcgg
    22681 ataaattgct ggctaaactt aacctgcgct ttaaaagcga caagctggtg ggcgatcttt
    22741 ccatcggtga ccagcaaatg gttgaaatag ccaaagtgct gagctttgag tcgaaagtca
    22801 tcattatgga tgaaccgacc gatgcgctga ccgataccga aaccgaatcc ctgttccgcg
    22861 tcatccgcga gctgaaatcg caaggccgcg gtattgtcta tatctcccac cgcatgaaag
    22921 aaatcttcga gagttgcgat gacgttaccg tttttcgtga cggacaattt attgctgagc
    22981 gcgaagtggc atcactgacc gaagattcgc tgattgagat gatggtgggt cgcaagctgg
    23041 aagatcaata tccgcacctg gacaaagcgc cgggagatat ccgcctgaaa gtcgataatc
    23101 tctgcggacc tggcgttaac gatgtctctt ttactttacg caaaggcgaa attcttggcg
    23161 tctctggttt gatgggcgca ggtcgtaccg aactgatgaa agtgctctac ggcgcactac
    23221 cgcgcaccag cggttacgtc accctggatg ggcatgaagt cgttacccgt tcaccgcagg
    23281 atggcctggc aaacggcatt gtgtatatct ccgaagaccg taaacgtgac ggtttagtgt
    23341 tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct gcgctacttc agccgcgctg
    23401 gcggcagttt gaagcatgcc gatgaacagc aggctgtgag tgattttatt cgcctgttca
    23461 atgtgaaaac gccgtcgatg gagcaggcaa ttggtctgct ttccggtggc aatcagcaaa
    23521 aagtggcgat tgcccgcggt ctgatgacac gccccaaagt gttgatcctt gatgaaccta
    23581 cccgtggcgt agatgtcggc gcgaaaaaag agatctatca actgattaac cagttcaaag
    23641 ccgatggctt gagcatcatt ctggtgtcat cggagatgcc agaagtatta ggcatgagcg
    23701 atcgcatcat cgtcatgcat gaagggcatc tcagcgggga atttactcgt gagcaggcca
    23761 cccaggaagt gttaatggct gccgctgtgg gcaagcttaa tcgcgtgaat caggagtaaa
    23821 aaaatgacaa cccagactgt ctctggtcgc cgttatttca cgaaagcgtg gctgatggag
    23881 cagaaatcgc ttatcgctct gctggtgctg atcgcgattg tctcgacgtt aagcccgaac
    23941 tttttcacca tcaataactt attcaatatt ctccagcaaa cctcagtgaa cgccattatg
    24001 gcggtcggga tgacgctggt gatcctgacg tcgggcatcg acttgtcggt aggttctctg
    24061 ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca ttgaagtcaa tgcgctggtg
    24121 gctgtcgctg ccgctctcgc gttaggtgcc gcaattggtg cggtaaccgg agtgattgta
    24181 gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta tgatgctttt actgcgcggc
    24241 gtgaccatgg tttataccaa cggtagccca gtgaataccg gctttactga gaacgccgat
    24301 ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac cgacgccagt ctggatcatg
    24361 gggattgtct tcctcgcggc ctggtacatg ctgcatcaca cgcgtctggg gcgttacatc
    24421 tacgcgctgg gcggcaacga agcggcaacg cgtctttctg gtatcaacgt caataaaatc
    24481 aaaatcatcg tctattctct ttgtggtctg ctggcatcac tggccgggat cattgaagtg
    24541 gcgcgtctct cctccgcaca acccacggcg gggactggct atgagctgga tgctattgct
    24601 gcggtggttc tgggcggtac gagtctggcg ggcggaaaag gtcgcattgt tgggacgttg
    24661 atcggcgcat taattcttgg cttccttaat aatggattga atttgttagg tgtttcctcc
    24721 tattaccaga tgatcgtcaa agcggtggtg attttgctgg cggtgctggt agacaacaaa
    24781 aagcagtaat aacgactaca ggacatcttg aatatgaaca tgaaaaaact ggctaccctg
    24841 gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg cgatggcaaa agacaccatc
    24901 gcgctggtgg tctccacgct taacaacccg ttctttgtat cgctgaaaga tggcgcgcag
    24961 aaagaggcgg ataaacttgg ctataacctg gtggtgctgg actcccagaa caacccggcg
    25021 aaagagctgg cgaacgtgca ggacttaacc gttcgcggca ccaaaattct gctgattaac
    25081 ccgaccgact ccgacgcagt aggtaatgct gtgaagatgg ctaaccaagc gaacatcccg
    25141 gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg tgagccacat tgcttctgat
    25201 aacgtactgg gtggcaaaat cgccggtgat tacatcgcga agaaagcggg tgaaggtgcc
    25261 aaagttatcg agctgcaagg cattgctggt acatccgcag cccgtgaacg tggcgaaggc
    25321 ttccagcagg ccgttgctgc tcacaagttt aatgttcttg ccagccagcc agcagatttt
    25381 gatcgcacca aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc ggatgttcag
    25441 gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcgcgcact gcaaactgcc
    25501 ggtaaatcgg atgtgatggt cgtcggattt gacggtacac cggatggcga aaaagcggtg
    25561 aatgatggca aactagcagc gactatcgct cagctacccg atcagattgg cgcgaaaggc
    25621 gttgaaaccg cagataaagt gctgaaaggc gagaaagttc aggctaagta tccggttgat
    25681 ctgaaactgg ttgttaagca gtagttttaa tcaggttgta tgacctgatg gtgacataaa
    25741 tacgtcatcg acagatgaac gtgtaatata aagaaaagca gggcacgcgc caccctaaca
    25801 cggtggcgca ttttatggac atcccgaata tgcaaaacgc aggcagcctc gttgttcttg
    25861 gcagcattaa tgctgaccac attcttaatc ttcaatcttt tcctactcca ggcgaaaccg
    25921 taaccggtaa ccactatcag gttgcatttg gcggcaaagg cgcgaatcag gctgtggctg
    25981 ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac tggcgatgac agcattggcg
    26041 agagcgttcg ccagcagctc gccactgata acatcgatat tactccggtc agcgtgatca
    26101 aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg cgaaggtgag aatgtcatcg
    26161 gtattcatgc cggcgctaat gctgcccttt ccccggcgct ggtggaagcg caacgtgagc
    26221 gtattgccaa cgcgtcagca ttattaatgc agctggaatc accactcgaa agtgtgatgg
    26281 cagcggcgaa aatcgcccat caaaataaga ctatcgttgc gcttaacccg gctccggctc
    26341 gcgaacttcc tgacgaactg ctggcgctgg tggacattat tacgccaaac gaaacggaag
    26401 cagaaaagct caccggtatt cgtgttgaaa atgatgaaga tgcagcgaag gcggcgcagg
    26461 tactgcatga aaaaggtatc cgtactgtac tgattacttt aggaagtcgt ggtgtatggg
    26521 ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg ggtgcaggct gtcgatacca
    26581 ttgctgccgg agataccttt aacggcgcgt taatcacggc gttgctggaa gaaaaaccat
    26641 tgccagaggc gattcggttt gcccatgctg ccgctgcgat tgccgtaaca cgtaaaggcg
    26701 cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt tttagacagg cagaggtgac
    26761 gcttggctac aatgaaagat gttgcccgcc tggcgggcgt ttctacctca acagtttctc
    26821 acgttatcaa taaagatcgc ttcgtcagtg aagcgattac cgccaaagtt gaagcggcga
    26881 ttaaagaact caattacgcg ccatcagctc tggcgcgtag cctcaaactc aatcaaacac
    26941 ataccattgg catgttgatc actgccagta ccaatccttt ctattcagaa ctggtgcgtg
    27001 gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt cctttgcaat accgaaggcg
    27061 atgaacagcg gatgaatcgc aatctggaaa cgctgatgca aaaacgcgtt gatggcttgc
    27121 tgttactgtg caccgaaacg catcaacctt cgcgtgaaat catgcaacgt tatccgacag
    27181 tgcctactgt gatgatggac tgggctccgt tcgatggcga cagcgatctt attcaggata
    27241 actcgttgct gggcggagac ttagcaacgc aatatctgat cgataaaggt catacccgta
    27301 tcgcctgtat taccggcccg ctggataaaa ctccggcgcg cctgcggttg gaaggttatc
    27361 gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg ctatgaagtc actggtgatt
    27421 ttgaatttaa cggcgggttt gacgctatgc gccaactgct atcacatccg ctgcgtcctc
    27481 aggccgtctt taccggaaat gacgctatgg ctgttggcgt ttaccaggcg ttatatcagg
    27541 cagagttaca ggttccgcag gatatcgcgg tgattggcta tgacgatatc gaactggcaa
    27601 gctttatgac gccaccatta accacgatcc atcaaccaaa agatgaactg ggggagctgg
    27661 cgattgatgt actcatccat cggataaccc agccgaccct tcagcaacaa cgattacaac
    27721 ttactccgat tctgatggaa cgtggttcgg cttagattta cgctgtcttt tgatcaaatt
    27781 attaccatcg gttgttttca gaagcatgaa cattgctgct gaagcaacag taataatgcc
    27841 cattgtgata aacgtatagt ggaattgttc gacagtcgtt gtgccttcca ttccttcata
    27901 aacgcgaagg acggccgcac ttacagctac gcctaaactg atagacagtt gctgcgtgac
    27961 cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc agatcggcaa gtgtgatggt
    28021 attcatcgcg gtaaactgcg tcgacatagc catccctaat ataaacaacg gcaagatcag
    28081 catccatatt gccattgccg gtgattgcaa agagaactga gcgatcatta gcccaataat
    28141 caccgtgatc cccactaatg tatggcgata gcccagacga cgtaagactt gggtaaccat
    28201 cgattttgca ataatggaac ctaacgctgt cggtgccatc atacagccag caataaacgc
    28261 ctgataacca aatcctacct gtaacattaa tggcataagg aacggtacac agccggtccc
    28321 cagacgggtt gcaatattgc ctacgatacc gatcgagaaa gtgcgggttt taaataaatc
    28381 taatgaaatt aatgggtttg gcgtgcgtcg tgcatggaga atatagagaa gcagtaaccc
    28441 gatgctggtg acaattaccg tcaaggcaat ccagctggcg acaatctttt ccccgaatag
    28501 ctctattccg cttgagaaga gaacaaggct gaggccaaac agcaaaaagc cagtgatatc
    28561 gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg cgcgcgtaaa gaaggcccgc
    28621 aatacctatg gggatattga ttaaaaatat ccagtgccag gttgcccagg tgaccagcac
    28681 gccgccaaga atggggccta aaattggccc caccagaccc ggcatggcga caaaattcaa
    28741 tactggaaga agttcattac gaggataagc gcgcagtaag gccagccgag caacaggcat
    28801 catcattgcg ccgcctatcc cctgaataac ccggaagaca accagctgtg gtagcgaatt
    28861 agaaagtgcg caggccagag aacccaatgt gaacagactc acggcaaggg taaaaatgcg
    28921 acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga ataagcatcg ccaccgtcag
    28981 cgtataactg atgatggctg attgcatcgc gagaggagaa cgattaaggc tatgagcgat
    29041 tgcgggtaag gcggtattca gaatagtggc atcaagtgcc tgcatgaaga aggccatcgc
    29101 cgcgatccac ggcaaacccg ccatactgcg cttcttttta tcgctcattc aatgtcctgt
    29161 tatcgggtta tcacttatca ggtgagcgta gcagcgcctg acaagcttta aatgccgcgt
    29221 cgccatcgct ttggataatc gcatcgacta tcgcctgatg cagatccagc tttatcactg
    29281 tgtcgctggt aattgacgtg aagtaagtgt gataaaccga atggaatagc gaggcgaatg
    29341 aggtcaaaaa cggattggcg ctcatttcat agatatgctc atgccaggcc atatcgactt
    29401 cgatccagcg ttcacggcga aagttctctt ttaatgccgc catttcggcc attaacgtat
    29461 tgagatgcgc tttctgttcc gcggtgccaa ccgttgctgc cagtaggcaa gcttgtggct
    29521 ccagacagat acgcataacc agaaagtgat cgatgacctg atgaaagttc tcttctgtca
    29581 tccaccaggt aagcaattcc tgatcaagaa aattccagtt cgattgtggc atgacccggg
    29641 taccaattcg cggtcgcggt aaaaccatcc cttttgccgt taacgttttg accgcttcgc
    29701 gtaccgctgt acgactcact ccaaattgct cgcccagctc aatctcaccg ggcaaaatgg
    29761 tgccgggttc atattcacct tttaagatcc gttgcgccag cttctcagcc agaacatacg
    29821 aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg cattactaat cttccttttt
    29881 ctttttattc ctccttagta tgccaccagg aagtgtgatt acggttgcaa aaacggcaaa
    29941 ttgcttgttt tatggcacat taacggggct tttgctgaaa aaatgcgcgg tcagaaaatt
    30001 attttaaa
//
LOCUS       DAZKIF010000053        29414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZKIF010000053 DAZKIF010000000
VERSION     DAZKIF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.053"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..286)
                     /locus_tag="ACHOTW_002298"
     CDS             complement(<1..286)
                     /locus_tag="ACHOTW_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFP9844405.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRD"
     gene            complement(894..2261)
                     /gene="rhlE"
                     /locus_tag="ACHOTW_002299"
     CDS             complement(894..2261)
                     /gene="rhlE"
                     /locus_tag="ACHOTW_002299"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP9844406.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            2490..3161
                     /gene="cecR"
                     /locus_tag="ACHOTW_002300"
     CDS             2490..3161
                     /gene="cecR"
                     /locus_tag="ACHOTW_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP9844407.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            3164..4159
                     /gene="hlyD"
                     /locus_tag="ACHOTW_002301"
     CDS             3164..4159
                     /gene="hlyD"
                     /locus_tag="ACHOTW_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP9844408.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            4152..5888
                     /gene="ybhF"
                     /locus_tag="ACHOTW_002302"
     CDS             4152..5888
                     /gene="ybhF"
                     /locus_tag="ACHOTW_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9844409.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5881..7014
                     /gene="ybhS"
                     /locus_tag="ACHOTW_002303"
     CDS             5881..7014
                     /gene="ybhS"
                     /locus_tag="ACHOTW_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9844410.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            7025..8131
                     /gene="ybhR"
                     /locus_tag="ACHOTW_002304"
     CDS             7025..8131
                     /gene="ybhR"
                     /locus_tag="ACHOTW_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9844411.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(8093..8503)
                     /gene="ybhQ"
                     /locus_tag="ACHOTW_002305"
     CDS             complement(8093..8503)
                     /gene="ybhQ"
                     /locus_tag="ACHOTW_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP9844412.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            8636..9397
                     /gene="ybhP"
                     /locus_tag="ACHOTW_002306"
     CDS             8636..9397
                     /gene="ybhP"
                     /locus_tag="ACHOTW_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9844413.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            9394..10635
                     /gene="clsB"
                     /locus_tag="ACHOTW_002307"
     CDS             9394..10635
                     /gene="clsB"
                     /locus_tag="ACHOTW_002307"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP9844414.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            10635..11591
                     /gene="ybhN"
                     /locus_tag="ACHOTW_002308"
     CDS             10635..11591
                     /gene="ybhN"
                     /locus_tag="ACHOTW_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP9844415.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLAHFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(11627..12340)
                     /gene="ybhM"
                     /locus_tag="ACHOTW_002309"
     CDS             complement(11627..12340)
                     /gene="ybhM"
                     /locus_tag="ACHOTW_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9844416.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(12548..13252)
                     /gene="ybhL"
                     /locus_tag="ACHOTW_002310"
     CDS             complement(12548..13252)
                     /gene="ybhL"
                     /locus_tag="ACHOTW_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9844417.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(13388..13840)
                     /gene="moaE"
                     /locus_tag="ACHOTW_002311"
     CDS             complement(13388..13840)
                     /gene="moaE"
                     /locus_tag="ACHOTW_002311"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP9844418.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGLVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13842..14087)
                     /gene="moaD"
                     /locus_tag="ACHOTW_002312"
     CDS             complement(13842..14087)
                     /gene="moaD"
                     /locus_tag="ACHOTW_002312"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP9844419.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(14080..14565)
                     /gene="moaC"
                     /locus_tag="ACHOTW_002313"
     CDS             complement(14080..14565)
                     /gene="moaC"
                     /locus_tag="ACHOTW_002313"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP9844420.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(14568..15080)
                     /gene="moaB"
                     /locus_tag="ACHOTW_002314"
     CDS             complement(14568..15080)
                     /gene="moaB"
                     /locus_tag="ACHOTW_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP9844421.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(15102..16091)
                     /gene="moaA"
                     /locus_tag="ACHOTW_002315"
     CDS             complement(15102..16091)
                     /gene="moaA"
                     /locus_tag="ACHOTW_002315"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP9844422.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(16079..16223)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            16488..17396
                     /gene="yvcK"
                     /locus_tag="ACHOTW_002316"
     CDS             16488..17396
                     /gene="yvcK"
                     /locus_tag="ACHOTW_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9844423.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(17587..19608)
                     /gene="uvrB"
                     /locus_tag="ACHOTW_002317"
     CDS             complement(17587..19608)
                     /gene="uvrB"
                     /locus_tag="ACHOTW_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9844424.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(20187..20864)
                     /gene="bioD"
                     /locus_tag="ACHOTW_002318"
     CDS             complement(20187..20864)
                     /gene="bioD"
                     /locus_tag="ACHOTW_002318"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9844425.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGESPWLAENPENAATGK
                     YINLALL"
     gene            complement(20857..21612)
                     /gene="bioC"
                     /locus_tag="ACHOTW_002319"
     CDS             complement(20857..21612)
                     /gene="bioC"
                     /locus_tag="ACHOTW_002319"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP9844426.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(21599..22753)
                     /gene="bioF"
                     /locus_tag="ACHOTW_002320"
     CDS             complement(21599..22753)
                     /gene="bioF"
                     /locus_tag="ACHOTW_002320"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP9844427.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(22750..23790)
                     /gene="bioB"
                     /locus_tag="ACHOTW_002321"
     CDS             complement(22750..23790)
                     /gene="bioB"
                     /locus_tag="ACHOTW_002321"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP9844428.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23877..25166
                     /gene="bioA"
                     /locus_tag="ACHOTW_002322"
     CDS             23877..25166
                     /gene="bioA"
                     /locus_tag="ACHOTW_002322"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP9844429.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            25225..25461
                     /locus_tag="ACHOTW_002323"
                     /pseudo
     CDS             25225..25461
                     /locus_tag="ACHOTW_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            25516..25608
                     /locus_tag="ACHOTW_002324"
                     /pseudo
     CDS             25516..25608
                     /locus_tag="ACHOTW_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25705..26403)
                     /gene="nleD"
                     /locus_tag="ACHOTW_002325"
     CDS             complement(25705..26403)
                     /gene="nleD"
                     /locus_tag="ACHOTW_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFP9844430.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(26465..26586)
                     /locus_tag="ACHOTW_002326"
                     /pseudo
     CDS             complement(26465..26586)
                     /locus_tag="ACHOTW_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(26634..27515)
                     /gene="nleH1-1"
                     /locus_tag="ACHOTW_002327"
     CDS             complement(26634..27515)
                     /gene="nleH1-1"
                     /locus_tag="ACHOTW_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HFP9844431.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFVDTTETNVLYDRAKNEFNPIDISSYNVSDRSWSESQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(27685..27840)
                     /locus_tag="ACHOTW_002328"
                     /pseudo
     CDS             complement(27685..27840)
                     /locus_tag="ACHOTW_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            28119..28223
                     /locus_tag="ACHOTW_002329"
                     /pseudo
     CDS             28119..28223
                     /locus_tag="ACHOTW_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(28343..29362)
                     /gene="nleC"
                     /locus_tag="ACHOTW_002330"
     CDS             complement(28343..29362)
                     /gene="nleC"
                     /locus_tag="ACHOTW_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFP9844432.1"
                     /translation="MICRVLDINMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEKVHIGCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEA
                     GENDNEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVD
                     KHNQQ"
BASE COUNT         7120 a         7700 c         7330 g         7264 t
ORIGIN      
        1 catcacgagg aattgcatca ttcatattta tagagatcac attttcatgt ctggattgcc
       61 cggttttaat cgtcatattg atatcatttg cgctgatctc cgaaatctca ctgacgaatt
      121 ttttaaatgc attatagcgc tggtggccag cattaaattt gccaaatgaa acataatatg
      181 cgaaatgttc aaggaaacca tgtaattcca gtaaattaac cgggtcagaa ccctgcacca
      241 tatttaataa catcgtcgca taaccattgc attttgaaca atccatacat cgctcctttg
      301 cacttgaatg atctgcgaat cataagcctg gcaaaatggg gagtcttaca gaaatcgctc
      361 atttttaact tttcgtactg caaattcagg atcaacctgg ttatgaaagt ttgctgctgt
      421 acggctctaa acagactggc aatcaatctt tctatcaata agctgaaatg agcgattttt
      481 gatagtttat tagaactact aaagatatac ggtaatgaaa cgactgcaca ctttattttg
      541 cttagggaga tagtcggata cactatagac aatgaaaata ataaaatcag ggacagaatc
      601 aagctaatac tgaatcacat atctcataat aatttaccag atgatgaaca aactctttat
      661 gcaatagcat gtttattcca gattttccca gaatttgttt atgaatatca accaatgtta
      721 ttaacaataa ttcaaattgc aaggagaaat aatttcattg ccattttaga tacactagat
      781 agatcataaa gcaaatttat taaaacaata aaatcagaca tgtcatgcat gtctgaccgt
      841 gttacctgaa taatgggcaa aagcctgtga tactcttttg cccttacatg gtattactgc
      901 gcagcggcag gtttacgcgg acggcgacgg cgttgttgtt cgcctgccgg tttggcatcg
      961 ccaagacgac gagatggttt ttctgcaggc ttcgcgctag ctgatttcgc accaccttct
     1021 ccacggcgtg gttgctgttg accgcgacca ccaccacctt gcccacgacc gccgccgcca
     1081 cgttgctggc gaccgttctg gatcggttcg gctttgattg acgggtccgg ctcataaccc
     1141 ggaatcgcaa tgcgcgggat ctcttttttc agcagttttt cgatatcacg cagcagtttg
     1201 tgttcatcaa cacacaccag cgacaatgct tcgccagttg ccgccgcacg gccagtacgc
     1261 ccgatacggt ggacataatc ttccggtacg tttggcagtt catagttgac gacgtgcggc
     1321 aactcttcga tatccagacc gcgcgcagcg atgtcagttg ccaccagtac acgaatatcg
     1381 cccgatttaa aatcggccag cgcacgggta cgcgcacctt gcgatttatt accgtggatc
     1441 gccgcactac ggatgccatc tttattaagc tgttcagcga gatggttagc gccatgtttg
     1501 gtacgggtaa acaccagcac ctgctgccag ttccctttcc caatcatgtg cgacagcaat
     1561 tcgcgcttgc gtttcttatc aacaaagtga acatgctggg tcacctgatc agacgcggta
     1621 ttgcggcgtg ccacttctat ttccagcggg ttgtgcaaca gtttttccgc cagggcttta
     1681 atatcgtcag agaaggtcgc ggagaacaac aggttctggc gcttcgcagg tagttttgtt
     1741 aacacgcgac ggatatcgtg gataaagccc atgtcgagca tgcggtcagc ttcatcgagg
     1801 acgaggattt caacctgatc cagcttcact gcattctgat gttccaggtc cagcaaacgt
     1861 cccggcgttg ccaccagcac atcaacgccg ccgcgcaact tcatcatttg tgggttaatg
     1921 ctgacaccgc caaacaccac cagcgaacga atgttcaggt atttgctgta atcacggacg
     1981 ttttcgccaa tctgcgccgc cagttcacgg gtcggggtaa gaatgagcgc acgtaccgga
     2041 cgacgccctt tggcgtgcgg ctggcgagtg atcaggtgtt gcaacagcgg cagcgtaaag
     2101 cccgctgttt tgccggtgcc ggtctgggcg ctagccatca ggtcgcggcc ttccagcacc
     2161 gcagggatcg cctgctgctg aataggggtg ggttcacggt aaccctgctc ggcaacggcg
     2221 cgcaggatat cagggcttaa acccaaagaa tcgaaagaca taactactcc gaaccgcccc
     2281 gaccgttacc ggtgtagttt tcagggagat actgaaaagg aaaatgacaa aaaccacaat
     2341 gtcatgaggg agcggagtgt agcagttttt gtgacgcagc gcataaatta tccctgtgac
     2401 tggcgtagct aaaattctta gccatactgg acaactccca tatggagtca taatcttaat
     2461 caatcatttg attaagaatg gactgtgcga tgaataatcc tgccatgaca atcaagggtg
     2521 aacaggcgaa aaaacagctg attgctgccg cactggcgca gtttggtgaa tatggaatga
     2581 acgccaccac ccgcgagata gccgcccagg ccgggcagaa tatcgctgcc atcacctact
     2641 acttcggttc gaaagaagat ttgtacctcg cctgcgccca gtggattgcc gattttattg
     2701 gcgagcagtt ccgtccgcat gccgaggaag ccgaacgctt gttcgcccaa ccacagcctg
     2761 atcgggctgc catccgtgaa ctgatccttc gcgcctgcag gaacatgatt aagctgctca
     2821 cccaggatga caccgtcaac ctcagcaagt ttatctcccg tgagcagctc tctcccacgg
     2881 cagcctacca cctggtgcac gaacaggtga ttagtccgct acacagccac ctgacacgcc
     2941 tgattgccgc ctggaccggc tgcgacgcca atgacacccg catgatcctt catacccatg
     3001 cgttgattgg tgagattctg gcgttccgtc ttggcaaaga aacaattctg ttacgtaccg
     3061 gctggaccgc gttcgatgaa gaaaagaccg aactgatcaa ccagacggtg acctgtcata
     3121 tcgacctgat tttgcaagga ttatcgcaaa ggagtttgta gtgatgaaaa aacctgtcgt
     3181 gatcggactg gcggtagtgg tacttgccgc cgtggttgcc ggaggctact ggtggtatca
     3241 aagccgccag gataacggcc tgacgctgta tggcaacgtg gatattcgta cggtgaatct
     3301 tagtttccgt gttggggggc gcgttgaatc gctggcggtg gacgaaggtg atgctatcaa
     3361 agcgggccag gtgctgggcg aactggatca caagccgtat gagattgccc tgatgcaggc
     3421 gaaagcgggc gtctcggtgg cacaggcgca gtatgacctg atgcttgccg ggtatcgcga
     3481 tgaagaaatc gctcaggccg ccgcagcggt gaaacaggcg caagccgcct atgactatgc
     3541 gcagaacttc tataaccgcc agcaaggact gtggaaaagc cgcaccattt cggcaaatga
     3601 cctggaaaat gcccgctcct cgcgcgacca ggcacaggca acgctgaaat cagcacagga
     3661 taaattgcgt cagtaccgtt ccggtaaccg tgaacaggac atcgctcagg cgaaagccag
     3721 cctcgaacag gcgcaggcgc aactggcgca ggcggagctg aatttacagg actcaacgtt
     3781 gatagcaccg tctgatggca cgctgttaac gcgcgcggtg gagccaggca cggttctcaa
     3841 tgaaggtggc acggtgttta ccgtttcact aacgcgtccg gtgtgggtgc gtgcttatgt
     3901 tgatgaacgg aatcttgacc aggcccagcc ggggcgcaaa gtgctgcttt ataccgatgg
     3961 tcgcccggac aagccctatc acgggcagat tggtttcgtt tcgccgactg ctgaatttac
     4021 cccgaaaacc gtcgaaacac cggatctgcg taccgacctc gtctatcgcc tgcgtattgt
     4081 ggtgaccgac gccgatgatg cgttacgcca gggaatgcca gtgacggtac aattcggtga
     4141 cgaggcagga catgaatgat gccgttatca cgctgaacgg cctggaaaaa cgctttccgg
     4201 gcatggacaa gcccgccgtc gcgccgctcg attgcaccat tcatgccggt tatgtgacgg
     4261 ggctggtagg gccagacggt gcgggtaaaa ccacgctgat gcggatgctg gcgggattac
     4321 tgaaacccga cagcggcaat gccacggtga ttggctttga tccgatcaaa aacgacggcg
     4381 cgctgcacgc cgtgctcggc tatatgccgc agaaattcgg cctgtatgaa gatctgacgg
     4441 tgatggagaa cctcaatctg tacgcggatt tgcgcagcgt caccggcgag gcacggaagc
     4501 agacttttgc tcgcctgctg gagtttacgt ctcttggccc gtttaccgga cgcctggcgg
     4561 gcaagctctc cggtgggatg aaacaaaaac tcggcctggc ctgtaccctg gtgggcgaac
     4621 cgaaagtgtt gctgctcgat gaacccggcg tcggcgttga ccctatctca cggcgcgaac
     4681 tgtggcagat ggtgcatgag ctggcgggcg aagggatgtt aatcctctgg agtacctcgt
     4741 atctcgacga agccgagcag tgccgtgacg tgttactgat gaacgaaggc gagttgctgt
     4801 atcagggaga accaaaagcc ctgacacaaa ccatggccgg acgcagcttt ctgatgacca
     4861 gtccacacga gggcaaccgc aaactgttgc aacgcgcctt gaaactgccg caggtcagcg
     4921 acggcatgat tcaggggaaa tcggtacgtc tgatcctcaa aaaagaggcc acaccagacg
     4981 atattcgcca tgccgacggg atgccggaaa tcaacatcaa cgaaactacg ccgcgttttg
     5041 aagatgcgtt tattgatttg ctgggcggtg ccggaacctc agaatcgccg ctgggtgcaa
     5101 tattgcatac ggtagaaggc acacccggcg agacggtgat cgaagcgaaa gaactgacca
     5161 agaaatttgg tgattttgcc gccaccgatc acgtcaactt tgccgttaaa cgcggggaga
     5221 tttttggttt gctggggcca aacggcgcgg gcaaatcgac cacctttaag atgatgtgcg
     5281 gtttgctggt gccgacttcc ggccaggcgc tggtgctggg gatggatctg aaagagagtt
     5341 ccggtaaagc gcgccagcat ctcggctata tggcgcaaaa attttcgctc tacggcaacc
     5401 tgacggtcga acagaattta cgctttttct ctggtgtgta tggcttacgc ggtcgggcgc
     5461 agaacgaaaa aatctcccgt atgagcgagg catttggcct gaaaagtatt gcctcacacg
     5521 ccaccgatga actgccatta ggttttaaac agcggctggc gctggcctgt tcgctgatgc
     5581 atgaaccgga tattctgttt ctcgacgaac cgacttccgg cgttgacccc ctcacccgcc
     5641 gtgaattctg gctgcacatc aacagcatgg tagagaaagg cgtcacggtg atggtcacca
     5701 cccactttat ggatgaagcg gaatattgcg accgcatcgg cctggtgtac cgcgggaaat
     5761 taatcgccag cggcacgccg gacgatttga aagcacagtc ggctaacgat gagcaacccg
     5821 atcccaccat ggagcaagcc tttattcagt tgatccacga ctgggataag gagcatagca
     5881 atgagtaacc cgatcctttc ctggcgtcgc gtgcgggcgc tgtgcgtcaa agagacgcgg
     5941 cagatcgttc gcgatccgag tagctggctg attgcggtag tgatcccgct gttgctgctg
     6001 tttatttttg gttatggcat taacctcgac tccagcaagc tgcgggtcgg gattttactg
     6061 gaacagcgta gcgaagcagc gctggatttc acccacacca tgaccggttc gccctacatc
     6121 gacgccacca tcagcgataa ccgtcaggaa ctgatcgcca aaatgcaggc ggggaaaatt
     6181 cgcggtctgg tggttattcc cgtggatttc gccgaacaga tggagcgcgc caacgctacc
     6241 gcaccgattc aggtgatcac cgacggcagt gagccgaata ccgctaactt tgtgcaggga
     6301 tatgtcgaag ggatctggca gatctggcaa atgcagcggg cggaggataa cgggcagacc
     6361 tttgagccgc ttattgatgt acaaacccgc tactggttta acccggcggc gattagccag
     6421 cactttatta tccccggtgc ggtgaccatc atcatgacgg tcatcggcgc gattctcacc
     6481 tcgctggtgg tggcgcgaga atgggaacgc ggcactatgg aggctctgct ctctacggag
     6541 attacccgca cggaactgct gctgtgtaag ctgatccctt attactttct cgggatgctg
     6601 gcgatgttgc tgtgtatgct ggtgtcagtg tttattctcg gcgtgccgta tcgcggttcg
     6661 ctgctgattc tgttttttat ctccagcctg tttttactca gtaccctggg gatggggctg
     6721 ctgatttcca cgattacccg caaccagttc aatgccgctc aggtcgccct gaacgccgcg
     6781 ttcctgccgt cgattatgct ttccggcttt atttttcaga tcgacagtat gcccgcggtg
     6841 atccgcgcgg tgacgtacat tattcccgca cgttatttcg tcagcaccct gcaaagcctg
     6901 ttcctcgccg ggaatattcc agtggtgctg gtggtgaacg tactgttttt gatcgcttcg
     6961 gcggtgatgt ttatcggcct gacgtggctg aaaaccaaac gtcggctgga ttagggagaa
     7021 gagcatgttt catcgcttat ggacgttaat ccgcaaagag ttgcagtcgt tgctgcgcga
     7081 accacaaacc cgcgcgattc tgattttacc ggtgctgatt caggtgattc tgttcccgtt
     7141 cgccgccacg ctggaagtga ccaacgccac catcgccatc tacgatgaag ataacggcga
     7201 gcattcggtg gagctgaccc aacgttttgc ccgcgccagc gcctttacac atgtgctgct
     7261 gctgaaaagc ccacaggaga tccgcccgac catcgacaca caaaaggcgt tactactggt
     7321 gcgtttcccg gctgacttct cgcgcaaact ggataccttc cagaccgcgc cgttgcagtt
     7381 gatcctcgac gggcgtaact ccaacagtgc gcaaattgcc gccaactacc tgcaacagat
     7441 cgtcaaaaat tatcagcagg agctgctgga aggaaaaccg aaacctaaca acagcgagct
     7501 ggtggtacgc aactggtata acccgaatct cgactacaaa tggtttgtgg tgccgtcgct
     7561 gatcgccatg atcaccacta ttggcgtaat gatcgtcact tcactttccg tcgcccgcga
     7621 acgtgaacaa ggtacgctcg atcagctact ggtttcgccg ctcaccacct ggcagatctt
     7681 catcggcaaa gccgtaccgg cgttaattgt cgccaccttc caggccacca ttgtgctggc
     7741 gattggtatc tgggcgtatc aaatcccctt cgccggatcg ctggcgctgt tctactttac
     7801 gatggtgatt tatggtttat cgctggtggg attcggtctg ttgatttcat cactctgttc
     7861 aacacaacag caggcgttta tcggcgtgtt tgtctttatg atgcccgcca ttctcctttc
     7921 cggttacgtt tcgccggtgg aaaacatgcc agtatggctg caaaacctga cgtggattaa
     7981 tcctattcgc cactttacgg acattaccaa gcagatttat ttgaaggatg cgagtctgga
     8041 tattgtgtgg aatagtttgt ggccgctact ggtgataacg gccacgacag ggtcagcggc
     8101 gtacgcgatg tttagacgta aggtgatgta acttcttatc tttcgccagc aaagacacta
     8161 ccgccgggcc ggcaaggatt gccagccctg caattgccag caaaaagttg ttttccagta
     8221 cgcgggacaa cagccacagc acaatcagcg ctgcgccaaa ataccaggtc gtggtcagct
     8281 cttccagcac gtctgccacc tcacgccagc gttgctcggg gtggcgctga aacaccagca
     8341 tcatcactac cgtgacacaa tacagtgcgc ataatcccac gccgacgtga accagagtct
     8401 tactcatcca gcccaccacc agcagcgcca ctaccagtaa atgcaacata atctgccagc
     8461 aacttagacc cgttgcgaca cgaacacgtt gttgccactt catggcttct ctcctgaaga
     8521 atttatctct acttactcag agtaccgaac tttacggaaa attccgtaag accgcacctt
     8581 tttgtgacgc cgactacact atttcaatga gataagaggc aaagacagga aaattatgcc
     8641 cgatcaaaca caacaatttt cgttcaaggt gctcaccatc aatattcaca aaggctttac
     8701 cgcgtttaac cgacgcttca ttttgccgga acttcgcgat gccgtgcgta ccgtcagcgc
     8761 cgatattgtt tgcctgcagg aagtgatggg cgcgcacgaa gttcatccgc tgcatgtgga
     8821 aaactggccc gatacctcgc actacgagtt tctcgccgac actatgtgga gcgattttgc
     8881 ctacggtcgc aatgccgtat acccggaagg gcatcacggc aacgccgtac tgtcgcgtta
     8941 tcccattgaa cattatgaga atcgcgatgt ttcggtcgat ggtgcggaaa agcgcggcgt
     9001 gctctactgc cgcattgtgc cgccgatgac cggaaaagcg attcatgtga tgtgcgtaca
     9061 tctgggcctg cgtgaggcgc accgtcaggc gcagcttgcg atgctcgccg aatgggtgaa
     9121 tgagctaccg gacggcgaac cggtattggt ggcgggtgat ttcaatgact ggcggcaaaa
     9181 agctaatcat ccgttaaaag tgcaggccgg actggatgag atttttaccc gcgcccacgg
     9241 acgcccggcg cgcacgtttc cggtgcaatt tcctctacta cgactggaca ggatctacgt
     9301 caaaaatgcc agcgccagcg cgccaaccgc gttgccgctg cggacatggc gacacctttc
     9361 tgatcatgcc cctttaagtg cggagattca tttatgaaat gtagctggcg cgaaggcaat
     9421 aagatccagt tgctggaaaa cggcgagcaa tattatcccg cggtgtttaa ggcgattggc
     9481 gaggcacaag aacgcatcat tcttgaaacg tttatctggt ttgaggatga cgtcggcaaa
     9541 caactgcatg cggcactact ggcagcagcg caacgcgggg ttaaagcgga agtcttgctg
     9601 gatggctacg gttcgccgga tctcagcgat gagtttgtca atgaactgac ggcagctggc
     9661 gtagtgttcc gctactacga tccccgccct cgcctttttg gtatgcgcac caatgtgttt
     9721 cgccggatgc atcgcaaaat tgtggtaatc gacgcgcgta tcgcctttat tggcgggctg
     9781 aattactccg ccgagcatat gtccagctac ggtccagagg ctaaacagga ttacgcggta
     9841 cgccttgaag ggccgattgt cgaagatatt ctccagtttg agctggaaaa cctgcctgga
     9901 cagagcgcgg cacgacgctg gtggcgacgt catcacaaag cggaagagaa ccgccagccg
     9961 ggagaagcgc aggtattgct ggtctggcgc gataacgaag aacatcgcga tgatattgaa
    10021 cgtcactatc tgaaaatgct cactcaggcg cggcgagaag tcattatcgc caacgcctac
    10081 ttcttccccg gctatcgatt tttacacgcc ttgcgtaaag cggcacggcg cggggtgcgg
    10141 atcaaactga tcattcaggg cgaaccggat atgccgattg tcagagtcgg tgcgcgcttg
    10201 ctgtataact atctggttaa aggcggcgtt caggtgtttg agtaccgccg ccgcccgctc
    10261 cacggcaaag tggcattgat ggacgatcac tgggcgacag tagggtccag taatctcgat
    10321 ccgctcagtt tgtcactgaa tctcgaagca aatgtcatca tccacgatcg tcattttaac
    10381 cagacgctgc gcgataatct gaacggcatt attgccgcag attgtcagca ggtcgatgag
    10441 accatgctgc cgaaacgcac ctggtggaac ctaaccaaaa gcgtgctggc gttccacttt
    10501 ttacgccact tcccggcgct ggttggctgg cttccggcac acacgccacg tctggcgcag
    10561 gttgatcctc ccgcacaacc gacaatggaa acgcaggatc gggtagaaac tgaaaacacg
    10621 ggggtaaaac cctgatgagt aaatcacacc cgcgctggcg cttagcaaag aagatcctca
    10681 cctggctgtt ttttatcgcg gtgattgtgt tgctggtggt ctacgccaaa aaagtggact
    10741 gggaagaggt ctggaaggtc atccgcgact acaatcgcgt tgcgctgctt agtgcggtcg
    10801 ggctggtggt cgtcagctat ctgatttacg gctgctatga cctgctcgcc cacttttact
    10861 gcggtcacaa actggcgaag cgccaggtga tgctggtgtc gtttatctgc tacgccttca
    10921 acctgacgct cagtacctgg gtcggcggca ttggtatgcg ctatcgtttg tactcccgtc
    10981 tgggattgcc gggcagcact attacgcgga ttttctcgct cagtattacc accaactggc
    11041 tgggctatat tttactggca gggattatct ttaccgcagg cgtggtggag ttgccggacc
    11101 actggtatgt cgatcaaact acgctgcgta ttctcggcat tggcttactg atgattatcg
    11161 cggtttattt gtggttttgc gctttcgcga agcaccgcca tatgaccatc aaagggcaaa
    11221 agctggtgct gccttcgtgg aaattcgccc tcgcccagat gctgatttcc agcgttaact
    11281 ggatggtcat gggggcgatt atctggctgt tacttggtca aagcgtgaac tatttctttg
    11341 tactgggcgt gttgctggtt agcagtattg ccggcgtcat cgtgcatgtt ccggcgggga
    11401 ttggtgtgct ggaagcggtg tttatcgcgc tactggctgg ggagcataca tccaaaggca
    11461 caattatcgc cgccctactc gcttaccgtg tgctgtatta ctttatcccg ctgctgctgg
    11521 cgctgatctg ttacctgttg ctggaaagcc aggcgaagaa gctacgggcg aaaaatgaag
    11581 cggcgatgtg aagtttagtt aagttcttta gtatgtgcat ttacgactaa tgaaaaaaac
    11641 gcgtatgcct ttgccagaca agcgttatag ctatcgcggc aagtatccca aagaagccga
    11701 taaaatagta atagagtgtt aatgcacctg taaaagcgca acgaaccgga ctccacaact
    11761 cttcactatg gcatttcccc gccaattcaa ggagcttatt tcgtccatgc aagataatgc
    11821 ctgaccataa cacaatcatt aagcaactta ttacccaaat gggccgttca ctcattaatg
    11881 cggcattcac gatgattacc agggccaacc ctgtcaacgt catcataatc ataaaacggt
    11941 gagaaccagg atcaatatta aataataaac cggccagcat actgattaaa aacatcgcgc
    12001 ccgcagtgct gaacacgacg ataatggcat atacaggaac taaacttgca acgacaatcg
    12061 ccaccaaaga gagtaaaaac agatatatta cgcctgcgat ggcggcattt atcttgttga
    12121 tatccttaat taagccatac atacaaagta ataataaccc tatggcgaca tacattggtg
    12181 gtgaatcata tttggttgcc gtaatttcga gatacttagc cccccaggcc agccctgcgg
    12241 ttgccaccaa acctaaagcg gtaatgagcc atataccgtt taatatcctg gtctcgtgct
    12301 gaaaatctaa tttattgctg ttttgcaagt atgattccat tcttttagtt gtccttatag
    12361 aaaaatatta atattcatca tgaataaatt taaacccacg catacaaaat acttttgtat
    12421 ttatggatac ggaaaactct tccgctcggc cataaggcgt attttatacc tggtacttgt
    12481 taagtgtcgt ttttagtaag aaaaaaaatc ccgacacatg gccgggatat tgatggtgaa
    12541 aaaagaatta acgacgattg ccaaaaattc gcagcaacat caggaacagg ttgatgaagt
    12601 ccagatacaa ggttaatgcg caaagaatgg aatatttgcg caggttcgac gcgtcgcggg
    12661 tatctatctg ctcacccata tttttcagtt tctgggtgtc atacgccgtc aatccgacaa
    12721 agacaatcac gccgatgtag gtgactgccc acatcaacgc ttcgcttttc agccagaagt
    12781 tgaccagcga tgccaacaca atgccgatta acgccataaa cagcatattg ccaaagccac
    12841 ttaaatcgcg cttcgtggtg taaccgtaca ggctcattgc gccgaacatc ccggcagtaa
    12901 cgacgaaggt actggcgata gaagcagcgg tataaacaat gaaaatactg gaaagcgtaa
    12961 gacccgtcag cgccgaataa agcataaaga gcatcgtcgt tacacctgcg ctcagctttt
    13021 gaatcatcgc tgataacaca ataaccaatg ctaattgcgc gatgatcaga ccgattaaaa
    13081 agacacggtt agtgaacaac agctccatca cggccgcgga attagccgca taccaggcaa
    13141 caaatgcggt cagcaataag ccaacggtca tccagccata gacttgcgcc atataagtct
    13201 gcaagccagc ccggggttgt acaattgaat cagaacgtgg gaatctgtcc atgacgatct
    13261 cctgaagata taaggaatat cttaaggata ctgcaaaatg atgaggctgt gcatcgacgc
    13321 agcgtaaacg catgtactga gcggtgaaat tgccggacgc agcggtgcct tatccggcta
    13381 acaaaaacta ccagcgtttt gccgcctgct gatcgctctc ccgagcttcg acccagcggt
    13441 cgccctccgg cgtggcttcg cgcttccaga acggtgcgcg ggttttgaga taatccataa
    13501 taaactgccc ggcttcaaac gcactgctgc gatgcgcact ggtgacaccg ataaaaacga
    13561 tttcatcgcc cggccataat tccccgatgc ggtgaatcac cgtgacgagc cccagcggcc
    13621 agcggttacg cgcttcatcg acaatttctg ccagtgcttt ttcagtcatc cccggatagt
    13681 gttcgagggt taatgctttg acgctgtcgc ccaggttatg gttgcgcacc ttaccggtaa
    13741 aggtgactac cgcaccgtct tcgtcacgct ccgccagcca cgggtactct tctcctacgc
    13801 tgaacggctg cggaccaaca acaattttgg tttctgccat cttaacctcc ggttaccggc
    13861 gggaagaaag ctacttcgtc gccgtcagtc agcggatggt caaaactcac cagcgtctgg
    13921 ttgacggcag ccagtaattt gccatcttcc agcgccagcg cccagcgatc gctctgcgca
    13981 gccaggtgct ggcgtaacgc ttcaacggtt gggaaatccg cagccacttc ggttgcatct
    14041 gttcctacca actcgcgcac ctgggcgaaa aaaagaactt taatcatccg cttccacctt
    14101 aaagtcaccc gacttgccgc cgctcttcgc cagcaaacgt accggaccaa tcaccatatc
    14161 tttttgcacc gctttgcaca tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa
    14221 cgcttccatt tcgacaccgg ttttcccggt caggcggcat aaggtttcta tacgcacccg
    14281 attgtgctcc ggctcggcct gtaaattgac ttcaactttg ctgagcatca gcggatgaca
    14341 cagcgggatc agatcccagg tgcgttttgc cgcctgaata ccggcaatac gcgcagtggc
    14401 aaatacgtcg cctttgtggt ggcgaccatc aataatcatc gccagcgtct cgctgcgcat
    14461 ggtgacaaag gcttccgccc gcgcttcacg cacggtttcc gctttggcgg agacatccac
    14521 catgtgcgct tcgccagcgg cgttgatatg ggtcagttgc gacatactta tttcttcaaa
    14581 tgtggatgga aattacacgg acgcgtacgg gcatccagct gcggcgcgat gatattttcc
    14641 catgcggtac ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt tttgttagcg
    14701 acgcccgcta ccgcacgaga ttgcaacgtg gaagtgccaa tctcttcaaa cgacaacata
    14761 cggaacactt caccaaaacc ttcaacttca cggtcgaaca acggcagcaa tgcttcggga
    14821 gcctgatcac cttccgtcag gccagtcccc cccgtaatca acaccacttg tacatcgtcg
    14881 ctggcgatcc acgcagatac ctgagcgcga atagcgtagc ggttttcttt cacaatggct
    14941 ttatcgacaa cgtgatggcc cgcttcttgc gccgaatcgc gcaaatagtg accggaggta
    15001 tcgtcttctt caccgcgacg attagaaacc gtaagaatag caatacgggt cgggataaat
    15061 tcagtgctta cctgactcat ctgatctctc cttttgacgt tttagccgcc aatgtacgat
    15121 aagttttgcg taataccggt gttgttttga tgcaggaaat gggtctgttt cttctcccgc
    15181 agcgccgctg aaatacgcgc ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg
    15241 cgcaggttaa cgccgccttc accaaacagg cagagatgga gtttaccaat ggaggaaacg
    15301 cgcagacggt tgcaagtggc gcagaagtct ttttcatacg gcatgataag gccaatctct
    15361 ccggcgtaat ccggatgaca aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg
    15421 tggatccagc cgcgacgcag tagctcgtca cgcagaacct gaccagagat gtgatgctta
    15481 cggaaaagct cgctgccctc gcccgtttcc atcagttcga tgaaacgcag ctggataggg
    15541 cgatgctgga tccagttcag aaaggtgtcg agctggtgat gattcacatc acgcatcagc
    15601 acggtattga ctttgacctt ctcaaaaccg gcctcaaatg cagcatcgat ccctgccatc
    15661 acctgattga atttatcctg gccggtaatg gcatgaaact ggcgggcgtc cagactgtcg
    15721 acgctgacgt taatgccagt aagtcccgca tcgcgccagt tcgccacatc gcgttccaga
    15781 cggtaaccat tggtggtgac cgctatctgg cgaatagcgt cgttttctcg cacagcggcg
    15841 atgatatcgg taaagtcgcg gcgtaaagac ggctcaccgc ctgtcaggcg cactttttcg
    15901 gtgcccagac tggcgaaggc gcgcgtaacc cggcgaattt catcgacggt cagaaagcct
    15961 ttattggtga cgccgctcgg tttgtagcca tccggcaggc agtaggtgca acgaaagtta
    16021 cacacatcgg taatcgacag gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt
    16081 tgtgaagcca tgtacacctt tccagatacg ggaggcgaag tcatttcttc cttcgccctg
    16141 gtggcaatct ttacgagcat tgtcacggcc aaagcaccat atcagttgac ccaggtgcag
    16201 aggctagagt gtttagtggt taagccgata ctagcgtgta aatgagattt ttaccatcca
    16261 cactttcgct atgtaatcat gtatatagcg tcctgatcgt gctatttcgc tacagaataa
    16321 gggaaaatgg cggttttact ttgatataca tcatcaggga ggcatagcgt gcatcgtcct
    16381 ttagaggtag aagaaaatag tttgtatcct caatattggc aggttaattg ctgtttcccc
    16441 gcaatttgcg ctactgtagc gcggataatc tgactccagg aaactatatg cgcaatcgta
    16501 cgctggctga tcttgatcgt gtcgttgctc tcggcggagg gcatggactg ggacgcgttc
    16561 tctcatcact ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc acggataatg
    16621 gtggctcgac ggggcgtatt cgccgttcag aaggcggcat tgcctggggc gatatgcgca
    16681 actgccttaa ccagctgata actgaaccca gcgtcgcttc agcaatgttt gaatatcgtt
    16741 tcggcggtaa tggcgaactt tccgggcaca accttggaaa cctgatgttg aaggcactgg
    16801 atcaccttag cgtgcgacct cttgaagcga tcaatctgat tcgaaatctg ctgaaagtgg
    16861 acgcacattt gattccgatg tcggagcatc ctgttgatct gatggcgatt gacgatcagg
    16921 ggcatgaagt ttacggcgag gtcaatatcg accagttaac tacgccgatt caagagttat
    16981 tgttaacgcc taatgtaccc gcaacgcgtg aggcggttca cgctatcaat gaagcggatc
    17041 tcatcattat tgggcctggc agtttttaca ccagcctgat gccaattctg ctgctgaagg
    17101 aaatcgcgca ggcattacgc cgcacgccag cgccgatggt ctatatcggc aatctggggc
    17161 gtgagttgag tttacctgcg gccaatttga agctggaaag caagctggca attatggagc
    17221 agtatgttgg taaaaaggtc attgatgcgg tcatcgtcgg gccaaaagtg gatgtctcgg
    17281 cggtgaaaga gcggattgtg attcaggagg tgctggaggc cagcgatatt ccgtatcgtc
    17341 atgaccgcca gttgttacat agcgcactgg aaaaggcgtt acaggcttta ggttaacgga
    17401 aatccgaagg aaaattccgg cttcctaatg aagacaaagt gcgcgttgtt tatgacggat
    17461 gcggcgtgaa cgccttatcc ggcctacaaa ccgcgctaat tcaatgtatt gcagaatcat
    17521 gtaggcctga taagcgtagc acatcaggca attttgcttt tgtcatctgt cttcttcgtt
    17581 atcctgtcac gaagccgcga taaacaattc acgcagctga tgcaactggt cacgaatttg
    17641 tgccgcttct tcgaactcca gattttgcgc gtgttgcatc attaaccctt ccagctcatg
    17701 gattttctgt tgcaacgctt taggcgacat atccatcggc acattatccg gctcaacaat
    17761 cggtcgcgat tttcctctgc ccttcgcttt ggttttagca atgttctgcc ccaacgccag
    17821 gatatcgacc actttcttgt tcaagccttg cggcgtaatg ccgtgttcct cgttgtactt
    17881 ctgctgtttc tcgcggcgac gttcggtttc gccaatcgct ttcgccattg acggggtgat
    17941 cttatcgccg tagagaatcg ctttaccgtt aacgttacgc gccgcacgac caatagtctg
    18001 gatcaacgaa cgttcagaac gcaggaagcc ttctttgtcg gcgtcgagga tcgccaccag
    18061 cgaaacctcc ggcatatcca gaccttcgcg cagtaagttg atccccacca gcacgtcgaa
    18121 ctcgcccaga cgcaagtcgc ggataatctc catacgttcg acggtgtcga tatctgagtg
    18181 aagataacgc acgcgctcac cgtgttcttc gagatattcg gtaagatctt ccgccattcg
    18241 cttggtcagt gtggtgacca gtacgcgttc gttaattgcc gctcgctgac gaatctccga
    18301 aagaagatca tcaacctgtg tcgccactgg ccgcacttcg ataatcggat caagcaatcc
    18361 ggttggacgc accacctgat ccaccacatc gccgccggat ttttccagct cataattacc
    18421 tggcgtcgcc gaaacataga tggtttgcgg cgctaatgct tcgaactctt caaatttcag
    18481 cggacggtta tccagcgctg atggcaggcg gaagccgtac tccaccagtg tctctttacg
    18541 cgcccggtca ccgcgataca tgccgccaat ttgtggaatg gtgacgtgag attcatcgac
    18601 caccagcagc ccatcggcag gcaggtaatc aaacagcgtc ggcggtggct cacccggtcc
    18661 acgaccggag aggaagcgcg agtagttttc aatccccgaa cagtagccca gctcgttcat
    18721 catctccaga tcaaactggg tacgctgggt cagccgctgc tcttccagca gtttgttgtt
    18781 ttccaatagc actttgcgtc tggcggccag ctcttctttg atctcctcca tcgcctgtac
    18841 gatgcgctcg cgcggtgtga cgtagtgcgt tttcgggtag atggtaaaac gtggaatggt
    18901 ggaaacaatc tgcccggtta gcgggtcaaa taacgacaat cgttccactt cctcgtcaaa
    18961 cagttccacg cgaagtgcaa tgtcatccga ttctgccggg aagatatcaa tcacctcgcc
    19021 acgaacacgg aaagtaccac gctggaatgc ttgatcatta cgagcgtatt gcagctccgc
    19081 cagtcggcgc agaatcgcgc gctgatcgat aatcataccg accgtgagat ggagcatcat
    19141 cttgagatat aaatcaggat cgcccagacc ataaatcgcg gaaacagacg cgaccacaac
    19201 aacatcacgc cgctccagca tcgctttggt ggcggacaaa cgcatctgtt cgatatgttc
    19261 gtttaccgag gcatctttct caatgaaggt gtcggaactc ggtacatagg cttctggctg
    19321 atagtagtcg tagtaggaga cgaaatattc caccgcgttt tccgggaaga actctttcat
    19381 ttcgccatac agctgggccg ccagcgtttt gttgggcgca agtaccatgg tagggcgctg
    19441 aaggtcagca atgacattgg caatggtgaa ggttttcccg gaaccagtca caccaagtaa
    19501 cgtctggtgc gccaggccat cttccagccc ctcttcgaga cgtcgaatcg cctctggctg
    19561 atcgccagaa ggtttaaaag cggaattcag tttgaacggt ttactcatga gtcgctacct
    19621 gaaggagttg ggcgggcagg tatgtaattt tactcgtcgt acttaattat gccaacaaat
    19681 tatactggat aaaaaaacag ttcatcacca taatatttct gttacagcgt aaactccgct
    19741 gtcaatcatg agtaaaattt actctgtggc gagataaaac tcggacctta ccgggttatc
    19801 cccaaagcaa cggctttttt aacatttgtc aagatgagtg atgtcagttt tatggcaaga
    19861 gatgcctgtt cagtgactgc cattgcaatt atctaaccag ttaaaaaata aaagatattt
    19921 ctttgagccg tctttaacgc caattcgcgt gcaagccgcg tattctctcg cttgcctcgt
    19981 gttttctaac tctaatacac atggttatcc acaggaatag tggataactg tctgcagccc
    20041 ctatcccccg ccgcctgggc aattcccaca ttcgccgcat gatgcggaag taaaattttt
    20101 ttcgtgatta ttttagaatt taattggtta aattgcagtc aatcgaagac gcgatctcgc
    20161 tcgcaattta accaaataca ggatggctac aacaaggcaa ggtttatgta ctttccggtt
    20221 gccgcatttt ctggattttc tgcaagccag gggctctctc ccagcagcgg cgcgggaatc
    20281 atgcgggtga gcgtggtcat atattcagcg tgacgttttc ccggaggcgt gacatcgttc
    20341 gccacccaac ccgccagagt cagcccggcg tgttgtatcg cctgtgccgt caacatcgcg
    20401 tgattaatac agccaagttt cacgccaacc accagtatta ccggcagttg ttcctgtgtt
    20461 acccaatccg caaaagtgaa agtgtcagaa agcggcgtaa accagccgcc agcgccttcc
    20521 actaacaccc agtcagcctg ttgttcaagc gcgcgtaatc cggagctcat taccgatgat
    20581 tctatcgacc tgccctcttg cgcgctgatg atgtgcggtg aggtgggttc tgcgaaggtg
    20641 taaggattta ccgttgcgta atccagctgc aggctgctgt tgcgctgtaa cgccagcgcg
    20701 tcactattac gtaaaccctc cggggtcttt tcgctgccag aggcgaccgg tttataacct
    20761 gccgtccgat agcctgctgc ctttgcggct tgtaaaagtg cacaactggc gacagttttc
    20821 cccacttcgg tatccgttcc ggtgacaaaa taacgtttac tcacgaacaa tcactcccaa
    20881 aaaaagatga tacgtcagag gatatcgccc ctgttgttgc ggccaggcca gttgcaatcg
    20941 ctgcaactgt gaacgcgtta atattcgctg gtcgcgccct tcatgaagat gcgtggcacc
    21001 gatgcctttc agcgaacgca tggcactgag cgcatcatca aaccatagtg tgatgggctg
    21061 aatacgatgt tgataatgca cgccgttcag cgactgttcg atttcatctg gcggtaaaaa
    21121 gcgattagca tgcgggcgct cgtccaccgc ctgccacgcc tgatgcagtt cgggtaacga
    21181 tccctgcacc agcgtggtaa acgcgaccac gcctccgggg cgcaccaccc gatacagctc
    21241 gcggagtgcc gttgataaat taccgcacca ctgcaccgcg agattgctcc atgcaagatc
    21301 gaacgtcgca gtcgctaacg gcagggattc gatatctccc gccagataat ggtctgcagc
    21361 atctttctgg cgggcttgcg caagcattgg cggcgagaga tctaacgccg tcacctgcgc
    21421 gtgacgctct cgccagtggc ggctcatcca gccaggccca caccccgcgt ccagtacgtg
    21481 ggggtattta cgctgtggga gcattgccag taagacatca gcactctggc gctgtaactc
    21541 tgcgtgttgc tcatagtgcg cggctgcccg accaaatgcc gctgcaatgg cttgtttatt
    21601 aaccgttgcc atgcagcacc tccaacagac ggtcgatatc ctgcatttca tgcgcggcgg
    21661 ttagcgttaa gcgcagtcgc gcagtaccag agggtacggt tggcgggcga atcgccgtga
    21721 cccagcagcc ttgctggcgc agtttttctg ccagttgtaa cgcacggctg ttatcaccga
    21781 cgatcaatgg ctggatggcg ctgcatgaat cagcaagcgt aaacggcaaa tcctgtactc
    21841 cggcacgaaa acgcgtaatg agtgccgcca gtttttcgcg ccgtgcatca ccctcatcac
    21901 tgcgaatgac cgccagtgac gcacgtaatg cctgcgcctg agcgggcggc atactggtgc
    21961 tgtagataag atggcgggcg aattgcagca gataatccgc caccgtactg gagcaaagca
    22021 ccgcagcccc gctgacgcca aatcctttgc caaaagtcac taccagcaat tctggtttta
    22081 ccttttgcaa ccagcaggtg ccgcgcccct gctccccgat aacgcccgtg ccgtgggcat
    22141 catcgaccat caaccagcca ttgtgctgtt gcgttacctg ctggatttcc gccagtggcg
    22201 cactatcgcc gtccatgctg aacacgcctt ctgtcaccac cagttgctgc cccggacagg
    22261 gggaagcaag cagtcgcgcc agatgagtga catcgttatg aacaaaacgg cgaagctgcg
    22321 acgggcttaa actggcagcc tccagcaatg aggcatggct aagccggtca gcgacaatgc
    22381 ggtcctcttt tgccatcatc gcggcaatga ccgcctgatt agcggcaaaa ccagagataa
    22441 atagcagtgc ccgcgaatag cccagccact cggccagttc ttcttccagc gcctgatgcg
    22501 caacgctata accgctgacg tgaccggaac cgccgctacc gacgccaaat tgctccgcac
    22561 cctgcttcca ggcacggata atttgcggat gatggcttaa acctaaataa tcgttactgg
    22621 aaaagttcag atactggcga tcgtccgcca ccagccagcg tccggctcct tgcgccactg
    22681 gataacggcg acgcagggca tcggcagcac gccgcgcatc gagcgccgcg ttgattttct
    22741 cctgccagct catagtgctg ccgcgttgta atattcgtca gtgtccgggg tcatcagtgc
    22801 ctgctccaga cgctgctgtt gttcgttatc ccctgccagc acggcagttt gctgcggatt
    22861 tagccccagt ttgcgaaaca gttgcaggtc tttatcttct tccggattgg gcgtggttag
    22921 cagtttgcaa ccgtagaaaa tcgagtttgc gcctgccata aagcacatcg cctgagtctg
    22981 ttcgttcatc tgctcgcgtc cggcagaaag gcgcacgtaa gaggtcggca tcatgatccg
    23041 cgctacggca atggtgcgaa taaaatcaaa ggcatcgaca tcatcgttat ctgccagtgg
    23101 agtacctttc accttcacca gcatgttgat tggcacgctt tccggcggcg tcggcaggtt
    23161 tgccagttgc agtaataatc cggcgcgatc ttttaccgtt tcgcctaaac ccacaatgcc
    23221 acctgagcag actttgatcc cggcatcgcg cactttttcc agcgtatcaa ggcgttcctg
    23281 ataagtacgt gtggtgatga tattgccgta aaactccggc gaggtgtcga ggttatggtt
    23341 gtagtaatcc agcccggcat tcgcaaggcg ctgcgcctga gattcactca acgtacccag
    23401 cgtcatacac gcctccagcc ccatcgcttt tactccctgc accatttgtt ccaggtatga
    23461 catatcgcgt tcgtggggat tcttccacgc cgcgcccata cagaagcgcg tcgatcctgc
    23521 cgctttcgct ttgcgcgccg actccagcac ctgttcaact tccatcaacc gctcggcttc
    23581 cagcccggtt ttgtagcgcg agctttgcgg gcagtatttg caatcttccg gacaagctcc
    23641 ggtcttaatc gacagcaagg tgctgacctg cacctgacga ggatcgaaat gctggcgatg
    23701 cacctgctgc gcttcaaaca gcagatccag caacggtttt tcaaataatt ctgtgacttg
    23761 cgacaatgtc cagcgtgggc ggtgagccat ggggcttctc caaaacgtgt tttttgttgt
    23821 taattcggtg tagacttgta aacctaaatc ttttcaattt ggtttacaag tcgattatga
    23881 caacggacga tcttgccttt gaccaacgcc atatctggca cccatacaca tccatgacct
    23941 ccccgctacc ggtttatccg gtggcgagcg ccgaaggttg cgagctaatt ttgtctgacg
    24001 gcagacgcct ggttgacggt atgtcgtcct ggtgggcggc gatccacggt tacaatcacc
    24061 cgcagcttaa tgcggcgatg aagtcgcaaa ttgatgccat gtcgcatgtg atgtttggcg
    24121 gtatcaccca tgcgccagcc attgagctgt gccgcaaact ggtggcaatg acgccgcaac
    24181 cgctggaatg cgtttttctc gcggactccg gttccgtagc ggtggaagtg gcgatgaaaa
    24241 tggcgttgca gtactggcaa gccaaaggcg aagcgcgcca gcattttctg accttccgca
    24301 atggttatca tggcgatacc tttggcgcga tgtcggtgtg cgatccggac aactcaatgc
    24361 acagcctgtg gaaaggctat ctgccagaaa acttgtttgc tcccgccccg caaagccgca
    24421 tggatggtga atgggatgag cgcgatatgg tgggctttgc ccgcctgatg gcggcgcatc
    24481 gtcatgaaat cgcagcggtg atcattgaac cgattgtcca gggcgcaggc gggatgcgca
    24541 tgtaccatcc ggaatggtta aaacgaatcc gcaaaatgtg cgatcgcgaa ggtatcttgc
    24601 tgattgccga cgagatcgcc accggatttg gtcgtaccgg caaactgttt gcctgtgaac
    24661 atgcagaaat cgcgccggac attttgtgcc tcggtaaagc cttaaccggc ggcacaatga
    24721 ccctttccgc cacactcacc acgcgcgagg ttgcagaaac catcagtaac ggcgaagccg
    24781 gctgctttat gcacgggcca acttttatgg gcaatccgct ggcctgtgcg gctgcaaacg
    24841 ccagcctggc gattctcgaa tctggcgact ggcagcaaca ggtggcggct attgaagtac
    24901 agctgcgcga gcaacttgcc cccgcccgtg atgccgaaat ggttgccgat gtgcgcgtgc
    24961 tgggggccat tggcgtagtc gaaacaactc gtccggtgaa tatggcggca ttgcaaaaat
    25021 tctttgtcga acagggtgtc tggatccggc cttttggcaa actgatttac ctgatgccgc
    25081 cctatattat tctcccgcaa cagttgcagc gtctgaccgc agcggttaac cgcgcggtac
    25141 aggatgaaac atttttttgc caataacggg aagtcagcgt gagagtttct ggctacactt
    25201 tctgcaaact agaaaggagg gtttatgaaa ctcatcagta acgatctgcg cgatggcgat
    25261 aagttgccgc atcgtcatgt ctttaacggc atgggttacg atggcgataa tatttcaccg
    25321 catctggcgt gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac ctgctacgac
    25381 ccggatgcgc caaccggctc cggctggtgg cactgggtag ttgttaattt acccgctgat
    25441 acccgcgtat taccgcaagg tgatttttgt cttcttgcgc taattttttg tcaaacacat
    25501 tacgtcgttt gctgaattta caaccatgct cagtatctct ataagcgcag agaaatgatg
    25561 cagtggtggg tgggctggct tgatgaaaag gtggggtact ccacctgaac cactatcgga
    25621 atagcacaaa gccttgcaat ccagtgcaaa gttttgtgtg cctccgtttg ccccaccaat
    25681 atatattcag cacaagaaac acagctaaag caatggatgc agtcttacct gttggaatcc
    25741 cagactcact gtattgtcat catgaataag agctgagtcg tgcgggtagg aggttctacg
    25801 gggcatccca atctcttcgt ggaatttatt ttctgaaagc acctcctctg aaaaagcccc
    25861 caacccaaca gtcctggctt cttcgagtaa aagtggagag tatttttgtg attctgctcg
    25921 ggaactctca actttcaaac gctccccatt taaattgtgg aaaacatgga gcaactcatg
    25981 aaaaacaata gtcgcatgaa agtccaccac gctaatcccc tccccacagg gatattcaac
    26041 tgcattcaga ttacagtgaa aatcggaacc agtcccccga tggttctcag catctatatc
    26101 tctatgtgcc ataactccta gtctggaaga gttgaggtgt attaccactg tttctgattt
    26161 aagtcgggat attgattcaa tagcattcag gagtacacgc cctgtctctg tagagccaat
    26221 cttatcgagt gctgcttcaa tctgctgata tatattatga tcagtcccct ggaaacgaag
    26281 ttgcggatta ttttttatcc attgaacgct cgatgctttt acaagagact cgatatctgt
    26341 atctgacatt gagctcgacc ttgatgactg atgtaatacc aagttgaggg acgtagggcg
    26401 catcaggact cctgagaatg ctacttatta gcagcctgtt taccactctg tgaaaatgat
    26461 ttttttaaaa gtgaccgtca aggcggtcac caaacccgat aataaagcgg atcatagcca
    26521 tttgccagtc ctcagcgaca ttgcccattt ccgtgatccg ccaagaattg ccaaccacac
    26581 aatttgttac caactccatc catgcaacaa atccactaca ctggataaaa ttactaaatt
    26641 ttacttaata ccacactaat aagatcttgc tttccgccat gataagattg cattatttga
    26701 ctttcactcc atgaacggtc ggaaacatta taagatgata tatctattgg attaaactca
    26761 ttcttcgcgc ggtcatataa gacatttgtc tctgttgtat cgacaaaaag aattcctttt
    26821 tgctccagtc tatcaaacat atcgtaaata gcatgctcag cctgtgctgg caaggacgaa
    26881 atatttaaaa gcgattctcc atttatttta tccattctaa taccaataat atcaccatta
    26941 ttgccatata ttttttctgc actcccggca ccataatatt ggttgaagca acgaacttcg
    27001 cttgtcacct cttcattgct ttgagatgta gtaaacatct tcaggacttt tgttgcatcc
    27061 tcagcatctt catatactac agcgttcccc cctttgccaa taacattacc aggtactgac
    27121 aacctgttat agtccacact cggtaactct ggcggtgcat aatcaacagg aggtaaatca
    27181 ggtctgttcg aatgaacaat gcctctttcc atagcagcgc tcactggtga tgcattcagc
    27241 atgacctcaa ttttcctgct aatctccccc ttaggccatc ccagcctgtg caacagatta
    27301 gtaaaacaac cactatgact ttctcttgta acgcaaaact tattatcggt ggcaacaaca
    27361 cgatatgttc tgttgccaac ctttacttgc gccccattat cagaatgaac ggcagcatcc
    27421 cttacagagg ataaaacacg attatcaggc gaagtcaggt ttctggttaa agaattccat
    27481 gaacatccca aatttacaga atatggcgat aacatacatt tcaaccttca aaataaaccc
    27541 atctaaattt ccttaacaaa catcccccct aacatggcaa caaaaattag aaatgaactc
    27601 ttgctttttg tatctggcaa ctaattcatc cttccagcat cccatcaaac gaacgtttac
    27661 gaactataac gcaggtatta ccggtcactc gatatctgga gatacagacg tatcaacatg
    27721 attgagcggc atacgatact gcttccatgc cgccagtaac gatgtctgct catccgtcgc
    27781 aatatctaaa tctgcagcat cctgaagtgg tgcgatatac tcactgatta caagtaccag
    27841 accgatagat taaaaaaaac acaataacac gctgcattta aaacaaaaaa ttttaaatat
    27901 gatttaatac aagctttaag cctttaatta tcagttttat ttattttcca gcaattgatt
    27961 aatcaattgt ttatatacaa attattcctt ttctttctct cacgcaaaat tcacaaaata
    28021 ttgttgctgc aatggacctg tacaatagtg aatcacggta cttttctgtg cacaacgtac
    28081 tgcagtttct ttacgattta tagctgtact gttgaattat gtacaatttg aatccatgca
    28141 tgacgtgtga tgcctattgt gcatttttcc gagcctcttt ttactgggcc gaagccgatg
    28201 atgctgatag tctcgttccg gccatgttca cttactacag taaaattagg ggaattgcct
    28261 ttacatacaa tgttgcacga ttacccatca tcaactccgt taaaatgtat gaatagtaac
    28321 cttatgtcac tgcaaagacg aatcattgct gattgtgttt gtccacatcc ccaaatgtaa
    28381 aaatataagg ggcgccatga tacagttgga tctgatttgc atcctgtaaa ttatcgttga
    28441 tagccaatcc aggaaaatca tgttgctgga taaatccgta tcctatgtta gacacagcgg
    28501 aatactctgt gaaatcagga ctcgcctcat atcggtcact gatcgtaccc agcctttcga
    28561 agaaagccct ctcattctct tcatgcctca tggcagcctg tcgaagggca ttcaggttac
    28621 gtagcctaag atgagcttca cgttctggct ctgcatatcc tttgaagtcg ggaacactcc
    28681 atcccagttc ttgagcgaca cgacgtgcga gaatctcggt gggtcctaac tctatattac
    28741 tatctccaga tggatcgcta gatccagtaa catgatgaat aatctcgtga atcagtcctt
    28801 cctgccacga tggcatctca taagagtcag tatctggcgc aacactaaaa ctgacataag
    28861 gctcttcgtt atcgttttct cctgcttcgc aaatggggaa aattggctct tgcccgacat
    28921 catattcata taattcgtta caggttagtg attgaatatc atcaattttg acagaagatt
    28981 cttcgttaag ctcgtattcg tttctgtatt taatgcaacc aatgtgtacc ttctcattat
    29041 gaatgccata gcttacggca tcacgaaatg tctgtgatcg gctcaaagca tcaagtacag
    29101 tgttgccgat catatcgact gtatgctgat cgataagcct gctacggctg tcatggacgg
    29161 cgcgtattac actttcgtat acgttgctta aatctgctgc ggaaagtggt attcgcttac
    29221 ctatatccaa aacgtaatcc gcataggcat tttcagcacg attaggagca atgggagcag
    29281 agtatcctgc cggggcggaa aagttgaagt tggactgtaa tgagggaatt ttcatgttta
    29341 tatctaatac cctgcaaatc atttattaca acaaaataaa ctatatcaaa ttcatttacc
    29401 atgaactgca tgta
//
LOCUS       DAZKIF010000054        29149 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZKIF010000054 DAZKIF010000000
VERSION     DAZKIF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.054"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            299..1165
                     /gene="folD"
                     /locus_tag="ACHOTW_002331"
     CDS             299..1165
                     /gene="folD"
                     /locus_tag="ACHOTW_002331"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP9844433.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1167..1379
                     /gene="ybcJ"
                     /locus_tag="ACHOTW_002332"
     CDS             1167..1379
                     /gene="ybcJ"
                     /locus_tag="ACHOTW_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP9844434.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1487..2008
                     /gene="ybcI"
                     /locus_tag="ACHOTW_002333"
     CDS             1487..2008
                     /gene="ybcI"
                     /locus_tag="ACHOTW_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9844435.1"
                     /translation="MPTVITHAAVPLCIDLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2044..3429)
                     /gene="cysS"
                     /locus_tag="ACHOTW_002334"
     CDS             complement(2044..3429)
                     /gene="cysS"
                     /locus_tag="ACHOTW_002334"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9844436.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3603..4097
                     /gene="ppiB"
                     /locus_tag="ACHOTW_002335"
     CDS             3603..4097
                     /gene="ppiB"
                     /locus_tag="ACHOTW_002335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP9844437.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4100..4822
                     /gene="lpxH"
                     /locus_tag="ACHOTW_002336"
     CDS             4100..4822
                     /gene="lpxH"
                     /locus_tag="ACHOTW_002336"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP9844438.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4940..5449
                     /gene="purE"
                     /locus_tag="ACHOTW_002337"
     CDS             4940..5449
                     /gene="purE"
                     /locus_tag="ACHOTW_002337"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9844439.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5446..6513
                     /gene="purK"
                     /locus_tag="ACHOTW_002338"
     CDS             5446..6513
                     /gene="purK"
                     /locus_tag="ACHOTW_002338"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9844440.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(6652..7545)
                     /gene="ybcF"
                     /locus_tag="ACHOTW_002339"
     CDS             complement(6652..7545)
                     /gene="ybcF"
                     /locus_tag="ACHOTW_002339"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9844441.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7542..8357)
                     /gene="ylbF"
                     /locus_tag="ACHOTW_002340"
     CDS             complement(7542..8357)
                     /gene="ylbF"
                     /locus_tag="ACHOTW_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9844442.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8368..9627)
                     /gene="ylbE"
                     /locus_tag="ACHOTW_002341"
     CDS             complement(8368..9627)
                     /gene="ylbE"
                     /locus_tag="ACHOTW_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9844443.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9637..11304)
                     /gene="fdrA"
                     /locus_tag="ACHOTW_002342"
     CDS             complement(9637..11304)
                     /gene="fdrA"
                     /locus_tag="ACHOTW_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9844444.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11621..12670
                     /gene="allD"
                     /locus_tag="ACHOTW_002343"
     CDS             11621..12670
                     /gene="allD"
                     /locus_tag="ACHOTW_002343"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP9844445.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12692..13927
                     /gene="allC"
                     /locus_tag="ACHOTW_002344"
     CDS             12692..13927
                     /gene="allC"
                     /locus_tag="ACHOTW_002344"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP9844446.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13938..14723
                     /gene="allE"
                     /locus_tag="ACHOTW_002345"
     CDS             13938..14723
                     /gene="allE"
                     /locus_tag="ACHOTW_002345"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP9844447.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14951..16096)
                     /gene="glxK"
                     /locus_tag="ACHOTW_002346"
     CDS             complement(14951..16096)
                     /gene="glxK"
                     /locus_tag="ACHOTW_002346"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP9844448.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16118..17419)
                     /gene="ybbY"
                     /locus_tag="ACHOTW_002347"
     CDS             complement(16118..17419)
                     /gene="ybbY"
                     /locus_tag="ACHOTW_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFP9844449.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17476..18837)
                     /gene="allB"
                     /locus_tag="ACHOTW_002348"
                     /pseudo
     CDS             complement(17476..18837)
                     /gene="allB"
                     /locus_tag="ACHOTW_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(18874..20265)
                     /gene="ybbW"
                     /locus_tag="ACHOTW_002349"
     CDS             complement(18874..20265)
                     /gene="ybbW"
                     /locus_tag="ACHOTW_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP9844450.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(20434..21312)
                     /gene="glxR"
                     /locus_tag="ACHOTW_002350"
     CDS             complement(20434..21312)
                     /gene="glxR"
                     /locus_tag="ACHOTW_002350"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9844451.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21413..22189)
                     /gene="hyi"
                     /locus_tag="ACHOTW_002351"
     CDS             complement(21413..22189)
                     /gene="hyi"
                     /locus_tag="ACHOTW_002351"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFP9844452.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22202..23983)
                     /gene="gcl"
                     /locus_tag="ACHOTW_002352"
     CDS             complement(22202..23983)
                     /gene="gcl"
                     /locus_tag="ACHOTW_002352"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP9844453.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24073..24888)
                     /gene="allR"
                     /locus_tag="ACHOTW_002353"
     CDS             complement(24073..24888)
                     /gene="allR"
                     /locus_tag="ACHOTW_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP9844454.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24966..25448)
                     /gene="allA"
                     /locus_tag="ACHOTW_002354"
     CDS             complement(24966..25448)
                     /gene="allA"
                     /locus_tag="ACHOTW_002354"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP9844455.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25678..26604
                     /gene="allS"
                     /locus_tag="ACHOTW_002355"
     CDS             25678..26604
                     /gene="allS"
                     /locus_tag="ACHOTW_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP9844456.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26673..27767
                     /gene="mnmH"
                     /locus_tag="ACHOTW_002356"
     CDS             26673..27767
                     /gene="mnmH"
                     /locus_tag="ACHOTW_002356"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP9844457.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            27883..28248
                     /gene="ylbG"
                     /locus_tag="ACHOTW_002357"
                     /pseudo
     CDS             27883..28248
                     /gene="ylbG"
                     /locus_tag="ACHOTW_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28998..>29149)
                     /locus_tag="ACHOTW_002358"
     CDS             complement(28998..>29149)
                     /locus_tag="ACHOTW_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844458.1"
                     /translation="LVSKHYINYMAIPENDGVFTWLPDFFPHVAVDISIYTNVEDDYF
                     FLIFP"
BASE COUNT         7216 a         7876 c         7213 g         6844 t
ORIGIN      
        1 atatattcaa cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta
       61 tattgtcagg tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag
      121 atttacaacg ccatcatgga cgaaaaatga agctttcaat ctcagcgaca gtgcgcctaa
      181 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
      241 ttctgacaaa ataggcgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
      301 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
      361 aaaagttcag gcgcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
      421 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgtaaggctt gtgaagaagt
      481 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
      541 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
      601 accggcgggt attgataacg tcaaagtgct ggaacgtata cacccggaca aagacgtaga
      661 cggtttccat ccttataacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
      721 caccccgcgc ggtattgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
      781 cgccgtggtg attggcgcat cgaatatcgt tggccgcccg atgagcatgg aactgttgct
      841 cgcaggttgc accaccaccg tgactcaccg tttcactaaa aatctgcgtc accacgtaga
      901 aaatgccgat ctgttgatcg ttgccgtcgg caagccgggc tttattcctg gtgactggat
      961 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     1021 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttccggg
     1081 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     1141 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     1201 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     1261 tcgcgattgc cgaaggccag gtgaaagtcg acggcgcggt tgaaacgcgc aaacgctgca
     1321 aaatcgtcgc cgggcagaca gtgagttttg caggtcacag cgtacaggtt gttgcctgat
     1381 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     1441 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     1501 tacgcacgcc gccgttcccc tatgcattga tttagggttg ggttcgaaag tcatccctcc
     1561 gcgcctgtta tttgccggaa tcatcctggc gatgctgccc gatgccgacg tcttgtcgtt
     1621 taattttggc gttgcttacg gcaatgtttt tggtcatcgc gggtttaccc attcgctggt
     1681 gtttgcgttt gttgtcccgc tgttatgtgt gctgattgga cgtcgatggt tcagggctgg
     1741 gctgattcgc tgctggctgt ttttaaccgt ctcgttgcta tcgcacagct tgctggattc
     1801 ggtaaccact ggcggtaaag gggttggctg gctgtggccg tggtcagatg aacgcttttt
     1861 cgctccctgg caggtgatta aagtcgcgcc gtttgcattg tctcgttaca ccacagcgta
     1921 cgggcatcag gtgattattt ctgaattgat gtgggtgtgg ttgccgggga tgctactgat
     1981 gggaatgttg tggtggcgca gacgataacc ggatgcgaaa actcgcatcc ggcaatagcg
     2041 caattactta cgacgccagg tggtcccttg cgggccatct tccagcacga tccccatctc
     2101 attaagacgg tcacgcgccg catctgccgc cgcccagtct ttcgctttac gggcatccag
     2161 acgctgttga attaacgctt caatctcagc cacttcgctg tcgtctgcct gcgcgccgct
     2221 ttgcaggaac gcttccggtt cttgctccag caggcccaat acggcggaaa gtttacgcag
     2281 gtgagatgcc attgcattcg ccgctgccat atcttctact ttcagacggt ttacttcacg
     2341 cgccatatca aacagcacgg aataggcttc cggggtgttg aaatcgtcgt ccatcgcttc
     2401 aataaagcgc gcttcaaacg cttcgccacc ggcaggcgca acggttttat ctgtgccgcg
     2461 cagcgcagtg tagagacgct ccagcgccgc acgcgcctgc ttcaggttct cttcgctata
     2521 gttcagctgg ctgcgatagt ggcccgacat caggaagtaa cgcacggttt ccgcgtcgta
     2581 gtatttcagc acatcgcgca cggtaaagaa gttacccagc gatttggaca tcttctcgcg
     2641 gtcaaccatc accatccccg agtgcatcca gtagttcaca tactgaccat catgggcaca
     2701 ggtggactgc gcgatttcgt tttcgtggtg cgggaacatc aggtctgaac cgccgccgtg
     2761 gatatcaaag tggttaccca gctgcttgca gttcattgcc gaacattcaa tgtgccagcc
     2821 aggacgaccc gcgccccacg gagacggcca gctcggttcg ccctctttcg acatcttcca
     2881 cagaacgaag tccattgggt tgcgtttgtc gtcgaccacg tcaacgcgcg cgcctgcctg
     2941 cagctggtcg agatcctgac gcgacagcac gccataagtt ggatcggtcg ggacgtcgaa
     3001 catcacgtcg ccgttgtccg ccacataagc gtgacctttg gcgatcagtt gttcagtgag
     3061 ttcaataatt tctgcgatat ggtgcgtcgc gcgcggctcc atatccgggc gcagaatgtt
     3121 caacgcatca aaatctttgt gcatttcggc gatcatgcga tccaccagcg ccacaaagct
     3181 ttcgccattt tcattggcgc gtttgatgat tttgtcgtcg atatcggtaa tgttgcgcac
     3241 atacttcagc ttatagccga ggaaacgcag atagcgcgca accacgtcaa aagcaacaaa
     3301 ggtacgcccg tgaccgatat gacagagatc gtaaacggtg attccacaca cgtacatgcc
     3361 gacttccccg gcgtgaatag gcttaaattc ctctttttgg cgtgtcagag tattgaagat
     3421 ttttagcatc gaagattccg tttagacatg tgtgggtaat tgagttgcgt ataataccca
     3481 tatttcccgc gcgaatcagc atacattgcg tgatgatccg atcgtgcggt tatgctataa
     3541 caccacccta tatatgaccc gaactgggtt gaagcaccaa tcaaacggaa caggatgcaa
     3601 aaatggttac tttccacacc aatcacggcg atattgtcat caaaactttt gacgataaag
     3661 cacctgaaac agttaaaaac ttcctggact actgccgcga aggtttttac aacaacacca
     3721 ttttccaccg tgttatcaac ggctttatga ttcagggcgg cggttttgaa ccgggcatga
     3781 aacaaaaagc caccaaagaa ccgatcaaaa acgaagccaa caacggcctg aaaaataccc
     3841 gtggtacgct ggcaatggcc cgtactcagg ctccgcactc tgcaactgca cagttcttca
     3901 tcaacgtggt tgataacgac ttcctgaact tctctggcga aagcctgcaa ggttggggct
     3961 actgcgtgtt tgctgaagtg gttgaaggca tggacgtggt agacaaaatc aaaggtgttg
     4021 caaccggtcg tagcggtatg caccaggacg tgccaaaaga agacgttatc attgaaagcg
     4081 tgaccgttag cgagtaatcg tggcgacact ctttattgca gatcttcatc tctgcgtgga
     4141 agaaccggcg atcaccgccg gttttctgcg ttttttagcg ggagaagccc gcaaggccga
     4201 cgcgctgtat attcttggcg atctgtttga agcatggatt ggcgacgacg atcccaaccc
     4261 actccatcgc cagatggcgg cagcgatcaa agcggtgtcc gattccggcg ttccctgtta
     4321 tttcattcat ggcaaccgtg attttctgct cggcaaacgc tttgcccgtg aaagtggcat
     4381 gacgttattg ccggaagaaa aagtgctcga actttatggt cgtcgggtgc tgattatgca
     4441 tggcgacacg ctgtgcaccg atgacgcggg ttatcaggct tttcgcacca aagtccacaa
     4501 accctggctg caaacgctat tcctcgctct gccgttgttt gtgcgcaaac gcattgccgt
     4561 gcgaatgcgc gcgaacagta aagaagccaa cagcagtaaa tcgctggcga tcatggacgt
     4621 taaccaaaat gcggtagtca gtgcgatgga aaaacatcag gtgcaatggc tgatccacgg
     4681 gcatacccat cgcccggcgg tgcatgaact tatcgccaat cagcaacctg cttttcgcgt
     4741 ggtactgggt gcctggcata cggaaggttc gatggtgaaa gtcacggcgg atgacgttga
     4801 gctgattcat tttccgtttt aaaaaacccg caactttgct gatttcacag ccacgcaacc
     4861 gttttccttg ctctctttcc gtgctattct ctgtgccctc taaagccgag agttgtgcac
     4921 cacaggagtt ttaagacgca tgtcttcccg caataatccg gcgcgtgtcg ccatcgtgat
     4981 ggggtccaaa agcgactggg ctaccatgca gttcgccgcc gaaatcttcg aaatcctgaa
     5041 tgtcccgcac cacgttgaag tggtttctgc tcaccgcacc cccgataaac tattcagctt
     5101 cgccgaaagt gccgaagaga acggttatca ggtgattatt gcgggcgcag gcggcgcagc
     5161 gcacctgcca ggcatgattg ccgccaaaac gctggtgcca gtgctgggcg tgccagtaca
     5221 gagcgccgca ctgagcggtg tcgatagcct ctactccatc gtacaaatgc cacgcggcat
     5281 tccggtgggt acgctggcga ttggtaaagc tggcgcggca aacgcggcat tactggcagc
     5341 acaaattctt gcgactcatg ataaagaact gcaccagcgt ctgaatgact ggcgcaaagc
     5401 acaaactgat gaagtgctgg aaaacccgga cccgcgaggt gcggcatgaa acaggtttgc
     5461 gtcctcggta acgggcagtt aggccgtatg ctgcgtcagg caggcgaacc gttaggcatt
     5521 gctgtctggc cggtcgggct ggacgctgaa ccggcggcgg tgccttttca acaaagcgtg
     5581 attaccgctg agatcgaacg ctggccggaa acagcattaa cccgcgagct ggcgcgccat
     5641 ccggcctttg tgaaccgcga tgtgttcccg attattgctg accgtctgac tcagaagcag
     5701 cttttcgata agctccacct gccgactgca ccgtggcagt tacttgccga acgcagcgag
     5761 tggcctgcgg tgtttgagcg tttaggtgag ctggcgattg ttaagcgtcg cactggtggc
     5821 tatgacggac gcggtcaatg gcgtttacgc gccgatgaaa ccgagcagtt accggctgaa
     5881 tgctacggcg aatgtattgt cgagcagggc ataaacttct ctggtgaagt gtcgctggtt
     5941 ggcgcgcgct gctttgatgg cagcaccgtg ttttatccgc tgacgcataa cctgcatcag
     6001 gacggtattt tgcgcaccag cgtcgctttt ccgcaggcca atgcgcagca gcaagcgcaa
     6061 gccgaagaga tgctgtcggc gattatgcag gagctgggct atgtgggcgt gatggcgatg
     6121 gagtgttttg tcaccccgca aggtctgctg atcaacgaac tggcaccgcg tgtgcataac
     6181 agcggtcact ggacacaaaa cggtgccagc atcagccagt ttgagctaca tctgcgggcg
     6241 attaccgatc tgccgttacc gcaaccagtg gtgaataatc cgtcggtgat gatcaatctg
     6301 attggtagcg atgtgaatta tgactggctg aaactgccgc tggtgcatct gcactggtac
     6361 gacaaagaag tccgtccggg ccgtaaagtg gggcatctga atttgaccga cagcgacaca
     6421 tcgcgtctgt ccgcgacgct ggaagccttg atcccgctgc tgccgccgga atatgccagc
     6481 ggcgtgatgt gggcgcagag taagttctgt taacctggtg ctcatttgat tgccggatgc
     6541 ggcgtgaacg ccttatccgg cctacaccga tccggtaccc attgtaggcc tgataagacg
     6601 cgtcaagcgt cgcatcaggc atggtgcgcc gattgccgga tgctctcatc actacagcga
     6661 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6721 cgcgggttta ccacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6781 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6841 ctgcggcatt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6901 ggcgttaatt tgctcggcga gcaacgcagc ggcgagatct ttatcaatca ctgcttcact
     6961 ccctgctccg tcatccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     7021 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     7081 caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag
     7141 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7201 cggatcatca ggcgaaactt caatgcgcgt ccgcaccgtc gtgacaggcg gcatctgcgg
     7261 ctgtgcgctc aaactctgcg ccagcatata gccaatcatc ccctgacttt ctgccaccag
     7321 cacatccagc ggatacggtt ctacctcttt ccacgccaga ttctgcaatg ccagcagccc
     7381 cacctgcggc ccgttgccgt gaacaatcgc cagccgataa gaacgggcca ggcgcgccag
     7441 cgcgggtaca gcactggcga tattgcgata ttgattttct gccgtcagcg cctcaccgcg
     7501 ctggagtaag gcgttgcccc caagagctac aaccagtgtt ttcatggttt tccttgtaat
     7561 aattgttggc caagccagaa ccccagcagt gtatctgccc ctgacgtatg ccccagcgcc
     7621 agcagcgaat caatcgcaac agcggtacgt ttcgggcaac tcagagcatg aacaaagtgc
     7681 aggagtggcg aggcgaaata tccttgcgcg gcataacgta aataactgac gctcaccgct
     7741 gtggtaacga gttgcagatt gtcggaacag gcaaaaaacg gacgaccgga gcgcgcatct
     7801 aaagcaccat aataccaggc cgccagcagc attccgctca gcgtgtcatc atgacttggc
     7861 gttaatccgg ggcctttacc cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg
     7921 gcctgaaaac agtgacgaaa ctggcgtaac tcagcaggca gcggatcgct tgccgccaac
     7981 gccagtggcc cgaaaagccc ggtttcctcc gcgcgttgca tccatgcagc tgcaagtggt
     8041 tgaggatgcg caggcggcgt aatacgcagc aaacaataac gctgtggctg tttaaccgtg
     8101 aaacgcccga ggcgaatccc ttgagccaca acctgtggtc gttcattgcc gcataatcca
     8161 ccgcataacg catcgaattg cgcacggcga agcacccatc ctccggggcc gaaaccacta
     8221 ccctgacgat gcagcgttaa cagttcgccg ctttccgtca tcaggttaat cgcccgccgc
     8281 cacactcccg ctaaccgcca ggactggcga taattcggtg cgctactact ggcaagcaga
     8341 ggatggatga tcgtcattac gcgttcctca accaatcccc atgctttcag ccagtgcttc
     8401 cagcgcctgt tcaaagcacg ccagcggtgc ccgcacggtg cctgcgccaa tctgcccgat
     8461 ccccgcctct ttatgggcga taccggtatt gatgagtggc gtaatgccgg tttctaccac
     8521 gcgacgaatg tccagtccca ggcacgcgcc ctgaaaatcc cagcttggga tctgcaactg
     8581 catattgcgt tcaaggtaaa tttccgccat ctcttcagat accgccctgg ccgcttccat
     8641 gccactcgca ccgacaaagc gcgttacgcc aggcgctgcg atcattgccg cacctccgat
     8701 accaaaggtt tcggtaatcg cgctatcgcc catatccggg ttcgcctgct cctgcgagaa
     8761 gccggtgaaa aacagacctt gcggagtgtt tacaggcgca gtaaaccagc gttcgcccag
     8821 cccgcttacc cgaatcccga acatattgcc gttgcgggtc attgccgtga cgatgctgcc
     8881 tgcgcggatc atcgcgccag catccatcgc cgccttgcag taagccatcg cgaggttgag
     8941 gaagaactga tcggtcacgc tgaggaaatc catcacttcc gcgatgtgct gtttatcatg
     9001 atcgaggcga gcaatttgtg gggccagcgc acgcattaac agtgctgagg aagcaatatt
     9061 gcgttgatgg aactcatcgc ccatcgtaat gccctgcgcc atcatcgcag taagatcgat
     9121 accgcgctcc atgcgcccca gcgccgcgct taatactggc attaacacat cgcgcatcca
     9181 gcgatggcga gtcaggacat cttcgccgta agcgccaaaa cgcatcactt tgccgatacc
     9241 ttcgttgagg ttgcagtacg cccggttgcc gtcggtcacg ttctcgacca ccagcatcgg
     9301 catactggca gaagtaatac cgcccattgg cccgacggca ttcacatggt gacaaggaat
     9361 gaagttcact tccccctgct ccagtattgc cagcgcctgc gcttcatctt tcgcccaacc
     9421 ttcgaacaga catgcgccca cgcacgcccc tttcatgggt ccggtcatct cctgccagcg
     9481 cattggcggc ccggcgtgaa gcagtgtttt gccctcgttt agttcgctga taagtgaaga
     9541 agccggttgc acatccagcc agtgtggacg agcgcgacga atttgttcga taaccgcagc
     9601 attggcttgc gccactgatg taaacatgag aaccccttat tgcaaacgtt ctaataaacg
     9661 agccagtttt ttattgccgc cggcgactgg cgaccattgg taatgcacaa ccggtttgct
     9721 ggcgctttgt agctccagcg caaaactgcg taatccgatg ttaatcacgg cgacgttttc
     9781 cagtaatgac ggtgtgtgtt gctgtgcggc aggcgagagc ggatgaatta acgctgccgc
     9841 cagcaaggtg gcttccggta gcgaactcac gaccgcaatc cccgcatctt ccagcgtggc
     9901 gatttgctgc gagcggcatt gcgggtcacg ttcagtgcct gtcaccgtgg caatggcata
     9961 cagtggttga ttgtctgaac gcgcagcaca ggctttttgc caggcgctta ccagcgaggc
    10021 ggcaggatcg gcggtcgcac cgaagccaat cacgacatca agcagcagca cacgcacttg
    10081 cggtttagcg ccgagatcgg taattaactg gttgcgtaag gttgggtcga tcatcggatg
    10141 gggacgcccg acggtgtaga aatcatcgcc gaggtcaata atctggtggc catcggcgtc
    10201 cagcatcatg ccgtgatgat gggcatcatc ggcttccacg ccaaggtgtc cggcaagtaa
    10261 tcccgccgct tcggcagcca gcgtaccgcc ggtatacaaa ccgcaaataa atccgctgct
    10321 ggcaggcatt atcgcgttac gtcgcgccgt aacgcgtgaa agcaggcaag ccaggcgtgc
    10381 ggcctcatcc agcgaggagg caaaccagac attctcgtcg cgggccaccg ccggggtata
    10441 acctaaaaac agcgccaccg tcggtttgcc ggttgctttc atggcattaa caattttcag
    10501 acgcaccgct tcggcaggtg gttttgaaac aaatgccagc acttcgcttt tctcgtctgc
    10561 actgagcatt tccagcgccg tcagcgcact gatgccgccc acttcacggc tgaggtcgcg
    10621 gccgccaagg ccaatcgcgt gagtaattcc ctcccctgcc agcgcaatct gcgagcacag
    10681 ctcctgaatc ccggtaccgg aagcgccaat gacgccaatg ttgccttccg gcatcacgtt
    10741 agcaaaagcc agcggcgtgc cggcaatcat cgacgtaccg cagtccggcc ccatcaccag
    10801 caagcctttt tcccgcgcgc gggttttaag ttggatttca tcttccagcg tgacgttatc
    10861 ggagaacatc atcacgttga ggttgcgatc cagcgcctga ttcgccagct ccgccgcata
    10921 ctcgccagcc actgaaatca gcgccagatt ggcatcgggt aatttctgac aggcactgtc
    10981 ccagcgacgc acctgcgtca acgcctggct gctgcctgac ccctgcgcca gttgttttag
    11041 cgcctcttca agctgctgca taatcgcctg cgcgatcccc gcatccgccg cttcgctacg
    11101 aattgccacg caaatatcgt tcggcgtggc gttattaaaa tcgtcatgcc agaaacctgt
    11161 ggtatctaat aacgctttat tggcgggcgt acccatcatt acggaaacat catcaacatt
    11221 ttctgattcg ctgagttttc gtgaaataat cattaaactg accgaatcct gaaaacaccc
    11281 ttttttaata aaggcgtgga tcataacaac tccttaaaaa ttgcacccat ggcaattgca
    11341 cgagcagtgt taatgtggcg attacggtat cgatgacaac gagtaaataa ggtgatgaat
    11401 atcacacagg gattattaac aaaagaattc aaataaccgc catgctatta cgtgttattt
    11461 tttaataagc gtggttgttt taaaaaccag gttaaaagcg agcggattat caaataaatt
    11521 ctaactatat ttttttgcct gtctggatca cataatccag atattttccc tgtatgttaa
    11581 ttgcagtcat gcttcacacc gtcgttaaaa aggaagacag atgaaaatca gtcgggaaac
    11641 actccaccag ctaattgaga ataaactctg ccaggctggg ttaaaacgtg agcacgctgc
    11701 aaccgtggct gaagtattgg tttatgccga tgccagaggg atccactctc atggcgcggt
    11761 gcgcgtggaa tactacgcgg aacgcatttc aaaaggcggc accaaccgcg aaccggagtt
    11821 tcgtcttgag gaaaccgggc cgtgctcggc aattttacat gccgacaatg ccgccggaca
    11881 ggtcgcggcg aaaatgggta tggaacatgc catcaaaacc gcccagcaaa atggcgttgc
    11941 ggtggtcggt atcagccgga tgggtcacag cggcgcaatc tcttattttg tgcagcaggc
    12001 agcccgcgcc ggattaattg gcatttcgat gtgccagtcc gatccgatgg tggtgccgtt
    12061 tggcggcgcg gaaatttact acggtactaa cccactggcc tttgccgcgc cgggagaagg
    12121 cgacgagatc cttacctttg atatggcgac taccgtacag gcatggggaa aagtcctcga
    12181 cgcccgctcg cgtaatatgt ctatcccgga tacctgggcg gtcgataaaa acggtgcacc
    12241 aacaaccgat ccgttcgcgg tacatgctct gctccccgca gccgggccga aagggtatgg
    12301 cctgatgatg atgattgacg tcctctcagg cgtcttactc ggcttaccgt tcgggcgaca
    12361 ggttagttcg atgtatgacg atttacacgc cgggcgtaat ttggggcaat tacatgtagt
    12421 tattaacccg aactttttct cctccagcga attattccgt caacatctta gccagaccat
    12481 gcgcgaatta aatgccatta cccccgcgcc cggttttaat caggtttatt atcccggaca
    12541 ggatcaggat attaaacaac gccaagccgc cgtcgaaggc atcgaaattg ttgatgatat
    12601 ttaccagtat ttaatttccg acgcgcttta taacacgtca tacgaaacga aaaatccctt
    12661 tgcgcaataa ttattgagac aggactttct tatgattaca cattttcgtc aaaccataga
    12721 agaagcgctg ccctggcttt cctcttttgg cgctgaccca gcgggtggga tgacccgttt
    12781 actttattcg ccggaatggc tggaaaccca gcagcaatta aaaaaaagaa tggccgcaag
    12841 tgggctggaa acacgtttcg atgaagtggg gaatttatac ggtcgcctga gtggcaccga
    12901 atatccacag gaagtgattc tgagcggttc gcatatcgat accgtggtta acggcggtaa
    12961 cctcgacggg caattcggcg cgctggcggc gcggctggca attgactggc tgaaaactca
    13021 atacggcgca ccgctgcgta cggttgaagt ggtggcgatg gcggaagaag aaggtagccg
    13081 cttcccgtat gttttctggg gcagtaaaaa tatctttggg ctggcgaatc ctgacgacgt
    13141 gcggaatatc tgtgatgcca aaggaaatag ttttgtcgat gcgatgaagg cttgcggatt
    13201 tactctgccg gacgccccgc taactccgcg tcaggatatt aaagcctttg tcgaactgca
    13261 tatcgaacag ggctgtgtgc tggaaagtaa tgggcaatca attggcgtgg tgaatgcaat
    13321 tgtcgggcaa cgtcgctata cggtgacgct gaacggcgaa tcaaaccatg caggcaccac
    13381 gccgatgggt tatcgtcgtg atacggttta cgctttcagt cgcatttgcc atcagtcgat
    13441 cgaaaaagcg aaaaagatgg gcgatccgct ggttctgacc tttgggaaag tagagccgcg
    13501 cccgaatacg gtgaatgtgg tgccgggtaa aaccacgttc accattgatt gtcgtcatac
    13561 cgacgccgcc gtgctgcgtg atttcaccca acagttagaa aacgacatgc gggcgatttg
    13621 cgatgaaatg gacattcgta ttgatatcga tttatggatg gacgaagaac ccgtgccgat
    13681 gaataaggac ctggtcgcca ccctgacaga attgtgtgaa agtgaaaaac tgaattaccg
    13741 ggtgatgcac agtggtgccg ggcacgacgc gcaaattttc gcgcctcgcg taccaacctg
    13801 catgattttt atccccagca tcaacgggat cagccataac ccggcggaac gcaccaatat
    13861 taccgacctt gccgaagggg tcaaaacgtt ggcactcatg ctttatcaac ttgcctggca
    13921 gaaataagga gtcataaatg ggatatttaa ataacgtcac cggttaccgc gatgatttac
    13981 tggctaaccg tgcgattgtg aaacacggta atttcgcact gttaacacca gacggtctgg
    14041 taaaaaatat tattccgggc tttgaaaatt gtgacgcgac aatcctctcc acgccaaagc
    14101 tgggtgcctc ttttgttgat tatctggtca cactgcatca aaacggcggc aaccaacagg
    14161 gcttcggtgg cgaaggcatt gaaacgttcc tgtatgtgat ctctggaaat atcactgcca
    14221 aagccgaagg caaaacattt tccttaagcg aaggtggcta tctttattgc ccgcctggct
    14281 ccttaatgac gtttgttaac gcccaggccg aagacagcca aatcttttta tataagcgcc
    14341 gctatgttcc ggtagaaggc tatgcaccgt ggctggtttc tggcaatgcc agcgaactgg
    14401 aacgcattca ctatgaaggt atggatgatg tgatcctgct ggatttcctg cccaaagagt
    14461 taggttttga tatgaacatg catatccttt cttttgcacc aggtgccagc cacggttata
    14521 tcgaaacaca tgttcaggaa cacggtgcct atattctttc cggtcagggg gtttataacc
    14581 tcgacaataa ctggatcccg gtgaaaaaag gcgattacat ctttatgggc gcttattctt
    14641 tacaggctgg ttatggcgta gggcgtggtg aagcgttcag ctatatttac tcgaaagatt
    14701 gtaaccgcga cgtagagatt taagtcatac cactcgcctg attattttta gctatgttgg
    14761 attttgccac ggtttatacc gtggcttttt ttgcaacata gcctgatgcg cttcgcttat
    14821 caggcctaca tgatctctgc aatatataga atttgcgcga ttttcaggaa ggatatggta
    14881 ctcatgccgc atccggcatg agtactgcac tcttatgcga tataaatcgc atccgcttta
    14941 aaggtaaggg tcagttttta attccctgac ctattttaat ggcgcaggca atatttcgcg
    15001 cgctgttgaa gagattggtt tcaccgctgg cgagcacttc ggctaaaggt gccagacgtg
    15061 gcaaaatgct gaataccgca tcaatgccgt actggtgcac cacttccacg ccatcaccca
    15121 atacgccagc aatcccaatc accggtacat taaactgctt cgccaccgac gcaacgccca
    15181 gcggcgcttt accgcctgcc gtttgcgagt caatgcgccc ttccccggtg atcaccagtg
    15241 ctgccccctg caccgcctgc tccaggttga ccgcccgcag tacaatctca atacccggtt
    15301 taatatccgc attgaggaat accgccgccg caatccccat accgcccgca gcgccaccgc
    15361 ccgccatctg gcagacatta atttcagttt gctgttgtaa aacacgggcg taattttgca
    15421 gcccctgttc aagttcttcg accatctccg gcgttgcccc tttttgcggg ccaaacaccg
    15481 ccgccgcgcc gcgtgcccct accagcgggt tatcaacgtc acatgctact tcaatatggc
    15541 agttcgccag acgcggatcg cattcctcca tctcaatgct tgccacgcgc gctaaattgc
    15601 cgccgttagc caccagcacc tgaccgtcgg catcaaggaa acgcacaccg agcgcctgcg
    15661 ccatgcccat accgccgtcg accgtcgcac tgccgccaat gccgagaata atatggcgaa
    15721 tgccgttatc cagcgcatga cgaattaact cccccgtacc aaaactggag gccagcaacg
    15781 gattacgctt ttcaggcgcg accagcatca ggccacttgc tgccgccatc tcaatcaccg
    15841 ccgttttccc gtcgccggta atgccataaa aagcattcac tttttcgccc atcggcccgc
    15901 agacttcaag cgtcacgatg ttgccgcccg tcgcggcgac catcgcatcc accgtccctt
    15961 cgccgccatc cgctatcggc aaacagatat agttcgcatc gggaaagagg gtcgaaaacc
    16021 cggctttaat tgcctgacaa catttttctg cactcaagct ctctttaaaa gagtctggcg
    16081 caatgacaat cttcattatc aatccttttt caacaaatta ttcgatgcgt tcccacggaa
    16141 taaggttatc cataagaaca gccagtaaaa tcccgaccaa taagccgtta ctgagcagag
    16201 gacgaagcgt taatggaagg tcttgcagat acacaggcgg taatgccatt aaaaatatgc
    16261 cgacaaataa cggcaatgcg agtcgataaa tattgcgagc ggtaaacgtt atttgctggc
    16321 taaacaccag cgcggaaaag agtaaaggta gataagaaac cagcatgacc gcactactca
    16381 cgggtaaagg gatactgcaa aacagtcgcg tgagtgccgg aaccagcgcc accagcaggc
    16441 aaataacgct gccataaata aacgaacgcc gcgtgtaatc gccagtctgg gttaataaac
    16501 caatggatga aacaaacggt gaaaatggaa ttactgccag cggcacggtt atcagtgtca
    16561 taaatccggt cgccacaaag ctacgacgat aacgcgtatt ccccgcgccc tgctgcggat
    16621 aaaaaacatc cgtgccccga atcgcaccgt aggtattgct gatattcacc agacctgtaa
    16681 tcaccgccgt cagaataatc cccggcgaca aagcaccgcc actgccgagc gggaaccact
    16741 gccagtgcaa ctcaccggag agcgaatgcg aagaaggaaa gcaaaagtac cacaacaacc
    16801 agccggttat ggtgccgacc agcaggccat aacgggcaaa acgttgcggc agaaagataa
    16861 tcatcgccag taccaggcac atcaccgcca ccgagagcgc gaacggcggt aactgaattt
    16921 taaaattcgg gtcggctatg ccaaacggca gcccgagcat acctttgaaa aagatagtgg
    16981 tcagctgcgc gcccagcatc aacataaaca agaccatcac cgacggcgta aataaccgcg
    17041 ccaggcgatg acctaatccg ctaaaaccaa tcaacatcgt cagcacgccg gagagtgcaa
    17101 tccccaccgc caggctggta gcgatatcgt tgatcggtgt cccgcgcgat gcttcgccaa
    17161 gggtgatagt taggatggtt ccccaccaca ggccccccgg cccttccata atagcgcgac
    17221 gatgaccgca aaacgcctga gcgaagcagg ccagtgcggt agcaagaaaa gcatattgcg
    17281 tgagcgtaag caggctactt tgcggcaact gaaaagcaga aagtagcgta ggaggaacca
    17341 caaccgtgtt gcaaaaaata aagaaaaacc actgaaatcc tgataacagg ctttcgcggc
    17401 taactgcaaa attgaacata aacacacctt aaccggagaa tgcccgccgc aaggacgggc
    17461 attgcagggg ccagattact gctgatgttt aaggataaat tgacctttcg gcgcaacagg
    17521 gaagccctgt tcgatgtcgt aaatcacatc accacgtaag atggttttcg tgatacgcgc
    17581 gccaatggta cggccaacat acgggctgac tttgtggcga tattccagat cgtcattggt
    17641 aagaacatag ctgctattcg gctgaatgaa gacgaagtcg gcatcttttc ctggggcgat
    17701 acggcctttt tgctgcagac caaaaatatc tgctgcgtta gtcgccatta atttgccgaa
    17761 cattggcaga gacattccgc gtttctgtac cgcttcatcg aacatcacgt ccatacagtt
    17821 ttgcagaccg gcaataccgc cccatgcttc catgatgttg ccggctttca tttccggagg
    17881 gcatggtgag tggtcggaaa ccaggcggtc gatttcaccg ttaaacagtt tttcccacat
    17941 gcctttctag ttttccagat cgcggatcgg cggtgaacac ttcgccagag taccgatttc
    18001 ttcgaactga tcggtatcca gtacgaagta atgcgggcag gattcacaag taacgtcctg
    18061 accttcctga cgtgcacgag tcacttcttc aacgccttcc gggctgctga tatggcaaac
    18121 gtgcagacgg caaccggcaa ctttcgccag atacagcacg cggcgaatcg cttccacttc
    18181 ggtaaatacc ggacgcgaag ccacatagtc atgggcagtt acgcgacctt cacgtttcgc
    18241 ttcttcgccc agtgcgtcac agatcagcgc gttttcgcag tgcaccagca ccggctgccc
    18301 cagttcgccc agcttctgcg cacctttgaa gaactgccag tcgtttacat cacggaagtc
    18361 gttgtcgata ccgcgatcgc cacaggtcgc aacgaagcat ttgaagccga caacgcccac
    18421 ttcatccagc tcatgcagac ggtcgatgtt gtaagacacc aggccaccga gttgtgccgc
    18481 atcgatagtc agcttgcctt tagcggcatc gaacttcagt tcaattgaag cgcggtcaac
    18541 cgttgcaggc agctggttga gcggcatttc gatcatggtg gtgataccac cttttgccgc
    18601 tgcgcgagta ccggtttcat aaccttccca gtggctacga cccggttcag aaatatgggt
    18661 gtgcgcatca accatgcccg gcgaaaccac cagaccagac gcatccataa cttcttttgc
    18721 atcgcccaga tcctgaccga tagcagcaat tttgccgcct ttaacggcga tatctacaac
    18781 gcgagcttcg ttttctaaaa taacggtgcc gtttttaatg attaaatcaa aagacataac
    18841 aaactccttg taaaatacag attggttttt aactcaagca aaggcgacga tgacgccgac
    18901 aaaccatgaa acacgcgata acggttccat taagggaata aacttaccgc caagagataa
    18961 aataacggcc accagagtta ctgaaaacgc ggtgaggtta aaaccgttat cgtaatattt
    19021 ataatcgcca ggtgcggtgt acagttcatc aagattaatt tgcccacgca tcaccacaaa
    19081 ataatgcgcc atcatgacac caattaccgg accaagcatt ccgccgataa tatcgaggaa
    19141 cagataaata ctgtcctgat tttccattaa tttccacggg cagatcagca ggctgataat
    19201 actggcaatc agtacgccgt ttttataggt cagttttgtc ggtgcaatgg cagcaatctg
    19261 ataaccagcc ggaataatat taccagtggc gttagtggag atagtcgtca tcagaataac
    19321 cagtaccgca aagaacgagg caaacaggct gtcccaacgc tgaacaatat ccagcacgtt
    19381 ccaggtatca gcgccgtaat gaatactggc tccggcaata atacataccc ccgcgaccgc
    19441 aaacagaata taggccacaa ctaaacccag cgtttgcccc agcgcttgct cacgaaacga
    19501 gtgggcgttt tgcgtaaagt cggatgcgct caccgccggt gccgcccaga ccgcaactac
    19561 cgcgttaatc accaccagga acaagaatcc actgttttct gctttctgaa taccgctcgg
    19621 aatgtagtca aagattggac cgagcccgac cagcgaaatc gcccaaatcg ccataccgcc
    19681 gaaaacgata tagatgcacg ggttaagaat ggcagtgaat ttatttaaaa ctttgccacc
    19741 gccaaaacct ataccaacgt tgaccagcca gaagagtaag aaagtaatta agcccggtag
    19801 agaaaggcct aacagagtga aatcaccacc gagagtcaaa aatcccggcc agattttgcc
    19861 aatcagaatc aagcaggcca gtgaccccgc gtaacattgc aggccaaacc acataatggc
    19921 agcaataccg cctcttaata atccgggaaa cagtgcgcca cgtacgccgt aagaagcacg
    19981 caggatcatg gcaaacggta cgccgtattt actgcccgca gcgccgttta ataccattac
    20041 cgcggcaatg aaaaaggcgc tgaggataat tgccagcata atactaaagg tagacaagcc
    20101 gagaataaaa aagccgccga ccatcacata attgggaacg ttatgaaccg aacccatcca
    20161 taaggtaaaa tagttaaatg ttttccaggt ccgctggctt tgcgttttcg gcaatagatc
    20221 ttcgctatag ccgcgttgct ggaatagttt tctctgatgt tccataaatc cctcttaatt
    20281 tcacgtatcg aataacgttg ttttaatcta gctatgagga aaaaataaaa taaaaatatt
    20341 atttttaata ctgaagcaac atctttggct gctttctttt ttaatattgc tggccgattc
    20401 attttttgat atcggccatt tttattgcgg gtatcaggcc agtttatggt tagccattaa
    20461 ttccagcgcc tgcactaacg cagagtgatc caactggctg ccaccgtttg ccgcgcaggt
    20521 attaaataac tcctggcagg tcgcagtgtt tggcaggttc agagccagtg ccttcgcact
    20581 ttgcagtgcc aggttgagat ctttctggtg cagcgcaatt ttgaaacctg gattaaaggt
    20641 gcgtttaatc atgcgctcac cgtgaacttc cagaatacgt gaggaagcaa agccgcccat
    20701 cagcgcctgg cgcacacgta ccgggtctgc accggctttt gaagcaaaca gcagagcttc
    20761 agagaccgct tcaatattga gcgctacgat aatctgattt gccactttgc aggtttgacc
    20821 gtcgccgtta ccgcccacaa gggtgatatt tttaccgagc agttcaaaca gcggtttaac
    20881 acgttcaaat accgcttcgt caccgccaac cataattgac aacgtccctt cacgcgcgcc
    20941 gatttcgccg ccggagactg gcgcatcgag ataatcacca cccagttcat tcacctgacg
    21001 agcgaaacgt ttagtttcaa tcggtgaaat ggagctcata tcaacaatgg ttttgccctt
    21061 cagcgaggct ttggtacaac cattttcacc aaacagaact tcttcaacct gaggtgtgtc
    21121 cggcaccata ataaaaatga tgtccgctgc ttccgttacc tggcgcgcgg tgtcgacatt
    21181 aacagcaccg agtgacagta attcatcagc aaccggtcca atggtcgtga catgtaattg
    21241 atgaccggca cgcgccagat taatggccat cggagtaccc ataatgccta agccaataaa
    21301 tcccagtttc ataattaacc tctttttaaa tgtcgctatt cctgaattca gacaacacga
    21361 tcccctgcca acttagcagc ctaaaaaagc attgtctgaa tagcgttacg ttttaacggt
    21421 acggatccat ccagcgtaaa ccggcttccg tggtggtttg gggtttatat tcacacccaa
    21481 cccaaccgtt gtagtcagaa ttttcgatta ccttaaagag ataatcataa ttaatttctc
    21541 cggtccccgg ttcgccgcga tgcggattat cggcaatttg caggtgacca attttatcag
    21601 cccattgcgt catagtgttg gttaattcac cttccatccg ctgcatgtga taaatgtcat
    21661 actgaatttt caaattgcag caaccaacat catcaatcaa tttcagcgcc tgccgagttc
    21721 cggtgagatg gaaaccagga atatcaaaat ggttaatagg ttcaatcagt aataaaatat
    21781 cttctttcat cagcatattc gcggcataac gcaggttttc tacaagcgtt gcgtgaatct
    21841 gttcactgct gaaaccagcc ggcgttttac cgaccagaca gttaatattt ttattaccca
    21901 gcgcacgggc ataacgaatc gctgctgcta cgccatcccg aaactcttct tcacggccag
    21961 gaatacaggc aataccgcgc tcccccgccg cccagtcacc cgccggtaaa ttgtgcagcg
    22021 tatgttcgag tttattactc gccagcacct gttttaattc ttcaatgtcg tagtcataag
    22081 gaaacataaa ttcaacgccg cgaaaaccac actgcgcagc tttctcaaaa cgggcgagaa
    22141 aatcatattc accaaataac atcgataaat tagcagagaa acgtaacatt atttatctcc
    22201 cttattcata gtgcatgaag caggtttcag tcggtgcatc cgctgcgtta tcggcgatat
    22261 cttcaaattc catgacgtta tccagttcgc tgcccatcga aatatttgtc acacgctcga
    22321 gaataacttc cacgactacc ggtacccgat attgcgccat taaggctttc gcctgttcaa
    22381 aggccggtgc aatatcttcc ggtttgaaga cgcgaatagc tttacaacct aaaccttccg
    22441 ctacttttac gtggtcgacg ccgtaaccgt tcacttcgct ggagttgata ttctcgaaag
    22501 cgagttgcac gcagtagtcc atgtcaaagg cgcgttgtga ctgacgaatc aggccgagat
    22561 aggcgttatt caccagcaca tggatgtacg gaatgttgaa ctgcgcgcca acggctaact
    22621 cttcaatcag gaactggaag tcgaagtcac cggaaatcgc caccacattg cgtttcggat
    22681 cagcggcaca aacccccagc gccgccggaa tcgtccagcc taacggacca gcctgaccac
    22741 agttgatcca gtggcggtct ttaaagacat gcagcatttg tgccgcagcg atttgtgaca
    22801 ggccaatggt ggtgacataa caaacatcgc gaccaaaggc tttgttcatc tcttcataca
    22861 cgcgctgcgg tttcaccggc acgttgtcga aatgggtttt gcgcagcaaa gtgcgtttgc
    22921 gctgctggca atcggcgacc cattctttac gacacggcag acgacccgct ttttgcatct
    22981 cctgcgccac ttcaaccagc agtgtcagcg ccgctttagc atcagagaca atgccgagat
    23041 ccggacacag cacgcggcca atttgcgtcg gctcaatatc gatatgaacg attttgcgcc
    23101 cttcggtgta tttctctacc gaaccggtat gacggttagc aaaacggtta ccgataccaa
    23161 acaccatgtc ggacgccagc aacgttgcgt taccgtaacg atgcgcggtt tgcagaccga
    23221 ccatcccggc catcagttca tgatcgtccg ggatacagcc ccagcccatc agcgttggga
    23281 tcaccggaac gctggtcagt tcagcaaact gttgtaacag tgcagctgcg tcagcattga
    23341 ttaccccgcc cccggcaaca atcaccggac gttcggcctg gattaacatt tctacagctt
    23401 tttcgatctg catacggctg gcagcaggtt tgtagaccgg cagcggttcg tacatgtcag
    23461 gatcaaactc gatttccgca acctgaacgt cgaacggtaa atccaccagt accggacccg
    23521 gacgaccaga acgcatcagg tgaaatgcct gttgcagcac gcgaggcacc agcgccgctt
    23581 cacgaactgt aaccgccatt ttgctgaccg gtttagcaat tgcttcaata tctacggcct
    23641 gaaaatcttc tttatgcaga cgggcgcgcg gtgcctggcc ggtaatgcac agaataggaa
    23701 tggaatcagc agaagcggaa tagagcgcgg tgatcatgtc cgtgcccgca ggaccggaag
    23761 tccccagaca tacgccgata ttccctgccg ttgcgcgggt ataaccttcc gccatgtgcg
    23821 aagcaccttc cacatgacgc gccagaatgt gacgaatacc gccgtgctta cgcatcgctg
    23881 agtagaacgg attgattgca gctcccggaa cgccgaaggc ggtggtgata ccttctttct
    23941 ccagcacata cattgccgcg tcaacggctc tcatttttgc cattttattc ctacccccat
    24001 taattggaaa aattttccaa ctttcaaatt ttgtcaaaaa aacccgctcc gcagagcggg
    24061 atgcgacaga cactatggat gtgctttcag tcccaacgcc gtgctgatat cgcgggcggt
    24121 gtctctgacc agctcaccct gactgacaaa acgatcttct gtcagtcttg atgaaggccc
    24181 ggagatggag atagcggcaa caacgctacc tacatcatcg taaattgctg aagctatgca
    24241 attcagacct acaacatgct cttctttatc tacggtatag cccagttcac gcgcttgttc
    24301 caggtccttc agcagggtgg gcatatccac aagcgtagtt ggcgtaaact gctgcaaacc
    24361 ggtttgcaga atgatgctca tcaactcctc ttccgccagc ggataaagca gcgctttgcc
    24421 cgcaccggaa gcatgcagtg gcagacgact gcccagtggc gcgcacatcc tgaccatcga
    24481 tttacactct aactgaccaa ttaataccgc ttcattgccg ttacggatcg cgacattgac
    24541 cgtttcgccg gaaagtaaca tcaggcggcg cataaacggc ccggcgacgg agaggacatc
    24601 gcggttatgg atgtacgccg caccgacgtt aaagacacct aatcctatat gccaccagcc
    24661 taactgactg tcctgataga caaaatccgc tgcctgtaaa accttcagca agcgaaaggt
    24721 cgtggagagc ggcaaatcca gattgagaga aatatcgcta accgacgaac ttcccccact
    24781 tttttccaga tattgcagaa tcgcaattcc ccgctctaat gcctgtgcgc ccttctgtgc
    24841 cacaggctcc gcctgtcctg gcctgccgcg ccgtctaact tccgtcatac ctacgttaac
    24901 tccgacctac aagtgagcat aaacaatgaa caattagttt atcttatgca agtcggttgc
    24961 aggcgtcacg caaaacagag ttcctgttca ggaatacttt caacatcaca gttgtcactg
    25021 ccgccgcgat cgatggtcag aaaatcggtg acgcgctgcc aggcgaaaag tgggtgatgc
    25081 cagacgttac gatggtaatt cactccctgt tcgccgttgg taataaacgc ccgcagggtt
    25141 gacaggtctg gtttgtcgtc acctaacgct acgaccacca caaacacctc acctttcatc
    25201 gggataaagg cctgagtacc cagcggatga cgttcgagtt cgtgaatggt cagcggcaga
    25261 ttcgccggtt gcgctcggtt aatgctgata agcgtacggt cttgctcaag aatctcaacc
    25321 agcgccaaat cgtggtaacg ctccaccagg ccattgttaa tatggaaaaa atcccgttgc
    25381 tgcgtttcga ttacgtcgcc ataagcacta aaggcttcct gacttaacgg taatacctga
    25441 agtttcatct ttttgtcctt gtaacataat tccgttcaac cagcgccgac cggtcagctt
    25501 ggtttcaata acaggcaatc taaaacctta agagaacaac atcaacacaa aacttggaat
    25561 atttttccaa aactagattt agtccacaaa ttgaacaggg ttatcatgta aaaatactga
    25621 taaatcagag gataagaaaa tcacgcaact tgcggattta aaaggggata cctgaagatg
    25681 ttcgatccag aaaccttgcg gactttcatt gcggttgctg aaacaggaag tttttcaaaa
    25741 gcggcagaac gattatgtaa aaccacggcg acgatcagtt atcgcattaa acttctggaa
    25801 gagaataccg gagtggcgct gttttttcgc acgactcgta gcgtaacgtt gacagcggct
    25861 ggcgagcatc tactttccca ggccagagac tggctgagct ggctggaaag tatgcccagc
    25921 gagctgcaac aggtgaatga tggcgtggaa cgccaggtga atattgtcat caacaacctg
    25981 ctctacaacc cccaggccgt cgcccagttg ctggcgtggc tgaatgaacg ttaccccttt
    26041 acccagtttc acatctcccg acaaatctat atgggcgtct gggactcgct attgtacgaa
    26101 ggtttttcac tggctatcgg cgtcacggga accgaagcgc tggcaaatac ctttagtctt
    26161 gatcccttag gatcggtgca atggcgattt gtcatggcgg cggatcatcc gctggcgaac
    26221 gttgaagagc cgctaacaga agcgcagttg cggcgctttc cggcggtcaa tattgaagac
    26281 agcgcccgca ccttaaccaa acgcgtcgcc tggcgattgc cagggcaaaa agagattatt
    26341 gttcctgata tggaaacgaa aatcgccgcc catctggcgg gcgttggcat tggttttttg
    26401 ccaaaatcgc tttgccagtc aatgatcgat aatcaacaac tggtcagccg ggtaatccca
    26461 acgatgcgcc ctccttcgcc attgagtctg gcatggcgca aatttggcag cggcaaagcg
    26521 gtagaagata ttgtgacctt gtttacccag cgcaggccgg aaatcagcgg atttttagaa
    26581 attttcggca acccacgcag ttaaaaatca agtttccttg cgcgttggcg cgtaaaatag
    26641 cgcgcaagct gatttgaggt tcacttttaa ctatgcaaga gagacacacg gaacaggact
    26701 atcgtgccct gctgattgct gatacgccca ttattgatgt tcgcgcccct atcgagtttg
    26761 agcaaggcgc aatgcccgcc gctatcaatc tgccgttaat gaataacgat gaacgcgccg
    26821 ccgttggcac ctgctataaa cagcaaggct cagacgcagc gctggcgctg ggacataaac
    26881 tggttgcggg tgaaattcgt cagcagcgca tggacgcctg gcgggcagcc tgcctgcaaa
    26941 atccgcaagg tattctctgc tgcgcccgtg gcggtcagcg ctcacatatt gtgcaaagct
    27001 ggttgcatgc agcggggatt gattatccgc tggtggaagg cggttataag gcactgcgcc
    27061 agaccgcgat tcaggcgact attgaactgg cacaaaaacc gatagtgctg attggcggtt
    27121 gtaccggcag cggtaaaacg ctgttagtgc agcaacagcc gaacggtgtt gatctggaag
    27181 ggttggcgcg tcatcgcggt tcggcgtttg gtcgcacgtt acaaccacaa cttagccagg
    27241 cgagttttga aaacctgctg gctgccgaaa tgctaaaaac cgacgcccgt cagaatttgc
    27301 gcctgtgggt gctggaagac gaaagccgga tgatcggttc gaatcacctg ccggaatgcc
    27361 tgcgcgagcg aatgactcag gcggcgattg cggtggtaga agatccgttt gagatccgtc
    27421 ttgagcgcct gaacgaagag tatttcttgc gtatgcatca tgattttacc cacgcgtacg
    27481 gcgacgaaca gggctggcag gaatattgcg aatacctgca tcacggactt tcggctatta
    27541 agcgtcggct ggggctacag cgatataacg aactggctgc aaggctggat gcagcactga
    27601 caacgcaact caccaccggc agcaccgacg gtcatctggc ctggctggtg ccgttacttg
    27661 aagaatatta cgacccgatg tatcgctatc agcttgagaa aaaagcggaa aaagttgtct
    27721 ttcgcggtga gtgggcggaa gtggcggaat gggttaaggc gcagtaatga gggacgtatt
    27781 tgtattagtc gcgacttgat ctgacacatg gccttgaaag gttgagagtt accggttttg
    27841 atatgagtgt cgaatcctta tccaaaacaa gaggtaactc tcatgcttca tactgccaat
    27901 cccgtcatca aacacaaagc cggtttgctc aatctggctg aagaactcag caacgtatca
    27961 aaagcctgta aaatcatggg cgtttcgcgt gatacgtttt atcgttaccg cgaactggtc
    28021 gctgaaggcg gtgtggatgc gcagattaat cgtagtcgcc gcgctcctaa ccttaagaac
    28081 cgtaccgatg aggcaactga acaggctgtt gttgattacg ccgtcgcttt cccgacacat
    28141 ggtcagcacc gggccagcaa cgaactgcgt aaacaggggg tttttatctc cgacagtggt
    28201 gtccgttctg tctggttact ccataacctt gagaatctca aaagacgata ctgaacaaat
    28261 tttatcaggt gacgttccgt aaaaagttgt atggagttct cgatgcagtc ggcgcgacta
    28321 tcagtacccc cgctgaaccc tggactatcg ggccgccgta ctggggcatg tcaccctggc
    28381 gtgccgctgg ttttccgcta atggtttatt tcctctttct ttcccggcaa gaatttattt
    28441 taatccggaa ggattattta ttttcagata gggactcaca ttaaacacgt aacattttaa
    28501 ttaatgcatg gctgaatatg tattacacaa tgtcagatat cgtaaaagac cagtgcaatt
    28561 ctatgtaaac tctctgactg ttcattttat ttgttgtttc agggtcggaa ctcaccctca
    28621 ataaacaact gaatacgagt agatattcat cgtctgagct atatggcttt acacaatagc
    28681 cgacatgtct tttctcagtc aggaatttta cttttcctca ctacgaagtt gacgacttcg
    28741 atatgggata gactcttaat tcaagcaatt actctgcaca agcatcaggc aatccagctc
    28801 caatatttgt tataaaataa tgcgtaacat taatttcacc aacaaaaaaa ggtttacacc
    28861 atccccttgc cctggtatct taccatattt acaccacact ttttttgtat ctggatcatt
    28921 ctttgatacg attttcgcca cttgttccct ggccctcact gtcaatgttt tcttaaacct
    28981 ccccccatca tcatttgtta gggaaaaata agaaaaaaat aatcatcttc tacatttgtg
    29041 tatattgata tatccaccgc tacgtgcgga aaaaaatctg ggagccatgt aaaaactcca
    29101 tcattttctg gtattgccat atagtttatg taatgtttac ttacaagag
//
LOCUS       DAZKIF010000055        28760 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZKIF010000055 DAZKIF010000000
VERSION     DAZKIF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.055"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(46..1014)
                     /gene="astE"
                     /locus_tag="ACHOTW_002359"
     CDS             complement(46..1014)
                     /gene="astE"
                     /locus_tag="ACHOTW_002359"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP9844459.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1007..2350)
                     /gene="astB"
                     /locus_tag="ACHOTW_002360"
     CDS             complement(1007..2350)
                     /gene="astB"
                     /locus_tag="ACHOTW_002360"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP9844460.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(2347..3825)
                     /gene="astD"
                     /locus_tag="ACHOTW_002361"
     CDS             complement(2347..3825)
                     /gene="astD"
                     /locus_tag="ACHOTW_002361"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP9844461.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(3822..4856)
                     /gene="astA"
                     /locus_tag="ACHOTW_002362"
     CDS             complement(3822..4856)
                     /gene="astA"
                     /locus_tag="ACHOTW_002362"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP9844462.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(4853..6073)
                     /gene="astC"
                     /locus_tag="ACHOTW_002363"
     CDS             complement(4853..6073)
                     /gene="astC"
                     /locus_tag="ACHOTW_002363"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP9844463.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            6519..7325
                     /gene="xthA"
                     /locus_tag="ACHOTW_002364"
     CDS             6519..7325
                     /gene="xthA"
                     /locus_tag="ACHOTW_002364"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9844464.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            7492..8202
                     /gene="ydjX"
                     /locus_tag="ACHOTW_002365"
     CDS             7492..8202
                     /gene="ydjX"
                     /locus_tag="ACHOTW_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9844465.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            8207..8884
                     /gene="ydjY"
                     /locus_tag="ACHOTW_002366"
     CDS             8207..8884
                     /gene="ydjY"
                     /locus_tag="ACHOTW_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP9844466.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            8899..9606
                     /gene="ydjZ"
                     /locus_tag="ACHOTW_002367"
     CDS             8899..9606
                     /gene="ydjZ"
                     /locus_tag="ACHOTW_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9844467.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            9606..10154
                     /gene="ynjA"
                     /locus_tag="ACHOTW_002368"
     CDS             9606..10154
                     /gene="ynjA"
                     /locus_tag="ACHOTW_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9844468.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10164..11330
                     /gene="ynjB"
                     /locus_tag="ACHOTW_002369"
     CDS             10164..11330
                     /gene="ynjB"
                     /locus_tag="ACHOTW_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9844469.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            11303..12838
                     /gene="ynjC"
                     /locus_tag="ACHOTW_002370"
     CDS             11303..12838
                     /gene="ynjC"
                     /locus_tag="ACHOTW_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9844470.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            12838..13491
                     /gene="ynjD"
                     /locus_tag="ACHOTW_002371"
     CDS             12838..13491
                     /gene="ynjD"
                     /locus_tag="ACHOTW_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9844471.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            13558..14865
                     /gene="ynjE"
                     /locus_tag="ACHOTW_002372"
     CDS             13558..14865
                     /gene="ynjE"
                     /locus_tag="ACHOTW_002372"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP9844472.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(14874..15494)
                     /gene="ynjF"
                     /locus_tag="ACHOTW_002373"
     CDS             complement(14874..15494)
                     /gene="ynjF"
                     /locus_tag="ACHOTW_002373"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9844473.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            15581..15988
                     /gene="nudG"
                     /locus_tag="ACHOTW_002374"
     CDS             15581..15988
                     /gene="nudG"
                     /locus_tag="ACHOTW_002374"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP9844474.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(15954..16226)
                     /gene="ynjH"
                     /locus_tag="ACHOTW_002375"
     CDS             complement(15954..16226)
                     /gene="ynjH"
                     /locus_tag="ACHOTW_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFP9844475.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            16462..17805
                     /gene="gdhA"
                     /locus_tag="ACHOTW_002376"
     CDS             16462..17805
                     /gene="gdhA"
                     /locus_tag="ACHOTW_002376"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9844476.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(17922..18962)
                     /gene="ynjI"
                     /locus_tag="ACHOTW_002377"
     CDS             complement(17922..18962)
                     /gene="ynjI"
                     /locus_tag="ACHOTW_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9844477.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKTMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEEQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(19090..21051)
                     /gene="topB"
                     /locus_tag="ACHOTW_002378"
     CDS             complement(19090..21051)
                     /gene="topB"
                     /locus_tag="ACHOTW_002378"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9844478.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(21056..22099)
                     /gene="selD"
                     /locus_tag="ACHOTW_002379"
     CDS             complement(21056..22099)
                     /gene="selD"
                     /locus_tag="ACHOTW_002379"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP9844479.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(22216..22767)
                     /gene="ydjA"
                     /locus_tag="ACHOTW_002380"
     CDS             complement(22216..22767)
                     /gene="ydjA"
                     /locus_tag="ACHOTW_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP9844480.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            22928..24784
                     /gene="sppA"
                     /locus_tag="ACHOTW_002381"
     CDS             22928..24784
                     /gene="sppA"
                     /locus_tag="ACHOTW_002381"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9844481.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            24900..25610
                     /locus_tag="ACHOTW_002382"
     CDS             24900..25610
                     /locus_tag="ACHOTW_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFP9844482.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            25742..26758
                     /gene="ansA"
                     /locus_tag="ACHOTW_002383"
     CDS             25742..26758
                     /gene="ansA"
                     /locus_tag="ACHOTW_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9844483.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            26769..27410
                     /gene="pncA"
                     /locus_tag="ACHOTW_002384"
     CDS             26769..27410
                     /gene="pncA"
                     /locus_tag="ACHOTW_002384"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP9844484.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(27503..>28760)
                     /gene="ydjE"
                     /locus_tag="ACHOTW_002385"
     CDS             complement(27503..>28760)
                     /gene="ydjE"
                     /locus_tag="ACHOTW_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9844485.1"
                     /translation="GFLSYSGNVVLAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGG
                     FIGDYFGRRRAFRINLLIVGIAATGAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFT
                     EFIPATVRGKWSARLSFVGNWSPMLSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSG
                     KYFIESPRWLAGKGQIAGAECQLREVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFW
                     LLFKGEMLRRTLVAITVLIAMNISLYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAP
                     VGIFIAALIIDHFPRRLFGSTLLIIIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVC
                     FASAVYIPELWPTHLRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFMVLGVML
                     LLCALVLSIFGIETRKVSLEEISEVN"
BASE COUNT         6922 a         7707 c         7308 g         6823 t
ORIGIN      
        1 aagaaggtaa cttaataccc gcagaaagat ttctgcgggt aagtattagc ttattttttc
       61 gagcattaat cccgcgcgta atcccagcgc taccaacgga ttagggaata acacatactc
      121 tacatcatgg gttacggtaa aacgttcctc accgtcctgc gccagcaacg ttcctttctt
      181 aaacggcata aaattcagcg tgtcacttgc catatgcatt tcgaaggacg gcgagtggcg
      241 agtaatttgc gaaaccaccc gataacggag cggcggtgtt ctcacgatac cgacactctc
      301 accagatagc agcgcagcga ttgcgctggc agttactgca aactggcgaa gatcgttttg
      361 cccaaagggc aacgctttgc caagttccag cgtacaggcc agcgcgccaa aatgtctggc
      421 gctgaaatgg gtaaacgtac caccaggctc ctgatggaac accagcgcct ccagccccgc
      481 cgcacccagc cacgtcagga atttctcgtc ccagggaatg tcgcgttgcg gtaatacacc
      541 gaactgcgga tgcaaggagc cacgaattgc ggtatgtaga tcaaggtgcc agcgcacaga
      601 ttctttgccc tggtcataaa aatcttccag gcactgttcc agttcgcgcg cccgacaggt
      661 ttccccgctt tcagcaaaca gctgccaacg accgccaaac attcggttca tatcgctatg
      721 gcaataacgt ttcccttgct tcagcgcagg aggattcccg aggatcacca gcaaccgcca
      781 ccgtaacggg atctcgccgt gagatatcgc gccaagcaat gcatcaagca tctccacagg
      841 tgccgtctca ttaccgtgga tccccgcaga aatcaccaga gcgccttgcg gtggcgttaa
      901 tggcgtcagt tccagcacac catcgcccag ccagcgccag cgaacgccgt tgatttctcg
      961 ctcggtgatg acaggttttt tactcgttaa ggtcagagca agaaaattat ccattgcccc
     1021 ctccctcgcg ctggaacgga taaaccgaac cgagattcag taattggctc aatacatcca
     1081 gtgcttcccg cccttcgcgc agcaattgcg ggtcggccag atcggcagca gtaagacgat
     1141 cgcggtagta acgatccacc cagtcattga gcgcattaaa caaggtatcg ttcatcatca
     1201 ccgccggatt caccgcccgg cgttcttctt gtgtcaatac cacccgcaac cgcaggcacg
     1261 ccgggccacc gccattcgcc atgctttccc gtaaatcaaa gacttttagt tcgctaatcg
     1321 ggttgtcagc ggcaaggagt tcattgagat aaccccatac tccggcgtgt tcccgacact
     1381 cctgaggcag caccaacatc atggaaccat catcgcggct caacagttgg ctgttaaaca
     1441 gatatgtaga caccgcatcc gacacggaaa cctgagttgc cggaacttct atcgccataa
     1501 aaccgttcac ccgcgcacgc aggtttgcca gtaactgtga ctggcgagcg aacgcctgtt
     1561 ggtggcaaaa cagcacctgg cggttactca cggcaatcac gtcattatga aaaacgccct
     1621 ggtcgataac gtccgggttt tgctgggcga aaatcacctg ttggggattc acctgattca
     1681 gccttgccac cgcctcgctg gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg
     1741 tatcattgcc ctcttctcgc ccgtagacaa aaagttgcat acccggttca ccgtaatgac
     1801 cgccgagacg attgtggttt gccgccccct catcaccgag caaggctacc tgcggcaacg
     1861 ccgaatggac actaaatttc tcttcgtcgt taaaaatcgc ttttaacagc gattcagtga
     1921 cgggcgcttc cagcgaacgg tgaaatttat tgttcaggtt agcaacggtg agatgcactt
     1981 tgccatccag cgtatcggca gatggcgcga tcgttgccgc attggctacc cacattggcg
     2041 aagcggaact gacgctggaa agccagtgcg gtgcctggcg tacaaccttt tccagtacct
     2101 gctcatcgct accactgaat cccaactgac gcagcaccgg aataaacgga cgctcgtgag
     2161 gcgggatcac ggcctggggg aatcccgcat cggcaagggt tttcattttc agtaagccct
     2221 gttttgccgc cagtcgcggg ttagagacct gaaaacggtg acgggtagag gctttattac
     2281 caaacgacag gcccgcgtaa tgatgcgtca gccctaccag cccgtcgaaa ttaacttccc
     2341 aggcgttcat cgcaccacct catcggaaaa atccagcccg gggttaagcg ttgcgggcaa
     2401 agttaacgag tccgactcca ggctcgccat aggccatgcg cagtaatctg cggcatacca
     2461 ggcgctgggg cgatggttac cggatgcacc aatgccgccg aatggcgcgg tactggcagc
     2521 accagtaagc ggtttgttcc agttaacaat ccccgcccgc gcctccagca acagttgatc
     2581 gaatttttcc cgctcggggg aaaccagacc gcaagagagt ccgaagcgag tgttattcgc
     2641 cattcgaatc gcttcctcga aagaatcata acgccagacg cgcaataacg gtccgaacac
     2701 ctcttcatct ggtacgccag caacgcctgt catttcaatg atccccggcg tcagcaacga
     2761 tgtccctgct tgtaataagc gcggcgcaag cagggttcgt ccacccatcg cttccagttg
     2821 ctgccaggca gtaaacacct gctgtgcggc ctgttcagaa atcagcccgc caataaacgg
     2881 ctgcggttcg tcatcccagt tgcccggcgt taatcgctgg ctgacggcaa ccagacgagc
     2941 aagaaatgca tcgccctgcg ccccgctttt cagcaataca cggcgggcgc aggtgcagcg
     3001 ttgcccggct gtaacaaacg ccgactgaat ggtcagatgg acagccgcgt cgatatccgc
     3061 cacctcatcg ataatcagcg ggttattgcc gcccatctca agggcgagaa ttttctccgg
     3121 ctgaccggag agctggcgat gcagctggta gccggtattg gcgctgccgg taaacagcaa
     3181 gccgtcgaga tcctccagcg cactcagcgc ctgacccgtt tcacacccgc cctgcaccag
     3241 gttcagcacg cctggcggca agccagcctg ctgccataaa cgcattaccg cttcgccact
     3301 ccacggtgtc agttcgctgg gtttaaagat aatggtgtta cctgccagca atgcgggaac
     3361 gatatgtccg ttcggcaaat gaccagggaa attatacggc ccaaacaccg ccagcacgcc
     3421 gtgcgggcga tgtcgcaggc tcgccgcacc gtccggcatt tcactacgct gctcgccggt
     3481 acgaacgtga tacgccttaa ttgatatcgc gattttattg atcatcgccg tcaattcggt
     3541 tgccgcctcc cagcgcggct tgccggtttc tcgggcaatg attgcggtta attcgccttt
     3601 attcctttcc agcaatccgg caaagcgttc gacaacggcc tgacgttcag caaatgagag
     3661 ccgcgcccag cgcggaaacg ccgcacgggc tgcccgacaa gcctgcccga cctgagcggc
     3721 atcggcatca ttgccttgcc ataacacctc gcccgatacc ggattacgct tcacacgcga
     3781 tgcaccctgg cccgttatcc agtcaccgtt aatccataaa gtcatgctgt tttctcctct
     3841 gcgcacaggc gcaccagacg aacgcgatcc cctgcatggc atttgagggc atccagttgt
     3901 gcggcggtta aaatcaaacg ctcggttgcc ggatcggtac gcaccagcac cacgcggaaa
     3961 tggtgataat tttcattggc gaccaggcag gccggaaaat cgccctgcgc aggctgccct
     4021 tctgccactt ccaccagccg acttttacgg atggcgcgca cgcggtcgat gtcacactca
     4081 agcgtcggcc caccgtcgaa gatgtcgata tagttgcggt agcgaaaacc ttctttctcc
     4141 agcaccgcgc gggcaggcgc ggtttgcgga tgtacctgac cgatgacgtc ctgggcttcc
     4201 tgggataaaa agtgcgtata gatcggatgt ttcggcatca gttctgcaat aaatgccttt
     4261 tgcccggtgc cgcagagaaa atcggcgcgg ctaaaatcca tcgaaaagaa gcgtttaccg
     4321 aggctttgcc agaacggtga atagccgtgt tcgtcaatca ccccgcgcat ttcagcaacc
     4381 actttgtcat taaacttgtc gcgaaaagcc gccataaaca taaagcgcga tttcgacagc
     4441 aaatagccgt tgccctcttt gcgccagtcc gggtcgagaa acagcgtgca cagctcgctg
     4501 ctgccggtgt gatcgttact gagaaacaac gtcggcaatg cgttatagac attcagctct
     4561 tttgaggcgt gaaccaacgt gccgacgcga tagttgtacc agggatcgtt cagcccaact
     4621 gccacctcaa tggcgcaaat ccccgccacg gtgcctgtct cgctatcttc cagtacaaac
     4681 acataaccct gctcactttt gggcagttca ccttgccagg ttttgattgc cctttcgata
     4741 cgcgccgaaa gcgtggcttc attggcggga agcgacgtca ggccgccgcc cgttttgctg
     4801 gcaagctgca tcagcgccga gacatctgag cgctcaacgg gacggatgac catcatgatg
     4861 aacctcggct aacaaagtgt tcgcaagcaa ctgcaaagcg atccagtccg gtcgtcacct
     4921 cttcttcgct gacattgagc gcaggcgcaa aacgcaccac gttgccaccc gcaatcagta
     4981 ccatcacgcc tgctttcgcc gcttcctgag agatctgttt cgcttgcccg gcgtaatcgg
     5041 cattcagtac acagccaatc agcaaaccta agccgcgaac ttcattgaac aaaccatagc
     5101 ggtgattaat aatattaaga cgctcaacaa accagtcgtg acgttgctta accccgttaa
     5161 gcatctctgg cgtattgatg agctccagca ctttgcctgc caccgccgag gccagcggat
     5221 tgccgccata ggtggtgcca tgagtgccaa cggtcatcac gctggcgcac tcttcggttg
     5281 ctaacaacgc accgaccggg aaaccgccgc ccagagcttt ggcggtagtt aacagatcag
     5341 gcgtcacgcc gtagtgcata taggcataca gttccccggt acgcccgacg ccggtttgta
     5401 cttcatcaaa aatcaacagc gcattgtggc gatcacacaa ttcacgcaga ccttgtaaaa
     5461 atgcgttgct ggctggcacc acaccgcctt caccctggat gggttcgaca atcaccgcac
     5521 aggtagagtc gtcaatcagc gcgctggcag agttaatatc gttatatgca gcatgacgga
     5581 tatctggtgg cagtggcgca aaatcctgtg aataggctgg ctgcccaccc gcactaacag
     5641 taaacagcgt gcgaccatga aacgcatttt taaacgccac gataccgctc ttatggctgc
     5701 cgtagcggtc gtgagcgaat ttacgcgcca gttttagcgc cgcttcgttg gcttccgcac
     5761 cggagttaca aaagaaaacg cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc
     5821 gcagtaccga ctcgttggtg taaccgttgc cggtatgcca gaacttactc gcctgttcgt
     5881 tcagcgcctc acgcagttcc ggatgcgcat ggcccagcgc gttcaccgca atgccacccg
     5941 cgaagtcgat atactctttc ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg
     6001 gtataaacgg tgccggagcg taaacaggta tcatccattc atcaaagttt tcacgcgtaa
     6061 ttggctgaga catagcgacc tctacagtaa atagctcgtt atttatatgt taataataag
     6121 taatgttttc gctgtaaatg tagattgcag ggttcgtgcc agccagtgat gaaagtgcat
     6181 aaacggcgga ggctaactgg aaatcaagga gttataacca aaccatatgc atttaaagtg
     6241 catattaagt gaatacgttt gcgatgtggg tgaataaaaa gaataaaaaa cgcaatgtta
     6301 tgcagaagta aaatataatt ctggaattgt gatcattgac gaaatttact ggaaattact
     6361 gcgccattct gacgcagcgc gcaccaaaag cgggcatttt ttgcgccatc gttgacatca
     6421 ttaacaacca tcgatcaaat cacttaacaa caggcggtaa gcaaagcgaa attctgctac
     6481 catccacgca ctctttatct gaataaatgg cagcgactat gaaatttgtc tcttttaata
     6541 tcaatggcct gcgcgccaga ccccaccagc ttgaagccat cgtcgaaaag caccaaccgg
     6601 atgtgattgg cctgcaggag acaaaagttc atgacgatat gtttcccctc gaagaggtgg
     6661 cgaagctcgg ctacaacgtg ttttatcacg ggcagaaagg ccattatggc gtggcgctgc
     6721 tgaccaaaga gacgccgatt gccgtgcgtc gcggctttcc cggtgacgaa gaagaggcgc
     6781 agcggcggat tattatggcg gaaatcccct cgccgctggg caatgtcacc gtgatcaacg
     6841 gttacttccc gcagggtgaa agccgcgacc atccgataaa attcccggca aaggcgcagt
     6901 tttatcagaa tctgcaaaac tacctggaaa ccgaactcaa gcgtgaaaat ccggtgctga
     6961 ttatgggcga tatgaatatc agtcctggcg acctggatat tggcattggc gaagagaacc
     7021 gtaagcgctg gctgcgtacc ggaaaatgtt ctttccttcc ggaggaacgc gaatggatgg
     7081 agagactgat gagctgggga ttggttgata ccttccgcca tgcgaatccg caaacagcag
     7141 atcgtttctc atggtttgat taccgctcaa aaggttttga cgataaccgt ggtctgcgca
     7201 tcgacctgct gctcgccagc caaccgctgg cagaatgttg cgtagaaacc ggcatcgact
     7261 atgaaatccg cagcatggaa aaaccgtccg atcacgcccc cgtctgggcg accttccgcc
     7321 gctaatttag cagctctcct ggctcaaact gggtcaggag aattaacctt gagaaaaatc
     7381 aacaaactgt cagtaatgat ttgttgcctg ccgtcctttg ttataccgtc actgcgtttt
     7441 tagttgtctg accacttctc tattatcaag tttgatatag gaaactccac gatgaacgct
     7501 gagcgtaaat ttctttttgc ctgtcttatt tttgcgctgg ccatttacgc tatccacgct
     7561 ttcggtttat tcgatctgct caccgattta ccccacttac agacactcat tcgccagagc
     7621 ggattgttcg gttatagcct ctatattctg ttattcatca ttgccaccct ctttctgtta
     7681 ccaggaagca tattggtgat cgcgggagga atagtttttg gcccgttctt agggacacta
     7741 ctctcattaa ttgccgccac gctggcctcc tcgtgctcat tcctgctggc gcgctggatg
     7801 ggacgtgatt tactgctgaa atacgttggc catagccata ccttccaggc cattgaaaaa
     7861 ggcattgcgc gtaacggtat tgattttctt attctgaccc gcttaatccc gttgtttcct
     7921 tacaatattc aaaattacgc ttacggatta accacaatcg ccttctggcc ttataccctt
     7981 atttcggcac tcacgaccct gcccggtatt gttatttata ccgtgatggc aagcgatctc
     8041 gccaatgaag gcattacgct gcgctttatt ttacaactct gtctggcggg cctggcgctg
     8101 tttattctcg tccagctcgc aaaactctac gcccgacaca aacatgtgga tctgtctgct
     8161 tcgcgccgca acccacttac tcacccaaaa aatgaaggat agaacgatgt tgcaacatta
     8221 ttcagtgtca tggaaaaaag gactgactgc actctgctta ctggctgttg ctgggcttag
     8281 cggctgcgat caaaaagaga atgcggcggc aaaagtggaa tacgacggac tttcgaacag
     8341 ccaaccgctg cgcgtcgatg ccaataacca tacggtaacc atgctggtgc aaattaatgg
     8401 tcgtttcctc accgacgaca ctcgtcacgg tattgtgttt aaagatggct ccaacggaca
     8461 taaatcgctg tttatgggtt atgcgacccc gaaagcattt tatgaagccc tgaaagaggc
     8521 aggtggtaca ccgggcgaaa acatgacgat ggataataaa gaaacgactc atgtcacagg
     8581 cagcaaactg gatatttcgg tcaactggca aggggcggca aaaacgtatt ccttcgatga
     8641 agtgattgtt gacagtaatg gcaagaaact ggacatgcgc tttggcggta atttaacggc
     8701 agcagaagag aagaaaacgg gttgcctggt gtgtctggat agctgcccgg tcggcatcgt
     8761 cagcaatgca acatacactt atggtgcggt tgaaaaacga ggtgaagtta aattcaaagg
     8821 caatgcctcg gttctcccgg cgaataacac gctggcaacg gttaccttta aaatcaccga
     8881 ataaagccag gataaaggat gatgaagatg caatcgcgta aaatctggta ctaccgtata
     8941 accctcatca tcctgttgtt cgctatgctg ctggcatggg cgctgcttcc cggcgtccat
     9001 gagtttatca atcgcagcgt tgcggcgttt gccgccgtgg accaacaggg tatagaacgc
     9061 tttattcagt cttacggcgc actggcggca gttgtctcgt tcttgttgat gattttacag
     9121 gccattgctg caccgctacc tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc
     9181 gcgttctggg gcggctcgct gtcgtggacc agttcgatgg ccggcgcggc actgtgcttt
     9241 tttatcgcca gagtgatggg ccgcgaagtg gtggaaaaat taaccggcaa aaccgtactt
     9301 gacagtatgg acggcttttt cactcgctac ggcaaacaca ccatcctggt ctgtcggtta
     9361 ttgccttttg tccctttcga tccaatcagc tatgctgccg gtttgacttc aatacgtttt
     9421 cgctcgtttt ttatcgccac cgggcttggt cagttaccgg cgactattgt ttattcctgg
     9481 gcgggcagca tgttaacagg cggtaccttc tggtttgtca ccggactgtt tattctgttt
     9541 gccctgaccg tggtgatttt tatggcgaag aaaatatggc ttgagcgcca gaagaggaat
     9601 gcctgatggg tttaccgccg cttagcaaaa ttcctttcat tttacgtccg caggcgtggc
     9661 tgcatcgtcg ccattacggc gaggtgctaa gccccattcg ctggtggggg cggatcccgt
     9721 ttatctttta tctggtgtcg atgtttgttg gctggctgga gcgcaaacgc tcaccgctcg
     9781 atccggtagt acgatcgctt gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt
     9841 gtgtggatat caccagtatg aaagtcgccg agcgcaccgg cagcagcgat aaactgctgg
     9901 cagtggctga ctggcggcaa agcccgctct ttagcgatga agaacggctg gcgctggagt
     9961 acgccgaagc cgcaagcgta acgccgccaa cggtcgatga tgccctgcgt acccgactgg
    10021 ctgcgcattt tgacgctcag gagctcaccg aactgacggc attgatcggc ctgcaaaatc
    10081 tgtcagcccg ttttaattct gccatggaca ttcccgctca ggggctgtgc cgtattccgg
    10141 aaaaacgttc ttaaggagag atgatgcgcc attgtgggtg gttgctggga ttgttatcgc
    10201 tgttttctct ggcaacacat gccagtgact ggcaagaaat taaaaatgag gccaaagggc
    10261 aaactgtctg gtttaacgcc tggggcggcg ataccgcaat taaccgctat ctcgactggg
    10321 ttagcggcga gatgaaaacc cattacgcta taaacctgaa gattgttcgt ctggcggatg
    10381 ccgcagacgc ggtgaagcgc attcagaccg aagctgctgc cggacgtaaa acgggcggct
    10441 cggtggatct gctctgggtg aacggcgaaa acttccgcac cttaaaagag gccaatttac
    10501 tgcaaaccga ctgggcagag actctgccca actggcgcta tgtcgacaca cagctgccgg
    10561 tgcgggaaga tttttcagtg cctacagaag gggctgaatc gccctggggg ggcgcacaac
    10621 tgacatttat cgcccgccgc gatgttacgc cacagccacc acaaacgccg caagccttac
    10681 tggagtttgc taaagccaat cccggcacgg ttacctatcc gcgcccaccg gactttaccg
    10741 gcacggcgtt tcttgaacag ttgctgatta tgctgacgcc cgatcccgcc gcattaaatg
    10801 aagcgccgaa cgatgcgact ttcgcccgtg tcactgctcc cttgtggcaa tatcttgatg
    10861 cgctgcatcc gtatttgtgg cgcgaaggaa aggatttccc gccttcgccc gcgcggatgg
    10921 atgctctgct gaaagccgga acatttcgcc tgtcgctgac ctttaacccc gcgcatgcgc
    10981 agcaaaaaat cgccagcggc gatttgcctg caagcagtta cagttttggc tttcgcgagg
    11041 ggatgattgg caacgtgcat ttcgtcacca ttcctgccaa cgcgaatgcc agtgctgcgg
    11101 cgaaggtagt tgccaatttc ttgctctcac ccgatgcgca attgcgtaaa gcagatcccg
    11161 ctgtctgggg cgatccttct gttctcgatc cgcaaaaact gcctgatggg cagcgcgaaa
    11221 cattgcaatc aagaatgccg caagatctgc cgccggtact ggctgaaccg cacgcaggtt
    11281 gggtaaatgc gctggaacaa gaatggctac accgttacgg tacgcattaa tctttttgct
    11341 gtgggcgatg gtggcggtga tttatgcacc gctgatcccg gcagctctca cgctgatatc
    11401 gcctgctttg tcgttgacac actggcaggc gttatttgcc gatccacagt taccgcaggc
    11461 attactggca acgctggtgt cgacaaccat cgcggcggtc ggggcgttgt tgattgccct
    11521 actggtgatt gtggcgctgt ggcctgggcc gaaatggcag cgtatgtgca cccgtctgcc
    11581 gtggctgctc gccattcccc atgtggcttt tgccaccagc gcccttctgc tctttgctga
    11641 cggagggctg ctttatgact atttcccgta tttcactccg ccaatggaca aattgggtat
    11701 tgggctgggc ttcacgctgg cggtgaaaga aagcgcattt ctgctgtgga tcttagcagc
    11761 ggtattgagt gaaaaacggc ttttacagca agtcatcgtg ctggattcgc tgggctacag
    11821 ccgctggcaa tgcctgaact ggctgctgtt gccctccgtc gcgcctgcgc tggcaatggc
    11881 gatgctggcg attgttgcct ggtcgctgtc ggtcgtagat gtggcgatta ttcttgggcc
    11941 aggtaatcca ccgacgctgg cagtaattag ctggcagtgg ttaactcagg gcgatgccga
    12001 tcaacaaacg aaaggcgcac tcgccagcct gctgctgatg ttgttactcg ccgcctacgt
    12061 tttgctgggc tatctgctat ggtgtacctg gcggcgtact attccccgcg tagatggcgt
    12121 tcgtaagcct gccacgcctt tattgccagg cattacgctg gcgagttttt tacccttgac
    12181 cagtgtgctg tgtgtggttc tgctggcgat cctcgcggat cagtcgacga tcaatagtga
    12241 agcgctcatc aacagcctga caatggggct ggtggcgaca ttcatcgctt tgctcctgtt
    12301 actgctgtgg ctggaatggg ggccacagcg tcgccagttg tggctatggt tgccgatttt
    12361 attaccggca ctgccgctgg tggcgggcca gtacacgctg gcgctatggc tgaacatgga
    12421 tggaagctgg acggcggtgg tctgggggca tctgctgtgg gtaatgccgt ggatgctgtt
    12481 tatcctgcaa cccgcctggc agcgcattga ttcacggtta attttgattg cacaaacact
    12541 gggctggtcg cgggccaaaa tcttctttta cgtgaaatgt ccgcttatgc tacgccctac
    12601 gctgattgcc ttcgcggtgg gattttcagt cagtattgcg cagtatatgc cgacgctgtg
    12661 gctgggcgcg gggcgttttc cgacgctcac cactgaggcg gtggcattaa gcagcggcgg
    12721 cagcaacggt attctcgccg ccctggcttt atggcaactg ctattaccgc ttattatttt
    12781 tgccctgacc gcgttagtcg caaaatgggt aggttatgtc agacaaggac tccgctaatg
    12841 ctctgcgtga aaaatgtttc gctacgttta ccagaaagtt gcttgctgac aaacgttaac
    12901 tttacggtgg ataaaggtga cattgtcacg ttaatggggc cgtctggctg tggaaaatcc
    12961 actctgtttt catggatgat tggtgcactg gccggacagt tttcttgtac aggtgagcta
    13021 tggctcaatg agcaacggat tgacatgcta cccaccgcac agcgtcagat tggcattctt
    13081 tttcaggatg cactgttatt tgaccagttc agtgtcgggc aaaatttact gctggcgcta
    13141 ccggcgacac ttaaggggaa tgcccgacgt aatgccgtga atgatgcact tgagcgttca
    13201 ggccttgacg gagctttcca tcaggatcct gctactttgt ctggcggtca gcgagcgcgc
    13261 gttgctctgc ttcgcgccct tctcgctcag ccaaaagcgt tgctactgga tgagccattc
    13321 agccgtcttg atgtggctct gcgcgataat tttcgccagt gggtgttcag cgaagttcgc
    13381 gaactggcga tccccgtcgt tcaggtaacg cacgatctcc aggatgttcc tgctgatagt
    13441 tctgttctgg atatggcgca gtggtcagaa aattacaaca aactgcgata acgcaaagtt
    13501 tttctcaatg cgtcagttca gaatggcgca ctcaaaacta caatgtcggg attttcgatg
    13561 aaacgtgttt ctcaaatgac cgcgctggca atggctttag ggctggcttg cgcttcttcg
    13621 tgggccgctg aactggcgaa gcctcttaca cttgaccagc ttcaacaaca aaatggcaaa
    13681 gcgatagata ctcgctccag cgcgttttat aacggctggc cacaaacctt aaatggccct
    13741 tctggtcatg aacctgccgc cttaaacctc tctgctagct ggcttgacaa aatgagcacc
    13801 gaacagctca acgcgtggat caagcaacat aacctgaaag ccgatgcccc ggtggcgctg
    13861 tacggtaatg gcaaagatgt cgacgccgtc aaaacgcgac tgcaaaaagc aggttttacg
    13921 catatctcca ttctgagtga cgcgctaagc gaaccttccc gtctacaaaa actgccgcac
    13981 tttgagcagc tggtttatcc gcaatggctg cacgacctgc aacaaggtaa agaggttacg
    14041 gcgaaacctg ccggtgactg gaaagtcatt gaagcggcca ggggcgctcc taagctttac
    14101 cttatcagcc atattcccgg tgctgactac atcgatacca acgaagtgga aagcgaaccg
    14161 ctgtggaaca aagtttctga tgaacaacta aaagcgatgc tggcaaaaca cggcattcgc
    14221 catgacacca cggtcattct gtacgggcgt gacgtatacg ctgcagcgcg tgtggcgcag
    14281 attatgcttt atgccggcgt gaaagatgtg cgcctgctgg atggcggctg gcaaacctgg
    14341 tccgacgcgg gtctgcccgt tgagcgcggt acgccaccga aagtgaaagc ggaaccggat
    14401 ttcggcgtga agatcccggc acaaccgcag ttgatgctcg atatggaaca ggcccgtgga
    14461 ctgctgcatc gccaggatgc atcgctggtg agcattcgtt cgtggccaga atttatcggt
    14521 acgaccagcg gttacagcta tattaaacca aaaggtgaaa tagccggagc acgttgggga
    14581 cacgctggta gcgactcgac gcatatggaa gatttccata atccggatgg caccatgcgt
    14641 agcgccgatg atattaccgc tatgtggaaa gcatggaata tcaaaccaga tcagcaagtt
    14701 tcattctact gcggcaccgg ctggcgcgcg tccgaaacct ttatgtacgc acgagcaatg
    14761 ggctggaata acgtctccgt ttacgacggc ggctggtacg aatggagcag cgatccaaaa
    14821 aatccggtag caaccggtga acgcggcccg gacagcagca aataacattg caattactga
    14881 cgctggagcg acttcagcgt cagataccca ctccacaccc gcgtaaaggt cgtcatccag
    14941 cacaacgcac cgaagatcca cgcaaaccac gcaaaccatg cagggaataa acatcccagc
    15001 acaaacagta agatcgtttc tgtgccttcg gttaatcccc ccagataata aaacgattta
    15061 tgcgcataac cgggattatc aatctgatat ttcgccgcca gcgcagcaaa agcgagaaaa
    15121 ctgctacccg tgccgataaa cgcaaacagc agccagccgc ctgccagggc gttttgctgc
    15181 ggtgcggcga ggataaaccc aaaaggtacc agcgcgtaaa aaagaaaatc gagagaaata
    15241 tcaagaaagc cgcccgcgtc ggtaagccct ctgcgccgcg ccagcgcacc gtccagacca
    15301 tcaagcagcc tgttcaacaa aatgacgaca agcgccgcca gataccagcc cagcgccaga
    15361 aaaggcagtg ccagcacacc gatggcaaat cccactaatg ttaaaccatc tggcgtaatg
    15421 cccggtttat caagaacccg cacacactga tgcaacaacg gtttaatccg gggatgaaga
    15481 tggcggtcta gcacaggcac tccttaaata taaagccttt ctgattgagc aacagtgcgg
    15541 atattatggc atttttcgct tatctgcccg tgtgtaattt atgaaaatga ttgaagttgt
    15601 tgccgccatc attgaacgtg atggcaaaat tttactcgcg caacgccccg cccagagcga
    15661 tcaggcggga ttatgggagt ttgccggtgg taaagtcgag ccggatgaaa gccagcagca
    15721 ggcgctggtg cgtgagttaa acgaagaatt gggcatcgaa gcaactgtgg gtgagtatgt
    15781 tgccagccat cagcgagaag tttcgggccg gattatccat cttcatgcct ggcacgtacc
    15841 cgacttccac gggacgttac aggcacatga acatcaggcg ctggtctggt gctcacctga
    15901 agaggcgctg caatatccgc tggctcctgc tgacattcca ttattagagg cgtttatggc
    15961 tttacgcgcc gccagaccag cggattagtg ctaagggttt tgtcatcccg ctggcattgc
    16021 agcagtattc cttcggcttt aattaccgcc ccttcagaat aatttttatc ctgataaacg
    16081 cagcactgag tacagggctg cgctgactgc ccgcctgaac tgaccacttc tggcggtacg
    16141 tttacctcca cgtccggacc ataatgcggg ttagccagtg caattaatgg aaatgctaat
    16201 actacggcga acaatgctcg actcacaggg aactccttaa cgttattgtc tctgctactg
    16261 ataacggtag ccgggtggca aaactttagc gtctgagtta tcgcatttgg ttatgagatt
    16321 actctcgtta ttaatttgct ttcctgggtc atttttttct tgcttaccgt cacattcttg
    16381 atggtatagt cgaaaactgc aaaagcacat gacataaaca acataagcac aatcgtatta
    16441 atatataagg gttttatatc tatggatcag acatattctc tggagtcatt cctcaaccat
    16501 gtccaaaagc gcgacccgaa tcaaaccgag ttcgcgcaag ccgttcgtga agtaatgacc
    16561 acactctggc cttttcttga acaaaatcca aaatatcgcc agatgtcatt actggagcgt
    16621 ctggttgaac cggagcgcgt gatccagttt cgcgtggtat gggttgatga tcgcaatcag
    16681 gtacaggtca accgtgcatg gcgtgtgcag ttcagctctg ccatcggccc gtacaaaggc
    16741 ggtatgcgct tccatccgtc agttaacctt tccattctta aattcctcgg cttcgaacaa
    16801 accttcaaaa atgccctgac tactctgccg atgggcggtg gtaaaggcgg cagcgatttc
    16861 gacccgaaag ggaaaagcga aggcgaagtg atgcgtttct gccaggcgct gatgactgaa
    16921 ctgtatcgcc acctgggcgc ggataccgac gttccggcag gtgatatcgg ggttggtggt
    16981 cgtgaagtcg gctttatggc ggggatgatg aaaaagctct ccaacaatac cgcctgcgtc
    17041 ttcaccggta agggcctttc atttggcggc agtcttattc gcccggaagc taccggctac
    17101 ggtctggttt atttcacaga agcaatgcta aaacgccacg gtatgggttt tgaaggcatg
    17161 cgcgtttccg tttctggctc cggcaacgtc gcccagtacg ctatcgaaaa agcgatggaa
    17221 tttggtgctc gtgtgatcac tgcgtcagac tccagcggca ctgtagttga tgaaagcgga
    17281 ttcacgaaag agaaactggc acgtcttatc gaaatcaaat ccagccgcga tggtcgagtg
    17341 gcagattacg ccaaagaatt tggtctggtc tatctcgaag gccaacagcc gtggtctgta
    17401 ccggttgata tcgccctgcc ttgcgctacc cagaacgaac tggatgttga cgccgcgcat
    17461 cagcttatcg ctaatggcgt taaagccgtc gccgaagggg caaatatgcc gaccaccatc
    17521 gaagcgactg aactgttcca gcaggcaggc gtactatttg caccgggtaa agcggctaat
    17581 gctggtggcg tcgctacatc gggcctggaa atggcacaaa acgctgcgcg cctgggctgg
    17641 aaagccgaga aagttgacgc acgtttgcat cacatcatgc tggatatcca ccatgcctgt
    17701 gttgagcatg gtggtgaagg tgagcaaacc aactacgtgc agggcgcgaa cattgccggt
    17761 tttgtgaagg ttgccgatgc gatgctggcg cagggtgtga tttaagttgt aaatgcctga
    17821 tggcgctacg cttatcaggc ctacaaatgg gcacaattca ttgcagttac gctctaatgt
    17881 aggccgggca agcgcagcgc ccccggcaaa atttcaggcg tttatgagta tttaacggat
    17941 gatgctcccc agggaacatt tcttatgggc caacggcatt tcttactgta gtgctcccaa
    18001 aactgcttgt cgtaacgata acaagcttca agttcagcat ccgttaactt tctgcgatag
    18061 cagcagatat gccagtaaag aaatcccatt agactatttt tttgataatc ttcttcgctt
    18121 tcgaacaact cgtgcgcctt tcgagaagca agcattatat aatgccaggc cagttcttct
    18181 tcaattgtcc cgttttgaaa agctgtgctt gatatcgaga tcatccatga taattccgcc
    18241 gcccatatta gcttcgccga ggatttaccg gagctatgat tagcgcaatc agagatatag
    18301 tctgagggaa aaacagcaaa tttattcaac aaggcgataa cctgctctgg ggcttcctcc
    18361 atgtttgctt taaaggtatt ggctccatgg tcgccagaaa gaaaatgctc cattaaggca
    18421 caataacttt cgctatcttc gataccccat tgttcctcta aagactcgcg tcttttactt
    18481 atgatatcga tcgagtcaaa aggaagcaca tgatattgga aggtatcttt gccaggttca
    18541 ggctttcgcg gccagaactc cagcgtttca gaccattgct tatgatagaa tcgataaggt
    18601 gcgatcaatt gtagcgcctg taatttctcg atactgagcg gctcaatacc tttagcctga
    18661 taataatgca gctgttcttt ttttgcttta aaaccggccc gaacaataag ccccatcata
    18721 attaatagat aaagaaaaga gcatcccgcg gtaatcaggc ctctttcatt caaaccgttg
    18781 gatgttatcg ctgcgaatac aaacattaca gcgacaacac atgttaaata aaacccccac
    18841 ttacaaagca gcatggtctt attttcttta atcatccgtt caaaattact attaaatatt
    18901 tcccagccat taaaagaata cttctcgctc ccaggatggt tttgtaataa aacttttttc
    18961 atcacattcc ctgttattac atactttaga ggcgataatt ttatccatgc aaaaaaaata
    19021 tccaacgaat tttcttgatc cgctggatat ttcagaatat gactcgaata gcacgaaaga
    19081 ttcgctcgct tacgctatcg ccccgcttcc ggcttcatct gctggcggac tttttttcgc
    19141 actacgttta cgcggtgcag cctttttctt atcagcactg ccaccactgc ccggagccac
    19201 aatgccgcga aactgccgca ccggagtacg tttggcttga tcaataagct gatatagcgt
    19261 ccccaccagc ggctgcataa agtcctgata gcgacactgc ttttcgctga tttgcgtcag
    19321 caccgattcc cagtgcgcgg tcatgtccgg tcgcgtagcc atttctggca gcgaatggaa
    19381 taacgctttt ccggcgtcgg tggagtggat atagcgccct tttttgatga ggaaaccacg
    19441 cttgaacaac agttcaataa tcccggcacg cgttgcttct gtccccagac catcggtcgc
    19501 acgaaggatc tttttcagat ctttgtcctg cacaaagcgc gcgatcccgg tcatcgccga
    19561 aagcagtgtt gcatcggtaa aatggcgcgg cggctgggtt tgccgctcta ccacttcacc
    19621 tttttcacac agcaactcat cgcctttcgc caccacaggc aatggcgtac cgtcgttttc
    19681 ttcatcgcgc tctttgctgc ctaacagcgt gcgccagcct gcttcagcaa gaaaacgcgc
    19741 tttagcgaca aatttgcctt tggcaatgtc cagttcgata acacacttgc ggaacaccgc
    19801 atccgggcag aactgcatca gatactgacg ggaaatcagg ttatagacct tcgcttcgtt
    19861 ctccgtcagg ttgatcgcag aactccgtgc ggtcggaatg atggcgtggt gcgcatcgac
    19921 ctttttgtca tcccaacagc ggttgcgtat atctggatct accactggct gcggcaacag
    19981 atctggtgca tgaacgctga tggcattcat caccgcgtgg cgtccggcaa aatgttcttc
    20041 tggcaaatag cgacaatcgg aacgcggata agtgattagc ttgtgcgttt cgtacagctt
    20101 ctggcagata tcaagcacgt tctgcgcgct cagaccaaag cgttttgccg cttcaatctg
    20161 caacgccgaa agcgaaaacg gcagcggcgc ggattctgat tcccgtttat cgttatagct
    20221 ggtgacaata gccggttgac cactaatgcg gttaaccaca tgctccgcca gtggacgatg
    20281 taacaagcgc ccttcttcat cctggtacgg ttcacacgct tcgctcggtt gccagatagc
    20341 ggtaaaccgc tcatcggcag gtgtcacgat atgtgctttg acttcaaaga agtctttcgc
    20401 cacgaagttt tcaatctctt catcgcggcg caccaccagc ccaagcacgg gcgtctgcac
    20461 gcgtcccacg gaaagcacgc cctgataacc ggcattgcga ccgagaatgg tatacgcacg
    20521 ggtcatattg atgccgtaca gccagtcggc acgcgctcgc gccagcgcag aaacgcacag
    20581 cggtacaaac tcactgttgg aacgaagacg gtcgatcgcc cgctcaaccg cctgcgggtt
    20641 caggtcgttt atcaagcaac gctgcacctg ctggcgcttt tccggtgcca gttgtagata
    20701 gtccagcact tcatccacca gcagttgccc ttcgcgatcc gggtccccgg cgtgaacgat
    20761 ttcgctggct tcatgcaggt accgtttgat gacgttaagt tgtttggtca cggagggtcg
    20821 gggctgtaat tgccactttt ccgggacaat cggcaaatcc gcaagattcc agcgcgcata
    20881 gcggctgtcg taggcgtctg gctgcgcctg ctcaagcagg tgaccgatac accaggtcac
    20941 cacctgacca ttaccgcact cgataaagcc atcgcctttc cggtgcggtt tgggcaggac
    21001 atcagcaatg gcgcgcgcca gactcggttt ttcggcaata aacaaccgca ttgaattaac
    21061 ggatctcaac catggcacga ccgccgcgtg ctggcaccag ttcgccaatc gccgtcagtt
    21121 caatgccaaa ctcggcggct gtagctttga cctcattttc ggcttccggc atgaccgcca
    21181 gcagcaaacc gccagaagtt tgcggatcgc acagcaaatc gcgcacttca cgcggcattt
    21241 cacccatcag atgaccgtag ctggcaaagt tacgttcggt gccgccaggc actgcaccca
    21301 acttaatgta ctcttcaacc cccggcaatt tcgggatcgc gtcatagtca acgcgcgcct
    21361 gcacacctgc gccctgacac atttcgctca aatggcccag caggccaaag cccgttacgt
    21421 cggtcatcgc ttttacgcct tcaatgttgg caaaggacgc gcctgcgatg ttcatccggc
    21481 acatcacttc cgtcgccagt ccctgatgtt ctggtttcag cagtgatttt ttctcagccg
    21541 tggtaagaac gccgatcccc agcggtttcg tcaggaacag tttacatccg gcttgtgcgg
    21601 tactgttttt cttcacccgc tcggtcggta cgatccccgt taccgccaga ccaaaaatcg
    21661 gctccggcgc atcgatggag tgaccgccag ccagcgcaat acccgcctga cggcatgcat
    21721 agcgtccacc ttcggttact tcgcgggcaa tttccgggga aagtttgtta atcggccagc
    21781 cgaggatcgc aattgccata atcggtttgc cccccatcgc gaagatatcg ctgatggcgt
    21841 tagtcgccgc aatgcggcca aaatcgaaag gattatcaac gatcggcata aagaagtcgg
    21901 tggtactgat aacgctggtg ccattgccca gatcgtacac cgccgcatcg tcgcgggttt
    21961 cattacccac aagcaaattc ggatcaacaa acttcgcctg ctcactgtgc aggatggttt
    22021 ccaacacttt tggggaaatt ttacagccgc aaccagctcc gtggctgtat tgggtcaaac
    22081 gaatcgagtt ctcgctcatg gacatctcct gtcaatgcaa tccgggtatg gtaaccctca
    22141 ttccgtgaag tgataagtga gagtgtctga attcctgcgc ctttgctcac aatccagaca
    22201 gtttcgcgac aattatcaga aataagtgac aaacggcgtc gggtccggga cgtttatcga
    22261 cgtagatgct ttcagctgcg gcgtgccgag gtagagaaaa ccgacaattt tatcctgctc
    22321 acggcaaccg aatgcttcac gcactaccgg actttcagtt aatgcgccac tgcgccagat
    22381 gccgccaaac ccctgggcaa cggctgccat ttgcatcgcc atgaccgcgc atccggcaga
    22441 catttcctgt tcccatcgcg ggactttatg attttcttcg catttcgcca ccaccgtgat
    22501 gatgagcggt gcgcggaacg gcgcattacg ggctttgtcg atagctttgt catcactacc
    22561 ggcagcaatc gccccctgtt ccagtacggc gctgaaacgc tcgcgccctt ccccttcaat
    22621 cacaaaaaaa tgccacggtt gcatggactt atggtccggc gcgcgcatac ccgcacgcag
    22681 gatgttttgc agttgttcac ccgttggtgc gggttcagcc aggcgggagg cgctacggcg
    22741 attgatcaat agttcgagtg catccatttg attaactcct gtcgtgatat ttattcacaa
    22801 aattaacacg agagtggatt ttgttacagc acagtccgca attcctgctg acaagtgccg
    22861 gttgggtcat tacgataacc acatctattg cgcctgtgac tggtgtgacc ttaagttggg
    22921 agaatacatg cgaacccttt ggcgatttat tgccggattt tttaaatgga cgtggcgtct
    22981 gctgaatttc gtccgtgaaa tggtacttaa cctgttcttt atttttctcg tgctggttgg
    23041 tgtggggatc tggatgcagg tcagtggtgg tgattcgaaa gaaacggcca gtcgtggtgc
    23101 actgctgctg gacatttctg gtgtgatcgt cgataaaccc gacagttctc agcggtttag
    23161 taaattaagc cgccaactac ttggtgccag ttccgatcgt ctgcaggaaa actcactgtt
    23221 tgatatcgtc aataccattc gccaggcgaa ggatgaccgc aatatcaccg gtatcgtgat
    23281 ggatctgaaa aacttcgcag gcggcgacca accgtctatg cagtacatcg gcaaagccct
    23341 gaaagagttt cgtgacagcg ggaaaccggt ttatgccgtt ggcgagaact acagccaggg
    23401 gcaatattat ctcgccagtt tcgccaataa aatttggctg tcaccgcaag gcgtggtgga
    23461 tttgcacggt tttgctacca acggtctgta ctacaaatcg ttgctggata agctgaaagt
    23521 ttccacccat gtgttccgcg tgggtacgta taaatctgcc gttgaaccgt ttattcgtga
    23581 tgacatgtca ccggcagccc gcgaagctga cagccgctgg attggtgagc tgtggcaaaa
    23641 ctatctgaat actgttgccg ctaaccggca gatccctgct cagcaggtat tccctggcgc
    23701 gcaagggttg cttgagggtt taaccaaaac cggtggcgat accgcgaaat atgcactgga
    23761 aaacaagctg gtcgatgcac tggcatcgag tgcggaaatc gaaaaagcac tgaccaaaga
    23821 gttcggctgg agtaaggctg ataaaaatta tcgcgccatc agttattacg attacgcatt
    23881 gaaaacgccg gcagataccg gtgacagcat cggtgtcgtc ttcgctaatg gcgcaattat
    23941 ggatggcgag gaaactcagg ggaatgttgg cggtgatacc actgcggcac aaatccgcga
    24001 cgctcgcctt gacccgaaag tgaaagcgat tgtcctgcgt gttaatagcc caggcggcag
    24061 cgttaccgcg tctgaagtga ttcgcgctga actggcagca gcccgggcag cgggtaagcc
    24121 tgtggttgta tcgatgggcg gcatggcggc atctggtggt tactggattt ccacgccagc
    24181 taattacatt gtggctaacc ccagcaccct gaccggttct attggtatct tcggcgtgat
    24241 caccaccgta gaaaatagtc tggattcgat tggtgttcat actgatggtg tctcaacttc
    24301 accgctggcg gatgtttcta tcaccagggc actgccgccg gaagcgcagc agatgatgca
    24361 gttaagcatt gagaatggct ataaacgctt tatcacgctg gttgctgatg cgcgtcattc
    24421 gacgccggag cagattgata aaatcgccca gggccacgtc tggaccggtc aggacgcaaa
    24481 agctaacggg ctggtcgata gtctcgggga tttcgatgat gcggtcgcca aagcagcaga
    24541 gctggcaaaa gcgaaacagt ggcatctgga atactacgtt gatgaaccga ccttcttcga
    24601 caaagtgatg gacaacatgt ctggttctgt ccgggcaatg ttgccagatg cgttccaggc
    24661 catgttacct gcaccgctgg cctcggtagc ctctaccgtt aaaagtgaaa gcgacaagct
    24721 ggcggcgttt aacgacccac aaaaccgtta tgcgttttgc ctgacctgcg ccaacgtgcg
    24781 ttaaatcctg caaaaatgtg gccgatatgc tgtcggccac actcctgaat cggactgtct
    24841 ttcactccct ctctccacta cattcccttg ctcatttagc atctgatatg gagtcatcag
    24901 tgaacaaggc cccctcttta attgccgcta tcgtcctcgg actggggatt agcgcctgtg
    24961 gttattttgt tggcgatggt gttaaacatt taaaaaccaa caaccgttat gtcaatgtgc
    25021 gcgggctttc tgaaaaagaa gttcgcgcag atacggcgga attaacaatt gcgattaact
    25081 ttaagggtaa tgttcccggc gaactgttcc cgaaactgga agaagcgcag aaaaaaattg
    25141 ttgccgaact caatgctcag gggattaacg agaaagagat aattctgggc caatggacaa
    25201 gcaagcgaac agattccttt tatctgaaag atgatcccac aatgccacgc tataacgcag
    25261 acgggtcagt gacgataaaa acccataatg ttgctgctgt cgaaaaagtc gtcgcgaaat
    25321 taaacgaact gcaagtcgct acggatggcg ctatcgctga gagcaaagtc gcctatcgct
    25381 ttaatggtat tggcgcactg cgcgctgaga tgattgctgc cgccaccaaa gatgcacgca
    25441 acgccgcact acaatttgca acggactcag gtagccaggt ggggtcaatt agcgatgcct
    25501 cacaaggcgt attccagatc tttgccagtg gcagtgatga agatgatcct acggcgatta
    25561 ataaaacggt acgcgtagtc acgaccgtaa cctatgcgtt gcaggattaa ccacttttct
    25621 ccggcgggac actaatccct ccgtcctgcc ggacttttct catatccaca gagtatccag
    25681 gtgcgctcgc tgcccctcac gtatatactt ttgctctttc gatatcattc atatcaatat
    25741 catgcaaaag aaatcaattt acgttgccta cacgggcggg accatcggga tgcagcgttc
    25801 cgagcagggt tatataccgg tgtcaggtca tctacaacgc caactggcgc tgatgccgga
    25861 attccatcgc ccggagatgc cagatttcac cattcatgaa tatacgccgc tgatggattc
    25921 ttcagatatg acgccggaag actggcagca tattgctgaa gatattaaag cgcactatga
    25981 cgactatgat ggttttgtca ttctgcacgg caccgacacg atggcgtata ccgcctctgc
    26041 gctgtcgttc atgctcgaaa atctcggtaa accggtcatt gtgacagggt cacaaatccc
    26101 gctggctgag ttacgctctg acggacaaat taatctgctg aatgcgttgt acgttgcggc
    26161 gaattatccg atcaacgaag taacactctt ttttaataac cgactgtatc gcggcaaccg
    26221 cactaccaaa gcccatgccg atggttttga tgcgtttgcc tctccaaacc ttcctccgtt
    26281 actggaagca ggtatccata ttcgtcgttt gaatacgcca cccgccccgc acggtgaagg
    26341 ggaattgatc gtacatccaa tcaccccaca accaattggc gtagtgacga tttatccagg
    26401 gatttctgct gacgtcgtgc gcaattttct gcgccaaccg gtgaaagcat tgattctgcg
    26461 ctcctatggc gtgggtaatg cgccacaaaa caaagccttc ctgcaggaat tacaagaagc
    26521 cagcgatcgc ggtattgtgg tggtcaacct gacacaatgt atgtccggta aagtgaatat
    26581 gggtggttat gccaccggta acgccctcgc ccatgctggc gttattggcg gtgcagatat
    26641 gactgtagaa gccacgctaa ccaaactgca ttacctgctt agccaggaac tggatactga
    26701 aaccattcgc aaggccatga gccaaaacct gcgcggcgaa ctgacgccgg atgattaagg
    26761 agactgcaat gccccctcgc gccctgttac tggtcgattt acaaaatgat ttctgtgctg
    26821 gtggcgcgct cgccgtgccg gaaggtgaca gtacggtgga tgtcgctaac cgcctgattg
    26881 actggtgcca gtcgcgcggt gaagcggtta tcgccagtca ggactggcac ccggcgaatc
    26941 acggcagttt tgccagccag cacggtgtag agccttatac gccaggccaa ctcgacggtt
    27001 tgccacaaac cttctggcca gatcactgtg tgcagaacag tgaaggcgca caattacatc
    27061 cgttactgaa ccaaaaagcg atcgcagcgg tgttccataa aggcgaaaat cctttagttg
    27121 acagttacag tgcctttttt gataacggcc gtcggcagaa aacctctctc gatgactggt
    27181 tacgcgatca tgaaatcgat gaattgatcg ttatgggcct ggctactgac tattgcgtga
    27241 agtttaccgt gctggacgcg ttacagttag gttataaggt aaacgtgatt accgatggtt
    27301 gtcgtggcgt gaatatccag ccccaggaca gtgcgcacgc gtttatggag atgtcagcag
    27361 ctggggcaac gctatatacg ctggcagact gggaagagac gcaggggtaa ttttacgctg
    27421 gcctacaatt ctgtactggc attgtaggcc aaataaaaca cgtcagtggc acatctggca
    27481 attgatgcca tcaacgaaag atttaattta cctcagaaat ctcttccaac gacaccttcc
    27541 gcgtttcgat gccaaaaatg gagagaacca gcgcacagag caataacatg acaccaagta
    27601 ccataaacac cgtgatcgac ccataatgtg ttaataatgc cgcaacgcca tagggcgtga
    27661 agactgcgac gatccgtccg acggcattaa cgaaacccga accgcgcagg cgtaaatgcg
    27721 ttggccaaag ctccgggata taaaccgccg acgcgaagca aacatacatg tataaaaaga
    27781 agatcatcac caggccatag attaaaatcg cccactctgt agtctgaatt gaatagatat
    27841 agcctaacac tgcaataata ataagtaagg tggagccaaa taaccgacga ggaaaatgat
    27901 caataattaa tgccgcaata aatattccta ccggagcgcc aatcataata acagcggtca
    27961 ttaatattga tttatcgaca tcaatgccgg agttaacaaa tatggtcggt atccatacgg
    28021 tgatggtata aagcgaaatg ttcattgcaa ttaaaacagt aatcgcgact aatgtacgtc
    28081 gtaacatttc acctttaaac aggagccaga aagtaccctt gattacttta accttgctgt
    28141 tgctctgata cgaagtaagc gggggtaaac gaatactctt ctctctttca atttgctgct
    28201 ctacttcacg aagttggcat tctgcacctg cgatttgccc tttccctgcc agccatcgtg
    28261 gcgactcaat aaagtattta cctgagagaa accaggctaa cagtatgcca ataccaccca
    28321 acagaaacat tatccgccaa ctaaaaaaag cgataaccac cacacctatc gccgcagaca
    28381 gcatgggcga ccagttacca acaaatgaga gccgcgcgga ccattttcca cgcaccgtcg
    28441 cggggataaa ctccgtaaat gaggcatagc caaccataat cagcgccccc attcctgttc
    28501 ccatcaggaa gcgaaagaag ataagccagt acatatcagg gacaaaagcg gcccctgttg
    28561 cagcaatacc gacgatgaga agatttatgc gaaacgccct gcgccgccca aagtaatcac
    28621 cgatgaaccc accggtaaga gagccgatga aataaccaaa cattaatgcc gaggtaaagg
    28681 cggcattgag gaaattattt gaccatccat tgcttaccag ctttgctaag acgacattac
    28741 cggagtaact caaaaaccct
//
LOCUS       DAZKIF010000056        28265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZKIF010000056 DAZKIF010000000
VERSION     DAZKIF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.056"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(107..1504)
                     /gene="ccp"
                     /locus_tag="ACHOTW_002386"
     CDS             complement(107..1504)
                     /gene="ccp"
                     /locus_tag="ACHOTW_002386"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP9844486.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1909..3558
                     /locus_tag="ACHOTW_002387"
     CDS             1909..3558
                     /locus_tag="ACHOTW_002387"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP9844487.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3609..4211)
                     /locus_tag="ACHOTW_002388"
     CDS             complement(3609..4211)
                     /locus_tag="ACHOTW_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9844488.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4731..5630
                     /locus_tag="ACHOTW_002389"
     CDS             4731..5630
                     /locus_tag="ACHOTW_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9844489.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5679..6692
                     /gene="yhjD"
                     /locus_tag="ACHOTW_002390"
     CDS             5679..6692
                     /gene="yhjD"
                     /locus_tag="ACHOTW_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP9844490.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7104..8426
                     /locus_tag="ACHOTW_002391"
     CDS             7104..8426
                     /locus_tag="ACHOTW_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9844491.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8608..10668)
                     /locus_tag="ACHOTW_002392"
     CDS             complement(8608..10668)
                     /locus_tag="ACHOTW_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9844492.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10738..11505)
                     /gene="pdeH"
                     /locus_tag="ACHOTW_002393"
     CDS             complement(10738..11505)
                     /gene="pdeH"
                     /locus_tag="ACHOTW_002393"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP9844493.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11737..12666
                     /gene="kdgK"
                     /locus_tag="ACHOTW_002394"
     CDS             11737..12666
                     /gene="kdgK"
                     /locus_tag="ACHOTW_002394"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP9844494.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12762..14258)
                     /locus_tag="ACHOTW_002395"
     CDS             complement(12762..14258)
                     /locus_tag="ACHOTW_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9844495.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14479..15765)
                     /gene="dctA"
                     /locus_tag="ACHOTW_002396"
     CDS             complement(14479..15765)
                     /gene="dctA"
                     /locus_tag="ACHOTW_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP9844496.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            complement(15948..17936)
                     /gene="hmsP"
                     /locus_tag="ACHOTW_002397"
     CDS             complement(15948..17936)
                     /gene="hmsP"
                     /locus_tag="ACHOTW_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP9844497.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18018..21481)
                     /gene="bcsC"
                     /locus_tag="ACHOTW_002398"
                     /pseudo
     CDS             complement(18018..21481)
                     /gene="bcsC"
                     /locus_tag="ACHOTW_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            complement(21463..22569)
                     /gene="bcsZ"
                     /locus_tag="ACHOTW_002399"
     CDS             complement(21463..22569)
                     /gene="bcsZ"
                     /locus_tag="ACHOTW_002399"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP9844498.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22576..24942)
                     /gene="bcsB"
                     /locus_tag="ACHOTW_002400"
     CDS             complement(22576..24942)
                     /gene="bcsB"
                     /locus_tag="ACHOTW_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFP9844499.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24953..27571)
                     /gene="bcsA"
                     /locus_tag="ACHOTW_002401"
     CDS             complement(24953..27571)
                     /gene="bcsA"
                     /locus_tag="ACHOTW_002401"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP9844500.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27568..28265)
                     /gene="bcsQ"
                     /locus_tag="ACHOTW_002402"
                     /pseudo
     CDS             complement(27568..28265)
                     /gene="bcsQ"
                     /locus_tag="ACHOTW_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
BASE COUNT         6674 a         7832 c         7115 g         6644 t
ORIGIN      
        1 aaaatgatct tttataatat aaacatttaa tatgataata ttaaaaacag actttattat
       61 cgtaataaaa atcgcccaaa cattcctgtt cgggcgatca aattaattat tgtttatcct
      121 gcatatacgg cgtgtaaacc ccgttcagac tgtgcaggaa agctacgata tcatccacat
      181 cctcctgcgg cagctctttg cctacctgat agcgcaacat cagtttcacc gccccgtcca
      241 gcgtcggcac gtcaccgcga tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg
      301 gtactttctg acgcaattta tcacgctctt ctttagtcac attcatacga ccaatatccg
      361 ccgccgtaat ttccccaaag ttaaagtctt ttttcagccc caacggttcg aaggaacgtc
      421 cgccgagaat aattccacca tgacaagttg cacatttatt atctttaaat aattgatagc
      481 cttttttctg ttgcgccgtc agcgcatttt catccccacg caaccattta tcaaatgggg
      541 aatccggcgt aattaatgtt ttctcaaatt cagcaatggc atcagtaata ttttcgccac
      601 tgaaaccttg cggatagacg tcgaggaact gcgctttaag ctgcggatct ttttccagct
      661 tagcaataat ttcgtcccag gattttgacg ccatttcaat cgggttcaac ggcggtccac
      721 cagcctgatc ctgcaatgtt gccgcacgac catcccagaa ctgctcaacg ttaaatactg
      781 agttaaatac cgtcggcgcg ttaatcggcc caactgcgcc accaacacca atcgatgttt
      841 ttctgccatc gacgcccccc gcattcaacg catggcaatg agcgcatgaa atggtgctat
      901 cagccgataa acggggatcg tgatacagcg caaagcccaa cgccactttt tgcgcatcgg
      961 taggcagttt ttgcgggatg ggctgcaccg gttcattgcg atgctccgga gcagtatcat
     1021 tgctggcgta atattccgcg cgctgttttg caatccaggc cagtatttcc gcccgctctt
     1081 catcactcac cttacccgcc cagtgtagcg cggtataacg cgttggcggc atagtttcat
     1141 actgcatcac ccattcaatc ttattcagat cgctttgcga aacgggttta tcagccagca
     1201 gagcagcacg cacggcctcg aggttaaaag atttatatcc aagcttaatg tcgtaatcca
     1261 tcaactgttt cgcgccagga atataataat aggcgggtaa ttctgccgaa ggcgtgtggc
     1321 aatagtcgca tcctttttcg cggagaaagc ctaaaacctt attattttca ctgacagcag
     1381 atgcctgaac atcggcctgt ttactgcgtt tattatcgtg gtaccacaca taaccagata
     1441 accctaaata gcaaatcgcg acgccagcca ggccgatcgc ggtaatacgt gagaccattt
     1501 tcattattct tccctcacgg tggtgagtta ttgtgaccca gaaaattttc cagaatgctg
     1561 caaacgtctc tgcgagacaa agccatcata aaaaacaggg agatacgctt tttgatagga
     1621 atcaatcaat tacatgaata taaactatca ataagatagc ctgtgcctat tacccagatc
     1681 aggaatggca tatcatgatt gcggcaagag caggattgaa tgttctgaaa atgaaaaaca
     1741 acacgaaatc tgtattttta taaattccat tgatattagt gcgttatatt ttttgacagg
     1801 ttaataaacg tgatccaccg cacgctttgt cgcccaccag gcggagcgaa tgactaccct
     1861 taaagaaaag cccgataatt agcgacgaat ttcggaggtt ggatccttat gctcaatcag
     1921 aaaattcaaa accctaatcc agacgaactg atgatcgagg tcgatctctg ctatgaactg
     1981 gacccgtatg aattaaaact ggatgagatg atcgaggcag aaccggaacc cgagatgatt
     2041 gaagggctgc ccgcctctga tgcgctgacg cctgccgatc gctatctcga actgttcgag
     2101 catgttcagt cggcgaaaat tttccccgac agtaaaacct ttcccgactg cgcacccaaa
     2161 atggacccgc tggatatttt aatccgctac cgtaaagtgc gccgtcatcg tgattttgac
     2221 ttgcgcaagt ttgttgaaaa tcacttctgg ctgccggagg tctactccag cgagtatgta
     2281 tcggacccgc aaaattccct gaaagagcat atcgaccagc tgtggccggt gctaacccgc
     2341 gaaccacagg atcacattcc gtggtcttct ctgctggcgc tgccgcagtc atatattgtc
     2401 ccgggcggcc gttttagcga aacctactat tgggattcct atttcaccat gctggggctg
     2461 gcggaaagtg gtcgggaaga tttgctgaaa tgcatggccg ataacttcgc ctggatgatc
     2521 gaaaactacg gtcacatccc caacggcaac cgcacctatt atttgagccg ctcgcaacca
     2581 ccggtttttg cgctgatggt ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc
     2641 tatctcgacc acctgaaaat ggaatatgcc ttctggatgg acggtgcaga atcgttgatc
     2701 cctaatcagg cctatcgcca tgttgtgcgg atgccggacg gatcgctgct caaccgttac
     2761 tgggacgatc gcgacacgcc gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa
     2821 cattctggtc gcccgcccaa cgaggtgtac cgcgatttac gcgcgggggc ggcctccggt
     2881 tgggattact cttcccgttg gctccgtgat actggtcgtc tggcgagcat tcgtaccacc
     2941 cagttcatcc ccatcgatct gaatgccttc ctgtttaaac tggagagcgc catcgccaac
     3001 atctcggcgc tgaaaggtga gaaagagaca gaagcactgt tccgccagaa ggccagtgcc
     3061 cgtcgcgatg cggtaaaccg ttacctctgg gatgatgaaa acggcatcta ccgcgattac
     3121 gactggcgac gcgaacaact ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc
     3181 ggtatggcga accatgaaca ggccgatcgt ctggcaaacg ccgtgcgcag tcggttactg
     3241 acacctggcg ggattctggc aagcgagtac gaaaccggtg aacagtggga taaacccaac
     3301 ggctgggcac cgttacaatg gatggcaatt cagggattta aaatgtatgg cgatgacctt
     3361 ctgggcgatg aaatcgcgcg cagctggctg aagacggtga atcagtttta tctggaacag
     3421 cacaaactga tcgaaaaata ccatattgcc gatggtgttc cccgcgaagg cggcggtggc
     3481 gagtatccgt tgcaggatgg gtttggctgg actaacggtg tggtacgccg tttaattggt
     3541 ttgtacggcg aaccataata tttttacagc cagccgctaa cttcctgctg gctgtaaaat
     3601 tatcctcttc aggaggagat atttaacatc attgccgcct gggtgcgatt tttcacttcc
     3661 agacggcgat acagggattc cagatgcgct tttaccgttc cggtactgat attcagcgct
     3721 ctgccgatct ccttatttga ttcgcccgcc gctaacatgg ttaaaatctc ccgctggcgg
     3781 gcgcttaacg atttgagatc tttaatgtcc ttttccggcg tcgtccgcca gtctccaggc
     3841 agaaacatta tccccatcgc cgcactattt accgccaacg caaatgtctc gacggttgaa
     3901 tcacgaggca caatggccag cacattaaaa tggataactt cctgtaacca ccgtttattg
     3961 cagtccgtcg ccgtaattaa caccttaacc tcaggaaatt gcaccacggt tttttgcagc
     4021 aaccagtagc aaaactcacc atcctgatcg ccatcgagca taactaaggc ttcagggtaa
     4081 ctttccagct tttgccataa ctcgtctgcc tgactggccc cctgaatact cactcctgga
     4141 atacgctgct gtaaactgat tttcattcca tgaataaata ttgactgcct gtcaaacatg
     4201 actatttgca taactgaatc tccacctgaa tacgttaaaa agacttaagt agtggaaggg
     4261 tattacccgc gagaaaaaat aagaattcgc catttggcgg tggccattct acagagatgg
     4321 cgcgtagaaa atagttaccg atataaatag ttacagctaa acgcctgaaa ttacatgtcg
     4381 agggcactat ttaaaacaat tttgaggatt tccttatatt ggtggttagt acgcatgcaa
     4441 ttaaaaatga aattccgcga ccacaagcca aaataacaaa tggcaatgag acaaaaataa
     4501 gcacaaatag ccaatatgtc ctctattcac tttaaaggga atcgctgaaa aatacgctct
     4561 gtttaagggg attcaccttt ctcagaaagc tattccgccc ttttcctgct gagaaatcgc
     4621 cacattcgtc atgacaacat tgtgaaaccc ggcattagat gttagaaaaa ataataaaca
     4681 atgcgatatg cgcgttactg tttcgctgtg aacaacaatc ggtcaaagaa atggataaaa
     4741 ttcacgcaat gcagttgttc atcaaagtcg cggagctgga aagtttttcc cgcgcagcgg
     4801 atttctttgc tttgccaaaa ggaagtgttt cgcgccagat acaggcactg gaacatcaac
     4861 ttggcaccca gcttctccag cgcaccacgc gacgggtcaa actcacgcca gaaggcatga
     4921 cctattatca acgagcaaaa gatgtgttga gtaatctcaa cgaactggac ggtctgtttc
     4981 agcaggatgc caccagtatc agcggtaaat tacgcatcga catcccgcca ggaatcgcaa
     5041 aaagcctgtt actgccgcac ctgtcggaat ttctctatca gcatccggga attgagctgg
     5101 aactgagtag ccatgaccgt ccggtagata ttcttcatga tggttttgat tgcgtgatac
     5161 gcactggcgc gttaccggaa gatggcgtta tcgcccgtcc cctcggcaaa ctgaccatgg
     5221 tcaactgtgc cagtccgcac tatctgacgc gctttggtta tccgcaaaac cccgacgatc
     5281 tcacttcaca cgcaatagtg cgttatacac cgcacctggg tgtacatccg ttaggttttg
     5341 aggttgccag cgttaatggc gtccagtggt ttaagtctgg cggcatgttg acggtaaaca
     5401 gtagcgaaaa ctatctcgcc gccggtcttg ccggtctggg gattattcag atcccgcgca
     5461 ttgccgtgcg cgaagccctg cgtgccgggc ggcttattga agtgttgcca ggctaccgtg
     5521 ccgagccgct ctccctttcg ctggtttatc cgcagcgtcg ggagctttcc cggcgtgtaa
     5581 acctgtttat gcagtggctg gctggcgtaa tgaaagagta cctggactga ccgactatac
     5641 tttttaagaa tgaccacgac agaaggacaa aagagcggat gacgcaggaa aacgagatca
     5701 aacgtcccac ccaggatctg gagcacgagc cgattaagca gttggataat agcgagaaag
     5761 gcggcaaagt tagccaggcg ctggaaaccg tcaccaccac tgccgaaaaa gtccaacgcc
     5821 aaccggttat tgcgcacctg attcgcgcga cagaacgctt taacgatcgg ctgggtaacc
     5881 agtttggtgc ggctatcacc tatttctcat ttttgtcgat gatcccgatt ttgatggtgt
     5941 cgtttgccgc agggggcttt gtgctggcct cccatccgat gttgctacag gatatcttcg
     6001 acaaaattct gcaaaacatc agcgatccga cgctagccgc tacgttgaaa aacaccatca
     6061 acaccgccgt tcagcagcgt acgactgtag ggcttgtcgg cctggcggtg gcgctttatt
     6121 ccggcatcaa ctggatgggt aacctgcgtg aagcgattcg tgcccagtcg cgcgatgtct
     6181 gggaacgctc gccgcaagat caggagaagt tctgggtaaa atatctgcgt gattttattt
     6241 cgctgattgg tttgttgatt gcgctgattg tgacgctttc gatcacctcg gttgccggtt
     6301 cggcgcagca aatgattatt agcgccctgc acctgaacag cattgagtgg ctgaaaccga
     6361 cgtggcggtt gattggcctg gcgatttcca tcttcgccaa ctatctgctt ttcttctgga
     6421 tcttctggcg gttgccgcgc caccgtccgc gcaaaaaagc cctgattcgc ggaacatttc
     6481 tggctgctat tggttttgaa gtgattaaaa tcgtgatgac ctacaccctg ccatcgttga
     6541 tgaaatcccc ctctggcgca gcatttggtt ccgtgctggg gctgatggca tttttctact
     6601 tcttcgcccg tttgacgctg ttttgcgcgg cgtggattgc caccgccgaa tataaagacg
     6661 acccgcgaat gccggggaaa acgcagcctt aaaataggcc ggatgcagtt caaaacgcct
     6721 tatccggcat acgaatttat tcggcctggc tccccgtagg ccgaataaga tgcgccagca
     6781 tcgcatccgg ctataatgcg cacataacct cttgaaactc tttcccagag cctcttcagc
     6841 catctattca ggagcaaaca atttcatgcc aactcataac cccagcatat aaatccagtt
     6901 ggtaactttt atttaacctg aaaccagttt tatccactat ttataaaatt atgtgaagca
     6961 tttcatagaa gaaaaatcac tggcctaaac attatcccct ttttgcctgg tttttgacca
     7021 tttccgcgat ttgttacaca ttgaaatatc acttttgctg tgcgtaatat ggctattcgt
     7081 tagccaaaaa ataagaaaag attatgcaag caacagccac aacactcgac cacgagcaag
     7141 aatacacgcc gatcaactcg cgtaataaag tccttgtcgc ctctctcatt ggcacggcca
     7201 ttgagttctt cgacttttac atttacgcca ctgcggccgt tattgtgttt ccgcatatct
     7261 tcttcccgca gggcgatcct acggcagcaa cgctacagtc gctcgccacc ttcgccatcg
     7321 ccttcgtcgc gcgccccatt ggctctgccg tttttggtca ttttggcgat cgcgttgggc
     7381 gtaaagcgac gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc
     7441 tgctgccagg ctatgccacg attggtattt tcgccccgct gctgctggcg ctggctcgat
     7501 ttggtcaggg tctgggctta ggtggtgaat ggggcggcgc ggcgctgctg gcgactgaaa
     7561 acgccccacc gcgcaaacgt gcattgtacg gctccttccc gcagctgggc gcaccgattg
     7621 gcttcttctt tgccaacggc actttcttgc tgctttcctg gctactgacc gacgagcagt
     7681 ttatgagctg gggctggcgt gtgccattta tcttctcggc ggtgctggtc attatcggcc
     7741 tgtatgttcg cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa
     7801 aacaggtgaa gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg
     7861 gtacgttcat tatgctggca acctatacgc tgttttacat catgacggtc tactctatga
     7921 cctttagtac cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga
     7981 tgttgatgat ggcagttatt ggttttggcg tgatggtgcc agtcgctgga ttactggctg
     8041 atgcctttgg tcgccgtaaa agcatggtaa tcatcaccac gctgatcatc ctgttcgcgc
     8101 tgttcgcctt taacccactg ctcggttctg gcaacccgat tctggttttt gccttcctgc
     8161 tgctggggtt aagtctgatg ggtctgacct tcgggccaat gggtgcgctg ttaccagagc
     8221 tgtttccgac agaagtgcgt tacaccggag catcgttctc ttacaacgta gcgtcgattc
     8281 tcggggcttc cgttgcgcca tatatcgcag cctggttgca gactaactac gggctaagtg
     8341 cggtggggtt atatctggcg gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc
     8401 atgagacgcg acatcagtcg ttgtaataag agtgaaaagc ctgatgcgct acgcttatca
     8461 ggcctgaatt tccatacaat atattgaatt cgcaaggatt tgtaggccgg ataaggcgtt
     8521 cacgccgcat ccggcatgaa caaagcacac tttgccaaca atctaaatct gtttaattgc
     8581 cggatgtcag acatccggca attatcttca cttcttcatc tgcgacaaaa tcgtccggca
     8641 ctgattagcc tccccttcgg aaggtgagat cagtgccagc aacgctgccg ctggcgtaac
     8701 cagcgttgcc agtgccgcag caacagcacc acgggcaatc aacggcccgg ctttcacccc
     8761 agcctgagga tttttaaacg tcccacgcac atacagcggc gaacgcagtg tgataatccg
     8821 aataccttta ctctccggat caatagtcaa atccagctgt tccgaagcaa aacttgccgt
     8881 gccggtaacg ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg ggcgtgccac
     8941 gccgttggca atattcagat tcgccgccgc gcagttcacc cgcacctcat cgtcaccaaa
     9001 tatcgcaccg acaatgtagt tgccgacatt cagcccaaca atctccatca ggttgcggct
     9061 caccagcccg tcattcatca acagtttcag gttgccgtta ctgttgccta aaagtgccgc
     9121 caccgagtta ccgctaccgc gcagttccgc gtcaccgttc atttccccca gcgtcttctg
     9181 catcagttcc acatcgggca tcagttcttt cagtttcagt cgacgagcct gaatatctgc
     9241 ccgcccctgc atcggctttt tatcgccttc cagatgaata ttcgccgcaa tgctgccacc
     9301 cgcaatgcca aatttcagcg gttgcaggcg caagtcagca tttttgagga tgatatgagt
     9361 agaaagatcg ctaatcggca ggctactgcc atgctcaatg cgccgccctt tgaagcgaac
     9421 atcggcatcc ataacgtccc atttatcggt ttcgaagcgg tcataaggca gcactttgcc
     9481 cgcaggctga acgctttttt cgcccttctt ctgttcagac cgtttcgact tttctgcccc
     9541 tttcccggaa tcaacgccaa tcaacggtcc caggtccgcc agccgcaatt gccgcgactc
     9601 gacatcacct tccagttttg gtcgtggctt gccggtggtg tagaccagag aaccgtggat
     9661 atcgctatcg ccaattcgcc cattaaagcc gcgataatca aagaccgacg atttttcagt
     9721 gtcgattttc gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac
     9781 gcccgtcagt tcatagagat cacccagtga atcgccagaa aatttaagcc gtaaatcgac
     9841 accgcccatc ttcattgggt cattcacgac gccatcaaaa gcaacacggg tattacctga
     9901 acggaaatca gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc
     9961 tatttttccc gtacccgtga gcggttcacc gttataacgt ccctgcgcct tcaggccaaa
    10021 aacgtaatcg cccacctttt ctttatccgc tttacctttc gatccagtaa cttcgctgaa
    10081 cggcagcggc ttgcctaagg gatcaacaaa aatctccaga tccgctttgc ttactttgtc
    10141 atcaatggcg atccgccctt gatcgaaaag aatattatcc agccgaaacg accatgccga
    10201 cggctttgca ttcgcgtctt tgttatcatc gttggcgaga ttaaacgtcc agttattgtt
    10261 cttttcagag aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca
    10321 gaccgttttg gtcagcagcg ccagcggggc cagcgttgct tctacgcgtg gcaaatgcac
    10381 catcgtgact tcgggaatat ccggtggatt gccaagaatg atgtcttccg cgtgtacatg
    10441 gggccacggc acccagctgc gccagccagt ttcttgcttt tgccgctccc acaccacgcc
    10501 cagatcgcca cggatagcga acggacgatt caactccgca gagactttct ggttgatggt
    10561 cggtttgagt cgattccagt caaaagttgc aatcaaaatg atcgccacga caatcaacaa
    10621 caagaaagcc cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct
    10681 catattcttc ctgtgccagt cctaaagata gtccagccag gcggaaaatg aggcagctta
    10741 tagcgccaga accgccgtat tcagcgtttc tatcggtgcc gggcgtgaaa gaaaccagcc
    10801 ttgtgcggcg aatgcgggcg agttctgtac atcacgccac tcttccggcg tttctacgcc
    10861 ctcgacaatc accccgcgac aatagcgatt cattagatgt aaaagctgag aaaagagtgt
    10921 gcgtccttcc ggcgactgac gcagcatcac aaacagttct cgcgcgattt tgatgtagtc
    10981 ataacgcact tcacttagtg cagagaaatt tgccatcccg gtaccaaaat catccagcca
    11041 cagcgggcca aattcacaca tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc
    11101 caccagttcg aaacgcagcc agggaagacg ctcaatctgg cgcaggattt ttggttgctg
    11161 acgcagggcg atgagcgtag gaccatcaat attgaccgat gccagcaggc cgtgctctat
    11221 aaagaagtcg gctttttgcg ccagcaaatc aatctgctct ttcacaacct ccatccgatg
    11281 gctgacggtg atttcagtaa aatagcgatc cggcggcagg cgttgcgaag ggttcaaggg
    11341 atgcgtgacc gccgttaata gctccacggc cattaaccgc ccgcatgttt gatagatcgg
    11401 ctgccaggtg taagcacgct cacactgcaa ccaaaaacgc cgttcctgca agctctcgat
    11461 gcttgcttca gggttgctta ttcgctggat aacctgcctt atcatcaaag atgtcctgtg
    11521 tttaaaagtg atgcagcccg gactcgacaa agattatcgg cgcgcaaggg cagaacttta
    11581 ccgcctggcc ggggtaaatt tccccactcc acaacggaat ctttcaggaa tacgttctgg
    11641 ctcaaaaaaa taatggaaca ctgttttaat atggttgacc agcaaaccac cacagcgcaa
    11701 actaacgcta attttttaca gatcaggttc acgactatgt ccaaaaagat tgccgtgatt
    11761 ggcgaatgca tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc
    11821 gataccctga acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt
    11881 cattacgtaa cggcgctggg aacggacagt tttagccagc agatgctgga cgcctggcac
    11941 ggcgagaacg ttgatacttc cctgacccaa cggatggaaa accgtctgcc gggcctttac
    12001 tacattgaaa ccgacagcac cggcgaacgt acgttctact actggcgtaa cgaagccgcc
    12061 gccaaattct ggctggagag cgatcaatcc gcggcgattt gcgaagagct ggcgaatttc
    12121 gattatctct acctgagcgg gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag
    12181 ctgctttccc tgctgcgcga atgccgcgcc aacggcggaa aagtgatttt cgacaataac
    12241 tatcgtccgc gcctgtgggc cagcaaagaa gagacacagc aggtgtacca acaaatgctg
    12301 gaatgcacgg atatcgcctt cctgacgctg gacgatgaag acgcgctgtg gggtcaacag
    12361 ccggtggaag acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa
    12421 cgcggggcgg attcttgcct ggtgtccatt gctggcgaag ggttagtgga tgttccggcg
    12481 gtgaaactgc cgaaagaaaa agtgatcgat accaccgcag ctggcgactc tttcagtgcc
    12541 ggttatctgg cggtacgttt gacgggcggc agtgcggaaa acgcagcgaa acgcgggcac
    12601 ctgaccgcaa gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca
    12661 gcgtaaacgc agcatatgca aaaggctgga taaagagtta cccctctatc cagctcatcc
    12721 ataaatacct gatggttgtg ctaactcatc accatcaggc attactgtgc aggtggaata
    12781 tccgtcactt caggatgtac gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat
    12841 ggggccatga tttgatccca ggccgcctgc aacgctttca tgttaaattc cggctcgcct
    12901 ttcggctgca gcagtattaa cgccatatca ttcgacaact gctgacgcag atcctgattt
    12961 aacatctcca cagtcaggct gttaaggaaa tcctgccgca gtttctgata ctgctccggc
    13021 gcgatatcga cgacctgatt ttgcaacgaa cgcatccgct ggcccatcag aatatcggta
    13081 tcagcccgtg cataggcggc aaacagtttc tgcagctcca gtttcttttg cgccactaac
    13141 gcattgaact cttcttccgg cagaccttta tcgcgaacct tcgccagttc acgcgccacc
    13201 agattaaggt tgctgttcag cttgtcgttt ggtgattcga tgttgatggc acactgcgca
    13261 cgcagataca gcacacggca gtcaaatcca agaccgatgt ctttgctgtt actggcgctt
    13321 aacgcttgct gaacatgcca gaacagcgcc tcacgggcca ggtccgcacg ccagtagcgc
    13381 agcagtgcgg ctgattcacg aatcggctgc cacggcgtat cccacatgat agataaccgg
    13441 tcctgacgca ccgcgtcagt cataatactc accgcttccg cacgcagcgg agaaagcgtc
    13501 ggcaccggag ccggtgtttc acgtttgcct ttcagttcgc caaacgtttt gttgatttgg
    13561 tcgacgaccg agcgcgcatc cacgtttccc accaccagca gcgtcattgc atccggggtg
    13621 taccatttct gatagaaatc tttaattttt tccgcttcaa cgggttgttt cagcggatcg
    13681 gcaggatcgt gacctaacaa ggttgaccct ttcagacgat agcgccacca gccctcttta
    13741 gtatcggcag gccaggttgc caccatgtcc tgactttgca gcgcgtggtt gatggtttct
    13801 ggcgtgatag tcagtttgcc agtggcattt gccagataag agagcgcctc tttcagcaaa
    13861 tcgttacggt tattgggcaa actcagatta aacagcgtgg tgtcataaga gacaattacc
    13921 ggcggcatcg ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg
    13981 tcaaggccac cgctttgcgt tagcgcaata cgagggatgg cgtgactgta accgctctgt
    14041 tgtgtacttt cggcgagcga accggtatta accagcaggc gaatttcaac acgatcgctg
    14101 ggacgctggg gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc
    14161 catgctggat caggctggag cgcatctgcc tgcacatagc cagcagtggc catcatcagc
    14221 aaaccgcccg ctaaaagtcg aatttttgtg ccctgcatgt gaacccctga tcaactatcc
    14281 tggtaataaa aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt
    14341 tagaccgcaa gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg
    14401 tggtaagttc aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg
    14461 cgggcataag tgatgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt
    14521 attcagcgca tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat
    14581 ggtcgctacg ccgttaccga ccaggttagt cagcgcacga gcttctgaca taaagcggtc
    14641 gataccgagg atcagcgcca gacccgctac cggcaaatgg cccaccgcag agagcgtcgc
    14701 cgccagcacg ataaagccac tacccgttac ccctgccgcc cctttagaag aaagcagcaa
    14761 cacgattaac agcgtgattt ggtggacgat atccatctga ctgttagtgg cctgggcgat
    14821 aaacaccgcc gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt
    14881 cgggatgacc agccccacca ccgatttacg gcagccgagt ttctccatct tgtcgagcat
    14941 acgcggcagc gccgactcgg aagatgaagt ccccagtaca atcagcagtt cttcacggat
    15001 gtagcggata aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag
    15061 caccacaaac aggatacagg taatgtagaa acagataatc agctgcccca gttgcaccag
    15121 tgtgccgacg ccgtatttac cgatggtaaa cgccattgcc ccgaacgcac caataggtgc
    15181 cagacgcatg atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatgacgtt
    15241 aaaaatcagt tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa
    15301 cagcagcacc tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac
    15361 atccataatg aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc
    15421 tttcgcatca agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac
    15481 gatgataaga ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac
    15541 tgcgccggta cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt
    15601 acaaaagatg acaggagcga tgatcatctt aatgagctta acgaagccgt cgccaagcgg
    15661 tttcatttgc tcgcctattt caggatagaa atggccaagg agaataccaa tggctatcgc
    15721 tgtcaggacc tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt
    15781 aaaaccacag gtcttgtaag gttatggggt acctgcggcc ttaaaataac acccagacaa
    15841 catcacagaa atgtacctgg atcataattg aaacaaaaag gttaaaaagt ttgagctggc
    15901 tcgcacaaac cagcactttt taaagttttg taatcagttt ggggtagcta cttttcttcc
    15961 aggtaactct cttcgaagat ttcaataggg agtggacgag caaaaaggaa gccctgggca
    16021 ataccaacgc ccgcttttgc cagccagtcg cgttgtgctt cagtctccac gccttcggca
    16081 atcatttgta agttcaggct ctgcgccagc atgataattg cagcaatcat gctgctatct
    16141 tccggcaagc cttcaacaaa cattttgtcg attttcagaa cgtcgattgg caacgatttc
    16201 atatgctgca gctgacgcag ccctgcgtag cccatgccga aatcatccag cgccacccga
    16261 actccggcat tacgcagcgg acggaggatt gccaccgcag catgagggtc gtcaatacgt
    16321 cggctttctg tcacttccag aatcagtgtt cccggctgaa tgcgatagcg ggttaacagt
    16381 tccagcatat ccgccaccat attcgggtgc atcagttgca gcacagagag gtttaccgac
    16441 aagggcagca taatgccgcg ctcttgccag gctgcaagca atcgacagga ctcttccagc
    16501 acccagtgac cgacggtaac catcagccca cagcactcaa tgcgatcgat taagccatcc
    16561 ggcaggtccc aactgccatc cggttgctgg atacgcagta acacttccgc actgaccagt
    16621 ttaccgctgg tcatctcgac ctgtggctgt aaccaaatag caaactgatg attttccagt
    16681 gcattaagga tatcgctctc ttccgtcaac cgcttctggg cggcttccat ctgctgcgga
    16741 tcaaagaact gaatctgatt cttgccttta tggcgagcgg taaatgccgc agaaatagcg
    16801 cgactgtaaa gctgttcggc ggtgagatcg ccgtagaaca tcgccacgct aatgctacag
    16861 tgcggacgga gttgaatacg ttcaatcggc aggcgctcgc tcatgatagt gagcacttgc
    16921 tgacccaaag tgattgcgtg ccacggttca tgtacaccgt tggcaatgac agcaaagtca
    16981 taaccgctaa tctgcgcgag gatcatacgt ggcgacagta ccgatttgag tttttccacc
    17041 agcgtcagca gcagaatttc tcgttgcgcc tctttcagca cgcccgcagt atcacgcaga
    17101 gtttcacagg tgatgatcat cagcgcggtg gtttgtttac gcgcgacaac ctgctccagc
    17161 atctccatca gcaaggcttt gttcggcaaa tccgacaccg ggaagcgcat cgcattttcg
    17221 ttctgttctt cataatggcg ctgcagcaat tgctggttga ggttgtaact gcgcaccaac
    17281 ataccgattt catcgtcctg atgcagacgc ggtaatgcca gttggtgacc aacaagctcc
    17341 tgggctggga tggcgttaag ttcgcgagca atattgcgta acggatgcaa aatcaggcgg
    17401 ttaatgcacc agctaatcgc caccgtcaat attaacgaca aaagtaagta aatggtcact
    17461 aacgttgaga gggtgctcat cacgaactta tacatacgga aggaatccgc ctgtagcacc
    17521 agataggcaa ttggctgcgg atttgccgga cgttcgagcg agtagacgcc cagcgagatt
    17581 tgaaccggta gctcaaacag gcgagtaacc attaccggca ccggacgctc tggaataaaa
    17641 cttttacgca gcgcctggaa ctggttaggc agcactacat cggcacggct gaccacgcct
    17701 gccggtttaa tgctggcaag aatagcttcc gcttcgggaa tatcaccttt caaaatggct
    17761 gaagataaag gttcgcggac agagcgagca atgctttcca gttgcgtagc cgtgttatag
    17821 cgattctgct ggaccagatg gaacagcaaa acggtgcaaa aaataaaaac gaacaccagg
    17881 acaacggctg ccaccattgc catctgcttg attgttaacg agcgacttac gcgcaaattg
    17941 tctccccccg gaaatcccag attgccgccc gagtataccc gatcgcagcg gcattaagag
    18001 aggcgctatc tgaaaactta ccagtcggcg taaggtatca gcggctgcgg cggtaaatcc
    18061 atgtcaccct gccatccggc ggcggaataa cgtacgtaga gcaggaaatg gctgggggcg
    18121 taatctttcg cctgctggat atcaattgcc gtgccgacaa accagttgga agtaacacgt
    18181 cgttcaagta atgcccgcgc cgtatagccg aagccctgac tgctgccgcc atcgttggat
    18241 tgccgcgcag cttcttcctg ccagtcggtc gggatcagat tcatcagcgg ataacgcggc
    18301 atggttttgg tgcgtgaatg cgaccacgag ccagacgccc ccagctccca cgaccagttt
    18361 tccgtgcgct cccgccacat caccggtatg gcaaacgaca ggtattcctg cgggctgtag
    18421 taaccgccct gaccgagtga gtagccactc aggtctttgt cgtaatgcca gatcatgttg
    18481 ttcaggccga ttgtgacgcg gcgattgttc tggttgatga ccttatagta atagcccgtc
    18541 atccagcgca cacgccagtt atcttcgaca tttttaccgg ttaactggtc gccactaagc
    18601 gatgcccaga cgccgtttgc ttcaccttta tcgtagctca ggcttagccc cacgccgtcg
    18661 gcacgcacgc cgccccattt tttcccggta ttgctcggga agtctttttg cccaccaaag
    18721 gccagcaaag aactggagat gggacgacgg tgggcgttaa cggtgtaacc cagcggcccg
    18781 atatcatcgc tgtaactgat gccgccgacc acatccacca cgttgaagcc catcggcgtg
    18841 gtgccgatat cccagctcca gacgtcattt cgccagccga ccgccacgct ggcaccggaa
    18901 tccgactggc tgcggttgcc gctacagtcc cgtaatgtac aggtgcccca gttgtcatcc
    18961 catttgccat cggcattagt ggagaaactg ccgacgttca tattgacgaa atcactgcga
    19021 aagaacatcc gcccgtcaga atacggcgca tccacctgca acatggtagt gtgcgctttc
    19081 agatcggagt aaccaccggt gccgctcgaa ccccaataat catgctcgag ggtgacgtta
    19141 agatcctgct ggcgatagag gtccgccgca tcgctgcgca cgccgcgttt cagccagtca
    19201 tctttctcgt cgttacgggt cagtcgggta aaggtgtcgt tatcctgcgg acgcgtcgta
    19261 gtcacaccgg atgcgaccat ggcgtctttg taggtttcca gcgcctgctt tggatcgccc
    19321 gcctgcgctt caaacttcgc accgtcgcgc agcaccatcg cgctttccat cgacggtggc
    19381 tgagattttg cctgcgggat caacttatta aacgtctgct gcgccgctgc ggtatctcca
    19441 agctgcgcct gcgccagcgc cacgcgccgc tgtgtgttca gcgaggcgtt atcagtagcg
    19501 ggcagtttcg ccagctggct acgtgccgcc gctgtgtcac cggcagcaat atccacttcc
    19561 gtcagaccaa gaatggcatc ggcgttagtt ggctcccgcg tcaggacatt ctgatatgca
    19621 gcgcgggcgg cggtgtaatc acgtcgctgc agcgcccagt cagccagcgt gaggtcaata
    19681 cgcgtggaag gtggttgctg acgcagcatc gcttccgctt cagcttcttt gccgctttct
    19741 cgcaggcgat tagcggtttc cagcacctga tcgctttgca gtcgattaac cagctcctga
    19801 atattgctgc tccactgcgc acgcggcagg ctgttgatat gcgccagcgc cgctctgtcc
    19861 tggtcatgac cagagaggta cagcccgtaa gcgtaaacct gctccgggtc gttcggcttc
    19921 tgctgcgcca gattgcgcat taacgtatcg gcctggctgc gttgtccggc ctgccagaga
    19981 tcctgcgaaa gtcggtaagt aatccatacg ctgccggggt ccagcgccag tcgttgccgc
    20041 tgaagtgctg ccgcctgcgc ccatttgccc tggttttcca gtgcctctgc ctgctgtgcc
    20101 agacggtcgt tttgcaggct gcgttcgata tcatcaatgc tacgccgctg actggcagag
    20161 agtgaggcga taaacgcttc agctttttct ggcgattgct ggcggtaaat atttgccagc
    20221 ccgcgcacgg cgttagtgtt gccgctgtcc atacgcaagg tctgctgata ataacgttcg
    20281 gcggcgggat aatcttttcg cgccatcgcc acatcgccca gccccagcac tgcataactg
    20341 tcggtgttat cgacattacg cgcctgctgg aacaggcgtt ctgcccggtc aggattattg
    20401 gctttcagcg cagcatcgcc ctgctggatc gccagccagt agcggtttac tttcagcaga
    20461 ctgttccatt tgtcgttgtt gctgctgtgc ggatccagtg cgagggcttt ttccagattc
    20521 gccactgcat tggcgcgatc gcctttctgt gaatatgcct ggcccagcgc ccccagagct
    20581 tcactgtctt tcgggtttgc ccgcactgcc tgttgtagtt cgggaatggc tttacccgcc
    20641 ataccagagt ccaccgccgc taaaccttgc gcacgagcgc ggaaagcagg atcggccagc
    20701 tgtttttgct gttctgccag ttgcgattgc gcagccgcca cgctatcgcc atcactaaag
    20761 atagaaagat attttttcag cgccgacaca ctggcatcac tgacgggcat gtctttaatc
    20821 tgcccgtacc agattttaga ggccccttcg cgcccggcgt tcgatttcgc catctgttcc
    20881 aggacggcaa aaccttcgtc acggcgatcg ctactaaaca gcaatagcgc cagattgttt
    20941 tgcaggcccg tattgcccgg ttcatccgca ttgatgcgtt ttaactgatt aatcgcttcg
    21001 ccacggcgag ccggaatttt cgccaccgta ctccagtact cgacagcaat gtcaccttcc
    21061 ggcggcgcac cgttgaacag ttcgccacag cttcttccgc atgaccggta gtcgcctgca
    21121 atcttgcctg ttgcagtgcc tgacgaccat ccggcgtgga aagtagcatc gtagtccgcg
    21181 acgatttata cgcatttgaa ctcggcgcta actgcgacag ccgatcgagc tgtttttgcg
    21241 cgccatcaat atcgccctga cgtaacaaag aacggaaacg ggcggcaacg acgtccgggt
    21301 tattcggatc aataagttcc agccgataca acgactgctg caccaggtct tcacgatggg
    21361 tcgcttcgcc taaccgaact tgctccagca actgttgctg agcggttggt gctgcctcga
    21421 ccatcggcat gacggccaga ccgagggaaa gcgtgaatat gtttagtgtg aatttgcgca
    21481 ttcctggccc cagtcaggta ataactcacc ttttgtcgag aagcggaaac ggtgttgatc
    21541 ccagccttgt ccaaacaggg tcagcacata gttgtaatag gcatcgctgc cgggaaagtt
    21601 atcggccacg cgctggcgct gaacggcctg cgcatcacgg ttttgtaaaa agggcagcat
    21661 ggcggcagaa aaaccgaccg gtcctttacc ctgcgctttc cccgtagcca catccacttt
    21721 ttccggcgga taaccgtttt tctcagtgaa tgtcgccatc ggtttaaacc ggttgagcat
    21781 ccgcgctttc tgcggatcgc tgtcaggcat catgcctacc cacatgtaaa cgcggatagc
    21841 gtcgtagctg ctgatcaatg ttttttcggc ttttagctgc cagcctttgt ctttctcata
    21901 gcgcacccag tctggcgaaa agcctttcgg ggcggtttcc agcaataaac gttgattggt
    21961 ttcgcgcagc gtagtccacg gcgcgccaaa gcgggtgaaa tactgcgcca gcgtcggcgg
    22021 caggtagctg gggttaaaac gccagctgtt atcctcagca aaacccactt tgcccggtaa
    22081 caacatggaa cccagcccag gcaccgtcac cacttcctcc cgcgcaatac gttttagcaa
    22141 cgcgctgccg atgtcggtat aacgctgctc tttccacaaa cgccccgcct ccagcaacga
    22201 ccaagccatc cagacatcac catcggaggc cgaattgctg tccagcactt cccacttact
    22261 gttctctttc ttgccccaca gccaggcggg caaacgttct tttaaagaac cctgagcgag
    22321 attgttctgc gtccagtcga gaatattatc gaaagctgca cggtcgttag ccgccagggc
    22381 aaagaacatg ccgtaacttt gcccttcaga ggtggtgatt ttgcgcgcgt cgctggggtc
    22441 gatgacgcgc ccttcctgac tgatgtaatc ctttttaaac tgctcccagg caggccaggt
    22501 acaggctgcc tgaacactaa aggcagccaa cagcagcatc gtcacgagcc cactacgcaa
    22561 cacattcatc ttcaattact cgttatccgg gttaagacga cgacggctga taatacgcag
    22621 caaacgccac agtacccatg ccagcaatat cacactgata gccgccagca ccgccagcac
    22681 cgccagcacc gccagcaccg ccagcagaat cggatggttt gccagcgcat accacaagcg
    22741 ctcgaaccac ggcagatgac ctacgtaata aacgtcgcca acacgtaggc tgttgatacc
    22801 ggactcgcgg atcaccgcga ccgaaccgaa catggtggcg cgtttgccgc tatcgttcac
    22861 cgcatcgtta agcatttcat aaccgcgtgg gctatctgcc agcagcgcaa tcacgctgcg
    22921 ctggtcgtta tacggcgact ggaagccaat caccgccgcc atcgcaccgg aagaggtcag
    22981 cgttgactgg gtttctgccg cgcgatcgct ctcgtccggc acaatgccgg ggaacggggt
    23041 ctggcgcatc ggtgttttca cccagctttc ggtcgcctgc accaataggt cgatctgctt
    23101 atcgtctttc agtttgtccg ggataccacc gatgatcatg atgtcggcat ctttgccctg
    23161 aatggtgctg ccatcatcgg tcaccgtcag attaatcgcc gggaagcccg tctgtgcgcc
    23221 gataaaacca acagtattca gcaacgtttc catctgtgct tcgttaggcg ttttcggcat
    23281 cacggtgatg gtttgcgaca gatccgccat ccggctgaac gggaagcccg cgttagcaaa
    23341 ggcgcgtaga tccggcatcg ggatgaagtg gtaatacttc gagaagtcga tggtggagtc
    23401 gtcaccaatc accacatgat tctgcaccgg ctggaaggta atacagttat ccaccgaacc
    23461 gccaggcatc gggttcatat actcaaagtc gaagcgcagc tggttggtcg cgcccagttt
    23521 cagcgccgga atagagacat ccgttttgcc atccagcaaa ccttgtaata ccggaatccg
    23581 cagcagcagg cggttcgcct cctgtttgct gctcaggttg aaggattgca ggaactggtt
    23641 attcaggctg atatccatcc gcgaactgtc tttcaccggc ggcatggtgt agcggtaatt
    23701 aatatccata tcaatgccgg tactgcgcat caggtagaga tctggcggca ggtttagcga
    23761 aacattaatc gctgctggct caagaccgct ggattgtaac tgttcttcat aggttttcag
    23821 ttcaccaaag gtgaccggac gatcggtacg cacccagttc ggcgcatcgt acggcttacg
    23881 cggtagcagc ggtttcactt cattcactac cacgctttca ccacggaaca gaatgttacc
    23941 ctgagcgata cctttcgctg cctgcaacag gtctttgtcg tcacgaccaa acaccaccag
    24001 cagtttgacg taaggattct gcggatggtt aatcatctca atcaccgggg cttttaccgc
    24061 cggatgatcg cgcaggaagt ccggccgttt gtcgttggtt gcaaagacaa tggcattacg
    24121 atccggcagt tggttataga gcaccgggaa gttctgccca cgccagccag aacgcgaacc
    24181 aaaccacgag gcgacaatgg cagatgcttg ttgcagccca acatccggcg cacccgcaaa
    24241 gaccatcggc aaggtgttgg tgcggttatc acgcgggtca aagaatggca ccgggaagtg
    24301 tgacaggtca ttcttcacat tcagggtctg ataggtcaga tccagtccac tgctccgccc
    24361 aacatccagc caaagcgtgg tgctggccgg gttttcgcac acgtcctgat aatggccgac
    24421 aaactccagc cgcacacggt tgaagtcggt aataaacagt ggattaatgg gcatttgcgc
    24481 cagcgttttt ttacccaact gttctttggt cactggcagc acgcccatca gctcatcatt
    24541 gagataaacc tttaactgcg actggacagg cagtaacgat ggcgatggcg tgtattcgag
    24601 gttgagcatc gccttcgtca ccacttcatc gctgcgcata ccaaactcaa tgctgccgtt
    24661 cgggttaatg ccacgtagca ccatgctgcc cggcggcggc gcaatttgcg caaaggtcag
    24721 cttcacatca cgcgaaggac cgttctgcgc cacgactggc gcatcagcgc cctgcacgcc
    24781 cggcatcact tgccccacct gcggattttg ttccgtctgg gcggctacag ctggctcagc
    24841 attgatcagt ggttgcgttg ctggcgtcgc ctgcgtcatg aaagagggga acgcactcat
    24901 ccccatagcc actgcacaaa tccagaatag ttttcttttc atcgcgttat catcattgtt
    24961 gagccaaagc ctgatccgat ggttgtgccg tttcgctccg ctccgggcgg cgcggaataa
    25021 acgatacaac ccaggaaacc agagaagtca gcacacggaa tatgcccttc accgaagaag
    25081 gcgcaaactc cgccagatgg cggtagccac ggaagccgag cttcagaata tccagcagac
    25141 tttccagcgg cttatcttcc gggtagctgt cctgccagag cgcccatgta tccgcacggg
    25201 caaacgtaca ctgcacaaaa tcgatatgtt gctgggtggt gagcggcatt aatttcagcc
    25261 caacttcatt acccatcacg cgcgccacct gggtcgggaa gacgtattcc tgctgaccgc
    25321 gtttaagcaa cagattcact ttctgccctt ccagaatctg cgcctgaccg ttgatcttga
    25381 tccccaaacc accgtcggag aaatcctgaa cggtacacga gaagaggtga ccatcttcgc
    25441 gggcaattgc cgcgggcatc gtcatctcca cgcggtgcga tcggcgtacc tgtttgcttt
    25501 ctaccgatac cgcaactgcg ccgccaagaa caatcaggtt gtagaatacc cacaccatac
    25561 tgacgaccac ggtgagcatc tcggttggcg ggccatagaa gtagcgccag atgcctaccg
    25621 caacgcccac caggttgagc agaacaagga agatgtaggg ccgcgagatc acccagtcga
    25681 cgtactcttc ttccaccagt ccacctttgg cggtgacgtt aaatttgcct ttgtgcgggt
    25741 taatcagcgc caccagcgtc ggtggtgcga tataccacgc cagcaccgtt tcgtagattt
    25801 cactccagaa agagtggcga tatttgccct ggatcttgga gttggtcagg ctggcatgga
    25861 tcatatgcgg cagcacgaac agggcgatca tcaacgctgg cgcatagatg atgtaggcat
    25921 gaagcagcag gaacgccagc ggcgcagtca ggaagatcag ccgtggaatg cccgacaaga
    25981 agtggaacat ggcgttgacg tagcacagcc gctgagcaaa cttcagccct ttaccggtga
    26041 gcgggttatc gagacggaag atttgcacca tcccgcgcgc ccagcgaata cgctgaccga
    26101 tatgcgccga cagactttcg gtcgccagcc ccgccgcctg cggaatacgc atatacgcgg
    26161 aggtatagcc acgacggtgc aaacgcagag aagtatgcgc atcttcagtc acagtttcga
    26221 cagcaatgcc gccaatttca tccagcggct tacggcgaat caccgcacag gaaccgcaga
    26281 agaaagtggc gtcccacata tcgttgccat cctgcaccag accatagaac agcgtgcctt
    26341 cgttcggtgt tttacgaaaa cgccccaggt tgcgttcaaa cgggtccggc gagaagaaat
    26401 ggtgtggggt ctgcatcatc gccagctgtt tctctttcag gaaccagccc acggtcattt
    26461 gcaggaacga tcgcgttggt acgtggtcgc agtcgaaaat cgacacgaac tcgcctttgg
    26521 catatttcag cgcattgttg atgttgcccg ctttcgcatg ttcatgagtg gtacgggcga
    26581 tatacttcac ccccacgttt tgcgcaaact ggcgaaactc ttccctgccg ccatcatcga
    26641 ggatccagat gttcagcttg tctttcggcc agtcgatacc cagcgaggcg taaatggtat
    26701 ttttcaccac gttgagatct tcgttgtaag tcgggacaaa gatatccacc gacggccaca
    26761 gcgacatatc tttcggcaat ggcaccggct gacgattcag cggccatact acctggaagt
    26821 agccgagcac cagcacaatc cacgcgtacg tttcagcgaa gagcagaata agcccgcaca
    26881 ccaggctgac cggatcgtcc cagttcagcg tagaggtata tcgccaccag atataacggc
    26941 aagaaacagt cagcgacagc acaatcaaca ttagcgctga gaagcgcccc ggcatccgcc
    27001 gtacgatcag cgctcccccc cacagcagca tcaggaagat aaactgcgcc agcgggttaa
    27061 acggctgagt aacgcagatt aacgccagaa tcagcgagaa ggtgacgatg ataccgagga
    27121 tcaaccgccg cgccccggca ctcaaatgac cgagctcttt tttctcatca agatgctgtg
    27181 ttttatggct aacgcgctca ggcagctcgt tcatccattg atggtaacgt ccacgaatat
    27241 tctgcagacc tgaaaatgcc cgcctgcgcg gtttcggcgt ttctttgcgc gatgcaccga
    27301 tcagtaacca gcatgtttga atgagataac ggaccgggtc cagcggacgc ggacgcgagg
    27361 cgttgatatg cggatacagg tttttatgtt ctgcgcgaat acgctgccag cgcgggtgct
    27421 ccagtggaat aaaaatccag gccaggatca tccagaaaca gccgagcgtc gcgctgaaag
    27481 ccgacgcacc gtgacgacga taatcgcgat aacgcccgat aagccgcgcg ttgaccggcg
    27541 ggataagcaa ccaccgggtc aggatactca tgatgcactc ccgactggcg ttttcagccc
    27601 ggaatagttc aacaggcacc agttcgccag cgtcaatatc tcttcagccg ccagcgcatc
    27661 actgcgatat tcacctactg gttgcttagc cgccaggcat tcagccatcg cttcatcacg
    27721 atgaatgagc atcggcagta atcggcgctg gctttgcaac caaagctggt aaatatcgtc
    27781 ctgaacctga ctgccaatac ggaagttatt aatcaaaata tgtgcgccat ccggcagcgc
    27841 ttgctgatgc agtcggatat ggcagttggc atccacattg acgattgcca gcgagtgatc
    27901 gcacaaactc agcagctggt gggttatctg cgaggcatca cgcggtaagt cgattaaaat
    27961 ccactggtaa cgcccgctgg cttttagttg ctgtaagccg gagcaaatat cgctcagccg
    28021 ggtttgccag tgctgtggat tttcttgttc ttcaatggat aactgaccaa aaggcagcaa
    28081 atcgagctgc gaggtgtagc gcaacccagc gtcgcgccag tcctagccat ccagcatagc
    28141 tctggcccag ccctgacggt gggtaaaatc aacattaaat gacaggcgca acaagttgtc
    28201 cgggcaggca tcgaccacca ggacattttc tcccaacatt tgtaatgacc aggctaatgc
    28261 ggcgg
//
LOCUS       DAZKIF010000057        27585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZKIF010000057 DAZKIF010000000
VERSION     DAZKIF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.057"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(246..1838)
                     /gene="lsrK"
                     /locus_tag="ACHOTW_002403"
     CDS             complement(246..1838)
                     /gene="lsrK"
                     /locus_tag="ACHOTW_002403"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFP9844501.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIVE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1917..2870)
                     /gene="lsrR"
                     /locus_tag="ACHOTW_002404"
     CDS             complement(1917..2870)
                     /gene="lsrR"
                     /locus_tag="ACHOTW_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP9844502.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3119..4654
                     /gene="lsrA"
                     /locus_tag="ACHOTW_002405"
     CDS             3119..4654
                     /gene="lsrA"
                     /locus_tag="ACHOTW_002405"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFP9844503.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            4648..5676
                     /gene="lsrC"
                     /locus_tag="ACHOTW_002406"
     CDS             4648..5676
                     /gene="lsrC"
                     /locus_tag="ACHOTW_002406"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP9844504.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5676..6668
                     /gene="lsrD"
                     /locus_tag="ACHOTW_002407"
     CDS             5676..6668
                     /gene="lsrD"
                     /locus_tag="ACHOTW_002407"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP9844505.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6680..7702
                     /gene="lsrB"
                     /locus_tag="ACHOTW_002408"
     CDS             6680..7702
                     /gene="lsrB"
                     /locus_tag="ACHOTW_002408"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFP9844506.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7729..8604
                     /gene="lsrF"
                     /locus_tag="ACHOTW_002409"
     CDS             7729..8604
                     /gene="lsrF"
                     /locus_tag="ACHOTW_002409"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFP9844507.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8628..8918
                     /gene="lsrG"
                     /locus_tag="ACHOTW_002410"
     CDS             8628..8918
                     /gene="lsrG"
                     /locus_tag="ACHOTW_002410"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFP9844508.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8975..9733
                     /gene="tam"
                     /locus_tag="ACHOTW_002411"
     CDS             8975..9733
                     /gene="tam"
                     /locus_tag="ACHOTW_002411"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP9844509.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9737..10651)
                     /gene="yneE"
                     /locus_tag="ACHOTW_002412"
     CDS             complement(9737..10651)
                     /gene="yneE"
                     /locus_tag="ACHOTW_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP9844510.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
     gene            complement(10848..12299)
                     /gene="uxaB"
                     /locus_tag="ACHOTW_002413"
     CDS             complement(10848..12299)
                     /gene="uxaB"
                     /locus_tag="ACHOTW_002413"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9844511.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12526..13944)
                     /locus_tag="ACHOTW_002414"
     CDS             complement(12526..13944)
                     /locus_tag="ACHOTW_002414"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9844512.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14083..14442)
                     /gene="yneG"
                     /locus_tag="ACHOTW_002415"
     CDS             complement(14083..14442)
                     /gene="yneG"
                     /locus_tag="ACHOTW_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP9844513.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14442..15368)
                     /gene="glsB"
                     /locus_tag="ACHOTW_002416"
     CDS             complement(14442..15368)
                     /gene="glsB"
                     /locus_tag="ACHOTW_002416"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP9844514.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15432..16820)
                     /gene="sad"
                     /locus_tag="ACHOTW_002417"
     CDS             complement(15432..16820)
                     /gene="sad"
                     /locus_tag="ACHOTW_002417"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFP9844515.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16921..17802
                     /gene="yneJ"
                     /locus_tag="ACHOTW_002418"
     CDS             16921..17802
                     /gene="yneJ"
                     /locus_tag="ACHOTW_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9844516.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17880..18995
                     /gene="yneK"
                     /locus_tag="ACHOTW_002419"
                     /pseudo
     CDS             17880..18995
                     /gene="yneK"
                     /locus_tag="ACHOTW_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            19146..20336
                     /gene="ydeA"
                     /locus_tag="ACHOTW_002420"
     CDS             19146..20336
                     /gene="ydeA"
                     /locus_tag="ACHOTW_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP9844517.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20361..21026)
                     /gene="marC"
                     /locus_tag="ACHOTW_002421"
     CDS             complement(20361..21026)
                     /gene="marC"
                     /locus_tag="ACHOTW_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP9844518.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            21238..21672
                     /gene="marR"
                     /locus_tag="ACHOTW_002422"
     CDS             21238..21672
                     /gene="marR"
                     /locus_tag="ACHOTW_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP9844519.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21692..22075
                     /gene="marA"
                     /locus_tag="ACHOTW_002423"
     CDS             21692..22075
                     /gene="marA"
                     /locus_tag="ACHOTW_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP9844520.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22107..22325
                     /gene="marB"
                     /locus_tag="ACHOTW_002424"
     CDS             22107..22325
                     /gene="marB"
                     /locus_tag="ACHOTW_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP9844521.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(22356..23255)
                     /gene="eamA"
                     /locus_tag="ACHOTW_002425"
     CDS             complement(22356..23255)
                     /gene="eamA"
                     /locus_tag="ACHOTW_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP9844522.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            23450..24637
                     /gene="ydeE"
                     /locus_tag="ACHOTW_002426"
     CDS             23450..24637
                     /gene="ydeE"
                     /locus_tag="ACHOTW_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP9844523.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(24678..25073)
                     /locus_tag="ACHOTW_002427"
     CDS             complement(24678..25073)
                     /locus_tag="ACHOTW_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9844524.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            25168..26139
                     /gene="ftrA"
                     /locus_tag="ACHOTW_002428"
     CDS             25168..26139
                     /gene="ftrA"
                     /locus_tag="ACHOTW_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HFP9844525.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            26247..26342
                     /gene="mgtS"
                     /locus_tag="ACHOTW_002429"
     CDS             26247..26342
                     /gene="mgtS"
                     /locus_tag="ACHOTW_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP9844526.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            26342..26446
                     /gene="mgtT"
                     /locus_tag="ACHOTW_002430"
     CDS             26342..26446
                     /gene="mgtT"
                     /locus_tag="ACHOTW_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP9844527.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(26561..27451)
                     /gene="dgcZ"
                     /locus_tag="ACHOTW_002431"
     CDS             complement(26561..27451)
                     /gene="dgcZ"
                     /locus_tag="ACHOTW_002431"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP9844528.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         7015 a         6956 c         6632 g         6982 t
ORIGIN      
        1 taaaaatcga agtttattaa accccttttg catccattaa tgtctttagg aatattcgtt
       61 ataaaataag ggttatgctt aatgctttaa gaaaaatagc aatttcccct tgaatatagt
      121 actggtgatg aaacgatgaa tctgcaagct ggcatttaac aagccagctc taaaagaagg
      181 gaaataagaa taactatact caaaaactaa cagccacggt catcatgatg tggctgtcaa
      241 tgaaactata acccaggcgc tttccataac gacgtcgtca gtccatgatc aaccagcccc
      301 agctgatcct gataaactgc ctgccactta tcgcgtgaat cctgataaag ttcatgcttt
      361 tccgggtctg gtgtgtgcgt ccgttcccag cgaaccaggc gttctccggt ttctgccatt
      421 gatgaaaaaa ttccggcacc gacgccagct gcaatggcac atcctaatgc agtggcttct
      481 ttgaccaccg gaatattgac gggtaatccc gagacatcag cgagaatttg actccataat
      541 ttcccttttg aacctccgcc tgcaaagact aacgatgaag gatgaatagt cgaaaaatca
      601 gcaatttgct gcaagttaca cgctgataca atcgccgcat tttcttccag cgcacggaac
      661 aatgtcgctt tgttacattt atccggatca atggacaaat taataaagga aggcgcagcg
      721 tgataccagg ttttaaagcg cattctgtcg gagaagatcg gcattacgcc ccacgaccca
      781 ggcggcaccc gactggccat ctcttccagc agcgtatagg tgtcgatgcc taaacgttcg
      841 acaatcagtt tttcttcggc acagaaagca tcgcggaacc agcgcatggt gagtccggta
      901 aaaaagctta tagattcagc ttgtaccatg ccaggaataa catgaggatt aacgcgcacg
      961 ttcatttctg ggtctgtcac cggcgcggct aaatttacaa cttgctgcca gaatgtgccg
     1021 ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc ccaggcaacc aagctgcacg
     1081 tcgcctcctc caacgaccac cggagtgccc accttcagac cgcagagttc cgccgcttgt
     1141 gaacttacca cgcccagcaa tgtgccggtt tctttgacag gagaaagaat atcggcacgc
     1201 aggccagcca tatccagcaa tgcaggtttc cagtcacgga tggttagatc aagaagtccc
     1261 gtggtgccag cgttagaggg atccaccgca agttcgccgc tgagcatata ggccagccag
     1321 tcgctgatca tggtgatggt tgatgcctga cggtaaatat cggaacgatg gtgcgccagc
     1381 caaagtaatc tggggatggc acttaaagcc agtgtttgtc cggtcgcgcg ataaacttcg
     1441 ttttcaaagg tattgttgtg cagttcttta agttcgctaa cttcgcgcgc cgctctggca
     1501 tccacattgg cgcaggccca gatcggtgtt ccttcattgt tatataaaac aatgccttca
     1561 cgcatcgaac atgccgaaac ggcagcgata tactccgggg ctatgccggc gttgtgcagc
     1621 gcctggcgca tacactcaca cgccagttgc cagtttttgt tgagatcaaa ttccatagaa
     1681 ccaggaacgt ccggtactgc cagatgccgc cactccgcct gtcccactgc tatttgattg
     1741 ccttccaggt cgaatatcac agcccgaata cttccggtgc ctgcatccag cgccatcagg
     1801 tagtactttg attctgaagg ggtaaagagt cgagccatta gattatcctc ggcttatgcg
     1861 ttctgtctgt tcctctatac gttctccatc attcccggta ataaggtcat gcaaatttaa
     1921 ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca gtgcgttgat ataaccgcct
     1981 ttcattgcag cggcaattgc ttcggcttta ttttctcccc ctgccacgcc aacccggacg
     2041 ggtatggtct tcagcgcgct taaaggtaag ccaatcagct cgttatgtat tttaatgtcc
     2101 gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa tgtcgccaac cgcccctttt
     2161 cggccaatca ttaactgttc gccctggctg atataaccgg agcgaatgat cgtcgcgtcg
     2221 tcttgttgac taacggcccc aatgccgaca atcgccacat ccgctgcttg cgcggctaac
     2281 agaacatctt tgacacaatt ttcatttttt agcgtacggg caatgtcagc ggaggatgcc
     2341 cgcaacggag ccggaataat attcacactg cacgccgcat taagctgtcc gatacccgtc
     2401 atataagaac cgacgccacc ggagagcgtg accaggcgaa tttgctgtga cgaaataaaa
     2461 ccacttaagc gttgcagcgt attcatggtt gcctcgccaa aaccaatcgc cagcatctgt
     2521 tgtggttgaa gtaaactcat caacatatgc gccgcgccta tccccagtcg cccgccgaca
     2581 tcagcatccg caagcccagg gatcacccgg acatgttgca gcgaaaactg acgacgtaat
     2641 tgtgtttcat attccagaca gccttcaaag cgagaattaa tctgcacgcg aatgatgccg
     2701 gactgatgcc ctttctccag taatcgcgac actttcaaac gggtcaggcc gagacgatcg
     2761 ctgatctcac tctgggtcag cccgtcgtgg tagtaaaacc acgcgatccg cgcgacctgt
     2821 tcttcttcac acattccctg ttctgaaatt accgaatcgt tgattgtcat aattcattct
     2881 tcactttgaa catatttaaa tctttaatgc aattgttcag ttcttgcgca tttatatctg
     2941 tgatggcaac cacagtttga ctctacgagc atgaacaaac gcaaccgtga aaatcaaaat
     3001 agtataaatt gtgatctatt cgtcggaaat atgtgcaatg tccacctaag gttatgaaca
     3061 aattaaaagc agaaatacat ttgttcaaaa ctcacctgca aaactgaacg ggggaaatat
     3121 gcaaacgagt gatacccgcg cgttaccgct actttgcgcc cgctcggttt ataaacagta
     3181 ttcaggggtc aatgtcctga aaggcatcga ttttacgttg catcaggggg aggtccacgc
     3241 cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg aagattattg ccggtattac
     3301 ctctgctgat agcggtacgc tggagattgg gggcaacaac tacgccagat taacgccagt
     3361 tcatgctcat cagctgggta tttatctcgt tccccaggaa ccgctgcttt tcccaagcct
     3421 gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa cagctctcca tgcagaaaat
     3481 gaagaacttg ctggcggcgc tgggctgcca gtttgatctg catagtctgg caggatcgct
     3541 ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg ctgatgcgcg actcgcggat
     3601 tctgatcctc gatgaaccta ccgcctcgct tacccctgcg gaaaccgaac gcttgtttac
     3661 tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt tttatctcgc ataagctgcc
     3721 ggaaattcgc cagattgccg atcggattag cgtgatgcgc gacggaacca tcgccttaag
     3781 cggcaaaacc agcgaactgt ctaccgacga cattattcag gccatcaccc cagcggtacg
     3841 ggaaaaatcg ctctctgcca gccaaaaatt atggctggag ttacctggca accgcccaca
     3901 acatgccgca ggaacgtcgg tgctgacact ggaaaatctg actggcgaag gtttcaggaa
     3961 tgtcagcctg acgctaaatg ccggagaaat tctgggtctg gctgggctgg tgggtgccgg
     4021 acgcacagaa ctggccgaga cgctctatgg tctacgtact ttgcgtggcg gacgcattat
     4081 gctgaatgat aaagagatca atagattatc cactggagaa cgtttactgc gcggtctggt
     4141 ttatctgccg gaagatcgcc agtcatccgg actgaatctc gatgcttcgc tggcctggaa
     4201 cgtctgcgcc cttactcata accttcgtgg attctgggcg aaaaccgcga aagataatgc
     4261 caccctggaa ctttatcgtc gggcgctgaa tattaaatat aaccaaccgg aacaagctgc
     4321 acggacatta tccggtggta accagcaaaa aatcctgatt gccaaatgcc tggaagcctc
     4381 gccgcaagta ttgattgtcg atgagccgac gcgcggcgtg gatgtctcgg cgcgtaatga
     4441 tatctaccaa ctgttgcgca gcatcgccgc acagaatgtg gctgtgctgc ttatctcctc
     4501 tgatctggaa gagatcgaac tgatggcaga tcgtgtgtat gtgatgcatc agggcgaaat
     4561 tacccactct gcactgaccg ggcgcgatat taatgtcgag actattatgc gcgttgcctt
     4621 cggcgatagt cagcgtcagg aggcggcatg ctgaagttta ttcagaacaa ccgtgaaatc
     4681 acggcactgc tggcggtgct gctgctgttt gtattacccg gttttctcga ccgccagtat
     4741 ttaagtgtgc aaacgctgac catggtttat agcagcgcgc aaatcctgat cctgctggca
     4801 atgggcgcga cgctggtaat gcttacgcgc aatattgatg tttcagtggg ttcgattacc
     4861 ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag gatattcact acctgttgcc
     4921 tgtgtcgcga ctttactgct tggtttgctc gcgggatttt tcaacggtgt cctggtcgcg
     4981 tggctaaaga tccctgccat tgttgccacc cttggcacgt tagggttgta cagaggcatc
     5041 atgttgctgt ggactggcgg taaatggatt gaagggttac ccgccgaact gaaacagctc
     5101 tccgccccgc tgctgcttgg cgtttcagca attggttggt taacgataat tctggtggca
     5161 tttatggcct ggctgctggc gaagacagcg tttggacgca gtttttatgc cacgggcgat
     5221 aatttacagg gcgctcgtca actgggcgtt cgtacggaag ccattcgcat tgtggcattt
     5281 tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt ttgcttcgca gattggtttt
     5341 atccccaacc agaccggtac cgggctggag atgaaagcaa ttgcagcctg cgtgctgggc
     5401 ggcattagtt tgctcggcgg ttccggtgcg atcatcggtg cggtactcgg cgcatggttc
     5461 ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc cggcatggtg gaatgatttt
     5521 atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg gacgcctgcg ttgtgcgctg
     5581 gaacttaatc tgcggcggca aaaatatgcc cgctttatga cgccaccgcc atccgttaaa
     5641 cccgcttcgt caggtaaaaa acgggaggct gcataatgcg tattcgctac ggttgggaac
     5701 tggctcttgc cgcactgctt gttattgaga ttgtctcatt tggtgcaatt aacccgcgga
     5761 tgttagatct caatatgttg ctgttcagca ccagtgactt tatctgcatt ggcattgtcg
     5821 ccctaccgct gacgatggtg attgtcagtg gcgggatcga tatttcgttt ggttcgacca
     5881 tcggcctctg cgccattgca ttgggcgtac tgtttcaaag tggtgtgccg atgccgctgg
     5941 cgattctcct gaccttactg ctcggcgcat tgtgcgggct gatcaacgcc ggattaatta
     6001 tctataccaa agttaacccg ctggtgatta cgcttggcac gctgtatctg tttgccggaa
     6061 gcgccctgct gctttccggg atggccgggg cgacggggta cgaaggtatt ggtggattcc
     6121 cgatggcgtt tacagatttc gctaacctgg atgtgttggg gctccccgtt ccgctgatta
     6181 tcttcctgat atgtctcctc gttttctggc tctggctgca taaaacccat gccggacgta
     6241 acgtgttttt gattgggcaa agcccgcgcg tggcgcttta tagcgcgatt ccagttaacc
     6301 gtaccttatg tgcgctctat gccatgacgg ggctggcgtc tgcggtcgcc gctgtgctgc
     6361 tggtgtcgta ttttggttca gcacgttccg atctcggtgc gtcgtttctg atgcccgcca
     6421 tcaccgccgt ggtgcttggc ggtgccaata tttatggtgg ttccggtgcc attatcggca
     6481 ccgccattgc ggttttatta gtgggatatt tgcaacaagg tttgcaaatg gcaggagtgc
     6541 caaatcaggt gtccagcgcc ctttccggtg cgctacttat cgtcgttgtc gtaggtcgtt
     6601 ccgttagcct gcatcgccag caaattaaag agtggctggc gcgtcgggcc aataacccat
     6661 tgccataaag gatatcttca tgacacttca tcgctttaag aaaatcgcct tacttagcgc
     6721 tcttggcatt gccgcaatct ctatgaatgt gcaggccgca gagcgtattg catttattcc
     6781 caaactggtt ggcgtgggat tttttaccag cggtggcaac ggcgcacaac aagcgggtaa
     6841 agagctgggc gttgatgtga cctacgacgg gccgacagaa cccagtgttt ctggtcaggt
     6901 acagttgatt aataacttcg tcaatcaagg ttataacgcc attatcgttt ctgcggtttc
     6961 gcctgatggc ttgtgtccgg cactgaaacg cgccatgcaa cgtggtgtga gagtgctgac
     7021 ctgggactct gatactaaac cggagtgccg ctcttactac attaatcagg gaactcccgc
     7081 ccagttggga ggtatgttgg tggatatggc ggcgcgtcag gtgaataaag acaaagccaa
     7141 agtcgcgttt ttctactcaa gccccaccgt tacggaccaa aaccagtggg tgaaagaagc
     7201 gaaagcgaaa atcgccaaag agcatcctgg ctgggaaatt gtcactacgc agtttggcta
     7261 taacgatgcc actaaatcat tacaaaccgc agaaggaata ttaaaagcgt atagcgatct
     7321 cgacgccatt atcgcccccg atgccaatgc cctgcccgct gccgcacaag ccgcagaaaa
     7381 cctgaaaaat gacaaagttg cgattgtcgg attcagtacg ccaaacgtga tgcgtccata
     7441 tgtggaacgc ggcacggtga aagaatttgg cctgtgggat gtggttcagc aaggcaaaat
     7501 ttcagtttat gtcgcggatg cattattgaa aaaaggatca atgaaaacgg gcgacaagct
     7561 ggatattcag ggcgtaggtc aggtggaagt ctcgccaaac agcgtccagg gctatgacta
     7621 cgaagcggat ggtaatggca tcgtactgtt accggagcgc gtgatattca acaaagagaa
     7681 tatcggcaaa tacgatttct gatgtgcatt acttaaccgg agtaagttat ggcagattta
     7741 gacgatatta aagatggtaa agattttcgt accgatcaac cgcaaaaaaa tatccctttt
     7801 accctgaaag gttgcggtgc gctggattgg ggaatgcagt cacgcttatc gcggatattt
     7861 aatccgaaaa cgggtaaaac cgtgatgctg gcttttgacc atggttattt tcagggacca
     7921 actaccggac ttgaacgcat tgatataaat atcgccccgc tgtttgaaca tgccgatgta
     7981 ttaatgtgta cgcgcgggat tttgcgcagc gtagttcccc cggcgaccaa taagccggtg
     8041 gtactgcggg cgtcaggtgc gaactctatc ctggcggaat taagcaatga agccgtggcg
     8101 ttatcgatgg atgacgccgt gcgcctgaac agttgcgcgg tggcggcgca ggtttatatc
     8161 ggcagcgaat atgaacatca gtcgatcaaa aatattattc agctggtcga tgccggaatg
     8221 aaagtgggaa tgccgaccat ggccgtgact ggcgtgggca aagacatggt gcgcgatcag
     8281 cgttatttct cgctcgcgac tcgaatcgcc gctgaaatgg ggacgcaaat tatcaaaacc
     8341 tattatgtcg aaaaaggttt tgaacggatt gttgccggat gtccggtacc cattgttatt
     8401 gctggcggta aaaaattacc ggagcgcgag gcgctggaaa tgtgctggca ggctatcgat
     8461 cagggcgctt ctggtgtgga tatggggcgt aatattttcc agtctgacca tccggtggcg
     8521 atgatgaaag ccgtacaggc ggtggttcac cataacgaaa cggctgatcg ggcatatgaa
     8581 ctctatctga gtgaaaaaca gtaactgcgg atttaaggag aagaattatg cacgtcacac
     8641 tggttgaaat taacgttcat gaagacaagg ttgacgagtt tatcgaagtt tttcgccaga
     8701 accacctggg ctctgtacag gaagaaggca atttgcgctt cgatgtctta caggacccgg
     8761 aagtgaattc gcgcttttat atctacgaag cctataaaga tgaagacgca gtggcgttcc
     8821 ataaaaccac gccccactac aaaacctgtg tcgcgaaact ggaatcttta atgaccgggc
     8881 cgcgtaaaaa acgtctgttc aatgggttga tgccgtgagg cgaatttatc agttttatct
     8941 acaattgggg taacgcgctg acgggagtaa ataaatgtct gactggaacc cctctttata
     9001 tctgcacttt gccgctgaac gatcgcgtcc ggcggtggag ctgcttgcca gagtgccgct
     9061 ggagaatgtc gattatgttg ccgatcttgg ctgtggccca ggtaacagca ccgccctgct
     9121 gcatcaacgt tggcctgctg ccaggataac gggcatcgac tcgtctccgg cgatgattgc
     9181 tgaagcgcgc agtgctttgc cagactgcca gttcgtggaa gcagacatcc gcaactggca
     9241 accggaacag gcactcgatc tgatttttgc taatgcctca ctgcaatggc tgcccgacca
     9301 ctacgaattg tttcctcact tggtttcttt acttaatccg cagggcgtgc tggcagtaca
     9361 gatgccagat aactggctgg agccgaccca tgtgctcatg cgcgaagttg cctgggaaca
     9421 aaactacccg gatcgcgggc gtgagtcgtt ggctggtgtt catgcttact acgatatttt
     9481 gagcgaagcc ggatgtgagg tcgatatctg gcgaaccacc tactatcacc agatgccgtc
     9541 gcaccaggcg attatcgatt gggtgaccgc cactggatta cgtccgtggt tacaggatct
     9601 gaccgagagc gaacagcagc tttttcttac gcgctaccat cagatgctga aagagcagta
     9661 tccactgcaa gagaacggac agatactgct ggcatttccg cgtctgttta ttgttgcccg
     9721 ccgtacggag taaatatcac gtcagttggt aatggcgatc gggaagaact tttgctggaa
     9781 tttcggcttc atcgttcatc tgtaagaggt caatttcaat agcgttacag atggcatcca
     9841 gcggtaaatc attgttttca gtaccgaacg gatcttccag ttcttccgcc agacaatcca
     9901 gcgaaataaa agtataggaa atcagcacag agataaaagg cgtcatgtaa tgcaggtcca
     9961 cgaccagcgc gaacggcagc atgatacaaa acagataaac ggtacgatgc aaaatcaggg
    10021 tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcgca gcctgccaga accgctgaaa
    10081 tatcgttaag ccgatcgttg aggctaataa acaggatatc tgaaagttgt ccattgcgtc
    10141 gctgaaccgc caaccactcc cccattatta acaagatgcg gttagctgga gagttcgaag
    10201 ccagtacacg ctgaagatct tcagtcttga gataatgagc cagcacttcc acctgtggct
    10261 gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat ttgcagccgg gcaaactccc
    10321 ttacacttgc cgaatccggc aatgtcgttt ttacctcgcg cagtaacgac cgtgaggcaa
    10381 tcatcaactg cccccaaagt ttgcgcgctt caacgtaacg ggcgtaaccg gcattattac
    10441 gaaaaccaag aaaaatggcg atggcgacac cgagaatgct gaacggtgcg agggtgaatt
    10501 tgatgcccag atgcgtgtac cagggcagca tgaaaataac agcgatagaa aagagaaaat
    10561 tgagtagtaa gcgcgaggat atcttggata atactgagcc atgccagaca aaaatacggc
    10621 gcagccagtg ttgttgtgga cgaacaatca tggttatctt caggcgtgga aaagtcgccc
    10681 tattaaacgt gattacgatc acattctcaa gacactactt acaaattacc tacaaaatta
    10741 caacattaac aggtgctgca tgtggcacct gttaatgata aaggcttata ggatgttgta
    10801 actaatataa tgaaataaga aacccggtcg aaaccgggtt cagagtatta gcacaacgga
    10861 cgtacagctt cgcgcatccc tttttcgaga atcgcatcca ggtcattagc cacctgctcg
    10921 accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc caacacaatc
    10981 gctaccagtt cctgggtacc gataacacgg tcacgatgct gactccagag ttgctgataa
    11041 cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccattacg ctcaccgcga
    11101 tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt gccgtttgcc
    11161 ttctgccctg ccagcagctg cggcaggatg cgggtacgga acttggtcat accgttgagc
    11221 gcgatagaca gcagctgatg cttaatgtac gggttacgga aacgcccggt gactgcactg
    11281 gcgaaagatt ccagttcatc gcgtggcaaa tccagtaccg gaataatttc ttcgtagatg
    11341 gctttttcaa cgaatgcgca aatttcagca tcgttcatcg cttcacctac ggtatccagc
    11401 cctgcctgga acgccaccgg caccaacgcg gtgtgtgcac cgttgagtat tgccactttg
    11461 cgttctttat acggtttaat atcgtcaaca atcagcacgt tgagcggata tttgtccaga
    11521 cgcagctcgg tcgctaagga tttcggtccc tgaatcacaa acaagtaaaa gtgttcagcg
    11581 gtgtcgagga aaccatcgtg ataacccagt tcttcttcca gtttagccac ttcatcacgc
    11641 ggataaccgg taacgatacg gtccaccagc gtagaacaga agctgttagc ctgatccagc
    11701 cattgaataa atgcttctgg cagtgcccac tcttgcgcat agcgcagcac cagttcacgc
    11761 aacgcgtcgc cattgtagtc gatcaactca cacgggatga tgatccaacc tttatccagc
    11821 gcaccgttga aatggctaaa acgttcgaac agcagacggg tcagttttgc cggataactt
    11881 accgctggcg cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc ttcggtggtg
    11941 ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt tcaggaattc atcgtattca
    12001 ctgtagacgc tgatttcacg attaacagag cgaatcagac gcgcgtcgct gaccgcttcc
    12061 cccttctcat tcaggccacg gataatggtg gtgtacagac catcctgcgt gctcagtgac
    12121 ggcgggaatg aagtttcaat cggacgaaca acgaccacac cagaattcag atcggtgtgc
    12181 tcattcagga gatctatttg ccagtcaaca aaggcgcgca ggaagttacc ttcaccaaac
    12241 tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc gacgatttag tgttttcaca
    12301 atgggttccc ttctgattag tcatacaacc tgtttgaatt ggtacgacag gttagcaaac
    12361 tttaatacga cgaacccctg ttttgatcaa ctcctgatga ttaatgagca gttttatgag
    12421 aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg ccttttcctg gatcataaag
    12481 tggtagaaca cattgcattc aaatcgcgcg gaatgaataa agatgtcaga caacttcctc
    12541 accgtaacgc atagtgctgg tacggttgcg cccatctttc ttcgagcgat acagacaggt
    12601 atcggcttcg accatcaatt tattaaagtc atcggtcagc gttcggtacg atgcgcgacc
    12661 actaccgacg ccaatactta ccgtgagata aagcgttttt tgttgccagg tgaatggttg
    12721 cagttcaacg cctttacgga ttttttccgc cattagcaga ccatcgacag gattcactga
    12781 cggcactgca acagcaaatt cttcgccgcc catgcgcgcc accagcccct tatcaccgac
    12841 aatcttctga atatgccggg caaacacgct taacacttta tcgccacatt catgtccata
    12901 gttatcgtta atacttttga aatagtcgat atcaagtagc atgacagtca gatgttgtgt
    12961 ctgtttcagc gatggacttt tcagcgcctc ataaagaccg gaccgtgagt aaacctgagt
    13021 cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt agcgaattga tcgctgccac
    13081 gctaaaagaa accataattg ggcatatcgc catcgtggca ataccgagac gtgcggagaa
    13141 catttgcgga atggagaacg gcgaaccgac cgagatatca atcaccgaat tagccaccag
    13201 tacgatttcc accgcaccgg tgacaaaggt caacagacat gttacctgcg gcgtatagcg
    13261 cactgcacac cagattaatg caggcagcgg gaacgccaga ctccccgctc cgccaatgac
    13321 taccgaggca ataaccgaca caataagcgc gatggcgggc atcatctgtt ctgctttaaa
    13381 gcgcggcagc actccaggaa tagccaacgt cagcatacaa ggcacgatca acacgcccgt
    13441 tgagaattgc tcactgaacc agtccgcaag caaaggccag aaatccagac tatcaatact
    13501 gaccgaacca atcgccccga caatagcgca taataatgcg gcaatcagac agtaattaaa
    13561 tagccgtaag gcacttactg gctcatattt atttttcccc aaacgcttat cccgcacgac
    13621 cagtaaggcg acggtaacaa taaacatcat attggagaaa ttgatagcca gtgaaaccag
    13681 tccccattcg gtggttatgg catcataaac cagcatcgcc acataactga tcgcatagta
    13741 atgcagtcga ttaagccaga catagcgggc aaacaccccc gccattacgc cattcagcgg
    13801 ccagaaaaga gaaagttcgt gaaccagccg taactctgca ccaataaaat agaacagcgt
    13861 ggttagcgca aaaatagcga tgctattacg tagcagatga ccttcctgga acacacggaa
    13921 agttgaaatg gggtgtacat gcattaatta tctgtccata caattgcggg tggcaattat
    13981 ctcaacgtaa taagtaaatt ctcataaagg atagcatgtt cgctgacagc ggcggcaaat
    14041 aaaggtaccc tgggaatgct attttgtcgt tttccgcaat tctcatggct gattcatctg
    14101 tacaaccagc cagtgataga ttaccgccac gatataacgt tgttgttctt cgcttagcga
    14161 aacaccatgc gggatgttat gccacttagc cagacaaccg cgacagcagg tcgcggtggc
    14221 gtgctgagcg ataaacaccg gatgcccgcg catgggggtt tgcttaccgt cattagccgg
    14281 taatgccgga gcaagacgtt tagcgacaaa atccgccgca tgttgttcga tgactggcgc
    14341 gcctttctcc agacaatact gacgctcttt catgcccaaa cgaaagcgag atcgaaattt
    14401 tgaacgggat aaacgcgcga agagtggatc gagagactgc attaataaac cgaacgccct
    14461 aactgtttcg tcaattgttc aaggacggcg atacccgcaa gcgagttacc tgcatcatcc
    14521 agttccggac tccagactgc gatggccatt tcatgcggaa caatcgccac aataccgcca
    14581 ccaacgccag atttcgccgg tagtcccacc cgccaggcaa actcccccgc gttctggtac
    14641 ataccactgg tcgccatcag cgcgttaatt tgccgtgcct gcattggcgt caccacaggt
    14701 tcatcaatat gaatagcttt cccctgatta gccagaaaga caaacgtccg ggccagctct
    14761 acacagctca ttttcagagc gcagtaatga aaatagtttt gcagaacggt tgtcacgtca
    14821 tgatggaaat tgccaaacga cttcatcagc caggcgatag ccgcatttcg cgcggaatgt
    14881 tcaaattcgg aacgcgctac caccgtatcg taggaaatat cagacacacc gcttaagccg
    14941 cgcacgactt ccagcatacg ttgccgtggt gcgcttaatc gcccttgcaa catatcgcag
    15001 accaccagcg caccagcatt aataaacgga ttacgcggaa taccctgctc catttccagt
    15061 tgcactaaag aattgaacgg tgatccagac ggatctttgc cgacgcgttg ccagatttcc
    15121 tcttcggagt aatgacgcat ggcgacaacg agactcagca ctttggaaat agactgaatg
    15181 gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt acagatagca
    15241 atgcccaatc gggaaccgtc tactgtagcc agcgccggaa tataatccgc gactttaccc
    15301 tgaccaatga gcggccgcac ttgccgcaag atgttttcta aaattgcatt atccatggcg
    15361 actgccactt tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat atcagagctg
    15421 aatatgtcgc gtcagatccg gtctttccac accgtctgga tattacagaa ttcgtgtaag
    15481 ccgaaatggg aaagctcacg accaaagcca ctctttttca cgccaccaaa ggccactcgc
    15541 gcgtcgctgg cacaataacc attgataaac accccaccgc attccagacg tgccgccatc
    15601 tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg aaaggccgaa ctcactatca
    15661 ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg tgattgccgc aacggggcca
    15721 aacatttctt cccgaaaagc ggtcatttct ggggtaacat tcgccagaac cgttggcgga
    15781 tagtagttac ctgccccagc catcttttcc ccgcccagta acaaacgcgc accctgcgcc
    15841 agggttttct ccacctgatg atgcagctca tcacgtaaat cgaaacgagc cattggtccg
    15901 agagcgttct cttcgtcacg gggatcgtcc attttcaagg ctgccgcagc tgccacaaaa
    15961 cgttcggtaa atgccgaagc aattccctct tcgataataa agcgttttgc cgctgcacaa
    16021 acctgtccgg tattctgata acgtccggct accgccgctt taactgccag ttccagatcg
    16081 gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt ccagtacgca ttttttcagt
    16141 gccgctccag cctgtgcacc aatagccgct ccagcacgaa cacttccggt caccgtgata
    16201 gcagcaatgc gcgagtcttt aatcatctga gtgacaccgt cattgtcggc attcagccag
    16261 ccatatacgc cttgtgggat tcccgcatct ttaaacacct gggcaatgag ctgagcacag
    16321 cccatcacat tcggcgcatg tttaagtaag tatccgttac cagcaagaat gatgggaaca
    16381 gcgccacgca tcacctgcca taacggaaaa ttccacggca taatcgccag aatcgtcccc
    16441 aacggtcgat actcaataac tgcctgctga ttttccacca gcgtaggttc cgccttcagc
    16501 attgccggac catgttctgc ataccagtca cacaaattcg ccgatttcgc cacttcagcg
    16561 cgcgcctggt tgattggttt gcccatttcg cgggtgatca tttgcgccat ttcttcgcta
    16621 cgggcgcgca gagccttacc gatatcacgc agtttttcag cacgataatc tatatttgtc
    16681 tcgcgccagt cacgaaagcc tgctgccgcc agctgaagtg cgttttcgat atcatcagcg
    16741 ccagcccacg gcaacacaga aagttgttca cccgtggcag gatttatcga aattgcatga
    16801 gttgccggag taatggtcat cggggtatct cctttatgag tcatggtatg aagatacgca
    16861 gatttactct tgctttaaaa tgaataatat taagccactt attcacgaat cgagaatatt
    16921 atggatctga cccaactgga gatgttcaac gcggttgccg aagctggcag cattacgcag
    16981 gctgcagcaa tagtgcatcg cgtgccgtcg aatctcacta cccgtctacg ccagctggaa
    17041 acagaactgg gggtagatct gtttattcgc gagaatcagc gtttacgtct atctcctgcc
    17101 gggcataact ttttacgcta tagccaacaa attctcgcgt tagtggatga agcgcggagc
    17161 gttgtcgctg gcgatgagcc gcaaggttta ttttctctgg gttcgctgga aagcaccgca
    17221 gcagttcgca ttccggccac gctggcggaa ttcaaccgtc gttatcccaa aattcagttt
    17281 tcactttcca ccggcccttc cggcactatg ctggaaggtg tgctggaggg aaaacttaat
    17341 gcggcgttta ttgatggacc cattaaccat actgccattg acgggatacc ggtataccgc
    17401 gaggagctga tgatcgtcac gccacaaggt catgcgccag taatccgtgc cagtcaggtt
    17461 aatggcagta acatttatgc cttccgcgcc aattgttcgt atcgtcgcca cttcgagagc
    17521 tggtttcatg ctgacggtgc cgctccggga actatccatg agatggagtc ttatcacgga
    17581 atgttggcct gtgtggtcgc aggagcaggt attgcgctta ttccgcgctc tatgctggaa
    17641 agtatgccgg ggcatcacca ggttgaagcg tggccgttag ctgagcaatg gtgttggcta
    17701 acaacctggc tggtctggcg tcgtggtgcg aaaacgcgtc cgctcgaggc atttattcaa
    17761 ctactggatg tgcctgactc gacaaaacag ggttaccaat gagctatttt tgatagttct
    17821 tgcggctaat atgctctata tagtgatgtt ccgatgactt atgactatat ggggcaaata
    17881 tggttacgcc agtaagcatc agcaattaca tatctcttcc tgatgatttt cccgttcgta
    17941 atattgcacc gcaggtaaaa gaagttttaa aggattttat tgatgcactt agtacaataa
    18001 tatgtaatga agagtggcgc acgtctttaa acatcaactc agccacaaaa aagatattta
    18061 ataatcttga caacttatca tatattcaga ggacttcttt tcggggtaac gacacgctat
    18121 acaatgaaaa ggttcagttt aaactcactt atcccgccag gaatggaaga cacaaagaaa
    18181 atattgaatt tcaggtagta ataaatttaa gccctattta tttagataat ttccgccatg
    18241 atggagaaat taatattttt tgcgctccca acccaaagcc tgttactatg gggcgcgtat
    18301 ttcagaccgg cgtcgagcgt gtgctctttc tgtttctgaa tgattttatt gaacaattcc
    18361 caatgatcaa ccctggcgtt cccatcaaaa gagcgcatac accacatatt gaacccctgc
    18421 ctccggatca ccataccgct gcagattact tacgccagtt tgatttgctt gtcctgaatt
    18481 ttatctctcg cggtaatttt gtcatactcc cccgattatg gaataactct gaggttcaca
    18541 gatggttcgt caataaagat cctaacttga tcaccgccat tctcgacata acggacagcg
    18601 aattaaaaga ggatttattg caaagcctga tggattcatt aggttctaac aaacatgtac
    18661 taccctaagt ctgcatctgt tttttatccc ttttagcaga acaagaatct cctcattttc
    18721 aggacttgtt tttatttttc gccaatatgt tgttacacta tcaccaattt atgaatccca
    18781 atgaaagtga tttgaatgac gtgttgatgc cagcatcatt aagtgatgat aaaattatca
    18841 aacatatggc gcgtaagacc ctcaaactgt ttgtaaaaaa tgaaacaccg ccaaaagtta
    18901 ctcatgaaga tctggtgaaa aacaggcctc gctcccctgt cagaccgcct atacccgcaa
    18961 cctccaaaac gccagacatt cctgatcgtc attaaaccgt gatgtttacc atctctctga
    19021 cgcgtgaaag aatcagcgtc agagaaacgg aaaacgcgat ccagatcaca aatgcattgt
    19081 attcacatca ttaaccgttt taagatcatt tcatcacttt ttcgcaactc acccgataat
    19141 ctgttatgac aacaaacact gtttcccgca aagtggcgtg gctacgggtc gttacgctgg
    19201 cagtcgccgc cttcatcttc aacaccaccg aatttgtccc tgttggcctg ctctctgaca
    19261 ttgcgcaaag ttttcacatg caaaccgctc aggtcggcat catgttgacc atttacgcat
    19321 gggtagtagc gctaatgtca ttgcctttta tgttaatgac cagccaggtt gaacggcgca
    19381 aattactgat ctgcctgttt gtggtgttta ttgccagcca cgtactgtcg tttttatcgt
    19441 ggagctttac cgttctggtg atcagtcgca ttggtgtggc ttttgcacat gcgattttct
    19501 ggtcgattac ggcgtccctg gcgatccgta tggctccggc cgggaagcga gcacaggcat
    19561 tgagtttaat tgccacaggt acagcactgg caatggtatt aggattacct ctcgggcgca
    19621 ttgtggggca gtatttcggt tggcgaatga ccttcttcgc gattggtatc ggggcgctta
    19681 ttaccctttt gtgcctgatt aagttacttc ccttactgcc cagtgagcat tccggttcat
    19741 tgaaaagcct cccgctatta ttccgccgcc cggcattgat gagcatttat ttgttaactg
    19801 tggtggttgt caccgcccat tacacggcat acagctatat tgagcctttt gtgcaaaaca
    19861 ttgcgggatt cagcgccaac tttgccacgg cattactgtt attactcggt ggtgcgggca
    19921 ttattggcag cgtgattttc ggtaaactgg gtaatcaata tgcgtctgcg ttggtaagta
    19981 cggcgattgc gctgttgctg gtgtgcctgg cactgctgct acctgcggcg aacagtgaaa
    20041 tacacctcgg ggtgctgagt attttctggg ggatcgcgat gatgatcatc gggcttggta
    20101 tgcaggttaa agtgctggcg ctggcaccag atgccaccga cgtcgcgatg gcgctattct
    20161 ccgggatatt taatattgga atcggggcgg gtgcgttggt aggtaatcag gtgagtctgc
    20221 actggtcaat gtcgatgatt ggttatgtgg gcacggtgcc tgcttttgcc gcgttaatat
    20281 ggtcaatcat tatatttcgc cgctggccag tgacactcga agaacagacg caatagttga
    20341 aaggcccatt cgggcctttt ttaatgattc gttttaatga tttccaggat gccgttaata
    20401 ataaactgta cacccataca taccagcagg aaccccatca agcgggagat cgcttcaatg
    20461 ccacccttgc ccaccagccg cataattgcg ccagagctgc gtagacttcc ccacaaaata
    20521 accgccacca ggaaaaagat cagcggcggc gcaaccatca gtacccaatc agcgaaggtt
    20581 gaactctgac gcactgtgga cgccgagcta ataatcatcg caatggttcc cggaccggcg
    20641 gtacttggca ttgccagtgg tacaaaagca atattggcgc tgggttcatc ttccagctct
    20701 tccgacttgc ttttcgcctc cggtgaatca atcgctttct gttgcggaaa aagcatccga
    20761 aaaccgataa acgcgacgat taagccgcct gcaattcgca gaccgggaat cgaaatgcca
    20821 aatgtatcca tcaccagttg cccggcgtaa tacgccacca tcatgatggc aaagacgtac
    20881 accgaggcca tcaacgactg acgattacgt tcggcaccgt tcatgttgcc tgccaggcca
    20941 agaaataacg cgacagttgt taatgggtta gctaacggca gtaacaccac cagccccagg
    21001 ccaattgctt taaacaaatc taacattggt ggttgttatc ctgtgtatct gggttatcag
    21061 cgaaaagtat aaggggtaaa caaggataaa gtgtcactct ttagctaacc ttgcatcgca
    21121 ttgaccaaaa cttgaaccga tttagcaaaa cgtggcatcg gtcaattcat tcatttgact
    21181 tatacttgcc tgggcaatat tatcccctgc aactaattac ttgccagggc aactaatgtg
    21241 aaaagtacca gcgatctgtt caatgaaatt attccattgg gtcgcttaat ccatatggtt
    21301 aatcagaaga aagatcgtct gcttaacgag tatctgtctc cgctggatat taccgcggca
    21361 cagtttaagg tgctctgctc tatccgctgc gcggcgtgta ttactccggt tgaactgaaa
    21421 aaagtgttgt cggtcgacct gggagcactg acccgtatgc tggatcgcct ggtctgtaaa
    21481 ggctgggtag aaaggttgcc gaacccgaat gataagcgcg gcgtactggt aaaacttacc
    21541 accagcggcg cggcaatatg tgaacaatgc catcaattag ttggccagga cctgcatcaa
    21601 gaattaacaa aaaacctgac ggcggacgaa gtggcaacac ttgagcattt gcttaagaaa
    21661 gtcctgccgt aaacaaaaaa gaggtatgac gatgtccaga cgcaatactg acgctattac
    21721 cattcatagc attttggact ggatcgagga caacctggaa tcaccactgt cactggagaa
    21781 agtgtcagag cgttcgggtt actctaaatg gcacctgcaa cggatgttta aaaaagaaac
    21841 cggtcattca ttaggccaat acatccgcag ccgtaagatg acggaaatcg cgcaaaagct
    21901 gaaggaaagt aacgagccga tactctatct ggcagaacga tatggcttcg agtcgcaaca
    21961 aactctgacc cgaaccttca aaaattactt tgatgttccg ccgcataaat accggatgac
    22021 caatatgcag ggcgaatcgc gctttttaca tccattaaac cattacaaca gctagttgaa
    22081 aacgtgacaa cgtcactgag gcaattatga aaccgctttt atccgcaata gcagctgcgc
    22141 ttattctctt ttccgcgcag ggcgttgcgg aacaaaccca gcaaccgctc gttacttcct
    22201 gtggcgatgt ggtggttgtt cccccatcgc aggaacaacc accgttcgat ttaaatcaca
    22261 tgggtacagg cagtgacaaa tcggatgcgc tgggcgtgcc ctattataac caacaagcca
    22321 tgtagtatat tcaggccccg gcatatcggg gcttgttaac ctcttacctt caccgcttta
    22381 cgccactgca aaccaaatac attgatatac agtccggcca taatgagcac agcccctaag
    22441 aattgcagac ctgttaagcg ttcatccagc agtaacgctg cacttgccag accgactacc
    22501 ggcaccagta atgataatgg tgcgacacgc catgtttcat agcgtccaag cagcgtaccc
    22561 caaatcccgt agccaactat cgtcgctaca aatgccagat acatcaacgc cagaatggtg
    22621 gtcatgtcga tagtgaccag actgtgaatc atttttccgg agccatcgag aatgagcgag
    22681 gcgacaaaaa acggaattat cgggattaat gcgctccaga cgaccagaga cattaccgcc
    22741 ggacgggatg aatgagacat gatctttttg ttgaagatat tgccactcgc ccaactaaat
    22801 gccgctgcca gagtcaacat aaagccgagc atcgccacat gctgaccgtt aaagctatct
    22861 tcgattaaca ccagtacgcc agaaatcgct aacgcaatcc ccgccagttg cttaccgtgc
    22921 agccgttcgc caaaagtaaa cgcgccaagc acgatagtaa aaaacgcctg agcctgtaag
    22981 accagcgagg ccagtccagc cggcataccg aagttaatgg cgcagaagag aaaggcaaac
    23041 tgtgcaaaac tgatggttaa accatacccc agcagtaaat tcagcggtac ttttggtcgt
    23101 gccacgaaaa agattgccgg gaaggcgacc agcataaagc gcaaaccggc cagcatcagc
    23161 ggtggcatgt tatgaagccc cactttgatg accacaaaat taagccccca tacaacgacg
    23221 actagtagcg ccaacacccc atcttttcgc gacattctac cgcctctgaa tttcatcttt
    23281 tgtaagcaat caacttagct gaatttactt ttctttaaca gttgattcgt tagtcgccgg
    23341 ttacgacggc attaatgcgc aaataagtcg ctatacttcg gatttttgcc atgctatttc
    23401 tttacatctc taaaacaaaa cataacgaaa cgcactgccg gacagacaaa tgaacttatc
    23461 cctacgacgc tctaccagcg cccttcttgc ctcgtcgttg ttattaacca tcggacgcgg
    23521 cgccacgctg ccatttatga ccatttactt gagtcgccag tacagcctga gtgtcgatct
    23581 aatcggttat gcgatgacaa ttgcgctcac tattggcgtc gtttttagcc tcggttttgg
    23641 tatcctggcg gataagttcg acaagaaacg ctatatgtta ctggcaatta ccgccttcgc
    23701 cagcggtttt attgccattc ctttagtgaa taacgtgacg ctggtggtgc tcttttttgc
    23761 cctcattaac tgcgcctatt ccgtttttgc taccgtgctg aaagcctggt ttgccgacaa
    23821 tctttcatcc accagcaaaa cgaagatctt ctcgatcaac tacaccatgc tcaatattgg
    23881 ctggaccgtc ggcccgccac tcggcacgct gttggtcatg cagagcatca atctgccctt
    23941 ttggctggcc gctatctgct ctgcgtttcc catgtttttc attcaaattt gggtaaagcg
    24001 cagcgagaaa atcatcgcca cggaaacagg cagtgtctgg tcgccgaaag ttttattaca
    24061 agataaggca ctgttgtggt ttacctgctc tggctttctg gcttcttttg ttagtggcgc
    24121 atttgcttca tgcatttcac aatatgtgat ggtgattgct gatggtgatt ttgccgaaaa
    24181 ggtggtcgcg gttgttcttc cagtgaatgc tgccatggtg gttacgttgc aatattccgt
    24241 gggccgccgc cttaacccgg ctaacatccg cgcgctgatg acagcaggca ccctctgttt
    24301 cgtcatcggt ctgatcggtt ttattttttc cggcaacagc ctgctattgt ggggtatgtc
    24361 agctgcggta tttactgtcg gtgaaataat ttatgcgccg ggcgagtata tgttgattga
    24421 ccatattgcg ccgccaggaa tgaaagccag ctatttttcc gcccagtcat taggctggtt
    24481 gggcgcagcg gtaaatccat tagtgagtgg tgttgtactt accactctcc cgtcgtcttc
    24541 gctgtttatc attctggcgt tagtcatcat tgccgcgtgg gcgctgatgt taaaaggtat
    24601 taacgccaga ccccagaggc aaccagcact ctgttgacgt tcgtgccgtt ccactactgg
    24661 cacggcttcg ctaatcatta ttctgaggca aaggcatagc cttcatcttc ccagccggtt
    24721 atcccgccga gcatgatttt aaccggcttt ccgagtcgag ccagtttgag tgccgcacgg
    24781 tctgcaccgt tacaatgtgg ccccgcgcaa tacaccacaa atagcgtatc gtcgggccat
    24841 tcggccattc tttctgcggt catttgctta tggggtaaat ggagagcgcc tgggatatgc
    24901 cgacgcgcga agtcctcttc acgtccaacc acatgaagta aaacgaaatc atcgccaata
    24961 taagccagag agtcatgaac atcggcgcaa tctgtttcga cactcaagcg cgcgagaaaa
    25021 tgcgcctcag caacttctgg cttcgctgtg gtaaattcag tgacataacg catattgctt
    25081 tgtccttttg gtttgttgtg ttaacaataa tgtaactaat ctacctttca ctgaccgcct
    25141 ctcgatatgc cagaaatgag caaaatgatg acaacgttac aacgtccgct ggtcattgca
    25201 ctggcatatg acgggctatg tacttttgaa tttggcgttg ccgttgaaat attcggcctg
    25261 ccacgaccgg aactgggcga taactggtat cgttttgctg ttgctgcggt agaaccaggt
    25321 gcgctacaag ccaccggggg gatccacgtg gttgctgatg gtggactgga gttattgccc
    25381 aacgccgata tgattattat tcctggctgg cgcggaatag atgccaaagt gccggagatg
    25441 ctatgccagt cattgcgcga ggcacaccag cgcggtactc gcctggtatc aatatgctct
    25501 ggcgtgtttg tccttgctgc tgcgggcctt cttcatcagc gaaaagcaac cacccactgg
    25561 cgctacactg aacaactcca gcaccgttac ccttcaatca gcgtaagtga caatgtgtta
    25621 tatcaggatg aaggacaagt gatgacctcc gccggaagcg ccgcggggat cgatctgtgt
    25681 ttacatatcg tgcgcaaaga ttttggtcat gagattgcta ataatgtcgc ccggcgactg
    25741 gtcattcagc cacatcgcca gggggatcaa ccacaaaatc tgacgcgccc aatggcctca
    25801 ccgcgtgaaa gccagacgtt aggtgcctta ttcgactttt tgcagcagaa tctggcacaa
    25861 acacatactg tgtcttccct cgccgaacgg gtaaatatga gtccgcgcac ttttttaaga
    25921 cgttttgagg cgttaactgg catgacgccc gcccgctggc tgctcaatga gcgcttaata
    25981 cgagtacagg attatctgca aaattcggtt tttaacatcg agaaaattgc cgagctgacg
    26041 ggttttggca attcagcgtt attgcgtcat cactttcacc aacgtttttt attatctcct
    26101 tcgcaatatc gcaaaaacag ccgttcccca tcacaataat cgcctgattc agcctttgtt
    26161 tagataacct ctgctaatat ctttctcatt gagataaaaa ttaaggtaag cgaggaaaca
    26221 caccacacca taaacggagg caaataatgc tgggtaatat gaatgttttt atggccatac
    26281 tgggaataat tttattttct ggttttctgg ccgcgtattt cagccacaaa tgggatgact
    26341 aatgaacgga gataatccct cacctaaccg gccccttgtt acagttgtgt acaaggggcc
    26401 tgatttttat gacggcgaaa aaaaaccgcc agtaaaccgg cggtgaatgc ttgcatggat
    26461 agatttgtgt tttgctttta cgctaacagg cattttcctg cactgataac ggatcgttga
    26521 cacagtagca tcagttttct caatgaatgt taaacggagc ttaaactcgg ttaatcacat
    26581 tttgttcgtc aataaacatg cagcgatttc ttccggtttg cttaccctca tacattgccc
    26641 ggtctgctct tccaatgacc acatccagag tctcttcagg aaatgcgcga ctcacacctg
    26701 ctgtcacggt aatgttgata tgcccttcag aatgtgtgat ggcatggtta tcgactaact
    26761 ggcaaattct gacacctgca cgacatgctt cttcatcatt agccgctttg acaataatga
    26821 taaattcttc gcccccgtag cgataaaccg tttcgtaatc acgcgtccaa ctggctaagt
    26881 aagttgccag ggtgcgtaat actacatcgc cgattaaatg cccgtaggta tcattaacca
    26941 atttaaatcg gtcaatatcc aacaacatta aataaagatt cagaggctca gtgttgcgta
    27001 actgatgatc aaaggattca tcaagaaccc gacgccccgg caatcccgtc aaaacatcca
    27061 tattgctacg gatcgtcagc aaataaattt tgtaatcggt taatgccgca gtaaaagaaa
    27121 gcaccccctc ctgaaaggcg tcgaaatgcg cgtcctgcca gtgattttca acaatagcca
    27181 gcattaattc ccgaccacaa ttatgcatat gttgatgggc agaatccatt agccgaacgt
    27241 aaggtaattc atcgttatcg agtggcctca gatgatcaat ccaccgacca aactggcaca
    27301 gtccataaga atggttatcc gttatttctg gcttactggc atctctcgcg accacgctgt
    27361 gaaacatact caccagccac tgatagtggg catcgatagc cttattgaga tttaacaaga
    27421 tggcatcaat ttccgttgtc ttcttgatca ttgccactcc tttttcacag ttccttgtgc
    27481 gcgctattct aacgagagaa aagcaaaatt acgtcaatat tttcatagaa atccgaagtt
    27541 atgattcatc tctgagataa cattgtgatt taaaacaaaa tcagc
//
LOCUS       DAZKIF010000058        27417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZKIF010000058 DAZKIF010000000
VERSION     DAZKIF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.058"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            109..867
                     /gene="ydiY"
                     /locus_tag="ACHOTW_002432"
     CDS             109..867
                     /gene="ydiY"
                     /locus_tag="ACHOTW_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP9844529.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(920..1027)
                     /gene="yniD"
                     /locus_tag="ACHOTW_002433"
     CDS             complement(920..1027)
                     /gene="yniD"
                     /locus_tag="ACHOTW_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP9844530.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1202..3100)
                     /gene="espL1"
                     /locus_tag="ACHOTW_002434"
     CDS             complement(1202..3100)
                     /gene="espL1"
                     /locus_tag="ACHOTW_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFP9844531.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENIFITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSIGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSMSFS
                     ADELKTYL"
     gene            3623..5551
                     /gene="thrS"
                     /locus_tag="ACHOTW_002435"
     CDS             3623..5551
                     /gene="thrS"
                     /locus_tag="ACHOTW_002435"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9844532.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            5555..6097
                     /gene="infC"
                     /locus_tag="ACHOTW_002436"
     CDS             5555..6097
                     /gene="infC"
                     /locus_tag="ACHOTW_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9844533.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            6194..6391
                     /gene="rpmI"
                     /locus_tag="ACHOTW_002437"
     CDS             6194..6391
                     /gene="rpmI"
                     /locus_tag="ACHOTW_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9844534.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            6444..6800
                     /gene="rplT"
                     /locus_tag="ACHOTW_002438"
     CDS             6444..6800
                     /gene="rplT"
                     /locus_tag="ACHOTW_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9844535.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    6917..7041
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6923..6967
                     /gene="pheM"
                     /locus_tag="ACHOTW_002439"
     CDS             6923..6967
                     /gene="pheM"
                     /locus_tag="ACHOTW_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP9844536.1"
                     /translation="MNAAIFRFFFYFST"
     gene            7250..8233
                     /gene="pheS"
                     /locus_tag="ACHOTW_002440"
     CDS             7250..8233
                     /gene="pheS"
                     /locus_tag="ACHOTW_002440"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9844537.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            8248..10635
                     /gene="pheT"
                     /locus_tag="ACHOTW_002441"
     CDS             8248..10635
                     /gene="pheT"
                     /locus_tag="ACHOTW_002441"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9844538.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            10640..10939
                     /gene="ihfA"
                     /locus_tag="ACHOTW_002442"
     CDS             10640..10939
                     /gene="ihfA"
                     /locus_tag="ACHOTW_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP9844539.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            11040..12020
                     /gene="btuC"
                     /locus_tag="ACHOTW_002443"
     CDS             11040..12020
                     /gene="btuC"
                     /locus_tag="ACHOTW_002443"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP9844540.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            12083..12634
                     /gene="btuE"
                     /locus_tag="ACHOTW_002444"
     CDS             12083..12634
                     /gene="btuE"
                     /locus_tag="ACHOTW_002444"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP9844541.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            12634..13383
                     /gene="btuD"
                     /locus_tag="ACHOTW_002445"
     CDS             12634..13383
                     /gene="btuD"
                     /locus_tag="ACHOTW_002445"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP9844542.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            13461..13925
                     /gene="nlpC"
                     /locus_tag="ACHOTW_002446"
     CDS             13461..13925
                     /gene="nlpC"
                     /locus_tag="ACHOTW_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9844543.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            14172..14885
                     /gene="ydiV"
                     /locus_tag="ACHOTW_002447"
     CDS             14172..14885
                     /gene="ydiV"
                     /locus_tag="ACHOTW_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP9844544.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            14948..16384
                     /gene="selO"
                     /locus_tag="ACHOTW_002448"
     CDS             14948..16384
                     /gene="selO"
                     /locus_tag="ACHOTW_002448"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP9844545.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(16388..16579)
                     /gene="hemP"
                     /locus_tag="ACHOTW_002449"
     CDS             complement(16388..16579)
                     /gene="hemP"
                     /locus_tag="ACHOTW_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP9844546.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(16711..17757)
                     /gene="aroH"
                     /locus_tag="ACHOTW_002450"
     CDS             complement(16711..17757)
                     /gene="aroH"
                     /locus_tag="ACHOTW_002450"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP9844547.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(17914..18747)
                     /gene="ppsR"
                     /locus_tag="ACHOTW_002451"
     CDS             complement(17914..18747)
                     /gene="ppsR"
                     /locus_tag="ACHOTW_002451"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP9844548.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            19080..21458
                     /gene="ppsA"
                     /locus_tag="ACHOTW_002452"
     CDS             19080..21458
                     /gene="ppsA"
                     /locus_tag="ACHOTW_002452"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP9844549.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(21515..23161)
                     /gene="fadK"
                     /locus_tag="ACHOTW_002453"
     CDS             complement(21515..23161)
                     /gene="fadK"
                     /locus_tag="ACHOTW_002453"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP9844550.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFTPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCSCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(23218..23511)
                     /gene="ydiT"
                     /locus_tag="ACHOTW_002454"
     CDS             complement(23218..23511)
                     /gene="ydiT"
                     /locus_tag="ACHOTW_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9844551.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(23508..24797)
                     /gene="ydiS"
                     /locus_tag="ACHOTW_002455"
     CDS             complement(23508..24797)
                     /gene="ydiS"
                     /locus_tag="ACHOTW_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9844552.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(24853..25791)
                     /gene="ydiR"
                     /locus_tag="ACHOTW_002456"
     CDS             complement(24853..25791)
                     /gene="ydiR"
                     /locus_tag="ACHOTW_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP9844553.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(25811..26575)
                     /gene="ydiQ"
                     /locus_tag="ACHOTW_002457"
     CDS             complement(25811..26575)
                     /gene="ydiQ"
                     /locus_tag="ACHOTW_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP9844554.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            26891..>27417
                     /locus_tag="ACHOTW_002458"
     CDS             26891..>27417
                     /locus_tag="ACHOTW_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP9844555.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGY"
BASE COUNT         6833 a         6507 c         7105 g         6972 t
ORIGIN      
        1 cgcgcctttt tataattcgt tacctttcca gggacgctaa tctttgatcc ttaagaaagt
       61 ttccgggacc gcgatggtct ctttttttgt tgtacggact cttgataaat gaagcttttg
      121 aagacagttc ctgccatcgt tatgttggcg gggggcatgt ttgcgtcact gaatgcagct
      181 gccgatgatt ccgtttttac tgtcatggac gatcctgcca gcgctaaaaa acctttcgaa
      241 ggtaatctga acgcagggta tcttgcacaa tcaggcaata cgaaaagctc ctcacttacg
      301 gctgacacca ccatgacctg gtatggacaa accactgcct ggtcgctgtg ggggaacgcc
      361 agtaatactt cttctaatga tgagcgttct tcagaaaaat atgcggcggg cgggcgtagc
      421 cgttttaatt taactgatta tgactattta tttggtcagg caagctggct gacagaccgt
      481 tataacggct atcgagagcg cgatgtgttg accgcgggtt atggtcgtca gtttttgaat
      541 ggcccggtac acagtttccg ctttgaattc ggtccaggtg tgcgttacga caaatatacc
      601 gacaatgcca gtgaaactca gccgctgggc tatgcctccg gtgcttatgc atggcagctt
      661 actgacaacg ctaaatttac gcagggtgtt tccgtctttg gcgcggaaga cacaacgctg
      721 aactccgaaa gcgcattaaa tgttgctatt aatgagcatt ttggactgaa ggtggcttac
      781 aacgtcacat ggaactctga acctccagag tctgcaccgg aacataccga ccgtcggaca
      841 accttatcac tgggctacag catgtaaaat tatctgccgg accgtgttat gcggtctggc
      901 tgtcttcgtt tgtcactgct cagccccaaa tcattgctgc ccaacgtagc aacaacatag
      961 cgccacagat tgccagtagc accaccacca tcttccagtg ttttttatca aagcgtttag
     1021 tcggcatacc agtgtcctgc atatctcttg tgcgtgataa cggagtgtaa caaaagggac
     1081 aggcatcaag ggattttttg ttattacagc cgtcgggtgg cgttgtacac ctgatcactg
     1141 tttctgcgtt ttatgaaaaa ataaaattat gtatcgatat ataaatatcg taaatagctg
     1201 gttataaata cgtctttaat tcatcagcac taaaactcat gctttggctg attacattta
     1261 tagcagcata atatgtttct acagttttat aatgattatg atgaatggct atatgaacag
     1321 ctgacatgtt gtcatgattt ttagcagcca acagtgtaaa taactgatgt tgactaaaag
     1381 aatgtttttt tgcaaaagca cccagcgtcg atatataaga taacacgacg tcttcattac
     1441 ccttgctcat agcgatgtat aatgcagggg ttccctgtgc tgtagcgcct tttaataaat
     1501 ccatgatgtt agctttgctc aacttgtatt taaaggcgat gccattaatt ttagagagga
     1561 accgtgtgac acacaaaggg tgattatttt ccattgcggc gtataatcct ggctcaccat
     1621 catgtatttt catggctaaa atactcatca ccagtttttt gtttaaaagc tcttgtgcta
     1681 taggagtggt taatgcattc agaaagatat cgattatctc atcgaatcca tacaacatgg
     1741 agatataaag tccaggtgtt tcgttagcac ttttagtttg caacaattta accagattat
     1801 cttcatgaat gatgtgatta tctaccagtg actgaatttc ttgcataaat attttcacgt
     1861 tgtcagtatg tccgttcatc atagccaaaa ataaagcagg tgtaccaatg ctattctgcg
     1921 cagttattat ttcaatgatt tctttttcag ggagtatggg caactgtctg aataatgtaa
     1981 taagctgttg agtctggcct gtggccatta ttaacaacat tgccgatgga tgtatagggg
     2041 caaatggcat tcgtgtcgcg atacccgtga aaatagcccc tggaactccc tcaggtaata
     2101 ggggcagaat agcaattgca cagttgcgaa catacctatc agcccaggtc tgagcatcgg
     2161 gacctgaact gatgaactta tcaagcgaga gaaagccccg tgctgtcctg tgggtgcctc
     2221 tgacggcaac gttggtatcg ttcgggtcgt aaacggacac aacataattt aattgcccat
     2281 acgccgactg ctttattcgt agccttactg acaaagtatg attgtctata tataaaatcg
     2341 cagacatatg actgacattg tttgccatca ttcgacagaa gaaaatatct aaagcgaagc
     2401 caaaatcctt actcgcgata acgcgactcc ctgcaggagg atggcgaagt aatgctctga
     2461 actccgcgtc ccggactaag gggacatgct gctgtatgga ttcaacggag gagtaatgtt
     2521 taatcaggtt gaccttgcct tttccatttc tcaatgaatc aagtgcgtat tgcaatgaca
     2581 gatggcgaca aaaaatagcg tcagaaggag attgcgaaaa aaatgcacta ccattgagat
     2641 ttactgttgg ttgtatagtt ttttctctat ggattttttt agacagtttc atacaatggt
     2701 tacgattttc ggcagttaac tgttcctgtt caatataatc cagtatgctt cgcaaatggt
     2761 tagctttaat aggcgtggtt tccagtaata aggaaatgat ttctttttta tagtgtaatg
     2821 taatgaatat attttctgca tggatgggtg aagtggcaaa gttaatggta aaaagggcag
     2881 tggaataaca attttgtgat atttttcgac agtttgcggt aaattcctga gtaacacagt
     2941 atgacagcat cgaacttaag tcagaggtat caagtagggt gatgagttgg ggcgcaaatt
     3001 gattaatagg ctctgatgaa ggcgttggta tgtcgctatc gatgtgcatt ctatgaggag
     3061 acttataatg agttctgata ataatgtcgt tttgactcat aatctattcg ccttatatat
     3121 gtctggaggt tttgagtatg atgattaatt attatcgcca gaacgaaatg tgcacgaaaa
     3181 attgctataa gaattgtgtc acttttatca ttgtgatatg accacttcgt cattaatctt
     3241 atgatcgttt aaatagtacc gccaaatcac gcaacgaact ttgctggatt cagtttatgc
     3301 tgctgtaaat tcgctcgcag taaacctttc agacgctccg tgatgttatc agttgttctt
     3361 taagcgtttt gctggtgtac tcactacaaa cgaattgcga atcgaatcaa tgtgaaacgg
     3421 aaaggtacaa tctccctttt tttagtgtta acatcgctca accgggtgga aggtgagagg
     3481 ttaatgtctt tcatttgtgt acctgattgc gaaccaattt agcatttgtt ggctagatgg
     3541 tttcgcaatg aactgttaat aaacaaattt ttctttgtat gtgatctttc gtgtgggtca
     3601 ccactgcaaa taaggatata aaatgcctgt tataactctt cctgatggca gtcaacgcca
     3661 ttacgaccac gctgtaagcc ccatggatgt tgcgctggac attggtccag gtctggcgaa
     3721 agcctgtatc gcagggcgcg ttaatggcga actggttgat gcttgcgatc tgattgaaaa
     3781 cgacgcacaa ctgtcgatca ttaccgccaa agacgaagaa ggtctggaga tcattcgtca
     3841 ctcctgtgcg cacctgttag ggcacgcgat taaacaactt tggccgcata ccaaaatggc
     3901 aatcggcccg gttattgaca acggttttta ttacgacgtt gatcttgacc gcacgttaac
     3961 ccaggaagat gtcgaagcac tcgagaagcg gatgcatgag cttgctgaga aaaactacga
     4021 cgtcattaag aagaaagtca gctggcacga agcgcgtgaa actttcgcca accgtgggga
     4081 gagctacaaa gtctccattc ttgacgaaaa catcgcccat gatgacaagc caggtctgta
     4141 cttccatgaa gaatatgtcg atatgtgccg cggtccgcac gtaccgaaca tgcgtttctg
     4201 ccatcatttc aaactaatga aaacggcagg ggcttactgg cgtggcgaca gcaacaacaa
     4261 aatgttgcaa cgtatttacg gtacggcgtg ggcagacaaa aaagcgctta acgcttacct
     4321 gcagcgcctg gaagaagccg cgaaacgcga ccaccgtaaa atcggtaaac agctcgacct
     4381 gtaccatatg caggaagaag cgccgggtat ggtattctgg cacaacgacg gctggaccat
     4441 cttccgtgaa ctggaagtgt ttgttcgttc taaactgaaa gagtaccagt atcaggaagt
     4501 taaaggtccg ttcatgatgg accgtgtcct gtgggaaaaa accggtcact gggacaacta
     4561 caaagatgca atgttcacca cgtcttctga gaaccgtgaa tactgcatta agccgatgaa
     4621 ctgcccgggt cacgtacaaa ttttcaacca ggggctgaag tcttatcgcg atctgccgct
     4681 gcgtatggcc gagtttggta gctgccaccg taacgagccg tcaggttctc tgcatggcct
     4741 gatgcgcgtg cgtggattca cccaggatga cgcgcatatc ttctgtactg aagaacaaat
     4801 tcgcgatgaa gttaacggat gtatccgttt agtctatgat atgtacagca cttttggctt
     4861 cgagaagatc gtcgtcaaac tctccactcg tcctgaaaag cgtattggca gcgacgaaat
     4921 gtgggatcgt gctgaggcgg acctggcggt tgcgctggaa gaaaacaaca tcccgtttga
     4981 atatcaactg ggtgaaggcg ctttctacgg tccgaaaatt gaatttaccc tgtatgactg
     5041 cctcgatcgt gcatggcagt gcggtacagt acagctggac ttctccttgc cgtctcgtct
     5101 gagcgcctcc tatgtgggcg aagacaacga gcgtaaggta ccggtaatga ttcaccgcgc
     5161 aattctgggg tcgatggaac gtttcatcgg tatcctgacc gaagaattcg ctggtttctt
     5221 cccgacctgg cttgcgccag ttcaggttgt tatcatgaat attaccgatt cacagtctga
     5281 atacgttaac gaattgacgc aaaaactatc aaatgcgggc attcgtgtta aagcagactt
     5341 gagaaatgag aagattggct ttaaaatccg cgagcacact ttgcgtcgcg tcccgtatat
     5401 gctggtctgt ggtgataaag aggtggaatc aggcaaaatt gccgttcgca cccgccgtgg
     5461 taaagacctg ggaagcatgg acgtaaatga agtgatcgag aagctgcaac aagagattcg
     5521 cagccgcagt cttaaacaat tggaggaata aggtattaaa ggcggaaaac gagttcaaac
     5581 ggcgcgccct aaccgtatca atggcgaaat tcgcgcccag gaagttcgct taacaggtct
     5641 ggaaggcgag cagcttggta ttgtgagtct gagagaagct ctggagaaag cagaagaagc
     5701 cggagtagac ttagtcgaga tcagccctaa cgccgagccg ccggtttgtc gtataatgga
     5761 ttacggcaaa ttcctctatg aaaagagcaa gtcttctaag gaacagaaga aaaagcaaaa
     5821 agttatccag gttaaggaaa ttaaattccg tcctggtaca gatgaaggcg actatcaggt
     5881 aaaactccgc agcctgattc gctttctcga agagggtgat aaagccaaaa tcacgctgcg
     5941 tttccgcggt cgtgagatgg cgcaccagca aatcggtatg gaagtgctta atcgcgtgaa
     6001 agacgatttg caagaactgg cagtggtcga atccttccca acgaagatcg aaggccgcca
     6061 gatgatcatg gtgctcgctc ctaagaagaa acagtaaggc cttcaagtag cactgtctgt
     6121 ggagccttcg ggcttcacag gctttgttcg cctttgtttc gtttattaac aatgcgaagt
     6181 ggaagttatt aaaatgccaa aaattaagac cgtacgcggt gctgctaagc gcttcaaaaa
     6241 aaccggtaaa ggtggtttta agcacaagca cgctaacctg cgtcacattc tgaccaaaaa
     6301 agcgaccaaa cgtaaacgtc acctgcgtcc gaaagccatg gtttccaaag gcgatctggg
     6361 cctggtaatc gcgtgcctgc cgtacgcata agccgttaac gtttttaact ttttaattag
     6421 aatatagata caggagagca catatggctc gcgtaaaacg tggtgttatt gcacgcgcac
     6481 gtcacaagaa aattttgaaa caagctaaag gctactacgg tgcgcgttct cgcgtatacc
     6541 gcgttgcctt ccaggctgtt atcaaagctg gtcagtatgc ttaccgtgac cgtcgtcaac
     6601 gtaagcgtca gttccgtcaa ctgtggattg cgcgtatcaa cgcagcagca cgtcagaacg
     6661 gtatttctta cagcaaattc atcaatggcc tgaaaaaagc ctctgttgaa atcgaccgta
     6721 agatcctggc tgatatcgca gtattcgaca aagtagcgtt caccgctctg gttgaaaaag
     6781 cgaaagcagc tctggcataa gccagttgaa agagggagct agtctccctc ttttcgtttc
     6841 aacgccatca aaacattgac ttttatcgcc gtagcctttt caataaaggt cttttgaaga
     6901 gtaccaaaag gtaacgcaag caatgaatgc tgctattttc cgcttctttt tttactttag
     6961 cacctgaatc caggaggcta gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct
     7021 cccagtggag gctttttttg tatgcgcgtt ttgaaaaata aaaagccagt ctatcaggcg
     7081 taattgtcgc agccagtctg gatacggcca gtgcggagaa accggagcgt acttaagtac
     7141 gtgaggattt cgagcactgc ccgggaccaa aatggcaagt aaaatagcct gatgggatag
     7201 gctctaagtc caacgaacca gtgtcaccac tgacacaatg aggaaaacca tgtcacatct
     7261 cgcagaactg gttgccagtg cgaaggcggc cattagccag gcgtcagatg ttgccgcgtt
     7321 agacaatgtg cgcgtcgaat atttgggtaa aaaagggcac ttaacccttc agatgacgac
     7381 cctgcgtgag ctgccgccag aagagcgtcc ggcagccggt gcggttatca acgaagcgaa
     7441 agagcaggtt cagcaggcgc tgaatgcgcg taaagcggaa ctggaaagcg ctgcactgaa
     7501 tgcgcgtctg gcggcggaaa cgattgatgt ctctctgcca ggtcgtcgca ttgaaaacgg
     7561 cggtctgcat ccggttaccc gtaccatcga ccgtatcgaa agtttcttcg gtgagcttgg
     7621 ctttaccgtg gcaaccgggc cggaaatcga agacgattat cataacttcg atgctctgaa
     7681 cattcctggt caccacccgg cgcgcgctga ccacgacact ttctggtttg acgctacccg
     7741 cctgctgcgt acccagacct ctggcgtaca gatccgcacc atgaaagccc agcagccacc
     7801 gattcgtatc atcgcgcctg gccgtgttta tcgtaacgac tacgaccaga ctcacacgcc
     7861 gatgttccat cagatggaag gtctgattgt tgataccaac atcagcttta ccaacctgaa
     7921 aggcacgctg cacgacttcc tgcgtaactt ctttgaggaa gatttgcaga ttcgcttccg
     7981 tccttcctac ttcccgttta ccgaaccttc tgcagaagtg gatgtcatgg gtaaaaacgg
     8041 taaatggctg gaagtactgg gctgcgggat ggtgcatccg aacgtgctgc gtaacgttgg
     8101 catcgacccg gaagtttact ctggtttcgc cttcgggatg gggatggagc gtctgactat
     8161 gttgcgttac ggcgtcaccg acctgcgttc attcttcgaa aacgatctgc gtttcctcaa
     8221 acagtttaaa taaggcagga atagattatg aaattcagtg aactgtggtt acgcgaatgg
     8281 gtgaacccgg cgattgatag cgatgcgctg gcgaatcaaa tcactatggc gggcctggaa
     8341 gttgacggtg tagaaccggt tgccggtagc ttccacggcg tggtcgttgg tgaagtggtt
     8401 gagtgtgcgc agcatccgaa cgctgacaaa ctgcgtgtga caaaagtgaa tgtcggcggc
     8461 gatcgcctgc tggacatcgt ctgcggtgcg ccaaactgcc gtcagggcct gcgtgtggcg
     8521 gtagcgacca ttggtgctgt tctgccgggt gatttcaaaa ttaaagcggc gaaactgcgc
     8581 ggcgaaccgt ctgaagggat gctgtgctcc ttttctgagc tgggaatttc tgacgaccat
     8641 aacggcatta tcgaactgcc tgcagatgcg ccgattggca ctgacatccg cgaatacctg
     8701 aaactcgatg acaacaccat cgaaatcagc gtaacgccaa accgtgccga ctgtttaggt
     8761 atcattggtg ttgcgcgtga cgttgccgtg ctgaatcagc tgccgctggt tgaaccggaa
     8821 atcgttccgg ttggtgcgac catcgacgac acgctgccga ttacagtcga agcgccggaa
     8881 gcctgcccgc gttatcttgg ccgtgtggta aaaggcatta acgttaaagc gccaactccg
     8941 ctgtggatga aagaaaaact gcgtcgttgc gggatccgtt ctatcgatgc agttgttgac
     9001 gtcaccaact atgtgctgct cgaattgggc cagccgatgc acgctttcga taaagatcgc
     9061 attgaaggcg gcattgtggt gcggatggcg aaagagggcg aaacgctggt gctgctcgac
     9121 ggtactgaag cgaagctgaa tgctgacact ctggtcatcg ccgaccacaa caaggcgctg
     9181 gcgatgggcg gcatcttcgg tggcgaacac tctggcgtga atgacgaaac acaaaacgtg
     9241 ctgctggaat gcgctttctt tagcccgctg tctatcaccg gtcgtgctcg tcgtcatggc
     9301 ctgcatactg atgcgtctca ccgttatgag cgtggcgttg atccggcact gcagtacaaa
     9361 gcgatggaac gtgcgacccg tctgctgatt gacatctgcg gtggtgaggc tggtccggta
     9421 attgatatca ccaacgaagc aacgctgccg aagcgtgcaa ccatcacttt acgtcgtagc
     9481 aaactggatc gcctgatcgg ccatcatatt gcggatgagc aggtaactga cattctgcgt
     9541 cgtctcggct gcgaagtgac cgaaggcaaa gacgagtggc aggcagttgc gccgagctgg
     9601 cgtttcgaca tggagattga agaagatctg gtcgaagaag tcgcgcgtgt ttacggctac
     9661 aacaacatcc cggatgagcc ggtacaggca agcctgatta tgggtactca ccgtgaagct
     9721 gacctgtcgc tcaagcgcgt gaaaacgctg ctcaacgata aaggctatca ggaagtgatc
     9781 acctatagct tcgttgatcc gaaagtgcag cagatgatcc atccaggcgt tgaagcctta
     9841 ctgctgccaa gcccgatctc tgttgaaatg tcagcaatgc gtctttctct gtggaccggc
     9901 ctgctggcaa ccgtggtgta caaccagaac cgtcagcaga accgtgtgcg cattttcgaa
     9961 agcggtctgc gtttcgtacc agatactcag gcaccgttgg gcattcgtca ggatctgatg
    10021 ttagccggtg tgatttgcgg taaccgttac gaagagcact ggaacctggc aaaagagacc
    10081 gttgatttct atgatttgaa aggcgatctt gaatccgttc tcgacctgac cggtaaactg
    10141 aatgaggttg agttccgtgc agaagcgaat ccggcactgc atccggggca atccgcagcg
    10201 atttatctga aaggtgaacg tattggtttt gttggggttg ttcatcctga actggaacgt
    10261 aaactggatc ttaacggtcg cactctggtg ttcgaactgg agtggaacaa gctcgcagac
    10321 cgcgtggtgc ctcaggcgcg cgagatttct cgcttcccgg cgaaccgtcg tgacatcgcg
    10381 gtggtggtcg cagaaaacgt tcccgcagcg gatattttat ccgaatgtaa gaaagttggc
    10441 gtaaatcagg tagttggcgt aaacttattt gacgtgtacc gcggtaaggg tgttgcggag
    10501 gggtataaga gcctcgccat aagcctgatc ctgcaagata ccagccgtac actcgaagaa
    10561 gaggagattg ccgctaccgt cgccaaatgt gtagaggcat taaaagagcg attccaggca
    10621 tcattgaggg attgaaccta tggcgcttac aaaagctgaa atgtcagaat atctgtttga
    10681 taagcttggg cttagcaagc gggatgccaa agaactggtt gaactgtttt tcgaagagat
    10741 ccgtcgcgct ctggaaaacg gcgaacaggt gaaactctct ggttttggta acttcgatct
    10801 gcgtgataag aatcaacgcc cgggacgtaa cccgaaaacg ggcgaggata ttcccattac
    10861 agcacggcgc gtggtgacct tcagacccgg gcagaagtta aaaagccggg tcgaaaacgc
    10921 ttcgcccaaa gacgagtaat ctgatctaac taaaaaggcc gctctgcggc cttttttctt
    10981 ttcactgtcg aagagtcacc gtaaaatcaa cgccatgaca cttcagcaga acggatacca
    11041 tgctgacact tgcccgccaa caacagcgac aaaatattcg ctggttatta tgcctgtcag
    11101 ttttgatgct gctggcgctt ctcttaagcc tttgcgccgg tgaacaatgg atctcgccag
    11161 gtgactggtt ttctcctcgt ggcgaactgt tcgtctggca aattcgcctg ccacgtacgc
    11221 tggctgtatt gctggttggt gcggcgctgg ctatatccgg cgctgtaatg caggcgttgt
    11281 ttgaaaatcc tctggcagaa cctggactac ttggcgtctc taacggcgca ggcgtggggc
    11341 ttatcgccgc ggtattgctt gggcaagggc aactccccaa ctgggcgcta gggctgtgtg
    11401 cgattgctgg ggcgcttatc atcactttaa tactcttacg tttcgcccgt cgtcatcttt
    11461 cgaccagtcg gttattgctg gctggcgttg cattagggat tatctgtagc gcactaatga
    11521 cgtgggctat ctacttttcc acctctgttg atttacgtca gctgatgtac tggatgatgg
    11581 gcggttttgg cggcgtagac tggcggcaaa gctggctgat gctggcattg atccccatgt
    11641 tgttgtggat ctgttgtcag tccaggccga tgaatatgtt agcacttggc gagatctcgg
    11701 cgcggcaact gggtttaccc ctgtggttct ggcgcaatgt gctggtggca gcgaccggct
    11761 ggatggttgg cgtcagtgta gcgctggcgg gtgctatcgg ctttattggt ctggtgatcc
    11821 cacatattct tcggttgtgt ggtttaaccg atcatcgcgc attacttccc ggctgcgcgc
    11881 tggcaggggc gagcgcattg ctgctggccg atattgtagc gcgcctggca ttagctgccg
    11941 cagagctgcc tattggcgtg gtcaccgcaa cgttgggtgc gccggtgttt atctggttat
    12001 tgttaaaagc aggacgttag ccgcaaaaag acggtctatg attaaaagct aagattttta
    12061 ctgaccacac ccaggagaaa cgatgcaaga ttccattctg acgaccgtag tgaaagatat
    12121 cgacggtgaa gtgaccacgc tggagaagta cgccggtaat gtgctgttga ttgtcaatgt
    12181 cgcctcaaag tgtggcttaa cgccgcaata tgagcagttg gagaatattc agaaagcctg
    12241 ggccgatcga ggttttgtgg tgctgggatt cccgtgcaac cagtttctgg aacaagaacc
    12301 gggcagcgat gaagagatta aaacttactg taccaccaca tggggggtga cgttcccgat
    12361 gttcagtaag attgaagtta atggcgaagg acgccatccg ctgtatcaaa aattgatttc
    12421 cgcagcgccg accgcagtcg cgccggaaga gagcggattc tatgcccgta tggtcagcaa
    12481 aggtcgtgca ccgctgtacc ctgatgatat tttatggaat tttgagaaat ttttagttgg
    12541 cagggacgga ttggtcatcc agcgtttttc cccggatatg acgccggaag atcccattgt
    12601 gatggaaagc attaaactgg cgttggcaaa ataatgtcta ttgtgatgca gttacaggat
    12661 gttgcggaat ctacccgcct ggggccgctt tctggcgagg ttcgggctgg ggagatcctg
    12721 cacctggtgg ggccgaatgg cgcgggtaag agtaccttac tggcgcgaat ggccggaatg
    12781 accagcggta agggaagcat tcagttcgcg gggcaaccac tggaagcatg gtccgcaaca
    12841 aaactcgcgc tgcatcgtgc ctatctttca caacagcaga cgccgccgtt tgcaatgccg
    12901 gtctggcact acctgacact gcatcagcac gataaaacgc gtaccgaact actgaatgat
    12961 gtcgcagggg cgctggctct tgatgacaaa ctcggtcgta gcaccaatca actttccggc
    13021 ggtgaatggc aacgcgtacg tcttgctgcg gtggtgctgc aaatcacacc acaagccaat
    13081 cccgcaggcc aattgctgct tcttgatgag ccgatgaaca gtcttgatgt tgcgcaacaa
    13141 agtgcgttag acaaaattct gagcgcgctg tgtcagcaag gactggcgat tgtgatgagc
    13201 agtcacgatc tcaaccacac attgcgtcat gcgcatcggg cgtggttgct aaaaggtgga
    13261 aaaatgctgg ccagtggacg cagagaagat gtgctcacgc cgccaaatct ggcgcaggcc
    13321 tatgggatga attttcgccg tctggatatc gaaggtcaca gaatgctgat ttcgaccatc
    13381 tgatttcatt agtatcttga aaatcgggta attacgacgc taaattaatg caagaataaa
    13441 aaacagagga accgtacgga atgcgtttct gccttatttt gatcacagca ctgtttctgg
    13501 ccgggtgtag ccaccataaa gcaccgccgc caaatgccag actttctgat tcgattaccg
    13561 ttattgccgg tttgaacgac cagctacaaa gctggcatgg cacgccgtat cgttatggtg
    13621 gcatgacgcg gcgcggtgtg gactgttcgg gatttgtggt tgtgacgatg cgcgatcgtt
    13681 tcgatttgca gctgccccga gaaaccaaag aacaagcctc tatcggcacg caaattgata
    13741 aagacgagtt gctgcctggt gacctggtct ttttcaaaac gggttccgga caaaatggtt
    13801 tgcatgtagg tatttacgat accaacaacc aatttatcca cgcctctacc agcaagggag
    13861 tgatgcgttc ctcacttgat aatgtctatt ggcagaaaaa tttctggcag gcgagacgaa
    13921 tctagtatgt cgaaaattgg acgtcgatga tacatcgccg tccatttact ccagaataca
    13981 gtagtatttt gcagacaaaa attccgtaaa tggattattc gtttataatc aggataatct
    14041 aaaaaaagga cccctgaaaa agtggttaac atggaacaaa tgaaagttac tccttctgta
    14101 attgttatgt cttatggcct catccctttt agccggatac tgaaaaacat ccttcgagag
    14161 ggacggttac catgaagatt tttttggaga atctttatca ttctgattgc tatttcctgc
    14221 cgataagaga taaccagcaa gtccttgtcg gcgttgagtt aattacccat ttctccagtg
    14281 aagatggcac ggtgcgtatc ccgacgagtc gggttatagc gcaacttacc gaggagcaac
    14341 attggcaatt attttcagag cagttggaat tactaaaatc atgccaacat ttttttattc
    14401 aacataaact ctttgcctgg cttaatttaa cgccacaagt ggctacatta ttattagaga
    14461 gggataatta cgcaggcgaa ctattaaaat atccgtttat tgaactgttg attaatgaga
    14521 attacccgca tctcaatgaa ggaaaagata atcgcggtct tttatcctta tcgcaagtgt
    14581 atccccttgt gttaggtaat ttgggagcag gcaacagcac aatgaaagcg gtttttgatg
    14641 gtcttttcac ccgcgtaatg ctggataaaa gttttattca gcaacaaatc acgcatcgct
    14701 ccttcgaacc gtttattcgt gcgattcagg cgcaaatttc cccttgttgc aattgcatca
    14761 tcgcgggggg cattgatacg ccagaaatat tagcacagat tacccccttc gatttccatg
    14821 ctctccaggg atgcctgtgg cctgccgttc ccataaatca gataacgacg ttggttcagc
    14881 gataaccctc ctgtttgctg gtgtttaaga cgagagtaac cgtctacact atcaaacagg
    14941 aggctctatg accctgtctt ttattacccg ctggcgcgat gaattgccag aaacctatac
    15001 agcactttcc cctacgcctt taaataatgc ccggctgatt tggcataata ccgaactcgc
    15061 taacacgctg agtattccat cgtcgctgtt taaaaatggc gcaggtgtct ggggcggcga
    15121 aaccctactg cctggtatgt caccactggc gcaggtttac agtggtcatc agttcggcgt
    15181 ctgggcgggc caactgggtg atgggcgcgg cattttactt ggcgaacaac tgcttgctga
    15241 tggcactaca atggactggc atctgaaagg tgctggcctg acgccttatt cgcgaatggg
    15301 tgatggacgg gcggtattac gttcgacgat acgagaaagt ctcgccagtg aggcgatgca
    15361 ttatctgggc attccgacga cccgcgcgtt aagtatcgtc accagcgatt cgccagtgta
    15421 tcgggaaacg gtggagccag gcgcgatgct gatgcgtgtg gcaccaagtc atctgcgctt
    15481 tggtcatttc gaacattttt actatcgccg cgagccggat aaggttcgtc agttggctga
    15541 ctttgccatt cgtcattact ggtcacatct tgaagatgat gaggacaaat accgtctctg
    15601 gtttaacgat gttgtcgcac gtaccgcatc gttaattgcc caatggcaga cggtcggctt
    15661 tgcacatggg gtgatgaata ccgacaacat gtcgctgctg ggactgacgc ttgattacgg
    15721 gccgtttggt tttcttaatg attacgaacc cggttttatt tgtaatcact cggatcatca
    15781 aggacgttac agctttgaca atcaacctgc tgtcgcgttg tggattttac agcgtctggc
    15841 gcagacattg tcaccatttg ttgccgtaga tgccctgaat gaggccctgg acagctatca
    15901 gcaggttttg ttgacgcatt atggacaacg gatgcggcag aaactgggct ttatgacgga
    15961 gcaaaaagag gataacgcgc tactgaatga attattcagt ctgatggcgc gagagcgcag
    16021 cgattatacc cgcacatttc gcatgctgag tctgaccgag cagcacagcg cggcgtcacc
    16081 gctacgtgat gagtttattg atcgtgcggc atttgatgac tggtttgccc gttatcgagg
    16141 gcgtttgcaa caagacgagg ttagcgatag tgagcgtcag caactgatgc aaagcgttaa
    16201 ccccgctctg gtattgcgca actggttggc gcaacgggcg attgaagcgg cagaaaaggg
    16261 tgatatgacg gaattgcacc gcctgcatga ggcgttgcga aatcctttca gcgacagaga
    16321 tgatgactat gtcagtcgtc cacctgactg gggtaaacgg ctggaagtca gctgctcgag
    16381 ttaaaggcta cttggtcaac agcagcttgc cagcctgagt tttacggagc agatattctt
    16441 gcccgtcatg atcgataatc agtttgccat ccggacctaa cagtgtctgg ctggaaattc
    16501 gccgaatagg ctgcggggaa gcggtcttgt gattggcatc cgtttcaggc gtgagttttc
    16561 tgctatccgt ataacgcata aaaattaata ataattgata taacaatgat tcttattatc
    16621 aatattatgg ctgaccagag caagcagtaa tgtgttgaga agacaacaag ttgcgcagaa
    16681 atgcccatcc catcaggaat gggcacgcat tcagaagcgg gtatctaccg cagaggctag
    16741 tttttcgacc aggcgttcgg tatcttccca gcccagacac gggtcggtaa tggattgacc
    16801 gtaagtgagc ggctgaccgc cgacgatttt ttgcgttcct tcgcgcagga aactttccgc
    16861 cataattcca gcaatcgccg tagagccatt gcggatttgc tgacaaatat cctcacaaac
    16921 ttctaactgg cgacggtgct gcttctggca gttaccgtgg ctgaaatcca ccaccagatg
    16981 ttcaggtaaa tcaaactcgt gcagcgtatc gcaggctgcg gcgatatcat cggcatgata
    17041 attcggtttt ttgccgccac gcataataat gtggccatac gggttgccgc tggtctgata
    17101 gatggtcatc tgaccatttt tgtctggcga gaggaacata tggctggcgc gggctgcgcg
    17161 gatagcatcc acagcaatcc gcgtattgcc atcggtacca tttttaaaac ctaccggaca
    17221 ggagagtgcc gaagccattt cacggtggat ctgactttcg gtagtacgtg cgccaatcgc
    17281 gccccaactg attaaatcag caataaactg accggtcacc atatcgagga actcagtcgc
    17341 ggttgggacg cccagctcat ttacctgtaa aagtaatttg cgcgccagct ccagaccgtg
    17401 atttatccga tagctgccgt ttaaatctgg atcggagatt agtcctttcc agccgacaac
    17461 agttcgtggt ttttcaaaat aggtgcgcat tacgatttcc agccgtgact ggtactggtt
    17521 gcgcagcgac tgcagacggg tggcgtactc cattgcagcg gtgagatcgt ggatcgagca
    17581 ggggccaata atgaccaaca gtcgcttatc ttcaccattg agtatttttt caattctgcg
    17641 gcgggagtcg gtgacatggg tggcgacgcc aggcgttacg ggataccgta gcgcgagttc
    17701 ggcgggcgtt accaggctct caatacgcgc agtacggagt tcgtcagttc tgttcattac
    17761 aagtctcaga aatttgttgc ttctacggca ggactgccgg gaagtgatgc gcatcacgat
    17821 aaaccaatcg ctgctgattt caagtgcgag gtgtgtcaca cctcgccggg tggttagcat
    17881 gataacaaaa aaataagcta atgcactagt tctctagtac attcggcgac taaggcccat
    17941 gatatcgagg atcttggtgg caatctcttc taccgaataa ttggtactgt taatccacgg
    18001 gatctgattt ttacggtaca aggcttccac ttccgcgact tccatcctgc actgacgaag
    18061 cgaggcatag cgactgttct cccgacgttc ctcgcgaatc gccgccagac gttccgggtc
    18121 gatagtcagg ccgaacaatt tatgctgaag cggtttgagc gacgcgggta gcaccagatt
    18181 atccatatcg tcggcaataa aggggtagtt tgccgcgcgg ataccaaatt gcattgccag
    18241 atacagactg gtgggggttt taccacagcg agaaacaccg agcaggatca cctgagcctg
    18301 atccagattg cgcaacgaaa tgccgtcatc gtgggcgagg gtgtaatcaa tcgccgcaat
    18361 gcgcgcatca tatttattga gattattagg gttaaggcca tgggtacgat gagcaatcgg
    18421 cgttggatcc agtttcatct cttgttgtag cggggcaacc agcgcctgaa cgatatcctg
    18481 gcaaaaacct tcactttgca agatgatggc gcgaatttcc ggcaacacga tggagtagaa
    18541 gaccagcgga cgcacgcctg tctggtgata aattgcgtca atctgatcct tcactgcccg
    18601 tgcacggctc tcattttcga caaacggcag cgtgatgctg ctgatagtga cgggaaattg
    18661 tgacattact gcgtgtccta atacctccgc agttattgcc gtaccatcag aaatataaaa
    18721 aacgtggcga tcaacagcat tatccatttt gttcttcccg tgatgcagac ataattctat
    18781 gaaagcataa attaaaaacg cacagaagcg tagaacgtta tgtctggttt ataaaatgaa
    18841 ccttcaattt tattttttat gaaaacagca tttcattttt atggtttcgt ttataccgat
    18901 ggtttatgtg gaaattgtcg aaaagagcag atttgcgcaa cgctgggatc agtcttaaca
    18961 agtaaaaaaa tatatttgct tgaacgattc accgtttttt tcatccggtt aaatatgcaa
    19021 agataaatgc gcagaaatgt gtttctcaaa ccgttcattt atcacaaaag gattgttcga
    19081 tgtccaacaa tggctcgtca ccgctggtgc tttggtataa ccaactcggc atgaaggatg
    19141 tagacagggt tgggggcaaa aatgcctccc tgggtgaaat gattactaac ctttccggaa
    19201 tgggtgtttc cgttccgaat ggtttcgcca caaccgccga cgcgtttaac cagtttctgg
    19261 accaaagcgg cgtaaaccag cgcatttatg aactgctgga taaaacggat attgacgatg
    19321 ttactcagct tgcgaaagcg ggcgcgcaaa tccgccagtg gattatcgac actcccttcc
    19381 agcctgagct ggaaaacgcc atccgcgaag cctatgcaca gctttccgcc gatgacgaaa
    19441 acgcctcttt tgcggtgcgc tcctccgcca ccgcagaaga tatgccggac gcttcttttg
    19501 ccggtcagca ggaaaccttc ctcaacgttc agggttttga cgccgttctc gtggcagtga
    19561 aacatgtatt tgcttctctg tttaacgatc gcgccatctc ttatcgtgtg caccagggtt
    19621 acgatcaccg tggtgtggcg ctctccgccg gtgttcaacg gatggtgcgc tctgacctcg
    19681 catcttctgg cgtgatgttc tccattgata ccgaatccgg ctttgaccag gtggtgttta
    19741 tcacttccgc atggggcctt ggtgagatgg tcgtgcaggg tgcggttaac ccggatgagt
    19801 tttacgtgca taaaccgaca ctggcggcga atcgcccggc tatcgtgcgc cgcaccatgg
    19861 ggtcgaaaaa aatccgcatg gtttacgcgc cgacccagga gcatggcaag caggttaaaa
    19921 tcgaagacgt accgcaggaa cagcgtgaca tcttctcgct gaccaacgaa gaagtgcagg
    19981 aactggcaaa acaggccgta caaattgaga aacactacgg tcgcccgatg gatattgagt
    20041 gggcgaaaga tggccacact ggtaaactgt tcattgtgca ggcgcgtccg gaaaccgtgc
    20101 gctcacgcgg tcaggtcatg gagcgttata cgctgcattc acagggtaag attatcgccg
    20161 aaggccgtgc tatcggtcat cgcatcggtg cgggtccggt gaaagtcatc catgatatca
    20221 gcgaaatgaa ccgcatcgaa cctggtgacg tgctggtcac tgacatgacc gacccggact
    20281 gggaaccgat catgaagaaa gcatctgcca tcgtcaccaa ccgtggcggt cgtacctgtc
    20341 acgcggcgat catcgctcgt gaactgggca ttccggcggt agtgggctgt ggtgatgcaa
    20401 cagaacggat gaaagacggt gagaacgtca ctgtttcttg tgccgaaggt gataccggtt
    20461 acgtctatgc ggagttgctg gaatttagcg tgaaaagctc cagcgtagaa acgatgccgg
    20521 atctgccgtt gaaagtgatg atgaacgtcg gtaacccgga ccgtgctttc gacttcgcct
    20581 gcctgccgaa cgaaggcgtg ggccttgcac gtctggaatt tatcatcaac cgtatgattg
    20641 gcgtccaccc acgcgcactg cttgagtttg acgatcagga accgcagttg caaaacgaaa
    20701 tccgcgagat gatgaaaggt tttgattctc cgcgtgaatt ttacgttggt cgtctgactg
    20761 aagggatcgc gacgctgggt gccgcgtttt atccgaagcg cgtcattgtc cgtctctctg
    20821 attttaaatc gaacgaatat gccaacctgg tcggtggtga gcgttatgag ccagatgaag
    20881 agaacccgat gctcggcttc cgtggcgcgg gacgctatgt ttccgacagc ttccgcgact
    20941 gtttcgcgct ggagtgtgaa gcagtgaaac gtgtgcgcaa cgacatgggg ctgaccaacg
    21001 ttgagatcat gatcccgttc gtgcgtaccg tagatcaggc gaaagcggtg gttgaggaac
    21061 tggcgcgtca ggggctgaag cgtggtgaga acgggctgaa aatcatcatg atgtgtgaaa
    21121 tcccgtccaa cgccttgctg gccgagcagt tcctcgaata tttcgacggc ttctcaattg
    21181 gctcaaacga catgacgcag ctggcgctcg gtctggatcg tgactccggc gtggtgtctg
    21241 aactgttcga tgagcgcaac gatgcggtga aagcactgct gtcgatggcg attcgtgccg
    21301 cgaagaaaca gggcaaatat gtcgggattt gcggtcaggg tccgtccgac cacgaagact
    21361 ttgccgcatg gttgatggaa gaggggatcg atagcctgtc tctgaacccg gacaccgtgg
    21421 tgcaaacctg gttaagcctg gctgaactga agaaataaaa taaatccccg gcggcgtttt
    21481 gtcgccgggt ttatgtgatc cccgaagatg aaacttattc aatctcttca cagacatcct
    21541 gcgttaaccg ccgcataata tctttccgta acaaaaactt ttgtatttta cctgaggcag
    21601 tgcgcggtag tttttcgatt accacgatat gttcaggata tttatatttt gcgacccgtt
    21661 tacggctaaa aaaagccacc acctcttcca gcgataatga atgatgcggc gctttcagca
    21721 cgacataagc gcatgatcgt tcacctaaac gttcatcggg cattgcaacc acacaggcat
    21781 cgtgaatttt aggatgctgc aataaaatat cttccacttc acggctgcta atattttcgc
    21841 cgccgcggac aataatatct tttttgcgtc cggtaatttt tatatagcca gcctcatcca
    21901 tacggcagag atcgccgctg taataccagc cttcttcatc cagggcacgg gcggttaatt
    21961 caggttcatc aaaatatccc ataaacacat tggggccacg cgaggcttct tcaccttcgc
    22021 aacctggcgg taaggtcttg cgtgcgtcat cgaccacttt aatctctaca cctgcggcag
    22081 cgtaaccatc ggtgtgcata aagcgcgaca aaggatcatc gagattcacc accgcatgcg
    22141 gcgaactttc tgtggaacca taaacactta ataatttaat gccgcgctgc tggcattcac
    22201 gcgccacttt tttggggatt gtggtaccgc cgcaaagaaa gaagcgcagc gctgaaaggt
    22261 ctgcgggttg tttctctagt aaattcaaaa gatcatagac aaacggcgtt gcgccgagca
    22321 tacaggagca acgctgctgc tcaagcagcg cgagacacgc atcaggagtg aaaatatcta
    22381 acaacacgct gcgagcgcca attaagaatg gtgccgttac gccatgcaga aagcccgttg
    22441 cgtgaccaag tggcgcaggc atcataaaga catcctgcca ggttagattc agccgcgcgc
    22501 aataagcccg ctcactggcg agaatattgt tatgcgtaag catcacgccc tttggcagac
    22561 cctcggttcc ggaggtaaac agcaccgcag ctaattcatc gccgtgggtc gttatcgccg
    22621 tggtcagtgg ggtattgtcg gcgataatct gacttaatga gagggaagag gtggcgggag
    22681 ccagtttgtc cacgccgaca atttgttgta gttgtggaag ctgattttgc agcggcagga
    22741 ttaaatctac cggacgcgtt tgtttaaaca acgtcggtgt aaagaacatt tttgcctgac
    22801 acttattaag cacccatacc agttctgctt cccgccagga aggcaacagc ggtacggaaa
    22861 ccgcaccgat tttcaggcag gcaagataga taacggtaaa ttcacaccag cctggcagtt
    22921 gaaatgctat gcgatcgcct gattcaatac ccttcgctaa catccagttt gccagacagc
    22981 tcgcggcgtg atcgagcgcg ctataggtgt acgatgctcc atgattatcg accacggcaa
    23041 ttttgtctgg catcgcacga gcggtctgct gccagtaatc ggccagcgaa gcatcgcccc
    23101 atagcccttg ctgacgatac gccgcacgac gttgttcgtt aaacgttaat gtcactttca
    23161 ttttggactc tcgaaacaga acatcaggcc cgagatgcgg gcctgtggga tgcatgatta
    23221 gccgtagcga aaatcaatac cgaaggtgcc tgcgggatat tgccactgtt cgagaatagt
    23281 gttaccgcac agcacccgac aggtgccgca ctccagacaa ccggcggaat caaaatgaat
    23341 gtttcctgcg tcatcctgct tataaagtcc ggcagggcag gctttcatta atttacggaa
    23401 ttcattgata tcgggatttg ccgccaaaat gatatgcggg tggccctcat caacatggaa
    23461 tttattgacg cctaatttga tgtcgacgtt aaccgtagcg ttctggctca tagcgcggtt
    23521 gctcccttaa tgccatcttt cagcaagttg atcagcccaa ttttcttcgc gtgtcccatg
    23581 atcattttgc gtaccggctg gttaggctta ccgtcaatgg tgaacatctc gttcatgatg
    23641 tcggctacca ttcgtgggta ttggctaaac aggcgcgggt tttccatcag cgccgggatc
    23701 ttgcgaaaat gctgcatatc acgcatgacg cagctttgtt ccagctcacg tttgtattgc
    23761 gccagactgc tggcggagaa atccgcgcgt tctttggcgg cgatcactgt tgtggcggca
    23821 gcctgagccg atgcaatggc taaatccatg ccgcggaccg taaaacccaa attcaggcag
    23881 aagcctgcgg cgtcaccaac gatcatcacg ccatcgttaa ccagttgcgg taccatcgcc
    23941 agaccgcctt ctggcaccat atgcgcggaa tattcaagca gtttgccgcc gctaatcagc
    24001 gggcgaatgg cggggtgttg tttaaaatct tccagcattt gcggcacgct tttttgcgca
    24061 tgggcgatat cagccaatcc acaaaccagc cccaaggata tggaatcctt gttggtatag
    24121 agaaatcccc cgcccatcag gccgtcagaa ggggaaccgg caaacagcca ggcggcacct
    24181 tcctcgcccg taatattaaa gcgatcgttg atctgttctg gtgtgaggcc aataacctct
    24241 ttaacaccaa cagcgtaatg atgcggatcg gaagcgggaa ccattcccag cgagcggcca
    24301 agcatcgagt taacgccatc ggctaaaatc accacattgg cttcgagaat atcatccccg
    24361 gcctggactc cagtgacctt gtttccttca cgaaccaacg catcgacgcg aactcccggg
    24421 ataaactgtg cgcccgcctg ctcggcttgt tccatcaacc acgggtccag acgattacgc
    24481 aatacggtat aagatgcgtg ttgcggaaca tctggttgct cgcggtgaaa atcgagggta
    24541 acggcgctct cttcggttaa gaaggagatt ttctcgcgtg tgaccttgcg ttctaccggc
    24601 gctgatgctg caaagcctgg aatgattgct tcaagtgtgt gggcataaag acgcccgccg
    24661 gtcatgtttt tacatccggc actgtcgccg cgttctatca ccaggatatc cagcccggct
    24721 cgcgccatga ccagtgcggc aacgctacca gcaacgcccg caccgaccac aatggcatca
    24781 aatttgtcat ccgacatgcg ttatgctccc ttcccgctgc gtttacagcg ggagatgtgg
    24841 ttgaaagggg aattagcggc tcaactggct aatcagggca gggacgactt tgtagatatc
    24901 gcccaccaga ccgtagtcgg catagttgaa gattggcgca tttttatctt tattgatggc
    24961 gacaatcact tttgcgccgt tgccgccaac catatgctgg atctgcccgg agatccctag
    25021 cgtcaggtag agatcggatt tcagcaacac gccggagaca ccgatataac gttcacgctc
    25081 catccagttc tcgccttcgg caattggacg tgaacagccg acttcagcat tcagcaccgc
    25141 cgccagttcg tggaccattt tcaggtcatc ctgcgctgcc agaccacgac cgacgccaac
    25201 cacacgtttt gctttgctca ggtccacgct gcttgcggct ttcgcacggc gttcgcgaca
    25261 gagaatttca tgacgcggag caacgtaagg taccgtttct gtcgggcact gatgagaggt
    25321 atcactggtg cacggttcct gaacaccggg tgcaagggta atgatcgcca gcgagctgtt
    25381 gatcttttcc tgagcgaacg ctaacccgcc atacatccgg tgttcggcgc aaatgtgacc
    25441 atcgacaata tccaccgccg tggcatcgtt caccagcgcc gcattcagtt gcacacttaa
    25501 ccgtgctgcc agcgctttac cacgtttcgt cgcggccagt agcaacatag cggggtgttt
    25561 atctttcagc agagcagcaa tgctttcggc gtaattttca gtgcgttgca gcgcgtcgtt
    25621 ttgctcaaga acataaatac attttggacc ataaggcata actgcctgcg cctggtcggt
    25681 attttgtaca atggcataca cctgttggcc ccattgctga gcgccgccaa acagttcagc
    25741 ataacgttca ggattatcgc taaagaccca gacgctgttt aattgactca tcgtttcttc
    25801 tccgtaggct tcagttcagg gctttcttca gatgttccgc cagctcggca atggcctccg
    25861 gcgaatcgtt atcgagaatg atgtgcttac gttctgtttg cggcggtacg cgaatgccgg
    25921 tcagttcagc aagtggtgcg ctctggctcc agccaatatc acttgcctgc cactgattta
    25981 ccggtttttt acccgcgccg agaatggctt tcatcgaagg aatgcgtggc acgttaatat
    26041 cggaggtgac gcagagcacg gctggaacag agagttcaat aacttcaacg tcatcttcaa
    26101 gcgtgcgttc aatcaccagt gtattgccct gacgctgaat agcactcact gcattaatca
    26161 ccggaagttg cagaatttct ccgaccagca agccaacctg ctgggcataa aggtcgccgg
    26221 aacctttacc aaagatcagt aaatcgaagc cgatcttttc aactgctgcc gccagcgctt
    26281 ttgcggtatc gagaggcagt gcatgctcaa gttgtgcatc ctgcaccata tacaggctgt
    26341 gcggcccgcg ggatagcacg tctttgcgca ctttcgagtt ctgcaacaat gagccgccaa
    26401 cggtcagcgc ggctatctca tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat
    26461 tgagatcgaa ctggctgatt ttggcgtcgg cattgtcgaa attcagggtg tattctggag
    26521 tgacaacaat gtcctgttct tcaggcacca gcttaaagca ggttattatt ttcatgattt
    26581 ctcctgtgag tcgcatacgg aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt
    26641 gtgcgatagc ggtgcggggt tttcgacgga gcaagctgtt ttccttgcca acaatcttgc
    26701 aaaaaaaatg cgatcgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac
    26761 cgtacaatct tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat
    26821 aaccgcaata cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag
    26881 ggaggttgtc atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgctgg
    26941 taaaacgcca cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtctggaca
    27001 tcacgttcat gacaatgaaa ccgagctgat ttacgtcaag aaaggggtgg caaggtttac
    27061 catcgattct tcgttatatg tcgcgcatgc agatgatata gtggtgatag aacgcggcag
    27121 gctgcatgcg gtggcctctg acgttaacga tccggcaacg acgtgtactt gtgcgctgta
    27181 cggctttcag tttcaggggg ctgaggaaaa tcatctactg caaccgcatt cttgtccggt
    27241 aattgccgcg gggcagggaa aagaagtcat taaaacctta tttaatgagc taagtgtgat
    27301 tttgccgcaa agtaaaaata gccaaacatc ttcgttatgg gatgcatttg cctatacatt
    27361 agcaattctt tactacgaaa actttaaaaa tgcttatcgt tcggagcagg ggtatat
//
LOCUS       DAZKIF010000059        27082 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZKIF010000059 DAZKIF010000000
VERSION     DAZKIF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.059"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(163..1827)
                     /gene="glnS"
                     /locus_tag="ACHOTW_002459"
     CDS             complement(163..1827)
                     /gene="glnS"
                     /locus_tag="ACHOTW_002459"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP9844556.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2030..3976)
                     /gene="nagE"
                     /locus_tag="ACHOTW_002460"
     CDS             complement(2030..3976)
                     /gene="nagE"
                     /locus_tag="ACHOTW_002460"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP9844557.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4309..5109
                     /gene="nagB"
                     /locus_tag="ACHOTW_002461"
     CDS             4309..5109
                     /gene="nagB"
                     /locus_tag="ACHOTW_002461"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9844558.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5169..6317
                     /gene="nagA"
                     /locus_tag="ACHOTW_002462"
     CDS             5169..6317
                     /gene="nagA"
                     /locus_tag="ACHOTW_002462"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9844559.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6326..7546
                     /gene="nagC"
                     /locus_tag="ACHOTW_002463"
                     /gene_synonym="nagR"
     CDS             6326..7546
                     /gene="nagC"
                     /locus_tag="ACHOTW_002463"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP9844560.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7594..8346
                     /gene="nagD"
                     /locus_tag="ACHOTW_002464"
     CDS             7594..8346
                     /gene="nagD"
                     /locus_tag="ACHOTW_002464"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP9844561.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8603..10267
                     /gene="asnB"
                     /locus_tag="ACHOTW_002465"
     CDS             8603..10267
                     /gene="asnB"
                     /locus_tag="ACHOTW_002465"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP9844562.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10647..10723
                     /locus_tag="ACHOTW_002466"
     tRNA            10647..10723
                     /locus_tag="ACHOTW_002466"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10681..10683,aa:Met,seq:cat)
     gene            10734..10818
                     /locus_tag="ACHOTW_002467"
     tRNA            10734..10818
                     /locus_tag="ACHOTW_002467"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10768..10770,aa:Leu,seq:tag)
     gene            10842..10916
                     /locus_tag="ACHOTW_002468"
     tRNA            10842..10916
                     /locus_tag="ACHOTW_002468"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10874..10876,aa:Gln,seq:ttg)
     gene            10951..11025
                     /locus_tag="ACHOTW_002469"
     tRNA            10951..11025
                     /locus_tag="ACHOTW_002469"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10983..10985,aa:Gln,seq:ttg)
     gene            11041..11117
                     /locus_tag="ACHOTW_002470"
     tRNA            11041..11117
                     /locus_tag="ACHOTW_002470"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11075..11077,aa:Met,seq:cat)
     gene            11166..11240
                     /locus_tag="ACHOTW_002471"
     tRNA            11166..11240
                     /locus_tag="ACHOTW_002471"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11198..11200,aa:Gln,seq:ctg)
     gene            11278..11352
                     /locus_tag="ACHOTW_002472"
     tRNA            11278..11352
                     /locus_tag="ACHOTW_002472"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11310..11312,aa:Gln,seq:ctg)
     gene            complement(11506..12681)
                     /gene="ubiF"
                     /locus_tag="ACHOTW_002473"
     CDS             complement(11506..12681)
                     /gene="ubiF"
                     /locus_tag="ACHOTW_002473"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP9844563.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12827..14251
                     /gene="miaB"
                     /locus_tag="ACHOTW_002474"
     CDS             12827..14251
                     /gene="miaB"
                     /locus_tag="ACHOTW_002474"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP9844564.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14404..15444
                     /locus_tag="ACHOTW_002475"
     CDS             14404..15444
                     /locus_tag="ACHOTW_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9844565.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15441..15908
                     /gene="ybeY"
                     /locus_tag="ACHOTW_002476"
     CDS             15441..15908
                     /gene="ybeY"
                     /locus_tag="ACHOTW_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9844566.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15998..16876
                     /gene="corC"
                     /locus_tag="ACHOTW_002477"
     CDS             15998..16876
                     /gene="corC"
                     /locus_tag="ACHOTW_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP9844567.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16901..18439
                     /gene="lnt"
                     /locus_tag="ACHOTW_002478"
     CDS             16901..18439
                     /gene="lnt"
                     /locus_tag="ACHOTW_002478"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP9844568.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18836..19744
                     /gene="gltI"
                     /locus_tag="ACHOTW_002479"
     CDS             18836..19744
                     /gene="gltI"
                     /locus_tag="ACHOTW_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP9844569.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19914..20654
                     /gene="gltJ"
                     /locus_tag="ACHOTW_002480"
     CDS             19914..20654
                     /gene="gltJ"
                     /locus_tag="ACHOTW_002480"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP9844570.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20654..21328
                     /gene="gltK"
                     /locus_tag="ACHOTW_002481"
     CDS             20654..21328
                     /gene="gltK"
                     /locus_tag="ACHOTW_002481"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP9844571.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21328..22053
                     /gene="gltL"
                     /locus_tag="ACHOTW_002482"
     CDS             21328..22053
                     /gene="gltL"
                     /locus_tag="ACHOTW_002482"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP9844572.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22171..23106
                     /gene="rihA"
                     /locus_tag="ACHOTW_002483"
     CDS             22171..23106
                     /gene="rihA"
                     /locus_tag="ACHOTW_002483"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP9844573.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23190..24860
                     /gene="hscC"
                     /locus_tag="ACHOTW_002484"
     CDS             23190..24860
                     /gene="hscC"
                     /locus_tag="ACHOTW_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFP9844574.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
     gene            complement(24920..26371)
                     /gene="djlC"
                     /locus_tag="ACHOTW_002485"
                     /pseudo
     CDS             complement(24920..26371)
                     /gene="djlC"
                     /locus_tag="ACHOTW_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(26368..27075)
                     /locus_tag="ACHOTW_002486"
     CDS             complement(26368..27075)
                     /locus_tag="ACHOTW_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9844575.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         6645 a         7034 c         6880 g         6523 t
ORIGIN      
        1 aacgccttat ccggcctaca aaagcacgca aactcaatat attgcagaga tcatgtaggc
       61 ctgataagcg tagcgcatca ggcaatttag cgtttgaaac tgtttgtctg ttccttgccg
      121 gatgcgacgt aaacgcccca tccggcatag cgaaacttaa aattactcgc ctactttcgc
      181 ccaggtatca cgcagcccaa cggtgcggtt aaataccggt ttttccgccg tagaatggcg
      241 gctatcgagg cagaagtaac cttcacgctc aaactggaat gctttacccg caaccgcatc
      301 tttcagcgac ggttcagcaa agccctgttt gatcaccagc gattccgggt taatcaccga
      361 caggaaatca tccgcagcac ctgggttcgg cacgctgaac agacggtcat acaaacggat
      421 ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata acacctttga ctttacgacc
      481 atctgccgga tctttgctta aggtatcggc gtcataagta cagaagatgg tggtgatatt
      541 accttcggca tctttctcga cgcgttctgc cttaatcaca taagcattac gcagacgcac
      601 ttctttaccc agcaccagac gtttgtactg cttgttagct tcttcgcgga aatcggagcg
      661 atcaatccaa atctcaccgc taaacggcac ctgacggctc cccatttccg gtttgttcgg
      721 atggttcggc atggtaacca tttcgccttc gccctgatag ttttcgataa ccagtttcac
      781 cggatcgata accgccattg cgcgcggcgc attttcgttg agatcttcac ggatgcagga
      841 ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc acgccgatgc gtttgcagaa
      901 ctcacgaata gaagccgcag tgtaaccacg acgacgcaga ccggaaatgg tcggcatacg
      961 cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc aagttcagct tacgcttgga
     1021 catcacagtg tattccagat tcaggcgcga gaattcgtac tggcgcgggt gaacaggaat
     1081 ggtgatgttg tccagtaccc agtcgtacag acgacggtta tcctggaact caagcgtaca
     1141 cagagagtgc gtaatacctt ccagggcatc gctgatgcag tgggtgaagt cgtacatcgg
     1201 gtagatgcac cacttgttgc cagtctggtg gtgttcagca aatttaatgc ggtacagcac
     1261 cggatcgcgc atcacgataa acggtgaagc catgtcgatt ttcgcacgca ggcaggcttt
     1321 accttcttca aaaccgccgg tacgcatttt ctcgaacagc gccaggttct cttcaacgct
     1381 acggtcgcgg tacgggctgt ttttaccagg ctgcgtcagg gtgccgcggt attcgcggat
     1441 ctgttccggc gtcagttcat caacgtacgc caggccttta ttgatcagtt cgatcgcata
     1501 ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc agtgaaaacc
     1561 taaccactca acgtcgtttt tgatcgaatc gacatactcg atatcttctt ttaccgggtt
     1621 agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc cgaagttcag
     1681 gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg ggaaacgggt
     1741 gtgtactgtg gtgtgcttac cactggccag atcttcatcg atgatctgac ggataaagtt
     1801 agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac aacgtataac
     1861 ggcgtatgat cttataagcg ggacaggctg acaactgtta gttagtgaaa atcgaaaatc
     1921 tcactgagat tatggggatg attgatgaaa aaaaaagcgg cagagaagat ctccaccgct
     1981 tgtcattgtt ggatgcgacg ctcaagcgtc gcatcaggca taaagcagat tactttttga
     2041 tttcatacag cggtgtttga cccgccacaa catggccctg agctttaatg atcaagccac
     2101 tgaaatcgtc gatattgctg caaaccaccg ggctaatcat cgaacgggcg ttagcgttca
     2161 ggtaatccag atccatttcc agaatcggtt gccctgcgct tacctgcgcg ccctcttcca
     2221 ccagacgttt aaagccttta ccttccagcg ctacggtatc gatacccatg tgaacgacaa
     2281 tctccgcgcc tttttcggtt tccaggcaga acgcgtggtt ggtgttgaag attttcacga
     2341 ttgttcctgc ggctggtgat acgacgattt tatctgtcgg tttcaccgcc acaccgtcac
     2401 ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc cagtgccacg acatcaccgg
     2461 taatcggcga taccagctcc gcgatagata ccgcgtttgg tacagcctgc ggttttgcta
     2521 caggagcggc agttgccgga gttgcttcag cagaggcagc tgctaccggg ccacgggcaa
     2581 cgactttctt catcgcatcg ccgatggatt ctgctttcgc gccaacaatc acctgaatag
     2641 tctgtttgtt cagttttact accccagaag cccccagacg tttacacatc gtatcgttaa
     2701 cgcgggcaga gtcagccacg gtaaggcgca gacgggtgat acaggcgtca attgctttca
     2761 ggttgtcagt gccgccaacc gcagcaatat agttggtcgc cagttgattg aggccttctt
     2821 cagtgttgct gttggcttct tcagtaacga tctcgtcttc tttatcttca cgacccggcg
     2881 ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac gaagtagata gcgaagaaga
     2941 caacacccat aaccagcagc atccagacgt tctggctggc ggccggcagg ttatacatca
     3001 acgcgtagtc gatagccccc gcagagaaag agaagccggc atggataccc agcagcgttg
     3061 ccacaaacag gctgataccg gtcagcagtg cgtgcaggag gtacagcagc ggagcaagga
     3121 acatgaacag gaattccagc ggctcagtca caccggtcag gaacgcagta acagcaacag
     3181 aaagcagcat cccgccaacc atcggacgac gctctttcgg tgctgcgaag tacatcgcca
     3241 gcgccgcacc cggcaggccg aacatcatga tcgggaagaa gccggacatg aacatccccg
     3301 cggtgccgtc accagcgtag aaacggttga tgtcgccgtg gaaaaccgta cccgccgcgt
     3361 tggtgaattc accaatctgg aaccaggcga tggtgttcag cacctgatgc agaccggttg
     3421 ggatcaacag acggttgatg aaaccaaaga taccggaacc cagcgcgccc gcagaaacga
     3481 tccactcgcc gcctgcatgg atagcgtgct gtaccggcgg ccagacgtaa ccaaaaatgg
     3541 ccgccagcac cagacagaag aagccggtgg cgatcggcac aaagcgtttg ccgccgaaga
     3601 agctcaggaa gtccggcagt ttaatatcgg accaacggtt ataggctgcg ccaccaacca
     3661 gaccggtaat gatacccgcc agtacaccca tgttaatttc tgggttgatg gtcaccatcg
     3721 ctttggttaa cacaaagtaa cctaccgccc ccgccagcgc cgccgcacct gcgctgtctt
     3781 tcgaccagct ggatgccaca ccgatggcga agattaatgc gaggttatca aaaatcgcac
     3841 cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg ctgaccgaat cgcagcaaca
     3901 gtgccgccac cggcagcacc gcgataggga gctgtaacgc cctaccgagt cgctggaaaa
     3961 aacctaaaat attcatctta ttccccctac gagaacccta tttggctcgt ttaaagccgt
     4021 atttttattg tgctgcaaat tgtactgccg atgttctgta atcagattgt tagatcatct
     4081 gccacagagt gtgtgaaaat ttaattcgta tcgcaaatta aacgagtgtc ttttgtgagt
     4141 tttgtcacca aatatcgtta tcatcactcc cttttactgg ctaaaccaga aaacttattt
     4201 tatcattcaa aaaatcaggt cggattgacg cctgtctgcg caaatccagg ttacgcttaa
     4261 agatgcctaa tccgccaacg gcttacattt tacttattga ggtgaataat gagactgatc
     4321 cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc gccatatcgt caatcgtatc
     4381 aatgcgttca aaccgactgc tgatcgtccg tttgttctgg gcctgccaac tggcggcacg
     4441 ccaatgacca cctataaagc gttagtcgaa atgcataaag caggccaggt cagctttaag
     4501 cacgttgtca ccttcaacat ggacgaatat gtcggtctgc cgaaagagca tccggaaagc
     4561 tactacagct ttatgcaccg taatttcttc gatcacgttg atattccagc agaaaacatc
     4621 aaccttctca acggcaacgc cccggatatc gacgccgagt gccgccagta tgaagaaaaa
     4681 atccgttctt acggaaaaat tcatctgttt atgggcggtg taggtaacga cggtcatatt
     4741 gcatttaacg aaccggcgtc ttctctggct tctcgtactc gtatcaaaac cctgactcat
     4801 gacactcgcg tcgcgaactc tcgtttcttt gataacgatg tcaatcaggt gccaaaatat
     4861 gccctgactg tcggtgttgg tacactgctg gatgccgaag aagtgatgat tctggtgctg
     4921 ggtagccaga aagcactggc gctgcaggcc gcagttgaag gttgcgtgaa ccatatgtgg
     4981 accatcagct gtctgcaact gcatccgaaa gccatcatgg tgtgcgatga gccttccacc
     5041 atggagctga aagttaagac tttaagatat ttcaatgaat tagaagcaga aaatatcaaa
     5101 ggtctgtaat tgttatccct gccctggctc cttgctcagg gcaatatttt ttaaaatcgg
     5161 gggtcagaat gtatgcatta acccagggcc ggatctttac cggccacgaa tttcttgatg
     5221 accacgcggt tgttatcgct gatggcctga ttaaaagcgt ctgtccggta gcggaactgc
     5281 cgccagagat cgaacaacgt tcactgaacg gggccattct ctcccccggt tttatcgatg
     5341 tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac cgctgaagcg gtcagcgtgg
     5401 aaacgctgga aatcatgcag aaagccaatg agaaatcagg ctgtactaac tatctgccga
     5461 cgcttatcac caccagcgat gagctgatga aacagggcgt gcgcgttatg cgcgagtacc
     5521 tggcaaaaca tccgaatcag gcgttaggtc tgcatctgga aggcccgtgg ctgaatctgg
     5581 tgaaaaaagg tacccataat ccgaattttg tgcgtaagcc tgatgccgcg ctggtcgatt
     5641 tcctgtgtga aaacgccgac gtcattacca aagtgaccct ggcaccggaa atggttcctg
     5701 cggaagtcat cagcaaactg gcaaatgccg ggattgtggt ttctgccggt cactccaacg
     5761 cgacgttgaa agaagcgaaa gccggtttcc gcgcggggat tacctttgcc acccatctgt
     5821 acaacgcgat gccgtatatt accggtcgtg aaccgggcct ggcgggcgcg atcctcgatg
     5881 aagctgacat ttattgcggt attatcgctg atggcctgca tgttgattac gccaacattc
     5941 gtaatgctaa acgccttaaa ggcgacaaac tgtgtctggt taccgacgcc accgcgccag
     6001 caggtgccaa cattgaacag ttcatttttg cgggtaaaac aatatactac cgtaacggac
     6061 tttgtgtgga tgagaacggt acgttaagcg gttcatcctt aaccatgatt gaaggcgtgc
     6121 gtaatctggt cgaacattgc ggtatcgcac tggatgaagt gctgcgtatg gcgacgctct
     6181 atccggcgcg tgcgattggc gttgagaaac gtctcggcac actcgccgca ggtaaagtag
     6241 ccaacctgac cgcattcaca cctgatttta aaatcaccaa gaccatcgtt aacggtaacg
     6301 aggtcgtaac tcaataagag aaagtatgac accaggcgga caagctcaga taggtaatgt
     6361 tgatctcgta aaacagctta acagcgcggc ggtttatcgc ctgattgacc agtacgggcc
     6421 aatctcgcgg attcagattg ccgagcaaag ccagcttgcc cccgccagcg taaccaaaat
     6481 tacgcgtcag cttatcgaac gcgggctgat caaagaagtt gatcagcagg cctccaccgg
     6541 gggccgccgc gctatctcta tcgtcaccga aacccgcaat ttccacgcaa tcggcgtacg
     6601 gcttggtcgt cacgacgcca ccatcaccct gtttgatctc agcagcaaag tgctggcaga
     6661 agaacattac ccgctgccgg aacgtaccca gcagacgctg gaacatgccc tgctgaatgc
     6721 cattgctcag tttattgata gctaccagcg caaactgcgc gagctgatcg ccatttccgt
     6781 tatcctgcca ggacttgttg acccggacag cggcaaaatt cattacatgc cgcatattca
     6841 ggtagaaaac tgggggctgg tagaagcact ggaagagcgt tttaaagtga cctgtttcgt
     6901 tggtcacgat atccgtagtc tggcgctggc ggagcactac ttcggtgcaa gtcaggattg
     6961 cgaagactcc attctggtgc gtgtccatcg cggaaccggg gccgggatta tctctaacgg
     7021 gcgcattttt attggccgca acggcaacgt cggtgaaatt ggccatattc aggtcgaacc
     7081 gctgggtgaa cgctgccact gcggcaactt tggctgcctg gaaactatcg ctgccaacgc
     7141 cgctattgaa caacgggtgt tgaatctgtt aaagcagggc taccagagcc gcgtgccgct
     7201 ggacgactgc accatcaaaa ctatctgcaa agccgcgaac aaaggcgata gcctggcctc
     7261 ggaagtgatt gagtatgtcg gtcgtcatct gggcaaaacc atcgccattg ctatcaactt
     7321 atttaatccg caaaaaattg ttattgccgg tgaaatcacc gaagccgata aagtgctgct
     7381 ccctgctatt gaaagctgca ttaatactca ggcgctgaag gcgtttcgta ctaatctgcc
     7441 ggtggtacgt tctgagctag accaccgctc ggcaatcggt gcttttgcgc tggtaaaacg
     7501 cgccatgctc aacggtattt tgctccagca tttgctggaa aattaatgtg cttttatagt
     7561 ggcgcttatt gttgtcaata ttctgggtag tccatgacca ttaaaaatgt aatttgcgat
     7621 atcgacggcg tgctgatgca cgataacgtc gccgtaccgg gtgcagcgga atttttgcac
     7681 gggattatgg ataaaggcct gccgctggtg ttgctgacca actatccttc gcagaccggg
     7741 caagatctgg cgaaccgctt tgccactgcc ggtgtcgatg tacctgacag cgtgttttat
     7801 acctctgcga tggcgaccgc cgatttcctg cgtcgccagg aaggcaagaa agcgtatgtt
     7861 gtgggcgaag gcgcactgat tcatgaactg tacaaagccg gtttcactat taccgatgtg
     7921 aaccctgatt ttgtgattgt tggcgaaacg cgttcctaca actgggacat gatgcataaa
     7981 gcggcctatt tcgtcgctaa cggtgcacgc tttatcgcca ccaacccgga cacccacggg
     8041 cgcggttttt atccggcctg tggcgcgttg tgtgcaggga ttgagaaaat ctccgggcgc
     8101 aaaccgttct atgttggtaa gccgagtccg tggattatcc gcgcggcatt aaacaaaatg
     8161 caggcacatt cggaagaaac ggtgattgtc ggcgataacc tgcgtaccga tattctggcc
     8221 ggcttccagg caggtctgga gacgattctg gtgctttctg gtgtttcgtc gctcgacgat
     8281 atcgacagta tgcctttccg ccccagctgg atttacccgt cagtcgctga aatcgacgtt
     8341 atctgaatag caaccacgtc tcaggacgtg gtttcttatt tttacccatc attaaaatca
     8401 tcccattcaa taaaatccgc caaaaattat cgatccatca ataaatacat caccgcaccc
     8461 tccccttttt cacaaatcag caatcaccat tacgtttttt attttttcac cgccaaatcg
     8521 cttgcgcaca gtgctgcttt gcggcatttt tttaaacaag caaacacaac aagcaacaaa
     8581 taccaggtta acggagaagg ttatgtgttc aatttttggc gtattcgata tcaaaacaga
     8641 cgcagttgag ctgcgtaaga aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc
     8701 ggactggtcc ggtatttatg ccagcgataa agccattctc gctcacgaac gtctgtcaat
     8761 tgttgacgtt aacgcggggg cgcaacctct ctacaaccaa caaaaaaccc acgtactggc
     8821 ggtaaacggt gaaatctaca atcaccaggc attgcgcgct gaatatggcg atcgttatca
     8881 gttccagaca gggtctgact gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga
     8941 atttctcgac gacttgcagg gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc
     9001 ctacctgatt ggtcgcgacc atctggggat catcccactg tatatgggct atgacgaaca
     9061 cggtcagctg tatgtggcct cagaaatgaa agccctggtg ccagtttgcc gcacgattaa
     9121 agagttcccg gcggggagct atttgtggag tcaggacggc gaaattcgtt cttattatca
     9181 tcgcgactgg ttcgactttg atgcagtgaa agataacgtg accgacaaaa acgagctgcg
     9241 tcaggctctg gaagattccg ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct
     9301 gctttctggt ggtctggatt cctcaattat ttccgctatc accaagaaat acgcagcccg
     9361 tcgcgtggaa gatcaggaac gctctgaagc ctggtggccg cagttacact cctttgctgt
     9421 aggtctgccg ggttcaccgg atctgaaagc agcccaggaa gtggcaaacc atctgggcac
     9481 tgtgcatcat gaaattcact tcactgtaca ggaaggtctg gacgccatcc gcgacgtgat
     9541 ttaccacatc gaaacctatg atgtgaccac tattcgcgct tcaacaccga tgtatttaat
     9601 gtcgcgtaag atcaaagcga tgggcattaa aatggtgctg tccggtgaag gttctgatga
     9661 agtgttcggc ggttatcttt acttccacaa agcaccgaat gccaaagaac tgcatgaaga
     9721 gacggtgcgt aaactgctgg ccctgcatat gtatgactgc gcgcgtgcca acaaagcgat
     9781 gtcagcctgg ggcgtggaag cacgcgttcc gttcctcgac aagaaattcc tcgacgtggc
     9841 gatgcgcatt aacccgcagg ataaaatgtg cggtaacggc aagatggaaa aacacatcct
     9901 gcgtgaatgt tttgagtcat acctgcccgc aagcgtggcc tggcggcaga aagagcagtt
     9961 ctccgatggc gtcggttaca gttggatcga caccctgaaa gaagtggcgg cgcagcaggt
    10021 ttctgatcaa cagctggaaa ctgcccgctt ccgcttcccg tacaacacac cgacctcaaa
    10081 agaagcgtat ctgtaccggg agatctttga agaactattc ccgcttccga gcgccgctga
    10141 gtgcgtgccg ggcggtcctt ccgtcgcgtg ttcttccgct aaagcgatcg aatgggatga
    10201 agcgttcaag aaaatggacg atccgtctgg tcgcgcggtt ggtgttcacc agtcggcgta
    10261 taagtaagaa atgcgaaaca gccccggtga aatccggggc tgtttcagtt attgaggtgc
    10321 ttgttgattc tgatatgcag taaggaaatg ttccgggttt gccacatcac tgtttgacaa
    10381 ctcatcactg taggccagta tttcatagat taatttccca ccactattgt taaattccga
    10441 atttcatcct gtatgacaaa agaatggctg tagggtggga aaaaacacaa agtaaacaat
    10501 aattgacgaa tatagcgcca cgctgttcgc aacctaacca aacagtcact ttcgagcaat
    10561 tttccttgaa aaagaggttg acgctgcaag gctctatacg cataatgcgc cccgcaacgc
    10621 cgataaggta tcgcgaaaaa aaagatggct acgtagctca gttggttaga gcacatcact
    10681 cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccatcttttt tttgcgggag
    10741 tggcgaaatt ggtagacgca ccagatttag gttctggcgc cgcaaggtgt gcgagttcaa
    10801 gtctcgcctc ccgcaccatt caccagaaag cgttgtacgg atggggtatc gccaagcggt
    10861 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatctt
    10921 ctttgagtaa gcggttcacc gcccggttat tggggtatcg ccaagcggta aggcaccggt
    10981 ttttgatacc ggcattccct ggttcgaatc caggtacccc agccatcgaa gaaacaatct
    11041 ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca caggttcgaa
    11101 tcccgtcgta gccaccaaat tctgaatgtg tcgaatacgt tcggcaaatt caaaaaccaa
    11161 tttgttgggg tatcgccaag cggtaaggca ccggattctg attccggcat tccgaggttc
    11221 gaatcctcgt accccagcca atttattcaa gacgcttacc ttgtaagtgc acccagttgg
    11281 ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc
    11341 gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc tgcgttcact
    11401 caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga cgctgccgcg
    11461 tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca accctaacgc
    11521 atatttcagc gcctgacgtt tcaacacgcc agcacgctcc gccgccatca aaccaagatt
    11581 acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca gatccatacc
    11641 gctctgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga ggacaggata
    11701 actggcccac gcttcgccgt agctgcgggc attcaccaga acatcaatca gggcatcgac
    11761 atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtgt gcgcggcatc
    11821 acccaccagc gcaagccccg gctgcacata ctgcaacgca tggcggcgtg tcagcggaaa
    11881 cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct tcgcgatttc
    11941 cacctgtaac tgcgccatat tcatattctg caactggcga atgcgcgccg gagagtcgta
    12001 ccacaccagc gatgcccagt tatcaaacag cggcagaaac gcgcgcggtc catccggggt
    12061 aaattgctgc caggtgctat cgccgggatc gttctcgcac tggacgctaa tcaacatgca
    12121 cgactgcgca tactgccatg catgaacgcc aattcccgcc atctgccgca cctgcgaatt
    12181 tgcgccgtcg gcaccaatca ccagcttcgc gcgaatcact tcaccgcctt tcagctccag
    12241 ctcctgaaga tcattatctc gatgtaatgc aatcagcgag gttggcacac gtaacgttac
    12301 tttcggatgc gcttccagcg cctgccacaa tgcctgttgc aggacagtgt tttctaccat
    12361 atagccaagc aacggcaact ttaattcggc tgcgtcaaat accacatgcg ccgtttccca
    12421 ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg catcccagac
    12481 ccctaaccct ttaagcaatg ataccgaagc cgcgctgatc gccgagatcc gcacgtccgg
    12541 ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta ccgtaaatcc
    12601 gtgctgtgcc agccccagcg ccagtgcgcc gccgaccatt cctccgccga caatggcaat
    12661 ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt aagtttacag
    12721 gatttttacc cccaagggct gataacgctc atactggtca caacggcagc aaagcattac
    12781 actatgcgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga ccaaaaaact
    12841 ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga tggccgatct
    12901 gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg atgtgctgct
    12961 gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc agttgggtcg
    13021 ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg gctgcgtggc
    13081 atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta tttttgggcc
    13141 gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc gcagcccggt
    13201 tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac cgcgcgccga
    13261 agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca cctactgcgt
    13321 ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc tgtttgagat
    13381 tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga acgtgaacgc
    13441 ctggcgtggt gaaaactacg acggcaccac cggatcgttt gccgatctgt tgcgtctggt
    13501 tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga tcgaattcac
    13561 cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc tgcatctgcc
    13621 ggtacagagc ggttccgatc gcattctgaa cctgatggga cgtacccata cggcgctgga
    13681 gtacaaagcg atcatccgta aactgcgtgc ggcgcgtcca gatattcaga tcagttctga
    13741 cttcatcgtc ggcttccctg gcgaaaccac cgaagacttc gagaaaacga tgaagctgat
    13801 tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc cgggtacacc
    13861 agccgccgat atggttgatg atgttccgga agaagagaag aagcagcgtc tgtatattct
    13921 gcaagaacgt atcaatcagc aagcgatggc gtggagccgc cggatgctcg gcaccaccca
    13981 gcgtattctg gtagaaggta catcgcgtaa gagcatcatg gagctttccg ggcgtacgga
    14041 aaataaccgc gtggtcaact tcgaaggcac gccggatatg atcggtaaat tcgtcgatgt
    14101 agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta ctgaagatga
    14161 aatgggtctg cgcgtggcag aaacaccaga atcggtgatt gcccgtactc gcaaagaaaa
    14221 cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc ggtgggcctt
    14281 gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc ctgtggcgtg
    14341 aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga taagaggaac
    14401 ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc gcgtctgttg
    14461 agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct cggcatcgag
    14521 atcaatcgcc gcgataacca ctttaaactg accggccgtc cgatttgcgt caccgctgcg
    14581 gcagacattc tgcgtagcct gtatgtcgat actgccccga tgcgcggtca gattcaggat
    14641 atcgaaccgg aacagatcca ccttgcgatt aaagaagcac gggtactgga gcaaagcgcg
    14701 gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg cgtaattaag
    14761 ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga cattaccttc
    14821 ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc agttgatgcc
    14881 ctggagcgtc aggaaattcg ccgtattctg ctgactcgtc cggcagtaga agccggtgag
    14941 aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct gcgcccgctg
    15001 tacgacgcgc tgtttgaaat gctgggtttt gagaaagtcg agaaactgat tgagcgcaac
    15061 gttattgaag tcgcaccgct ggcctatatg cgtggtcgta cgctgaacga cgcatttatc
    15121 attctcgatg agagccagaa cactaccatc gaacagatga agatgttcct gacccgtatc
    15181 ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct gccgcgtaat
    15241 actaaatcag gcttacgtca tgccatcgaa gtgctggccg atgtcgaaga gatcagcttt
    15301 aacttcttcc acagcgaaga cgtggttcgt cacccggtgg tggcgcgtat cgttaacgcc
    15361 tatgaagcct gggaagaagc cgaacaaaaa cgtaaagcgg cgctggcggc agaacgcaag
    15421 cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg gcatgtgaag
    15481 ataactccgg attgccggaa gagagccagt ttcaaacatg gctgaatgcg gtgatcccgc
    15541 agtttcagga agagtcggaa gtgacgattc gcgtggtcga taccgccgaa agccacagtc
    15601 tcaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc ccgtttgaag
    15661 tgccgcctgg catggagatg tcgctactgg gcgatctggt tatctgccgt cagatggttg
    15721 agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat atggtggtgc
    15781 acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca gaagaaatgg
    15841 aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac attgccgaga
    15901 aagaataatt tgccagccat ttgactggca gacgtccccg ccgttggtga ttaacgacgg
    15961 cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca attcacacag
    16021 tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc aacttttcca
    16081 cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg ggcagaacga
    16141 ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg cagaccaacg
    16201 cgtccgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca accagacgct
    16261 ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg tgattagcga
    16321 agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt ttatgcgcag
    16381 cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg ttcctgaaag
    16441 taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca tggcgatcgt
    16501 tattgacgaa ttcggtgggg tttccggtct ggtaaccatt gaagacatcc tggaactgat
    16561 tgttggtgag attgaagacg aatatgacga agaagatgat atcgacttcc gtcagctgag
    16621 tcgtcatacc tggaccgtgc gcgcactggc ttccattgaa gacttcaacg aagcgttcgg
    16681 cacccacttt agcgatgaag aagtcgacac tatcggtggt ctggtgatgc aggcatttgg
    16741 gcatcttccg gcacgtggcg aaaccatcga catcgacggt taccagttca aagtggcgat
    16801 ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact caccccagcc
    16861 gaagctggat gaataaaacc gaaactggat agataactac atggcttttg cctcattaat
    16921 tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg gaacgctggc
    16981 cttctctcct tacgacgtct ggcctgcggc gattatttcg ctgatggggc ttcaggcgtt
    17041 gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg gatttggcct
    17101 ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg gaatgcctgg
    17161 cccggttaac atcttcctgg tggtactgct ggcggcgtat ttgtcgctgt ataccggact
    17221 gtttgctggc gtgctgtcgc gcctgtggcc gaaaaccacc tggctgcgcg tggcgattgc
    17281 cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga caggcttccc
    17341 gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg caccgctaat
    17401 gggcgtggaa gccattaact tcctgcttat gatggttagc ggcctgctgg cactggcgtt
    17461 agtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc ttcccttccc
    17521 gctgcgttac atccggtggt ttaccccaca accggagaaa accattcagg tttcgatggt
    17581 tcagggcgat attccgcaat cgctgaaatg ggacgaaggc cagcttctta atacgctgaa
    17641 gatttactac aacgcaacgg caccgctgat gggcaaatca tcgttgatta tctggccgga
    17701 gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac tggacggtga
    17761 gttgcgtgat aaaggtagct cgctggtgac cgggattgtt gacgcgcgtc ttaataagca
    17821 gaaccgttac gatacctaca acaccatcat cacactgggt aaaggtgcac cgtacagcta
    17881 cgaatcagcc gatcgctata acaaaaacca tctggtgccg tttggcgagt ttgtcccgct
    17941 ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt cgttcagccg
    18001 tgggccatat atccagccgc cgctgtcggc aaatggtatt gagcttactg cggctatttg
    18061 ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata ccgactatct
    18121 gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc agcacttcca
    18181 gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca ccaacaacgg
    18241 cattacggcg gtgattggcc cgcagggtga gattcaggcg atgatcccgc agttcacccg
    18301 cgaggtgtta accactaacg tgacgccgac caccggactc acaccatacg cacgtaccgg
    18361 caactggccg ctgtgggtgc tgacggcact gtttggtttt gctgctgtgt tgatgagtct
    18421 gcgtcagcga cgtaaataat cccttcttat cgtgccggaa tacatcccat atttcggcac
    18481 gctcaccgga tgcggcatca gtgcattatc cggataacaa tattctccta tccaactttg
    18541 gcacatctat tgctttgtta tacaaggcaa agctttaacc agcatcagta caacccggtt
    18601 gcaccacagc aatccaccgg tagcaccaaa agagtgcatt ttaagaattt tgcttcggaa
    18661 tggtgcggcg cacctcccaa atataaacaa taaaacagca atatctttac atttagccaa
    18721 actaaatgtt acctaacagg cacaacacta cacaataaag ttgcagatga taacaacaca
    18781 aacactcaca acgggtatcc atgcgttctt aacgcagaag ataaaggagt tggatatgca
    18841 attacgtaaa cctgccacag caatcctggc cctggcgctt tccgcaggac tggcacaggc
    18901 agatgacgcc gccccggcag cgggcagtac gctggacaaa atcgccaaaa acggcgtgat
    18961 tgtcgtcggt caccgtgaat cttcagtacc tttctcttat tacgacaatc agcaaaaagt
    19021 ggtgggttat tcgcaggatt actccaacgc cattgttgaa gcagtgaaaa agaaactcaa
    19081 caaaccggac ttgcaggtga aactgattcc gattacctca caaaaccgta ttccgctgct
    19141 gcaaaacggc acttttgatt ttgaatgtgg ttctaccacc aacaacgtcg aacgccaaaa
    19201 acaggcggct ttctctgaca ctattttcgt ggtcggtacg cgcctgttga ccaaaaaggg
    19261 cggcgatatc aaagattttg ccgacctgaa aggcaaagcc gtagtcgtca cttccggcac
    19321 tacctctgaa gttttgctca acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat
    19381 cagcgccaaa gatcacggtg actctttccg taccctggaa agcggtcgtg ccgttgcctt
    19441 tatgatggat gatgctctgc tggccggtga acgtgcgaaa gcgaagaaac cagacaactg
    19501 ggatatcgtc ggcaagccgc agtctcagga agcctatggt tgcatgttgc gtaaagacga
    19561 tccgcagttc aaaaagctga tggatgacac tatcgctcag gtgcagacct ccggtgaagc
    19621 ggaaaaatgg tttgataaat ggttcaaaaa tcccattccg ccgaaaaacc tgaacatgaa
    19681 tttcgaactg tcagacgaaa tgaaagcact gttcaaagaa ccgaatgaca aagcactgaa
    19741 ctaattacaa gaaccagggg cggaaattct agccctctcg attgttacgt agcacggaca
    19801 gactatacgc ctgatggtcg ttccccatcg ggcctgaaaa ccgcaatacg ctgggtaaca
    19861 atcttcgagg gtagcagtta acgctgctac cctctttttt ctggagtaga tttatgtcta
    19921 tagactggaa ctggggtatt tttttacaac aagccccgtt cggcaacacc acctatcttg
    19981 gttggatctg gagtggtttt caggtgacga tcgctttgtc gatctgcgcc tggattatcg
    20041 ctttcctcgt cggttcattt ttcggtattt tacgtaccgt tccaaaccgt tttctctccg
    20101 gtctgggtac gttgtatgtc gaactgttcc gcaacgtgcc gttgattgtg caattcttta
    20161 cctggtatct ggtgatcccg gagctgctgc cggagaaaat cggcatgtgg tttaaggccg
    20221 agctggatcc caatattcag tttttccttt catccatgct ctgcctgggg ctgtttaccg
    20281 ccgcccgtgt ttgcgaacag gtacgcgccg cgattcagtc gctgccgcgt gggcagaaga
    20341 atgccgcgct ggctatgggt ctgacgctgc cgcaggctta ccgctacgta ctgctgccta
    20401 atgcttatcg cgttatcgtc ccgccgatga cctcagagat gatgaacctg gtgaaaaact
    20461 ccgccatcgc ctcgaccatc ggtctggtag atatggcagc gcaggcgagt aaattgctcg
    20521 attactcggc tcacgcctgg gagtcgttta ccgccattac gctagcgtat gtgtttatta
    20581 acgcttttat catgctggtg atgacgctgg ttgaacgtaa agttcgcctg cctggcaaca
    20641 tggggggcaa ataatgtacg agtttgactg gagttccatt gtcccttccc tgccatatct
    20701 gctcgacggg ctggtgatca cgctgaaaat caccgtcacg gcagtcgtga ttggtatttt
    20761 gtggggcacc atgctggcgg tgatgcgctt gtccagcttt gcgcccgtag cgtggtttgc
    20821 caaagcctac gttaacgtat tccgctcgat tcctttagtt atggttttgc tgtggtttta
    20881 cctgatcgtg ccgggttttc tgcaaaacgt gctgggatta tcaccaaaaa atgatatccg
    20941 tttaatttcg gcgatggtgg cgttttcgat gtttgaagcg gcctactatt cagagattat
    21001 ccgtgccggt attcaaagta tttctcgtgg tcagtcgagc gccgcgctgg cgttgggaat
    21061 gactcactgg cagtcgatga aactgattat tctgccgcag gcgttccgcg cgatggtgcc
    21121 gctgctgctc actcagggca tcgtactgtt ccaggatacc tcgctggtgt atgtgttatc
    21181 tctggccgat ttcttccgta ccgcctcaac cattggtgag cgtgatggca cgcaggttga
    21241 gatgatcctg tttgccggat ttgtttattt cgttattagc cttagcgcgt cgttgttggt
    21301 cagctacttg aaaagaagga cagcataatg attaccctga aaaatgtttc aaaatggtat
    21361 ggtcactttc aggtgctgac cgactgctca accgaagtga aaaaaggcga agtggtggtg
    21421 gtttgcggcc cgtctggttc cggcaaatca acgctgatta aaaccgtcaa cggcctcgaa
    21481 ccagtgcagc aaggtgaaat caccgtcgat ggtatcgtgg ttaacgacaa gaaaaccgat
    21541 ctggcaaagc tgcgttctcg cgtcgggatg gtattccaac atttcgagct gttccctcat
    21601 ctgtcgatta tcgaaaacct gaccctggcg caggtgaaag tgcttaaacg cgataaagca
    21661 ccggctcgtg aaaaagcatt gaaattgctg gagcgcgtgg gactttccgc acatgccaat
    21721 aaatttccgg cgcagctttc cggcggccag cagcagcgtg tggcaattgc tcgcgcattg
    21781 tgtatggatc ctattgcgat gctgttcgac gaacccacat cggcactaga cccggagatg
    21841 atcaacgaag tactggacgt gatggtggaa ctggcgaacg aggggatgac catgatggtg
    21901 gtgacccacg aaatgggctt tgcccgtaaa gtggcgaatc gggtgatctt tatggatgag
    21961 ggtaaaattg ttgaagactc gccgaaagat gcgttcttcg atgatccgaa atcggaccgc
    22021 gcaaaagact tcctcgcgaa aatcctgcat taatcactct ggcgcgcata cccaagtgcg
    22081 cgccatctca cgctttatcg tctcgcccct cgtatctgtt acatcctggc gttacccttg
    22141 tcgcaaagaa gcacacaaca aggagcaaca atggcactgc caattctgtt agattgcgac
    22201 ccaggtcatg atgacgctat cgcaatagtt ctcgccctcg cctcaccaga gcttgatgtc
    22261 aaagcaatta cgtcttccgc cggaaaccag acaccagaaa aaaccttacg caatgttctg
    22321 cgtatgctga ccttgcttaa tcgcaccgat attccggtgg caggcggcgc ggtaaaaccg
    22381 ttaatgcgtg agttgattat cgcggacaat gtgcacggcg aaagcggtct cgacggcccg
    22441 gcattaccgg aaccgacgtt cgcaccgcaa aactgtacgg cggtagagct gatggcgaaa
    22501 acgctgcgtg aaagtgcgga acctgtcacg attgtgtcta ccggaccgca aactaacgtt
    22561 gccttgctgc tcaatagcca tccggaactg catagcaaaa ttgcccgtat cgtgattatg
    22621 ggtggcgcta tgggactggg taactggacg cctgcggctg aatttaatat ttacgttgac
    22681 ccggaagcgg cagaaattgt cttccagtca gggatcccgg tggtgatggc cggtctggat
    22741 gttactcaca aagcacaaat ccacgttgaa gacactgagc gtttccgcgc gattggcaac
    22801 cctgtttcaa ccattgttgc cgaactgctg gatttcttcc tcgaatatca taaagacgaa
    22861 aaatggggct ttgtcggcgc accgctacat gacccatgca ccatcgcctg gctgttaaaa
    22921 ccggagttgt ttaccactgt tgagcgctgg gttggcgtgg aaacacaggg gaaatatacc
    22981 cagggtatga cggttgttga ttattattat ctgactggca ataaaccgaa tgccactgta
    23041 atggtcgatg ttgatcgtca gggctttgtt gatttactgg ctgatcgtct gaaattttac
    23101 gcttaacaca cgtcattgat taagatcacc acgcgttacc tgggtggtct taatctgttt
    23161 atctcttttt ctttcaagat ataaatgcaa tggataatgc agaactcgcc attggtatcg
    23221 atctcggtac taccaatagt ttaattgccg tctggaaaga cggtgccgcg caattaattc
    23281 caaataagtt cggtgaatat ttaacaccat ccataattag catggatgaa aataatcata
    23341 ttttagtcgg aaaaccggct gtatcacggc gtacttcgca tccggataaa acggcagcgt
    23401 tatttaaacg tgcaatgggc agtaatacca actggcggtt aggcagcgac acatttaacg
    23461 cgccagaact gtcctctttg gtattacgct cattaaaaga agatgccgaa gaatttctgc
    23521 aacgtccgat taaagatgtg gtgatctctg ttccggctta tttcagcgat gaacaacgca
    23581 agcatacccg tttagcagcg gagttagccg gtttaaatgc ggtacgctta attaatgaac
    23641 ccacagcagc tgcgatggcg tatggcctgc atacccaaca aaatacccgt tcgctggtgt
    23701 ttgatctcgg tggcggcacg tttgacgtca cggtgcttga gtacgccacg ccggtgattg
    23761 aagttcacgc ctccgctggc gacaacttcc tcggtggcga agattttacc catatgctgg
    23821 tcgatgaggt tttaaaacgc gcggatgtcg ccaggaccac gcttaacgag agtgaactgg
    23881 cagccttgta tgcctgtgtg gaagcggcaa aatgtagcaa tcaatcgcca atacacattc
    23941 gctggcagta ccaggatgaa acgcgggaat gcgaatttta cgagaacgaa ctggaagatt
    24001 tgtggttgcc gctgctcaat cgcttgcgag tgccgattga acaggcgttg cgcgatgcgc
    24061 gtctgaagcc gagtcaaatc gacagtctgg tgctggttgg cggcgcgtca caaatgccgc
    24121 tggtgcagcg aatcgccgtg cgtctgtttg gcaaattacc gtatcaaagt tacgatccga
    24181 gcaccattgt cgcgctgggc gcagcaatcc aggcagcctg ccgcttacgc agtgaagata
    24241 ttgaagaagt gatcctcact gatatttgcc cttactcgtt gggcgttgag gttaaccgcc
    24301 agggcgtttc cggcattttc tcgccgatta ttgaacgaaa caccactgtg cccgtgtcgc
    24361 gtgtagaaac ttattcaacc atgcacccgg aacaggattc tattacggtt aacgtctatc
    24421 agggagaaaa ccacaaagtt aaaaacaaca ttctggtgga atccttcgat gtgccgttga
    24481 agagaaccgg ggcttatcag tcgattgata ttcgctttag ttatgatatc aacggtttgc
    24541 ttgaagttga cgtgcttctg gaagacggca gcgttaagtc cagagtgatt aaccacagcc
    24601 cggtaacatt gagcgcgcag cagattgaag agagtcggac gcggttatcc gcattgaaaa
    24661 tttatccgcg cgatatgctc atcaaccgca cctttaaggc caaactggaa gagttgtggg
    24721 cgcgggcgct gggtgacgag cgagaagaga ttggccgggt gatcaccgat tttgatgcgg
    24781 cgctgcagtc aaacgatatg gcccgcgtcg atgaggtcag gcggcaggcg agcgtttatt
    24841 tagccattga gatcccataa tcacggctgc cggatgcggc gtaaatgctg catccggcat
    24901 cggcgcaaaa aacacatcgt caactaaaca acatcgcgat tttgccgaga gcataaccta
    24961 tcaccagcac cgttaagctg attttgataa tgctggtaat gctctgtggt ttttcatcct
    25021 catcattttt ttcttcatgg ttttcaggtg ttggctcatc gacatcttca tcctccggct
    25081 cgagttttac tttcgtcaac agcatccggg tgataatggc ctctaatgga tgagcatctc
    25141 cgccttccag ccttgtatta agacgggcca gcatctcttg tttcggaaac gcttcaagta
    25201 ctacctgttc cgctacccga tacaaatcca gtaacggtga atcttcttct gtctcgctac
    25261 cgactctcga tttccaccac gcccatgctt tgcgtttgaa gaatagcgac tcaataaacg
    25321 attgacctaa cggcgatagt tttccgctat gtaaaatttc cgcccataac agacgcgtcc
    25381 gggcgctctc accaggaaca tatttcaacg gtttaccttg cgcatctacc atgctccgcg
    25441 cttcgataac catcactaat aacgcgaaat aatccgggca atgttgtgcg ctccactgca
    25501 acaacaaatt atcggcttgt gtagatgggt aacttcccca gtacgcgcag agatcggtaa
    25561 gcaggctttc cccctcgccg cagtacaaac gttgtgcaca ctggtaatac cgggcatctt
    25621 cgctttctgg ttcttccatt tgccactgtt cggctacagg aatgagttcc gccatacctg
    25681 tttgcaccga gctgtaccag cgcaacagtt tgcgatgtaa ctttgcatca tcaggaatga
    25741 accacggacc ttccatcgcc aaaaacgcag tgacatagtc aggatggtaa cggcagcaac
    25801 gttccagcgc gaagtaatac tcaacagtct ggttctgcaa cgcaacggga tataccagaa
    25861 ggccaaggaa atcgaaacaa tcaccagcct gaatggcctc aagaaaagcc tccctttctt
    25921 actcatctat tccttcgctg tcattaaacg aatgccagtt caaacgctct gccagcaaag
    25981 aagcacaatt taatgaaaga tatcgcgctt ctatagcgat tgcacacagc ggccagcgta
    26041 attgatcgac atcctccatg ttccaggtat ttaattgctg aataaatttc tgccaggcgg
    26101 agggctgaaa acgatcactt tctgaatcca gtaatgccct gaatacacgt agtatttcat
    26161 gctcagtggc atcatctttt tcttcatcat ctgcttcctc gacaggattt accgccagtc
    26221 gcagggcctc ttcataggcc tggcgtaatg ctttaaaccc ttgcggatcg gtttcgggat
    26281 gacataacgg taagcgagca agataagcct ggcggataat gtctatttgc gtcgtgcttt
    26341 caatttcgag gatttgccaa catgttttca taggtcgctc acatccttaa gcgttaccgg
    26401 gcattcaggc tggacagtta atggctgcca gggaagcgtg gcatagaaac gatcgcttcc
    26461 cgcagccagc tcttcgaaca tccgactccc ggcgctatcg tatttgtaca gggttagcgc
    26521 ctcgcgtagc gaagcgttgt cttcgcattg agaagattgc cagtagagac gccctagtga
    26581 ataggcggaa aaatagtgca tccagctgtg atagtaatgg tgagctcgtt catagacgcg
    26641 cgattgcagc cataaactct cgtcttccgt cagccacttg ttgagcacac cgacccgtga
    26701 cagaaacccc attcgaacat aatcccaagc tttgattccc ccttccccac aacacatagc
    26761 ggtttgggca acgaattggg tataaatcaa ccctctctcc gagccaccag caattaatgc
    26821 tttccactct tcaggggaag agcgatgcca tgtcagatat aagccagcta aatcattggc
    26881 atgaccatca tcagtcatct gataaatagt ctgaattaaa tcatcacgat tatttattcc
    26941 ccaggatgat gagagatcga attcttttat cccggtatac atttttggcg agtcatgcgc
    27001 gggtttgcaa ccctgattaa gtatttccat gggcgcagaa agcgcaaaaa gaagatattg
    27061 ttcttcctta ttcatttgtt aa
//
LOCUS       DAZKIF010000060        26971 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZKIF010000060 DAZKIF010000000
VERSION     DAZKIF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.060"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1306
                     /locus_tag="ACHOTW_002487"
     CDS             <1..1306
                     /locus_tag="ACHOTW_002487"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9844576.1"
                     /translation="PLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDENVHKLM
                     DLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGG
                     MAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPK
                     RMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFL
                     APFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGG
                     QIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFI
                     CTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIM
                     CRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1462..2997
                     /gene="gadC"
                     /locus_tag="ACHOTW_002488"
     CDS             1462..2997
                     /gene="gadC"
                     /locus_tag="ACHOTW_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP9844577.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3128..4447
                     /locus_tag="ACHOTW_002489"
     CDS             3128..4447
                     /locus_tag="ACHOTW_002489"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP9844578.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4824..6206
                     /gene="dosC"
                     /locus_tag="ACHOTW_002490"
     CDS             4824..6206
                     /gene="dosC"
                     /locus_tag="ACHOTW_002490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFP9844579.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            6231..8620
                     /gene="dosP"
                     /locus_tag="ACHOTW_002491"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             6231..8620
                     /gene="dosP"
                     /locus_tag="ACHOTW_002491"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            8878..9459
                     /gene="ddpX"
                     /locus_tag="ACHOTW_002492"
     CDS             8878..9459
                     /gene="ddpX"
                     /locus_tag="ACHOTW_002492"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP9844580.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            9473..11023
                     /locus_tag="ACHOTW_002493"
     CDS             9473..11023
                     /locus_tag="ACHOTW_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9844581.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            11025..12046
                     /locus_tag="ACHOTW_002494"
                     /pseudo
     CDS             11025..12046
                     /locus_tag="ACHOTW_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            12043..12939
                     /gene="ddpC"
                     /locus_tag="ACHOTW_002495"
     CDS             12043..12939
                     /gene="ddpC"
                     /locus_tag="ACHOTW_002495"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP9844582.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12936..13922
                     /locus_tag="ACHOTW_002496"
     CDS             12936..13922
                     /locus_tag="ACHOTW_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9844583.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13915..14841
                     /locus_tag="ACHOTW_002497"
     CDS             13915..14841
                     /locus_tag="ACHOTW_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9844584.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(14897..15328)
                     /gene="osmC"
                     /locus_tag="ACHOTW_002498"
     CDS             complement(14897..15328)
                     /gene="osmC"
                     /locus_tag="ACHOTW_002498"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP9844585.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15673..15888
                     /gene="bdm"
                     /locus_tag="ACHOTW_002499"
     CDS             15673..15888
                     /gene="bdm"
                     /locus_tag="ACHOTW_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP9844586.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15990..16127
                     /gene="sra"
                     /locus_tag="ACHOTW_002500"
     CDS             15990..16127
                     /gene="sra"
                     /locus_tag="ACHOTW_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP9844587.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            16284..17981
                     /gene="maeA"
                     /locus_tag="ACHOTW_002501"
     CDS             16284..17981
                     /gene="maeA"
                     /locus_tag="ACHOTW_002501"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9844588.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGISGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            18115..19125
                     /gene="adhP"
                     /locus_tag="ACHOTW_002502"
     CDS             18115..19125
                     /gene="adhP"
                     /locus_tag="ACHOTW_002502"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP9844589.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            19271..19555
                     /locus_tag="ACHOTW_002503"
     CDS             19271..19555
                     /locus_tag="ACHOTW_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9844590.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(19783..20436)
                     /gene="fdnI"
                     /locus_tag="ACHOTW_002504"
     CDS             complement(19783..20436)
                     /gene="fdnI"
                     /locus_tag="ACHOTW_002504"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP9844591.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(20429..21313)
                     /gene="fdnH"
                     /locus_tag="ACHOTW_002505"
     CDS             complement(20429..21313)
                     /gene="fdnH"
                     /locus_tag="ACHOTW_002505"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP9844592.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(21326..24373)
                     /gene="fdnG"
                     /locus_tag="ACHOTW_002506"
     CDS             complement(21326..24373)
                     /gene="fdnG"
                     /locus_tag="ACHOTW_002506"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23786..23788),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9844593.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24605..25486
                     /gene="yddG"
                     /locus_tag="ACHOTW_002507"
     CDS             24605..25486
                     /gene="yddG"
                     /locus_tag="ACHOTW_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP9844594.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            25745..26845
                     /gene="ompC"
                     /locus_tag="ACHOTW_002508"
     CDS             25745..26845
                     /gene="ompC"
                     /locus_tag="ACHOTW_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9844595.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRDNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         6637 a         6820 c         6791 g         6723 t
ORIGIN      
        1 tccgctgcac gaaatgcgcg acgatgtcgc atttcagatt atcaatgacg aattatatct
       61 tgatggcaac gctcgtcaga acctggccac tttctgccag acctgggacg acgaaaacgt
      121 ccacaagttg atggatttat ccattaacaa aaactggatc gacaaagaag aatatccgca
      181 atctgcagcc atcgacctgc gttgcgtaaa tatggttgcc gatctgtggc atgcgcctgc
      241 gccgaaaaat ggtcaggccg ttggcaccaa caccattggt tcttccgagg cctgtatgct
      301 cggcgggatg gcgatgaaat ggcgttggcg caagcgtatg gaagctgcag gcaaaccaac
      361 ggataaacca aacctggtgt gcggcccggt gcaaatctgc tggcataaat tcgcccgcta
      421 ctgggatgtg gagctgcgtg agatccctat gcgccccggt cagttgttta tggatccgaa
      481 acgcatgatt gaagcctgcg acgaaaatac catcggcgtg gtgccgactt tcggcgtgac
      541 ctacaccggt aactatgagt tcccgcaacc gctgcacgat gcgctggata aattccaggc
      601 cgacaccggt atcgacatcg acatgcacat cgacgccgcc agtggtggct tcctggcacc
      661 gttcgtcgcc ccggatatcg tctgggactt ccgcctgccg cgtgtgaaat cgatcagtgc
      721 ttcaggccac aaattcggtc tggctccgct gggctgcggc tgggttatct ggcgtgatga
      781 agaagcgctg ccgcaggaac tggtgttcaa cgttgactac ctcggtggtc agattggtac
      841 tttcgccatc aacttctccc gcccggcggg tcaggtaatt gcacagtact atgaattcct
      901 gcgcctcggt cgtgaaggct ataccaaagt acagaacgcc tcttaccagg ttgccgctta
      961 tctggcggat gaaatcgcca aactggggcc gtatgagttc atctgtaccg gtcgcccgga
     1021 cgaaggcatt ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct
     1081 gtatgacctc tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg
     1141 cggtgaagcc accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga
     1201 ctttgctgaa ctgttgctgg aagactacaa agcctccctg aaatatctca gtgatcaccc
     1261 gaaactgcag ggtattgccc aacagaacag ctttaaacat acctgataac gtttaacggt
     1321 aacggtgtcc cgaaacgaac ccgtttcggg acaatttcca aagtctgttc actggcatta
     1381 gcaacggaaa atattgttct gaatacgctt cagaacaaaa caggtgcggt tccgacagga
     1441 ataccgtttt agggggataa tatggctaca ttagtacaga caggtaaagc taagcagctc
     1501 acattactcg gcttttttgc cataacggca tcgatggtaa tggctgttta tgaataccct
     1561 accttcgcaa catcgggctt ttcattagtc ttcttcctgc tattaggcgg gattttatgg
     1621 tttattcccg tgggactttg tgctgcggaa atggccaccg tcgacggctg ggaagaaggt
     1681 ggtgtcttcg cctgggtatc aaatactctg gggccgagat ggggatttgc agcgatctca
     1741 tttggctatc tgcaaatcgc cattggtttt attccgatgc tctatttcgt gttaggggca
     1801 ctctcctaca tcctgaaatg gccagcgctg aatgaagacc ccattaccaa aactattgca
     1861 gcactcatca ttctttgggc gctggcatta acgcagtttg gtggcacgaa atacacggcg
     1921 cgaattgcta aagttggctt cttcgccggt atcctgttac ctgcatttat tttgattgca
     1981 ttagcggcta tttatctgca ctccggtgcc cccgttgcta tcgaaatgga ttcgaagacc
     2041 ttcttccctg acttctctaa agtgggcacc ctggttgtat ttgttgcctt cattttgagt
     2101 tatatgggcg tagaagcttc cgcaacccac gtcaatgaaa tgagtaaccc agggcgcgac
     2161 tatccactgg ctatgttact gctgatggtg gcggcaatct gcttaagctc tgttggcggt
     2221 ttgtctattg cgatggtcat tccgggtaat gaaatcaacc tctccgcagg ggtaatgcaa
     2281 acctttaccg ttctgatgtc ccatgtggca ccggaaattg agtggacggt tcgcgtgatc
     2341 tccgcactgc tgttgctggg tgttctggcg gaaatcgcct cctggattgt tggtccttct
     2401 cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag cggcattcgc taaaatgaac
     2461 aaaaatggcg taccggtaac gctggtcatt tcgcagctgg tgattacttc tatcgcgttg
     2521 atcatcctca ccaataccgg tggcggtaac aacatgtcct tcctgatcgc actggcgctg
     2581 acggtggtga tttatctgtg tgcttatttc atgctgttta ttggctacat tgtgttggtt
     2641 cttaaacatc ctgacttaaa acgcacattt aatatccctg gtggtaaagg ggtgaaactg
     2701 gtcgtggcaa ttgtcggtct gctgacttca attatggcgt ttattgtttc cttcctgccg
     2761 ccggataaca tccagggtga ctctaccgat atgtatgttg aattactggt cgttagtttc
     2821 ctggtggtac ttgccctgcc ctttattctc tatgctgttc atgatcgtaa aggcaaagcg
     2881 aataccggcg tcactctgga gccaatcaac agtcagaacg caccaaaagg tcacttcttc
     2941 ctgcacccgc gtgcacgttc accacactat attgtgatga atgacaagaa acactaacta
     3001 tgtctgaatg gttataagat aagggagcga taattcatcg ctcccttttt cgtgcttgct
     3061 gtcgtattga cttaaccggg ataagtacga gaatgagcgc acatctgttt accggaaact
     3121 agcacatatg gatatctgct cccgaaacaa gaaattaacg attagaagac cagcgatact
     3181 ggttgcactg gcacttttac tgtgtagttg taaaagcacg cctccagagt ccatggtgac
     3241 accaccagcg ggttcaaagc caccagccac gacgcaacaa tcgtcacaac cgatgcgtgg
     3301 catctggctg gccacggtgt ctcgcctcga ctggccaccg gtttcctcgg ttaacattag
     3361 taaccccacc agccgggccc gtgtacaaca acaggcgatg atcgacaaac tggatcattt
     3421 gcaacgtctc ggtataaaca cggtcttttt ccaggtcaag ccggacggta ccgccctgtg
     3481 gccatcgaaa attttgccgt ggtccgatct tatgaccggt aagattggtg aaaatccggg
     3541 ttacgatccg ctgcaattta tgctcgacga agcccacaag cgtgggatga aagtacacgc
     3601 ctggtttaac ccctatcgcg tatcggttaa tacgaagccc ggtactatca gggaactgaa
     3661 tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa caccgtgact ggatcagaac
     3721 gtctggcgat cgctttgtcc tcgacccggg catccctgag gttcaggact ggatcacatc
     3781 aatagtcgca gaagtggttt cccgctatcc ggtagatggc gtgcagtttg acgactattt
     3841 ctatactgag tcaccgggtt cacggctaaa tgataacgaa acgtaccgta aatacggagg
     3901 cgcatttgcg tcaaaagcag actggcggcg caacaatact cagcagttaa ttgcaaaggt
     3961 atcgcacacc attaaaagca ttaagccggg agtcgaattt ggtgttagcc cggcaggcgt
     4021 gtggcgtaac cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg cagcctatga
     4081 cgaatcctac gctgacactc gtcgatgggt ggaacaagga ttgctggatt acattgctcc
     4141 ccaaatttac tggccgttct cacggagtgc cgcgcgttat gacgtgttgg caaaatggtg
     4201 ggcggatgtc gttaaaccga ccaggacccg cctgtatatc ggtatcgcct tctataaagt
     4261 gggtgaacct tcaaagatag agccagactg gatgattaac ggcggcgtac cggagctgaa
     4321 aaagcagctc gatcttaacg atgcggtacc agaaattagc ggcaccatct tgttccgtga
     4381 ggactatctg aataaaccgc agactcaaca agcggtcagc tatctgcaaa gtcgttgggg
     4441 cagttaaact cccattctta ctttgtaaca agccggtgct ttacccactg gcttgttacc
     4501 ctcttgaact atcccaccag caatcatgct tactaaagtc aaattaacca cgcaattaac
     4561 cacgcttagt taaacagcca taaatcccct aataaaaatg tatgaatatg aaaaaccata
     4621 cgatattcgc agtcaccgaa aaataattac gatgggctat actcttctga agaacagtag
     4681 cgtagattga aactgtccta agtcgcttat ttcttttcag tatatcttca tatttcagga
     4741 gaatatggtg cagttagtcg atctcgcacg tcgcgtttca ttttgattcc gtctgatttc
     4801 ccagccttat aagggtgtga attatggaga tgtattttaa aagaatgaaa gatgagtgga
     4861 ccggacttgt cgagcaggca gatccgccca ttcgtgctaa agccgcggaa attgccgttg
     4921 cgcatgctca ttatctgagt atcgagtttt atcgaattgt ccgcatcgac ccgcatgccg
     4981 aagaattctt gagtaatgaa caagttgagc ggcagttgaa aagtgcgatg gaacgctgga
     5041 ttattaacgt gctttctgcc caggttgacg atgtcgaaag gctaatacaa atccagcata
     5101 ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga aattgtcgag atggggtttc
     5161 gggtgctgaa aaagatcctc tatccggtaa tcttctcttc ggattattct gccgcagaaa
     5221 aacttcaggt ctaccatttc tcgattaaca gtattgatat cgctatggaa gtgatgaccc
     5281 gcgcgtttac ctttagtgac agtagtgcct caaaagaaga tgaaaactat cgtatcttct
     5341 cgttactgga aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg
     5401 aaatagatat tatctataaa atcctgctgg attctgattt aggcagtagt ttgcctttaa
     5461 gccaggctga ttttggcctg tggtttaacc ataaaggtcg acattatttc agcggcattg
     5521 ctgaagtggg ccatatctct cgtctgattc aggatttcga cggtattttc aatcaaacca
     5581 tgcgtaacac caggaatttg aataacagaa gtctgcgggt gaaattttta ttacagataa
     5641 gaaataccgt atcgcaaatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc
     5701 acgaagtcgg tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct
     5761 tcaaacgcga aattgcccat gccaaccgga ccggtacacc gctgtcagtg ctgattattg
     5821 acgttgataa attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc
     5881 tgcgtaaagt ctctcaggcc ttttatgaca acgtccgcag tagtgattat gttttccgct
     5941 acgggggcga tgaatttatc attgttttga ctgaagcttc tgaaaacgaa acgttacgta
     6001 ccgcagaacg tattcgcagt cgggtggaga aaaccaaact gaaagccgca aacggcgaag
     6061 atattgccct ctcactttcc atcggtgccg ccatgtttaa tggtcatcct gactatgagc
     6121 gcctcattca aatagccgat gaagctctgt atatcgccaa aagacgaggt agaaaccgtg
     6181 ttgaactctg gaaagccagt ctttagatgc gccaggatgc agaggtaatc atgaagctaa
     6241 ccgatgcgga taatgccgcc gatggcattt ttttccccgc ccttgagcaa aatatgatgg
     6301 gtgcggtgtt aattaacgaa aatgatgaag tgatgttttt caaccccgcc gcagagaagc
     6361 tctgggggta caaacgtgaa gaagtcattg gcaataacat tgatatgctg attccgcggg
     6421 atttgcgtcc tgcgcatcct gaatacattc gtcacaaccg tgaaggcggt aaagcgcgcg
     6481 tcgaggggat gagtcgggag ctgcagctgg agaaaaaaga cggcagtaaa atctggaccc
     6541 gttttgcgct atcgaaagtg agcgccgagg ggaaagttta ttacctggcg ctggtacggg
     6601 atgccagcgt agaaatggcg caaaaagaac agaccaggca attgattatt gccgttgacc
     6661 atctcgaccg accggtgatt gtcctcgatc cggaacgcca tattgtgcag tgcaatcgcg
     6721 catttaccga aatgtttggt tactgcatta gcgaagccag cggtatgcag cctgatacac
     6781 tcctgaacat tcctgaattc cctgccgata accgcattcg tttacaacag ttgctttgga
     6841 aaaccgcccg cgatcaggac gaatttctgc tgttgacgcg caccggtgaa aaaatctgga
     6901 ttaaagcttc tatcagcccg gtttatgacg tgctcgcgca tctgcagaac ctggtaatga
     6961 ccttctcgga tatcaccgaa gaacggcaaa tccgccagct tgaaggcaat attctcgccg
     7021 ccatgtgcag cagcccgcca tttcatgaaa tgggggaaat catttgtcgt aacatcgaat
     7081 ctgttctcaa cgaatcgcat gtttcgctgt tcgcactgcg caacgggatg ccgatacact
     7141 gggcgtcatc ttcgcacggt gcagaagttc aaaatgcgca aagctggtca gcgaccattc
     7201 gtcagcgtga tggcgcgcct gcggggatcc tgcaaattaa aacctcgtca ggagcagaaa
     7261 ccagcgcctt tatcgaacgc gtggcagata tcagccagca tatggccgcg ctggcgctgg
     7321 aacaggaaaa aagccgtcag catattgaac aactcatcca atttgatccg atgaccggtc
     7381 tgccaaatcg caataacctg cacaattacc tcgatgacct ggtcgacaaa gccgtctctc
     7441 cagtatcggt gttgaccata ttcaggatgt gattgatagc cttggctatg cgtgggccga
     7501 tcaggcattg ctggaagtgg tcaatcgctt tcgtgaaaaa ctcaaaccgg atcagtatct
     7561 ctgtcgtatc gaaggtacgc agtttgtcct cgtgagcctg gaaaacgacg tcagtaacat
     7621 tacccagatc gccgatgagc tacggaatgt ggtcagcaag ccgataatga ttgacgataa
     7681 ccccttcccg cttaccttga gtattggcat cagctacgac gtgggtaaaa atcgcgatta
     7741 cctgctctcc actgcccata acgcgatgga ttatattcgc aagaatggcg gtaacggctg
     7801 gcagttcttc agcccggcga tgaacgaaat ggtaaaagag cgtttggttt taggcgcagc
     7861 gctgaaagaa gcgattagca ataaccaact gaaactggtt taccagccgc aaatcttcgc
     7921 agaaacgggt gaactctacg gcatcgaagc ccttgctcgc tggcacgatc cccagcatgg
     7981 tcatgtgccc ccttcacggt ttattcctct cgcagaagag attggtgaaa tcgaaaatat
     8041 tgggcgctgg gtcatcgcgg aagcttgccg tcagttagca gaatggcgta gccagaatat
     8101 tcatatcccg gcgttatccg tgaacttgtc ggcgctgcac tttcgcagta atcagctgcc
     8161 taatcaggtg tctgatgcaa tgcaagcctg gggtattgac ggccaccagc tgacggtaga
     8221 aatcacggaa agcatgatga tggaacacga taccgaaatc tttaagcgca ttcagatcct
     8281 gcgtgatatg ggcgtgggtt tatcggtaga tgattttggc acgggctttt ccggattatc
     8341 ccgcttagtc agtcttccgg taacggaaat caaaattgac aaaagttttg tcgatcgttg
     8401 tctgacggaa aaacgcatcc tggccttact tgaagccatt accagcattg ggcaaagcct
     8461 caatttaacc gtcgtggcgg aaggcgtcga aaccaaagag caatttgaga tgctacgaaa
     8521 gatccactgt cgcgttattc agggatattt cttttcccgc cccctacccg ccgaagaaat
     8581 tccaggctgg atgagcagcg tgttaccgct gaaaatctga caaattcctc tctcccgcac
     8641 tcgcgggttt tcatttaacg tgacactgtc acttaaatac tgtgatttca gctacgattc
     8701 cgggagattc cttccttaac ttcccctctt gcaccaaaac tgcactacgc tgcacaaatg
     8761 ttgatcaaaa gttaaaaaat ataactttaa gccagcattc tggcgcttat cccggcatgg
     8821 catgagatct gcataagcgg aaagcgcagc aatttttgtc ttatacagga accgtttatg
     8881 tcggatacca ccgaactggt tgatttagcc gtgatcttcc ctgatctgga gatcgaattg
     8941 aaatacgcct gcgctgataa catcacaggt aaagctattt accagcaagc gcgttgtctg
     9001 ttacacaagg atgcgattac cgcgctggcg aaaagtatca gtatcgccca gctatcaggc
     9061 ttacaactgg tgatttacga tgcgtatcgc ccacaacaag cacaggcgat gttgtggcaa
     9121 gcctgcccag acccgcaata tgttgttgat gtgacggtcg gttctaatca cagccgtggc
     9181 acggcgatcg acctgacgct tcgtgatgag cacgggaaca tcctggatat gggcgcaggg
     9241 ttcgatgaaa tgcacgagcg ctcccatgcc tatcaccctt ccgtcccgcc cgccgctcag
     9301 cgcaatcgcc tgttgctgaa tgcgataatg actggtggcg gttttgtcgg tatctccagc
     9361 gaatggtggc acttcgaatt accccaggca gcgagttacc ctctgctcgc tgatcaattc
     9421 acctgtttta tatcccctgg cacacagcac gtcagttaat caggaaactg ccatgaagag
     9481 atcgatatcg tttcgtccca cattgctcgc gctcgtcctt gccacaactt tcccggttgc
     9541 gcacgccgcc gtaccgaaag atatgctggt gattggtaag gccgccgatc cacaaaccct
     9601 cgacccggcg gtaacaatag ataataacga ctggacagtg acctacccgt cttatcagcg
     9661 actggttcag tacaaaacgg acggtgataa aggctcaacc gacgttgaag gcgatctggc
     9721 aagtagctgg aaagcgtcta acgatcaaaa agagtggacg ttcaccctga aagataatgc
     9781 taaatttgcc gatggcacac ctgtcactgc cgaagcagta aaactctctt ttgagcggtt
     9841 actaaaaatc ggccaggggc cagcagaagc atttcccaaa gatttaaaga ttgatgctcc
     9901 cgacgaacat acggtgaagt ttacccttag ccagccattt gcaccgttcc tctacacgct
     9961 ggcgaatgac ggtgcatcca ttatcaatcc ggcggtatta aaggaacatg cggcggatga
    10021 tgcccgcggc ttcctcgcgc aaaataccgc cggttccgga ccatttatgc tgaaaagctg
    10081 gcaaaaaggt cagcaattag ttctggtgcc aaatccgcat taccccggca ataaaccgaa
    10141 ctttaagcga gtatcggtaa aaattatcgg tgaaagtgcc tcccgtcgcc tgcagctctc
    10201 ccgtggcgac attgacattg ccgatgcgct gccggtggat caactcaacg ccctgaagca
    10261 ggaaaacaaa gtcaatgtgg cagagtatcc gtcactgcgc gtcacctatc tgtatctgaa
    10321 taacagcaaa gcgccactta atcaggcgga tctgcggcgg gccatttcct ggtctaccga
    10381 ttatcagggc atggttaacg gcattctgag tggtaacgga aaacaaatgc gcggcccgat
    10441 tccggaaggc atgtggggct acgatgcgac ggcaatgcaa tacaaccatg acgaaacgaa
    10501 agccaaagcc gaatgggata aagtgacgag caaacccacc agcctgacgt ttctctactc
    10561 cgataacgat ccgaactggg aaccgatcgc tctggcaaca caatccagcc tcaacaagct
    10621 gggcatcaat gtgaagctgg aaaagctggc gaacgccacc atgcgcgaca gagtgggtaa
    10681 aggtgattac gacattgcga ttggcaactg gagtccggat tttgccgacc cgtatatgtt
    10741 tatgaattac tggtttgagt cagacaaaaa aggtctgccg ggtaaccgct cgttctatga
    10801 aaacagtgag gtcgataagt tactgcgcaa tgcgcttgcg accaccgacc agacgcagcg
    10861 tacccgagac taccagcagg cacagaaaat cgtcattgat gacgctgctt atgtgtacct
    10921 gttccagaaa aactaccaac tggcgatgaa caaagaggtg aaaggctttg tgttcaatcc
    10981 catgctggaa caggtcttca atatcaatac catgagtaaa taacgtgacc ttctggagta
    11041 ttttacgcca acgctgctgg ggctggtgct cgtggtggcg ggcgtctgcg tgattacgtt
    11101 tattatctcg catctgatcc ccggcgatcc ggcgcggtta ctggcgggtg accgcgccag
    11161 cgatgctatc gtggaaaata ttcgccagca actgggactg gaccagccac tgtatgtaca
    11221 gttttaccgc tacgtcagcg acctgtttca tggtgacctg ggaacatcca ttcgtactgg
    11281 gcgtccggtg ctggaagagt tgcgtatatt tttcccggcg acgctggagc tggctttttg
    11341 tgccctgctg ctggcactct tgattggcat cccgctgggc atactctctg cagtctggcg
    11401 aaatcgctgg ctggatcatc tggtgcgaat aatggcgatt accggaatct ccacacctgc
    11461 gttctggctt gggctgggcg tcatcgtgct gttttatggt catctgcaaa tacttcccgg
    11521 cggcggaagg cttgatgact ggctggatcc accaacgcac gttaccggct tttatctgct
    11581 cgatgcgttg cttgaaggca acggtgaagt cttcttcaat gcgttgcaac atctcatctt
    11641 accggcatta acgctggcgt ttgttcacct ggggattgtc gcccgccaga tccgctcagc
    11701 gatgctggaa caattgagtg aagactacat tcgtaccgcc cgggccagcg gcttgcccgg
    11761 ctggtatatc gttctatgtt atgcgctacc caatgcgttg atcccttcga ttaccgtatt
    11821 ggggctggcg ctgggcgatt tgttgtatgg cgcagtgctc accgaaaccg tttttgcctg
    11881 gcccggaatg ggggcatggg tagtgacatc aatacaggcg ctcgacttcc cggcagtgat
    11941 gggctttgcc gtcgtggttt catttgctta tgtgctggtc aacctggtgg tggatttgct
    12001 ctatttgtgg attgatccgc gtatcggacg tggaggtggt gaatgatgct aagcgaggaa
    12061 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
    12121 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gctactgatg
    12181 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
    12241 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttc
    12301 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtcgt ggttgccatt
    12361 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
    12421 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
    12481 gcactggctg ccgctctcgg gccgagtttg tttaacgcca tgctggctat tgctattgtg
    12541 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
    12601 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
    12661 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
    12721 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
    12781 ggcgcgatgg tagcaaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
    12841 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
    12901 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
    12961 aacaactgca tttgagtttc cccggtttta acggcgacgt tcacgcgctc aacaatgtgt
    13021 ccttgcagat taaccgcggt gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
    13081 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
    13141 ggcagatttc actgctggga gacgatgttc ttaacgcgcg ggaaaaacag cttcgtcagt
    13201 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctc aatccgacac
    13261 gtcgaatagg tcttcagatg atggacgtga tccgccatca tcaaccaata agtcgtcggg
    13321 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat accgtggaag
    13381 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
    13441 tggcattctc ctgcgaaccg caattgatta ttgccgacga accgactacg gcgctggacg
    13501 tcacggtaca gttgcaggta ctgcgtttgc ttaaacataa agctcgcgac agtggaactg
    13561 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
    13621 tgatgtatgc cggaagcgtg attgagagcg gcgtgacggc agacgttatc catcatcccc
    13681 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
    13741 cattacccgc cattccaggg acggtaccaa atctcaccca tttgcctgac ggctgcgctt
    13801 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
    13861 gtggtgacaa caaccagcgc tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
    13921 gacacgttat taacgttacg cgacgtccat atcaatttcc cggcgcgtaa aaactggctt
    13981 ggtaaaacta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
    14041 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
    14101 atgggtatgc tgcaaccgag ccacgggcag tacatccgtt ctggctcaca acgcattatg
    14161 cagatgatgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
    14221 atcacagaac cgctctggat agctaagcat agtagtgaac aacagcggcg agcgttggca
    14281 gaggagctgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
    14341 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
    14401 gtgattgtgc ttgatgagcc aacctctgcg ctggatatct ccgtgcaggc gcagatcctc
    14461 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
    14521 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgtg
    14581 gaattagggg aagcgcaaca ggtgctaacg gctccggcac atccatacac ccgattattg
    14641 ctggattccc tccccgccat tgataaacca ctggaggaag aatgggcatt acgtaaaacg
    14701 gatctgccag gaaaccgcac gttgccacag ggctgttttt tccgcgaacg ttgccccctt
    14761 gcaaccagcg gatgcgaagt ccggcaatca ttaacgacgc gggctgacgg acgtgagatc
    14821 cgttgctggc gggcgctgta gagatgctaa caacgcatca ggcattcatg cgcctgacgc
    14881 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
    14941 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
    15001 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
    15061 ccactttatc cagcgacaca tcggcggtgg tatcaatcga tgttggcgtg aatcccgctt
    15121 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
    15181 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
    15241 tcagcacgcc actctcggtg gatactgttc ccttcccgcg tttgatatcg ctttcccagt
    15301 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
    15361 atagactacg tgagaaaccg cacgaggata agcaggatat tccgaataaa atacggcaaa
    15421 aatattactt ctgcacttaa attaaattcc cacaatagat aaccggaaat atatatctca
    15481 agagagattg catcaatatt aatcaactca tcacaataat aatacctatc aatatcatgg
    15541 ggttaaaata taaaaatcat caaccaggac taatcttaac aacgaagcgg caaatatttg
    15601 ccatgctgaa tgtgctttat aaatctgcga tccgtagcag acactataaa tacacagaca
    15661 cggagaatca ctatgtttac ttattatcag gcagaaaatt caacagcaga acccgctctg
    15721 gttaatgcca ttgagcaagg ccttcgggca gagcacggtg ttgtcactga agatgacatt
    15781 ctcatggagc tgaccaaatg ggtggaagct tctgataacg acatcctcag tgatatctac
    15841 cagcaaacga tcaattatgt ggtcagtggc cagcacccta cgctttaaga tgctatgctt
    15901 gatcggcaac ctaatttagg ggtttagcac gtgtttcttc gctacggcga tgttgtcctt
    15961 aaaactagca acaggattga ggagttaaaa tgaaatcgaa ccgtcaggca cgtcatattc
    16021 ttggactgga ccataaaata tctaaccagc gcaaaatagt taccgaaggt gacaaatcca
    16081 gcgtggtaaa taacccaacc ggcagaaaac gccccgctga aaagtaattc ataaccatca
    16141 gtcctcaatg acgattaaac accattgcct gcgcaatggt gtttttgttt ttatctgctt
    16201 tatacttggg gccgacgccc tggcggtaaa gcaaagacga taaaagcccc ccagggatgg
    16261 atattcaaaa aagagtgagt gacatggaac caaaaacaaa aaaacagcgt tcgctttata
    16321 tcccttacgc tggccctgta ctgctggaat ttccgttgtt gaataaaggc agtgccttca
    16381 gcatggaaga acgccgtaac ttcaacctgc tggggttact gccggaagtg gtcgaaacca
    16441 tcgaagaaca agcggaacga gcatggatcc agtatcaggg attcaaaacc gaaatcgaca
    16501 aacacatcta cctgcgtaac atccaggaca ccaacgaaac cctcttctac cgtctggtaa
    16561 acaatcatct tgatgagatg atgcctgtta tttatacccc aaccgtcggc gcagcctgtg
    16621 agcgtttttc tgagatctac cgccgttcac gcggcgtgtt tatctcttac cagaaccgcc
    16681 acaatatgga cgatattctg caaaacgtgc cgaaccataa tattaaagtg attgtggtga
    16741 ctgacggtga acgtattctg gggcttggtg accagggcat cggcgggatg ggcattccga
    16801 tcggtaaact gtcgctctat accgcctgtg gcggcatcag cccggcgtat acccttccgg
    16861 tggtgctgga tgtcggaacg aacaaccaac agctgcttaa cgatccgctg tatatgggct
    16921 ggcgtaatcc gcgtatcact gacgacgaat actatgaatt cgttgatgaa tttatccagg
    16981 ctgtgaaaca acgctggcca gacgtgctgt tgcagtttga agactttgct caaaaaaatg
    17041 cgatgccgtt acttaaccgc tatcgcaatg aaatttgttc ctttaacgat gacattcagg
    17101 gcaccgcggc ggtaacagtc ggcaccctga tcgcagcaag ccgcgcggca ggtggtcagt
    17161 taagcgagaa aaaaatcgtc ttccttggcg caggttcagc gggatgcggc attgccgaaa
    17221 tgatcatcgc ccagacccag cgcgaaggat taagcgagga agcggcgcgg cagaaagtct
    17281 ttatggtcga tcgctttggc ctgctgaccg acaagatgcc gaacctgctg cctttccaga
    17341 ccaaactggt gcagaagcgc gaaaacctca gtgacaggga taccgacagc gatgtgctgt
    17401 cactgctgga tgtggtgcgc aatgtaaaac cagatattct gatcggcatc tcaggacaga
    17461 ccgggctgtt tacggaagag atcatccgtg agatgcataa acactgtccg cgtccgatcg
    17521 tgatgccgct gtccaacccg acgtcacgcg tggaagccac accgcaggac attatcgcct
    17581 ggactgaagg taacgcgctg gtcgccacgg gcagtccgtt taatccagtg gtatggaaag
    17641 ataaaatcta ccctatcgcc cagtgtaaca acgcctttat tttcccgggc atcgggctgg
    17701 gtgttattgc ttccggcgcg tcacgtatca ccgatgagat gctgatgtcg gcaagtgaaa
    17761 cgcttgccca gtattcgccg ctggtcctga acggcgaagg tctggtacta ccggaactaa
    17821 aagatattca gaaagtctcc cgcgcaattg cgtttgcggt tggcaaaatg gcgcagcagc
    17881 aaggcgtggc ggtgaaaacg tctgccgaag ctttgcaaca agccattgac gataatttct
    17941 ggcaagccga ataccgcgac taccgccgta cctccatcta agcctgcgcc cggtagtgaa
    18001 ggctaccggg ctatttccct ctcccttttt cagatctcat ccatactggg tagtggcgaa
    18061 taaatctcat ttgcctcacc tgctatgcag aacatcatcc gaaaaggagg aactatgaag
    18121 gctgcagttg ttacgaagga tcatcatgtt gacgttacgg ataaaacact gcgctcactg
    18181 aaacatggcg aagccctgct gaaaatggag tgttgtggtg tatgtcatac cgatcttcat
    18241 gttaagaatg gcgattttgg tgacaaaacc ggcgtaattc tgggccatga agggattggt
    18301 gtggtggcag aagtgggtcc aggtgtcacc tcattaaaac caggcgatcg tgccagcgtg
    18361 gcgtggttct acgaaggatg cggtcattgc gaatactgta acagtggtaa cgaaacgctc
    18421 tgccgttcag ttaaaaatgc cggatacagc gttgatggcg ggatggcgga agagtgcatc
    18481 gtggtcgccg attacgcggt aaaagtgcca gatggtctgg actcggcggc ggccagcagc
    18541 attacctgtg cgggggtcac cacctacaaa gccgttaagc tgtcaaaaat tcgtcccggg
    18601 cagtggattg ctatctacgg tcttggcggt ctgggtaacc tcgccctgca atacgcgaag
    18661 aatgtcttta acgcgaaagt gatcgccatt gatgtcaatg atgagcagtt aaaactggca
    18721 accgaaatgg gtgcagattt agcgattaac tcacgcaccg aagacgccgc caaaattgtg
    18781 caggagaaaa ccggtggcgc tcacgctgcg gtggtaacag cagtagctaa agctgcgttt
    18841 aactcggcag ttgatgctgt ccgtgcaggc ggtcgtgttg tggctgtcgg tctgccgccg
    18901 gagtctatga gcctggatat cccacgtctt gtgctggatg gcattgaggt ggtcggttcg
    18961 ctggtcggca cgcgccagga tctaactgaa gccttccagt ttgccgccga aggtaaagtg
    19021 gtgccgaaag tcgccctgcg tccgttagcg gacatcaaca ccatctttac cgagatggaa
    19081 gaaggcaaaa tccgtggccg tatggtgatt gatttccgcc gctaagaggc ctttgctgcg
    19141 actcccatgt tcgggtcgca gcatcgcaca ctctccaaca tgaaatggct aaatggatta
    19201 ttgttgcgta ggaaggaaca ataatgatag tgttcccaga gaaggaacgc tactgagagt
    19261 aaagacacta atgaaaatgg ccaatcatcc ccgcccgggg gacattattc aggaatcact
    19321 ggacgaactt aatgtcagcc tgcgcgagtt tgccagagca atggaaattg ctccctcaac
    19381 ggcaagccga ttgctgaccg gaaaagcagc tttgacgcca gaaatggcaa ttaaactttc
    19441 cgtggtgatc ggcagttcgc cgcaaatgtg gctgaatctg caaaacgcct ggagtctggc
    19501 agaggctgca aaaacggtgg atgtgtcgcg attacgccgt ctggttgcgc aataaaaaat
    19561 ggcgcctttt acagcgccat ttgaaactga tgacaaacgc aacattgcct gatgcgctac
    19621 gcttatcagg cctacacgtt cactgcaatc tattggattt gaacggtttg taggccggat
    19681 aaggcgttca cgccgcatcc ggcatgaaca aagcctacgt tgtctacaat atgaaaatgg
    19741 cgccttttac agcgccattt tcagcttaaa gttcaaaaga gattatatcc cttcttcact
    19801 ctcttttttc gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc
    19861 ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc gatcctttca cccagaatgc
    19921 catatacata tggatcagga tggcgtggat caggatgata cccgcagccg cgtggatcag
    19981 caggctgtag cgaacaacct gcatcgggaa gtactgcgca aagtacggac gccagataat
    20041 caccccggtc accagcagca cgaaaatcat gctcatgatc gaccagaaca tcatcttttg
    20101 cccggcgttg tacttaccga catccgccac tttatgctca ttgcctttca atacttcgac
    20161 aatgtttaac agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac
    20221 aaacataaac atcagtgcga cgaaaatcgc aatgccgaag aatgggtgca aaatgcgtcc
    20281 catctgcggc gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat
    20341 cccggacagc gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat
    20401 aaatttggtg cgcacaatca ttttcgactt actcatgatg atcctcctcg tcatcgtcca
    20461 cttccttatt cgggccaata ccgatgtagt ggaaaatcaa ccctgcaaaa gtggcaataa
    20521 agccagccgc tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct
    20581 tcggatcttt cggcagaccg tgatacagct ccggctgatc ggcgtgatgc agcacgtaca
    20641 taacgtgcgt accaccgacc ccttccgggt tgtagacgcc agcatgttcg taaccacgcg
    20701 ctttcagttt cgccacgcgc tgttccgcca gctccagcat ctccttcttg gtgccgaagt
    20761 ggatagcccc ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat
    20821 cgacgcagag cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa
    20881 acggacaccc ggcaatgcag taaccacagc cgatgcagtt ttccgactgg aaatcgacaa
    20941 tcccgttagc gtactgaatg attgcaccgg cagacgggca cgccttcagg cagcccggat
    21001 cttcacagtg catacagcca tctttacgga tcagccactc cagcttgccg ctctgttcgg
    21061 tttcgctaaa gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa
    21121 ccccgacgca gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac
    21181 aggctttaca gccgatacag gtggaaacgt cgataagttt tgcgacttct gctttgtaat
    21241 cacgcacctg agaaggcggc gtgatggagt tagttgcgga ccttttgata atgtcctgcg
    21301 tttccatagc catctgttcg cccccttacg ccttctcgat gttgactaag aacgctttat
    21361 attccggcgt ttgcgagttt gcatcaccga cattcggcgt cagagtgtta gctatataac
    21421 ctttacgcgc gacaccctca aagccccagt ggattggaat acccaccgtt tcaacctgct
    21481 gaccatttac attcagcggt ttcagacgac gcgttaccac agccaccgcg cggataaagc
    21541 cacgcttgct tgagacagtg acacgatcgc cattattaat gcctttcgcc gccgccagcg
    21601 tttcgctgat ttccacaaac tgttccggct gagcaattgc gttgagcaac gcgtgcttgg
    21661 tccaggtgtg gaagtgctcg gtcagacgat aggtcgtacc cacatacggg aactgctctt
    21721 ttttacccat ccgcagcgcg tcttgttcat acagacgaac caccgggtta gaaaccacgt
    21781 tcggatgcag cgggttagtt cccagcggcg tttcaattgg ctcgtagtgt tccgggaacg
    21841 gaccttccgc cattttgttg atggcaaaca ggcgtcccat cccttccggc tgcatgataa
    21901 acggcccggt tggcgtaccc ggtgcggcat tgccgaagtc agggatatcg ttacccgtcc
    21961 acttgctgcc gttccactgg atcagcatcc gtttcggatc ccacggttta ccgttgatat
    22021 ctgccgaagc acggttgtag agtacgcgac ggttaagcgg ccacgcccag gcccatccca
    22081 gcgtattccc cagaccggac gggtcggagt tatcgcggtt agccatctgg ttgccctgct
    22141 ctgtccagct accggtgtag atccagcaag aagatgcggt tgtaccgtca tcacgcagat
    22201 gcgcaaagct actcagcaac tgacctttct tcgcaatcag cacgccatta gcatcataga
    22261 gatcttccag cgcatagccg ttgttctctt tagctacttc gtcagattgc ggttcgtgcg
    22321 gctgcttgta gttccagctc atcttcatca gcggttctac gcctttacca ccttcagcct
    22381 ggtacagctc gcgcagatga tggtagatac ccgccagaat ttcgccgtcg ttacgcgctt
    22441 cgcccggcgc gtcctgacct ttccagtgcc actgcaacca gcgaccggag ttagcgatag
    22501 aaccatcttc ttctgcaaag caggtcgaag gcagacggaa tacttcagtc tgaatagacg
    22561 ccggatcgac atcgttcgac tcgccgtggt tctgccagaa ggtagaggtt tcagtcacca
    22621 gcggatcgat aaccaccatg tacttcagct tgctcaggca gctcaccact ttgtttttgt
    22681 ccgggaagga cgcaaccggg ttaaagccct ggcagaaata accggtgact ttgccttcat
    22741 ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc ccacttcggc agccagtcat
    22801 agccccagtt gttctctttc tgcgcggcat cgccatagaa agatttcatc aggctaacga
    22861 agaacttcgg atagttgctc cagtagttca cctgaccagc cagcgtcgct ttcggcgtgt
    22921 tcgcttccag atacgactgc aaatcaacct gtttttctga cggcagcgtc agataacctg
    22981 gcaggctggt agagagcaga cctaagtcag tcagaccctg aatgttggag tgaccacgca
    23041 atgcattcac gccgccaccg gccataccca tgttaccgag cagcaactgg atcatcgcca
    23101 tagtacggat gttctgcgca cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg
    23161 tggttgtgcg atccggtgcg ctggtggagg ccagcacttc acacactttc aggaagtcgg
    23221 cttttggcgt accacagatg ttttcaacaa cgtccggcgt gtagcgggaa acgtgctctt
    23281 tcagcaggtt ccacacacag cgcggatgag tcagtgtttc atcgcgtttt gcatagccgt
    23341 tttcatcgaa ctgatagttc caggacgact tatcgtactg gcgtttttca gcgtcgtagc
    23401 cgctgaacag accgtcttcg aaagcaaaat catcacgcac cagcaggctg gcgttggtgt
    23461 aatgcttaac gtattcggcg ttgattttgt tgttttcgat caggtagcgc aaaacgccag
    23521 acaggaacgt aatgtccgta ccggaacgaa taggcgcgta gatatccgcc acagaagcgg
    23581 tacgcgtaaa acggggatcg acaacgatca aggttgcatc gttgttgttt ttcgcttcca
    23641 tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt accgcccatt accattacga
    23701 cgttagcgtt tttgatatcc acccagtggt tggtcatcgc accgcgacca aatgttggag
    23761 caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc
    23821 cgagggagcg ggcaaatttc tgggtcagca tcccggtttc gttgctggca ccggaagcac
    23881 acagcatacc ggtagaaagc caacggttta ccgttacgcc ctgctcgttc ttttcaataa
    23941 agttagcgtc acggtcagct ttcatcagct tcgcaatacg ggagaatgct tcttcccagc
    24001 taatgcgctg ccatttgtca gaacctggcg cacgatattc cgggtagcgc agacggtttt
    24061 cgctgttgac gtaatccagc aaaccggccc ctttcgggca cagcgcccca cggcttaccg
    24121 gatgatccgg gtcaccttca atgtgataaa tcgcttctct ggcgtttttc gcgccatcac
    24181 ccaggctata catcaatagc ccgcaaccta cggaacagta tgtgcaggtg ttacggatct
    24241 ctttagcgcg taataatttg tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc
    24301 ccaatgccgc tactgttgtt ccagccatac cgcccgcgca gattttaaaa aattgtctgc
    24361 gactgacgtc cattgctttc ctcttttttc aggggtatta ctgcgcgagg aaactaacag
    24421 taaaattctg aatctttttg cgcgaaatca aaaagaacac gtgtttgcca caaaataatc
    24481 cgcctgcgca aattgaccta cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc
    24541 cagttaaaaa aatgctataa aattcagctt aatttttaac ggcaagagag acaaaacagc
    24601 gagcatgaca cgacaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac
    24661 gatggtagga ttgattcgcg gtgtcagtga ggggctcggc ccggtcggtg gcgcagctgc
    24721 tatctattca ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattcggca
    24781 aatcccgaaa ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct
    24841 ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg attgaagtgg gtatggtgaa
    24901 ctatctgtgg cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa
    24961 ctggctgatt gtaccaggat tattattagc cctcgtcggc gtctgctggg tgttaggcgg
    25021 tgacaatggg ttacattatg atgaaatcat caataatatc accaccagcc cattgagtta
    25081 tttcctggcg ttcattggtg cgtttatctg ggcagcctac tgcacagtaa cgaataaata
    25141 cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta acgggagcaa gtctgtgggt
    25201 ttactatttt ctcacgccac aaccagaaat ggtatttagc acgccagtca tgattaaact
    25261 catctctgcg gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatactgca
    25321 tggcaacgtc accataatgg cggtaggttc gtattttacg cccgtacttt cctcagcgct
    25381 tgcagccgtg ctgctcagcg ccccgctgtc gttctcgttc tggcaaggcg cgctgatggt
    25441 ctgcggcggt tccctgctct gctggctggc gacacgtcgt ggctaaaata attgccgggt
    25501 ctacggcccg gcaattaatc agataaaccc cgacaatatt aaaaaataaa tttcaaatac
    25561 cagtaattca ccttatatct catttcgact ccgatcacat atcgataaat aatattattt
    25621 catctatttt tctcattgtt tttaatcacc attctaattt aaaaacagta tgtcatttat
    25681 aaatggcgcc gtttaaaatt cgtttaaaaa atacgtttaa cgataattaa aaggattctg
    25741 ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc
    25801 acacgctgcc gaagtctata acaaggatgg taataaactc gacctttatg gcaaggttac
    25861 cgctctacgt tattttactg atgataagcg tgacgatggt gataaaactt atgcccgtct
    25921 cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt ggttttggtc actgggaata
    25981 tgattttaaa ggctataacg atgaagccaa cggctcgcgc gacaacaaga cccgtctggc
    26041 ctatgctggt ttaaaaatta gtgaatttgg ctctctggac tatggccgta actacggtgt
    26101 cggctatgac attggttcat ggactgatat gttgccagaa tttggtggcg atacctggag
    26161 tcagaaagat gtcttcatga cataccgtac caccggtgta gcaacctatc gcaactacga
    26221 tttctttggc ttaattgaag ggctgaactt tgccgcgcaa tatcaaggca aaaatgaacg
    26281 tactgacaac agtcatcttt atggtgctga ctacacgcgt gccaacggtg acggtttcgg
    26341 tatctcctca acttatgttt atgatggctt tggtatcgga gcggtgtata ccaaatccga
    26401 tcggacaaat gcgcaggaaa gagccgctgc taatcctctc aatgcctccg gtaagaatgc
    26461 agaactgtgg gctacaggta taaaatatga tgccaacaac atctactttg cagctaatta
    26521 cgctgaaaca ttaaacatga ccacctatgg cgatggttat atttctaaca aagcacaaag
    26581 ttttgaagtg gtggcgcaat atcaattcga cttcggcttg cgcccctcac tcgcttacct
    26641 gaaatcgaaa ggcatagatc tgggccgcta cggcgatcag gacatgattg agtatatcga
    26701 cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa
    26761 cctgattgat gaaagcgact ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc
    26821 tacgggcatt acctatcagt tctaacatcc tctcctgacc agcccttctc tgggctggtt
    26881 tttgatttaa gtgctgctgt atgactcctt aagtaaaaat gagcaatttt tgatatttct
    26941 ggtcattcac ctcaattaat attattccat a
//
LOCUS       DAZKIF010000061        26739 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZKIF010000061 DAZKIF010000000
VERSION     DAZKIF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.061"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..843
                     /locus_tag="ACHOTW_002509"
     CDS             <1..843
                     /locus_tag="ACHOTW_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844596.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAWCQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAM
                     ARIK"
     gene            979..1662
                     /locus_tag="ACHOTW_002510"
     CDS             979..1662
                     /locus_tag="ACHOTW_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844597.1"
                     /translation="MKLPVKLLMSLISLVRVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            1678..2088
                     /locus_tag="ACHOTW_002511"
     CDS             1678..2088
                     /locus_tag="ACHOTW_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844598.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            2309..3130
                     /locus_tag="ACHOTW_002512"
     CDS             2309..3130
                     /locus_tag="ACHOTW_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9844599.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            3212..3691
                     /locus_tag="ACHOTW_002513"
     CDS             3212..3691
                     /locus_tag="ACHOTW_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9844600.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSAIMRIID"
     gene            3706..4182
                     /gene="yeeS"
                     /locus_tag="ACHOTW_002514"
     CDS             3706..4182
                     /gene="yeeS"
                     /locus_tag="ACHOTW_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9844601.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            4245..4466
                     /gene="yeeT"
                     /locus_tag="ACHOTW_002515"
     CDS             4245..4466
                     /gene="yeeT"
                     /locus_tag="ACHOTW_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9844602.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            4540..4908
                     /gene="cbeA"
                     /locus_tag="ACHOTW_002516"
     CDS             4540..4908
                     /gene="cbeA"
                     /locus_tag="ACHOTW_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFP9844603.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            4997..5370
                     /gene="cbtA"
                     /locus_tag="ACHOTW_002517"
                     /pseudo
     CDS             4997..5370
                     /gene="cbtA"
                     /locus_tag="ACHOTW_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            5367..5561
                     /gene="yeeW"
                     /locus_tag="ACHOTW_002518"
     CDS             5367..5561
                     /gene="yeeW"
                     /locus_tag="ACHOTW_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9844604.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            5607..5687
                     /locus_tag="ACHOTW_002519"
                     /pseudo
     CDS             5607..5687
                     /locus_tag="ACHOTW_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5976..6122)
                     /locus_tag="ACHOTW_002520"
                     /pseudo
     CDS             complement(5976..6122)
                     /locus_tag="ACHOTW_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            6176..6358
                     /locus_tag="ACHOTW_002521"
     CDS             6176..6358
                     /locus_tag="ACHOTW_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFP9844605.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(6459..6788)
                     /gene="tmaR"
                     /locus_tag="ACHOTW_002522"
     CDS             complement(6459..6788)
                     /gene="tmaR"
                     /locus_tag="ACHOTW_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP9844606.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6960..8018)
                     /gene="yeeA"
                     /locus_tag="ACHOTW_002523"
     CDS             complement(6960..8018)
                     /gene="yeeA"
                     /locus_tag="ACHOTW_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9844607.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(8217..8690)
                     /gene="sbmC"
                     /locus_tag="ACHOTW_002524"
     CDS             complement(8217..8690)
                     /gene="sbmC"
                     /locus_tag="ACHOTW_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP9844608.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(8809..9975)
                     /gene="dacD"
                     /locus_tag="ACHOTW_002525"
     CDS             complement(8809..9975)
                     /gene="dacD"
                     /locus_tag="ACHOTW_002525"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP9844609.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            10184..11611
                     /gene="sbcB"
                     /locus_tag="ACHOTW_002526"
     CDS             10184..11611
                     /gene="sbcB"
                     /locus_tag="ACHOTW_002526"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP9844610.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(11654..11881)
                     /gene="tsuB"
                     /locus_tag="ACHOTW_002527"
     CDS             complement(11654..11881)
                     /gene="tsuB"
                     /locus_tag="ACHOTW_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFP9844611.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(11895..12953)
                     /gene="tsuA"
                     /locus_tag="ACHOTW_002528"
     CDS             complement(11895..12953)
                     /gene="tsuA"
                     /locus_tag="ACHOTW_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFP9844612.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(13132..14490)
                     /gene="plaP"
                     /locus_tag="ACHOTW_002529"
     CDS             complement(13132..14490)
                     /gene="plaP"
                     /locus_tag="ACHOTW_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP9844613.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(14480..14542)
                     /gene="yoeI"
                     /locus_tag="ACHOTW_002530"
     CDS             complement(14480..14542)
                     /gene="yoeI"
                     /locus_tag="ACHOTW_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP9844614.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(14757..15686)
                     /gene="yeeY"
                     /locus_tag="ACHOTW_002531"
     CDS             complement(14757..15686)
                     /gene="yeeY"
                     /locus_tag="ACHOTW_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9844615.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHNVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(15732..16556)
                     /gene="yeeZ"
                     /locus_tag="ACHOTW_002532"
     CDS             complement(15732..16556)
                     /gene="yeeZ"
                     /locus_tag="ACHOTW_002532"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9844616.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            16982..17032
                     /gene="hisL"
                     /locus_tag="ACHOTW_002533"
     CDS             16982..17032
                     /gene="hisL"
                     /locus_tag="ACHOTW_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP9844617.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    17008..17131
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            17178..18077
                     /gene="hisG"
                     /locus_tag="ACHOTW_002534"
     CDS             17178..18077
                     /gene="hisG"
                     /locus_tag="ACHOTW_002534"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP9844618.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            18083..19387
                     /gene="hisD"
                     /locus_tag="ACHOTW_002535"
     CDS             18083..19387
                     /gene="hisD"
                     /locus_tag="ACHOTW_002535"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP9844619.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            19384..20454
                     /gene="hisC"
                     /locus_tag="ACHOTW_002536"
     CDS             19384..20454
                     /gene="hisC"
                     /locus_tag="ACHOTW_002536"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP9844620.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            20454..21521
                     /gene="hisB"
                     /locus_tag="ACHOTW_002537"
     CDS             20454..21521
                     /gene="hisB"
                     /locus_tag="ACHOTW_002537"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP9844621.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            21521..22111
                     /gene="hisH"
                     /locus_tag="ACHOTW_002538"
     CDS             21521..22111
                     /gene="hisH"
                     /locus_tag="ACHOTW_002538"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP9844622.1"
                     /translation="MNVVILGTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            22111..22848
                     /gene="hisA"
                     /locus_tag="ACHOTW_002539"
     CDS             22111..22848
                     /gene="hisA"
                     /locus_tag="ACHOTW_002539"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP9844623.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            22830..23606
                     /gene="hisF"
                     /locus_tag="ACHOTW_002540"
     CDS             22830..23606
                     /gene="hisF"
                     /locus_tag="ACHOTW_002540"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP9844624.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            23600..24211
                     /gene="hisIE"
                     /locus_tag="ACHOTW_002541"
     CDS             23600..24211
                     /gene="hisIE"
                     /locus_tag="ACHOTW_002541"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP9844625.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(24308..25285)
                     /gene="wzzB"
                     /locus_tag="ACHOTW_002542"
     CDS             complement(24308..25285)
                     /gene="wzzB"
                     /locus_tag="ACHOTW_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP9844626.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(25431..26597)
                     /gene="ugd"
                     /locus_tag="ACHOTW_002543"
     CDS             complement(25431..26597)
                     /gene="ugd"
                     /locus_tag="ACHOTW_002543"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFP9844627.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
BASE COUNT         6900 a         6891 c         6611 g         6337 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatatgca gggaaatacc
      481 cctgtgacgc ctctggtcga tgccggatgc aatatggtga ttgtgacgca tctgagtgac
      541 ggttctttat gggatcgcca ggcttttccg gacaccacaa tccttgagat ccgtccccgg
      601 aaaaggctga aatatgcagg tgatggtggc aacagcggcg gtctgctcag ttttacatcg
      661 gcacataccg atgcctggtg tcagcagggc tatgaagaca cgatgctggc gatggagcat
      721 atccggaaac cgctggcagc acgtcaggca ctgacccggt cagaggcggt attgcagaaa
      781 agcctggata taacggaaga ggcagatttg gcactgagaa acgcgatggc ccggattaaa
      841 taaagacgcc ccaaagaaac gccaccgcct gtggtggtgt ttttcctgtc tggtgttatt
      901 gaaaatattt tcagatatat attacctgag agaaagtttt acccctcacc tgtcaattca
      961 ctcactggaa atttaattat gaaattacct gtaaagttat taatgagcct tatatctctg
     1021 gtccgcgtta ttgcacgtgc cggggaatat aaaaattaca gccgggatga aataaaatac
     1081 tggcgatata catcatacaa gggggggaaa cttccggaag gtttcactga tgagaaattt
     1141 tccagcgcca tttacaacgg aagaatattt acaatgaaac gtttacatac ccttatgtta
     1201 tttctggcgg ttctgttcac gggctttaac tttaacgcag aagcggctac cgtaaaacaa
     1261 gctcttagtt gtaacccgga agcctgggct gaacaacctg gagcgtgtcc atcaacgtac
     1321 gagttgtacg aaggtgacgc tacctacaaa gccgctatcg acaaagcatt aaaaccggtc
     1381 ggactgagcg gcatgttcgg taaaggcggc tatatggatg gccctggtgg aggtatcacg
     1441 ccagtaaaca ttaacggtac agtctggttc cagggcgacg gttgcaaagc caatacctgc
     1501 ggctgggact ttatcgtaac actctataac ccaaaaaccc atgaagttgt tggctaccgt
     1561 tactttggtt tagatgaccc ggcctacctg gtttggttcg gtgaaattgg cgtgcatgaa
     1621 ttcgcgtatc tggtgaaaaa ctacgtagct gcggttaact aatttaagga gtctatcatg
     1681 aaaactttta tcaaaacttt actcgttgct gtaactattc tgttctctgt cttcgctact
     1741 gcgaaacaag taaaactgcc aaataacatc aaatacgtta atactacaga agcgttttcc
     1801 tgtaccgaaa ttgacggtat gaattgccag acaaagaatc agttcaacta taaagataac
     1861 agctatgttt tcgtgcttga acgtggtggt gcctggtgct acgactacac tgtctcggta
     1921 gttaacctga aaaccgggaa agcacaaatg attgaatacg gggacaatca actgtgctca
     1981 ggtagcaaca aaccgttctt cgaaatcaaa aatggcgtac cgacggtagg agtcatcgac
     2041 acatccggaa aacctgtcgg tgtagcccag gacaaactta aaatctaagg agtaaaccat
     2101 gcgtttctct gcacattatt aatatcaaag gtaaatacag ccccagccat tgttaccgga
     2161 aagccgctta atgcgtggct cgccagtgaa aggcctctca gacaataaac cttattcatt
     2221 tcacccgtca ggccgcgtct tctccgggag acgcggcttt tttcatttat tgcaccagta
     2281 aatcttaacc accgataagg agcaaagtat gcgattagcc agtcgttttg gttatgctgc
     2341 aaaccagata cgccgtgacc gtccgctgac acatgaagaa ctgatacgcc atgtacccag
     2401 tatttttggg gaagaccggc acacctcccg cagtgaacgg tatgcgtaca ttcccaccat
     2461 taccgtcctg gaaaatctgc agcgggaagg ctttcagccg ttcttcgcct gccagacccg
     2521 tgtgcgcgac ccgggccgcc ggggatacac aaaacacatg ctgcgtctgc ggcgggccgg
     2581 agagataaac ggagaacatg tccctgaaat tattctgctc aactctcatg acggtacctc
     2641 cagctaccag atgctgccgg gttacttcag gttcgtctgc cagaacgggt gcgtctgtgg
     2701 ccagtctctg ggggaagtgc gtgttccaca ccggggaaat gtagtggaga aagttatcga
     2761 aggggcttac gaggtggtgg gcgtgtttga ccggatagag gagaagcgtg atgccatgca
     2821 gtcgctggtc ctgccgcaac cggcacgcca ggcgctggca caggcggcac tgacttaccg
     2881 ttatggtgac gaacatcagc ccgtcaccac cgccgacatt ctgacgccgc gacgccggga
     2941 ggattacggt aaggacctgt ggagcgcata tcagaccatc caggagaata tgctgaaagg
     3001 cgggatttcc ggtcgcagtg caaaaggaaa acgtatccat acccgggcca ttcacagcat
     3061 tgataccgac attaagctca atcgcgcatt gtgggtgatg gcagaaacga tgctggagag
     3121 cctgcgctga tgccgtttcc ctgagtgagg gaatcattca gctttcgttc ccgaaaaaca
     3181 cacatttatt tgttatttaa ggagttatct catgaaaacc gtttctcaga atacccccac
     3241 aatttattca gctacaacac cagagaataa tccgcctcag ttggttgcca gcctcgtccc
     3301 tgatgaacag cgcatcagct tctggccgca gcattttggc ctcattccac agtgggtcac
     3361 cctggagccc cgtgtcttcg gctggatgga ccgtctgtgc gaagactact gcggtggtat
     3421 ctggaatctg tacaccctga acaacggcgg ggcatttatg gcccccgaac cggatgatga
     3481 tgatgacgaa acatgggtac tgttcaatgc catgaacggt aaccgcgctg aaatgagccc
     3541 ggaagccgcc ggtattgccg cctgtctgat gacgtacagc catcatgcct gtcgtacgga
     3601 gaattatgcc atgacggtcc attattaccg gttgcgggat tacgccctgc agcatccgga
     3661 aggcagcgcc attatgcgca tcattgactg aaaggggccg gaataatgca acagatttcc
     3721 tttctgcccg gagaaatgac gcccggcgag cgcagcctca ttcaacgggc cctgaaaacc
     3781 ctggaccgcc atcttcatga acccggtgtg gccttcacct ccacccgtgc agcacgggaa
     3841 tggctgattc tgaacatggc gggactggag cgtgaagagt tccgggtgct gtatctgaac
     3901 aaccagaatc agctgattgc cggtgaaacc ctcttcaccg gcaccatcaa ccgcacggaa
     3961 gtccatcccc gggaagtgat taaacgcgcc ctgtaccaca atgccgctgc cgtggtactg
     4021 gcacacaatc acccgtccgg tgaagtcaca cccagtaagg cagaccggct tatcaccgaa
     4081 cgtctggtac aggcactggg cctggtggat atccgggtgc cggaccatct gatagtcggt
     4141 ggcaaccagg ttttctcctt tgccgaacat ggtctgcttt aacccgtcac aaccacatca
     4201 cacctgtttt cacttttatc ttctgtcttc agaggtatcc cattatgaaa attatcaccc
     4261 gtggtgaagc catgcgtatt caccaacaac atccgacatc ccgtcttttt ccgttctgta
     4321 ccggtaaata ccgctggcac ggcagtgctg aagcgtatac cggtcgtgaa gtgcaggata
     4381 ttcccggtgt gctggccgtg tttgctgaac gccgtaagga cagttttggt ccgtatgtcc
     4441 ggctgatgag cgtcaccctg aactgagtgg gaattctgat gagcagaatt atcgccacta
     4501 ccggaccatt cttagccgat tttctgtaag gattttatcg tgtcagacac actccccggg
     4561 acaacacttc ccgacgacaa tcacgaccgc ccctggtggg ggctgccctg caccgtgacg
     4621 ccctgtttcg gggcacgtct ggtgcaggag ggtaaccggt tgcattacct tgccgaccgc
     4681 gccggtatca gaggcctgtt cagcgatgca gatgcgtacc acccggacca ggcctttccg
     4741 ctgctgatga aacaactgga actcatgctc accagcggtg aactgaatcc ccgccatcag
     4801 cataccgtca cgctgtatgc aaaagggctg acctgcaaag ccgataccct cagcagttgt
     4861 ggttacgttt atctggctgt ttatccgacg cccgaaatga aaaattaact ctccagaata
     4921 gccttctgct acggcctggt gttttcacca cgccactttt ccatttttat atctgcatat
     4981 caggaaaatc ttcagtatga aaacattacc tgtattaccc gggcaggcgg ccagttctcg
     5041 cccgtctcct gttgaaatct ggcagatact gctgtcccga ctgctggacc agcactatgg
     5101 cctcacactg aatgacacac cttttgccga tgaacgtgtg attgagcagc atattgaggc
     5161 aggcatttca ctgtgtgatg cggtgaactt tctcgtggaa aaatacgcgc tggtgctacc
     5221 gaccagccgg gattcagcgc ctgtacccgc tctcagttaa taaacagcat cgatatcctc
     5281 cgggctcgca gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt
     5341 accctgggta agtatccgga ggcgaaatga tgacactgga agccgacagc gttaacgtac
     5401 aggcgctgga tatggggcac attgtcgttg acattgatgg tgttaatatc actgaactga
     5461 ttaataaggc cgctgaaaac ggttattcac tccgcgtggt ggatgaccgt gactctaccg
     5521 aaacaccggc aacttatgcc agccctcacc agttgctgta agacaatgca gtgatgcagg
     5581 gtatagtgcc attaaccaca gaaacagcac tgttgtcctg tattaagcag gcaagagaag
     5641 atgcccttac cctgcgccat ctgcagcttc tgcaccagaa ccggtgaatc tcactcaaca
     5701 ggcttcattt cccctgacgc cagcctgaat acagctggcg ttttcattta taaacagaaa
     5761 ggaaaaccga taataatgga atggacgccc cgaccatgaa gcaacggtag gatattactg
     5821 ataaaccttc cagtggaggt gcgttatggc cgttttaacg attagcattg acctgacaaa
     5881 aaatgaattc caaatccacg gtctgggtag gaacagaaaa atctaagctc agaaaacgga
     5941 ttaaagtaag cgtcaacgga gcaccgtatt gacgcttatt tattggtgag aactacgttc
     6001 catggcagga gttcgtcaac acggttggag ggccattccg gcagtatgct cagaatatgg
     6061 cgcagatacg cttccggatc gataccgttc agacggcagg tgcctgcttt tccggcaaca
     6121 agcaggaatc tcagcgctga agcaggaatt agaggtaaaa acgccctacc gggccatgaa
     6181 tcacccggtc attggagtag taaccaaagc agatttagcc agcatggaac aaatatcttt
     6241 ggtgaaatgt tggttacggg aagctggagc gcacaacgtg ttagtaacca gtgcggttaa
     6301 taacaatgga gttacagaac tttttgcctt gctgcataca gaagaaggct gccgctaatt
     6361 tcgataggtt ctcagatgag aatgatgacg gtgatacaca gataagaatc aatgagcaat
     6421 gagggttgcc gggcaaccct cattgaataa aacaggaatt acttcgcttc gccgtttttc
     6481 atttcgccca tagctttcag ctttttggag atatcgcggc gttctttgga gagctcggca
     6541 tttttgatga tgtaatcgtc aacgcgatct tcatagtcac ctttcatgct ggcgatgatg
     6601 ccctggattg cttcaacgct catccctggc ttgatgtaat cgctcaggtt gtccagcaac
     6661 aggacgcgct tctggttgtc acggatcttt ttctcaacgt cctgaatttc acgttgcagt
     6721 ttgttcttac gacggaacag acgaacaaat tccagtacgt cctggaatga aggcttggta
     6781 gtttccattt ttatacccct gataatgtga gagtcggatt cgtttaagca accgctattc
     6841 gttagggcca acattactca tagctgccgc agatgacaat gcttttatcc cttatcacta
     6901 tcataccctt tatccttgct gaatcgaagc agcagcaaga tgattctgaa gttcaggaat
     6961 tatttgcgca aggcccggca aatcagactc gatagcaact caagctgatg tgccgtttcc
     7021 atgttcagcc acacataacc atagattggt gtttccacaa ccttcaggtc atggtggtta
     7081 ttcagcaact gacgcagctc ttccacagca tcgttcaatt tttccgtatt ggcaaaaacc
     7141 ggctgcggat taccttcgta cagcgcatga acaaggctca gcagtatttg ctgcatcatg
     7201 tgctgggtat cacgaagttt ttgcgcgttc aataacacga aatggctggg gcgcgtggcc
     7261 cagtatgcat tgatttgcaa ctccagcata caaaccagat tgcggttaat ggtctggata
     7321 ccttcatata tcgattttgg aatacgagtt tctttgctgg cgggcgcaat cagcccacgc
     7381 attttcacgg catcggtcag gagtttttgt agatggcttt ccagacgtgg gcgttcgagt
     7441 aagttcggtg agaatgcaga ttgatagacc cgattatact cggtcagact tttcgccagt
     7501 tgaatgcgcc agtggatgaa cgcccgttgt ggccagatac cggtaaacaa cattgccagt
     7561 aaagagccga ggatcacatc gccgcttcgc cataacgccg tatcaatttc acctgtcggg
     7621 gaacccacaa caattgccag cgtcacccca atcaataatc cttgatacgg tttcttgccc
     7681 agcgccagcc aaccgcaaag aaacatcgcc gccgcgcacc agactaacat cagcggtaac
     7741 gaaattaact ccagttgcag agcgataaga cctaaaatcg accccaacac cgtaccgcca
     7801 atacgttcga aggcacgagg gacaacgttc ccccagaacg agattggccc cataatcacc
     7861 accatggtga ccagcggcca ggtgccttcc gggatagtaa acaggcggat aatgagaaaa
     7921 gtgagcagga atgccagcgc gacccgagta ccatgcacaa tgcggtaatg gcggtatacc
     7981 cggatttcaa acgggcttaa tgacttattg gcacgcacgc agaaactccg ttaatgaaga
     8041 ttaaaagcgc tcataacaca tctgtcggtc agaaggcgcg cctctgaatt atccgacgga
     8101 caaaccggga acagtgtaat ttcccggcag gagcgaaaaa attgaaaggc gcatcttact
     8161 cttttcgctt tcaaaaaaag agtggtcttc gcgtcaacac accgccctga gatgaattag
     8221 tgatgttttg gctgcaccgc aacatacatt tcgatatccc agtacccatc ttccgcgcca
     8281 ttgttcaagt aaacctcgaa gcagggcttt ggtaacattt cataagcact gtcctgcaag
     8341 aggctattaa agaactgata ccagggttta gcaaaatcat caccgactac acgagccacc
     8401 gccaccgcat attgaccacc tgaaatttct gtcagaatga cgccctcact gttttcgggg
     8461 agcgtaaagt tattcggtac cgtcacgacg gtgtcgcagc gtaatttttc ggcgggtgtt
     8521 tcatccggat tgtcgtaata gacagcaacc cactccttcg gcacaatatt tttgctatct
     8581 acccacatca tcaactgctc aaaacctttc tttaccgtct gttcccacgg gccaacgaga
     8641 tggaaacctg caacggtacg tttatcttcc tgcttaatct cgtagttcat gacgcctcca
     8701 ttattactgt ttttatatac agtatagttg caatattaaa accacaagga atgagtgttg
     8761 attatgcgag cagactcgca ctcctgccag tctgctgcaa aagaaaggtc aggccttatg
     8821 gtggaaataa tcactcaggc gagaaaacat gctgccttcc ccgacagatt ccagggtaac
     8881 cagcggccag tgcgccacct gtttatcacg gtcgtaaagt tcaatttccc ctacccgctg
     8941 atgggcgcta attggcgcgg tgagctcttt accatcaagg gtatatttgg ctttgatatg
     9001 tggaatttcg gctttcggta gcaccatcca gaactcttgt tccgttccca gggcgatatt
     9061 ttctttatcg ccataccaga tgcgttccgt accgaccttt ttcccacggt gcaaaatttg
     9121 caccgtagta aagttttgtt gcccccaacg cagtaatttt cttgcctctt cctcacgacc
     9181 ttttgcactg tcagccccca ttaccactgc aatgagacga cgctgcccat ctacagccga
     9241 agcaatgaga ttaaacccgg caccagaagt atgacccgtt ttcaggccgt caacattcat
     9301 ggttttatcc cacaataacc cgttacggtt ttgctgggtg ataccgttcc aggtgagact
     9361 tttctcactg tacatatgat aaaactcggg ctcgccgtgg atgatagcgc gagaaagcac
     9421 agctaaatca taagccgagc tatgctgacc tggtgcatcc agaccatgca ctgtttcaaa
     9481 atgcgtatcc ttgagatgca gcttctcggc atagttgttc atcatttcaa caaactgccg
     9541 ttgcccaccg gcaatatagt cagccagtgc aacacaagcg tcatttccgg aatccacaat
     9601 taaaccacgg cttaaatcac gtaccgatac gcgatcgccc tctttcaaaa acatcagtga
     9661 agaaccgaca aacactggat tatctttcgc ccacgcatcg cgccccacgg tgacaatatc
     9721 gtctggcgta atgcgatgac tatcgatagc gcgatccacg acatagcccg tcatcagctt
     9781 tgtcaggctg gcgggattgc gctgttgatg ctcattaccc gcggtgagga tctgaccggt
     9841 ggtgtaatcc atcaataccc aggagccggc atgaatctct ggaggctgag gtgaaaaagg
     9901 aatgttttcc gccgcaaaac cagacgataa gttaaaaatg aacaaagaag cagcaataat
     9961 aagacggcgt ttcaacagca aaccctcagg agtttcaaat agctgttctt tttacggaaa
    10021 tacttatgaa ctggctggaa taaagtgcaa gaaaatgtga ctaccctctc atttttatct
    10081 gacatgatct gttgccactc gctgtcaaat tgtcgcgcta aagctgatta gcacggtgat
    10141 atttgatact ctggcagaca gcagaaacaa cggatttaac ctaatgatga atgacggtaa
    10201 gcaacaatct acctttttgt ttcacgatta cgaaaccttt ggcacgcacc ccgcgttaga
    10261 tcgccctgca cagttcgcag ccattcgcac cgatgacgaa ttcaatgtca tcggcgaacc
    10321 cgaagtcttt tactgcaagc ccgcggatga ctatttaccc cagcctggag cagtattaat
    10381 taccggtatt accccgcagg aagctcgggc gaaaggagaa aacgaagccg catttgccgc
    10441 ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca acaatgtgcg
    10501 tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc cttacgcctg
    10561 gagctggcag catgataact cgcgctggga tttactggat gttatgcgtg cctgctatgc
    10621 cctgcgcccg gaaggaataa actggcctga aaatgatgac ggtctaccga gctttcgcct
    10681 tgagcattta accaaagcga atggtattga acatagcaac gcccacgatg cgatggctga
    10741 tgtgtacgcc actattgcga tggcgaaact ggtaaaaacg cgtcaaccac gcctgtttga
    10801 ttatctcttt acccatcgta ataaacacaa actgatggcg ttgattgatg ttccgcagat
    10861 gaaacccctg gttcacgttt ccggaatgtt tggggcatgg cgcggcaata ccagctgggt
    10921 ggcaccgctg gcgtggcatc ctgaaaatcg caatgccgta attatggtgg atttggcagg
    10981 agatatttcg ccattactgg aactggatag cgacacattg cgcgagcgtt tatataccgc
    11041 aaaaaccgat cttggcgata acgccgccgt tccggttaaa ctggtacata tcaataaatg
    11101 tccggtgctg gcccaggcga atacgctacg cccggaagat gccgaccgac tgggaattaa
    11161 tcgtcagcat tgcctcgata acctgaaaat tctgcgtgaa aatccgcaag tgcgtgaaaa
    11221 agtggtggcg atattcgcgg aagccgaacc gtttacgcct tcagataacg tggatgcaca
    11281 gctttataat ggctttttca gtgacgccga tcgtgcagca atgaaaattg tgcttgaaac
    11341 cgagccgcgt aatttaccgg cactggatat cacttttgtt gataaacgga ttgaaaagct
    11401 gttgttcaat tatcgggcac gcaacttccc ggggacgctg gattatgccg agcagcaacg
    11461 ctggctggag caccgccgcc aggtcttcac gccagagttt ttacaaggct atgcagaaga
    11521 gattcagatg ctggcgcagc agtatgccga cgataaagaa aaagtggcgc tgttaaaagc
    11581 gctttggcag tacgcggaag agattgtcta atgtgaagcc tccgccgctg gtacggcgga
    11641 ggacaatgtt gagtcaggct ttttgaacgg tgatgctcca cgctgcatcg ccaatctgct
    11701 gataatcggt gatggcatgc ccttcttctg ccgcccactg tgggattgct tcagttgcct
    11761 gggtgcaatc aaactcaatt accaactcat cgccgctggc catttctgcc agtgctgctt
    11821 ttgcttctat gagtggaaac ggacagacct gcgtcaccac atccagcttt ttaatcgcca
    11881 tctttacacc ttacttaatt tgccgcagca gttgccagtc gcgccttacg ctgcggtcga
    11941 acatacacaa gccaggacgc agtccagact ccgagaatca taaatacaag gccaacccag
    12001 ccctgccagg tcatcattgc agtcataacc agtccattac cgatagagca accacctgca
    12061 atactggcac cgaagcccat cagtacacca cctagcccgc tacgtactgt tgtttgtgca
    12121 tcagctgcgc gaacgcggaa ctcacggctc gctttggctg caataaatgc ccccacgaag
    12181 atccctaaca ccaggaaaac gccccagtta atgtatttca cgtcacccgc gaccagaaat
    12241 tgcagaatat tggccgttgg ggaagtgatg cctaacccaa acatacgtcc ggttgcttca
    12301 ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta cagctgtgac aaagggatgc
    12361 cagcgttttt caaacagaat atgagcgatc ccggttcggc gcggcggtaa ggtcgcgact
    12421 ttgagttttg gcttcttcaa ctctttcatc accacccaga gcgttatcac cagcaaaacg
    12481 gcaaccaacg gccacacaga caaattaaaa gtctcagcaa tagagttatg ttcagtagtg
    12541 tagtgctgca aggtttgatt taaaccactg gcgtgtggag aacgcatcac cgcactcatc
    12601 accatataag tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc gcgataccag
    12661 gtcccggtgg cacatccacc cgccagaaca attcccagcc cgaagagata cccacctata
    12721 acagtaccaa gccacgggaa cgccccggct tcgtaagtca gtaggcccgc ctgaattaac
    12781 gcaaaaaccc ccacgctttg taccgaaatt gcaatcagca aggcgtaaaa catgcgatta
    12841 tttttcgcga tatacatatc gcgaaaacca ccagtcaggc aaaaacgccc acgctgcatg
    12901 acaaatccca gcagagcacc acaaattagc ccgcttaata tcattgaaaa cataaattaa
    12961 ttaaccagat gaatgttaat ggggaaatta ttcatgactg gtggaatgca gaccaataac
    13021 caaattctct aaattagaac aaatggttat taatgaggat ttcgataaaa aagaataaaa
    13081 aaaccggagc caaatgttca ttcgactccg gtttattatt agaaatgatg attatgctac
    13141 gtcttcgtac tgcggaaccg ggttacggaa gctcttggtg acgcaagcca ggtaaatcag
    13201 accgatagca gcccagatca gacccagaac cattgagctt tcttccaggt taacccacag
    13261 cgcaccaacg gtcagcgcac cacacatcgg caggaacaga tactggaagt gatctttcag
    13321 cgtcttatta cgcttctcac ggatccagaa ctgcgagatg accgacaggt taacgaaggt
    13381 gaacgccacc aacgcaccaa agttgatcag cgccgtagcc attaccaggt cgaagttgat
    13441 tgccagcagg gcaatcgcgc caaccaggat gatgttcatc gccggagtac gccatttcgg
    13501 gtgtacataa ccgaagaaac ttttcgggaa cacgccgtca cgacccatta cgtacatcag
    13561 acgcgctacg cctgcgtgcg ccgccatacc ggacgccagt acggtaatgg tggagaagat
    13621 cagcgcaccg acctggaacg ctttacccgc aacgtacagc atgatttcag gctgtgacgc
    13681 atccggatct ttaaagcgag agatatccgg gaagtacagc tgcaggaagt aagttgcaaa
    13741 gatgaagatc atgccgccga tcagcgcggt caggaaaatc gcacgcggga tcacgcgctc
    13801 tgcatctttg gtttcttccg acagattgct gatgccgtca aagccggtaa aggagaagca
    13861 caggattgtc gccccggtaa tcatcgggat aacatgcgca tcgccagacc agaatggacg
    13921 agtgctcgcc agcgtaccag cgccttcacc ttcaaatacg ccataaacaa ccatgcccag
    13981 aatcaccgcg atcagcacta cctgcaacac gacgattaca gtgttgaagt tcgctacgga
    14041 tttcagacta cgcaggttaa aggcggtcat aaaggccacc agcgccacca caaacatcca
    14101 cgatgggatg gaaggcacca gagcttcaaa atagattttc gccagcagaa tgttgatcat
    14161 cggcgcgaac agatagtcga gcagagaaga ccaacccacc ataaagccga cagtcgggct
    14221 aatggatttc tgggcgtaag tatatgcaga gccagcagaa ggatagcggc gaaccagctt
    14281 cccgtagctc agagccgtaa acaggatcgc aatcaatgcg aacgcatagg ctgtcggcac
    14341 atgaccatcc gtgaggcctg aaacgatacc aaaagtatca aacagcgtca tcggctgcat
    14401 ataggcaaga cccatcatta caaccggaac taacgtaagc gttttacgca attccacgcg
    14461 agaggtgttt ggagtaacgt tatgcgacat ggtcattctc ctttacggtg ataaccgtcg
    14521 cgtaagcaaa aaattgcccc atttttttgg attcctcagc gacaacaact gtcgattttt
    14581 agtaaatatc tatccggtac gaagcccggc ctcttggtat gaatgattgg tttgaagcaa
    14641 aaaataaccg acgctgatga aacgtcggtt tttagtcatt ttttgacagc ggcgcattgt
    14701 gcctaaatgg gggggaaatg acaagagaat gagaggcttg tcataataat ttttctttaa
    14761 atggctgatt ttccgtcatc agattttgca taaacaccgg cgatcgcact atttgctaaa
    14821 atttcatccc gccaccagcc gctggcaagt cctgcggtct gttcattcca gccaatccag
    14881 acgggttcaa agagagtttg ggcgaccact ttcttctcga ttaacgcgcc actatcgaga
    14941 aaacgttgcg ccagataacg gggtaaatag ccgcagccga ggccgctaat ttgcagctcc
    15001 agcttggttt taaaatcgaa aacggtaatg gcttcttgtt cgtcgagaag ttgtgaggcc
    15061 gtagaagcgc ctgcctgcgc agtgtccccc accacaatcg cccgatatcg tttaatgata
    15121 cgacggttta atggttcttc ttcctgggct aacggatgat gaggcgcgac cgcaaagact
    15181 tgttccagat cgcccaaccg cgagaaacca aactcactgg aagatggtgg ctcatgcatc
    15241 gcgccaacaa taatatccgc tcgcccctga gtcagggcat cccatgaacc accgagtacg
    15301 ccattgataa acttcaggcg cgtcacgtta tgatgttgat aaaaagcttc aatgagtggc
    15361 gcaagcagag aaaaaggaaa cgtgtcgtcc acgccaatca ccagctcgtt ttcccagcct
    15421 tcatgcagtt tgatcgcctg cttttccagt tctcgcacgg tatgcagaac ttcccgcccc
    15481 ttctctaata acatttttcc ggtacgggtg aatttagcgc ggtgaccgct acgatcaagc
    15541 aattgaatat taaggtcgct ttccagcttg tgaacggtgt aactcagagc ggacggcgtc
    15601 ttgtagagtt tagccgacgc cgccgcaaaa ctgccttctt tttctaaggc atcgaggatc
    15661 atcaacacat ccagcagtgg tttcatacac gcccccttgc agtgcatgcg gcgtcctgct
    15721 ggcgtgatcg tttactccag cggcattacc agcggatcgg gatactggta ttcaaatccc
    15781 agttcattac aaatccgact gccatcaata atcttacctt tgccgctgtc cagactattt
    15841 ctgaattgcg gaggttccag ccccagtaaa cgggccatct gcggatagaa aacattacgc
    15901 gcagggtgag ctggcgcaca tatattatag atgtgtccgc ctttaggtgc ctgtaacaac
    15961 agagtgatag cgccaataac atcttctaaa tggactaaat taacaccatg ttcaccatca
    16021 ggcgcggttt ttccggcaaa gaagcgtccg ggatgacgcc ccggtccgac caggcccgca
    16081 agacgcagaa tatcgaccga agtaccgggt aaattgtgca gccagtcttc gagttcttct
    16141 aatactcgtc cactgttggt caccggatta cgcggggtgg tttctttcac cgtgccttgc
    16201 gcgtcgccat agacagacgt tgaactggta aaaataattc ggggaatacg atgggccagc
    16261 gcgctatcca ctaactcttg taccgcttgt aaatagaact catcgccggg gccgctacga
    16321 cgggccggaa gcgtaatgac cagcgcatcg gcatccatca gggcatccag atcgtcagaa
    16381 tcgcaaacta actcaggctc catgcgaagc agatagctat caatgccact cattcgggcc
    16441 gcttcgacac catcttgtgt ggttttactc ccggtgactt gccagcctcg cgctgaaagt
    16501 gacatcgcca gcggcatgcc taaccaccct aacccgacaa ttgcgacctt tttcatccgt
    16561 tatctcctga tacaccttag atctataagg ctacgctagc gtatcaaaac tgacaattca
    16621 ttctatgaat gaaatcgaaa ctgttagcaa atataccatt gcctgatgcg ctccgcttat
    16681 tagtcctaca aaacatcaat atattgaatt taggcaacat ttgtagacca gataaggcat
    16741 tcacgccgca tccggcagga acaaagccca ctttagttac agaacacaat gttcttgagg
    16801 gttttaatga catattttca cgattacttt tttaacctag tcactcttag ccccctcctt
    16861 tccccgctca ttcattaaac aaatccatcg ccataaaata tataaaaaag cccttgcttt
    16921 ctaacgtgaa agtggtttag gttaaaagac atcagttgaa taaacattca cagagacttt
    16981 tatgacacgc gttcaattta aacagcacca tcatcaccat catcctgact agtctttcag
    17041 gcgatgtgtg ctggaagaca ttcagatctt ccagtggtgc atgaacgcat gagaaagccc
    17101 ccggaagatc accttccggg ggctttttta ttgcgcggtt gataacggtt cagacaggtt
    17161 taaagaggaa taagaaaatg acagacaaca ctcgtttacg catagctatg cagaaatccg
    17221 gccgtttaag tgatgactct cgcgaattgc tggcgcgctg tggcattaaa atcaatcttc
    17281 acacccagcg cctgatcgcg atggcagaaa acatgccgat tgatattctg cgcgtgcgtg
    17341 acgacgacat tcccggtctg gtaatggacg gcgtggtaga tcttgggatt atcggcgaaa
    17401 acgtgctgga agaagagctg cttaaccgcc gcgcccaggg tgaagatcca cgctacttta
    17461 ccctgcgtcg tctggatttc ggcggctgcc gtctttcgct ggcaacgccg gttgatgatg
    17521 cctgggacgg cccgctctcc ttaaacggta aacgtatcgc cacctcttat cctcacctgc
    17581 tcaagcgtta tctcgaccag aaaggcatct cttttaaatc ctgcttactg aacggttctg
    17641 ttgaagtcgc cccgcgtgcc ggactggcgg atgcgatttg cgatctggtt tccaccggtg
    17701 ccacgctgga agcaaacggc ctgcgcgaag tcgaagttat ctatcgctcg aaagcctgcc
    17761 tgatacaacg cgatggcgaa atggaagaat ccaaacagca actgatcgac aagctgctga
    17821 cccgtattca gggtgtgatc caggcgcgcg aatcaaaata catcatgatg cacgcaccga
    17881 cccaacgtct ggatgaagtc atcgccctgc tgccaggtgc cgaacgccca accattctgc
    17941 cgctggcggg tgatcaacag cgcgtagcga tgcacatggt cagtagcgaa accctgttct
    18001 gggaaacgat ggaaaaactg aaagcgctgg gtgccagttc aatcctggtc ctgccgattg
    18061 agaagatgat ggagtgatcg ccatgagctt taacacaatc attgactgga atagctgtac
    18121 tgcaaagcaa caacgccagc tgttaatgcg cccggcgatc tccgcttctg aaagcattac
    18181 ccgcactgtt aacgatattc tcgatagcgt gaaagcacgc ggtgatgacg ccctgcggga
    18241 atatagcgcg aagtttgata aaaccacggt taccgcactg aaggtgtctg ctgaggaaat
    18301 tgccgccgcc agcgaacgcc tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa
    18361 gaatattgaa accttccaca ctgcgcaaaa actgccgccg gtagatgtag aaacgcagcc
    18421 aggcgtgcgt tgccagcaag tcacgcgccc ggtagcttca gttgggttgt atattcctgg
    18481 cggctccgcc ccgctcttct caacggtatt aatgctggca actccggcgc gtattgcggg
    18541 ctgtaaaaaa gtggtgttgt gctcaccgcc gccgattgcc gatgagatcc tttatgcggc
    18601 gcagctgtgc ggtgtgcagg acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct
    18661 ggcgtttggt acggaatctg tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt
    18721 tgtcaccgag gcaaaacgtc aggtgagcca gcgtctggac ggtgcggcga tcgatatgcc
    18781 cgcaggcccg tcggaagtgt tggtcattgc cgacagcggc gcaacgccgg atttcgtggc
    18841 ttctgatttg ctttctcagg ctgaacacgg cccggactca caggtgattt tactgacgcc
    18901 tgacgctgat atggcgcatc aagttgccga agccgtcgaa cgccagttag cagaactgcc
    18961 gcgtgccgaa accgcccgcc aggcactgaa cgccagccgc ctgatcgtga ctaaagattt
    19021 agcgcagtgc gtggagatct ccaaccagta cggcccggag cacctgatca ttcagacccg
    19081 caacgcccgc gaactggtcg atggcatcac cagcgccggt tcggtatttc ttggtgactg
    19141 gtcaccggaa tcggcaggtg attacgcctc cggaaccaac catgttctac cgacttacgg
    19201 ctacaccgcc acctgttcca gcctcggact ggcggatttc cagaagcgga tgaccgtgca
    19261 ggaactgtcg aaagtaggtt tctccgcgct ggcttcgacc attgaaacac tggccgccgc
    19321 cgagcgcctg accgcccaca aaaatgccgt tactttgcgt gttaacgccc ttaaggagca
    19381 agcatgagca ccgtgactat taccgattta gcgcgtgaaa acgtccgcaa cctgacgccg
    19441 tatcagtcgg cgcgtcgtct gggcggtaat ggcgacgtct ggctgaacgc caacgaatat
    19501 cccacagccg tggagtttca gcttactcag caaacgctca accgctaccc ggaatgccag
    19561 ccgaaagcgg tgattgaaaa ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg
    19621 gtcagccgtg gcgcggacga aggtattgaa ctactgattc gcgctttttg cgaaccgggt
    19681 aaagacgcca tcctctactg cccgccaacg tacggcatgt acagcgtcag cgctgaaacc
    19741 attggcgtcg agtgccgcac agtgccgacg ctggaaaact ggcaactgga cttacagggc
    19801 atttccgaca agctggacgg cgtaaaagtg gtctatgttt gcagccccaa caacccaacc
    19861 gggcaactga tcaatccgca ggattttcgc accctgctgg agttaacgcg cggtaaggcg
    19921 attgtggttg ccgatgaagc ctatatcgag ttttgcccgc aggcatcgct ggctggctgg
    19981 ctggcggaat atccgcacct ggctattttg cgcacactgt cgaaagcttt tgctctggcg
    20041 ggccttcgtt gcggatttac gctggcaaac gaagaagtca tcaacctgct gatgaaagtg
    20101 attgccccct acccgctctc gacgccggtt gccgacattg cggcccaggc gttaagcccg
    20161 cagggaatta tcgccatgcg cgaacgggtg gcgcaaatta ttgcagaacg cgaatacctg
    20221 attgccgcac tgaaagagat cccctgcgtg gagcaggttt ttgactctga aaccaactac
    20281 attctggcgc gctttaaagc ctccagtgca gtgtttaaat ctttgtggga tcagggcatt
    20341 atcttacgtg atcagaataa acaaccctct ttaagcggct gcctgcgaat taccgtcgga
    20401 acccgtgaag aaagccagcg cgtcattgac gccttacgtg cggagcaagt ttgatgagtc
    20461 agaagtatct ttttatcgat cgcgatggaa ccctgattag cgaaccgccg agtgattttc
    20521 aggtggatcg ttttgacaaa ctcgcctttg aaccgggcgt gatcccgcaa ctgctgaagc
    20581 tgcaaaaagc gggctacaaa ctggtgatga tcactaatca ggatggtctg ggaacacaaa
    20641 gtttcccgca ggcggatttc gatggcccgc acaacctgat gatgcagata ttcacctcgc
    20701 aaggcgtaca gtttgatgaa gtgctgattt gtccgcacct gcccgccgat gagtgcgact
    20761 gccgtaaacc gaaagtaaaa ctggtggagc gttatctcgc tgagcaagcg atggatcgcg
    20821 ccaacagtta tgtgattggc gatcgcgcga ccgatattca actcgctgaa aacatgggca
    20881 ttaatggttt acgctacgac cgcgaaaccc tgaactggcc aatgattggc gagcaactca
    20941 ccagacgtga ccgttacgct cacgtagtgc gtaataccaa agagacgcag attgacgttc
    21001 aggtgtggct ggatcgtgaa ggtggcagca agattaatac cggcgttggc ttctttgatc
    21061 acatgctgga tcagatcgcc acccacggcg gtttccgtat ggaaatcaac gtcaaaggcg
    21121 acctctatat cgacgatcac cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt
    21181 taaaaattgc cctcggcgat aaacgcggta tttgccgctt tggttttgtg ctgccgatgg
    21241 acgaatgcct tgcccgctgc gcgctggata tctctggtcg cccgcacctg gaatataaag
    21301 ccgagtttac ctaccaacgc gtgggcgatc tcagcaccga gatgatcgag cacttcttcc
    21361 gttcgctctc ttacaccatg ggcgtgacgc tccacctgaa aaccaaaggt aaaaacgatc
    21421 atcaccgtgt agagagcctg ttcaaagcct ttggtcggac cctgcgccag gccatccgcg
    21481 tggaaggcga taccctgccc tcgtcgaaag gagtgctgta atgaacgtgg tgatccttgg
    21541 tactggctgc gccaacctga actcggtgaa gtctgccatt gcgcgtcacg gttatgaacc
    21601 caaagtcagc cgtgacccgg acgtggtgtt gctggccgat aaactatttt tacccggcgt
    21661 tggcactgca caagcagcga tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa
    21721 agcctgtacc caaccggtgc tgggcatctg cttagggatg caactgctgg ggcggcgcag
    21781 tgaagagagc aacggcgtcg atttgctggg catcatcgac gaagacgtgc cgaaaatgac
    21841 cgactttggt ctgccgctgc cgcatatggg ctggaatcgc gtttacccgc aggcaggcaa
    21901 ccgcctgttt caggggattg aagacggcgc gtacttttac tttgttcaca gctacgcaat
    21961 gccagtcaat ccgtggacca tcgcccagtg taattacggc gaaccattca ccgcggcggt
    22021 acaaaaagat aacttctacg gcgtgcagtt ccatccggag cgttctggtg ccgctggcgc
    22081 taagttgctg aaaaacttcc tggagatgtg atgattattc cggcattaga tttaatcgac
    22141 ggcactgtgg tgcgtctcca tcagggcgat tacggcaaac aacgcgatta cggtaacgac
    22201 ccgctgccgc gcttacagga ttacgccgca caaggtgccg aagtgctgca tttggtggat
    22261 ctgaccgggg caaaagatcc ggcaaaacgt caaatcccgc tgattaaaac cctggtcgct
    22321 ggcgttaacg ttccggtgca ggttggcggc ggcgtgcgta gcgaagaaga cgtggcggcg
    22381 ttactggaag ctggggttgc gcgcgtggtg gttggctcca ctgcggtgaa atcaccagaa
    22441 atggtgaaag gctggtttga acgtttcggt gccgatgcct tagtgctggc gctggatgtc
    22501 cgtattgacg agcaaggcaa caagcaggtg gcggtcagcg gctggcaaga gaattcgggc
    22561 gtgtcactgg aacaactggt ggaaacctat ctgcccgtcg gcctgaaaca tgtgctgtgt
    22621 accgatatct cgcgcgacgg cacgctggca ggctctaacg tctctttata tgaagaagtg
    22681 tgcgccagat atccgcaggt ggcatttcag tcatccggcg gcattggcga cattaacgat
    22741 gttgctgccc tgcgtggcac tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa
    22801 ggtaaattca ccgtgaagga ggccatctca tgctggcaaa acgcataatt ccatgtctcg
    22861 acgttcgtga tggtcaggtg gtaaaaggcg tacagtttcg caaccatgaa atcattggcg
    22921 atatcgtgcc gctggcaaaa cgctacgccg aagaaggtgc agacgaactg gtgttctacg
    22981 atatcaccgc ttccagcgat ggccgtgtgg tagataaaag ttgggtatct cgcgtggcgg
    23041 aagtgatcga cattccgttc tgcgtggcgg gtgggattaa gtctctggaa gatgccgcga
    23101 aaattctttc ctttggcgcg gataaaattt ccatcaactc ccctgcgctg gcggacccga
    23161 cgttaattac tcgcctggcg gatcgatttg gcgtgcagtg tattgtggtg ggtattgata
    23221 cctggtacga cgctgaaacc ggtaaatatc atgtgaatca atataccggc gatgaaagcc
    23281 gcacccgcgt cactcagtgg gaaacgctcg actgggtaca ggaagtgcaa aaacgcggtg
    23341 ccggagaaat cgtcctcaac atgatgaatc aggacggcgt gcgtaacggt tacgacctcg
    23401 aacaactgaa aaaagtgcgt gaagtttgcc acgtcccgct gattgcttcc ggtggcgcgg
    23461 gcaccatgga acacttcctc gaagccttcc gcgatgccga cgttgacggc gcgctggcag
    23521 cttccgtatt ccacaaacaa ataatcaata ttggtgaatt aaaagcgtac ctggcaacac
    23581 agggcgtgga gatcaggata tgttaacaga acaacaacgt cgcgaactgg actgggaaaa
    23641 aaccgacgga ctgatgccgg tgattgtgca acacgcggta tccggcgaag tgttaatgct
    23701 gggctatatg aacccggaag ccttagacaa aaccatcgaa agcggcaaag tcaccttctt
    23761 ctcgcgcact aaacagcgac tgtggaccaa aggcgaaacg tcgggcaatt tcctcaacgt
    23821 agtaaatatt gcgccggact gcgacaacga cacgctactg gtactggcga atcctatcgg
    23881 accaacctgc cacaaaggca ccagcagctg cttcggcgac accgctcacc agtggctgtt
    23941 cctgtatcaa ctggaacaat tgctcgccga acgcaaatct gccgacccgg aaacctccta
    24001 caccgccaaa ctgtatgcca gcggcaccaa acgcattgcg cagaaagtgg gcgaagaagg
    24061 cgtggaaacc gcactggccg cgactgtaca tgaccgcttt gagctgacca acgaggcgtc
    24121 tgacttgatg tatcacctgc tggtattgtt gcaggatcag gatctggatt taacaacggt
    24181 gattgagaac ctgcgtaaac gtcatcagtg agttgcgggg taagcggatg cgatattgtt
    24241 gtcgcatccg gcaaaaaaaa cgggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
    24301 gaaaagatta cttcgcgttg taattacgca gagcgttacg ccccagcaca atccccgcgc
    24361 caaccatgcc gcccagcagc accgccagaa tcaaggtgat tgcctttttc ggactatcgc
    24421 gacgaatagg taacgtcggt ttcattacat agcggtaagc atgaatatca agatcatcaa
    24481 cctttaagtt ttcaatatcc aacaggtttt gacgtgtctg atagtagttt ggtgagaaca
    24541 ccaacggacg ggtcgcctca tgcttaatca tcgactccag cgcttcgctc cccaaaagga
    24601 acaacgtatc ttgcgtcaca tcttcagtct gctgaacctg tggctttgcc acctgcgcct
    24661 gattcgcata ctgcaacgct tcctgaatct gacggatacg cagctctttc tgctcctgcg
    24721 caactacttc ctgcgttctt aaagagtcct gcaagttttt ccgtcccaga gcaatgttgt
    24781 ctttgagatc cttttctagc tcttgattca ctttatcatc aacttgctga atgtattggg
    24841 ccaacttcat ttgtgctccc tctgcagttt gcccaacata agaaacagtc aatggtaatt
    24901 gctggttctt aacagaaggt tcgatggtaa gtttttctgg ttcttcctga ttatccagcg
    24961 tttctgctaa tgctgagaag gcagaactga agcgaccaat caacgtctcc tgcaaatccg
    25021 atactttcgg tgcagcctga ccatagataa cattcatggc attgttatag ccagcaattt
    25081 gccccacgtc gggctgagtg ataattgctg ttgacgtcca tttctccttc gctactgcca
    25141 aataaccaat agccagagca atagccacaa tgacggaaat gatgattgtc atcttgccac
    25201 gccacaactg cactagtaaa tcaatcaaat caatctgttc cgggtcatgg ttttgcccag
    25261 aaacattatt attttctact ctcatcatta ccctaactga cggaaatttc caagtccgca
    25321 tagtttagcg ataatccctg tgaatgctat aggataagtg ataaaaataa tgaattattg
    25381 cggaggtaac aaaagacaat cagggcgtaa atagccctga taacaagatg ttagtcgctg
    25441 ccaaagagat cgcgggtgta gaccttatct gccacatccc taagctcttc tgccatacgg
    25501 ttggaaataa tgacgtcggc ttgttgtttg aaggtggcga gatcacgttc caggcgagag
    25561 ttgaagaatg agtcttcttt catcactggc tcgtagatga ttacttcaac acctttcgcc
    25621 ttgatacgct tcataatccc ctgaatagaa gacgcacgga agttatctga accgctcttc
    25681 ataatcagac gataaatacc taccacttgc ggcttgcgtg acaaaatggc atcggcaata
    25741 aaatctttac gcgtgcggtt agcatcgaca attgccgaga tcaggttatt cggcacagac
    25801 tggtagttcg ccagtaactg cttggtatct ttcggcaaac aataaccacc ataaccaaac
    25861 gacggattgt tgtaatggtt gccaatacgt gggtcgagac aaacgccttc gattatttga
    25921 cgggaattca gacctaaact ttctgcgtag ctatccagtt cgttaaagta cgccacgcgc
    25981 atcgcgaggt aggtgtttgc aaaaagttta atcgcttctg cttcagtgga gtcggtaaac
    26041 agggttggga tattttgctt aatcgcgcct tcctgtaata acgcagcaaa acgttctgcg
    26101 cgttccgaac gctcaccgat tacaatacgt gacggatgaa ggttatcgta aagagcttta
    26161 ccttcgcgga gaaattccgg agagaaaata atattttcag tacgatattt cttatgcatc
    26221 gcttcggtaa aaccaacggg aaccgttgat ttgatgacca taaccgcata aggatttatc
    26281 tcaactacgt ctttaattac tgattctaca ctggatgtat tgaaataatt agttttagga
    26341 tcatagtcgg ttggagtggc gatgatgaca taatcagcat cccggtaggc ttcattttta
    26401 tctaatgtgg cattaaagtg tattttatct gattgcaaaa actgctgaat ttccttatca
    26461 acaataggag atatccgatc attcagcata gcaacgcgtg acggtaaaat atctaatgcc
    26521 acaacctcat gattttgagc gattagaagc ccgtttgaca agcctacata gccagtaccg
    26581 gaaatggtga ttttcatttc atgctctcag aattaactta actgtgaatc atgatgtttt
    26641 taacatcctg ataagagcta aaagttttaa cgctacgttg ttcgatggtc aacgcagcga
    26701 aaaacaattc agattttctt tataagaata gctgatatt
//
LOCUS       DAZKIF010000062        26367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZKIF010000062 DAZKIF010000000
VERSION     DAZKIF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.062"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            137..646
                     /locus_tag="ACHOTW_002544"
     CDS             137..646
                     /locus_tag="ACHOTW_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP9844628.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            643..2061
                     /gene="phrB"
                     /locus_tag="ACHOTW_002545"
     CDS             643..2061
                     /gene="phrB"
                     /locus_tag="ACHOTW_002545"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP9844629.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHOTW_002546"
     CDS             complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHOTW_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP9844630.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHOTW_002547"
     CDS             3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHOTW_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP9844631.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHOTW_002548"
     CDS             4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHOTW_002548"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP9844632.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHOTW_002549"
     CDS             5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHOTW_002549"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP9844633.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHOTW_002550"
     CDS             6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHOTW_002550"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP9844634.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7071..7862
                     /gene="nei"
                     /locus_tag="ACHOTW_002551"
     CDS             7071..7862
                     /gene="nei"
                     /locus_tag="ACHOTW_002551"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP9844635.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7859..8905)
                     /locus_tag="ACHOTW_002552"
     CDS             complement(7859..8905)
                     /locus_tag="ACHOTW_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9844636.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQPLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9054..10115)
                     /locus_tag="ACHOTW_002553"
     CDS             complement(9054..10115)
                     /locus_tag="ACHOTW_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9844637.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10112..10843)
                     /locus_tag="ACHOTW_002554"
     CDS             complement(10112..10843)
                     /locus_tag="ACHOTW_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9844638.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDHE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10858..13317)
                     /locus_tag="ACHOTW_002555"
                     /pseudo
     CDS             complement(10858..13317)
                     /locus_tag="ACHOTW_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(13367..13933)
                     /locus_tag="ACHOTW_002556"
     CDS             complement(13367..13933)
                     /locus_tag="ACHOTW_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9844639.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            complement(14320..15603)
                     /gene="gltA"
                     /locus_tag="ACHOTW_002557"
     CDS             complement(14320..15603)
                     /gene="gltA"
                     /locus_tag="ACHOTW_002557"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP9844640.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(15809..15916)
                     /locus_tag="ACHOTW_002558"
     CDS             complement(15809..15916)
                     /locus_tag="ACHOTW_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844641.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16297..16701
                     /gene="sdhC"
                     /locus_tag="ACHOTW_002559"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             16297..16701
                     /gene="sdhC"
                     /locus_tag="ACHOTW_002559"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9844642.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16695..17042
                     /gene="sdhD"
                     /locus_tag="ACHOTW_002560"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             16695..17042
                     /gene="sdhD"
                     /locus_tag="ACHOTW_002560"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP9844643.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            17042..18808
                     /gene="sdhA"
                     /locus_tag="ACHOTW_002561"
     CDS             17042..18808
                     /gene="sdhA"
                     /locus_tag="ACHOTW_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP9844644.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            18824..19540
                     /gene="sdhB"
                     /locus_tag="ACHOTW_002562"
     CDS             18824..19540
                     /gene="sdhB"
                     /locus_tag="ACHOTW_002562"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP9844645.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            19841..22642
                     /gene="sucA"
                     /locus_tag="ACHOTW_002563"
     CDS             19841..22642
                     /gene="sucA"
                     /locus_tag="ACHOTW_002563"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP9844646.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            22657..23874
                     /gene="odhB"
                     /locus_tag="ACHOTW_002564"
     CDS             22657..23874
                     /gene="odhB"
                     /locus_tag="ACHOTW_002564"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP9844647.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            23968..25134
                     /gene="sucC"
                     /locus_tag="ACHOTW_002565"
     CDS             23968..25134
                     /gene="sucC"
                     /locus_tag="ACHOTW_002565"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP9844648.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            25134..26003
                     /gene="sucD"
                     /locus_tag="ACHOTW_002566"
     CDS             25134..26003
                     /gene="sucD"
                     /locus_tag="ACHOTW_002566"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP9844649.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         6382 a         6590 c         7188 g         6207 t
ORIGIN      
        1 attcgctgct ttgatgatga tgtaaaatga ttgtttttga actggatata acagcttggt
       61 agggaagaaa gcataacccc acaaaattca tttaccatcg tctacactta ctcctgtaaa
      121 ccgttcagga gcagtaatga atcagcaacg atttgatgac agcactctaa tccgtatctt
      181 cgcgcttcat gagttacatc gactgaaaga acatggctta acgcgcgggg cgcttctcga
      241 ttatcacagc cgctataaac tcgtatttct ggctcattcc cagccggaat accgcaaact
      301 tggcccgttc gtggctgata ttcaccagtg gcaaaatctg gatgattttt acaaccagta
      361 ccgccaacgc gtaattgttt tgctttctca tcccgccaac ccgcgcgatc acaccaacgt
      421 tttgatgcac gttcagggtt attttcgccc gcatattgag tccacagaac gccagcagct
      481 ggctgcgctt atcgacagtt atcgccgtgg cgagcaaccg cttcttgcgc cgctgatgcg
      541 tatcaaacac tatatggcgc tttatcctta cgcctggctt tcagggcagc gttatttcga
      601 actttggccg cgtgtgatta acttgcgcca ttcaggagtt ttatgactac ccatctggtc
      661 tggtttcgcc aggatttacg tctgcatgat aatctcgcgc tggctgccgc ctgccgcaat
      721 tcatctgcac gcctgctggc gttgtatatc gctacaccac gccagtgggc ggcgcataat
      781 atgtcgccgc gtcaggctga acttatcaat gctcaactga acgggctaca aatcgcgctg
      841 gcggaaaaag gcattccttt attgttccgt gaagtggatg actttgccgc cagcgtcgaa
      901 atagttaaac aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat
      961 gaggtgaatg agcgggcgcg ggatgtgcag gttgagagaa ctctgcgtaa cgtggtgtgt
     1021 gaaggatttg atgacagcgt gatccttccg cctggcgcgg tgatgaccgg caatcatgag
     1081 atgtacaaag tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg
     1141 ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc
     1201 attacgctga attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa
     1261 gcggcgattg cgcaattacg ccagttttgc cagaacggtg ccggagaata tgagcaacaa
     1321 cgagattttc cggcagtgga aggcaccagt cgtttgtccg ccagcctggc aacgggcggg
     1381 ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt
     1441 ggggccggta gtgtctggct taatgagctg atctggcgcg agttctaccg tcatctgatg
     1501 acgtattacc cctcgttgtg taaacattgt ccgtttattg cctggacgga tcgtgtgcag
     1561 tggcagagca atcccgcaca tttacaggcc tggcaggaag gcaaaacggg atacccgatt
     1621 gtcgatgctg ccatgcgtca gcttaacagc actggctgga tgcataacag gctacggatg
     1681 attacagcca gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat
     1741 ttcatgtcgc agctgattga tggtgatttg gcagccaata acggtggctg gcagtgggcg
     1801 gcttctacgg gtactgatgc agcgccgtat tttcgtattt tcaacccgac aacccagggc
     1861 gagaaatttg accgcgaagg cgagtttatc cgccagtggc taccggaact gcgcaatgta
     1921 ccggggaaat cggtgcatga gccgtggaag tgggctgaga aagcaggtgt gaagctggat
     1981 tatccgcaac cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag
     2041 gcggcgcgga aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg
     2101 cgtgaacgcc ttatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt
     2161 aggcctgata agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttaagactcc
     2221 agcgccagcg cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccact
     2281 cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg
     2341 gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg
     2401 cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt taatacgccg
     2461 gtcacaccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc gataaacagt
     2521 tccgcaaagc ccatcaccgc caggcctaat accatcagtg gcagagaaga gtgaccatac
     2581 attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg
     2641 ccaagagcaa attttcccca gatgcgcacg gtacgattac ccgcgacact ctcttttacc
     2701 acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc
     2761 gcggtcggaa cggtataacc aaacatatcg cgattaacga agcggtcgat ataaaggcta
     2821 atcgagctac cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc
     2881 acaatcagcc tcagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc
     2941 agtacaccca ggccaatgat agtcgcgaca atcaaggcgt ataccgacca ttctttccag
     3001 aacagtacgg taatcagcgc aggcgttgcc accagcagaa ccagcagcca tccccagttc
     3061 ggcagcagaa agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgtgtgaaa
     3121 tgacgattgc cacataagaa aataaccaga cccgcgatca tacccaccgc cgccaggcca
     3181 aagcccatcg cccaactgta ctcttcctgg gcgtagccac aggcaatagg tgcgataata
     3241 gaccccacgt tacccgctgc atacatcagc gagaaaccgc catcacgacg cggatcggtt
     3301 ggctcataca gctcgccgag caggcaactg acattggatt taaacagacc atagccacaa
     3361 acgattatcg ccagggacag atagaggaat gacggatgga tctcacttgc acccaacacc
     3421 acatgaccga tcgccatcaa taacgccccc agcatcaccg ccatgcgatt gccgagaact
     3481 ttatccgcca aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta
     3541 aataactcgt aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc
     3601 agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa
     3661 atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca
     3721 cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga
     3781 ttatttatgc aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat
     3841 aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc
     3901 gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga
     3961 agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta
     4021 gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta
     4081 ccggtgtcac tgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg
     4141 tcattgtgca tcatggctac ttctggaaag gcgaatctcc ggtcattcgc ggcatgaagc
     4201 gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc
     4261 ttgacgcaca tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg
     4321 tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct gtgccgggac
     4381 tggagctggc ttcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata
     4441 ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg
     4501 atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca
     4561 ttcattcagc ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac
     4621 gtggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct
     4681 ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg
     4741 cgttgttatc tgataggtga aacggcggta gtgctggaac tggaaccgcc ggtgacgctg
     4801 gctagccaga aacggatctg gcgactggcg cagcgtctgg tggatatgcc gaatgtggtt
     4861 gaagccattc ccggcatgaa caatatcacg gtgattttgc gtaatcctga gtcgctggcg
     4921 ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gcgaggccct ggaacctgag
     4981 tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag gcggaccgga tttggcggtg
     5041 gtcgcggcgc attgcgggtt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa
     5101 tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa
     5161 caactacaca cgccacggcg cgctgaaccg cgcttgctcg ttccggcagg ttctgtcggg
     5221 atcggcgggc cgcagactgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt
     5281 ggtcatacct cactcagcct gtttgatccg gcgcgtgacg aacccatctt attacgtccg
     5341 ggagacagcg tgcgctttgt accgcaaaag gagggagtat gctgaagatt attcgtgcgg
     5401 gcatgtatac cactgtgcaa gatggcggtc gtcacggttt tcgccagtcg ggtatcagcc
     5461 actgcggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg ggtaatgatg
     5521 ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc gaaactgatg
     5581 ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat gccgtctgga
     5641 ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg
     5701 ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt tccgccggta atggggtcat
     5761 gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg aaggatggtg
     5821 accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc gttaaacagc
     5881 tgctgtgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg
     5941 cctcgcagga tgcattctgg cgttcgccct ggcagcttag ctcgcaaagt aaccgcatgg
     6001 gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg ttatctcacg
     6061 gtttgttacc gggcgtggtg caggtgccgc ataacgggca gcccattgtg ttgatgaacg
     6121 acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct gatatgtacc
     6181 atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg
     6241 cactgaaagc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg cggctgcaca
     6301 atgaaaattg acctgaacgc cgatctgggc gaaggctgca ccagcgacgc agagctatta
     6361 acgctggttt cctctgccaa tattgcctgt ggatttcatg caggcgatgc gcaaaccatg
     6421 caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca tccgagtttt
     6481 cccgacagga aaaattttgg tcgcagcgct atgcagctgc cgccagaaac cgtttacgcc
     6541 cagacgctgt atcaaattgg cgcgctggca acgattaccc gtgcgcaagg tggtgtaatg
     6601 cgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc acaactggca
     6661 gacgccatcg ccagagcggt atacgcttgc gatccagcat tggttctcgt tgggctggca
     6721 ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga caacgcgcga ggaagtgttt
     6781 gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc gaagccagtc aggcgcgctg
     6841 attgaagacg aagaacaggc gcttgcgcaa acgttggaga tggtgcaaca cggcagagtc
     6901 aaaagtatca ccggcgaatg ggcaacggtc acggcgcaaa cggtctgcct gcatggcgac
     6961 ggcgagcacg cactggcgtt cgcccgccga ctacgctctg catttgccga aaaggggatt
     7021 gttgtcgcag cataaccccg attaataaag aatgaaaaaa ggatatcacc atgcctgaag
     7081 gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
     7141 ctgatgtctg gtttgccttc ccgcagttaa aaacttatca atcacaactt atcggtcaac
     7201 acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa
     7261 cgctctacag ccataatcag ctttatggcg tctggcgcgt ggttgatacc ggcgaagagt
     7321 cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaacc attctgcttt
     7381 atagcgcctc ggatattgag atgttgaccc cggaacaact gaccacgcat ccgtttttac
     7441 aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg aaagaacggt
     7501 tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
     7561 tggctgggct tggcaattat ttgcgtgtgg agatcctctg gcaggttggg ttgactggaa
     7621 atcataaagc gaaagacctc aatgcggcgc aactggatgc actcgcacac gcgttactgg
     7681 atattcctcg gttttcttac gctacgcggg ggcaggtgga tgagaataag catcatgggg
     7741 cgctgtttcg ctttaaggtt tttcatcgtg atggcgaact gtgcgaacgc tgtggcggca
     7801 tcattgagaa aaccacgctg tcatctcgcc cgttttactg gtgtccgggc tgccagcact
     7861 aggccgtacg tttcggcgca taggttgaaa taaaccgcgc aatggcaggc cctgtcagca
     7921 aaatactgaa caggcgcagg ggttgcatcg ccataatgag cgccatatcg gcattgctcc
     7981 ctgcggcgat gaccgccacg gtatcaagcc cgccggggct ggtggcgagg taggcagtca
     8041 taaaatcaat gtgcataaac cgggtcagtc cccacgccat acccgcacaa atagccagca
     8101 gagcaaaaat cgacagcagg atttgtggta gcgggcgtag tgcccgcagt aagatttgtt
     8161 tatcgaaacc aaggccaatc cgccagccaa ttgccatata cgccatcgcc agtagccatt
     8221 ccggtagttc gatggtgatg agctgaccag actggagcac tgcgcccgcc agcatcggga
     8281 tgagcatcgt gccggaagga agacgcagca ggcatcccac tgtaccggca acgaccgcga
     8341 gcaaaatagt aagcaggaga ttaatgctca ctggcgggaa ccagacgata tgctggttaa
     8401 ccgcttcagc gttatcgccc agcatcattc gcgtgaccag aactgcggcc cccgcgacaa
     8461 acagcactcg cagatactgc ataaacgcca ccaggcggat atctgcgccg taatcctgcg
     8521 ccatcgcgac cattgccgcc gccccgccag gcgaggagcc ccaggcacca gtatttccgg
     8581 gcagtgagct atagcgcacc agtagccatc ccacgatagc gctggaaagc aacgtcacca
     8641 gtaaaatagc taacacaatc ggccagttaa cctccagcgt ggtcaggatc gaaccggtca
     8701 agttttgcgc aatcatgcag ccaagaatgg cctgcgcggc gagaaaagcg gagcggggga
     8761 gttgcagggt aaccccgcgc atactgaaga tgatcccggc gatcatcggt ccaagtaaga
     8821 gtgccgccgg gagatgtaat ccgaggaaac caatagaaag gagaagtgat agcacacata
     8881 acattcccca ctgcaaaact ggcatccttt gctccctcat tattttctct ttggtgtatc
     8941 gatacagcat aaagaatgcc gggcatggaa aaaataacgg taatcaataa aagccggaaa
     9001 attggcaatt aaccgaagaa gccctctccg gctggaaagg gcaataggct aaatcagttg
     9061 tagttaattt tgaaaagtac cactgtatca aagggaccaa cgtcgatggg cgtacccggc
     9121 gtggatgaca attctgccgt gtaggttttt tcatacacta acaggtcgct ggtaaaatcg
     9181 gcatactcac tgtatttgtt gaaagtatag ggtgaggcat tttcatccaa caatcgaagc
     9241 ttcaagccat tgccaatgag cagtgattta tcttcgtcga taagggtttc atcggtataa
     9301 aacgaagagc tgactttaaa accgtcggta cactggtcgt tttgtgattt ggttgtagtt
     9361 attttaaatt gctgcgacat cgtatggcga cgaatatcca gcacgttaaa tcggccaaaa
     9421 tcaacgacct gggtttcagg tgagatagca aagttgacgc tgcaatccag cacgcggata
     9481 ttttccagac cggtaatgtg atatttcaga ttcttcgcgg tagggtcttc gttcacgctg
     9541 cctgcgccat caaactgtac gacgatgtaa tcgctgagtt tgctctgata atcatggggc
     9601 ggcatggcgc gaactttaac gtaaagccgc atacgcgcca caaacgtccg cgatttgtgc
     9661 ataacggttg gatcgccgga gcaaagttta ttttgccagc ccatcgcaat gatctgctcc
     9721 ggggtgtaaa tatcaatatt cttactgtcg atgcactgat tcgtgtcgat gcggcttttc
     9781 ccaaggcttg cgtcatagtc cacgccttca taggttacgc ccaattgata atagggatcc
     9841 tgaataccgg ggtaagggtt tacccaggcg tagacgtgct cgctttcaaa attaccgttt
     9901 gtgttgttgt cacagtagac cggaattttt atatcgtcag attcccagat tttatcgccg
     9961 ggtttggaat tggccggaac ggcgaagggc ataatagctt ccgatttttc tacagcgccg
    10021 tttgcggtac caaaatagca gttcagcgcc agcgccgctt ttagcggcaa aaaaatcaga
    10081 cagatggcga gcaacacgcc tttggcgaat ctcatttttt attctccacc ggctgcaggc
    10141 ggcattgccg ctggcaggag agcgtgttca tacgcaatgc gccgtagtcg tccatgtagc
    10201 caagataaaa ctgcttgccg ctatagctgc ccgtgctggt ggtgaccgaa ctgaacggtg
    10261 cgaccatcgt gcttttaaag ccaggcagca cgcctttctc atcccgccca agataggcaa
    10321 tagtcagata gtatccggtc ggggttttca gggtaagctg gttattctgc tggctaacct
    10381 gtagctgttg ctcaacatga tcgcccattt ttttgcgtaa tgcggcgggt cgccagaata
    10441 gcttaatgcg actttggacg gcgacttgca ggatcgcatg gccctcgctg ccttccggga
    10501 caggcggaat ttcacgcagg ttatagtaaa agagagattc gtgatcggtg ggtaatttcg
    10561 cggtggcggc ttgtttcatg atccgaacct gcgaagtcgc ttttggctcc agtcgctgga
    10621 taggcggcag ggcgacgaga aattcatcgc ttttctctcc cttttcattt tctatccatg
    10681 aataggcgag gtaggggagt ttatcgctgc ggttttccac gcgcaggctg gttgctttat
    10741 ctttgctatt aaagatgatg cgcgtgcggt caggctgcac tgcgccgtgg ctgcacatac
    10801 tggcaaccag aagaatcaaa gggaatcctt taacaaatgt catcgtcttt tccttaatta
    10861 atgacagggt aaaatcaggc gcttagtcac atcttccaga tgttccggca gatgaatggc
    10921 acacgttttc tggtcgcccc agtgcacggt aaattgttga ttttcatcga ccccactcag
    10981 ccatgcgtga ccgttttcgc caaccatccc gacgctgaca ccgccttcag cctggcgaac
    11041 atcggcaccg agcggcggga aatgcccgtt agcatcgctg ataatgacgt tgagatcttt
    11101 cccggcgcgg gacgccagcg atttaaagcc gatggcgcct tcggtctagg tttctttcac
    11161 cacgttttcg gaaacggtaa cgccgtcagg caagtcgttc atattgaccg ccaccgtcgt
    11221 aggctggtag ctggaaacaa acggtaccac ggcaatacca aagcggttgg tgtagtcaat
    11281 attgccctga atcggaatat cgcccacgcc gtcggtgctg accatcaggc gtggttcatt
    11341 ggtggaactg cggcgatgga atgccgcgcc atgttgagtc gcggtaaacg agccgctcca
    11401 actggcactg gcggaagtgt aatcattggc ggcataggtt ccggtcagat cccattcccc
    11461 cgccgaactg aggtgttggt aattgccgct gacctgcgca ccgttatccg ggcgatcgga
    11521 ttgtattcca gccgacattc cccaactgtt tcgttcatcc agggtatcgt tccacgacat
    11581 gcggtgggtg gtagtattac tgttgttctg catgtcataa cccagtcgac cactttcccc
    11641 aatcggtaag gagatcgaaa agtaaatctg gttgtcgcga tctttatctt cataatgggt
    11701 ggtgttgaac gaggtcgaga cagaaatatc tttccagtcg ccaatgtcga cgttaaaacc
    11761 gacggtaatg ttggccgtgg tggaggtatc cgcattccac cagctctgat gcaggatgtt
    11821 ggcgtagaga ttaagattca gcagagtaat aggctgaccg aaggagaggc tgattgtttg
    11881 cttttcatcc tgcgcgtcgg cgtcattgta tttgtgatca atgtagttag cgtagctgtg
    11941 aaaatggcga tcggagaagc gataggctgc caatgagatg gtgctgttgg tcgcatcaac
    12001 ctgtttgctg tagttaaagc ggtagctgta accctgctca tcctgttggg tatcaaaatg
    12061 gctgtcggcc caggtcacgt caaacgacag cgcccccatc cagagcatat tttgcccaat
    12121 acccagcgcg ccggaacgat agtcatcgcc tgcgagaagc atgccgccat atagtgaagt
    12181 gttggagagc attccccagg agacttcatg gctaatgaag gtttcatcct cggtgtggtg
    12241 cgacatagag gaacgcgggc gaccggcagc cagtttatag cgcacctggc cctgacgcgt
    12301 caggaacggg gtagaggccg ctgaaacctg gaagttattc acccgtccat cttcttcgct
    12361 cactttgaca tccagtgtac cttgcactga ctgattcagg tcatcaataa taaatgggcc
    12421 tggtggaact tttttctggt aaatcacgcg accagaatgg cttatcgtga ccgtggcgtt
    12481 agtctgtgcg atgccgctga tttgcggcgc gtagccgcgt aactcccagg ggagcattcg
    12541 atcgtcgctg gctaacgccg caccggtata ggagaaacca tcaaaaatgt tagagctaaa
    12601 atcggtttcg cccagggtta atcgggaacc tatttgcggt aacgggcgga acaggtaggt
    12661 ccgtgaaatc gccccagatt gttcacggtt gtcgccgcta tcggactggc taagctgata
    12721 atcgctgcgc agacgccatg ccccggcgtt gaggcccgtg gtgccatagg cgttcaggtt
    12781 gttgctgctg ctgccgcttt gtgggcgata agtgctggca aacaggttgt aatccatcag
    12841 gaagccagga ataccgttat tccacgttga gggtggcgtc cagttttctg aatgccacgc
    12901 cagccatgcc tgcgggattg taatattgag ctgttgattt gcctgatcga aagtaaagat
    12961 gatttcaggg aagacactaa aatcgacaca ttcttttatt gccggtaatt ttttacgaaa
    13021 gtcagattta agtccgaaga gttcgatcag cgattcattg atacaaggaa taattttatc
    13081 gccagatttt tgccagcgaa tttgttgccc attactgatt ttattattat taaccgtaac
    13141 actaacaaag taatcgccgg gagctacgac gcctttttga ttgagcaacg aaatatcaac
    13201 gctatcccga atcgacttat cgagaacgtt aaggttaaat tcagctgtca gcgcaggagg
    13261 ggttaccatt gccagacagg aaagaacaga gagtcgataa atattcacgg catccatacc
    13321 tgaacaatat tcgcaaaagg cggctaatca gccgccttaa aaaggattat tggtaatcca
    13381 gaacgtaagt tgcgttagca gtaacggccc ccgcggtaac cgaaactgcg ttatccatct
    13441 gttccatcca ggcaaagaag tttaacgttt gttctgttga acttgccaat aagtcaattg
    13501 ttggcgcttc tgttcccaat gtaacaaatg attcatcttt gttcatgaga cgtacgccaa
    13561 cgccggtagc ctcgccagca ctgatgttac taaggagcgg ggttgcacct gtcgttttag
    13621 ccgtgctgtc gaaggtcacg cctacctttg agaccacgcc acccttgcca tcatcccagc
    13681 ctgcaatatc acagttgatt aagcgaatat tgacaggttt tttctcgcta tgatgttctc
    13741 gatttatgtg ggatgtggtt acttcaccca ggtcaatgtt catatcaagg tcatcagggt
    13801 gaatttcaca tggtgcttca ataacttcac ctttaaagtg gatttcacca gatccttcat
    13861 ctgcagcaaa aacaggtgaa gcgaatagta gagatatggc cagggcggcc agtgtttttt
    13921 gtcctttaaa catacatgaa tccttcatgg gctatgataa agtatactaa ttattccgac
    13981 agaatagtgg ttttttgttc atgtaataat attaatgttg ctacattatg tttgagagtt
    14041 tctcaaagct ctcgaatcat aaataaagtg aattaatcct attacgagtt tatttaattg
    14101 tgggtgctac ccaatgtatt ctacatcgat tggcgggata acggtggtgg tgaatgaaaa
    14161 attaggattt tatttagtta tcggatatgt attcttgtaa cgtgtaatta tgtatttgtg
    14221 tccgattacg tttttttgtc gatagacata actaacgaac aaaaaaaagc ccgccagata
    14281 ttcaggcggg ctaaacgata tatggttcaa atcagataat taatgtttaa catctgattt
    14341 aaagtctcgt ttttcatagc cggtgtacaa ctggcgcgga cgggcgatct tcatgccgtc
    14401 actgtgcatt tcgctccagt gagcgatcca gccaacggta cgggccatag cgaagatgac
    14461 ggtgaacatg gaagacggaa tacccatcgc tttcaggatg ataccagagt agaaatcgac
    14521 gttcgggtac agtttcttct cgataaagta cgggtcgttc agcgcgatgt tttccagctc
    14581 catagccact tccagcaggt catctttggt gcccagctct ttcagaactt catggcaggt
    14641 ttcacgcatt acggtggcgc gcgggtcgta atttttgtac acgcggtgac cgaagcccat
    14701 caggcggaaa gaatcatttt tatctttcgc acgacgaaca aattccggaa tgtgtttaac
    14761 ggagctaatt tcttccagca ttttcagcgc cgcttcgtta gcaccaccgt gtgcaggccc
    14821 ccacagtgaa gcaatacctg ctgcgataca ggcaaacggg ttcgcacccg aagagccagc
    14881 ggtacgcacg gtggaggtag aggcgttctg ttcatggtca gcgtgcagga tcagaatacg
    14941 gtccatagca cgttccagaa tcggattaac ttcatacggt tcgcacggcg tggagaacat
    15001 catattcagg aagttaccgg cataggagag atcgttgcgc ggataaacaa atggctgacc
    15061 aatggaatac ttgtaacaca tcgctgccat ggtcggcatt ttcgacagca ggcggaacgc
    15121 ggcaatttca cgatgacgag gattgttaac atccagcgag tcgtgataga acgccgccag
    15181 cgcgccggta ataccacaca tgactgccat tggatgtgag tcgcgacgga aagcgtggaa
    15241 cagacgggta atctgctcgt ggatcatggt atgacgggtc accgtagttt taaattcgtc
    15301 atactgttcc tgagtcggtt tttcaccatt cagcaggatg taacaaactt ccaggtagtt
    15361 agaatcggtc gccagctgat cgatcgggaa accgcggtgc agcaaaatac cttcatcacc
    15421 atcaataaaa gtaattttag attcgcagga tgcggttgaa gtgaagccag ggtcaaaggt
    15481 gaacacacct tttgaaccga gagtacggat atcaataaca tcttgaccca gcgtgccttt
    15541 cagcacatcc agttcaacag ctgtgtcccc gttgagggtg agttttgctt ttgtatcagc
    15601 catttaaggt ctccttagcg ccttattgcg taagactgcc ggaacttaaa tttgccttcg
    15661 cacatcaacc tggctttacc cgtttgttat ttggctcgcc gctctgtgaa agaggggaaa
    15721 acctgggtac agagctctgg gcgcttgcag gtaaaggatc cattgatgac gaataaatgg
    15781 cgaatcaagt acttagcaat ccgaattatt aaacttgtct accactaata actgtcccga
    15841 atgaattggt caatacttcc acactgttac ataagttaat cttaggtgaa ataccgactt
    15901 cataactttt acgcattata tgcttttcct ggtaatgttt gtaacaactt tgttgaatga
    15961 ttgtcaaatt agatgattaa aaattaaata aatgttgtta tcgtgacctg gatcactgtt
    16021 caggataaaa cccgacaaac tatatgtagg ttaattgtaa tgattttgtg aacggcctat
    16081 actgccgcca ggtctccgga acaccctgca atcccgagcc acccagcgtt gtaacgtgtc
    16141 gttttcgcat ctggaagcag tgttttgcat gacgcgcagt tatagaaagg acgctgtctg
    16201 acccgcaagc agaccggagg aaggaaatcc cgacgtctcc aggtaacaga aagttaacct
    16261 ctgtgcccgt agtccccagg gaataataag aacagcatgt gggcgttatt catgataaga
    16321 aatgtgaaaa aacaaagacc tgttaatctg gacctacaga ccatccggtt ccccatcacg
    16381 gcgatagcgt ccattctcca tcgcgtttcc ggtgtgatca cctttgttgc agtgggtatc
    16441 ctgctgtggc ttctgggtac cagcctctct tcccctgaag gtttcgagca agcttccgcg
    16501 attatgggca gcttcttcgt caaatttatc atgtggggca tccttaccgc tctggcgtat
    16561 cacgtcgtcg taggtattcg ccacatgatg atggattttg gctatctgga agaaacattc
    16621 gaagcgggta aacgctccgc caaaatctcc tttgttatta ctgtcgtgct ttcacttctc
    16681 gcaggagtcc tcgtatggta agcaacgcct ccgcattagg acgcaatggc gtacatgatt
    16741 tcatcctcgt tcgcgctacc gctatcgtcc tgacgctcta catcatttat atggtcggtt
    16801 ttttcgccac cagtggcgag ctgacatatg aagtctggat cggtttctgc gcctctgcgt
    16861 tcaccaaagt gttcaccctg ctggcgctgt tttctatctt gatccatgcc tggatcggca
    16921 tgtggcaggt gttgaccgac tacgttaaac cgctggcttt gcgcctgatg ctgcaactgg
    16981 tgattgtcgt tgcactggtg gtttacgtga tttatggatt cgttgtggtg tggggtgtgt
    17041 gatgaaattg ccagtcagag aatttgatgc agttgtgatt ggtgccggcg gcgcaggtat
    17101 gcgcgcggcg ctgcaaattt cccagagcgg ccagacctgt gcgctgctct ctaaagtctt
    17161 cccgacccgt tcccataccg tttctgcgca aggcggcatt accgttgcgc tgggtaatac
    17221 ccatgaagat aactgggaat ggcatatgta cgacaccgtg aaagggtcgg actatatcgg
    17281 tgaccaggac gcgattgaat atatgtgtaa aaccgggccg gaagcgattc tggaactcga
    17341 acacatgggc ctgccgttct cgcgtctcga tgatggtcgt atctatcaac gtccgtttgg
    17401 cggtcagtcg aaaaacttcg gcggcgagca ggcggcacgc actgcggcag cggctgaccg
    17461 taccggtcac gcactgttgc acacgcttta tcagcagaac ctgaaaaacc acaccaccat
    17521 tttctccgag tggtatgcgc tggatctggt gaaaaaccag gatggcgcgg tggtgggttg
    17581 taccgcactg tgcatcgaaa ccggtgaagt ggtttatttt aaagcccgcg ctaccgtgct
    17641 ggcgactggc ggagcagggc gtatttatca gtctaccact aacgcccaca ttaacaccgg
    17701 cgacggcgtc ggcatggcta tccgcgcagg cgtaccggtg caggatatgg aaatgtggca
    17761 attccacccg accggcattg ccggtgcggg cgtactggtc accgaaggtt gccgtggtga
    17821 aggcggttat ctgctgaaca aacatggcga acgctttatg gagcgttatg cgccgaacgc
    17881 caaagacctg gcgggccgtg atgtggtggc gcgttcaatc atgattgaaa tccgtgaagg
    17941 tcgcggctgt gatggtccgt gggggccaca cgcgaaactg aaactcgatc acctgggtaa
    18001 agaagttctc gaatcccgtc tgccgggtat cctggagctt tcccgtacct tcgctcacgt
    18061 tgatccggtg aaagagccga ttccggttat cccaacctgt cactacatga tgggcggtat
    18121 tccgaccaaa gttaccggtc aggcgctgac tgtgaatgag aaaggcgaag atgtggttgt
    18181 tccggggctg ttcgcggtag gtgaaattgc ctgcgtatcg gtacacggtg ctaaccgtct
    18241 gggcggcaac tcgctgctgg acctggtggt ctttggtcgc gcagcgggtc tgcatctgca
    18301 agagtctatc gccgagcagg gcgcattgcg cgatgccagc gagtctgatg ttgaagcatc
    18361 tttggatcgt ctgaaccgct ggaacaataa tcgtaacggt gaagatccgg tggcgattcg
    18421 taaagcgctg caagaatgta tgcagcataa cttctcggtc ttccgtgaag gtgatgcgat
    18481 ggctaaaggg cttgagcagt tgaaagtgat ccgcgagcgt ctgaaaaatg cccgtctgga
    18541 tgacacttcc agcgagttca acacccagcg cgttgagtgc ctggaactgg ataacctgat
    18601 ggaaacggcg tatgcaacgg ctgtttctgc caacttccgt accgaaagcc gtggcgcaca
    18661 tagccgcttc gacttcccgg atcgtgatga tgaaaactgg ctgtgccact ccctgtatct
    18721 gccagagtcg gaatccatga cgcgccgaag cgtcaacatg gaaccgaaac tgcgcccggc
    18781 attcccgccg aagattcgta cttactaatg cggagacagg aaaatgagac tcgagttttc
    18841 aatttatcgc tataacccgg atgttgatga tgctccgcgt atgcaggatt acaccctgga
    18901 agcggaagaa ggtcgcgaca tgatgctgct ggatgcgctt atccagctga aagagaaaga
    18961 tcccagcctg tcgttccgcc gctcctgccg tgaaggtgtg tgcggttccg acggtctgaa
    19021 catgaacggc aagaatggtc tggcctgtat taccccgatt tcggcactca accagccggg
    19081 caagaagatt gtgattcgcc cgctgccagg tttaccggtg atccgcgatt tggtggtaga
    19141 catgggacaa ttctatgcgc aatatgagaa aattaagcct tacctgttga ataatggaca
    19201 aaatccgcca gctcgcgagc atttacagat gccagagcag cgcgaaaaac tcgacgggct
    19261 gtatgaatgt attctctgcg catgttgttc aacctcttgt ccgtctttct ggtggaatcc
    19321 cgataagttt atcggcccgg caggcttgtt agcggcatat cgtttcctga tcgatagccg
    19381 tgataccgag actgacagcc gcctcgacgg tttgagcgat gcattcagtg tattccgctg
    19441 tcatagcatc atgaactgcg tcagtgtatg tccgaagggg ctgaacccga cgcgcgccat
    19501 cggccatatc aagtcgatgt tgttgcaacg taatgcgtaa accgtaggcc tgataagacg
    19561 cgcaagcgtc gcatcaggca accagtgccg gatgcggcgt gaacgcctta tccggcctac
    19621 aagtcattac ccgtaggcct gataagcgca gcgcatcagg cgtaacaaag aaatgcagga
    19681 aatctttaaa aactgcccct gacactaaga cagtttttaa aggttccttc gcgagccact
    19741 acgtagacaa gagctcgcaa gtgaaccccg gcacgcacat cactgtgcgt ggtagtatcc
    19801 acggcgaagt aagcataaaa aagatgctta agggatcacg atgcagaaca gcgctttgaa
    19861 agcctggttg gactcttctt acctctctgg cgcaaaccag agctggatag aacagctcta
    19921 tgaagacttc ttaaccgatc ctgactcggt tgacgctaac tggcgttcga cgttccagca
    19981 gttacctggt acgggagtca aaccggatca attccactct caaacgcgtg aatatttccg
    20041 ccgcctggcg aaagacgctt cacgttactc ttcaacgatc tccgaccctg acaccaatgt
    20101 gaagcaggta aaagtcctgc agctcattaa cgcataccgc ttccgtggtc accagcatgc
    20161 gaatctcgat ccgctgggac tgtggcagca agataaagtg gccgatctgg atccgtcttt
    20221 ccacgatctg accgaagcag acttccagga gaccttcaac gtcggttcat ttgccagcgg
    20281 caaagaaacc atgaaactcg gcgagctgct ggaagccctc aagcaaacct actgcggccc
    20341 gattggtgcc gagtatatgc acattaccag caccgaagaa aaacgctgga tccaacagcg
    20401 tatcgagtct ggtcgcgcga ctttcaatag cgaagagaaa aaacgcttcc taagcgaact
    20461 gaccgccgct gaaggccttg aacgttacct cggtgcaaaa ttccctggcg caaaacgctt
    20521 ctcgctggaa ggcggtgacg cgttaatccc gatgcttaaa gagatgattc gccacgctgg
    20581 caacagcggc acccgcgaag tggttctcgg gatggcgcac cgtggtcgtc tgaacgtgct
    20641 ggtgaacgtg ctgggtaaaa aaccgcaaga cttgttcgac gagtttgccg gtaaacataa
    20701 agaacacctc ggcacgggtg acgtgaaata ccacatgggc ttctcgtctg acttccagac
    20761 cgatggcggc ctggtacatc tggcgctggc gtttaacccg tctcaccttg agattgtcag
    20821 cccggtcgtt atcggttctg ttcgtgcccg tctggacaga cttgatgagc cgagcagcaa
    20881 caaagtgctg ccaatcacca ttcacggtga cgccgcagtg accgggcagg gcgtggttca
    20941 ggaaactctg aacatgtcga aagcgcgtgg ttatgaagtt ggcggtacgg tacgtatcgt
    21001 tatcaacaac caggttggct tcaccacctc taacccgctg gatgcccgtt caacgccgta
    21061 ctgtactgat atcggtaaga tggttcaggc accgattttt cacgttaacg cggatgatcc
    21121 ggaagccgtt gcctttgtga cccgtctggc gctcgatttc cgtaacacct ttaaacgtga
    21181 tgtcttcatc gacctggtgt gctaccgccg tcacggccac aacgaagccg acgagccgag
    21241 cgcaacccag ccgctgatgt atcagaaaat caaaaaacat ccgacgccgc gcaaaatcta
    21301 tgctgacaag ctggagcagg aaaaagtggc gacgctggaa gatgccaccg agatggttaa
    21361 cctgtaccgc gatgcgctgg atgctggcga ttgcgtagtg gcagagtggc gtccgatgaa
    21421 catgcactct ttcacctggt cgccgtacct caaccacgaa tgggacgaag agtacccgaa
    21481 taaagttgag atgaagcgcc tgcaggagct ggcgaaacgc atcagcacgg tgccggaagc
    21541 ggttgaaatg cagtctcgcg ttgccaaaat ttatggcgat cgccaggcga tggctgccgg
    21601 tgagaaactg ttcgactggg gcggcgcgga aaacctcgct tacgccacgc tggttgacga
    21661 aggcattccg gttcgcctgt cgggtgaaga ctccggtcgc ggtaccttct tccaccgcca
    21721 cgcggtgatc cacaaccagt ctaacggttc cacttacacg ccgctgcaac acatccataa
    21781 cggccagggc gcgttccgtg tctgggactc cgtactttct gaagaagcag tgctggcgtt
    21841 tgaatacggt tatgccaccg cagaaccacg cactctgact atctgggaag ctcagttcgg
    21901 tgacttcgcc aacggtgcgc aggtggttat cgaccagttc atctcctctg gcgaacagaa
    21961 atggggccgg atgtgtggtc tggtgatgtt gctgccgcac ggttacgaag ggcaggggcc
    22021 ggagcactcc tccgcgcgtc tggaacgtta tctgcaactt tgtgctgagc aaaacatgca
    22081 ggtgtgcgta ccgtctaccc cggcacaggt ttaccacatg ctgcgtcgtc aggcgctgcg
    22141 cgggatgcgt cgtccgctgg tcgtgatgtc gccgaaatcc ctgctgcgtc atccgctggc
    22201 ggtttccagc ctcgaagaac tggcgaacgg caccttcctg ccagccatcg gtgaaatcga
    22261 cgagcttgat ccgaagggcg tgaagcgcgt agtgatgtgt tctggtaagg tttattacga
    22321 cctgctggaa cagcgtcgta agaacaatca acacgatgtc gccattgtgc gtatcgagca
    22381 actctacccg ttcccgcata aagcgatgca ggaagtgttg cagcagtttg ctcacgtcaa
    22441 ggattttgtc tggtgccagg aagagccgct caaccagggc gcatggtact gcagccagca
    22501 tcatttccgt gaagtgattc cgtttggggc ttctctgcgt tatgcaggcc gcccggcctc
    22561 cgcctctccg gcggtagggt atatgtccgt tcaccagaaa cagcaacaag atctggttaa
    22621 tgacgcgctg aacgtcgaat aaataaagga tacacaatga gtagcgtaga tattctggtc
    22681 cctgacctgc ctgaatccgt agccgatgcc accgtcgcaa cctggcataa aaaacccggt
    22741 gacgcagtcg tacgtgatga agtgctggta gaaatcgaaa ctgacaaagt ggtactggaa
    22801 gtaccggcat cagcagacgg cattctggat gcggttctgg aagatgaagg tacaacggta
    22861 acgtctcgtc agatccttgg tcgcctgcgt gaaggcaaca gcgccggtaa agaaaccagc
    22921 gccaaatctg aagagaaagc gtccactccg gcgcaacgcc agcaggcgtc tctggaagag
    22981 caaaacaacg atgcgttaag cccggcgatc cgtcgcctgc tggctgaaca taacctcgac
    23041 gccagcgcca ttaaaggcac cggtgtgggt ggtcgtctga cccgtgaaga tgtggaaaaa
    23101 catctggcga aagctccggc gaaagagtct gcaccggcag cggctgctcc ggcggcgcaa
    23161 ccggctctgg ctgcacgtag cgaaaaacgt gtcccgatga ctcgcctgcg taagcgtgtg
    23221 gcagagcgtc tgctggaagc gaaaaactcc accgccatgc tgaccacgtt caacgaagtc
    23281 aacatgaagc cgattatgga tctgcgtaag cagtacggtg aagcgtttga aaaacgccac
    23341 ggcatccgtc tgggctttat gtccttctac gtgaaagcgg tggttgaagc cctgaaacgt
    23401 tacccggaag tgaatgcttc tatcgacggc gatgacgtgg tttaccacaa ctatttcgac
    23461 gtcagcatgg cggtttctac gccgcgcggc ctggtgacgc cggtactgcg tgatgtcgat
    23521 accctcggca tggcagacat cgagaagaaa atcaaagagc tggcagttaa aggccgtgac
    23581 ggcaagctga cggttgaaga tctgaccggt ggtaacttca ccatcaccaa cggtggtgtg
    23641 ttcggttccc tgatgtctac gccgatcatc aacccgccgc agagcgcaat tctgggtatg
    23701 cacgctatca aagatcgtcc gatggctgtg aatggtcagg ttgagatcct gccgatgatg
    23761 tacctggcgc tgtcctacga tcaccgtctg atcgatggtc gcgaatccgt gggcttcctg
    23821 gtaacgatca aagagttgct ggaagatccg acgcgtctgc tgctggacgt gtagtagttt
    23881 aagtttcacc tgcactgtag accgacgtgg atagggtgtc ggtctacggt ttaaaagata
    23941 acgattactg aaggatggac agaacacatg aacttacatg aatatcaggc aaaacaactg
    24001 tttgcccgct atggcttacc agcaccggtg ggttatgcct gtactactcc gcgcgaagca
    24061 gaagaagccg cttcaaaaat cggtgccggt ccgtgggtag tgaaatgtca ggttcacgct
    24121 ggtggccgcg gtaaagcggg cggtgtgaaa gttgtaaaca gcaaagaaga catccgtgct
    24181 tttgcagaaa actggctggg caagcgtctg gtaacgtatc aaacagatgc caatggccaa
    24241 ccggttaacc agattctggt tgaagcagcg accgatatcg ctaaagagct gtatctcggt
    24301 gccgttgtag accgtagttc acgtcgcgtg gtctttatgg cctccaccga aggcggcgtg
    24361 gaaatcgaaa aagtggcgga agaaactccg cacctgatcc acaaagttgc gcttgatccg
    24421 ctgactggcc cgatgccgta tcagggacgc gagctggcgt tcaaactggg tctggaaggt
    24481 aaactggttc agcagttcac caaaatcttc atgggcctgg cgaccatttt cctggagcgc
    24541 gacctggcgc tgatcgaaat caacccgctg gtcatcacca aacagggcga tctgatttgc
    24601 ctcgacggca aactgggcgc tgatggcaac gccctgttcc gccagcctga tctgcgcgaa
    24661 atgcgtgacc agtcgcagga agatccgcgt gaagcacagg ctgcacagtg ggaactgaac
    24721 tacgttgcgc tggacggtaa catcggttgt atggttaacg gcgcaggcct ggcgatgggt
    24781 acgatggaca tcgttaaact gcacggcggc gaaccggcta acttcctgga cgttggcggc
    24841 ggcgcaacca aagaacgtgt aaccgaagcg ttcaaaatca tcctctctga cgacaaagtg
    24901 aaagccgttc tggttaacat cttcggcggt atcgttcgtt gcgacctgat cgctgacggt
    24961 atcatcggtg cggtagcgga agtgggtgtt aacgtaccgg tcgtggtacg tctggaaggt
    25021 aacaacgccg aactcggcgc gaagaaactg gctgacagcg gcctgaatat tattgcagca
    25081 aaaggtctga cggatgcagc tcagcaggtt gttgccgcag tggaggggaa ataatgtcca
    25141 ttttaattga taaaaacacc aaggttatct gccagggctt taccggtagc caggggactt
    25201 tccactcaga acaggccatt gcatacggca ctaaaatggt tggcggcgta accccaggta
    25261 aaggcggtac tacccacctc ggcctgccgg tgttcaacac cgtgcgtgaa gctgttgctg
    25321 ctactggcgc taccgcttct gttatctacg taccagcacc gttctgcaaa gactccattc
    25381 tggaagccat cgacgcaggc atcaaactga ttatcaccat cactgaaggc atcccgacgc
    25441 tggatatgct gaccgtgaaa gtgaagctgg atgaagcagg cgttcgtatg atcggcccga
    25501 actgcccagg cgttatcact ccgggtgaat gcaaaatcgg tattcagcct ggtcacattc
    25561 acaaaccggg taaagtgggt atcgtttccc gttccggtac actgacctat gaagcggtta
    25621 aacagaccac ggattacggt ttcggtcagt cgacctgtgt cggtatcggc ggtgacccga
    25681 tccctggctc taactttatc gacattctcg aaatgttcga aaaagatccg cagaccgaag
    25741 cgatcgtgat gatcggtgag atcggcggta gcgctgaaga agaagcagct gcgtacatca
    25801 aagagcacgt taccaagcca gttgtgggtt acatcgctgg tgtgactgcg ccgaaaggca
    25861 aacgtatggg ccacgcgggt gccatcattg ccggtgggaa agggactgcg gatgagaaat
    25921 tcgctgctct ggaagccgca ggcgtgaaaa ccgttcgcag cctggcggat atcggtgaag
    25981 cactgaaaac tgttctgaaa taaatatctg taataagaaa tagccctcgc cgcttccctc
    26041 tacaggaatg gcgaagggct gtcggtttcg acatggttgg ccatcgtatg atggcctttt
    26101 ttatgcctta aacggttaac cacggtcgaa atctgactgc aggcttgact gcatgaccac
    26161 agccactgtg gattgccgca accgaccgca agccacgaat aagaacgacg gcgaatttcg
    26221 aatcgttacc tgatgcactg gcttcccagc gtttgaccgt agcgttaagt aaatcaacaa
    26281 aagcgatgtt caccgctttt cgtcaagtcg tgccatttgt cagccaggat cgggtcttta
    26341 ccccggatat tgcagcatcg caaaaat
//
LOCUS       DAZKIF010000063        26212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZKIF010000063 DAZKIF010000000
VERSION     DAZKIF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.063"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..159
                     /gene="tuf"
                     /locus_tag="ACHOTW_002567"
     CDS             <1..159
                     /gene="tuf"
                     /locus_tag="ACHOTW_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9844650.1"
                     /translation="ELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGA
                     GVVAKVLG"
     gene            342..536
                     /gene="bfd"
                     /locus_tag="ACHOTW_002568"
     CDS             342..536
                     /gene="bfd"
                     /locus_tag="ACHOTW_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP9844651.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            609..1085
                     /gene="bfr"
                     /locus_tag="ACHOTW_002569"
     CDS             609..1085
                     /gene="bfr"
                     /locus_tag="ACHOTW_002569"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP9844652.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(1082..1549)
                     /locus_tag="ACHOTW_002570"
     CDS             complement(1082..1549)
                     /locus_tag="ACHOTW_002570"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9844653.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1928..2239
                     /gene="rpsJ"
                     /locus_tag="ACHOTW_002571"
     CDS             1928..2239
                     /gene="rpsJ"
                     /locus_tag="ACHOTW_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9844654.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2272..2901
                     /gene="rplC"
                     /locus_tag="ACHOTW_002572"
     CDS             2272..2901
                     /gene="rplC"
                     /locus_tag="ACHOTW_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9844655.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2912..3517
                     /gene="rplD"
                     /locus_tag="ACHOTW_002573"
     CDS             2912..3517
                     /gene="rplD"
                     /locus_tag="ACHOTW_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9844656.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3514..3816
                     /gene="rplW"
                     /locus_tag="ACHOTW_002574"
     CDS             3514..3816
                     /gene="rplW"
                     /locus_tag="ACHOTW_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9844657.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3834..4655
                     /gene="rplB"
                     /locus_tag="ACHOTW_002575"
     CDS             3834..4655
                     /gene="rplB"
                     /locus_tag="ACHOTW_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9844658.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4672..4950
                     /gene="rpsS"
                     /locus_tag="ACHOTW_002576"
     CDS             4672..4950
                     /gene="rpsS"
                     /locus_tag="ACHOTW_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9844659.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4965..5297
                     /gene="rplV"
                     /locus_tag="ACHOTW_002577"
     CDS             4965..5297
                     /gene="rplV"
                     /locus_tag="ACHOTW_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9844660.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5315..6016
                     /gene="rpsC"
                     /locus_tag="ACHOTW_002578"
     CDS             5315..6016
                     /gene="rpsC"
                     /locus_tag="ACHOTW_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9844661.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6029..6439
                     /gene="rplP"
                     /locus_tag="ACHOTW_002579"
     CDS             6029..6439
                     /gene="rplP"
                     /locus_tag="ACHOTW_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9844662.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6439..6630
                     /gene="rpmC"
                     /locus_tag="ACHOTW_002580"
     CDS             6439..6630
                     /gene="rpmC"
                     /locus_tag="ACHOTW_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9844663.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6630..6884
                     /gene="rpsQ"
                     /locus_tag="ACHOTW_002581"
     CDS             6630..6884
                     /gene="rpsQ"
                     /locus_tag="ACHOTW_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9844664.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7049..7420
                     /gene="rplN"
                     /locus_tag="ACHOTW_002582"
     CDS             7049..7420
                     /gene="rplN"
                     /locus_tag="ACHOTW_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9844665.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7431..7745
                     /gene="rplX"
                     /locus_tag="ACHOTW_002583"
     CDS             7431..7745
                     /gene="rplX"
                     /locus_tag="ACHOTW_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9844666.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7760..8299
                     /gene="rplE"
                     /locus_tag="ACHOTW_002584"
     CDS             7760..8299
                     /gene="rplE"
                     /locus_tag="ACHOTW_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9844667.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8314..8619
                     /gene="rpsN"
                     /locus_tag="ACHOTW_002585"
     CDS             8314..8619
                     /gene="rpsN"
                     /locus_tag="ACHOTW_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9844668.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8653..9045
                     /gene="rpsH"
                     /locus_tag="ACHOTW_002586"
     CDS             8653..9045
                     /gene="rpsH"
                     /locus_tag="ACHOTW_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9844669.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9058..9591
                     /gene="rplF"
                     /locus_tag="ACHOTW_002587"
     CDS             9058..9591
                     /gene="rplF"
                     /locus_tag="ACHOTW_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9844670.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9601..9954
                     /gene="rplR"
                     /locus_tag="ACHOTW_002588"
     CDS             9601..9954
                     /gene="rplR"
                     /locus_tag="ACHOTW_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9844671.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9969..10472
                     /gene="rpsE"
                     /locus_tag="ACHOTW_002589"
     CDS             9969..10472
                     /gene="rpsE"
                     /locus_tag="ACHOTW_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9844672.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10476..10655
                     /gene="rpmD"
                     /locus_tag="ACHOTW_002590"
     CDS             10476..10655
                     /gene="rpmD"
                     /locus_tag="ACHOTW_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9844673.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10659..11093
                     /gene="rplO"
                     /locus_tag="ACHOTW_002591"
     CDS             10659..11093
                     /gene="rplO"
                     /locus_tag="ACHOTW_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9844674.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11101..12432
                     /gene="secY"
                     /locus_tag="ACHOTW_002592"
     CDS             11101..12432
                     /gene="secY"
                     /locus_tag="ACHOTW_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9844675.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12464..12580
                     /gene="rpmJ"
                     /locus_tag="ACHOTW_002593"
     CDS             12464..12580
                     /gene="rpmJ"
                     /locus_tag="ACHOTW_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9844676.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12727..13083
                     /gene="rpsM"
                     /locus_tag="ACHOTW_002594"
     CDS             12727..13083
                     /gene="rpsM"
                     /locus_tag="ACHOTW_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9844677.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13100..13489
                     /gene="rpsK"
                     /locus_tag="ACHOTW_002595"
     CDS             13100..13489
                     /gene="rpsK"
                     /locus_tag="ACHOTW_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9844678.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13523..14143
                     /gene="rpsD"
                     /locus_tag="ACHOTW_002596"
     CDS             13523..14143
                     /gene="rpsD"
                     /locus_tag="ACHOTW_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9844679.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14169..15158
                     /gene="rpoA"
                     /locus_tag="ACHOTW_002597"
     CDS             14169..15158
                     /gene="rpoA"
                     /locus_tag="ACHOTW_002597"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9844680.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15199..15582
                     /gene="rplQ"
                     /locus_tag="ACHOTW_002598"
     CDS             15199..15582
                     /gene="rplQ"
                     /locus_tag="ACHOTW_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9844681.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15689..16057
                     /gene="yhdN"
                     /locus_tag="ACHOTW_002599"
     CDS             15689..16057
                     /gene="yhdN"
                     /locus_tag="ACHOTW_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP9844682.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16068..16493
                     /gene="zntR"
                     /locus_tag="ACHOTW_002600"
     CDS             16068..16493
                     /gene="zntR"
                     /locus_tag="ACHOTW_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP9844683.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16549..16767
                     /gene="arfA"
                     /locus_tag="ACHOTW_002601"
     CDS             16549..16767
                     /gene="arfA"
                     /locus_tag="ACHOTW_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP9844684.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16764..17174)
                     /gene="mscL"
                     /locus_tag="ACHOTW_002602"
     CDS             complement(16764..17174)
                     /gene="mscL"
                     /locus_tag="ACHOTW_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9844685.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17304..18680)
                     /gene="trkA"
                     /locus_tag="ACHOTW_002603"
     CDS             complement(17304..18680)
                     /gene="trkA"
                     /locus_tag="ACHOTW_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP9844686.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18702..19991)
                     /gene="rsmB"
                     /locus_tag="ACHOTW_002604"
     CDS             complement(18702..19991)
                     /gene="rsmB"
                     /locus_tag="ACHOTW_002604"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9844687.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20037..20984)
                     /gene="fmt"
                     /locus_tag="ACHOTW_002605"
     CDS             complement(20037..20984)
                     /gene="fmt"
                     /locus_tag="ACHOTW_002605"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9844688.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20999..21508)
                     /gene="def"
                     /locus_tag="ACHOTW_002606"
     CDS             complement(20999..21508)
                     /gene="def"
                     /locus_tag="ACHOTW_002606"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9844689.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21638..22762
                     /gene="dprA"
                     /locus_tag="ACHOTW_002607"
     CDS             21638..22762
                     /gene="dprA"
                     /locus_tag="ACHOTW_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFP9844690.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22734..23207
                     /gene="smg"
                     /locus_tag="ACHOTW_002608"
     CDS             22734..23207
                     /gene="smg"
                     /locus_tag="ACHOTW_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP9844691.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23236..23778
                     /gene="yrdD"
                     /locus_tag="ACHOTW_002609"
     CDS             23236..23778
                     /gene="yrdD"
                     /locus_tag="ACHOTW_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP9844692.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23783..24355
                     /gene="tsaC"
                     /locus_tag="ACHOTW_002610"
     CDS             23783..24355
                     /gene="tsaC"
                     /locus_tag="ACHOTW_002610"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP9844693.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24360..25178
                     /gene="aroE"
                     /locus_tag="ACHOTW_002611"
     CDS             24360..25178
                     /gene="aroE"
                     /locus_tag="ACHOTW_002611"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9844694.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25175..25432
                     /gene="yrdB"
                     /locus_tag="ACHOTW_002612"
     CDS             25175..25432
                     /gene="yrdB"
                     /locus_tag="ACHOTW_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP9844695.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25408..25962)
                     /gene="yrdA"
                     /locus_tag="ACHOTW_002613"
     CDS             complement(25408..25962)
                     /gene="yrdA"
                     /locus_tag="ACHOTW_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9844696.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6719 a         6215 c         6810 g         6468 t
ORIGIN      
        1 gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca acatcaaaat ggttgttacc
       61 ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg caatccgtga aggcggccgt
      121 accgttggcg cgggcgttgt agctaaagtt ctgggctaat tacacgttaa ttagttttga
      181 attgaaaagg gcgcttcggc gccctttttg ctgcccgtaa tttgagctcg aaatatttag
      241 tcgtaatttt gctcgctgaa ctatggcgca aataaaactg tgctattgta atcataacta
      301 ttctcattta cactttgcgc agaatttgaa cgggagcaat catgtacgtt tgtttgtgta
      361 atggtgtaag tgacaaaaaa attcgccagg cagtccgtca gtttcatccg caatctttcc
      421 aacaattgcg taagtttatt cctgtgggaa atcaatgtgg taagtgcatt cgcgccgcgc
      481 gcgaagtgat gcaggacgag ttgatgcaga tgccagaatt taaagagatt gcctgaagcc
      541 cacgcttatt ttttgacatc ccgctagccc catctacgct ctaatagtgg aagcggaggg
      601 actataaaat gaaaggtgat gttaaaatca taaattatct caataaacta ttgggaaatg
      661 agcttgtcgc aatcaatcag tattttctcc atgcccggat gtttaaaaac tggggactga
      721 tgcgtcttaa tgacgtcgaa taccatgaat ccatcgatga gatgaaacac gctgataaat
      781 atatcgagcg tattttgttt ttagaaggaa tccccaactt acaggattta ggtaagctag
      841 ggatcggtga agatgtcgaa gagatgctgc aatccgacct gcgattagag ctggaaggtg
      901 cgaaggatct gcgtgaggct atcgcgtatg ccgacagcgt tcatgactat gttagccgcg
      961 atatgatgat cgagatcctt gccgatgaag aaggtcatat cgactggctc gaaactgaac
     1021 tggatctcat cggtaaaatc ggcctacaaa attacctgca atcacaaatt aaagttaatg
     1081 attaacggcc tatcagcaaa ctatcccagc ctataacgaa acccgcagcc gccagaaatg
     1141 gcccaaaagg cagcgggttt tttaatgatt cttttcctct catgagcaga ccaataacta
     1201 cggctccgca agcgaatgag gcagcaagga aaaccagtcg tggtaagaag gcccatgaat
     1261 gccaggcgcc aagggcggcg agaaacttca catcaccata gcctaatcct tctttatggc
     1321 gcagtatgcg atacccccag taaatgacag cgaatgtgcc gtaaccaata attgcccccc
     1381 ataacgcatc ggctaaacca tcaggatgac aaacttgata gaacaaaaga cctgaccaga
     1441 gtaacgggca cgtaaatctg tcaggtaaca agccatgctt tgcatcccag aaaaaaagta
     1501 gggctgataa acaagcgtac aaaattagaa agggaagggt agtgtccata aaacatcctc
     1561 tgtaattgga gtatcaccat cgaataactc cggatgctca gcaacagaga gagcgctgat
     1621 ttgttaacct gattccggtg aaaaacgcta tcttcacaac cttacaaatt tcgtggaatg
     1681 cagctcgcaa tctgcgcgtt gcgattaatt tctgcccgat gcttgcgtca ggctcagatt
     1741 tgcgtataat gcgcgggctt gtcgtagttg acagcaggtt caatctgaac ctcagttagc
     1801 cgaatttggc tacctaacaa tgctcccaat cggggagcta cgtaagaacg gttacactct
     1861 cccatcaatc gtaatgggtc tgaggagtaa tcattttcgt ttataaaata attggagctc
     1921 tggtctcatg cagaaccaaa gaatccgtat ccgcctgaaa gcgtttgatc atcgtctgat
     1981 cgatcaagca accgcggaaa tcgtcgagac tgccaagcgc actggtgcgc aggtccgtgg
     2041 tccgatcccg ctgccgacac gcaaagagcg cttcactgtt ctgatctccc cgcacgtcaa
     2101 caaagacgcg cgcgatcagt acgaaatccg tactcacttg cgtctggttg acatcgttga
     2161 gccaaccgag aaaaccgttg atgctctgat gcgtctggat ctggctgccg gtgtagacgt
     2221 gcagatcagc ctgggttaat caggtcattg agcgattgag aggttgaaac aatgattggt
     2281 ttagtcggta aaaaagtggg tatgacccgt atcttcacag aagacggcgt ttctatccca
     2341 gtaaccgtaa tcgaagttga agcaaaccgc gttactcagg ttaaagacct ggctaacgat
     2401 ggctaccgtg ctattcaggt gaccaccggt gctaaaaaag ctaaccgtgt gaccaagcct
     2461 gaagctggcc acttcgctaa agctggcgta gaagctggcc gtggtctgtg ggaattccgc
     2521 ctggctgaag gcgaagagtt cactgtaggt cagagcatta gcgttgaact gtttgctgac
     2581 gttaaaaaag ttgacgtaac tggcacctct aaaggtaaag gtttcgcagg taccgttaag
     2641 cgctggaact tccgtaccca agacgctact cacggtaact ccttgtctca ccgcgttccg
     2701 ggttctatcg gtcagaacca gactccgggc aaagtgttca aaggcaagaa aatggcaggt
     2761 cagatgggta acgaacgtgt aaccgttcag agccttgacg tagtacgcgt tgacgctgag
     2821 cgcaacctgc tgctggttaa aggtgctgtc ccgggtgcaa ccggtagcga cctgatcgtt
     2881 aaaccagctg tgaaggcgta aggagatagc aatggaatta gtattgaaag acgcgcagag
     2941 cgcgctgact gtttccgaaa ctaccttcgg tcgtgatttc aacgaagcgc tggttcacca
     3001 ggttgttgtt gcttatgcag ctggtgctcg tcagggtact cgtgctcaga agactcgtgc
     3061 tgaagtaact ggttccggta aaaaaccgtg gcgccagaaa ggcaccggcc gtgcgcgttc
     3121 tggttctatc aagagcccga tctggcgttc tggtggcgtg acctttgctg ctcgtccgca
     3181 ggaccacagt caaaaagtta acaagaagat gtaccgcggc gcgctgaaaa gcatcctgtc
     3241 cgaactggta cgtcaggatc gtctgatcgt tgtcgagaag ttctctgtag aagcgccgaa
     3301 aactaagctg ctggcacaga aactgaaaga catggctctg gaagatgtgc tgatcatcac
     3361 cggtgagctg gacgaaaacc tgttcctggc tgcgcgcaac ctgcacaagg ttgacgtacg
     3421 cgatgcaact ggtatcgacc cggttagcct gatcgccttc gacaaagtcg taatgactgc
     3481 tgatgctgtt aagcaagttg aggagatgct ggcatgattc gtgaagaacg tctgctgaag
     3541 gtgctgcgtg caccgcacgt ttctgaaaaa gcgtctactg cgatggaaaa atccaacacc
     3601 atcgtactca aagttgctaa agacgcgacc aaagcagaaa tcaaagctgc tgtgcagaaa
     3661 ctgtttgaag tcgaagtcga agtcgttaac accctggtag ttaaagggaa agttaaacgt
     3721 cacggacagc gtatcggtcg tcgtagcgac tggaaaaaag cttacgtcac cctgaaagaa
     3781 ggccagaatc tggacttcgt tggcggcgct gagtaagtcg gaggagtaat acaatggcag
     3841 ttgttaaatg taaaccgaca tctccgggtc gtcgccacgt agttaaagtg gttaaccctg
     3901 agctgcacaa gggcaaacct tttgctccgt tgctggaaaa aaacagcaaa tccggtggtc
     3961 gtaacaacaa tggccgtatc accactcgtc atatcggtgg tggccacaag caggcttacc
     4021 gtattgttga cttcaaacgc aacaaagacg gtatcccggc agttgttgaa cgtcttgagt
     4081 acgatccgaa ccgttccgcg aacatcgcgc tggttctgta caaagacggt gaacgccgtt
     4141 acatcctggc ccctaaaggc ctgaaagctg gcgaccagat tcagtctggc gttgatgctg
     4201 caatcaaacc aggcaacacc ctgccgatgc gcaacatccc ggttggttct actgttcata
     4261 acgtagaaat gaaaccaggt aaaggcggtc agctggcacg ttccgctggt acttacgttc
     4321 agatcgttgc tcgcgacggt gcttatgtca ccctgcgtct gcgttctggt gaaatgcgta
     4381 aagtcgaagc agactgccgt gcaactctgg gcgaagttgg caatgctgag catatgctgc
     4441 gcgttctggg taaagcaggt gctgcacgct ggcgtggtgt tcgtccgacc gttcgcggta
     4501 ccgcgatgaa cccggtagac cacccacatg gtggtggtga aggtcgtaac tttggtaagc
     4561 acccggtaac tccgtggggc gttcagacca aaggtaagaa gacccgcagc aacaagcgta
     4621 ctgataaatt catcgtacgt cgccgtagca aataatttta gaggataagc catgccacgt
     4681 tctctcaaga aaggtccttt tattgacctg cacttgctga agaaggtaga gaaagcggtg
     4741 gaaagcggag acaagaagcc cctgcgcact tggtcccgtc gttcaacgat ctttcctaac
     4801 atgatcggtt tgaccatcgc tgtccataat ggtcgtcagc acgttccggt atttgtaacc
     4861 gacgaaatgg tcggtcacaa actgggtgaa ttcgcaccga ctcgtactta tcgcggccac
     4921 gctgctgata aaaaagcgaa gaagaaataa ggtaggagga agagatggaa actatcgcta
     4981 aacatcgcca tgctcgttct tctgctcaga aggttcgcct tgttgctgac ctgattcgcg
     5041 gtaagaaagt gtcgcaggct ctggatattt tgacctacac caacaagaaa gcggctgtac
     5101 tggtcaagaa agttctggaa tctgccattg ctaacgctga acacaacgat ggcgctgaca
     5161 ttgacgatct gaaagttacg aaaattttcg tagacgaagg cccgagcatg aagcgcatta
     5221 tgccgcgtgc aaaaggtcgt gcagatcgca tcctgaagcg caccagccac atcactgtgg
     5281 ttgtgtccga tcgctgagac tctggagact agcaatgggt cagaaagtac atcctaatgg
     5341 tattcgcctg ggtattgtaa aaccatggaa ctctacctgg tttgcgaaca ccaaagaatt
     5401 cgctgacaac ctggacagcg attttaaagt acgtcagtac ctgactaagg aactggctaa
     5461 agcgtccgta tctcgtatcg ttatcgagcg tccggctaag agcatccgtg taaccattca
     5521 cactgctcgc ccgggtatcg ttatcggtaa aaaaggtgaa gacgtagaaa aactgcgtaa
     5581 ggtcgtagcg gacatcgctg gcgttcctgc acagatcaac atcgccgaag ttcgtaagcc
     5641 tgaactggac gcaaaactgg ttgctgacag catcacttct cagctggaac gtcgcgttat
     5701 gttccgtcgt gctatgaagc gtgctgtaca gaacgcaatg cgtctgggcg ctaaaggtat
     5761 taaagttgaa gttagcggcc gtctgggcgg cgcggaaatc gcacgtaccg aatggtaccg
     5821 cgaaggtcgc gtaccgctgc acactctgcg tgctgacatc gactacaaca cctctgaagc
     5881 gcacaccact tacggtgtaa tcggcgttaa agtgtggatc ttcaaaggcg agatcctggg
     5941 tggtatggct gctgttgaac aaccggaaaa accggctgct cagcctaaaa agcagcagcg
     6001 taaaggccgt aaataaggag cgtcgctgat gttacaacca aagcgtacaa aattccgtaa
     6061 aatgcataaa ggccgtaacc gcggtctggc gcagggtacg gatgttagct tcggcagctt
     6121 cggtctgaaa gctgttggcc gtggtcgtct gactgcccgt cagatcgaag cagcacgtcg
     6181 tgctatgacc cgtgcagtta agcgtcaagg taagatctgg atccgtgtgt tcccggacaa
     6241 accgatcact gaaaagccgc tggcagtgcg tatgggtaaa ggtaaaggta acgtggagta
     6301 ttgggttgcc ttgattcagc cgggtaaagt cctgtatgaa atggacggtg ttccggaaga
     6361 gctggcccgt gaagcattca agctggcagc agcgaaactg ccgattaaaa ccacctttgt
     6421 aactaagacg gtgatgtaat gaaagcaaaa gagctgcgtg agaagagcgt tgaagagctg
     6481 aacaccgagc tgctgaacct gctgcgtgag cagttcaacc tgcgtatgca ggctgcaagt
     6541 ggccagctgc aacagtctca cctgttgaag caagtgcgtc gcgatgtcgc acgcgttaag
     6601 actttactga acgagaaggc gggtgcgtaa tgaccgataa aatccgtact ctgcaaggtc
     6661 gcgttgttag cgacaaaatg gagaaatcca ttgttgttgc tatcgaacgt tttgtgaaac
     6721 acccgatcta cggtaaattc atcaagcgta cgaccaaact gcacgtacat gacgagaaca
     6781 acgaatgcgg tatcggtgac gtggttgaaa tccgcgaatg ccgtccgctg tccaagacta
     6841 aatcctggac gctggttcgc gttgtagaga aagcggttct gtaatacagt acactctctc
     6901 gatacgaata aacggctcag aaatgagccg tttatttttt ctacccatat ccttgaagcg
     6961 gtgttataat gccgcgccct cgatatgggg atttttaacg acctgatttt cgggtctcag
     7021 tagtagttga cattagcgga gcactaaaat gatccaagaa cagactatgc tgaacgtcgc
     7081 cgacaactcc ggtgcacgtc gcgtaatgtg tatcaaggtt ctgggtggct cgcaccgtcg
     7141 ctacgcaggc gtaggcgaca tcatcaagat caccatcaaa gaagcaattc cgcgtggtaa
     7201 ggtcaaaaaa ggtgatgtgc tgaaggcggt agtggtgcgc accaagaagg gtgttcgtcg
     7261 cccggacggt tctgtcattc gcttcgatgg taatgcttgt gttcttctga acaacaacag
     7321 cgagcagcct atcggtacgc gtatttttgg gccggtaact cgtgagcttc gtagtgagaa
     7381 gttcatgaaa attatctctc tggcaccaga agtactctaa ggagcgaatc atggcagcga
     7441 aaatccgtcg tgatgacgaa gttatcgtgt taaccggtaa agataaaggt aaacgcggta
     7501 aagttaagaa tgtcctgtct tccggcaagg tcattgttga aggtatcaac ctggttaaga
     7561 aacatcagaa gccggttccg gccctgaacc aaccgggtgg catcgttgaa aaagaagccg
     7621 ctattcaggt ttccaacgta gcaatcttca atgcggcaac cggcaaggct gaccgtgtag
     7681 gctttagatt cgaagacggc aaaaaagtcc gtttcttcaa gtctaacagc gaaactatca
     7741 agtaatttgg agtagtacga tggcgaaact gcatgattac tacaaagacg aagtagttaa
     7801 aaaactcatg actgagttta actacaattc tgtcatgcaa gtccctcggg tcgagaagat
     7861 caccctgaac atgggtgttg gtgaagcgat cgctgacaaa aaactgctgg ataacgcagc
     7921 agcagacctg gcagcaatct ccggtcaaaa accgctgatc accaaagcac gcaaatctgt
     7981 tgcaggcttc aaaatccgtc agggctatcc gatcggctgt aaagtaactc tgcgtggcga
     8041 acgcatgtgg gagttctttg agcgcctgat cactattgct gtacctcgta tccgtgactt
     8101 ccgtggcctg tccgctaagt ctttcgacgg tcgtggtaac tacagcatgg gtgtccgtga
     8161 gcagatcatc ttcccagaaa tcgactacga taaagtcgac cgcgttcgtg gtttggatat
     8221 taccattacc actactgcga aatctgacga agaaggccgc gctctgctgg ctgcctttga
     8281 cttcccgttc cgcaagtaag gtagggttac taaatggcta agcaatcaat gaaagcacgc
     8341 gaagtaaaac gcgtagcttt agctgataaa tacttcgcga aacgcgctga actgaaagcg
     8401 atcatctctg atgtgaacgc ttccgacgaa gatcgttgga acgctgttct caagctgcag
     8461 actctgccgc gtgattccag cccgtctcgt cagcgtaacc gctgccgtca aacaggtcgt
     8521 ccgcatggtt tcctgcggaa gttcgggttg agccgtatta aggtccgtga agccgctatg
     8581 cgcggtgaaa tcccgggtct gaaaaaggct agctggtaat tgtcaccaat tgaatcacgg
     8641 gagtaaagac agatgagcat gcaagatccg atcgcggata tgctgacccg tatccgtaac
     8701 ggtcaggccg cgaacaaagc tgcggtcacc atgccttcct ccaagctgaa agtggcaatc
     8761 gccaacgtgc tgaaggaaga aggttttatt gaagatttta aagttgaagg cgacaccaag
     8821 cctgaactgg aacttactct gaagtatttc cagggcaaag ctgttgtaga aagcattcag
     8881 cgtgtcagcc gcccaggtct gcgcatctat aaacgtaaag atgagctgcc gaaagttatg
     8941 gcgggtctgg gtatcgcagt tgtttctacc tctaaaggtg ttatgactga tcgtgcagcg
     9001 cgccaggctg gtcttggtgg cgaaattatc tgctacgtag cctaatcgga ggaaaaaatg
     9061 tctcgtgttg ctaaagcacc ggtcgttgtt cctgccggcg ttgacgtaaa aatcaacggt
     9121 caggttatta cgatcaaagg taaaaacggc gagctgactc gtactctcaa cgatgctgtt
     9181 gaagttaaac atgcagataa taccctgacc ttcggtccgc gtgatggtta cgcagacggt
     9241 tgggcacagg ctggtaccgc gcgtgccctg ctgaactcaa tggttatcgg tgttaccgaa
     9301 ggcttcacta agaagctgca gctggttggt gtaggttacc gtgcagcggt taaaggcaat
     9361 gtgattaacc tgtctctggg tttctctcat cctgttgacc atcagctgcc tgcgggtatc
     9421 actgctgaat gtccgactca gactgaaatc gtgctgaaag gcgctgataa gcaggtgatc
     9481 ggccaggttg cagcggatct gcgcgcctac cgtcgtcctg agccttataa aggcaagggt
     9541 gttcgttacg ccgacgaagt cgtgcgtacc aaagaggcta agaagaagta aggtaacact
     9601 atggataaga aatctgctcg tatccgtcgt gcgacccgcg cacgccgcaa gctccaggag
     9661 ctgggcgcaa ctcgcctggt ggtacatcgt accccgcgtc acatttacgc acaggtaatt
     9721 gcaccgaacg gttctgaagt tctggtagct gcttctactg tagaaaaagc tatcgctgaa
     9781 caactgaagt acaccggtaa caaagacgcg gctgcagctg tgggtaaagc tgtcgctgaa
     9841 cgcgctctgg aaaaaggcat caaagatgta tcctttgacc gttccgggtt ccaatatcat
     9901 ggtcgtgtcc aggcactggc agatgctgcc cgtgaagctg gccttcagtt ctaaggtaga
     9961 ggtgtaagat ggctcacatc gaaaaacaag ctggcgaact gcaggaaaag ctgatcgcgg
    10021 taaaccgcgt atctaaaacc gttaaaggtg gtcgtatttt ctccttcaca gctctgactg
    10081 tagttggcga tggtaacggt cgcgttggtt ttggttacgg taaagcgcgt gaagttccag
    10141 cagcgatcca gaaagcgatg gaaaaagccc gtcgcaatat gattaacgtc gcgctgaata
    10201 acggcactct gcaacaccct gttaaaggtg ttcacacggg ttctcgcgta ttcatgcagc
    10261 cggcttctga aggtaccggt atcatcgccg gtggtgcaat gcgcgccgtt ctggaagtcg
    10321 ctggggttca taacgttctg gctaaagcat atggttccac caacccgatc aacgtggttc
    10381 gtgcaactat tgatggcctg gaaaatatga attctccaga aatggtcgct gccaagcgtg
    10441 gtaaatccgt tgaagaaatt ctggggaaat aaaccatggc aaagactatt aaaattactc
    10501 aaacccgcag tgcaatcggt cgtctgccga aacacaaggc aacgctgctt ggcctgggtc
    10561 tgcgtcgtat tggtcacacc gtagagcgcg aggatactcc tgctattcgc ggtatgatca
    10621 acgcggtttc cttcatggtt aaagttgagg agtaagagat gcgtttaaat actctgtctc
    10681 cggccgaagg ctccaaaaag gcgggtaaac gcctgggtcg tggtatcggt tctggcctcg
    10741 gtaaaaccgg tggtcgtggt cacaaaggtc agaagtctcg ttctggcggt ggcgtacgtc
    10801 gcggtttcga gggtggtcag atgcctctgt accgtcgtct gccgaaattc ggcttcactt
    10861 ctcgtaaagc agcgattaca gccgaagttc gtctgtctga cctggctaaa gtagaaggcg
    10921 gtgtagtaga cctgaacacg ctgaaagcgg ctaacattat cggtatccag atcgagttcg
    10981 cgaaagtgat cctggctggc gaagtaacga ctccggtaac tgttcgtggc ctgcgtgtta
    11041 ctaaaggcgc tcgtgctgct atcgaagctg ctggcggtaa aatcgaggaa taagtagcag
    11101 atggctaaac aaccgggatt agattttcaa agtgccaaag gtggcttagg cgagctgaaa
    11161 cgcagactgc tgtttgttat cggtgcgctg attgtgttcc gtattggctc ttttattccg
    11221 atccctggta ttgatgccgc tgtacttgcc aaactgcttg agcaacagcg aggcaccatc
    11281 attgagatgt ttaacatgtt ctctggtggt gctctcagcc gtgcttctat ctttgctctg
    11341 gggatcatgc cgtatatttc ggcgtcgatc attatccagc tgctgacggt ggttcaccca
    11401 acgttggcag aaattaagaa agaaggggag tctggtcgtc gtaagatcag ccagtacacc
    11461 cgttacggta ctctggtgct ggcaatattc cagtcgatcg gtattgctac cggtctgccg
    11521 aatatgcctg gtatgcaagg cctggtgatt aacccgggct ttgcattcta cttcaccgct
    11581 gttgtaagtc tggtcacagg aacgatgttc ctgatgtggt tgggcgaaca gatcactgaa
    11641 cgaggtatcg gcaacggtat ttcaatcatt atcttcgccg gtattgtcgc gggactcccg
    11701 ccagccattg cccatactat cgagcaagcg cgtcaaggcg acctgcactt cctcgtgttg
    11761 ctgttggttg cagtattagt atttgcagtg acgttctttg ttgtatttgt tgagcgtggt
    11821 caacgccgca ttgtggtaaa ctacgcgaaa cgtcagcaag gtcgtcgtgt ctatgctgca
    11881 cagagcacac atttaccgct gaaagtgaat atggcggggg taatcccggc aatcttcgct
    11941 tccagtatta ttctgttccc ggcgaccatc gcgtcatggt tcgggggcgg tactggttgg
    12001 aactggctga caacaatttc gctgtatttg cagcctgggc aaccgcttta tgtgttactc
    12061 tatgcgtctg caatcatctt cttctgtttc ttctacacgg cgttggtttt caacccgcgt
    12121 gaaacagcag ataacctgaa gaagtccggt gcatttgtac caggaattcg tccgggagag
    12181 caaacggcga agtatatcga taaagtaatg acccgcctga ccctggttgg tgcgctgtat
    12241 attaccttta tctgcctgat cccggagttc atgcgtgatg caatgaaagt accgttctac
    12301 ttcggtggga cctcactgct tatcgttgtt gtcgtgatta tggactttat ggctcaagtg
    12361 caaactctga tgatgtccag tcagtatgag tctgcattga agaaggcgaa cctgaaaggc
    12421 tacggccgat aattggtcgc ccgagaagtt acggagagta aaaatgaaag ttcgtgcttc
    12481 cgtcaagaaa ttatgccgta actgcaaaat cgttaagcgt gatggtgtca tccgtgtgat
    12541 ttgcagtgcc gagccgaagc ataaacagcg ccaaggctga ttttttcgca tatttttctt
    12601 gcaaagttgg gttgagctgg ctagattagc cagccaatct tttgtatgtc tgtgcgtttc
    12661 catttgagta tcctgaaaac gggcttttca gcatggaacg tacatattaa atagtaggag
    12721 tgcatagtgg cccgtatagc aggcattaac attcctgatc ataagcatgc cgtaatcgca
    12781 ttaacttcga tttatggcgt cggcaagacc cgttctaaag ccatcctggc tgcagcgggt
    12841 atcgctgaag atgttaagat cagtgagctg tctgaaggac aaatcgacac gctgcgtgac
    12901 gaagttgcca aatttgtcgt tgaaggtgat ctgcgccgtg aaatcagcat gagcatcaag
    12961 cgcctgatgg atcttggttg ctatcgcggt ttgcgtcatc gtcgtggtct cccggttcgc
    13021 ggtcagcgta ccaagaccaa cgcacgtacc cgtaagggtc cgcgcaaacc gatcaagaaa
    13081 taatcggggt gattgaataa tggcaaaggc accaattcgt gcacgtaaac gtgtaagaaa
    13141 acaagtctct gacggcgtgg ctcatatcca tgcttctttc aacaacacca tcgtgactat
    13201 cactgatcgt cagggtaacg cgttgggttg ggcaacagcc ggtggttccg gtttccgtgg
    13261 ttctcgcaaa tccactccgt ttgcagctca ggttgcagca gagcgttgcg ctgacgccgt
    13321 gaaagaatac ggcatcaaga atctggaagt tatggttaaa ggtccgggtc caggccgcga
    13381 atctactatt cgtgctctga acgccgcagg tttccgcatc actaacatta ctgatgtgac
    13441 tccgatccct cataacggtt gtcgtccgcc gaaaaaacgc cgcgtataac gcctcgtttt
    13501 ccaggtttgt tggagaaaga aaatggcaag atatttgggt cctaagctca agctgagccg
    13561 tcgtgagggc accgacttat tccttaagtc tggcgttcgc gcgatcgata ccaagtgtaa
    13621 aattgaacaa gctcctggcc agcacggtgc gcgtaaaccg cgtctgtctg actatggtgt
    13681 gcagttgcgt gaaaagcaaa aagttcgccg tatctatggt gtgctggagc gtcagttccg
    13741 taactactac aaagaagcag cacgtctgaa aggcaacacc ggtgaaaacc tgttggctct
    13801 gctggaaggt cgtctggaca acgttgtata ccgtatgggc ttcggtgcca ctcgtgcaga
    13861 agcacgtcag ctggttagcc ataaagcaat tatggtaaac ggtcgtgttg ttaacatcgc
    13921 ttcttatcag gttagtccga atgacgttgt aagcattcgt gagaaagcga agaagcagtc
    13981 tcgcgtgaaa gccgctctgg agctggctga gcagcgtgaa aagccaacct ggctggaagt
    14041 tgatgctggc aagatggaag gtacgtttaa gcgtaagccg gagcgttctg atctgtctgc
    14101 ggacattaac gaacacctga tcgtcgagct ttactccaag taaagcttag taccaaagag
    14161 aggacacaat gcagggttct gtgacagagt ttctaaaacc gcgcctggtt gatatcgagc
    14221 aagtgagttc gacgcacgcc aaggtgaccc ttgagccttt agagcgtggc tttggccata
    14281 ctctgggtaa cgcactgcgc cgtattctgc tctcatcgat gccgggttgc gcggtgaccg
    14341 aggttgagat tgatggtgta ctacatgagt acagcaccaa agaaggcgtt caggaagata
    14401 tcctggaaat cctgctcaac ctgaaagggc tggcggtgag agttcagggc aaagatgaag
    14461 ttattcttac cttgaataaa tctggcattg gccctgtgac tgcagccgat atcacccacg
    14521 acggtgatgt cgaaatcgtc aagccgcagc acgtgatctg ccacctgacc gatgagaacg
    14581 cgtctattag catgcgtatc aaagttcagc gcggtcgtgg ttatgtgccg gcttctaccc
    14641 gaattcattc ggaagaagat gagcgcccaa tcggccgtct gctggtcgac gcatgctaca
    14701 gccctgtgga gcgtattgcc tacaatgttg aagcagcgcg tgtagaacag cgtaccgacc
    14761 tggacaagct ggtcatcgaa atggaaacca acggcacaat cgatcctgaa gaggcgattc
    14821 gtcgtgcggc aaccattctg gctgaacaac tggaagcttt cgttgactta cgtgatgtac
    14881 gtcagcctga agtgaaagaa gagaaaccag agttcgatcc gatcctgctg cgccctgttg
    14941 acgatctgga attgactgtc cgctctgcta actgccttaa agcagaagct atccactata
    15001 tcggtgatct ggtacagcgt accgaggttg agctccttaa aacgcctaac cttggtaaaa
    15061 aatctcttac tgagattaaa gacgtgctgg cttcccgtgg actgtctctg ggcatgcgcc
    15121 tggaaaactg gccaccggca agcatcgctg acgagtaacc ggatcacagg ttaaggtttt
    15181 actgagaagg ataaggtcat gcgccatcgt aagagtggtc gtcaactgaa ccgcaacagc
    15241 agccatcgcc aggctatgtt ccgcaatatg gcaggttcac tggttcgtca tgaaatcatc
    15301 aagacgactc tgcctaaagc gaaagagctg cgccgcgtag ttgagccgct gattactctt
    15361 gccaagactg atagcgttgc taatcgtcgt ctggcattcg cccgtactcg tgataacgag
    15421 atcgtggcaa aactgtttaa cgaactgggc ccgcgtttcg cgagccgtgc cggtggttac
    15481 actcgtattc tgaagtgtgg cttccgtgca ggcgacaacg cgccgatggc ttacatcgag
    15541 ctggttgatc gttcagagaa agcagaagct gctgcagagt aatctgaagc aacgtaaaaa
    15601 aacccgcccc ggcgggtttt tttatacccg tagcatcccc acttatctac aatagctgta
    15661 ctctttttgt tcatcccctg gagtatttat gtggttactt gaccagtggg cagagcgcca
    15721 tatagcagaa gcgcaagcga aaggtgagtt tgataacctg gcaggtagcg gcgaaccatt
    15781 gatactggat gatgattctc atgtgccacc ggaattacgt gcggggtatc gcttgctgaa
    15841 gaatgccggt tgcttaccgc cagaacttga gcaacggagg gaagcaatcc agcttctgga
    15901 tatcctcaaa ggtatccgtc acgatgatcc gcaatatcaa gaggttagcc gtcgattgtc
    15961 attactggaa ttgaagctgc gacaagctgg attgagtacc gattttttac gcggcgatta
    16021 tgctgacaag ttgttgaata aaatcaacga taactagtgg agtatgtatg tatcgcattg
    16081 gtgagctggc aaaaatggcg gaagtaacac ccgacacgat tcgttattac gaaaagcagc
    16141 agatgatgga gcatgaagtg cgtaccgaag gtgggtttcg cctgtatacc gaaagcgatc
    16201 tccagcgatt gaaatttatc cgccatgcca gacaactagg tttcagtctg gagtcgatcc
    16261 gcgagttgct gtcgatccgt atcgatcctg aacaccatac ctgtcaggag tcaaaaggca
    16321 ttgtgcagga aagattgcag gaagtcgaag cacggatagc cgagttgcag agtatgcagc
    16381 gttccttgca acgccttaac gatgcctgtt gtgggaccgc ccatagcagt gtttattgct
    16441 cgattcttga agctcttgaa caaggggcga gtggcgttaa gagtggctgt tgattttttg
    16501 cactggcagg attacactca cgccgttaaa taaccaactg gagtttttat gagccgatat
    16561 cagcatacta aagggcagat aaaggataat gcgatagaag cattactaca tgatccctta
    16621 ttccgacagc gcgtagagaa aaataagaag gggaaaggca gttacatgcg aaaaggaaaa
    16681 catggcaatc gggggaactg ggaggccagt ggcaagaaag taaatcactt ttttaccact
    16741 ggtcttctgc tttcaggggc ttgttaagag cggttattct gctctttcag caaatcacga
    16801 atttctgtca gtaatacttc ttctttagtt ggtgctggtg cggctgctgg ttcttctttt
    16861 ttccgattca gtttgttgat tagcttaatc gccataaaga tggcaaaggc cacaatcaga
    16921 aaatcaaaga cgttttgaat gaagacaccg taatgcatca caacagcagg gatatccccc
    16981 tgcgcatcgc gtagcgtgac agcaaactgt ttaaagtcga tcccgccaat taataagccc
    17041 agtggtggca tgatgatatc ggcaaccagt gaagatacaa tcttcccgaa tgccgcaccg
    17101 ataatgacac ccaccgccaa atccaccacg ttcccgcgca tcgcaaattc gcgaaattct
    17161 ttaataatgc tcatgttatt ctccctatga agccgacaac cataagtcta acaaatgtta
    17221 agccattttc ctgccaggaa tgaatttaat taatccttga taatcaaagg gaaataataa
    17281 ggcgtcatta gacgccttat taattacaag aagaaagggc ttggctggaa gagtctttcg
    17341 acgtcggtaa taaatttttt atctgtgagg aacataatta cgtgatcgcc ttgctcaatg
    17401 cgcagattgt cattggcaat catcacgtcg tttccacgta ccaccgctcc aataatcgtt
    17461 cctggcggta gcttgatttc gtcaatgact ctgccgacaa cgcgtgaggt gctttcatca
    17521 ccgtgagcaa cggcttcaat agcttctgct acgccgcggc gcaatgagga aacaccaaca
    17581 atatctgctt ttcgcacatg gctaagcaac gcagaaatag ttgcttgttg tggtgaaatc
    17641 gcaatatcga tcacgctccc ctgaaccaga tccacataag cgcgacgctg gatcaatacc
    17701 atcacctttt tcgcacccat acgtttggca agcatggcgg acatgatatt ggcctcgtca
    17761 tcgttggtga cggcaataaa cagatcaact tgatcgatat gttcttcggc cagcagttct
    17821 tgatccgacg catcaccaaa aaagacgatc gtattctgca acttttccgc cagttcggca
    17881 gcgcgctgct gattacgttc gatgagttta acgctgtaat ctttttccag acgacgcgcc
    17941 agccctgcac cgatattacc gccaccaacc agcatgatcc gcttatacgg tttttccagt
    18001 cgctgtaatt cactcatcac cgcgcggata tgctgtgaag cggcaataaa gaacacttca
    18061 tcaccagcct caacaatggt cgaaccttgc ggacgaatgg ggcgatcgtg gcggaaaatt
    18121 gctgccacac gagtatcgat atgtggcata tgttcgcgca tggtcgaaag tgcattacca
    18181 atcagcgggc cgccataata ggctttaacc acagcgaggc tgactttacc ctcagcgaag
    18241 ttcaccacct gcaatgcgcc gggatactca atcagtcggt aaatattatc gataaccaac
    18301 tgctctggtg cgatcagatg atcaatcggt acagcatctg aatgaaatag cttatcggca
    18361 tcgcgtacgt agtctggtga gcggatacga gcgatgcgat taggggtgtt gaaaagtgag
    18421 taggctacct ggcaggcaac catattggtt tcatctgaac tggttacagc aaccagcata
    18481 tcggcgtcgt cggcacctgc ctcccgcaat acgcgtggat gagagccatg cccctgcacg
    18541 actcgcaggt caaatttatc ctgcaaggtt cgcagacgct caccgttggt atcgacaaca
    18601 gtgatatcgt tgttctcgcc aaccaggttt tccgccagtg tgccgccaac ctggccggca
    18661 cccagaatga taattttcat cagtcgcgac ccgttatctc atcacttttt gattagctta
    18721 gcgtaaaaga agccgtcgcc ctcttcggca ccaggcagat tttgtttacc cggttgctct
    18781 ggtgttcctg tttcgcaaag ttcggcatca gcggtacgtt gcaaaaaggc tttaatctgc
    18841 aggctattct cttccggtaa caccgaacag gtggcataga ccagggttcc accggatttt
    18901 aaatgcggcc aaatggcgtc gagaatttca gactgcaatt gcgcgagttc tgggatatcg
    18961 cgatcgcgac gtaaccattt aatatccgga tggcgacgaa tcacaccggt tgctgaacaa
    19021 ggcgcatcta ataaaatgcg atcaaactgt tgctcgccac accattggga agggtaacgg
    19081 ccatcacctt gtttcacggt tgccttcata ccaaggcgtt ttaaattgtc gtaaacgcga
    19141 gagaggcgct gttcgtcaat atcaaccgca acaacctgcg cttctggtgc cacttcaagg
    19201 atatgcgttg ttttaccgcc gggggcggca caaagatcca gaatgtgttc accgttttgt
    19261 ggcgcaagcc aggtcatgca accttgtgct gatgcatcct gaacggtaac ccatccgtct
    19321 tcaaaaccag gtagcgcatg aacaggtgca ggtgtttcca gccgtacagc atcagggtaa
    19381 tccgcatgcg ggaaaccttt cattcctgct tcatccagca atgcaagcca ggtgtcgcgg
    19441 gaatgatgcg tacgattaat acgcagccac attggcggac gctggttatt ggcttcgacg
    19501 atggattgcc actgctctgg atacgctttt tgcagacgct tcagcaacca ggaaggatgc
    19561 agataacgtg catcactggt attaaactcg gctaataact cttcttgctg acgctggaac
    19621 tggcgtaata caccgttaat caacccttta agttgcggac gcttaattgc gatagcgcct
    19681 tcaaccgttt cagccagcgc agcatgaggt ggaatgcggg tataaagcag ttgatacaaa
    19741 ccaaccataa tcaggtaatg cacggtccgc tgtttgccgg tcatcggacg ggccattgac
    19801 ttattaatca gccagtctaa ctgcgaaagc gtacgcagta cgccaaagca caactcttga
    19861 agaagtgctt tgtctttatc ggaaactttt tgctggagcg gtggcagaat gttgcttaat
    19921 gattgccctt gctcgacgac ttgttcaacg gcctgggccg ccatgctacg taaattacgt
    19981 tgttttttca taagtataaa aatgcccggc aagaccgggc ttagaagagt ggactatcag
    20041 accagacggt tgcccggaac aaaccattcc cgacgagagt tcaggaggtc ttgcgcgctc
    20101 atcgctttct tacccgcagg ttgtaacgag agcaggttca ggatgccatc accagtcgca
    20161 acctgaatgc cttgtttgtt ggcttcaagg atcgttcctg gtgcagcgtt ggttgccgta
    20221 tcaatgaccg atgctttcca gactttaacc ggctgtcctt caatttccac ccagctcatt
    20281 ggccatggat tgaaagcgcg aatgcagcgt tcaagctgtg ctgccgaaag tgaccagtca
    20341 atacgcgctt cttctttact caacttctcg gcgtaagtga caagagtttc gtcctgaact
    20401 tctggtttcg ccgtgccgtc tgccaattgt ttcaacgtgg tgataagccc ttgtgggcca
    20461 agctctgcca gcttgtcgta cagcgtacca ctggtatctt ctgcagtaat cgggcaggag
    20521 agcttataga gcatgtcacc ggtgtctaaa ccaacatcca tttgcataat ggtcacacca
    20581 gtttctgcat cacccgccca tagtgagcgt tggattggtg cagcaccgcg ccagcgtggc
    20641 agcagtgaac catgaacgtt gatacagcca agacgcggca tctccagcac tgctttaggc
    20701 agaattaagc cataggcgac gacaaccata acatcagcct gtagatcggc gaccagttgc
    20761 tggttttctt gtggacgcag ggaaacaggt tgaaaaacgg gcagaccttt ttcctcagcc
    20821 agaactttaa ccgggctggg catcagtttt ttaccgcgtc ctgccggtcg gtctggctgg
    20881 gtgaacacgc caacgacgtt atgaccagaa gacaacagcg cgtcgagatg acgcgctgca
    20941 aagtcaggtg tacccgcaaa aataatacgt agtgattctg acacgttagt tcttatcctt
    21001 aagcccgggc tttcagacga tccagttttt caactttctg acgaatacgt tgttgtttca
    21061 gcggtgacag ataatccata aacagtttgc cgaccaggtg atccatctca tgctgaatac
    21121 agatggctaa cagaccatct gcctccagtt caaatggttt accgtcgcgg tccagggcgc
    21181 gaattttaac tttctctgcg cgtggcacta aagcacgttg ttcagggatc gacaggcaac
    21241 cttcttcaat gcctgtttcg ccgctttttt ctaaaagctc cggattgatt aacaccagcc
    21301 gttcgtcacg gttttccgaa acatcaataa caatgatacg ttgatggata tcaacctggg
    21361 ttgccgccag gccaatacct tcttctgcgt acatcgtctc gaacatatca tcgacgatac
    21421 gctgaatttc tgcattcact tcttctaccg gtttagcaac tttgcgaagc cgctcgtccg
    21481 gaatatgtaa cacttgcaaa actgacataa atctccagag atgtgttcag gagttagaaa
    21541 gattatttct tctattctag acaaatcccc ctctgattga cagcatcact gaccaatcgc
    21601 aaagattgct aaggctgctt atggcaggga gataaggatg gtcgatacag aaatttggct
    21661 gcgtttaatg agtatcagca gcttgtacgg cgatgatatg gtccgtatag ctcactggct
    21721 ggcaagacag tcgcatattg atgcggttgt attgcagcaa acagggctta cattgcggca
    21781 ggcacaacgc tttctttcat ttccgcggaa gagtatcgaa agctcacttt gttggttgga
    21841 gcaacccaac catcatttaa tccctgcgga cagcgaattt tatcctcctc aacttctggc
    21901 gacgacagat taccccggtg cactgtttgt tgaaggagaa ctgcacgcgc tgcattcatt
    21961 tcagcttgcc gtagtgggga gtcgggcgca ttcatggtat ggcgagcgat ggggacgatt
    22021 attttgcgaa actctggcga agcatggagt gacaattacg agtggactgg cgcgtggaat
    22081 cgatggtgta gcgcataaag cagccttaca ggtaaatggc gtcagcattg ctgtattggg
    22141 gaatggactt aataccattc atccccgccg tcatgcccga ctggctgcca gtctgcttga
    22201 acaggggggc gctctcgtct cggaatttcc cctcgatgtt ccaccccttg cttacaattt
    22261 cccacgaaga aatcgcatta tcagtggtct aagtaaaggt gtactggtgg tggaagcggc
    22321 tttgcgtagt ggttcgctgg tgacagcacg ttgtgcgctt gagcaggggc gagaagtttt
    22381 tgccttgcca ggtccaatag ggaatccggg aagcgaaggg cctcactggt taataaaaca
    22441 aggtgcgatt cttgtgacgg aaccggaaga aattctggaa aacttgcaat ttggattgca
    22501 ctggttgcca gacgcccctg aaaattcatt ttattcacca gatcaggaag acgtggcatt
    22561 gccatttcct gagctcctgg ctaacgtagg agatgaggta acacctgttg acgtcgtcgc
    22621 tgaacgtgcc ggccaacctg tgccagaggt agttactcaa ctactcgaac tggagttagc
    22681 aggatggatc gcagctgtac ccggcggcta tgtccgattg aggagggcat gccatgttcg
    22741 acgtactaat gtatttgttt gaaacctata ttcacacaga agctgagttg cgtgtggatc
    22801 aagacaaact tgaacaggat cttaccgacg caggatttga tcgagaagat atctacaatg
    22861 ccctgctttg gctggaaaag cttgcggatt atcaggaagg actggcagaa ccgatgcaac
    22921 tggcctctga tccactctcc atgcgtattt atacccctga ggagtgtgaa agactggatg
    22981 ccagctgtcg tggttttctg cttttccttg agcaaattca ggtgcttaac cttgaaaccc
    23041 gtgaaatggt gatagagcga gtgctcgcgc tggataccgc agagttcgac ctggaagacc
    23101 tgaaatgggt aatcctgatg gtgttgttca atattccggg ctgcgaaaat gcgtaccagc
    23161 aaatggaaga attactcttt gaagtgaatg aaggtatgct gcattaatca tttctttaat
    23221 tcagcataag ttgttatggc gaaatcagca ctgttcacgg tgcgtaataa tgagtcctgc
    23281 ccaaagtgcg gggctgaact ggttattcga tccgggaaac acggtccgtt tcttggatgc
    23341 tcacagtatc cggcgtgtga ctacgtccgt cctctgaaat cttcagcgga tggacatatc
    23401 gtcaaagttc tggaggggca ggtttgccct gcatgtggcg caaatctggt attacgccag
    23461 ggacgctttg gtatgtttat tggttgcatt aactaccctg aatgcgaaca taccgaactt
    23521 atcgataaac cggacgaaac agcaattaca tgtccccaat gccggacggg ccatctggtc
    23581 cagcgccgct cccgttatgg caaaacattt cactcctgtg atcgctaccc ggagtgtcaa
    23641 tttgccatta acttcaaacc catagctgga gaatgccctg agtgtcatta tccgctactc
    23701 atcgaaaaga aaaccgcgca gggtgtaaaa cacttttgtg ccagtaaaca atgtggaaag
    23761 ccggtttcgg cggaataata acgtgaataa taacctgcaa ggagacgcta tcgcagctgc
    23821 gatagatgtt ctcaatgaag aacgtgtcat cgcctatcca acggaagccg ttttcggtgt
    23881 tgggtgcgat cctgatagcg aaacagcagt gatgcgactg ttggagttaa aacagcgtcc
    23941 ggttgataag gggctgattt taatcgcagc aaattacgag cagcttaaac cctatattga
    24001 tgacaccatg ttgactgacg tgcagcgtga aaccattttt tcccgctggc caggtcctgt
    24061 cacctttgtc tttcccgcgc ctgcgacaac accgcgctgg ttgacgggcc gctttgattc
    24121 gcttgctgta cgagtcaccg accatccgtt ggtggttgct ttgtgccagg cttatggtaa
    24181 accgctggtt tctaccagtg ccaacttgag tggattgcca ccttgtcgaa cagtagacga
    24241 agttcgcgca caatttggcg cggcgttccc ggttgtgcct ggtgaaacgg gggggcgttt
    24301 aaatccttca gaaatccgcg atgccctgac gggtgaactg tttcgacagg ggtaacataa
    24361 tggaaaccta tgctgttttt ggtaatccga tagcccacag caaatcgcca ttcattcatc
    24421 agcaatttgc tcagcaactg aatattgaac atccctatgg gcgcgtgttg gcacccatca
    24481 atgatttcgt caacacactg aacgctttct ttagtgctgg tggtaaaggt gcgaatgtga
    24541 cggtgccttt taaagaagag gcttttgcca gagcggatga gcttactgaa cgcgcagcgt
    24601 tggctggtgc tgttaacacc ctcaagcggt tagaagatgg acgcttgctg ggtgacaata
    24661 ccgatggtgt aggcttgtta agcgatctgg aacgtctgtc ttttatccgt cctggtttac
    24721 gtattctgct catcggcgca ggtggagcat ctcgcggcgt actactgcca cttctttccc
    24781 tggactgtgc ggtgacaatc actaatcgga cggtatcccg agcggaagag ttggctaaat
    24841 tgtttgcgca cactggcagt attcaggcgt tgggtatgga cgaactggaa ggtcatgagt
    24901 ttgatctcat tattaatgcc acatccagtg gcatcagtgg tgatattccg gcgatcccgt
    24961 catcgctcat tcatccaggc atttattgct atgacatgtt ctatcagaaa ggaaaaactc
    25021 cttttttggc atggtgtgag cagcgaggct caaagcctac tgctgatggt ttaggaatgc
    25081 tggtggcaca ggcggctcat gccttccttc tttggcatgg cgttctgcct gatgtagaac
    25141 cagttataaa gcaattgcag gaggaattgt ccgcgtgaat caggccatcc agtttccgga
    25201 cagagaagag tgggtcgaga ataaaaaatg tgtatgtttt cccgctctcg tgaatggcat
    25261 gcaactgaca tgtgcgatct ctggcgatag cctggcgtat cgctttacag gagatacgcc
    25321 agagcagtgg ttagcgagtt ttcgtcagca tcgctgggac ttggaagaag aagcggaaaa
    25381 cttaattcag gaacaaagtg aagatgatca aggctgggtc tggttaccct gatccagata
    25441 ttcgtccttc catttcacgt aattattcgc ggaatagcgt aacccagcct tctcttcatc
    25501 acttaacggg cggatctgtt tgacgggact accgagatac agatatccgc tctccagccg
    25561 tttattttgt gggaccagac tacccgcacc aatcatcaca tcatcttcta ctattgcgcc
    25621 atcaagtaaa attgagccca tcccaaccaa aactcgattg ccaatggtgc agccgtggag
    25681 catcaccttg tgaccaacag tgacatcttc gccaatggtt aatgggttgc catctgggtt
    25741 gtacgaggat ttatgagtga catgcaacat actgccatcc tggatattgg tgcgtgctcc
    25801 gatctgtaca taatgtacat ctccacgaat cacaacgagc ggccagatcc ccacatcatc
    25861 agccagacga acgtcaccaa tcacgacact gctatcgtcg atcattacgc gctgaccgat
    25921 ttgtggaaaa agatcgcggt atgggcgtaa aacatcagac atacttacct cagcaataaa
    25981 tgatttacta atgactttgg gggcattatt ggccttgtgc aagtctttta gtatgcaaaa
    26041 aagcaccgtt ttgtgtgcga atgcagcaaa aagggtgaaa aaacaacaaa cagaaaaaaa
    26101 gatcaaaaaa gtacttgtgc aaaaaattgg gatccctata atgcgcctcc gttgagacga
    26161 caacgtgaaa cacttcacaa gatggtcgta acaacgaaga gaaaaaatcc tg
//
LOCUS       DAZKIF010000064        26023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZKIF010000064 DAZKIF010000000
VERSION     DAZKIF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.064"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            358..1497
                     /gene="wza"
                     /locus_tag="ACHOTW_002614"
     CDS             358..1497
                     /gene="wza"
                     /locus_tag="ACHOTW_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP9844697.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1503..1946
                     /gene="wzb"
                     /locus_tag="ACHOTW_002615"
     CDS             1503..1946
                     /gene="wzb"
                     /locus_tag="ACHOTW_002615"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP9844698.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1949..4111
                     /gene="wzc"
                     /locus_tag="ACHOTW_002616"
     CDS             1949..4111
                     /gene="wzc"
                     /locus_tag="ACHOTW_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP9844699.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4204..5043
                     /gene="wcaA"
                     /locus_tag="ACHOTW_002617"
     CDS             4204..5043
                     /gene="wcaA"
                     /locus_tag="ACHOTW_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP9844700.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5046..5534
                     /gene="wcaB"
                     /locus_tag="ACHOTW_002618"
     CDS             5046..5534
                     /gene="wcaB"
                     /locus_tag="ACHOTW_002618"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP9844701.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5531..6748
                     /gene="wcaC"
                     /locus_tag="ACHOTW_002619"
     CDS             5531..6748
                     /gene="wcaC"
                     /locus_tag="ACHOTW_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP9844702.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6723..7940
                     /gene="wcaD"
                     /locus_tag="ACHOTW_002620"
     CDS             6723..7940
                     /gene="wcaD"
                     /locus_tag="ACHOTW_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP9844703.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7951..8697
                     /gene="wcaE"
                     /locus_tag="ACHOTW_002621"
     CDS             7951..8697
                     /gene="wcaE"
                     /locus_tag="ACHOTW_002621"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP9844704.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8713..9261
                     /gene="wcaF"
                     /locus_tag="ACHOTW_002622"
     CDS             8713..9261
                     /gene="wcaF"
                     /locus_tag="ACHOTW_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP9844705.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9290..10411
                     /gene="gmd"
                     /locus_tag="ACHOTW_002623"
     CDS             9290..10411
                     /gene="gmd"
                     /locus_tag="ACHOTW_002623"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9844706.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10414..11379
                     /gene="fcl"
                     /locus_tag="ACHOTW_002624"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10414..11379
                     /gene="fcl"
                     /locus_tag="ACHOTW_002624"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP9844707.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11382..11861
                     /gene="gmm"
                     /locus_tag="ACHOTW_002625"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11382..11861
                     /gene="gmm"
                     /locus_tag="ACHOTW_002625"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP9844708.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11858..13081
                     /gene="wcaI"
                     /locus_tag="ACHOTW_002626"
     CDS             11858..13081
                     /gene="wcaI"
                     /locus_tag="ACHOTW_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP9844709.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13084..14520
                     /gene="cpsB"
                     /locus_tag="ACHOTW_002627"
     CDS             13084..14520
                     /gene="cpsB"
                     /locus_tag="ACHOTW_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP9844710.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14713..16083
                     /gene="cpsG"
                     /locus_tag="ACHOTW_002628"
                     /gene_synonym="manB"
     CDS             14713..16083
                     /gene="cpsG"
                     /locus_tag="ACHOTW_002628"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP9844711.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16138..17532
                     /gene="wcaJ"
                     /locus_tag="ACHOTW_002629"
     CDS             16138..17532
                     /gene="wcaJ"
                     /locus_tag="ACHOTW_002629"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP9844712.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17534..19012
                     /gene="wzxC"
                     /locus_tag="ACHOTW_002630"
     CDS             17534..19012
                     /gene="wzxC"
                     /locus_tag="ACHOTW_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP9844713.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19192..20472
                     /gene="wcaK"
                     /locus_tag="ACHOTW_002631"
     CDS             19192..20472
                     /gene="wcaK"
                     /locus_tag="ACHOTW_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP9844714.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20469..21689
                     /gene="wcaL"
                     /locus_tag="ACHOTW_002632"
     CDS             20469..21689
                     /gene="wcaL"
                     /locus_tag="ACHOTW_002632"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP9844715.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21700..23094
                     /gene="wcaM"
                     /locus_tag="ACHOTW_002633"
     CDS             21700..23094
                     /gene="wcaM"
                     /locus_tag="ACHOTW_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP9844716.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23252..24247
                     /locus_tag="ACHOTW_002634"
     CDS             23252..24247
                     /locus_tag="ACHOTW_002634"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFP9844717.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24490..25383
                     /gene="galF"
                     /locus_tag="ACHOTW_002635"
     CDS             24490..25383
                     /gene="galF"
                     /locus_tag="ACHOTW_002635"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP9844718.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            25702..>26023
                     /locus_tag="ACHOTW_002636"
     CDS             25702..>26023
                     /locus_tag="ACHOTW_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9844719.1"
                     /translation="MNKETVSIIMPVYNGAKTIISSVESIIHQSYQDFVLYIIDDCST
                     DDTFSLINSRYKNNQKIRILRNKTNLGVAESRNYGIEMATGKYISFCDADDLWHEKKL
                     ERQIE"
BASE COUNT         6482 a         6403 c         6990 g         6148 t
ORIGIN      
        1 ctgtaacact tcctcctgca ttattggaga gccaatattc aattaccata atgtcacctt
       61 atattcggaa gttaatttcc gcaataaccc tttctcatca acgattgtat gcagagaggg
      121 tttgaaaaat taagctaaat cagtgtattg gtagctaaaa agccaggggc ggtagcgtgt
      181 ctggatgcct gaaagaccag tctgaattat tctgtcaata gcctgcggat gaaatcaatt
      241 tttttaggac tgatgccagt taaattttta ttcaattaac tgcaaggtaa atgtgcatgg
      301 cgacattgct attaatagtg cacaggataa ttactctgcc aaagtgataa ataaacaatg
      361 atgaaatcca aaatgaaatt gatgccatta ttggtgtcag taaccttgat aagcggttgc
      421 acagtacttc cgggcagcaa tatgtcgacg atgggcaaag acgtcatcaa acagcaggac
      481 gctgatttcg atctcgacaa aatggtgaat gtttatccgc tgaccccgcg cctgattgac
      541 caattacgcc cacgcccgaa tgtagcgcgc cccaatatga cgctggaaag tgagatcgcg
      601 aattaccagt atcgcgtcgg gccgggggac gttcttaatg tcaccgtctg ggatcacccg
      661 gaactcacca cgccagccgg tcagtaccgc agctccagcg acaccggcaa ctgggtacag
      721 cctgacggca ctatgtttta cccgtatatc ggcaaggttc acgtagtcgg gaaaacgctc
      781 gctgaaatcc gcagtgatat taccgggcgc ttagcgacgt acatcgctga cccgcaggtg
      841 gacgttaata tcgccgcctt ccgctcacaa aaggcctata tctcaggtca ggtgaataaa
      901 tctggtcaac aggcgatcac caacgtacca ctgaccattc tcgacgccat caacgccgca
      961 ggtggcctga ccgacaccgc tgactggcgc aacgtggtgc taacacacaa tggtcgtgaa
     1021 gagcgaattt ctttgcaggc gctgatgcaa aacggcgacc tcaatcagaa ccgactgctt
     1081 taccccggcg atattctcta cgtgccgcgt aatgatgatc tgaaagtgtt tgtgatgggt
     1141 gaagtgaaga aacagagcac cctgaaaatg gactttagcg gcatgaccct gaccgaagcc
     1201 ctgggcaatg ctgaaggcat cgacatgacc acctccaacg ccagcggcat ctttgtcatt
     1261 cgtccgctga aaggcgaggg cgggcgtaac ggcaaaattg ccaatatcta ccagctggat
     1321 atgtccgatg ccacgtcgct ggtgatggcg acagaattcc gcctgcaacc ttatgacgtg
     1381 gtgtatgtca ccaccgcccc ggtttcccgc tggaaccgtc tgatcaatca gttgctgcca
     1441 actattagcg gtgtccgtta catgacggat acagccagcg acattcataa ctggtaatcg
     1501 tcatgtttaa caacatctta gttgtctgtg tcggcaatat ttgccgttcc ccgacggcgg
     1561 aacgcttact gcaacgttat cacccggagc tgaaagtgga gtccgctgga ctcggcgcgc
     1621 tggtcggtaa gggcgctgat cctaccgcta tcagcgtcgc cgcagaacat caactgtctc
     1681 tggaaggcca ctgtgcacga caaatcagcc gccgcctgtg tcgcaactat gacctgattt
     1741 tgaccatgga aaagcgccat atcgaacgct tatgcgagat ggcaccggag atgcgcggca
     1801 aagtgatgct gtttggtcac tgggataacg aatgtgaaat ccccgatccg tatcgcaaaa
     1861 gccgggaaac gtttgcagcg gtgtacacat tacttgaacg gtctgcccgc cagtgggcgc
     1921 aggcattgaa cgcagagcag gtataagaat gacagaaaaa gtaaaacaac atgccgctcc
     1981 ggtaacgggc agtgatgaaa tcgatattgg tcgcctggtc ggcaccgtca ttgaagcgcg
     2041 ctggtgggtg attggcatca ccgctgtatt cgccctttgt gccgtggttt acaccttctt
     2101 cgccacgccg atttatagtg ccgacgcact ggtacaaatc gagcaaagca gcggcaattc
     2161 gttagtgcag gatatcggat cggcgttagc caacaaaccg cctgcatcgg acgccgagat
     2221 ccagttgatt cgttcgcgcc tggtgcttgg taaaacggtg gatgatctcg acctcgatat
     2281 tgcggtgagc aaaaacacgt tcccgatttt cggtgcgggc tgggatcgcc tgatgggacg
     2341 ccagaacgag acggtgaaag tgactacctt taaccgtccg aaagagatgg aggatcaggt
     2401 gtttacgctt aatgtgctgg acaacaaaaa ctacaccctg agcagcgatg gcggctttag
     2461 cgcccgtggg caagcgggcc agatactgaa aaaagaaggc gtcacgctga tggttgaagc
     2521 cattcacgcc cgcccgggca gtgagtttac cgtcaccaaa tactccacgc tggggatgat
     2581 caatcaactg caaaacagcc tgacggtaac ggagaacggc aaagacgcag gcgtactgag
     2641 cctgacttat accggtgaag atcgcgaaca gatccgcgac attcttaaca gcatcgcccg
     2701 taactatcag gaacaaaata ttgagcgcaa atcggcggaa gcgtcgaaaa gcctcgcttt
     2761 cctcgcgcaa cagttaccgg aagtacgtag ccgccttgat gttgccgaaa acaaactgaa
     2821 tgccttccgt caggataaag attctgttga tctgccgctg gaagcgaaag cggtgctcga
     2881 ttcgatggtg aacatcgacg cccagttgaa cgaactgacc tttaaagagg cggaaatctc
     2941 caagctgtac accaaagttc accccgcgta ccgcacgctg ctggagaaac gtcaggcgct
     3001 ggaagacgaa aaagccaaac ttaatggtcg cgtaacggcg atgccgaaaa cccagcagga
     3061 aattgtccgt ctgacccgcg atgtcgagtc tggtcagcag gtctatatgc aactgctgaa
     3121 taaagagcag gagctgaaaa tcaccgaggc cagcaccgtc ggcgatgtgc gcattgttga
     3181 cccggcaatc actcagcctg gtgtgctaaa accgaagaaa gggctgatta tccttggggc
     3241 gattatcctt ggcctgatgc tctctatcgt gggggtgctg ctgcgctcgt tgtttaatcg
     3301 cggcatcgaa agcccgcagg tgctggaaga acacggtatc agcgtctatg ccagcatccc
     3361 gctgtcggag tggcagaaag cgcgcgatag cgtcaaaacc atcaaaggga ttaaacgcta
     3421 taaacagagc cagctactgg cggtggggaa tccaaccgat ctggcgattg aagccatccg
     3481 cagccttcgt accagtttgc acttcgcgat gatgcaggcg cagaacaatg tgttgatgat
     3541 gaccggggtt agcccgtcaa tcggtaaaac ctttgtctgc gccaacctgg cggcggtgat
     3601 cagccagacc aataaacgcg tgttgttgat cgactgcgat atgcgcaaag gctacaccca
     3661 tgagctgttg ggcactaata acgttaatgg cctgtcggaa attctgattg gtcagggcga
     3721 tattactaca gctgctaaac cgacctctat tgccaaattt gacctgatcc cgcgcggtca
     3781 ggtaccgcca aatccttctg aactgttgat gagcgaacgc tttgccgaac tggtgaactg
     3841 ggcgagtaaa aactacgacc tggtgttgat tgatacgccg ccgattctgg cagtgaccga
     3901 tgcggcaatt gttggtcgtc atgtcggaac cacgttaatg gtggcgcgtt atgcggtcaa
     3961 cacattgaaa gaagtggaaa ccagtctgag ccgctttgag caaaacggta ttccggtgaa
     4021 aggggtgatt ctgaactcca tcttccgccg cgccagcgcg tatcaggatt atggctatta
     4081 cgaatacgaa tataagtcgg atgcgaaata acgaggcctg cattcgcacc gccccgtagg
     4141 ccggataagg cgctcacgcc gcatccggca agcaaaccag ctcataagcc gggagtacaa
     4201 cctatgaaag acaatccgct gatctcaatc tatatgccga cctggaaccg ccaacaactg
     4261 gcgattcggg cgataaaatc ggtcttgcgc caggactaca gcaactggga gatgatcatc
     4321 gtggatgatt gctccacctc ctgggaacaa ctgcaacagt acgtcaccgc cctcaacgat
     4381 ccgcgtatta cctacattca caacgacatt aacagcgggg cgtgcgcggt acgtaaccag
     4441 gcgattatgc tggcgcaagg ggaatacatt accgggatcg atgacgatga tgaatggacg
     4501 cccaaccgcc tgagcgtctt cctcgcccat aaacagcaac tggtcactca cgcctttttg
     4561 tacgctaacg attacgtttg ccagggcgaa gtttattccc aaccggcaag cttaccactg
     4621 tatcccaaat cgccgtactc acgccgcctg ttctataaac gcaatatcat tggcaatcag
     4681 gtttttacct gggcatggcg tttcaaagag tgtttgttcg ataccgaact gaaagcggca
     4741 caggattacg acatcttcct gcggatggtg gtggagtatg gcgaaccgtg gaaagtggaa
     4801 gaggcgacgc agatcctgca tatcaatcac ggtgaaatgc agatcacctc gtcaccgaaa
     4861 aaattctccg gatacttcca cttttaccgc aagcacaaag acaaattcga ccgtgccagt
     4921 aaaaaatatc agctgtttac cctctaccag atccgcaata aacgtatgac ctggcgcact
     4981 ttgctgacgc tgctgtcggt gcgtaacggc aaacgtctgg ctgatggtat tcgggggcgc
     5041 taaatatgct ggaagatcta cgcgccaaca gctggagttt acgcccgtgc tgcatggttc
     5101 ttgcctatcg cgtcgctcat ttttgttcgg tgtggcgcaa aaagaacgtt ctcaacaatc
     5161 tttgggcggc cccgctgctg gtgctgtatc gcattatcac cgaatgcttt ttcggttatg
     5221 aaatccaggc tgccgcgacc attggccgcc gctttactat ccatcacggt tacgccgtag
     5281 tgatcaataa aaacgtggta gcgggggatg atttcaccat tcgccacggc gtcactatcg
     5341 gcaatcgtgg tgccgataac atggcatgtc cacacattgg caacggcgtc gaactcggtg
     5401 ccaacgtcat tattcttggt gatatcacgc ttggtaacaa cgtcaccgtg ggcgcgggca
     5461 gcgtggtgct cgactctgtc ccggacaacg cgctggtggt gggagaaaaa gcgcgagtga
     5521 aggtaattaa atgaatattc tgcaatttaa tgtgcgactg gcggaaggcg gggcggcagg
     5581 tgtggcgtta gatctccacc agcgtgcgct gcaacagggg ctggcgtcac attttgtcta
     5641 cggctacggc aaaggcggca aagagagcgt cagccatcag aactatccgc aggtcatcaa
     5701 acatacgccg cggatgaccg caatggcgaa tattgccctg tttcgtctgt ttaatcgcga
     5761 tctgtttggc aatttcaatg agttatatcg caccattact cgtacaccgg gtccggtggt
     5821 cctgcatttt catgtgctgc acagctactg gctaaatctt aagagcgtgg tgcgcttttg
     5881 cgaaaaggtg aaaaaccaca aaccggatgt cactctggtc tggacgctgc acgaccactg
     5941 gagcgttacc gggcgctgcg cctttaccga cggttgtgaa ggctggaaaa cgggctgcca
     6001 gaaatgcccg accttaaata actatccgcc ggtgaagatt gatcgggcgc accagctggt
     6061 ggcgggcaaa cgccagttat tccgtgagat gctggcgctg ggctgtcagt ttatttcccc
     6121 cagccagcat gtggctgacg ctttcaatag cctgtacggt ccagggcgtt gccggattat
     6181 caataacggt attgatatgg caaccgaggc gattctggcg gatttgcctc cggtgcgcga
     6241 aactcagggc aagccgaaaa tcgcggtggt agcgcatgac ctgcgttacg acggcaaaac
     6301 taaccagcaa ctggtacgcg agatgatggc gctgggcgac aaaatcgaac tgcatacctt
     6361 tggtaagttc tcgccgttca ccgctggcaa cgtggttaat cacggctttg aaactgacaa
     6421 gcgcaagttg atgagcgcgc tcaatcagat ggatgcgctg gtgttcagtt ctcgcgtcga
     6481 taactacccg ctgattttgt gtgaggcgtt atcaattggc gtgccggtga ttgccaccca
     6541 tagcgacgcg gcgcgtgaag tgttgcaaaa atccggcggt aaaaccgtca gcgaagaaga
     6601 ggtgctgcaa ctggtccagt taagcaaacc ggaaatcgcg caggcgttat ttggtaccac
     6661 gctggctgag ttcagccaac gcagccgcgc cgcctacagt ggacaacaga tgctggagga
     6721 gtatgtcaac ttctatcaga atctgtagct acctgctgct gccgctgatc tacctgctgg
     6781 ttaacgtcaa aatcacccag cttggcgaaa gtttccccat caccatcgtc acttttttac
     6841 ctgtcttgtt actgctgttt ttagaacgca tcagcgttaa aaaattgatg attgccttag
     6901 ggatttgcgc gggactcacg gcgtttaact acctgtttgg tcagtcgctg gatgccagta
     6961 aatacgtcac ttcaactatg ctgtttgtct atatcgtgat cattattggt atggtgtgga
     7021 gtattcgctt taaaacgatt tcgccacaca accatcgcaa gatattacgt ttcttttatc
     7081 tggtggtcgg gctggtggtg gcgctggcgg cggtggagat ggcgcaaatt atcctcaccg
     7141 gcggcagcag tattatggag tcgatttcga aatatctgat ttacagcaac agctatgtgc
     7201 tgaatttcat caaattcggc ggcaagcgca cgacagcact ttatttcgaa ccggcatttt
     7261 tcgctctggc attaatctca atttggctca gcatcaaaca gtttggtatc aaaacgccta
     7321 aaacagatgc tatgattctc gcagggataa tattatccgg atcgttttca ggggttatga
     7381 cctttatcct gttttatttg ctggagtggg catttcaata tctgaataaa gaggcgatta
     7441 agaaaaagtt accgttagca ttgatttctc tggcagtatt cctggttggt gtggtaattg
     7501 catttcctta tatttccacc cgtctgggcg atttaggtac ggaaggatcg tcatcgtatt
     7561 atcgtattgt cggtccgctg gtgatggtcg gttattcttt gacccatatt gacggtgtag
     7621 tcagatttgg ctcactttat gaatatgtcg catcattcgg aatatttaac ggtgcggatg
     7681 tcggaaaaac catagacaat ggtttgtatc tgctgattat ttatttttct tggttcgcgg
     7741 tgtttttatc gctgtggtac atggggaaag tgataaaaat gatgatcaac gcttttggtg
     7801 ataaccgcaa ttttcgcgtg caattatatc tttttactcc ggtatcgctg ttttttaccg
     7861 gttcgatatt tagcccggaa tatgcatttt taatcgtctg tccgtttatt ttgcgaaaag
     7921 cgttaaatat tacgaggtaa gaataagaac atgttgctta gcataatcac tgtcgcgttt
     7981 cgtaacctcg aagggatagt caaaacacat gcctcgctgg cgcatctggc gcaggcggaa
     8041 gatatcagct tcgaatggat tgttgtcgat ggcggttcca atgacggcac tcgtgagtac
     8101 ctggaaaatc tcaatggtat ctataaccta cgctttgtca gcgagccaga taacggtatc
     8161 tacgacgcca tgaacaaggg cattgcgatg gcacaaggca agttcgcgtt gtttctcaac
     8221 tcgggcgata tttttcatca ggatgccgca tattttgtcc gtaagttaaa aatgcaaaaa
     8281 gataacgtga tgatcaccgg cgatgcgctg ctggattttg gcgacgggca taaaattaaa
     8341 cgtagcgcca aaccgggctg gtatatttat cacagcctgc ccgccagtca tcaggcgata
     8401 tttttcccgg tatccggttt gaaaaaatgg cgttatgacc tggaatataa agtttcttct
     8461 gattacgcgc tggcagccaa aatgtataaa gcaggttatg catttaaaaa actcaatggc
     8521 ctggtgtctg aattttctat gggtggggta tctaccacca ataatatgga attgtgtgct
     8581 gacgcgaaaa aagtccaacg gcaaatatta catgtgcctg gcttttgggc tgaattatcc
     8641 tggcatttac gccagcgtac tacctcaaag acgaaagcct tatataacaa aagctgaata
     8701 taagggaaaa cgatgcagga tttaagcggt ttctcggtgc cgaaagggtt ccggggcggc
     8761 aacgctatta aagtgcaatt gtggtgggca gtacaggcaa caatatttgc ctggtcgcca
     8821 caagtattgt atcgctggcg ggcattttta ttacgtttat tcggcgcaaa aataggaaaa
     8881 aacgtagtta ttcgtccgtc agtaaaaatt acctatccgt ggaaattaac cttaggtgat
     8941 tacgtgtggg tcggcgatga cgtcaattta tataccctcg gtgaaataac cattggcgca
     9001 cattcggtga tatcgcaaaa aagttattta tgcaccggga gccacgacca tgcaagtcaa
     9061 catttcacca ttaacgccac gcctattgtg attggcgaga aatgctggct ggcaaccgat
     9121 gtttttgttg ccccaggcgt cacgattggc gacggcaccg tcgtgggtgc gcgaagcagt
     9181 gtttttaaat cgcttccggc aaatgtggtt tgccggggga atcccgcagt ggtgatacgc
     9241 aaacgcgttg aaactgaata aattcaaaaa aaatacagag gaataagaca tgtcaaaagt
     9301 cgctctcatc accggtgtaa ccggacaaga cggttcttac ctggcagagt ttctgctgga
     9361 aaaaggttac gaggtgcatg gtattaagcg tcgcgcatcg tcattcaaca ccgagcgcgt
     9421 ggatcacatt tatcaggatc cgcacacctg caacccgaaa ttccatctgc attatggcga
     9481 cctgagtgat acctccaacc tgacgcgtat tttgcgtgaa gtgcagccgg atgaagtgta
     9541 caacctgggc gcaatgagcc acgttgcggt ttcttttgag tcaccggaat ataccgctga
     9601 cgtcgacgcg atgggtacgc tgcgcctgct ggaggcgatc cgcttcctcg gtctggaaaa
     9661 gaaaacccgt ttctatcagg cttccacctc tgaactgtat ggtctggtgc aggaaattcc
     9721 gcagaaagag accacgccgt tctatccgcg ctctccgtat gcggtcgcca aactgtatgc
     9781 ctactggatc accgtcaact accgtgaatc ctacggcatg tatgcctgta acggtattct
     9841 cttcaaccat gaatccccgc gccgcggcga aaccttcgtt acccgcaaaa tcacccgcgc
     9901 aatcgccaac atcgcccagg ggctggagtc gtgcctgtac ctcggcaata tggattccct
     9961 gcgtgactgg ggccatgcca aagactacgt aaaaatgcag tggatgatgc tgcaacagga
    10021 acagccagaa gatttcgtta tcgcgaccgg cgttcagtac tccgtacgtc agttcgtaga
    10081 aatggcggca gcacagctgg gcatcaaact gcgctttgaa ggcacgggcg ttgaagagaa
    10141 gggcattgtg gtttccgtca ccgggcatga cgcgccgggt gttaaaccgg gtgatgtgat
    10201 tatcgccgtt gatccgcgtt acttccgtcc ggctgaagtt gaaacgctgc tcggcgaccc
    10261 gaccaaagcg cacgaaaaac tgggctggaa accggaaatc accctcagag agatggtgtc
    10321 tgaaatggtg gctaatgacc tcgaagcggc gaaaaaacac tctctgctga aatctcacgg
    10381 ctacgacgtg gcgatcgcgc tggagtcata agtatgagta gacaacgcat ttttattgct
    10441 ggccatcgcg ggatggtcgg ttccgccatc aggcggcagc tcgaacagcg cggtgatgtg
    10501 gaactggtat tacgcacccg cgacgagctg aacctgctgg acagccgcgc cgtgcatgat
    10561 ttctttgcca gcgaacgtat tgaccaggtc tatctggcgg ccgcgaaagt gggcggcatt
    10621 gttgccaaca acacgtatcc ggcggatttc atctatcaga acatgatgat tgagagcaac
    10681 atcattcacg ccgcgcatca gaacgacgtg aacaaactgc tgtttctcgg atcgtcctgc
    10741 atctacccga aactggcaaa acagccgatg gcagaaagcg agttattgca gggcacgctg
    10801 gagccgacca acgagcctta cgccattgcc aaaatcgccg ggatcaaact gtgcgaatca
    10861 tacaaccgcc agtacggacg cgattaccgc tcagtcatgc cgaccaacct gtatggcccg
    10921 catgacaact tccacccgag taattcgcat gtgatccctg cattgctgcg tcgcttccac
    10981 gaggcgacgg cacagaatgc accggacgtg gtggtgtggg gcagcggtac gccgatgcga
    11041 gaatttctgc acgtcgatga tatggcggcg gcgagcattc atgtcatgga gctggcgcat
    11101 gaagtctggc tggagaacac ccagccgatg ctgtcgcaca ttaacgtcgg cacgggcgtt
    11161 gactgcacta tccgcgagct ggcgcaaacc atcgccaaag tggtgggtta caaaggtcgg
    11221 gtggtttttg atgccagcaa accggatggt acgccgcgca aactgctgga tgtgacgcgc
    11281 ctgcatcagc ttggctggta tcacgaaatc tcactggaag cggggcttgc cagcacttac
    11341 cagtggttcc ttgagaatca agaccgcttt cgggggtaat gatgttttta cgtcaggaag
    11401 actttgccac ggtagtgcgc tccacaccgc ttgtctctct cgactttatt gtcgagaaca
    11461 gtcgcggcga gtttctgctt ggcaaaagaa ccaaccgccc ggcgcagggt tactggtttg
    11521 tgccgggagg gcgcgtgcag aaagacgaaa cgctggaagc cgcatttgag cggctgacga
    11581 tggcggaact ggggctgcgt ctgccgataa cagcaggcca gttttacggt gtctggcagc
    11641 acttttatga cgataacttc tccggcacgg atttcaccac tcactatgtg gtgctcggtt
    11701 ttcgcttcag agtagcggaa gaagaactgt tactgccgga tgagcagcat gacgattacc
    11761 gctggctgac gccggacgcg ctgctcgcca gtaacgatgt tcacgctaac agccgcgcct
    11821 attttctcgc tgagaagcgt gccggagtac ccggattatg aaaatactgg tctacggcat
    11881 taactactcg ccggagttaa ccggcatcgg caaatacacc ggcgagatgg tggaatggct
    11941 ggcggcgcaa ggtcatgagg tgcgggttat taccgcaccg ccttactacc cgcagtggca
    12001 agtgggcgag aactattccg cctggcggta caaacgagaa gagggggccg ctacggtgtg
    12061 gcgctgcccg ctgtacgtgc caaaacagcc gagcaccctg aaacgcctgt tgcatctcgg
    12121 cagttttgcc gtcagcagtt ttttcccgtt gatggcgcaa cgtcgctgga agccggatcg
    12181 cattatcggc gtagtgccaa cgctgttttg cacgccggga atgcgcctgc tggcgaaact
    12241 ctctggcgcg cgtaccgtgc tgcatattca ggattacgaa gtagatgcca tgctggggct
    12301 gggccttgcc ggaaaaggca aaggcggcaa agtggcacag ctggcaacgg cgttcgaacg
    12361 tagcggactg cataacgtcg ataacgtttc cacgatttcg cgttcgatga tgaataaagc
    12421 catcgaaaaa ggcgtggcgg cggaaaacgt catcttcttc cccaactggt cggaaatcgc
    12481 ccgttttcag catgttgcag acgccgatgt tgatgccctt cgtaaccagc ttggcctgcc
    12541 ggataacaaa aaaatcattc tttactccgg caatattggt gaaaagcagg ggctggaaaa
    12601 cgttattgaa gcagccgatc gcctgcgcga tgaaccgctg atttttgcca ttgtcgggca
    12661 gggcggcggc aaagcgcggc tggaaaaaat ggcgcagcag cgtggactgc gcaacatgca
    12721 atttttcccg ctgcaatcgt atgacgcttt acccgcactg ctgaagatgg gcgattgcca
    12781 tctggtggtg caaaaacgcg gcgcggcaga tgccgtattg ccgtcgaaac tgaccaatat
    12841 tctggcagta ggcggtaacg cggtgattac tgctgaagcc cacacagaac tgggacagct
    12901 ttgcgaaacc tttccgggca ttgcggtttg cgtagaaccg gaatcggttg aggcgctggt
    12961 ggcggggatt cgtcaggcgc tcctgctgcc caaacacaac acggtggcac gtgaatatgc
    13021 cgaacgcacg ctcgataaag agaacgtgtt acgtcaattt ataaatgata ttcggggata
    13081 attatggcgc agtcgaaact ctatccagtt gtgatggcag gtggctccgg tagccgctta
    13141 tggccgcttt cccgcgtact ttatcccaag cagtttttat gcctgaaagg cgatctcacc
    13201 atgctgcaaa ccaccatctg ccgcctgaac ggcgtggagt gcgaaagccc tgtggtgatt
    13261 tgcaatgagc agcaccgctt tattgttgcg gaacaactgc gtcaactgaa caaactcact
    13321 gagaacatta ttctcgaacc ggcagggcgt aacacagcac cggccattgc gctggcggcg
    13381 ctggcggcaa aacgtcatag cccggagaac gacccattaa tgctggtgct ggcggcggat
    13441 catgtgattg ccgatgaaga cgcattccgc gccgccgtgc gtaacgctat gccgtatgcc
    13501 gaagcgggca agctggtgac cttcggcatt gtgccggatc tacctgaaac cggatatggc
    13561 tatattcgtc gcggtgaagt gtcggcgggt gagcaggata cggtggcctt tgaagtggcg
    13621 cagtttgtcg aaaaaccaaa tctggaaacc gcgcaggcct atgtggcaag cggcgaatat
    13681 tactggaaca gcggtatgtt cctgttccgt gccggacgct atctcgaaga actgaaaaaa
    13741 tatcgcccgg atattctcga cgcctgtgaa aaagcgatga gcgccgtcga tccggatctc
    13801 gattttattc gcgtggatga agacgcgttt ctggcctgcc cggaagagtc ggtggattac
    13861 gcggtcatgg aacgtacggc agatgccgtt gtggtgccga tggatgcggg ctggagcgat
    13921 gtcggctcct ggtcctcatt atgggagatc agcgcccaca ccgccgaggg caacgtttgc
    13981 cacggcgatg tgattaatca caaaactgaa aacagctatg tgtatgccga atctggcctg
    14041 gtcaccaccg tcggggtgaa agatttggtg gtagtgcaga ccaaagatgc ggtgctgatt
    14101 gccgaccgta acgcggtgca ggatgtgaaa aaagtggtcg agcagatcaa agccgatggt
    14161 cgccatgagc atcgggtgca tcgcgaagtc tatcgcccgt ggggcaaata tgactctatc
    14221 gacgcgggcg accgctacca ggtgaaacgc atcaccgtga aaccgggcga aggcttgtcg
    14281 gtacagatgc accatcaccg cgcggaacac tgggtggttg tcgcgggaac ggcaaaagtc
    14341 accattgacg gtgatatcaa actgcttggt gaaaacgagt cgatttatat tccgctgggg
    14401 gcgacgcact gcctggaaaa cccggggaaa attccgctcg atttaattga agtgcgctcc
    14461 ggctcttatc tcgaagagga tgatgtggtg cgcttcgcgg atcgctacgg acgggtgtaa
    14521 acgtcgcatc aggcgctgat tgtcggatgc ggcgtgaacg ccttatccga cctacggttc
    14581 ggttttgtag gcctgataag acgcggcagc gtcgcatcag gcaatgaatg cgaaaccgcg
    14641 gtgtaaataa cgacaaaaac aaaattggcc gtttcggtca gggccaacta ttgcctgaaa
    14701 aagggtaacg atatgaaaaa attaacctgc tttaaagcct atgatattcg cgggaaatta
    14761 ggcgaagaac tgaatgaaga tattgcctgg cgcattggtc gtgcctatgg cgaatttctc
    14821 aaaccgaaaa ccattgtgtt aggcggtgat gcccgtctca ccagcgaaac cttaaaactg
    14881 gcgctggcaa aaggtttaca ggatgcgggc gttgacgtgc tggatattgg tatgtccggc
    14941 accgaagaga tctatttcgc cacgttccat ctcggcgtgg atggcggcat tgaagttact
    15001 gccagccata acccgatgga ttacaacggc atgaaactgg tgcgcgaagg ggctcgcccg
    15061 atcagcggcg ataccggact gcgcgatgtc cagcgtctgg cagaagccaa cgactttcct
    15121 cccgttgatg aaaccaaacg cggtcgctat cagcaaatca atctgcgtga cgcttacgtt
    15181 gatcacctgt tcggttatat caacgtcaaa aaccttacgc cgctcaagct ggtgatcaac
    15241 tccgggaacg gcgcggcggg tccggtggtg gacgccatcg aagcccgctt taaagccctc
    15301 ggcgcaccgg tggaattgat caaagtgcac aacacgcccg acggcaattt ccccaacggt
    15361 attcctaacc cgctgctgcc ggaatgtcgc gacgacaccc gcaatgcggt cattaaacac
    15421 ggcgcggata tgggcattgc ttttgatggc gattttgacc gctgtttcct gtttgacgaa
    15481 aaagggcagt ttatcgaggg ctactacatt gtcggtctgc tggcagaagc gttcctcgaa
    15541 aaaaatcccg gcgcgaagat catccacgat ccacgtctct cctggaatac cgttgatgtg
    15601 gtgactgccg caggcggcac cccggtaatg tcgaaaaccg gacacgcctt tattaaagaa
    15661 cggatgcgca aggaagatgc catctacggt ggcgaaatga gcgcccacca ttacttccgt
    15721 gattttgctt actgcgacag cggcatgatc ccgtggctgc tggtcgccga actggtgtgc
    15781 ctgaaaggaa agacgctggg cgaactggtg cgcgaccgga tggcagcgtt tccgtcaagc
    15841 ggtgagatca acagcaaact ggcgcaaccc gttgaggcga ttaaccgcgt ggaacagcat
    15901 tttagccgtg aggcgctggc ggtggatcgc accgatggca tcagcatgac ctttgccgac
    15961 tggcgcttta atctgcgctc ctccaacacc gaaccggtgg tgcggttgaa tgtggaatca
    16021 cgcggtgatg tgccgctgat ggaagcgcga acgcgaactc tgctgacgtt gctgaacgag
    16081 taatgtcgga tcttccctta ccccattgcg ggtaaggggc taataacagg aacaacgatg
    16141 acaaatctaa aaaagcgcga gcgagcgaaa accaatgcat cgttaatctc tatggtgcaa
    16201 cgcttttcag atatcaccat catgtttgcc ggactatggc tggtttgcga agtgagcgga
    16261 ctgtcattcc tctacatgca cctgttggtg gcgctgatta cgctggtggt gttccagatg
    16321 ctgggcggca tcaccgattt ttatcgctca tggcgcggtg ttcgggcagc gacagaattt
    16381 gccctgttgc tacaaaactg gaccttaagc gtgattttca gcgccggact ggtggcgttc
    16441 aacaatgatt tcgacacgca actgaaaatc tggctggcgt ggtatgggct gaccagtatc
    16501 ggactggtgg tttgccgttc atgtattcgc attggggcgg gctggctgcg taatcatggc
    16561 tataacaagc gcatggtcgc ggtggcgggg gatttagccg ccgggcaaat gctgatggag
    16621 agcttccgta accagccgtg gttagggttt gaagtggtgg gcgtttacca cgacccgaaa
    16681 ctgggcggcg tttctaacga ctgggcgggt aacctgcaac agctggtcga ggatgctaaa
    16741 gcaggcaaga ttcataacgt ctatatcgcg atgcaaatgt gcgacggcgc gcgagtgaaa
    16801 aaactggtcc atcaactggc ggacaccacc tgttcggtgc tgctgatccc cgatgtcttt
    16861 accttcaaca ttctccattc acgtcttgaa gagatgaacg gcgttccggt ggtgccgctt
    16921 tacgacacgc cgctttccgg ggttaaccgc ctgctcaaac gtgcggaaga cattgtgctg
    16981 gcgacgctta tcctgctgct gatctccccg gtgctgtgct gcattgcgct ggcggtgaaa
    17041 ctcagttcac ctgggccggt tattttccgc cagactcgct acggcatgga tggcaagccg
    17101 atcaaagtgt ggaagttccg ttccatgaaa gtgatggaga acgacaaagt ggtgacccag
    17161 gcgacgcaga acgatccgcg cgtcaccaaa gtggggaact ttctgcgccg cacctcgctg
    17221 gatgaattgc cgcagtttat caatgtgctg accgggggga tgtcgattgt cggtccacgt
    17281 ccgcacgcgg tggcgcataa cgaacagtat cgacagctca ttgaaggcta catgctgcgt
    17341 cataaggtga aaccgggcat taccggctgg gcgcagatta acggctggcg cggcgaaacc
    17401 gacacgctgg agaaaatgga aaaacgcgtc gagttcgacc ttgagtacat ccgcgaatgg
    17461 agcgtctggt tcgatatcaa aatcgttttc ctgacggtat tcaaaggatt cgttaacaaa
    17521 gcggcatatt gatatgagct tacgtgaaaa aaccatcagc ggcgcgaagt ggtcggcgat
    17581 tgccacggtg atcatcatcg gcctcgggct ggtgcagatg accgtgctgg cgcggattat
    17641 cgacaaccac cagttcggtt tgcttaccgt gtcgctggtg atcatcgcgc tggcagatac
    17701 gctttctgac ttcggtattg ctaactcgat tattcagcga aaagaaatca gccaccttga
    17761 actcaccacg ctgtactggc tgaacgttgg gctggggctt gtggtgtgtg tggcggtgtt
    17821 tttgttgagt gatctcattg gtgacgtgct gaacaacccg gacctggcac cgttgattaa
    17881 aacattgtcg ctggcgtttg tggttatccc ccacgggcaa cagttccgcg cgttgatgca
    17941 aaaggagctg gagttcaaca aaatcggcat gatcgaaacc agcgcggtac tggcgggctt
    18001 cacttttacg gtggttagcg cccatttctg gccgctggcg atgaccgcga tcctcggtta
    18061 tctggtcaat agtgcggtga gaacgctgct gtttggctac tttggccgca aaatttatcg
    18121 ccccggtctg catttctcgc tggcgtctgt tgcgccgaac ttacgctttg gtgcctggct
    18181 gacggcggac agcatcatca actatctcaa taccaacctt tcaacgctgg tgctggcgcg
    18241 tattctcggc gcgggcgtgg cagggggata caacctggcg tacaacgtgg ccgttgtgcc
    18301 gccgatgaag ctgaacccga tcatcacccg tgtgttgttt ccggcattcg ccaaaattca
    18361 ggacgatacc gaaaagctgc gcgtcaactt ctacaagctg ctgtcggtgg tggggattat
    18421 caactttccg gcgctgctcg ggctgatggt ggtgtcaaat aactttgtac cgctggtatt
    18481 tggcgaaaag tggaacagca ttattccggt gctgcaattg ctgtgtgtgg tgggtctgct
    18541 gcgctccgta ggtaacccga ttggttcgct gctgatggcg aaagcgcggg tcgatatcag
    18601 ctttaaattc aacgtattca aaacatttct gtttattccg gcgattgtta ttggtgggca
    18661 gatggcgggc gcaatcggcg tcacgctcgg tttcctgctg gtgcagatta tcaacaccat
    18721 tctgagttac ttcgtgatga ttaaaccggt tcttggttcc agttatcgcc agtacatcct
    18781 gagtttgtgg ctgccgtttt atctctcgct gccgacgctg gtggtcagtt atgtgctggg
    18841 cattgtgctg aaagggcaac tggcgctggg gatgctgctg gcggtgcaaa tagccgcggg
    18901 cgtgctggcg tttgtggtga tgattgtgct gtcgcgccat ccgctggtgg tggaagtgaa
    18961 gcgccagttt tgtcgcagcg aaaaaatgaa aatgctttta cgggcggggt gagttgttgt
    19021 tttcggttcc gacaacggtt gccgggtgca gcgtaaaaca ccttatccat cctacggttc
    19081 tgtgctcggg tcttgtaggc ctgataagac gcgccagcgt cgcatcaggc aaaaaacggc
    19141 aacgaacaca acaaacacag cctcacctgg catcaaattt atgaggaaaa aatgaaatta
    19201 cttattctgg gcaaccacac ttgcggcaat cgtggcgaca gcgccatctt gcgcggctta
    19261 cttgatgcca tcaacatcct caatccacat accgaagtgg acgtgatgag ccgctatccg
    19321 gtcagttctt cctggctgct caaccgcccg gtaatgggtg atccgctgtt ccttcaaatg
    19381 aaacaacaca acagcgcggc gggcgttgtc gggcgcgtta aaaaagtcct ccgtcgccgc
    19441 tatcagcacc aggtattgct ctcacgcgtc accgacactg gcaagctgcg caatatcgcc
    19501 atcgcccagg gattcaccga cttcgtgcgc ctactgtcag gttacgacgc cattattcag
    19561 gtcggcggat cgttttttgt cgatctctac ggcgtgccgc agtttgaaca tgcactttgc
    19621 acatttatgg cgaaaaagcc gctgtttatg attggtcaca gcgtcggtcc cttccaggat
    19681 gagcaattta accaactggc gaactacgtt tttggtcact gcgacgcgct gatcttgcgc
    19741 gaatcggtca gcctcgatct aatgaaacgc agcaatatca ccactgcaaa agttgaacat
    19801 ggcgtcgata ccgcgtggct ggtcgatcac cacacagaag acttcaccgc cagctatgcc
    19861 gtccaacact ggctggacgt tgccgcacaa cagaaaacgg tggcaattac cctgcgcgaa
    19921 ctggcaccgt tcgacaaacg cctcggcacc actcagcaag tgtatgaaaa agcctttgcc
    19981 ggggtggtca atcgcattct cgacgaaggc tatcaggtca ttgcgctctc cacctgtacg
    20041 ggtatcgaca gctataacaa agacgaccgc atggtggcgc tcaacctgcg ccagcacatc
    20101 agcgatcctg cccgttatca cgtagtgatg gatgaactta acgatctgga aatgggcaaa
    20161 attctcggtg cctgtgaact caccgtcggt acgcgcctgc actccgccat tatctcaatg
    20221 aactttgcta ctccggcgat tgccatcaac tatgaacata aatccgccgg ggttatgcag
    20281 cagctgggac taccggagat ggcaattgat atccgtcatt tattagacgg cagcctgcaa
    20341 gcgatggttg cggatacctt aggccagctt ccggcgctga acgcacgact tagcgaagcc
    20401 gtcagtcgtg agcgtcagac gggaatgcag atggtgcagt ccgtactgga acgcatcggg
    20461 gaggtgaaat gaaggtcggc ttcttcttat tgaaatttcc gctgtcgtca gaaaccttcg
    20521 ttcttaacca aatcactgcg tttattgata tgggctttga agtggagatt gtcgcgctgc
    20581 aaaaaggcga cacacaaaac acccacgtgg catggactaa atacaacctt gccgccagaa
    20641 cccgctggtt acaggacgaa cctacgggca aagtggcgaa actgcgccac cgagccagtc
    20701 agaccttgcg cggcattcat cgtaaaaata cctggcaggc gctcaacctc aaacgctatg
    20761 gtgccgagtc gtggaacctg attttgtctg ccatttgcgg ccaggtcgca acaccgtttc
    20821 gcgccgatgt gttcatcgct cattttggcc ctgcgggggt aaccgcagca aaactacgcg
    20881 aactgggtgt cattcacggc aaaattgcca ctattttcca cggcattgat atttccagtc
    20941 gggaagtgct caaccattac actcccgaat atcaacaact gtttcgacgt ggcgacctga
    21001 tgctaccgat aagcgatctg tgggccggaa ggctgcaaaa aatgggctgc ccgagggaaa
    21061 aaatcgccgt atcgcgcatg ggcgtagata tgacgcgctt tagcccgcgt cccgtgaaag
    21121 cgcccgcaac accgctggag attatttccg tcgcacgctt aaccgagaaa aaaggtctgc
    21181 atgtggcgat tgaagcctgc cggcagttga aagagcaggg cgtggcattt cgctatcgca
    21241 tcctcggcat tggcccgtgg gaacgacgcc tgcgcacgct catcgaacaa tatcaactgg
    21301 aagatgtggt ggagatgccg ggctttaaac cgagccatga agtgaaagcg atgctcgacg
    21361 acgcggatgt cttcctgttg ccatcggtta caggtgcgga tggcgatatg gaaggcattc
    21421 cggtggcgct gatggaggcg atggcggtcg gcattccggt ggtttctact ctgcatagcg
    21481 gaataccgga actggtggag gctgacaaat ccggctggct ggtgcctgag aacgatgctc
    21541 gcgcactggc gcaacgactg gcggcgttta gccaactgga caccgacgaa ttggctccgg
    21601 tcgtcaaacg cgcgcgcgaa aaagttgaac acgattttaa ccagcaggtg atcaatcgag
    21661 aactcgccag cttgctgcag gctttataga ggagaacgta tgccatttaa aaaactctcc
    21721 cgacgcacct tcctgacggc aagctcggcg cttgccttcc ttcatacccc tttcgctcgc
    21781 gcacttcccg cccgacaaag cgttaatatc aacgactaca accctcacga ctggatcgcc
    21841 tcatttaaac aagccttcag cgaagggcaa acggttgtcg tgcctgctgg attcgtttgt
    21901 gacaatatca acaccggcat cttcattcct cctggcaaaa cgttacacat ccttggaagc
    21961 ctgcgcggca acggcagagg gcgatttgtc ttacaggacg gcagccaggt gacaggggag
    22021 gagggcggca gtatgcataa catcaccctg gatgtgcgtg gttctgactg caccatcaaa
    22081 gggctggcga tgagcggctt tggtccggta acgcagattt atatcggcgg caaaaacaaa
    22141 cgggtcatgc gcaacctgac catcgataac ctgaccatta gccacgctaa ttacgccatc
    22201 ttacgccagg gatttcataa ccagattatc ggtgccaaca tcaccaattg taagttcagc
    22261 gacttacagg gcgatgccat tgaatggaac gtggcgatta acgaccgcga tattttgatc
    22321 tccgaccatg tcatcgagcg catcaactgt actaacggca aaatcaactg gggcattggc
    22381 ataggccttg cgggaagcac ttacgataat aattacccgg aagatcaggc agtgaaaaac
    22441 tttgtcgtgg cgaatatcac gggatcggat tgtcggcagt tgatacatgt tgaaaatggt
    22501 aaacattttg ttattcgtaa tatcaaagcc cgcaatatca cgcaggattt cagtaagaaa
    22561 gcaggtattg ataacgccac agtcgctatt tacggttgtg acaatttcgt gattgataat
    22621 attgaaatga ttaatagcgc cgggatgtta atcggctatg gggtaattaa aggcaattat
    22681 ctctcgattc cgcaaaattt ccgagtgaat aatattcaac tggataacac ccatcttgct
    22741 tataaattgc gcggcctcca aatctctgcc gggaatgccg tctcctttgt ggcactaact
    22801 aacattgaga tgaagcgtgc gtcgctggag ttacacaaca aaccgcaaca tctttttctg
    22861 cgtaatatca aagtgatgca ggaatcctct gttggacccg cattgattat gaacttcgac
    22921 atgcgcaaag acgttcgagg cgtctttatg gcgaaagaag aaacactgct gtctcttgca
    22981 aatgttcatg cggtgaatga gaaaggacaa agctccgtcg atatcgacag gattaatcac
    23041 catattgtta atgtggaaaa gattaacttt agattgccgg aacggagaga gtagatttgc
    23101 gaccattcat aaacttactg gatataatgt tctaaaagat tataatcctc acactaactg
    23161 ttaaagctgg ggctgggcag cattgactac tcagggaagg tactcatctt tatcttgtgt
    23221 ttcttacgta acaaccagac taaagtgatt tatgaacgat aacgttttgc tcataggagc
    23281 ttccggattc gtaggaaccc gactacttga aacggcaatt gctgacttta atatcaagaa
    23341 cctggacaaa cagcagagcc acttttatcc agaaatcaca cagattggtg atgttcgtga
    23401 tcaacaggca ctcgaccagg cgttagccgg ttttgacact gttgtactac tggcagcgga
    23461 acaccgcgat gacgtcagcc ctacttctct ctattatgat gtcaacgttc agggtacccg
    23521 caatgtgctg gcggccatgg aaaaaaatgg cgttaaaaat atcatcttta ccagttccgt
    23581 tgctgtttat ggtttgaaca aacacaaccc tgacgaaaac catccacacg accctttcaa
    23641 ccactacggc aaaagcaagt ggcaggcgga ggaagtgctg cgtgaatggt ataacaaagc
    23701 accaacagaa cgttcattaa ctatcatccg tcctaccgtt atcttcggtg aacgcaaccg
    23761 cggtaacgtc tataacttgc tgaaacagat cgctggcggc aagtttatga tggtgggcgc
    23821 agggactaac tataagtcca tggcttatgt tggaaacatt gttgagttta tcaagtacaa
    23881 actgaagaat gttgccgcag gttacgaggt ttataactac gttgataagc cagacctgaa
    23941 catgaaccag ttggttgctg aagttgaaca aagcctgaac aaaaagatcc cttctatgca
    24001 cttgccttac ccactaggaa tgctgggtgg atattgcttt gatatcctga gcaaaattac
    24061 gggcaaaaaa tacgctgtca gctctgtgcg cgtgaaaaaa ttctgcgcaa caacacagtt
    24121 tgacgcaacg aaagtgcatt cttcaggttt tgtggcaccg tatacgctgt cgcaaggtct
    24181 ggatcgaact ctgcagtatg aattcgtcca tgccaaaaaa gacgacataa cgtttgtttc
    24241 tgagtaatgc agacgagtca gagaaaaaag gaccaggttg gactacgatt attcggaaag
    24301 aggtactgat taaattatca gtatgtggat ttccgagtaa tccgagtaac ccagatgctg
    24361 atggtgtgta gtgttatcgt aaggtttaat caaagttatg cggaactaag ctttggtata
    24421 tagccataac gtttggctaa cctatggcaa taatttacgc actatcagcc acttagaggt
    24481 aggaaaagaa tgacgaattt aaaagcagtt ataccggtag caggtcttgg gatgcatatg
    24541 ttgcctgcca ctaaggcgat tcccaaagag atgctaccga tcgtcgacaa gccaatgatt
    24601 cagtacattg ttgacgagat tgtggctgca gggatcaaag aaatcctcct ggtaactcac
    24661 gcgtccaaga acgcagtcga aaaccacttc gacacctctt atgaattaga atctctcctt
    24721 gaacagcgcg tgaagcgtca actactggcg gaagtacaat ctatctgtcc gccgggcgtg
    24781 accattatga acgtgcgtca gggtgaacct ttaggtttag gccactccat tttatgtgcg
    24841 cgacctgcca ttggtgacaa tccatttgtc gtggtgctgc cagacgttgt gatcgacgac
    24901 gccagcgccg acccgctacg ctacaacctt gctgccatga ttgcgcgctt caatgaaacg
    24961 ggacgcagcc aggtgctggc aaaacgtatg ccgggcgacc tctctgaata ctccgtcatc
    25021 cagaccaaag aaccgctgga tcgtgaaggc aaagtcagcc gcattgttga atttatcgaa
    25081 aagccggatc agccgcagac gctggactca gacatcatgg ccgttggtcg atatgtgctt
    25141 tctgccgata tttggccgga actggaacgt actcagcctg gtgcatgggg acgtattcag
    25201 ctgactgatg ccattgctga gctggcaaaa aaacaatccg ttgatgcaat gctgatgact
    25261 ggcgacagtt acgactgcgg taaaaaaatg ggctatatgc aggcgtttgt gaagtatgga
    25321 ctacgcaact tgaaagaagg ggcgaagttc cgcaaaggta tcgagaagct gttaagcgaa
    25381 taatgaaaat ctgaccgaat gtaacggttg ataaggaaat aatatcggca gtgaaaaact
    25441 atagatcaat aaatatcact gctgttgagc ttttataatt aactatgcta ccaatgaata
    25501 aatagtgtgc agatatgtat tttttgtttg gtagtcttgt ttctccctct gaataataag
    25561 ataataaacg cttggagatg atttttttaa catggattat catttcgtac actggtagcg
    25621 attaagccag gggcggtagc gtgcataaat tttaatgctt atcaaaacta ttagcattaa
    25681 aaatatataa gaaattctca aatgaacaaa gaaaccgttt caataattat gcccgtttac
    25741 aatggggcca aaactataat ctcatcagta gaatcaatta tacatcaatc ttatcaagat
    25801 tttgttttgt atatcattga cgattgtagc accgatgata cattttcatt aatcaacagt
    25861 cgatacaaaa acaatcagaa aataagaata ttgcgtaaca agacaaattt aggtgttgca
    25921 gaaagtcgaa attatggaat agaaatggcc acggggaaat atatttcttt ttgtgatgcg
    25981 gatgatttgt ggcacgagaa aaaattagag cgtcaaatcg aag
//
LOCUS       DAZKIF010000065        25848 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZKIF010000065 DAZKIF010000000
VERSION     DAZKIF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.065"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            313..4296
                     /gene="ehaA"
                     /locus_tag="ACHOTW_002637"
     CDS             313..4296
                     /gene="ehaA"
                     /locus_tag="ACHOTW_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFP9844720.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4438..5526
                     /gene="pdeL"
                     /locus_tag="ACHOTW_002638"
     CDS             4438..5526
                     /gene="pdeL"
                     /locus_tag="ACHOTW_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9844721.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5568..6499)
                     /gene="yahB"
                     /locus_tag="ACHOTW_002639"
                     /pseudo
     CDS             complement(5568..6499)
                     /gene="yahB"
                     /locus_tag="ACHOTW_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6591..7098)
                     /gene="yahC"
                     /locus_tag="ACHOTW_002640"
                     /pseudo
     CDS             complement(6591..7098)
                     /gene="yahC"
                     /locus_tag="ACHOTW_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7356..7961
                     /gene="yahD"
                     /locus_tag="ACHOTW_002641"
     CDS             7356..7961
                     /gene="yahD"
                     /locus_tag="ACHOTW_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP9844722.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            8001..8864
                     /gene="yahE"
                     /locus_tag="ACHOTW_002642"
     CDS             8001..8864
                     /gene="yahE"
                     /locus_tag="ACHOTW_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9844723.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8854..10401
                     /gene="fdrA"
                     /locus_tag="ACHOTW_002643"
     CDS             8854..10401
                     /gene="fdrA"
                     /locus_tag="ACHOTW_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9844724.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10401..11819
                     /gene="yahG"
                     /locus_tag="ACHOTW_002644"
     CDS             10401..11819
                     /gene="yahG"
                     /locus_tag="ACHOTW_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9844725.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            11845..12165
                     /gene="yahH"
                     /locus_tag="ACHOTW_002645"
     CDS             11845..12165
                     /gene="yahH"
                     /locus_tag="ACHOTW_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
                     /protein_id="HFP9844726.1"
                     /translation="MNALSGLRMAQESVGLISVAHQAFVTIAGCGVNALSGLQVAQEF
                     VGLISVAHQAFITIAGCGVNALSGLRMAQESVGLISVAHQAFVTIAGCGVNALSGLRM
                     AREL"
     gene            12192..12284
                     /gene="yahH"
                     /locus_tag="ACHOTW_002646"
                     /pseudo
     CDS             12192..12284
                     /gene="yahH"
                     /locus_tag="ACHOTW_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
     gene            12334..13284
                     /gene="yahI"
                     /locus_tag="ACHOTW_002647"
     CDS             12334..13284
                     /gene="yahI"
                     /locus_tag="ACHOTW_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP9844727.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            13294..14676
                     /gene="yahJ"
                     /locus_tag="ACHOTW_002648"
     CDS             13294..14676
                     /gene="yahJ"
                     /locus_tag="ACHOTW_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9844728.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            14975..15385
                     /locus_tag="ACHOTW_002649"
     CDS             14975..15385
                     /locus_tag="ACHOTW_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9844729.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            15639..16622
                     /locus_tag="ACHOTW_002650"
     CDS             15639..16622
                     /locus_tag="ACHOTW_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9844730.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            16671..18155
                     /locus_tag="ACHOTW_002651"
                     /pseudo
     CDS             16671..18155
                     /locus_tag="ACHOTW_002651"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            18148..19119
                     /locus_tag="ACHOTW_002652"
     CDS             18148..19119
                     /locus_tag="ACHOTW_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9844731.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            19116..20072
                     /locus_tag="ACHOTW_002653"
     CDS             19116..20072
                     /locus_tag="ACHOTW_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9844732.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            20159..21208
                     /gene="yahK"
                     /locus_tag="ACHOTW_002654"
     CDS             20159..21208
                     /gene="yahK"
                     /locus_tag="ACHOTW_002654"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP9844733.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            21451..22266
                     /gene="yahL"
                     /locus_tag="ACHOTW_002655"
     CDS             21451..22266
                     /gene="yahL"
                     /locus_tag="ACHOTW_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFP9844734.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            22718..22927
                     /locus_tag="ACHOTW_002656"
     CDS             22718..22927
                     /locus_tag="ACHOTW_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844735.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(22944..23615)
                     /gene="yahN"
                     /locus_tag="ACHOTW_002657"
     CDS             complement(22944..23615)
                     /gene="yahN"
                     /locus_tag="ACHOTW_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP9844736.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            23762..24037
                     /gene="yahO"
                     /locus_tag="ACHOTW_002658"
     CDS             23762..24037
                     /gene="yahO"
                     /locus_tag="ACHOTW_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP9844737.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(24135..25721)
                     /gene="prpR"
                     /locus_tag="ACHOTW_002659"
     CDS             complement(24135..25721)
                     /gene="prpR"
                     /locus_tag="ACHOTW_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP9844738.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
BASE COUNT         6633 a         6014 c         6886 g         6315 t
ORIGIN      
        1 gtatattaaa tgtatttctc ctgcttttgt ttatattttg agttgatttg agttcccgga
       61 tacatataat gcgcaggtat tagagactgg ggggaaatgt aatgataaag ttaagagtgt
      121 taacttaaaa taagttttta gtagtaactt gctattttat taccctctga actacatact
      181 cagatatgtg gttgccaatg ctatgcaaaa tttttgaatt tttatatgcg atgaacagta
      241 accggaatgg catttaatgc gttgctgttt atgcaatctt ggttttattt ggatgtattg
      301 ctggagattg tgatgaataa aatatatcgg ctaaagtgga acaggtcccg taactgttgg
      361 agcgtctgct cggagctggg gagcagagta aaaggaaaaa agtcccgggc tgttttaatt
      421 agcgcgataa gtttatattc atctctggta ttcgccgatg atgtcatcgt aaaccaggat
      481 aaaactattg attttggcaa agagaaccag agcatcgatt accgtattac ggtgacagac
      541 aatgccaatc tggtaatcaa tgcgacagat acttcccgtc cgcgtctgac tctcgcttct
      601 ggtggtgggt tggatattac cggaggaaag gtaacaatca atggcccact taactttttg
      661 ctgaaaggta cggggttcct gaatgtctcc aatgctggca gcgagttata tgctgatgat
      721 ttgtatgaat caaactcagg catgagacac gatcgcggct attttaatgt ctccaacggc
      781 ggcaaaatcc atgttaaggg caccagccgt ctgacctatt tgcagggaaa tgtcagtggt
      841 gaaggtagcc aggtaaattc cgaaaccttc tttatgggcg tttacggcag ttacggtggt
      901 aatcagtacc tgtcagttaa taacggcggt gaagttaatg ccaggaagca aattagcctg
      961 ggctattatg atcaagtctc cgatacaaca cttgctgttt cggaaggtgg taaaatttct
     1021 gcgcctacta ttagtttaag caccaactct gagttagcgt taggggcaca ggaaggaagc
     1081 gcagcgaagg cagcagggat tattgatgcc gaaaaaattg agtttgtgtg ggcaaagaca
     1141 tccgagaaga aaatcacctt aaaccacacg gataaagacg cgactatttc cgcggatatt
     1201 gtcagtggca gcgagggcct gggctatatc aatgcgctca atggcacgac ttacttaacc
     1261 ggtgataact ctgcctttag tggtaaagtc aaaattgagc aaaatggcgc tttagggatc
     1321 acccaaaata taggtacagc agagatcaac aaccgcggga aattacacct gaaggctgac
     1381 gatagcatga cctttgccaa taagatctct ggcaacggta caataagtat cgacagtggg
     1441 acggtggagt tgaccggcaa taactatgca ttcagcggat atattgatgt tgcttctggt
     1501 gctgtcgctg ttatttctga agacaagaat atcggtcgtg cagagctgga tgtcgatggc
     1561 aaattgcaaa ttaatgccaa caaagattgg gtatttgata acgatcttga aggtagaggc
     1621 attgttgaaa taaacatggg gaatcacgaa ttctccttcg atgagtttgc ttatacagac
     1681 tggttccagg gttcactggc gttccagaac acgacattta atctggaaaa gaatgctgag
     1741 tttctgcaga aaggcgggat cactgcgggt cagggaagcc tggtaacagt gggtaagggc
     1801 gctcactcca ttagcacttt gggattctcc ggcggaaccg ttgattttgg tgccctgaca
     1861 gcaggtgcac agatgacaga agggacggtc aacgttagta aaacgctgga tttgcgcggc
     1921 gagggtgtga ttcaggtttc tgacagtgac gttgtccgct cagtatctcg tgatattgac
     1981 tctgcgttat cgctcactga agtcgatgat ggtaacagca ccattaagtt ggttgatgcg
     2041 caaggtgcgg aagttctggg cgatgcgggc aatctgcaat tgcaggataa aaatgggcaa
     2101 atcctctcca gcagcgccca acgtgatatt cagcagaatg ggcaaaaagc ggccgtcggc
     2161 acttacgact atcgtctgac gagtggggta aacaatgacg gtctgtatat tggttacggc
     2221 ctgacccagc ttgatttaca cgctaccgac agcgatgctc tggtgctgag ctctaacggt
     2281 aaaagcgaga atgccgccga tctcagcgca aagattaccg gcagtggtga cctggcattc
     2341 agcagccaga agggtcagac cgtatcgctt tctaacaaag acaacgacta taccggtgtt
     2401 accgatctgc gcagtgggac gcttttgttg aataacgata acgtgttggg taatacccat
     2461 gaactgcgtc tggcggcaga gactgaactg gacatgaatg gtcacagcca gaccgtgggc
     2521 acgctcaatg gcagcgccga ttcactgctg agcttaaatg gcggcagtct gacggttacc
     2581 aacgggggca cttcaaccgg ttcgttaacg gggagcggag agctgaatat tcagggcggc
     2641 acgctggaca tcgcgggcga taacagcaac ctgacggcga atgtgaacat tgctaattcg
     2701 gctaatgtcc tggtaagtca tgcgcaggga ttgggtagcg caaacgttga gaacaacggt
     2761 accctggcgt tgaataatag cgctgaaaaa agagcggctg cgtctgtgaa ttacgccctg
     2821 ggcggcaatc tgaccaacaa cggtacgctg atgaccggaa tgtcaggaca gcaagctggc
     2881 aatgtgttag tggtgaaggg gaactaccac ggtaataacg gtcaactagt aatgaatacg
     2941 gtactgaatg gcgatgactc agtaaccgat aaattggttg tcgagggcga tactagcggc
     3001 acgactgccg ttacggtgaa taacgctggc ggtacaggtg cgaaaaccct taacggtatc
     3061 gaacttatcc atgtagacgg taagtctgag ggcgaatttg ttcaggctgg gcgtatcgtt
     3121 gcgggggcgt atgactacac tctcgcgcgt ggacaagggg caaatagtgg taactggtat
     3181 ctgaccagcg gcagtgattc tcctgaactg cagccggagc cagacccgat gccgaatcca
     3241 gagccaaacc cgaatccaga gccgaaccct aacccgacac ctacgccggg tccggatctg
     3301 aatgtggata atgacctgcg accggaggcg ggtagctaca ttgcgaacct tgcagcagcg
     3361 aataccatgt tcaccacgcg tctgcatgag cgtctgggta atacgtacta taccgacatg
     3421 gtgacgggtg agcagaaaca aaccactatg tggatgcgcc atgaaggtgg tcataataaa
     3481 tggcgtgatg gcagcggcca gctgaaaacc caaagcaatc gctatgttct gcaactggga
     3541 ggcgatgtcg cgcagtggag ccaaaacggc agcgaccgct ggcatgttgg ggtcatggcg
     3601 ggatatggca acagcgacag caaaaccatt tcctcgcgaa ccggttatcg tgcaaaagcg
     3661 agtgtgaacg gatatagcac aggcctctat gccacctggt atgccgatga cgagtcgcgt
     3721 aatggcgcgt atctcgacag ttgggcgcag tacagctggt ttgataacac agtgaaaggg
     3781 gatgacttac aaagtgaatc ctataaatca aaaggattta ccgcttcact ggaagctgga
     3841 tacaaacaca aattagctga atttaatggc agccagggaa cgcgtaatga atggtatgtt
     3901 cagccgcaag cacaggttac ctggatggga gtcaaagccg ataagcaccg cgaaagcaac
     3961 ggaaccctcg ttcatagcaa cggtgatggc aatgttcaaa cccgacttgg cgtaaaaacc
     4021 tggctgaaga gccaccataa aatggatgac ggtaaatccc gcgagttcca gccgtttgta
     4081 gaagtgaact ggctacataa cagtaaggat ttcagcacca gtatggatgg cgtgtctgtc
     4141 actcaggatg gagcccgaaa tattgctgag ataaaaaccg gggtggaagg acagctaaat
     4201 gccaacctga atgtctgggg gaatgtgggc gttcaggttg ccgatagggg atataatgac
     4261 acctctgcaa tggttggcat taagtggcaa ttctgatctg aataatgagt aaaaggagag
     4321 agagccgaac gggagataat tcggctctct gtgtcatttt cttgcagcca gctttatccg
     4381 tcagagtgaa gactcgcaga tttagctggc gcacaagatt taatcaaggt gtgttcaatg
     4441 gcttcgcatg atttgagtgt ttttttggaa gagttcgggg caacggtgaa tttgacgttg
     4501 cctggtatcg tgtccgaaaa agaacggctt ttactcaagc tgctaatgga gggaatgtcg
     4561 gttacagaaa tctcacagta cagaaatcgc agtgcaaaga caatttcaca tcaaaagaag
     4621 cagctgtatg aaaaactagg catccagagt gatattacct tctggcggga tatctttttt
     4681 caatatcatc cgcaagtcat tagtggcacg ggaaataaaa ataattttta tatccctgat
     4741 aaccgttgtc accatatcgt cacgcctgaa gccatcagtc tggcgttgga aaaccatgaa
     4801 tttaaaccgt ggatccaacc ggttttctgc gcgcagactg gggtactgac gggctgtgaa
     4861 gtgcttgtcc gctgggaaca tccacaaacg ggaatcatcc cgccggatca gtttattcct
     4921 ctggcggagt catccggtct tattgtcata atgacccgcc aactgatgaa acagactgcg
     4981 gatattctga tgccggtaaa acatttgctg ccggataatt tccatattgg tatcaacgtc
     5041 tcggcgggtt gttttttggc cgcgggattt gaaaaagaat gcctgaacct ggtcaagaaa
     5101 ttaggaaacg ataaaatcaa actggttctc gagctaacgg aacgtaaccc tattccggta
     5161 acgccagaag ccagagcgat atttgacagc cttcatcagc ataacattac ctttgcgctg
     5221 gatgactttg gtacgggtta tgcgacctat cgttatctgc aggcgtttcc ggtcgatttt
     5281 attaagatcg ataagtcatt tgtgcaaatg gcgggtgtcg acgaaatatc cggtcatatt
     5341 gtggacaata ttgtcgaact ggcgcgtaag cctggtctga gtatcgtggc ggaaggggta
     5401 gaaacccagg agcaggcgga tttaatgatc ggtaaaggcg ttcacttttt gcagggctat
     5461 ttgtactctc cgccagtacc gggtaataaa tttatctctg aatgggtaat gaaagcaggt
     5521 ggttgatgta aaccgctatt cacagcgcat cgggaggttg gcagcgatta attctcccga
     5581 tgcagtaaat cctgatagat ccccgttaat atgccatttg gcgcaaattg ttttttaatc
     5641 cactgcgtca cctgacccgt agcagaatgc tgagtcgcca gtaacatgcg tgaatcctgg
     5701 cgcgggttat ggatttgtcg ggtaaccagc agggattgcg ccatcgcctc acggaccatg
     5761 taatctggca aaaaaacaat cccttcacct aaaatctggc actgacattt ggtgttgaaa
     5821 tctggcacca gaattgactc ctgcccgtgc agcaaccagc ccactttttt attaatcgta
     5881 tgcgcggtgt cctccaccat aatattaggg tagagacgca gttggctttc ggcgatggtt
     5941 ccggcacaaa ggctaacggg tgatccgggg cgatagcaaa agcccagcga atcgcgccaa
     6001 tttcagtgta atcaataccg ccgccgtcca gcagcgtgtc gggtgcgccg atagcgatat
     6061 ttgcctgatt attaataatc gcatcccaga cgccgttata cacttcggtg gtgacggtaa
     6121 tctggcaggt aggaaactgc tttttcagca cctgtaataa gcgtgcggtg tgtttgggcg
     6181 tataaagcaa ctgattaata caaatgcgca cacgcgcttc tatgccctgc gaaatagtat
     6241 cgatgctgcg tttgatggcg tgaaaatcat tcagcaggtc ggtagctttg cggaaaaaat
     6301 aacgcccgga ctccgttaac tcaatgctgc gggtactgcg agtgaacagc accacatcca
     6361 gccctgtttc cattcgcttg atggtgtagc taatggcgga agtggttaaa cccaactctt
     6421 ctgcggcttt actgaaactg ccaaaacgcg cagcggtagt aaatgccaac agattctctt
     6481 cggtaaaaat tgagttcatt aaaccattcc cgccattatc taatatgaat ttctccaagc
     6541 ataactgtag aggtatcaat tagagatggg taatcatttt tgaataactt ttaatacccg
     6601 tcgcgtttag tcgtcgcttc gcttaatttt tgtccgccat attctgaaat tatccccacg
     6661 cagttacccg ccagaaaacc cagtaacgtc accgtccagt tcatcccgct ggcaaaaaac
     6721 agcgtcatgc cgagaaagcc accgggaata aatgacagta accagaaacg cccttgccag
     6781 accaccaccg ccgagaaggg tacggtagta attacgctcg cccagaacgc ggacagtcca
     6841 cttacagagg ccagccaacc ggcagccagc gcggcgaaaa acgcccacac catcccggaa
     6901 taattaaccg acagacttct gataaacccc gatttaccgc cgcctgcggc ataaaaactg
     6961 caaaaggcga cgaagccaat ggttcctaat aattcctaat aattcccatc cctgagagag
     7021 cccgacatgt gaagacacta actgccaaag ccctgcacaa ataccgacgg tgacacccgt
     7081 tgccgtgagt ccgttcatct tgttcccctc tggttagaca tggacgaaag ctactcttac
     7141 atgtgcagac tatagagaaa taaaacctga ttgagtgaaa gggtatcggg tcaaagaaac
     7201 aaatattgca ctaccgcaca ctgcgaaaag attgttgaac atcattcaac aaaaaggcga
     7261 aatacggcga attaaattta agcgggttct tttatcagat tatcgttgct attctcaatt
     7321 catcgaaaac agccacaaaa accaggggaa tgtgaatgag tataaaaaat ctacctgccg
     7381 actatttatt ggctgcacaa cagggtgata tcgataaagt aaaaacctgc cttgcgctgg
     7441 gtgtcgatat taatacctgc gatcgtcagg ggaaaacggc aattacgctg gcaagtttat
     7501 atcagcaata tgcttgcgtt caggcattaa ttgatgccgg agcggatatt aataagcaag
     7561 atcatacctg tttaaatcct tttttaatta gctgtctgaa cggtgattta acgctgctac
     7621 gaattatttt accggctaaa ccagacctta attgcgtcac ccgttttggc ggtgtcggcc
     7681 tgacgcctgc ctgtgaaaaa ggccatttaa gtattgtaaa agagcttttg gcgcatacgg
     7741 aaattaacgt taaccagacc aaccatgttg gctggacgcc actactggaa gcgattgtgc
     7801 ttaacgatgg cgggattaaa cagcaggcga ttgtgcagtt attgctggaa cacggtgcca
     7861 gcccgcatct gaccgataaa tatggcaaaa cgccactgga actggcgcgg gaacggggct
     7921 ttgaagagat tgcgcagtta ctgattgccg caggtgcata aaccgggagg cttgctatca
     7981 acacaccaga aaaacggtgt gtgtgggcgc taactgcgga tgcggatttt ctggcgcagc
     8041 gagggcaagg acaggttgaa caggtctttg ccagagcggt aaatatcgca ctcccggctc
     8101 gccagcagtt gctgacgctg ctttgtgaag agtacgacaa tgcgccaaac agttgtcggt
     8161 tggcactcac tcactttgat gatctgttcc ggcatggtga taaggttcag tttgacgatc
     8221 aaggtattac ggttggtcaa catcttcata tagagatgag tcgttgtcgg cgttggctgt
     8281 ccccaacctt gcaaatgacc gctgtgaatt ttcgccttat cgcctggcaa cagtggcacg
     8341 acatcattca tcagcacctg ggggaaaatg aaaccctgtt taattatcgc ggcgataatc
     8401 cgttttatca ggcgttaaat aaagaattac atattaaacg acgggcagtt atacaggcag
     8461 taatcgataa acaaaatatc gcctcagcgg tcgccagtat gatgggctta gggattggcc
     8521 ttacgccatc agccgatgat tatttaacag gtctggcact tattttattt atttccgggc
     8581 atccggcggg aaaatacaaa gaggaatttt atctcggctt gcaacgcggc agaaataata
     8641 ccacgttatt aagtgccata acgctggaag ccgcattaca acaacgctgc cgggaaaata
     8701 ttcatcgttt tattcacaac attatttatg acatccctgg gaacgcaact caggcaatag
     8761 aaaaaattaa acatattggc tccagttccg gctgcgacat gctgtatggc atggccgatg
     8821 gttgtgcgct gagccaaacc tacggaggga attatgtcag ttaaaatagt cattaaaccg
     8881 aatacctatt ttgattctgt ctcgctgatg tctatctcca cgcgtgcaaa taaactcgac
     8941 ggcgtcgagc aggcatttgt ggcgatggcg accgaaatga acaaaggtgt gctgaaaaat
     9001 ttaggactgc tgacgccgga gctggagcag gcgaaaaacg gcgacctgat gattgtcatc
     9061 aatggtaaat cgggggcgga caacgagcag ttgctggtgg agattgaaga actgttcaac
     9121 accaaagcgc aaagcggctc gcacgaggcg cgttacgcca ctattgccag cgccaaaaag
     9181 catatcccgg aaagtaacct ggcggtgatt tcggtcaacg gtctgtttgc cgctcgcgaa
     9241 gcgcgtcagg cgctgcaaaa cgatctcaac gtgatgctgt tttccgataa cgtctcagtt
     9301 gaagatgaac tggcgctcaa gcaactggcc cacgaaaaag ggctgctgat gatggggcca
     9361 gactgcggca cggcgattat caacggtgcg gcgctctgct ttggtaacgc cgtgcgtcgc
     9421 ggcaacatcg gtattgttgg cgcatccggc accggcagtc aggagttgag cgtccgcatt
     9481 catgaatttg gcggcggcgt ttcgcaactg attggcaccg gcgggcgcga cctgagcgag
     9541 aaaatcggcg gcctgatgat gctcgacgcc atcgggatgc tggaaaacga tccgcaaact
     9601 gaaatcattg cgcttatctc caaaccgcct gcgcctgcgg tggcccgcaa agtgctggaa
     9661 cgtgcgcgcg cctgccgcaa gccggtggtc gtttgcttcc tcggtcgtgg cgaaacgcca
     9721 gtggatgagc aggggctaca gtttgcccgc ggcaccaaag aggcagcgct aaaagcggtg
     9781 atgctctccg gcgtgaaaca agaacatctc gacctgcata ccctgaacca gccgttgatt
     9841 actgacgtgc gcgctcgcct gaagccgcag cagaaataca ttcgtggcct gttctgcggt
     9901 ggcacgctgt gcgatgaaac gatgttcgcg gtgatggaaa aacacagcga tgtctacagc
     9961 aacatccagc ccgatccgga attccgcctg aaggatatca accgcagcat caaacacacc
    10021 ttcctcgact ttggcgatga cgacttcacc aacggcaagc cgcacccgat gattgaccct
    10081 accaaccgca tcagccgctt gatcgaagag gcgcgcgatc cagaagtggc ggtgatcgtg
    10141 atggattttg tgctcggatt tggatcgcat gaagatccgg tcggttccac catcgaggcg
    10201 atcaaagaag cgaaagcgat cgccgctgcc gaaggacgcg agttgatcat tctcgcctat
    10261 gtgctgggta ccgatcttga tacgccatcg ttagaacaac aaagccagat gctgcttgat
    10321 gccggcgtga ttctggcgag cagcagcacc aataccggat tgctggcgcg tgaatttatc
    10381 tgcaaagggg aggaagcctg atgagccagt cactgtttag ccaaccattg aacgttatta
    10441 acgtcggcat cgccatgttt agcgatgacc tgaaaaagca gcatgtagaa gtgactcaat
    10501 tcgactggac gccgccgggg cagggcaata tgcaggtggt gcaggcgctg gataacattg
    10561 ccgattcgcc gctggcagac aaaatcgccg ccgctaacca gcaggcgctg gagcgcatta
    10621 tccagtcgca tccggtgctg attggttttg atcaggcgat taacgtggtg ccgggcatga
    10681 cgccgaaaac cattcttcac gccgggccgc cgatcacctg ggaaaaaatg tgcggcgcga
    10741 tgaaaggcgc ggtcaccgga gcgctggtgt tcgaaggact ggcgaaagat ctcgacgagg
    10801 cggctgaact ggcggcttcc ggggagatca ccttctcgcc gtgtcacgag cacgactgcg
    10861 tgggatcgat ggcgggtgtg acctcggcct cgatgtttat gcacatcgta aaaaacaaaa
    10921 cctacggcaa catcgcttat accaacatga gcgagcagat ggcgaagatt ttgcgtatgg
    10981 gcgctaacga ccagagcgtg attgaccgcc tgaactggat gcgtgatgtg cagggaccaa
    11041 tactgcgcga cgcgatgaaa attatcggcg aaatcgatct gcgcttaatg ctggcgcagg
    11101 cgctgcatat gggcgatgag tgccataacc gcaataacgc cgggacgacg ctgctgattc
    11161 aggcgctgac gccggggatt atccaggcgg gctattccgt cgcgcaacag cgcgaagtgt
    11221 ttgagtttgt tgccagtagc gattacttct ccggcccgac gtggatggcg atgtgtaaag
    11281 cggcgatgga tgcggcgcac ggcatcgaat acagcaccgt cgttaccacc atggcgcgta
    11341 acggcgtcga gttcggcctg cgggtgagcg gcctgccggg gcagtggttt accggcccag
    11401 cgcagcaggt catcggcccg atgtttgctg gttataagcc ggaagattcg gggctggata
    11461 tcggcgacag cgccatcaca gaaacctacg gtattggcgg gtttgccatg gcgaccgcgc
    11521 ctgcaatcgt cgcgctggtg gggggcacgg tggaagaggc tatcgatttc tcccgtcaga
    11581 tgcgcgaaat cacccttggc gaaaacccca acgtcaccat tccgctgctc ggttttatgg
    11641 gcgtgccgtc ggcagtcgac atcacccgcg tgggtagcag cggcattctg ccggtgatca
    11701 acaccgccat cgcccataaa gatgcgggcg tcggcatgat tggcgcgggc attgtgcatc
    11761 caccattcgc ctgcttcgag aaagccattc ttggctggtg cgaacgttac ggcgtttgac
    11821 ctacctgttc ataccggatg cggcgtgaac gccttatccg gcctacgaat ggcgcaagaa
    11881 tctgtaggcc tgataagcgt agcgcatcag gcatttgtca ccattgccgg atgcggcgtg
    11941 aacgccttat ccggcctaca agtggcgcaa gaatttgtag gcctgataag cgtagcgcat
    12001 caggcattta tcaccattgc cggatgcggc gtgaacgcct tatccggcct gcgaatggcg
    12061 caagaatctg taggcctgat aagcgtagcg catcaggcat ttgtcaccat tgccggatgc
    12121 ggcgtgaacg ccttatccgg cctacgaatg gcacgagaat tgtaggcctg ataagcgtag
    12181 cgcatcaggc atttgtcacc attgccggat gcggcgtgaa cgccttatcc ggcctacgaa
    12241 tggcacgaga atttgtaggc ctgataagcg tagcgcatca ggcagtctgg cgttggtcat
    12301 aaccccatca ccctctgtag cggacataac aacatgaaag aacttgtggt cgttgccatt
    12361 ggtggcaaca gcattatcaa agataacgcc agccagtcga ttgagcatca ggcggaggcg
    12421 gtgaaagccg tggccgatac ggtgctggaa atgctggctt ccgattacga cattgtgctg
    12481 acccacggta acgggccgca ggtcgggctg gatttacgcc gtgcggagat tgcccacgag
    12541 cgcgaagggc tgcccttaac gccgctggcg aactgtgtgg cagatacgca gggcggcatt
    12601 ggctacctga tccaacaggc gctgaacaac cggctggcgc gtcacggcga gaaaaaagcc
    12661 gtcaccgtgg tgactcaggt ggaagtggat aaaaacgatc caggttttgc ccatcccacc
    12721 aagcccattg gtgcattctt cagtgaaagc cagcgtgacg aattacaaaa ggcaaaccct
    12781 gactggcgtt ttgttgaaga tgctggacgg ggctatcgcc gcgtggtcgc ctcgccggaa
    12841 ccgaaacgta ttgtcgaagc acccgccatt aaggcgctga tccaacaagg ctttgtggtg
    12901 attggcgcgg gcggcggcgg aattcctgtg gtgcgtactg acgcgggaga ttaccaaagc
    12961 gtggacgcgg ttatcgacaa agatctctct accgcgctgc tggcccgtga aattcacgcc
    13021 gacattctgg tgatcaccac cggcgtcgaa aaagtctgta tccactttgg taaacccgag
    13081 aagcaggcgc tggatcgggt ggatattgcc accatgactc gctatatgca ggaaggacat
    13141 ttcccgcccg gcagcatgtt gccaaaaatc atcgccagcc tgacgttcct ggaacagggc
    13201 ggtaaagaag tgattatcac cacgccggaa tgcctgccag cggcgctgcg cggtgaaacg
    13261 ggcacccaca ttattaaaac gtaaggacgt aagatgaaag aaaataatag ccgccgtgaa
    13321 tttctgagcc agagcggtaa gatggtcacc gccgccgcgc tgtttggtac ctctgtgccg
    13381 ctcgcccatg cggcggtctc tggcaccaca aactgcgaag cgaacaacac catgaaaatc
    13441 actgacccgc attactatct cgacaacgtg ctgctggaaa ccggttttga ctacgaaaat
    13501 ggcgtggcgg tgcagacccg cacggcgcgc cagaccgtgg agattcagaa cggcaaaatt
    13561 gtcgcgctgc gcgagaataa gcagcacccg gatgccacgc tgccgcacta tgacgctggc
    13621 gataagctga tgctgcccac cacccgcgac atgcatattc atctcgacaa aacgttttac
    13681 ggcgggccgt ggcgctcgct caatcgcccg gcaggcacca ccatccagga catgatcaaa
    13741 ctcgagcaga aaatgctgcc ggagctgcaa ccgtacactc aggagcgggc agaaaaactg
    13801 attgatttat tgcagtcgaa aggcaccacc attgcccgca gccactgcaa tatcgaaccg
    13861 gtttccggcc tgaaaaatct gcaaaatttg caggcggtgc tggcgcgacg tcaggcgggc
    13921 tttgagtgtg aaatcgtcgc cttcccgcag cacggtttgc tgctgtcgaa atcggaaccc
    13981 ttaatgcgtg aagcaatgca ggcgggtgcg cattacgtcg gcgggctgga cccgaccagt
    14041 gtcgatggcg cgatggaaaa atccctcgac accatgttcc agattgcgct ggactacgac
    14101 aaaggcgtcg atattcacct gcacgaaacc actccgtcgg gcgtggcagc catcaattat
    14161 atggttgaaa cggtagagaa aacgcctcaa ctgaaaggta agctgaccat cagccacgcc
    14221 tttgcgctgg ctacgctcaa cgagcaacag gtagatgaac tggcgcatcg catggcggcg
    14281 cagcaaattt ctatcgcctc gacggtgccg attggcacgc tgcatatgcc gctcaaacag
    14341 ttgcacgaca aaggcgtaaa agtgatgact ggcactgaca gcgttatcga ccactggtcg
    14401 ccttatggtc tgggcgacat gctggaaaaa gccaatctct acgcgcagct ctatattcgt
    14461 cctaacgaac agaacctctc ccgttcgctg tttctagcca ctggcgatgt attgccgctg
    14521 aatgaaaaag gcgagcgtgt atggccaaaa gcgcaggatg acgccagctt tgtgctggtg
    14581 gacgcctcct gttccgccga ggcggtggcg cgtatctcgc cgagaaccgc aacgttccat
    14641 aaagggcaac tggtgtgggg gagtgtggca ggttgatgcg catttgaaac gcctgcaacg
    14701 gtgaatagta agagatttaa gccccaggga gtggggcggg aatggtttgc taatgaatga
    14761 tcctgaactt tataatctcc cggcataatt tccaggggat tatttgagag aaatacacaa
    14821 aagtgagttt taacaaaggt atttatcagg ccataaatgt gacctgcttc ggataaaaaa
    14881 gattcagtat cgaaaaacca atgatactct tgctgatggt taccaacagt aattaagatg
    14941 tgatttgttt ttccgtaatc gcaaaaatat tgccatgacc ataaaagaaa aattctcaca
    15001 aaaatatccc catgcttcct tttgtacttt tggcgattca gccgcgttgg ccgatcacct
    15061 cgcaacgttg attgcgactg gtgttaaaac agcttcctgt ggttcgctgg cgggatgtat
    15121 tgaagacaac gcatttccga tgattggtga gtataagatt gtagaaaata gccgtggtga
    15181 gcctgtatgt gttatcagag tgattggcct gcatttactg cgcttttctg atgtcaccgc
    15241 ggagcttgcc cgtaaggaag gtgaaggtga cctcagtctt gaatattggc gtaatgaaca
    15301 ccgtcgtttt ttccaggcag aaggaagcta ttcaccagaa atggacgtta tctttgaaga
    15361 atatgccctt attgacgttg tgtagataac tatttatcta catgaaataa gctcacaaac
    15421 ataaaatatc gctaattttg tttattttgc tgttttttac gtgatgaatg tcacacttat
    15481 tattgctgcc cgttttgtcc atatttataa ccatattttt aatagctgcc tgaaatagag
    15541 ctgtttattc tgcataaaaa tattcgaaaa ttcttattct ctccagcgcg ctaatttggg
    15601 cgaacacttc ctgactaccc tgcaatgagg ctgaaatgat gaataaacgt tttgttatca
    15661 atatggtgtc ttcgctgtta ttaggcgctg cgctaatttc tgcgccgctc caggcagcgg
    15721 aaaaagtcgt ggtgaatatt tctaaagtcg atggtatgcc gtggtttaat cgcatgggcg
    15781 aaggtgtggt tgaggcgggt aaagcctttg gtgttaatgc ctcacaggtg gggccttcaa
    15841 gtactgatgc tccgcaacaa gttaaaatta ttgaggattt aatcgcccgt aaggttaatg
    15901 caattactat cgtgccaaac gatgccaacg tgctggaacc ggtattcaaa aaagcgcgag
    15961 atgccggaat tgtggttctg accaacgaat cccccggtca gccaagtgct aactgggata
    16021 tcgaaatcat tgataacgaa aaatttgccg ctgaatatgt tgagcatatg gcgaaacgca
    16081 tgggtggcaa gggcggctat gtgatttacg tgggtagttt gaccgtgccg cagcataact
    16141 tatgggctga cttgctggtg aaataccaga aagagcatta cccggatatg catgaagtta
    16201 cccgccgcat gccggttgcc gagagtgtgg atgtctcacg tcgtactaca ctggacctga
    16261 tgaaaaccta tcctgacttg aaagcggttg tctcctttgg ttcaaacggt ccgattggcg
    16321 cgggacgtgc ggtgaaagag aaacgggcga aaaataaagt cgcagtctat ggcatgatga
    16381 tcccttcaca ggcggcatca ctgattaaaa gtggtgatat caccgaaggg attacttacg
    16441 atccggcctc tgcaggttac gcactcgcag ccgtcgccag cacgttgctg aagggtgaag
    16501 agatcaagcc tggattagaa atgcagaatc tgggtaaggc cgacgtagat atggataagc
    16561 gaattatccg tttccataag gtattactgg tcaacaaaga caatatcgac tcactttatt
    16621 aatccatttc ttcggggcga aagccccgat tcttattgcc aggggatgtt atggaaacct
    16681 tcctttccct tcgtcatatc aacaaaacgt ttcatgccac gcgagcgctg cgcgatgttt
    16741 ctctcgactt tatgtcgggt gaagtgcact gccttgccgg gcaaaacggc tgtggtaaat
    16801 cgacgttgat taaaattatg tccggtgttt atcgcccgga tgagggagcg gaaattacgc
    16861 ttggtgggaa aaactggtca aagctgacac ccgccgcttc ggtggcgcag gggattcagg
    16921 tgatttatta ggacctctct ttatttccta acctgagtgt ctgggagaat atcgccgtga
    16981 atcactatca ccacggtctg tttgttaacc gccgtcgtct gcgtgaggtg gcgcaggcgg
    17041 caatgactag tattaacgtc acattgccac tggatacgct cgtttctgag ctttcaattg
    17101 cccgctgtca gctggtggca atttgccgtg cactggcgca ggacgcacgg ttgatcgtga
    17161 tggatgaacc aaccgcttcg ctgacccatc aggaagtgca agggttatta caggtggtgc
    17221 atcaattgcg tgaacgcggg atctgcgtgg tctttgtcag tcatcgtctg gaagaagtga
    17281 tggaagtttc cgaccgtatt tcagtgctga aagatggtga gctggtcggg acttttccgg
    17341 ctgcagagat gaccacaaaa caactcggtt tcctgatgac cggccaggag tttgaatatc
    17401 aggtgcgaga gttgtggcag ggaaaatcca gcaccccggt gctggaggtg cgtaatttaa
    17461 gtcgtcatgg ggaatatcta aatattaact tgcgggtgga agcgggcgaa gtggtatcga
    17521 ttgttggcct gcttggtgcg gggcgcacgg aattatgtct gagtctgttt ggtatgaccc
    17581 gaccagatgc tggcgagatc ctcatcaacg gccagctggt tacactgcat agtaaccagg
    17641 acgccatccg ccacggtatt ggttatgttt cggaagatcg catgtcgcgc ggtttggtga
    17701 tggcgcaatc tattgaagac aacatcatta gcactgtttt tcacaaggta aaagatcgct
    17761 tcggctttct gagtgaaact aaagtgcgtg acctggtgga caggctaatc aaggcattga
    17821 ccatcaaagt ctctgatcct catcttccgg tgaatacgct ttctggtggt aacgcgcagc
    17881 gagtatcgat cgccaaatgg ctggcgattg ggccacgact gttgattctc gatagcccga
    17941 cagtcggtgt tgatatcgcc aacaaagcag gaatatacgg cattatcagc gatctggcgg
    18001 ctcatggcat tgcggtattg atgatctgcg atgaaattga agaagcgtgg tatcaaagcc
    18061 accggatttt ggtaatgcaa aaaggtcaga tcacccatag cttcctgcct gacagcagtt
    18121 ctcaggccag gattgcggag gtggtaaatg gctgaactga aaaaacgcca tgaattctgg
    18181 ctggcattac tgattgtggt gctgtttgtt ggccttgcct ggcgcagcga cgagtttctg
    18241 acattcggta atttgtacga tctcgccaat aactatgcca tgttgactat tctcgcctgt
    18301 ggtttgtttg tggtgttgat ttccggtgga attgatattt cgtttccagc aatgaccatc
    18361 attgcgcaat acggcatggt gctgttgctg caaaaaattg gtggcaactt cgctgtcgcg
    18421 tttgcactgg caggctgcat cggcatttta cttggcttaa ttaacgcctt actggttaat
    18481 cgcctacggg tgccttctat catcatcact atctcgacgc tgaatatttt ctatggcctg
    18541 ctgttatggt tgagtaaagg tgtgtggctg tacgactttc cgccgtggtt tgagcagggg
    18601 gttatgttgt tcaagtacac cgatgctgat ggctatgact atggccttgg tctgccgctg
    18661 atcgccatga ttacggtggt gctgctgaca gcgtttatca tgaatttcac cagtgtaggg
    18721 cgcaaaattt atgcccttgg cgggaaccgc gaatcagcca gtcgcatcgg ctttagcgtg
    18781 ctgaaactgc aacttttcgt ctacggctat atgggattga tgtctggcgc tgcgggtgta
    18841 gtgcagtcgt ggacggtgat gactgtcgcc cccgattctc ttctgggtta tgagctgaca
    18901 gtactggctg cggtggtgct tggcggcact agtttgctcg gcgggcgcgg cacgttaact
    18961 ggtactttgc tcggcgtggt gttgttggca gtgatgcaaa acgggctaaa tttattggga
    19021 gtctcgtctt actggcaaac attgatcacc ggcatcatca tcgttgccag cattagcgcc
    19081 acggcgtgga gtcagcatca gaaccggagc ctgctatgaa aaaatcgtgg cgtaataacg
    19141 ttgagtttta cttgatcggc ctgctggtgc taactgttgc cgcgttcagc attactatgc
    19201 cggagatttt ctggtcgata agtaatttcc agtccgtcgc ttcgcaaatg cccgtgctgg
    19261 gcattctggc gttggctatg gcggtgacca tgctttgcgg tggcattaac ttgtcaatta
    19321 tagccactgc taacgcctgt tcactggtga tggcgtgggt ggcgactcag tatccaccgg
    19381 gtattgctac ggttgtcgct acgttactgg cgggcgctgg cgcggcggtg ataatcggtt
    19441 tatgtaacgg cgtattgatt gctggtattc gagtgttgcc aattcttgca acgcttggga
    19501 tgatgacgtt gctcaaaggg ataaatattc tggtgacagg tggtagcgcc attgccaatt
    19561 atccctcatg ggtgctgtgg ttgaatcatg cgcaatggtt cggtattccg ctaccgatgt
    19621 ggttgtttgc cgcagtggca ctggggctgt ggatattgct ggagaaaacg ccgctcggta
    19681 aagccattta cctgattggg tctaacgaac gagcaacact ttactccggc attaacactc
    19741 gtcgggtgct gatttgggtt tatgtcattt ccgccttgct ttgtgcggta gcggcatttt
    19801 tgatgatgtc gaaactcaac tcggctaagg cctcttatgg cgaatcgtac ttgctggtgt
    19861 caatcctcgc ggcagtactc ggcggggtta atccggacgg cggcagcggg cggataattg
    19921 gtatggtgct ggcgctgttt cttctgcaaa ttattgaaag cggcttcaat attctcggta
    19981 taagccccta cctgacgatg gccttatggg gaacgttgct gctctgtttc attcaggctc
    20041 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
    20101 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
    20161 gaagatcaaa gctgttggtt catattccgc taaacaacca cttgaaccga tggatatcac
    20221 ccggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
    20281 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtgccggg
    20341 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
    20401 cgatttggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgaaga
    20461 cgggctggaa aactactgtg atcacatgac cggcacctat aactcgccga cgccggacga
    20521 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
    20581 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
    20641 caccacgtat tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
    20701 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
    20761 ggtggcattt accacttctg aggcaaaacg cgaagcggca aaagccctgg gggccgatga
    20821 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
    20881 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
    20941 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
    21001 cctgatcatg aaacgccgtg cgatagccgg ttctatgatt ggcggcattc cagaaactca
    21061 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
    21121 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
    21181 tatcgataat cgcacactaa cagactgaaa aaattaataa atacgctgtg gtttaacata
    21241 ttaacttcgc tctccactta actttttagt taaggagagc gaaataatat caaagtagca
    21301 gtaaaaccta taacgtaaat tcaaattgtt aaattaactc cctccagtac ctaatacttc
    21361 acacgttagt tatgagcgat ttctgatagt gcctggttta atcagagctt tattatctgc
    21421 gacgtttatt tttatttaag agagatcgcg atgatatcat taaaagctcc gcacaataat
    21481 ttgatgccat atacgcaaca aagcatactt aatacggtta aaaacaatca gttaccagag
    21541 gatatcaaaa gctccctggt ttcctgtgtc gatatattca aggttttgat taaacaatat
    21601 tatgattatc cttatgattg tcgtgatgat ttagtcgatg acgacaaact cattcatctc
    21661 atggctgccg tacgagactg tgagtggtca gacgataacg cactcaccat aaatgtgcag
    21721 tttaatgatt tccctggatt ttatgactgg atggattacc ctgatcaccc ggttaagttt
    21781 gtttttcgca tacttgaaaa tcaaaaaggg acagtttggg tatatgacca ggatgatgcg
    21841 tttcttgata ttaaagcaaa tgtgcaggct ggacgcttta ccgggcttaa gaaattagtg
    21901 cagtttatca attcagtgcg aacagattgc aaatgtattc ttcttgaaca ccatatgcct
    21961 ttgctgagac ttttccctaa agggaaagag tgtatgcatg tggaaaaatg gcttcgcgaa
    22021 atgtcgtcta ttccggagac agacgcgccc attaagcagg cgcttgcgca tggattatta
    22081 ttacacttaa aaaatatcta ccctgttttt cctgaatcac tggtgatgtt attgctctct
    22141 gttctggacg taaaaacata cagagatgat gcacgattaa atgaagggat atctaataga
    22201 gtccaggaat taggtgacag atattatcct gtaaataagc acgttaaaat ccgctacact
    22261 ctttagttca gaaagggttc cacctgcaag ttgttattct acctgagagt gaatcgcaat
    22321 gcaggtaatc atttcatctg tatatctgta tgccaactgg cattccatct cttttttcgc
    22381 cgatattttg ttgtgatcgg cgacacttcg cttaaaaaag caccagtagt ggtttcgcag
    22441 ccatgcggtg tataaaaaaa aatgatctca tgcagatgtt ttgtgaatgt gtgggttgac
    22501 attcatatga aaaaaatcat aattccatca tgtttgtgtg gggtcttttc tgtatcttac
    22561 gcatcgcact caagcctgac agaaaatatg ctgtaaggct catatcaaaa accgccatta
    22621 gctcatcagg aagagcagac gacaaccagg attgttgtat ggtacggggt tcgaggcccc
    22681 gatggcggtc caacttttca aaactcttct gaattagatc cccttattat catccctgca
    22741 aagtggaaca ttgccattgt ttggctattc aggacgggtc agaccaatga aaaaagccca
    22801 cacaggggag agtgggctga aatgggaagc taaagactca agtaaactta tcggaaataa
    22861 ggaccacgca ttacgggggc tatcatcgcc gatggtcggg attcggcaaa tcaggttgat
    22921 tacgtagaga gtaaattatc tgctcaccgc tgcgtcaccc cttcgtaaat caggcgtagc
    22981 gcgaatacac caataattgc accaataacc cgactggcaa cgcgttgcat acgcccataa
    23041 gcacgacgca cagcgggcaa agaaaacgcc tgactaagaa aaactcgcca gataattgat
    23101 gcgagcacaa tccccgccca ggccattaaa cgtgcccatg ttggtgtttc ggcatttaat
    23161 gttactgaga aaatactaat aaaaaataaa acggtttgtg ggttagagag atcggtaatt
    23221 aatccacggc gaaaaaagac ataccagggg gcgttaatcg gttgttgtag tgtgctcatt
    23281 tgcggtgttg actggcggcg catgctgcac cacgcaaacc ataagagata agcgccgccg
    23341 acgattctga taagcgaaaa aatctcctca cactgcgtaa ttagcgttgc aagaccaaac
    23401 aaacccaacc cggaataaaa tgcatcgccc agcgccacgc ccagtccggt cagcactcct
    23461 gcgcgtcgac cggaagccag gctggtttgt actaccacaa aaagattggc tcccggatta
    23521 aaaaaagtaa tcacgaacag tcctacggtc aggtaaacgg catgcaaagg atccatagtg
    23581 atttcatcca taaataagtg aactaactgc atcatatttc taccaaaaat aatcgggtgc
    23641 gagagagatc acaaagtgtc ttatttccgg ttactggcgt ttatgccctg actgaactaa
    23701 ttattaatca acccaataat gtcggtgggt gatagtgtga taacaactct ggagccgtaa
    23761 tatgaaaata atctctaaaa tgttagtcgg tgcgttagcg tttgccgtta ccaatgtcta
    23821 tgccgctgaa ttgatgacca aagcggaatt tgaaaaagtt gaatcgcagt atgaaaaaat
    23881 aggtgatatt tcaaccagta atgaaatgtc gactgcagat gcaaaagaag atttaatcaa
    23941 aaaagccgat gaaaaagggg ctgatgtgtt ggtactgacc tccggtcaaa ctgacaataa
    24001 gatccacggc acggcggata tttataagaa gaagtaattc tgaatcctat gtaaacatcc
    24061 ccgatgcgta agtttatcgg tgatcatcta ttgaaattta tgccggataa agcgttcgcg
    24121 ctgcattcgg cagttcagct tttcagccgc cgccagaacg tcgtccggct gatgcctaaa
    24181 taattcgccg ctgctgtttt atcgccatta aatttctcca gtgcctgttg tggtgtcagc
    24241 aagcgtggag cgggaatttt cgccgactcg cgcgccagtt ccggcagtag cagctgcaaa
    24301 aactgcggcg ttaaatccgg cgtcggttcc acacttaaaa atagcgccag tcgctccatc
    24361 atattgcgca gttcacgaat attacctggc cagtcgtagt gcaccagcac ggtttcgctt
    24421 gcctgtaatc cctggcgtaa tgcggcagaa aatggggcgg agagcgccgc cagagacact
    24481 ttcaaaaagc tttccgccag cggaagaata tctgccaccc gctcgcgcag tggtggcaat
    24541 tgcagacgca aaatactcag ccgataaaac agatcacggc gaaactgccc ttgccgcata
    24601 tcttcttcca gattgcagtg agtggcgcta atgacccgta catctaccgg aacaggctga
    24661 tgcccgccga cgcgggtgac ctctttttct tccagcaccc gtaacaggcg agtctgcaaa
    24721 ggtagcggca tttcgccaat ctcatccaga aacagcgtgc caccgtgggc aatttcgaac
    24781 agcccggcgc gacctccgcg tcgcgagccg gtaaacgccc cttcctcata gccaaacagc
    24841 tccgcttcca gcagcgattc ggcaatcgcc ccgcagttga cggcaacaaa cggatgcgac
    24901 tttttgccct gtcgcgcatc gtggcgggca aaatattccc gatgaattgc ctgggccgcc
    24961 agctctttgc ccgtccccgt ttccccctca atcaacaccg ccgcactgga gcgggcatac
    25021 agcaaaatag tctgccgcac ctgctccatc tgtggtgatt gaccgagcat atcgcccagc
    25081 acgtaacgag tacgcagggc gttgcgggtg gcatcgtgag tgttatggcg taacgacatg
    25141 cgcgtcatat ccagcgcatc gctgaacgcc tggcgcacgg tggcggcgga atagataaaa
    25201 attccggtca ttccggcttc ttctgccaga tcggtaatca gccccgcgcc gaccaccgct
    25261 tcggtgccgt tagcttttag ctcgttaatc tgcccgcgtg cgtcttcttc ggtaatgtag
    25321 ctacgttgat cgaggcgcaa attaaaggtt ttttgaaacg ccaccagtgc cggaatagtt
    25381 tcctgataag tgacaacgcc gatagaagag gtgagttttc cggcttttgc cagtgcctgt
    25441 aacacatcgt agccgctcgg tttaatcaaa ataactggca ctgacaggcg gcttttcagg
    25501 tacgcgccgt tagagccagc ggcgatgatg gcgtcacagc gttcgtttgc cagtttcttg
    25561 cggatgtagg tcactgcttt ttcaaagcca agctgaatag gggtaatgtt cgccaggtga
    25621 tcaaactcga ggctgatatc gcgaaacagc tcgaataagc gcgttacaga taccgtccag
    25681 ataaccggtt tgtcgtcatt cagccgtggt ggatgtgcca tagcgcaccg caaagttaag
    25741 ataccgaata ttgggtttag tcttgtttca taattgttgc aatgaaacgc ggtgaaacat
    25801 tgcctgaaac gttaactgaa acgcatattt gcggattagt tcatgact
//
LOCUS       DAZKIF010000066        25517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZKIF010000066 DAZKIF010000000
VERSION     DAZKIF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.066"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(219..2036)
                     /gene="malZ"
                     /locus_tag="ACHOTW_002660"
     CDS             complement(219..2036)
                     /gene="malZ"
                     /locus_tag="ACHOTW_002660"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP9844739.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(2192..3565)
                     /gene="proY"
                     /locus_tag="ACHOTW_002661"
     CDS             complement(2192..3565)
                     /gene="proY"
                     /locus_tag="ACHOTW_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP9844740.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(3641..4960)
                     /gene="brnQ"
                     /locus_tag="ACHOTW_002662"
     CDS             complement(3641..4960)
                     /gene="brnQ"
                     /locus_tag="ACHOTW_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP9844741.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(5367..6662)
                     /gene="phoR"
                     /locus_tag="ACHOTW_002663"
     CDS             complement(5367..6662)
                     /gene="phoR"
                     /locus_tag="ACHOTW_002663"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP9844742.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            complement(6720..7409)
                     /gene="phoB"
                     /locus_tag="ACHOTW_002664"
     CDS             complement(6720..7409)
                     /gene="phoB"
                     /locus_tag="ACHOTW_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP9844743.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            7599..8801
                     /gene="sbcD"
                     /locus_tag="ACHOTW_002665"
     CDS             7599..8801
                     /gene="sbcD"
                     /locus_tag="ACHOTW_002665"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP9844744.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8798..11941
                     /gene="sbcC"
                     /locus_tag="ACHOTW_002666"
     CDS             8798..11941
                     /gene="sbcC"
                     /locus_tag="ACHOTW_002666"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP9844745.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            12067..13251
                     /gene="araJ"
                     /locus_tag="ACHOTW_002667"
     CDS             12067..13251
                     /gene="araJ"
                     /locus_tag="ACHOTW_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP9844746.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(13394..14302)
                     /gene="mak"
                     /locus_tag="ACHOTW_002668"
     CDS             complement(13394..14302)
                     /gene="mak"
                     /locus_tag="ACHOTW_002668"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP9844747.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            14427..15338
                     /gene="rdgC"
                     /locus_tag="ACHOTW_002669"
     CDS             14427..15338
                     /gene="rdgC"
                     /locus_tag="ACHOTW_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP9844748.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(15423..15872)
                     /locus_tag="ACHOTW_002670"
     CDS             complement(15423..15872)
                     /locus_tag="ACHOTW_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844749.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(15869..18148)
                     /locus_tag="ACHOTW_002671"
     CDS             complement(15869..18148)
                     /locus_tag="ACHOTW_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFP9844750.1"
                     /translation="MNRINILVICMVLFFMTGNACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADV
                     NKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVAD
                     ELINNEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTE
                     DIERIAEQMYLDNKENKNIVKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISY
                     LLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNG
                     VVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVV
                     SMKNSTRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRN
                     AHTPASLLTTLTESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVK
                     TGATRKIRNEARHRLEEKQ"
     gene            complement(18292..18576)
                     /gene="ppnP"
                     /locus_tag="ACHOTW_002672"
     CDS             complement(18292..18576)
                     /gene="ppnP"
                     /locus_tag="ACHOTW_002672"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP9844751.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(18648..19325)
                     /gene="aroM"
                     /locus_tag="ACHOTW_002673"
     CDS             complement(18648..19325)
                     /gene="aroM"
                     /locus_tag="ACHOTW_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP9844752.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(19583..19774)
                     /gene="yaiA"
                     /locus_tag="ACHOTW_002674"
     CDS             complement(19583..19774)
                     /gene="yaiA"
                     /locus_tag="ACHOTW_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP9844753.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(19824..20348)
                     /gene="aroL"
                     /locus_tag="ACHOTW_002675"
     CDS             complement(19824..20348)
                     /gene="aroL"
                     /locus_tag="ACHOTW_002675"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP9844754.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(20531..20989)
                     /gene="yaiI"
                     /locus_tag="ACHOTW_002676"
     CDS             complement(20531..20989)
                     /gene="yaiI"
                     /locus_tag="ACHOTW_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9844755.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            21109..21918
                     /gene="proC"
                     /locus_tag="ACHOTW_002677"
     CDS             21109..21918
                     /gene="proC"
                     /locus_tag="ACHOTW_002677"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9844756.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(21935..23050)
                     /gene="adrA"
                     /locus_tag="ACHOTW_002678"
                     /gene_synonym="dgcC"
     CDS             complement(21935..23050)
                     /gene="adrA"
                     /locus_tag="ACHOTW_002678"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP9844757.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(23152..23472)
                     /gene="psiF"
                     /locus_tag="ACHOTW_002679"
     CDS             complement(23152..23472)
                     /gene="psiF"
                     /locus_tag="ACHOTW_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP9844758.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(23591..25006)
                     /gene="phoA"
                     /locus_tag="ACHOTW_002680"
     CDS             complement(23591..25006)
                     /gene="phoA"
                     /locus_tag="ACHOTW_002680"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP9844759.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(25107..25367)
                     /gene="iraP"
                     /locus_tag="ACHOTW_002681"
     CDS             complement(25107..25367)
                     /gene="iraP"
                     /locus_tag="ACHOTW_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP9844760.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         6425 a         6763 c         6223 g         6106 t
ORIGIN      
        1 gtttcaataa attcgaattt gaaattgtca tatctttgct ccactacagg gttatcctgg
       61 agcatgttgt acataacatc atgggcaaac aataccaaaa atcgctcata tcgatcggga
      121 caaatattta acaaattcag ccctgacatt gagcagcttt tttgcgctgc gtagtgtcag
      181 ggctggtgac taacgacgta acaaataaaa cagatgcttt agttcatcca caccgtagcc
      241 gaaatggcag gcaaagccag aatcccgtct gtcagttgcc catggccttc tttgcgttgc
      301 cattgcacga cattaagaaa cggtgacgcg ggtagcacca cttcacaggc ttcgccacgg
      361 ttaattgcca ccagtacacg ctgctgattc agcacgcgga caaataccac cacgttatct
      421 tccgcataca gcacctgaca gccgccacga cgcagcgcct gacttttctt acgcagcgca
      481 atcattcgct ggtacaacgc gaataacgcc atatcctgtt tttccacctg ccaggggaac
      541 ggcttacggc aaaacggatc atttttgcca tccagtccca cttcatcacc gtaataaatg
      601 cacggtacac caggccaggt gaacagccag accactgcca ggggcaggcg cgcaacctcc
      661 cgaccgagca gcgttttaaa tcgcgcagta tcgtggctgt cgagctgatt aaacatacgt
      721 aattgctgtt gatgagaaag ccctgcgcgg taattatcca tccaggccat acaggtttga
      781 gcgtcaattt gctgcggatc gtaagagatg tcggtattgg caagaaatcc ccacaacggg
      841 aatgtgaagc cgcgatagtt catggcggca tcttccacat cggcctgtaa ccattgccgt
      901 gcatcgccaa aatgctctcc aactatatac gcttccggct gggtttcttt cgccgcttcg
      961 gtgatcccag caacgtgctg caaattattg cgcgccccac ccgcttcccc cagcatatgc
     1021 accacatcca gccgccagcc gtccatattc cacggcgctt tcagccagtg gcggacaata
     1081 ctgtcttccc cgcgataaat ttcattcacc agactttccg actgataatc cagcttcggc
     1141 aagctggcat agccaagcca gtcgagcgcc gtgccatcat cactaaacga gtaccagtcg
     1201 cgccagggcg attcggggtt gtgacaagct ccccccgtgc cacgattgtg cctgtcaaac
     1261 caggcatggg aatcgccact gtggttaaac acgccatcca gcaccagccg cattcccagc
     1321 tgctgcgtat tgtggcgtaa gcgcagcaac gccccatcac cgccaaactg cggatcgaca
     1381 tggcgataat cctcggtatc gtatttatgt acgctgggag ctttaaacac cggattgaga
     1441 tacagcgccg tcacgccaag ctttttcaga tacggcagtt tttcgcttat cccgtccaga
     1501 tcgccgccat agaacgttga tccgcccgcc tgcgccgtga ccggttcatc ccagtcacgc
     1561 aagatgatct cttgtccggc tgcatgatgg taatagacat gatcctgttc agcttcacga
     1621 ggaagactac gcgcaaaacg atcagggaag atctgataaa aaatctgatc cgcagcccat
     1681 tgtgggccga tatccggtac atcgacggca aactgctcca gtcgtgccgg cggcattcgg
     1741 ctgaagccct gcggtgtaaa ccaacgctgg cgatcgtgcc acagcagttt gaaactgtaa
     1801 cgccgccggg gttgtccgct ggagagatca atcgccgcac gccatgcggt gacgcctggc
     1861 tgcggctgac tgcgctgctt atgcattggt actgacattt cttcgttatc gtgttctgta
     1921 cgcagcataa tgcgctgcgg tgggtcttcg cccgtcagcc acagtgtaat gagcagttga
     1981 tctttgcttt gtttaacaaa tgggggcacc ggcaggtgcc atgcatttaa catcataatt
     2041 cccctgtgat gaaaatctga tcaccttgcc atagcctaat gcaggatcac tcatcatctg
     2101 aaggatattt gggggaggag gacgggggcg gataactttc ggaatagccg gatcaggatt
     2161 tcctcctgaa ccggcttaca gaacgcaagt attattggtt ttcagccagc tgacgatcgt
     2221 ggcgacgctt aaacatccag ccaatcaaca gcacaacaat ccacgcgaaa ccgacataca
     2281 gcgagatacg cgtatccggg tgataaccaa tcaacccgat aataaagagc aggaaaatca
     2341 gcccgccgat ggtcgttgct accccacccg gcactttaaa tttcagcgcc ttaacttctt
     2401 ctggcggcaa acggcgacgg aaggcaattt gcgacagcag gatcataatc cacacccaca
     2461 ccgtggcgaa ggttgccagc gaagcgatca ccaggaagac gttttccggc atgatgtagt
     2521 tcagatacac cgcaaacagc agcgcggtag tcatcaccag caccgtaacc cacggaatac
     2581 cgcgacgcga cgttttgctg aaaattttcg gcgcgctgcc ctgctctgcc ataccgtgga
     2641 gcatacggcc tacgccaaat acatcactgt taattgccga cagcgaagca gtcagcacaa
     2701 caaagttaag aatgctggcg gcaaaggtaa tgcccatatg ctggaacgtc agcacgaacg
     2761 ggctaccggc agtgccaacc tgattccacg ggtagataga cataatgacg aacagcgtac
     2821 cgacgtagaa caccagaata cgcatcggca cggagttaat cgcacgcggt atcgatttct
     2881 caggatcttt cgcttcaccg gcggtaatcc cgataatttc gatcccaccg taagcaaaca
     2941 tcaccatttg caacgacatt accatgccaa gccagccgtt actgaagaag ccgccgttgc
     3001 tccacaggtt atgaataccg gtcggttgcc cgccgttgcc aatcccccag atgatgatgc
     3061 cgaaaccggc gacaatcatg atgatgatgg tggcgacttt aaagaacgag aaccagaatt
     3121 ccagctcacc gaataccttc acgctcatca ggtttacggc gcaaatgatc agcaccacgc
     3181 tcagtaccca aatccagtgc ggcaccgtcg ggaaccagac acccatatag ataccaaaag
     3241 cggtcacatc ggcgatggcg acaataagga tttcaaagca gtaggtccag ccggtaatgt
     3301 aacctgccag cgggccgagg ttttcctgcg cataacgcga gaaagagctg gcggccgggt
     3361 tatgtaccga catttccccc agcgcacgca taatgatata cgccgcgata ccaccgataa
     3421 tataggccaa caacacgctc ggaccggcca ttttgatggc gtctgccgaa ccgtaaaaca
     3481 gcccggtgcc aattgctgaa cccagtgcca taaagcgtat gtggcgggta cttagcccac
     3541 gctttagctt gttcttactt tccatcaatt cctaacccat caacacaata aaaaaccacg
     3601 gggcatcgca gccccgtggt taaaacaaat gttcagtgat ttagtgagcg ctggaggtca
     3661 cctgacgacc tgccgcgcga tcccagataa tggccagaac caccatcacc actgttggca
     3721 ttaaccacgc cagaccttgt tcggccagcg gtaaacgctg cgcccaggac ggtaagatat
     3781 cgctgaatgc agatgctttg atcccgtcga gaataccaaa aagcaggctg ataaacatcg
     3841 gcggagcaat cacgcgggac gaattatgcc accatgagcg tgtaaaactt aatacaacca
     3901 gtgcgataca cggcggataa atagcggtca gcaccggtac ggagatctgg atcagctggc
     3961 ttaagccgag gttagaaacc accatcgaga agccgccgag gataaacacc agcgtacgat
     4021 aagagagcgg tacgtactgg gcaaagaatt ctgcacaagc acaggtcagg ccaactgccg
     4081 ttaccaggca ggcgatgaag attaacgccg ccagcaggaa gctaccgccg ccgccaaagg
     4141 tgtgctgaac gtaagcatgc agaatagcag cgccgtttgc agactgatcg accagcgacg
     4201 cgctgtctga acccagacgg aacagcgcca ggtacagcag agtcagacca acacccgcca
     4261 tcaggccagc ccagacggta taacgggtca gtaggcgcgc ttcggtaacg ccacgagaac
     4321 gcgccgcgtt aacaataacg ataccaaaca ccattgcgcc cagcgtatcc atggtcagat
     4381 agccgttgac gaagccgtta gaaaacgcag cgttttgata agcctcagtc gccgtgctga
     4441 tagaacccgc cggccagaca atcgcggcaa cagacaggat gaccagcgcg ataattttca
     4501 gcggcgcaag gaagttgccc acggtatcca gcagcttgcc cggatagagc gaaaccagaa
     4561 taacgatagc gaaatagacc aggctgtaga taaacagcgg cagcgcggaa tcacccgtca
     4621 gcggcgcaat ccccacttca aaagaaacgg tagctgtacg cggcgtagcg aaaagcggcc
     4681 ccaccgccag gtaacaaacc gttgccagca gtacgccagc gactttaccg attggcgtgc
     4741 tgaggctgtc aacaccgccg ccaacttttg ccagcgccac taccgttaat accggcaggc
     4801 caacggcagt aatgaggaag ccgaatgccg cagtccagac gtgttcgcct gcctgtaagc
     4861 cgaccattgg agggaaaata atgttacctg cgccgacgaa caacgcaaat gtcataaagc
     4921 ccagagcgat gatatcgcgc gatcttaatt gatgggtcat aaatcatact gcctgtggat
     4981 gtggtgttgt aacgttaaat ttttgtgcca atccctgggg atgatacagc ggtatattta
     5041 aacaatttta gcgggaaata ctcccaacca ggcgcggcga agaatagttt ttcgatttac
     5101 cgcgttaatg cagtcagtat gacagcacct gaagcgcaat ttaaacgctt ataacgtttt
     5161 aaagcaagga gggatctaaa aaccagaggt atatagcgat aagaaatcac caaccagtat
     5221 aaaacactat ttccgtgaaa aagacatggc taatcatgcg aacaaaaaat caccaaccgt
     5281 tgtaaaatct aacgcccgta ttttgactgc aaagaaaata agccagcatc ggctggctta
     5341 tggcaataaa agatgacaaa ggcggattaa tcgctgtttt tgacaattaa acgttccggg
     5401 ataacaaaac tgaaacgtgt tccttttcct actgtactct caatattcag gcgactttcg
     5461 tgatgattca ccgcatgttt cacgatcgct aaccctaatc cgctaccgcc ggtttgccgg
     5521 gaacgcgctt tatcaacgcg ataaaaacgc tcggtcaggc gcggaatatg ctccggtgca
     5581 atgcccggtc cgttatcttc aacgctaaat tcggcaccgt gcggcactcg ctgccagcgt
     5641 acggtgatat gcgtgccttc cggcgtatga ttcacggcgt tatagaccag gttcgaaatc
     5701 acactgcgta gctgatcttc gttgccagac accttgaggc cgttatctat ctcgaaggta
     5761 aatgtctgtt ttttctgact cagagtctga gcctcgcgct caacaacgcg cagcatcatc
     5821 ggcacatcaa ccttttcatt gagcaattgc gtcggtgcgg cttcaatttt cgacagcgtt
     5881 agcaactgct tcaccagccc ttccatccgc tgggtctgct cgcgcatggt gtgcaacgct
     5941 ttttcgcgta ccgcgccttc cagcggctgc tcatccatca tctccaggta accctgtaac
     6001 acggtcaatg gcgtacgtaa ctcatggctc acgttggcaa aaaagttacg ccgcgcccct
     6061 tccagttgat gcatttgcgt gacatcacgc gccaccatca gcaactgttt gtgggtataa
     6121 ggcatgacgc gaatttccag atgccgcccg gtgttgagca ccagattgag cgggcgagaa
     6181 aaatcacgcg ttttcaaata ttgcgtaaac tccgggtaac gcagcaggtt aaggatgttc
     6241 tgcccgttat cttccggcca gcgcaaacca agaatctgtt gcgccagacc gttacaccag
     6301 aaaataccgc cctcttccgt ggtcagcacc accgcgtcgg gcagcgactc cgcgccgcta
     6361 cgaaagcgtt taatcagatt gcccagttca cggcggcgtt ttttatttcg cagctgcatc
     6421 tggtgtaagc cgtatagtag cggttcccag ctaccacgcc ccggtggcgg ggtcatactg
     6481 cgatccaccc acagccacca tgaaaggcgc aataaattcc agaaatgcca gataagcagt
     6541 cctgttaccg atgccagcaa aaaccagggc aggtaaccaa aaaatgcacc caggatgaaa
     6601 gccgggaggc agcaaagtag cagctccagc accagccttt tccacgacag ccgttccagc
     6661 acgtaagata ctccagttaa gaaatcataa gccctgctct gcgtccgatg agcaaggcgt
     6721 taaaagcggg ttgaaaaacg atatcctgta ccgcgcacgg tctgcaccat gcggtcatgc
     6781 ccgccgggct ccagtgcttt acgcaggcga cgaatgtgga catcgaccgt gcggtcttcc
     6841 acatacacgt tagttcccca gacgtggttt aacagctgct cgcggctgta cacgcgctca
     6901 ggatgcgtca taaaaaagtg cagcagttta aattctgtcg gccccatctc cagcggctct
     6961 tcgcccgcca tcactcggtg agatgtcggg tcgagactta atccctgcat ctcaatcacc
     7021 tcttccaccg ccattggcga aatacggcgc attaccgctt tgattcgcgc caccagctcc
     7081 ttcggcgaaa acggcttggt gatatagtca tccgcgccgg tttcaaggcc gcgcacgcga
     7141 tcttcttctt cccctctggc ggtcaacatc accactggaa tatcccgggt catcgactcg
     7201 cgcttgaggt gtttgatgaa ctggataccg gagccgccag gtaacatcca gtcaaggaga
     7261 attaaatccg gccagggttc attcagttga ttcacagcac tgtcataatc ttccgcttcg
     7321 accggctgaa agccattttg ttcgagcacg aagcagacca tttcgcgaat tggagcttca
     7381 tcttctacga ccagaatacg tctcgccatg atttgccctg ttgtaataaa taggttgcga
     7441 tcattaatgc gacgtcatta tgcgtcagat ttatgacaga tttatgaaaa gctcgtcgca
     7501 catatcttca ggttattgat ttccgtggcg cagaaaaaag caaatggcac atctgtttgg
     7561 gtataatcgc gcccatgctt tttcgccagg gaaccgttat gcgcatcctt cacacctcag
     7621 actggcatct cggccagaac ttctacagta aaagccgcga agctgaacat caggcttttc
     7681 ttgactggct gctggagaca gcacaaaccc atcaggtgga tgcgattatt gttgccggtg
     7741 atgttttcga taccggctcg ccgcccagtt acgcccgcac gttatacaac cgttttgttg
     7801 tcaatttaca gcaaactggc tgtcatctgg tggtactggc aggaaaccat gactcggtcg
     7861 ccacgctgaa tgaatcgcgc gatatcatgg cgttcctcaa tactaccgtg gtcgccagcg
     7921 ccggacatgc gccgcaaatc ttgcctcgtc gcgacgggac gccaggcgca gtgctgtgcc
     7981 ccattccgtt tttacgtccg cgtgacatta ttaccagcca ggcggggctt aacggtattg
     8041 aaaaacagca gcatttactg gcagcgatta ccgattatta ccaacaacac tatgccgatg
     8101 cctgcaaact gcgcggcgat cagcctctgc ccatcatcgc cacgggacat ttaacgaccg
     8161 tgggggccag taaaagtgac gccgtgcgtg acatttatat tggcacgctg gacgcgtttc
     8221 cggcacaaaa ctttccacca gccgactaca tcgcgctcgg gcatattcac cgcgcacaga
     8281 ttattggcgg catggaacat gttcgctatt gcggctcccc cattccactg agttttgatg
     8341 aatgcggtaa gagtaaatat gtccatctgg tgacattttc aaacggcaaa ttagagagcg
     8401 tggaaaacct gaacgtaccg gtaacgcaac ccatggcagt gctgaaaggc gatctggcgt
     8461 cgattaccgc acagctggaa cagtggcgcg atgtatcgca ggagccacct gtctggctgg
     8521 atatcgaaat cactactgat gagtatctgc atgatattca gcgcaaaatc caggcattaa
     8581 ccgaatcatt gcctgttgaa gtattgctgg tacgtcggag tcgtgaacag cgcgagcgtg
     8641 tgttagccag ccaacagcgt gaaaccctca gcgaactcag cgtcgaagag gtgttcaatc
     8701 gccgtctggc actggaagaa ctggatgaat cgcagcagca acgtctgcag catcttttca
     8761 ccacgacgtt gcataccctc gccggagaac acgaagcatg aaaattctca gcctgcgcct
     8821 gaaaaacctg aactcattaa aaggcgaatg gaagattgat ttcacccgcg agccgttcgc
     8881 cagcaacggg ctgtttgcca ttaccggccc gaccggtgcg gggaaaacca ccctgctgga
     8941 cgccatttgt ctggcgctgt atcacgaaac tccgcgtctc tctaacgttt cacaatcgca
     9001 aaatgatctc atgacccgcg ataccgctga atgtctggcg gaggtggagt ttgaagtgaa
     9061 aggtgaagcg tatcgcgcgt tctggagcca gaatcgggcg cgtaaccaac ccgacggtaa
     9121 tttgcaggtg ccacgcgtag agctggcgcg ctgcgccgac ggcaaaattc tcgccgacaa
     9181 agtgaaagat aagctggaac tgacagcgac gttaaccggg ctggattacg ggcgcttcac
     9241 ccgttcgatg ctgctttcgc aggggcaatt tgctgccttc ctgaatgcca aacccaaaga
     9301 acgcgcggaa ttgctcgagg agttaaccgg cactgaaatc tacgggcaaa tctcggcgat
     9361 ggtttttgag cagcacaaat cggcccgcac agagctggag aagctgcaag cgcaggccag
     9421 cggcgtcgcg ttgctcacgc cggaacaagt gcaatcgctg acagcgagtt tgcaggtact
     9481 tactgacgaa gaaaaacagt tacttaccgc gcagcagcaa gaacaacaat cactaaactg
     9541 gttaacgcgt ctggacgaat tgcagcaaga agccagccgc cgtcagcagg ccttgcaaca
     9601 ggcgttagcc gaagaagaaa aagcgcaacc tcaactggcg gcgctcagtc tggcacaacc
     9661 ggcacgaaat cttcgtccgc actgggaacg catcgcagaa cacagcgcgg cgctggcgca
     9721 tactcgccag cagattgaag aagtaaatac tcgcttacag aacacaatgg cgcttcgcgc
     9781 gagcattcgc caccacgcgg cgaagcagtc agcagaatta cagcagcagc aacaaagcct
     9841 gaatacctgg ttacaggaac acgaccgctt ccgtcagtgg aacaacgaac tggcgggttg
     9901 gcgtgcgcag ttctcccaac aaaccagcga tcgcgagcat ctgcggcaat ggcagcaaca
     9961 gttaacccat gctgagcaaa aacttaatgc gcttgcggcg atcacgttga tgttaaccgc
    10021 cgatgaagtt gctaccgccc tggcgcaaca tgctgagcaa cgcccactgc gtcagcgcct
    10081 ggtcgcgctg catgggcaga ttgttcccca acaaaaacgt ctggcgcagt taatggtcac
    10141 tatccagaat gtcactctgg aacagacgca acgtaatgcc gcacttaacg aaatgcgcca
    10201 gcgttataaa gaaaagacgc agcaacttgc cgatgtgaaa accatttgcg agcaggaagc
    10261 gcgcatcaaa acgctggaag ctcaacgtgc acagttacag gcgggtcagc cttgtccact
    10321 ttgtggttcc accagccatc cggcggtcga ggcgtatcag gcgctggagc ctggcgttaa
    10381 tcagtctcga ttactggcgc tggaaaacga agttaaaaag ctcggcgaag aaggtgcggc
    10441 gctgcgcggg caactggatg cattaacgaa gcagcttcag cgcgatgaaa acgaagcgca
    10501 aagcctccga caagatgagc aagcacttac tcaacaatgg caagccgtca cggccagcct
    10561 caatatcacc ttgcagccac aggacgatat tcaaccgtgg ctggatgcac aagatgagca
    10621 cgaacgccag ctgcggttac tcagccaacg acatgaatta caagggcaga ttgccgcgca
    10681 taatcagcaa attatccagt atcaacagca aattgaacaa cgccagcagc agctcttaac
    10741 ggcattggcg ggttatgcac tgacattgcc acaggaagat gaagaagaga gctggttggc
    10801 gacacgtcag caagaagcgc agagctggca gcaacgccag aacgaattaa ccgcgctgca
    10861 aaaccgtatt cagcagctga cgccgattct ggaaacgttg ccgcaaagtg atgatctccc
    10921 gcacagcgaa gaaactgtgg cgctggataa ctggcggcag gttcatgaac aatgtctcgc
    10981 attacacagc cagcagcaga cgttacagca acaggatgtt ctggcggcgc aaagtctgca
    11041 aaaagcccag gcgcagtttg acaccgcgct acaggccagc gtctttgacg atcagcaggc
    11101 gttccttgcg gcgctaatgg atgaacaaac actaacgcag ctggaacagc tcaagcagaa
    11161 tctggaaaac cagcgccgtc aggcgcaaac gctggtcact cagacagcag aaacgctggc
    11221 acagcatcaa caacaccgac ctgacgggtt ggctctcact gtgacggtgg agcagattca
    11281 gcaagagtta gcgcaaactc accaaaagtt gcgtgaaaac accacgagtc aaggcgagat
    11341 tcgccagcag ctgaagcagg atgcagataa ccgtcagcaa caacaaacct tactgcagca
    11401 aattgctcaa atgacgcagc aggttgagga ctggggatat ctgaattcgc taataggttc
    11461 caaagagggc gataaattcc gcaagtttgc ccaggggctg acgctggata atttagtcca
    11521 tctcgctaat cagcaactta cccggctgca cgggcgctat ctgttacagc gcaaagccag
    11581 cgaggcgctg gaagtcgagg ttgttgatac ctggcaggca gatgcggtac gcgatacccg
    11641 taccctttcc ggcggcgaaa gtttcctcgt tagtctggcg ctggcgctgg cgctttcgga
    11701 tctggtcagc cataaaacac gtattgactc gctgttcctt gatgaaggtt ttggcacgct
    11761 ggatagcgaa acgctggata ccgcccttga tgcgctggat gccctgaacg ccagtggcaa
    11821 aaccatcggt gtgattagcc acgttgaagc gatgaaagag cgtattcctg tgcagatcaa
    11881 agtgaagaag atcaacggcc tgggctacag caaactggaa agtacgtttg cagtgaaata
    11941 actattcagc aggataatga atacagaggg gcgaattatc tcttggcctt gctggtcgtt
    12001 atcctgcaag ctatcacttt attggctacg gtgattggta gccgttctgg tagttgtgat
    12061 ggtggtatga aaaaagtcat tttatctttg gctctgggca cgtttggttt ggggatggcc
    12121 gaatttggca ttatgggcgt gctcacggag ctggcgcata acgtaggaat ttcgattcct
    12181 gctgccgggc atatgatctc gtattatgcg ctgggggtgg tggtcggtgc gccaataatc
    12241 gcactctttt ccagccgcta ctcactcaaa catatcttgt tgtttctggt ggcgttgtgc
    12301 gtcattggca acgccatgtt cacgctctct tcgtcgtacc tgatgcttgc cattggtcgc
    12361 ctggtttccg gctttccgca tggcgcattt tttggcgtcg gagcgatcgt gttatcaaaa
    12421 attatcaaac ccggaaaagt caccgccgcc gtggcgggga tggtttccgg gatgacagtc
    12481 gccaatttgc tgggcattcc gctgggaacg tatttaagtc aggaatttag ctggcgttac
    12541 acctttttat tgatcgctgt ttttaatatt gcggtgatgg catcggtcta tttttgggtg
    12601 ccggatattc gcgacgaggc gaaaggaaag ctgcgcgaac aatttcactt tttgcgcagc
    12661 ccggccccgt ggttaatttt cgccgccacc atgtttggca acgcaggtgt gtttgcctgg
    12721 ttcagctacg taaagccata catgatgttt atttccggtt tttcggaaac ggcgatgacc
    12781 tttattatga tgttagttgg gctagggatg gtgctgggaa atatgctaag tggcaggatt
    12841 tcaggacgtt attcaccact gcgcattgca gcagtgactg actttataat tgtactggca
    12901 ctgctgatgc tctttttctg cggcggcatg aaaacaacgt cgcttatttt tgcttttatt
    12961 tgttgcgcgg gattatttgc cctttcagca cctctgcaaa tattgttact gcaaaatgcc
    13021 aaaggcggag agttattagg tgccgcaggt gggcaaatag cgtttaacct cggtagcgcc
    13081 gtcggcgcat attgcggagg tatgatgctg acgctggggc tggcatataa ttacgtggcg
    13141 ctgcctgccg ccctgctttc gtttgctgcg atgtcatcgt tgctgctgta tggtcgctat
    13201 aagcgccagc aagcggcgga tagtccggtg ctggcgaaac cactggggta gattactgtc
    13261 acggtgtgcg ttgcttattg ccggatgcgg cgtgaacgcc ttatccgccc tacgcggttc
    13321 tggcacattt tgtaggcctg ataagacgcg gcaagcgtcg catcaggcat gatgcgccaa
    13381 ttgcctgggt tttttactct tgtggccata accacgcagc gccgcgtacg ccgctggaat
    13441 caccgtgctt cgccttacgc accggcgttt cacattcgcc gccgaagaca aattgtttaa
    13501 tcaactgcgg aaccgtttga tataaacggt ctacattgct catcccgccc cccaggacaa
    13561 tcacatccgg atcgagaata ttcacgacat gtgccagcga ttttgccagc cgcagctcgt
    13621 agcgacgcaa tgccagttcc gctaccggat cgctttcttc aaccaggcgg ataatttcac
    13681 tgcctttcag cgcatgtccg ctcaaacgac gataatccgt cgcgaatccc gtgcccgaaa
    13741 taaaggtttc aatacaacct tgtttaccgc aataacaagg gacttcctcg cgataacgca
    13801 gttcgtcttc gtccatccac ggtagcggat tgtgtcccca ctcacctgcc gtgccattgc
    13861 cgccgatatg cgcccgccca ttgaatgcca cgcccgcgcc gcatcccgtg ccgataatca
    13921 cggcaaatac cgtctgcgct cccgctgccg cgccatctac tgcttctgaa accgccagac
    13981 agttggcgtc atttgccagc cgcacttccc gctgcaacct cgcgcttaag tctttatcga
    14041 atggctgacc gttgagccag gttgaattgg cattcttcac cacaccggtg taaggcgaaa
    14101 ttgagccagg aatgcccata cctaccgttc cgcgctgccc cgtcgcctgc tccgccatat
    14161 caaccaacgt ggcgatcgtt tcaatagtct gccggtaatc atcacgcggc gtgggcagac
    14221 gatggcggta caactgctcc cctgcatcgc ccagtgcaat cacttcagtt ttggtgccgc
    14281 ctaaatcgat acctatacgc acggtactct ccttattttt ttcaatatca atagcgtaga
    14341 gacggacaac cggattggca atgcaaggcc gccgacaatt cgttatcatg cccgctaaat
    14401 ttaacgacaa ggccgtggaa attatcatgc tgtggttcaa aaatttaatg gtttaccgtc
    14461 ttagccgcga gatttcgctg cgtgcagaag agatggaaaa acagctagcc tcgatggcat
    14521 ttaccccatg cggcagccag gacatggcga agatgggctg ggttcctccg atgggatcgc
    14581 acagcgatgc gttaacgcac gttgccaatg gtcaaattgt tatctgcgcg cgcaaagaag
    14641 aaaaaatcct accgtctccg gtgattaaac aggcgctgga agcgaaaatc gccaaactgg
    14701 aagcggaaca ggcgcgtaag ctgaagaaaa ccgaaaaaga ttcgctgaaa gacgaagtgc
    14761 tgcactctct gttgccgcgt gctttcagcc gttttagcca gacaatgatg tggatcgaca
    14821 cggttaacgg tttgattatg gtggactgcg ccagtgccaa aaaagcggaa gatacgctgg
    14881 cactgctgcg taaaagcctg gggtcgttac cggttgtacc attgagcatg gcaaacccga
    14941 ttgaactgac gctgaccgaa tgggtacgtt ccggtagtgc ggcacagggc ttccagctgc
    15001 ttgatgaagc cgagctgaaa tcgttgctgg aagatggcgg agtgatccgc gcgaagaaac
    15061 aagatctgac cagcgaagag atcaccaatc acattgaagc cggaaaagtg gtgactaaac
    15121 tggcgctcga ctggcagcag cgcattcagt ttgtgatgtg cgacgatggt tcgctcaagc
    15181 gtctgaagtt ctgcgacgag ctgcgcgatc aaaacgaaga tatcgaccgt gaagatttcg
    15241 cccagcgttt tgatgccgat ttcatcctga tgactggtga actggcagcg ttaattcaaa
    15301 acctgattga aggattaggt ggcgaagcac aacgttaatt gctgattttc ctttaatgcc
    15361 ggatgcgacg cctgccgcgt cttatccggc gtacgaagcc acaccaggca tataattatt
    15421 cgctacggcg ggcaataatt ttttagcgca gcaatattat gcgttttacg ccgtaactga
    15481 tctaatcgcc tcatccctat tcgcctgtca gcagcacgca gaatattcac taacccccat
    15541 ttatcgctgg taaattgttt tgttaacaac tccgcttttg gggtgtcaat atattcacgc
    15601 aataaatcag aaattgaaca aatatccgtc aggtaaggat accagcaagt ttcaccgtga
    15661 aaattacgca ctccaccgtt gcctgcaata aagtctttcg ggtaatccca gatcacgttt
    15721 ttatccaacg tgatccaata acgcggtaaa tcggtgcttc caccattttg gctgcgcatg
    15781 ggataacggg tacagtgaat ttgcaggttg atcgttggcg tcagtaagtc atagatctcc
    15841 cgctgtaact tgctccaggg gcgttgtgtc attgtttttc ctcaagccgg tgacgcgctt
    15901 cattgcgaat tttccgcgtt gccccggttt tcactaaatc ccgtaactgc gaaagatcgg
    15961 gtttatcgac aaataaaagt aatgatggat cctcttttag ccacaccgtt ttcacatcgg
    16021 cagccagctg tggattatta atagccagcg accgatcctg agactcagtc aatgtcgtta
    16081 acaacgacgc cggggtatgt gcgttgcgta atgccgtttg gcgcaaatga tcattgcgac
    16141 tttgcagcaa tgcacgccac cattcatcat ctgatttatc ttttatcgtc gccagttccg
    16201 caaaaatatc gctatcctca tggttccagc ggtgtaatcc tctttcactt tgtctacggg
    16261 tgctattttt catactgaca acagccagcg cccagtcaga gttgaagcga tactgttgat
    16321 cgagtttgtt gataattccg gaaggtaatt ctttattatc aaataattgg ttacgcattc
    16381 gatagcggta ttcagcgggt aaatcaacca gtaaatcaat ggcattattt aacatctctt
    16441 cgtcaacaac accattatca atcagcgcca gctgtacttc gtaggcttgc tcttgatctt
    16501 cttgccgttt gctcatcatt aaatttaatg tgcgttgcca taactgtttc ttatattcca
    16561 gcggcaataa tttatcgcgt gccagctcat cccagacggt gggaacatca tgctgagtca
    16621 atagataact aatcacgtcc ttcgatgtca gataacgcgc accttcgcgc aaattgtgca
    16681 catcttcttt tgccagagac agttgacgca tctccggcaa cgctatcaat aacaccttta
    16741 cgatattttt attttcttta ttatccagat acatttgttc tgcgatacgt tcaatatcct
    16801 ctgtacgcag ggttgctgac agttgggtaa tttttaattc agtaagcgtc cgggctaatg
    16861 cttcacgaac tttcagtgat ttatctttac tcaacatcac tgcttgttgt gcggttaaat
    16921 ctgctccggt aattctttca cgtaactccg ggtcttcgtt atttatcaac tcatcggcca
    16981 ctgcagtcgg aaccagccag gcgtaatcgt aatggctgag taatgtcttg cgtacactga
    17041 gaaaggggct atgacttaac gacgcaacaa tatctggaga aagattatcc tgtctggcct
    17101 gctccagctg tacgctctcg acgggtacag acattaattt tttattcaca tcggcggaaa
    17161 tattactgtt tttaaccagc cctctggcaa cagcctcatc tttaattaat tcatcggtaa
    17221 gccaatgcat ctgttgagcg ggccaggact gcgccatcga ttcacgcgtc tggtcatcgg
    17281 ggtgctgtaa cagttgcagc ataacggcgg gtgataaact ctctgtatga gtcagaaaac
    17341 gtgccaccag tggatcttga tgctttattt tattcgctgg gaaattatcg agtttccccg
    17401 tttcaatcca ttttaatttc tcattaattt caggagcggc tttatctcga aaaagcggta
    17461 aaccctgaac gatgttgtct gcgacgctat catagtcagg atacgcattg gaaatgttat
    17521 cggtttcgat ttcagtgtga tcataaagat cggcagtcac cagtgaatag gctttttgaa
    17581 tattctcatc ccagttatat ccttcaccac ttccatcttc attgatataa aattcagctt
    17641 taatgacttc aaattgcgtt gctttatcgg aaacaataat gcctcgcgca ctaattccct
    17701 tcatcacttc aaagttatga tcattgtaat cagcataaac taaacctttc gctgtcatct
    17761 gtcctttgac atacgcgaaa tcatgattaa taaagttatc gacaataaca ttacctaata
    17821 caatcagatg accattacct gacagataat catcataatc gccggaaatc gttaaattac
    17881 cgttcaccac cagtttattt ttattgctga agttagcggg taatgatagg tcaccattat
    17941 gataatacca gcggatatta tcttttagca tataagcagc ttccatacta tcgtcggttg
    18001 ccgctttaac cacgttgcgc tcatctgccg tcataagatg aaagtcagag gtgataaggt
    18061 cctcgctact gatccactct gtggcacatg cattgccagt cataaaaaat aagaccatac
    18121 agatgaccaa tatattaatt ctattcataa tcttcaatga tatttatatc aaccttgatg
    18181 aattatgaat atatcggaaa ttgaaaaaat cgcagcaaag gaaatattat tatggggtta
    18241 tgagaggaca tttcagaaaa atgccctccc gttcaagcgg aaggcgagga attacagata
    18301 gcggcacaga taagaggtgg gttcggcaac ttgcagatga aactcactgt gaccgggaac
    18361 attaaacacc gaaccggctt catacacttg ccagtcggtc gcgtcaggca gtaacacatt
    18421 cagcgcgcca ctgattaccg tcatctcttc cggctcagcg gtgctgaagg tgtattcgcc
    18481 ttcaaccata acacccacgc tggcgcgacc agtgctgctg ctggaaaagc cgatcgattt
    18541 cactttgccg gaaaagtact cattactttg aagcataaac tggccctgtc tggtagttga
    18601 atatctactt cactataggg gccagatgcg ctggctgtca cgcaaaatta caccagtaat
    18661 tccgcagcca gccgtgcaat caatacgtta gagagcaaga caggaacatc gagctgtttt
    18721 tgcagtaaat cgcgatgacg ctggttaaat cccaaacaat ccagcatgat gacatctgcg
    18781 cctttcgcca gtaattcttt cccggcatca atgatttttt gttctgaatc atgaatgggg
    18841 ttacccaatg aaaatacagg cggtttctgc aaaatttgcc atttttgcgc ctgaacggtc
    18901 agcaactcct caaccggtac gataaccccc acctgatgat cttcaacaat agaggaaacc
    18961 agcggtggca atatcctcga cggctcaaga aagatcgtat tacgcgcagt catactacta
    19021 atgtttgccg tactcattaa tataatgacg tcgtagccct gattatcgag cacttcaacc
    19081 acaccttgca ggtcacgctc tactttgcga cgcgaaacat gggccagatg gttgtcattt
    19141 aataatgtga gaattgtgtc ttcgcctgct tctggcgcgt actccgccat cacttcttcg
    19201 cggcttaact tccccagcag gctatgatgg gaaatattat cttcgtcaat gtattctgtc
    19261 aggagcggca aaacttcctg cataggtaca atgccgatgg ttagaatcgc caacgacgca
    19321 ctcattgttc ttccacctta cttttatttc gtcgttaatt accgggcaag tgttaagcac
    19381 cactgctgac acttacttgt gctacaaatg acaaaaagcg tagcagcaga cgtgcacggc
    19441 ataatgtaaa gattccaaat gattccagta atggattgtt attgtttaat attctaatta
    19501 tttgaaaaac atgaattatg aaaaatgtga cgcagatccc ttgtagtggc actcagaatc
    19561 ccttccgagc agtctgatgc ggtcactctt tgtcagggtc ctcatagcgt tttttcgcat
    19621 ccatcgcttc ctgagcggtc ggatgttcac tgatcaacga gtctggttta ggatgatcgg
    19681 ctctgagttg gtaccaggtt accgtctgtc ctggctttcc tttttcaatt gtcactatat
    19741 atgcttcacg aggataaggc ggtttcgttg gcatcgttct tccttatttc acgggatgaa
    19801 cgttaagtat aggcgctcga aaatcaacaa ttgatcgtct gcgccagggc gctgcgaatt
    19861 tcagaaatca cctggctggg ttcgtttgtt gcgtcgatga taatatgcgc aacttcgcga
    19921 tatagcgcat cgcgttcttc cagcacttcc tgaacttctt cgctcagcgg ttttcccgtt
    19981 aaggttggcc gtaaatcttc ttccggtgca gcttgcagtc ggttaaccag gactgatact
    20041 ggcgcacaca aataaaccac gatcccgtta ttttgcatga agtgacgatt aaattccgtc
    20101 agaataatgc cgccgcctgt agcgataacg gtggatggcg cagttaccgc ttccagcgcc
    20161 gccgtttctc tggcgcgaaa tcccgcccac tcttcccttt cgacgatctc cgcgaccgtc
    20221 atattgagct gtgattgcaa ccactgatcg gtatcgacaa accgacggtt aagcgaatcg
    20281 gcaagggcca ttccgaccgt tgttttacca cagccccgag gcccgatcag aaaaagaggt
    20341 tgtgtcatcg tgggttttcc ccaataggtc gcaatgcggc gaaagccggt gtcatgagaa
    20401 tagcgatcat accatcaaac tagtacaatt tcgattgtaa agaaaaaatt ccacttaaag
    20461 tgaaaatctc aatacatccc ttactatacc aataaatatt caagaatgaa gtgtaaataa
    20521 taaattacat ttagccacga ctacgttgca cttccagcca ccacttctcc agctccgcgg
    20581 caaaggcctg gcggtcacgt tgtgaaaggc tatctggtcc gccggtctgg atcccactgg
    20641 cacgtaaggt atccataaaa tcgcgcatcg tcaggcgctc acgaatggtc gctggcgtgt
    20701 aacgttcacc gcgcggatta agcgccgcag cgcctttctc gatggcttca gcagccaaag
    20761 gtatatctgc ggtgatcacc aaatctcccg cttcacactg ccggacaatt tcgttatcgg
    20821 caacgtcgaa acctgccgcg acgcgcagcg tacgaataaa tcgcgatggc ggcacgcgta
    20881 aactctggtt tgctaccagt accagcggca tctgcatacg ttccgccgcg cgatacaaaa
    20941 tctctttaat tacattggga cacgcgtcgg catccaccca aattgtcata aagtcatcct
    21001 ttgttgggta atcctctatt gtgtcgcgct tttgccttcc ggcatagttc tgtttatgct
    21061 tctgccagcg attatcaaaa caatgaattt cacggcagga gtgaggcaat ggaaaagaaa
    21121 atcggtttta ttggctgcgg caatatggga aaagccattc tcggcggtct gattgccagc
    21181 ggtcaggtgc ttccagggca aatctgggta tataccccct ccccggataa agtcgccgcc
    21241 ctgcatgaca agttcggcat caacgccgca gaatcggcgc aagaagtggc gcaaatcgcc
    21301 gacatcattt ttgccgccgt taaacctggc atcatgatta aagtgcttag cgaaatcacc
    21361 tccagcctca ataaagactc tctggtcgtt tctattgctg caggtgtcac gctcgaccag
    21421 cttgcccgcg cgctgggcca tgaccggaaa attatccgcg ccatgccgaa cactcccgcg
    21481 ctggttaatg ccgggatgac ctccgtaacg ccaaacgcgc tggtaacccc agaagatacc
    21541 gctgatgtgc tgaatatttt ccgctgcttt ggcgaagcgg aagtaattgc tgagccgatg
    21601 atccacccgg tggtcggtgt gagcggttct tcgccagcct acgtctttat gtttatcgaa
    21661 gcgatggccg acgccgccgt gctgggcggg atgccacgcg cccaggcgta taaatttgcc
    21721 gctcaggcgg taatgggttc cgcaaaaatg gtgctggaaa cgggagaaca tccgggggca
    21781 ctgaaagata tggtctgctc accgggaggc accaccattg aagcggtacg cgtactggaa
    21841 gagaaaggct tccgtgccgc agtgatcgaa gcgatgacga agtgtatgga aaaatcagaa
    21901 aaactcagca aatcctgatg actttcgccg gacgtcaggc cgccacttcg gtgcggttac
    21961 gtccggcttt ctttgctttg tagagcgcca aatctgccga tttcaaccac tcgcgatagt
    22021 gactcatttg tgggttcagc ggcgcaaccc ccacactaat ccgtaaattt acctgtggcg
    22081 tattcggcaa acgtaatgta tttagccctt catgcacccg taacatggcg gtaatggcgc
    22141 tctcagctgg cgtaccggac atgattaccg caaactcatc gccgccaaac cgaccaatca
    22201 catcgctacc gcgcagggta atttgtaact gtcgggtaag cgccacaatc gcttcatcgc
    22261 ccacatcatg gccccaggta tcgttgatgc tcttgaaatg gtcgatatcg ataatcagta
    22321 acgttgcatc gcgattatgc cgccgacagt tatcaaattc attgcgtaac atagtttccc
    22381 aatgacgtcg gttatacacg cccgtcatgc cgtcgcgggt actcatgacc tgcaacctgc
    22441 gtttatgttc cgccagtttg gttgccgtct ggtagctgac ccagccaaac agcagaggat
    22501 aaatgacaat aatgggaagg gagagccacc attccagcgg cgcactattg aacgacacgg
    22561 taatgcccgt cagctcgagg gtgacaaggc aggaaaccac catcaacacc agacccgcga
    22621 caaacagacg ggggccgcct gcccccatca aattcagaca cataatcatc aacatcgcgg
    22681 tggaaggcag cacgtttacg cccattacgc ctacccacat tcccgctaat actgcatcgg
    22741 tttttaagtt gtaaatttcc cggctaagcg gatcgacggc cctgctcgct atctgccagg
    22801 ctaaatgcgg ccagacgaac gcccagccga ccaacaccag ccaccaccag cccggcggcg
    22861 ggtgtgaaac cagcgttgaa gcaatcggta agaacatgcc agccaggcca accgcacggg
    22921 gtagtctgac gcgacgggcg aagcgcagcc cggaccgctg atgttcgttt tgaggagtta
    22981 aaggaggttc ctccccgtgc gccgccgctt ttttgaaaaa gttttcatca ttcattattt
    23041 ttgggaacat tcagaaacaa ttttcccaaa ttatagagac ggggctaaag gcagaagtat
    23101 gaaatttcgg gtgagccatt ttagtagctc acccgttagc actatcaggt atcaggcggc
    23161 tttcttgagg caggcgctca taaacttatt acgatcatca cctttcagtg attgttgcgt
    23221 tgcttgatta ttacattcgc gcatcttttg ctgctgtggc gtcaaacttt tttcaccagg
    23281 cgcagacttg ctgttcttca ggcaatcact catgtaggtc ttacgagcat cccctttcaa
    23341 cgcctgtgcc gtcgcctgct gattacagga ggtcatacgc tgttgttgtg gggttaaggt
    23401 tctctcggca gcgccgacgg tggttaaaaa aaccagaccg aaaagcaagg taaccagtaa
    23461 tgtaattttc atagcaccat ccctcttcat gttttaacca tgagcgtatg cgcccgtgat
    23521 ctgacattaa gtctggttgc taacagcaaa aaaaccaccc ggcagcgaaa attcactgcc
    23581 gggagcggtt ttatttcagc cccagagcgg ctttcatggt gtagaagaga tcggtctggt
    23641 cggtcagtcc aacgacattg gcggcatgcg ggccatacgc cgcaatacgc aactgactgc
    23701 cggtatgttc ttgtgaatcc tcttcggagt tcccgtaact catcaccatc actgcgccat
    23761 ctttggtatt tagcgcctga gtgaggcccg gagctttggt atccggcgca acaatctggc
    23821 tggcgtgggc gtgatcagcg gtgactatga ccagcgtgtt accatccttt ttggcgaatt
    23881 ccagcgcccg ttgtacggct tcatcgagat cgaccgtctc gccaatttgc ccacaaggat
    23941 tcgcagcatg atcctgttta tcgattgacg caccttcaac ttgcaggaaa aagcctttct
    24001 catttttact caacaattca atggctttgt cggtcatctg tgccagggtc ggtacactgt
    24061 cattacgttg cggattaggc gtacaggtga ctgcgggctt atcgatatta ccgtggtacg
    24121 ttgctttcgg tcctagccag cgcactggca tattgccgtc agcaaacagg ccaagcaggg
    24181 gtttttgctg attcgcttcc gtcaccgaat tcagtgaggc agcatcgctc accaactgat
    24241 aaccacgcgc ctgtgcctgt tcacgcagcg tttttccctg ccattcacca gcggttgccg
    24301 tttcagcaaa ggtttttgcg ccgccgccaa gcgtaacgtc ggcacgagcg ttaagcagct
    24361 gttcggtaat cgatcctttt ccgccttttt ccagagcgtt acccggacat ttttcactgg
    24421 tcgcgctcgg accgtagcat ttgcgcgagg tcacatgcgc caccagcgcg gcgggcgtgg
    24481 catcctgcaa ctctgcggta gaaacgttac cggtcgccag acctgcggct tttgccattt
    24541 ccagaatcgt tgggtgatct ttttcgtgaa tatcgacgcc cagcgcgccg ttataggttt
    24601 tgacaccggt tgaccaggcg gttgctgatg cagccgagtc ggtgacgtag tccggttttc
    24661 cggttttttt attcagcgca tagtgagtgt attgcccggt aagcggtaag gcatcgatac
    24721 ctttaaaaaa gccgcccgca ccttcggcat aattacgtgc ggcagtaatt tccgaatccc
    24781 ccatcccatc gccaatcagc aaaataatat tttttgcagg tttatcgcta agagaatcac
    24841 gcagagcggc ggtctgatca gccgttaagc ggcgagcacc gccgggtgca gtaatatcgc
    24901 cctgagcagc ccggttttcc agaacaggca tttctggtgt ccgggctttt gtcacagggg
    24961 taaacagtaa cggtaagagt gccagtgcaa tagtgctttg tttcacttta ttttctccat
    25021 gtacaaatac attaaaaaat aaaaacaaag cgactataag tctcggccgt gacaacttta
    25081 tgacagctgt tgaaaagatt aactttttac tgacgaggat gcttcaataa cttctttacg
    25141 taatcgcgca gcagctccgt atcgtcgtca ggaatgctgg catcgggctt tacctcgtac
    25201 agcgccccct ctacctgatc aatcaaccgc tgttggtcat tttgcgccat attgcgaagc
    25261 attgcagtga cgataatctc caaagcttct acctgcgcac acagttcttt cgactcttct
    25321 tctttttggg caagcttaaa taacaactca gcaatgagat ttttcatgtc tgtatttcct
    25381 tatccaaagt atggagaagt taacattatg tttcacaact tcatagtgtc tggaggtatt
    25441 atttttatgg caaaggaaat actttgcgca aatatttttt gtttgattac cagcgaaacg
    25501 tttctcttat ttaaata
//
LOCUS       DAZKIF010000067        24819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZKIF010000067 DAZKIF010000000
VERSION     DAZKIF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.067"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            66..806
                     /gene="yiaT"
                     /locus_tag="ACHOTW_002682"
     CDS             66..806
                     /gene="yiaT"
                     /locus_tag="ACHOTW_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFP9844761.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(936..2906)
                     /locus_tag="ACHOTW_002683"
     CDS             complement(936..2906)
                     /locus_tag="ACHOTW_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFP9844762.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(2917..4317)
                     /locus_tag="ACHOTW_002684"
     CDS             complement(2917..4317)
                     /locus_tag="ACHOTW_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9844763.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            4543..5358
                     /locus_tag="ACHOTW_002685"
     CDS             4543..5358
                     /locus_tag="ACHOTW_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9844764.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            5360..5500
                     /locus_tag="ACHOTW_002686"
                     /pseudo
     CDS             5360..5500
                     /locus_tag="ACHOTW_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            5602..6075
                     /gene="ysaA"
                     /locus_tag="ACHOTW_002687"
     CDS             5602..6075
                     /gene="ysaA"
                     /locus_tag="ACHOTW_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9844765.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(6227..7480)
                     /gene="avtA"
                     /locus_tag="ACHOTW_002688"
     CDS             complement(6227..7480)
                     /gene="avtA"
                     /locus_tag="ACHOTW_002688"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP9844766.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(7658..9688)
                     /gene="malS"
                     /locus_tag="ACHOTW_002689"
     CDS             complement(7658..9688)
                     /gene="malS"
                     /locus_tag="ACHOTW_002689"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9844767.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            10008..10832
                     /gene="bax"
                     /locus_tag="ACHOTW_002690"
     CDS             10008..10832
                     /gene="bax"
                     /locus_tag="ACHOTW_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP9844768.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(10940..12118)
                     /gene="xylR"
                     /locus_tag="ACHOTW_002691"
     CDS             complement(10940..12118)
                     /gene="xylR"
                     /locus_tag="ACHOTW_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP9844769.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(12196..13377)
                     /gene="xylH"
                     /locus_tag="ACHOTW_002692"
     CDS             complement(12196..13377)
                     /gene="xylH"
                     /locus_tag="ACHOTW_002692"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP9844770.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(13355..14896)
                     /gene="xylG"
                     /locus_tag="ACHOTW_002693"
     CDS             complement(13355..14896)
                     /gene="xylG"
                     /locus_tag="ACHOTW_002693"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP9844771.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(14974..15966)
                     /gene="xylF"
                     /locus_tag="ACHOTW_002694"
     CDS             complement(14974..15966)
                     /gene="xylF"
                     /locus_tag="ACHOTW_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9844772.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            16332..17654
                     /gene="xylA"
                     /locus_tag="ACHOTW_002695"
     CDS             16332..17654
                     /gene="xylA"
                     /locus_tag="ACHOTW_002695"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP9844773.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            17726..19180
                     /gene="xylB"
                     /locus_tag="ACHOTW_002696"
     CDS             17726..19180
                     /gene="xylB"
                     /locus_tag="ACHOTW_002696"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9844774.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            19349..19690
                     /gene="yiaB"
                     /locus_tag="ACHOTW_002697"
     CDS             19349..19690
                     /gene="yiaB"
                     /locus_tag="ACHOTW_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP9844775.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            19736..20173
                     /gene="yiaA"
                     /locus_tag="ACHOTW_002698"
     CDS             19736..20173
                     /gene="yiaA"
                     /locus_tag="ACHOTW_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP9844776.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(20215..21210)
                     /gene="wecH"
                     /locus_tag="ACHOTW_002699"
     CDS             complement(20215..21210)
                     /gene="wecH"
                     /locus_tag="ACHOTW_002699"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP9844777.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            21385..21684
                     /gene="ysaB"
                     /locus_tag="ACHOTW_002700"
     CDS             21385..21684
                     /gene="ysaB"
                     /locus_tag="ACHOTW_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP9844778.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            21779..22690
                     /gene="glyQ"
                     /locus_tag="ACHOTW_002701"
     CDS             21779..22690
                     /gene="glyQ"
                     /locus_tag="ACHOTW_002701"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9844779.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            22700..24769
                     /gene="glyS"
                     /locus_tag="ACHOTW_002702"
     CDS             22700..24769
                     /gene="glyS"
                     /locus_tag="ACHOTW_002702"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9844780.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         6124 a         6114 c         6419 g         6162 t
ORIGIN      
        1 cggagaaatt gtaagcatta ctatgccgac tcttttttat cttattttaa aatggaatac
       61 cggacatgtt aattaaccgc aatattgtgg cgttatttgc gttgcctttt atggcaagcg
      121 caactgcttc tgaattatcc attggtgctg gtgcggctta taatgaatcg ccttatcgcg
      181 gttataatga aaatacaaag gcaattccgc tgattagtta tgaaggtgat tctttttatg
      241 ttcgtcagac cacgttaggt tttattctgt cgcaaagtga aaaaaatgaa cttagcctga
      301 ccgcatcctg gatgccgctg gaatttgacc ctgccgataa tgacgattat gccatgcaac
      361 agcttgataa gcgtgatagt acggctatgg cgggggttgc ctggtatcac cacgagcgtt
      421 ggggaaccgt gaaagcctct gcagctgcgg acgttctgga taacagcaac ggctgggtgg
      481 gggagctatc ggtattccac aaaatgcaga taggtcgtct gtcgctgaca cctgcgctgg
      541 gcgttctcta ttatgacgag aatttcagtg actattacta tggcatttca gagagtgagt
      601 cccgtcgtag cggtctggca agttattccg cgcaggatgc ctgggtgccc tatgtcagcc
      661 tgacggcaaa atacccgata ggagagcacg tcgtattgat ggcgagcgca ggatacagcg
      721 agctgccgga agagattacc gacagcccga tgattgatcg taatgagagt tttacctttg
      781 tcaccggcgt gagctggcgt ttttaattca ccggtggatg tcggtgcggc tggcctacgc
      841 tctcgcaccg acttgtatgc ctgataagac gtgttaagcg tcgcatcagg caatgtgctc
      901 cttacgttgg atgcggcgcg agtgccttat ccaacctacg gatggcaatg cttttcctca
      961 ttcacccaga tccgcatttc gccttcaccc cggttggccc agctaaacca cgggataaag
     1021 gtcagagttt gcgtctggcg tttggctggc gcgctgtcgt aatgccacag cggttgctgc
     1081 tctggattgc tctgttcata ccggtaaccc ggtgcctgga ttaagatctt atggctaaac
     1141 aatcccttgc cttcaaatgt cgtaaatggc gcatcggtgg gcagccacag attatgcagt
     1201 gactcgccgt tgtcggcctt ttccaggcaa tacaccagcg ggccgcgctg aatcgccact
     1261 tttccggcga cgtgacgcac cagcgggtta ccgtaaacgc ggcgtaccgg catcggcaaa
     1321 gtcagattca gcgtatcgcc ctcctgccat tcgcgggtaa tgtgcaaata ccctttacga
     1381 atatcctgct cgacctcttc cccattcaat atgatctgcg gctgtgtgca ccagtccggc
     1441 agacgtaaag ccagcgtatg acgtaccggc tggggcgatt caaccgcaat cgtcacctgc
     1501 tcctgccacg gatagttccc gctaacccgc aggcgcagcg tgccattttc taccggcact
     1561 tccatgctgt ttcctgcgta gatgttgata tacaacgcat cttcacgcgg cgtgtagaga
     1621 taatgaccaa tcgaggtcag cacgcgggcg atatttggcg gacaacaagc gcagccaaac
     1681 caacgctggc ggatcggttt aacgtgatcg tagatatggt tgaatttcag cgattttgga
     1741 tgtacttcca gtggattcac atagaagaaa tgtttgccat ccaatgccat gccgccgagc
     1801 acagtgttgt acagtgcgcg ctccatcaca tcggcatatt gactgtcgcc ttccatttcc
     1861 agcattcgtc gggcgaacat catcaggccg atggaagcac aactttcggc gtaaaccgtg
     1921 tcattcggca gatcgtaatc gctgctgaac gcttcgccgc tgctttgtga gccgatgccg
     1981 ccggtaatat ataactgacg ctgggccata ttgttccaca gccgcaggca gtcctgacgc
     2041 ttgctgtcat cgtgacttaa acgcgcgaga tgcgcgacgc cggtcatcag atagacaaaa
     2101 cgtaccgcgt gaccgatggc ggtttgctgt tgcgcaaggg gcaaatgtgc ctggctgtag
     2161 gctttgtctt tcaccatcca cgccgggccg taggtgtgcc agtgcgatgt ctgcccgcgc
     2221 ttttcatatt cctggtcgta atagtgcggt tgcgcaccac gctgttcgac aaaatagttc
     2281 gtcagcgcca ggtagcgcgg ctcttcagtc acttcataca ggcgcattag tgccagttca
     2341 atttccgggt gaccaggata accgtgtaac ttactttcat ctggaccaaa tacgctgtcg
     2401 atatgatcgg ccaggcggca aacgaccccc agcaagcgcc gttttcccgt ggcctggaag
     2461 aaggcgactc cggcttcaat cagatgacct gcgcagtaaa gttcatgaca ctccgccaga
     2521 ttgctccagc gttcttcggg tgcttttacc gtaaagtaag tattgagata gccgtcttcg
     2581 cattgggcgg aggcgatcag ttcgattacc tcgtcggcgg ttttttccag ttcggcgtcc
     2641 ggcttctggc acagcgacca ggctaccgct tccagccatt tggcgacgtc gctgtcctga
     2701 aacaccatcc cgtaaaattc accctcctga agtccggcgg caatgcgaaa gttttcaatc
     2761 gcatggctgg gttccgcttc tgggatacga tcgttcaagg catcccactg ataaggaatc
     2821 accacgtcgc ggaccagttg ttggtactga ccgaggaacg gatcgctgac cgtcagttta
     2881 tgcagatcga cttccgaaat gttcatcttg tgctccttaa ggctgtacat gacgctgacg
     2941 caggtcggta gctatgcgtg acatcatagg attattgagt ttgcaaaagc ggatcgcccc
     3001 tgccagcagc aggtggaata gcgcaggcag taatgtttcc atcgccgcga tcccgccaag
     3061 cgaactggcg gtctggtttt ccacgccagg ctggtaggcg acaaaaataa acagcaggct
     3121 gatgatcccg gcgctggagg cccaggccag cttgatgaaa aacagattga aggcgaagtt
     3181 catgccggaa gaacgtacgt gggttttcca ctcgccgtag tcatcggcaa aggccatcaa
     3241 tgagaagtgc agtggcaatg tgaagccaag aatcacgcca ttaccgagga tcaccgccag
     3301 ccacagcgtt tgataagccg ggccggaggg caggaaccac atcaacaccg ccagtgcagc
     3361 gaggagcagg ttggtgtagt aataaatttt gacggtatcg aaacgccgcg ttaacgggct
     3421 gacaatcacc gagccgataa tggaggcgaa ggtgaccatg gtgaagaaca gcgacgtgta
     3481 gcccgtgctg ccttgtaaga cgtaggtaat gaagtacata tacccaccgc cgcgaatgtt
     3541 aaagacgtta atcagcagga aagacatgac cagcatcagc agcagttggt cgttgttccg
     3601 cagcccggca agatgttcgc gcagggtaaa tttccccatt gtggagagcg gcacccgttc
     3661 acgaacccag aagaaacagc acaggaacat caccacggca atggcgcaca ataccccgac
     3721 gcccagttga tagccgcgtg cagcgttgcc ctgaccgaag agagctacca tccacggtaa
     3781 gcctaccgaa accagaaatc ccgctacgcc gcagagaacg aatcgccagg actggcagga
     3841 gatcacttca ttgtggcggg tggtcatggt gttgatccgc gcgcagtacg gtacgttgat
     3901 ggcggtataa ccgactgaaa ggagcaggta agtaccgaac gcccaggcga tttttactcc
     3961 catgctggca tctggcacgg tgaacgtcag gatgccgata atgccgatgg gaatggcaat
     4021 ccatagctgc cacggacgaa agcgccccca tcggctttgc gtccggtcgg caatgacgcc
     4081 catcaccggg tcggagatgg catcaaacac gcgcagtgca ataaacagcg tgccaaccag
     4141 cgccggagtt aatccaaaaa cgtcggtgta gaaaaaggtg agaaaattca tgatcaggca
     4201 agttattacg gtgccgcctg cgtcacccag gccgtagcct attttttccc gtacagataa
     4261 cgcatcgtca attttacctt tagcgatatc agcggtagta atcggtgtag aggtcataga
     4321 tattcctcgt gaatagggag caatttttag ttttattgaa atgacctgat taattctgcc
     4381 gtgaacggga atatcaacaa ggcgagaaaa gagtaaaaaa aggtgacgat tccgacctga
     4441 atttaaaagt gtgaaatgga tcaggtgaaa ttaatgtttt tgattaataa tcaaaaggta
     4501 atgtgtttaa tcattaatga ataagcggga aattacatat caatgctcga attatccatg
     4561 acgttaccga ttaaagtgca aaatggtgga ttatttattt cccgtggcgt agggaggcat
     4621 tctgcaaggc ggctggagtc atgggaaatt atctttgtcg aaaaaggtcg attaacgatt
     4681 caggaagaag aacggatatt tctggtcgat gcgggagaga gtttattact ctggccgaac
     4741 cgacaacatg ttggggtgga ggattttccc gccgatctca aattttactg gctgcatttt
     4801 gaggttaaag cgccggacaa cgaccctcgc tggcttactc atcttagcgt gccgcaacaa
     4861 acgaaggttg tcgatccgca ggcgctggtg gccctgtttc gccagttctt aagcgaacag
     4921 gaaaagcatc aacgcactcc ggcgctggag tttattgtgc tgctcatttt gcagcagctc
     4981 acccgtgacg cgcgtcttga tgaacaggcc gatggcgcag gcaccaccct tgcctggaaa
     5041 gcgcagaaac tgatccgcac gcaatatcat cgcccgcttt ctacggcgct tctggcccgc
     5101 gagctgtact gcaatgccga ttatcttggg cgcgtctacc ggcgggtgtt tcatctgacg
     5161 ttaacagaag caatccatcg ccagcgggtg cgcgaagccg aaaagctgtt aattagcgaa
     5221 gcgcgatcgc tcacggaagt ggcggcgttg tgcgggttta atgacgttgg ctatttccgg
     5281 cagatttttc gcaaacacac cggtttaacg ccagcagcgt ggaagcggcg ttacagcaag
     5341 gaacatatca attcgtagtg ccggggcgat ttcgctttcg acatcacgtc tgaaacaggt
     5401 gggggagaaa aatctcctga aaccactgcg tgaaaccgcg caggctattt ctaatgaact
     5461 gggatttact gtcagggacg accagggcgc gatcacataa cgctttggac aagtgccaaa
     5521 actttaacat ttccttcgtt ggatcaaagc agtagggacg cgctctctgg cactctgctg
     5581 ttttagtgca aaggagtgat catgaaccgg tttattattg cggatgcgac gaaatgtatc
     5641 ggttgccgta cctgtgaagt ggcttgcgca gtgtcgcatc aggagaatca ggattgcgct
     5701 gcgttgtcac cagacgagtt tatttcccgt attcgtgtca ttaaagacca cagctggacc
     5761 acggcagtag cctgtcatca gtgtgaagat gcaccgtgcg cgaatgtctg ccctgttgac
     5821 gcgataagcc gcgaacatgg gcatattttc gttgaacaaa cacgttgcat tggctgtaaa
     5881 agctgtatgc tggcttgccc gtttggtgcg atggaggtcg tttcttcgcg caaaaaggcg
     5941 agggcgatta agtgcgatct gtgctggcat cgggagacgg gaccggcctg tgttgaagcc
     6001 tgcccgacaa aggcgttgca gtgcatggat gtcgagaaag tgcagcggca tcggctacgg
     6061 cagcagcctg tttgaaacgt ttttccggcg tttctcaacg ccggaatagt gcgctttgtt
     6121 tatgccagat gcggcgtaaa cgccttatcc ggcccacaaa ttcttgcaaa ttaaatatat
     6181 tgcagaaatc atgtaggcct gataagcgta gcgcatcagg caatttttag tgactttcag
     6241 cccaggctct ttctatctct tccgccagaa tcttcacccc cgcctcaatt ttctccggct
     6301 ctggtacgta gttcatgcgc atacattgat gcgtatgcgg ccacggtttg tccagccctg
     6361 ggaagaagtt gtgccccggc accatcagca cgccgcgtgc tttcaggcgc tgatagagct
     6421 gctcggtcgt aatgggcaaa tccttaaacc aaagccagag gaaaatggct ccttctggtt
     6481 tatgaattag gcagcgatct tccggtaaat agcggcgaat gatggcgata gtttcctgaa
     6541 cacgctggta gtaaaacggt ttgatgactg tttcagacag gcgcagcaga tcgttacgct
     6601 taatcatttc acacatcatc gccggaccaa taccgccagg tgccaggctg ataatgccgt
     6661 tcatattggt gatggcggtg atgatttttt cattggcgat gataatgccg cagcgggagc
     6721 caggcagccc cagcttggaa agactcatgc acagcacgat attcggattc catagcgggc
     6781 gcgcttcact gaagatgata cccgggaacg ggacgccata agcgttatca atcaccagcg
     6841 gaatgccgtg ttgattcgcc agcgcgtcaa gcttcagcaa ctcttcgtca gtaatcacat
     6901 tgcctgttgg attcgtcggc cgggagacgc aaatcatccc ggtttcttcg ccaatatgca
     6961 gatgctcaaa atcgacgtgg tatttaaact ggccttccgg cagcagttca atattcggac
     7021 gcgcagagac aaacagatct tcttccagtc cggcgtcagc gtagccaatg tattccggtg
     7081 ccagcgggaa cagcactttt ttgacccgac catcggcacg gcgtccggca aacagattaa
     7141 ataagtagaa aaacgcgctc tggctgccgt ttgttagtgc aatattctgt gcttcgatat
     7201 cccaacccaa cttctcgcgc agcattccgg caagcagtgt gagtagctcc gttttcccct
     7261 gtggaccgtc gtaattgcac agtgcatcag tcgctttgcc actttccagc atgtcggtca
     7321 gtagcgtctg gaagtagtcc tgcatttccg ggatctgcgc cggattaccg ccgccgagca
     7381 taatcgcgcc aggcgtgcgt aaaccgtcgt tcagatcttc catcagcagc gtaatgccgg
     7441 agtggcgggt aaatttgtca ccaaaaaggg agaatgtcat agcgggatat ctgtcgaact
     7501 ctgtagcaag gaaggtaaca ataacgctac atttgctggg gtgcaaatcg gtctgttgaa
     7561 gagtgagcgg tgctttatcc tgcaacgctg attagggctg acattttatc ctggttggcg
     7621 gcttcccgag gtactgacga acgggaagcc ggaaaagtca ctgttgccca gcccagatga
     7681 ccagcacttt atcgtcgcca tgctcacgaa caaagccgta gccctgcttc agcgaaagtg
     7741 tcgtttgttt gcccgcgcca attgcgggat ggcgggcgcg gaactggctg attttctgcc
     7801 agtgcgcgac gttggcggca gatttaccgc taacatcctg ccagttcata tccgaacgtg
     7861 taccttgcag cggatcagaa cctgtaggac cgaacggacg cgaggattca tcgccataaa
     7921 agatttgtac cgcgcctggc gctaatagta ataactctgc tgctttgtcg cccccttcac
     7981 ggaacagacg ggtatcatgc gacgagaggt agctcaacac gttgaaaccc tgcaatttct
     8041 ccgccatttg ctgccaggtc gtatccatct gcgcaataca atcgacagct ttcgccgcct
     8101 gctcctgata atcgaaattg atcatcgcat cgaagccgtg gcgatagtag tcactttgca
     8161 tcacgccgtg gccccaggct tcaccggtca tccagaaagg tttgtcatct aatgctttgt
     8221 cggggttagc ttttttccat tcgcgaagcg cggcgctggc ttcggttttc agttgctgcc
     8281 aagcgggcaa ctcaacatgt ttggcggtat cgacccgaaa accatcaatc ccatagtcgc
     8341 ggacccactg acttaaccag tgggttaagt aatcgcgagg ggtaaagccg tcgatggctt
     8401 tagcgtgggt atccgttttg tttttataga acaccggcag accagaagcg gtagttgatt
     8461 cggttttgat atccggcaaa aaggctagcg acatggtgag atcgtcgaat ccaggattgt
     8521 cgtaatcgcc gatatcggta cggatccagt tttttcccca ccatttatcc cagcctgttt
     8581 tgtcgctgaa attaatgtaa tcgttaaagc tatgccaggt ttgcccggcg gcaggtttcc
     8641 agtcgctcca gcgttcaccc agcgtttttt tcacttcgtc accagaaaga tataacgcgc
     8701 caaactgata ctcctgcata tccgccagcg tggcatagcc ggtgtggttc atcacgacat
     8761 caaagagaat acgaataccg cgctgatgtg cgctatcaac cagcgtccgt agatcggctt
     8821 cgttgcccat attggcatca agattcgtcc agtcctgtgt gtaataaccg tggtaggcat
     8881 aatgcgggaa atcgcctttt gtaccgccgc cgacccagcc gtgaatttgc tcaaatgggg
     8941 cgcttatcca taaagcatta acgcccaact gctggaggta atccagtttg ttggtcaggc
     9001 cgcgtaaatc gccgccgtga aaagtgccaa tttccgccat accgtcttta tgacgtccgt
     9061 aactctggtc attactggga tcgccgtttt cgaaacgatc tgtcagcaca aagtaaaccg
     9121 tggcgttatg ccagtcgaaa ggggcagagg cgtcagtttc tgcccgttcc agcaggagta
     9181 aaccgttgct ggtagcagcg ggttgtaacg ttatttgacc gttcttcact atcgcaattt
     9241 gctggctgta ataatcccgt acggcggctc cttccgggaa agtggcgctg acatccagcg
     9301 tgagcggtaa tccatcccat ttcgggcatt cacggaccag gtttgctacc ggttcggcgg
     9361 cgttctggat ggaaatcatc aatgttggcg taccggagcg ggtgtctatt tgcagcgtat
     9421 atttgccgtc cctgaacaat cgccattgag gcggcgtgtt gctacaaggt tgcagggaaa
     9481 gcatctgatt gagttttatc gcatctgcag gctgccagca ctgctggtca aaatttagcg
     9541 tgagtggacg cgtacctttg ggcaactgcg cgtggctgac aaatgttccc gttccctgtt
     9601 cgctaaaggc agggaacccc ggagaagtcc agctggcggc aacggcgaag ccaggaagga
     9661 gtgtcagaaa acaggcggcg agtttcatgg tggatgagtc cttattgctg cgatttcaga
     9721 cagtttgcca gcgataagcc agacaaaact catccctgag cggtaagtcc acaggatgag
     9781 aagcaagggt gagcgatcgc gcgcaaaaaa cggctgaatt ttgcgataaa ccccacattt
     9841 tctgcgattt agcgccaatc tgaatcgtta actcgtgaca tagtttcaga tttggactat
     9901 tccttggggt gctcttgaca gctatttttg atatgatttg agattcaact ctcaaatttg
     9961 tgaaaaatat aaggtgttgg aatgattaaa tccgaccagg agacctgatg attttgactc
    10021 ccatacgacg atatggggcg atgattctta tgttactcac tctggtgttt tcgagtgagg
    10081 tgttagcgaa gacgcacaca acaacagcga gtcaaaagtc ccacttaact aaagctagta
    10141 ataaacaggt aagcagtaaa caagagtatt ctcgcaatag tgcaaagagt agttcacttc
    10201 ctgatttgcg aaaataccct tccggaacat caaggaaaaa ggcgtttctc cggaccgtaa
    10261 tgccttacat taccagccaa aatgcagcca ttactgcaga acgtaactgg ctgatttcaa
    10321 aacagtatca gggccaatgg tcacctgctg agcgtgcgcg tctgaaagac atcgccaaac
    10381 gctacaaggt gaagtggtct ggtaatacgc gaaaaatccc gtggaatacc ttgcttgaac
    10441 gcgtagacat tatccctacc agtatggtgg cgacgatggc tgcagcagaa agcggttggg
    10501 gaacgtcgaa gctggcgcgc aacaacaaca atcttttcgg catgaaatgc atgaaaggac
    10561 gttgtaccaa tgcgccaggt aaagtgaaag ggtactcaca gtttagttct gtcaaagaat
    10621 cggtgagcgc ctatgtcact aacctcaata cgcatccggc ttactcttcg ttccgtaaat
    10681 cgcgtgcgca gctgcgtaaa gctgatcagg aagtgactgc cacagcgatg attcacaagc
    10741 tgaagggcta ctcgaccaaa gggaagagtt ataacaacta cctgttcgca atgtaccagg
    10801 ataaccaacg gttaatcgcg gcgcatatgt aattgtattt cctcgagcct tatccgactt
    10861 gtcggtcgga taaggctttt gatcgcatca ggtggttgtg ctgagttacc tgatgcgacg
    10921 ccgacaattc tcatcatcgc tacaacatga cctcgctatt tacatcgcga tactcttttg
    10981 gcgtcgtgtc atatgctttt ttaaaaacag agtagaaata ttgcagcgat ggataaccgc
    11041 acatttgcga tatctcattg atcgacaagg tggttgaaat cagcagactg cgcgctttct
    11101 ccagcttctc agcatgaatc atggcatgga tggtttcacc cacctcttct ttaaaacgct
    11161 tctcaagatt ggagcgcgag atcccgaccg catcgagtac ctgatccact ttaatccctt
    11221 tacaggcgtg attacgaatg taatgcatgg cctgaataac ggcgggatcg gtcagcgaac
    11281 ggtaatctgt tgagcgccgt tcaatgacgc gaactggagg gaccaaaatc cgctgtagcg
    11341 gcatttcttc tttatctaat aatcgatgca acagttttgc cgcctgatag cccatttgcc
    11401 gcgcgccctg agcgaccgaa gaaagggcga cacgcgacag atagcgggtc agttcttcgt
    11461 tatcgatgcc aatcacgcat aatttttccg gtacgggaat gtgtagatgt tcacatactt
    11521 gcagaatatg ccgcgcccgg gcgtcagtaa cggcaataat cccggtttgc ggtggcagtg
    11581 tttgtagcca gtctgccagc cgattttgcg cgtgttgcca gttctctggt gcggtttcta
    11641 acccctgata aaccactccg cgatactttt cttcggcgac aagctgacga aatgcatatt
    11701 cgcgttcagt ggcccaacgt ttgccgcttg attccggaag accataaaaa gcaaagcggt
    11761 taacgccttt ctcttttaaa tgcaaaaatg cgctttcaac cagcgcatag ttatcggtgg
    11821 caatgtaatg aacgggtggg taactttctg caaggtgata cgagccgcca accccaacaa
    11881 tggggacgtc gacatcagcc agcgcttgct cgatctgttt gtcgtcgaag tcggcaatga
    11941 cgccatctcc taaccagtcc ttgattttat caatgcgggc gcggaaatct tcttcaatga
    12001 aaatatccca ttccgattgt gacgcctgta aatattcccc tacgccttct actacctgcc
    12061 ggtcataggc tttattggca ttgaacagta atgtgatgcg gtgacgttta gtaaacatgg
    12121 ttcttttcct gctgaatcat gcaaaaactc aaaaccggta atacgttacc ggctttgaga
    12181 aaatttttat caaaatcaag aacggcgttt ggttgcggag tccatccata ctgccagcaa
    12241 cagaatcgca cctttaacga tatactgcca gaaggtcggt acatccatca tactcatacc
    12301 gttatccagt gaagccatga tgaatgcccc cattaccgct ccagccacgc tcccgacgcc
    12361 gcctgccaga ctggtgccgc caatcacgca tgctgcaatt gcgtccagct cggcgatatt
    12421 ccccgcagaa ggtgaaccag cgccaagtcg agaactgagg attaatccgg cgatggctac
    12481 cattaatccg ttaatagcga acacggcaag tttggtgcgt tcaacgttaa ttccggagag
    12541 acgtgctgct tccagattgc cgccgatggc ataaatgcgt cgtccaaatg ctgtccgcgt
    12601 tgccataaac attccgccga gtaacagcaa cgtcagcagc agaacaggag taggaacgcc
    12661 acggtaatca ttcaacagcc agattgcgcc taatacgatg atagcggtta aagcctggcg
    12721 accgactact gcggtagagg ctggagactg caaacccaaa gcctgacggc gcattcttcc
    12781 gcgccattgc cagccaacaa aagccattaa gccaagcgcg ccaatgatga agccggtgct
    12841 ggcggggaga tagctttgcc caatttgtga catcgcggcg ctggtggggg atacagtcgt
    12901 gccgttggtg atgccaatga gtatgccgcg aaatgccaac atgcccgcga gagtgacaat
    12961 aaatgaaggg actttgcggt acgcgaccca ccatccgttc caggcaccga gaagcagtcc
    13021 cagaaccaac gtcacaatga tggtaagtgg caaaggccag cctagccaga cgtcacaaat
    13081 cgccgcgacg ccacctaata gccccatcat tgagccgacg gaaaggtcga tttcagcaga
    13141 aattatgacg aacaccattc ctaccgcgag gatgccggta atcgcggtct ggcgtaacag
    13201 gttggagacg ttacgggcgc ttaagtaggc accatcggtg gtccaggtaa agaacagcat
    13261 gattgcgatg atagctgcaa tcatcacgaa gacctgcaaa ttcagtgatt tcagcccgga
    13321 gaagccaccg gatgtcggta cggccaattt cacttcagac ggattgcttt tcgacatgat
    13381 gttcgctcct caatgcggct tccatcacct gctcctgagt caggttatga tttatcaggt
    13441 tggcttttag tttcccttca tgcattacta gtacacgatc gctaaggccg agcacttcag
    13501 gtaattcaga agagatgaca ataacggcaa taccctgctg gacgagttgg ttaattaatt
    13561 tgtagatctc gtatttcgcg ccaatatcga tacccctggt gggttcatca agaatgagaa
    13621 tgcgcgggtt aagtaacaga cagcgagcga ggatcgcttt ttgctgattg ccgccgctca
    13681 aacgtccaat agcaaggtcg ggggacgacg ttttaacttt gagttgctgg attgattcca
    13741 gaatacattt ttgctctgcc gcgtcatcaa gctggctaat gccaccggta aatttattga
    13801 gtgcggcgag ggtaatattt ttaccaaccg ccattaccgg aacgatgccg tcgcgctttc
    13861 tgtcttcggg gaccatcgca atcccctggg cgatggcttg ctgacagtta cgaatatcta
    13921 cctgtttgcc atcaatataa atttttcctt cccattgtcc gggccacaca ccaaacaggc
    13981 actgaatggt ctcggtacgt ccggcaccaa cgagtccggc aatacccagt atttcgccac
    14041 gtttcaggga aaacgagaca tcattaactc gtttaatatg acgattaacc ggatgccatg
    14101 ccgtcagatg ttcaatacgt aatatttcat ctccggtggt atgtggttca ttagggtaaa
    14161 gcgcggttaa ctctcgcccg accatcatgg tgataatatc gtcttcactc attccggcag
    14221 catcacgcgt accaatgtgc tgtccgtcgc gaataacgca aatcgtatcg gaaatcgctt
    14281 tgacttcgtt gagtttgtgc gaaatataaa tacaggcgat accgtgctgt tgtagatcgc
    14341 gaataatatc cagtaaaacc gacgtttcct gctcagttaa tgaggctgtc ggttcatcga
    14401 gaattaacaa gcgcacctgt ttattaagtg ccttggcaat ttcaaccagt tgttgttgcc
    14461 caagccctaa atcgccaacg cgggtatcag gtgaaatgga taaactgacc tgtgcgagca
    14521 gcttctgaca gcgtagcgtc atcaggtcat aatccataat gccattgtgg gttatttcgt
    14581 tacccaggaa gatattttcc agcacggtca attctttcac cagggccaat tcctggtgaa
    14641 tgatggcgat acctttgcgt tcggtatcgc ggatgtgact cgcctgaatc tcttctcccg
    14701 caaaaataat ttcgccttcg taggagccat ggggataaat accacacagc actttcatca
    14761 gcgttgattt accagaccca ttttccccac agagtgagac gatttcgcca gcattcaacc
    14821 gcaagctgac gttatcaatc gccttcacac tgccgaaggt tttggtaatg ttcttcattt
    14881 caagtagata aggcatagcg actccaccta agccaattca ttcacgcggc atggagagaa
    14941 atcacgcccc cgctccgcgc tggggcgtaa cgcttacagc tcgctctctt tgtggaatcc
    15001 gtctttaatt accgtatctt tgatgttgtt tttattcaca tcgatcggtg tcaggaggcg
    15061 ggaggggaca tctttcaggc cattatttag tgaagtatct gcttttggct cctgaccatt
    15121 gcccaattca acggcaattt ctgcggcagt atttgccagc aatgtaatag gtttatatac
    15181 cgtcatagtt tgcgtaccgg cagcaatacg tttaatacct gcgagatccg catcctggcc
    15241 agaaattgct actttccctg ataaaccttg cgcgcttaat gcctgaattg ccccacctgc
    15301 ggtggcatcg tttgaggcaa ctacagcatc aattttgtta ttattggcgg ttagcgcgtt
    15361 ttccataatt ttcaatgcgt tttccggtaa ccagccatca acccattggt caccaacgac
    15421 tttaattttt ccggaatcaa cgtaaggttg taacactttc atttgtccgg cgcggaacag
    15481 cttagcgttg ttatctaccg gcgagccgcc catcaggaag taattacctt gcggaacaat
    15541 atcgaccagg gcttttgcct gcagttcacc gactttttcg ttatcgaaag aaatataaaa
    15601 atcgatatcc gcatcgttaa tcatacggtc gtaagctaat actttaatgc cttcttgttt
    15661 ggcttctttt acaacgttac ttaatacctg accgttatac ggaataatga caagaacatc
    15721 gacaccccgg tttatcatgt tttcaatctg cgacatttgt gtttcttcat tgccatttgc
    15781 agactgtaca aatactttcg cgccgagaga ttctgccttt ttcacaaaga tatctcgatc
    15841 tttttgccag cgttcaagac ggagatcatc aatcgccata cctattttga cttctttggc
    15901 gtgtgcagca acgttggtaa gcaggagtga ggtgcaaagg gtgagtagaa tgttctttat
    15961 tttcatggtg tagggccttc tgtagttaga ggacagcttt aataagtaac aatcaccgcg
    16021 ataaacgtaa ccaattttta gcaactaaac aggggaaaac aattacagat ttttatcttt
    16081 cgattacgat ttttggttta tttcttgatt tatgaccgag atcttacttt tgttgcgcaa
    16141 ttgtacttat tgcatttttc tcttcgagga attacccagt ttcatcattc cattttattt
    16201 tgcgagcgag cgcacacttg tgaattatct caatagcagt gtgaaataac ataattgagc
    16261 aactgaaagg gagtgcccaa tattacgaca tcatccatca cccgcggcat tacctgatta
    16321 tggagttcaa tatgcaagcc tattttgacc agctcgatcg cgttcgttat gaaggctcaa
    16381 aatcctcaaa cccgttagca ttccgtcact acaatcccga cgaactggtg ttgggcaagc
    16441 gtatggaaga gcacttgcga tttgccgcct gctactggca caccttctgc tggaacgggg
    16501 cggatatgtt tggtgtgggg gcgtttaatc gtccgtggca gcagcctggt gaggcactgg
    16561 cgttggcgaa gcgtaaagca gatgtcgcat ttgagttttt ccacaagtta catgtgccat
    16621 tttattgctt ccacgatgtg gatgtttctc ctgagggcgc gtcgttaaaa gagtacatca
    16681 ataattttgc gcaaatggtt gatgttctgg caggcaagca agaagagagc ggcgtgaagc
    16741 tgctgtgggg aaccgctaac tgctttacaa accctcgcta tggcgcgggt gcggcgacga
    16801 acccagatcc agaagtcttc agctgggcgg caacgcaagt tgttacagcg atggaagcaa
    16861 cccataaatt gggtggtgaa aactatgtcc tgtggggcgg tcgtgaaggt tacgaaacgc
    16921 tgttaaatac cgatttgcgt caggagcgtg aacaactggg ccgctttatg caaatggtgg
    16981 ttgagcataa acataaaatc ggcttccagg gcacgttgct tatcgaaccg aaaccgcaag
    17041 aaccgactaa acatcaatat gattacgatg ctgcgacagt ctatggcttc ctgaaacagt
    17101 ttggtctgga aaaagagatt aaactgaaca ttgaagctaa ccacgcgacg ctggcaggtc
    17161 actctttcca tcatgaaata gccaccgcca ttgcgcttgg cctgttcggt tctgtcgacg
    17221 ccaaccgtgg cgatgcgcaa ctgggctggg acaccgacca gttcccgaac agtgtggaag
    17281 agaatgcgct ggtgatgtat gaaattctca aagcaggcgg tttcaccacc ggtggtctga
    17341 acttcgatgc caaagtacgt cgtcaaagta ctgataaata tgatctgttt tacggtcata
    17401 tcggcgcgat ggatacgatg gcactggcgc tgaaaattgc agcgtgcatg attgaagatg
    17461 gcgagctgga taaacgcatc gcgcagcgtt attccggctg gaatagcgaa ttgggccagc
    17521 aaatcctgaa aggccaaatg tcactggcag atttagccaa atatgctcag gaacataatt
    17581 tgtctccggt gcatcagagt ggtcgccagg agcaactgga aaatctggta aatcattatc
    17641 tgttcgacaa ataacggcta actgtgcagt ccgttggccc ggttatcggt agcgataccg
    17701 ggcatttttt taaggaacga tcgatatgta tatcgggata gatcttggca cctcgggcgt
    17761 aaaagttatt ttgctcaacg agcagggtga ggtggttgct tcgcaaacgg aaaagctgac
    17821 cgtttcgcgc ccgcatccac tctggtcgga acaagacccg gaacagtggt ggcaggcaac
    17881 tgatcgcgca atgaaagctc tgggcgatca gcattctctg caggacgtta aagcattggg
    17941 tattgccggc cagatgcacg gagcaacctt actggatgct caacaacggg tattgcgccc
    18001 tgccattttg tggaacgacg ggcgctgtgc gcaagagtgc gctttgctgg aagcgcgagt
    18061 tccgctatcg cgggtgatta ccggcaacct gatgatgccc ggatttactg cgcctaaatt
    18121 gctatgggtt cagcggcatg agccggagat attccgtcaa atcgacaaag tattattacc
    18181 gaaagattac ttgcgtctgc gtatgacggg ggagtttgcc agcgatatgt ctgacgcagc
    18241 tggcaccatg tggctggatg tcgcaaagcg tgactggagt gacgtcatgc tgcaggcttg
    18301 cgacttatct cgtgaccaga tgcccgcatt atacgaaggc agcgaaatta ctggtgcttt
    18361 gttacctgaa gttgcgaaag cgtggggtat ggcgacggtg ccagttgtcg caggcggtgg
    18421 cgacaatgca gctggtgcag ttggtgtggg aatggttgat gctaatcagg caatgttatc
    18481 gctggggacg tcgggggtct attttgctgt cagcgaaggg ttcttaagca agccagaaag
    18541 cgccgtacac agcttttgcc atgcgctacc gcagcgttgg catttaatgt ctgtaatgct
    18601 gagtgcagcg tcgtgtctgg attgggccgc gaaattaacc ggcctgagca atgtcccagc
    18661 tttaatcgct gcagctcaac aggctgatga aagtgccgag ccagtttggt ttctgcctta
    18721 tctttccggc gagcgtacac cacacaataa tccccaggcg aaaggggttt tctttggttt
    18781 gactcatcaa catggcccca atgaactggc gcgagcagtg ctggaaggcg tgggttatgc
    18841 gctggcagat ggcatggatg tcgtgcatgc ctgcggcatt aaaccgcaaa gtgttacgtt
    18901 gattggtggc ggggcgcgta gtgagtactg gcgtcagatg ctggcggata tcagcggtca
    18961 gcagctcgat taccgtacgg ggggggatgt ggggccagca ctgggcgcag caaggctggc
    19021 gcagattgcg gcgaatccag agaaatcgct cattgagttg ttgccgcaac taccgttaga
    19081 acagtcgcat ctaccagatg cgcagcgtta tgccgcttat cagccacgac gtgaaacatt
    19141 ccgtcgcctc tatcagcaac ttctgccatt aatggcgtaa acgttatccc ctgcctgacc
    19201 gggtggggga taattcacat ctatatatct cagcaattaa ttaatattta gtatgaattt
    19261 attctgaaaa tcatttgtta atggcatttt tcagttttgt ctttcgttgg ttactcgtaa
    19321 tgtatcgctg gtagatatgg agatcgttat gaaaacctca aaaactgtgg caaaactatt
    19381 atttgttgtc ggggcgctgg tttatctggt tgggctatgg atctcatgcc cattgttgag
    19441 tggaaaaggc tattttcttg gcgtgttaat gacagcaact tttggcaact atgcatatct
    19501 tcgcgcagaa aaactcgggc aactggataa tttttttacc catatttgcc agttagttgc
    19561 gttaatcact atcggtctct tgtttatcgg tgttttaaat gcacctatca atgcttatga
    19621 aatggtgatc taccccatcg ccttttttgt ctgcttgttt ggtcaaatgc gtttgtttcg
    19681 ctcggtatga gcaacataaa gctcttacac attcaggaat gaaaggaata ctgtgatgga
    19741 caacaaaata tcaacctatt caccggcctt tagtattgtg tcatggatag ctctcgttgg
    19801 tggtatcgtt acctatctgt tagggctatg gaatgcagag atgcagttaa atgaaaaagg
    19861 atattatttt gccgtactgg tattaggact gttttctgcg gcgtcttatc aaaagaccgt
    19921 tcgggacaag tatgaaggca taccgaccac ttccatttat tatatgacct gcctgactgt
    19981 ctttattatc tctgttgcat tactgatggt aggtctgtgg aatgcgacat tattactcag
    20041 cgaaaaaggt ttttatggac tggctttctt cttaagcttg tttggtgcag tagcggtgca
    20101 gaaaaatatt cgtgatgccg gaataaaccc accaaaagaa acacaggtta cccaggaaga
    20161 atacagcgaa taactcacgt aagcccggtc agtccaatgt gaccgggctt ttacttaact
    20221 cactaatctg tttctgtcga ttcgttgtac cagcatagaa agtaacaaac tcgctgccaa
    20281 cgtcgcgcaa aaaatccaaa taatatccag tattggccaa tttttaagct caattccccg
    20341 ggtgcgcagc gcatggataa tcaaggcgtg gaatccgtat atacccaacg aatggcggga
    20401 gattaagcca agtccgcgaa tggtacgcgt atccagcgtg tttttaacca gagtcaatag
    20461 cgcgattgcg cagataaaaa ccatcggccc acagtaagga taccaggtat cggcaaaatt
    20521 tccgcgccac tgcaattcat ataatgtccc gcgagagata ataaaaactc ccgtcgcaaa
    20581 cagtgcggcg ctcacccacg acagtgcttt atgctgtgtg tccatcatcc ctaaagcgcg
    20641 gcccaacatg ccatacagaa tgtagtaaaa agtatcgcca ttgatatata agttaattgg
    20701 cagccattca aaaccgtcga ttttctgcgg cacggtgttt gggttagcga tgataccaat
    20761 caccaccatt agcaccagca acatttttcc gccgacgttc ttcacctgaa tcagcggtga
    20821 aaccagataa atcaccgcaa tcgcgaagaa aaaccataag tggtaaaaca ctggcttttg
    20881 cagcaggttt ttcagcgcta actccacatt aatggaggta aacagtgcaa tgtagagcag
    20941 cgcgattgcg ctataaaaaa acagacataa gccgatacgc aagaaatggc gcggctgggc
    21001 gctgcgttcg ccaaaaaaga gatagccgga aatcatgaaa aatagcggca cgctgacacg
    21061 agaggcagag ttcagaacat tggcgatatc ccaggtgacg gggctaacac tatgagcatt
    21121 ggtcacatac caggtagtgg tgtgaatcat caccaccatt aaacacgcta tccctcgcag
    21181 gttatcaatc cagtaaattt tgggctgcat ctgtgtctct gtatctggtt aaaaaaagtc
    21241 tgaccgataa atcattcagt tggcgcactg gaataatctg agttttaaca ctacagctta
    21301 tagaggctta aggaaattcg taagatatca gccactatac cgatataaat aataagactc
    21361 acctgcaaac cagacggtaa tttaatgatg atgaactctt tctttccggc aatggcgctt
    21421 atggtgctag tgggttgttc tacaccgtca cccgtgcaga aagcacaacg ggtaaaggtt
    21481 gatcctctgc gttcgttgaa tatggaagcg ttatgcaagg atcaggcggc aaaacgttat
    21541 aacaccggcg agcaaaaaat cgacgtcacc gccttcgaac agttccaggg aagctatgaa
    21601 atgcgcggtt ataccttccg taaagagcag tttgtctgtt cttttgacgc agatggccat
    21661 tttttgcatc tttccatgcg ttaagccctg ctttttcccg tttcatactg tatatcttcc
    21721 atccagcggg tatactgatc ccttccttta aatccacacg tatccagcac gaaataatat
    21781 gcaaaagttt gataccagga ccttccaggg cttgatcctg accttacagg attactgggc
    21841 tcgccagggc tgcaccattg ttcaaccatt ggacatggaa gtcggcgcgg gaacctctca
    21901 cccaatgacc tgtctgcgcg cgctggggcc agaaccgatg gcggctgctt atgttcagcc
    21961 ttctcgtcgc ccgaccgatg gtcgctacgg cgaaaacccc aaccgtttac agcactacta
    22021 tcagttccag gtggtcatta agccatcgcc ggacaatatt caggagctgt accttggttc
    22081 tctgaaagag ctgggcatgg acccgactat ccacgatatc cgtttcgtgg aagataactg
    22141 ggaaaacccg acgctgggtg cctggggact gggctgggaa gtgtggctaa acggcatgga
    22201 agtgacgcag ttcacttact tccagcaggt tggtggtctg gagtgtaaac cggttaccgg
    22261 cgagatcacc tacggtctgg aacgtctggc catgtacatt cagggcgtag acagcgttta
    22321 cgacctggtc tggagcgacg gcccgctggg taaaaccacc tacggcgacg tgttccatca
    22381 gaacgaagtg gagcagtcca cttacaactt cgaatacgcg gatgtggact tcctgttcac
    22441 ctgctttgag cagtacgaga aagaagcgca gcagctgctg gcgctggaaa atccgctgcc
    22501 gctgccagct tacgagcgta ttctgaaagc cgcccacagc ttcaacctgc tggatgcgcg
    22561 taaagccatc tccgtcaccg agcgtcagcg ctacattctg cgcattcgca ccctgaccaa
    22621 agcagtggca gaagcatatt acgcttcccg tgaagcccta ggcttcccga tgtgcaacaa
    22681 agataagtaa gaggcggcta tgtctgagaa aacttttctg gtggaaatcg gcactgaaga
    22741 gctgccacca aaagcactgc gcagcctggc agagtccttt gctgcgaact ttactgcgga
    22801 gctggataac gcaggcctcg cacacggcac cgttcaatgg tttgctgctc cgcgtcgtct
    22861 ggcgctgaaa gtagctaacc tggcggaagc gcaaccggat cgtgaaatcg aaaaacgcgg
    22921 cccggcgatt gcccaggcgt tcgacgctga aggcaaaccg agcaaagcgg cagaaggttg
    22981 ggcgcgtggt tgcggtatta ccgttgacca ggctgagcgt ctgactaccg ataaaggcga
    23041 atggctgctg tatcgcgccc atgtgaaggg cgaaagcact gaagcactgc tgccgaatat
    23101 ggttgcgact tcgctggcga aactgccgat tccgaaactg atgcgctggg gtgcaagcga
    23161 cgtgcacttc gtgcgtccgg tgcacaccgt gaccctgctg ctgggcgaca aagtcattcc
    23221 tgcaaccatt ctgggcattc agtccgatcg cgtgattcgc ggccaccgct ttatgggcga
    23281 gccggaattc accatcgata acgccgatca gtatccggaa attctgcgtg agcgtgggaa
    23341 agtcatcgcc gattacgaag aacgtaaggc gaagattaaa gccgatgccg aagaagcagc
    23401 gcgtaagatt ggcggtaacg ctgacttaag cgaaagcctg ctggaagaag tggcttcgct
    23461 ggtggagtgg ccggtcgttc taaccgcgaa atttgaagag aaattcctcg cggtgccgtc
    23521 tgaagcactg gtttacacca tgaaaggtga ccagaagtac ttcccggtgt atgcgaacga
    23581 cggcaaactg ctgccgaact ttatcttcgt tgccaacatc gaatcgaaag atccgcagca
    23641 aattatctct ggtaacgaga aagtcgttcg tccacgtctg gctgatgccg agttcttctt
    23701 caacaccgac cgtaaaaaac gtctggaaga taacctgccg cgcctgcaaa ccgtgctgtt
    23761 ccagcaacag ctgggtacgc tgcgtgacaa gactgaccgc atccaggcgc tggctggctg
    23821 gatcgccgaa cagattggcg ctgacgttaa ccacgcaacc cgtgcgggcc tgctgtctaa
    23881 gtgtgacctg atgaccaaca tggtcttcga gttcaccgac acccaaggcg ttatggggat
    23941 gcactacgcg cgtcacgatg gcgaagcgga agacgtagcc gtcgcgctga atgagcagta
    24001 tcagccgcgc tttgccggtg atgacctgcc gtccaacccg gtagcttgtg cgctggcgat
    24061 tgctgataag atggatactc tggcgggtat cttcggtatc ggtcagcatc cgaaaggcga
    24121 caaagacccg tttgcgctgc gtcgtgccgc acttggcgtg ctgcgaatta tcgttgagaa
    24181 gaacctcaac cttgatctgc aaacgctgac cgaagaagcg gtgcgtctgt atggcgataa
    24241 gctgactaat gccaacgtag ttgatgatgt tatcgacttt atgctcggtc gcttccgcgc
    24301 ctggtatcag gacgaaggtt ataccgttga caccatccag gcggtactgg cgcgtcgtcc
    24361 gactcgtccg gctgatttcg atgcccgcat gaaagcggta tcgcacttcc gtaccctgga
    24421 agcagctgca gcactggcgg cggcgaacaa acgtgtatct aacatcctgg cgaaatctga
    24481 cgaagtgctg agcgatcgcg tgaatgcctc tactctgaaa gagccggaag aaattaaact
    24541 ggcgatgcag gttgtggtgc tacgtgacaa gctggagccg tactttgcgg aaggtcgtta
    24601 ccaggatgcg ctggtcgaac tggcagagct gcgtgaacca gtcgatgcct tcttcgataa
    24661 agtgatggtc atggttgatg acaaagaatt gcgtatcaac cgtctgacca tgctggagaa
    24721 actgcgcgaa ctgttcctgc gcgttgcgga tatttcgctg ttgcagtaat aacgccgtta
    24781 ttaaaaagcc tgccatctgg caggcttttt ttatcgcta
//
LOCUS       DAZKIF010000068        24773 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZKIF010000068 DAZKIF010000000
VERSION     DAZKIF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.068"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..537
                     /locus_tag="ACHOTW_002703"
     CDS             <1..537
                     /locus_tag="ACHOTW_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFP9844781.1"
                     /translation="QTYDEQVNASWSEVLSQYQRRADLIPNLVASIKGYSSHERDVLE
                     AVTLARSQANRASSDLQQTPGDEQKLQAWQQAQAQVTRTLGQLTIISERYPELKSQEL
                     YQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKFPAVLTAKVMDYTPKKNYLPDDV
                     AAVSKAPTIDFSQNANAH"
     gene            551..1225
                     /locus_tag="ACHOTW_002704"
     CDS             551..1225
                     /locus_tag="ACHOTW_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9844782.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            1459..2607
                     /locus_tag="ACHOTW_002705"
     CDS             1459..2607
                     /locus_tag="ACHOTW_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9844783.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            2604..3518
                     /locus_tag="ACHOTW_002706"
     CDS             2604..3518
                     /locus_tag="ACHOTW_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9844784.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(3578..4876)
                     /gene="eno"
                     /locus_tag="ACHOTW_002707"
     CDS             complement(3578..4876)
                     /gene="eno"
                     /locus_tag="ACHOTW_002707"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9844785.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4964..6601)
                     /gene="pyrG"
                     /locus_tag="ACHOTW_002708"
     CDS             complement(4964..6601)
                     /gene="pyrG"
                     /locus_tag="ACHOTW_002708"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP9844786.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6829..7620)
                     /gene="mazG"
                     /locus_tag="ACHOTW_002709"
     CDS             complement(6829..7620)
                     /gene="mazG"
                     /locus_tag="ACHOTW_002709"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP9844787.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7691..8026)
                     /gene="mazF"
                     /locus_tag="ACHOTW_002710"
     CDS             complement(7691..8026)
                     /gene="mazF"
                     /locus_tag="ACHOTW_002710"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFP9844788.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(8026..8274)
                     /gene="mazE"
                     /locus_tag="ACHOTW_002711"
     CDS             complement(8026..8274)
                     /gene="mazE"
                     /locus_tag="ACHOTW_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFP9844789.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(8352..10586)
                     /gene="relA"
                     /locus_tag="ACHOTW_002712"
     CDS             complement(8352..10586)
                     /gene="relA"
                     /locus_tag="ACHOTW_002712"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP9844790.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10634..11935)
                     /gene="rlmD"
                     /locus_tag="ACHOTW_002713"
     CDS             complement(10634..11935)
                     /gene="rlmD"
                     /locus_tag="ACHOTW_002713"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9844791.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            11992..14748
                     /gene="barA"
                     /locus_tag="ACHOTW_002714"
     CDS             11992..14748
                     /gene="barA"
                     /locus_tag="ACHOTW_002714"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP9844792.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(14981..16321)
                     /gene="gudD"
                     /locus_tag="ACHOTW_002715"
     CDS             complement(14981..16321)
                     /gene="gudD"
                     /locus_tag="ACHOTW_002715"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP9844793.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(16342..17682)
                     /gene="gudX"
                     /locus_tag="ACHOTW_002716"
                     /pseudo
     CDS             complement(16342..17682)
                     /gene="gudX"
                     /locus_tag="ACHOTW_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(17684..19036)
                     /gene="gudP"
                     /locus_tag="ACHOTW_002717"
     CDS             complement(17684..19036)
                     /gene="gudP"
                     /locus_tag="ACHOTW_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP9844794.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(19471..19920)
                     /gene="yqcA"
                     /locus_tag="ACHOTW_002718"
     CDS             complement(19471..19920)
                     /gene="yqcA"
                     /locus_tag="ACHOTW_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9844795.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(19938..20720)
                     /gene="truC"
                     /locus_tag="ACHOTW_002719"
     CDS             complement(19938..20720)
                     /gene="truC"
                     /locus_tag="ACHOTW_002719"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP9844796.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(20720..21049)
                     /gene="yqcC"
                     /locus_tag="ACHOTW_002720"
     CDS             complement(20720..21049)
                     /gene="yqcC"
                     /locus_tag="ACHOTW_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP9844797.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21671..22216)
                     /gene="syd"
                     /locus_tag="ACHOTW_002721"
     CDS             complement(21671..22216)
                     /gene="syd"
                     /locus_tag="ACHOTW_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP9844798.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            22284..23132
                     /gene="queF"
                     /locus_tag="ACHOTW_002722"
     CDS             22284..23132
                     /gene="queF"
                     /locus_tag="ACHOTW_002722"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP9844799.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            23244..24608
                     /gene="ppnN"
                     /locus_tag="ACHOTW_002723"
     CDS             23244..24608
                     /gene="ppnN"
                     /locus_tag="ACHOTW_002723"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP9844800.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         6096 a         6504 c         5946 g         6227 t
ORIGIN      
        1 caaacctatg atgagcaggt taacgcctcc tggtctgagg tattgagtca atatcaacgt
       61 cgcgccgatc ttattcctaa tcttgtcgcc agcattaaag gttattccag ccatgaacga
      121 gatgtactgg aagccgtaac attggctcgt agccaggcta atcgcgccag tagcgatctt
      181 cagcaaactc cgggagatga acaaaaatta caagcctggc aacaggctca agcgcaggtg
      241 actcgcactc tcggtcagtt aacgataatt agtgagcgtt accctgagtt aaaatctcag
      301 gaactctatc aaaatttaat ggtacagctt gaaggaagtg aaaatcggat tgccgttgcg
      361 cgaggaagat atattaaagc cattgaacaa tataatgtca ctatccgcaa attccccgct
      421 gtattaaccg caaaggtcat ggattacaca ccaaagaaaa attatttgcc ggatgatgtc
      481 gccgcagtaa gtaaagcccc aacaatagat tttagccaga atgctaatgc tcattaagcg
      541 gggaagttct atgcgaaatt ttatcttcct tatggctttc ttctgttcat ctgtgtttgc
      601 cacacaaatt ccagtacctg aatcacccaa gtatgtgaat gacttaaccg gcacattaac
      661 aaacagcgaa gtaaacaccc tgaccaacca gattaaagcc ctgacgcaaa aaaactacgc
      721 acagttggtc gtgttagtgg ttgaaacaac aggcgatgaa accatcgagc agtacgcgac
      781 gcgggtattc gatagctgga aaccgggtga taaagaccgc gatgatggag ttctactgtt
      841 agtcgcctgg caggatcata ccgtgcgtat tgaaatcggt tacggactgg aaggtattat
      901 tacggatgcg cagtccggga aaattattcg caacagtatt attcctgcat ttaaaaaagg
      961 cgacctggct ggtggtttgc aaaagggaat cagcgatatt gaaagtcgtc tgacggggaa
     1021 taatttagca acgataacac cgaccgatca ccctttgccc tttagcggtt ggtgggcatt
     1081 gttagtctgg gcgatagtac tgacgtttat ttctgccaga ggatatatca aaacgctggg
     1141 agtaatttgc ttcgcagcta ttgtactggc ctttgtttta cctatagcag gcttcagcgg
     1201 aagctgggga gtactcgcaa catgactctg cttcgccaca ccgttcctgg ctgtcgccat
     1261 cgcttttacc cccttcggca aaaaagttcg ggatttgatg cgtgatgcca accagccatc
     1321 acgccataca agaagcaatt cttcctccca ctattcatca tcgtcatcct cttcattcga
     1381 caatgacaat tttagcggtg gtggcggttc atctggtggc ggtggcgctt ccgggcgctg
     1441 gtaacaaaag gaattgatat gcgtattttt ctcttacttc tgatgctatg cggttttcag
     1501 gtattcgctg aacgattgcc ttcagtgact gacttaaccg ggacattaac gacagaagaa
     1561 caaactgctt taactcaaca attacagact ctcgagcagc aaaaccactt ccaggtggcc
     1621 gtgttggtta cgccgaccac agggggcaaa aacattaaac ttgcagcctc gcagagatac
     1681 ggggtatatc actggaaacc cgttggagag aagcgatatg gcgaaggtat cttaattctc
     1741 gtcgtatggc cggaagggct ggcgtcaatg aagattggac atggactgga agagatgttg
     1801 ccgccggaac aagctgcgca aattgttcgt taccatatgc agccagaatt cgaaaaaaac
     1861 aatctctttg ctggattaac gggcggaatt gaaagcatcg cacagtttac ccatatcaaa
     1921 gcgaacctta gccctttgga tgctttagcc aatcatctgt ttgctaatcc gcaacgttcg
     1981 ctgccatgtc ttgcctggac actgctgatg atcgttgcca tcgttgtcct ctggctattt
     2041 gccagccgcc cacggcgtgg catatggatg atatcgatga ttacccctat ggtctggttt
     2101 ttctcctttc aggacgatgt catcattcgg cgggtatcgg tcgtttgttt tagtttattg
     2161 ttagcaacaa cctgcaagca gcgtctcgcg gtagttttta atatgtgccg cgtatttcct
     2221 atgatgctga ccccaaaaaa taaaaacgct aaagtgaaaa aacaaaagaa gactccgcag
     2281 gttcgtgaat caggacgaaa ttcattgttt gtcgtgatgc tgataattat tgtgggatgg
     2341 tgcctggtgt tcgccagtaa taaagatatt gcattcatct caacaattat tgggttaatt
     2401 gatcatttta tcggcccaat aaccattgct ggcatcgttc tgctgatcat tgtggccaaa
     2461 tttactggca atcttaagct ggggtcagga gaaaaaagca acaggaaaac ggggaatcgt
     2521 aatgctcatt ctcgctcttc gtcgagaaat tctttccgcg gtggtggcgg ttcttcagga
     2581 ggcggcggtt catccgtccg ttggtgatcg taatgcgctt aatctttatt ctattcactt
     2641 tgtggtgcct tcctgggctt gcccagcaaa ttgctgtgcc ggaactgcgc caacaggtga
     2701 ccgatattac tggtacgtta agcacttctg aacaacaatc actgacacag caactgcagg
     2761 atattacaca taaaacacgg gcgcaggtcg ctgtattagt agtgccgtcc accggagacg
     2821 attccattga acaatatgct acaagggttt ttgatagctg gaaactggga gataagcaga
     2881 gaaacgatgg tattttactt ctggttgcct gggaggatca tgctgttcgt attgaggttg
     2941 gctacgggct ggaaggggtg gttaccgacc tgcaagcggc gaagattatc agggatatat
     3001 tgatccctgc cttcaaaagt gatgacctga tgggtggatt aacactggca agcgaaaata
     3061 tcggcgcgct tctgttgaat ggtgaattac cggaagacag gggggattat tacagtatca
     3121 atccccctat tccattatcg cttgctgtaa ttatattgct ggcggtactt tcatatttta
     3181 ttgtttttac cgatccgtcg aatttaccct ggatcactct cactggcgcc atttacggga
     3241 tggtattcct ctatgttgcc gagccaggac catggaccaa tttaatcgtt gcctgcggta
     3301 tgttaacgcc ttttgcgatc gtaccactgg ttattttttg gcttatcgtc aataaaaagc
     3361 tacgcgccaa atacaaaaaa ctcagtaaag atagagcttc aagaaaaggt tcttcttcat
     3421 cctcttccgg tggcggctca agtggcggag gctcaggcgg cggatttagc ggcgggggcg
     3481 gttcttctgg cggtggcggc gcgtccggtc gctggtaact caaccataaa aaacgccagc
     3541 ccgaaggctg gcatttttaa atcagataaa gtcagactta tgcctggcct ttgatctctt
     3601 tacgaccgtt gtacggtgct ttttcgccca gagcttcttc gatacgaatc agctggttgt
     3661 atttagcaac gcggtcagaa cggctcatag aaccagtttt gatctggcct gcagcagtac
     3721 caacagccag gtcagcgatg gtagcgtctt cagtttcgcc agaacggtga gagataactg
     3781 cagtgtagcc agcgtctttc gccatcttga ttgcagccag agtttcggtc agagaaccga
     3841 tctggttgaa tttgatcagg atggagttag cgataccttt ttcgatacct tctttcagga
     3901 tcttggtgtt ggttacgaac aggtcgtcac caaccagctg gattttgtcg cccagaactt
     3961 tggtctggta tgcgaaacca tcccagtcag attcgtccag accgtcttcg atggaaacga
     4021 tcgggtactg tttggtcagt tcttccagga agtgagtgaa ttcttcagag gtgaatgctt
     4081 tgttgccttc gccagccaga acgtatttac cgtctttgta gaattcagaa gctgcgcagt
     4141 ccatcgccag agtgatgtct ttgcccagtt cataaccagc agctttaaca gcttcagcga
     4201 taacagccag agcttcagcg ttggaaccca ggttcggcgc atagccacct tcgtcaccaa
     4261 cagcagtgtt catgcctttc gctttcagaa cttttgccag gtgatggaaa acttcagaac
     4321 ccatgcggat ggcttctttc acagttttcg cgccaaccgg ctgaatcatg aattcctgga
     4381 tatcaacgtt gttgtcagcg tgctcaccac cgttgatgat gttcatcatc ggaaccggca
     4441 tggagtattt gcccggagtg ccgttcagtt cagcgatgtg ctcgtacagc ggcatacctt
     4501 tagcagctgc agcagctttg gcgttagcca gagatacagc caggattgcg ttcgcgccga
     4561 atttggattt gttttcggtg ccgtccaggt cgatcatgat cttgtcaatg ccagcctgat
     4621 ctttagcatc tttgccaatc agcgcctgag cgatcgggcc gtttaccgca gcaacagctt
     4681 tggttacgcc tttacccagg aaacgggatt tgtcgccatc gcgcagttcc agagcttcac
     4741 gggaaccagt agaagcacct gacggagcag ctgccatacc gacgaaacca ccctccagat
     4801 gtacttcggc ttcaacagtc gggttaccac gggagtcgat gatttcacga ccgatgattt
     4861 ttacgatttt ggacattagg ttttcctcaa gtcactagtt aaactgaaac tccagacaaa
     4921 caacgcgtac ccagggtacg cgttgccgtt ctaacttttt tacttacttc gcctgacgtt
     4981 tctggaactc gctggcggct ttcacaaagc ctgcaaacag cgggtgacca tcacgtggag
     5041 tagaagtaaa ctccggatgg aactggcaag ccacgaacca cgggtgattc ggaacttcga
     5101 tgatctcgac caactgatca tccccggaac ggcccgcaac gcgcagacct gcatcttcaa
     5161 tctgtttcaa cagcatgttg ttgacttcgt aacggtgacg atgacgctca acaattgtcg
     5221 gcgcattgta cagctggcga accaggctat cgtcaaccaa ctggcactgc tgtgcgccga
     5281 gacgcatggt accgccgaga tcgctcttct cgctacgaac ttcaacgttg ccgttttcat
     5341 cgcgccactc ggtaatcagc gccacaaccg ggtacttaca gtctggcaca aattccgtag
     5401 agttggcgtt ctccatgttg gcaacatggc gagcgtaatc aattaacgcc acctgcatac
     5461 ccaggcaaat gcccagataa ggaatattgt tctcacgcgc aaaacgcgcg gtcgtaatca
     5521 tgccttctac gccacgatag ccgaaaccgc caggtacgag gattgcatcc agacctttaa
     5581 ggatttcaac gccgcgcgtt tcaacatctt gtgaatcgat cagtttgatg ttgacgctga
     5641 cacgattctt cagcccaccg tgtttcagtg cttcaatcac ggatttgtaa gcgtccggca
     5701 gttcaatgta cttgccgacc ataccaatgg tgacttcact taccggattc gcttcttcga
     5761 agataacctg ttcccattcg gacagatttg cttccgggca gtttaagctg aatcgtttac
     5821 aaatataatc gtccagcccc tgagatttca acaggcccgg aattttatag atggaatcga
     5881 cgtctttcag agaaataacc gctttttccg gaacattaca gaacaatgca atcttcgcac
     5941 gttcgttcgc cggaacggcg cgatctgaac gacaaatcag gatgtcaggc tggataccga
     6001 tggagagcag ctcttttaca gagtgctgag tcggtttggt tttgacttca ccagacgctg
     6061 ccatgtacgg caccagcgtc aggtgcataa acagagtgtg ctcacggcca atttcaacag
     6121 ccatctggcg aatcgcttcg aggaacggca gggattcgat atcacctact gtaccgccga
     6181 tttcgaccag tactacgtca tgaccttcgc caccttccag cacgcgctct ttgattgcgt
     6241 tagtgatgtg cggaataacc tgtacggttg cgccgaggta gtcaccgcgg cgttctttac
     6301 gcagaacgtc agagtagata cgacccgtgg tgaagttgtt gcggcggctc attttggtac
     6361 ggatgaaacg ctcgtagtgc cccaggtcca ggtcggtttc agcgccgtct tcagtaacga
     6421 acacttcccc gtgttggatt gggctcatag tacctggatc gacgttgatg tacggatcca
     6481 gtttcatgat ggtcacattg aggccacggg cttcaagaat ggctgcgagg gaggctgcgg
     6541 caatgccttt acccagagag gatacgaccc cgccggtcac aaaaatatag ttcgttgtca
     6601 tgctgaacct gagaagttag gttgaaaaga cgatggaata accaggacgg gaaagcagta
     6661 tacccgaaca tgacctgtgc cacaaacttt cattatccct cctcttcgcc agcgcactat
     6721 tgaaatcagg agtgagaaaa tagccccttt ggggtaaatg tttttgacgc aaatcaagcg
     6781 cttgtcattt aaaaaatgac acaaatggcg cttgaccgcg taattccctt agagatcaat
     6841 ttcctgccgt tttacctgtt gccagacttc ttccattgtt tcgaggtcaa cacctgtcat
     6901 ttccagtcca cgcgcggcaa caatacgctc cacttcgcga aaacgacgct cgaatttttc
     6961 gttcgctttt tgcaatgcga tttccgcttt tgtccctaaa tgacgagcca gattgaccgt
     7021 ggcaaacagc aggtccccca tttcctcctc cagtttagcc tggtcgacaa cagcctgccg
     7081 cgcttcgtac atcacctcgt cgatctcttc gtagacttta tcgactaccg gaccaagcgt
     7141 cgtccaatcg aagccaacgt tggcgcaacg tttctggatt ttttgcgcac gcattaaagc
     7201 cggtaaacta cgaggaatat cgtccagcgc cgaatgctgc gctttctgcg cgcgctcttc
     7261 ggttttgatt tgctcccaac gggcaagcac ttcactactg ttttcggcag aactgtcagc
     7321 aaaaacatgc ggatggcgac gctctaattt atcgctaatc gcagcgcaaa tatcattaaa
     7381 gtcaaagcgc ccttcttcct gagccatttg ggcgtaaaac accacctgga acagcagatc
     7441 gccgagttca ccgcgcagat cgtcaaaatc ttcacgggcg atggcgtcca gcacttcgta
     7501 ggtttcttca agggtgtaag gcgcaatggt ggcaaatgtc tgctctttat cccacgggca
     7561 gccgttttcc gggtcgcgca ggcgctgcat aatagtgagc aaacggtcga tttgattcat
     7621 tagaatgtcc tgaaaattgc gggtccgtca ggtggaaacc tgtgaccaga atagaagtga
     7681 gttagtaaca ctacccaatc agtacgttaa ttttggcttt aatgagttgt aattcctctg
     7741 gggcaaccgt tcctttcttc gttgctcctc ttgcccgcca ggcgatactt tttacctgat
     7801 cagctaacgc tacgccatca cgttcctgac cggataaaac aacttcgaac ggatatcctt
     7861 ttgattgcgt tgtacaagga acacacagac acatacctgt tttgttgttg tacatgaacg
     7921 gactcaggac aacagccgga cgatgtccgg cttgctcgct accttttgtc gggtcaaaat
     7981 caacccaaat cagatcgccc atatcgggta cgtatcggct taccattacc agacttcctt
     8041 atctttcggc tctccccagt cgatattctc gtggaggttt tccggcgtga tgtcgttgac
     8101 cagttcagca agcgtaaata cgggctcttt acgcactggc tcaataatta atttgccatc
     8161 caccaggtca atcttcactt catcatcaat attcagattg agcgcctgca ttaacgtagc
     8221 cgggatccgc accgccggtg aatttcccca acgctttacg ctactgtgga tcataaccct
     8281 ttcctcaaac cactatcata tgtagataca gtatatatca atctacattg tagatacgag
     8341 caaatttcgg cctaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg
     8401 agtttaccca gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg
     8461 gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg
     8521 ttggcgagaa tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg
     8581 accaccagcg aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct
     8641 ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg
     8701 cgtccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg
     8761 atgtggtgca tcaggttacc aacaccttca accactacac gaccgttatc tttactgcgg
     8821 ttttgcggcg tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg
     8881 gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc
     8941 ccgccaccaa tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga
     9001 tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc
     9061 cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa
     9121 cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc
     9181 ggctgtttct gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc
     9241 acaatgcgcc cgccaatttt tgccccgata cagcggtgtc cgacatcact gtggatgtgg
     9301 taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttaggcgta
     9361 aagacgtaca cccggtcgtc aaagacctgg ctgcgtactt cgtcgagcat ttcgccggaa
     9421 tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt
     9481 cccgaacgtg cgccgccagc agccgcgccc tctttatatt tccagtgcgc agcaacaccc
     9541 aactctgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc
     9601 ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa
     9661 tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa
     9721 caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag
     9781 gcgaggttct ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac
     9841 acttcagctt taacgccttc agctttcatc tcagcgcgca gatgaccgac aaactcttcg
     9901 atatagtgtt cgcggtcgag acggcgttca tgcaacagtt ttgcgatgcg tttgtattcg
     9961 gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca
    10021 agacggtttg ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacgcgttca
    10081 tcttccggcg catcttttac ttcacgcaga tgagcaatac gctccgccag tttgatgacc
    10141 acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg
    10201 gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg
    10261 ccgtgaataa ggttaacgac cgacttaccg acgctctcac gcagcacatc ttcgctgact
    10321 acgttggcat cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt
    10381 aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga
    10441 tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc
    10501 tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc agccttattg
    10561 atatgtgcac ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat
    10621 cggcaaatcg caactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt
    10681 gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt
    10741 cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga ataggttcca
    10801 gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt
    10861 tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat
    10921 aaaacgtcac attctgtaag cagttaagac gcgcattctg ctggcctttt tccaccagcg
    10981 ccggaacacc ttctacaccc accacactgg cagcttgtgt cgccaatggt agtgtaaagt
    11041 tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttccacctcc agccattcca
    11101 acgcacgcgc taccattttt tggttcacac ccgcattaac ctgaataaaa tcgcgcgggc
    11161 taaaagttaa gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga
    11221 gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca
    11281 gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc
    11341 tggttgcctg taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccaggc
    11401 atgccctgac tttgggcagc aatgcttcaa gttggggcac taaaatggga cattgtttaa
    11461 cgtcgacaat gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg
    11521 gtaagtagtt cagacttaaa cgcgcgcgac ggcgatagcc ccagggaaca tcggcgatca
    11581 cttcagagac ttcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta
    11641 aatccacgct ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg
    11701 gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt
    11761 ttttatcctc agtaacagta acttccgcgt tttcctgcgg caataatccg gggataaata
    11821 gcgttttgcc gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa
    11881 cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg
    11941 agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac
    12001 tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta
    12061 ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat
    12121 gccggtgcca gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa
    12181 aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt
    12241 cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt
    12301 gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact
    12361 cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac
    12421 gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag
    12481 ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc cagcgtgatg
    12541 atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc
    12601 ggtccgattc gcaacatggt gaatactgtc gaccgcatcc gtcgcgggca actcgacagc
    12661 cgagtggaag gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg
    12721 gcaatgtcgc tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc
    12781 gatctgcgtg aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa
    12841 aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag
    12901 ctgcgtacac cactgaatgg tgttattggt tttacccgcc tgacgctgaa aacagaatta
    12961 acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca
    13021 attattaatg atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt
    13081 attccattcc cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct
    13141 cacgataaag ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc
    13201 ggcgacccac tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc
    13261 accgagaatg gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg
    13321 cagattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc
    13381 ttattccagg ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg
    13441 ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat
    13501 agccagccga atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac
    13561 attattattg aagggccatc cacccagtgc ctcgccggta aacgcctggc ctatgtcgaa
    13621 ccaaactccg cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg
    13681 gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc
    13741 atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta
    13801 tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagtcaa tgctgaaaaa
    13861 ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg
    13921 cctgccctga cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa
    13981 agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc
    14041 ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggcg
    14101 gttgaacggg cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgcctgac
    14161 atggatggca ttcgggcctg cgagctcatc caccagctcc cccatcagca acaaacgccg
    14221 gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg
    14281 atgagcgatt atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc
    14341 tacaagcctg gcagcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg
    14401 gtgaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc
    14461 gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa
    14521 gttgaggaac agctggttgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg
    14581 catggcagtt gcggctatag cggtgtgccg cgtatgaaga atctctgcca acttatcgaa
    14641 caacagctac gtagcggtac taaagaagaa gatttggaac cggagctgct ggaactgttg
    14701 gacgagatgg ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt
    14761 cgagtagcaa tcggcttttt atgacgctgt tttcagattg gtgacaaagt gcctgtgttt
    14821 atgccggatg cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaataaatt
    14881 aaaaagcatg taggcctgat aagcgtagcg catcaggcaa ttttgtgttt ggcatcagtc
    14941 tcaaaccggc tccagataga gccggttttg gttttttgtc ttaacgcacc atgcacgggc
    15001 gcttgttatc gaacgtccag ccaggaatca gatactgcat tcccatcgca tcgtcacgcg
    15061 cgccaagccc gtgtttctga tacagctcat gggctttcat cacttgatcc atatcgattt
    15121 ctacacccag ccccggtttt tctggcacct gtaccagccc gcctttgatc tcaaacggtt
    15181 ctttggtcag gcgctgatta ccttcctgcc agatccagtg cgtatcaata gcagtaattt
    15241 tacccggtgc agcggcggca acatgggtaa acatcgccag ggaaatatcg aagtggttgt
    15301 tagagtgtga accccaggtc aggccaaatt catggcacat ttgcgccaca cgtaccgaac
    15361 cttgcattgt ccagaaatgc ggatccgcca gcgggatatc aacggattgt aacgagagcg
    15421 tatggcccat ctgccgccag tcggtggcga tcatattggt tgcagtcggc aggccagtcg
    15481 cgcgacggaa ctctgccatc acttcacgcc cggagaaacc ttgctccgca ccacacggat
    15541 cttctgcata agccagcgaa cctttcaggt atttgccgat tttaatcgct tcgttcagcg
    15601 accaggcacc gttaggatcg agcgtaatac gcgcctgcgg gaagcgttgc gccagtgcca
    15661 caatagactc ggcctcttct tccccggcca gtacaccgcc tttcagtttg aaatcgttga
    15721 agccatattt ttcatacgcc gcttccgcca ggcgcaccac cgcatccggc gtcatcgctt
    15781 cttcatgacg caggcgatac cagtcgcatg agtcatccgg ctggctttga tacggcagag
    15841 gtgtggcttt gcgattaccg acgaagaaca gataaccgag catttcgact tcgctacgct
    15901 gttgaccatc gcccagcagg gatgccacgt taacccccag atgctgcccc agcagatcca
    15961 gcattgccgc ttctatcccg gtaactacat gaatagtggt acgcaaatca aatgtctgca
    16021 aaccgcgccc gccggcatca cgatcggcaa aagtattacg caccagcgtc agaacgtttt
    16081 tgtattcacc cagcgtttta cccactacca gcggaattgc atcttccagg gttttacgga
    16141 ttttctcgcc gccgggaatt tcccctacgc cagtgtgacc agaattatct ttgataatca
    16201 caatattacg cgtgaagaac ggtgcgtgtg caccactcag attcatcagc atactgtcat
    16261 ggcccgccac cgggatgacc tgcatttcag taacaacagg cgtcgtaaat tgagaactca
    16321 tatttatgtc ctttttcaga atcaatgacg gccgaaaacg ggacgtttac ggtcaaaggt
    16381 ccagccgggg atcaggtact gcatcgggcc tgcgtcgtta cgcgcaccgc caggcagacg
    16441 tttataggcc tcatgtgcct tttgtacctg ttcccagtcc agttccacgc ccagaccggg
    16501 cgcatcagga acggcaattt ttccgttttt aatctccagc ggattttggg tcaggcgaca
    16561 atcgccctcc tgccaaatcc agtgggtatc gatagcggta ggattacccg gtgccgccgc
    16621 gcccacatgg gtaaacatcg ccagagagat atcgaaatgg ttgttggaat ggcagcccca
    16681 ggtcagcccc cagtcgtcgc aaagctgcgc cactcggact gcaccggaaa gcgtccagaa
    16741 gtgcggatcg gcaagtggaa tatctaccgc attgagcatc accgcatgac ccatttcgcg
    16801 ccagttggtg gcgatcatat tagtcgcgac gggcaagccg gtcgcccgcc gaaactccgc
    16861 catcacttca cgtccggaga agccctgttc tgcgccgcat ggatcttcgg cataggtaag
    16921 aacatcattc agccctttgc acagagaaat ggcttcatca agcagccatg caccgttggg
    16981 atcaacggta atccgcgcat ccgggaagcg tttcttcaat gcacgaacag tgtcgatttc
    17041 ttgctcgcca ggtaacacgc cgcccttaag tttgaaatct ttaaagccgt agcgatcctg
    17101 tgaggcttcc gccagacgca caacggcttc gctgttcatc gctttctgat ggcgcaactg
    17161 ataccactca tggttgcccg gcgtattttc cagataagga agatcggttt tggtccgatc
    17221 accgatataa aacagataac cgaggacggt aatagcatcg cgttgcttgc ctggccctaa
    17281 cagttcgcag accggaacat tcagcgcctt acctagcagg tcaagcaagg cggcttccag
    17341 cgccgccacg gcgttaacgc gcaattcaaa ggtccaggcc cctttaccga aggtatcaaa
    17401 atcggctgct tgattacctt tatgcacctg ctgaaccact ttattcaggc gcgcaacttc
    17461 ctagcccagc accatcggaa tagcatcgac caatgtctga taaatcacct ctccgcctgg
    17521 cgcttcacca atgccggtat gcccggcgtt atcggtgagt accacaatat tgcgagtgaa
    17581 atatgcgtta tgcgccccac caatattaag caacatgctg tcgtacccgg cgaccggaat
    17641 gactttcata tcagtaataa caggactgga ttgtgtcgtc atcttattgc cccacgacag
    17701 gtttcagctc gatacgcttg atatcgccca ccagcaccag gtagctcagt accgcgatta
    17761 aggcatgaac accaacataa atcagcgccc cattaaacga gccagtcgtg ccaacgatat
    17821 aaccaattgc gattggcgtg acgatgccag aaatgttacc gaacatgttg aacaggccac
    17881 cggaaagacc gctgatctct tttggcgcgg tatctgccat tactgcccaa cccagcgccc
    17941 caatgccctt accgaagaag gccagcgcca taaagccgat gatcatccac tcaacgttga
    18001 cgtagttgca gaacaccatc accatcgaca gcaacatgcc cattacgatc ggtgttttac
    18061 gcgcaatgtt cagcgatccc gtgcggcgca tcagccagtc ggaaataatc ccacccagca
    18121 caccaccgat aaaaccacaa accgccggaa cggaagccac aaagcccgct ttcagaatcg
    18181 acatcccacg cgcctgcacc agataaaccg gaaaccaggt aatgaaaaag taagtcaggg
    18241 cgttgataca gtactgaccg atataaacgc cgatcatcat ccgtgacccg agcagttgtt
    18301 tgatctgccc ccacttcacg ctgaacggca ctttaacttt ggtgttttgc tgatccatat
    18361 tgatcagcgc gccacccgcg gcgatgtact ccagctcttt ctgatttacc cccggatgtt
    18421 gatttggctc gtggatgact ttcaaccaga taaagctgat ggcaatgccc agaccgccca
    18481 taaagaagaa gacgtgtgac cagcccactt catgcgtcag ccagcccata atcggcgcga
    18541 agatcaccgt tgcgaagtat tgagcggagt taaaaatcga caccgccgtt cccctttcct
    18601 gcgccggaaa ccaggccgca acaatgcgac tgttgccggg gaaagatggc gcttcagcaa
    18661 gcccgaccag gaagcgcaga gtaaacaggg caacgataat gccgaatcca ctaaagatat
    18721 cgacgaagcc ttgcagcaag gtaaacatcg accagataaa gatcgaccag aagtagacgc
    18781 gttttgaacc aaaacgatcc agcaaccaac caccagggat ctgcccgata acataagccc
    18841 atgagaaggc agagaacaca tagcccattc ccacgggatc aaggccgata tctttggcca
    18901 tttccgaacc ggcaatagaa agcgtagcgc ggtcgccgta gttgaaggat gtgacgataa
    18961 acaacatcac cactatccag taacgagcat tcgtgcgttt ttccacactg ctcgcagcct
    19021 gacttaaaga actcattgtt gcactcctga aaattcgcgt tagccacgct cactctggac
    19081 tgcgacatcg ccaggaaatc agaggtgacg tagggtgttt tttgccgttt ttataggtcg
    19141 ttcgccgaat acggcgcgtg tttatatctg gcaatagcag tataaaaagc gcgccatagc
    19201 ggctcaccgt gcaacaacac aacattaatg cgttcaatga ggcccgattt tggcattagc
    19261 cctggacggt ggaatccact tcacggaaat gaaaacaaga acaagaaagg aagggttaaa
    19321 acgaagaaat aaagaagagt atgaaatgga tcgcttgact ccaggcaaac gccagtaaaa
    19381 atccgcgcta tgaagcagtt tttactggca tttgcctgaa aagattcgat tcagcaccgc
    19441 taaaacgaca tttaccgctc gctgaacata tcaggacaac agcgtgcccc actgttcgac
    19501 ccacggattt gattccgttt ccggttccgg gttctcactg gcgtcaatca acagcatttc
    19561 gccaacccgc tgagcgctct gttcctgcag caaggcatcg aactgtttgc cgccattgca
    19621 gaaattcaca taactactgt cgccgagcgc aatcacgcca taacgcagat tcggctggaa
    19681 gcccagacta tctttgattc cctgaaagag cggcacaatg ctatcaggaa ggtcgccctg
    19741 cccggtcgtg gacgtaacca ccagaacata cttatcctga tagggcagcc agtcgcttaa
    19801 ttcaggatct tcaaataccg ttgctttgtg gccctgtgcg gtcagaattg cttctgcttc
    19861 ttccgccact aacagcgaat ttccgtacat ggtgccgaca aaaataccaa tttccgccat
    19921 accgttttct ccctggatta ggaacttctc tctccatcct gacccgatgg cgcactgaac
    19981 tcaacccttt cattttcagg aagcagaccg cgccagccaa attgtgataa cgcctgcatc
    20041 caggtgtcgt ccaggcccgc gtgaatagtc agcggctcac cagtaaaagg atgcgtcaat
    20101 gacaactgac tggcatgcag cattaaccgc tgaagaccaa aatgctcagc accgctgcga
    20161 ttctggcgta aatcgccatg tttgctatcg ccaataatcg gatgacgcaa atgggcaaga
    20221 tgtcggcgaa gctgatgttt gcgtccggtt ttcggctcca gttccaccag gccgtagcgc
    20281 gtggtcgggt aacgtccggt cgctaccggc atttctacgg tcgccagacc gcgataatgc
    20341 gtcactgctg gctgcgggcc tttatcttcg cgggcaaatt tatcagcgat tttgtccagt
    20401 tcttccacca gtggataatc cagcaccgct tcttccatca accagccgcg cacaatcgca
    20461 tggtaacgtt tctggatttg gtgctgttca aactgttgtg ccagcagccg tccggcctcg
    20521 ctggataatc ccatcaacaa cacaccagaa gtgggtcggt ccagacgatg agcagtaaaa
    20581 acatgctggc ctatctggtc acgcacggtt tgcatgacca ctactttctc gtcgcgatcc
    20641 agccagctac ggtgaaccag ccagccggag ggtttattta ccgcaaccag ccattcatcc
    20701 tgatagagta tttccagcat tagctcgcat catccgcaaa aagagcatcc agtttttcca
    20761 actcagccag aataagcgcg cgttgcggat ggtccgtcgc cagcgccatt tcataatagg
    20821 gtgcaacggc aaaagcgccc ggtaacggct ggttgttatt taacaaatcg tgcattcgcg
    20881 ggatcagcac ccactgcaac cactccagtg gttccatggt gtccataaag aacggttggg
    20941 tactattaaa ttgatgcggc tggggttcat cgtttcgcca gtgctgatgt tcacgcagta
    21001 acgcttcaag cgcctgcaac tggagacgaa cgcggtcatg agtggtcata aagcaacctc
    21061 aataagaaaa actgccgcga aggatagcag gaataaaaaa agggagcact gtattcacag
    21121 tgctcccggt tcgtttcgca gcattccagc tacttttgtt gctccctgct catccttgac
    21181 aacttttcct ctggccatgc ggccaatcgt tcatcctgaa ctattgcttc ctgctcacac
    21241 caccccgatg tgatacttca tcctgaagtg tcccaggcct tcctgaccca ccgaatcatc
    21301 ctgaccggtt ctcattctcc atcctggagg tgtcctttaa cgcgtcctgc gtcatcctct
    21361 tcgcttcatc cagaagcctt tccctgaaac accatcctgg tgtgtccttc agaagtgtca
    21421 tcatcctgat gttcacttcg ttgtctgact ccctgtcgac gaagatagaa tcgtcttttt
    21481 cgcgaactct tacaaggcgc ttacagacaa tgccttaagg aaaatttcat atgaaagtta
    21541 ggaattaaca agcaacatgt tgtttttaaa tgaaaataac gattcttatc tttttaagta
    21601 ttcgcttatt tcctggcgat ctcctacaga gtctgtaaga gatctctcac aaccagatgg
    21661 ggtatttgga ttacagaaga ggcttaagtt gattaaggaa ttccgcaaga tttggggcta
    21721 aatgcgtccg tttacgtgtt cccagcgtct ctttacacac ttcaccggag agattacaca
    21781 ccgagatgac ctctaactct tcttccagcg tcgcgataaa tagcgtgggt ggcagtttta
    21841 gccttttctg agtcaccaga tgaccaatca gattttcctg cacccgacgg aagtcgtctt
    21901 cactccaggt ttgcagcaat gtcagtttga tatcgccaaa ctgcgcgtgc atatccccgg
    21961 caaactgagt cgtataaaag gtatgaattg tgggttgtat cacaatatca aaagcgcgtt
    22021 caaccgcgtt tacattttgt tccccggtaa acggctgggg ttgccagtat acggcatctt
    22081 cagtagttga aataatgcac ggtgaaggaa cgccgtacag ttcctcactt aacggccagc
    22141 ttttatgctc ttcatgccac gcatcgcagt agcgtgcagt aaaatctttc agggcttgtg
    22201 cggtcaaatc gtccactaat ttctctcttc acttaaacca gatacacttg tcgtttagtt
    22261 tatctggttt atgacggtga aacatgtctt cttatgcaaa ccatcaggca cttgcgggcc
    22321 tgactcttgg aaaatcaacc gattaccggg atacctatga cgccagccta ctgcaaggcg
    22381 ttccacgcag cctgaatcgc gacccgctgg gtctgaaagc ggataacctg ccttttcaag
    22441 gtacggatat ctggacgctg tatgaacttt cctggctgaa tgcgaaaggt ttgccgcagg
    22501 tcgctgtcgg tcatgttgaa cttgattaca ccagcgtaaa tctgattgag tcgaagagtt
    22561 ttaagctcta tctcaacagt tttaaccaga cgcgttttaa taactgggat gaggtgcgcc
    22621 agacgctgga gcgcgactta agcacttgcg ctcagggtga ggttagcgtg gcgttatatc
    22681 gtcttgatga actggaaggc cagccgatag gtcatttcaa tggcacttgc attgatgacc
    22741 aggatatcac tatcgataac tatgaattca ctactgacta tctggagaat gccaccagtg
    22801 gtgaaaaagt agtggaagag acgctcgtca gccacctgct gaaatcaaac tgcctgatca
    22861 cccatcaacc agattggggt tcgatccaaa ttcagtatcg tggacgccaa attgacagag
    22921 aaaaactgct gcgttacctg gtctcattcc gtcatcacaa cgagttccac gaacagtgcg
    22981 tggaacgcat ctttaatgac ctgttacgct tctgccagcc agagaaattg agcgtttacg
    23041 cacgttatac ccgtcgtggc ggtctggaca ttaacccgtg gcgcagtaat agcgattttg
    23101 tcccatcgac cacaagactg gttcggcaat aaattttttc tcaattttgc gtgctggatt
    23161 cacgcagaag gttgtgaaag gtcatcaggc agggctattg taatcaaagg gaatgacgat
    23221 attcgtccca taaggagttt ttcttgatta cacatattag cccgcttggc tccatggata
    23281 tgttgtcgca gctggaagtg gatatgctta aacgcaccgc cagcagcgac ctctatcaac
    23341 tgtttcgcaa ctgttcactt gccgtactga actccggtag tttgaccgat aacagcaaag
    23401 aattgctgtc tcgttttgaa aatttcgata ttaacgtctt gcgccgtgaa cgcggcgtaa
    23461 agctggaact gattaatccc ccggaagagg cttttgtcga tgggcgaatt attcgcgctt
    23521 tgcaggccaa cttgttcgcg gttctgcgag acattctctt cgtttacggg caaatccata
    23581 ataccgttcg ttttcccaac ctgaatctcg acaactccgt ccacatcact aacctggtct
    23641 tttccatctt gcgtaacgct cgcgcgctgc atgtgggtga agcgccaaat atggtggtct
    23701 gctggggcgg tcactcaatt aacgaaaatg agtatttgta tgcccgtcgc gtcggaaacc
    23761 agctgggcct gcgtgagctg aatatctgca ccggctgtgg tccgggagcg atggaagcgc
    23821 cgatgaaagg tgctgcggtc ggacacgcgc agcagcgtta caaagacagt cgttttattg
    23881 gtatgacaga gccgtcgatt atcgccgctg aaccgcctaa cccgctggtc aacgaattga
    23941 tcatcatgcc ggatatcgaa aaacgtctgg aagcgtttgt ccgtatcgct cacggcatca
    24001 ttatcttccc tggcggtgtg ggtacggcag aagagttgct gtatttgctg ggaattttaa
    24061 tgaacccggc caacaaagat caggttttac cattgatcct caccggcccg aaagagagcg
    24121 ccgactactt ccgcgtactg gacgagtttg tcgtacatac gctgggcgaa aacgcgcgcc
    24181 gccattaccg cataatcatt gatgacgccg ctgaagtcgc ccgtcagatg aaaaaatcga
    24241 tgccgctggt gaaagaaaat cgccgtgata caggcgatgc ctacagcttt aactggtcaa
    24301 tgcgcattgc gccagatttg caaatgccat ttgagccgtc tcacgagaat atggctaatc
    24361 tgaagcttta cccggatcaa cctgttgaag tgctggctgc cgatctgcgc cgtgcgttct
    24421 ccggtattgt ggcgggtaac gtaaaagaag tcggtattcg cgccattgaa gagtttggtc
    24481 cttacaaaat caacggcgat aaagagatta tgcgtcgtat ggacgacctg ctacagggtt
    24541 ttgttgccca gcatcgtatg aagttgccag gctcagccta catcccttgc tacgaaatct
    24601 gcacgtaatc tcccctcccc cggtgacgtt tgttgccggg ttttctcgtt tttggtcact
    24661 tactcatcaa ctcatttcat ttgttatatg aatgtttctt accaccctca cgcgacaaat
    24721 atcatcacag ttaatatgtc atacaattta tattgcaacg caaacgtttc cct
//
LOCUS       DAZKIF010000069        24308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZKIF010000069 DAZKIF010000000
VERSION     DAZKIF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.069"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(98..979)
                     /gene="prmA"
                     /locus_tag="ACHOTW_002724"
     CDS             complement(98..979)
                     /gene="prmA"
                     /locus_tag="ACHOTW_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9844801.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(991..2442)
                     /gene="panF"
                     /locus_tag="ACHOTW_002725"
     CDS             complement(991..2442)
                     /gene="panF"
                     /locus_tag="ACHOTW_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP9844802.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2432..2674)
                     /locus_tag="ACHOTW_002726"
     CDS             complement(2432..2674)
                     /locus_tag="ACHOTW_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP9844803.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2783..4132)
                     /gene="accC"
                     /locus_tag="ACHOTW_002727"
     CDS             complement(2783..4132)
                     /gene="accC"
                     /locus_tag="ACHOTW_002727"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9844804.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4143..4613)
                     /gene="accB"
                     /locus_tag="ACHOTW_002728"
     CDS             complement(4143..4613)
                     /gene="accB"
                     /locus_tag="ACHOTW_002728"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9844805.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5591..6565)
                     /locus_tag="ACHOTW_002729"
     CDS             complement(5591..6565)
                     /locus_tag="ACHOTW_002729"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP9844806.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6717..8657
                     /gene="csrD"
                     /locus_tag="ACHOTW_002730"
     CDS             6717..8657
                     /gene="csrD"
                     /locus_tag="ACHOTW_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP9844807.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8962..10005
                     /gene="mreB"
                     /locus_tag="ACHOTW_002731"
     CDS             8962..10005
                     /gene="mreB"
                     /locus_tag="ACHOTW_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP9844808.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10071..11174
                     /gene="mreC"
                     /locus_tag="ACHOTW_002732"
     CDS             10071..11174
                     /gene="mreC"
                     /locus_tag="ACHOTW_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9844809.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            11174..11662
                     /gene="mreD"
                     /locus_tag="ACHOTW_002733"
     CDS             11174..11662
                     /gene="mreD"
                     /locus_tag="ACHOTW_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9844810.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11671..12264
                     /gene="yhdE"
                     /locus_tag="ACHOTW_002734"
     CDS             11671..12264
                     /gene="yhdE"
                     /locus_tag="ACHOTW_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP9844811.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12254..13723
                     /gene="rng"
                     /locus_tag="ACHOTW_002735"
     CDS             12254..13723
                     /gene="rng"
                     /locus_tag="ACHOTW_002735"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP9844812.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13791..17591
                     /gene="yhdP"
                     /locus_tag="ACHOTW_002736"
     CDS             13791..17591
                     /gene="yhdP"
                     /locus_tag="ACHOTW_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP9844813.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17747..19192
                     /gene="tldD"
                     /locus_tag="ACHOTW_002737"
     CDS             17747..19192
                     /gene="tldD"
                     /locus_tag="ACHOTW_002737"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP9844814.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19326..20255)
                     /gene="aaeR"
                     /locus_tag="ACHOTW_002738"
     CDS             complement(19326..20255)
                     /gene="aaeR"
                     /locus_tag="ACHOTW_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP9844815.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20438..20641
                     /gene="aaeX"
                     /locus_tag="ACHOTW_002739"
     CDS             20438..20641
                     /gene="aaeX"
                     /locus_tag="ACHOTW_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP9844816.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20649..21578
                     /gene="aaeA"
                     /locus_tag="ACHOTW_002740"
     CDS             20649..21578
                     /gene="aaeA"
                     /locus_tag="ACHOTW_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP9844817.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21584..23551
                     /gene="aaeB"
                     /locus_tag="ACHOTW_002741"
     CDS             21584..23551
                     /gene="aaeB"
                     /locus_tag="ACHOTW_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP9844818.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23643..23915
                     /locus_tag="ACHOTW_002742"
     CDS             23643..23915
                     /locus_tag="ACHOTW_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP9844819.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23971..24234)
                     /gene="yhcN"
                     /locus_tag="ACHOTW_002743"
     CDS             complement(23971..24234)
                     /gene="yhcN"
                     /locus_tag="ACHOTW_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP9844820.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5751 a         6139 c         6611 g         5807 t
ORIGIN      
        1 tttggcacgc aaatttgcgt aaataatcgg caaatttacg tgccttttgt gagcttgctc
       61 gcacttcgcc ccgcgtcacc ctatggcgat gcgaaggtta attcttacga ccggtaatac
      121 ggcaccactc ttctttttcc acgaccgggt ccagagcgaa gctgtcggca taggcttcgc
      181 aaacgctctc cgcctggctt gccagaatac cagaaaggcc cagtaagccg cctgaaactg
      241 gcaggacgct aattaacggt gccagttcac gtaatgggcc tgcaaggatg ttagcgacca
      301 ccacgtcggc tttcatttct tctggctgat ctttcggtaa gtagagttcc agacggtcag
      361 aaacgccatt acgttcggcg ttatcgcggc tggcctgaat cgcctgcgga tcgatatcaa
      421 taccaatggc ttttgctgca cccagtttca gcgccgcgat cgccagaatg ccggaaccac
      481 agccaaagtc gatgactgtt ttaccggtta aatcgaggct gtcgagccat tgcaggcaca
      541 gagaggtggt tggatgggta cccgtaccaa acgccagccc tggatctaac atcacgttga
      601 cggcgttttc gtccggcaca tcacgccagc tagggcagat ccacagtcgt tcgccaaagc
      661 gcatcgggtg gaaattatcc atccattcgc gctcccagtc tttatcttct agttgttcga
      721 ttttatgcgc gaagcctgcg ccgagcagcg gatggttttc cagaatcgcc accacgtcgt
      781 tcatatcggt ttcagcgtcg aacagaccaa tcacatcggt gtcgccccac aggcgcgttt
      841 cgcccggcag cggctcaaat actggcgtat cgtgggtatc ctgaaaagtg atagaaacgg
      901 cacccgcttc catcagcgca tcgctaagat cttccgcgtt cgcgccggtg gtgttcagtt
      961 tcagttggat ccaaggcatg gcaaaactct ttatttatca gtagtcaaaa cggtagcttg
     1021 cgggacggat gtaccgaaac ggtttccgac caggaaagcc agcaaactta gtagtaacga
     1081 gggcacgata gggtggaagc ccaggtactg aatattcagc gtcgcgagta cggcatacag
     1141 cacgccgcca acgatcatcg cacttagcgc gcctttggcg ttggcgcgtt cccagtaaag
     1201 acccagcacc agcggccaca ggaaaacggc ttccagccca ccgaaggcca gcaaattcag
     1261 ccagatgatc atttctggcg gcttccaggc ggcaagcagc agcaacgcgc cgagaactaa
     1321 cgtaattacc gccgacatcc gcttcagacg cgtctcgttt tgcatttgat ccggacggat
     1381 attcagatag agatctttaa tgatcgtagc ggaactttgt agcaattggg cgttaattgt
     1441 cgacatgatc gcagccatag gtgcagccag aaagatcccg gcagcaaacg gtggcagcac
     1501 ttttaccatt aacgtcggga tcaccagatc cggtacggtg agatcgggga tcactgcacg
     1561 acctaacgct ccggctaggt gcataccgaa catcaggatt gcgactacaa tcgtaccgat
     1621 gatgatcccc cggtgtactg ctttgctgtc tttataagag atacagcgca ccgcagtatg
     1681 cggcaggcct ataacgccaa agcacaccag tacccagaac gacgtcataa aggcaggcga
     1741 cagaatatcg tcagcgcctt gtggcgtaac cagttgcgga tcgatggttt gcaaggtctg
     1801 tactgcgttg cttaagccac cagcggcatg taccacgcca ataagcagca caacggtgcc
     1861 aatcagcatc acaagccctt gcatggtgtc gttcagcacg ctggcgcgaa agccaccaaa
     1921 ggcggtatat aacgcaatgc tgataccaaa aatcagcagc ccggtttcat aaggaatacc
     1981 cgccgcggtt tccagcaggc gcgcaccgcc gataaactgc acggtcatcg caccaacgaa
     2041 cgcaaccagc aaactcaaac tcgccagcca caccagaaga cgactctggt agcgggcaaa
     2101 cagcatatcg ttcagcgtca ctgcattgta gcggcgcgca agaatcgcaa acttcttgcc
     2161 gagaataccg agtgaaagcc agactgccgg aagctgaatc atcgccagca atacccagcc
     2221 aaggccgtat ttgtaagcag ctccaggccc gccgataaac gaactggcac tgatataagt
     2281 cgcggtgagc gtcatcgcca gcacaatacc gcccatagag cggctgccga ggaaatactc
     2341 attaaggaag gtgccggtgc tccgtttacg catcgcataa accgagatac cgaacaccac
     2401 caccagatag gcgaccagcg gtagaattac ttcaagctgc atcgtcatcc tccagcggga
     2461 tatcgcgata gataaatttc accatcgccc agcacagtcc aataaacagc agcggcgtca
     2521 ggatgcaggc catctcaaac cagcgcggaa agccggtaaa accgggggca acgccaggta
     2581 agtaagcggc tactaaccaa actgccagat acaaaagggt cagccccagc gcccagcgcg
     2641 cctctttatg ggcctgaaca aaacgagtgt ccattttttg tccctgatgg gtgaagaaag
     2701 cgaggattgt accttatggg tgttgtcgat ccccagtaat aaaaaaggcc ggaaaatccg
     2761 gccttttgac gcttttgcag tcttattttt cctgaagacc gagttttttc tccagatagt
     2821 ggatgttagt gccaccatgc tggaagttct cgtcattcat gatgcggatc tgcagatcaa
     2881 cgttggtttt gataccgtcg atgatcagct cctgcagcgc attcttcatg cgggcaatcg
     2941 ccacgtcacg gttttcaccg tagcaaatca gcttaccgat cattgagtca tagtacggcg
     3001 gtacggtgta gcccgcgtag atatgagact cccaacgtac gccaaaaccg ccaggtgcgt
     3061 ggaaacgggt gattttgccc ggacttggca ggaaggtgtt cggatcttcg gcgttgatac
     3121 gacattccac cgcatggccg cgaacgtgaa cttcttcttg cttgatcgac agcggttgac
     3181 cggcagcgat acgcagctgt tctttgatca ggtcaacgcc ggtgatcatt tctgtaaccg
     3241 ggtgttctac ctgaatacgg gtgttcattt cgatgaaata gaactcgccg ttttcgaaca
     3301 ggaactcgaa agtacctgca ccgcgatagc cgatatcaac acacgcttta gcgcaacgtt
     3361 cgccgatgta gcgacgcagt tccggggtaa tgcccggtgc tggtgcttct tcgaccactt
     3421 tctggtggcg gcgctgcatg gagcagtcac gttccgccag atagatagag ttgccctgac
     3481 cgtcagccag tacctgaatc tcgacgtggc gaggattttc caggtatttc tccatgtaaa
     3541 ccatatcgtt gctgaaagca gctttcgctt ccgcacgggt catggagatg gattgtgcca
     3601 gttcagcgtc gccgcgcact acgcgcatac cgcgaccacc gccgccgccg gaggctttga
     3661 taatcaccgg ataaccaatg cgtttagcaa tggcacggtt tttatccata tcgtcgccca
     3721 gcgggccgtc agaacccggt acgcaaggga cgcccgcttt tttcatcgcc gcgattgcgg
     3781 atactttgtc gcccatcagg cgaatggttt ctgctttcgg gccaatgaag ataaagccgg
     3841 agcgttcaac ctgctcggca aagttggcgt tctcggagag gaagccgtaa cccggatgga
     3901 ttgctactgc gccggtgatt tcagcggcgc tgatgattgc cgggatgttc agataacttt
     3961 ttactgacgg agcagggcca atacagaccg tttcatctgc cagtaatacg tgttttagat
     4021 cgcgatccgc gctggagtgc acagcgacag tcttgatgcc cagttcttta caggcacgaa
     4081 gaatacgcaa tgcaatctcg ccgcggttgg caataacaat tttatccagc atgttcgcct
     4141 cgttactcga tgacgaccag cggctcgtca aattctaccg gttgtccact ttcgaccaga
     4201 attgctttca cggtaccgga tttgtccgct tcgatctggt tcatcatttt catggcttca
     4261 acgatgcaca gggtatcgcc cacgttgact ttctgaccca cttcgatgaa cgcttttgca
     4321 tccgggcttg gggtgcggta gaaagtacca accatcgggg aacgtacgat gtgaccactg
     4381 atttccgctg ctgctggcgc ttccatggaa ggaacggtcg ccggagcggc tgcgttagat
     4441 tgagctggct gctgcatcat tggtgcagcg taagcttgtt gcatcacagg gaaacttgcg
     4501 gcaggagctg cacggctaat gcgtactgac tcttcgcctt cagaaatttc cagttcggag
     4561 atgcctgatt cttcaaccag ctcgatcagt tttttaatct tacgaatatc catgagtggg
     4621 ttccgtactc tttgtttagt gtgattgtga caggcgtttc accgccgtct gtaaagcgta
     4681 tgccccgaca gctgtatgca tagcgataaa ttccagcagg tcgggacgcc ggtctatttt
     4741 gccccagagt gtcaatgtta gacttgacgg acattgtgca gcgatctgct gctatcctcc
     4801 ggcaaaaaac aaaatatacc ttcgttgcgc caatgtcacc ttttccgcag cgcaaaaact
     4861 gcgtcaggga ggacgaggtc gcacattata acgatttcgt agcaattggc agctaaatac
     4921 tggtcttatc agggaagata atcaacaact aacatgtaaa taaccttcaa caccgtgtaa
     4981 tttgcaacaa gccgcacaat tcacgaaatt agcgccatca tcgacggaac ttcttataac
     5041 gtaaggtgaa aagctgtaaa gccagccctg cgaagatgag gggctgcaat gagataatcg
     5101 tcacagacca caaataatgt agggatgcca ggatcgcgac aagatagacg acattacgca
     5161 gcggttgctg gggattaccc aattttcact gcatcgcctg gatagatgtg aatgctaaag
     5221 cacgcaaaat taaccgattg acaatgctca gcattaaata ggttcgagtg attaatttgt
     5281 agtcaaacaa aatcagatta ttcacctcat ggtaccagaa ataagtaagg ggttaagcgt
     5341 agcgtcgccc aggcaaagca ccataaacct aacaggcgac ggaggcgtat caataatagc
     5401 tgtttagtgg agtatcaatg cgcatcaatt tatggtcagt cgaaatcatg actttccagt
     5461 ttggatgatg ttgtacaact tcatgaccaa ggtgttcgac gaaagctgtc cggctaaccg
     5521 cttttcccgt taaccgattg aataatgatt tcaaaatgag ttccctctct tattattcct
     5581 tgctaaatgg ttagttaacc ttcaccagcg tgcgaccctg gatctggtta ttaatgatgg
     5641 cctcggcaaa gttcggtgcc tctgacagag atatctcttt tgccgcctgg gtatagaatg
     5701 attccggtaa atcggcgacc agtcgctgcc aggcttgtgc gcggcgttct ggtggcgtca
     5761 ttactgaatc caccccttgc aaacggacat tacgcagaat aaatggcatg accgtggttg
     5821 gcagagtaaa accacccgcc agaccacagg ccgccacgca gccgccgtaa ttcatttgcg
     5881 ccagcacttt tgccagcact ttgtcgccaa cagtgtcaat tgccccagcc cagacttgtt
     5941 tttccagagg acgggattcg gcaaactcat cacgagggag aatacggctg gcacctaaac
     6001 ttttcagata ttcatgggta ctttcgcgac cggaaacggc aacgacctga taacccaact
     6061 tatgcagcag cgccacggcg gtactgccga cgccaccact ggcacccgtc acgacaatct
     6121 ccccgtcctg cgggcgaaca ccggcatctt ccagcgccat cacacacagc atggcggtaa
     6181 aaccggcagt accgataatc attgctttac gcgcgtccag cccttgcggc atggcaacca
     6241 gccagtcacc tttcactcgc gcctgctccg ccagcccacc ccagtggttt tcaccaacgc
     6301 cccagccagt gagtaacacc tcctgaccgg catgaaaacg cggatcttcg ctggtgcgta
     6361 cagttccggc aaaatcgatc ccaggaatca tcggaaaatt acggatgatt tttcccttac
     6421 cggtaatcgc cagcgcatct ttatagttca ggctcgacca gtgaacatcg accgtgacat
     6481 cgccctccgg caggcgactt tcgtccagag tctgtactga tgcgagagtt ttgccgtcct
     6541 gctgttctaa aagtaacgcc tgcataagtg gtcctcatgt gcatgatgga ttggaaaata
     6601 atttctgaag actatactcg ctaatggaaa agcaaagccg atgaagcgca ataaattgcg
     6661 agataaatct gatttgctag tatgcccgct tcctcactat cggagttaac acaaggatga
     6721 gattaacgac gaaattttcg gcctttgtta cgctgctcac cgggttaaca atttttgtga
     6781 ctttgctggg ctgttcgcta agtttctaca acgccattca gtataagttt agtcatcgcg
     6841 ttcaggcggt ggcgacggcg attgataccc accttgtgtc gaatgacttc agcgcattaa
     6901 ggccacaaat taccgaatta atgatgtcgg cagatatcgt tcgtgtagac ctgctccatg
     6961 gtgataagca ggtttatacc ctggccagaa atggtagtta tcgtccggtt ggcaccagcg
     7021 atctgtttcg tgaactgagc gttccgttga taaagcatcc ggggatgtcg ttgcgtctgg
     7081 tttatcagga tccgatgggc aactatttcc attcgttgat gaccaccgcg ccgctcacag
     7141 gggcgattgg ctttatcatt cttatgctct ttctggcggt acgctggtta caacggcaac
     7201 ttgccgggca agaattgctg gaaacccggg ctactcgtat cttaaacggt gagcgtggct
     7261 ctaatgtgtt gggaaccatc tatgaatggc cgcccagaac cagcagtgcg ctggatacgc
     7321 tgcttcgtga aattcagaac gcacgcgaac aacacagccg tcttgatacg ctgatccgct
     7381 cttatgccgc acaggacgtg aaaaccggcc tcaataaccg actctttttc gataatcagt
     7441 tagcaacgtt actggaagat caggagaaag taggtaccca cgggatcgtg atgatgattc
     7501 gtctgccgga tttcaatatg ttgagcgata cctgggggca cagccaggtt gaagaacagt
     7561 tcttcactct gacgaatctg ctgtcgacat ttatgatgcg ctaccctggc gcactgctgg
     7621 cgcgttacca ccgcagtgat tttgctgcgc tgttaccgca ccggacgtta aaagaggcag
     7681 agagcatcgc cggtcagtta atcaaagccg tcgatacctt gccgaacaat aaaatgctcg
     7741 atcgcgacga tatgatccac attggtatct gcgcctggcg tagtggtcag gataccgagc
     7801 aggtaatgga acatgcagag tctgccacgc gtaatgcggg attgcagggc ggcaatagct
     7861 gggctattta cgatgactcg ttgcctgaaa aaggacgcgg taatgttcgc tggcgtacgc
     7921 ttatcgagca aatgctcagt cgcggcggcc cgcgccttta tcaaaaaccg gcggttactc
     7981 gcgaaggtca ggttcatcat cgcgaactca tgtgccgcat ctttgatggt aatgaagagg
     8041 ttagctcggc ggagtatatg ccgatggtct tgcagtttgg cttatcggaa gagtatgacc
     8101 gtctgcaaat cagccgtctt attccactat tgcgttactg gccagaggaa aatctggcga
     8161 ttcaggttac cgttgagtcg ctgattcgcc cgcgttttca gcgttggctg cgcgatacgt
     8221 taatgcaatg tgaaaaatca caacgaaaac gcataattat tgaacttgca gaggccgatg
     8281 taggtcaaca tatcagtcgt ttacaacctg ttattcgttt agtgaatgct ttaggggtac
     8341 gggtagccgt caaccaggct ggtttgacgc tggtaagcac cagttggatc aaagaactta
     8401 atgttgagtt actcaagctc catccggggc tggtcagaaa cattgagaag cgaacggaga
     8461 accagctgct ggttcaaagc ctggtggaag cctgctcagg gaccagcacc caggtttacg
     8521 ccaccggtgt gcgttcgcga agcgagtggc agaccctgat tcagcgcggt gttacaggcg
     8581 ggcaagggga ttttttcgcg tcctcacagc cacttgatac taacgtgaaa aaatattcac
     8641 aaagatactc ggtttaacct gccgtttaat ccgttttcac gtagaataac gcgcgctgcg
     8701 tctcatggga gtgtgcttgt ctgctcgcca gattgttgca gcacatatgc agatgaatga
     8761 ccttacgcgg ttgcaaacag gcgaggaatg ctgctgatgc attaagcctt tctggactca
     8821 ggcagagatt tgtaacaaag gaaacgaact gcactaattt tcaccgtagc agatgatttt
     8881 tgcgccttgt cgctgctgcg tgtggttggt aaagtaagcg gattttcttt tccgccccag
     8941 ctttcaggat tatcccttag tatgttgaaa aaatttcgtg gcatgttttc caatgacttg
     9001 tccattgacc tgggtactgc gaataccctc atttatgtaa aaggacaagg catcgtattg
     9061 aatgagcctt ccgtggtggc cattcgtcag gatcgtgccg gttcaccgaa aagcgtagct
     9121 gcagtaggtc atgacgcgaa gcagatgctg ggccgtacgc cgggcaatat tgctgccatt
     9181 cgcccaatga aagacggcgt tatcgccgac ttcttcgtga ctgaaaaaat gctccagcac
     9241 ttcatcaaac aagtgcacag caacagcttt atgcgtccaa gcccgcgcgt tctggtttgt
     9301 gtaccggttg gcgcgaccca ggttgaacgc cgcgcaattc gtgaatccgc gcagggcgct
     9361 ggtgcccgtg aagtcttcct gattgaagaa ccgatggctg ccgcaattgg tgctggcctg
     9421 ccggtttctg aagcgaccgg ttctatggtg gttgatatcg gtggtggtac cactgaagtt
     9481 gctgttatct ccttgaacgg cgtggtttac tcctcttccg tacgcattgg tggtgaccgt
     9541 ttcgacgaag ctatcatcaa ctatgtgcgt cgtaattacg gttctctgat cggtgaagcc
     9601 accgcagaac gtatcaagca cgaaatcggt tcggcttatc cgggcgatga agtccgtgaa
     9661 atcgaagttc gtggccgtaa cctggcagaa ggtgttccac gcggttttac cctgaactcc
     9721 aacgaaatcc tcgaagcact gcaggaaccg ctgaccggta ttgtgagcgc ggtaatggtt
     9781 gcactggaac agtgcccgcc ggaactggct tccgacatct ccgagcgcgg catggtactc
     9841 accggtggtg gcgcactgct gcgtaacctt gaccgtttgt taatggaaga aaccggcatt
     9901 ccagtcgttg ttgctgaaga cccgctgacc tgtgtggcgc gcggtggcgg caaagcgctg
     9961 gaaatgatcg acatgcacgg cggcgacctg ttcagcgaag agtaatcgga tgcaggcagg
    10021 ggaagcgtct gtttaccctg cctggtctga tacgagaata cgcataactt atgaagccaa
    10081 tttttagccg tggcccgtcg ctacagattc gccttattct ggcggtgctg gtggcgctcg
    10141 gcattattat tgccgacagc cgcctgggga cgttcagtca aatccgtact tatatggata
    10201 ccgccgtcag tcctttctac tttgtttcca atgctcctcg tgaattgctg gatggcgtat
    10261 cgcagacgct ggcctcgcgt gaccaactag aacttgaaaa tcgggcgtta cgtcaggaac
    10321 tgttgctgaa aaacagtgaa ctgctgatgc ttggacaata caaacaggag aacgcgcgtc
    10381 tgcgcgagct gctgggttcc ccgctgcgtc aggatgagca gaaaatggtg actcaggtta
    10441 tctccacggt taacgatcct tatagcgatc aagttgttat cgataaaggt agcgttaatg
    10501 gcgtttatga aggccagccg gtcatcagcg acaaaggtgt tgttggtcag gtggtggccg
    10561 tcgctaaact gaccagtcgc gtgctgctga tttgtgatgc gacccacgcg ctgccaatcc
    10621 aggtgctgcg caacgatatc cgcgtaattg cagccggtaa cggttgtacg gatgatttgc
    10681 agcttgagca tctgccagcg aatacggata ttcgtgttgg tgatgtgctg gtgacctccg
    10741 gtctgggcgg tcgtttcccg gaaggctatc cggtcgcggt tgtctcttcc gtaaaactcg
    10801 atacccagcg cgcttatact gtgattcagg cgcgtccgac tgcagggctg caacgtttgc
    10861 gttatctgct gctgctgtgg ggggcagatc gtaacggcgc taacccgatg acgccggaag
    10921 aggtgcatcg tgttgctaat gaacgtctga tgcagatgat gccgcaggta ttgccttcgc
    10981 cagacgcgat ggggccaaag ttacctgaac cggcaacggg gatcgctcag ccgactccgc
    11041 agcaacccac gacaggaaat gcagctactg cgcctgctgc gcccacacag cccgctgcta
    11101 atcgctctct acaaagggct acgccgccgc aaagtggtgc tcaaccgcct gcgcgtgcgc
    11161 cgggagggca atagtggcga gctatcgtag ccagggacgc tgggtaatct ggctctcttt
    11221 cctcattgcg cttttgctgc aaatcatgcc ctggccggat aacctgattg ttttccggcc
    11281 aaactgggtg ttactcatct tgttgtattg gatcctggcc ttgccccatc gcgtaaatgt
    11341 gggcacaggt tttgtgatgg gtgccatact ggatctgatc agcggctcga cgcttggcgt
    11401 acgcgtattg gcgatgagca tcattgctta cctggtggcg ctgaaatacc agcttttccg
    11461 caacctcgca ttatggcagc aggcgctggt cgtcatgttg ctttcgctgg tggtggatat
    11521 tattgttttc tgggcagagt ttttagtgat taacgtctct ttcagaccgg aagtgttctg
    11581 gagtagtgta gtcaatgggg tgctctggcc gtggattttc ttgctgatgc gtaaagtccg
    11641 tcagcagttt gcagtgcaat aaaggtttct atgacttctc tgtatttagc ttccggttct
    11701 ccgcgtcgtc aggagttact tgcgcaactt ggcgtgacct ttgaacgtat tgttacgggc
    11761 attgaggagc agcgtcagcc gcaggagagc gcgcagcagt atgttgtgcg tctggcgcgc
    11821 gagaaagcac gggcaggtgt cgcgcaaacg gcgaaggatc tcccggtgct gggtgcggat
    11881 actatcgtta tcctgaacgg agaagtgctg gagaaaccgc gcgacgcaga gcatgcggcg
    11941 cagatgttgc gcaaattatc gggtcagacc catcaggtga tgacggcagt ggcgttggcc
    12001 gacagccggc acattctcga ttgcctggtg gtcaccgatg tgactttcag aacgttaaca
    12061 gacgaagaca tcgcgggcta tgtcgccagc ggtgaaccgt tagataaagc aggtgcatac
    12121 ggtattcagg ggctgggtgg ctgttttgtc aggaagataa atggcagcta tcaagccgta
    12181 gtcggcttac cgctggttga aacgtatgaa ttattaagta attttaacgc actgcgtgag
    12241 aaaagggata aacatgacgg ctgaattgtt agtaaacgta acgccttcgg aaacgcgagt
    12301 ggcgtatatt gatggcggta ttctgcagga aattcatatt gaacgtgagg cgcgacgcgg
    12361 aatagtaggc aatatctaca agggtcgtgt aagtcgtgta cttccgggta tgcaggcggc
    12421 ttttgtagat attgggctgg ataaagccgc gtttcttcat gcatctgaca tcatgccgca
    12481 caccgaatgt gtggcgggtg aagaacaaaa gcaattcacg gtgcgcgaca tctcggaact
    12541 ggttcgtcag gggcaagatc tgatggtgca ggtggtgaaa gatccgcttg gcactaaggg
    12601 ggcgcgcctg accaccgata tcacgctgcc ttctcgctat ctggtcttta tgccaggggc
    12661 ttctcacgtt ggggtttccc aacgaattga aagcgaatca gaacgtgaac gcctgaaaaa
    12721 agtggtcgca gagtattgcg acgagcaggg cgggtttatc atccgtaccg cagcggaagg
    12781 ggttggcgag gctgaactgg cctccgatgc cgcttatctg aaacgcgtct ggaccaaagt
    12841 tatggagcgt aaaaagcgcc cgcagacccg ttatcagctg tacggcgaac tggcgttggc
    12901 gcagcgtgtt ctgcgtgatt tcgccgatgc cgaactggac cgcattcgcg ttgactcacg
    12961 cctgacttac gaagcgttac ttgagttcac ctcggagtac attcccgaga tgaccagcaa
    13021 gctggagcat tacaccggac gccagccgat tttcgatctc tttgatgtcg aaaacgaaat
    13081 ccagcgagcg ctggaacgca aagttgaact gaaatccggt ggttatctga ttatcgacca
    13141 gaccgaagcg atgaccaccg tggacatcaa taccggagcg tttgtcggtc atcgcaatct
    13201 ggacgacacc attttcaata ccaatattga agcgacgcag gctatcgctc gccagttacg
    13261 gttgcgtaat ctgggcggga ttatcattat tgatttcatc gatatgaata atgaagatca
    13321 ccgccgccga gtgctgcact cgctggagca ggcgttgagc aaagaccggg tgaaaaccag
    13381 cgttaatggt ttttcggcgc tggggctggt ggagatgacg cgtaaacgca cccgcgaaag
    13441 cattgagcac gtactgtgta acgaatgccc aacctgccac ggtcgcggaa cggtgaaaac
    13501 cgtggaaacg gtatgctatg aaatcatgcg cgagattgtt cgtgtacacc atgcttacga
    13561 ctccgaccgt ttcctggtct atgcttctcc ggcagtagcc gaagccttga aaggcgaaga
    13621 gtcacactcg ctggcggaag tggaaatttt cgttggcaaa caggttaaag tacaaattga
    13681 accgctctat aaccaggagc agtttgacgt cgtaatgatg taaacagatg ctgggccgcc
    13741 atccggcaaa gggtttttga gtcacatttt tagcagacaa ggagtgacgg gtgaggcgat
    13801 tgccggggat tttactgctt actggagccg cgctcgttgt gatcgctgcc ctgctggtta
    13861 gcggcctgcg tattgcttta ccgcatcttg acgcctggcg tccggaaatc ctcaacaaaa
    13921 tagaatccgc gactggcatg ccggtagaag ccagtcagct ctcagccagc tggcagaatt
    13981 ttggcccgac gcttgaagcg cacgacatcc gtgcagaact aaaggatggc ggcgaatttt
    14041 cggttaaacg cgttactctg gcgctggatg tctggcagag cctgttacat atgcgctggc
    14101 agtttcgcga cctcactttc tggcagctgc gctttcgcac caacactcct atcaccagcg
    14161 gtggtagcga tgacagtctg gaagccagtc acatcagcga tctgtttctt cgtcaatttg
    14221 accatttcga tctccgcgac agtgaagtca gtttcctgac gccatccggt cagcgcgccg
    14281 agctggcgat cccacaactc acctggctga acgatccacg tcgacaccgt gcggaaggcc
    14341 tggtaagcct ctccagcctt accggacagc acggcgtgat gcaggtgcgc atggatttgc
    14401 gcgatgatga ggggttgtta agcaatggtc gcgtctggct ccaggcggat gacatcgacc
    14461 tgaagccgtg gctcggtaaa tggatgcagg acaatattgc gctggaaacg gcgcagttct
    14521 cccttgaagg ctggatgacg atcgacaaag gcgatgtaac cggcggtgac gtctggctga
    14581 aacagggtgg tgccagctgg ttgggcgaga aggaaacgca tacgctgtcg gtggataatc
    14641 tgaccgcgca tattacgcgt gaaaatccgg gatggcagtt ctctattccc gatacacgga
    14701 tcacgatgga cggcaaaccc tggccgagcg gagcattgac gctggcctgg ataccggaac
    14761 aggacgttgg cggcaaagac aataaacgca gtgacgaact ccggattcgc gccagtaatc
    14821 tggagctggc aggcctggag ggcatacgcc cgctggccgc gaaactttca cctgcactgg
    14881 gtgatgtttg gcgctctaca caaccgagcg gcaagattaa cactctggcg ctggatatcc
    14941 cgcttcaggc ggcagacaag acccgttttc aggcatcgtg gagcgatctg gcctggaagc
    15001 aatggaaatt attaccgggt gcggaacact tctccgggac gctttccggc agcgttgaaa
    15061 atggtttgct taccgcgtcg atgaagcagg caaagatgcc ttacgaaacg gtattccgtg
    15121 cgccactgga aatcgccgac ggccaggcaa ctataagctg gctgaacaat gacaaaggtt
    15181 tccagctgga tgggcgtaat attgacgtta aagccaaagc cgtccatgcg cgcggcggtt
    15241 ttcgttacct gcaacctgct aacgatgaac cctggctggg tattctggct ggcatcagta
    15301 ccgatgatgg ttcacaagcc tggcgctatt tcccggaaaa cttgatgggt aaagacctgg
    15361 ttgattactt aagtggcgcg attcagggcg gtgaagcgga taacgcgacg ctggtttatg
    15421 gtggcaatcc gcaactcttc ccctataaac acaacgaagg tcagtttgaa gtgctggtgc
    15481 cgctgcgcaa cgcgaagttt gccttccagc cggactggcc tgcattaact aaccttggta
    15541 ttgaactgga ctttattaac gacggtttat ggatgaaaac cgatggcgtt aatctgggcg
    15601 gcgtgcgcgc gagtaatctc accgcagtga tccctgacta ctcaaaagaa aaactgctga
    15661 ttgacgctga cattaaaggt ccgggtaaag ccgttggccc ttactttgat gagacaccgc
    15721 tgaaagattc tctgggtgcg accctgcaag aactccagct cgacggcgat gtgaatgctc
    15781 gcttacatct tgatatcccg ctgaacggcg agctggtaac cgcgaaaggt gaagtgacgc
    15841 tgcgtaataa cagtctgttt atcaaaccac tcgacagcac cctgaaaaat ttgagcggta
    15901 aattcagctt tatcaatggc gatctgcaaa gtgaaccact gacagcaagc tggtttaatc
    15961 agccgttgaa cgtggatttt tccaccaaag aaggggcaaa agcctaccag gtcgcggtga
    16021 atctcaacgg taactggcaa ccggcgaaaa ccggcgttct gcctgcagcg gtgaacgaag
    16081 cattgagtgg cagcgtggcg tgggatggta aagtgggcat tgttctgcct tatcatgctg
    16141 gcgcgacgta taacgtagag ctaaacggcg atttgaagaa tgtgagcagt cacttacctt
    16201 caccgttagc caaacctgcg ggtgaaccac tgccggtaaa cgttaaggtt gatggcaatc
    16261 tcaacagctt tgaattaacc ggacaggctg gtgcggataa tcatttcaat agccgctggt
    16321 tgctcggtca aaagctgacg ctcgaccgtg ctatttgggc ggcagacagt aaaacgctcc
    16381 cgccgttgcc ggaacaaagt ggtgttgaac tcaatatgcc gccgatgaat ggtgccgagt
    16441 ggctggccct gtttcagaaa ggtgcggcgg agagtgtcgg tggtgcagcg agtttcccac
    16501 aacacataac gttacgtacg cctatgttgt cgctgggaaa tcagcaatgg aataacctga
    16561 gtattgtttc gcaaccgacg gcaaatggca cccaggttga ggcgcagggg cgtgaaatca
    16621 acgccacgct ggcgatgcgt aataacgcgc cgtggctggc gaatatcaaa tatctttatt
    16681 acaacccgag cgtggcgaaa actcgtggtg attcaacgcc gtcatcacct ttcccgacaa
    16741 cggaacgcat taacttccgt ggctggtcgg acgcccaaat acgatgcgca gagtgctggt
    16801 tctgggggca aaaattcggg cgcattgaca gtgatatcac catttctggc aatacattaa
    16861 cgctgaccaa tggactgatt gatactggtt tttcgcggct cactgccgat ggtgaatgga
    16921 ttaacaatcc ggggaatgaa cgtacctcgc tgaaaggaaa actgcgcggg cagaaaattg
    16981 atgccgccgc agaatttttt ggtgtcacga cgcccatacg gcagtcgtca tttaatgtgg
    17041 attacgattt acactggcgt aaagcaccgt ggcagccaga tgaggcgacg ttgaatggca
    17101 tcattcatac tcaactgggt aaaggcgaaa ttaccgaaat caataccgga catgccgggc
    17161 aattgctgcg cttattgagc gtagatgccc tgatgcgtaa gctgcgtttt gatttcagag
    17221 acacttttgg cgaagggttc tattttgact ccattcgcag caccgcgtgg attaaagacg
    17281 gcgttatgca caccgacgac acgctggtgg atggcctgga ggcggatatc gccatgaaag
    17341 ggtcggtaaa tctggtacgt cgcgacctga atatggaagc ggttgtcgca ccagagattt
    17401 ctgcgacagt gggcgtggct gcggcttttg cggttaaccc cattgttggc gcggcagtgt
    17461 ttgccgccag taaagtgctg gggccgctgt ggagcaaagt ctccattttg cgctatcaca
    17521 tttcgggtcc gctggacgat ccgcaaatca acgaagtgtt gcgccaaccg cgtaaagaaa
    17581 aagcgcaatg atttgacgag ggcgcgtaat tgccccaatc tcataggata atcgttgcca
    17641 aaggccaacg agccagaaca taaccgtagg ctggataggg cgttcacgcc gcatccggca
    17701 gccgtaaaaa atcctctact gcagtaacta acgagtagca aaaacgatga gtcttaacct
    17761 ggtaagtgaa caattgctag cggcgaacgg cctgaaacat caggacttgt tcgtgatcct
    17821 cggtcaactg gccgaacgtc gccttgatta tggcgatctc tattttcagt cgagctatca
    17881 cgaatcctgg gttttagaag accgcattat taaagatggt tcttacaaca tcgatcaggg
    17941 cgttggtgtg cgtgcaatca gcggtgaaaa aaccggattt gcttacgctg accaaatcag
    18001 cctgctggcg ctggaacaga gtgcgcaagc ggcgcgcacc atcgcccgtg atagcggtga
    18061 tggcaaagta cagacgctgg gcgcggtaga gcatagcccg ttgtatacct cggtagatcc
    18121 gctgcaaagc atgagccgtg aagagaagct ggatatcctg cgtcgcgtcg ataaggttgc
    18181 ccgcgaagcg gacaagcgcg tacaggaagt gactgccagc ctcagtggtg tctatgaatt
    18241 aattctggtt gcggccaccg acggcacgct agcggcggat gtccgtccgc tggtgcgtct
    18301 ttccgtgagc gttctgatcg aagaagatgg caaacgcgaa cgcggtgcca gtggcggcgg
    18361 cggtcgtttt ggttatgagt tcttccttgc cgatctcgac ggcgaagtcc gtgcggatgc
    18421 atgggcaaaa gaagcagtac gtatggcgct ggtcaatctt tctgccgttg ctgcaccagc
    18481 gggcaccatg ccggtagtac ttggcgcagg ttggccgggc gtgctgttgc atgaagcggt
    18541 aggtcacggt ctggaaggcg acttcaaccg ccgtggcact tcagtattta gtggacaggt
    18601 cggggagctg gtggcttcag aactgtgtac cgtggttgat gacggtacga tggtcgatcg
    18661 ccgtggttcg gtggcgattg atgacgaagg tacgccaggc cagtacaacg tgctgattga
    18721 gaacggcatt ctgaaaggct acatgcagga taaactcaac gcgcgtttga tggggatgac
    18781 gccgactggc aacggtcgcc gtgaatccta cgcccatctg cccatgccgc gtatgaccaa
    18841 cacctatatg ctgccgggta aatcgacccc gcaggaaatt attgaatccg ttgagtacgg
    18901 tatctatgca ccgaactttg gtggcggtca ggtggatatc acctccggca aattcgtttt
    18961 ctccacttca gaagcatatc tgattgaaaa cggtaaagta acgaagccgg tgaaaggcgc
    19021 aacgttgatt ggttccggta tcgaaaccat gcagcagatt tcgatggttg gcaacgacct
    19081 gaaactggat aacggcgtgg gtgtctgcgg taaagaaggg caaagtttgc cggttggcgt
    19141 gggccagcca acgctgaaag ttgataacct gactgttggc ggtactgcgt aataatttca
    19201 gtattttcag aaggataatt aatccttcct cgtgcacgaa cggtcccctc gcccctttgg
    19261 ggagagggtt agggtgaggg gaacccgttg gcacaggttt gtacaacgta acagtacaat
    19321 atgaattact tctctttccc gcgcccgtgc atctcctgaa acaatttacc gacctcaaca
    19381 aaataatccg tcagcgagtt gatcacgacc tgtaccttca gcggcagctt atctttttcg
    19441 gtatataacg cataaaccgg gcgtggatct gactgataac gcggcagcag gatctccagc
    19501 tccccacgat tgatctcgtt gatcacccac atcagcggca cgtaggcgat cccggcaccc
    19561 gccgttagcc agcgcaccag cgtcatcgga tcattagtca caaatctccc ttgcgggatc
    19621 aggcgagtcg agatcccttc cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg
    19681 tattcaagcc atgaatggct acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg
    19741 agatagcttt tcgcggcgca caccaccatt ggcatcgcgc caagacggcg ggaaaacagg
    19801 ctggaatcct gcaacgcgcc gacgcggatc accacatcca gaccgtcggc aatcaggtcg
    19861 ggggctggaa ttccggtaac cagattgacg ctcaaacctg ggtattcttt cagcattttg
    19921 gctgtcagcc cggcgagaac attttgtgcc atagttgaag aacagccaat gcgtagcgtc
    19981 ccgatggggg tgttattgaa ggcatacagt tgctcatgaa catcctgcac ttcatgaagc
    20041 atacgacggc agccctggta gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg
    20101 ctacggttta acagctttac ctgcaactca tcttccagtt ttgacaccgt ctgactgatg
    20161 gacgaaacgc tcatctgtag ctgtctggcg gcggcggtaa aagagccaaa ttcaactact
    20221 ttggcaaaca ccgacatgcg ttttagtcgt tccattattc actctgactt aaaagtgatt
    20281 tagatcacat aatatagata acagcataac agttacgcta atatattaaa tatcaatcta
    20341 cagcaatgtt gctctcgccc ggctttccat gcccttctct gcggcgacag atgctgaaaa
    20401 taataacgcc tgctctctct atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt
    20461 ggtgtttggg ctgtccttcc caccgatatt ttttgaattg cttttatcac tggcgatttt
    20521 ctggctggtg tgccgggtac ttgtgccaac aggtatctac gactttgtct ggcatccggc
    20581 gttgttcaac accgcgctct attgctgctt gttttatttg atatcgcgac tgttcgtttg
    20641 aggttgaagt gaaaacacta ataagaaaat tctcccgtac ggccatcacg gtcgtattag
    20701 tcattctggc cttcatcgca atttttaatg cctgggtcta ttacaccgaa tccccctgga
    20761 cgcgtgacgc gcgctttagc gctgacgtcg ttgcgatcgc gccggacgtt tctggactca
    20821 ttacccaggt gaatgttcat aaccagctgg tgaaaaaagg acaggttctg ttcaccatcg
    20881 accagccgcg ctatcaaaag gcgcttgagg aagcgcaagc cgatgttgct tattatcagg
    20941 tactggcaca ggagaaacgc caggaggccg gacgtcgtaa ccgtctcggt gtgcaggcga
    21001 tgtctcgcga agagatcgac caggccaaca acgtactaca aacggttctg catcagttag
    21061 cgaaagcgca ggcgacccgc gatctggcaa aactggatct tgaacgcacg gtgatccgcg
    21121 cgccagcaga tggctgggtg accaacctca acgtctatac cggtgagttt attactcgtg
    21181 gatcaacggc ggttgcgctg gtgaaacaga actctttcta tgtactggcc tatatggaag
    21241 aaactaagct ggaaggggtg cgccctgggt atcgtgcaga gatcacgccg cttggcagta
    21301 acaaagtgct gaaagggact gttgatagtg ttgccgctgg ggtcaccaac gccagcagca
    21361 cgcgtgacga caaagggatg gcgaccatcg actctaacct tgaatgggtg cgtttggcgc
    21421 aacgtgttcc ggttcgtatt cgtctcgaca accagcaaga gaacatctgg cctgcgggca
    21481 ccactgctac agtggtggtc actggcaaac aagatcgcga cgaaagccag gattcgttct
    21541 tccgtaaaat ggcccatcgc ctgcgtgagt ttggttaatc acgatggata ttttctccat
    21601 tgctaaccaa catattcgct ttgcggtaaa actggcgacc gccattgtac tggcgctgtt
    21661 tgttggcttt cacttccagc tggaaacgcc acgctgggcg gtactgacag cggcgattgt
    21721 tgccgccggt ccggcctttg ctgcgggagg tgaaccgtat tctggcgcta ttcgctatcg
    21781 tggctttttg cgcatcatcg gcacatttat tggctgtatt gccggactgg tgatcatcat
    21841 tgcgatgatc cgcgcaccat tattgatgat tctggtgtgc tgtatctggg ccggtttttg
    21901 tacctggata tcctcgctgg tacgaataga aaactcgtat gcgtgggggc tggccggtta
    21961 taccgcgctg atcattgtga tcaccattca gccggaacca ttgcttacgc cgcagtttgc
    22021 cgtcgaacgt tgtagcgaga tcgttatcgg tattgtgtgt gcgattatgg cggatttgct
    22081 cttttctccg cgatcgatca aacaagaagt ggatcgagag ctggaaagtt tgctggtcgc
    22141 gcaatatcaa ttaatgcaac tctgtatcaa gcatggcgat ggtgaagttg tcgataaagc
    22201 ctggggcgac ctggtgcgac gcaccacggc gctacaaggc atgcgcagca acctgaatat
    22261 ggaatcttcc cgctgggcgc gggccaatcg acgtttaaaa gcgatcaata cgctatcgct
    22321 gacgctgatt acccaatcct gcgaaactta tcttattcag aatacgcgcc cggaattgat
    22381 cactgatact ttccgcgaat tttttgacac gccggtagaa accgcgcagg acgtccacaa
    22441 gcagctcaaa cgcctgcgaa gagttatcgc ctggaccggg gaacgggaaa cgcctgtcac
    22501 catttatagc tgggtcgcgg cggcaacgcg ttatcagctt ctcaagcgcg gcgttatcag
    22561 taacacaaaa atcaacgcca ccgaagaaga gatcctgcaa ggcgaaccgg aagtcaaagt
    22621 agagtcagcc gaacgtcatc atgcgatggt taacttctgg cgaaccacac tttcctgcat
    22681 tctgggaacg cttttctggc tgtggacggg ctggacttcc ggcagtggtg caatggtgat
    22741 gattgcggtc gtgacgtcac tggcaatgcg tttgccgaat ccacgcatgg tggcgatcga
    22801 ctttatctac gggacgctgg ccgcgctgcc gttagggctg ctctactttt tggtgattat
    22861 ccctaatacc caacagagca tgttgctgct gtgtattagc ctggcagtgc tgggattctt
    22921 cctcggaata gaagtacaga aacggcgact gggctcgatg ggggcgctgg ccagcaccat
    22981 aaatattatc gtgctggata acccgatgac tttccatttc agtcagtttc tcgacagcgc
    23041 attagggcaa atcgtcggct gtgtgctcgc gttcaccgtt attttgctgg tgcgggataa
    23101 atcgcgcgac aggactggac gtgtactgct taatcagttt gtttctgccg ctgtttccgc
    23161 gatgactacc aatgtggcac gtcgtaaaga gaaccacctc ccggcacttt atcagcagct
    23221 gtttttgctg atgaataagt tcccagggga tttgccgaaa tttcgcctgg cgctgacgat
    23281 gattatcgcc caccagcgcc tgcgtgatgc gccgatcccg gttaacgagg atttatcggc
    23341 gtttcaccga caaatgcgcc gcacagcaga ccatgtgata tctgcccgta gcgatgataa
    23401 acgtcgtcgg tactttggtc agttgctgga agaactggaa atctaccagg aaaagctatg
    23461 catctggcaa gcgccaccgc aggtgacgga accggttcat cggctggcgg ggatgctcca
    23521 taagtatcaa catgcgttga ccgatagtta agtcaaaacc gacgccaaaa gcgtcggttt
    23581 tttcatggct atacttagcg atgcacggca gaactcgccg cgaaacgtga cggtggcaac
    23641 agatgaatat ttataccttt gattttgatg agattgagag tcaggaggat ttttatcgtg
    23701 actttagcca aacctttggt ctggcgaaag ataaggtacg cgatctcgac tcactatggg
    23761 atgtgttaat gaacgatgtc ctgccgctac cacttgagat tgaatttgtt catctgggag
    23821 agaaaacgcg tcgccgtttt ggcgcgttaa tattgctgtt tgatgaggca gaggaagagc
    23881 tggaagggca tttgcgtttt aatgttcgtc attagcgcaa aaaaaagccc ccgaaccggg
    23941 ggcaatatcg tcggacaaga cgatgagggt ttatttgtac agttcagccg tagcgtgcca
    24001 ggtgtcaccg ctacgagctt cagtaatctg gtaggccgtt gcgcctttct cttccgcttt
    24061 tttgttcagc atttcacgca tatccattgg cgaagacgcc acaccactta cggatacggt
    24121 cccgattgct tcacgatttt gtgcttgtgc agcatcaatg gagtcggcag cgaatgcacc
    24181 gaaagagaga acagaaagta cgcttaatgc agcaacagtg gttttgattt tcatgatttt
    24241 tacctcgaca taatctttta gctgggcctt tgtttcgtga cccatatcac aaaatcaagt
    24301 atacacta
//
LOCUS       DAZKIF010000070        24218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZKIF010000070 DAZKIF010000000
VERSION     DAZKIF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.070"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..383
                     /locus_tag="ACHOTW_002744"
     CDS             <1..383
                     /locus_tag="ACHOTW_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9844821.1"
                     /translation="LKSLESWLREKMKTLSRHLELAKAFAYALNQWPALTYYANDGWV
                     EIDNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGMCKLNDVDPESYLRHVL
                     GVIADWPVNRVSELLPWRIALPAE"
     gene            complement(500..1765)
                     /locus_tag="ACHOTW_002745"
     CDS             complement(500..1765)
                     /locus_tag="ACHOTW_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9844822.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1963..2038)
                     /locus_tag="ACHOTW_002746"
     tRNA            complement(1963..2038)
                     /locus_tag="ACHOTW_002746"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2003..2005),aa:Phe,seq:gaa)
     gene            complement(2144..2851)
                     /gene="yqgA"
                     /locus_tag="ACHOTW_002747"
     CDS             complement(2144..2851)
                     /gene="yqgA"
                     /locus_tag="ACHOTW_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9844823.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3004..3087
                     /locus_tag="ACHOTW_002748"
     CDS             3004..3087
                     /locus_tag="ACHOTW_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844824.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            3249..5384
                     /gene="speC"
                     /locus_tag="ACHOTW_002749"
     CDS             3249..5384
                     /gene="speC"
                     /locus_tag="ACHOTW_002749"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP9844825.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5434..6690)
                     /gene="nupG"
                     /locus_tag="ACHOTW_002750"
     CDS             complement(5434..6690)
                     /gene="nupG"
                     /locus_tag="ACHOTW_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP9844826.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6892..7971)
                     /gene="mltC"
                     /locus_tag="ACHOTW_002751"
     CDS             complement(6892..7971)
                     /gene="mltC"
                     /locus_tag="ACHOTW_002751"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP9844827.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(8036..8311)
                     /gene="yggX"
                     /locus_tag="ACHOTW_002752"
     CDS             complement(8036..8311)
                     /gene="yggX"
                     /locus_tag="ACHOTW_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP9844828.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8339..9391)
                     /gene="mutY"
                     /locus_tag="ACHOTW_002753"
     CDS             complement(8339..9391)
                     /gene="mutY"
                     /locus_tag="ACHOTW_002753"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9844829.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9552..10271
                     /gene="trmB"
                     /locus_tag="ACHOTW_002754"
     CDS             9552..10271
                     /gene="trmB"
                     /locus_tag="ACHOTW_002754"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9844830.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10271..10597
                     /gene="yggL"
                     /locus_tag="ACHOTW_002755"
     CDS             10271..10597
                     /gene="yggL"
                     /locus_tag="ACHOTW_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP9844831.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10781..11500
                     /gene="yggN"
                     /locus_tag="ACHOTW_002756"
     CDS             10781..11500
                     /gene="yggN"
                     /locus_tag="ACHOTW_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP9844832.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11676..12722
                     /gene="ansB"
                     /locus_tag="ACHOTW_002757"
     CDS             11676..12722
                     /gene="ansB"
                     /locus_tag="ACHOTW_002757"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP9844833.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12839..13846
                     /gene="yggM"
                     /locus_tag="ACHOTW_002758"
     CDS             12839..13846
                     /gene="yggM"
                     /locus_tag="ACHOTW_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP9844834.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQATASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13998..15134)
                     /gene="hemW"
                     /locus_tag="ACHOTW_002759"
     CDS             complement(13998..15134)
                     /gene="hemW"
                     /locus_tag="ACHOTW_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9844835.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(15127..15720)
                     /gene="rdgB"
                     /locus_tag="ACHOTW_002760"
     CDS             complement(15127..15720)
                     /gene="rdgB"
                     /locus_tag="ACHOTW_002760"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP9844836.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15728..16018)
                     /gene="yggU"
                     /locus_tag="ACHOTW_002761"
     CDS             complement(15728..16018)
                     /gene="yggU"
                     /locus_tag="ACHOTW_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP9844837.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(16015..16581)
                     /gene="yggT"
                     /locus_tag="ACHOTW_002762"
     CDS             complement(16015..16581)
                     /gene="yggT"
                     /locus_tag="ACHOTW_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP9844838.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16599..17303)
                     /gene="yggS"
                     /locus_tag="ACHOTW_002763"
     CDS             complement(16599..17303)
                     /gene="yggS"
                     /locus_tag="ACHOTW_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP9844839.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17321..18301
                     /gene="yggR"
                     /locus_tag="ACHOTW_002764"
     CDS             17321..18301
                     /gene="yggR"
                     /locus_tag="ACHOTW_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP9844840.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18492..18908)
                     /gene="ruvX"
                     /locus_tag="ACHOTW_002765"
     CDS             complement(18492..18908)
                     /gene="ruvX"
                     /locus_tag="ACHOTW_002765"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9844841.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18908..19471)
                     /gene="yqgE"
                     /locus_tag="ACHOTW_002766"
     CDS             complement(18908..19471)
                     /gene="yqgE"
                     /locus_tag="ACHOTW_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP9844842.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19583..20530)
                     /gene="gshB"
                     /locus_tag="ACHOTW_002767"
     CDS             complement(19583..20530)
                     /gene="gshB"
                     /locus_tag="ACHOTW_002767"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP9844843.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20543..21274)
                     /gene="rsmE"
                     /locus_tag="ACHOTW_002768"
     CDS             complement(20543..21274)
                     /gene="rsmE"
                     /locus_tag="ACHOTW_002768"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9844844.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21354..22061)
                     /gene="endA"
                     /locus_tag="ACHOTW_002769"
     CDS             complement(21354..22061)
                     /gene="endA"
                     /locus_tag="ACHOTW_002769"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP9844845.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(22156..22653)
                     /gene="yggI"
                     /locus_tag="ACHOTW_002770"
     CDS             complement(22156..22653)
                     /gene="yggI"
                     /locus_tag="ACHOTW_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP9844846.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22730..24124)
                     /gene="galP"
                     /locus_tag="ACHOTW_002771"
     CDS             complement(22730..24124)
                     /gene="galP"
                     /locus_tag="ACHOTW_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP9844847.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5959 a         6093 c         6362 g         5804 t
ORIGIN      
        1 tgttgaaatc cctggaaagc tggttgcgtg aaaagatgaa gaccctgtcg cgacacttag
       61 agttggcgaa ggcgttcgcg tacgcactta accagtggcc ggcactgacg tactatgcga
      121 acgatggctg ggtggaaatc gacaacaaca tcgctgaaaa tgccctgcgg gcggtcagtc
      181 tgggtcgtaa aaacttcctg ttcttcggct ctgaccatgg tggtgagcgg ggagcgctac
      241 tgtacagcct gatcgggatg tgcaaactga atgacgtgga tccagaaagc taccttcgcc
      301 atgtgcttgg cgtcatagca gactggccgg tcaaccgggt cagcgaactg cttccgtggc
      361 gcatagcact gccagctgaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac
      421 gaagctaata ccgttttgtc tgttttttta acaaagaaaa taatcctgga gtaggttccc
      481 gggcatttgc ccgggatgat tactgtttta atggattatt aatctttgca tattcaaatg
      541 gactgataaa tctttctctg tttgcatcca gaaaatcggc ccaccactgt agcatcagtc
      601 gccgttcttc cagatgttct gctttatgaa tgtaagcagc acgtacaccg ttacgttcct
      661 gatggctcat ctgacgttca actgcatcgc gggaccacaa acctgattct attaacgcac
      721 tacatgccat cgcccggaat ccatgaccac agacatcctg ggttgtgtca taccccataa
      781 ccctcagggc tttatttacg gtgttttcac tgattggttt acgtgggtca tgtgctcccg
      841 taaagatcag accattttca ccagcccatg tctgaagttc tgccagtatg gctacaacct
      901 gtttgctgag tggcacaaga tgtggtgttc gcatcttgga tccccggtgt gagaatttta
      961 cgcctggcag aggttcgcgt tcggctggaa tagtccacat ggcattgtca atatcaattt
     1021 cagaccagcg ggcgtatctg agttcactgg aacgaataaa aaccagtaaa ttcagcataa
     1081 cggcaagctg ggtgacaggt tgccccttgt aactgccaat acgcgacaaa agttcaggca
     1141 ggcgagaaag atcaagagcg gggcgatgct ggcgttttac cgtagtcaat gcgccagcca
     1201 tatccagagc aggattatat ctgatgatgc cagactgtac ggcataacgc attacggcag
     1261 atattctttg ctgcagacga ctggcgattt cataaatttg tttagcttct gcagcacgaa
     1321 cagggataag caggtccggg gtattgagtg ttgtgatatc ccgattgccg ataaagggga
     1381 atacgtgtgt ctcgaggctt ttgagtactt tatcgctgtg tgattgtgcc cattgtttat
     1441 tactggcatg ccagcgtcgt gcaatatgtt cgaaagagag ttgtaaatca gggactactt
     1501 ttttagctct tttctgttcg caagggtcaa ccccctccga aatcagtttt cgggcctcat
     1561 cccgttttgt acgagcttcg gagagagata cttctggata cacaacaagt gccagagtct
     1621 tctctttacc cagaatacga taacggagac gccagtaacg ggaaccatta ggatgtacca
     1681 gcaggaacat gccagccccg tcagtgagtt tataagcctt gtcagtgggc tttgcagccc
     1741 ggatttttgc gtcagtcagt gccatagttc agccttctga atgcgttaaa gagggtacaa
     1801 attctgtcga actggggtat gcccgcaatt atacccacac aatgaggttg atgtggggag
     1861 aatttggttg agttcggtag aattgatttg gaggaagaac acttaaatca tggtgttctg
     1921 aaacgaaaaa cggacctccg tggaggtccg ttgatatgaa tttggtgccc ggactcggaa
     1981 tcgaaccaag gacacgggga ttttcaatcc cctgctctac cgactgagct atccgggcaa
     2041 cggggcgcat taaacctgat tcgcctcgtc tcgtcaatca aatttcttca atttactgca
     2101 gactgcacaa tctatcatca ctttgccgct attgcacaca ttgtcaggca aaccaggcgg
     2161 tccaggcggc ggaaagcggc attgccagaa ggagtgctgg aagcatgttg accaccggga
     2221 acattttaat gccacagatg cgtaatccgg ttgccagcag caataaaccg cctacggcgc
     2281 tgaagtctgc catcatcgac ggtgtggtca gcggtaatat cagcgcggca gcccacgcca
     2341 gcgttaactg gatgatcagt aatgggatac taatcaccga taccgcaata cccagcgagc
     2401 aggcgaagat catcgccgta aagaaatcaa gaaatgactt ggcgattaaa atactcggat
     2461 cgccggtcat cccttcgttc atcgccccga agatcccggt gccgctggcg caaaacagga
     2521 caataatcgc gacataattc tgaataaaag attcatgcgc tggcttctta cgtgagtgac
     2581 gaaacagatt ttgtgctttg gtgaccgctg tattgacgcc tttctccagc agacaaattt
     2641 cgccgattaa tgctccgagt aaggttgcta aaaccatcgc cggaaggttg gcgcatttca
     2701 ccaccagtaa aataccaatc cccagcgatg ccaaaccaaa aattgatgtc atggagacgc
     2761 ggatacgttc cggtaagcgt tggctaagca gtgcaccgag catgccaccc agtaatattg
     2821 cacttgcgtt gataaaaggg ccgatgacca caagagttcc tgttaactgt attgtttgat
     2881 tttcttatta tgaacttttt gccttgttga tggcttgtct ggtcgcaaag tcctggcttg
     2941 cacgccttag cgaaaggtgc catgattgcg cgaattttct cctctctgta cggagtttgc
     3001 ccgatgcacg ccatctcctt acattctctc gcttatcgcc gtttcgcgcg aaacccttct
     3061 ctttttatgc tactgctcat gcggtgaagt taacgcacgc tcactgcagg acaacagtaa
     3121 aatcagagcc tttctgcttt tactgatgtc tggcggtcgg agctggtgac cagtttgacc
     3181 catatctcat ggggcagggt tttacacctt gtccagtatt cttacttccc cgaaacgggt
     3241 ttgcgcttat gaaatcaatg aatattgccg ccagtagtga actggtatcc cgactttcta
     3301 ctcatcgccg cgtggtggcg ttgggagata ctgattttac ggacgtcgcg gcagtcgtca
     3361 ttaccgctgc ggatagtcgc agtggcattc ttacgttgct taagcgcacc ggttttcatc
     3421 taccggtgtt tttgtattcc gaacatgctg ttgaattacc tgcgggcgtt acggcggtaa
     3481 tcaacggcaa cgagcagcag tggctggagc tggaatccgc agcctgtcag tatgaagaga
     3541 atttgctgcc accgttttat gacacgctga cgcagtacgt tgagatgggc aacagcacct
     3601 ttgcttgccc tggacatcaa catggtgcgt tctttaaaaa gcatcctgcc ggacgccatt
     3661 tttacgattt ctttggtgag aacatctttc gcgccgatat gtgtaacgct gacgtaaaat
     3721 tgggcgatct gcttattcat gaaggatcgg cgaaagatgc gcagaaattc gcagccaaag
     3781 tctttcatgc cgataaaacc tattttgtgc tgaacggcac atcggcagcg aataaagtgg
     3841 tgacgaatgc gctgttaacg cgtggcgatc tggtgctctt tgaccgtaac aaccataagt
     3901 cgaatcatca cggcgcgttg attcaggcgg gggcgacgcc ggtctatctg gaagcttcac
     3961 gtaacccgtt tggtttcatt ggcggtattg atgcgcactg ttttaatgaa gagtatctgc
     4021 gccagcaaat tcgcgacgtt gcgccagaaa aagccgacct gccgcgcccg tttcgcctgg
     4081 cgattattca gctgggaacc tatgacggca ctgtctataa cgcccgtcag gtgatcgata
     4141 ccgttgggca tctgtgtgat tacattctgt ttgattccgc gtgggtcggt tatgaacaat
     4201 ttatcccgat gatggcggat agctcgccgc tgctgttaga acttaacgaa aacgatccgg
     4261 ggatctttgt gactcagtcg gtgcacaaac agcaggcggg attctcacag acgtcgcaga
     4321 tccataaaaa agataaccat atccgcggac aggcgcgttt ttgcccgcat aagcggttga
     4381 ataacgcctt tatgctccat gcttctacca gccctttcta tccgctgttt gctgcactgg
     4441 atgttaacgc caaaattcat gaaggggaga gtgggcgtcg gctgtgggct gagtgcgttg
     4501 agttgggtat tgagtcgcgc aaggctattc ttgcgcgctg taagctgttc cgcccgttta
     4561 tcccgcccgt tgttgatggc aaattgtggc aggattatcc gacatcagtg ttagccagcg
     4621 accgtcgttt tttcagtttt gagccggggg cgaagtggca cggctttgaa ggatatgccg
     4681 cggatcagta ttttgttgat ccgtgcaagc tgttactcac tacaccaggt atcgatgccg
     4741 aaaccggcga atatagcgac tttggcgttc cggcgacgat tctggcgcac tatctgcgtg
     4801 agaacggcat tgtgccggag aagtgcgatc tcaattccat tctgttctta ttaactccgg
     4861 cggaaagcca cgagaagctg gcacaactgg tggcgatgct ggctcaattt gaacagcata
     4921 ttgaggatga ctcgccgctg gctgaggtgt tgccgagcgt ttataacaaa tatccggtgc
     4981 gctatcgcga ctacaccctg cgccagttgt gtcaggagat gcacgatctg tatgtcagtt
     5041 tcgacgtcaa agacctacaa aaagcgatgt tccgccagca gagtttcccg tcagtggtga
     5101 tgaaccccca ggatgcgcat agcgcttata ttcgcggtga cgtggagttg gtgcggattc
     5161 gtgatgccga agggcgaatt gcggcagaag gggcgttgcc ttatccacct ggcgtgcttt
     5221 gcgtggtacc cggggaagtc tggggtgggg cggttcaacg ttatttcctt gcactggaag
     5281 aaggggtgaa tttgttgccg ggattttcgc cggagctgca aggtgtttat agcgaaaccg
     5341 atgcggatgg catgaaacgg ttgtacggtt atgtgttgaa gtaagaataa aaaaaacggg
     5401 tcgccttctg gcgacccgtt tttctttgcg taattagtgg ctaaccgtct gtgtacctgt
     5461 cgggacacga acgtgtttat atttgaacat cgccatgaac gcgaaggcca gaaccacgga
     5521 gtaaccagcg aaaatcaacc atacggtctg ccagtcggta ataccgtttt gggtgtacat
     5581 ctcaacaact ttaccgctca cgatgccgcc gaggatacag ccgaagccgt tagtcatcat
     5641 caggaacatc ccttgtgcac tggcgcgaat tgccgggcta acttcttttt cgacaaacac
     5701 cgaaccagag atgttgaaga agtcgaacgc acagccgtaa acaatcatcg atagaaccag
     5761 cagtacggtg ccgaacggag tcgggtcgcc gtaagcaaac agcgcaaaac gcaggatcca
     5821 cgccacaata ctgatcatca ttacgttctt aataccgtag cggcttaaga agaacgggat
     5881 ggtcagaatg aacagggttt cagagatctg cgaaatcgac atgatgattg acgcatgctg
     5941 cacaataaag ctgctggcaa acatcggatc tttgtcgaag ctgtgcagga aggtattacc
     6001 gaacatgttg gtaatctgca gttccgcgcc cagcagcatt gagaagatga agaagattgc
     6061 catacgcttg tttttaaaca gcgcgaatgc atcgaggccc agcagggttg tccagctctg
     6121 attcgcttgc tgtttagcaa ccggaatatg cggcagagtc agggtaaaca gaaccagaat
     6181 ggcggaaagt gctgcgccaa tatacagctg catgtggctt aattcgaagc cagacaggct
     6241 caccacccac attgccatga taaagccgat ggtgccccag atacggattg gcgggaagtc
     6301 agtaacgata tccatcccgg cattttgcag gcgatagtaa gagatggtgt tgattaaccc
     6361 aagcgttggc atataagcaa acgagttaat caatatcaca aggaacatcg cctccggcgt
     6421 cgtgacctgt gccgccatga acagcgtgat agcgccaatg gtgtggcaaa tggcatatac
     6481 ccatttcgca cttaaccatt tgtcggccac aatccccagc agcgcaggca taaagaccgc
     6541 ggcgataccc agtgagctat aaactgcgcc aatagaagca ccgtcaaact tcagggtaac
     6601 aaacatatag gaaccgaggg tcgtcagcca acttccccac agacagaact gcagaaaaga
     6661 gaggattttc agctgcagct taagattcat gttaatttcc tcacatcgtg atgcggatga
     6721 gtgtttgaaa aagcacattt gaccgtgcga gatgatggga ttctatcaac ttcgcggact
     6781 ggttttttgt tacctgtggc aaataattgc aaacattcct aatttgcaaa acgaaattgt
     6841 gatatggata acatttttgc ccctgagcat cgtcaggggc ggttagtgga attatcggcg
     6901 gcggtaggat ttttgcgcgg tattcacttt ataaagataa cggcgagatt ccgcagaggg
     6961 atggcgggtc gtcagcgttt gataaacatc gcccggcgtc atggtgttaa taatattagc
     7021 cgcctgaatc ttatcattcg aaaagactcg cagcacgctg cctgcgccgc cgttataggc
     7081 ggtgatgacg gcataacgcc gggatgtagg gttatcaatt ccgccgagat aaacattgtt
     7141 cagcatcgcc agatacgcgg tgccggtatc aatattgctg gcaggatcaa acaagaaact
     7201 gcggctcggc gtgccggatt tcccctgcga gcggaacaca tctttcccgg cagtatgttg
     7261 taccacctgc attaacccca gcgcatcgga acggctgacc gcatacgggt taaaggaaga
     7321 ttcagtctgc ataattgcca gaatcagcga ctcatcaacg ccatatttcc gtgacgcctg
     7381 gcggaccatg ccgagatatt tgtgcgcacg tttatcaagg tggttcggca ccatgttaat
     7441 ggtgacgctg tagatgatac gcagcccgtt gctgcggctc ttcagacggt ttttcagcag
     7501 ataatccgcg aagttgcttg cgcgaccttc ccagcgaatc ggctgcccgg tgttgtccac
     7561 cacctgaccg taaaggaaag gttctttcga aattgtaata tcatcaacgt cggaatagag
     7621 atcgaccgaa ctcggatcgt cacccatcag caacgttttg ataattgccc ggcgcaaatg
     7681 cgcggcaggt tctgtcccgg cgatggtttc gatagtaatc gtaccgtcat cgaagttgat
     7741 gtggctgcgg gtctgatatt gatcggtgta tttcacgtag tccttaggac cggcgatcac
     7801 cacctctttg aatccccaga tgttctcaat attgtgggca aattgcccca tcagaatatc
     7861 aaaaccgttg gtgtctttga cccaggcttc gttataggta ccgccttttt tggtcgtcga
     7921 acaggagatg agcaacggcg caatcaaagc cagcgcgaga tattttttca tcattccggg
     7981 tgcgtgttgt gtttgttttt atgcaagctg ccggaggcgt gctccggcac tgtttttatt
     8041 ttttatcttc cggcgtatag ccctcgatat gcacctcttt accctcgaac aggaagttga
     8101 ccatctcctg ctccagcagc ttgcggtgct cggcattcat catgttgagt ttcttttcat
     8161 taatcagcat ggtttgtttg tgctgccact gcgcccaggc ttctttggag atctcgttat
     8221 agatgcgttt tcccagttcg ccggggtaca gctgaaaatc ctgaccttct gcttcacgtt
     8281 gcaggaaagt acaaaaaatc gttctgctca taaatcatcc tctttatcga ctcacgcgct
     8341 aaaccggcgc gccagtgcgt aactgctgta acaaacgctc cacgggagcc gccaggccaa
     8401 ctgacggcgg ttgcgctaag ttataccaga gcgcattgcc ttcatccatg cagccggtga
     8461 atgacgacac gggaagccac ataggcacaa tatctaagtg gaaatggctg aaggtatgcc
     8521 gaaacgcggt cagctgcgtc aggttatcgg cagcaatctg ccgttgcgcc agccactgcc
     8581 gcaaactttc ttcgtcggca aactgcggga aacagtataa accgccccac aatccgctcg
     8641 gcggacgctg cgccagcaat acttcatctt cgtgctgtaa caggagaaag tagccggtgc
     8701 gctccggcag cgtctgtttc ggttttttgc ccggataaag cgaccagcta ttgttggtgg
     8761 cggcaataca tccgttttgt agcggacaga gcgaacattt cggcttcgaa cgcgtgcaaa
     8821 tcatcgcgcc caaatccatc atcgcctgat taaaccgttc cacgccgacc gcgggcgtca
     8881 cctgctcgct taaactccat aatttattct cgacctcttt tttcccaggc cagccgctta
     8941 cagcatagca gcgcgccagc acccgtttga cgttaccgtc gagaatcgga aagtgcttac
     9001 ccagagaaag cgatagaatc gcgcctgcgg tggaacgccc gacgcccggc agtgctgcaa
     9061 cttcctcaaa ggtttccggg aatttaccgc cgtgtaaggt cgccacttgt tgtgccgctt
     9121 tatgcagatt gcgcgcgcgg gcgtaatagc caagccccgt ccacaagtgg agaacttcgt
     9181 cgagcggcgc attggcgaga tcggtcaccg tcgggaagcg cgccataaag cgttcaaaat
     9241 aggggataac ggtcgcaacc tgagtttgtt gcaacatcac ttctgagagc catactttgt
     9301 agggcgtctt gtcaatttgc cagggcagag tttttcgccc gtatttatcg taccagtcca
     9361 gaacctgggc tgaaaattgc gacgcttgca tggtcaccga attcactgtt gttgggggca
     9421 agattgcagc acagcgacgg cagggtgtaa accggaactt tccgcagcca cggccttatc
     9481 atgcttgcat ccggcaacta actttggata atgcccgttt tcagaacact ttcacaagcg
     9541 actaaatctt tatgaaaaac gacgtcattt caccggaatt tgatgaaaac ggccgcccac
     9601 tgcgccgtat ccgtagtttt gtgcgccgcc aggggcgact gaccaaaggc caggaacatg
     9661 cgctggaaaa ctactggccg gtgatgggcg ttgagttcag cgaagatatg ctggatttcc
     9721 ccgcgctttt tggccgtgaa gcgccggtga cgcttgagat tggttttggc atgggggcgt
     9781 cgctggtggc aatggctaaa gatcgccccg agcaggactt cctcggcatt gaagtgcatt
     9841 caccgggcgt tggtgcgtgc ctggcttctg cgcatgaaga gggcttaagc aacctgcgcg
     9901 tgatgtgtca cgatgcggtt gaagtgctgc ataaaatgat tcctgacaat tcattgcgca
     9961 tggtgcagct ctttttccct gacccgtggc acaaagcgcg ccataataaa cgccgtatcg
    10021 ttcaggtgcc gtttgccgaa ctggtaaaaa gcaaactgca gctggggggc gtattccata
    10081 tggcgaccga ctgggaacct tatgcggaac atatgattga agtgatgtct tctattgacg
    10141 gttataaaaa cctgtcagag agcaatgatt acgtaccgcg tccggcatca cgtccggtga
    10201 cgaaatttga acaacgtggt catcgtcttg gtcacggagt atgggactta atgttcgaga
    10261 gggtgaaata atggcaaaga accgtagccg tcgtctgcgt aaaaaaatgc acatcgacga
    10321 attccaggaa ttaggatttt cggtggcatg gcgattcccg gaaggtacat cggaagaaca
    10381 gattgataaa accgttgatg attttattaa cgaggttatc gaaccgaaca aactggcctt
    10441 tgacggcagc ggttatctgg cctgggaagg tctgatctgc atgcaggaaa tcggcaaatg
    10501 caccgaagaa catcaggcga ttgtgcgtaa gtggctggaa gagcgcaacc tggatgaggt
    10561 acgcaccagc gaacttttcg acgtttggtg ggactaagaa agcatacggg cgatgacaaa
    10621 tgcaaaactg cctgatgcgc tacgcttatc atgcctacaa agatgcacga tcgagtaggc
    10681 gggataaggc gtttacgccg catccggcat ggaaaacgcg tactttgtta tcaatctggg
    10741 gccagcaaat gctggcctga tttgttcttg agggaagact atgatgcgca aaatgctgct
    10801 ggcggcagca ctttcagtga cggcaatgac cgctcacgcc gactaccagt gcagcgtcac
    10861 gccgcgtgac gatgtgattg tcagcccgca aaccgtgcag gtgaagggcg aaaacggcaa
    10921 tctggtgatc acgccagacg gcaacgtgat gtataacggt aagcaatatt ccctgaatgc
    10981 cgcccagcgc gagcaggcga aggattatca ggctgaacta cgtagcacgc tgccgtggat
    11041 tgatgaaggc gcgaaaagcc gcgtcgagaa agcccgtatt gctctggata aaattatcgt
    11101 tcaggagatg ggcgaaagca gcaaaatgcg cagccgtctg accaaacttg atgcgcagct
    11161 gaaagagcag atgaaccgca ttatcgaaac gcgcagcgat ggcctgacgt tccactataa
    11221 agccattgat caggttcgcg ctgaaggcca gcaattagtg aatcaggcaa tgggcggaat
    11281 tttacaggac agcattaatg aaatgggcgc gaaagcggtg ctgaaaagcg gcggtaaccc
    11341 attacagaat gtgctgggaa gcctgggggg gctgcaatcc tcaatccaaa ccgagtggaa
    11401 aaagcaggaa aaagatttcc agcagtttgg caaagatgtt tgtagccgcg ttgtgactct
    11461 ggaagatagc cgcaaagccc tggtcgggaa tttaaaataa tcctctattt taagacggca
    11521 taatactttt ttatgccgtt taattcttcg ttttgttacc tgcctctaac tttgtagatc
    11581 tccaaaatat attcacgttg taaattgttt aacgtcaaat ttcccataca gagctaaggg
    11641 ataatgcgta gcgttcacgt aactggagga atgaaatgga gtttttcaaa aagacggcac
    11701 ttgccgcact ggttatgggt tttagtggtg cagcattggc attacccaat atcaccattt
    11761 tagcaaccgg cgggaccatt gccggtggtg gtgactccgc aaccaaatct aactacacag
    11821 cgggtaaagt tggcgtagaa aatctggtta atgcggtgcc gcaactgaag gacattgcga
    11881 acgttaaagg cgagcaggta gtgaatatcg gctcccagga catgaacgat gatgtctggc
    11941 tgacactggc gaaaaaaatt aacaccgact gcgataaaac cgacggcttc gtcattactc
    12001 acggtaccga cacgatggaa gaaaccgctt acttcctcga cctgacggtg aaatgcgaca
    12061 aaccggtggt gatggtcggc gcaatgcgtc cgtccacgtc tatgagcgca gacggtccat
    12121 tcaacctgta taacgcggta gtgaccgcag ctgataaagc ctccgctaat cgtggcgtgc
    12181 tggtggtgat gaacgacacc gtactggacg gtcgcgatgt caccaaaacc aacaccaccg
    12241 acgtagcgac cttcaagtct gttaactacg gtccgctggg atacattcac aacggtaaga
    12301 ttgactacca acgtaccccg gcacgtaagc acaccagcga cacgccgttc gatgtctcta
    12361 agctgaatga actgccgaaa gtcggcattg tttataacta cgctaacgca tccgatcttc
    12421 cggctaaagc cctggtagat gcgggctatg atggcatcgt gagcgctggt gtgggtaacg
    12481 gcaacctgta taaatccgtg tttgacacgc tggcgaccgc cgcgaaaaac ggtactgcag
    12541 tcgtgcgttc ttcccgcgta ccgacgggcg ctaccactca ggatgccgaa gtggatgatg
    12601 cgaaatacgg ttttattgcc tctggtacgc tgaacccgca aaaagcgcgc gtcctgctgc
    12661 aactggctct gacgcaaacc aaagatccgc agcagatcca gcagatcttc aatcagtact
    12721 aatcatttcg ccccggtatc gcgccggggc tttttcactt cagacttacg tccattgcca
    12781 attttaattg ccctaatgat aatcaccaga ataaattatt ccgcgcgagg gttttcgggt
    12841 gaaaaagcaa tggattgttg gcacggcgct gcttatgttg atgactggta atgtctgggc
    12901 agatggcgaa ccgccaactg aaaatatctt aaaagatcaa ttcaaaaagc agtatcacgg
    12961 cattctcaag cttgatgcca tcaccttaaa aaatcttgat gctaagggta atcaggccac
    13021 ctggtcagcg gaaggcgatg tctcttccag tgacgatctc tatacctggg tcggtcagtt
    13081 ggcagattac gaactgctcg aacagacctg gacgaaagat aaaccggtaa aattctcggc
    13141 gatgttaacc agtaaaggaa cgcccgcgtc tggctggacg gtgaactttt actcttttca
    13201 ggcgacagct agcgatcgtg gtcgggtggt cgacgatatc aaaacgaata ataaatatct
    13261 gatcgtgaat agcgaagatt tcaattatcg ctttagtcag cttgagactg cgttgaatac
    13321 ccagaagaac tcgattcctg cgctggaaaa ggaagtgaaa gcgcttgata agcaaatggt
    13381 ggcggcgcag aaagcagcgg atgcatattg gggaaaagat gcaaatggta agcaaatgac
    13441 ccgcgaagat gcctttaaaa aaattcacca acagcgtgat gagtttaata aacagaacga
    13501 tagcgaggcg ttcgcggtta aatatgacaa agaggtttat caaccggcga ttgcggcatg
    13561 tcataaacag agtgaagagt gttatgaagt gccgattcag cagaaacgag atttcgatat
    13621 caacgaacaa cggcgacaga cttttctgca atcacaaaaa cttagccgta aattgcagga
    13681 tgactggata acgctcgaaa aagggcaata tccgttaacg atgaaagttt cagaaataaa
    13741 cagtaaaaaa gtcgcgattt tgatgaaaat tgacgatatc aaccaggcta atgaacgctg
    13801 gaaaaaagat accgaacaac tgcggcgcaa tggggtgatt aagtaagttt tgatatagcg
    13861 cacgatttgt agcaggatgc ggcgttgacc ccgcatccgg caatcaacgt ctgatgcgac
    13921 gctagcgcgt cttatcagac ctacaacagc tgccacatgt cggctgaggt gctcacgccg
    13981 catccggcaa tacaagttta ctcagccaga aaaagctcca gcagcgaatt taaaaacagc
    14041 ttcccatgtt ccgtaatctg ccagtaatcc gcacattcgg tgagataacc ctgggcaatc
    14101 gcctcgtcta actgtgggcg aatcacatct tcgcaaagcc cggtatacgc actaaactcc
    14161 acgcgcggcg caggttccag caaccggaag cgattcataa agaactcaaa cggcttatct
    14221 gcggcttcga catcacgctg gctttccaga taccttcctt gcataaaacc acgcggatga
    14281 cgcgttttgg tggtacgcag aatgcgccca tccgggaagg tcactttgcc gtgcgcgccg
    14341 cagccaatac cgatgtagtc accaaagcgc cagtagttga gattgtgctg gcactgataa
    14401 ccgggtttgg cgtaagcgga agtttcatat tgctgataac ccgctgcggt taataactga
    14461 tgcccctgtt cgaaaatatc ccacagcgcg tcgtcgtccg gcagtaccgg cgggcgtgaa
    14521 ccaaacagcg tattaggttc gatggtcagt tgataccagg aaagatgcgg cggattcagt
    14581 tcaatagcct ggcgcagatc gccaagcgcc tcttccagcg attgatccgg tagcccatgc
    14641 atcaaatcaa ggttaaagct acgtaaccct aaaccgctcg ccagctttgc cgctcgtttc
    14701 gcttcttgcg ggccatgaat acgtcccagc cgtttcagct tttcttcgct aaaactctgt
    14761 accccaatag agatacgatt tacaccggca cgctgataat caacaaagcg atcggcttct
    14821 accgtaccgg ggttcgcttc catggtaatt tctgcatccg ctgccagcgg caaacgcgca
    14881 cgcacgccgt ccagcagcgt ttgcatcgcc gagccggaaa gcaggctcgg cgtaccaccg
    14941 ccaataaaga ttgtctttac ttcacggccc tgagcgtaag ccacatcgtt gtccagatcg
    15001 ttaagcagat gctgaacgta atcgtcgtgc ggcacttctc ctttcaacgc gtgagagttg
    15061 aaatcgcagt acgggcattt ctgcacgcac cacgggatgt gaatgtagag actcagcggc
    15121 ggtaatttaa ccattacgta aagcgtccag cagcagtttc aatgcctgac cacggtggga
    15181 aatggcgctc ttttcttcgc gggtcagttc ggcagcggtt ttcccttcgg aaggtacgaa
    15241 gaagattgga tcataaccaa agccaccagt gcccgccggt tcgcgagtaa tcacacccgg
    15301 ccagctgcca tggcacacca gcggggtcgg atcttctgcg tgacgcagat acaccagcac
    15361 gcagtggaaa cgcgcctgac gttggtcgtc cggtacgtct ttcagtgttt ccagcagttt
    15421 ttgcagattc ttttgatcgg tcgcgtcttc accggaataa cgtgcggagt aaatacccgg
    15481 cgcgccgcca agcacatcta ccgccaggcc agagtcgtcg gcaattgccg gtaaaccggt
    15541 cacttttgcc gcgtggcgcg ctttcagaat cgcgttttcg ataaaggtca ggccggtttc
    15601 ttcagcggaa tcaacgccga gttctgtttg ggccacgata tcaagaccga agtcgctaag
    15661 cagcgacgcc agctcacgca ctttaccggc attgccggtt gcgaggacaa ctttttgcat
    15721 aggataccta attaattaac gccgcgactt ctggcgggat ttgttgcgga ttaatgattt
    15781 taatttgttt gtggcggcca agttcgcctt tttcaatcac cacctggctt ttggcaaccc
    15841 ggaattgctt accgagaaac ttcaccagat ggctgttggc ctgaccgtca accggcggcg
    15901 cggtaatggc gacttttacc tcgtcgccat gtaaaccgac aatagaatca cggctggctt
    15961 tcggctgaat atagagccgt aaaaccagac cgtcatcgtt aactgttacg gcactcataa
    16021 cgccatccac agccccggca gcagcatatt tccggtcgcc tgtaatactt ctgcgacacc
    16081 catattgatg acatacagca gcagaacgag gatcatcggc gagaaatcaa tcccccccat
    16141 tgccggtagc aagcggcgaa tcgggcgcag cagcggatcg gccagctgaa tcagcacgta
    16201 ttcaatcggg ctacggccct ggcttaccca gctcataatc gccatcacca gcaggaccca
    16261 gaaaatcagc aggccgatgg ttttcagcag aatcagtaaa ccggcaatcc agatgattgg
    16321 caggaaggtc accactttaa acagcacgat ggctttgata aaactgagaa tataggcaac
    16381 cagcagcgag gcgctatcaa tgggtcccat tgccggaata acgcggcgca gtggcccgat
    16441 aattggctgc gttaccttca ctacaaactg tgagaagggg ttgtaaaaat cacaatgagc
    16501 ccactgcatc cagatgcgta ataacagcac catggtatac agctcaatga ccgttgaaag
    16561 caggaaagtc aacgtattca tggcgttcct ttaattcctt attttttaga gtaatcacgc
    16621 gcaccaaaaa ttgcagtgcc gatacgaacc atcgtgctac ctgccgcaat ggcggcttcc
    16681 atatcgtccg acattcccag agagagcgtg tcgatatgcg ggtagcgcgt tttcagtccg
    16741 gcaaatgcta cagccatttg gcgtgcaact tcaaactgcc ttacatattc tgactcaggc
    16801 gcagggattg ccatcagccc gcgcagacgt aaacgcggta gttcagcgac cgcagctgcc
    16861 agctcgtcca gttctgccag ttgaatcccg gacttactgt tttcatcact aatattaatt
    16921 tgaatcagta cgttaagagg gggaagttct gccgggcgct gatcgttgag gcgggtagca
    16981 atgcgcaaac ggtcgatggt atgacaccag tcgaaatgct ctgccaccag gcggctttta
    17041 ttagactgca acgggccaat aaaatgccat tctaatcctg ttacgcccag ttcctgaaag
    17101 tggcgaattt tatctacccc ttcctgaacg tagttttcac caaattgacg ctgcccggca
    17161 tcaatggctt ctgcgatggc gctcgcaggt tttgttttac tgactgcaag cagcgtaatt
    17221 tcttctggag aacggccgca acgcgttgca gccgctgaga ttttgtcccg gacctgtgcc
    17281 aggttatgcg caatatcgtt cattttccga ggatcttagt atgaatatgg aagaaattgt
    17341 ggcccttagt gtaaagcata acgtctcgga tctacacctg tgcagcgcct ggcccgcacg
    17401 atggcgcatt cgcgggctaa tggaagctgc gccgtttgat gcgccggacg tcgaagagct
    17461 actgcgggag tggctggatg acgatcagcg ggcaatattg ctggagaatg gtcagctgga
    17521 ttttgccgtg tcgctgacgg aaaaccagcg attgcgtggc agcgcattcg cgcaacggca
    17581 tggcatttcg ctggcgttac ggctgttacc ttcgcactgc ccgcagctcg aacagcttgg
    17641 cacaccaccg atattgccgg agttactcaa gagcgagaat ggcttgattc tggtgacggg
    17701 ggcgacgggg agtggcaaat ctaccacgct ggcggcgatg gttggttatc tcaatcaaca
    17761 tgctgatgcg catattctga cgctggaaga tcctgtggaa tatctctatg ccagccagcg
    17821 atgtttgatc cagcagcggg aaatcggttt gcattgtatg acgttcgcat cgggattgcg
    17881 ggccgcattg cgggaagatc ctgatgtgat tttgctcgga gagctgcgtg acagcgagac
    17941 aatccgtctg gcgctgacgg cggcagaaac cgggcatctg gtgctggcaa ccttacatac
    18001 acgtggtgcc gcgcaggcag ttgagcgact tgtggattca tttccggcgc aggaaaaaga
    18061 ccccgtgcgt aatcaactgg caggtagttt acgggcagtg ctgtcacaaa agctggaagt
    18121 ggataaacag gaaggacgcg tggcgctatt tgaattgctg attaacacac ccgcggtggg
    18181 gaatttgatt cgcgaaggga aaacccacca gttaccgcat gttattcaaa ctgggcaaca
    18241 ggtggggatg ataacgtttc agcagagtta tcagcagcgg gtgggggaag ggcgtttgtg
    18301 acgttgccat gcccgatgcg caagcttatc gggcctacaa aggcaataaa cgtctgatgc
    18361 gacgcttgcg cgtcttatca gacctacaac cgctgccaca tgtaggccgg ataaggcgtt
    18421 cacgccgcat ccggcaataa ggcgtaaggc gcgatgatat tatttacact ccagcaggcg
    18481 attaaatctc cttaatatcc ctgctcgaaa tagctttcga gaataataac cgcagaggca
    18541 gagtcgattt tgcctttgtt gagcgcccga tagccgccct gttcaaacag accggaacgt
    18601 gcctccacgg tgctaagacg ctcgtcatgg agctttactt caacaccgaa acgaccatga
    18661 atacggttag caaatttacg cgctctggcg gttaacggct gttcggtgcc gtccatattc
    18721 agcggtaaac cgacgatgat ttcgtctggc tgccactctt tcagtaaacg ttcgatgagg
    18781 ttccagtccg gcgtaccgtc ctgtgcttta attgcaggca aagggcgagc ggtgccggta
    18841 atgcgttggc cgaccgctac gccaatgctt ttggtgccga agtcgaaggc gagtaaggtt
    18901 ccactcatca ggcgtgtcct gccacaccag gcatggtgag aatatccaca ccaatcagtt
    18961 ttgccgcctc gcgccagcgg tcggcaattg gcgttttgaa cagaatattc agatctgccg
    19021 gggcggttag ccacgcgtta tcgagaattt cttgttccag ttgacctttt tcccaggagg
    19081 catagcccag agccaccaat acgtcagacg gttgtttatc ggtgccgagc gtttccagca
    19141 catcgcggga agtggtcatc actgtgttgt ctgaaatgcg aatgctggaa gcaaaattgg
    19201 agggcggagt atgcaaaata aacccgcgat cttcagccag tgggccgccg agcataaccg
    19261 gtttatccag acggattgat tcatcacgcg gctccggcgt gatcttcagc ttttccagaa
    19321 tcccttcaat tttgagattt tccagcggct tgttgacgat gatccccatt gcaccattgg
    19381 tattatgttc gcaaatgtag accacggaac gacggaaaat cggatcctgg agagcaggca
    19441 tggcaataag aaagtgatgc tgtaaattca ttgtcagagg ttctgtttcc tggttcaaaa
    19501 aagcaacagc gcccagtatg cgtggaaaga agggcgctgt cactcagagt ctcaacgaga
    19561 tccttctcgc taaggtgggt tactactgct gtaaacgtgc ttcgatggca tccattaaca
    19621 ttccggtgat cgacaccgga aactctgctt caatctcacg aatacaggtt gggctggtga
    19681 cgttaatttc agtcagacgg tcgccgatga tatccagacc aacaaaaatc agcccttttt
    19741 ctttcagcgt cggcccgatc tgacgggcga ttttccagtc actttccgtc agcggacgag
    19801 gttcaccgcg accaccggca gccagattgc cacgggtttc gcccccctgc ggaatacgcg
    19861 ccaggcagta cggtactggt tcgccgtcca ccaccagcac gcgtttgtcg ccatctttaa
    19921 tggctggcag ataattttgt gccatgcagt agcgagtgcc gtgttcggtc aaggtttcgg
    19981 caatgacgcc gaggtttgga tcgccttctt tcacgcggaa aatcgacgcg ccgcccatac
    20041 cgtccagcgg cttaagaatg atgtcgctgt gtttctccca gaacgctttt agctgcgctt
    20101 tattgcgcgt aaccagcgtt tctggcgtta agtcagagaa ccaggcggta aacagtttct
    20161 cgttacagtc gcgcaggctc tgcggcttgt taacgatcag cgtccctttc tcttcggcac
    20221 gttccagaat ataggtcgcg tagataaact cggtatcaaa cggcgggtct ttacgcatca
    20281 ggatcacatc gagatcggcc agcggcagat cctgttcacc gacgaatgaa aaccactctt
    20341 cgtagttctg tttcacgttc agcgtgcggg tatgggcgcg ggcttcgcca ttgatcagat
    20401 acagatcgcc catctccata tagtgaagtt cgtaaccacg acgctgtgct tccagcaaca
    20461 tagcgaaact ggaatctttc ttgatgttga tgtttgcgat ggggtccatc acgatgccga
    20521 gcttgatcat tattcttctc cgttagccca aatcgccaaa tcgtacttgt agcgcggtaa
    20581 tggcggtgag cgcagttgtc tctgtacgca aaacgcgagg tcccaacagg atatcagtaa
    20641 attgatagcg ggcagtcatg gcaatttcat ctgccgataa accgccttcc gggccaatca
    20701 gcaggcggac gcgttcaacc ggtaaaggca acgtattgat gctgttactg gcgcgcgggt
    20761 gaagattcag tttcagtcct tcatcctgct ctgcacacca ggcttccaga tccatcgctg
    20821 gacggatttc cggcacccgg ttacgaccac actgctcaca ggcagcaatt gcaatcttct
    20881 gccactgctg gagcttcttg ttcagacgtt cactatccag tttaacgccg cagcgctcag
    20941 aaaaaagtgg cgtaatgagg cttacaccga gttcgatcga tttctggatc gtaaattcca
    21001 ttttttcacc acgcgacatc acctgaccga ggtgaatatg cagcggagat tcgcgatcgt
    21061 cgatctggcc ttccagcacc ttcacttcca cgcttttttt gctggcgctg gtaatttcgg
    21121 cgtcaaagac ctggttgctg ccgtcaaaca attgcaacgc ctgtcccggc cccatacgca
    21181 gtacgcgccc aatatggttg gcggcatctt cgcaaagcgc gatgtgagaa tggctggtca
    21241 gtggttcagg atgataaatg cgggggatac gcatagttaa aaatccgcgt cgtctcccca
    21301 cgcggttgta cgcgtggggt aggggttaac aaaaagaatc ccgctagtgt aggttagctc
    21361 tttcgcgcct ggcaagcgcg ttgcacatac gggttatgat tgccctgtac cttcgcgatg
    21421 cgttcatcgc gctcgcactc ccagtcggta accggataca tcttgtccca tgcgttgaac
    21481 agctgcgttt gctggcgaga gagtgtcagg ttgtaatggt cacgcatata gaagtaggtg
    21541 cgcgcaatgg caccgcgtgc acgcgctggt ggttcggcag ctttttcttt gaaatcgacc
    21601 ttcatggcgc attgaccgta ctggccttca ccgccattcc actggctgta cataaagttg
    21661 ccgcgatcgc cattcacctc gccgactgac ggttgcaggt tatgcatatc gctttccatc
    21721 ttgcgataga ccggatcttt ggcgcaattt ttacgtccac cgtcctgcca gcactggcgc
    21781 tggtgaccga attgccaggc gggaacgacg tgttcccact ctacgcggct ggcgcggttt
    21841 tcatttttgc gcacctgata gccgcacgat tgcagatcga caacgccttt tttgccctgc
    21901 cagttaattt tacatccgca ataaaacgta ccgggcgcgt cagcgtggac ttttaccgcc
    21961 gcggctttcg cctgagaaaa actattgata ccttcggcca acgccgggcc ggaaaatgct
    22021 gcgctcagta ccaccgcagc aatagacaaa taacggtaca tcacttactc cgtgttaaaa
    22081 cgagccagca acgtagcgaa agttgttttg gtatgcaatc agccagatca ggaaagttcc
    22141 tgatagttca gatggttatt ttgcgaccag ttgttcgccg cagtgaacac agcgatagac
    22201 ggcttcgcca cgaactacgc gattatgacg gcgtacggta agctgatgtt cctgacactt
    22261 acagctgtag gggaaggtgt tgcggcgcac ggattgcagt tcgaactgat gcgtacggcg
    22321 ggctggaacg cccagcacac tttccatcat ccacttccac tctttgccat gtggtgctac
    22381 gcggccaaaa tgtttccata ccagcaaatg tgccagttcg tgcggtacca cttcttcaat
    22441 aaaggcttca ctgttttcca acagcaaaac gggattaagg cgaatttcat agctttccag
    22501 ccaggctgtt ccggcagagg ttccgcgctg ggtgtaagag agttttggct ccgggtagtt
    22561 acgccccaat ttcaggttgg cctgggcgag tttttcccgc aggcgacgca taacggcctg
    22621 ctggatggcg atagggagac gggatgtttt catagcggca gaggatagag cgaagaaaag
    22681 atgactgcaa gaggtggctt cctccacgac gggaggaagc ctggggagat taatcgtgag
    22741 cgccgatttc gcgcagttta cgacctttca tcagattacg ttcaatatgt tccagcgaaa
    22801 cgtgtttggt ttctggcacc agccacagtg tcagcaggat aaacaggacg ttcagacccg
    22861 cgtaaaccca gaaggtatta gcgttgccca gcgtgttgag catggtcagg aacgttgcgc
    22921 caacgatcat gttggcaatc cagttggtgg cggtggagca ggtgatgcca aaatcgcggc
    22981 ctttcagcgg ctgaatttcg gagcacagta cccaaatcag cggaccggca ctcatggcaa
    23041 aaccgacaat aaacatcagc agcatggcga tggcgaaata ctgcgccgac ggagagtgga
    23101 taccgatatg catcattgta ccgagtacgc ccataccagc agccatcacc aggaagccca
    23161 gcgttagcgt tggtttacgt ccccagcggt caacaaggcc gattgcgata aaggtggcaa
    23221 gtacgttggt caggccgaca atcactgtcc cccacatttg ctcggtggtg ttggtataac
    23281 ccgccagttc gaagattttc ggcgcgtaat acatgatgac gttcatcccg gtgaattgct
    23341 gcattacctg taacagtacg ccaaggaaca ccgcgcggcg gaagttgcta ttctctttaa
    23401 acagcgccca gccactctgt ttaacctgca aactttcacg gatttcatcc agttcgcgtt
    23461 tcgcttccgc gctggtgtca cgcaggcgta gcagcacgcg ttcggcatca acaaaacggc
    23521 gtttggcggc aaaccaacgt gggctgtctg gcaggaagaa gacaccaatc agcagcaaaa
    23581 ttgccgggat gataatcaca cccagcatcc agcgccatgc accggtgtag ctgaaggcgg
    23641 tatcagaaag ataagcaccg aggatcccga ttgtgatcat taactgatac atcgagatca
    23701 tactgccgcg aattttttcc ggcgcaattt cagagaggta cagtggtgcg gtataagagg
    23761 ccacacctac cgccagcccc agcagaacgc gggaaagaat cagtacttca acgtttggcg
    23821 cagccgcaga gaacagcgaa ccggcaacaa acaaaattgc gccgatcatc aggctctttt
    23881 tgcgcccgag tttaaaggag agccagccgc tgcccaccgc accgactgcc gcaccgaaca
    23941 tcatggagct tacgacccat tcttgcgtgt gcgaagtaat ctggaattca tctgcaataa
    24001 acggcagtgc gccagcaatt acaccgatat ccaggccaaa gagtaatccc gccagagcgg
    24061 caaggaagca gacgaaaaac gtcattgcct tgtttgactg cccctgtttt ttagcgtcag
    24121 gcatgatgcc ctccaatatg gttatttttt attgtgaatt aagataggtg agtacgacgt
    24181 aaaaagatgt gaagcaaatc acatcagtgt aatcgctt
//
LOCUS       DAZKIF010000071        24062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZKIF010000071 DAZKIF010000000
VERSION     DAZKIF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.071"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            168..503
                     /gene="yjdM"
                     /locus_tag="ACHOTW_002772"
     CDS             168..503
                     /gene="yjdM"
                     /locus_tag="ACHOTW_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9844848.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            762..1205
                     /gene="yjdN"
                     /locus_tag="ACHOTW_002773"
     CDS             762..1205
                     /gene="yjdN"
                     /locus_tag="ACHOTW_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP9844849.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1338..2126
                     /gene="phnC"
                     /locus_tag="ACHOTW_002774"
     CDS             1338..2126
                     /gene="phnC"
                     /locus_tag="ACHOTW_002774"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP9844850.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2151..3167
                     /gene="phnD"
                     /locus_tag="ACHOTW_002775"
     CDS             2151..3167
                     /gene="phnD"
                     /locus_tag="ACHOTW_002775"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9844851.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            3273..4052
                     /gene="phnE"
                     /locus_tag="ACHOTW_002776"
     CDS             3273..4052
                     /gene="phnE"
                     /locus_tag="ACHOTW_002776"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9844852.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4073..4798
                     /gene="phnF"
                     /locus_tag="ACHOTW_002777"
     CDS             4073..4798
                     /gene="phnF"
                     /locus_tag="ACHOTW_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP9844853.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4799..5251
                     /gene="phnG"
                     /locus_tag="ACHOTW_002778"
     CDS             4799..5251
                     /gene="phnG"
                     /locus_tag="ACHOTW_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP9844854.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5248..5832
                     /gene="phnH"
                     /locus_tag="ACHOTW_002779"
     CDS             5248..5832
                     /gene="phnH"
                     /locus_tag="ACHOTW_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP9844855.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5832..6896
                     /gene="phnI"
                     /locus_tag="ACHOTW_002780"
     CDS             5832..6896
                     /gene="phnI"
                     /locus_tag="ACHOTW_002780"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP9844856.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6889..7734
                     /gene="phnJ"
                     /locus_tag="ACHOTW_002781"
     CDS             6889..7734
                     /gene="phnJ"
                     /locus_tag="ACHOTW_002781"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP9844857.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7731..8489
                     /gene="phnK"
                     /locus_tag="ACHOTW_002782"
     CDS             7731..8489
                     /gene="phnK"
                     /locus_tag="ACHOTW_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP9844858.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8600..9280
                     /gene="phnL"
                     /locus_tag="ACHOTW_002783"
     CDS             8600..9280
                     /gene="phnL"
                     /locus_tag="ACHOTW_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP9844859.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9277..10413
                     /gene="phnM"
                     /locus_tag="ACHOTW_002784"
     CDS             9277..10413
                     /gene="phnM"
                     /locus_tag="ACHOTW_002784"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP9844860.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10413..10970
                     /gene="phnN"
                     /locus_tag="ACHOTW_002785"
     CDS             10413..10970
                     /gene="phnN"
                     /locus_tag="ACHOTW_002785"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP9844861.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10957..11392
                     /gene="phnO"
                     /locus_tag="ACHOTW_002786"
                     /pseudo
     CDS             10957..11392
                     /gene="phnO"
                     /locus_tag="ACHOTW_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            11394..12152
                     /gene="phnP"
                     /locus_tag="ACHOTW_002787"
     CDS             11394..12152
                     /gene="phnP"
                     /locus_tag="ACHOTW_002787"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP9844862.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12229..12558)
                     /gene="yjdP"
                     /locus_tag="ACHOTW_002788"
     CDS             complement(12229..12558)
                     /gene="yjdP"
                     /locus_tag="ACHOTW_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP9844863.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(12627..14897)
                     /locus_tag="ACHOTW_002789"
     CDS             complement(12627..14897)
                     /locus_tag="ACHOTW_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9844864.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            15011..16531
                     /locus_tag="ACHOTW_002790"
     CDS             15011..16531
                     /locus_tag="ACHOTW_002790"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9844865.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16528..17502
                     /locus_tag="ACHOTW_002791"
     CDS             16528..17502
                     /locus_tag="ACHOTW_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP9844866.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            17521..18462
                     /locus_tag="ACHOTW_002792"
     CDS             17521..18462
                     /locus_tag="ACHOTW_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9844867.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18517..19200
                     /locus_tag="ACHOTW_002793"
     CDS             18517..19200
                     /locus_tag="ACHOTW_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFP9844868.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19215..20075
                     /locus_tag="ACHOTW_002794"
     CDS             19215..20075
                     /locus_tag="ACHOTW_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFP9844869.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            20072..21073
                     /locus_tag="ACHOTW_002795"
     CDS             20072..21073
                     /locus_tag="ACHOTW_002795"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP9844870.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21070..21777)
                     /locus_tag="ACHOTW_002796"
     CDS             complement(21070..21777)
                     /locus_tag="ACHOTW_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9844871.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            22068..24053
                     /gene="yjcS"
                     /locus_tag="ACHOTW_002797"
     CDS             22068..24053
                     /gene="yjcS"
                     /locus_tag="ACHOTW_002797"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFP9844872.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5356 a         7014 c         6738 g         4954 t
ORIGIN      
        1 gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga aaacgatcgt cgtctaaaat
       61 cagcagtacc cccgacaaac tcagggattt tgtgtataat tgcggccttt ttcggcaatc
      121 tgccgttttt tggcgctttt gccctgctga cttttgagga aatccacatg tcattaccac
      181 actgcccaaa atgcaactcc gaatacactt acgaagataa cggcatgtac atctgcccgg
      241 aatgtgccta cgaatggaac gacgcagaac ctgcacagga aagcgacgag ctgatcgtta
      301 aagatgctaa cggcaatctg ctggctgacg gcgacagcgt taccatcatt aaagatctga
      361 aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa agtgaaaaac atccgcctgg
      421 ttgaaggcga ccataacatc gattgcaaaa tcgacggttt tggtccgatg aaactgaaat
      481 ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta agccggataa ggcgtttacg
      541 ccgcatccgg caacagtgcc agatgcctga tgcgacgctc gcgcgtctta tcaggcctac
      601 aaattcccgc accctccgta ggccggataa ggcgtttacg ccgcatccgg caacagtgcc
      661 gactgcctga tgcgacgctc gcgcgtctta tcaggcttcc ctctcatctg tataaatttc
      721 gaactacact taactggctt ctcttaactg aggtcaccat catgccgtta agtccctacc
      781 tctcttttgc cggtaactgc tccgacgcga ttgcctatta tcaacgtacg ttgggcgctg
      841 aactgctcta taaaatcagc ttcggcgaaa tgccaaaatc agcgcaggac agcgccgaga
      901 actgcccttc cggaatgcaa tttcccgata ccgccatcgc ccacgccaat gtgcgcattg
      961 ccggaagcga tatcatgatg agcgatgcta taccgtcagg aaaagccagc tactccggtt
     1021 ttacgctggt gctcgattcg caacaggtcg aagaaggaaa acgctggttt gataaccttg
     1081 ccgctaacgg aaaaatcgaa atggcctggc aggaaacttt ctgggcgcat ggctttggca
     1141 aagtcaccga taaatttggc gtaccgtgga tgattaatgt cgtcaaacaa caaccaacgc
     1201 aataatccgc cgggaggccc gccctcccgc actgtcatcg aattcccgtt aactcttcat
     1261 ctgttagtca cttttaatta accaaatcgt cacaataatc cgccacgatg gagccacttt
     1321 tttagggagg ctgcatcatg caaacgatta tctgcgtcga acaactgagc aaaaccttca
     1381 accagcatca ggcgctgcac gcggttgatc tgaatatcca tcatggtgaa atggtggctc
     1441 tgcttgggcc gtccggttcc ggcaaatcca cccttttacg tcacttaagc ggtttgatta
     1501 ccggcgataa atccgccggt agccatatcg agctgctggg ccgcactgtc cagcgcgaag
     1561 gccgcctggc ccgcgatatc cgcaaaagcc gcgccaacac cggctacatc ttccaacaat
     1621 tcaacctggt gaatcgcctg agcgtactgg agaacgtgct gattggcgcg ctcggcagca
     1681 cgccgttctg gcgcacctgt tttagttggt tcacccgcga gcagaaacag cgcgcattac
     1741 aggcgctgac ccgcgtcggt atggcgcact ttgcgtatca gcgtgtttcc acgctctccg
     1801 gcggccagca gcagcgtgtg gcgattgccc gcgcgctgat gcagcaggcg aaagtgattc
     1861 tcgctgacga accgatcgcc tcgctggacc cggaatcggc gcgcatcgtg atggacaccc
     1921 tgcgcgacat caaccagaac gacggcatca ccgtggtcgt cacgctgcat caggtggatt
     1981 acgccctgcg ctactgcgaa cgcatcgtcg ccctgcgcca ggggcacgtc ttctacgacg
     2041 gcagcagcca acagtttgat aacgaacgtt ttgaccatct ctaccgcagc atcaaccgcg
     2101 tcgaagagaa cgcgaaagct gcctgacatc cccatcattg aggaaaacga atgaacgcta
     2161 agataattgc ctcgctggcc ttcaccagca tgttcagcct cagcaccctg ttaagcccgg
     2221 cacacgccga agagcaggaa aaggcgctga atttcggcat tatttcaacg gaatcacagc
     2281 aaaacctgaa accgcaatgg acgccgtttt tacaggatat ggagaagaag ctgggcgtga
     2341 aggtcaacgc cttctttgcc ccggactatg cgggcattat ccagggaatg cgtttcaaca
     2401 aagtggatat cgcctggtac ggcaacctgt cggcgatgga agcagtggat cgcgccaacg
     2461 gccaagtctt cgctcagacc gtcgcggcgg atggatcgcc gggttactgg agcgtgttga
     2521 tcgtcaacaa agacagcccg atcaacaacc tgaacgatct gctggcgaag cggaaagatc
     2581 tcaccttcgg taatggcgat cctaactcca cctctggctt cctcgtcccc ggctactacg
     2641 tcttctccaa aaacaatatc tccgccagtg acttcaagcg caccgtcaac gccgggcatg
     2701 aaaccaacgc gctggccgtc gccaacaagc aggtggatgt ggcgaccaac aacaccgaaa
     2761 acctcgacaa gctgaaaacc tccgcgccgg agaagctgaa agaactgaaa gtgatctgga
     2821 agtcaccgct gatcccaggc gatccgatcg tctggcgtaa aaacctttcc gaaaccacca
     2881 aagacaagat ctacgacttc tttatgaatt acggcaagac gccggaagag aaagcggtgc
     2941 tggaacgcct gggctgggcg ccgttccgcg cctccagcga cctgcaactg gtgccgattc
     3001 gccagctcgc actgtttaaa gagatgcaaa gcgtgaaagg caataaaggg ctgaatgagc
     3061 aggacaagct ggcaaaaacc accgagattc aagcgcagct ggatgacctg gaccgcctga
     3121 acaacgcgtt aagcgcgatg agttcggtaa gtaaagcgat gcagtaaatc gtaggtcgga
     3181 taagacgccc cggtgtcgca tccgacaatg tgcaggcgtt gatgccggat gcggtgcaag
     3241 caccttatcc ggcctacaga ccggagccaa acatgcaaac catcaccatc gccccaccca
     3301 agcgcagctg gttctcgctt ctgagctggg ccgttgtact cgccgtgttg gtcgtctcgt
     3361 ggcagggcgc ggaaatggcc ccgctcacgc tgatcaaaga cggcggcaac atggcgacct
     3421 tcgccgccga cttcttcccg cccgatttca gccagtggca ggattacctc accgaaatgc
     3481 tcgtcacgct gcaaatcgcc gtctggggca ccgcgctggc ggtggttctc tccatcccct
     3541 tcggcctgat gagcgccgaa aacctggtgc cgtggtgggt ttaccagccc gtgcgccgcc
     3601 tgatggacgc ctgccgcgcc attaacgaaa tggtcttcgc catgctgttc gtggtcgccg
     3661 tcggcctcgg cccattcgct ggtgtgctgg cgctgtttat ccacaccacc ggtgtgctct
     3721 ccaaactgct ttccgaagcg gtggaagcga ttgaacccgg cccggtggaa ggcattcgcg
     3781 ccaccggcgc caacaagctc gaagagatcc tctacggcgt gctgccgcag gtgatgccgc
     3841 tgctgatctc ctactccctc tatcgcttcg aatccaacgt ccgctcggcg accgtcgtcg
     3901 gcatggtcgg cgcgggcggg atcggcgtca cgctgtggga agcgattcgc ggtttccagt
     3961 tccagcaaac ctgcgccctg atggtgctga ttatcgtcac ggtcagcctg ctggatttcc
     4021 tctctcaacg gttgcgtaag cactttatct gattagcgag gcattgatag ctatgcactt
     4081 gtctacacat ccgaccagct acccaacacg ctatcaggag atagccgcaa aacttgagca
     4141 ggagcttcgt caacactacc gctgcggcga ctatcttccc gccgagcagc agctggcggc
     4201 gcgctttgag gtaaatcgcc acaccctgcg ccgcgccatc gaccaactgg tggaaaaagg
     4261 ctgggtacag cgccgtcagg gcgtcggcgt gctggtgctg atgcgcccgt tcgattaccc
     4321 gctcaacgcc caggcgcgtt ttagccagaa tctgctggat cagggcagcc atcccaccag
     4381 cgaaaaactg ctgtctgtgc tgcgcccggc ctccggtcac gtcgctgacg cgctggggat
     4441 aaccgagggg gagaacgtca tccatctgcg caccctgcgc cgggtcaacg gcatcgcgct
     4501 ctgtttaatc gaccactact tcgcggacct caccctctgg ccgacgctgc aacgcttcga
     4561 cagcggctcg ctgcacgatt ttctgcgcga gcaaacgggg attgcactgc gccgcagcca
     4621 gacgcggatc agcgcccgcc gcgcccaggc caaagagtgc cagcgtcttg aaattccgaa
     4681 tatgtccccg ctgctgtgcg tgcgcaccct taaccaccgt gacggtgaaa gcagcccggc
     4741 ggagtactcc gtcagcctga cgcgcgccga catgattgaa ttcactatgg agcactgaat
     4801 gcacgcagat accgcgaccc gccagcactg gatgtccgta ctggcacaca gccaaccggc
     4861 agaactggca gcccgcctga acgcgctgaa catcaccgcc aactatgagg tgatccgcgc
     4921 cgctgaaact ggcctggtac agattcaggc gcggatgggc ggcaccggcg aacgcttttt
     4981 tgccggcgac gccacgctga cccgcgccgc cgtacgtctg actgacggca ccctcggcta
     5041 cagctgggtg ctggggcgcg acaaacagca cgccgaacgc tgcgcgctga ttgacgcgct
     5101 gatgcagcaa acccgccatt ttcaaaactt atcagaaacg cttattgccc cgctggacgc
     5161 tgaccgtatg gcacgcattg ccgcacgcca ggccgaagtg aacgccagcc gggtcgactt
     5221 ctttacgatg gttcgcggag acaacgcatg accctggaaa ccgcttttat gcttcccgtg
     5281 caggatgccc agcacagttt tcgtcgcctg ttaaaggcca tgagcgagcc gggcgtgatt
     5341 gtcgccctgc atcagctcaa acgcggctgg cagccgctga atatcgccac caccagcgtg
     5401 ctgctgacgc tggccgataa cgacacgccg gtgtggcttg ccgcgccatt gagcaacgat
     5461 atcgtcagcc agagcctgcg ttttcatacc aacgcgccgc tggtcagcca gccggaacag
     5521 gcgaccttcg cggtaacgga tgaggcgatt tccagcgaac agcttaacgc cctttccacc
     5581 ggcaccgccg ttgcgccgga agcgggcgca acgctgattt tacaggtcgc cagcctgagc
     5641 ggcgggcgca tgctgcgtct caccggcgcg ggtattgccg aagaacgaat gatcgccccg
     5701 cagctgccgg agtgcattct gcacgaactc accgagcgcc cgcacccgtt cccgctcggc
     5761 atcgacctga tcctgacctg cggcgagcgc ctgctggctg ttccgcgaac cactcatgtg
     5821 gaggtgtgct gatgtacgtt gccgtgaaag ggggcgagaa ggcgatcgac gccgcccacg
     5881 ccctgcaaga gagccgacgc cggggcgata ccgatttgcc tgaactgagc gtcgcccaga
     5941 ttgaacagca gcttaatctt gcggtggatc gcgtgatgac cgaaggcggc attgccgacc
     6001 gcgagctggc ggcgctagcg ctgaaacagg ccagcggcga taacgtcgaa gcaattttcc
     6061 tgctgcgcgc ctaccgcacc acgctggcga agctcgcggt aagcgagccg ctcgacacca
     6121 cggagatgcg tctcgaacgg cgtatttcag cggtttataa agatattccc ggcggccagc
     6181 tgcttggccc aacctacgac tacacccatc gcctgctcga tttcaccctg ctggcaaacg
     6241 gcgaagcgcc gacgctgacc accgccgaca gcgaacagca gccgtcgccg cacgttttca
     6301 gcctgctggc gcgtcagggg ctggcgaagt ttgaagagga tagcggcgca cagccggatg
     6361 acatcacccg cacgccaccg gtttacccct gctcacgttc ttcccgcctg caacagctca
     6421 tgcgcggcga cgaaggctat ttgctggcgc tggcctactc cacccaacgc ggttacgggc
     6481 gcaatcaccc gttcgcgggt gaaatccgca gtggttacat cgacgtgtcg attgtgcccg
     6541 aagagctggg atttgcggtg aacgtcggcg aactgctgat gactgagtgt gaaatggtca
     6601 acggttttat cgacccaccg gatgaatctc cgcacttcac gcgcggctac gggctggtgt
     6661 tcggcatgag cgagcgcaaa gcgatggcga tggcgctggt cgaccgcgct ctgcaagccc
     6721 cggagtacgg cgagcacgcg acaggcccgg cgcaggatga agagttcgtg ctggcacatg
     6781 ccgacaacgt cgaagccgca ggctttgtct cgcacctcaa actcccgcac tacgtcgatt
     6841 tccaggccga actggagcta ctcaaacgtc tgcaacagga gcagaaccat ggctaatctg
     6901 agcggctaca actttgccta cctcgacgag cagaccaaac gcatgatccg ccgcgccatc
     6961 ttaaaagcgg tggcgatccc cggttatcag gtgccgtttg gcgggcgcga gatgccaatg
     7021 ccgtacggct ggggaaccgg cggcattcag cttaccgcca gcgtgattgg cgaaagcgac
     7081 gtgctgaagg tgattgacca gggcgcggat gacaccacca acgccgtgtc gattcgcaac
     7141 ttctttaagc gcgtgaccgg ggtaaacacc accgagcgca cggacgatgc aacggtcatc
     7201 cagacgcgtc accgcatccc cgaaacgccg ctgaccgaag atcagatcat tattttccag
     7261 gtgccgatcc ccgaaccgct gcgctttatc gagccgcgcg aaacggaaac ccgcaccatg
     7321 cacgcgctgg aggaatacgg cgtgatgcag gtgaagctgt atgaagatat cgcccgcttc
     7381 ggtcatatcg ccaccaccta cgcctatccg gtgaaggtga acgggcgcta cgtaatggac
     7441 ccgtcgccga tcccgaaatt cgataacccg aaaatggaca tgatgcccgc cctgcaactc
     7501 ttcggcgccg ggcgtgagaa gcgcatctat gcggtgccgc cgtttacccg cgtggaaagc
     7561 ctcgatttcg acgatcaccc gttcaccgtt cagcagtggg atgagccatg cgccatctgc
     7621 ggatcgaccc acagctatct tgatgaagtg gtgctggatg acgccggaaa ccgcatgttt
     7681 gtctgctccg ataccgatta ttgccgccaa cagagcgagg caaaaagcca atgaatcaac
     7741 cgttactttc ggtcaataac ctgacccacc tttacgcgcc gggcaaaggc tttagcgatg
     7801 tctcttttga tttatggccg ggggaagtgc tgggcattgt cggggaatcc ggctccggga
     7861 agaccacgct gctgaaatca atttccgcgc gtctgacgcc gcagcagggg gagattcact
     7921 atgaaaaccg ttccctctat ggcatgagcg aggccgaccg ccgtcgcctg ctgcgcaccg
     7981 aatggggcgt agtgcatcag catccgctcg acggcctgcg ccgccaggtc tcggcaggcg
     8041 gcaatatcgg cgagcggctg atggcgaccg gggcgcgtca ttacggcgat attcgcgcca
     8101 ccgcgcagaa gtggctggaa gaggtggaaa ttcccgccaa ccggatcgac gacctgccga
     8161 ccaccttttc cggcggtatg cagcagcgtt tgcagattgc ccgcaacctg gtgacgcatc
     8221 cgaagctggt gtttatggat gaaccgaccg gcgggctgga tgtgtcggtg caggcccgcc
     8281 tgctcgacct gctgcgcggc ctggtggtgg agctgaacct cgcggtagtg attgtcaccc
     8341 acgatttagg cgtcgcccgc ctgctggcgg accgtttgct ggtgatgaag caggggcaag
     8401 tggtggagag tgggttaacc gaccgcgtgc tcgacgaccc gcatcatccg tatacacagc
     8461 tgctggtgtc atcggttttg cagaattgag ccggtgccgg atgcggcgta aacgccttat
     8521 ccggcctaca aatgcgctcc ccgtaggtcg gataagacgc atcagcgtcg catccgacac
     8581 ccgaaccacg aggcgaaaaa tgattaacgt acaaaacgtc agtaaaacct tcatcctgca
     8641 ccagcaaaac ggcgtgcgct tgcccgtcct caaccgcgcc tcgctcaccg tcaacgcggg
     8701 cgaatgcgtg gtgctccacg gccactccgg cagcggcaaa tcaaccctgc tgcgctcgct
     8761 gtacgccaac tatctgcccg atgaagggca aatccagatc aaacacggtg acgagtgggt
     8821 agacctggtc actgcgccag cgcgcaaagt ggtggaaatc cgcaaaacca ccgttggctg
     8881 ggtgagccag tttctgcgcg ttatcccgcg catttcggcg ctggaagtag taatgcagcc
     8941 gctgctcgat accggcgtac cgcgtgaagc ctgcgccgct aaagccgcgc gtctgctcac
     9001 ccgtctgaac gtgccggaac gcctgtggcg ccttgcgcca tcgaccttct ccggcggcga
     9061 acagcagcgc gtcaacatcg cccgtggctt tatcgtcgac taccccattc tgctgctcga
     9121 cgaacctacc gcctcgctgg acgccaaaaa cagcgccgcc gtagtcgaac tgatccgaga
     9181 agccaaagcc cgcggcgcag ccatcgtcgg cattttccat gacgaagccg tacgtaatga
     9241 cgtcgccgac cgcctgcacc caatgggagc ctcttcatga ttatcaataa cgtcaaactg
     9301 gtgctggaaa acgaagtggt aaacggttcg ctggagatgc aggacggcga aatccgcgcc
     9361 tttgccgaaa gccagagccg cctgccggag gcgatggacg gcgaaggcgg ctggctgctg
     9421 ccggggctga ttgagctgca tacagacaac ctcgataaat tcttcacccc gcgcccgaaa
     9481 gttgactggc ccgcccactc ggcgatgagc agccatgacg cgctgatggt ggcgagcggc
     9541 atcaccaccg tactggacgc cgtggcgatt ggcgacgtgc gagacggcgg cgatcggctg
     9601 gagaatctgg agaagatgat caacgccatc gaagagacgc agaaacgcgg cgtcaaccgc
     9661 gccgagcacc gcctgcacct gcgctgcgag ctgccgcata acaccacgct gccgctgttt
     9721 gaaaagctgg tgcagcgcga gccggtgacg ctggtgtcgc tgatggatca ctcgccgggc
     9781 cagcgccagt tcgccaaccg cgagaagtat cgcgaatatt atcagggcaa atactcgctc
     9841 accgatgcgc agatgcagca gtatgaagag gaacagctgg cgcttgccgc acgctggtcg
     9901 cagccgaatc gcgaatccat cgccgcaatg tgccgcgcgc gacaaattgc gctcgccagc
     9961 cacgatgacg caacgcacgc ccacgttgcc gaatctcacc agcttggcag cgtgatcgcc
    10021 gaatttccca ccacgtttga agcagcggaa gcctcgcgca agcatggcat gaacgtgctg
    10081 atgggcgcgc caaatatcgt gcgtggcggt tcgcactccg gcaacgtggc ggccagtgaa
    10141 ctggcacagc ttggcctgct ggatatcctc tcttccgact actaccccgc cagcctgctg
    10201 gatgcggcgt tccgcgtcgc cgatgaccag agcaaccgct ttacgctgcc gcaggcggtg
    10261 aagctggtga ccaaaaatcc ggcgcaggcg cttaatctcc aggatcgcgg ggtgattggc
    10321 gagggcaaac gcgccgacct ggtgctggcg catcgcaagg gcaatcacat tcatatcgac
    10381 cacgtctggc gtcagggtaa aagggtgttc tgatgatggg aaaactgatt tggttaatgg
    10441 ggccgtccgg ctccgggaaa gatagcctgc tggcggaact ccgcctgcgg gaacaaactc
    10501 agctactggt ggcgcatcgc tacatcacgc gcgacgccag cgccggaagc gaaaaccata
    10561 tcgccctgag cgagcaggaa ttttttaccc gcgcggggca aaatctgctg gccttaagct
    10621 ggcacgctaa cggtctgtat tatggcgtcg gcgtcgagat tgacctctgg ctgcacgctg
    10681 gattcgacgt gctggtcaac ggctcacgcg cccatctgcc gcaggcgcgg gcgcgctatc
    10741 aatcggcgct gctgcccatt tgcttacagg tttcgccaga gatcctgcgc cagcgcctgg
    10801 aaaaccgtgg ccgcgaaaac gccagtgaaa ttaacgcccg gctggcgcgc gccgcccgct
    10861 atactccaca ggattgccat acgctcaata atgacggcag cctgcgccag tcggtcgaca
    10921 cgctgctgac gctaatccat cagaaggaga aacaccatgc ctgcttgtga gctttcgccc
    10981 ggccacgcag tacgacaccg acgcggttta cgcgttgatt tgcgagctaa aacaggcgga
    11041 gtttgaccac cacgcgtttc gcgtgggctt taacgccaac ctgcgcgacc cgaacatgcg
    11101 ctaccatctg gcgctgctgg atggcgaagt tgtcggtatg atcggcctgc atttgcagtt
    11161 tcatctgcat catgtcaact ggatcggcga aattcaggag ttggtggtaa tgccgcaggc
    11221 gcgcggtctg aacgtcggca gtaagttact ggcgtgggca gaagaagaag cccgccaggc
    11281 cggggccgaa atgaccgaac tttcgaccaa cgtgaagcgc cacgacgcgc accgtttcta
    11341 tctgcgcgaa ggctacgagc agagccactt ccgcttcacc aaggtgctgt aacatgagcc
    11401 tgaccctcac gctcaccggc tccggcggcg cacagggcgt tccggcatgg ggctgcgagt
    11461 gtgcggcctg cgccagagcg cggcgctcgc cgcactatcg ccgccagcca tgcagcggcg
    11521 tagtgaagtt taacgaggcg atcaccctga tcgacgccgg gcggcacgat ctcgccgatc
    11581 gctggtcgcc cggatcgttc cagcagtttt tgctgacgca ctaccatatg gatcacgtgc
    11641 aggggctatt cccgctgcgc tggggcgtgg gcgatccgat cccggtttac ggcccaccgg
    11701 atgaacaggg ctgcgacgat ctgtttaaac atccgggcct gcttgatttc agccacacgg
    11761 tggaaccgtt tgtggtgttt gatttgcagg ggttacaggt cacacccctg ccgctcaacc
    11821 actcaaaact gaccttcggt tatctgctgg aaacggcgca cagccgggtg gcgtggctgt
    11881 ctgacaccgc tggcttgccc gaaaaaacgc tgaaattttt acgcaataat cagccgcagg
    11941 taatggtgat tgattgcagc cacccgccgc gcgaggatgc accgcgtaat cactgtgatt
    12001 taaataccgt gcttgcgctg aatcaggtta tccgctcgcc gcgcgtggtt ctgacccata
    12061 tcagccacca gtttgatgcg tggctgatgg aaaacgcact gccgtcgggg tttgaggcgg
    12121 ggtttgatgg gatggagatt ggggtggcgt gatgagaggg aatgtgcgtg ctggccccct
    12181 caccctaacc ctctccccag aggggcgatg cctgccccat acccaacatc attgcccata
    12241 ctcatcttcc attctccgct cttcatcttc tagctgccga cgctcctgat taagctggtg
    12301 ctggcgatcg tccagctgcc tgcgacgatc ttcaaactgg cgacggcggt cgtcatattg
    12361 tctgcgccga tcgtcgctca cttcacgctg ccagccgtcg tcgcgcgaat cttcataatc
    12421 tcgcccacgg tcagggttag aagcgtcgtt aatcgcctgc tgaatattgc caatggtgtc
    12481 atcgataata tcggcctggg ccggtacggt ggtcagcgtg agcagggtga aaagaaaaag
    12541 cgtggagtgg cgtttcataa actcggcctc gcaacaaagt taatacaagg taggcaaagg
    12601 tgcctgttac gagtagagga ggattctcaa ttctcttgcc ccagcgcctg cgccaactgc
    12661 gcacgggtaa agggtttgcg caacagcgcc acgtctggca gcgcggggtt atggctgggg
    12721 cgtaaatcct gaccgctgat gagtagcagc gtcaggtgcg ggtaaagttt gcgggccgca
    12781 ttgaccactt ctgcgccgct catgccgccg gggagcatta aatcgctgat aaaaatatcg
    12841 atttctgctg atgccgccag cagatccagc gcctgttcgc cgctgcttgc ttccagcgtc
    12901 aaatagccca gcaggtgcaa ctgttcgcag atggtctggc gcaccgcggc ttcatcttcc
    12961 agcactaaca ccagtttatc gccgctgaca gaagcctgtt ctactgccgc tggcgatagg
    13021 tttttcgccg ccgtcagcgc gcggggaagc tgtaatctga ccgttgtccc ctgccccggc
    13081 gcgctttcaa tttcaacgcg cccaccggac tggcgcacaa aaccatagac catcgacaaa
    13141 cccagcccgc tgccgctgcc cgtctgtttg gtggtaaaga atggctcaaa tacctggctt
    13201 ttcaccgcct gcgacatacc gcagccatga tcgatgacct ccagtgccac catgtcctga
    13261 cgctgcccac tgctgcgagt aacgcgctga ttccaggtac gaattttgat gattcccgtc
    13321 tgcccttcca tcgcgtcacg ggcgttcatc accaggttga tgatggcatt ttccagttgg
    13381 ctgatgtcaa tccacgcggg ccacgccgga gactgcgctt caatctccag cgccagcgtt
    13441 gccggtaagg agtggcgcat taattcgctt aactcttcca gcaatgtttt cagttcaacg
    13501 gcttgcggat gtaaggactg cttgcgggaa aacgccagca gtcgttgtgt cagtagcgcc
    13561 ccacgttcgg cagcttgcag cgcccgctgt attcgcggcg cgtctggcga gtccggattc
    13621 accagctcca ggctgccaat aatcaccgcc agcagattat taaaatcatg ggccagcccg
    13681 cccgtcagtt gcccgacagc tttcagtttc tggctgtgta atagcgcctc ttccaacccc
    13741 tgccgctcag tttgggcaat ctccatctgc gtggtcttct cttttagcag ccgtgaggtt
    13801 cgttcgagtg acgcggtatt gcgggcaaaa acgttaaagg cgcgcgccag ttcacccaac
    13861 tcatcacgcc gctgtaacgc cggaaaagtg acgtccgctt cgccgtgcgc caggcggctc
    13921 atggcgcggg aaatcgccgt caggttagtg ccgaggttgc gatagatata ccagccggca
    13981 aacgcggtga tcaccaccgc taacagggcg aaaatcatga tcaccctctc gccggagcgc
    14041 aaatcctggt aactttgcgc cacacgctga ccggagagcg tcgccacctg ttcggcgtaa
    14101 cggttgatgt cgccattgag gatcgccacc agcgctttga tgtgaaacat ataccagctg
    14161 attgccagat cgctttgctc cagagcggca gaaagcggcg cgagtttacg catttcatca
    14221 ttgaaatcat tgaaatcatt gagtaacgta ttaaccaatg gcgtatcgct gtgggtcggc
    14281 aacgcagaca ttaccccgac cagttgatga ataatcgcct gcggcgcggg ggtggcaata
    14341 gcggcaacaa tcagtcggtt catctcgtta agcaaaatat catcttgcgc gtgagtgact
    14401 ttttgcagat gccgcagata acttaagttt tgatacagcg aacttaacag cgcgttgcgc
    14461 tccagatgcc gcctttgccc gcgctccagc ataccgccga cgctggtttg cagttcgttg
    14521 ctacgcgtga tgattcgcgt caccagcccg gcttcctgct gcgctaaagg cgcgttcgcc
    14581 agttgtgcaa gcgagctttt taatgcccgc tgtgtctcct gcaaccgctc ggcttcgcct
    14641 ttatattcca gcgctcccac cacctgcgac aatcgcaccg ccgccgttgc cacgtttgcc
    14701 gtgtcgcgcg ccagattcag gctgccggtc atatcatcca ccgtttgctg ctgcaccagc
    14761 tcctgcatct ggctggcatg acgaaagccc attaccgcca cgccgctgac catcaacgtc
    14821 accgccacca ccagcaaatt caaaagcagc aatcgcccgc gcgcgctggc aaaaaagtga
    14881 cgtccggggt gcgccatatc acgctccgtg aagaaaaatc gcaatatgac aaatatgaac
    14941 ggcttctgac attttgcatt tactttcctg tgatggagtg ctgatatcga caacctcgca
    15001 ggagtccacc atgaacagca cgccagttct ggagatgcgc aatattgcca aagccttcgg
    15061 caaattttat gcactcaaag gggtggattt gacggtctac cctggcgaga tccatgccct
    15121 gatgggggaa aacggcgcgg gaaaaagcac gctgatgaag gtgctggcgg gggcgtatac
    15181 cgccaccagc ggcgagattc tcatagacgg caagcccttt cacattcgca cgccaaaaga
    15241 tgccttaagc gccggtatta cgctgattta tcaggagatg cagctggcac cgaatctgtc
    15301 ggtggcagaa aatatttttc tcggcagcga gctttcccac ggcgggctgg tgcagcgtaa
    15361 agagatgcta gtgcaggcgc aaaaagtgat cgaccgcctc ggcgcgcagt ttaacgccag
    15421 cgataaggtc atgacgctga ccattgccga gcaacagcag gtcgaaatcg cccgcgcact
    15481 acatcgcaac agccgcattc tggtgatgga cgaacccacc gctgccctct cctcccgcga
    15541 aactcaccgc ctgtttgaac tgattatgcg gctgcgcgat gaagggatgg cgattatcta
    15601 cattagccac cgcatggcgg aagtgtatga gctttccgat cgcgtcagcg tgctacgcga
    15661 cgggcaatac gttggcagcc tgacccgcga taacctcaat gccggggagc tggtgcggat
    15721 gatggtcggc aggccactga gcgatctgtt caataaagag cgcgatatcc cgctcggtaa
    15781 agcccgcctg aatgttcacc acctgaccga cggcggcaaa gtccagccga gtagcctgct
    15841 ggtgcgttcc ggcgaaattg ttggcctcgc cggactggtg ggtgccggac gttccgaact
    15901 ggcgcagttg atcttcggcg tgcggaaagc gacaggcgga atgattgaag tcgatggtga
    15961 accggtggtg atccactccc cgcgcgaagc catcgatctt ggcattggtt ttctcaccga
    16021 aaaccgcaaa gaacaaggct tattccttga aatggcagca gccgaaaaca tcaccatggc
    16081 aaccctggag cgcgatgccc gctggggaat gctcaatcgc aaaaaagcgc aaaccatttc
    16141 cgatgacgcc attaagttgc tcaacattcg cgtgcctcat gcccaggtac gcgcgggcgg
    16201 gctttccggc ggcaatcagc aaaaactgtt gatctcccgc tgggtggcga ttggcccgcg
    16261 cattttactg ctcgatgaac ccgcccgcgg cgtggacgtt ggcgccaaaa gtgagatcta
    16321 ccggatcatg aacgagatgg cgcgcaaggg cgtggcgatc ctgatgatct ccagcgaact
    16381 gccggagata gtgggaatga gcgatcgcgt ctatgtgatg cgcgaaggca gcattgccgg
    16441 tgagttaaac ggcaaaaaca tcacccagga aaacattatg accttagcga ctggcgtcaa
    16501 cgacgcccat tcccaggcgg taacctcatg acgacctctg caaaaacctc tgccaaaaaa
    16561 atgctgatga gcgatctgat gcaaacggtc ggcatcttgc ccattttgat tctgatcgtg
    16621 gcggtatttg gctttatcac gccaaacttt tttaccgaaa gcaacctgct gaatattacc
    16681 cgccaggcat cgatcaacat tgttctggcg gcggggatga ccttcatcat tttgaccggc
    16741 gggatcgatc tttccgtggg ttcgatcctc ggcaccacag cggtggcggc gatggtcgtc
    16801 tcccttagcc ctgagttcgc catgctgtcg atcccggcag cgctgatgct cggcctgctg
    16861 ctggggctgt tcaacggcgc gctggtggcc tttgccggat tgccaccgtt tatcgttacg
    16921 cttggcacct atacggcgct gcgcggggcg gcgtatctgc tggcggacgg caccacggtt
    16981 atcaactcca acattaattt cgagtggata ggcaacaact atctcgggcc ggtcccatgg
    17041 ctggtggtga ttgcgctggc ggtgatcgtg gtgtgctggt ttattctgcg ccgcaccacg
    17101 cttggcgttc atatctacgc cgtcggcggc aatatgcagg cggcgcgtct taccggcatt
    17161 aaggtctggc tggtgctgct gtttgtttat ggcatgagcg gcctgctttc tgggcttggt
    17221 gggattatga gcgcctcacg gctgtacagc gccaacggca acttaggcat gggttacgaa
    17281 ctggatgcca ttgcagcggt gatcctcggc ggcaccagtt ttgtcggcgg catcggcacc
    17341 atcaccggca cgctggtcgg cgcgctaatt atcgccacgc tcaacaatgg catgacctta
    17401 atgggcgtct cttacttctg gcaactggtg atcaaagggg cgatgatcat cattgcggtg
    17461 ctgatcgaca aataccgtac ccgacaccat caaagtgcat aaacacatga ggaatacagc
    17521 atgagattga agcccttagt gaccgcgctc tgtgcaggcg cgctgctggc cgcagcaccg
    17581 ttcgcccagg cgaaagaact gaaatcaata ggcgtaacgg tgggcgatct ggcgaacccg
    17641 ttcttcgtgc agatcaccaa aggcgcggag ctggaagcac gcaaactggc aggcgataac
    17701 gtcaaagtga cgctggtttc cagcggctac gatctgggtc agcaggtgtc gcagatagac
    17761 aactttatcg ccgctaacgt cgatatgatt atcctcaacg ctgccgattc aaaagggatc
    17821 ggcccggcgg tgaaacgggc gaaagatgcc gggatcgtgg tcgtcgcggt tgacgtagcg
    17881 gcagaaggtg ccgatgccac catcacttcc gataacaccc aggcaggcga aatggcctgt
    17941 aaatacatca ccgatcgcct gaaaggcaaa ggcaacgtgg tgatcatcaa cggcccgccg
    18001 gtatccgccg tgcaaaaccg cgtcgaagga tgccagactg aatttaaacg ccatcctgac
    18061 atcaaagtgc tctctgacaa ccagaacgcg aaaggcagtc gtgaaggcgg gctggaggta
    18121 atgacgggac tgctggcggc aaatccgaag atcgacggcg tatttgcgat taacgatccc
    18181 accgcaatcg gtgccgatct ggcagcaaaa caggcacaac gtaacgagtt ctttattgtc
    18241 ggtgtggacg gaagcccgga cgccgaagag gcgctcaagc gtgagaactc gttgtttgtc
    18301 gctactccgg cgcaagatcc ccaggtgatg gcagcgaaag cggtggaaat tggctacgac
    18361 attttgcagg gcaaacctgc gccgaaagag ccggtgctga tcccggtgac gatgatcgat
    18421 aaaaacaacg tcggcaatta caaaggctgg acggtgaaat aagtccacac gccgggcggc
    18481 gcagctgccc ggtcaccgct aaggagaacg ctttcgatga aacgctccgc tataaacgac
    18541 attctcggcc atacacggca attcttttcg caacatgacg tacatctgcc gccctttgcc
    18601 agtttctcgc ctgctcaatg gcagcagctc gacaccgccg cgtgggagga ggtttttgat
    18661 cttaaactcg gctgggatgt taccgctttt ggtcgcaaca actttgcagc acacgggctg
    18721 acgctgttta ccctacgtaa cggctcagca aaaggaatgc cgtatgtgaa atgttacgcc
    18781 gagaagatca tgcatgtgcg cgatgcgcag gtcacgccaa tgcattttca ctggcgtaag
    18841 cgtgaggaca ttatcaatcg cggcggcggt aatttaattg ttgaactgtg gaatgccgat
    18901 agtaacgagc aaacggctga tagtgacatt acggtggtga tagacggctg ccgccagaaa
    18961 catactgcag gctctcagtt gcgcctgtct cccggcgaaa gtatctgcct gccgcccggc
    19021 ctgtatcaca gcttttgggc agaagccggt tttggcgatg tgctggttgg cgaagtctct
    19081 tctgttaatg acgacgacca cgataaccac tttttacagc cactggaccg ctacaacctg
    19141 attgacgaag acgaacctgc acagttggta ttgtgtaacg aataccgcca gttccgctga
    19201 ataaggataa cgttatgcca ttgatttctc tcgctgaagg gctggcacat gcccgcgaac
    19261 atcactattc gttaggtgcg tttaatgtgc tggactcgca ctttttacgc gccttgtttg
    19321 ctgctgccag acaagaacgt tcgcccttta ttatcaacat tgccgaagta cattttaaat
    19381 atatctcgct ggattccctg gtcgagacag tgaaatttga agcggcgcgt catgacattc
    19441 cggtggtgtt aaatctcgat cacggactac attttgaatc ggtggtgcaa gcgttgcggc
    19501 tgggattcag ctcggtaatg tttgatggct ccacgctggg gtatgaagaa aatgtccgcc
    19561 agacgcggga agtggtgaaa atgtgccatg ccgttggcgt atcggtagaa gctgaactgg
    19621 gcgcggtggg tggcgacgaa ggcggtgcac tctatggtca tgccgatgaa tccctgttta
    19681 ccgatccgca actggcgcgt gattttgtcg accgtaccgg cattgacgcg ctggccgtgg
    19741 cgattggcaa tgcacacggc aaatatcgcg gcgaaccgaa actcgatttc ccgcgcctcg
    19801 acgctatccg ccagcaggct gctatcccgc tggtattaca cggcggctca gggattagcg
    19861 atgccgattt ccgccgcgcc atcgaactgg gtatccacaa aatcaatttc tacactggca
    19921 tgtcgcaagc ggcgctggct gccgttgagg cccgaatggc gcaccgtcag ccgatgtatg
    19981 acgagtttgc cgagttactg ctggcagtgg aagccgccat cagcgatacg gttgcacaac
    20041 agatgcgtat ttttggcagt gcggggcagg catgatggag cgtaaaggga ttatcgccgc
    20101 aggcaatatg ttggttgacc acgtccatca gattatccag tggccagagc gcggctggct
    20161 ggcggagatc acccacagcg aacgctccac cggcggtgcg ccgcttaacg tcctgctgac
    20221 gctggcgaaa atgcgcaccg ggctgccgtt acaggcggtt gggctaatcg gtcaggacag
    20281 cgacggcgat tacatcatgg cgatgcttga gcaatatcac gtcaatcgtc agcatgtgca
    20341 gcgcactacc tttgccccga cgtcgatgtc gcaggtgatg acagacccaa gcgggcagcg
    20401 gacgtttttc cattcgccgg gtgcaaacag acttttggat cttccggcgt tcgatcagct
    20461 cgataacacc atgaagattt tccatctcgg ctatttactg ttgctcgaca gcctggatat
    20521 gccggacgat gtttacggca cccgtagcgc acgcctgctg gcgcaaatgc gtgatactgg
    20581 ctttgaaacc tcactcgacc tggtctcccg taagggcgat ccgcgttatc agccgctggt
    20641 gctgcccgcg ttgcgccatc tggattatct ggtaattaac gaactggagg ccggggagtt
    20701 tagcgggctg gaaattcggt tacccaacgg cgaaccgcat atcgcgcata ttgctgccgc
    20761 cgcgcgtgaa ctgttagatg cgggcgtgcg ccagcgggtg gtgatccact acccggaagg
    20821 ggcgtgggga gagaccccag aacagggcgg cgtgtggatc ccttcgcaaa agttagaaca
    20881 acgggaaatt atcggcagcg tcggcgcggg cgatgcattt tgtgccggat ttttatatgg
    20941 ctgccatgaa cgctggacgc ttaccgagag catcaaactg gcacacgcct gcgcgcgggc
    21001 aagcctgtta tgcgccaatg ccatcgacgg ggcgaaaacg ctggaagagc tgcaaacaga
    21061 aatgtcggat taatgcacgt cggcagcgaa gacatacccc agaccgcgca gagttttaat
    21121 caacatcggc tgatgcgggt tgagttcgat tttgcgtcgt aagcgcatga ttaatacgtc
    21181 gatggtgcga tcaaacacct ccacgctgtc gctgtgggtg aacgccagca actgctcgcg
    21241 gctcaatacc cgacgcgcgt tttgcgccag agccagtaac aagccatatt cgccctgagt
    21301 taactcgaca tgttgctgaa gcggattgtg caactggcag cgcatggtat cgagatacca
    21361 gccattgaat gacagcccgg cgcttttctc gtcattaacc gccagcgcgc caacccgtcg
    21421 caacaccgct ttcagtcgtg cgaccaccac ccgcggggtg aacggtttgc agatgtaatc
    21481 atccgcgccc atttccagcc cgaccaccac atcagactca ctgcccatgc cggtcaacat
    21541 caccaccggc aatgtcggac gcagtttctg gatttgctgc aataccctca ggccgttggt
    21601 atcgggtagc atcatatcca gcaccaccag cgcaatgtca ggatcgccct caatacgcaa
    21661 tatggcctcc tgtccggtat ggcagactga aaccgtaaag acatgttcac ttaacacgtc
    21721 ctgcaacagt gcgcagatag ccgtgtcatc atccaccacc agcacgacgg gtttcatggt
    21781 tacactccgc atcattcatc tgattaatca ttgtgcgtac ttcttgtcga agtgaaagct
    21841 gaaagtaata taaataataa aaatataagt caggtcacat tattgatgat agtaataaat
    21901 atgtcgtact tttccctctt cagcacaaat gcctaataaa atggatcgtt ccgctcacac
    21961 ttccacttat taaagtcgca atttcccaat tatgataacg ccagctttaa atgacgtatt
    22021 ttgcatggat tacatattta cgacataaat gataaatgga gatttatatg aataactctc
    22081 ggttattccg tttgagcagg attgttattg cgttaactgc cgccagcggc atgatggtaa
    22141 ataccgctaa cgcgaaagag gaagcgaaag ccgccactca atatacccaa caggttaatc
    22201 agaattacgc caaatcatta ccgtttagcg atcgtcagga ttttgacgat gcccagcgtg
    22261 gatttatcgc cccgctgctg gatgaaggta ttctgcgtga tgcgaacggt aaagtttact
    22321 accgcgcaga cgattacaaa tttgatatta atgccgctgc gccggaaacc gtaaacccca
    22381 gcctgtggcg tcagtcgcag atcaacggca tttctggcct gttcaaagtc accgataaaa
    22441 tgtatcaggt gcgcggccag gatatctcta acattacgtt cgttgagggg gagaaaggca
    22501 ttattgtcat cgacccgctg gtgacgccgc ctgccgcaaa agccgcactt gacctttact
    22561 tccagcatcg tccgcaaaaa ccgattgttg ccgttatcta cactcacagc cacaccgacc
    22621 actatggtgg cgtgaaaggc attatctctg aagccgatgt taaatccggc aaagttcagg
    22681 tgattgcccc tgctggcttt atggacgaag ccatcagcga aaacgtgctg gcgggtaaca
    22741 tcatgagccg ccgtgcgctc tactcttacg gtctgttact gccgcacaac gcgcaaggca
    22801 acgtgggtaa tggccttggc gtgacgctgg caacgggcga cccgagcatt attgcaccga
    22861 ctaaaactat cgtcagaact ggcgagaaga tgattatcga cggcctggag tttgacttcc
    22921 tgatgacccc aggtagcgaa gcgccagccg aaatgcactt ctatattccg gccctgaaag
    22981 ccctgtgtac cgccgagaac gccacgcata ccctgcacaa cttctacact ctgcgcggcg
    23041 cgaaaacccg cgataccagc aagtggaccg agtacctgaa cgaaacgctg gatatgtggg
    23101 gtaacgacgc ggaagtactg tttatgccgc acacatggcc ggtctggggc aataagcata
    23161 tcaatgatta tattggtaaa tatcgcgata ctatcacgtt cattcacgac cagaccctgc
    23221 acctggcgaa ccagggctac accatgaatg aaatcggcga catgattaag ctgccgcctg
    23281 cacttgccaa taactgggcc agccgtggct attacggttc tgtcagccac aacgcccgcg
    23341 cggtgtataa cttctatctt ggctattacg acggtaaccc ggctaacctg catccgtatg
    23401 gtcaggtaga gatgggcaaa cgttacgtga aagcgctggg cggctctgcc cgtgtcatca
    23461 acctggcgca agaagcgaat aagcaaggtg attaccgctg gtcggcagaa ctactgaaac
    23521 aggtgattgc cgccaacccg ggtgaccagg tggcgaagaa tctgcaagcg aataactttg
    23581 aacagctggg ctatcaggcc gagtccgcca catggcgcgg tttctacctg accggcgcga
    23641 aagagctgcg cgaaggggtg cataagttca gccacggcac caccggttcc ccggacacca
    23701 ttcgcggaat gtcggtcgaa atgctgttcg actttatggc cgttcgcctc gatagcgcga
    23761 aagctgcggg taaaaatatc agcctgaact tcaatatgag caacggcgat aacctcaacc
    23821 tgacgctgaa cgatagcgtg cttaactacc gtaaaacact gcaaccgcaa gccgacgcct
    23881 ctttctacat cagccgtgaa gatctgcacg ccgtgctgac cggacaggca aaaatggcgg
    23941 atctggtgaa agcgaagaaa gccaaaatta ttggcaatgg cgcgaaactg gaagaaatta
    24001 tcgcctgtct ggataatttc gatttgtggg tgaatatcgt aaccccaaat taatgtcact
    24061 cg
//
LOCUS       DAZKIF010000072        23994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZKIF010000072 DAZKIF010000000
VERSION     DAZKIF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.072"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            115..3867
                     /gene="yfaL"
                     /locus_tag="ACHOTW_002798"
     CDS             115..3867
                     /gene="yfaL"
                     /locus_tag="ACHOTW_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP9844873.1"
                     /translation="MRVIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4008..4730)
                     /gene="ubiG"
                     /locus_tag="ACHOTW_002799"
     CDS             complement(4008..4730)
                     /gene="ubiG"
                     /locus_tag="ACHOTW_002799"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP9844874.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4877..7504
                     /gene="gyrA"
                     /locus_tag="ACHOTW_002800"
     CDS             4877..7504
                     /gene="gyrA"
                     /locus_tag="ACHOTW_002800"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP9844875.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7712..9340
                     /gene="yfaA"
                     /locus_tag="ACHOTW_002801"
     CDS             7712..9340
                     /gene="yfaA"
                     /locus_tag="ACHOTW_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP9844876.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            9337..9960
                     /gene="yfaT"
                     /locus_tag="ACHOTW_002802"
     CDS             9337..9960
                     /gene="yfaT"
                     /locus_tag="ACHOTW_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFP9844877.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9984..14498
                     /locus_tag="ACHOTW_002803"
     CDS             9984..14498
                     /locus_tag="ACHOTW_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP9844878.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEESKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAVNSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPSVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREAAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14499..16148
                     /gene="yfaQ"
                     /locus_tag="ACHOTW_002804"
                     /pseudo
     CDS             14499..16148
                     /gene="yfaQ"
                     /locus_tag="ACHOTW_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            16153..16929
                     /gene="yfaP"
                     /locus_tag="ACHOTW_002805"
     CDS             16153..16929
                     /gene="yfaP"
                     /locus_tag="ACHOTW_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP9844879.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17204..20053
                     /gene="rcsC"
                     /locus_tag="ACHOTW_002806"
     CDS             17204..20053
                     /gene="rcsC"
                     /locus_tag="ACHOTW_002806"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP9844880.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20253..20903)
                     /gene="rcsB"
                     /locus_tag="ACHOTW_002807"
     CDS             complement(20253..20903)
                     /gene="rcsB"
                     /locus_tag="ACHOTW_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP9844881.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20920..23592)
                     /gene="rcsD"
                     /locus_tag="ACHOTW_002808"
     CDS             complement(20920..23592)
                     /gene="rcsD"
                     /locus_tag="ACHOTW_002808"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP9844882.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5871 a         5844 c         6532 g         5747 t
ORIGIN      
        1 aataataaca aaatagctat tgcatcgggt tggacaattt acaaaagaaa gataacccat
       61 attgtataat gcgtttaaat atgccgtctt atataaaatg atggtcagat taatatgcgg
      121 gttatctttc tacgcaagga gtatttatct ctactcccgt caatgattgc atctcttttc
      181 tctgctaacg gtgtcgcggc ggccattgat ttatgccagg gatatgatat caaagcgagt
      241 tgtcacgcca gcaggcaaag cctttcaggc attacgcagg tctggagtat tgccgatggg
      301 caatggctgg ttttttcgga tatgaccaat aatgccagcg gtggggccgt atttttgcaa
      361 caaggagcgg aatttacatt atcaccagaa aatgaaactg gaatgactct gtttgccaat
      421 aacaccgttt caggagaata taataacggc ggggcaatat ttgctaaaga aaactcaacg
      481 ctgaatctta cggatgttat tttttctggt aacgtcgcag gcggctatgg tggcgcaatc
      541 tattcttctg gtactaacga taccggtgcc atcgatttac gtgtcactaa cgccgtgttt
      601 cgcaataaca tcgctaatga cggcaaaggt ggtgcaattt ataccatcaa taatgatatc
      661 tatttaagtg atgatgtttt taacaataac caggcatata catcaacaag ttacagtgat
      721 ggcgatggcg gcgcaatcga tgtcacagat aataatagcg acagcaagca tccttcaggt
      781 tatacgataa taaataacac tgcctttaca aataacactg ccgaaggtta tggcggggcg
      841 atatatacca atagcgcgac ggctccctat cttattgata tttctgttga tgacagctac
      901 agccagaacg gaggcgtgtt agtcgatgag aacaatagcg cagcaggcta tggagatggt
      961 ccttcctctg cggcgggtgg ctttatgtat ctcggcttaa gtgaagttac ctttgatatt
     1021 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
     1081 gctggtaccg ggttaatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
     1141 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
     1201 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
     1261 acgataggga gtattgataa gtaccagaat caggcagagc taaatgttgg ctccacccaa
     1321 caaacctttg cgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
     1381 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
     1441 accattgcgc aaaacggcag ctatgtgctg gcgggggcgc agtcgatggc gctaaccggc
     1501 gatatagtgg tggatgctgg tgcggtgctt tcgctggaag gcgacgcggc agatcttgcc
     1561 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtatgctcga tctctctgat
     1621 ttctccacct ggcagagcgg tacatcatac aaagatggcc ttgaagtcag tggcagcagc
     1681 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gcggaaacga tatgcatatc
     1741 ggcggcgacg ggaaagatgg cgtctacgtg gtgatcgatg cgggtgacgg gcaggtcagc
     1801 ctggcaaatg acaatcaata cctcggcaca acgcaaatcg cttccggtac gctgatggtg
     1861 agcgacaact cgcagcttgg atatacccat tataaccgcc aggttatctt taccgataag
     1921 ccacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac aacgactgag
     1981 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     2041 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     2101 ttgactaaaa cgggggcggg tacactggag ctgaccgcca gcggtacaac gcagtcagcg
     2161 gtgagagtag aagagggcac gctgcaaggt gatgttgcgg atatcttccc ttatgcttcg
     2221 tcgctatggg tcggtgacgg ggcaacgttc gttactggcg cggatcagga tattcagtca
     2281 attgatgcta cttccagcgg cactatcgac atcagcgatg gtacggtttt gcgcctgacc
     2341 gggcaggata cttccgtcgc ccttaatgcc tcactgttta actgcgatgg gacgctggtg
     2401 aatgccaccg atggtgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     2461 ctgacttatc tttccaacgt gacggttaat ggcaatctga ccaatacgtc cggtgcggtt
     2521 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     2581 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     2641 atgaacggta atactgctgg caacacaact gtggtggtta actccattac agggattggt
     2701 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     2761 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     2821 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     2881 ccacctgatc cagacccgac tcccgatcct gatcccacgc aggatcctga tccaacaccc
     2941 gacccggaac ctacgcctgc ttaccagccg gtgttgaatg ccaaagttgg cggttatctc
     3001 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgcaggtggc
     3061 gatggtcaga cgctgaattt acgtgttatc ggcggagatt atcattacac agcagcgggg
     3121 caactggctc aacatgaaga cacttctacg gtgcaactta gcggcgatct gtttagcggg
     3181 cgctggggca cggatggcga gtggatgctt gggattgttg gtggctacag cgataaccag
     3241 ggcgacagcc gctcgagtat gaccggaact cgcgccgata accagaacca cggttatgcg
     3301 gttgggctga cctcaagctg gtttcagcac ggtaagcaga agcagggggc ctggctggat
     3361 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcgtggat
     3421 cattaccatt cgtcggggat tatcgcctcg ctggaagcgg ggtatcagtg gttaccgggg
     3481 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     3541 tttaccgccg ctaaccgtgc gcgcgtgtca caatcgcagg gtgatgatat tcagacgcgg
     3601 ctgggtttac acagcgaatg gcgtaccgct gttcatgtca taccaacatt agatctgaat
     3661 tattatcacg atccccattc gacggaaatt gaagaagatg ccagcactat cagtgacgat
     3721 gcggtgaagc aacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtt
     3781 tcgctgcgtg gtagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     3841 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcactattg ctggatgcgg
     3901 cgtaaacgcc taatcaggcc tacggagagc atttgtaggc ctgataagac gcggcaagcg
     3961 tcgcatcagg cattggcgcg gttgccggat gaatccaaac aacaacctca cttattctgc
     4021 gtgtgcagca tatagttcac atccacgccg gggccgagtt taaaagtatt agtgatcggg
     4081 ttgtaatgta gcccggttat atgtcgctct ttaaggcttg tctgatctac ccagcccagc
     4141 agttctgccg gtttaataaa cttcttcaca tcatgtgtac ctttaggcac catgcgcaaa
     4201 atatattccg cgccgactac cgccatcagc catgacttgc cgttgcggtt aagcgtcgag
     4261 aaaaagacat cgccgcccgg tttcaccagt tgcgcacagg ctctgaccac tgactgcgga
     4321 tcagggacgt gctccagcat ctccatgcag gtcaccacat catactgccc ggcatgtttt
     4381 gccgcgtgct cttccacggt ttcctgcacg taatccacct gaatgccgct ttccagcgcg
     4441 tgcagctttg ccacctgcaa tggctcaaag cccatatcca gaccggtcac cgtcgcgcct
     4501 tcgcgcgcca tactctcggc cagaatgccg ccgccacaac cgacatcgag cacctttttg
     4561 ccaaataagc cgccagcacg ctcggcaata tagctcagac gcagcgggtt aatgcggtgc
     4621 agcggtttga actcaccttc cagatcccac cagcgggagg cgacggcttc aaatttagcg
     4681 atctcttcgt ggtctacgtt atggtttacc ggcgattttt cggcattcat tggcacttct
     4741 actccgtaat tggcaagaca aacgagtata tcaggcattg gatgtgaata aagcgtatag
     4801 gtttacctca atctgcgcgg ctgtgttata atttgcgacc tttgaatccg ggatacagta
     4861 gagggatagc ggttagatga gcgaccttgc gagagaaatt acaccggtca acattgagga
     4921 agagctgaag agctcctatc tggattatgc gatgtcggtc attgttggcc gtgcgctgcc
     4981 agatgtccga gatggcctga agccggtaca ccgtcgcgta ctttacgcca tgaacgtact
     5041 aggcaatgac tggaacaaag cctataaaaa atctgcccgt gtcgttggtg acgtaatcgg
     5101 taaataccat ccccatggtg actcggcagt ttatgacacg atcgtccgta tggcgcagcc
     5161 attctcgctg cgttacatgc tggtagacgg tcagggtaac ttcggttcca tcgacggcga
     5221 ctctgcggcg gcaatgcgtt atacggaaat ccgtctggcg aaaattgccc atgaactgat
     5281 ggccgatctc gaaaaagaga cggtcgattt cgttgataac tatgacggca cggaaaaaat
     5341 tcccgacgtc atgccaacca aaattcctaa cctgctggtg aacggttctt ccggtatcgc
     5401 cgtaggtatg gcaaccaaca tcccgccgca caacctgacg gaagtcatca acggttgtct
     5461 ggcgtatatc gatgatgaag acatcagcat tgaagggctg atggaacaca tcccggggcc
     5521 ggacttcccg acggcggcaa tcattaacgg tcgtcgcggt attgaagaag cttaccgtac
     5581 cggtcgcggc aaggtatata tccgcgcccg tgctgaagtg gaagttgacg ccaaaaccgg
     5641 acgtgaaacc attatcgtcc acgaaatccc gtatcaggta aacaaagcgc gcctgatcga
     5701 gaagattgcg gaactggtaa aagaaaaacg cgtggaaggc atcagcgcgc tgcgtgacga
     5761 gtctgacaaa gacggtatgc gcatcgtgat tgaagtgaaa cgcgatgcgg tcggtgaagt
     5821 tgtgctcaac aacctctact cccagaccca gttgcaggtt tctttcggta tcaacatggt
     5881 ggcattgcac catggtcagc caaagatcat gaacctgaaa gacatcatcg cggcgtttgt
     5941 tcgtcaccgc cgtgaagtgg tgacccgtcg tactattttc gaactgcgta aagctcgcga
     6001 tcgtgctcat atccttgaag cattagccgt ggcgctggcg aacatcgacc cgatcatcga
     6061 actgatccgt catgcgccga cgcctgcaga agcgaaaact gcgctggtgg ctaatccgtg
     6121 gcagctgggc aacgttgccg cgatgctcga acgtgctggc gacgatgctg cgcgtccgga
     6181 atggctggag ccagagttcg gcgtgcgtga tggtctgtac tacctgaccg aacagcaagc
     6241 tcaggcaatt ctggatctgc gtttgcagaa actgaccggc cttgagcacg aaaaactgct
     6301 cgacgaatac aaagagctgc tggatcagat cgcggaactg ctgcgtattc ttggtagcgc
     6361 cgatcgtctg atggaagtga tccgtgaaga gctggagctg gttcgtgaac agttcggtga
     6421 caaacgtcgt actgaaatca ccgccaacag cgcagacatc aacctggaag atctgatcac
     6481 ccaggaagat gtggtcgtga cgctctctca ccagggctac gttaaatatc agccgctttc
     6541 tgaatacgaa gcgcagcgtc gtggcgggaa aggtaaatct gccgcacgta ttaaagaaga
     6601 agactttatc gaccgactgc tggtggcgaa cacgcacgac catattctgt gcttctccag
     6661 ccgtggtcgc gtctattcga tgaaagttta ccagttgccg gaggccactc gtggcgcgcg
     6721 cggtcgtccg atcgtcaacc tgctgccgct ggagcaggac gaacgtatca ccgcgatcct
     6781 gccggtgacc gagtttgaag aaggcgtgaa agtcttcatg gcgaccgcta acggtactgt
     6841 gaagaaaacc gtcctcaccg agttcaaccg tctgcgtacc gccggtaaag tggcgatcaa
     6901 actggttgaa ggcgatgagc tgatcggcgt tgacctgacc agcggcgaag acgaagtaat
     6961 gctgttctcc gccgaaggta aagtggtgcg ctttaaagag tcttctgtcc gtgcgatggg
     7021 ctgcaacacc accggtgttc gcggtattcg tttaggcgaa ggtgataaag tcgtctctct
     7081 gatcgtgcct cgtggcgatg gcgcaatcct caccgcaacg cagaacggtt atggtaaacg
     7141 taccgcagtg gcggaatacc cgactaagtc gcgtgcgacg aaaggggtta tctccatcaa
     7201 ggttaccgaa cgtaacggtt tagttgttgg cgcggtacag gtagatgact gcgaccagat
     7261 catgatgatc accgatgccg gtacgctggt acgtactcgc gtttcagaaa tcagcatcgt
     7321 gggccgtaac acccagggcg tgatcctcat ccgtactgcg gaagatgaaa acgtagtggg
     7381 tctgcaacgt gttgctgaac cggttgacga ggaagatctg gataccatcg acggcagtgc
     7441 cgcggaaggg gacgatgaaa tcgctccgga agtggacgtt gacgacgagc cagaagaaga
     7501 ataattttac ttcttcatgc caaaagggag ccatctccct tttttgaatt gaaaagtcca
     7561 ggctgcaaag tctgggcttt tgtcgtatta gggcgcggta aagtttggct gtgtccgtaa
     7621 aaaatggctg gctatacaca aggaatgtgg caatgagtgg tgaaaaaaag cgaaaggctg
     7681 gcggttctat ggtcttgtag gttttggcgc aatagcactg ctttccgctg gcgtctgggc
     7741 gttgcaatat gctggcagtg ggccagaaaa aacgttgtcg ccgctggtgg tgcacaataa
     7801 tttgcaaatt gatctcaatg agccggacct ctttctcgac agcgattctc taagccagct
     7861 tcccaaagat ctcctcacca ttccgtttct ccacgatgtt ctgagtgaag atttcgtttt
     7921 ctattatcag aatcatgccg atcgtctggg cattgaaggc agcattcgtc gcattgtcta
     7981 tgaacacgat ctcacgctga aagataagct cttttcgtca ctcttagatc agcccgcgca
     8041 ggcggcgcta tggcacgata aacaaggcca tctttcacat tacatggtgc tgatccaacg
     8101 cagtggttta agcaaactgc tggagccatt gttatttgcc gctaccagcg acagccagtt
     8161 aagcaaaacg gaaatcagta gcatcaagat aaatagtgaa actattcctg tttatcagtt
     8221 gcgctataac ggcaataacg ccctgatgtt cgcgacatat caggacaaga tgctggtgtt
     8281 ttccagcacg gatatgctgt ttaaagatga tcagcaggat accgaagcca cggcgatcgc
     8341 aagtgatttg ttgagcggca aaaaacgctg gcaagccagc tttggcctgg aagagcgtgc
     8401 tgccgaaaaa acgccagtac gccagcgcat cgtggccagc gccaggttgc tgggttttgg
     8461 ctaccagcgg ttaatgcctt cttttgctgg cgtacgcttc gaaatgggta acgacggctg
     8521 gcacagtttt gtggcgttaa atgatgaatc cgccagcgta gatgccagtt tcgattttac
     8581 gccggtctgg aacagtatgc ctgccggggc cagcttctgt gtggcggtgc cgtattcaca
     8641 cggtattgcc gaagagatgc tttcgcacat cagccaggaa aacgacaagt tgaatggggc
     8701 gttggacggt gccgcggggc tgtgctggta tgaagactca aaattgcaaa ccccgctgtt
     8761 tgtcggtcag tttgatggca ctgccgaaca ggcgcaattg ccagggaaac tgtttacgca
     8821 aaatattggt gcgcacgaaa gcaaagcgcc agaaggtgtt ttgccggtaa gccagactca
     8881 gcagggcgaa gcgcaaatct ggcgtcgcga agtgagttcc cgatacggtc agtatccgaa
     8941 agcgcaggcg gcgcaacccg atcaattaat gtcggattat tttttccgcg tgtcgctggc
     9001 gatgcaaaac aaaacgctgc ttttctccct cgatgacacg ctggttaata acgccctgca
     9061 aacactgaat aaaacccgcc cggcaatggt ggatgtaata cccactgatg gcatcgttcc
     9121 gctctatatc aatccacaag gcgtggcgaa actgctgcgt aacgaaacgt tgaccagtct
     9181 gccgaagaat ctcgaaccgg ttttttataa cgccgcacaa actttattaa tgccgaagct
     9241 ggacgcttta tctcaacaac cgcgttatgt catgaagctg gcccagatgg aacccggtgc
     9301 cgcctggcag tggctaccca taatctggca gccgctatga ggcacgggct gctggcgctg
     9361 atttgctggc tgtgttgtgt tgttgcccat agcgaaatgc tgaatgtcga acaatccggg
     9421 ctgtttcgcg cctggtttgt acgcattgca caagaacagc tccgccaggg gccaagtcca
     9481 cgctggtatc agcaggattg tgcgggcctg gtgcgatttg cggcgaacga gacgctgaaa
     9541 gttcacgaca gtaaatggct aaaaagtaac ggtttatcta gccagtattt gccgccagag
     9601 atgacgctaa cacctgaaca gcgtcaactg gcgcaaaact ggaatcaggg gaacgggaaa
     9661 accggcccct acgtgaccgc gattaatttg attcagtaca acagccagtt tattggccag
     9721 gacataaacc aggcgctgcc tggcgatatg atttttttcg atcagggcga tgcccagcac
     9781 ttaatggtct ggatggggcg ttacgtcatc taccacaccg gaagcgccac gaaaactgac
     9841 aacggaatgc gcgcagtcag tctgcaacaa cttatgacat ggaaggacac ccgatggata
     9901 cccaacgatt ccaatcccaa tttcattggc atttatcgtt taaattttct ggcgcgatag
     9961 cagcgtgctt gtccctgtct cttgtgggaa cagggcttgc taatgctgat gattcgcttc
    10021 cttccagtaa ctatgcgccg cccgccgggg gaacattctt tttgcttgct gacagcagtt
    10081 ttagcagcag tgaagagtcg aaagtgcgac tggaagcgcc ggggcgtgat tatcggcgct
    10141 atcagatgga agagtacggc ggcgtggacg ttcgcctgta tcgtattcct gacccgatgg
    10201 catttttgcg ccagcagaaa aacctgcatc gcattgtggt gcaaccgcaa tatctgggcg
    10261 acgggctgaa caatacgctg acctggctgt gggataactg gtacggcaaa tctcgccgcg
    10321 tgatgcagcg tactttctct tctcagtcac ggcagaatgt gactcaggca ttacccgaat
    10381 tacagctcgg caatgccatt attaaacctt cccgttatgt acagaacaac cagttttccc
    10441 cgctgaaaaa atatcccctg gtggaacagt tccgttatcc actatggcag gctaaaccgg
    10501 tcgagccgca gcaaggggta aaactggaag gcgcatccag caatttcatc tcgccgcagc
    10561 cgggtaacat ttatattcct ctcggccaac aagagccggg actgtacctc gtcgaggcga
    10621 tggttggtgg gtatcgggcg acgacggtgg tgtttgtttc cgataccgtg gcgcttagca
    10681 aagtgtcagg caaagagctt ctggtgtgga ccgcgggtaa aaaacagggt gaagcgaagc
    10741 ccggctcaga gatcttgtgg actgacggtc ttggcgtgat gacccgcggt gtgaccgatg
    10801 acagcggtac cttgcagtta caacatatat cgccagaacg ttcatacatt ctgggtaagg
    10861 atgctgaagg cggcgttttt gtctccgaga acttcttcta cgaaagcgaa atctacaaca
    10921 cccgcttgta tatttttacc gatcgcccgc tatatcgcgc aggcgatcgt gtcgatgtta
    10981 aagtgatggg ccgcgagttc cacgatccgt tgcattcatc ccccatcgtc agcgccccgg
    11041 cgaagctttc ggtgctggac gctaacggca gtctgttgca aaccgtcgat gtcacgctgg
    11101 atgcgcgcaa tggcgggcag ggaagtttcc gcctgccaga aaatgccgtt gccggaggtt
    11161 atgagttacg tcttgcttac cgcaatcagg tctatagcag cagttttcgc gtggcaaact
    11221 acatcaagcc acatttcgag attggtttag ctctcgacaa aaaagagttc aaaactggcg
    11281 aagcggtcag cggcaaactg caacttctct acccggatgg cgagccggta aaaaatgccc
    11341 gcgtgcagtt aagtttgcgc gctcagcaat tatcaatggt cggtaacgat ttgcgttatg
    11401 ccggacgttt ccccgtgtcg ctggaaggca gcgaaacggt gtccgacgcc agcggtcatg
    11461 tggcgttaaa tctccccgcc gccgataaac cgagccgcta tttgttaacc gtctccgcca
    11521 gtgacggcgc ggcgtatcgc gtcaccacca ccaaagagat cctcattgaa cgcggcctgg
    11581 cgcattactc tttaagtacc gccgcacaat acagtaatag cggcgagtcg gttgtgttcc
    11641 gttatgccgc gctggaatct tcaaaacagg ttcctgttac gtatgaatgg ttgcgtctcg
    11701 aagaccgcac gagccatagc ggagatctac cgtcaggcgg caaatccttt accgtcaatt
    11761 tcgataaacc tggcaactac aatctgacgt tacgcgataa agacggctta attctcgccg
    11821 ggttaagcca tgccgtcagc ggtaagggca gtatgtcgca tactggtacg gtagatatcg
    11881 tggcagataa aacgctgtac cagcctggcg aaaccgcgaa gatgctgatt accttcccgg
    11941 agccaattga tgaagcatta ttgacgctgg aacgcgatcg cgttgaacag cagtcgctgc
    12001 tatcgcatcc ggcaaactgg ctgacgttac aacgtttaaa cgatactcaa tatgaagccc
    12061 gtgttccagt gagcaattcc tttgcgccta acatcacttt ttcggtgctg tatacccgta
    12121 acggtcagta cagttttcag aacgccggga tcaaagttgc cgttccccag ctggatatcc
    12181 gggtgaaaac ggacaaaacc cattaccagc ctggtgaact ggtcaatgtc gaattaacct
    12241 cgtcgctgaa aggtaaacct gtttctgcgc agctaacggt aggcgtggtc gatgaaatga
    12301 tctacgcgct gcaaccagaa atcgcgccga atatcggcaa atttttctat ccgctggggc
    12361 gtaacaatgt gcgtaccagc tccagtctgt cgtttatcag ctacgaccag gcactctcca
    12421 gcgagccggt tgcgcctggc gcaactaacc gcagcgagcg gcgagtaaaa atgcttgaac
    12481 gtccacggcg tgaagaggtg gataccgcgg catggatgcc gtcactcaca accgataaac
    12541 aaggcaaagc gtatttcacg ttcctgatgc ctgattcgtt aacccgctgg cgtatcaccg
    12601 cgcgtgggat gaacggcgac gggctggtcg ggcaggggcg tgcttatctg cgttcggaaa
    12661 aaaatctcta catgaagtgg agtatgccaa cggtgtatcg cgtgggcgac aaaccgtcgg
    12721 caggactgtt tatcttcagt cagcaggata acgaaccggt ggcgctggtg actaaatttg
    12781 caggcgctga gatgcgccag acgctgacgc tgcacaaagg ggcgaattat atttcgctgg
    12841 cgcagaacat tcagcaatct ggcttgttaa gcgcagaact gcaacaaaat gggcaagtgc
    12901 aggacagcat tagcacaaaa ctgtcttttg tggataacag ctggcccgtt gaacagcaga
    12961 aaaatgtcat gctcggcggt ggcgataacg cgctgatgtt gcccgagcag gcgagcaata
    13021 tccggctaca aagtagtgaa acgccgcagg agattttccg caacaatctt gatgcgttag
    13081 tcgatgaacc gtgggggggg gtgatcaaca ccggtagccg tctgatcccg ctcagtctcg
    13141 cctggcgttc gcttgccgat catcaaagtg ccgccgctaa cgacattcgt cagatgattc
    13201 aggataaccg tctgcggctg atgcaactgg cggggcccgg agcgcgcttt acctggtggg
    13261 gtgaagatgg caatggtgac gccttcctta cggcatgggc atggtacgcc gactggcagg
    13321 ccagccaggc gctcggcgta acgcaacaac cggaatactg gcagcatatg ctcgacagtt
    13381 atgccgagca ggcagataac atgccgttat tgcatcgggc gctggtgctg gcgtgggcac
    13441 aggagatgaa tctgccgtgc aaaacgttgt tgaaagggtt ggatgaagct atcgcccggc
    13501 gcggaactaa aactgaagat ttctctgagg aagacacccg cgatatcaat gacagcctga
    13561 tcctcgatac accggaatct ccactggcag atgcggtggc aaacgtctta accatgacgt
    13621 tgctgaaaaa agcgcagttg aagtccacgg tgatgccaca ggttcagcaa tatgcgtggg
    13681 ataaagcggt aaacagcaat cagccgctgg cacacacggt tgtgctgctc aatagcgggg
    13741 gcgacgctac ccaggcggct gctattttaa gtggtttgac cgctgagcaa tctactattg
    13801 agcgcgcgct ggccatgaac tggctggcga aatatatggc gacaatgcct tcggttgtgt
    13861 tgcctgcgcc tgcgggcgca tgggccaaac ataagttaac tggagggggc gaatactggc
    13921 gttgggttgg tcagggcgtg ccggacattc tctcttttgg tgacgaatta tcgccgcaaa
    13981 atgtgcaggt ccgctggcgt gaagcggcaa aaacggctca acaaagtaac attccggtga
    14041 ccgttgaacg ccaattgtat cggcttatcc ctggtgaaga agagatgagc tttactctgc
    14101 aaccggtgac cagcaatgag attgacagcg atgcgctgta tctcgatgaa attacgctta
    14161 ccagcgagca ggatgcagtt ctgcgctatg gtcaggtgga agtaccgctc ccgccgggag
    14221 ccgacgttga gcgcacaaca tggggcattt cagtcaataa acccaacgct ggaaaacagc
    14281 aggggcaatt gctggaaaaa gcgcgaaatg aaatgggcga actggcctat atggtgccgg
    14341 tgaaagaact gacgggaacg gtcactttcc gccatttgct gcgcttctcg caaaaagggc
    14401 aattcgttct gcctcctgct cgttatgtgc gttcctatgc acctgcacag caaagtgttg
    14461 cggcagggag cgaatggacc gggatgcagg tgaaataagt gaactggcgc agaatcgtct
    14521 ggctattggc gttggttact ctgccaacgc ttgcggagga gacgccgtta caactggcgc
    14581 tgcgcggtgc gcagcacgat caactgtatc agctgtcgtc atcaggcgtg accaaagtct
    14641 cagcattacc tgacacgctc accacgccgt tagggagttt atggaaactg tatgtttacg
    14701 cctggctgga ggatacccat caaccggagc agccctacca gtgtcgcgga aactccccgg
    14761 aagaggttta ttgttgtcag gctggggaga gcataacgcg cgataccgcg ctggtacgtt
    14821 cctgtgggct ctattttgcg ccgcagcggt tgcatattgg ggcagatgtg tgggggcaat
    14881 actggcaaca gcgtcaggca cctgcgtggt tagcgtctct ttctacgctg aagccagaaa
    14941 cctcggtaac ggttaaatcg ctgcttgatt cattagcgac gttacctgcg caaaacaaag
    15001 ctcaggaagt cttactggat gtggtgctgg acgaggcgaa aatcggtgtc gcatctatgc
    15061 tcggtagtcg ggtacgggtg aaaacctgga gctggtttgc cgatgataaa caggaaatcc
    15121 gtcagggtgg ttttgctggc tggttaaccg atggtacacc gctgtgggtg actggcagtg
    15181 gcaccagcaa aacggtactg acccgttatg cgacggtgct taatcgggtt ttgccagtgc
    15241 cgacgcaagt tgccagtggg caatgtgtag aggtggagct atttgcgcgc tagccgctga
    15301 agaaaatcac agcggagaag agtacgacgg cagtaaaacc cggcgtactt aatgggcgct
    15361 atcgtgtgac gtttgccaat ggtaaccaca ttacgtttgt cagccacggt gagacgacct
    15421 tactgaccga aaagggcaaa ctgaaattgc agtcgcatct cgatcgtgaa gagtacgttg
    15481 ccagagttct ggatcgcgag gcgaaaagta cgccacctga ggcagcgaaa gccatgactg
    15541 tggcgatacg cactttttta cagcaaaacg ccaatcggga aggcgattgc ctgaccatcc
    15601 ccgacagcag cgccacgcag cgtgtttctg cttcgccagc taccacgggg gcgcgaacga
    15661 tgaccgtctg gacgcaggat ctcatctatg cgggcgatcc ggttcattat cacggcagtc
    15721 gcgctaccga agggacgctt tcctggcggc aagccatggc ccaggcgggg aagggagagc
    15781 gttacgacca gatactcgcg tttgcttatc ccgacaacag ccttagccgc tggggagcgc
    15841 cacgcacaac ctgtcagtta ttacccaaag cgaaagcctg gctggcgaaa aaaatgccgc
    15901 agtggcggcg tatattacaa ggcgagacgg ggtacaacga accagacgtg tttgcggtct
    15961 gtcgtttagt ctcaggtttc ccctataccg atcggcagca gaaacggctg tttattcgta
    16021 acttctttac gcttcaggat cggctggatt taacccatga gtatctgcac ctggcctttg
    16081 atggttatcc caccgggctt gatgagaact atatcgaaac gctgacccga caattattaa
    16141 tggactaatg ctatgcgaaa gatttttctt ccgttgttac tggtggcgct ttcgcctgtc
    16201 gctcacagtg aaggtgtgca ggaagtcgag attgacgcac cgctctctgg ctggcatccg
    16261 gcagagggcg aagatgccag tttttcacaa tcgatcaatt acccggcgtc gtccgtcaac
    16321 atggcagatg atcaaaatat ctcagcgcag atccgcggca aaataaagaa ttatgctgcg
    16381 gcgggtaaag ttcagcaggg ccggctggtg gtcaacggtg ccagtatgcc gcagagaatt
    16441 gaatccgatg gttcatttgc acgcccttat attttcactg aaggcagcaa cagcgtgcag
    16501 gtcatcagcc cggatgggca aagccgacaa aaaatgcagt tttactcaac gccgggtaca
    16561 ggaacgattc gtgcacgttt acggctggtt ctctcgtggg atacggacaa taccgacctc
    16621 gatcttcacg ttgtcacgcc cgatggcgaa cacgcctggt acggtaacac cgtgctgaaa
    16681 aacagtggtg cattggatat ggatgtcacg acggggtacg gacccgagat tttcgccatg
    16741 ccagcaccag ttcacgggcg ctatcaggtg tatatcaact actatggtgg gcgcagcgaa
    16801 acggaattaa ccaccgccca actgacgctg atcaccgatg aaggatcggt caatgagaaa
    16861 caggaaacat ttattgtacc gatgcgtaat gctggcgagc tgacgctggt gaaaagtttt
    16921 gactggtgat ccatcccttc acacaacctg gctctttctt ttttcagata agactgctgg
    16981 gcgatctgcc gacgccagga tataaccatt aacaagcgtc ggcagattgg tcatttctta
    17041 tttttcgtac tgagaactgt ccatttactc cggctttcct ggttaaggca atggtttcta
    17101 agcgatacgc attattgcaa tggtgacaga acattgcggt aacacgcttt taccgctacc
    17161 ttaaccacac tccatcggtc acctgaggcg gagcttcgcc cctttgaaat accttgcttc
    17221 ttttcgtaca accctgaaag cctcgcgcta catgttcaga gcattggcgt tagtgctctg
    17281 gctgttgatt gctttttcat ccgtttttta catcgttaat gcgttacatc agcgagaatc
    17341 ggaaattcgt caggaattta atctgagttc cgatcaggct cagcgcttta ttcaacgcac
    17401 ctctgatgtg atgaaagagc tgaagtacat cgccgaaaat cgcttatcgg cagaaaacgg
    17461 tgtgctttcc ccgcgtggac gagaaacgca ggcggatgtg cctgcgtttg aaccgctgtt
    17521 tgccgactcc gattgttcag caatgagtaa cacctggcga ggttctctgg agtcattggc
    17581 gtggtttatg cgctactggc gcgataattt ttctgcggct tacgatctca accgggtatt
    17641 tttaatcggc agcgataacc tctgcatggc caatttcggt ctgcgtgata tgccagtgga
    17701 acgcgatacc gcgttgaaag ctttgcatga acgcatcaat aaatatcgaa atgcaccaca
    17761 agatgatagc ggcagtaacc tctactggat cagcgaaggt ccgcgccctg gcgtcgggta
    17821 tttttacgcg ttgacgccag tttatctggc gaaccggttg caggcgcttt tgggtgtcga
    17881 gcagaccatc cggatggaga actttttctt accgggtacg ttgccgatgg gggttaccat
    17941 tcttgatgaa aatggtcata ccctgatttc gcttaccggg ccagaaagca aaattaaggc
    18001 cgatcctcgc tggatgcagg aacgctcctg gtttggctat acggaagggt tccgggagct
    18061 ggtgctgaag aaaaatctgc caccctcatc gctcagcatc gtgtattcgg tgccggttga
    18121 taaggtgctg gaacgcattc gcatgttgat ccttaacgca attttgctga atgtgcttgc
    18181 cggagctgca ttgtttactc tcgcgcggat gtacgagcga cgtattttca ttccggcgga
    18241 aagcgacgcc ctgcgactgg aagaacatga gcagttcaat cgcaagattg tcgcctccgc
    18301 gccagtgggt atctgcattt tgcgtaccgc tgatggcgtc aatattttaa gtaacgaact
    18361 ggcgcatacc tatctcaata tgcttacgca tgaggaccgc caacgactga cacaaattat
    18421 ctgtgggcag caggtcaatt ttgttgatgt cctgaccagc aacaatacca atctgcaaat
    18481 cagcttcgtc cattcgcgct atcgtaatga aaacgtggcc atttgtgtgc tggtggatgt
    18541 ttcttcgcgc gtgaagatgg aagagtcgtt gcaggagatg gcacaagcag cggaacaggc
    18601 gagccagtca aaatcgatgt tccttgccac cgtcagtcat gagctgcgaa cgccgctgta
    18661 tggcattatc ggtaacctgg atctgttgca aaccaaagag ttaccgaaag gcgtcgatcg
    18721 tctggtgacg gcaatgaaca actcttccag cctgttgttg aaaattatca gcgatattct
    18781 cgatttctcg aagattgaat cggaacagtt gatgatcgaa ccgcgtgagt tttcaccgcg
    18841 tgaagtgatg aaccacatca ccgccaacta tttaccgctg gtggtacgca agcagttagg
    18901 cttgtactgc tttattgaac cggatgtgcc agtggcctta aatggcgacc cgatgcgttt
    18961 acagcaggtc atctccaacc tgttgagtaa cgccataaaa ttcaccgata ccggctgtat
    19021 agttttgcat gtccgcgctg atggcgatta tctctctatc cgtgttcgcg ataccggcgt
    19081 ggggattccg gcgaaagaag tggtgcgctt gtttgatccc ttcttccagg tcggaacggg
    19141 cgtacagcga aacttccagg ggaccggtct gggtctggcg atttgtgaaa aactgatcag
    19201 catgatggac ggcgatatct cggtagattc agaaccggga atgggcagcc agtttaccgt
    19261 gcgtattccg ttgtacggcg ctcagtaccc gcagaaaaaa ggcgtggaag ggttgagtgg
    19321 taaacgctgc tggctggcgg tccgcaatgc gtcgctctgt cagttcctgg aaaccagttt
    19381 gcagcgcagc ggcatcgtcg taacaacata cgaagggcag gaaccgactc ccgaagatgt
    19441 gttaatcact gacgaggtag tgagtaaaaa atggcagggc agagcggtag tgaccttctg
    19501 tcgtcgacat attggtattc cgctggagaa agcgccaggg gagtgggtac acagtgtggc
    19561 tgctccgcat gagctaccgg cattgttggc gcgtatttat ttgatcgaga tggagagcga
    19621 cgatcctgct aacgctctgc cgtcgacgga caaagcggtc agcgataatg acgatatgat
    19681 gattctggtc gtggatgatc atccgattaa ccggcgtttg ctggcagatc agttgggatc
    19741 gttgggctat caatgtaaaa ccgcgaatga tggcgtcgat gcgcttaatg tacttagcaa
    19801 gaatcatatt gatatcgtgc ttagcgacgt caacatgcca aatatggatg gttaccgctt
    19861 gacacaacgc attcgtcagt tgggactgac gttgccggta atcggagtaa cggctaatgc
    19921 gttggctgaa gagaagcagc ggtgtctgga gtccggtatg gacagctgcc tgtcgaagcc
    19981 agtaacgctg gatgttataa aacagacgct gacggtatat gccgagaggg tcaggaaatc
    20041 gcgggaatcg taggacggat aaggcgttta cgccgcatct ggcattcagt gcaaatgcca
    20101 gatgcgacgc tgacgcgtct tatctggcct actttaatgt aatctgttga aataatggga
    20161 atcgtaggac ggataaggcg tttacgccgc atccggcaat cggtgcaaat gccagataag
    20221 acactaacgc gtcttatctg gcctacagat gattagtctt tatctgccgg acttaacgtt
    20281 actgaagaga gataattcag cagggcgata tcgttctcga cacccagctt catcatcgca
    20341 gatttcttct ggctactgat ggttttaata ctgcggttca gctttttagc gatctcggtc
    20401 accaggaagc cttccgcaaa caggcgcaga acttcactct cttttggcga gagacgtttg
    20461 tcaccgtaac ctccagcact gattttttcc aacaggcgag aaacgctttc cggggtaaat
    20521 ttctttcctt tttgcagcgc tgcgagggct ttcggcagat cggtcggtgc accttgtttc
    20581 agcacgatcc cttcgatatc cagatccaat accgcactaa gaatcgccgg gttgttgttc
    20641 atggtcagaa caataatcga caggcttggg aaatggcgct tgatgtactt aattaaggta
    20701 atgccatcgc cgtacttatc gccaggcatg gagagatcgg taatcaacac atgcgcatcc
    20761 agtttcggca ggttgttgat cagtgctgta gagtcttcaa attcgccgac aacattcacc
    20821 cactcaattt gctcaagtga tttgcgaata ccgaacaaga ctatcggatg gtcatcggca
    20881 ataattacgt tcatattgtt catgtaatag gctaccttgc tacagcaagc tcttgacata
    20941 actgtcaatg tcgctgatgt atttttctat tcctggaaca tccttctcac gaatcagatg
    21001 ttccagcgtt tcacataact gcttgccggg taccagattt agcatggcaa atacgccttt
    21061 aagacgatga gccgtttggg ctaacgcagc aaagtcactg gttgctgctt cagtatacag
    21121 cctcttaaca tcatccggta ctgtgtctac aaagagcgca taatagccgc tggcatggag
    21181 ttgcgcattt tcatctccgc ccagaggcga ttctgtcacc tcttcctgcg ccagttgcac
    21241 ttcaattaat tgtaagaccg cttcctgcat agcgttgctc atattgaagt tgacgcacaa
    21301 ttgaccaggc ccaatttccc gtacgccaga ctcatcatcg cttaaaagca agccagaggc
    21361 agtaagatta gacggattat ccgttaaaaa gatatcataa tcttgactaa ttaatctttc
    21421 atcgggtgtg atacaggttg caccccaatt ttctaactgg cgagtgacaa tattccgaat
    21481 ttctgccgaa gtaacatcca ccattacgca gacatcatcc agtaaacgct cttcttcctc
    21541 ttcaacttcc gggtcagctg cgagcatttt gatatgcaca gagtagcgtg taccaagccc
    21601 atcccgcgtt ttgatgttta aatgaccgcc cagtttacgt gccagttgat cgctcagcca
    21661 gaatgccagc gggtccgcct tgccatagcg atcgttttgg gtctggttga taaacgggaa
    21721 gtgcaaatta tccatttcat gaatgcttac gccttctccg gtgtccagaa tgcggaacgt
    21781 caggcggtct tcggaggact catcctgatc aacctcaagg gtgatttttc ccaattgcgt
    21841 tgaggtcacg gcatattgca tcagtagcag caaaatacgt cgtaatgcat cgcgatcgcc
    21901 gcggcgcata tcgtgtgctt tcagatgatt gttaatcagc agttgcagac ctttacgctt
    21961 gatggcaggc aacactgaag gcacaacttc atcaattaaa tcctgcacgg agaacagcac
    22021 cgtctcactt ttccagctat cgtccgcaag catgttcgct aactgtattt catcgaccaa
    22081 tcgcaccagc acatctgcct gattcgccag ttgtttgctt tccggggcgt tgagtttagc
    22141 cgcgctctcc gccagggact gtgcgggttc tttcagcgca tcgccaatgt ttttcataaa
    22201 ggtcatccgc ccctgctggt ttttctcata cagacgctgc gcctgcttga gtttcttgtt
    22261 taccagcact tcgcgatcct gatcgcgaat aatgaaaatt tgtgtgcgcg gcgcaacctg
    22321 gctgcggaac atgcggatct catacagctc gttattgatc gtcgcctgaa taatcccctg
    22381 atgctgttcc gccatggtgg tgatgttttg cagattcaaa tgcggcagca aatgatcggc
    22441 aattttgtta cttatgacag tgcggttcga ttcctgatcg tgaaccagca ggccgagcgg
    22501 cagcagtgag actatctctt cattgattgc ccgtaaaatg cgcaattcgt tattgaccgc
    22561 cgtgttgggt agactttctg tactgcgact ggagaaatgg cggaatgtgg tatagccaaa
    22621 taacgccagc gccagcaaac cgatgttcag cagcagtggc agcagaatgt tttgcaacgt
    22681 atccagcaat aaggtgccat aaggaacctg ccagaccagg cgcatatcgg tagagttgag
    22741 tgccgaggag atttcaatct tcgtactgtt aaagtggata ctgacgctat ccgttccttc
    22801 tttctcatta tcattgtttc ccgttgccgt cgcgtctggc tcaaggcgga aactgtccag
    22861 cggcataccc ggtgggatca aatcattaat cggcagatca aaagccacga ccgttgccag
    22921 atgtcccggc tggttgaacg tggtacgcaa ggtaaagtaa tgaccgttct gccaggccag
    22981 gcggcgcatg ttagaaaaac tttcgcgttc atcgagggcg ttggcctgtt gcaacatctc
    23041 tgcacgacgt gaatcaacaa tgtcactgac ggtcgattct ttaaatccgg aggtgagatc
    23101 tttgaggggc agggttgaga tcagcaccag actgttatcc tgaccattca ggtaatacat
    23161 tgaccacggt acattttctg cgccccacaa tgtgtccaga taggtggaca tccgttgagt
    23221 catctcaagc gttgagttgt cgtgagagcc aaagattaac gcttccgttt tgcggcgcgg
    23281 tttttccaga tagtagacat cctgtttcag gcgcgtctct tgtaaacctt cgccggagga
    23341 gggggaggtc gtcgcggcaa tgttgtcgta gatctgccag gtcacgtaac gccaggtatc
    23401 gacgcgcttt tggatagcgt gggtaatgtc gacaatttgg taacttttat ctttcagcca
    23461 ggcgttaacg gcgctttgta ccattacacc catcgtcacc agtaacacaa tgatcaacag
    23521 taaaaagaag cgggtaatgc tccccggtag gagtgaaaag cgggtcgtgg ccgttgtctc
    23581 tttctgacgc attcgtgttt atgacctgtt aaaacttcgc gaagtgatag ggcagtataa
    23641 agggtacagt gtgaaattcc agactcttac ctgattgatg attctctatt ctcccgtgat
    23701 atttatcatc ctgcattggg ggaaatagat gtacaagcgc cattttgctg tacaaataac
    23761 ctacaaaaag cccaacgaaa ataattgcaa ataaacaaag attgctggaa attatgctga
    23821 tgttaattat ttgtgaaata gttaacaagc gttatagttt ttctgtggta gcacagaata
    23881 atgaaaagtg tgtaaagaag ggtaaaaaaa accggatgcg aggcatccgg ttgaaatagg
    23941 ggtaaacaga cattcagaaa tgaatgacgg taataaataa agttaatgat gata
//
LOCUS       DAZKIF010000073        23870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZKIF010000073 DAZKIF010000000
VERSION     DAZKIF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.073"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..325
                     /locus_tag="ACHOTW_002809"
     CDS             <1..325
                     /locus_tag="ACHOTW_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9844883.1"
                     /translation="ITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWS
                     LTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQP
                     VSLAG"
     gene            complement(368..1519)
                     /locus_tag="ACHOTW_002810"
     CDS             complement(368..1519)
                     /locus_tag="ACHOTW_002810"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP9844884.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1533..3128)
                     /locus_tag="ACHOTW_002811"
     CDS             complement(1533..3128)
                     /locus_tag="ACHOTW_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP9844885.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3273..4031)
                     /gene="aroD"
                     /locus_tag="ACHOTW_002812"
     CDS             complement(3273..4031)
                     /gene="aroD"
                     /locus_tag="ACHOTW_002812"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9844886.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4062..4928)
                     /gene="ydiB"
                     /locus_tag="ACHOTW_002813"
     CDS             complement(4062..4928)
                     /gene="ydiB"
                     /locus_tag="ACHOTW_002813"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP9844887.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4940..6205)
                     /locus_tag="ACHOTW_002814"
     CDS             complement(4940..6205)
                     /locus_tag="ACHOTW_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9844888.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPKNDVRFGERKFSTRLNTIKHRG"
     gene            complement(6432..7646)
                     /locus_tag="ACHOTW_002815"
     CDS             complement(6432..7646)
                     /locus_tag="ACHOTW_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9844889.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7746..8102)
                     /locus_tag="ACHOTW_002816"
     CDS             complement(7746..8102)
                     /locus_tag="ACHOTW_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP9844890.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8238..8345)
                     /gene="rprA"
                     /locus_tag="ACHOTW_002817"
     ncRNA           complement(8238..8345)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHOTW_002817"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8531..9643)
                     /gene="ydiK"
                     /locus_tag="ACHOTW_002818"
     CDS             complement(8531..9643)
                     /gene="ydiK"
                     /locus_tag="ACHOTW_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP9844891.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
     gene            10032..13088
                     /locus_tag="ACHOTW_002819"
     CDS             10032..13088
                     /locus_tag="ACHOTW_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP9844892.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13085..13495
                     /gene="menI"
                     /locus_tag="ACHOTW_002820"
     CDS             13085..13495
                     /gene="menI"
                     /locus_tag="ACHOTW_002820"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP9844893.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            13595..13783
                     /locus_tag="ACHOTW_002821"
     CDS             13595..13783
                     /locus_tag="ACHOTW_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP9844894.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14332..14700
                     /gene="sufA"
                     /locus_tag="ACHOTW_002822"
     CDS             14332..14700
                     /gene="sufA"
                     /locus_tag="ACHOTW_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP9844895.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14709..16196
                     /gene="sufB"
                     /locus_tag="ACHOTW_002823"
     CDS             14709..16196
                     /gene="sufB"
                     /locus_tag="ACHOTW_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9844896.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVVSDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16206..16952
                     /gene="sufC"
                     /locus_tag="ACHOTW_002824"
     CDS             16206..16952
                     /gene="sufC"
                     /locus_tag="ACHOTW_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9844897.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16927..18198
                     /gene="sufD"
                     /locus_tag="ACHOTW_002825"
     CDS             16927..18198
                     /gene="sufD"
                     /locus_tag="ACHOTW_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9844898.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            18195..19415
                     /gene="sufS"
                     /locus_tag="ACHOTW_002826"
     CDS             18195..19415
                     /gene="sufS"
                     /locus_tag="ACHOTW_002826"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP9844899.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19428..19844
                     /gene="sufE"
                     /locus_tag="ACHOTW_002827"
     CDS             19428..19844
                     /gene="sufE"
                     /locus_tag="ACHOTW_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP9844900.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19993..20997
                     /gene="ldtE"
                     /locus_tag="ACHOTW_002828"
     CDS             19993..20997
                     /gene="ldtE"
                     /locus_tag="ACHOTW_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP9844901.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21060..21296)
                     /gene="lpp"
                     /locus_tag="ACHOTW_002829"
     CDS             complement(21060..21296)
                     /gene="lpp"
                     /locus_tag="ACHOTW_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP9844902.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21607..23019)
                     /gene="pykF"
                     /locus_tag="ACHOTW_002830"
     CDS             complement(21607..23019)
                     /gene="pykF"
                     /locus_tag="ACHOTW_002830"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP9844903.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(23027..23221)
                     /locus_tag="ACHOTW_002831"
     CDS             complement(23027..23221)
                     /locus_tag="ACHOTW_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844904.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            23576..23785
                     /gene="fumD"
                     /locus_tag="ACHOTW_002832"
     CDS             23576..23785
                     /gene="fumD"
                     /locus_tag="ACHOTW_002832"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP9844905.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         6225 a         5758 c         5997 g         5890 t
ORIGIN      
        1 aatcacttta gaacagttat cgaaaaaatt tcgtgccagc gtcagttata tttgccatga
       61 atttaccaaa gagtatcgta tttcccctat taactatgtt attcaacggc gtatgacgga
      121 agcaaaatgg tcactcacta atactgaatt atcacaggca gagatctcct ggcgtgtggg
      181 ttatgaaaat gtcgatcact ttgccaaact gtttttgcgc catgtcggct gttcgcccag
      241 cgattaccgc aggcaattta aaaactgttt tgcggaacaa gaaatcctat ctgaatttcc
      301 tcaaccggta agtcttgccg gataaatctg gcggcgcagt ttgttaagaa gctgcgccgc
      361 ctgcagatta tttgttctga tagtctttca ggatctgccg acctgctacg taaatcataa
      421 tttcgtctgt accgccgcca atacgttcac aacggacatc acgccagaag cgggagacgc
      481 gcgcctcatc ggtatagccc agaccgccca tgatttgaat cgcatcatca atgacttcca
      541 ttgcggtacg tgcacagtac agttttgcca gcgccgcgct ggtgcgcagt gattgatgct
      601 gatcggcttg ccatgccact ttcagcacca tgttgcgcat gttgtcaatc ttaatcgcca
      661 tcagcgccag tttttcctgg atcatctggt tatgaccaat gggtttacca aaagcgatac
      721 gctggttggc atagcgagcg gcatcttcaa aggcacactc ggcaaagccg gtgctgcgcg
      781 cggcgttgat caggcgctcc atctcaaagt tgtacatcac attgaggaaa cccattcctt
      841 cttcgcccac catgtcgctc tcttcaactt ccacgttgtc gagatagact tcgcaggtgc
      901 tgagcatatg ccagccgatt ttatgcagcg ggttaatctt aatgccgggc ttactggagt
      961 cgacccacca cagggtgaag gcttttttgg gatctttcgg ttgcggatcg cgcgccagca
     1021 ccagcatata cgggtactct ttcgcaccgg taataaaggt tttctgtccg ttgatgtaaa
     1081 ccttgccgtt tttacgcgtg taagtggtag tggcactgtt gttatctgag ccagcacctg
     1141 gttccgtcaa cgccagggca taggcgggat cacccgtttc cagggtgctt tctgccgttt
     1201 tacgtagctg ctctgcagaa ccgaaacggc gcatactgtg aatacattga ccgttggtaa
     1261 ttaaaaatgc cggagcaccg cattttgaca cttccatcag cgccagcatt tgggtgacgt
     1321 aatcagcagg gataccacca aattcttccg gcacgccaag catggaaata ccgttatccg
     1381 ccagtgcccg cataaactca cgcggatatg tcccgttttg atcgcaggtg cggaaatact
     1441 cttccggaaa gttagtcgta atcagttcgc gaatactggc cagcagcagt tcttgttctt
     1501 cagttaaaga aaaatccatc gtgatactcc tgttaatgag ccgcttcagg caggacaaaa
     1561 cccatcgccg catcgataaa taatctttcg tccatcagtt tgagttctgg cgaaatcact
     1621 ggggtgaaat ccattttgtc gagaatatct ttttgtaaat cgacgccagg ggcgatttcg
     1681 attaaatgca gtccgtcttc tttcagcgtg aatacggcgc gctcagtgat ataacgaaca
     1741 tccagccctc gctcgagagc gatttttccg ctaaaagtaa tttccggtag ttcccgaata
     1801 aatttcttca cccgtccttc ctggacgata ttcaatttgc catcggcaat ttctgttttt
     1861 aaactgcccg cagttaacgt gccgcagaaa ataattttct tcgaggtggc actgatatca
     1921 ataaatccac cggtacccat gattttgcca ttgaatttat gcacgccgac gttaccgtgc
     1981 tggtcgactt cagcaaaact caaataacaa acatccagac caccaccgtg ataaaaatca
     2041 aactgggacg tcatatccag aatggcacgg gtattcacgt tcgcgccaaa ggcgataccc
     2101 tgtgaagtaa ttccaccaat cggacctgtt tctaccgtca gaataaagtc atcagcacaa
     2161 ccttcttccc gggcgaccag gccaatgccg tcagcaatac cgacgccgac attccccacc
     2221 gcgcctttac gcatttcgaa taatgcacgc cgtgcaacta atttacgttg gtttaggggc
     2281 aggctaagtt tggtgctgtc atcaagggtg aagtcaccag aaataaagcg gttaaccggc
     2341 gcaccgccat atagttggct ttgatccgga tcgaccacca caatatccac cagataaccc
     2401 ggaatacgga cagatttagg atgcagtgtg gctttcttaa ccattttctg cacctgcatc
     2461 atcacaatac cgccgttatt gtgcaccgcc tgggcaataa ccaatgcgtc gagatacatc
     2521 acctcatctt caaaagtggc gtagccttca ctgtcgcagg tggtagcgcg aatgaaagca
     2581 atatctggcg caattgcttt gtaatagaga tattctttgt tatcaaactc gaccagttta
     2641 atcaggtctt ctttagtgac ttcattcagt ttgccgcctt gctggcgtgg atcgacaaat
     2701 gtcccgatgc caatatcact aataatacca ggctggtggg ctgcggcggc gcgtaaggtt
     2761 tgtgtaagta caccttgtgg atagttataa gcaataattt tattttgttc tgcgagatca
     2821 gaaatacgcg gcgattgtcc ccagtgaccg cataatgccc atttcaccag accttcttgc
     2881 gccagcggac taataccgcg gtcggcgcga tcgccaagcc ctgttggact aataatcgat
     2941 aaattacgtg gcgtttgagt ctgtttatat ttatcagcaa gagcagtaat taacgtggtg
     3001 gcttccagaa taccgccgcc agcgcctaac acacaaagtg ttgcttcgtc gggaatataa
     3061 ttcaccgctt cctgtgccga caggaccggc actcgtccat taatacgagg tggttttaca
     3121 ggtttcattg ttaattcctg attcaggtgt gattttccct acgccagatg gataattagc
     3181 gcacagagac tcacgaaata tttttattaa atacggttga atatattttt ttagttcggc
     3241 gggggaggat attcccgccg aaatattatt gcttatgcct gatgtaaaat agttaatact
     3301 gtgcgcaaat catttaccga gatttgccct ggcgcagagg ctttttttac cgcaccaaaa
     3361 gttgccgccg agccaaatac ttcaccagcc agacgagaaa ttacgccagt ttttgccatt
     3421 gacatcgtga tgattggacg atcggcatac tgctcctgca tctccagggt cgcggcaagc
     3481 aacgccagca catcgctggt actttgcggc atcagcgcaa tcttaggaat atcggcgtcg
     3541 aaggactgca ttttgcgcag acgggcaatg atttcttcgg cttccggcgt tttatggaag
     3601 tcatggttgg acatgacaac tttcacatca tgcgcgtggg cgtaggcgac ggtttcttta
     3661 acctgatcat cgccggtaaa taactccaga tcgatcatat caaccaggcc gctgtcgatg
     3721 gctgcacgat tgagagcaat ataagcctcg gtggaaatcg cctgctcgcc gccttctttg
     3781 gcactgcgga aggtaaacag cagcggtttt tctggcatgg tttcacggag aatttttgcc
     3841 gccgccatga cagactccac attggagagg tcggcaaagt ggtccacacg ccattccaga
     3901 atatcaaagt ccgcttcacg ataggcgaga gcttcggatt tcactcgggc gatatctttc
     3961 gccatcagcg agacgatgat tttaggtgcg cccgtaccaa tgacgagatc ttttacagtt
     4021 acggttttca ttttttaccc tttctgcacg cggtcagcct gtcaggcacc gaaccccatg
     4081 acctgtttaa catattccag agggaaatct ttgccagtcc ataacgtgaa ctgttcagcc
     4141 ccttgccaca acaacatgcc gtatccatca atcgttttgc aaccagcttg ttgcgcctgc
     4201 tgcaataact tcgtcatatg cgggttatac acgcattcag tgaccagcag tcccggatgt
     4261 aacagactga tatcattaac caatgattta ttctcaaggg gtttcatacc cacttttgtg
     4321 ccattggtta aaatgtcggc ggaagccagg gcttcagcaa aggcttgctg atcggcgaga
     4381 tcggtaaccg tgacgacaca atcggtgttt tcgttaaccc gctgcgcgaa ggcgagggct
     4441 ttatcgaaga acttatcccg acggttaaag agtttaattt cttttaaacc ttcaattgcc
     4501 ccctgcgcgc caatcgccgt tgaggcacct ccggccccta acagcaccat tgttttgcct
     4561 ttgatatcaa aaccgctctc tttaatggcg cgaatatggc ccgtgccgtc ggtgttatag
     4621 ccgcgcaggt agccatcatc attaacgatg gtgttgatgg cacccaccag tttggctgct
     4681 ggcgttaatt catcaacata ttcacacgcc agttgtttgt tcggcattga tacaccagtt
     4741 ccgcgcattt ttagagcttt taatccttca attgctccgg gaaagctatc gttatccact
     4801 tcgaaggcca tataggtaaa tggcaatccc gctttttcta aggctttatt ctgcatttca
     4861 ggcgataaac tgtggcggat aggataagcc atcaacccaa tcaattcgta ttttgcggta
     4921 acatccataa ttaactcctt taacctctat gcttaattgt gtttaaccgg gtgctaaatt
     4981 tgcgctcgcc aaaccgcaca tcattcttag gaataataaa aacgcggtaa tagcggataa
     5041 aaacaataat tgcggtaatc agggccagca gcgcgaaagt aaaatcgaga acaatgatat
     5101 attgcaggcc gatgttcgac agataaccgg taatcagcgg aataacaaag ttagccagtc
     5161 cacccatcat catataaata ctggtgactt tggctttgct ttttgggaaa aactctgaca
     5221 tgaccgaaac accgagctgt aaaatgccgc cagctgctga gaaaccgata acaaaggcgc
     5281 cggcattaca caccagtgga gaagggtaca ggtagataat ggctgctgtt attgttgcca
     5341 gtgcagagtt aaatacatta gcccagatgg gccggaccat ttttttcagt agtgcggcaa
     5401 aaataaagac acagaccagc gagcccatac tgtaataaga gatggttttt aatgcctcag
     5461 cttctgacat accagcaaaa gccatcgcat atttgggcat ccacaccaca atcacataaa
     5521 aggtcgagaa tgcggctaca ccgaacagta ccgatgaaac accttccagc cagactaacg
     5581 gtttgctgtt catttgcggt aattcattag ctacgctggc gtccaccaac tggctgggga
     5641 atttgctttt caacagcatc agcgtgatca gtacaaacag aatacccgga ataatcagcc
     5701 catagccgta ccagatatta ttgagcaaca tatagctcac cagcattggg tagaacattt
     5761 gcccaaatga caccatcgct ttaaccagta tgaccgccga accagaggct ttcggaaagc
     5821 attccatgag cgcggggtag ccacccgtat ccagcgctga gttagcgata cctacgcaca
     5881 ctgccagacc gtaggcgaga gttaaattcg ggcaagcggg aataccaaag aagaatagca
     5941 gatacattat tactgccatt aatatcaccg cccgacgacc aaacttatcg gagatcacac
     6001 cgaagaataa aatactgatc aatcgcccca aaccgatacc ggaaattaag taggcaatgc
     6061 ccgcgttgtc agtggaaaac ttttccgcca gagatgacat attttgggca agcgtaataa
     6121 cactaatgcc gtgcaggaag tagctgaagt aaatacaaag aacagccagg ataaatggcg
     6181 tgctgaaagc cttattttga gacattttca cagctccttg ctgaaagata atttcgttac
     6241 cgtacgtatt gttctccgac ggtttacata attactatac tgaagatgat tttccctggg
     6301 tacatggcat aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc
     6361 gattttcctt agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa
     6421 agaatattgc attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt
     6481 ttgcggctgc gtaagccgat aaacagtgcc agtaaaaaac cgatggcagc gatggcggta
     6541 tcgaaccaca taatatcggc aatacttctt tgggacagat gagccgtaat caacggaata
     6601 gtaaaggtcg caatactgcc cgcactgtaa tagatccctg tagctttacc tttagcgtaa
     6661 gggaaacgtt cagccattaa cgtcaggcca atttgcacaa cacctccagc agaagtaaaa
     6721 ccaatgacaa aagaaaatat tatcaccaca taaaatgtgg gatgcaggca gacggtaaac
     6781 agagcaataa atgagataaa ggtgtacagc atcagtaatg ttgttgggcg aacggtatta
     6841 cgaatgagtg gagcggtaat aaatacacaa agcagcgaac ccacggtgta gatactgagt
     6901 agtttgatcg acatagtgta tgacatgcct gcaacaaatt gtccgtactg tgccagccac
     6961 tggctaacca gataaaacgt tgccattgag atatagccat ataaggtata actggctaaa
     7021 tcgataattg aacagcgatg ttccgtagag ctggtggttt tctttatgac aggtaagcga
     7081 cgacccggat ggggtgggaa cgtacaacgg tataaaaaca gagcgttaat aaacataatg
     7141 cctgcagcaa tcataaagga ccaaccgaac cacagttcag cccacactaa caggctaatg
     7201 attagcggta ataaaaattg tccgctggaa acaaatgctt taattaaaat attggctgtc
     7261 ccaggtgagc gtggaaaagc ttccatcaaa ctgggataag tgcctgcatc gagaaaactg
     7321 tttgccattc ccgccagaaa gccaaaaaca taagcgataa tgatgttatt ggtctgcagg
     7381 atgccaaaaa agaaggccat atagcagcac atcccgagca tgataaaagg gcggcgacca
     7441 aagcgatcgg ataataatcc tgcaaaaagc aagacactta atcgaccaat gcccagcgat
     7501 gagataacta tcgagacacc cgcggcatta gactgccaaa gtgtctccag cgaggccata
     7561 ttcaggctca tcaaaatgac gcccatacca tgcaccaggt aattaaaata caacccaagt
     7621 gcggtagggt aatagggatt tttcataggt tctacttagc aaaggtgctt taaaaagtgt
     7681 agcgatgttg tgtcagtatc cgttcattat tgttgtaaca acgacaattc ctttttgtgg
     7741 agaaattaac aaagccgctc cagatcgtgt gcatataatt cagcggctac cgattgatag
     7801 atcttccagt caataaaatt gccatcaggg tagacttgtt gaaacgcggt taagtcgggg
     7861 aaaaaacgca tggtgttgtt gccgatgcgg ttatttattt tctctatgat cgcatgaaga
     7921 tgtttttttc tttgctgacg catagccaac agttgctcga ctacacaatc aggaatggca
     7981 cacctgccat tttcccattg acgccagctt tcactgtcac cggtctgggc aatccaggtc
     8041 gcgcattcat caatagtcat ggcaaaaata tggcggagtg cctggagttc ataagcgttc
     8101 atcgtgtaat ggctgcatga aataaacaat aaaatataaa taacattatc agcctgctga
     8161 cggcttgaag agagtcacag tatcttgtgc aacattattg gaaagagtga ggggcgaggt
     8221 agcgaagcgg aaaaatgtta aaaaaaagcc catcgtggga gatgggcaaa gactacacac
     8281 agcaattcgt tgtttcactc aggggatttc catgcttata aatcaatatg ttgatttata
     8341 accgtgagct aatagtaggc atacggactt tttgcgtcga tcagattcgt ctcattagtt
     8401 taaaaactgt aaagaatttg cgacaggtac taccagctga atggcggtaa gcaggaaaat
     8461 atggggaaga gcaagcaaat atcaattagc ggtggttaac aatcaggcgg cagagccgcc
     8521 tgatgagaaa ttacttattc ggtttctgga tttcgccaag ttcttcgaga atttcttccg
     8581 gttgatccgt cggcggtggg acctcttcca cccacgcggc aaacagacgc caggaaacgg
     8641 ctaacagaac cggaccaata aacagaccga tcatcccgaa agcaatcaaa ccaccaataa
     8701 cgccagagag gatcaggatc agcggtaaat cggcacccat gcgaattaac attgggcgga
     8761 tgacgttatc cagcgtgcca accacaccgc tccacactaa caataccgtt ccccaggtgg
     8821 tatcgccagt ccagtagagc cagataatcg ccggaatcag taccggcaac gggccaagct
     8881 ggacaaggca ggaaaggatc attaacaccg ttagcaaagt tgcataaggt acgccggata
     8941 cggcgaggcc gataccgcca agcactgcct gtactaacgc cgtcaccacc acacccagcg
     9001 ccaccgcgcg gatagcctgt gccgccagca gtacggcggc atctccgcga acgcctgcca
     9061 gacgggtcgc aaaatggcga atgccttgtg ccacctgttc accgcgccag tacagcaggg
     9121 cactgaagag aagcatcagc gcacaatgca ccataaagcg cccgatatgc gccgcttgcc
     9181 caacgaacca ggtagtggtg gtgccaatat aagggcggac tttcgccatg atcgccgtcc
     9241 cccccatatc cagcaagttg tgccagcctg catacagctt cgcgccaatc accggaatgg
     9301 tattaagcca cgccagatcg ggtaacgtca tgtcaccgct ggaaatggct ttaattagcg
     9361 ggccgctgcc gtcgacgata ctgttaacca gcaaagcaat agggatgata aacaccatca
     9421 ctaataacag cgtcatcacc agaacggcga gagagcggcg gccaaacatg atcttttgca
     9481 aacgtaacaa taccggccag gtggcgataa ccaccgtacc ggcccatgca aagccgagaa
     9541 taaagggttg aacaatccac agacatgcca caatcatgat ggctaaaaac agcaccgaaa
     9601 gcagaatttg tgcgacatcc ctgggctgac gaacatttac cataactact tttcaccttt
     9661 tttgtgcgtc aaatcgtcga cgcgacgtga acacgcgaaa ctcacctgca taatgatgag
     9721 caatttcagc ggtttttaac aggccgattc tgcccttaat tctgatgggc gtgttagaaa
     9781 aaaatgtggt acaacaactc aggcaacacg caaacgattt actcgtcgta tttcaagttg
     9841 catatgctgc ggctacgttc gttcacccca gttacttact gttgtaagct cctggagttc
     9901 actcgcttgc cgccttcctg caactcgaaa tccatagagt aaataaacat cacctttctg
     9961 tgcgctattg cagccgcctg aaagaacagt gtatcgacct acatcacaga ccgcaggaaa
    10021 gggtcaatat aatgattcca cagatttccc aggcacccgg cgtcgttcaa ctggtgctta
    10081 attttttgca agagctggag caacaaggtt ttaccggcga tacggcgaca agttatgctg
    10141 atcgtctgac aatgtcgacc gacaacagta tttaccaact tctccccgat gcagtggtat
    10201 ttccgcgttc aaccgcagat gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt
    10261 catcgctgat ctttaccccc cgcggcggcg gcaccggcac taacggtcag gcgctcaacc
    10321 agggtattat tgttgatatg tcccgccata tgaaccgcat catcgaaatt aaccctgaag
    10381 agggctgggt gcgcgttgag gccggggtga taaaagacca actcaatcag tacctgaaac
    10441 cgttcggcta cttttttgca ccagaacttt cgaccagcaa ccgggcaacg ctcggcggga
    10501 tgatcaatac cgatgcatcc ggtcagggat cgctggtcta tggcaaaacg tcagatcacg
    10561 tacttggcct acgtgcggta ttgttggggg gcgatattct cgatacgcaa cctttacccg
    10621 tcgagctggc ggaaacgctg ggtaaatcca ataccacaat cgggcgaatt tataacacgg
    10681 tttatcaacg ttgccgtcag caacgccagt taatcatcga caacttcccc aaacttaacc
    10741 gctttcttac cggttacgat ctgcgtcatg tctttaacga tgagatgacc gagttcgatc
    10801 tgacgcgcat tctgacgggt tcagaaggga cgctggcctt tattaccgaa gcgcggctgg
    10861 atattacgcg cttgcctaaa gtgcgccgtc tggtgaatgt caaatatgac tcttttgact
    10921 ctgcgctgcg taacgcgccg tttatggttg aggcgcgggc gctttcggta gagacggtgg
    10981 actcaaaagt gctgaatctg gcgcgggaag atattgtctg gcattccgtc agcgagttga
    11041 ttaccgatgt gcctgacaaa gagatgctcg ggctgaacat tgtggaattt gctggtgatg
    11101 atgaggcgct gattgacgag cgggtaaatg cactctgtgc gcggttggat gagctgatcg
    11161 ccagtcatca agcaggtgtc atcggctggc agatgtgccg tgagctggcg ggggttgaac
    11221 gtatctatgc aatgcgcaaa aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta
    11281 agccaattcc gtttgctgag gatacctgcg taccgccgga acatctggcc gattatattg
    11341 ctgaatttcg cgcgctgctc gacagccacg gcttaagcta cggtatgttc ggtcacgtcg
    11401 acgcaggtgt cttgcatgtc cgtccggcac tggatatgtg cgatccgcaa caagagattt
    11461 tgatgaagca aatctctgat gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt
    11521 ggggcgagca cggcaaaggt tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac
    11581 tttttgcaga actgcgcaaa gtgaaagcgg catttgaccc gcataaccga ctcaacccag
    11641 ggaagatttg cccgccagaa ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc
    11701 gcggtacatt cgatcggcag atccccattg cggtacgcca gcagtggcgc ggtgcgatgg
    11761 agtgtaacgg caacggttta tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga
    11821 tgaagatcac ccagaaccgg attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat
    11881 ggctgcgttt gctggcggat cgcggcgttg atccactcaa actggaacaa gaactgcctg
    11941 aatccggcgt cagcttgcgg acgttaattg cccgcacgcg caatagctgg catgcgaata
    12001 aaggcgaata tgacttctca cacgaagtca aagaggcgat gtcgggctgt ctggcctgta
    12061 aagcttgttc gacccagtgc cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc
    12121 tgcagctcta tcacacccgt tatttacgcc cgctgcgcga ccacctcgtc gctacggtcg
    12181 agagctacgc gccgctgatg gcacgcgcgc cgaaaacctt taacttcttc attaaccagc
    12241 cgctggtgcg caaactctcg gaaaaacata tcggcatggt tgatttgccg ctgctgtcgg
    12301 tcccctcgct acaacaacaa atggtggggc atcgctcggc aaacatgacg ctggaacagc
    12361 ttgaatctct caatgcagag cagaaagcgc gcacagtgtt ggtggtgcag gaccccttta
    12421 ccagctatta cgatgcgcaa gtggtggcgg attttgtccg tctggtcgaa aaattaggtt
    12481 tccagcctgt gttactgcca ttttcgccaa atggcaaagc ccagcatatt aaaggttttc
    12541 ttaatcgttt tgcgaagacg gcgaaaaaga cggcggattt cctcaaccgt atggcgaagc
    12601 tgggtatgcc aatggtgggc gtcgatccgg cgctggtact ttgttatcgc gatgaatata
    12661 aactggcgct gggcgaggag cgtggcgagt ttaacgtctt actggcgaat gaatggctgg
    12721 caagcgcact tgagtcacag ccagtggcta cagtcagcgg tgaatcatgg tatttctttg
    12781 gtcactgtac cgaagttacc gccttgccgg gtgcgccagc acaatgggcc gcgatatttg
    12841 cccgttttgg cgcgaaactg gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt
    12901 acggacatga agcgaaaaac cataaaaatt cgctcgggat ctatgagtta tcctggcatc
    12961 aggctatgca gcgactgccg cgtaaccgct gtctggcgac cggatattcc tgccgtagtc
    13021 aggtcaaacg ggttgaaggc acgggggtac gccatcctgt gcaggcttta ctggagatta
    13081 ttaaatgata tggaaacgaa aaatccccct ggaagcactg aatgccatgg gtgaaggaaa
    13141 catggtgggg ttactggata ttcgctttga acatattggt gatgacaccc ttgaagcgac
    13201 aatgccagta gactcgcgga caaagcagcc tttcgggttg ctacatggag gtgcatccgt
    13261 ggtactggcc gaaagtatcg gttccgttgc cggttattta tgtaccgaag gtgagcaaaa
    13321 agtggttggt ctggaaatca atgctaacca cgtccgctcg gcacgagaag ggcgggtgcg
    13381 cggcgtatgc aaaccgttgc atctcggttc gcgtcaccag gtctggcaga ttgaaatctc
    13441 cgatgagaaa gggcgtttgt gctgttcgtc acgattgacg accgccattt tgtgacgttg
    13501 tcaccagaaa agtgtgacgc atattgcttt tggtcattat ctgcacagta aacggtggta
    13561 tactgtgcag gtcttgtcta aaagcgagga tgctatgtct actcaacttg atcccaccca
    13621 gctggccatt gaatttttac gccgtgacca gagcaatctt tcacctgccc aatatcttaa
    13681 acgtctgaaa caactagaac ttgaatttgc tgacctgctt actctctctt ctgctgaatt
    13741 aaaagaagaa atctactttg cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc
    13801 aagttcgcca aatcgacctg gattaacctc cgtccaggtt cgatgacctt cttaatactg
    13861 cttatctacc cgcctgatat tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg
    13921 cggcttagct ttatgagatt attcttatcg ccccttcaag agccaagcca ctgagagtgc
    13981 cggagataag cgccggatgg ggtagaaacc cttaagcctg tgtcgcacag acttaagggt
    14041 tttcttattt ctgaacgctg cactttttta cctatctctt aaatagtcat tttcaataca
    14101 actttttctt tttcatcaca gccttaatgc tccttagata ataatcatta tctaacaatg
    14161 agatacctaa ttcttagcgt gcctgttaac ccacacatca gggtctatgc ttattaaaag
    14221 caggcaaaaa gcatgtttta cggtaaagcc cctgcctttg ctgggttgaa ctgataatca
    14281 ttatcactaa catgctgtta tacgctgaaa gcgatgaagt gaggtaaatc gatggacatg
    14341 cattcaggaa cctttaatcc acaggatttc gcctggcaag gcttaacgct gacacccgca
    14401 gccgctgtcc acatccgcga gctggtggca aagcagccgg gtatggtcgg cgtgcgctta
    14461 ggtgtgaagc aaacgggctg cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg
    14521 gacaaagacg atctgctgtt tgaacacgac ggcgcgaagc tgtttgtccc gctgcaagcg
    14581 atgccgttta ttgatggcac ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc
    14641 aaatttcaca accctaaagc ccagaatgaa tgtggctgtg gcgaaagctt tggggtatag
    14701 gcggtactat gtctcgtaat actgaagcaa ctgacgatgt caaaacctgg accggcggcc
    14761 cgctgaatta taaagaagga ttcttcaccc agttagccac cgatgagctg gcaaagggga
    14821 taaacgaaga ggtggtgcgc gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg
    14881 agtttcgtct aaacgcctat cgcgcatggc tggagatgga agaaccgcac tggttgaaag
    14941 cgcactacga caagctgaat tatcaggatt acagctacta ctcggcacca tcgtgcggta
    15001 attgtgacga cacttgcgcg tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct
    15061 ttttaagtaa agaggtggag gcggcgtttg agcagttggg tgttcccgtg cgggaaggca
    15121 aagaggtggc ggtggatgcc attttcgact cagtttcggt tgccactact tatcgcgaaa
    15181 aactggcgga gcagggaatt attttctgtt cctttggtga ggcgatccac gatcacccgg
    15241 aactggtgcg taaatatctc ggcaccgtgg tgccggggaa tgacaacttc tttgccgcgc
    15301 ttaatgcggc ggtagtctct gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc
    15361 cgatggaact ttccacctat tttcgcatta acgcggaaaa aaccggacag tttgagcgca
    15421 ccattctggt ggccgacgaa gacagctacg tcagctacat tgaaggctgt tccgctccgg
    15481 tgcgtgacag ctatcagttg cacgcggcag tggtggaagt catcatccat aaaaacgccg
    15541 aggtgaaata ttccacggta caaaactggt tccctggcga taacaacacc ggcggtattc
    15601 tcaacttcgt caccaagcgt gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc
    15661 aatcagaaac cgggtcagcg attacgtgga aatatcccag ctgcattttg cgcggcgata
    15721 actccattgg tgagttttac tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg
    15781 gcaccaagat gatccacatc ggtaaaaaca ccaaatcgac cattatctcg aaagggatct
    15841 ctgccggaca tagccagaac agttatcgcg gcttagtgaa aatcatgccg acggcaacca
    15901 atgcgcgcaa tttcactcag tgcgactcaa tgctgattgg cgctaattgt ggggcgcata
    15961 ccttcccgta tgtcgaatgt cgcaataaca gcgcacaact ggagcacgag gcaacgacat
    16021 cacgtattgg tgaagatcaa ctgttttact gcctgcaacg cgggatcagc gaagaagacg
    16081 ccatctcgat gattgttaac ggtttctgca aagacgtgtt ctcagaactg ccgctggaat
    16141 ttgccgttga agcacaaaaa ctcctcgcca tcagtcttga acacagcgtc ggataaggaa
    16201 taaacatgtt aagtattaaa gatttacacg tcagcgtgga agataaagct atcctgcgtg
    16261 gattaagcct cgacgttcgt cccggcgaag ttcacgccat tatggggcca aacggttcgg
    16321 gcaaaagtac cttatcggca acgcttgccg ggcgagaaga ttatgaagtg acgggcggca
    16381 cggttgagtt caaaggcaaa gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag
    16441 gcatctttat ggccttccag tatccggtgg agattccagg tgtcagtaac cagtttttcc
    16501 tgcaaacggc acttaatgcg gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg
    16561 attttcagga tttgatggaa gagaaaatcg ctctcctgaa gatgccggaa gatttattaa
    16621 cccgttcggt aaacgttggt ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa
    16681 tggcggtgct ggaaccggag ttatgcattc ttgatgaatc ggactccggg ctggatatcg
    16741 acgcattaaa agtggtcgcc gatggcgtga actcgctgcg tgatggcaag cgctcattca
    16801 tcattgttac gcactaccaa cgcattctcg actacatcaa gcctgattac gttcatgtgc
    16861 tatatcaggg acgaattgtg aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc
    16921 agggttatgg ctggcttacc gaacagcagt aacgcgctgc aacagtggca tcacttgttt
    16981 gaagctgaag ggacaaaacg ctccccgcaa gcacagcagc atttacaaca attgctgcgt
    17041 accggactgc cgacacgtaa acatgaaaac tggaaatata cgccgctgga agggctgacc
    17101 aatagtcagt ttgtcagcat tgcgggagag atatccccac agcagcgaga tgccttagcg
    17161 ttaacgttag acgctgtgcg gctggtattt gtcgatggac gttacgtgcc tgcgctgagc
    17221 gatgcgactg aaggcagcgg gtatgaagtg agcattaacg acgaccgtca ggggttaccc
    17281 gatgctattc aggcggaagt gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg
    17341 catatcgccg tgaagcgcgg tcaacggccg gcaaagccat tgctgttaat gcatatcacc
    17401 cagggcgtgg caggtgaaga ggtgaacact gcccattacc gacatcatct ggagctggcg
    17461 gaaggtgccg aagcaacggt gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt
    17521 accggggcac ggttctctat caacgtcgca gcgaatgccc acttgcagca tatcaagctg
    17581 gcgtttgaaa acccgctcag tcaccacttt gcccataacg atttgttgct ggctgacgat
    17641 gccaccgcat ttagccacag tttcctgctg ggtggcgcag tgttacgaca caacaccagt
    17701 acgcaactca atggcgaaaa ctgcacgctg cggatcaata gcctggcgat gccggtgaaa
    17761 aacgaggtgt gtgatacccg tacctggctg gaacacaata aaggtttttg taacagccga
    17821 cagttgcaca aaactatcgt cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac
    17881 gtcgcgcagc acgccatcaa aacggatggt cagatgacca acaataatct gctgatgggc
    17941 aaactggcgg aagtggatac gaaaccgcag ctggaaatct atgcagatga tgtgaaatgc
    18001 agccacggcg cgacggtggg gcgtattgat gatgaacaga tgttctatct gcgctcgcgc
    18061 gggatcaatc agcaggatgc ccagcagatg atcatttacg cctttgctgc cgaactgacg
    18121 gaagcactgc gtgatgaggg gcttaaacag caggtgctga cccgaatcgg tcaacggctg
    18181 ccaggaggtg caagatgact ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt
    18241 cgcgtgaggt aaacggtttg ccgctggctt atctcgacag cgccgccagt gcgcagaaac
    18301 cgagccaggt gattgacgcc gaggccgagt tttatcgtca tggctacgcg gcggtgcatc
    18361 gcggtattca taccttaagc gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg
    18421 catcgctgtt tattaatgcc cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg
    18481 aagggatcaa tctggtcgcc aatagctggg gcaacagcaa cgtgcgggcg ggcgataaca
    18541 tcatcataag tcagatggag caccacgcta acattgttcc ctggcagatg ctttgcgcac
    18601 gcgttggcgc agagctgcgt gtgatcccgc tcaatcccga cggtacgttg caactggaga
    18661 cgctgcctac gctgtttgat gagaaaactc gcctgctggc aattactcat gtctccaacg
    18721 tgcttggcac agaaaatcca ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa
    18781 aagtgctggt ggatggcgct caggcggtga tgcatcatcc ggtggatgtt caggcgctgg
    18841 attgcgattt ttacgtgttt tccgggcata aactgtatgg ccccaccggg attggcattc
    18901 tttatgtcaa agaagccttg ctacaggaga tgccgccgtg ggaagggggc ggttctatga
    18961 tcgccaccgt cagcctgagt gaaggcacta cctggaccaa agcaccgtgg cggtttgaag
    19021 ccggtacacc caataccggg ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg
    19081 cgcaggggct taataacata gccgagtatg aacagaatct gatgcattac gcgctatcac
    19141 agctggaatc tgtaccggat ctcactctct atggcccaca aaacaggctt ggcgttattg
    19201 cttttaatct cggtaaacac cacgcctatg atgttggcag ttttctcgat aattacggca
    19261 ttgctgtgcg taccggacat cactgcgcta tgccattaat ggcctattat aacgtccctg
    19321 ctatgtgtcg ggcgtcgctg gccatgtata acacccatga agaagtggat cgtctggtga
    19381 ccggactgca acgtattcac cgtctgctgg gataacaggg aggcactatg gctttattgc
    19441 cggataaaga aaagttgctg cgtaattttt tacgctgcgc caactgggaa gagaaatatc
    19501 tctacattat tgagctgggc cagcgtctgc cagaattacg cgacgaagac agaagcccac
    19561 aaaatagcat tcagggctgc cagagtcagg tgtggattgt catgcgtcag aatgcgcagg
    19621 gaattattga attgcagggc gacagcgatg cggcgattgt gaaagggctt attgcggtcg
    19681 tctttattct ctacgatcag atgacgccgc aggatgttgt caatttcgat gtgcgtccgt
    19741 ggtttgaaaa aatggcactc acccaacatc tcaccccatc tcgttcacaa ggtctggaag
    19801 cgatgattcg cgcaattcgc gccaaagccg ctgcacttag ctaaactaaa gggacagctt
    19861 tcatccggtt ggctcacatt gccggatggt caatgtttac actgacctgc acaggagtgt
    19921 ttcggtttca ggtgaacata agcgccatcc gacgtttcag cgtgagtctc cggcaaatac
    19981 aggaggttta caatgaaacg cgcgtctttg cttacactca cgcttatcgg cgcttttagt
    20041 gccatccagg ctgcctgggc ggttgattat ccgctaccac caaccggaag ccgactggtt
    20101 gggcaaaatc aaacgtatac ggtgcaggaa ggggataaaa accttcaggc catcgcccga
    20161 cgttttgata ctgcggcaat gttgatcctt gaagccaata acactatcgc cccggtgcca
    20221 aaacccggta cgacgataac tattccttcg caactgttat tacctgatgc gccgcgtcag
    20281 gggattatcg ttaaccttgc agagctgcgc ctttattatt atccgccggg agaaaatatc
    20341 gtgcaggttt atccgatagg tattggcttg caggggctgg aaacgccagt gatggaaacg
    20401 cgtgtggggc agaaaatccc taacccaacc tggacgccta cggcaggcat tcgtcaacgt
    20461 tcgctggagc gtggcattaa attaccgcca gtcgttcctg ccggaccaaa taacccgcta
    20521 ggacgttacg cactgcgcct cgcgcatggt aatggcgaat acctcattca tggcaccagt
    20581 gcgccggaca gcgtcggttt gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat
    20641 attaaagcct tgttctccag cgtgcggacg ggaacgccgg tgaaagtgat caacgaacca
    20701 gtgaaatatt ccgtggagcc taacgggatg cgttatgttg aagtacatcg accactatcg
    20761 gcagaagaac agcagaacgt tcagacaatg ccatacacac tgccagcagg ctttacgcaa
    20821 tttaaagaca ataaggctgt agatcagaag ttagtcgata aagcgttgta tcgtcgggca
    20881 gggtatccgg ttgcggtgag cagtggagca actcccacag ccagcaatgc gccttcagta
    20941 gagtcagcgc agaatggtga accagagcaa gggaatatgt tacgcgcgac gcagtagtgg
    21001 taaatagcag acaaaaaaat ggcgcacaat gtgcgccatt tttcacttca caggtactat
    21061 tacttgcggt atttagtagc catgttgtcc agacgctggt tagcacgagc tgcgtcatct
    21121 ttagcagcct gaacgtcgga acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta
    21181 gcgttcagag tctgaacgtc agaagacagc tgatcgattt tagcgttgct ggagcaacct
    21241 gccagcagag tagaacccag gattaccgcg cccagtacca gtttagtagc tttcattatt
    21301 aataccctct agattgagtt aatctccatg tagcgttaca agtattacac aaagtttttt
    21361 atgttgagaa tatttttttg atgggaaggc acttattttt gatcgttcgc tcaaagaagc
    21421 atcgaacgct gggttttaga taaaaaaatt gagagaaaac aggatgcttc catcggattc
    21481 atcttagata aagtgagatt atatagtgaa atccgatttg attttttatt gcttctggtt
    21541 atcgattaaa taaaaaaagc gcccatcagg gcgcttcgat atacaaatta attcacaaaa
    21601 gcaatattac aggacgtgaa cagatgcggt gttagtagtg ccgctcggta ccagtgcacc
    21661 agaaaccata actacaacgt cacctttgtg tgccagaccg ctctgcagag ccagttcttt
    21721 acccagacgg tagaaatcat cagtagaagt gatctcttta accagctgcg gcacaacgcc
    21781 tttgctcagt accaactgat gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc
    21841 atccgggaag tatttacgta ctgcgcgagc agatttaccg ccctgggtag caaccacgat
    21901 cagcggagca tccagttttt cagcagtttc aacggcacca cggcataccg cttcggtaat
    21961 gcgcagttta cggttgtcat tgttgaactc gagacggctg ttcatcacgc ggtcggtacg
    22021 ttcgcagatg gtcgccatga tagaaaccgc ttccagcggg tatttacctt ttgcggattc
    22081 accagacagc atcactgcgt cagtaccgtc gaggatggcg tttgcaacgt caccggcttc
    22141 tgcgcgagtc gggcgtgggt ttttgatcat ggaatccagc atctgggtcg cagtgataac
    22201 gactttacgt gcacggatac atttttcgat catcatcttc tgggcgaaga taacttcttc
    22261 taccgggatt tctacaccca ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag
    22321 gatttcgtcg aagttgttga ggccttcctg gttttcgatt ttggagatga tgtggatgtt
    22381 ttcgccgccg tgcgctttca ggtgctcacg gatttcgata acgtcagaac gcttacgaat
    22441 aaaggaagca gcgacaaagt ctacgccttg ttcgcaacca aagatcaggt cctgtttgtc
    22501 tttttcagcc agtgctggca gggcaatgga aacgccaggc aggttcacac ctttgttttc
    22561 acccaggtcg ccgttgttca gcactttaca gataactttg ttaccttcaa tggcagtaac
    22621 ttccataccg atcagaccat cgtcaaccag tacggtgttg ccaacagaca ggtcagttgt
    22681 gaaaccttca tacgttaccg caaccatttc gctgttgccg ataacagatt tatcagtagt
    22741 gaaagtaaag gtctgaccag ctttcagaga aacgtcgtta ccgccttcca gtttcatggt
    22801 gcggatttcc ggacctttgg tatcaagcag gatagcggcg gttttaccag ttttgctcat
    22861 cacgttgcgc agattctgaa tgcgctgacc gtgttctgca tagtcaccat gagagaagtt
    22921 cagacgcata acgttcatgc cagcgtccag cattttagct aacatctctt cagattcggt
    22981 tttcggtccg atggtgcaaa caattttggt ctttttcatg acagtcttag tctttaagtt
    23041 gagaaggatg ggagaaactt gctttctggg cgcactgccg gaaagcgaat ccggtattac
    23101 gaaagtggtg gtgcctcgct ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt
    23161 gtgcttgtgt ctcagcccaa cgtgagtgcc tgtttttgag tcgttgagtt ctacagtcca
    23221 aagtgctgaa accattcaag agtcaattgg cgcgcattat acgctgaaat taatccaaaa
    23281 ggaaaacaaa aacagcgttt caaaaaatat acaacttgtt agacaataaa ttgttatcaa
    23341 agatttaacg ttccagggaa aaggttacgc aaaaacttcg ctgtttgcgc aacgtctggt
    23401 gccggggttg tcactgtgac gataagtgaa agttcatccg gtgtataaat acatcatcgc
    23461 tcaattgcta cagttaaaat aatttatcgc gttatttgtc tgattttgtt tgatttttgt
    23521 ttaattggtt agattgatat atgctgatag ttggcatgat aaataggtat aaagaatggg
    23581 aaatcggaca aaagaggatg aactgtaccg agaaatgtgc agagttgtcg gtaaagttgt
    23641 gctggaaatg cgcgatctgg ggcaggaacc aaagcatatt gttatcgcag gcgtattacg
    23701 tacagcatta gccaataaac gcattcagcg cagtgagctg gaaaagcagg cgatggaaac
    23761 cgtcattaac gcactggtaa aatgaccatt caggatgagg cgataagctt catcctgata
    23821 ttcttgcttc agttgcggtc cagttaatca ttctaaataa caactgctga
//
LOCUS       DAZKIF010000074        23861 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZKIF010000074 DAZKIF010000000
VERSION     DAZKIF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.074"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(8..160)
                     /locus_tag="ACHOTW_002833"
                     /pseudo
     CDS             complement(8..160)
                     /locus_tag="ACHOTW_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(157..277)
                     /locus_tag="ACHOTW_002834"
                     /pseudo
     CDS             complement(157..277)
                     /locus_tag="ACHOTW_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(317..580)
                     /locus_tag="ACHOTW_002835"
                     /pseudo
     CDS             complement(317..580)
                     /locus_tag="ACHOTW_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            674..1023
                     /locus_tag="ACHOTW_002836"
                     /pseudo
     CDS             674..1023
                     /locus_tag="ACHOTW_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1688..2119)
                     /locus_tag="ACHOTW_002837"
     CDS             complement(1688..2119)
                     /locus_tag="ACHOTW_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFP9844906.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(2187..4208)
                     /locus_tag="ACHOTW_002838"
                     /pseudo
     CDS             complement(2187..4208)
                     /locus_tag="ACHOTW_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4342..4467)
                     /locus_tag="ACHOTW_002839"
     CDS             complement(4342..4467)
                     /locus_tag="ACHOTW_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFP9844907.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(4409..4558)
                     /locus_tag="ACHOTW_002840"
                     /pseudo
     CDS             complement(4409..4558)
                     /locus_tag="ACHOTW_002840"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(4732..5493)
                     /locus_tag="ACHOTW_002841"
     CDS             complement(4732..5493)
                     /locus_tag="ACHOTW_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP9844908.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            complement(5490..5924)
                     /gene="psiB"
                     /locus_tag="ACHOTW_002842"
     CDS             complement(5490..5924)
                     /gene="psiB"
                     /locus_tag="ACHOTW_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP9844909.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(5979..7937)
                     /locus_tag="ACHOTW_002843"
     CDS             complement(5979..7937)
                     /locus_tag="ACHOTW_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9844910.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            complement(8003..8236)
                     /locus_tag="ACHOTW_002844"
     CDS             complement(8003..8236)
                     /locus_tag="ACHOTW_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP9844911.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            complement(8293..8745)
                     /locus_tag="ACHOTW_002845"
     CDS             complement(8293..8745)
                     /locus_tag="ACHOTW_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9844912.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            complement(8771..8977)
                     /locus_tag="ACHOTW_002846"
     CDS             complement(8771..8977)
                     /locus_tag="ACHOTW_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9844913.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            9047..9496
                     /locus_tag="ACHOTW_002847"
     CDS             9047..9496
                     /locus_tag="ACHOTW_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844914.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASPGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQENNKTDSNQSSR
                     KKKNSSSSSSSSSSSSMNNNIISNRQMTGLKRQKPTAECPRNVRGGA"
     gene            complement(9582..10145)
                     /locus_tag="ACHOTW_002848"
     CDS             complement(9582..10145)
                     /locus_tag="ACHOTW_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP9844915.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(10192..11553)
                     /locus_tag="ACHOTW_002849"
     CDS             complement(10192..11553)
                     /locus_tag="ACHOTW_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFP9844916.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(11605..11835)
                     /locus_tag="ACHOTW_002850"
     CDS             complement(11605..11835)
                     /locus_tag="ACHOTW_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844917.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            12352..12861
                     /locus_tag="ACHOTW_002851"
     CDS             12352..12861
                     /locus_tag="ACHOTW_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844918.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            complement(12831..13022)
                     /locus_tag="ACHOTW_002852"
     CDS             complement(12831..13022)
                     /locus_tag="ACHOTW_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844919.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(13019..13441)
                     /locus_tag="ACHOTW_002853"
     CDS             complement(13019..13441)
                     /locus_tag="ACHOTW_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP9844920.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(13488..13913)
                     /locus_tag="ACHOTW_002854"
     CDS             complement(13488..13913)
                     /locus_tag="ACHOTW_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9844921.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(14331..15107)
                     /locus_tag="ACHOTW_002855"
     CDS             complement(14331..15107)
                     /locus_tag="ACHOTW_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844922.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESAHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(15153..15707)
                     /locus_tag="ACHOTW_002856"
     CDS             complement(15153..15707)
                     /locus_tag="ACHOTW_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP9844923.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(15601..15822)
                     /locus_tag="ACHOTW_002857"
     CDS             complement(15601..15822)
                     /locus_tag="ACHOTW_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844924.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(15823..16506)
                     /locus_tag="ACHOTW_002858"
     CDS             complement(15823..16506)
                     /locus_tag="ACHOTW_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFP9844925.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(16583..16888)
                     /locus_tag="ACHOTW_002859"
     CDS             complement(16583..16888)
                     /locus_tag="ACHOTW_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844926.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(16892..17794)
                     /locus_tag="ACHOTW_002860"
     CDS             complement(16892..17794)
                     /locus_tag="ACHOTW_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP9844927.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(17871..19595)
                     /gene="ltrA"
                     /locus_tag="ACHOTW_002861"
     CDS             complement(17871..19595)
                     /gene="ltrA"
                     /locus_tag="ACHOTW_002861"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9844928.1"
                     /translation="MTEQATTCKGASLLNGDSWHSINWRQCYREVRRLQARIVKATRE
                     GKHGKVKSLQWILTHSFSGRAVAVRRVTENSGKRTPGVDGQTWSSPEVKFLAINLLKR
                     RGYKPQPLKRVYIPKSNGKSRPLGIPTMKDRAMQALYLLALEPVAEVTADQRSFGFRT
                     GRSTADAIAQCFCVLAQKTSAEWVLEGDIRGCFDNISHQWLIDNTSTDRQILTKWLKA
                     GYREKGQLFPVNSGTPQGGIISPVLANIALDGLEALLASEFKKRTVKGRLVNPKVNYV
                     RYADDFIITGESKELLESQVLPVVRRFMAERGLMLSPEKTKITHIEEGFDFLGQNIRK
                     YGGKMLIKPSKANVSSFLKKIRAVIKGNKAMDQLTLIRMLNPMIKGWAAYHQHIVAKV
                     AFNKVDNEIWLALWRWAVRRHPNKGKKWIRKRYFHQQGARNWSFSTATGELLANGKPQ
                     YANLRKAIDTPINRFKPIKIAANPFDPQWEMYFEERCADKMRHKLQGRKKLIQIWFEQ
                     RGRCPICDERITSDSQWQVHHIIRRVDGGSNCLSNLIMLHPMCHTLVHAKGIHVVKPA
                     HESGLRKA"
     gene            complement(20895..21866)
                     /locus_tag="ACHOTW_002862"
     CDS             complement(20895..21866)
                     /locus_tag="ACHOTW_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFP9844929.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(21866..23032)
                     /gene="sopA"
                     /locus_tag="ACHOTW_002863"
     CDS             complement(21866..23032)
                     /gene="sopA"
                     /locus_tag="ACHOTW_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFP9844930.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(23620..>23861)
                     /locus_tag="ACHOTW_002864"
     CDS             complement(23620..>23861)
                     /locus_tag="ACHOTW_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9844931.1"
                     /translation="RKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEI
                     NSRTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
BASE COUNT         5480 a         6894 c         5923 g         5564 t
ORIGIN      
        1 catccaatta gtagaacatg tgtttttcga taaacgctcc gatcactttt tcgtggatct
       61 caactgagcg tgagaagcag atggttttac gagtcaaccg cttaatgcgg gtgcgtagcg
      121 tcagattatt acgctcaatg cgttgggtga atattttgcc agcacgttat caccgaacat
      181 ctctccccca gtcggcgaac cctttattgg acgtcagcac aatgctcgct ttttcatatc
      241 gacggttcag caaccggaag aacagactgt gttcctgcgt aaagcgctgc tccggtgtct
      301 tcctgaactg gcgaagcaac atggaacggc caccggtata acccatcgct ttgatttccg
      361 ccaggataac ttcgccgttc cagacattct cagccagacg catgtcgata tagtccattt
      421 cggccatttt gtggcgtgtt tttctggccg gaggttctgg gtatttcagg taacgtctga
      481 ccgttcgttc agagcaacca atctgagtcg caatatcaac aatatacgca ccttgctggc
      541 gcatttgctt tatcatgtaa aagtcctctc tgctcagcat gctgatgtcc tttctggtgt
      601 gagaacctca aggaaacaac atgttgggtg gagcggacaa tccaaatggt gaattaccgt
      661 cttatatcac tggcggataa ggataagtac acgtcggcag ttccgtcagc actgcgacag
      721 cgttgtcctc gttgctttta cccggtcatg cacaacgcct gatggatgaa ctgcgtgccc
      781 agggttacca ttttaaagta aaaaccgtga cggaaagcct gcgccgtcac ggactgaggg
      841 caaaagcttc ctggaatttc agtccggtct gttaccgcgc acacagccag cctgtgtcag
      901 aaaatctgct ggagcaggat ttttacgcca gtggcccgaa ccagaagtgg gcgggagaca
      961 tcacatattt acgtacggat gaaggctggc cgtatctggc tgtggtcacg tgccattatt
     1021 ggctgatcaa tgtcgtcacg catgacggca caggagcccc cggaacgtta tgctaccgca
     1081 tgtcatttgc aaaaatgaca gatagatata cttcaatttg aatcttaaca attagcatgg
     1141 gaatgttgtc aatcctctgt atggagccac cacatattgc aatttttgtt ctcttttaaa
     1201 atatattcca cgagcgtctt gtgggacctt ttgctgttat aggggggcag tttggatatc
     1261 gaaaattatt gtacgttaag gctgaatttc aatcgcaaag cataacctta cctttctgct
     1321 tacttatgca gttcaaagag taccaactct gcaattttcg ttgccacaga tgcaatatcg
     1381 ttactttcca gtcaccatgc cgctgaaagc ccacaccatg cgataatcca catcaagagc
     1441 cgctttctgt ccagacccgc tgttgtgagg tgtgaggtgt gaggtgtgag gtgtgaggtg
     1501 tgaggtgtga ggtgtgaggt gtgaggtagc ctgagtttaa cggacaggcc gatgaaggac
     1561 gccgttaccg ggataatgta agaatctttt tttgagtatg tcaacataac ctgaggcaaa
     1621 gtacctcagg ttccagtaat cagccgacct gacagtcttc tgttagccct acagatatgt
     1681 cgtgtagcta accgcgcatg tcaaagccgg atttttgaaa atctctataa acttccggat
     1741 tgttgaaggc cgggaattta gctttatggg ctgctgactt tatcgcttcg caataggctt
     1801 tatcgccatt actggttgat atttttaacg ccgtgccatc ccgggagaat tccatatgca
     1861 acctgcattt tttccttttc catttttgcg gatcatcaag tttagcatta attgcatccc
     1921 tgattccccg tgcctgctcc ccccattcat cctgatcatc ccaacgtcct gaactgcaac
     1981 tacccagggc tgtagtttta tggcaatttg aaggatgtaa tggcgcacat cccgccacaa
     2041 agctgactaa aatagccagt ataacgattt tctttacccc catatattgc tcctcaaccc
     2101 atatagatct cagtaccata gctctgtcag agcaaagaaa atcttaacta atacaagtta
     2161 agatttttaa tatccactcc tcatccctac attccaggtg atggtggcgt ccactcatta
     2221 tcctgaactg gagcataaga tgcgccatgc cccccggata tgcttttctg ttgcctgata
     2281 tattcgtggt agtactgttc actacgctga cgatgtaaat ccacatccgg gcggctgatt
     2341 acaaagtgtc catttccggt tattccagct gcattatgcc aggccaccat ttccgcaaat
     2401 acatcattaa actgttgata tgagccttcc ggttcaaaac ccattcctgg ctcacgatca
     2461 aacaatacag gagcaatcag attcagctta tccaccagtt catttctgtc ctgatgatgg
     2521 tagaacacca cacggtcccc ataatccaca cagactaact cgccattcct ccgatcacga
     2581 aaacgcacac tgccatcttt ctgcagacgg gcttcgagaa cgccagtgtc ttcataaagt
     2641 ggagttcctc ccggttcaca gagaatcacg cagcggtcgg cattcgttgt tcgtcgcttg
     2701 tctttgcgac gatcacgata atcccagcca cgtaactgtg ataatgcagc tctgtctccc
     2761 tgtaccgcct gttgctccac ccactgccgg taggacaaag gataccactt accctcctca
     2821 atcagtgaca accgctcttc cttcacactc tccttcagcc tgatcagcgc ctgcatacgc
     2881 tgcacttccg caatatggta atgcagctta cgcaattgtg gatcccggaa ctgcacacgg
     2941 atatacgcct tgcgccgacg gcaggcagca tgaatttccc gtaaccgttc tccgtaacgt
     3001 aagtcgggtt tacgccagtg ctccttccat gcaagataac gcgcgcgcaa atcttcccgc
     3061 gcagctgcac gggcttcacg gcgttcacga cgcagtacag gatcgcgccg gaagccgggc
     3121 tccacttcat cgctggcagc aagttccgga ttgtagcgac attcaggctt cacccgttca
     3181 aatatatctg cacctgcaat ctcaaatggc cctgcctgcg gctcagccct ggataacgtc
     3241 aaatccgggt ggatactgct ggccttcact ggtgtgagct catgattaaa cgcatccaca
     3301 atcacaagac catgatgctg tttctgcaac ataagccctt tacgcgcaaa catctcatgg
     3361 cacgcctgcc agctgaaatt cccgcccgcg atcatatctt caatcaacga ctcacactga
     3421 acagttgtgt acccccacag actttcctta tcagaaaaca gttcgcgctt tattgctccc
     3481 tgtggacctg tccccatccg catacgatac cattcgtaat cactgacagc ctgagtacca
     3541 agattacgtc cccggaaccg ttctccgggt ataacctgag taaaaatatc cttcggtaca
     3601 ggctgccatc caccatccag atcctgcata taatccagcg tcattaacgg ccacacactg
     3661 tctgcacgta ctggtgtctg ttgtttatca aatccatcat gcagtaccag atgcccgtgc
     3721 tggatcccca tccataatcc agccgttgcc agagttctgt gcacatccag gcaactttcc
     3781 agctcaccat ttctcgccat ttccggtaag cgttcggcaa aatgacggca ggcgtactgt
     3841 ttcagactct ccgtttctgc cactttttcc ggccggatat caacgtttat ggcttccgga
     3901 tcataacgac cgggtgtccc cacctgactg aaaatatctg tcggtacagg ctgatattcc
     3961 cccagtttcc gccccagttt ttcagctgtc cataacggtc cgaacgaact gagtgcaaca
     4021 ccttcccgcg ctctgtccca gccatccttc accaccagtt ccccttcctg catcgtgata
     4081 tacagaccat cactggccag ccgtttatgc aaagataacc agtcctgtgc gggctcttca
     4141 cgcagcccgg caatcgacat ctgagcaata tattccctga acgtctgctt tttcccgcgt
     4201 cgccaggctg ttcatgcccg tacaatgcca gcaaggagaa aggggccacc ggcgaaccag
     4261 cagccccttt ataaaggcgc ttcagtgcag tcagaccagc atcagccctg aaaaggcggg
     4321 cctgcgcccg cctccaggtt gctacttacc ggattcgtaa gccatgaaag ccgccacctc
     4381 cctgtatccg tctctgtaac gaatctcgca cagcgatttt cgtgtcagat aagtgaatat
     4441 caacagtgtg agacacacta tcaacacaca ccagacaaga gagctgcgtg gtagtttcat
     4501 ggccttcttc tccttgcgca aagcgcggta agaggctatc ctgatgttgt ctaagcatgc
     4561 aggggcctcg tgggttaatg aaaaattaac tacggggctt ttgtccttct gccacacaac
     4621 acggtaacaa accaccttca cgtcatgagg cagaaagcct caagcgccgg gcacatcata
     4681 gcccatatac cagcacgctg accacattca cttttcctga aaataacccg ctcattcagg
     4741 ctgtttacgg gcaatccgtg tgattgttgc cgcatcacgc acctcccgga gtttgttcgg
     4801 caccatccac cgctccatct cacggacagc atgaccggcc tcctttgctg ccagactggc
     4861 ttccgcgata acctgccaga atggcatatc ctgccactgc cagcgttcca ttccggccag
     4921 tgacggaaaa cgctcgcccc actgccagaa cagggtttcc aggtcatgct ctgccacccg
     4981 atcactccag cgtgcatacc agcttccgac tgtttccggt gaatggaacg ccagttcgat
     5041 ttcagcctgg gcgaccagcg cctggtacgc acgggctttc tgctctgcct ccctcgcctg
     5101 ctggcggctg tacttctgca tgaccagcgc ggatttatgg cgactgcgtt ccctgacaca
     5161 aaaccgcacc gacgggaaca agcgttctgc tgcatcaccg ggaaggggaa ggaggcagac
     5221 ctcgccctgt gtttcaatca gcacatcgat ggcatacaac cactgaagac ggtttccgtg
     5281 cagttcttct gcggtcaggg acggcatgaa aaatcgcagg tcagacagcg atatcctgcg
     5341 gcttccgttc agacagcgga aaaacgcccc cgcatacgga tattccgcca gcgtgtttcc
     5401 ctgattgcgg cgtttttccg tctctgccac tgcccgccat gcggcctgtt gctctgtgct
     5461 gaggggaacc agtgcctgtg aacgtgcgct catacctgac ctccttccgc catcaggata
     5521 tggatgatgt cgttaacgct gtatccctgc gtatccagtg acgccgccag tgccagtgaa
     5581 tggctgacgg cttccggtgc aaaccgccgc tgtacctgaa ccaccgcaaa cggctcacca
     5641 ccggcattca ccagaaccag cacccagacc tgggagacat cgcccggtga acataacgcc
     5701 aggtgaaaac gttcgtgggc aggcgtgaaa cggacctgga cggggaaaaa gccgccaaac
     5761 tcgctgccgt actcgccgtt catcgtccag ttgtccggcg catccagctc ccgcatcacg
     5821 gcgtgtgtca gctcacggcg tgcatcactt ccggcagccc ggatatcttc atattcctgt
     5881 gcgttcatgg tatgtaatac attcagagtc agttcagttt tcatcacggt atctcccttg
     5941 ctgccgcccc gtctccggcg gggcggtgtg gttgttcatc aggcggcatc agccaggtta
     6001 tcggtgcgtt cagtggcatc cgtctgtggg cgtggtgcac acatccagcc aggcacccag
     6061 cggttgtctt tcatatggtg ctctgcatgt tcagccgcat cgcctttctt catcttctcc
     6121 gcgtcccgtg cggcaccgga tagtcctgcc tcattcaggg ctgcgataat ctgcggcttt
     6181 ttcaggtgtc cgaaaaagtt tgctttcgtc ggctgccacc agtcgcgcat gtgaaagccg
     6241 atggcgctct ccagcgtgtc cagcggactg cgtgacgtgt gaccatactc acgggtctgg
     6301 acaccgtgga tactgcacgc ggtgcagaaa ctgagcagtg ataacagcac ctcctgactg
     6361 agtgacagga acgtcgtcat atcccgtgcc catccatccg gcagcagggc ggcaagacgg
     6421 gctttttctg ccatcatcgc catgaatgcg gcaccttcct tccctgacgg cgcatcgctg
     6481 gtcagcgaat aatgctcaca ttccaggcgg attctggcgg gattgctgta cgctccactg
     6541 ccaaatacat tcagacagag tgtccatgcc agaagtgcca gggatttgtc tgattgctgc
     6601 atcagtgccg cctggactgc cagcgtgcgc tctgaggaca ttttcgtcag cagcggcaga
     6661 ctgatttcct ccactgaggc tttctcctgc acctgttccg tgcggtaatc gttatcagcc
     6721 acatcatcct cactgcgcaa ctgcacacca cgctggacac acacgttgcc ataacgccag
     6781 gacaccacca cgccacttcc ggcacgcatc tccggcgtcc acgccctgac cttcgccatg
     6841 cagcgcatca gcttcatttc ggcttccagc aggtcggatt cctcacactg gttttccagc
     6901 gcatcgtaac gtgccatcag ttcgttcagg cgttcttctt ctgcctccgt cagcaccgct
     6961 tccggctccg gcagactgcg gtatgttccg gcatcctcac ggcagaaacc aacaggctcc
     7021 atacgtccgg cgcaccattc ccagccttcg gcttcccgaa ggtgttcggc gacagcctgg
     7081 agtttttcca gcagggcggc atcgagcgcc acgcagtcca catagccacc ctcgtcgtca
     7141 ctgaacaaat cggtgcgcag ttcatctggc gagaaggtat cagcccccac gaagcggaat
     7201 ttactgttcc ctgccaccgc cacttcactt tcggtcacca gacgacgaat ggtctgtact
     7261 tccggtttac cgccccatcc cgactgacag gcggcttcaa acacctgcac ctgacgcgcg
     7321 gtgtcgttct ccagcgccag cgcctgacag tgctcggtgg tgatgcggtc ttctgccagc
     7381 gcatcgagga tgacaggcgc aaggtctgcc agtttcagca ttcgctgaac gtggcggggc
     7441 gaataaccca gcaaatcacc gatttgtgca ggtgttttgc cttcctgcgc cattgcacgg
     7501 aatccggcaa tctgttcggc agggtgcata tcccgacgat gaccgttctc ggtcatcgat
     7561 gcggcagtcg ccagctcctg cggaatgacc ttcacacgga caggccagtc agccggaatg
     7621 atgtcacgct ctgccagcat gttgagtgct gccagtcggc gaccacctgc ggcgacaccg
     7681 taacggtcac ctggcagggc atgaacaacc agattctgta acaggccgac acctttaatg
     7741 gagtccgcca gttcgctgac ggactccgca gaatacggca ccgtgcgcac attcagcggt
     7801 gacttaatca gcgaggccag cggcacgctc acttcctccg tctgcgccag cagggccgac
     7861 aggactgttt cctgcgtttt tgcaggttta cggctggatt tacgctccgt ttttgcctta
     7921 gactctgtaa ctgacatagt tagtacctcc caaaacggta ataatgattg tctgccggac
     7981 gcgttcccgt ccggcacctc tcttacttaa taatcccgaa atcacggatg acatggttca
     8041 tccagtaccc ggcaccgtcc tcaaaattcc aggtacgcca gaccattgca ccgttattac
     8101 ggacaaccag gcgaaactgt tcgccgtggt cattctcgat aaagacgttc cggtactgtg
     8161 cggcaacggc ttcggcatgt ttgcgggtaa aaggaccgtc aggcagtccc tgaagtattc
     8221 tgaaatattc gctcatcctc atctctccgg tgatgccccg cacaggacgg ggcggttgtc
     8281 acagtcagtc gttcagaacg ggatatcgtc tgaaaacccg taatcgtcac cctccggcgg
     8341 ttgcggctga ggctgaccgc tgaactgttg cccctcttcc ggctgagtct gagcacctgc
     8401 ggcacgtacc agcatctgca tggtgcccgt ggtcttaaca agaatttcgg tgacgtaacg
     8461 ggtgataccg ttatcctccc agctacgggt gcgaagctga ccttcgatgt agacctgcgc
     8521 gcccttgcgc agatattcac ctgccacttc cgcgagcttg ccgaacagca ccacgcgatg
     8581 ccattctgtc tgctcccgca tctcccccgt ctgtttgtca cgccagcttt ctgacgtggc
     8641 cacctgcagg tttgccactg cgcccccgtt ggggatgtaa cggacttccg gatcttttcc
     8701 caggcgtcct acgaggatga ccttgttaat gccacgaact gccatgatgt gtctccttct
     8761 gttgatgttt tcagccccca tcatcttttg agtttcggga gccgcggttt tgacgttttg
     8821 cggggacctg ccttttgttc cctccggtat tcaccatcac ctttttgaga accttcgccg
     8881 caaggggcat cactgccacg gccggaaacg actgtccggg ggcagaccgc aaaacttttt
     8941 gcctgctttt gggcaaaaag ttttgcggga tgtgcatttc acccctggac tggcggagac
     9001 ggttgtggct gtctttgccc ccggcgacag gggcttaaac aggtgaatgg caccggacac
     9061 aacaacgggc aggcacccgc cgcaacgacg tgaagcgcgg tttgcgaaac ggcgttgccc
     9121 cttacatccc ggcggccttt tgccgggatg ccgggagcgc aggccacggc gcgacagagg
     9181 cggcgcgacc ggaacggtcg ctgcaagccc gggtttcgcg tcagggatgg aagcccgcca
     9241 gggcggagac agctccgctg gctccgttca cgacagcccg ccccgccagg gggacgcccg
     9301 gaaaaggcat aagcaggaga acaacaaaac agacagtaat cagagcagca gaaaaaagaa
     9361 gaacagtagc agtagcagta gcagtagcag tagcagtagc atgaacaaca acatcatcag
     9421 taacagacaa atgacaggtc tgaaaagaca gaagcccaca gcagaatgtc cgcgtaatgt
     9481 aaggggcggc gcgtgagcgc ggcccctttc agcgccaccg gcgcgcggtc ttttcccggc
     9541 gcgggcagac gggcatttat cctcccgcct gcccggagtc atcaggcacg cagacgaata
     9601 atcatcgtcg gaacatcggt ataagcaaac gtgccgcgtg gcagctcctc gcggacgtca
     9661 gaaaacggta acagcttctc ctgctggcgc ggcccgttca gacagacggc gaccagcacg
     9721 ccacccggac gcaacaggga aaaggcccgc aggatatgcc ggatatcctg cccgtggctg
     9781 aacggcggat taatgataac ccggctgtaa tactgcaccg gctgccattc catgaagtcg
     9841 ccacactgaa ccctgacacc gttaaaattt tcccgcagat accggaccag cccgctgttg
     9901 atttccaccg catcacacat ggcctccggt gcggtatccc gaatcgcccg cagtatggcc
     9961 ccggtgccgg cagagggttc cagaatgtga tcacggttgc tgatctccgc cagcgtcacc
    10021 aggcgatcac agaccggggc aggagtcaca aaaagctgag attcagcccg gatttcagca
    10081 accggcgcgg cgttatccag ccttgcgcgc atcagccgga tacgttcttt taactgactg
    10141 tgcatgtcat tatctccgtc atcccccggg gatttcccgg ggggatatac cttatttctg
    10201 tgggatttcc gtaattttca tgtcggtgat atacacattc accagacgga aattattgtc
    10261 cattgtgcgg cggtagcggt atgccccgtg gtcttctgct tctgccacac tgcgcacggc
    10321 cttacagtcc cggggcaggg cggcccactg tgcctttgtc atttcccgga aaccttcccc
    10381 cggatagttg actaccggcg gacgcttcgc ggcctggctg gcgacggcag cctcttctgc
    10441 cgatggtgct ttgtagtccg tgatgcgatc gggcgtcact ttcatcgtgc cgctgtaccc
    10501 gagaaaactg taattcggcg ttgtgactga actcaccgcc ccgttgcttt tgttcacgcg
    10561 gatgatggtc agccactcgc cccggctgaa aacctgtccg cccggctcaa aatcctgtgt
    10621 ccgggtaacc acgccgccgc tttcgtccag catggcacgc tcatagttca ggcggttctg
    10681 atagtggtta acccagcgtt gctgatgctg aatctgccgt tcatgacagc ggatcgcaat
    10741 ttcgcgggcc tgctccgtgg tgatgatgtc atcatccagg gccgaccata atgaccggct
    10801 cccttcatac tggctttttt ctgccgggcg cggatacgta tccagcggga aacaggcgct
    10861 gatatggtca tgactgctga tgagttttgc catattcaga tccagcgatt cagcccgcca
    10921 catcgtcaga tatttctgcg actgcgcgat ggtcttttca gccttgcgca aatcagcttc
    10981 gatttttttg atacgacgcc agcgaacgtc cggacgttct ttatacttcg cgtgaagcag
    11041 tgctgaccgc ccccgttctt cccagtattc cgcacgttca aagagcatca cggcacgttt
    11101 catgccgttt tcaatacgct gcgcatcacg acgggcgcgg cgttcgctgt gatgccccac
    11161 aagaatgggc tgaccgggcg ggatcatcgc ggccagtctt tccacttcat caagtgcctg
    11221 tgcggattca ctggcccgct ttccgctgta tccggtaaac cgctccgccc gtgcttcctg
    11281 acgttcagcg agcgtgctgt cttcatcctc aatctctccg gcaagtgaga ggagcacgtc
    11341 ttcccggccc ggtgtccagg ccggcgcgac aaacagcgcc tgttttggtg cccagcgaaa
    11401 accggcatca tgcacttttt tatacgtctc cgggtcaaga cgtgacacgg catacaggcg
    11461 cagtttgttg tcatccggtg agtaggtcgc gcggtacatc tgcacagggc tggcggcttc
    11521 cggggcggtc atggtgattg ctgctgatgt cataaaatgt tctccttccg tctgttgccg
    11581 cccctcgcgg ggcggctcac ctcatcagtt aaaagcgcgt ttcagttcga gtgtgcggag
    11641 cacctgcccg aaagccgtgt ttttcatggc ggggatgtgt acaccacgcc cccggtgaaa
    11701 actccgggct atgtgtcgcc cgaaggcttt cagcacgggg tcaatttccg gagcggggat
    11761 gtcttcgcgt ctgaaacggg gatgacggcg gggctttaca gccgctgcgg aaggtgaaac
    11821 aatgcttaca gtcataaatg gattccttat gacaaattgg ttaacaatgc cgtcaaaatt
    11881 ggtcgcacaa ttcttacggc gtggattaag caggtccctg acctgctttt ttcatatctg
    11941 catctgacgc tttccggtgt tttcctcctt ctgttcctgc gttctcatta cctgtcagcc
    12001 ggatgactga cagaacacat aaatgcgtat tgccggagcc tgaaataaat cctgattttt
    12061 aaagaacatc acccgttgag tttcgggagc cgcggttttg acgtttcgcg ggaacctgct
    12121 gctgtattcc ctgtggtatg aacttcaccc gtttgagaac cttcgccgca aggggcatca
    12181 ctgccacggc ctgacacacc tgtcctgggc agaacgcaaa attttttacc gggttctggg
    12241 taaaaaattt tgtgggctgt tcatttaccc tggacagtca gtgcggcagg ttgtggctgt
    12301 atcagctccc ggcgacaggg actcatacgg gtgtgtggca ccctgcaggc aatgagcagg
    12361 gacccgccgc aacgacgtga agcgcggttt gcgaaacggc gttgcccctt acatcccggc
    12421 ggccttttgc cgggatgccg ggagcgcagg ccacggcgcg acagaggctg cgcgaccgga
    12481 acggtcgctg caagccaggg tttcgcgtca gggatggaag cccgccaggg cggagacagc
    12541 ttcgctggct ccgttcacga cagcccgccc cgccaggggg acgcccggaa aagccataag
    12601 caggagagga acggaaagaa gaggaggaag aggaagagga aaaagaagaa gaacagtaac
    12661 aggcacggta accacagaaa aataacatca gaaaaaaaca ttccggacac atcacaaaca
    12721 ggaagggtaa cggacgacgc gtcagcgtgg cccgcttccc ttccggcagg cgtcagcctg
    12781 ccaggggttt tcgcgcgggc tgacaggcgg cctccaccgc cagccctgta tcacctgttt
    12841 ctgctgccct gtaagatgta gtgaccgccc aggaaaacag cctgctgatt cgccacaaaa
    12901 tcgcagattt catcaacaat ctgcgcggtg gtgagcggtt tctgccccat ttcacgacgt
    12961 cgcgcattaa tcacggcttt gtaattgcgc agaatttcgc gggtttctgt gctgatattc
    13021 atgacatgtg ctccccgtcc agcgcctgta acacaacgtc cattgcttcg cgttcctccc
    13081 tgacgaacgc atcaccgtcg cccccgttct cactcccgac ggcatacagg cgcttcattt
    13141 cactgcctgc cagcgccatc actttttcca gcatcaccgc cggaactgtc acctgacggc
    13201 tggcacgctt ttcttccatc aactcacgaa caacgtcgtg aacaatgctg acatccgcac
    13261 catattcaaa ggcatcgtcc agccgctgca tgacggtttc cagctcatcg tctgacaggt
    13321 tccagtcctg ggctatgtgg cgcacatccc cacgggtcag caccacggca aaaacgcatt
    13381 cactgtccgg cacgtctgta tccagtacat cgcggatgat ttctttaaca gtacagtaca
    13441 tgttctcgtc tcctttacag gggcggcatt gccgccccgt ccgttaatca gtccagtgcc
    13501 gcgtaaatcg cgttgcattc agggtgaaat tcgatgtgac gccacaactg cgcatcgcgc
    13561 atccggtaaa gctgggtcag tcctgcatca ccgctgtcgt ggtacagcca caactggcgg
    13621 ttaatcacaa gggaagtcag aatgatgccg caggcatcag cactcacggt acggtcaaac
    13681 cagtttgcac cgttcaccat gtggaaacgg tcgccatccg gcatcatgta accgccaccg
    13741 tccggcaggc ggatgaagtg ccagaagcct ccctgatatt ccggcatcat tttttgcgcc
    13801 agcgcgtaca catacgcctc ggcatacaga aaatcgttgc caaacaggta aggcagaaag
    13861 gagagacggt cgcactcttc aacattcaga gtgacaggtt tcgcatattg catgatgttt
    13921 tcctccgttg ttcacatatt ccgcgacacc gggcggtgcc gtgcctccag aaaagccggg
    13981 cgataaggcg gggatgcaca gggcgcaagg gaagccgcag cctgacgcac acggctgacg
    14041 ggagttgcag ggtgaggccg cgaaacctga gcgggcgaac gaccggaaaa tcacggcaga
    14101 cgggaaggag ggaccgggtg accgcagcgg cgaagcgtac ccttgcatca acgccagccc
    14161 ggctaagatg gaggcacggc accgcccgca cacgacgctg aggccacata actgaaaaac
    14221 cacaaggcca tgccctgcat ggccgggtaa agcggagcca gcggcgactt cctttgtgct
    14281 ctgtccgggg accggcagga aaaaggcggg gatttacccc gccaacgtca tcatttccgg
    14341 atttttgcca gggcatccgc catgctgcgg atttcgttcc gggcttcccg gaccacctcc
    14401 cgcagaacac cacggtcaaa ccaccggcgg ggcggttcat ccggtatacg cagcacatcg
    14461 caggccagcc cactggacag gatatgttca atacaatcgc tgaccagata catttccacc
    14521 gccgaaaaac gtgcgccccg gttcaggatg gcatcgagca ccacgggcag gttttcgtcg
    14581 tcctcatcga gccagcaggg gtgagcgctt tccttcacga ccaggcgaaa gcggcgcgtc
    14641 tggcgggtca gtaacgtctg accggtggtg gtcagcgccg cccggatacg ccgcgtatct
    14701 tctgccaccc ggcaactgac actgcgtacc gcatcgcgca ccgccgcaag tacagccctg
    14761 atttcgtctt cggtcagcca ttcaccggca tacggaaaag acacctgcgt tccgtctgcc
    14821 ggaagcaccg gcagcgactg ccactgactg ccggacagaa caagttccgc tccggttccg
    14881 gtcagtttct gaatggcgga ggccaggtgc ggcggcagta cgccgccgtg gcggttaacg
    14941 agtaacgtcg ccagccgggg cgcatccagc agaacataaa tgctgatcca gcccggatac
    15001 ttcgggttac gctggtcgac atccgtctct tccggccagc cctgcgccag cagcaggtta
    15061 cgggcgtgac gacaaatcag tgcgttcagc gttgtgtttt cgttcatgat taatcgttct
    15121 ccctgtatcg ccgcccgcag gcggcgataa catcagccgt tttcgtgtgt gttgtttttc
    15181 agcaggatgc gggcctgatt aaccaccgcc tgacggcggg taacgcattc cgggacggcg
    15241 agaccgtaat cacgggcggc ccattcacgt ttccacagtg cctgttcggc ggtctgaatc
    15301 agggacgcca gcagttcagc cgggacggtc acctggcggt tttcgctcac gttgctgata
    15361 atctccatcg ccgcggcaga agaaataccg gactcaattc gctggtcttc cgggatgtcc
    15421 tccaggcgcg ccaggacggt cctgatttca ggatcggaca gggaaatatc cattccgtca
    15481 gcgagggcct gaatctcttc cggggaccag ataaccagca tcagcggtgt atatcccgga
    15541 tactttgctg ccagctcccg gcatagcgtt tcgcatgttc cgtacattct cgtctccttc
    15601 tcaatcttta aagcacgcat ccagttccag aatttcggta tacgcctcca tgaacaggcg
    15661 attggcgcga tacagcgtct ggcgtgccag gcgttcaaca agcacatcgg aatcaaagcg
    15721 acagcgatgc tccatatcgt tcagcctggc ccgaaggtca cacatggtgt tggccacctc
    15781 cgccacgtcg gcgcgaagtt tttcatgtcc ggcatagttc atttacgcag cctccggcat
    15841 aaaccagtca tcatttgcgg ccccctgctg catggcccgc tgtacggcag ccagacgttg
    15901 ctgtccggca cggtgatact gttcaagcag ctcgataccg atataccggc gtccggactg
    15961 aagggcggcc acacaggttg agccgctgcc tgcaaacggg tccagcacaa tcgcgttcgg
    16021 gtgtgtgaag ctctcaatca gtggttgcag gctggtaaca ggcttttccg tcgggtgatg
    16081 acgattgcct gaatatttcc agcccagcac gtcgggcaat ggcttctgag gcagcgccgg
    16141 acgccctttt gccaggatgt aagcgcattc gtggcgatag cccacatatg cggccttcga
    16201 agtgtaggtt ttggtaaaca ccaggtggcc aaccacgctg aatcccgcat ttttccaggc
    16261 ggccataaag cgatcgacgc ggttccagcc atagaagctc accatcagcg catctttttt
    16321 cagcacgcgg tacatttcat tacaggccgg ttgcagccat tcatcggttt tatcgccggc
    16381 gatggtacgc ccctgacggt cacggaaacc gacgagatat ggcgggtcgg tgaggatgaa
    16441 atcgacacca ttgcccggaa tcgtggccat aacacgcacg cagtcaccct ggataaaacg
    16501 ggacataaca gcactccttc tcttacagag ggcaccggga gggcgcccct gtggcgcccg
    16561 ttccccgcgg cctgtgggct gttcaccgcg tccggtaacg ggtcaggccg accgtggaat
    16621 atcgcagccg tcgtcttttt gacggcgaga atatgccgcg aaagcggctt gaagggcgtt
    16681 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga tgccagccgg gagcggtaaa
    16741 agcgcccgtc ccgtgcccgg ccggacgcgg gacactgacc acaggcagcg ttcctgccgc
    16801 gccgcgtcag ccggcgcagt aaaaagagga gcccggcgac atcctttacc ggaaaagaaa
    16861 cgggcggttg cccgcccgtc tccgtcatac ttcatccacc gtaatgcgcc accttgtcga
    16921 ctatccactc cggcgcggtg acttccggca gctcatcatc atctgtcggg gaagaccatt
    16981 ccagttcccc ccacagcaca tcaacaagct ctccacgctc atatcgctgc cagccacaga
    17041 aattgcagcc ctgcccggcg taccagtgct ccagcatgca actgaaacgg cggcttaata
    17101 ctgcaataac gtcgctttcc ggctgacacc acggcgtgtc gaaatcaacc tgaatgaagt
    17161 tgtctccctg actgctgata ttcacctcat acccacaagg ccacttcacg ccatactgtt
    17221 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccgtta aagccattca
    17281 cttcaacatc caggcgagtg gacagaaccg tcagcaggtc aaacggcatg gttttttccg
    17341 gcaggacgtt gtcacacaga cggttccacc acacagatac atcctcgtta ctccagatgt
    17401 cgcaccagtc acctcttttt ggggtgaaaa gagcggtaat gctttccctg gcatcatccg
    17461 gtaattcttc ccagcgacgc cggccagtac cgctctgcag ccagagttca tgcagcatca
    17521 ggcagttttg ctcatccaga agtacaccgt cctgcaggtg tgtcagccag cgggtgaacg
    17581 cgatattctc cggcgataca acgccacgcc cggcagccgt cagtccgggg catggttcaa
    17641 accgcacatc ttcagtggtc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
    17701 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
    17761 cagcgatctg tgccggttca ccagaaaaat acatacgatc gggtagcagt gatgcattac
    17821 tgcatcaccg ccccctaaga accgtacgtg ccagtttccc ggcatacggc tcaagccttt
    17881 ctaagcccac tctcatgagc cggtttcact acatgtatac ctttcgcatg aacaagtgtg
    17941 tgacacatcg gatgcagcat aatcaaattg gagaggcagt tgctccctcc atcgactcgc
    18001 cgtatgatgt gatgaacctg ccactgggaa tcagatgtaa tcctctcatc acatatgggg
    18061 catcggcccc gctgctcaaa ccagatttga atgagctttt tccgtccttg cagtttgtgc
    18121 cgcattttgt ctgcacaacg ctcctcaaag tacatctccc attgaggatc gaacggatta
    18181 gctgcaattt taatcggttt aaaccggttg ataggcgtat cgattgcttt cctcagattt
    18241 gcatactgag gcttaccgtt ggcaaggagt tctccggtag ccgtggagaa agaccagttt
    18301 cgggcacctt gttgatggaa atatcttttc cgaatccact tctttccctt attgggatga
    18361 cgccgaacag cccaacgcca taaagccagc cagatctcat tgtcaacttt gttaaatgcg
    18421 accttggcca caatgtgttg atggtaagcc gcccaccctt tgatcattgg gttcagcatg
    18481 cggatcaacg taagctgatc catagctttg ttgcctttga ttacagcgcg tattttcttt
    18541 aaaaaggaac tgacattggc tttagagggt ttgatcagca tcttaccgcc atatttgcga
    18601 atgttctgtc caagaaaatc aaatccttcc tcgatatgtg tgattttcgt cttttccgga
    18661 gaaagcatta atcctctttc agccataaac cgcctgacga cgggtaatac ctgagactca
    18721 agaagttctt ttgactctcc agtgataata aaatcatcag catatctcac atagttaact
    18781 ttcgggttaa ccaatcgtcc cttgactgtt ctctttttaa actccgaagc aagaagcgcc
    18841 tccagtccat ccagagcaat gttagccagt acaggagaaa ttattccccc ctgaggagtt
    18901 cctgaattta cagggaataa ctgccccttt tccctgtaac ccgctttaag ccactttgtc
    18961 aggatttgtc tgtctgttga ggtgttatcg attagccatt gatgagatat attgtcaaag
    19021 catccccgta tatccccctc aagcacccat tcagcggatg ttttctgcgc aagaacacag
    19081 aagcactgtg ctatcgcatc tgcagtagac cttccagtcc gaaagccgaa ggatctctgg
    19141 tcagcagtga cttcagcaac gggctcaaga gccagcaaat acaacgcctg catcgcccga
    19201 tccttcatag tgggtatccc aaggggacga gacttcccat ttgacttagg tatataaacc
    19261 cgcttaagtg gctgtggttt gtaaccacgc cgtttcagca ggttaatagc aagaaattta
    19321 acctccgggg aagaccatgt ctgaccatca accccaggag tacgtttccc gctattctct
    19381 gtaactcgtc tgacagcaac tgctctgcca ctaaacgagt gagtcagtat ccattgcaag
    19441 gatttgacct tgccatgttt accttctcgt gttgccttta cgatacgcgc ttgcagtctc
    19501 cgcacttccc gatagcactg acgccagttg atactgtgcc aggagtcacc gttaagtaag
    19561 gacgcacctt tacaggttgt agcctgctca gtcatctgct tttcctttct ggaagattac
    19621 tcaaactctc tcgtaatgaa agacccagtg gaagtctgcc cgctttcgcg tagggtaatg
    19681 ttgccgggct caacccttat ccggtccatt acagactggc attcgctttt tccaccttct
    19741 tttacccgca ccgccaacag tatcccttgc ggttaacctg cctcacgagg cagcgatacg
    19801 ggcttaccat gttccacctg aataacaaca gtaggttaga ctctgctcac aacaccggtg
    19861 gtttattgtc cgtgtacccc tagcaaacag aggagtaacc aaccacatac cttttggttc
    19921 aagcctgtca gcatctttgg cttgttctgg ttaacgacgc ttcagcagtt cacatatgtt
    19981 agtcatgcta ccgagcctag acctcctccg cctgatgctg gcagattaca tgtaacctca
    20041 cggtttcatg cgatccgaag atccggtttc attgtcctga cagcttcaca ccccaccgtt
    20101 accagtgacg catgtgtcag taggctactg atgacggaac atcaggttcc ctgacggaat
    20161 cgaactccgc tggaacaatt attcaggcga cttcatgtcg cacattcgag caccagtttg
    20221 gcatgggaat tctcctctca catgcggcct ccatcaggcc gtgccgcacc tgtaaccggg
    20281 cgaccgcata cgaatgcagg tgcggccccg aagccgcagc gcgtggagga ggaacgacga
    20341 aacgtgaccg ggtgaggagg ccggcgacag cgacacactt gcatcggatg cagcccggtt
    20401 aacgtgccgg cacggcctgg gtaaccaggt attttgtcca cataaccgtg cgcaaaatgt
    20461 tgtggataag caggacacag cagcaatcca cagcaggcat acaaccgcac accgaggtta
    20521 ctccgttcta caggttacga cgacatgtca atacttgccc ttgacaggca ttgatagaat
    20581 cgtagtctca cgctgatagt ctgatcgaca atacaagtgg gaccgtggtc ccagaccgat
    20641 aatcagaccg acaacacgag tgggaccgtg gtcccagacc gataatcaga ccgacaacac
    20701 gagtgggacc gtggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc
    20761 cagtctgatt atcagaccga cgatacaagt ggaacagtgg gcccagagag aatattcagg
    20821 ccagttatgc tttctggcct gtaacaaagg acattaagta aagacagata aacgtagact
    20881 aaaacgtggt cgcatcaggg tgctggcttt tcaagttcct taagaatggc ctcaattttc
    20941 tctatacact cagttggaac acgagacctg tccaggttaa gcaccatttt atcgccctta
    21001 tacaatactg tcgctccagg agcaaactga tgtcgtgagc ttaaactagt tcttgatgca
    21061 gatgacgttt taagcacaga agttaaaaga gtgataactt cttcagcttc aaatatcacc
    21121 ccagcttttt tctgctcatg aaggttagat gcctgctgct taagtaattc ctctttatct
    21181 gtaaaggctt tttgaagtgc atcacctgac cgggcagata gttcaccggg gtgagaaaaa
    21241 agagcaacaa ctgatttagg caatttggct gtgttgatac agcgggtaat aatcttacgt
    21301 gaaatatttt ccgcatcagc cagcgcagaa atatttccag caaattcatt ctgcaatcgg
    21361 cttgcataac gctgaccacg ttcataagca cttgttgggc gataatcgtt acccaatctg
    21421 gataatgcag ccatctgctc atcatccagc tcgccaacca gaacacgata atcactttcg
    21481 gtaagtgcag cagctttacg acggcgactc ccatcggcaa tttctatgac accagatact
    21541 cttcgaccga acgccggtgt ctgttgacca gtcagtagaa aagaagggat gagatcatcc
    21601 agtgcgtcct cagtaagcag ctcctggtca cgttcattac ctgaccatac ccgagaggtc
    21661 ttctcaacac tatcaccccg gagcacttca agagtaaact tcacatcccg accacataca
    21721 ggcaaagtaa tggcattacc gcgagccatt actcctacgc gcgcaattaa cgaatccacc
    21781 atcggggcag ctggtgtcga taacgaagta tcttcaaccg gttgagtatt gagcgtatgt
    21841 tttggaataa caggcgcacg cttcattatc taatctccca gcgtggttta atcagacgat
    21901 cgaaaatttc attgcagaca ggttcccaaa tagaaagagc atttctccag gcaccagttg
    21961 aagagcgttg atcaatggcc tgttcaaaaa cagttctcat ccggatctga cctttaccaa
    22021 cttcatccgt ttcacgtaca acatttttta gaaccatgct tccccaggca tcccgaattt
    22081 gctcctccat ccacggggac tgagagccat tactattgct gtatttggta agcaaaatac
    22141 gtacatcagg ctcgaaccct ttaagatcaa cgttcttgag cagatcacga agcatatcga
    22201 aaaactgcag tgcggaggtg tagtcaaaca actcagcagg agtgggaaca atcagcacat
    22261 cagcagcaca tacgacatta atcgtgccga tacccaggtt aggcgcgctg tcaataacta
    22321 tgacatcata gtcatgagca acagtttcaa tggccagtcg gagcatcagg tgtggatcgg
    22381 tgggcagttt accttcatca aatttgccca ttaactcagt ttcaatacgg tgcagagcca
    22441 gacaggaagg aataatgtca agccccggcc agcaagtggg ctttattgca taagtgacat
    22501 cgtccttttc cccaagatag aaaggcagga gagtgtcttc tgcatgaata tgaagatctg
    22561 gtacccatcc gtgatacatt gaggctgttc cctgggggtc gttaccttcc acgagcaaaa
    22621 cacgtagccc cttcagagcc agatcctgag caagatgaac agaaactgag gttttgtaaa
    22681 cgccaccttt atgggcagca accccgatca ccggtggaaa tacgtcttca gcacgtcgca
    22741 atcgcgtacc aaacacatca cgcatatgat taatttgttc aattgtataa ccaacacgtt
    22801 gctcaacccg tcctcgaatt tccatatccg ggtgcggtag tcgccctgct ttctcggcat
    22861 ctctgatagc ctgagaagaa accccaacta aatccgctgc ttcacctatt ctccagcgcc
    22921 gggttatttt cctcgcttcc gggctgtcat cattaaactg tgcaatggcg atagccttcg
    22981 tcatttcatg accagcgttt atgcactggt taagtgtttc catgagtttc attctgaaca
    23041 tcctttaatc attgctttgc atttttttat taaatcttgc aatttactgc aaagcaacaa
    23101 caaaatcgca aagtcatcaa aaaaccgcaa agttgtttaa aataagagca acactacaaa
    23161 aggagataag aagagcacat acctcagtca cttattatca ctagcgctcg ccgcagccgt
    23221 gtaaccgagc atagcgagcg aactggcgag gaagcaaaga agaactgttc tgtcagatag
    23281 ctcttacgct cagcgcaaga agaaatatcc accgtgggaa aaactccagg tagaggtaca
    23341 cacgcggata gccaattcag agtaataaac tgtgataatc aaccctcatc aatgatgacg
    23401 aactaacccc cgatatcagg tcacatgacg aagggaaaga gaaggaaatc aactgtgaca
    23461 aactgccctc aaatttggct tccttaaaaa ttacagttca aaaagtatga gaaaatccat
    23521 gcaggctgaa ggaaacagca aaactgtgac aaattaccct cagtaggtca gaacaaatgt
    23581 gacgaaccac cctcaaatct gtgacagata accctcagac tatcctgtcg tcatggaagt
    23641 gatatcgcgg aaggaaaata cgatatgagt cgtctggcgg cctttctttt tctcaatgta
    23701 tgagaggcgc attggagttc tgctgttgat ctcattaaca cagacctgca ggaagcggcg
    23761 gcggaagtca ggcatacgct ggtaactttg aggcagctgg taacgctcta tgatccagtc
    23821 aattttcaga gagacgatgc ctgagccatc cggcttacgg t
//
LOCUS       DAZKIF010000075        23848 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZKIF010000075 DAZKIF010000000
VERSION     DAZKIF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.075"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(25..3906)
                     /gene="entF"
                     /locus_tag="ACHOTW_002865"
     CDS             complement(25..3906)
                     /gene="entF"
                     /locus_tag="ACHOTW_002865"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP9844932.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(3903..4121)
                     /locus_tag="ACHOTW_002866"
     CDS             complement(3903..4121)
                     /locus_tag="ACHOTW_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HFP9844933.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4124..5326)
                     /gene="fes"
                     /locus_tag="ACHOTW_002867"
     CDS             complement(4124..5326)
                     /gene="fes"
                     /locus_tag="ACHOTW_002867"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP9844934.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5569..7809
                     /gene="fepA"
                     /locus_tag="ACHOTW_002868"
     CDS             5569..7809
                     /gene="fepA"
                     /locus_tag="ACHOTW_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFP9844935.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7984..8604
                     /gene="entD"
                     /locus_tag="ACHOTW_002869"
     CDS             7984..8604
                     /gene="entD"
                     /locus_tag="ACHOTW_002869"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP9844936.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8726..8878)
                     /gene="hokE"
                     /locus_tag="ACHOTW_002870"
     CDS             complement(8726..8878)
                     /gene="hokE"
                     /locus_tag="ACHOTW_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP9844937.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9229..9381)
                     /gene="hokE"
                     /locus_tag="ACHOTW_002871"
     CDS             complement(9229..9381)
                     /gene="hokE"
                     /locus_tag="ACHOTW_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP9844938.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            9350..9617
                     /locus_tag="ACHOTW_002872"
                     /pseudo
     CDS             9350..9617
                     /locus_tag="ACHOTW_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9820..10938
                     /locus_tag="ACHOTW_002873"
     CDS             9820..10938
                     /locus_tag="ACHOTW_002873"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP9844939.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            11004..11252
                     /locus_tag="ACHOTW_002874"
     CDS             11004..11252
                     /locus_tag="ACHOTW_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP9844940.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            11317..11685
                     /locus_tag="ACHOTW_002875"
     CDS             11317..11685
                     /locus_tag="ACHOTW_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9844941.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11779..12432
                     /gene="nfsB"
                     /locus_tag="ACHOTW_002876"
     CDS             11779..12432
                     /gene="nfsB"
                     /locus_tag="ACHOTW_002876"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP9844942.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12540..13787
                     /gene="ybdG"
                     /locus_tag="ACHOTW_002877"
     CDS             12540..13787
                     /gene="ybdG"
                     /locus_tag="ACHOTW_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP9844943.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13855..15231)
                     /gene="pheP"
                     /locus_tag="ACHOTW_002878"
     CDS             complement(13855..15231)
                     /gene="pheP"
                     /locus_tag="ACHOTW_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP9844944.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15333..18470)
                     /gene="cusA"
                     /locus_tag="ACHOTW_002879"
     CDS             complement(15333..18470)
                     /gene="cusA"
                     /locus_tag="ACHOTW_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP9844945.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(18482..19705)
                     /gene="cusB"
                     /locus_tag="ACHOTW_002880"
     CDS             complement(18482..19705)
                     /gene="cusB"
                     /locus_tag="ACHOTW_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP9844946.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19721..20053)
                     /gene="cusF"
                     /locus_tag="ACHOTW_002881"
     CDS             complement(19721..20053)
                     /gene="cusF"
                     /locus_tag="ACHOTW_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP9844947.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20077..21459)
                     /gene="cusC"
                     /locus_tag="ACHOTW_002882"
     CDS             complement(20077..21459)
                     /gene="cusC"
                     /locus_tag="ACHOTW_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP9844948.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21616..22299
                     /gene="cusR"
                     /locus_tag="ACHOTW_002883"
     CDS             21616..22299
                     /gene="cusR"
                     /locus_tag="ACHOTW_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP9844949.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22289..23737
                     /gene="cusS"
                     /locus_tag="ACHOTW_002884"
     CDS             22289..23737
                     /gene="cusS"
                     /locus_tag="ACHOTW_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP9844950.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
BASE COUNT         5735 a         6506 c         6056 g         5551 t
ORIGIN      
        1 tatgggttta tagataatgt taatttatct gtttagcgtt gcgcgaataa tcggcccaat
       61 tttttcaaat gcccctggag agataatatc cacatgcgca caatcctgac gatagatatc
      121 cagctccgca atccacggcg accaggcgcg ttcggggctc ataccttcct gaagcgtacg
      181 ttcagcaaca aacagcgtcg cttttccgtc aaacggtacg ctatgagcag tcgtcagcag
      241 gcgcacagca tcagcgtagt tgccttcaat ggtggtaaac aactccgttg aagtacttcc
      301 ctgctgtgcc gccaggaagg cctcgcgctc gcggttaatc tccgccagca cttccgggtc
      361 cagaccatta gcttcttttt cctgccagtt ttgcgtttct ggcggccagg tatccagcaa
      421 gccaagaaat gccacctgtt cgccacgggc acgtagtcgc gccgcaatgc cctgcgccag
      481 tgtaccgcca agggaatacc ccagcaggta ataagggccg tgcggttgtt gttcaagtaa
      541 cgttgccaga tgcgcttcgc agacttcatc caggttcgtc gctgtctgca tggggccatg
      601 agggcgcggc gactgaatgc cgataatcga ccatagtgga tcgagataac gcgagagcac
      661 gctaaactgc caggcaaaac cggatgccgg atggaaacaa aacagcgtcg ggccattacc
      721 ttcacgcaac ggcagaatgg tttcgaatcc catgcgccgg gagctgtcct cttcaccatc
      781 aataatcgtt gccagtttgg cgacggttga cgcgaccatc acctgccccg gcgtcacctg
      841 acgggcaaac tgccgactta actgcgctgc cagtttcatt gccagtagcg aatgaccgcc
      901 aagcgcgaag aaatcagcat cggcatcctg cacgtcacaa cccagcaacg acgcgaatgc
      961 cgcagcgata atcgtttcac tgcccgcttt cggcgcacgc cccggcgttt gtgccttaag
     1021 ttcaggcaac ggtaaggctt tgcgatccag cttgccgttg gcgctaagag gtaactgtgg
     1081 aagttgcagc agaacgaccg gcgccatatg cggcggcaat gtttcgcgaa gctgtgcctg
     1141 taatgcgctg gtatccaacg gcagacctga ttgcgacacc agatagccca ccaactgacg
     1201 cgcatcacca ccggtggctg ccgcctggtt aatcacacag gcgtgggtaa cggcttgttc
     1261 gacatccggc agcgcctgca tcacgcgatc gatttcgccc agttcgatac gctgcccgcg
     1321 aatttttagc tgatcgtcac tgcgcccgag gtactccacc gcgccgttat ccagccagcg
     1381 ggcaacgtct ccggtacggt acatccgttc accagggaca aaaggatcgg caatgaagcg
     1441 gctggcggtc agatcgggtc gtccaagata cccctgcgcc agttgaatac cggtgagata
     1501 gagatctccc gccacacccg gcggcaccgg atgcatcatc gcatcgagaa tgcgcaagcc
     1561 cgtattccac accggataac caatcggcac actgctgccg cgcacctgtg ccagttcctc
     1621 gccaaaagcc ggataccaac tcacatctac cgccgcttcc gtcgggccat atagattatg
     1681 caacggcgcg cccgttaact gttgccattc gcggcataaa tcagccggta aggcctcacc
     1741 actacagaaa acctgtttca acgtcgcgca attctggcga gcggtttgcg gcgtcagcga
     1801 tgcaacaaat gccgccagca tcgacggcac aaagtgcgtg gtcgttacgc catattcggc
     1861 aaagaattgc tgcatagcga gcgggtcgcg gtgcgcttcc ggttcagcca tcaccagttt
     1921 agccccggca ataaacggcc agaaaaactc ccacaccgag acatcaaaac tgcacggcgt
     1981 tttttgggca acgacatctt cacctgtaag tggataatga ttttgcatcc acaacaggcg
     2041 gttaacgata gccgtctgcc cgaccattac ccctttcggc ctgccggtgg agccagaggt
     2101 aaagatgata taagccgtgt gatgcggttg tgaaagttgc agcggcgcac tgccctgcgg
     2161 tgtaagcggg gcgttatagc aaaggttggt taaatctgga acatcggcaa agcgcggcag
     2221 ttgatcgtcg gtggtgatta acagcgacgg acgcgcatct tccagcatca ttttcaggcg
     2281 atcgtccgga taaccggtat ccagcggtaa ccaggccgca cctgcttcaa caatcgcatg
     2341 tagcgccagg gtcaaaaaga ccgagcgcgg taatgccacc gccacgctgt cccctggttt
     2401 aacgccgtgc tcacgcagca gattcgccag cgccaccacc tgctcgcgca tttcccgata
     2461 gctgaactgg taacgcgcat ctgccagcgc cggagcatcc ggtgtttttg ccgcttgttc
     2521 tgccaccagc gcgctaagcg tggtttctgg aatctcaacc tgagtggcgt tgatctgcgc
     2581 cagctgcgca tactcacctg gcagcataat atcaacatcg ccgcacaaca gagccggatc
     2641 cgcagcgaat tgggcgatca gcattttcag gcgttcagca tgctggatta acgttggctc
     2701 atcgtaatgc tgtttattgg cgaggatctc aatactcaaa tcaccgtgtt catccgggaa
     2761 cagggccagt tcaaggtcat taaccggacc ggttgccagg gtatgggttt gcgcctgaac
     2821 accaggaata tccagttggt aatcaaatac cttgatattg agtaccggac caaacagcgg
     2881 ttcatcacct gccgctcgcc cgctgtcacg gacaatttgt tcggcatcgt aacgttgatg
     2941 acgacgcatt tttttcagtt gtgctgccag tcgggttgcc agttccggca gcgtttcttg
     3001 tgccgcaatg tgaataccca acggtaaaac gttgagcacg ggtccggtag ccgtcagcgc
     3061 cgccgagccc agtcgacgca taaagataaa tccggcggcg tagtccatgc gattgcacaa
     3121 tcgccccagc caaaaggctg ccagcgcaag ggctaaatcg gtacgctgca cacctgaaag
     3181 ttgcgtagcc agctggcgga attccccgtc ggtaaattcc agtttcaggc gtagaatatc
     3241 tgccgaagcg ctgcgccccg ctaaaggtgc cggagaaagt gacgcgggcg gcggcagttg
     3301 acgacgctgt tccgcccaga atgccgcatc acgctgccag gcttcgcttt cgcggtattg
     3361 ctggtactct tccactacat cagcgaaagg cgtaaacggc gaagcaggcg tttgttcgcc
     3421 acgcagcaat gcgcagtaaa tgttggcgat ctgccgagta atggccggga aactgaagcc
     3481 atcgaccagc aaatggtgat aacgctgata ccagtaccag cggttatctg ccacctgtat
     3541 cagctggtga aagaccagtg gtttaccgct atcgacgcgc aaatcttgtt gcaaatccgc
     3601 ctgcattaat gcctgcgcag taccgtgcgg atcaatattg gttcgcaggt cgataatttc
     3661 tggcagttcg aatatcagcg catcatcgac ccattgccag acttcgccgt tatcttccgt
     3721 aaaacgcatc cgcagcgtat ctgcttgcgc taatccggca accaccgcgc gggccagtaa
     3781 tggcgcatca acctctccgg ttaactcaac gtaatgcgcc acgctccagg cggagggtaa
     3841 ttctgacagt ttttctgcca tccagatgcc gggctgtgcg gcgaccaaag gtaaatgctg
     3901 gctcattgtg cctcctgcaa ctgggtgaaa ttcgccggtg tcagagtacg ccagtggact
     3961 tccagccact gctggcagga ctcttgtgac tgcggctgac acacaatgtc ccagcctgcc
     4021 ggtaaggcgc attgttgcgg ccacagactg aattgcccct gcgcattgcg caaaatgtaa
     4081 aacactccct gtggatcatc gaagggatta ctgaatgcca tattcaactc ctgtcatgga
     4141 aaagtggttg ccagaggtcg attaacccct gcatcaagcc accgcgccaa caaagcgcat
     4201 catgtccgcc gtcaacctga cgccagaaaa tggattcttt tatagggtgt aattgcgcat
     4261 acagcgcctg attggcccgc atgatcatcg gctcgcgaat acccgcttcc agcacaatgc
     4321 gcagaccttc ggcgctaact tcaccagctt ttagcttttc aagtaacacg ccctcttgct
     4381 gcccgccccg atgcggccac cagtacgatc ctgactggct taatacacag ccaaagcgtt
     4441 caggccagtg cagtccggca tacagcgcgg acagcccacc aaaactctgc ccggcgacca
     4501 cggtgcgatc ggcgcgatcg ctaaaagggg caatagcttt caccaggggt aataactctt
     4561 gctgtactgc cagccagaaa tccgcattac acggcagttc gtgggcgcgg tgcgtggtgt
     4621 cgatagcgtc gatcaacaca tacacggcgg gaggaagttg ctgacgatgg gtcagcgaaa
     4681 tcagcacagg ccagacgggc atactttgcg cccaaaattc gccatcgagc aaaactgcca
     4741 gcgggcgttc ttctgctgtt acatcgccgg tggtaaaaat ccatacacga cgtgaatttt
     4801 tcaaccgttc acttttccag ataatttctt tggctggtat ttctggcgct tgcggacaat
     4861 cccatcccgg ttgcagaggc gcttgcggca tttcgagtgc agaaacagcg tgccctcgcc
     4921 cgcctttcca gctttgtgga ttcagcggat cggctattgc ctggggtaat agttttcgcc
     4981 agccttcacg taattcgagg cgatcggggg atggtgcaga aaaaatgtca tcgcgttcgg
     5041 tgggaataaa gcagtagctg ccgcgccagt tggcattgag ttgtgtcgtc cactgccaga
     5101 catcagtacc tgcaattcgc tgcatcgact ggggctggct gttctgatga tgatcggtca
     5161 caccagtgat gtagatccat acgcgcttta tcgtcgagta ttcttcagaa ccttggggat
     5221 cacgccacca gaaagtgacc tcaaacattt cgtcattcag acgctgccat tccgggccat
     5281 gtttcgactg ccaccagctc tcacttccta cttttaacgc cgtcacacca taaccccatg
     5341 tttactgtgc aatttttcat tgattgcaga aatatattga taatattatt gataactatt
     5401 tgcatttgca atagcgtaat ggcgcgccgt gggaagcgcg gacattaatt aaccaactgc
     5461 actgcgtgtc tttcaggatc aaaggttttc gcggtagcgg gatgcgtcgt gttgatgacg
     5521 accatgcccg acagttgcaa ttcgtggcaa aaatgcagga ataaaacaat gaacaagaag
     5581 attcattccc tggccttgtt ggtcaatctg gggatttatg gggtagcgca ggcacaagag
     5641 ccgaccgata ctcctgtttc acatgacgat actattgtcg ttaccgccgc cgagcagaac
     5701 ttacaggcgc ctggcgtttc gaccatcacc gcagatgaaa tccgcaaaaa ccccgttgcc
     5761 cgcgatgttt cggagatcat ccgtaccatg ccaggcgtta acctgaccgg taactccacc
     5821 agtggtcagc gagggaataa ccgacagatt gatattcgcg gtatgggtcc ggaaaacacg
     5881 ctgattttga ttgacggcaa gccggtaagc agccgtaact cggtgcgtca gggctggcgt
     5941 ggcgagcgcg atacccgtgg tgatacctcc tgggtgccac ctgaaatgat tgaacgtatt
     6001 gaagttctgc gtggtccggc agctgcgcgt tatggcaacg gcgcggcggg cggcgtggtt
     6061 aacatcatta ccaaaaaagg cagcggcgag tggcacggct cctgggacgc atatttcaat
     6121 gcgccagaac ataaagagga aggtgccacc aaacgcacta actttagcct gaccggtccg
     6181 ctgggcgacg aattcagctt ccgcttgtat ggcaacctcg acaaaaccca ggctgacgcg
     6241 tgggatatca accagggtca tcagtccgcg cgtgccggaa cgtatgccac gacgttacca
     6301 gccgggcgcg aaggggtgat caataaagat attaatggcg tggtgcgctg ggacttcgca
     6361 ccattgcaat cgctggaact ggaagcgggt tacagccgcc agggtaacct gtatgcgggc
     6421 gatacccaga acaccaactc tgacgcttac actcgctcga aatatggcga tgaaaccaac
     6481 cgcctgtatc gccagaacta ctcgctgacc tggaacggtg gctgggataa cggcgtgacc
     6541 accagcaact gggtgcagta cgaacacacc cgtaactcgc gtattccgga aggtctggcg
     6601 ggcggtaccg aagggaaatt taacgaaaaa gcgacacagg atttcgtcga taacgatctt
     6661 gatgacgtga tgctgcatag cgaagttaac ctgccgattg atttcctcgt taaccagacg
     6721 ctgacgctgg gtacggagtg gaatcagcag cggatgaagg acttaagttc caacacccag
     6781 gcactgaccg gaacgaatac cggtggcgct attgatggcg tgagtgccac cgaccgtagc
     6841 ccgtattcaa aagcagaaat tttctcgctg tttgccgaaa acaacatgga gctgactgac
     6901 agcaccatcg taacgccggg gctgcgtttc gatcatcaca gtattgtcgg caataactgg
     6961 agcccggcgc tgaacatatc gcaaggttta ggcgatgact tcacgctgaa aatgggcatt
     7021 gcccgcgcct ataaagcgcc gagcctgtac cagactaacc cgaactacat tctctacagt
     7081 aaaggtcagg gctgctatgc cagcgcgggc ggctgctatc tgcaaggtaa cgatgacctg
     7141 aaagcagaaa ccagcatcaa caaagagatt ggtctggagt tcaaacgcga cggctggctg
     7201 gcgggcatca cctggttccg taacgattat cgcaataaga ttgaagcagg ctatgttgct
     7261 gtagggcaaa acgcagtcgg caccgatctc tatcagtggg ataacgtgcc gaaagcggtg
     7321 gttgaaggtc tggaaggatc gttaaacgta ccggttagcg aaacggtgat gtggactaat
     7381 aacatcactt atatgctgaa gagtgaaaac aaaaccacgg gcgaccgttt gtcgatcatc
     7441 ccggagtata cgttgaactc aacgctgagc tggcaggcac gagaagattt gtcgatgcaa
     7501 acgaccttca cctggtacgg taagcagcag ccgaagaagt acaactataa aggtcagcca
     7561 gcggttggac cggaaaccaa agaaattagt ccttacagca ttgttgggct gagcgcgacg
     7621 tgggatgtga cgaagaatgt cagtctgacc ggcggcgtgg acaatctgtt cgacaaacgt
     7681 ttgtggcgtg cgggtaatgc ccagaccacg ggcgatttgg caggggccaa ctatatcgcc
     7741 ggtgccgggg cgtataccta taacgagccg ggacgtacgt ggtatatgag cgtaaacact
     7801 cacttctgat gctaacgtca gattgttgac aaagtgcgcg tcgttcatgc cggatgcggc
     7861 gtgaacgcct tatccggcct acaaaaatct tgccaattca atatattgca ggaccatgta
     7921 ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgt catcagtctc gaatatggtc
     7981 gatatgaaaa ctacgcatac ctccctcccc tttgccggac atacgctgca ttttgttgag
     8041 ttcgatccgg cgaatttttg tgagcaggat ttactctggc tgccgcacta cgcacaactg
     8101 caacacgctg gacgtaaacg taaaacagag catttagccg gacggatcgc tgctgtttat
     8161 gccttgcggg aatatggcta taaatgtgtg cccgcaatcg gcgagctacg ccaaccggtg
     8221 tggcctgcgg aggtatacgg cagtattagc cactgcgggg ctacggcatt ggccgtggtt
     8281 tcccgtcaac cgattggcgt tgatatcgaa gaaatttttt ctgcgcaaac agcgaccgaa
     8341 ttgacagaca acattattac accagcagaa cacgagcgac tcgcagactg cggtttagcc
     8401 ttttctctgg cgctgacact ggcattttcc gccaaagaga gcgcatttaa ggcaagtgag
     8461 atccaaactg atgcaggttt tctggactat cagataatta gctggaataa acagcaggtc
     8521 atcattcatc gtgagaatga gatgtttgct gtgcactggc agataaaaga aaaaatagtc
     8581 ataactctgt gccaacacga ttaattgaca acatctggta cgattcgccc gcagccatca
     8641 ctgactgcgg gcgaaagtgt aaagcaggtg ccttaccact ctgacctgac aaccggatat
     8701 gcggggattg ctccccgcac aacggctact tcttcggttc gtaagcgaga acagccttaa
     8761 actcaatatt acgttccttt acggtgaact cacacagcga gtctccgacc agaagcgtaa
     8821 atcccagcac cgtcaaacac agcactatga ctgccacaag ggcatatttc gtcagcatgt
     8881 ttatattgcc tccatgcgaa gaagggacta ccatccgagt tgttggggct cggagtggca
     8941 gccccaggat tgatagaaat atctcctggg gcttcatctt tccagacctc agagttaacc
     9001 tgaaaccgga aagcttcagg cacccgccgt tatcttaccc ttccttagca aaatagctgt
     9061 ttatttatac agtgaatcct gtttcggact tctgtcggca aaactcatca ttgctcttaa
     9121 aacgaaatcg cccgcagcca tcactgaccg cgggcgaaag tgtaaagcag gtgccttacc
     9181 atcctgacct gacaaccgga tatgcgggga ttactccccg cacaacggct acttcttcgg
     9241 ttcgtaagcg agaacagccc tgaactcaat attacgttcc ttcaccgtaa actcacacag
     9301 cgagtctccg accagaagcg taaatcccgg taccgtcaaa cacagcacta tgactgccac
     9361 aagggcatat ttcgtcagca tgtttatatt gcctccatgc gaagaaggaa ctaccatcca
     9421 aattgtctgg tttggagtgg cagccccagg gttgatagaa atatctcctg gagcttcatc
     9481 tttccagacc tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc gttatcttac
     9541 ccttcctcaa caaaatagct gtttatttat acagtaaatc ctgtttcgga cttctcactc
     9601 ctcatttaat caactgaagc tgagcccgtt ttttctgcat gcctcaatcg tcaaaattct
     9661 tcgccaccag ccagccgtgc cataacttgt cacattgcaa aagtagccaa attgcttcag
     9721 tcaccatcac caaaaaaaga aacattccag aacttccgca tctcgcccag ccgctccgac
     9781 ttctatactg aatagaaaac tccaacataa gagaaaccta tgccattacc cgattttcat
     9841 gtttctgaac cttttaccct cggtattgaa ctggaaatgc aggtggttaa tccgccgggc
     9901 tatgacttaa gccaggactc ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc
     9961 ggagaggtaa agcacgatat caccgaaagt atgctggagc tggcgacgga tgtttgccgt
    10021 gatatcaacc aggctgccgg gcaattttca gcgatgcaga aagtcgtatt gcaggcagcc
    10081 gcagaccatc atctggaaat ttgcggcggt ggcacgcacc cgtttcagaa atggcagcgt
    10141 caggaggtat gcgacaacga acgctatcaa cgaacgctgg aaaactttgg ctatctcatc
    10201 cagcaggcga ccgtttttgg tcagcatgtc catgttggct gtgccagtgg cgatgacgcc
    10261 atttatttgc tgcacggctt gtcacggttt gtgccgcact ttatcgccct ttccgccgcg
    10321 tcgccatata tgcagggaac ggatacgcgt tttgcctcct cacgaccgaa tattttttcc
    10381 gcctttcctg ataatggccc gatgccgtgg gtcagtaact ggcaacaatt tgaagccctg
    10441 tttcgctgtc tgagttacac cacgatgatc gacagcatta aagatctgca ctgggatatt
    10501 cgccccagtc ctcattttgg cacggtggag gttcgggtga tggatacccc gttaaccctt
    10561 agccacgcgg taaatatggc gggattaatt caggccaccg cccactggtt actgacagaa
    10621 cgcccgttca aacataagga gaaagattac ctgctgtata aattcaaccg tttccaggcc
    10681 tgccgctatg ggctggaagg cgtcattacc gatccgtaca ctggcgatcg tcgaccacta
    10741 acggaagaca ccttgcgatt gctggaaaaa atcgcccctt ctgcacataa aattggtgca
    10801 tcgagcgcga ttgaggccct gcatcgccag gtcgtcagcg gtctgaatga agcgcagctg
    10861 atgcgcgatt tcgtcgccga tggcggctcg ctgattgggc tggtgaaaaa gcattgtgag
    10921 atctgggccg gtgactaagc cgggaattgc cttacagaag aatatccctg acaatggtgt
    10981 tttaacttac atttaacaat gttatgaaac accctttaga aaccttgacc accgcagcag
    11041 gcattttgct gatggctttc ctctcttgcc tgctgctgcc cgcccccgca ctggggctta
    11101 cgctggcaca aaaactggtg accacgttcc atctgatgga tcttagtcag ctttacactt
    11161 tattgttttg tctgtggttt ttagtgctgg gcgctattga gtattttgtt ctgcgcttta
    11221 tctggcgacg ctggttctcg ctggcggatt aaacgtcgat tatcgccgta cttcgcggca
    11281 tactttgctt attctccttc gcctaaagga acgtttatgg ataagcaatc actgcacgaa
    11341 acggcgaaac gcctggccct tgagttaccc tttgtcgagc tttgctggcc ttttggcccg
    11401 gagttcgatg tttttaaaat tggcggcaag atttttatgc tgtcgtcgga gctacgcggc
    11461 gtccccttta tcaatctgaa gtccgatcca caaaaatccc tgttaaatca gcaaatatac
    11521 ccaagcatta agccagggta tcacatgaat aaaaagcact ggatttcggt gtatcccggc
    11581 gaggaaatct ccgaagcgtt acttcgcgat ctgatcaacg attcgtggaa tctggtggtt
    11641 gatggtctgg ctaaacgcga tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag
    11701 cgcatttttc tcgcttacca tttctcgttg aaccttgtaa tctgctggca cgcaaaatta
    11761 ctttcacatg gagtctttat ggatatcatt tctgtcgcct taaagcgtca ttccactaag
    11821 gcatttgatg ccagcaaaaa acttaccccg gaacaggccg agcagatcaa aactctcctg
    11881 caatacagcc catccagcac caactcccag ccgtggcatt ttattgttgc cagcacggaa
    11941 gaaggtaaag cgcgtgttgc caaatccgct gccggtaatt atgtgttcaa cgaacgtaaa
    12001 atacttgatg cctcgcacgt cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg
    12061 ctgaagctgg ttgttgacca ggaagatgct gatggccgct ttgccacgcc ggaagcgaaa
    12121 gccgcgaacg ataaaggtcg caagttcttc gccgatatgc accgtaaaga tctgcatgat
    12181 gatgcagagt ggatggcaaa acaggtttat ctcaacgtcg gtaatttcct gctcggcgtg
    12241 gcggctctgg gtctggacgc ggtacccatc gaaggttttg acgccgccat cctcgatgca
    12301 gaatttggtc tgaaagagaa aggctacacc agtctggtgg tagttccggt gggtcatcac
    12361 agcgttgaag attttaacgc tacgctgccg aaatctcgtc tgccgcaaaa cattacctta
    12421 accgaagtgt aattctccct tgccgggcat ctgcccggct atttcccctc agattctcct
    12481 gatttgcata accctgtttc agccgtcatc ataggctgct gttgtataaa ggagacgtta
    12541 tgcaggattt aatatcccat gttgaagatt tagcgggtat tgagatcgat cacaccacct
    12601 cgatggtgat gattttcggt attatttttc tgaccgccgt cgtggtgcat attattttgc
    12661 attgggtggt actgcggacc ttcgaaaaac gtgccatcgc cagttcacgg ctttggttgc
    12721 aaatcattac ccagaataaa ctcttccacc gtttagcttt taccctgcag gggattatcg
    12781 tcaatattca ggcggtattc tggctgcaaa aaggcaccga agcggcagat attctgacta
    12841 cctgcgcgca gttgtggatc atgatgtatg cgctgctttc agtcttctcg ctgctggatg
    12901 ttattttgaa tctggcgcag aaattcccgg cagcatctca gttaccgctg aaagggatat
    12961 ttcaggggat taaactgatc ggcgcgattc tggtcggcat tttgatgatc tcgctgctga
    13021 ttggtcagtc gccagcgatt ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt
    13081 tggtatttaa agatccgatt cttggtctgg tggcaggtat tcagctttcc gcgaacgata
    13141 tgctgaaact gggcgactgg ctggagatgc cgaaatacgg cgcggatggc gcggtgatcg
    13201 atattgggtt aaccaccgtc aaggtgcgta actgggacaa taccattacc actattccca
    13261 cctggtctct ggtttctgac tcctttaaaa actggagcgg gatgtcagca tctggcgggc
    13321 gacgtattaa gcgcagtatc agtattgatg tcaccagtat tcgttttctt gatgaagacg
    13381 aaatgcaacg tctgaataaa gcgcatttgt taaagcctta tttaaccagc cgccatcagg
    13441 aaattaatga gtggaatcgc cagcaaggtt ctacggagtc ggtattaaac ctgcgccgaa
    13501 tgaccaatat tggaaccttt cgtgcctatc tgaacgaata tctgcgtaac catccgcgga
    13561 ttcgtaaaga tatgacctta atggtacgcc aactggctcc aggtgataac ggtttaccgc
    13621 tcgagatcta tgcgtttacc aacaccgtgg tgtggctgga atatgaaagc attcaggctg
    13681 atatattcga tcacatattt gcgattgtcg aagagtttgg tctgcgactt catcagtcgc
    13741 caaccggcaa tgatattcgc tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc
    13801 tctcatcgaa tggatgagag cggacatcgt tttgtcggat gtagcgtgaa tgccttattt
    13861 ccgacgcagc gttttaaatg ctacaaacag gaatacaatc cacaccggca gcaggatcgc
    13921 tgacaagcgc atatcatcca tcgtgcacat cagcagcaaa atcatggcga ggaaggcgat
    13981 gcaaagatag ttgccgaacg gatagagcag cgccttaaac tgtgtttcac gcccctgacg
    14041 tcgcatcgct gcacgaaaac gcagatgcgc cagacagatc ataatccagt tcaacagcag
    14101 cgttgctacc accagcgcca tcagcagacc aaacgctttt tgcggcagca gatagttgat
    14161 taacaccacc agcgaagtga tcgctccgga aagcatcagc gagttaatcg gcacgccgcg
    14221 acggctaaca cgggtaagga acttcggcgc attaccctgc acagaaaggc caaacagcat
    14281 gcggctgtta gagtaaaccc cgctgttata cactgacagc gatgctacca gaatgacgaa
    14341 gttcagcgca gaagctacca cgttgctgtc gagattatgg aaaatcatca caaacgggct
    14401 actgttggat ttcacttcca cccacggata gagcgccagt aaaaccacca gtgaaccgat
    14461 gtaaaacagc aggatgcgat acaccacctg atttaccgct tttggaatgc ttttttccgg
    14521 atcgcgcgct tcagcggcag taatcccaat cagctccaga ccgccgaagg agaacataat
    14581 taccgccagc gacaaaatca gcccattcca gccggtggcg aagaaaccac cgtagcgcca
    14641 gaggttgtcg atactggctt tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa
    14701 gccgatcata ccgatgattg ccagcacttt aatcagcgca aaccagaact cggtttcgcc
    14761 atataagcgc acgttcacca ggttaacggc gttgatgata ataaagaagg cggcagccca
    14821 aatccacgtt ggaacatccg ggaaccagta ctgcatatag atgcccgcag cggtcagctc
    14881 tgccattccc accagcacga acatcaccca gtagttccag ccggagagga acccggcgaa
    14941 cggtccccag tatttatagg caaagtgggc aaatgaaccg gataccggct cttcgaccac
    15001 catctcgccg agctggcgca taatcaggaa agcgatgatc ccggcgacgc cgtagcccag
    15061 caatacagcc ggacccgcca tctgaatcgc cgggccaatg ccaagaaaca gaccagtacc
    15121 aattgcgcca cccagcgcaa tcagttgaat atgacggtta tgtaatccgc gatgaagcgt
    15181 cggctcttga ttcgacgcag tatcttccga tacgtttgac gcgtttttca cgcctttccc
    15241 ctgtgtgtct tttttgttga ggggcacctt ttaacattta gtgcccatcg tcgcaagaca
    15301 caatccacac ggttaaaccg ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc
    15361 cacatcagct tatacgctgc cgggataata aacagcgaca gcaaaggcgc ggtgatcatg
    15421 ccgccaatca tcggcgcggc aatccggctc atcacctctg atcctgcccc cgttccccac
    15481 agaatcggca gcagaccagc gataatcacc gccaccgtca tcgctttcgg gcgcacgcgc
    15541 aggaccgcgc cgtgatgtaa cgcctcatcc agcttctgct cgctgaatgt ttgcggatta
    15601 ttcaacgacg gctcggcctc tatggcgtga cgtaaataca tcagcatcac cacgccaaat
    15661 tcggcggcga ccccggcaag ggcaataaaa ccggtgcccg tcgccacaga aagatgaaag
    15721 cccatccacc acagcaacca gatgccgccc accagcgcaa acggtacgct gctgataatc
    15781 agcaacgctt cgcccacccg acggaacgcc agatacaaca gcacgaagat gatcatcaac
    15841 gtcatcggca ccatgagttt cagcttatgg ttggcgcgct ccagtagctc gaactgcccg
    15901 gagaatgcca cgctggtgcc cggttttaac tgcacttttt cagctatcgc tttttgcaaa
    15961 tcgtgaacca ccgacaccat gtcacgatcg cgggcatcga tataaatcca gctcgtcggg
    16021 cgcgcattct cggttttcag catcgacggt ccggtagaga ctttaacgtc ggccacgtct
    16081 gccagggtga tttgctgctt catcggcgtc aggatcggca actggcgcag tgcctgcgga
    16141 ctatcgcgcc agctttgcgg ataacgcaga ttaattggat aacgggcaat cccttccacc
    16201 gtttcgccaa ccatcgcccc gcccaccgca gaagtcacaa acaactgcac atccgccacc
    16261 gtcataccgt aacgtgcggc tttttcacgg ttaatctcaa cgttgatata gcgcccacct
    16321 tccagccgct cggcaagcgc agaagccacg cctggcaccg ttcgtgccac ctcttcaatt
    16381 tgctccgcca tcgcgtcgat atccgccagc acagtgccgg aaacttttat gccgataggg
    16441 cttttaatgc cggttgagag catatcgata cggttacgaa ttggcggcac ccacagattc
    16501 gccagccccg gcagacgcac ggtgttatcc agttcctcaa tgattttgtc cattgtcatg
    16561 cctggccgcc actgctcctg cggcttaagc tggatggtcg tttctaccat ctccagcgga
    16621 gcagaatcgg tggcggtttc cgctttcccg gttttgccaa atacccgcgc cacttcaggt
    16681 acgctcataa ttagcttgtc ggttttttgc agcatactcg ccgcctctgc tgcggaaatc
    16741 cccggcagcg tcgatggcat atacaacaag tcgccttcat tgatctgcgg taaaaattcc
    16801 ccgccaactt tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc caccaacagc
    16861 gtggttttcg gccagtgcag tactttcagc aacagcggat gataaacacg aatcaaaaag
    16921 cgattgagcg ggttactgct ttccggcgga attttgccac ggatccagta acccatcagg
    16981 atagggatca ctacgatcgc cagcagcgcc gcacccgcca tcgcatacgt tttggtgaac
    17041 gccagcgggc caaacagacg cccttcctgc ccttccaggg tgaagatcgg gataaacgac
    17101 agcgtgataa tcagcagact gataaacagc gccggcccca cttcaacaga tgcattggta
    17161 atcacctgcc agcgcgtttt attatccagc gtggcgtcag gatgctggtg ctgccactct
    17221 tccagccgtt tatgtgcatt ctcgatcatg acgatagcgg catcgaccat cgccccgacg
    17281 gcaatcgcaa tgccacccaa cgacataata ttggcattca gcccctggaa gtgcatgaca
    17341 ataaaagcaa tacacaaccc cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca
    17401 tgccagagaa acagcgcaca gactaccgcc accacaataa actcttccag caacttgccg
    17461 ctgaggttgt cgatggcccg gtcaatgagc tggctgcgat cgtatgttgt aactatctcc
    17521 acgccttccg gcagactgct tttcagcgtt tccagtttgt ccttcacggc ggcgatcact
    17581 tcacgggcgt ttttgccgga gcgtaggatc accaccccgc ccacaacttc gccgttcagt
    17641 tcggcaatgc cccggcgcat ctccgggcca acctggatct tcgcaacatc acgcagataa
    17701 acaggcacgc cattttcact ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgt
    17761 agatagccgc tggcgcgcac catatattct gcttccgcca gttcgattga cgaaccgccc
    17821 gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg cgagactgat gccatactgc
    17881 gccaggcgct gaggatcgat aaccacctga tactctttca ccacgccgcc caccgacgcc
    17941 acttccgcaa cgtcagggat ggtttttagc tcatatttga gaaaccagtc ctgcaatgag
    18001 cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca gtgcatattc atagatccag
    18061 ccaacacccg tggcatctgg tcccagctcg gcgctgactc ccgcaggcag cttaccctgt
    18121 acctggttga ggtactccag cacccgcgaa cgcgcccagt acggatcggt accatcttcg
    18181 aaaatgacat acacataaga gtcgccaaac tgcgagaaac cacgcacagt cttcgcgcca
    18241 ggcaccgaca acatggtggt agttagcgga taagtcacct gattttcaac gatttgcggt
    18301 gcctgaccgg gatagctggt tttaataatc acctgcacat cggagagatc cggcaacgca
    18361 tccactggcg tgttaatgat ggtccaggtg ccccagatac tcagaaataa cgcgcccatc
    18421 agcaccaaaa aacggttcgc caccgagcga cgaataatcc attcaatcat tggttattcc
    18481 ctcaatgcgc atgggtagca ctttcagagc gcatccgctc cagtgcgcca gaaatattgg
    18541 cttcggaatc aatcaggaac aggccgctgg aaaccacctt ttcaccttcc gccagaccag
    18601 agcgtaatgc ggtgacacct tgcgatgcct ggaaaacggc aacgcgtttt ggtacaaagc
    18661 gcccgtcggc atcaacggta atcacccgct gttcgctgcc ggtatcaatc agcgcctgtg
    18721 acggaatgag cagcatcggt tcgctggcgg tattgagttg caaccaggcg ttcattcccg
    18781 gttttagcgc ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg
    18841 catccacgcc aggtagtagc gtccatttgc ggatggtgag tgttttatca ggtcgcgccg
    18901 gaacggtgag cgtaaactgc gaggcatctt tcaccagcca ggcgatggac tccgggatcg
    18961 cagcagtgac ccacaccggg tccataccct gaattttcgc taccacgtta tctttggcga
    19021 tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggt gctttgagcg
    19081 taaagcgagt ctggattttt tgcgtggcga tcaggcggcg aatatccgcc tccggcattc
    19141 ccgccagtcg cagccgctca agaatgcctt cggtctgggt cgccgtaccg ccggtttcgc
    19201 gcagcagtaa atactcactc tgcgcttcca cccagtcggg aatggtcagg tcgagaagcg
    19261 gcgcgccctt ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg
    19321 cggcgcgagc ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac
    19381 tctgggcaaa agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg
    19441 tctgagtcgg gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca
    19501 ccagatccat atccataaac ggcgatttac ctggtttatc gaaccgcgta ttgggataca
    19561 tcgggtcgta ccagaataag attttacgtt ctgcggtcga cgttttttct gcaggcggtt
    19621 ccgcctttgc aaaccaggta aaacctgccg cagaaataat accgcccgcg atcatgctgc
    19681 cgataataag cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat
    19741 cctgtaataa agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt
    19801 taattccact cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt
    19861 tcacggcagc aatcggatcg tgatggatgg tgattttttt gctttccaga tcaaaacctt
    19921 ttaccacgcc ggtggcacta atgacctgtg gttgtgcttc gctcatggtt tcatgatgat
    19981 gttcgttagc ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca
    20041 gtgctttttt catttatttt ctcctggagt taaaaattat tgctgccaac cgccaccaag
    20101 tgcggtatat aaagaaattt cgttgacctg acgggcataa ttcaggtcaa gtacggtttg
    20161 tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta ctgcgccgtg
    20221 ctgatataat gcccgcgccc gttgcaaagt aatttgcagc gatgccagat aacgctgctg
    20281 ggcgctaatt tgatcgttca ggctttgacg taatgccagc gcatctgcca cttctttaaa
    20341 ggcgttctgg attttctgtt cataattcac taccgactgc tgctggcgaa tttcggcgat
    20401 atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt taggaataaa
    20461 attccacatc ccgctgctgg cgttaaataa tgacgacaga tcgctgctgg cggttgatat
    20521 tccgctggtc aggctgatag acgggaaaaa tgccgcgcgt gcagcaccaa tattggcatt
    20581 agccgccatt aacgcatgtt cagcttccat aatatccggg cgctgcaata agatttgcga
    20641 cgacaagccc gccggtaatt taacgctttg caggctgtcg ctgtttactg tctgcgcttg
    20701 cggcagcttg ccgtagcttc ccagtaacag ttgcaatgca ttattcgcct gcgccagttc
    20761 cccctgacgt ttagcgatgt cgctgcgggt actttcaatc accccgcgag cctgttccag
    20821 cgccagaaca ttactgctac ctgtcaacag ttgtttttcg acaaacgcat atgactgctg
    20881 ataattacgc agcgtttctt cggctatttg caattgcgca tacgccagct gctgattgaa
    20941 atagctttgc gcgacattag aaaccagcag aatatgcacc gcgcgctgag cttcctcagt
    21001 ggctaaataa ttttgtcgct cggcttcgct catgttcttt aagcgcccga aaaaatcgag
    21061 gtcaaagctg gcgttaaggc cagtagaaaa ctcccgcgtc gtggctgaat cgcctttaag
    21121 attgccgctc cagctgccgc tgccctcacc attgagctgt gggtagcggt cggcatcagt
    21181 cagacgatat tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccggtt
    21241 attctccagc gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca
    21301 gcccgcgttc tgatagttat ctgctgcgtt aaccaggccg ttctggctga gtgagaactg
    21361 ctgcggtacg ggcattgccg ggcgctgata atccggtgcc agtgaacaac cggttagcgc
    21421 aagggccaca caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat
    21481 tttataccgc caactttact cgccaggctc tgattttccg gtgacaggaa aatgacaaaa
    21541 ttgtcatttt gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca
    21601 acatgcggag gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac cggagaatac
    21661 ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggctgaat
    21721 ggctaccatc tggcgatgac cggagattat gacctgataa tcctcgatat tatgctgccg
    21781 gacgtgaacg gctgggatat cgtgcgcatg ttgcgctccg ccaataaagg gatgccgatt
    21841 ctgttgctta ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggcc
    21901 gatgactatc tggtgaagcc gttcgcgttt gctgaactgc tggcgcgggt gcgcactctc
    21961 ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc aggttgccga tttgatggtc
    22021 gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac cagtaaagag
    22081 tttactctgc tggagttttt tctccgccat cagggcgaag tgctgccccg ctcgcttatc
    22141 gcctcgcagg tatgggacat gaattttgac agcgacacta acgccattga tgtggcggtg
    22201 aagcggctac gcggcaaaat cgacaacgac tttgagccga agctgattca aaccgtgcgc
    22261 ggcgtgggtt acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc
    22321 gctggcaacc cgcctgacct tttttatcag cctggccacc atcgcggcgt ttttcgcctt
    22381 tgcatggatc atgatccact cagtaaaagt gcattttgcc gagcaggata ttaatgattt
    22441 aaaagagatt agtgccaccc ttgaacgcgt actaaatcac cctgacgaaa cgcaagcccg
    22501 acgcttaatg acgctggaag atatcgtcag tggttattcc aatgtgttga tttccctggc
    22561 agatagccac ggtaaaacgg tgtatcactc ccccggtgcg cctgatatcc gcgagtttgc
    22621 gcgcgatgcc atacccgata aagacgcgcg gggcggcgag gtgtttctcc tctccggccc
    22681 gacgatgatg atgccaggcc acggtcacgg gcatatggaa cacagcaact ggcggatgat
    22741 cagtttgcca gttggcccac tggttgacgg caaaccgatt tatacgctct acatcgcgct
    22801 ttcgatcgat tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc
    22861 atcggtcatc agcatcctga tcgtctttat cgtgctattg gcggttcata aaggtcacgc
    22921 gccgatccgc agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc tcgacgtccg
    22981 ccttgacccg cagaccgtgc cgattgagct ggaacagctg gtactgtcgt tcaaccatat
    23041 gatcgagcgt attgaggatg tctttacccg ccagtccaat ttctcagcgg atatcgccca
    23101 cgaaattcgc acaccaatta ctaatctcat aacgcaaacg gaaatcgccc tcagccagtc
    23161 gcgcagccag aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat
    23221 ggcgaaaatg gtcagcgata tgctgtttct cgcccaggcc gataacaacc agttaatccc
    23281 cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc
    23341 tttagcggaa gatcgcggcg tggagctgca gtttgttggc gacgagtgcc aggttgcggg
    23401 cgatccgctg atgctgcgtc gggcgttaag caacctgctt tcgaacgccc tgcgttatac
    23461 gccacccgga gaggctattg tagtgcgctg ccagacggtc gatcatctgg tgcaagttat
    23521 cgtcgaaaac cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt
    23581 ctaccgcgtt gacccctccc gccaacgaaa aggcgaaggt agcggcattg ggctggcgat
    23641 agtgaaatca attgttgtcg cgcataaagg cacggttgcg gttacgtcaa atgcgcgggg
    23701 gacaaggttt gtgattgtgt tgccggagag ggggtgatta ttaagttcaa attcagggcg
    23761 agggtttacg gcgtttttcg ctaaatttcc atcattataa acaagccacc tatcatataa
    23821 agcaatgaat aattaaatcg cgattaat
//
LOCUS       DAZKIF010000076        23385 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZKIF010000076 DAZKIF010000000
VERSION     DAZKIF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.076"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..221
                     /gene="ypdI"
                     /locus_tag="ACHOTW_002885"
     CDS             <1..221
                     /gene="ypdI"
                     /locus_tag="ACHOTW_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFP9844951.1"
                     /translation="NVFAFSNSGGGSETSYKEPEKTSAMTTTHSTKFQPSQAILFKMR
                     EDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(298..540)
                     /gene="yfdY"
                     /locus_tag="ACHOTW_002886"
     CDS             complement(298..540)
                     /gene="yfdY"
                     /locus_tag="ACHOTW_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP9844952.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            893..1813
                     /gene="lpxP"
                     /locus_tag="ACHOTW_002887"
     CDS             893..1813
                     /gene="lpxP"
                     /locus_tag="ACHOTW_002887"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP9844953.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2169..2240
                     /gene="ypdK"
                     /locus_tag="ACHOTW_002888"
     CDS             2169..2240
                     /gene="ypdK"
                     /locus_tag="ACHOTW_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP9844954.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2305..3543)
                     /gene="alaC"
                     /locus_tag="ACHOTW_002889"
     CDS             complement(2305..3543)
                     /gene="alaC"
                     /locus_tag="ACHOTW_002889"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP9844955.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            3920..5617
                     /gene="ypdA"
                     /locus_tag="ACHOTW_002890"
     CDS             3920..5617
                     /gene="ypdA"
                     /locus_tag="ACHOTW_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP9844956.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5632..6366
                     /gene="ypdB"
                     /locus_tag="ACHOTW_002891"
     CDS             5632..6366
                     /gene="ypdB"
                     /locus_tag="ACHOTW_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP9844957.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6379..7236
                     /gene="ypdC"
                     /locus_tag="ACHOTW_002892"
     CDS             6379..7236
                     /gene="ypdC"
                     /locus_tag="ACHOTW_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9844958.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7239..9734)
                     /gene="ptsP"
                     /locus_tag="ACHOTW_002893"
     CDS             complement(7239..9734)
                     /gene="ptsP"
                     /locus_tag="ACHOTW_002893"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9844959.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9759..10796)
                     /gene="ypdE"
                     /locus_tag="ACHOTW_002894"
     CDS             complement(9759..10796)
                     /gene="ypdE"
                     /locus_tag="ACHOTW_002894"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9844960.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10796..11881)
                     /gene="ypdF"
                     /locus_tag="ACHOTW_002895"
     CDS             complement(10796..11881)
                     /gene="ypdF"
                     /locus_tag="ACHOTW_002895"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9844961.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11896..13143)
                     /gene="fryC"
                     /locus_tag="ACHOTW_002896"
     CDS             complement(11896..13143)
                     /gene="fryC"
                     /locus_tag="ACHOTW_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9844962.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13165..13491)
                     /gene="fryB"
                     /locus_tag="ACHOTW_002897"
     CDS             complement(13165..13491)
                     /gene="fryB"
                     /locus_tag="ACHOTW_002897"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9844963.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13710..14675)
                     /gene="glk"
                     /locus_tag="ACHOTW_002898"
     CDS             complement(13710..14675)
                     /gene="glk"
                     /locus_tag="ACHOTW_002898"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP9844964.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14879..16135
                     /gene="yfeO"
                     /locus_tag="ACHOTW_002899"
     CDS             14879..16135
                     /gene="yfeO"
                     /locus_tag="ACHOTW_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP9844965.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16250..16576
                     /gene="ypeC"
                     /locus_tag="ACHOTW_002900"
     CDS             16250..16576
                     /gene="ypeC"
                     /locus_tag="ACHOTW_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP9844966.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16716..17954)
                     /gene="mntH"
                     /locus_tag="ACHOTW_002901"
     CDS             complement(16716..17954)
                     /gene="mntH"
                     /locus_tag="ACHOTW_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9844967.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18290..19492
                     /gene="nupC"
                     /locus_tag="ACHOTW_002902"
     CDS             18290..19492
                     /gene="nupC"
                     /locus_tag="ACHOTW_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP9844968.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19542..21731)
                     /gene="pdeA"
                     /locus_tag="ACHOTW_002903"
     CDS             complement(19542..21731)
                     /gene="pdeA"
                     /locus_tag="ACHOTW_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9844969.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(21939..22014)
                     /locus_tag="ACHOTW_002904"
     tRNA            complement(21939..22014)
                     /locus_tag="ACHOTW_002904"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21979..21981),aa:Ala,seq:ggc)
     gene            complement(22054..22129)
                     /locus_tag="ACHOTW_002905"
     tRNA            complement(22054..22129)
                     /locus_tag="ACHOTW_002905"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22094..22096),aa:Ala,seq:ggc)
     gene            22350..22709
                     /gene="yfeC"
                     /locus_tag="ACHOTW_002906"
     CDS             22350..22709
                     /gene="yfeC"
                     /locus_tag="ACHOTW_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9844970.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22711..23103
                     /gene="yfeD"
                     /locus_tag="ACHOTW_002907"
     CDS             22711..23103
                     /gene="yfeD"
                     /locus_tag="ACHOTW_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9844971.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23143..>23385)
                     /locus_tag="ACHOTW_002908"
     CDS             complement(23143..>23385)
                     /locus_tag="ACHOTW_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9844972.1"
                     /translation="LAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNG
                     ARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         5803 a         6206 c         5785 g         5591 t
ORIGIN      
        1 gcaatgtttt tgcattttct aattctggtg gtggtagtga gacgagctat aaagagccag
       61 aaaaaacgtc agcgatgacg acaactcact ctacaaaatt tcagccatca caggcgattt
      121 tgtttaagat gagagaagat gcaccaccat taaacctcac agaagaaacc acgccccctt
      181 ttccgacaaa ggcgaattat cttattcatc ctgtgcgata gcgtctgtca cctggcaggt
      241 gaacaatcat gtctgatgct gatgtgaaca cctgcccaaa tgaaccatcc tgacccacta
      301 tctcctgcca taccccacca taaagcgaat catcaagcca caagccgggg caagaaacat
      361 aaatatcccc gtcaccagtt cattaccgcc agttaaccac atggtgaagt aataaatgag
      421 tgcagcgagg atcaccatgc cgaagaaaga agagaccgcc gataccacgt tcagtacctg
      481 accgatgtat ccgcttacgc agacaataca cagggcgaga aacatccata aataaaacat
      541 agctcaacct cctgccgcgc taaaaaacct gtaacttcag tcactttaat agtaagacaa
      601 tccttaacct ctggcaattt ttgctaagcg tcaatgaaaa cagcatgtta aatgcaatac
      661 tattgcgtgc ggaaaatttt ttactttgca cgattaataa tcagtagctg aaagcagtca
      721 gccagtgata aagtacagat accgcattca tgctcctcgt ggttatgtcc tgactagtct
      781 ttacactctt tacaggaacc attgtcgtac atgatggccc aaccaattga gtgtaccgct
      841 gtcgacatgg aatcttctgc gatacaacaa ttcgtatcta cagaaggtaa ctatgtttcc
      901 acaatgcaaa ttttcccgcg agtttctaca tcctcgctac tggctcacat ggtttgggct
      961 tggtgtactc tggctttggg tacagcttcc ttatcctgtt ctctgctttc tcggcacgcg
     1021 tattggcgca atggcgcgac cattcctgaa acggcgtgaa tctatcgccc gtaaaaacct
     1081 ggaactttgc ttcccgcagc attctgcgga agaacgcgag aagatgattg ccgaaaactt
     1141 tcgttcactc ggcatggctc tggtagaaac cggcatggcg tggttctggc ccgacagtcg
     1201 cgtacgtaaa tggtttgatg ttgaagggct ggataacctt aaacgcgcac aaatgcaaaa
     1261 tcgcggcgta atggttgtcg gcgtccattt tatgtcgctg gaactgggcg gccgcgtgat
     1321 gggactttgt cagccaatga tggctaccta tcgtccacat aataatcagc tgatggaatg
     1381 ggtgcagacc cgtgggcgca tgcgctctaa caaagcgatg atcggcagaa ataatctgcg
     1441 cggcattgtc ggtgcactga agaaaggtga agcggtatgg tttgctcccg atcaggatta
     1501 tggtcgtaaa ggcagctcct tcgcgccgtt ctttgcggtg gaaaatgtcg ccacaaccaa
     1561 tggcacctat gttctctccc gtctctctgg cgcagccatg ttgaccgtaa cgatggtaag
     1621 aaaagcggat tacagcggat atcgtttgtt catcacccca gagatggaag gctacccgac
     1681 agatgaaaat caagccgctg cctatatgaa caagattatc gagaaagaga tcatgcgcgc
     1741 accggagcag tacctctgga tccaccgtcg ctttaaaacg cgcccggtgg gagaatcgtc
     1801 gttgtacatt taacaaactg ctcttactct ttccctcctc aaatcggcca taataacctc
     1861 atggttttta tggctttttt cttaagattt tttccttcgc tgaatatcta attattaccg
     1921 ccagaaatta aactgtcgcc gatatgagac acctgtaagt tactaaaagt aaataagaaa
     1981 atgcctcttg tctcgtttca gttttaggcg tacattagcg ccgtctcgag cagtttagct
     2041 cagaattctg gggtgatgcc cacggtaaaa tacaggactt ttaaggtcgt tttgcccggt
     2101 gtcaactcac tgtatcaggt gtgatgaagt cattcaggcg taaccgtaat tacgcggaga
     2161 gatgtaaagt gaaatatttc tttatgggca tttcttttat ggtcatcgtt tgggccggta
     2221 cttttgccct gatgatctaa aagcaaactg tgcaatgaaa aacaggagcc atcaggctcc
     2281 tgtttgcatt tctgacgacg gtgtttattc cgcgttttcg tgaatatgtt tgctgctggc
     2341 gggtagtaaa ccgtcggcac ggaacatcgc tttaatccca cgaatcgcct gacgaatacg
     2401 gtcgcggttt tcaatcagtg caaagcgaac atgcgtgtcg ccgtagtcac caaagccaat
     2461 ccctggagag acacagacct tcgcttcgtt aaggagcttc ttggcaaatt ccagcgatcc
     2521 catggccgca tatggttccg ggattttcgc ccagacatac atcgaagcct tcggcatttc
     2581 gaccatccag cccgcttcat gcagcccttt aaccaataca tcgcggcggc gtttgtactg
     2641 ttcggcaatg tcgcgcacgc actgttgatc gccctccagc gccgcaatcg ccgccacctg
     2701 caacggcgta aaagtgccgt aatcgtgata gcttttgatt cgcgccaggg cactgaccag
     2761 cgttttgttg ccgaccataa agccgatacg ccagcccgcc atgttgtagc ttttcgacag
     2821 cgtaaagaac tcgactgcca catcgcgtgc acccggtacc tgcatgattg acggcgcttt
     2881 ccagccatcg tagacgatat cggcataggc caggtcatgg accaccagca catcgtagcg
     2941 tttcgccagc gccactactt tttcgaagaa ctccagttcc acgcattgcg cggttgggtt
     3001 tgacgggaag ccgaggatca tcatcttcgg tttcggataa ctttcaagaa tggcgcgctc
     3061 cagttcgttg aagaaatcga caccttccac cagcggcact gagcgtacct gcgccccggc
     3121 aatcaccgcg ccataaatat ggatcgggta gcttggattc ggcaccagca ccgtgtcacc
     3181 atgatccagc gtcgccagca tcaggtgtgc cagcccttct ttcgaaccaa tggtgacgat
     3241 ggcttctgat tccggatcga tttcaacgtc gtagcgatcc tgataccagc gggaaatggc
     3301 gcgacgtaac cgcggaatgc cgcgtgaagt ggagtaacca tgcgtgtccg gacgctgggc
     3361 cacagtacat aatttttcga cgatatgcgg cggagtcgca ccgtccgggt tacccatgct
     3421 gaaatcgata atatcttcgc cgcgccgacg cgcagccatt ttcagttcag cggtgatgtt
     3481 aaaaacgtag ggcgggagac gatcaatgcg cgtaaagcga cgttcagggc gagtgtcagc
     3541 cataatttcc tcagattaac gttagcgccc ggaccgtccg agcgacgctg ccacgatggt
     3601 ggctgttttg aaaatagcct gattaatttc tgtctgtcca gcgttttaga acatttttat
     3661 taatgacgtt aaatgaggaa ctctgcttta aaaacaggaa atttatctgc gctaacaaga
     3721 aaattaccac tcattttatt aacataataa tttcaataaa tttactaacc agaagtgcat
     3781 tatcaagaga tgcattgcta atacaccatt tatctatcaa tcccctttta aaattgtggt
     3841 ttttcctcaa aaacggcctg ctggtcatca gccttcaggt tacctatcat agaggtttaa
     3901 tccttattca gagtcacccg tgcacgaaat attcaacatg ctgctggcgg tcttcgatcg
     3961 ggcagcgtta atgcttatct gcctgttctt tctcatccgt atccgcctgt ttcgcgaact
     4021 gttgcacaag tcggcacact caccgaaaga gttgctcgcc gttaccgcca ttttctcgct
     4081 gttcgccctc ttcagcacct ggtccggcgt tcccgtagaa ggctcgctgg tgaacgtacg
     4141 tattatcgcg gtgatgtccg gcgggattct gtttggcccg tgggtgggca tcattaccgg
     4201 cgtgattgcg ggtattcatc gttatttaat tgatatcggt ggcgtgacgg cgatcccctg
     4261 ctttatcacc agcattctgg ctggttgtat atcgggctgg atcaacctga aaatccccaa
     4321 agcacagcgc tggcgcgtcg gtattctcgg cggcatgttg tgtgaaacgt tgaccatgat
     4381 tctggtgatt gtctgggcac caactaccgc gttggggatc gatatcgtct ctaaaatcgg
     4441 gattccaatg atcctcggca gcgtctgtat cggctttatt gtgcttctag tgcaaagcgt
     4501 tgagggcgaa aaagaggcca gcgccgcgcg gcaggccaag ttggcgctgg atatcgccaa
     4561 caaaacgcta cctctgtttc gccatgtcaa tagcgagtca ttacgcaaag tttgtgaaat
     4621 tattcgcgat gacattcacg ccgatgcggt ggcgattacc aataccgatc atgtgctggc
     4681 ctatgttggt gtgggtgaac ataactatca gaatggcgat gacttcatta gcccgactac
     4741 ccgtcaggcg atgaattacg gaaaaatcat cattaaaaac aatgatgaag cccaccgcac
     4801 accagagatt cattccatgc tggtgatccc attgtgggag aaaggcgttg tgaccggaac
     4861 gctgaaaatt tactactgcc acgcgcatca gataacctcg tcattacagg aaatggcggt
     4921 tggtctgtcg caaatcatct ccacgcaact ggaggtttca cgcgccgagc agctacgtga
     4981 aatggcaaat aaggcagagc ttcgcgccct gcaaagcaaa attaatcccc atttcctgtt
     5041 taacgctttg aacgctattt catcgtcgat ccgtctgaat ccggataccg ctcgccagct
     5101 gatctttaat ctgtcgcgtt atctgcgcta taacattgaa ttaaaagacg atgagcaaat
     5161 cgatatcaaa aaagagctgt atcaaattaa agactatatt gccattgagc aggcccgctt
     5221 tggcgacaag ctgacggtta tctatgatat tgatgaagag gtgaattgct gcattcccag
     5281 cctgctgatc cagccgttgg tggagaatgc cattgtccac ggtatccagc cttgcaaagg
     5341 taaaggcgtt gtcaccatca gcgttgcaga gtgcggcaat cgggtacgca ttgcggtgcg
     5401 ggataccggg cacggcatcg atccaaaggt gattgagcgg gtcgaagcga atgaaatgcc
     5461 gggcaataaa attggcctgc tgaatgtcca tcatcgcgtg aagttattgt atggcgaggg
     5521 gctgcatatc cgccgcctgg aaccaggaac ggaaattgcg ttttacattc ctaaccaacg
     5581 caccccagtc gcctcacagg ctacgttatt gctttgagcc ggagtgatat tgtgaaagtc
     5641 atcattgttg aagacgaatt cctggcacaa caggaactga gctggctaat taaagagcac
     5701 agccagatgg agattgtcgg cacctttgac gacggtctgg acgtgttgaa gtttttgcag
     5761 cataaccgcg tcgacgccat ttttctggat atcaatattc cgtcgctgga tggtgtgttg
     5821 ctggcacaaa acatcagcca gttcgcccat aaaccgttta ttgtgttcat caccgcgtgg
     5881 aaagaacatg cggtagaagc gtttgaactg gaggcgtttg actacattct aaaaccgtat
     5941 caggagtcac gcattaccgg gatgctgcaa aaactggaag cagcctggca acaacagcag
     6001 accagcagca cgcctgccgc gacggtaacg cgtgagaatg acaccattaa tctggtgaaa
     6061 gatgagcgaa taatcgtcac gccaattaac gatatctatt acgccgaagc gcacgagaaa
     6121 atgacctttg tttatacgcg gcgtgaatcc tacgtaatgc cgatgaacat taccgaattt
     6181 tgcagcaaac tgccgccgtc gcattttttc cgctgccatc gctcattttg tgtcaatctg
     6241 aacaaaatcc gcgaaatcga accgtggttt aataacacct atattctgcg gctgaaagat
     6301 ctggattttg aagtgccggt cagccgcagc aaagttaaag aattccgcca gttaatgcat
     6361 ctttaatcgg gaactttcat gaaagcacca ggcttgcccg ccgatcaaca atttttcgcc
     6421 gatctgttca gcggcctggt gcttaacccg caactactgg ggcgcgtctg gtttgccagc
     6481 cagcctgcct cgttgccggt gggcagttta tgtattgatt ttccccgtct ggatatcgtg
     6541 ctgcgcggcg aatacggcaa tctgctggaa gcaaagcagc aacgtttggt ggaaggagaa
     6601 atgctgttta ttccggcgcg cgcggctaat ttaccggtca acaacaaacc ggtgatgctg
     6661 ttaagcctgg tgttcgctcc gacctggctt gggttatcgt tttacgatag ccgcaccacg
     6721 tcattgttgc atcctgctcg ccagatccag cttcccagcc tgcaacgcgg tgaaggtgaa
     6781 gcgatgctta ccgccctcac ccatcttagc cgttcgccgc tggagcaaaa tatcattcag
     6841 ccgctggtgt taagtttgct gcatctttgc cgtaacgtgg tgaatatgcc gccgggcaat
     6901 tcgcagccgc gcggcgattt tctctatcac agcatttgta actgggttca ggataattat
     6961 gcccagccgc tcacccgcga gagcgtggcg cagtttttta atatcacgcc caatcatctg
     7021 tcaaaactgt ttgctcagca tggaacgatg cgttttatcg agtatgtgcg ttgggtgcga
     7081 atggcgaagg cgaggatgat tttgcagaaa tatcatttgt caattcatga agtggcacag
     7141 cgttgcggtt ttccggatag cgactatttt tgtcgcgttt tccggcgtca gtttggtctg
     7201 acgccgggag agtacagcgc ccgttttcag ggctaacgtc agaaggttaa ttctgtttcc
     7261 agcagcgtca ggatactttg tgcatcttgc gcggcaaaca acgactggcg gaagttttta
     7321 ttcaccagtt tacgcgccag ctgcgagaag actttcacat gattcatccc ttcgttagca
     7381 cccagcgtca gcatgatcac cagttcgact tcgcccattt ctgactgcca gtcaaccggt
     7441 ttcgccagcc gggcaatgct gatactggaa tgacggatcc actgagattt ggtgtgcggg
     7501 atcgccacgc caaaaccaac gccagtggtg acaatctctt cccgctgcca gacatcctct
     7561 tccagctcga acggatgttc agtgcgcccg ttaacgccga ggttaccgca caggaactgg
     7621 atcgcctgct ctttattgct aaaggcctga tcaacgaaga tattctccag cgccagcagc
     7681 gggcgaacgt cttcttccgg cgtaaaggcg gtgagtaacg cttcaatttc ctgcgcgctg
     7741 cgacattcac acgcctgacg tgccagttcc cgacacgcct cgctatccag ttgacgaagc
     7801 tggcttttca ccgccggaat gcgcgggcta ctcatactca gctcatccag gcccagccca
     7861 agcagtagcg gcagataacg gctttcaccg cccagttcac cgcaaatgcc tacccatttg
     7921 ccccgctgat gcgcagtggt aactatttgc tgcaacatgc gcaggaacga tggcgtaatc
     7981 gggttatata gcggcgatac gcgcgggtta ttacgatcga ccgcatacag atactgggtc
     8041 atatcgttgg agccgatact gaagaaatcg acctcatcgc agaagtgatc gatgatgtag
     8101 cacaccgacg gcacttccac catgataccc agcgtaatcg tctctgcatg acgcaggcca
     8161 tcgcgcttaa gctcaacgat cgctttttgg atctcgccct tcacccataa gatctgatcg
     8221 aggccgtgga ccatcgggat catcaactgg gcgttgccga aactggcggc gcgcaaaatg
     8281 gcccgcagtt gagtgcggaa caggccagca aattccgggt aaatacgtac cgcgcgatag
     8341 ccgaggaacg ggttctcttc ctggggaata ttcagataag gaatgctttt atcgcctccg
     8401 atgtccattg tgcggaagat aatcggcttg tcgcccgctg ccagtagcac ctgctggtag
     8461 gcttcaaatt gctcctgctc gtccggctcg ctgtcgcgat ccatatagag catttcggta
     8521 cggaacagcc cgacgccttc cgcgccgttg gcaaacgcgc caggcgcttc cagagcggtg
     8581 ccaatattcg ccgcgatatc aatacgcttg ttatcacggg aataagccag ctgcgcggca
     8641 gcctgcgcct gttgtttttg gcgtttatcc gccagcgtct gcgcgacctg ataataaccg
     8701 ctcaccgcgt cattcgggtt aatcgccagc acgccgcact gggcgtcaag cactgcaggt
     8761 tgcccggcat aacgcgcgat cgcatccagc ggcaggccgc tcagtaccgg gatcgccgag
     8821 gcgcgggcca gaatcagggt atgcgaggtg cgcccggttt tctccagaat catgcccgca
     8881 agatttttta aatccaggct taaaaactgg cttggggtta aatcctcggc caccagaata
     8941 gtcggttttt ccagcaccag attgttgcgc ggcttcagtt ccggccaggt aatatgcagc
     9001 aactgttcgc taatatcgcg aatgtcgcta acacgttcgc gcagataatc gctggcagaa
     9061 gcagaaagtt tggcgcaaac ctgctccata tttctgatga tcgccgcccc cagcccctga
     9121 tgctgttctg tcatcaggcg acggatattg cctgcaaatt catcatcctg aatcagcgac
     9181 aaatgggcgc tgaggatagt tttgctttcg ccgtcacgct cacgcaattg ctggttcagt
     9241 tgctcggcaa gcgttgccag gctgtgctcc agccgggtgg aatcttgcgc actggcgggg
     9301 attgcccgat aactgtcgag gctgtcgctt tgtaacaggg tcagcgtacc cacgccgacg
     9361 ccgcttgcca gcacattgcc gtacagtaaa tccgggttaa ggcggcttaa tgaacgcggc
     9421 agcggatgcg ccgtcagttc tgcctgcgta ggctgaacgc tgtcgctgtc gataaagcgc
     9481 acctggatgt actcttccag cacgcgccgc gcctgctctt catcgctgcc gctaatgttc
     9541 aggctgcaac tgtcattaaa tagggtgccg gtgccaatca gcgccagcga gcttttggca
     9601 tcggcctttg cgttctggcg atggttaata aaagtgattt cgctttgcca ctggctgcac
     9661 tgttctttaa gttcccacgc cggacgagca tgtagaccgt taggcaaagg acagagaaat
     9721 tgaatcgtta acataaccgc tccgggttaa tcaggagatc atctgaaatc cgtcagttga
     9781 acaaccgtct cacgcgtaag acgttgaata agggcagata aaagttgctc catctgcaaa
     9841 atgtcacggc aatcggcaat cgacgcggcg caatgtccgt ggcgggtggc aggccccatc
     9901 accactgtgg gtacgccagt accggttaag tgcaccgctc caccgtccgt gccgccgtta
     9961 ctgaacatat ccgcctgtaa cggcacgcca atttctgccg ccacggtttc gatccaggcg
    10021 gtgagttttg gcggcgcaat cagtgacttg tcgcttaaca ccagcatcgg gccgttacca
    10081 atctggcgat ggttagccgc gccataatta aagtttttcg cccagcaggc ggtatcaagg
    10141 acaatggcga cgtccggcga caccgcgcgg gtggcagttt gcccgccgcg taaccccacc
    10201 tcttcgctgg aactggccac cagccacact tccgcaggca gttcagcgct gtgtagttcg
    10261 cgcagtaacg tcaccagcag gtagcaaccg aggcggtcat caaaggcttt ccccatcacc
    10321 cgctggtggg ggagaacctg aaaagtggta tcgaacgtga cgcgatcgcc tggacgaatt
    10381 cccgcctgca tcacttcgtc atacgagcgc gcgccaatat ccacgcgcat ggcgctgaca
    10441 tcattcccct gccggtcgcc gtcaagcagg cctggaattt tgcactcttc acgggtggtg
    10501 atgcgcaccg gctgcagctg gcgggcagcc atgcgtacat tgccaaccgg cagcacatca
    10561 atcgcccctt cgcgggagat gctgcgcacc ataaatccca cttcgtccat atgcgcacag
    10621 atcatcacct tcggacctgt cgattcattg aggcggatca gcaccgatcc cagcccatca
    10681 aatcgcactt ctttttgcag gcgatccgct tcttccagca ggatctgccg cacttcctgt
    10741 tccgaggagg cgatcgcatc tgcctcgctc aacgctttca atagcgataa atccattatg
    10801 cctctcccgt gagcaacact gttttcggca tggcgtagag cacttctgcg ccttgcgggg
    10861 tgaccagcac aacatcctcg atgcgcacgc ccccttgccc tggcaaataa atccccggct
    10921 ccacggtcag taacatgcct ggctgtagcg tcgtggtgtc ccgcggtgaa aaacgcggat
    10981 cttcatgaac ttcaatgccg atagcatgag cggtgttatg accgaaatag tggctaaaac
    11041 ctgcctcggt aatcacccga cgcgcggctt cgtcaacctg ctggcagcgc acgccggggc
    11101 gaattgcaga gattgctgcg agctgtgcct gcaaaacaat ctgatagacg ttaaacagcg
    11161 ggtgagattc ggcgctcacc ccttcgccat tcaccagcaa ggtgcgcgtc atatcagagc
    11221 agtagccctg atagagcgca ccgaaatcga gagtgacaaa ctcgcccgct gcaacaatct
    11281 tgtcgctggc tttgccgtgc ggcagcgccc cacgccagcc actggcgaca atggtgtcaa
    11341 aagaggtttt ttctgcgccc tgctggcgca taaaccactc cagttcagcg gctatctcgc
    11401 gctcgctcat ccccgcctga ataaagcggc gaatatgctc tgcaccgcga tcggcaatcc
    11461 cacaggcaag gcggattttc tccacctcct ctggcgtttt gatttgccgc agcacatccg
    11521 gcgtggcgct aaccagtttc gcattgagtt cagactgcca gcgatgcgcg gtttcccaac
    11581 tcacctgctg gccttcaaaa ccgagcgttt gcaactgctc atcggcaatg atttgcctgg
    11641 cgatggttgt aagcgtgtgc gtcgcgtcaa gcaaatgcag ctggtaacct tgcgtgcggg
    11701 cttctacatc cgcgtaatag cgcgagtcca ccagaatgtg cgcactttca cggctaatca
    11761 gcacatagcc tgatccggtg gagatcccca gatgcggctg tttgttctgc cgtgaggaga
    11821 gaagcaccgc atccagttgt tgcgccttaa gccagtcgcg cagcgaagcg agtaatgtca
    11881 tctggatttt ccttcttaca ggctatcgat taacaatttg cctttacgga acatcatcag
    11941 acgcaggaac accaccatca gcgcagttat gactgccccc agcgcgatcc ccgccatata
    12001 gacgcccagg ttagtgacca gcggccatgc ccagatagcg gattccggga accattgcac
    12061 tgcgcccagc cagacagcgg cggttgagcc gacaatcgcg ccgaccatat acgacggaat
    12121 ggcggtgatg gggctttcca gcgcaaacgg aatcgcaccc tcactgatcc ccataaaggc
    12181 aaggaacatc gcggtcttac cctgcggata aagttgtgcg ttgaacaggc gtttgccggt
    12241 taaacgacgg tcaataatgg tcgccagacc cagaccaatc ggcggaataa cgatagcgat
    12301 agaacgagcg gtgaccggta atacgtggtc agtggtaaag ctgaaggcaa caaaacctgc
    12361 ggctttgttg atcgggccac caaggtcgat tgctgtcgcc gcggcgatcc ccattgcgta
    12421 catcagcgcg cccttctcac ctgcggcagt cagcacagta cggataccgc cgttgatcca
    12481 gcccccaaag ggggtgatga cgtagtacat cgccagcatg acaaaaatag cggaaagaat
    12541 cggtagcaaa aatgtggttt tgaacgctaa caggaaatcc ggtaactgga ttttctggtt
    12601 catccatttc acaaggtaac cggcaacgat agaaataatc agcgcaccaa tgaaggtgga
    12661 cggcaccggc gaagaggtcg cccattgcat agtgctggga tcgaagttca gcagttgcgt
    12721 cggctgggta gacatcaagc cgccgataaa accagccgga aacgccagtt tgccaccgat
    12781 agagttcgcc acaaaagcag caaacatcgg aatggcaaaa ccaaacaaca cgccgccgaa
    12841 ggactgcgac aaccaggcaa atttcagtaa ggagaggtcg aaaccggaga atttcccgct
    12901 attaagagca tccatgatgc cgatctcagc aggaatttta agccagctat aagcaatcaa
    12961 ctggctgaaa gcgaggatca caccgcccat aattaaggtc ggcaccatgc gtgaaatccc
    13021 tgacatcaca tgctgcggga gttcacccca gaagctgctt ttagaggcct gcggattctt
    13081 aactgccgct gccgcaccgg atgcgccagg cacaacggtt gcactgcgtt ttttaatggc
    13141 cataacaatt atccctgtcg aatattactg ttgttcagag gcaatcatct cttcgatttc
    13201 tttgatgatg cccgcagcgt ttttaattgc gtcctgcaaa gtgatttcat aaacgtcgcg
    13261 tgattcgaaa cgttcgttat cttccggggt aactgccacg gagtggatga tgatggtcgc
    13321 ttcggcgata tcctgcgccg tcaggcgatt ctggataccg tccgcgccct gggtttcaat
    13381 tttcacttca taaccggctt ctaccgccgc ttcttccagc gcctgagcgg ccataaaagt
    13441 gtgagccagg cccattgggc aggcacataa ggcaatcagt ttcttactca tcgttaattc
    13501 ctcgttcagt gatagttgac gagtgaaatt atgaacgtgc aaaacaaatc gccgttacca
    13561 gacaaataat gcatttactg gagaattaat cccgctgtac ggaagtgtga cgcaggtcgg
    13621 ccttgtgagg ggacacaaag aaaagttgaa ttaactgtat gatttaaaag attatcggga
    13681 gagttacctc ccgatataac aggaaggatt tacagaatgt gacctaaggt ctggcgtaaa
    13741 tgtgcaccgg aaccgagaag acccggattg tcatggacga tgagatacac cggaatatca
    13801 tggacatatt ctttaaagcg ccctttatct tcaaatgcgg cacggaaacc agaggctttg
    13861 aagaactcaa ggaagcgcgg cacgataccg cccgcaataa acacgccgcc aaatgtcccg
    13921 agattgagcg ccagattgcc gccaaaacgg cccataatga cgcaaaacag cgacaacgca
    13981 cggcggcaat cggtgcagct gtcagccagc gcgcgttcgg taatatcttt tggcttgaga
    14041 ttttctggca ggcggttgtc ggctttaaca attgcgcgat acaaattcac cagtcctggg
    14101 ccagaaagca cgcgttccgc cgaaacatga ccaatttccg cacgcaatat ttcgaggata
    14161 atggcctctt cttcactatt cggcgcaaaa tcaacgtgac cgccttcgcc cggcaagctt
    14221 acccaacgct tatcgacatg gaccagatgc gcaaccccaa gccccgttcc ggcaccgtaa
    14281 accgcaatag gcttaccttc taccggttct gcgccaccaa actgaatcag atgctctttt
    14341 ttcagcatcg ggatcgccat cgatacagcg gtaaaatcgt taataatttc cagatggcta
    14401 aaaccgagat tctttttcat ttcggcaatt gagaacgccc aggtatggtt ggtcattgcc
    14461 acccagtctc cggtaatcgg gcaagcgatg gcaatacagc cgtctttcac ctcgacctta
    14521 tgttcttcaa gataaacgcg aatgaccgct tcgaggctgg ggtaatcaag ccctgaatag
    14581 gtcttagcct gcgagatttc accactggca atatcacaca gagcaagacg tgcgttggtg
    14641 ccgcccacat caccgactaa tgcatacttt gtcattcttc aactgctccg ctaaagtcaa
    14701 aataattctt tctcacactg taaatacctg ggggcataac aacaacgccg gaaaggcagg
    14761 ctccctgtaa atatcgatct gggtcacaca attactttat cgtttcagca ccaattgcag
    14821 cgatgccttt ttgcaagctg ggcaaactaa gtatctgacc ccgcataagg aatagaacat
    14881 gctccatccg cgagccagaa ccatgttgtt attatcgctc cccgccgtgg caattgggat
    14941 tgcgtccagt cttattctga ttgtggtgat gaaaatcgcc tcggtattac agaatttgct
    15001 ctggcaacga ctgccgggaa ctctggggat agcccaggat tcacccctct ggatcatcgg
    15061 tgtattaacg ctaacgggta ttgcggtggg gttggttatc cgtttcagcc agggtcatgc
    15121 cggaccagac cccgcctgtg aaccgctgat cggcgcaccg gttccgccct ctgcgctacc
    15181 aggacttatc gtagcattaa ttctcggtct tgctggcggc gtcagcctgg ggccggaaca
    15241 tccgatcatg accgtcaata tcgcccttgc ggttgccatt ggcgctcgtc tgttaccgcg
    15301 cgtcaaccga atggagtgga ctattttagc ctctgccgga accatcggcg cgctgtttgg
    15361 tacacctgtt gcggcggcgt tgatattttc gcaaacctta aatggcagta gtgaagttcc
    15421 gctatgggat cgtctttttg cgccgttaat ggcggcagca gctggtgcac ttactaccgg
    15481 attatttttc catccccatt tttcactgcc cattgcgcat tacggacaga tggaaatgac
    15541 cgatattctc agcggtgcaa ttgtcgcggc gattgccatc gcagcaggga tggtcgccgt
    15601 atggtgctta ccacggttgc acgcgatgat gcatcaaatg aaaaatccgg tgctcgtgct
    15661 gggtattggc ggatttattc tcggtattct gggggttatt ggtggaccag tttcgctgtt
    15721 taaagggctg gatgagatgc agcagatggt ggcaaatcag gctttcagca ccagcgatta
    15781 ctttttgctg gcggtaatta aacttgccgc cctggtcgtt gctgccgcca gtggctttcg
    15841 cggtgggcga atcttcccgg cagtgtttgt cggcgtggca ttagggttga tgctgcatga
    15901 gcacgttccc gccgtaccag cggcaataac cgtttcctgc gctattctcg gcatcgtgct
    15961 ggtggtaaca cgcgatggct ggttaagtct ttttatggcg gcagtcgttg tacccaatac
    16021 cacattgcta ccgctgctct gtatcgtcat gcttccggca tggctgttat tagcaggtaa
    16081 gccgatgatg atggtcaatc gtccgaagca acagccaccc cacgataacg tttagcaaaa
    16141 aatgctctcc tttatgattt aagagttatg gcgatgatac ggggctttac ctccccgtaa
    16201 tattgcgtta acaggccgct gacggcttcg atcgtgaagg agaataacaa tgttcaggtc
    16261 actgtttctg gcggccgccc tgatggcatt taccccgctt gcagcaaacg caggcgaaat
    16321 caccctactg ccatcaatca aattacaaat tggcgatcgc gatcattacg gtaattactg
    16381 ggacggtggt cactggcgcg accgtgacta ctggcatcgc aattatgaat ggcgtaaaaa
    16441 ccgctggtgg cgtcatgata atggctacca tcgtggctgg gataagcgta aagcatatga
    16501 gcgtggctat cgtgaaggct ggcgcgatcg tgacgatcat cgcggaaaag gccgcggaca
    16561 tggacaccgc cattaaacac attcgccaat ggagcacaag gcctgatgcg acgctagtgc
    16621 gtcttatcag gcctacaaac cggctcttta acgcgtaggg cggataaggc gtttacgccg
    16681 catcgccaat agtgccagat gcgacgctca ttcaactaca atcccagcgc cgtccccacc
    16741 aataaccaga tattcagcgc cacgaccagc actacaatca cccagcctgt ctgttttacg
    16801 cgtttgctgt tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga
    16861 accagcgcca gggcgatacc aaaacttaac agcacctgac tcataaccag aatccgtgtc
    16921 ggatctaatc ccatcagaat gacaataaat gacggcagca tggtgactgt acgacgcacc
    16981 cacagcggga tatggaagcg aatgaacccc tgcatcacca cctgccctgc cagtgtcccc
    17041 accaccgttg aggacagtcc ggcagcaacc agacttaacc caaagaccgt tgccgcagca
    17101 tggcttaaca gcggttgcag cgtcaaataa gcctcatcaa gatcggcaac accagtatga
    17161 ccagaaaagt ggaacgccgc cgcagctgta gccatcatcg ccagattgac aaaaccggca
    17221 atagtcatgg cgatagccac atcccatttg gtggcggaat aacgttgttg acgcgaaccg
    17281 ccatgtaaat gctgagtgag cgaagagtgc aaataaatca catgcggcat aatcgtcgcc
    17341 cctaacacgc ctgctgccag gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt
    17401 cctttaccca gctgcgccag gttaggctgg gagaaaatca actcgacaat gtaagccgcg
    17461 gcaacaaaca acagtaaccc gccaatcact ttctccagcg gtttttgccc gcgacgttgc
    17521 agcattaaaa tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca
    17581 ccaagaatga gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc ggttgccatc
    17641 gcaataattt ctgcctgaac ccaatagaac cacactacgg gacgcggata gtgatcgcga
    17701 atctgctccg ccagattttt accggtggca atccctagtt tggcagagag gatctgaatc
    17761 agcatcgcca tcaggttggc ccaaacgaca acccacagta gctgatagcc gaagctggca
    17821 cccgcctgaa tattggtcgc aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac
    17881 gcaggtccca ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct actctcaacg
    17941 cgatagttcg tcatcttgtg cctctaaaac atagcctttg ctatgtttca tgctatgcca
    18001 aacgagaatg attatcaaat tcatttaaat gggttgtggt gatttctctg atagaccagg
    18061 attatgacta cgaaaagatt gatgacttga atgttatggt tatgtttaat gttagcacat
    18121 ttacataact ttcagcttcc atacacaaca tagcagaaat gtatgacagc tcactatttt
    18181 tgaagcttgt cacaggacgt cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt
    18241 tacatagaat gtgcacggaa atttaacctg cctcatattt ggagcaaata tggaccgcgt
    18301 ccttcatttt gtactggcac ttgccgttgt tgcgatcctc gcactgctgg taagcagcga
    18361 ccgcaaaaaa attcgtatcc gttatgttat tcaactgctt gttatcgaag tgttactggc
    18421 gtggttcttc ctgaactccg acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt
    18481 cgaaaaactg ctcggatttg ccaacgaagg gactaacttc gtctttggta gcatgaatga
    18541 tcaaggcctg gcattcttct tcctgaaagt gctgtgccca atcgtcttta tctctgcact
    18601 gatcggtatt ctccagcaca ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct
    18661 gctctccaaa gtcaacggca tgggcaaact ggaatccttt aacgccgtca gctccctgat
    18721 tctgggtcag tctgaaaact ttattgccta taaagatatc cttggcaaaa tctcccgtaa
    18781 ccgtatgtac accatggcag caacggcgat gtccaccgtt tctatgtcca tcgttggtgc
    18841 atacatgacc atgctggaac caaaatacgt cgttgcagcg ctggtactga acatgttcag
    18901 cacctttatc gtgctgtcgc tgatcaaccc ttaccgtgtt gatgcctgtg aagaaaacat
    18961 tcagatgtcc aacctgcacg aaggtcagag cttcttcgaa atgctgggtg aatacattct
    19021 ggcaggtttc aaagttgcca ttatcgttgc cgcaatgctg attggcttta tcgccctgat
    19081 cgccgcgctg aacgcactgt ttgctaccgt gactggctgg attggctaca gcatctcctt
    19141 ccagggcatc ctgggctaca tcttctatcc gattgcatgg gtgatgggtg ttccttccag
    19201 tgaagcactg caagtgggca gtatcatggc gaccaaactg gtttccaacg agttcgttgc
    19261 gatgatggat cttcagaaaa ttgcctctac actctctccg cgtgctgaag gcatcatctc
    19321 tgtgttcctg gtttccttcg ctaacttctc ttcaatcggg attatcgcag gtgcagttaa
    19381 aggcctgaat gaagagcaag gtaacgtggt ttctcgcttc ggtctgaagc tggtttacgg
    19441 ctctaccctg gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt aagaccatac
    19501 ataaaaaagc cggggataat tccccggctt ttttacgcct gttaatcagc taatggctgc
    19561 gggcgaccaa tcaaataccc ttgcagatat tgcaccccga gcttatgcaa tagcgcctgt
    19621 tgctgttgcg tctcgacaaa ctccgcgacc acactcaatg acttcgcttt cgccagatcg
    19681 gtaattgatc tcacaatcat cgcatccagc gtattcgtga caatatcttt cacaaagacg
    19741 ccatcaattt tgatgatatc agcctgcaaa cgctttaacc gttcgtagtt ggcatagccg
    19801 gtgccaaagt catcaatcgc aatccggaaa ccaaacttat gcagctgctc gatgttgtac
    19861 atgctgcttt ctgcgttaga aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc
    19921 ggggagatgt gataacgctt aaatagacga ataatccgcc cggcaatatt cttttgcagc
    19981 agcgtgagcg gcattaaatt aactgaaaag cgcgggcctt ttttgtcgca agggtgtgtt
    20041 gccagccact tcaacaagga ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac
    20101 tgagcaataa ggggcagaaa cttatccggg gtcataatgc cgccgtcata tttcagtcgc
    20161 gcgaggacct catcataacc ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc
    20221 aaatcaccct gatctaacgc cgtgcgaatg gtattcagca atagcacctg tttagtggtc
    20281 tgcccggaaa ccatctcctc tcggctatcc agcgccagca catgatgatg tgcgcaggat
    20341 tgctccgcca gccagcttaa ctgccccaac aagggttgca gggtttcctg atttccatca
    20401 aaacgccccc aggcagcacc atagcccata tccagcccgg tattgttcca gtgaatttgc
    20461 cgactattca ggatgttaac catatgctgg agtcgccctt ccgtttcagg cccgctcagc
    20521 actaacagca gttcactacc cggcaattga tacaactttt cgttttcctg catcaatggc
    20581 agcagcgtac ggtaaattga tcggatacag tgaacacgca tcatcaaccc gtaatgacga
    20641 ctcataaact caagattatc aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc
    20701 ggcgcttgtt ccaacgcacg aaagttgggc aggagcgtta acggatcggt cagcgcctgc
    20761 aaatgccagc ggcgattaag ccattcactg cgatgataaa tgcgcaccat atagagcagg
    20821 caaacgctaa aggaaatcag caccgcgaga ataaatgcca gcgaatattc ggtttccacc
    20881 ccttgcaaaa agttctggtt gtaattcagc aggcaaagcg ttgaaacagc ccaagtgaga
    20941 tttaaaaacg gatagcgaag cttaccgacc ccgagggtaa agatgatgaa gaaaacgggt
    21001 accaggtaac cggcaataaa gtcgttttca taaggtgtgc acaacagaag tagcagcacg
    21061 cttaacgctg ccagccagct taaggtaaat gcgcgtttct ctttgcccaa cgacggagcg
    21121 atatccctgc gccacaatat ctgcgcaaag tggggactta caatcatgcg agtgagatag
    21181 tagaaaagca tgttgtaaat cagcacagcg gtgaaaaggc ttagcaaatc aacgaccgtg
    21241 aaaatggcat ccgcatcgcc gaaaaaggtg gatatcttta gcgggaaatc aaagaagctc
    21301 cccacaagat acatgctgca tttgatgccg atcggcgtca ccaaaccaag ccagaacaga
    21361 cgttgccaga catatcggct ggtcaatcca taacgccaac gcgtccccag ctgccagcgt
    21421 aatattgcgc aggcacacaa cacgacaaac gtctggctac cgagcatgac cgcagtctgt
    21481 aaaaaagaga gatgaaagtt ccacagattg gtgcaaaaca tcccgcataa aacgggaacg
    21541 acaccgcgcc agccaaagat aaacaagatt gacagcatta cgcacagtgg catccacgcc
    21601 agaaagatat aactggaatg gataatggca agtggcgaaa taaaacggga tagctgaata
    21661 agtaccacgg tgagcgtaaa cgctagtgtg aatatcttga tattttttat caggttatgc
    21721 tccacaaaca tgagtacact actctcactg atcggggttt tcaggcacac aaataataag
    21781 tttattcgca ttgcgaagca aacaggggcc atcctgttgc cgatttttca tccaatgatg
    21841 ctttcaccag gcggcactaa cccggacgta gactgtatta caaaagcggc aaaaagcaga
    21901 gacaaaaaac ccccgctttg cagcgagggt tggaaatttg gtggagctaa gcgggatcga
    21961 accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag ccccacgatg
    22021 cgtttacgta ccaaatttgc tgggtgcaaa atttggtgga gctaagcggg atcgaaccgc
    22081 tgacctcttg catgccatgc aagcgctctc ccagctgagc tatagccccg tcacgtaaag
    22141 cttgtcgagt tgacgggcgg catcatatga attccgcccg aatgtgtcaa cggcaaattg
    22201 caacgtgtag tttcaatcgc tgaaaaatca ggcaaatgaa caattttgga aaaccgctcg
    22261 cattcagtag ttattcatgt cacggtttcc tgtaaagtgg tgctataaaa tgaactacta
    22321 atagacccac atacattcag ggaattgtta tgttcaagga gaggatgaca ccagatgaac
    22381 ttgccagact gaccggctat agccgccaga ccattaataa atgggtacgc aaggaaggct
    22441 ggacaacgtc accaaaacct ggcgtccagg gtggcaaagc ccgactggtt cacgtcaatg
    22501 aacaggttcg tgaatatatt cgtaatgctg aacgtccaga aggtcaggga gaagcgcctg
    22561 ccctttctgg tgatgcgccg cttgaagtat tgctggtgac actggcgaaa gagatgacgc
    22621 cagttgaaca aaaacagttt acatccttgc ttctgcggga agggattatc ggattgttac
    22681 aacgcttagg gattcgcgat agcaaataat atgaaaagat tacgcaataa aatgaccacc
    22741 gaagaactgg ctgaatgcct cggtgtggct aaacaaactg ttaaccgttg gatcagagaa
    22801 aaaggctgga aaacggaaaa atttcccggc gtgaaaggcg gtcgtgccag acttattctg
    22861 gtcgatacgc aagtttgcga gtttattcag aacacgccag ccttccataa cacaccgatg
    22921 cttctggaag ccgaagaacc ccttgccgaa tacgcaccgg ggattcgcac tcctgcttat
    22981 cggcaaatca tcagtgcaat agacaacatg actcacagtg agcaggaaaa agttgcgcaa
    23041 tttttgtcac gcgaaggaat tcgtaatttc cttgctcgtc tcgacataga tgaatcagct
    23101 tgacatcctc cacgccctga atgacgagga tccctgctac gttcaggctg tcgcctgaat
    23161 catttcggtg ggttcctgct tcaacgagcg gcctgactgc accatctctc cacaggcaag
    23221 aacggcgtgc cccgccgcta aaatgttacg agcgccgttt acatcggcgt tcgctgtata
    23281 tccacatacc tggcatctga atttactttg tgacaggcgg ttttctttcg ctgtatgacc
    23341 acagcacgcg caacgctggc ttgtgtacgc tggcggaaca gccag
//
LOCUS       DAZKIF010000077        23377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZKIF010000077 DAZKIF010000000
VERSION     DAZKIF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.077"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1236
                     /locus_tag="ACHOTW_002909"
     CDS             <1..1236
                     /locus_tag="ACHOTW_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP9844973.1"
                     /translation="NVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLIL
                     SLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYG
                     KDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGIN
                     YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYIS
                     RGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVK
                     DFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFT
                     ELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEAD
                     KALREAKSEGGNKVIIHHI"
     gene            1417..2907
                     /gene="dgcJ"
                     /locus_tag="ACHOTW_002910"
     CDS             1417..2907
                     /gene="dgcJ"
                     /locus_tag="ACHOTW_002910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP9844974.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2950..3453
                     /gene="yeaK"
                     /locus_tag="ACHOTW_002911"
     CDS             2950..3453
                     /gene="yeaK"
                     /locus_tag="ACHOTW_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP9844975.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3454..3558)
                     /gene="yoaI"
                     /locus_tag="ACHOTW_002912"
     CDS             complement(3454..3558)
                     /gene="yoaI"
                     /locus_tag="ACHOTW_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP9844976.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3728..4174
                     /gene="yeaL"
                     /locus_tag="ACHOTW_002913"
     CDS             3728..4174
                     /gene="yeaL"
                     /locus_tag="ACHOTW_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9844977.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(4131..4952)
                     /gene="nimR"
                     /locus_tag="ACHOTW_002914"
     CDS             complement(4131..4952)
                     /gene="nimR"
                     /locus_tag="ACHOTW_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP9844978.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            5049..6230
                     /gene="nimT"
                     /locus_tag="ACHOTW_002915"
     CDS             5049..6230
                     /gene="nimT"
                     /locus_tag="ACHOTW_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP9844979.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6285..6632
                     /gene="yeaO"
                     /locus_tag="ACHOTW_002916"
     CDS             6285..6632
                     /gene="yeaO"
                     /locus_tag="ACHOTW_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9844980.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6654..6908)
                     /gene="yoaF"
                     /locus_tag="ACHOTW_002917"
     CDS             complement(6654..6908)
                     /gene="yoaF"
                     /locus_tag="ACHOTW_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP9844981.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            7091..8116
                     /gene="dgcP"
                     /locus_tag="ACHOTW_002918"
                     /pseudo
     CDS             7091..8116
                     /gene="dgcP"
                     /locus_tag="ACHOTW_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
     gene            8149..8247
                     /gene="yoaK"
                     /locus_tag="ACHOTW_002919"
     CDS             8149..8247
                     /gene="yoaK"
                     /locus_tag="ACHOTW_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP9844982.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8250..8324
                     /gene="yoaJ"
                     /locus_tag="ACHOTW_002920"
     CDS             8250..8324
                     /gene="yoaJ"
                     /locus_tag="ACHOTW_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP9844983.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8383..8631)
                     /gene="yeaQ"
                     /locus_tag="ACHOTW_002921"
     CDS             complement(8383..8631)
                     /gene="yeaQ"
                     /locus_tag="ACHOTW_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9844984.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8778..8960)
                     /gene="yoaG"
                     /locus_tag="ACHOTW_002922"
     CDS             complement(8778..8960)
                     /gene="yoaG"
                     /locus_tag="ACHOTW_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP9844985.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8964..9323)
                     /gene="yeaR"
                     /locus_tag="ACHOTW_002923"
     CDS             complement(8964..9323)
                     /gene="yeaR"
                     /locus_tag="ACHOTW_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP9844986.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9496..10134)
                     /gene="leuE"
                     /locus_tag="ACHOTW_002924"
     CDS             complement(9496..10134)
                     /gene="leuE"
                     /locus_tag="ACHOTW_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP9844987.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10261..11184)
                     /gene="dmlR"
                     /locus_tag="ACHOTW_002925"
     CDS             complement(10261..11184)
                     /gene="dmlR"
                     /locus_tag="ACHOTW_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP9844988.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11287..12372
                     /gene="dmlA"
                     /locus_tag="ACHOTW_002926"
     CDS             11287..12372
                     /gene="dmlA"
                     /locus_tag="ACHOTW_002926"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP9844989.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12623..14233
                     /gene="yeaV"
                     /locus_tag="ACHOTW_002927"
     CDS             12623..14233
                     /gene="yeaV"
                     /locus_tag="ACHOTW_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFP9844990.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14265..15389
                     /gene="yeaW"
                     /locus_tag="ACHOTW_002928"
     CDS             14265..15389
                     /gene="yeaW"
                     /locus_tag="ACHOTW_002928"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP9844991.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15445..16410
                     /gene="yeaX"
                     /locus_tag="ACHOTW_002929"
     CDS             15445..16410
                     /gene="yeaX"
                     /locus_tag="ACHOTW_002929"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP9844992.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHQSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16464..17579)
                     /gene="rnd"
                     /locus_tag="ACHOTW_002930"
     CDS             complement(16464..17579)
                     /gene="rnd"
                     /locus_tag="ACHOTW_002930"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP9844993.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17661..19346)
                     /gene="fadD"
                     /locus_tag="ACHOTW_002931"
     CDS             complement(17661..19346)
                     /gene="fadD"
                     /locus_tag="ACHOTW_002931"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP9844994.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19551..20132)
                     /gene="yeaY"
                     /locus_tag="ACHOTW_002932"
     CDS             complement(19551..20132)
                     /gene="yeaY"
                     /locus_tag="ACHOTW_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP9844995.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(20172..20867)
                     /gene="tsaB"
                     /locus_tag="ACHOTW_002933"
     CDS             complement(20172..20867)
                     /gene="tsaB"
                     /locus_tag="ACHOTW_002933"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9844996.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20925..22835)
                     /gene="yoaA"
                     /locus_tag="ACHOTW_002934"
     CDS             complement(20925..22835)
                     /gene="yoaA"
                     /locus_tag="ACHOTW_002934"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9844997.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22967..23311
                     /gene="yoaB"
                     /locus_tag="ACHOTW_002935"
     CDS             22967..23311
                     /gene="yoaB"
                     /locus_tag="ACHOTW_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9844998.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5726 a         5959 c         5675 g         6017 t
ORIGIN      
        1 aatgtaaaac tcatcccaca gatgaattac ctgatggttg ttgtggcttt gtttttcctt
       61 aacgccgtca tttttctttt catgttaatg aaatatttca ctaacaaaca aattttacca
      121 acactcattt taagccttgc atttttaagt ggccttatct atttagttga aaccattgta
      181 attatccata aaccaattaa cggcagtaca ctgatccaga caaagtcgaa tgatgtttct
      241 attttctata ttttccgcca actcagtttt atttgtttaa cctcgctggc gctcttttgt
      301 tatggaaaag acaacatcct tgacaacaat aagaaaaaaa ctggcattct gttgctggcg
      361 ctgatccctt ttttagtttt tccccttctg gcacacaatc tgagcagtta taacgctgac
      421 tattctttgt atgtcgtcga ttactgtccg gacaatcata ctgcgacctg gggaatcaac
      481 tatacaaaaa tattggtttg tctgtgggca tttttactgt tctttattat catgcgtaca
      541 cgattagcca gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc
      601 aacttacttc tactgactct ggatgagtat aattatacca tctggtatat cagtcgcggg
      661 attgaagtct ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcaggag
      721 ctgcaactct ccagcaaact ggcagttcat gatgtgctga ccaatattta taatcggcgc
      781 tactttttca acagcgtaga gtcattattg tcgcgacctg ttgttaagga cttctgtgtc
      841 atgctggttg atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt
      901 gataaagtgc tggtttcaat tgtcgatatt atccagcaaa gcatccgccc cgatgatatt
      961 ttagcgcgac tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag
     1021 gcaaaaatca ttgcggaacg tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac
     1081 agatatgatg ttcctgaaca aatgaccatc agtattggca cagttttttc aacgggtgac
     1141 acgcgtaata tctcgcttgt catgacggaa gcagataaag ccttacgcga agcgaaaagc
     1201 gaggggggca acaaagtgat tattcatcat atttaagtgc aaaaatattc agagccatgc
     1261 tttttgcgtg gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc
     1321 ggcgcagaga tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt
     1381 tttctcgttt cactaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc
     1441 cggcatttta tcgccgcaag tgtcattgtg ctgacatctt ccttccttat ttttgaactt
     1501 gtcgccagcg acagagcaat gagtgcctat ctgcgctata tcgtgcagaa agcagactcc
     1561 tcctttcttt atgataagta tcagaatcag agtattgccg cgcatgtgat gcgcgctctc
     1621 gctgctgagc agtcggaagt gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag
     1681 tctgccaata atacccatgg cttaaacctg actgcccata aatacccggg cttacgcggc
     1741 acattacaaa ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc
     1801 gcttttgatc aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg
     1861 atggccgaag agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat
     1921 gagccggtta atgttgaata ctttgcgatg aataactggt ccatcctgca gtcaggaagt
     1981 attggcatcg atcgcaaaga tattgaaaag gtatttaccg ggcgtaccgt attgtcgagc
     2041 atttaccagg atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtgtatgtt
     2101 gcagggcagc taaaagggat tgtgctgctg gatattaaca aaaacaatct gcggaatatc
     2161 ttttacactc atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgataca
     2221 gattcggggc gcgacattat catcaaccag agcgaagata atctgttcca gtatgtcagt
     2281 tacgtccatg acttaccggg cggcattcgt gtctcgttat ccattgatat tctttacttt
     2341 atcacgtctt cgtggaaaag cgttctgttc tggattttga cggcgttaat tttgctgaat
     2401 atggtgcgga tgcacttccg cttataccaa aatgtgtcgc gagaaaatat tagtgatgcg
     2461 atgactggac tgtataatcg caaaatttta acccctgaac tggagcagcg gttgcagaaa
     2521 ctggtgcaat ccggttcttc ggtgatgttt attgcaattg acatggacaa gttaaagcta
     2581 ataaatgaca ccctcggtca tcaggaaggg gatttagcga ttacgttgtt agctcaggcg
     2641 attaaacaat cgattcgtaa aagtgattat gccatccgac tcggtggcga tgaattctgc
     2701 atcattcttg tcgattcgac gccgcaaatt gcagcacaac tgcctgaacg tatcgaaaaa
     2761 cgtctgcaac atatcgcgcc gcagaaagag atcggcttct cttccggtat ttacgcgatg
     2821 aaagaaaacg atacgttaca tgatgcgtat aaagcttccg atgagcgttt atatgtcaat
     2881 aagcagaaca aaaacagccg ttcatgataa ccttctgtgg ttgtttgctt gtaatctcag
     2941 gagcgtgaaa tgactgaaat ggctaaagga agcgtgacgc atcagcggtt aatcgcttta
     3001 ttatcacaag aaggtgctga tttccgcgtc gttacccatg aagcggtagg taaatgtgaa
     3061 gcggtgtcag aaattcgtgg caccgctcta ggccagggtg ctaaggcact ggtatgtaaa
     3121 gtcaaaggca atggcgtaaa tcaacatgtt ctggcgatcc tcgcagccga tcaacaggcc
     3181 gatctgagcc aactcgccag ccatataggc ggattacgcg cttcgttggc cagcccggcg
     3241 gaagtcgatg aactcacagg ctgtgtcttc ggcgcgatcc cccctttcag cttccatcca
     3301 aaactcaaac tggttgccga ccctctactc ttcgaacgat ttgatgaaat cgccttcaat
     3361 gcagggatgc tggataaatc cgttattttg aaaaccgcgg attatttgcg cattgcgcaa
     3421 ccagaactgg tcaatttccg ccgcactgcg taactagcct gtccgttcaa taagcagaac
     3481 ggacaaaacc agcacaacag caatggcaaa aaacgatgac gtgataatca gtgtctcgac
     3541 gaacatttga tcgttcatag catacccccg ggagatagct gttttcacgt tattgttagc
     3601 gtgcacaaat ggcagtttga tgacagttcg cctgattttt tatgcaaaaa acgtaaagat
     3661 tttctacttc cttcctgcag caagcgtaaa gtaagcaggc ttattatttt ttggcaagga
     3721 aaccacgatg tttgatgtca ctttgctgat cctgctcggg ttagctgcgc tgggctttat
     3781 cagtcataac accactgtcg ccgtttcaat tctggtgttg atcattgtcc gcgtcacacc
     3841 gttaagcacc tttttcccgt ggattgaaaa acaggggcta agtatcggga taatcattct
     3901 gacgattggc gttatggccc ccatcgccag cgggacgcta ccgccctcaa ccttgattca
     3961 ctcgtttctg aactggaaat cgctggtggc aattgcagta ggcgttattg tctcctggct
     4021 gggtgggcgc ggcgtgacgt taatgggcag ccagccgcaa ctggttgctg ggctgctggt
     4081 gggcactgtt ttaggcgtag cgctgtttcg cggcgtaccg gttggcccgc ttattgccac
     4141 gggtctggtt tcgctgattg tggggaaaca gtagttaatc ccgcaatata tcggcctggc
     4201 gtttgcccca ggcctttctt aaacatagta ataaaggccg ttgttgaatc ataccccagc
     4261 gtatgagcaa ctttctgtac agtatcgcct ttcactaatc cctgaagcgc cataatcagc
     4321 tgtaactgct gtcgccactg gcgaaaactc aaccccgtct cttttactat cagtcgggca
     4381 agattgcgtt cactcatcgc aaaaaagcca gcccattgcc ccaatgcccc ccactcgacg
     4441 ggctctttcg ccatcatctc caccatcgcg cggattttag gatgagaaga aacgggtagg
     4501 tgcagctgtt gttgtggctg ctgagggagt tcatcaaaca ggacctgaat taatctgcgg
     4561 gtcatcggct cagcacgctg agtcgtcgtc ctgtttgcca gagttaaaat taattcccgg
     4621 cataagggcg atattttcaa tgtgtaacat gttgtcggca ttgtcactgc gctgggttcg
     4681 ataaacagaa aacagagttc agcgttagct gtcacttgat tgctatgttc caccccgccc
     4741 ggtatccaaa ccgcatattg tggcggcacc atccacaaag cattttccac cgtacaggta
     4801 attgcaccat gtagcgccag tatcagctgt cctttgcggt gctgatgtac aggactggtt
     4861 aactcatccg ttccggcgtg aatacaaaat gcgaccgcgg cttcatggtg tcggtcgggt
     4921 tcatagccat tgagattcaa cctgtgcatc attttgtccg aatttagcga taatttgtca
     4981 ttttagcttg attcaacata acaataaaaa cggtaaggta cagcctcgtt tgtaacaatg
     5041 agaagcatat gacctgttca acttcattaa gcggcaaaaa caggattgtc cttatcgctg
     5101 gcattctgat gattgccact acattacgcg tcaccttcac cggcgcagca ccgttactcg
     5161 atacgattcg ttccgcttac tcgctgacga ctgcgcaaac cggcttattg accaccctgc
     5221 cattattggc ctttgcgcta atctcacctc tggctgcccc ggtggctcga cgttttggta
     5281 tggaacgtag cctgtttgcc gcgttacttt tgatctgtgc aggtatcgca attcgctctc
     5341 tcccttcgcc ttacttatta tttggcggta cagcggtcat tggcggtggg attgcattag
     5401 gtaatgtctt actgccagga ttaattaaac gcgatttccc tcattccgtc gccagactta
     5461 ccggcgcata ttccctgaca atgggagctg cggcggctct gggatcggct atggtagtgc
     5521 cgctggcttt gaacggtttt ggctggcaag gcgcgttgct catgctgatg tgttttcctc
     5581 tgctggctct ttttttatgg ctgccacagt ggcgaagcca acaacatgca aatttgagta
     5641 cttcacgcgc cttacatact cggggtatct ggcgttcgcc gcttgcctgg caggtaacat
     5701 tgtttcttgg gatcaactca ctggtctatt acgtgattat tggctggctt ccggcgatcc
     5761 tcatcagtca cggctatagc gaagcacagg cgggttcact gcatggtttg ctgcaactag
     5821 ccacagcagc tcccggtttg ctgatcccac ttttcttaca tcatgtgaaa gaccagcgtg
     5881 gtattgcagc gttcgttgcc ttgatgtgcg cagtgggcgc ggttgggctc tgcttcatgc
     5941 cagcgcacgc gatcacctgg actctgcttt tcggttttgg ttccggcgca acaatgatac
     6001 tggggttgac gttcattggt ctgcgggcta gttctgcgca tcaggcggcg gcactctcgg
     6061 ggatggcaca atccgtcggg tatttgttgg cagcctgtgg gccgccgctg atgggtaaaa
     6121 tacacgatgc taacggtgac tggtccgtac cactaatggg tgttgcaata ctttcactac
     6181 tgatggcgat tttcggactt tgtgccggga gagacaaaga aattcgctaa tatccggtac
     6241 tatagtgacg tgacaaatca tgcgtgaaag ggagaacaaa cacgatgaat attcagtgca
     6301 aacgcgttta tgatccggca gaacagagcg atggttatcg cgtactggtc gaccgcctct
     6361 ggccacgcgg tatcaaaaaa accgatttag cccttgatga gtgggataaa gaaatcacgc
     6421 cgtcaacgga actgcgcaaa gcctttcacg gcgaagtcgt cgattttgca acctttcgcg
     6481 agcaatatct tgcagaactg gcacaacacg agcaagaagg aaagcggctg gcggacatcg
     6541 ccaaaaaaca gccgctgacc ctgctctact cagcaaaaaa caccacgcag aaccatgcgc
     6601 tggtgctggc cgactggcta cgtagcttgt gattttagta cagcatccgg cggttatttt
     6661 tcaccagccg gatggtccag tcgccacaat gcccattcat caatcgtttc accgcccggt
     6721 aatttgcaat tgttgctgac cccttgcgct gtctgcactg gaatgagcgt cccgcccttc
     6781 tgctggcaat agaccgacgc cggatttgcc ataccaatct gcggcggttt aggtgcttct
     6841 ggctgagaag gggttgaaca accagccagg accagcaagc aaggcagaac aaaactgata
     6901 attttcattt attgatctca catatgtatc caagcttaga gtatcgcggt atcgttttgt
     6961 tttgcagcac tatttttatt acattcactc aaaacatatt acgtcttgtt tcatctttgt
     7021 tgatgatgtt ttatcatgcc tgcaaagatt aaataatcag catttacccg ccgtatcctg
     7081 gagttgtttc gtgtcagatc agattatcgc ccgcatctcg caatcccttg ccaaagaaca
     7141 gtcactggaa agtctggtcc gacagcttct ggagatgctg gaaatggtca ctgatatgga
     7201 atcaacctac ctgaccaaag tggatgtcga agcgcgcctg cagcatatta tgtttgcccg
     7261 taacagccag aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt actcgttatg
     7321 caaacgcgcc attgatgaaa actgcttttt cagcgatgaa gtccctgacc gttggggcga
     7381 ctgtattgcg gcacgcaatc ttggcatcac cacatttctg agcacgccaa ttcacttacc
     7441 ggatggatca ttctatggca cgctttgcgc cgccagcagt gagaagcgcc agtggagtga
     7501 acgcgcggaa caggttttac agttattcgc cggactgatt gcacaatata ttcaaaaaga
     7561 ggcactggtt gaacagctgc gcgaagccaa tgctgcgctg attgcgcaat cgtataccga
     7621 ctcgttaacc gggctaccga atcggcgggc gatttttgaa aatctgacga cgctgttttc
     7681 cctcgcccgg catcttaacc aaaagataat gatcgcgttt atcgatctgg ataacttcaa
     7741 attaatcaat gatcgttttg gtcataatag tggcgatctg tttctcattc aggttggcga
     7801 gcgccttaat acgctccagc aaaatggcga agttattggt cgtctcggcg gtgatgagtt
     7861 tttggttgtt tcactaaaca acgagaatgc ggatatttcg tcgctgcgag aacgcattca
     7921 gcagcaaata cgcggataat atcacttagg tgatgttgat ttgtattatc ctggtgccag
     7981 tcttggcata gtagaagtcg atcctgaaac gaccgatgca gacagtgccc tacatgctgc
     8041 cgatatcgcg atgtatcagg agaaaaaaca caaacagaaa acaccttttg tcgcgcattc
     8101 agcgccacat tcctgaggcg tattcacatc cttttgattg gtgataacat gcgaatcggt
     8161 attatttttc cggttgtaat cttcattaca gcggtcgtat ttttagcatg gttttttatt
     8221 ggcggctatg ctgccccggg agcataaaga tgaaaaaaac aacgatcatt atgatgggtg
     8281 tggcgattat tgtcgtactc ggcactgagc tgggatggtg gtaacgtcac atctaaaaaa
     8341 tagcaaaggc tgcctgtgtg cagcctttgt gcaatttacg cgttaacttt taatcttcct
     8401 gtagataaat agcacgacaa tcgcaccaat aacggcaacc acgaagctgc caaaattgaa
     8461 gccatcgact ttaccaaagc caaacagcgt gctgatccaa ccgccgacta cggcaccgac
     8521 tatccccagc aggatagtca taaagaatcc accaccatct ttacctggca tgatccactt
     8581 cgccagaata ccggcaataa gcccaaaaat aatccatgac agaatgccca ttgtttcctc
     8641 acttatctgt tttgcattag cgggttagtc gctgataaaa agcatagcac aacatcggga
     8701 gggcaagatt tgtgacgagc atcacggagg tttttttgcg atggcgcaga aattgcgcca
     8761 tcaacgatca gtgataatta ccaaccacaa acatcatgtt cgttttccgt gtcataagaa
     8821 cgcacggtat tcaccagatc ttttatcact tcagccgcca cttctggcac cagcaaagtc
     8881 atcggcgtct ctgtttcata atcgacagaa acgccattgc tgttattggt gacggtcacg
     8941 gtatacgttg ctttgcccat gattcatttc ccgttatgaa tgactttccg ttgttgcgca
     9001 ccttccatca ggacttcagg agccacgaag aagtcaatgt tgaaataagt atcgtcagtc
     9061 atggcttcaa tgttgtgcca cttttctgga gggaacaccg caaactgccc cgcttcgata
     9121 aggatcacct gatcaggctc tgcactgtgt tcatcagcgt agccgagata tttgaccgcc
     9181 ccatgcataa cggaaaggcg tgggtaaacc cccgggcgcg ttcctttatc aagatgacgt
     9241 tcgaatattc cggcaggcgc agtttgttta ttccagaaag gcgttgagcg cgtatgaata
     9301 taattctgtg ggatttgcag catccttttc cctccttcgg tgaatgcgct gaaaacggtt
     9361 tattccagcc gtttcagggt acgcctgata atttgctttt taaataccat ttattggtta
     9421 ctttttagca ccatatcaga gaaaaatcag ggaggattat agatgggaag cccgcgacaa
     9481 cgcgggctga aagcatcagg attgcagcgt cgccagtcgg gcagcgaaac ccacgaacat
     9541 caaaccaatc agtgagttgc ccactttagc cagtttcttt ttggtacgta tgtactgcgt
     9601 gacaaaagca ccagatataa tcaggaagct caaatagcag aaactcacca gttccagcgt
     9661 cgtcgccaga ataaagaatg aaattcccgt atgtggggca ttaacatcga taaactgtac
     9721 gaaaaacgac acatagaaca aaatggcttt cggattagtc aggctcaaaa ttaacgcgcg
     9781 tttaaaaata gcaccgtatt ggggctcatc ggatttggcc tcgttatttt tacccttcag
     9841 ggtcgcgtaa agaattttac tccccagata gagcaaataa aacgcaccaa gataacgtac
     9901 aatgttgaat aatatcgggg tggtcttaat taatgtcgcc attccagccc atgccagaaa
     9961 catcaatacc gcatcgccaa taaatacacc gcaggccgca agataaccgc ctttcatacc
    10021 gctactgacg ctatttttga gtacaaacag ggtatttggc cctggcacca acacaataaa
    10081 aatggcccca accagatagg tccagtaatt cagaaccccg tattcagcga acacattaac
    10141 ctctttaatt atctttcgat catgcgcgat taaaggtgaa tatgctaacc aatctgtagc
    10201 ggcttagaaa ggagaaaatc aggttttaac ctgatatcaa cccgataatt gaatcattaa
    10261 tcaggcatgc tccagtgaaa aattcgggta gtgctcggca aaatactggc gtaaaaattc
    10321 taccgttatc cgcactttcg ctgacgtcgc cagccttgaa acataaacgg cccagacgtt
    10381 cgctggctgg taatattccg gtagcacttg cactaaatga ccactggcaa tgttttcgct
    10441 aacatcccac caggaacgca gagcaatccc ctgcccatcg agacaccact gatggacaat
    10501 ttctccatga ttagacgaca gtgggccggt gactttaatc gcatggtggc cttctttatt
    10561 gcgtaattgc cagacaccaa aaggatgatc gcgctcttta atgaccaggc acggcaatgc
    10621 agaaagatcg gttaaatgtt tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca
    10681 gagaatgcgg taattggtcg ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc
    10741 aatgcgaata tcgagatcga caccttcatt gaccaaatcg accagccggt cttccacatc
    10801 aaaacgtaat tccagttgcg gatacgcctt cgccagcgct gataacgccg gagccaccac
    10861 ctgtcgtcca aaaccaaagc tgctgataat acgcagcatc ccctgtggca cctgacgcac
    10921 gtcagaaagt tcgtccatca tctgaccgac atcctgcaaa atccgctgcg cccattcata
    10981 aatccgctct ccttcttcgg taatggtgac gcggcgggtg gtgcggtgta acaacaccac
    11041 gtttagcgtt tgctccagca aggcgatgcg cttgctgacg aacgccggtg aaacgcccag
    11101 ttcttccgcc acggcggcaa aaccagcccg gcgagccacc agcataaaga cgcgcaaatc
    11161 attcagcagc ggtaaattat tcatgattcg tgttttatgt ttcaccagtt acggggatta
    11221 attccttttc agtcaattat aggatggtga tgttgtcaat tttgatggtc aggaagtgag
    11281 aacccaatga tgaaaacgat gcgtattgct gcgatcccgg gagacgggat tggcaaagaa
    11341 gtccttcctg aagggattcg cgtgttacag gctgccgctg agcgctgggg cttcgccttg
    11401 agttttgagc aaatggagtg ggcgagctgc gagtattaca gccatcacgg taaaatgatg
    11461 ccggacgact ggcatgagca acttagccgt ttcgacgcca tctattttgg tgccgtcggc
    11521 tggccggaca ccgttccgga ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt
    11581 gaattcgacc agtacgtcaa cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg
    11641 ctggcgggaa aacagcctgg cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc
    11701 gaatattcct cgctcggcgg tagagtgaat gaaggtacag agcatgaagt cgtcattcag
    11761 gaatcggtat ttacgcgtcg tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg
    11821 caaagccgcc cacgtaagac gctaacttct gccactaaat caaacggttt agccatcagc
    11881 atgccgtact gggatgagcg agtggaagca atggccgaga attacccgga gatccgctgg
    11941 gacaagcagc atattgatat tctctgcgcg cgttttgtga tgcagccgga acgcttcgat
    12001 gtggtggtgg cgtccaattt gtttggcgat atcctttccg atcttggccc ggcctgcacc
    12061 ggcaccattg gcattgcccc atccgccaac ctgaatccgg aacgcacttt cccctcgctc
    12121 ttcgagcctg tccacggttc cgcgccggat atctacggga aaaatattgc taaccctatc
    12181 gccacaattt gggccggggc aatgatgctc gattttctcg gcaatggcga tgagcgtttc
    12241 cagcaagcgc ataacggtat tctggcagcg attgaagaag tgattgctca cgggccgaaa
    12301 acaccggata tgaaaggcag tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt
    12361 attttgcgtt aaggtcaaac cagtttattt gaaccgcgtc actgacgcgg ttttgttatt
    12421 cgttctttgc agttaataac ctgcgtcatt tcacctttta ttgtttccgt ttcgtgtttt
    12481 atggctttcc gtattcttaa ttgtttaatt tatgtaacat gcaaataatc aattcgttaa
    12541 acacgacata caactcatta ccctgcaaat aattacaact ggcaaaagcc agagacgggc
    12601 ttttatgccc aggagatgac tcatgatgag caatgttaag aaaaaagatg tgccgctgat
    12661 aagcatcagc ctggtggcca ttcttttcat cgcagctgca ttaagccttt tcccacaaca
    12721 atcggccgac gcggccaacg caatatacac ttttgttacg cgtacgttag gttccgcagt
    12781 acaggtattg gttttgctgg caatgggact agtgatttat ttagccacca gtaaatacgg
    12841 caatattcgt cttggcgaag gaaaaccgga atacagcacg ctctcctggc tgtttatgtt
    12901 tatttgtgcc ggtttaggtt cttctacgct ttattggggg gttgctgaat gggcctatta
    12961 ttatcaaacg cctggattaa atatcgcacc gcgttcacaa caggcactcg aatttagcgt
    13021 cccttactcc ttcttccact ggggcatcag cgcctgggca acttatacgc tggcctcatt
    13081 aatcatggct tatcactttc atgtgcggaa aaacaaaggt ctgagtcttt ccggcattat
    13141 tgccgcgatt accggcgttc gcccgcaagg cccatgggga aaactggttg atttgatgtt
    13201 cctgatcgcc actgtcggcg cactgactat ttcccttgtt gtcaccgcag caacctttac
    13261 tcgtggactt tccgcactga ccggtttacc cgataacttc accgtgcagg catttgtgat
    13321 cctgctttcc ggcggcattt tttgcctaag ctcatggatt ggtatcaaca acggtttgca
    13381 acgtctgagc aaaatggttg gctggggcgc gttcctgctg ccattactgg tgctgattgt
    13441 cggtccaacc gaatttatta ccaacagcat catcaatgcc atcggcctga ccacgcaaaa
    13501 cttcctgcaa atgagcttat tcaccgatcc gcttggcgat ggttcattta cccgcaactg
    13561 gaccgttttc tactggctgt ggtggatctc atacacccct ggcgtagcaa tgtttgtcac
    13621 ccgcgtttcc cgcggtcgta agattaaaga agttatctgg ggactgatcc tcggcagcac
    13681 cgtcggttgc tggttcttct tcggcgtaat ggaaagctat gccattcatc agtttatcaa
    13741 tggcgtaatc aacgtcccac aggtgctgga aactctgggc ggcgaaacag ctgtacagca
    13801 agttctgatg tcgttgccag ccggtaaatt gttcctcgcc gcatacctgg gcgtgatgat
    13861 tattttcctt gcctcgcata tggatgcagt ggcctacacc atggcggcga ccagtacgcg
    13921 taatctccag gaaggtgacg atcctgaccg tgggctgcgt cttttctggt gcgtggtgat
    13981 cactctgatc ccgctttcca tcttgtttac cggtgcttcg ctggaaacga tgaaaaccac
    14041 cgtcgtgctc acagcccttc ccttcctcgt cattttactg gtgaaagtcg gcggatttat
    14101 tcgctggctg aaacaggatt acgccgacat tccggctcat caagttgaac attatctccc
    14161 gcagacaccg gttgaagccc tggaaaaaac accagtgctc cctgcgggaa ccgtattcaa
    14221 aggcgacaac tgagcgccat catcctaacg ataaaggtat ccctatgagc aatctgagcc
    14281 ctgactttgt actacccgaa aatttttgcg ctaacccgca agaggcgtgg accattcctg
    14341 cccgttttta taccgatcag aacgcctttg aacacgaaaa agagaacgtc ttcgccaaaa
    14401 gctggatttg cgtcgctcac agcagcgaac tggcgaatgc caatgattat gtgacgcgtg
    14461 agatcattgg cgaaagcatc gtgctggtac gcggtcgtga taaggttttg cgcgcgttct
    14521 ataacgtgtg tccgcaccgt ggtcatcagt tgttgagcgg tgaaggaaaa gcaaaaaatg
    14581 tgattacctg cccatatcac gcatgggcat tcaaactcga tggcaatctg gcccatgcac
    14641 gtaactgcga aaacgtcgcc aatttcgata gcgacaaagc gcaactggtt ccggtgcgtc
    14701 tggaagaata tgccggattc gtcttcatca acatggaccc caacgccacc agcgtagaag
    14761 atcagttacc cggtctgggc gcgaaagtgc tggaagcctg cccggaagtc cacgatctga
    14821 aactggcggc ccgctttacc acccgcacgc ctgccaactg gaagaacatt gtcgataact
    14881 atctcgagtg ctatcactgt ggtccggcgc atccaggttt ctccgactcc gtacaggttg
    14941 atcgttactg gcacaccatg cacggtaact ggacgctgca atacggtttc gccaaaccgt
    15001 ccgaacagtc gtttaaattt gaagagggta cggatgcggc attccacggt ttctggctgt
    15061 ggccgtgcac gatgctgaat gtcaccccga tcaaagggat gatgacggtc atttatgaat
    15121 tcccggtgga ttctgaaact accctgcaaa actacgatat ttacttcacc aatgaagagt
    15181 taaccgacga gcaaaaatcg ctgattgagt ggtatcgcga tgtgttccgt ccggaagatt
    15241 tacgtctggt tgaaagcgta cagaaagggc tgaaatcgcg tggctatcgt ggtcaggggc
    15301 gcatcatggc cgacagtagc ggtagcggca tttccgaaca tggtatcgcc catttccata
    15361 atctgctggc gcaggtattt aaggactaat gacatcggcg gcggtatttt ccgccgccgg
    15421 gctgattttt gatggagtac agcaatgtca gactatcaaa tgtttgaagt acaggtgagt
    15481 caggtggaac cgcttaccga acaggtgaaa cgcttcacgc cggtggcaac cgatggcaaa
    15541 ccgctgcctg cgttcaccgg aggaagtcac atcatagtgc agatgagcga tggtgataac
    15601 cagtacagta atgcgtattc actactgagt tcaccgcata acacctctag ttatcagatt
    15661 gccgttcgac tggaagaaaa ctcgcgcggc ggttcccgct ttttgcatca gcaggtaaaa
    15721 gtgggcgatc ggttaacgat ctcaacgcct aataacctgt ttgcgctaat tccctcagcc
    15781 agaaagcatc tgtttatcgc gggcggtatt ggtatccccc ctttcctgtc gcacatggca
    15841 gagttgcaac acagcgacgt cgactggcag ctacattact gctcgcgaaa tccagaaagt
    15901 tgcgcatttc gtgatgagct agtccagcat ccgcaggctg agaaagtcca tttgcatcaa
    15961 tcatcgaccg gaacacgact ggaattagcg cgattattgg cggatatcga acctggcaca
    16021 cacgtttata cctgtggccc cgaggcgcta attgaagcgg taagaagtga agctgcgcgt
    16081 ctggacatcg ctgccgatac gctgcacttt gagcaatttg ctatcgaaga caaaaccggc
    16141 gatgcattta ccctggtgct tgcccgttcc ggaaaagagt ttgtggtgcc ggaagagatg
    16201 actattttgc aggttattga aaataataaa gccgcgaaag tggaatgttt atgtcgtgaa
    16261 ggggtatgcg gaacctgcga aacggcaata ctggaaggtg aagctgacca tcgggatcag
    16321 tattttagcg atgaagagcg tgccagccag caaagtatgt tgatctgttg ttcgcgtgcg
    16381 aagggtaaac gcctggtgtt ggatttgtag tttgccgaag ccggatgtgg cgctgagcgc
    16441 gcccagtccg gcttcggaaa attttactgc ggatattcct gcaataaatt gtgtaatgct
    16501 tccgccatca gctcaccacg ccagccggaa atcagctccg gcaaattgtt ctgcggtttc
    16561 agtttccagt gccagttcag cagttggttg atttgccgac gcgatgccag caattcggcg
    16621 ctgatcttgt gcgtttcgct cacgtcagta atcagcgact taatcgcttt aaacgcttta
    16681 cgataacccg gcatgtccat caggttaagc atcggctgcg gtaaggcctc ttccggcaat
    16741 gtctgcgctt tttccaccag cgccagcagc gttttaccgt gaaagcggat ttcgctaccg
    16801 gataaaccca ggctgtccag ttcgcctaaa cttcccggca tataacgcgc taccgaccac
    16861 aaatgctctt cacgcacgac aaagttcacc gccagatcgc gctctcgcgc cttgcgcagt
    16921 cgccagtcgg ctaacagttg cagacaggcc agttggcgtg tgcgtaattg ccaggcattg
    16981 gtgatatcac gccaggcatc ttccggcgca acgacttcct gacgacgcat ttgcatcagg
    17041 cgacattcat ccagcgccgc tggtagccag ccggaggcct ccgtttctac cataagcttg
    17101 gcggtgatcg gtaacagata ccagacatcc gccgctgcat attcacactg acgttcggtc
    17161 agcggtctgg ccagccagtc ggtgcgcgat tcactcttgt ccagcgtaac gccggaatac
    17221 tcttccacca tggaagcgaa accccatgac atcgggcgtc cgcagaaggc agcaaggatt
    17281 tgcgtgtcaa tcaggggttg tggtaattcg ccaaagacat tgaggaacac ttccagatct
    17341 tcactgcctg catggagaaa ttttgtgatg gacggatcgc gcaggatcgc tttcagcggt
    17401 gaccagtcgg tgatcccgag aggatcaatt agcgccagat gctcgccatc gaaaagttga
    17461 atcaacccca gctgcgggta ataagtgcgc gtacgaacaa attcagtatc cagggctatc
    17521 gccggaaagg cacggacggc ttcacacaaa gaagccagcg catcgtccgt ggtaatcatt
    17581 tggtaattca aattgttttc tctttagtgg gcgtcaaaaa aaacgccgga ttaaccggcg
    17641 tctgacgact gacttaacgc tcaggcttta ttgtccactt tgccgcgcgc ttcgtcacgt
    17701 aattctcgtc gcaaaatttt tccgacgtta gatttcggta actcatcacg aaactccacc
    17761 agcttcggta ctttgtagcc cgtgagctga cggcggcaaa aggtcaccag tgactcttcg
    17821 gtaagcgatg gatctttttt cactacgaag attttcaccg cttcaccact ggagccggaa
    17881 ggtacaccaa cagccgcgac ttcctgtacg ccaggatgct gcatgacgac atcttcaatc
    17941 tcgttgggat agacgttaaa accggaaacc aaaatcatgt cttttttacg atcgacaatg
    18001 cgcaggaatc cttcttcatc cattaccgcg atgtcgccag tgtgtaacca gccgtttttg
    18061 atgatttcat cggtagcatc cggacgctgc caatagccca gcatcacctg tggacctctg
    18121 acgcaaagtt cacctggctg acctggcgat acttcattat cgtcgtcatc caccagtttg
    18181 gcttccgtcg acggcaccgg caaaccaatg ctaccgctat gataatcaat atcatagggg
    18241 ttcacgctga ccagcggcgc acattcggtc aggccataac cttccagcag atactgtccg
    18301 gtcagtttca cccagcgttc tgccaccact tgctgaacag gcatcccacc gcctgcagaa
    18361 aggtgcagac tggagaaatc cagttgttga aactctttat tgttcagcaa cgcgttgaac
    18421 aaggtgttta cgccggtgat agcggtaaac ggatatttcg ccagctcttt gaccagcccc
    18481 ggaatatccc gcgggttagt gataagcagg ttctgcccac ccagttcgat aaacagcagg
    18541 cagttaatgg tcagtgcaaa aatgtgatac agcggcagcg ccgtcaccac cagctctttg
    18601 cccggatgca gcagaggccc atacgtagcg ttaacttgtt ccaggttcgc cagcatattg
    18661 cggtgagtca gcatcgcgcc tttcgccaca cctgtggtgc cgccagtgta ttgcaggaag
    18721 gctaaatctt ctggcaccag ttcaggtttg acatactgca tacggtagcc gttgtgcagg
    18781 gcgctacgaa atgaaatggc atctggcaga tgatatttcg gcaccagacg tttgatgtat
    18841 ttaacaacga agttaaccag tgtgcctttc gctgtggaaa gctgatcgcc catacgggtc
    18901 aggataacgt gctgaactgc ggttttatcg accacttttt ccagcgtgtg agcaaagttg
    18961 gacacaatga caatcgccga tgcgccgcta tcgttaagct gatgctcaag ctcacgcggg
    19021 gtatacaacg ggttaacgtt tacgacgatc atcccggcac gcaaaatgcc aaacagcgcc
    19081 accggatatt gcaataaatt aggcatcatc aacgcaacgc gatcgccttt cttcagcccc
    19141 aacccttgtt gcaaataagc ggcaaacgcg cgactgcgtt cttccagctt gcggaaggtc
    19201 atcacctccc ccatattcac aaacgcaggc tgatcggcgt agcgcgcgac cgactgctca
    19261 aacatatcta ccagagattg ataacggtca gggttgatct ccgtcggaac gtccgcggga
    19321 taacggttaa gccaaacctt cttcaattct tcacctctaa aatgcgtgtt cgtcgtcatc
    19381 gcaaccccaa atgatataca tgccgttaac ataatattaa ctcatcatac cagcttgata
    19441 attacccaac gaaaaggttg cgaagcgcgt cactatttat ttttatcttt accgtaagaa
    19501 tgcagaaaca gcggaccagc cgctgtttct ttttctttaa aaacaagcaa ttattcagtt
    19561 acaactgttt gtactctcgc ggggccggga ttataccagc cccatccgcc gtggccatag
    19621 ggccagccac gaccgccata aaaccaggga tcaatcggct gaggcggcat aatcacctgc
    19681 tgggttaaat gccaacgttt gtaacctgtt gcctgcatca ccataaattt atagggcgtg
    19741 ttgccgattt ttccgtcaac cgcaccagtg attggcccga ctaccgtaac cagttgtcca
    19801 cggaaatcca ccgggtccag aaaaccgttc acatcggcat aaatgcgacc gcgagaaggt
    19861 tctcccagcg tcggtctggc tccgctgtcc agcggtacgg tagcaatttc caggcgggtt
    19921 ttcccttgct ggttttgtac cgcaaccact ttgccaccaa agcgtgcctc ctgaccaacg
    19981 tacagctgcg gcgcactcat cacccgaact aaatcttgtt gcggcgtggg actgctgcct
    20041 ttaatggcgt ccggcacagt gacacaaccg ctcagcatta gcgcaaacgt acctgccagt
    20101 atgcctttga taacattctt ttgaaccgcc atgatgcgac tccttttttt ctcagggcat
    20161 actcttaaga ttcattcttt gcccggaagt ttcttccatg cgacgttgtt acgtaaataa
    20221 accggttcgg catgttccac cgccaccgtt ttaccctcgg caaacatctg acaagcaatc
    20281 ggcagcatat cttctgcagc gggcagtaaa acttctccgt cgcgcaaaac cagtccgctc
    20341 tctttaccga gatccggcca ggcttgccag cctgtaccta ccgtcaccca ttccccggaa
    20401 agctgttgca ttcgttcatg aacgagttcg ggtttgagta cggcttcggt ttcttcaccg
    20461 tgccagatac cgttttcatc acgctgatat tcggcccagt aaacttcgcc cattcgcgcg
    20521 tcaatggctg acagcacgcg tgttgcgccg tttttgcgcc acgccccttg cgccatcgtc
    20581 attagcgtgg agacgccaat catcggtaat tccgcgccaa gcgccagccc ttgcgcgatg
    20641 ccaataccaa tgcgcacgcc tgtaaagcta ccggggccgc gcccgtaagc cagagcgtta
    20701 atatcagtca gggaagttcc gctggtggtc aggatatcct gcaccatcgg taagattcgt
    20761 tgagtatgtt cacgagggca aagctcaaaa tgagcgttga cagtaccgtc gttccacagg
    20821 gcgacagagc aggcctctgt cgcggtatcg atagccagaa ttcgcatggg tcttcgtgct
    20881 tagatcaata aaaaggcgcg catcatacca tactccgtaa caaattacct ggaggatggt
    20941 atcgcaagga aacgaaccgc acgggcaatg tcacgggtgc gtggcgcggg cggcagactg
    21001 gcgagaaacg tcgcgccgta aggacgcatc accagccgat tgtcacaaat taccaaaacc
    21061 ccgcgatcgt cggcgtcgcg aatcagtcgc ccgactccct gcttgagagt aatcaccgca
    21121 tccggcagtt gtacttcatc gaacgggtcg ccaccgcgca aacggcaatc ttccatgcgc
    21181 gcttttaaca atggatcatc aggcgaggta aacggcaatt tgtcgataat caccaatgac
    21241 aatgtatcgc cacgcacgtc caccccctcc cagaaactac tggttgccac aagaagcgcg
    21301 ttaccggcac tgacaaattg ctgcaacagt tgccctttgc tggtttcccc ctgcaataat
    21361 acgggaagcg tcatggtagc gcggaactgc tcggccagat cgcgcatcat ggcgtgcgag
    21421 gtacaaagca taaaacagcg accgttgtta gcttcgatga tcgggcgcag catagccgcc
    21481 agttgccgcg cggaacccgg ttgattggtt tgcggcaggt tgcgcggcac acagagtaac
    21541 gcctggcggc tgtaatcaaa cgggcttggc aatagcaacg actccgcctg ttctatccca
    21601 agccgcgagg tgaaatgatg cagatcgtcg tttaccgaca gcgttgctga ggtgaagatc
    21661 cagctaccgg gcttttgcgc cattaactct ttgaatttat ccgccacgct gagcggcgtg
    21721 agagccagag taaaatggcg cgaagtgcat tcgtaccagt agctgtagcc cggctgattg
    21781 atctctttta gccgcttcag ccgtgtgcga tacaacgtgg cgcgctcaaa tgccgcatcc
    21841 agcaaggcgg aacgccccag tgacagtttc gccacgtcat aacaaagttc cagggtgtca
    21901 tcgagcagta aaaatgcccg ctgaatttgc ggattagcta acagctcacg caggttaccg
    21961 cgataacctg gttcaccgag ttgcagacga aaatcctgcg cactctgggc aagacgatca
    22021 gcgcactttt gtaactgctg ggtgtctttt aattcggtgc ggtaggcgat ggtgatgtct
    22081 tttgccaggt cgagcagttg tcgactggag agtgactgac caaaatactg gctggcaatg
    22141 tccggtagct ggtgggcttc gtcgaagatc atgacgtccg cttccgggat caactcgcca
    22201 aatccactct ctttaaccac catatccgcc agaaagagat gatggtttac caccaccaca
    22261 tcggcgtcca tcgctttttt acgtgctttg accacaaagc aatctttata catcgggcag
    22321 tcgctgccaa gacagttgtc gttggtgctg gtgaccagcg gccacgcctg cgaatcttcc
    22381 gccacgctga cgcaggtgct gatatcacca tcgactgttt gattagacca ggagcgcagc
    22441 aggatcacat cgcttaatat ttgtaccgga agatcgcccc ccgccagcgc ctgctgttcg
    22501 agacgttcga ggcagaggta gtttgagcgc cctttcagca acgccacgtt gcccgtgtat
    22561 ttcaacgcct ttgagaccgt cggcaaatcg cggctataga gctgatcctg caacgctttt
    22621 gagccggtcg agataatgac tttctttttc gcccgcagcg caggggccag gtaagcgtag
    22681 gttttgcccg taccggttcc tgcttccacc accagcggct ggcctttttc tatcgcctgg
    22741 gtgacggcta ccgccatctg tcgctgtggt tctcgcggct taaagcctgg tatcgctttc
    22801 gccagctgac cgtctggtgc aaaatcgtcc gtcacactac cccctgttga tttgaacagg
    22861 gattatgtca ggatgagggc gctttcgcca gttgaagtgg tgacggcgac ctcacattgt
    22921 ggcagtcttt gcagcacgaa aatggaagga taatgaggat aaaatgatga ctatcgttcg
    22981 tatcgatgct gaagcccgct ggtctgatgt agtaatccac aacaacacgc tctactacac
    23041 tggtgtaccg gaaaacctcg acgccgatgc ctttgagcaa accgccaaca ccctggcgca
    23101 gattgacgcc gtgctggaaa aacagggcag caataaatcg agcattctgg atgccaccat
    23161 tttcctggcc gataaaaacg acttcgcggc gatgaataaa gcgtgggatg cctgggttgt
    23221 cgcgggtcat gcgccggtgc gctgcacggt acaagcgggt ttgatgaacc cgaagtataa
    23281 agttgaaatt aagattgtgg ctgcggtgta agctttatcg aagcaaaata agtcagacga
    23341 taatttatcg ataatactgg tcggttatac ataaatc
//
LOCUS       DAZKIF010000078        23351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZKIF010000078 DAZKIF010000000
VERSION     DAZKIF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.078"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..516)
                     /locus_tag="ACHOTW_002936"
     CDS             complement(<1..516)
                     /locus_tag="ACHOTW_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9844999.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGH"
     gene            complement(573..1778)
                     /gene="rhmD"
                     /locus_tag="ACHOTW_002937"
     CDS             complement(573..1778)
                     /gene="rhmD"
                     /locus_tag="ACHOTW_002937"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFP9845000.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQLRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(1793..2575)
                     /locus_tag="ACHOTW_002938"
     CDS             complement(1793..2575)
                     /locus_tag="ACHOTW_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9845001.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(2795..3997)
                     /locus_tag="ACHOTW_002939"
     CDS             complement(2795..3997)
                     /locus_tag="ACHOTW_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP9845002.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(4097..4639)
                     /locus_tag="ACHOTW_002940"
     CDS             complement(4097..4639)
                     /locus_tag="ACHOTW_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP9845003.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            4918..5343
                     /gene="nudI"
                     /locus_tag="ACHOTW_002941"
     CDS             4918..5343
                     /gene="nudI"
                     /locus_tag="ACHOTW_002941"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP9845004.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            complement(5382..5984)
                     /gene="ais"
                     /locus_tag="ACHOTW_002942"
     CDS             complement(5382..5984)
                     /gene="ais"
                     /locus_tag="ACHOTW_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP9845005.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            6292..7431
                     /gene="arnB"
                     /locus_tag="ACHOTW_002943"
     CDS             6292..7431
                     /gene="arnB"
                     /locus_tag="ACHOTW_002943"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP9845006.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            7435..8403
                     /gene="arnC"
                     /locus_tag="ACHOTW_002944"
     CDS             7435..8403
                     /gene="arnC"
                     /locus_tag="ACHOTW_002944"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP9845007.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            8403..10385
                     /gene="arnA"
                     /locus_tag="ACHOTW_002945"
     CDS             8403..10385
                     /gene="arnA"
                     /locus_tag="ACHOTW_002945"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP9845008.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            10382..11272
                     /gene="arnD"
                     /locus_tag="ACHOTW_002946"
     CDS             10382..11272
                     /gene="arnD"
                     /locus_tag="ACHOTW_002946"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP9845009.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            11272..12924
                     /gene="arnT"
                     /locus_tag="ACHOTW_002947"
     CDS             11272..12924
                     /gene="arnT"
                     /locus_tag="ACHOTW_002947"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP9845010.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            12921..13256
                     /gene="arnE"
                     /locus_tag="ACHOTW_002948"
     CDS             12921..13256
                     /gene="arnE"
                     /locus_tag="ACHOTW_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP9845011.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            13256..13642
                     /gene="arnF"
                     /locus_tag="ACHOTW_002949"
     CDS             13256..13642
                     /gene="arnF"
                     /locus_tag="ACHOTW_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP9845012.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(13636..13902)
                     /gene="pmrD"
                     /locus_tag="ACHOTW_002950"
     CDS             complement(13636..13902)
                     /gene="pmrD"
                     /locus_tag="ACHOTW_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP9845013.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(14012..15367)
                     /gene="menE"
                     /locus_tag="ACHOTW_002951"
     CDS             complement(14012..15367)
                     /gene="menE"
                     /locus_tag="ACHOTW_002951"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP9845014.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(15364..16326)
                     /gene="menC"
                     /locus_tag="ACHOTW_002952"
     CDS             complement(15364..16326)
                     /gene="menC"
                     /locus_tag="ACHOTW_002952"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP9845015.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(16326..17183)
                     /gene="menB"
                     /locus_tag="ACHOTW_002953"
     CDS             complement(16326..17183)
                     /gene="menB"
                     /locus_tag="ACHOTW_002953"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP9845016.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(17198..17956)
                     /gene="menH"
                     /locus_tag="ACHOTW_002954"
     CDS             complement(17198..17956)
                     /gene="menH"
                     /locus_tag="ACHOTW_002954"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP9845017.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(17953..19623)
                     /gene="menD"
                     /locus_tag="ACHOTW_002955"
     CDS             complement(17953..19623)
                     /gene="menD"
                     /locus_tag="ACHOTW_002955"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP9845018.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(19712..21007)
                     /gene="menF"
                     /locus_tag="ACHOTW_002956"
     CDS             complement(19712..21007)
                     /gene="menF"
                     /locus_tag="ACHOTW_002956"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP9845019.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(21086..21391)
                     /gene="elaB"
                     /locus_tag="ACHOTW_002957"
     CDS             complement(21086..21391)
                     /gene="elaB"
                     /locus_tag="ACHOTW_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP9845020.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(21446..21907)
                     /locus_tag="ACHOTW_002958"
     CDS             complement(21446..21907)
                     /locus_tag="ACHOTW_002958"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9845021.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            21972..22889
                     /gene="rbn"
                     /locus_tag="ACHOTW_002959"
     CDS             21972..22889
                     /gene="rbn"
                     /locus_tag="ACHOTW_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP9845022.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            23077..>23351
                     /locus_tag="ACHOTW_002960"
     CDS             23077..>23351
                     /locus_tag="ACHOTW_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFP9845023.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIKLIKNACL"
BASE COUNT         5657 a         6230 c         5830 g         5634 t
ORIGIN      
        1 atgccccata aatccatgca tctccagcag cgcgccggaa agcggtgatc ccagtgttaa
       61 cgccagcggt gcgcccatat agaacagccc cataatgctg gcgcgattac gctgcggaaa
      121 ccattgcgag gtgagataaa tcataccagg gaaaaatccg gcctccgcag caccaagcag
      181 agtgcgaact atcagaaatt tcgcttcagt atcggcccat gccatggctg ccgaaagaaa
      241 tccccacagc agtgttgtcg taccaatcca ggttctggcc cccagtttgc gcatcaaaag
      301 attcgccgga acacccagaa acgcatatac cacaaagaaa atgcctgctc ccagcgcata
      361 agcttcatta ctcaacccgg tatcaatctg gtaggtctgt ttggcaaaac cgatattcga
      421 acggtcgaga aacgccagca catacagcgc caacataaac ggaattaaac gcacacggtt
      481 tttcttcacc acggcgtcaa gcaaagcggt gctcataaaa agctccttag aatatgtggg
      541 cgttaccgtt gtcacagcaa cgcccggagt gattagtggc tgtaggggcg tttcaaattg
      601 cagtcacgat tgagttcgac gccaaaaccg ggtttatcaa gcactgattt atgaatacga
      661 ccattcaccg gaaccggctc attgagcaga atcgggtcaa actgcggacg catcgttgaa
      721 caatccggac tagtcatcag gaattcgctg aatggcgtat tagtgaaggt gatcaccgca
      781 tggtgggagt aaacagacga accgtgcggc accaccagtt gccccctgga tttggcgatt
      841 gcggcaattt ccaccagcgt ggttaagcca ccgcaccagc caacatccgg ctgcataatg
      901 tcgataccgg tttctgaaag cgtacgaaaa gattgcagtg tgccgtggtg ctcaccgctg
      961 gtgaccatca ttccgactgg cgcgttgcgt ttcagttcgc gataaccttc atactgctgt
     1021 ggcggcaggc actcttcgat ccatttcagg ttatagggcg cgcaagcgtg ggccagtttg
     1081 gtcgcatagt tcacgtcctg actcatccag cagtcgagca ttaaccagaa atcctcaccg
     1141 catttttcac gcatatccgc gaccatagcg gcatctttgc ggatccccgc atcgccatca
     1201 tgtggccccc agtgcgtcgg cattttgcca ccgataaagc ccatctcttt tgccagatcc
     1261 ggacgcgcac ctgtggcgta gaactgaatc tcatcacgaa cagcgccgcc taaaagttta
     1321 taaaccggaa gcccgaccac tttgccgaac agatcccaca gagccagatc gacacaggaa
     1381 atcgtgttca tcaccaggcc accagagccg gagtaataca gggtggcatt gagcatttga
     1441 tcatggatca gtttgatatc actgacacat ttaccctcaa tgaaacggtt aaggtgtttt
     1501 tcgacgataa aacagcccat ttcaccggct gtcgaaacag cgaatccggt ctggccgttt
     1561 tctgcttcga cttcaacaac caacgtgcca agaacgttaa tgccaaatga ctggcgtaac
     1621 tgctcgtaat cgcggtattt actcatcggg gtggcaatat gatcgtctat ccagtgattc
     1681 gccccctggt cgtgataatc accgccgcca gcgccttttt ctgctgtcgc accgccagta
     1741 aaccaggcgc gaacctgttt aatttttggt agggtcatga tgttctccat tgttatgagg
     1801 cttgtaagtc aaagggactt ttccatccca acagacgtga aatatccctg gcgcaggcaa
     1861 tggccttgcc cgccagataa tcacggtatt cttcattgat ttgtaagcgg gtaccgacca
     1921 ccgagatcgc agcggtaagc tcgttattgg cgttaaacac cggcgcagcg acacaacgga
     1981 catcggcgta atcttcgccg ttgtcatagc tccagccctg acggcgaata cgcgccagtt
     2041 cttcgtgaag ttgctgtgga tgagtaatcg ttgtgggtgt cgcctgctcc cagaccagcc
     2101 cttcgataat actttgctgt acagttgcag gttgccaggc aagcaggcat ttacctattc
     2161 cggagcgata aagcgaaagg ctttttcctt catgtgaacg cacgctgata gtggctgatg
     2221 actccacttt caaaatgtaa taagcgctgc cgttatcaat aatccccagg tgacataaca
     2281 gcccggtggt atccatcagt tgcgtcaaac gcggtcgagc cagttcccgg agatccatct
     2341 tgcttaacgc atgaccggaa agctccacca gcttggtcca caaacaaaaa ttttcctggt
     2401 gatcaaggct aagaaaacgc tgacgcctga gttcattaag cagcaaatag gccgtgcttt
     2461 tagggatccc caatgtgtca atgatcgtcg cagcattaca ggggccaatg cgggcaatca
     2521 aattgagaat atcaatagcc cgtgtcaggg cgggaacttt gcttgattcc aacatactgg
     2581 actccagtct tagatactgg aatcagtctc gttgctcagg gggagtaaaa ttgtgaagcg
     2641 catcaaatcc cgctcctcgt ccaacggact ggagaaatta cgcacaaatt tgtgaatcag
     2701 cgcacggatt ttttcttatt gagtacaaca ggttggacaa gttttgccgg agggggaatg
     2761 tgccggtgca gtaaaccggc acagagtggc ggaatcagac ggataaggtc atggactcaa
     2821 caacctcaat ccagccatgc tcactggcaa tgtcctggcc gtttaaccaa cggcgtagca
     2881 tattcagtgc catcattgcg cacacttcct gacggatagc caggctatag cgtgtagtgc
     2941 tgaaacgcac acgcagagct aaagtgccat ctggcgtggc tagcgcaaag ttgagatgct
     3001 cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggct cgccgttctg
     3061 taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaaccact tcacacgcca
     3121 gcagtggagc acctgcgcga gaaagttgca aagccaataa accaccggtg aattgctcgc
     3181 tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg ggcagtcctt
     3241 cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc cacagttttt
     3301 ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc ggcattgagg
     3361 agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt tgtgccagat
     3421 cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt ggtaaagaaa
     3481 aacgctcgcg cagacgcggc aggatttcgt gctcgaccat caccttaaat tctgacggta
     3541 cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca caggcggtgc
     3601 ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta cggttgctcg
     3661 gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc caggcttcat
     3721 gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa tcatcgctgg
     3781 tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt tcgcgaagaa
     3841 tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat ggcaacccct
     3901 gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc ccgtgtaaca
     3961 cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc ttttgttatc
     4021 gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatggc gcgacagaat
     4081 gccgcgccgg aagggatcag aaactggcgt taaccccaac atacgggccg tcagccacgg
     4141 cgttgtcgcg gttaccgtct ttacccgaca gattcaggta gcgataaccc gcctcaatac
     4201 tgactggacg cataatggtg taacgcgcgc cagcattcgc ttcctcataa cttttaatac
     4261 cgctggagag tgaatccgga gagtagtaat actcgccaaa caaacggaag ctgttgccaa
     4321 ttttccactg caaaccacct cctaccgctg cggcatagcc ttcatcgccg taattcgggt
     4381 tggtgtaaac gccttttccg ccaacggtcg ccattaacgg cccgagaggc agattcaacc
     4441 ccagccccac gcccgcgacg tcaccgtcgt cgtcgttatg tgtccagtta ccgcttaaag
     4501 ccaggcccgt cgattcagtc ccaaaaccga caccaatatt ggtgtattct ttacccgcct
     4561 ggccgctgat gctcattgca ttgaccgatg cagaaaccaa cagcattccg gccagacctg
     4621 ctagcgcaat ttttttcatt atcccctttc ccacaagcaa ttaatcaaaa ttcccaaagc
     4681 tgcgggattg tactgcctgg cgcagaggat tcaatgagcg aaaaaaggga tctagtcact
     4741 tattgtaact aaatacttct taaataatta aacgccgaaa aaataaacac agtttaccga
     4801 tacacacaaa aataataata agtgcagtta tcatcgcaaa agagattact gtcactttca
     4861 catatttctg agttaccgta ttctcatggt ttctttttat ttataaggaa tacccctgtg
     4921 cgacaacgga ctattgtatg ccctttgatt caaaatgatg gtgcttattt gctgtgtaaa
     4981 atggccgacg atcgcggcgt tttccccggt caatgggcgc tttcgggtgg cggcgtggag
     5041 cccggcgaac gaattgaaga ggctctacgc cgcgaaattc gcgaagaact gggagaacag
     5101 ctgcttttga cagaaatcac gccctggacc ttcagcgatg atattcgcac caagacgtat
     5161 gcagatggtc gcaaggaaga aatttatatg atttacctga tttttgattg cgtttctgcc
     5221 aaccgagaag tgaaaataaa cgaagagttt caggactacg cgtgggtaaa acctgaagat
     5281 ctggcgcact atgatttgaa tgtcgccacc cgaaaaacgt tacgtttgaa aggtcttctg
     5341 taacaatgat gacagccatt actggctgtc agattgaaaa tttagtgatt aacgaattcc
     5401 ccatccagat aaactttgcc tttttccaca tgcatgacta aaccatccag ataatcaggt
     5461 ttaaatgtcg catcacgctt atctttagca atatatgtca ggcaatgatt atgggtgaaa
     5521 ataacgatat ttttatcagg cgctttgctt tgtaagtcct taatcgcact ataaatctca
     5581 ttaccgcact gaagaagtcg tttatctacc gtcaattttt tacccgctga aaaccaggtc
     5641 gcggactgaa tggtccggac ggtattactg gaataaagat cgaaatcagg gatatcagca
     5701 ctaaaagcgt tgcccagttc acgggcatcc tgggtacctt taaccgtaat acctgtttta
     5761 tctgacaagc attgattggt tgaacgatcg caacgttcag catggcgaaa caaaacgaca
     5821 accgggtgct gctgtgccag tctgcccagc gttttattgt cgatacgtgg caaaccattg
     5881 ctactccagg cggcatgagt acccagtccg gcaattgcag cgagcgccag taaaatgatg
     5941 aaatattttt ttgacttcaa tgatgagcgg caaaaagcta acacgtaaac tccacctata
     6001 gacaagcgca accagacaat taccgtgaaa ttgagcgaca tttctggcga taattagcag
     6061 ttggtgtaat attaaaaatc ctacgatgct ggcaaaatgc ctcaaaattt tgccaaatgc
     6121 aaagcctaaa taagaaaaaa tataaaaatt tcaatattta cgtctaatat tagtttctta
     6181 aggttaagtt aatattctat ccttaaaatt tcgctctaaa tggcaaaata tacacaacac
     6241 tctttatagc aaatataagt ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa
     6301 tttttgcctt tttcgagacc agcaatgggc gtggaggaac tcgctgcagt taaagaggtt
     6361 ctcgaatccg gttggatcac aaccggtccg aagaatcacg cgcttgaaca agccttttgc
     6421 cagttgacgg gaaatcagca tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc
     6481 acgctaatgg cgttggaaat tggcaagggc gatgaagtga ttacgccttc cctgacctgg
     6541 gtttcaaccc tcaatatgat ttccttgcag ggtgcaacgc cggtaatggt ggatgtcgat
     6601 cgcgatacgc tgatggtcac gcctgaagcg atcgaatcag ccattacgcc acgcactaaa
     6661 gccatcattc cggtgcatta tgccggtgcg ccagcagata ttaacgccat tcgcgccatt
     6721 ggcgaacgtt acggcatcgc agttatcgaa gatgctgccc atgccgtagg tacgtattac
     6781 aaagggcgac atattggcgc aaaaggtacc gctatttttt catttcatgc cattaaaaat
     6841 attacctgtg cggaaggagg cctgattgtt actgataatg aaaatattgc ccgccagcta
     6901 cggatgctga aatttcacgg cctgggtgtc gatgcctatg acagacaaac ctggggccgt
     6961 gcaccgcagg ctgaagtctt aacgccgggc tataagtaca atctgaccga tattaacgcc
     7021 gcgattgccc tgacacagtt agccaaatta gagcacctca acacccgtcg gcgcgaaatt
     7081 gcccagcaat atcagcaagc actggcagct ctcccctttc agccattaag ccttcccgcc
     7141 tggccgcacg ttcacgcctg gcatctgttt attattcgtg tcgatgaaca acgttgtggt
     7201 atcagtcgcg atgcgttgat ggaagcgtta aaagaaagag gtattggtac cgggttacat
     7261 ttccgcgccg ctcacacaca aaaatattat cgcgagcgtt ttccctcgct gtcgttaccg
     7321 aataccgaat ggaatagcga acgcatctgt tctttgccgc tgttcccgga tatgactacc
     7381 gccgatgccg accgtgtcat cacagccctt cagcaactcg caggacaata agccatgttt
     7441 gaaatccacc ctgttaagaa agtctcggtg gttattcccg tttataacga gcaggaaagc
     7501 ttaccggaat taatcaggcg caccaccaca gcctgtgaat cgttggggaa agagtatgag
     7561 atcctgctga ttgatgacgg cagtagcgat aattccgcgc atatactggt cgaagcctca
     7621 caagcggaga acagccatat tgtatctatt ttgcttaacc gcaattacgg gcaacattca
     7681 gcgattatgg cgggattcag tcacgttact ggcgacttaa ttattaccct tgatgccgat
     7741 ctccagaatc cgccagaaga aatcccccgc ctggtggcaa aagccgatga aggttacgac
     7801 gtggtaggga ctgtacgcca gaaccgccag gacagctggt ttcgtaaaac agcttcgaag
     7861 atgattaacc ggcttattca gcgcaccacc ggcaaagcga tgggtgacta cggttgtatg
     7921 ctgcgcgcct atcgccgtca tattgtcgat gcgatgttgc actgccatga acgcagcacc
     7981 tttatcccga ttctggcgaa tatcttcgcc cgccgtgcca ttgaaattcc agtgcatcat
     8041 gccgagcgtg agtatggtga atccaaatac agctttatgc gcctgattaa tttgatgtac
     8101 gacctggtga cctgccttac cacaacgccg ctacgtatgc tgagtctgct cggcagcatt
     8161 attgcgattg gaggttttag cattgcggtg ctgctggtga ttttacgcct gaccttcgga
     8221 ccacaatggg cggcagaagg cgtctttatg ctatttgccg tgctgtttac ttttattggc
     8281 gctcagttta tcggcatggg attactcggt gaatatatcg gcaggatcta caccgatgtc
     8341 cgcgcccgcc cccgctattt tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa
     8401 taatgaaaac cgtcgttttt gcctaccacg atatgggatg cctcggtatt gaagccctgc
     8461 tggctgccgg ttacgaaatt agcgccattt ttacccatac cgataatccc ggtgaaaaag
     8521 ccttttatgg ttcggtggct cgtctggcgg cggaaagagg cattccggtt tatgcgccgg
     8581 ataacgttaa tcatccgctg tgggtggaac gcattgccca actctcacca gaggtgattt
     8641 tctcttttta ttatcgccat cttatttgcg acgagatttt gcagctcgct cccgcaggtg
     8701 catttaatct gcacggttcg ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg
     8761 tgctggtgaa cggtgaaaca gaaactggcg ttacattgca ccgaatggta aaacgtgccg
     8821 atgccggggc cattgtggcg caactgcgcg ttgccattgc gccagacgat atcgctatta
     8881 cgctgcatca taaattgtgc catgccgcgc gccagctact ggaacagaca ttacccgcca
     8941 ttaaacacgg taatattctg gaaatcgccc agcgcgaaaa cgaagccacc tgttttggtc
     9001 gcagaacgcc ggatgacagt ttcctcgaat ggcataagcc ggcatccgta ctgcacaaca
     9061 tggtacgtgc cgttgccgat ccgtggccgg gtgccttcag ctatgttggc aatcagaaat
     9121 tcaccgtctg gtcgtcgcgt gttcatcctc atgccagcaa agcacagccg gggagcgtga
     9181 tttctgttgc gccactgctg attgcctgtg gcgatggcgc gctggaaatc gtcaccggac
     9241 aagcgggcga cggcattact atgcagggct cgcaattagc gcagacgctg ggcctggtgc
     9301 aaggttcacg cttgaatagc cagcctgcct gcaccgcccg acgccgtacc cgggtactca
     9361 tcctcggggt gaatggcttt attggcaacc atctgacaga acgcctgctg cgcgaagatc
     9421 attatgaagt ttacggtctg gatattggca gcgatgcgat aagccgtttt ctgaatcatc
     9481 cgcattttca ctttgtcgaa ggcgatatca gtattcattc cgaatggatt gagtatcacg
     9541 tcaaaaaatg tgatgtcgtc ttgccgctgg tggcgatagc cacgccgatt gaatataccc
     9601 gcaacccgct gcgcgtattt gaactcgatt ttgaagagaa tctgcgcatt atccgctact
     9661 gcgtgaagta ccgtaagcga atcatcttcc cgtcaacttc agaagtttat gggatgtgta
     9721 gcgataaata cttcgatgag gaccattcta atttaatcgt cggcccggtg aataaaccac
     9781 gctggattta ttcggtatca aaacaattac ttgatcgggt gatctgggcc tatggcgaaa
     9841 aagagggttt acagttcacc ctcttccgcc cgtttaactg gatgggacca cgactggata
     9901 accttaatgc ggcacgaatc ggcagctccc gcgcgattac gcaactcatt ctcaatctgg
     9961 tagaaggttc accgattaag ctgattgatg gcggaaaaca aaaacgctgc tttactgata
    10021 ttcgcgatgg tatcgaggcg ttataccgca ttatcgaaaa tgcgggaaat cgctgcgacg
    10081 gcgaaattat caacattggc aatcctgaga acgaagcgag cattgaggaa ctgggcgaga
    10141 tgctgctggc gagcttcgaa aaacatccgc tgcgccatca tttcccaccg tttgcgggct
    10201 ttcgcgttgt cgaaagtagc agctactacg gcaaaggata tcaggacgta gagcatcgta
    10261 aaccgagcat ccgcaatgcc caccactgcc tggactggga gccgaaaatt gatatgcagg
    10321 aaaccatcga cgaaacgctg gatttcttcc tgcgcaccgt tgatcttacg gataaaccat
    10381 catgaccaaa gtaggcttac gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt
    10441 gccgcgtctg ctggaaatct tgagtaagca taatattcag gccagcattt ttttcagcgt
    10501 cggcccggac aatatgggcc gccatctctg gcgactggtg aagccacagt ttttgtggaa
    10561 gatgctgcgc tcaaacgcgg catcgcttta tggctgggat attttactgg caggtacggc
    10621 ctggccaggt aaagagattg gtcatgccaa tgccgatatc attcgtgaag cggctaaata
    10681 tcacgaagtc ggcctgcacg cctgggatca ccatgcctgg caagcccgta gcggtaactg
    10741 ggatcggcaa acaatgatcg acgatattgc acgcggcctt cgcactctgg aagagattat
    10801 cggtcaaccg gtaacctgtt ctgccgctgc gggctggcgt gccgaccagc aggtgatcga
    10861 agcaaaagaa gcgttccatt tgcgctacaa cagcgattgt cgtggggcca tgccgttccg
    10921 tccactgctc gaatcaggaa accctggcac tgcgcaaatt ccggtgacct tacccacctg
    10981 ggatgaagtg attggtcggg atgtgaaagc agaagatttt aacggttggt tactcaaccg
    11041 catcctgcga gataaaggca cgccggttta taccattcat gcagaagttg aaggctgcgc
    11101 ttatcagcat aattttgtgg atctcctcaa acgcgcagct caggaaggcg tgacattttg
    11161 ccctttaagc gaactgttat cagagacgtt gccgctcgga caagttgttc gcggaaatat
    11221 tgccggacgt gaaggctggc tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg
    11281 gtacgttacc ttatcggcct cttcgcgttt attgcctgct attacctgtt accgatcagc
    11341 acgcgtctgc tatggcaacc cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg
    11401 gcatccggcg actggattgt gccccatctg ttagggctac gttattttga aaaacccatt
    11461 gccggatact ggattaacag cattgggcaa tggctatttg gcgcgaataa ctttggtgtg
    11521 cgggcaggcg ttatctttgc gaccctgtta actgccgcgc tggtgacctg gtttactctg
    11581 cgcttatggc gcgataaacg tctggctcta ctcgccacag taatttatct ctcattgttt
    11641 attgtctatg ccatcggcac ttatgccgtg ctcgatccgt ttattgcctt ctggctggtg
    11701 gcgggaatgt gcagcttctg gctggcaatg caggcacaga cgtggaaagg caaaagcgca
    11761 ggatttttac tgctgggaat cacctgcggc atgggggtga tgaccaaagg ttttctcgcc
    11821 cttgccgtgc cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat
    11881 ctctttattt acggctggct ggcggtcatc agttgcgtac tgacggttct cccctgggga
    11941 ctgacgatag cgcagcggga gcctgacttc tggcactatt ttttctgggt tgagcatatt
    12001 caacgctttg cactggatga tgcccaacat agagctccgt tctggtacta cgtgccggtc
    12061 atcattgccg gtagtctgcc gtggctggga ttactccccg gtgcactata cacaggctgg
    12121 aaaaaccgca agcattccgc aaccgtctat ttgttgagct ggacgataat gccgctgctg
    12181 tttttctccg ttgctaaggg taaattgccc acctatattc tttcctgctt tgcacctttg
    12241 gcaatgctga tggcgcatta cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg
    12301 attaatggct ggattaacat cgcttttggc gtcactggca ttattgccac atttgtggtc
    12361 tccccctggg gaccaatgaa cacgccggtg tggcaaacct tcgagagcta taaagtcttt
    12421 tgtgcctggt cgattttttc gctatgggca tttttcggct ggtacacctt aacaaacgtc
    12481 gaaaagacct ggtcttttgc cgcgctttgc ccgctggggc tggcgttgct ggtaggattt
    12541 tcaattcctg acagagtcat ggaaggaaaa catccgcaat tttttgtcga gatgacacaa
    12601 gaatcacttc agccaagccg ctatattctt actgatagcg tcggtgttgc cgcaggtctg
    12661 gcatggagtc tgcaacgcga tgacatcatc atgtatcgcc agacaggtga gttgaaatac
    12721 ggccttaatt atccggatgc gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg
    12781 cttaatcaac atcgtcagga ggggattatt acactcgtgc tttcggttga ccgcgatgaa
    12841 gatatcaaca gtctcgccat tccgcccgca gatgccatcg atcgtcagga gcgtctggtg
    12901 ctgattcagt atcgtcccaa atgatctggc taacattagt ctttgccagc ttgcttagcg
    12961 ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt ggcgataagc aaacggcgca
    13021 aacatatcgt gctgtggctg ggactggcgc tggcttgtct tggtcttgcc atggtgctct
    13081 ggctgctggt cttgcagaac gtaccggtag gtattgccta cccgatgtta agtctgaatt
    13141 ttgtctgggt gacgctggct gcagtaaaac tgtggcacga accggtatcg ccgcgtcact
    13201 ggtgtggggt ggcgttcatt attggtggca ttgtgatcct cgggagtacg gtgtaatggg
    13261 cctgatatgg ggattattca gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt
    13321 tgcggcgagt catctgccgc cgatgacgca cttatgggat tttatcgcgg cgctgttagc
    13381 ttttgggctg gatgcccgga tactgttact cgggctgctg ggttatctgc tttccgtttt
    13441 ttgttggtat aaaacactac ataaacttgc gctaagcaaa gcctatgcct tgctcagtat
    13501 gagttatgtg ctggtatgga ttgcttcaat ggtacttccc ggctgggaag ggactttttc
    13561 gctgaaagca ctactgggag tagcctgtat tatgagcggg ttgatgctga ttttcctgcc
    13621 cacgacaaaa caacgttact gagttttccc tgccacttta cactgccgtt cccactcatc
    13681 ggggctataa cagcttgcag agaggacttt caccgtatgc agcttttcac gattaataca
    13741 ataaagcgca ttctgcaaag gcgagagtaa atcccccact ttcacggcga agtcactctt
    13801 cacttcggcg atcattttta tcgcgccgcc agcatcgcag agcataagta catgtctgtt
    13861 atcttgttta ttgcagcacg attttttgac cagccattcc attgtattat cctgtttgct
    13921 aagagttttc atatcaattt acagattaac agataaactc tttatcaacg gcaatggaat
    13981 ggcagaactg agaagtgaaa cggagggtta attattgctg acgttgcacc cactctttta
    14041 gcgcctgacg tgaaatttta ataccgccgt ttttcagctc cggcggcaaa gtcagccaac
    14101 gtaccggttg ctgaaaacgg gccagcttat ctttcaccca ttcactaaga tcaacgctct
    14161 cgtggtcata ctccatcacc gccaccggtc gatgaccaaa ctccttgtcg gcaacgggga
    14221 cgataaacac ctgcaaaacc gcaggatgtg cagcaattac gcgctcgact tcttccgtct
    14281 gaataccctc tccgccactg aagaatagat tgtctaaacg tccgacaatg gtcagcttgc
    14341 cattatgcat ctcaccgcga tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa
    14401 ccagttgccc gttacgccag taaccttctg ccatactggc agcccgcagc cacacttcat
    14461 tattaacgat tttcacttcc cgacccggca gcggcgaacc aacgtctgcc aggccgtcgg
    14521 cttctttcgc acacaccgtg gaggcaaact cggtcagacc atagccgcaa aagcaacgaa
    14581 tcccctgctc gcgcgcctgt tccgtcaact cgaccgggat agccgcgccg ccaagtaaca
    14641 ccgctttcag ggaaacggaa ctgcggttaa ccagcaaacg ccagagttgt gttggcacca
    14701 gtgaagcgtg agtacagcct gccagcattt gctccaatgg ctgtttatca cgcaccgtca
    14761 tccgcgcacc agcgtataac cagcgccaca taattccctg accggagacg tgaaacagcg
    14821 gtaaagagag caaccagtca tcgtgatcgc caaacggaat cagcgataac accccttcgg
    14881 cactggcaag atgggcctga taagtatgga cagcggcttt cggcaacccg gtagaacccg
    14941 aggtcaacgt cattgagcac agacgcgtcg gctgccacgt agcgacatgt gcgccttcaa
    15001 cccgctgaat gtgcagcgac gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg
    15061 caaattgcag cgtcagattg ggcagcaatt cttcaagcaa cggttgcggc agctgagggt
    15121 tcacgggcaa cacccgcgcc ccgcattgca gtaacgccag ccaagcgagc agcgtttgcg
    15181 gcgtattcca cgcccgcaac atcacgccgc tgtcctcaac cacaccctgc accgcaaatc
    15241 cggaggctaa ttcatcgacg cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa
    15301 gacgtaaggc gatggcttct ccccgcactt gccgccagtg acgccacggc cagtcagaga
    15361 agatcataac aaccgctcca gtgcatcaac ttccacgaca ggcagcgtgc tacccggcca
    15421 gcgacgtacc tgctgcgcct gcattagatc cagcgtgtcc agccctggaa tggtgtccgg
    15481 cgttaaccag gcagcaatcc gcgccagttg cgttaagcct aagctcgatt caatggaaga
    15541 actgatcact gccgtcagcc ccagcgcgtg cgccgcctgt acctgctcgc gaactttctc
    15601 cagactgccc gtgagcgtgg gtttgataac taccgcgcgc acgccctctt cagccacaaa
    15661 ggcaaaatcc ggctcgcgca agctttcatc ccaggcaatg gcaatgccgg tttcacgggc
    15721 aaacgctcgc gaatcatcgc gggttttgca cggctcttcg agaaacgcga tgcggtggcg
    15781 ataatccggg ttaacgtatt tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt
    15841 tgcgtcaaga cgcaaatgca gatccggaat tgcctccagc aacagattca ccaccatgcc
    15901 gtcgcgcacc gcttcgtaca atcccacttt gaccttcgcc actttctcgc ctggcatatc
    15961 tgcaagtttg aggatcagat cgtccggatc gccattacac agtggtgccg tacggtaatt
    16021 ggccgcctgc gggagcgtat ctgccagctc cgccaatgca caacttacgc caaaggcaac
    16081 ggaaggcatc tgcggtaatt cgcaatcgcc tgccagccag ttatttaccc aggcaagcag
    16141 cacactttgc gcctcttccc aggtttcctg actgaagccc ggcagtgggg agatctcccc
    16201 ccacccttcg cgctcgccct cacgcaggca aacgtacagc ccgtcgcggg tttttaaccg
    16261 cctgtcgcgc agaaccaccc ccgcgtccat ggggatctgc cagcggtata cctgcgcgct
    16321 acgcattacg gattccgttt gaatttgctg aagtcaggct gacgtttctg gttgaaggcg
    16381 ttgcgacctt cctgaccttc ttccgtcatg tagaacagca tggtggcgtt gcccgccagc
    16441 tcctgcagac ctgcctgacc gtcacagtcg gcgttcagtg cagctttcag gcagcgcagc
    16501 gccatcggac tgttttgcag catttcacgg caccagcgaa cggtttcttt ttccagatcc
    16561 gccagcggta ccacggtgtt gacaaggccc atatccagcg cctgttttgc gtcgtactga
    16621 cggcacagga accagatttc acgcgctttt ttctgcccga cgatacgagc catgtaggaa
    16681 gcgccccagc cgccgtcgaa ggaaccgact ttcgggccag tctgaccgaa gatggcatta
    16741 tctgccgcga tagtcaggtc gcacatcatg tgcagaacgt gaccaccgcc gatggaatag
    16801 ccagccacca tcgcgacaac cggtttcgga caggtacgga tctgacgctg gaagtccagc
    16861 acgttcaggt gatgtacgcc ggaatcatct ttatagccgc cgtaatcacc acgcactttc
    16921 tggtcaccac cggagcagaa cgctttatcg cctgcgccag tcagaatgat cacgccgacg
    16981 ttatcgtcat aacgcgcatc tgccagtgcc tggatcatct cttttaccgt cagaggacgg
    17041 aaggcattgc gcacctgcgg acgattaatg gtgatttttg cgataccgtc ggtggatttt
    17101 tcataacgaa tgtcctcaaa gccttcggag cagtcgtgcc attcaaccgg tgcgtaaagc
    17161 attgcttcat caggataaat catattgtgt cctttagtca gaaacgcaag atctgcgcca
    17221 gacttgcgat tacgccagcg ggattttccc gatgcgcgtt atgtccggcg cgaggaatga
    17281 catggcagtc ggcagccagt tccgccgcca gggcgcggaa tttgctgtca cgttcaccac
    17341 ataaataata aaacgcaaat gtgcgggcgc taaggttagc acgtaaatca ggctggacgg
    17401 cgagagaagt cgcctccagc atggcggcaa gggttgcgcc attattgttg ctgcgcagca
    17461 ccaccagctc ccggcgttga tcgtcattga gcgaggcaaa aacaggctgt tgataccagt
    17521 cggcaaatac cgcggttaat ggttcggaac gaaaacgctg cgcccattgg cgatcggaac
    17581 gctgacgttc cgcgcgttgt tcagcatttt gcagccccgg atgaccgcct tcgacaacaa
    17641 ccccacaaag ccccgccagc ccctggcaag cggccatcat cgccacccgt ccgccaagcg
    17701 agtaccccac cagccagaag ctaaggatgt tgtaactaac cagggtttta cgcagtaagt
    17761 cggtgacatc atcaaatcca tcgacgctaa tcgccgccga accaccgtga cctgggagat
    17821 caacatacaa ccgtgagtag tcggcaaacg cctcgccgac ttcctgccat tcgaggcaat
    17881 cgccggaaaa accgtgcaaa aacaccagcc agggtaaacc tggtttgccg tgttttgcct
    17941 gcgcgtgcag gatcataaat ggcttacctg cgccagcagc tgctggagcg tttgcgcgcc
    18001 gtcggtgtcg ttaaccacca tttcaattac cgtggtggtc ggagtacgcc aggcgtcggc
    18061 aagtaccgtt tcaagttcct gccagttttg cggacgatga tatttcagct caaacatcgc
    18121 ggcagcgtgc tcaaaatgga cgttttgcgg catcagatag aaacgctcgc gctcgctttt
    18181 cggcgttggc agcagcgaga aaatttgccc gccgttgttg ttcaccacaa ttaataccag
    18241 cggcgcggaa acctggcgca ataacgccag cgcattcagg tcgtaaagtg cggagagatc
    18301 gcccacaatc gccagcgtcg gtttgccgct tgcccgctga acgccggcgg cagtcgagag
    18361 cagcccgtcg ataccgctgg ccccacggtt gctgtacacc gggtaacctg ccggaagttg
    18421 cgaaagcgca tcaatcagac gtaccaccag gctgttaccg acaaacaatt gcccctgttc
    18481 aggcagatag tcgctgatgc gatgcgccag ttgcgcttcg ccaaacgcat cgcgacaggc
    18541 aataaccgcc tgcattgcct gttccgccag gcgcgggatt tcaacgcacc agggctggcg
    18601 tttttctgcc ggatgcagtt ccagccagtc ggcaatattg gcaattaagc ggcgtccacg
    18661 atggtgtgcc ggatcaagcc gcccttcgat gtcatccaca atccagtact cttcgggttc
    18721 acagcttgcc tgccattgca ggagccgttt gcccgtcagg ctgcttccca gttgcaccac
    18781 aatttgcgcc tgttgcagtt cgctggtcgc tttggcattg cctaaccaga gatcggcaca
    18841 cggcagcggc tgtccggttt gcgacagcac gtcgccaatc agcggccagc caagagtttg
    18901 cgcccacagg gcaacttttt tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc
    18961 gcgcttttgc cgccagaaga accagtcgcg ctgtttttcg ctctcaagac gaggcgcttc
    19021 acgcagccac ggcttatcat cctgccacca gtcacccaga cgctgttgcc agctaagccc
    19081 agtgtcgtcc atctcgccat acagcggttc agcaaacggg cagttgatat ggaccccacc
    19141 agcatgaagc gtaccgagag catggtcgat ggtagaaacc agccaacgtg cggggatatc
    19201 ctgggtcggg cgcggcagtg aaatactgtg cgtggggtga gaggcgaaca ttcccggctg
    19261 acgaatcgcc tgattcgcgc cgcagtcaat tagctccggc gggcgatcgg cggttaacag
    19321 gatcagtttt tctccggtta acccagcttc aatcagtgcc ggatagagat ttgctaccgc
    19381 cgtgccggag gtcacaatca ccgccaccgg ctgcttgctg actttcgcca gccccagcgc
    19441 cagatgcccc agtccacgct catcgaaatg ggtgtggtga atgaatgcgg aattctccgc
    19501 cgccgctaac gttaacggtg tagaacgcga gccaggggcg atgcagatgt gtcttacgcc
    19561 gtgacgcgtt aatgcttcca gaatgaccgc cgcccagcgt cggttaaatg cgcttactga
    19621 catgagtttg tccggtatca atattgcggc taagtataag gagctacaaa aatagattat
    19681 tgatatgaat cggtaatgat gcgactcatt actattccat ttgtaataaa gtacgcagcc
    19741 ctgccgcttt gttgtcgatt tcctgccact cttgctcggg gtcggaacca cggacaatgc
    19801 ccgcgccagc atatagccgc acgacattgc cgctgatttt tgctgagcgc agggaaacgc
    19861 agaattcgct ttgttgtaat gagagatagc ccgcagaacc ggcgtaccat tctcgggtga
    19921 acggttcgtg atgggcgata aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg
    19981 ccgttggctg caactgatgt aaacagatca catcatccgc tttgttgagt gaagtccaga
    20041 tacagcggcg aagatgctgc actttacgca gacgcagtac ctgcggcggt aaaacatcca
    20101 gcgtctgggt atcggcctgt aatcgttggc agatatcttc caccaccagc atgttctcgc
    20161 gctggttttt atcatccgcc atcagccact ctcctaactg ctgcgcctgc ttatcatcag
    20221 gatgatttgc tactgttccc gccagcgctt cagtacgcag cgctttgtca cgccgccgcc
    20281 ataaccgttc cggtgaagag ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat
    20341 ggtagcaatt gagattcagt cgacgactgg cagccatcat cgccgccgca ttgaccggac
    20401 ttgcgaaatg caggtcagtt gcccgagcaa gcaccacttt gtcgagctca ccttcggcga
    20461 tggttttcgt tgccagttcg attaattgcg tccagcccgt tttgtccggc cagtgttgtt
    20521 ctcgcgtagt ggttaaatgt aacccaggca agggcttgat actcaccagt gtggcgataa
    20581 attctcttgc cttaatcgca tcgtgctgaa gggagctttc gctgaatagc gtcagccgca
    20641 gcgtggcttt accgccacag cgtcgccatt ccaggcgtgg taaaagtaaa ttgccctgcg
    20701 acgggtcaaa cgcattcagc ccccaaatgc gtaagtcggc gtgttccggg tgctggcgaa
    20761 gaaaacgttg tgcctggtcc aacgacgtaa aacgggtaat cgcgcccagg acggcagctt
    20821 cttcatcacc attacgttgt tgccagtaga attgcgggta cgtttgctga ctggccagcc
    20881 agctcaaggc atcaaaagcg tctttgagag ggaaaggaat atcgataacc cgaatgccgg
    20941 gtgtcgccgg aatctcttgc gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg
    21001 attgcacgcg gacctctccc tgttaaaagc ctcacattat acggggtact acaaaaaaaa
    21061 tgcagtaccc cggtgtaggg aggttttaac ggcgtgccag caacagtcct agtaccagcc
    21121 caacggccgc acccacacca attccctgcc agggtttttc gtggacgtag tcatcagcac
    21181 gataaacagc ctgcttcgcc cgatagtaat aactgtctga agcctggcta acccgttttt
    21241 tcacatcatc cagcgctttt tctgcacgcg ctttcagctc aacatatttc tgatcggcgg
    21301 gatcgccaga ggagcggagc acctcttcca gtgtttcact aagcagcgtc aggtcgtcat
    21361 cgatacgtgt atcaccaaac tgattagaca ttctcgttct ccatttgcgt aaaacctgtc
    21421 actaactata gacaagggtt attgattacg cctgaattac ctcgcgtgcc atcccaatgt
    21481 gtgggatacc atcttcttca tagatctccg tcacagggat aaaaccgaaa ctctggtaga
    21541 agttttgcag atgcgcctgc gccccgaggt agacaggctt atcaggccag tgatgcgtgc
    21601 aagtgtcgag ggttttactc atcagttgct gccccacttt ttcgccacgc aacgcttcac
    21661 tgacaatcac ccgaccaata acgaccggct caagatcatc atcacttttc agaatcctcg
    21721 catatgccac cagttcatca tttttccaac cgagaatatg gcgattatcc cccgtcaaat
    21781 catcaccatc gatatcctga taagggcaat tttgctcgac aacaaacacc gcgcaacgca
    21841 gttgtaataa ggcataaagc tgggaaaccg ataattcaga gtgatgcaga tcttgccatt
    21901 caatcataat gctctccttt tagatgtatg ccttatacta gccggaatat tttttgaaac
    21961 gtgatgaact catggaatta atttttttag gtacttcggc cggtgtgcca acccgcacgc
    22021 gcaatgtcac ggcaatattg ctaaacctgc aacatccgac ccagagcgga ctttggttgt
    22081 ttgactgcgg tgaaggcacc cagcatcagc tactgcatac cgcctttaac cctggaaaac
    22141 tggacaagat ttttatcagt caccttcatg gcgatcatct ttttggttta cccggcttgc
    22201 tgtgcagtcg ttctatgtca ggcattatcc aacccttaac gatttatggt ccgcaaggta
    22261 tccgtgaatt tgtggagacc gcgctgcgga ttagcggctc atggaccgat tatccgctgg
    22321 aaattgtcga aattggcgct ggcgaaattc tcgatgatgg cctgcgcaaa gtgaccgctt
    22381 atccaatgga acacccactg gaatgctatg gctatcgtat tgaagaacat gataaaccgg
    22441 gtgcattaaa tgcccaggca ttaaaagctg ctggcgtacc gcctagccca ctgtttcagg
    22501 aattaaaagc gggcaaaaca atcatgctgg aagatggaag gcagattaac ggcgcagatt
    22561 acttagctgc tccagtgcca ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg
    22621 atgccgcact tgacctggct aaaggtgtcg atgtcatggt gcacgaagcg acgctggata
    22681 taaccatgga agccaaagcc aatagtcgcg gtcatagctc tacacgccag gctgcgacac
    22741 tggcccgtga ggctggagtc ggtaagctaa tcattaccca cgtcagctcg cgctatgatg
    22801 acaaaggttg tcagcacctg ttacgtgaat gcaggtcaat tttcccggcg actgaactag
    22861 cgaatgattt caccgtgttt aacgtttaac tctgcttatt attaaaaaca gggcgaaact
    22921 tgccctgtta tcgcaacccg cgctacatat atccactcac acgggcatat tgagcgattt
    22981 ttgatattta ttcatctcct tacgcactat attcagagca ataagttgcc tctttttatc
    23041 ttcattcagc acaacctttg caactaaaag gagcatatga tggttacagt tgtcagcaat
    23101 tattgtcaat tatctcaaac ccaactcagt cagacatttg cagaaaaatt tactgtgacc
    23161 gaggaattac tgcagtcttt aaaaaaaaca gcgttatccg gagatgaaga aagcattgag
    23221 ttactgcata atattgcgtt aggttatgat gaatttggga aaaaagctga agatattctt
    23281 taccatattg ttagaaaccc aacaaatgat accctatcga ttatcaagct tataaaaaat
    23341 gcctgtttaa a
//
LOCUS       DAZKIF010000079        22818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZKIF010000079 DAZKIF010000000
VERSION     DAZKIF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.079"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..95)
                     /locus_tag="ACHOTW_002961"
     CDS             complement(<1..95)
                     /locus_tag="ACHOTW_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845024.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQ"
     gene            complement(171..788)
                     /gene="yncG"
                     /locus_tag="ACHOTW_002962"
     CDS             complement(171..788)
                     /gene="yncG"
                     /locus_tag="ACHOTW_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP9845025.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEII"
     gene            1055..2554
                     /gene="ansP"
                     /locus_tag="ACHOTW_002963"
     CDS             1055..2554
                     /gene="ansP"
                     /locus_tag="ACHOTW_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP9845026.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2669..3730)
                     /gene="yncE"
                     /locus_tag="ACHOTW_002964"
     CDS             complement(2669..3730)
                     /gene="yncE"
                     /locus_tag="ACHOTW_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP9845027.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3972..6074
                     /gene="pqqU"
                     /locus_tag="ACHOTW_002965"
     CDS             3972..6074
                     /gene="pqqU"
                     /locus_tag="ACHOTW_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP9845028.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            complement(6110..6775)
                     /gene="mcbR"
                     /locus_tag="ACHOTW_002966"
     CDS             complement(6110..6775)
                     /gene="mcbR"
                     /locus_tag="ACHOTW_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP9845029.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6973..8010)
                     /gene="curA"
                     /locus_tag="ACHOTW_002967"
     CDS             complement(6973..8010)
                     /gene="curA"
                     /locus_tag="ACHOTW_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP9845030.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8191..8709
                     /gene="mddA"
                     /locus_tag="ACHOTW_002968"
     CDS             8191..8709
                     /gene="mddA"
                     /locus_tag="ACHOTW_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP9845031.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8706..9155
                     /gene="ydcZ"
                     /locus_tag="ACHOTW_002969"
     CDS             8706..9155
                     /gene="ydcZ"
                     /locus_tag="ACHOTW_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9845032.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9156..9389)
                     /gene="ydcY"
                     /locus_tag="ACHOTW_002970"
     CDS             complement(9156..9389)
                     /gene="ydcY"
                     /locus_tag="ACHOTW_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP9845033.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9475..9648)
                     /gene="ortT"
                     /locus_tag="ACHOTW_002971"
     CDS             complement(9475..9648)
                     /gene="ortT"
                     /locus_tag="ACHOTW_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP9845034.1"
                     /translation="MSLYQHMLFFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9843..9938
                     /gene="yncL"
                     /locus_tag="ACHOTW_002972"
     CDS             9843..9938
                     /gene="yncL"
                     /locus_tag="ACHOTW_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP9845035.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10035..11459)
                     /gene="patD"
                     /locus_tag="ACHOTW_002973"
     CDS             complement(10035..11459)
                     /gene="patD"
                     /locus_tag="ACHOTW_002973"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP9845036.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11481..12275)
                     /gene="ydcV"
                     /locus_tag="ACHOTW_002974"
     CDS             complement(11481..12275)
                     /gene="ydcV"
                     /locus_tag="ACHOTW_002974"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9845037.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(12265..13206)
                     /gene="ydcU"
                     /locus_tag="ACHOTW_002975"
     CDS             complement(12265..13206)
                     /gene="ydcU"
                     /locus_tag="ACHOTW_002975"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9845038.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVTQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(13207..14220)
                     /gene="ydcT"
                     /locus_tag="ACHOTW_002976"
     CDS             complement(13207..14220)
                     /gene="ydcT"
                     /locus_tag="ACHOTW_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9845039.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(14238..15383)
                     /gene="ydcS"
                     /locus_tag="ACHOTW_002977"
     CDS             complement(14238..15383)
                     /gene="ydcS"
                     /locus_tag="ACHOTW_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP9845040.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15628..17034)
                     /gene="ydcR"
                     /locus_tag="ACHOTW_002978"
     CDS             complement(15628..17034)
                     /gene="ydcR"
                     /locus_tag="ACHOTW_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9845041.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(17113..17529)
                     /gene="hicB"
                     /locus_tag="ACHOTW_002979"
     CDS             complement(17113..17529)
                     /gene="hicB"
                     /locus_tag="ACHOTW_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP9845042.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17575..17751)
                     /gene="hicA"
                     /locus_tag="ACHOTW_002980"
     CDS             complement(17575..17751)
                     /gene="hicA"
                     /locus_tag="ACHOTW_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP9845043.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            17973..18203
                     /gene="yncJ"
                     /locus_tag="ACHOTW_002981"
     CDS             17973..18203
                     /gene="yncJ"
                     /locus_tag="ACHOTW_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFP9845044.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(18295..20256)
                     /gene="rlhA"
                     /locus_tag="ACHOTW_002982"
     CDS             complement(18295..20256)
                     /gene="rlhA"
                     /locus_tag="ACHOTW_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP9845045.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(20329..20865)
                     /gene="sutR"
                     /locus_tag="ACHOTW_002983"
     CDS             complement(20329..20865)
                     /gene="sutR"
                     /locus_tag="ACHOTW_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP9845046.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            20918..22129
                     /gene="ydcO"
                     /locus_tag="ACHOTW_002984"
     CDS             20918..22129
                     /gene="ydcO"
                     /locus_tag="ACHOTW_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP9845047.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            complement(22164..>22818)
                     /gene="ydcL"
                     /locus_tag="ACHOTW_002985"
     CDS             complement(22164..>22818)
                     /gene="ydcL"
                     /locus_tag="ACHOTW_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP9845048.1"
                     /translation="FAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKETTSATG
                     KPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIAQ
                     RKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRLY
                     FEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
BASE COUNT         5557 a         5849 c         5590 g         5822 t
ORIGIN      
        1 atctgcacaa tgtgaatagt tagtttcttt tcagaggaaa tgaaatagac aagctgaatg
       61 aaagggagtg tgatataaat taaaaaacat aacatgaatc taattaatta cgcacatcat
      121 tgtggataaa gttactgaat aataaataac ataatcttct ggtatcccac tcaaataatt
      181 tcattccttt ttagtatttc ttgtaacgtt ggtaactgac atactgcatc agcaatcgca
      241 ctgatattcg tggcattatc ctgaaaccat tcatgcccag gcccccatgt gcgcatagtg
      301 caaagataac agtccaccag cgttagttgc tcaccaaacg cataaggttc agcggtcaat
      361 tgtgaattca gccagacata aagcgattta cgatattcaa taacattctt ctttaactgt
      421 tcaggtgcgt cagatgccca acgctcaggg taatcggcga aagtgaacgt tggatagaca
      481 ttagcaacca gccagaccaa taaccgttga aactgctgac gttcggcacg cccaaccggt
      541 ggcgcaaggt ccgggcgacg atcgagaacc atcaatgcaa tcgccgctgt ctccgtcata
      601 atctcgtcat tttccagtgc cagtgtcgga acctgacaca acgggttcag ggttttcaat
      661 aactcgcgtg aagttccctc gtggtcaaaa ccactgacat caacgaactg ataaggaata
      721 tcagccagtg ttagcatcag ctcactgatt gtagagcccc atccgggtac gccatacacc
      781 ttaatcatat gcccctcaca atcttttaaa gattaaatgt aggacagtac aaaggttaac
      841 cagcaaaagg atttgtcatt ttttcgtcag ttgtattaat ccccttcagg attgtaaggg
      901 gatgttttgc atcgtttatc gataacgggt aggatgctgc gccgatagtt ttatacccat
      961 aaagaataat ggtgataact atcatcgcca ggatgaataa acattgttca tggcaactta
     1021 tatgactttt tcattaaagc aatcagggag agcaatgagt aaacacgaca ccgacacttc
     1081 agatcaacac gccgcgaaac gccgctggct taatgcccac gaagaggggt atcacaaagc
     1141 gatgggcaat cgccaggtac agatgatcgc cattggcggc gcgattggca ccggcttgtt
     1201 tttaggtgca ggagcccgac tgcaaatggc gggtccagca ctggcactgg tttatttaat
     1261 ttgtggcttg ttttcgtttt ttattctgcg tgcattgggt gagctggtgc tacaccgccc
     1321 ttccagtggc agttttgtct cttatgcccg tgagtttttg ggtgagaaag ccgcttatgt
     1381 tgctggctgg atgtacttca tcaactgggc gatgacgggg attgttgata ttaccgccgt
     1441 cgctctgtat atgcattact ggggtgcgtt tggcggcgtg ccgcagtggg tctttgcgct
     1501 cgctgcactt accatcgttg gcaccatgaa tatgatcggt gtgaaatggt ttgcggagat
     1561 ggagttctgg tttgcgctta ttaaagtgct cgccattgtg acctttttgg tcgtgggtac
     1621 ggtgttcctc ggtagtggtc agccgctgga tggcaacacc actggctttc atttaatcac
     1681 cgataatggc ggcttcttcc cccacggttt gctgcctgcg ctggtgttga ttcagggcgt
     1741 agtgtttgct tttgcctcca ttgaaatggt gggtacagct gccggagaat gtaaagatcc
     1801 gcagaccatg gtgcctaaag cgattaacag tgtgatttgg cgtattggcc tgttttacgt
     1861 cggctccgtg gtgttgctgg ttatgttatt gccgtggagc gcgtatcagg cggggcaaag
     1921 tccgttcgtg acgtttttct ctaaactggg tgtgccatat atcggcagca ttatgaacat
     1981 tgtggtgctg accgctgccc tctccagcct gaattcaggt ctgtactgca ccggacgtat
     2041 tctgcgctca atggcgatgg gcggttccgc accgagtttt atggcgaaaa tgagtcgtca
     2101 gcatgtgccg tatgccggga ttctggcgac actggttgtg tatgtcgtcg gcgtattcct
     2161 caactatctg gtgccgtcgc gcgtatttga gattgtgttg aacttcgcgt cgctgggaat
     2221 catcgcttca tgggcgttta tcatcgtgtg ccagatgcgc ctgcgtaaag cgattaaaga
     2281 aggcaaagca gcggatgtca gttttaaact gcctggcgcg cccttcactt cctggctgac
     2341 attactgttt ttactgagtg tccttgtgct gatggcgttc gattacccga acgggactta
     2401 tactatcgcg gcgctgccga ttatcggtat tctgctggtt ataggctggt ttggtgtgcg
     2461 caaacgcgtt gctgaaattc acagcactgc gccagtcgtc gaggaagatg aagaaaaaca
     2521 ggaaattgtg tttaagcctg aaacggcgag ctaattgcaa aattgcccgg ctacgtgccg
     2581 ggcaaattta gattactgac gaaggtgtgc cgcatgcggc gtgatcaaag tcaggcaagt
     2641 ctctccgtta tcaccctcgc catatctatt acagcgcaat acgaatcaca tcgtctggct
     2701 gggtagcttc ctgctgttta gtggattttt gtttcacact gacatacagc gttttgccat
     2761 cggcagacag cgccaggctg ttcggatgag tcggcgtatc gaacgttttc accactttat
     2821 agcttttcgc gtcaatcaca ctgactttac ctgcctgacg atgcgttacg taggcttcat
     2881 tacgcgctgg gttaaacagc acagccagtg attccggtgc cgcaaccttc gccagaatat
     2941 tgccattacg ggtatcgacc actaacactt ctgccgcttt agaatcggtg ataaatgcac
     3001 gctgcctggc ggtatcaagg ctgatgttga taaagaagtg ctctttgccg tcatccagca
     3061 gctttttacg gctgaggatt ttattgtcgg cggtgtcgat ggtaatcaat tcgccgtcag
     3121 cgttagtggt gtaaagacgt ttgcctttgc tatccagtgc cagaccggta ctcattttac
     3181 cggtgttctg gatggcggtt ttcagtttga tattctcgcc atcaacgacc caaatcacgc
     3241 tatctttacc aataccactg atgtaaacgg tgttcgtggc atcatcagca accagctcac
     3301 gcggttgcag cgggcgcacc tcttccgtgc gcttacgatc atccagcacc agacggcctt
     3361 tcacctcgcc agttttggca tctatcgccg tgaccgcgct gtttacggtg ttaccaaacc
     3421 acaacgtctg agtcgtgtta ttgatggtgg caccaaacgg cttgagatcg ttatggatcg
     3481 cctgcgtcac ttccagagtg accggatcaa gacgataaac cacgccgcct ttatccagtt
     3541 tgcggctttg cgaagtggcg agccacagcg cgttttcttg ctggctataa gccatttcgt
     3601 aggcaccttt acctaccgct ttacgcagca tttcttctgc ggcctgcgta ctgaatgatg
     3661 aagcaacaag caatgaacct aacagtaatg aaccacgcag gcgcaatgaa aacagatgac
     3721 gtaaatgcat gacgactccc tttgatgaac cgatattttt gctgcttcac aatgcttcgc
     3781 taagcgtaat catcgttacg ctcttgttat aaatgggaat ggtaatcatt atttactcaa
     3841 atgtggagca tttcttgccg ttacgactac aattctctca attaattaac gtatcgtgcc
     3901 gtttcgtttt ccataaattt acaatttcat taccatgacg gtgttttccc attcttaacc
     3961 tggctttttt catgaagatt gttttcgttc gacagaccgt tttgcccgcc ctgcttgtcc
     4021 tttcccccgt tgtttttgcc gctgatgaac agaccatgat tgtcagtgcc gcgccgcagg
     4081 tggtttcaga actggatacc cctgcagcag taagcgtggt ggatggcgag gagatgcgcc
     4141 tggcaacacc gcgcattaac ttgtccgaat cactgaccgg cgtgcctggt ttgcaggtac
     4201 aaaaccggca gaactatgcg caagatttac agctgtcgat tcgtggattt ggctcccgct
     4261 ccacttacgg aattcgcggt attcgcctgt atgtggacgg tattcccgct accatgcccg
     4321 acgggcaagg gcaaacatcc aacatcgatt taagcagtgt gcaaaatgtg gaagtgctgc
     4381 gtggcccctt ctctgccctg tatggcaacg cgtctggcgg tgtaatgaat gtcaccaccc
     4441 agaccgggca acagccacca accattgaag ccagtagtta ctacggcagt tttggcagct
     4501 ggcgctatgg gctgaaagca acgggcgcaa cgggagacgg cacacagcct ggcgatgttg
     4561 attacaccgt ctcaaccacg cgttttacga cccacggcta tcgtgaccat agtggcgcac
     4621 agaaaaattt agctaatgcc aaactgggcg tacgcattga tgacgccagc aaattaagcc
     4681 tgattttcaa tagcgtggat atcaaagcag atgacccagg tgggctaacc aaagcagaat
     4741 ggaaagcgaa tccgcaacaa gcgcctcgtg cagaacagta cgacacgcga aaaaccatca
     4801 agcaaactca ggctgggttg cgctatgaac gtagcctgag ttcgcgggat gatatgagtg
     4861 tgatgatgta tgccggagag cgagaaacga cccagtacca gtcaataccc atggcaccac
     4921 aacttaaccc gtcacatgcg ggcagcgtga ttaccctgca acgccattac cagggaatag
     4981 acagccgctg gacacaccgt ggtgaactgg gcgttccggt cacgttcact accggcctga
     5041 actacgaaaa catgagtgaa aaccgcaagg gctacaataa cttccgcctg aatagcggca
     5101 tgccggagta cgggcaaaaa ggtgagttgc gtcgcgacga acgcaatctg atgtggaaca
     5161 tcgatcccta tttacagacg cagtggcagc tgagcgaaaa actgtcgctg gatgctggcg
     5221 tgcgctacag ctccgtgtgg tttgattcca acgaccatta cgttactccg ggtaacggcg
     5281 atgacagcgg tgatgccagt tatcacaaat ggctaccagc cggatcgtta aaatatgcaa
     5341 tgaccgatgc ctggaatatc tatctggcag ccgggcgtgg ttttgaaacg ccgacgatta
     5401 atgagctgtc ttatcgcgct gatgggcaaa gcggtatgaa ctttggttta aaaccatcca
     5461 ccaacgatac aattgagatc ggcagtaaaa cgcgtattgg tgatgggctg cttagtctcg
     5521 cattgttcca gaccgacacc gatgatgaaa ttgttgtcga tagcagtagc ggtgggcgta
     5581 cgacttacaa aaatgctgga aagacccgtc gtcaaggcgc tgaactggca tgggatcaac
     5641 gtttcgcggg agattttcgc gtaaacgcgt cctggacctg gcttgatgcg acctatcgca
     5701 gcaatgtttg caacgaacag gattgtaacg gtaatcggat gccagggatc gcccgtaata
     5761 tgggctttgc gtcgataggt tatgtaccgg aagatggttg gtatgcaggc atggaagcgc
     5821 gttatatggg cgatattatg gcagatgatg aaaatacggc caaagcgccg tcttatactc
     5881 tcgtcggctt attcaccggg tataaataca attaccacaa tttaactgtg gatttgtttg
     5941 gtcgtgtcga taatttattc gataaagaat acgttggttc tgtcattgtc aatgagtcaa
     6001 acgggcgata ttacgaacct gcgcccgggc gaaattatgg tgtcggcgtg aatatcgcat
     6061 ggcgatttga gtaattacct tgatgcccgg tatttgccgg gcatttactt taacgattgt
     6121 attgctggta taaaatagca atattttgtt gcataatttc agcaagacaa tgtctgcatc
     6181 cctctttgtc ttttgctttt aacgcagcga gtaacagatg atagttttct atatgctccc
     6241 gtaattccgc tggattaatc gcttcataga gataatgtaa accaggtccc atcctgaccc
     6301 acagttgctc aatcatctca cacaggatgg gcatatttga gcgatgataa atcgctaagc
     6361 gaaatagcct gtttacatta atgatttgtt ccatgtcgcc cttttcttgc gcctgctgta
     6421 atttctcgag caattcctgg agttccgcca ggtcttgcgg ggtgaggttt tcaacagcca
     6481 gagcaactgc cattaattcc agttcgtagc ggatccgatt gatttcgtct aattgacgtt
     6541 tccccacttc cggaactgta aatgcttgcg ctggtgcgac agaaagcgca ttcaccgaaa
     6601 ccagacgtaa taatgcttca cgcacaggtg taatactcat acctaattgc tccgccagat
     6661 ttttagtaat caggcgtgcg ccaggtttta gtgcgccaat actaagctga tgtttcaggt
     6721 cgttctcaac ctgcagagtc aaactgacat gtttcatttt ttccgttcca ggcattttaa
     6781 ttccttcgcg tgttttctgt ccaaccctgc ctgcatggca gaaggaattc acctggtgta
     6841 ttaaagtgaa taatcataaa ccactcacct ttgttgttat tagcagcaga acaatgatgt
     6901 ggttagttaa ttaaagctga aaataagctt tttgcctgga tttttatggc ggcttacgcc
     6961 gccgttgaaa ctttaatcat cacccgccac gcggatcacc actttgccga agtttttacc
     7021 cttcagcagg ccgataaacg tctgtggcgc attctctaaa ccgtcagtaa tttcttcgcg
     7081 gtagtggatt ttatcctctt tcacccattg ccccatctcc ctctgaaact catggatgcg
     7141 gtgaccataa tcctgagcga taataaaacc ttgcaagcga atacgttttt tcagcactgt
     7201 agccatcaac agaggtaaac gatccggacc gggtggtagc tctgtagcgt tatagctgct
     7261 cactaatccg cagacgggaa tgcgcgcaga tgtattaagt aacggtagca ccgcatcgaa
     7321 taccttaccg cccacgtttt cataatagat atcaatacct tttgggcacg ctttcgccag
     7381 ttgttcggca aaatcatccg cgtggtgatc aagacaaaca tcgaagccta acacctcggt
     7441 agcatggcgg catttttccg cgccaccggc tacccccacc actctgcaac ctttaagttt
     7501 gccgatttgc cccaccgtcg cccccactgg tcctgtcgcc gcagctacca ccaacgtttc
     7561 gccctcttta ggctgaccga tatccagtag gcccatataa gcggtaaagc ctggcatccc
     7621 tagcacaccc agcgaccacg atggattttg cggatgatcg ccaagtttca ccagatcatc
     7681 accactggat atgtcatagt cttgccatcc actgtagccc agcacccagt cgccagactg
     7741 ataatcagga tgattcgact ccacgacacg gctcaccgta ccgccgacca tcacgccgcc
     7801 aatatcaacc ggcggtgaat aagatggctc atcgctcata cgtccacgca tatacgggtc
     7861 cagggacaaa taaactgtgc gcagtaacac ctgtccttca cccggtgtgg cgacatcatc
     7921 ttcttcaaga cggaaattct ccggaaccgg tgcgccatgt ggacgcgagg ccagaaccca
     7981 acgtcgatta cgctgctttt gttgccccat aatgctctcc tgtgcttttg gcatgaatcc
     8041 atcaatagta gcaggctcgc aggattggtt ttgtttgcag ccgtcacccc ccctctgcaa
     8101 cacgcaataa tttcatcgag gaatccgttg cttcgggctg ttttcgatag tattgataac
     8161 atcatcatca ttattttatt gaggccagac atgtccatcc gttttgcccg caaagccgac
     8221 tgtgctgcca ttgcggaaat ttataaccac gccgtgttgt atacggcggc tatctggaat
     8281 gaccaaacgg tggatgcaga taaccgcatt gcctggtttg aagcgcggac catagcaggt
     8341 tatccggtgc tggtaagtga agaagatggc gtagtgacgg gatatgcctc atttggcgac
     8401 tggcgtagtt tcgatggttt tcgccatacc gtggaacatt cggtttatgt ccatcccgat
     8461 catcagggca aaggtctggg gcgtaaattg ttaagccgat tgattgatga agcgcgagat
     8521 tgcgggaagc atgtcatggt cgccgggatc gaatcgcaaa atcaggcctc gctgcatctg
     8581 caccagtcgc tgggatttgt cgtcaccgca caaatgccgc aggtgggcac taaatttggg
     8641 cgctggctgg atctgacatt tatgcagttg caactcgacg aacgcactga accggacgcg
     8701 attggatgaa tcagtcgctc acccttgcct ttttgattgc cgccggaatt ggtctggtag
     8761 tccagaactc gctgatggtg cgcatcaccc agacctcctc taccattctc atcgccatgt
     8821 tgctgaactc actggtgggg attgtgctct ttgtttctat tttatggttt aaacaaggta
     8881 tggcggggtt tggcgaactg gtgtccagcg tgcgctggtg gacacttata cctggcttgc
     8941 tgggatcgtt ttttgtcttc gccagtatca gcggatatca gaatgtggga gcggcgacaa
     9001 ctattgcagt actggtcgcc agccagctga ttggcggtct ggttctggat atcttccgca
     9061 gtcatggcgt accactacgg gcgttgttcg gcccaatttg tggcgcaatt ttacttgtgg
     9121 ttggcgcctg gttggtagcc agacgctcat tttaatcaca ggatggtgcc gcctttggtt
     9181 aactgttcat ggcgcgcttc catatcttct ttatgcttgc gaccgtgatg agcaatagcg
     9241 gtgcgcaaac gctgctgctg ggtgtagcga tcttcccgac ttaactccgc gtcatcgctc
     9301 agggcgatta ataattcgtt catatgggca atgacgctct cttcaatcgc ggcatccacg
     9361 cgcgcgatga cttcatccag atgcgacata ctttctcctt agccacttgt gaaatttatc
     9421 gattcatcca aatgatataa gtctgcgtgg tgccgtcaag ccagtgagta agatttaaaa
     9481 cagtgaaaac aacagcgcca ccgggaaact catcggccat gttgccccca ccagaaaggc
     9541 gcttaagaaa cggatgcgtt tcttatcgtg agaaagaaac caagtgataa gaaatgcgat
     9601 tgctgccata accgcataaa aaaaaagcat gtgttgatag agagacatgt acgggcgtcc
     9661 agccagatga ataaaacgcg cgcaaatatg cgtcattttt tgacgtgcat caagtttttt
     9721 aaccggtttg agaaaaaaac atcaaacagc aacttgccct tatccatctt cagaatgagt
     9781 attattgcgg attttcttaa cccgtactat acacataggg gcaatgataa aaggtggcaa
     9841 aaatgaatgt ttccagtaga actgtagtac tgataaatgt ctttgctgct gttggtttgt
     9901 ttactcttat ctctatgaga tttggctggt ttatttgatg tagtgatttt gcacgcagca
     9961 cgtactgtca gagaaatcag tgctctgaca gtacgcaaga tcatctcata ctcttacgat
    10021 ctgcaatatt atccttaatg tttaaccatg acgtggcgga cgacggtgta atcctccagc
    10081 ccataaagtg acatatcctt gccgtaacca gaaagtttct gcccaccgtg cggcatttca
    10141 cttaccagca tgaaatgggt attgacccag gtacaaccat attgcagccg tgcactgacg
    10201 cgatgcgccc tgcccacatc tttcgtccat accgaagatg caagtccgta ctggctgtca
    10261 ttcgcccagt ttaccacctg ttcttcgttg tcgaagagcg taacactcac tactggacca
    10321 aatacctctt tttgcacgat ggcatcgtcc tgtaatgcgc cagccagcag cgtcggcgca
    10381 tagtaatagc cattaccctt gcgcttttca ccgccagtga tcactttgat gtgccctgtc
    10441 gctttcgcct cttctactgc cttgctgacg cgttcgagat gcgccagcga gcttaaaggt
    10501 ccaagctccg tagactcgtc atccggcgca ccagatttta acgttgccac tgcagcaccg
    10561 agtttttcca ccagcgtatc gtaaatgcct ttttgcgcgt agatccgaca agccacagta
    10621 caatcctgtc cggcattgta atagccaaat gtacgtacac cttcgaccac tgcttcaata
    10681 tccgcatcat caaaaacaat cactggcgct ttgccaccaa gttccatatg agtacgctta
    10741 atggagggcg cggtatggct gatgatgtgc tcgccggtgg cgatagagcc cgtcagcgac
    10801 accatccgca ctttgggatg accggtcagc ggatcgccca ccgttttgcc tctgccaaac
    10861 agtacgttaa tcacgcctgc cgggaagata tctttcgcca gctctgccaa cttcaacgcg
    10921 gtcagcgggg taatttctga tggtttaagc actacgcagt tccctgccgc cagcgccgga
    10981 gccagtttcc acgctgccat catcagcgga taattccacg gtgcgataga agccacgacc
    11041 cccaacggat cgcgacggat catcgaagta tgaccttcaa gatattcacc tgccgccaga
    11101 ccattcagac agcgcgccgc acccgcgaaa aagcgaaaaa catcgacaat cgccgggatt
    11161 tcatcattga acgcactatg cagcggtttg ccacaattac gggactccag ttcggcaaaa
    11221 acctgaccat tttcttcgat aacatcagcc agtttcagca gacattccgc acgcactttc
    11281 ggcgtggttt gcccccattc ggcaaatgct gcatctgccg cgcgcacagc agcatcgacc
    11341 tgctctgcgg atgcctcggc aatctccagt aaaacgtccc ccgttgccgg attatagaca
    11401 ggctgttttt ccccttcgcc gctaaccagt tctccgttaa tcagtaactt atgttgcata
    11461 gcatattcct gtatcagtgg ttattgacca ttgtcgcctt cgcgggttag ccaccaggct
    11521 cccaggatcg gcaaggttgt taccaacata accagcagtg ccaccacgtt agttaccggt
    11581 acatcacgcg gtcgcccaag ctgattgagc aaccacaacg gtaacgttcg ttcatgacct
    11641 gccgtaaagg tcgtaacgat gatttcatcg aacgacaagg caaacgccag cattcctcct
    11701 gccagtaacg ccgaactgag attcggcaac actacgtagc ggaaggtttg ccagccattg
    11761 gccccaagat ccattgacgc ctcaaccaga ctccatgagg tgcggcgaaa acgggcgatg
    11821 acattgttaa acaccacaac tacacaaaaa gtcgcatgac cgaccacgat ggtgaaaaat
    11881 cccggctcca gattgatggt tttaaaggcg gttaataacg ccagaccagt gacaatgccc
    11941 ggcagcgcaa tgggcagcag cagtaacagc gaaatggcgt ttttgccgaa aaagtctcgt
    12001 cgccacagcg cggcagctgc cagcgtccct aacaccagcg ccattaatgt cgccagcgcc
    12061 gccactttaa gtgacagtgt cacggcatca agaatatcac tacgctgtgc tgccacgcta
    12121 aaccagcgca gcgtcaggcc ctgcggtgga aaactaaacg ccgcatcttc agtgttaaac
    12181 gcataggcgg cgattatcag gatgggaaaa tgtaggaaaa caacgccgcc ccaggccgcc
    12241 agtttgagga aaaacggtgc gcgttcagag tgcatcaaac gctcccagac gtttcacgaa
    12301 cgccaggtac agtgcgataa gaacaatcgg caccagggtg aatgccgccg ccatcggcat
    12361 attgccaatc gccccctgct gggaataaac catattgccg ataaaatatc ctggcgggcc
    12421 aaccagctgc gggacgataa aatcgcccag tgtgagtgag aaggtaaaga tagagccagc
    12481 ggcaataccc gggatcgcca gcggcaacac cacatagcga aaggtttgtc gtggacgtgc
    12541 gccgagatca gctgacgcct gcaacaatga cggcggcaaa cgctcaagcg ccgcctgcac
    12601 gggcaggatc atgaacggca gccagatata gagaaacacc agaaagcgcc ccagcccgga
    12661 agttgacagc gtattgccgc ccaccgcagg taatgtaagg aacgctgtca gcagtggttc
    12721 cagcccaaga tgttgtaaaa accactgagt cacgccatct tttgccagca ataacgtcca
    12781 ggcataggct ttaacaatgt agctcgccca catcggcaac attaccgcaa tataaaaaaa
    12841 cgctttcatt ttcccgctgg tatagcgcgc catataccac gccattggaa aagccagaat
    12901 ggcgctggcg atagtgactg ccacagccat agtcagcgtg cggagaatga tgtcgtaatt
    12961 cgccggatta aacagcgcac ggatgttcgc cagcgtcagt tccggcgtta ccgacatggt
    13021 gaaatcgtca aaggtataaa agccctgcca taacagcgtt agcagcgagc cgaaatagac
    13081 aatgccaaac cacattagcg ggccaagcag cagcaaaaac agccccagcc ccggattccg
    13141 ccagaagaaa ccggacacct tacccagacc tggacgtgaa ggtgattgca atacgttcat
    13201 cgccattcac ctctcctcaa ccagcggcac catcacatca cgcgaccagg aaaccatcac
    13261 ctgttgtccg ggcgtgagcg tggcaggcag ttcttcgcct gtcatattgg cctgactcac
    13321 cagcagtttt tcaccgccgt tcaatttcag ttcaaaacga gttgccgcgc cctgatattg
    13381 caccgcctgg atcgtgccat tggcctgcag ttcaccaggg gtgttgaggc ggatatgttc
    13441 cggccgcagg gcgaagcttc ccgtcatgcc acaaagtttc tctgccatca gtccatcaaa
    13501 aacattcgat gtaccaacga acccggcaac aaacggcgtg cgcgggcgca tatagagatc
    13561 gcgcggggaa tcgacctgct caatgcgtcc attattgaaa accgccacac gatcggacat
    13621 cgataacgct tcgccctgat cgtgggtaac gcagataaaa gtgataccga gagactgttg
    13681 cagttttttc agctcaagct gcatctgctc acgcaatttg agatccagtg cgccgagcgg
    13741 ttcatccaac agcaatacgc gcggttcatt caccaatgct ctggcgatag caacccgctg
    13801 gcgctgacca ccagaaagtt gtgacggttt acgttgctgt acaaacccca acgccacttt
    13861 ctccagcgcc tcttgcgcca ttgcgtgccg ctgcttttta ttcacgcctt tgaccatcag
    13921 cccataggcg acattgtcaa gaatcgacat atgcggaaat agcgcgtagt cctgaaagac
    13981 agtattcacg tcccgctccc acggtggcag attgctggcg ggtttaccaa agatagagat
    14041 agccccgccg gaaagctgtt cgaagccagc aatcaggcgc aggcaggtgg ttttgccgga
    14101 gccggacggc cccagcatag agaagaactc accatctttt atcgcaatac tgacgccatc
    14161 tactgcgcgc acgtcaccgt acaaccgcga gacgttgtca aactccactg cgtacgtcat
    14221 aaagcacccc agcgaagtta gcgaccgccc ataatggcaa tgtaatcctg cgtccagcga
    14281 ctgtagggaa caaacttgcc cccttctgct ataggcgttt tccagaaggc tattttatcg
    14341 aaatagttaa aaccgtttgt ttcacaacct ttctcgccta ataacgaact ggctttacac
    14401 ccttccggca cgactggtaa cgagccaaac caggccgcca catcgccctg cacttttggc
    14461 gttaatgacc agttcatcca tttgtaggcg caaaccggat gtttcgcttc gctatgtagc
    14521 atggtggtgt cagcccaacc ggtaacgccc tccttcggga aaacggtagc gacaggctga
    14581 ccttcggctt tcagggcgtt ggtctgatag ggccaggcac tggaagcgac cacaccctcg
    14641 tttttgaaat cgctcatttg cacggtagtg tcatgccagt agcgatggat caaactatgt
    14701 tgatcacgca gcactttcag caccgcctgg tactgttctt cggtgagttg atacggatcg
    14761 ctgatgccca actgcggctg agtggcttta acgaacaacg cagcgtccgc aatgtagata
    14821 gggccatcat aagcctgaac gcggccttta ttgctcttgc cgtcctgcag attttgctca
    14881 acaaaaacca cttgccagct atccggcggc gtcgggaagg ttttagtgtt gtacatcagc
    14941 aggttcggcc cccattggta aggtgtgccg taaactttgc cgccaacgtt aaaccagtcg
    15001 cctttaacca cgcgcggatc aagcgttttc cagttgggaa tcaaggcggt attaatcggc
    15061 tgcacgcgtt tacccataat caaacgcagc gaggcatcgc cggatgccgt aaccagatcg
    15121 taaccccctt tggtcatcag actgaccatc tcatcggaag tcgcggcggt tttaacattc
    15181 accgcgcagc ctgtctcttt ttcgaattgc gttacccagt cgtattgttt atcagtttgt
    15241 ccgcgttcga tatatcccgg ccaggcgata atatccagtc gcccttccgg tttatctaaa
    15301 ttggtaggcg gttcggcggc gtgagcggtc attattgtca tgctgagcgc acacaggctg
    15361 ctgcgggcaa atgtcttgct cataaggtct tactcctgtc gtaattaatt gttcagccgc
    15421 cgtgccgtaa aaatatctcc ttgtttaacc atagacggcg gcttgcaggc acaccttcgc
    15481 ccctgagaaa atttaatcag gttgtgagct accgcgcatt atgccgtctg taaggttatc
    15541 ttctggagta tagacaagga gttaggaaat atgactgcta ttcgaaattc atattaccca
    15601 atctaagagg aattaaatta aacgctatta cagccgttct tgaataagtt tgcctaattg
    15661 ttttaccgcc tgttcttcac gttctcccca cagccacgcg gtattaaaac gaaaaaaacg
    15721 tgaccagttt tcaccggtag aaaacatttt acctggcgca atactgatat gatgcgtcag
    15781 tgccgccagg ctcaattcgc cggcatctaa cggctcgggg agctccagcc agagaaaata
    15841 accgctgtca ttatgatgaa ttttcacttc cgcaggcaga taacgcagca gtgcctgcca
    15901 ggcacgttgt ttacgttccg caagctggcg acgcaggcga cgaagatggg cgtcgtatcg
    15961 gcgcgtggaa aggtaatcca ccagcgcaag ctgcatcggt gagctggtgg aaagcgtact
    16021 catcagctgc aagcgttgaa ttttacgtgc atgttttccg gcggcgaccc aaccaatacg
    16081 aaaaccaggc accagacatt tcgaaaacga agagcaatgc aaaacgccat cgtggcgatc
    16141 ccacgctttc gcaggcagcg gtttttcccg tccaaaataa agttcgctgt aaacgtcatc
    16201 ttcaatcagc gttacgttgt actgattgag caacgccacc agttgtgctt ttttttgcgg
    16261 cgttaaggta aatccgagtg gattctggct attagtcatc agccagcacg ctttcaccgg
    16321 atactcctgc aacgccagtt ccagcgcctg aagatcgatc ccttctttaa catccgtcgc
    16381 caccgataac gccttcagcc gtagccgctc cagcgcctgc aacgcaccgt agaaacaagg
    16441 attctctact atcacccaat cgcccggttc agttaccgct tgcaaactga ggtttaatgc
    16501 ctctaacgcc ccggcagtga tgacaatttc atcaggagaa atggtgatcc cctgtaaggc
    16561 atagcgacgg gcaatagcct gacgcagttc tgcgtttcct ggcggcaagt tttcaatcac
    16621 gctcatcgcc gtggcggttt tgcttacctg cgccagcgag cggtttagtt gttggagggg
    16681 gaaaagtcgc gggtcgggaa aggccgaggc aaacggaacg accgacggat cgcgactggc
    16741 ctgcaacata tcaaaaatat aagtgttgat atcgactgct tcatctcgag tgactggaat
    16801 gactggcact ttcggcattt ttattgcctg tggcgcaacg taataacccg actgcggtcg
    16861 tgcgataata tatccctgac tttcgagcaa ctgataggca tggctgacag tcataaagct
    16921 catgccagaa agcgccacct ggtcacgcaa cgaaggtaaa cgatcgccgg gttgccagat
    16981 acccgacgca atctgctcgc gtaattgttc tgcaagctgc tggtattttt tcattatcga
    17041 atcgtaatta tgtgcagatg attcggcagt ctatatcagt tgttaaaaat gacaactttt
    17101 cgttaactgt aattaaacca tcaccagcga taactctttg ccaagcgcct ttgccgcgag
    17161 ctggactgcg tcgatttttg tcgcatgatg caagttaaat aggcgagtaa tctcttgttt
    17221 aggtttgcca attcgcctgg ctaactcttg ctgagtgatt tctgactgta aaaaagcatt
    17281 taacagcaat accttagagg cgacgctcaa aggtacttca ataaagtgat catgactatt
    17341 taatggcgaa ggtaaaggga taagctcgtt atcttcaaaa taaaaatcaa atgcggtcag
    17401 taaagcatct tttgccgctt ccatcgcttc agcgacagtt tcgccctgcg tcaacgcttc
    17461 agggatatcc acaaaagaaa ccatataacc gccttccggc gcgggtgtaa gagtgacggg
    17521 ataacgcata atttcgactg tctctcgcat gtcattaacc tttttaattg gcgattaact
    17581 caaaccgagt tgtttcagga ttgctttacg caatggttct ttaatctcat cgcaggggtg
    17641 acgcggcatg acactgcgcc tcccatgaaa cctgagtttc aaatggttgc tgccattcgc
    17701 tacatcgacg ccctgagatt cgagccaacg tctgaactcg ctttgtttca catcgcctcc
    17761 ctgcggctga gtatatcaac aaagtaaaca ttagtgttta cctccgtgtg taatttgctc
    17821 gaaaatagca ctaaatcgcc gcaacggcag aatgcaatgc aaaatcagga aggaagattc
    17881 cagaatttct ttgcgctaat ttgcaaaact gaataataac ctcaagaaac gatcggcatc
    17941 atagcggcaa aaggtaagaa aaaggtggca ggatgtttac gaaggcgtta tcggttgtct
    18001 tattaacgtg tgcgctgttc tcaggacaac tcatggcagg gcacaaagga catgaatttg
    18061 tgtgggtaaa gaatgtggat catcagctgc gtcatgaagc ggacagcgat gaattgcgtg
    18121 ctgtggcgga agagtcggcg gaaggtttgc gcgagcattt ttactggcaa aaatcgcgca
    18181 aaccagaagc gggacaacgt tgagtcgttt gatgccggat gcggcgtaac gccttacccg
    18241 gcaggcaata tcatgcagat tcaataagtt gtgagtaacc agaaaactgg cgttttaccc
    18301 ttttcgcttc ggcaatgttt tcagcagctc atccggactt acggatgcca ctacgcttcc
    18361 cggtaacggc attttcagta tgtgattttt gattttgcca atgacgtgca tctcgcatgg
    18421 gcggcaatca aactttagcg ttaatacttc atcgccgtta accagttgca ttggcgtcgc
    18481 cttccagctt ttgatattgc ctttcgcctg tttcgggcac agattaaagg caaagcgcag
    18541 acaatgcttg gtgatcatca ccgggacttc gcccttctct tcatgtgcct catacgccgc
    18601 gtcaatcagc tgcacaccat agcgatgata aaattcacgc gctttctggt tgtaaacgtt
    18661 cgcgaggaaa ctcagatgtg tttgcggata aaccggcgca ggatcagcaa ccggtttacg
    18721 gctgccgcgc tggtaactgg caagacgcgc agcatccagc atgtcggcag cttcacggcg
    18781 gaactggttt aacagactgt ttggtacaaa cagcgccccc ggcaaattaa tttgcacatc
    18841 gcgggcgtaa tagagggttt gccccagttt tgccagacca tccttcagat tgttcattgc
    18901 tttttctgcg ttattggctt cgtcgaactg cccatccagc gtatgcgtta tgctgacccc
    18961 ctcttcactg gtgagagtca gaatcaattg ttcctgccaa ccgcccagtt caatgtctac
    19021 cgccacccga cgttcgctag aggtttttgt cagtgcctgc tgccagttat gatcaagatt
    19081 acggtttagt gggtgatgcg ggcgaatttt gtgcaaatct gctggcattt cattgggcca
    19141 gacgcggtac tgattttctc cggttttctc gaccgtattg gcacgaaaac cgacgacttc
    19201 acgtttaatc aacacgttca ggccatcgcc atttgccagt ggctcggtaa cggcaacatc
    19261 gagatgatct ttcgccactt tcactacttc acctaccggc aggccgataa atttcggcga
    19321 atcgaacgcg ccaatatcgc ctttacgggc attcacaaaa taatctgtgc taccacggtg
    19381 gaaagtcttt tccgtcgatg gaacaaagaa atgttcagta cgacctgatg aagcgcgcgc
    19441 cagatcgcca cgttcttcaa taatggcatc aagcatctgg cgataatggg cggtgatatt
    19501 cttcacgtag ctcatatctt tgtaacgccc ttcaatcttg aaggaacgta cgccagcatc
    19561 aatcagcgcg ccgaggttgg cagtctgatc gttatctttc atcgacagca gatgtttttc
    19621 ataggaaacc acccgcccct gatcgtcttt caatgtgtat ggcaaacggc acgcttgcga
    19681 gcaatcgcca cggttggcgc tacgccctgt ttgcgcatga gaaatgtagc actgacccga
    19741 ataggccacg cacagtgcgc catgaataaa gaattcaatg gtcgcgtccg tggcctggtg
    19801 aatcgcgcgg atctgatcaa gattcagctc tcgcgccagc acaatctgcg tgaagccaac
    19861 atcagagagg aacttcgctt tttctactgt acgaatgtcg cactgcgtac tggcgtgcag
    19921 ttcaatcggc ggaatatcaa gttccagaat ccccatatcc tgaacaatca gcgcatcgac
    19981 accggtctgg tagaggtcag taatcagccg ttgcgcgggt tccagctcat catcatgcaa
    20041 aatggtgtta agcgtgacga aaatttttgc accataacga tgggcaaacg gcaccagctc
    20101 ggcaatatct ttcaggctat tactggcatt atgacgggca ccaaaaccag ggccgccgat
    20161 ataaacagca tcggcaccgt gcaaaatagc ttcgcgggca atggcggcat cgcgtgccgg
    20221 gcttaacagt tcaagtcgat gagaagatac ggtcacattc ttatccagta gctgaaaaat
    20281 ggcggctatt ttagccacaa gcgcgacgtt gcgaaatagt tttcccgctt aactgcgcgg
    20341 gtaatggatg agggaatgaa aatgtacggt ttgctcccca ccattacgat agatatgcgt
    20401 gacgtctgca gcaaatcgaa cgccttcccc acaattaagg gtctgccatt caccatcaac
    20461 gcacagatca agttgtccat caatgacgac gacatgttcg attaccccct tctcatgggg
    20521 cgtcgattcg cttattgcac cgggtgccat ctgaatcgag aagtgttcaa agcagagttg
    20581 tggatcataa ggaaacagcg aaatgatcac catcgcctgc tgttgtggat cataaactga
    20641 aggcgtagcg gactgtggcg gagaaataaa tgtcgaaaat ggcacgttca acccagtggc
    20701 aattttccat aatgtcgcga ccgtcgggct ggactcatta cgctcaatct gtcctaacat
    20761 tgctttagaa acgcccgtcg cttctgccag tcgcgaaaga ctccagccgc gctgctgacg
    20821 tagttgttta agtgtggtgg ataaaaagcg agcgagattt tccatgcgac tcctccggca
    20881 aaacggaagt ttatcacttg tgcgttataa cggacaaatg ctacggtgcc tgtacgctat
    20941 aacgcacgag gtgactatgc gtctgttttc tgttcctcca cccacgctac tggcggggtt
    21001 tctggcggta ttaattggct acgccagttc agcggcaata atctggcaag cagcgattgt
    21061 cgccggagcc accactgcac aaatctctgg ctggatgacg gcgctggggc tggcaatggg
    21121 cgtcagtacg ctgactctga cattatggta tcgcgtacct gttctcaccg catggtcaac
    21181 gcctggcgcg gctttgttgg tcaccggatt gcagggacta acacttaacg aagccatcgg
    21241 cgtttttatt gtcaccaacg cgttaatagt cctctgcggc ataacgggac tctttgctcg
    21301 tctgatgcgc attattccgc actcgcttgc ggcagcaatg cttgccggga ttttattacg
    21361 ctttggttta caggcgtttg ccagtctgga cggtcaattt acgttgtgtg gaagtatgtt
    21421 gctggtatgg ctggcaacca aggccgttgc gccgcgctat gcggtaattg ccgcgatgat
    21481 tattgggatc gtgatcgtta tcgcgcaagg tgacgttgtc acaactgatg ttgtctttaa
    21541 acccgttctc cccacttata ttacccctga tttttcgttt gctcacagcc tgagcgttgc
    21601 actccccctt tttctggtga cgatggcatc gcaaaacgca ccgggtatcg cagcaatgaa
    21661 agcagccggg tattcggctc ctgtttcgcc gttgattgtg tttactggat tgctggcact
    21721 ggttttttcc cctttcggcg tttattccgt cggtattgcg gcaatcaccg cggctatttg
    21781 ccaaagcccg gaagcgcatc cggataaaga tcaacgttgg ctggcagctg ccgttgcagg
    21841 cattttctat ttgctcgcag gtctgtttgg tagtgccatt accgggatga tggctgccct
    21901 gcccgtaagt tggatccaga tgctggcagg tctggcgctg ttgagtacca ttggcggcag
    21961 tttgtatcag gcgctgcata atgagcgtga gcgcgacgcg gcggtggtgg catttctggt
    22021 aacggcaagt ggattgacgc tggtcgggat tggttctgcg ttttggggat taattgccgg
    22081 aggcgtttgt tacgtggtgt tgaatttaat cgctgacaga aaccgataat aaaaagcgcc
    22141 atctaacgat ggcgcgtctg gaactacttt ttgttaacgt caaacatggt ggcatcggtc
    22201 gccatgtcat caataacttg tttaatattt tcgaaagcca ttggcgtact ttcgttattc
    22261 aggtctttac cttcgccctg acgaacgact ttgataaccg gtttattagt cgctgcatca
    22321 atcagctcac cttcaaaata caagcgagta tccatggtac ggtggcctgt agccatttgc
    22381 gtccccgcaa ccactaatgc aacaggaacc acttcataga attgcagccc ttctttgctg
    22441 gtatctacac cggtaatggc cccacggaaa atcagactac gcggccccgc ggtggtaacc
    22501 agtggtttac gctgcgctat cgcttctttc atttcggtgt tggtatagtt caaaatttta
    22561 tccagaactt tctgccctac ctgggtcgaa ggtttcggta ccggataata agtgattggg
    22621 ttccagacga tgctgtcata tttgctttga tcaaaactcg ggtctaccca acgtaaaacc
    22681 ggtttacctg tagccgaggt tgtttctttt aaatcagagt aattgtttaa aaaaccagaa
    22741 tatttatctg gctgggtgat tttagaggca caaccagaca gagccaacaa gccgcataaa
    22801 gctgcaactt tcgcaaat
//
LOCUS       DAZKIF010000080        22705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZKIF010000080 DAZKIF010000000
VERSION     DAZKIF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.080"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(653..1744)
                     /gene="ychF"
                     /locus_tag="ACHOTW_002986"
     CDS             complement(653..1744)
                     /gene="ychF"
                     /locus_tag="ACHOTW_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9845049.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1861..2445)
                     /gene="pth"
                     /locus_tag="ACHOTW_002987"
     CDS             complement(1861..2445)
                     /gene="pth"
                     /locus_tag="ACHOTW_002987"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9845050.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2723..3001
                     /gene="ychH"
                     /locus_tag="ACHOTW_002988"
     CDS             2723..3001
                     /gene="ychH"
                     /locus_tag="ACHOTW_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP9845051.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3056..4735)
                     /gene="dauA"
                     /locus_tag="ACHOTW_002989"
     CDS             complement(3056..4735)
                     /gene="dauA"
                     /locus_tag="ACHOTW_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP9845052.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4860..5807)
                     /gene="prs"
                     /locus_tag="ACHOTW_002990"
     CDS             complement(4860..5807)
                     /gene="prs"
                     /locus_tag="ACHOTW_002990"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9845053.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5958..6809)
                     /gene="ispE"
                     /locus_tag="ACHOTW_002991"
     CDS             complement(5958..6809)
                     /gene="ispE"
                     /locus_tag="ACHOTW_002991"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9845054.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6809..7432)
                     /gene="lolB"
                     /locus_tag="ACHOTW_002992"
     CDS             complement(6809..7432)
                     /gene="lolB"
                     /locus_tag="ACHOTW_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP9845055.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7646..8902
                     /gene="hemA"
                     /locus_tag="ACHOTW_002993"
     CDS             7646..8902
                     /gene="hemA"
                     /locus_tag="ACHOTW_002993"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP9845056.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8944..10026
                     /gene="prfA"
                     /locus_tag="ACHOTW_002994"
     CDS             8944..10026
                     /gene="prfA"
                     /locus_tag="ACHOTW_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9845057.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10026..10859
                     /gene="prmC"
                     /locus_tag="ACHOTW_002995"
     CDS             10026..10859
                     /gene="prmC"
                     /locus_tag="ACHOTW_002995"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9845058.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10856..11248
                     /gene="sirB2"
                     /locus_tag="ACHOTW_002996"
     CDS             10856..11248
                     /gene="sirB2"
                     /locus_tag="ACHOTW_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP9845059.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            11252..12061
                     /gene="sirB1"
                     /locus_tag="ACHOTW_002997"
     CDS             11252..12061
                     /gene="sirB1"
                     /locus_tag="ACHOTW_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP9845060.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12097..12951
                     /gene="kdsA"
                     /locus_tag="ACHOTW_002998"
     CDS             12097..12951
                     /gene="kdsA"
                     /locus_tag="ACHOTW_002998"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP9845061.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13099..13206)
                     /locus_tag="ACHOTW_002999"
     CDS             complement(13099..13206)
                     /locus_tag="ACHOTW_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9845062.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13635..13742)
                     /locus_tag="ACHOTW_003000"
     CDS             complement(13635..13742)
                     /locus_tag="ACHOTW_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9845063.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(14141..15241)
                     /gene="chaA"
                     /locus_tag="ACHOTW_003001"
     CDS             complement(14141..15241)
                     /gene="chaA"
                     /locus_tag="ACHOTW_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP9845064.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            15511..15741
                     /gene="chaB"
                     /locus_tag="ACHOTW_003002"
     CDS             15511..15741
                     /gene="chaB"
                     /locus_tag="ACHOTW_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP9845065.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15902..16597
                     /gene="chaC"
                     /locus_tag="ACHOTW_003003"
     CDS             15902..16597
                     /gene="chaC"
                     /locus_tag="ACHOTW_003003"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP9845066.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(16641..16994)
                     /gene="ychN"
                     /locus_tag="ACHOTW_003004"
     CDS             complement(16641..16994)
                     /gene="ychN"
                     /locus_tag="ACHOTW_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP9845067.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            17180..18574
                     /gene="ychO"
                     /locus_tag="ACHOTW_003005"
                     /gene_synonym="iatA"
     CDS             17180..18574
                     /gene="ychO"
                     /locus_tag="ACHOTW_003005"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP9845068.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(18575..19225)
                     /gene="narL"
                     /locus_tag="ACHOTW_003006"
     CDS             complement(18575..19225)
                     /gene="narL"
                     /locus_tag="ACHOTW_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP9845069.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(19218..21014)
                     /gene="narX"
                     /locus_tag="ACHOTW_003007"
     CDS             complement(19218..21014)
                     /gene="narX"
                     /locus_tag="ACHOTW_003007"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP9845070.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            21353..>22705
                     /gene="narK"
                     /locus_tag="ACHOTW_003008"
     CDS             21353..>22705
                     /gene="narK"
                     /locus_tag="ACHOTW_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP9845071.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WGPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     V"
BASE COUNT         5585 a         5653 c         5875 g         5592 t
ORIGIN      
        1 aaggtgagtt tagatcgagt tattccctaa gttgtcgtta cttattccaa ttcgttacta
       61 ttaatatgac aaaaacatta caccaaacag cggttaaata atttggtgaa tatttttggt
      121 aataatccta attagttatg ggattttcgc ttgatgtatc tgcataacaa aaacaatatg
      181 cgccattttt gcattactaa taagaaaaag caaaaaacat cgatattcat ttttggtcaa
      241 tgggttttct ggtctatcgt tgttattgtg ctactggtta tcattttcat cttgccagga
      301 tcgcacgtaa tcacgttatt cggggacttt atctgcggga tattaccctt caggtaagtc
      361 cttttctcgc cagcatattg gagcgtggtc aatattgatc ggctgtgaag ggggctgatg
      421 tgtagtggaa caaaaactga cgttatatca ctgctaatga gatatccgtc atctgaacac
      481 tgatgtcttt aattattctc gtggttcact gtaggcaata agcacaaaag tgtaggatgt
      541 tacaagaatg attaggactc ggtgaaatga aaaatccacg caattgcgtg gattttatat
      601 acttttgcgc tcttcatgag atttagcgaa acctcatgag acaataaatt aattagacgt
      661 tgaaaaggaa gttcatcaca tcgccatctt tcacgatgta atctttacct tctgcacgca
      721 ttttgcctgc ttctttcgcg ccttgttcac ctttgtaagt gatgaaatct tcaaacgaga
      781 tggtttgtgc acggataaag cctttttcaa aatcagtatg gattttgccc gctgcctgcg
      841 gcgcggttgc tccaaccgga atggtccatg cacgcacttc tttcacccca gcggtgaagt
      901 aagtttgcag gttcagcagc ttataaccgg cacggatcac acggttcaga cccggctctt
      961 ccagcccaag ctcctgcata aactcgtcac gttcttcgtc gtccagttcg gcaatgtctg
     1021 cttcaacagc agcacaaacc ggaaccacaa cagaaccttc tttcgccgcg atttcacgca
     1081 cctggtcaag atatgggttg ttttcaaaac cgtcttcgtt gacgttggcg atgtacattg
     1141 ttggtttcag cgtcaggaag ctcaggtaac gaatagccgc tttctcttca gcgcttaaat
     1201 ccagcgcgcg cagcatacct gcgttttcca gctggggcag gcatttttcc aggaccgcca
     1261 gctcagcttt cgcgtcttta tcgccacctt tggctttctt ctgtacgcga tgaatcgcac
     1321 gttcgcaggt gtcgaggtct gccagcgcca gttcggtgtt gataacttca atatcgtcag
     1381 ccgggttaac tttgccggaa acgtgaatga tgttgtcatt ttcaaagcag cgaacaacgt
     1441 gaccgatcgc ttcggtttca cggatgttgg tcaggaactg gttacccaga ccttcgcctt
     1501 tcgatgcgcc ttttaccaga ccggcgatat cgacaaattc catggtcgtg ggaagcgtac
     1561 gttgcggttt tacgatttca gccagttgat ccaggcgagg atcaggcatt ggtacgacgc
     1621 ctgtgttcgg ctcaatggtg cagaatggaa agttggccgc ttcaataccg gctttggtca
     1681 gcgcgttgaa cagggtagat ttcccgacgt tgggcaaacc gacgataccg catttgaatc
     1741 ccatgattta aatcacctta atatcttaat aatcaacctg ttattgctaa cagattgcag
     1801 aaatggaaat aactttgcct attatacacg gcactcggca aaaatgccgc agacaacgac
     1861 ttattgcgct ttaaaggcgt gcaatcggtt cgttgctttg gtcaagccat ctgtaaacca
     1921 catttcagta caacgcgccg cttcgtcgat ggcttcatca attaacttct gttcactaac
     1981 aggcggtttg cctaacacaa aaccaacaac tttgttttta tcgcccggat gaccgattcc
     2041 gatgcgtaaa cggtgaaagt tagggttatt acccaattta ctgatgatgt ctttcagtcc
     2101 attgtgacca ccatggccac cgcccaactt aaatttggcg acgccaggag gcagatccag
     2161 ttcgtcgtgg gccaccagaa tttcgtccgg attaatgcgg aaaaaactgg ccatcgccgc
     2221 aacggctttg ccgctgagat tcataaatgt agtcgggact aacaggcgga catcttcgcc
     2281 tccaagggtg actcgcgaag tataaccaaa gaatttagcc tcttcgcgca gcggagcgcg
     2341 caaacgctct gccagtaagt caacgaacca ggcaccagca ttatgtcgcg ttgcggcgta
     2401 ttcagcaccg gggttcgcca ggccgacaat caatttaatc gtcacgtttt tttgtcctga
     2461 gtgtgtacat aactggcgcg tagtttactg gttgcggccc cgcttgacaa aaaactgcgt
     2521 atcaaatgca gataacgtaa taattgcctg agtggactat tagaaagtca aggtgttcag
     2581 gcgtttattt gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg cccgcacata
     2641 acccacaggg tgttgtctat actttacaca taaggaagag gggtattccc tgttacaacc
     2701 cagaaagttc cggaggtgac atatgaaacg caaaaacgct tcgttactcg gtaacgtgct
     2761 catggggttg ggtctggtgg taatggtggt cggcgtgggg tattcaatcc tcaaccagtt
     2821 accacagttt aatatgcccc agtatttcgc acatggtgca gtgctaagta ttttcgtcgg
     2881 tgccattctc tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta
     2941 ctggtgggtt cgccactatg acaaacgttg ccgccgtagc gataatcgcc gtcatagcta
     3001 acaaataatg cagtttgctg accagtcagt ttgcgctgac tggtcaatct cgtacttata
     3061 aatccgccat cgccgcgcga cgattcggga agaacgcgag gcgtcccggg atcggttgaa
     3121 tgcctgcgcg agccatagtg cgcagtggct ggaattccac gttgcacacg cgcagttcac
     3181 atccttcggg cagacgcttc acaaaacgct ggaacgcatc aagaccacca gcatcaagta
     3241 ccggaacggc atcccacttc agaatcacaa tccgtttgcc ttcaagacgt gactccaggt
     3301 ccgtgaataa gccttcagca gcagcaaaaa acagcgggcc aataacgcgc agaaccagga
     3361 catcgtctgg aacatctacg actaccggtg ccaggcgagt catacgtgcg atacgacgca
     3421 taaacagcag cgatgccagc acgatcccca cgctgatggc aataaccata tcaaacagca
     3481 cggtcagcga catgcacagc agcatgacaa tgatgtcatc tttcggtgca tgacgcagca
     3541 agtcgaccac tttatgcgct tcactcatgt tccacgccac catcaacagc agggctgcca
     3601 tagcggaaag cggcagccag gagagcagcg gtgccagtac cagcagggca agaataacca
     3661 gaatagagtg gatcaccgcc gagatagggg aagttgcccc ggcacggacg ttagcggcag
     3721 aacgcgcgat ggcagcggta gcggtaatac caccaaagaa cggagcgatg atattcccca
     3781 gcccctgtcc aaccagttcg ctattcgcct tgtgcttcgt cccggtcata ccatccagca
     3841 ccacggcgca gagcagagat tcgattgcgc cgagcattgc cattgagaat gccgcaggca
     3901 gcagtgtgcg aatagaatcc caggttagcg tgaattctga attaggcaga tcccacggca
     3961 gcaccagttg cggtagcagt tgcggaatac cgttaccctg agaaccatcg gccaggacgt
     4021 agtggaattg cgaaccgatg gtagcaacat gtccgccgag caggttaaca atccccatca
     4081 ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa acgaatgccc agacgcggcc
     4141 agaaaacaag aatacctagc gtcacaatgc caatggcagc atcacccaca ttaatggtcg
     4201 gcagcgccat aaataatgcg ccgacttttt gtagataatg ttccgggaca tgggccattt
     4261 gcagaccgag aaaatcttta atctgcatgg taccgatggt gatcccgata cccgaggtga
     4321 aacctaaggt gacggaaacc ggaatatact caatcaggcg accaaagcgt gccagaccca
     4381 tcagaatcaa aaagatcccc gacagcaagg tcgcaaccag cagtcctgcc agcccaaact
     4441 gttgcgaaac gggatagaga attaccacaa atgccgcagt cggaccggaa acgctaaagc
     4501 gtgacccacc cgtcagagca atgacaatcc ccgcaacagc tgcggtatat aaaccgtact
     4561 ggggtgccac accactacca atagccaacg ccatcgccag cgggatagca ataatcccga
     4621 cggttatccc ggcaatcagg tcacgggtaa accgtgcggc agtatatttt tctttccagc
     4681 aagcgtcgat cagagcgcgg aaaggcatca catgtgagga aaatattttg ttcacaataa
     4741 tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag gcataggtca
     4801 tacaaatgga tattacagac aaaaaaaccc gccgcagcgg gtctttgagc cgggttcgat
     4861 tagtgttcga acatggcaga gatcgattct tcgttgctga tacgacgaat cgcttcggcc
     4921 agcatacctg acagggtcag agtacgcacg ttcggcagtg atttgatttc atcgctcagc
     4981 ggaatggtat cgcagacaac gacttcatca attacagagt tacgcaggtt gttcgccgcg
     5041 ttgccagaga agatcgggtg agtcgcgtac gcaaatacac gtttagcacc acgttctttc
     5101 agagcttcag cagctttaca cagcgtaccg ccagtgtcga tcatatcatc gaccagtacg
     5161 cagtcacgac ctgcaacgtc accgatgata tgcatcacct gggaaacgtt cgcacgcgga
     5221 cgacgtttgt cgatgattgc catatcggta tcgttcagca gcttagcgat agcgcgggca
     5281 cgcacaacgc cgccgatgtc cggagaaacc acaattgggt tatccagatt cagctgcagc
     5341 atgtcttcca gcaggatcgg gctaccaaat acgttatcaa ccggaacgtc gaagaaaccc
     5401 tgaatctgtt cagcgtgcag atccactgtc agcacacggt caacaccgac gctggagagg
     5461 aagtctgcaa ccactttcgc agtgattggt acacgagcgg aacggacgcg acggtcctgg
     5521 cgcgcatagc caaagtaggg gataacagcg gtgatacgac ctgcggaagc acgacgcagg
     5581 gcatcaacca taacgactaa ttccatcagg ttgtcgttag taggggcaca agtggactgg
     5641 atgatgaaaa tatcaccacc gcgtacattt tcattaattt gtacgctgac ttcgccatcg
     5701 ctaaagcgac ctacagcggc gtcgccgagt gaagtgtaca ggcggttggc aatacgttgt
     5761 gctagttccg gggtggcgtt accagcaaaa agcttcatat caggcacgag aagaacctca
     5821 ggcatgcgtc cattggtgga aagaatctgc cgaaaactgt gcgggccagg catgatcctt
     5881 tccaggcggt gtattaaaga gcgcgatgca acgtctggaa caaggtgacg ttgtcaccga
     5941 aactcagctt gcccggctta aagcatggct ctgtgcaatg gggaaagatt agcgcctttc
     6001 gccacaaagc cattgagcca ttccggggct tgctctagca cctggcgggc ttcagactct
     6061 gtatcaaatt cagcaaagac acaggcccct gtcccagtca ggcgcgacgg ggcgtattct
     6121 aacagccagg aaagcaccgc atcaacctcg cgaaaacgtt ttcttgcgat aacctcgcaa
     6181 tcattgctga attcacattt tagcaacgtt tctattgacc tttttggcgt attgcgcggg
     6241 agttcaggat ctttaaaaat caccggagtc ggaatactta caccagggtg cgccaccaga
     6301 taccacttct ctggcggatc caccggggtt agtatttcac caacgccttc ggcaaacgcg
     6361 gcatgccccc gaacaaagac aggaacatct gcgcccagcg tcagccccat ttccgccagc
     6421 tcatccatgc ttagcccgca ttgccagaga tgatttaatg ccaccaggac cgtcgcggca
     6481 ttggatgaac caccgccgag accgccgccc atcggcaaac gcttgtcaat gctgatattc
     6541 gcaccgcttc ccgtcggaag acgcccgctg tctgccgcag ttttcatcaa caaccgcgct
     6601 gcgcgaacga tcaggttatc ttcatgttcc acgccttcaa cgggcgttaa cagacggata
     6661 tccccatcgt cacgaagctc aatgctgatg gtgtcgccgt aatcaagaaa ctgaaacagc
     6721 gtttgcagcg tgtggtaacc atccgcacgc tgaccggtaa tgtataaaaa cagattaagt
     6781 tttgccggag agggccactg tgtccgcatt atttcactat ccagttatcc atttttaact
     6841 tgatgcgttg accaccgtcg gtgagttcca tattggctgg catcgcaggt tgcgttttgg
     6901 tgtcataacc accataaaca accttccagt ttttgccatt ctggctgtag gtaatttcgc
     6961 tcaggcggta ctggtcgtcc agtttgtagt cggttgcatc acccggtaaa cctaaaatcc
     7021 actggcgcaa gctgttgagc ggaattggca ttccagtcaa tttgccaatc atctcttcgg
     7081 cgtcatcggc ggtataacgc tgacctttat tgtcgactaa ctgcacgtta cccggttgag
     7141 cattcagctc cagttccgtg ctgcccaatg ggttagtgag cagcagacgg tagcgatcct
     7201 ggccggtttg ctgccagaaa aagcgggcgt acactttttg ttggtcagaa atataagcga
     7261 acgcgccgcg agtctgatac tgattaagat tgcgcacgtc ttgctgatgc tgacgccatt
     7321 gtggcgaatc cgggcttttg ccaggacctt tgggcgtggt aacggaacag gcagtgagca
     7381 caagagcagc cagcggtagc aggcggataa gacgaaaatc gggcaggggc atagtgatga
     7441 caagtccttg agatacgttg cagttataac ccttaatgct agcgtcacca ttagtcatcg
     7501 tctatgttca agttgtctta attgtagaaa tctaacgggt tcgggcaatt actccaaaag
     7561 ggggcgctcc cttttattga tctcgcgcat acggtatgat gcgccgagac taaccttatc
     7621 aacgttggta ttatttcccg cagacatgac ccttttagcg ctcggtatta accataaaac
     7681 ggcacctgta tcgctgcgag aacgtgtatc gttttcgccg gacaaacttg atcaggcgct
     7741 ggacagtctg ctcgcacagc cgatggtgca gggcggggtg gtgctgtcga cgtgtaaccg
     7801 cactgagctt tatcttagcg tcgaagagca ggacaacttg caagaggcgt taatccgctg
     7861 gctttgcgat tatcacaatc ttaatgaaga agatctgcgt aaaagcctct actggcatca
     7921 ggataacgac gcggttagcc atttaatgcg tgttgccagc ggcctggatt cactggttct
     7981 tggggagccg cagatcctcg gtcaggttaa aaaagcgttt gccgattcgc aaaaaggtca
     8041 tatgaaggcc agcgaactgg aacgcatgtt ccagaaatct ttctctgtag cgaaacgcgt
     8101 acgcactgaa acagatatcg gtgccagcgc tgtgtctgtc gcttttgcgg cttgtacgct
     8161 ggcgcggcag atctttgaat cgctctctac ggtcacagtg ttgctggtag gcgcgggcga
     8221 aaccatcgag ctggtagcgc gtcatctgcg cgaacataaa gtacagaaga tgattatcgc
     8281 caaccgcact cgcgaacgtg cccaaattct ggcagatgaa gtcggcgcgg aagtgattgc
     8341 cctgagtgat atcgacgaac gtctgcgcga agccgatatc atcatcagtt ccaccgccag
     8401 cccgttaccg attatcggga aaggcatggt ggagcgcgca ttaaaaagcc gtcgcaacca
     8461 accaatgctg ttggtggata ttgccgttcc gcgcgatgtt gagccggaag ttggcaaact
     8521 ggcgaatgct tatctttata gcgttgatga tctgcaaagc atcatttcgc acaacctggc
     8581 gcagcgtaaa gccgcagcgg ttgaggcgga aactattgtc gctcaggaaa ccagcgaatt
     8641 tatggcgtgg ctgcgagcac aaagcgccag cgaaaccatt cgcgagtatc gcagccaggc
     8701 agagcaagtt cgcgatgagt taaccgccaa agcgttagcg gcccttgagc agggcggcga
     8761 cgcgcaagcc attatgcagg atctggcatg gaaactgact aaccgcttga tccatgcgcc
     8821 aacgaaatca cttcaacagg ccgcccgtga cggggataac gaacgcctga atattctgcg
     8881 cgacagcctc gggctggagt agcagtacat cattttcttt ttttacaggg tgcatttacg
     8941 cctatgaagc cttctatcgt tgccaaactg gaagccctgc atgaacgcca tgaagaagtt
     9001 caggcgttgc tgggtgacgc gcaaactatc gccgaccagg aacgttttcg cgcattatca
     9061 cgcgaatatg cgcagttaag tgatgtttcg cgctgtttta ccgactggca acaggttcag
     9121 gaagatatcg aaaccgcaca gatgatgctc gatgatcctg aaatgcgtga gatggcgcag
     9181 gatgaactgc gcgaagctaa agaaaaaagc gagcaactgg aacagcaatt acaggttctg
     9241 ttactgccaa aagatcctga tgacgaacgt aacgccttcc tcgaagtccg agccggaacc
     9301 ggcggcgacg aagcagcgct gttcgcaggc gatctgttcc gtatgtacag ccgttacgcc
     9361 gaagcccgcc gctggcgggt agaaatcatg agcgccagcg agggtgaaca tggtggttat
     9421 aaagagatca tcgccaaaat tagcggtgat ggtgtgtatg gtcgtctgaa atttgaatct
     9481 ggcggtcatc gcgtgcagcg tgttcctgct acggaatcgc agggtcgtat tcatacttct
     9541 gcttgtaccg ttgcggtaat gccagaactg cctgacgcag aactgccgga catcaaccca
     9601 gcagatttac gcattgatac tttccgctcg tcaggggcgg gtggtcagca cgttaacacc
     9661 accgattcgg caattcgtat tactcacttg ccgaccggga ttgttgttga atgtcaggac
     9721 gaacgttcac aacataaaaa caaagctaaa gcactttctg tactcggtgc tcgcatccac
     9781 gctgctgaaa tggcaaaacg gcaacaggcc gaagcgtcta cccgtcgtaa cctgctgggg
     9841 agtggcgatc gcagcgaccg taaccgtact tacaacttcc cgcaggggcg cgttaccgat
     9901 caccgcatca acctgacgct ctaccgcctg gatgaagtga tggaaggtaa gctggatatg
     9961 ctgattgaac cgattatcca ggaacatcag gccgaccaac tggcggcgtt gtccgagcag
    10021 gaataatgga atatcaacac tggttacgtg aagcaataag ccaacttcag gcgagcgaaa
    10081 gcccgcggcg tgatgctgaa atcctgctgg agcatgttac cggcagaggg cgtactttta
    10141 ttctcgcctt tggtgaaacg cagctgactg acgaacaatg tcagcaactt gatgcgctac
    10201 tgacacgtcg tcgcgatggt gaacccattg ctcatttaac cggggtgcga gaattctggt
    10261 cgttgccgtt atttgtttcg ccagcgacct taattccgcg cccggatacg gagtgtctgg
    10321 tggagcaggc actggcgcgg ttgcctgaac aaccttgccg tattctcgat ctcgggacgg
    10381 gtaccggggc gattgcgctt gcgctggctc gcgagcgccc ggactgcgaa attaccgctg
    10441 tagatcgtat gcccgatgct gtcgccctgg cacaacgtaa tgcccagcat ctggcgatca
    10501 aaaatatcca cattctgcaa agcgactggt ttagcgcgct agccgggcag cagtttgcga
    10561 tgattgtcag caatccgccg tatattgacg agcaggaccc tcatcttcaa caaggcgatg
    10621 tccgctttga gccgctcact gcgctggttg cggcagacag tggaatggca gacatcgtgc
    10681 atatcatcga acagtcgcgt aacgcgctgg tatccggcgg ctttctgctt ctggaacatg
    10741 gctggcagca gggcgaagcg gtgcgacagg catttatcct cgcggggtat catgacgtcg
    10801 aaacctgccg tgactatggt gataacgagc gcgtaacgct cggccgctat tatcaatgac
    10861 aagtttttct acactgctta gtgttcatct tattagtatc gcgctttctg ttgggctatt
    10921 aaccttacgt ttctggctac gttatcagaa gcatccacag gcatttgctc gctggacgcg
    10981 cattgtgccg ccggttgtcg atacggtgtt attgttaagc ggcattgcgt tgatggctaa
    11041 agcgcacatc ctgccatttt ccgggcaggc acagtggctg actgaaaagc tgtttggagt
    11101 tatcatttat atcgttttgg gttttattgc actcgattat cgtcgtatgc acagtcagca
    11161 ggcgcgcatt attgccttcc cgctgacgtt ggtggtgctg tacatcatca ttaaactcgc
    11221 caccacaaaa gtaccgttac tggggtaagt catgagatcg ttagctgatt tcgaatttaa
    11281 taaagcgcca ttgtgcgaag gcatgatcct ggcttgcgaa gcaatccgcc gcgattttcc
    11341 ctcgcaagat gtttacgacg aactggagcg tctcgttagt ctggcgaagg aagaaatcag
    11401 ccagcttctg cctttagaag agcagctgga aaaactgatc gcgctgtttt acggcgactg
    11461 gggatttaaa gcctcacgcg gcgtttatcg tctttccgat gcattatggc tggaccaggt
    11521 gttaaagaat cgacagggca gtgcggtatc attaggtgcg gttttattat gggtcgcgaa
    11581 tcgtctcgat ttgccgctgc tgccggtgat tttccctacg cagctgatat tgcgcattga
    11641 atgtccggat ggcgaaattt ggctgattaa tccttttaac ggtgaatcgt taagcgaaca
    11701 tatgctggac gtatggttaa agggaaatat cagcccgtcg gcggaactgt tttatgaaga
    11761 ccttgatgaa gctgataaca ttgaggtaat ccgcaaattg ctggatacac tcaaagcctc
    11821 gttgatggaa gaaaatcaga tggagctggc gttacgcacc agcgaagctt tattacaatt
    11881 caacccggaa gatccctatg aaattcgcga tcgcgggttg atttatgcgc aactggattg
    11941 cgaacacgtt gcgttgaacg atttaagtta tttcgttgaa cagtgtccgg aagacccgat
    12001 cagcgaaatg atccgtgcgc aaataaataa catcgcgcat aaacatattg tgctgcatta
    12061 attaatcgac attttactca aggttaaggc gatcctatga aacaaaaagt ggttagcatt
    12121 ggcgacatca acgtagcaaa tgacctgccg ttcgtactgt ttggcggtat gaacgtgctg
    12181 gaatctcgcg atctggcgat gcgcatttgc gagcactacg taaccgtgac ccagaaactg
    12241 ggtatccctt acgtgttcaa agcctctttt gacaaagcca accgctcctc catccactct
    12301 tatcgtggac cgggcctgga agaagggatg aaaatcttcc aggagttgaa gcagactttt
    12361 ggcgtgaaaa ttatcaccga cgttcacgaa ccaagtcagg cacagcccgt tgctgatgtc
    12421 gtggatgtga ttcagttgcc ggcgtttctt gctcgccaga ctgacctggt tgaagccatg
    12481 gcgaaaaccg gtgcggtaat taacgtcaag aaaccacagt ttgtcagccc gggacagatg
    12541 ggtaatatcg ttgataaatt caaagaaggc ggcaacgaaa aagtgattct ttgcgatcgt
    12601 ggtgctaact tcggctatga caacctggtt gtcgatatgc tgggcttcag cattatgaag
    12661 aaagtgtctg gtaactcgcc ggtgattttc gacgtgaccc acgcattgca atgccgcgat
    12721 ccttttggcg cagcttccgg cggtcgtcgt gctcaggtgg ctgagctggc acgagccggt
    12781 atggcggtag gtctggcggg gctgtttatt gaagcgcatc cggatccgga acatgcgaaa
    12841 tgtgatggtc catccgcgct gccgctggct aaactggaac cgttcctcag gcagatgaaa
    12901 gcgattgatg atctggtgaa aggtttcgaa gaactggata ccagcaagta atcttttttg
    12961 cttgaaaaat aaagtcttag cgttctgcgt taagactttt ttcatgggtg ccggatacaa
    13021 aaaaggccgc aggctgttac cctgcggccg gtttcgggcg catattgcca tcacggcagc
    13081 ctgacgcccg ttttcgcctt acttccggtt acgccaccag ccgacaatcg ctgcggtaat
    13141 aattcccgcc aggatcggtg ctgccaggtc gtgccagaaa atcatggcaa actgcgcgag
    13201 cgtcatatag ccgccttgtt gtaatgacaa cattttgcgg ctattcttga attgttgatg
    13261 gttcaagatt agcccccgtt ctgttgtcag gttttacctc tcaacgtgcg ggggttttct
    13321 ctttccagca accaatgcca ccagggataa agcccccgca acattgcgcc tcaccggata
    13381 acgccggctt ggtgtggata ctacgactca attcatcttc acttcatccc tgaaatgttt
    13441 gcaatgaaga gtgcattccg gtttttcaac agctgttaca gtcgtttcat gagtgctctg
    13501 gatgatgctt ccagctcggg ttgccaatat ttacttgtgg aagtgataaa gacaaaaatg
    13561 gccgcaggct gttacccctg cggccggttt cgggcgcata ttgccatcac ggcagcctga
    13621 cgcccgtttt caccttactt ccggttacgc caccagccga caatcgctgc ggtaataatt
    13681 cccgccagga tcggtgccgc caggtcgtgc cagaaagtca tggcaaactg cgcgagcgtc
    13741 atatagccgc cttgttgtaa tgacaacatt ttgcggctat tcttgaagtg tctggtttca
    13801 agattagccc tgttgtcagg ttttacctct caacgtgcgg gggttttctc tttccagcaa
    13861 ccaatgccac cagggataaa acccccgcaa cattgcgcct caccggataa cgccggcttg
    13921 gtgtggatac tacgtctcaa ttcatcttca cttcatccct gaaatgtttg caataaagag
    13981 tacattccgg cttttcaaca gctgttgcag tggtttcgtg caggttgtgg tacaggctcg
    14041 caattctgat taacgacctg tccaggtagt atgaagaaat acaataagca ggaatttata
    14101 ttatctcccc cggtttccgt catcggagga gatataactg tcaggcaaat atcgtcatca
    14161 aataggcggc aaacagtgcc agatgcgctg cgccattgag cacgttagta cgtccggtgg
    14221 agaaggagat atggcacagc actaaagagg ccaccatcac caccatttct ggcgcaccaa
    14281 gtgcaaactg caattcgtta cccgtcataa aggcaattag cgtgacgaca ggtacggtaa
    14341 gcgaaattgt tgctaacact gaaccaaaga acagattcat cgcgcgctgg acctggttgt
    14401 tcaacactgc ttttaatgca cctaaacctt ccggcgacag aatcaacagt gccaccagga
    14461 agccagtaaa ggcgacaggg gcattcatgc tgtcgagcaa agtctccagc gggctggcgt
    14521 tcattttggt gactgcaata acggcaatca ggtggataat caaccagata gcatgccaca
    14581 ggctgctatg ggcagacggt ttaccgtggt gcgggtcgtc gtcatcactg tcatcttcgt
    14641 gctcgtagac aaacaaactt tgatgcgttt tggtctggat cagcaaaaat acaccataca
    14701 ttgccgcaga aattaatgct accagtaacg cctgaccggt tgaaaaattc gccgcaggca
    14761 gagccattgg aaataccagt acgattatcg ccagggggaa cagcgcaatt aaatactgct
    14821 taataccaaa cagattcata tattgggtgg caaacttacg accgcccaac aataatgaaa
    14881 agccgaccag cccaccggta acaatcataa tgatcgaata gagcgtatca cgcattagcg
    14941 ttggcgcggc gtcgccggtt gccattaaag ctgaaatcaa actaacttca agaataacca
    15001 ctgaaaggct aagaataagc gaaccgtaag gttctcccag gcgatgggct aatacgtccg
    15061 catgacggac aacactaaag gcgctactta aaataccaat aagcgcaaga agattgatgg
    15121 caatgaccac tggtagtgtc tggctgcttc cccacaggaa cagcactacc agcgccagaa
    15181 ccgggaaaat aagcgaagtc tccttgtggc gggtttttac cgcctcttga gcatttgaca
    15241 ttatggttat ccctttgcag atgaatttat cgaaaatgta aaaaataggt aggaaaaata
    15301 acagaaagtg tctggatatc ggtaacattt tacgaatttt taccccgttg tcgatttttt
    15361 actcattggg gcataaaaat aagtattacg tttaggcaat gtttgtttta gcgtttctta
    15421 agaacagtct gacatgaaaa ttcttatgtt tttgcaagag tagatattgt tgaccacact
    15481 taatgttcaa ctttgtaaaa ggagtcaacg atgccgtata aaacgaaaag cgacctgccg
    15541 gaaagcgtaa agcacgttct accgtctcat gcccaggata tctataaaga agcgttcaac
    15601 agcgcatggg atcaatataa agataaagaa gatcggcgtg atgacgccag tcgcgaagaa
    15661 accgcgcata aagtggcctg ggctgctgtg aagcatgaat atgccaaagg ggatgatgat
    15721 aaatggcata aaaaatcgta aaaccggtag cttagttaaa gctattcgtg cggtgttgcc
    15781 ttgcaagtgg tccgcggatt gcatattgtc ccgttagtgg tttcaaaatg agcagtaaaa
    15841 atgtccggaa gacaccaaaa agttgtcgca gggaagcatg cagtggcgga ggtgtaagaa
    15901 ggtgataacg cgtgatttct tgatgaatgc cgattgtaaa acggcatttg gtgccattga
    15961 agaatcactc ttatggtcag cagaacaacg ggcggcttcg ctggcggcga cgctggcttg
    16021 tcgacctgat gagagaccgg tgtggatctt cggttatgga tcgttgatgt ggaatccggc
    16081 actggagttt accgagtcgt gcaccggtac actggttgga tggcatcgcg cgttttgcct
    16141 gcgcctgacc gccgggcgcg ggactgcgca ccagccggga cggatgcttg cactaaaaga
    16201 gggcggacgt accacaggcg tcgcctatcg actgccagaa gagacgctgg agcaggaact
    16261 aaccctgttg tggaagcgcg agatgattac cggctgttat ctgccaacct ggtgtcagct
    16321 tgatcttgat gatggacgca cagtaaacgc cattgtgttt attatggacc cgcgacatcc
    16381 agaatatgaa tctgacactc gcgctcaggt catcgcgccg ttgattgcgg cggcgagcgg
    16441 tccgctggga accaacgcac aatacctgtt ttcactggaa caggagctta tcaaactggg
    16501 aatgcaggac gatgggctga atgatttgct ggtatcggta aaaaaactgc tggcggagaa
    16561 ttatccggat ggtgtgttgc ggccgggatt cgcctgagta aacttccctc atcgtggggc
    16621 gtcagacgcc ccctcaaaca ttaaaatgtg agcactttat cggctgacag cgtccattgc
    16681 gccagttcca caagagtacc gatttccacc ccatcaatca gaggaagtgt actaatcccg
    16741 cgcccgtcgg tacaggtttt gcacaatttc accggtacat tctgagcggt aaggatctcc
    16801 agcatttgct gtatgttgta gccttcccct ggtttttgcc cgcgcaaccc ggctgtgacc
    16861 gcatcagaca tcaggaacag acgcagatcc agattgctct cctgctctcg taacgcaatg
    16921 gccagccgca agctgttaaa caaggattcg ctcccgtaag gtgcgccatt ggcaacgatc
    16981 acgatttttt gcattattta ctcctgtatt cagggaatta gacactcatc ttctatctta
    17041 ctgcttctgc agcgtctgac caatcggtca catttttaag gattttcctg aaagcgcgag
    17101 aaaatacgac aaaagttgcc agtaatcgtt attctttaag gctatgtttt ttcatttttt
    17161 accggaagtt accgacgttt tgagccgttt cgttcctcgc attattccgt tttatttact
    17221 cttgctggcg gcaggcggta cagctaacgc acaatctacc ttcgagcaaa aagcggcaaa
    17281 tccctttgat aataacaatg atggtctgcc ggatttaggc atggcgcctg aaaatcatga
    17341 tggggaaaaa cactttgctg aaattgtgaa agatttcggc gaaaccagta tgaatgataa
    17401 cgggctggat actggcgagc aggcaaaagc tttcgcattg ggaaaagtcc gcgacgcgct
    17461 tagtcaacag gttaatcagc acgtagagtc gtggctatca ccgtggggaa atgccagtgt
    17521 tgacgtcaaa gtggataacg aaggtcattt taccggcagt cgtggaagct ggtttgtgcc
    17581 gttacaagat aatgatcgtt atctcacctg gagccagctt ggtcttactc agcaggatga
    17641 tgggctggtg agcaatgtgg gcgttgggca acgctgggcg cgcggcaact ggctggtggg
    17701 ttataacact ttttatgaca acttgctgga tgaaaatctt cagcgagcgg gctttggtgc
    17761 cgaagcgtgg ggcgaatatt tgcgactatc ggcaaacttt tatcagccgt ttgctgcatg
    17821 gcatgaacag acagccacgc aggaacaacg gatggcgcgc gggtacgacc tgacagcccg
    17881 gatgcgcatg ccgttctatc aacacctcaa taccagtgtc agcgtagaac agtattttgg
    17941 tgatcgtgtt gatttgttta actctggtac gggttatcac aatcccgtcg cgttgagtct
    18001 gggattaaat tacacccctg tgccattagt cactgtgacg gcccagcata aacagggtga
    18061 aagtggcgag aatcaaaata acctcgggct gaatcttaat taccgctttg gtgtaccgct
    18121 caaaaaacaa ctttctgcgg gcgaggttgc cgaaagtcag tcgttacgtg gtagtcgcta
    18181 tgataatccg cagcgaaata atctaccgac tcttgagtac cgacagcgaa aaacgttaac
    18241 ggtgtttctt gcgacaccgc cgtgggatct aaaacctggc gaaacagtgc cgctgaaatt
    18301 acaaatccgc agtcgttacg gtattcggca actgatttgg cagggcgata cgcagatatt
    18361 aagtttgacg ccgggcgcac aagccaacag cgcggagggc tggacgctga tcatgcctga
    18421 ctggcagaac ggggaagggg cgagcaatca ctggcgattg tcggtggtgg tggaagataa
    18481 ccaggggcag cgtgtctcct ccaatgagat cacgctaacg cttgtcgaac cgttcgacgc
    18541 attgtcaaac gacgaactgc gctgggaacc gtaatcagaa aatgcgctcc tgatgcaccc
    18601 ataccgctgc ttccacgcga gacttgagct tcattttctt cagcatgtgc ttgacgtgca
    18661 cttttactgt gctttcggtg atgtccaggc ggcgggcaat catcttgttc ggcaaaccct
    18721 gggcaatcag cttgagaata tcgcgctcgc gtggggttaa ctggttaaca tcgcgctcag
    18781 tcgtggcacg gttagcgcgc aagctggcgg ccagaacagg cgttaatgct tcgcttaata
    18841 ccatttcgcc agcagctgcc tgatgcaatg ctttcagcag atcttccggt tccatatctt
    18901 ttaacagata gccatccgcg ccgcgtttca gtgcggtgac cacatcttct tcatggttag
    18961 agacgctgaa taccacaatg cgccctgaga gggacttttc gcgcagttta tccagcgttt
    19021 ccagaccgtt catgccgggc atattgagat ctaacaggat cagatcggga tcaagagatt
    19081 ccgccagttc aataccctgt tcgccattac tcgcttcgcc aaccacggtg atatctggtg
    19141 ccatactgat aagctgtttt acgccagttc gcagcatcgg gtgatcgtca atcagcagga
    19201 tagtagccgg ttcctgatta ctcatgggta tctccttgga cgtctgtgaa agttttttcg
    19261 ggaataaagg tgaccaccac ttcggtgcca cctgattcac gacggcggac gcggcaatcg
    19321 cctcgtaaac tttgcgcacg gtcgcgcatt attatcatgc cgtagtgatt gctgcggatg
    19381 gcattttcag gcacgccgca gccgttatcc tggacggtca gtttgacctg attatcgttt
    19441 tgcgccaccg tcaccacgac ctcactcgct tgcgaatgtt tgagggcgtt acttaatgcc
    19501 tcacgggcaa tttgcaacaa gtggattgcc tgatgcgaag gcaccagacg aggcggcaat
    19561 tgataatcca gcttcaccgg gaagccaaat ttggcgctgt actcttcgca actcgcctcc
    19621 agcgccggac gtaatccagg ctcggtgagc tgcaagcgga atgtggtgag caattcacgc
    19681 aactgcgccc aggatgcatt cagttcgtta cggatctgac ttaacagttc gcggctgctt
    19741 tctggcagcg catcgccctg catctgtaaa caactcacct gcatcttcat gcaagagaga
    19801 gattgggcaa tagaatcatg cagttcgcgc gcaatggtgg cacgctcttc catcacgatc
    19861 aactgttgct gacgttcctg atgccgatcc agcgccagcg tggcggtgag ttgttcaacc
    19921 agggtatcca ccagttgttg ttgatcatgg ctaagatgac gcccctgcgg tagggtcgcc
    19981 agcaaaatac cgtactgcgt atgagagtca gccagccgcc acttcagggt cgtgccgcga
    20041 tcaccaacgg gtaatacgcc gcgcgggcag agctggcagc ctttatcatc acaagtcata
    20101 tctggctggc aggtaaactc ctgatgattc tcttcatcat cagtgtcata cacccgcagt
    20161 tcgatatcac gtagcagggt taaattctgt aagccgttga gtacaggtga caggcgttca
    20221 cacagcgggg cgcgggaatg caaacggcgg ttagcctgcc ataaaaaaga gaggatctga
    20281 tttttatgct ccagcccggc ggttttctcc tgaacccgct gctcaagtac ggcataactt
    20341 tcggccagtt ctgcagacat attgttcaac gcagttccaa gcatcgccat ttcgttgcgc
    20401 ccgctgatgt ttgcgcgttg ggtaaaatcg cgatggctga cggcactcgc cattgccagc
    20461 agttgccgcc acggttgtag cagtcgcgcc cgcaaccaga taatagtgaa caccagtaaa
    20521 agtgccataa ataccgccat tacccgatgg accagtacca ctgtctcaat gcgcatttcc
    20581 gtggtgcggt caaaaccaga taccagctga tcaagcccgg caacaaactg gctgacatcc
    20641 gctgacaccg tttcgcggtt ttgtgcacgc atcagtgcag ggatgagttc attacgccag
    20701 taatcttgta aaccctgtaa ttgcgccagt tgtccgtctc gttctgctgc tcgagtcaac
    20761 tcggcgctaa atgccgtttg ttccatctct ttaattaagg gcttgtcttt ctcgcttaat
    20821 ggcactgctg ccaacagacg gtaactttgc atgcgcagcg atcccgcttt gttgatcgca
    20881 tgggcgctgc cctgaacgcc ttgcaccagc cagccagaaa ccgccatccc tgccagtcca
    20941 atagcagtag aaagcaacac aataagcgca acctgattaa ccagggtgag cggagagaga
    21001 caacgtttaa gcatgtaaac ctcttccttc aggctttaaa tgagcaataa ccttaatgaa
    21061 tgtgacgata cattctggaa tggcagtatt ctcggctatt ggctgaagta tacccatacc
    21121 cggaaagagt tactccttat ttgccgtgtg gttagtagct ttacatcggt aagggtaggg
    21181 attttacagc accgtgaaaa atctcataat ttttatgaaa tcactgtact cactatgggt
    21241 aatgataaat atcaatgata gataaagtta tcttatcgtt tgatttacat caaattgcct
    21301 ttagctacag acactaaggt ggcagacatc gaaacgagta tcagaggtgt ctatgagtca
    21361 ctcatccgcc cccgaaaggg ctactggagc tgtcattaca gattggcgac cggaagatcc
    21421 tgcgttctgg caacaacgcg gtcaacgtat tgccagccgc aacctgtgga tttccgttcc
    21481 ctgtctgctg ctggcgtttt gcgtatggat gttgttcagc gctgttgcgg tgaacttacc
    21541 gaaagtcggt tttaatttta cgaccgatca gctatttatg ttgactgcgt tgccttcggt
    21601 ttctggcgcg ttattacgtg ttccatactc ctttatggtt cctatcttcg gtggtcgtcg
    21661 ctggacggcg ttcagcaccg gtattctgat tattccttgt gtctggctgg gttttgccgt
    21721 gcaggatacc tccacgcctt atagcgtctt catcatcatc tctctgctgt gcggctttgc
    21781 tggcgcgaac ttcgcatcca gtatggcaaa catcagcttc ttctttccga aacagaagca
    21841 gggtggcgcg ctgggcctga atggtggtct gggcaacatg ggcgttagcg ttatgcagtt
    21901 ggttgctccg ctggtggtat cactgtcgat tttcgcagta tttggtagcc agggcgtcaa
    21961 acagccggat gggactgagc tgtatctggc gaatgcgtcc tggatatggg ggccgttcct
    22021 tgccatcttc accattgcgg cgtggtttgg catgaacgat cttgctacct cgaaagcctc
    22081 catcaaggag cagttgccgg tactcaaacg gggtcatctg tggattatga gcctgctgta
    22141 tctggcaacc ttcggttcct tcatcggctt ctccgcgggc tttgcgatgc tgtcaaaaac
    22201 gcagttcccg gatgttcaga ttctgcaata cgctttcttc gggccgttta ttggtgcgct
    22261 ggcgcgttct gcaggtggtg cattatctga ccgtctgggc ggaactcgtg tcacgctggt
    22321 gaactttatt ctgatggcga ttttcagcgg cctgctgttc ctgaccttac cgactgacgg
    22381 gcagggcgga agcttcatgg cgttcttcgc ggtcttcctg gcgctgttcc tgacagctgg
    22441 gctgggtagt ggttccactt tccagatgat ttccgtgatc ttccgtaaac tgacaatgga
    22501 tcgcgtgaaa gcagaagggg gttctgacga acgtgcgatg cgtgaagcgg caaccgacac
    22561 ggcggcggcg ttgggtttca tctctgcgat tggcgcgatt ggtggcttct ttatcccgaa
    22621 agcgttcggt agctcgctgg cattaacggg ttcgccagtc ggcgcaatga aagtattttt
    22681 gattttctat atcgcctgcg tagtg
//
LOCUS       DAZKIF010000081        22665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZKIF010000081 DAZKIF010000000
VERSION     DAZKIF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.081"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            712..4524
                     /locus_tag="ACHOTW_003009"
     CDS             712..4524
                     /locus_tag="ACHOTW_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFP9845072.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINARGSIENRDGNNFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRLIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNSL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4613..6232
                     /locus_tag="ACHOTW_003010"
     CDS             4613..6232
                     /locus_tag="ACHOTW_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP9845073.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTSVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGISEYLSAKATHTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQIENGYYNTAFFAQYAPRELTLQDKITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGSAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            6248..6616
                     /gene="acpS"
                     /locus_tag="ACHOTW_003011"
     CDS             6248..6616
                     /gene="acpS"
                     /locus_tag="ACHOTW_003011"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9845074.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTSHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            6630..7391
                     /locus_tag="ACHOTW_003012"
     CDS             6630..7391
                     /locus_tag="ACHOTW_003012"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9845075.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIERQAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            7395..7943
                     /locus_tag="ACHOTW_003013"
     CDS             7395..7943
                     /locus_tag="ACHOTW_003013"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HFP9845076.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTWRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            7965..8246
                     /locus_tag="ACHOTW_003014"
     CDS             7965..8246
                     /locus_tag="ACHOTW_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9845077.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            8282..9442
                     /locus_tag="ACHOTW_003015"
     CDS             8282..9442
                     /locus_tag="ACHOTW_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HFP9845078.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            9516..12074
                     /locus_tag="ACHOTW_003016"
     CDS             9516..12074
                     /locus_tag="ACHOTW_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFP9845079.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVKDSERFA
                     KWFENARAAEVLHDIEYSDLNEDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSHARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSEATLPETESNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAVARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            12079..13302
                     /locus_tag="ACHOTW_003017"
     CDS             12079..13302
                     /locus_tag="ACHOTW_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFP9845080.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAIAMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMTNMQAGRAK"
     gene            13299..14252
                     /locus_tag="ACHOTW_003018"
     CDS             13299..14252
                     /locus_tag="ACHOTW_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9845081.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGSFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            14263..14970
                     /locus_tag="ACHOTW_003019"
     CDS             14263..14970
                     /locus_tag="ACHOTW_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9845082.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            14954..15610
                     /locus_tag="ACHOTW_003020"
     CDS             14954..15610
                     /locus_tag="ACHOTW_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845083.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            15611..16921
                     /locus_tag="ACHOTW_003021"
     CDS             15611..16921
                     /locus_tag="ACHOTW_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9845084.1"
                     /translation="MSGEILAVNCVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKMRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            16930..17718
                     /locus_tag="ACHOTW_003022"
     CDS             16930..17718
                     /locus_tag="ACHOTW_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HFP9845085.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLMGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            17715..19103
                     /locus_tag="ACHOTW_003023"
     CDS             17715..19103
                     /locus_tag="ACHOTW_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845086.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSFSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            19117..20058
                     /locus_tag="ACHOTW_003024"
     CDS             19117..20058
                     /locus_tag="ACHOTW_003024"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9845087.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMFNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            20055..21041
                     /gene="ycdU"
                     /locus_tag="ACHOTW_003025"
     CDS             20055..21041
                     /gene="ycdU"
                     /locus_tag="ACHOTW_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFP9845088.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            21408..22610
                     /locus_tag="ACHOTW_003026"
     CDS             21408..22610
                     /locus_tag="ACHOTW_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9845089.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT         5916 a         5004 c         5925 g         5820 t
ORIGIN      
        1 gttaagggat gatgtatagc ggtgattgct attagatggg aatgaaagct tgccaaaccg
       61 ttttgctctt ctttctccct ttcaacgata agccaggcat aatgtggctg cttaagccaa
      121 ttgtcgcaaa gccaaagctc tctatatagc taacctcatg gttaatccga tctcagtaat
      181 accatgagca agaactcttt cctttttata taactcttaa gggttacatg cgtgaatgca
      241 aatggtaata taatgaaatg tcgcctgccc gtaatgacca taaagaatct accccgacgt
      301 aaaagatata gagaataaga tgcagtaatg atgatgcttt gttaatcaaa taataagatt
      361 taaattaatt gtaactcacc aactttgttg ttttattcgg atgtctgaaa tgatgtcgta
      421 cagaaaacta catatcattt cagacgtcct ctaaattgga tttttttcac ccaaggagca
      481 aggatggggg acttttaaat tattgtgttt tagtaagtta ggttcttcgc cgttcgatga
      541 aaaaacaata actcttagtt gtttttttga gtccttattt cagcttattt ttcgtgattt
      601 tttattgcgc caggtgatag gatttgccgc aatcattttt gctgatattc cgtgagtcat
      661 caaaagtgat ctgcatgtcc aaaatgaata gcatcaataa ggaaaacgtt agtggcgatg
      721 ataaatttaa gtaaggaagc aacggtgggg aaagcattaa cccctattgc tatacttatg
      781 atgttgtctt ttcctgtagc ttctcaagcg gcgggattag tcataaaaaa tggaacggta
      841 tataacgcca atggtgtgcc agtcgttgac atcaacaaac ctaacggtag cggtttatct
      901 cataatatct gggataacct aaacgttgat aaaaatggtg tcgttttcaa taatagcgct
      961 aatgaatcca gtacttcact tgccggaaat attcagggaa acagtaatct gacctccggg
     1021 tcggcgaagg tgatcctgaa tgaggttact tccaaaaatc cttcaaccat taatgggatg
     1081 atggaagttg caggggataa agcggatctg attattgcca acccgaatgg tattactgta
     1141 aacggtggcg gttcaatcaa tacaggtaaa cttaccttaa ccaccgggac gccggatatc
     1201 caggatgaca agctggccgg ttactccgtg aacggcggta ccattacgct cggtaaactg
     1261 gataacgcca gcccgacaga aattctgtcc cgtaacgtgg tagttaacgg caaagtgtct
     1321 gccgatgagc tgaacgttgt tgctggcaat aactatgtta atgccgcagg ccaggtgacc
     1381 ggtagcgtat ccgccacggg gtcccgtaac ggttacagcg tagatgttgc caaactgggc
     1441 ggaatgtatg cgaacaaaat cagtctggtc agcaccgaga aaggtgtggg ggttcgcaac
     1501 ctcggcgtta ttgctggggg tgttaatggt gtcagcatcg attccaaagg taacctgtta
     1561 aacagtaacg cccagattca gtctgcaagc acgatcaacc tgacaacaaa tggtactctg
     1621 gataacacca ccggtacggt gacatctgta ggcactatct cgcttaatac caacaagaat
     1681 actatcgtga atacccgtgc gggtaacatc tctacgatgg gcgatatcta cgttaacagc
     1741 ggtacgattg acaatactaa cggcaagctt gcggctgcag gaatgctggc ggttgatacc
     1801 aataacgcca cgctgattaa ctctggtaaa gggagttctg tcgggattga agcggggctc
     1861 gtggcgctga aaaccggaac gctcaacaac agcaatggtc agattcgcgg tggctatgtg
     1921 ggtcttgaat ccgctgcgct gaataacaac aacggtgata tccagaccac cggcgatatc
     1981 gccattatca gtaacggtaa tgtggataac aacaaaggtc tgatccgttc gtccaccggg
     2041 catatcgtta ttggcgcggc aggtagcgta aataatggtt caaccaaaac cgccgatacc
     2101 ggcagttctg actctctggg cattattgca gataccggcg tagaaattgg tgcgaacaac
     2161 atcaataaca acggcggaca gattgcgtct aatggcaacg tctccctgtc aagttacagc
     2221 acgatcgacg actatgcggg caaaattctg tccaacagca aagtgattat caagggaagc
     2281 tctctgcgta acgataccgg ggggatcagc ggtaagcagg gtattgaagt cgccgttggc
     2341 ggcagcctga ccaataatat tggcgtgatc agctctgaag agggtgatat ctccctgtta
     2401 gccaactccg tggataacca cggcggcttc atgatggggc agaacatcac gatggagtcg
     2461 atgtctggcg tcaataacaa cacagcgctg atcgtggcca gcaaaaaact gaagataaat
     2521 gcgcgcggca gtatcgaaaa ccgcgatggc aataacttcg gtaatgctta tggtctgtac
     2581 ttcggcatgc ctcagcaaac gggtggaatg gtcggcaagg aaggcatcga gctttccggg
     2641 cagaacatct ataacaacaa cagccgtctt atcgctgagg atggtcctct gactctgcag
     2701 gcgcagaaca cgttcgacaa cacgcgtgct ctggtcacca gcggggcgga tgcatctatt
     2761 caggttggcg gaacgtatta caacaactac gctaccacct ggagtgcggg caacctggat
     2821 atcgacgcga ccacgctgca aaacagcagc agcggtacga tgatcgataa caatgcgacc
     2881 gggttcatag catctgataa aaacctgtca ctggaagtgg tgaatagcct taccaactac
     2941 ggctggatca gcggtaaagg cgatgttgat gtcacggtga ataacggcaa cctgtataac
     3001 cgcaatacca ttgcggctga aaaggggctg gatattgccg cgttgaacgg tattgaaaac
     3061 tggaaggata tttctgctgg cggcgacctg acgatgaaca ccaatcgcca tgtgaccaac
     3121 aactccaaca gcaatatggt ggggcagaat attgttatta acgcggttaa cgatatcaac
     3181 aaccgtggca acattgtcag tgacgctgac ctgaacgtga cgaccaaagg caacctgtat
     3241 aactatctct atatggtagg gtatggggat atcgcattgt cggcaaatag cgtggcgaac
     3301 aataacgcga ccatcgaagc gacaggcgat ctgattatcg attcgaaggg taacgtgggt
     3361 aacaaccgcg gtaatctgca tgcgttgaac ggcgtgttgt ctgttaaagg caacaatctg
     3421 aacaacgata acggtgaaat tcgtggttat ggcgatgtca cgctggcact gacgggcaac
     3481 tacgacagct ataagggttc gctgacctct gaaacgggcg acgtgactct gacggcgaac
     3541 attgtagaca acgcctatgg tttgattgcc ggtgagaatg tttctgtcga tgctaaatcg
     3601 acgatttaca acaacactgc gctgatcgcg gcgaataaaa agctggttat taacgctggc
     3661 ggcaacctcg aaaaccgcga cgggaataac ttcctgcgta ataacggcgc gctgtttgga
     3721 attaccgaca acgttggcgg catcgtaggt aaagaaggtg tcacgctttc tgctcagaac
     3781 gtctacaaca ataacagcag catcatcgct gaaaatggtc cgcttaatct gctgtccagg
     3841 ggaacgctgg ataatacccg cgcgcttctt agcagtgggg ctgatgccat catccgtgcg
     3901 gcagggacgt tctacaacaa ctatgccacc acgtacagcg ccggtaatct cgacgtttat
     3961 gcggcgtcgt tgaacaacgc cagcgatggt cgcctggaag acaataccgc cacgggcgtg
     4021 attgcgtctg acaaaaacct ggatctgagc gttgataaca gtgtcactaa ctatggttgg
     4081 atcagcggta aaggagatgt gcatttcaat gttctgaaag gcacgctgta taaccgtaat
     4141 gccatcgcgg cggacaacgc gctgaccatt aatgccctga acggtgttga gaactttaaa
     4201 gacattgtgg cgggtactgc gctgactatt gatacgcaga agtatgttac caacaacagc
     4261 aacagtaata tgttgggaca aaccatcgcg atcaatgccg tgaatgacat taataaccgt
     4321 ggaaatattg tgggtgatta ttctctgggt gttaaaacca ccggtaatat ttataactac
     4381 ctcaatatgc tgagttatgg tgtcgctggc gtatcggcaa ataaggttac gaatagcggt
     4441 aaagacgctg ttctcggtgg cttctacggt ttagcgttag aagcaaacga aactgataac
     4501 accggtacta ttgtcggcat gtaagatgta accaaagaca gcagtggctt ttaatattaa
     4561 tgcgtcatgt cacgcagggg ttgctgctgt ctttatttag tttgagaaga taatgcaatt
     4621 acgatttaat tttggtttgc tgctcattct ttgttgtttt caggttgcag cgaaagacag
     4681 tcttgataat ttgatcaagc agcaaaaaaa tgcagatgaa agcacgttgc gcgcgcataa
     4741 attagaaaaa aaagatgttt actcaaatgc acaacgtaat gcgtttactc tgggtgatct
     4801 gccgcaggaa aagaattgct ttatcattaa tcggattgaa ctggaagata actttctggg
     4861 tggtcagttg atttctgaag tgcaaaaagc gattgccgga cgttgtgtcg gtagtgctgg
     4921 tgtgcaaaaa attgcagctg ccttgcagga ttattatatt aatgcgggat atatcacaac
     4981 gcgaattagt atcccttcgc aggatatttc taaaggcaag ctacgtttcc aggttaatgc
     5041 cgggaagata gaaaaaacga ttgtcgaagg taatgatatc agtgaatgga tgttaccttt
     5101 tcagcgtaac gatattctca atattcgtga tattgaacag gggctggaaa atcttcagcg
     5161 agtacctgac gttgatgtga aaattaatat tgaaccggga acacgggatg gttacagcat
     5221 cgttcatatt gatacacacc gggggaaaaa ctggagcgtt cgggcaagtt acaacaattg
     5281 gggcgatgaa gaaacgggcc gttaccagac aacctcggtg ggttatctgt ttaatccagc
     5341 gaaaatgggg gatctgtttt atctcgccgg tacgcgtagt actaccggac agtatgaaaa
     5401 cgtcagtagt tattattcca ttccggtggg gtactgggag tacggctttt tctatagcaa
     5461 aagtaaatcg cagcaggtga ttccgctttc gtacatctcg ctggattatg ttggcatcag
     5521 cgaatacttg agcgcaaaag cgacgcatac cgtttatcgc gataaaacaa aaaagttcgc
     5581 cggtagtgca gaactcattc gacgaaagtc gaattacacg cttaatggtg aggagctgac
     5641 attacaggcg cgggatatgg gaaatatcaa gctggggcta aattataagc aacagttacc
     5701 cggcgcgttc tgggattcaa ccctctcgtg gcaacgcttt ttaacctggt tcggtggtga
     5761 aaaaacgccg gatatgcaat atggcgacgt tagcccggta agcaatatct ttaattttga
     5821 gggtaactat acccgacaaa tagagaatgg atattacaat accgcttttt ttgcccagta
     5881 cgcaccgcgt gaactgactt tgcaagataa aattacagtt ggcgatcgtt ggagtattcg
     5941 cgggtttgaa aatagcgtag gtttgtcagg gaatgatggt ttttatataa aaaatacgct
     6001 ggcatttcct ttgcctggaa tgaaagctaa ttattatgcc ggtcttgatt ttgggcaagt
     6061 gtatcaggac gcttcttatg gcgatgaatc actggtgggc tcggcagttg gtattgatgg
     6121 aaacattaaa agtcttgagt ataacttttc cgtttctacc ccattaaagt atccggcgac
     6181 attagatatt gatcgggtga atgtaaattt taatttttcg taccaaatgt aaaggataac
     6241 gagactcatg agaataggga cagatattgt tgaaattgcc agaataacca aagccattat
     6301 taatggagag atggcttttt atgagcgtgt ttatacttct catgagcgct tactcattaa
     6361 ccctgcagaa cctgattatg aacgggcctc tggattctgg gcggcgaaag aagcgttagt
     6421 taaagcgatt ggttgtggtt ttcgtgatgg cgtacgtttc caggatattg aaataaagca
     6481 tgatgcagcc aaagcacctt atttcgttat ttccggaaga ctacaagaaa tttttatcgg
     6541 gcaggggttg caaaataact ccctgagtat ttcgcattgc cgtacccacg ccattgcgac
     6601 tgtcatcgtt tattaactca acatatgtga tgaatatgta caaatataat gatttaaatg
     6661 aaaaaaccgt actggtgaca ggagccagtg gtgatattgg actgggtatt tgcgaaaaat
     6721 atctggagca gaactgcgat gtttatgcgc tctataaatc caacgacgtg caattaacgg
     6781 cgctgaaagc aagccatcct gcaggcgata aactgcatat tgtgcagtgc gatcttgcct
     6841 gcccccagag cgtgagcgca ttgtgcgaac agatagagcg ccaagctggc aagattgatg
     6901 tgctggtaaa taacgcgggc atcgttaaag acagcctgtt tgcctcgatg agttatgagg
     6961 atttcaccca ggttatcgaa accaacatgt tcagcatttt ccgtctgacg aaagacgcgc
     7021 tgatgctgct tcgtgcggca gaaaatcccg ccattattaa cgttgcctct attgcagcgc
     7081 tgatcccaag cgtcggtcag gcgaattaca gcgcgtcaaa aggggcgatc ctaggcttca
     7141 cccgcacgct ggcagcagaa atggcaccct ggggcgtgcg cgtcaacgcg gtcgcaccgg
     7201 gaatgattga atcgaaaatg gtgaaaaaag tatcccgcgc agtggtccgt gcggtaacca
     7261 gtaccatccc gttacgccgt ctgggtaaat gtgaagaagt ggcgaacacc atcgtcttct
     7321 taagttcctc tgcctccagc tacattgtcg gccagaccat cgttatcgat ggcggcctgg
     7381 tcatgcggtg atttatgtct aaatacacga ttccttcgaa aatcttcctc gaaatgggcg
     7441 gctggcgtca gccgctgctg atggtcgata aaattgcaga ttataaatac ggtgaaaatg
     7501 gctttgtcag tgtcgtgaaa cacgtcacct ataacgaacc gtatctactg gggcatttcc
     7561 cggaagatcc gattatgccc ggggtgatta tttcggaaat cttcggccag gccagcgagt
     7621 atctctcttt cctgaccgac atttgcgata tctggcgcga acgttttgag gaagagctga
     7681 agtcgttacg tgatattcac gcacgcattc accgccctga aatgctggag attatccgta
     7741 cctggcgcag ccaggtacgc ggcgtgctgg cggctcagaa cctgaaattt aaagatatcg
     7801 cctatccggg tgattctatt gatgttgtca gcaaattggc tttttcggat gccagtggct
     7861 ttaaacacta ttccgtgacc gcgtacgtcg gtaaaaagct gattagtcag ggcaccatta
     7921 tcaatttccg tgaaaccaag taatccaata agagagtaaa tattatgtct accatgaatg
     7981 acgctattga acagcgtaaa gaagttctga tgaccattaa aactgagatc atccagcgac
     8041 tgaatattca acgcgatgaa gcgcaaattg atgatgatac tgcgctgttt ggtaatggtt
     8101 ttaaactgga ctccgtcgat gcaacggaaa ttgtcgttct gctggataaa gtatttgata
     8161 tcaagatcag cgaaagtgac gacccgtcct atatgcgcag tatcaatagt ctggccacct
     8221 tcgttattca aaagaaaaac gattaactac ttattaaaga attaattatc aggacacgtt
     8281 tatgaaatct cttgctgaat ttcacttgaa aaataatgcg gtaatggggg tatataacaa
     8341 ccgtacccta ccttcttctt atcacgatgc tatgaccgaa tataaagccg ttcgtgaaaa
     8401 cgcgctgctg gtggactatt ctcacctctc tattgttagc gtcatgggcg atgatgcctg
     8461 ggcgctgatc aaccagctgg tttccgcaga tgtctccatc attcgtgatg agcaggcaat
     8521 ctactcactg gtgttgaatg aagagggcac cattcgcgga gacgtctatg tgctgtgcag
     8581 cattgacggt tattacctgc tttcagaaga tatttcggct gccgaattga tcgccagcat
     8641 gaataccatt ctcgaaaaag cggaagaact ggatattcaa tcgatgcctg aaattcagga
     8701 tatgcgggaa aacaactggg gggcaatcct gctcgaaggc ccatactcct gggagatcat
     8761 gtctgaaatt catggctttg acgtgattgg cctgccttac tacgaataca tgaataccga
     8821 ggaggatctg ctgctcttcc gctgtggtaa acacggtgag tatgcgtaca tgaccatcgg
     8881 tgagcaggcg aaactggcgg agcagtggga aaaactgtta accgttggcg agaaatacct
     8941 gatgcagacc ggcggcctgg attatcaaaa aatcgtacgc ctcgaaaatc cgtgctggga
     9001 tgccagcctc tgggaaggtc aggcggtgaa tccggttcag ttgcagatgc agtgggcggt
     9061 gcagtacgac aaagatgatt ttattggcaa ggacgcggtg acagagctat cccaggagta
     9121 taccggtaat aaactcatcg gcatgatcgc acaggaagaa tgcgaaggca ttgaggctgg
     9181 cgatcgcgtg ctggtggaag gtcaagacgt gggctatgtc gtgaaggcac tcttttcccc
     9241 agcgttgcag cgtttcattg ccctgaccct gctggaaaag gattacgcct ggtctgacat
     9301 cagcggctac gaaattcaaa ctgctcacgg aattattccg gcgcaatcaa aatgtatgcc
     9361 gtttatttat aacctgagca tgttggttag cccaactgag cacagttata tcgacgcgtc
     9421 aaaaaataaa agcgcagcgt aatgaatatc gccgcgtgac tatagcttac gcggcttaaa
     9481 ttaaccccaa ttggcgggat tgccaggtat tattcatgaa aacgaacagg aaaagagttg
     9541 ttattaccgg gctggggatt ttgtcctcac tggcggaaaa tattcaggat ttccgggaag
     9601 cattattaaa taaaaaaaat ggcgttaagg atagcgaacg ctttgcaaaa tggtttgaaa
     9661 atgcgcgtgc ggcagaagtg ttgcatgaca ttgaatactc cgatctgaac gaagatgtta
     9721 tcgattcact ggataacgca gcgctctggg cctacaaagt gggtaaagat gcgctgagtc
     9781 aggccggcct gatggatagc cgtgacaccc tcaaagaaac aggcatgatc gtcggtgttt
     9841 cgtccgcggg aacagaggca tttttgccgc tgttcgaaca gcgcatggaa gatttctcgc
     9901 tgcgtaaagc actcttttcc ggcgcattct catcctgttg ttccagcgtt tcaacgctgc
     9961 tgggcttaca gggtggggtt gaactggtgg cgaccgcgtg taccgccagc ccgaacgccg
    10021 tggggatggc ctttgactac atccagaatg gtaaaagcaa aaccatgctg gcagtgggca
    10081 ctgagcccat ctatctgccg acctttgccg gattctacgc actgaatgtc atgcatccag
    10141 acacttgtac gccgttctcg ggtcagtccg gtatgtcgat tggtgaaggc gctggcgcta
    10201 tcgtgctgga agagtatgag cacgccgtag cacgcggggc gaccatttat ggtgagatcc
    10261 tctcttacgc cacttcctgc gatgctttcc atgaaaccgg gccagatccg cgtgccagcg
    10321 gagcggtgca ggtcatgaac aaggcaatgg caaatgcggg cgtaaccccg gagcaaatcg
    10381 attacgtcaa cgcgcacggt acgggtaccg aagccaatga ccgtattgaa acgctggcaa
    10441 tgaaaaaggt cttcgctaac cacgataaac tgctggtgag ctccaccaaa tcttactttg
    10501 gccacaacat tggcgcagcg gggattgtgg aactgattgc ctgtctggca acgcttccgg
    10561 ataaccgcgt actgccgacg ctgaatttca gccatgcacg cccgaattgc gatctggact
    10621 atgtgccgaa tgagtttcgc gatcgcaaag tgaacatctt catgaagaat aactatgcct
    10681 tcggcggcaa caactgctgc atgatcctga gcatggagcc cgcgtccatt ccggtcagta
    10741 cctatgattc gaagcgtgtg gctatctcgg gcgtcggtgc agtatccgca ataggccaca
    10801 cgctcaacca ggttctggaa aacatctggg cgcaggacca ccacgttgaa ctgggtagcg
    10861 taaccttccc ggacgataca ctggaagaag ccaaagagct gctggccgtg ctggatgaat
    10921 caaaccagtt tgccgagctg tttgatgaag agtacagctt cagcgaagct acgcttccgg
    10981 aaacggaaag caactttaaa acgttccagg tttccgggct ggagccgcgt aaacacctgc
    11041 gtcgtttcga tcagcgtaaa gcgacccgtg gcggcacgtt tgccctgatc gcactcaccg
    11101 aagcgctgga acaggcgaag cgtaaaatca aacgcgacgg cgatgagttg ggcatgatca
    11161 tggggatgtc ccgcggcccg caggaaacca cctacaaata tctgcaaagt ctgaaacccg
    11221 atccacgcaa agtacgtaca tcggaatttc ctggctcgct gatgaatgcg atcccaacgt
    11281 tctgcgggat ctctgaaggg atcaaagggt ataccactac gcttgcgaca ggggaaaact
    11341 cggcgctggg cgcgctgacc tacggttatg aaatcgtccg gcagaacctg caaccgcagg
    11401 tattggttgg cggcgcggat gagtacttcc cgtcgatgtc gctgtatatg gatgcggtga
    11461 cacaaaagat ccacatgacg gctgaggcga gcgactatca gatttacggt aaagatccgc
    11521 aaggttatgt tccgggcgaa ggggcttgca tgctgctgct ggaagatccg cagttggctg
    11581 tggcgcgtgg cgctgaggtt ctcgcggaag tggtgggcta cggcaagtca tgcagcaata
    11641 cctacttcga cgcctcattg ttggaagaaa aatccgcgtc gatggcgctg gccattgaac
    11701 gcgcccttac gcatgcgggc atctctgctg gcgatattga tttggtatgt ggtaccagca
    11761 acggtagcga tgaccattct cgtatcgaaa ttgacgcgat ttacaacacc ttcgccgcgc
    11821 aaaacccggc ggtgccggta gtgaactata acgcttgctt tggttttgtc gcctctgccg
    11881 ctggtttgct taacctcgcc gtgattactg actgcattaa gaagcaggca gtacctgcga
    11941 ttccaaatac ggtggaattc tttgatgagc gtgtgaattt tgtacgccag tcgatgaaac
    12001 ttgcgctgaa ccatgtcctg cttgttggcg caacggaagg cggcaactac tacgcgttcg
    12061 tcattaaggg ataagactgt ggacaatgcc gctattattt cgggttacag cgtgtggatg
    12121 ccgtatgccg aagactgttc gcagttaatt gacaatctga aacaggggaa acgcgttgcg
    12181 cgtacaccct ggtttacctc caacgaggaa gccattaaat gcgggtttaa gggtaatccg
    12241 tcagtggcga cgttgaaaca ggtggacgat agcgcattgg acctgctgtc tcagttgatc
    12301 gacgaagccc tggaacaggc aatgctggat aagcattgcc tggccgggcg caacgttcgc
    12361 gtttatctga ccggtattgg gccgcgtatc gacgggctgg attacaaatc tttctataat
    12421 tacaacgatg tagaagatat caacttaacg caatccatca caaatctgca tgcctcaaag
    12481 atgtcgcagg acacgatttc aagccatctt gcgcgcaaat atcgcttgca gtatttgccg
    12541 cctaacatga actgcaccag taactcgtca ctgactgcgg tgcatctggc gacgcagggc
    12601 attgagcagg gcgggattga tctcgcgatt gtgctgaact gttcgaaaat taaaacccag
    12661 gacatctggt tcctggaaac gcaatcgatg ctggacagcg aacaagtgca gccgtttggt
    12721 gaaaacagta aaggtgtggc gttcgcagaa ggttttagcg cgctcctact ggaaagcgct
    12781 catcaccgcc gggcgcgtca acaaagtgag ggcgtaaggg tgcaaacgac ttacacccaa
    12841 atcagcgcgg gccgcagcaa cgatgcctcc tggcttagca ctaacgtgca caaggtcatg
    12901 caggccgcga tgaagcaggc tgaaattgcg ctggatgatc ttgcagccat tcttccacac
    12961 ggcaacggtt cggcagtgag cgataacgcg gaggccaaag ccatcgccat gttcgcaggg
    13021 gagcgacaga tccccgttct cgcctataaa gggcagatcg gctataccgc aaccggatct
    13081 ggtgttgtcg atctgatcat tggccaccat tcgttgacgc atcatcaact gatcgcacct
    13141 gttggcaacg acgtcattat cgacagcatg gcttcgctgg tacttacgga cggcagcgtg
    13201 acaaaccata gcaaacgcca tttgctgaag gttggtgtgg gtgtcgatgg ttcagttatt
    13261 ggcgttgtca tgacaaatat gcaagcgggg cgcgcgaaat gacggaaaag agtatctacc
    13321 tgtcttcctg ggcgcttacg gaaccggacg acatttttta tttttatcag ccgacctggc
    13381 tcaacgcctg ggaagcacat atgtacgaaa ggggattata cggcacccgt gctaatggct
    13441 ggggactgaa gcgctttggc gtggagaacg atatttatgc accagaacgg atccgtaatc
    13501 cacaatatat gccttacctt gaggcgatgg agcaggtgac cgattctgca ttacaacaaa
    13561 tgccgactaa agaacgcatg aagttaatga aatcgcgcac ggcatttatt tatgccgatt
    13621 cctggggcga atccggttcg tttgaaaaaa tttccagtgc gctgcatatc gccactattg
    13681 atacgcttcc caagaattta cttaagaagt tttcgataac ggaaccgact tgcaagattc
    13741 gcggcgaaaa acaggcattc gttcaggcgc tgcgtatggc gcaggattat ctcgcctggg
    13801 atgtattcga taacgtggtg atttgtgctg cctatcgggc aattccggtg ctggtattct
    13861 ctgaagaaga tgtgggtaaa ccgcctaagc gtttatttcc ccgtcgtgct gatgaggtga
    13921 atttatctgt tgaacgagtg ggttgtttta ttctcagcca gcgtgagagc gccatcaggc
    13981 tgcaatgcgg ggaatatcat tccggcactg cgccacagca tgctacggat attgatgtca
    14041 tggccttcag cgaacggcaa caaatgccgt cccggcctgc tgctggcgtt atcgacctgg
    14101 tggcccatta cggtgaaagc ggttgtctga cgcccgcgtt gagtatggat tatttaaccc
    14161 gtcatattca gcccggtgga aaaatgcgta cggtcattgc cgataaacag tttggttatc
    14221 actactttga ccttgaatac cttgaaggat aacgacagtc gtatgcaatc tactattaaa
    14281 tcgttgcccg ctatttcgct tagcaatatt tataagtgtt atggcgctgg ggcaggaaag
    14341 gttgatgcgc ttaaaagcat taatcttgat atcaataaag gcgaatttct cgcactgtgt
    14401 gggccgtcag gcagtggtaa aagtactctg ttgaatattc tctcgggtat tgataaaccg
    14461 accagcggaa cggtgatatt tttaaataaa ttactgaacc aactgccaga agagcagttg
    14521 gctgtgattc gtggaaaaca tttgggtttt atttttcagt tttttaacct tatgccggta
    14581 ttaaatgttt tcgataacgt ttatttccca ctggtgctta atggtcagtt cagtaaaaaa
    14641 gaagccactg aacggacgct gcattatctc gatagtgttg gattaagcgg ttttggcgat
    14701 cgtaaacctg ggcaactatc gggcggtcaa caacagcgtg tcgccatcgc cagggcactg
    14761 gcccacgaac cgatggtggt cattgccgat gaaccgacgg gaaatttaga cctggcaacc
    14821 ggcgaggcta ttttggacct gttgttgacg attaatcagc agaccggcac cacatttgtt
    14881 atttccacgc actccagcga attaaaagcg cgggcacggc gagtggttga aattcaggat
    14941 ggagtattga ttcatgactc agatgcataa aggatttttg ctcatcggcg tctttttgta
    15001 cgcggcgttt gtggctccga agatttacgc ggcaacaccc gacgctgtta accaggttct
    15061 gcaacagggg ctcccggcgc agcctgccgc gctggcgacg ctgaccgagg cgttaaagaa
    15121 agagtatgaa gccaggcaca atgtggtggc gctggttttt tacgcctatg gcttactgcg
    15181 tcaagcggac ggttatgcgg caacaaacga ttttatccat gcctcagaat acgccaaaag
    15241 cggctttttc tggctggatg aagcggtgga tttgcaggag aaaaatcaac gcgtacggta
    15301 tttacgcgcc cgcgtagatg cctatttacc cgcagactca gggcgttgcg tggtgacagt
    15361 caaagacacc gaacacatgc tggctgaccc ggcgatatgg acgacaacga tccgcgacca
    15421 tattctggcg atgcgttatc gcgcattacg tcactgcaag gacaccaccc gcgcgaatgc
    15481 gttgttggcg caaattaaag ggcaaaacgc cgcgcttgcg cagtcgctga cgcaggattt
    15541 taatgttgta ccagaatggg atagtgaaga gttaacccag gtactgctgc cgctgatgaa
    15601 aggtgagtaa atgtccgggg aaattttagc tgttaattgc gttctgctgc tgattctgtt
    15661 ttttgtgttt tatcagcttc tgttttattc acgagatgtc aaatttgcat ttttgaatct
    15721 ctttcgccac aagcgtcgct ctttttctac cataaccgct attgtattgg gtggcgtggc
    15781 aattttttta tatggcggat ttattgatta ctctttctgg atcctgaaag agcagacgat
    15841 tcgtaccaat attggtcatg tacaaattta caatcagcac tattttgaaa cgtcgaataa
    15901 aaataagagc ctgattgccg attatgcggc gttaaagaaa gctattttgt cggacccgac
    15961 gctggcggcg gatatttcca cgctctccgg gcaactggag tttactggcg taatttcaca
    16021 ttacgagagc gaaacctcca gctatttctc cgcgctgggg gtcgagccgt tacccgcttt
    16081 aaagctgggt tcgttcgata agattatcgc ggggagcgat ctttcccggg taaaaggtga
    16141 tgaaatcacc ctgggcagcg gtctggcgaa aacgttgaat gcgaaatatg acgactggct
    16201 ggatgtgatg gtggtcaaca cggcgggcgg gcagggcgca ttgtcgctaa aaatgcgtgg
    16261 catttttgag tcaggcatta aagattatga cgatgtcgcg atgaagatcc cgctggacac
    16321 cgcgcagcgc atgatgggta cggatggcgt cagcaaagtg cttattctgc ttaaagagga
    16381 tgacaccgcg gcgtttagcg caaagctgcg tcaatttatc gaacgcaatc atttgccgct
    16441 ggtggtcaaa gactggcgtg atgtttcact cttttatcaa caggttgaag ggttgctatc
    16501 ggggatctat ttcttcatca agctgattgt tgcgctgatt gtcatcttca tgatcggcaa
    16561 ctcgatggca atgaacattg ttgagcgcac ccgtgaaatt accactctgc gtgccatagg
    16621 cctgaagccg cttcacgtca cgcgcttgtt tctgacagaa ggcattttta ttggtgtgat
    16681 tggcgcagtc ggcagcctgc tggtgggggg cgtgttagcc tggattatta atctttatgg
    16741 tattgcgatg ccgccatcgc cgggacaaac catcggctat accgccttta taaaaaccag
    16801 taacccggaa ttgatttggg tgacggtggt attacctatt ttgacagcaa caggtgcctc
    16861 tgtgttaccg gctttacgtg cttcccggct gaatatttct gacgcattta aattctcata
    16921 gggctaatta tgaatatggc tttttacggc aagtggtttg cttgcctgtg gctcgctacg
    16981 tcgtgcgttc aggcggcgag cacagataat aaggcgctgg agataattcg tcgcgctgac
    17041 gaaattcgtt cgccgaataa accgtttcgt tacacgctga cggtaacgga atataaagcg
    17101 ggcgcaaccc agccagagaa taaacaggtc ctcgatattt ccatgcgctt tatgaaaccg
    17161 cagggtaatg aaaaagcgga tgcacgctcg ctggtgcgtt ttatttatcc gccgcgagat
    17221 aaaggaaaaa tcatgctttc cgactggtac gatttatggt tttacacgcc ggaactgcga
    17281 cgtcctatgc ctatttcacg gcaacaaaga ttaattgggc aaatctctaa tggcgatgtc
    17341 attgtcacta actttgaata tgcctatgac tcgaccctga tgggggaagt cacgtgtgct
    17401 gaaaagcagt gttacaagct ggcgctggtg cgtaaatcgg cggatatcac ctggccgaag
    17461 gttatctatt acgttgaaaa agacggtgat aatcgcccgt ggaaggcggc ctattattcg
    17521 caggatgacc agttaataaa agaagtgttg tatcaggatt tccagccggt gttggggaaa
    17581 acgcgaccca tgaaaataac cgttaccgat gttcgtcacg gcaataacta ttcagtgatg
    17641 gagtacagcg atgttcgtct ggaatcgctg ccggagtttc atttcaccaa agagtatatt
    17701 cagcgaggtg cgaaatgaaa cgtacgctga agatctcttc gttgctgtgt gttgcgctgc
    17761 cgttgacggt acaggcggat tgcctgtcag gtgacgaggt ggcacaaaat agcgatgtga
    17821 cgctttacga gcaggtgtct tatgtcaata aacaggcttc ggcctggcag attgcaggca
    17881 aaaatcccta cacccgtcac aacggttatc aggaggcggg gattggcatc aatagcggtt
    17941 gttcgataat cgacaatacg ctggacctta agctcaatct gtatggaata aacgaatatg
    18001 cgcttaaacc cgcagggaaa ttcgaaacgg atgatagccg cacgcgtgcg ttaattaacc
    18061 gcctcagtct ggtctatagc gcctcggaca gcgttcaatt tgaagcaggc aaatttgccg
    18121 cgccgtcagg aatgttcttt ttgcgttcgc cttctgattt gcaaacgcat tattacaccg
    18181 gattccagtc gacgcgtttg cacgatccca aaatgacatc ggcgtatcag gcgtcatcgt
    18241 ggggcgcaaa aatgagcgtg gatacacgcg attatgcgtt ttcggcgagt gtcataccga
    18301 agttagccac catcgacaaa cgctatgtga cgtctggcaa ctggtcggct aatcagcagg
    18361 gaaacagcga cgaagcctat ctgctgagct tcagcgatca tcgctttggt gagcacacgc
    18421 cgacagtcaa tgtgcggctc ggaccatcgc cgtcactcgc gctgtctgac agttatcact
    18481 acaccccgca gcttacgctg aatgtcgacg cagcctacca ccgctcccag caatggcggc
    18541 atctttccca ccgtgagacg gcgcaggtcg aggaatatca gtttccggat tcgctctatg
    18601 aaacgaaaga tgaaagcggc gtagagctgg cgctgggtgg gcagtacacc agtgataatt
    18661 tcagcgtgtt tggcgttgag tattactttc agagcgaggg ttattcgcgc gctgaacagc
    18721 gtcagcagcg cgagttaatt gacttcctca ataccacaac cggctatgcg ccactggatc
    18781 aggcatttga ttcttacaaa tacctgatgg cttcagaaat cagcaatacc gccaatcagg
    18841 ggatgctgca gggtaagcac tatctgaacg cctgggccag tctgccgctg gcgggagagt
    18901 cgacgctgca gccttcgctg gtggtgaatc tcgttgatgg cagcacgttg ctgggcctgc
    18961 attattccac gccgctcagc gctataagta accagcttga agcctatgcc ggtggctata
    19021 gcgcgctggg aagtcggtac tccgaatttg cgctttttgg cgacacgtta ggcctttatt
    19081 tgggctttaa atactatcta taaggataaa gagatgatga ataatgcttc acaaccgaca
    19141 gtactgcttt ttgccggtca gggtaatccg gttattggta tgggcagcga tctttgggat
    19201 ctcaacgcca gtacaaaaca tatctgggat tgtgccagcg atatttccgg actgaacctg
    19261 cgtcgcctgt gtcagaaagg gccgatgaat cgccttattc aaacgacggt acaacaactg
    19321 gcggtgaccg caattaatgt aacgctctat accctgtgcc gcgagcgtct tgagaattta
    19381 acggttgtgg gctcctgcgg acacagcgtt ggtgaataca gtgcgctcta cgccgctgga
    19441 gctatttctc ttgagacgct atttggcatg attcacttca gagccagcct gatgcatgac
    19501 gaaagccaga gaaataaagg cgcaatgctg gcggttaaag gcatcaaccg cgaacaactg
    19561 cagacgatga ttacggaatc aggcattgcg ctggatatta gctgtgataa cacgccgcgc
    19621 cagcaggtta ttgggggaac gcaggctgcg ttgaatgaat ttgccacgct gctgatggcc
    19681 gctggatacg aaccggttaa actcggtgtg agcggtgcct ggcacacgcg tttgatggaa
    19741 gacggcgttc aggcaatgcg cgattatctt gccggactgg atatcgcttc gccagagcat
    19801 caggttctga tgaacgtaac agcaaaaagc gaggtcgctc cgtcaattat caaagaaaat
    19861 ctctcattac acttaactca tacggtgaaa tggactgaat ctctcgatac atttctgaat
    19921 atgccaacct cggtagcttt ccttgaaata agtaataagc cctatttggg gaatatgttc
    19981 aacgatttcg ctggcgtgga tcagcaacga gttatgcatt gtcgtaaagc atttagtgat
    20041 gcgaaggtat tcaaatgatt cctgattatt taacttttat tcgctttcag gataaacgaa
    20101 gtctgatata catttatgct attggactta ttctgatagg cttttattgg aaaaatgcag
    20161 gatttacttt tccatcagag gatattggtg tcgttagtgg gattctggcc ctggtgctgt
    20221 ataattttat ttttgatctc aaggcgtact gggcttataa atgcgtcacg aagaatatcg
    20281 atttttcgtg gtttaagaaa aagcagaacc acaaaataga attatttctt acgcaacctc
    20341 tggtggcagg atttctgtcg ttaatcatgt tgagtgcaat gagttggggg ctataccagc
    20401 gtctaccctc gttatatgcg ctgttcctga tttcgttact tgggccgttg gttatctttc
    20461 tgctgtttcg gatgatccgc accagttatg tcaagcaggt cgctatttca gtagcgaaaa
    20521 aagtaaaata taaaagtctg actcgctatg tgctgctttc ggtgtgcatc tcaacggttg
    20581 ttaacctgct tactatcagc ccgttgcgta acagtgattc ttttgtgata gaggggcagt
    20641 ggttaacgtt taaatcgata attgcattgc tcattctttg tggcgtagtg ttggcgatta
    20701 atctgttttt tctgcgcttc tccaggcggt acgctttttt gggcaggctt tttttgcagg
    20761 aaatcgatct ctttttctcc agtgaaaatg cattgtcgac cttttttgcc aagccgcttt
    20821 ggcttcggtt attcatattg ctggttattg aagtgatgtg gattacgctg gtgtcggtat
    20881 tggcaacgct tgtggaatgg cggatttggt ttgaagccta ttttttactc tgctatgtac
    20941 cgtgcttaat ttactatttt ttctattgtc gattcctctg gcataacgat tttatgatgg
    21001 catgtgacat gtatttccgt tgggggcatt ttaataagtg aggaagtgat aggaagtgac
    21061 cagataatac atatatgttc tgtactctct tgcgcatttt gattgttgac tgagcaacca
    21121 gacagttgat gtgcacgatt tcccctcgcc caaacagacg tgggcggggg cacccggtgt
    21181 gtgcaatata cgaaaaaaaa gcccgtactt tcgtacgagc tcttctttaa atatggcggt
    21241 gagggggacc gcctgaaata aatctgactt aatccagggg tgttatataa tttcattata
    21301 aacaatgtat tacacttttt gttgtccgat attatctaat gtattcttag tgaagcgaga
    21361 gactaatggc atactggatg gcataccaga gtgacgggaa ttagcgtatg gcagtattga
    21421 cggatacgaa agcaagacat atcaaacctg atgacaaacc attgccccat gggggaatta
    21481 ccggactgac ccttcatcct tcttcagtaa aggggcgggg gaaatgggtt tttcgttatg
    21541 taagtccggt gacacaaaaa agacgtaatg ctggattggg aacttaccca gaggtcagta
    21601 ttgctgaagc tgcacgtact gcccggataa tgcgagagca acttgctgca ggtgatgatc
    21661 ctctggagat taaaaaggct gaatctgaga aagtcgctat cccaacattt gccgatgcag
    21721 ccaggcgtgt acatgcagaa ctgtctcctg gatgggaaaa tccaaagcat gtaaggcagt
    21781 ggttatcgac gcttgagaat tacgcgtttc ctcaactggg agcaaaaacg ctggattcga
    21841 ttacggctgc ggacgtggca gaaacactgc gtccagtctg gttaaccttg tcagaaacgg
    21901 caagccgggt taaacagcgc attcatgttg ttatgcagtg gggatgggcg cacggttttt
    21961 gtgtagcaaa tcctgttgat gtggttgacc atttgcttcc tcagcagaca agaggacgtg
    22021 atgaacacca acccgcaatg ccctggaggc agttaccgct ttttgtggcg accagtgtgt
    22081 ataccgatga accttataat gttacccgcg cactgttatt aatggtgata cttacagcaa
    22141 ctcgctcggg cgaagccagg ggaatgcgct gggctgaaat tgattttcat aagcgggtat
    22201 ggactatacc tgcagaaaga atgaaagcca ggatacagca tcgtgttcct ttatcccggc
    22261 aggctattta cattctggaa aatatacgtg gcctgcatga tgaactggtg ttcccttcac
    22321 ccagaaagca gcagatcctt tccgatatgg tgttgacaag ttttctgcgt aaaaagaaag
    22381 ccgtcagtga cattccgggg cgagttgcca cggcacatgg ttttcgctca acattcaggg
    22441 actggtgtag cgaacagggg tattcgcggg atctggcgga aagggcgctc gctcatacgc
    22501 tgaaaaataa ggttgaggcg gcatatcatc gtactgatct actggagcag cgtgtaccga
    22561 tgatgcaggc atgggcggat tatgtgatgt ctcaaattgt gaataaataa tagtgaaaat
    22621 gtatattgta cgatcatgat ttgatatgtg atcgtagcag tagta
//
LOCUS       DAZKIF010000082        22323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZKIF010000082 DAZKIF010000000
VERSION     DAZKIF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.082"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            228..896
                     /locus_tag="ACHOTW_003027"
     CDS             228..896
                     /locus_tag="ACHOTW_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9845090.1"
                     /translation="MFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNKIDLLE
                     KQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFILNN
                     GSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEFARWQQ
                     LLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQK
                     RSKK"
     gene            940..5706
                     /gene="ehaG"
                     /locus_tag="ACHOTW_003028"
     CDS             940..5706
                     /gene="ehaG"
                     /locus_tag="ACHOTW_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFP9845091.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            6074..7729
                     /gene="lldP"
                     /locus_tag="ACHOTW_003029"
     CDS             6074..7729
                     /gene="lldP"
                     /locus_tag="ACHOTW_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP9845092.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7729..8505
                     /gene="lldR"
                     /locus_tag="ACHOTW_003030"
     CDS             7729..8505
                     /gene="lldR"
                     /locus_tag="ACHOTW_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP9845093.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8502..9692
                     /gene="lldD"
                     /locus_tag="ACHOTW_003031"
     CDS             8502..9692
                     /gene="lldD"
                     /locus_tag="ACHOTW_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP9845094.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9878..10351
                     /gene="trmL"
                     /locus_tag="ACHOTW_003032"
     CDS             9878..10351
                     /gene="trmL"
                     /locus_tag="ACHOTW_003032"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9845095.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10404..11225)
                     /gene="cysE"
                     /locus_tag="ACHOTW_003033"
     CDS             complement(10404..11225)
                     /gene="cysE"
                     /locus_tag="ACHOTW_003033"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP9845096.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11305..12324)
                     /gene="gpsA"
                     /locus_tag="ACHOTW_003034"
     CDS             complement(11305..12324)
                     /gene="gpsA"
                     /locus_tag="ACHOTW_003034"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9845097.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12324..12791)
                     /gene="secB"
                     /locus_tag="ACHOTW_003035"
     CDS             complement(12324..12791)
                     /gene="secB"
                     /locus_tag="ACHOTW_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP9845098.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12854..13105)
                     /gene="grxC"
                     /locus_tag="ACHOTW_003036"
     CDS             complement(12854..13105)
                     /gene="grxC"
                     /locus_tag="ACHOTW_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP9845099.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13247..13678)
                     /gene="yibN"
                     /locus_tag="ACHOTW_003037"
     CDS             complement(13247..13678)
                     /gene="yibN"
                     /locus_tag="ACHOTW_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9845100.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13923..15467
                     /gene="gpmM"
                     /locus_tag="ACHOTW_003038"
                     /gene_synonym="pgmI"
     CDS             13923..15467
                     /gene="gpmM"
                     /locus_tag="ACHOTW_003038"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP9845101.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15501..16760
                     /gene="envC"
                     /locus_tag="ACHOTW_003039"
     CDS             15501..16760
                     /gene="envC"
                     /locus_tag="ACHOTW_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP9845102.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16764..17723
                     /gene="yibQ"
                     /locus_tag="ACHOTW_003040"
     CDS             16764..17723
                     /gene="yibQ"
                     /locus_tag="ACHOTW_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP9845103.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17710..18745)
                     /gene="waaH"
                     /locus_tag="ACHOTW_003041"
                     /pseudo
     CDS             complement(17710..18745)
                     /gene="waaH"
                     /locus_tag="ACHOTW_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(18984..20009)
                     /gene="tdh"
                     /locus_tag="ACHOTW_003042"
     CDS             complement(18984..20009)
                     /gene="tdh"
                     /locus_tag="ACHOTW_003042"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9845104.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            complement(20019..21215)
                     /gene="kbl"
                     /locus_tag="ACHOTW_003043"
     CDS             complement(20019..21215)
                     /gene="kbl"
                     /locus_tag="ACHOTW_003043"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9845105.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21490..>22323)
                     /gene="yibB"
                     /locus_tag="ACHOTW_003044"
     CDS             complement(21490..>22323)
                     /gene="yibB"
                     /locus_tag="ACHOTW_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFP9845106.1"
                     /translation="TAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENEMII
                     FTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKNP
                     EYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDE
                     SKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITL
                     NNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSR
                     K"
BASE COUNT         5709 a         5753 c         5622 g         5239 t
ORIGIN      
        1 taataatcat cattagaatc cacgaaaata atatattcat acaaacacag acatcttttg
       61 aaacgaatga tacattttta taatgaatgc ttaaaacaat atttaatcga cacacatcac
      121 tgtcattaat gtaaagcaac atagatcacc aataatacaa tggacaactc taccggcatt
      181 gatcgctggg cggaggaaat aaggattttt acatgaacta gaagaagatt tttttcagtg
      241 ctgtcactgt ttcagttctc tgcgcattaa cgggatgtga ctacattgaa gaaggcaagc
      301 cagaaagtag tttattgaaa caacaagaag aacataacaa taaaattgat ctacttgaaa
      361 aacaacaagc acagctaaag agtcaactgg aaactattca aaaacaacaa actggcataa
      421 taagcagtac aaagacttta actcacgtaa ttaaatccgt taaagatcaa caaaacactt
      481 ttatttttac cgagtttaac ccagcaaaaa cgaagtattt catcctgaac aatggttcgg
      541 tggctttagc gggtcgagtg ttatccattg acgccacaga gaatggcagt gttattcata
      601 tttcactggt caacttatta agtacaccta tctcaaatat tggttttaat gcgacatggg
      661 gtggtgaaaa acctgtcgat gccaaagagt ttgcccggtg gcaacaatta cttttcaaca
      721 catcaatgaa atccacattg aaattattac caggtcaatg gcaagacatt aatttgaccc
      781 tgaagggtgt atcgcccaat aacctgggat atctgaagtt agccatcaac atggaaaata
      841 ttcagttcga caatcttccc tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc
      901 agttagtacg cttataacaa ggaatagcga gcatcattaa tgaacaaaat atttaaagtt
      961 atctggaacc ctgcgacagg gaattatact gttaccagcg aaacggcaaa aagccgtggc
     1021 aagaaatctg ggcgcagtaa gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg
     1081 tcgtttgggg cattggcgaa tgccgggaat gacaacggtc agggtgttga ttacggtagt
     1141 ggatcagctg gcgacggctg ggttgctata ggcaaagggg cgaaagcaaa tacttttatg
     1201 aacaccagtg gttccagtac tgctgtgggt tatgacgcta tagctgaagg ccaatatagc
     1261 tctgccatcg ggtcaaaaac ccatgcgatt ggtggtgcat caatggcctt tggggttagt
     1321 gcaatatcag aaggcgatag aagtatagca ctgggtgcct cttcgtattc attgggccaa
     1381 tactcaatgg ccctcggccg ttattcaaaa gcattgggta aattgtctat tgctatgggg
     1441 gactcttcca aagcggaagg agcaaacgcc attgccctgg gaaatgccac taaagctact
     1501 gagattatga gtattgctct tggcgacacc gccaatgcgt caaaagcgta ttcaatggcg
     1561 ctgggagcaa gtagcgtcgc atctgaagaa aacgctattg cgataggtgc tgagaccgaa
     1621 gccgctgaaa atgcaactgc tattggcaat aatgcgaagg caaaagggac taatagcatg
     1681 gcaatggggt tcggaagcct tgccgataaa gtcaatacta tcgcattagg aaatggcagc
     1741 caggctctgg cagataatgc aatcgccata ggccagggca acaaagctga tggcgtggat
     1801 gccatcgctc tgggtaatgg tagccagtcg agaggcttaa acaccattgc cttaggcaca
     1861 gccagtaatg caactggtga taagagtctt gcgcttggta gtaatagcag tgccaacggt
     1921 attaactctg tcgcgctggg cgcagattcc attgcggatt tagacaatac cgtctctgtc
     1981 ggcaatagtt cattaaaacg caagatcgtt aatgtgaaaa atggcgcgat caagtctgac
     2041 agttacgatg ccattaatgg ttcacagctt tatgccatta gcgactcggt agcaaaaagg
     2101 cttggaggag gggctgcagt agatgttgat gacggtactg ttacagcacc aacctacaat
     2161 ttaaaaaatg gtagcaaaaa taacgtaggg gctgcgctcg ctgtacttga tgaaaacacc
     2221 ctgcaatggg accaaaccaa aggcaaatac agcgctgctc atggtactag tagcccaact
     2281 gccagcgtaa tcaccgatgt tgcggatggc acgatttcag cctccagtaa ggatgcggtt
     2341 aacggttccc aactgaaagc taccaatgac gatgtcgaag ccaacaccgc caatatcgct
     2401 actaatacca gcaacattgc cacgaatacg gcaaatattg ccaccaatac caccaatatc
     2461 accaacctga cggattccgt tggtgacctt caggctgatg ccctgctctg gaacgaaact
     2521 aaaaaggcat tcagtgcagc tcacggccag gataccacca gcaaaatcac caacgttaaa
     2581 gatgccgacc tgacggctga cagcactgat gctgttaacg gctctcagct gaaaaccacc
     2641 aacgatgctg tggcgacgaa taccaccaat atcgccaata acacttccaa tattgccact
     2701 aacaccacca acatctctaa cctgactgag acggtgacta atcttggtga ggatgcgctg
     2761 aaatgggata aggacaatgg tgtattcacg gcagctcatg gcaccgagac caccagcaaa
     2821 atcaccaacg ttaaagatgg cgacctgacg actggcagca ccgatgccgt taacggctct
     2881 cagctgaaaa ccaccaacga tgccgtggcg acgaatacca ccaatatcgc cactaacacc
     2941 accaacatct ctaatctgac tgagacggtg actaatcttg gtgaggatgc gctgaaatgg
     3001 gataaggaca atggtgtctt cactgcagct catggcaaca ataccgccag caaaatcacc
     3061 aatatcctgg acggcacagt cactgcaacc agttccgatg ccattaacgg tagccagctt
     3121 tatgacttaa gcagcaatat cgccacctac ttcggcggca atgcttctgt gaatactgac
     3181 ggtgtgttta ccggtccaac ctacaaaatc ggtgaaacaa attattataa cgtcggcgat
     3241 gcactggctg cgattaactc ctcatttagc acgtctctcg gcgatgctct gctttgggat
     3301 gccaccgcag gtaaattcag tgccaaacac ggtactaatg gtgacgcaag cgtgatcact
     3361 gatgtcgcag atggtgaaat ttcagactcc agttctgacg cagtaaacgg ctcacaactc
     3421 cacggcgtga gcagttatgt tgttgatgcg ctggggggtg gtgccgaagt caatgcagac
     3481 ggcaccatca ctgcgccgac gtacaccatt gctaatgctg attacgataa tgtcggtgat
     3541 gccctgaatg ctatcgatac cactcttgac gacgctctgc tctgggatgc ggacgccggt
     3601 gaaaatggtg catttagcgc cgctcacgga aaagataaaa ctgccagtgt aatcactaac
     3661 gtcgctaacg gtgcaatctc tgctgccagc agcgacgcga ttaacggctc acaactctat
     3721 accaccaata agtacatcgc tgatgcgctg ggtggtgacg cagaagtcaa cgctgacggc
     3781 accatcaccg caccgactta caccattgcg aacgccgagt acaacaacgt cggtgacgcc
     3841 ctggatgcgc ttgatgataa cgccctgctg tgggatgaga ctgccaatgg cggtgctgga
     3901 gcctacaatg ccagccatga cggtaaagcc agcatcatca ctaatgtcgc taatggcagt
     3961 attagtgagg acagtaccga tgcagtgaac ggttctcagt tgaatgcgac gaatatgatg
     4021 attgagcaga acacccaaat tatcaatcag ctcgctggta acaccgacgc aacctatatc
     4081 caagaaaacg gtgcgggtat taactatgtg cgtactaacg acgacggctt agcgttcaac
     4141 gacgccagcg cacagggtgt tggcgctaca gctataggtt ataactctgt cgccaaaggc
     4201 gatagcagcg tagctattgg tcagggcagc tacagcgacg ttgatacggg tatcgccctg
     4261 ggtagcagct ctgtttccag ccgagtgatt gccaaaggct cccgtgacac cagcataacg
     4321 gaaaatggcg ttgttattgg ttacgacacc acggatggcg aactgctcgg tgcattgtct
     4381 atcggtgatg acggtaaata tcgtcaaatc atcaacgtag ccgatggttc cgaagcccat
     4441 gacgccgtta cggttcgtca attgcagaat gcgattggtg cggtcgcaac cacgccgact
     4501 aaatacttcc acgctaattc aacggaagaa gattcactgg cagtgggaac tgactcgctg
     4561 gcaatgggtg cgaaaaccat cgtgaatggc gataaaggta ttggtatcgg ttatggtgcc
     4621 tacgtggacg cgaatgcact taacggcatt gccattggta gcaatgcgca agtcattcat
     4681 gtcaacagta ttgcgatagg taatggttct acgaccactc gtggcgctca aaccaattat
     4741 accgcctaca acatggacgc accgcagaac tctgtcggtg aattctcagt cggtagtgcg
     4801 gatggtcaac gtcagatcac taacgtcgca gcaggttcgg ctgataccga tgcggtcaac
     4861 gtgggtcagt tgaaagtaac ggatgcgcag gtttcccaga atacccagag cattactaac
     4921 ctggataatc gggtaacgaa tcttgattca cgcgtcacca atatcgaaaa cggtattggc
     4981 gatatcgtca ccaccggtag caccaagtac ttcaagacca ataccgatgg tgtagatgcc
     5041 agcgcgcagg gtaaagatag cgtcgcgatt ggttccggct ccattgctgc cgctgacaac
     5101 agcgtcgctc tgggtacagg gtctgtggca accgaagaaa atacgatctc tgtaggttcc
     5161 tctactaacc aacgtcgtat caccaacgta gctgcaggta aaaatgctac cgatgctgtt
     5221 aacgtggcac agttgaagtc ttccgaagct ggcggtgtac gttacgacac caaagctgat
     5281 ggttctatcg actatagcaa tatcaccctc ggtggcggca acggcggtac gactcgtatc
     5341 agcaacgtct ccgctggcgt caacaacaac gacgtggtga attacgcgca gttgaagcaa
     5401 agcgtgcagg aaacgaagca atacaccgat cagcgaatgg ttgagatgga taacaaactg
     5461 tctaaaactg aaagcaagtt gagcggtggt atcgcttctg caatggcaat gaccggtctg
     5521 ccgcaggctt acactccagg tgccagcatg gcctctattg gtggcggtac ttacaacggt
     5581 gaatcggcag ttgctttagg tgtatcgatg gtgagcgcca atggtcgttg ggtctacaaa
     5641 ttacaaggta gtaccaatag ccagggtgaa tactccgccg cactcggtgc cggtattcag
     5701 tggtaatgat ccattaagtt agtgtgacta agggcaaacg ccaaattgcc tgatgcgtta
     5761 cgcgcatcag gcaagataag gcgctccggg tcaacaatct aagggcaatt ctctgatgag
     5821 gattgccctt ttctttacca gacatctccc cccacaagaa ttggccctac caattcttcg
     5881 cttatctgac ctctggttca caattcccca attaaaactc acatcaatat tgccaataca
     5941 taacatttag ttaaccattc attgtcatta tccctacaca acacaattgg cagtgccact
     6001 tttacacaac gtgtgacaag gagatgagca acagactcat tacacgatgt gcgtggactc
     6061 caggagacct gcaatgaatc tctggcaaca aaactacgat cccgccggga atatctggct
     6121 ttccagtttg atagcatcgc ttcccatcct gtttttcttc tttgcgctga ttaagctcaa
     6181 actgaaagga tacgtcgccg cctcatggac ggtagcaatc gcccttgccg tggctttgct
     6241 gttctataaa atgccggtcg ctaacgcgct ggcctcggtg gtttatggct tcttctatgg
     6301 tttgtggccc atcgcgtgga tcattattgc agcagtgttc gtctataaga tctcggtgaa
     6361 aaccgggcag tttgacatca ttcgctcgtc tattctttcg ataacccctg accagcgtct
     6421 gcaaatgctg atcgtcggtt tctgtttcgg cgctttcctt gaaggagccg caggctttgg
     6481 cgcaccggta gcaattaccg ccgcattgct ggtcggcctg ggttttaaac cgctgtacgc
     6541 cgccggactg tgcctgattg ttaacaccgc gccagtggca tttggcgcga tgggcattcc
     6601 aatcctggtt gccggacaag taacaggcat cgacagcttt gagattggcc agatggtggg
     6661 gcggcagcta ccgtttatga ccattatcgt gctgttctgg atcatggcga ttatggacgg
     6721 ctggcgcggt atcaaggaga cgtggcctgc ggtcgtggtt gcgggcggct cgtttgccat
     6781 cgctcagtac cttagctcta acttcattgg gccggagctg ccggacatta tctcttcgct
     6841 ggtatcactg ctctgcctga cgctgttcct caaacgctgg cagccagtgc gcgtattccg
     6901 ttttggtgat ttgggggcgt cacaggttga tatgacgcta gcccacaccg gttacactgc
     6961 gggtcaggtg ttacgtgcct ggacaccgtt cctgttcctg acagctaccg taacactgtg
     7021 gagtatcccg ccgtttaaag ccctgttcgc atcgggtggc gcgctgtatg agtgggtgat
     7081 caacattccg gtgccgtacc tcgataaact ggttgcccgt atgccgccag tggtcagcga
     7141 ggctacagcc tatgccgccg tgtttaagtt tgactggttc tctgccaccg gcaccgccat
     7201 tctgtttgct gcactgctct cgattgtctg gctgaagatg aaaccgtctg acgctatcag
     7261 caccttcggc agcacgctga aagaactggc tctacccatc tactccatcg gcatggtgct
     7321 ggcgttcgcc tttatctcga actattccgg actgtcatca acactggcac tggcactggc
     7381 gcacaccggt catgcattca ccttcttctc gccgttcctc ggctggctgg gggtattcct
     7441 gaccgggtcg gatacctcat ctaacgccct gttcgccgcg ctgcaagcca ccgcagcaca
     7501 acaaattggc gtctctgatc tgttgctggt tgccgccaat accaccggtg gcgtcaccgg
     7561 taagatgatc tccccgcaat ctatcgctat cgcctgtgcg gcggtaggcc tggtgggcaa
     7621 agagtctgat ttgttccgct ttactgtcaa acacagcctg atcttcacct gtatggtggg
     7681 cgtgatcacc acgcttcagg cttatgtctt aacgtggatg attccttaat gattgtttta
     7741 cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg cgctgattga tgaaaaaaac
     7801 ctggaagcgg gcatgaagtt gcccgctgag cgccaactgg cgatgcaact cggcgtatca
     7861 cgtaattcac tacgcgaggc gctggcaaaa ctggtgagcg aaggcgtgct gctcagtcga
     7921 cgcggcggcg ggacgtttat tcgctggcgt catgacacat ggtcggagca aaacatcgtc
     7981 cagccgctaa aaacgctgat ggccgatgat ccggattaca gtttcgatat tctggaagcc
     8041 cgctacgcca ttgaagccag caccgcatgg catgcggcaa tgcgcgccac acctggcgac
     8101 aaagaaaaga ttcagctttg ctttgaagca acgctaagtg aagacccgga tatcgcctca
     8161 caagcggacg ttcgttttca tctggcgatt gccgaagcct cacataacat cgtgctgctg
     8221 caaaccatac gcggtttctt cgatgtcctg caatcctcag tgaagcatag ccgtcagcgg
     8281 atgtatctgg tgccaccggt tttttcacaa ctgaccgaac aacatcaggc tgtcattgac
     8341 gccatttttg ctggtgatgc cgacggggcg cgtaaagcaa tgatggcgca ccttagtttt
     8401 gttcacacca ccatgaaacg attcgatgaa gatcaggctc gccacgcacg gattacccgc
     8461 ctgcccggtg agcataatga gcattcgagg gagaaaaacg catgattatt tccgcagcca
     8521 gcgattatcg cgccgcagcg caacgcattc tgccgccgtt cctgttccac tatatggatg
     8581 gtggtgcata ttctgaatac acgctgcgcc gcaacgtgga agatttgtca gaagtggcac
     8641 tgcgccagcg tattctgaaa aacatgtctg acttaagcct ggaaacgacg ctgtttaatg
     8701 agaaattgtc gatgccggta gcactggcac cggtgggttt gtgtggcatg tatgcgcgtc
     8761 gtggcgaagt tcaggcagcc aaagcggcgg acgcgcatgg cattccgttt actctctcaa
     8821 cggtttccgt ttgcccgatt gaagaagtcg cgccagccat caagcgccca atgtggttcc
     8881 agctttatgt actgcgcgat cgcggcttta tgcgtaacgc gctggagcga gcaaaagcag
     8941 cgggttgttc gacgctggtt ttcaccgtgg atatgccgac accgggagcg cgttatcgtg
     9001 atgcgcattc tgggatgagc ggcccaaatg cggcaatgcg ccgctacttg caagcggtga
     9061 cgcatccgca atgggcgtgg gatgtgggcc tgaacggtcg tccgcatgat ttaggtaata
     9121 tttcggctta cctcggcaaa ccgaccggac tggaagatta catcggctgg ctggggaata
     9181 acttcgatcc gtccatctca tggaaagacc ttgagtggat ccgcgatttc tgggatggcc
     9241 cgatggtgat caaagggatc ctcgatccgg aagatgcgcg cgatgcagta cgttttggtg
     9301 ctgatgggat tgtagtttct aaccacggtg gccgccagct ggacggtgta ctctcttccg
     9361 ctcgtgcgtt gcccgctatt gcagatgcgg tgaaaggtga tatcgccatt ctggcggata
     9421 gcggaattcg taacgggctt gatgtcgtgc gtatgattgc gctcggtgcc gacaccgtac
     9481 tgctgggtcg tgctttcctg tatgcgctgg caacagcggg ccaggcgggt gtagctaacc
     9541 tgctaaatct gatcgagaaa gagatgaaag tggcaatgac gctgactggc gcgaaatcga
     9601 tcagtgaaat tacgcaagat tcgctggtgc aggggctggg taaagagttg cctacggcac
     9661 tggctccgat ggcgaaaggg aatgcggcat agtcgtttga gccccctcac cctaaccctc
     9721 tccctcaggg agtggggacc gttcggcgct gtatgtactc cctcactctg aaacgacacc
     9781 gcactctttt tttctccctc gcccctccgg ggtgagggga aaaggccgca ctgctcacca
     9841 attttctgct atcctgcccc cgcactaagg gggcagtatg ctaaacatcg tactttacga
     9901 accagaaatt ccgccaaata ctggcaatat catccgtctt tgcgctaata ccggctttcg
     9961 tctgcatatc atcgaaccga tgggatttgc ctgggacgat aagcgcctgc gccgcgcggg
    10021 gctggactat cacgagttta ccgccgttac gcgtcatcat gactttcgcg cgttcctcga
    10081 agcagaaaat ccccagcgcc tgtttgccct caccacgaaa ggtacgcctg ctcacagcgc
    10141 cgtaaactat caggatggcg attatctgat gttcggcccg gaaacacgcg gcctgccagc
    10201 gagcattctt gatgccctgc ccgctgaaca aaaaattcgc attccgatgg tgccggacag
    10261 ccgcagcatg aacctgtcca atgcggtgtc agttgtggtg tatgaagcct ggcggcagct
    10321 gggatatccg ggagcgttgt tgagagatta gttactgtat gccggataga tgattacatc
    10381 gcatccggca cgatcacagg acattagatc ccatccccat actcaaatgt atggttaata
    10441 ccgttgaaat gctggtccat atccattgat ggcttatcgc tgtctggttt accgacaata
    10501 cgagccggaa cgccagcggc ggttgtatgc ggcggcaccg gttgcagcac cacggaacct
    10561 gcgccaatct tcgcgccgcg accaacttca atattgccga ggattttcgc gcccgcgcca
    10621 atcatcacac cttcacgaat tttcgggtga cggtcaccac cagatttacc cgtaccgcca
    10681 agcgtcacag attgcagaat cgatacgtcg ttttcaatta ccgccgtttc acccacaacg
    10741 atgcccgtgg cgtggtcgag cataatgcca cggccaattt ttgctgccgg gtgaatatcg
    10801 acctggaacg tcacagaaac ctggttttgc agaaagattg ccaatgcgcg acgcccctga
    10861 ttccacaacc agtgaccgat gcgataggcc tgcaaggcat gaaaaccctt caggtataac
    10921 aacggggttg agtatttatc gactgccggg tcgcgggtac gcaccgcctg aatatcacag
    10981 gccgcagagg cgatcatttc cgggtcagcg gcgtaggctt cttccaccac ttcacggata
    11041 gcaatagcag gcataattgg cgatgacagc ttgttcgcca gcatgtagct cagtgcacta
    11101 ccaaggtttt cgtgcttgag tagcgtcgcg tggtaaaaac tggccagcat tggctcacag
    11161 tccgccagcg ttctggcttc ggctttaata ttgttccaga caatttccag ttcttcacac
    11221 gacattgctt actccacacg atgagataat gaccggcccg ttctgcgcgg gccgggtcat
    11281 agcggtaaca aaggttccct ggggttagtg gctgctgcgc tcgtccttgc gtgcacgacc
    11341 taataacgtc aatgctgcct cgcgcgcgtt ttttccgcaa tataatactt gataaatttc
    11401 ctcggttatt ggcatttcta cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt
    11461 gcggtagcct tccaccacct gaccaatctt ctcctgcgcg ctttgtacat ccatgccctg
    11521 accgagcatc atgccaaaac ggcggttacg cgactggttg tcggtacaag taagcacaag
    11581 atcgcccagc cccgccatgc ccataaaggt ggcaggatcg gcacccagcg ccgcgccaag
    11641 acgcgacatt tcagccagcc cacgggtgat cagtgccgta cgcgcattcg caccaaaacc
    11701 gataccgtcg gacatccccg cgccaatggc aatgacgttt ttcaccgcac cgccaagctg
    11761 cacgccaatg aaatccggat tgctgtaaac gcggaaactt ttgccgcagt gcagcagctg
    11821 ctggagatca tcggcaaagg tctgatcggt cgaggccagc gaaatagctg tcggtaaacc
    11881 tgccgccagt tctttcgcaa acgttgggcc agagataacc gccagcggaa tttgatcgcc
    11941 taaggcctca cgcgccacgt cctgtaacag acgtccggtt tccgcttcca gccctttggt
    12001 cgcccacacc agacgcgcat caggacgcat cagcggttta atctggcgca gcacttcacc
    12061 aaagacatgg ctgggtacga cgacgagaat attacggctg gctgccagcg cagtggcgag
    12121 atcgctttca agatggagcg tatcgggaaa aggcacatcg gggagaaacg cggcgttaca
    12181 gcggtcgcgt tcaagcgttg cgatatgttc agggtcatgg ccccagagga caacctcgtg
    12241 gccatttctt gccagggtga tggcaagagc ggtgccgtac gagccggcac cgatcacagt
    12301 cattgaagca ttacgttggt tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc
    12361 tgctgctgca aatagttcat gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc
    12421 gggaatgtac cgcgggatac catgctggtg atgcactcac gagcatacgg gaacagaatg
    12481 ttcgggcagt atgcgcccag gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag
    12541 aaaataccgc cctgctgaac ttcacacagg aacgcggttt cttcgcccag ggaggccgtt
    12601 acggtaacac gcagtaccac ttcgtatacg tcatctgcca gttgggaaga tgccgtatcc
    12661 agatcaagtt taacttctgg ttgccaatct ttctggaaaa cgtgcggcgc gttcggcgct
    12721 tcgaaagaga tatccttggt ataaatacgt tggatctgga aagtcatttc agtgttgttt
    12781 tgttctgaca tgtgtagaaa acccttaagt gttgtcctta aatactgcgc aatgtaatgc
    12841 caacgttcac acgttatttc agcaggggat ccagtccacc acgtgcatcc aatgcataca
    12901 agtcatcaca gccgccaatg tgctgtgcgt caataaaaat ctggggaacc gtggtgcgac
    12961 cgctgcgttt gatcatctct tcacgcttgg cggcattgcc atcgatcggc aactcctgaa
    13021 aactcacgcc cttgctgctc agcagtgctt ttgcacgatg gcaatacggg caggtttctt
    13081 tggtatagat ttcaacattg gccataattt agctcctgtt tttttaccct gaaatttcat
    13141 gttgcagggc ggctgcaaaa tggagtgtcc ccagaagctt ataacaataa gtcgctgggg
    13201 gcctaattgc agctaacggc ctgcatcatg aaagacgaca ggtaaattat ttgccgcgca
    13261 ccaaaggcaa gttttcgcca gcccagccag cgacgccttc tttcagcacg aatacttgcg
    13321 caaaaccagc tttcgtcagt gcgtttgcag gctcctggca ctgcatgcca gaaccgtcta
    13381 ccacgataac cggtttgtct ttgtgcttct caagctcacc aacattgttg gctttgattt
    13441 cgctcggcaa cagattgata gaacctgcga tatggccttt acggaagtca tcacgctgac
    13501 gtaaatccac aaccacagcg tcttctttgt tgatcagacg cgtagcttca ccacgagtaa
    13561 tcaccttcac tttcgaggtc aggctcttaa acgtagtcac aagaaccgcc accagtaacg
    13621 cgatccaggc gatactcagt atgggatgac ggccaacaaa ttgcataatt tcttgcatgg
    13681 ggggtaacaa ctcccgacgt agtgattaaa aaaaccagga aaggagtata cctgcgcggt
    13741 gcggcaaata cagccagcgc gttaactgga atgcaatttt gcggggcacg acgaaaaaaa
    13801 agcgcaaatc tggcatcggc ttgccgcgag cggtcgtata ttttgatctt cagaggctat
    13861 tttatcgatt cagctgtagt aaaattacgc aaattttgac tcttgagtat gaggttgtcg
    13921 caatgtcggt ttctaaaaaa cctatggtac tggtgattct ggatggctat ggctatcgtg
    13981 aagaacagca ggataacgcc atttttagtg ctaaaacccc ggtaatggat gcactgtggg
    14041 ccaatcgtcc gcataccctg atcgacgctt ccggtctgga agtcggtctg cctgaccgtc
    14101 agatgggtaa ctccgaagta ggccacgtta acctgggtgc cggccgcatc gtgtatcagg
    14161 acctgactcg tctggacgtt gaaatcaaag atcgcgcttt ctttgctaat ccagtgctga
    14221 ctggcgcggt agataaagcg aaaaacgcag gtaaagcagt acacattatg ggtctgctct
    14281 ccgcaggcgg cgtacacagc cacgaagatc acatcatggc gatggtagaa ctggcagctg
    14341 aacgcggcgc agaaaaaatc tacctgcacg cattccttga cggtcgcgac accccgccgc
    14401 gcagtgctga atcctcgctg aaaaaatttg aagagaaatt tgctgcgctg ggtaaaggcc
    14461 gcgtagcgtc catcattggt cgttactacg cgatggaccg cgataaccgt tgggatcgcg
    14521 ttgaaaaagc ctatgacctg ctgactctgg cgcagggtga gttccaggcc gatactgccg
    14581 ttgctggttt gcaggcagct tatgctcgcg acgaaaacga cgagttcgtg aaagcgaccg
    14641 ttattcgtgc agaaggtcag ccagatgcgg caatggaaga cggcgatgcg ctgattttca
    14701 tgaacttccg tgctgaccgc gcgcgcgaaa tcactcgtgc attcgtaaac gcagatttcg
    14761 acggcttcgc gcgtaagaaa gtggttaacg tcgatttcgt gatgctgacc gaatacgccg
    14821 ctgacatcaa aactgcggtt gcttacccac ccgcttccct ggttaacacc ttcggcgagt
    14881 ggatggcgaa aaacgacaaa actcagttgc gtatttccga aaccgaaaaa tatgcccacg
    14941 ttactttctt cttcaacggt ggcgtagaag agtcgttcaa aggcgaagat cgcattctga
    15001 tcaactcacc gaaagtggct acctacgatc tgcaaccgga aatgagctcc gcagagctga
    15061 ccgaaaaact ggttgcggcc atcaagagcg gcaaatacga caccattatc tgtaactatc
    15121 cgaacggcga catggtaggt cacaccgggg tgatggaagc ggcggttaaa gcggttgaag
    15181 cactggatca ctgcgtggaa gaagtcgcga aagcggttga gtccgtgggt ggacaactgc
    15241 tgatcaccgc tgaccacggt aacgctgaac agatgcgcga tccggcaacg ggtcaggcac
    15301 acacggcaca caccaacctg ccagttccgc tgatttacgt tggtgataag aacgtgaaag
    15361 cggttgcagg cggcaaactt tctgacatcg cgccgaccat gttgtcgctg atgggtatgg
    15421 aaatcccgca agagatgact ggtaagccgc tgttcatcgt ggaataatcc ctccccatga
    15481 ggggaaaggc gattaatacc atgacacggg ccgtgaaacc gcgcaggttt gcaatcaggc
    15541 ccatcatcta cgccagcgtt cttagcgctg gcgtattgtt gtgcgccttt tccgcccacg
    15601 cggatgagcg tgaccaactc aaatctattc aggccgatat cgccgcaaaa gagcgcgcgg
    15661 tacgccaaaa gcaacaacaa cgcgcaagcc tgctcgcaca attgaaaaag caggaagaag
    15721 cgatctctga agccacccgt aagctgcgcg aaacgcaaaa cacgctcaat caactcaata
    15781 aacagattga tgagatgaac gcgtcgattg ccaaactgga gcagcaaaaa gccgcccagg
    15841 agcgcagcct cgccgcacaa ctggatgccg cattccgtca gggcgagcat accggtattc
    15901 agctgattct cagcggtgaa gaaagccagc gtggacagcg tttacaggct tatttcggct
    15961 atctcaacca ggcgcgacaa gaaaccattg cccagttgaa gcaaacgcgt gaagaagtcg
    16021 ccatgcagcg tgctgaactg gaagagaaac agagcgagca acaaacgctg ttatatgagc
    16081 agcgcgccca acaggcgaaa ctgactcagg cgctgaacga gcgtaaaaag acgctggcag
    16141 ggctggagtc ttccatccag caaggtcagc aacagttgag cgagctgcgc gccaacgaat
    16201 cccgtctgcg taacagcatt gcccgtgcgg aagccgcggc gaaagcgcgt gcagaacgag
    16261 aagcacgtga ggcccaggcg gttcgcgacc gccagaaaga agcgacgcgc aaaggcacca
    16321 cctacaaacc gaccgaaagc gaaaaatcgc tgatgtcccg aactggtggc ctgggggcgc
    16381 cgcgtggtca ggcattctgg ccggttcgcg ggccgacgct gcatcgctat ggtgaacagc
    16441 tacagggcga actacgctgg aaaggaatgg ttatcggtgc ctctgaaggt actgaagtta
    16501 aagcgattgc cgatggtcgg gtgattctgg ctgactggct gcaaggttac ggtctggtgg
    16561 tggtggttga gcacggtaaa ggcgacatga gtctttacgg ctataatcag agcgcactgg
    16621 tgagcgttgg ttcgcaggtt cgcgcgggcc agccaattgc actggtgggc agcagtggcg
    16681 gtcagggtcg gccttcactc tatttcgaaa ttcgccgcca aggtcaggcg gtcaatccac
    16741 agccgtggtt gggaagataa gttttgtttc catttcgtcg taacgttctt gcatttgccg
    16801 ctctgttggc gctctcctcc cccgtacttg ctggcaaact tgccatcgtc attgatgatt
    16861 ttgggtatcg cccgcacaac gaaaaccagg tgctggcgat gccctccgct atctccgtcg
    16921 ctgtattacc cgattcaccg cacgccagag agatggcgac caaagcgcat aacagcgggc
    16981 acgaagtgtt gattcatctc ccgatggcac cactgagtaa acagccgctg gagaaaaata
    17041 cgctgcgccc ggagatgagc agcgacgaaa ttgagcgcat tattcgtagt gcggtcaata
    17101 acgtgcccta tgccgtgggg atcaacaacc acatgggtag caagatgacc tctaacctgt
    17161 ttggtatgca gaaagtgatg caggcactgg agcgttacaa tctttacttc ctcgacagcg
    17221 taaccatcgg taatacccag gcgatgcgcg ccgcgcaagg tactggcgtg aaggtgatca
    17281 aacggaaggt gttcctcgac gattcgcaga acgaagcgga catccgtgtg caatttaatc
    17341 gcgcaattga tctggcgcgt cgcaacggtt caaccattgc cattgggcat cctcaccctt
    17401 caacggtacg cgtgctacaa cagatggttt ataacctgcc gccagacatt acgctggtga
    17461 aagccagcag cttgctgaat gaaccgcagg ttgatacttc tacaccgccg aaaaacgctg
    17521 tgcctgatgc accgcgtaat ccgttccgtg gcgtgaagct gtgcaaaccg aagaaaccga
    17581 tagaacctgt ttatgctaat cgcttctttg aagtattaag cgaaagcatc agccagagca
    17641 cgctgatcgt ttacttccag catcagtggc aaggctgggg caaacagccc gaagcggcga
    17701 agtttaacgc tagcgcaaat taaaggcgct acgcgtaatg cgatggtgcg accgcgtttt
    17761 tttcgcgcca ttgccataag cggaaagacc acagtaacgc ctggtaaccg accttcacgc
    17821 tgcgcacatt ggtaatcagg cgcttataca taccggaagt aaatatctcg gcaatcatcc
    17881 gttggcgggt aagaatatcc ggctctttgc gcaccgcatg gcaaacacgc aatgcttcgt
    17941 aagttatttg ctgatgaaat tccggataaa tcataatttt gtcggcataa tttcgattta
    18001 atttctccag caggcgggta atcttaatat agtgacgttg ataattaagg tttttattcc
    18061 cttgtctatg caaccgactc actgacgtat tatgcagata atatttatat aatgattgct
    18121 cggtatatcg cgcacgcagc gcgttaaaca tgaattctgt tgtccagaca atatcctgat
    18181 gatgtaatcc ggcaataaat ttaatgttat ttttaacaat aacatcacgg cgataaaccc
    18241 ccatccagac aacgtgagtc caacggcgcg aagaaagccc catccgcagc cagtccgggc
    18301 cggttaatac accggttgag cgaaggcgat cgctggggat ggattgccag gtttctcccg
    18361 tttcacgaaa agaccagtca gcgttgcact gcgccacgtc gaggtcgtcc tctaacgcca
    18421 tggtcatcag cgtttcgtac atggtgggat agacctcatc gtcagcatcg acaaaagcga
    18481 catatttgcc cgtcgccacc tcgatcccac gattacgcgc taccgatgcg ccagcattcg
    18541 cctgatgcaa caaacgaacg tgcggatagt tttctgcgta atgctttgct atttcaacag
    18601 aattatccgt tgaaccatcg ttaataataa tgatttccag agcagtccag gtttgcgtaa
    18661 ttaaagattc catacaagtg cggaaatcat cgcccgcatt atataacgga ataataacac
    18721 taagtttatt ggtgctgttc atcataaatt accgacgacc tctgaagtta tcctgacgtt
    18781 ttacaggctt tgtattaaga aactattaag cctgtgagat attattttta caggtgagag
    18841 agtcacttca gggtagagag ataatgagaa cagctatata aaagatcagc gcaattacga
    18901 actctttatt acactgacag gattttacat atttaacaga ttatcctcag gcaaaagggc
    18961 tggaatacca gccctttttc gtgttaatcc cagctcagaa taactttccc ggactggccc
    19021 gaacacatag cgtcaaagcc cttctggaaa tcatcgatag agaaacgatg ggtaatgatc
    19081 ggcgagagat cgaggccaga ctgaatcagc gccgccatct tgtaccaggt ttcaaacatc
    19141 tcacgaccgt aaatgccttt aatgaacaag cctttaaaga tcactttggt ccagtcgata
    19201 gacatgtcag acggtggaat acccagcatc gcaatacgac cgccgtggtt catggtatcg
    19261 agcatggtac gaaacgctgg cggcgcgccg gacatttcca gaccgacatc aaagccttcg
    19321 gtcatgccca gttcagccat cacatcatta aggttttctt tcgcgacgtt aaccgcacgg
    19381 gtgataccca ttttgcgcgc cagctcaagg cggtattcgt taacatcggt gatcaccaca
    19441 ttgcgtgcac caacgtgttt cgccaccgcc gcagccataa tacctatcgg acctgcacca
    19501 gaaaccagca catcttcgcc caccagatcg aacgacagcg cggtatgcac ggcgttaccg
    19561 aagggatcaa aaatggaagc caggtcgtcg gagatattgt cggggatttt gaaagcgttg
    19621 aacgccggaa tcaccagata ttcggcgaag cagcccggac ggttaacacc gacaccaatg
    19681 gtgttgcggc acagatgggt acgaccgcca cgacagttgc gacagtgacc gcaggtgata
    19741 tggccttcgc cagaaacgcg atcgccgatt ttgaagcctt tcacttcctg accaatacct
    19801 accacttcac cgacatattc atggcctacg accatcggca ccgggatggt tttttgcgac
    19861 cactcatccc agttatagat gtgaacgtca gtcccgcaga tggctgtttt acggatttta
    19921 atcagcagat cgttatgccc gagttccggt acaggaacgt cggtcatcca gatgccctct
    19981 tccgctttca gtttggataa cgctttcatc tcacatcctc aggcgataac gcccagttgt
    20041 ttaccaatac gcgtgaatgc ttctactgca cgcgtaattt gctcaggggt atgcgccgca
    20101 gacatctggg tacgaatacg cgcctgacct ttcggaacga ccggatagaa gaaaccggtg
    20161 acgtaaatgc cctctttttg cagctcacgg gcaaatttct gcgccactac tgcatcgcca
    20221 agcatgaccg gaataatggc atgatcggct cccgccaggg taaagcccgc cgccgacatt
    20281 tgctcacgga actggcgcgc gttcgcccac agacggtcac gcagttcgct gcccgcttcg
    20341 accatctcca gtactttgat ggacgcggca acaatggccg gtgccagcga gttggagaac
    20401 aggtacggac gagaacgctg gcgcagccac tcaaccacct ctttgcgcgc cgcggtataa
    20461 ccaccagaag ccccgcccag cgctttacca agcgtaccgg tgataatatc gacccggccc
    20521 atcacatcgc agtattcatg ggaaccacga ccattttcac cgacaaaacc gaccgcgtgg
    20581 gagtcatcca ccatcaccag ggcatcgtat ttatctgcca gatcgcaaac gcctttcagg
    20641 ttggcaatca cgccgtccat tgagaacaca ccatcggtgg caatcagcac atgacgcgca
    20701 ccggcttcac gcgcttcttt cagacgtgct tccagctcct gcatatcgtt gttggcatag
    20761 cgatagcgtt tagctttgca cagacgcaca ccatcaataa tagacgcgtg gttcagtgcg
    20821 tcggagataa tggcgtcttc cgcacccagc agcgtttcaa acagtccacc gttagcatca
    20881 aagcaggaag agtagagaat cgcatcttcc atccccagga atgccgccag tttttgttca
    20941 agctctttat ggctgtcctg agtgccgcaa ataaaacgca ccgaagccat gccgaaaccg
    21001 tgagaatcca ttcccgcctt tgctgccgca atcagatccg gatgattcgc cagcccgaga
    21061 tagttgttgg cacaaaagtt aatgacgtgg cttccatcag ccacagtgat atctgcttgc
    21121 tgcgcagacg taataatgcg ctcttcttta aacaaccctt ccgcccgtgc ggtttccaga
    21181 tcgttggtta actgctgata aaattctcca cgcattgcga ttctccagac agggcaaatt
    21241 ccagcacata ttacccaaac gtataggtcg cgacgagatg acgcgttaac tcttctgcaa
    21301 aattcaggat aaatgacgtg taacttgatg tctctcaaca cctacagata gtagaaaaac
    21361 tgtacagttt tggctgggca taatgatgat tgattttact gcattgatat aaaaagaaaa
    21421 aatgatgcca aagcatcatt tttcctatct cagttggtat caaaacgcta cccactaatg
    21481 gagggaaacc tattttcttg aaaggaaaat tctcagcgcc tgcatttttg cccctaaagt
    21541 gttactcctg aagcagcgaa acaaatcgaa ccattttcct ctccccagat agtttaggtt
    21601 gaagagatcg gggcgtttgt aatagcacat gacaaaaatg ccctggtcat catccacaat
    21661 gttattattt agtgttattt tttgactttc cagaactaat ttatagaatt ctttccattt
    21721 atgctgtgaa ccaacaattg cgcccccaat aatatataca tgattgccaa tcatgaaatc
    21781 aaaaacctgt tgttgagatg tgacagtaag cccttttttt atagtgaaaa gatgcatctt
    21841 actttcatca aatgggaaat cccaaatttt caatcctcga gttacatttg gcttacggca
    21901 atagccaaaa tctatccacg caacaagagg cgttttcact aagcccatgt tgatagcttt
    21961 atttacaaaa taggccttaa ggttacatac caaaacatac tctggtgacc agtactctgg
    22021 gttttttaat tgtcgaggct caagtctatt tgtaaacgac tcatcttttt gaattttttc
    22081 gatacgactt ctgatatatc taaatttctt ttttatatcg ataacgataa ccgttgttgg
    22141 ttttccgttg cgaatcgctt caacccttgg tttcaaatcg ggagaagtga aaataatcat
    22201 ctcattttca agtgcggcca gacgctcaaa gtaactaaaa taaacatcaa ctgaacgagc
    22261 tagtttttca cggaaccctt tattagcagt ccaatcccct ctaccaatgt caaaatatgc
    22321 agt
//
LOCUS       DAZKIF010000083        21151 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZKIF010000083 DAZKIF010000000
VERSION     DAZKIF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.083"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            102..242
                     /locus_tag="ACHOTW_003045"
                     /pseudo
     CDS             102..242
                     /locus_tag="ACHOTW_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(267..581)
                     /gene="fliE"
                     /locus_tag="ACHOTW_003046"
     CDS             complement(267..581)
                     /gene="fliE"
                     /locus_tag="ACHOTW_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP9845107.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            796..2454
                     /gene="fliF"
                     /locus_tag="ACHOTW_003047"
     CDS             796..2454
                     /gene="fliF"
                     /locus_tag="ACHOTW_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP9845108.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSWRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2447..3442
                     /gene="fliG"
                     /locus_tag="ACHOTW_003048"
     CDS             2447..3442
                     /gene="fliG"
                     /locus_tag="ACHOTW_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP9845109.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3435..4121
                     /gene="fliH"
                     /locus_tag="ACHOTW_003049"
     CDS             3435..4121
                     /gene="fliH"
                     /locus_tag="ACHOTW_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP9845110.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4183..5556
                     /gene="fliI"
                     /locus_tag="ACHOTW_003050"
     CDS             4183..5556
                     /gene="fliI"
                     /locus_tag="ACHOTW_003050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP9845111.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5575..6018
                     /gene="fliJ"
                     /locus_tag="ACHOTW_003051"
     CDS             5575..6018
                     /gene="fliJ"
                     /locus_tag="ACHOTW_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP9845112.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6015..7142
                     /gene="fliK"
                     /locus_tag="ACHOTW_003052"
     CDS             6015..7142
                     /gene="fliK"
                     /locus_tag="ACHOTW_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP9845113.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7247..7711
                     /gene="fliL"
                     /locus_tag="ACHOTW_003053"
     CDS             7247..7711
                     /gene="fliL"
                     /locus_tag="ACHOTW_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP9845114.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7716..8720
                     /gene="fliM"
                     /locus_tag="ACHOTW_003054"
     CDS             7716..8720
                     /gene="fliM"
                     /locus_tag="ACHOTW_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP9845115.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8717..9130
                     /gene="fliN"
                     /locus_tag="ACHOTW_003055"
     CDS             8717..9130
                     /gene="fliN"
                     /locus_tag="ACHOTW_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP9845116.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9133..9498
                     /gene="fliO"
                     /locus_tag="ACHOTW_003056"
     CDS             9133..9498
                     /gene="fliO"
                     /locus_tag="ACHOTW_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP9845117.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9498..10235
                     /gene="fliP"
                     /locus_tag="ACHOTW_003057"
     CDS             9498..10235
                     /gene="fliP"
                     /locus_tag="ACHOTW_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP9845118.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10245..10514
                     /gene="fliQ"
                     /locus_tag="ACHOTW_003058"
     CDS             10245..10514
                     /gene="fliQ"
                     /locus_tag="ACHOTW_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP9845119.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10522..11307
                     /gene="fliR"
                     /locus_tag="ACHOTW_003059"
     CDS             10522..11307
                     /gene="fliR"
                     /locus_tag="ACHOTW_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP9845120.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            11597..12220
                     /gene="rcsA"
                     /locus_tag="ACHOTW_003060"
     CDS             11597..12220
                     /gene="rcsA"
                     /locus_tag="ACHOTW_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP9845121.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12264..12452)
                     /gene="dsrB"
                     /locus_tag="ACHOTW_003061"
     CDS             complement(12264..12452)
                     /gene="dsrB"
                     /locus_tag="ACHOTW_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP9845122.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12615..12842
                     /gene="yodD"
                     /locus_tag="ACHOTW_003062"
     CDS             12615..12842
                     /gene="yodD"
                     /locus_tag="ACHOTW_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP9845123.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13140..13955
                     /gene="yedP"
                     /locus_tag="ACHOTW_003063"
     CDS             13140..13955
                     /gene="yedP"
                     /locus_tag="ACHOTW_003063"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP9845124.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(13952..15646)
                     /gene="dgcQ"
                     /locus_tag="ACHOTW_003064"
     CDS             complement(13952..15646)
                     /gene="dgcQ"
                     /locus_tag="ACHOTW_003064"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP9845125.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(15817..15999)
                     /gene="yodC"
                     /locus_tag="ACHOTW_003065"
     CDS             complement(15817..15999)
                     /gene="yodC"
                     /locus_tag="ACHOTW_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP9845126.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(16078..16995)
                     /gene="yedI"
                     /locus_tag="ACHOTW_003066"
     CDS             complement(16078..16995)
                     /gene="yedI"
                     /locus_tag="ACHOTW_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP9845127.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            17168..18088
                     /gene="yedA"
                     /locus_tag="ACHOTW_003067"
     CDS             17168..18088
                     /gene="yedA"
                     /locus_tag="ACHOTW_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP9845128.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18077..18547)
                     /gene="vsr"
                     /locus_tag="ACHOTW_003068"
     CDS             complement(18077..18547)
                     /gene="vsr"
                     /locus_tag="ACHOTW_003068"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP9845129.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18528..19946)
                     /gene="dcm"
                     /locus_tag="ACHOTW_003069"
     CDS             complement(18528..19946)
                     /gene="dcm"
                     /locus_tag="ACHOTW_003069"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP9845130.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(20013..20708)
                     /gene="yedJ"
                     /locus_tag="ACHOTW_003070"
     CDS             complement(20013..20708)
                     /gene="yedJ"
                     /locus_tag="ACHOTW_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP9845131.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(20748..21028)
                     /gene="drpB"
                     /locus_tag="ACHOTW_003071"
                     /pseudo
     CDS             complement(20748..21028)
                     /gene="drpB"
                     /locus_tag="ACHOTW_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
BASE COUNT         5092 a         5624 c         5464 g         4971 t
ORIGIN      
        1 tccatagccc ggaacatgaa taaaaacatc aacaaagata aaattatcca gaggttactg
       61 ttgcgtctgt ggtcgtaaca acatccgacg tggtgatgtg tcaaatgaga cactctgatg
      121 ttcagggggt ttatcgtgta tacggatcgt ggatggcaga aaactttcag gatcaggtat
      181 ccatttcaaa ccagataatg agtaagtttg ccccatccat gccccacgcg gtgagatcgg
      241 atgtcattaa caacaggtta cacaacctat acctgcatgc tcattacttc ctgatatgcc
      301 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
      361 tcggtcatca catcgtttaa cgccacgccg ggttcaccga gagtgaattt ttctgcctgc
      421 gtgcgggcgg ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca
      481 aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt
      541 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcatctc ctggataatt
      601 tctggtagca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
      661 acgggtttat tggcggatag aaaaaaacga aagcacaaat aatgggagcg tcaatttttc
      721 gagtttgctg acccgggagt gagtcttgtt ccactttgcc aataacgccg tccataatca
      781 gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa aatctcttga gtggcttaat
      841 cgcctgcgtg cgaatccgaa aattccattg attgttgccg gttccgcggc agtggcggtc
      901 atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc gcacattatt cagcaatctt
      961 tccgatcagg atggtggcgc aattgtcagc caactgacgc aaatgaatat tccttaccgc
     1021 ttcagcgaag ccagcggcgc tattgaagtt ccggcagata aagttcacga gctgcgcctg
     1081 cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact actcgatcag
     1141 gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt atcagcgggc gctggaaggc
     1201 gagctttctc gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt acatctggca
     1261 atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta
     1321 aatctgttac ccggccgcgc actcgatgaa gggcaaatta gcgccattgt gcatctggtt
     1381 tccagcgccg ttgctggtct gccgccggga aacgtcacgc tggtggatca gggcggacat
     1441 ctgttaaccc agtccaatac cagctggcgc gatcttaatg acgctcaatt gaaatatgcc
     1501 agcgatgtcg aaggccgtat tcagcggcgt attgaagcaa tcctgtcgcc tattgttggt
     1561 aacggtaata ttcacgccca ggtcacggcg caactggact tcgccagtaa agaacaaacg
     1621 gaagaacagt atcgccctaa tggtgatgaa tctcaggcgg cgcttcgttc acgccagctt
     1681 aatgagagcg agcaaagcgg ttccggttat ccgggcggcg taccgggggc gttgtcgaat
     1741 caaccggctc ctgcgaataa cgcgccaatc agcacgcctc cggcaaatca aaataaccgc
     1801 cagcagcagg cgagcaccac cagcaatagt gggccgcgta gcacacagcg gaatgaaacc
     1861 agtaactacg aagtcgatcg cactattcgt cataccaaaa tgaacgtggg cgatgtgcaa
     1921 cgtctgtcag tcgcggtagt ggtgaattac aaaaccttgc cagacggtaa accattgcct
     1981 ctcagcaacg aacagatgaa acaaattgaa gatctgaccc gcgaggcgat gggcttttct
     2041 gaaaaacgcg gtgactcgct caatgtcgtt aactcgccgt tcaatagtag tgacgaaagc
     2101 ggcggagaac tgccgttctg gcaacagcaa gcgtttatcg atcagttact ggctgccggt
     2161 cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc gtaaagcggt acgcccgcag
     2221 ctaacacgtc gcgctgaggc gatgaaagct gtacagcaac aggcgcaggc ccgcgaggaa
     2281 gtggaagatg cggtggaagt ccgcctgagc aaagacgaac aactccaaca acggcgcgct
     2341 aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat
     2401 ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac
     2461 aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaagt
     2521 gttcaagcac ctctcccagc gcgaggtgca aaccctgagc gctgcaatgg cgaacgtcac
     2581 gcagatctcc aacaagcagc taaccgatgt gctggcggag tttgagcaag aagctgaaca
     2641 gtttgccgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct
     2701 gggtgaggaa cgtgccgcca gcctgctgga agatattctc gaaactcgcg ataccgccag
     2761 cggtattgaa acgctcaact ttatggagcc gcagagcgcc gccgatctga ttcgtgatga
     2821 gcatccgcaa attatcgcca ccattctggt gcatctgaag cgcgcccagg ccgccgatat
     2881 tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg
     2941 cggcgtgcag ccagccgcgc tggcggaact gaccgaagta ctgaatggct tgctcgacgg
     3001 tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct
     3061 gatgaaaact cagcaggaag aagccgttat taccgccgtg cgtgaattcg acggcgagct
     3121 ggcgcagaaa atcatcgacg agatgttcct gttcgaaaat ctggtggatg tcgacgatcg
     3181 cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgttgatcg cgctgaaagg
     3241 ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat
     3301 tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg aaaacgaaca
     3361 gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact ggcgagatgg taattggcag
     3421 cggcgaggat acctatgtct gataatctgc cctggaaaac ctggacgccg gacgatctcg
     3481 cgccaccaca ggcagagttt gtgcccatgg tcgagtcgga agaaaccatc attgaagagg
     3541 ctgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccat gagcaaggtt
     3601 atcaggcggg tattgccgaa ggtcgccagc aaggtcataa gcagggctat caggaaggac
     3661 tggcccaggg gctggagcaa ggtctggcag aggcgaagtc tcaacaagcg ccaattcatg
     3721 cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcactt gatagtgtga
     3781 tagcgtcgcg cctgatgcag atggcgctgg aggcggcacg tcaggtcatc ggtcagacgc
     3841 caacggtgga taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt
     3901 tattcagcgg taaaccacag ctgcgcgtgc acccggatga tctgcaacgt gtggatgata
     3961 tgctcggcgc taccttaagt ttgcatggct ggcgcttgcg gggcgatccc accctccatc
     4021 ctggcggctg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct
     4081 ggcaagaact ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg
     4141 ctggcaagaa ctctgccgtc tggcagcacc aggagtggtg taatgaccac gcgcctgact
     4201 cgctggctaa ccacgctgga taactttgaa gccaaaatgg cgcagttgcc tgcggtacgt
     4261 cgctacgggc gattaacccg cgctaccggg ctggtgctgg aagccaccgg attacaattg
     4321 ccgctcggcg caacctgtgt cattgaacgc cagaacggca gcgaaacgca cgaagtagaa
     4381 agcgaagtcg ttggctttaa cggtcaacgg ctgtttttaa tgccgctgga ggaagtggaa
     4441 ggtgtcctgc ccggcgcgcg ggtttatgcc aaaaacattt cggctgaagg actgcaaagc
     4501 ggcaagcagt tgccgctcgg tccggcgtta ttaggtcgcg ttctggacgg cagcggtaaa
     4561 ccgctcgatg gcctgccctc ccccgatacg acggaaaccg gtgcgctgat taccccgcca
     4621 tttaacccgt tgcaacgtac accgattgaa catgtgctgg acaccggcgt gcgcccaatc
     4681 aatgccctgc ttaccgttgg gcgtgggcag cgtatggggc tgtttgccgg gtccggcgtt
     4741 ggtaaaagtg tgctgctggg gatgatggca cgttacaccc gcgccgatgt cattgtcgtg
     4801 ggtttgattg gtgaacgtgg gcgcgaagta aaagatttta ttgagaatat cctcggtgcc
     4861 gaagggcgtg cacgatcagt ggtgattgcc gctccggcgg atgtgtctcc gctcctgcga
     4921 atgcaaggtg ccgcctatgc cacgcgcatt gccgaagatt ttcgcgatcg tggtcagcat
     4981 gtgttgctga ttatggactc cctcacccgc tacgcgatgg cccagcgtga aattgcgctg
     5041 gcgattggcg aaccacccgc caccaaaggt tatccaccgt cggtgtttgc caaattaccg
     5101 gcactggtcg agcgtgccgg aaatggcatt agcggcggcg gctcgattac cgcgttttat
     5161 accgtgctta ctgaaggcga tgaccagcag gaccccattg cagactccgc gcgggcgatc
     5221 ctcgacggtc acattgtgct gtctcgccga ctggcggaag ccgggcacta tccggctatc
     5281 gatattgaag cgtcgatcag ccgcgcaatg acggcgttga tcagtgagca acattacgcg
     5341 cgagtgcgca ccttcaaaca gctgttgtcg agttttcagc gtaaccgcga tctggttagc
     5401 gtcggcgcgt atgccaaagg cagcgatccg atgctcgaca aagccatcgc cctgtggccg
     5461 cagctggagg gctatttgca acaaggcatt tttgaacgcg cggactggga agcgtctctc
     5521 caggggctgg agcgtatttt cccgacagtg tcataaccca ggagataacg gcagatggca
     5581 gaacatggcg cgctggcgac cctgaaagat ctggcagaaa aagaggtaga ggatgccgcg
     5641 cgcctgctgg gtgaaatgcg tcgcgggtgt cagcaggcgg aagaacagct caaaatgctg
     5701 atcgattatc agaatgaata tcgcaataac ctcaacagcg atatgagtgc cgggatgacc
     5761 agcaaccgct ggatcaacta tcagcagttt atccagacgc tggaaaaagc cattactcag
     5821 catcgccagc aacttaatca gtggacgcag aaagttgaca ttgccctgaa cagctggcga
     5881 gaaaagaaac aacgtttgca ggcctggcag acactgcagg aacggcaatc cacggcggca
     5941 ctgcttgcag aaaaccgcct cgatcagaaa aagatggatg agttcgccca gcgcgccgcc
     6001 atgaggaaac ctgaatgatt cgcttagcgc ccttgattac cgccgacgtt gacaccacca
     6061 cattgcctgg cggcaaagcc agcgatgctg cacaagattt tctcgcgttg ttgagcgaag
     6121 cattagcagg cgagacaact accgacaaag cggcccccca gttgctggtg gcaacagata
     6181 agcccacgac aaaaggcgag ccgctgatca gcgatattgt ttccgacgcg caacaagcta
     6241 atttactgat ccctgtggat gaaacaccgc ctgtcatcaa cgacgaacaa tccacatcaa
     6301 caccgttaac caccgctcag acgatggcgt tggctgcggt ggctgacaaa aatacgacaa
     6361 aagacgaaaa agcggatgat ctgaatgaag acgtcaccgc aagcctgagc gccctttttg
     6421 cgatgttgcc gggttttgac aatacgccca aagtgactga tgtgccgtca accgtgttac
     6481 cggcagagaa accaacgctc ttcacaaaac tgacttctgc gcaactcaca acagcacagc
     6541 ctgatgatgc ccccggcaca ccagctcagc cattaacacc gcaggtagca gaagcccaga
     6601 gtaaagcgga agtcatcagc acgccttcgc cggtgactgc tgccgccagc ccgctaatca
     6661 ctccacacca gacacagcca ctgcccaccg tcgccgcgcc tgttttgagt gcaccgctgg
     6721 gttctcacga atggcaacaa tcattaagcc agcatatttc gctgttcacc cgccaggggc
     6781 aacaaagtgc agagttgcgt ctgcacccgc aggatttagg tgaagtgcaa atctccctca
     6841 aagtggatga taaccaggcg caaatccaga tggtttcatc gcatcagcat gtacgcgccg
     6901 ccctggaagc agcgctgccg gtactgcgta cgcagctggc cgaaagtggc attcagttag
     6961 ggcaaagcaa catcagtggc gaaagcttta gtggtcagca gcaggccgct tcccaacaac
     7021 agcaaagcca acgcacagta aaccatgaac ctctggcggg ggaagacgac gatacgcttc
     7081 cggttcccgt ctctttacaa gggcgcgtaa caggcaacag cggcgttgat attttcgcct
     7141 aacgtcagag gtagcaccgt aatccgcgtc ttttccccgc tttgttgcgc tcaagacgca
     7201 ggataattag ccgataagca gtagcgacac aggaagaccg caacacatga ctgattacgc
     7261 gataagcaag aaaagcaagc gatcgctttg gatcccgatt ctggtattca ttaccctcgc
     7321 ggcctgtgcc agcgcaggtt acagctactg gcattcgcat caggttgctg ctgacgacaa
     7381 agcgcagcaa cgcgtcgtgc cctccccggt cttctatgcg ctggatacct ttacggtcaa
     7441 tctgggcgat gcggatcgcg tgctttatat cggcataacc ctgcgcctga aagatgaagc
     7501 tacccgctcg cggctgagtg agtatttgcc ggaagtccgt agccgtttgc tgttactgtt
     7561 ttcgcgtcag gatgctgccg tactggcgac agaagaaggc aagaaaaacc tgattgccga
     7621 gattaaaacc acactttcca ccccgcttgt tgccgggcaa ccgaaacagg atgtcaccga
     7681 cgtgctgtat accgctttta ttctgcgata acgacatggg cgatagtatt ctttctcaag
     7741 ctgaaattga tgcgctgttg aatggtgaca gcgaagtcaa agacgaaccg acagccagtg
     7801 ttagcggcga aagtgacatt cgtccgtacg atccgaatac ccaacgacgg gttgtgcgcg
     7861 aacgtttgca ggcgctggaa atcattaatg agcgctttgc ccgccatttt cgtatggggc
     7921 tgttcaacct gctgcgtcgt agcccggata taaccgtcgg ggccatccgc attcagccgt
     7981 accatgaatt tgcccgcaac ctgccggtgc cgaccaacct gaaccttatc catctaaaac
     8041 cgctgcgcgg cactgggctg gtggtgttct caccgagtct ggtgtttatc gccgtggata
     8101 acctgtttgg cggcgatgga cgcttcccga ccaaagtgga aggtcgcgag tttacccata
     8161 ccgaacagcg cgtcatcaac cgcatgttga aactggcgct tgaaggctat agcgacgcat
     8221 ggaaggcgat taatccgctg gaagttgagt acgtgcgttc ggaaatgcag gtgaaattta
     8281 ccaatatcac cacctcgccg aacgacattg tggttaacac gccgttccat gtggagattg
     8341 gcaacctgac cggcgaattt aatatctgcc tgccattcag catgatcgag ccgctacggg
     8401 aattgttggt taacccgccg ctggaaaact cgcgtaatga agatcagaac tggcgcgata
     8461 acctggtgcg ccaggtgcag cattcacagc tggagctggt cgccaacttt gccgatatct
     8521 cgctacgcct gtcgcagatt ttaaaactga aacccggtga cgtcctgccg atagaaaaac
     8581 ccgaccgcat catcgcccat gttgacggcg tcccggtgct gaccagtcag tacggcactc
     8641 tcaacggtca gtatgcgtta cggatagaac atttgattaa cccgatttta aattctctga
     8701 acgaggaaca gcccaaatga gtgacatgaa taatccggcc gatgacaaca acggcgcaat
     8761 ggacgatctg tgggctgaag cgttgagcga acaaaaatca accagcagca aaagcgctgc
     8821 cgacgcggtg ttccagcaat ttggcggtgg tgatgtcagc ggaacgttgc aggatatcga
     8881 cctgattatg gatattccgg tcaagctgac cgtcgagctg ggccgcacac gaatgaccat
     8941 caaagagctg ttgcgtctga cgcaagggtc cgtcgtggcg ctggacggtc tggcgggcga
     9001 accactggat attctgatca acggttattt aatcgcccag ggcgaagtgg tggtcgttgc
     9061 cgataaatat ggcgtgcgga tcaccgatat cattactccg tctgagcgaa tgcgccgcct
     9121 gagccgttag tgatgaataa ccacgctact gtgcaatctt ccgcgccggt ttctgctgcg
     9181 ccactgctgc aggtgagcgg cgcactcatc gccattattg ccctgatcct cgctgctgcc
     9241 tggctggtaa aacggttggg atttgccccg aaacgcactg gcgttaacgg tctgaaaatt
     9301 agcgccagtg cttcgctggg cgcgcgtgaa cgggttgtgg tggtcgatgt ggaagatgca
     9361 cggctggtgc tcggcgttac cgcagggcaa atcaatctgc tgcataaact tcccccttca
     9421 gcaccaacag aagagatacc gcagaccgat tttcagtcgg tcatgaaaaa tttgcttaag
     9481 cgtagcggga gatcctgatg cgtcgtttat tgtctgtcgc acctgtcctt ctctggctga
     9541 ttacgcccct cgccttcgcg caactgccgg gtatcaccag ccagccgctg cctggcggtg
     9601 gacaaagctg gtcgctcccg gtgcagacgc tggtgttcat cacctcgttg acgtttattc
     9661 cggcaatttt actgatgatg accagtttca cccgcatcat cattgttttt ggtttattgc
     9721 gtaacgcgct gggaacaccc tccgcgccac ctaaccaggt attgctgggg ctggcactgt
     9781 ttttgacctt ttttattatg tcaccggtga tcgacaaaat ttatgtcgat gcgtaccagc
     9841 cattcagcga agagaaaata tcaatgcagg aggcgctgga aaaaggggcg cagccgctgc
     9901 gtgagtttat gctgcgtcag acccgtgagg cagatttagg gttgtttgcc agactggcga
     9961 ataccggccc gttgcaggga cctgaagccg tgccgatgcg cattttgctc ccggcctacg
    10021 tgaccagcga gttgaaaacc gcatttcaga taggcttcac gattttcatc ccttttttga
    10081 ttatcgacct ggtgatagcc agcgtgttga tggcattggg gatgatgatg gttccccccg
    10141 ccaccattgc tctgcccttt aaactgatgc tgtttgtact ggtggatggc tggcaattgc
    10201 tggtcggttc gctggcgcag agcttttaca gctagagagg caaaatgaca cctgaatcgg
    10261 tcatgatgat ggggactgaa gcgatgaaag tcgcgctggc actggctgcc ccgctattgt
    10321 tggtagcgtt ggtcacgggc cttatcatca gtattttgca ggccgccacg cagattaacg
    10381 aaatgacgct gtcgtttatt ccgaaaatca tcgccgtatt tatcgccatt attattgccg
    10441 gaccgtggat gctcaatctg ttgctggatt acgtccgcac cttgttcact aacctgccgt
    10501 atatcatcgg gtagccgtac tatgttgcag gtgacaagcg aacaatggct atcctggtta
    10561 agcctgtact tctggccgtt actgcgcgtg ctggcgctga tctccaccgc gccgattctg
    10621 agcgaacgca gcgtaccgaa acgggtaaaa ctgggtctgg caatgatgat cacgttcgcc
    10681 attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg
    10741 gccgtgcagc agatcctgat cggcattgcg cttggtttta ccatgcaatt tgcctttgcc
    10801 gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt tgcgacgttt
    10861 gtcgatccgg ccagccatct taatatgccc gttttagcgc gtatcatgga tatgctggcg
    10921 ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct ggtcgatacc
    10981 tttcacaccc tgccgattgg tggcgaaccg ttgaacagca atgcgtttct ggcactcacc
    11041 aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cgttaccgct cattactctg
    11101 ctgctgacac tgaatctggc attaggttta cttaatcgta tggccccgca attatccatt
    11161 tttgttattg gatttccatt aactctgact gtcggcatct ctttaatggc ggcattaatg
    11221 ccgttaattg cgcctttttg cgaacattta ttcagtgaaa tttttaattt gctggctgat
    11281 attattagtg aattgccatt aatataattc cgtaacgttt atcatgttat cctaaggatt
    11341 atccgaaaaa taatacctac gaacatcttc caggatactc ctgcagcgaa atatttgttt
    11401 taagctcact cacatatcgc aacatttact ttactttaag acaattccag gcaaattata
    11461 caacacttta cgggatagta agtccgcctg aaaaatcgcg agagtggcgc attaggtgac
    11521 ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa ggggaattat cgttacgcat
    11581 tgagtgaggg tatgccatgt caacgattat tatggattta tgtagttaca cccgactagg
    11641 tttaaccggg tatctgttga gtagaggggt taaaaaaaga gaaatcaacg acattgaaac
    11701 cgttgatgac cttgccatag cttgtgattc acagcgccct tcagtggtgt ttattaatga
    11761 ggactgtttc atccacgatg cttctaacag tcagcgtatc aagctcatca ttaatcaaca
    11821 tcccaatacg ttatttatcg tttttatggc aattgccaat gttcattttg atgaatatct
    11881 attggtcaga aaaaatttat tgatcagttc taaatcgatt aaaccggaat ctctcgacga
    11941 tatccttggc gatattctga aaaaagagac aacgataacc tcgtttttaa atatgccgac
    12001 gttatcattg agccgaaccg aatcgagtat gttgcgaatg tggatggcag gtcagggaac
    12061 cattcaaatc tccgaccaaa tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa
    12121 tattaaacgt aagatcaaaa cgcataataa acaggttatc taccatgtcg tccgactgac
    12181 ggataatgtg actaatggta tttttgtcaa catgcgctaa cacattctga ctggtggttt
    12241 cccaccagtc aggctgaatt agattactct gctttctcca caaagatacc gtcctgatgc
    12301 cctgcttcat taaagaacca gatgccgagc gggtagtctt ccagcgaaac caggtacatt
    12361 gtgccttcac taaactcctc aactgccagt accacgccag gacgacgcgg accgccatcc
    12421 gttttgactg ttacccgatc attcaccttc atcgttttcc tcctgtggct ttgtgccagt
    12481 gtagaacaat ttcgtttttc tggcagcgcc aggcgcgcgc gagtgctgat tttctcgacg
    12541 gtctatactt aagagatgcc aggcggactt aacgactggc ggcaacaaca gagtaacggt
    12601 tgcgaggaaa gatgatgaaa accgcaaaag agtacagcga taccgcaaaa cgtgaggtca
    12661 gcgtcgatgt cgatgccctg ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc
    12721 gcagccagaa cgattctgaa cacgttagcg tcgatggacg tgaatatcat acatggcgtg
    12781 aattggcgga tgccttcgaa ctggatattc atgacttcag cgtctctgaa gtgaatcgtt
    12841 gaatgcacaa taaaaaaatc ccgaccctga gggggtcggg atgaaacttg cttaagcaag
    12901 aagcacttaa aaaattcgtt acaccaggaa atctgatgtg ttcatcacct tatccgcaat
    12961 ttttttcgct gacaagaaaa tattcgctat atgaatgatt aacgctactt atgaatagaa
    13021 atgtgacgca cgtcacccct tggtggctct ggcgtcagcg ggatcgctcc cgaaaaaatc
    13081 atctttgata atttgtgctg ttttgagaat catcctggta aggggagtac tgcgcgacta
    13141 tgttttcaat tcaacaacca ctactggttt ttagcgatct tgatggcacc ctgctggaca
    13201 gtcatagtta tgactggcaa ccggcagccc cctggctcag ccgtttacgc gaagcaaatg
    13261 ttcccgtcat tctctgtagc agtaaaacat cagcggaaat gctgtacttg caaaaaatgt
    13321 tggggctaca aggtttaccg ctgattgcag agaatggcgc agtgatccag cttgctgaac
    13381 aatggcagga gatagacggt tttccacgca tcatctcagg tattagccat ggcgaaatca
    13441 gccaggtttt aaatacgcta cgcgagaaag aacattttaa attcacgact tttgatgatg
    13501 tcgacgatgc aaccatcgcc gaatggacgg gattaagccg tagccaggcg gcgctgacgc
    13561 agcttcatga ggcgtcggta acgctaatct ggcgcgacag tgacgagcgt atggcacaat
    13621 ttaccgctcg tctgaacgaa ctgggcttac agtttatgca aggtgcgcgc ttctggcacg
    13681 tcctggatgc ctctgccgga aaagatcagg ctgccaactg gattatcgcg acctatcaac
    13741 aattgtcagg caaacgccca accacacttg gcctgggcga tgggccaaac gatgcgccct
    13801 tactggaggt aatggattac gcggtgattg tgaaagggct aaaccgtgaa ggggtgcatc
    13861 tgcatgatga ggatccggcc cgcgtctggc gaacgcagcg tgaaggaccg gaaggatggc
    13921 gtgaagggct ggaccatttt ttctccgccc gttaagcgtt atcgctcgcg aatacccgat
    13981 tgcgcccagc ctgtttagcg agataaagcc gacggtcggc cagtgactgg agttgttcaa
    14041 aatcataatc accggtttcc tcgctgctac ttacccccaa cgaggcactg atgcgtatcg
    14101 tcgtactctt agcaatcaac atctcttttt catttaactt caggcgaata cgttctgcga
    14161 cttccgcagc ctccgtcaga ctcgcgcctg gcagaatcac acaaaactcc tcaccaccga
    14221 cccgcccggc aacgtcctgc gcacgcaagg aactgctaat taatccggca gcatgagaaa
    14281 gaacacggtc gcccgcctga tgaccaaagc ggtcattaat cgctttaaaa tggtcaagat
    14341 cgacctgaat gacagaaaaa ggatgttggt gcgtctgaca caatttcgcg agcggacggg
    14401 ctttttcgaa cagtgcgcca cggttatata aacgcgttaa ggtgtcgtgc cacgcctgcc
    14461 actgcaacga gctttgcaga acatacatgt tgctgaccat ccggcgaatc acataccagg
    14521 agatgagtaa catggtggta aagagcgccc acagcagggt taatgcaatg ctgatactgc
    14581 cgaaatcgcc gcgcacgcct tcgcttaacg tatggacgcg caccagcaca ccgtcgaaat
    14641 ggtccagacg ttcccagcta acatagcgac tgtccatacg aatgccgccc cgcgtgtcat
    14701 gttccattgc ctgcgccagc aaggccagtt cacgaggatc aaaaatattc cctgttggat
    14761 ggtcaggatt ggaagaggtc aaaaatctca gcttactgtc atagagctga tactcaccat
    14821 cgaggttttt atcgatggcg tttcttaaaa attgctgcat agtacgcacg ggaatactca
    14881 tccccagcac gccataccag tagttattac tgtctaccgg aacactgacg gtaacctgcg
    14941 gttcagtatt gctggcgtgt tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat
    15001 tttctcgttg tgaatggccg ataaaccagg gttgggtgac atagccgtaa taacgcgtcg
    15061 ggacattgcg cgtaaacaag gtcggctgcg tcgaaacgta aaatccggca cgcgagacat
    15121 acatcgcctg ttctaccatc gacgaggagt tgtgtgccag tcgcagcaag taaccaactt
    15181 ccagtgcagc ggtaatttca ttgtcgaggc tttcattttc gcgagacagg agattcccct
    15241 cgctgactaa tgcatccgat acgccattga ctgacagggt gcgtcgtcgg ttgagttcga
    15301 tttgccaggc gtgctcgtcg cgatgctgtt cgaactcggt tacagcatca cgcagagagg
    15361 tgaaatccag tggcgctacg agagcttcgc gcatgccatt acgcaaaaag atcagtttgt
    15421 cgacgttata ctgcaaatgc ttatcgagcg cgttggctac gttttccaga tgattacgct
    15481 ggctggagat ataggcatct tccagcacca ctacttcacg ccaggtcagc aaggtggaaa
    15541 aaagcaatac cacgataaag cagagattaa cgatatgacc aggccctagg cggcgtgcga
    15601 gttttttcaa ccagctctgg ttttccattt ttgtctcgtg ctgcaccctg acgactcccc
    15661 acctgctttt ttatgattct ggcataacct ggcgatagcg taccgcaaaa cagcgataat
    15721 tttataatgg tggcatgata agacgcgact atcattcatt gcaggcattg gcacaccagg
    15781 ccggatgtga tccggcctgg tcttaatgat accctttcaa acttcttccg cagaacgact
    15841 cccctcccct ggaacaagct cggtttcatg aaaagcttcc cgcttgaccc cgtaaccgtc
    15901 ataccaacga cactcaacca taccgctgga gtatccagtg acaatcatcc gcgggccgcc
    15961 ctctttaacc gtaacttcct cactaaccat aaagctcata ctcgcctcct tttttcgagt
    16021 gaaacttgtt caccttagtt gaagatggcg aattttgcat aatagcctgt gcgagtatta
    16081 atgtacctga ccgcgcattt tcgctacggc tttcactccc agcaccacga tgccgccgat
    16141 gataaatcca agaatcagat ttaaaacagt cggtaatatc atcgccacca ctgcactttg
    16201 ctgcccggcg aaatgttcaa tggcgtgatg cagcggcgca ataccatgta ccacaatccc
    16261 gccgccgacg aggaacatcg ccagcgtgcc gacaatcgat aacgctttca tcagccaggg
    16321 cgcgataatc aataatcctt tacctaatgc ctgcatcagc gcgctggatt tttccgccag
    16381 ccaatacccc aggtcatcaa tcttaacgat aaccccgacc agaccgtaga cgcccacggt
    16441 cactaccagc gcgatgcctg aaagcaccag cacctgatta agcaacggcg cttcggccac
    16501 aatccccagc gtgatggcga cgatttccgc agacaatata aaatcggtac gaatcgcccc
    16561 ttttattttg tccttttcaa acttcagcgg atcctgcgcc gccagcttct ccagacgctg
    16621 ctggctctgc gccggatctt ctttatgttt acgcgcctcc agcatatgca gcactttctc
    16681 taccccttca aagcagagaa acgcgccacc gatcatcaac aatggcgtaa tcgcccacgg
    16741 gataaacgca ctgatgatca gcgccagtgg caccagaatc actttattaa tcagcgatcc
    16801 tttcgccacg ccccagacca cgggaagttc ccggttggcc cgcacgcctg aaacttgttg
    16861 cgcattgagc gataagtcat cccctaatac accggcggtt ttcttcgccg ccagtttgcc
    16921 catcacggag atatcgtcca gcagtgtggc gatatcatcg agcaacgtca gtaaactact
    16981 cccggccaaa agcattccct ctcattttta ttgttgaagg gtgtaagtat gaagcaaaaa
    17041 cggcagctct gatacttgct tcacaggtca acattttttt aacaatcaag tgcaattata
    17101 tctctcgcca gcagaatgat tttcacgttt actatgaacc acctttttat ttccaccgtg
    17161 agggattatg cgtttccgcc agttgttacc gctttttggc gcgctgtttg cgttgtatat
    17221 catttggggc tcaacctatt ttgtcattcg gattggcgtg gaaagctggc ctccgttaat
    17281 gatggcgggt gttcgattcc tggcagccgg tattttattg ctggcatttt tactactgcg
    17341 cggacacaaa ctccccccgc tacgtccgct gctcaatgcc gcgctgattg gcctgttatt
    17401 gctggctgtc ggtaatggca tggtgacggt tgccgaacat caaaatgttc cttccggcat
    17461 cgccgccgta gtggttgcaa ccgtgcccct ctttaccctg tgcttcagcc gcctgtttgg
    17521 cattaaaacg cgcaaactgg aatgggtggg tattgccatt gggcttgccg gaatcatcat
    17581 gctcaatagc ggtggaaatt taagcggcaa tccgtggggc gcgattctga ttttaatcgg
    17641 ctcgattagc tgggcgtttg gctcagttta tggctcgcgc attaccttac ctgtagggat
    17701 gatggcgggt gcgattgaga tgctggcggc aggcgtggtg ttaatgatcg cgtcgatgat
    17761 tgcgggtgaa aaactgacgg cgctcccttc cctttcaggc ttccttgcgg tcggctatct
    17821 ggcgctgttt ggttcgatta tcgccatcaa cgcttatatg tatttaatcc gtaatgtcag
    17881 tccggctctc gccaccagct acgcttacgt taacccggtg gtcgcggtct tgctgggtac
    17941 gggactgggt ggagaaacac tgtcgaagat tgaatggctg gcgctcggcg taattgtctt
    18001 cgcggtggta ctggtcacgt tgggaaaata tctctttccg gcaaaacccg tagttgctcc
    18061 agttattcag gacgcatcaa gcgagtaaat gtatcccctg cgtgtcgatc tgcgcgctgg
    18121 ctccttcgcc gcagatccac tcttccagac gctcggtaag cgcttcatcc ttcagcttct
    18181 cgcgcccacg taacgcgcac tcccagacaa tcaatacgcg ccagccgagt tcctgcaagc
    18241 gactgatatc gcggcgatcg cgctcaacat ttttacctat cttctccagc cagaactcgg
    18301 ttcgagtcgc aggcacttta aacagatagc agtgatgatg atgccagaag cagccatggg
    18361 taaatatcac gcagcgatat tcatcaacga caaaatccgg gcgtccgggc agactggcgt
    18421 cctgaacgcg aaacgccagg ccctgcccgg ttaacagact ggcgaggcgc ttctcaatcg
    18481 ccgtatcacg cgtggcaatc gcgcgcatat ttttgctgcg agtggcctta tcgtgaacgt
    18541 cggccatgtt gtgcctcttg ctgacgcaac gccaccgcct gtttgatttt tggctcaagc
    18601 agttttgcca ccgcggcaaa gaccggcacg accaccgagt taccgaactg gcgataggcc
    18661 tgagtgtccg aaaccggaat gcggaatttc gcttctcccg gcgcttcaaa acccattaag
    18721 cgcgcgcatt cccgaggcgt taaccgacgt ggacgatgtt gctgattcag cggatcgtca
    18781 aagtctttct cacccgtggc catatcccag ccgcgatcga ttaaaatttc cgcgccatct
    18841 ttgtaataac gcgcagacag cgtacgggtg acgctttgcg gattgttcgg ataaaccatt
    18901 ccataaccga agccgtttcc gcgcgcctga tgttttttcg catatcgata gaggtacttc
    18961 cacagcaccg gcgtcaggat atatttcgcc tcgaccatcg ggtccaacag ctgcgccagc
    19021 gtcactcgct gtgcagggaa acattcgctg atatcacgca gagtaaaatc ggctttcaga
    19081 ttaagatcgc gacgaaaacc caccagcacg atgcgttcac ggtgctgcgg cagaaaatgt
    19141 ttgccatcga tgattttcgg atcgtccggc ccgttatctt ctgcatcagc cacgtcatag
    19201 cccagttcgt ccagcgtctg catgatgatg cggaacgttt taccctggtc gtggcttttc
    19261 aggtttttga cgttttcgag cacaaacatc gccggacgac gcgcgtcgat aatgcgcacc
    19321 acatcaaaga acagcgtgcc ctgagtatcg caggcaaaac cgtgcgcccg cccgagcgag
    19381 ttctttttcg atacgccagc cagcgaaaat ggctgacaag ggaaaccggc cagtaaaaca
    19441 tcgtgttcag gaatgtgttg acgaatatgt tccgccgcag cctcatcact cacgccttct
    19501 ttatggctga gggtgatgtc gcggatatct tcattaaaat gatgcgtcgc cgggtcgcaa
    19561 taatgattgg ctttataagt gcgtaccgca tgtttgttcc attcgctggt aaacacgcac
    19621 tgtccgccaa tcgattcaaa accgcgacgg atgccgccaa ttccggcaaa tagatcgata
    19681 aagcgaaacg cataatgcgg atgatgtgcc ggtggtttgg gtaacaacgt ttgcagatgg
    19741 gcgaactctt tctcacttaa acggtgccat gccgagtcat tcgccagcgc acgttttaaa
    19801 attgccgcgc tccagtgatt ctcacccaca ccattaagct gagccaccag cgttttaaca
    19861 tcataaattt gcagcagttt ctccagcatt gcctgtgcgg cattcccggt gctgtatgaa
    19921 tcggttactg atatattttc ctgcatagat ttaaccggcc atctgagatg gtcgacaggt
    19981 taacataatt acagccattt agggccacgc gctcagcccg cgggtgaaaa cgcatctatc
    20041 accttgtgat cgacaccttc attctccccc gccagttcgg cactgagctt cgccataaac
    20101 tcgactaaaa aatgcgcgtt gtgctgcgcc agctgcttgc ctcttacggt ttgcatgatt
    20161 tgcggtagct tcaacaactt agtctggaaa tgatccagcg cgtagcgttt atcatcaagc
    20221 gggcgatgct gtgcgagcgg atcttcggca tcaaacagcg ccacgcccaa tgcccctgaa
    20281 acggcaaaca cgcgcgccag gccaatcgcc cccagggctt ccagccgatc cgcatcctgg
    20341 acgattttag cttccgttgt taagggggcg atttgcgcgc tgaaactgtg agcggcaatg
    20401 gcatgacaaa cggcctcgat tttctccgcc ggaaattgca cgaactcttc acggagcagg
    20461 cgacgcgtct cttctgccgc caggattgaa gaactctgcc gttgcggatg atttttcgcc
    20521 aggctcacaa tatcgtggaa ataacaggcg gttaaaatca ccagcatatc gacgtcatca
    20581 tctgccgcca gcttctgtgc cgtagcccag acgcggcgaa aatgacacac gtcatgggcc
    20641 gcgtcctggt gctgatgatg gtttttcaac cagttttcga actgtgcctg ccagtgttgt
    20701 aagtccatac gcgctccgtt gccaaagagt cgccacctta gcagttttta ttcatagcgt
    20761 ctgctacgtg cgggacgagg tcgtgtgtac caggcaatgc tgcctaacaa tgccccgacc
    20821 agagccataa ataaaaatcc gaccagtgcc ctaaccactt tcccgcggta gagcctaaat
    20881 cgctttcaaa tcccgacacg ggggcatcgg ggatccggct catcacccag atgtaacgcg
    20941 ccattgccca gacgaaatag ccacaccatg tataaaaagc ccacaaagcc agtttgccgc
    21001 cagggctgcg agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac
    21061 ccggaaaatt cagacttagt actattacgt gataaaagtc acatttttcc acattggatg
    21121 agccagtcaa gactcactca ttttttgaac a
//
LOCUS       DAZKIF010000084        20887 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZKIF010000084 DAZKIF010000000
VERSION     DAZKIF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.084"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..329
                     /locus_tag="ACHOTW_003072"
     CDS             81..329
                     /locus_tag="ACHOTW_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9845132.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHOTW_003073"
     CDS             307..1437
                     /locus_tag="ACHOTW_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9845133.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHOTW_003074"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHOTW_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP9845134.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHOTW_003075"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHOTW_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHOTW_003076"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHOTW_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP9845135.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHOTW_003077"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHOTW_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP9845136.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHOTW_003078"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHOTW_003078"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP9845137.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHOTW_003079"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHOTW_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP9845138.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHOTW_003080"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHOTW_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP9845139.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5873..7126
                     /gene="kch"
                     /locus_tag="ACHOTW_003081"
     CDS             5873..7126
                     /gene="kch"
                     /locus_tag="ACHOTW_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP9845140.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7181..7354)
                     /gene="yciY"
                     /locus_tag="ACHOTW_003082"
     CDS             complement(7181..7354)
                     /gene="yciY"
                     /locus_tag="ACHOTW_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP9845141.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7497..8957
                     /gene="cls"
                     /locus_tag="ACHOTW_003083"
     CDS             7497..8957
                     /gene="cls"
                     /locus_tag="ACHOTW_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9845142.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8992..9321
                     /gene="yciU"
                     /locus_tag="ACHOTW_003084"
     CDS             8992..9321
                     /gene="yciU"
                     /locus_tag="ACHOTW_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP9845143.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9374..10378)
                     /gene="oppF"
                     /locus_tag="ACHOTW_003085"
     CDS             complement(9374..10378)
                     /gene="oppF"
                     /locus_tag="ACHOTW_003085"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP9845144.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10375..11388)
                     /gene="oppD"
                     /locus_tag="ACHOTW_003086"
     CDS             complement(10375..11388)
                     /gene="oppD"
                     /locus_tag="ACHOTW_003086"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP9845145.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11400..12308)
                     /gene="oppC"
                     /locus_tag="ACHOTW_003087"
     CDS             complement(11400..12308)
                     /gene="oppC"
                     /locus_tag="ACHOTW_003087"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP9845146.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12323..13243)
                     /gene="oppB"
                     /locus_tag="ACHOTW_003088"
     CDS             complement(12323..13243)
                     /gene="oppB"
                     /locus_tag="ACHOTW_003088"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP9845147.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13329..14960)
                     /gene="oppA"
                     /locus_tag="ACHOTW_003089"
     CDS             complement(13329..14960)
                     /gene="oppA"
                     /locus_tag="ACHOTW_003089"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP9845148.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(15698..16345)
                     /gene="ychE"
                     /locus_tag="ACHOTW_003090"
     CDS             complement(15698..16345)
                     /gene="ychE"
                     /locus_tag="ACHOTW_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP9845149.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(16376..16429)
                     /gene="ychE"
                     /locus_tag="ACHOTW_003091"
     CDS             complement(16376..16429)
                     /gene="ychE"
                     /locus_tag="ACHOTW_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP9845150.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            16822..19497
                     /gene="adhE"
                     /locus_tag="ACHOTW_003092"
                     /gene_synonym="adhC"
     CDS             16822..19497
                     /gene="adhE"
                     /locus_tag="ACHOTW_003092"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9845151.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(19798..20415)
                     /gene="tdk"
                     /locus_tag="ACHOTW_003093"
     CDS             complement(19798..20415)
                     /gene="tdk"
                     /locus_tag="ACHOTW_003093"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9845152.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
BASE COUNT         5456 a         5088 c         4874 g         5469 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata cctgccccaa cataaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatgatg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaggc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagagaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaacg tgatttaaat
     2281 caatttttaa atattttcgc tacgcaaata ttaactcgtt cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgccaaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccatattact ggccctggca ccggtgatgc tggtacagga caaaatgggc gtttttgcct
     3001 cgatccgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttca gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caaaatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctgaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtcctttgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg caaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagac gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ctgtacttga tgtcgggcgt gccggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt ttttgctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc
     4801 ttcttttggc ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 cggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttacttta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gagtagagat
     5581 ccaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttataggtc
     5641 ggtcattgca ttcaccacca caagcagata ataactttat ctgtatttct ttatatgatg
     5701 tgagcatgtt caggaaaaat gccctttcct gacgcagagt tgctcactca aaacctttga
     5761 ttactgcgga taaagtcatc tactattgtt taacacattc acgtgtcgca tgtatgttca
     5821 gaaaatgtgc acgttaagat aattaaacaa aattgtggtt acagaggtat cagtgagtca
     5881 ctgggctaca ttcaaacaaa cagcaacaaa tttatgggtg accctacggc acgacattct
     5941 cgcgctggcc gtctttttaa atgggttgct tatttttaaa acaatctatg gtatgtcggt
     6001 caatttgctt gatattttcc atatcaaagc attttcagag ttggatctct ccttgctggc
     6061 aaacgcccca ctatttatgc tcggcgtctt tcttgtcctg aactccattg gcttactgtt
     6121 ccgggcaaag ctcgcatggg caatcagtat cattttgttg ttgatagcgc taatttacac
     6181 cctgcatttt tatccctggc tgaaatttag tattggattt tgcattttta cgctggtgtt
     6241 tttactgata ctgcgcaaag acttctccca cagtagcgcc gcagccggga caatttttgc
     6301 atttattagt ttcacgacat tactgtttta ctcaacctac ggtgcgcttt atttaagcga
     6361 aggttttaat ccgcgaatag aaagtttgat gaccgcgttc tatttttcga tagaaaccat
     6421 gtcaaccgtc ggctatggcg atattgtccc tgtttctgaa tcagcacgat tgttcactat
     6481 ttcggtcatt atttccggca ttaccgtttt tgccacatct atgacctcaa tttttggtcc
     6541 gcttatccgc gggggattca acaaacttgt aaaaggaaac aatcatacaa tgcatcgtaa
     6601 agatcatttt attgtttgcg gacattcgat tctcgccatt aatacgatcc tgcaactgaa
     6661 tcaacgcgga caaaacgtaa cggttatcag caacttgcct gaagatgata tcaagcaact
     6721 tgagcaacgc ttaggcgata acgctgatgt tatccccggt gacagtaatg acagttctgt
     6781 attaaagaaa gcgggaatcg atcgttgccg ggccattctg gcgctgagtg ataacgatgc
     6841 agataacgcg tttgttgtac tctcggcaaa agatatgagc agtgatgtca aaacagttct
     6901 cgccgtcagt gatagcaaaa acctgaataa gattaagatg gtacatccgg atatcattct
     6961 ctcaccgcaa ctgtttggca gcgaaattct ggcgcgagtt ttaaatggtg aagagattaa
     7021 taatgatatg ctcgtttcaa tgttgttgaa ctcaggtcat ggtattttca gcgataacga
     7081 tgaacaagaa acgaaagctg acagtaaaga atcggcgcaa aaatagagtt taaagacgga
     7141 agagtaaact tattgggcac cgcaatggtg cccagtacat ttagatattg tagatcgcaa
     7201 acaataacga gttacgctgt ttgtttagaa tgcacttcct gatgctgtgg atacgcatat
     7261 ttcggcgcga ctgcccctca agccaacgcg atttacgtcg gctagcctga cgctgcatgc
     7321 gccagcgccc cacttccgtt ctactccgct tcatgttaac tactctatgc aataacagaa
     7381 tggtcattat acccccgccc ccgacgctga ccagtgcttt tcccgcgttt ttatttgcca
     7441 gatgaatcca tatgcgtaaa ctcataacaa tgcgctttca aaaggatttc taatctatga
     7501 caaccgttta tacgttggtg agttggttgg ccattctggg atactggttg ctcattgcag
     7561 gcgtaacttt acgcattcta atgaaacgac gcgcagttcc ctccgcgatg gcctggctgt
     7621 tgattattta cattctgccg ttagtcggaa ttattgccta tcttgccgtt ggcgagctcc
     7681 atttaggcaa acgccgcgct gagcgcgcca gagcgatgtg gccttccacc gcaaaatggc
     7741 ttaacgacct taaagcctgt aagcatatct ttgccgaaga aaatagcagt gtcgctgcgc
     7801 cattattcaa gctttgcgag cgtcgtcagg ggatcgctgg ggtcaaaggg aatcagctac
     7861 aactgatgac cgagtcagat gatgtgatgc aggcgttaat ccgcgacatc cagctcgcgc
     7921 gccataatat tgagatggtg ttttatatct ggcagcccgg cggcatggcg gaccaggtgg
     7981 ctgaatcatt aatggcggct gcgcgacgcg gcattcattg ccgattgatg ctcgactccg
     8041 ccgggagtgt agcttttttc cacagcccgt ggcccgagct aatgcgtaat gccggtattg
     8101 aagtggtcga agccttaaag gtcaatctga tgcgtgtgtt tttacgccgt atggacctgc
     8161 gccaacatcg caagatgatc atgatcgata attacatcgc gtacaccggc agcatgaata
     8221 tggtcgaccc tcgctacttc aaacaagatg cgggcgtagg gcaatggatt gatctgatgg
     8281 cgcgtatgga aggccccatc gccaccgcga tggggattat ttattcctgc gactgggaga
     8341 ttgaaacagg gaaacgtatt ctaccgccac cacctgatgt caatattatg ccgtttgagc
     8401 aggccagcgg tcataccatt cacacaattg cttctggccc cggctttccg gaagatctca
     8461 ttcaccaggc attattgact gcggcttatt cggcgcgtga atatttgatc atgaccacgc
     8521 cctactttgt gccaagcgat gatttacttc atgcgatttg cacggcggcg cagcgcgggg
     8581 tggatgtcag tattatcctt ccgcgaaaaa atgactcgat gctggtcggc tgggccagtc
     8641 gcgcattctt tacggaactg cttgctgctg gggttaaagt ttatcagttt gaaggcgggt
     8701 tactgcatac caagagcgtg ctggtcgatg gcgaactaag tctggttggc accgttaacc
     8761 ttgatatgcg tagtctgtgg ctgaatttcg agattaccct ggcaatcgac gataaaggtt
     8821 ttggtgcaga ccttgccgcc gttcaggacg attatatttc gcgttcacgt ctgctcgatg
     8881 cccgtttatg gctaaaacgt ccattatggc aacgtgtcgc cgagcgactg ttttacttct
     8941 tcagcccgtt gctgtaaaac gtcgccaaca gaggttaaac aggtagtgac tatggatatg
     9001 gatctaaaca atcgcctgac tgaagatgaa acgcttgagc aggcctacga tatttttctc
     9061 gaactggctg ccgacaatct cgatccagct gatgttctgc tgttcaatct tcagtttgaa
     9121 gagcgcggcg gcgcggagtt attcgatccg gcggaagact ggcaggaaca tgttgatttc
     9181 gatttgaacc cagacttttt tgccgaagtt gtgattggtc tggcggacag tgaagatggt
     9241 gaaatcaacg atgttttcgc ccgcattctg ttatgccgcg aaaaagatca caaactttgc
     9301 catatcatct ggcgggaata aataatcact ttcggcgtga aaaagggctg acagctgtca
     9361 gccctttttg tgtttaaagc ggatcgactt tcaggcaaga aacggcgtgt ctgaaactcc
     9421 cctccagcac aggacgtgtt ttggcacact ccggaccggc aatcgggcaa cgggtacgga
     9481 aaacacaacc ggaaggcggg ttgatcggcg acggtaattc cccttccagt aactggatgg
     9541 ttttgttctt ctccagatcc ggatcaggta tggggactgc cgacatcaat gccctggtgt
     9601 aaggatgtag tggattgtgg tagacctcat cataggtccc cagttctacc gcatggccga
     9661 gatacatcac caacacacga tcggaaatgt gttttaccac ggccaggtca tgagcgataa
     9721 aaattaatga caatcccatc tcacgttgca attgctggag caggttgacc acctgtgcct
     9781 gaattgacac gtccagcgcc gacaccggct catcacagat aatcagcttc ggttcaagaa
     9841 taagagcacg cgcaatcccg atacgctggc actgcccacc agagaactca tgcggatagc
     9901 ggttaatcag gttaggcaat aacccgactt tcagcatcat cgccttcacg cgctcgcgaa
     9961 cttcctggcg tgacattttc ggatgataag tacgcagtgg ttcagcgatg atctcgccga
    10021 tggtcatacg cgggtttagc gatgccaacg gatcctggaa aatcatctga atatcactgc
    10081 gaacggcacg ccattcatcg ggtttcatgc ccagcaactc tttacctaac caggcaacat
    10141 gaccgtcggt cgctttaacc aggccaatga tggcgcgagc aaaggtggac ttaccgcatc
    10201 ccgattcccc taccacacct aatgtttccc cttcatacag gcgaagagtt acaccatcta
    10261 cggctttgag cgttttcggc ggttgccaga accactgttt gccatctttg atttcaaagt
    10321 gcactttcag atcggcgatt tcgaggagga cttttcttcc ttcggttaca gcattcataa
    10381 cagttcctcc accggtttaa agcaagcacg cagacggcca ggcgtaaact cttccagcgg
    10441 cggtgcgcta ctacaaattt ccatcgcatg cggacaacgt ggctggaatg ggcaaccttt
    10501 cggtaatcgc agcaggtttg gcggattacc cggaatagtc aacattgttt caccttccgc
    10561 atcgagacga ggcaccgcgt tgagcaaacc gatagaataa ggatgaacgg gttgataaaa
    10621 gacatcgcgc gcgttgccat attccatcgt gcgcccggcg tacattacca gcactttgtc
    10681 gcagatcccc gccaccacgc caagatcgtg ggtgatcata atgatggcgg tattaaattc
    10741 ccgcttcagt tcattcaata gcgtcatgat ctgcgcctgt acggtgacgt ccagcgcagt
    10801 agtgggttca tccgcaatca gcagcttagg tcgacatagc aatgccatcg caatcataac
    10861 tcgctgacgc atgccgccag aaaattcgtg cgggtacatt ttcatgcgtt tacgtgcttc
    10921 cggcattttt accgcatcga gcatccgcac cgactcttca aacgcctcag ctttgctcat
    10981 gttcttatgc agcatcagca cttccatcaa ctgctcaccg acgcgcatat agggattcaa
    11041 cgaagtcatt gggtcctgaa aaatcattga gatttgttca gcgcgcagtt tattgagttc
    11101 acgctctggc aaattgagga tttcacgccc attgaaggtt gccgatccgc caatgcgtcc
    11161 gttggcagcc aacaggccca tcaacgcaaa tgcagtttgc gatttacccg aaccagactc
    11221 acctacaatg cccagcgttt ctccggcacg tagggaaaaa tttaaatcat tgaccgccgt
    11281 gacgtcgccg tccggggtac taaaggtgac acgcaaatct ttcacgttca gcagtgcgtc
    11341 agcctgttgt tgtgcgagcg gcacagttgc agtttcaatt acgctcatgg ctgcactcct
    11401 taacgatctt tcgggtcgag ggcatcacgc aagccatcgc cgataaagtt gaaacaaaac
    11461 agcgtcacca cgaggaatcc cgctgggaac aacagtaacc atggagagac ttccattgag
    11521 ttcgcgccat cactcagcaa tgccccccag ctgcttaacg gctcttgcgt acccaacccc
    11581 aggaagctaa ggaaagattc aaagaggatc atgctgggca ccagtagcga tgcgtagacc
    11641 accaccacac cgagtacgtt cggcacaatg tggcgaataa caatgcccga cgtcgataca
    11701 ccgccaactt gtgccgcctc aataaactct ttgcgcttca gactcagggt ttgcccacgc
    11761 acaatacgag ccatatccag ccaggaaacc atgcctatcg ccacgaaaat cagcaggatg
    11821 ttttgaccga aaaaggtcac cagcaaaatg acgaagaaca tgaatgggaa ggagttgagg
    11881 atttccagca aacgcatcat taccgaatcc actttaccgc ccagataacc ggaaagcgaa
    11941 ccataaagtg tccccacgac cactgccacc agtgccgcag caacaccgac catgagtgag
    12001 atacgtccgc caatcgcaac gcgcacaagc aagtcgcgac cggatgagtc agtaccaaag
    12061 tagtgaccgg actccatatc cggggcgctg gacatcatcg cccagtcagt atcgtcatag
    12121 gcaaactgcg aaagcatcgg tgccaggatt acaaataacg cgatcagcac cagcactatc
    12181 agacttgcaa ccgccgcacg gttatgcata aaacgtcgac gtgcgtcctg ccacaagctg
    12241 cgcccttcga cctccagctt ttcactgaaa ttttccagcg tctcgctgtt tttcttactt
    12301 aacatcatcg cgagctccag tatcagtaac ggattttcgg gtcgataacc gcatatagca
    12361 catcgacaat ggcattaaac aaaatggtta acgcaccaac caggatggtc aggcttaaca
    12421 ctaaggaata gtcacggttc aatgcgccat taacgaacag ttgcccaatc cccggcaaac
    12481 cataaatggt ttcgataacc atagaaccgg taataatgcc gacaaatgcg gggcccatat
    12541 aggagagcac gggtaacaga gcaggtttta atgcgtggcg taaaatgatc cgccgcatag
    12601 gtaacccttt cgcccgggca gtacgaataa agttggagtg taatacttca atcatagagc
    12661 cacgggtaat acgcgcaata ctggcgatat aggcgagtga caacgccacc atcggcaata
    12721 tcatgaattt aagcgcccca ccattccagc caccgcccgg cagccaatgc aaaatgatcg
    12781 caaatatcat gactaataat ggcgcaacca caaaactggg gataacaacc ccggtcattg
    12841 ccagccccat cacggtatag tcccatttgg tgttttgttt taatgcggca ataacgccag
    12901 cactaacacc cagtattacc gcaaggaaaa atgctgcggc tcccagtttg gcagaaacgg
    12961 gaaaactgga tgcaaccagg tcattgaccg aataatcttt atatttaaac gatggaccga
    13021 aatcgccatg cgccagttgt ttcaggtagc tgaaatactg tgtcatgatc ggatcattaa
    13081 gatgatattt cgcttcgata ttggccatta cttccggcgg taaagtacgt tcgccggtaa
    13141 aagggcttcc cggcgcgagg cgcatcataa agaacgaaat agtaataaga ataaatagcg
    13201 tcggaatcgc ttccagacag cgacgtagaa taaattttaa cattgcccta ccttctggcc
    13261 tgtgccttct gtaatgcgat aaaaatcaga caccgtggag caggacactc ctgccccacg
    13321 tattgccatc agtgcttcac aatgtacata ttccgggtgt aggtattatc cagcggatct
    13381 ttgccggtat aaccaccaac ccacggtttc accagacgcg cattcacgta gtaataaaca
    13441 ggaacaatgg ccgaatcctt atccagctgt tgttctgctt tagtgtacag agctgtgcgc
    13501 tgcgcctcgt cagtcacttt cagcgtttcc gccataatgc tgtcaaaggc cgggctctta
    13561 taatgcgcgg tattcatcga gctgttcgac agcatggtgt tcaggaagga agttggttcg
    13621 ttgtagtcag cacaccagcc tgcacgggcc acatcaaaag taccctggtg acgggtgtcg
    13681 aggaacgttt tccactcctg gttaaccagt ttgacgttta caccaatgtt tttcttccac
    13741 aatgaagagg cagcaatcgc cagcttttta tgcagatcgg aggtgttata caacaggttg
    13801 atggtcaacg gtttgtctgc ggtataaccc gcttcagcca gcagtttttt cgcttcttcg
    13861 ttacgttttt cctggctcca gccaaaccat tccggctgag tcaattttgc gccatcagta
    13921 tacggtggag tgtaaccata ggcgggcatg tcgccctgcg ctttcacttt attaacaatg
    13981 atatcgcggt ccatacccag tttcagtgcg gtacgcacac gcacatcgtt gaatggcggt
    14041 ttctggttgt taatttcgta ataataagtg cacaggtatg gatcaacgtg aacttcgtcc
    14101 gggatctctt ttttcagctt ctggaacaat tcgatcggca tgttgttata agtcatgtcg
    14161 atttcaccac tacggtagcg gttgacatcg gtaacttcag aagcaatggg caaataggtt
    14221 acctggttaa taacggtttt cgcgttgttc cagtaggttg ggctgcgttc aagaacgatt
    14281 cgttcgttta cgacccaatc ttttaaggta taggcaccgt tggtgacgat attaccaggc
    14341 tgggtccatt tttcgccgaa tttctcgata gcggcttttg gcaccggtga agttgatggg
    14401 tgaacaagta atttatagaa gtacggaacg ggttcactta aggtgacttc taatgtgtga
    14461 tcatcaatag ctttcacgcc gagatcggta atcggttttt tcccttcaag aatttcatca
    14521 ataccggcga tatgcccata ttgcagataa ctggcatacg gagaagcggt gttcggatca
    14581 acagaacgtt gccagctata cacaaagtct tgtgctgtga ctggcgtgcc atcagaccat
    14641 ttcgcatctt tacgcaaatg gaaggtccag actttcgcgt ctttattatc ccaggattca
    14701 gcgacgccag gtgccggatg gccgtcaaga tcgctgacca gtaagccttc aaacaggtct
    14761 cggctgatat tagactccgg aacaccttca attttgtgcg gatctaatga ctgaacttct
    14821 gaaccattgt tacgtaccag cgtttgtttt tccgccagtg tgacgcctgc gggtacatca
    14881 gctgccagcg cgacattccc tgccattagc gcagccagaa cgccagctgc tactaaactt
    14941 ctcttggtga tgttggtcat tgttttttgg actccctcat tataattact ggtattaacc
    15001 agcatgtgta atcccggatt gggaccctgc acaggctcgg agactttctg ctgtcaggtt
    15061 actttatcgt tacggattgc tatcaccgac tttatttatt ctggtgattc agacgatcac
    15121 cttatgtcga ttcgtactgt gtctcccctg tcgagacaca ttattctttc gcgacgctct
    15181 ttaattgtta attattctca ttaccaataa agaatcgcct gacggttgcc cggaaagtac
    15241 caaatgcggt tcttactcgc caatacattt tgcaaaattg ttaagcaatt ctcttttatt
    15301 gaccgtatca gaatctatag cttgcatcca tgtcttaaca aacatcttta aattcaatca
    15361 aatagagatt ggttaagcat gtcgatcttt tctgattaca gttccagtag tgaaatgcac
    15421 aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag tggaattact
    15481 aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa aataacatga
    15541 tgatgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag aagaatattt
    15601 ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata atgacgaagt
    15661 aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg ccggggaaaa
    15721 tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat agccccataa
    15781 tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac ggtgccatgc
    15841 ggaacaatcc ccaacaacat aaagcgaaca atgcaatagc cacaaagaaa ccaaacagat
    15901 agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc cctggccccg
    15961 ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg gtttctgatt
    16021 tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac atcgctatcg
    16081 tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca aaaagttgca
    16141 gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac aggttggctg
    16201 taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg ataaagacgg
    16261 gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg aagtaaacgg
    16321 gaaaatcaaa aagggtctga atcacggtta gctccgaagc aaaagccgga taatgttagc
    16381 cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt tttcacctcc
    16441 taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa catgctaatg
    16501 tagccaccaa atcatactac aatttattaa ctgttatcta taatggcgaa aagcgatgct
    16561 gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc cagaagtgag
    16621 taatcttgct tacgccactt ggaagtgacg cattagagat aataactcta atgtttaaac
    16681 tcttttagta aatcacagtg agtgtgtgcg cgagtaagct tttgattttc atcggttaag
    16741 caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac taaaaaagtt
    16801 taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa cgcactcgta
    16861 gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca agtagacaaa
    16921 atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc gaaaatggcc
    16981 gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca ctttgcttct
    17041 gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc tgaagacgac
    17101 acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat cgttccgacc
    17161 actaacccga cttcaacagc tatcttcaaa tcgctgatca gtctgaagac ccgtaacgcc
    17221 attatcttct ccccgcaccc gcgtgcaaaa gatgcgacca acaaagcggc tgatatcgtt
    17281 ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat cgatcaacct
    17341 tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat cctcgcgact
    17401 ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat cggcgtaggc
    17461 gcgggcaaca ctccggttgt tatcgatgaa actgctgata tcaaacgtgc agttgcatct
    17521 gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca gtctgttgtt
    17581 gtcgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg cggctatctg
    17641 ttgcagggta aagagctgaa agctgttcag gacgttatcc tgaaaaacgg tgcgctgaac
    17701 gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt ctctgtacca
    17761 gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga accgttcgca
    17821 catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga agacgcggta
    17881 gaaaaagcag agaaactggt tgctatgggt ggtatcggtc atacctcttg cctgtacact
    17941 gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa aacggctcgt
    18001 atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa cttcaaactc
    18061 gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc tgaaaacgtt
    18121 ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa catgttgtgg
    18181 cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc gctggatgaa
    18241 gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct gttcaacaat
    18301 ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac tgaagtcttc
    18361 ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga actggcaaac
    18421 tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga cgccgcgaag
    18481 atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc gctgcgcttt
    18541 atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc gaaaatgatt
    18601 gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt tgtaactgac
    18661 gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga tatggcgatt
    18721 gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg tggtctggac
    18781 gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt ctctgatggt
    18841 caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta ccacgaaggg
    18901 tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag cgactatcgc gggtatcgcg
    18961 tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg ttcccagttc
    19021 catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg ctacaatgcg
    19081 aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca ggctcgccgt
    19141 cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg tactgctgct
    19201 aagatcgaga aactgctggc atggctggaa acgctgaagg ctgaactggg tattccgaaa
    19261 tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga taaactgtct
    19321 gaagatgcat tcgatgacca gtgcactggc gctaacccgc gttacccgct gatctccgag
    19381 ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaagg tgaaactgca
    19441 gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc cgcttaatca
    19501 gtagcgctgt ctggcaacat aaacggcccc ttctggggcc gtttttttgt ttacccaaag
    19561 caacttttcc ataaaccgac agcattagcc ttcatcatat ttgcgacgat gtataacgcc
    19621 taaacacagg gatattgtac tttacaggtc acaagtcaac gtcggtgctt aagagccctg
    19681 tgaggcgtat agcggcgtta aaaactgccg agaagggtgt atagcccgga agaagtgcgt
    19741 aaaacgaact gacaggataa aagtgccctg ctcaccctgt cagtaaagaa attcttatta
    19801 atcgtggcga tgcctttcct gaatagccgt taatgagccg acttgtaacg cctctttata
    19861 gtgtttacgg catacagaaa cgtatcgttc attaccacca ataaccacct gctcaccttc
    19921 gttataaggt ctgcctgctt gatcaagacg cagcaccatg cttgctttac ggccacaaaa
    19981 acagatggtt tttaattcaa ccagtttgtc ggaccatgcc agtaagtatt ggctgccaat
    20041 aaataattca cctcgaaaat cggtacgtaa accataacaa agtacgggta tatcgagttg
    20101 atcgacaacc tccgataatt catatacttg ttgtctggtt aaaaactggc attcatcaac
    20161 cagtacgcaa tgaattgcct gctgctcatg ttccgcacga atctcatcaa ataatgatga
    20221 attttggtta aataattttg caggcgatga caaacctata cgcgaactga ctttcccggc
    20281 accaaagcga tcatcaattt ctgccgtata tacgacagtg cgcatgccgc gttcctggta
    20341 attgtatgaa gattgcaaca atgctgtaga cttacccgca ttcattgcgg aatagtagaa
    20401 atatagctgt gccatcagcc acaggccctc aatgatatga aataaagttg gctggagttt
    20461 atcataattc gtcagttttt cagctcaaca gtatgcacag aagtggtata tgcgttctcc
    20521 cttacgaagc cttgcataat ccttctgagc tatcattaca actgcctcgc ttgttataag
    20581 cgggtaaatg actgctggta actattcaca atctttaacc tgttgcgcaa gtaatagccc
    20641 tctgttgacc tccaggagat agtgcaatac taagtccctg ctcttattgc gactgttcta
    20701 cttttcatca ttcgcttaat agggaattct cgtaaacacg actaatacag aagactgaaa
    20761 ggtcgtcagc ctacgataat ctccccataa aatgtgacat gaatcaggaa gttttaacct
    20821 cacgtgctgc gaaatcatcg gtacaaatag ggctatatgc cgcgtctttt ctggctaatt
    20881 ttatgaa
//
LOCUS       DAZKIF010000085        20871 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZKIF010000085 DAZKIF010000000
VERSION     DAZKIF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.085"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            148..648
                     /gene="tssB"
                     /locus_tag="ACHOTW_003094"
     CDS             148..648
                     /gene="tssB"
                     /locus_tag="ACHOTW_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP9845153.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            683..907
                     /locus_tag="ACHOTW_003095"
     CDS             683..907
                     /locus_tag="ACHOTW_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845154.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            958..2349
                     /gene="tssC"
                     /locus_tag="ACHOTW_003096"
     CDS             958..2349
                     /gene="tssC"
                     /locus_tag="ACHOTW_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9845155.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2440..2853
                     /gene="tssE"
                     /locus_tag="ACHOTW_003097"
     CDS             2440..2853
                     /gene="tssE"
                     /locus_tag="ACHOTW_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP9845156.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2857..4707
                     /gene="tssF"
                     /locus_tag="ACHOTW_003098"
     CDS             2857..4707
                     /gene="tssF"
                     /locus_tag="ACHOTW_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9845157.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4671..5753
                     /gene="tssG"
                     /locus_tag="ACHOTW_003099"
     CDS             4671..5753
                     /gene="tssG"
                     /locus_tag="ACHOTW_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP9845158.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5778..7058
                     /gene="tagH"
                     /locus_tag="ACHOTW_003100"
     CDS             5778..7058
                     /gene="tagH"
                     /locus_tag="ACHOTW_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFP9845159.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7055..7579
                     /gene="tssJ"
                     /locus_tag="ACHOTW_003101"
     CDS             7055..7579
                     /gene="tssJ"
                     /locus_tag="ACHOTW_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9845160.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7582..8913
                     /gene="tssK"
                     /locus_tag="ACHOTW_003102"
     CDS             7582..8913
                     /gene="tssK"
                     /locus_tag="ACHOTW_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9845161.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8918..9679
                     /gene="icmH"
                     /locus_tag="ACHOTW_003103"
     CDS             8918..9679
                     /gene="icmH"
                     /locus_tag="ACHOTW_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFP9845162.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9688..12459
                     /gene="tssH"
                     /locus_tag="ACHOTW_003104"
                     /gene_synonym="clpV"
     CDS             9688..12459
                     /gene="tssH"
                     /locus_tag="ACHOTW_003104"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP9845163.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQLTAE
                     LDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQD
                     LAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTI
                     NMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLF
                     YQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADFFK
                     PALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVT
                     RAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDE
                     SVTKDETVL"
     gene            12456..13199
                     /gene="vasI"
                     /locus_tag="ACHOTW_003105"
     CDS             12456..13199
                     /gene="vasI"
                     /locus_tag="ACHOTW_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP9845164.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13204..14615
                     /gene="tssA"
                     /locus_tag="ACHOTW_003106"
                     /pseudo
     CDS             13204..14615
                     /gene="tssA"
                     /locus_tag="ACHOTW_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            14752..18186
                     /gene="tssM"
                     /locus_tag="ACHOTW_003107"
     CDS             14752..18186
                     /gene="tssM"
                     /locus_tag="ACHOTW_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFP9845165.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18197..19606
                     /locus_tag="ACHOTW_003108"
     CDS             18197..19606
                     /locus_tag="ACHOTW_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP9845166.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEYKGVLNGEFNLFNTKW"
     gene            19572..20051
                     /locus_tag="ACHOTW_003109"
     CDS             19572..20051
                     /locus_tag="ACHOTW_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9845167.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELFYK
                     IKLTGAKISDIHVSISHIVVDNSVQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
     gene            20072..20293
                     /locus_tag="ACHOTW_003110"
     CDS             20072..20293
                     /locus_tag="ACHOTW_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845168.1"
                     /translation="MANNILHSSTSDSESKNTSTDSTLFNEKFPFQLRQDFASRITED
                     ESDFFKWKKIRELAFVSNHKEWNKYDLLI"
     gene            20372..>20871
                     /locus_tag="ACHOTW_003111"
     CDS             20372..>20871
                     /locus_tag="ACHOTW_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFP9845169.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVIITG
                     WKPITI"
BASE COUNT         5058 a         5640 c         5667 g         4506 t
ORIGIN      
        1 cttatttatt ttgaagcgta aaatgcgggg tgatgaagga gtggtgattt tattttcacc
       61 gccatcaaga cggcatttcc gatatttttt gcggtacgaa tatgccgtgt tcatggcctg
      121 tacgagataa catgacggat cacttttatg agcaaaaaat ttgaaggcag cgtcgcgcct
      181 cgcgaacgta ttaatatatc gtatgtccca aaaacagatg gccagacggc tgaagtggaa
      241 cttccgctta acatgctcgt tgtcggcgat accggaaata cgcaggaaac atcctcactg
      301 gatgaacgtc aggcagtttc cgttaacaag cacaatttcg gtgcagtgat ggctgaagca
      361 gccattggcc tcaattttac cgtccccgcc acgctgaagg gaagcacaac ggacgatgaa
      421 atgaatgtgg cgctgaatat caagtcactg gatgattttt caccagatag cgtggcccgg
      481 caggttccgg aagtgaataa attgctggaa ctgcgtgaag cactgactgc actgaagggg
      541 ccgatgggta accttccggc attccgtacc cagctccagg cattgctgga aaatgaagag
      601 tcccgcgagc agttgctcaa agaaatcggc caggtgagca ataaataacc accactattt
      661 cattaaacag ggttatccag gcatgacggc acacaaaaat atctccggga cataccacct
      721 ctctgttgca gacattctgc aggtggttta ccaggtatgt ttcagcccgt ccgttgaaat
      781 caaccaggat ggtgtggctg cgctgattac aacactggat cgccgtatca gtgatctatt
      841 ggatgagatt atccattttt gtgagtttca gcagagcgcg tcgcactggc agcgcgtact
      901 tcactgacgg cgaaaagccg gtttcagaaa cacagctaaa acagaaggat tttcagtatg
      961 tctttacagg aagaggaact cgtttccagt catgccgggc agcctgagca ggaatcgtcg
     1021 ctgcttgatc aaatcatggc acagacccgt attcagcccg gttcggaagg ttatgacgtt
     1081 gcccgccagg gtgtgacagc ctttattgcc agcatcctgc aatccactgc atctgccgaa
     1141 ccggtcaata aactggccgt ggacagcatg attgcagata ttgatgaacg catcagccgc
     1201 cagatggatg tcatcatcca tgcgcctgcc tttcagcagg tcgagtcgtt ctggcgctca
     1261 ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa cgtgcttcat
     1321 gtcaccaagc aggaacttct ggaagacttt gagtttgcgc cagaaattat tcagtccgga
     1381 ttttataagc acgtttattc gtccggtttc ggacagtttg gcggtgaacc catcgctgcc
     1441 gtgctgggcg cttatgagtt caaaaatacc gcgcctgaca tgaaactgct gcaatacgtc
     1501 agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc ggagtttatg
     1561 gggctgaact cgtggaccga actgcctaat atcaaagatc tttatgccat ctttgaaggt
     1621 ccggcctaca ccaaatggcg cgccctgcgt gactcggaag attcgcgcta tttagggctt
     1681 acggcacccc gcttcctgct tcgccagccc tattcaccaa cagataaccc tgttaagaac
     1741 tttaactatt acgaagatgt cagccagaac cacgaagatt atctgtgggg taatacggcc
     1801 tggatgctgg catgcaatat cgcagacagt tttgccaaat accgctggtg tccaaacatt
     1861 atcggtccgc aaagcggcgg cgcagtgaaa gatctgccgg tgcatctgtt cgaaacgatg
     1921 gggcagattc aggccaaaat cccaaccgag gtgctggtca ccgatcgccg cgaatttgaa
     1981 ctggctgaag agggttttat cacccttacc atgcgtaaag actctgataa tgcagccttt
     2041 ttctctgcaa actcggtaca aaaaccgaag cactttccgg gaaaagatgc agaaaccaac
     2101 tataagctgg gtacgcagct tccttatctc ttcatcatca atcggttagc gcactacatc
     2161 aaggtgttgc agcgtgaaca actggggtca tggaaagaaa ggagcgactt agaaagagag
     2221 cttaatacct ggatccgtca gtatgttgcc gaccaggaaa acccgcctgc agacgtgcgc
     2281 agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg atgtagaagg cgaacccggc
     2341 tggtactagg ttgcgctaag cgtgagacct cattttaaat ttatgggggc aaattttgag
     2401 ctttccctgg ttggccggtt agacagggag taacacacca tgatactgga tcgggaaaga
     2461 accacaggtt ctctttttga gcgtatggaa gcctcttcgg ccagaaaccg gcagggaggc
     2521 agtattcatt cgctcaggca atccattcgc cagaacctaa gaaacattct taatacccgg
     2581 tccggtagtt gccggggtgc gcctgagctg ggcattgatg aaccggaagg ggctgaaaac
     2641 ttccgcgaat ccatgagtcg ggccatcgag caatgtatag aacgctatga accccggatc
     2701 tcacacgctg aagtgcaggc agtggtttca tccgcctcca gcccgctgga tatgacgttt
     2761 catattacag cctgggtgac atttaacgaa acgcatgagg tgcttgaatt tgatatggca
     2821 ccgaatggca gccagcatta ccgggtggac tgacaaatgg aatttgaaga acgctatttt
     2881 cgggaagaac tcgattacct gcgccagctt agcaagctgc tggcaacgga aaaaccccat
     2941 ctggcccgct tcctggccga aaaagatgcg gatccggata ttgaacgcct gctggaaggg
     3001 gtggcttttc ttaccggcaa tctccgccag aaaattgagg atgaattccc agaactgacg
     3061 cacgggctta ttaagatgct atggcctaat tacctgcgtc cggttccggc aatgaccctt
     3121 attgaatata cgccggatat ggataagtct tctgtaccgg tgttaatccc ccgtaatgag
     3181 cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag tgctgccctc tgatgctaaa
     3241 aaggaggagc cgcctccctg taccttcacc ctctgccggg atatctggct gctgcccgtt
     3301 cgcctggagc agattgaaaa ccgcagtacg acccgtaatg gtgttatcaa catcaccttt
     3361 tcggtcgcac cgggaacgga cttccgcacg ctggatctga acaaacttcg cttctggctc
     3421 ggcaatgacg acaactatac ccgtgaccag ctttatttat ggttctgcga atacttgcag
     3481 ggtgccgacc tgactgtggg tgaacagcat attcgcctgc ctgagtttat gctaaaagct
     3541 gtcggttttg agccgcagga tgccatgctg ccctggccga aaaacgtcca cagcggctac
     3601 cggatccttc aggagtattt ctgttacccc gatgcgtttc tcttttttga tctttgtggt
     3661 tgtccggctt tgcctgacgg attgcaggcg gaattcttta ccctgcaact gcgtttttcg
     3721 cgccctttgc ccgtggacat ccggctgcgc cgcgattccc tgcgcctgta ttgcgcacct
     3781 gccattaatt tatttatcca ccatgcagaa gccatcacgc tggacaaccg gcgggcagac
     3841 tatccgctgg ttcccagccg ccattaccca caacattacg atgtattttc cgttaacagt
     3901 gtggtgagcc aggtccagga tatgttcagg aaaaaagatc tggggcgtcc tgtttcgacg
     3961 caggccgcgc gccagtggcc agcctttgaa agtttcagcc atcagatgga atacagccgg
     4021 aagcgggagg tggtgtactg gcatcaccgg accaaaacat ccctgttcca tcgcggcttt
     4081 gatcataccc ttgcctttat acatgctgat ggcagttatc cgtcagacga atctctgctc
     4141 agtaatgaag tggtttcggt atcgctgacc tgtaccaacc gtgagcttcc gtcacaaatt
     4201 cgttccggcg atatcaccgg cacaaccggt aaaaatgcgg ctgttgcttc ctttcgcaac
     4261 attacccgcc cgacgcaacc actctggccg gtcattgatg gcagcctgca ctggtccctg
     4321 ctctccgcca tgaacctgaa ttatctgtca ttactggata cggacgcgct gaagcaggtc
     4381 atcgccaact ttgatcgcca cgcaatccac catccgcaga cggcgcggct gtcacaacaa
     4441 aagctggatg ccattgagcg tctggagacc cgccccgttg atcgcctgtt tacgggtatt
     4501 cccgtccggg gactggcctc cacgctgtat ctgcacccgg agccgtttgt ctgtgaaggg
     4561 gaaatgtatc tgctcggtac ggtgctttcg cattttctgt cgctgtacgc cagcgttaac
     4621 tcattccaca tgctgaccgt tgtgaacaca gaaagccagg agacatggaa atggacggaa
     4681 agaatcgggc agcatcctct tatctgagtc cgggaaatcc cccggcagat aaagagcaga
     4741 atgatccgct ggcacaggtt ttccataatg cctgctctta caattttttt gcgatggcgg
     4801 agctgttgca ccgcctggca aagggtgaaa aggggacgcc agaattatcc ctgcgcgacg
     4861 atccggcaca ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc ccgtgcagtg
     4921 atatcagtgc gctgaaacgg gatacgtcag gggcattcag gatgaccact acctttatgg
     4981 ggttacaggg gagccagtcg cctctccccg gttattacct cgatcacctg gcctggaaag
     5041 cggtacatga acaaagtccg gtaggtgatt ttctggatat gttcagccac cgcctgaccc
     5101 agtttgtctg gcatatctgg cgtaagtacc gctatcacat cagttttcgt aacggcggtg
     5161 tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc cagcttcgcg
     5221 ataagctggc gattaatcac agcaaaatgc tggcgtattc cgggatcctg gcaaatccgg
     5281 gacgatcccc ggaaattatc tgtggcctgg tgtcccactg ctttgatttg tcagaagtga
     5341 cgctgcaaaa ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa aacagcctgg
     5401 ggagctattc tcttaaaaat ggcgaaaaac tggcgggaag atcggtactg ggcaactttg
     5461 ttctgggtac acgagtaccc gatctcagcg gaaaattcca gctcagtatt accagcctga
     5521 cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa cttcctgcca ctgacgacgt
     5581 tcgtgtcctt catcctgcgc gatcagcttg cctgggactt acatctgggg ctggcgccgg
     5641 agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc tggaccagct
     5701 tcctcggcac accggaagaa cgaccctcag tcaccatcag ggtacggtcg taaattatga
     5761 taatcacgga gcagaatatg ccggaagaga aactacagac gctttcattg caggtcatta
     5821 acggcagtga gctggaaagc gggcgggcgg cgcgctgtct gttcacacag cagggaaatg
     5881 tcggccatgc ccccgaatgc cactggtcgg tacaggatcg tcagcagagc attccggccc
     5941 aggcttttac cgttatcctg cacgatggca cattttgtct acgcccgcag acggcacaac
     6001 tgtggctgaa tcaggcaaaa gtcacagcaa catcagacct gatacagttg cgccagggcg
     6061 atgagatcca gatcggacgg ctgatggtga gggttcatct gaaccgggga gatattcccc
     6121 attacgatga ggaaatggcc actcccgaaa ccatcgttac caatcgcgat atgctcacgg
     6181 ataccctgct atcaacggag ggtgcgccac actatccggg aatgactcac cggcaccagc
     6241 ttgcagacac cgtggtaaat ggtttttctg ccgatccact ccaggcactt cagtccgaaa
     6301 gcctgattac cacgggcgat ccgctttcag gcattgcggc tgtccggcca tcggcaccgc
     6361 tgtccgatcc ggcaagtaat ggggggatca atactccgtt tatggatctg ccgcccattt
     6421 atgccagccc tggcgatcat aatgatgaca tctctgcggc agaaatggcg caacgccacc
     6481 ttgcggtcac ccccttactg cgcggtcttg gcggctcgct taccgtgagc aattccgacg
     6541 atgcggatga ttttctggag gaggccggac gaacgttaca ggccgcaata aaaggtctgc
     6601 tcgatttgca gcagcagcgt aacagcctct cagacaaaca tttgcgcccg ctggaagata
     6661 acccgctgcg cctgaacatg gattacgcca ccgcgctcga cgtgatgttt gccgaaggta
     6721 aaagcccggt acatctggcg gctcccgccg ccgtcagtga aagcctgcgc aatgtccgcc
     6781 accacgaaga agctaaccgg gcggcgattg tggagtcgct tcgtgtcctg ctggatgctt
     6841 tctcaccaca aaatctgctg cgccgctttg tgcagtaccg ccgcagccat gaactgcgcc
     6901 agccgctgga tgatgccgga gcatggcaaa tgtacagcca ttattacgaa gaactggcct
     6961 ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt ctacgcccag gtctatgacc
     7021 gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt ttctggcctg
     7081 cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca gcgaggtgat
     7141 caaaaacccg gatatccagg tcgggagcct gaaggagcaa ccttcggaaa taaccgtcac
     7201 cctgctgact gaaccggaca ccaataccaa tgcggagggt gaatcagccg ccgtggatgt
     7261 gcaactggtg tacctgactg acgactcaaa attgcaggca gccgactatg accagatagc
     7321 cagtacgccg ctaccggatg tgctgggtaa aaactatatc gaccaccagg atttcaacct
     7381 gttgccggac accatcaaaa ccctgccgcc gataaagctg gatgagaaaa cgcagtttat
     7441 tggtgtcgtg gcctatttct ccgatgacca ggccactgaa tggaaacaaa tcgagacggt
     7501 ggaaggaacc ggccaccatt accgcctgct ggtgcatgta cgtcaaagca gcatcgaaat
     7561 gaaaaaagag gatgaataat tatggcaacc acccgcaaca aggtgatgtg gcaggaaggg
     7621 atgctgatgc gcccacacca tttccagcaa cagcagcgtt acaacgacta cctggataac
     7681 cagcgtttcc gggccatgaa tgatttatcc tggggattta ccgaactcac cctcaacaat
     7741 gaactgctgg cgcagggtaa gatcatgatt gacagcgcgt caggcacact gcctgacggc
     7801 accgtctttt ctatccccga ccaggacgca ctgcccgatc cgctgcaccc gcaaaatttt
     7861 ccggacgaaa gaagccgcaa tatttacctc gctctgccgg tcgccagtga tgtgagaaat
     7921 gaaatcagcg acgggcggcg aatcgggcgt taccggctga attatgccga tgtccgggat
     7981 ctgcattcag aagaaggcga cgcgcgaaca ctgacgctgg gacaactgac gccgcgcatt
     8041 atgagcggtg cagaagatat gagtgcctat attacgctcc cgctttgccg tatcagcgat
     8101 cgccatgccg acggctccct gacgctggat gacgatttta tcccctcctg ccagaatatt
     8161 caggtcagta agaaactgcg tgtttatctc aaagaggtac agggggccat tggcggacgg
     8221 gcaagcgatc tggcaaaccg tattggctct ccggcgcaga gcggcatcgc ggatgtggcg
     8281 gaatttatga tgttgcagtt gcttaaccgt aaccagaccc ggtttaccca tcgcgcccgc
     8341 cgatcccagc tccacccgga agatttctac cttgatcttg ccgagttgct gggtgaactg
     8401 atgaccttta cagagccgtc acgcctgccc tgcccgcttg atgtgtatga tcatcatgac
     8461 ctgaccaaaa catttaaaac actgttaccg gaagtcaaac gggcgctgca taccgtactg
     8521 tcgccaagag cggtcaatct gccgctgcat ctgcgtgacg gcatctggca ggccgatgtt
     8581 catgactcgg aactgctaca gtctgccacc tttgtactgg ccgtggcagc aaatatgcct
     8641 gtcgatcaga tccagcgtca gtttatccag cagtcgaaaa tttcctcgcc ggaaaaaatc
     8701 cgcaatatgg tcagtgtgca gatcccgggt attcctctgc gcgcactgat ggtggccccc
     8761 cgccagcttc cctaccattc cgggttcagc tatttcgaac tcgacaagag cggccaggcc
     8821 tggacagaaa tggctgccgc cggggccgtt gcactgcatg tatccggcag tttcccggat
     8881 ctgaacatgc aactgtgggc gataagaggg taagacgatg gatgaggggt cactgtctct
     8941 gccaccattt accggatacg acgaaaaaag cctgcgcgac taccatctcg cgcttcatgg
     9001 caacagcctg aatccgatga tcgacgcggc gacgccgcta ctcggtatgg taatgcgcct
     9061 ttccaccatg aacagccaga cgatgccgga gcatcttttt gctcaggtgg tgacagatgt
     9121 tcaggccgtg gagcaactgc ttcaggaaca gggctacgaa ccgggcgtga ttatctcgtt
     9181 tcgctacatc ctctgcacgt ttattgatga agcggcgctc ggtaacggct ggtcgaacaa
     9241 gaacgagtgg atcaagcagt cattgctggt ccatttccac aacgaagcct ggggtggcga
     9301 gaaagttttc atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc aggatctgct
     9361 ggaatttctc tggctctgtt tttctctggg atttcgcggg cgctacaaag tggcagtaca
     9421 ggatcagggg gaatttgagc agatctaccg ccgtctgtac cacgttctgc ataaactgcg
     9481 cggcgatgcc cctttcccgc tgttacacca ggacaaaaaa acccaaggcg ggcgttatca
     9541 gctcatcagt cgcctgactg ttaaacatat tttttgtggt ggcgttgtcg tgctggcgtt
     9601 gttttacctg ttctatctgt tgcggctgga cagccagacg caggacatcc tgcaccagct
     9661 aaacaaatta cttcgataag gacgtttatg atccagattg atcttcccac gctggtaaaa
     9721 cggctgaacc tgttctcccg ccaggcgctg gagatggccg cctcagaatg tatgagccag
     9781 caggcagcgg aaattaccgt cagccatgtg cttattcaga tgctcgccat gccacgcagt
     9841 gacctgcggg ttattacccg ccagggcgat attggcatgg aagagttgcg ccaggcgttg
     9901 acggtagaga actacacaac cgcccgttct gcggacagct acccggcgtt ttccccgatg
     9961 ctggttgagt ggcttaaaga gggctggctg ctggcgtcgg ctgagatgca gcacagcgaa
    10021 ctgcgcggcg gcgtgttgct gctggccttg ctgcattcgc cgctgcgtta tataccgcct
    10081 gctgccgccc ggctgttgac cggcattaac cgtgaccgtc tgcaacagga ctttgtgcag
    10141 tggacacagg agtcggcgga atcagtcgtg ccggatgcag acggtaaagg cgcaggcaca
    10201 ctgacggacg cctctgacac cctgcttgcc cgctacgcca aaaacatgac agaagacgcc
    10261 cgtaacggca ggctagcccc agtactgtgc cgcgaccacg aaatcgacct gatgatcgac
    10321 attctctgcc gccgccgtaa aaacaacccg gtggtggtgg gcgaagcagg cgtgggtaaa
    10381 agcgcgctga ttgaagggct ggcgctgcgc atcgtggcag gccaggtgcc ggacaaactg
    10441 aaaaacaccg atatcatgac ccttgacctg ggcgcattgc aggccggggc atcggtgaag
    10501 ggtgagtttg aaaaacgctt caaggggctg atggcggagg tcatttcctc tccggtgccg
    10561 gtcattctgt ttatcgacga agcgcatacc ctgattggcg cgggcaacca gcagggcggg
    10621 ctggatatct ccaacctgct gaaaccggcg ctggcgcgtg gcgaactgaa aaccatcgcc
    10681 gccaccacct ggagcgagta caaaaaatac ttcgagaaag acgccgccct gtcgcgccgc
    10741 ttccagttag tgaaggtcag cgaacccaac gctgccgaag ccaccattat tctgcgtggt
    10801 ctgtcggcgg tctatgaacg gtctcacggc gtgctgattg acgatgatgc cttgcaggcc
    10861 gcagcaacgc tgagcgaacg ttatctctcc gggcgtcagt taccggacaa agcgattgat
    10921 gtgctggata ccgcctgcgc ccgtgtggcc atcaacctgt cgtcgccgcc gaagcaaatc
    10981 tcggcgctga ccactctgag ccaccagcag gaggcggaaa ttcgccagct tgagcgcgag
    11041 cttcgcatcg gactgcgtac cgacacatca cggatgaccg aggtgctggt gcagtatgat
    11101 gaaacgctga cggcgctgga tgaactggaa gcggcttggc accagcagca gacgctggtc
    11161 cgggagatta tcgcgctgcg ccagcagtta ctgggcgtgg cagaggacga tgcggcgccg
    11221 ttgccggacg cagatactgt ggaggatacg cagccagagt cagagtcaga gtcagaacag
    11281 gataataccg gtgccgtacc ggctgatgag accgacagag aacagccgga agagaccgct
    11341 gaaacagttt ccccggtaca gcggctggcc cagctcactg ccgaactgga cgccctgcat
    11401 aacgaccggt tgctggtctc cccgcacgtc gataaaaaac agattgcggc ggtgattgcc
    11461 gaatggaccg gcgtgccgct caaccgcctg tcgcagaatg aaatgtcggt catcaccgac
    11521 ctgccgaaat ggctgggcga caccatcaaa ggccaggacc tggcgattgc cagcctgcat
    11581 aagcatctac tgaccgcacg cgccgacctg cgtcgtccgg gacgcccgct cggtgcgttc
    11641 ctgctcgctg gccccagcgg cgtgggtaaa accgaaaccg tcctgcaact ggcagagctg
    11701 ctctacggcg gtcgccagta cctgaccacc atcaatatgt ccgagttcca ggagaaacac
    11761 accgtttcgc ggctgattgg ctcccctccg ggctatgtcg gctatggcga aggcggcgtg
    11821 ctgactgaag ccatccgcca gaaaccctac tcggtggtgc tgctcgatga agtggaaaaa
    11881 gcgcacccgg atgtcctcaa cctgttctac caggcgtttg acaagggcga gatggccgac
    11941 ggcgaaggcc gcctgattga ctgtaaaaac atcgtcttct tcctcacctc caacctcggt
    12001 taccaggtga ttgtcgaaca cgcggatgac ccggaaacca tgcaggaagc cctctatccg
    12061 gtgctggcgg acttctttaa acctgccctg ctggcgcgaa tggaggtggt gccgtacctg
    12121 ccgctgtcga aagagacgct cgccaccatt atcgccggga aactggcccg tctggataac
    12181 gtgctgcgca gtcgctttgg tgcagaagtg gtcattgaac cggaagtgac ggacgaaatc
    12241 atgagccgcg tcacccgcgc ggaaaacggc gcgaggatgc tggaatctgt catcgatggc
    12301 gacatgctac cgccgctctc gctgctgctg ttgcagaaaa tggcggctaa cacggcgatt
    12361 gcccggattc ggttgtcggc agtggacggc gcatttacgg cagacgtgga agatgctcag
    12421 aacgacgagt ccgtcacaaa ggatgaaacg gttttatgag caggttgcgc gttttccggc
    12481 agacaggatg gtttatcgcg ggcctgatga cgggcttacc cgccactgcc gcgcccgcag
    12541 aagcatcgtc aatggccggt gtggcggtcg ccgtcgccac cacgaccccg ccggatgcga
    12601 cagccacgtt acaggccatg cagtcctgtc ggcgggagtc cgcggcgctg gagcggcttg
    12661 actgctatga ccacctgctg gcaccgctgt ccccgtcagg gtttgacggg gcgctggtca
    12721 aagccggttt tgtgggcgag gcctggaccc gcgccacaga gcaggaaaaa cgccgcgagg
    12781 gcaacaccac agaactgctg gtgacgcagg tgccggggga gcgcccgacg gtggtgataa
    12841 ccaccccggc tatcggccat gttccgccgc gcccggtgct gatgttcagt tgcgtggaca
    12901 atatcacccg gatgcaggtg gcgctgatgc accctctgga tgtccatgat atcgccgtca
    12961 ctctgaatgc cgacagccgg gcattgcgca gccactggtt tgtgcgcgag aacggcaccc
    13021 tgctggagtc cagccggggg ctgtccggga ttgatgaaat taagcaactg ttcggcgcaa
    13081 aaacgctgac agtggatacc ggcgcagata acgccgcagg aaagctgacc tttaacattg
    13141 acgggctggc aagggcgatt gcccccctgc gcgatgcctg ccactgggcg ggagaataaa
    13201 aatatggcaa tggacttacg cgatccgaat gtctggatat cgcacctgct ggaaaacctg
    13261 ccggaagaaa aactggcatc ggcgctgaaa gatgacaacc cggactggga gtatatcgac
    13321 ggcgaaatcg tcaagctggg gtctctggcc cacgctcagc ttgatattcc cgaactacaa
    13381 cgcagggggc tacagcttct ggcttctgaa agcaaagact tcaggctact ggcacacctg
    13441 ctcagaaccc tgcaacatgc cggtgatcca ctgctggcac tgcacctgct aacgctatac
    13501 gtggaacatt actggactgt ggccgcgccg cagaatatgg cgcataaaaa acgctttgcc
    13561 agccagatca ttaaacgttt tgaaacggta ttgaaggctt ttcacaaaac gctgccacaa
    13621 cgcagcgcga tactctgctg ggtgagctgg cgaaactggc gcagtgctgg cagtcacata
    13681 acgtcccgga actggcacag gctaccgatg atctttttgc cctgtaccag cgtacgttta
    13741 atcgtgcggc tcctgctccg gtccccactc cggcggcctc cggtagttca ccacaaacca
    13801 ccgtcacgtc tgaaagcggc gtgacgcaac ccagtgctcc ggctccccaa atcgccatcg
    13861 acagtcacga cgacaaagcc tggcgcgaca cgctgttaaa agtggcggct attttatgtg
    13921 aacgccagcc ggactcgccg cagggctatc gcctgcgccg ccatgccctg tggcaatcca
    13981 tcaccagtac accccaggcg gaaagcgatg gacgtacccc actggctgcg gtctctgccg
    14041 atatggtggc ggattaccag tcccggcttg ccagcgcgga tatggcgctg tggcaacagg
    14101 ttgagaaaag cgtattgctg gctccttact ggctggacgg tcactgtctt tctgcacaga
    14161 cggcactgcg tctgggttac aaacaggtgg cagacaccat ccgcgatgag gtcatccgct
    14221 tccttgagcg tctgccccag cttaccgggc tgctgtttaa tgaccgcaca ccgtttctca
    14281 gtgagcagac gaaacaatgg ctggctgctt cgcccgacgg caaagttgca ccggttgcgc
    14341 aaatcggtga ggaatcgcag gcagccagag cctgttttgc tgggcagggt ctggaggcgg
    14401 cgctgcgata tctggacatg ctacccgaag gcgatccccg cgatcagttt caccgccagt
    14461 accttgccgc acagttgacg gaggaggcgg ggctgataca gcttgcgcag caacagtacc
    14521 ggatgttgtt gatgataggg agtcagatga tggtgtctga ctgggagcca tcattactta
    14581 cgcagcttga acaaaaattc acggcagaac aataaaagag aacaggagtt tttgtgttca
    14641 gattaccaac accccgacta ctcagcggac tcaaatcagc cctgcgaccg gcgatgcccc
    14701 ggtttaaagt ctccgctttc tggctgctga tactggcgtg gatttttctg cttgctgata
    14761 ctggcgtgga tttttctgct ggtatggatc tggtggaaag gcccgacgtg gacgctgtat
    14821 gaagaacagt ggctcaaacc actggcgaac cgctggctgg caacggcggc gtgggggatt
    14881 attgccctgg tgtggcttac cgtccgggta atgaagcgcc tgcaactgct tgaaaaacag
    14941 caaaaacagc agcgcgaaga ggccattgat ccgctcagtg tggagctgaa cgcccagcag
    15001 cgttaccttg accgctggct gctgcgcctg caacgccatc tcgacaaccg ccgtttcctg
    15061 tggcagttac cgtggtatat ggtcatcggc ccggcgggca gtggcaaaac gacactgctg
    15121 cgcgaggggt ttccgtcaga cattatttat gccccggagg gcgcgcgcgg cacagaacaa
    15181 cgcctgtacc ttacgcccca tgtcggtaaa caggcggtga tctttgatat cgacggcaca
    15241 ctctgcgctc ccgctgatgc ggatatcctg catcgccgtc tgtgggaaca tgccctcggc
    15301 tggctgaaag aaaagcgcgc gcgccagccg ctgaacggga ttattctgac actcgattta
    15361 cccgatctgc tgaccgcaga caaacgccac cgcgagcatc tgttacagac gctgcgcagc
    15421 cgcttacagg atatacgcca gcatcttcac tgccagttac cggtttacgt ggtactgacc
    15481 cggcttgatt tattacaggg ttttgccgcc ctgttccagt ccctgaacag acaggatcgc
    15541 gatgcgattc tgggcgtcac gttcacccgc cgtgcccatg aaaatgatga ctggcgaaca
    15601 gagttgaatg ctttctggca gacatgggtg gatcgaatga atctggcgtt gccggatctg
    15661 atggtcaccc agactcacac ccgcgcgtct ttattcagtt tttcccgcca gatgcaggga
    15721 agccgtgaac cgctggtgtc actgcttgag ggtctgcttg acggtgaaaa tatgaacgtg
    15781 atgctgcgtg gtgtctatct cacctcttcg cttcagcgcg ggcagatgga tgatatattc
    15841 acccagtctg ccgcccgcca gtaccgactg ggcaataacc cactggcgtc ctggcctctg
    15901 gtggacaccg cgccttattt cacccgcagc ttgttcccgc aggcattact cgcagagcct
    15961 aatctggcaa cggagagccg cgcctggctg atgcgttccc gtcgccgcct gacggttttc
    16021 tctgccaccg gcggcgtggc ggcactgctg ctcatcaccg gctggcatca ctattacaac
    16081 ggtaactatc agtccggcat caccgtgctt aagcaggcca aagcctttat ggatgtgccg
    16141 cctccacagg gggaagatga ctacggcaac ctgcaactgc cgctgctgaa cccggtacgc
    16201 gatgccacac tggcctatgg cgactggggc gaccgcagcc gtctggccga tatgggactg
    16261 taccagggac gacgtatcgg accttatgtg gaacagacct atctgcaact gctggagcaa
    16321 cgttacctgc cctcgctgtt taacgggctg gttaaagcga tgaatgccgc gccgccggag
    16381 agtgaagaaa aactcgcggt gctgcgcgtg atgcgaatgc tggaggacaa aagcggacgt
    16441 aacaatgagg tggtgaagca gtatatggca aaacgctgga gcgaaaagtt ccacggccag
    16501 cgcgatatcc aggcacaact gatgtcccat cttgactacg cgctggctca tactgactgg
    16561 cacgcagagc gtcaggcggg cgacggtgac gccatcagcc gctggacgcc atatgacaag
    16621 cccgtggtat cagcacagaa agaactgagc aaactgcctg tctaccagcg ggtttaccag
    16681 agcctgaaaa cgcgggcgct gggcgttctt cctgccgacc tcaatctgcg tgaccaggta
    16741 gggccaacct ttgaccaggt gtttacatct gccgatgaca acaaactggt tgttccacag
    16801 tttcttaccc gttacggcct gcaaagctat tttgtaaaac agcgcgatga actggttgaa
    16861 ctgacggcga tggattcctg ggtacttaac cttacccgca gcgtgaaata cagtgacgcc
    16921 gaccgcgcgg aaatccagcg ccagctcacc gagcagtata tcagcgacta caccgccacc
    16981 tggcgggccg ggatggacaa tctgaatatc cgcaattttg agtccatcgg acaactgacc
    17041 ggggcgctgg agcaggttat cagcggcgac cagcctttgc agcgggcgct gaccgtgctg
    17101 cgtgacaaca cacagccagg cgtcttttct gaaaaactct ctgccaaaga acgggatgag
    17161 gccctggcag agccggacta ccagttactc acccgtctcg ggcatgagtt cgccccggaa
    17221 aacattaccc tggcagtaca gaaagacaaa gaaagcacga tgcaggccgt gtatcagcaa
    17281 ctcaccgagt tgcaccgcta cctgctggca atccagaacg cgcctgtacc agggaaatcg
    17341 gcgctgaaag ccgtgcagtt acggcttgat cagaacagca gcgatccgat attcgccacc
    17401 cgccagatgg caaaaacgct gcctgctccg ctcaaccgct gggttggcag actgactgac
    17461 caggcctggc atgtggtgat ggtggaggct gtacattata tggaagtgga ctggcgcgac
    17521 agcgtggtga aaccgtttaa cgagcaactg gcaaataact atccgtttaa tccgcgttct
    17581 gcacaggatg cctcactgga tgccttcgaa cgcttcttta aaccggatgg catactggat
    17641 accttctacc agcagaacct gaagctgttt atcgataatg acctgagtct ggaggatggc
    17701 gataacaacg tcattattcg cgaagatatt attgcgcaac tggaaactgc gcagaaaatc
    17761 cgtgacatct tcttcagcaa acagaacggt ctgggaacat cctttgccgt ggaaacggta
    17821 tcgctttcag gcaataaacg ccgcagtgta ctgaaccttg acggtcagtt agtcgattac
    17881 agccagggcc gtaactatac cgcccatctg gtctggccta acaacatgcg cgaaggcaac
    17941 gaaagtaagc tgacgctcat cggcaccagc ggcaacgcgc cgcgcagtat cagcttcagc
    18001 gggccgtggg cgcagttccg cctgttcggg gccggacaac tgaccggagt acaggatggc
    18061 aactttaccg tgcgctttag cgtggacggt ggcgcgatga cctaccgtgt gcataccgac
    18121 acggaagata acccgttcag cggtgggttg ttcagccagt ttggtctgtc agacacactg
    18181 tactgaggag gcagggatga atagtaacgt actgacacaa actatcgtta ccggcagtga
    18241 cccgcgcggg ctgccggaat tcagcgccat ccgcgaggaa ataaacaaag ccagccaccc
    18301 gtcacagcct gagctgaact ggaaactggt ggagtcgctg gcgctggcga tttttaaagc
    18361 caacggtgtg gatttacaca ccgccaccta ctatacgctt gcccggacac ggacacaggg
    18421 actggcggga ttctgcgaag gtgcggaact gctggcggca atggtaagcc acgactggga
    18481 taagttctgg ccgcagggcg gcccggcgcg tactgaaatg ctggactggt ttaactcccg
    18541 aaccggcaat attctgcgtc agcaaatctc ctttgcggaa tccgacctac cactgatcta
    18601 ccgcacagag cgggcattgc agcttatctg cgataagctc cagcaggtgg aactgaagcg
    18661 cgttccgcgc gtggagaatc tgctctattt tatgcagaac acgcgtaaac ggcttgaacc
    18721 ccagctgaag agtaacactg agaacgccgc acagaccacg gtcagaacgc tgatttatgc
    18781 cccggaaaca caggcatctt ccacaccaga agcggtagtg cctcccctgc ccggcctgcc
    18841 tgagatgaaa gtggaagtgc gcagtctgac agagaatccc ccacaggcca gtgtgataaa
    18901 gcaaggcagt acggtaagag ggtttatcgc agggatcgcc tgttcagtgg ctgtcgcctc
    18961 agcattgtgg tggtggcagg tctatccggt gcagcagcaa ctgttacagg ttaacgacac
    19021 cgctcagggc gcagcaacgg tgtggatggc ctcacctgaa ctcgaaaact atgagcgcag
    19081 gctgcaacaa cttcttgata cctccccggt acagccgctg gaaaccggga tgcagatgat
    19141 gcgtgttgcc gacagtcgct ggccggaaag cctgcaacag caacaggcct cgacacaatg
    19201 gaatgaggca ctcaaaaccc gcgcacagag tagcccgcag ttgcgtggct ggttgcagac
    19261 ccgccaggac ttacatgctt ttgcagatct agtgatgcag cgcgagaaag agggactaac
    19321 cctttcctat atcaaaaatg tcatctggca ggcggagcgg ggactggggc aggaaacacc
    19381 cgttgagtct ctgttgacgc agtaccagga tgcccgtgcg cagaagcaga atacagatgc
    19441 gctggaaaaa caaattaatg agcgactcga aggcgtgtta agccgctggc tgctgctgaa
    19501 gaataatacg ataccgacga taaaaaaagc attgaatttc aataacattc atgaatacaa
    19561 aggagtttta aatggcgaat ttaatttatt taacactaaa tggtgataat caagggctca
    19621 tttcaagtgg ttgttcatca caaccatcca ttggaaataa agctcaaaca gcacacattg
    19681 atcaaattat gatttatgaa ttcatgcatg gattaaatcg agatcaaaac gtcaaccatc
    19741 atcatttgac aataaaaaaa cctatagata aagcatcacc cttattaggc aaagcaattt
    19801 gcgacaatga attattgaca tgtgattttt ccttttatcg tacaaataaa tttggaatta
    19861 atgaactgtt ctataaaatc aaattaactg gagcaaagat aagcgacatt catgtctcta
    19921 tatcacatat tgttgttgat aacagcgttc aacccgaaga gtcagtttct ttcagttatg
    19981 agtcaattat atgggagcat tgttccgctg gcactagtgc atacagttta tgggaagatc
    20041 gtctttttta aaaacatatg ggattattat aatggctaac aatattttac actcttcaac
    20101 atcagacagt gaaagcaaaa atacaagcac tgacagtacg ttatttaatg aaaaattccc
    20161 atttcaacta agacaagatt tcgcatcaag aataacagaa gatgaaagtg atttttttaa
    20221 atggaaaaaa ataagagagt tggcatttgt gagcaatcat aaagaatgga ataaatatga
    20281 cttattaatt taaaaatctg ttaatgaaat caacattcat ttgtccagca ctccatactt
    20341 tcagccgttg gattggtata taataaaagc catgctctgg actgaaaacg atgcggaaaa
    20401 tacttctcag tggaatggtt atccattaca aattggtcgt tttcggaaag ataaagcgat
    20461 gccagcttta atctctggag aaaaatctac agcccttgtt accccaccac aatggagaaa
    20521 caaagcattc aatgggttaa aagatccaga aagaaattac tgggcaaaag aacaaataac
    20581 tggctctcca gaagagaata tcaaagcagc aattacttac ttaatgatga aactttctaa
    20641 tacaaaggaa gaatcaacta tcgatcaata tgactcaact ctatatagcg ccattgtcca
    20701 aaaaggcgat ttggctgaca acatcagaaa agagagaaaa acgaccatac ctaacttaac
    20761 gaaaaacaat cctggaaaaa atctcgataa aatacacccg ggagatattt tatattatca
    20821 aaaagcatcc atgaaagtaa taattactgg atggaaacct atcacaataa a
//
LOCUS       DAZKIF010000086        20816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZKIF010000086 DAZKIF010000000
VERSION     DAZKIF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.086"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..142
                     /locus_tag="ACHOTW_003112"
     CDS             <1..142
                     /locus_tag="ACHOTW_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP9845170.1"
                     /translation="GKNETITTPRLSFRFLNVSPTVERQLQRIIFSLEREAREKADKV
                     RD"
     gene            complement(144..755)
                     /gene="emtA"
                     /locus_tag="ACHOTW_003113"
     CDS             complement(144..755)
                     /gene="emtA"
                     /locus_tag="ACHOTW_003113"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP9845171.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            855..1769
                     /gene="ldcA"
                     /locus_tag="ACHOTW_003114"
     CDS             855..1769
                     /gene="ldcA"
                     /locus_tag="ACHOTW_003114"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP9845172.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            1865..3601
                     /gene="cvrA"
                     /locus_tag="ACHOTW_003115"
     CDS             1865..3601
                     /gene="cvrA"
                     /locus_tag="ACHOTW_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP9845173.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(3993..5063)
                     /gene="dadX"
                     /locus_tag="ACHOTW_003116"
     CDS             complement(3993..5063)
                     /gene="dadX"
                     /locus_tag="ACHOTW_003116"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP9845174.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(5073..6371)
                     /gene="dadA"
                     /locus_tag="ACHOTW_003117"
     CDS             complement(5073..6371)
                     /gene="dadA"
                     /locus_tag="ACHOTW_003117"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP9845175.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            6734..8266
                     /gene="ycgB"
                     /locus_tag="ACHOTW_003118"
     CDS             6734..8266
                     /gene="ycgB"
                     /locus_tag="ACHOTW_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP9845176.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(8318..9037)
                     /gene="fadR"
                     /locus_tag="ACHOTW_003119"
     CDS             complement(8318..9037)
                     /gene="fadR"
                     /locus_tag="ACHOTW_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP9845177.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            9259..10800
                     /gene="nhaB"
                     /locus_tag="ACHOTW_003120"
     CDS             9259..10800
                     /gene="nhaB"
                     /locus_tag="ACHOTW_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP9845178.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            10946..11476
                     /gene="dsbB"
                     /locus_tag="ACHOTW_003121"
     CDS             10946..11476
                     /gene="dsbB"
                     /locus_tag="ACHOTW_003121"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP9845179.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(11522..12790)
                     /gene="umuC"
                     /locus_tag="ACHOTW_003122"
     CDS             complement(11522..12790)
                     /gene="umuC"
                     /locus_tag="ACHOTW_003122"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFP9845180.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(12790..13209)
                     /gene="umuD"
                     /locus_tag="ACHOTW_003123"
     CDS             complement(12790..13209)
                     /gene="umuD"
                     /locus_tag="ACHOTW_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP9845181.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            13582..14493
                     /gene="hlyE"
                     /locus_tag="ACHOTW_003124"
     CDS             13582..14493
                     /gene="hlyE"
                     /locus_tag="ACHOTW_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFP9845182.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGNIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AANKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(14700..15146)
                     /gene="ycgN"
                     /locus_tag="ACHOTW_003125"
     CDS             complement(14700..15146)
                     /gene="ycgN"
                     /locus_tag="ACHOTW_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP9845183.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(15238..15897)
                     /gene="ycgM"
                     /locus_tag="ACHOTW_003126"
     CDS             complement(15238..15897)
                     /gene="ycgM"
                     /locus_tag="ACHOTW_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP9845184.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(15969..16262)
                     /locus_tag="ACHOTW_003127"
     CDS             complement(15969..16262)
                     /locus_tag="ACHOTW_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP9845185.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(16274..16432)
                     /locus_tag="ACHOTW_003128"
     CDS             complement(16274..16432)
                     /locus_tag="ACHOTW_003128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845186.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            16503..16904
                     /gene="pliG"
                     /locus_tag="ACHOTW_003129"
     CDS             16503..16904
                     /gene="pliG"
                     /locus_tag="ACHOTW_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP9845187.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(17007..17375)
                     /gene="ycgJ"
                     /locus_tag="ACHOTW_003130"
     CDS             complement(17007..17375)
                     /gene="ycgJ"
                     /locus_tag="ACHOTW_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP9845188.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            17895..18590
                     /gene="minC"
                     /locus_tag="ACHOTW_003131"
     CDS             17895..18590
                     /gene="minC"
                     /locus_tag="ACHOTW_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP9845189.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            18614..19426
                     /gene="minD"
                     /locus_tag="ACHOTW_003132"
     CDS             18614..19426
                     /gene="minD"
                     /locus_tag="ACHOTW_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP9845190.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            19430..19696
                     /gene="minE"
                     /locus_tag="ACHOTW_003133"
     CDS             19430..19696
                     /gene="minE"
                     /locus_tag="ACHOTW_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP9845191.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(20068..>20816)
                     /locus_tag="ACHOTW_003134"
     CDS             complement(20068..>20816)
                     /locus_tag="ACHOTW_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9845192.1"
                     /translation="DAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFD
                     RGGHGSVDSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNSDGNFVNDLSGRRGIYQAGIKASFSS
                     TLSGHLGVGYSNGAGMESPWNAVAGVNWSF"
BASE COUNT         5182 a         5303 c         5005 g         5326 t
ORIGIN      
        1 tggcaagaat gaaaccatca ccactccccg tctgagcttc cgttttctta acgtcagccc
       61 gacggtggag cggcaattac agcggattat tttctctctc gagcgggaag cccgggagaa
      121 agcggacaaa gtacgcgact gatttacatc gcgtccagtg cctgctcaag tttgtagata
      181 tagcgcggag cctgcggcgc aggatgattt cgcgctacgt gatcgaggaa ctcgtcagca
      241 tccagatcgt tgattttgct gattgccttt ttccgatctg acgagaaagt ccgtagcagc
      301 gcacctgccc cgttagcata tgacaccacc agcgcatatt gcagtacctt cggatcttca
      361 atgcctgcca gcgggccggt ttccagaata ttcaggtaag ccgcccccat agaaatatta
      421 cgctccggat ttttcagctc gctggtcgtc ggctcaccac tccagcccat acgccgataa
      481 acatcacgtc cggaggttga agcttttaac tgcatcaaac caatggcatt cgatttactc
      541 accgcgttcg gattaccacc cgattcgata gcgataatcg ccgtgatcaa ttgtggatcg
      601 acgccccagg ctgcaccggc tttctggctt attggcatcc actgcatcgc acgttgcacc
      661 ggaactttcg cgttccacgg cgggttcgta tagtcatgct ttgatgaaca acccgctaat
      721 aacacaatca aaaaggcaaa ccatctcaat ttcacttctt ctatccttat agcctggaat
      781 cgccgttgag gtgacaacgg cacacctaag cggcatgata tacatttcgt atctgttgtc
      841 agcaaggaat tgccatgtct ctgtttcact taattgcccc atcgggttac tgcattaaac
      901 agcacgccgc gctgcgtggt atccagcgcc ttaccgacgc ggggcatcaa gtcaataacg
      961 tcgaggttat tgcccgtcgt tgtgagcgtt ttgcgggtac ggaaacggag cgtctggaag
     1021 atctcaattc ccttgccaga ctgactaccc ccaacaccat cgtgctggct gtacgcggcg
     1081 gttacggtgc cagtcgttta ctggcagata ttgactggca ggcactggtg gctcgccaac
     1141 aatatgaccc gttgctcata tgcgggcata gcgattttac cgccattcag tgcggtcttc
     1201 tggcgcaagg caatgtcatc acctttagcg gccccatgct ggtagcaaac tttggcgcgg
     1261 atgaactgaa cacctttacc gagcaccatt tctggctggc gttacgcaat gaaaccttta
     1321 ccattgaatg gcaaggggaa ggcccgacat gtcgggccga aggcacgttg tggggaggca
     1381 atcttgcgat gctgatttca ctgatcggta caccgtggat gccaaaaatt gagaacggta
     1441 ttctggtgct ggaagatatt aacgagcacc ctttccgcgt cgaacgtatg ctgttgcagc
     1501 tctatcatgc gggaattttg ccacgtcaga aggcgattat tctcggtagc tttagcggca
     1561 gtacgcctaa tgattatgac gcaggttaca acctggagtc agtgtacgca tttttgcgtt
     1621 cccgcctgtc gattccactc attaccggcc tcgattttgg tcatgaacaa cgcacggtta
     1681 cgctgccgtt gggcgcacat gccattctta ctaacacccg ggaaggcact cagttgacga
     1741 ttagcggtca tcctgttctt aaaatgtaaa aaaaatcacg cttcagggct aagtaaaacg
     1801 ctgtctctgc cgctaatatg gtagggttta tataagagac agcgtaatca ggagtaaccg
     1861 accgttggat gccacaacaa taattagcct tttcatctta ggatccatcc ttgttaccag
     1921 cagtatatta cttagttcat tttcttctcg tcttggcatt cctattctgg ttatttttct
     1981 ggcgatcggc atgctggcgg gagtcgatgg cgtcggcggt atcccatttg ataattaccc
     2041 cttcgcctac atggtaagta acctggcgct ggcgattatt ttgctcgatg gcgggatgcg
     2101 cactcaggcc agttcctttc gtgtggcgtt aggaccggcg ctgtcgctgg cgacgctggg
     2161 cgtacttatc acctctggtt taaccggcat gatggcggcg tggctgttta atcttgattt
     2221 gattgaaggc ttattaatcg gcgctatcgt cggctccacc gatgctgcag cggtcttttc
     2281 tctgctgggt ggtaaggggc ttaacgaacg tgttggctcg acgctggaaa ttgaatccgg
     2341 cagtaatgat ccaatggcgg tctttctgac gattaccctg attgcgatga tccagcagca
     2401 tgaaagcagt gtcagttgga tgttcgtggt cgatattctg cagcaatttg gcctcgggat
     2461 tgtcatcggt cttggcggtg gttatttact gctgcaaatg attaatcgca tcgccctgcc
     2521 cgccggttta tatccattgc tggcattaag cggcggtatt ttaatttttg ctttaaccac
     2581 ggcgctggaa ggtagcggta ttctggctgt ttatctgtgc ggttttctgc tgggtaatcg
     2641 gccgattcgc aaccgctacg gcatcctgca aaatttcgac ggcctcgcct ggctggcgca
     2701 aatcgccatg ttcctggtgc tggggctgtt ggttaaccca agcgatctgc tgcctattgc
     2761 cattccggcg ctcattttgt ccgcatggat gattttcttc gcccgtcctc tttcggtatt
     2821 tgccggattg ctaccattcc gtggcttcaa tctgcgtgag cgcgtgttta tcagctgggt
     2881 gggattacgc ggcgcggtgc cgattattct ggccgtgttc ccgatgatgg cggggctgga
     2941 gaatgcacgt ctgttcttta atgtcgcctt ctttgtggtt ctggtttcac tgctcttgca
     3001 gggaacatca ctctcgtggg cggcgaaaaa agccaaagtg gtggttccgc ccgtcggacg
     3061 tccggtgtca cgcgttggcc tggatattca tccggaaaat ccgtgggagc agtttgttta
     3121 tcaattgagt gccgataaat ggtgcgtggg cgcggcactg cgcgatttgc atatgccaaa
     3181 agagacgcgt attgcggcac tgtttcgtga taaccagttg cttcatccta ccggcagcac
     3241 ccgactgcgc gaaggcgatg tgttgtgtgt aattggtcgg gaacgcgatc tcccggcgct
     3301 cggtaaactg ttcagccagt cgccgccggt cgcgctggat caacgcttct ttggtgactt
     3361 cattctcgaa gccagcgcca aatatgcaga tgtggcgctg atatatggcc tggaagacgg
     3421 gcgagaatat cgtgataagc aacaaacgct gggtgaaatt gtccagcagt tgttaggcgc
     3481 ggcaccggtt gtcggtgacc aggtagagtt tgccgggatg atctggacgg tggccgagaa
     3541 agaagataat gaagtgttga agattggcgt tcgagtggct gaagaggaag ctgaatctta
     3601 aatttaatga agcatctatc gccggttacg atgctttgct taacgcagcc tgagcctgta
     3661 tataaacttg tcatttcctg attttgatat gcacattcca acatcagaag aagcagcctg
     3721 ttaatcaaca ttctgaacat gaacccgaat ctgcatcaag ggtgaagcgt gatttatctg
     3781 aagtcgagtt cgagtcaaca atgaagcgta agcgttttat gtagaacgcc tgtgcggtga
     3841 gttcaggttc cggggaaata gtgtccatcc ttttaatggg cactatcatg ggcaatggct
     3901 ctgtcacatt aacaactaca gttgctgacc agccggatgg atgatttttt ttgcaaccag
     3961 aagacgttgc ctccgatccg acttacaaca agttacaccg tcacaaccgg gacgcgtaac
     4021 gccagcgcgc acatcaactc atagcccacc gttccggcag cggcggcgac atcatcaatt
     4081 ttgatctcct tgccccacag ctcaactggc gtaccaatac ccgcctgcgg gcaaggcgtt
     4141 aaatcgaccg ccagcatatc catcgagact gtccccaccg tcatggtgcg cacgccgtcc
     4201 actaaaacag gggtaccggt aggcgcgtgg cgcggataac cgtcggcgta tcctgcggca
     4261 acaatgccga ttcgctgctc atcgcgcgca gtataacgac cgccgtagcc cacacgctcg
     4321 cccgctttta gcgtctggac accaataatc tcactgctta gcgtcatcac cggacgtaat
     4381 ccagtattgg cgatatcacg ccactgaccg gacggcgaag cgccatacaa aataatgcca
     4441 ggccgaaccc agtcaaaatg cgcttctggg tgccacagag tcgccgccga attggacaac
     4501 gaacgccgac actccagccc ttccgccgcc tgctcaatac gcgccatcgc gccggaaatt
     4561 ccatcaggat gttccgcctc ggcaaaatgc gacatcaggg tcatttcgcc aacattcgcc
     4621 atcgcccgca actgctgcca gacggtaagc acgcgatcgg gctggaagcc caaccgattc
     4681 atcccactgt tcactttaag ataaatatcc aacggtgctt ttagccgcgc attttgcagt
     4741 gctttgagct gccagttgct gtgtacgcag gtggtcaggc ggtgctggtc atacatttcc
     4801 agatcctgag catggaaaaa tccttccagc atcaggatcg gtcctttcca gccgcgctca
     4861 cgtaacgtta ttgcttcttc caggttaagt aatgcaaagc catcggtagc cccgagcgcg
     4921 ctccagatac gctcaatacc gtgcccgtaa gcgttcgctt ttaccaccga ccagacgcgc
     4981 gcgtgcggcg cggcctggcg gacaatggac agattctgtt ttaatgcctg cagatcgagg
     5041 ctggcctgta tcggacgggt catctcgttt ccttagctgt gtgcgccatg taaatggccc
     5101 ggacgtgatg gcgtaaatcc acggctgtag cgcgctacgc ttagatcctc atatgggatc
     5161 gctggcgtgc gaccagagag cagatcgctt aacaactgac cggaaccgca agccatcgtc
     5221 cagccgagcg tgccgtgacc ggtatttagc cacagatttt taaagcgtgt acgcccgaca
     5281 accggcgtgc cgtctggcgt catcgggcgc agacccgtcc agaaagtcgc ctgctcgaca
     5341 tgaccgccgc gtggatagag atcgcgaacc accatctcca gcgtttcacg acgcggttgc
     5401 aacagctcgg tattaaaacc aacaatttcc gccattccgc caacacgaat gcggttatcg
     5461 aaacgggtaa tggcgatttt gtaggtttca tcaagaatgg tggataccgg cgcaccatct
     5521 tcctgcgcaa ttggaatggt cagcgagtag cctttcagcg gatacaccgg aatatcaaca
     5581 atgcctttga gcatcgccgt cgagtaagaa ccaaacgcca tcacatacgc atcggcctta
     5641 atcacttcat cgccacactt cacgccgtag atttgctcgc cgtcgcaaag cagttggtca
     5701 acgggcgtat taaagcggaa tttaaccccc gcctgctccg ccatccgcgc cagattctgg
     5761 gtaaacagct ggcagtctcc ggtttcatca ttgggcaatt gcagaccacc cgtcagtttg
     5821 tgcgctactt ctgccagcgc gggctccact tccgccaggc ggctggattc cagcagctga
     5881 tacggtacgc cggcatcttc cagcacggcg atatcgcggg tcgcattttc atactgttgt
     5941 tcggtacgga acagttgcag cgtcccaccc tgacgccctt catactgaat attggtttcg
     6001 gcgcgcaatg ctttcaggca atcacggctg tattccgcca gacgcaccat ccgccctttg
     6061 ttttccatgt agtggctggt gtcgcagtta cgtaacattt gccacatcca tttcaactgg
     6121 aactgcgtac cgtcgagacg aaccgccagc ggcgcatggc gctggaacat ccatttaatc
     6181 gcttttaaag gcacacctgg tgccgcccac ggtgcagcat atccggggga gatttgcccg
     6241 gcattcgcgg cactggtttc cagggcggcc cccggctccc gatcaatgac ggtgacctca
     6301 tgtcctgcct gatttaagta ccaggcgctg gcaacgccta ccacaccact tcccagtatg
     6361 acaactcgca tagccactcc gataacggta gatacagtaa aagaataata atctaattac
     6421 atcttgataa ctcagttgaa aatattattc aacatacggc ttttttatgg tgagctggct
     6481 cacatctccc tgactctgcg gcaatatggc gtctttagta tctcctgctg aacgcccttt
     6541 ttatccagca taaaattccg ttcagaaacg gattagtggc actctgataa taagaaatcg
     6601 caaagaaaaa aattctccgg cgacgcggtt tttaacaggt gttctatgct tgaaatgagg
     6661 tgctccacac agagagcgcc gacaacaatg aggtgctcca cacagagagc gccgacaaca
     6721 atgaggtgcg cgtatggcga cgatcgattc tatgaataag gacaccacac gtttgagcga
     6781 tggacccgac tggacgttcg acctgctgga tgtttatctg gcagagatag accgggtggc
     6841 gaaactctac cggctggata cctacccgca ccagattgaa gtgataacct cagaacagat
     6901 gatggatgcc tactccagcg tcggcatgcc aattaactat ccgcactggt cattcggtaa
     6961 aaagtttatc gagactgaac ggctgtataa gcacggtcag caaggactgg cctatgaaat
     7021 cgtcattaac tctaacccgt gtatcgctta cctgatggaa gagaacacca ttaccatgca
     7081 agcgctggtg atggctcatg cctgctatgg gcataactct ttcttcaaaa acaattactt
     7141 attccgtagc tggaccgacg ccagttcgat tgtcgattat ctgatttttg cccgtaaata
     7201 tattaccgag tgcgaagaac gttatggcgt tgatgaagta gaacggcttc tggactcgtg
     7261 ccacgcgctg atgaactacg gcgtggaccg gtacaaacgc ccgcaaaaaa tctcgctgca
     7321 agaagagaaa gcccggcaga aaagtcgcga agagtatttg caaagtcagg tcaatatgct
     7381 ctggcgtacc ctgccgaagc gcgaggaaga gaaaacggtt gctgaagcgc gccgttatcc
     7441 gtccgaacca caagaaaacc tgctctattt tatggagaaa aatgcgccac tgctggaatc
     7501 atggcagcgt gaaatcctgc gtattgtgcg taaggtgagc cagtattttt atccgcaaaa
     7561 acagactcag gtgatgaacg aaggctgggc caccttctgg cactacacca tccttaacca
     7621 tctgtatgat gaagggaaag taacggaacg ttttatgctg gagtttttgc acagccacac
     7681 caatgtggtc ttccagcccc cctataacag cccgtggtac agcggcatca acccgtatgc
     7741 cctcgggttc gccatgttcc aggatattaa acggatttgt cagtcgccaa cggaagaaga
     7801 caaatactgg ttcccggata tcgccggttc cgactggctg gaaacgctgc atttcgcgat
     7861 gcgtgatttc aaagatgaga gttttatcag ccagttcctg tcgccgaaag tgatgcgtga
     7921 tttccgcttc ttcaccgtgc tggatgatga tcggcataat tatctggaga tttccgctat
     7981 tcataatgaa gaaggttatc gggagatccg taaccggcta tcgtcgcaat ataacttaag
     8041 taatctggag ccgaatattc agatctggaa cgtggatttg cgcggcgacc gttcgctgac
     8101 gctgcgttac attccacata atcgcgcacc gctggatcgg gggcgcaaag aagtactgaa
     8161 gcatgtgcat cgcctgtggg gatttgatgt gatgctggaa cagcaaaacg aagacggcag
     8221 cgtcgagttg ctggaacgtt gcccgccgag aatgggaaat ctgtaaaaac aacaaaaaac
     8281 ccctcgtttg aggggtttgt tttttaaacg gaagggatta tcgcccctga atggctaaat
     8341 cacccggcag atttttctgc atccggtgcc aaatctcgcc actctcatgc ccatagcgac
     8401 gcactgtttc gtacacctga tcgtgcgcgc cttcactgca caacgccgac agtttgtggt
     8461 agaagcccag cgccagactg cgcgcttccg gattggcgaa atagtgacga ccaatgcgcg
     8521 tatacagtcc cttcatcccg ttaaggatca gaccgtaaat cgggttgccg gaagcaaacg
     8581 ccaggccgcg gaatatgttg taatccagct cggcaaaggc atcggcgtga tcggccactt
     8641 cattagcggt agccagcact tcctgcgctt tatcgggatg ctgacgaaac gcggtgcgaa
     8701 taaaaatagt tgaaatattg gtacgcaccg acagcaaatt atcaataagc tgcggcacac
     8761 tttcgtgatc cagtcgcgcc agtgtttcaa ggatatttaa accagaggtt tcccagaaat
     8821 tattcacctt cgtcggcttg ccatgttgaa tggtcaacca gccatctcgt gccagacgct
     8881 gtaacacttc acgtaacgta gtacgcgtta cgccaattaa ttctgaaagt tcacgttctg
     8941 cgggcaaaat agtcccggga gggaagcggt tattccagat actttcaata atgtactctt
     9001 ccgcgaaacc cgccgggctt tgcgccttaa tgaccatagt gagatttcca taacacagca
     9061 aaacaaagtt ggactcatca taccagaggg ctaactacgc tgatagcaga caaaaagaag
     9121 aaaaaaggga tcaaggcttc attttccgca tctctttccc cttgactatc aagacttgcc
     9181 tcactcctgc ctgaccgtta agctttgcgt ttaaacataa aaacatcatt gattatttgt
     9241 aaggtaaggg aaaccatcat ggagatctcc tggggccgcg cgctatggcg caattttttg
     9301 ggccagtccc ccgactggta caaactcgcc ctcatcattt tcttaatcgt aaatccgtta
     9361 attttcctca tcagcccttt cgtcgctggc tggttgctgg tagcggaatt tattttcact
     9421 ctggcgatgg ccctgaaatg ctacccgctg ctccccggtg gcctgttggc tatcgaagcg
     9481 gtattcatcg gcatgaccag cgcggaacac gtccgtgaag aggtggcggc aaatcttgaa
     9541 gtcttgctgt tactgatgtt tatggtggcg ggtatctatt ttatgaaaca gctattgctg
     9601 ttcatattta cccgtttgct gttaagcatt cgctccaaaa tgctgctgtc gctctctttt
     9661 tgcgtagcgg cagcgttcct ctccgcgttc ctcgatgcct taaccgtcgt ggcggtggtg
     9721 atcagcgttg ccgtcggttt ttatggtatt tatcatcgcg tagcctcttc ccgtaccgaa
     9781 gacaccgacc tgcaagacga tagtcatatc gacaagcatt acaaagtggt tctggaacag
     9841 ttccgtggct ttctgcgtag cctgatgatg catgccggtg tcggcaccgc attaggcggc
     9901 gtaatgacca tggtgggcga accacagaac ctgatcatcg ctaaagcggc tggctggcat
     9961 tttggcgatt tcttcctgcg catgtcgccg gtgaccgttc cggttctgat ttgtggcctg
    10021 ttaacctgcc tgctggtaga gaagctgcgt tggtttggct acggtgaaac gctgccggag
    10081 aaagtccgcg aagtgttgca acagtttgac gatcaaagcc gccaccagcg tacccgtcag
    10141 gataaaatcc gtctgattgt ccaggcgatt attggcgtct ggctggtgac tgcgctggcg
    10201 ttgcatctgg cggaagttgg cttgattggt ttgtcagtca ttattctggc aacatcattg
    10261 accggtgtca ccgatgaaca tgctatcggt aaagccttca ccgaatctct gccattcacc
    10321 gcactgttga cggtgttttt ctcggtagtc gcggtgatta tcgaccaaca actgttttcg
    10381 ccgattattc agtttgtgtt gcaggcatcg gaacatgctc agctgtcgct gttctatatt
    10441 ttcaacggtc tgctgtcatc catttcggat aacgtcttcg tggggacgat ttatatcaac
    10501 gaagcgaaag cggcaatgga aagtggcgct atcacgttga agcaatacga gctgctggcg
    10561 gtcgccatta ataccggtac caatctgccc tctgtcgcta cgccgaacgg tcaggctgcg
    10621 ttcctgttcc tgctgacctc tgcactcgcg ccattgattc gcctctctta tggccgcatg
    10681 gtgtggatgg ccctgcctta caccctcgtc ctgacactcg tcggactgct ctgcgtcgag
    10741 tttacgcttg cccctgtaac cgaatggttt atgcaaatgg gctggatagc aacgctttga
    10801 taacaactta ccgggcatat aaatgcccgg tttgcctttt cgcccgataa ttgtccaatt
    10861 gcgtcctatt tttgctgcct cctggtggcg cagcgaatga attggtttaa actgcgcact
    10921 ctatgcatat tgcagggaaa tgattatgtt acgatttttg aaccaatgtt cacaaggccg
    10981 gggcgcgtgg ctgttgatgg cgtttactgc tctggcactg gaactgacgg cgctgtggtt
    11041 ccagcatgtg atgttactga aaccttgcgt gctctgtatt tatgaacgct gcgcattatt
    11101 cggcgttctg ggtgctgcgc tgattggcgc gatcgcccca aaaacgccac tgcgttatgt
    11161 agcgatggtt atctggttgt atagtgcgtt ccgcggtgtg cagttaactt acgagcacac
    11221 catgcttcag ctctatcctt cgccgtttgc cacctgtgat tttatggttc gtttcccgga
    11281 atggctgccg ctggataaat gggtgccgca agtgtttgtc gcctctggcg attgcgccga
    11341 gcgtcagtgg gattttttag gtctggaaat gccgcagtgg ctgctcggta tttttatcgc
    11401 ttacctgatt gtcgcggtgc tggtggtgat ttcccagccg tttaaagcga aaaaacgtga
    11461 cctgttcggt cgctaatcca ttcggcgctc ctgcgggagc gcttttttcc tgccgctgtg
    11521 tttatttgac ccgcagtaaa tcagaacttc gcgttgtata acgtggtgaa agcatggctc
    11581 gcttcatctg ccattgttgc tggatcccct gcccggcaaa atagagtgtt cctctgccct
    11641 ctttagcatt cagtgtatcc attaccgcca tcaattgctc actcccgggg cgcggagcgt
    11701 tgtcatcgaa taaattgagc tgcgcgactc cctgactgaa gaaatccccc agcatcacgc
    11761 ccgctttttg gtagcgatgg cccgcttgcc agatggcatc cagagatcgc gtagcggcgt
    11821 taatgatatc cctgctgtcc tgagtgggcg tcagcagttt taccgacgcg ctattgccgt
    11881 aataaggttc attgagcgca aatggtgacg tcttaataaa tgtggagata aaccgacaat
    11941 attgatgctc actgcgaagt ttttccgccg cccgggcagc gtaactacaa atggcctgcc
    12001 gcatcgacgt ataatccgtg atgcgttcac caaacgagcg ggaacagata atttcctgct
    12061 ttgtcggtgc aaattcttcc agttgcaaac agggttcacc gcgcagttca cgcaccgttc
    12121 tttcgagcac gacattaaaa tgtttacgga taaaccggat atctgtatcc gccaaatcga
    12181 gaacggtttt gatccccatc gcgtccagtt ttttgctgat ccgccgtcca atcccccaga
    12241 cgtcatccac ggggagagca gacattaatt tacgctggcg ttccagattt gataaatcca
    12301 ccaccccacc cgtctgccgc tgccattttt ttgccgcatg attggcaagc ttagccagcg
    12361 ttttggtctg ggcgatcccc acaccaacag taagatgggt acgttgtagc accgttgcgc
    12421 gaatttctct gccaaagtca gtcagatcgc gacaattacg cacacctgtc agatcgcaga
    12481 atgcctcatc aatactgtaa atctcgacgc ggggcgatag ttcttccagc gtcgacatca
    12541 cccgattgct catgtctgcg taaagctcat aattgctgct aaagcaaacc acgccacagc
    12601 gacgaaacag atctttttgt ttgaaccagg gatcgcccat tttaacgcca agcgcctttg
    12661 cctcagcgtt tcgggcgata acgcaaccgt cattattcga tagcacaacc accggtttac
    12721 cccataactc agggcgaaac accgtctcac agctggcata aaacgcgttt acatcacaga
    12781 gggcaaacat cagcgcatcg ccttaacgac gtggatcacc acaccaaaga catccagcgt
    12841 atcttcacta ctgatggtaa tgggcgagta cgcgctgttc atgggaataa gctgtaccgt
    12901 cgggcgtagt tgcaattttt tcaccgtaaa ctcgccgtca acagcagcga tgacaatatc
    12961 accatggctg gcggtaatag cgctatcgac aatcagtaaa tcaccgtcac taattccacc
    13021 atcaatcata gaatcaccac ttgctttgac gaagtaagtc gcgctgggat gctggatcaa
    13081 cagttgattc agatcgatgc gctgttcaac gtaatctgcg gccggtgaag gaaagccaca
    13141 ctgaacaaga tcgctaaata gcggaaaagt cacaatttcg cggagatccg caggcttgat
    13201 aaacaacata ataatctgcc tgaagttata ctgtttttat atacagtagt ctgttcttgc
    13261 cagcagatca atactgattc aggctatcaa tatttgtcgc tgcatagact gctgattttt
    13321 cgttctctta tcctgtgctc acgcggcctt ctggcgacga cgctcatcca gcagaaatga
    13381 aaaatatcac ccggctaaaa aatagaatag aagcattcgc cataatgaca ttaaacattg
    13441 tttgatattt atcatattaa tagaaataaa gacattgacg catctctccc ggctaactat
    13501 gaattagatg aagtaaaatt cattaatagt tgtaaaacag gagtttcatt acaatttata
    13561 tatttaaaga ggcgaatgat tatgactgaa atcgttgcag ataaaacggt agaggtagtt
    13621 aaaaacgcaa tcgaaaccgc agatggagca ttggatcttt ataataaata tctcgatcag
    13681 gtcatcccct ggcagacctt tgatgaaacc ataaaagagt taagtcgctt taaacaggag
    13741 tattcacagg cagcctccgt tttagttggc aatattaaaa ccttactaat ggatagccag
    13801 gataagtatt ttgaagcaac ccaaacagtg tatgaatggt gtggtgttgc gacgcaattg
    13861 ctcgcagcgt atattttgct atttgatgag tataatgaga agaaagcatc cgcccagaaa
    13921 gacattctca ttaaggtact ggatgacggc atcacgaagc tgaatgaagc gcaaaaatcc
    13981 ctgctggtaa gctcacaaag tttcaacaac gcttccggga aactgctggc gttagatagc
    14041 cagttaacca atgatttttc agaaaaaagc agctatttcc agtcacaggt agataaaatc
    14101 aggaaggaag cgtatgccgg tgccgcagcc ggtgtcgtcg ccggtccatt tggtttaatc
    14161 atttcctatt ctattgctgc gggcgtagtt gaagggaaac tgattccaga attgaagaac
    14221 aagttaaaat ctgtgcagag tttctttacc accctgtcta acacggttaa acaagcgaat
    14281 aaagatatcg atgccgccaa attgaaatta accaccgaaa tagccgccat cggggagata
    14341 aaaacggaaa ctgaaacaac cagattctat gttgattatg atgatttaat gctttctttg
    14401 ctaaaagaag cggccaataa aatgattaac acctgtaatg agtatcagaa aagacacggt
    14461 aagaagacac tctttgaggt acctgaagtc tgatcagcaa ttattctctc catgtactca
    14521 aggtataaag tttatcacat ttaaaaatac agacgttttt taaagattga gagttacctg
    14581 ttgataataa ggtatcgggt aactctcatt atgacagagc gctttcaaaa acagggctac
    14641 gaaatggcga gcatccagac gtatattaag aaagccccca tcaacatcta ttaaatttat
    14701 cactgcgccc agtcaggttt atttaagata tgatcctgcc agtcaatcac ttctgattct
    14761 ttcactgcga tatgacgcac agagatacgt tcaccatgca ttgccgcttt cgaaccagta
    14821 agcagcggat gccacgcagg taaatcttta ccttccgcca gcaaacgata agcgcaggtc
    14881 attggtagcc attcgaatgt tggcagattt tcacgggtta atttaatgca gtcgggttca
    14941 aactcgaaac gacgttcgta gttccgacat tgacaggttt taatattgag ctggcgacag
    15001 gcgacgttag tgaagtagat ttcgtcggtg tcttcatcca tcagtttatg caggcaacac
    15061 tgaccgcaac catcacacaa cgactcccat tccgcatcgc tcatttcgtc cagggtttta
    15121 ctttgccaga aaggtacatc gctcatcagg tgttccgcca ttacgttaaa acgcacctta
    15181 taaccagtct ggcacagcga tgcaagtttt gccgccgctt tcaggcggca aaaagtatta
    15241 caaaacgcga gttgtcaaag aatgcccatc gaaagtgact gtcagctcat caccgctttg
    15301 caacgggccg acgccatcag gcgtgcctgt cagcacaacg tcaccggcct tgagggtaaa
    15361 aaacttgctc atataagcga tcagcggaac gattttatgg atcatgtccg cagtcgtacc
    15421 ttgctggcgt tgttcgccgt ttacgctcag gcccagcgtt gtattttgcg gatcgccggt
    15481 gaattccgcc gcgggaataa acccggaaag cggacaagag ttatcaaacg ctttagcctt
    15541 ttcccacggc tgcccggctt tcttcatttt tccctgaaca tcacgcaacg tcagatcgag
    15601 cgccacgccg taaccggcaa tggctttgcg gacatgctct tccgtagcct gacgcagcgt
    15661 cgcgccaatc aacaccgcta gttcgacttc atgatgaact gaaccgaaat cggatgggat
    15721 cgccaatggc tgccgcagat cgcacagtgc cgtttctggt ttaataaaca gcactggctc
    15781 ttcgggcact gcgctgccca tctctttaat atgtttggca tagttactgc caacacaaac
    15841 tactttactc accggataat ccagcagcgc accttgccag ttgtgatgtt gatacataac
    15901 gttcccctca ccttatcgtg gatatgaaaa cccgcgccga caaagataat gccaccgccg
    15961 gatatcggtt atttgttagt gtcgtctgtt ttctgcccca tgacggaaag atgttgcttc
    16021 agcaaatctt cgggtggtgg cggtaactgc aaatagtaac cttgctcggt taatgcctgt
    16081 ttaacctttt caatatcggc attgaccagt ttcttccgcc catccagcgg cagaatcatc
    16141 gctaactgag gctgaccaaa acctttcatc agttcctcag gaacacgcga aaaatcgtct
    16201 tttttttcga catataaata ggtctggtca cgcttgctgc ttcgataaat cacacaaaac
    16261 atacttttac tctttaagat gccaggtttt ggcataagct aacatcaatg aaacccgcgc
    16321 cacaagccag ccagctgtaa atttctacgg tttgttgagt ccatgcccct ttataagtaa
    16381 tccccccggc aaaaatgccg cgacgggatt ttaccgaacg caggaaagcc actggaattc
    16441 tcccgtaagg gattgttata ttataacagt tcatcgtact cattctgaac agaagactac
    16501 caatgaaaat caagagcatc agtaaggctg tattgctgct tgcactgcta acctccacga
    16561 gctttgctgc gggtaagaat gtcaatgttg agttcagaaa aggccatagc agcgctcagt
    16621 actccggcga aataaaggga tacgattacg atacatatac cttctatgcc aaaaaaggtc
    16681 agaaagtgca tgtgagtatc tctaatgaag gcgctgatac ttacctcttc gggccaggaa
    16741 tcgatgattc tgttgattta tccagatatt cccctgaact cgatagtcac ggacaatact
    16801 cactccctgc ttctggcaaa tatgagctgc gagtgctgca aacccgaaac gacgcccgta
    16861 aaaacaaaac taaaaaatat aacgtcgata ttcaaattaa gtaaatgtca accgacgggg
    16921 agtctccccg tcataaacgt tgacgcccca ctcctctctg tcgctcaaga ggttaatgac
    16981 gaatagaaca taatgtaaac aagacatcac tcacgacaca taaataacat ctttgtggtg
    17041 gtttgattga tttttccact ccgcttacca tctacaccaa aataacgatc atcacggcat
    17101 aatttttctt taacatcgca aaatacaccg ttagcgaatg taaattcgct gggatcgtag
    17161 ccttgtaatg attgtaagtt ttcagcggct ttttcgctga gatatttcct tgttaactct
    17221 ggggaaatgc catctgagtt cacacaaata tacgggtcgc aaacaacgcc gcacgccgga
    17281 gattgaagtc cgccagcgtc atgtccagac gccattgatg agaacatcat ccccacacct
    17341 gacaatccga tataaaaaat acgcatcata ttcatagcac atcctgataa tccaaccgta
    17401 caacacttat gctcatttca tcatcaatcc gtaatcaaaa ccgctgacct ggacgagtta
    17461 cgatgacctg acagaagtat cagacgtctt ctgcggtttc tatgatagag caattctgtg
    17521 gcggatatta agataacggg tatctgttct ttaatgatgc tgcaaaaaat ggaaagttaa
    17581 gaaagcctgg cgtggtgaaa aaatacactt gcctcaatac tggatattta aacaggtctg
    17641 gagatgctgt cacgattagg gtgcggaaaa aggaagcgta aataagaaga atcaaaagta
    17701 cgacggcaat gggttgattg acaagggtat tttttaagct atgaatcagc gccatttatc
    17761 acagaataga cttttactct gaataaatgg gagggtgact tgcctcaata taatccagac
    17821 tataacatgc cttatagtct tcggaacatc atcgcgcgct ggcgatgatt aatagctaat
    17881 tgagtaaggc caggatgtca aacacgccaa tcgagcttaa aggcagtagc ttcactttat
    17941 ctgtggttca tctgcatgag gcagaaccta aggttatcca tcaggcgctg gaagacaaaa
    18001 tcgctcaggc ccccgcattt ttaaaacatg cccccgttgt actcaacgtc agtgcactgg
    18061 aagacccggt aaactggtca gcgatgcata aggcggtttc ggcaaccggt ttgcgggtta
    18121 ttggcgtaag tggctgcaaa gatgcgcaac ttaaagccga aattgaaaaa atggggctgc
    18181 ctatcctgac ggaaggaaag gaaaaagcgc cacgtccagc tcccgcaccg caggctccag
    18241 cgcaaaatac aacgccggtc acaaaaacgc gtttaataga taccccggtg cgttccggtc
    18301 agcgtattta tgctccacaa tgtgatctga ttgttacaag ccacgttagc gctggggccg
    18361 aattgattgc cgatgggaac attcatgtct atggcatgat gcgcggtcgt gcgctggcag
    18421 gggcaagtgg tgaccgggaa acgcaaatat tttgtacgaa cctgatggcg gaattggtgt
    18481 ccatcgcagg tgaatactgg ctgagtgatc aaatcccagc agaattttat ggcaaagcgg
    18541 cgcgactgca gttagtcgaa aacgctttga ccgttcaacc gttaaattga tcccttttta
    18601 acaaggaatt tctatggcac gcattattgt tgttacttcg ggcaaagggg gtgttggtaa
    18661 gacaacctcc agcgcggcca tcgccactgg tttggcccag aagggaaaga aaactgtcgt
    18721 gatagatttt gatatcggcc tgcgtaatct cgacctgatt atgggttgtg aacgccgggt
    18781 cgtttacgat ttcgtcaacg tcattcaggg cgatgcaacg ctaaatcagg cgttaattaa
    18841 agataagcgt actgaaaatc tctatattct gccggcatcg caaacacgcg ataaagatgc
    18901 cctcacccgt gaaggggtcg ctaaagttct tgatgatctg aaagcgatgg attttgaatt
    18961 tatcgtttgt gactccccgg cagggattga aaccggtgcg ttaatggcac tctattttgc
    19021 agacgaagcc attattacca ccaacccgga agtctcctca gtacgcgact ctgaccgtat
    19081 tttaggcatt ctggcgtcaa aatcacgccg cgcagaaaat ggcgaagagc ctattaaaga
    19141 gcacctgctg ttaacgcgct ataacccagg ccgcgtaagc agaggtgaca tgctgagcat
    19201 ggaagatgtg ctggagatcc tgcgcatcaa actcgtcggc gtgatcccag aggatcaatc
    19261 agtattgcgc gcctctaacc agggtgaacc ggtcattctc gacattaacg ccgatgcggg
    19321 taaagcctac gcagataccg tagaacgtct gttgggagaa gaacgtcctt tccgcttcat
    19381 tgaagaagag aagaaaggct tcctcaaacg cttgttcgga ggataagtta tggcattact
    19441 cgatttcttt ctctcgcgga agaaaaatac agccaacatt gcaaaagaac ggctgcagat
    19501 tattgttgct gaacgccgtc gcagcgatgc agaaccgcat tatctgccgc agttgcgtaa
    19561 agatattctt gaggtcattt gtaaatacgt acaaattgat cctgagatgg taaccgtaca
    19621 gcttgagcaa aaagatggcg atatttctat tcttgagctg aacgtgacct taccggaagc
    19681 agaagagctg aaataatccc gctgtaaaag cgcatttatc ttcaaggcag agttatctct
    19741 gccttgagtt tttcatccct ctcatccacg ttgtggtaaa gcggcgagta ttcttgctga
    19801 tactcctcat tgctatttcc acctccccct ttacacctta aggctgttcc cacgcacaaa
    19861 acaaaatatt tatgcctggt tgagtaaata accttattgt tagtgttgat atactgggag
    19921 atgataaccc cgcaatttca ttattcattg ttctcccata ggatgaggtt tcccgccgta
    19981 actggcgggc tttttttacc ccagaatttt ctcttttaac atcctgtaaa cgaaaactgc
    20041 gccgaagcgc agtttaatcg ttgatggtca gaacgaccag ttcacaccag ccaccgcgtt
    20101 ccacggggat tccataccag caccgttgct ataccccacc ccaagatgcc cgcttaacgt
    20161 actgctgaat gaggctttaa tacctgcctg gtatattcca cgtctgcccg acaaatcatt
    20221 gacgaaatta ccgtcactat tcactttcac ccggttatca tcgacaaatt ctttgcgcac
    20281 agccgccttc agccacggct caacttccat accgttcccc agacgcatgt tgtaactcag
    20341 cgttgcaccc agttcacgat atatactgcg ggtatcgact gatttcgatt ccatgccatt
    20401 ggataaatga tattcggggt tatcagcggt gaaccccgtt aacgaggcat acggcgtcag
    20461 gttccagtta ccatcggtaa atcgcatccc ggtttcaatg tgaccgccca gcccgttgct
    20521 atggtaactg ccattggcgg ctccaccgct gctcatttta ccggctacgt tactttcaaa
    20581 acggttcagc ttcacgacac cgtccagata gaaaccactt tcatgttccc aactggcata
    20641 gccgcccaga gaataactgt ccacactgcc atgtcctcca cgatcaaaac cgatatgtga
    20701 atgggaataa cccataaaag cgcccagcgt cgcaatcccc tcaggaatat cattacggct
    20761 gtcgatcccc actgtcattc cggtcagcgt ctgctcaaac ccggcacccg catcag
//
LOCUS       DAZKIF010000087        20740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZKIF010000087 DAZKIF010000000
VERSION     DAZKIF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.087"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..1650)
                     /gene="actP"
                     /locus_tag="ACHOTW_003135"
     CDS             complement(1..1650)
                     /gene="actP"
                     /locus_tag="ACHOTW_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP9845193.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1647..1961)
                     /gene="yjcH"
                     /locus_tag="ACHOTW_003136"
     CDS             complement(1647..1961)
                     /gene="yjcH"
                     /locus_tag="ACHOTW_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP9845194.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2162..4120)
                     /gene="acs"
                     /locus_tag="ACHOTW_003137"
     CDS             complement(2162..4120)
                     /gene="acs"
                     /locus_tag="ACHOTW_003137"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP9845195.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4513..5949
                     /gene="nrfA"
                     /locus_tag="ACHOTW_003138"
     CDS             4513..5949
                     /gene="nrfA"
                     /locus_tag="ACHOTW_003138"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP9845196.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5994..6560
                     /gene="nrfB"
                     /locus_tag="ACHOTW_003139"
     CDS             5994..6560
                     /gene="nrfB"
                     /locus_tag="ACHOTW_003139"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP9845197.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6557..7228
                     /gene="nrfC"
                     /locus_tag="ACHOTW_003140"
     CDS             6557..7228
                     /gene="nrfC"
                     /locus_tag="ACHOTW_003140"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP9845198.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7225..8181
                     /gene="nrfD"
                     /locus_tag="ACHOTW_003141"
     CDS             7225..8181
                     /gene="nrfD"
                     /locus_tag="ACHOTW_003141"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP9845199.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8201..9919
                     /gene="nrfE"
                     /locus_tag="ACHOTW_003142"
     CDS             8201..9919
                     /gene="nrfE"
                     /locus_tag="ACHOTW_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP9845200.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            9912..10295
                     /gene="nrfF"
                     /locus_tag="ACHOTW_003143"
     CDS             9912..10295
                     /gene="nrfF"
                     /locus_tag="ACHOTW_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP9845201.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10292..10888
                     /gene="nrfG"
                     /locus_tag="ACHOTW_003144"
     CDS             10292..10888
                     /gene="nrfG"
                     /locus_tag="ACHOTW_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP9845202.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11230..12543
                     /gene="gltP"
                     /locus_tag="ACHOTW_003145"
     CDS             11230..12543
                     /gene="gltP"
                     /locus_tag="ACHOTW_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP9845203.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12721..13410)
                     /gene="yjcO"
                     /locus_tag="ACHOTW_003146"
     CDS             complement(12721..13410)
                     /gene="yjcO"
                     /locus_tag="ACHOTW_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP9845204.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13504..15651)
                     /gene="fdhF"
                     /locus_tag="ACHOTW_003147"
     CDS             complement(13504..15651)
                     /gene="fdhF"
                     /locus_tag="ACHOTW_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15232..15234),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFP9845205.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15849..17315)
                     /gene="mdtP"
                     /locus_tag="ACHOTW_003148"
     CDS             complement(15849..17315)
                     /gene="mdtP"
                     /locus_tag="ACHOTW_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP9845206.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            complement(17312..19363)
                     /gene="mdtO"
                     /locus_tag="ACHOTW_003149"
     CDS             complement(17312..19363)
                     /gene="mdtO"
                     /locus_tag="ACHOTW_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP9845207.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19363..20394)
                     /gene="mdtN"
                     /locus_tag="ACHOTW_003150"
     CDS             complement(19363..20394)
                     /gene="mdtN"
                     /locus_tag="ACHOTW_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP9845208.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20413..20688)
                     /gene="ytcA"
                     /locus_tag="ACHOTW_003151"
     CDS             complement(20413..20688)
                     /gene="ytcA"
                     /locus_tag="ACHOTW_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP9845209.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4669 a         5496 c         5669 g         4906 t
ORIGIN      
        1 ttaatgcgcg cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg
       61 gaacagttca cgctcacgcg cgccttccgc tgagttatcg gttgccgaga agaaccagat
      121 gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga agatggcttt
      181 ttcgtgaccg aggatctgta cccaaatcgt cgggccgagg atcatcagta ccactgcggt
      241 aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg accagtacat
      301 agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca gccccaccat
      361 aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca cacccaaaat
      421 caggacggtg attttcgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac
      481 gttagcgtac aggtcatgcg aaaccgctga tgcgcccgcc agcgtcagac ccgcaaccac
      541 cgcgaggata gtggcgaaag caaccgctga aataaagccg aggaacaggt tgccgcccac
      601 cgcattggcc aggtgaactg ctgccatgtt gttaccacca atcagatggc ccgccgcgtc
      661 tttatattcc ggattcgcac cgaccagcat gatcgcgccg aagccgataa taaaggtcag
      721 gatatagaag tagcccataa atccggtagc gtagaacacg cttttacgcg cttcgcgggc
      781 atcgctgacc gtaaagaagc gcatcagaat gtgcggcaag ccagccgtac caaacatcag
      841 tcctagcccc agagagagtg cggagatcgg atctttcacc agtccgcccg gtttcatgat
      901 gtcgacacct ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc
      961 gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt
     1021 gataatctgt acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag
     1081 cacgccgacc agcaccactg caatgtgata gttaaggcca aacagcagct cgatcagttt
     1141 acctgcaccc accatctggg cgataaggta aagcgccacc accaccagag aaccacaggc
     1201 cgaaagaata cgaatcggcc cttgtttcag acgataagag gctacatcgg caaaggtgta
     1261 acgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc agcccaccag
     1321 gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagtg cggagatccc
     1381 caaaaatgag gcggcggaca tatagtcacc ggcaatcgcc agcccgttct gaaaaccggt
     1441 gatattgccg cctgcggtgt aatagtcatt acgcgaacgt acgcgttttg acgcccagta
     1501 ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt
     1561 tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt
     1621 ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta
     1681 ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccagtgag aacaaaggag
     1741 atcactatta ccccaacacc gactggaata ccgcgtgtga cgctggtgtt cggattcagc
     1801 ggggtgccca gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc
     1861 agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa
     1921 tgcgcattgt cttctatccg ctgataaata gtgccattca tcacagattc tccagaggta
     1981 atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc
     2041 ttgccgaatg taggccggat aaggcgttat acgccgcatc cggcaatcaa tgcctgatgc
     2101 gacgctgtcg cgtcttatca ggcctacaaa ccgttaccga ctcgcatccg gcaattgtgg
     2161 gttacgatgg catcgcgata gcctgcttct cttcaagcag cttctcgact acgccaggat
     2221 cggcaagcgt cgaggtatcg cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa
     2281 tacggcgcat aattttgccg gagcgggttt taggcaggga gtcggtccag tgcagcacgt
     2341 ctggcgtcgc cagcgggcca atctctttac gcacccagtt gcggacttct gcgtacagtt
     2401 ctggtgacgg ttcctccccg tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa
     2461 tattgtgcgg aataccgacg acagcggctt cggcgatttt cggatgcgcc accagcgccg
     2521 actcaatctc cgccgttccc agacggtgac cggagacgtt cagcacatcg tccacacgcc
     2581 cggtgatcca gtaatagcta tcttcatcac gacgcgcgcc gtcgccgctg aaatacatat
     2641 ttttgaaggt ggaaaaatag gtctgctcaa aacgttcgtg atcgccaaac agcgtacgcg
     2701 cctgacccgg ccaggagtcg gtgatcacca ggctaccttc ggtagcccct tccagcgggt
     2761 taccttcgtt atcgaccagc gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg
     2821 ctttcagctc ggtagcgcca ggcagcgggg tgatcatgaa accgccggtt tcggtctgcc
     2881 accaggtatc gaccaccgga catttctcgt tgccgatttt tttccagtac cactcccacg
     2941 cttccgggtt aattggctcg cccacggaac cgagaatgcg cagcgacgaa cggtcggtgc
     3001 cttcgatcgc tttatcgcct tccgccatca gcgcgcgaat cgccgtgggc gcggtataga
     3061 gaatattgac ctgatgcttg tccaccacct gtgccatacg ggcaggcgtc ggccagttcg
     3121 gtacgccttc aaacatcagc gtggtcgcgc cgcaggccag cgggccgtac agcaaataac
     3181 tgtgtccggt cacccagccc acatcggcgg tgcaccagta gatatcgccc ggatgataat
     3241 caaagacata tttaaaggtc agcgccgcgt acaccagata accgccggta gtgtgcagta
     3301 cgccttttgg ttttccggta gaaccggagg tatagagaat aaacagcgga tcttcggcgt
     3361 tcatctcttc cgcctggtgc tgatcgctgg cttgctcaac ctggtcgtgc caccacaggt
     3421 cgcgcccttc ctgccagtca attttcccgc cagtacgctt cagtaccacc acatgctcta
     3481 cgctggtgac gttcgggttt tttagtgcgt catcaacgtt tttcttcagc ggaatactac
     3541 gcccggcgcg cacgccttcg tcggaagtga tcaccagtcg tgagttggaa tcgataatgc
     3601 gcccggcaac cgcttccggc gagaagccac cgaaaattac cgaatgcacc gcgccaatac
     3661 gggcgcaggc cagcatcgca accgcggctt ccggcaccat cggcatataa atcgccacca
     3721 catcaccttt tttaatgccc agcttgagca gggtattggc gaagcggcag acgtcgcggt
     3781 gcagctcttt atagctgata tgtttgctct ggctggcgtc gtcgccttcc cagatgatgg
     3841 cggtacgatc gccgttttct tgcagatggc ggtcaaggca gtttgccgcc agattcagcg
     3901 tgccgtcctc gtaccattta atggacacat taccgggggc aaaggaggtg tttttcacct
     3961 tctggtacgg tttgatccag tcgagaattt ttccctgttc gccccagaag gtatcaggtg
     4021 cgttaataga ttgttgatac atcgcctcgt actgctgagg gtttatcagg caacggtctg
     4081 cgatgttggc aggaatggtg tgtttgtgaa tttgactcat gcttttgttc tccttgtagg
     4141 atgttaataa tatgtggcat aagcgttaaa tgtaggggta ttggcagttt tgtttagtat
     4201 ttgggcgaca gatcacgcaa aagtagaatt gtgcaaataa acggcagggt aatttttgaa
     4261 ggtcagcaac aaaagttgat taattctttg aggaacatgc agttatgcat gctgttgaaa
     4321 aagaggaaga tactgactaa ctctaaagtg gtattttaca tgcacttaca attgattaaa
     4381 gacaacattc acagtgtggt tatttgttac acttaggggc gagcaatgtc atgacagtgt
     4441 aggtgcggtt actgtcgtga aaaacaataa aaaccgccat tgcaacaatg gcgcaattcg
     4501 gatgaagccc ctatgacaag gataaaaata aacgcacgcc gtatcttcag cttattgatt
     4561 ccttttttct ttttcacttc tgttcacgct gaacaaacgg ctgctcccgc aaaacctgta
     4621 actgtggaag cgaagaatga aacctttgcc ccgcagcatc ccgatcaata tctctcctgg
     4681 aaagccacct cggaacagtc agagcgtgtt gacgccctgg cggaagatcc acggctggtg
     4741 atcctgtggg cggggtatcc cttctcgcgc gattacaaca agccgcgtgg acatgccttt
     4801 gctgtgaccg atgtgcgtga aaccctgcgt accggtgcgc cgaaaaacgc tgaagatggt
     4861 ccgctaccga tggcgtgctg gagttgtaaa agcccggatg tggcgcgtct gatccagaaa
     4921 gacggcgaag atggctactt ccacggtaaa tgggcgcgcg gcggcccgga aatcgtcaac
     4981 aacttaggtt gtgccgattg ccataacacc gcctctccag agttcgccaa aggcaaaccg
     5041 gagttaaccc tttcccgtcc gtatgcggct cgcgcgatgg aagccattgg taaacctttt
     5101 gagaaagccg gacgtttcga ccagcaatcg atggtttgcg gtcagtgcca tgtggagtat
     5161 tacttcgacg gcaaaaacaa agcggttaaa ttcccgtggg atgacggcat gaaagtcgaa
     5221 aatatggagc agtattacga caaaattgcc ttctctgact ggactaactc cctgtcgaaa
     5281 acgccaatgc tgaaagcgca gcacccggaa tatgaaacct ggacagcggg cattcacggt
     5341 aaaaacaacg tgacctgtat cgactgccat atgccaaaag tgcagaacgc cgaaggcaaa
     5401 ctctacaccg accataaaat tggtaatccg tttgataact tcgcccagac ttgtgcgaac
     5461 tgccataccc aggacaaagc tgccttgcaa aaagtggtcg cggaacgtaa gcagtcgatt
     5521 aacgacctga aaatcaaggt tgaagatcaa ctggttcacg ctcacttcga agcgaaagcg
     5581 gcgctggacg caggtgcgac ggaagccgaa atgaagccga ttcaggacga tatccgtcat
     5641 gcccagtggc gttgggatct ggcgatcgct tcccacggca ttcatatgca cgcaccggaa
     5701 gaaggtctga ggatgctcgg tacggcgatg gataaagcgg cggatgcacg caccaaactg
     5761 gcgcgcctgc tggcgactaa aggcatcacc catgaaatcc agatcccgga tatctcgacc
     5821 aaagagaaag cccagcaggc cattggcctg aacatggaac aaatcaaggc cgagaagcag
     5881 gacttcatca aaacggtgat cccgcagtgg gaagagcagg cacgtaaaaa cggtctgtta
     5941 agccaataac cccgttccgc ctcgcaaggg gcggaaaaca caatggagtg aatatgagcg
     6001 tattacgttc gttattaact gccggggtgc tggcgtcggg cctgttgtgg agcctgaacg
     6061 gaattaccgc tacccctgcg gcgcaggcat ctgatgatcg ctacgaagtt acccagcagc
     6121 gtaacccgga tgccgcctgt ctggactgtc ataagccaga taccgaaggt atgcatggca
     6181 aacatgcttc cgttatcaac ccgaataaca aactgccggt cacctgcacc aactgtcacg
     6241 gccagccatc accgcaacac cgcgaagggg tgaaagatgt gatgcgcttt aacgagccga
     6301 tgtacaaggt tggagagcag aacagcgtct gtatgtcctg ccatctgccg gaacagttgc
     6361 aaaaagcgtt ctggccgcac gatgtccacg taaccaaagt ggcgtgcgcc agctgccatt
     6421 ccctgcatcc acagcaagat acgatgcaga cgttaagtga caaaggacgg attaagattt
     6481 gcgtcgattg ccacagcgat cagcgcacca atccgaactt taacccggcg tctgttccgt
     6541 tgcttaagga gcagccatga cctggtctcg tcgccagttt ctcaccggcg tcggcgtgct
     6601 ggcagccgtc agcggcaccg cagggcgcgt ggtggcgaaa acgttgaata tcaatggggt
     6661 gcgttacggc atggtgcatg acgagtcgtt atgcatcggc tgtaccgcct gtatggatgc
     6721 ttgtcgggaa gtgaacaagg tgccggaagg cgtctcgcgc ctgacgatta ttcgtagcga
     6781 gccgcagggc gaatttcctg atgtgaaata tcgcttcttc cgtaagtctt gccagcactg
     6841 cgatcatgcg ccgtgcgttg acgtctgccc gaccggtgca tcttttcgcg atgctgccag
     6901 tgggattgtc gatgttaatc cggatctctg cgtcggttgt cagtactgca tcgccgcctg
     6961 tccgtaccgc gtgcgcttta ttcatccggt cacgaagaca gcggacaaat gcgatttctg
     7021 ccgtaagacc aatttgcagg ccggtaagct tcccgcgtgc gttgaggctt gcccgacgaa
     7081 ggcgctgacg tttggcaatc tggacgatcc caacagtgag atttcgcaac tgctgcgcca
     7141 gaagcccact taccgctaca agctggcgct gggaaccaaa ccgaagctgt accgcgtacc
     7201 gtttaaatac ggggaggtga gccaatgacg cagacttccg catttcattt tgaatcgctg
     7261 gtgtgggact ggccgattgc catctacctg tttttgattg gtatttctgc cggtctggtg
     7321 acgctggccg tgctgttacg tcgcttctac ccgcaggcgg gcggtgcaga cagcacgttg
     7381 ctgcgcacca cgctgattgt cggacctggc gcggtgatcc tcggtctgtt gagcctcgtc
     7441 ttccacctga caagaccgtg gaccttctgg aagctgatgt tccactacag ttttacttcg
     7501 gtgatgtcga tgggggtgat gctgtttcag ctttacatgg tggtgctggt gctgtggctg
     7561 gcgaaaatct ttgaacatga tttgcttgcc ctgcaacaac gctggttgcc gaagctgggg
     7621 atcgtgcaaa aggttctgag cctgctgacg cccgttcatc gcggactgga aacattgatg
     7681 ctggtgctgg cggtgttgtt gggggcttat accggttttc tgctgtcggc gctgaaatcg
     7741 tatccgttcc ttaataaccc gatcctgccg gtgctgttcc tcttctccgg catctcgtcc
     7801 ggtgcggcgg tggcactgat cgccatggcg atacgccaac gcagtaaccc gcattccacg
     7861 gaagcgcagt ttgtacaccg tatggaaatc cccgtggtat ggggtgaaat cttcctgctg
     7921 gcggcgtttt ttgtcggtct ggcgctgggc gatgacggta aagtgcgtgc gctggtggcg
     7981 gcattgggcg gcggtttctg gacgtggtgg ttctggcttg gtgtcgccgg gctggggctg
     8041 attgtgccaa tgttgttcaa accgtgggtc aatcgcagtt ccggcattcc tgccgtgctg
     8101 gcggcgtgtg gggccagtct ggtcggcgtg ttgatgttgc gatttttcat tctctacgcc
     8161 gggcagttaa cggtggcgta agccagaaca gaggtggttt ctggacgtat tccttcctga
     8221 agtcggtttt ctggcgctgt tgttaagtct cggggtcaac gtgttgaccc cgttgacggc
     8281 ctttgcggga gtgcggttgc gctggcctgc catgatgcga ctcacttgca tcggcattct
     8341 ggcacagttc gcgctcctgc tgctcgcctt tggcgtactg acgtattgtt ttctcatcag
     8401 cgatttctcg gtcatttatg tcgcccaaca tagctacagc ttgctgtcgt ggaaactcaa
     8461 actggcggcg gtgtggggcg gtcatgaagg ttcgctgctg ctttgggtgc tgctgctttc
     8521 cgcctggagc gcgctgtttg cctggcaata tcggcagcaa accgatccgc tatttccgct
     8581 gacgctagcc gttttatctc tcatgctcgc cgcactgcta ctgtttgtag tgctgtggtc
     8641 cgatcccttc gtgcggatat ttccaccagc aatcgaaggc cgcgatctca atccgatgct
     8701 gcaacatccc ggtcttatct ttcatccacc gctgctttac cttggctatg gcggtttgat
     8761 ggtagcggcg agcgtggcgc tggcgagtct actgcgcggc gagtttgatg gtgcctgcgc
     8821 ccgaatttgc tggcgctggg cgttacctgg ctggagtgca ttaactgcgg ggatcatcct
     8881 cggttcctgg tgggcctact gcgaactcgg ctggggcggc tggtggttct gggatccggt
     8941 ggaaaacgcc tctttattac cctggctttc tgccactgcg ctgctgcaca gtttatccct
     9001 gacacgccag cgggggattt ttcgccactg gtcgctgttg ttggcgatag ttactctgat
     9061 gctgtcgctg ctgggcacct taattgtccg ttctggcatt ctggtttcgg ttcatgcgtt
     9121 cgctctggat aacgttcgcg ctgtgccgtt gttcagcctg tttgcactga ttagccttgc
     9181 gtctctggct ctgtatggct ggcgagcgcg ggacggtggc ccggcggtgc gtttttcggg
     9241 gttatcgcgg gaaatgttaa tcctcgctac gctgttgctg ttttgcgcag tgctactgat
     9301 cgtgctggtg ggaacgcttt atccgatgat ttacggcctg ctgggctggg gacgtctctc
     9361 cgttggcgca ccgtatttta accgcgcgac gttaccgttt ggtctgttga tgttggtggt
     9421 gattgtgctg gcgacgtttg tctctggcaa acgcgcgcag cttccggcgc tgctggcgca
     9481 tgcgggcata ctgttatttg ccgcagggat cgttgtctcc agcgtcagcc gtcaggagat
     9541 cagcctgaat ttacagccgg gtcagcaggt gacgctggca ggatacacct tccgttttga
     9601 gcgcctcgat ctgcaagcca aaggcaatta caccagcgaa aaagcgatag tggcgctgtt
     9661 tgaccatcag caacgcattg gtgaattaac gccggagcgg cgtttttacg aagctcgtcg
     9721 tcagcaaatg atggaaccgt caattcgctg gaacggcatc catgactggt atgcggtcat
     9781 gggggagaaa actggagcgg atcgttacgc ttttcgcttg tatgtacaaa gcggtgtgcg
     9841 ctggatctgg gggggaggat tgttgatgat tgcgggcgca ttgttaagcg gatggcgggg
     9901 gaagaagcgc gatgtataaa gggcttctca cgctactgct gttatttacc tgttttgcgc
     9961 gcgctcaggt tgtagacacc tggcaattcg ccaatccgca acaacagcaa caggcgttaa
    10021 atattgccag ccagttacgt tgtccgcagt gccagaatca aaacttactg gaatccaacg
    10081 cgccggtggc agtcagtatg cgccatcagg tttacagcat ggtggcggag gggaaaagtg
    10141 aagtcgaaat cattggctgg atgaccgaac gctacggaga ttttgttcgc tataacccac
    10201 cgttaacggg tcagacgctg gtgttgtggg cgctgccagt ggtgttgtta ctgctgatgg
    10261 cactgatcct ctggcgagtg agggcgaagc gatgaaacag cctcaaatac cggtgaaaat
    10321 gctgacaaca ctcacgatat tgatggtatt cctctgtatc ggtagttatc tgttaagccc
    10381 aaaatggcag gcggtacgtg cggagtatca gcgtcagcgc gatccgctac atcagtttgc
    10441 cagccagcaa aacccggaag cgcagcttca ggcattgcag gataaaatcc gcgctaatcc
    10501 acaaaacagc gaacagtggg cgttactggg cgagtactat ctgtggcaaa acgattacag
    10561 caattcgctg ctggcgtacc gtcaggcgtt gcaactacgt ggtgagaacg ctgaactgta
    10621 tgcggcgctg gcgacggtgc tttattacca ggccagccag catatgaccg cccagactcg
    10681 cgcaatgatc gacaaagccc tcgcgctgga ctctaacgaa atcaccgctc tgatgctgct
    10741 ggcttccgat gcgtttatgc aggcgaatta cgcgcaagcc atcgaactat ggcaaaaagt
    10801 gatggatctc aactcacccc ggatcaaccg gacacagctg gtcgagtcga ttaatatggc
    10861 gaaattgttg cagcggagat cggattaatc atctggtgat gaagattatg ttattgagct
    10921 gcataaaaaa taatcgaatg aacatatgcc aaaaataatc actaatcagt attattgcag
    10981 attaaacaaa taaaaatctt tccataacaa atggttattc attaatcctg ccataaactc
    11041 gcgttattat gcattaatgc agcgaaaagc tctgttgtta aagggttgcg caacataccg
    11101 cgcaaatgat actgatcata agcgttaaaa aaatctacaa accaacgcaa cacaattcat
    11161 gccctggcag tatgtcacgt tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa
    11221 gtcattcata tgaaaaatat aaaattcagc ctggcctggc agattctgtt tgctatggtg
    11281 ctgggcattc tcctgggaag ctacctgcac taccatagcg acagccgcga ctggctggtc
    11341 gtcaatttgc tctctccggc gggtgatatc ttcatccatc tgatcaaaat gattgttgtg
    11401 ccgattgtga tctccacgct ggtggtgggt atcgcgggtg ttggtgatgc caaacagctc
    11461 gggcgtattg gcgcgaaaac cattatctac ttcgaggtga tcaccaccgt tgccatcatt
    11521 ttggggatca ctctggcgaa cgtcttccag cccggtgccg gggtggatat gtcgcagctg
    11581 gcgaccgtcg atatctcgaa atatcagagc actacggaag cggtacaaag cagttcccac
    11641 ggcattatgg gcacgatttt gtcgctggtg ccgacgaata ttgtggcgtc gatggcgaaa
    11701 ggcgaaatgt tgccgatcat cttcttctcg gtgctgtttg gtctggggct ttcctccctg
    11761 cccgcgacgc atcgtgaacc gctggtgacc gtgttccgct ccatctctga aaccatgttt
    11821 aaagtgactc acatggtgat gcgttatgcg ccggtgggtg tgtttgcgct gattgcagtg
    11881 acggtggcta actttggttt ctcttctctg tggccactgg cgaaactggt gctgctggtg
    11941 catttcgcca ttctgttctt cgcgctggta gtgctgggaa ttgtggcgcg cctatgcggg
    12001 ttaagcgtct ggatcctgat tcgtattctg aaagatgagc tgattctggc gtactccact
    12061 gccagctctg aaagcgtact gccgcgaatt attgagaaga tggaagccta cggagcaccg
    12121 gcgtcgatca ccagcttcgt ggtgccgacc ggttactctt ttaaccttga tggttcgacg
    12181 ctgtatcaaa gtattgccgc tattttcatc gctcagctgt atggcattga cctgtccatc
    12241 tggcaggaaa tcattctggt gctgacgctg atggtgacct cgaaagggat tgctggcgtg
    12301 ccgggcgtgt cgtttgtggt gttgctggca acgctgggta gcgtaggtat tccgctggaa
    12361 ggtctggcgt ttattgctgg tgttgaccgt atcctcgaca tggcgcgtac tgcactgaac
    12421 gtggtgggta atgcgctggc ggtgctggtg attgccaagt gggaacacaa atttgaccgc
    12481 aaaaaggcgc tggcttatga gcgtgaagtg ctgggcaaat ttgataaaac tgcggatcaa
    12541 taattgaaga ttgccgggga tatcccccgg caatgtgtgg atgcctgatg cgacgctggt
    12601 gcgtcttatc aggcctacgt cagacagcgc tagcgtgatt ttgtaggtct gataaggcgt
    12661 ttacgctgca tccgacatca acgcctgatg cgacgcttaa cgcgtcttat caggcctacc
    12721 ttaaccgtta gttaattttt caaactcttc acacccggta tcaaaccctt ccatacagct
    12781 caggttcaac cagtgcagcg ccttttgctt gttcttctcg ataaaacctt cttcaccgtt
    12841 taagaacatc attcccgccc agtactcgga ataaccggtt cgggaaattg cagagctgcg
    12901 tttgaaatac caggttgcct tgtcatcgtc ggccttaatg cccacgccgt tggcgtaaat
    12961 caaaccaagc agcatttggg catcgacggc agagtcgttc tccagatctt ccgaggcgtt
    13021 ttctaacagc gaaatcgcct ttggataatc cggtttaccc gcttgagtat ttaccagaat
    13081 atgcgccagc gttacttcac cttctttact gcccgcttgc gccgctttct ctgcaagaac
    13141 ttttgcttgt ggatagtcca gactgaccgg attggtgatt ttgatctgcg ccaacagcgc
    13201 gcaggcatca gcatcaccgc tgtcggcggc tttctgtgcc caatattcgg ctttgctcaa
    13261 atcgccggag ctaaaccagc tgtcggcaag aaaatattgt gcgcgtcggt ccccggcctc
    13321 tgctgccttt aaatactggc tgccaggctc ggagtcgttg gcgtgggcaa agaatgtcaa
    13381 aaacaacatc aatgcgataa tttttttcat tttgataata cgattgggta gggagtaacc
    13441 agtataaaga gttcgaatgg ataaaaaaac agcctccgaa aggaggctgt agaaaggact
    13501 gtattacgcc agtgccgctt cgcgcaggcg agttttcaac ttgttgtact cgtcaatcac
    13561 gtactgctcg gcggcgcgct gatcggcgat cggctcgacg cgcacggcgc agtatttgta
    13621 ttccggcgtt ttcgtaatcg ggcttaagtt ttcggtaacc agctcgttac aggcaccaat
    13681 ccaccactgg taggtcatgt aaatcgcccc tttgttcgga cgatcgctga cctgcgcacg
    13741 ggtgataatt ttgcctttac gcgagtgcac ccaaaccaat gcctcatctt caatactcag
    13801 acgtttggcg tcttcggtat tgatttgtgc gtagccaggt tcatcagcca gcgccgccag
    13861 tgccgcacag ttaccggtca tcgaacggca agagtagtga ccaacttcac gcaccgttga
    13921 cagtaccatc gggtactcgt cggtgagttt gtcgattggc gctacccagt cgcaggtgaa
    13981 gaactgcgcc agaccgttcg gggtgtcaaa cttctcttta aacagataag aagtcccctg
    14041 atcggcatct gaagtatcgc ggcaaggcca ctgaatgaag cccagttcgc ccattttctc
    14101 gtaagtcgca ccgtagaaat ccgggcacag atgacgcaac tcatcccaga tctcctgggt
    14161 gttgttgtag tgcatcggat aacccatacg ggtggcgatt tcactgatga tttgccagtc
    14221 cgttttcaga tcccatttcg gttcaactgc tttgaagaaa cgctggaagc cacggtcagc
    14281 cgcagtaaac acgccttcat gctcgcccca cgacgttgac ggtaaaataa catccgccgc
    14341 cgacgcggtt ttggtcataa agatgtcctg aacgataacc agttccagat cttcaaaggc
    14401 tttacgtact gccgacagct ccgcgtcagt ttgtagcgga tcttcgccca taatgtacgc
    14461 ggcacgcact tcgccatgcg ctgcgcggtg cggcagctcg ctgatgcgat aaccggtatg
    14521 agcaggcagg ctttccacgc cccaggcttt ggcgaatttc tcgcggttag ccggatcttt
    14581 tacgtactgg tatcccggat acgtatccgg cagcgcgccc atatcgcagg caccctgcac
    14641 gttgttctga ccacgaaccg ggttaacacc cgcatgtggc ttaccgaggt taccggtcag
    14701 catcgcgagg ctggtcagag aacgcacggt ttccacgccc tggtagaact gggttacacc
    14761 catgccccac aggatggcgg cgcttttcgc ctgggcatac atccgtgccg cctgacgaat
    14821 ctcactggcg ctgacgccgg tgatatcttc aaccgactcc ggcgtgtagc cttcaacgat
    14881 tttacgatac tcttcaaagc cttctgtacg ggaagcgacg aatgctttgt cgtacagatt
    14941 ttcttcaata atgacatggc ccatcgcatt caacagcgcg atgttcgagc cgtttttcag
    15001 tgcaatgtgc atgtcagcaa tgcgcgcggt ttcaattttg cgcggatcgc agacgataat
    15061 tttcgccccg ttacgtttag cgttaattac gtgattcgcc acgattgggt gggaatccgc
    15121 cgggttgtac ccgaaaacga acactaaatc ggtattatca atttcgttaa tagcattgct
    15181 cattgcgcca ttaccgaccg attggtgcag acctgcaacc gatgggccgt gtcagacacg
    15241 agcgcagcag tcaacgttat tggtaccaat aacggcgcgc gcaaattttt gcattacata
    15301 gttggtttcg ttacccgtac cacgcgagga gccggtcgtc tggatggcat ccggaccgta
    15361 cttctctttg atggcgctca ggcgctcggc aacgtaattc agtgcctcat cccaggaaac
    15421 aggttcgagt ttgccgccac gctggcgacg gatcatgggg gttttcaggc gcggggtcag
    15481 gatctgggta tcgttaatga agtcccagcc ataataaccc ttcagacaca gggtaccctg
    15541 gttggttttc ccctgcgctg cctccgcccg gacgattttg ccgttatcga cgaccaggtt
    15601 gattttgcaa cctgatgcgc aataggggca aaccgtgacg acttttttca tcggtctcgc
    15661 tccagttaat caaatcacgc atacgcgctc tcgactacag tatgcatctt ttatgccaca
    15721 ttttatttgg ggtcattccc tgatattacg ggcactattt attcaaaact ctgacgaaaa
    15781 acaggctgtc gtcagttttg acgtgacgaa acgaaatacc gcgtgacagc catcacacgg
    15841 cagacatttt attttttctc gacgacggga gccgcctgat acccgctgcc cagcgatttc
    15901 atcaactgaa tgctttggat cacccggcgg ctgtccagca tcagtaatga catctcttcg
    15961 gcaagcaccg gcaaccgggc ttcggtagcc tgtaagcggc tggttaagcc gcgctgatag
    16021 gcggcctcgg cagcgcgctg ggtgaagcgc gttgcttcca cgcgttcagc ctgcatttct
    16081 cgctcgtcgt tgagcgtttg cagacgcgtg ccgttgacgg caacgtcacg caccgcgttc
    16141 agtactgact ggttgtaacg ttcaatcatc atgttgctgg cggcgcgagt gccttcgaga
    16201 ttggcattca accgtccacc gtcaaacagc ggcaatttca gacccgggat gaagttgaac
    16261 tggcgactgg tttttttgaa taaggtatcc aggtggatgg agtccagacc gaaaaacgct
    16321 ttgatatcaa agcttgggta gaacagcgcc cgcgcggaat ccacctgatc taatgacgcc
    16381 tgaacatacc agcgcatggc ttgcagatcc gggcgtctgg cgagcaactc ataagagagt
    16441 gtcgccggaa taccggtctg gactcgtggt aatgccactg gtttgatctc cggcatatcg
    16501 ctggctcccg cgccaatcaa tgcacgcaga gattctcgcg tttcggtgat ttgcccttta
    16561 acggcagcaa tttgtttatc gaccgccaga atctgtgccc gcgcgccgtg gaaaggcact
    16621 tgcgcttcca gaccgtgcgc cactttactt tggtgcgctt tcaccgcgta atcaatcaca
    16681 tcgcgagttt gttctaacag atcgagcatc tgatagctgg cctgcatact gtaataaagc
    16741 tgcgctacgc ccgtggtcag cgatagctct actgctgcgg tttctgccag cgcggcatta
    16801 tgcgcgccaa tggcggcggt aaccgctgag cgatgcacac cccacaaatc aagatccagt
    16861 ccggcaaaca aaccgactgt ggcttccgta tagtacggcc cgtccatacc cagtgctggc
    16921 gcatccatcg cataagggct taaaaagccg ttcgccgaga cgcgttggcg gttgagcatc
    16981 cctaacgctg cgacctgtaa ttgtgaaccg gcatctaaca aatcggcctg ggactgcgct
    17041 ttttcttccc gcagtttcgc ttcggcgagg gtgtgtgaac cacttagcgt ccgttggatc
    17101 agcgcatcca gctgcgggtc attgagttgt ttccaccact gcgcctgcgg ccagccggag
    17161 ctggcaagat gaatatcatc ggccagtttg atttgttccg gtttgagctg ttgatgaggc
    17221 gcagaatcct tgcgtaccag ggcacagccg gaaatcagcg tcgtgctgcc gagaatgctg
    17281 cacaacagca gacgtgaaag ttgacgattg atcattgcgt ggctccttgt gcctgttccg
    17341 tggcgggcgt taatgccggg gctgtccagt ctggcaagcg ggcgaaaagc tggcagacgt
    17401 gttgctcctg ttttaataag gtgggcagga tggcctgcga ggcgggtgtc tcttcaagcg
    17461 ttatttgagg agaacggctg agtgcggttg ccagaccggc agcgtatttt tccagggcgt
    17521 cggcaaactg acctgctttg tccggctgta gtgcgttatc cagcgcctgc gccgagttcc
    17581 aggtggcatc ccaggcgtga agaagatcgc ggccctgatg aataaccgtt tgcgaacgtt
    17641 caatcagtaa ggcgcgttct tcgctgtcca gttgacgctc cagcgccacg cgttggcaca
    17701 tctcttcaca ggcattaaac gccgcatgca gacctatacg aatttgcaga taagtgcgca
    17761 gagccgtgac ttcctgctgg cgtgggatcc gcattacttt acttagcata cccagcgcgc
    17821 cagccagttt ttgcggcagc gtgcgcgctt cactttcagg ccagacaaag gtgtaaatca
    17881 ccgcagacac cacggtacca atgatgatac ccagggcgcg atcgcgaatt tccaccaggt
    17941 cgtacaccgg accaaaaacg ttttcgagcg tggcgagcgc gaaggtgacc accatctgtg
    18001 tgccgatata agaagagcgt tcagagctgg tggcaatcca tgcgcccaac aggaaaatcg
    18061 gtgccagcac aaacagcaat tcgacaatat tgtccagcca gggcatgacc agtagcgtga
    18121 ataacagcgc cagaatcgcg ccgcaaaagg ccccgccaaa acgcagcacc atcttctggt
    18181 acgatgaacc gacattcgga ttagcgacga tcacgcaggt cagcatacag gtgtgaatgc
    18241 cttcccagtc cacgccgctg tagaaggtgt aacagatcaa acaggcgagc agcgttttta
    18301 ccgcgtagcg catatagtct ggattggtaa aagcatcagc gaccattgat ggcggtttgg
    18361 cggcgggcgt tggcggcgta ttcgggtcca tctgacccag ttgtaacaac gtctggcaga
    18421 tattctccag gttacattcc cgtgccgcca tcgcttcact ttcactgatc cgccagtcgc
    18481 tttgccagca ctgaccttcg gtaatggcat gctgcagctt gttgatttct gaagctaatt
    18541 tttgtcggaa ttcaataatt gcctgagaat cagcaaaaga ggtgggatcg tagcgattca
    18601 gcgtcgagta aatgtaggtt accgttgcca cgcagctttg ccaccatgcg ctttgagttc
    18661 gccagttggc atcgtccgcg aggcaaaaga cattgagttt ttgcagcgcc agcgcctctc
    18721 tttcaatccg cgtttcgggt agcggtgcga ggctgtccgt caggtggcta atggcatcat
    18781 caagccgatc attaagcgct tgatgcattt gcgaaatggc acgactggga aaccacagta
    18841 cgccgattaa cgtcattaat aacgtcgggt agaggccaac aacgatacac cacagcgtta
    18901 agcgcacgac cacttccgga tagtcgagca tggcggggaa ggtttgccca taaatggcga
    18961 caatggcgac agcgaaaaag accagtccca accgatgggt gcgcatcaaa aacatgcagc
    19021 ccatcaggat cggcccggcg atgatcaagc ggatcaacgg ttcgccgtat gaccatttgt
    19081 agatcaaaaa caggctgccg atctccagca ccgtggcaac cacaaacaag atcgcgacaa
    19141 atttggtgta aaacgcgttc gactgaatac cgtaaaacag cactgccagc gataacgcca
    19201 caaaagggat ctcaaaagtc atcgagatca gaatcaccag caggcagcct acccagagtt
    19261 gcacggtctg cggcacgcga cctggtcgcc gctcgcttaa ctcttcatga aagaacgcca
    19321 gcagcctgac caccggtaat ggcagggagt tgagcgcgct cattattgcg gctcaagatt
    19381 agcgactgcc gaagcgccga tgcggaacat ttccgggtca ggtttatcaa ccatgatttt
    19441 gaccggaaaa cgctgggcaa cacggaccca gttaatagaa cgagaaactt tcggcaggcc
    19501 gcccagcacc aggccgccgt catccggtag cacgccgtag ccaattgaat ccactttacc
    19561 ctcgaaagtt ttgccgctgt cactcatcag gcgaatcgtt gcaggtgtac ctgagcgaat
    19621 atttttcaga tcggtttcgc ggaagttggc gatcacgtac cagtgacgag tgtcgattag
    19681 ggtaaaaata gggcgcatgg cagaagcaaa ttgcccgacg gaggttttga gggaaatgac
    19741 ccggccatca aacggtgcgc gaacggtcgc catttccaga tgcagtttgg tcagggcaat
    19801 atccgcttcg accgccgcac gctgggcaac taatgcatcc acgccgctga cggcgctggc
    19861 ggctgactgc gcctgcaaca atacagcatt aagatcggct tctgcagcgc gctgcgcggt
    19921 cctcgcacgg tcaacgtctt ccgctgagac aaaaccttct ttcagtaatg gctcggtgcg
    19981 gcgtaatgta tctgtggcct gtttagcggc ggcacgggct ttttctaccg tggcgttaac
    20041 cgagtcggca ccaaactgtt gcgcgtcaac gctacgctgg gtgagcataa tttgcttatc
    20101 cagtgccgcg agggaggctt cagatttcgc cagattggct tcgtacgggc gcgggtcaat
    20161 gcggaacagc aaatcgccct gtttgactgc ctggttgtcg gtgaccgcca gttctacaat
    20221 gcggccgctg acttccggca ccacatcaat ggtatctgct gacacgtaag cgtcattagt
    20281 tgatggcgca ctgtctacgc gccagataac gaaaacaagg gcaaccagcg ccaacgcaac
    20341 cactaacaga gcagggaatt tactgcgagg agcttttttc ggcgtacttt ccatgagcgc
    20401 gtctcaaata gattagaaaa atgccagcca gaacagcatg gcgtagagag caaaaagggc
    20461 ggtataaata attccgacaa atgccagctt gatatttgcc cgctgaataa ctcgcctcgt
    20521 gatgagcgtc aatataaggc tggcaatggc gcagaaaaac cagctgggat aataagcgcc
    20581 gatcaccggg attgccggag ataatgagca tcccgaaacc atgaagacgg gaattatcca
    20641 ggctgttttt ttgagttgca tcgccatgcg agagagaact gttggcatat taaatacatc
    20701 ctgtctgctg cgaacaatat ctgtagtgaa tatcaatatc
//
LOCUS       DAZKIF010000088        20731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZKIF010000088 DAZKIF010000000
VERSION     DAZKIF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.088"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(633..1532)
                     /locus_tag="ACHOTW_003152"
     CDS             complement(633..1532)
                     /locus_tag="ACHOTW_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9845210.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            1631..2353
                     /locus_tag="ACHOTW_003153"
     CDS             1631..2353
                     /locus_tag="ACHOTW_003153"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9845211.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
     gene            2520..2798
                     /locus_tag="ACHOTW_003154"
     CDS             2520..2798
                     /locus_tag="ACHOTW_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845212.1"
                     /translation="MSLYIKLILSIVREISVNTICSLIVVVALSLLSFSSVAKTITAV
                     GSTINSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(3202..3264)
                     /locus_tag="ACHOTW_003155"
     CDS             complement(3202..3264)
                     /locus_tag="ACHOTW_003155"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845213.1"
                     /translation="MNTVVFVATVEYAYLSLHFY"
     gene            complement(3501..4403)
                     /locus_tag="ACHOTW_003156"
     CDS             complement(3501..4403)
                     /locus_tag="ACHOTW_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9845214.1"
                     /translation="MKGNVSMAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSR
                     SVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGT
                     VRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQ
                     GMVAVRITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDD
                     KKITHQPEGQWAFSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRME
                     GLFLYYPHRNVSPALRMVIDTLKI"
     gene            4649..5707
                     /locus_tag="ACHOTW_003157"
     CDS             4649..5707
                     /locus_tag="ACHOTW_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9845215.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            5849..6976
                     /locus_tag="ACHOTW_003158"
     CDS             5849..6976
                     /locus_tag="ACHOTW_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9845216.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKCYPTLMAIPGLMAVIAIGLM
                     LTGHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(7155..8111)
                     /gene="paoD"
                     /locus_tag="ACHOTW_003159"
     CDS             complement(7155..8111)
                     /gene="paoD"
                     /locus_tag="ACHOTW_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFP9845217.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAGLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(8121..10319)
                     /gene="paoC"
                     /locus_tag="ACHOTW_003160"
     CDS             complement(8121..10319)
                     /gene="paoC"
                     /locus_tag="ACHOTW_003160"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP9845218.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQIDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            complement(10316..11272)
                     /gene="paoB"
                     /locus_tag="ACHOTW_003161"
     CDS             complement(10316..11272)
                     /gene="paoB"
                     /locus_tag="ACHOTW_003161"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFP9845219.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYTFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            complement(11269..11958)
                     /gene="paoA"
                     /locus_tag="ACHOTW_003162"
     CDS             complement(11269..11958)
                     /gene="paoA"
                     /locus_tag="ACHOTW_003162"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFP9845220.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            12376..12990
                     /gene="yagU"
                     /locus_tag="ACHOTW_003163"
     CDS             12376..12990
                     /gene="yagU"
                     /locus_tag="ACHOTW_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP9845221.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(13238..13567)
                     /gene="ykgJ"
                     /locus_tag="ACHOTW_003164"
     CDS             complement(13238..13567)
                     /gene="ykgJ"
                     /locus_tag="ACHOTW_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9845222.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(13880..14590)
                     /gene="ecpE"
                     /locus_tag="ACHOTW_003165"
     CDS             complement(13880..14590)
                     /gene="ecpE"
                     /locus_tag="ACHOTW_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP9845223.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            complement(14559..16202)
                     /gene="ecpD"
                     /locus_tag="ACHOTW_003166"
     CDS             complement(14559..16202)
                     /gene="ecpD"
                     /locus_tag="ACHOTW_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFP9845224.1"
                     /translation="MRVNLLIAMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDTSGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNQGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            complement(16192..18717)
                     /gene="ecpC"
                     /locus_tag="ACHOTW_003167"
                     /gene_synonym="yagX"
     CDS             complement(16192..18717)
                     /gene="ecpC"
                     /locus_tag="ACHOTW_003167"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP9845225.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGTLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIATSGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            complement(18743..19411)
                     /gene="ecpB"
                     /locus_tag="ACHOTW_003168"
                     /gene_synonym="yagY"
     CDS             complement(18743..19411)
                     /gene="ecpB"
                     /locus_tag="ACHOTW_003168"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP9845226.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            complement(19469..20056)
                     /gene="ecpA"
                     /locus_tag="ACHOTW_003169"
                     /gene_synonym="matB"
     CDS             complement(19469..20056)
                     /gene="ecpA"
                     /locus_tag="ACHOTW_003169"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP9845227.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            complement(20131..20673)
                     /gene="ecpR"
                     /locus_tag="ACHOTW_003170"
                     /gene_synonym="matA"
     CDS             complement(20131..20673)
                     /gene="ecpR"
                     /locus_tag="ACHOTW_003170"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP9845228.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
BASE COUNT         4736 a         5307 c         5426 g         5262 t
ORIGIN      
        1 tattgtctat aaaatgtagc gatgcaattt tattgcaacg aaatgatttt tacaacatac
       61 cattatcgct aaacaaaatc gcacccatca aatcaactta cgttacaatc actacaaagc
      121 gattgcattt aaattcaaaa cagttttctg ttgggcgcat tttttgttgt actggactaa
      181 agagaaaaaa tggggtaagg aacaaatacc ttccgtgctc aaaaattagg tttttatgca
      241 cttattaatc agtcaaggtg aacagtaaag tgttcacttc ttatcaatcg ttaatttcat
      301 atctttttga atattaatga aaaaaaacac aatgagtagt atgaacagtt ttcccccaaa
      361 aaaagttttt tctgccttga gaatgcgcgg aaagcgaacg ccttcaccaa tgtgtatagg
      421 ggtgtgtgta gtttaaatct tctattttaa tttatgtatt aaaatcattg tgttggtggc
      481 gattattggt tcctattatc gcaccattta aatcaagtag ttaccccata tttaaataca
      541 ccacgtttcc tcctgtgccg catttgtgcc attgtaaccc tggcaattaa tcaaaatact
      601 gttctgacat caggcaatgc aggtgcagac atttaagcca attgctgccg ccattcctta
      661 acgtggtcaa tcagagcgcg gagctttggt gcaatattgc gacgctgtgg gaaatacaga
      721 tagaagcccg gaaattgtgg aagaaagtca tcaagcagag atacaagctt accgctttca
      781 atatacggcc tgaaagtttc ctgagtggca attgttattc ctccgccggc aagagccagc
      841 ctcaacatca gacgcagatc attagtcgta atctgcggtt caatcgcaag gtcgaaagct
      901 ctcccgtttt cttcaaatgg ccagcgataa ggcgcaacct ccggggactg acgccagccg
      961 atacacttat gattcacaag ttcacgcgga tgaacaggtg tgctgttggc tgcgagatag
     1021 gagggagagg caacgaccat ttcacgctgc ctgccggtaa ggggcacagc aatcatgtct
     1081 ttttcaatga cctctccgag ccttacgcca gcatcgtaac ctgcggccac gatatcgaat
     1141 tcctcatccg tgacaacaat atcaagcgta acggctggat tggctgctgc aaacgaagcg
     1201 attagcggac ctgaaagaaa ttcttcagct attgacgtca ccgcgattct gagaagtccg
     1261 cgtggcacat ggtcagaaac catctcttcg aacgctgcct caataccgga taaaggtaat
     1321 gacagcgatt tatgcagccg ttctcccgct tctgtcaaat taactgagcg cgtggtccgc
     1381 attacaagca ttgtcccgaa tgcatcttca aggcgtctta ttccctgact gaccgctgaa
     1441 cgggtaacgc ccagccgtgc ggcggcttta ctaaaattac tctcttctgc taccgcgata
     1501 aaaatgggta aaagattaag atcgattttc attgtttaat gccgctaacc actgagtaca
     1561 ttgaaagcag gatactggaa ccaatcataa agttctattc tctctccgaa cacatttgaa
     1621 ggagaacgta atggataagg tcatattaat taccggcgca tcaagtggta ttggggaagg
     1681 tattgccaga gagcttggaa tgacaggtgc aaaggtttta ctgggagcac gcagagttga
     1741 gcgtatcgaa gccattgcaa ccgaaatctg ccgcgcagga ggaattgcta aagcgcggga
     1801 gttggatgtc acagaccgac agtccatggc cgatttcgtg caagcagcgc tggatagctg
     1861 ggggcgagtt gatgttctta tcaataatgc gggcgttatg ccgctttcac cgcttgcagc
     1921 aggaaaacaa gatgaatggg cgctcactat tgacgtgaat atcaaaggtg tactgtgggg
     1981 aattggcgct gtacttccgg tgatggaagc acagggttcc ggtcagataa tcaatcttgg
     2041 ttctattggt gccctttctg ttgtgcccac aggcgcagtc tattgtgcat caaaatttgc
     2101 agtacgggct atttctgatg gcttacggca ggaaagctcg aaaatccgtg tgacctgcgt
     2161 aaatcctggc gttgtcgaaa gtgaactggc ttcaaccatt acccatgtgg aaaccatgaa
     2221 ggcaatggac gcctaccgtt ctgttgcact tcaacccgcg gatattgccc gtgctgtaag
     2281 gcatattatt gaatctcccg aaagcgttga tacaaccgaa attaccatca gacccactgc
     2341 atctgcaaac tgaagaataa cttttacatt cacgttttat taaataggtc agaaggaata
     2401 tttatatatc cctgtgtttt tcatagcaag atacattatg tattgctcac cccgaaggag
     2461 atgaaggttt aattacagaa agattatttt tcacaggaag taaacatgac tcatgtttaa
     2521 tgtctttata tataaaactc atcctctcaa ttgtcaggga aattagcgtg aatactatct
     2581 gttctttaat tgttgtggtt gcactgtctt tattatcatt cagtagcgtc gccaaaacga
     2641 ttactgccgt cggttcaacc attaacagca ctgaaaaaga aatttcttta caggcagaaa
     2701 aacaagggaa atcatataaa attctgggcg cgttttttaa gaacagagtt tatatgatag
     2761 caaagttaac accagtcagt aaaaatgatg cttcataagg ctcatggtat aatttttaag
     2821 acatgatcaa gaagcacgtc tattgactca aaattgtcat ttgtcaggca atggaatacc
     2881 tgacaaatga cgctatccca atctattgta ccagtcgttc tgctaattct accgatgctt
     2941 cggtctggag tggtagagta atattggaca cagttttatt ttctctcttt ttcgagggct
     3001 gtatatcaac aggaagaata catccctaag cggttaatgc tggagtaatc aatagaccag
     3061 cattaatcac agatgattac caatcagaca gaaatggtta aatcgtcgaa ttcatattta
     3121 atacgttttc ttacgccgct gaacagcttg aaaaggatgc atattttaaa tatcagtgaa
     3181 atgaacagtt tattgccgtt attaatagaa atggagggat aaatacgcgt attctactgt
     3241 tgcaacaaaa acaacggtat tgatcggttt tcataatgaa gtaacctgac agatatgaaa
     3301 atgttgattg aggctaaatg cccggattaa ggttgtcgag gggagccagg tggttttttc
     3361 ttttggatga tctccattgc tggatagtcc ccttgtacag ataagtccgc tctttctcgc
     3421 tcaaagcggc ccctggaatt gaacgcagag tgactacctg acacaatatt gcacagtgaa
     3481 gagtcggtcg ttgttaacgt tcagattttc aatgtatcaa tgaccatccg cagagcgggg
     3541 gagacgttgc gatgagggta atagagaaac aatccctcca ttcgcaggct gtaacgctgc
     3601 aacactctga taagcttacc gtgttctaaa tcatccgcga ccagttcaac aggaacatag
     3661 gccaacccga ggcccagccg ggcggcttct gcttccatat agctgtcgga aaaggcccat
     3721 tgtccttcgg gttgatgagt gattttttta tcatcctgat tgagttccca ctggtatacg
     3781 ctgccgtcgg caaactgata ggcgatacag ggatgcgcct ttaaatccgc cggggtttgc
     3841 ggaaaaccat agcgacgaaa atgttccgga gtccccacaa ttgccatctc catatcgggc
     3901 gtgatgcgta ccgcgaccat gccctggctg acttcaggcc ccaggcggac acctgcatcg
     3961 aatctttcct cgatgatatc gacgaaccgg ctctcattta tcagttcaag ctggatatca
     4021 ggatatcgct gtttaaatac cgccagcttt ggcagcaggc atttatcaat cgcgtgctgg
     4081 ctggcattga tacgcaccgt gccggagggc gtctctcgat agtgcgccag cgtggcaagc
     4141 ccaagatcta acatttcaaa tccagatgcc gtcttctggt agagctgctc gcctgcttgc
     4201 gtcagcgaca gtttacgcgt ggtgcgcacc agcagctgga cgcccagcct ttcttcaagc
     4261 tcgcgaacag aacggctgac tcctgactga gcaagaccca gccgcttagc cgctgcggta
     4321 aagctgcctt cccgcaccac ctgcataaac aggtatagct cgttgtagtt ctcccttttt
     4381 gccattgaaa cattcccctt catgccggca atctcagcat taatttataa caatatggta
     4441 ttaatcttag cattaatcgc cccctaatca gcactaattc tgctcactat aatgacccca
     4501 tcaaagtaaa gcaccgcagc gttgtccgtc ggaaacctgt atctctctgt tgttttttat
     4561 ggggattttt atgaaagcct tcacccgaac gctaaaaacc gcgatgccgg tcatgctgct
     4621 tttcgcatca ttaagtggag tcacgacaat gagttatgct gattcaacca atcctaatgc
     4681 ccctgtgtcc atgaccgata aatgggataa aacgttcgcc gagagccaga aagtcgatca
     4741 ccgtaaagtc tcgttcccga accgatatgg catcacctta gtgggcgatc tttacctgcc
     4801 taaagaccgt ggcgatcgca agctggcggc gattgcggtc agtgggccat ttggcgcggt
     4861 gaaagagcaa tccagcggct tgtatgcgca gacgctggcg gaacgagggt ttgttaccct
     4921 ggcgttcgat ccttcttaca ccggggaaag cggcggctat ccgcgaaatg tcgcctcacc
     4981 ggatatcaac actgaagatt tcagcgcggc ggtggatttc ttagggctgc aaaaagaggt
     5041 agatcgcaac cgtatcgggc tgctcggtat ttgtggctgg ggcggcatgg cgctgaacga
     5101 cgcggcgatg gatacccgcg ttaaagcggt tgctaccagc gtgatgtatg acatgagccg
     5161 ggcgatgggc catggtgtgg gggatggcga agaccgttat tctaccgccg accgtcgtgc
     5221 tgttctgcaa tatttgaatg aacagcgctg gaaggatgcg cagagcggaa cgttcgctca
     5281 cggtggtcat gatattaatg tcgatagcaa cggcaaggtc agtgcgggcg agcgcattct
     5341 gccagaaacg ctgccggcaa atcctcatcc ggtactgaaa gagttctttg actactatcg
     5401 catgccgcgc ggcttccacg aacgttccgt taactctacc ggggcgtgga cggcaacgat
     5461 gccgctgtca tttatgaata tgccgctgtt gagctacgcc agtgaaatca ccattccgac
     5521 gctgattgtg accggtgaga aagcacattc acgctatttt gctgaagatg cttacaaggc
     5581 ggttggcagt aaagacaaag agttggtggt ggtaccaggg gcaaatcacg tggacctgta
     5641 cgataacgtg gccggaaaaa tacctttcgc gaagttcgaa caatttttcc agaccaaact
     5701 gaaataaaac ctcccctgaa ttgatgcaaa gccaccggtc tatggccggt ggcttttatc
     5761 ctgatctttg tgaaatttca ctatgtcgac actaagccaa acaccatcac ctcatcatca
     5821 gcacgcatac tggggcggta tttttgccat gaccctgtgc gtgtttgtgc tgattgcttc
     5881 tgaatttatg cccgtcagcc tgctcacgcc gattgcccgt gacctgggcg tgacggaggg
     5941 gctggcaggg cgggggattg ctatctccgg tgcgctggcg gttctgacca gcctgacgct
     6001 ttcaacgcta gctggaaaga tgaatcgtaa gttcctgctg ctggggatga cggttctgat
     6061 ggccgtatcg gggcttatta ttgcgctggc taccagttat ctgatgtata tggtcggtcg
     6121 tgcgatgatt ggtgtggcca ttggcggatt ctggtcgatg tcagcggcaa cggcgattcg
     6181 tctggtgccg cagcatcagg tcacgcgtgc gctggcgatt ttcaacgccg gtaatgcgct
     6241 ggcgacggtc gtggccgcgc cgctgggaag ctatctgggg gctacggtcg gatggcgagg
     6301 cgctttcttg tgcttggtgc ccatggcggt tgtcgccttt atctggcagt gcattagtct
     6361 gcccagcatg gatgcgaata aaagccacac gtcatgtggc gcggtattcc gcttatttag
     6421 ccgccgcatg gttgtggtcg gcatgatagc ttgtggcctg tttttcatgg ggcagtttgc
     6481 gctattcacc tatgtgcgcc catttctgga gtcggtgaca cgggttaatt ccttaggctt
     6541 gtcgttgatt ttgctgatta tcggcgtggc cggttttatt ggcacgctgg ttgtttcgat
     6601 atttctcaac agaaaatgct accccacgct gatggccata cccggactga tggccgtcat
     6661 tgccatcggt ctgatgctta ccgggcatca cgtctggatg gtctcgctgc tgttaggact
     6721 ctggggcatg ctggccaccg ccgcgccaac cggatggtgg acatggattg cgcgtacgtt
     6781 gcctgacaac gccgaagccg gaggcgggct catggtcgcg gtgattcagc tctcaatcgc
     6841 ccttggatca acggcaggag gcatcgtgtt tgaccgcctt ggctggcaga gtacgtttgc
     6901 catgagtagt gtgctgctcc tttgcgcggg cgtactgacc tttgttacag cacgccagaa
     6961 ggccggtgcg ctttagtccg gcgagggcgc gcgttgctga aaaagttaca caaggcgatg
     7021 agcaaccact atgggtaggg cgctctttta aacgatttag gtgattttct aaaacggcta
     7081 ctgcaaattt ttctcttaat ttatggtatt tcccccggag gtgagaaagc gctttccacg
     7141 cccggccgca aggatcagga cgacgggggc aggcatgaat cctcctcctg atggagacgt
     7201 acagaggcga cttctgccag cacggagagg gccagtgtat gcgcattccg ggctttgggg
     7261 aatatcccga cgggtgcccg gatttgcgct gtctcctccc tggaccatcc cagctcgtgg
     7321 agcttttgca gacgtaacgt gtgggttcga tagctgccca atgcgccgag ataaaagggt
     7381 tttgcttctc gcgcggcctg caacactggc agctcccggt tgagatcatg gcacagcaaa
     7441 atgaccgccg tatcggtatc gatctgagcg ctggctgagg ccggaaaaag atcgaagata
     7501 tggctgtcat agcctgtggc tgctgcaaga ctcgcggttg cctgcgcctc aagagaacgt
     7561 ccgtaaatca tcagcctgac gcatggcctg aaccccacct caaagccatt gagattccag
     7621 cccgttcggg tttgcgtggg caggcacacc aacgattgtg cttgcggatc gtagcgcagc
     7681 cccgccggtt ttctctgttc caggcggttc agcacggcga gcagaggctg tgccgagcgt
     7741 agtttatgga gcgtcagcgt gatcccaccg ccgcagggca gaacgatgtc aaaccacggc
     7801 gaaccttcgc catagcgaat ttcgcgatcg cagcctgagc ccatcatctc cagcgcttca
     7861 aaagcggcag cggcctccac gcagccgcca gagacaaaac cgcagtaacg accatcttcg
     7921 cgcaccacca tctgcgcccc gagcgggcgc gccgcgccgc cgcgtatctc caccagcgtc
     7981 accagccccg ctcctttacc ggacattagc gcctctacgg cgaagcgcag aatggtccgg
     8041 tggtcatcgg tgagaaacgc ctgctctggc ttatgccagt gttcgtcttt atcaaaaagc
     8101 gggtatgaca ttgttcctcc ttaaaccaca tccggcagct tatcgagcag cttatccaga
     8161 gtgatgggat aatcgcgtac ccgaataccg gtggcgttat acaccgcgtt ggcgatagcc
     8221 gcgctcacgc cgcacaggcc cagctcaccg acacctttgg ccttcatcgg ggaggatatg
     8281 gggtcggtat catccaggaa aatcacttcc tgttttggga tatccgcatg gaccggcacc
     8341 tcataccccg ccatatcgtg attaacgaag tagcccaaac ggtcatccac cgccagctcc
     8401 tccatcagtg ccgcgcccat gcccatagtc attgcgccaa tgacttggct gcgtgcagtt
     8461 ttcggattca ggatgcgtcc tgcagcacac acagcgagca tacgccggac ccgaacttct
     8521 cccgtcgcgc tatgcacgcc gacctccaca aaatgcccgg caaaggtcga ctgctggtac
     8581 tccttgctca gtgttccgaa ttcaatgctc tcttccgctg tcagtctgcc gcctgcggtg
     8641 gcctcatgta gtatggcgct tcgggtaccg ttggtaatct tgccgtcggc aaactgcgac
     8701 tgctcaggat caaacccgac tgccgaggca atcatttcgc gaagcttcac acaggcggcg
     8761 taaacgccgg aggtggaggt attcgcgccc cattgtccac cagaacccgc agaaaccggg
     8821 aaactggaat cgccgaggtg aaccgcaacc tgctccagcg gtacgccaag catttccgct
     8881 gccgtctggg ccagaatggt gtagctgccg gtgccaatgt cggtcatgtc cgtttccacg
     8941 gtaacggtgc cgtttggttc gaggtgaacc cgagcccccg atttttccag cagattattg
     9001 cgaaagcccg ccgcgacgcc gtggccgact agccactccc cgtcgcgcac ctgtccgggt
     9061 gtggcgttgc gctgcttcca accaaattta tccgctccgg tgcgcaagca ctcgataagc
     9121 tgacggcgag agaagcggcg cgtcgggtcg gcggggtcaa tctgagtgtc attcagaatg
     9181 cgaaactcga cgggatcgat gcccgctttt tccgccagtt cgtcgatcgc gatttcgagc
     9241 gccatcagac cgggggcttc gccgggcgca cgcatggcgt tcccttccgg caaatcaagc
     9301 gtggcgagcc gcaggccggt atgacggttt gccccggcgt agagtaattc gctttgctgt
     9361 accgccgttt ccggcgtgcc gccgggcagg tttccagacc agctttcatg tgagatagcg
     9421 gtgattttcc cgctctggtc ggcaccgata cgcaagtgct gaagggtggc ggggcggtgc
     9481 gtggtgttat tgggaataga ggggcggggg agcatcactt taaccggacg tttcaccgct
     9541 cgggcggcga gggccgccag cagcgcatcg cttctcagga acagcttgcc gccaaaccct
     9601 ccgccgatat acggggagat aatacgcaca ttctccacgg gcactttcag cgtttttgcc
     9661 agatcggtgc ggcaccagtc aatcatctga tttgaggtcc agagagtaag cttatttcca
     9721 tcccagacgg ccatcgaggc atgcggctcc atcgccatat ggctctggtc cggggtcgtg
     9781 taggtagcat caatcttcac cgcagcggag gagaaagccc cgtcaaagtc accgacgttc
     9841 ttgtcgggcg tgtcttccgg cggctgactg acggcctgtt tttcgtccgc cagggagtaa
     9901 gctcctttat tacggcgata gtgcgcctgc accagcgagg ccgccgctcg cgcctgttcg
     9961 aaggtctcgg ccactaccag agcaatggcc tgatgatagt gctcaatagt ggggccgcct
    10021 aacagcctgg cggtgttttt gtcgccttta ctgagtgccc cggcgttact ggcggtaatg
    10081 acaggcagta cgcccggcgc tttttgcgcg gcgtccgtat caagggcggt gaggcgtcct
    10141 ttggcaatgg cggaaccgac gatatagcca taggcggcgt tgggggattc ttcatgccat
    10201 tcgtaggcgt agcgtgccgt gccggtagtt ttcagcggtc cgtcgatgcg gtcatgggga
    10261 cgaccgacaa ccttcagctg atcgatcggg ttttcccctg cgggtttatc aaatttcatg
    10321 cttgtgccct cgcttcagcc agtacggagg caagcgttcg cttcgccaac aggagtttaa
    10381 aggtgttttc agcggtggga tgggcgctgg cgaacagcgc gtcatatacg gcctgcgccc
    10441 cctgggatag ctgagcatcc gcagcctcaa tgcgccaggg cttatgtgct actccgccca
    10501 gcgcgacgcg cccgctgccg tcaggctgaa taatcgccgc gaccgatacc agggcaaagg
    10561 tgtaggaggc gcgatcgcgc accttacggt agatatgttt tccgccgagc ggcggaggta
    10621 acgtcaccgc aacgataagc tcaccgggaa gcagggcggt ttcaatgtgc ggcgttttcc
    10681 ccggtgggtg ataaaaatca gccagtgtga tactgcgagt ctttccctcc ggcgtgatgg
    10741 tttccaccac cgcatccagc aaccgcattg cgaccgccat atcgctcgga tgggtggcaa
    10801 tgcaggcttc gcttacgcct accaccgcgt gctgacggct aaagccttca agcgccgcgc
    10861 agccgctccc gggcaggcgc ttattgcagg gctgattggt gtcgtaaaaa taggggcagc
    10921 gcgtgcgctg gagcagatta cctgctgtgg ttgcctgatt gcgtaactga ccagacgcgc
    10981 cagcgagcag ggcgcgggag agtaccgcgt aatcacgacg cacgcgctcg tgagccacca
    11041 ggtcggtgtt ccgtaccagt gcgccgatgc gcagcccacc cgcgtcggtc acttcgatct
    11101 tatcgagccc gagcccgttc acatcgataa ggtgggtggg cgtttcaatt tccagcttca
    11161 tcaggtccag cagattggtc ccgcccgcga taaattttgc gccgggtacg cgctgagcgc
    11221 taagtgccgc ctcggcgggg gtgttcactc gttcataggt aaacgccttc atgattttat
    11281 ctcccccgca gcgtcttcaa tggcggcaag gatgttagcg tatgcaccac agcgacagat
    11341 gttgccgctc atacgttcac ggatctcatc ggcagttctt tctggagggg aaaccaaatc
    11401 gaccgtgacg tgactgggaa tgccgtcctg aatctctttt agcaccgcta ctgatgagca
    11461 aatttgcccg gaggtgcagt agccgcactg gaagccatca tgcttgataa aggccgcctg
    11521 catggggtga agattatctg gtgaacccag gccttcaatg gtggtgatct cggccccctg
    11581 atgcatgact gcaagcgtca ggcaggcatt aagcctgcga ccattgacca gcacggtaca
    11641 ggctccgcac tgtccgtgat cgcaaccttt cttggtaccg atcaaatgca gattttcacg
    11701 caaagcgtcc agtagcgtgg ttcgggtatc cacctcaagc tgctcggttt tgccgttcac
    11761 cttaagtgtc aggggcatta tctcaggcgc gggtgtagct gccggaacgc ttgccgcaag
    11821 cgtagaatga ggataaacca cggcggccgc cgccgttgcg gcgctcactt taatcagatc
    11881 gcgacgggtc aaacttaaat cgtgcggctc atgcttccca acccgattat cttcggggta
    11941 ttcgccttgg ttgctcatac caggcctccg gtattctaaa ggggaaaata agagtgcgtt
    12001 gcgagagtgc tgatgctttt ttaaacgtta agcatagtcg gcgacagcag gaggattatt
    12061 cttaaaacgc gaatgcagct atgagcccgg ctaataaatt catgttcgcc gggatgttga
    12121 tgatgatggg agctggttta ttgctttagt tgtacgatgc aaaaaccgat aaggaaacct
    12181 gtgattgtca gctctgcatc accctgcaaa tctatgtcac ttctaatata aaaataggga
    12241 gaaatgatgg tgcttatatt ctttggtgat tagacaacca tcttgttata caaaacaata
    12301 cagttcttta catttacctt gttttatgaa tactcctaaa gaggtgtata acataatggt
    12361 acaagcaggg tagatatgaa tatatttgaa caaactccac cgaaccgcag acgttatggt
    12421 cttgctgcat tcattgggct gattgctggc gttgtttccg catttgtgaa gtggggggct
    12481 gaagttccat tgccgccacg tagcccggtg gatatgttta atgcagcgtg tggcccggaa
    12541 tcattaatca gggctgctgg gcaaattgat tgctcgcgta attttctcaa tcctccgtat
    12601 atttttcttc gagactggct ggggctgaca gatcccaatg cggctgttta tacctttgcc
    12661 gggcatgtct ttaactgggt tggtgttacg cacattatct tttcgatagt gtttgctgtc
    12721 ggttattgtg tggtcgcaga agtatttcca aaaattaaac tctggcaggg cttactggca
    12781 ggtgctttag cccaactttt tgttcatatg atttcattcc ctctcatggg actgacacca
    12841 cctctgtttg atctcccgtg gtatgagaat gtttctgaaa tttttggaca tttagtctgg
    12901 ttctggtcca ttgaaattat tcgcagagat ttacgaaaca gaattactca tgagccagac
    12961 cctgagatcc ctttaggctc aaacagataa tgcattgaat gataaaaatg gcgcaaatac
    13021 agcgccattt ttataggtta aaaacattgc tttttatatt ctgattcaga tagtcagtga
    13081 gtatatcgcg ctacttcagg atgatgtaga tccgaagaac gctacagaag agaggcattg
    13141 ttgctggcaa atagaagaag tatcggggtt tgttacccct gaaaaacgaa gccccgctat
    13201 tacagctggc ggggcagtgc aaataattat tgtatattta taacggtgtc agcccgtatt
    13261 tagcccttgc acgattgcaa gcctcattga cgactccgtt ctcaccagac atggcgaatt
    13321 ctctgcatgt ggacgatcga tttttatata tcgtgcagca ggcatttttg cccggggttc
    13381 ctgcaagggc aatacatcgg gggtttttct gattagttcc gctcatgcat cggtgaaaag
    13441 gggatatttg ttcagtgagc ctggcgggaa tagtgccgcc agcatcgtcg gcttcggccc
    13501 agtagaaaga gacgcggaaa aatgcacaac aggcaccaca cgtcatgcat ggattcagat
    13561 tgctcataat tcaccagtac agctataaat cgtaaagaga actgcggtac gtcgtatgca
    13621 gggacgtatc gcggttggct ggtgtacttt cgatagtgtg agtattgaat gatttccagc
    13681 cgttaccgat tttacgtcct aattagtgaa gaaaccactc gttagcaggc cgcctggtac
    13741 ctttccgtgt ctcctgaaca ctgtggtgtg catatgagaa agcctatcgt tacaggtgat
    13801 attgacgatc gcttccacat catttaaacg atttttgagt ctctgaggtg gaattcttcc
    13861 ctcgaccgat ggataagtat cagtccgccg aagggggctt agccggacag gaatcgctaa
    13921 tcttaatgaa tttgtcgtta tagaccagat agtgattccc cggctgacgt aactcaggct
    13981 gatgaaccac gtcttccgga cgtagatacc aggcatcgcc ttcctcttcg gtcgaatcac
    14041 atcctggttt aatcagtagc ttaaaccacg tgttgccggt gttacttacc gttccggtca
    14101 cctgatcgaa ggaccattta aactgaacct gacgtggtcg tactaccaga atggtatcca
    14161 tcaccaccac cggctccgtg ctaacctcac cgccggtagg gctgcgtttt gtcaggttac
    14221 gagtggggac ctcgcgaaat gagacccggt agtagcgctc gcggttatcc tgtggcccat
    14281 gatagtaaaa tttaaaatac tcgctctcac cagcctgcaa cgccagctgg cggggggcga
    14341 aaagcagttc accatccacc ggtcgggtgc gtaattcact gctgcccggg ctatcgatag
    14401 cactgatggc aatacggtat atccgcgcgc ttttgttgtt attcactaca cgtttgctga
    14461 caaagtcagt ctcggacggc agtgaaaaag tcagattgcc gaccgaaatc gcctgtgtca
    14521 aaggtgccgc cagcatacag accgctgtca gcagggcctt agttaatgtt acgccacgtc
    14581 gcctgaacat gaatttctcc tgaagcgctg acttcgccaa accagccgtt atcatctacc
    14641 gtacgcagag aaatggcgtt atccattgga atcgaaaatt tgactgtggt cttatccatc
    14701 tgccccactt cgccagagat atcagtccac ggtgtggtca accacagcgc atcggtcata
    14761 tcccgccagc tatcatcgca cccggcatcg taggtttttg tagctccgtt gcgggtaata
    14821 aaggaaagcg tcgccgggaa cggtactttc gccttaccgt catcggagct gaagacacaa
    14881 taggagcgcc cgccaatcac ctgcgcgggt ccggtcacct tgatcagcac ttcgtcggca
    14941 gctgttttac cgctggtggt tacgatatag ccgaaatcga gtgccgactc gccgctgccg
    15001 acatatccct cccgcgacgg tgtctgagta tattcatcgg agataatact gatgctgaaa
    15061 tcacggggtt tgataatcag cgtgttggag gtagaaaatt cataccagcc agactccggt
    15121 gatgtggtgt tctgaaagcg aaagttgaat agatctttgg tgttgatatc gctgattccc
    15181 tggttcacca tctgcttaaa gaagttgctc gagaagaaaa catagatcga gtctgcgctc
    15241 ttcatctcgt tgaaggtgta gtaataggca gtattgctca ccggtttcca tgaactgcca
    15301 ttcagactga actgcatatc gtacgccccg acggccgagg ctaacgacga gttggcgatc
    15361 gccgggaata tatggattga ggtgttgctg agtccgtttg tttgcagggt atagttaacc
    15421 atcttacaac ttaatcctga acggctgccg atggtttgcg tccggcagtc ggcgttccct
    15481 tcgcccagag tcggtactcc gtcgctgttg ataaatactt ccgccagcga gtgggtattt
    15541 atcaaccgta gatttgctgc tttcgtatgg gtgacgttgc gaacatacca gttgccggag
    15601 gcctgatcct tacagcgtgc gccgctgctg gcgtcatagt tcacagaggt ctggcaggca
    15661 ttgatggtca ttgtaaagct gctgccgacg ggcatttgtt gcagatactg gtaaaacgcg
    15721 tctgacaaca tgccgtgcat ccacttcgcg ccgccgctgg tcaccgtcgc gccataaaat
    15781 ccactggtgt cggtggtttg cggcaggata agactggtgg tcatattaca cccagcgtac
    15841 cagttgatgc agcgcaagcc agttaaagga gatgaaactg gtgaattttc cagccacata
    15901 tcaaacttcc agttggtata aaggccggtg ttgtaaccgt tatcaatgta gccgaggctt
    15961 tgctgataga tggttcctga accattgtat tttaaaccgg tccagcggtt ggcaccggtc
    16021 aggcgcggat ccagcgcccc gccaggagtg acgaaaaagt tgtcgtctga gttgttttcg
    16081 acaaacacaa actcgcgtgc cggtgcatcc gcccaggttg ttttgctcac tgccgctctg
    16141 agcgcagtta ctggccagat tagcgcaaag attatcatcg ctatcagtag gttaactctc
    16201 attctcttct cctgtctgcg tcaccgccgc ccacgatgag aggccgctgc agaccacatc
    16261 accgacccag acggcaccgc gcgcctggtt gagttccagt gccacttcgc aggttttatt
    16321 gccactgtag cgaaaatcga tagtggggta tttcttatcc acgtccatga caaactcgcc
    16381 gttttcatcg gttcgggttc ggccgatatg gttgttaatc cgtgcgttag ccagcagtgt
    16441 gccgtcttcc gcacggatac gaccggagac ggtaaccatc tgcttcacct ctggctcaat
    16501 gacagcgaca ttgcctggat agagagtcag atgacttttg cgaccgctga cgatatcgta
    16561 actgtcgagt gagtttttgc tgttctgtaa ctccacctca tatcttccat agggagagag
    16621 cgggagatag ttacgcttgc cgttaagcgg gaaaatccgc ccgttgattt tggcgctgat
    16681 ctgaccgtcg tcctccagcc cggtgttgaa tatcacccca gcgttgccat cagtccgccc
    16741 gctggtagca atgtttttac cctgccagcc gacgctgcca ttggcggtta aattggtatt
    16801 gacgtagccg tccgccgcgc tattgacgtt cagcgttccg ctggcgtagc gagcgtcgaa
    16861 ctgtgcatac gccccaccac tgagagtttt gtcatcaccg gtatcgccgg agatggctcg
    16921 tgacagattg gcaccaacag tgcgaatggt cccttcatca aactgcttgc gtgctgacag
    16981 gtttgccatg gtgtagccgt tttgatgagt catccctgcg ctaaaccagt tgcccagtgg
    17041 tagcgagaga tcgagagcga tatatttccc tgtattggcg ttgctgtcgc cgttgttata
    17101 gcgctgaata ccggcccgca ggccaagcga accaaaggta ccgctgtaga cattttgata
    17161 gtaatctgcc gtgtaataat ggctgttgta acggcggtca tcattgtagc tgatgctgaa
    17221 tgtgcccagc ttcgaccaca gtgagttcag gttgagtgtg ccgccgatag cacggttgtc
    17281 ggcatcgcta cgtcgcaatt gattgccaat gcgggttttt tcctgattaa cccacagcga
    17341 actaaagcct cccggtagag tggcgctgat gctgccgatg ctgctccatg agctgtcact
    17401 ggccagcata ttttgcaggt taacgttgat cgctccccca agcggcagcg tcagacgggt
    17461 ttcacccacc gcctgattat cgtatccata tcccgttgcc gcccagctaa gcgtactcag
    17521 tgagccggag gtcgaggcac ctgccagcca actctcttta gctggtcgcg tctttttccc
    17581 gttttccgac cagcgatcca tatgaaagct accgccccat acctgccacg ccagtggtgc
    17641 accgacgccg cgcccccggc taaacagctt attgacccgc tgggtgcgtt tgctgatcac
    17701 gcgaccgtta acgatcacct caacttccac atcgtaaata ccgtatggta gaccccgggt
    17761 atccacttca tgattgccca tggtgaagtt ctgaacgctt agtaaccgcc catcacgggt
    17821 gagatgtact tcacccgccg ccggtaaaaa ggcgatcact ggcgtggctg actggctgct
    17881 gtcgaagatg gtggagctgg cctggtttcc ccaggaaagg ccgtaaatct tccctgctga
    17941 aatggcggtc atcggcccta aggactgcaa gttccaggtg tcgagcgttc caccggcaaa
    18001 tcggtgaccg gcaaaatcgc gttcatacat cgctttatat aattcactgt cctgttgacc
    18061 gctaccgatc ccgtacagcg agccgtcgag caccacatga tgttcgcgca gtgcagtaac
    18121 gttattcagc gacagatagc tggatgtgtt gctcccgccg ttacgcaact ggttgttata
    18181 gatgccgaag ttatagctca gattactgct gagggtgtta acactggact gcccgatgtc
    18241 ttcgctacgt gagcgtagta cagtgcccag cgcttcgcgc ttgaccacca gctgcagcag
    18301 cagttggcgc aagctgagat ccagctgcgc gttgtcagtc agggggatgg taagggcctc
    18361 attgaacggg gcgttcgcca gagccatcag ctgctgtcga gtttgttcgc tgacactggc
    18421 gttatcttca ctctcttcca gctgtatttt ccggatgcgt agctgtccat catccaacca
    18481 gataaaagcg ctgccgattc gctgatcgtc ctggcgaccc tggctaccgg cgagatgaat
    18541 atagagcggg acgctcatgc cgtcctgaag cgcctgactg aaggcctgcg gaataatcac
    18601 cccacctatt tgctgcgcac ttatgtcagc agcgctggca tcgggctgaa cgaacaaaaa
    18661 gaccatgccg aaggcaaact gggctttcag tcctggggag aaccgtcgta aaggcattgt
    18721 cgttatccat ctgcacagct atctatttca cgggaatgaa cttatcaccc tgccaaagtg
    18781 caacccgtcc tttgttatcc gccgtgtcca cgcgggtaaa acgacgcgac ttgcccggca
    18841 tcaggtagta attctcttta cactctttac cattggcggc tttcaggcag gggccgtagg
    18901 cgaggatccg cagcgtcgca tttcctgtat ttgtcagggt gccgttggcg tactgaaagt
    18961 ggtagtttgc ctggcggggg gcgacgacca gaatggtgcc gatgcgggcg gaagcagtgg
    19021 ccacagcgct gcggttggca ttatcgcgct gcgcatcact gagggcctga tcaaaccaga
    19081 caatgcggta gtagcgctct ttttcatctg ctggtccctt atagaagaag cggatcactt
    19141 cgctggcttg ggcgggtagc agcaagctgg cgggagtgag tagcaactcg tccggcttgt
    19201 ccattgagat aacctgcccg tcgtcaagcg gtgaagagag ccgttcgaga cggatattga
    19261 taaggcgacc actgtcggta ctgtttttga tcgttttgct cagcgtgctg ctgtcactgt
    19321 tcataaacga tgatatatcg ccgacatcca gcgcctgggc cggagatatt ccggataaca
    19381 gcagagcgag aagcagaagg tgctttttca taacaattcc gtccaggaat aaagctgggg
    19441 ggctacccct gctggtacat cagagagatt aactggtcca ggtcgcgtcg aactgtacgc
    19501 taacgtcgcc gctccagatg ccttccggta gagtgctgta atcggttact gcggtggtac
    19561 cattggtggt gccgctgatg atggagaaag tgaaaccatc ctgtgcggtg gtacgattgc
    19621 tggcattgta accgtttgcc agcgggctaa ggttgccgcc cagtacgccg ttggcggtat
    19681 cgatcatcac ggtatcgcca gttttttcga ctgccgcgcc gttataatcc acgcccacat
    19741 tcagtgtgga acctgaggta tccaactggg ttaatgtgtt ggtgataaga cgtgaggtca
    19801 gtttaaaggc ggtagccgtt gagtcaccct cgatagccac gtcaaataga cctttctgtg
    19861 agttaaaacc tttaatgcct tcggcatact ggaacgccag gctaccgagt ggcgtcacaa
    19921 ccagcttact ggtggtgtct tttttggctg ttgctgacca ggtcgctaca gcctgagctg
    19981 ttacgtcagc agcctgcgcc acacccatgc cggtaaacac cgttaccaga gctattgcca
    20041 gaaccttttt tttcattgta tttcttcccg agttgaattg aggacgtgat gtcccagtga
    20101 gtcatttttt aaaactaact tgcctggagt ttactgaacc aacttatata attttgagta
    20161 cagcttggcc tctgcattac gccgatgggt atacactgtc tttacactac aattttcaat
    20221 tctggcaatc gatttaggca gcattccctg agccgtcatg cggataattt ccatttcccg
    20281 gtcagtgatt ttatctttct taatatcttt tctcaggaac ctgccattaa tcacataggc
    20341 cagttctttt ctaatatcac gactcagtcc agcgtatacc acgcccctaa tattgctgtt
    20401 gtaataccaa taatttgcta aggcttctga ctttctggcc gcaatcaata cgacctgttt
    20461 cccctttacc gcggataacc actcattatc acggtttata aattcagtta aagaatccgt
    20521 attgagattg ataaacacga aatcttttct gattttctcc agcgatagat aaattaatct
    20581 gtcgatatcc acaatcagtt cagatagtcc tttatagaag taggcgtcat ggggagacca
    20641 gatgtattta tcagaacggt tttgacattc catatggttt tcaacctcat agtccctgct
    20701 gtaatcattt tgccatgtca ctactttcca a
//
LOCUS       DAZKIF010000089        20461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZKIF010000089 DAZKIF010000000
VERSION     DAZKIF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.089"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            118..1173
                     /gene="phoE"
                     /locus_tag="ACHOTW_003171"
     CDS             118..1173
                     /gene="phoE"
                     /locus_tag="ACHOTW_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP9845229.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1212..1613)
                     /gene="crl"
                     /locus_tag="ACHOTW_003172"
     CDS             complement(1212..1613)
                     /gene="crl"
                     /locus_tag="ACHOTW_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9845230.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1671..2915)
                     /gene="frsA"
                     /locus_tag="ACHOTW_003173"
     CDS             complement(1671..2915)
                     /gene="frsA"
                     /locus_tag="ACHOTW_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9845231.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(3007..3465)
                     /gene="gpt"
                     /locus_tag="ACHOTW_003174"
     CDS             complement(3007..3465)
                     /gene="gpt"
                     /locus_tag="ACHOTW_003174"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9845232.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3726..5183
                     /gene="pepD"
                     /locus_tag="ACHOTW_003175"
     CDS             3726..5183
                     /gene="pepD"
                     /locus_tag="ACHOTW_003175"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9845233.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5240..5761)
                     /gene="prfH"
                     /locus_tag="ACHOTW_003176"
                     /pseudo
     CDS             complement(5240..5761)
                     /gene="prfH"
                     /locus_tag="ACHOTW_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5760..5963)
                     /locus_tag="ACHOTW_003177"
                     /pseudo
     CDS             complement(5760..5963)
                     /locus_tag="ACHOTW_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(6282..6734)
                     /gene="yafP"
                     /locus_tag="ACHOTW_003178"
     CDS             complement(6282..6734)
                     /gene="yafP"
                     /locus_tag="ACHOTW_003178"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9845234.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(6744..7142)
                     /gene="yafO"
                     /locus_tag="ACHOTW_003179"
     CDS             complement(6744..7142)
                     /gene="yafO"
                     /locus_tag="ACHOTW_003179"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFP9845235.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(7145..7438)
                     /gene="yafN"
                     /locus_tag="ACHOTW_003180"
     CDS             complement(7145..7438)
                     /gene="yafN"
                     /locus_tag="ACHOTW_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFP9845236.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(7490..8545)
                     /gene="dinB"
                     /locus_tag="ACHOTW_003181"
     CDS             complement(7490..8545)
                     /gene="dinB"
                     /locus_tag="ACHOTW_003181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9845237.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVTPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8616..9401)
                     /gene="lafU"
                     /locus_tag="ACHOTW_003182"
     CDS             complement(8616..9401)
                     /gene="lafU"
                     /locus_tag="ACHOTW_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP9845238.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            9373..11085
                     /locus_tag="ACHOTW_003183"
                     /pseudo
     CDS             9373..11085
                     /locus_tag="ACHOTW_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(11230..11727)
                     /gene="rayT"
                     /locus_tag="ACHOTW_003184"
                     /pseudo
     CDS             complement(11230..11727)
                     /gene="rayT"
                     /locus_tag="ACHOTW_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(11903..12652)
                     /gene="yafL"
                     /locus_tag="ACHOTW_003185"
     CDS             complement(11903..12652)
                     /gene="yafL"
                     /locus_tag="ACHOTW_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9845239.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            12862..13122
                     /gene="dinJ"
                     /locus_tag="ACHOTW_003186"
     CDS             12862..13122
                     /gene="dinJ"
                     /locus_tag="ACHOTW_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFP9845240.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGV"
     gene            13125..13401
                     /gene="yafQ"
                     /locus_tag="ACHOTW_003187"
                     /pseudo
     CDS             13125..13401
                     /gene="yafQ"
                     /locus_tag="ACHOTW_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
     gene            13557..14297
                     /gene="dpaA"
                     /locus_tag="ACHOTW_003188"
     CDS             13557..14297
                     /gene="dpaA"
                     /locus_tag="ACHOTW_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP9845241.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14268..15035)
                     /gene="yafJ"
                     /locus_tag="ACHOTW_003189"
     CDS             complement(14268..15035)
                     /gene="yafJ"
                     /locus_tag="ACHOTW_003189"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP9845242.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(15140..15718)
                     /gene="lpcA"
                     /locus_tag="ACHOTW_003190"
     CDS             complement(15140..15718)
                     /gene="lpcA"
                     /locus_tag="ACHOTW_003190"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP9845243.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            15958..18402
                     /gene="fadE"
                     /locus_tag="ACHOTW_003191"
     CDS             15958..18402
                     /gene="fadE"
                     /locus_tag="ACHOTW_003191"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP9845244.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(18445..18891)
                     /gene="ivy"
                     /locus_tag="ACHOTW_003192"
     CDS             complement(18445..18891)
                     /gene="ivy"
                     /locus_tag="ACHOTW_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP9845245.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19072..19842
                     /gene="yafV"
                     /locus_tag="ACHOTW_003193"
     CDS             19072..19842
                     /gene="yafV"
                     /locus_tag="ACHOTW_003193"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP9845246.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(19960..>20461)
                     /locus_tag="ACHOTW_003194"
     CDS             complement(19960..>20461)
                     /locus_tag="ACHOTW_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HFP9845247.1"
                     /translation="TQEISHGRKETRLHIVSNVTPEFCDFEFEWKGLKKLCVALSFRQ
                     KKEDKSAEGVSIRYYISSKDMDAKEFAHAIRAHWLIEHSLHWVLDVKMNEDASRIRRG
                     NAAEIISGIKKMALNLLRDCKDIKGGVKRKRKKVALNTCYIEEVLASCSELGFRTDKM
                     KNLTQI"
BASE COUNT         5030 a         5191 c         5175 g         5065 t
ORIGIN      
        1 tttaagaata tcattaatct gtaatatatc tttaacaatc tcaggttaaa aactttcctg
       61 ttttcaacgg ggctctcccg cagaatattc gcgcgttaat taaaatcagg aatgaaaatg
      121 aaaaagagca ctctggcatt agtggtgatg ggcattgtgg catctgcatc cgtacaggcc
      181 gcagaaatat ataacaaaga cggtaataaa ctggatgtct atggcaaagt taaagccatg
      241 cattatatga gtgataacga cagtaaagat ggcgaccaga gttatatccg ttttggtttt
      301 aaaggcgaaa cacaaattaa cgatcaactg actggttatg gtcgttggga agcggagttt
      361 gccggaaata aagcggagag tgatactgca cagcaaaaaa cgcgtctcgc ttttgccgga
      421 ttgaagtata aagatttggg ttctttcgac tatggccgta acctgggcgc gttgtatgac
      481 gtggaagcct ggaccgatat gttcccggaa tttggtggcg actcctcggc gcagaccgac
      541 aactttatga ccaaacgcgc cagcggtctg gcgacgtatc ggaacaccga cttcttcggc
      601 gttatcgatg gcctgaactt aaccctgcaa tatcaaggga aaaacgaaaa ccgcgacgtt
      661 aaaaagcaaa acggcgatgg cttcggcacg tcattgacat atgactttgg cggcagcgat
      721 ttcgccatta gtggggccta taccaactca gatcgcacca acgagcagaa cctgcaaagc
      781 cgtggcacag gcaagcgtgc agaagcttgg gctacaggtc tgaaatacga tgccaataat
      841 atttatctgg caacttttta ttctgaaaca cgcaaaatga cgccaataac tggcggcttt
      901 gccaataaga cacagaactt tgaagcggtc gctcaatacc agtttgactt tggtctgcgt
      961 ccatcgctgg gttatgtctt atcgaaaggg aaagatattg aaggtatcgg tgatgaagat
     1021 ctggtcaatt atatcgatgt cggggctaca tattatttca acaaaaatat gtcagcgttt
     1081 gttgattata aaatcaacca actggatagc gataacaaat tgaatattaa taatgatgat
     1141 attgtcgcgg ttggcatgac ctatcagttt taatgattat tgccggatgt gatgcatccg
     1201 gcacatttca ctcacgccgt taacttcacc ggctcgtcac gaaaatcatc cgccggttcc
     1261 aacttcagat tcagcgtcgt cagcagttca cgcagcttct cgtgaaactc acgcagggtg
     1321 tgctccagtc gttcaaccac ttcagtatct tttaccggaa cactcttcca gtcgcctgct
     1381 ttatcgaaca gaccaaactg gtaactgtag gtaaaacggg attcctgcgc ttcaagctcc
     1441 atccaccagc cccagaactc acgcacttcc ggtgccggtt tcacgttgac gcatacagcc
     1501 agacaatcga aaaagaatcg attatcttcg cacttacctt cacgaatata cgggcctagt
     1561 acggtaaatt ttttgatcaa tctgctcttc gggtgtccac tcggtaacgt cattgctatc
     1621 tcctgttgtg atgcaactgt tttaccaaat tggcaaaatt tagcaaattt ttaacacaag
     1681 cgtttttcga tccagtcggt gatttcctga agacctttgt caaaattccg atacaccggg
     1741 ttaaatggga tctctaataa tttaccgtca gcagatgatg aggtgattaa gcgtgagtcc
     1801 tcttccgggc tgaacggatc gttcttccag tagcctgata acattggcgt tgggcatcga
     1861 cgtcccagca atccttgcac ttttaatgaa tagcgattca gctccacgcg caacgcttcg
     1921 tcggaagcat catgcatccc caaacgactc gccagaacgt caagatacat ttccggcacc
     1981 tgttgctggc acttaaaatc actcaacagg gtatgaacta ccggaccaag acaggcaacc
     2041 gctttcagac gcggcgattc aagatatgcc agacgcacgg caacgttagc gccgaaacgg
     2101 aaaccaaagg ccgcgacgcg agtgtgatcc acccacggta cgttaggcag agcctttaag
     2161 acgcgctgat gcaacaggca ggagtcctgg gtgagcttcc attttgaaga aaagcccacc
     2221 gacggcatat caatagtcag catcgcaatg ccgcgcggcg caaaataacg ttcatacagg
     2281 ctgtaatagt ccgtctgcat cgcatccaga ccaccacaca ttaataccgt cgggaacggg
     2341 ccatcgcctt tcggcatatg caaaaagccg gtgatgggcg caccgccggg tacggtaaac
     2401 tccatctgtc gcatcgtgcc cggtagacgc tgagcggcct cttcataggc gcggtttgac
     2461 aaagcctgcg cttgctcggc caggtcatct cctttcagat gaggataggc ggcaatgttg
     2521 tacaacgtag ccgcatgcag ccagtgacga ccactgagtt gcggatcttc ttccgcacag
     2581 gctttttgtt gccacaccat cgcctgggtg gcccactcat aaatccagtt gccgccacgg
     2641 tagccaatca ccgtatcgta taaatcatcg tcggtacgtt cggcatcgct catcacaata
     2701 cgtgcctgga cgtcgaggat ttcgcgtggg tcaatgccgc gccagatcca catcagacgg
     2761 ttaatcatgc gataccagtg agggatggtt ttaccatcaa gggccgactg cacaggcggt
     2821 tgtgcgccgt gattaaagcg gcggactagc gtcgaggttt caggatgttt aaagcggggt
     2881 ttgaacaggg tttcgctcag gtttgcctgt gtcatggatg cagcctccag aatacttact
     2941 ggaaactatt gtaactcgcc tgaagttaaa aagaacaacg cccggcagtg ccaggcgttg
     3001 aaaagattag cgaccggaga ttggcgggac gaatacgacg cccatatccc acggctgttc
     3061 gatccaggta tcttgcggga tatcaacaac ataatcatca accagcggac gaccagccgg
     3121 ttttgcaaag atggtgacaa agtgcgcttt tggatacatt tcacgaatcg caaccgcagt
     3181 accaccggta tccaccaggt catcaataac gatgaagcct tcgccatcgc cttctgcgcg
     3241 tttcagcact ttaagctcgc gctggttgtc gtgatcgtag ctggaaatac aaacggtatc
     3301 gacatgacga atacccagtt cacgcgccag taacgcaccc ggtaccagac cgccacggct
     3361 tacggcaata atgcctttcc attgttcaga aggcatcagt cggcttgcga gtttacgtgc
     3421 atggatctgc aacatgtccc aggtgacgat gtatttttcg ctcatgtgaa gtgtcccagc
     3481 ctgtttatct acggcttaaa aagtgttcga ggggaaaata ggttgcgcga gattatagag
     3541 atctggcgca ctaaaaacca gtatttcaca tgagtccgcg tctttttacg cactgcctct
     3601 ccctgacgcg ggataaagtg gtattctcaa acatatctcg caagcctgtc ttgtgttgac
     3661 aacattttct gctaaccctg tgacctgcaa tactgttttg cgggtgatcg acaaggagac
     3721 ttaacgtgtc tgaactgtct caattatctc cacagccgct gtgggatatt tttgccaaaa
     3781 tctgttctat tcctcacccg tcctatcatg aagagcaact cgctgaatac attgttggtt
     3841 gggcaaaaga gaaaggtttc catgtcgaac gcgatcaggt aggtaatatc ctgattcgta
     3901 aacctgctac cgcaggtatg gaaaatcgta aaccggtcgt cttgcaggcc cacctcgata
     3961 tggtgccgca gaaaaataac gacaccgtgc atgacttcac gaaagatcct atccagccat
     4021 atattgatgg cgaatgggtt aaagcgcgcg gcaccacgct gggtgcggat aacggcattg
     4081 gtatggcctc tgcactggcg gttctggctg acgaaaacgt ggttcacggc ccgctggaag
     4141 tgctgctgac catgaccgaa gaagccggta tggacggtgc gttcggctta cagagcaact
     4201 ggttgcaggc tgatattctg attaacaccg actccgaaga agaaggtgaa atctacatgg
     4261 gttgcgcggg gggtatcgac ttcacctcca acctgcattt agatcgtgaa gcggttccag
     4321 ctggttttga aaccttcaag ttaaccttaa aaggtctgaa aggcggtcac tccggcgggg
     4381 aaatccacgt tggcctgggt aatgccaaca aactgctggt gcgcttcctg gcgggtcatg
     4441 cagaagaact ggacctgcgt cttattgatt tcaacggcgg cacactgcgt aacgccatcc
     4501 cgcgtgaagc ctttgcgacc attgctgtcg cagctgataa agtcgacgcc ctgaaatctc
     4561 tggtgaatac ctatcaggag atcctgaaaa acgagctggc agagaaagag aaaaatctgg
     4621 ccttgttgct ggactctgta gcgaacgata aagctgccct gattgcgaaa tctcgcgata
     4681 cctttattcg tctgctgaac gccaccccga acggtgtgat ccgcaactcc gacgtggcaa
     4741 aaggtgtggt cgaaacctcc ctgaacgtcg gtgtggtgac catgactgac aataacgtag
     4801 aaattcactg tctgatccgt tctctgatcg acagcggtaa agactacgtg gtgagcatgc
     4861 tggattcgct gggtaaactg gctggcgcga aaaccgaagc gaaaggcgca tatcctggct
     4921 ggcagccgga cgctaattct ccggtgatgc atctggtacg tgaaacctat cagcgcctgt
     4981 tcaacaagac gccgaacatc cagattatcc acgcgggcct ggaatgtggt ctgttcaaaa
     5041 aaccgtatcc ggaaatggac atggtttcta tcgggccaac aatcaccggt ccacactctc
     5101 cggatgagca agttcacatc gaaagcgtag gtcattactg gacactgctg actgaactgc
     5161 tgaaagaaat tccggcgaag taattatttg atttgctgcc ggatggcgtt taatcgcctt
     5221 ccggcagttt catccttcat tatccttcga taaaagccat ccctgtgaac gttcgtcgcg
     5281 ggttgccacg ttcaatctgg tgatggaaca ttcgccgctg cgatttcagc actgcgctat
     5341 tttcctgttg ctgctgctcc agcttccagg caatcagcaa tcgcgccagc cgtttgttag
     5401 catgctggct gcgttctgac tgaaccttca cgctaatccc ggttgccaga tgcgtggcgc
     5461 gtaccgccga gtcggtttta ttgacatgtt gaccgcccgg ccccgacgga cgcagcgtct
     5521 cataacggat tgcatccgat tgttcctgct catcagcggt aaaacgccca atgcccagaa
     5581 accagttttt gcgcccatga tgaggccgat acggactcag acaaatccac tgaatagtgc
     5641 cgcaccaact ttcgcttaac gcccatgcgt tgtcgccatc cagagaaatc agcgccgaac
     5701 gcagcgtgtc agagtagcgg cccgtttctg tttccagcac cgttaccgcg acgtcttgtc
     5761 gggtaaaaac gaccaacaga gaagaacgct gcgccaatcg ggtagccgcg tccggggtgt
     5821 aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt gtgtaattgt
     5881 tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac cgcatcggat
     5941 aagggacgaa tatatttgcc catgtaccat tccatttata ctcgtcatac ttcaagttgc
     6001 atgtactgcg actgcgttcg ttcaccccag tcacttactt atgtaagctc ctggggattc
     6061 actcgcttgt cgccttcctg caactcgaat tatttagagt atataaatat aaaaatcagg
     6121 aaatggcaga cgaatccagg gcaagaaaaa aaagaattaa tcctggaaag gtctgcaaag
     6181 agtcattgat aaaggatttg tcatgattta cctccttgca gtttgtggaa taagaaaacg
     6241 cgcacatacc aggcgggcgt tattttcatt gcaagctgga tttaatgttg cggtttatat
     6301 cgcatagaaa aattagtaaa ccacgctccc cggcattcaa cgcgctgctg cttaactgtc
     6361 tgaaaaccat aacgttcaaa aaagggtttt gcggttatgc tggcgtccac cgtaagttcg
     6421 gattcagact taatccaagg ttttaacaaa gcgctggcaa ccccacggcg ggtgtattca
     6481 gggtcaacaa ataacatatc gatataatgt tcaatgcggg aaataaaacc aaccggttgt
     6541 gcattaatga ctgcaacccg cacttgtgat ttcgcgagtt tctccttcca gcgagattcg
     6601 tcaatctgcg cccaggcggc aatttgttgt ggtgaataat gctgactggc ggtcatcata
     6661 accgctctaa tgaaaatagc gcatagttgc tgaaaatcgc caggctgata gtttcttatt
     6721 tgtatgttat tcataatata aattcaaaaa cgcatgcgaa acgcttctgc cattttccca
     6781 attttatgca tttggttgtt atctcgagcc agtttatgag gttcaggttt cagaatggca
     6841 atgagcaacc atgcttgctc atcaaacgca ccctgacaat ataccaaatg cgattcgtca
     6901 ttcgttctgc tgaattggcg caactgtggc ggaaatggat tattcacatt tgccagatga
     6961 atatgagcaa ctcgctcaaa tttgattaat ggccaagtaa acgagtcgtc gtagagtgca
     7021 tcgcgaccaa atatatctgg caaaacaccg tcacgcttat aggaaataaa atccgccgtt
     7081 aacgcatcaa gctcctctgc tgtaagttgc aggcgaataa gttttgtttt gaatacccgc
     7141 atccttattc cttaaagtca ttgaaatcat catccgtcat atcattaaga tatttttcag
     7201 ctcggcgtgt aatttcctct aatcttgcag cacttggacg gaagcgcgcc tttatgggct
     7261 ttttctcctc ttgttcagca agcatgtcca ttaacgcttc gaatgcgctg gcacttaaga
     7321 gatatcctgc ggggcgatta ttagaaagaa ccgcaaccgg ttgatcaata aagtatttag
     7381 ctgggttttt acgtaattca gtgatattga ccgatttttc agcgagaatt cgatgcatac
     7441 agtgatcccc tcataataat gcacaccaga atatacataa tagtatacat cataatccca
     7501 gcaccagttg tctttccatt tgagggtcaa gcaacgtcac atgcagcccc accagtcgca
     7561 caccgcgccc gccgcggcgt tcatcccagg ttttacgcgc ggtggcgatt aagtcagctt
     7621 tattcagccg cggccagacg tgctcctggg tggtttgctg aaaatcgtcg aactttaatt
     7681 tcaccccctg gcgagcaatc agtaaatcag gttttacctt tgccagacgg cgttcaagtt
     7741 ccggatacag ccgctcgata atcgcttcac attcagacca gtgatgaata tcttccgcca
     7801 tcgtgcgttc cacgccgacg gattttcgta accgttcgct gttaacgtcg cgctcgtcaa
     7861 tcccctgact acgctcccac aaaatgcggc caaatttgcc aaagcgttta agcagaatca
     7921 ccagatcaca cttttgtaca tcaccgcagg tccgcagccc catcgcttcc agctttgccg
     7981 ctgagacttt gccaacaccg ggaatttttg ccagcggtaa ggtttgcaga aatgccgaaa
     8041 cttctgccgg cgtaatcaca aactggccgt tgggtttatt catgtcggag gcgattttgg
     8101 cgagaaactt tactggtgtc acgcccgcag acgccgtcag ttgcagctcg ctgaaaattg
     8161 tctggcggat ttcctgggcg atgagggtcg cagaaccgtg gcaatggacg ctatcagtga
     8221 catcgaggta ggcttcatcc agtgacaacg gttcaatacg cgaggtgtag cgcgagaaga
     8281 tttcacggat atgatttgag gcttctttgt aggcgtcaaa gcgccccgga agcaaggtga
     8341 gatgtgggca taatttgagc gccatccctg tcggcatagc gctgcgtacg ccaaatttac
     8401 gcgcgggata attggcggtg ctgatcaccc cccgacgttc gcggctgccg ccaatagcaa
     8461 tagggatatc gcgcagggcg ggattgtcgc gcatctccac tgcggcgaaa aagcagtcca
     8521 tatccacatg aatgatttta cgcattactc acctctcaac actggtaaag tatacagtga
     8581 tttcagggtt tgagaaatgc gtaaagattc agcatttagc gcgttcgctg cgaaagcacc
     8641 tgctgcttat ccagcttttg caccagcggc tgcaccactt tatccccatg ctgaccaaag
     8701 tattgataca gcgtatcgga cgcacttttg gtcagcacca taatctcaat gcgtcggttg
     8761 cccgcgcttt gcggattttt cgcgtccagc agcatctggt ccgccattgc gcttacctgc
     8821 atcactttat cttccggcat tccggcctct tccagcaccc gacgagctga aagcgcgcga
     8881 tcgcccgaaa ggttccagtt gttgtagata ttgtttttgt aggccatcgc atcggtatgc
     8941 ccggtaataa taattttgtt atcgagcgag tcgaacactg gcgcgagctc caccagcaaa
     9001 gttttaaaga acggcataat ctgcgcgctg ccgcgttcga acatattgcg attctggtcg
     9061 tctttaatca gcacgcgtaa tccctgcggg acaatctcca tctccagatt cgcttccata
     9121 tgcgcatcac gggcaatggt gttgatactg gttgccagct cgccaagctc ggtggcggat
     9181 ttcttcttca gcagcgccgt tttctcattc acatcacgcg cttttttctc cgtctcttcc
     9241 ggcaccgcca cggtggcagg ttttggcgga tgagacgggc tgattttatt taacggcgac
     9301 agcccaccac cgttaaaaat cgactgcccg tgcagcgcgg cgataatgct ttcacgctcg
     9361 gatttactga cgctgttgac aatcaacttt atcgtcgtca ccaaaggggc cgagcgtatt
     9421 tccgaggttt ctgcccgctt taccctggat gcgatgcccg gcaaacagat ggcgattgac
     9481 gccgatctta acgccggatt gatcaaccag gcacaggcgc aaacccggcg taaagatgtt
     9541 gccagtgagg ccgatttcta cggcgcgatg gacggggcat cgaagtttgt gcgcggggat
     9601 gccatcgccg ggatgatgat tctggcgatt aacctgatcg gcggcgtctg tatcgggata
     9661 tttaaataca acctgagcgc cgacgccgcc ttccagcaat atgtgctgat gaccatcggc
     9721 gatgggctgg tggcgcagat cccttccctc ctgctctcca ccgcagcggc gattatcgtc
     9781 acccgcgtca gcgacaacgg cgatatcgcc catgacgtgc gccaccagct gctggcaagc
     9841 ccgtcggtgc tctacaccgc caccgggatt atgtttgtgc tggcggtggt gccgggaatg
     9901 ccgcacctgc cgtttttgct gttcagcgcc ctgctgggtt ttaccggctg gcggatgagc
     9961 aaacagccgc aggcggcgga ggcggaagag aaaagcctcg aaacgctgac ccgcaccatc
    10021 actgaaacca gcgagcaaca ggtcagttgg gaaaccattc cgcttatcga gcctatcagt
    10081 ttaagcctcg gctacaagct ggtggcgctg gtcgataaag cgcagggcaa cccgctcacc
    10141 cagcgtattc gcggcgtacg gcaggtgata tccgacggta acggcgtgct gctgccggag
    10201 atccgcattc gggaaaactt ccgcctcaag cccagccagt acgccatttt catcaacggc
    10261 attaaggccg atgaagcgga tattccggcg gataaactga tggcgctgcc ctccagcgaa
    10321 acctacggcg agattgacgg cgtgctgggg aacgacccgg cgtacgggat gccggtgacc
    10381 tggatccagc ccgcgcagaa ggcgaaggcg ctgaatatgg ggtatcaggt gatcgacagc
    10441 gccagcgtga ttgccacgca tgtgaacaag attgtgcgca gctatattcc tgatttgttt
    10501 aattatgatg acatcacgca gctgcataac cgcctgtcgt cgatggcccc gcgcctggcg
    10561 gaagatttga gcgcggcgct gaattacagc cagttgctga aagtgtaccg tgcgctgctg
    10621 accgaaggcg tttccctgcg cgatatcgtc accatcgcca ccgtgctggt cgccagtagc
    10681 gcggtgacta aagatcatat tctgctggcg gccgatgtgc gcctggcgct gcggcgcagc
    10741 attacccatc cgttcgttcg caagcaggag ctgacggtgt atacgctgaa taatgagctg
    10801 gaaaatctgc tgactaacgt agtgaatcag gcgcaacagg gcggtaaagt gatgctcgac
    10861 agcgtgccag tggacccgaa tatgctcaac cagttccaga gcacgatgcc gcaggtgaaa
    10921 gagcagatga aagcggcggg gaaagacccg gtgctgctgg tgccgccgca gctgcgccct
    10981 ttgctggcgc gctatgcaag gttgtttgcg ccggggctgc atgtgctgtc gtataacgaa
    11041 gtgccggatg agctggagtt gaagataatg ggggcgttga gttgattggc ctttaagggg
    11101 cgcatccggc attggtgcac aattgccgga tgcggcgtga acgccttatc cggcctacgg
    11161 ttgagtgcag ttttgtaggc atgataagac gcgccagcgt cgcatcaggc atccggcacc
    11221 agcgcacaat tatgaaatga tacgctcccc ggcactaaaa tccgttatgt ctcccgccca
    11281 atcaatggga tataaccctc gcgccacatc gcgatggaac gttgagaatg gccaatcact
    11341 cacttgcttt acccagccat gttttactgg atttatataa atataatcaa catgatggcg
    11401 atagtctttg gtattgcgga tggtgtgctc ctaaaaacgt ggttgccaga tatttttcaa
    11461 tccacaagca tgggtgaact gctttttaat ctcccgccag cgggaggaaa aatcatcatc
    11521 gctttcgggt aatgtccata tgcaatgcat atgctccggc aaaacgaccc aggcgttaat
    11581 ttcaaaaggt ctgtctcgct taacgttaat aatggcgtta cggagcatct ggaactgagc
    11641 ggtcagaagg tggcttcgac gatttcgtag gttaaccgtg aaaaaccagg ttcccccttt
    11701 gatgtaatac cgacgatatt cagacatggc aactcctttt tctcaacggt aagaagaaaa
    11761 ggaaaggtcg gcaatgaggt gatggaataa cgggaaagcg gctcgcaaaa acgcacagat
    11821 ttgtaggcat gataagacgc gtcagcgtcg tatcaggcat ctgcgcacaa ctgccggatg
    11881 cggcgtgaat gccttatccg tcctacaaaa tcgtctcttc cgtcaaaatc cggcgcgcgc
    11941 ctaaaaagtg atcctgccag aaaggttcag ctaaccgact tacccgaatg gtttccccgg
    12001 tgcgcggtga ctcaataaac tgaccgtcgc ccagatacac ccccatatgg tcggcaatct
    12061 cgcggctgtg gatatggaaa aacagcaaat ctccccggcg caggtcgtta ttcgccacaa
    12121 tcgttgcccg atggtagtga tacatctcat tagccgtacg cgggagcttc gcctcgagga
    12181 tcttgttgta ggcataaaaa accaacccgc tacagtcaaa gcctttatca ggccgcgtgc
    12241 cgccccagac gtaaggcttg ccaagctgct gctccaggcg gtgaatcgcc acttcggtaa
    12301 tgttatgcag atggtcgctg cttaaaaagt ggttgttttc cgccagcgcc tgccagcgtc
    12361 tgtcactcgc cttgagcggt gcaggaaacc attctggatg ctgttttatc tgttcgcggt
    12421 tgtgctggcg tagcgccctt ctgctttcgg cattgccttc cacaatatag ctgacctgct
    12481 ttttcagatg ggtttggtac tgttttaata aacgcgcacg gttttgcatc cgctggcggg
    12541 cggcgtagct gaaagagata tgtgatgtgg tggcgctggc aaatgaagaa aacggcaaac
    12601 aaatcaacag taatcccgac aatacaaagg atggaatcga cggcaaggac atactcaata
    12661 atggctggtc atgcattgat gttacagtgt atcccggccc gtacctgaac aaacttaata
    12721 aaccattatt ataaaattaa taaatatatt tcagttaatt atgtaaaaac atccgtttat
    12781 taaattactc atagattgtc gctgttgctc atttgagcta caattcaagc tgaataaata
    12841 tacagcatag gagatatccc aatggctgct aacgcatttg ttcgcgcccg aatcgatgaa
    12901 gatctgaaga atcaggcggc ggacgtactg gccgggatgg ggctgaccat ctctgacctg
    12961 gttcgcataa ccctcacaaa ggtcgcgcgt gaaaaggcat tgccgtttga tttacgcgag
    13021 cctaatcaat taaccattca atcaatcaaa aacagcgaag ctggcgttga tgttcataag
    13081 gccaaagacg ccgatgattt atttgataaa ttaggagttt aaatatgatt caaagggata
    13141 ttgaactcgg gacaattttc aaaggatgta aaacttgcac aaaagcgtca taaggatatg
    13201 aataaattga aatatcttat gacgcttctt atcaataata ctttaccgct tccagctgtt
    13261 tataaagacc acccgctgca aggttcatgg aaaggttatc gcgatgctca tgtcgaaccg
    13321 gactggatcc tgatttacaa acttaccgat aaacttttac gatttgagag aactggaact
    13381 cacgcggcgc tctttgggta aagcccgctg ccgatgaaat ttgtaaaccc tgcgccgaca
    13441 ggcttgcgta aaactctttt ggtgataatg tgagccttcc caatccagac tattccgatt
    13501 tgggatccat tttgccgtcc tggcgtgtgt aaccgtttta tcaaggaata agcagtatgc
    13561 gtaaaatcgc attaattctt gcgatgttgt tgatcccgtg cgtttcgttt gccggtttgc
    13621 tgggcagcag tagctcgacc acgccagtga gcaaagagta taagcagcag ttgatgggat
    13681 cccctgtcta catccagatc ttcaaggaag aacgtacgct cgatctctac gtcaaaatgg
    13741 gcgagcaata tcaactgctc gacagctata aaatctgtaa atattccggc ggcttagggc
    13801 caaaacagcg tcagggcgat ttcaaaagcc cggaagggtt ttatagcgtc cagcgtaatc
    13861 agttaaaacc agacagccgt tactacaaag cgattaatat tggtttcccc aatgcctatg
    13921 accgtgcgca tggttatgaa gggaaatacc tgatgatcca cggcgattgt gtttccatcg
    13981 gctgctacgc gatgaccaat cagggcattg acgagatttt ccagttcgtt actggcgcac
    14041 tggtatttgg tcagccgagc gtgcaagtga gcatttaccc gttccgcatg accgacgcca
    14101 atatgaagcg ccataaatat tccaacttta aggacttctg ggagcaactg aagccaggct
    14161 acgactactt tgagcaaacc cgtaagccac cgaccgtttc tgtggtcaat ggccgttacg
    14221 tggtcagcaa gccgttaagc cacgaagttg tgcaaccaca actggcatca aactacacgc
    14281 tccccgaggc aaaataagcg ccattcgcct ggcataatct tttgccaggt ttcattgccc
    14341 gtcagcggct gtgtcgcaat caccgtgacc acatcattcg gtgtggtctg cgagctgaag
    14401 tcgatttcca catcctgatc cagcaacgtt gccacgccaa acggcgcgcg gcgggtgatc
    14461 cagtgcagat tggtcgagca atacgccatt acatagcgcc catccgaaag cagcatgttg
    14521 aaaacgccct tctgccgcag ttcatccgcc agtgaggcga tatatttaaa taccgccgcc
    14581 atattgcccg gcgtacgcgg ataacgctgc gttaatttat acagcagcca gcaaaaggct
    14641 ttttcgctgt cggttttacc taccgggcgg aagttgccgg tttccagtga tttatagccc
    14701 gtcagttgtc cgttatgggc ataagtccag ttacgtcccc ataactcgcg ggtaaacggg
    14761 tgagtatttt ccaacgccac ctcgccccga tttgcctggc gaatatgagc cacaaccgaa
    14821 caggatttta tcgggtagtc ctgaacaagt ttggcaatgg gggaattaaa gctgggttgt
    14881 ggatctttaa atgtgcgaca gcctttacct tcgtaaaagg taatgcccca gccatcttta
    14941 tgtggcccgg ttccaccacc acgctgcacc agcccggtga aactaaagca aatatcggta
    15001 gggacgttgg cgctcatccc gagcaattcg cacatcggta acccccacaa cagcgggagc
    15061 gcatggcccc cggcgaagat tgacttaaga attttcaggc cggatgcggc gtaaacgcat
    15121 tattcggcct acgccagact tacttaacca tctctttttc aattaactgg atcaggatat
    15181 ggatcacttt aatgtgaatt tcctgaatgc ggtcggcata accaaaatgt ggaacgcgaa
    15241 tttcaatatc cgccgtacca gccattttgc cgccatcttt gccggtcagg gtaatcactt
    15301 tcattccctt ctcacgcgcc gccgcgatcg ctttgatcac gtttgcagag ttaccagacg
    15361 tggagatccc cagcagtaca tcgccttcgc ggcctaccgc ttcaacatag cgggagaaaa
    15421 tatcattgaa accaaaatcg ttaccgacgc aggaaatatg gctgacgtca gagatggcaa
    15481 tcgccgggta gcccggacgg ttttcacggt agcgaccggt cagctcttcg gcaaagtgca
    15541 tggcgtcgca atgggagccg ccgttgccgc aggaaagcac cttgccaccg gctttaaagc
    15601 tgtctgctaa caggaccgcc gctcgctgaa tggcgtgaat attggcgtca tcttttaaaa
    15661 agttagccag cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga
    15721 ggatatcctt cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc
    15781 gagaagtacg ggcaggtgct atgaccagga ctttttgccc tgaagtgtag ataaaaacag
    15841 caacaatgtg agcttcgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc
    15901 actacaacca tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg
    15961 atgattttga gtattctcgc tacagttgtc ctgctcggcg cgttgttcta tcaccgcgtg
    16021 agcttattta tcagcagtct gattttgctc gcctggacag ctgccctcgg cgtcgctggt
    16081 ctgtggtcgg cgtgggtact ggtgcctctg gccattatcc tcgtgccgtt taactttgca
    16141 cctatgcgta agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg
    16201 ccgatgtcgc gcactgagaa agaagcgatt gatgcgggta ccacctggtg ggaaggcgac
    16261 ttgttccagg gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc
    16321 gcagaagagc aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat
    16381 ttccagatca cccatgaact ggcggatctg ccgccggagt tgtgggcgta tctgaaagag
    16441 catcgcttct tcgcgatgat catcaaaaaa gagtacggcg gcctggagtt ttcagcttat
    16501 gcccagtctc gtgtgctgca aaaactctcc ggcgtcagcg ggatcctggc gattaccgtc
    16561 ggcgtgccga actcattagg ccccggcgag ctgctgcaac attacggcac tgacgagcag
    16621 aaagatcact atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgacc
    16681 agcccggaag cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc
    16741 gaatggcagg gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctacattacg
    16801 ctggcaccga ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc ggagaaatta
    16861 ctcggcggtg cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc
    16921 gtggaaattg gtcgtcgcca cttcccgctg aatgtgccgt tccagaacgg accgacgctc
    16981 ggtaaagatg tcttcgtacc gatcgactac attatcggcg ggccgaaaat ggccgggcaa
    17041 ggctggcgga tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac
    17101 tcaaccggcg gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt
    17161 cagttcaaaa tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc
    17221 gggaacgctt atgtaatgga cgctgcggca tcgctgatta cctacggcat tatgctcggc
    17281 gaaaaacctg ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag
    17341 tcgattattg atgcgatgga tattaccggc ggtaaaggca ttatgctcgg gcaaagcaac
    17401 ttcctggcgc gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt
    17461 ctgacccgca gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg
    17521 gaagagatgg aagcggcgaa gaacaatgac gtcaacgtgt tcgataaact gttgttcaaa
    17581 catatcggtc acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt
    17641 ttaaccagca gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc
    17701 ctgagcgcca acctcgccct gctttctgat gtctcgatgg cagtgctggg cggcagcctg
    17761 aaacgtcgtg agcgtatctc ggcacgtctg ggtgatattt taagccagct ttacctcgcc
    17821 tctgccgttc tgaagcgtta tgacgacgaa ggccgtaatg aagccgatct gccgctggtg
    17881 cactggggcg tacaggatgc gctgtatcag gcagaacagg cgatggacga tttactgcaa
    17941 aacttcccga accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt
    18001 cattatctgg caccgtctga caagctggat cataaagtgg cgaagatttt acaagtgccg
    18061 aacgccactc gctcacgtat tggtcgcggt cagtacctga cgccaagcga gcataacccg
    18121 gttggtttac tggaagaggc gttggtggat gtgattgccg ccgacccgat tcatcagcgg
    18181 atctgtaaag agctcggtaa aaatctgcca tttacccgtc tggatgaact ggcgcacaac
    18241 gcgctggcga aggggctgat tgataaagat gaagccgcta ttctggtgaa agcggaagaa
    18301 agccgtctgc gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg
    18361 gtaaagttgc cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa
    18421 aggctccgtt tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag
    18481 gctgccggtc aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac
    18541 attcaaccag gtcagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt
    18601 catctgatta gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg
    18661 tttgcacgcg ctcatcacct gatacgtctc atctcccaac gttacggttt gtgcaggggt
    18721 ataagtaccg cctttcatca cccaggcagg cagcttatgc ccttgcacca tctgattaaa
    18781 tgcagctttg gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat
    18841 tgcactggtg gcgatgacca gtgcggcgac tgtcgttatt gccttaaaca tcattcctcc
    18901 cgagcttatc ctgcccatgt tattaacctc ctaaagcctg gcgataacac gataatttgc
    18961 aaatcagata tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc
    19021 ctgataaggt gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt
    19081 ttgaagatta cgcttttgca gcaaccactg gtgtggatgg atggtcctgc caacctgcgt
    19141 cattttgatc gtcaactgga aggtattacc gggcgcgatg tgatcgttct accggagatg
    19201 tttaccagcg gctttgccat ggaagcggct gcttcgtcgc tagcacaaga tgacgtagtg
    19261 aactggatga cagccaaagc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta
    19321 caaacggagt ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat
    19381 ttttatgata agcgtcatct gttccgcatg gcagatgagc atctgcatta taaagcgggc
    19441 aatgcgcgag tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac
    19501 ttacgttttc cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc
    19561 aactggcctg ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag
    19621 aatcaggcgt atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat
    19681 cgcggtgaca gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat
    19741 caggcaacgc gcattgatgc ggagctgtcg atggtggcat tgcgggaata tagagaaaag
    19801 tttccggcat ggcgggatgc ggatgagttt aggttgcggt gagtgggggc tgtatttagg
    19861 gcggggaatt cagaaaggga tgtttagtgt gctgagcgag agttaaataa taaaaaactg
    19921 ggaagattta ataatccccc cgcagggcaa gagcatgaat taaatctgtg ttaaattttt
    19981 cattttgtca gttcgaaacc caagctctga gcaggatgca agcacttctt ctatataaca
    20041 tgtgtttaac gcaaccttct ttcttttcct cttcacccca cccttaatgt ctttgcaatc
    20101 tcttaataaa ttcagtgcca tcttctttat tccagatatt atttcggctg cgtttcctct
    20161 tcttatccgg ctggcatctt cattcatttt tacatctaac acccaatgaa gactgtgctc
    20221 gatcagccag tgcgctctga tagcatgtgc aaattcttta gcatccatat cctttgatga
    20281 aatataatat cggatgctta caccttctgc tgatttatct tctttcttct gcctgaatga
    20341 caatgctaca caaagctttt ttaatccctt ccattcgaat tcaaaatcac aaaattcagg
    20401 cgttacgtta ctgacaatat gcaaacgtgt ttcttttctt ccatgactta tctcctgcgt
    20461 a
//
LOCUS       DAZKIF010000090        20412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZKIF010000090 DAZKIF010000000
VERSION     DAZKIF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.090"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            160..1395
                     /gene="aslB"
                     /locus_tag="ACHOTW_003195"
     CDS             160..1395
                     /gene="aslB"
                     /locus_tag="ACHOTW_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9845248.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(1563..3218)
                     /gene="aslA"
                     /locus_tag="ACHOTW_003196"
     CDS             complement(1563..3218)
                     /gene="aslA"
                     /locus_tag="ACHOTW_003196"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFP9845249.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(3897..5093)
                     /gene="hemY"
                     /locus_tag="ACHOTW_003197"
     CDS             complement(3897..5093)
                     /gene="hemY"
                     /locus_tag="ACHOTW_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP9845250.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(5096..6289)
                     /gene="hemX"
                     /locus_tag="ACHOTW_003198"
     CDS             complement(5096..6289)
                     /gene="hemX"
                     /locus_tag="ACHOTW_003198"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP9845251.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQADTPAA
                     APQGE"
     gene            complement(6311..7051)
                     /gene="hemD"
                     /locus_tag="ACHOTW_003199"
     CDS             complement(6311..7051)
                     /gene="hemD"
                     /locus_tag="ACHOTW_003199"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP9845252.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(7048..7989)
                     /gene="hemC"
                     /locus_tag="ACHOTW_003200"
     CDS             complement(7048..7989)
                     /gene="hemC"
                     /locus_tag="ACHOTW_003200"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP9845253.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            8376..10922
                     /gene="cyaA"
                     /locus_tag="ACHOTW_003201"
     CDS             8376..10922
                     /gene="cyaA"
                     /locus_tag="ACHOTW_003201"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP9845254.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(10962..11282)
                     /gene="cyaY"
                     /locus_tag="ACHOTW_003202"
     CDS             complement(10962..11282)
                     /gene="cyaY"
                     /locus_tag="ACHOTW_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP9845255.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(11444..11586)
                     /locus_tag="ACHOTW_003203"
                     /pseudo
     CDS             complement(11444..11586)
                     /locus_tag="ACHOTW_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            11745..11948
                     /gene="yifL"
                     /locus_tag="ACHOTW_003204"
     CDS             11745..11948
                     /gene="yifL"
                     /locus_tag="ACHOTW_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9845256.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11985..12809
                     /gene="dapF"
                     /locus_tag="ACHOTW_003205"
     CDS             11985..12809
                     /gene="dapF"
                     /locus_tag="ACHOTW_003205"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9845257.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12806..13513
                     /gene="yigA"
                     /locus_tag="ACHOTW_003206"
     CDS             12806..13513
                     /gene="yigA"
                     /locus_tag="ACHOTW_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFP9845258.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13510..14406
                     /gene="xerC"
                     /locus_tag="ACHOTW_003207"
     CDS             13510..14406
                     /gene="xerC"
                     /locus_tag="ACHOTW_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9845259.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14406..15122
                     /gene="yigB"
                     /locus_tag="ACHOTW_003208"
     CDS             14406..15122
                     /gene="yigB"
                     /locus_tag="ACHOTW_003208"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP9845260.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15206..17368
                     /gene="uvrD"
                     /locus_tag="ACHOTW_003209"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15206..17368
                     /gene="uvrD"
                     /locus_tag="ACHOTW_003209"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP9845261.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(17413..18315)
                     /locus_tag="ACHOTW_003210"
     CDS             complement(17413..18315)
                     /locus_tag="ACHOTW_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HFP9845262.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(18398..19162)
                     /gene="yigE"
                     /locus_tag="ACHOTW_003211"
     CDS             complement(18398..19162)
                     /gene="yigE"
                     /locus_tag="ACHOTW_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP9845263.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            19324..19380
                     /gene="ysgD"
                     /locus_tag="ACHOTW_003212"
     CDS             19324..19380
                     /gene="ysgD"
                     /locus_tag="ACHOTW_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP9845264.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            19532..>20412
                     /gene="corA"
                     /locus_tag="ACHOTW_003213"
     CDS             19532..>20412
                     /gene="corA"
                     /locus_tag="ACHOTW_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP9845265.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPG"
BASE COUNT         4673 a         5298 c         5442 g         4999 t
ORIGIN      
        1 ctctcgccga ccaattttga accccgcttc ggcggggttt tttgttttct gtgcatttcg
       61 tcaccctccc ttcgcaataa acgcccgtaa taactcattg ccccacggta tgatttcgcc
      121 cttaacgtat tgaaggatga cttcaggcaa ggagcgacca tgctgcaaca ggttccaacg
      181 cgtgcttttc atgtgatggc gaaaccgagc ggttccgatt gtaatctgaa ctgtgactac
      241 tgtttttatc tcgaaaaaca atccctttac cgcgaaaagc cagtcacgca tatggacgat
      301 gacacgctgg aagcgtatgt ccgtcactat atcgctgcca gcgaaccgca aaacgaagtg
      361 gcttttacct ggcagggcgg cgaaccaacg ctactcgggc tggagtttta ccgccgtgcc
      421 gtggcgctac aggcgaaata tggtgctggc aggaagataa gtaacagctt ccagactaac
      481 ggcgtactgc tcgatgatga atggtgtgca tttctggcag aaaatcattt tcttgttggg
      541 ttatcgctgg atggtccggc tgagatccac aatcaatatc gcgtgacaaa aggcggcaga
      601 cccacgcata aactggtgat gcgtgccctg acgctcctgc aaaaacatca tgtcgactat
      661 aacgtgctgg tctgcgtcaa ccgcaccagc gcgcagcaac cgttacaggt ttatgatttt
      721 ttgtgcgatg cgggagtcga attcatccag tttattccgg tggtcgagcg cctggctgat
      781 gaaacgactg cccgcgaagg actgaaacta catgcgcctg gtgatattca gggggaactg
      841 acggaatggt ctgtgcgccc cgatgaattt ggtgaatttc tggtggcgat attcgaccac
      901 tggattaaac gcgacgtcgg caagattttc gtgatgaata tcgaatgggc gtttgccaat
      961 tttgtcggtg cgccgggtgc ggtttgccat catcagccaa cctgtgggcg ctcggtgatt
     1021 gttgagcaca atggcgacgt ttacgcctgc gatcactatg tttatccgca atatcgactg
     1081 gggaatatgc accagcaaac aattgcagaa atgatcgatt ccccgcaaca gcaggtgttt
     1141 ggtgaagata aatttaagca attaccggcg cagtgtcgca gttgtaacgt gttaaaagca
     1201 tgttggggag gctgcccgaa acaccgcttc atgctcgatg ccagcggcaa accgggactg
     1261 aattatttgt gtgccgggta tcagcgttat ttccgccatc taccgccata tcttaaagca
     1321 atggctgatt tgctggcgca cggtcgcccg gccagcgaca ttatgcaggc gcatttgctg
     1381 gtggtgagta agtaaaaaag cggccatttt gtggtcgcct gcggttgatt gccggatgcg
     1441 gcgtaaacgc cttatccggc ctacatgatc gtgcaaattc aataaattgc agcgttctgt
     1501 aggctggata agatgcgtca gcatcgcatc cggcaaaggc agatctcagc gatagcgccg
     1561 gcttaatcag atttaatctg cgcgcgtggt ggatactttt tcaggatctc catatacgcg
     1621 tgcatttcgg tctgtagcgg tacacccatc ggaatatggc gcacgccgat agagtcgctc
     1681 tcctgcggat cggtgtagag gttaaacacc gacgatccgg ctgtttgcat tactgtgccg
     1741 gtgaatccac cctgatatcc gctctgggta taagcgtaag gttgctgaat caggacgtga
     1801 tacttgaatt catccatacg cacagcagcg agtttaccgt tgaggaagta gtgctcggcc
     1861 ttacggttag actgaccatt tgttcccagg aagaaagatg tctggtccac accatcgata
     1921 aaggtggttt tcggcactaa attcgccact ttcgcaccag gatgccccgc cagatccagt
     1981 gctgttggga agagatctgc cagatcaaca ataccgtcag atttacgcgg ttggatcatg
     2041 cctttccagt aaacgaaagt cggtacgcga acgccgcctt cccaggtaga tcctttcgca
     2101 ccacggaacg gtgtgcgtcc gtgcggcggt acttcggctt ccggtccgtt atcggaggta
     2161 aagacaatca gcgtgttatc aagctgaccg tttttctcca gtgctttata cagattagcg
     2221 aacacatcgt tcatttccac catgcagtcg ccatacgagg tgcgtgccgg agagctaccc
     2281 gcatatttgg cgttcgggta gttatcgaag tgacagccgc gagtgccgta atagaggaag
     2341 aaaggcttat cactcttcgc catcttgtcg aggaacttaa cgccatattc catccagcgt
     2401 tgatccagat cttccatata tttcggcgta atgtcggcaa tggcctgttg ttcgccgccg
     2461 cgcaccgcat gaacgtcatc tttgctaaat ggtaattgct tgatgtattc agaacggtcc
     2521 ggactcaggg ccacttccgg attgacgtga acgtcgcgcc attcggtgta catatcagac
     2581 accgagttaa agccacggaa atcatcaaag ccaacgttct gcggctgcga ctctttgttt
     2641 tcccccatat gccatttccc gatggcctga gtgacgtagc cctgatcgtg cagcaactgc
     2701 ggcagcgtgg ttaacccttg cagcccgccc ggttgcccgt acattggcgg catcagaatg
     2761 ccgtggtgga tggagtattg tccggtgaga atggtggcgc gggttgggga agagcttggt
     2821 tgagaatacg ccgaagttaa aatcagcccc tggctggcaa cggcgtcgat atctggtgta
     2881 gggttaccca ccgccacgcc gccaccgtta aaaccgacgt ccatccagcc cacatcgtcc
     2941 agcaagaaaa caaccacatt cggtttctta ccggtttttt tctcaagttc tgccagcttc
     3001 tgctggggtt ctttatcctg cgccggatgc tgcattactg gcatcatgtt gtcggcaata
     3061 gtggtcgccg gtttaaccag atactggttt gggtgatcgt atccggcaaa gcctttgcgc
     3121 gcggtggcag ttgacggggt atctgctgcg ctggccatga gaggaagagc ggcggcgaca
     3181 gcaacaacaa gaagtttggg tgaaaacgaa aattccatgc aaaatgctcc ggtttcatgt
     3241 caacaaaatg atgacgtaat taagcattga taattgagat ccctctccct gacaggatga
     3301 ttacataaat aatagtgata aaaataaatt atttatttat ccaagaaatg aatttaaaaa
     3361 tcaggagagc gttttcaatc ctacctctgg cgcagttgat atgtaaggca ggtttattat
     3421 atcgcgttga ttattgatgc tgtttttagt tttaacggca attaatatat gtgttattaa
     3481 ttgaatgaat tttatcattc ataataagta tgtgtaggat caagctcagg ttaaatattc
     3541 actcaggaag ttattactca ggaagcaaag aggattacag aattatctca taacaagtgt
     3601 taagggatgt tatttcccga ttctctgtgg cataataaac gagtagatgc tcattccatc
     3661 tcttatgttc gccttagtgc ctcataaact ccggaatgac gcagagccgt ttacggtgct
     3721 tatcgtccac tgacagatgt cgcttatgcc tcatcagaca ccatggacac aacgttgagt
     3781 gaagcaccca cttgttgtca tacagacctg ttttaacgcc tgctccgtaa taagagcagg
     3841 cgttttttta tgtgtcagga aggtcccgga ggtgcttgcc tccgggtgag aaggaactac
     3901 tgtggtgggt tgttctgcaa cgttaacatc aaaccgtcgc gacgcatcgc tgcagcttct
     3961 tccggcttgt gcagtctgtc cagcgcgtcg gcaagccatg cgtaatcgta ggcgtccgga
     4021 cgttgtttca gcgctgcgcg gaaggcgagc gatgcttcct gccattctcc gtgcttcatc
     4081 agtgactggc ccagtgtgct ccacaacagc gggcgatcgc cgacgttttt gatttgctgg
     4141 cgcagcactt tttccagctg ttccggattg tttgttttca gtcgaggaat cggcagcagt
     4201 aggcgatcgt cgtactggcg tttcaggcca tcgatgataa tttgctgggc agtatcatga
     4261 tcgtcacatt caataagatg ttccgccatt gccacctgca acgctacctg atgacgcgtt
     4321 ttccggcttt ggtttttcca ccagttacgc aaaccttcgc taccgttatc ggcacgcgcc
     4381 tgatccatca ggccaatcca tgcctgttgt tccagcattg cacgatgttc ttcatcacca
     4441 acatgggctt tcgccattga tgggataata tccagcagcg aactccatgc acctgtgcgg
     4501 atatacgcct gttccgccag acgtaatact tccggatggc gtggcgtaac ttccagcagc
     4561 ttatccacgc cgtggcgtgc agcatggttt tcattacggg ccagttgcag acgtacacgg
     4621 gtgatttcta ccggaatggt gtcattgccg gccagctccg ctgcgcgttc cagatgttgg
     4681 ttggcgcgcg cttcatcacc acgttgttgc gcggcttcgg cagccagtag atagttcacc
     4741 accggttgtt cagcgtgatc ggcatttttc gccatcagct tttcaacttg ctgataatcg
     4801 ccttccgcca gtttcagcag cgcctgttcg gtctgcttac gtgcacggcg acgcttacgc
     4861 ccgacaaacc acccacgggt gtgcgcgcca gtgcggaaga tccgccgcag tagccactca
     4921 atggcaaaca gcactaccat cgccagaatc aatatgatcg ccaggcccgt gacgctggtt
     4981 tcgatattgt agttgtcggt ctggatcagc acataaccct gatgaccggc aatcatcggg
     5041 ccaaccacga tccccgcaat cagcaacaca aagagcaata acacttttag catggttatt
     5101 ctccttgcgg cgcggctgcc ggagtatcag cttgcggtgc aggtgcaggt gcaggtgcag
     5161 gtttagcttc cgttgtcccc gctgccggtt gtgccagcag gttacgcacg cgagtctgca
     5221 tcagtttttc cagcatcgcc tggctttgca gggtttccgg aagatccatc gagatatttt
     5281 gctggcttaa ctggtctacc tcgtcgagga acgctttggt ggtggcatca tcagtatcgt
     5341 agtaagcacg tacccaggtg gagacgttct ccagcgcctg gcgataagtc tcttgctggt
     5401 gacgcggtac agcttgtgct gcgaccagca ggcgagagcg aatattttcg cgcagataga
     5461 tatcctgatt tggcgctaac agcggtacgg cggtgtcatc acgacggcga atcgtaatga
     5521 agttgtccat aaagttctgc cagctttttt gcagattgat acgccattcg ctgatggaac
     5581 tggaaagctc ttcaccgtcg gaatccatcg gcgaaccatc gctgtcatta tcggccagac
     5641 gcaggttatc tacctgattt gaaagctgat taagcttaag gatgatgccg tcataatcca
     5701 cctgcgatac tgcagaaagg ctggcgatat catcggtaat tgcccgacga acggtaatca
     5761 gactcgggtc attcatatcc gccaggctgg cgtctgcact tttcagcaac gctgcagcgg
     5821 tcgtgacgtc ctgatcgctc cacagcttcc gtccggcgag tttcaccaga aaatcggcct
     5881 gagccagcag ccaggtttta gcatcgctgc cggaaatggt ggcgaccttt tgttggactt
     5941 catccaactg ttttgccagc gtttcttgct gacgattcgc ctgcttaagc tgtgcagctt
     6001 gttgcttaat aatgccttcc agctcgactt tttggctctc ctgggctttt tgcaatgccg
     6061 tcagttggtt agccagggca tcgctggtgg cggtctgatt gacggcctgt tgtttacccc
     6121 agccatacaa accgacgccc gccgccagag caatagcgat agccaccgcg ctgagaatca
     6181 atgcggtatt gttcttactc tttttttctg ttgcgacagg ttgtgacgtg gtgtccacgg
     6241 cctccctggt ctcttcaacc acggcggagg ttttttcttg ttccgtcatt atggcttcct
     6301 gttatgagag ttattgtaat gcccgtaaaa gcgcatcgtt gtcagcgtta tcggcgacct
     6361 taatgtcttg ccagcccagt tcccgggcga gtttcgccaa acgctcactg acgaccaata
     6421 gtcgacagtg tagtaaccag tgctcacgat accattgtgg gatcagcgac cagagttgct
     6481 gcaacatttc accgctggta acaacgaccg tcgtcacctc gcgggattgc cagcgcatcg
     6541 cttcttctgc accatcgtaa tggattgcgc atcgttgata acattcacaa aaagtgacct
     6601 cagcaccgcg cgccgtcagg gtatccccaa ttagctcacg accgccattg ccacgtaata
     6661 tcagcgcacg tttgcccgca atattttgta attcaggtaa ttgtagcaag acttcgctga
     6721 tttcccgatc ctgcgggtag agaatcttct gtccgcttac ggtatgtagt gccagtgcgg
     6781 tggtgcgtcc aatggcgaaa taattaggta gtcggggcca tttacgatct tgctgatgta
     6841 gctgtgattg ggcaaaagca accgcgtgtt gcgagagggc aaacaacaga tcgctctccc
     6901 ccagcgctgc cagttgatca gcaagttgcg gtaattgtcg acccggagaa aactcaatca
     6961 gcggaaaatg ccaggccacc tgccccagtg tgcgcagacg gctcactaac tcttctccag
     7021 cgggagacgg gcgggtgaca aggatactca tgccggggcg tctccgttat agacttcagc
     7081 gaggatctcg cgcgcgccgt tattcagtag ctcttctgcc agcgaaatcc ccatttgttc
     7141 ggcatcttgc ggcgcaccgc ggcgttcacc gcgaataatc tgcgaaccgt ccggcgcgcc
     7201 gaccagcgca cgcagccaga tttcgccatc aataagctcg gcgtagctac caattggcac
     7261 ctgacatcca ccttcgagac gggtattcat ggcgcgttct gcggtaacgc gcagtgcagt
     7321 ttcgtggtga ttcagcgcgg caagcagctc gcgagtgcgt gtatcatcaa ggcggcattc
     7381 aataccgact gcaccttgtc ctaccgccgg aagagaaatc tcgggtggca acgcggcgcg
     7441 aatgcgggac tccagaccta aacgttttag tccggctacg gcaagaatga tggcatcgta
     7501 ttcgccgtta tccagtttgc tcaggcgagt gccgacgttg ccgcgcaggg agcggataat
     7561 cagatccggg cggcgttcag ccagttggca ctggcgacgt aaactggacg tcccgacgat
     7621 actgcctgcc ggtaacgcat ccagactgtc atagttattg gacacaaagg catcgcgagg
     7681 atcttcacgc tcacaaatag tgaccaggcc cagaccttgc gggaattcaa ccggcacatc
     7741 tttcattgaa tgtacggcga tatcggcgcg attttcgagg agcgcgactt ccagctcttt
     7801 tacaaataag ccttttccgc cgactttcgc cagcggcgta tcaagaatca catcgccgcg
     7861 cgtcaccatc ggtaccagtt caacgaccag gcccggatgg ctcgccatca acttgtcttt
     7921 gacatagtgt gcctgccaga gtgcaagtgg gctttggcgt gtggcaattc ttaaaacatt
     7981 gtctaacatg cttgttaccg tcattatcat ccgtggtcca tcctaacatc cttgccagag
     8041 tgatgtcagt gttgtggtga aacgtagacg cctgcgcaaa ccgtaaaatg aggtctggca
     8101 gtggatcctg acaggcgttt cacgccgtcg taataaggaa tttacagaga ataaacggtg
     8161 ctacacttgt atgtagcgca tctttcttta cggtcaatca gcaaggtgtt aaattgatca
     8221 cgttttagac cattttttcg tcgtgaaact aaaaaaacca ggcgcgaaaa gtggtaacgg
     8281 ttacctttga catacgaaat atcccgaatg ccgcgtgtta ccgttgatgt tggcggaatc
     8341 acagtcatga cgggtagcaa atcaggcgat acgtcttgta cctctatatt gagactctga
     8401 aacagagact ggatgccata aatcaattgc gtgtggatcg cgcgcttgct gctatggggc
     8461 ctgcattcca acaggtctac agtctactgc cgacattgtt gcactatcac catccgctaa
     8521 tgccgggtta ccttgatggt aacgttccca aaggcatttg cctttacacg cctgatgaaa
     8581 ctcaacgcca ctacctgaac gagcttgaac tgtatcgtgg aatgtcagta caggacccgc
     8641 cgaaaggtga gcttccaatt actggtgtat acaccatggg cagcacctcg tccgtagggc
     8701 aaagttgttc ctctgacctg gatatctggg tctgtcatca atcctggctc gatagcgaag
     8761 agcgccagtt gctacaacgt aaatgcagcc tgctggaaag ctgggccgcc tcgctgggtg
     8821 tggaagttag cttcttcctg attgatgaaa accgttttcg ccacaacgag agtggcagtc
     8881 tggggggcga agattgtggc tccacccagc atatactgct gcttgatgaa ttttatcgta
     8941 ccgccgtgcg tctcgccggt aagcgtattc tgtggaatat ggtgccgtgc gacgaagaag
     9001 agcattacga tgactacgta atgacgctct acgcacaagg cgtgctgacg ccaaatgaat
     9061 ggctggatct cggcggctta agctcgcttt ccgctgaaga atacttcggt gccagccttt
     9121 ggcaactcta caagagtatc gattctccat acaaagcggt gctgaagaca ctgctgctgg
     9181 aagcctattc ctgggaatac ccgaacccac gcctgctggc gaaagatatc aaacagcgtt
     9241 tgcacgacgg cgagattgta tcgtttggcc tcgatcctta ctgcatgatg ctggagcgtg
     9301 ttactgaata cctgacggcg attgaagatt tcacccgtct ggatttagta cgtcgctgct
     9361 tctatttaaa agtgtgcgaa aagctcagcc gtgaacgcgc ctgcgtaggc tggcgtcgcg
     9421 cagtgttgag ccagttagtg agcgagtggg ggtgggacga agctcgtctg gcaatgctcg
     9481 ataaccgcgc taactggaag attgatcagg tgcgtgaggc gcacaacgag ttgctcgacg
     9541 cgatgatgca gagctaccgt aatctgatcc gctttgcgcg tcgcaataac cttagcgtct
     9601 ccgccagtcc gcaggatatc ggcgtgctga cgcgtaagct gtatgccgcg tttgaagcat
     9661 taccaggtaa agtgacgctg gtaaacccgc agatttcacc cgatctctcg gaaccgaatc
     9721 tgacctttat ttatgtgccg ccgggccggg ctaaccgttc aggttggtat ctgtataacc
     9781 gcgcgccaaa tattgagtcg atcatcagcc atcagccgct ggaatataac cgttacctga
     9841 ataaactggt ggcgtgggca tggtttaacg gcctgctgac ctcgcgcacc cgcttgtata
     9901 ttaaaggtaa cggcattgtc gatttgccta agttgcagga gatggtcgcc gacgtgtcgc
     9961 accatttccc gttgcgctta cctgcaccga caccgaaggc gctctacagc ccgtgtgaga
    10021 tccgccatct ggcgattatc gttaacctgg aatatgaccc gacagctgcg ttccgcaatc
    10081 aggtggtgca ttttgatttc cgtaagctgg atgtcttcag ctttggcgag aatcaaaatt
    10141 gcctggtagg tagcgttgac ctgctgtatc gcaactcgtg gaatgaagtg cgtacgctgc
    10201 acttcaacgg cgagcaatcg atgatcgaag ccctgaaaac tattctcggc aaaatgcatc
    10261 aggacgccgc accgccagat agcgtggaag tcttctgtta tagccagcat ctgcgcggct
    10321 taattcgtac tcgcgtgcag caactggttt ctgagtgtat tgaattgcgt ctttccagca
    10381 cccgccagga aaccgggcgt ttcaaggcgc tgcgcgtttc tggtcaaacc tggggcttgt
    10441 tcttcgaacg cctgaatgta tcggtacaga aactggagaa cgccatcgag ttttatggcg
    10501 ctatttcgca taacaaactg cacggcctgt cagtgcaggt tgaaaccaat cacgtcaaat
    10561 taccggcggt ggtggacggc tttgccagcg aagggatcat ccagttcttt ttcgaagaaa
    10621 cgcaagatga gaatggcttt aatatctaca ttctcgacga aagcaatcgg gttgaggtgt
    10681 atcaccactg cgaaggcagc aaagaggagc tggtacgtga cgtcagtcgc ttctactcgt
    10741 catcgcatga ccgttttacc tacggctcaa gcttcatcaa cttcaacctg ccgcagttct
    10801 atcagattgt gaaggttgat ggtcgtgaac aggtgattcc gttccgcaca aaatctatcg
    10861 gtaacatgcc gcctgccaat caggatcacg atacgccgct attacagcag tatttttcgt
    10921 gatgaacgtg ccggaaagcg aggcttatcc ggcatgcaat cttagcggaa actgactgtt
    10981 tcacccgcct gctgcgtcgc cgcctgttcc agcaaatccc ggaaggtttc gccgctgcga
    11041 tcgcaaatcc actcatcgcc tttcagatca aaatggtagc cgccttgttt ggttgccagc
    11101 catacctggt gcagcggttc ctggcggttg ataatgattt tgctgccatt ctcaaaggta
    11161 atggtcagca cgccgccgtt gatttcgcag tcgatatcgc tgtcgccatc ccagtcgtcc
    11221 agacgctctt caatggtcag ccacagttga tcagccaggc gatgaaattc actgtcgttc
    11281 attgttgtat cctgttttta agtgatggcg gcagtatagc ggcatggggt cagggcttca
    11341 aagtttgcac ctctgcggct gcgttacggc atgattcatc cgtcaccgga ataatgatgt
    11401 ctctgtgtag cgaaagattt gtctcttcat tagggcgcag ttacaccacg cctttccctg
    11461 tttctggtaa acattatgat caggtttacc gagcgagcat cctcacgctg acggaactaa
    11521 aaaagccaac aaacaaacca cattgcgata gtgcataaag ccatcctggc gtgaggtgtt
    11581 gatcacgaaa ctgccagcag aacataaatc ctcacgagta agcgttatac tcgcagcatt
    11641 tcctcacttt tcagacttca taaagagtcg ctaaacgctt gcttttacgc cttctcctgc
    11701 gatgatagaa agcagaaagc gatgaacttt acaggcaatc cataatgaaa aacgtgttta
    11761 aggcactcac tgtattactt actctcttca gcctgacggg ctgcggtctg aaaggtccgc
    11821 tctatttccc gcctgcagat aaaaatgcac cgccgccgac caaaccggta gagacgcaaa
    11881 cgcaatccac ggtgccggat aaaaacgatc gcgccactgg cgatggtcca tcccaggtga
    11941 attactaaaa gtcagtttct gtacccgcgt gattggagta aatgatgcag ttctcgaaaa
    12001 tgcatggcct tggcaacgat tttatggtcg tcgacgcggt aacgcagaat gtcttttttt
    12061 caccggagct gattcgtcgc ctagctgatc ggcacctggg ggtagggttt gaccaactgc
    12121 tggtggttga gccgccgtat gatcctgaac tggattttca ctatcgcatt ttcaatgctg
    12181 atggcagtga agtggcgcag tgcggcaacg gcgcacgctg ctttgcccgt tttgtgcgtc
    12241 tgaaagggct gaccaataag cgcgatatcc gcgtcagcac cgccaacggg cggatggttc
    12301 ttaccgtcac cgatgatgat ctggtccgcg taaatatggg cgaacccaac ttcgaacctt
    12361 ctgccgtgcc gtttcgcgct aacaaagcgg aaaagaccta tattatgcgc gccgccgagc
    12421 agacaatctt atgcggcgtg gtgtcgatgg gaaatccaca ttgcgtgatt caggtcgatg
    12481 atgtcgatac tgcggcggta gaaacactgg ggcctgttct ggaaagccac gagcgttttc
    12541 cggagcgcgc caatatcggt tttatgcaag tggttaagcg cgagcatatt cgtttacgcg
    12601 tttatgagcg tggggcagga gaaacccagg cctgcggcag cggcgcatgt gcggcggttg
    12661 cagtagggat tcagcaaggt ttgctggccg aagaagtacg cgtggaactc ccaggcggtc
    12721 gtcttgatat cgcctggaaa ggtccgggtc acccgttata tatgactggc ccggcggtac
    12781 atgtctacga cggatttatt catctatgaa gcaaccaggg gaagaactgc aggaaacact
    12841 cacggagctt gatgaccggg tggttgtcga ttatctgatt aaaaatcctg agttttttat
    12901 ccgcaatgcg cgcgcagtag aagcgatacg tgtgccgcat ccggtacgcg gtaccgtttc
    12961 gttggtcgag tggcacatgg cccgcgcacg taatcatatt catgttctgg aagagaacat
    13021 ggcgctgttg atggaacagg ctatcgccaa cgaaggcctg ttttatcgct tactctacct
    13081 gcagcgcagt ctcaccgccg ccagcagtct tgatgatatg ctgatgcgct ttcaccgctg
    13141 ggcgcgtgat ctcggcctgg caggtgcgag tctgcgcctg tttccggatc gctggcgctt
    13201 aggtgcgccg tcgaaccaca ctcatctggc attaagccgt cagtctttcg aaccgctgcg
    13261 tattcagcgt ctggggcagg agcagcatta tctggggccg cttaacggac cggagctgct
    13321 ggtggtacta ccggaagcga aagcggtggg atcggtggcg atgtcgatgc tgggaagcga
    13381 tgctgattta ggcgtggtgc tgttcaccag ccgcgatgcc agtcactatc aacaagggca
    13441 gggaacgcag ttacttcatg aaattgcgct gatgttgccg gagcttctgg agcgttggat
    13501 tgaacgcgta tgaccgattt acacaccgat gtagaacgct acctacgtta tctgagcgtg
    13561 gagcggcagc ttagtccgat aactctgctt aactaccagc gacagcttga ggcgatcatc
    13621 aattttgcca gcgaaaacgg cctgcaaaac tggcagcaat gcgatgcagc gatggtgcgc
    13681 aattttgctg tacgcagtcg ccgtaaaggg ctgggcgcgg caagcctcgc attacgcctc
    13741 tctgcgctac gtagcttttt tgactggctg gtcagccaga acgaactcaa agctaacccg
    13801 gcgaaaggtg tttcggcacc gaaaacgcca cgtcatctgc cgaaaaatat cgacgtcgat
    13861 gatataaatc gcctgttgga tattgatatc aacgatcccc tcgctgtgcg cgaccgtgca
    13921 atgctggaag tgatgtacgg cgcgggcctg cgtctttccg agctggtggg gctggacatc
    13981 aaacacctcg acctggagtc cggcgaagtg tgggtgatgg ggaaaggcag caaagagcgc
    14041 cgcctgccga ttggtcgtaa cgctttgtca tggattgagc actggcttga tttgcgcgac
    14101 ctgtttggta gcgaagacga cgcgcttttt ctgtcgaaac tgggcaagcg tatctccgcg
    14161 cgtaatgtgc agaaacgctt tgccgaatgg ggcataaaac aagggctgaa taatcacgtt
    14221 cacccgcata aattacgtca ctcgttcgct acgcatatgc tggagtcgag cggcgatctt
    14281 cgtggtgtgc aggagctgct gggtcatgcc aacctctcca ccacgcaaat ctatactcat
    14341 cttgattttc aacaccttgc ctcggtgtac gatgcggcgc atccacgcgc caaacggggg
    14401 aaataatgcg tttttaccgg cctttggggc gcatctcggc gctcaccttt gacctggatg
    14461 atacccttta cgataaccgt ccggtgattt tgcgcaccga gcaggaggcg cttacctttg
    14521 tgcaaaatta ccatccggcg ctgcgcagct tccagaatga agatctgcaa cgcctgcgcc
    14581 aggcggtacg ggaagcggaa cccgagattt atcacgacgt gacgcgctgg cgttttcgct
    14641 caatagaaca agcgatgctt gacgccggat tgagcgcgga agaagccagt gcaggcgcac
    14701 acgcggcaat gatcaacttt gctaaatggc gcagcaggat cgacgtcccg cagcaaaccc
    14761 acgacacctt aaaacagctg gcgaagaaat ggccgctggt ggcgatcacc aacggtaacg
    14821 ctcagccgga actgtttggt ttgggggatt attttgagtt tgtgctgcgc gctggcccgc
    14881 acgggcgctc gaaaccgttc agcgatatgt acttcctggc ggcggaaaaa ctcaacgtgc
    14941 cgatcggcga gatcttacat gtaggtgacg atctcaccac tgacgtgggt ggggcaattc
    15001 gcagcggaat gcaggcctgt tggatcagac cggaaaatgg cgatctgatg caaacctggg
    15061 atagccgttt actcccgcat ctggaaattt cccggttggc atctctgacc tcgctgatat
    15121 aatcagcaaa tctgtatata tacccagctt tttggcggag ggcgttgcgc ttctccgccc
    15181 aacctatttt tacgcggcgg tgccaatgga cgtttcttac ctgctcgaca gccttaatga
    15241 caaacagcgc gaagcggtgg ccgcgccgcg cagcaacctt ctggtgctgg cgggcgcggg
    15301 cagtggtaag acgcgcgtac tggtgcatcg tatcgcctgg ctgatgagcg tggaaaactg
    15361 ctcgccatac tcgattatgg ctgtgacgtt taccaacaaa gcggcggcgg agatgcgtca
    15421 tcgtatcggg caactgatgg gcaccagcca gggcggcatg tgggtcggca ccttccacgg
    15481 gctggcgcac cgtttgctgc gtgcgcacca tatggacgcc aatctgccgc aggatttcca
    15541 gatcctcgat agcgaagacc agctacgcct gcttaagcgt ctgatcaaag ccatgaacct
    15601 cgacgagaag cagtggccgc cgcgccaggc aatgtggtac atcaacagcc agaaagatga
    15661 aggcctgcgt ccgcatcata ttcaaagcta cggtaatccg gtggagcaga cctggcagaa
    15721 ggtgtatcag gcgtatcagg aagcgtgcga tcgtgcaggc ctggtggact tcgccgagct
    15781 gctgctgcgc gctcacgagt tgtggcttaa caagccgcat atcctgcaac actaccgcga
    15841 acgttttacc aacatcctgg tggacgaatt ccaggatacc aacaacattc agtacgcgtg
    15901 gatccgcctg ctggcgggcg acaccggcaa agtgatgatc gtcggtgatg acgaccagtc
    15961 aatctacggc tggcgcgggg cgcaggtgga gaatattcag cgtttcctca atgatttccc
    16021 cggtgccgaa actatccgtc tggagcagaa ctatcgttct accagtaaca ttctgagcgc
    16081 cgcgaacgcc ctgattgaaa acaacaacgg gcgtctgggt aaaaaactgt ggaccgatgg
    16141 cgcggacggt gagcctattt ccctctattg cgcttttaac gaactcgatg aagcgcgttt
    16201 tgtcgttaac cgcatcaaaa cctggcagga caacggtggg gcgctcgccg agtgcgccat
    16261 tctctaccgc agcaacgccc agtcgcgggt gctcgaagag gcgttattgc aggccagtat
    16321 gccgtaccgt atttacggcg ggatgcgctt cttcgaacgc caggaaatca aagatgcgct
    16381 ctcgtatctg cgcctgattg ccaaccgcaa cgacgacgcg gcctttgagc gtgtggtgaa
    16441 tacgccaacg cggggtattg gtgaccggac gctggacgtg gtacgtcaga catcgcgcga
    16501 tcgccagtta acactctggc aggcatgtcg tgaactgttg caggaaaaag ccctcgccgg
    16561 acgtgctgcc agcgccttac agcggtttat ggaactgatc gacgccttag cgcaggaaac
    16621 tgccgatatg ccgctgcatg tacagactga ccgggtaatt aaagactccg gcctgcgtac
    16681 catgtacgag caggagaagg gcgaaaaagg tcagacgcgt atcgaaaact tagaggaact
    16741 agtgacggca acgcgccaat tcagctacaa cgaagaagac gaagatttaa tgccgctgca
    16801 ggcgttcctc tcccatgcgg cgctggaagc gggcgagggg caggcggata cctggcagga
    16861 tgcagtgcag ttgatgacgc tgcactcggc gaaaggcctg gagttcccgc aggtgtttat
    16921 cgtcggtatg gaagagggca tgttcccaag ccagatgtcg ctggatgaag gcggacgtct
    16981 ggaagaagaa cgccgtctgg cctacgttgg tgtaacccgc gcgatgcaga aactgacgct
    17041 gacctacgcg gaaactcgcc gtctgtatgg caaagaggtt taccatcgcc cgtcgcgctt
    17101 tatcggtgag ttgccggaag agtgtgtgga agaggtgcgc ctgcgcgcca cggtaagccg
    17161 cccggtcagc catcagcgta tgggtacgcc gatggtcgag aacgacagcg gctacaagct
    17221 cggccagcgt gtacgccacg ctaagtttgg tgaaggcact atcgtcaata tggaaggcag
    17281 cggcgagcat agccgtttgc aggtggcatt ccagggccag ggtattaaat ggctggtggc
    17341 ggcatacgcc cggctggaga cggtataacg ttgcgggatg cggtgcgcca caccgcatcc
    17401 ggcatcagca ccttacttat cacatttctt cacatcaata ttccagtcag caccagacgc
    17461 aacctgatgt ttctgcgcga aattatccat attcagcgcc acggaaacat tcaataagct
    17521 gttcagatac accatcggct gtccttccgg cacatcgccg aagcttgcga catacggcat
    17581 tttccctgcg tatttttgcc gggaaccttc ggaaatggtc acacacaacg tgtcgttgag
    17641 tttgatcccc gcctgattca gcaactcgtc gctgatattg ctccagacgt tgccgtactg
    17701 aatatcgaga atcgggatat tgcccttcac ttcacctttt gttgcttttg ctttctgata
    17761 agaaagttcg acaacctttg caggaagctc aggccccacc tgctcgaacg tgatcgcccc
    17821 ggaagccaga cgtgcaccgg tgtaggcata cacgtcacga ccgtggaagg tatatgactt
    17881 ttcagaacct ttcaggcggt tagctttttc gtcgatttcc cggacgctct caatacccag
    17941 cgattccgcc accaaagtga gcgtaccgtt atccggtgaa acgaagtatt gaccgttttt
    18001 ggttttcagc actatcgatt tacggtcagt gcctacgccc ggatcgacca cactgacaaa
    18061 taccgaacct tgcggccagt aactggcggt ctgatacagg cggtaagcgc cttcccagat
    18121 gttatacggt ggaatttcgt gtgttaaatc aaatatttta agattatggt caacaccgaa
    18181 tgcgacccct ttcatcgccg agacggcacc atctttcagg ctaaaatcag tttgtaaaat
    18241 caaagcgtta tccgcataag cggatgatga tcccagacac gttaataaaa ataacgttgc
    18301 gagcgttttt ttcatttttg tttcccttca tttatctgat gattttccag ctgtgtagcc
    18361 agccccagtt tgtctggcct cctcgagtgt acaatgctta acctttccgc tcgacggaaa
    18421 tcatggtgac aaaaggatag cgttgccacg ggattgcccc gcctttcata tacatatgtg
    18481 aaatcgtgcc atcaagataa agcagctgct caacgttcag tttcgctttg gcataacagg
    18541 caaaatcata aaaatttgtc gcctgctggc tcaacaaaaa cacggcattc ccatgtttat
    18601 taattccaac accgttacga attttacgtg aggcgacgtt gggatgaata cgcggattaa
    18661 ttacactgtt ttccatcaac attggccctg actgcaccgc aaactgaatc tctttactgg
    18721 ttttgaaggc atccagacga acgatgccga ctttatctcc cgcgacataa aacacgccgc
    18781 caggacggat aaagaaattc ccttcacctg aagcgagatt taacgccacc ttctgctgac
    18841 cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg ccgttcatcg
    18901 ccatctgcac ctgaccctga ctattcatat ccgccagcag agcatgtaaa gttccccacg
    18961 cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga ttaacggtat
    19021 acgcctgtac ggtcagcgtt ggatctgaga gtgcgcaatc atcagcggca acagcaaaca
    19081 agggaagtaa ggtgagggcg agaaaaatcc gtttgagatt caaggtgatc attcctttac
    19141 caatgagtag ccgatgcgcc attataggtc ctggatgtgg gattttttta tcctgttagc
    19201 gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg ttctgttgcg
    19261 tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa gaggacattc
    19321 gccttggaca cacccagtag atactggctc actatcctgt catccaggat caactcctaa
    19381 ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt ttcccgtcgc
    19441 gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc caccgaactg
    19501 tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac tggaaaataa
    19561 ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag tatggattga
    19621 tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg gccagagcct
    19681 ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg aagacgatga
    19741 cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg ccggtaactc
    19801 cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc gtgaactgcc
    19861 cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg gtaacgccta
    19921 cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg aaattgaaaa
    19981 tatctatagc gacctggagc aattgagccg ggtgattatg gaagggcatc agggcgatga
    20041 gtacgacgag gcgctctcca ctctggcgga actggaagat atcggctgga aagtgcgcct
    20101 gtgtctgatg gatacccagc gcgcgctcaa cttcctggta cgtaaagcac gcttaccggg
    20161 tgggcaactg gagcaggcgc gtgaaatcct gcgagatatc gaatccctgc tgccgcataa
    20221 cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt ttatcaacat
    20281 cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc tgccgccaac
    20341 gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga agtggagttt
    20401 cggctaccca gg
//
LOCUS       DAZKIF010000091        19936 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZKIF010000091 DAZKIF010000000
VERSION     DAZKIF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.091"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            38..562
                     /gene="lpfA1"
                     /locus_tag="ACHOTW_003214"
     CDS             38..562
                     /gene="lpfA1"
                     /locus_tag="ACHOTW_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFP9845266.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            627..1313
                     /gene="lpfB"
                     /locus_tag="ACHOTW_003215"
     CDS             627..1313
                     /gene="lpfB"
                     /locus_tag="ACHOTW_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFP9845267.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGALNITLPAGVGSVSKIKYNTINDFGS
                     SAEMITKNVD"
     gene            1342..3915
                     /gene="lpfC"
                     /locus_tag="ACHOTW_003216"
                     /pseudo
     CDS             1342..3915
                     /gene="lpfC"
                     /locus_tag="ACHOTW_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
     gene            3930..4985
                     /locus_tag="ACHOTW_003217"
     CDS             3930..4985
                     /locus_tag="ACHOTW_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9845268.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            4990..5520
                     /gene="lpfE"
                     /locus_tag="ACHOTW_003218"
     CDS             4990..5520
                     /gene="lpfE"
                     /locus_tag="ACHOTW_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFP9845269.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5775..7466
                     /gene="eptB"
                     /locus_tag="ACHOTW_003219"
     CDS             5775..7466
                     /gene="eptB"
                     /locus_tag="ACHOTW_003219"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP9845270.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7558..7634
                     /locus_tag="ACHOTW_003220"
     tRNA            7558..7634
                     /locus_tag="ACHOTW_003220"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7592..7594,aa:Pro,seq:cgg)
     gene            8379..9986
                     /gene="dppA"
                     /locus_tag="ACHOTW_003221"
     CDS             8379..9986
                     /gene="dppA"
                     /locus_tag="ACHOTW_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP9845271.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10137..11156
                     /gene="dppB"
                     /locus_tag="ACHOTW_003222"
     CDS             10137..11156
                     /gene="dppB"
                     /locus_tag="ACHOTW_003222"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP9845272.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11166..12068
                     /gene="dppC"
                     /locus_tag="ACHOTW_003223"
     CDS             11166..12068
                     /gene="dppC"
                     /locus_tag="ACHOTW_003223"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP9845273.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12079..13062
                     /gene="dppD"
                     /locus_tag="ACHOTW_003224"
     CDS             12079..13062
                     /gene="dppD"
                     /locus_tag="ACHOTW_003224"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP9845274.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13059..14063
                     /gene="dppF"
                     /locus_tag="ACHOTW_003225"
     CDS             13059..14063
                     /gene="dppF"
                     /locus_tag="ACHOTW_003225"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP9845275.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14093..15364)
                     /locus_tag="ACHOTW_003226"
     CDS             complement(14093..15364)
                     /locus_tag="ACHOTW_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP9845276.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            15840..15947
                     /locus_tag="ACHOTW_003227"
     CDS             15840..15947
                     /locus_tag="ACHOTW_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9845277.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16034..17713)
                     /gene="bcsG"
                     /locus_tag="ACHOTW_003228"
     CDS             complement(16034..17713)
                     /gene="bcsG"
                     /locus_tag="ACHOTW_003228"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP9845278.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17710..17901)
                     /gene="bcsF"
                     /locus_tag="ACHOTW_003229"
     CDS             complement(17710..17901)
                     /gene="bcsF"
                     /locus_tag="ACHOTW_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP9845279.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(17898..19469)
                     /gene="bcsE"
                     /locus_tag="ACHOTW_003230"
     CDS             complement(17898..19469)
                     /gene="bcsE"
                     /locus_tag="ACHOTW_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP9845280.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            19742..19930
                     /gene="bcsR"
                     /locus_tag="ACHOTW_003231"
     CDS             19742..19930
                     /gene="bcsR"
                     /locus_tag="ACHOTW_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP9845281.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         4988 a         4852 c         5260 g         4836 t
ORIGIN      
        1 ttttcttttt aatgtaattt tttaaatgga gtttttcatg aaaaaggtcg tttttgcctt
       61 aaccgcactg gcgttaactt ctggcactgt attcgcagca gagtctggtg acggcacggt
      121 taaatttaca ggcgagatcg tggattcacc ttgcgtactg tccgttgact ctcagaacca
      181 ggaagttgtg ttgggtcagg tacaaaaatc tgtatttgct gcggttggtg acaaatcccc
      241 ggcaaaacct ttcgaaatca aactggaaga ttgcgatacc accacgatga aaaaagccaa
      301 tgtgagcttt agtggtgttg gtgatgcaga taaatctgat ctgatttctg ttagcactga
      361 agcgggcgcg gccaaaggtg ttggtatcgg tatttatgat aacagcaata ccctggtcgc
      421 tcttaacggt ggtaaagcaa gtgttgatct gagtaaaggc cagacggttc tctactttac
      481 cgctaactac gtttctactc tggcaacggt aacgaccggt tacggcaatg cgcaggtcga
      541 tttcaacctg tcttacgagt aatcgtccgt tttacaaatg gcggcgtaat tgccgccatt
      601 agtcgtcaaa gttcatggat cgaatgatga aaagtaaatt tgttgcgttg gctctctctc
      661 tgttcctttc acagtcagtt ctggcgggtg gcgtcggact tagctctact cgcgtaattt
      721 atgatggcag taagaaagaa gcctcgctga ccgtacagaa taaaagtaag acggaagagt
      781 ttcttatcca atcctgggtg gatgatgccg cgggcagcaa aaaaacaccg tttattatta
      841 cgcccccact attcaagctt gatccagaaa agaacaatat tttgcgtatt gtgaatatta
      901 cgcccggcct gcctcaggat cgcgaatcgg tctactgggt taatgtgaaa gccattccat
      961 ctaaaagtga cgacagcgaa aacaaaaatg ttttgcagat tgccgttcgc acccgaatca
     1021 aactttttta tcgcccggca ggtttaaagg gtgatgtaaa aaccgcgcca aatgaactgc
     1081 gatttacgcg taacggtaat cagctccgcg ttgataatcc gacagttttt aatattacct
     1141 ttaatcaatt ttttgcgaat gacaaagaaa ttgaaaaagc aggcatggta cccgctaaag
     1201 gggcattaaa tatcacatta cccgccgggg taggtagcgt ttctaaaatt aaatataaca
     1261 ccatcaatga ctttggatcg tcagcggaaa tgatcactaa aaatgttgat tgattaatat
     1321 cttttcagga gaaatagctc aatgtcacgg aagaccgttt cacgtacttt ttcttccttt
     1381 tctatctctg tggtggcggt tgctgttgcc agcacgtttt ctgcacatgc cggaaagttt
     1441 aaccccaaat tcctggagga cgttcagggt gtgggccagc atgtcgattt aacgatgttc
     1501 gaaaaaggcc aggaacagca gttacccggc atctatcgtg tctctgttta cgtcaatgaa
     1561 cagagaatgg aaacccgcac actggaattt aaagaggcca cagaagcaca gcgcaaagcg
     1621 atgggggaat cccttgtgcc ttgccttagc cggacgcaac tggcagagat gggcgtgcgc
     1681 gttgaaagct ttccggccct caatctggtg tctgccgaag cctgcgtacc gtttgacgaa
     1741 atcattcccc tggcctcaag ccatttcgac ttcagtgaac aaaagctggt attaagtttc
     1801 cctcaggcgg cgatgcacca ggtggcgcgc ggcacggtgc cggaatcttt atgggacgaa
     1861 ggtatccctg cgttgctgct ggattacagt tttagcggga gcaacagtga atatgactcg
     1921 acggggtctt ccagcagtta tgtcgatgat aatggcactg ttcatcatga tgatggtaaa
     1981 gatacgctaa agagcgacag ctactacctc aacttacgca gcgggctgaa ccttggcgct
     2041 tggcggttac ggaattacag tacctggagc cattccggtg gcaaagcgca gtgggataat
     2101 atcggtacgt ctttgagtag ggccatcatc cctttcaaag cgcaattaac aatgggggat
     2161 acggcgaccg cgggggacat ttttgacagc gttcagatgc gtggcgcgat gttagcctct
     2221 gatgaagaga tgctgcccga cagccagcga ggctttgcac ccatcgttcg agggatcgcc
     2281 aaaagcaacg ccgaagtcag tattgaacaa aacggctacg ttatttatcg cacctatgtg
     2341 cagcctggtg cctttgaaat aaacgatctt taccccactg caaacagcgg tgatctcacg
     2401 gtcattatta aagaagcgga tggcagtgaa caacgcttta tttagccatt ctcatcggtg
     2461 ccaattttcc agcgtgaagg ccatctcaaa tatagctttg ccgcaggtga atatcaggcc
     2521 gggaattatg acagcgcctc gccgcgtttc gggcagcttg atctgatcta cggtttaccg
     2581 tgggggatga cggcctacgg cggcgtatta atctctaata attacaatgc atttacatta
     2641 gggataggga aaaactttgg ttatatcggg gcgatttcca ttgatgtgac gcaggctaaa
     2701 agcgaactga ataacgatcg cgatagccag ggacaatctt atcgtttctt atattccaag
     2761 agcttcgaaa gcggcaccga tttccgcctt gcgggctatc ggtactctac cagcggtttc
     2821 tataccttcc aggaagccac cgatgtgcgc agtgacgctg acagcgacta taaccgttat
     2881 cacaagcgca gcgaaataca gggtaacctg acgcagcaat taggggccta tggctctgtt
     2941 tatttaaatt taacgcagca ggattactgg aacgacgcag gtaaacagaa cacggtatcg
     3001 gcgggttaca acggacgtat tggcaaggtc agttacagta ttgcatatag ctggaataaa
     3061 agccctgaat gggatgaaag cgatcgcttg tggtctttca atatttccgt tccactaggc
     3121 cgggcctgga gtaactatcg cgtcacgacc gaccaggatg gtcgtaccaa tcaacaggtt
     3181 ggggtcagcg gaacgctgct tgaggatcgc aacctgagct acagtgtcca ggaaggctac
     3241 gccagcaacg gtgtgggtaa cagcggtaac gctaacgttg gctatcaggg tgggtccggt
     3301 aatgtcaacg taggctatag ctacgggaaa gattaccggc agctcaacta cagcgttcgc
     3361 ggcggcgtga tagttcatag cgaaggcgtg acgctttccc aaccgctagg cgaaaccatg
     3421 acgctcatct ccgtacccgg tgcgcgcaat gcccgcgtgg tgaataacgg cggcgttcag
     3481 gttgactgga tgggtaacgc gatcgtgcct tatgccatgc cgtatcgtga aaacgaaatc
     3541 tcactgcgta gcgattcgtt gggtgacgat gttgacgttg aaaatgcgtt ccagaaagtg
     3601 gtgccaacgc gtggagcgat tgtcagagcg cgttttgata cccgcgttgg ttaccgcgta
     3661 ttaatgacgc tgcttcgttc cgcgggcagc ccggtgccct ttggagcaac ggcaacgcta
     3721 atcaccgata aacaaaacga ggtgagcagt atcgttggtg aagaaggaca gctctatatt
     3781 agcggaatgc cagaggaagg acgggtattg attaaatggg gtaatgacgc gtcgcagcaa
     3841 tgcgtggcgc cttataaatt atccctggaa ttaaaacagg gcggaattat tcctgtttcg
     3901 gccaattgcc agtaagcaag gagagcgaaa tgaaagcggc aatagcatta tccttattag
     3961 gatgcgtatt cggattttct ggcaaggcat ttgccggaga tgcgtggggg ccatgtaccc
     4021 ccgcggatgg cacgacttat cactataatg tcgatgtgga tgttggtata cctgatgcgg
     4081 caaaaaacgt cgcaggaacg gttttaccgg atgtccttaa ctggtctaat ggacaaaacg
     4141 tatcgctaat ttgtgaatgt cctgattctt ataagaatga aaaagatact ctagtgcagg
     4201 gggtgagtat gcttccccct tctggccgta cggttgacag tatgaaatat tatactttga
     4261 cggaggaact ggaggttgcg acgaacattc gcatcagtac cagtgtgtat gggtttgttc
     4321 ctttcaaaaa ccagcaggcg ctgcaaacaa ccggatgcaa caaagtcatt actacgccct
     4381 atatgggcgg cgcaggtcta ctttcctttg ctattactaa accctttatc ggcgattccg
     4441 tcatccctct gacgttaatt gccgaactgt atgcctcgaa aacaaataaa gattacggaa
     4501 caatacccat atcttccgta tctattcagg ggcgtgtcac cgtaacccag gattgcgaaa
     4561 ttaaacccgg cacggtgctg gatgtgccat ttggtgaatt cccctcctcg gcgtttaaaa
     4621 acaggcaagg gcaaatgcct gaaggtgcga cagagcaaga aattaacctc agcttcgatt
     4681 gcaacaatat ctctgatggt atcaaggttg cgttgcgcct tgagggggca accaatgctg
     4741 acgatccccg agcggtggat atgggcaacc ccgatattgg cgtactggtg aaagattcca
     4801 gcggcaaaat tttggtaccg aatgattcaa gcagcaccac gttattaaat ttgagcagcc
     4861 tggattctaa aactcaccgc aatgccgcga ttcgactgct ggctttaccg attagcacga
     4921 cgggtaaagc gcctaaaggc gggacgtttg aaggcgtaac gaccatatat cttgaaatgg
     4981 agtgatgcca tgaagttcaa acgattgctg catagcggca tcgccagttt gagtctggtt
     5041 gcctgcgggg tgaatgcggc gacggatctt ggcccggcag gggatattca tttctccatc
     5101 actatcacca ctaaagcttg cgagatggaa aaaagcgatc tcgaagtcga tatgggaaca
     5161 atgacgctgc aaaaacctgc ggcagtcggt acggtgttga gcaagaaaga tttcaccatt
     5221 gaactcaaag agtgcgatgg gatatccaaa gcgaccgttg agatggacag tcagtcggac
     5281 agcgatgatg attccatgtt tgcccttgag gctggtggcg caacgggtgt tgcgttgaag
     5341 atagaggacg ataaaggaac gcagcaagtt cccaaaggct ccagcggaac gccgattgaa
     5401 tgggcgattg atggcgaaac cacgtcgctt cactaccagg cgagttatgt ggtcgtcaac
     5461 actcaggcca ctggtggcac agcgaatgcc cttgtaaatt tttccatcac ctatgagtaa
     5521 gttactgggc cagcatcctg gcccaaccta tctctgaata gtcgttaatc cgttaaaatg
     5581 gtctattccg gacttgcact tttgtaaatt tgtgcttcat gcacactctt tccccacact
     5641 ttttcccttt gctgtggtct acttattcgc gcgtgtagat tttacttatc tgactacctc
     5701 cgcacttttc ccctgctggg cctgaaaagc cgctaagcag ggtgttatca cctgtttgtc
     5761 cagggtttgt ttgcatgaga tacatcaaat cgattacaca gcagaagctg agctttttgc
     5821 ttgcaatcta tattggcctt tttatgaatg gcgcggtttt ttaccgccgc ttcggcagct
     5881 atgcgcacga ttttaccgtc tggaaaggca tttctgctgt tgttgaactg gccgccaccg
     5941 tactggtgac cttcttttta ctacgtcttc tttcgctgtt tggccgccgc agctggcgta
     6001 ttctggcatc gctggttgtg ctcttttccg caggtgccag ctattacatg accttcctta
     6061 atgtggtcat tggttatggc atcatcgctt ccgtcatgac caccgatatc gacctgtcaa
     6121 aagaagttgt tggtctgaac tttattctct ggttaatcgc cgttagcgca ttgcctctta
     6181 tccttatctg gaataaccgc tgtcgctaca ccttgctccg acaactgcga accccagggc
     6241 agcgtattcg cagcctggcg gtcgtcgtac tggcgggaat tatggtttgg gcaccgattc
     6301 gtttgctgga tatccagcag aagaaagtgg agagggcgac cggcgttgat ttgccgagtt
     6361 atggcggtgt cgtagcgaac tcttatctgc catcaaactg gctttctgcg ttggggctgt
     6421 acgcctgggc gcgggtcgat gaatcttccg ataataattc attgcttaac ccggcgaaga
     6481 aattcactta tcaggcaccg caaaatgtta atgatactta tgtcgtgttt atcatcggtg
     6541 aaaccacgcg ttgggaccat atgggcattt tcggctatga gcgtaatacc acgccgaagc
     6601 tggcccagga gaaaaatctg gcggcgttcc gtggttactc ctgtgatacc gcaaccaaac
     6661 tctctctgcg ttgcatgttt gtacgtcagg ggggcgcgga agataatccg cagcgcacat
     6721 taaaagaaca gaacattttc gcggtactga agcagttagg attcagttct gacctctacg
     6781 ctatgcagag cgaaatgtgg ttctacagca atacgatggc ggacaatatt gcttaccgtg
     6841 aacagattgg tgcggagcca cgtaaccgtg gcaagccggt agatgatatg ttgctggtag
     6901 acgaaatgca gcaatcgctg gggcgcaacc cggatggtaa gcatctaatc attctgcata
     6961 ccaaaggttc gcactttaac tacacccagc gttacccgcg cagctttgcg cagtggaagc
     7021 cggaatgtat tggtgttgat agcggatgta ccaaagcgca gatgatcaac tcctatgaca
     7081 actcggtgac ctatgtggat cactttatct ccagtgtgat tgatcaggtt cgcgataaga
     7141 aagcgattgt gttctacgca gctgaccacg gcgagtcaat taatgaacgc gagcacctgc
     7201 acggcacgcc gcgtgaactg gcaccgccgg agcagttccg cgtaccgatg atggtctgga
     7261 tgtcagataa atatctggaa aatccggtca atgcgcaggc gtttgcgcag ctgaaaaaag
     7321 cagccgacat gaaagtgcca cgccgtcacg tagagctgta cgacaccatc atgggttgtc
     7381 ttggctatac ttcaccggat ggtggaatta acgaaaacaa caactggtgt cacatcccgc
     7441 agacaaaaga ggcagcggct aactaagagg cctgctgact ttctcgccga tcaaaaggca
     7501 ttttgctatt aagggattga cgagggcgta tctgcgcagt aagatgcgcc ccgcattcgg
     7561 tgattggcgc agcctggtag cgcacttcgt tcgggacgaa ggggtcggag gttcgaatcc
     7621 tctatcaccg accaaattcg aaaagcctgc tcaacgagca ggcttttttg catctgcagc
     7681 ccatgaggaa gagaacctcc gggggcaggg aggttcgact cgacgtaggc ctgataagac
     7741 gcgtcagcgt cgcatcaggc aacggcaccg cacttattgt cggatgcggc gtgaacgcct
     7801 tattcgacct acacgccaca catttccctg ccaaatttgc gccccatatc tcactatcca
     7861 ttaacggatt tgtgacagaa accattgtct ttttttatat ttgcttaaat ctttttttgc
     7921 gttacttatg gctggcgctg gcattttccg ctgtgcgttt tagcgttcat ggtattaatc
     7981 gctcagatat ccaccttttc ctcagatttt tttcactaaa tgcataaaac ttaatcacca
     8041 aatgttgcta aatattaagc tgattgttct ggtcatgttg gagtagcaca aatttctctg
     8101 ctgcaaatag ctgtttgaag tttttttaat ctttgtttgt ggattctcac cgttggtgta
     8161 aatcccggtt ggctgtattg acgttttcac attctgttga cagattgtag gtcacgaggg
     8221 gcattttatg gaggatccgc actgttacac tgatgttaat tagtacggca tccccacctc
     8281 ataacgttga cccgaccggg caaaaaacaa aaaaggtcag gcagcgacaa cccactgcaa
     8341 agggttaaaa caacaaacat cacaattgga gcagaataat gcgtatttcc ttgaaaaagt
     8401 cagggatgct gaagcttggt ctcagcctgg tggctatgac cgtcgcagca agtgttcagg
     8461 ctaaaactct ggtttattgc tcagaaggat ctccggaagg gtttaacccg cagctgttta
     8521 cctccggtac cacctatgac gcctcttccg taccgcttta taaccgccta gttgaattta
     8581 aaatcggcac caccgaagtg attccgggcc tcgctgaaaa gtgggaagtc agcgaagacg
     8641 gtaaaaccta taccttccat ctgcgtaaag gtgtgaagtg gcacgacaat aaagaattca
     8701 aaccgacgcg cgaaatgaat gccgatgacg tggtgttctc gttcgatcgt cagaaaaacg
     8761 cgcaaaaccc gtaccataaa gtttctggcg gcagctacga atacttcgaa ggcatgggct
     8821 tgccggagct gatcagcgaa gtgaaaaagg tggacgacaa caccgttcag tttgtgctga
     8881 ctcgcccgga agcgccgttc ctcgctgacc tggcaatgga cttcgcctct attctgtcaa
     8941 aagaatatgc tgatgcgatg atgaaagccg gtacaccgga aaaactggac ctcaacccaa
     9001 tcggcaccgg tccgttccag ttacagcagt atcaaaaaga ttcccgtatc cgctacaaag
     9061 cgtttgatgg ctactggggc accaaaccgc agatcgatac gctggttttc tctattaccc
     9121 ctgacgcttc cgtgcgttac gctaaattgc agaaaaatga atgccaggtg atgccgtacc
     9181 cgaacccggc agatatcgcc cgcatgaagc aggataaatc tatcaacctg atggaaatgc
     9241 cggggctgaa cgtcggttat ctctcgtata acgtgcagaa aaaaccactc gatgacgtga
     9301 aagttcgcca ggctctgacc tacgcggtga acaaagacgc gatcatcaaa gcggtttatc
     9361 agggcgcggg tgtatcagcg aaaaacctga tcccgccaac catgtggggc tataacgacg
     9421 acgttcagga ctacacctac gatcctgaaa aagcgaaagc cttgctgaaa gaagcgggtc
     9481 tggaaaaagg tttctccatc gacctgtggg cgatgccggt acaacgtccg tataacccga
     9541 acgctcgccg catggcggag atgattcagg cagactgggc gaaagtcggc gtgcaggcca
     9601 aaattgtcac ctacgaatgg ggtgagtacc tcaagcgtgc gaaagatggc gagcaccaga
     9661 cggtaatgat gggctggact ggcgataacg gggatccgga taacttcttc gccactctgt
     9721 tcagctgcgc cgcctctgaa caaggctcca actactcaaa atggtgctac aaaccgtttg
     9781 aagatctgat tcaaccggcg cgtgctaccg acgaccataa caaacgcgtt gaactgtaca
     9841 agcaagcgca ggtggtgatg cacgatcagg ctccggcact gatcatcgct cactccaccg
     9901 tgtttgaacc ggtacgcaaa gaagttaaag gctatgtggt tgatccatta ggcaaacatc
     9961 acttcgaaaa cgtctctatc gaataattaa aagccatacc cggtggtgct gtgcctctgc
    10021 gtgcagcgcc atccggcagc aatacttcat tccctgcccg acctgggcgg gaattgattt
    10081 gtgagcaata cagacacgca gttccaggct gcgggtcact acagagaatc cgggttatgt
    10141 tgcagtttat tctccgacgt ttgggactcg tcatccccac gtttatcggt attacccttc
    10201 tcacatttgc ctttgtccac atgatcccgg gcgatcctgt gatgatcatg gcgggcgaac
    10261 gtgggatctc cccagagcgt cacgcgcagt tgctggctga actcggctta gataaaccga
    10321 tgtggcagca gtatctccat tacatttggg gcgttatgca tggcgatcta ggcatttcaa
    10381 tgaaaagccg aattccggta tgggaagagt tcgtgccgcg ctttcaggcc acgctggaac
    10441 ttggcgtctg cgcgatgatt tttgcgaccg cggtcggcat tccggttggt gtgctggccg
    10501 cggttaaacg cggttccatt ttcgatcaca cagcggttgg cctggcgctg acaggttatt
    10561 caatgcctat cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg cactggaacc
    10621 tgacacccgt ctccggtcgc gtgagcgata tggtgttcct cgatgattcc aatccgttaa
    10681 ccggttttat gctgatcgac accgccatct ggggtgaaga cggtaacttt atcgatgccg
    10741 tcgcccatat gatcttgccc gccattgtgc tgggtactat tccgctggcg gtcattgtgc
    10801 gtatgacacg ctcctcgatg ctggaagtgc tgggtgaaga ttacatccgc accgcgcgcg
    10861 ccaaagggtt gacccgcatg cgggtgatta tcgtccatgc gctgcgtaac gcgatgctgc
    10921 cggtagtgac cgttatcggc ctccaggtgg gaacattgct ggcgggggcg attctgaccg
    10981 aaaccatctt ctcgtggccc ggtctggggc gctggttgat tgacgcactg caacggcgcg
    11041 attatccggt ggtgcagggc ggcgtattgc tggtggcgac gatgattatc ctcgtcaacc
    11101 tgctggtcga tctgctgtat ggcgtggtga acccgcgtat tcgtcataag aagtaagggg
    11161 tcatcatgtc acaggttact gaaaataaag tgattagcgc gccggtaccg atgacccctt
    11221 tacaggagtt ctggcactat tttaaacgca acaaaggcgc ggtcgtcggg ctggtttacg
    11281 tcgtcatcgt gctgtttatc gcgatctttg ccaactggat tgctccctat aacccggcgg
    11341 aacagttccg cgatgcactg ctcgccccgc cagcctggca ggaaggcggc agcatggcgc
    11401 acttgctggg taccgatgat gtaggccgtg atgtgctgtc gcggctgatg tacggcgcgc
    11461 gcttgtcgct gctggttggc tgtctggtgg tggtgttatc gctgattatg ggcgttattc
    11521 tcggcctgat cgccggttac ttcggcggcc tggtcgataa catcattatg cgcgtggtcg
    11581 atatcatgct ggcgctgcca agtctgctgt tggcactggt gctggtggcg attttcggcc
    11641 cgtcgattgg taacgccgcg ctggcgctga cctttgttgc cttgccgcac tatgtgcgct
    11701 taacccgcgc cgccgtgctg gtggaggtga atcgcgatta cgtcaccgct tctcgcgtgg
    11761 caggagccgg ggcgatgcgc cagatgttta ttaacatctt cccgaactgc cttgcaccgc
    11821 tgattgttca ggcgtcgctc ggtttctcta acgccattct cgatatggct gctcttggtt
    11881 tcctcggcat gggggcgcag ccgccaacgc cggagtgggg caccatgctc tccgacgtgt
    11941 tgcagttcgc gcaaagcgcc tggtgggtcg tgaccttccc gggtctggcg atcctgctga
    12001 cggtgctggc atttaacctg atgggtgacg gtctgcgtga cgcgctcgat cccaaactga
    12061 agcagtaaga ggttcgagat ggcgttatta aatgtagata aattatcggt gcatttcggc
    12121 gacgaaagcg caccgttccg cgccgtagac cgcattagct acagcgtaaa acagggcgaa
    12181 gtagtcggga ttgtgggtga gtctggctcc ggtaagtcgg tcagttcgct ggcgattatg
    12241 gggctgattg attatccggg ccgcgtgatg gcggaaaagc tggagtttaa cggtcaggat
    12301 ttgcagcgta tctcggaaaa agagcgccgc aacctggtgg gtgccgaagt ggcgatgatc
    12361 ttccaggacc cgatgaccag ccttaacccg tgctacaccg tgggtttcca gattatggaa
    12421 gcgattaagg tgcatcaggg cggtaataag agcactcgtc gtcagcgggc gattgacttg
    12481 ttgaatcagg tcggtattcc cgatccggct tcgcgtctgg atgtttaccc gcatcagctt
    12541 tccggcggca tgagccagcg cgtgatgatt gcgatggcca ttgcctgtcg gccaaaactg
    12601 ctgattgccg atgaaccaac cactgcgctg gacgtgacca ttcaggcgca aatcattgaa
    12661 ctactgctgg agctacagca gaaagagaac atggcgctgg tgttaattac ccatgacctg
    12721 gcgctggtgg cggaagcggc acataaaatc atcgtgatgt atgccggtca ggtggtggaa
    12781 acgggcgatg cgcacgccat cttccatgcg ccgcgtcacc cgtatactca ggcattgctg
    12841 cgtgcgctgc cggaatttgc tcaggacaaa gaacgtctgg catcgttgcc tggcgttgtt
    12901 cccggcaagt acgaccgccc gaacggctgc ttgcttaacc cgcgctgccc ctatgccact
    12961 gacagatgtc gcgctgaaga accggcgctg aatatgctcg ctgacgggcg tcagtccaaa
    13021 tgccattacc cacttgatga tgccgggagg ccgacactat gagtacgcaa gaggccacct
    13081 cgcaacaacc gctgttgcag gctatcgacc tgaaaaaaca ttatccggtg aagaaaggta
    13141 tgttcgctcc ggaacgtctg gtgaaggcgc tggacggcgt ttcgtttaac cttgaacgtg
    13201 gcaaaacgct ggcggtagta ggtgaatctg gctgcggtaa atcgaccctc ggtcggttgc
    13261 tgacgatgat tgaaacgccc accggcggcg agctgtatta ccaggggcag gatctgctta
    13321 agcacgatcc gcaggcgcag aagctgcgtc ggcagaaaat ccagattgtc ttccagaacc
    13381 cttacggttc gctgaatccg cgtaaaaaaa tcgggcaaat tcttgaagag ccgctgctga
    13441 tcaacaccag cttaagcaaa gaacagcgtc gggaaaaagc cctgtcgatg atggcgaaag
    13501 tcggcctgaa aaccgagcac tatgaccgct atccgcatat gttctccggc ggtcagcgtc
    13561 agcgtatcgc cattgcccga ggtctgatgc tcgacccgga cgtggtgatt gccgatgaac
    13621 cggtctccgc gctggacgtc tcggtgcgtg cacaggtgct caacctgatg atggatttgc
    13681 agcaggaact ggggctttct tatgtcttca tctcccacga cctgtcggtg gtggagcaca
    13741 ttgctgatga agtgatggtg atgtacctgg gccgctgcgt ggagaaggga acgaaagacc
    13801 aaatcttcaa taacccgcgc catccgtaca ctcaggcgct actttccgcg acgccgcgcc
    13861 tgaatccgga cgatcgccgc gagcgcatca aactcaccgg tgaactgcca agcccgctga
    13921 atccaccgcc gggctgcgcg tttaacgccc gctgtcgtcg gcgcttcggc ccctgcaccc
    13981 agttgcagcc gcagctaaaa gactacggcg gtcaactggt agcttgtttt gctgttgatc
    14041 aggatgaaaa tccgcagcgt taactcacaa aaccggggcc atgcgctccg gtttactcaa
    14101 tcagcttcaa cagtggcgac aagcatagca aaatgccgta aagcagaatc agccaggttt
    14161 taaatccgcg aagtttttcc agttgtgcga ctttatagat caggaaaaac ggaatgaggc
    14221 acgacacaat gccatataac gggcttccca actgaaagaa caccagcacc gatacacgaa
    14281 acgaaaccca aatcgtcaac gtaatgacga taaaagcgca gatcgccaga gtcagcacgc
    14341 gtgagttaat tttcttggta tcaataattc ggcttaacag attgagaata atgcctttaa
    14401 tggcctcgtg gaaaccgaga taaatgccaa agaatgcggt cagtacggca aagatattca
    14461 gcaccgtaga ggtgatatga atgatatgcc cagggatcac ctgcgcggcc agtgccagtg
    14521 ccgagatatt ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag aaggtaaacg
    14581 aaaaggcaaa aaacagtatc accgcgatga gcgtgatata actaattcgg tgggtacgca
    14641 gcgcgagccg ggttgccagt actttatccg cttcccgttt acgataggca atattcattg
    14701 ggttaagtac ctgaataaat actgcagaaa agaagcaaaa tggaatggta agcaggacat
    14761 cgcgaaaaaa gacggaggct tgcgggaagg cggttatatt ggcgaaattc cagtgcggga
    14821 tcatcgtaaa accgaacacg acaataatcc ctactttgac caccaccatt ggcccagaaa
    14881 tcttaaacag taatcgttca ccaccagacg caatcgctac cagtacggcg aaaatagcga
    14941 ctttataaag tagagattgt gaaagatcgg catcggttaa accgaaggtt ttcaggtacg
    15001 aggcgctgtc gaaaaccacg gagagagagt agataaaaat cccgtggata atcatcaaaa
    15061 agtagataac cccgaggaaa attccccagt tcttccccag gtaatgactg ataatatcgg
    15121 tgtagtcatt acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc tgcactaccc
    15181 aggtggcagg ataagcaatg atcgctgcgg taataaatac ccaaatgccc ttcaagccaa
    15241 tttgtactgg catcagcacg gttccggcac caatcgccat gcctatgcat aataaaaccc
    15301 agccgaagtc gtagcgtgta aacggcaatt tctgatcgtg tttcggtagt gtgttgtgct
    15361 gcataaagtc ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc cagtttctct
    15421 tgcgcaacag gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa cagattcact
    15481 ggggaggtcg cgaggaggcg caattctaca aaattgcgca tttcactacc agtctggagt
    15541 cagcccggat atgaactgca actattgcaa attaagaaat taagctggaa ggcatcacgc
    15601 aatgaggaag attgccgagt gctgacagta aaaagcaatc gtaaaattag cggcaagccg
    15661 gaagggttcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca ttggttgctg
    15721 gagagagaaa acccccgcac gttgcaggta tgcacctgac aacaccacgg gggctaatct
    15781 tgaacctaga ccactcaaga atagccgcga aacgttgtca ttacaacaca ggcggctata
    15841 tgacgctcgc agagctgggc atggccttct ggcatgattt agcggctccg gtcattgctg
    15901 gcattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt gtcatgcggg
    15961 cgtcaggctg ccgtaatggc aatttgcgcc cggaccagac cgcagggggg aaactctgcg
    16021 gcctttttcg tgcttactgc gggtaaggca cccagtcgcc gccgttcagg cgaacgtacg
    16081 gtttatcctg gtattgaata actactgcat ttgagttctc tgagaccggt gctgtttgtg
    16141 gcaacccact ggtgagtttt ttccagtcaa cattgtcttc ggtgaaaatc ttgccatcaa
    16201 gaacgcgaac caccagatcg gagatagcca ggaagctgct cggttggtcg atgacaatcg
    16261 gtgccccctg atgtggtgcc ttcatgccga agaatttcac cccaacgggg acgtcggtga
    16321 tagacgggct agggatatca cgtaggccag atacctgcat tctgtcgccc ttcagcgcgc
    16381 cgccgtgttc cggcaccacg accaccatca ctttacgacc cgatttctcc agttcagtaa
    16441 agaaggcgtc cagttcatca aagaatttct gcgcccgcgc tttgtaatcc gctgttttgc
    16501 tgacgcccgg ataatggttg ccgtcatgca gtggaagcgt gttgtagaac gtggcactac
    16561 ggctgttttt atctttttcg gtaacgtcca gccagcggtt aagcacggcg gtatcgtcat
    16621 aaaccggcga accatcaaag cccagcaaaa taaccggcag atttgtttga tccatcaatt
    16681 cagtctgcat gccgccattt tcgcgaactt ctttcaaaaa accgccgaac tggccgttat
    16741 gtcccatcat caggtgctgg gtaaagccca gtttcgacag gttatcaaac agatagcagt
    16801 cgttatttgc cggttgatac agattagtgt gcgaagtctg cccgcagctg gcgcgcagta
    16861 aacggatcgc cgccgggcca ctgtaggagg tggcggagtt aaagttcttg aactcaatat
    16921 cgaaatgcga ccacagtgga tgcgacatca acccagcggc ttctatatcc gaccaggaaa
    16981 gcgaacagat gttaatcacc agtagttcaa atggctgagc atcagcgggc agcgaagacg
    17041 ggaaggtcga tttacgtttc gcctccgcgt tatagaaatt attcagccag gcgttaaggt
    17101 tcgccgttgt tggcggtgca gtttgtgcgg gcatatcacc cactaccggt gcgccacccg
    17161 tcgccgcaac ggttgccgct gcgttaccac ccgtcgttgt tacagtggtc gtcggttgtc
    17221 cggctggcca caaggagaaa cttggtcccg ccagggtaag tacgttcagc catagcagta
    17281 tggcaaccac aaaaacggta atgcgaatcc attgtgacag gaataaccag gccactaata
    17341 aaacaaaaat ggccccaatc atctgccagt taataaagcg tgtgacaagg tcgattaaat
    17401 aatcggtact gaaccccgcc acctgcgaac cctggctcat tatgctttcc gggccaggca
    17461 accaggtgtc atgccagaac aaagcaaagc cgatcggcag ggcaatccag tggcgcaagc
    17521 gatgcaggct gtagcgcgga atgggcatca gcagaaacgc ggcaaacacc aaattgagga
    17581 gcggatggaa gttaagatat cccgcccaca acaggccgaa cttaaccaga aaatagaagt
    17641 tccagccgga aaggccgcgc cagtattgcc agagggaaga aggcatggcg gtattttgcg
    17701 taaattgagt cattttttgg ttgccctggc tttttccgtg cggcgtaacg tcccagccgg
    17761 tttaacataa cgaggtttag caaagaacaa acgtaaggtg tcgcgaatgc gtcgcaaaga
    17821 gtgccgcgcc agatagccca gcgggaaaaa tatcagtgcg caaacgacaa taatttcaat
    17881 gatatcgctg atggtcatca tgatgagcgc tccacagcat catctaacag tcgcatgggt
    17941 tctggtattc gtcgccagtg gcgaccatcg tgctcggcat tgatgaccgg tttagaactt
    18001 tgcgttaaag gcagcggcat gccccattgt tctggggcaa gcaggcgcat ctgcaccagc
    18061 tcggcactga tttgatcatc ttcaaaccag accatacggt ttgagaaaat gtcgccagta
    18121 ggcaatggga aaatatgatt caacgcggta tccagatcgt taatccgaca gaatgagaga
    18181 aacagcacca gccgattacc gccaatggtc atgatatcgc cggtacggtt agggcgacac
    18241 agcgtcaggg cttgttcaac gcggataccc ggtaccggac gtagggcaac cagaacgcct
    18301 ttaccgtggg caggtaatag agggttattc atcatgttgt tgacggcatt acagaacaca
    18361 tcccacttct ggaaaccacg cagtttgagc ggctgggtca ttgacagcaa ggtagtgata
    18421 tcttccggca catagcgact aaacttctgc ccttgcacgc tttcgatcat cgtcagacaa
    18481 cgggagagtg gcgcattcca cggaataacc atatttgcac cgcaggccaa taacaaacgt
    18541 tcatcggtgg cgcgcaggct agcggtattt tcccgcacga ggattttcat cgcactaccg
    18601 cgctggcgac gcagggtatg aatgctgcgg gccagtggct cgatttgcgc attttgctgt
    18661 aaagaaaaga ccaccgtcgc cgcctgagcg gtacgggctt cattgaacag gacttcgtta
    18721 ttgttgaaca gttgccagtg ttccgatagc ggcggcgcac cttccagtac agcaacatta
    18781 ctcagaatgc gtttttcgtc gctgcgtggt tggatctccg cctcttcgct ttgaactaat
    18841 gtccagatac cattttgttg ctgaacgcta agctgctgac gggcgctgac ccctttttcg
    18901 ttgcaccaga aggcaatatc cagcaaatgt tggtcgccct gaaaacgcaa actggcaaga
    18961 ccaaaaagtg aacggtattc ctcaagcaac aatgaaaatt gtttatcgtt attatttccg
    19021 ggattaatta ccaaaagcga acaatggttt aacctgctcc atttattcat tttatccaac
    19081 catgagcgca gccgctcggc aggaatgttt tgccatgcgt tatttgcgca aacaagaata
    19141 aaaaggtaat tatggggatc aatagaacac tgcaaatcac gggccaaata gtatagacct
    19201 ttttcatgat ttagcattga aaataatttt attttttccg gcccttgaga atcatctaat
    19261 tgaaagattt tcgccggatc gctgtccatg ctaatgaccg cgacgtgtgc ggtttcagcc
    19321 tgggatgcaa ttgtttgatt cgccagactg atagcatctt catggcgatc gacgttaaac
    19381 caccagacgc cgcctgctgg catatgtcgc agctcatccc ataatgatga gataccgata
    19441 gagaatacag ggtccacaat gtccctcatg tcaccatttt gtctgattat cagtttacta
    19501 gcgaaagcta agaaataaac ctaccattga aattaaaact tatcagattt agcatgtgaa
    19561 tcaatttagc aggtcacaag acgtgtgatt ttagtctttt cgtttttaac tttttctgtt
    19621 gcgtaacgga aagtcaaaaa gtgagcaaat tcccgtctcg ccgtaaacaa taaaccggcg
    19681 aaatgctcac ggttagaatt gtctcatgaa cggtacggtt atttcatagg gatcaagcaa
    19741 aatgaataac aatgaaccag atactctgcc tgatcccgcg ataggctata tcttccagaa
    19801 tgatattttg gcgttaaagc aggcattttc actgcctggt attgattatg ccgatatttc
    19861 ccaacgcgaa cagttggccg cggcattaaa acgctggccg ttgctggcag agtttgcgca
    19921 acaaaagtag gggatt
//
LOCUS       DAZKIF010000092        19693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZKIF010000092 DAZKIF010000000
VERSION     DAZKIF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.092"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..563
                     /locus_tag="ACHOTW_003232"
     CDS             <1..563
                     /locus_tag="ACHOTW_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9845282.1"
                     /translation="VELGAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAE
                     LVSQTYKLPHTQFEQIANLYEMQFDDGRTCAFHTDAQKAEQWLQACDGNRVQEYVKLE
                     RLQNALSGNSPVTPDGWISCSERMPDTKTAVLVAVEFDRKGDWRMKWATYIPGHPDAN
                     DGWIIPGASWKPSHWMPLPEPPQEVN"
     gene            563..958
                     /locus_tag="ACHOTW_003233"
     CDS             563..958
                     /locus_tag="ACHOTW_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845283.1"
                     /translation="MENEGDNIITLVQPKRDEEKLLNITVTGRKNYTQQSCKHRAIEV
                     HEQDHVILCLQCGCVVDPFQYVLRCANDGEAVVREIRQLHNRHDQLRESVASLEREEK
                     NTKARLRAARTAILYAENDLKNIEQKVNQ"
     gene            1249..1404
                     /locus_tag="ACHOTW_003234"
     CDS             1249..1404
                     /locus_tag="ACHOTW_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9845284.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1524..1868
                     /locus_tag="ACHOTW_003235"
     CDS             1524..1868
                     /locus_tag="ACHOTW_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFP9845285.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSIWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            complement(2419..10800)
                     /locus_tag="ACHOTW_003236"
     CDS             complement(2419..10800)
                     /locus_tag="ACHOTW_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFP9845286.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDIVGKGLKATGKAVSDGAKATDEWLTGK
                     MSPGAVRALNTPMTEGYNDSAVWVAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTVSAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMGVSPWEGVADATIEGAAFGAAMGAPFGAVAGYRGRRQAAEETAMRDAETVQQDDAA
                     PQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     TKRQAVMNELKAKPRSELLEEYRRLSQKEGRTETEEQQFQAIREVIRPQQEVTPEAQS
                     QPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMG
                     SDVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGR
                     QGETLEGDMVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPEL
                     AQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGA
                     AKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDI
                     PAKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLP
                     PSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAK
                     VWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSAS
                     DARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGFR
                     GVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAA
                     HYESGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRR
                     IFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDKVRSKPYWSTDVEMAA
                     RAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRT
                     LKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDS
                     DLKVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNVTIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGAGASFQYSRSGKRDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVL
                     RHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVM
                     LLDAVDKNGDPVVSAVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKL
                     SRDNLLHRGLQLPKGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQM
                     QDKFQVLKAVQENIRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLA
                     DYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQA
                     QYDRLAGIIDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRT
                     GKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDK
                     DYWQVFTDDRPDTMRTIAERKDQETGETIREVVERPVPMAMMADRYFTTKKNGKTYYI
                     KLHDPRLMRAMKSMGPETSNAFVRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVM
                     NLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEF
                     VEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENA
                     LRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLV
                     RTLGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDK
                     VPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAG
                     NVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPF
                     GTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKFWIDYISGGTG
                     RFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEIAQYHAELK
                     SLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKS
                     VERDMKIAVDRFNREYNKKVGVD"
     gene            complement(10870..12135)
                     /locus_tag="ACHOTW_003237"
     CDS             complement(10870..12135)
                     /locus_tag="ACHOTW_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845287.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLARQQKNNERDFE
                     FAQSQFEHNKNVDQRNFDYRAKVDDRNYALKEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMIAHSQPLMEALGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGDKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            complement(12146..12397)
                     /locus_tag="ACHOTW_003238"
     CDS             complement(12146..12397)
                     /locus_tag="ACHOTW_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP9845288.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            complement(12407..12853)
                     /locus_tag="ACHOTW_003239"
     CDS             complement(12407..12853)
                     /locus_tag="ACHOTW_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845289.1"
                     /translation="MEYGKYETLARAGYSGADRPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            complement(12856..13512)
                     /locus_tag="ACHOTW_003240"
     CDS             complement(12856..13512)
                     /locus_tag="ACHOTW_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845290.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(13606..14007)
                     /locus_tag="ACHOTW_003241"
     CDS             complement(13606..14007)
                     /locus_tag="ACHOTW_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845291.1"
                     /translation="MAPVSGERMNDKILWYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTHLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            complement(14064..14204)
                     /locus_tag="ACHOTW_003242"
     CDS             complement(14064..14204)
                     /locus_tag="ACHOTW_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9845292.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(14437..15171)
                     /locus_tag="ACHOTW_003243"
     CDS             complement(14437..15171)
                     /locus_tag="ACHOTW_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9845293.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGEHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            complement(15262..15879)
                     /locus_tag="ACHOTW_003244"
     CDS             complement(15262..15879)
                     /locus_tag="ACHOTW_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845294.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            complement(15885..16163)
                     /locus_tag="ACHOTW_003245"
     CDS             complement(15885..16163)
                     /locus_tag="ACHOTW_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFP9845295.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINDNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(16178..17446)
                     /locus_tag="ACHOTW_003246"
     CDS             complement(16178..17446)
                     /locus_tag="ACHOTW_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9845296.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(17443..19068)
                     /locus_tag="ACHOTW_003247"
     CDS             complement(17443..19068)
                     /locus_tag="ACHOTW_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845297.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSKIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDANGNVALATEQIVSPEQWQSQFNPASIVAYPWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGA
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(19363..19551)
                     /locus_tag="ACHOTW_003248"
     CDS             complement(19363..19551)
                     /locus_tag="ACHOTW_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845298.1"
                     /translation="MPENRRIILDSKESWLIICDSQLGHLMRSMYQGRRFIQLNLEKL
                     KGVHDVALPVKWEFTRRQ"
BASE COUNT         4593 a         5594 c         4460 g         5046 t
ORIGIN      
        1 gagttgagct gggagcagca gagaacaacc ttattgatag tgaatgccat gttgctgaac
       61 tggaagaagc gctacgcgat aagcaggcgt tacttgaagc atcagagaag cgcatagcag
      121 aactggaagc cgaactcgta agccaaactt acaagttgcc acacacgcag tttgaacaga
      181 ttgctaacct ctacgaaatg caatttgatg acggtcgcac ttgtgccttt cacactgatg
      241 cgcaaaaggc tgagcaatgg cttcaggcgt gcgacggaaa cagggttcag gaatacgtta
      301 agctggaacg gttgcagaat gcgctgtctg gcaactctcc ggtaactccg gatggttgga
      361 taagttgtag tgagcgaatg ccggatacca aaacagccgt tcttgttgcc gtggagtttg
      421 acaggaaagg tgactggcga atgaagtggg cgacttacat cccggggcat cctgacgcta
      481 atgatgggtg gataattcct ggtgcgtcgt ggaaaccgtc acactggatg ccgctaccag
      541 aaccgccgca ggaggtgaac tgatggaaaa tgaaggcgat aatatcatca cactggtgca
      601 gccaaagcgc gatgaagaga agctgctgaa cattacagta actggcagaa aaaactacac
      661 acaacagagc tgtaagcatc gggccattga ggttcatgaa caggatcacg ttatcctgtg
      721 cttgcagtgc ggatgtgttg tggacccttt tcagtatgtt ctgcggtgtg cgaacgatgg
      781 tgaggctgtg gtgagagaga ttagacagct tcataacaga cacgatcagc tccgagaatc
      841 tgttgccagc ctcgagcgtg aagaaaagaa caccaaagca cggttgcggg cagcaagaac
      901 tgcaatactg tatgcggaaa atgaccttaa aaatattgag cagaaggtga atcaatgacc
      961 tggcctgaag cattcgcaaa tgtaggaaca gtaatggcgg tggtgctggt tgtgtattcg
     1021 atttgtcgct ggagataaaa acggtttgcg ggaaaaggag agttaagtag aattgctgcg
     1081 ggtgcttgag gctatctgcc tcgggcatga acaccaacgg cagatagaga aaagccccag
     1141 ttaacattac gcgtccggca agacgcttaa cattaatctg aggctcaatc catgctgaac
     1201 acatgtaggt tagcctctta cgtgccgaaa ggcaaggaga agcaggctat gaagcagcaa
     1261 aaggcgatgt taatcgccct gatcgtcatc tgtttaaccg tcatagtgac ggcactggta
     1321 acgaggaaag acctctgcga ggtgcgaatc cgaaccggtc agacggaggt cgctgtcttc
     1381 gtagactacg aatctgagaa gtaagagacc aagcggggga gtaatctccc gccacctctg
     1441 atgtgtcagg catcctcaac gcacccgcgc tttaccatac tgaaaatgct gtttgaatgt
     1501 tcatctctga aagaggacta tgaatgaaaa aggtattgat tgcagcactt atttctggtg
     1561 tgtcttttgg cgcttttgca cagcagggcg gtttccaagg gcctgaagca gagcgttcaa
     1621 cagtagcgca ggcaaaagaa ctgaaggatg atgcatgggt tatccttgaa gggagcatcg
     1681 ttaaaaaagt gggtgatgaa cgttatgagt ttcgtgacaa tagcgggaca attgtcacgg
     1741 atatcgatga cagcatatgg gccggtcaga atgtttctcc gaaagacaaa gtaagaattg
     1801 agggtgaaat tgataaagac ctgagcagtg ttgaagtgga tgtaaaggca ctgaaattat
     1861 taaagtaacc gtcccagctt gctaagcccg tcttactgac gggttttctg tttgttatca
     1921 cagtattttt caacaaaaca ccgaatctta tccggtgcgc tgtaaaaccc cgtccttcag
     1981 ggcgtggagg atgtcaaatg gcgtatattt ggatataaga aagccatcca atactggctg
     2041 aaaaaactcc tcatcgatat catcgtgggc ctgaatagtt ccatccgcaa ttctgattgc
     2101 cggtattgtt ccccggcgat aaggggtgaa catcatcccg gttacatcag atcgcctaat
     2161 aacatatcaa gattattttg ctcataaatg ctattcgcca ttaccgttgc tgcttcagct
     2221 taatgaaacg catagatccg ccatgctttt tcagcaaata cgctgccatt tgcgcaactt
     2281 tttcttcatt aaacataaat cggctctctg tatcacatga aatgctcttc tgactatatg
     2341 ttaatagctc cggttattac aggtcaatga gtgtttattt cttaatcagc cccgcacttc
     2401 cgtacggggc catttctgtt aatccactcc cacttttttg ttgtactcgc ggttaaaccg
     2461 atccacggca attttcatat cccgctctac cgatttcacc atcgccgact gttgcgccag
     2521 actgagagta ctgtcggcgt aaatggcatc acgctgttta cgcaaatcct tcagtctctt
     2581 ccgggtatcc tgcataagcc cgttcatcga caattttccg ttgttctcgt caatgaacgc
     2641 cgttctttct gcaccggtca gactcttcag ctctgcgtga tactgcgcaa tctctgtcat
     2701 ccggtcgtac atcttctgct ggtctgcata cggcatcacc tcccccgaaa ttttccccag
     2761 gaagggcacc tgctgttccg gtatatcaat accattcagc gatttcaccg ccgcatccgt
     2821 ggttttggaa atgaagcgcc ctgtccctcc ggagatatag tcaatccaga atttcagcga
     2881 ttccggtgtg atatccaccg cgccgggacg gtactggctg ccacctgaga acgcattcag
     2941 ccaggatgca aacgccttgt acgcttctgg cgttgaacgt cttcccagct ggctgtcagg
     3001 ttttggtgta ccaaacggca tgttctcctg gtaaatctgc gcccccatga agttttcatt
     3061 catggcaagg ttcgcaaacg gacgcagaat ggtcggcgct gcatttttca gcaatgcccc
     3121 ggacagtgtt tccgacgtct cactgccaat cgggctgaat gcaccaagca caccaccaac
     3181 aacattaccg gcagcacggg acgccgtcag gtcacccgcc gccacacctt cagcagtatg
     3241 cccgagcagg aagaaaacgt tgtacccgta aggcagagga atactccagt actctccggc
     3301 cttgcccccg aacaccgatt tcataatgac gaggttacgc tctttcacat gagacggcac
     3361 cttgtcatac cagttaaccc cgtcatcatc ctcacccgcc acgctgcggt taagcgagcc
     3421 aagcagataa cctgctccca cagcggcaag cgcgattttc tgcggaacat tcagattctt
     3481 ccagcgaagg cgctccagta aaggtccctc accattaaga tgtccgagcg ttctcaccag
     3541 atttgccgtt ccctgaatgc tggcgttggc gaacatgtac agcgaattca tcagcgcccc
     3601 ctgctcacca cgacggttaa agttcaccgt catgtttttg gcaagagacg ccgcctgctg
     3661 gcgtgacaaa ccggcatcac gggcgtgctt ataggcagaa agacgcagag cgttttcaac
     3721 cgcaccgttg gcatcctcga caaggttaag gaacgaattc catgcaccga tactctggcc
     3781 tttccatccc cccttcgcca gtgagacaag gcgatccatt tccttctgct ggccttcaag
     3841 gtcacccatg ttaaaccagc cggttttccc tccgtcctca acaaactctt tccacacctt
     3901 ctgccactgt gcaccttttc ccgtgagggt tttaccacgc agactggcgt atacggctga
     3961 catggcagaa cggctgtctt tcaccacagc cagggcggat aagttatcca gccctttcag
     4021 tttaccgtcg ctccttccct gctccgcctt caggttcatc accgccgtct gcacgtcacg
     4081 gatgaagtta ctgaccagga attccgggtt atacgacgtg ttcaccgttg ccaggaagcg
     4141 gttaactttc cccagcgtac gaacaaaggc attgctggtt tccggtccca tactcttcat
     4201 cgcacgcatc aggcgcggat catggagttt gatgtagtac gttttgccgt tctttttggt
     4261 ggtgaagtac cggtctgcca tcattgccat cggtacaggg cgttcgacaa cttcgcgaat
     4321 ggtttcacca gtttcctggt ccttgcgctc tgcaatcgtc cgcatggtat ccggtctgtc
     4381 atcggtgaat acctgccagt aatccttgtc gggattatcc tgcaccagtt tcaggaaggc
     4441 gttacccact tcgtttttgc gatggcggat cagcgattcg ctcaggtcct gtatcgcctg
     4501 agtggacgga gactgtgccc gggatgcacg ccccatggcc tgcctgcttt cacgtccgcc
     4561 gatggtgaag cccttacctg tacggggcag tgacaccaca ccgtcaacat cctgaccttt
     4621 caggggaacg tagtaacggt aggcgttctg ccaggcatcc accacaccgt tctcttcaag
     4681 tccggcctca cggataagct cacggcgacg ggccagcata tcgtcaataa tccctgccag
     4741 acggtcatac tgtgcctgtt tgccactgtt acgtacacgc tgcatgattt ccgccgcttc
     4801 cgcgttggtc atccccgaac cgccgtccgg cattttcggg ttgattttcg cgatatgcgc
     4861 gttacgttcc ggcgcgtgac gggcgtagag gtactcatcc agatcggcct gcgcaatttt
     4921 gtagtccgcc agtaatttag ccagtggctg aacgtagcgc tccttcatca cgttcaggtc
     4981 gttttccgcc ttcccgtgga agagttcttc cgccatataa gcgttgttac tgtcgtccac
     5041 tttgccgcca gttttacgga tattctcctg aacagctttc agcacctgga atttatcctg
     5101 catctggcgc acaaaacgcg atgcaattgt ctcttccggt gtcagactgc tggtacggga
     5161 gtaatacggc cccttgcgaa tatcttcagg atagagtatt ttatccgcag agccgcgata
     5221 agaatgttcc cctttgggca attggagccc tctgtgcaga aggttatcgc ggcttaattt
     5281 ttctctccgg tacaacgtta acccggtcat ttccatactc ttcagttttt ttccattttc
     5341 tgtaccgtaa acagaagcta ccttgttgat ttccagacgc gaccggacag ccttcatgtg
     5401 taccgctgac accaccggat caccattttt atccacggca tcaagcagca tcacaaccgc
     5461 atttctttct gtcgctgagc ggtaaattgc atccggatcg tgcatcagtt ccggtagtct
     5521 ctcgataaca tccatcggca ccacatgttt cacaccattg gtggccttcc gcacagtatc
     5581 gcgggaaata accagcggca aatccggtgc accaaggtga cgcaataccg gcggcgtacg
     5641 cccgatgttt accgttaaat ctgtggtgcg cagagatttc atcattctgg caaggtcatc
     5701 acgataacgc tcgccctcac cttccggcac tttgaacgga tcacgtttac cactacggga
     5761 gtactgaaac gatgcgcccg cgccatcctc acgcggcgtg taaccttccc gcacacgctg
     5821 gcctaacgta cggatggtct cgcgaacaag tctgatatcg ttcagttccg tcggcttcag
     5881 taaccccgta cgacgcagta cccctttgac cagggcaaca acacgctccc atgccgccac
     5941 gaatttattc ggctgtttct ccgccatatg tgccagaaat tcacccgcct gtacttccgg
     6001 tgattcctta ccataggaag catcaacctt acgccaggct tcacggatgg tgacgttatc
     6061 actgtcgcgg gttttcagca cggttttgat aatcgtctga tattccgctg gtgtgacaac
     6121 atgctccatg gcatggtgaa tgatctcgtg acgcagcttc tcgcgaacgg tccgcccgtc
     6181 agggatgtta tccgccacca ggacaatttc tcgtttatcc ggacgataga atgcgtgcac
     6241 cctgccgtaa ccatcgaaca attcacccgc cagcgcttca gcctctttct gtgacttcac
     6301 cacacggacc ttcaggtcac tgtccttaat gccgctcatc acgccacggg caaccgcttc
     6361 aacctgcggg accgggctgc ctttggcttc cgcactacgg ttaacatccg aaatgagatt
     6421 accttcaggt gtgcgggtaa cgcccttacg ggaataaaac gcaacgccct tgtccgtctc
     6481 acgggttttc agggtgcgga acaggtgatc gaatgcctca cgaataccgc catccagttc
     6541 cgcattcgtc ggataagcgt aggtgttatc tgtgttgtgc tcaggtgcct tacggatatt
     6601 gaccagataa tcattctcca cgccagccat acgcgcctta tcctgaacat aacgctcaaa
     6661 ggcacgtgcc gccatttcaa catccgttga ccagtacggt tttgagcgca ccttatcgag
     6721 aagcgctgaa cgacgcggca tgtcactgtt tttaatggcc tgaatcacac ctttaaaagc
     6781 gtcgtaaacc tcctgacgta ccggatattc agcatcaaca tacctgccgt ctttaaatat
     6841 gcgcctgaca cgctgtgctt ccgtcataaa gtcgccacct gacgtaattt tcccgtcatt
     6901 ggaaacgtca taacgaccaa aataattatc cagagaatgg aaccattcgt gcgccagcgc
     6961 acccggtccg ttaccttttg tcaggttgat tgccacctca cctgactcat agtgtgccgc
     7021 cgccttaccc ttaccacggg caccaaatgc caggccaaga cgaccgttca gggaaagcgc
     7081 ttttgtcggt acattcagta cttccgcaag gtcatgcagc gagtcataag cccggttcaa
     7141 atcagcctga cgacgcggac cttccacgta gttaccaaac tgcacaccac ggaaaccaaa
     7201 cgcatcactg aactgctccg gtgaaacatt ccccttgcgg cgttctggtc cggtacggtc
     7261 gcggttggtg gcgttgcgct gctcctcacg cgaaatctcc cgcatctcct tcacatgacg
     7321 aacaagctca tcacgatgtg aatcaatgta cttacgcgca tcactggctg acttaaagcc
     7381 acctctcacc cgcattttgt ttttaccgta agcgataaaa atatcgccac tgcgggtatt
     7441 ccggtaaacg tcaaagcgga ttttgtcatc cggtgacggt gctgtttttt catcaccttt
     7501 cgcctgtgat ttttcctcct gctctgcaaa ccagactttc gcctttgcca gtaattcatc
     7561 cctgctttcc gagaaaaaga ggttactccc cttattgtcc ttattgcgca gtgaataaag
     7621 tttctgtggc ggatcgtaac gcttccctcc tgccgcctga tacacacccg ataccacccg
     7681 ataagcagaa gccctgtcca tctgtgaggg tggcagagtg cgtaacagtt gccaggtatc
     7741 cgcgtaacgg gagggcattc tgccttccat ccattctgcg aggcgtttcg cgctgaccgt
     7801 tccgttcagc atttccgata cactgtgtcg tacttttttt atgctctccc cccagcctgc
     7861 cgtattgtgt ttcgtcttcg ccggaatatc gctacgatac agcgctatca ttgccagggt
     7921 atcagcatca gcaccttcgt tcgccagttt tgcgtagtcc ggtttcggga acagtttgct
     7981 cagcggctgc gtggcataat ccctgtcttc cagcgtttta cccagtgttt cagcaagctg
     8041 tgcataacgg tgttttgccg cgcccttaat ttcttcacca aagtcttcaa tttttttgcc
     8101 cctgacttca cctccgcgca cggtagcttc cctgtcagta gagacagttg tttcagattc
     8161 aggtgcgatt gtctcaggct gcgggatgtc acgatttcct ccggcctgtc ggcgtacttc
     8221 tgcctgctgt gcaagctcag gcagtgagtc ccgtacagtg cggggaagtg attcaggctg
     8281 aggcatacga acattacgcg caatgtcagg agccggtagc ccttcacgta ccggtgctgt
     8341 cgcgttctgc gcatccggtg acggcagacc acgacgaacc atgtcaccct cgagtgtttc
     8401 cccctggcga ccagcggcgt tttccggtgc cggagcttgt cctttctgga aactctggcc
     8461 cttaacctca ccggttgtgg taaaacgacc accacgtccg gcctgatttt catccggcgt
     8521 acgcgccact tctcccggta acggatatcc ctgtccggga tgaatatcgc cgggagcggg
     8581 caggcgtgga cgctcagtta actcctgtgc tgtcggaccg gcatcacctt cagccatctg
     8641 tgaacgtaca agctcctctg ccgtcggcgc gttgccacgg gcaaggcggg cctgtacctc
     8701 cgtatcatcc cccgtgaaac cacggaatcg cgggtcacgc atgaacgcgg gctgctccat
     8761 cgggtcagca tcagcaatac gctctccgtc agccagggtg ttaatggttt ccgttgccac
     8821 atcttcagag aacgcaggat tatccatttc aacggcatac tgattctcgc ctttcttcac
     8881 tacggacggt ttcaggccgg tggcggcggc attacggaaa acatcactcc ccatggcgct
     8941 tttctcatcc gtgaaataac ggttgtccgg gcgcaccttc tcaatgcgtt ccgctggtct
     9001 ggacgcacca ttcccgttaa tttcaatgcg gacttcattc gggtcccgga accgcaccgt
     9061 cgggtaaatg ctcccgttac catcctccgc attttcaggc tgtgactgtg cttccggcgt
     9121 cacttcctgt tgtgggcgaa tgacttctcg tattgcctga aactgttgtt cttcagtctc
     9181 ggtgcgcccc tctttctggc tgagtctgcg gtattcctca agcagttcag aacgcggctt
     9241 cgccttcagc tcattcatca cagcctgtcg cttcgtctgt tcgtccagtt cattcaacaa
     9301 ctggctggca gcttcccggc gatgagctgc ggatgcgtca ccctctgttg ccatatccgc
     9361 atcagcatac tgctccagaa gctgctcgcg attcatgccc tgcatggatt cacgctgctg
     9421 cgccaccgga tcaacagatt ctggttgcgg ggctgcgtcg tcctgctgca cggtttcagc
     9481 atcacgcatg gcggtttctt ctgcggcctg acgtctgcca cgatatccgg caaccgcacc
     9541 gaatggagcc cccatcgcag caccaaaggc tgcaccttcg atcgttgcgt cagccacgcc
     9601 ctcccacggt gacacaccca ttccggcggt ctcacgcaat gccgtgtttt cctgatagcg
     9661 tgaatagccg ccctgcgccg cattaatcgc cccctgttcc gtggcatttc tgacaatgcc
     9721 gcttttaacg gtcttcgctg tgcctcgtgt caccagatta aacagttgtg cgtcacccag
     9781 ttttgccgcc atggcattca cagccagcaa ttcaggatcg gttgccagct gcgcgcgcac
     9841 ctcatcggca acacgctctt ttgccagatc cattttctgg cgatcagtaa gctgtgcgtg
     9901 ctgcgggtcg gcgtcaatgg acaaaaacgt ctgctgaaat ttcggtgact gcgccagctc
     9961 agagtaatca gcattaagaa cagcatctgc tgccgccatt gcactctgcc cctgtgcact
    10021 tacagtggaa tgggtgatca ggcccgcctg gaataaatcc ggcatttttt tatcgacagc
    10081 ttctgctgcc agtgccgtgg ctctttccgg ctgcatccct gccgcgatgt attttttctc
    10141 cagcccggcg gtcagcattt ttcgcagtgt gacatcaccc acctttctag ccacaccgcc
    10201 agcaaccata tcaggtacaa gcgcaccaat caggtttaca cccttcgcca cccagaccgc
    10261 agaatcatta tagccttcgg tcatcggcgt attcagcgca cgaactgcac ccggcgacat
    10321 cttaccggtc agccattcat cagtggcttt ggcaccatca ctgacagcct taccggttgc
    10381 cttcagacct ttaccgacaa tatcagtaac cgcgtttttg ccatcaggca gggtatcgat
    10441 gacctcatca gcccccctgc tgccaccggc aaaaatatcc tgcacagttg cgacacccgg
    10501 cagccccata cggctgaact catttaaaat acgcgcccct gtttttaccg ggctctgaat
    10561 catcgcatca ccgagtccac gggccatttc ccctgttccc cggacggact gggcgaaacc
    10621 tttacccatt gttggcaata catcgcccag gctgaacgac gtactgttat ccttccagcg
    10681 attcggatca gagaaaaatg cttcatagct gtcagtttcg ccgggttgct gaatgttcag
    10741 gctgttacga ttctggttac cgagttgcgc ctcaggacgc tgttcctctg aataggccat
    10801 acagactcca taaaaaaacc cggcacaatg gccgggcatc aggaaatggt attaatggaa
    10861 tatcgtgaat tactgggcgt aattctgttt cagtccacgg ataaactgag atgcagatga
    10921 agcactttca tctctggcct gttcccccct ggccttttga atacggagaa aattcgcata
    10981 cccgttctcc agcaatttct gattctgagg ttccagcatt tcaggctgac gggccgctac
    11041 atttctggca aatgacagct tgtcaggatc atctcccgcc cagttgataa cctgttgctg
    11101 aagtttttgt tgctgaattt tctgttgatg cggttgtgaa ccagtcgcag cataatattc
    11161 atccaccgca gcctgagcat taccgccatt cttgatcgcg tccgccgcta cgttgctggc
    11221 ccccttctga agctctttca gagaaagtcc ctgctgcttc ggcataaaat aaccataatt
    11281 tttcgatatc tctgcaaatt tgctgcgatc cctgacctga gcgatagcct tatcaacggg
    11341 tatcgccagc acggtttgat catcagggtg cgcactgccg tattctgtta caggtttatg
    11401 cgcggtggag ccatcgctgt atgtgagatc aaggccaata agtacgtacc cttcctgctg
    11461 cgctggtacg atactgccaa tcctggcctc ttttatcgtt ttgtccccgg ttgaatcagg
    11521 catgccaata cgctgttgta gttccggggc aaacacgccg gaaagcacat cgagattttc
    11581 cggggtattc agcgaatcga tcgccatgtc cggcttatca ccaaagattt tttgcaggtt
    11641 gatcacggcc tgacccgctt tcgctgcata gccttcgttt gacataagaa ttaatggatg
    11701 tccctttggc agctgcccga acagacgcgt tgcagcctcc tgatcgccag cctcaattgc
    11761 ttttccaagc gcttccatta gtggctggct atgggcaatc atatcgttat actcaagccg
    11821 tcgctggttg tatttctgca actggagtcg ctgctgctcc atacccagtg acgcattccg
    11881 gtaattctgg ttagcgttaa actccctctc cttcagtgca taattacggt cgtcaacttt
    11941 ggctctgtaa tcaaagttcc gctgatcaac gtttttatta tgttcaaact gagactgcgc
    12001 aaactcaaaa tcgcgctcgt tatttttctg ttgccgggca agctgtgctt cacgcagacc
    12061 aagctcctta cgacgggtca ttgcctggtc aacggtgctg aatccggcaa gtaacccctg
    12121 tgcaaatccg ctcattcacc actccttaaa acaaagaacc agcaatgccg ccaattactg
    12181 ctccagcaac agcaccaaca ggaccaccaa cggatgcgcc aatagccgcc cccgtaccaa
    12241 tgcccgtacc gatattctgc ttgttctgcg ctttctgttg cgccgccatc tgtttgttcg
    12301 cagcctcaat ttcttcacgt cgtctgtctg cgtcacttat tccctgtaat gcctcacgcc
    12361 gcgactgatt tgcaatatcc agtaaaccgt atcccatatt gcccccttac gctgccacca
    12421 gttggccgcc aacactcagt ttctgtcttg ccggtgcaga agcccccgtc agaatattca
    12481 tctggcgatc ctgttcggct tcacggatac catttttcgc gccagcaatt gccagggcat
    12541 tacgtaaccc cagcgtatta ctgtcgggat tatccgggcg gtttaccccg tatctcgcca
    12601 tctggttatc ctgcgccatc tgcgctgtac ggagactgga agtggcaagg cctcccaccc
    12661 gtgcaagctg tgcattcatc agactgttgt tctccccaag gtcagccagc cttgccacgc
    12721 ggggcaaata tctggttcgc cagtcgtcgt attgttggcg tgtcagcgct gctgacgtct
    12781 gccagtcacc ctgtgggcgg tctgctcctg aataaccagc ccttgcgaga gtttcgtatt
    12841 tgccatactc cataattaca gtctccagtt ctgagcctga tgctgaatgg cattagcgcc
    12901 ggtgccaggt gttttagcac cgccacttcc tccgctaccg ccagccttat gcatcgcata
    12961 tgcacctgcc gcacccagac cagcgccaac aagagaggct cgcccctgct gtttcgtaaa
    13021 cgccgcctgt gcatccgatt tagcttttgc cagactgctg tctgccagag agttaaaact
    13081 ctgtaacgca tccgccttct gaccagaacc gagagcagca acatcctgta gcccggcaac
    13141 atacttatct gcctgcgata cctgcccccg tgtggttgtg tcaatctgcc cggttacctg
    13201 gtcactctgg ttcgcattca ttaccgcatt aaaacgacca ctggatgggt caacgccgga
    13261 ctgagcaaga ttacccgcaa gctccttccg tgcttcgcca aactgtttct gataccccag
    13321 atttgtggtg ccagcaatat tgtcgtactg ctgctcactg ttcaggtcat cgaccttttc
    13381 catgaagtta tcttcagccg ggcggaggat atttttgtaa tcctgccacc ctttccaggc
    13441 cacttcttcc tgtgctattt gcgctgctgt tggttttact ttggtatcac caccgccttt
    13501 acttccaccc ataatggccc cctggataac aaaaaaccct gccggagcag ggtcagaatg
    13561 tgaattacaa tgttggttta aacacaatga tgaaaactgt agcagtcaga ccgctatcct
    13621 gaacaccatg aagccatcct catcatccgg cattcgctca aagcccagtc gttttcccag
    13681 ccggataaat ccccgccttg ccgtatggaa ctcagcccag cgtccgccag ccagatgggt
    13741 taatgtcttc acctccggca ggtaacgctc aacgctgtta ctccccgtac acacgcccag
    13801 caacaccaga acataaggga taccatcagc actgagcaca gaacgcagca ccaaaaagcc
    13861 atcaggtgcc tcaaaacaaa acgcctgctt tttaaggcag gcgtctttaa cttcattcat
    13921 aaattcaggg ttgcgggaat ttctcacaac acgctgcata taccagagaa ttttatcgtt
    13981 cattctctca cctgaaacgg gtgccatatc ggctgaacct cagcaaccag ttgacgggga
    14041 ctctcgtccc cgtcgcggtt ttcctactgc ttacactgta agaacgccgc aaactccgct
    14101 ccccacaaat tcagccggaa ctcacacagt gaaccgtgca acatccagat ggtgaagata
    14161 accgtcatgc aaattgtgac ggtgataagc gatttttgcg acatagcgct tgactccttt
    14221 tacagagagg cgctaacctt ctacttgctt aaggtacgaa gtgttagggc ctcgggttaa
    14281 cgttaagttg actcggggcc tttccacatc aggccttcag gttcaccctc cagccatcag
    14341 ccgaaaggca cccacgcata atttacggtt tttgccctgt acgggcaata aaaaacccgc
    14401 catcacagcg ggtagtaaac ggttaccgga tacaggtcag aatttcaggc caatgccagc
    14461 agtaacgcca ctcgtgcgcc agtcgccaga gccggaacct tcgtaagcca catccagtgt
    14521 aacactctca ttcaggttaa actgtgcacc tgcagcccac gcaagggagg tttttttcgt
    14581 gctgttgctt tcagaaaatc cgccactact gttaatattg tccttaattt tcaggtcagc
    14641 gctaacttta gcaacgccca tccctgccat cgcatataaa ctcatgtact gattaacccg
    14701 ccatgaaggc cccgctaaca gactccagta attcgccctg atatccgttc tggcagaagc
    14761 tgccggattc ttaatcttcc tggtatgatc ggctgactgc acatcaataa atgtctgtga
    14821 gttagtgaga gaacgcgtcc atgtaaaaga ggtgataacg ccaaaatcat ccgttatctc
    14881 gtagcgatac ctgatattaa tgccctgtgg atttttatcc ttgccttcat atccactgac
    14941 ccgaccatct gtatattcgc ccaatgaaga aaagtagttt ctgttgacga aatgactgaa
    15001 agtctccctg ttatgagcag tcgcatcctt tacaaaatcc ttcagtcccg gaaactgaaa
    15061 gtgcgcatac cccagagaaa ttgtatgctc tccttctgct gcatgtgcag ataaatttac
    15121 acaggcaatc ccggagattg cacacacaac cagtgttgct atactcttca tttaagatat
    15181 cctgtattca gataacacgc taaacttttt aataatacat ctttttagac attttaaatg
    15241 aatattaaag actcgcttca actaatcata tactgatgta ttaataacca ggaccttatc
    15301 tatatacggc tttcgaatat cccatgttgc accaccggaa taatgctcac aggaatatat
    15361 tttatttcct gtagcaccag tggatgtggt ataaatcggt cgatcataag gagttctttt
    15421 ccagttataa tacccgacca gtgcaggcat gattgcgcat ggatatccaa ggtccttttc
    15481 aaatttgata tcaacaggta ttaacttcgc gtcaagcagc atcatttcgc catgataaat
    15541 catctcaccg tccgggttat acatggcgat accatactca gaaggtggag taaccatatt
    15601 cgcgaaagca taaaccgtcg taacaccagg gttcgttccc ctgacaatta catgaagcct
    15661 tagtgcatga tatccatcaa tctgttcatg cgtgtacatg acatcggcct tcttctctgt
    15721 tctgataaag aaaaaacaac ttttgcctga cgggattgat gttttaaaaa aagacttttc
    15781 agtcgccggt atggtgcctt tgttgatcag acactgcggc gtaaaacctg gacttatcca
    15841 tacgctgcca tccggcttca taatgctcaa accgtacata acacttatcc ccagaatgta
    15901 taaatataag atcccatacc ctgctcaaga ttcgaccacg tcaccgtatt gtcattaatg
    15961 gttatcttcg gtactttccg atccgcaaat acattattcc agggaaataa acaacataca
    16021 gcctgcaatg atttcccgtc gggtttatta gtgtacgtct ttgagccaga ctccgctgta
    16081 aatctgtcca ggaaaaatac tggagtcagc acgcccgtaa cattaacgtt atttctgtta
    16141 taaatggcaa aaccgtattc caatactcac ctcctgatca gcgtaatctg cctatgcgaa
    16201 cagccagtcg tccattctgg tcataaacct caattttatc attgcggatc accagtccta
    16261 cattctgatt agagtaacga attgtcagtt gcccttgtga cgtaacactg aaaaggcctc
    16321 caatattcag gttaccctga gaatcaacct gaaagtttcc gttctgaata acggcactcc
    16381 ggataactgg cgaagtgata cttaccccgg cttttacctc atccgccaca accttccgcg
    16441 acaccagtgt ttcaatcacc gcgtcataaa tcatcgcttt cgggatcact accttgccac
    16501 ctgataccgc aaacggatag gctgtgttat ccggattatt cgggtcaaag acaaacaact
    16561 gcgacgcaga aattgcaacc tgacttacag gcctgccttc actgtctttt ccggcgacaa
    16621 tcccgatccc cgcagtgata ccatcaactc ccgctttttt tgaccacatt gcaagaaaag
    16681 cttcaccgcc ttctttatcc aatttagtga tgcgcttgtc gacctcatta agcgattcac
    16741 tggttgacga atccagtgtg ctaatgcggg tttcaatacc accaatcgtt cttgtcgttt
    16801 cttccctgag agtccctaca acttcggttg tcttaattgc agcatccttt acagcctgcc
    16861 cctgcgcgtt ttttatttct ttacgcagct cggacacaac cggcgacttt gcagcctcat
    16921 cgcggatctg gtcaatgatg gccttcacgc cgatctgtgt ttgtgcctga gtgccttttt
    16981 cagcattcca tggacctttc actcctgccg cgttaacaaa acgtatccag taaaatcccg
    17041 accagccagg gtcaaccgga tcgccgtaaa cctgccccgg cgtcgtggca accagcactg
    17101 catcagcaag gtcatcctcc gtaccccgcc agatttcagt cagtgaatgt ccgcgataat
    17161 taggcatatc ccattcaaga agaaccgagc caaatccacc ggtcgcctta aaattcagcg
    17221 gttttgtggg aaaatcaaca gtcattaaag tactgtcaat ctcaataccc ggattcagtg
    17281 catatgaggc accacccgat gttcgacgcc gggcgagttt aagaccaacc agttcctcac
    17341 gggtcacaaa tgcgtgacgt ccgtcaccac gctgcccggt gccaatttcc atattctcca
    17401 caacagtgga taaatccttc cccgcacgcc acggttttct ggtcataccg gcatctccga
    17461 cattgatgta ctcagggtta ttcgttccac ctgcccgaat ccggatacca tcacctgcca
    17521 gttttgcccg gttgctgccg gaagtctcac cacacttccc ttaaacgtac ccggcgcaaa
    17581 atgaatcaca ggaacatcat cagccataat ggtgatcccc acacgctcag gcgccggaga
    17641 tttcaccctg atacaggaaa aagaggttct ttcaggtaat gaaaaaattt ttgaatgcca
    17701 ccttatcgtg gagggcagag cccccccggc aagcactgac attttgtctc ctgtcaccac
    17761 gcgcatcata tctttcgcga gatcaaccca tgcgcagtca aacggtgtac tgagataacg
    17821 gatatccatg ttcaccggac tgaatacaaa cacatcctgc ttaccatccg gtttcgtgta
    17881 acaggcaatg tattcaccac gccagggata agccacaatg gaggccggat taaactgact
    17941 ctgccactgt tccggtgaaa caatctgttc cgtcgccagc gcgacattac cgtttgcatc
    18001 aacagacacc aggccatttg ttcctgcata cagcacaaaa ccctccatcg caaccatact
    18061 ccgcctgctc agacacgcct gcattgaagg gattttggaa ccagaaattg tggacggtga
    18121 taccccactg aacaaataag gctccccctt tgtcgccacc accagtgacg ttcccagcgg
    18181 acagatagct acaatatctt ctgccgtcgt gtgacgattc acttccggcc atgcatacgg
    18241 cagatacgct tccgaaaaca tcacttcatt accggcaaac ccggcggcaa taccgttagc
    18301 catcaggcaa aggcctgtca tattctctgg cggcggcagg taatcccatg tcgccaggga
    18361 aggcccaagg tttttcgccg gtattttgtc cgtgtaactg agcacggatg catccagttc
    18421 agccacaagt aaaaaatccg cctcccctcc acctgatgca gagcgataaa tccggcgacg
    18481 tttaatactg gcattctgca atggcaccgg agccagcgtc agttgtaccg cagttcccgg
    18541 agtacggagt gttacctcca gagacgccgg acctggcgga ccttcttcac cataatctga
    18601 gacaaaggtt tccgtataaa accgggtttc atcatcattc gggttatcgt cagaaacatc
    18661 accgccctgc tgaacagtac agacaggagc tgtcgtcggc gcggggatcc ccagacgata
    18721 cgatgatgtc gggtgattcc cgtccccttt tgtggcaata gtcgcatccg tcactttagg
    18781 aaaacgcccg tcagtgtagt aaatacgccc gtgggggtcc tgagcgatcg gactgcggat
    18841 cacatccacc acatccggcc atgcaaacca gaaatcgtca cggtaatgaa aaattgtttt
    18901 tggcttaatt gtgaatgttt tctcaacccc ggatatctga cgttctggtg taataacacc
    18961 aaaccggaaa tggcagtcct ccgccagtac agcggaatgc tcgggcagca tggatgtcac
    19021 aacgcgcggc atcatcccac gcatcgtggt gatatcgata tagggcatag aaattccttt
    19081 gactgcttca ggcttaaaaa tcaggggatt tatgatggaa ttcgctaaaa tcgcgatcgg
    19141 catcactgaa acaaaatgcc ttgcatgaca ttctgatgat gactaaataa cttcattgat
    19201 tattgcaatt ttctttcggt ttgggggagc atattgctcc ccttttttca ctgcattatc
    19261 attgccgctg tagcaacgag aagcatcact ccgcacacgc aagatgtttc acactcacac
    19321 catacagtcc atttttcgcc attcccgtat acagaaagct attcactgtc ttcgtgtgaa
    19381 ttcccatttc actggcaagg cgacatcatg tacccctttc aatttttcca gattcagctg
    19441 aataaaacgg cgtccctgat acatactgcg cattaaatgg cccaactgac tatcacagat
    19501 tattaaccag gattctttac tatccagaat aatcctgcga ttttccggca taaaaaactc
    19561 ttgaaccaga attacacggc atccttcact ccatcgtatt aacatgttta ttacttcact
    19621 ctctaataac ccccccctat acatgacaac aaaaaccgga gccgggctcc ggtttttgtg
    19681 aagctgtcgg cta
//
LOCUS       DAZKIF010000093        19539 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZKIF010000093 DAZKIF010000000
VERSION     DAZKIF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.093"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..353)
                     /gene="yhaI"
                     /locus_tag="ACHOTW_003249"
     CDS             complement(<1..353)
                     /gene="yhaI"
                     /locus_tag="ACHOTW_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9845299.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGS"
     gene            complement(594..959)
                     /gene="yhaH"
                     /locus_tag="ACHOTW_003250"
     CDS             complement(594..959)
                     /gene="yhaH"
                     /locus_tag="ACHOTW_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9845300.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(1251..2237)
                     /gene="yqjG"
                     /locus_tag="ACHOTW_003251"
     CDS             complement(1251..2237)
                     /gene="yqjG"
                     /locus_tag="ACHOTW_003251"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP9845301.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDARLG"
     gene            complement(2307..2699)
                     /gene="yqjF"
                     /locus_tag="ACHOTW_003252"
     CDS             complement(2307..2699)
                     /gene="yqjF"
                     /locus_tag="ACHOTW_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9845302.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2885..3184)
                     /gene="yqjK"
                     /locus_tag="ACHOTW_003253"
     CDS             complement(2885..3184)
                     /gene="yqjK"
                     /locus_tag="ACHOTW_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP9845303.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(3174..3578)
                     /gene="yqjE"
                     /locus_tag="ACHOTW_003254"
     CDS             complement(3174..3578)
                     /gene="yqjE"
                     /locus_tag="ACHOTW_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9845304.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3581..3886)
                     /gene="yqjD"
                     /locus_tag="ACHOTW_003255"
     CDS             complement(3581..3886)
                     /gene="yqjD"
                     /locus_tag="ACHOTW_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP9845305.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3924..4292)
                     /gene="yqjC"
                     /locus_tag="ACHOTW_003256"
     CDS             complement(3924..4292)
                     /gene="yqjC"
                     /locus_tag="ACHOTW_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP9845306.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(4439..4822)
                     /gene="mzrA"
                     /locus_tag="ACHOTW_003257"
     CDS             complement(4439..4822)
                     /gene="mzrA"
                     /locus_tag="ACHOTW_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP9845307.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4826..5488)
                     /gene="yqjA"
                     /locus_tag="ACHOTW_003258"
     CDS             complement(4826..5488)
                     /gene="yqjA"
                     /locus_tag="ACHOTW_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP9845308.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5510..5593)
                     /gene="yqiM"
                     /locus_tag="ACHOTW_003259"
     CDS             complement(5510..5593)
                     /gene="yqiM"
                     /locus_tag="ACHOTW_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFP9845309.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5833..6609)
                     /gene="exuR"
                     /locus_tag="ACHOTW_003260"
     CDS             complement(5833..6609)
                     /gene="exuR"
                     /locus_tag="ACHOTW_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP9845310.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6739..8037)
                     /gene="exuT"
                     /locus_tag="ACHOTW_003261"
     CDS             complement(6739..8037)
                     /gene="exuT"
                     /locus_tag="ACHOTW_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP9845311.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            8520..9932
                     /gene="uxaC"
                     /locus_tag="ACHOTW_003262"
     CDS             8520..9932
                     /gene="uxaC"
                     /locus_tag="ACHOTW_003262"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9845312.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9947..11434
                     /gene="uxaA"
                     /locus_tag="ACHOTW_003263"
     CDS             9947..11434
                     /gene="uxaA"
                     /locus_tag="ACHOTW_003263"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP9845313.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            11517..12068
                     /gene="ygjV"
                     /locus_tag="ACHOTW_003264"
     CDS             11517..12068
                     /gene="ygjV"
                     /locus_tag="ACHOTW_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP9845314.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(12073..13317)
                     /gene="sstT"
                     /locus_tag="ACHOTW_003265"
     CDS             complement(12073..13317)
                     /gene="sstT"
                     /locus_tag="ACHOTW_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9845315.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13716..14681)
                     /gene="alx"
                     /locus_tag="ACHOTW_003266"
     CDS             complement(13716..14681)
                     /gene="alx"
                     /locus_tag="ACHOTW_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP9845316.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(14965..15951)
                     /gene="ygjR"
                     /locus_tag="ACHOTW_003267"
     CDS             complement(14965..15951)
                     /gene="ygjR"
                     /locus_tag="ACHOTW_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9845317.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(16030..16722)
                     /gene="ygjQ"
                     /locus_tag="ACHOTW_003268"
     CDS             complement(16030..16722)
                     /gene="ygjQ"
                     /locus_tag="ACHOTW_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP9845318.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(16799..17302)
                     /gene="ygjP"
                     /locus_tag="ACHOTW_003269"
     CDS             complement(16799..17302)
                     /gene="ygjP"
                     /locus_tag="ACHOTW_003269"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP9845319.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17387..18523
                     /gene="rlmG"
                     /locus_tag="ACHOTW_003270"
     CDS             17387..18523
                     /gene="rlmG"
                     /locus_tag="ACHOTW_003270"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP9845320.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18807..19121
                     /gene="higB"
                     /locus_tag="ACHOTW_003271"
     CDS             18807..19121
                     /gene="higB"
                     /locus_tag="ACHOTW_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFP9845321.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            19118..19534
                     /gene="higA"
                     /locus_tag="ACHOTW_003272"
     CDS             19118..19534
                     /gene="higA"
                     /locus_tag="ACHOTW_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFP9845322.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         5057 a         5013 c         4925 g         4544 t
ORIGIN      
        1 tttgaaccaa acttcggatc gtttccgtaa cgattgctgc cagaagtacc ttctgtgcag
       61 aaaaaaacca gaagcacgag ccagccaata aacggaacaa aaaagagcaa ggcccaggca
      121 cctgaacggt cggtatcgtg taagcgacgg atagcaagcg caaggacggg aaggaaggtt
      181 gccagcaaat aaagcataga cagatatgga agctccagac ctaaaatcaa ttgaatgaca
      241 ttgataatag cgccgacgat ggcgttaatc agagtaaaca tccagtactc tttacgacgt
      301 gcgcgaccag agaaaccaac ataatttttt aatacggata aataccactg cataaaaaca
      361 tccctttaat taaaagtgac gaaagtctat tccgggtatt gtgtaaatgc tattcgtatc
      421 tttctttttc ggatgttata aaggttttga tatttaagat aaatacatgg ttatttttat
      481 aggtaattta tataacgaca ttgaaatatt ttaaataaac attagtaaat tattcattca
      541 tcaacatgat gataaaagcc ggatgagtca gacatccggc gagcagattt gttttaaggt
      601 tccagttttg gatcaggtcc aaagcggttt tcgcctggcg tacccgcctg gcagttaaag
      661 acgatgataa taagccagcc gataaacggg attaagaata gcagtgccca ccacgccgag
      721 cggtcggtgt cgtgcaggcg gcggaactga actgcccacc acggtaaaaa caccagaata
      781 ccgtagattg tcgtcaggat gccttcgcca ccagcgcgtt gccagcctaa cattttatcc
      841 agcagcccca gcacgaacgt aaagatgatg ttgaccagaa taaacatcca gtactctttg
      901 cgccgcgcac gaccccggaa accgacataa ttttttagta ctttcagata ccagtccatt
      961 tttgcctcaa ttttgctcaa tcacttgttt ttaaagcagc aaattaagaa tagacgtgaa
     1021 tgaaatggca gtaaatattc gttcaaggaa ttaataaagt gctgtgaagg cgcgctaaga
     1081 ctgatgacaa gcgcaaaatt gccagatgcg ctacgcttat ctggcccgcg cggtcattgc
     1141 aacatattga atataaaagt tttgtaggtt tgataaggcg ttcatgccgc atccgacatg
     1201 aacaacatgc acttagctaa ttatcagagg cacctcgtaa ggtgcttttt ttaacctaag
     1261 cgagcatctc gtccatgcgg ttcatcgaga tcctgccacg gaccaattga aataatccgc
     1321 gtagggttga tggtcttatg actgcggaag taatgattac ggatatgatc gaaattgact
     1381 gtttcggcaa ttcccggcat ctggtagata tcacgcagga agccatacag attaaggtaa
     1441 tcgctgatgc ggtgcttatc acacttgaag tgggtcacat acactggatc aaaacggacc
     1501 agcgtggtcc acaggcgaat atcggcttcg gttagctggt tgccggtcag gtaacgatgc
     1561 tgacctaaaa tctgttccag tcgcgccagc gattcaaaca ctttctccac cgcttcgtcg
     1621 taggcttgct ggctggtggc aaaaccagct ttatacacgc cgttgttaac ggtgtcataa
     1681 atccagccgt taagttcgtc aattttcgtt tgcagggctg gtgggtagta atcacccgct
     1741 ttcgcgccca gcgcatcaaa cgcggtatta aacatgcgga tgatttctgc tgattcgttg
     1801 ctgacgatgg tgtggttctt tttgtcccac agcacgggaa cagtaactcg tccgctgtag
     1861 tgtggatcgg cgtggagata aagctgatac aaaaattcat gttgatagag cgtgtctccg
     1921 gttgctcccg gaaaactgtc atcaaaggtc cagccgtttt ccagcatcag cgggttcact
     1981 acggaaacgg aaataaacgg ttccagtcct ttgagtttgc gcatgatcag cgtgcggtgc
     2041 gcccacgggc aggcgagtga aacatagaga tgataacgat ctttctctgc gataaaaccg
     2101 cctgtgccag tggggccagg agcgccatcg gcagtgagcc agttacgaaa tgcggaagct
     2161 gaacgttgaa atttaccgcc ggtagatttg gtatcgtacc aggtgtcatg ccagacgccg
     2221 tcaatcagtt gacccattgt tctctcctcc aatagcaaaa acgaggagct tcctcctcgt
     2281 tttttatcgt taagtatagc gcgtgcttac cactttttat tcagcaggcg gtcgatgcta
     2341 tacgcgcccg gaccggtaat tgccagcagc aggaatccgc cagaaattgt caggtttttc
     2401 atgaacatca gcgagttgac gccttcagca aagttgctgt gaaataaaaa tgccgtcagc
     2461 agcgtaaagc ccgcagtaaa cagggctgtg gtgcgagtca ggaaaccgaa caggattgcc
     2521 agaccaccac caaactcaag cagaatcacc agcggcagca taaaacccgg gacgcccatc
     2581 gcttccatat attgttgggt acccgcgtaa ccagtaattt ttccccagcc tgcggtaata
     2641 aacagaatcg gcattaaaat gcgcgctacc agtacaccaa catcttctaa ttttttcatc
     2701 atactctcca ctgcgccact caggctgctg attgtttttt tgtgcaaggg cgcggtatta
     2761 gcgtcgttgc tgtcgatgga gagaatcata aacgtggtga atgatgattg ttagcaagga
     2821 aaactgtcaa aaatcttcaa aaaatttgat gggtaaggcc ggaatggctc cggccagagg
     2881 gaagttaacc gcgaagctgt tgctgcttga gggtcgtttt aaccagacgc caggcgctcc
     2941 atacgccaaa accgcgtctg gcccagcgga ccagcatatt aggatggcga atcgtccaga
     3001 tcgccatcac gctactgcca accagcgccc aggagcgcag acttagcagc atattccagc
     3061 gacgatcgta agcgcctgtt gcctccagcc attcacgacg actggcagaa agatccagcc
     3121 gttgttgctg gatctggcta agcagttgcg ccttacgtcg ttcacgttcg actttactgc
     3181 tcacgggact cctcctcgag cagctgccga tcgtttgcca gctcatggcg tgtatggcgc
     3241 agcaaggtgg atttacgcga tttacgtaac gtccagatac cgccaatcag tgccagtagc
     3301 agcaacacca cggtggtggc aatcatcgca ttcaggcgat attgcggatc aaccgcccaa
     3361 ataattagca ccatcaggct cataagacca aatgcagcga aaagcatcgt caggcccagc
     3421 atcagtaaaa gttgaaagag attagctttt tcctcttcca gctcaaccac cgccagccgc
     3481 agacgtgttt ccaccatttc aaccatgatg gaaacaattc gctgcccgat gcccagaacg
     3541 cttttaccgg gcccttgcgc gtgatgagtg tccgccataa ttaacgacgc gacagcagaa
     3601 cgccgagcac tacaccgatt gcagcgccaa tgcccacgcc cgtccatgga ttttcacgca
     3661 catactcatc ggcacgcgcc gccgcgacac gggtttgttt ggcgatggca tcaccggttt
     3721 cacccaggcg ataacggctc tgtttcagtg cctgctccgc tttgctacga atcttactca
     3781 actcttcttt cgacttctcg ccagatgagc taagcacctc ttccagcgta tcggaaaggg
     3841 atttcaactc agcacgcaga tgttccgtag tgtgttcttt cgacatagtt ttctccaggt
     3901 gagtaagtag tgactattgt gagttagtag tcgcgcgctt ccagcttttt cagctcttcc
     3961 tgcgcttctg ccagtttccg ttcgcgtttg gcaatcttat cggcatcgcc tttttgcttc
     4021 gcctcggcta aatcttgctg gcgttccgcc acctcatctt tctgctttgc gattttcttc
     4081 tgatgatcgg cacgcagctg gctatctgaa cagttggccc ggacttcact cagggcttta
     4141 ttcagaccgt caatacgatt ctggttttgg tgtttttcgg catagctgat ctccttaagg
     4201 atattttgct ccttttcctg acacagggta gtggcataac taccggcact aagagcaaaa
     4261 agagaaacag ctaaagcgat gcggtatttc attcttgaac cttccattgt gattcggacc
     4321 tattacaaag gaacgattgt ccagacaacg aagcggtagt gttaaccacc gcctcattaa
     4381 tcatagacag ttacggtgaa atcaccaaag tgagtgatct gtttcggaca acaggaagtt
     4441 atccgaagcg atgagagtta tcccgtagcc gggtcagcca ctgcatagcc tgactgttat
     4501 tgtcctgctg cgcaatgatg tagccttggg gcaatgttct gtcgaggacc gcttttgcag
     4561 cggcgctctg gtcgctggag tcaaaggtaa ttaacagagt gtcgtttttg ggggtgatac
     4621 ttttgaaagg aatgccatga gcgtcaaggt gatgccagat tgaaaaaccg tctggcatcg
     4681 ttgtgccttg atgaacggca cgaatcgcca gcgtagactc ttgctggcga accgcggacc
     4741 aggccagcaa cagcgtgccg accagaagta aaacagcgcc ggaccaggct agctggcgaa
     4801 gcgacatgcg aggtatttgc atcccttacc cccgatttcc atatttcttt ttccataata
     4861 cgaccagaga acctgccagg ccaaacacca gcagcaccac cgggagcagc atcaggcatg
     4921 acatcagctg gtcctcgtac tttaaaaata ccggcgtttt gccgagcatg taacccagag
     4981 ttgtcaggat caatacccac agcagaccgc tcatccagtt gaaaaactga aagcgcgcgt
     5041 tattcagccc tgataacccg gcaatcgtcg gcagcagtgt tctgacaaac gcaataaagc
     5101 gaccaattaa cagcgccgat aaaccgtgtt tatgaaaaag atggtgtgcg cgttgatgat
     5161 aatgcgcggg taaatgagat agccagtttt gtacggtgcg ggtattgccc agccatcgcc
     5221 cctgaatata gctgacccag cagccgaggc tggcggcaac ggtcagcagc agaatcgttt
     5281 gcggatagcc catcgcgcct ttcgcaatca acacgccgac caataccagt aaactgtcgc
     5341 ccggtaaaaa ggccgccgga agcaagccgt tttcaaggaa caaaattaca aacaagacaa
     5401 aatacaacat gccaatcatc gatggattgg ccagggtttc aaaatcctgc gcccacaggg
     5461 cttgcagcaa ttgggtcaaa agttccattc attattcctg gtgaatcggt taacgccacg
     5521 ctggttttaa aagatgcact accgcaatgt tattgctgtt ttggtatgtt ctgcgggttt
     5581 gatacatcaa cacagcctga actattccct gttaaatcgt gagataagaa agcattcaaa
     5641 aacataaatt caaattgaat ctaattgtaa caaaggtcaa cgttgttcgt tatcaaaatt
     5701 acatgtccgt ggagtctgat tttgctgaat gcaggggagg gtggcgagag gatttacaca
     5761 ggctgacact ttatactttt cgcttaccga cgagacaaac aggcgcgtcg cagcgcctgt
     5821 cggacaggta tttcatttac tgccactggt ggccgtatcg agatgcacta ccgggttttc
     5881 ggcgaacaga tagcggtcgg cattgaactc gaagtcgtcg ctggtttcgt tgaataacat
     5941 gatcttggtg ttttccaggt gctgccacat tgccagctta gcagcatgag gatctttgcg
     6001 aatcagcgcc ttgaggattt gatcgtggtc atcacaccag ttatcgacgg tacgggaatc
     6061 aatgtgttcg tgcagttttt tccagtacgg gttatggcta cgctgggtcc acattttttc
     6121 aacgatagcc gccagggcgg agttctgcgt cgccagggct acctgaatat ggaactgcaa
     6181 atcccactcg gaatcacgga agcattgttc gccgcgcgcc tgctcctgga tggccatcaa
     6241 tttcatgatg tcctgtttcg ttacctgagt tgctgcgaac tcggcaatat tactttcgat
     6301 gagttggcga gcctggagta actcaaacgg accgtaattg gcgaattcca tattattgtc
     6361 cgcagcctgc tgatggcgcg gctggttgga aaccacgtga atacctgaac ctttacgcac
     6421 ttcgacatag ccttcaactt ccagcatgat gatggcttca cgaactaccg tacggctgac
     6481 gttcttttca tcggcaataa agcgctcagc aggcagttta tcacccacca gatagacgcc
     6541 ctgttcgatg cgctctttca ggtcagcggc aagttgttga tacaaacggc gtggttcagt
     6601 gatttccata tgcgctccag gcatgatacg gcagactata tttgttatac cacttttgcg
     6661 agtttagctc tactcgcagt gatgaaaaag ccgcctgaga aggcggcttt gaagcgaaac
     6721 taaaacttac acgaagcgtt aatgttgcgg tgcgggatcg ttatgtgttt cctgcgccac
     6781 ctcaatcgcc ggtttgttct gcaatacggt ccagataacc agcgcaccca gcaggtcaaa
     6841 tactgccaga actgcgaaca gcgggctgaa gccgatggtg tcagccagtg caccgacaac
     6901 cagcgcaaac agcgtacttg ccagccatgc ggacatcccg gttaaaccgt tcgccgttgc
     6961 cacttcgtta cgaccaaaca cgtcggaaga gagcgtaatc agcgcgccag acagtgcctg
     7021 gtgggcaaaa ccaccgatac acagcagcat aattgcgaca tacgggttgg tgaacaggcc
     7081 gatcataccc gggccaatca tcagcactgc acccagcgtt acgaccatct tacgggaaac
     7141 gatcaggttc acaccaaacc aacgctggaa cagcggcggc aggtaaccac cgaggataca
     7201 accgaggtca gcaaacagca tcggcatcca ggcgaacatc gcgatctctt tcaggttaaa
     7261 gccgtaaact ttaaacatga acagcgggat ccacgcgtta aaagtacccc aggccggttc
     7321 tgccaggaaa cgcggcagcg cgatacccca gaactgacgg ttacgcagga tctgaccaac
     7381 ggacattttc ttcgccgtac tcacctggtg ctgggcttcc tgaccattaa taatatagtc
     7441 gcgttcttca tcggtcagat gcttctggtc gcgcggatgt ttatagaaaa tcagccatgc
     7501 catcgcccag ataaagctca atgcaccgga gatgataaat gccatctgcc agctgtgcat
     7561 cacgattgcc cataccacca gcggcggcgc aatcatcgca ccaatcgaag aacctacgtt
     7621 aaaatagcct actgcgatgg aacgctcttt cgccgggaac cattcggagc tggctttcag
     7681 acccgccggg atcatcgctg cttccgcggc accgaccgca ccacgagcaa cagccaggcc
     7741 accccagcta cctgccagcg cggttgcacc acagaacacg gcccacagca cagcaaacat
     7801 tgcataaccg attttcgtac ccagcacatc cagtacataa cctgctaccg gttgcatgac
     7861 cgtataagca gcagaatagg ctgcgatgat ataggaatac tgttgggtgg agatgtttaa
     7921 ctcttccatc agagttggcg cagctgccgc cacagtgtta cgcgtcaggt aaccaagcac
     7981 ggtgccgagc gtcaccagtg cgatcatata ccaacgtaac cctttaattt tacgcatgta
     8041 aaacctcatc gttttgttat atccgcagga agcgcggcca tcatcccgcc agaggctgaa
     8101 gatgttagtt acgaccgggt aaccgaaaaa aaaccggcac gccccgaacg ttgccatgaa
     8161 agtttcgtgc taattcggct tccgtaccgg ttaacccgat tcatctaaca aatgaatgaa
     8221 tcggcaattt gcattccgtc gacttatgat ttgcgacggc agaaagataa cttgtcatac
     8281 aactttaaaa ggtgagagcc atcacaaatg tgcgaatatt tgtagggaca ttacctgacg
     8341 acagcgaggc cagtactggc gcggcctgca gagagattta ccactttgag agtaattttt
     8401 ttaactacgt ttattgatct aactcacgaa aatatcttcg gactctggaa attggtgtga
     8461 taactttgtc agcatcgcac cataagcaag ctagctcact cgttgagagg aagacgaaaa
     8521 tgactccgtt tatgactgaa gatttcctgt tagataccga atttgcccgc cgtctgtatc
     8581 acgactacgc aaaagaccag ccgattttcg attaccattg ccatttgccg ccgcagcaga
     8641 ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg gctgaaaggc gatcactaca
     8701 aatggcgcgc tatgcgtacc aacggtgtgg ccgagcgtct gtgtaccggt gatgcgtctg
     8761 accgtgaaaa atttgacgcc tgggcggcga ctgttccgca cactatcggc aacccgttat
     8821 accactggac gcacctcgaa ctgcgtcgtc cgtttggtat cactggcaaa ttgctttctc
     8881 cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact gctggcgcag gataacttct
     8941 ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt cggcaccacc gatgacccga
     9001 tcgattctct ggagcatcac gcagagatcg ccaaagacgg ctctttcacc atcaaagtgc
     9061 tgccgagctg gcgtccggac aaagccttca acatcgaaca ggcgaccttt aacgactaca
     9121 tggcgaagct gggcgaagtt tccgataccg acattcgccg ctttgctgac ctgcaaactg
     9181 ccttgaccaa acgtctggat cacttcgccg ctcacggctg taaagtgtct gaccatgcgc
     9241 tggacgtggt gatgtttgct gaagcgaacg aagcggaatt ggacagcatt ctggcgcgcc
     9301 gtctggctgg cgaaaccctg agcgagcacg aagtggcaca gttcaaaact gcggtactgg
     9361 tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca gcagtaccac attggcgcgc
     9421 tgcgtaataa caacctgcgt cagttcaaac tgctggggcc ggatgtaggc tttgactcca
     9481 tcaacgaccg tccgatggca gaagagttgt ctaagctgct gagcaagcag aacgaagaaa
     9541 acctgctgcc gaaaaccatt ctgtactgtc tgaacccgcg cgataacgaa gtgctgggca
     9601 ccatgagcgg taacttccag ggcgaaggta tgccgggcaa aatgcagttc ggttccggct
     9661 ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat gacccaactg gcgcagctcg
     9721 gtttgctgag ccgctttgtc ggtatgctga ctgacagccg tagcttcctg tcatacaccc
     9781 gtcacgaata cttccgccgc attctgtgcc agatgatcgg tcgctgggtg gaagcgggcg
     9841 aagcaccggc ggacatcaac ctgctgggcg agatggtgaa aaatatttgc tttaacaatg
     9901 cgcgtgacta cttcgccatt gaactgaact aaggtctggg gttgatatgc aatacatcaa
     9961 gatccatgcg ctggataacg tcgcggtcgc tttagcggat ttggctgaag gcacagaagt
    10021 cagtgtcgat aaccaaactg ttaggctgcg ccaggatgtt gctcgtggac ataaatttgc
    10081 gttaacgaat atcgcaaaag gggccaacgt catcaaatat ggcctgccga ttggttatgc
    10141 attggcggat attgcggcgg gggaacacgt tcacgcccac aatacgcgca cgaatctgag
    10201 cgatctggat cagtatcgct atcaacctga ttttcaggat ctgcctgcgc aagcggcaga
    10261 tcgtgaagtg cagatctatc gtcgcgctaa cggcgatgtc ggggtgcgta acgagctgtg
    10321 gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg cagatccaga accgttttct
    10381 gaaagagacc aacaacgctg aaggtaccga cggcgtgttc ctcttcagcc acacctacgg
    10441 ctgctcacag ctgggcgacg atcacattaa tacccgcacc atgctgcaaa acatggtgcg
    10501 ccacccgaac gcaggcgcag tgctggtgat tggtctgggc tgtgaaaaca accaggttgc
    10561 cgcattccgt gaaacgctgg gcgatatcga tcctgaacgc gttcatttca tgatctgcca
    10621 acagcaggat gatgagatcg aagccggaat cgagcatttg catcagctgt ataacgtgat
    10681 gcgcaacgat aaacgcgagc caggcaaact cagcgaactg aagtttggtc tggagtgcgg
    10741 tggttctgac ggtctttctg gtattactgc taacccgatg ctggggcgtt tctctgacta
    10801 cgtgattgct aacggcggta ctaccgtact gaccgaagtg ccggagatgt ttggcgcaga
    10861 gcagttgctg atggaccatt gccgcgacga agcaacgttt gaaaaactgg tcaccatggt
    10921 caacgacttc aaacagtact ttattgccca tgaccagccg atctatgaaa acccatcgcc
    10981 ggggaacaaa gcgggcggta tcaccacgct ggaagataaa tcccttggct gtacccagaa
    11041 agcgggttcc agcgtcgtgg ttgacgtgct gcgttacggc gagcgtctga aaacgccggg
    11101 gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg accagcgccc tggcgggtgc
    11161 gggctgccat atggtgctgt tcagtactgg tcgtggcacg ccatacggcg gctttgtacc
    11221 gacggtgaaa atcgctacca acagtgaact ggcggcgaag aaaaaacact ggatcgactt
    11281 tgacgcgggt cagctgatcc acggtaaagc gatgccgcag ttgctggaag aatttatcga
    11341 taccatcgtt gagtttgcca acggtaaaca aacctgcaac gagcgtaacg acttccgcga
    11401 actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga aatgagttgt taataaaaaa
    11461 cggcgtttca taatgtgaag cgccgtttgc ttttccatac ctgataagga tttatcatga
    11521 ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt tctgattggt atcacaacat
    11581 ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc ggtctatagc gccgttattg
    11641 gcgtacattt ttttcttctg ggcacctatc ccgctggtgc cagtgccatc cttaatgcca
    11701 ttcgtacatt gattacctta cgcacgcgca gcttatgggt aatggcgatt tttattgtgc
    11761 tgactggcgg aattggcctc gcgaagttcc atcatcctgt cgaactattg ccggttatcg
    11821 gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg gctcaccatg cgctgcgtga
    11881 tgtggttttc aacgtgttgc tgggtgattc acaacttctg ggcggggtcg ataggcggca
    11941 cgatgattga gggtagtttt ctgcttatga atggcctgaa tatcattcgt ttctggcgga
    12001 tgcagaaaag gggaattgat ccgtttaaag tagagaaaac ccctcccgcc atagacgaaa
    12061 ggggttaaac aattaattac gcagggcgct atttgccaga cgatcgtctt ccgcctggca
    12121 agctgccgca gtgaacagca cgtcagttga agagttcagc gcggtttcgc aagagtcctg
    12181 caatacgccg ataataaagc cgacggcaac cacctgcatg gcgatatcgt tcgaaatacc
    12241 gaacatatta caggccagtg ggatcagcag caaagaaccc cccgccacgc cggatgcgcc
    12301 acaggcacac agagaagcca ctacgctcaa cagcagcgct gtgggcagat cgaccggaat
    12361 acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg cgcccgccat
    12421 attgatggtg gctcccagcg gaatagaaac ggaataggta tcgcgatcca gattcagctt
    12481 ttcacacagt gccatattca ccggaatgtt cgccgcagag ctgcgggtga agaaggcata
    12541 cacgccgctt tcgcgcaggc acagcagcac cagcgggaac gggttacgac gaattttcca
    12601 ccacaccagc aatgggttaa ccaccagcgc caccagtaac atacagccaa ccagcacgac
    12661 cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg gttgccaggg tagaagaaac
    12721 cagaccaaaa ataccaatcg gtgcgaagcg aatgaccagt ttcaccataa aggtgacggc
    12781 attcgacata tcgttgacca ggtttttggt ggtctcgtta ccgtgacgca gtgcgaaacc
    12841 gaggccaatt gcccacacca ggatcccgat gtagttacct ttcagcagcg cgtcgatggg
    12901 gttggaaacc atgctcatta ccagcccgcg catcacttct acaatgcctg acggtggcga
    12961 aatatcaccc gcgctactgg ataagtgcag ggtagaaggg aaggcgaagc tgaaaacgac
    13021 tgcggccaga gcagcagaga aggtacccag cagatagagg aacaaaatag ggcggatatt
    13081 ggttttctgc ccgtgctggt ggttagcaat agatgccatc accagcatca acaccaggat
    13141 gggggcaacg gctttcagtg cgccgacgaa caaagtacct aacagaccaa cagcttccgc
    13201 cgcgggtttt gagatccatg ccagaagaat ccccagaaca aggccgacca ggatttgttt
    13261 taccaggctg ccatgagcca gacgccggaa tagccccggt gaacgttgcg tagtcatttt
    13321 tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc atctttcagg
    13381 atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg gaagcgaaaa
    13441 atgctggatt ttaaagagag gtcatatttt gcaacttaat ggttacattt atttgacata
    13501 aaaataaagc caacgtgcgg cttccagact gaggactacg tcaaaaaagg actcggtcag
    13561 tcccctcgcc ccgtttgggg agagggttag ggtgagggga acaggccagc atcggtgcga
    13621 aaaattaccc tcaccccgac cctctccctg gaagggagag ggagaaaacg gtgctcatgt
    13681 tgatgacagg gtacacggct taggcagatt aaaaatcatc caacccgctg cttatcatgc
    13741 cgataattca cccaggcgtt gataataaac gtcatcacca ggatgccaaa caccacgccc
    13801 agcgagacgg cgattggaat atggtagaag tcgacaatca gcatcttgat accgataaac
    13861 accagaatca ccgccaggcc atatttgagc atcgagaaac gctctgctac gcccgccagc
    13921 aggaaataca tcgcacgcag gccgaggatc gcaaacaggt ttgaggtcag cacaatgaac
    13981 gggtcagtgg tcacggcgaa gatagccgga atgctatcca cggcaaaaat cacgtcgctc
    14041 aattccacca gaatcagtac cagcatcagc ggtgtggcgt acagcaatcc attcttacgc
    14101 acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca aatgaccgcg tagccagcgc
    14161 accagcggct tgtcgccaat gcctgattca tcttcatggg cgagcgccat cttcacgccg
    14221 gtaaacagca ggaaggcacc gaagatatac aggatccagt cgaactgcga aatcagccag
    14281 ctgccagtga agatcatgat ggtacgcaga acaatcgccc cgagcacgcc atacaccagc
    14341 acgcggcgtt gtaatgccgc cggaacggag aaatagctga acaacatcag ccagacaaag
    14401 acgttatcga ccgccagcga cttctcaatc agataacccg tgaggaaggc cagcgcctgt
    14461 ggatcggcaa cggcgcgacc ttcggtttgt accagatacc accagaaagc agcgttaaac
    14521 agtaacgaca gcgtcaccca gaccagcgac caggccgcag cctgtttcat ggtcatggca
    14581 tgtgccccac gacgtccctg caacaacagg tcgatagcca gcataatggc gacaacaaca
    14641 gcgaatccgc cccatagcaa cggcgtgccg acagtattca tagaagttcc ttacacataa
    14701 aaaaaacggc caacgtcgga agacgtcagc cgctgctttt tatgcataga cctcgccttc
    14761 cggcaaggtc tcacttacaa caaaaaagga atacgtctgc gtattccttt cgggttgccc
    14821 ggtgaccgga tgtggttttt cacacatcgt aatgacgatc gaccggcaat gaagttactc
    14881 ccctttgcag gtaacaaagt atgacagacc attcagtcag tcaatggtct gttacatacc
    14941 ttttacactg ttttactttg gcaattatag ttttacgcta tctgccggaa aaatcacccc
    15001 agtctggcgg cggatctcgg tcagcagttt ggctgtgatg cgactgaccg ccagccctgg
    15061 atgatccacc agatgctcat ccaccagggt agcgaacagc tctgcttcgt agagcatggt
    15121 attaatatgc tgcggctggg tgagatcctg catctggctg ccacgcggca cgaagcacac
    15181 tttctggcat tcagacagtt tttcaatcac cagcgatccc gcttcgccct gaatctcgct
    15241 tgccaggaca gaatcgctga cctttgagtg ctgcaaggtg acgctgaaat caccgtaatc
    15301 catcaccacc acgccgtggg catctacacc acttgccagt aaactggcgg ttgcctgcac
    15361 gcttttcggc tcaccaaata acgctaccgc cgacgccagg cagtaaaagc cgatatccat
    15421 aattgaaccg ttagagaatg ccggattaaa ggtgttggga ttctcaccgt ccaggtaacg
    15481 ttgataccgc gaggaatact ggcaatagtt gaaaaaaact ttccgcagtt tgccgacttt
    15541 cggcagcgcc tggcgtaaca gatgaaagtt aggcaggctg gccgttttaa atgcctcaaa
    15601 cagcaccacc tgattttcac gcgcgcaggc aatggctgca tccacttccg ccagattcga
    15661 cgccagtggt ttctcacaaa tcacatgaat tttgtggctc aggaaaagtt gcgtctggga
    15721 aaaatgcagg gcgttcgggc tggcaatata caccgcgtcg atagcgtcac tttccgccat
    15781 tgcttccagc gaagtaaaca gatgctcgac agaaaactca ttggcgaagt gctgggcctg
    15841 ttcaaggctg cgggaataaa cggcggttaa cttatattta ccgctttcat gtgcggcctc
    15901 cacgaactgg tgagttatcc agttcgtacc aatcacggcg aaacgtatca taacgcgtgg
    15961 atactccata aagggattct gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc
    16021 ggatacgcat tatttctgcg ccggacaacc atgctcacta aacggaccaa taatgaccga
    16081 cggcccgaga tattttggct gacgattgaa gattttcgcg tcaatcaccg cgctgactct
    16141 ggcgagtttc tctcgcagac ggacaatctt tccgcgtcct ttttccaggt ctggtgcgtt
    16201 aaacccgata gcatcgatgc cgtattgttt tgccagccag atggcgcgtt ggttatgaaa
    16261 ttcctgcgag ataatggtga tatggttctc cccaaacact ttattggcac gcaccactga
    16321 atcaagcgtt gagaatccgg catagtcaca gaagatcact tttgccggta cgccctttgc
    16381 gatcaaagcc tgctgcatgc ctgacgcttc atcgtaattt ttacgtccgt tatcgccact
    16441 gaccagcagc catttcactt ttccggcgtg gtacaactct gctgcggtat caatacgtcg
    16501 ggtaaagtag cgattacccg gcctggcccc cagcaataag cccacgttac gcgccggaac
    16561 gacgttgacg tcgctccagg tcagctgttt gctggcgttg accattacgc gatcggcgat
    16621 aaaaatggtc gctcctgcca caagtaacag taagcaggca atctttagag tccggcgaga
    16681 aaaacaaata cggagaagaa ggcgggcaaa tgcgcgcagc atggtcgtta tccttaacga
    16741 aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc
    16801 agattttgtc ctgcccaagc gacaactggg ttagccacag tcgggtgtcg aactcaagct
    16861 ggtggtactg gggttccata tgacagcaca actggtaaaa cgctttgtta tgctctttct
    16921 ccttcaggtg cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc ggcgcgttgc
    16981 gaaacacggt agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcacacgag
    17041 aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca
    17101 ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga gtatattgcc
    17161 agagggcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc
    17221 ccagacgctg ttcgttaatc agtgttcgca cctgggaaag tagctgctcg ggataaccct
    17281 gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc
    17341 acccttttca ggggcaatgt cgaaatttta tcattcagga gggccgatga gccacttaga
    17401 caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct
    17461 acaggcgtgg gaagcggcgg atgaatattt gctgcaacag ttggacgaca cagaaatccg
    17521 cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca
    17581 taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg
    17641 cctcaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca
    17701 acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact
    17761 gcgtgcgctg cgcaaagtgg tcacgccaca aacacgtatt attgccggtg ccaaagcccg
    17821 tgacatccac acttccacgc tggaactatt cgaaaaagtg ctcggcccga ccaccaccac
    17881 gctggcatgg aagaaagcgc gcctgattaa ctgcactttc aatgaaccac cgctggccga
    17941 tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc acaaccatgc
    18001 gaatgtcttc tcccgcaccg ggctggatat cggcgcgcgc ttctttatgc aacatctgcc
    18061 agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta ttggtctgac
    18121 gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt
    18181 tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc gctgcgaatt
    18241 tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa
    18301 cccgccgttt catcagcaac atgcgctgac cgataacgtc gcctgggaga tgttccacca
    18361 cgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc gtcacctgga
    18421 ttacttccat aaactgaaga aaattttcgg caactgcacc accatcgcca cgaataataa
    18481 atttgtggtg ctgaaagcag tgaagctggg gcgtcgtcgg taagattgac agctacctgc
    18541 cagataaggc gtaaacgcct tatctggcat acgtttagca cattaccccc aggtcggata
    18601 agatgcggca agcgtcgcat ccggcaatgt ctgcccagtt ctggaatcgc gctccataaa
    18661 acaaaaaatc cctgtcacca tcctctacat tctcttgttt agcgttgttt aacgtttatt
    18721 cttccgtcac acagataaat tcatccgttg ccaatctgtc aacggatgtt atcatattgc
    18781 caatttgtca acggacgtga tgaagaatgc acctgataac tcaaaaagca ttgaaagatg
    18841 ctgcggaaaa atacccgcaa cataaaacgg agttggtggc tctagggaac acgattgcta
    18901 agggatattt caaaaaacct gagtcattaa aagcagtatt cccatctctg gataacttca
    18961 aatatctgga taagcattat gttttcaatg ttgggggcaa tgaattacgt gttgtagcaa
    19021 tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt tatgacgcat aaagaatacg
    19081 atttttttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg ccgacatctt
    19141 gcatgcagga gaaaagctaa ctgctgtggc accttttctg gcgggtattc agaacgagga
    19201 acaatacatc caggcgctgg aactggtaga tcatctgctg ctcaacgatc ctgaaaaccc
    19261 cttactggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc ccgaatttgc
    19321 ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc gtacccttat
    19381 ggatcaatat ggtttaaccc tttccgatct gccggaaatt ggcagtaaat ctatggtgtc
    19441 acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat tggcaacgcg
    19501 attcggcatt tctcccgcct tgtttattga ttaacgcga
//
LOCUS       DAZKIF010000094        19306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZKIF010000094 DAZKIF010000000
VERSION     DAZKIF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.094"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            30..548
                     /locus_tag="ACHOTW_003273"
     CDS             30..548
                     /locus_tag="ACHOTW_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9845323.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            564..1103
                     /locus_tag="ACHOTW_003274"
     CDS             564..1103
                     /locus_tag="ACHOTW_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP9845324.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1196..2773)
                     /gene="guaA"
                     /locus_tag="ACHOTW_003275"
     CDS             complement(1196..2773)
                     /gene="guaA"
                     /locus_tag="ACHOTW_003275"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9845325.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2842..4308)
                     /gene="guaB"
                     /locus_tag="ACHOTW_003276"
     CDS             complement(2842..4308)
                     /gene="guaB"
                     /locus_tag="ACHOTW_003276"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9845326.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4470..5840
                     /gene="xseA"
                     /locus_tag="ACHOTW_003277"
     CDS             4470..5840
                     /gene="xseA"
                     /locus_tag="ACHOTW_003277"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9845327.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5837..6052)
                     /locus_tag="ACHOTW_003278"
     CDS             complement(5837..6052)
                     /locus_tag="ACHOTW_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9845328.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6121..7593)
                     /gene="der"
                     /locus_tag="ACHOTW_003279"
     CDS             complement(6121..7593)
                     /gene="der"
                     /locus_tag="ACHOTW_003279"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9845329.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7711..8889)
                     /gene="bamB"
                     /locus_tag="ACHOTW_003280"
     CDS             complement(7711..8889)
                     /gene="bamB"
                     /locus_tag="ACHOTW_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP9845330.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8900..9520)
                     /gene="yfgM"
                     /locus_tag="ACHOTW_003281"
     CDS             complement(8900..9520)
                     /gene="yfgM"
                     /locus_tag="ACHOTW_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP9845331.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9538..10812)
                     /gene="hisS"
                     /locus_tag="ACHOTW_003282"
     CDS             complement(9538..10812)
                     /gene="hisS"
                     /locus_tag="ACHOTW_003282"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9845332.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10923..12041)
                     /gene="ispG"
                     /locus_tag="ACHOTW_003283"
                     /gene_synonym="gcpE"
     CDS             complement(10923..12041)
                     /gene="ispG"
                     /locus_tag="ACHOTW_003283"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP9845333.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12068..13081)
                     /gene="rodZ"
                     /locus_tag="ACHOTW_003284"
     CDS             complement(12068..13081)
                     /gene="rodZ"
                     /locus_tag="ACHOTW_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP9845334.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13366..14520)
                     /locus_tag="ACHOTW_003285"
     CDS             complement(13366..14520)
                     /locus_tag="ACHOTW_003285"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP9845335.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14670..15101)
                     /gene="ndk"
                     /locus_tag="ACHOTW_003286"
     CDS             complement(14670..15101)
                     /gene="ndk"
                     /locus_tag="ACHOTW_003286"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9845336.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15242..16096)
                     /locus_tag="ACHOTW_003287"
     CDS             complement(15242..16096)
                     /locus_tag="ACHOTW_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9845337.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(16096..16917)
                     /locus_tag="ACHOTW_003288"
     CDS             complement(16096..16917)
                     /locus_tag="ACHOTW_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFP9845338.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16910..17539)
                     /locus_tag="ACHOTW_003289"
     CDS             complement(16910..17539)
                     /locus_tag="ACHOTW_003289"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFP9845339.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17536..>19306)
                     /locus_tag="ACHOTW_003290"
     CDS             complement(17536..>19306)
                     /locus_tag="ACHOTW_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFP9845340.1"
                     /translation="TPYTYGVAASGNSMDTLLDTKLVILWGHNPTETIFGHTNYYFQK
                     MKQNGTRFIVVDPRYSDTVSSLADQWIPLLPTTDNALMDAMMYVIISENLHDKTFIDT
                     YTLGFDENSMPEGVPANESLVAYLFGAKDGIHKTPEWAEKITHVPAQSIRQLARDYAT
                     TKPAALIQGWGPQRHICGERTARGSTLLASITGNVGIKGGWAAGYGGSSNRKFCVGPD
                     MPENPVQAKISIMNWMQAADDASKVTPQDGLKGVDKLDSNIRLLFSLAGNYLANQNPD
                     VHQAAKLLEDESKIEFIVLSDLFMTPSAKYADVLLPETSFMERWNIGETWGTASYLIL
                     SEKPIEPDFERRTDYDWLRDVAKKLGVEAEFSQGRDEKQWIEHIWEQTRLAMPDENLP
                     DFATLQKTRRHLFKSAPHIAFEANIRDPQNNPFPTPSGKIEIFSKRLFDMQDPEIPAL
                     SHYVPAFEGPEDKLTAKYPLQLITWKGKNRANSTQYANPWLQEVQTQKLWLNPQDAKQ
                     RGISEGDSVKIYNDRGVSIIPVEITPRIIPGVVAMQAGAWWQPDAQGIDRGGCANVLS
                     STRITALAKGNSHQTMLVEVEKA"
BASE COUNT         4765 a         5247 c         4704 g         4590 t
ORIGIN      
        1 atttttcgta atgtgtataa caaggaatag tgatgaaatt taaaaaatgt cttctgcctg
       61 tggcaatgtt agcgtcattc actctggcag gatgccagtc aaatgctgac gatcatgccg
      121 ccgatgttta tcaaaccgat caactgaata ccaaacaaga aactaaaacc gttaatatta
      181 tttccattct tcccgcaaaa gttgccgtag acaactccca aaataaacgg aacgcacaag
      241 ccttcggcgc gcttattggc gcagtcgctg gcggtgttat cggccacaac gtcgggtctg
      301 gcagcaattc cggaacgacg gcaggtgcag ttggcggcgg agctgtaggc gcggcagcgg
      361 gttctatggt gaatgataaa accttagtgg aaggtgtttc tttaacgtat aaggaaggca
      421 ccaaagtgta tacctctact caggtgggta aagagtgcca gtttacgaca ggtttagccg
      481 ttgttattac cacgacgtat aacgaaacgc gtattcagcc aaataccaaa tgtcctgaaa
      541 agagctaata atcaggagga gtcatgaaga aagtttttct ttgcgccatc ttagcctcct
      601 taagctatcc ggctatcgcc tcatcattgc aggatcaact ctctgctgtc gcagaagcgg
      661 aacagcaagg taaaaatgaa gagcaaaggc agcatgacga atgggtcgcg gagcgcaaca
      721 gggaaatcca gcaagagaag caacgtcgcg caaatgccca ggctgccgct aacaaaagag
      781 cggcaacggc agcggcaaat aagaaagctc gtcaggataa actggacgcc gaagccactg
      841 cggacaaaaa acgcgatcaa agttatgaag atgagctacg cagcttagag attcagaaac
      901 aaaaactggc gctggcaaaa gaagaagccc gcgttaagcg agaaaacgaa tttatcgatc
      961 aggagctgaa gcacaaagcc gcgcaaactg atgtcgtgca atctgaagct gacgccaaca
     1021 gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt gggcaaagca gaagagaata
     1081 aatcggacag ctggtttaat taatcgatgt tagtaacttc aagcctataa ttcttgaaga
     1141 taaaaaaccc tctgtagtaa cagagggttt tgttcattca tagtgcaggg tcaaatcatt
     1201 cccactcaat ggtagctggt ggcttgccgc tgatgtcata caccacgcgg gaaataccgt
     1261 tcacttcatt gataatgcgg ttggaaacgc gaccgaggaa atcgtacggc agatgcgccc
     1321 agtgtgcggt cataaagtcg atggtttcga cagcacgcag agagacaacc cagtcatact
     1381 tacgaccatc gcccattacg ccaacggaac gtactggcag gaagacggtg aacgcctggc
     1441 tgactttgtc gtacaggtcc gctttacgca gttcttcaat gaagatggcg tcagcacggc
     1501 gcagcaggtc acagtactct ttcttcactt cacccagaac acgaacgcca aggcctggtc
     1561 ccgggaacgg gtgacggtac agcatgtcgt acggcaggcc cagctccaga ccaatcttac
     1621 gcacttcgtc tttgaacagc tctttcagcg gttcaaccag gcccatcttc atctctttcg
     1681 gcaggccgcc cacgttgtgg tgagatttga tgacgtgtgc tttaccggtt gcagacgccg
     1741 cagattcgat aacgtcaggg tagatggtgc cctgcgccag ccacttcacg tcttccagtt
     1801 tcagcgcttc ttcatcgaat acttcaacga aaacgcgacc gatgatttta cgttttgctt
     1861 ccggatcgtt ttcgccagcc agcgctgaca ggaagcgatc ttctgccggt acgtgaacaa
     1921 tgttaagacc aaagtgatcg ccaaacatat ccagaacctg ctctgcttcg ttgagacgca
     1981 gcaggccgtt gtcgacgaat acgcaagtca ggtttttacc gatagcgcgg tgcagcagca
     2041 ttgcggttac ggaggaatcc acaccaccag agaggccgag gatgacttta tcgtcgccta
     2101 cctgctcgcg gatgcgagct acagcatcgt cgataatttt cgctggcgtc cacagggctt
     2161 cacactggca gatatcacgc acaaaacgct ccagcatgcg cataccctgg cgggtgtgag
     2221 tcacttccgg gtggaactgt acgccataga agcgtttttc ttcgttagcc ataatggcaa
     2281 acgggcagct ttcggtgctg gctacggtga cgaagtcgga cgggatagcg gtaactttat
     2341 cgccgtggct catccagaca tcgagcagcg gtttaccgtc tgcggtcagc gcatcttcga
     2401 taccgcgaac cagtgcgctg tcgtttacga cttcaacctg cgcgtagcca aattcacgtt
     2461 cgttagaggc ttcaacgtga ccgcccaact gcattgccat ggtctgcatg ccatagcaaa
     2521 cgccgaatac cggtacgcct gcttcaaaga catactgcgg cgcacgcggg ctgttttctt
     2581 cagtagtact ttccgggccg ccggaaagaa taatgccgct tggattgaag tcacgaattt
     2641 gtgcttctgt cacatcccat gcccacagtt cgcagtaaac acccagctca cgcacgcggc
     2701 gcgcaaccag ttgagtgtac tgagaaccga agtccagaat gaggatgcga tgcttatgaa
     2761 tgttttccgt cattgacgct tattccgagg caagtgaaac agataatata aatcgcccga
     2821 catgaagtcg ggcgaagaga atcaggagcc cagacggtag ttcggggact ctttagtaat
     2881 ggtcacgtcg tgaacgtggc tttcctgaat gcccgcaccg ctgatacgta caaactccgc
     2941 tttagtacgc agttcgtcga tagtaccaca gccggtcaaa cccatacagg agcgcaggcc
     3001 gcccatctgc tggtgaatga tctctttcag gcgaccttta taagctacgc gaccttcgat
     3061 accttccggc accagtttgt cggcagcgtt atcgctctgg aaataacggt cagaggaacc
     3121 tttggacatc gcgcccaggg aacccatacc acggtaagat ttgtaagaac ggccctggta
     3181 gagttcgatt tcacccggag attcttcagt acccgccagc atggaaccta ccatcaccgc
     3241 gcttgcgcca gcggcgatag ctttggcgat gtcgccggag aagcgaatac caccatctgc
     3301 gataaccgga ataccggtgc cttccagggc ttcaactgcg tcagcaacgg cagtgatctg
     3361 cggaacacct acgccagtaa cgatacgagt cgtacagata gaaccagggc cgataccgac
     3421 tttaaccgcg ctgcaaccag cttctgccag agcgcgtgca cctgcagctg ttgccacgtt
     3481 gccgccgatg atttgcagat ccggatattt agcacgggtt tcacggatac gctgcaaaac
     3541 gccttctgag tgaccgtggg aggagtcgat cagcagaacg tcaacgccag cggcaaccag
     3601 cgcgtcaaca cgctcttcgt tacccgcacc tgcgccaact gcagcaccaa cacgcagacg
     3661 gccttgctcg tctttacagg cgttcggttt acgttccgct ttctggaaat ctttcacggt
     3721 gatcatgccg atcaggtgga attcgtcatc aaccaccagc gctttttcaa cgcgtttttc
     3781 gtgcattttt gccagcacca cttcacgggc ttcaccttca cgcacggtga ccagacgctc
     3841 tttcggcgtc atgtaaacgc taaccggctg gttcaggtcg gtaacaaaac gcacgtcacg
     3901 accggtgata atccccacca gttcgttttc ttcggtaacg accggatagc ccgcaaaacc
     3961 gttacgctcg gtcagttctt tcacttcgcg cagcgtcgtg gttggcagaa cagtctgcgg
     4021 atcagtcacc acaccagatt cgtgtttttt cacacggcga acttcttctg cctggcgttc
     4081 aatggacatg tttttgtgga taaagccgat accgccttcc tgagccagag caatagccag
     4141 gcgcgcttcc gttacggtat ccattgctgc ggaaagcata gggatattca gacgaatagt
     4201 tttcgtcagc tgggtgctga ggtcagcagt attcggcaga acggtagaat gagcaggaac
     4261 gaggagaacg tcgtcaaacg tcagagcttc tttagcgata cgtagcatgg gcaatatctc
     4321 gaccagagtg gttaataaat attgccgcgg cattatacag agcgtaaccg attgcatcta
     4381 cccctttttg caaaaaatgc ttgctatccc cgaagggcgg gttactatcg actgaataac
     4441 ctgctgattt agaatttgat ctcgctcaca tgttaccttc tcaatcccct gcaattttta
     4501 ccgttagtcg cctgaatcaa acggttcgtc tgctgcttga gcatgagatg ggacaggttt
     4561 ggatcagcgg cgaaatctct aatttcacac aaccggcttc cggtcactgg tactttacac
     4621 tcaaagacga caccgcccag gtacgctgcg cgatgttccg caacagcaac cgccgggtga
     4681 ccttccgccc acagcatggg caacaagttt tagttcgcgc caatattacg ctctacgagc
     4741 cgcgcggcga ctaccagata atcgttgaga gtatgcagcc ggccggtgaa gggctgctgc
     4801 aacagaagta cgaacagctc aaagcgaagt tgcaggctga aggattgttc gatctgcaat
     4861 acaaaaaacc acttccctcc cctgcgcatt gcgttggtgt gatcacctca aaaaccggtg
     4921 ctgcgctaca tgatattttg catgtgttaa aacgtcgcga tccttctctg ccggtgatca
     4981 tctaccccac cgccgttcag ggcgatgacg cgccggggca aattgttcgc gccattgaac
     5041 tggcgaatca gtgcaatgag tgcgacgtgt tgatcgttgg gcgcggcggc ggttcgctgg
     5101 aagatttatg gagttttaac gacgaacgcg tagcgcgggc gatttttgcc agccgcattc
     5161 cggtcgtcag cgccgtcggg catgagacgg atgtgaccat tgccgatttt gttgccgatc
     5221 tgcgtgcacc aacaccgtcg gctgccgccg aagtggtgag ccgtaatcag caagagttac
     5281 tgcgccaggt gcaatcgacc cgtcaacggc tggagatggc gatggattat tatctcgcca
     5341 accgcacacg tcgctttacg caaattcatc accgattaca gcaacagcat ccgcagctcc
     5401 ggctggcacg ccagcaaacc atgcttgaac gcctgcaaaa acggatgagc tttgcgctgg
     5461 aaaatcaact taagcgtgcc gggcaacagc agcagcgatt aacacagcgg ctgaatcagc
     5521 aaaatccaca gccgaagatt catcgcgcgc aaacgcgcat tcagcaactg gaatatcgtt
     5581 tagcagaaac cctgcgcgca cagcttagcg ccacgcgtga acgtttcggt aatgcagtaa
     5641 cgcacctcga agccgtaagc ccactgtcaa cgctggcgcg tggatacagc gttactactg
     5701 ctactgacgg caaggtactg aaaaaagtga agcaggttaa agcgggtgaa atgctaacca
     5761 cacgtctgga agacggctgg gtagaaagtg aagtaaaaaa catccagccg gtaaaaaaat
     5821 cgcgtaaaaa agtgcattaa gccagcacga attcaacgcg ttttttcgat atcaagccat
     5881 gcccgtgctg acagaagtaa tctaccgcac cgcaggcttt taacacctgc aacggttgat
     5941 ggcagtccgg gcaaagagct ttcatttcaa taactttccc gcaggatggg caatacgcat
     6001 ggccgttatc ctgatcaagc acatgctgac attgtggaca atgcagttcc atatgaattc
     6061 ctccggtagc cacccctcta cattcataga gggaatggca gataaaatac ttacggataa
     6121 ttatttattt ttcttgatgt gcttcatcag acgcttacgt ttacgcatct gggttggcgt
     6181 cagggtgtta cgcttgttcg catacgggtt ttccccttct ttgaactgaa tacgaatcgg
     6241 cgatcccatt acgtccagcg atttgcggaa gtagttcatc aagtaacgct tgtaggaatc
     6301 aggcaggtct ttcacctgat taccgtgaat caccacaatc ggcggattat aaccaccggc
     6361 gtgtgcatat ttcagcttca cacgacgacc gcgtaccagc ggcggttggt gatcttcaac
     6421 agccatcgtc atgatgcgcg tcagcataga ggtccccaca cgacgggtgg agctgtcata
     6481 cgcttcacgt actgattcaa acaagttacc aacaccactg ccgtgcaagg cagagataaa
     6541 gtgcacacga gcaaaatcga taaagcccag acggaagtcc agcgtttctt tcacctgctc
     6601 tttcacttcc tgactcaggc catcccactt attcaccaca atgacaagtg agcgcccact
     6661 attgagaata aagcccagca gcgagagatc ctgatcggaa ataccttcgc gcgcatcaat
     6721 cactaacatc accacgttgg cgtcttcaat ggcctgcaac gttttgatta cggagaattt
     6781 ctctacagca tcggtgattt tgccgcgttt acgtacgcca gcggtgtcaa tgagcacata
     6841 ctcacgtcca tcgcgttcca ttggaatgta gatactgtca cgcgtcgtac caggcatgtc
     6901 ataaacaaca acgcgctctt caccaagaat acggttagtg agtgtagact tacctacgtt
     6961 cggacgaccc acaatcgcca gtttgatcgg cagactttgc gggtcgaagt cgtcttcctc
     7021 ttcttcttcg ccgttctctt ccgcttcaaa ttgcgcccag tattcagcgt cttcgtcgac
     7081 ttcctcttgc ggtgcgagat cttccatcca cggcagtagc acatgctcca gcagacttaa
     7141 tacgccacga ccgtgagacg cggcgatcgg gtagatttca cctaaaccaa gcgagtagaa
     7201 atcaaccact gcctgatcgg gatccagacc gtcagttttg tttgccacca ggaaggtcgg
     7261 tttttcacgg gagcgcagat gtttggcaat cgcttcatct gccggcatca ggcccgcgcg
     7321 cgcatccacc ataaacagta cgacgtccgc ttcttcaatc gccagcagcg actgttccgc
     7381 catgcgggtt tctacaccgt cttctgtgcc atcaatcccg ccggtatcaa tacagataaa
     7441 ctcacggcct tcaatttccg cacgaccgta cttacggtca cgagtcagac ccgggaaatc
     7501 cgcaaccagc gcatctcggg tgcgagttag acggttaaat aacgtggatt ttcctacgtt
     7561 agggcgcccg acaagcgcga ccacaggtac catgtttaaa gcctcatttt tataaatcat
     7621 cagacaacgc acgctatatt cgcgtcgttg ttaaaaacag gaaaacggcc cctgtccagg
     7681 agccgttttc aaagtgaacg acagagacga ttaacgtgta atagagtaca cggttccgtc
     7741 ttttgcctgg atcagcagtt tgccgtcagc ggcaaccggt tcagtctgga aaccggaact
     7801 atcaactttt tgctgggcaa cgaaacgacc atcttcgacg ttaatccagt gcaaataacc
     7861 ttcactgtca ccgaccacca ggttgccatt atacagcacc ggagaagtca gcaggcgatg
     7921 cagcagatcg ctttgtgtcc acagcgtaac gccgccatca atggtcaacg ccatcacccg
     7981 gtcattttga tcgaccagat agatgcgatt gccgtcgacg atgaaatcat tcaccgaacc
     8041 cagttcgcgt ttccacataa tctgaccact gcgcagatca agcgccgtca ggttaccatt
     8101 ataggccagc gcgaaaacaa cgccgttaac aacggcggga gtcgtgtcaa catcgctcag
     8161 acggtcaatt tcggtagaac cggtcgcttg ggaaatacgc tgctgccaaa tcatctggcc
     8221 ctgttccatc agcactgcgc tgacgcgacc attatcgccc cccacgacgg ccgcaccaaa
     8281 agccgttgcc ggcgcagact cgccacgcaa agagagcgaa ggcatatcga ggttaactgt
     8341 ccatttgaca gcgccgtcag cttcgttcag cgcttgtaac tgaccgttac tggtgtggat
     8401 taacaccaga ccgtcgctga ccaccgggcg cgaaagtgct tcacccgcga ctttagtttg
     8461 ccatgccaca gtaccatcgc tggtattcag cgcgtaaacc tgcgcctttt cgctgccaat
     8521 gtagacatgt ccaccagaca cggtcacacc gccagaaagt aatgcaggct ctttagagaa
     8581 ccagccatct ttctcggcca ggctgacaga ccagatttct ttgccatcat ccgcattcag
     8641 cgcttttact aaaccagcgc ggtccgctgc atagacaacg ttgtccgcca gtgccggatg
     8701 aagattggaa tagaagttgc caatgccgct accaacggaa gtgctccacg ccgtggtcgg
     8761 cgtaaactgg ttttcaacgg ttggcaatgg ggacatcttt accacatctt cttcgctgtt
     8821 aaacagcgaa cagccgctta aaagggtaac ggaaagcagt cctggcagca gtaatttacg
     8881 caattgcatc gggtccctct cagatggaca aattattaat tttcatctgc atcatttcgc
     8941 tcagtgccgg agtaacatcg cttttcacgc ctgcttccca tgcactacgc gcaccttgct
     9001 tatcaccttt gctcagcaat gcttcaccac gcaggtcggc aacaatggca gcccaccctt
     9061 cacctttgat ggtatcaagg gttttcagcg cggcatcagc ctgcttgagc tgtacctgaa
     9121 cgcgagcaag acgcagattt atcacggctt tgagattttc atcgctcgtg tctgccagcc
     9181 cctgttgtaa ctgggcggca gctttctcta gttcattttt gtcaacaaat tgctgcgcaa
     9241 gttccaaaga agccagcgca ccataagtat ttttattttc agcagcaaat ttttccgccg
     9301 ccgggatgct atccggtttg ccttcgctca ctgcggtaac cgcattttga taggcaagag
     9361 aagcggagcg tgcagaatca acctgatggc tgttccagta gcgccagcca atcagtgcgc
     9421 caacgcccaa aatcacccca acagccagtg ctttgccatt ttcagcaaaa aagcgtttaa
     9481 tcgcttctac ctggtcgttt tcgttctcgt aaatttccac gctgtccttc tccttcctta
     9541 acccagtaac gtgcgcaaat gcgcggctac gctatcctgc gcaactgccg tttgctcacc
     9601 agagcgcaaa tccttcacta ctgctgtgcc gttagccact tcagactcac ccagcaccac
     9661 agcaacgcgg gcaccccatt tatcagcacg ggcaaactgt ttcttaaagt tgccgccgcc
     9721 gtggttggtc atcaatttca cgcccggtaa ttcatcacgc agacgctcag ctaatgccat
     9781 agccgcagat tgtgtatcag cacctgaagc caccaggtat atatcgacaa caggatcggc
     9841 tttaaattcc ggattaacgg cctgtactaa caatacaaga cgttcgaggc ccatcgcaaa
     9901 accgacagcc ggtgttgcac ggccgcccag ttgttccaca agaccgtcat aacgaccgcc
     9961 tgcacacacg gtgccctggg agccgagact gttagtcacc cactcgaaaa cggtacggtt
    10021 gtagtaatcc agaccacgca ccagacgctg gtttacggtg taagcgatcc ccgcgctctc
    10081 aagcagtttg cacagaccgg caaaatgctc acgagattcc tcgtccagat aatcacctaa
    10141 tgccggagcg tcgttgagaa gcgcctgcac ttccggattt tttgaatcca gcacgcgcag
    10201 cgggttagtg tacatgcggc gtttgcagtc ttcgtccagc ttttctttat gctgctcaag
    10261 gaatgccacc agcgcatcgc ggtaattggc gcgtgcttcc agcgaaccga tagaattcag
    10321 ctcaagagtt acgtgttcgg aaatacccag cgcgcgccac cagcgggcgg tgagcataat
    10381 cagttcagcg tcgatatccg gaccttgcag accgaaaact tcgcagccca actgatggaa
    10441 ctgacgataa cgccctttct gcggacgctc gtgacggaac atcggcccga tataccacag
    10501 acgctgttcc tgattgtaca gaagaccatg ctcgatgccg gcgcgtacac agcccgccgt
    10561 cccttcaggg cgcagagtca ggctgtcgcc attgcgatcc tcaaaggtgt acatctcttt
    10621 ttcaaccacg tcggtgactt caccaatcgc acgtttgaat agcggggtct gctctacaat
    10681 cggcaagcgg atttcactgt aaccgtagct gccgagcacg tttttcagtg tgccttcaat
    10741 gcgctgccag atggccgttt cgccaggcag gtaatcgttc atgccgcgaa tggcttgaat
    10801 gttttttgcc acgtttattc tctttctgaa tataaaaatg aaccctcaac gcttccctca
    10861 atgtttcggg agccatgcgg gttcaatcat acacgggaag cgaggcgctt cccatcacgt
    10921 tattattttt caacctgctg aacgtcaatt cgacgcgctt cgtccagctg actggctttc
    10981 gcacgaatgc gtgcctccag ctggtcgatc atatcgttgt tgtccagacg gtctttgcgc
    11041 acgccatctt catagagacc gcttttcttg ttgccgccgg tgacgccgag tgtagaaacc
    11101 agcgcctcac ccgggccatt caccacgcag ccgataatcg aaacgtccat cggagtgatg
    11161 atatcttcca ggcgttgctc cagcgcgtta actgtaccga taacatcaaa ttcctgacgc
    11221 gaacaggtcg gacaggcgat gaagttgata cctcgcgaac ggatacgaag cgatttcaaa
    11281 atatcgaaac cgactttgat ctcttcgacc ggatcggccg ccagtgatac gcgcagcgtg
    11341 tcgccgatgc cttcagacag cagcagacct aaaccaatgg cggattttac tgccccgctg
    11401 cgcgcgccac ccgcttcggt gatccccaga tgcagcggct gatcgatttg ttttgccagc
    11461 aaacgataag actcaacagc gaggaagacg tcagacgctt tcacgctgac tttgaactga
    11521 tcgaagttca ggcgatcgag atgatcaaca tggcgcatgg cggattccag caacgcctgc
    11581 ggcgtcggtt cgccatactt ttcttgcaga tctttttcca gcgatccggc gttaacgcca
    11641 atacggatcg gaatgttttt atcgcgcgca cagtcaacca ccatgcgaat acgctcttca
    11701 ttaccgatat tgccagggtt aatacgcaga caatcgacgc cgtattccgc tactttcagc
    11761 gcaatgcgat agtcgaagtg gatgtcagcc accagcggca cgttaacctg ctgtttgatg
    11821 agtttgaacg cttctgccgc gtccatcgtc ggtacggaga cacggacgat atcagcgcca
    11881 acgcgttcca gcgccttgat ttgattgacc gttgcttcga catctgtcgt acgcgtattg
    11941 gtcatggact gtacggcgat gggagcacca tcgccaatcg gcacattccc aacgtaaata
    12001 cgtgttgatt ttctacgttg aattggagcc tggttatgca tgaaaaatct cccgcgttac
    12061 ccgtctgtta ctgcgccggt gattgttcgg cattgagggt cagacgcgca acctggttag
    12121 ttctgataaa acgactcaga tcgacaggtt tcccttgata ctggatctgt actgcggctg
    12181 gcgcaccaat tttcagtttg tacggtgcct ggccggttaa gttcaaatta ccgtctttac
    12241 gctgcatacc gctaaacaat tttttaccgg tagcatcagt gacctccagc cagcaatcgg
    12301 cagtaaagtt catcaccagc gcattcggat cagccaccgg cgtggtcacg ccagcctgat
    12361 cggttggcaa cggcgcagca ccatctggcg ttgttgctgc cgttggtgcc ggggtcgcgg
    12421 cggtatcaac atttgcctgc gaaggcgaaa caaccgcatt ctgttgcgga tcaacagctg
    12481 gtgctggcgc agttacgaca ggtgtttgcg tgttggttgc ggtagtatcc acagacgctg
    12541 gcggcgtgga cgttgtagcc gggtctgtag ttgtcgacgt atttaacgga acactctgcc
    12601 cctgctcgct attactgctc agttccgccg aagattgatc ggccatagtg gtgatctctt
    12661 cctgctgagc tttgtggtct tgccaccacc aggcaccgct caggccgata accacaaaca
    12721 acaccagcca ggtgaaggtc atcagccagc cgtcgcgttt tttgcggcgt ttaccgaggg
    12781 aaaaactctg catcggcgca acttttgcag cccgtagtgg agcctgcttt tccagccctg
    12841 gcagcagttc ttcttctgga atatgtacca gacgcgcata agagcggata tatccgcgca
    12901 ggaatgttga agcaagatcg gcgggtgcct tatcttcttc aatgtcgcgt accgtggaaa
    12961 ccttcaggca aagtcgctcg gcaacggcct gctgactaag tcctagttgt tcgcgagcat
    13021 tacgcaggcg agcgccggta gtaagtgctt cattttggtc gtgcgtggct tcagtattca
    13081 ttcgctgcag gtacgtttaa atgagaattg agatgccggt gaaccatcat gctcacccac
    13141 accgcgaaac atccgttaag ttaaccgtta tcatacagta taagaccgtc tgtgtgctcg
    13201 tgacaaagcc cacattcact aaggctaact tactgttgca tcgttacata ctgccttaaa
    13261 gtcagcaaaa acgcaccgtt aatattgacc agacaattgc aacttaatac ttcattcata
    13321 aaacattacg ccacggtaca taaagtaacc gtggcgtaat ggctatcaga ccgctttaat
    13381 gtcgatggct tcaccctgca tccgtttacg cagggtacgt ttcgtacggt cgataacatc
    13441 gcccgccaac tgaccacagg cagcatcgat atcatcacca cgcgttttac gcacaatagt
    13501 ggtgaaaccg tagctcatca gcacttttga gaaacggtcg atacggctgt tcgagctgcg
    13561 tccatacggc gcagccggga acgggttcca cgggatcagg ttgatcttac acggcgtatc
    13621 tttcagcagt tccgccagtt ggtgcgcgtg ttcagtgccg tcgttaacgt ggtcaagcat
    13681 cacgtattca atagtgactc ggccctgatt ggcgttggat ttctccagat aacggcgcac
    13741 cgcagcaagg aacgtttcga tattgtactt tttgttgatc ggcacaattt cgtcacgaat
    13801 ttcgtcgttc ggcgcgtgca gggaaattgc cagcgcaaca tcgatcatat cgcccagttt
    13861 atccagcgct ggaactacac cggaagtgga aagcgtcacg cgacgtttag acagaccaaa
    13921 accgaaatca tcaagcatga tttccatcgc cggaacgacg ttgttcaggt tgagcagcgg
    13981 ctcgcccatg cccatcatca ctacgttagt gattgggcgc tgaccggtga cttttgctgc
    14041 gccgacgatt ttcgccgcac gccacacctg gccaataatt tccgataccc gcaggttgcg
    14101 gttaaagccc tgctgggcgg tggaacagaa tttacactcc agcgcacacc ccacctgcga
    14161 agagacgcag agcgtagcgc ggtcgtcttc cgggatgtac accgtttcga cacgctggtc
    14221 acccacggca atcgcccatt taatggtgcc gtcagatgaa cgctgttctt caaccacttc
    14281 cggtgcgcgg atctccgcca cctctttcag tttgccgcgc aacactttgt tgatgtcggt
    14341 catctcatca aaattgtcgc agcaatagtg atacatccac ttcatcacct gatcggcgcg
    14401 gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc tgctgacgat tgagatccag
    14461 caggttgatt tttccatctt tggtggtgac gttttcaggt gtgactaatt gttcagacat
    14521 atgctattcc ggcctcgtta ttacacgtta tggcccctgg agggttgaaa aaagaaacgc
    14581 cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac aaattctggc gcacggatgc
    14641 cacgtttgca cgcggcattt acgaaattat taacgggtgc gcgggcacac ttcgccttcg
    14701 ccgaagaaat aagcgatttc gcgagcggca gattcgacgg aatcagaacc gtgggtaccg
    14761 ttttcggtca ggctgtcagc gtaatcagcg cgcagagtac cggccagtgc gtttgccgga
    14821 ttggtcgcgc ccagcagatc gcggtgacgc tgaacggcgt tttcaccttc cagcacggaa
    14881 accacgatcg ggccagaggt catgaattca accagaccat caaagaacgg ttttccatcg
    14941 tgttcagcat aaaagccacg tgcctgttca acggtcaggt gcagcatttt ggtgccgaca
    15001 attttgaacc ctgcagcttc aaagcgcgca aagatattac caatgacgtt ttttgctacc
    15061 gcgttcggtt tgataatgga aaaagtacgt tcaatagcca tttttacctc tgtaaattgt
    15121 tctgttgttg tctgtaccag cgtacgagat ggcgcggatt ataatgagca acagagccgt
    15181 tgactattga tgaaggtaac atttttttaa aataaaatga gttttagcaa caactcgact
    15241 tttatggtgt tctcatccca taacgatgac gttggcaata aagacaattt tgcgcatttt
    15301 gctgaaaagt aagaaaggat ttttgacagg ttttacagac tttgcgatta gcatgaagat
    15361 tcacaatttt tgccggtaga atcgtcaatt caacggtcat tgcctgatga aaacaaacag
    15421 caatacaatt ttggcatccg ttacacaagg catcatttat ttgtaaatgg ccttcgttaa
    15481 gcgaaaagac ctgttgctcg caggcccgcc agcatgcacc gcaaagctga cacttttgag
    15541 gatccatcat cgggcgagca tcactaatct ggggccataa ataatgtaac cgacgtaatc
    15601 ctggttgtat ttgtgtcatc tgaacatccg cacgctgcac atggaacaaa cgacgccggg
    15661 aaacattcac ctcactggta tgtggagata ccacagacca gccttcctcg ccatattgcc
    15721 gtaagcattc gtttaacagc aataatgcgc gctcccatgc cttcgcctga ctcagatcaa
    15781 tcgcgatcgc tgtgatcccc tgctctttat gccatagcaa aagttcttcc agcgtcggcg
    15841 caatagtggt gaaaggtgcg accagggtgt gattgcaaaa ataacgtaaa ggggaattaa
    15901 cgccacgaat agcctcttgc ggacaaataa actggcaaac cgcacaatca atacattgtt
    15961 ccgcgtcgac cgctatgccc tcttctgtca gcgttagcgc ctgtgccggg caagcctcga
    16021 cacaggcatg acatgttgca aaacgaaagc gttggcgcgc gcattccgcg ccaatcgtta
    16081 tgttatgact taacattagc cgatactaaa aaagacaaaa cgcagcataa cttcactccc
    16141 cactagcatt acgctaccga ataacgccgc cgggatattc tttttacaac caccgacagc
    16201 aaagaacatc atcccaatga cggaaattcc ccagcttagt gtcagtatgg gttttaactg
    16261 tcccagcatc gctaatggcg catgtggcaa tgtggctatc tgcatcgcat ttgccgttaa
    16321 atcccctatc cacaacggct gaaccaacaa acgcagcaga accaggagag cgatcaccaa
    16381 cacgcatcca ttacgcaggg catcaatctg cggcaaaatt ccgctaatac gaaacactgc
    16441 taccagcgct gaaccgagaa ttcccactgt acctaagaac ataatcagcg tgttgtaatg
    16501 ctgccaggtg ataatcgacg tatggatgta aacttgcgcc atgcagaaaa catcaacaat
    16561 cccgaccact gccgctagca taagcaggag ttgccagccg cctttgcgta acatgaccag
    16621 taatgtggat aaacccaata gcgccagata cagcgcacca aagataattt cccgactcag
    16681 ccatgaactg gaaaagtggc tcagcgcatg catcgcatta agtggataac ccaggtgtaa
    16741 ggtagaaatc agtaacccta cggcacctgc caaaaaagcc aacagtaatg gcggccgcat
    16801 aaccgggcgg gcgctggagg tgattaaaat atgacgatga agaaacgcca gccacaacgt
    16861 cataccgacc gatgcctgta acaacagcgt aaaagccatt aacggtaatt catgcatgat
    16921 gtgacgcctc cttttccgcg ccctgatgcg gtttaatcac cagatttggc tgcgtaatcg
    16981 acgaatcagg taaacctttc acatcactga catcgccata cttttcacgc aactcatcaa
    17041 tagggccgaa cttaatcgcc cccaacgggc acgttgccac gcaaatgggt tgttctcctt
    17101 tagcttgcag atcaatacag aaatcgcatt tcgacatctg cccggtctgc tcattcattt
    17161 gtggtgcgcc gtaaggacag gaccaggcgc agtaaccgca accgacgcac ttatcagtat
    17221 tcacgcgcac aataccgtct ccgggacgct tatgcatcgc catcgtcggg caatttttgg
    17281 tacagatcgg atccgcgcaa tggttacagg aaacagaaag ggtataagca aagacattat
    17341 gactcacacc gccctctcca gtggcgatga agccacctcc gttcacttca tacacgcggc
    17401 gaaaacgtcg cccaacctca aggttgtttt tgtctttaca ggccacctga cacgctttac
    17461 agcccgaaca acgcgaagag tcaataaaga accctaattg tttgtcgctg acagatgcaa
    17521 attctttaaa ttggctcatg ctttctcaac ctccaccagc attgtttgat gcgaattccc
    17581 tttcgccagc gccgtaatgc gggtcgaact cagtacattg gcacagcctc cgcgatcgat
    17641 accttgtgca tccggttgcc accatgcccc cgcctgcata gcaactacgc cagggatgat
    17701 acggggggtt atttccaccg ggataatgct cacaccacga tcgttataaa tttttacgct
    17761 atctccttcg ctaatcccgc gctgttttgc atcttgcgga ttcagccaga gtttttgcgt
    17821 ctgaacttct tgcagccacg ggttagcata ctgggtggaa ttagcacggt ttttcccttt
    17881 ccaggtgatg agctgcaacg ggtatttcgc cgtcagttta tcttctggcc cttcaaaggc
    17941 agggacatag tgcgaaagcg cgggaatttc tggatcttgc atatcaaaaa gacgcttaga
    18001 gaagatctca attttgccgg aaggcgtcgg gaaaggatta ttctgcggat cgcgaatatt
    18061 tgcttcgaaa gcgatgtgcg gcgcgctttt gaaaagatgt cgacgtgttt tttgcagggt
    18121 tgcgaagtcc ggcaaatttt catccggcat cgccagacga gtttgctccc agatatgctc
    18181 gatccattgc ttctcatcac gtccttgact gaactcggct tcgacaccca gttttttcgc
    18241 cacgtcacgc aaccagtcat aatcggtgcg acgttcaaaa tccggctcaa tcggtttttc
    18301 tgacagaata agatagctcg cagttcccca tgtttcgccg atattccagc gttccataaa
    18361 gctggtttcc ggcagcaaca catcggcata tttcgcgctc ggagtcatga acagatcgct
    18421 gagtacgatg aattctattt tagattcgtc ttcaaggagc ttcgcggcct ggtggacgtc
    18481 cgggttctgg ttcgccagat aattacctgc cagtgagaac agcaggcgga tattactgtc
    18541 gagtttatcg acacctttaa gcccatcttg cggcgtaact ttagaagcat catccgccgc
    18601 ctgcatccag ttcataatcg agattttcgc ttgtaccgga ttttctggca tatctggtcc
    18661 aacgcagaat ttacgattag aactaccgcc gtaacctgcc gcccagccac ctttgatgcc
    18721 gacgttaccg gtaatcgagg ccagtaaagt tgaacctcgg gcggtgcgtt cgccgcaaat
    18781 atgtcgctgt ggcccccatc cctgaatcaa cgctgcgggt tttgtagtgg cgtaatcgcg
    18841 tgccagttga cgaatggatt gtgctggaac gtgggtaatt ttttctgccc attccggcgt
    18901 tttgtgtatc ccatcttttg cgccaaaaag gtaggcaacc aacgattcgt tggctggtac
    18961 gccttccggc atggagtttt catcaaaacc gagagtatat gtgtcgataa atgtcttatc
    19021 gtggagattc tcgctgataa tgacgtacat catcgcgtcc atcaacgcat tgtccgtggt
    19081 cggcaacagc gggatccact gatcggcaag tgaagatacg gtgtcggaat agcgcggatc
    19141 caccacgata aaacgtgtac cgttttgctt cattttttga aaatagtaat tggtgtgacc
    19201 gaatatggtt tccgttgggt tatgccccca gagaatcacc agtttggtat ccagaagagt
    19261 atccatcgag ttaccgctcg cggccacgcc atacgtgtaa ggcgtg
//
LOCUS       DAZKIF010000095        19091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZKIF010000095 DAZKIF010000000
VERSION     DAZKIF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.095"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(160..1809)
                     /gene="pgi"
                     /locus_tag="ACHOTW_003291"
     CDS             complement(160..1809)
                     /gene="pgi"
                     /locus_tag="ACHOTW_003291"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9845341.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2046..2242
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2334..3683
                     /gene="lysC"
                     /locus_tag="ACHOTW_003292"
     CDS             2334..3683
                     /gene="lysC"
                     /locus_tag="ACHOTW_003292"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP9845342.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3936..4208
                     /locus_tag="ACHOTW_003293"
     CDS             3936..4208
                     /locus_tag="ACHOTW_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP9845343.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4209..5081)
                     /gene="rluF"
                     /locus_tag="ACHOTW_003294"
     CDS             complement(4209..5081)
                     /gene="rluF"
                     /locus_tag="ACHOTW_003294"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP9845344.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5429..6376
                     /locus_tag="ACHOTW_003295"
     CDS             5429..6376
                     /locus_tag="ACHOTW_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFP9845345.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6446..7252
                     /locus_tag="ACHOTW_003296"
     CDS             6446..7252
                     /locus_tag="ACHOTW_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9845346.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7262..7669
                     /locus_tag="ACHOTW_003297"
     CDS             7262..7669
                     /locus_tag="ACHOTW_003297"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFP9845347.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7669..8163
                     /locus_tag="ACHOTW_003298"
     CDS             7669..8163
                     /locus_tag="ACHOTW_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9845348.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            8229..9026
                     /locus_tag="ACHOTW_003299"
     CDS             8229..9026
                     /locus_tag="ACHOTW_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9845349.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            9037..9861
                     /locus_tag="ACHOTW_003300"
     CDS             9037..9861
                     /locus_tag="ACHOTW_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9845350.1"
                     /translation="MEQRKITRGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9913..11142
                     /gene="sorE"
                     /locus_tag="ACHOTW_003301"
     CDS             9913..11142
                     /gene="sorE"
                     /locus_tag="ACHOTW_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFP9845351.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11525..12214
                     /gene="pepE"
                     /locus_tag="ACHOTW_003302"
     CDS             11525..12214
                     /gene="pepE"
                     /locus_tag="ACHOTW_003302"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP9845352.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12305..13936)
                     /locus_tag="ACHOTW_003303"
     CDS             complement(12305..13936)
                     /locus_tag="ACHOTW_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP9845353.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14156..17839)
                     /gene="metH"
                     /locus_tag="ACHOTW_003304"
     CDS             complement(14156..17839)
                     /gene="metH"
                     /locus_tag="ACHOTW_003304"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9845354.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18039..18863
                     /gene="iclR"
                     /locus_tag="ACHOTW_003305"
     CDS             18039..18863
                     /gene="iclR"
                     /locus_tag="ACHOTW_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP9845355.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4497 a         4977 c         5022 g         4595 t
ORIGIN      
        1 attaatcgtg ctatgcatta attgccgaat acgatgtaca tatcggcatc gacctgtagg
       61 cctgataaga cgcgtaaagc gtcgcatcag gcaccagttg ccggatgcgg cgtaaacgcc
      121 ttatccggcc tacatagcgg catcaggcat gaacgatgat taaccgcgcc acgctttata
      181 gcggttaatc agaccattgg tcgagctatc gtggctgctg atttctttat catctttcag
      241 ctctggcaga atacggttcg ccagctgttt acccagttcc acgccccact ggtcgaaggt
      301 gaagatgttc aggatcacgc cctgagtaaa gattttgtgc tcatacagcg caatcaacgc
      361 acccaggctg aacggagtga tttcgcgcaa caggatagag ttggtcgggc ggttaccttc
      421 gaacactttg aacggcacca cgtagtcaag cgttgccgga tctttaccct gatcgcgata
      481 ttcctgctca accacttcgc gggatttacc aaacgccagc gcttcggtct gggcgaagaa
      541 gttagacagc agtttctggt ggtgatcaga gagcgggtta tgggtgatag ccggagcgat
      601 gaaatcgcac ggtaccattt tggttccctg gtggatcagc tggtagaacg cgtgctgacc
      661 gttagtgcct ggttcacccc agataatcgg gccagtctgg taatccacaa cgttaccgtt
      721 acggtcaaca tacttaccgt tggactccat attgccctgc tggaagtacg ccgcgaaacg
      781 gtgcatatac tggtcatacg gcagaatcgc ttcagtttcc gcaccaaaga aattgttgta
      841 ccagatgcca atcagcgcca acagtacagg caggtttttc tcggcaggcg tggtggagaa
      901 atgcttgtcc atcgcgtgtg cgccagaaag cagttcaacg aagttatcaa agccgatgga
      961 gagaacaatc gacaggccaa tcgctgacca caaagagtaa cgaccgccga cccagtccca
     1021 gaactcgaac atgttggcag tatcaatacc aaactcgcca acggctttgg cattggttga
     1081 gagcgccgca aagtgtttcg caacgtgttt ttcatcacct gccgctttca ggaaccagtc
     1141 acgcgcgcta tgggcgttgg tcatggtttc ctgagtggtg aaggttttag atgctaccag
     1201 gaacagcgtg gtttccgggt ttactttttt cagcacttca gcgatgtgag tcccatcgac
     1261 gttagaaaca aagtgcatgt tcaggtgatt tttgtacgga cgcagtgctt cggtcaccat
     1321 gtacgggccg aggtcagaac cgccgatccc gatgttcact acgtcagtga ttgctttgcc
     1381 ggtataaccc ttccactcac cggagataat cgcttccgag aaggttttca tcttctccag
     1441 caccgcgttg acttctggca taacatcttt gccatcaacc agaatcgggg tattgctacg
     1501 attacgcagc gctacgtgca gcacggcgcg gttttcagtg cggttgatct tctcgccaga
     1561 gaacatcgac ttaatcgcgc ccgccagatc gcactctttc gccagatctt gtaatttcgc
     1621 cagcgtctct tcagtgatgc ggtttttgga gtaatccacc agcatctgat cgtcgaaggt
     1681 tgcggagaac ttagaaaaac gatcgccgtc tttagcaaaa agatcggcga tcgtaacgtc
     1741 tttcatttca tcgaagtgtt tctgtagtgc ctgccaggca gcggtctgcg ttggattgat
     1801 gtttttcatt agcaatactc ttctgatttt gagaattgtg actttggaag attgtagcgc
     1861 cagtcacaga aaaatgtgat ggttttagtg ccgttagcgt aatgttgagt gtaaaccctt
     1921 agcgcagtga agcatttatt agctgaacta ctgaccgcca ggagtggatg aaaaatccgc
     1981 atgaccccgt cgttgacaac cgccccgctc accctttatt tataaatgta ctacctgcgc
     2041 tagcgcaggc cagaagaggc gcgttgccca agtaacggtg ttggaggagc cagtcctgtg
     2101 ataacacctg agggggtgca tcgccgaggt gattgaacgg ctggccacgt tcatcatcgg
     2161 ctacaggggc tgaatcccct gggttgtcac cagaagcgtt cgcagtcggg cgtttcgcaa
     2221 gtggtggagc acttctgggt gaaaatagta gcgaagtatc gctctgcgcc cacccgtctt
     2281 ccgctcttcc cttgtgccaa ggctgaaaat ggatcccctg acacgaggta gttatgtctg
     2341 aaattgttgt ctccaaattt ggcggtacca gcgtagctga ttttgacgcc atgaaccgca
     2401 gcgctgatat tgtgctttct gatgccaacg tgcgtttagt tgtcctctcg gcttctgctg
     2461 gtatcactaa tctgctggtc gctttagcgg aaggactgga acctggcgag cgattcgaaa
     2521 aactcgacgc tattcgcaac atccagtttg ccattctgga acgtctgcgt tacccgaacg
     2581 ttatccgtga agagattgaa cgtctgctgg agaacattac tgttctggca gaagcggcgg
     2641 cgctggcaac gtctccggcg ctgacagatg aactggtcag ccacggcgag ctgatgtcga
     2701 ccctgctgtt tgtcgagatc ctgcgcgaac gcgatgttca ggcacagtgg tttgatgtac
     2761 gtaaagtgat gcgtaccaac gaccgatttg gtcgtgcaga gccagatgta gccgcgctgg
     2821 cggaactggc cgcgctgcag ctgctcccac gtctcaatga aggcttagtg atcacccagg
     2881 gatttatcgg tagcgaaaat aaaggtcgta caacgacgct tggccgtgga ggcagcgatt
     2941 atacggcagc cttgctggcg gaggctttac acgcatctcg tgttgatatc tggaccgacg
     3001 tcccgggcat ctacaccacc gatccacgcg tagtttccgc agcaaaacgc attgatgaaa
     3061 tcgcgtttgc cgaagcggca gagatggcaa cttttggtgc aaaagtactg catccggcaa
     3121 cgttgctacc cgcagtacgc agcgatatcc cagtctttgt cggctccagc aaagacccac
     3181 gcgcaggtgg tacgctggtg tgcaataaaa ctgaaaatcc gccgctgttc cgcgcgctgg
     3241 cgcttcgtcg caatcagact ctgctcactt tgcacagcct gaatatgctg cattctcgcg
     3301 gtttcctcgc ggaagttttc ggcatcctcg cgcggcataa tatttcggta gacttaatca
     3361 ccacgtcaga agtgagcgtg gcattaaccc ttgataccac cggttcaacc tccactggcg
     3421 atacgttgct gacgcaatct ctgctgatgg agctttccgc actgtgccgg gtggaggtgg
     3481 aagaaggtct ggcgctggtc gcgttgattg gcaatgacct gtcaaaagcc tgcggcgttg
     3541 gcaaagaggt attcggcgta ctggaaccgt tcaacattcg catgatttgt tacggcgcat
     3601 ccagccataa cctgtgcttc ctggtgcccg gcgaagatgc cgagcaggtg gtgcaaaaac
     3661 tgcatagtaa tttgtttgag taaatactgt atggcctgga agctatattt cgggccgtat
     3721 tgattttctt gtcactatgc tcatcaataa acgagcctgt actctgttaa ccagcgtctt
     3781 tatcggagaa taattgcctt taattttttt atctgcatct ctaattaatt atcgaaagag
     3841 ataaatagtt aagagaaggc aaaatgaata ttatcagttc tgctcgcaaa cgaattcccg
     3901 tacactgaac tctctggctc actgagggca ccatcatggc actcccccgt attacccaaa
     3961 aagagatgac tgaacgcgag cagcgtgaac ttaaaacact gctggatcgg gcgcggattg
     4021 cgcatggtcg cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata
     4081 aattaatggt cgagcgtgag gctgaagcga aaaaagcccg ccagttaaag aaaaagcagg
     4141 cttataaacc ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca
     4201 ggcgctgatt agcgcccctt cttttttcgc cccggcgagg taaaacgctt accgttggac
     4261 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
     4321 gctgttttcg gtttggcctt cgctttaggt tttgcctcgg aagaggaatt ttcgatgagc
     4381 ttaaagaggt cgattaactc atcgtcggtt aaatcgcgcc actcccccag cggaatgccg
     4441 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
     4501 tcgcacatgc gccggatctg acggttcagc ccctgcacca gggtaatgcg gaagacaaac
     4561 ggcgcttctt ttttgacttt gcacttttta gtcaccgtcc cgaggatcgg cacccccgcc
     4621 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
     4681 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
     4741 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
     4801 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat gcctacgggc
     4861 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
     4921 actttcacaa cgtcgccggg tttcacctga tcgccaatgg tggctcgctt gccattaagg
     4981 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
     5041 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
     5101 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
     5161 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacaatgc aaatttgcgt
     5221 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg ataactactg
     5281 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
     5341 cgctttgaaa ggccatcatg atggggttat atcagatcaa gcggtgagaa agcgtatttg
     5401 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
     5461 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
     5521 gggtatttac cgcaccaaca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
     5581 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
     5641 aaagtttggc ctgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
     5701 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
     5761 tattgtcggt ttttcctggg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaggc
     5821 ggggcaatcg cggcagttaa tctgcgtacc aattattggc ggaccgtccg gtaaactcga
     5881 aagccgctat cacgtaaaca cattaaccta cagcgcggca gcgaagctga aaggggaatc
     5941 gcatctcgcg gattttccgg ctctactgga taacccatta attcgtaatg ggatcatgca
     6001 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
     6061 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
     6121 gagtgacgac ctgaatgccc gccaggttgc tggcgatatt tgctcgcgct tttttgatat
     6181 tcacggcgaa atggttgaaa cgaatatgag cgaaaaaaca ctctctatcg aaatgaataa
     6241 attaaagcag gcacgatatt ccattggcat tgccatgagt gaagaaaaat acagcggaat
     6301 tattggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca gcacagctga
     6361 actattactg aaataaaaat cttcccgatg tttatcgtaa taaaaattac ggcgggtccc
     6421 tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg ataaaatcat
     6481 tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat tattagcaca
     6541 aggcgcaaac gtacagatgg tagatattca cggtggcgat ggacaatatg aaggtcataa
     6601 aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc atacggtagc
     6661 ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg gggtcaattt
     6721 cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca acgaagctgc
     6781 attcgaaaaa atggtcaata tcaaccagaa aggcgttttt ctgatgtcgc aggcggtggc
     6841 gcgacagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag aaagtgggct
     6901 ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca atagcttcac
     6961 gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta tcgcgccggg
     7021 gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg cgtggacgcg
     7081 caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc ctattgggcg
     7141 cgccggaaga ttagcagaaa tcgctgattt tgtttgttat ctgctgtctg aacgcgccag
     7201 ctatatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggct aaggaggctt
     7261 tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc tggaatcggt
     7321 tcagatggtt tacggcaacg ccaatgttga agccgttgaa tttgtgcccg gtgaaaacgc
     7381 cggagacatc gtagcaaagc tggaaaaatt agtaagcatt cacaatcaaa atgagtggct
     7441 gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc ttgcgatgcg
     7501 caatcctcgt ctgcgggtaa tcagcggact ttctctgccg ctggcgctcg aactggttga
     7561 taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa tcgcaaagca
     7621 aacctgcgtt gtctggaaac aactggcaac gacagaggag gatttctgat gaacattacg
     7681 ctcgcccgca ttgatgaccg cctgatccac ggtcaggtca ctaccgtctg gtcgaaagtg
     7741 gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga agttcgccgg
     7801 acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa tattgaaaaa
     7861 gccgtcgcgg tttatcacaa cccgcaatat caggacgaaa ccgtttttta tcttttcact
     7921 cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac cctgaatatt
     7981 ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc attagatgat
     8041 gatgatatta acgcatttca tgagttaaat aatctaggcg tcatactgga tttacgcgtc
     8101 gtagcgtcag atccctcaat caatattatc tacaaaataa acgaacagtt aatcgcaaat
     8161 taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca cttaaaaggt
     8221 gacaaatcat ggaaataagt accctacaaa tcattgctat atttcttttt tcctgtattg
     8281 ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt gcctgtacgg
     8341 tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt acgctggaat
     8401 tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct gcactcgcca
     8461 gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc ggaatcgcca
     8521 tcgcgttgcc tgtggctgcg gcaggccagg tgctgacggt gtttgcccgt accatcaccg
     8581 tagtgttcca gcacgcggcg gataaagcag cggaagaagc acggtttcgc actctcgata
     8641 ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt cgccattccg gcactgattg
     8701 tctcgctgtt cgtcagcgcc gatatggtga gcaatatgtt aaacgccatt cccgaatttg
     8761 tgacccgagg tctgcaaatt gctggcggtt ttatcgtggt agtcggttac gccatggtgc
     8821 tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc ctcgcaggtg
     8881 gctacctcga tctcagtctg ctggcgttcg gtggcgtcgg cgtgatcatg gccctggtct
     8941 acatccagct aaatccacag tggcgtaaag ctgaacctca actcctggcc cccgcttcca
     9001 ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa aattacacgc
     9061 ggcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt taactttgaa
     9121 cgtattcacg ggctgggttt ttgctacgac atgatccccg ccatcaagcg actttaccca
     9181 gtaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttctttaa taccacgcca
     9241 gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg ggccaacggc
     9301 gcggaaattg atgacggcac cattaacggc atcaaagtcg gtctgatggg accattggca
     9361 ggggttggcg atccactggt ctggggaacg ctgcgcccga ttaccgccgc gctcggcgca
     9421 tcgctggcgc tgtctggcaa cattctcggc ccgctgctgt tcttctttat tttcaacgcg
     9481 gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg ggtgaatatc
     9541 gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc gattctcgga
     9601 ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc gttggtggtt
     9661 tcacaaacgc atgccgccga tggctccacc gtcaccatga ccgtgcagaa cattctcgat
     9721 caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg tctgctcaac
     9781 aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat tatcggcaat
     9841 gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc atcgttcaac
     9901 atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga tttacgcctg
     9961 gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt ggtcactgac
    10021 agcctgtgcc tctcttcctg gaaagaggcc aatctgggtg aaaaccataa aaaagtaccc
    10081 gacgatgtgg cgaccaaccc aatcatcatc ggccacgagt tttgcggcga tattctggcc
    10141 gtgggtaaaa agtggcagca caaattccag ccgggtcagc gttatgtgat tcaggccaac
    10201 ctgcaactcc ccgaccgccc ggactgcccc ggctactcct tcccgtgggt aggcggcgag
    10261 gccacgcatg tggttattcc caacgaggtc atggaacaag attgcctgct ggcatacgac
    10321 ggcgaaacct attttgaagg ctcgctggtt gaaccgcttt cctgcgtgat tggcgcgttc
    10381 aacgccaact atcatcttca ggaaggtagt tataaccaca cgatggggat tcgcccgcaa
    10441 gggcgcatgc tgatcctcgg cggcaccgga ccaatgggac tgttggcgat tgattatgcg
    10501 ctacatggac ccgttaaccc gtcgctgctc gtcattaccg ataccgacaa cgataaattg
    10561 agttatgcgc gcaagcacta tccatcagaa ccgcaaacac tgattcatta tctcaatgcc
    10621 gccgatgcag catttgatac gctaatggcg ctgagtggcg gtcacggctt cgatgatatt
    10681 ttcgtctttg tgcctaatga aggactggtg actctcgcct cttccttgct ggcgacagat
    10741 ggttgcctga atttcttcgc cggaccgcag gataaacatt tcagcgcgcc aattaatttc
    10801 tacgatgtgc attatgcatt tacccactac gtgggcacgt caggcggcaa taccgacgac
    10861 atgcgcgcag cggtcaaatt gattgaagag aaaaaagtgc aggccgcaaa agtggtaaca
    10921 catattcttg ggctgaatgc cgcgggcgaa accacgcttg aattgcctgc cgtcggcggc
    10981 ggcaaaaagc tggtgtatac cgggaaatac ctgccgctga cgtcactcac gcagattcag
    11041 gatcaagcac tggcggcgat tctggcgcgt catcagggga tctggtccgg tgaggcggag
    11101 caatatctgc tcactcatgc agaggcaatt tcccatgatt aatcgcgcta cttaactctg
    11161 catgtcgctg gctggtcacc gcggcaactt cgcgacctga tgtgcggctg attgctgaag
    11221 cggcccaatg tgcacgagaa ggataaaaaa acatcagcgc cttaaggggc gctcaaattg
    11281 gtggcaaagt acacattgtt tatgccggat gcggaataaa gccttattcg gcctacataa
    11341 tctcgaaaat tcaatatatt gcagttatct ggtaggcctg gtaagcgtag cgcatcaggc
    11401 aatgctgtgt ttgtcatcag tctcagcgcc cggtgcaata cgtgacatcg tgagccatgt
    11461 cagtaagtgg ttaaattcgc agtggtagca tgccttccgt gatttcttac taaccggaga
    11521 agtaatggaa ctgcttttat tgagtaactc gacgctgccg ggtaaagcct ggctggaaca
    11581 tgcactgccg ctgattgctg aacagttgca gggtcgccgc tcagcggtgt ttatcccttt
    11641 cgctggcgta acgcagacct gggatgatta cacagcgaaa acggctgcgg ttctcgctcc
    11701 gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat cccgttgccg cgattgaaaa
    11761 tgcggaaatt gtgattgtcg gcggcggtaa tactttccag ttgctgaaac agtgccgcga
    11821 gcgcgggctg ctggctccaa ttactgacgt ggttaaacgt ggcgcgctgt atattggctg
    11881 gagcgcaggc gctaaccttg cttgcccaac cattcgtacc accaacgata tgccgattgt
    11941 cgatccgcaa ggtttcgatg cgctaaatct gttcccgttg caaatcaacc cgcacttcac
    12001 caacgcgctg ccggaaggcc ataaaggtga aacccgtgag cagcgtattc gcgaactgct
    12061 ggtcgtcgcg ccagaactga cgattattgg tctaccggaa ggtaactgga tcacagtgag
    12121 taaaggtcac gcaacgctgg gtggcccgaa taccacctat gtgtttaagg ccggtgaaga
    12181 agcggttccg ctggaagctg gtcaccgttt ttaagtgaat tgccggatgc atgatccggc
    12241 atcttgttca tagtgcctga tgcgacgctt gacgcgtctt atcaggccta caggattcac
    12301 aatattaata ctcgtcccgt ccctcatctt catcaggctg ttccagcaca ctgtaagcca
    12361 ccgaacaaaa cagcgagttc agacgctgca tatctcccag taagcctaaa tgtagcgaac
    12421 tggtttcaat gctttgcacg ttttgctgat gcaggcgatc gacgtgggcg tgggaatagc
    12481 ggcgattaag aatgcgaaag cgatgcttgc tgcggcgcaa acgacgagcg ctggtgacat
    12541 cgccagagaa gaacaccgac attgccagct ttaaattact gagcaattgc tcatagagcg
    12601 catccagttc cttcaagcca tcaagcgaaa atgctcgcct tgcagccagc gatttgtcgg
    12661 caatttcact actcatgcgc tcgacgatat cggaggcctg ttcaaggttg agcgacattt
    12721 cgatgatctc cgcccagcgg cgcgactctt cttctgccag ttcctctttt ggcatccgcg
    12781 ccagatacag cttaatggcg gtatagagaa cgttgatatc atctgccagc ttacgcagct
    12841 ctttctcctg ccttggctcg ccgtgcatca ctttattcag cccttccatc atctgttcca
    12901 tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc cagagcaagc gtgggcgtat
    12961 ccagcgcgct gacatccaga tgtttaggcc gtagctgggt atccagttcc ggttcatcgc
    13021 gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg gccaggcaac
    13081 gtacaaggtt gtagaagacg tggaaataga tcaccagttc cgcttttggc aatgacaact
    13141 tccccattgt ctctgccagc aaatggacaa acggcaggat aatcaggcta cccaccagct
    13201 taaacagcag actacccagc gcgacacggc gggctgcggc attggcggca ctgttgttga
    13261 gcatcgccag caggccggaa ccgaggttag caccaatcac cagacagagc gccacgggga
    13321 aggagataat gcctgcggcg gtcagagtcg cggtcagcag tacagcagca aggctggagt
    13381 agctgataat ggcgaacatc gcgccaatca gcgcatccag cagaatatcg ccggtcagcg
    13441 aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc tgcacaatca
    13501 actccagcgc cagcaaaatc agcccaagac caataccgac gcggcccagt tgcccggcgc
    13561 gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa aataagtaac ggtgacagcc
    13621 aggataagtc gaaggtgaga atacgcgcca ttagcgccgt cccgacatct gcccccagca
    13681 caatgaccag agccggtgcg agggctacca gatcctgagc gacgaacgag gtcaccagca
    13741 tggtggtggc attactgctc tgtaccagtg cggtaacgcc gatccccgcg caaaaggcga
    13801 gcggcttttt ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg aagacgcgca
    13861 ttacgccggt tcgaacaata tgagtccccc agaccagcag ggcgacggca gaaagcaggt
    13921 taagcagcgt taacacgagt tcaggttctc cttatcgtta tatgttcctg aagcaattga
    13981 atgaccaaag gaccggaaac acgctgactg gcatctttac tgtgcctttt gacattcgtt
    14041 tttccggcta agattattcc agcctgtttc cagtataagt gtttcatatt aataaagaga
    14101 cagggcactc attgagcgcc ctgtttgttg taaggaaaag tgacagattt gtgaatcagt
    14161 ccgcgtcata ccccagattc ggtgccagcc agcgctcaac atcggagacg ctcatacctt
    14221 tacggcgggc atagtcttca acctgatcgc gctgaatttg cgccaccgcg tagtacttgc
    14281 tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc gggccacatg gcgaaagatt
    14341 ctgtgagttt catgccggta tgtttttcca cttccagcag ctcccagatg gtggcttttt
    14401 ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg gatgccctgg tagttttcgc
    14461 ggatcagttc ttcgttgctg aggttctcgt tcggcgcata gccccagtag actttacgca
    14521 cacgctcatg gagatactcc gcaaaggctt cggctaaacg gtcggcaagc gctttcacca
    14581 tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg tcctcttcca
    14641 gcccgccagt cacggcaaat gcgccgatgt aatctgcttt accagaaagc ttcggcgcaa
    14701 cgaagtcagc gagacagtag ttagcgaatc ccgttttttc ggtctgttga cgcagatggt
    14761 ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta gatttcaatg tcatcgccca
    14821 cacggtttgc cgggaacagg cccaccacgc cacgcggatt cagcgttttc tcggcgctta
    14881 atttatccag catgtcgttg gcgtctttaa acagccgctg cgcctcaacg cccaccactt
    14941 catcttccag aatgcgcgga tacttcccgg ccagcgacca ggtcataaag aacggtgtcc
    15001 agtcgatgta attacgcaga gtttcgatgc tggcttcaac ttcctgcacg ccgagacggt
    15061 gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa gtcgttatcg cgcgccgctt
    15121 ccagcgtgac cggtggtgtg cgtggtttct tgcgcccgtg ctgaatacgt acggtttcgt
    15181 actccttgcg ggtacgagcg acaaaatcat cgcgctgagt atcggaaagc agcgccgcca
    15241 ccacaccaac ggtgcgcgag gcattctgca catacaccgt cgggccgctg tagttctgct
    15301 cgattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc accgatcagt aacggaatgg
    15361 tgaagccctg acgctccatc tctttcgcca cgttaaccat ctcgtccagc gacggcgtga
    15421 taagccccga aagtccaatc agatcagcat tcacttcttt agcggtacgg agaatttttt
    15481 ccgcaggcac cataacgccg agatcgacaa tttcgtagtt gttacattgc agcaccacac
    15541 caacgatatt tttaccgatg tcgtggacgt cgcccttcac ggtggcgatc accatcttgc
    15601 cgttggtttt gccctgctct ttgctggctt caataaacgg ttcgaggtag gccactgcct
    15661 gcttcatcac acgcgccgat ttgaccacct gtggcaggaa cattttccct tcgccgaaca
    15721 gatcgccgac cacgttcatg ccgtccatta gcgggccttc aatcacttca atcgggcgcg
    15781 tagcctgctg gcgggcttct tcggtatcct gctcgataaa ctcagtaatg cctttgacca
    15841 gcgagtattc cagacgttta ttcacttccc acgagcgcca ctccgcctgc tgggcgttag
    15901 cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag ctccagtaaa cgctcagtgc
    15961 catcatcgcg acgattaaga atcacatctt ccaccgcgtc gcgcagttca gcgggcaggt
    16021 cgtcgtaaat cgccagttgc ccggcgttga cgatccccat atccatgcca ttgcgaatag
    16081 cgtagtagag gaacaccgcg tgaatggctt cgcgcaccgg atcgttgcca cggaatgaga
    16141 aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg cagttcgcgt ttgatgtctt
    16201 cgcaggcgcc gataaagtcc tgcgcatagt tgttgtgctc ttcaatgccg gtggcgaccg
    16261 cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa gccaacctct tcggtgagga
    16321 ttttgtacgc ccgacggcaa atctcaattt tccgtgcgcg ggtatcggcc tgcccctgtt
    16381 cgtcaaaggc cattaccacc accgctgcac cgtaacgacg caacagtttc gcatgatgga
    16441 taaaggcgtc gacgccctct ttcattgaga tagagttaac aatgcctttg ccctgaatac
    16501 acttcagccc cttttcgatg acctcccatt ttgaggagtc gatcataatc ggcacgcgag
    16561 cgatatccgg ttcaccggca atcagattga gaaaacgcac catcgccgct tcggcatcga
    16621 gcatcccttc atccatgttg atatcgataa tctgcgcgcc gttttccacc tgctgacgcg
    16681 ctacatccag cgcctcgctg tatttctctt ctttgatcag gcgcttgaac ttagcggaac
    16741 cggtgacgtt ggtgcgttca cccacgttca caaacaggct atcgtcgcca atgttcagcg
    16801 gctccaggcc ggacaaacgg caggctacgg gaatttcagg cagtttgcgc ggcgctaatc
    16861 cttctactgc acgactcatc gctgcaatat gttgtggcgt ggtgccacag cagccgccga
    16921 cgatattgag aaaacccgct tgcgcccatt cacgtatctg ttttgccatc gtgtcggcgt
    16981 cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt cgggtgcgcg gtgacgtagc
    17041 attccgcaat ccgtgatagt tcctgcacgt actggcgcag ttcatctggc cccagcgcgc
    17101 agttgaggcc aaaagtcagg gcttcggcat ggcgcaggga gttgtaaaat gcttcggtgg
    17161 tctgtccgga gagcgtgcgc ccggaggcgt cggtgatggt gccggagatc ataatcggca
    17221 gctcaacgcc taacgcttcg aactccgttt tcaccgcaaa gaccgccgct ttggcgttaa
    17281 gggtgtcgaa aacggtttca atcagaatca gatccgcacc gccttccact aatgctttgg
    17341 tggattcgcg ataggccgcc accagctggt caaaagtgat gttgcggaat gccggatcgt
    17401 tcacgtccgg agaaatggac gccgtgcggt tggttgggcc gagaacgccg gcaacgtagc
    17461 gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca ggcgcgcgcc agtttcgccg
    17521 ccgcaaagtt gatttccgcc gacagggatt ccatctggta atccgccatc gcaatggtcg
    17581 tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc ttcaaagtag gcgttgtgga
    17641 tagcggcgat cacttccggt ttgcttagca ccagcagatc gttattgcct ttgaggtcgc
    17701 atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga tagctctgga
    17761 tcatggtgcc cataccgccg tccagcacca gaatacgttc atttaactgc gcacgcagtt
    17821 gttccacttt gctgctcaca cttgctcccg acacgctcaa cccagattta ataaaaattc
    17881 aacaaaccat actggcataa acgcatctgt ggtaaaagcg accaccacgc aacatgagat
    17941 ttgttcaaca ttaactcatc ggatcagttc agcaactatt gcattagcta acaataaaaa
    18001 tgaaaatgat ttccacgata cagaaaaagg agactgtcat ggtcgcaccc attcccgcga
    18061 aacgcggcag aaaacccgcc gttgccaccg caccagcgac tggacaggtt cagtctttaa
    18121 cgcgtggcct gaaattactg gagtggattg ccgaatccaa tggcagtgtg gcactcacgg
    18181 agctggcgca acaagccggg ttacccaatt ccacgaccca ccgcctgcta accacgatgc
    18241 aacagcaggg tttcgtgcgt caggttggcg aactgggaca ctgggcaatc ggcgcacatg
    18301 cctttatggt cggcagcagc tttctccaga gccgtaattt gttagcaatt gttcacccta
    18361 tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa tatggcggtg cttgatcaaa
    18421 gcgatcacga agcgattatt atcgaccagg tacagtgtac gcatctgatg cgaatgtccg
    18481 cgcctatcgg cggtaaattg ccgatgcacg cttccggtgc gggtaaagcc tttttagccc
    18541 aactgagcga agaacaggtg acgaagctgc tgcaccgcaa agggttacat gcctataccc
    18601 acgcaacgct ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg cgcaaacggg
    18661 gttattcatt tgacgatgag gaacatgcgc tggggctacg ttgccttgca gcgtgtattt
    18721 tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc cggaccgatt tcacgtatta
    18781 ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa agcggcgaag gaagtgacgc
    18841 tggcgtacgg tggaatgcgc tgactttttc tggcgggcag aggcaatatt ctgcccgtca
    18901 tacctgagtg gtaatagaat aagggtgtct gttaatcgca ttgacgtcaa aataacttat
    18961 tgtcatacac ttcatacact tttattaact cagcattatt tttaaacatc aaaccactta
    19021 attattaaca aaatcgcaag atctcattcc accgaaactt ttacggctgc attatgagat
    19081 tgagcgattt a
//
LOCUS       DAZKIF010000096        18587 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZKIF010000096 DAZKIF010000000
VERSION     DAZKIF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.096"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            95..274
                     /locus_tag="ACHOTW_003306"
     CDS             95..274
                     /locus_tag="ACHOTW_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845356.1"
                     /translation="MQQLVPPPGVKGIDKMVHYEVVQYLMDCCGITYNQAVQALRSND
                     WDLWQAEVAIRSNKM"
     gene            285..440
                     /gene="ydaF"
                     /locus_tag="ACHOTW_003307"
     CDS             285..440
                     /gene="ydaF"
                     /locus_tag="ACHOTW_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaF family protein"
                     /protein_id="HFP9845357.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LEKHTVS"
     gene            complement(437..925)
                     /gene="sieB"
                     /locus_tag="ACHOTW_003308"
     CDS             complement(437..925)
                     /gene="sieB"
                     /locus_tag="ACHOTW_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion B family protein"
                     /protein_id="HFP9845358.1"
                     /translation="MLDVFTPLLKLFANEPLERLMYTIIIFGLTLWLIPKEFTVAFNA
                     YTEIPWLFQIIVFAFSFVVAISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFL
                     KTGNLIITSPCRNPVMKKLERKGIIQHQSDSANCSYYLVTEKYSHFMKLFWNSRSRRF
                     NR"
     gene            1367..1588
                     /gene="kilR"
                     /locus_tag="ACHOTW_003309"
     CDS             1367..1588
                     /gene="kilR"
                     /locus_tag="ACHOTW_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFP9845359.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            1588..1758
                     /gene="ydaE"
                     /locus_tag="ACHOTW_003310"
     CDS             1588..1758
                     /gene="ydaE"
                     /locus_tag="ACHOTW_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFP9845360.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            1833..2108
                     /locus_tag="ACHOTW_003311"
     CDS             1833..2108
                     /locus_tag="ACHOTW_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845361.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPTEKKAEFLATLIAMKLKGEI"
     gene            2210..4810
                     /gene="recE"
                     /locus_tag="ACHOTW_003312"
     CDS             2210..4810
                     /gene="recE"
                     /locus_tag="ACHOTW_003312"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFP9845362.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETA
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            4803..5612
                     /gene="recT"
                     /locus_tag="ACHOTW_003313"
     CDS             4803..5612
                     /gene="recT"
                     /locus_tag="ACHOTW_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFP9845363.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            5668..5817
                     /gene="ralR"
                     /locus_tag="ACHOTW_003314"
     CDS             5668..5817
                     /gene="ralR"
                     /locus_tag="ACHOTW_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFP9845364.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            5855..6043
                     /locus_tag="ACHOTW_003315"
     CDS             5855..6043
                     /locus_tag="ACHOTW_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP9845365.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            6143..6358
                     /gene="xisR"
                     /locus_tag="ACHOTW_003316"
     CDS             6143..6358
                     /gene="xisR"
                     /locus_tag="ACHOTW_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFP9845366.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            6360..7595
                     /gene="intR"
                     /locus_tag="ACHOTW_003317"
     CDS             6360..7595
                     /gene="intR"
                     /locus_tag="ACHOTW_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9845367.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEVTNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGVVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            7647..8582
                     /gene="ttcA"
                     /locus_tag="ACHOTW_003318"
     CDS             7647..8582
                     /gene="ttcA"
                     /locus_tag="ACHOTW_003318"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP9845368.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(8711..10084)
                     /gene="dbpA"
                     /locus_tag="ACHOTW_003319"
     CDS             complement(8711..10084)
                     /gene="dbpA"
                     /locus_tag="ACHOTW_003319"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP9845369.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(10117..10287)
                     /gene="ynaL"
                     /locus_tag="ACHOTW_003320"
     CDS             complement(10117..10287)
                     /gene="ynaL"
                     /locus_tag="ACHOTW_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP9845370.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(10562..11545)
                     /gene="zntB"
                     /locus_tag="ACHOTW_003321"
     CDS             complement(10562..11545)
                     /gene="zntB"
                     /locus_tag="ACHOTW_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP9845371.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(11559..11606)
                     /gene="ydaN"
                     /locus_tag="ACHOTW_003322"
     CDS             complement(11559..11606)
                     /gene="ydaN"
                     /locus_tag="ACHOTW_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP9845372.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            11800..13032
                     /gene="dgcM"
                     /locus_tag="ACHOTW_003323"
     CDS             11800..13032
                     /gene="dgcM"
                     /locus_tag="ACHOTW_003323"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP9845373.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(13053..13616)
                     /gene="smrA"
                     /locus_tag="ACHOTW_003324"
     CDS             complement(13053..13616)
                     /gene="smrA"
                     /locus_tag="ACHOTW_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP9845374.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(13946..14854)
                     /gene="abgR"
                     /locus_tag="ACHOTW_003325"
     CDS             complement(13946..14854)
                     /gene="abgR"
                     /locus_tag="ACHOTW_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9845375.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            15030..16340
                     /gene="abgA"
                     /locus_tag="ACHOTW_003326"
     CDS             15030..16340
                     /gene="abgA"
                     /locus_tag="ACHOTW_003326"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFP9845376.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            16340..17785
                     /gene="abgB"
                     /locus_tag="ACHOTW_003327"
     CDS             16340..17785
                     /gene="abgB"
                     /locus_tag="ACHOTW_003327"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFP9845377.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            17822..18587
                     /locus_tag="ACHOTW_003328"
                     /pseudo
     CDS             17822..18587
                     /locus_tag="ACHOTW_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
BASE COUNT         5176 a         4426 c         4537 g         4448 t
ORIGIN      
        1 acgccgggca atacttcgag ttaccaggca gtggtcaggg gttaagtagc cagcccgagg
       61 cgtatgaaca tgacggcggg attcaaattt tgcagtgcag cagttagttc cgccacccgg
      121 cgttaagggg atagataaga tggtgcatta cgaagtagtt cagtatttga tggattgttg
      181 cggtatcact tacaaccagg ctgtgcaggc tttacgcagc aacgactggg atctctggca
      241 ggcagaagtc gctatacgta gcaacaagat gtgagattcg caaaatgcaa aaaatcgacc
      301 tcggcaacaa cgaatccctg gtgtgcggcg tgttccccaa ccaggatgga acgttcactg
      361 ccatgacgta taccaaaagc aaaacattta aaaccgaaac tggtgcgcgc cgatggttgg
      421 agaagcacac agtaagctaa cgattaaaac gtctactcct gctgttccag aataacttca
      481 taaaatggga gtatttttcg gtgacgagat aataagaaca gtttgcgcta tcactctgat
      541 gttgaatgat gcccttccgt tctaattttt tcataaccgg gttacggcaa ggagaagtga
      601 taataagatt tcctgtttta aggaaatctt taaatacagc gatttctttc tcagataaac
      661 gaagcaatac tcgttgctct ggtagtaatg aataatgctt ttgaatatgt gctcgcaatc
      721 ttgagaagga aatggcgacc acgaaagaaa aggcaaaaac gataatctga aagagccaag
      781 gtatttcagt ataagcattg aatgcgacag taaactcttt cggtatcagc cagagagtga
      841 gaccaaaaat gataatcgta tacataagtc tttcgagtgg ctcgttagca aaaagtttca
      901 acaatggagt aaatacatcc aacatatcaa taactctcaa ctgtaagggt attgaaatgt
      961 taacacaagc tctcgctgta ggggtatagc cgagaccacc gaagcccgga ggtggtgaaa
     1021 taaaaccggg cacaacacga aggcgcattt ccgatatcca taaagagtcg gtcttgtctg
     1081 ttaaatttaa atggtgggag tgcgcctccg gttgtaaata acgacattgc tgtgtgtagt
     1141 cctggcggca tcagtttttt tcttgaagtt cggctgatgt ccgccctttt taaagtgaat
     1201 tttgtgatgc gatgaatgcg gctaagcgca cgtggcacag ttaaaagtca tgttagtcct
     1261 tattggtttg ggtgggaaag ccgactgtaa ttgttaactg gttgcagtca cctggaggca
     1321 ccaggcaccg catcaacaaa gttcatttgt aaaaatggag ataattatga ttgcacatca
     1381 cttcggaact gatgaaatac cacgtcagtg tgtgactcct ggcgattatg ttcttcatga
     1441 aggccggaca tatattgcct cggcaaacaa tattaaaaag cgaaaactat atattcgtaa
     1501 cctgaccaca aaaacatgca ttactgaccg catgattaaa gtcttcctcg gtcgtgatgg
     1561 tttacctgta aaggcggagt catggtgatg actaagaaaa taaaatgtgc ttaccacctt
     1621 tgcaaaaaag acgttgaaga aagcaaagct attgaaagaa tgcttcactt catgcacggg
     1681 attttatcaa aagacgaacc gagaaaatat tgcagtgaag cttgtgccga aaaagaccag
     1741 atggcacatg aactttaatt aattgactat tcgaaactga atttatgcca gaaatggcag
     1801 gtattcgctc aaccttaatt aaggagaaaa acatgattac caattatgaa gccactgttg
     1861 taactaccga tgacattgtt cacgaggtga atctggaagg aaagcgcatt ggctacgtaa
     1921 ttaaaacaga aaataaagaa accccattca ctgtggttga tatcgatggt ccatcaggca
     1981 acgtaaaaac acttgatgaa ggtgtcaaaa aaatgtgcct ggtgcatatc ggaaagaatc
     2041 tgcccacaga aaaaaaagcc gaatttctgg caactctaat tgcaatgaaa ttaaaaggtg
     2101 aaatctgaaa gaaatagcct gcgtatggcg caggctatga acagtgtgta tccggcaaga
     2161 tcattcactg aacaaaacga attttaatct gagttgaggt taaaaaacaa tgagcacaaa
     2221 accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg tcgtcctgtg
     2281 ggcaagcaac gattttgaat cgacctgtgc cactctggac tacctgatcg ttaagtcagg
     2341 taaaaaactg agcagctatt ttaaagctgt tgccacgaat tttcctgtcg ttaatgacct
     2401 gcccgctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca gcaaagactc
     2461 catgacatgg gaactaaaac cgggagcagc accagacaac gctcactatc aaggcaatac
     2521 caacgtcaac ggcgaagaca tgactgagat tgaggagaat atgctactcc caatttctgg
     2581 ccaggaactg cccattcgtt ggcttgctca acacggcagc gaaaaaccgg taacgcacgt
     2641 ttcacgcgac ggactccagg cattacacat tgctcgggct gaagaactac cggctgttac
     2701 tgccctggct gtttcccaca aaaccagcct gctcgacccg ctggaaattc gcgaactcca
     2761 caaactggtt cgtgacactg acaaagtttt ccctaatcct ggtaattcaa acctgggact
     2821 gataactgct tttttcgaag catacctgaa cgctgactac accgatcgag gactgctgac
     2881 aaaagagtgg atgaagggta atcgtgtttc acacatcact cgcacggctt ccggtgctaa
     2941 tgctggcggc ggaaacctca ccgatcgcgg cgaaggtttc gtacacgatc tgacgtcact
     3001 ggcgcgcgac gtagccactg gcgtactggc ccgttcaatg gatctggaca tctataacct
     3061 tcatccggca cacgctaaac gcattgagga aattatcgct gaaaataaac cgcccttttc
     3121 tgttttccgc gacaaattca tcaccatgcc tggcgggctg gattattccc gcgccatcgt
     3181 ggttgcgtcc gtaaaagaag caccaattgg gatcgaggtc atccccgcgc acgtcactga
     3241 atatctgaac aaagtactga ctgaaaccga tcatgccaac cctgatccgg aaatcgtgga
     3301 tattgcctgc ggtcgctcct ctgccccgat gccgcagcga gtaacagaag aaggaaaaca
     3361 ggatgatgaa gaaaaaccgc aaccatctgg aacaacggca gttgaacagg gagaggctga
     3421 aacaatggaa ccggacgcaa ctgaacatca tcaggacacg cagccgctgg atgctcagtc
     3481 acaggtaaat tctgttgatg cgaaatatca ggaactgcgg gcagaactcc atgaagcccg
     3541 gaaaaacatt ccatcaaaaa atcctgtcga tgccgataaa ttgcttgctg catcacgtgg
     3601 tgaatttgtt gacggaatta gcgacccgaa cgatccgaaa tgggtaaagg ggatccagac
     3661 tcgcgattgt gtgtaccaga accagccaga aacggaaaaa accagcccgg atatgaatca
     3721 acctgagcca gtagtgcaac aggaaccgga aatagcctgc aatgcctgcg gccagactgg
     3781 cggggataac tgccctgact gtggtgcggt gatgggcgac gcaacatacc aggaaacatt
     3841 cgatgaagag agtcaggttg aagctaagga aaatgatccg gaggaaatgg aaggcgctga
     3901 acatccgcac aatgagaatg ctggcagcga tccgcatcgc gattgcagtg atgaaactgg
     3961 cgaagtcgca gatcccgtaa tcgtagaaga catagagcca ggtatttatt acggaatttc
     4021 gaatgagaat taccacgcgg gtcccggtgt cagtaagtct cagctcgatg acattgctga
     4081 tactccggca ctgtatttgt ggcgtaaaaa tgcccccgtg gacaccacaa agacaaaaac
     4141 gctcgattta ggaaccgctt tccactgccg ggtacttgag ccggaagaat tcagtaaccg
     4201 ctttatcgta gcacctgaat ttaaccgccg gacaaactcc ggaaaagaag aagagaaagc
     4261 gtttctgagg gaatgcgcaa gcacaggaaa aacggttatc actgccgaag aaggccggaa
     4321 aattgaactc atgtatcaga gcgttatggc tttgccgctg gggcaatggc ttgttgaaag
     4381 cgccggacac gctgaatcat caatttactg ggaagatcct gaaacagcaa ttttgtgtcg
     4441 gtgccgtccg gacaaaatta tccctgaatt tcactggatc atggacgtga aaactacggc
     4501 ggatattcaa cgattcaaaa ccgcttatta cgactaccgc tatcacgttc aggatgcatt
     4561 ctacagtgac ggttatgaag cacagtttgg agtgcagcca actttcgttt ttctggttgc
     4621 cagcacaact attgaatgcg gacgttatcc ggttgaaatt ttcatgatgg gcgaagaagc
     4681 aaaactggca ggtcagctgg aatatcaccg caatctgcga accctggctg actgcctcaa
     4741 taccgatgaa tggccagcta ttaagacgtt atcactgccc cgctgggcta aggaatatgc
     4801 aaatgactaa gcaaccacca atcgcaaaag ccgatctgca aaaaactcag ggaaaccgtg
     4861 caccagcagc aattaaaaat aacgacgtga ttagttttat taaccagcca tcaatgaaag
     4921 agcaactggc agcagctctt ccacgccata tgacggctga acgtatgatc cgtatcgcca
     4981 ccacagaaat tcgtaaagtt ccggcgttag gaaactgtga cactatgagt tttgtcagtg
     5041 caatcgtaca gtgttcacag ctcggacttg agcccggtag cgccctcggt catgcatatt
     5101 tactgccttt tggtaataaa aacgaaaaga gcggtaaaaa aaacgttcag ctaatcattg
     5161 gctatcgagg catgattgat ctggctcgcc gttcaggtca aatcgccagc ttgtcagccc
     5221 gtgttgtccg tgaaggtgac gagtttaatt tcgaatttgg ccttgatgaa aagttaatac
     5281 accgcccagg agaaaacgaa gatgcccctg ttacccacgt ctatgctgtc gcaagactga
     5341 aagacggagg tactcagttt gaagttatga cgcgcaaaca gattgagctg gtgcgcagcc
     5401 agagtaaagc tggtaataac gggccgtggg taactcactg ggaagaaatg gcaaagaaaa
     5461 cggctattcg tcgcctgttc aaatatctgc ccgtatcaat tgagatccag cgtgcagtat
     5521 caatggatga aaaggaacca ctgacaatcg atcctgcaga ttcctctgta ttaaccgggg
     5581 aatacagtgt aatcgataat tcagaggaat aattcagcct ggcggtgtaa tgcaccgcca
     5641 acttgaaata tttttatgag aaaaattatg agatatgaca atgttaaacc atgtccattt
     5701 tgtggttgtc catcagtaac ggtgaaagcc atttcaggat attaccgagc gaagtgtaac
     5761 ggatgcgaat cccgaaccgg ttatggtgga agtgaaaaag aagcactcga acgatgaaat
     5821 aaacgaacca ctggaaataa taatggaggt gttcatgtat aaaattaccg ccactattga
     5881 aaaggaaggt ggcactccta ctaactggac aagatattca aaatctaaac taacgaaatc
     5941 agaatgcgaa aaaatgctct caggtaaaaa agaagcaggc gtttccagag agcagaaagt
     6001 aaaactgata aattttaatt gcgagaaact tcagtcctcg tgaattgcat tgtattcaaa
     6061 ttaaaacttc atagctgatt attaataatc aacatcgggc gtcaatttaa gtctaacatt
     6121 ggcgcctgcc agaggtgatg cgatggcaca agtaatcttt aatgaagagt ggatggttga
     6181 atacggcctg atgcttcgca ctggtctggg ggccagacaa attgaagcat accgccagaa
     6241 ctgttgggtg gaaggcttcc acttcaaacg agtatctcct ttagggaagc cagacagtaa
     6301 gcgagggatt atctggtata actatccaaa gataaatcag tttatcaaag actcatgata
     6361 tgtctaaatt accaacaggt gtcgagattc gaggtaaata cattcgcatc tggttcatgt
     6421 ttcgaggaaa acgatgtcgg gaaacattga aaggctggga ggttactaac agtaacatta
     6481 aaaaagccgg gaatttaaga gcgttgatag ttcatgaaat caattccggt gagtttgagt
     6541 atttaagacg ttttccccag tccagcactg gggcaaaaat ggtgacaacg agggtcataa
     6601 aaacgttcgg ggagctttgt gatatctgga caaaaattaa agaaacagag ttaacaacaa
     6661 acacaatgaa gaaaacgaaa tcacaattaa aaacactcag gataataatt tgtgagagta
     6721 ctccgatatc gcatattcgt tatagcgata tcttaaacta ccggaatgaa ctgctgcatg
     6781 gagaaacgct ttacctggat aatccaagat ccaacaaaaa aggaagaacc gtgcgcacag
     6841 ttgataacta tatcgccctg ctctgttcgt tgttacgttt tgcgtatcag tcgggattta
     6901 tatcaaccaa accatttgaa ggagtaaaga aattacagag aaacagaata aagcctgacc
     6961 cgttatctaa aacagaattc aatgcattaa tggaaagtga aaaaggacag agccagaact
     7021 tgtggaaatt tgccgtatac tccgggcttc gtcacgggga actggctgct ctggcgtggg
     7081 aggatgtgga tttcgagaag ggagttgtga atgtcagaag aaacctgacg atactggata
     7141 tgttcggtcc cccaaaaaca aatgcgggga tccggacggt aacactactg cagcctgctc
     7201 ttgaagcact gaaggagcaa tacaaactga ccgggcatca tcgcaaaagc gaaatcacct
     7261 tttatcatcg ggagtacggc agaaccgaaa agcaaaaact gcattttgtt ttcatgccca
     7321 gggtgtgtaa cggaaaacaa aaaccttatt actcggtaag cagtttgggg gcaaggtgga
     7381 atgcagcagt aaaacgtgct ggtattcgcc gccgtaatcc gtaccatacg cggcatactt
     7441 ttgcctgctg gctgttgacg gcaggagcga acccggcatt tatagccagc caaatggggc
     7501 atgaaactgc gcagatggtg tatgaaattt acggtatgtg gattgatgac atgaacgacg
     7561 aacaggtagc catgttgaat gcgcggttat catagttgca aagtttgccc ccaatttgcc
     7621 ccatttagta ccagagaact gaaataatgc aagaaaatca acaaattaca aagaaagaac
     7681 aatacaacct gaacaaatta caaaaacgcc tgcgtcgtaa cgtgggcgaa gccattgctg
     7741 acttcaatat gattgaagaa ggcgatcgca tcatggtttg cctctccggg ggtaaagaca
     7801 gctataccat gctggagatt ctgcgcaatt tgcagcaaag cgcgccaatc aatttttcgc
     7861 tggtggctgt taacctcgat caaaagcaac cgggcttccc ggaacacgtt ctgcccgagt
     7921 atcttgaaaa gctgggcgtt gagtacaaga ttgttgaaga gaacacttac ggtatcgtga
     7981 aagagaagat tccagagggc aaaaccactt gctcactgtg ttctcggcta cgtcgcggta
     8041 tcctttatcg caccgcaacg gaactggggg cgacgaagat cgcgttgggc caccatcgtg
     8101 acgatatcct gcaaacgttg ttcttaaata tgttctacgg cggcaagatg aaaggtatgc
     8161 ctccgaaact gatgagcgat gatggcaaac atatcgttat tcgtccgctg gcctactgcc
     8221 gcgagaaaga tattcagcga tttgccgatg caaaagcgtt cccgattatt ccgtgcaacc
     8281 tgtgcggttc acagcctaac ctgcaacgtc aggtgattgc tgacatgttg cgtgactggg
     8341 ataaacgtta tccagggcgt atcgagacga tgttcagcgc gatgcagaat gtggtgccgt
     8401 cgcatctgtg cgataccaac ctgttcgatt tcaaaggcat cacccacggt tctgaagtgg
     8461 ttaacggggg tgatctggcg tttgatcgcg aagagatccc actacaaccg gcgggctggc
     8521 agccagaaga agatgaaaat cagttggatg agttacggct gaatgtggtt gaagtgaaat
     8581 aactcggata gcgcccgatg cgcaaacgta tcgggctact ctaatggagg ctgaataaga
     8641 cgcggtcagc gtcgcatccg acaatcccga cgaagatgtt tacttttgca tcaggcaatt
     8701 caacatttta ttattttaat aaccgcaccc ggcacgtttt tcctttaatc ttcccgccct
     8761 gtaactgttt ccatgcttta tgagcaacag cctgacggac cgcgacatag acatgcgccg
     8821 gatgcacggc gattttgcca atatctgcgc catcaagccc gatatctcct gtcagtgcac
     8881 ctaatacatc acccgggcgc attttggctt ttttcccgcc atcgatacac aacgttgcca
     8941 tttctgcttc cagcgtcaca atggaactat tagctggtgg cgtttgccag ttaagtttta
     9001 tctgcaacat gtcagaaatg atattggccc gctgtgcttc ttccggagca cagaaactga
     9061 tcgccagacc gctatttcct gcacgagctg tacgaccgat gcgatgtaca tgaacttcag
     9121 ggtcccacgc cagctcaaag ttcaccacca gctcaagcga tttaatatcc agaccacgcg
     9181 cagcaacatc agtcgcgacc agtacacggg cgctaccgtt agcaaaacgt accagggtct
     9241 gatcacgatc gcgttgctcc agatcgccgt gtaatgacaa tgcactttgc cctacttcat
     9301 tcagcgcgtc gcagacagcc tggcaatctt ttttggtatt gcaaaacacc acgcaagagg
     9361 atggctgatg caagcttaat aaccgttgca acagaggaat tttgcctttg ctggatgtct
     9421 cataaaattg ttgttcaatg ggtggcaaag catctgttga gtcaatttca atcgccaaag
     9481 gatcgcgttg cactcgtccg ctgattgcgg cgatggcttc cggccaggtt gccgaaaaca
     9541 gaagcgtctg tcgagatgca ggcgcaaaac ggatgacatc atcaatggca tcgctaaatc
     9601 ccatatccag catgcggtcg gcctcatcca tcaccaaagt attcagcgca tccagtgata
     9661 ccgtgccttt ttgcaggtga tccagcaaac ggcccggcgt tgccacgata atatgcggcg
     9721 catgttgcaa cgaatcacgc tgcataccga acggttgacc accgcacaac gtcaaaattt
     9781 tggtatttgg cagaaaacgc gccagccgac gcaattcacc tgccacctga tccgccagtt
     9841 cacgcgtagg acacagcact aaagcctggg tttgaaatag cgacgcatca atttgctgta
     9901 acaagccgag gccaaaagcc gccgttttgc cgctgccagt tttcgcctgc acgcgaacat
     9961 cttttccggc aaggatcgcc ggaagcgcgg cggcctgcac cggcgtcatg gttaaataac
    10021 ccaactcatt aaggttcgtg agttgggcgg gaggcaaaac attcagggta gaaaaagcgg
    10081 tcacaatcta ttctcgtggt catcgacgca aagtcatcag gcgcgtatcc tcgcagatct
    10141 acgctcacga tgcgacaatt taatcggttc ttcatcgggt ggtgggtcag gcatgggttg
    10201 cgggcgaggg atcggatcgg gcactggaac aggatcgcca ggaatcggtt cagggacagg
    10261 aatttgcaaa taaataagtg tcgtcatatt tccctccggt cattgggtgg actcttaaag
    10321 ggtagacgct gataaataac aggcaaaaaa aagccgactc atcaaagtcg gcgtcgtacg
    10381 aatcaattgt gctatgcagt aattcaaaaa aggaagtaag acaatatgga gcgcaacgcc
    10441 catcgcttga cgttgcattc acctgcaaga gagatattgc cctgaatggg tagagagttt
    10501 attgacttcg ctcaaacttt gcggcgtttt tgtatacaga cagccggaaa aattgctttt
    10561 gttacaacca tttactacga tgcaaccata aagcaacacc accaataaga acaactaaca
    10621 gaatacaaaa aattgaaaat ccgaattgcc acccgccgcc agggatccca ccaaggttga
    10681 cgccaaataa ccctgtcaga aaggtactgg gtaaaaagac cattgccatc aatgacattg
    10741 tataggtacg acgagctaaa ttttcctgca tcacctgagc gatttcatcc gccatcacac
    10801 cagtccgtgc tatacaggcg tcgatttcgt caaggccgcg cccaaggcga tcggcaatat
    10861 cctgcatccg acggcgttgg tcatcgctca tccacggcaa acgttcactg gcaagacgag
    10921 cataaacatc acgttgcggt gccatatagc gacgcatcac aattaattgt ttgcgcagca
    10981 gagccaggaa tccacgcggc ggaatttgct gatcaaggag attatcttca aggtcgataa
    11041 ttttatcgtg cagctgctcg ataaattcgc tggaatgatc ggtcaacgca tcgcacacat
    11101 ccaccagcca tcccccgcaa tcggtcggac ccgtgccctc ttccagatca ctcaccacat
    11161 cgtccagcgc cagcactttg cgttgtcggg tcgaaacaat taaccgcccg tccatatata
    11221 cacgcatggc gaccagttga tcggggcgtt catcggtgct gccgtttata cagcgcaatg
    11281 taatcagcgt gccttcaccg agacggctga ctcggggccg tgtgctctcg cccgccagcg
    11341 catcacgtac gttattggga agcagcggtg ttgtcgccag ccattgggcg ctatcatggt
    11401 gtacataatt aaggtggagc cagcagggat gcgcttcatc aatcacatct gtattttcca
    11461 gcggtttaac gccgcctcta ccatccagca tccaggcaaa tactgcatcc gggacattaa
    11521 cgtccgatcc cttaatcgct tccacagtgc ctccatcatc aacgcattat tttgtagtct
    11581 agccttctgg ccctgttacg caacatctca tcaccccatt accctgaaat gattaataaa
    11641 attctgtcta aattgaatac aaaaagcaaa atgcttttcc gtatacaaac cgtgtgaagt
    11701 gttaaatagc gtctatcatt atcagaatta tctgatcata tgacgtggct tttttgcgat
    11761 cggatagcaa caaaaattga taaaaataat gggatctcaa tgattacgca caacttcaat
    11821 actctggact tactcaccag tcctgtctgg atcgtttcgc cctttgagga acagttaatt
    11881 tatgccaaca gcgcggcgcg actgttgatg caagacctca cgtttagtca gctacgaacc
    11941 ggtccctatt ccgtctcctc acaaaaagaa ctgccgaaat acctctccga tctgcaaaac
    12001 caacacgata ttatcgaaat cctcactgtt cagcgtaaag aagaggaaac agcattaagc
    12061 tgtcggcttg ttttgcgaga gctgacagaa acagaaccgg tgattatttt cgaaggtatc
    12121 gaagcgccgg caacgctggg tttaaaagcc agtcgctcgg caaattatca gcgcaaaaaa
    12181 caaggttttt atgcgcgctt ttttctgact aactctgcac caatgttgtt gattgacccg
    12241 tcacgagatg gacaaatcgt cgatgctaac ctcgccgcgc tcaatttcta tggttataac
    12301 catgaaacga tgtgccagaa acatacctgg gaaataaata tgctcgggcg tcgcgtcatg
    12361 cctatcatgc atgaaatctc gcatttaccc ggtggtcata aacctctgaa ttttattcat
    12421 aagctggcgg atggttcgac tcgtcatgtg cagacctatg ccggaccgat tgaaatttat
    12481 ggcgacaagc tcatgttatg tattgtgcat gatattactg agcaaaaacg gctggaggag
    12541 cagctggaac atgctgctca ccatgacgcg atgaccggat tactgaatcg gcgacagttt
    12601 tatcacatta cggaaccagg ccaaatgcag cacctcgcca tcgctcagga ttacagctta
    12661 ttgctcatcg acacagatcg ttttaaacac attaacgatc tctatgggca ttctaaaggt
    12721 gatgaggtgt tatgcgccct cgcccgcact ctcgaaagtt gcgctcgcaa aggcgatttg
    12781 gtgtttcgtt ggggaggcga agagtttgtc ttattactac caagaacccc actggatacc
    12841 gcgctttcgc tggctgaaac tatccgcgta agcgtggcaa aagtgagtat ttcgggctta
    12901 ccacgcttta ccgtcagcat tggtgtggcg catcacgaag gaaatgaaag catcgatgaa
    12961 ctgtttaaac gcgttgatga tgctttgtat cgggcgaaaa atgatggacg caaccgcgtg
    13021 ctggcggcat aagccacgga tgcgtctcga gatcaacgac tgcgcttagc gtggcgctcc
    13081 cagttttctt gctttgcctg tgccgtttta cgtagtgcga cgtaacacgc cccgctgccg
    13141 ccatgatgcg gtagcgcggt acaatatgcc tggacatcat caaattcggt caaccagcgc
    13201 gccacatagc tgcggacaat attggcatgc gatttatcat cccgcccttt accatgaata
    13261 atcagcacgt tacgcaaacc atccgccatg gcttgttgaa tgaaactgaa catcattttg
    13321 cggcactctt ccaccggctg gcgcaaaaga ttcaggctcg cctgttgcgg atatttacca
    13381 ctgcgcagct tatccagcac cccatgttgc aacccttccc gccgaaactc cagcggctga
    13441 cttagcggga tgatgtcgag aaatccggtg gtgaggaaat tatcaagttg cagcgtgtcg
    13501 atacgctgcg gcgcacgttg gttacgcgtt ggatgccagt ggacatcggt agcacgtttc
    13561 agcggctgga catcttccat ggcgtcaaga aacagcgatt tgtcgtcaag gttcatgtta
    13621 catcctcccg caattaagag cgcgatatga taaccagacc gggtcggtcc aacaacgtat
    13681 tacccaaaat tccagtaata agttccaaat attgccgata ttttaagcaa aatgcttatg
    13741 catgattatt cattcacgat attaataatg taacttatat tttcgtgaaa tctgtcactg
    13801 aagaaaattg gcaactaaag gttaaaaccg ttataacaca gtcaccggcg cagaggagac
    13861 aatgccggat ttaagacgcg gatgcactgc tgtgtgtact gtagagtctg gcggatgtcg
    13921 acagactcta tttttttatg cagttttaac tttgcagata gccgcattct cgccggaatt
    13981 gcgtgattaa tgacgcggtc agtggtgtct ggcgactatc acgccgctga atcaaataat
    14041 aggccgcttt cggtaaaatt tcgctaaccg gcaacatcac cagcccctgt ccgtgcaagg
    14101 gatcgcagcc catttcttca ggcagtatgc tgagaaaatc gctttttgcc accagactga
    14161 tacaggctga gaacgtctcg cagactacac cgacctgtgg cgtttgcgcc tgatcgtcaa
    14221 gcaattcact caactgtttg tagtagctgc cgtgtggcgt cggcattgtc cagctgtaat
    14281 ccagtaactg tttgatcgaa cgggcaccaa tggcggggtg tcccgggcgg caaaagatcg
    14341 cgaattgctt ttccagtaat ttctcaaaag taaattcgtg gtcgtacggt ccctgataat
    14401 aggtattgat ggtgaaatcc aattctccct gacgcaattc attaatcatc gacaccagtt
    14461 gcccttccat aatgcgtact tttacctgcg gatgctgctg atgaaaacga gatattacag
    14521 ctggcatcag actgcgggaa atactggccc ccatgccgat attaatctgc cctgccagtt
    14581 gcccttgtcg ttggcgaata tcctcttggg ctgcgcgcag ttcttcaaga ataagactgg
    14641 cgtgctgata aaaactttca ccggcatcag ttaacgtcac gcctttacta cggcgaaaaa
    14701 agagttgcgc cgctaaccct tcttccagct cctgaataga tttactcagt gccggttgcg
    14761 acatattcaa cattcggctc gctccgcgaa tgctgccctg acgagccact tcaacaaaag
    14821 cccgaatttg atgaattttt acctgaaaag ccatgacgcc accgataacc gttatttatc
    14881 agaccaaaga aactggcatc tactttaatg cagatgattg tgtcagggta atttatgaac
    14941 ggttaaaact gtgaaaaatc agttagtgat aagtaaaaac tatcgctacg tgaaccgggt
    15001 cacacttttt actgatgacg ggaaaggtta tggagtcttt gaatcaattt gttaattcgc
    15061 ttgccccaaa attatcgcac tggcgacgtg attttcatca ctatgcagag tctggctggg
    15121 tggaattccg cactgccacc cttgttgcgg aagaattgca gcagctcggc tactcactgg
    15181 cgctgggccg cgaagtagtt aatgaaaata gccggatggg actacctgat gaactcactc
    15241 tacaacgcga attcgagcgc gctcgtcaac agggtgcgct agcacaatgg attgcggctt
    15301 ttgaaggtgg tttcacgggt atcgtcgcca ccctggatac cggtcgcccc ggtccggtga
    15361 tggctttccg tgtcgatatg gacgcgctgg atctcagtga agagcaggat gtcagccatc
    15421 gcccctaccg cgacggtttt gcgtcatgta acgccggaat gatgcatgcc tgtggtcatg
    15481 atgggcatac caccattggg cttgggctgg cgcataccct taaacaattc gagtccggac
    15541 tacatggcgt catcaaactg atttttcagc ctgcagagga aggtacgcgt ggcgcgcggg
    15601 cgatggtcga tgcaggtgtc gtagatgatg ttgattattt tactgccgtg cacattggca
    15661 ctggcgtacc tgcgggcacc gtggtgtgcg gcagtgataa ttttatggca accaccaaat
    15721 ttgacgcgca cttcaccggt accgccgctc acgcaggcgc aaaaccagaa gacggttaca
    15781 atgccttgtt ggcggcagca caagccactc ttgcactgca tgcaatcgcc ccgcacagcg
    15841 aaggagcttc cagagtaaac gtgggcgtta tgcaggcagg aagcggtcgt aacgttgttc
    15901 ctgcctcggc gttgctgaaa gtggaaacac gcggggccag cgacgtcatt aatcaatatg
    15961 tttttgaccg tgctcagcaa gcgattcagg gcgcagcaac catgtatggt gtcggcgttg
    16021 aaactcgtct gatgggtgca gctaccgcca gttctccttc gccgcaatgg gtcgcatggt
    16081 tgcaaagtca ggcggctcag gtcgcggggg tcaatcaggc cattgaacgt gttgaagcgc
    16141 ctgcgggttc cgaagatgcc acattaatga tggcccgcgt gcagcaacat caagggcaag
    16201 cctcctacgt ggtgtttggc acacagctgg cggcaggtca tcacaacgaa aaattcgatt
    16261 ttgacgagca ggttctcgct attgccgtcg aaacgctggc gcgcaccgcg ctcaattttc
    16321 cctggacgcg aggtatctga tgcaggaaat ctatcgtttt atcgacgatg cgattgaagc
    16381 cgatcgccaa cgttataccg atattgccga tcaaatctgg gatcatccag aaacacgttt
    16441 tgaagagttc tggtcagcgg agcatctggc ttcggcgctg gaatctgcag gcttcaccgt
    16501 tacccgcaac gtaggcaata tcccaaatgc ctttattgct tcgtttggtc aaggcaaacc
    16561 ggttatcgcc ctgctgggag aatatgacgc cctggcaggt ttaagtcagc aagcaggttg
    16621 cgcgcaacct acatccgtga cgcccggtga aaatggtcac ggttgcggac acaatttgct
    16681 gggaaccgcc gcctttgccg ctgcaatagc catcaagaaa tggctggaac aatatgggca
    16741 aggcggcaca gtgcgctttt atggttgtcc tggcgaagaa ggcggctcgg gtaaaacgtt
    16801 catggtccgc gagggggtat ttgatgatgt ggatgcggca ctcacctggc acccggaagc
    16861 ctttgccggt atgttcaata cccgtacgct ggcaaacatt caggcatcat ggcgctttaa
    16921 agggatcgca gcacatgccg cgaattcccc tcatttggga cgcagcgccc ttgatgccgt
    16981 aacgttgatg accactggca ccaacttcct caacgaacat attattgaaa aagcgcgcgt
    17041 acactatgcc atcacaaata gtggcgggat ctcgcccaac gtggtccagg cgcaggcaga
    17101 agtgctttat cttatccgcg cccccgaaat gaccgacgtg cagcatattt atgatcgggt
    17161 cgccaaaatc gccgaaggtg cggcattgat gaccgaaacc acggttgaat gccgcttcga
    17221 caaagcctgt tccagttatc tcccgaatcg caccttagaa aatgccatgt accaggccct
    17281 atcccatttt ggtaccccgg aatggaactc cgaagaactg gcttttgcga aacaaattca
    17341 ggctacgctt acctccaacg atcggcaaaa cagtctgaat aatatcgccg caaccggtgg
    17401 cgaaaacggc aaggtttttg cactacgtca tcgtgaaacg gtactggcga atgaagtcgc
    17461 tccatatgcc gccaccgata acgtgcttgc ggcatcgact gatgtcggcg acgtcagttg
    17521 gaaactgcct gttgcccagt gtttcagccc ctgttttgcc gtcggtacac ccctacatac
    17581 gtggcaactg gttagccagg ggcgaacatc tattgctcat aaaggaatgc tgctggcggc
    17641 gaaaactatg gcagcaacca cactcaatct cttcattgat tcagggctat tgcaagaatg
    17701 ccaacaagag catcagcaag ttacggacac gcaaccgtat cactgcccta tcccgaaaaa
    17761 cgtgacaccg tcacctttaa aataacaaca acaacgcaaa cacaacaacc gaggaatgcc
    17821 catgagtatg tcatccatac cgtcgtcctc ccaatccggg aagctctatg gctgggtcga
    17881 aagaattggt aacaaggttc cccatccttt ttgctcttta tctatttgat tatcgtactc
    17941 atggtgacga cggcaatttt gtcggccttt ggcgtcagtg cgaaaaaccc gaccgatggt
    18001 acgccggtgg tggtgaaaaa cctgctcagt gtggaaggat tacactggtt tttacccaat
    18061 gttattaaaa actttagcgg ttttgctcca cttggtgcga tcctggcgct ggttttaggt
    18121 gccggtctgg cggagcgcgt cggcttactg ccagcactaa tggttaaaat ggcatcgcat
    18181 gttaatgccc gctacgccag ttatatggtg ctgtttattg cttttttcag ccacatttct
    18241 tccgatgcgg cgttagtgat catgccaccg atgggtgcgc tgatttttct ggcggtgggc
    18301 aggcatccag ttgcaggttt actggctgcc attgcaggcg taggttgcgg ctttacggct
    18361 aatttactga ttgtcacaac cgacgtgttg ctgtcgggga tcagcacgga agcggcagct
    18421 gcgttcaatc cgcaaatgca cgtcagtgta attgataact ggtattttat ggccagctcc
    18481 gtagtcgtac tgacgattgt tggcggcctg ataaccgaca aaatcatcga gccacggtta
    18541 ggtcaatggc agggaaacag cgatgagaaa ctgcagacat tgaccga
//
LOCUS       DAZKIF010000097        18422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZKIF010000097 DAZKIF010000000
VERSION     DAZKIF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.097"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..486)
                     /locus_tag="ACHOTW_003329"
     CDS             complement(<1..486)
                     /locus_tag="ACHOTW_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845378.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QT"
     gene            complement(1113..1538)
                     /gene="arsC"
                     /locus_tag="ACHOTW_003330"
     CDS             complement(1113..1538)
                     /gene="arsC"
                     /locus_tag="ACHOTW_003330"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP9845379.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(1551..2840)
                     /gene="arsB"
                     /locus_tag="ACHOTW_003331"
     CDS             complement(1551..2840)
                     /gene="arsB"
                     /locus_tag="ACHOTW_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP9845380.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2894..3247)
                     /gene="arsR"
                     /locus_tag="ACHOTW_003332"
     CDS             complement(2894..3247)
                     /gene="arsR"
                     /locus_tag="ACHOTW_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP9845381.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3991..4074
                     /gene="dinQ"
                     /locus_tag="ACHOTW_003333"
     CDS             3991..4074
                     /gene="dinQ"
                     /locus_tag="ACHOTW_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP9845382.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4128..5480)
                     /gene="gorA"
                     /locus_tag="ACHOTW_003334"
     CDS             complement(4128..5480)
                     /gene="gorA"
                     /locus_tag="ACHOTW_003334"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9845383.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5552..6394)
                     /gene="rlmJ"
                     /locus_tag="ACHOTW_003335"
     CDS             complement(5552..6394)
                     /gene="rlmJ"
                     /locus_tag="ACHOTW_003335"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP9845384.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6597..8639
                     /gene="prlC"
                     /locus_tag="ACHOTW_003336"
     CDS             6597..8639
                     /gene="prlC"
                     /locus_tag="ACHOTW_003336"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP9845385.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8647..9399
                     /gene="rsmJ"
                     /locus_tag="ACHOTW_003337"
     CDS             8647..9399
                     /gene="rsmJ"
                     /locus_tag="ACHOTW_003337"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP9845386.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9448..10917)
                     /gene="dtpB"
                     /locus_tag="ACHOTW_003338"
     CDS             complement(9448..10917)
                     /gene="dtpB"
                     /locus_tag="ACHOTW_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP9845387.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11083..11136
                     /locus_tag="ACHOTW_003339"
     CDS             11083..11136
                     /locus_tag="ACHOTW_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845388.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            11114..11167
                     /locus_tag="ACHOTW_003340"
     CDS             11114..11167
                     /locus_tag="ACHOTW_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845389.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(11234..11668)
                     /gene="uspA"
                     /locus_tag="ACHOTW_003341"
     CDS             complement(11234..11668)
                     /gene="uspA"
                     /locus_tag="ACHOTW_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP9845390.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12059..12394
                     /gene="uspB"
                     /locus_tag="ACHOTW_003342"
     CDS             12059..12394
                     /gene="uspB"
                     /locus_tag="ACHOTW_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP9845391.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12465..13964)
                     /gene="pitA"
                     /locus_tag="ACHOTW_003343"
     CDS             complement(12465..13964)
                     /gene="pitA"
                     /locus_tag="ACHOTW_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP9845392.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14196..15398
                     /gene="yhiN"
                     /locus_tag="ACHOTW_003344"
     CDS             14196..15398
                     /gene="yhiN"
                     /locus_tag="ACHOTW_003344"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9845393.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(15716..16768)
                     /gene="yhiM"
                     /locus_tag="ACHOTW_003345"
     CDS             complement(15716..16768)
                     /gene="yhiM"
                     /locus_tag="ACHOTW_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFP9845394.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            17152..>18422
                     /locus_tag="ACHOTW_003346"
     CDS             17152..>18422
                     /locus_tag="ACHOTW_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9845395.1"
                     /translation="MKVFHNIFKYISSNHQDKHSDKVNNHQHHGKVDKKHRSEIVEID
                     KLDKHSQIDNDFGLHIIYFLQHGCWNVTEHSPQIEKIWFYNSEPSIDIKEYNSFADNT
                     TDTFIFTIIPDNNHVIKLSSPITVTIERKDGYYFINFSGDKSDIIYKVDGFSIKERNF
                     FTLLSGNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYALLVSGHIGIREKGWSLLCDLLKHEDMASFAYENKKLKKLFTLLDKRD
                     MILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVVVLAGNHEINFNGNYSARLANHKLSSGDTYNLIKTLDVCNYDSERHVLTSHHGI
                     IRDEENKCYCLGALQVPFNQMKNPTDPEELA"
BASE COUNT         4872 a         4437 c         4625 g         4488 t
ORIGIN      
        1 ggtttggaca ctgaatctta ctggtattcc ctcttctacg ctaattttag ttatgattgc
       61 cttgtcgggt ttaggattag cgacaacaat gattcgacat tcagcacttt tgttcttttc
      121 gtatctaatg ttattaattt gattaagtat ttcctgtgcc tgccgcgtaa gtggtgtcga
      181 acttatcgat tcaatgttag taataagact tacaagttga gccacaactt ttgagtagtc
      241 tgcatctgta tatttggtgt tcgtgcggca cgcataaatt tgtgggaggt gttgccgcgc
      301 taaatcatat tgttcagggt tgtatccttc tccagagaat gtggaattaa tgggttgttg
      361 ttcttcccga ttatcacaag gccacatcgg tgtgtctggt gttttaacat tgtcggtatc
      421 tgttgtgcgg ttataaatat cgccgtgata tgtatttatc atacctgggg taaagtcaat
      481 tgacataaca ttccttattc attatctgtt ttatgctaag atttttaaat tggataatta
      541 aagcaggaat ggattttctt ctacgcacaa taaatatcat gcgtcatatc ttcctgatat
      601 aattaccttt gacttccctt ttatctccac ccggtaatat tcgtgtataa aaaagggcat
      661 tgatagtatc gtaaaggaaa acgttaacaa ctatcaaaaa gcgctcattt taaaaagtta
      721 tgatgttctc cgatagtgtg tgaaatgata tttttgtgat gttttagatg tgtaataata
      781 aaatgggtta aggtatctta taattattga ataatttgtt gtgtaaagca ctgataaatc
      841 ataatccagt acgtttttaa actgctgcac tgtatcgctg atcactaagc agctcactga
      901 tacatcatgc tggacgcgat aattggaatc tcacgcaggg agtaatgtgt aaatagcaaa
      961 atcgcctgat gcgctacgcc tgtcaggcct acaaataaca tacaatttat tgaattttca
     1021 tgactttgta gatcggataa gacgcgttag cgtcgcatcc gacataaata aagcgcactt
     1081 ttctaacaac ctgtgggggg gatatagccg ctttatttca ggcgcttacc cgcttcatca
     1141 accactttct cgccatcttc cttggtgaat gcgccttttt gcgcatctgg cagaatttcc
     1201 agcaccactt ctgaagggcg gcacaggcga gttcccagcg tcgtcaccac aatcgggcga
     1261 ttaatcagaa tcgggtgctg aagcataaag tcgattaacc gatcgtcagt aaatttatct
     1321 tccgcaaggc ccagctcctc atacggttcg acgtttttac gcagcagcgc gcgtacggaa
     1381 atccccatat cggcaatgag tttgaccagt tcatcgcgcg ttggcggagt ttccagataa
     1441 tggataatgg tcggttctgt gccgctgttg cggatcatct ccagcgtatt acgcgacgtg
     1501 ccgcaggccg ggttgtgata aatggtaatg ttgctcatat cagtatctca ttacaaagtg
     1561 aaagagagac gtagcgccag cgcagccagc gtcacaaaca gcacaggcag ggtcatgata
     1621 atccctgtac ggaagtaata tccccagctg atagtcatat tcttctgcga aagtacgtgc
     1681 agccagagca gcgttgccag gctaccaatc ggggtgattt tcggccccaa atcgcagcca
     1741 atcacgttgg cataaaccat cgcttctttg ataacgccag atgccgtgct gccatcaatg
     1801 gacaacgcgc caaccagtac cgtcggcata ttgttcataa tagaagagag gaaggcggtg
     1861 aggaatccgg tgccgagcgt cgcggcccac aggccgttat ccgccagcac gttgagtacg
     1921 ccagaaagat attccgttaa tccggcattg cgcaggccat aaaccaccag atacatgccg
     1981 agcgagaaga tgacaatctg ccagggcgca ccgcgcagca ctttacccgt attaatcgca
     2041 tgaccgcgtt tagcgacgac aaataatatc aacgcgccca ctgccgcaat ggcgctgacc
     2101 ggaatgccga gcggttccag gacgaaaaat cccaccagca gaagcagtaa aacaacccag
     2161 ccagttttga acgtagcagg atctttaatc gcttctgcgg gagatttcag cagcgccatg
     2221 tcgtagttct gcggaatatc tttgcggaaa tagagatgca acatcaccag cgtggcaaca
     2281 atcgcggcga tatccaccgg caccatcacc gaggcatatt cgcgaaagcc gaggccaaag
     2341 aaatctgcgg aaacgatatt caccaggttg gagacaataa gcggcaggct ggcggtatcg
     2401 gcaatgaatc cggccgccat cacaaacgcc agcgtagtgc ctttactgaa ccctaaagcc
     2461 agcagcatgg cgatgacaat cggtgtcaaa ataagcgccg cgccatcatt ggcaaacagg
     2521 gcggcaacgg cagcaccgag caggacaatc caggtaaaca gcaagcgacc gcgtccattg
     2581 ccccagcgtg aaacgtgcag tgccgcccat tcaaaaaagc cggactcatc cagcagcagg
     2641 ctgatgataa taacggcaat aaacgcagcc gtcgcgttcc agacgatatt ccacaccacc
     2701 ggaatatcgc ccggatgtac tacgcccgta actaacgcca gtactgcgcc gagcgttgca
     2761 ctccagccga tgcctaaacc tttcggctgc cagataacca atacaatggt caggacaaag
     2821 atagcgcctg ccagtaacat aatgcctccc ggaaaaacac atgtgcaaat ccatatgtgt
     2881 ttagataaat tttttaacta caagtgttct tactgtctac ggaacaattt tgtctggcca
     2941 ggttgcggac aatcacctga accttttcct gttcgcatcg ccaggcctgc tcaataattt
     3001 tcgcggccca tgatggaata tgcggtgata agcggtaatg aacccactta ccctgcttgc
     3061 gatccagcaa caaaccgctt tcacgaagaa gcgccagatg gcgggagatc ttgggctgcg
     3121 actggtcgag agcagtgcag agatcacaga cgcataactc tcccagctcg ctgagcagta
     3181 aaacgatgcc cagacgggtt tcatcggcaa gaattttgaa cagttggatg ggtaacagaa
     3241 atgacatatt gcgctcctga ttgttgcagg tagtgtctct cttcgaagcg gataagtcaa
     3301 aaacatatat ggtttaacga atgtgtatgt gcagaggaag gtaataggtg tgaattaagc
     3361 gttggctatt catttgaaag gaggtctgaa tcaggacgta aaaaaaccga ctttgcgtcg
     3421 gttttttact ttccagccca gagttggtgg ctctggctgg agtgagaatg cccacatcta
     3481 acggttcaat actattagcc agtggctaac attgcaagcg ttagcgattt cttcagctgg
     3541 agaagggaca gtttgctata catgttggtg aaagaggatt tatatacttt catcgggtcg
     3601 ctgccgtacc aggctttgtc cgaatccgga tgtaaaaaaa ccgacttcgc gtcggttttt
     3661 ttactcttca gccctgagtt ggtggctctg gctggagtga gaatgccatc atctaacgca
     3721 tcaatactaa taccatactg aaactattgc aaggacatgc tggttttata acctgaatat
     3781 actgtatgat tatccagtta gctctgaggc attttcactc tggcaatgcg cataaacgct
     3841 ttcaaagtcc tgttcagtgg tacgggtggt gccgttaact gatgctctgg ccggagtgag
     3901 aataccatta tctaacaata gggcatgcgc cgtgacaggc gatggatgag taagcggatg
     3961 cattctcact ccatcgtatg gagaacacgg gtgattgata aagcaatcat cgttcttggg
     4021 gcgttaattg cgctgctgga actgatccgc tttctgcttc agcttctgaa ctgatagcgg
     4081 aaacgtaatt aagggcaaag agcacaccac tctttgccct ttaacattta acgcatggtc
     4141 acgaactctt ctgccgccgt tgggtgaatg gcgacggtat tatcgaagtc ttttttggtt
     4201 gcgcccatct tcagtgccac cgcgaagccc tgcaacattt cgtccatacc aaaaccaatg
     4261 ccgtgaatac cgacaatctt ctcttctggc ccaacgcaca ccagcttcat gcggcacggc
     4321 tggcggtgag tggtgacggc ggtatacatc gcggtgaaag aggatttata cactttcacc
     4381 tgatcgtcgc cgtactgctc gcgcgcctgc ggttccgtta aaccaacagt accaatcggc
     4441 ggatggctga agaccacggt cggaatgttg ctgtaatcca gatgctcatc cggcttgtta
     4501 ttaaacaggc gttcagagag acggcgaccc gctgcaactg ccaccggtgt cagttctact
     4561 gcacccgtgt tatcgcccac cgcgtaaata ccttcaacgt tggtgttttg atatttatcg
     4621 acgacgatat agcctttttc gttggtttta acgccagcgg cttcgaggtt gatgttgtca
     4681 ttggcaggct cgcggccaat cgcccaaatc aggcaatcca ccgtttcact gcgaccatct
     4741 tccagctcca gcgtcaggct accatcggca tttttcacta ccgctttcgg gatggcgttg
     4801 gtgtgcaact gcgggccttc ggcgttcatc acttcgacca gcgtttcaga gatcatcggg
     4861 tcgaagctgc gcagcggcgc atgtttacgc acaaacagat gcgttttcgc gccgaggccg
     4921 ttaatcacgc cagccagctc aacggcgatg taacccgcgc caacaaccgc cacacgctct
     4981 ggcaaagcag gaagggcaaa gaaaccatca gaatcaatac cgtattccac gcccggaata
     5041 tccgggtggc tcggacgacc gccggtggcg atcagaatat gatcggctgt gatggtttca
     5101 ccgttaactt ccagcgtttt ggcatcaacg aagcgggcaa agcctttgat tacatcaacg
     5161 ttatttttac cgagcacgtt ttcataggaa gtatgaatac ggtcgatata ggcggtacgg
     5221 ctggcgatca acgtttccca gttgaattta ttgatagtgg tatcaaaacc ataatccggg
     5281 ccgtacatat ggatcgcttc acggatttgc gccgcgtgcc acatcacttt cttcggtacg
     5341 cagccgacgt tcacgcaggt gccgcccagc tctttggctt caatcagcgc gcatttctga
     5401 ccgtacatag ccgcgcggtt gatggaggcg ataccgccgc tgccgccgcc gatggcgatg
     5461 taatcatagt gtttagtcat gacagagtgt ccttatcgtt gattaccgcg attgtagcgc
     5521 gagacgtaat aggtgccagc aatggctgca attactctgg cacgatccag cttacggtgg
     5581 cgtgcccggt gccagctgga accagtttgc tgtgcagcca cggcagcacg ttattcatct
     5641 gctgttccag tttccacggc gggttaatca caatcatgcc ggaagcggtc atgccacggc
     5701 gatcgctgtc tggcagtacc gccagttcaa tttgcagaat tttgcgaatg ccggtcgctt
     5761 ccaggtcgtg gatcatgcgc ttaatttgct ggcgcagcac caccggatac cacaatgcgt
     5821 aagtgccagt ggcgaaacgt ttgtaacctt ctgctatccc gctgactacc gcctgatagt
     5881 cggttttcat ttcatacggc ggatcgataa ggattaaacc acggcgagaa accggcggca
     5941 gtttggcctt aagctgctgg aaaccgtcgg atttttcgac gcgcgcacgg ctgtctttct
     6001 gaaattcaga acgcagcaac gggtaatcgc tcgggtgcag ttcggtcagt tgcaggctgt
     6061 cctgttcacg cagtaactga cgggcaatca acggtgaacc ggggtagtaa cgcaactgac
     6121 cgctacggtt gaagtgtttt accacattga tgtacggctc cagctccgca ggcaaatcgt
     6181 cctgctgcca gatacgggcg ataccttcga gatattcgcc ggtacgctcg gcatgttcgc
     6241 tgcctaactg ataacgcccg gcccctgcgt gggtgtcgag atagagaaac ggtttatctt
     6301 tctctttcag cgactcgatg atcaggctct gaacggtatg tttaaggacg tcggcgtggt
     6361 tgccagcgtg aaagctgtgg cgataactga gcatgggtaa aggtgttccg gtaagtaaaa
     6421 atcggcccgt attcggggcg cacaaaagcc tatcaggaca gtataccgaa aagaggccgc
     6481 cgccgcgaaa gcgtaacgtt tctcattgaa attcactaca cttaacccca tgctacaaac
     6541 attatgtaaa gcgcctgttg agcgcttcct taacctcttt aaccaggact gcgctaatga
     6601 cgaatccgtt actgactccc tttgaattgc ctccgttttc taaaattctc ccggaacatg
     6661 tcgttccagc cgtgactaag gcattgaatg actgccgcga aaatgtggag cgcgtagtag
     6721 cgcaaggagc accgtacacc tgggaaaatc tctgccagcc gttggcggaa gtggacgatg
     6781 tgttggggcg tattttctcc ccggtcagcc acctgaactc ggtgaaaaat agcccggaat
     6841 tgcgcgaagc gtacgaacaa actctgccgc tgctgtcgga atacagcacc tgggtagggc
     6901 aacatgaagg gctgtataaa gcgtaccgcg acctgcgcga tggcgatcat tacgccacgc
     6961 tgaacacggc tcagaaaaaa gcggttgata acgcactgcg cgattttgaa ctctcaggca
     7021 tcggtctgcc gaaagagaaa caacagcgtt acggcgaaat tgccactcgt ctttctgaac
     7081 tgggcaacca gtacagcaac aacgtccttg atgcgacgat gggctggact aaactcgtta
     7141 ccgacgaagc ggaactggcg gggatgccag aaagcgcgct ggctgcggca aaagcccagg
     7201 ccgaagcgaa agagctggaa ggctacctgt tgacgctgga tatcccaagc tacttgccgg
     7261 taatgaccta ctgcgacaac caggtcttgc gtgaagagat gtatcgtgct tacagcaccc
     7321 gcgcttcaga tcaaggtcca aacgccggta aatgggataa cagtaaggtg atggaagaga
     7381 tcctcgcgct gcgtcacgaa ctggcgcaac tgctgggctt tgaaaactac gcgtttaaat
     7441 cactggctac caaaatggcg gaaaacccgc agcaggtgct tgatttctta accgatctgg
     7501 caaaacgcgc gcgtccgcag ggcgaaaaag agctggcgca actgcgtgcc ttcgccaaag
     7561 ccgaatttgg cgtggatgag ttgcaaccgt gggatatcgc atactacagc gaaaaacaaa
     7621 aacagcacct ctacagcatc agcgacgaac aactgcgtcc gtacttcccg gaaaacaaag
     7681 cggttaacgg cttgtttgaa gtggtaaaac gtatttacgg catcaccgct aaagagcgta
     7741 aagatgttga tgtctggcac ccggatgtac gtttcttcga actgtatgac gagaacaacg
     7801 aactgcgcgg cagtttctac ctcgacctgt atgcccgtga aaacaagcgc ggcggggcgt
     7861 ggatggatga ctgcgtaggc cagatgcgta aagccgatgg ttcgctgcaa aaaccggtcg
     7921 cgtatctgac ctgtaacttc aaccgcccgg tgaatggtaa accggcgctg ttcactcacg
     7981 acgaagtgat cactctgttc cacgagttcg gtcacggcct gcaccatatg ctgacccgca
     8041 tcgaaaccgc cggtgtttcc ggtatcagcg gtgtgccgtg ggatgcggtc gaactgccga
     8101 gtcagtttat ggaaaactgg tgctgggagc cggaggcgct ggcgtttatc tctggtcact
     8161 acgaaaccgg cgaaccgctg ccgaaagagt tgctggataa aatgctggcg gcgaagaact
     8221 accaggcggc gctgtttatt ctgcgccagc tggagttcgg cctgttcgat ttccgcctcc
     8281 atgccgagtt ccgcccggat cagggagcga aaatcctcga aactctggca gaaatcaaga
     8341 aactggttgc cgtagtaccg tctccatcct ggggccgttt cccgcacgct ttcagccata
     8401 ttttcgccgg tggttatgcc gcaggttact acagctacct gtgggctgac gtactggcgg
     8461 cagatgcttt ctcgcgcttt gaggaagagg gcattttcaa ccgtgaaacc gggcagtcgt
     8521 tcctcgacaa cattctgagc cgtggcggtt cagaagagcc gatggatctg ttcaaacgct
     8581 tccgtggtcg tgaaccgcag ctggatgcga tgctggagca ttacggcatt aagggctgat
     8641 cattcagtga aaatctgctt aattgatgaa acaggcaccg gagacggtgc cttatctgtt
     8701 ctggcggccc gctgggggct ggagcacgat gaagacaacc tgatggcgct ggtgttaacg
     8761 ccggaacatc tggaattgcg caagcgtgat gagccaaaac ttggcggcat ctttgttgat
     8821 tttgttggcg gagcgatggc gcaccgacgc aaattcggcg gtggtcgcgg tgaggcggtg
     8881 gcgaaagcgg tgggcattaa aggcgattat ttgccggatg tggtggacgc taccgcaggg
     8941 ctggggcgcg atgcctttgt gctggcttcg gtcggctgcc gcgtgcggat gctggagcgt
     9001 aatccagtcg ttgccgcgct actcgacgac ggcctggcgc gtggatatgc ggatgcggaa
     9061 atcggcggct ggttgcagga gcgtttgcag ttaattcacg cctccagcct gacggcgctg
     9121 accgatatta ccccgcgtcc gcaggtggtc tatctcgacc cgatgttccc gcataagcag
     9181 aaaagcgcgc tggtgaaaaa agagatgcgc gtctttcagt cgctggtggg accggatctt
     9241 gatgccgatg gattactgga gcctgcacgc ttgctggcga ccaaacgcgt ggtggtaaag
     9301 cgcccggact acgcgccacc gctagcgaat gtcgccacgc caaacgcggt tgtgactaaa
     9361 gggcatcgct ttgatatcta tgcaggtacg ccagtgtaaa agtaaagcag ggtagcgata
     9421 acgctaccct gctttctgca agaataatta atggctttcc ggcgtcgcaa tcatgcgttt
     9481 cagccacggc accatcaaca gcatcactac tgcaacgccc agcgtgacca gaccaatctt
     9541 accaaacacg ttggtataga caggcaacgt ctcaagcgga tcggtaatgt tgtctggtac
     9601 tgcggtaaat gttgccacat agccgcccag caagaacgcg gcagcctgtg tcaggaacca
     9661 catcccgaga ataaagccca tcaaatgctg cggcaccaga gcagcaatca tcgccaggcc
     9721 aagggcgcta ataaacagtt cacctaagct ctggaataag tacaccagca cgataaacca
     9781 tggcgatgtc agcccttgtg catccgcaaa ccacattccc gcagctgccg ccgtcaaaaa
     9841 gcccagtgag cacataaaca tgccgagagt aaatttcatc ggcatcgaga ggtctttgcc
     9901 tttgttaccc agatgcgtgt aaatgcctgc cagtattggg ctggcgagta ccacccagaa
     9961 cgggttaagc gcctggaagc tgaccgggtt gatggaaaaa ccgagaattt catgatgcac
    10021 gttgttgatg gcaaagaagt tcagcgatgt tggcatctgg gcgtagagaa tgtaaaacac
    10081 caccgcttcg agcatcagga caaaggcgac aaacatttta ttgcgcccgg ttttatccag
    10141 cttgaatgcc tgacgaaaga agatgatggt gacgacgatg gagagaacaa tcagcaccag
    10201 attggcgact tctacgttgt gcatcagcca ggcgcagacg aagatcatca ccacgctgcc
    10261 aagtaatacg tacaacagtt tgctgaagct cattggccgg aagtcgggct cagaaccaat
    10321 gtctttcacc attccacgac aggcgatgta aaccagtaat gcgataatta accccgcacc
    10381 gcacaggttg taggtgactg aatagccgaa tttatcagcg atcacagggg ccagcgataa
    10441 cgctatcaac gagccgatat tgatcgacat atagaacagg gtgaatgtgc catcaaggcg
    10501 cggatctttc ggcggatagc acttcgaaag caagctggct gggttagctt taaacaggcc
    10561 gttaccgaca gcgatagtcc ccagggcgat gaaaatcagg tcaggcttaa gtagcgacat
    10621 gccggtcatg aagtagccaa tcgccagcac aagtgctcca agaacgatgg tgcgtttggt
    10681 ccccagcagg tggtcgccga catagccgcc aatggaaatg aggccataga ccagtgcagc
    10741 aaaagcacca aaagtgacaa aagcctgctc ttgcgagaat ccaagctgtt taacgaagaa
    10801 aaccgccagt acgccctgca cgccgtagta gccgaatcgc tcccataact cgacaaaaaa
    10861 gatcatgaaa aatgggcgag gttgctgcag catccccatg ggtgttgttg tattcatatt
    10921 tattaacctt ccaataccat cctgaaaagt atgaacgcgt aagggataac gcgcgaaaaa
    10981 gtttacatct gtcagagatt cttaatattg atgcggtgaa tagattaata cactattgca
    11041 acagaaaata accagctgat cacactaatg atggaaaata tgatggtgtt aaatattgta
    11101 ttggctgggg attttgattg cgaacgcaga ccgtaggcca gataaggcgt ttacgctgat
    11161 caggtaacaa ggcgcgacaa aaagcaaaac gcccgcatat gcgggcgttc aggaacacgt
    11221 cgtggaggga agattattct tcttcgtcgc gcagcggaac aatcagcata tcaacgtgaa
    11281 cggtgttgat cagctgacgt gcggaagaca tcagtttgct ccagaagtcc tggtggtgac
    11341 cacaaaccac tagatccata tcgtatttct tgattgcatc gaccagaacc tggcccaggt
    11401 cgccgctgcc gctcagggtt tcagtgattg ggtagcctgc attagtggaa agctcggtca
    11461 gcgcatgatg tgtctcttca gagatgcgtt tctgcatatc accaagattc acatcaataa
    11521 gcccggtata taggtcagag tagtttacat ctacgtggat cagagaaact ttcgcattgt
    11581 atgggcgagc catagagact gctttctcta ccagaacttt gctttccggg gagagatcga
    11641 ccgcgatgag aatgtgttta taagccatag tgttactcct tccataaagt tgtcgatgac
    11701 tggccagcta gcgtttcttg tgccgttacg tcacccgcgt cctgcgaact acgtgcgcgg
    11761 ggctaaccca gccgtaaaga ttcagtgtta agaccatcct taccttatag cgacccggat
    11821 gatccgtcaa tccgccttgc ttacccgtta gataaacaaa ttttcgacat gaatatattg
    11881 atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct atagagccgc
    11941 tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact gattggcagc
    12001 gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg gaggaggtat
    12061 gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta acatggcgcg
    12121 ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc cattgctcta
    12181 tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca aacaggtgcg
    12241 tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg agtttattcg
    12301 ccgctgtgag cgggtgcgtc ggcagtttat tctgaccagc gcattgtgtg gtctggtggt
    12361 ggtcagcctg attgccttga tgatttggca ttaagcactt cagcaataat aacgcataaa
    12421 aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt actattacag gaactgcaag
    12481 gagagccagt aaagcccgcc ggaaagcagt accgcagccg gaagggtaaa cacccaggcc
    12541 atcagaatgc tggtcacggt tttacgctgt aagccgccac catctaccac catcgtcccc
    12601 gcgacagaag aggagagtac gtgagtagtg gaaaccggca tcccggtata actcgccagg
    12661 ccgatagaca ctgccgcagt catctgggca gacatcccct gagcgtaggt catgcctttc
    12721 ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat cgtaccgata
    12781 cctaacgcca gcgcgaccgc catgatgatc cacaccggtg catactcgat ggtgctaagc
    12841 atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacgcc aggcatcttc
    12901 accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg gctacgttga
    12961 tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt caggcggttg
    13021 agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt tgcgccagct
    13081 tccggagccg gtactaactg atcagcacca gtagcttgtt tcagcagcgc aggatgctgc
    13141 tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc gtagccagtg
    13201 gcattcatgt tcaccacgaa gcctgctggt gcgacaccaa tcaataccaa cataaccaga
    13261 ccaatgcctt tctgaccatc gtttgcgccg tgcgaaaacg ccacgccgat agcggaaagg
    13321 atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt ttcacgctcc
    13381 gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg acgcagcaag
    13441 aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag agaaccgaaa
    13501 atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt catcaacgca
    13561 ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga tgcaggcaaa
    13621 ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga gaacaccatg
    13681 gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat atgcacaatg
    13741 gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac cgctgccata
    13801 accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac ggcgttggct
    13861 gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc aagcaataat
    13921 aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg ttacgccatt
    13981 ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa agtgaaatat
    14041 agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta tcttatataa
    14101 ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga ggaatcttta
    14161 caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat gccattatta
    14221 taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggca ggacgccggg
    14281 ttctgctgat cgataatggt aaaaaaccag ggcgcaaaat ccttatgtct ggcggtgggc
    14341 gctgcaactt taccaacctt tatgtcgaac cgggcgctta tctgagccag aatccgcatt
    14401 tttgtaagtc tgcactcgcg cgttttaccc agtgggattt cattgatctg gtcaataaac
    14461 acggcatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac tccgcgcagc
    14521 agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtgaccgtc agattgcgta
    14581 gcgaagtgct gagtgtggcg aaggatgata caggcttcac gcttgaactg aacggcatga
    14641 ctgtcggttg tgaaaagctg gtcatcgcga ccggcgggct gtcaatgccg ggcctgggcg
    14701 cgtcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg ccgacccgcg
    14761 cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag gtgctggcgg
    14821 gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt gagaacttac
    14881 tcttcaccca tcgcggcttg tctggaccgg cggtgttgca aatttccagc tactggcaac
    14941 cgggggaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc ttcctgaatg
    15001 agcagcgtaa cgcacatccg aatcagagcc tgaaaaacac actggcggtt catctaccga
    15061 agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg ctaaaacagc
    15121 tcaacgtgcg tgaccaacag gcactgatta gcacattgac cgactggcgc gtacaaccca
    15181 acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac accaacgaac
    15241 tctcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtactttatc ggcgaagtga
    15301 tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg agtgcgtggg
    15361 cttgtgcgca ggatttgatt gcagcgaagt cgtgctgagc tatttttcct aaagatgaaa
    15421 attattagag tcgcattaaa aatgagctaa ttttgatagt ggctatcttg tgattatttt
    15481 ctaatgagcc cgtgaactga accccccatg cttaatataa ggtggaggga aaggtgattg
    15541 aaaactcacc cagtgggctg atctttataa aatatgaaca ggttgaaaaa aaaacagtaa
    15601 ctttgctgtt ttttatactt aaccactatg cattaatgct gcgttatttc attctaccta
    15661 agaaaaacca gcgttacgca aatggttaac gctggtttta tccggtacgt tgcaattatt
    15721 ttttagcaga acccgcttct aatattgaaa cgattgagaa caacgtaaag cataccgctc
    15781 ccaaaccgac cagaacccgc gaaggaataa aatatcccat atcggtctgc gccatttccg
    15841 caagaaacga tgctaggaaa aggcaaaaca ggcaggtgaa gacgggaatc atcggtattc
    15901 tgttggctaa cgaacaggta cgtctccaga ccagtgccag tagccagact tttgagaata
    15961 tgctgtaaca gatcattccc aagcaaatga ggataatacc gggagccagt cgggcgcttg
    16021 catcagaact gactaagacg tatatgccct gcagcaccgt gattgagccg agaaaaataa
    16081 cccaataaca ccacagccag tgctctttcg ttgaaaacgt attacgtgtt tgatggacga
    16141 ttgtggcaac aaggccaatt agacaggcac agattgcggt taacccgagc aatacgtgac
    16201 cggcgacata atgcggagtt atgtcggcac tacgtaacag cgtaatagac caaatgaaac
    16261 cgagcagggt gagtaacacg ggaacggcaa tgaggcaatt accgattaat gaagaataag
    16321 cctgtaccgg tgttccgtcg ctcttgctcc ccgctgcgtt tttaggaatg agcagaaagt
    16381 gaccggatga cgctgccacc gtcgatacac aggcggcaat cataccaacg ccgaaaataa
    16441 catggccggc gacaaactcg tctgccatca catcattacc tgctaacagt gcccaacccc
    16501 atatcatggt gataattgac cccgcatagc caataatggg gaacaatgta ttgtaaaacg
    16561 tattaacgcc gcgcgtgagc tgcgaaatga taataaatgc ggtagtgaat aatgccaggc
    16621 atatggctgc cagagaaatg agcacatgac ccgcgacgaa atactcgctt tgccctgaac
    16681 tttccagcac aaagccacct aacgcgatac aaatcaagcc cataattaaa gggattaatt
    16741 tgaaaagcca cccgatatat atgttcactt taaactcctc aggtatccct gctaatattg
    16801 aaatgtgcat taatgataat ttcagcctgt taatacaatg acgagatatc attaaagaca
    16861 aatgcatagc ataactatag cggggtttac tttgcagagc gctacttttc agcgttcaaa
    16921 aggccatgat aaataaatat ttatcctaag attaattctt attctgttgt tttttacaat
    16981 tatcttccag ggagggattc tttttcatta tttggtaaaa gaccggatgg tctcggtgct
    17041 taaaatcagc gagtatatac gcatcattgc tttatatcgt caaaaaatat gtctttcaga
    17101 catgtatcat ttattttgtt actaacaagt caaaaatatc acgggttatt tatgaaagta
    17161 tttcacaata tatttaagta catctcttca aaccatcaag ataagcattc agataaagtt
    17221 aataatcatc aacaccatgg caaggttgat aaaaaacatc gttcagagat agttgaaatt
    17281 gataagctgg ataaacattc gcaaatagat aacgattttg gcctgcatat catctatttc
    17341 ctgcaacatg gctgctggaa cgtaactgaa catagtcctc agatagagaa aatatggttt
    17401 tacaatagtg agccatctat agatattaaa gaatataata gtttcgccga caatactact
    17461 gacacgttta tctttacaat tataccagac aataaccatg tgataaaatt atcgtctccc
    17521 attaccgtta cgattgagcg taaggacggt tattatttta ttaatttctc cggggataaa
    17581 tcagacataa tttataaagt tgatgggttt tctatcaaag agagaaattt ctttactctt
    17641 cttagtggta atttcaaacc tgactggcgt tgggctgtct ctaaagagac gtttactaag
    17701 gaaaagtttg atagctatgt tgaatctgtt ttttcaaaaa tagactttta taaacagtgt
    17761 ggtgtgatta acccacaaaa tgccaacacg gcttattttg gcgatacaga tggaagagta
    17821 ggcgctgtac tttatgcatt gctcgtttca gggcatatag gcatcaggga aaaggggtgg
    17881 agtttattat gtgatttatt gaaacacgaa gacatggctt cattcgcata tgaaaataaa
    17941 aaattgaaaa aactcttcac tttattagat aagagagaca tgatcttgaa tgagttgcat
    18001 caacatgttt ttttaaaagg cgatgcgatt accccttgta tcttccttgg ggatcataca
    18061 ggcgatagat tcagtactat ttttggtgat aaatatatcc ttactttgct aaattctatg
    18121 cgaaatatgg agggtaataa agatagtcgt ataaataaaa atgtcgttgt tctggctgga
    18181 aatcatgaaa tcaattttaa tggtaactat agtgcacgac tagcaaatca taaactctct
    18241 tccggtgata catataactt aattaaaacg ctcgatgttt gtaattatga ctcagaaagg
    18301 catgttttaa ccagtcatca cggaataata agggatgaag aaaataaatg ttattgtctc
    18361 ggtgctttgc aggttccatt taatcagatg aaaaatccta ctgatcctga ggaattagca
    18421 ta
//
LOCUS       DAZKIF010000098        18394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZKIF010000098 DAZKIF010000000
VERSION     DAZKIF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.098"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            136..1356
                     /locus_tag="ACHOTW_003347"
     CDS             136..1356
                     /locus_tag="ACHOTW_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9845396.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1329..1958
                     /locus_tag="ACHOTW_003348"
     CDS             1329..1958
                     /locus_tag="ACHOTW_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP9845397.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1959..3119)
                     /gene="ybdL"
                     /locus_tag="ACHOTW_003349"
     CDS             complement(1959..3119)
                     /gene="ybdL"
                     /locus_tag="ACHOTW_003349"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP9845398.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3228..4316
                     /gene="hcxA"
                     /locus_tag="ACHOTW_003350"
     CDS             3228..4316
                     /gene="hcxA"
                     /locus_tag="ACHOTW_003350"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP9845399.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4326..4523)
                     /locus_tag="ACHOTW_003351"
     CDS             complement(4326..4523)
                     /locus_tag="ACHOTW_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9845400.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4705..6810)
                     /gene="cstA"
                     /locus_tag="ACHOTW_003352"
     CDS             complement(4705..6810)
                     /gene="cstA"
                     /locus_tag="ACHOTW_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP9845401.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(6991..7404)
                     /gene="entH"
                     /locus_tag="ACHOTW_003353"
     CDS             complement(6991..7404)
                     /gene="entH"
                     /locus_tag="ACHOTW_003353"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP9845402.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7407..8153)
                     /gene="entA"
                     /locus_tag="ACHOTW_003354"
     CDS             complement(7407..8153)
                     /gene="entA"
                     /locus_tag="ACHOTW_003354"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP9845403.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8153..9010)
                     /gene="entB"
                     /locus_tag="ACHOTW_003355"
     CDS             complement(8153..9010)
                     /gene="entB"
                     /locus_tag="ACHOTW_003355"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP9845404.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9024..10634)
                     /gene="entE"
                     /locus_tag="ACHOTW_003356"
     CDS             complement(9024..10634)
                     /gene="entE"
                     /locus_tag="ACHOTW_003356"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP9845405.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10644..11819)
                     /gene="entC"
                     /locus_tag="ACHOTW_003357"
     CDS             complement(10644..11819)
                     /gene="entC"
                     /locus_tag="ACHOTW_003357"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP9845406.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12008..12964
                     /gene="fepB"
                     /locus_tag="ACHOTW_003358"
     CDS             12008..12964
                     /gene="fepB"
                     /locus_tag="ACHOTW_003358"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9845407.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12968..14218)
                     /gene="entS"
                     /locus_tag="ACHOTW_003359"
     CDS             complement(12968..14218)
                     /gene="entS"
                     /locus_tag="ACHOTW_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP9845408.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            14329..15333
                     /gene="fepD"
                     /locus_tag="ACHOTW_003360"
     CDS             14329..15333
                     /gene="fepD"
                     /locus_tag="ACHOTW_003360"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP9845409.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15330..16322
                     /gene="fepG"
                     /locus_tag="ACHOTW_003361"
     CDS             15330..16322
                     /gene="fepG"
                     /locus_tag="ACHOTW_003361"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP9845410.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16319..17134
                     /gene="fepC"
                     /locus_tag="ACHOTW_003362"
     CDS             16319..17134
                     /gene="fepC"
                     /locus_tag="ACHOTW_003362"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9845411.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17131..18264)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOTW_003363"
     CDS             complement(17131..18264)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOTW_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP9845412.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4344 a         5209 c         4724 g         4117 t
ORIGIN      
        1 agacagctac tataactttc acctatttat tcacagcgcg aatgaaaaat aaaacactct
       61 gcttttcagt gtttacttta taattaacag tcttatctca ctgatcaccc tgtaacgttc
      121 agagagcgtc ttcagatgtc tatttataaa attcctcttc cgctcaatat tctggaagct
      181 gcacgagagc gtattacctg gacactcaac accctacccc gcgtttgtgt ctcattttct
      241 ggcggcaaag actctggttt aatgctgcat ctgacagccg aactcgcccg acaaatgggc
      301 aaaaaaatct gcgttttgtt tatcgactgg gaggcgcaat tctcttgcac cattaactat
      361 gttcagtccc tgcgcgagtt gtacaccgat gtcatcgaag agttttactg ggttgcgctc
      421 ccgcttacga cgcaaaattc cctttcacaa taccaacccg aatggcagtg ctgggaacct
      481 gatgtcgaat gggtacgtca gcccccgcaa gatgcgataa ccgatcctga ctttttctgc
      541 ttttaccagc caggcatgac cttcgaacaa tttgtacgtg agtttgccga atggttttca
      601 caaaaacgtc cggcggcgat gatgatcggc atccgtgcgg atgagtccta caaccgtttt
      661 gtcgccatcg ccagtttaaa taaacaacgt tttgccgacg ataaaccctg gactacggcc
      721 gcaccaggcg gtcatagctg gtacatttac cccatttacg actggaaagt ggctgatatc
      781 tggacctggt atgcaaatca tcaaagtctc tgcaacccac tgtataacct aatgtatcag
      841 gcaggcgttc ctctgcgtca tatgcgaatt tgcgaacctt ttggcccgga gcaacgacaa
      901 ggattatggc tctatcacgt tatcgaaccg gatcgctggg ctgctatgtg cgcacgagtc
      961 agcggggtaa aaagtggcgg catttacgcc ggacatgata atcatttcta tgggcatcgg
     1021 aaaatcctca agccagaaca tttagactgg caagaatatg cattattgct gctcaatagc
     1081 atgccggaaa aaacagctga gcattaccgc aataaaatcg ccatttattt gcactggtat
     1141 cagaaaaaag gcatcgaggt tccacaaacc cagcaagggg acattggcgc gaaagatatc
     1201 ccctcctggc ggcggatatg caaagtttta ctcaataacg attactggtg tcgggcatta
     1261 tcatttagtc ctacgaaagc gaagaactat cagcgttata acgaacggat aaaaggaaaa
     1321 cgtcaggaat gggggatact atgcaacaac gattaacgca ggatttaacg caatttctcg
     1381 ccagcttgcc ggaagatgat cgcatcaacg cgattaatga aatccgcatg gcgatccatc
     1441 aggttagccc ctttcgtgaa gagccagtgg attgcgttct gtgggttaaa aacagccaac
     1501 ttatgcctaa tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgaaaa
     1561 aatccattga aatagatggt tttacccaac ccattgtggt cacgcatacg gataaaaatg
     1621 ccctggaaat cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa
     1681 aattacgctt aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca
     1741 tcgccgcgac tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg
     1801 aaatcgtccg tgaactcagt cagttgggat gggacgataa taaaattggc aaagagctgg
     1861 gaatggacag cgacgaagtg ctgcgcctga agcaaattaa cggtctgcaa gagctgtttg
     1921 ccgaccgtca atattcccgc gcctggactg tcaaataact acagctggcg cagtcgttca
     1981 gctgctgcca gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg
     2041 aagggatcgg cgcaaaacac cgatagcgga atcgccgcca cgccgtgctc ccgcgtcagc
     2101 cactggcaaa actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag
     2161 tatgtgcctt cgcacggtaa aatctccagc cgactttcat ttaaggcatt taccagaata
     2221 tcgcgcttct ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc
     2281 atatccgcaa ttgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc
     2341 ttgcgaattt cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccggtc
     2401 atatgatagg tcttgccaaa cgatgacacc gccaccgccc gctcacgcag ctgcggatgc
     2461 gccagcacac tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta
     2521 atgacaaaaa tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc
     2581 cagacagttg cactgggatt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt
     2641 aacaatgcgg caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgc
     2701 ttcactattc ccccagaaag cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa
     2761 caaatcactt catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc
     2821 gcccctgccg ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc
     2881 gttttctgag caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt
     2941 gccccctggg caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca
     3001 ggaaagcctt gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg
     3061 aaaatagtgg tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt
     3121 tttattgtac ctgacgaagt tgcgtgttga aggcactata acacgatgct agtatttggc
     3181 aatcaagacg tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc
     3241 ctatccgcgt ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc
     3301 tgcacgattt tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcgtacgcg
     3361 ccattgctgc ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata
     3421 ttttgtttcg tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg
     3481 acgatcgcag cgtggtgatt ggcgtcggcg gcggcgcact actcgacacc gcgaaagccc
     3541 tcgcccgccg tctcggtctg ccatttgttg ccgctccaac gatcgccgcc acctgcgccg
     3601 cctggacacc gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt
     3661 tcgacgacgc caattttatg gtgctggtgg aaccggagat tatcctcaat gcgccgcaac
     3721 aatatctgct ggcggggatc ggtgatacgc tggcgaaatg gtatgaagcg gtggtgctgg
     3781 ctccgcaacc agaaacgttg ccgctaaccg tgcgactggg gatcaataat gctcaggcca
     3841 ttcgcgacgt cttgttaaac agtagcgaac aggcgcttgc cgatcagcaa aatcagcagt
     3901 taacgcaatc attttgcgat gtggtggatg ccattattgc tggtggtggg atggtcggtg
     3961 gtctgggcga tcgttttacg cgtgtagcgg cggcccatgc cgtacataac ggtctgactg
     4021 tcctgccaca aaccgagaag tttctccacg gcaccaaagt cgcctacgga attctggtgc
     4081 aaagcgcctt gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt
     4141 ttcatctgcc gactacgctg gcggagctgg aagtggatat caataatcag gcggagatcg
     4201 ataaagtgat tgcccacacc ttgcgcccgg tggagtccat tcattacctg ccagtcacgc
     4261 tgacaccaga tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg
     4321 caaatttagc agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg
     4381 aaaaactctt cgtaggtcat cggcgtttga tcgggatggt taacccgcat atgttcgaca
     4441 tagttgtcgt aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg
     4501 gcttttgcca gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg
     4561 gatgcggcgt gaacgcctta tccggcctac aaatgctatc aacgcgctcg gtcagccccc
     4621 tcgccccatc ggggagaggg ttagggtgag ggggaaccca cggcgccatg ccaacattcg
     4681 ccaacatccc ccctcactct gactttagtg cgcgcctttt gcctgcacca cgatctcctc
     4741 gacattttcc ggcattggct catacggcgt ttctttcgcc gttggcttcg gctctttcaa
     4801 tgccgcaagt gccgtcttaa tcgagaacag tgccagaacc accacgacca ccataaagaa
     4861 gatggttaac ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt
     4921 atactgcgat ggaatattac cgctgtcgat cattgcctgg aacttattag caatggccag
     4981 gaagccgact ttcgcatccg ggctaaacgc tttctgccag cctgcggtca gggtacaaat
     5041 cagcagccag gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa
     5101 caacaccacg gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa
     5161 cagcggccac agagtgttaa taccgcccag cggatcgacc acaccctgat gcaggaagta
     5221 gccccacgcc agcacgcaca gcgccgttgc cagcaggtta gcaggcagtg aatcggtccg
     5281 tttcaggccc ggagaaacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt
     5341 acctgcatcc accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca
     5401 gaacgccaca tccatcatgc cgcccagcgc gccatgcaga atgtaggcca tccccaccgc
     5461 caacgtcggc gcaccgcccg cacgggaaat gatcgactgt tctcccactt cgctggcaat
     5521 ctggttgagc gtatccggag taatcgcaaa gccccagcta ctcaccactt gcgcggcaga
     5581 agcgaccaca tccgccgtcc ctgccggagc cagcaccgcc atcgggctgt tcatggcgaa
     5641 gtacacgccc ggatcgatga tacaggcgga aaccagtgcc ataatcgcca cgaaggattc
     5701 cattaacatt ccaccgtagc cgataaaaca agcctgccct tcgttcgcca gcatcttcgg
     5761 cgtggtgccg gaagagatca gcgcatggaa gccagacacc gcgccacagg cgatggtgat
     5821 aaacaggaac gggaacaggt taccggtcca taccgggcca gtgccgtcaa caaatttggt
     5881 caacgcaggc atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccaacgat
     5941 cgtcccgatt ttcaggaagg tagagaggta gtcacgcgga gccagcagca accataccgg
     6001 cagcaccgcc gccacaaaac cgtaacccac cagcatccag gtcagctgca cgccggtaaa
     6061 gtcaaagtac ggtgcccagg tcggactttc tgccacccag ccgccagaaa taatggcgaa
     6121 aatcaggaat accagaccga tgaccgacac ttcaccaata cgccccggac gcagatagcg
     6181 caggtagatc cccatgaaca gcgccagcgg aatggtgaac gcaacggtat acgttcccca
     6241 cgggctatga gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat
     6301 gatcataaag caggccacca gcgcgatcac cccggcggtt ggccccatct cttctttgac
     6361 cagctcaccc agcgaacgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg
     6421 caccgcaccg gcgagaacca ccccggcgag tagccagatc atccctggca ggtaacccat
     6481 ttgcgccgcc agtaccggcc caaccaacgg acctgctccg gcaatggccg caaaatggtg
     6541 accgaacagc actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc
     6601 tggtgtcata cgcgtcgggt caaccgccag cacatttttg gcgatataca gaccataaaa
     6661 acggtaagcg atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg
     6721 ttccccacga tttaaagcaa tatatcccag agcaaatgct cccattacag agagcactgt
     6781 ccagacgagg tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga
     6841 tgttacattt tgtttctata acatctttgt acgatttaac aacacagcaa ccatgtaaat
     6901 cggagttgaa accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt
     6961 ttcctgcaat ctcatataat tctgtcgggt tcatcccaaa actgccgtac ccagccgaca
     7021 agtgcagcaa cgccgcccct gttcatcgaa aacgacgatt tcccagctct gattttgccg
     7081 cccaagatgc agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg
     7141 tgtggcgtta agttccgtgc ccaccacaca ctgcccgtcg cgggtcatca taaatccggc
     7201 catcgatccc agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg
     7261 ctgatgagta cggatatcaa ccggcatttc ggcttccagc acatcatcgc ccagacgggt
     7321 atacacaatt cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag
     7381 cgttaaatgg cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca
     7441 atatcctgta gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg
     7501 atctcttgtg gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag
     7561 ccgcgaatac gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggta
     7621 tcggtggaac ccggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg
     7681 acgctcaacg ccaggctttt cagcgccgct ttcgatgcgc cataagcact cataccaata
     7741 cgcggcgtgt gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga
     7801 aactggttca tggtttgctg gaacaggtta aacgcaccgc cgacattaac cgcaaaagtc
     7861 tgctgccagt cctccttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca
     7921 ttgaccagca cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc
     7981 tgcgcagcat cggcaacatc cattacttcg gtcgcaaagg gatattgctc ctgagcgaac
     8041 gcttgatcaa aacctgtaac tttcgctccc gcttcaacaa acgccagcgc cgtggcgtag
     8101 ccgatacctt tccccgcgcc ggttacccag acatttttac cgctgaaatc cattatttca
     8161 cctcgcggga gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc aacatgacaa
     8221 agtcgatatc gccatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat
     8281 ccagaccgta gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg
     8341 gcaggatcac ctcacgcagc gccgctttgc tggcggggat aggtgctggc agtaattctt
     8401 cggtcatcac cacccggcca gaacgtccgg ccacatattt cagcgacatc aaatgctcgt
     8461 cacggctgaa atcggccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg
     8521 cgtcggttgc ggtggtcata cagccaatgt gggcatatac cccggtaata atcagctggt
     8581 tacgtccact ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc
     8641 acttcaccag caccgtgtca tcggcatctg gcgtcagtcg atccaccacc ttttgctgtt
     8701 ccggcgaacg ggtcaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc
     8761 tctgctcttt cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc
     8821 gcagcgcagc aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc
     8881 tgacaaaata gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc
     8941 agtcaacttt attctgcgga atatcgtgag actccggcag tgcgtaagcc tgtaattttg
     9001 gaatagccat cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat
     9061 tgttttttat cgactttccc gaccgccgtc agcggaagtg aatccacaca ctccacgcga
     9121 tccggtaatt taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg
     9181 cgaagcggct cttttaccac cagataagca cagctttttt cgcccatcag ctcatcttcc
     9241 atactgacca gtgcggcgta gatcaccgcc ggatggcgca gtaacaggtt ttcgatctct
     9301 tcggcagcga tcttctcgcc gccacggttg atctgatctt tctcgcgccc ctgcacggtg
     9361 atgtaaccct ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca
     9421 aaggcgctgg cattgtgctg cgggcttttg taatagccac ggaaggtata cggcccgcgc
     9481 gtcatcagtc gtccgacttc cccttgcggc agtggatttc cttcggcatc ggcaacccat
     9541 acttcgtcat ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca
     9601 tcaagacggg tatagttcac cagcccttcc gccataccaa acacctgctg caactggcaa
     9661 ccaatctcag cgggaatacg cgccgcaagc gtggcagaaa gacgtgcgcc accgacctgt
     9721 aacagtttca gcgaggcaag ctgcgcccgg ctttcgcctt cggtcagcgc ctgcaaccac
     9781 aggctgactg ccggcggcac cagcgcggta acgttaatct gatgtttttc aatcaatggg
     9841 aagcaaagcg tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg
     9901 cccagcgatc ccggcgaact catggcgtag ttatgagccg ccgggatcgc gcacaggtag
     9961 cgtgtctgtt gtgtgaactg acaaatctcg acgctacgac gcacgctgta gtagtagtcg
    10021 ttatgagtgc gcgggatcag cttcggtgta ccggtggtgc cgccggaaag ctggaaatag
    10081 accacttcat cagcaggtga cggcgtagca gtaaaaccgt ctgccggatg gttaatcgca
    10141 tcctgcaagt tatgctcacc gctgtcgttg agtagctgca ccacgcgaat ggaagaatgc
    10201 tctgcaacaa atgtgttgag gaaatcatcc ccgctaaaca gcgcatgttg gcgatcggca
    10261 atcagcaatg cgggttcaat ctggctggca taggcgttca gttcactacg ctgatggctg
    10321 aacaacgcca gcaccggcgc aacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt
    10381 tcagcgacgt tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgt
    10441 aaactacacg cgaggttatc cgccgcctga ttcagctccc ggtaactcaa ctgtcgctcg
    10501 ccgtcgataa ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc agtcagtggc
    10561 aaatcctgcc agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg
    10621 aatggaatgc tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt
    10681 ttgacgcctg tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg
    10741 gcaaacagac gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg
    10801 ccgttacctt cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaacggc
    10861 tccagttcag caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc
    10921 ggatgcagca gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag
    10981 ggagttgcga gatgccacag cgtcggcgtg gtaatcaatt gtggagagga aggaacgtgt
    11041 aactcactac tgcgttcgcg cagtacctct ttcatcgcct gagtcaccag ttcatgttca
    11101 tggcgatctt tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc
    11161 ggctgacgac gtgcggaacc ggctaacgga atggagctaa aacgctcacc gtctttacgt
    11221 agcagcagtt ccgggctggc ccccagcaga acgccaccat ctgccagcgg gacatggaag
    11281 ttgtaactaa ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg
    11341 gcgtcagtgg tgatatcaat caaccgtgac aacaccactt tgtcgacctg cggcgtggcg
    11401 gtaagtgcgg cggcgcgggc aaccatctgt tcaaacgtgg tttgctccgg aattgcctgg
    11461 cgttccacca cgttcagcga ctggctgcgg gtgaaacggc gtgcggaagc ttgtttttcc
    11521 tgacgggaga acgactgcca ggattcagga atatacagcg acgaaggctg acgtggatcg
    11581 aaaggaatcg ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac
    11641 agcgcggcga gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca
    11701 tcgaagcggg cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag
    11761 cgattgggcg caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata
    11821 tcatcctcca caaaatgata aaggctttaa taatgattat catttatatt ttcggtcgct
    11881 aacctaaaag caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg
    11941 ttgcatctgg ttgtggttat aatgaaaatg agaagcatta acttcattaa taacaggagg
    12001 ctgatttgtg agactcgccc cgctctaccg caatgccctt ctattaacag gacttttgtt
    12061 ttcaggaata gccgcagttc aggccgccga ctggccgcgt cagattactg acagccgtgg
    12121 cactcatacc ctggaaagcc agccgcagcg tattgtttcc accagcgtca ccctgaccgg
    12181 ctcactgctg gcgattgatg ctccggtgat cgccagcggc gcgaccacgc cgaataaccg
    12241 cgtcgcggat gaccagggct ttttacgcca gtggagcaag gtggcgaaag aacgcaaact
    12301 gcaacggctc tatatcggcg aaccgagcac cgaagccgtt gccgcgcaaa tgccggatct
    12361 gattttaatt agcgcaaccg gcggggattc ggcgctggcg ctgtatgatc agctttccac
    12421 catcgccccg acattaatca tcaattacga cgacaaaagc tggcaggcgc tgttaacgca
    12481 acttggcgaa attaccgggc atgagaaaca agcggcagag cggattgcgc agtttgataa
    12541 gcaactggcg gcggcgaaag agcaaatcaa attaccgccg cagccggtca ctgccattgt
    12601 ctatactgcc gctgcacaca gtgccaatct ctggacgccg gaatcagcac aagggcagat
    12661 gctggaacaa ctcggcttta cgctggcgaa gttacccgca gggttaaatg ccagccaaag
    12721 ccagggtaaa cgccatgaca tcattcagct tggtggggaa aatctggctg cagggttaaa
    12781 tggcgagtca ctattcctgt tcgccggtga tcagaaagat gccgatgcta tttatgctaa
    12841 tccgctgctc gcgcacctgc ctgcagtaca aaacaagcag gtttatgcgc tgggaaccga
    12901 gacgttccgt ctggattact acagcgccat gcaagtgctg gaacgactta aggcgctgtt
    12961 ttaagcatta accgtcagac gctgtcacct gcggcggcgt ctggcgaaaa cgtcgcaact
    13021 ccaccagcac cagcaataac aacacgccga taatcaataa accaaaaccg ctcgcgcttg
    13081 cggaagcaac cggtgtcatc atcgctccca aaccacccag cagcgccgcg cctatggcat
    13141 cgcccgtcac gttttgtgcc gtccacaaac cgttaatccg ccctaacatc gcttccgggg
    13201 tttgcgtttg cagcattgtg tattgcagca acgagctgac cgcactcaac cagccgaaca
    13261 gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga ccaatggcga
    13321 ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcgcta tgcgccagct
    13381 tcccgctggt taacgcgcca atagccgcgc cgagcgggat cgccgcatag agaaaaccaa
    13441 tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacagtacc cgcaccgcac
    13501 tcgccatcgt caataaacca cccagcagcg caatccctcc caccagcggg ctggcgagta
    13561 gaaaacgaaa tcctgccagt aatgatttca acggatgctc acgcggctgc ggtggcggtg
    13621 gcagtgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc gccgccagcc
    13681 cgtagttcca ggccacaccg ccagtcgcca gcagtaaacc gccaatcatg ggcgaaatca
    13741 ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag ttttcacgcc
    13801 ctaccagtgc tggtgtcgcc gccaatagcg ccgtaacgcc aagcgatgcg aaaaaaccat
    13861 cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagc gcgttcaggc
    13921 acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact tttttgcgct
    13981 catagcgatc cgccagcaca ccgccgacca tcaggccaac aaacatcgcg ccgccggtca
    14041 gcgtcaccga aagccccacc tgccaggtgg aatgtgtcat catctggatc tgcaccggca
    14101 ccgcgacgcc gagcaaaccc agagacacaa ttgagatgaa acgagcgagg aatactgcgc
    14161 gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt ttattcatta
    14221 caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg ctaacatatc
    14281 caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat gtctggttct
    14341 gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat tatcgcgacg
    14401 gcattaagcc tgctcattgg ggcaaaatca ctccccgctt ccgtagtgct ggaggccttc
    14461 tccggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcggct gccgcgtacc
    14521 cttgccggtt tactggcagg cggcgcgctt ggccttgccg gggcgttaat gcaaaccctc
    14581 acccgaaacc cacttgccga ccccggcttg cttggcgtga acgccggagc cagctttgcc
    14641 attgtgctgg gtgcggcgct gtttggttac tcttccgcac aggaacaact ggcgatggcc
    14701 ttcgccgggg cgctggtggc ctcgttgatt gttgccttta ccggcagcca gggcggtggg
    14761 cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt gctggaaggg
    14821 ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg tttctggcaa
    14881 gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat cccagtgctg
    14941 atcgccggag caactgcgct attactgagt cgcgcgctga acagtttaag ccttggcagc
    15001 gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttgattgg tctgctggcg
    15061 attaccgtgc tttgtggtag tgcgacggcg gtagttggcc ctattgcctt tattggcctg
    15121 atgatgccgc atatggcgcg ttggctggtg ggtgccgatc atcgctggtc gctgcccgtc
    15181 acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg ggtgattgtg
    15241 cccggtgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt gctgatcttc
    15301 ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga ttactcatca
    15361 cctgtttgct gctgatttcc gcctgtgtgg ttgcaggtat ctggggatta cgcagcggtg
    15421 ccgtcacgct ggaaatctcg caggtattcg ccgcgctgat gggcgatgcg ccgcgcagta
    15481 tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg ttgattggcg
    15541 cggcactggg cgtcagcggt gcgatttttc agtcgctgat gcgtaaccca ctcggcagcc
    15601 ctgacgtaat gggctttaac accggggcgt ggagcggcgt gttggtggcg atggtgctgt
    15661 ttggtcagga tctgacggct atcgcgctgg cagcaatggt gggcggcatt atcacttcgc
    15721 tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg attattatcg
    15781 gtatcggcgt tcgcgccatg ctggtggcct ttaatacctg gctgttgctg aaagcgtctt
    15841 tagaaacggc gctaacagca ggtttgtgga atgccggatc gctcaacggc ctgacgtggg
    15901 caaaaacctc gccttccgca cccatcatta tattgatgct aattgccgcc gccttactgg
    15961 tacgccggat gcgcttgctg gaaatgggcg atgataccgc gtgtgcgctg ggcgtcagcg
    16021 tcgaacgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct gcggcaacag
    16081 cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc cgacgcatta
    16141 gcggcaccgc tcgctggggg ctaacccagg cggcgctatg cggggcgctg ttactgctgg
    16201 cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt ggcgtcgtta
    16261 ccgtcagcct cggcggtatt taccttatcg ttttgttaat tcaggagtct cgcaaaaaat
    16321 gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg gcaaatatac
    16381 cgttgcggaa aatctgactg tagaaatacc tgatggtcac ttcacggcaa ttatcgggcc
    16441 aaatggctgc ggtaaatcca cgttgctgcg taccttaagc cgcctgatga cgcctgctca
    16501 tgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag aagttgcccg
    16561 gcggattggt ctgttggcgc aaaatgccac cacgctggga gatatcaccg tgcaggagct
    16621 ggtggcgcgt gggcgttatc cacatcaacc gctgtttacc cgttggcgca aagaagatga
    16681 agaggcggta acgaaagcga tgcaggccac gggaataact catcttgcag atcaaagcgt
    16741 ggataccctt tccggtgggc aacgccagcg agcgtggatc gcgatggtgc tggcccagga
    16801 aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc atcagattga
    16861 tttgctggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg cggcggtgct
    16921 gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc gagaagggaa
    16981 aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg agcgcattta
    17041 tggtctccgc tgcatgatca ttgacgatcc ggtagccgga acgccgctcg tggtgccgct
    17101 cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca tcatggcatc
    17161 ctgttttctg gatgccatcg catagcgcaa taacacgcta ccacaagcca ccatcccgcc
    17221 gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt tcactggcaa
    17281 tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgatat ttgcttttgt
    17341 taactgctca acaagatact gacgattacg taattcaccg ttcagttccg caacgtcagt
    17401 gaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag aaatagagaa
    17461 atcggggtca tctttaacgg cctgaccatt actgtaaacg ggctttttaa ttcctgccgc
    17521 gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt gatttttcat
    17581 tttaatgcgg tcctgagcca gtttttcttt ttcaaactgg gttttgatct ccagtttatt
    17641 acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga tatacccgct
    17701 caaaacggtc tgcgcctctt cactggttgg cgcggtaaaa cttagcgtcc aggaggtata
    17761 cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca ttttttcgct
    17821 caatgcgaca attgcgcgat gcaaatccag ttcgtcgatt ttcgcctctt ttaattggtc
    17881 catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaaccgact gaaacttctt
    17941 gataaacagg ttaaatgctt ctgtacgatc aattttgata tccagatcca gcacacgaag
    18001 cttagtgaat gttttctcca actcttgcca ctgaacaggt tctggaggcg tgacaaccgc
    18061 cgcgctggtc catttttgcg gcaggatgaa agagatcagc aagcctgcac aggcaaacgc
    18121 aaaaacgacc gccatgaccg tttttttggc ccgccataaa actgagatta gattaagtaa
    18181 atcaatttca ttattactgg gcgacgccag aggataatcg ggaaaatgag cgtcacttcc
    18241 ctgtttaata ttcagtgatg acatggggta taacctgaaa atgagtccaa tagggggaaa
    18301 tctccacggc gctcacttta ccagttggtg agaattatcc gaagctgaag tttgtaaatt
    18361 cattccactg aatgcaacat tcactctatt taag
//
LOCUS       DAZKIF010000099        17649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZKIF010000099 DAZKIF010000000
VERSION     DAZKIF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.099"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..162)
                     /locus_tag="ACHOTW_003364"
     CDS             complement(<1..162)
                     /locus_tag="ACHOTW_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845413.1"
                     /translation="MRALCAVNGSHPENLRFWLKACPVVLRERFHQQCKCRFNWSTQQ
                     LCENMGLRRF"
     gene            complement(183..464)
                     /locus_tag="ACHOTW_003365"
     CDS             complement(183..464)
                     /locus_tag="ACHOTW_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845414.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(1003..1089)
                     /locus_tag="ACHOTW_003366"
     tRNA            complement(1003..1089)
                     /locus_tag="ACHOTW_003366"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1053..1055),aa:Leu,seq:taa)
     gene            complement(1102..1175)
                     /locus_tag="ACHOTW_003367"
     tRNA            complement(1102..1175)
                     /locus_tag="ACHOTW_003367"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1141..1143),aa:Cys,seq:gca)
     gene            complement(1229..1304)
                     /locus_tag="ACHOTW_003368"
     tRNA            complement(1229..1304)
                     /locus_tag="ACHOTW_003368"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1269..1271),aa:Gly,seq:gcc)
     gene            complement(1456..2004)
                     /gene="pgsA"
                     /locus_tag="ACHOTW_003369"
     CDS             complement(1456..2004)
                     /gene="pgsA"
                     /locus_tag="ACHOTW_003369"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9845415.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(2061..3893)
                     /gene="uvrC"
                     /locus_tag="ACHOTW_003370"
     CDS             complement(2061..3893)
                     /gene="uvrC"
                     /locus_tag="ACHOTW_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9845416.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(3890..4546)
                     /gene="uvrY"
                     /locus_tag="ACHOTW_003371"
     CDS             complement(3890..4546)
                     /gene="uvrY"
                     /locus_tag="ACHOTW_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP9845417.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4688..4804
                     /locus_tag="ACHOTW_003372"
     CDS             4688..4804
                     /locus_tag="ACHOTW_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9845418.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            4842..5018
                     /gene="yecU"
                     /locus_tag="ACHOTW_003373"
     CDS             4842..5018
                     /gene="yecU"
                     /locus_tag="ACHOTW_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP9845419.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            5005..5229
                     /gene="yecF"
                     /locus_tag="ACHOTW_003374"
     CDS             5005..5229
                     /gene="yecF"
                     /locus_tag="ACHOTW_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP9845420.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(5297..6019)
                     /gene="sdiA"
                     /locus_tag="ACHOTW_003375"
     CDS             complement(5297..6019)
                     /gene="sdiA"
                     /locus_tag="ACHOTW_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP9845421.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(6249..7001)
                     /gene="tcyN"
                     /locus_tag="ACHOTW_003376"
     CDS             complement(6249..7001)
                     /gene="tcyN"
                     /locus_tag="ACHOTW_003376"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP9845422.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(6998..7666)
                     /gene="tcyL"
                     /locus_tag="ACHOTW_003377"
     CDS             complement(6998..7666)
                     /gene="tcyL"
                     /locus_tag="ACHOTW_003377"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP9845423.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(7681..8667)
                     /gene="dcyD"
                     /locus_tag="ACHOTW_003378"
     CDS             complement(7681..8667)
                     /gene="dcyD"
                     /locus_tag="ACHOTW_003378"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP9845424.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(8772..9572)
                     /gene="tcyJ"
                     /locus_tag="ACHOTW_003379"
     CDS             complement(8772..9572)
                     /gene="tcyJ"
                     /locus_tag="ACHOTW_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9845425.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(9660..10211)
                     /gene="fliZ"
                     /locus_tag="ACHOTW_003380"
     CDS             complement(9660..10211)
                     /gene="fliZ"
                     /locus_tag="ACHOTW_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP9845426.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(10257..10976)
                     /gene="fliA"
                     /locus_tag="ACHOTW_003381"
     CDS             complement(10257..10976)
                     /gene="fliA"
                     /locus_tag="ACHOTW_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP9845427.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(11296..13053)
                     /locus_tag="ACHOTW_003382"
     CDS             complement(11296..13053)
                     /locus_tag="ACHOTW_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFP9845428.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            13319..14716
                     /gene="fliD"
                     /locus_tag="ACHOTW_003383"
     CDS             13319..14716
                     /gene="fliD"
                     /locus_tag="ACHOTW_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP9845429.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            14741..15151
                     /gene="fliS"
                     /locus_tag="ACHOTW_003384"
     CDS             14741..15151
                     /gene="fliS"
                     /locus_tag="ACHOTW_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP9845430.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            15151..15516
                     /gene="fliT"
                     /locus_tag="ACHOTW_003385"
     CDS             15151..15516
                     /gene="fliT"
                     /locus_tag="ACHOTW_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP9845431.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            15594..17081
                     /gene="amyA"
                     /locus_tag="ACHOTW_003386"
     CDS             15594..17081
                     /gene="amyA"
                     /locus_tag="ACHOTW_003386"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9845432.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(17115..17528)
                     /gene="yedD"
                     /locus_tag="ACHOTW_003387"
     CDS             complement(17115..17528)
                     /gene="yedD"
                     /locus_tag="ACHOTW_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP9845433.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT         4523 a         4539 c         4175 g         4412 t
ORIGIN      
        1 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atctacactt
       61 acattgttga tgaaatcgct cacgaaggac caccggacag gctttcagcc agaagcgcag
      121 attttcggga tgtgaaccgt taacggcaca taatgctcgc acaacaaacc tgttaaaaga
      181 gattactcag cattctcctg cttccattgc cggatcattt catcggtaac atcaccctca
      241 taacatacca cgtcataccc tccattacgg ctatatgcac tgcgtccacc acatctacta
      301 ccattccttg catgattata aggacacgca caattgcctg gataagattc aatggattct
      361 ttaattattt cttttttgat ctgagcatca gactttcctg ttgctgcata tccactaaaa
      421 gatactaaac aaaggcagat tgccattaat aaaccacatc gcataccgat acactcaatt
      481 actaaccaat catagcccac atcataaaat cgagatgagg agcacaatac ctttaacaag
      541 aaggtgtcat aacgataata tgtcgtgcaa ttcatcaaaa aaaagatcta tatcaaagag
      601 atagaactct tatcagtgct tcagacatcg gcattcgagg cagcagaaaa acagtaaatc
      661 tgacgaattt gccttttccc aagatgttgt cagattaaaa agatagatta gcgcaggtac
      721 ctgttggtgg attgcaggag gaaaaataaa gtcgagtgga aaaatcccag aaaaattttt
      781 gtttaacaga ggtcctggtt cactatgcga aatacggagg agctgcagaa gaattctgct
      841 tcatccttag agtttaaaca gcacgtgcac taccattgtt tctgcatatc gccacaccat
      901 cgccactcta ccgccattgc acaaaataca aatacgaaaa aaccacccga aggtggtttc
      961 acgacactgc ttattgcttt gattttattc ttagctttcc catggtaccc ggagcgggac
     1021 ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc cttgtgtcta ccgattccac
     1081 catccgggct cgggaagaaa gtggaggcgc gttccggagt cgaaccggac tagacggatt
     1141 tgcaatccgc tacataaccg ctttgttaac gcgccaaatt cttcaggcct ttcagccaga
     1201 catccgctga cgccgatgtc ttttaaactg gagcgggaaa cgagactcga actcgcgacc
     1261 ccgaccttgg caaggtcgtg ctctaccaac tgagctattc ccgcattcat caagcaatca
     1321 gttaatcact tgattttatt atcgtctggc aatcagtgcc gccgttcgat gcgttgcatt
     1381 ctacttacct ggcgcgatga gtcaacgata tttttcacca cttttgatcg tttgctgaaa
     1441 attgcgccga aacgatcact gatcaagcaa atctgcacgc gcagcgctca aatattgcaa
     1501 cattgaccac agagtcagta ccgcagccac aaagaaaagt gcaataccgg cgtactcaac
     1561 ccaaatgttc ggacgccaca gcagccatgc caacgccacc atctgggcag tggttttcac
     1621 tttcccaatc caggagacag ccacgctact gcgtttaccc aactccgcca tccattcgcg
     1681 tagcgcagaa ataataattt cacgggcgat catcgttgcc gccggtaagg tcacccacca
     1741 gctgtggtaa tgctcggtta ccagcaccat ggcgatagcc acgagaactt tatctgctac
     1801 agggtcaagg aaagcaccaa accgggtact ctggttccag cggcgtgcca gaaaaccatc
     1861 gaaccagtca gtcaccgccg cgacgcagaa aatgagcgcg gcggcaaacg gcgaccaagt
     1921 gacaggcaga taaaagacca atacaaagaa tgggataagg atgacacgga acagtgtaag
     1981 caacgtaggg atattaaatt gcataatgac gggtaactat ctgttgtcag taagattacc
     2041 cctatgttgc tacagagaca tcaatgtttc aacgaccaga agatcttttc tgccagacct
     2101 tgcgaaatac ccggcacttt tgcaatttcc tcgacgctgg cgttacgtaa accttgcaaa
     2161 ccgcccatat atttcaacaa catttgccgg cgttttggcc cgacgccttc aatggtttcc
     2221 agggaactgg tatttttgac cttcgcccgt tttttacggt gcccgccaat cgcgtgatcg
     2281 tgtgattcat cgcgaatatg ctggataaca tgcagcgcgg gagaatctgg cggcaaactg
     2341 aatccctcac cttccggctc aaagaacagc gtttccagcc cagccttacg atctgctcct
     2401 ttggcaacac caagtagcag cggatgattt ttatcccatg agacatccag ttcggcgaag
     2461 acatttttcg cctgcgcaag ctggcctttg ccgccgtcga taagtatcac atccgggatc
     2521 ttactgtcgt cgatggcttt accataacgc cgacgcagca cctgattcat cgccgcataa
     2581 tcatcgcccg gcgtgatgcc agtaatgtta tagcgccgat actccgcacg cagcgggccg
     2641 ttagcatcaa acaccacaca ggaagcgacg gtttgttcac ccatggtatg gctgatgtca
     2701 aagcactcca tccgcttcac ttccggcaat ttcaacacgc tggcaagtgc tgtaagccgc
     2761 tggtgaacgg tagattgctg cgaaagtttg ctggttaagg ccgtcgccgc attggtgcgc
     2821 gcgagtttca ggtaacgcgc cctatcgcca cgaggtttgg tttgaacatt aatcttgcgt
     2881 cccgccagtt ctgaaaggga atcggcgagc agcgttttat cgctaagatt aaaatcgagc
     2941 aggatctcac ccggtaaggt gcgcatctgg ctgccttgta aatagaactg gcctacgaag
     3001 gtttctacca cctcgctcag ttccgtaccg ccaggcactt tcgggaaata gctgcggctg
     3061 ccgagcactt tgccctgacg aatgaacaat acgtggacac aagccatacc cgcatcgaac
     3121 gccacaccaa taacgtcgag gtcgtcgccg gtattggaaa cgaattgttt ttcggtgacg
     3181 cgtcgcaccg cctgaatttg gtcgcgaata cgtgccgctt cttcaaactc cagattctga
     3241 ctggcagttt ccatacggct aatgagttgc gtaagcacct gatcatcttt gccagacaaa
     3301 aacaggcgca catactcgac ctgctgagcg tattcttctt cactcaccag tccttcaacg
     3361 cacggtccca gacagcgccc tatctgatat tgcagacacg gacgcgagcg attgcgataa
     3421 acactatttt cgcactggcg aatggggaaa atcttttgca gtagcgccag tgtttcacgt
     3481 acggcatagc cattcgggaa cgggccgaaa tattcacctt tggcatgctt tgcaccacga
     3541 tgcatcgcca gacgcgggtg ggtatcgcca ctcaggaaga taaaaggata tgatttatca
     3601 tcgcgtagca aaacgttgta acgcggctga tagagtttga tgtagttgtg ttccagcaac
     3661 agcgcttcgg tttctgtatg agtaaccgtt acatcaattt gctggatctg ggcgaccagc
     3721 gcttcggttt tgcgcgaagc gaggttgcta cggaaatagc tggaaagccg ttttttcagg
     3781 tctttcgctt tgccgacata gataaccgta ccaccagcat cgtacatgcg ataaacgcca
     3841 ggctggctgg ttacggtttt taaaaacgct tttgcgtcaa actgatcact cactgacttg
     3901 ataatgtctc cgcattacac agaccatggc gaattgccag gtgagtcagc tcaacatcgc
     3961 catgaatgtt tagtttactg aacatacgat agcggtagct gttcaccgtt ttcggactga
     4021 gattgagctg ttctgagatc tcattgacct tctggccctt cgtgatcatc agcataatct
     4081 gcaattcacg ttcagacaaa ctggcaaatg ggctttctgt tttttctggt tcgatctggc
     4141 ttaatgccat ttgttgagcg atgtcagaag caatgtaacg ctgcccagaa tagacagaac
     4201 ggatcgcact cacgacttcc tgcggagccg cgcctttgct gaggtagccc gcagcaccgg
     4261 cctgcatgac tttcgctggt aaagggtttt ctgtatggac ggtaagcatg atgattttta
     4321 catcagctgt ggaacgcgcg attttacgcg tcgcctcaag accgccaatg cccggcatac
     4381 tcatgtccat tagcaccacg tcaacggcat ttgcccggca ccacttaacg gcgtcttcac
     4441 cgcacgatgc ctcaccgacg acttttatac cctttatatc ttccagaatg cgtcgtatcc
     4501 ctgcgcgcac cagttcgtgg tcatcaacaa gtagaacgtt gatcaaagga atatctccag
     4561 aaatagggat aacgctactg atagttagtc attcgtatat tagcggtttt tattgcaact
     4621 ttgaaacgtt aaaaatggtg ggtattcgat ttttctctcg tttttgtcaa ttcgattgtc
     4681 cacagttatg gtcacgcccg tcaccaacgg cttaccggat cgtttcacag taagccctgc
     4741 gacgaaatgt gacaaaaatt acctttattc agcaaaaatg aaaatcagcc cgctgaatat
     4801 gtaacattaa ttaacccgaa caatacgggc aaaaaacatt catgataaaa atatttattg
     4861 gtcattatat taacgcattt aatagcaccg ttgacggcac gctcaaaaaa caaccactgc
     4921 tatttttagc aaagcttatg gtatactccg ccgccttaac atttttcacc gcaaattttc
     4981 attgcaacat gacgaggaaa ataaatgagt acgcctgatt tttctactgc cgagaataat
     5041 caagaactgg caaatgaagt ctcctgcctg aaagcgatgc tgacgctgat gctgcaggcg
     5101 atgggacaag ctgacgcggg ccgcgtgatg ttaaagatgg aacaacagct tgcgctgatc
     5161 gaagacgaaa cccaggctgc agtattttcc aaaacggtta agcaaattaa acaagcctac
     5221 cgtcagtaat ataaaaccgg ctgatagcgt gcctttcagc cggtttttgc atctggcacg
     5281 caggacagaa aagagatcaa attaagccag tagcggccgc gtaacaggca acctgggtct
     5341 tatttggtgc attaattttt ttctgcatgt ttttctggtg gaaattgacc gtattctcag
     5401 agattgacaa aatcatcgct atctctgctg atgtcttccc ttccgccgtc cacctcagaa
     5461 tttctttttc gcgcttgctg aaattcatct ctggcgtcat cactatttca tcatttaaac
     5521 gcatcagagc catcagactt tcgcgcacca gtaactgcat ttttaattgc agttcatcac
     5581 taagaatggg tatttcgcgc gcgctgcaac gggaaaagga caaaaagccc agcgcccggt
     5641 ttggcagcat taaatactga gtgacaccgc ggcgtaaacc atgtgcgcgc gcggcttccc
     5701 ataacggctg tgcttcgttg aataagtcat cattccacat tagatggccc tgactaaagt
     5761 tttcagggtt cagcaccgga tcaattgcga gaaagttttt tgcctgataa taactaaccc
     5821 acgcctcagg gtaattggtg tagaaagcca ctttaggtcg agtgaatggt accgggtggc
     5881 ggacacataa cgaatagtaa tcgtactcca gctgctgagc ctgaagctca atttcatggt
     5941 agacctcttc tgcggtctcc atcctctgaa aacgcaacag catcgtgcga cgccagctga
     6001 aaaaatccgt atcctgcata gtaaaccgca acgcccctga gagtgagtat catttataat
     6061 gataattcaa actatcacat aaataagatt tatatataat ttatattatt caggcaatga
     6121 attacttttg caagccatcg cattctctta tgttattaat gagttatgct gattttttaa
     6181 gcagttttat caggcttgaa atggcgttcc gccccgactg tcgaatcgtc ggggctgatt
     6241 ttttcttatt attgcagcag aaacttctcg aggaactggc gggtgcgagg ctgctcggga
     6301 tcggcaaata acgcttttgc ggccccctgc tcgactatcc gcccctggtc cataaaaatc
     6361 gcccggtccg caacatcccg ggcaaagctc atttcgtgtg tcacaatcac catcgtgcgc
     6421 ttttcctgcg ccagctgacg gatggtgttc aggacttcgc ccaccagctc tggatccagc
     6481 gctgacgttg gctcgtcaaa caaaatcacc tcaggacgca ttgccagcgc acgcgcaatc
     6541 gcaacacgct gctgttgacc gccagacaaa cgacgtggat agctggtttc tttaccggcc
     6601 agcccaactt tcgccaacag cttgcgagcg cgcgccgtgg cctcttcttt cggttcgcct
     6661 ttgacgatca ccggcccttc aataatgttc tccagcaccg tacgatgcgg aaacaaatta
     6721 aagttctgga agacaaaccc gacgtgctga cgcaactggc gaatcagcga tttttgctga
     6781 cttaatgaac gtgcagtatc aatagtgata tcgccaacgg tgatcgttcc cgcttccggt
     6841 tgttccagca gatttatgct acgtagcaat gtggttttgc cggaaccact cggaccgata
     6901 attgccacca cttcgccagg ctttacctcg aggtcgatac cgtgcagcac cgtctgaccg
     6961 tggaattttt tcactaggtt cttaacttca atggcactca tttgggttct ctctcctggc
     7021 gattaagttg attctcaaaa tggttctgca acgtcgataa cacagtcgcc atgatccagt
     7081 agatgagcga agccgccaga tacatggtga atacctccag cgtacgcgag gtaatcaact
     7141 gcgcctgacg gaacagctcc ggcacctgga ttgtcgcggc cagcgacgta tcttttacca
     7201 gactgataaa gctgttcgag agcggcggca acgccacccg cgccgcctgt ggcaaaatcg
     7261 cgcgacgcat agtctgccat ggcgtcatac caatactggc agctgcttcc cactgacctt
     7321 tatcaataga agaaatggcg gcgcgaagcg tttcggcagt ataggcggca gtgttgagcg
     7381 acagaccaat cattgctgac ggaatgggat ctaattcgat accaaactgc ggcaggccgt
     7441 aatagatcat aaacagttgt gcgatgagcg gcgtaccacg aaaaatagag atataaaaac
     7501 gcgccagcca gcgcacgggc cagattggcg acaggcgcat cagcgcgaga ataaacccta
     7561 gcagtaaacc aaaaaacatg ccgccgatac tgagttgcag cgtataccca gcccctttca
     7621 gcaggaacgg caaggaatca ataaccagtt gtatactttc ttgcatgagc gttcccggag
     7681 ctaaacgtgg ggatgatagg cgaacagcgc aggcgcgccg ccggtatgaa taaacagaat
     7741 cggcccttca tctttgaagc gtttctgact gataccgtca atcagacccg ccatcgcttt
     7801 tccggtatac acaggatcaa gcagaatgcc ttccagccgc gccagcaatt tcactgcttc
     7861 catgccttcg tcgttcggca cgccgtagcc aggtgcaaaa tagtcatccc agagtaaaat
     7921 ttccactgat gcggtcagct ccagttcttt cgcaatcgcc tgttgtaggt taaccacttt
     7981 cggcaattga tcggcaacgg aacgcgacac ggtcacgcca atcagttcgc tttcaggcat
     8041 caggtgttcc agcccaacag ccagtccggc gtgagttccg gcactgcccg atgcgactac
     8101 caccgatgaa atattaaccg ccccttcaca ctgttgcgcg atttccagcg cactctccac
     8161 ataacctagc gcgccaagag cattagaacc gccaaccgga atgacatacg ggcgaaagcc
     8221 ttgtgcttcg actcgcgtcg ccagctcttc cagttgggca ttgggatcgg tcagtgcgtc
     8281 gcacatttca atctgggtat tgaacagatc cagcaacaag cgattgccgt tggttaaata
     8341 gttttctgcg gttgtgccaa taggattttc cagcagcgcc acgcagtgca gaccgagttt
     8401 ggcagcgact gcggcagtct gccgcacatg gttagactgg atcgccccgg cagtaatcag
     8461 cgtatcggca ccttcacgca gagcatctgc cgcgagaaat tctagcttac gtaatttatt
     8521 gccgcccatt gccatggggg tgacgtcatc ccgtttgatg aaaatttccc gtcctagata
     8581 atcagaaaag cgcggcagat attcgagcgg cgttggcgcg ccgataaact ccagccgtgg
     8641 aaaacgggtt aaattatgca gtggcataac agcctccgat gtgtgttgtt gtgattttct
     8701 cattatgcac gctgaaaacg cgtaaataaa aaaggcgcta gtgaaagcgc ccttttttgt
     8761 cattatgctg attatttggt cacatcagca ccaaaccatt tttcggaaag ggcttgcaaa
     8821 gtgccgtctt tttgcatttc cgcaattgca tcattcactg ctttcagcag gtcttcattt
     8881 cctttacgca gcgccacgcc agactcctga cgggagaatg cttcaccggt tactgccagc
     8941 gtatcgttgg ttttcttcac cagatccagc gccgccagac gatcaacgag gatcgcatcg
     9001 atacgcccta cgcgcagatc ctgatatttg gtcgggtcat catcataggt acgcacatcg
     9061 acgccctgaa cattctgccg cagccactct tcatagttgg tgcccagacc gacccccact
     9121 tttttgcctt tcagatcatc ggctgtttta atggtgcctt cgttaccttt tttcaccagc
     9181 gcctgaatac cagaaatggt gtacggggtt gagaaatcgt attttttctt gcgctcatca
     9241 gaaatggtga cctgattaat caccacatca atacgtttag agtccagcga cgccagcata
     9301 ccgtcccatt tggtcggttt tagtgacgcc tcaacgccaa gatgttttgc cagctgttgg
     9361 gcaaattcca cttcaaaacc ggttaatttg ccgtcatccc cctgaaaact gaacggcgga
     9421 taagtacctt ccagccctac cagcagcgtg ccgcgctctt taactttatt aagcagacct
     9481 tcatctgcaa aacttttaac gctcatgccc gcaatcagcg ctacggccat cacacccatc
     9541 aatgcctgac gtcccagatg tgctaatttc atattcaccc cgaatgttgt tatgtctgtt
     9601 tgcagtgtag agccattgtt tgtaaacaca aaaacaactc cgctacatct tattcttatt
     9661 taatatatat cagaagcagg caggctggag gatttctgca caagtccggt acaggtttgg
     9721 cgctgatagt gttgatactt ccgtaacgcg atgcggtaat tgttggtgct ggtggtgggc
     9781 agccacgggg cgaggatttc atcaaggaaa ccgtcctgca gctgatcgag ggaaatattt
     9841 tgctcgtgta gatgattccc caaacggcgc agacgaacga catattcacg caccgtacca
     9901 gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc gaaaaaatca
     9961 ctctgcgttt tgcaatgtaa atcaccgcaa aatcggcaca atgccgccca tgatttttgt
    10021 tccgtttgcc agccattttc atcaagcagc gtgtccaggc gggaaatctc gattttattc
    10081 actattttgc cgtcgcgaac taaggtaatg cgatcgagca atttacggca atgcgcgcaa
    10141 tgggtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct tttcaggtgc
    10201 tgcaccatca ttaagaactc ctggtagtca aagttaaagt gcggcattta ctgacgttat
    10261 aacttaccca gtttagtgcg taaccgttta atagcctggc tgtgtaactg actgacccgc
    10321 gattccccga cctccagcac tgcaccaatc tctttgagat tcagctcttc ctggtaatag
    10381 agggttaata ccagtttttc gcgctccggc aacgtttcga tagcttccat cacccgctgg
    10441 cgcagattac tgtccagtag ttgttgtagc gggttttctc gttgatgatc atcagtaacc
    10501 aattcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag ctggctgtta
    10561 ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg ttccgctacc
    10621 tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc ctgtgccact
    10681 tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag ttcatccagc
    10741 atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg tagggcgtca
    10801 tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc gtcaagttcc
    10861 acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag cgggacataa
    10921 cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga attcacgata
    10981 aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg gtttttaatc
    11041 ggacgattag tgggtgaaat gaggggttat ttgggggtta caggtaaatt ccaggcagaa
    11101 aaaaccccgc cggtggcggg gaagcacgtt gctaacaaat tgcgctttat gttgccggat
    11161 gcggcgtaaa cgccttatcc ggcctacaag aatgtgcaaa ttcaataaat tgcaattcaa
    11221 cttgtaggcc tgataagcgc agcgcatcag gcaatttggc gttgccgtca gtctcagtta
    11281 atcaggttac aacgattaac cctgcagcag agacagaacc tgctgcggta cctggttagc
    11341 ttttgccagc acggagttac cggcctgctg aatgatctgc gctttcgaca tgttggacac
    11401 ttcggtcgca tagtcggcgt cctgaatacg ggactgcgct tcggacaggt tggtagtggt
    11461 gttgttcagg ttggtgactg cggaatccag acggttctgg atagcaccca gggaagaacg
    11521 gaatttgtcg atggagctga ttgcgtcgtc cagggcagca agcgggttgg tcgttgcaga
    11581 accagcactg gtagtctcag tagtgatttt acccgcggag ttcacattta cagcagtacc
    11641 aattgctgga gcataatctt tattggtatc agtcgctgaa gcatacccgg caacagtcac
    11701 agagccgtta tccttgttaa cgctgtaaga gactgtatag tcggcaacgt tagtgatacc
    11761 acctttatca tccacataag acttcgcagc atcactggat tcacccgcgg taaacccaat
    11821 agttttgctc agtacaccgg aattaaaggt aattgaagat gtcgcggcag ccgctttggt
    11881 ttcgctcaat actacatctt tactcactgt agcctgataa gtaatcccac cagggattaa
    11941 cggagctact ggagtggttg tcgcaggagt tgcttttgcg atggtatatt ctgtgccatt
    12001 gaatgtcaga gaggcaccgt cactaccttc gctcgctgct ttgagcagcg ctttcatatc
    12061 agctttagct gcgctaccag cgttgttagt cgtcaagttg cctgcatcgt ctacgtatgc
    12121 ctggcttcca ccgatggtga tattacctgc tgaatccgtt tcgaaagaaa cagtaccatc
    12181 ttttgtggtg taagaaccat taacagattt acccacatca gcatgaaggg tggcagctaa
    12241 cgcatcacgt tttgaagctg aatcagcagc ctgcgccgcg gcgtctacac ccgtaccagc
    12301 agtagattta gtggtagtaa aatcaccaga tttagcgttg taagtataat ctacgccgcc
    12361 aactgtgact ttatcgccat tccctaattt atcgaatgca gcatcggtag ttaacaaggt
    12421 attttcggtt ttcagatcat aaagacctgt cgtggtgttt aacttcgcgc cagcagaagt
    12481 taaatcactt accgttgcag ctttgttggt aatagtacct ttaccattta cgttaaagcc
    12541 attcagaccc agagtatcag aatcgatttt tttcaggtcg atcgtgatgg tttcgccgtc
    12601 attcgcacca acctgaattt tcattgaacc gtctttcgcc agcacgttca cgccgttgaa
    12661 ctgggtctgg ccggatacgc ggtcaatttc atcaagacga gatttgattt cgtcctggat
    12721 ggagtccagg tcagaatcgg agttagtccc tgtagtggcc tgaaccgtca gttcacgaat
    12781 acgctgtaag ttgttgttga tttcggacag cgcgccttcg gtggtctgcg caacagaaat
    12841 accgtcgttg gcgttacggg ccgcctgagt caggccttta atgttagaag taaaacggtt
    12901 agcaatcgcc tgacctgcgg cgtcatcctt cgcgctgtta atacgcaagc cagaagacag
    12961 acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt gagtgatcag
    13021 cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat tgcaagtcgt
    13081 tgattacgta ttgggtttcc acccgtcggc tcaatcgccg tcaaacctgt tatcgtctgt
    13141 cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt cgccgaaata
    13201 ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta aactttgcgc
    13261 aattcaggcc gataaccccg gtattcgttt tacgtgtcga atgataaaag gaaatcgcat
    13321 ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa tccttgatag
    13381 cctcaccgcc gcgcaaaaag cgacgctaac ccccatttca aatcagcaat cgtcgtttac
    13441 cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc agaccgccaa
    13501 tactgcattg tctaaagccg atcttttttc cgccaccagc agcaccaccg cgttcagtgc
    13561 caccaccgcg ggtaacgcca tcgccgggaa atacaccatc agcgtcaccc atctggcgca
    13621 ggcgcaaacc ctgaccacgc gcaccaccag agacgatacg aaaacggcga tcgccaccag
    13681 cgacagtaaa ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag
    13741 cgcggctaac tcgtcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcaggtgt
    13801 tagcgcgagc attattaacg tgggtaacgg tgattatcgt ctgtcagtca catcaaatga
    13861 caccggcctt gacaatgcga tgacgctctc ggttagcggt gatgatgcgc tacaaagttt
    13921 tatgggctat gacgccagtg ccagcagcaa cggcatggag gtctcggtcg ccgcccagaa
    13981 tgcgcagctg acggtcaaca acgtcgccat tgagaacagc agcaacacca tcagcgacgc
    14041 gctggaaaac atcaccctga acctgaacga tgtcaccacg ggcaaccaga cgctaaccat
    14101 cactcaggac acctccaaag cgcaaacggc gattaaagat tgggtgaatg cctataactc
    14161 gctaatagat accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag
    14221 ccagagttct agcaatggcg cactgctcgg cgactccacg ctgcggacga ttcagacgca
    14281 gttgaagtcg atgctgagta ataccgtcag ttcttccagc tataaaacgt tggcgcagat
    14341 tggtatcacg accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc
    14401 tgcactgaaa aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac
    14461 cggcatcacg accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat
    14521 taaagccgct accgatggcg ttagtaagac cctgaataaa ttaactaaag actacaacgc
    14581 cgccagcgat cgcattgacg cgcaggtcgc gcgctacaaa gaacaattta cccaactgga
    14641 cgttttaatg acctcgttaa acagcacaag cagctactta acgcagcagt tcgaaaacaa
    14701 cagtaattcc aagtaatcat tattcatcgg gagacaggtc atgtacgcgg caaaaggcac
    14761 ccaagcctat gcacaaattg gcgtcgaaag cgccgtaatg agcgccagcc agcagcagct
    14821 ggtcaccatg ctatttgatg gagtgctgag cgcactggtt agagcgcgcc tgtttatgca
    14881 ggacaacaat cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa
    14941 cggactgcgg gtgagtcttg atgaagagag caaagacgaa ctaacccaaa acttgattgc
    15001 tctttatagc tatatggtca ggcgtttgct gcaagccaat ttacgcaacg atgtctccgc
    15061 agtcgaagaa gtggaaacat taatgcgcaa tattgccgat gcctggaaag agtcgttact
    15121 ctccccttct ttgattcagg acccagtctg atgaaccatg caccgcattt atatttcgcc
    15181 tggcaacaac tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg
    15241 gacgaactca tcgccagcga aatggcgtat gttaatgcgg tgcaggagat tgcacatttg
    15301 actgaagagg ttgacccgtc caccacgatg caggagcagc tccgcccgat gctgcgcctg
    15361 attctcgaca acgaaagcaa ggtaaagcag ttattacaga ttcggatgga tgaactggcg
    15421 aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttga gtgcctatgg cgatcagggc
    15481 ggctttgtgc tggctccgca ggataacctc ttttgaatct gaatgagtcg atggctcgcg
    15541 aataatccga ttacggctac gcttctaatg ttccccttga atggagtcga agaatgcgta
    15601 atcccacgct gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg
    15661 aattggccga gcgcgccgac ggttttaatg atattggtat caatatggtc tggttgccgc
    15721 ccgcctataa aggcgcatcg ggcgggtatt cggtcggcta cgactcctat gatttatttg
    15781 atttaggtga gtttgatcag aaaggcagca tccctactaa atatggcgat aaagtacaac
    15841 tgctggccgc cattgacgcc ctgaaacgta atgacattgc ggtgctgttg gacgtggtag
    15901 tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgc gttaatgctg
    15961 atgaccgtac gcaaattgat gaagaaatca ttgaatgcga aggctggacg cgttacacct
    16021 tccccgctcg tgccgggcaa tactcacagt ttatctggga tttcaaatgt tttagcggta
    16081 tcgaccatat cgaaaacccc aatgaagatg gcatttttaa aattgttaac gactacaccg
    16141 gcgaaggctg gaacgatcag gttgatgatg aactcggtaa tttcgattat ctgatgggcg
    16201 agaatatcga ttttcgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg
    16261 tgatggaaca aacgcaatgc gacggttttc gtcttgatgc ggtcaaacat attccagcct
    16321 ggttttataa agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg
    16381 tggcggagta ctggtcgcat gaagttgata agctgcaaac gtatattgat caggtggaag
    16441 gcaaaaccat gctgtttgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg
    16501 gacgcaacta cgacatgacg cagattttca ctgggacgct ggtggaagcc gatccttttc
    16561 acgccgtaac cctcgttgcc aaccacgaca cccaaccgtt gcaagccctc gaagcaccgg
    16621 tcgaaccgtg gtttaaaccg ctggcgtatg ctctaatttt gttgcgggaa aatggcgttc
    16681 cttcggtgtt ctatccggac ctctacggtg cgcattacga agatgtcggt ggtgacgggc
    16741 aaacctatcc gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc
    16801 agcgtttcgc ccacggtgta cagacgttat ttttcgacca tccgaactgc attgccttta
    16861 gccgcagtgg caccgacgaa tatcccggct gcgtggtggt catgtcgaac ggagatgatg
    16921 gcgaaaaaac cattcattta ggagagaatt acggcaataa aacctggcgt gattttcttg
    16981 ggaaccggca agagagtgta gtaaccgacg aaaacggcga agcgaccttc ttttgcaacg
    17041 gcggcagcgt tagcgtgtgg gttatcgaag aggtgcttta aattcatccc cggcggcaag
    17101 ccggggagat ttcattacgg caatggcgtc ggtaacggcg ctttatccag cgcagcggca
    17161 cactctgccg tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta
    17221 ccgtcacgct caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatcagac
    17281 atcaatgtca acttgcccgg cacagcaatc acccgctgcc actgacggca atctagggta
    17341 tcgccctctt tagtgacaat caggctgcct atcgcttccg ggctgaccag cgcacgttgc
    17401 ggccctttgg tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg
    17461 ttatagtttt cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt
    17521 tttttcatca ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gccctgatgt
    17581 cgccagcctt tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta
    17641 ccccgaaaa
//
LOCUS       DAZKIF010000100        17258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZKIF010000100 DAZKIF010000000
VERSION     DAZKIF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.100"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            99..353
                     /gene="bssS"
                     /locus_tag="ACHOTW_003388"
     CDS             99..353
                     /gene="bssS"
                     /locus_tag="ACHOTW_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP9845434.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            468..1586
                     /gene="solA"
                     /locus_tag="ACHOTW_003389"
     CDS             468..1586
                     /gene="solA"
                     /locus_tag="ACHOTW_003389"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP9845435.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            1634..1747
                     /locus_tag="ACHOTW_003390"
     CDS             1634..1747
                     /locus_tag="ACHOTW_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP9845436.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2008..2574
                     /gene="yceJ"
                     /locus_tag="ACHOTW_003391"
     CDS             2008..2574
                     /gene="yceJ"
                     /locus_tag="ACHOTW_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP9845437.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2578..3153
                     /gene="yceI"
                     /locus_tag="ACHOTW_003392"
     CDS             2578..3153
                     /gene="yceI"
                     /locus_tag="ACHOTW_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP9845438.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3195..4247)
                     /gene="trhO"
                     /locus_tag="ACHOTW_003393"
     CDS             complement(3195..4247)
                     /gene="trhO"
                     /locus_tag="ACHOTW_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9845439.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4472..5392
                     /gene="lpxL"
                     /locus_tag="ACHOTW_003394"
     CDS             4472..5392
                     /gene="lpxL"
                     /locus_tag="ACHOTW_003394"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP9845440.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5564..6790
                     /gene="mdtG"
                     /locus_tag="ACHOTW_003395"
                     /pseudo
     CDS             5564..6790
                     /gene="mdtG"
                     /locus_tag="ACHOTW_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            6873..7247
                     /gene="msyB"
                     /locus_tag="ACHOTW_003396"
     CDS             6873..7247
                     /gene="msyB"
                     /locus_tag="ACHOTW_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9845441.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7248..7475)
                     /gene="yceK"
                     /locus_tag="ACHOTW_003397"
     CDS             complement(7248..7475)
                     /gene="yceK"
                     /locus_tag="ACHOTW_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9845442.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7648..10191)
                     /gene="mdoH"
                     /locus_tag="ACHOTW_003398"
     CDS             complement(7648..10191)
                     /gene="mdoH"
                     /locus_tag="ACHOTW_003398"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP9845443.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10184..11719)
                     /gene="mdoG"
                     /locus_tag="ACHOTW_003399"
     CDS             complement(10184..11719)
                     /gene="mdoG"
                     /locus_tag="ACHOTW_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP9845444.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12112..13269
                     /gene="mdoC"
                     /locus_tag="ACHOTW_003400"
     CDS             12112..13269
                     /gene="mdoC"
                     /locus_tag="ACHOTW_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP9845445.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13277..14758)
                     /gene="clsC"
                     /locus_tag="ACHOTW_003401"
     CDS             complement(13277..14758)
                     /gene="clsC"
                     /locus_tag="ACHOTW_003401"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP9845446.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14700..15233)
                     /gene="ymdB"
                     /locus_tag="ACHOTW_003402"
     CDS             complement(14700..15233)
                     /gene="ymdB"
                     /locus_tag="ACHOTW_003402"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP9845447.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15328..15639)
                     /gene="ymdA"
                     /locus_tag="ACHOTW_003403"
     CDS             complement(15328..15639)
                     /gene="ymdA"
                     /locus_tag="ACHOTW_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP9845448.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15760..16092)
                     /gene="csgC"
                     /locus_tag="ACHOTW_003404"
     CDS             complement(15760..16092)
                     /gene="csgC"
                     /locus_tag="ACHOTW_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP9845449.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16151..16609)
                     /gene="csgA"
                     /locus_tag="ACHOTW_003405"
     CDS             complement(16151..16609)
                     /gene="csgA"
                     /locus_tag="ACHOTW_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP9845450.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(16650..17105)
                     /gene="csgB"
                     /locus_tag="ACHOTW_003406"
     CDS             complement(16650..17105)
                     /gene="csgB"
                     /locus_tag="ACHOTW_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP9845451.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4328 a         4377 c         4095 g         4458 t
ORIGIN      
        1 gtgattaccc ttatttattg gtatactgaa gttgcttcag ataaaaacgc attgaacctc
       61 gaataacgtt gtctagtaac acgaattagg gggccatgat ggaaaaaaat aatgaagtca
      121 ttcagactca tccgctcgta gggtgggaca tcagcaccgt tgatagctat gatgcgctga
      181 tgttgcgttt gcactaccag accccaaata agtccgagca ggaagggact gaagttggtc
      241 agacgctctg gttaaccact gatgttgcca gacaatttat ttcgatatta gaagcaggaa
      301 tcgccaaaat tgaatccggt gatttcccgg taaacgagta tcggcgtcat taacatgaca
      361 tgaccatcca cccaaaaggc acctcaccgg tgcctttacc atttctgggt tgtatatctg
      421 cattcagtta attactctgc ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc
      481 tcatcattat tggcagcggt tccgtaggcg ctgccgccgg gtattatgca acccgcgccg
      541 gtttaaacgt gcttatgacc gacgcccata tgccaccaca tcaacacggc agccaccacg
      601 gcgatacgcg attaattcga catgcttatg gtgaaggcga aaagtatgtc ccgctggtcc
      661 tccgcgcaca aacgctgtgg gatgacctct cccgccacaa cgaagatgat cccatttttg
      721 tacgctctgg tgtcattaac cttggcccgg ctgactccgc atttctcgcc aacgtcgccc
      781 acagcgccga acagtggcaa ctcaacgttg aaaagctcga tgcgcaaggg attatggccc
      841 gctggccaga aatacgcgtc ccggacaact acatcggctt atttgagact gattccggtt
      901 ttttgcgcag cgaactggcg attaaaacct ggatccaact ggcgaaggaa gcgggctgtg
      961 cgcaactgtt caactgcccg gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg
     1021 aaacggctga cggtgagtat caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa
     1081 aagacctgct cccggagctg cctgtccagc ccgtacgtaa agtatttgcc tggtatcagg
     1141 ccgatggccg ctatagcgtg aagaataaat tcccggcgtt taccggtgaa ctgcccaatg
     1201 gcgatcaata ttatggtttt ccggcagaaa acgacgcgtt gaagattggc aaacataacg
     1261 gaggccaggt tatccattca gcggatgaac gtgttccgtt tgcggaagtg gtcagcgatg
     1321 gttcggaagc cttcccgttc ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg
     1381 gcgctgcctg cacctatgat aattcgcctg acgaagattt tattatcgat accctacccg
     1441 gccacgataa tacactgctc attaccggcc tgagtgggca cggttttaaa tttgcgtcag
     1501 ttttagggga aatagctgcc gattttgcgc aagacaaaaa aagcgatttt gatttgacgc
     1561 cattcagcct ttcccgcttc caataatcat cattacggcc tccagaatga ggccgttttt
     1621 acaggagtat ttaatgcgtc ggctgttgca ctatctcatc aataatattc gcgagcatct
     1681 gatgctatat ctttttctct ggggattgtt ggcaattatg gatctgatct atgtgtttta
     1741 tttttagttt tccgcactga cataaactga caaatattta atctcccttt catttagcgt
     1801 gaaaaattga atgagattat tcctgcttct acagcactca ccagacatct ctgatgctga
     1861 acccactaaa cttatgaaga ttctttcatt attaattcaa aattattgaa ataaaaataa
     1921 atatcattca atttgttatt tttgtgttgc aattcattct ccgacacgcc attttctttc
     1981 tctcacaaat tcgttttgga tgcacttatg tcattcacaa atacccctga acgctatggt
     2041 gttatatctg cggccttcca ttggctaagc gcaattattg tctacggcat gtttgcttta
     2101 ggtctgtgga tggtcacgct cagttattac gatggctggt atcacaaagc acccgaacta
     2161 cataaaagca tcggtatttt gttaatgatg gggctggtta ttcgcgttct gtggcgtgtc
     2221 atatctccac cgccgggtcc gctgccgagt tattcgccca tgactcgtct tgccgcaaaa
     2281 gctggacatc tcgcattgta cctattgctg ttcgccatcg gcatcagcgg ctatctgatc
     2341 tcaactgccg atggtaaacc gatcagcgtt tttggctggt ttgacgtccc cgcgaccctt
     2401 gccgacgctg gcgcacaggc agactttgcc ggtgccctgc atttttggct cgcgtggagc
     2461 gtcgtagtac tgtccgttat gcacggattt atggccctga agcatcattt catcgataaa
     2521 gacgacactc tgaagcgcat gctgggaaag tcgtcatctg actatggagt ataaaaaatg
     2581 aaaaaaagcc tgcttggttt aaccttcgcg tccctgatgt tctctgccgg ttcagcggtt
     2641 gccgccgatt acaaaattga caaagaaggt cagcacgcct ttgttaactt tcgcatccag
     2701 catcttggct atagctggtt atatggcacc tttaaagatt tcgacggtac ttttaccttt
     2761 gatgaaaaaa atccagctgc cgataaagtg aatgtgacaa ttaacaccac cagcgtcgat
     2821 actaatcacg ccgaacgtga taaacatctt cgcagtgcag atttcctcaa taccgcaaaa
     2881 tatccacagg caacattcac ctccaccagc gtgaagaaag acggtgacga actggatatt
     2941 accggcgatc tgacgctgaa tggtgtaacc aaacccgtca cgctggaagc gaaattaatt
     3001 ggtcagggcg acgacccatg gggtggtaaa cgtgcaggct tcgaggccga aggcaaaatt
     3061 aagctcaagg acttcaatat caagacagat ttaggtccag cttctcagga agtggatctg
     3121 attatttcag tggaaggcgt acagcagaag taagagtgaa atgttgccgg atggcacgca
     3181 tccggcaatg atatttattc tgttggatca ggaatgccca gtgttgtatt cagacgtcca
     3241 cgagacttat taaagatctt attgccattt tcacgtcctg cccgacggcg tcgctgttct
     3301 tctggcggta acgcgctttc ttcgcagcaa atttcactac aacaaccttt gtatttttcc
     3361 gcacatactg gacactgaat aaacagcagg tggcagccat catttttaca gttggtatgg
     3421 ctgtcgcacg gcgcaccgca ctggtggcaa tgcgcgataa tctcatcaga tatacgttcg
     3481 cccatccgct cgtcaaaaac aaaatttttg ccaataaaac gcaccggcaa gccctgctcg
     3541 cgcgccttac gggcgtattc aataattcca ccctcgatat gccagacttt attgaatccg
     3601 ttatgtttca tccaggcact ggccttttca caacgaatgc cgccggtgca gtacatgacg
     3661 atttttttat ctttatgtgc ctgcatcatc tcgactgctt ttggcagctg ctcacggaag
     3721 gtatctgccg gaatttccag cgcgttttca aagtgcccca cttcatactc atagtggtta
     3781 cgcatgtcga taaacagtgc atcgggatcg tcaagcatgg cgttcacttc cgccgcttgc
     3841 agatactcac caacattgct ggcatcaaag tgaggatcgt caataccgtc ggcaacgata
     3901 cgatcgcgga ccttcatgcg cagtacccag aaggatttcc cgtcatcatc caacgcgata
     3961 ttcaggcgta agccatctaa agccgagtcg aaggcataga gctgcgcgcg aaatgtttta
     4021 acattgctcg caggtacgct gatttgcgcg ttaatgccct catgcgccag atacactcgc
     4081 ccaaaaacat tcagcgcggt aaacagctga tataaagcgt cacgggtcgc cttaggatcg
     4141 gcgatgtgga aatacttgta aaacgaaatg gtggttcgcg gttcgctctc agccaacatt
     4201 ttggctttta acgcgtcgtt ggaaatgcgg ttgtgtaaca ctggcatggt gtacggttcc
     4261 tgcgagatgg gaaagtaaaa atccgcggca tgatatagca attatcgata attaacatcc
     4321 acacatttta cgctacattt gcgcattaaa aattatttgt tatttacaag cgcggcaatt
     4381 tcgcccagtc ttcagccaca aattttggtt gcgggcgaaa aaatgcgaca atacatacaa
     4441 ttgcccgaat aggttgaaaa acaggattga tatgacgaat ctacccaagt tctccaccgc
     4501 actgcttcat ccgcgttatt ggttaacctg gttgggtatt ggcgtacttt ggttagtcgt
     4561 gcaattgccc tacccggtta tctaccgcct cggttgtgga ttaggaaaac tggcgttacg
     4621 ttttatgaaa cgacgcgcaa aaattgtgca tcgcaacctg gaactgtgct tcccggaaat
     4681 gagcgaacaa gaacgccgta aaatggtggt gaagaatttc gaatccgttg gcatgggact
     4741 gatggaaact ggcatggcgt ggttctggcc ggaccgccga atcgctcgct ggacggaagt
     4801 gatcggcatg gaacacattc gtgacgtgca ggcgcaaaaa cgcggcatcc tgttagttgg
     4861 catccatttt ctgacactgg agctgggtgc gcggcagttt ggtatgcagg aaccggggat
     4921 tggcgtttac cgcccgaacg ataatccact gattgactgg ctacaaacct ggggccgttt
     4981 gcgctcaaat aaatcgatgc tcgaccgcaa agatttaaaa ggcatgatta aagccctgaa
     5041 aaaaggcgaa gtggtctggt acgcaccgga tcatgattac ggcccgcgct caagcgtttt
     5101 cgtcccttta tttgccgttg agcaggctgc gaccacgacc ggaacctgga tgctggcacg
     5161 gatgtccggc gcatgtctgg tgcccttcgt tccacgccgt aagccagatg gcaaagggta
     5221 tcaattgatt atgctgccgc cagagtgttc tccgccgctg gatgatgccg aaactaccgc
     5281 cgcgtggatg aacaaagtgg tcgaaaaatg catcatgatg gcaccagagc agtatatgtg
     5341 gttacaccgt cgctttaaaa cacgcccgga aggcgttcct tcacgctatt aaatctccca
     5401 tgccggatgc ttcagaatgg catccggcat taccacagca aatccccctg atttagcgat
     5461 aaaagctctc tggattgcgc cccctggaag tcgggcgcat aattagtgtg cttatctttt
     5521 cttcttatgt tcaccgcgcc tggcgcacca acagcggatt gctatgtcac cctgtgaaaa
     5581 tgacacccct ataaactgga aacgaaacct gatcgtcgcc tggctaggct gttttcttac
     5641 cggtgccgcc ttcagtctgg taatgccctt ctaacccctc tacgttgagc agcttggcgt
     5701 taccggccac tccgccctga atatgtggtc cggtattgtc ttcagcatta catttttatt
     5761 ttcggccatc gcctcaccgt tttggggtgg actcgccgac cgtaaaggcc gaaaactcat
     5821 gctattacgc tctgctctcg gcatgggcat cgtgatggtg ttgatggggc tggcacaaaa
     5881 tatctggcag tttttgatcc tgcgggcgct tcttgggtta cttggcggat ttgtccccaa
     5941 cgctaatgct cttatcgcca cacaagtacc gcgtaataaa agcggctggg cactgggtac
     6001 gctctccaca ggcggcgtta gcggtgcgtt gctcggccca atggctggcg gcctgctcgc
     6061 cgatagctac ggcttacgtc cggtattctt tattaccgcc agtgtgctca tactctgctt
     6121 tttcgtcacc ctgttttgca tcagagaaaa attccagccg gtcagcaaaa aagagatgct
     6181 gcacatgcgg gaagtggtga catcacttaa aaacccgaaa ctggtactca gcctgtttgt
     6241 caccacgtta atcatccagg tggcgacggg ctcaattgcc cccattctga cgctgtatgt
     6301 ccgcgaactg gcgggtaacg tcagtaacgt cgcctttatc agtggcatga tcgcctcggt
     6361 gccaggcgta gctgctctgc tgagtgcacc acgactcggc aaacttggcg atcgaatcgg
     6421 acccgaaaag atcctgatta cggcgctgat cttttctgta ctgctgttga tcccaatgtc
     6481 ttacgttcag acgccattgc aacttgggat tttacgtttt ttgctcggtg ccgccgatgg
     6541 tgcactactc cccgccgtac agacactgtt ggtttacaac tcgagcaacc aaatcgccgg
     6601 acgtatcttc agctataacc aatcgtttcg tgatattggc aacgttaccg gaccattgat
     6661 gggagcagcg atttcagcga actacggttt cagagcggta tttctcgtca ccgctggcgt
     6721 agtgttattc aacgcagtct attcatggaa cagtctacgt cgtcgtcgaa taccccaggt
     6781 atcgaactga tttttcgcct ttcatacttg caaaagcgga gaatcagcta tccttttccc
     6841 tgaaacctca tcaactcaaa gggagaatcg tgatgaccat gtacgcaacg cttgaagaag
     6901 ccattgacgc tgcacgcgaa gaatttcttg cagacaaccc cggcatcgac gccgaagatg
     6961 cgaatgtgca acagttcaat gcccaaaaat acgttttgca ggacggcgac atcatgtggc
     7021 aagttgagtt ttttgccgac gaaggggaag aaggtgaatg tttacctatg cttagcggtg
     7081 aagccgcgca aagtgttttt gatggcgact atgatgagat agagatccgc caggagtggc
     7141 aggaagagaa tacattacat gaatgggacg agggggaatt tcagcttgag ccaccgctgg
     7201 ataccgagga aggacgcgca gcagctgatg agtgggatga acgttaatta ctcatacggg
     7261 ccatgatgaa tgtcgatcgg cagcagtaaa gtatcaaaaa ccagggagaa tggcagatca
     7321 aggattgtga cataacgcca ggcggagtca cgcacatccc attgcacacc gggataatat
     7381 tggttgccat gcccctgccc cggtatagtg cggctaatga tgctgccaca gccactcagc
     7441 aaggtcacca tgatgctcac cacaattaaa cgcatttgat acctcttccg taaaaaaata
     7501 ccggcataac gccggtattt ttattggctc gttgtttttg ccagatgtgg cgtgaacgcc
     7561 ttatccgacc tacaaaatct tgcaaaatca acacattgca ggagcgatgt aggcctgata
     7621 agcatagcgc atcaggcaac tacgttttta ttgcgaagcc gcatccggtt tacgcaatgc
     7681 cagtggattg agctttatcc cttcgtaata actcacccat gaagaatatc tctcagggga
     7741 attccagacg cggaaatgca gacgggccat cgtcaccgga tcgcttagca gcacaaggcg
     7801 acgatcgcga ttcagcttct ctggcgtctc gttcagcgcc tgttcaacat ggcggtcacg
     7861 ggcgatttcc agcaccttac tggcgcggtg acgcgcggtc gccattgcgg ttgccagagc
     7921 gttaaatgac gggttaaaca ctgcgtgcat aaaaccatca tcaagggagc gttgacggtt
     7981 catctcaagg aaccgatcgg tatcaaccag tacctgcggc ggtgaatact cttccgggat
     8041 caggaacagt ttccagcgtt tggtgcgcag accaacggtg gcacggctgg aaatcaccga
     8101 aacaaacggt gacaggatca acgagaagac aatcggtgcc agccagaaca ggaaacgcag
     8161 atccaaccac gccatcccga cagcccacac taaccctaac agcagctgtg agccgtggcg
     8221 tttgaacgct tcaccccagg aagtggagtc atcatcacgc tgcggtgaat tccacaccac
     8281 ttcccagcca aggaacgcgc tgacgacgaa gaccgtatgg aacagcatgc gtaccggagc
     8341 cagcagcacg gaaaacagca cttccagcag caacgataat gtaacgcgcc agaagccgcc
     8401 atattctttc gttcctttac accaaataag caaaatgctc aatagctttg gcaggaacaa
     8461 cagcaccatg gtcgaagcaa aaagtgcaat cgccagctca ggacgccact gcggccacac
     8521 cgggaacaac tgccgtggtt gcaggaagta ttgcggttcg gtcaacgcat gcactacctg
     8581 caatgcagta gagagcgcga ggaacataaa ccacagcgga gcggagagat aagacatcac
     8641 gcccgtcagg aataccgcac ggtgaaccgg gtgcataccc ttcaccagga acagacggaa
     8701 gttcatcagg ttaccgtggc accagcggcg gtcacgtttt agctcatcaa gcaagttagg
     8761 cggtaattct tcataagaac ccgggagatc gtaagcaatc cagactcccc aacctgcacg
     8821 gcgcatcaac gccgcttcca cgaagtcatg tgacaggatt gaaccggcaa aggaaccttc
     8881 gcccggcagc ggggccagtg cacagtgctc gataaacggt ttcacgcgga taatcgcgtt
     8941 atgcccccag tagtgcgact cgccaagttg ccagaagtgc aaaccggctg taaacagtgg
     9001 cccatacacg cgggtcgcga actgctgaca gcgtgcatac agcgtatcca taccggacgc
     9061 tttcggcgac gactgaatga tcccggcgtt cgggttggct tccatcaggc gcaccaggcc
     9121 gcacaaacaa tcaccggtca ttaccgagtc agcatccagc accaccatat agctgtactg
     9181 gctgccccag cgacggcaga agtcatcgat attaccgctt ttacgcttca cgcgacggcg
     9241 gcggcggcga tagaaaatct gaccttcgcc accgacttca gcgataagct ccatccaggc
     9301 tttttgctct gcgacgcaga tatccgggtt gtaactgtca ctcagaatgt agacatcaaa
     9361 gtgtttggca ttcccggtgg cttttactga ttcccacgtt gcacgcaagc cagcaaaaac
     9421 acggttcacg tcttcgttac agataggcat gatcaacgcc gtgcgatgct ccgggtttaa
     9481 tggttcatcg ccaactgttg acgcagatat actgtattta tcgcgaccaa taagtagttg
     9541 caggaagccc attaacgccg tccagaatcc ggcggacacc caacagaaca gtaccgcaaa
     9601 gaggatcagg ataccggttt gcagcatata aggcagaagc tgcataaagg aaacccacaa
     9661 atcctgacca accatatcca taggattaat cagcgcccaa ccctgataag gaagaatggt
     9721 cttcatatac caggtcgcga cgacagtttg cgcgagcgtc aggatcaaca gaatgtaacg
     9781 gcggatggta ccgacggtac gccacttttg ctcgctctcc tgctcttctt tggtcaaacg
     9841 agccagatag cgcggtgtta catcgcgtcc acgcaggcga tcccagaaac ggcctaccgg
     9901 gttggtacgc cacgggtcgg gaaacatcga ggagcgtttt gcttctggca tcgccttaag
     9961 ctgatcgcgc ccttcgtcat ctttaattaa ctgtccatca gcaagtgaat ctggccaggc
    10021 ttgttccaga cgcgccttta ccgagccttg cggggagtca tcctcccgcg cccaggtgcg
    10081 gtgttcggca tccagcgcct gatgaacggc gcggatatca gtcttcggca atgccgcttt
    10141 ctcgcttgcg gcgatgggca ttgcgtcaat gtactcagtt gtcttattca ttggcaggta
    10201 actggtagct ccaggtttca ctcaacgtct gatcggcatt caccagcgca gcgcgcattt
    10261 cagtggtttt cttggcatct ttcactttca cacgcatcac cagacgccag cctttggtga
    10321 ccgggttata gcgcaccgtg ctttcaacta tctcaccatt atcaccaatg ctggtttgcg
    10381 ctgtgaccgg ggtatcctct ggcagttttt tcatctctgc gccggtaaaa tcgaccacaa
    10441 aggcgatagt accgtcaggc tggcgaatca ggttcgactg cttcacatcc cccgttgaac
    10501 gacgcgtttg ttgcacccat gcgttatctg gcgcatgcag tttgtcttca tcacggctga
    10561 aggtgatggt gtatttaaag ttcatctctt tacccggctc cggcagctga tccggcgtcc
    10621 agtaagcgac gatgttatcg ttagtttcat cgttggttgg aatttccacc agctcaacgc
    10681 tgcctttacc ccactcccct ttcggagtca cccatgcgct tggacggaga tcgtaacgat
    10741 catcgagatc ttcaaagcgg gagaaatcac gaccgcgctg caacagacca aagccttgcg
    10801 ggttttccat ggagaagctg ctgaccgcta aatgtttcgg gttattcaac ggacgccaga
    10861 tccattcgcc attaccggca tggatagaga gaccgttaga gtcgtgcaac tccggacgat
    10921 agttatttgc tggcgacggt tggttcggcc caaacaggaa catactggtt aacggtgcaa
    10981 cccccagttt gccgacttta tcgcgcagat agattttcga ctgcacatcc acaaccgtgt
    11041 cacgccctgg cataactacg aatttgtaag cacctgtcgc gcgcggcgag tcaagcaatg
    11101 cataaatggt taaacgttta tcagttggtt ttggacgctc gatccagaac tctttgaagc
    11161 gtggaaattc ttcacccgat ggcaaggcgg tatcaattgc cagaccgcgg gcagaaaggc
    11221 cataaacctg acctgcacca atcacgcgga aatagctggc cccgagcatg ctgacgattt
    11281 catcgttttt atctttgctg ttgatcgggt aaagcacttt aaaaccggca aaaccaaggt
    11341 cttttaccgt gtctttgtca tgctgaacat cgccgaaagt gaaataatcc gggctgtatt
    11401 tgattcgttt gactgcggtg gcagtcactt catttatttt gaccggggta tcgaagtaca
    11461 taccctgatg gtagaactcg agtttgaatg gggtcttcag attgttccag tacgctttgt
    11521 catgattaaa ctggatctgc tgatagtccg cgtatttcat atcgcggaaa acggagggca
    11581 agttgctttt gggcgcctca tagcctttcc cggctaagga ttgagcttgc tttgcgacat
    11641 catcaatact gaaagcccag cttgaagatg tatacagggt taacattact gcagcactca
    11701 accaacgcat tttcatcatt tgtagtttat gtttcataat tagtaagcac ttcccccttt
    11761 gtgtgcttat atcgatccga tctattttaa tggataacca gccattccga caacagaatc
    11821 ccggtatcgt tcagcacgct ggctcatgga ttaagcacct gattgcaacc gttttcactt
    11881 tccgtgcttc tcctaaagac agaagcatcg acttcgtgta gggtttgcca gcaaatttat
    11941 ttatcaaccg tcttacagac gcttccattc tggttagtgg tagcgatgat gctatgcata
    12001 aaaacttgaa gcctgcgcct ggcgcggtca ggtcggtgtc gctatggcaa caagcctaaa
    12061 ggcataaccc gacagggata agacgaaaaa tccgagatta gttaaccata tatgaatcca
    12121 gtacccgcgc aacgtgaata tttcctcgac tccatccgcg cctggctgat gttgttaggg
    12181 atcccttttc atatttcttt aatctattcg agccatacat ggcatgtgaa tagcgccgaa
    12241 ccgtcattgt ggctgaccct ttttaatgac ttcatccact cgttccgcat gcaggtattt
    12301 ttcgttatat ccgggtactt ttcctacatg ctttttttac gctatccctt gaaaaaatgg
    12361 tggaaagtac gtgtcgaacg tgtagggatc ccgatgttaa cagccatccc cctactgaca
    12421 ttgccgcaat ttattatgct gcaatacgtc aaagggaaag cggaaagttg gcctgggctg
    12481 tcattgtatg acaaatataa tacgttggcc tgggaattaa tatcacatct gtggttttta
    12541 ctggtgttag tggtcatgac gacgctgtgc gtatggatat ttaaacgcat cagaaataat
    12601 ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg taaaactatc ggtgattttt
    12661 ttgtgcctcg gcatcggtta tgcggtaata agaagaacga tttttattgt gtatccaccc
    12721 attctgagta atggcacgtt caattttatt gtcatgcaaa cgctatttta tttaccgttc
    12781 tttatcctcg gcgcactggc tttcattttc cctcatctta aagccttgtt taccacgccg
    12841 tctcgtggct gtaccctcgc tgcagcattg gcgtttgtcg cttatttact caaccagcgc
    12901 tatggcagtg gcgatgcctg gatgtacgaa accgagtcgg tgatcaccat ggtcctcggt
    12961 ctgtggatgg tgaatgtggt cttctccttt ggccaccgtt tgcttaactt ccagtcagcg
    13021 cgggtgactt attttgttaa cgcatcgctg tttatctatc tggttcacca cccgttaacg
    13081 ctgtttttcg gcgcatacat tacaccgcac atcacctcca actggcttgg ttttctctgt
    13141 ggcctgatat tcgtagtagg gattgcgata attctgtatg aaattcattt gcgcatcccg
    13201 ttactgaagt ttttgttctc tggtaaaccg gttgttaagc gtgagaacga taaagcacca
    13261 gcccgttaag ccacatttac aataaccatt ccacgggcaa tatcgacgcc agtctgacca
    13321 taacccgctt ccagaaactg gtagcgggtt cttttttgag aacaatctct ttctttgcat
    13381 gacgatcaac ccagttgatc cgtccccacc tgtccagacg gagctgccag gccgcatcat
    13441 actggctctg aataaagcgt ttatcaatta actgagccag cgtttcgctc tctatcacga
    13501 agcccatttc agtattgagc aatgttgaac gcggatcgaa attgaaagaa ccgataaaca
    13561 ccgttttacc atcgatgcta aaggttttag catgcaggct ggcaccggaa ttaccggtta
    13621 tgccgcgatc gtgtaacgta ctactttgtt cacgcgtcgg cttgagttca tataattcca
    13681 cgccatagcg gagcaatttt ttgcgccagc gcgcgtatcc ggcatggacg acagcaacat
    13741 cgttagcggc aagagaattg gttaggatcg caatctttac cccttttctc accatccgta
    13801 agagttgcgc cacacctgcg cgtgtcggta caaaatagga agagataata tcgatgcgtt
    13861 cactgggtga gcccatgata tcgaacaggc gctgcggtaa cagtgaatgc cgttttgcct
    13921 tgccctcccc tttcgccgga tcatcactta ataaacgtgt tttcgcccag ataagcggca
    13981 acgttccatc aaccagatga tttataaacg gactggattc cattttgcgt aagtaacgat
    14041 gcgtcatggc atcgttatgc caggaggcgg gtaactcgat gcgatccgcc atttcacctt
    14101 ccgggacatc cagcacctgc tgtaagggtg aaaccgattt gcaataccag tagcgggcga
    14161 aatcatcggc aacgtcctct actacgggtc ctattgccat gacatctaaa tccgaaaaaa
    14221 gtggctcttc ccctgcccca aaataggcat caccaatatt tcgcccccct accaacgtca
    14281 ccacgccatc gacagtgaaa cttttattgt gcatacggcg attgagacgg gaaaagtcgg
    14341 tgatataacc aagcggacgc agcaagcgaa acgagaaagg attaaaaagc cggacttcaa
    14401 tgcggggatg gctgtcaagc aagcgtaaaa tgtcgtcaag tccgggcgta ttgttgtcgt
    14461 ccagcaacaa acggacgcga acgccacgct ttgctgcggc taacagggcg gaaaacagta
    14521 accgacccga catatcgtcc tgccagatgt aatactgaac atccagcgta tgctctgaca
    14581 tttcggccag acgataccgg gcggcaaacg catccagact tttctccaga gggaaaagac
    14641 cacactgacc gggatgttgc gaacacagtg gcagcaccgc gctcgccagc cggggcaaat
    14701 cattcatctc cttgttgggt aaggagtctt tcgtagaggt gggcgttttc ttcatcataa
    14761 cagacaaagt atacctgttc gggtaaagcg tgacgggtaa taaattctga aacggttttt
    14821 actgcgattt cagccgctgc cgcacgaggg taaccataaa ccccagtact gattgcagga
    14881 aaagccactg acgtatagct gtttgccgcc accagtcgta ggctattgag ataggcatcc
    14941 tgcaaaagct ggtcttcgtt ttgttcacca ccacgccaga ctggcccgac ggtgtgcact
    15001 acggctttag caggaagatc gcctgcaagc gtaataacgg catgccccgt agggcaatcg
    15061 ccctgctgtt gcctgacttt taaacaagca tccagcaggg ccggacccgc tgcgcgatga
    15121 atggccccat cgacgccgcc acctcccatt aatgaaggat tagccgcatt cacaatcaca
    15181 tcaacggcca gtttggtaat atcaccctgc acaacatgaa tacgcgtttt cataacgcct
    15241 ccttacacct tcttgtttct taagtgtaca gcaggctttt tggttaaatc gcatttttct
    15301 gctcagcaac gtgagttgtt cagaaactta gttatattcg atgttcatta cagccagctt
    15361 tttctgttta tcaagatact gcacttttac tgaggctatt tgcgcgtttt tgacattgcc
    15421 gctcattaac gctttgctgg agtaggtttt acccggaaca gaaccattct gtgggcagtt
    15481 catttctacg ggtgatgact gggtgctgac gtcacatgcc ggttccacaa tttggccata
    15541 aaaatggatc acgccccctt gcgcggtgct tcctgcgatg gcaataaaag gaaaaaacaa
    15601 actgccgagc ataagagagt ttaaaaaagg tctgaacatt tattctattt cctcaatgat
    15661 tagtcatcct tgagggttgt gttatccata ctttcaggtt ggctgattcg cagcagacca
    15721 ttctctccag attcatctta tgctcgatat ttcaacaaat taagactttt ctgaagaggg
    15781 cggccattgt tgtgataaat gaagtgactg tccatcagaa acagtaacaa ctattttcac
    15841 ccgatcgtcc ggggaaatat ttaaactcaa cttcgtcaaa gcaatgggtt gattagcagg
    15901 caatgaaagg gtcttttctt gcttcgtctg actttgccct gaactgcctt cgcgcaggga
    15961 caatatttgt actctgcaca gacaagattg agtaagagtg acttcaggaa taatggtgta
    16021 catatcccct tgctgggtcg tattaaaggt tatctgactg gaaagtgccg caaggagtaa
    16081 taacgcattc atattcttct cccgaaaaaa aacagggctt gcgccctgtt tctttaatac
    16141 agatgatgta ttagtactga tgagcggtcg cgttgttacc aaagccaacc tgagttacgt
    16201 tgacggtgga attagatgca gtctggtcaa ccgctgcacc gttgccgcca ccgaattgtt
    16261 taactgtcat atgagagtct ttaccgttcc actgatcaag agtggcgctg ttaccaaagc
    16321 cacgttgggt cagatcgatt gagctgtcat ctgagccctg accaacatct gcaccgttac
    16381 caccaccatg ctgggtaata gtaagatcag agttacgagc atcagcttgc agagcaagtg
    16441 cagagttacc accaccgtac tgataaatat tcagctctga attcgggccg ctgttattac
    16501 cgccaccacc gtggttaccg ccaccgccgc cgtactgagg aacaacacct gccagagcgc
    16561 taccggagaa tacgattgct gcaattgctg ctacttttaa aagtttcatg taaaaccccc
    16621 atcggattga tttaaaagtc gaatggaaat taacgttgtg tcacgcgaat agccatttgc
    16681 gactgtctct gcactacaat tgccgttttt tgagtaccat actgtgtaat atttgcttta
    16741 ttaccagaac ctttctggat aatcatcgca gtattaccat aagcaccttg cgaaatactg
    16801 gcatcattgg cactgcccgc ctgatcaata tatgcaaggt tataatctcc tgtctggtca
    16861 atctttgccc ggttgctact accttcttgc gcaacaaccg ccaaaagttt tgagcctccc
    16921 tgccgtaact gagcactatt attagtccca gcttgaccaa ttatggctgc ctgattaaat
    16981 gaagacttac tcaattcatt taccgcgaag ttatattctg aattagctaa atcataacct
    17041 gctgcggctg caatcccagg cgcacccagt attgttaaca tcataaataa caatttgttt
    17101 ttcatgttgt caccctggac ctggtcgtac atttaagaaa ttaaatcatt tcaacttggt
    17161 tgttaacgca acctatattt tgttaacgct gcgttacgat ggaaagtatg tctgcggaaa
    17221 tatttttaat aactcacccg cgcgttgtat ttttcttt
//
LOCUS       DAZKIF010000101        17124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZKIF010000101 DAZKIF010000000
VERSION     DAZKIF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.101"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            302..1201
                     /gene="yegS"
                     /locus_tag="ACHOTW_003407"
     CDS             302..1201
                     /gene="yegS"
                     /locus_tag="ACHOTW_003407"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP9845452.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1283..2056)
                     /locus_tag="ACHOTW_003408"
     CDS             complement(1283..2056)
                     /locus_tag="ACHOTW_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9845453.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(2162..3202)
                     /gene="gatD"
                     /locus_tag="ACHOTW_003409"
     CDS             complement(2162..3202)
                     /gene="gatD"
                     /locus_tag="ACHOTW_003409"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9845454.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3250..4605)
                     /locus_tag="ACHOTW_003410"
     CDS             complement(3250..4605)
                     /locus_tag="ACHOTW_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9845455.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4609..4893)
                     /gene="gatB"
                     /locus_tag="ACHOTW_003411"
     CDS             complement(4609..4893)
                     /gene="gatB"
                     /locus_tag="ACHOTW_003411"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP9845456.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4924..5376)
                     /gene="gatA"
                     /locus_tag="ACHOTW_003412"
     CDS             complement(4924..5376)
                     /gene="gatA"
                     /locus_tag="ACHOTW_003412"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP9845457.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5386..6648)
                     /gene="gatZ"
                     /locus_tag="ACHOTW_003413"
     CDS             complement(5386..6648)
                     /gene="gatZ"
                     /locus_tag="ACHOTW_003413"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP9845458.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6677..7531)
                     /gene="gatY"
                     /locus_tag="ACHOTW_003414"
     CDS             complement(6677..7531)
                     /gene="gatY"
                     /locus_tag="ACHOTW_003414"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP9845459.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7830..8882)
                     /gene="fbaB"
                     /locus_tag="ACHOTW_003415"
     CDS             complement(7830..8882)
                     /gene="fbaB"
                     /locus_tag="ACHOTW_003415"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP9845460.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9139..10416
                     /locus_tag="ACHOTW_003416"
     CDS             9139..10416
                     /locus_tag="ACHOTW_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP9845461.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10413..11417
                     /locus_tag="ACHOTW_003417"
     CDS             10413..11417
                     /locus_tag="ACHOTW_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP9845462.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            11414..12379
                     /locus_tag="ACHOTW_003418"
     CDS             11414..12379
                     /locus_tag="ACHOTW_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP9845463.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12353..13099)
                     /locus_tag="ACHOTW_003419"
     CDS             complement(12353..13099)
                     /locus_tag="ACHOTW_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9845464.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13151..13969)
                     /locus_tag="ACHOTW_003420"
     CDS             complement(13151..13969)
                     /locus_tag="ACHOTW_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP9845465.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(14034..14834)
                     /gene="thiD"
                     /locus_tag="ACHOTW_003421"
     CDS             complement(14034..14834)
                     /gene="thiD"
                     /locus_tag="ACHOTW_003421"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9845466.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14831..15619)
                     /gene="thiM"
                     /locus_tag="ACHOTW_003422"
     CDS             complement(14831..15619)
                     /gene="thiM"
                     /locus_tag="ACHOTW_003422"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP9845467.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15654..15750)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15953..16192
                     /locus_tag="ACHOTW_003423"
     CDS             15953..16192
                     /locus_tag="ACHOTW_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HFP9845468.1"
                     /translation="MTETQIYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKE
                     FCEGVGLKASFATEFSKMRNLTERLKAAGLDTTKL"
BASE COUNT         4339 a         4371 c         4056 g         4358 t
ORIGIN      
        1 atttcctaca taagtaggaa ttacggacat ggaggccctt ccgggtaact ccatggaggc
       61 tttaatatat tatattgaag aagccactgt ttagttgaat attaggtata tgctcttttt
      121 tgaaatttat cggtggctgc cgttagtatt cgctgtcccc attgcaagct cctggtggta
      181 accactgaat cctccatact tgaactgact atttatcctc cgactttcat cctgttctga
      241 ctccaccttt tcttttctgc tctacactat ctatagacca atcataaagg cacatacgat
      301 catggcagaa tttcccgcca gcttactgat tcttaatggc aaaagtactg acaatctacc
      361 cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg acgatccatg tgcgggtcac
      421 ctgggagaaa ggcgatgccg cacgatatgt agaggaggcc cggaagttgg gcgtcgcaac
      481 ggtgattgcc ggtggtggcg atggcaccat taatgaagtt tctacggcgt tgattcagtg
      541 tgagggggat gacatacccg cgctgggaat tttgccatta ggaaccgcca atgattttgc
      601 caccagtgta gggattcctg aggcactgga taaggcgctg aaactggcaa ttgccggtaa
      661 cgccattgcg atagatatgg cgcaggtcaa caaacaaacc tgttttatta atatggcgac
      721 aggcggattt gggacgcgta ttaccacaga aacgccggaa aaattaaaag ccgcgctggg
      781 tggcgtctct tacatcattc atggcttaat gcgcatggat actctgcaac cggaccgttg
      841 tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc ctggtcattg gtattggtaa
      901 cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac gcgttaatta acgatggctt
      961 gctgcaactg cgcattttta ccggcgatga aattcttccg gctctcgtat caaccttaaa
     1021 atctgacgaa gataacccga atattatcga aggcgcttcg tcgtggtttg atatacaagc
     1081 cccacacgaa atcactttta atcttgatgg cgaaccgttg agtgggcaaa acttccatat
     1141 tgaaatactt ccggcggcgt tgcgttgtcg attaccacca gattgtccat tattgcgtta
     1201 atcaaactat tctctcttga accagggaga atccgtaatg ccggatgcgg tgtaaacacc
     1261 ttatccggca tacagaacaa tactacaccg taattaattt tacccgtgtc ttctgcaatg
     1321 ccagtttatc cacatctgac acggcatcat cggtaataat aatgtcaatt ttttcgattg
     1381 gcagtacctg attaaaaccg cgacgattaa atttcgatga atcgagaacg gcaatcactt
     1441 tatttgcggc ggtgaccatc gccccgctaa tcgaataacc ttcattaaag gtcgtaatac
     1501 cattaaccgc atcaatacca tcagcaccga caaacattaa atcggcatta atatcctgca
     1561 atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt atggcggacc gtgccaccac
     1621 agacgaccag agtaatgtct ttattttcgg aaagggcaaa cgctgccggg agactgttgg
     1681 taatcaccgt gatatttttg gcggtcatta atccttcggc gataagcatt gtcgtacttc
     1741 cgctgtcgag aataacggtc atcccttcgt ggatcatttt gactgccgcc tgcgctattc
     1801 tgttttttgg cgcgctggca agctgaaatc gctctttaaa cccgacttcc tgggtctcgg
     1861 tttcactatt accagacatt attttcgccg caccgccatg aaagcgcgta accacgcctt
     1921 tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc cgaggcagca aatactcccg
     1981 ccagatcctg aacaagcacg gttccctgtt cattcactaa ttggatgatc ttattccttc
     2041 gctcgaatga attcatcgtc atttacccat attcaattgt ggctagtgta aacgaagggg
     2101 gaccattggt gaatgatctg ttttgcacaa taccgggcca gcatcacgct ggcccgcggt
     2161 ttcagggaat gagcaacact ttgcccggca tagcattacg agcgatgtca cgcaccgcct
     2221 gggcgaagct ttcaaagctt ccacggtgag cgattaatgg ctccaggctt aacttacgtt
     2281 ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg ccaagggctg gagtagttca
     2341 tccaactgcc gataaccgtc agctctttac gcaatatttt gccaaacgtt gccgatgtta
     2401 aatgcagatc ctgatgcaac gtgcccacca gtgccagttg ggcatgagga ccggcaatct
     2461 ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc gaggataagc tgattaaagc
     2521 gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat ttcgctactg ttaaatgttt
     2581 gcatcgcacc gaaagatttt gccagtgcca gtttttctga gctaatgtcg atagccgtca
     2641 cactctttgc tcccagcgcg acagcgcact gaatagccag caggccaatg gttccggcac
     2701 caataataat aacgttttta ttctcacaac cttgcgctaa atgaaaagca tgcaggccaa
     2761 cggtaatcgg ctcaataaaa gccccatcct caataggcat atccgtgggt agagcaaaga
     2821 cattttttcg cttaacgaca atatattcgg caaatccacc atcacgccgt gagccaataa
     2881 aatcatattt tgcgcactgg gaataaaacc ctttcaaaca ctctggacaa gtaaaacagg
     2941 gtaataacgg cacacaggca accgcatcgc cagggtgtaa atcatcaaca ccggatccta
     3001 ccgcatcaat atagccgcta aattcatggc ctaacgttat tggataataa tgtgcaccat
     3061 ttttaaatat cctgggtaaa tcggaaccac ataagcccga gctggcaatt tttacccgca
     3121 cctcatcctg atgtttaatt tcaggaatga cgctttctgc aacgcgcacg ataccatcag
     3181 tatcattcac cactgatttc ataaaaactc ctaattgtta agggggataa cctccccctc
     3241 aggtaaaaat tattctgcga gaacgacttt ctcttgttta ataaagccac gcgctctacg
     3301 ccaggtcatg aaaataccgg taagataaat tgcgccgata ataatgaaac cgggaatatt
     3361 ttgcggggag aaaacctgaa tcagtaacca ggtaatggga gaaccgccct gatccattga
     3421 agccaccata cccccggctt ttaacgcccc agcattagcc gccagttggg tgtgcaaacc
     3481 aatagtttgc gtcgcgatcc acagggtgat gctcataatg atgacacccg agattaaggt
     3541 gcggaacaga tttccacgat gcaccgcgac cgccatcgcc acgaagaagc cgatagtggc
     3601 aagatcgcca aacggcagta cctgattacc aggaacacaa acagcaatta aaatggtgag
     3661 cggaataaaa atcaggcttg ccgataccac cgccgtatgc cccagcagta atgcgggatc
     3721 caggccaatc aggaactcct gaccgccgaa cttcgcctgt aaacgactac gggcctgctt
     3781 agcgatgggc gttaaaccat ccatgatggg tttaatcacc cgtggcatta gcagcatcac
     3841 cgccgccgtt tttaccgcca gctgcaatac acctttgaca tcgtaacccg cgaggatgcc
     3901 gataatcagc cccatcacaa aacccacggt gacaggctcg ccaaacgggc caaatttgcg
     3961 ctgaatatcg tcggcgctaa atttaatacg gttaacgcct gggattttct cgatgatagc
     4021 atcgaccagc accgcaatcg gccccatata cgccgacgta ccgtgcggaa tagcaatgcc
     4081 ttccagctca aagaaatttc gggtatcgcg ggcaaaccag tcgccgagct tataaacaaa
     4141 cgccgcgtga attaccacac ctgccatccc tatcatccat gaaccggttg ccagatgcag
     4201 caacgcgccg gtgaaggtca tatgccagat attccagata tcaacattta ccacccgcgt
     4261 catacgggtc agtaacatcg ccacgttaac cagaatcgca atcggaatcg ccaccagcgc
     4321 aatttgcgac gcccaggtca ttggtgaaga gcccggccag ccgacatcga ccacatgcag
     4381 attcaggtcg aaattttccg ccatcgcttt cgccgccggg ccaatggaat ccagcattaa
     4441 gccaatcaca aggccaatgc caacaaagcc aatcccgata tgcagacccg ctttaaagca
     4501 atcgcctgcc ttcatgccta aaattttaga aaaaataatg atgacaatcg gcaacatcac
     4561 cgtagggccg agatcgagaa tataacgcat gacttctgaa aacataggtc acccctgtaa
     4621 gatagtcaga attttatttt gtaatgcttc gataccgaca ccagaaacaa aaggcatgcc
     4681 gtgaactaac ggaatatcgc caaaactacg atccaccctg gcagtggtgc atatcaaatg
     4741 cacgccatcc atataggttt ctatttcatt aacccgacac tggattaatt caacaggaat
     4801 attatgactc tgacacaact ctttaatttc ttccgccgcc atcgtagagg tcgcaaccgc
     4861 gcctccgcaa gcgacaataa tcttgcgttt cataaatagt cctttttatt gttacagagg
     4921 gatttattcg tctgaatcta aaacatactt tgtgaagtat tcctttaact gagtttcagg
     4981 aagagtgatt agtgtctcaa cgatatcggg ctgttgtaac ttgccaaata aacagcgtaa
     5041 aagtttcaat tgctgctgcg gattttccac aatcaacgca ataaccaacg ataccgccac
     5101 gtcattatca tcatccgctt gctgaaaatg aactttattt gttggcctta acagataaat
     5161 ggctgacgac ttagcatgaa tcgcctcaca atgcggtatt gcaatggcgt gctgctcaag
     5221 cattatcccg gtagggaatt ctgcttctct ggcaattaat gcctgtggcc aggtatcatg
     5281 aaccacacct ttggcgagca tctcattacc gatatgggtt aaaacttcgc tacgatcgac
     5341 aaaagaaatt ccgctacgaa caaacaggtt agtcatatac cgtccttatt ccgcacagcc
     5401 gtagcgatag gcgcgcaaaa catcataaat tttatccata atcagctgat gcggtattgc
     5461 tgataattcc ccggactgaa tgcgttcaaa ttgtttggga agatactgac taatcatgcc
     5521 cagtgggatg tccacgcctt gcagattcac catcatcgtt tcaacgctat ttttaatccg
     5581 actatgcggc cagtaataac gaatacgatc cgacaggctg taacgaatat ccagtaatga
     5641 atcgttaaaa cccgtgcgat aatatttttt ccagtattgc ggttcgtcga gcatcacttc
     5701 ttcaattacc gccaggcaac cgctgcgatt ttcgggggcg ataagttcct gctcaatttg
     5761 cgccagcgca aatatcgcct cacgtaaagc aaaggttaat gcgggaccga ctttcaatat
     5821 tgcaaagtga tcgcggacta attcccaata agccgtccgg gtctggtaat cggtagaatg
     5881 tgcttcataa accattcggg tattttctat ccattgcgcc agcgcctgcg cttcctgcgg
     5941 ctgataatgg ataatattgc tgtgatcaaa ttccacaccc ggctgcacca cgatggcaat
     6001 cacgcgtgtt aatgcctctg tcagcccacg ggcaataaag gccttttgat gcgtacgtaa
     6061 agtattggcg gcatcttcaa catgggtgat gtgtactgac tgaatggcgc tggcctcacc
     6121 gcccggaacc ggaacttcgg tgccaatgac atagctcagt tgctcacgct ggcaatctgt
     6181 cgccacactt tccgcagcaa agcaaagcac agcagctcgt tccgcaaccg tttctggtgc
     6241 taacggtatg ggatcccccg cgcaggacat tgacgcatca agatgaattt tactgaagcc
     6301 agcacgaaca tatgccttta ccagctcgac ggatttttcc atcgccgcat ccacattttc
     6361 ttgctgccag cagtttggcc ccagatggtc accgccgaga ataatacgct cgcgtgcaaa
     6421 tccgacttta tcggcaatcg caaaaacaaa ttcgcgaaag tctgccggtg tcattccggt
     6481 ataaccgcca aattgattga cctggtttga cgttgcttca atcagcactt tgcgcgtgct
     6541 gttgcgatca aatgccagcg ccgcttcgat aaccaacgga tgggcagaac agactgaaca
     6601 tatgccgata tgttcaccag ctttatgccg ggcaattaac gttttcatgt tttttccttg
     6661 ttaaatggca ggtgcgttat gccctgccct cgcagccaca atcggcaatc actttgctca
     6721 ccacatcgcg cattgcggat ttagccgact gcaaataatc ccggggatcg gtcgcttcgg
     6781 ggtgctcggt caggtaattt tttaacgcct gcgagaaggc atttttcagc tccgttgcaa
     6841 cgttgatttt gcatatcccc agtttgatgg tttgctgaat atccttagtc gataaccctg
     6901 acgcgccatg cagcaccagc ggtaagttca cccactggcg aatgttctcc agtcgagaaa
     6961 aatcaagcgc cggtgcgctg gcatacatac catgagccgt gccgatggcg accgccaggg
     7021 aatcaattcc ggttgcctcg gcaaattcac gcgcctgagc ggggttggta taaaaagcat
     7081 cggcttcatt gacttgcaca tcatcttcct ggccgccaag ttgccccagc tccgcttcga
     7141 cgctgacatc aaagcgatgg caaaaatcca ccacctcttt gacccgtgaa atattttgcg
     7201 caaaaggcaa atgcgaggcg tcaatcatga ctgagcgcac gccagaacga accttctgag
     7261 cgatatcgtc aaatttcgtg tgatggtcga gatgaatcgc cagtggatgg tgataatgct
     7321 tcgccatcgc gctgaccagc gccaacagat tttctgtacc agcatgagta aatgtgccag
     7381 gcgttccggc gatgatgacc ggcgcatgca ggttggcagc ggtttctacc accacttgca
     7441 tcgtttcgag attgtgaata ttgaatgccg gaaccgcgta accgccgcgc tgcgcgttgt
     7501 tcagcatctg ctttgtcgat accacgtaca ttttcacatc ctgtcgattg ctttcgattt
     7561 caaaacataa tgaaatcatt tgtttttaaa tgtcgagata acgatcacaa aaacgacaat
     7621 atgaaaatta atagaggaat gaaaggcaaa aaaaacggcc tcccggaggg aagccgcaac
     7681 aaagtgcgcg tggttttatg caggatgcgg cgtaaacata tgtggcagcg ctgcgtaggc
     7741 atgataagac gcttcagcgt cgcatcaggc ataggttgcc ggatgcggcg taaacgcctt
     7801 atccgtccta cggggtagtg ccgttcgtat caggcgatag taattttgct atcgagataa
     7861 acgtcctgca cggcgttaat cagtttcacg ccgtcagcca tcgatttctt gaacgcctta
     7921 cgtccaagaa tcagccccat tccgccagcg cgtttgttga taaccgcagt acgcactgca
     7981 tcactgaggt cagtttcacc gcctgcagca ccaccggagt ttatcaaccc ggcccggccc
     8041 atatagcagt tagctaactg ataacgcacc agatcaatcg ggttttcgct ggttaacttg
     8101 ctgtacacgc gatcgtcggt ataaccgtaa ttaattgctt tatagccgcc gttattttcc
     8161 gccatttttt gtttgacgat atctgcacct atggttgccg ccagatggtt cgcctgacca
     8221 gtcaggtcgg cggagacatg gtaatcaacg ccatctttct taaaggcgga gttacgcaaa
     8281 taggcccaca gcactgtcac catgcccagc tcgtgcgcac gttcaaaagc cgcagaaatt
     8341 tcttcaatct ggcgacgtga ctcttccgag ccaaaataga tagtcgcacc aaccgccacc
     8401 gcgcccatgt tgaaggcctg ctccacgctg gcatacagcg tttgatcgta ggtattcggg
     8461 taacttagcg tctcgttgtg attaagtttt acgagaaatg gaatgcgatg cgcatagcgc
     8521 cgcgataccg acgccaacac gccgtaagtt gatgccacac agttacagcc cgcttcgatc
     8581 gccagttcaa caatgttttt cgggtcaaag tagagcgggt tagcagcaaa tgaagctccg
     8641 gcagagtgct caacgccctg gtcaaccggc agaatagaaa gatatcctgt gccagccaga
     8701 cgcccggtgt tgtacaacgt ctgcatatta cgtaacaccg ctggcgggcg attattgtca
     8761 atcatcacgc ggtctacgta gtcatgtccg gggagataaa gctggtcaga aggaatagtc
     8821 atacaacggt gctgtaaaag gttgtcggcg tctttgccaa gcaactgcgc aatatctgtc
     8881 attactatgc tcccgtaaat tccgattgga tatcggctat ggattgtcct ggcccgcctt
     8941 ttgcgggcaa tcataatcct ggtcgttacg ggcgagtttt tccatctttt ggatgttttt
     9001 tcagcgtttt ctgttggctc gattcatcag aaaaaatgtt agcgcggtca agttttcacc
     9061 gcaaaggtat ttaaaaggta ttattaagtg gtattgtcat cgcgtacctt acattacctg
     9121 tcatgaagga attaaaagat gaaaacaaca acaaagctgt cgttcatgat gtttgttgaa
     9181 tggtttatct ggggcgcgtg gtttgtgcca ttgtggctgt ggttaagtaa aagcggtttt
     9241 agtgccggag aaattggctg gtcgtatgcc tgcacagcca ttgcggcgat cctgtcgcca
     9301 attctggttg gctccatcac tgaccgcttt ttctcggcgc agaaagtgct ggcggtattg
     9361 atgttcactg gtgcggcgct gatgtatttc gctgcgcaac agaccacttt tgccgggttc
     9421 ttcccgttac tgctggccta ctcgctaacc tatatgccga ccattgcgct gactaacagc
     9481 atcgcttttg ccaacgtgcc ggatgtggag cgtgatttcc cgcgcattcg tgtgatgggc
     9541 actatcggct ggattgcctc tggtctggca tgtggtttct tgccgcaaat gctggggtat
     9601 gccgatatct caccgactaa catcccgctg ctgattaccg ccggaagttc tgctctgctc
     9661 ggtgtgtttg cgtttttcct gcccgacacg ccgccaaaaa gcaccggcaa aatggacatt
     9721 aaagtcatgc tcggcctgga tgcgctgatc ctgctgcgcg ataaaaactt cctcgtcttt
     9781 ttcttctgtt cattcctgtt tgcgatgcca ctggcgttct attacatctt tgccaacggt
     9841 tatctgaccg aagttggcat gaaaaacgcc accggctgga tgacgctcgg ccagttctct
     9901 gaaatattct ttatgctggc attgccgttt ttcaccaaac gctttggtat caaaaaggta
     9961 ttattgcttg gtctggtcac cgctgcgatc cgctatggct tctttattta cggtagtgcg
    10021 gatgaatatt tcacctacgc gttactgttc ctcggcattt tgctgcacgg cgtaagttac
    10081 gatttttact acgttaccgc ttacatctat gtcgataaaa aagcccccgt gcatatgcgt
    10141 accgctgcgc agggactgat cacgctctgc tgccagggct tcggcagttt gctcggctat
    10201 cgtcttggcg gtgtgatgat ggaaaagatg ttcgcttatc aggaaccggt aaacggactg
    10261 actttcaact ggtccgggat gtggactttt ggcgcggtga tgattgccat tatcgccgtg
    10321 ctgttcatga tctttttccg cgaatccgac aacgaaatta cggctatcaa ggtcgatgat
    10381 cgcgatattg cgttgacaca aggggaagtt aaatgaaaac agaacgtatt ctcggtgctc
    10441 tttatgggca ggcgttaggg gatgcgatgg ggatgccctc cgagctttgg ccacgcagcc
    10501 gcgttaaagc acactttggc tggattgacc gttttctgcc tggaccaaag gagaacaacg
    10561 cggcctgtta ttttaaccgc gccgaattca ccgacgatac ctcgatggcg ctgtgtctgg
    10621 cggatgcgtt actggaacgt gaaggcaaga tcgacccgaa tctgattggg cgtaatattc
    10681 tcgactgggc gctgcgtttc gacgccttta acaaaaacgt actaggtccg acctcgaaga
    10741 ttgcgcttaa cgccattcgc gacggtaaac ccgttgctga actggaaaac aacggcgtga
    10801 ccaacggcgc agcgatgcgc gtctcgccat taggttgttt gcttccggcg cgtgatgttg
    10861 attcctttat taatgatgtg gcgctggcgt cgagcccgac ccataaatcc gatctggcgg
    10921 ttgcgggcgc ggtagtcatc gcatgggcga tttctcgtgc tattgacgga gaaagctggt
    10981 cagcgattgt tgattcattg ccttcaattg cgcgacatgc acagcaaaaa cgcaccacca
    11041 cgttcagcgc ctcactggca gcgcggctgg agattgcgct gaaaattgtg cgcaatgccg
    11101 acggcactga atccgccagc gaacagcttt accaggtcgt tggcgcaggt accagcacta
    11161 ttgagtccgt tccgtgcgcc attgcgctgg ttgaactggc acaaaccgac ccgaatcgct
    11221 gcgccgtcct gtgcgctaac cttggcggcg atacagacac catcggtgct atggcgacgg
    11281 caatctgcgg cgcgttgcat ggcattaacg ctatcgatcc tgcgttaaag gcggagttgg
    11341 atgcggtaaa tcagcttgat ttcaaccgct atgccacagc gctggcgaaa tatcgtcaac
    11401 aacgggaggc ggcatgagcg gcgctcgatt acacacgctg ctgcctgaat taaccacgcg
    11461 tcagccggtt atggtgattg gcgcggcagt cattgatgtg atcgccgacg cttatgccct
    11521 cccctggcgt gggtgcgata tcgaactgaa acagcagagc gttaacgttg gcggctgcgc
    11581 actgaatatt gccgtggcgt taaagcgcct cggcatcgaa gcgggtaacg ccttgccgct
    11641 cggtcagggc gtgtgggcgg agattattcg caaccggatg gcaaaagagg gcttaatcag
    11701 cctgatcgat aacgccgaag gtgataacgg ctggtgtctg gcgctggttg agccggatgg
    11761 cgaacgcact tttatgtcat tcagcggtgt ggaaaatcag tggaatcgcc agtggctggc
    11821 gcgattaacc gttgcgcctg gcagcctgct ctatttttcc ggttatcaac tggcctcgcc
    11881 ctgcggcgaa ttattagtgg aatggctgga aaagctgcaa gacgtgacgc cgtttatcga
    11941 ttttggccca cgtattggcg atatcccgga ggcattactg gcgcggatca tggcctgtcg
    12001 acctttagtg tcgctcaatc gtcaggaggc tgagattgcc gccgaacgtt ttgctttatc
    12061 cgcagagata acaacacttg gcaagcaatg gcaggagaaa tttgccacgc cgttgatcat
    12121 tcgtctcgat aaagagggcg catggtattt cagcaacgac gcttctggct gcattccggc
    12181 atttcccacg caagttgtag acaccattgg ggcgggcgac agtcatgcag gtggcgtact
    12241 tgccgggttg gcctctggtc tgccactggc ggatgccgta ttactgggca atgcagtggc
    12301 gtcgtgggtt gtcgggcatc ggggcggtga ttgtgcgcca acgcgcgagg aactactcct
    12361 cgcacacaaa aacgtataga tcgctgcgac agtggctaat gctgtactca ataggccgtt
    12421 gctgttggtc aagcgccact tgcttgatca ccagcaccgg tattttgcta tctaactgaa
    12481 tatgtgactg aaactcggca tctggcatcc gggcgctaac gcgggaacgt gtacgttgtg
    12541 ggtaaatgtg ctggctgcgg aaatagtcat aaagagaaat tccgatggca tcaacatcat
    12601 gaattaaatg cgccggaacc cacgattcct caatcgatac cgcttcttca tcgacatagc
    12661 gaatacgctt aagcaaaaag acatcacttc ccgcttcgac cgccagctgc tgcgcgactt
    12721 cttcgggaca tttcacgaca cgcttgttaa cccatagcgt atcgggcttt ttcccgcgca
    12781 ataccacctg ctgagaaaaa cctcgcgctt ctttcaacga gtattcgaag atgttgttga
    12841 tctgcgtgcc gtaaccgcgc gaacgcgtca ccacaccctc ttcttccagc gcctgcatcg
    12901 ccttgcgcac cgtaatgcgt gaaacgccgg ttaactgact taaatcacgc tcaccgggca
    12961 aaatattgcc atgctccagc accccgctgc gcacggcatt ttttaccgtt tcggcaaact
    13021 taatataaag cggcgtgtta tccgccgcta aaatacgctc gttcagttgg gcaatcagct
    13081 gggtatgcgc ttgttccatt tgtctttttc ctgccaggga gtctggtggg agtatatcac
    13141 tgcttcttat ttaaggcgtt tctttaatcc catcaacgaa tgcctgcaac tcctcctctg
    13201 tgccattaaa cacattaaaa tccaccgaac cattaatgcc atctacctgc ccacggtcgg
    13261 aatgctgcca gaaacgccag gccatgccgt cattgtccgg gcgacgttga tagtagtgag
    13321 ccacccacca cggatattca ttgaaatagc ccgccagatt ggtgtgataa aaaacggctc
    13381 ctgagtaaat aaccggcttt tttcccgtac ttttttcgac catttttagc cactgactta
    13441 cccgcttgcg taattctttt gccgataatt ttccccgttc ttctacgtcc agcacggcag
    13501 ggaaatcgcc ttgtgagaaa tccaccgttt gcagaaataa tcgcgcctga acaggcgctg
    13561 ctaccgacgg ggagaaataa tgatacgccc cgcgcagcag gccattttcg cggcttagtt
    13621 gccagttacg cgaaaaatag gggtccacca gcttttcgcc ttccgtcgcc ttgataaagg
    13681 caaattgtaa gcggataccg ttatcgcgca tttttgccac ccgctgccag tcgatccgct
    13741 cctgccagcg cgaaacatct attccgtgaa tggtgtaact ggcaggaata tgaatagcga
    13801 aagatttaac cgggcgataa ccgtagaagt ggatgtagtc tgtaatctga actgctgtcg
    13861 aatagaaaaa attcaccgtc tgacgcggat aaattgcaac gatggaaatt agcccaagag
    13921 cgcataatag agcggtgagt ttttttcgac tggtaattct tagctgcatt ggtttcatcc
    13981 ctgaatgtca gtgccaaagg ctgacaataa ccaaagcagc tatagtacgg tgctcaccac
    14041 caggcgtgga agtggtgaac cggaccaata ccgtgaccaa cttccagcgt gtcggcctgg
    14101 gctaacgccg atgaaagcca gctttttgcc tcctgtacgg tgtcagccca gtttgtatgg
    14161 cgcgggcgta gtgcagccaa cgccgcagag agtgtacaac cagtgccgtg ggtgtttttg
    14221 gtcataatgc gcggtgcggt aaaccgttgt tcaccctcgc gggtaaacag ccagtccggg
    14281 ctttgctcat catccagatg gccacctttc attagcactg cgccacagcc catcgccaac
    14341 agcgctcgcc cttgttccag catttcctgt tcggtgcgcg cgtgtggcgc gtcgagcaag
    14401 gcagcagctt cgggcaagtt tggcgttatt aatgaaacct gtggcaataa tcgactgcgt
    14461 agcgtggcaa ctgccgaagg tgaaagcagt gggtcgccac tttttgccag cataacggtg
    14521 tcgagtacca cgttttggat ctgataacgt tgcaaccgtt ctgccaccgc ttcaacaata
    14581 tcggtttccg ccaacatacc gattttagtg gtatcgattc gcacatcgct gaacaccgaa
    14641 tcgagctggg cggcgacaaa atcaggctca atgcgataca ccgactgtac gccacgggta
    14701 ttttgcgcca ccagtgcagt aataactgag caaccataag cgccaagtgc cgagaaggtt
    14761 ttaagatcgg cctgaatccc cgcaccacca ctcggatcag taccggcaat cgtcagagcg
    14821 ttaattcgtt tcatgcctgc acctcctgcg tcaattgcca gagcgcatca aggaaatgtg
    14881 gaacaaaact gcctggcccc tcgcttctgg cgactgcgcg ttctccggct tgtttcatcc
    14941 agtgacaggc agatgcgaca ttttccagca tatcgcctgg taacgcacaa caggcagcga
    15001 caaccgccga taatgcacag ccagttccta ccactttggt cattaacgga tcgccaccgt
    15061 gaatgccaac ggcgcgatgg ccatcggtaa cataatccac ctcgccagtg accacgacga
    15121 ttgcgccagt ttcccgtgcc agtgtttgtg cagcgggtat cgcgttaact gcggcgtcag
    15181 tggtatccac tccccgtcca ccattagcaa cgccagctaa tgccatgatt tccgaagcat
    15241 taccacgtat cgctgccggt ttaaaagata aaagttcatg acaaaaatgg cggcgataat
    15301 cgagcgcacc aaccgctact ggatcaagcg tccagggtgt ttgagagctt tttgcttgct
    15361 caacggcagc acgcatcgcc tgtgcgcgtg gctgcgtcag tgtgccaacg ttaatcaaca
    15421 aggcactggc gatagccgca aactgactgg cctcttcggt ttcgataacc atcgctggcg
    15481 atgcaccgag cgccagcaag gtattggcag taaaggtttg caccacatcg ttggtcatgc
    15541 agtgcacaag aggggaatgt tggtgaaaaa ggtgtaacgt gtgcgcagat tgcgcggaac
    15601 tcagcaggtc gacttgcata gtttgctcct gccataacgt gaagaagcaa tgacctggtg
    15661 gtccgtgact tccctacgct ggcattatcc agatcaggtg atacgggtat ttctcagcct
    15721 tcacgcagaa gggcaccccg agtcgtttgg ttgaatctct atattttgtg ccagcttagg
    15781 ggaagaggct tgcagatgca agctcgcctc cattcattac gtcttcactg tgtaccccat
    15841 atcagtaatg aaaaagaaac aattcacacc catcattcag gtggtaaaaa ctgtagacta
    15901 tcttaattag ctaatttata ttatttatct aaatttgata ggtgagataa aaatgacaga
    15961 aacccagatc tacgagaaca ttaagcaagc cattagcagc gcaccacgaa atagccaaac
    16021 gatggaaatg cacctccaga tgatcaaata cgctgaccat ctgaaaaacg tcacggcaaa
    16081 agagttttgc gaaggtgtag gacttaaagc cagctttgct acagagttca gcaaaatgag
    16141 aaatcttacc gagcgactca aagccgccgg acttgatacc acgaagttat aacaaacgaa
    16201 atcatcaata tattactttc gttttccagg gttccctgta tatgtaccta tgatcataaa
    16261 aaccagttac caccacgtag ctgataccat aatattttca tttttacttt aaataaatag
    16321 ataaatttag agagtggaat aattaaggtc tattttctct agctttttga gattgatagt
    16381 tgagaatgat aattctttca tctcaataac accgaagttt gatagctgtt taagccacag
    16441 ttgaagacca gcatcagagc aacctgctcg atatgttaca tcactacatt ttattagtgt
    16501 tgatttcctc ttatagagat aaataaggac ttcatagatg cgttttccgg tgtcattgga
    16561 tacgcgctat acccccattc ttggctaaat cttggttcat aatcatactt taattaaaat
    16621 ggatctaaaa attcatcata gatggctgga tttttcatag aaaaatatgt ttgaacacca
    16681 tttgatattt ttatttattg atcacaggct aacgttcttg aatattctat aaatgatgct
    16741 tcctctattc taactctacc cacgcaatat atatacaacc ctacctatac tcgaagtacg
    16801 ttcaacacat aagacaacga aaagtagttg tgtgctgtaa aatttaaata ttttaataat
    16861 taatttcaag agcatgctgt gccccctata ttatgcaaat gaaaagtagg aaaaaatcat
    16921 aagacacatt aaacgattac aataatcaaa cctatattaa ggtgaataga aggtattcga
    16981 atgagtcatt tctaatattt ttagtcttac gggcaaaggc tatctattac ctaacatgca
    17041 atggaaagcg gaaaagtatg tagatagtgc aacttgttcc ctgaactgct gtttatccaa
    17101 cctattgcta tgaaaatacg taat
//
LOCUS       DAZKIF010000102        16934 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZKIF010000102 DAZKIF010000000
VERSION     DAZKIF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.102"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            72..590
                     /gene="yfiR"
                     /locus_tag="ACHOTW_003424"
     CDS             72..590
                     /gene="yfiR"
                     /locus_tag="ACHOTW_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP9845469.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            580..1806
                     /gene="dgcN"
                     /locus_tag="ACHOTW_003425"
     CDS             580..1806
                     /gene="dgcN"
                     /locus_tag="ACHOTW_003425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP9845470.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1822..2304
                     /gene="yfiB"
                     /locus_tag="ACHOTW_003426"
     CDS             1822..2304
                     /gene="yfiB"
                     /locus_tag="ACHOTW_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9845471.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITPP
                     "
     gene            complement(2381..2728)
                     /gene="rplS"
                     /locus_tag="ACHOTW_003427"
     CDS             complement(2381..2728)
                     /gene="rplS"
                     /locus_tag="ACHOTW_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9845472.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2770..3537)
                     /gene="trmD"
                     /locus_tag="ACHOTW_003428"
     CDS             complement(2770..3537)
                     /gene="trmD"
                     /locus_tag="ACHOTW_003428"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9845473.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3568..4116)
                     /gene="rimM"
                     /locus_tag="ACHOTW_003429"
     CDS             complement(3568..4116)
                     /gene="rimM"
                     /locus_tag="ACHOTW_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9845474.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4135..4383)
                     /gene="rpsP"
                     /locus_tag="ACHOTW_003430"
     CDS             complement(4135..4383)
                     /gene="rpsP"
                     /locus_tag="ACHOTW_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9845475.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4520..5881)
                     /gene="ffh"
                     /locus_tag="ACHOTW_003431"
     CDS             complement(4520..5881)
                     /gene="ffh"
                     /locus_tag="ACHOTW_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9845476.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6048..6839
                     /gene="ypjD"
                     /locus_tag="ACHOTW_003432"
     CDS             6048..6839
                     /gene="ypjD"
                     /locus_tag="ACHOTW_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP9845477.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6861..8147
                     /gene="yfjD"
                     /locus_tag="ACHOTW_003433"
     CDS             6861..8147
                     /gene="yfjD"
                     /locus_tag="ACHOTW_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP9845478.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVPDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVVGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8202..8795)
                     /gene="grpE"
                     /locus_tag="ACHOTW_003434"
     CDS             complement(8202..8795)
                     /gene="grpE"
                     /locus_tag="ACHOTW_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9845479.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8918..9796
                     /gene="nadK"
                     /locus_tag="ACHOTW_003435"
     CDS             8918..9796
                     /gene="nadK"
                     /locus_tag="ACHOTW_003435"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP9845480.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9882..11543
                     /gene="recN"
                     /locus_tag="ACHOTW_003436"
     CDS             9882..11543
                     /gene="recN"
                     /locus_tag="ACHOTW_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9845481.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11692..12033
                     /gene="bamE"
                     /locus_tag="ACHOTW_003437"
     CDS             11692..12033
                     /gene="bamE"
                     /locus_tag="ACHOTW_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP9845482.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12095..12385)
                     /gene="yfjF"
                     /locus_tag="ACHOTW_003438"
     CDS             complement(12095..12385)
                     /gene="yfjF"
                     /locus_tag="ACHOTW_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP9845483.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12375..12812)
                     /gene="ratA"
                     /locus_tag="ACHOTW_003439"
     CDS             complement(12375..12812)
                     /gene="ratA"
                     /locus_tag="ACHOTW_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP9845484.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            12983..13465
                     /gene="smpB"
                     /locus_tag="ACHOTW_003440"
     CDS             12983..13465
                     /gene="smpB"
                     /locus_tag="ACHOTW_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9845485.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13680..14042
                     /gene="ssrA"
                     /locus_tag="ACHOTW_003441"
     tmRNA           13680..14042
                     /gene="ssrA"
                     /locus_tag="ACHOTW_003441"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14311..14559
                     /locus_tag="ACHOTW_003442"
     CDS             14311..14559
                     /locus_tag="ACHOTW_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9845486.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
     gene            complement(14675..14827)
                     /locus_tag="ACHOTW_003443"
                     /pseudo
     CDS             complement(14675..14827)
                     /locus_tag="ACHOTW_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(15061..15651)
                     /gene="espM2"
                     /locus_tag="ACHOTW_003444"
     CDS             complement(15061..15651)
                     /gene="espM2"
                     /locus_tag="ACHOTW_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFP9845487.1"
                     /translation="MPMNTTGMSFSSFGISCHRENSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSGNILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFFLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            15834..16484
                     /locus_tag="ACHOTW_003445"
     CDS             15834..16484
                     /locus_tag="ACHOTW_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9845488.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            16563..>16934
                     /gene="espW"
                     /locus_tag="ACHOTW_003446"
     CDS             16563..>16934
                     /gene="espW"
                     /locus_tag="ACHOTW_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFP9845489.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNPVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDID
                     SLFLKKLNNVRNQNKFPIDVKE"
BASE COUNT         4424 a         4333 c         3849 g         4328 t
ORIGIN      
        1 ataatgatat tttaatgagc attctgaaaa aatatcgatg catttcgagc gaagatggtg
       61 gggatccctg aatgcgtttt tctcaccgac tgttcttact gctgattctc ctgctgacgg
      121 gagcaccaat actcgctcag gagccttctg acgttgcgaa aaacgtgcgt atgatggttt
      181 ccggtattgt cagttacacc cgctggcctg cgctatcagg gccaccaaag ttatgtatct
      241 tttcatcttc acgttttagt accgccctac aagagaatgc cgcaacatct ttgccttatc
      301 tgcctgtcat cattcacact caacaagagg cgatgatttc aggctgcaat ggtttttatt
      361 ttggcaacga gtcaccgaca ttccagatgg aattaaccga acaatatccg tcaaaggcgt
      421 tattattaat tgccgaacaa aataccgagt gcattattgg tagcgccttt tgtctgatca
      481 tccacaataa tgacgtcaga tttgccgtaa acctggatgc cttatcgcgt agcggtgtaa
      541 aggtcaaccc ggatgtctta atgctcgcac ggaagaaaaa tgatggataa cgataattct
      601 cttaataagc gccccacgtt taaaagagca ttacgcaaca tcagtatgac cagcatattt
      661 atcactatga tgctgatctg gttgctgctt tccgtgacct cggtgctgcc cctgaaacag
      721 tacgcgcaaa aaaacctggc actgacagca gcaacaatga cttacagtct ggaagcagct
      781 gtcgtttttg ccgatggccc tgcagcaact gaaacactgg cagcgctggg ccagcaaggg
      841 caattttcaa ctgcagaagt acgtgataag cagcaaaata ttctggcatc ctggcattac
      901 acccgtaagg atccaggcga tactttcagc aatttcataa gccactggct cttccctgcc
      961 cccatcattc agccgattcg tcacaatggt gaaaccattg gcgaagtacg cttaaccgct
     1021 cgcgacagtt caatcagcca ttttatctgg ttttcgctcg ccgtactgac cggttgtatt
     1081 ctgctggcat caggaatcgc aattaccctc acccgccatt tgcacaatgg cctggtggaa
     1141 gcactgaaaa atatcaccga tgtcgtacat gatgtgcgtt ccaaccgcaa tttttcccga
     1201 cgagtttcgg aagaacgtat cgctgagttt caccgcttcg ctctcgactt caacagtctg
     1261 ctggatgaaa tggaagagtg gcagcttcgt ttacaggcca aaaatgcgca gcttctacgt
     1321 accgcgctac atgacccatt aaccgggctg gctaaccgcg cagcgtttcg tagcggcatc
     1381 aatacgttga tgaacaattc cgctgcccga aaaacgtcgg cgttactatt tcttgatggc
     1441 gataatttca aatatatcaa tgatacctgg ggtcatgcga cgggcgatag agtcttgatt
     1501 gaaatcgcaa aacggttagc tgaatttggc gggctgcgac ataaagcata ccgcctgggc
     1561 ggcgatgaat tcgctatggt gctctatggt gtacagtcgg aatctgaagt gcagcagata
     1621 tgctcagcac tgacacaaat ctttaatctc ccgtttgatc ttcataatgg ccatcagacc
     1681 accatgacat taagcattgg ttacgcaatg accattgcgc acgcttctgc ggaaaaatta
     1741 caagagctgg ccgatcacaa tatgtatcag gccaaacacc agcgtgccga aaagctggtg
     1801 agataacaag gatatatcga tatgataaag cacctggtag cacccctgat tttcacctca
     1861 ctaatactga ctggctgcca gtcccctcag ggaaagttta ctcctgagca agtcgccgct
     1921 atgcaatctt atggatttac tgaatccgcc ggcgactggt cgctgggctt atcagatgcc
     1981 attctgttcg caaaaaatga ctacaaattg ctcccggaaa gccagcaaca gatccaaacc
     2041 atggcagcca aattggcctc gacagggcta acacatgccc gtatggatgg acacaccgat
     2101 aactatggtg aagacagtta caacgaaggc ttatcattga aacgggcgaa tgtcgtggcc
     2161 gatgcatggg ctatgggtgg acaaattcca cgcagcaatc tcaccacaca gggtttagga
     2221 aaaaaatatc ccatagccag taacaagacc gcccagggcc gcgccgagaa ccgccgcgtc
     2281 gcagtggtga ttactccccc ttaaaacacg agcaaacaga taaaaaaagg gccagccaat
     2341 tggccagccc ttcttaacag gatgtcgctt aagcgaaatc ttagttaaga cgctctttga
     2401 tacgagcagc cttaccagta cgctcacgca ggtagtacag tttagcttta cgaacagcac
     2461 cacgacgttt gacagaaatg ctgtcaacta ccggagagtg agtctggaag acacgctcaa
     2521 cgccttcgcc gttggaaatt ttacgaacag tgaatgcaga gtgcagaccg cggttacgaa
     2581 tagcgataac cacgccctcg aatgcctgca gacgtttttt ggaaccttca acaacccata
     2641 ctttcacttc cacggtatca cccggacgga aggaaggtac gtcctgcttc atctgctctt
     2701 gttcaagttg cttaataatg ttgctcataa tttaatctct tatcctgggt aaactgatat
     2761 ctcgggggct tacgccatcc catcatgttt atgttgctgt tgtgcgtgtt ccgttttgaa
     2821 ctccgccagc aaccttgctt gctcttcagt cagagccagg ttttccagaa gttcaggtct
     2881 tctaagccag gtacggccca gcgactgttt caaacgccag cgacgtatct cggcatggtt
     2941 gcccgacagt aacactggcg gaacttccat cccttctaac acctcaggcc gcgtatagtg
     3001 cgggcaatcc agcaatcctt cagcaaagga atcttccgtt gccgaggctt catgtcccag
     3061 tactcccgga ataaaccggg aaacggagtc aatcagcgtc attgctggta actcaccacc
     3121 actgagaacg taatcgccga ttgaccattc ttcgtcaatt tcggtttgga tcacgcgctc
     3181 atctatacct tcgtagcgac cgcacaccag aatcaatttt tgattcgttg ccagttcgct
     3241 gacgcccgct tgatcaagct tgcgtccctg tggtgaaaga taaatcacct ttgcgccttc
     3301 acccgccgcg gcttttgctg catgaatggc gtcccgcaag ggttgcacca tcattaacat
     3361 ccccggtccg ccgccgtaag gacgatcgtc cacggtacgg tgccggtcat gcgtgaaatc
     3421 gcgaggactc cagctctgga tgctcagcag gccattttta actgcccggc cagttacccc
     3481 gtaatcggta attgcgcgga acatttcagg aaacaggcta attatgccaa tccacatagc
     3541 gccgtctttt accgtttatc cggtggttta aaaaccagga tcccaatcta cttcgattga
     3601 acgagtagtg agatcgactt tcttgataac ctgcccatcg aggaacggta cgagacgttc
     3661 cttgatacca aacgcatctt tcaggtttgc cttaatgacg agaacgtcat tggatccggt
     3721 ttccatcata tcgacgactt taccgagatc gtagccttca gtggttacta cctggcagcc
     3781 catcaggtct ttccagtagt agtcaccctc ttcaagctga ggcagctgcg atgaatccac
     3841 gacaatttca caattcgtca gcaggttcgc cgcatcacga tcgtcaacgc ctttcagctt
     3901 gatgatcatg tcctgattgt ggtgcttcca gctttccagc tggacttgct gccactgacc
     3961 cgccttctgg ataaaccagg gctgatagtc aaaaatgctt tcggcgtctt cggtggaaga
     4021 aaacactctg agccacccac gaataccgta agacgaaccc atttttccca aaacgatggg
     4081 atcaacaggt gcttgcgcgg tgagttgttt gctcatcatg accaccgtga cagattaagc
     4141 tgctttgttt acttctttga tcagcgcagc aacgcgatca gaaatagttg cgccctggcc
     4201 aacccagtga gcgatgcgat ccagatccag gcgagtgcct tcttcttttt cgctagcgat
     4261 tgggttgaag aaaccaacgc gctcgatgaa gcgaccgttg cgtgcattac ggctgtcagc
     4321 gacaacaacc tggtagaacg gacgcttttt agcgccgtga cgtgctaaac gaatagttac
     4381 cataacatcc tcttgtgtga ataaaacaac cggaccccat cgaggaacgg agtccggtgt
     4441 catattaaaa gcccgaaaat tttactgatt tctggagaaa atgcaatcag cagttaataa
     4501 ctctgtcgca taaggtcatt tagcgaccag gaaatcccgg cggcatcatc cccttcatgc
     4561 ttctcatcat cttcgccatt ccgcccttct tcattttctt catcatgcgc tgcatgtcgt
     4621 cgaactgttt cagaagacgg ttaacgtcct gcacctgcat accgcaaccg gcagcaatac
     4681 ggcgtttacg cgaacctttg atgatttctg gcttagcgcg ctctttcatc gtcatcgagt
     4741 tgatgatggc ttccatacgc accagcactt tatcgtccat ctgtgacttg acgttatccg
     4801 ggatctgccc catgcccggc agtttgccca taaggctggc catgccgccc atatttttca
     4861 tctggcgcag ttgctcaagg aagtcgttga gatcgaagcc gtcacctttt ttcagcttgc
     4921 tggctaattt ctctgcctgc gcgcggtcaa ctttgctttc gatatcttcg atcagcgaca
     4981 gtacgtcgcc catgccgaga atacgcgacg cgatgcggtc cggatggaac ggctccagcg
     5041 cctcagtttt ctcgccaaca ccgaggaact tgatcggttt gccagtgatg tgacgaatag
     5101 agagcgccgc accgccgcgg gcatcgccgt ccactttggt caacactacg ccggtaagcg
     5161 gtaacgcttc attgaatgct tttgccgtat tggccgcatc ctgaccggtc atggcgtcaa
     5221 ccacaaacag ggtttcaacc gggttaatcg acgcatggac ttgtttgatc tcgtccatca
     5281 tcgcttcgtc aacgtgcaga cgaccagcgg tatccaccag cagcacgtcg tagaatttca
     5341 gtttggcttc tttcagcgcc gcgttaacaa tatcaaccgg cttctgacca acatcagaag
     5401 ggaagaaatc aacgcccacc tgctctgcca gcgtctcaag ctgtttgatt gccgccgggc
     5461 gatagacgtc agcagaaacc accagcactt tcttcttgtg cttctcgcgc aggaacttac
     5521 cgagtttacc aacgctggtt gttttaccgg caccttgcag gcctgccatc agcacgaccg
     5581 caggcggttg cgcagccaga ttcagggtct ggttctcttc gcccatcgcc gcaaccagtt
     5641 cgttacggac gattttgacg aactcctgcc ccggcgtcag gctcttatta acttcatgac
     5701 caaccgcttt ctcttttacg cgattgataa actcacgcac taccggcaga gcgacgtccg
     5761 cctccagcag cgccatgcgc acttcgcgca gcgtatcttt tacgttgtct tcagtgaggc
     5821 gtccacggcc actgatattg cgcagcgtgc gcgacaaacg atcggttaaa ttatcaaaca
     5881 ttgtctctcg cctggggtgg aaacggttgg ccgcaatcgc gacacatcat cagtattttg
     5941 ccgcagtata acatgaaggc gtctttgttg ttatgcaacg gttggagcag cgttcacctg
     6001 acgctatact gcttctcttt cttattgctc aaactgtcga catcactatg cccgtttttg
     6061 ctctgctcgc gcttgtcgcc tactccgtca gtcttgcgct gattgttccc ggtctgctgc
     6121 aaaaaaacgg cggctggcgg cgcatggcta ttatttctgc ggtcattgcg ctggtctgcc
     6181 acgcaatcgc tctggaagcc cgcatcctgc ccgacggtga tagcggacaa aacctcagcc
     6241 tgctgaacgt tggttcattg gtcagtttga tgatctgtac ggtaatgacc attgtggctt
     6301 ctcgcaatcg tggctggttg ctgctaccca ttgtctatgc ctttgcgctt atcaacctgg
     6361 cgctggcaac cttcatgccc aatgaataca tcacccatct ggaagctacg cctgggatgc
     6421 tggtgcacat tggcttatcg ctcttttcct atgccacgct aattatcgcc gccctgtacg
     6481 cgctgcaact ggcgtggatt gattaccaac tgaagaacaa gaagctggcg tttaaccagg
     6541 aaatgccgcc attgatgagt atcgagcgta aaatgttcca catcacgcag attggcgtgg
     6601 tgctgctaac gctcacgctt tgcactggcc tgttctacat gcacaactta tttagcatgg
     6661 aaaatatcga caaggctgtg ctctctatcg tggcgtggtt tgtctatatt gtgctgctat
     6721 ggggacatta tcatgaagga tggcgtggac gtcgcgtcgt ctggtttaac gttgcgggtg
     6781 cagtcattct gacactggcc tacttcggca gccgaattgt ccagcagtta atcagctaaa
     6841 cccagaaaag gagtttcccc ctggaacaca tttctactac tacgttgatc attattctga
     6901 tcatcatggt ggtcatttca gcctattttt ccgggtccga aaccggaatg atgaccctca
     6961 accgctatcg tctgcgacat atggcgaaac agggtaatcg ctcggccaaa cgcgtcgaaa
     7021 aattgctgcg taagccagac cgcctgataa gcctggtgtt aatcggcaat aacctggtca
     7081 atattcttgc ctccgcgctc ggcactattg ttgggatgcg tttgtacggc gatgcgggcg
     7141 tggcaattgc gactggtgtg ctgacttttg tcgtactggt ttttgctgag gtattgccga
     7201 aaaccattgc cgcgctgtac ccggaaaaag tcgcttatcc gagtagtttt ctgctggctc
     7261 ccctgcaaat tttgatgatg ccgctggtct ggttgctgaa tgctatcacc cgtatgctga
     7321 tgcgcatgat gggtatcaaa accgatatcg tggttagcgg ctctttgagc aaagaagagt
     7381 tgcgcactat cgtgcacgaa tcgcgctcac aaatttcccg tcgcaatcag gatatgctgc
     7441 tgtcggtgcc cgatctggaa aaaatgaccg ttgatgacat catggtgccg cgcagtgaaa
     7501 ttatcggtat tgatatcaac gatgactgga aatctattct gcgccaactc tcccactcac
     7561 ctcacgggcg catcgtgctc taccgtgatt cgctggacga cgccatcagt atgctgcgag
     7621 tacgtgaagc ttggcggttg atgtcggaga aaaaagagtt caccaaagaa accatgctgc
     7681 gcgccgcgga cgagatctat tttgtgccgg aaggtacgcc gctcagcacg cagttggtga
     7741 agtttcagcg caacaaaaag aaagtcggcc tggtcgtcaa cgagtatgga gacattcagg
     7801 ggctggtgac ggttgaagat attctggaag agattgtcgg cgatttcact acgtcgatgt
     7861 cgccaacact tgccgaagaa gtcacgccgc aaaacgacgg ttcggtgatt attgatggca
     7921 ctgccaacgt gcgggaaatc aacaaagcct ttaactggca tctaccggaa gatgatgccc
     7981 gcacggttaa tggcgtcatt cttgaggcac tggaagagat ccctgtcgta ggcacccgcg
     8041 tgcgtattgg cgagtacgat atcgatattc tcgacgtaca ggacaatatg attaagcagg
     8101 taaaagtttt tcctgtgaaa ccgctgcgcg agagtgtggc ggagtaacga aaacggcccg
     8161 gcattcgcat gcagggccgt gaattattac gaaaacagaa attacgcttt tgctttcgct
     8221 acagttacca tcgccgcacg aatcgtacga ccattcagcg tataaccctt ctgcataatg
     8281 cccagtacgt tacctggcgc aacgtcatca gattccacca ttgcgatagc ctgatgcaca
     8341 ttcgggtcca gcggcacgtt agtttcggcg atcacttcaa caccaaactt acgcacaaca
     8401 tccagcatcg acttcagcgt cagctcaatg ccttcaacca tcgcagacat atccgggtta
     8461 gctttatcag ccacttccag cgcacgatcc aggctatcaa tcaccggcag caattcgttg
     8521 atgaatttct ccagcgcgaa tttgtgggct ttttcaatat ccagttcagt acgacgacgc
     8581 aggttttcca tttcggcttt tacacgcaaa atgccgtcac gttcacgggt ctgggcttca
     8641 gccagctgag cttcgagatt cgcaattttt tcatcgcgcg gatccacctg ctcagcagaa
     8701 gcttctggct caactgcctc aatctcttcg tgctgatcca tgataatttc ttccggggct
     8761 tgcccctcag gcgttttctg ttctttacta ctcatgaatt tctccgcgtt tttttcgcat
     8821 tcatctcgct aacttcgctt attatgggga tcagtttcag ggtttcaagg gaagcactca
     8881 cattgtcatc aatcttcgca acaaggacct cggaaaaatg aataatcatt tcaagtgtat
     8941 tggcattgtg ggacacccac ggcaccctac tgcactgaca acacatgaaa tgctctaccg
     9001 ctggctgtgc acaaaaggtt acgaggtcat cgttgagcaa caaatcgctc acgaactgca
     9061 actgaagaat gtgaaaactg gcacgctcgc ggagattggg caactggctg atctcgcggt
     9121 agtcgttggt ggcgacggta atatgctggg cgcgacgcgc acgctcgccc gttacgatat
     9181 taaagttatt ggaatcaacc gtggcaacct gggtttcctg actgaccttg accccgataa
     9241 cgcccagcaa caattagccg atgtgctgga aggtcactac atcagcgaga aacgtttttt
     9301 gctggaagcg caagtctgtc agcaagattg ccaaaagcgc atcagcaccg cgataaatga
     9361 agtggtactt catccaggca aagtggcgca tatgatagag ttcgaagtgt atatcgacga
     9421 gatctttgcg ttttctcagc gatctgatgg gctaattatt tcgacgccaa caggctccac
     9481 cgcctattcc ctctctgcgg gcggtcctat tctgaccccc tctctggatg cgattaccct
     9541 ggtgcccatg ttcccgcata cgttgtcagc acgaccactg gtcataaaca gcagcagcac
     9601 gatccgtctg cgtttttcgc atcgccgtaa cgacctggaa atcagttgcg acagccagat
     9661 agcactaccg attcaggaag gtgaagatgt cctgattcgt cgctgtgatt accatctgaa
     9721 tctgattcat ccgaaagatt acagttattt caacacatta agcaccaagc tcggctggtc
     9781 aaaaaaatta ttctaatttt acgccagcct ctttactgta tataaaacca gtttatactg
     9841 tacacaataa cagtaatggt ttttcataca ggaaaacgac tatgttggca caactgacca
     9901 tcagcaactt tgctatcgtt cgtgagcttg agattgattt tcatagcggc atgaccgtaa
     9961 taactggcga aaccggtgcg ggtaaatcta ttgcaataga tgccctcggt ctttgtctcg
    10021 gtggtcgcgc tgaagccgac atggtgcgta ccggcgctgc tcgcgctgac ctgtgcgccc
    10081 gtttttctct gaaagatacg ccagcggccc tgcgctggct ggaagaaaac cagcttgaag
    10141 acgggcatga atgtttgctt cgtcgcgtga tcagcagcga tggtcgctcc cgtggtttca
    10201 tcaacggtac agctgttcct ctgtcacaac tgcgcgaact gggtcagttg ctgattcaga
    10261 tccatggtca gcacgctcat cagttactca ccaaacccga gcaccaaaaa ttcctgcttg
    10321 atggctatgc caatgaaacc tctctactcc aggaaatgac cgcacgttat cagttgtggc
    10381 atcaaagctg ccgtgacctc gcgcatcatc aacagttaag tcaggaacgc gctgcccgtg
    10441 cggaactgct gcaataccaa ttaaaagaac ttaacgaatt taatccgcag cccggagagt
    10501 ttgagcaaat cgacgaagag tacaaacgtc tggcgaacag cggtcaattg ctgaccacca
    10561 gccagaatgc attggcatta atggccgacg gtgaagacgc aaacctgcaa agtcagcttt
    10621 acacggctaa acaactggtg agcgaattga ttggcatgga cagcaaactg tccggcgtac
    10681 ttgatatgct ggaagaagct accatccaga ttgttgaagc cagcgacgaa ctgcgccact
    10741 actgcgatcg tctggatctc gatcccaacc gactgtttga acttgaacag cgcatctcaa
    10801 aacagatttc gctggcacgt aaacatcacg tcagccctga gacattgcca cagtattacc
    10861 agtcgctact ggaagaacag cagcaactgg acgatcaggc cgactcacaa gaaacgctcg
    10921 cactggcggt aacgaaacat catcagcagg cactggaaac ggcgcgcgca ttacaccagc
    10981 aacgccagca ttatgcagaa gaacttgcac agctgatcac cgacagtatg catgcgctct
    11041 caatgccgca tgggcagttt acgattgatg tcaaatttga cgagcatcac ctgggcgctg
    11101 acggcgccga tcgtattgag tttcgggtaa ccaccaaccc aggtcagcca atgcagccta
    11161 ttgccaaagt cgcatccggt ggtgaattgt cccgcatcgc attggcaatt caggtcatca
    11221 cggcgcgtaa aatggaaacc ccggcactga tttttgatga agtggatgta gggattagcg
    11281 gtccaacagc ggcagttgtc ggcaaactgc tgcgtcaact cggcgaatca actcaggtga
    11341 tgtgtgttac ccacctgcca caagtcgcgg gatgtggtca tcaacactat tttgtcagca
    11401 aagaaaccga tggtgcgatg acagaaacgc atatgcaatc cctgaataaa aaaacgcggt
    11461 tacaagagct ggcgcgcctg cttggtggca gtgaagtcac acgtaacaca ctggcgaatg
    11521 cgaaagaact gcttgcggcg taaacttttt tcctgcttca cggtcagagt aaacagcaaa
    11581 acgccgtaag accggaaagc aaaaggtttt aaagtgatga aaggtctatt atcatcggca
    11641 tattacagat gagccacgta ctgctcgggc ccgaaaagga atcaaatcac tatgcgctgt
    11701 aaaacgctga ctgctgcagc agcagtacta ttgatgttga ccgcaggctg ttccactctg
    11761 gagcgagtgg tttaccgtcc tgacatcaac caggggaact atctgaccgc taacgacgta
    11821 tccaaaatac gtgttggcat gacgcaacaa caagttgcgt acgcattggg tacaccgctg
    11881 atgtccgatc catttggtac gaatacctgg ttctatgtct tccgccagca accaggtcat
    11941 gaaggtgtaa cgcagcaaac gctgacgcta acctttaaca gtagcggtgt gttgaccaat
    12001 attgataaca aacctgcgct gagtggtaac taataataaa gttgctctca aagacgttaa
    12061 aaaaggtgct caatgagcac cttttttctg tccgttattt attcgctgat ttttctgctc
    12121 gttgcctgcg aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc
    12181 catcatgcac actatcgctt aattttgccg gacggctgta aatgccgact ttgttttcag
    12241 ttaaatcgat atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg
    12301 tcgcgccctc ctgcagcgtc acgcgctgca ggtactgctt cttaggtagc gcataagcca
    12361 cctcaacggc aattttacct ggcactgtaa acctctttcg cacgaaccgt aaaagcctgg
    12421 accatattag acgccagctc tttaaacacg cgaccaaaag cgagttcaat caacttattg
    12481 gtaaactcaa agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac
    12541 ttccatccac caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg
    12601 ttactggtca actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct
    12661 accgccgcag tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc
    12721 aaaaactgag gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag
    12781 ggtaccagtg cggtccggct aatctgaggc ataacaattt ccatcaacaa aaatccaaca
    12841 aataatatca tttatcctgc agtaaaaaaa acgctatccc ggcgctgggt aacatccggt
    12901 tcatgctaag atagagcctt gtcccccgca ggattgatat ggggtatttt cgatttcaga
    12961 ttaccgatga ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac
    13021 catcgcgctt aacaagcgcg cccgtcacga atactttatt gaagaagagt tcgaagcggg
    13081 acttgccctg caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atattagcga
    13141 cagctatgtc cttctgcgcg acggagaagc gttcctgttt ggcgcaaaca tcacgccaat
    13201 ggctgtggct tctacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa
    13261 ccagcgcgaa cttgattcgt tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc
    13321 gctctccctg tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa
    13381 gaaacagcac gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcgcg
    13441 tatcatgaaa aacgcccacc gttaaacctg cactccaatt cttgacctgt tcctcaccgc
    13501 gcctccctct ccggcggcgc gaatgaacat cttattggct atcacatccg acaaaaatgt
    13561 tgccatccca ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga
    13621 taacgccatt gaggctggtc atagcgctca taaatctggt atacttgccg ttacacattg
    13681 gggctgattc tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg
    13741 gcctcgtaaa aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta
    13801 ataacctgct tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg
    13861 tcaaacccaa aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc
    13921 aggctagttt gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta
    13981 agcatgtagt accgaggacg taggaatttc ggacgcgggt tcaactcccg ccagctccac
    14041 cactttaaga aggactacaa ccggacagta gcaataaata cagccactta cggacacaag
    14101 ccagacagta gtcggactga aaaaagacaa aaatatacac gtgaaatgca cgtgcacttc
    14161 taaaaaaccc cagttatcac cgactggggt ttttcaattt gtaactaagg gtaacaaccc
    14221 ccccgttttt ttcatctcac tcaccttgat actgtttatt tgtacagtta aaaataatac
    14281 tgtatacaaa cacagtatag agggactttt atgcgtattg agatctgcat agccaaagaa
    14341 aaaatgacta aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc
    14401 atcagtaaac gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt
    14461 tctgttacgc gcaccgccga taaagattca gctaaaactt ttgttcagga gactctgaaa
    14521 gatacctgga agtctgctga cgagtggttt gttcactaat taacacgtaa aatcggtaac
    14581 ggctggaaat cattcaatac tcgcactatc gaaagttcac cagccaaccg cagcacgtcc
    14641 tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc
    14701 actcaatatc aggtgcagtt gatgtatcaa cacggttcag caacacccga tactttttct
    14761 aggcttccag caatgaggtt tcttcctccg ttgcaatttc cagatctgca gcatcctgaa
    14821 gtggggcgca ccacgcaaac atcacaaaat attgtcgctg cgatgaacct gtacaataaa
    14881 taatgaatca cggtacgttt ctgtgtataa cgtactgtag ttttctttac gatttatagc
    14941 tgtactggtg agccatgagt aatctgaatc catgcatggc gtgtggtgcc gttgtgcatt
    15001 ttctgcgtct cttttactgg tccgaagccg atgatgctga agcagtcctc tcctctagcc
    15061 tcatccctgt atagcacgca tcaaataatc tgggatatca tttcgtagtt ttacaatatc
    15121 aatatccgaa tcgatatgtt tctggtaaaa cttgtccgca agcaagtttg ctatggtatc
    15181 tgatgtacga ttttttacat cccggctcat accatcacaa agagcatcca ttttcttgcc
    15241 aaaagaagca ctccccatta ttacatgatt aatagaactt tgtgctgcct taacatcaag
    15301 ggttagttga ttctcctggc tcacaagaaa aaagactctt tgcctctctt cagtggaaat
    15361 ttttgcattt ttttgaaaac agtaattatc aatctcctgg ttgatcaccc ttgagggata
    15421 tactatccgt tcatctttta tccactcatc aatatcttta gtactctgcc ttctcaccgt
    15481 ttccagtata ttgccgctaa atttgcgaac agaaaagcta atagtccgtt ctccaattga
    15541 acattttacg acctcatcat tcttccctcg gaaagagttc ctgaaggagt tctctctatg
    15601 gcaacttata ccaaaagagc tgaaagacat acctgtagta ttcatcggca ttataattcc
    15661 tttttataaa aaagtgttta caatattgca tacttgcgtt tattgtagcc agaaaaaaca
    15721 tgcatgcaat aatattctat taaaaattgt attgttttat tcacatatca tgagcggcat
    15781 gttgattctt gttaccaata tagttcacaa tatggattaa aaaggataat aatatgccag
    15841 tcatattaaa tttttctaat ggaagtgtat tgccggaaaa tgagctggag gctttacgac
    15901 atatagcaag aagcaaccag aatgacacta ttactatagg aggccgtaat atgaggcttc
    15961 attatatcca gtttatggat ggttttagtg ttgagcctat tcttggtgga ctttgggacc
    16021 atcttggggc aagagaggct catcatctcg cagaccgcct gacaagacaa cttaacggag
    16081 gtaacacttt tctgcaggca tacagcttat atctggagca gaggcaagct gccccgcttg
    16141 tgcaggagag cgtcataaaa acactactag atcgaataaa ttcgaatgca tttcccgtta
    16201 gtttacaaga cttttcctgt actgaggaac atcttaattg tccgataacg ctacatattc
    16261 ctgagacagg tgtttttgtc agaaatgctc gaaattcaga aatatgtgca ttatatgatc
    16321 aggaagcatt gactgaactt atcctgcgta acgctctcca ccccctcagc cgtgacccct
    16381 ttgctccaga aatgattata agcaaagaca agtgtcattt taatataaca aaacaatgtt
    16441 tttacgcatt acccatatac ccacttcaac aaaacagtat ttaaaaataa aagcacaata
    16501 taagaagcat attttcaata agttaatcac catataacaa acatatagcg aggagactat
    16561 ttatgcccaa aatatcatca gttgtatcat catgttacca tctgttcagt gaacatcaac
    16621 aactttcaaa tgaaacaaca atgacgaacc ccgtctccag aagaattgtt cataaagaat
    16681 atggtatatc tttaaaatcc gttcctgtat ggttggctac agctaaaact ccccttgctc
    16741 tactcaatgg cagacataca agaagtcact catttattat tgcagggacc ccaggaatgg
    16801 gaagcaggag cggagcccaa tactatgcca taaacagtga tgataaacgc tcccgaatag
    16861 acattgactc tttattttta aaaaagttaa ataatgtgcg aaatcaaaat aaatttccaa
    16921 tcgatgtaaa agaa
//
LOCUS       DAZKIF010000103        16874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZKIF010000103 DAZKIF010000000
VERSION     DAZKIF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.103"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            66..2762
                     /gene="stcE"
                     /locus_tag="ACHOTW_003447"
     CDS             66..2762
                     /gene="stcE"
                     /locus_tag="ACHOTW_003447"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFP9845490.1"
                     /translation="MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYF
                     NTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTP
                     VQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVN
                     KLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTV
                     FYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLV
                     IKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSR
                     MIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLN
                     STAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGH
                     NYGLGHYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPF
                     DGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADT
                     QEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASA
                     DNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPE
                     QFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQL
                     RFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQ
                     ALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDN
                     LSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
     gene            2849..3724
                     /gene="gspC"
                     /locus_tag="ACHOTW_003448"
     CDS             2849..3724
                     /gene="gspC"
                     /locus_tag="ACHOTW_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP9845491.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            3725..5692
                     /gene="etpD"
                     /locus_tag="ACHOTW_003449"
     CDS             3725..5692
                     /gene="etpD"
                     /locus_tag="ACHOTW_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFP9845492.1"
                     /translation="MFRKWLNGRLPVLVFTTVILGAIPGWGAEFSANFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKET
                     DKTAIPAWMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTR
                     QNEILDSDQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTL
                     NEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            5692..7197
                     /gene="gspE"
                     /locus_tag="ACHOTW_003450"
     CDS             5692..7197
                     /gene="gspE"
                     /locus_tag="ACHOTW_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP9845493.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            7199..8422
                     /gene="gspF"
                     /locus_tag="ACHOTW_003451"
     CDS             7199..8422
                     /gene="gspF"
                     /locus_tag="ACHOTW_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP9845494.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            8453..8887
                     /gene="gspG"
                     /locus_tag="ACHOTW_003452"
     CDS             8453..8887
                     /gene="gspG"
                     /locus_tag="ACHOTW_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP9845495.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            8884..9438
                     /gene="gspH"
                     /locus_tag="ACHOTW_003453"
     CDS             8884..9438
                     /gene="gspH"
                     /locus_tag="ACHOTW_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP9845496.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            9435..9800
                     /gene="gspI"
                     /locus_tag="ACHOTW_003454"
     CDS             9435..9800
                     /gene="gspI"
                     /locus_tag="ACHOTW_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP9845497.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            9797..10396
                     /gene="gspJ"
                     /locus_tag="ACHOTW_003455"
     CDS             9797..10396
                     /gene="gspJ"
                     /locus_tag="ACHOTW_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP9845498.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            10393..11370
                     /gene="gspK"
                     /locus_tag="ACHOTW_003456"
     CDS             10393..11370
                     /gene="gspK"
                     /locus_tag="ACHOTW_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP9845499.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            11304..12581
                     /gene="gspL"
                     /locus_tag="ACHOTW_003457"
     CDS             11304..12581
                     /gene="gspL"
                     /locus_tag="ACHOTW_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP9845500.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            12568..13080
                     /gene="gspM"
                     /locus_tag="ACHOTW_003458"
     CDS             12568..13080
                     /gene="gspM"
                     /locus_tag="ACHOTW_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFP9845501.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            13201..13971
                     /locus_tag="ACHOTW_003459"
     CDS             13201..13971
                     /locus_tag="ACHOTW_003459"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9845502.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            14063..14464
                     /gene="eptO"
                     /locus_tag="ACHOTW_003460"
                     /gene_synonym="gspS"
     CDS             14063..14464
                     /gene="eptO"
                     /locus_tag="ACHOTW_003460"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFP9845503.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            14643..15814
                     /locus_tag="ACHOTW_003461"
     CDS             join(14643..14892,14892..15814)
                     /locus_tag="ACHOTW_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9845504.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
     gene            16355..16870
                     /gene="ehxC"
                     /locus_tag="ACHOTW_003462"
                     /gene_synonym="hlyC"
     CDS             16355..16870
                     /gene="ehxC"
                     /locus_tag="ACHOTW_003462"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFP9845505.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
BASE COUNT         4214 a         3664 c         4744 g         4252 t
ORIGIN      
        1 caacgactag tgaataagtc gctcctgaaa aaataaaata tagaaatact gttatatccg
       61 gctgcatgaa cactaaaatg aatgagagat ggagaacacc gatgaaatta aagtatctgt
      121 catgtacgat ccttgcccct ctggcgattg gggtattttc tgcaacagct gctgataata
      181 attcagccat ttatttcaat acctcccagc ctataaatga tctgcagggt tcgttggccg
      241 cagaggtgaa atttgcacaa agccagattt tacccgccca tcctaaagaa ggggatagtc
      301 aaccacatct gaccagcctg cggaaaagtc tgctgcttgt ccgtccggtg aaagctgatg
      361 ataaaacacc tgttcaggtg gaagcccgcg atgataataa taaaattctc ggtacgttaa
      421 ccctttatcc tccttcatca ctaccggata caatctacca tctggatggt gttccggaag
      481 gtggtatcga tttcacacct cataatggaa cgaaaaagat cattaatacg gtggctgaag
      541 taaacaaact cagtgatgcc agcgggagtt ctattcatag ccatctaaca aataatgcac
      601 tggtggagat ccatactgca aatggtcgtt gggtaagaga catttatctg ccgcagggac
      661 ccgaccttga aggtaagatg gttcgctttg tttcgtctgc aggctatagt tcaacggttt
      721 tttatggtga tcgaaaagtc acactctcgg tgggtaacac tcttctgttc aaatatgtaa
      781 atggtcagtg gttccgctcc ggtgaactgg agaataatcg aatcacttat gctcagcata
      841 tttggagtgc tgaactgcct gcgcactgga tcgtgcctgg tttaaacttg gtgattaaac
      901 agggcaatct gagcggtcgc ctaaatgata tcaagattgg agcaccgggt gagctgttgt
      961 tgcatacaat tgatatcggg atgttgacca ctccccggga tcgctttgat tttgccaaag
     1021 acaaagaagc acatagggaa tatttccaga ccattcctgt aagtcgtatg attgttaata
     1081 attatgcgcc tctacaccta aaggaagtta tgttaccaac cggagagtta ttgacagata
     1141 tggatccagg aaatggtggg tggcatagtg gtacaatgcg tcaaagaata ggtaaagaat
     1201 tggtttcgca tggcattgat aatgctaact atggtttaaa tagtaccgca ggcttagggg
     1261 agaatagtca tccatatgta gttgcgcaat tagcggcaca taatagccgc ggtaattatg
     1321 ctaatggcat ccaggttcat ggtggctccg gaggtggggg aattgttact ttagattcca
     1381 cattggggaa tgagttcagt catgaagttg gtcataatta tggtcttggt cattatgtag
     1441 atggtttcaa gggttctgta catcgtagtg cagaaaataa caactcaact tggggatggg
     1501 atggtgataa aaaacggttt attcctaact tttatccgtc tcaaacaaat gaaaagagtt
     1561 gtctgaataa tcagtgtcaa gaaccgtttg atggacacaa atttggtttt gacgccatgg
     1621 cgggaggcag ccctttctct gctgcaaacc gtttcacaat gtatactccg aattcatcgg
     1681 ctatcatcca gcgttttttt gaaaataaag ctgtgttcga tagccgttcc tccaccggct
     1741 tcagcaagtg gaatgcagat acgcaggaaa tggaaccgta tgaacacacc attgaccgtg
     1801 cggagcagat tacggcttca gtcaatgagc taagtgaaag caaaatggct gagctgatgg
     1861 cagagtacgc tgtcgtcaaa gtgcatatgt ggaacggtaa ctggacaaga aacatctata
     1921 tccctacagc ctccgcagat aatagaggca gtatcctgac catcaaccat gaggccggtt
     1981 ataatagtta tctgtttata aatggtgacg aaaaggtcgt ttcccagggg tataaaaaga
     2041 gctttgtttc cgatggtcag ttctggaaag aacgtgatgt ggttgatact cgtgaagcgc
     2101 gtaagccaga gcagtttggt gttcctgtga cgaccctggt ggggtattac gatccggaag
     2161 gcacgctgtc aagctacatc tatcctgcga tgtatggtgc ctatggcttc acttattccg
     2221 atgatagtca gaatctatcc gataacgact gccagctgca ggtggatacg aaagaagggc
     2281 agttgcgatt cagactggct aatcaccggg ctaacaacac tgtaatgaat aagttccata
     2341 ttaacgtgcc aacagaaagt cagcccacac aggccacatt ggtttgcaat aacaagatac
     2401 tggataccaa atcgctcaca cctgcgccag aaggacttac ctatactgta aatgggcagg
     2461 cacttccagc aaaagaaaac gagggatgca tcgtgtccgt gaattcaggt aaacgttact
     2521 gtttgccggt tggtcaacgg tcaggatata gccttcctga ctggattgtt gggcaggaag
     2581 tctatgtcga cagcggggct aaagcgaaag tgctgctttc tgactgggat aacctgtcct
     2641 ataacaggat tggtgagttt gtaggtaatg tgaacccagc tgatatgaaa aaagttaaag
     2701 cctggaacgg acagtatttg gacttcagta aacctaggtc aatgagggtt gtatataaat
     2761 aatcctgaga cggtgcggcc acttcggccg cattagtaac acattcgtcc cgttaatcaa
     2821 aacataaagt agcgtccagc gttttattat gttgttcttt ctatcattcc ggggtgaccg
     2881 gggcttattt ataaaagata tcgtacttaa aatgctgacg ccaaaccggc tattgtgcgt
     2941 aattttactt attgccggat atcagctggt gtcggttatc catcatttct ggctgactca
     3001 ggcagcatcg gtgcccggct tatcccgtgt tagcgcgccg gaaacagcgg taactggtga
     3061 tcaaactgaa gaacgttttg tttttacgtt attcggcagg gcatccccac tatcatcgga
     3121 ggggagagcg caggaaacaa tgccttccct gtcagatgat ctgctttcag gggaggatct
     3181 tgacgtgaga ggtatacttt atagttcggt tgcagagcat tccgttgcca tatttgcaca
     3241 taataacaga cagttcagtc tgagcgtcgg cgaaaaagta cccagctacg atgctacgat
     3301 tagtgctatt tttagcgatc atattgttat taactatcag ggaaaaactg tatcactgcc
     3361 tctgcgatat gataataccg aaaaaaagaa tgcatatgac aataataatt taacagttgg
     3421 agacgtgata acccaagata attttcgggt agaaagtgtt tttgatatta tgagcttttc
     3481 agccgttacg gttaataata cattaagcgg ttatcgcttg attccgggta agcacagttc
     3541 gttattttat aatgctgggt tgcatgataa cgatctggcc gtatcggtta atggttcaga
     3601 attgcgtgat accagacagg cgcagcagat aatgaagcaa ttgccagaac ttaaagaaat
     3661 aaaaataacc gtcgagcgtg atggtcagtt atatgatgca tttattgctg taggagaaaa
     3721 ctgattgttc aggaaatggt tgaatgggcg tttgccggta cttgtgttca ctacagtaat
     3781 tttgggggcc attccagggt ggggggctga attttcggcc aactttaaag atacggatat
     3841 tcaggagttc ataaatactg tcagtaaaaa tttacacaaa acggtaataa ttaatcctga
     3901 cgtgcaggga accatcactg tacgcagcta cgatatgctg aacgaggaac aatattatca
     3961 gttctttctc agtgtgctgg acgtttatgg ttttgctgtg gtcgatatgc acaacggtat
     4021 actgaaagta gtgcgctcaa aagatgccaa aacgtcggcg gtgccggtag ctagtgatgt
     4081 cagtcccggg actggtgatg aggttgttac ccgggtggtc cccgtaagta acgtggcagc
     4141 cagagatctg gcgcctttgc tgcgtcagct caatgataat gctggcgcag gaagcgtggt
     4201 gcattatgaa ccttctaatg ttttgttgat gaccggacgt gctgcagtga tgaaacggtt
     4261 gatggagatt gttgaacgtg tggataaggt gggtaatcgc agcgttgcca cggtcccgct
     4321 cacctacgcg tccgcaacag acgtagccag acttgttacg gaactgacta aagaaacaga
     4381 taagacagct atacctgctt ggatgacggc gaaactggtt gcagacgaga ggacaaactc
     4441 agtgctcgtc agcggagagc caatctccca acagcgtatc atctccataa ttaagcaact
     4501 ggatcgtcag gaggatgttc agggtaatac taaggtgatt tacctgaaat atgcgaaggc
     4561 gaaggattta gtggaagtcc tgacaggtat cagcagcagt attgaaaacg actctaaaaa
     4621 gagtccgtca acggaagcct tgcgcaaagg agtgacgatt aaatcccacg aacaaaccaa
     4681 tgccctgatc ctgacggggg cccctgacgt catccgcgac cttgaaaatg tgatttcgca
     4741 gttggatatt cgtcgtcctc aggtcctggt ggaggccatc attgctgaaa tacaggatgc
     4801 tgacgggctg aaccttggga tccagtgggt gaataaacat gccggtgtgg cgcagttcac
     4861 cagtaccggt ttacctatta ccacgatggt tcagactcgt cagaacgaaa tcttagacag
     4921 cgatcagagc aatgccctga gcatgtttaa cggaattgca gcggggtttt atcagggaaa
     4981 ctgggcgatg ctgttgacgg cgctctccac aagtagcaag aatgatatct tggcgacccc
     5041 cagtattgtc acgctggaca atatggaggc cactttcaat gttggtcagg aggtcccggt
     5101 actttcgggc tcacagacaa cctctgggga caatattttt aacacggtcg agcgcaaaac
     5161 ggtggggatc aaactcaggg taaaacccca gatcaacgag ggtgattccg tgttactgga
     5221 gatagaacag gaggtgtccg gtgtggcgga cactgcagta gccaccacta ctgacttggg
     5281 agcaaccttc aacacccgaa cagtgaccaa tgccatgctg gtcgggaatg gcgaaacggt
     5341 ggtggtcgga ggattactgg ataagtcgat cagggggagt gagagtaaag tgccactgct
     5401 gggggatatc ccggtactgg ggcatctttt tcgcgcaaaa agcgaacaga cagctaagcg
     5461 taatctgatg ctgttcattc ggccaactat tattcgtgag cgcgacggat ttcgtcatgc
     5521 ttcggccgaa aaataccagt cgtttaatca ggaacaggtg cagtcgcgtg gcaaagaaac
     5581 aacggcgctg acgctgaatg aggaacagct caggctgtcc cccgatcaag acgatacggc
     5641 tttccggaag gtgaaagcgg cgattgctgc gttttatgcg caggagatgt aatgagcaga
     5701 gtagtacaga atgtcagtga atcacgtccc cttctgcctt tttccttttc ccgtacgcag
     5761 agaattttgt tgctgcgtga acaggagggg aatcgagtgt tctgcatgga ggacacacct
     5821 gccagtgccc tgttggaggt gcgcagggtg gctgaaggtc ctctgaatgt cacaacagtg
     5881 tcggcggaag cgtttgaaaa acagctggtg agcagctatc aacgtgattc tgatgaagcg
     5941 cgacaaatga tggctgagat tggtaatgaa atggattttt ataccgtcgc aggggagttg
     6001 cctgaccgtg aggacttact ggatgcgaat gacgatgcgc caattatccg cctgattaac
     6061 gcaatgctga cagaggcaat taaagagaaa gcctcagata ttcatattga aacttacgaa
     6121 cgccatctgc aggttcgctt tcgcattgat ggcgttctgc gggagatcct ccgtttacat
     6181 aggaatctgg cttcgttact gatttcacgc attaaagtca tggcccggtt ggatattgcc
     6241 gaaaaacgcg ttccccaaga tggccgcatg gtactgcgta tcggtggtcg ggcggtggat
     6301 gtgcgtgttt caacgttgcc ttcaaatcat ggtgaacgca tcgtgctgcg tttactagac
     6361 aaaaatagcg ttagtctcga tcttgctgca ctcggcatgt cgcagcagaa tcagcgacac
     6421 attgatgcac tgatccgccg tcctcacggc attattttgg tgaccgggcc tacagggtcg
     6481 ggtaaaagca cgacgcttta tgccgcgtta agcctgctga atccccgaga ccgcaacatt
     6541 atgacggtcg aggatcctgt tgaatatgaa ctggacggta tcagtcagac ccaggtcaac
     6601 ccgaaggtgg acatgacctt tgcccggagt ctgcgcgcta ttttacgtca ggatccggac
     6661 gtggtgcttg tgggggagat ccgtgacggt gaaacagccc agattgctgt gcaggcctcg
     6721 ctgacagggc atttggtgct gtcaacgtta catacgaata gtgcagcagg cgcgctgtcg
     6781 cgtctgcagg atatgggcat ccctcctttt ctgctttcca cctcattact ggctgttctg
     6841 gcccagcgac tggttcgcac gttgtgtccg cgctgtcgtc agccctgtca ggtcagtact
     6901 gagttagcga tggacatgga cattccccct gaaaccacga tctggcagcc tgccgggtgt
     6961 cagcactgca gtttcacggg ctatcacggg cgcaccggga tccacgagct gttgctgatt
     7021 gacgatcgca ttcggacggc aatctatcag ggggaggggg agctgggtat tacccgcttg
     7081 gcagggagtc gctatctgac gctgcgtggc gacgggcgac aaaaggtact agccggtgag
     7141 accagttggg aggaggtggt tcgcgttact gaaagcagat tgcaggaaga ggaatgatat
     7201 ggcgttgttt cattatcagg catcagatat tcacggtaga aaacgcagtg gcattctgga
     7261 ggcggattcg gcccgacatg cccgtcagtt gctgcgtgag caggcgctta ttccagtcag
     7321 gttggatgag aaacaggtcc atcacaagca ctcactgcgg agtatcctga ggtttcgtcc
     7381 gcgcgggggg agcagcgccg aactcgcgtt actgacacgg cagttagcca cactagtggc
     7441 cgcatctctg ccgcttgaag aggcgctgga tgcgttgttg aggcagagtg aaaaaccgcg
     7501 tcagcggaac ttaatcgctg ccgtgcgcac caaggttctc gagggacatt ccttggccgc
     7561 ggcgatgggc atgtttccag gtacgtttga gcgtctttat tgtgcgatgg tggcggcggg
     7621 ggaaacatcc ggtcgtttgg acgtggtgct cagtcgtctg gctgactata ccgagcagcg
     7681 ccagatcatg cgaaaccgtc tactccaggc attgctctat ccttgtgttc tgacgttggt
     7741 ggcggttggg gttattgcca ttctgcttac tgcggttgtg ccgaaagtgg ttgagcagtt
     7801 tattcatatg aaacagaccc ttcccttatc tacccgcgta ctgatggggg ctgctgaggt
     7861 gagccagact tggggcccgt ggctgttact ggccgcagcg ctgggcggga tagcggggcg
     7921 catgatatta catcagccct cccagcgtct ggcttttcat catctgctgt tgcggctgcc
     7981 ggtcgtgggg cgcatatcgc gcggtctgaa taccgcacgt tatgcccgca cgctgagcat
     8041 tcttaatgcc agtgcggtac cgctgctcca ggcgatgcac atcagtggtg acgtactgag
     8101 taatgactgg gcccgtcatc agttagctac cgcggccgag ttggttaggg aaggggtcag
     8161 cctgcatcag gcactggagc agacttcgct gtttccgcct atgatgcggc acatgattgc
     8221 cagcggtgaa aatagtggcg aactcgacag catgttggaa cgggccgccg acaatcagga
     8281 tcgtgagttc agcacacaga tgcaactggc gctgggattg tttgaaccgc tgttggtggt
     8341 gggtatggcc ggggtcgttt tgtttattgt tctggcaatt ctgcagccgc tcctgcagct
     8401 caacaacatg atgaatatgt gaatccttct ttaacagtca acggagaaaa acatgcgaaa
     8461 acagcatcag cgtggtttta ccttgctgga aataatggtg gtaattgtga tcttgggagt
     8521 tttggccagt ctggtggtcc cgaatctgat gggaaataag gataaggccg atcggcagaa
     8581 agtgatgagc gacttggttg cgctggaaag cacgctcgat atgtaccggt tagataataa
     8641 ccggtatccg acaacggagc agggactgcg ggcactggtc agtaaaccca ccgtgcaacc
     8701 tgaaccccgt aactaccggc aggatggtta tatccgccgt ttgccccagg atccttgggg
     8761 gggggattat cagctgttaa atcctggtca gtacagcgac attgatatct tttctcctgg
     8821 cccggatggt gtgccaaaca ctgaagatga tattggtaac tggacgctcg gtaacgcgca
     8881 gccatgagtc agcgcggttt cacaatactg gagatgatgc tgatcctttt gttgatgggc
     8941 atcactgcag ggctggtact aatgtcattt cctgactctg ctcagaatca tctgcaacaa
     9001 cagcgggagc gtctgcaggc gcagctggat tatgccttgg atagaagcca gcaggacggc
     9061 ttgctgatgg ggattcaggt tcggtttgac ggatggaaat ttaaggttct gcagcgcgga
     9121 acagcggaaa gccccccgac attagctgag ggaggggata tttggcaggg gtatgtctgg
     9181 caaacttggc aaccgcgacg tgcagccatg gggggcaaac ttccggatga tgtgcgtctt
     9241 gagctgcagt atctcagagg tttgcagtgg atgtctgagc atgatgacgg ggctgaaccc
     9301 gatattttgc tgcttcccgg aggtgaggta acgccgttca gacttctttt caggcaggtc
     9361 ggagaggagg ccgttgtcgg gctgcaggtt gatgagaacg gtttgatgac tctttttgaa
     9421 ggtgaggtct cattgtgaag gggcaagaag gaatgacatt actggaagtc atagtagctc
     9481 tggttgtgtt tgcgctggca ggaatggcgc tgatgcaggc ctcgacccag caggctgcgg
     9541 ggatcggacg gatggaagag aaggtgctgg caggctggtt ggccgataac cagatggtgc
     9601 aattgcaact cgagaaaact tggccggaaa atggctgggg cgagaagacg atcagcttcg
     9661 ctggcaccga atggtattta cgctggggcg ggccggacag cgacgtgccg cagccgcgct
     9721 ccttagaggt agaggtacgg cgcactaaag aggagacgtc cgccttggtt tccttgcgta
     9781 gcagtgtggt tcgtgaatga gtcagcaacg ggtaaaaggt tttacgctac tggaaatgct
     9841 gttggcactg gctgtttttg cagcgttgag catcagcgca tttcaggtgc tgcagggtgg
     9901 aatacgggcg cacgagctgt cccgggataa agtacagcgt ctggctgagc tgcagagagg
     9961 gatcagccag atggagcgag atcttacgca gatgctccca aggcacagcc gggggaatga
    10021 aggcctgctg ctcgctgcac ctcatctgct aaaaagtgat gactggggga tttcttttac
    10081 gcgaaacagc tggctgaatc ccgccgggat gctgccgcgc ccggagttac agtgggtagg
    10141 ttatcggttg cgacagcaaa agttagaacg cctgagctac tttcatgtcg atcacccttc
    10201 cggtgtttct ccggatgtca gggtgatgct ggacggcgtt cacgcatttc gtctgcgttt
    10261 ttttgttaac ggtgattggc aggcgcgctg ggacagtacc ggcatcctgc cgcaggctgt
    10321 tgaagtgacg ctggttatgg atgattttgc tgaactgccc cgtctgtttc ttgtgagcaa
    10381 ggagaccgct gaatgaaatt gcgggaacag ggcgtggcgc ttctggtggt attgctgatc
    10441 ctgtcgttaa tggtaacgat agcggcggtg attgcggagc gcaacggtcg gacatttttg
    10501 cgtacagtag cgcagttgga tcagctgcag gcaaagtggg atggctatac ggcagagacg
    10561 atagcaaaac aaatcttaca gaggagcagg caggagtcgc cccggaaaac gcatctggca
    10621 caaaattggg cgcagtcaga acgacagttt gagaccagag ggggagatgt gcgggggcag
    10681 atcgtggatg cgcaggcctg ttttaatctt aatgctatta attatggagt ggtggatctg
    10741 accagtatac cttatgccgc cagaattttt cagcagctat taatcaactt gcaggtagag
    10801 cttttacagg cccggcaagt gacggcagcc ctacgtgact ggatcgatcg tgatgataag
    10861 ccggtaaggg gcggtgctga agacgaggtg tatatgggga tggaacctcc ctatctggca
    10921 gccaaccagc cgatgcagga cgtcagtgag ttacgcctta tccggggaat agatgccagg
    10981 ctttaccgca aacttttgcc gtatgtttgt gtactgccta cgtcagatct gtctgtgaat
    11041 gtgaatactc tgctggattc acaggcgccg ctgctggcgg cgctctttct gacgaaaccg
    11101 gacagtcttc cggtgacgga attactgcag cggcgacccc gtactggttg ggagagcgtg
    11161 gcggcgtttc ttgacgcgcc accgcttaag gacattgaca ccagtgcggc catgccggta
    11221 ttggccgtca gcagcaacta ttttctggtt cggttacatg tgcgctccgg ggaacacctg
    11281 ttcagtcagc agactttaat gcagtggagg gaggagcgtt tccggatcat tcagcgacag
    11341 tatggattaa cgatgaggga ggttccctga aaaaaaataa caaacatgtc gaggctggtc
    11401 ggctttttct gcgactgccg gaggatgact cggagccttt attgtggagg ctgattgcgc
    11461 cagaggagac aggagccggc agtgtggcag tcggagaaac ggatccgcag ttagtcggtt
    11521 tgctgtcccg ttatcccgcc tgggtgctgg ttccgtcagg cagaattgcc ttccatcagg
    11581 tcacgctgcc acaccgcacg tggcgtcagc gtttgcaggt gctgcccttt cttctggaag
    11641 agcagttggc gacagacatt gagcagctgc acttcgccat tcttcatcag tcgggtgaca
    11701 ggtgtgatgt ggcggtggtc gaaaaagcgg ttatgcatcg ctggttagcg tactgtggac
    11761 aacttggggt tcgggaggtg aggttgctgc ctgacgtact gatgctgccg ttggcaactg
    11821 aaggatggag tgccgtaaag cttgccgggc agtggctgtt tcgccgggat cgttacgccg
    11881 ggatgacggt agagttatcg tggctgacac atttcttgtc tcttactgcg ccgccggtga
    11941 ttgagtccta ctccgtgccg ccagtaccgt gcccgggtcc gaagaccact gagtggcgaa
    12001 atcagcccgg acgcgatctg ctgcagttgg tggccgaagg tgacgggtat gatggcgcag
    12061 atctgcggca gggagaattt gcccgccagg gaacatggta tgcacgattt cgcccatgga
    12121 ggtatgtcgc aggggctctg ctggcctgtg tttttctggc cgggagcaac gcggggctgg
    12181 cacattatcg gctctggcag caggctcaat tctggcggta tgagagcgta cgtgtctatc
    12241 agcagctgtt tccgagtgaa aaggcggtga aggatccccg ccggcagatg ctgagacatc
    12301 tgcaacagtc gacgggtaac aatataccgg aactcgggag cgtgatgcgc cagcttcagc
    12361 aactgctgtc agaaacctca acgatacgct tacaggcact cgcctgggac agcagcagcc
    12421 agacactgaa aattgatttg caggcggctt cttttcaggc gctggaacac tttcagcaga
    12481 tcgccggaga aaaatacctc atccagcctg gtgagatacg acagcagccg cagggggtag
    12541 aaagtcgtct gatcctgaga gtgaacaatg aacgagctta aaaaacgttt gcagtatgtt
    12601 agcccccgtg agcgcaggct gcttatcgga tgtgttgcct tactggttgt tgtttttgtc
    12661 tattacgtcc tctggcagcc ctggctgata cgagaggata agtggcggac tgtagttacc
    12721 cgtgaaaaaa acacagtgga gtggatgaaa caacaggtac ccaacataaa gagacgggag
    12781 gcaagtatgc ctgaacaggg ggagccgtta ggtttatctg cggccgtgac acgtacttca
    12841 gccgcatacg gtgtatcggt cacccggttg cagcctcaag gggagcgtct ggccgtcacc
    12901 ttagccccgg ttgaatttaa tgcgctgatg cagtggctta cgcaactgga gcgtcggcac
    12961 cgtattcgta ccatggtatt tgatgtggcc gcgcagggca acccacccgg acaggtaacg
    13021 gttaatcggc tggttttgtc ccaagatgac gtaaacaagg acgcttcgct tagtcgctga
    13081 tgtcgtaatt tacagattaa tgcttgatgt aaaaattgga cttataaagg aggatgtgtg
    13141 agtagtttgc cggaatttgc ggttgattat cccttgttgt ggctcatctg caccggaggg
    13201 ctggggggcg ttaccggcag ttttcttaac gtcgtgattc atcgtctgcc gataatgttg
    13261 gagaggcggt ggcggcgtga agcgctgacg ctattggcac attctgtgcc tgagtccggg
    13321 ccggccttta acctgatgat accgcagtct cattgtccct gctgttttca tccgctcggc
    13381 atcagggata acattcccct gttaagctac ctatttctga aggggaaatc acgctgttgt
    13441 ggcgagcgta ttcccgttta ctacccgcta gtcgaaataa cgaactcagt gttattcatt
    13501 ctggccgcga gcaggtttcc accagggctg acattagcgg ctgcctggct ttttatttcc
    13561 atgctgttgg ttctggcggt tattgactgc catacggcgc tgctgccgga tgtgctgacg
    13621 ttgccattgc tctggcttgg cctgttattc agtttacaga gaggcgttgt caccttagag
    13681 gaggcggtag tgggggcggt atctgggtat ctctgtctgt ggggactgta ctggcttttt
    13741 cgtttcgcta ccgggaggga agggttaggt tacggtgatt ttaagctagc tgcggcattg
    13801 ggagcatgga tgggttggca ggcattaccc tcaatacttt tgtttgcctc agtcagtggg
    13861 ttaatcgtaa cagtactgtt gcgtattgta acagctacgg actttacccg cccgctgcct
    13921 tttgggccgt ggctcgcttt gagcggagtg tgccattttt tgttgatgta gcgttcggtt
    13981 accggggcta gtaataaaaa cggctcagcg gataaaagaa ctgttgtaaa ccggtatcag
    14041 gttaaacgat acctagtaaa aaatgatggg taatattttg aaaaaactga attgtatcgc
    14101 gtcattacta gtcttagtga ctatctctgg ttgtcatcag tcaccctcca tccataagca
    14161 ggccactgtt cctccgagcg aacaactgga acaaatggcg tcgattgtct ctgcgacacg
    14221 gtatctgaaa atgagatgta accgtagtga tttaccagat gaacagtcga tcctaaatgt
    14281 cgctaaccgg atcgctatcg ggaaagggtg gcagtcatta actcaggagg atatacgtaa
    14341 acacagcgat gatatctatg tgcgtctgac tagggacagt acgcctgagt atataaaatg
    14401 cagagaattc aatcggcggc tggtcccatt tatcggtgaa ttattggcaa ggggtcgtgg
    14461 ataatatgaa ccgccccgga aatcctggag actaaactcc ctgagaaaga ggtaaacagc
    14521 atgaataaaa atgggtcttc ccttgtagtg gtggctgaag gcatgatgat tgtagtggca
    14581 cactgaattt ggccacctga acagaggtga tatgctcacc tcagaacaac acaggtgctc
    14641 caatgaaaaa aagaaatttc agcgcagagt ttaaacgcga atccgctcaa ctggttgttg
    14701 accagaacta cacggtggca gatgccgcca aagctatgga tatcggcctt tccacaatga
    14761 caagatgggt caaacaactg cgtgatgagc gtcagggcaa aacaccaaaa gcctctccga
    14821 taacaccaga acaaatcgaa atacgtgagc tgaggaaaaa gctacaacgc attgaaatgg
    14881 agaatgaaat attaaaaaag gctacaaccg cgctcttgat gtcagactcc ctgaacagtt
    14941 ctcgataatc gggaaactca gagcgcatta tcctgtggtc acactctgcc atgtgttcgg
    15001 ggttcatcgc agcagctaca gatactggaa aaaccgtcct gaaaaaccag acggcagacg
    15061 ggctgtatta cgcagtcagg tacttgagtt gcataacatc agccatggtt ctgccggggc
    15121 aagaagcatc gccacaatgg caaccctgag aggcttcaga atggggcgct ggcttgccgg
    15181 caggctcatg aaagaactgg gactggtcag ttgccagcag cctgcgcacc gttataaacg
    15241 aggtggtcgt gaacatgtca ctatcccgaa tcaccttggg cggcagttcg cagtgacaga
    15301 gccaaatcag gtatggtgcg gcgacgtgac gtacatctgg acggggaaac gttgggcata
    15361 ccttgccgtt gttctcgacc tgtttgcaag gaaaccggta ggttgggcaa tgtcgttctc
    15421 tccggacagc agactgacca tcaaagcgct gaaaatggcc tgggaaatcc gcagtaaacc
    15481 agccggggta atgttccaca gcgatcaggg cagccactat acaagcaggc agttccggca
    15541 gttactgtgg cgttaccaga tcaaacagag tctgagtcga cgaggaaatt gctgggataa
    15601 cagcccgatg gagcgcttct tcaggagtct gaaaaacgag tggataccgg tgacgggtta
    15661 catgaacttc agcgatgctg cccatgaaat aacggactat atcgttgggt attacaacgc
    15721 gctcaggccg cacgaatata acggtgggtt gccaccaaat gaatcggaaa accgatactg
    15781 gaaaaactct aaagcggtgg ccagtttttg ttgaccacta catcccgtca gccgatttac
    15841 gggccgtaag taacgaagtt ggatgcaaat gtcagatcgt gtgcgcctgt tatggctcgg
    15901 ctggtaagag agccttatag gcgcatttga aaaaactcca gctataccgg aagagattta
    15961 ttattaacag gagaggtttg cttttctgtg taggtttaga ttttgtgttt gcagggtaaa
    16021 caaaactgat taactcgtca tttcggtaac tgaaatagtt tgctgtattc tgtttacaat
    16081 ctctttaata aggcatttgt cacgtggcta ttcatatgaa aatcatacgt tatcttacaa
    16141 atcaatcatc tgagtgttat aatataactt agctgtgata tgtgtaagaa tgtttaggca
    16201 atatattatt atcgctaatg ttgttttggt tgggagagat tctccttgcg gtagctttct
    16261 gtttttagat gcttcttgct taaaaggata taatttctgt tcttttatat agggttcttt
    16321 acatttactg agttttctta tttatttaaa cattatgaaa tctaatgctt ttgacgtcat
    16381 ggggaaggtt gcatggctat gggcctgttc tcctctgcat aaaaaatggc cattatctgt
    16441 ttttgctatt aatgtcatac ctgctattca gacaaatcaa tttgctctct taataaagga
    16501 tgagcttcct gtagcttttt gtagctgggc cagtttagat ctggaatgtg aggtaaaata
    16561 tataaatgat gtaacatctt tgtatgcaaa agactggatg tcgggagaaa gaaagtggtt
    16621 tattgactgg atagctccat ttggacacaa catggagtta tataaataca tgaggaaaaa
    16681 gtatccttat gagttattca gagcaataag gttagatgag agcagtaaaa caggaaaaat
    16741 agctgaattt catggagggg ggatagataa aaaactggca tcaaaaatat tcaggcaata
    16801 ccatcatgaa cttatgtctg aagtaaaaaa cagacaagat tttaatttta atattgagaa
    16861 agaaaactaa tatg
//
LOCUS       DAZKIF010000104        16703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZKIF010000104 DAZKIF010000000
VERSION     DAZKIF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.104"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..208)
                     /locus_tag="ACHOTW_003463"
     CDS             complement(<1..208)
                     /locus_tag="ACHOTW_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP9845506.1"
                     /translation="MILSTRISLGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLIL"
     gene            complement(355..1638)
                     /gene="yeaH"
                     /locus_tag="ACHOTW_003464"
     CDS             complement(355..1638)
                     /gene="yeaH"
                     /locus_tag="ACHOTW_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP9845507.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1751..3685)
                     /gene="yeaG"
                     /locus_tag="ACHOTW_003465"
     CDS             complement(1751..3685)
                     /gene="yeaG"
                     /locus_tag="ACHOTW_003465"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP9845508.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4121..4867
                     /gene="mipA"
                     /locus_tag="ACHOTW_003466"
     CDS             4121..4867
                     /gene="mipA"
                     /locus_tag="ACHOTW_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP9845509.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            4957..5811
                     /gene="yeaE"
                     /locus_tag="ACHOTW_003467"
     CDS             4957..5811
                     /gene="yeaE"
                     /locus_tag="ACHOTW_003467"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP9845510.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5862..6746)
                     /gene="yeaD"
                     /locus_tag="ACHOTW_003468"
     CDS             complement(5862..6746)
                     /gene="yeaD"
                     /locus_tag="ACHOTW_003468"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP9845511.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6830..7825)
                     /gene="gapA"
                     /locus_tag="ACHOTW_003469"
     CDS             complement(6830..7825)
                     /gene="gapA"
                     /locus_tag="ACHOTW_003469"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9845512.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8167..8580
                     /gene="msrB"
                     /locus_tag="ACHOTW_003470"
     CDS             8167..8580
                     /gene="msrB"
                     /locus_tag="ACHOTW_003470"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9845513.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8622..8894
                     /gene="yeaC"
                     /locus_tag="ACHOTW_003471"
     CDS             8622..8894
                     /gene="yeaC"
                     /locus_tag="ACHOTW_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP9845514.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9264..10340
                     /gene="ydjL"
                     /locus_tag="ACHOTW_003472"
     CDS             9264..10340
                     /gene="ydjL"
                     /locus_tag="ACHOTW_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9845515.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10367..11746
                     /gene="ydjK"
                     /locus_tag="ACHOTW_003473"
     CDS             10367..11746
                     /gene="ydjK"
                     /locus_tag="ACHOTW_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9845516.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11763..12806
                     /gene="ydjJ"
                     /locus_tag="ACHOTW_003474"
     CDS             11763..12806
                     /gene="ydjJ"
                     /locus_tag="ACHOTW_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFP9845517.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12827..13663
                     /gene="ydjI"
                     /locus_tag="ACHOTW_003475"
     CDS             12827..13663
                     /gene="ydjI"
                     /locus_tag="ACHOTW_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9845518.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13668..14600
                     /gene="ydjH"
                     /locus_tag="ACHOTW_003476"
     CDS             13668..14600
                     /gene="ydjH"
                     /locus_tag="ACHOTW_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9845519.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHCTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14610..15590
                     /gene="ydjG"
                     /locus_tag="ACHOTW_003477"
     CDS             14610..15590
                     /gene="ydjG"
                     /locus_tag="ACHOTW_003477"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFP9845520.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            15727..16485
                     /gene="ydjF"
                     /locus_tag="ACHOTW_003478"
     CDS             15727..16485
                     /gene="ydjF"
                     /locus_tag="ACHOTW_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9845521.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16603..>16703
                     /locus_tag="ACHOTW_003479"
     CDS             16603..>16703
                     /locus_tag="ACHOTW_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845522.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLT"
BASE COUNT         4365 a         3736 c         4201 g         4401 t
ORIGIN      
        1 ataaaattaa cgtcagcaat aaactgaaaa taaaaaagct ttgagctttt aatttttgtg
       61 tacccataga aaccataaaa aggagagcaa tgtgagtgct ggattttatt gatggcagag
      121 atatatagtt ttgaccagga tcaattttcg ttaacgataa aaaatattta aagaataacc
      181 ccagggaaat acgtgttgac agaataattt ttccgaatga ttgttttctg gaactaaatt
      241 gtgtaaaggt tatgctgtca tatttaaagc gattgtaagc taatgtatgt aataaatgag
      301 ataacttaca gtgagctaaa aatcagccag gcgataatga cccggctgat agttttagtc
      361 tttagctgtt gcattttgct tatgaaacag ttcacggaac accggataaa tatcatcctg
      421 gtcgcggata tgctgcatcg caaagttgtc gaaagtagat tgcagatgct catattctcg
      481 ccacagtgtc tgatgcgcgc gacgggtaat ttcgatatag ctgtaataac gaacaacggg
      541 taataatttt ttcgccagaa tttcatggca aagcggagag tcatcggccc agttatcgcc
      601 gtccgatgct tgtgcagcgt aaatattcca ctgtgccggg ttataacgct ctttcactac
      661 ctcatccatc agtttcaggg cgctggaaac aatggtgccg ccagtttcct gcgagtagaa
      721 aaactcatgt tcatcgactt ctttcgcctg ggtatgatgg cggatgtata cgacttccac
      781 gttcttatac gttctgctga ggaacagata cagcagaata taaaaacgct tagccatatc
      841 tttagtggat tgatccattg aaccggaaac gtccatcagg caaaacatca ctgcctggct
      901 ggagggatcg ggccgcttct cgtagttctt gtaacgtaaa tcgaaggtgt caataaaagg
      961 gacgcgttca attttggcac gtaattctgc aatttctttg cgcagacgtt cctcttccag
     1021 cagctgcgca ggttcactgt tgctgatgat ggccaaattc tcttccagtg catgaagttc
     1081 ccgccgcttg cctgccgtca tggctgtgcg tcgcgccagt gagttctgca aggaacgcac
     1141 aacgctgata ttggccggaa cgccgttagc ggtataaccc gcccgatgcg ttttatattc
     1201 ggtcagctgg cgttgttggt tttgtttcag attcggtaag gccaaatctt caaagagcag
     1261 atcaagatac tcatctttcg aaatctgaaa gacaaattca tcctgacctt caccatcctg
     1321 gctggcctgg ccctgaccac tgccggaacc tccgccgcca ccctggggac gttcaattcg
     1381 gtcgttctga acgaaatggt cattgcccgg atgcacgcgg tggcgcagac cgccacgccc
     1441 ctgatgaaac attggttcgc taatatcttc cgtgggaatg gatacggact cgccgctgtc
     1501 gacgtcagtc accgaacgct tattaatggc ctcggagatc gactgtttaa tttgcgcttt
     1561 ataacggcgt aaaaaacgct ggcgattcac catgcttttg tttttgccgt tcagacgccg
     1621 gtcaataaac caggtcatat gccccccgta ctacatttgc caactgtatg ctacgcctgt
     1681 gttgccattt ctctggccca cggttcgctg tcgttgtagg cccggtagca tgtgtaccgg
     1741 gccagggttg ttaagacgat ttacgtacgc gcaaatacca ttcgcacagt aaacgcacct
     1801 gtttacgggt gtagcctttt tccatcatac ggtcgacaaa gtcgtcgtgt ttcttctgct
     1861 cgtcggttga cgttttggcg ttaaacgaga taaccggcaa cagctcctcg gtattggaga
     1921 acattttctt ctcgataacc gtgcgcagtt tttcatagct ggtccagttc ggattgcgtc
     1981 cgctgttatt ggctctggcg cgcagtacga agttaacaat ctcgttgcgg aaatcttttg
     2041 gattactgat ccccgccggt ttctcgattt tctccagctc ggcgttaaga gactcgcggt
     2101 caaacagctg cccggtatcc ggatcgcgat actcctgatc ctgaatccag aaatccgcgt
     2161 aggtaacata acggtcgaaa atgttttgcc catattcgga ataggattca aggtaggccg
     2221 tctggatctc tttgccaata aactcggcat atttcgggat cagataacct ttcaggaact
     2281 ccagatagcg ttctgcctgc tcttgtggga actgctcgcg ctcgatctgc tgttccagga
     2341 cgtagaacag atggaccggg tttgccgcca cttctacatg atcgaagttg aacacgcggg
     2401 agaggatctt aaacgcaaaa cgcgtcgaca gaccgttcat accttcatcg acaccggcgt
     2461 agtcacgata ttcctgatac gacttggctt tgggatcggt gtctttcaga ctttcgccat
     2521 cataaacccg catctttgaa taaatgctgg agttttctgg ctctttcagg cgcgaaagaa
     2581 tggaaaaacg tgacagtgtt tcgagagtgc caggggcgca tggggcgtga gtcaattcac
     2641 tgtgattaag caatttctcg tagattttga tctcttcgga aatgcgcaag caatacggca
     2701 ccttcacgat gtaaacacga tcgaggaagg cttcgttgtt tttgttatta cggaaagtga
     2761 cccattcgga ctcgttcgag tgggcaagaa taatcccgtt gaacggcagg gcggagatac
     2821 cttccgtccc gttgtagtta ccttcctgag tggcggttaa caagggatgc agcactttta
     2881 tcggtgcttt aaacatctcg acaaattcca tgatcccttg gttggcgcgg cacagcgcac
     2941 cggaatagcc gtaggcgtcc gggtcattct gcgcgtaatg ttcgagttta cgaatatcga
     3001 ctttcccaac cagcgcggag atgtcctggt tgttctcatc accgggttcc gttttggcga
     3061 tagcaatttg ttgcagaatt gacggccaga ccttcactac ccggaactta gtgatatcgc
     3121 caccaaattc atgcaggcgt tttgccgccc acggcgacat gatggtgccg agataacggc
     3181 gaggaatgcc atactctttt tccagaatct gcgcatcttc ctgcggattg aaaagacaga
     3241 acggatgatc gttgaccggg ctacgctcac cgttggcgct caatacataa atcggtacga
     3301 gctgcattaa tgatttcagt cgctcagcaa gcgatgattt acccccaccc acaggcccca
     3361 gcagatacag gatttgtttc ttctcttcca gcccctgagc cgcgtgtttc agataagaga
     3421 caatctgttc aatcgcgtct tccatgccgt aaaactcttc aaacgccgga taacgtgcaa
     3481 tgacccggtt agaaaagagt cgagaaagtc tgggttcctg ggctgtatcg accatgacag
     3541 gctcaccgat agccatcaat agccgctcag ccgcgttggc ataagcactg cgatcttgcc
     3601 gacaagtggt aagaaactcc tgcagtgtga actcttcgtc cttggcagct tcatatcgct
     3661 ggcgatagtg atcgaatata ttcatggtat gccgtccttt cgttttttag cacaggttaa
     3721 gagccgttca tatgaatagt agaggctccc ggaagaggta aactgaacga cgtctcttgc
     3781 taaaacacag caactctcat gccattttat cgacgatgag aacaacgtgc ccggtgatac
     3841 accttcaaaa ttaagcgtag atggcaaatg gaaaacttgc ctacacggct ttcttaattt
     3901 caatggcata tcaacaactc atccataaat tttgcataat taatgtaaag accaggctcg
     3961 ccagtaacgc taaattcatt tggctgtaag cgcggtgtca tccgcgtcag gaaaattaaa
     4021 cagttacttt aaaaaatgaa aacgtaaaaa ggttgggttt cgatgtattg acgggtaaac
     4081 tttgtcgccc gctaaacatt tgtttaagga atgattaatt gtgaccaaac tcaaacttct
     4141 ggcacttgga gtgcttatcg caacgtctgc aggcgtagcg cacgctgaag gtaaattttc
     4201 cctgggcgca ggcgtaggtg tcgttgaaca cccatataaa gattacgata ccgatgttta
     4261 cccagtaccg gtaatcaact atgaaggcga taacttctgg ttccgtggct taggtggtgg
     4321 ttactacctg tggaatgacg caacggataa actttcaatt accgcttact ggtcgccgct
     4381 ttacttcaaa gctaaagaca gtggcgatca ccaaatgcgt cacctggatg accgtaagag
     4441 caccatgatg gctggtctgt cttatgctca ctttacccag tacggttacc tgcgtaccac
     4501 cctggctggc gataccctgg ataacagcaa cggcatcgtc tgggatatgg cctggttgta
     4561 tcgttacacc aacggtggcc tgaccgtgac tccgggtatt ggtgtgcagt ggaacagcga
     4621 aaaccagaac gaatactatt atggcgtatc gcgcaaagag tccgctcgca gcggtctgcg
     4681 tggctataac tcgaacgaca gctggagccc ttacctggag ctgagcgcca gctacaactt
     4741 cctcggcgac tggagtgttt acggtaccgc gcgctacacc cgtctgtctg atgaagttac
     4801 tgacagcccg attgtggata aatcctggac tggcctgatt tctaccggga tcacctacaa
     4861 attctgataa tgcatctttc cagggcattt tgtgcataaa aacagggcgt cacgccctgt
     4921 tttgcattac ggtggtgcaa tacctgggag gaaacaatgc aacaaaaaat gattcaattt
     4981 agtggcgatg tctcactgcc agccataggg cagggaacat ggtatatggg cgaagatgcc
     5041 agtcagcgca aaacagaagt tgctgcacta cgcgcgggca ttgaactcgg tttaaccctc
     5101 attgataccg ccgaaatgta tgccgatggc ggtgccgaaa aggtggttgg ggaagcatta
     5161 accggtctgc gagagaaggt ctttctcgtc tctaaagtct atccgtggaa tgctggcggg
     5221 caaaaagcga taaatgcatg cgaagccagt ttacgccgtc tcaatactga ttatctcgat
     5281 ctctacttat tacactggtc tggcagtttc gcttttgaag agactgttgc agtgatggaa
     5341 aaattgatcg cccagggaaa aatccgccgc tggggcgttt cgaaccttga ttatgctgat
     5401 atgcaggaac tctggcagct gccgggggga aatcagtgtg ccactaatca ggtgctttac
     5461 catctcggtt cacgagggat tgagtacgat ctactcccct ggtgccagca acagcagatg
     5521 ccggtgatgg cttacagtcc gttagcccag gccgggcggt tgcgcaatgg actgttaaaa
     5581 aacgcggtag tcaacgaaat tgcacatgct cacaatatca gcgcggcgca agtattgttg
     5641 gcgtgggtaa tcagccatca gggtgtgatg gcgattccaa aagcggccac gattgcccat
     5701 gtccaacaga atgcggctgt gcttgaggtc gaactttcgt cagcggaatt agccatgctg
     5761 gacaaagcgt atccggcacc aaaaggaaaa actgcgctgg atatggtgtg atgttgatac
     5821 gtgccggatg catcgcaccc ggcacacaac attgacgcaa attaacgttt ggcaacgcga
     5881 atggattgcg ccagatgtgc aggtttttct ttggtcactt tttgcatttc tgaggcgtaa
     5941 gctgtttcta cacaaacaaa ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact
     6001 gaaagcgccg gtcccgggtt ccagcctaca acgttcagat gatgctggtg gcctacggcg
     6061 ataatacgat tcagcgcttc atcattaatc acgctgcaat cttgcggatt cagatacacg
     6121 cgatcggtac ggtcagggaa ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca
     6181 tttactttat caatgaagcg atcgcccagc ccactgacgc ttaccttagc gatatcaccc
     6241 acgttaaagt aggtatgcag ggcagaggtg gtttcaaatt cgccgtgtgc ttcaagatcg
     6301 atttcacagt ttttatccac gcggaaatgc gctaacagag taaagtcgtg cagccagaat
     6361 tttttcgtct cttcgctttg tgtcaattca aaagtcagcg ctacgccatt agcatcttca
     6421 cgatgtgatt tcagcgtcca cggcaggttg cgggcaaaac cgtgcgcagg caggccttgt
     6481 tgtgccgccg gaccaaacca cggccagcaa accggtacgc caccgcgaat agcaacgcca
     6541 tttttgaacg gtgtgttgtt gctcaaccac agaacttctt cttcacctgc aggtttccac
     6601 gagagaaggt gtgcgccctg taatgcaaaa gaggctttta cctggggatg atcgactaca
     6661 atgaggtcca gttcatccag tttacgacgg gagaggacag gggagatttg ttcgatgacc
     6721 ggaagggcaa aaattttctt aatcatgacg cagtccttta acttcatttt atcaggtaaa
     6781 aaaaagagcg accgaagtcg ctctttttag atcacagtgt catctcaact tatttagaga
     6841 tgtgagcgat caggtccaga actttgttgg agtaaccggt ttcgttgtcg taccaggata
     6901 ccagtttcac gaagttgtcg ttcagagcga taccagcttt agcatcgaac acggaagtgc
     6961 aaacttcgcc gttgaaatcg gtagatacta cgtcatcttc ggtgtagccc agaacgcctt
     7021 tcatttcgcc ttcagcagca gctttaacgg cagctttgat ctgctcgtaa gttgcagctt
     7081 tttccagacg aacggtcagg tcaactacgg atacgttcgg ggtcggaacg cggaacgcca
     7141 taccagtcag tttgccattc agttctggca gtactttacc tacagcttta gcagcaccgg
     7201 tagaggacgg gatgatgttc tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg
     7261 ggccatcaac ggttttctga gtagcggtag tagcgtgaac ggtggtcatc agaccttcga
     7321 tgatgccgaa gttatcgttg ataactttag ccagcggagc caggcagttg gtggtgcagg
     7381 aagcgttgga aacgatgtcc tggccagcat atttgtcgaa gttagcgcct ttaacgaaca
     7441 tcggagtgtt gtctttagac ggaccagtca taaccacttt cttcgcacca gcggtgatgt
     7501 gtttacgagc agtttcgtca gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac
     7561 caacttcgtc ccatttcagg ttagccggat cacgttcagc ggtaacacgg atttttttac
     7621 cgttaacgat cagatgaccg tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg
     7681 agtcatattt cagcatgtat gccatgtaat cagcgtctaa caggtcgttg attgcaacga
     7741 tctcgatgtc agaacgtttc tgagcagcac ggaaaacaat gcgaccgata cggccaaaac
     7801 cgttgatacc tactttgata gtcatatatt ccaccagcta tttgttagtg aataaaaggt
     7861 tgcctgtaaa attacaaaaa ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga
     7921 caaatcaatc ctgtgcctaa gcattacgcg actgactcgc ctcactcttc ctttgggctt
     7981 gagaccacat ggggtcggcg ccccgaattt taaagggcaa ttacgataaa aatgtgattt
     8041 tcatcacgat ttaggtgcag ccatttcgtc gagatcaagt ttaggacaaa attttgccag
     8101 ttggtgttaa tgttttgtta gaatcagtca cgtaatgtga gcacgattaa agtgagatgt
     8161 gagcaaatgg ctaataaacc ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag
     8221 ttttacgtga cgcagaatca tgggacagag ccgccattta cgggtcgttt actgcataac
     8281 aagcgtgacg gcgtatatca ctgtttgatc tgcgatgccc cgctgtttca ttcccaaacc
     8341 aagtatgatt ccggctgtgg ctggcccagt ttctacgaac cggtgagtga agaatccatt
     8401 cgttatatca aagacttgtc acatggaatg cagcgcatag aaattcgttg cggtaactgt
     8461 gatgcccatc tggggcatgt cttccctgac gggccgcagc caacgggcga acgttattgt
     8521 gttaactctg cttctttacg cttcactgat ggtgaaaacg gcgaagaaat caacggttga
     8581 aaaaacgatt cagcaactta ttccacagga gcggaaaaat tatgaatctt gatgacatta
     8641 tcaacagcat gacgcctgag gtataccagc gtttgtcgac cgccgttgaa ctggggaaat
     8701 ggcctgatgg cgttgcgttg accgaggaac aaaaagagaa ctgcctgcaa ctggtgatgc
     8761 tgtggcaagc ccgcaataat accgaagcac agcatatgac aattgacaca aatggtcaga
     8821 tggtcatgaa gagcaaacag cagttaaaag aagattttgg tatctcagca aagcctattg
     8881 ccatgtttaa gtaagtggtt catccccttt ttgccgcggc tatgaagggg atagccaaca
     8941 atgaccccac gactcatctc aattatctcg ccatgacctg gctaagtccc agtccgatct
     9001 cttgccgtcg cgttcacgcc tgaatgactt ctcctgaact taagtctcag acctatttgg
     9061 ccggtaatcc ctctcaaatt tgctcctttt gcgttccgcc ttacatcaac ctgtaacaga
     9121 accagccatt ttctgcgtaa ttgtgatctg agttgtaaaa cgcagattat tatctgtgtt
     9181 ttacatgttt aatgttgacc tcaaaaatca tgtgggtaag tatgatgacc atctaagcaa
     9241 aacaaaacac agaggatgac gcaatgaaag cactggctcg gtttggcaaa gcctttggcg
     9301 gctacaagat gatcgatgtg ccacaaccca tttgtggccc ggaagatgta gtgattgaaa
     9361 ttaaagccgc ggcaatctgc ggcgcagaca tgaagcacta caatgtcgat agcggttctg
     9421 atgagtttaa ctctatccgc ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa
     9481 aagtcaaaga ctggaaagtg gggcaacgcg tcgtgtcgga taacagcggt cacgtctgcg
     9541 gcgtttgtcc ggcctgtgaa caaggtgatt ttctgtgttg tacagaaaaa gtaaaccttg
     9601 gtctggataa caatacctgg ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa
     9661 ttctcaaaat tcatcgtcat gcgttgtggg aaatccctga tggtgttgat tatgaggacg
     9721 cagccgtact tgaccctatc tgtaatgcct acaaatccat cgcgcagcaa tcgaaattcc
     9781 tccctggtca ggatgtggtc gtcatcggca ctggcccact cgggctgttc tccgtacaaa
     9841 tggcgcgaat tatgggggcg gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg
     9901 tccgcttccc ggttgcaaaa gaactgggtg cgacggcagt agtaaatggt tctaccgaag
     9961 atgtggtggc gcgctgccag caaatttgtg gcaaagacaa tctgggactg gtgattgaat
    10021 gctccggtgc caatatcgca ctgaaacaag ccatcgaaat gctccgtccg aacggggaag
    10081 tggtacgcgt tggaatgggc ttcaaacctc ttgatttctc gattaatgac attaccgcct
    10141 ggaacaaaag catcattggg catatggcct atgactccac ctcatggcgt aacgctatca
    10201 ggctattagc cagcggcgct atcaaagtca aaccgatgat cacgcatcgt atcggcctgt
    10261 cgcaatggcg cgaagggttt gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga
    10321 cttacgactt tgatgaataa ccactctcct gaaggaacgt tatcgcatgg atcagataac
    10381 aaaaccgcat tgtggtgcac ggctggatcg cttaccggat tgccgctggc attcatcaat
    10441 gtttgccatc gtcgcgtttg gcttgcttgt ctgctggagt aatgccgttg gtggcttgat
    10501 cctcgcgcag ctgaaagcgt tgggctggac agataattcc accacagcca cattctcagc
    10561 aatcacgacc gccggaatgt ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac
    10621 cggtcgcaga aatgcgttca tcctctatga ggccattcat attgcctcga tggttgtcgg
    10681 tgctttctca ccgaatatgg atttccttat tgcctgccgt tttgtgatgg gagttgggct
    10741 gggagcttta ctggttacgc tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg
    10801 tggaacgtgg tcaagtcggg tttcctttat tggcaactgg tcatatccgc tctgttcatt
    10861 gatagcgatg ggactcacgc cgctgattag tgcagagtgg aactggcggg tacaactgct
    10921 tatccctgca atattgtcgc ttatcgctac ggcgctggcc tggcgctact ttcctgaatc
    10981 cccgcgctgg ctggaatcgc gcggacggta tcaggaagcc gagaaagtca tgcggagtat
    11041 agaagaaggc gtcatacgcc atacaggtaa acctttgccg cccgtggtta ttgctgatga
    11101 cggtaaagca ccacaagcgg tgccatattc agccttactg acaggagtat tactgaaacg
    11161 cgtgatatta ggctcttgtg tgctgattgc catgaacgtt gtgcagtaca cactaattaa
    11221 ctggttgcca acgatattca tgacccaggg gattaattta aaagactcga ttgttttaaa
    11281 taccatgagt atgtttggca cgccatttgg catttttatt gccatgctgg tgatggataa
    11341 aattccgcgt aaaacaatgg gtgtggggct attaatcctg attgcggtgc tcggatatat
    11401 ctattcactg caaaccagca tgttgctcat aacgttgatt ggtttcttcc tgattacttt
    11461 cgtctatatg tacgtttgct atgcctcggc agtgtatgtc cctgagatct ggccgacaga
    11521 ggccaaactc cgtggctccg gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc
    11581 accttatgcc gttgcagtgc tgctcagtag ttatggcgtt acgggagtct ttattcttct
    11641 gggggcggtt tcaattattg tcgcaattgc tatcgccacc attggaatcg aaaccaaagg
    11701 tgtctccgtt gaaagtttaa gtattgatgc agtagccaat aaataatacg ggtggattca
    11761 aaatgaaaaa ttcaaaagca atattgcagg tgccgggcac aatgaaaatt atttcagcag
    11821 aaataccagt gcctaaagaa gatgaagttt tgattaaagt agaatatgtc ggtatttgtg
    11881 gttcagatgt acatggtttt gaatcaggtc cgtttattcc gcctaaagac ccaaatcaag
    11941 aaattggcct gggtcatgaa tgcgccggga cggttgtggc tgtgggaagc cgtgtgcgca
    12001 aatttaaacc gggggatcgg gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt
    12061 actgtctgga aggcaaatat aacatctgcc cggacgttga ttttatggcg acacaaccca
    12121 actaccgcgg cgcattaacg cactatctgt gtcatccgga gagctttact tacaaactgc
    12181 ccgacaatat ggacacgatg gaagggacgc tggtggagcc tgccgcagtc gggatgcatg
    12241 ccgcgatgct ggcagatgtt aaaccgggta agaagataat tattctggga gcaggttgta
    12301 ttggtttgat gacgttgcaa gcgtgcaaat gcctgggagc aacggaaatt gccgtcgttg
    12361 atgtgctgga aaaacgtctg gcaatggcgg aacagcttgg tgcgacagtg gttattaacg
    12421 gcgcaaaaga agacactatt gcacgctgtc agcaatttac cgaagacatg ggcgcagata
    12481 ttgttttcga aacagcgggt tctgcggtca ccgttaaaca ggcaccttat ctggtaatgc
    12541 gtggcggtaa aattatgatt gttggtactg tacccggcgc ttcggcaatc aatttcctca
    12601 aaatcaatcg cgaagtcact atccagacgg tattccgcta tgccaatcgt tatccggtca
    12661 cgattgaagc aatttcttca gggcgattcg atgtgaaatc gatggtgacg catatttacg
    12721 attatcggga tgtacaacag gcatttgaag agtcagttaa caacaaacgc gacattatta
    12781 aaggcgttat taagattagc gattaattaa tacgggagaa cagaacatgc tcgcagatat
    12841 cagatattgg gaaaacgatg ccacaaataa acgttatgct attgcccatt ttaatgtatg
    12901 gaatgcagaa atgttgatgg gcgttatcga cgcagccgaa gaagcgaaat caccggttat
    12961 tatttctttt ggcacaggtt ttgtcggcaa cacctcattt gaagatttct ctcacatgat
    13021 ggtatctatg gcacaaaaag caacggtgcc ggtaataact cattgggatc atggtcggag
    13081 tatggagatt attcataacg cctggactca tggtatgaat tcattaatgc gtgatgcttc
    13141 cgcatttgat ttcgaagaaa atattcgttt aaccaaagag gctgttgatt tcttccatcc
    13201 gctgggtatt ccggtagagg cggaattagg gcatgtcggt aatgaaaccg tttatgagga
    13261 agcgctggcg ggctatcact ataccgatcc tgaccaggcg gctgaatttg ttgaacgtac
    13321 gggctgtgac tcattggctg tcgccatcgg caaccagcat ggggtttata cgtcagagcc
    13381 acaattgaac tttgaggtcg tcaaacgcgt acgcgatgca gtgtctgttc cgctggtttt
    13441 gcatggcgca tcggggatca gtgatgccga cattaaaact gcaatttcgt tgggtatcgc
    13501 gaaaatcaac atccatacgg agctctgtca ggccgcaatg gtcgcagtga aagaaaacca
    13561 ggatcagcct ttcctgcatt tagaacgtga agtacgtaaa gctgtaaaag aacgggcatt
    13621 ggaaaaaatt aaactgttcg gttctgatgg caaagcggaa taatgatatg gataatctcg
    13681 acgttatttg tataggtgcc gctattgttg atattccatt gcaaccggtc agtaaaaata
    13741 tctttgatgt ggattcttat cctcttgaaa gaatcgcaat gaccaccggg ggtgatgcaa
    13801 taaatgaagc aacaattatt tctcgtctgg gccattgtac agcattaatg agccgtattg
    13861 gtaaagatgc cgcagggcag tttattctcg accattgccg caaagagaat attgatattc
    13921 aaagcctgaa gcaggacgtc aacatagata cctctattaa tgttggactg gtgacggagg
    13981 atggcgagcg gacgtttgtc acaaaccgta acggcagtct gtggaaattg aatattgacg
    14041 atgttgattt tgcgcggttt tcccaggcga aattattatc actggccagt attttcaaca
    14101 gtccactatt ggatggtaaa gcgctaacag aaatttttac gcaagctaaa gcccggcaga
    14161 tgaatatctg tgccgatatg attaaaccgc ggttgaatga aacgctggat gatatttgcg
    14221 aagcattgag ttatgtcaat tatgccgagg caaaattact caccgggaaa gagacactgg
    14281 atgaaattgc tgacagcttt cttgcgtgcg gcgtaaaaac ggtggtgatt aaaacgggta
    14341 aagacggctg ctttatcaag cgtggtgaca tgacgatgaa ggtgccggcg gtcgcaggaa
    14401 taaccgccat cgacaccatt ggcgcgggcg ataactttgc ttcaggtttt attgcggcac
    14461 tgttagaagg aaaaaatctg cgtgaatgcg cacgctttgc caatgcaacg gcggctatct
    14521 cggttctaag cgtcggtgcc accaccggcg taaaaaacag aaagctggtc gaacaattgc
    14581 tggaagaata cgaaggataa tgaaggcaaa tgaaaaagat acctttaggc acaacggata
    14641 ttacgctttc gcgaatgggg ttggggacat gggccattgg cggcggtcct gcatggaatg
    14701 gcgatctcga tcggcaaata tgtattgata cgattcttga agcccatcgt tgcggcatta
    14761 acctgattga tactgcacca ggatataact ttggcaatag tgaagttatc gtcggtcagg
    14821 cgttaaaaaa actgccccgt gaacaggttg tagtagaaac caaatgcggc attgtctggg
    14881 aacgaaaagg aagtttattc aacaaagttg gcgatcggca gttgtataaa aacctttccc
    14941 cggaatctat ccgcgaagag gtagaagcca gcttgcaacg tctgggtttt gattacatcg
    15001 atatctacat gacgcactgg cagtcggtgc cgccatttta tacgccgatc gctgaaactg
    15061 tcgcagtgct taatgagtta aaggctgaag ggaaaattcg cgctataggc gctgctaacg
    15121 tcgatgctga ccatatccgc gagtatctgc aatatggtga actggatatt attcaggcga
    15181 aatacagtat cctcgaccgg gcaatggaaa acgaactgct gccgctatgt cgtgataatg
    15241 gcattgtggt tcaggtttat tccccgctag agcagggatt gttgaccggc accatcactc
    15301 gtgattacgt tccgggcggc gctcgggcaa ataaagtctg gttccagcgt gaaaacatgc
    15361 tgaaagtgat tgatatgctt gaacagtggc agccactttg tgctcgttat cagtgcacaa
    15421 ttcccactct ggcactggcg tggatattaa aacagagtga tttaatctcc attcttagtg
    15481 gggctactgc accggaacag gtacgcgaaa atgtcgcggc actgaatatc aacttatcgg
    15541 atgcagacgc aacattgatg agggaaatgg cagaggtctt ggggcgttaa atactcaaca
    15601 cgattcacct ctgtttctgt gaaaactgtg atgaaaatct gtgattcaaa caggttattt
    15661 tgaaagtaaa catcggttat gcgataatcg cgctaatgtg atgtaaaaag atacaggggt
    15721 gtaatcgtgg cggcaaaaga caggattcag gcaattaagc aaatggttgc caacgataaa
    15781 aaggtgacag tctcaaattt gagtgggatt tttcaggtaa ccgaagaaac cattcgccgc
    15841 gatcttgaga agctggaaga tgaaggcttt ttgaccagaa cctatggtgg tgctgtttta
    15901 aatacagcga tgttgacgga gaatatccat ttttataagc gcgcttcatc gttttatgaa
    15961 gagaagcagc ttattgcacg caaggcacta ccctttatcg acaataaaac caccatggca
    16021 gccgattcca gtagtaccgt aatggaattg ctcaaattgt tacaggaccg tagtgacctg
    16081 acgttgctaa ccaactcggc ggaagcgatt catgttctgg ctcagtcaga aattaaagtc
    16141 gtttcaacgg gtggggaact aaacaaaaat acactttcac tgcaaggaag aattactaaa
    16201 gagatcatca gccgctatca tgtcgatatc atggtaatga gttgtaaagg tcttgatatt
    16261 aacagcggcg cgctggactc taatgaagcg gaagctgaaa tcaaaaagac aatgatccgt
    16321 caggcgacag aagttgcgtt attggttgat cactctaagt ttgatcgcaa agcttttgtc
    16381 cagttagccg actttagtca tattaattac ataataactg ataaatcacc gggtgcagag
    16441 tggatagcat tttgcaaaga caataatatc cagctggtgt ggtaatttaa gcacgcgttg
    16501 tcaatattat ttgttcctgc gcctggaaat aaaatgatga taaagatgcg gcaagtgaat
    16561 ttgccgcaat tgctaccttt tttagcaaat ccgaggcacc caatggaaca atatgatcaa
    16621 attggcgcaa gactggaccg cttgcctttg gcccggtttc attatcgtat atttggtatt
    16681 ataagcttta gtctgttatt aac
//
LOCUS       DAZKIF010000105        16040 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZKIF010000105 DAZKIF010000000
VERSION     DAZKIF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.106"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..281)
                     /locus_tag="ACHOTW_003480"
     CDS             complement(<1..281)
                     /locus_tag="ACHOTW_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9845523.1"
                     /translation="MQDIPQETHHETTRLTQSAQAVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSSARPTLTVSNLHGMVTGMAE"
     gene            complement(291..620)
                     /locus_tag="ACHOTW_003481"
     CDS             complement(291..620)
                     /locus_tag="ACHOTW_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9845524.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(620..3685)
                     /locus_tag="ACHOTW_003482"
     CDS             complement(620..3685)
                     /locus_tag="ACHOTW_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9845525.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGAVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEARKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            complement(3657..3986)
                     /locus_tag="ACHOTW_003483"
     CDS             complement(3657..3986)
                     /locus_tag="ACHOTW_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9845526.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(3995..4381)
                     /locus_tag="ACHOTW_003484"
     CDS             complement(3995..4381)
                     /locus_tag="ACHOTW_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9845527.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            complement(4442..5185)
                     /locus_tag="ACHOTW_003485"
     CDS             complement(4442..5185)
                     /locus_tag="ACHOTW_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9845528.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            complement(5196..5597)
                     /locus_tag="ACHOTW_003486"
     CDS             complement(5196..5597)
                     /locus_tag="ACHOTW_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9845529.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVSALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(5594..6172)
                     /locus_tag="ACHOTW_003487"
     CDS             complement(5594..6172)
                     /locus_tag="ACHOTW_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9845530.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAGSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLAQR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(6184..6459)
                     /locus_tag="ACHOTW_003488"
     CDS             complement(6184..6459)
                     /locus_tag="ACHOTW_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP9845531.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            complement(6452..6775)
                     /locus_tag="ACHOTW_003489"
     CDS             complement(6452..6775)
                     /locus_tag="ACHOTW_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFP9845532.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(6862..8889)
                     /locus_tag="ACHOTW_003490"
     CDS             complement(6862..8889)
                     /locus_tag="ACHOTW_003490"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP9845533.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLNINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGKPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(8834..9169)
                     /locus_tag="ACHOTW_003491"
     CDS             complement(8834..9169)
                     /locus_tag="ACHOTW_003491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9845534.1"
                     /translation="MASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIR
                     GGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEP
                     QHTDDQSEE"
     gene            complement(9291..10415)
                     /locus_tag="ACHOTW_003492"
     CDS             complement(9291..10415)
                     /locus_tag="ACHOTW_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9845535.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLNFFQYSAQL"
     gene            complement(10343..10555)
                     /locus_tag="ACHOTW_003493"
     CDS             complement(10343..10555)
                     /locus_tag="ACHOTW_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845536.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(10552..12654)
                     /locus_tag="ACHOTW_003494"
     CDS             complement(10552..12654)
                     /locus_tag="ACHOTW_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9845537.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            complement(12654..13145)
                     /locus_tag="ACHOTW_003495"
     CDS             complement(12654..13145)
                     /locus_tag="ACHOTW_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP9845538.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            complement(13820..13972)
                     /locus_tag="ACHOTW_003496"
     CDS             complement(13820..13972)
                     /locus_tag="ACHOTW_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845539.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(13960..14427)
                     /locus_tag="ACHOTW_003497"
     CDS             complement(13960..14427)
                     /locus_tag="ACHOTW_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9845540.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(14424..14921)
                     /gene="rrrD"
                     /locus_tag="ACHOTW_003498"
     CDS             complement(14424..14921)
                     /gene="rrrD"
                     /locus_tag="ACHOTW_003498"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP9845541.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(14921..15136)
                     /locus_tag="ACHOTW_003499"
     CDS             complement(14921..15136)
                     /locus_tag="ACHOTW_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9845542.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            15279..15677
                     /locus_tag="ACHOTW_003500"
     CDS             15279..15677
                     /locus_tag="ACHOTW_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845543.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(15758..15916)
                     /locus_tag="ACHOTW_003501"
     CDS             complement(15758..15916)
                     /locus_tag="ACHOTW_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9845544.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
BASE COUNT         3680 a         4752 c         3669 g         3939 t
ORIGIN      
        1 tcttccgcca tccccgtgac catgccgtgc aggttagaaa ccgtcagtgt cggacgggca
       61 gaactgccct tgccgttcag ttcaaatccc gtcccctgaa tggggtatgc ctgatactgc
      121 cgcccctgcc aggtaaccgg ctcacctttt tcgttctgct cattacagaa aaaataacgt
      181 tcaccaccga cctctgtcag atcgatttcc cagagcaccg cctgggctga ctgagtgagg
      241 cgtgtcgtct catgatgtgt ttcctgtgga atatcctgca tcagagcctc ctatgccacg
      301 acctgttcaa aatctgccgt tatggttacc cacagcgccc ccacgcttgc cgaccattta
      361 cgacaaacca ccctgatcgg tttccagtca taaggtggcg tccactgaaa tgcacggacg
      421 ccaccgtgcc gttccagaaa ggcttttaaa gatgggtgtt cacatttacg aacacgtatc
      481 gtcacgctgt aagtcgacaa ctggttattc agtcccgccg cacgacgctg ttcataacca
      541 tcgcccagct tcactgtcac cactttcggc tctgatacca cattcatatc cgggcgcact
      601 ttccagtgaa acgtctccat taccgatatg ctccacttaa ccgaccacca tcacgggcct
      661 gctgttgcat aaagtccgct gccgcttttt tcccgaggtc ataaaccgcc ttcagggcag
      721 ccggacctat ctgcccgttc gtgccatcgt tattgatctc gatgttgtac tgcggggaaa
      781 acatcgccat acctgaaccc ccaatatccg ccacaacccc cagcttaccg tcagcaccac
      841 gacgcaatgg cagaatggct tcaggtcccg cttcccccat cacacccgcg ccttttgcaa
      901 aagcaaaaaa cgtcggacgg ttaaccaccg tgccactgta acgactcaaa tcagcagact
      961 gataaatccc cccattggca ttcagggaaa ggctgctgag atcaaatccc agtacactgc
     1021 caatcccttt tatcgatttc atcatggttg cctgcgccag gattttcgcc atatctgaca
     1081 gcacagaaga ggtgaaggat ttgaaattga gtttgccggt agtgacaaaa gttgccaggc
     1141 catttcccat gttgctgaag gctgacgaga acacctgttc tgctgtttct gccgtattat
     1201 ctgcatccac ggtaaaatcc tgaaatgcac gcaggactcc gtttttccag ttaccctgta
     1261 caacttcaag ctgttgccag taacggcgat tctcattcag ttgtcggttc aggctctccg
     1321 tcagcgcctg ctcggccttt ctgtagtcat ccgtgttata tgtccctttc tgctcactat
     1381 cccgcctcaa ctgctccagc tgttgctggt atttctggcg aagactcagt tgtacctgat
     1441 atcgctgtcg ctgctgatca cccataccca ccgtggcgat atccaggtca tgttgctgac
     1501 gctgagcgcg ctcttcttca gccagttgac tggtcagctg aattgttttt ttcttcagct
     1561 cgttgagtgc cgtctgtttc tgaagctcct gctgttttac atccagcagc gtcagtgcct
     1621 gaatcagttc atctttacgg gccagcacac tcttttcatc tgccgtcagt tttttcccgt
     1681 ccaggtcgct gatgcgctgc tgcagagcca gaagctgttt acgcgcttct gtcatccttt
     1741 ccgtggcaat gcctgctgac tgtctggcag cagcaatctg tccttccacc tgtgcctgtt
     1801 gctgactgta ctgcagcaat aaccgggtgg cctcatcatt acgggtttcg cgtgtttttt
     1861 tcttaccgga tgccagggct ttctcgtaac gttcattttc acgttgtatc gccgcatccc
     1921 tgacagcctg atcggcgtac tgcatggcat taatacgcgc tatttcacgc tgatgtcgtg
     1981 ctgcttccgt ttcattcatc cggttcagtg cagcattttc agcattacgg cgtttctgtt
     2041 gctcctgata attccgctct gcctgctctt ttgcatcctg caaatccttc tggcgttttt
     2101 tctcctgaag atcgttaaga cgctgctgat cgtattcaac ctgagaagat gatgccgtcc
     2161 aggggagtct tttcgcccgc gacactttct cctgtaaagc ggcaatctgt tcatccagcg
     2221 agtcttcacg accaatattc atggccgcat cccagaaacg actccacaaa tcagacagat
     2281 acttcagcgt actgccaagc gcattgaggt tattatcaat atccgcagta cgccgaccgg
     2341 tttcctctgc cagtgcagac atggctatcc gtgcagcatc actggaccgc ccctgctccc
     2401 caaggacgcg tatctgctca agctgagtgg cagtaagaaa atgcagctca ttgtccagag
     2461 ccttcgcggc atttacagga tcatccttca gccgcttaaa ctgatttatg gtatcgctga
     2521 ccgactggcc aaccgatcgc tccatctgtg cggcagctct cgccaccata ccgatatcgt
     2581 ttccacgaaa tgcaccactc cccaccacct gagccagcgc accggctgca gcatgttgcg
     2641 tgataccatt cccggaaata gcacgactga gcgtccacag ctgcccggca gtgactccgg
     2701 cataatgccc cgtcagcgac agctggcggt taaattcttc cccctccttc tgaccgtcat
     2761 accaggcttt acccagacca tagacagccg cggcaatacc gccaataacc gctcctggca
     2821 tcatgccttt cggtgacatc agtgtgtcta tccacccggc acggttagcc agcgttattc
     2881 cggatcccct cagcgcacct aaattgccgc gggccagttc acctatcaga acgcctatct
     2941 cctgacgggc tgctgcactt ttcagaccca gcgaatgcgt ggcttttcct gcctgctcca
     3001 ttttgcggat atacacttct gcagcactgc ttacccccag ctgggcagcc ttagcacgaa
     3061 gcaactcaga agaagaaaga ttctggcggg ttgcctgctc tttaagctga cggataaacg
     3121 ccactttctg tcgggtagcc tcttcctcag cctgtgtaag aacacgggtt ttcgccgtaa
     3181 cctcagaaat cagcgccaga taatcctgct gaccaatccc gccactgttt ctggcctgtc
     3241 ggatctgttg ctgaatacgc tgtaattcct gcagccccgc accggcctgt ttcacactgt
     3301 caatctgacg ataaaacgca gcagccgctt tatcctgagc ctccgccaga gccatggcct
     3361 gcgcctgttc ctcgcgcatt ttctggctca gtgcctccat acgctggcgg gtttgctcca
     3421 cctcgcgggc catgcgttca tgagcctgtg cgctcttctc caccgtctgc gcatggacgg
     3481 atgcggctgt tgcagccgaa gaagccgcct gcattgtctg ccgggcggcc tgagtctgac
     3541 gctccataaa acgctgcata cgggcagaag accgttctgc atcgctggct gcaccattca
     3601 gaaggttttt gatacgggga atttcatttt taaactctgc cgcatcaatc cccaaatcaa
     3661 tgaccaggtt ggctatctgg tccataacgc acacctccgg aaataccttc cccaagatgc
     3721 atcagttctt cgtccgttcg ctccggtatc ccgttctctt ccggtaaaag gctgaaatca
     3781 gccaccgcag catcactgct accggacacc attctcacga tcaatgcctt cagcgaggca
     3841 aactgcgcat ccatccacac atcactgaag ctctgcatcc ggaaataatc gccccactca
     3901 ccaagctcag tggccgacat ttccgacagc atccgccgcc agtctgcccg ccggaactcc
     3961 cgggcaagcc gcatgacaaa ctgcatttcc cgcgtcagga cttttccggc gtcagcacct
     4021 catgctccaa atccccggca ttctcaatgg ctcccatacc gctcagcgac agaaccatct
     4081 ccgcccccgc tcccagggca tcatacgacc atgttgtaat aacggatgcg caaagcgtct
     4141 caacatcctg agactgttcc gcattccaca gtgagcggga aaccagccaa gcattgatat
     4201 ccatccccat ccgcagaaaa gcaatctgtc gttcagcctc cggcagttct ccctcttcgg
     4261 catcaaactt tgccgttcgc tgctgaacaa acgccagata ttcaattctc tgcagcccgg
     4321 acagctcact gagcaccacg gactgctttt cataattaaa cgtgccctgt ttcagaaaca
     4381 tcatgttctc cacctgcaaa aaagccccgg ataaccgggg caaatgatga gtatcgtcct
     4441 gttaacctgc agcgctgaca gtcaccgcag ccacagccac aaaattcccg tcagcggtca
     4501 tgcccacaat gctgacgctg ccctgcttca cgcctttcac cgtggccaca agtccgttca
     4561 gggtcacagt ggctgtctgt ggatctgtcg aatgcacact gatcgctttg tcactggctc
     4621 cgtcaggttt tactgtaaag gtcagcgtgg tggttgctcc cacattgaca ctggcggatg
     4681 ccggtgccac cgtcagcccg gtaacgccca cagtttcagt gccctcctct gccagatatg
     4741 gacgccccac accgctgatt ttcactgtgc gggtcatcac gtcttttgag gcaatggttt
     4801 tacccagtga gctcagccag ccgcggaaaa catcaacagt gccgttggga tatttgatac
     4861 gaaacgcgca gacttcaccg gagtcgaaca actgaaccag ttttttctgc ccgctgtcac
     4921 ccggacgcca ggccagcgtc gccgaagtat caccaacgga tttctgcccc tgggttgtcg
     4981 ttttccagtc tgcatcttca tcatcgagat aagtgtcatc ttctgcatca gcggtcattt
     5041 cgccaggttg cagatccttc accatcgcaa gacgcagcca gtcagtgtcc gacaaagggt
     5101 tcgcaaacgc atcgccgttg ccggtataca tccagaacgt cgtccccgca cctttcgtct
     5161 ttgccagtgg atttggcgtg gtcattacca cctccttaat tcgtgtacgt gatctggtac
     5221 gtgatttccg ccatcgccca ggtggccatc tcattatcac gttgatagtt aaaaccgagt
     5281 gggatcaggg tgtcgatgag tccggaaagt gccgatacat cattcagggc cgggaaaatg
     5341 gtgctctcca tccacatatc cagctctgaa tccggtgcct gtgcccggat gaagacagca
     5401 atatgcagaa cagcctgcca gtcatcttca tccgtcattt ttccggtgta ctgagcatca
     5461 ctcagccaca ccgccacggc aggcagttcc tgcgcatcaa caaatgccgg aagcccgtca
     5521 aaaaacgtgg cgctgtctcc acactgttcc cgaaggcgtg ccagtacgac ctggcggatt
     5581 tgtgtatgtc ggttcatcgg gtcagccata acctcagttg ttgtttcagt gcatacccca
     5641 gctgtttcgg catttccgca gcaatgatgc ggtcgcgggc atcttcaaat gcctgtgtca
     5701 gcggtccgga cagcgggatt ttcaccacat caatggggta acgatttttg ccatcaatac
     5761 gccgcatcac atgccagcga ccattcgcca gttgctgaat aaacgcatcc cggaaaagat
     5821 atttacccac cttcagcaca ctgccacggt actgcagttt tccaccacgc tgggccagtc
     5881 tgacccgggc tgtccccagc ttaatggcgg gcagattgcc ccggttaacg cggatcctgg
     5941 ccgtcatttt tcctgacgga ctggctttaa acacccggac acgctgacga accagtttca
     6001 gggggatccc tttcacctgg ttatctcccg caacggtatt cccggcaacc tgccgggtgg
     6061 caaccgagcc cgctttctgt gccacacggt ttatcgccca tgcgctggcc tgtggcacca
     6121 tacgggtatc aaggctgttc agattgcgga tggcattctc aagccccttc atcccacacc
     6181 tctttactca ataaagatca ttggcttacc gttaaagcgt tcatgccgtg tgaccgtcca
     6241 ttgttgtccg tcataaacaa cgcgatcccc gcgccgtggg cggtatcccg aagaaaacac
     6301 caccagagag accgcaggtc cggacagagc attcagctct gccagtgttt ctcccgggat
     6361 cacagtcata tcgacatcat taatcgaggc tgtctttccc atctttctga ccgtgatagc
     6421 atccatacgc gctgccagcc gggaaaaggg atcagacatt gagttttacc ggcacttctt
     6481 ctgcactggt tccggcatct gcccagacaa ccccgaccag cggatcagag ccgctgttag
     6541 tcagctgaac ttttcctgac ttcagataaa ccttcttacc cgttttcatg tcatccgttt
     6601 tcagcttagg cagcataaac acaccttcgg tcatgccgtc gcctgtttca ccctgtggaa
     6661 tatcggtcag cgccaccgca aaaacatcgc ccacctgcac cagatctccg ctgctgatgg
     6721 ctgcactggc aacaatcgcc accgtttttc cttcttctac aaaattcttt gccataactg
     6781 tctccgcaca gccccgttca ggggctgatt tcaggtacaa aaaaagccct tacgggccat
     6841 cagagttgtt gtctgcgacg tttacgccgt acatttcacc agaccgcggt gatcaactgg
     6901 cgcgacaccg gcgtcaatac gcactttcgt tgtcacgcca tccacactga agccctccat
     6961 ctgatcaata tatggcgtat ccacaccgtt gagataagcc acttcaatcg tatcggagcc
     7021 ttttgacgca gccaggtaga aggtggtctg gctgttatca tcaagacgag gctctgcaat
     7081 aacggtcgca aaatctttca ccgggttaat aataccggcg ttaatgtcag cccccttgac
     7141 acttgaggag cgaatgacct ggttagcaac agactccatc gccgtcggta ccagtacgaa
     7201 cgcaggacga atattcagat gacgctcccc ctctttctga acgcgcatca actggcgggc
     7261 tttatccagc gatgccacgt ccattgcagc gctctccagt acgtttgcat gtttcgcttt
     7321 atcgaacaga cttacattat ctgtggagat tttcgggtta gacgtcagaa tggcataaac
     7381 cagatcggca atagtggatt tcgccgcacg gcccagtttc atcgggacat cggtcagcat
     7441 attcagatca tcattgataa tggcctgacg ggtgatactg aacagctcgc cataggtcgc
     7501 cagtgcaata gtggcctgtt tatctccggt ggtgacgtat ttatattccg ccccttcacg
     7561 cacctgacgc agagcactga agccccccat acccacacga tgggcaattt taaaatcaga
     7621 caactgacct ttccgcgtcc actgttcata ggtttcaggg gcatcttccc agccctgcag
     7681 aatggctttg ttcgcaacat ccagcagaat attaccgaag tcagacgtac tgtgtgtgaa
     7741 cgccgcaccg accatctgca tcgggttata actggaaacc ccaatacccc gttcagtcag
     7801 tgacatacgg gcatattcac gcagggtcat cccgttgtag acattatcac gttcggtttt
     7861 ttcaaatccg gcacgcgcca tcagcgcctg gcggatccca tcccccacaa aattaccgtt
     7921 tccggcataa atatgagccg gggtattttt attagatggc gtggactcgc gccccatctc
     7981 gttcaacagc ttttcgcggg cctgctccag cgaacattca ggatcggcaa gacactgagc
     8041 ctgcagcgtc tgataacgcc cgccaaacat ggcaaacaga tcattaatac cgtttacacg
     8101 cgctttttgc tctgccagta cctgcgcacg gatactgttt tcatccacca caggtgctgc
     8161 tgcctgcact ggcgtccggg aggctgcagg tccatcatcc tgtacgcgtg gagcactgtt
     8221 gcgtggcgga gtaatcatgt ttcgaatgga ttccggcatc tttttaaatt cctctgtacg
     8281 ttttgactga atacatgcca ttgccttaac ggctggcgtc acctgatcag caaatccatg
     8341 tgccagacat tcggcaccgg acatccaggt ctcatccgcc agcatggcag caatttcatc
     8401 ggtggttttc ccggttttct gtgcataagc gggtaacaga accgcctcaa ctttatcgag
     8461 caggtcggca taggtgcgca tgtcctccgc atcaccgccc gtaaagccaa atggtttatg
     8521 aatcatcatg aaggagtttt ccggcataat gaccggtttt cccaccatcg caatgaccga
     8581 cgccattgac gccgccacac cgtcgacata aacggtaatg gacgcaccat gtgttttcag
     8641 tgcattaaaa atggcgatgc cttcaaagac atcgccaccc ggtgaattga tattgagatt
     8701 aatgtgggtg atatcgccca gtgcattcag atcactgata aactgcttcg ctgtaacacc
     8761 ccagaaacca atctcgtcat aaatataaat atccgcgtca ctctggtgac cagcctgcat
     8821 cctgaaccag gaattattct tcggactggt cgtcggtgtg ctgcggctcc tgtcgtttcg
     8881 ttgcggcact gctgcctcct ttatcactgg ccggatcggt atcaaatacc agatccagct
     8941 tgcggttttc atcaatttcg gccttgcgcc gacgtttgac atcatccgga ttacgaccac
     9001 ctgcacgtac ccagtctgat tctgtcgctg ctccaccacg aatctgaatt ttccaggcct
     9061 cagcctcctt aacagggtca atccacggca tcaccggtcc ggaatacacc gcggtataca
     9121 gtgaagaacg gtcaagatcg cggggtagcc tgataacacc ggatgccaca gcctgtttca
     9181 gccaggcacg atacatcggg cgggtgacgg caccaataaa ccagtcctgc aggatcaggt
     9241 agccatcagt ggattcaacc agctcctgac gctgggcgct gtaagtgccg ttatagttgc
     9301 gcgctgtact ggaaaaaatt cagacgactg cccgccgcca cggcacgcaa ctgaccatta
     9361 cgaaaagttt caaggttagg attggggcga tccgacttca ccattccgat ttcttcgccg
     9421 ggtttcagat cgtcgtaaat aatgcctggc tgaatggtaa gctcgcgttc cttatccttg
     9481 ctgccattac catccggttc atagctctgc ccatcgcctt tccggatgta catccccaga
     9541 gcagcggcga tccttgctgc agtcagctca gaatcctcat actctttcag ggcactgagg
     9601 cggatcagca caccggacaa caaagacgtc ccgcgcatct ggtgcagacg gcgaacaaat
     9661 ttaagatgca gcattcgctc tgcatccact tctttggttt ccatctgccg tccggatacg
     9721 ggacggcttt tatacaccag atatttttcg ggacgccccc agtcatcaac aaacacgccc
     9781 tgattcagcc tgttgctctc atcactggtc atgggaataa agtctggctc gagcgcctcc
     9841 agccagaaat gaacaccggc agaaggcgtc aggctgttta tgcgcccgga aaccatctgg
     9901 gcaaacacct caccatcgcg cagccaggta cgcagcatca gacgttccag catcggacgg
     9961 gtaaactgcc cggtgacttc cggactgaca gaccattcac tccatcgggt gcgtatctcc
    10021 gctgccagat cacgggcaat ggccccattg cgtaataccg gatgtggctc gacaataatc
    10081 ccgtttttcc ccaccacccg ttcttccagc ttgtcaaata caccaataac cagatcgtgg
    10141 ttgttatcaa ggtaacgggc ctgctcacgt aacgacacgg ccccgtactg gcttaactgg
    10201 tcggcagttc ggttttcccg ccgggctttg tgtgtccgcg tcgtttttac ggcctcataa
    10261 gcctggatca ccgcacggga acgcagcctt gccgctttcc atcctggtga aaaaacgcca
    10321 atcacatcat caagaattgc catcagaacc tcgccagccg gtacccggga tgcccccgtc
    10381 gtcgtgtaat cagagccgca aggcggcgct cccactcctg ccgtccctgc cggatctcag
    10441 ataagttttc catggtcatc tgctgaccat taaaggtgac ggattttccg tccagcaccg
    10501 ccatttcagc ttccgtataa cgctgaatca tggcttcgat atcattctgg ttcataacca
    10561 tcctccggaa gtcagccagg ggttaacatc gtcagttact gttttcttcc gtttttgttt
    10621 tttaacaggc gtggataccg gttccggtga gggtgacggt tcggtactgt ccgggacact
    10681 ctccagccag gtttcccggc tcgcccaccc cggtgcatcc ggccagcgga tcttttcgta
    10741 tccatgcaga atgaccagag cctcggcata caccatcagg tcaaaagctt cgttggcacc
    10801 gcgacccggc ttactccatt tcccgtcact actccgctct tcatacgtca gttcgtcgta
    10861 aaaccagctc cccagccagt cagggaaatg cacatagccg ggacctggag agtcacgcca
    10921 taacgcgtta ttcacccggt ctttcagtgc atccgtctga agaagccaga gcggcacatc
    10981 acctgcggcc tgcgcccgtc ggcccgttcg tccggtgtta tcagggaatg tacgggtgat
    11041 cagttttgcg cgccggatgc tgtcgccctt aaacaggtaa atacgtttac caaggccatc
    11101 acgacggcaa cgacgccaga atttataggc attatcagtg accccgtctt caccgccgga
    11161 gtccaccgcc attgccatca gtcgcatttg ttgagaagga tcggaggcca gcggccagct
    11221 tttatgaaaa acatccgtca gcaggacatc ccagtcttcc ggatagctgg ccggatcaat
    11281 tcgctggctc tcaccgtcgc tgtcaccgcg caatgactgc gtgatgttgt aacgatcaat
    11341 aatccagcgt tcgccacggc tgccatagcc cgttacctga accacaaaac ggcgatgacg
    11401 tcccgcctgc acatccactg tcgccacaag gaaattaacg ccatccggca cactgctgga
    11461 aggaactggc tctgcccgct gctcaagcag ttcacttttt cgttgctcca tgctggcgcg
    11521 gggaagataa ggtaatcccc agtcggtatt gataaccgtc ttgagtgttt cttcacttcc
    11581 ggttgtctcg tattcctgtt ctgcagtaag cagtttgtaa acgagttgcg agagtgtctg
    11641 gtaagcagct gccggaccct ccatccagaa tgacgcaata cgtgagcgtc ggggatcacc
    11701 ataacgacta ccatccgcat tgatggattc accatcccgc aaccagaccc cacgtccgtt
    11761 cagctcacgt ttttgttcag gcataatccg tcctgaacag gaaggacact gaatataagc
    11821 cgcctcactt gccagcacgg gatcggcaat atcacggaaa ccagcaacca catcgccgca
    11881 gggctgaaaa tactcaccac agtgtggaca gggccagtac cagcgacggc gatcgccacg
    11941 gttatagagc gacagtatcc ccgtggttgg tggagcctca tgcggtgaag tccgtcgcca
    12001 tttcacatcc ttcacatccc tgccggggga actctccacc agcgtcatac cactggacat
    12061 aaatgtggtg gtacgttttg aggcaagaga gaaggcatcc ccctcgccat caatatcttc
    12121 cggaaaacgg tcataatccg tcagcgccac gcatttataa tctgatgagg acatgatatt
    12181 gactgacggc cagccgattt tcaggtagtt gccagcaagg aatgttctgt cataaacgtt
    12241 gttgtcattt ttgttcggac tcaggcgact gaccacttcc gggctgacgc gaaacgttct
    12301 ggcgagtcgt tttttggagt gttcgcgggc tttttcctcc gtcatctgaa tgatcagcat
    12361 atccgcagga tcgcaaatca cgttgtaaat cacccagccg tcaatcaggc cgatagtctt
    12421 gccggttcgt gccgggccaa caaatatcac tgcgtcgtat tcacgcgagg ccaggcagtt
    12481 catcggctca ataacatacg gtgccaccag cggatcccac gggactgagt tcccggcccc
    12541 catgggcacc cgcatatact gagcaacggc atcagcaacc cgcattcgtc tcggtgcgcg
    12601 aaggatataa cctgaatcgg ttcgtgctgc ctttgcggtt tcctgattca gcattactcc
    12661 tcctgctgta attcctcctc atcatccgca cctgcttcgg tcacccgcag ggctatctga
    12721 tcgcgcagat catcaataat ggactgaaca cggctcacag cggcaggctg cagaccacag
    12781 tcacgttcca gaatatccgg taatgtctcc agcacctgca cgaccgcttt tgcccagatg
    12841 gcaaactccc gtctgacatc actggccgga atgagttgtg ccgtttcctg ttcgaactta
    12901 agacgctcac gttcagactg ataccaggct ttgcgttcat gtggatccat ttcgccctca
    12961 gcaaccggcg gtggtaaccc cataaattca gtcagaatat cggtcaaccg gtatagcttg
    13021 agtttgtcat gtccaccagc gggacgaatg tttttcagtc ttgccacgac agtctggcgg
    13081 tgcagaccag ataaagccgc cagttgatta atattcagca ccaggttttt caactcatga
    13141 tccatatttc ctccggagag ctttaaacat gcatcgtgcg aacaacttta agaaaacgcg
    13201 ttcgatgtcg aacaaaaaac actcaattcg acatacaaaa aacaaataac cattaataat
    13261 caataagatg caaagatgat ggtggccgat aaaaatgcaa aaactagcct ttttccgcga
    13321 cgctcccgcc ccgtggcagg ccaccccacc gggaggagcc gtcagcctga aagccatgac
    13381 gaacgtctga tacagccctt gcatgaatgg catcgggata atccagaaag gaatagcatc
    13441 gacccacaag aatctgtgtg agtgtcctgt ttcttccacc cccgcacagg actggcgagc
    13501 atgagggaca aacccgcgaa tcattagcgc ggtaaaaacc cggtgtgcat cgtttttgat
    13561 tattcccgca cactcgcgca gaaggagttc cccgtcgggc tacggtcatg gttaatgcgg
    13621 gaatacagcg acgatacagc gcatgatgtg tcaggcttga atacctttat ccgttaaaag
    13681 ggatatcagt taagttatcc cgtgtagggt ataagccatt atcaaagcca ctctgtaggg
    13741 agtggctttt gtaatggcaa taaaaaagcc ccgcgaatgc gaggctaaat ccaggtattt
    13801 ttaatgactg gctcttatct caacgcagcc ccttaccgcg cgccagatgc tcaatatcaa
    13861 gcatcagcaa tgagatgttt aaccatgatt cactccagaa gtgagcacca ccctgtctac
    13921 agagccagat gtgaaggatg atgagtaaaa ttatcgctat catcgaaggc attgcgtcct
    13981 gatgtattcc tgaagcgttc tcagtgctgt ttggtcgcgg ataattccgt cccggatacc
    14041 gagaacgttt cgtccagcaa ctggagagag ttcgacggtg gcatcattgc ccatgccgga
    14101 ggcgctggag gtttcgtctg aggatggcac agggcatttt cctttgacga acacccgacc
    14161 accattatca agcttgcgcc gaagagcatc atttttagct ttcgcatcag ctaactcctt
    14221 cgtgtattta gcatcgagtg catcagcagc acgctggcgc tgctgcatgt cagtaatggt
    14281 ggcggtcgcc tgcttcagct cactgacttt tttatcacgc tgttctttat aggcgatggc
    14341 gttatcacgg taatgattaa ccgcccatga ctggcagacg atgatgcaga taaccagagc
    14401 ggagataatc gcggttactc tgctcattgt tgcccccaca aacagacttc acgctcaatc
    14461 tcgcggcgag tcatcagccc tttccattgc ttaccgccag cgtatgtcca gcgccgtagc
    14521 tgatcacatg cgcctttgat atcaccctgg tttattttgc gaagaagcgt cgatgttctg
    14581 aaattaccag cgcccacgtt gtaaacgaac gagtaaagag cgccgcgcgt tgtttccggt
    14641 atatcgactt tgatgtacgg gttaatttgt ctggcgaccg tggcaaggtc tttattcagg
    14701 agggctttgc attctgcttc ggtatacgtt ttaccgggaa tgatgtcttt tccggtgtgt
    14761 ccgtgacata cagtccatac gccaacgata tcttcgtatg gtatgtagct gacaccttcc
    14821 aggccatcgt caccactcgg accagtgatg agcacagacg ctatggcagc agccccacca
    14881 ccaatagcag cagcaacagc cttgcgtaat gatggcgaca ttattcacct ctcgcagcct
    14941 tacgcttatc ttctttaatc ttgaaataaa ggtttgtcag atacgtcagc aagccaaaca
    15001 ccaagctacc caatacgcct attgccaccc actgggatgg ggagactttg tccagcaact
    15061 gcagtagcca gtatcccgtc cccaccgctg acgtggtgta tgacacacct gttgttattt
    15121 tttccatctg gtacataccc cgtctcccgt tatccggaag ctgacaacaa taaaaagcca
    15181 ccagttaatt cctgatggcc ctgatgcata aacgtcataa tacctgactg ttatgattga
    15241 caataatgat aatgtttata tagaaaggtt cccgatgtgt gttacatatc atttctccac
    15301 ggggaatatc cccacgccag cgcagactct tttacccgtt ctcttctgcg ctggctcttt
    15361 tttattatgc tgctgcattt acctctggca ccatgctttc tatctcaaca caatacgtgg
    15421 tacttcttgt aaccaatatc ataacgatta atcgacatag aatttctccc gtgtacagga
    15481 acagagttaa aaagccggaa ccggaatcaa atcacaggat gaccatctgc cagtggcagg
    15541 tcataaaaaa aaggccgcgc catgcgcagc cagaactcac aaggaaaatg atagaaggaa
    15601 ataacattag tgatgtacgc atggcgcctc ccgctaagtt ctgcaatgat caaacagaac
    15661 tcgctacgtg cccttaaaac tcgatcattt agcccctcca aggaggattc accatgcggt
    15721 tgatttttta ataaacagta aacaaaaaag tcaagaatta ttcattctgt tctttcatca
    15781 tcggccacag caataccaca atgccgcaga ccagagcgcc atcagtcagt accaacatta
    15841 tcctgctggt gaaatccatc atcaccatca ctaaaagcag gatcacaaca gcaagcagac
    15901 acagtttata aaacaatgtt caggaaacgc attcagcatg cctaaggttc tattcctacg
    15961 aatagccaac ttgcaactta aaatattatt tatgcagcca attaaattct ggtccttaca
    16021 atatcaacct gaagattctt
//
LOCUS       DAZKIF010000106        16022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZKIF010000106 DAZKIF010000000
VERSION     DAZKIF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.107"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(96..1145)
                     /locus_tag="ACHOTW_003502"
     CDS             complement(96..1145)
                     /locus_tag="ACHOTW_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9845545.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            1277..2494
                     /locus_tag="ACHOTW_003503"
     CDS             1277..2494
                     /locus_tag="ACHOTW_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9845546.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNDIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(2498..3055)
                     /gene="dcrB"
                     /locus_tag="ACHOTW_003504"
     CDS             complement(2498..3055)
                     /gene="dcrB"
                     /locus_tag="ACHOTW_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP9845547.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(3128..3793)
                     /locus_tag="ACHOTW_003505"
     CDS             complement(3128..3793)
                     /locus_tag="ACHOTW_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP9845548.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            4014..4259
                     /gene="tusA"
                     /locus_tag="ACHOTW_003506"
     CDS             4014..4259
                     /gene="tusA"
                     /locus_tag="ACHOTW_003506"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP9845549.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(4361..6559)
                     /gene="zntA"
                     /locus_tag="ACHOTW_003507"
     CDS             complement(4361..6559)
                     /gene="zntA"
                     /locus_tag="ACHOTW_003507"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP9845550.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(6633..7259)
                     /locus_tag="ACHOTW_003508"
     CDS             complement(6633..7259)
                     /locus_tag="ACHOTW_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP9845551.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            7400..7759
                     /locus_tag="ACHOTW_003509"
     CDS             7400..7759
                     /locus_tag="ACHOTW_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP9845552.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(7762..8031)
                     /locus_tag="ACHOTW_003510"
     CDS             complement(7762..8031)
                     /locus_tag="ACHOTW_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP9845553.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(8021..8617)
                     /gene="rsmD"
                     /locus_tag="ACHOTW_003511"
     CDS             complement(8021..8617)
                     /gene="rsmD"
                     /locus_tag="ACHOTW_003511"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP9845554.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            8767..10263
                     /gene="ftsY"
                     /locus_tag="ACHOTW_003512"
     CDS             8767..10263
                     /gene="ftsY"
                     /locus_tag="ACHOTW_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9845555.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            10266..10934
                     /gene="ftsE"
                     /locus_tag="ACHOTW_003513"
     CDS             10266..10934
                     /gene="ftsE"
                     /locus_tag="ACHOTW_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9845556.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            10927..11985
                     /gene="ftsX"
                     /locus_tag="ACHOTW_003514"
     CDS             10927..11985
                     /gene="ftsX"
                     /locus_tag="ACHOTW_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9845557.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGNGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            12230..13084
                     /gene="rpoH"
                     /locus_tag="ACHOTW_003515"
     CDS             12230..13084
                     /gene="rpoH"
                     /locus_tag="ACHOTW_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP9845558.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            13355..14458
                     /gene="livJ"
                     /locus_tag="ACHOTW_003516"
     CDS             13355..14458
                     /gene="livJ"
                     /locus_tag="ACHOTW_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP9845559.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            14608..14874
                     /gene="ataR"
                     /locus_tag="ACHOTW_003517"
     CDS             14608..14874
                     /gene="ataR"
                     /locus_tag="ACHOTW_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFP9845560.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            14881..15408
                     /gene="ataT"
                     /locus_tag="ACHOTW_003518"
     CDS             14881..15408
                     /gene="ataT"
                     /locus_tag="ACHOTW_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFP9845561.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(15405..15788)
                     /gene="panM"
                     /locus_tag="ACHOTW_003519"
     CDS             complement(15405..15788)
                     /gene="panM"
                     /locus_tag="ACHOTW_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP9845562.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         3926 a         4102 c         4324 g         3670 t
ORIGIN      
        1 tttaaattaa ttttctgaat aatgtttcat catttcctca aatcaaatgg ccactgttag
       61 ataaacggtg gccattgcct gctcaggagg tgctattaac ccggtaaccg acttttcggt
      121 ctgcccggcc ccagtaaaat cgccagtttg ctgccgcctt tcgtcgtttc catccagatt
      181 ttacacacgc tggttaatgg caccgaaagt agcatcccta ccgggccgag cagccatccc
      241 caaattaaca atgaaagaaa taccaccatg gtggacatcc ccaggcgatg gcccatcatc
      301 cgtggttcta aaatattgcc gatgaccata tggaccacta aaaacaatgc gccgaccaga
      361 atacattcgt aaacaccatt aaacagcagc acctgaatca ttggcggtac ggcggaaatt
      421 accgcgccga tattgggcac gtagttaagc aaaaacgcca gtactgccca catcagcgca
      481 aactgtaccc ccatcagctc cagccccagc cagacgatga cacccgtcca taaactgagt
      541 agcgtcttta acgccagata atgcgaaacg cctttaagtg cgcggtgtaa tcccgcgatg
      601 tgaatctgtg ggttattcag cgcaaaacgc attttgtaag ggacgtggcg cacttcaaac
      661 agcataaaaa ctacggtcat caccagcaaa agcacgctcg ccattgcccc ggaaagcccg
      721 gtcattagcg ctgtggtgaa ggtaaccact ttttccgagt ccatccgctg cagcattcgc
      781 tccggcgaca tatgcaaatt aagaaaaggc aacatctcct gcaatttaaa aagtttgcgc
      841 gtcagctcct tattaaactt cggcagcata gagataaatt cgttaaacga tgccgccagt
      901 acgccgacca gcgcggttag tgcgatcagc atcaccacca ctacaatcgt aatggcaacg
      961 gggcgttgta ctccccgacg aataaaccag gtgacgagcg ggttgaggac gatggcaaaa
     1021 aacagcgcca gtaacagctg cacaatgata tctgccgctg cgtgaatgcc cgcgaggatc
     1081 actaccagcg aggccagctt gagcagaatg tgcatgcccg ttttatcggg ttgaggggtt
     1141 tccattgggg cttccttgtg actttttgta ttaagtgtag cgggagtcac gacggcaata
     1201 ttctgattct tactgctgat tttcacgtca gcacgtggta aaaatgaaac actgttgtaa
     1261 aaatgtggtg atcctcatgc ccgaacccgt agccgaaccc gcgctaaacg gattgcgcct
     1321 gaatttgcgc attctctcca ttgtcatgtt taacttcgcc agctacctca ccatcgggtt
     1381 gccgctcgct gtattaccgg gctatgtcca tgatgtgatg ggctttagcg ctttctgggc
     1441 aggattggtt atcagcctgc aatatttcgc caccttgctg agccgtcctc atgccggacg
     1501 ttacgccgat ttgctgggcc ccaaaaagat tgtcgtcttc ggtttatgcg gctgcttttt
     1561 gagcggtctg ggatatctga cggcaggatt aaccgccagt ctgcccgtca tcagcctgtt
     1621 attactttgc ctgggacgcg tcatccttgg gattgggcaa agttttgccg gaacgggatc
     1681 gaccctgtgg ggtgttggcg tggttggctc gctgcatatc gggcgggtga tttcgtggaa
     1741 cgacattgtc acttacgggg cgatggcgat gggtgcgccg ttaggcgtcg tgttttatca
     1801 ctggggcggc ttgcaggcgt tagcgttaat tattatgggc gtggcgctgg tggccatttt
     1861 gttggcgatc ccgcgtccga cggtaaaagc cagtaaaggc aaaccgctgc cgtttcgcgc
     1921 ggtgcttggg cgcgtctggc tgtacggtat ggcgctggca ctggcttccg ccggatttgg
     1981 cgtcatcgcc acctttatca cgctgtttta tgacgttaaa ggttgggacg gtgcggcttt
     2041 cgcgctgacg ctgtttagct gtgcgtttgt cggtacgcgt ttgttattcc ctaacggcat
     2101 taaccgtatc ggcggcttaa acgtagcgat gatttgcttt agcgttgaga taatcggcct
     2161 gctactggtt ggcgtggcga ctatgccgtg gatggcgaaa atcggcgtct tactggcggg
     2221 ggccgggttt tcgctggtgt tcccggcatt gggtgtagtg gcggtaaaag cggttccgca
     2281 gcaaaatcag ggggcggcgc tggcaaccta caccgtattt atggatttat cgcttggcgt
     2341 gaccggacca ctggctgggc tggtgatgag ttgggcgggc gtaccggtga tttatctggc
     2401 ggcggcggga ctggtcgcaa tcgcgttatt actgacgtgg cgattaaaaa aacggcctcc
     2461 ggaacacgtc cctgaggccg cctcatcatc ttaaaactta ctgaataacc agcgtattaa
     2521 tgatgttttc tgcggtggtc tgcgcttttt gctgatcgtc agctggcaga gtaatttgca
     2581 tggtcagcag ttgattaccc acgttaccca gaataacgga agagtacgcc gtctggcctt
     2641 tcgcggagat aatactgtct aactgctgca ttttgtgacc tttcagctca atggctttat
     2701 tggtgaccac ttgcagctgt ggatcgcggc tacgttgctg atcttccaga cgcttcgcca
     2761 gcaccgccag atcttctttc ggatcatcgc ccatgatgac gatgactgct ttctgcccgg
     2821 tggcgtcgga ccagacatgc atgttattgg cctgcgttcc cagcttaccg ctctggtcgg
     2881 tcatatccgc tggcagcgag aaacttaact tgccatcaag caggttgacg ggattcccgg
     2941 tagcgttact ttccgcgacc gaaccctgcg ccgtagcgtt agtgtcttta tcatcacagg
     3001 ccgcaagccc cataaccagc aggccaattc cgacatattt aaccagattg cgcattgact
     3061 tcttcctttc gataaacggc cataacggct cattcatcca tcttatcaca actctgataa
     3121 cgaaccttta actcgcctgc aaagcgttga tttcggattt atctgccagt cttttcaaca
     3181 gcatattgag taatacgcca tacattggca ggaagaaaac gatactgatt aacactttga
     3241 aacagtaatc gaccagcgcg atttccatcc agtgttcagc cataaaggca tccgggctac
     3301 gccagaaggc aatgaagaaa aaggccagcg tgtcgctgac gttaccgaac agtgtggacg
     3361 ctgtcggtgc cagccaccag cggcgactct gacgcaggcg gttaaaaacg tgcacgtcga
     3421 ggatctgtcc cagcgcgtag gccatgaaac tggcggtggc gatacgggcg acaaacaggt
     3481 tgaagtgggc gagtgcgccg aatccctgcc atgaacccat atagaatagc gacgagatga
     3541 cgtaggagat cagcaatgcc gggatcatca ccgcaaagat gatacgacgg gccagcggtg
     3601 cgccaaaaat acgtacggtc aggtcggtag cgagaaagat aaacggaaag ctaaacgcgc
     3661 cccaggtggt atggaaacca aaaatggaga cgggaagctg aaccagatag ttactggagg
     3721 tgatcaccag cagatgaaat aacgataacc agaacaacgc cttatagcgt tgagtttgcg
     3781 agaaaacgtt catattgtac ctttttgatt aaccattggg gtgagggaac ccaatacgta
     3841 cgacacgtct gttaccttaa cataataacg tgcttaccct tttttcgagc cgccgcatga
     3901 tactgcttta cgttgacaat gcaatggtta attttcacgc aatcgttaac ctggtttgct
     3961 tacggactca cagggcgtaa actagcgccg tttttttatg tgatgagaag aaaatgaccg
     4021 atctcttttc cagccctgac cacacactcg acgcacttgg cctgcgctgc ccggaaccag
     4081 tcatgatggt gcgcaaaaca gtgcgcaata tgcagccagg tgaaacattg ctgattattg
     4141 ctgacgatcc ggcgacgacc cgcgatatcc ctgggttttg tacctttatg gaacacgaac
     4201 tggttgccaa agagacggat ggactgcctt atcgttattt gattcgtaaa ggcggttgac
     4261 gggggcagat tggcttcgat gccgcctttt cccctcaccc taaccctctc cccagaggag
     4321 cgaggggacc gatcgcgctc aatgttgcga tcggtttgcc ttatctcctg cgcaacaatc
     4381 ttaacgcatt cgctgtcacc agcaccgtcg cacccgtatc tgccagcact gccagccata
     4441 gtccggtcat ccctaacagc gtggtgacga ggaagatccc tttcagcccc agcgcaatag
     4501 tgatgttctg gcggatattg gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccagac
     4561 cgcgcaggtg gttatgggtt aatgctgcgt cggcggtttc cagcgccacg tctgtgccgc
     4621 tgcccattgc aatcccgatg gcggcagctt tcatcgctgg cgcgtcgtta ataccgtcac
     4681 cgaccatcgc cagcggcgca tgttgattca gctcggtcac cgctttgact ttatcttccg
     4741 gcaacaggcc cgctttaaac tccagcccca gctccccggc aattgccgcc gctgcgcgtg
     4801 gattatcgcc ggtgaggatc acccctttga cgcccagcgc gttcagttca ctgatggcag
     4861 ttgcagcatc ggcgcgcagg gtatcctgca atgcaatgat acccagcacg tcatcattac
     4921 gtactaccag cacaaccgtt tgcccggcgc tttccagttc attaatcaaa ccagcaaatg
     4981 catcagcggg atgtttcccg gcagcgcata tcaacacgcg ctcaccgtta acctgcgctt
     5041 caatgccaga cccgaccagc gcccgctgtg attcggcggc gggaatggcg agtgcagcaa
     5101 cctgtgcttc gcgcacgatg gcctgcgcca gtggatgcgt cgcgccttgc tcgaccgccg
     5161 ccgccagtgt cagcagttca gattcactaa tacccgttgc cggatgaatc gcggtaacgc
     5221 gcggtttacc gacggtcagc gtaccggttt tatcaaacgc cacctgggta acacgaccca
     5281 gctgttccag cgccgctccg cctttaatca acgccccacg acgcgctgcc gccgccagcc
     5341 cggaggtaat cgccgcaggc gttgagataa ctaacgcaca cgggcagcca atcagcagca
     5401 gcgtcagccc tttataaatc cactcctgcc agctggtggc aaacagcagc ggtggcacca
     5461 gcgtcaccag cagagcgacg gccataatcg cgggcgtgta aatacggctg aaacggtcga
     5521 taaaccgctc aatgggtgcg cgacgctctt cggcttcttc gatcagtttc agaatccggt
     5581 caatggcgct ggcacccggt tctgacagca cttccagcgt taccagacgg tctacgctgg
     5641 tggctcctgc aggaacttta tcgcccgttg cgcgctccac cggaatagat tcgccggtca
     5701 gggcgctttc atcaaaactg gcaaacggtg agagcagttt accgtcggca ggcaaacgtc
     5761 cacctgcggc gacttcaatc acatcgccag ggcgcaggct gttaatcgcc acctcttccc
     5821 gctcaccgtt acgcaggcgc gtggcggttt ctggtttcag cgccattaac gcgctgaccc
     5881 cttgacgcgc gcggctggcg gcccagcctt ccagtcgttc accaatcaaa aacagcagca
     5941 acaccatcgc agcttcagcc gttgcgccaa taaacagtgc accaatagcg gctacgctca
     6001 ttaaggtttc aatggcgaag tagctgccgg atttgatcag ccgtaatgcc tgacgagcaa
     6061 tcgggtacag cccaaccagc gtggtcgcga taaacgccag ttgcccgaac ggatgattaa
     6121 attgctccaa accccagctg attgccatca tcacgataag cgtaatcagc ggcagattct
     6181 ctttcaggcg cgatgcttgt ggctcgtcgg cggcctgctc atcgcgcagg gaatagcccg
     6241 ctttttgcac cgcagattca acttgtgcac ggatgtcatt gtcggcatca accaccagtt
     6301 tttcggtggc gaacaacacc tgtacctgat tcacgcctgc aagctggcgc acggcatttt
     6361 ctaccttgcg cgcacaggcg gcgcagtcca tgccgctgac tttccagcta tagcgggtgc
     6421 cggagacgtt ttcagagaga gttggcgtgc tggaacatgc gccgtcgcag caacagtcgt
     6481 tggcgttctg taccgtggtt agcggtttga acgcagcaaa ttgaggggct ttcttgccgt
     6541 gattgtcagg agtcgacatg gcatcctccg gttaagtttt ttctcattaa ccgaaggata
     6601 cactctggag tcgactccag agtcaagttt tatcagagat acagcgagcg gacgatgagg
     6661 aagtgaccgg caaagtagca ggccgcagca atcgcgttgt ccgcacggaa gcgtcggcga
     6721 tagtgactac ccagccagac aaagttactg ataaacagca acgaagcgcc gacaaacgca
     6781 gagagtgccg gagcggtcgg acggaagaac cacagttcac ctgccagcca caccatcacc
     6841 agcgtcatgc cgataaacgt gcagataggc caacgatact cttccagccg cgtccagata
     6901 atcgccagta acagcgcacc cagaaccagc aacaccagtg gcagaggcca gaagaaagag
     6961 agcgtcatct gactggcgaa atagatggtg tacagcaggt gcgagaggaa aaacgcgccg
     7021 atggcgtaca tcagacgttg acgtggcaac agggttagcg catcgcccag cagtgaggcg
     7081 cacagccctg ccagcaccag atagctaatg gcgtcgaaca tcggcgcttg ccaggccagt
     7141 aacagcagga gaagaagggt taacggttta aacacccagc gttgccaggt tggcccacga
     7201 tacgatgcat ccacagatag ccatgcggaa agacagacag cgataaacga ccaaagcatc
     7261 ttagttcctt gaattcgtta ttttttcgta agcggaatgc ctacggtctt gcttccagtt
     7321 tagtggcgag ggtggacgga tgacaacacc ataactggca gggtatgctt atttccgcat
     7381 tttccgatga gggataaaga tgagcaaacc acctcttttc tttattgtta tcattggctt
     7441 aatcatcgtc gccgcatcgt ttcgttttat gcaacagcga cgggaaaaag ctgataatga
     7501 tatggcacca ctccagcaaa agctggtgtt ggtgagcaac aagcgggaaa aaccgattaa
     7561 cgatcgccgt tcgcgccagc aggaagtgac tccggcaggc accagtatgc gctatgaggc
     7621 aagcttcaaa ccgcaaagcg gaggaatgga gcagacgttt cgcctcgacg cccagcagta
     7681 ccacgccctg acagtgggcg ataaaggtac gctgagctat aaaggaacgc gctttgttag
     7741 ctttgttggc gaacaataag gttatttttt aactttaaat ttcttctgcc agaccagcag
     7801 ttcaaacacg ccgaaaagga aaatccgcac cttttcggcg gtggtcatct gcgggccatc
     7861 tttcggtaaa gtggatttca atagcgccag ttgcatacca tgcatcagca cggtaaaaat
     7921 cagcgccacg ttaacgaaaa tattcagcgg gcgcgggaag ggatgcacca ggttgaggat
     7981 taaaaatccc caaacacaga gcattaacaa acgaccaata ttaatcagca tcactttctc
     8041 cttgtgcttc gcgttgatac agccgataag ccacctgacc cgccactttt tcccgatgta
     8101 acgaccagtt tgccggaacg gtgggcagac cgttttcgac ttcgctttcg acataaatca
     8161 gggtttcgtc agccagccag ccgttatctt ccagtaaatt tatcgtctct tctaacaaac
     8221 cacggcggaa cggtggatcg acaaacacga tattatgcgg tgtgcctttt tgcgccagga
     8281 atgacattgc gttgctgttc accacgcgtg cattgcctgc ttttagtgtc gccagattct
     8341 taattaactg ctgagaaact gcacgatcca tctcaattaa cgtggccccc gcagcgtagc
     8401 gcgataacgc ttccagcccc agcgcgccgc tcccggcgaa gcaatccaga cattgggcgt
     8461 caacaatgac cggagccagc cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc
     8521 gcagacctgg gctatcagga accgggagtt tacggcctcg ccactgcccg ccaataatgc
     8581 ggatttggcc gctgccggaa tgattcggtt ttttcatgat aaattgctca atccgccaga
     8641 taacacatac ttgcaggcgg tgatgtgaat taagttaagt gatagactat ttcatcattt
     8701 ttttagctgc tatgtacata gcgttaacgc tgtgccatga agcaacagcg aggagtgtag
     8761 tcgcaaatgg cgaaagaaaa aaaacgtggc tttttttcct ggctgggctt tggtcaaaaa
     8821 gagcagaccc cggaaaaaga gacagaagtt cagaatgaac aaacggttgt agaagaaatc
     8881 gttcaggcgc aagagcctgt gaaggcttct gaacaagccg tcgaagagca gccgcaggcg
     8941 catactgagg ccgaggcgga aacttttgct gccgacgttg tggaagtcac tgaacaggtt
     9001 gctgaaaatg aaaaagcgca gcctgaagcg gaagtcgttg cacaaccgga accggtcgta
     9061 gaagaaacgc cggagccagt ggctatcgaa cgtgaagagc tgccgttgcc ggaagacgtc
     9121 aacgccgaag aggtttcgcc agaagagtgg caggctgaag cggaaaccgt agagattgtc
     9181 gaagcggcgg aagaagaagc ggctaaagaa gaaatcaccg acgaagagct ggaagcacag
     9241 gcgctggctg ccgaagcagc agaagaggcg gtgatggtgg ttcctccggc agaagaagag
     9301 cagccggtgg aagaaatcgc tcaggagcag gaaaaaccga ccaaagaagg ttttttcgcg
     9361 cgcctgaaac gcagcctgtt aaaaaccaaa gaaaatctcg gttccggatt tatcagcctg
     9421 ttccgcggta aaaaaatcga cgatgatctg tttgaggagc tggaagagca gcttttgatc
     9481 gccgatgtgg gcgtggaaac cacacgtaaa attatcacca atctgacgga aggcgcatcc
     9541 cgcaagcagc ttcgtgatgc cgaggcgctc tatggcctgc tgaaagaaga gatgggtgag
     9601 attctggcga aagtcgatga gccgctgaat gttgagggta aaacgccgtt cgtgatcctg
     9661 atggtgggcg tcaacggtgt gggtaaaacc acgacgattg gtaagctggc acgtcagttt
     9721 gagcagcagg gtaaatcggt gatgctggcg gcgggcgata ctttccgtgc agcagcggtt
     9781 gaacagcttc aggtctgggg gcagcgcaac aatattccgg tgattgccca gcataccggg
     9841 gcggattccg cctctgttat cttcgacgcc attcaggcgg ctaaagcgcg taatatcgac
     9901 gtcctgattg ccgataccgc cggacgcctg cagaacaaat cgcacctgat ggaagagttg
     9961 aagaaaatcg tccgcgtgat gaagaaactc gacgttgaag cgccgcatga agttatgctg
    10021 actatcgatg ccagcaccgg gcagaacgcg gtaagccagg ccaaactgtt ccatgaagcc
    10081 gttggcttaa ccggcatcac gctaacgaaa ctggacggca cggcgaaagg cggggtaatt
    10141 ttctcggtgg ctgaccagtt tggtatccct atccgctaca ttggtgtcgg cgaacgtatt
    10201 gaggatttgc gtccgtttaa ggcggacgac tttatagagg cactttttgc ccgagaggat
    10261 taacaatgat tcgctttgaa catgtcagca aggcttatct cggtgggaga caggcgctgc
    10321 agggcgttac gttccatatg cagccgggtg agatggcgtt tctgaccggt cattccggcg
    10381 cagggaaaag taccctcctg aagctgatct gtgggattga gcggcccagc gccgggaaaa
    10441 tctggtttag cggccatgac atcacgcgtc tgaaaaaccg tgaagttccg tttctgcgcc
    10501 gccagattgg catgattttc caggatcacc atctactgat ggaccgtact gtctacgata
    10561 acgtggcgat cccgctgatt atcgccggtg ccagcggtga cgatattcgc cgccgggtgt
    10621 cggcggcact ggataaagtc gggctactgg acaaagcgaa gaacttccct attcagcttt
    10681 ctggcggtga acaacagcgt gttggcattg cccgcgcggt ggtgaacaag cccgcggtac
    10741 tgctggcgga cgaaccgact ggtaacttgg acgacgcgtt gtcggaaggg attctacgtc
    10801 tgtttgaaga gtttaaccgc gtaggggtaa ccgtattgat ggcaacgcac gacatcaacc
    10861 tgatttcacg gcgttcctat cgcatgctca ccctgagcga tggtcacttg catggaggcg
    10921 tgggccatga ataagcgcga tgcaatcaat catattcggc agtttggcgg gcgtcttgat
    10981 cgcttccgta aatcggtcgg cggctcaggc aacggcggtc gtaacgcacc gaaacgcgcg
    11041 aaatcctcgc caaaaccggt aaatcgcaaa accaacgttt tcaacgaaca ggtgcgctat
    11101 gccttccacg gcgcattgca ggatctgaaa agcaaaccgt tcgccacatt tttaacggtg
    11161 atggttatcg ccatctccct gacgctgccc agcgtctgtt atatggtgta caaaaacgtt
    11221 aaccaggcgg cgacgcagta ttatccgtca ccgcaaatca ctgtttatct gcaaaaaacg
    11281 ctggacgatg acgccgctgc gggcgtggtg gcacagttgc aggccgagca aggcgtagag
    11341 aaagtgaact atctttctcg tgaagacgca ctgggcgagt tccgtaactg gtctggtttt
    11401 ggtggcgcgc tggatatgct ggaagaaaac ccgcttccgg cagtggcggt ggtgatcccg
    11461 aagctcgact ttcaggggac ggagtctctg aatacgctgc gtgatcgtat cacgcagatt
    11521 aacggcattg acgaagtgcg gatggatgac agctggtttg cccgtctggc ggcgttgacc
    11581 gggctggtcg ggcgcgtttc ggcgatgatc ggcgtgttga tggtggcggc agtgttcctc
    11641 gtcatcggta acagtgtacg cctgagtatc tttgctcgtc gtgactccat taacgtgcag
    11701 aaactgattg gtgcaacgga tggtttcatc ctgcgcccgt tcctgtacgg tggcgcattg
    11761 ctggggtttt ctggcgcatt gttgtcatta attttatcag aaattctggt actgcgattg
    11821 tcatcggcgg ttgcggaagt ggcacaggtt ttcggaacga agtttgatat caatggttta
    11881 tcgttcgatg aatgcctgct gttgctgctg gtatgctcga tgattggctg ggtggcagcg
    11941 tggcttgcca cggtacaaca tttacgccac tttacgcctg aataataaaa gcgtgttata
    12001 ctctttccct gcaatgggtt ccgtagcagg gaaagagacc ccgttgtctc ttcccggtat
    12061 ttcatctcta tgtcacattt tgtgcgtaat ttattcacaa gcctgcattg aacttgtgga
    12121 taaaatcacg gtctgataaa acagtgaatg ataacctcgt tgctcttaag ctctggcaca
    12181 gttgttgcta ccactgaagc gccagaagat atcgattgag aggatttgaa tgactgacaa
    12241 aatgcaaagt ttagctttag ccccagttgg caacctggat tcctacatcc gggcagctaa
    12301 cgcgtggccg atgttgtcgg ctgacgagga gcgggcgctg gctgaaaagc tgcattacca
    12361 tggcgatctg gaagcagcta aaacgctgat cctgtctcac ctgcggtttg ttgttcatat
    12421 tgctcgtaat tatgcgggct atggcctgcc acaggcggat ttgattcagg aaggtaacat
    12481 cggcctgatg aaagcagtgc gccgttttaa cccggaagtg ggtgtgcgcc tggtctcctt
    12541 cgccgtccac tggatcaaag cagagatcca cgaatacgtt ctgcgtaact ggcgtatcgt
    12601 caaagttgcg accaccaaag cgcagcgcaa actgttcttc aacctgcgta aaaccaagca
    12661 gcgtctgggc tggtttaacc aggatgaagt cgaaatggtg gcccgtgaac tgggcgtaac
    12721 cagcaaagac gtacgtgaga tggaatcacg tatggcggca caggacatga cctttgacct
    12781 gtcttccgac gacgattccg acagccaacc gatggcaccg gtgctctatc tgcaggataa
    12841 atcatctaac tttgccgacg gcattgaaga tgataactgg gaagagcagg cagcaaaccg
    12901 tctgaccgac gcgatgcagg gtctggacga acgcagccag gacatcatcc gtgcgcgctg
    12961 gctggacgaa gacaacaagt ccacgttgca ggaactggct gaccgttacg gtgtttccgc
    13021 tgagcgtgtg cgccagctgg aaaagaacgc gatgaaaaaa ctgcgcgctg ctatagaagc
    13081 gtaatttccg ctattaagca gagaaccctg gatgcgagtc cggggttttt gttttttggg
    13141 cctctacaat aatcaatttc ccctccggca aaacgccaat ccccacgcag attgttaata
    13201 aactgtcaaa atagctattc caatatcata aaaatcgggt atgttttagc agagtatgct
    13261 gctaaagcac gggtagtcat gcataaaacg aaataaagtg ctgaacaaca acatcacaac
    13321 acacgtaata accagaagaa tggggattct caggatgaac ataaagggta aagcgttact
    13381 ggcaggatgt atcgcgctgg cattcagcaa tatggctctg gcagaagata ttaaagtcgc
    13441 ggtcgtgggc gcaatgtccg gtccggttgc gcagtacggt gaccaggagt ttaccggcgc
    13501 agagcaggcg gttgcggata tcaacgctaa aggcggcatt aaaggcaaca aactgcaaat
    13561 cgtaaaatat gacgatgcct gtgacccgaa acaggcggtt gcggtggcga acaaagtcgt
    13621 taacgacggc attaaatacg tgattggtca cctctgttct tcatcaacgc agcctgcgtc
    13681 tgacatctac gaagacgaag gcattttaat gatcacccca gcggcaaccg cgccggagct
    13741 gaccgcccgt ggctatcagc tgatcctgcg caccaccggc ctggactccg accaggggcc
    13801 gacggcggcg aaatatattc ttgagaaagt gaaaccgcag cgtattgcta tcgttcacga
    13861 caaacagcaa tacggcgaag gtctggcacg agcggtgcag gacggcctga agaaaggcaa
    13921 tgctaacgtg gtgttctttg atggtatcac cgccggggaa aaagatttct caacgctggt
    13981 ggcgcgtctg aaaaaagaga atatcgactt cgtttactac ggcggttatc acccggaaat
    14041 ggggcaaatc ctgcgtcagg cacgcgcggc agggttgaaa actcagttta tggggccgga
    14101 aggcgtggct aacgtctcgc tgtctaacat tgcgggcgaa tcagcggaag ggctgctggt
    14161 gaccaagccg aagaactacg atcaggttcc ggcgaacaaa cccattgttg atgcgatcaa
    14221 agcgaaaaaa caggacccaa gcggcgcatt cgtttggacc acctacgccg cgctgcaatc
    14281 tttgcaggcg ggcctgaatc agtctgacga tccggctgaa atcgccaaat acctgaaagc
    14341 gaactccgtg gataccgtaa tgggaccgct gacctgggat gagaaaggcg atctgaaagg
    14401 ctttgagttc ggtgtgtttg actggcacgc caacggcacg gcaaccgatg cgaagtaaca
    14461 gatagataaa tgaaaatgcc cgatgcgctg aactgccatc gggttttttt gtgtttgtcg
    14521 aaatggttgt agaaagatta aacaatccag gatatgctta atgtacggtg acataccgta
    14581 catattatac ggagcaggag agttcacatg tctgctgtta aaaagcagcg tatcgatctg
    14641 cgtttaactg acgatgacaa aagtatgatt gaggaagcgg cagcgatatc taatcagtcc
    14701 gtcagccaat ttatgttgaa cagcgcttcg cagcgggctg cggaagtgat tgaacagcat
    14761 cggcgggtga tcctcaatga ggaatcctgg acgcgggtga tggatgcgct gagtaatcca
    14821 ccgtcaccag gtgaaaagct aaaacgtgcg gcaaaacgtc ttcagggaat gtaataattt
    14881 atggatgatc tgacgataga gattctgacc gatgatgcag attatgatct acagcgattc
    14941 gactgcggcg aggaagcgtt aaatctcttt ctgacgacac atctcgttcg tcaacatcgc
    15001 aacaaaattc tgcgagcgta tatcctttgt cgcaacactc cagaacgtca ggtgctggga
    15061 tattacacat tatgcggcag ttgttttgaa cgagccgcat tgccctcgaa atcgaaacag
    15121 aaaaaaattc cctacaaaaa tattcccagc gttactcttg ggcgtctggc aattgatcgt
    15181 tcattacagg ggcagggatg gggagcaaca ctggttgctc atgccatgaa agtcgtctgg
    15241 tcagcctctt tagcggtagg tattcacggt ctttttgtcg aggcgctgaa tgaaaaagcc
    15301 catacgtttt ataaatcgct gggctttatc cctttagtcg gggaaaacga aaatgcgtta
    15361 tttttcccaa ccaaatccat tgaactgctt tttacacaga gcgattaacg cttctcccag
    15421 ccgccctgct gtgccgtaaa tcccagtgcc tgcataaacg ccgtcatcac accgcgatct
    15481 tccacgcctg catccgccat ccaccagcat gaaacgccag gattgttacg caaaacctct
    15541 tccagcagat attgccccac accgcgacgt cgagtgactt cccgcacgcg cagggaatcc
    15601 agtgccccct cggtgccgct taaggttacc cgcacggcag caagcaggcg ctcgttaaaa
    15661 cgcgcggcgt agatgcggtg gttatcgtca acctgtaaag aggaagggga atactccggc
    15721 cagatctttt gcaggtcaat ccggtcctgg tcgctaaatt tttctaatcg aatgatggtc
    15781 agcttcatgg gtaacccgtg taaatcacaa aagtggaacc agtgtagcga aataatttaa
    15841 tcggaggctt tctctttttt atttcttttg gcaggtgatt aatttcttaa cagcaataat
    15901 tacaaagtta aaacattaga gaatgaaaaa tacccagcat aatcccctga atggtagtga
    15961 attattccgc cctttgtgcc gttattttat gctgacaaag gcactttttt ctgtttatct
    16021 at
//
LOCUS       DAZKIF010000107        15867 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZKIF010000107 DAZKIF010000000
VERSION     DAZKIF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.108"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..554
                     /locus_tag="ACHOTW_003520"
                     /pseudo
     CDS             3..554
                     /locus_tag="ACHOTW_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005274.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(797..1492)
                     /gene="ykgG"
                     /locus_tag="ACHOTW_003521"
     CDS             complement(797..1492)
                     /gene="ykgG"
                     /locus_tag="ACHOTW_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP9845563.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTLLPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1485..2912)
                     /gene="ykgF"
                     /locus_tag="ACHOTW_003522"
     CDS             complement(1485..2912)
                     /gene="ykgF"
                     /locus_tag="ACHOTW_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP9845564.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2923..3642)
                     /gene="ykgE"
                     /locus_tag="ACHOTW_003523"
     CDS             complement(2923..3642)
                     /gene="ykgE"
                     /locus_tag="ACHOTW_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP9845565.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4169..5023)
                     /gene="rclR"
                     /locus_tag="ACHOTW_003524"
     CDS             complement(4169..5023)
                     /gene="rclR"
                     /locus_tag="ACHOTW_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP9845566.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5249..6574
                     /gene="rclA"
                     /locus_tag="ACHOTW_003525"
     CDS             5249..6574
                     /gene="rclA"
                     /locus_tag="ACHOTW_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP9845567.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6683..6919
                     /gene="rclB"
                     /locus_tag="ACHOTW_003526"
     CDS             6683..6919
                     /gene="rclB"
                     /locus_tag="ACHOTW_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP9845568.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6931..7524
                     /gene="rclC"
                     /locus_tag="ACHOTW_003527"
     CDS             6931..7524
                     /gene="rclC"
                     /locus_tag="ACHOTW_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP9845569.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
     gene            complement(7684..8553)
                     /locus_tag="ACHOTW_003528"
     CDS             complement(7684..8553)
                     /locus_tag="ACHOTW_003528"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9845570.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            8802..9659
                     /locus_tag="ACHOTW_003529"
     CDS             8802..9659
                     /locus_tag="ACHOTW_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9845571.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDTYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(9780..14033)
                     /gene="fdeC"
                     /locus_tag="ACHOTW_003530"
     CDS             complement(9780..14033)
                     /gene="fdeC"
                     /locus_tag="ACHOTW_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFP9845572.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSAAVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGNAKQTYKVTVTDANNNLLKDSEVTLTASPANLVLTPNGTAKTNEQGQA
                     IFTATTTVAAKYTLTAKVSQADGQESTKTAESKFVADDTNAVLTASSDVTSLVADGIS
                     TAKLEVTLMSANNPVGGNMWVDIKTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMN"
     gene            14599..15159
                     /locus_tag="ACHOTW_003531"
                     /pseudo
     CDS             14599..15159
                     /locus_tag="ACHOTW_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            15367..15723
                     /locus_tag="ACHOTW_003532"
     CDS             15367..15723
                     /locus_tag="ACHOTW_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845573.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
BASE COUNT         4174 a         3953 c         3455 g         4285 t
ORIGIN      
        1 ttctgtatat caataaagat aatataaaag tgagttacag cctaaaaata aatcagacaa
       61 cacccggcat acttgttaac tgtgatagca ataataactt tgcctgtcag acaacaatga
      121 ctgaagatgt tattcagcga attactacat tttttcacac cagcccagat gtcaagaaca
      181 gagaaataaa gctggaatgg tcaggagata agagagattt accaactgct gaagcggaaa
      241 tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc agaataccat aatggcaggt
      301 aagttctcga tgagatacaa acgccttcag caattaacag tgagctttat acaaaaatga
      361 tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta actataagct
      421 caccagaaat aaaaaataaa taccctgctg ccatttgcct ggctctggga ttttttctaa
      481 gcattgtaat ttctgtaatg ttttgtcttg tcaaaaaaat ggtagatgaa taccaacaaa
      541 actctgggca gtaataaaca gcattctgat agagagtgcc tgtataaagc tgaagtgaca
      601 atgaccggga agggaacccg ttttcaggaa tggatgcctg tctttttcac tgtaaatatc
      661 ctggcattag taatgcctgg atatttacag aagaggagca taaccatgac ggaacggggt
      721 taagtcaggt tgtccttaac gctattttca acctcgtatg ccttcttcag gtttatgtcc
      781 agacttcata cctctctcag caatcctcaa taatcagata caccgctttc accggaccat
      841 gaaccccgac gactttgata agctcaatgt ccgccgttga gctgggacca ctgatgatgt
      901 taatacatga cggcatgcgt tcaccggcct gcgctttctg atgcaatctt tctgcgagtt
      961 gcgctacacg cggcaggaga gtgcttttac gcaggataaa aagagatgat tccgggagca
     1021 ggctcaatga acgcccacgc tcggcggcgg aaaaaagaac cacgcctccc gattcggtta
     1081 aaccatattc agcatacacg acacccactt tagcctgctc tgcctgcgag atattcttgg
     1141 cacctttcgc cggatcccaa acaacggcat tgcattcctg ctgcaaacgt tcgctaatcc
     1201 ctaattcctc cagcctcgtg tcaccgctaa tcacgaccga ctgatctccc agctctttac
     1261 acagacgtat tgcagcttct gccgccttcg cctcgctggt cagctcacag cgcgtcaaca
     1321 taacatcgct ggcaaactga ataaacgcgt cacagcgctg ctgttggtta agttgggtaa
     1381 gccgctcgtt agcatagtta ttaagcggcg catcttctgc ttgcggttca agtcgcagcg
     1441 ggcgacccag tgcctgagca acgttattca aaaattcgcc tcgattatcc attctttttc
     1501 tcctgcgcct gatgtttctt aaaccaacta cggaaactct ctccgtcagc ttcaggaaga
     1561 tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt gccgccattg
     1621 ataaaccagc ttgccgcatg agcaccggcc atcatcccga ctttccacaa tcctggatga
     1681 ctattggcat aagcgaacat ttttatcgcc cgttgctctg cttttgcggt gatccctttt
     1741 tcagccatca cccgacgatg acgcaaaatc agttttgaca gcggaatacg caccggacac
     1801 acgttgtcac aagctgtgca taaagagcag gcgtagggta aatctttaaa atctttatag
     1861 ccgccaagta gcggagaaat caccgcacca attggccctg gataaataga gccatatcca
     1921 tgaccgccaa tatggcgata tgccggacaa gtattcatac aagccccgca gcgaatacag
     1981 cgcagcacat cccgaaattc agaggccagc acctcagaac gcccgttatc gacaataacc
     2041 agatgaaact cttcaggacc atcaacgtgc cccgcttcgc gaggtcctgt cagccaggtg
     2101 ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca gcatggtgat caatacatct
     2161 acctcggcaa acgtgggggc aatacgctcc attcccatca ctgcaatatg cgttttaggc
     2221 agcgtggtac acattcgcgc attaccttca ttggtcacca ggcataccga acctgtctct
     2281 gccaccgcga aattacagcc cgtaatacct atttcagcac tgaggaaatc ttcgcggatt
     2341 ttttgccgga tgaataaggt catcgcttca ggcgtttccg gcccctcata gcccagacgt
     2401 tcgtgtagca ctcgacggat ctgatggcga tctttatgaa ttgccgggac cacaacatga
     2461 gagggcggat cttgatccag ctggagaata tattcaccca aatcggtttc aatcacctga
     2521 atgccagcat cctgcaacac atgattgaca ccaatctctt cggtcaccat cgatttagat
     2581 ttcaccacct tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg ggtagcgtct
     2641 tctttggttt ttgcaaaata gacgtgaccg ccgttttgcg tcactttttc tgagagctgg
     2701 tacagataag cgtcgagatt actcagaaca tgatcacgta tctgggcggc ccgatcgcgc
     2761 cactcctccc agagccccaa ttcatcgacc attttttgcc gatttgcccc aatacgctgc
     2821 tgcgcgtttg ccaccgcttt gcgcatgatc ggatcttcaa tttgctgacg gatgcgtgtc
     2881 ttaaaatctg tattactggt tttgatcgac atctttatat cctcagcggc tcatcaacac
     2941 ttcagcaata tgcatcactt tgactttctg cccttcccgt tgtaatcgcc cactgatgtt
     3001 cagcaggcaa ctcacgtcag caccaattaa atactcaggg cggacttcca tcaggtgcgc
     3061 aaccttttct ttcaccatct cgccggatat ttcggccatt ttgaccgaga acgtgccgcc
     3121 aaatccgcag caggtatcct gttcagcaaa ggtcaacagc tccagtccac gcacattttt
     3181 cagcagcgta agtggctcgt ccttcacccc cagcttacgg gccaggctac aagatgggtg
     3241 atacaccgct ctcccttgca aactggcacc tacatcgact acccctaatt tattaacaat
     3301 aaaagaggtg agatcctgca tacgcgcggc aaccttttcg gctcgtgatg cccattcagg
     3361 ttcatccgcc agatacgtcg ggtaactttt tacggcatag gtgcaagagc cagccggtga
     3421 aataatggga tcgtcgttat cctccagtgc ggcgatcaga tttttcatcc ctggaatcgc
     3481 ttctttgata taaccactat tgatcgcagg ctgaccgcag catccctgtt tctccgggaa
     3541 atttacgcga cagccgagtt tttccagtag cagcacggag tctcgtgcca ttcttgattt
     3601 cagggcgtca ccaatacagg tgacaaagaa attgacattc acaactaata ctccattact
     3661 tcatgcccat ttattcccga tgtttccccc cacccgggga tcaatcatca ataaatatgg
     3721 cgttcttttt taattggcta gcaacatacc accccttatt tgtatgacaa atgatagtct
     3781 ttcatccaaa cttcaaatgc aataactaat tttatttctt tcggcatcgc tcacatttta
     3841 tggggaaatc gacgatcatt tttggcataa gaaatccatt taaaaatggc tacatccctt
     3901 atacctattc agagctactt attcaggggt tattgagata tacactgatg gtatacaaaa
     3961 aaattaccga caataatcac gtatcaagta atagtcggga ttttatttaa ttgaagcaat
     4021 ttagtttgca tttttattta aaaactgagc tagagtccat cagaaaaaaa tatataaagc
     4081 ccaaaccacc gttggaaata gaaatagttg aaaggctcac aactcccgga atgcggatta
     4141 tctttgccct gatttctggc ggttttattc agggtgcaag ctgtctgacc ctttcccgat
     4201 attctcccgg agtacaacca aactcgcgga caaacgcctt gtgaaaagat gattcactgg
     4261 catagcctac tgactcagcg atcaccacaa cagggagcat ttcccgggaa aacatctggg
     4321 ccgctatttg tagacgcaac tttgttaata cagccagcgg cgtggttccg gaaacatcac
     4381 ggaaaagctg ggcaaaactt gcccgggaca tgtgggcgat gctggccagc gattcgacgc
     4441 tccaggcgtg tcctggcatt tccagcattt gctgtattac cgcaccaaga cgtggatgca
     4501 gaagcaaact gagaatgttt ttctcagtat taacctgtgc aatccactca cgcaccgcga
     4561 gggtaaagaa tgtagcgcag atctggctac acagtgcatc cactcccggc attgccgatc
     4621 tggattcctg ttgtaaaaac gggatcgcct ccctcagcca gttatattcc acgctgtggt
     4681 taaccggtgc taaaaacagc gtttccggca aagacgttaa aaaataacgt gccgaatgtt
     4741 gcagccgaag agtaccacag acaatacagg tcgattcatt atcgacatga ctcaggcgat
     4801 gagctgaatt ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa atctctcccg
     4861 tcggcatttc cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata actgataatt
     4921 ccccggcacc atgtggaagc tgccagtcac ttcctaacac gcaattctta tcgatcgttc
     4981 cttgtggagc gttaagcatc aacaaacggc taagggcatc catatcagac tcctgagtgg
     5041 tttttgatta caaacaccaa caatcttagt aaacaactaa tctaatacca tcaaatagtt
     5101 aatcatgatt tttgttttgt gattctgatt aagaaaaaga aaataccaga cgataagaca
     5161 aaatatcaag cgttgtgaag aaatgttatt tgctcttttg cgtctatagt catgatgtca
     5221 aatgaacgcg tttcgacagg aaatcatcat gaataaatat caggcagtga ttattggttt
     5281 tggcaaggct ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc gagtggctct
     5341 catcgaacaa tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct gcatcccaac
     5401 caaaacattg gttcatgacg cacagcagca cacagatttt gtccgagcca tacagcgtaa
     5461 aaatgaagtg gttaattttt tacgtaataa gaattttcat aatcttgcag atatgcccaa
     5521 tatcgacgtg atcaacggcc aggcggagtt tatcaataat catagcctgc gtgttcatcg
     5581 gcctgaggga aatctggaaa ttcatggcga gaaaattttt attaataccg gtgcacaaac
     5641 cgtggttccg ccaattcctg gaattaccac cacgccagga gtatatgaca gcaccggatt
     5701 acttaatcta aaagaattgc ctgggcattt aggtattttg ggcggcggat atattggcgt
     5761 tgagttcgcc tctatgttcg ctaattttgg cagcaaggta accattttag aagcagcttc
     5821 gctgtttttg cctcgggaag aacgggatat tgctgataat atcgcgacga ttttacgcga
     5881 tcagggcgtc gatattatcc tcaatgccca tgtggagcga atcagtcacc atgaaaatca
     5941 agtgcaagtg catagcgagc acgcccaact ggcggtggat gcactgttaa tagcttccgg
     6001 tcgtcaaccg gctaccgctt cgttacatcc agaaaatgcc ggtatcgcag taaacgagcg
     6061 cggggcaatt gtcgttgaca agcgattaca taccaccgca gacaatattt gggcgatggg
     6121 agatgttacc ggcgggctgc aatttactta catatcactg gatgattacc gcattgtacg
     6181 tgatgagtta ctgggtgaag gcaaacgtag tactgatgat cggaaaaatg tgccttattc
     6241 cgtatttatg acaccgcccc tgtccagggt tggtatgaca gaagaacaag ccagagagag
     6301 tggtgctgat attcaggtgg tgacattgcc tgtagctgca attccgcgcg ccagagtgat
     6361 gaacgatacc cgtggggtat taaaagcgat tgttgataat aaaacccaac gtatattagg
     6421 ggcatcactg ctgtgtgttg actcccacga gatgattaat atagtgaaaa tggtgatgga
     6481 tgccgggctg ccttatagca tattacgcga tcagatattt actcatccgt cgatgagcga
     6541 atcactcaat gatctatttt cattagtcaa ataaactcaa aatcagacgc cagaacaaat
     6601 attctggcgt ctcagagaaa agaatcttat taattccctg ttactctata acttcttaat
     6661 cacttcattg atggtatttt atatgtttaa aaaatccgtt ttatttgcaa cactattatc
     6721 tggcgttatg gcattttcca ccaatgcaga tgataaaata attctgaaac atatcagcgt
     6781 ctcgtcagta tcagcatcac cgacagttct ggaggatgcc attgctgata tagccagaaa
     6841 atataatgct tcatcctgga aagtcacatc gatgcgaatt gataataatt caaccgcaac
     6901 agcagtattg tataaataag gatattcaca atggaaaaat acctgcacct gttaagtcgg
     6961 ggagataaaa ttggcctggc attgattcgt ctgagtattg cgattgtttt tatgtggatt
     7021 gggttattaa agtttgtccc ttacgaggca gacagcatta caccattcgt cgcaaacagt
     7081 ccactaatgt cgttctttta tgaacacccg gaagactata aacagtatct gactcacgaa
     7141 ggcgaataca aaccagaagc aagggcatgg caatcggcca ataataccta tggtttttcc
     7201 aacggtcttg gcgtcgtgga ggtgattatt gctctgctgg ttttggctaa tcctgtcaat
     7261 cgctggttag gtttattggg agggctgatg gcatttacca caccgctggt aacactctca
     7321 tttttaatca ccaccccgga ggcatgggta cccgcattgg gtgacgctca tcatggtttc
     7381 ccttatttat ccggtgctgg tcgtctggta ttgaaagata ctctgatgct ggcaggtgct
     7441 gtcatgataa tggcagattc ggcgcgggaa attcttaaac aaggcagtaa tgaatccagt
     7501 tcaacgttaa aaactgaata ttgataatcc gaacactgtc tgttgttcac tttttcaggc
     7561 gtcaggcccc ctttctgatg atgaagcctg acgccatatt accggagata tgacagagtg
     7621 aagatcaata tcactcccct gttaaaagat tcttgtcgtc tcaggtaaaa tacatgatta
     7681 atatcaatca tgtattttta cagcaagaca accacgaaca aattcaggat taaaatgttt
     7741 taccgactcc ccaacataac ccaaatcaag cgaactaatt tgtgccattt ctttctctgt
     7801 cagtgagaaa tcccagatag caaaattttc ttcgatacgc tgttgatgtg tcgatttcgg
     7861 aataacgacc actccccgtt gaatattcca acgcagaatg acttgagaaa tcgatttttg
     7921 atgggcatct gcaatactct gaagcagatt attttcaaag ggtttatgtc gtccaccacc
     7981 taacggagcc catgcttcag gctgcacgtt ataatacttc atggttgcca gcgcctctgg
     8041 ttgggcaaaa tagggatgca actcgacctg gttgaccatc ggtttaactc ttaccgtttc
     8101 acaaaagttc gccagaacat gaggatagaa attggaaacc ccaatcgctt ttaatttgcc
     8161 ctcttcatag gcatcttcga gtgcacgcca cgcactgaaa taatcgcgca tagcctgatg
     8221 caataaatag aggtcgaagt attctagtcc cgattttttt aatgatgctt caatacctgc
     8281 tgctgccgta tcctgattca acatatcctg cacccacaat ttagacgtta taaataactc
     8341 ttctcgggta cataaacctt cggatatagc ttcacgaacg gcttcaccga cggcatcttc
     8401 attaccgtat accgctgcag tgtcgatgag tcgatacccc gtgcggatag cgttcaacac
     8461 tgactgtttg cacacgtttt tatcggtaac ctgaaaaaca ccaaatccca tcatcggcat
     8521 tttcagattg ttacttaata cggaaaattc cactgtcagt ccctccatta ggcatgaaca
     8581 atgagtctac gttaaaacgt aacctcaaat tagtatgtgg attttgatat cacttatgca
     8641 aaaaattcat taataatgta ggactgaaac ctctctattt tcggggacaa cgaagcagac
     8701 gctaccagtg cttttgcctt cgcccttgct gtttttgata cacttagggc ccagggcata
     8761 acgaaaatgt gcgatatgac aaagaattaa cggagaatgt gatgatcagg cagaagattc
     8821 tacagcagct cctggagtgg attgagtgca atcttgagca ccctatttca atcgaagata
     8881 tcgcacagaa atctggctac agcagacgca acatccagct tctgttccga aatttcatgc
     8941 atgtgccttt gggagaatac attcgcaaac gaaggctttg tcgtgccgcc attcttgtcc
     9001 ggctcaccgc gaaatctatg cttgatattg caatctcttt gcattttgat tcacagcagt
     9061 cattcagccg cgaatttaaa aagttattcg gctgctctcc ccgtgtatac cgccaccgtg
     9121 attattggga tctcgcaaat atcttccctt cttttttaat acgtcaacag caaaagacgg
     9181 agtgtagatt agtcaacttt cctgagacac ctatttttgg caattcattt aaatatgata
     9241 ttgaagtgtc gaataaatca ccggatgaag aagtcaaact gcgacgtcat catttagcca
     9301 gatgtatgaa gaattttaag acggatacct atttcgtctc cacgtttgaa ccgtcaacaa
     9361 aatcggtcga tttactcaca gttgaaactt ttgccggtac ggtatgcaaa caaaccgatt
     9421 cagagatgcc gaaagagtgg acaattaacc gaggactgta tgcctctttc cgctatgaag
     9481 gtgagtggga gcactaccct gaatgggcgc gaaacctcta tctgatggaa ttacccgcca
     9541 gggggttagc cagagtgaac ggcagtgata ttgagcggtt ttattacaat gaagatttcg
     9601 tagaaaagga tagcaatgat gttgtttgcg aaatatttat ccctgttcgt ccggtttagc
     9661 tgtcgctata tctaaatgaa cttataacga gactactgtc aggcaattca ataaataatg
     9721 gcgttgaaga tgtcaacgcc attattgttt attagaatgt tacttccata ttcttaatat
     9781 tagttcatcg cctcctcgcc ctttgccgta tcggcatcca ccgcggtaat ggtcaccgtc
     9841 actggcttca tttcgtaccc gccatacgtc agggcgttaa atgtgaacgt atagacacca
     9901 ggcttgctgg tactaaatgt acgcgtgatt tttccgctca cgaaatggtc atttttcgac
     9961 ggcaggaact gataatcctt ctccgtcacc ccttctggcg tcttaatgtc gacccacata
    10021 ttccccccaa cggggttatt tgccgacatc agtgtcacct ccagcttcgc agtcgatatc
    10081 ccatccgcca ccagagaagt cacatcagat gatgcggtga gtactgcatt tgtatcatcc
    10141 gcgacgaatt tagattcggc agttttcgtc gattcctgac cgtcggcctg actcactttc
    10201 gccgtgagtg tatatttcgc tgcgacagtg gtcgtggcgg tgaaaatagc ctgtccttgc
    10261 tcattagttt tcgccgtccc attgggagtc agaactaaat ttgccgggct ggcagtcagc
    10321 gtcacttcgc tatctttcaa caggttgtta ttggcatcag tcaccgtaac tttatacgtc
    10381 tgtttggcat tgccgtccgc cagtgcatag ttgccaatca cctcaatacc ggagacggtt
    10441 gcggtagcct tatcggctac aaacgttata gtctgcgtat tcgaaggctg gctattccct
    10501 gccgatgccg tgatctcata cgttccggca gtcactgaaa ccacctgcaa ctctgctttc
    10561 ccctcatcgt tggctttcac ccagacatta tcgcctgtaa gcggcttgac accccggggc
    10621 agattaaagg tcaccaggct ccctggaaca gggttaccat ttttatcctc aacgttcgcc
    10681 gtcagcgtaa aggcatcttt gccgtttgcc actttttgag cagcggcgtc tacctgcagg
    10741 tttgcctgac cggtgctggc atccgcgttg aatttcaccg tgaccgtctt ctgagcacca
    10801 ttcaccgaag cggaaatatt gtgttctccc gcaaccgttg acataagctc aatgtccgct
    10861 ttacctgccg cattagttgt tactgtattc cccgtcttgc tggtcacacc ctgcggtaaa
    10921 gtcagcgtaa cttcctgccc ctgcaacggg ttaccatagg tgtccacaac ggtcagggtt
    10981 atctggttag cagactgtcc attagccagt tggttattaa ccttcactgt catatcacga
    11041 atctcagcct tagatgcgtc acctgcaacg ttcagcacca gtggctgagc aacggcattt
    11101 tggccgttca ctcgcggcat cacagacaac tgacccgcgg cagatcccag cgttaaggtc
    11161 gacacgtaga ccccattacc tttctctgtc cagtttcctg ctgaaggacg ctcactcccc
    11221 gtgctggcgg caccgctaaa cactggtgca tctggcttca gcccggtgac cgggttcccg
    11281 tacgcatcct tcacggtgaa ggtgagttcc gtcgtcgttt tgacggtcgg agccttattg
    11341 tccgcaacaa gcgtagagtt cgctgatgac atctctccgg cgatgaccgt caactgcgcg
    11401 gcttcggtgg ctgctggctg gccgttgaag agaggcatga cgcgaagctc gcccgtcttt
    11461 ccacctgtag tcaacgtagc aacataggaa ccgttacctt tttcggtcca actggaaacg
    11521 gtcgcccctt cagaggcggt ccccgtcaaa cttgccgaca acgcaagacc actgatagcg
    11581 ttgccatgcg catctttcgc caccagcgtc acggttgtgc tttcgccggc ttttacgtga
    11641 tcttctgcga cagagacttt cgactggttt gaagataacg catcagccac cacggttact
    11701 tttgccgcat ttgctgccgc attctgtcca tttagcttcg gcataacttc taattcaccc
    11761 gccgtagagc cgagagtaat ctgcgcagtc cacgtcccat caccattatt tgtccagccc
    11821 gatgccgtag aaccttcagc agcggcaccc gctaatgacg gcgcttccgg cgtgaggctg
    11881 gtcacagggt tgtcataggc atcttttacc gtccagattg ccgtaactgt ccccccaacc
    11941 accggtttat caggattcag ggtgatggac gaatgtgctg catcaagcgg cccggcaaca
    12001 aacttcagcg tttgttgtaa tgtgcccagc tgatactgct cgctgaaagc acgcacaaca
    12061 acgtttccgg cacgggtcga agaaaccgcg gcgctgtaaa cgcctggttt tatttccgaa
    12121 atggcaccaa cggttacacc attagtgtct tgcggaacaa atcgcaagcg gctggcttct
    12181 cccgtcaccg gattaccctc ggagtccacc gcagtcaacg tcaacgtata ggcttgctga
    12241 ccatcagcaa ccacgtcacc tgacggctcg ttagcgctca gggtggagtt tgccacatcc
    12301 atcatcgtgg cccgcagttc tgcagtgacg gttttgctca tgccatcaac gctaacagta
    12361 atcgttgcct cacctgactg cgttccggta gtaaagacag actgatacac ccctgcttca
    12421 gtttcggtga actcacccag tgttggcttt gcctgtgatt tagtggcctt cagggaacga
    12481 gtcacaatat ttccagccgg tttgaaagtt agttcagtct tgatctgatc tttcatgccc
    12541 gtgactggct ggccctcggc gtcgcgcaga gacagcacca gcggtttttg ctcattaccg
    12601 ttagcaagca tttgaatacg gctctgaccg tcaagcgtta acgccgtgcg atcggcgctc
    12661 ataccagctc cggtaatgac cacctctgtc tgcacgcgtt ttgaggtatt gcctttgtta
    12721 tcgtaggcaa ctgctgaaat cgcataataa ttgtctttgc ctggacgata agccgggagc
    12781 gttacttgcc actgactacc ctgaccggta attttgccac cttcagccag taatgacggc
    12841 gcttcccact gcacattttt cagtccgtga gttgctttgc tgaccacaag ccccagggaa
    12901 agtgtctgac cgcccttacc ttcaatacgc tcaggcagag caatacggat cacttcagat
    12961 ttgcggtact caagaacgat gttgttatta cgctcaacca ggtcatagcg gctgcctgcc
    13021 agtacccgac gctcgcgaat gctatccgta tcgagttgtt tcgccaaagg ttcgccaatt
    13081 cggtagttaa cttccaggcc aaagcgagtg tcattctcac cgctcttgcc ctgcttatgc
    13141 ccggcgctca gtgtcagaag aggcactggc gtataggtca cctcggcaga aatagcatgc
    13201 gggtctttct ggcgcttatc tttaccaaac agcccgactt catcgccata atactgttca
    13261 tacatcaggc ttgcgccaag ctgcggccag gcaggtaaat agccctctgc gcggatatcc
    13321 caaccattcg ccgggcgttc ctgataatcc tcaatatccg gcgatttttt ccagccagaa
    13381 gcccgaatat aaccattggc gctcagtttc agataatcgc gccagtattc cgcaccaaca
    13441 ccaatgcggg tatgactacg ggataaatca tggtcgataa aggtgttcac ccccgccatc
    13501 cagtcatttc ctgaaaaatg acgccagcca aaaccaatat ttgactgagt acgatcgtct
    13561 gtacgatgta ttgccccctg agtgaacaac atatttgtcg gcgtatcata aatcggataa
    13621 agcatttcca gcgaagaatc cttcagcgag aaatctttat cgacattcag tttgacgcgc
    13681 gctgtaccat atttcccgag ccactcctgt atttcctggt tagctttagc ggtggccatt
    13741 ccggtaataa aattacgtgt cgcatcgcta tctggctgac tgcttaaaaa tgtcccggca
    13801 tttgcggcaa acgacgcgac atttttctcc acgttattat cagcagttac cgtagtattt
    13861 cccatgctca accgtggctg aaccgcatgc tgcgcacgtg ccgccattac tggggtaaag
    13921 gtgacagcga gtggaaaaag aacctgaaca gagatatttg cccacgccac gcagcgggcc
    13981 agaactgaat aacgaaatcg tggttgttta tgacctgttt tataacgtga cattgagata
    14041 atttcctgat gaacgaatta acgaacatag gcagacacaa ataatggcgt ctgcttgtca
    14101 gaaaaaccgg ggatttcccg gtttcctttt ccgcagaaaa ggaaatacct ttatgtaaga
    14161 aagtacaccg ccacctaata tatccagtgc ctgtagccac tcatcaggtg gttgggcgcg
    14221 ggattatatt tttatttatg ggtgaaaatt tagctttttg tgcaagtcat taaatagata
    14281 cttaagttat atttcttact ctgcggacag tgaataattc attattcaga gtgaataata
    14341 atgacctgcc tttaattagc aactattgga aatacgtcga agtaggtata tacgccagga
    14401 taaaccgata tattttttat ggatattcat tttccttcat cggactgtaa attgcggaga
    14461 tatcgaactt gcactaccca tcgcaacact caaacagatt tatgaatttc gttcataacc
    14521 cctgacaaat cctgccgtct aatcgagctc ttctctttac gttatgcttc tcatgaacga
    14581 taacggaagg ataacaccat gcaaaaagta aaactgaata acggtattga aatgcccctg
    14641 ctgggctttg gtgtatttca gatgaccgat gccgccgaat gcgaaagagc ggtcattgac
    14701 gctattaaca ccggttatcg actgattgac accgccgcct cctatcaaaa tgaaccccag
    14761 gtcgggaacg cactgaaaca aagcggtatc gcccgtaacg aactctttgt caccacgaaa
    14821 ctctggttgc aggataccag ttacgaaggg gccaaagccc agttcgaacg ctctctgaat
    14881 cgactgcaac ttgattacgt tgacctgtat ttgattcatc agccttacgg tgatgtccac
    14941 ggtgcctggc gtgccatgga agagctgcaa caggccggta aaattcgcgc tattggcgtc
    15001 agtaacttcc atcctgaccg actggctgac cttatcgcct ttaataacgt cgttccagcg
    15061 gtaaaccagg ttgaagttaa ccctttcaat cagcagctac acgccgttcc gtggatgcaa
    15121 agccgtggta ttcagccaga agcctgggca ccgtttgcag ttacagggcg gtaagcgatt
    15181 atgcatctac ctatttacgt cggtcactca gtcagagtct tgcccatatt caatgaggtt
    15241 caacaccgca gccaatgaaa agtaggccat gcggggatta atagcattac tcattaacca
    15301 atcgctcttt tcatttacgt gctttttatg taacatattg cttttattga tcttcctgga
    15361 gtttgcatga ataagaatct catcctcgca tttgcattat tttccttacc tgtatttgca
    15421 gaagaagatc tggggccagg caaatatgtt tgtgacatta ggatttccag cctggatacc
    15481 gctactcaaa ttctgtcaaa atccgcaaca gttctcgata atggaaacaa tttcatcgtt
    15541 caaatgccaa atggagatca attgtactct ccagatctgg aaaatgttga tgatggtata
    15601 aaacaaaaag caacaatagg tggagtgaca tttattcgtc gtccaacttt taacgatcgc
    15661 ttcatcgtgg aagatggaaa tacaggattc ttctataaga tgcgaaactg cgagaagaaa
    15721 taaaggaagt ttgatagcga tatcctcctg atttcaggct ttgtacctgt tcaagactta
    15781 caactacaag ccgctacgtg ttaacgcctc ccaaaataac caccgttaac cgtagcggtc
    15841 atagatacta ttattttaac cacatta
//
LOCUS       DAZKIF010000108        15143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZKIF010000108 DAZKIF010000000
VERSION     DAZKIF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.109"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..974
                     /gene="ybeQ"
                     /locus_tag="ACHOTW_003533"
                     /pseudo
     CDS             1..974
                     /gene="ybeQ"
                     /locus_tag="ACHOTW_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(1044..1526)
                     /gene="ybeL"
                     /locus_tag="ACHOTW_003534"
     CDS             complement(1044..1526)
                     /gene="ybeL"
                     /locus_tag="ACHOTW_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP9845574.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1762..4344
                     /gene="leuS"
                     /locus_tag="ACHOTW_003535"
     CDS             1762..4344
                     /gene="leuS"
                     /locus_tag="ACHOTW_003535"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9845575.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4359..4940
                     /gene="lptE"
                     /locus_tag="ACHOTW_003536"
     CDS             4359..4940
                     /gene="lptE"
                     /locus_tag="ACHOTW_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP9845576.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4940..5971
                     /gene="holA"
                     /locus_tag="ACHOTW_003537"
     CDS             4940..5971
                     /gene="holA"
                     /locus_tag="ACHOTW_003537"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9845577.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5973..6614
                     /gene="nadD"
                     /locus_tag="ACHOTW_003538"
     CDS             5973..6614
                     /gene="nadD"
                     /locus_tag="ACHOTW_003538"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9845578.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            6638..7249
                     /gene="cobC"
                     /locus_tag="ACHOTW_003539"
     CDS             6638..7249
                     /gene="cobC"
                     /locus_tag="ACHOTW_003539"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP9845579.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7509..7826
                     /gene="rsfS"
                     /locus_tag="ACHOTW_003540"
     CDS             7509..7826
                     /gene="rsfS"
                     /locus_tag="ACHOTW_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9845580.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7830..8297
                     /gene="rlmH"
                     /locus_tag="ACHOTW_003541"
     CDS             7830..8297
                     /gene="rlmH"
                     /locus_tag="ACHOTW_003541"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9845581.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8328..10229
                     /gene="mrdA"
                     /locus_tag="ACHOTW_003542"
     CDS             8328..10229
                     /gene="mrdA"
                     /locus_tag="ACHOTW_003542"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP9845582.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10232..11344
                     /gene="mrdB"
                     /locus_tag="ACHOTW_003543"
                     /gene_synonym="rodA"
     CDS             10232..11344
                     /gene="mrdB"
                     /locus_tag="ACHOTW_003543"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP9845583.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11355..12443
                     /gene="rlpA"
                     /locus_tag="ACHOTW_003544"
     CDS             11355..12443
                     /gene="rlpA"
                     /locus_tag="ACHOTW_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP9845584.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12583..13794
                     /gene="dacA"
                     /locus_tag="ACHOTW_003545"
     CDS             12583..13794
                     /gene="dacA"
                     /locus_tag="ACHOTW_003545"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP9845585.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13904..14167
                     /gene="ybeD"
                     /locus_tag="ACHOTW_003546"
     CDS             13904..14167
                     /gene="ybeD"
                     /locus_tag="ACHOTW_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP9845586.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14268..14909
                     /gene="lipB"
                     /locus_tag="ACHOTW_003547"
     CDS             14268..14909
                     /gene="lipB"
                     /locus_tag="ACHOTW_003547"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP9845587.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3732 a         3832 c         3943 g         3636 t
ORIGIN      
        1 ttttcacgtc aagttgctgc gataatttat caatagatga gattatcgaa cgtgctgaaa
       61 aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat agcgcaattg
      121 actctccgga cgacgaaaag gccttttact agctgaagct ggccgctgag caaggtcatt
      181 gtgaagcaca gtattcctta gggcaaaagt ataccgagga taaaagccgt cataaagata
      241 atgagcaagc catcttctgg ctgaaaaaag ctgccctaca aggccatact ttcgcttcca
      301 acgcccttgg ctggatactg gatcgtggag aagaccccaa ctataaagaa gcggttgcct
      361 ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt gggtggatgt
      421 acagaaatgg caacggagcc gcacaagact atacgctggc atttttctgg tacaaacaag
      481 ctgcattaca aggccatagt gacgcgcaaa acaatctggc cgatctttat gaagacggaa
      541 aaggcgttgc tcaaaacgag acactcgccg cattctggta tttgaaaagc gcacagcagg
      601 gtaatcggca tgctcagttt caaattgcat gggattataa cgctggcgaa ggggtggacc
      661 aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcagggg agcgtcggcg
      721 cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa gattatcagg
      781 ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc tggtataacc
      841 tggccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag gctctcgacc
      901 tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt cgtgagactg
      961 aagatttact gtagagataa aaacaagggc cggggaattt cattcggccc atattctctt
     1021 tattgctgtg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa ctggtcatga
     1081 ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg gaagtgacat
     1141 ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac cccatgatga
     1201 ttgaggtcct ggaaaacttc gcgccattca agctgcgttt tatcggtgat gtctgccagc
     1261 tcctgccaca agctttcttt aatcacccgc ataaagacgc tgtcagattc ttctttcagg
     1321 ctctcttcat agctcatggc gaactcttcc aggtcacgtc tgacagctcg cgtcagctca
     1381 tcgacctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc ctgttccacc
     1441 agtgcgtcga tatcacgttc gccattgcgc aggcgttcgc tcagtgacgc aaccagttca
     1501 cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac tgactataat
     1561 aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta aattaatgta
     1621 tgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg acgcggacgt
     1681 ttgggctatg ctatgcggat ctgaaaaacc acctctaacg ctacttttgt agccgtaatg
     1741 aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat agaatccaaa
     1801 gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag
     1861 aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat gggccacgta
     1921 cgtaactaca ccatcggtga cgtgatcgcc cgttaccagc gcatgctggg caaaaacgtt
     1981 ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac
     2041 aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa
     2101 atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc ggaatactac
     2161 cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata taagaagact
     2221 tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca agttatcgac
     2281 ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca gtggtttatc
     2341 aaaatcactg cttacgctga cgagctgctc aacgatttgg ataaactgga tcactggcca
     2401 gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt ggaaatcacc
     2461 ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc ggacaccttt
     2521 atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa
     2581 aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt tgctgaagct
     2641 gaaatggcga cgatggagaa aaaaggcgtc aatactggct ttaaagctgt tcacccatta
     2701 acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta cggcacgggc
     2761 gcagtaatgg cggttccggg gcacgaccaa cgcgactacg agtttgcctc taaatacggc
     2821 ctgaacatca aaccggttat cctggcggct gacggctctg agccagatct ttctcagcaa
     2881 gccctgactg aaaaaggcgt gctgttcaac tctggcgagt tcaacggtct tgaccatgaa
     2941 gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga gcgtaaagtg
     3001 aactaccgcc tgcgtgactg gggtgtttct cgtcagcgtt actggggcgc gccgattccg
     3061 atggtgacgc tggaagacgg taccgtaatg ccgaccccgg acgaccagct gccggtgatc
     3121 ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga tccacagtgg
     3181 gcgaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt
     3241 atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga aggtatgctg
     3301 gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg tatcgaacac
     3361 gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg
     3421 gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc
     3481 ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga tgctatcgtt
     3541 gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt
     3601 tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg
     3661 gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc tccggctgat
     3721 atgactctcg aatggcagga atccggcgtg gaaggggcta accgcttcct gaaacgtgtc
     3781 tggaaactgg tgtacgagca tactgcgaaa ggtgatgttg cggcactgaa cgttgatgca
     3841 ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaactatcgc taaagtgacc
     3901 gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat ggagctgatg
     3961 aacaaactgg cgaaagcacc gaccgatggc gagcaggatc gcgctctgat gcaggaagca
     4021 ctgctggccg ttgtccgtat gcttaacccg ttcaccccgc acatctgctt cacgctgtgg
     4081 caggaactga aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa
     4141 gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt ccgtgctaaa
     4201 atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg ccaggaacat
     4261 ctggtagcaa aatatcttga tggcgttact gtacgtaaag tgatttacgt accaggtaaa
     4321 ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg gcaacattgt
     4381 tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt gacaccacgc
     4441 aggttccttc cactatgaag gtcatgatcc tggactcagg cgatccgaac gggccattaa
     4501 gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat aaagaaacca
     4561 cgcgtaagga cgttccatcc ctgcgtttgg gtgctgtatc aatctcacag gataccgcgt
     4621 cggttttccg taatggtcaa actgcggaat atcagatggt gatgactgtt agcgcgtcgg
     4681 tattgattcc tggacgagat atctacccga ttagcaccaa agtcttccgt tcattcttcg
     4741 ataacccgca aatggcgtta gcgaaagata acgagcagga aatgatcatt aaagagatgt
     4801 acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct gcggatattc
     4861 gttccgacga agaacagacg tcgactacaa cggatactcc ggcaacacct gcacgcgtct
     4921 ccaccacgct gggtaactga tgattcggtt gtacccggaa caactccgcg cgcagctcaa
     4981 tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat tgcaggaaag
     5041 ccaggacgct gttcgtcagg tagctgcggc acaaggattc gaagaacacc acactttttc
     5101 cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcca tgagtctgtt
     5161 tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaacg cggcgatcaa
     5221 tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga tcgtccgcgg
     5281 taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg cgaatcgcag
     5341 cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg ttgctgcgcg
     5401 cgcaaaacag ctcaacttag aactggatga cgcggcgaat caggtgctct gctactgtta
     5461 tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc tctggccaga
     5521 cggcaaactg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc atttcactcc
     5581 ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcattgc atattcttca
     5641 gcaactgcgt ctggaaggca gcgaaccggt tattttgttg cgcacattac aacgtgaact
     5701 cttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgtg cgttgtttga
     5761 taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa atcgcttaag
     5821 tcagccgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca ccctcaaaca
     5881 agattacagt cagtcagtgt gggcagagtt ggaagggtta tctcttctgt tgtgccataa
     5941 acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct ctgtttggcg
     6001 gcacctttga tccggtgcac tatggtcatc taaaacccgt ggaaacgctg gcgaatttga
     6061 ttggtctgac gcgagtcaca atcatcccta ataatgttcc tccgcatcgt ccccagccgg
     6121 aagcgaacag cgtacagcgt aaacacatgc ttgaactggc gattgccgac aagccattat
     6181 ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgcg caaacactga
     6241 aagagtggcg gcaggaacaa gggccggacg tgccgctggc gtttattatt ggtcaggatt
     6301 cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat gcacatttga
     6361 tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac cagcaatggc
     6421 tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc ggtaaaattt
     6481 atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa cgtttgcaaa
     6541 acggtgaatc atgtgatgat ttattgccgg aaccggtact gacttacatt aaccaacaag
     6601 gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt taattcgtca
     6661 tggcgaaaca caagcgaatg tcgatggtct gtacagcggt catgcgccca cacccctgac
     6721 cgcacgcggt attgagcaag cgcaaaatct gcatacactg ctagatgatg tttcctttga
     6781 cctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg ttctcagtga
     6841 ccgccagctc cccgttcata tcatccctga actcaacgaa atgttttttg gcgactggga
     6901 gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa
     6961 tgactggcag catgctatcc ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt
     7021 ggaacgcttt atcgcaaggc ttagtgaata tcagcactat cagaatattt taatcgtcag
     7081 ccatcagggc gtacttagtc tgttaattgc tcgtttaatt ggcatgcctg ccgagtctat
     7141 gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatataaacc aaaaattcgc
     7201 gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac ttttctgttt
     7261 ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat tctccgcagc
     7321 cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg gcgatgcaat
     7381 ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga caattgtccc
     7441 gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga ttcacccagg
     7501 gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt gatgacctca
     7561 aaggtcagga catcatcgcc ttagacgttc agggcaaatc cagcatcact gactgcatga
     7621 tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac gttgtgcagg
     7681 agtctcgcgc agcgggcctg ttaccgctcg gagtagaagg tgaaaacagc gccgactgga
     7741 ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt
     7801 atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc gtgggaacga
     7861 aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt ccgaaagata
     7921 tgcccttcga gctgattgaa attccggccg gaaaacgcgg caagaatgcg gacatcaagc
     7981 gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac cgcattgtca
     8041 ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag ctggaacgct
     8101 ggaagctgga tggtcgcgac gtcagtctac tgattggcgg gcctgaaggg ttgtcgcctg
     8161 cctgtaaagc ggcggcggag cagagctggt cgctgtcggc gcttaccctc ccccatccgc
     8221 tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc accaaccatc
     8281 cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg aaactacaga
     8341 actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct
     8401 ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat ctgcaaattg
     8461 ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg gtgcctatcg
     8521 cgcccagccg cggcattatc tacgaccgta acggtatccc tctggccctc aaccgcacta
     8581 tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg ctggacgctt
     8641 tgcgcagcgt ggttgatctg accgatgacg atattgctgc attccgaaaa gagcgcgcac
     8701 gttcacaccg tttcacctcc attccggtga aaactaacct gaccgaagta caagtagctc
     8761 gctttgccgt caatcagtac cgttttccgg gtgttgaagt taaaggctat aaacgtcgtt
     8821 actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata
     8881 aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca acgcatgata
     8941 tcggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag accggttatg
     9001 aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa gtcccaccgc
     9061 aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat attgaaacgc
     9121 tgctggcggg tagccgcgca gctgtggtag tcaccgatcc gcgtacaggt ggggtgctgg
     9181 cgctggtttc cacgcctagt tatgacccaa acttgtttgt tgacggtatc tccagcaaag
     9241 attattccgc cttgttgaac gacccgaata caccgctggt gaaccgcgcc acacaggggg
     9301 tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcattg agcgccgggg
     9361 tgatcacgcg caatacgacg ctgtttgacc ctggctggtg gcaactgcca ggttcggaaa
     9421 aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca agatcgctgg
     9481 aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc gatcgcctct
     9541 ccgaatggat gggtaaattc ggttatggtc attataccgg tatcgacctg gcggaagaac
     9601 gttccggcaa tatgcctacc cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc
     9661 agggtgacac cattccggtt ggtatcggtc agggttactg gacagcgacc ccaatccaga
     9721 tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg catttgctga
     9781 tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa ccgcccgttg
     9841 gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt gttgctaacc
     9901 gccctaacgg tacggcgcat aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat
     9961 ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg
    10021 ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac aatccgcaag
    10081 tggctgtcgc catgattctg gagaacggtg gcgcgggtcc ggcggttggt acgctgatgc
    10141 gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct gcggaaaatc
    10201 ctgcggttgc cgcagcggag gaccattaat catgacggat aatccgaata aaaaaacatt
    10261 ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat tgctggttta
    10321 cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat
    10381 cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt
    10441 ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg tggcggtaga
    10501 tgctttcggt gccatctcta aaggcgctca acgctggctg gacctcggta ttgttcgttt
    10561 tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct ttatcaaccg
    10621 cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga tatttatgcc
    10681 cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg cgctttccgg
    10741 tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg cagtagtgct
    10801 ggtagcggcg ttcattccga ttctgtggtt cttcctgatg catgattacc agcgccagcg
    10861 cgtaatgatg ctcctggacc cggaatcaga cccactcggc gcgggctatc acattattca
    10921 gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc acggcactca
    10981 gtcacagctt gaatttctcc ccgaacgcca tactgacttt atcttcgcgg tactggcgga
    11041 agagctggga ttagtgggca ttttgattct gctcgctctc tacattctgc tgatcatgcg
    11101 cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg ctggcggctt
    11161 aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg gtattctgcc
    11221 ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa ttgtgctgat
    11281 ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt
    11341 gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca ggaatgctcg
    11401 cggcatgtac aagcgatgat ggtcagcaac agacagtaag tgtaccgcag cctgcggtat
    11461 gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca ctgaacgcga
    11521 cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag gatccgtctc
    11581 gatttatcca ggcgggactg gcggcaatct atgatgccga accaggcagt aacctgacgg
    11641 cctctggcga agctttcgat ccgacacagc tgacggcggc ccatccaacg cttccgatcc
    11701 ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg cgcattaatg
    11761 atcgcggtcc ttacggcaac gaccgcgtta tttcgctttc tcgcgcagca gctgaccgtc
    11821 ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc caggatggtt
    11881 cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac gccctgcctg
    11941 cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg cagggtgaca
    12001 ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca
    12061 gcagcggttt cctcggcgca ccaacgacct tagcgccagg tgtactggaa ggcagcgaac
    12121 cgacgcctgc tccacagccc gttgttacag ctccgtcgac aacgcctgca acctcgcctg
    12181 caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg gtgcaggtcg
    12241 gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga cagaagttcg
    12301 gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt ggcccattcg
    12361 ccaacaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc caattacagt
    12421 catttattac caccgcgcag tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat
    12481 gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt gctatagtag
    12541 ggcacttttt ttaattccat cacggatgtc gtagttcaga ccatgaatac cattttttcc
    12601 gctcgtatca tgaagcgcct ggcgctcacc acggctcttt gcacagcctt tatctctgct
    12661 gcacatgccg atgacctgaa tatcaaaact atgatcccgg gtgtaccgca gatcgatgcg
    12721 gagtcctaca tcctgattga ctataactcc ggcaaagtgc tcgccgaaca gaacgcagat
    12781 gtccgccgcg atcctgccag cctgaccaaa atgatgacca gttacgttat cggccaggca
    12841 atgaaagccg gtaaatttaa agaaactgat ttagtcacta ttggcaacga cgcatgggcc
    12901 accggtaacc cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg catgcaggtt
    12961 ccggtttctc agctgatccg cggtataaac ctgcaatcgg gtaacgatgc ttgtgtcgcc
    13021 atggccgatt ttgccgctgg tagccaggac gcttttgttg gcttgatgaa cagctacgtt
    13081 aacgccctgg gcctgaaaaa tacccacttc cagacggtac atggtctgga tgctgatggt
    13141 cagtacagct ccgcgcgcga tatggcgctg atcggccagg cgttgatccg tgacgtaccg
    13201 aatgaatact cgatctataa agaaaaagaa tttacgttta acggtattcg ccagctgaac
    13261 cgtaacggcc tgttatggga taacagcctg aatgtcgacg gcatcaaaac cggacacact
    13321 gacaaagcag gttacaacct tgttgcttct gcgactgaag gccagatgcg cttgatttct
    13381 gcggtgatgg gcggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa actgctaacc
    13441 tggggcttcc gtttcttcga aaccgttaac ccactgaaag taggtaaaga gttcgcctct
    13501 gaaccggttt ggtttggtga ttctgatcgc gcttcgttag gggttgataa agacgtgtac
    13561 ctgaccattc cgcgtggtcg catgaaagat ctgaaagcca gctatgtgct gaacagcagt
    13621 gaattgcatg cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt ccagcttgat
    13681 ggcaaaacga tcgaacaacg cccgctggtt gtgttgcaag aaatcccgga aggtaacttc
    13741 ttcggcaaaa tcattgatta cattaaatta atgttccatc actggtttgg ttaaaaattg
    13801 aacacttgaa agtgtaattt ccgtccccat atactaagca tcagtaaaaa actcccgcct
    13861 tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa ccaaacttaa
    13921 cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg cgttacctga
    13981 gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact acaccccaac
    14041 ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca acgccactca
    14101 tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg tccgcatggt
    14161 tctgtaactc gcttctccgt tacccggccc ctggtcgggt aactcccctc tcctcgctgt
    14221 gatatacttt tctcccactt ttactcattc tccacggaga tgccgttttg tatcaggata
    14281 aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccag gctatgcatg
    14341 aattcaccga tacccgcgat gatagtaccc ttgatgaaat ctggctggtc gagcactatc
    14401 cggtattcac ccaagggcag gcaggaaagg cggagcacat tttaatgccc ggtgatattc
    14461 cggtgattca gagcgatcgc ggtgggcagg tgacttatca cgggccgggg caacaggtga
    14521 tgtatgtatt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg gtgaccttgc
    14581 ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat cctcgggctg
    14641 acgcgccagg tgtctatgtt ggggaaaaga aaatttgctc actgggttta cgtattcgac
    14701 gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca ccatttttac
    14761 gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa tggaaacccg
    14821 aagcgacgac taataatatt gcaccacgtt tactggaaaa tattttagcg ctactaaaca
    14881 atcctgactt cgaatatatt accgcttaat ttcatacctc aatggcccaa tttacactgg
    14941 gtcattactc ataacacctg ctaatgcttt acatctcctt cgcgtttgta tattttcttt
    15001 atcacttgca gaaatgcgat gctacgcctt gctccgtaac tcaccccatt gattaactta
    15061 ttgaaatagt tgattatgat gagcaaaagc gttccgttcc aattagacat aagattattg
    15121 taattttttt aaatattatt ttt
//
LOCUS       DAZKIF010000109        14998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZKIF010000109 DAZKIF010000000
VERSION     DAZKIF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.110"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            140..1198
                     /gene="citC"
                     /locus_tag="ACHOTW_003548"
     CDS             140..1198
                     /gene="citC"
                     /locus_tag="ACHOTW_003548"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP9845588.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTESFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1213..1509
                     /gene="citD"
                     /locus_tag="ACHOTW_003549"
     CDS             1213..1509
                     /gene="citD"
                     /locus_tag="ACHOTW_003549"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP9845589.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1506..2414
                     /gene="citE"
                     /locus_tag="ACHOTW_003550"
     CDS             1506..2414
                     /gene="citE"
                     /locus_tag="ACHOTW_003550"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP9845590.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     ESVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2425..3957
                     /gene="citF"
                     /locus_tag="ACHOTW_003551"
     CDS             2425..3957
                     /gene="citF"
                     /locus_tag="ACHOTW_003551"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP9845591.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3961..4512
                     /gene="citX"
                     /locus_tag="ACHOTW_003552"
     CDS             3961..4512
                     /gene="citX"
                     /locus_tag="ACHOTW_003552"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP9845592.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4487..5365
                     /gene="citG"
                     /locus_tag="ACHOTW_003553"
     CDS             4487..5365
                     /gene="citG"
                     /locus_tag="ACHOTW_003553"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP9845593.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5416..6879
                     /gene="citT"
                     /locus_tag="ACHOTW_003554"
     CDS             5416..6879
                     /gene="citT"
                     /locus_tag="ACHOTW_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP9845594.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6993..7799
                     /gene="rna"
                     /locus_tag="ACHOTW_003555"
     CDS             6993..7799
                     /gene="rna"
                     /locus_tag="ACHOTW_003555"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP9845595.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8029..8439
                     /gene="rnk"
                     /locus_tag="ACHOTW_003556"
     CDS             8029..8439
                     /gene="rnk"
                     /locus_tag="ACHOTW_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP9845596.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8577..8666
                     /gene="yldA"
                     /locus_tag="ACHOTW_003557"
     CDS             8577..8666
                     /gene="yldA"
                     /locus_tag="ACHOTW_003557"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP9845597.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8670..9908)
                     /gene="ybdR"
                     /locus_tag="ACHOTW_003558"
     CDS             complement(8670..9908)
                     /gene="ybdR"
                     /locus_tag="ACHOTW_003558"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFP9845598.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10129..10557
                     /gene="uspG"
                     /locus_tag="ACHOTW_003559"
     CDS             10129..10557
                     /gene="uspG"
                     /locus_tag="ACHOTW_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP9845599.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10678..12243)
                     /gene="ahpF"
                     /locus_tag="ACHOTW_003560"
     CDS             complement(10678..12243)
                     /gene="ahpF"
                     /locus_tag="ACHOTW_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9845600.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12372..12935)
                     /gene="ahpC"
                     /locus_tag="ACHOTW_003561"
     CDS             complement(12372..12935)
                     /gene="ahpC"
                     /locus_tag="ACHOTW_003561"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9845601.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13307..14053
                     /gene="dsbG"
                     /locus_tag="ACHOTW_003562"
     CDS             13307..14053
                     /gene="dsbG"
                     /locus_tag="ACHOTW_003562"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP9845602.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14263..>14998
                     /locus_tag="ACHOTW_003563"
     CDS             14263..>14998
                     /locus_tag="ACHOTW_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9845603.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLG"
BASE COUNT         3645 a         3908 c         3708 g         3737 t
ORIGIN      
        1 tgtatagcga aaggagaaaa agatatacct cgatcacccc cctttctccc aagtgaaaat
       61 aaaaggttat cagtttgcaa cattgaacaa cattcgttgc aaatcgataa caacatgcac
      121 cttcaggata ctatttatta tgttcggcaa tgatattttc acccgcgtaa aacgttcaga
      181 aaataaaaaa atggcggaaa tcgcccaatt cctgcatgaa aatgatttga gcgttgacac
      241 cacagtcgaa gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat
      301 tgccggaaat attattaaat gcgttgctat cagtgaatcc gttcgcggtg aaggactggc
      361 gctgacatta gccactgaac tgataaacct cgcctatgag cggcacagca cgcatctgtt
      421 tatttatacc aaaaccgaat acgaggcgct gttccgccag tgcggttttt ccacgctgac
      481 cagcgtaccc ggcgtgatgg tgctgatgga aaacagcgcc acgcgactga aacgctatgc
      541 cgaatcgctg aaaaaatttc gtcatccagg gaacaagatt ggctgcattg cgatgaacgc
      601 caatcccttt acgaatggtc accgttatct gattcaacaa gctgcggcac agtgcgactg
      661 gttgcatctg tttttagtca aagaagattc ttcacgcttc ccctatgaag accggctgga
      721 tctggtgtta aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg gctccgaata
      781 catcatctcc cgcgctacgt tcccttgcta cttcattaaa gaacagagcg tcattaacca
      841 ttgttacacc gaaattgatc tgaagatttt ccgtcagtac ctcgctcccg cgctgggtgt
      901 aactcaccgc tttgtcggta ctgaatcctt ttgtcgcgtt accgcccagt acaaccagga
      961 tatgcgctac tggctggaaa cgccgactat ctccgcagcg cccatcgaac tggttgaaat
     1021 tgagcggctg cgttaccagg agatgccgat atccgcttcc cgggtacgtc aactgctggc
     1081 gaaaaacgat ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc attatttgca
     1141 gaacctgctt gagcactccc gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga
     1201 aacaggtgaa aaatgaaaat aaaccagccc gccgttgcag gcacccttga gtctggggat
     1261 gtgatgatac gcatcgcccc actcgatacg caggatatcg acctgcaaat caatagcagc
     1321 gttgagaaac agtttggcga tgcaattcgc accaccattc tggacgttct cgcccgctac
     1381 aacgtgcgcg gcgtacagct gaatgtcgat gataaaggcg cactggactg cattttacgt
     1441 gcacgactgg aagccctgct ggcacgcgcc agcggtatcc cggctctgcc atgggaggat
     1501 tgccaatgat ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt
     1561 ttgtgcctgg tgccaatgcc gcgatggtca gcaactcctt catctacccg gctgatgccc
     1621 tgatgtttga cctcgaagag tccgtggcat tacgtgaaaa agacaccgcc cgccgcatgg
     1681 tttatcacgc gctgcaacat ccgctgtatc gcgatattga aaccattgtg cgtgtaaacg
     1741 cgctggattc cgaatggggt gttaacgacc tggaagccgt cgttcgcggt ggtgcggacg
     1801 ttgtacgtct gccgaaaacc gataccgctc aggatgttct ggatatcgaa aaagagatcc
     1861 tgcgtataga aaaagcctgt ggtcgtgaac ccggcagcac cggcctgctg gcggcgattg
     1921 aatctccgct gggcattacc cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg
     1981 gtatcgccct cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc tccccggaag
     2041 gaactgaact gctgttcgca cgctgttcca ttttgcaggc cgcgcgctct gcgggtattc
     2101 aggcgttcga taccgtctat tccgacgcta acaacgaagc cggattcctg caagaagccg
     2161 cccacatcaa acagctgggc tttgacggca aatcgctgat caacccgcgt cagattgatc
     2221 tgctgcacaa cctctacgca ccgacccaga aagaagtgga tcacgcccgc cgcgtcgtag
     2281 aagccgctga agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg
     2341 tggacggtcc ggttatcgat cgtgcccgtc tggtgctctc ccgtgcagaa ctttccggca
     2401 tccgcgaaga ataaggcaat caaaatgacg cagaaaattg aacaatctca acgacaagaa
     2461 cgggtagcgg cctggaatcg tcgcgctgaa tgcgatcttg ccgctttcca gaactcacca
     2521 aagcaaacct accaggctga aaaagcgcgc gatcgcaaac tgtgcgccaa cctggaagaa
     2581 gcgattcgtc gctctggttt acaggacggc atgacggttt ccttccatca cgctttccgt
     2641 ggcggtgacc tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac
     2701 ctgatcctgg cgtccagctc cctgagtgat tgccatgcgc cgctggtaga acacattcgc
     2761 cagggcgtgg ttacccgcat ttatacctcc ggcctgcgtg gtccactggc ggaagagatc
     2821 tcccgtggtc tgctggcgga accggtgcaa atccactctc acggcggacg tgtgcatctg
     2881 gtacagagcg gcgaactgaa tatcgacgtg gctttcctcg gcgtcccgtc ctgtgatgaa
     2941 ttcggtaatg ccaacggcta caccggtaaa gcctgctgcg gctccctcgg ctatgcaatg
     3001 gttgatgccg acaacgcaaa acaggttgtg atgcttaccg aagaactgct gccttatccg
     3061 cataatccgg caagcattga gcaagatcag gttgatttaa tcgtcaaagt tgatcgcgtt
     3121 ggcgatgctg caaaaatcgg cgctggtgct acccgtatga ccactaaccc gcgcgaactg
     3181 cttattgccc gtagcgctgc ggatgtgatt gtcaactcag gctacttcaa agaaggtttc
     3241 tccatgcaaa ccggcaccgg cggcgcatcg ctggcggtaa cccttttcct ggaagacaaa
     3301 atgcgtagcc gcgatattcg cgccgacttc gccctcggtg gtattaccgc gacgatggtt
     3361 gatctgcacg aaaaaggtct gatccgcaaa ctgctggatg tacagagctt tgacagccac
     3421 gctgcgcaat cgctggcccg taaccccaat cacatcgaaa tcagcgccaa ccagtacgct
     3481 aactggggtt cgaaaggtgc atcggttgat cgtctcgacg tggtggtact gagcgcgctg
     3541 gaaattgaca cccagttcaa cgttaacgtg ctgaccggct ctgacggcgt actgcgtggt
     3601 gcttccggtg gtcactgcga taccgcgatt gcctctgcgc tttccatcat cgtcgcgccg
     3661 ctggtacgcg gtcgtattcc gactctggtg gataacgtgc tgacctgcat caccccgggc
     3721 tccagtgtcg atattctggt cacagaccac ggtatcgcag ttaacccggc acgtccggaa
     3781 ctggcagaac gtctgcagga agcgggcatt aaagtggttt ccattgagtg gctgcgcgaa
     3841 cgtgcgcgtc tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg cgtcgttgcc
     3901 gttgtgcgtt accgcgatgg ctcggtgatc gatgttgtgc atcaggtgaa ggaataagcc
     3961 atgcacctgc ttcctgaact cgccagccac catgcggtgt caattcccga gctgctcgtc
     4021 agccgggatg aaaggcaagc acggcaacac gtctggctca agcgccatcc tgttccactg
     4081 gtctccttta ccgtggttgc gcctgggccg attaaagaca gtgaggtcac acgccgaatt
     4141 tttaatcatg gcgtgacagc cttgcgtgcc ttagccgcaa aacagggctg gcaaattcag
     4201 gagcaggctg cactggtttc cgccagcggg ccggagggta tgttgagcat tgccgccccg
     4261 gctcgcgacc tcaagctcgc caccattgag cttgaacata gtcatcctct cgggcggtta
     4321 tgggatatcg atgtcctgac gcccgaaggc gaaattctct cccgccgcga ctattcactg
     4381 ccgcctcgcc gctgcctgtt gtgcgagcaa agcgcagccg tctgcgcgcg tggaaaaacc
     4441 catcaactga ccgatttact caaccgcatg gaggcactgc tgaacgatgt cgatgcctgc
     4501 aacgttaact aaaaccacaa agcttgcgac gtcattaatc gatgagtacg ccctgctggg
     4561 ctggcgcgcc atgctgactg aagtcaatct gtcaccgaaa ccaggtctcg tggatcgcat
     4621 taactgcggt gcgcataaag atatggcgct ggaagatttc caccgcagcg cgctggcgat
     4681 tcagggctgg ctaccccgtt tcattgaatt tggtgcctgt agtgcggaaa tggcaccaga
     4741 agcggtactc cacggattac gcccaattgg tatggcttgc gaaggtgata tgttccgcgc
     4801 caccgcgggc gtaaacacgc ataagggcag cattttttct ttagggctac tgtgtgcggc
     4861 aattggccgt ttgcttcagc tcaaccagtt gctaacgcca acaaccgttt gttctacggc
     4921 ggcaagtttc tgccgtggcc tgaccgatcg cgaactgcgt accaataatt cacaactgac
     4981 ggcaggtcaa cggttgtacc agcagcttgg ccttaccggc gcacgcggtg aagccgaagc
     5041 gggttatcca ctggtgatca accacgcctt gccgcattac ctcactctgc tggatcaggg
     5101 gttagatcct gaactggcat tgctcgatac cttgctccta ctgatggcga tcaacggcga
     5161 taccaacgtt gcttcgcgcg gtggcgaggg gggcctgcgc tggctacagc gcgaggcgca
     5221 aacattattg caaaaagggg gcattcgaac ccccgcagat ctcgattatc tccggcagtt
     5281 cgacagggag tgtatcgaac gaaatctcag tccaggcggc agtgccgacc tgctgatcct
     5341 tacctggttt ttagcacaga tttaattatt taagaacttg ataaatttgg aaatattaat
     5401 tttcggagaa cccgtatgtc tttagcaaaa gataatatat ggaaactatt ggccccactg
     5461 gtggtgatgg gtgtcatgtt tcttatccct gtccccgacg gtatgccgcc gcaggcatgg
     5521 cattacttcg ctgtgtttgt ggcaatgatt gtcggcatga tcctcgagcc aattccggca
     5581 acagcgatca gttttattgc ggttactatt tgcgttattg gcagtaatta cctgctcttt
     5641 gatgccaaag aattagctga cccagcgttt aatgcgcaaa aacaggcgct gaaatggggc
     5701 ctggctggtt tttccagcac tacggtatgg ctggtatttg gcgcatttat ttttgcatta
     5761 gggtatgaag tttccgggtt aggtcgtcgc attgcccttt tcctggtgaa attcatgggc
     5821 aaacgcacgc tgacgttggg ttatgcgatt gtcattatcg acattctgct ggcaccgttt
     5881 acaccgtcca acaccgcgcg taccgggggt acggtttttc cggtcattaa aaacctgccg
     5941 ccgttgttta aatcattccc gaacgatccg tccgcgcgtc gtattggcgg ctatttgatg
     6001 tggatgatgg tcattagtac cagtctgagt tcgtccatgt ttgtcaccgg tgcggcacca
     6061 aacgtgctgg gtctggagtt cgtcagcaaa attgccggta tccagattag ctggttgcag
     6121 tggttcctct gcttcctgcc ggttggggtt atcttgctta tcattgcgcc gtggctttcc
     6181 tacgtgctgt acaaaccgga aatcacacac agtgaagaag tggcaacctg ggcgggtgat
     6241 gaactaaaaa ccatgggtgc gctgacacgc agagagtgga cgctgattgg ccttgtattg
     6301 ctcagcttag gtttgtgggt atttggcagt gaagtcatta atgctactgc ggttggtctg
     6361 ctggcagttt cgctaatgct ggctctgcac gttgtgccgt ggaaagacat tacccgctat
     6421 aacagcgcat ggaacacgct ggtcaacctg gcaactctgg ttgtgatggc taacggcctg
     6481 actcgttctg gttttattga ctggttcgcc ggtaccatga gtacgcacct ggaaggattc
     6541 tcaccaaacg caacggtgat tgtactggtt ctggtgttct actttgcaca ctacctgttt
     6601 gccagcctgt ctgcgcacac cgcaaccatg ctgccggtta ttctggccgt cggtaaaggt
     6661 attccgggcg taccaatgga acaactgtgt atcctgctgg tgctgtctat cggtatcatg
     6721 ggctgtctga cgccgtatgc aaccggtcct ggggtgatta tttacggctg tggctatgtg
     6781 aaatcaaaag attactggcg tcttggcgca atcttcgggg tgatttacat ctctatgttg
     6841 ctgttggttg gctggccgat tctcgccatg tggaactaat acgttcgcca aacgctgaat
     6901 aaatcatcac gcccgccagg ttagcccacc tggcgggctt ttttatgatt taatagatag
     6961 tattcagtgt atgtatgagt tccacaccca ttatgaaagc attctggcgt aacgccgcgt
     7021 tgctcgcggt ttctctgctt cccttctctt ctgccaacgc cgtagcgttg caggcaaaac
     7081 agtatggcga ttttgatcgc tatgtcctgg ccctctcctg gcaaaccgga ttttgccaga
     7141 gtcaacacga tcgaaatcgt aacgaacgag atgaatgtcg cctgcaaacc gaaacgacca
     7201 acaaagctga ttttctgacc gtacatggtc tgtggccagg attgcctaaa tcggttgctg
     7261 cccgtggtgt tgatgaacgc cgctggatgc gcttcggttg cgctactcgc ccaatcccga
     7321 atctaccaga agcgcgcgcc agccggatgt gttcatcgcc ggaaaccgga ttatcactgg
     7381 aaacggccgc taaactaagt gaagtcatgc caggagctgg cggacgttcc tgcctggaac
     7441 gctacgaata tgccaaacac ggtgcctgct ttggttttga tccggacgca tacttcggta
     7501 cgatggtacg cctgaatcaa gagataaaag agagcgaagc cggaaaattc cttgcggata
     7561 attacggtaa aacagtgagc cgccgtgact ttgacgccgc ctttgccaaa agctggggaa
     7621 aagagaacgt gaaagcagtt aagctaacgt gccagggtaa ccctgcgtat ttgactgaaa
     7681 ttcaggtctc gatcaaagct gacgccatca acgctccgct ttctgcaaac tcattcttgc
     7741 cacaaccaca cccaggtaac tgtggcaaaa cctttgtgat tgataaagcg ggttattaat
     7801 cctctgattt acaaaacacg ctaattcgtc tttcataaac catgacgatt aagaattata
     7861 cataatgcgc tgcattgagg aattccctga atgtgacgca aatcacttca aagagtgagc
     7921 aacagtcagt atcatcatgt gagttaaacc ctcgccgcct gacggtgagg gttttctttt
     7981 gggtatgttt atctgcgaca ctcgcagtac cgacgacatg gagtaaaaat gtccagacca
     8041 actatcatca ttaacgacct ggatgccgaa cgcatcgata ttctgctgga gcaacccgcc
     8101 tatgctggtt tgccaatcgc cgacgcgtta aacgcagagt tggatcgcgc ccaaatgtgt
     8161 tcgccagaag agatgccaca cgacgtggtg acaatgaaca gccgggttaa attccgcaat
     8221 cttagcgatg gcgaagtgcg tgtgcgcacg ctggtgtatc cggcaaaaat gaccgatagc
     8281 aatactcagc tttccgttat ggctccggta ggtgccgcac tgctgggact gcgcgttggc
     8341 gattccattc actgggaact tccgggcggc gttgcaaccc accttgaagt gctggaactc
     8401 gaataccagc cagaagctgc tggcgactac ctgctttaat cccatctgta caagcgataa
     8461 ctcgctttgc ccagaggatg cactcgcatc ctctttatgc aaaatcagca atttttcctg
     8521 gttctcactg ttcacttttg ctgcaaaatt attccgtttc gtttgtggga ataaccatgg
     8581 cagaagcatt ttacatcctg ataggatttc tgattatggc ggcgattatc gtcatggctg
     8641 tcctttacct cgaaaaccac tcctgacgct catattgttc cccccggcat cgcattcacc
     8701 agacctgaaa ccgccttctg cgccgcctct gcgctttgtg caccgggtac cagaatcacc
     8761 ttacggcact cctcttcacg cttttcaaag atctcatatc cccgggcggc ctcttcaaac
     8821 ggcatatagt gggtaacaat ttcttctggt ttcagtaatc ctttctcaat taacggtaat
     8881 aattctccca gccatgcgtg aacgtgggtc tgtcccattt taaacgtcaa ccctttatca
     8941 aaggcgtcgc caaacaggaa accgtgaata aatccagcgt agacgcccgg tacgctaaca
     9001 atgccgccac gcctgaccgc cgcaatacac tgacgcaacg ctttaccgct gctgccctcc
     9061 agtttcaggt tagtcagcac cgtttccgtg gtgctgcctt tcgcttcaaa accgacggcg
     9121 tctattactg catccacgcc ccggtgacct gccgtttgtt caataattga ctgtgccgga
     9181 tcgctatctt catcgaaatt aatcgggatc gcgccgtagc ggtcagcggc gaaacgcaag
     9241 cggtagggat gatgatcaac aacaaaaatc tgttccgcac cgagcaaccg tgcacaggcg
     9301 attgtcaaca atcccacagg accggcacca tagacagcaa cgcttgaacc ttgttggatc
     9361 tgcgcatttt ttgctgcctg ccatgccgtt ggcagaatat cagaaaggaa aagggcttta
     9421 tcatctgaaa gcaaaggcgg tactttaaac ggccccacat tccctttagg gacacggaca
     9481 tactccgcct gtccaccagg aacgccacca tacaggtgac tataaccaaa caatgccgcg
     9541 ggagctggta tctgtttttt attgagcgca gcgcctttac ccgcattggt attttcgcag
     9601 gcggcatatt gctgcagtcg acagaaaaaa cagtcgccac aagcaatgac gaacggaatt
     9661 accacccggt cgcctttttg caaatttttt acatcctttc cggtttcaac tacttccccc
     9721 ataaattcat gaccaaaaat atcgccatgt ttaacctggg gtattttgcc tcgataaaga
     9781 tggaggtcag agccacagat cgccgttgcc gttatacgca gaataatatc atctgcctgt
     9841 tcaatgcccg gatcgggaac attttctacc tgaacgtgat gtgggccgtg ataagtcaat
     9901 gctttcatgc aacctccaga gacagttcaa aaaagagtca gtctttaagg gtagctatca
     9961 cagcgggaaa acgggcggta ccgcaggttt tgggagaatt cctggtgttc agtgagaata
    10021 tattcatcac agaaacagtt cgtaaggaaa aagaaccaga caaatatcag ggataaatat
    10081 tataatcatg gcgaatgatt tgttcatgat taacagggag aaagggttat gtataagaca
    10141 atcattatgc cagttgatgt atttgaaatg gaattgagcg acaaagctgt tcgccacgct
    10201 gaattcctcg cccaggatga cggagttatt catctacttc acgtactacc cgggtcagcc
    10261 agcctgagcc tgcaccgttt tgccgctgat gtgcgtcgtt ttgaagagca tctgcaacat
    10321 gaagcagaag aacgtttgca aacgatggtc agccacttca ccatcgatcc ttcccgcatt
    10381 aaacaacatg tccgttttgg tagcgtgcgg gatgaagtca atgagttagc agaagaactg
    10441 ggggctgatg ttgtagttat tggttctcgc aacccatcga tttcgaccca tctgttaggt
    10501 tctaacgcct cgagcgtgat ccgccacgcc aatctgcctg tactggtcgt tcgctaacag
    10561 ataaaagtat ttatgccgcc ctgcctggaa catcccggca gggcttttta atgccataaa
    10621 aaagccgcca ggtttgaccc cggcggctaa gcaattgcag gtgaatctta cttcttctta
    10681 tgcagttttg gtgcgaatca ggtagtcaaa agcactcaga gaggctttgg caccttcgcc
    10741 agtggcgatg atgatctgct tgtacggaac cgtcgtacag tcacccgctg cgaacacgcc
    10801 tttcacgttg gtttcgcatt tcgcatcaat gataatctcg cccatgcggt tacgttcgac
    10861 tgcgccttcg agccagttgg tgttcggcag cagaccaatc tgcacgaaaa taccggccag
    10921 ttcgatgttg tgaatatcgc cgctgacacg atctcgatat tccagaccaa cgactttgct
    10981 gccgtcgcct ttcacttccg tggtttgcgc attcagaata atgtcgacgt ttttcaggct
    11041 gcgcagtttg tcctgcagaa cctggtcggc tttcatttct ggcgcaaatt ccagcagcgt
    11101 tacgtgctca acgatacccg ccaggtcaat tgccgcttcc acgccggagt taccgccgcc
    11161 gataaccgct acgcgtttac ctttaaacag cgggccgtcg cagtgcgggc agtaggtcac
    11221 gcctttggtg cgatactgat cttcgcccgg aacgttcatg ttgcgccatt ttgcaccggt
    11281 cgccacgata atgctgcgtg ctttcagtac cgcgccagaa gctgtttcaa tctgatgcag
    11341 accaccttca actgctgctg ggatcagttt gctggcgctc tggctgtcga tcacatcaac
    11401 gtcgtattca tcaacgtgaa ctttcagtgc gcctgccagc ttctgccctt cagtcttcgg
    11461 tacagaaatg tagttttcga tatcaacggt atcgaggatc tgaccaccaa aacgttcgcc
    11521 catcagaccg gtacggatgc ctttacgtgc ggagtaaatt gctgccgctg cacccgccgg
    11581 gccggaaccg acgattaata cgtcataagc atcacgcttg ttcagctctt ctgccgcacg
    11641 tttttccgcg cccgtatcaa ttttggcaac gatttcagtc aaagtcatgc ggccctgacc
    11701 aaactctttc ccgttcacga acactgccgg aacgcccatc acgttacgat cagtgatctc
    11761 gttctggaag gtgccgccgt caattgcagt gtgcttgatg cgcgggttca atacgctcat
    11821 caggttcagc gcctgtacca cgtccgggca gttatggcaa gagagcgagt aataggtttc
    11881 gaattcaaaa tcaccgtcaa tatggcgaat ctgctccagc agagactgcg cttctttcga
    11941 cggatgacca ccggtccaca gtaacgccag taccagcgag gtgaactcgt ggcccagcgg
    12001 ggagcctgca aaacgtggcc cctggttaga acctgggttg gtgatcagga aagacggctt
    12061 acgcaccggc aagctgttat cttctttaaa ggtgactttg tctgacagtt ctgcgatttc
    12121 agccaacagt tccttgattt ctgccgattt agcgctgtca tccagcgtgg caattaactc
    12181 aacaggcttg gtcaatttct caaggtaagc cttgagttga gttttcatat ttgtgtcgag
    12241 catgtttatc tcctgggctt taaacatcat catgcaagcg gcctctgcgg ctacctggat
    12301 gcagcttgca tcatggtgcg gggaagaaaa tcgggtgccg cagcacccga ttaattacgc
    12361 gattgcggaa cttagatttt accaaccagg tccagagacg gagccagagt tgcttcacct
    12421 tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg tgctgctttg
    12481 attttacgca gcaggtcaga cgcgtcacgg ccaatgcctt cagcggtaac ttcgattgcc
    12541 tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc ttcatcttca
    12601 cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat cgcatatttg
    12661 attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg agtatcggta
    12721 gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc agcaacgtca
    12781 cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa gacgctccag
    12841 cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa tgcctggttt
    12901 ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt gttttcgatg
    12961 agatgtaagg taaccaattt ttaccgattc ggctaatgcg tttccattat caaatcgata
    13021 agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc agcggcgata
    13081 aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct ttggtatctt
    13141 cagcgccagt ctgcaggctg cgttgttgct cttacaaatg caacaacgac atggattaca
    13201 acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga caatgatagg
    13261 ttatgccttt tactcgactt ttgcactgac tgaaaaggac aaattaatgt taaaaaagat
    13321 acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctg ctccagtaaa
    13381 agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg gaggaatgaa
    13441 aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc cagatggtaa
    13501 gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta acacacttat
    13561 cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg aacaatccca
    13621 ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttcg ccgatccgtt
    13681 ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt ctggcaaagt
    13741 acagttgaga acattgttgg ttggggttat caaaccagaa agtccggcga cagccgcggc
    13801 aattcttgcc tccaaagatc ccgcaaaaac ctggcaagaa tatgaagcct ctggtggcaa
    13861 gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt taagcgacaa
    13921 cgagaaactc atggacgatc tgggggcaaa tgtcactccg gctatctatt acatgagtaa
    13981 ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta atatcattat
    14041 gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt ttcaaccatc
    14101 ctaaaaaagc atgaattgct cgctctgccc taagtggctg tgctatttta gaaaaactta
    14161 atcctgagtt aagcaaaaaa aacataatca ataaaaatat atacgcaaaa aacattgatt
    14221 aagtgaatat atcatggaag aaaaatataa ccggagtagt gtatgaccaa tctctacgac
    14281 ttgaaaaagt tcgacttaaa tcttcttgtc atatttgagt gtatttacca gcatttaagc
    14341 attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca gtcgttacaa
    14401 cgcttacgtg cgcaatttaa tgacccttta ttcattcgct caggaaaagg tatcgcgccg
    14461 acgacaacag ggttaaatct gcatcaccat cttgagaaaa atctcagagg ccttgaacaa
    14521 acaattaata tagtcaataa gtccgaacta aagaaaaact ttattattca cggccctcag
    14581 ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga ttcctctgtc
    14641 gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact gttggttcac
    14701 cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt catttgcatg
    14761 ccattgcata cgattcgcaa tactctaatt tgtagtaata ggcatccacg aattactgac
    14821 aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatatc taaatcagca
    14881 ggggttgatg atattcaaat ggaaatagac gagagattta tgaatcgcaa aatttcattt
    14941 cgtggttcct ctttgctgac aatcattaat agcattgcag ttacagattt actgggaa
//
LOCUS       DAZKIF010000110        14985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZKIF010000110 DAZKIF010000000
VERSION     DAZKIF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.111"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(187..1218)
                     /gene="rsmC"
                     /locus_tag="ACHOTW_003564"
     CDS             complement(187..1218)
                     /gene="rsmC"
                     /locus_tag="ACHOTW_003564"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP9845604.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1321..1734
                     /gene="holD"
                     /locus_tag="ACHOTW_003565"
     CDS             1321..1734
                     /gene="holD"
                     /locus_tag="ACHOTW_003565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP9845605.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1703..2149
                     /gene="rimI"
                     /locus_tag="ACHOTW_003566"
     CDS             1703..2149
                     /gene="rimI"
                     /locus_tag="ACHOTW_003566"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9845606.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2164..2841
                     /gene="yjjG"
                     /locus_tag="ACHOTW_003567"
     CDS             2164..2841
                     /gene="yjjG"
                     /locus_tag="ACHOTW_003567"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP9845607.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            2932..4521
                     /gene="prfC"
                     /locus_tag="ACHOTW_003568"
     CDS             2932..4521
                     /gene="prfC"
                     /locus_tag="ACHOTW_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9845608.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4914..5519
                     /gene="osmY"
                     /locus_tag="ACHOTW_003569"
     CDS             4914..5519
                     /gene="osmY"
                     /locus_tag="ACHOTW_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP9845609.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5646..5807
                     /locus_tag="ACHOTW_003570"
     CDS             5646..5807
                     /locus_tag="ACHOTW_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP9845610.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            5929..7002
                     /locus_tag="ACHOTW_003571"
     CDS             5929..7002
                     /locus_tag="ACHOTW_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP9845611.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            6999..7781
                     /locus_tag="ACHOTW_003572"
     CDS             6999..7781
                     /locus_tag="ACHOTW_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9845612.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(7995..8858)
                     /locus_tag="ACHOTW_003573"
     CDS             complement(7995..8858)
                     /locus_tag="ACHOTW_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP9845613.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(8830..10379)
                     /locus_tag="ACHOTW_003574"
                     /pseudo
     CDS             complement(8830..10379)
                     /locus_tag="ACHOTW_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            10637..11416
                     /gene="deoC"
                     /locus_tag="ACHOTW_003575"
     CDS             10637..11416
                     /gene="deoC"
                     /locus_tag="ACHOTW_003575"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9845614.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11543..12865
                     /gene="deoA"
                     /locus_tag="ACHOTW_003576"
     CDS             11543..12865
                     /gene="deoA"
                     /locus_tag="ACHOTW_003576"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP9845615.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            12917..14140
                     /gene="deoB"
                     /locus_tag="ACHOTW_003577"
     CDS             12917..14140
                     /gene="deoB"
                     /locus_tag="ACHOTW_003577"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9845616.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14197..14916
                     /gene="deoD"
                     /locus_tag="ACHOTW_003578"
     CDS             14197..14916
                     /gene="deoD"
                     /locus_tag="ACHOTW_003578"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9845617.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3549 a         3918 c         4064 g         3454 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc ctgatgtgta attgctggaa aaatgtccat caggagacta
      121 acgtgcaggt tttgtaagtc ggataaggcg ttcacgccgc atccgacacg gtattcggcg
      181 agataattaa cctttcttcg cctggcgggt cataatggcg cgatacacct tgaagcgccc
      241 tgtttgcgcg atcacttcgt ggaagccaaa tgtctcatcc agcacgtccg gataaggcag
      301 gaaggcgttc gctacaattc gcagctcgcc gccgctatta agatgacgca ccgcaccgcg
      361 aatcagcgtt tgcgccgcat ccaggctggt ttgcatccca tcgtggaacg gcgggttgga
      421 gatgatcata tcaaaacgac ctttcacctc ggaaaagacg ttgctggcaa agacttcacc
      481 ttcaacaccg ttggccgcaa gtgttgcgcg gctggcttct accgctggcg cagagacatc
      541 gcacaaggtg agacgaattt tcggcgaatg gcgcgcaaag gcaaccgaaa gcacgcccgc
      601 gccacagccg acatccagca ctttaccttt cgtgtgcggg gttaacgtcg agagcagcaa
      661 ctggctaccg acatccagac cgtcgcggct aaacacgcca ggcagcgttt tgaccgtcag
      721 gccatcgacg ctgtattcgc cccagaattt atcggcatca aataccggct gtttttccag
      781 acgaccaaaa tagaggccac agcgacgggc gctgtcgact ttattcaacg gcgcataatc
      841 agccagcatc tgctcggcgc tgcgcacgcc gctgcggttc tcgccaacaa caaaaatatc
      901 cgtccccacc ggaagcagag aaagtaaatt catcaactgg aactgggctt ccggtttgtt
      961 cttcggccag tagtaaatca gcgtatcgca atctgcgaca tcatccgccg tggcgaccag
     1021 actaaaacgg gcgttatccc ccatctggcg gcttaacacc tgccaatgct ggaattgctg
     1081 ggtatgagca cggctggccg cggtatctaa acgcgcgggc aggtcatcct gtaagtctcc
     1141 ggcaaacaga atacggcttt gttcgaaatc atcactgtga cgcagcaaga cttcacttgc
     1201 cggggtaaat gcagacatgg aatgctcctc aattgatact ggcggcgatt atagccatat
     1261 gttggcgcgg tatcgacgaa tttgctatat ttgcgcccct gacaacagga gcgattcgct
     1321 atgacatccc gacgagactg gcagttacag caactgggca ttacccagtg gtcgctgcgt
     1381 cgccctggcg cgttgcaggg ggagattgcc attgcgatcc cggcacacgt ccgtctggtg
     1441 atggtggcaa acgatcttcc cgccctgact gatcctttag tgagcgatgt tctgcgcgca
     1501 ttaaccgtca gccccgatca agtgctgcaa ctgacgccag aaaaaatcgc gatgctgccg
     1561 caaggcagtc gctgcaacag ctggcggttg ggtactgacg aaccgctatc actggaaggc
     1621 gctcaggtgg catcaccggc gctcaccgaa ttacgggcaa acccaacggc acgcgccgcg
     1681 ttatggcaac aaatttgcac atatgaacac gatttcttcc ctcgaaacga ctgatttacc
     1741 agcggcttac cacattgaac aacgcgccca cgcctttccg tggagtgaaa aaacctttgc
     1801 cagcaaccag ggcgagcgtt atctcaactt tcagttaacg caaaacggca aaatggcggc
     1861 gtttgcgatt acgcaagtgg tgctggatga agctacattg ttcaatattg tggtcgatcc
     1921 tgactatcag cgtcagggat tgggaagggc gctgctggaa catctgatcg acgaactgga
     1981 aaaacgcggc gtggcgacac tatggctgga agtccgtgcc tcaaatgctg ccgccattgc
     2041 cctgtacgaa agtttaggct ttaacgaggc gacgattcgc cgcaattact accccaccac
     2101 ggacggtcgc gaagacgcca tcatcatggc gttgccaatc agtatgtaag acaaggtgga
     2161 ataatgaagt gggactggat tttctttgat gccgatgaaa cgctgtttac ctttgactca
     2221 ttcaccggcc tgcagcggat gtttcttgat tacagcgtca cctttaccgc tgaagatttt
     2281 caggactatc aggccgttaa caagccactg tgggtggatt atcaaaacgg cgcgatcact
     2341 tcattacagc ttcagcacgg gcggtttgag agctgggccg aacggctgaa cgtcgagcca
     2401 ggtaaactca acgaagcctt tattaatgcg atggcggaaa tctgcacgcc gctgccgggc
     2461 gcggtttctc tgcttaacgc cattcgtggc aacgccaaaa tcggcatcat caccaacggc
     2521 tttagtgcct tgcaacaggt gcgtctggaa cgcacgggcc tgcgtgatta cttcgatttg
     2581 ctggtgattt ccgaagaagt tggcgttgcc aaaccgaata agaaaatttt cgattatgcg
     2641 ctggaacagg cgggcaatcc tgaccgttca cgcgtgctga tggttggcga cactgccgag
     2701 tccgatattc tcggtggcat caacgccggg cttgcgacct gctggctgaa tgcacaccat
     2761 cgcgagcaac cagaaggcat cgcgcccacc tggaccgttt cttcgttgca cgaactggag
     2821 cagctcctgt gtaaacactg attgcctccc ccccgttgat gggtaaaata gccgcaattt
     2881 ttcgttttca acaagcgcgg cgcgatgccg cttactcaag aagaaagaat tatgacgttg
     2941 tctccttatt tgcaagaggt ggcgaagcgc cgcacttttg ccattatttc tcacccggac
     3001 gccggtaaga ctaccatcac cgagaaggtg ctgctgttcg gacaggccat tcagaccgcc
     3061 ggtacagtaa aaggccgtgg ttccaaccag cacgctaagt cggactggat ggagatggaa
     3121 aagcagcgtg ggatctccat tactacgtct gtgatgcagt ttccgtatca cgattgcctg
     3181 gttaacctgc tcgacacccc ggggcacgaa gacttctcgg aagataccta tcgtaccctg
     3241 acggcggtgg actgctgcct gatggttatc gacgccgcaa aaggtgttga agatcgtacc
     3301 cgtaagctga tggaagttac ccgtctgcgc gacacgccga tcctcacctt tatgaacaaa
     3361 cttgaccgtg atatccgcga cccgatggaa ttgctcgatg aagttgagaa cgagctgaaa
     3421 attggctgcg caccgatcac ctggccaatt ggctgcggca agctgtttaa aggcgtttac
     3481 cacctttata aagatgaaac ctatctctat cagagcggta aagggcacac cattcaggaa
     3541 gtccgcattg ttaaagggct gaataacccg gatctcgatg ctgcggttgg tgaagatctg
     3601 gcacagcagc tgcgtgacga actggaactg gtgaaaggcg cgtctaacga gttcgacaaa
     3661 gagctgttcc ttgcgggcga aatcactccg gtattcttcg gtactgcgct gggtaacttc
     3721 ggcgttgatc atatgctgga tggcctggtg gagtgggcgc ctgcgccgat gccgcgtcag
     3781 accgataccc gtaccgtaga agcgagcgaa gataaattta ccggcttcgt atttaaaatt
     3841 caggccaaca tggacccgaa acaccgcgac cgcgtggcgt ttatgcgtgt ggtgtccggt
     3901 aaatatgaaa aaggcatgaa gctgcgccag gtgcgcactg cgaaagatgt ggtgatctcc
     3961 gacgcgctga cctttatggc gggtgaccgt tcgcacgttg aagaagcgta tccgggcgat
     4021 atcctcggcc tgcacaacca cggcaccatt cagatcggcg acacctttac ccagggtgag
     4081 atgatgaagt tcaccggtat tccgaacttc gcaccagaac tgttccgtcg tatccgcctg
     4141 aaagatccgc tgaagcaaaa acagctgctc aaagggctgg tacagctttc cgaagagggc
     4201 gcggtgcagg tgttccgtcc aatctccaac aacgatctga tcgttggtgc ggttggtgtg
     4261 ctgcagtttg atgtggtggt agcgcgcctg aagagcgaat acaacgttga agcagtgtat
     4321 gagtcagtca acgttgccac tgcccgctgg gtagaatgtg cagacgcgaa gaaattcgaa
     4381 gagttcaagc gtaagaacga aagccaactg gcgcttgatg gcggcgataa cctcgcttac
     4441 atcgctacca gcatggtcaa cctgcgcctg gcacaggaac gttatccgga cgttcagttc
     4501 caccagaccc gcgagcatta attcttgcct ccagggcgcg gtagccgctg cgtcctgtca
     4561 atttcccttc cttattagcc gcttacggaa tgttcttaaa acattcactt ttgcttatgt
     4621 tttcgctgat atcccgagcg gtttcaaaat tgtgatctat atttaacaaa gtgatgacat
     4681 ttctgacggc gttaaatacc gttcaatgcg tagatatcag tatctaaagc cgtcgattgt
     4741 cattctaccg atattaataa ctgattcaga ggctgtaatg gtcgttattc atcactcatc
     4801 gcttttgtga tggcgaccat tgacttctgt agagggtgaa gtctctccct attcagcaat
     4861 gcaacctcgt gttgccaggc tcaaattacg agcaaacata caggaataaa tcgatgacta
     4921 tgacaagact gaagatttcg aaaactctgc tggctgtaat gttgacctct gccgtcgcga
     4981 ccggctctgc ctacgcggaa aacaacgcgc agactaccaa tgaaagcgca gggcaaaaag
     5041 tcgatagctc tatgaataaa gtcggtaatt tcatggatga cagcgccatc accgcgaaag
     5101 tgaaggcggc gctggtggat catgacaaca tcaagagcac cgatatctct gtcaaaaccg
     5161 atcaaaaagt cgtgaccctg agcggtttcg ttgaaagcca ggcccaggcc gaagaggcag
     5221 tgaaagtggc gaaaggtgtt gaaggggtga cctctgtcag cgacaaactg cacgttcgcg
     5281 acgctaaaga aggctctgtg aagggctacg cgggtgacac cgccaccacc agtgaaatca
     5341 aagccaaatt gctggcggac gatatcgtcc cttcccgtca tgtgaaagtt gaaaccaccg
     5401 atggcgtggt tcagctctct ggtatcgtag attctcaggc acaaagtgac cgtgctgaaa
     5461 gtatcgccaa agcggtagat ggtgtgaaaa gcgttaaaaa tgatctgaaa actaagtaat
     5521 tcgtcgtaat tcgtcctccc gaactttgtc gggaggcgcg atgtgcacca cactaaaaat
     5581 atcgcattga gtgattttca cgctcatatc aagcggtgac attaactatg gtaaaggaga
     5641 cgcttatgtt tcgttggggc atcatatttc tggttatcgc gttaatcgcc gccgcacttg
     5701 ggtttggtgg tctggccggt accgctgcag gcgcagctaa aattgtcttt gtcgtcggga
     5761 ttattctgtt cctggtgagt ttgttcatgg gccgaaaacg accctagatt tcagaccatt
     5821 aagaaaattt tatccaaagc cagtccagcg gactggcttt tgcggtttta gcgaataata
     5881 aattgcgtta ctttgtcatt ctttcacaac aaaaacagga aggcagaggt ggggcagcga
     5941 atacctgtaa cgcttggtaa tattgcgccg ttgtcgctaa ggccgttcca gcctggacga
     6001 atagctctgg tgtgcgaagg cggcggacag cgtggaattt tcacggctgg cgtgctggat
     6061 gagtttatgc gcgcgcagtt taatcctttc gatctttatc tcggcacatc tgccggggcg
     6121 cagaacctct cggcgtatat ctgcaatcag cccggttacg cgcgcaaagt catcatgcgc
     6181 tataccacaa aacgcgaatt tttcgatcca ttgcgctttg tccgtggagg aaatcttatc
     6241 gatctcgact ggctggtgga ggccaccgca agccagatgc cgttgcaaat ggataccgcc
     6301 gcgcggttgt ttgacagcgg caaatcgttt tatatgtgcg cctgtcgtca ggatgactac
     6361 gcgccgaatt actttttacc aaccaaacaa aactggctgg atgtgattcg cgcctccagt
     6421 gcgatacctg gcttttatcg tagcggagtg tcgctggaag gcattaacta cctggatggc
     6481 gggatcagtg atgcgattcc ggttaaagag gcggcaaggc agggcgctaa aacgttggtc
     6541 gtcatccgca ctgtgccgtc acaaatgtac tacacgccgc agtggttcaa acgcatggaa
     6601 cgctggctgg gtgacagtag cctgcagccg ctggtcaatc tggtgcagca tcatgaaacc
     6661 agctatcgtg acattcagca atttattgag aaaccaccgg gcaagctgcg gatattcgaa
     6721 atttatccgc cgaagccatt acatagtatc gcgcttggca gtcggattcc ggcgctgcgt
     6781 gaagactata aacttgggcg tttatgcggt cgttatttcc tcgccacggt tggcaagcta
     6841 ttaactgaaa aagcgccgct tacccgccat ctggtgccag tggtgacgcc ggaatcgatt
     6901 gtcattccgc ctgcgccagt cgccaacgat acgctggttg ccgaagtgag cgacgctccg
     6961 caggcgaacg acccgacatt taacaatgag gatctggctt gatttgccgt tttatcgaca
     7021 cccactgcca ttttgatttc ccgcctttta ttggcgatga agaggccagc ctgcaacgcg
     7081 cggcacaagc gggcgtaggc aagatcattg ttccggcaac cgaggcggaa aattttgccc
     7141 gcgtgcaggc gttagcggaa aattatcaac cgctgtatgc cgcattgggc ttgcatcctg
     7201 gtatgttgga aaaacatagc gatgtgtctc ttgatcagct acagcaggcg ctggaaaggc
     7261 gtccggcgaa ggtggtggcg gtgggggaga gcggtctgga tctcttcggc gacgatccgc
     7321 aatttgagag gcagcagtgg tttctcgacg aacaactgaa actggcgaaa cgctacgatc
     7381 tgccggtgat cctgcattca cggcgcacac acgacaaact ggcgatgcat cttaaacgcc
     7441 acgatttatc gcgcactggc gtggttcacg gtttttccgg cagcctgcaa caggccgagc
     7501 ggtttgtaca gctgggctac aaaatcggcg taggcggtac tatcacctat ccacgcgcca
     7561 gtaaaacccg cgttgtcatc gcaaaattac cgctggcatc gttgttgctg gaaaccgacg
     7621 cgccggatat gccgctcaac ggttttcagg ggcagcctaa ccgcccggaa caggctgcgc
     7681 gggtgtttga cgtgctttgc gagttgcgcc cggaaccaga agatgagatt gccgaagtgt
     7741 tgcttaataa cacgtatgcg gtgtttaacg ttcgtgggta gtttgccctt cgtaggcagc
     7801 atccggcagt cggcgcataa tgcctgatgc gacgcttaac gcgtcttatc atgcctacag
     7861 gtttgtgccg aaccgtaggc cggataaggc gttcacgccg catccggcag ttggcgcaca
     7921 atgcctgatg cgacgcttga cgcgtcttat caggcctaca agtctgtgcc gaaccgtagg
     7981 tcgtatccgg catgtcacaa atacagcgcc ggaaatatta accggctcac cccgcgctcc
     8041 cgtaacgcat cagccaacgg ctcaacgtct tccggcgtgg cgctcgccca gctttgcgcc
     8101 tcgccataca cgccgtgggc atgaaacgcg ttcaggcgta ccggaacatc gccgactccc
     8161 ttgataaacg ccgccagttc ttcgatgtgt tgcaaataat ccacttggcc agggattacc
     8221 agcaaacgca gttccgccag cttaccgcgc tctgccagca aacagatgct gcgcttaatc
     8281 tgctgattat cgcgtccggt taattgctga tgacattcgc tcccccacgc tttgagatcg
     8341 agcattgcgc cgtcgcacac cgggagcaat ttttcccagc cggtttcgct caacataccg
     8401 ttactgtcca ccaggcaggt gaggtggcgc agttgcggat cgtttttgat agcagtaaac
     8461 agcgccacta caaacggcag ttgggtcgtg gcttcgccgc cactcaccgt tatcccttcg
     8521 ataaacagca ctgctttgcg gacatggcta agcacctcat ccacgctcat ggattgcgcc
     8581 attggcgtgg catgttgcgg acacctcttc aggcaggtat cacattgctc gcagaccaca
     8641 gcgttccaca ccactttgcc gtcaacaatc tgcaacgcct gatgcggaca ctgtggcacg
     8701 cactccccac agtcattgca acgtcccatc gtccacggat tgtgacagtt tttgcagcgc
     8761 agattgcagc cctgcaaaaa cagagccaga cgactgcctg gcccgtcaac gcaggagaag
     8821 gggataatct tactgactaa agcgcatctg ctgttcatgg cttatcacgc gcggctggcg
     8881 ttccagaata cgagtgttgc gtgcggcttc ttcgcccagc caggtggtgt tggtgcgtga
     8941 accttcggcg cgatattttt ctaaatccga caaacgcacc atataaccgg taacgcgaac
     9001 cagatcgtta ccgctgacat tggcggtaaa ttcacgcatt ccggctttaa aggcaccgag
     9061 gcaaagctgt accaacgcct gcgggttacg tttgatggtt tcgtcgagcg tcagaatgtc
     9121 gctgatgcca gaataataat aagcatgatg cgggcgacag tttgcagatg ggtgatcgga
     9181 tctggctcat cgccatacgg caaacgcgcg cccggcgtgg tgccgatatc ggaactgatc
     9241 cccgactgtg cgtgtaacat ggcgcgtttt tgccagccat atttcacggg ggtattggcg
     9301 acaaactccg ccagctgtgc actgatgcga taacctactt catttgcggc ggcttcttta
     9361 ccgtagcgcg cggcaatccc ttctttttca cacagcaagt taaccgcttc cgccagccca
     9421 tacatgccaa acattggcac aaaacgttca gggttaatca gcccttcttt caccaggaag
     9481 ctattctcaa agaagtgtga ttgttgatag aggaattcac accgcgcatc gatgatggcg
     9541 atctgctgct ggcagtagtg cggtagagtg cgcgtaaaga agtcatccag cgattcgctg
     9601 cgctcggcaa tggctttcag gttaaggcgt accagcgtgc tgccaccacc cgccagcggc
     9661 agtgagttgt aacagctcac aatcccgtag cccccttttg tgaaaatttt atcatgcacc
     9721 ggaccgttgg cgatgtgcgg tttgctacat tcacagatgt tcttcgccac ttccagcagc
     9781 aggtcatcag gggtgatttc aggatcgtag ataaaggtca ggttcggtga aacctgcttc
     9841 aaatctgcgt ctgcacgtaa gatcgcgcgg gtaatgggcg aatcagacgg gccgatattg
     9901 gcgtgcataa aggcgtctgg cagggttctg tcgaggtaac gccagaaacg ttttattcga
     9961 acatcgatct cgtcttgtgt tagaattata acatacggtt gcaacaacgc atccagttgc
    10021 cccaggtaga ccggcatcga tgtgaccgac ggtacgtggt ggtaaagaat ggtcagcaga
    10081 gagagtgcgt catcaagatc tttcgcgcct tccagctcca gccattcgga accgttcgcc
    10141 agaaaacggg cgtaatcggg taagacatag cgcggtttgt acggcgcatg accttcaaac
    10201 atatcgcaga ttacaccttc atccagcgcg cggcgggctt cggcaggaag ctgtgggtaa
    10261 ggcagattgt tttctgcttc cagtgccaga aaatggcgct tctgctccgg gctaagcact
    10321 gggctggtga caatttgctg gcaacgttgt tgcagtgcat tttcatgaga agtgggcatc
    10381 ttcttttcct tttatgccga aggtgatgcg ccattgtaag aagtttcgtg atgttcactt
    10441 tgatcctgat gcgtttgcca ccactgacgc attcatttga aagtgaatta tttgaaccag
    10501 atcgcattat agtgatgcaa acttgtaagt agatttcctt aattgtgatg tatatcgaag
    10561 tgtgttgcgg agtagatgtt agaatactaa caaactcgca aggtgaattt tattggcgac
    10621 aagccaggag aatgaaatga ctgatctgaa agcaagcagc ctgcgtgcac tgaaattgat
    10681 ggacctgacc accctgaatg acgacgacac cgacgagaaa gtaattgctc tgtgtcatca
    10741 ggccaaaact ccggtcggca ataccgccgc tatctgtatc tatcctcgct ttatcccgat
    10801 tgctcgcaaa acactgaaag agcagggcac cccggaaatc cgtattgcta cggtaaccaa
    10861 cttcccacac ggtaacgacg acatcgaaat cgcgctggca gaaacccgtg cggcaatcgc
    10921 ctacggtgct gatgaagttg acgttgtgtt cccgtaccgc gcgctgatgg cgggtaacga
    10981 gcaggttggt tttgacctgg tgaaagcctg taaagaggct tgcgcggcag cgaatgtact
    11041 gctgaaagtg atcatcgaaa ccggcgaact gaaagacgaa gcgctgatcc gtaaagcgtc
    11101 tgaaatctcc atcaaagcgg gtgcggactt catcaaaacc tctaccggta aagtggctgt
    11161 gaacgcgacg ccggaaagcg cgcgcatcat gatggaagtg atccgtgata tgggcgtaga
    11221 aaaaaccgtt ggtttcaaac cggcgggcgg cgtgcgtact gcggaagatg cgcagaaata
    11281 tctcgccatc gcagatgaac tgttcggtgc tgactgggct gatgcgcgtc actaccgctt
    11341 tggtgcttcc agcctgctgg caagcctgct gaaagcgctg ggtcacggcg acggtaagag
    11401 cgccagcagc tactaagtaa gatgctttac gcctgatgcg ctgcgcttat caggcctacg
    11461 agacgtatct acccgtaggc cggataaggc gtagacgcat ccggcaaaag ccgcctcata
    11521 ctcttttcct cgggaggtta ccttgtttct cgcacaagaa attattcgta aaaaacgtga
    11581 tggtcatgcg ctgagcgatg aagaaattcg tttctttatc aacggtattc gcgacaacac
    11641 tatctccgaa gggcagattg ccgccctcgc gatgaccatt ttcttccacg atatgacaat
    11701 gcctgagcgt gtctcgctga ccatggcgat gcgagattca ggaaccgttc tcgactggaa
    11761 aagcctgcat ctgaatggcc cgattgttga taaacactcc accggtggcg tcggcgatgt
    11821 gacttcgctg atgttggggc cgatggtcgc agcctgcggc ggctatattc cgatgatctc
    11881 tggtcgcggc ctcggtcata ctggcggtac gctcgacaaa ctggaatcca tccctggctt
    11941 cgacattttc ccggatgaca accgtttccg cgaaattatt aaagacgtcg gcgtggcgat
    12001 tatcggtcag accagctcac tggctccggc ggataaacgt ttctacgcga cccgtgatat
    12061 taccgcaacc gtggactcca tcccgctgat cactgcctcg atcctggcga agaaactggc
    12121 ggaaggtctg gacgcgctgg tgatggacgt gaaagtgggt agcggcgcgt ttatgccgac
    12181 ctacgaactc tctgaagccc ttgccgaagc gattgttggc gtggctaacg gcgcaggcgt
    12241 gcgcaccacc gcactgctca ccgacatgaa tcaggtgctg gcctccagtg ccggtaacgc
    12301 ggttgaagta cgtgaagcgg tgcagttcct gacgggtgaa tatcgtaacc cgcgtctgtt
    12361 tgatgtcacg atggcgctgt gtgtggagat gcttatctcc ggcaaactgg cgaaagatga
    12421 cgccgaagcg cgcgcgaaat tgcaggcggt gctggacaac ggtaaagcgg cagaagtctt
    12481 tggtcgtatg gtagcggcac aaaaaggccc gaccgacttc gttgagaact acgcgaagta
    12541 tctgccgaca gcgatgctga cgaaagcagt ctatgctgat accgaaggtt ttgtcagtga
    12601 aatggatacc cgcgcgctgg ggatggcagt ggttgcaatg ggcggcggac gccgtcaggc
    12661 atctgacacc atcgattaca gcgtcggctt tactgatatg gcacgtctgg gcgaccaggt
    12721 agacggccag cgtccgctgg cggttatcca cgcgaaagac gaaaacagct ggcaggaagc
    12781 ggcgaaagcg gtgaaagcgg caattaaact tgccgataaa gcaccggaaa gcacaccaac
    12841 tgtctatcgc cgtatcagcg aataacggta tactgatctg atcatttaaa tttgaagcac
    12901 tgagtacgga gaacatatga aacgtgcatt tattatggtg ctggactcat tcggcatcgg
    12961 cgctacagaa gatgcagaac gctttggtga cgtcggggct gacaccctgg gtcatatcgc
    13021 agaagcttgt gccaaaggcg aagctgataa cggtcgtaaa ggcccgctca atctgccaaa
    13081 tctgacccgt ctggggctgg cgaaagcaca cgaaggttct accggtttca ttccggcggg
    13141 aatggacggc aacgctgaag ttatcggcgc gtacgcatgg gcgcacgaaa tgtcatccgg
    13201 taaagatacc ccgtctggtc actgggaaat tgccggtgtc ccggttctgt ttgagtgggg
    13261 atatttctcc gatcacgaaa acagcttccc gcaagagctg ctggataaac tggtcgaacg
    13321 cgctaatctg ccgggttacc tcggtaactg ccactcttcc ggtacggtca ttctggatca
    13381 actgggcgaa gagcacatga aaaccggcaa gccgattttc tatacctccg ctgactccgt
    13441 gttccagatt gcctgccatg aagaaacttt cggtctggat aaactctacg aactgtgcga
    13501 aatcgcccgt gaagagctga ccaacggcgg ctacaatatc ggtcgtgtta tcgctcgtcc
    13561 gtttatcggc gacaaagccg gtaacttcca gcgtaccggt aaccgtcacg acctggctgt
    13621 tgagccgcca gcaccgaccg tgctgcagaa actggttgat gaaaaacacg gccaggtggt
    13681 ttcggtcggt aaaattgcag acatctacgc caactgcggt atcaccaaaa aagtgaaagc
    13741 gactggcctg gacgcgctgt ttgacgccac catcaaagag atgaaagaag cgggtgataa
    13801 caccatcgtc ttcaccaact tcgttgactt cgactcttcc tggggccacc gtcgcgacgt
    13861 cgccggttat gctgcgggtc tggaactgtt cgaccgccgt ctgccggagc tgatgtctct
    13921 gctgcgcgat gacgacatcc tgatcctcac cgctgaccac ggctgtgatc cgacctggac
    13981 cggtactgac cacacgcgtg aacacattcc ggtactggtt tacggcccga aagtaaaacc
    14041 gggctcactg ggtcaccgtg aaaccttcgc ggatatcggc cagactctgg caaaatattt
    14101 tggtacttct gatatggaat atggcaaagc catgttctga tggatttggg cggagcgttg
    14161 actccgcctt tgttatgtca caaaaaggat aaaacaatgg ctaccccaca cattaatgca
    14221 gaaatgggcg atttcgctga cgtagttttg atgccaggcg acccgctgcg tgcgaagtat
    14281 attgctgaaa ctttccttga agatgcccgt gaagtgaaca acgttcgcgg tatgctgggc
    14341 ttcaccggta cttacaaagg ccgcaaaatt tccgtaatgg gtcacggtat gggtatcccg
    14401 tcctgctcca tctacaccaa agaactgatc accgatttcg gcgtgaagaa aattatccgc
    14461 gtgggttcct gtggcgcagt tctgccgcac gtaaaactgc gcgacgtcgt tatcggtatg
    14521 ggtgcctgca ccgattccaa agttaaccgc atccgtttta aagaccatga ctttgccgct
    14581 atcgctgact tcgacatggt gcgtaacgca gtagatgcag ctaaagcact gggcattgat
    14641 gctcgcgttg gtaacctgtt ctccgctgac ctgttctact ctccggacgg cgaaatgttc
    14701 gacgtgatgg aaaaatacgg cattctcggc gtggaaatgg aagcggctgg tatctacggc
    14761 gtggctgcag agtttggcgc gaaagccctg accatctgca ccgtgtctga ccacatccgc
    14821 actcacgagc aaaccactgc cgctgagcgt cagaccacct tcaacgacat gatcaaaatc
    14881 gcactggaat ccgttctgct gggcgataaa gagtaattgt gtttcgctga aaggcgattg
    14941 tcgtgttaag ccggagtggg ggactgctcc ggctttttag tatct
//
LOCUS       DAZKIF010000111        14953 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZKIF010000111 DAZKIF010000000
VERSION     DAZKIF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.112"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..169
                     /locus_tag="ACHOTW_003579"
     CDS             <1..169
                     /locus_tag="ACHOTW_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845618.1"
                     /translation="VTREIQDEIKSKIQDKIDELSKNLFNTMTETIENNFDDIFRQQS
                     ENMSNYYEFVD"
     gene            complement(347..862)
                     /gene="pgpA"
                     /locus_tag="ACHOTW_003580"
     CDS             complement(347..862)
                     /gene="pgpA"
                     /locus_tag="ACHOTW_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9845619.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(840..1817)
                     /gene="thiL"
                     /locus_tag="ACHOTW_003581"
     CDS             complement(840..1817)
                     /gene="thiL"
                     /locus_tag="ACHOTW_003581"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP9845620.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1895..2314)
                     /gene="nusB"
                     /locus_tag="ACHOTW_003582"
     CDS             complement(1895..2314)
                     /gene="nusB"
                     /locus_tag="ACHOTW_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9845621.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2334..2804)
                     /gene="ribE"
                     /locus_tag="ACHOTW_003583"
     CDS             complement(2334..2804)
                     /gene="ribE"
                     /locus_tag="ACHOTW_003583"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP9845622.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2893..3996)
                     /gene="ribD"
                     /locus_tag="ACHOTW_003584"
     CDS             complement(2893..3996)
                     /gene="ribD"
                     /locus_tag="ACHOTW_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP9845623.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(4000..4449)
                     /gene="nrdR"
                     /locus_tag="ACHOTW_003585"
     CDS             complement(4000..4449)
                     /gene="nrdR"
                     /locus_tag="ACHOTW_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9845624.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4600..5139
                     /gene="yajI"
                     /locus_tag="ACHOTW_003586"
     CDS             4600..5139
                     /gene="yajI"
                     /locus_tag="ACHOTW_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9845625.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5438..6322
                     /gene="tsx"
                     /locus_tag="ACHOTW_003587"
     CDS             5438..6322
                     /gene="tsx"
                     /locus_tag="ACHOTW_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP9845626.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6499..6846)
                     /gene="yajD"
                     /locus_tag="ACHOTW_003588"
     CDS             complement(6499..6846)
                     /gene="yajD"
                     /locus_tag="ACHOTW_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP9845627.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6934..7215)
                     /locus_tag="ACHOTW_003589"
     CDS             complement(6934..7215)
                     /locus_tag="ACHOTW_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9845628.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(7208..7450)
                     /locus_tag="ACHOTW_003590"
     CDS             complement(7208..7450)
                     /locus_tag="ACHOTW_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP9845629.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(7601..8572)
                     /gene="secF"
                     /locus_tag="ACHOTW_003591"
     CDS             complement(7601..8572)
                     /gene="secF"
                     /locus_tag="ACHOTW_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP9845630.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8583..10430)
                     /gene="secD"
                     /locus_tag="ACHOTW_003592"
     CDS             complement(8583..10430)
                     /gene="secD"
                     /locus_tag="ACHOTW_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP9845631.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(10458..10790)
                     /gene="yajC"
                     /locus_tag="ACHOTW_003593"
     CDS             complement(10458..10790)
                     /gene="yajC"
                     /locus_tag="ACHOTW_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9845632.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10813..11940)
                     /gene="tgt"
                     /locus_tag="ACHOTW_003594"
     CDS             complement(10813..11940)
                     /gene="tgt"
                     /locus_tag="ACHOTW_003594"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9845633.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11995..13065)
                     /gene="queA"
                     /locus_tag="ACHOTW_003595"
     CDS             complement(11995..13065)
                     /gene="queA"
                     /locus_tag="ACHOTW_003595"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9845634.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            13158..13739
                     /gene="acpH"
                     /locus_tag="ACHOTW_003596"
     CDS             13158..13739
                     /gene="acpH"
                     /locus_tag="ACHOTW_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP9845635.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13760..>14953)
                     /locus_tag="ACHOTW_003597"
     CDS             complement(13760..>14953)
                     /locus_tag="ACHOTW_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845636.1"
                     /translation="ILPILHGCPVESIEVIKNAKRTGQWSYEMTDKNGQSIEVSYIFT
                     DTIDDKSDGKLCLKICNSCNQNDSSTHEYKIDHQQLKNHIRQLEEWALLEKFYDNNAY
                     YSKRTKQLEKKIADAGFITRKVKAENFNVKEFKDDIASMLCEQPPMRTHQISSSGYVS
                     ADKLHKLYKTNLEDAQRDLKTTPVDIDGVSLKSITREKIAEEIAKKTAEIIAKQNAKS
                     IEEKNIEKNKKQTNSEDNFVLKFSPEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDA
                     FSQGLHNVARNGSPRYAGLLRQIASKSAILQDFDQQLFWRTFTGGVLHRHISVKSEPE
                     KTDVWRFSSQFDFPHINENGELSRTVLMFEGVAESNFKDIANHSSECFSYRCYAAPLD
                     ANAKK"
BASE COUNT         3740 a         3957 c         3535 g         3721 t
ORIGIN      
        1 ggttacccgt gaaattcaag atgaaataaa atcaaagatt caagacaaaa tagatgaact
       61 ttctaaaaat ttattcaaca ctatgaccga aaccattgag aataattttg atgatatttt
      121 tcgtcaacag tcagagaata tgagcaatta ttatgagttc gtcgactgac gatatttcag
      181 tgtcattgaa ttatgagtga caaagataca ccttgaaata tcgccgcata gtatattcgg
      241 cgatgtcggg ttttacttta acagagtgcc ccaaaattgg ggcactgttc atttataaat
      301 caaaccgggt ggtctggaat cgcctacacc tcccggtcaa tggcatttaa agaataccca
      361 gcggccagtg atgaccgata aagtacagga tgcctgcgga aatcaccccg gcgacaatat
      421 catcgatcat gatccccatg ccgccatgta cattgcgatc aaaccagcgg atcggccacg
      481 gcttccacat atccagaata cggaaaatca caaacccggc ggcgacccac tgccagtcat
      541 tggtcggcag cgccatcagg gtgatccaca tgccaataaa ctcatcccat acgatgctac
      601 cgtgatcgtg cacccccatg tctttcgccg tttgatgaca aagatagacg ccgatacaga
      661 tccccaacat caccaccagc gagtagagct gccagggcaa aaaggtcatc agataccaga
      721 acggaatcgc cgccagcgat cccatcgtgc caggaacgat tgggcttaat ccacttccga
      781 atccgacagc aagcagatgc cacggattac tcatcttcag gcgacttttc gcgacatctt
      841 tatggcgtgg caaaatggtc atatcctttc cagtctaacg tgacaggttc gccgtcacga
      901 ataaaacaaa gcccttcgat atcggcggtc atttgcccga tacaggtaaa cggtacgccc
      961 agatgaccga gagccacatc cagcgcgcca cggttcagtt ccggcaccgt gaaacacaac
     1021 tcgtaatctt caccgccaga gagcgcccag cgcagcgcct gttccggtac aacatggcga
     1081 gaaagcgcat cagaaaacgg cagcaatgcc aggtcaatac gtgcgccgca gtcgctggct
     1141 ttcacgatat gcccgagatc ggaaatcaga ccgtcagaga gatcgatagc tgaatttgcc
     1201 agatcgcgca gtgcctgccc ctgtaaaata cgcggcgatg gacggagatg acgtttgatc
     1261 aagtagtccg catctttagc atcggcaacc tgcaaacggt tttgcaaaat cgccagcccg
     1321 gcggcgctat cgcccggtgt accggtcaca tagatccagt cacccggttt cgccccagag
     1381 cgcgttaagg ctcgtcccat cggaacaaag ccgtggatac ccaacgtcat tgataatggc
     1441 ccacgcgtgg tatcgccgcc aatgagttgc atatcgtaat aattgagaag atcaaacaaa
     1501 ctgtcgctga aggactcaag ccacgcttcg tctacgtccg gtaaggttaa tgccagcgtc
     1561 agccaggccg gatcggcccc catcgctgcc agatcgctta ggttcaccgc cagtgcttta
     1621 taagccagat cagcaggatc gatatcaggg aggaaatggt tgcccgccac cagcgtatca
     1681 gtgctgatcg ccagggtctg tttctcgggg atattgagaa gagcgcaatc gtcgccgatg
     1741 cccagttcga catcaagacg agaacttctt acacggtcaa aataacgggc aatcagggag
     1801 aactcgccac atgccatacg ttatgcctca gcaggtaaaa aagaaaaggc cggcgatcgc
     1861 ggaacagtct tccgtgaatc taccggcctg gatattactt tttgttaggg cgaatcacag
     1921 gtgctgcttt atcgagtacg ccgttgacga acttatggct gtcttctgcg ccgaacgatt
     1981 tcgccagttc gatcgcttcg ttaatggcca ctttgtatgg cacatcgcta cgtttagaca
     2041 gttcgtacag cgcaatgcgc agtactgctt tttctacctg tcccagttct tccagcaggc
     2101 gggacaggta tggcttcatc agtccgtcga ggtatgcggt attagtcgcc accccggcca
     2161 gcagctcacg gaagtacagg acgtcaacgt cttttacatc ctgttcagcc aggaactggt
     2221 attcaacatc agcgatgtcg ttctgggaca actgccagga gtagagcgcc tggacggcac
     2281 actcacgagc gcggcgacga gcagcaggtt tcacggattt ccccttacta atttcaggcc
     2341 ttgatggctt tcaatacatt aatcatttca agcgcggtca gtgcagcttc tgcacctttg
     2401 ttgccagctt tggtgccagc acgttcgatc gcttgttcaa tgctttcagt ggtcagaacc
     2461 ccaaaagcaa ccggaatttc gctgtcctgg gcaacatgcg ccaggccgtt gcttgcacca
     2521 ccagcgacat attcaaaatg ggcagtgcca ccacgaataa ccgtacccag cgcaatcacc
     2581 gcgtcgtatt taccggtttt agccagtgca cccgccgcca gcggcagctc ataggcacca
     2641 ggcacccaaa caacggtaat gttttcatct tttacctgac cgatacgttt cagtgcatca
     2701 attgcacctt ccagcaggct gtcattgata aagttgttga aacgcgcgat ggtgatggcg
     2761 acgcgagcgt ccggggtagc aacgttagct tcaataatgt tcattttctt ccttcgggtt
     2821 cgagtatggc cccgcagggg ggcggatttt agcataatat ttcgtgcgct gcttcccttt
     2881 cgagccggga gatcatgcac ccactaaatg caggcaaaca tccgggccta catgacgtat
     2941 ctctttgaat ttaaattggg gggcgtcagc taatttctca agccctggca gtgtgcataa
     3001 tccacgggcg tcgctgccta atagtttagg tgcgatatag acaatcagct catcgactaa
     3061 ccccgcctgc agcaatgcgc cagcgagcgt tggccccgct tccacccaga tgctgttaat
     3121 ctgctgttta cccagttgca tcatcagtac aaccagatcc agatgacctt tatgctctgg
     3181 aatcagcaag gtacgcaccg tttccggcca ctcacgagaa tcttcctgcg tacgcgcgaa
     3241 ccaggtttcg ccgggctgct gcacaatgtg atgttccggc gtcacgcgat tttggctatc
     3301 aatcacaata cgtaccggct ggcggagatg atgttgcggg tagagcgcct gagtttgtac
     3361 atccagttca gaccaacgca ccgttaaggc aggatcatcc gccagcaccg tagcgctgct
     3421 ggttaaaatg gcatgacttt gtgcgcgcag tcgttgtacg tcgcgccgcg cctgaggcga
     3481 agtgatccac tggctttcgc cgctcgccat cgccgtgcgg ccatcaagcg atgcgccaag
     3541 ttttaactga atataaggaa agccggtgcg catccgcttg agaaagcctt tattcaattg
     3601 ctcggcttca ctcatcatca ggccgtggct gacgtcaatg ccagcctgtt gcagacggta
     3661 aagtccacgc ccagcgacct gcgggttcgg gtcctgcatc gcagcgacca cgcgcgctac
     3721 gccagcggcg atgagtgcgt cacagcacgg tggcgtacga ccatgatggc tacagggttc
     3781 gagtgtgaca taggcggtcg cacctttggc tttttcaccc gccatacgca acgcgtgtac
     3841 ttcggcatgt ggttcacccg ctcgttggtg gtaaccttca ccgacaattt cgccatcttt
     3901 gacaatgacg cacccgacat tcgggttggg gtgggtggta aaacgtcctc gttgcgccag
     3961 ctttagcgcc cgcgccatgt aatactcgtc ctgcacggct tagtcctcca ggcgcgcgat
     4021 ctcttcgcca aattctttga tatcttcgaa actgcggtag acagaggcaa aacggatata
     4081 ggcgacttta tcgagctttt tcaattgctc catcaccaga ttgccaatca tcttgctcgg
     4141 cacttcgcgc tcaccggtgg cgcgcagctg cgatttaata tgattgattg ccatttcgac
     4201 gtcatcggaa ctcaccggac gtttttccag cgcccgcagc attccgctac gtaatttttc
     4261 ttcattaaac ggttcacgca cgtcgttgct ttttacaaca cgcggcataa ccagctccgc
     4321 cacttcaaag gtggtgaaac gttcattaca caccagacac tgccgacggc ggcgtacgga
     4381 tgaaccctcg cccacgagac gagagtcaat taccttagtg tccacggcga aacagaatgg
     4441 gcaatgcata cggcgtcctg accaggtggt taacggaaat ctattttacc ctgaactgac
     4501 gatacaacaa aggcgcaacg cttttgagat aaaggatcga tgctttcgct gcttttaccc
     4561 tctaccatta ggctaattgc gacgagaaag ggacgaccta tgaatattaa cgtttttcga
     4621 ctgatcctgc tgggaagcct attcagcctt agcgcctgtg tgcagcaaag tgaagtgcga
     4681 cagatgaaac acagcgtcag cacgctgaac caggagatga cgcaactcaa tcaagaaaca
     4741 gtcaaaatca ctcagcaaaa caggctgaat gcaaaatcca gcagtggggt ttaccttctg
     4801 cctggagcga aaacaccggc aagactggaa agccagatcg gtactttacg tatgtcgctg
     4861 gtgaatatta cgcctgatac agatggcacc accctgacac tacgtattca gggtgagtcg
     4921 aatgatccct tgcccgcatt cagcggaact gttgaatatg ggcaaattca gggaacaata
     4981 gacaactttc aggaaatcaa tgtacagaat caattgatta atgcccctgc cagcgtcctc
     5041 gcccccagcg atgttgatat tccgttacag ttaaagggga tttctgtgga tcagttaggc
     5101 tttgtacgta tccacgacat tcaacctgtt atgcagtaaa cttatagcgg ggcgattttg
     5161 tgcgtcccgc aacatccttc cccatcattt tgttactccg cttacataac ccagattgat
     5221 agtaaaagtt tgcaacaagg gcgaaagtca gtacaatccc cgcccgaatg tgtgtaaacg
     5281 tgaacgcaat cgattacgta aatgatagaa ctgtgaaacg aaacatattt ttgtgagcaa
     5341 tgatttttat aataggctcc tctgtatacg aaatatttag aaacgcaatt tgcgcctttt
     5401 tcactcccgc aagggatttt caaacagtgg catacatatg aaaaaaacat tactggcagc
     5461 cggtgcggta ctggcgctct cttcgtcttt tactgtcaac gcagctgaaa acgacaaacc
     5521 gcagtatctt tccgactggt ggcaccagag cgttaacgtt gtcggaagct atcacacccg
     5581 tttcggaccg cagatccgca acgataccta ccttgagtac gaagcattcg ctaaaaaaga
     5641 ctggttcgac ttctatggtt atgcggatgc gccggtattc ttcggcggta actccgatgc
     5701 aaaaggtatc tggaaccacg gttctcctct gtttatggaa atcgaaccac gtttctccat
     5761 cgacaagctg accaatactg accttagctt cggtccgttc aaagagtggt acttcgcgaa
     5821 caactacatt tacgacatgg ggcgtaataa agatggtcgc cagagcacct ggtacatggg
     5881 tctgggtacc gatatcgaca ctggcctgcc gatgagcctg tccatgaacg tctatgcgaa
     5941 ataccagtgg cagaactatg gcgcagcgaa cgaaaacgag tgggacggtt accgtttcaa
     6001 aattaaatac tttgtgccga ttaccgatct gtggggcggt cagctgagct acatcggctt
     6061 caccaacttc gactggggtt ccgatttagg ggatgacagc ggtaacgcaa tcaacggtat
     6121 taagacccgt actaataact ctatcgcttc cagccatatt ctggctctga actacgatca
     6181 ctggcactac tctgtcgtag ctcgttactg gcacgacggt ggtcagtgga acgacgatgc
     6241 agaactgaac ttcggcaacg gcaacttcaa cgttcgctct accggctggg gtggttacct
     6301 ggtagtaggt tacaacttct gattatgaaa atgccgggat ttattcccgg catttcagat
     6361 tgttaacaac gtacgctttg ttcatgccgg atgcggcgta aacgctttgt ccggcccaca
     6421 aaagcacgta aattcaatat attgctgaaa taacgtaggc ctgataagcg tagcgcatca
     6481 ggcaatttta cgttttaatc acttcttctt gttcatcatc gctttcaggt cagcgaatgg
     6541 gttgtacttc gcttcaccga catctttctg cgcatcttcc cctgcgataa cggtcgtacc
     6601 atactgatcc gcttcggtat atttcgaatg ctcatgatcg tggcaataga gacaaagcaa
     6661 ttcccagtta ctgccatctt ccgggttatt ggtatggtcg tgatcaatgt ggtgaacggt
     6721 aagttcacgc aggttggaat aaacaaactc gcgggaacag cgaccgcaga cccacggata
     6781 gatttttaat gctttttcgc gatagccgct ttctaaccgc gcatagtttt ttgggatgat
     6841 agccatgaaa agttacctgg atgaaatgta gggttacgcg cagtttccca agatgcgggg
     6901 attttcgcaa tcctgagtca agaaattctc acattaccag tgacggcgag gttccatcaa
     6961 ctgatgaaga atgcgcgcaa tgagaatgtc atcgactctt ataacgtaat aaacagtgtg
     7021 aaacctaaat gcgatcttct ttacctctgg aatcgcatca tagtcttgcc ctatctcggg
     7081 catttgagag agtgtatcca gaaaagtgcc gagagagtcg gtatagcgat ccgcctgctc
     7141 atcaccaaac tttcccagcg tgtagtcata gatccctctg aaatcttcaa cagcttttcc
     7201 gctaagctta tacacgatgt ttttgcttcc tttctgctgc gatatcacga agcgtcatcg
     7261 aactttcacc gctctcaaga ccttcaataa cagcatttct gacagcttca tccctggatt
     7321 cttgttgttc caatagacga agtgccgaac gcattacttc actggccgag ccataacgac
     7381 cagactggac catccgggta ataaaactat ctaattgttc gccaacgctt actgaggtaa
     7441 ttttgcgcat aagcgatctc cacgtattaa ctattaatac aaagtatatg tcagaacgct
     7501 ttctacaacc aaaccgtatt cagttgcctg tgaaacaggc aaaaaaaacc gcagcgcaaa
     7561 tacgccacgg tttttttaac agaacggatt taaaacacca ttacggcaga attgacggct
     7621 gatccgcccc ttctttttcc actttctgct gcaacatgtg ttcgcgcttc atacccagtt
     7681 tcagagccaa cgcagatgcc acatagatgg aagatgcagt accgatggaa acaccgataa
     7741 gcatggtcag cgagaagcct tccagtaccg gaccaccgaa gagatacagc atcaggataa
     7801 ccatcaaggt agtaccggat gtgatcaagg tacggtgcag cgtctgggtc aaggacacgt
     7861 taaagatttc gtaaggcgta ccgcgacgga tcttgcggaa gttttcacga attcggtccg
     7921 ataccacgat actgtcgtta agcgagtaac cgataaccga catcaacgat gccacaatgg
     7981 tcaggtcaat ctcgatatgg aataacgaca aaatacccag cgtaatgata acgtcgtgcg
     8041 ccagcgcaat aaccacccct gccgccagtc gccactcaaa gcggaaacct acgtacacga
     8101 ggatagacag cagcgctgcc atcaacgcca tcgcaccggt ttgcgcaagg tctgccccca
     8161 cgctcggacc gacgaactca atacgcttca ctgctgcatt ctgattggtg gattcgttaa
     8221 tcaccttcag aacctggctg cccagcacct gaccgccggt ttcgccttca gcaggcggca
     8281 tacggaccat gatgtcgtgg ctgctaccaa agttttgcag catcggctct tcaaaaccgg
     8341 ctttttgcaa tgcatcacgc attacgtcaa tttcagccgg tttttcgagc gtaatttcga
     8401 taaccgtacc accggtgaaa tccagccccc agttaaagcc gcgcacgccc ataataacga
     8461 tagcagcgat taacagcaga ccagagatgc caaaagccca gtagtcccag cgcataaagt
     8521 catagacttt acggccgtgg tttagttgtt caacagtata ttcctgtgcc acatcgcact
     8581 cctcagattg acagcttctt gacgcgcttg ccgccatata gcaggtttac gatggcacgc
     8641 gtaccgacaa tcgcggtaaa catcgacgtc gccacaccga taccggtagt aatcgcgaac
     8701 cctttaattg ccccggtacc cactgcgtac aggatgatga ctttaatcag cgtggtgatg
     8761 ttcgcatcga agatagaact gaatgcgcca cgataacctt catcaattgc ctgttgaaca
     8821 gtacgtccgt tgctcaactc ttctttaata cgttcgttga tcagtacgtt cgcatcgacc
     8881 gctaccgcaa gggttaagac gatacccgcg atacctggca tactcagtgt tgcgcctggc
     8941 aacagcgaca taataccgac gatcaagatc aagttggcaa tcagagcact ggtcgcaatc
     9001 agaccaaact tcttatagaa gatgatcatg aacagaatag aaaccagcag accggcaagg
     9061 caagcctcca gcccctgctc aatgttctgc atacccaggg tcgggccaat tgtacgttct
     9121 tcaacaatct gaatcggcgc gatcaacgca ccggcacgca gcagcagtga cagctgacgg
     9181 gcttcgttcg ggttgttgat gccggtgata cggaagctgt tacccagacg agactggatg
     9241 ttggcgatgt taatcacctc ttcctgtttc accagaaccg cacgaccatt tgcatctttc
     9301 ttaccgctgt ctttgtactc cacaaacagg gttgccatcg gtttgccgat gttgtccttg
     9361 gtgaagttag acataatgtt gccaccagcg ctatcgagcg agatgttaac ctgcggctgg
     9421 ttgtattcgt cctggctgga agtggagtcg gtgatatggt caccggtaag aattacgcgt
     9481 ttgtacagca caaccggctg accttcgcgg gtctgtttca cttcagagtc gcccggtacg
     9541 cgaccggatg ccgcagcggc ctgatcaacg ttggtgttta ccagacggaa ttccagcgtt
     9601 gccgtcgcac ccagaatctc tttcgcacgc gcagtgtcct gaatacctgg cagttcaaca
     9661 acgatacggt cagcaccctg acgctgaacc accggctccg ccacgccaag ttggtttaca
     9721 cggttacgca ggatattgat gttctgctgc accgcatatt cacgcgcttc actcagacga
     9781 gcatcgctca ttaccgcacg cagctggttg ctgccctgac tgctaatcac caggtccgga
     9841 tgacgcttgc tcagatacgc aatggcttca tcacgagctt tagcatcgcg gaaagtgatg
     9901 ctcaggccgt agttattttc tttacgaacg gtggtatacg ggatgccttt ttcgcgcagg
     9961 tcgctgcgca ggctatcgat attttgttcc tggagtttgc caagcgcggt gtccatatcc
    10021 acttccatca ggaagtgaac gccgccacgc aggtcaaggc cgagcttcat cggctcagcg
    10081 tgaatagctg ccagccagcg cggcgttgcc ggggcaaggt taagcgccac gacgtatttg
    10141 tcacccataa cgcccattaa tgcttcacga gcgcgcagct gggtgtcagt ggagtcgaag
    10201 cgcgcaagaa tagcgccctc ttccagtgcc acagacttag cagttatttt ttcttcttgt
    10261 aacgttttct ggacctggat cagcgtttgc tcactggcgg cgactccgcg cgcaccagtg
    10321 atctgaacag ccggatcctc accaaacagg ttgggaagcg catacagcag accgatgaca
    10381 atcaccacga tcagcatgac gtacttccac aaaggataac ggtttaacac ggcaattccc
    10441 ttagggaaaa attttaatta cagcgccttc atggtgcctt tcggcaggac ggcagctacg
    10501 aagtcacgtt taataactac ttcagtggtg tcattcagcg cgatagcaat gtagccgttt
    10561 tccgctactt tggttacgcg accaaccagg ccaccgttcg tcagaacttc atcacctttg
    10621 gcaatggagt ccatcagctt tttgtgttct ttggtgcgct tctgctgtgg acgcaggatc
    10681 atgaaataga aaatcagacc gaataccacc agcatcaaaa tcaaagacat cgggctacct
    10741 tgcgccggtg cacccgttgc cgctaccgca tcagaaataa aaaagctcat taaatttccc
    10801 tcattattaa tattaatcaa cgttcaaagg tggtacttct cgcccctgac gctggtaaaa
    10861 atcagttacg aagctctcta atttaccctc ttcaatagcc ttgcgtaaac ccgccatcaa
    10921 acgctggtag taacgaaggt tatgaatggt attgagtcgc gcgcctaata tttcgttgca
    10981 acggtcaaga tgatgcaagt aagcgcgtga ataattgcga caggtgtagc aatcacactc
    11041 aggatcgagt gggccagtat cgctcttata cttcgcattg cggattttca ccacgccatc
    11101 ggtcacgaac aaatgaccat ttcgggcgtt gcgggttggc attacgcagt caaacatatc
    11161 gataccacga cgtacgcctt caaccaggtc ttctggttta ccaacgccca tcaggtaacg
    11221 cggtttgtct gccggaattt gcgggcatac atgctccaga atgcggtgca tatctgcttt
    11281 cggctcaccc acagccagac cgccgacagc gtagccatca aaaccgatat ctaccagacc
    11341 tttaacagaa atatcacgta aatcttcgta aacgctgccc tgaatgatac caaacagcgc
    11401 atttttgttt ccgagactgt caaaacgctc acggctacgc ttcgcccaac gcagagacat
    11461 ctccatggag cgttttgcgt aatcccagtc agcaggatac ggcgtacact catcaaagat
    11521 catgacgata tccgaaccaa gatcgtactg aatctccatc gacttttccg ggtcgaggaa
    11581 aatcgggtcg ccgttgatcg ggttacggaa gtgcacgccc tgttcggtga ttttacgaat
    11641 atcgccaagg ctgaagactt ggaagccgcc ggagtcggtg aggatcggtc ccttccactg
    11701 cataaaatcg tgcagatcgc cgtgcagttt catgatttcc tggcccgggc gcagccacag
    11761 gtggaaggtg ttgccgagga taatttgcgc gccagtggct tcaacttctt ccggcgtcat
    11821 cccttttacg gtgccgtagg tgccaacagg cataaaacaa ggcgtttcca ctacgccacg
    11881 atcaaagacc aggcggccac ggcgtgcgcg accgtcggtg gtgtccagtt caaatttcat
    11941 tttttctcca ctacgtcaga aaaacagtcc aacgtttaaa ccagcgccgc ggaattactc
    12001 cccgacgcgc tcattaattg cctgcggatt gtacgtgata aacatcgcat caccgtaact
    12061 aaaaaagcga tatttctctt ctaccgccgc tttataggca ttcattgtgt gttgataacc
    12121 ggcaaaggcc gaaaccagca taatcagcgt cgattccgga aggtggaagt ttgtcaccag
    12181 cgcatcgacc actttgtact ggaagcccgg atagataaag atttgggtat cgtcaaagaa
    12241 cggttcgatg agatcgtttt tcgccgcctg agccgcgctt tccagcgaac gtactgaggt
    12301 ggtgccaacc gcaatcaccc ggttaccgcg cgctttcgcc gccagtaccg cgtctaccac
    12361 atcctgcggt acttcagcgt attccgagtg catgatgtga tcttcaatgg tgtcgacgcg
    12421 taccggctgg aaggtgcccg caccaacgtg cagggtgacg aacgccatct ccacgccttt
    12481 ggcgcgtaat ttttccagca aaggttcgtc aaaatgcaga ccagcggtcg gggctgcaac
    12541 cgcgcccggt ttttcgctat aaacggtttg ataaagttcg cggtcagcgt cttcgtccgg
    12601 acggtcgata tacggcggca gcggcatatg gccgatgctg ttgagaatat ccagcaccga
    12661 gcgttcatca ttaaattcga cttcaaacag tgcgccgtgg cgcgcggtca ttgttgcgtt
    12721 aatactttcg tcatcgccca gcagcagttc tgcgccaggt tttggcgctt tcgaggcgcg
    12781 aatatgcgca agaatgcgtt tgtcgtcgag catccgttca accagcactt caatcttgcc
    12841 gccgctggct ttacgcccaa acaggcgcgc cgggatcacc cgggtattat taaaaaccag
    12901 aagatcgccg gggttgagct tatcgagtaa atcggtgaaa gtaccgtgcg tcagcgcgcc
    12961 cgtcggcccg tccagcgaca gtaaacgaca gctactgcgt tcaggcatcg gatagtgggc
    13021 aatcagggat tcgggcaatt caaaggagaa atcggtaacg cgcatgacac tgactcagac
    13081 taaaataaga ggcgggtagt ctagtgccgg ggcgctctcc ctgcaacctt tacccccctt
    13141 tggatagaat ataatcgatg aattttttag ctcacctgca tttagcccat ctcgcggaaa
    13201 gctcgctttc cggcaatttg ctggctgatt tcgtacgcgg aaatcctgaa gaaagttttc
    13261 cgcccgacgt cgtggctggc attcatatgc atcgacgtat cgacgtattg actgacaatc
    13321 tgccggaagt ccgcgaagca cgggagtggt ttcgtagtga aactcgccgc gttgcgccta
    13381 ttacgctgga tgtcatgtgg gatcactttc tttcccgtca ctggtcgcag ctgtcgccgg
    13441 actttccgct acaggaattt gtttgttatg cccgcgagca agtgatgaca attttgccgg
    13501 actcaccgcc acgctttatc aacctgaaca actacttatg gtcagagcag tggctggtgc
    13561 gctatcgcga tatggatttc atccagaacg tgttaaacgg catggcaagc cgtcgcccac
    13621 gtctggatgc cctgcgtgac tcctggtacg atttagacgc ccattatgcc gccctcgaaa
    13681 cccgcttctg gcagttttat ccacggatga tggcgcaggc gtcacgcaag gcgctctgaa
    13741 acttcaaact ccggcgtcac tattttttag cattcgcatc cagcggggcg gcataacaac
    13801 ggtaagaaaa acattcgctg gaatgattgg cgatatcttt aaagttgctt tcagcaaccc
    13861 cctcgaacat gagtaccgtt ctggacaact ctccgttttc atttatatgt ggaaaatcaa
    13921 attggctact gaatcgccat acatctgtct tttctggctc gctcttcaca gagatatgtc
    13981 gatggaggac ccctcctgta aacgtgcgcc agaacaattg ttgatcaaaa tcttgtaata
    14041 tcgcagactt ggatgctatt tgacgtagta aacctgcata ccgtggactg ccattacgag
    14101 caacattatg caaaccctga ctaaaagcat caaacaaaca atagatgtga tcactagtga
    14161 aaaaaggtct taattttttt tgttcttcat cgctgtaatc caggagcatc tcattgattt
    14221 cttccggtga gaattttaag acaaaattat cctcagaatt tgtttgtttc ttgttttttt
    14281 caatattctt ctcctcaatg ctcttcgcat tttgtttcgc aattatttcc gcagtttttt
    14341 tcgcaatttc ttccgcaatt ttttcccgag ttattgattt tagactaaca ccatcgatgt
    14401 caaccggtgt tgttttcaga tctctctgcg cgtcttccaa atttgtctta tagagtttgt
    14461 gcagtttatc ggcagatacg tatccggatg aagaaatctg gtgtgtacgc atggggggct
    14521 gttcacaaag catcgaggct atatcgtctt taaactcttt tacattaaag ttctcggctt
    14581 taaccttcct tgttataaac cccgcatcgg caattttctt ctctaattgt tttgtacgtt
    14641 tagaataata agcgttgtta tcataaaact tttcaagaag ggcccactcc tcaagttgcc
    14701 ttatgtgatt tttcaactgt tgatgatcta ttttatattc atgtgtacta gaatcatttt
    14761 ggttgcagga gttacagatt tttaagcata attttccatc agatttatca tctatggtat
    14821 cagtgaaaat ataggacact tcaatactct gcccgttctt atcagtcatc tcatatgacc
    14881 attgtcccgt tcgtttcgca ttcttaatga cttcgatcga ctctacagga caaccatgaa
    14941 gaatgggcag aat
//
LOCUS       DAZKIF010000112        14933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZKIF010000112 DAZKIF010000000
VERSION     DAZKIF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.113"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            78..347
                     /locus_tag="ACHOTW_003598"
     CDS             78..347
                     /locus_tag="ACHOTW_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9845637.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            316..1434
                     /locus_tag="ACHOTW_003599"
     CDS             316..1434
                     /locus_tag="ACHOTW_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9845638.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1601..2395
                     /gene="potC"
                     /locus_tag="ACHOTW_003600"
     CDS             1601..2395
                     /gene="potC"
                     /locus_tag="ACHOTW_003600"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP9845639.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2392..3438
                     /gene="potD"
                     /locus_tag="ACHOTW_003601"
     CDS             2392..3438
                     /gene="potD"
                     /locus_tag="ACHOTW_003601"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP9845640.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHOTW_003602"
     CDS             complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHOTW_003602"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP9845641.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHOTW_003603"
     CDS             complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHOTW_003603"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP9845642.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHOTW_003604"
     CDS             complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHOTW_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP9845643.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHOTW_003605"
     CDS             complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHOTW_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP9845644.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHOTW_003606"
     CDS             complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHOTW_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP9845645.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHOTW_003607"
     CDS             8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHOTW_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9845646.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            9971..13417
                     /gene="mfd"
                     /locus_tag="ACHOTW_003608"
     CDS             9971..13417
                     /gene="mfd"
                     /locus_tag="ACHOTW_003608"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9845647.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHOTW_003609"
     CDS             13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHOTW_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP9845648.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHOTW_003610"
                     /gene_synonym="ycfR"
     CDS             complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHOTW_003610"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP9845649.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         3730 a         3877 c         3812 g         3514 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccgtcgc cagttgtcgt tgatattctg caactggcgc
       61 gttccggagt gatagccatg agcgaacagt acctgataac gctcgacgag tggaaaccaa
      121 aacggttcag tctcccaata acaaacacta ccctggtgaa atacggaaaa ctaggataca
      181 tcgttccaag accacaaaaa attcgtgggc gttggctgat agatcgccga gcagtatttg
      241 ttgggcctgg tgaaacggga attgcgccgg aaattcatac tggcgatgat gatgcactga
      301 aggagatttt aactcatgtc accgaggcca cgaaaaaaca gcactgacgt agccggtctt
      361 tacgaaaagt ttgatcgcag aactggcaga gtttactacc agtataaaaa tcctgtgact
      421 ggaaaatttc acggactcgg aacagacaaa ggtaaggcag aaaaaatcgc ttccacagcc
      481 aatcagcgaa tagctgcagc agaagctgaa tatttcatgc gcaaaattga tgaaagtccg
      541 tcagcaacaa aacgtcgggg tatcagatta aaggcatggg ttgatcgata tctgaaaata
      601 caggacacgc gactgaaaaa tggagatatt gcagctacaa ctcacaaaga aaaaactcga
      661 atggctgcat acctggtttc ccgtctggga aaccacccat tgaaagaact ggaagtaaga
      721 gactttgcat taatactgga tgagtggctg gataaagaca tggtcagtac agcgagagta
      781 aatcgtggat tatgggttga tatttataaa gaagcacagc atgcagggga agttcctcct
      841 ggatggaatc ctccggaggc tacccgtaaa ccgatcccta aagtaaccag agccaggctc
      901 accatggaag actggcaaaa aatttacaat gcaacgcctg aaaaacactt tatccgtaac
      961 gcaatgcttc ttgcgattgt tactggtcag cgccgtgatg acatttgcca catgcgtttt
     1021 tcagatgtgt ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg
     1081 ttaccgctta cactacgctg tgatgccatt gggataacgt taaaagaagt tattgatggg
     1141 tgccgagaca gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccgaag
     1201 ccgatgagta aagacaacct gagcgactac tttgccaaag cacgggatct ggctggggta
     1261 attccaccag caggaaaaac tccgccaaca tttcatgaac aacgctctct atcagaacgg
     1321 ctgtaccgtg cacagggtat cgatacaaaa acattactag gacataaagt ccaggcaacc
     1381 accgatcgct ataacgatac tcgaggtcag gaatgggtta agttggttat ttgacaaaca
     1441 ccatatggaa tatccgtcac catctcagga aaaaagtgtt gtaaaatgcg tggcctagtt
     1501 ttggggaaac gttttggaga gattttggag aaaggaaaaa attgaataaa ttcaaaatcc
     1561 tgaaagtgtc ccgccttatg aataaaaagg tggaactcga atgatcggtc gactgcttcg
     1621 cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt
     1681 gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta
     1741 tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc
     1801 ggtgttttcg gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg
     1861 ttatcgcttt cgtggtaagc cgttcgttag cggaatgctg tttgtggtga tgatgtcacc
     1921 agatatcgtg atggcgattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg
     1981 cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt
     2041 gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc
     2101 cagcgaattt accattctgc gaaaaatcat tctgccactg gcaatgccag cggtggcggc
     2161 aggctgggtg ttaagcttta ccctgtcgat ggacgatgtg gtggtttctt cgttcgtcac
     2221 cgggccgagt tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc
     2281 gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc
     2341 cagccagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa
     2401 tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc
     2461 gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt
     2521 gaacagttca ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa
     2581 accatgtatg ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca
     2641 acctattacg tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag
     2701 ttaacgaact tcagcaatct cgatccagac atgctcaata agccgtttga cccgaataac
     2761 gattactcca ttccgtatat ctggggtgcg acggcgattg gcgttaacgg tgatgcggtg
     2821 gatccgaaat ctgtcaccag ctgggccgat ttgtggaagc ctgagtacaa aggcagcctg
     2881 ctgctgaccg acgatgcccg tgaagtgttc cagatggcgc tacgtaagct gggctactcc
     2941 ggtaacacca ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg
     3001 ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt
     3061 aacctcggca tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt
     3121 gacgtggtgt ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca
     3181 aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg
     3241 gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg
     3301 ttaagtccag aagtggcgaa cgataagacg ctctccccgg atgctgaaac cattaaaaat
     3361 ggcgaatggc agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag
     3421 ctgaaagcag gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttagtta
     3481 gcaaggatgt ggctaatgag tcaggacagt aaagttttat cccgctcttt cgtcggtatt
     3541 gtggtccgct tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat
     3601 cccttcagca acttttcaac aaattctggc accacctggc ttgccgggcc gtaatatttc
     3661 tcggcaaatt cattaccgac ctgacttggt tcaagattca gctccacggt gtgcgcgcca
     3721 tgcagtttcg cttcgtgaac aaacccagcc gccggataaa catgcccgga agtaccaatg
     3781 gcaatgaaaa tatcggccat cgacaacgcc atataaattt catccatgcc gagtggcatt
     3841 tcgccaaacc atactacgtg tgggcgcaag ggggccggga actggcagca atggcattta
     3901 tcttctgggg taacgtctcc ggtccagtcg agaacctgac cactttgtga acaacgaact
     3961 ttcagcagtt ccccatgcat atgaatcaca ttggtattac ctgcgcgttc atgcaggttg
     4021 tctatattct gcgtcaccag caaaaagcga tcgccgagag catcttgcag tttagccagc
     4081 gcaagatgcg cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta
     4141 taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca
     4201 acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca
     4261 gaaattcctg cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
     4321 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
     4381 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt
     4441 agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg
     4501 cgcggaacac gagctacagg taagagatga cgaggcagcc tgtccgccag ttgcgttgtg
     4561 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
     4621 aggatatttc ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
     4681 tgctcatcgc cttgatcata aagcgcaata atttcgggag ccggcaacgg ttgatgataa
     4741 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc
     4801 tgaccacagc cgcagcggcg taacgggaaa tccagcccca tcatggttaa cgcatcaacc
     4861 ggcagacgca tatggccaaa ctcgccggta atgtagcttt tcccggtaat cggtttgccg
     4921 ttgaaaatca gcccgccgcc aacgccggtg ccgagaatca accccatcac caacggatat
     4981 tgcgtaaatt cgtcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg
     5041 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagca
     5101 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg
     5161 ccgacagagc ctttacagcc aaaacgttga tcagcttcgg ctaccagctc gcacactgca
     5221 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
     5281 cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac
     5341 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
     5401 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
     5461 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata
     5521 gatatcgctg gagaggaact gatgaccgat cagcttttca atccgctcaa taatcggggt
     5581 aagttgcagt gaaacaacta cgccgataat cacaccacac aggctaccga atagccctgc
     5641 cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct
     5701 taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt
     5761 gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
     5821 gatatcgcga tacatatagc cgtaagtacc aatccagctt ttaatataaa catagctgtt
     5881 ggtcacttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
     5941 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat
     6001 catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa cgtgcaaacg
     6061 cacacgtttt ggctgcatca gtttatgctc aggattcgag ttggggatca taatcgacac
     6121 ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg
     6181 ttcgcccgct ttaaaattgc gccaggcatc cccctgaaca aacgagggta atgcgctcag
     6241 acgctgttcc tgttgcgggt taacgccctt cacctggatt gcgcgcagat tcgctccact
     6301 ttccaccagc ccggtgaaat tgatatacgg cgcagcggcg gcaatacctg gcactttttg
     6361 cacgttatcc agtgcttcct gccagttagt ccacggttga tccaccgcct ctatttcgcc
     6421 atgcggcacc accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct
     6481 taagccgacg atcaataccg ccacgccaag ggcaatgcca atggtagaaa tgacggagat
     6541 cagcgacacc atgccgccgc gtcgccgtcc gcggctaaaa cgcaggccaa tcaataacga
     6601 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgaccatca
     6661 cgcatctcca gttggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
     6721 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtctgcgtta
     6781 cgcgcatcga ggttaccggt aggttcatcc gccagtacca gccacgggtt attgaccagc
     6841 gcacgggcaa tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
     6901 gcacgatgct ccagccccac cgcttttaac atctcaagtg cacggctgtt gatttcagcg
     6961 ggctttttct tgccaatcag cagcggcata gccacgtttt ccagggcagt aaaatccggc
     7021 agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc
     7081 gccgaagaca gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggtgta
     7141 tccagcccgc ccagcaggtg cagcaaggta cttttaccgg aaccagagct accgacgatc
     7201 gccatcattt caccttcgcc cacgctgaaa ctgacattgt gcagtacatc ggtttgcaca
     7261 ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg
     7321 taaagcctca gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag
     7381 cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg
     7441 cagcgccgcg ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc
     7501 aagcagtgcg ccaagcgccg ctccgaggat cgcaccgata atcccggcgc tggccccttg
     7561 caccataaag accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgctac
     7621 ttcgccctgc ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac
     7681 ggcgacaatc aggctcagca gtaagcccat catatttttt tccatgcgta cggcctggaa
     7741 cagctcgcct ttacgatcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg
     7801 acttaaagag tcaactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc
     7861 tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc
     7921 gacttcactg ttagcggcaa aagtaccaat cacattgaac aggcgctgac ttggaatacg
     7981 ccccatcggc gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt
     8041 aacgcctagc tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag
     8101 gtcagtttgt ttcacattga ccagatacgg cgttagtgga tctttttgcg ccggatcgat
     8161 acccagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt
     8221 aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg
     8281 gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt
     8341 ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc
     8401 gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccgctgcacg
     8461 cccacgcatg taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg
     8521 gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg
     8581 taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccatg
     8641 ttttatcatc aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata
     8701 cccattacac ctcgggtcat aacccgcagg atcaaccgca tgtaaattaa aaaagagcgt
     8761 gttgtcctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt
     8821 ctggtggtga tttatcactc ggtcattacc ttttatccgc atatgaccac ttttcagcat
     8881 ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct tgcacccttt
     8941 cgtatgccgg tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta
     9001 ccgtggggaa attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg
     9061 ggcgtggtgc agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat
     9121 ttaagcaatg cctccaatgc cgcgtatgcc gattccaccg gtgagttcct gcacgggatg
     9181 atcaccgcca gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt
     9241 aaaattttta gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg
     9301 gttaatttcg ttcccacgcc gtggtgggga atgaacagtg tgatccgcaa tttgccttat
     9361 tacagccttg gtgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg
     9421 cgccgccatc tgctgatggc ttctttgctg gctgctctgg cggtcggtgc ctggttgttt
     9481 actatctcgc tgctgttgtc gctggtgtcg attgtggtga tcatgaagtt gttttatcag
     9541 tatgaacaac gttttggcat gcgttccacc agtctgctga atgtgattgg ctccaacact
     9601 attgctatct acactaccca tagaattctg gtagagatat tcagcttaac tctgcttgcg
     9661 caaatgaacg cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttacccc
     9721 tttgttagtt tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcga
     9781 gcattcagcg atctgttgtt ctccccgcct tcactgcccg ttgccgtcag ttactcccgc
     9841 taagccatcg ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg
     9901 aacagcattg cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct
     9961 gacaaccgtt atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg
    10021 tctgctgggc gagttaaccg gcgctgcctg cgcgacgctg gtagcggaaa ttgccgaacg
    10081 tcacgccgga ccggtggtgc ttattgcgcc agatatgcaa aatgccctgc gtttgcatga
    10141 tgaaattagc cagtttaccg accagatggt gatgaatctg gcggactggg aaaccctccc
    10201 ttacgacagt ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca
    10261 gctaccgaca atgcagcgtg gcgtactgat tgttccggtg aatacactta tgcagcgcgt
    10321 ttgcccgcac agttttctcc acggtcatgc gctggtgatg gaaaaaggtc agcgcctgtc
    10381 acgagatgca ttacgaaccc aactggatag cgccggttat cgccatgttg accaggtgat
    10441 ggagcacggc gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga
    10501 gttgccttat cgtcttgatt tctttgatga tgaaatcgac agtctgcggg tgtttgacgt
    10561 cgacagtcag cgcacgctgg aggaagtaga agcgatcaac ctgctgcctg cgcacgaatt
    10621 tccgaccgat aaagcggcga ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt
    10681 gaagcgcgat ccggaacata tttaccagca agtgagtaaa ggcacattac ctgccgggat
    10741 cgagtactgg cagccattgt tcttcagcga accactgccg ccgctgttca gttatttccc
    10801 tgccaatacc ttgctggtga atactggcga tctggaaaac agtgccgaac gtttccaggc
    10861 tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccac tgttgccacc
    10921 acaatcgctc tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgagt
    10981 gcagctaaaa actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa
    11041 actgccagac ctggccgttc aggcacaaca aaaagcgccg ctggatgcgc tgcgtaagtt
    11101 cctcgagtct ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggcc gccgtgaagc
    11161 gctgggtgaa ctgctcgcgc gaattaaaat tgctccgcaa cgcattatgc gtcttgatga
    11221 agccagcgac cgtgggcgtt atctgatgat tggcgctgca gagcatggtt ttgtcgatac
    11281 ggtacgtaat ctggcgctga tttgcgaaag cgatctgctc ggtgaacgcg ttgcccgtcg
    11341 tcgtcaggat tctcgccgca ccatcaaccc cgatacactg atccgtaacc tcgcggaact
    11401 gcatattggt cagccggtgg tccatctgga gcacggcgtc gggcgctatg ccggaatgac
    11461 cacgctggaa gcaggcggca ttaccggcga gtatctgatg ctcacctatg ccaacgacgc
    11521 caaattgtat gttccggtgt cgtcgctgca tctgattagc cgttacgcgg gtggtgcgga
    11581 agaaaatgcc ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc
    11641 ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc
    11701 caaagagggc ttcgcgttta aacacgatcg tgagcagtat cagttgttct gcgacagctt
    11761 cccgtttgaa accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg
    11821 tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt
    11881 ggcgatgcgc gccgctttcc tggcagtaga taaccacaag caggtggcgg tgctggtgcc
    11941 taccaccctt ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc
    12001 ggtacgtatc gaaatgctct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc
    12061 ggaagtggcg gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga
    12121 cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg
    12181 tcataaagag cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac
    12241 gccgatccca cgtacgctga atatggcaat gagcggaatg cgtgacctat cgattatcgc
    12301 cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt
    12361 ggtccgcgag gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa
    12421 tgatgtggaa aacattcaga aagccgccga acggctggca gaactggtgc cagaagcgcg
    12481 gatcgccatc ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt
    12541 ccatcatcaa cgtttcaacg tgctggtttg tacaaccatt atcgaaaccg ggatcgacat
    12601 cccgacagcc aacaccatta tcattgaacg cgcggatcac ttcggtctgg cgcaactgca
    12661 ccagttacgc ggtcgcgtcg gacgttcgca tcatcaggca tatgcatggc tgctgacgcc
    12721 gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga
    12781 agatctcggg gcaggttttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga
    12841 actgcttggc gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga
    12901 gttgctggaa aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct
    12961 caccagccag caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat
    13021 tcctgacgtg aatacgcgtc tgtcgttcta taaacgtatt gccagcgcca aaacggaaaa
    13081 cgaactggaa gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cggatccggc
    13141 gcgtaccctg ctggatattg cccgtctacg ccagcaagcg cagaaactgg ggatcaggaa
    13201 gctggaaggt aatgagaaag gcggcgtgat cgaatttgcc gagaagaatc acgttaatcc
    13261 ggcctggttg attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac
    13321 gcgcctgaaa tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca
    13381 gtttatgcgt gaactggaag aaaacgcgat cgcttaatgg taaatcgggg gcgtttctgc
    13441 gcccccatct gcaacattta caaattcttt gcacttccct gcactatccg acactgtcac
    13501 catccataat tcaggcttat ctgtttatta caataacctt atatttatta tggatttttg
    13561 gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg
    13621 gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg
    13681 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    13741 aaaaaatata acgtcggctt tctcgctcta ttacaggcta accccggcgt tgatccttac
    13801 gtaccgcgcg cgggcagcgt gttaacgatc ccgttgcaaa ccctacttcc agacgcgccg
    13861 cgcgaaggca ttgtgatcaa cattgcggag cttcgtctct attactaccc gccgggtaaa
    13921 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    13981 acaatggtga ccaccgtttc tgacaagcgt gcaaacccaa cctggacgcc aacggcaaac
    14041 attcgcgcgc ggtataaggc acaaggaatt gagttgcctg cggtagtgcc ggctggaccg
    14101 gataatccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    14161 cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    14221 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    14281 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    14341 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    14401 gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg
    14461 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaacgctg
    14521 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    14581 agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat
    14641 tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg
    14701 ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgatt gtaccgactt
    14761 tttgttggcc ttctggcgtt gactgaactt cgacagccgc aaagctggca aatgacatgg
    14821 agcttaaaat cgccgcagcg atgagggttt ttacgttttt cataatagtg gccttatgca
    14881 gatgaatgac gtcgcaagca attacttaac gatcgataac taaatgatag atg
//
LOCUS       DAZKIF010000113        14889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZKIF010000113 DAZKIF010000000
VERSION     DAZKIF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.114"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            140..382
                     /gene="yjbE"
                     /locus_tag="ACHOTW_003611"
     CDS             140..382
                     /gene="yjbE"
                     /locus_tag="ACHOTW_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP9845650.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            496..1134
                     /locus_tag="ACHOTW_003612"
     CDS             496..1134
                     /locus_tag="ACHOTW_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP9845651.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1131..1868
                     /locus_tag="ACHOTW_003613"
     CDS             1131..1868
                     /locus_tag="ACHOTW_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9845652.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLGGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1868..3964
                     /locus_tag="ACHOTW_003614"
     CDS             1868..3964
                     /locus_tag="ACHOTW_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9845653.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4011..4289)
                     /locus_tag="ACHOTW_003615"
     CDS             complement(4011..4289)
                     /locus_tag="ACHOTW_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845654.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4290..4433
                     /locus_tag="ACHOTW_003616"
     CDS             4290..4433
                     /locus_tag="ACHOTW_003616"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845655.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4559..4969
                     /gene="psiE"
                     /locus_tag="ACHOTW_003617"
     CDS             4559..4969
                     /gene="psiE"
                     /locus_tag="ACHOTW_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP9845656.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(5013..6488)
                     /gene="xylE"
                     /locus_tag="ACHOTW_003618"
     CDS             complement(5013..6488)
                     /gene="xylE"
                     /locus_tag="ACHOTW_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFP9845657.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6860..7750)
                     /gene="malG"
                     /locus_tag="ACHOTW_003619"
     CDS             complement(6860..7750)
                     /gene="malG"
                     /locus_tag="ACHOTW_003619"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP9845658.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7765..9309)
                     /gene="malF"
                     /locus_tag="ACHOTW_003620"
     CDS             complement(7765..9309)
                     /gene="malF"
                     /locus_tag="ACHOTW_003620"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP9845659.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9463..10653)
                     /gene="malE"
                     /locus_tag="ACHOTW_003621"
     CDS             complement(9463..10653)
                     /gene="malE"
                     /locus_tag="ACHOTW_003621"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP9845660.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11018..12133
                     /gene="malK"
                     /locus_tag="ACHOTW_003622"
     CDS             11018..12133
                     /gene="malK"
                     /locus_tag="ACHOTW_003622"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP9845661.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12205..13545
                     /gene="lamB"
                     /locus_tag="ACHOTW_003623"
     CDS             12205..13545
                     /gene="lamB"
                     /locus_tag="ACHOTW_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP9845662.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13696..14616
                     /gene="malM"
                     /locus_tag="ACHOTW_003624"
     CDS             13696..14616
                     /gene="malM"
                     /locus_tag="ACHOTW_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP9845663.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3666 a         3836 c         3926 g         3461 t
ORIGIN      
        1 tttgatattc attccaatta tcctaaaacc ccatctctaa ttccccgcgc cgtaattcgc
       61 atgctttagt tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat
      121 tcaatgaagg gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct
      181 tgcggcgact tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag ggtcggcagc
      241 aacgtcggtt tcggcgggga gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt
      301 gggtgtcgcg ctcgcggcaa ccggtggcgg tgatggttct aataccggga ccaccacaac
      361 cacgaccacc agtacccagt aataaagtat gtatccccaa aataattcga gtcattgcat
      421 ctgtggctag aagtatgaag ggatttacca taaccacact ccggtgtggt tattctgccc
      481 ctctggagaa gagtcgtgaa gcgacctgca ttcattctta tctgcctgct attacaggcc
      541 tgttcagcca cgactaaaga gctgggcaat tcactgtggg acagtctgtt cggcacgcca
      601 ggcgtacagc tgacggacga tgatattcaa aatatgccct acgccagcca gtacatgcag
      661 cttaatggtg ggccgcagtt gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa
      721 tgggtcactc aggatcaggc cactctcgtc acacaacatg gtcgtctggt gaagactttg
      781 cttggcggcg acaacctgat tgaagtgaat aaccttgccg ccgacccgct gattaaaccc
      841 gcacaaatca ttgatggcgc aacctggatg cgcacgatgg gctggaccga gtaccagcag
      901 gtacgctacg ccaccgcacg ctcagtcttc aaatgggatg gcaccggtac cgttaaggtc
      961 ggcagcgatg aaaccgcggt tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc
     1021 tggcataacc gctattggat cgacagcgga gggcaaattc gccagtcgga acagtatctc
     1081 ggcgcggatt atttcccggt gaaaaccact ctcatcaagg cggcaaaaca atgattaaac
     1141 aaactattgt cgcgttgctt ttgagtgtgg gagcgtcatc ggtcttcgcg gcaggaaccg
     1201 tcaaggtgtt cagcaatggc agcagtgagg ccaaaacgct aacgggcgca gagcatttaa
     1261 tcgatctggt aggccaaccg cggctggcaa acagctggtg gcctggtgcg gtgattagcg
     1321 aagagctggc aacggcggca gcattgcgtc agcagcaggc gttgctgaca cggctggcag
     1381 aacagggcgc agattccagc gccgacgatg ccgctgcgat taacgcctta cgccagcaaa
     1441 ttcaggcgtt gaaggtgacg ggcaggcaaa aaatcaatct tgatcccgat atcgtccgcg
     1501 ttgctgaacg cgctaacccg ccgttgcagg gcaactacac gctgtgggtc ggaccaccac
     1561 cgtccacggt cacgttgttc gggcttatca gccgtcctgg caatcagcca ttcactcccg
     1621 gtcgcgacgt ggcgagctat ctctccgggc aaagtctgct tggcggtgcg gatcgtagct
     1681 acgcgtgggt ggtttacccg gacggacgca cacaaaaagc gccggtggct tactggaaca
     1741 agcgtcacgt agagccgatg cccggcagca ttatttatgt tggcctcgcg gactccgtct
     1801 ggagtgagat gcctgatgcc cttaacgccg acattcttca gactctgacg cagcggatac
     1861 ctcaataatg aaaaaaagac atctgcttag cttactggcg ctgggcatta gcacagcttg
     1921 ctacggcgaa acatatcctg cgcccattgg tccgtcgcag tcggatttcg gtggcgtagg
     1981 attattacaa acgcccacgg ctcgcatggc gcgggaaggg gagttgagtc tgaactatcg
     2041 cgataacgat cagtaccgtt attactcagc ttcagtgcaa ctcttcccgt ggctggaaac
     2101 aacgctgcgc tacaccgacg tgcgcacccg gcagtacagc agcgtcgaag cgttctctgg
     2161 cgatcaaacg tataaagata aagccttcga tctcaaactg cgtttgtggg aagagagtta
     2221 ctggctgccg caagtggcgg ttggtgcgcg ggatatcggc ggtacggggc tgtttgatgc
     2281 ggaatatctt gttgccagca aagcctgggg gccgttcgat tttacgcttg gcctgggctg
     2341 gggatatttg ggcacaagcg gtaatgtgaa aaatccgctc tgttcagcca gtgataaata
     2401 ttgctatcgc gataacagct acaaacaggc gggatctatc gacggtagcc agatgttcca
     2461 cggtcctgcc tcactgtttg gcggcgtgga ataccagacg ccctggcaac cgctgcgcct
     2521 gaaactggag tatgaaggca ataattatca gcaggatttt gccggtaagc tggagcaaaa
     2581 aagtaagttt aacgtcgggg cgatttatcg cgttaccgat tgggccgacg ttaaccttag
     2641 ctatgaacgt gccaacacct ttatgtttgg cgtcacgctg cgcaccaact ttaacgatct
     2701 gcgcccgtct tacaacgata acgcccgccc gcaatatcaa ccgcagccgc aggatgccat
     2761 tttgcagcat tcggtggtgg cgaatcagtt aacgctgttg aaatacaatg ccggacttgc
     2821 cgatccgcag atccaggcga aaggcgatac gctgtatgtt accggcgagc aggtgaaata
     2881 tcgtgattcg cgcgaaggga tcgttcgtgc taatcggatc gtgatgaatg atctgccgga
     2941 tgggatcaag acgatccgcg ttacggaaaa tcgccttaac atgccgcagg tgacgacgga
     3001 aacggatgtc gccagcctga aaaatcatct cgccggagag ccgttgggcc acgaaacgaa
     3061 gctggcgcaa aaacgcgtcg agccagtggt tccgaagtcc accgagcagg gctggtatat
     3121 cgacaaatcg cgctttgatt tccatatcga tccggtgctg aaccagtcgg tcggtggccc
     3181 ggaaaacttt tacatgtatc agctgggcgt gatgggaacg gcagatttgt ggctgacgga
     3241 ccatctgctg accaccggca gcctgtttgc aaatcttgcc aacaactacg acaagtttaa
     3301 ctacaccaat ccgccgcagg actcgcactt accgcgcgtg cgtacccatg tgcgcgagta
     3361 tgtgcagaac gatgtctatg tgaataacct gcaagccaac tacttccagc atctgggcaa
     3421 cggcttctac ggtcaggtct acggaggtta tctcgaaacc atgtttggcg gtgcgggggc
     3481 agaagtgttg tatcgcccgc tggacagcaa ctgggcgttt ggtctggatg ccaactacgt
     3541 taaacagcgc gactggcgta gtgcaaagga tatgatgaaa ttcaccgact acagcgtgaa
     3601 aaccggacat ctgaccgcct actggacgcc atctttcgct caggatgtgt tagttaaagc
     3661 cagcgtcggg cagtatctgg caggggataa aggcggcacg ctggagatcg ctaaacgctt
     3721 tgacagcggc gtggtggtgg gcggctatgc cacgatcacc aacgtttcga aagaggagta
     3781 cggcgaaggg gacttcacca aaggcgtgta tgtctctgta ccgttggatc tcttctcgtc
     3841 tggcccgaca cgcagccgtg cggcgattgg ctggacgccg ctgacgcgtg acggtggtca
     3901 gcaacttggg cgtaagttcc agttgtatga catgaccagc gaccgtagcg tcaatttccg
     3961 ctaagtcatg ggaaaggtgc cagttttcgc aatcactggc acctttattc ttaatggcct
     4021 gttgagccat caggaacgca gttcaccccc ggtttgcgat ctggtgtgta aaggcgaaaa
     4081 cattctgtct tcctgtccgt cgatatcggt tgcaggtctg gttgcctgag cgactgggcc
     4141 atcattgggc tggagacatt gctttgcggt aggtcggctt tatcagcagt accgagcgga
     4201 ccagcataag caggaagagc agagactgat aacatcaaag cagcaaaata aggcttcatt
     4261 tttaccacct ttatcaggtt acgtttcatt tgttccagag gaacattgtc gatttttcgc
     4321 gcattgctgg tggctgggaa tcacctgaat gggtgatttt tgaattaccg gctttggtgc
     4381 ggtttgtctt gccggatgcg ccgccaggcg cggcttatcc ggcctacggg taggtatatc
     4441 cggctttggt ggaggcgcgc tccaaatcca ggttgaacaa aacatacaca aaaaatatag
     4501 atctccgtca catttttgcg ttatacagga agctcgccac tgtgaaggag gtactgctat
     4561 gacgtcactc tctcgtccgc gcgtggagtt tatctccacc attttgcaga ccgtactcaa
     4621 tcttggcctg ctgtgcctcg gccttatttt ggttgtcttc ctcggcaaag aaacggtgca
     4681 tctggctgat gtgctgttcg cgccagaaca agccagcaaa tatgagctgg tagaaggact
     4741 ggtggtttac tttctctatt tcgaatttat cgcgctgatt gtgaagtact ttcagtccgg
     4801 ttttcacttc ccgctacgtt actttgtcta tattgggatc accgccattg tgcgcttgat
     4861 catcgtcgat cataaatcgc cactggatgt gttgatctac tcggctgcga tcctgttact
     4921 ggtggtcacc ctgtggctgt gtaattcgaa acggctgaag cgggagtaaa aagacagcac
     4981 gccgaactgg cgcggcgtgc tggacaggaa gattacagcg tagcagtttg ttgtgttttc
     5041 ttcgtttccg gttcccagag ggcttccagc tcctcaaggg ttttaccttt ggtttccggg
     5101 acaaatttcc acataaacag tgctgccaga acgcccatac aaccgtaaat ccagtaggag
     5161 aaaccgttgt ggaaatgggc caccagccag gagtttttgt ccatcatcgg gaaggtccag
     5221 gagacgaagt agttcgccag ccactgggcc gccaccgcga ttgccagcgc tttacctcga
     5281 atagcattcg ggaagatttc cgacagcagt acccagcata ccggacccca ggacatggca
     5341 aaggcggcaa catagaacag catcgacagt agcgccacaa tacccggtgc ctgagtgtaa
     5401 aacgcggtac cgaggctaaa cataccgatt gccattccga gtgcgccgat aatttgcagt
     5461 ggcttacgac caaatttatc caccgtcata attgccagaa cggtgaaggt gaggttgata
     5521 actccgacaa taatggtctg caacagcgcg atatccgtgc tggcccccag cgttttgaac
     5581 acttccggcg cgtagtacag caccacattg atgccgacaa attgctggaa gatggagagc
     5641 ataacgccga tgacaatcac gcccacgcca aacatcagca gacgaccacc ggttttgcgg
     5701 ccatgatcca gggagtgttt aatttcctgt actgcctgag ttgcaagcgt gttgcccata
     5761 attttgcgca ggataccttc cgcctgttct tgcttgccgc gtgacatcag ccagcgagga
     5821 ctttctggca cggtatacag cagcattaag aacagcagtg cagggataca ttccgaggca
     5881 aacatataac gccagccgtc agtattcagc cagctggcat caccggaacg cgcaataaaa
     5941 tagtttacgc agtaaactaa aagctgcccg aaaataatcg caaactggtt aaatgaaacc
     6001 agtttcccgc gaatatgagc aggagcaagt tccgcaatat acattggtga aagcattgag
     6061 gctaaaccaa cgccaatacc gccaataata cgataaataa caaattccgg gacataacct
     6121 gccagataaa caggcactgt gttgtccggg tttatagagg taaaaccaag ttctggccag
     6181 gcagaaccta caccagaaat aaaaaacagg acagcagcaa tcttaagtga atcacgacga
     6241 ccgaagcggt tactgcaata accaccgagg gcaccgccga tgatgcaacc aatcagagcg
     6301 ctggccacgc aaaaccctaa cagggagttg gcagcggatt cacttaagtt ttgtggagca
     6361 acaaagacgg tattgagtga ctcaacagta ccggaaataa cggcggtgtc gtagccaaat
     6421 aataaaccac ctaatgtagc gactaaggta atcgaaaata tataactgga attatactgg
     6481 gtattcattc agacctgcct tagaccattc tgatctttta tcagtgcatt gatgttggaa
     6541 ttgtcaggca gttaaggcaa ttatcatttt ttacaatgcg tttacgtaat gtcttttctg
     6601 tgatcttaat tgtgataatt atccaaaatt gataaaaaca atactattgc cgtgactcag
     6661 agcacgaaag agaattatcg taagtgggaa aacaaataac gtaaaaataa taagctctat
     6721 gatggaaata ttaaccggcg aacgattcag attgcagacg aaagaaaaaa aggcgctccg
     6781 tggagcgccg aataacagtc acaagttggg ataacgtaag ttgagggtgc agcggcataa
     6841 cattggcaga acaacatctt taacctttca caccacctgc cgtcaggccg ttgaccagcc
     6901 agcgttgagc cagcaagaag acgatggtga tcggtaatgc agacatcacg gcagcggcgg
     6961 caaagtcacc ccacaggtag ttttgcggat tgaggtattg ctgcatcccc acggccaggg
     7021 tgtagctgtt tacgtcacgc agtaacagcg acgcgaccgg aacttcagta atggcagcga
     7081 taaacgacag gatgaatacc accgccagaa tcggtactga cagcggcaac aggacaaggc
     7141 ggaaggcctg ccacggtgtc gcaccatcca gcgcagccgc ttcttccagc gaactgtcga
     7201 tggtttcgaa atagcctttg atggtccaga cgtgcagcgc aatcccaccc agatacgcga
     7261 aaattacgcc gccgtgagta ttcaggccaa tgaatggaat gtactcaccc agacgatcaa
     7321 acaacgcata caacgcgacc agtgaaagta ctgccgggaa catctggaaa atcagcattc
     7381 ctttcagcag cgtcgctttg cctggaaagc gcatacgggc gaaagcgtag gcgcaggtgg
     7441 tggagagcgc cacaatgcca atcgcggaaa tcccggcgac ctttaccgag ttccacagcc
     7501 acagcagcac cgggaatggc ggtggcgtaa tgcgaccatc agcctgttcc acgctaaaac
     7561 ctaatgccag tttccagtga tcccaggaga tttgctccgg gatcaggctg ccggtcgcaa
     7621 agttcccctg gcgcagtgag atagcgacga ccatcagcag cgggaacata atcgccgcga
     7681 taaaaagtag cagtagcagg tgagtaataa acaaacgtgc tttttgcgat ttcggttgga
     7741 ccattgccat ttttgttatc tcccttaatc aaacttcatt cgcgtggctt tcaggttcac
     7801 tatcgccagc gcacccacca gcaggaagat cagcgtggca attgctgctg ccagaccgaa
     7861 gtcctgaccc ccgccgcctt caaaagcgat gcggtaggtg tagttaacaa gcaggtcggt
     7921 ataaccggct ggcgtggtcg tgccaagacg atccgggccg ccgttggtta acagttgaat
     7981 cagcacgaag ttgttaaagt taaaggcgaa gctggcgatc atcagcggcg tcagcggttt
     8041 aatcagcagc ggcagcgtaa tcttaaagaa gttctggaac ggacctgcgc catccattgc
     8101 tgaggcttca tacaaatcgt ccggaatcgc tttcagcaag cccatgcaga ggatcatcat
     8161 gtacggatag cccagccagg tattgacgat aatcagcatc gttcgggcgg tggtcggatc
     8221 gctgaaccag gcgggcttca cgccaaacag cgcgctcaac atcatgttga tctcaccgaa
     8281 gctctggtta aacaaccctt tgaaaatcaa gattgaaatg aacgatggca ccgcgtaggg
     8341 cagaatcagc aggacgcgat agaccgcttt gccgcgcaac gcttcccact gcaccagaca
     8401 cgccagaacc atgccgactg ccaccgttaa aaagacagtg atcagcgaga acaccacggt
     8461 ccagacgaaa atggcgagga acggtttctg aatgccttcg tcggtgaaga cgcgagtaaa
     8521 gtttttccag ccggtggtca cggtgtaacc ggggcttagc ttttcatcac cccagttgcc
     8581 gtcggcggta atggactggt aaaagccaat ttggttattc ggacgatatt tcacgccgct
     8641 ctgattattc gtcaacgtgc cgtcaccgtc gagtgtgtag agcggctgcg tgccagaaaa
     8701 ctggcgcagg gagctcatca tcactttgtt gccatccggc agaatggcgg taatgtcact
     8761 cagcgcctga cgattctggg taatcacgcg tagatttgcg cgttcgcctt cgggctgggc
     8821 ggtcgtttct ttcagttgca gtttttgctc tctgccaaat ttaaaagcgt cggagaggta
     8881 atttttgccg gtttcgccgt cgctgagcgc cagttgccac tcatcgcccg ccgggtaaag
     8941 accaaagtta taggttttgc ctgcttgcca ggagcgatct aacaacactt cctgtgcacg
     9001 ttcaaaagtc agctggttag tgctgctgta gttggtgaag gcaatggcga tggtgcagac
     9061 cagagggaag aggacgaata atcccattcc agccattccc gggtaaacat agcgccaggc
     9121 gtaggcttta cgattggcga aaatatacag ccccgctgaa ctcaatatca gcgtggtaat
     9181 ggcgaacagg tattcccctt gtgcgtacat taaaacaaca aggtaaccca ccagcaggcc
     9241 gagcagacct aacactgacc atttcagcgc gtcgctttgc caccaatgtt tctttttaat
     9301 gacatccatg gggttcttcc tcattccagg acggataagg ctttcatgcc ttatccgaca
     9361 acaactgcct gatgcgacgc tgacgcgtct tatcaggcct acatacgttt cggttttgta
     9421 ggccggacaa ggcgttcacg ccgcatccgg catttcacag cattacttgg tgatacgagt
     9481 ctgcgcgtct ttcagggctt catcgacagt ctgacgaccg ctggcggcgt tgatcaccgc
     9541 agtacgcacg gcataccaga aagcggacat ctgcgggatg ttcggcatga tttcaccttt
     9601 ctgggcgttt tccatggtgg ctgcaatacg tggatctttc gccaactctt cctcgtaaga
     9661 cttcagcgct acggcaccca gcggtttgtc tttattaacc gcttccagac cttcgtcagt
     9721 cagcagatag ttttcgagga actctttcgc cagctctttg ttcggactgg cggcgttaat
     9781 accggcgctc agcacgccaa cgaacggttt agatggttga cccttgaagg tcggcagtac
     9841 cgttacacca taattcactt tgctggtgtc gatgttggac catgcccacg ggccgttgat
     9901 agtcatcgct gtttcgcctt tattaaaggc agcttctgcg atggagtaat cggtgtctgc
     9961 attcatgtgt ttgtttttaa tcaggtcaac caggaaggtc agacccgctt tcgcgccagc
    10021 gttatccacg cccacgtctt taatgtcgta cttgccgttt tcatacttga acgcataacc
    10081 cccgtcagca gcaatcagcg gccaggtgaa gtacggttct tgcaggttga acatcagcgc
    10141 gctcttacct ttcgctttca gttctttatc cagcgccggg atctcttccc aggtttttgg
    10201 cgggttcggt agcagatctt tgttataaat cagcgataac gcttcaacag cgatcgggta
    10261 agcaatcagc ttgccgttgt aacgtacggc atcccaggta aacggataca gcttgtcctg
    10321 gaacgctttg tccggggtga tttcagccaa caggccagat tgagcgtagc caccaaagcg
    10381 gtcgtgcgcc cagaagataa tgtcagggcc atcgccagtt gccgcaacct gagggaattt
    10441 ctcttccagt ttatccggat gctcaacggt gactttaatt ccggtatctt tctcgaattt
    10501 cttaccgact tcagcgagac cgttatagcc tttatcgccg ttaatccaga ttaccagttt
    10561 accttcttcg attttggcga gagccgaggc ggaaaacatc atcgtcgtta atgcggataa
    10621 tgcgaggatg cgtgcacctg tttttatttt cataatctat ggtccttgtt ggtgaagtgc
    10681 tcgtgaaaac acctaaacgg actctagttt ctttatacgg caacctcttt ccatcctcct
    10741 tgcccctacg ccccaccgtt gctttgtgtg atctctgtta cagaattggc agtaatgtgg
    10801 agatgcgcac ataaaatcgc cacgattttt gcaagcaaca tcacgaaatt ccttacatga
    10861 cctcggttta gttcacagaa gccgcgttct catcctcccg cctcctcccc cataaaaaag
    10921 ctagggggtg gaggatttaa gccatctcct gatgacgcat agtcagccca tcatgaatgt
    10981 tgctgtcgat gacaggttgt tacaaaggga gaagggcatg gcgagcgtac agctgcaaaa
    11041 tgtaacgaaa gcctggggcg aggtcgtggt atcgaaagat atcaatctcg atatccatga
    11101 aggtgaattc gtggtgtttg tcggaccgtc tggctgcggt aaatcgactt tactgcgcat
    11161 gattgccggg cttgagacga tcaccagcgg cgacctgttc atcggtgaga aacggatgaa
    11221 tgacactccg ccagcagaac gtggcgttgg tatggtgttt cagtcctacg cgctctatcc
    11281 ccacctgtca gtagcagaaa acatgtcatt tggcctgaaa ctggcaggcg caaaaaaaga
    11341 ggtgattaac caacgcgtca accaggtggc ggaagtgcta caactggcgc atttgctgga
    11401 tcgcaaaccg aaagcgctct ccggtggtca gcgtcagcgt gtggcgattg gccgtacgct
    11461 ggtggccgag ccaagcgtat ttttgctcga tgaaccgctc tccaacctcg atgctgcact
    11521 gcgtgtgcaa atgcgtatcg aaatctcccg tctgcataaa cgcctgggcc gcacaatgat
    11581 ttacgtcacc cacgatcagg tcgaagcgat gacgctggcc gacaaaatcg tggtgctgga
    11641 cgccggtcgc gtggcgcagg ttgggaaacc gctggagctg taccactatc cggcagaccg
    11701 ttttgtcgcc ggatttatcg gttcgccaaa gatgaacttc ctgccggtaa aagtgaccgc
    11761 caccgcaatc gatcaagtgc aggtggagct gccgatgcca aatcgtcagc aagtctggct
    11821 gccagttgaa agccgtgatg tccaggttgg agccaatatg tcgctgggta ttcgcccgga
    11881 acatctactg ccgagtgata tcgctgacgt catccttgag ggtgaagttc aggtcgtcga
    11941 gcaactcggc aacgaaaccc aaatccatat ccagatccct tccattcgtc aaaacctggt
    12001 gtaccgccag aacgacgtgg tgttggtaga agaaggtgcc acattcgcta tcggcctgcc
    12061 gccagagcgt tgccatctgt tccgtgagga tggcactgca tgtcgtcgac tgcataagga
    12121 gccgggcgtt taagcacccc acaaaacaca caaagcctgt cacaggtgat gtgaaaaaag
    12181 aaaagcaatg actcaggaga tagaatgatg attactctgc gcaaacttcc tctggcggtt
    12241 gccgtcgcag cgggcgtaat gtctgctcag gcaatggctg ttgatttcca cggctatgca
    12301 cgttccggta ttggctggac aggtagcggc ggtgaacaac agtgtttcca gactaccggt
    12361 gctcaaagta aataccgtct tggcaacgaa tgtgaaactt atgctgaatt aaaattgggt
    12421 caggaagtgt ggaaagaggg cgataagagc ttctatttcg acactaacgt ggcctattcc
    12481 gtcgcgcaac agaatgactg ggaagctacc gacccggcct tccgtgaagc aaacgtgcag
    12541 ggtaaaaacc tgatcgaatg gctgccaggc tccaccatct gggcaggtaa gcgcttctac
    12601 caacgtcatg acgttcatat gatcgacttc tactactggg atatttctgg tcctggtgcc
    12661 ggtctggaaa acatcgatgt tggcttcggt aaactctctc tggcagcaac ccgctcctct
    12721 gaagcaggtg gttcttcctc tttcgccagc aacaatattt atgactatac caacgaaacc
    12781 gcgaacgacg ttttcgatgt gcgtttagcg cagatggaag tcaacccggg cggcacatta
    12841 gaactgggtg tcgactacgg tcgtgccaac ctgcgtgata actatcgtct ggttgatggc
    12901 gcatcgaaag acggctggtt attcactgct gaacatactc agagtgtcct gaagggcttt
    12961 aacaagtttg ttgttcagta cgctactgac tcgatgacct cgcagggtaa aggtctgtcg
    13021 cagggttctg gcgttgcatt tgataacgaa aaatttgcct acaatatcaa caacaacggt
    13081 cacatgctgc gtatcctcga ccacggtgcg atctccatgg gcgacaactg ggacatgatg
    13141 tacgtgggta tgtaccagga tatcaactgg gataacgaca acggcaccaa gtggtggacc
    13201 gtcggtattc gcccgatgta caagtggacg ccaatcatga gcaccgtgat ggaaatcggc
    13261 tacgacaacg tcgaatccca gcgcaccggc gacaagaaca atcagtacaa aattaccctc
    13321 gcacaacaat ggcaggctgg cgacagcatc tggtcacgcc cggctattcg tgtcttcgca
    13381 acctacgcca agtgggatga gaaatggggt tacgactaca acggcgatag caaggttaac
    13441 ccgaactacg gcaaagccgt tcctgctgat ttcaacggcg gcagcttcgg tcgtggcgac
    13501 agcgacgagt ggaccttcgg tgcccagatg gaaatctggt ggtaatagca aaacctgggc
    13561 cggataaggc gtttacgccg catccggcat aaaacaggtt gtcattatct gaaaggggcg
    13621 aaagcccctc tgattatcgg gtttagcgcg ctattgcctg gctaccgctg agctccagat
    13681 tttgaggtga aaacaatgaa aatgaataaa agtctcatcg ccctctgttt atcagcaggg
    13741 ttactggcaa gcgcgcctgg aatcagcctt gccgatgtta actacgtacc gcaaaacacc
    13801 agcgacgcgc cagccattcc atctgccgcg ctgcaacaac tcacctggac accggtcgat
    13861 caatctaaaa cccagaccac ccaactggcg accggcggcc aacaactgaa cgttcccggc
    13921 atcagtggtc cggttgctgc gtacagcgtc ccggcaaaca ttggcgaact gaccctgacg
    13981 ctgaccagcg aagtgaacaa acaaaccagc gtttttgcgc cgaacgtgct gattcttgat
    14041 cagaacatga ccccatcagc cttcttcccc agcagttatt tcacctacca ggaaccaggc
    14101 gtgatgagtg cagatcggct ggaaggcgtt atgcgcctga caccggcgtt ggggcagcaa
    14161 aaactttatg ttctggtctt taccacggaa aaagatctcc agcagacgac ccaactgctc
    14221 gacccggcta aagcctatgc caaaggcgtt ggtaactcga tcccggatat tcccgatccg
    14281 gttgctcgtc ataccaccga tggcttactg aaactgaaag tgaaaacgaa ctccagctcc
    14341 agcgtgttgg tcgggccttt atttggttct tccgctccag ctccggttac ggtaggcaac
    14401 acggcggcac cagctgtggc tgcacccgct ccggtgccgg tgaagaaaag cgagccgatg
    14461 ctcaacgaca cggaaagtta ttttaatacc gcgatcaaaa acgctgtcgc gaaaggtgat
    14521 gttgataagg cgttaaaact gcttgatgaa gctgaacgcc tgggatcgac atctgcccgt
    14581 tccaccttta tcagcagtgt aaaaggcaag gggtaattac gccccacagt gctgattttg
    14641 caacaactgg tgcgtctcct ggcgcacctt tttttatgct tcattccagg gatatgagcg
    14701 attttttata gtaactcact tcttcttcac taagaatatc cattatctca atgtattatc
    14761 agagattctt ttcctttcgc cggtagtgtc tggacgctca ggctactttt tccagttatt
    14821 tcatttctgt aatgcagagg ttttatgtta ggtcatatct caaagtttga cggcaataac
    14881 tctttgata
//
LOCUS       DAZKIF010000114        14758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZKIF010000114 DAZKIF010000000
VERSION     DAZKIF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.115"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            215..1558
                     /locus_tag="ACHOTW_003625"
     CDS             215..1558
                     /locus_tag="ACHOTW_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845664.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1707..3215)
                     /gene="putP"
                     /locus_tag="ACHOTW_003626"
     CDS             complement(1707..3215)
                     /gene="putP"
                     /locus_tag="ACHOTW_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP9845665.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3374..3583)
                     /locus_tag="ACHOTW_003627"
     CDS             complement(3374..3583)
                     /locus_tag="ACHOTW_003627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845666.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3638..7600
                     /gene="putA"
                     /locus_tag="ACHOTW_003628"
     CDS             3638..7600
                     /gene="putA"
                     /locus_tag="ACHOTW_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9845667.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7640..8278)
                     /gene="rutR"
                     /locus_tag="ACHOTW_003629"
     CDS             complement(7640..8278)
                     /gene="rutR"
                     /locus_tag="ACHOTW_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP9845668.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8566..9657
                     /gene="rutA"
                     /locus_tag="ACHOTW_003630"
     CDS             8566..9657
                     /gene="rutA"
                     /locus_tag="ACHOTW_003630"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP9845669.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9657..10349
                     /gene="rutB"
                     /locus_tag="ACHOTW_003631"
     CDS             9657..10349
                     /gene="rutB"
                     /locus_tag="ACHOTW_003631"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP9845670.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10361..10747
                     /gene="rutC"
                     /locus_tag="ACHOTW_003632"
     CDS             10361..10747
                     /gene="rutC"
                     /locus_tag="ACHOTW_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP9845671.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINETYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10755..11555
                     /gene="rutD"
                     /locus_tag="ACHOTW_003633"
     CDS             10755..11555
                     /gene="rutD"
                     /locus_tag="ACHOTW_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP9845672.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11565..12155
                     /gene="rutE"
                     /locus_tag="ACHOTW_003634"
     CDS             11565..12155
                     /gene="rutE"
                     /locus_tag="ACHOTW_003634"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP9845673.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12166..12660
                     /gene="rutF"
                     /locus_tag="ACHOTW_003635"
     CDS             12166..12660
                     /gene="rutF"
                     /locus_tag="ACHOTW_003635"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP9845674.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQN
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12681..14009
                     /gene="rutG"
                     /locus_tag="ACHOTW_003636"
     CDS             12681..14009
                     /gene="rutG"
                     /locus_tag="ACHOTW_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP9845675.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14092..14199)
                     /locus_tag="ACHOTW_003637"
     CDS             complement(14092..14199)
                     /locus_tag="ACHOTW_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFP9845676.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
BASE COUNT         3492 a         3959 c         3924 g         3383 t
ORIGIN      
        1 accccgatgg cagcattgta aattcgccaa taactggcat tcctcctgct ttttacgggt
       61 gtttccatta acaccagaat gagcggattg tttactacat caagaacgca tccgaagtgt
      121 acatcctggt gcgccgactg aatcgcccgg agccatgcgg atggctacga cttaatacga
      181 atcattaacc ctgtaaggat acggagagca ttacatgaga ccaatatcca acctcaatac
      241 gagcgcagca acctatattc caccgcaaca actcccgagt agttgtctgg aaactttgac
      301 gcttctggtg cagatgaata attatatcaa aagggatgcg gactacagca cgggcatggc
      361 tgtagctcca ctggttcctg aagaggttca cttgctggct gctgcgatga caaccgaact
      421 gcatcgacat cagttccaac cggtggtaag tgcaaatgat ctgcaacttc ctgaaccgtt
      481 caccttcgat gttgccggtt tcaccatcac cttcacgaag acgcaagagc atgacaacac
      541 gggcaatctc atgaaaattg cagtcagcaa gagcggtgtg tgcaccagta caaatattac
      601 tcttgagctg tttcactcca ttgtcacaac cctgatgtca cgcagtcaat atggaacctt
      661 tgatctctgg tccgtacggc caatactcac ggatgaaagt caaaacaggg tgcatgaagc
      721 agctcgctac tccccggccc agcaatacgg aagagaagaa acacacttta ccaacaatgg
      781 catgcgagaa tttggaaata tgtctccgct ggcgtggcgt aacgatgtgg aattacagca
      841 atcgtgtaat acaagcaaca tcccaccaaa tgctgcaaat gacgacataa acaacaccag
      901 gcagaccatc gaggaccagc cagaggcgga cgagccacag caatatgtta aattgactgt
      961 agacgacatg cgaaaatggg cggctatgga ccagcaggcg cgaaacgctc tacagggagt
     1021 ttcaggatgg tgtacgcgca atcattttaa tatcaaaaat gccagaaatt acctgaccga
     1081 tcacggcctg aactacgcgg gacaggtaaa ggtaaacagg ccgcatgaat atgccaaatt
     1141 cacccttgaa catatccgcc agtgggcagc cttaaacaag catgtgcgaa aaccggttgg
     1201 ttatttagaa aagtggtgta aggaaagaaa cctcgcgcca actactgcca ggaattacct
     1261 gaagaacgac gggcttaccg ccctgggtga gttgaagctg acagggccgc agaaatgggt
     1321 cacgttcacc ttcggagaca tcgtacaatg ggcgaatatg acccaggagg agcgtaacag
     1381 cgcaggggga gccgaaaagt ggagtaaaaa acgcggcttc cagtggtcta ctgccagatc
     1441 ctacctgaaa tcgagcggag tgaccaagca aggtgcgaga aaactggcgt ggttaaaaaa
     1501 cagcgggaat atgtcgaatc cattctatct cgcccaacca accagccgca ggacataagc
     1561 tgaaggtacc gatagcggca attttgttca tctgtgccgg gtgggcaaat agcaattgcc
     1621 cccgaacact ccctgccagg caacttatac cgcatggcag ggaacattcg ggttaaaaag
     1681 caaaagataa ctaattactc ttcagcttag ccttcctgca accgtgacgg cggagccgaa
     1741 tgatagtgcg catcggcctc ggcaaagcgt ttttgcatcg ccgctgacgg tgctttaccc
     1801 agcaaactaa acactacaat cccaatactg ccaaagataa agcccggaat aatttcgtac
     1861 agtcccagcc agccgaactg tttccagacg atcaccgtca gcgcaccgat gatcatcccc
     1921 gccagcgcac cgttgcgcgt catgcgtgac cacatcaccg aaaacagcac caccggacca
     1981 aacgccgcgc caaagcctgc ccacgcgtag ctcactaagc ccagcacgcg gttttccggg
     2041 ttcgctgcca acgcaatcgc caccagcgcc accaccagca ccatcacacg ccctacccac
     2101 accagttctt tctggctggc atgtttacgc agaaacgctt tgtacaaatc ttcggtaatc
     2161 gcactggaac ataccagcag ctggcaactt aacgttgaca ttaccgccgc cagaatcgcc
     2221 gacagcagaa tcccggcaat ccacgggtta aacagaattt gcgccagttc gataaacacg
     2281 cgctcggcgt tctggtttac cgcaccagct accgacgggt gctcgttaaa gtaagcgata
     2341 ccaaagaagc cgacagccac cgcccctgcc aggcagagga tcatccaggt catactgata
     2401 cgacgcgcat ggacgatgct gtggtgagaa tccgccgcca taaaacgcgc caggatgtgc
     2461 ggttgcccga agtaacccag cccccacccc atcagcgaga taatggcgac aaagttcagc
     2521 cctttgagca tatccacgtt ttcgatgctc ttttgtttga tcacttccag cgagtcacca
     2581 aagccaccga cactgataat gacgataacc ggcgtcagga tcagggcaaa aatcatcagg
     2641 ctggcctgta cagtgtcagt ccagctcacc gcgaggaaac cgccaataaa ggtgtaaagg
     2701 atcgtcgccg cagctcccgc ccacagagcc gtttcgtagc tcatgccaaa ggtactttca
     2761 aacagacgcg cgcctgccac aatgcccgaa gcgcaataaa tggtgaagaa cagcaaaatc
     2821 accagcgcgg agataatgcg caaaatgcgg cttttatctt caaaacgccc ggtgaaataa
     2881 tccggtaatg ttaaggcgtt attgttgtat tcggtatgca cacgcaaccg cccggccacc
     2941 agcttccagt taatccacgc gcctaatgtc aggccaatgg cgatccaact ttcggaaatc
     3001 ccggaaagaa aaaccgcgcc cggcaacccc attaacagcc agccgctcat atccgatgca
     3061 cccgccgata atgccgtcac gaatggccca aggctacgtc cgcccagaat atagtcgtca
     3121 aagtttttcg ttgatcgcca ggcgataaac ccaatcaata tcatgccaaa gatatagaca
     3181 caaaatgtca ccaacatcgg tgtgctaata gccatctaaa gtctccaaaa aattattatc
     3241 ggcaatgtcg aaacttgccg ttatatctgc caccggaacg gggtaacaga gtttatgttt
     3301 taccagggcg accgtatcct gccggaagcg ctggttattc acaatcgatt taacacacca
     3361 tttacattaa attttagtgc tcagcgacac tatttttcat caggttgcac tctctcacat
     3421 tttttgcggt tgcacctttc aaaaatgtta actgccgcag agaaaaagtc tgagttattt
     3481 ttttaatccc tgtcatatcg atttctttta ttaacatttc attcattttt aagcttgcta
     3541 cgcaggtcac atttaacatg gttgcacaaa gttgcaacat catggatatt tcacgataac
     3601 gttaagttgc acctttcaga acaacaggag taatggcatg ggaaccacca ccatgggggt
     3661 taagctggac gacgcgacgc gtgagcgtat taagtctgcc gcgacacgta tcgatcgcac
     3721 gccacactgg ttaattaagc aggcgatttt ttcttatctc gaacaactgg aaaacagcga
     3781 tactctgcca gagctacctg cgctgctttc tggcgctgcc aatgagagcg atgaagcacc
     3841 gactccggca gaggaaccac accagccatt cctcgacttt gccgagcaaa ttttgcccca
     3901 gtcggtttcc cgcgctgcga tcaccgcggc ctatcgccgc ccggaaaccg aagcggtttc
     3961 tatgctgctg gaacaagccc gcctgccgca accagttgct gaacaggcac acaaactggc
     4021 gtatcaactg gccgataaac tgcgtaatca aaaaaatgcc agtggccgcg caggtatggt
     4081 ccaggggtta ttgcaggagt tttcgctgtc atcgcaggaa ggcgtggcgc tgatgtgtct
     4141 ggcagaagcg ttgttgcgta ttcccgacaa agccacccgt gatgcgttaa ttcgcgacaa
     4201 aatcagcaac ggtaactggc agtcacacat tggtcgtagc ccgtcactgt ttgttaatgc
     4261 cgctacctgg gggctgttgt ttactggcaa actggtttcc acccataacg aagccagcct
     4321 ctcccgctcg ctgaaccgca ttatcggtaa gagcggtgaa ccgctgatcc gcaaaggtgt
     4381 ggatatggca atgcgcctga tgggcgagca gttcgtcacg ggcgaaacca tcgcggaagc
     4441 gttagccaat gcccgcaagc tggaagagaa aggtttccgt tactcttacg atatgctcgg
     4501 cgaagctgca ctgactgccg cagacgcaca ggcgtacatg gtttcctatc agcaggcgat
     4561 tcacgccatt ggtaaagcgt ctaacggtcg tggcatctat gaaggtccgg gcatttcaat
     4621 caagctgtcg gcgctgcatc cgcgttacag ccgcgcccag tatgaccggg taatggaaga
     4681 gctttacccg cgtctgaaat cactcaccct gctggcgcgt cagtacgata ttggtatcaa
     4741 cattgacgcc gaagaggccg atcgtctgga gatctccctc gatctgctgg aaaaactctg
     4801 tttcgagcca gagctggcag gctggaacgg catcggtttt gttattcagg cttatcaaaa
     4861 acgctgcccg ctggtgattg attacctgat tgatctcgcc acccgcagcc gtcgccgtct
     4921 tatgattcgc ctggtgaaag gcgcgtactg ggatagtgaa atcaagcgcg cgcagatgga
     4981 cggccttgaa ggttatccgg tttatacccg caaggtatat accgacgttt cttatctcgc
     5041 ctgtgcgaaa aagctgctgg cggtgccgaa tctaatctac ccgcagttcg caacgcacaa
     5101 cgcccacacg ctggcggcga tttatcaact ggcaggtcag aactactatc cgggtcagta
     5161 cgagttccag tgcctgcatg gtatgggcga gccgctgtat gagcaggtca ccgggaaagt
     5221 tgccgacggc aaacttaacc gtccgtgtcg tatttatgcc ccggtcggca cacatgaaac
     5281 gctgctggcg tatctggtgc gccgcctgct ggaaaacggt gctaacacct cgtttgttaa
     5341 ccgtattgcc gatacctctt tgccactgga tgaactggtc gccgatccgg tcactgctgt
     5401 agaaaaactg gcacaacagg aagggcaaac tggattaccg catccgaaaa ttcccctgcc
     5461 gcgcgatctt tacggtcacg ggcgcgacaa ctcggcaggg ctggatctcg ctaacgaaca
     5521 ccgtctggca tccctctctt ctgccctgct caatagtgca ctgcaaaaat ggcaggcctt
     5581 gccaatgctg gaacaaccgg tagcggcagg tgagatgtcg cccgttatta acccggcgga
     5641 accgaaagat attgtgggct atgtacgtga agccacgccg cgtgaagtag agcaggcgct
     5701 ggaaagtgcg gttaataacg cgccaatctg gtttgccact cctccggctg aacgcgcggc
     5761 gattttgcat cgcgctgccg tgctgatgga aagccagatg cagcaactga ttggtattct
     5821 ggtgcgtgaa gccggaaaaa ccttcagtaa cgccattgcc gaagtgcgtg aagcggtcga
     5881 ttttctccac tactacgccg gacaggtgcg ggatgatttc gctaacgaaa cccatcgtcc
     5941 attagggcct gtggtatgta tcagcccgtg gaacttcccg ctggcgattt tcaccgggca
     6001 gatcgctgcc gcactggcgg caggtaacag cgtactggca aaaccggcag aacaaacgcc
     6061 actgattgcc gcgcaaggga tcgccatttt gctggaagcg ggcgtaccgc caggcgtggt
     6121 gcaattgctg ccaggtcggg gtgaaaccgt gggcgcgcaa ctgacgggtg atgatcgcgt
     6181 acgcggggtg atgtttaccg gttcaaccga agtcgctacg ttactgcagc gcaatatcgc
     6241 cagccgcctc gacgctcagg gtcgccctat tccgctcatc gctgaaaccg gcggcatgaa
     6301 cgcgatgatt gtcgattctt cagcactgac cgaacaagtc gtaatagatg tgctggcctc
     6361 ggcgttcgac agtgcgggtc agcgttgttc ggcgctgcgc gtgctgtgcc tgcaagatga
     6421 gattgccgac cacacgttga aaatgctgcg cggcgcaatg gccgaatgcc ggatgggtaa
     6481 tccgggtcgc ctgaccaccg atatcggtcc ggtgattgat agcgaagcga aagccaatat
     6541 tgagcgccat attcagacca tgcgtagcaa aggccgtccg gtgttccagg cggtgcggga
     6601 aaacagcgaa gatgcccgtg aatggcaaag cggcaccttt gtcgccccga cgctgatcga
     6661 actggatgac tttgccgaat tgcaaaaaga ggtctttggt ccggtgctgc atgtggtgcg
     6721 ttataaccgt aatcagctgc cggagctgat cgagcagatt aacgcttccg gctatggtct
     6781 gacgcttggc gttcatacgc gtattgatga aaccatcgcc caggtcactg gctcagccca
     6841 tgttggtaac ctgtatgtta accgtaatat ggtgggcgca gtggttgggg tgcagccatt
     6901 cggcggcgaa gggttgtccg gtaccgggcc gaaagcaggc ggtccgctct atctctaccg
     6961 tctgctggcg aatcgcccgg aaagtgcgct ggcggtgaca ctcgcgcgtc aggatgcaga
     7021 gtatccggtc gatgcgcagt tgaaagccgc attgactcag ccgctaaatg cactgcggga
     7081 atgggcagca aatcgtccag aattgcaggc gttatgtacg caatatggcg agctggcgca
     7141 ggcaggaaca caacgattgc tgccagggcc gacgggtgag cgcaacacct ggacgctgct
     7201 gccgcgtgag cgcgtattgt gtatcgcgga tgatgaacag gatgcgctga ctcagctcgc
     7261 cgccgtgctg gcggtgggca gccaggtgct gtggccagat gacgcgctgc atcgtcagtt
     7321 agtgaaggca ttgccatcga ctgtcagcga gcgtattcaa ctggcgaaag cggaaaatat
     7381 taccgctcaa ccgtttgatg cggtgatctt ccacggtgat tcggatcagc ttcgcgcatt
     7441 gtgtgaagca gttgccgcgc gggatggcgc aattgtttcg gtgcagggtt ttgcccgtgg
     7501 cgaaagcaat atcctgctgg aacggctgta tatcgagcgt tcgctgagtg tgaataccgc
     7561 tgccgctggc ggtaacgcca gcttaatgac gatcggttaa acaggtagcc ggaggatgtt
     7621 acaccctcct ccggcatcat tagcgtggtc gaatcccctc aataataatc cgctgcacgt
     7681 tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt tgcgcctgtc accgcctcca
     7741 cctgaggggc gaaatcggcg taatgttgag tggaagccca aatcataaaa atcaaatgct
     7801 gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc ggcaatcagc gccgatttct
     7861 catcaattaa tgacttcaaa tcgcccgtca gttcatccat taacagcggc gcgcctgcca
     7921 gcatctccat acagaacagg cgcgaagcct gcggataatc gcgtgagact tccagcttca
     7981 gacggatgta ctctttgatc gccgccagcg gggcgaaatc ttcacgaaac gcttttaacg
     8041 gtgccagcca gatatcgaga atctgccgca gcacggcaat atacagcgcc tcttttgacg
     8101 gaaagtaata cagcagattg gtttttgaaa cacccgccaa ctctgcaatt tgctccagcc
     8161 gtgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc tgcgctaaga atcgcttttt
     8221 tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt cactgcgcct tgcgtcatgc
     8281 gctctcccct cttgtagatt ggcaacagga tagcaaaacc cccgcccggc ctcgaccttc
     8341 tgcactctca tcgcgcacag atgcatgttt tatgtgcaac tgttttgacc gtttagtcca
     8401 ctttttacca gacattttag ataacgcgat gttaacacat taataacact ccctttttaa
     8461 aaactggcat ccgctttgca aacaagccaa tacgcggctg aaaaggatat gcagaatgca
     8521 gcgccccgtc tgactttcac tttacgagac gaagagaggt tgatgatgaa aattggcgta
     8581 ttcgtaccta ttggcaacaa cggctggctc atttcgaccc acgcgccgca gtacatgccg
     8641 acctttgaac tgaataaagc catcgtgcaa aaagcggagc actaccattt cgatttcgcc
     8701 ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg agttctggga tcacaacctt
     8761 gagtcgttca ccttgatggc ggggctggcg gccgtgacct cgcgcattca gatttacgcc
     8821 accgccgcca ccttaacgtt acctccggca atcgtcgccc gtatggccgc aaccatcgac
     8881 tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg gctggcaaaa gcccgagtat
     8941 gagcagatgg gtatctggcc tggcgatgac tatttctccc gtcgttacga ctatctcacc
     9001 gagtatgttc aggtgctgcg cgacctgtgg ggcacgggaa aaagcgattt taaaggcgat
     9061 tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga gtgtccccat gaaagtgatc
     9121 tgcgccgggc aaagcgacgc tggcatggcg ttctccgctc agtatgccga tttcaacttc
     9181 tgtttcggca aaggcgtaaa tacacccacg gctttcgccc cgaccgctgc gcggatgaaa
     9241 caggccgcag agcaaaccgg gcgcgacgtt ggctcttatg tattgtttat ggtgattgcc
     9301 gatgaaaccg acgatgccgc tcgcgccaaa tgggaacact acaaagcggg cgcggatgaa
     9361 gaggcgttaa gctggctaac cgaacaaagt cagaaagata cccgctccgg tactgacacc
     9421 aacgtccgtc agatggccga tcccacttcg gcggtaaaca tcaatatggg gacgttagtc
     9481 ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg caagcgtgcc tggtgccgaa
     9541 ggcgtgctgt taaccttcga cgattttctg tcgggaatcg aaaccttcgg cgagcgcatt
     9601 caaccactga tgcagtgccg cgcccatctc cctgtgctga ctcaggaggt ggcatgatga
     9661 cgacattaac cgctcgaccg gaagccatta ccttcgatcc gcagcaaagt gcgctgatcg
     9721 tggtggatat gcaaaacgcc tatgccacac caggcggcta cttagatctc gccgggtttg
     9781 atgtctcaac cactcgcccg gtcattgcca acattcaaac cgccgtgacc gcagcgcgag
     9841 cggcagggat gttgatcatc tggtttcaaa atggctggga tgaacagtat gtcgaggctg
     9901 gcggccctgg ctcaccgaat tttcataaat cgaacgccct caaaaccatg cgtaaacagc
     9961 cgcagctgca ggggaaattg ctggcgaaag gctcctggga ttatcaactg gtggatgaac
    10021 tggtgccgca gcctggcgat attgtgctgc cgaagccgcg ctacagcggt ttcttcaata
    10081 cgccgctgga cagcattttg cgcagccgcg gaatacgcca tctggttttc accagcatcg
    10141 ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt ttttctggag tatttcggcg
    10201 tggtgctgga agacgcaaca caccaggcgg ggccggaatt tgtacagaaa gccgcgttgt
    10261 tcaatatcga aacctttttt ggctgggtca gcgacgtcga aacgttctgc gacgcgcttt
    10321 ctcccacgtc ctttgctcgt atcgcttaag gagtttaacg atgccaaaat ccgtaattat
    10381 tcccgctggc agcagcgcac cgctggcccc cttcgttccc ggcacgctgg ctgatggcgt
    10441 ggtgtatgtc tccggtacgc tggcttttga tcaacacaat aacgtgctgt ttgccgatga
    10501 cccaaaggcg caaacccgcc acgttctgga aactatccgc aaggtaatcg agacagcggg
    10561 tggcacgatg gcggatgtga ccttcaacag catctttatt accgactgga aaaattacgc
    10621 cgcgattaac gaaacctacg ccgagttttt tccaggtgat aaaccggcgc gattctgcat
    10681 tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc gccacaattg cgcatatcgc
    10741 caagtgaggc cgcgatgaaa ctttcactct cacctccccc ttatgctgat gcgcccgtag
    10801 tggtgttgat ttcgggtctt ggcggtagcg gcagttactg gttaccgcaa ctggcggtgc
    10861 tggagcagga gtatcaggta gtctgttacg accagcgcgg caccggcaat aatcccgaca
    10921 cgctggcaga agattacagt atcacccaga tggcagcgga actgcatcag gcgctggtag
    10981 ccgcagggat tgagcattac gcagtggtcg gccatgcgct cggtgcgctg gtgggaatgc
    11041 agctggcgct ggatcatccc gcgtcggtaa ctgtgctggt ctgcgttaac ggctggctac
    11101 gaataaacgc ccatacgcgc cgctgttttc aggttcgcga acgattactg tatagcggtg
    11161 gcgcgcaggc atgggtggaa gcgcagccgt tgttcctcta tcccgccgac tggatggcgg
    11221 cccgcgcacc tcgccttgag gcagaagacg cgctggcact ggcgcatttt cagggcaaaa
    11281 ataatttact gcgtcgactt aacgccctca aacgcgctga ctttagtcac catgcggatc
    11341 gcatccgctg cccggtgcaa atcatctgcg ccagtgatga tctgctggtg ccatcagcat
    11401 gttccagtga acttcatgcc gccctgcccg atagccagaa aatggtgatg cgctatggcg
    11461 gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc tctgttactc aacgggcttg
    11521 ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat tgctatgaac gaagccgtta
    11581 gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac tcacaacggc tggcgggaga
    11641 cacccgtcag cgatgagacg ttacgggagc tttatgccct gatgaaatgg gggccgacat
    11701 cagctaactg ttctccggca cgtatcgtgt ttatccgcac tgcagaggga aaagaacgtc
    11761 tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct gaccgcgccc gtcaccgcta
    11821 tcgtcgcatg ggacagtgaa ttttatgaac ggttaccgca actgtttcct cacggcgatg
    11881 cccgcagttg gtttacctcc agcccacaac ttgccgaaga aacagcgttt cgcaacagtt
    11941 ccatgcaggc ggcttatctg atcatcgcct gccgtgcgct ggggctggat accggcccga
    12001 tgtcgggttt tgatcgtcaa cacgtagacg acgctttttt tgcaggcagc acgctgaaga
    12061 gcaatctgct gattaatatc ggctatggcg atagcagcaa actttttgcg cgcctgccac
    12121 gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc aaactatgaa cattgtcgat
    12181 caacaaactt ttcgcgatgc gatgtcctgc atgggcgcgg cggtcaatat catcaccacg
    12241 gacggtccag ccgggcgcgc cgggttcacc gccagcgccg tctgcagcgt gaccgacacg
    12301 cctcccacat tactggtgtg cctgaatcgt ggggcgtccg tctggccggt attcaatgaa
    12361 aatcgaacgc tgtgtgtaaa tacacttagc gccgggcagg agccgctttc aaaccttttt
    12421 ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc gctggcagaa tggtgtgacc
    12481 ggatgcccgc aactggaaga ggcgctggtt tcgtttgact gccgtatcag ccaggtggtg
    12541 agcgtcggca cccacgacat tttgttttgc gccatcgaag cgattcatcg tcacgccacc
    12601 ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc tgatgcgccc tgcttgttaa
    12661 cctccttaag gagacagctg atggcaatgt tcggtcttcc tcactggcag ttaaaatcga
    12721 cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt gccgtttgct cagacggcag
    12781 taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac ggtgttaatg ccgattctga
    12841 tggggctgga tcccaatctt tccattttaa tgtcagggat cggcacgtta ctgtttttct
    12901 ttattaccgg cgggcgcgtg cccagttatc tcggctccag cgcggctttt gtcggcgtgg
    12961 tgatcgccgc aacgggcttt aacggtcagg gcattaaccc gaatatcagc attgcgctgg
    13021 gcgggattat cgcctgtggg ctggtttata ccgtcattgg cctggtggtg atgaaaatcg
    13081 gtacgcgctg gattgaacgg ctaatgccgc cggtggtgac gggtgcggtg gtgatggcga
    13141 ttggcctgaa cttagcgcct atcgcggtaa aaagtgtatc ggcttcaggc tttgatagct
    13201 ggatggcggt aatgacggtg ctgtgtattg ggctggtggc ggtgtttacc cgtggcatga
    13261 tccagcggtt attgattctt gtaggattaa ttgtcgcctg tctgctgtat ggcgtgatga
    13321 ccaacgtttt agggctggga aaagccgtcg attttactct cgtcagccat gctgcctggt
    13381 tcggtctgcc acatttttcc actcctgctt ttaatggtca ggcaatgatg ctgattgcgc
    13441 ccgtggcggt gattctggtg gcggaaaact taggtcatct caaggcggtc gccgggatga
    13501 ccggacgcaa tatggacccg tatatggggc gagcattcgt aggcgatggt ctggcaacga
    13561 tgctttctgg ttctgtcggc agtagcggcg tcaccaccta tgctgaaaat atcggtgtga
    13621 tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc tgctgccgtc atcgccatgc
    13681 tgttgggctt ttcaccgaaa tttggcgcgc tgattcatac cattccggcc gcggtgattg
    13741 gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc aggcgcaagg atatgggtac
    13801 aaaaccgtgt cgatttaagc cagaacggta atttgattat ggtcgcagta accttagtgc
    13861 tgggcgcggg tgattttgct ctcacgctgg gcggttttac gttgggagga atcggtacag
    13921 caacctttgg cgcgatttta ctcaatgcgt tattaagcag aaggttggtt gacgttccac
    13981 cgccagaagt tgttcatcag gagccataaa cagaaaaccg ccggggttac cggcggtgat
    14041 ggctttttga cgagcttaag aaaaaaatgc attcgtcatt gattagcccg gctagttgtc
    14101 gcttttgccg tgactgctct tgccaccttt tttacctgct tctgatgctc tttcgcggtc
    14161 ttctgcaaaa ttgccggaac cgcctcgatg gtttgccatt actgacctcc gtctgggtat
    14221 agacatcata ttgctagaat taacgcggcg tatggcggct gccatatttt cgctacgtgg
    14281 gactaagctt agtgaactga cgtgtttcta cccgcgtttg gtaatatcct gcaacagctt
    14341 cttagcgaga atagatatca tctccaataa ttatccataa gccgctctta tgattaaaaa
    14401 aacgcgatga gataaaaatg tgtcattttg cgacaaaatt gcgtgcttgt gaaagttgtt
    14461 ataaatcaaa taagtggttg tgaaatttgc actctgaaaa ggacgtctta tctttaaata
    14521 agtggtagcg aatcgctacg gaatagagat aacacgagga gtggttagaa atggctaaag
    14581 ttctggtgct ttattattcc atgtacggac atattgaaac catgaagcat taaaaacaca
    14641 gaattaccga agatcgtcca agagcatata tttttcaatc tgttacacct cattttgacc
    14701 atgaagaaat caattttaca ccgttcttta tacgttcttt tttgccccaa atatgccc
//
LOCUS       DAZKIF010000115        14152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZKIF010000115 DAZKIF010000000
VERSION     DAZKIF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.116"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            302..601
                     /gene="yoaC"
                     /locus_tag="ACHOTW_003638"
     CDS             302..601
                     /gene="yoaC"
                     /locus_tag="ACHOTW_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP9845677.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(721..900)
                     /gene="yoaH"
                     /locus_tag="ACHOTW_003639"
     CDS             complement(721..900)
                     /gene="yoaH"
                     /locus_tag="ACHOTW_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP9845678.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            974..2335
                     /gene="pabB"
                     /locus_tag="ACHOTW_003640"
     CDS             974..2335
                     /gene="pabB"
                     /locus_tag="ACHOTW_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP9845679.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2339..2917
                     /gene="nudL"
                     /locus_tag="ACHOTW_003641"
     CDS             2339..2917
                     /gene="nudL"
                     /locus_tag="ACHOTW_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP9845680.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3101..4465
                     /gene="sdaA"
                     /locus_tag="ACHOTW_003642"
     CDS             3101..4465
                     /gene="sdaA"
                     /locus_tag="ACHOTW_003642"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9845681.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4596..6194
                     /gene="pdeD"
                     /locus_tag="ACHOTW_003643"
     CDS             4596..6194
                     /gene="pdeD"
                     /locus_tag="ACHOTW_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9845682.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6198..7754)
                     /gene="yoaE"
                     /locus_tag="ACHOTW_003644"
     CDS             complement(6198..7754)
                     /gene="yoaE"
                     /locus_tag="ACHOTW_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP9845683.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7742..7900)
                     /gene="yoaL"
                     /locus_tag="ACHOTW_003645"
     CDS             complement(7742..7900)
                     /gene="yoaL"
                     /locus_tag="ACHOTW_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP9845684.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8217..9188
                     /gene="manX"
                     /locus_tag="ACHOTW_003646"
     CDS             8217..9188
                     /gene="manX"
                     /locus_tag="ACHOTW_003646"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP9845685.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9251..10051
                     /gene="manY"
                     /locus_tag="ACHOTW_003647"
     CDS             9251..10051
                     /gene="manY"
                     /locus_tag="ACHOTW_003647"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP9845686.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10064..10915
                     /gene="manZ"
                     /locus_tag="ACHOTW_003648"
     CDS             10064..10915
                     /gene="manZ"
                     /locus_tag="ACHOTW_003648"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP9845687.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10970..11428
                     /gene="yobD"
                     /locus_tag="ACHOTW_003649"
     CDS             10970..11428
                     /gene="yobD"
                     /locus_tag="ACHOTW_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP9845688.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
     gene            11739..11861
                     /locus_tag="ACHOTW_003650"
     CDS             11739..11861
                     /locus_tag="ACHOTW_003650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845689.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            11858..12424
                     /gene="mntP"
                     /locus_tag="ACHOTW_003651"
     CDS             11858..12424
                     /gene="mntP"
                     /locus_tag="ACHOTW_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP9845690.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12421..13230)
                     /gene="rlmA"
                     /locus_tag="ACHOTW_003652"
     CDS             complement(12421..13230)
                     /gene="rlmA"
                     /locus_tag="ACHOTW_003652"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP9845691.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13396..13605)
                     /gene="cspE"
                     /locus_tag="ACHOTW_003653"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13396..13605)
                     /gene="cspE"
                     /locus_tag="ACHOTW_003653"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9845692.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13618..13761)
                     /gene="yobF"
                     /locus_tag="ACHOTW_003654"
     CDS             complement(13618..13761)
                     /gene="yobF"
                     /locus_tag="ACHOTW_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP9845693.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3529 a         3520 c         3481 g         3622 t
ORIGIN      
        1 tataatttca attttatcta taaaactaac tatataaata aacataacca tgccgttgtg
       61 ttcgcccttt gattagcaac tctggttttc tttcttagct gaacaatctg gttatagcgt
      121 gcggactgac cgttaatttt ctccatctta ctggataaca caccatgagc tgctaccttt
      181 aaaacacctg aatatcaaca ggttaactcg tgccatatcg ttataatccc tttgatatta
      241 gatgcaaatt aaggtcatat atagccttat cgataacatg gttaatttta aggataagaa
      301 tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg tatcagcgcc
      361 aacaccaagt tcgatgaatg aaagcacggc gaagggaatc tttaaatatt taaaagaact
      421 gggagtaccc gcgagtgccg ctgatattac ggcgcgagct gaccaggaag gctggaaccc
      481 tgggttcacg gaaaaaatgg ttggatgggc aaaaaaaatg gagacaggtg aacgctctgt
      541 gattaaaaat cctgagtact tttcaacata tatgcaggaa gaactaaaag cactggtctg
      601 agttaaattt atatcagcat aaatgggtca ggacgctttt aatcacatat aaaaagcgtc
      661 ctttttctca caatcaaata atcatcagct tctataacga aatcgatacg gattagtgtg
      721 ttactcgtct tcatcctcaa aacgcgccac gattcgctcg ccggagtggt tggcacgcag
      781 ctcttctgcc accagcgcga ttgcctggcc gctgctcatc ccttgtgcca tcagttcctg
      841 gatccgctcg acagcttttt gctgctgttc atgggtgagt gaaggtaaac ctgcaaacat
      901 tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca catagccagt
      961 agagtcagga ctgatgaaga cgttatctcc cgctgtgatt actttaccct ggcgtcagga
     1021 cgccgctgaa ttttatttct cccgcttaag ccacctgccg tgggcgatgc ttttacactc
     1081 cggctatgcc gatcatccgt atagccgctt tgatattgtg gtcgccgatc cgatttgcac
     1141 tttaaccact ttaggtaaag aaaccgttgt tagtgaaagc gaaaaacgca caacgaccac
     1201 tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc gcccaacgca
     1261 taacgaagat ttgccatttc agggcggcgc actggggttg tttggctacg atctgggccg
     1321 ccgttttgag tcactgccag aaattgcgga acaagatatc gttctgccgg atatggcagt
     1381 gggtatctac gattgggcgc tcattgtcga ccaccagcgt catacagttt ctttgctgag
     1441 tcataatgat gtcaatgccc gtcgggcctg gctggaaagc cagcaattct caccgcagga
     1501 agatttcacg ctcacttccg actggcaatc caatatgacc cgcgagcagt acggcgaaaa
     1561 atttcgccgg gtacaggaat atctgcacag cggtgattgc tatcaggtga atctcgccca
     1621 gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc ttaatcaggc
     1681 caaccgcgcg ccatttagcg cttttttacg tcttgaacag ggtgcaattt taagcctttc
     1741 gccagagcgg tttattcttt gtgataatag tgaaatccag acccgcccga ttaaaggcac
     1801 gctaccacgc ctgcccgctc ctcaggaaga tagcaaacaa gcagaaaaac tggcgaactc
     1861 agcgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg atatcggtcg
     1921 tgttgccgta gcaggttcgg taaaagtacc agagctgttc gtggtggaac ccttccctgc
     1981 cgtgcatcat ctggtcagca ccataacggc gcaactacca gaacagttac acgccagcga
     2041 tctgctgcgc gcagcttttc ctggtggctc aataaccggg gctccgaaag tacgggctat
     2101 ggaaattatc gacgaactgg aaccgcagcg acgtaatgcc tggtgcggca gcattggcta
     2161 tttgagcttt tgcggcaaca tggataccag cattactatc cgcacgctga ctgccattaa
     2221 cggacaaata tactgctctg cgggaggtgg aattgtcgcc gatagccagg aagaagcgga
     2281 atatcaggaa acttttgata aagttaataa gatattacgc caactggaga agtaagacgt
     2341 ggaataccgt agcctgacgc ttgatgattt tttatcgcgc tttcaacttt tgcgcccgca
     2401 aattaaccgg gaaaccctaa atcatcgtca ggctgccgtg ttaatcccca tcgtccgtcg
     2461 accgcaaccg gggttgttgc tgactcagcg ttcaattcat ctgcgtaaac acgctggaca
     2521 agtggcattc cctggaggtg cagtcgatga cacggacgca tcagttatcg ccgccgcgct
     2581 gcgcgaagct gaagaagagg ttgctatacc gccttccgcc gttgaagtta tcggcgtgct
     2641 gccgcccgtc gatagcgtaa ctggctacca ggtaacccca gtggtcggca ttatcccgcc
     2701 cgatctgccg tatcgcgcca gtgaagatga agtttccgct gtgtttgaaa tgccgctcgc
     2761 ccaggcatta catctgggtc gttatcaccc tttagatatc taccgccgcg gcgattcaca
     2821 tcgggtatgg ctatcctggt acgaacagta ttttgtatgg ggaatgaccg caggcataat
     2881 tcgtgagctg gcgctgcaaa ttggtgtgaa accctgacta tacttatctt tacatctaca
     2941 aaacactact tgagacaatc atcgcaatat tagttaaatc gcggtttttg attagtttaa
     3001 ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta cactatgcgc tgttattagt
     3061 tcgttactgg aagtccagtc accttgtcag gagtattatc gtgattagtc tattcgacat
     3121 gtttaaggtg gggattggtc cctcatcttc ccataccgta gggcctatga aggcgggtaa
     3181 acagttcgtc gatgatctgg tcgaaaaagg cttactggat agcgttactc gcgttgccgt
     3241 ggacgtttat ggttcactgt cgctgacggg taaaggccac cacaccgata tcgccattat
     3301 tatgggtctt gcaggtaacg aacctgccac cgtggatatc gacagtattc ccggttttat
     3361 tcgcgacgta gaagagcgcg aacgtctgct gctggcacag ggacggcatg aagtggattt
     3421 cccgcgcgac aacgggatgc gttttcataa cggcaacctg ccgctgcatg aaaacggtat
     3481 gcaaatccac gcctataacg gcgatgaagt cgtctacagc aaaacttatt attccatcgg
     3541 cggcggtttt atcgtcgatg aagaacactt tggtcaggat actgccaacg aagtgagcgt
     3601 gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa ccggctattc
     3661 gctgtctggt ctcgctatgc agaacgaact ggcgctgcac agcaagaaag agatcgacga
     3721 gtatttcgcg catgtctggc aaaccatgca ggcatgtatc gatcgcggga tgaacaccga
     3781 aggtgtactg ccaggcccgc tgcgcgtgcc acgtcgtgcg tctgccctgc gccggatgct
     3841 ggtttccagc gataaactgt ctaacgatcc gatgaatgtc attgactggg taaacatgtt
     3901 tgcgctggca gttaacgaag aaaacgccgc cggtggtcgt gtggtaactg cgccaaccaa
     3961 cggtgcctgc ggtatcgttc cggcagtgct ggcttactat gaccacttta ttgaatcggt
     4021 cagcccggac atctataccc gttactttat ggcagcgggc gcgattggtg cattgtataa
     4081 aatgaacgcc tctatttccg gtgcggaagt tggttgccag ggcgaagtgg gtgttgcctg
     4141 ttcaatggct gctgcgggtc ttgcagaact gctgggcggt agcccggaac aggtttgcgt
     4201 ggcggcggaa attggcatgg aacacaacct tggtttaacc tgcgacccgg ttgcagggca
     4261 ggttcaggtg ccgtgcattg agcgtaatgc cattgcctct gtgaaggcga ttaacgccgc
     4321 acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg ctggataagg tcatcgaaac
     4381 gatgtacgaa accggtaagg acatgaacgc caaataccgc gaaacctcac gcggtggtct
     4441 ggcaatcaaa gtccagtgtg actaatactt cttactcgcc catctgcaac ggatgggcga
     4501 atttatcccc gctttctcgt ctgctgtaat attccccact acacttccac tgttgcgtca
     4561 ggcgtttgtc gccatacgct tacagagtgg cccgcatgca aaaagcacaa cggatcatta
     4621 aaacctatcg ccgtaatcga atgattgttt gtacgatttg cgcactcgtt acgctcgctt
     4681 cgaccctgag cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg gtagtacaat
     4741 tcgccaatca cgctgtagag gaattagata aagtactgct tcccctacag gcaggtagcg
     4801 aagtcttgct tccgctgatt ggtctgccct gctctgtcgc ccatttgcca ttacgtaaac
     4861 aggcggcaaa actccaaact gtgcgatcca ttggcctggt gcaagacggc acactttatt
     4921 gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga cattctggct gaacttcctg
     4981 caccgcaacc acttttacgc ctgacgaccg accgtgtcct gattaaaggc agtccggttt
     5041 tgattcaatg gacgcctgca gcgagcagta gcaatgctgg ggtcatggag atgattaaca
     5101 tcgacttact gacggcaatg ctgcttgagc cacaactgcc gcaaatcagt agcgccagcc
     5161 tgacggtgga caaacggcat ttgctctatg gtaatgggct ggtagattcc cttccacaac
     5221 ctgaagacaa tgaaaactat caggtttctt cgcaacgctt ttcttttacc attaacgtta
     5281 atggtccggg ggctccggcg ctggcatggc actatcttcc aacacaatta ccgctggcgg
     5341 tactgctaag tttactggtg ggctacatcg cctggctggc gaccgcttac cgaatgagct
     5401 tttcccgcga aatcaatctg ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt
     5461 tgcttaatgc gcgcagccag caatgtattg gtgtagagat tttgctgcgc tggaacaatc
     5521 cgcgtcaggg ctggatttca ccggatgtgt ttattcctat cgcggaagaa catcatttaa
     5581 ttgtgccact gacccgctat gtgatggcag aaaccattcg tcagcgccat gttttcccga
     5641 tgagtagtca gtttcatgtt ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc
     5701 tgataaaaga tctcaatcag tactggttta gcgctcaccc gattcagcaa ctgatcctcg
     5761 aaatcaccga acgcgatgcc ttactggatg ttgattatcg gattgcccgc gagctacatc
     5821 gtaaaaacgt caaactggcg attgatgact tcggcaccgg caacagttca ttttcctggc
     5881 ttgaaacatt acgtcctgac gtgctgaaaa ttgataagtc atttaccgca gctataggtt
     5941 ctgacgcggt taactcgacg gtgaccgata tcatcatcgc gctggggcaa agactgaata
     6001 ttgaactggt ggcggagggc gtggaaacgc aagaacaggc gaagtatttg cgccgtcatg
     6061 gcgtgcatat tttgcaaggg tatttgtacg cacagccgat gccgctacgt gattttccca
     6121 aatggctggc gggcagccaa ccgccgcccg cccggcataa tggacatatc acgcccatta
     6181 tgccgttacg ttaacgctta ctcatcttca tcgtgcgccg gttgctcttt aacaatgcga
     6241 accagatcaa cacgataatc attggcttca atgatggtga tatgcagtgg ccctacatca
     6301 atcacatcgc ccacacgggg aatgtgacca tttgccgaga tcacgaggcc cgcgaccgtc
     6361 gcgatatcat cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa ggcatgcaaa
     6421 tctgtaccgc cttttaccag ccagccgtca ccatcagtaa tgatttccgg cgtttcgtca
     6481 gcgtccggga attcacccgc aatggcttcc agcacatcca gcggcgtgac caagccttgt
     6541 accacaccaa actcgttggt cacgataaca aagctcccgc gagcacgacg cagcacgccc
     6601 agcaggttga tcggatcgag ggtttcaggg acgataatcg ccggagacgc cgaagcaatc
     6661 gccgccacat caacgccctc ttccagcgcc accagcagtt ctttagcacg tacgattccg
     6721 atgatttcat ccagttcacc gcgacatacc gggaacaggc tgtgcggtga ggagagcagc
     6781 tgctcgcgga tttcatcgac cccgagatta gcgtcaaccc agcttatttc accgcgcggc
     6841 gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat catgtaacgt
     6901 tcttcttcgg caaatgcacc ttccgggatc ggcatcggca tcgggttatc ggcatcgtgc
     6961 tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc agtacgcgct
     7021 cgcagcggca aagtcgactg gtgacgaata aagttgcgac gcgcaatctg gttaaacact
     7081 tcgatgatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat gtggaaaccg
     7141 aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca gagcaccacc
     7201 accgtggggt gctggttaac gaatcgcgtc agcggtttgg atgccagcaa cataaccgcc
     7261 atcgcaatca ctaccgccgc catcatcacc ggcagatggt taaccatccc tactgcagta
     7321 attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac gaccacccag
     7381 aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag ccgttcatgc
     7441 agttcggttg ttgctttgaa cagcaagaat atccccccga acaacataat caggtcgcgt
     7501 ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac catccatgaa
     7561 atcagcgaca gcagccccag acgcataatc agcgccagtg ataaccccag caaacgcgct
     7621 ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac caggttatcg
     7681 atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat tgaggggtcc
     7741 attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc agaaataatg
     7801 caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag agtgccagaa aggcaggcgt
     7861 taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct actgccctat
     7921 actccatgat tgttaaacgg gagttaaaca tatcagagac gcctctgatt tggcaaagat
     7981 ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat ttctgctaat
     8041 cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc taaaataaat
     8101 cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat tgtggacgac
     8161 gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg tagcaagtga
     8221 ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt aaaacggcag
     8281 aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca ggtgaaaatg
     8341 ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc actaaaggcg
     8401 tgctgtttct cgttgataca tggggaggca gcccgttcaa tgctgccagc cgcattgtcg
     8461 tcgacaaaga gcattatgaa gtcattgcag gcgttaacat tccaatgctc gtggaaacgt
     8521 taatggctcg tgatgatgac ccaagctttg atgaactggt tgcgctggca gtagaaacag
     8581 gccgtgaagg cgtgaaagca ctgaaagcca aaccggttga aaaagccgcg ccagcacccg
     8641 ctgccgcagc acccaaagcg gctccaactc cggcaaaacc aatggggcca aacgactaca
     8701 tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc gccacccgct
     8761 ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg gctgcggata
     8821 ctgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca cacgtagttg
     8881 atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa cgtgtaatgc
     8941 tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg aaaatcacct
     9001 ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaagtgaat aacgcggttt
     9061 cggttgatga aaaagatatc gaggcgttca aaaaactgaa tgcacgcggt attgagctgg
     9121 aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc agcaaaatcg
     9181 ataagtaacg tattgtgttg attatcagtc agttttcaca ctttagtcat acgtaaacag
     9241 gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg tagcctgtat
     9301 cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa tcgcgtgtac
     9361 cctggtgggt atcgttcttg gggatatgaa aaccggtatt attatcggtg gtacgctgga
     9421 aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg ccgctctggc
     9481 atctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg caggtattgc
     9541 actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc gtactattac
     9601 cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga cagcgatttc
     9661 ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc cggccgtcat
     9721 cgttgcgctg tctgtaggta ccagcgaagt acagaacatg ctgaatgcga ttccggaagt
     9781 ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt atgcgatggt
     9841 tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct tcgtaaccgc
     9901 agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta tggcagtgct
     9961 ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc aggcagctgg
    10021 taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg atacaactca
    10081 aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc tgcgttctaa
    10141 cctcttccag ggttcatgga acttcgaacg tatgcaggca ctgggtttct gcttctctat
    10201 ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac aagctattcg
    10261 ccgtcacctg gagttcttta acacccagcc gttcgtggct gcgccgattc tcggcgtaac
    10321 cctggcgctg gaagaacagc gtgctaatgg cgcagagatc gacgacggtg ctatcaacgg
    10381 tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct tctggggaac
    10441 cgtacgtccg gtatttgcag cactgggtgc cggtatcgcg atgagcggca gcctgttagg
    10501 tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt actacggcgt
    10561 agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg gcttcctgca
    10621 aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat tggttaacaa
    10681 gtggacacat gtcaacatcc cgctggttgt ctctcgcatt actgaccaga cgggcaaaga
    10741 acacgttact actgtccaga ctattctgga ccagttaatg ccaggcctgg taccactgct
    10801 gctgaccttt gcttgtatgt ggctactgcg caaaaaagtt aacccgctgt ggatcatcgt
    10861 tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac tgtaagactg
    10921 ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa tgacaatcac
    10981 ggacctggta ctgattcttt tcatcgccgc actcctggcc ttcgcgatct acgatcagtt
    11041 catcatgccc cgccgtaacg gccccaccct gctggcaatt cctttgctcc ggcgtggtcg
    11101 catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca acgtcacgaa
    11161 tcatggtgcg ttaataacga catggttatt aagcgcactg gctctgatgg gtttttatat
    11221 attctggatc cgcgttccga agatcatctt taaacaaaaa ggttttttct tcgccaatgt
    11281 ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg tgttggtgat
    11341 gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatc tggaaagaat
    11401 ttataaactt ctcgtttcaa ctcaatgagt tattaattta gccaaagcta tgtttagtgt
    11461 atttttaata atcagacata gcttaggcta tattacctct tcccttattt gttatttatt
    11521 ataacgtttc attgatatat aaatctaaat gaaaatcgtt atcaacaaag caataaaata
    11581 atatattcca acagttgttt tatattctca aaatatgtta aggttgcgcc ctcatttggg
    11641 gagtagccaa tttccagatt ccggaaatgt acgtgtcaac atactcgttg caaaacgtgg
    11701 cacgtacgga ctgaagactt tcagtcaggc gagaccatat gcacatcaat cgctatgcct
    11761 gcatgaacgc gaaaacccat cgcggcttgt tgttcatgta tgcatgtttg ctgggggcga
    11821 tgatgtgttt tatggatacc ccggtcagga cgttgtcatg aatatcactg ctactgttct
    11881 tcttgcgttt ggtatgtcga tggatgcatt tgctgcatca atcggtaaag gtgccaccct
    11941 ccataaaccg aaattttctg aagcattgcg aaccggcctt atttttggtg ccgtcgaaac
    12001 cctgacgccg ctgatcggct ggggaatggg catgttagcc agccggtttg tccttgaatg
    12061 gaatcactgg attgcgtttg tgctgctgat attcctcggc gggcgaatga ttattgaggg
    12121 ttttcgtggc gcagatgatg aagatgaaga gccgcgccgt cggcacggtt tctggctact
    12181 ggtaaccacc gcgattgcca ccagcctgga tgccatggct gtgggtgttg gtcttgcttt
    12241 ccttcaggtc aacattatcg cgaccgcatt ggccattggt tgtgcaacct tgattatgtc
    12301 gacattaggg atgatggttg gtcgctttat cggctcaatt attgggaaaa aagcggaaat
    12361 tctcggcggg ctggtgctga tcggcatcgg cgtccagatc ctctggacgc acttccacgg
    12421 ttaataagaa cgctgccaga ggtgaatatt aaagtccgtc tggcagtcga acacttcttt
    12481 tgctgccagt atttgccaga cttctggctt cgcacgccag gcaaacggcg tcatctgcaa
    12541 taatgcgacg gcttcatcac cgcgaagacg catcggataa cacaactcat cactcttctg
    12601 taatgtaaaa ccttccagtt gttctgcatg aggtgcatga agatgtactt cattgtaaat
    12661 cagccccttc agctccatca aatgtcgcgg tcccggcgtg gcagtaatga cccagccgcc
    12721 gggcttcact actcgtgcta attcttctgc tttacacggc gcgtaaatac gtattatggc
    12781 gtccatactg gtatcggaaa acggcaaacg gtggctggaa gcgacacaaa gagtgacctg
    12841 cggatagcgt ttcgccgccg cttttatcgc taccttcgaa acatccagac caaacgtggt
    12901 gatttcgggc aacgcatcgg caaatgcgtg tgtgtaatac ccttcaccac agccaatatc
    12961 cagcaccgcc gtggcctttt cattaagccg ttcccgcagt tgggcgacaa ttgcatcacg
    13021 cagcggctga taatgtccgg catctaagaa tgcgcggcgt gcttgcatca tttccgcgct
    13081 gtcgcccgga tcacgagacc gtttatgctg aacgggcagc agattgacat acccttcttt
    13141 cgccatatca aactgatgtc gttggggaca gatatagctg tttttttcac gcgtaagagg
    13201 ctgatggcaa agtggacagg aaaacgacat gacaactccg gcaggatact aaaggccgca
    13261 agtgtaacgc gaatcacgcg gcgggcaaat atccgaacgc caggagcgaa gataaagcat
    13321 agtaaaaagc ctcgcattcg acgaggcttt atagcgagat tgaagcgtat tcacacttca
    13381 gatcagtgga ttcgatcaga tagctgttac gttaacagct gccggacctt tctggccgtc
    13441 ctgaatttcg aactcaacgt tctgaccttc agccagagtt ttgaagccat taccctggat
    13501 agcggagaag tgtacgaaca cgtctttgct gccatcagca ggagtaatga agccaaaacc
    13561 tttagactcg ttgaaccact taacctgacc tttaatcttt gccatttgaa aaattcctta
    13621 gattgttttc ttcgcccgca ggcataacat agataaaact gacacattac tgcatgaggc
    13681 accaatataa ggctcggcag agaagcggta ttcaacgtca acgtgtttac tcaggacttc
    13741 tttactgaaa atgccacaca taaacagaac tgtacctcgt ttaacccgaa atctgttatc
    13801 acatacaacg ttaattatgg caagccattt ttaaacatgt ctcgatcaga cgcacaaatc
    13861 tcgatacgtc ttccactttt tttgcacact tatgcaacgg aatacgcgcc gatatatcat
    13921 tgtgcttaac cttgtcagtt ccgcagatac ttaaactgac gtagtttcta acatagttca
    13981 ataagttcag ttgttccagg tcagtgggag aagttattac atagtgcgtg caatatcaca
    14041 ttttctgcta tgcaatgaat aaaaagttat atcacttttt ctcataaaac aggcagttaa
    14101 cggctctcaa tcaccccaaa gagaagtcaa tcccaaagga attggaaatt ta
//
LOCUS       DAZKIF010000116        14105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZKIF010000116 DAZKIF010000000
VERSION     DAZKIF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.117"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..2234
                     /locus_tag="ACHOTW_003655"
                     /pseudo
     CDS             1..2234
                     /locus_tag="ACHOTW_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860777.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2244..5873
                     /gene="yehI"
                     /locus_tag="ACHOTW_003656"
     CDS             2244..5873
                     /gene="yehI"
                     /locus_tag="ACHOTW_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFP9845694.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            5935..6252
                     /gene="yehK"
                     /locus_tag="ACHOTW_003657"
     CDS             5935..6252
                     /gene="yehK"
                     /locus_tag="ACHOTW_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFP9845695.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            7493..8581
                     /gene="yehL"
                     /locus_tag="ACHOTW_003658"
     CDS             7493..8581
                     /gene="yehL"
                     /locus_tag="ACHOTW_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP9845696.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8592..10871
                     /gene="yehM"
                     /locus_tag="ACHOTW_003659"
                     /pseudo
     CDS             8592..10871
                     /gene="yehM"
                     /locus_tag="ACHOTW_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            10864..12000
                     /gene="yehP"
                     /locus_tag="ACHOTW_003660"
     CDS             10864..12000
                     /gene="yehP"
                     /locus_tag="ACHOTW_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9845697.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            11997..13997
                     /gene="yehQ"
                     /locus_tag="ACHOTW_003661"
                     /pseudo
     CDS             11997..13997
                     /gene="yehQ"
                     /locus_tag="ACHOTW_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
BASE COUNT         3491 a         3637 c         3629 g         3348 t
ORIGIN      
        1 ttatgcagat agcagcaagg acagttatag aaggaataac gacaaattta gtttacgtct
       61 gcgtaaacat ttatcactag cagaagaaga agtatggcaa aattgcattg caaaattact
      121 cgttgcgtta ccagatatat cactctatcg ccaaccgtta attgccattt taatgccaga
      181 aatacccgaa gtggcccatg agatcgtaca tcgtttacac caggtcgcag atgttccaca
      241 acttgaaatg ttgaaactgg tagcgacaga tccattcacg ctcgaaatac tggagaacta
      301 tcaatacatt gatgtattta acacctatgg tgccagttgg tcagctacgg tattacggga
      361 gcaaggtata gcggcactgg ctcgcctgac tccctatgct gaagaagata attgcggtga
      421 cgtactgaaa tgtattaatc atccgttggc cattacgaat aaaaagtgcc gaaaattttc
      481 cttctgcaac gatagtggca ttagccgaag cattggtaaa aaaagatgat aaacgcaaac
      541 atacattgct ggtttcatta cttaatgcaa atcctttatt tgtcgaaaaa ttagtcatcg
      601 gattatcgtc gcaagcggca aatttattgc aacaatgtca gcaattgctg aatagtcgca
      661 atgaagattt tgcaaactca gaaattctac caatggtact agtctcgccg ccctgggaaa
      721 ctcaaaataa aaataacgac aacagtcctg tgggtaaata cccggcgaaa atcccccctt
      781 taccgttgtt ttatcaacct ccattatgga ctcgcccgaa actcaaaaat agcagcaagg
      841 tactgccaga tagcgcactc cagcacctcg gtgaaatgct ccgcttccct caggaagagg
      901 ccctgtatcc gggattattg caggtgaaag atgcctgtac cgccgactca ctggctgaat
      961 ttgcctggga tctgtttacc gcctggctga ctgcgggcgc accgtcgaaa gagagttggg
     1021 cgttcactgc gttaggcgtt cttggtaacg atgacaccgc ccgcaaactg acgccattaa
     1081 tacgcgcctg gcctggtgaa tcccagcata aacgcgccac cgttgggttg gatattctcg
     1141 ccgctatcgg tagtgatatc gccctgatgc agcttaacgg tattgcccag aaactgaaat
     1201 tcaaagcatt acaggagcgg gcaaaagaaa aaattgccgg cattgccgaa agccgcgaac
     1261 tcacggtggc agagcttgaa gatcggttag caccggatct cggtctggat gataacggtt
     1321 cgttgctgct ggatttcggc tcacgccagt tcaccgtcag ctttgatgaa accttaaaac
     1381 cgtttgtgcg tgatgtttct ggcagccgtc tgaaagacct gcccaaacca aacaaaagcg
     1441 atgatgaatc acaggcgaac gatgctgtta accgctacaa actgctgaaa aaagatgcgc
     1501 gtaccgtcgc cgcccagcag gtagcaaggt tggaatccgc catgtgcctg cgtcgccgct
     1561 ggtcgccaga aaactttcag ctcttcctgg ttgaacatcc actggtacgc cacttaaccc
     1621 gccgtctgat ttggggcgtt tatagcaccg aaaaccagct actgacgtgc ttccgcgtgg
     1681 cggaagataa cagctacagc accgctgacg atgatctttt caccctgccg gaaggcgata
     1741 tctctgttgg cattcctcac gttctggaaa tatcaccgac ggatgctacc gcctttggtc
     1801 agctttttgc cgactatgaa ctgctaccac cgttccgtca gcttgaccgt aacagttacg
     1861 ccctgacaga agccgagcgc aacgccagcg aactgacccg ctgggcaggc agaaaatgcc
     1921 cgagcggtcg ggtcatgggg ctggcgaata aaggctggat aaagggcacc ccgcaggatg
     1981 gaggctggat cggctggatg atcaaacctc tgggccgctg gtcgttaatc atggaaatcg
     2041 atgaaggttt tgcggtaggc atgtcgccag ccgaactgag tgccgagcaa atcttaagca
     2101 agctgtggct atgggaaggc aacgcagaaa gctatggctg ggggagtaat tcaacacagg
     2161 aagcgcagtt ctccgtactc gatgccatca ccgccagcga gctaattaac gatattgaag
     2221 ccctgtttga ataaggaaag agcatggaca aggaattacc gtggctggcg gataacgccc
     2281 aactggaact gaaatataaa aaaggcaaaa cgccgctcag tcatcgtcgc tggccgggcg
     2341 aaccagtgtc cgttatcact ggaagtctca tccagacatt gggagatgaa ttactacaac
     2401 aagcaggcca gaaggaaaat atcacctgga attatgataa atgctcactg gagtggcagt
     2461 ccgccatcca gcaggcaatc aatttgactg gcgagcataa accgtcaatc ccagccctta
     2521 ccatggcggc tctgatctgt atcgcgcaaa atgacagcca gcagttactt gatgaaatcg
     2581 tgcaacaaga ggggctggaa tatgcgacgg atgtagtgat atcgcgtcag tgcattgcgc
     2641 ggcgttatga gagtgactca ctggtcgtca ctttgcagta tcaggacgag gactatggct
     2701 acggttatgg ctccgcgacc tataacgatt tcgatctccg tttgcgtaag catctgtcgc
     2761 tggcagagga aagttgctgg cagcgttgtg ccgataaact cattgccgca ctgccaggaa
     2821 tacccaaaat tcgccgtcct tttattgcgc tgatcctccc ggaaaaacct gaaatagcca
     2881 atgaactcgc cagtcttgaa agctcccggt caagtctcca ttcaaaggag tggttaaagg
     2941 tcgttgctac tgataatacg gcggtaaaaa aactcgaacg atactggggc ctggacgtat
     3001 ttagcgatcg cgaagccagc tacatgtcac aagaaaaccg cttcggctac gcggcctgcg
     3061 cctccctttt gcgcgaacaa ggactggcag ccgttccgcg cctggcaatg tatgctcata
     3121 aagaagattg cggcagtctg ctggtacaaa ttaaccatcc gcaagtcatc cgcaccttgc
     3181 tgttagttgc tgataaaaac aaacccagcc tgcaacgtgt ggctaaatac agtaaaaact
     3241 tcccccatgc gacgctcgcc gcactggcag aactgctggc gttaaaagaa ccaccagccc
     3301 gccctggtta tccaatcatc gaagacaaaa agctgcctgc acagcaaaaa gctcgagaag
     3361 aatactggcg tacgctgtta caaacgctga tggcatcgca gccacaactg gcagcagaag
     3421 tgatgtcgtg gttaagtact caagccaggg cagtgctgaa gagttattta tcggcactgc
     3481 caaaaccggt tattgatggc accgataaca gcaatctgcc tgaaatcctc gtttcaccac
     3541 cgtggcgtag taagaaaaaa atgacagctc cacgtcttga tttggcacgg ctcgaattaa
     3601 ctccgcaagt ttactggcaa ccaggcgaac gagagaggct tgccgccact gagtctgccc
     3661 gttatttcag cacggaatct cttgcgcaac gcatggaaca aaaaagtggg cgagttgtat
     3721 tacaggaact gggttttggg gatgatgtat ggctgttttt gaattatata ctccctggaa
     3781 aactggatgc tgcacgcaat tcactcattg ttcagtggca ttactaccag gggcgggttg
     3841 aagagatcct gaatggctgg aactccccgg aagcacaatt agcagaacag gcgctccgca
     3901 gcggtcacat agaagcgtta attaacatat gggaaaatga caactactca cgttatcgtc
     3961 cggaaaagag tgtctggaac ctgtatttat tggcacagtt gccgcgtgag atggctttga
     4021 ctttctggct gcgtatcaat gagaaaaagc atctgttcgc gggagaggac tattttctca
     4081 gtatcctcgg attggatgcg ctaccaggtc tgctgttggc tttttcacat cgtccaaaag
     4141 aaacatttcc gttaatttta aatttcggcg caacagaact ggccctgccc gttgcccgcg
     4201 tctggcgacg tttcgcggcg cagcgtgatc tggctcgcca gtggatttta caatggccgg
     4261 aacatacggc tactgcactt atccctcttg tcttcaccaa atctagcgat aaaagcgaag
     4321 ctgcattact tgccctgcgt ttactttacg aacatgggca tggcgaattg ctacaaactg
     4381 tagccaatcg ttggcagcgt acagatgtat ggcctgccct ggagcattta ctgaaacagg
     4441 gtcccatgga aatttacccg gcacgcattc caaaagcccc tgatttctgg catccgcaaa
     4501 tgtggtccag gccgcgcctt atcactaata atcaacctgt taccgatgac gctctggaaa
     4561 ttatcggcga aatgctgcgc tttacccagg ggggacgttt ttatagcggg ctggaacaac
     4621 tgaaaacgtt ctgccagcca caaacgctgg cagcttttgc ctgggatctc ttcactgcgt
     4681 ggcaacaagc tggtgccccc gcaaaagaca actgggcatt tctggcgtta agtctctttg
     4741 gtgacgaaag cacggcacgg gatctaacaa cacagatcct cgcctggcca caaggcaaat
     4801 ctgcccgtgc tgtcagcggc ctgaacatcc ttaccctgat gaataatgat atggcgctga
     4861 tacagctgca tcatatatcg caacgggcta aatcccgccc cttacgtgat aacgcggcgg
     4921 aatttcttca ggtagtcgca gaaaatcgcg ggctaagcca ggaagagcta gcggacagat
     4981 tagtcccaac cctgggcctt gatgatccgc aggcgttgag ttttgatttt ggtccccggc
     5041 agtttaccgt tcgcttcgat gaaaacctca acccggttat ctttgatcag caaaacgttc
     5101 gccagaaaag cgttccccgg ttgcgcgccg atgacgatca actgaaagcg cccgaggcac
     5161 tggcccgact aaaagggcta aaaaaagatg ctactcaggt gagcaaaaac ctgctcccgc
     5221 gtcttgaagc tgccctacgt accacccgac gctggtcgct ggcagatttt cattctctgt
     5281 ttgttaatca tccctttacc cgtctggtta cccagcgatt aatatggggg gtttatccgg
     5341 caaatgaacc gcgtcgttta ctcaacgcct ttcgtgtggc cgcagagggg gggttctgca
     5401 atgcgcaaga tgagccaatt gacctgcctg cggacgctct gattggcatt gcccacccgt
     5461 tagaaatgac agcagaaatg cgcagtgaat ttgcacagct ttttgccgat tacgaaatta
     5521 tgccgccttt tcgccagttg gcgcgccgca cggtgctgct cacacctgat gagtcaacca
     5581 gtaacagcct gactcgctgg gaaggtaaat ccgctaccgt tgggcaactt atgggaatgc
     5641 gatacaaagg ctgggagtca ggttatgagg acgcatttgt ctatgacctg ggtgagtacc
     5701 ggctggtcct taagttttca cccggtttta accactacaa tgttgatagc aaagcgttaa
     5761 tgagcttccg ttctcttcga gtgtaccgtg acaataaatc cgtcactttt gccgaacttg
     5821 atgtgtttaa tttgagtgag gcgttaagcg cacctgacgt cattttccat taaaaacaca
     5881 gcgggcagtg atgcaactgc ccgttatcaa cacgacttac cagcggggaa agcgatgatc
     5941 gtgcaaaaag agctggttgc tatttacgat tatgaggtcc ctgtacctga agatccgttt
     6001 tccttcagac ttgagatcca taaatgctct gaattattta caggttccgt ctatcgactg
     6061 gagcgattcc ggctacgttc aacatttcat caacgtgatc gagaagatgc tgacccgcta
     6121 ataaatgatg cgttgattta tataagagat gagtgtattg atgagcggaa attacgaggt
     6181 gaatcacctg aaactgtaat agcaattttt aatcgtgaac tacagaatat attcaaccaa
     6241 gaaatagaat aatatactct agataattaa agtcgcagca catgcaactt gaagtatgac
     6301 gagtatagct ttcttaaagg gggaaacggt attccccctt agatgcgttt caatttgccc
     6361 gttacttcaa caaatcaatc aaccgtaaca gagccactaa taactccagt acggcaatga
     6421 gcacggttaa cttttgtgtc agcgtgtcca tccgctctcc ttattaagga gcgcaacacc
     6481 agcaccttac ccttacactg cctgtcgata ctggtttacg tatgttggta cgaaacgttg
     6541 ctctccggat cagggcaccg cctgcggcac cacccgtccg gcagccagga cttaaaattg
     6601 gtccgatccg gcacaacctc ccaacgggca ggattataaa tctcactcac tgaattgcaa
     6661 tggagtgtgg atatggttat aatttattcg ttggtacgaa atgttgcaca atcagcaagt
     6721 tagcgtcact gtagtaaata aaagaacctg cctcaccagc aggttttttt attttctgcg
     6781 atctactttc cagatatttt tttgccaata gcccaccata aatattcaca tcccggtcaa
     6841 atggtgcgtt gaatataaaa tcgatgaatt tataaataaa aatgagttat atgatgaatt
     6901 aataaaaaca gtaatcattt tattaaaggg ggaagcggtt ttccccctta gatgcgtttc
     6961 aatttgcccg ttacttcaac aaatcaatca accgtaacag agctactaat agctccagta
     7021 cagcaatgag cacggttaac ttttgtgtca gcgagtccat ccgctctcct tattaaggag
     7081 cgcaacacca gcaccttacc cttacactgc ctgtcgatac tggtttacgt atgttggacc
     7141 acgatgttgc actccggatc agggcaccac ctgcagcacc ccccgtccgg tagccaggac
     7201 ttgaaatagg ttcgatccgg cgcaacgtcc caacagtcag gattataaat ctcattatct
     7261 taattgcaac ggggtccagc cgtggttata attcgttcgt tggaacacga tgttgcacag
     7321 gctgtggtgt taggcctgaa aatagtaaat aaaagaacct gcctcaccag caggtttttt
     7381 tatttactgt gatctgcttc ccagatattt ttcgctcaaa caactaatgc gccaaacatt
     7441 tattgcgcgt aaaatatcgt ttatttcatt aatacatttc agggatgaat atatgtcacc
     7501 gcagaataat catctgcagc gtccgcctgc tgctgtgtta tacgccgatg aactggcaaa
     7561 attaaaacaa aatgataacg caccttgccc gcccggttgg cagttaagtt tgcctgcggc
     7621 ccgtgctttt atccttggcg atagcgcgca aaatattagc cgtaaagtgg tgattagtcc
     7681 ctccgctgtc gaacgtatgt tagtcactct tgccaccgga cgcggtttga tgttggtggg
     7741 ggaacctggc accgcaaaat ctctcctgtc tgaattactg gcaacctcta ttagcggcga
     7801 tgccgggtta accatccagg gcggcgcatc tactaccgaa gatcaaatca agtacggctg
     7861 gaactacgcc ctgcttatca atcatggtcc atcaacggaa gcgctggtcc ctgccccgct
     7921 ttaccaggga atgcgcgacg gcaaaatcgt ccgttttgaa gagataacgc gtacaccgct
     7981 ggaagtgcaa gactgtctgt taggtatgct ttctgacagg gtgatgacgg tgccggaact
     8041 cactggtgaa gccagtcagc tctatgcgcg agaaggcttt aatattattg ccactgccaa
     8101 tacccgcgat cgcggcgtca atgaaatgag tgctgcgcta aagcgtcgct tcgattttga
     8161 aactgtcttt ccgattatgg atttcgccca ggagctggag cttgtcgcca gtgcttcggc
     8221 gcgtttgctg gcccatagcg gtattccgca taaagtgcct gacgcggtac tggagttact
     8281 ggtccgcacc ttccgcgatc tgcgtgccaa cggcgaaaag aaaacgtcaa tggatacact
     8341 aacagcgatt atgtccaccg ctgaagccgt gaacgtggcc cacgctgtgg gcgtccgcgc
     8401 ctggttttta gcgaatcgcg cgggggagcc tgccgacctg gtggactgta ttgccggaac
     8461 catcgtcaaa gataacgaag aagatcgcgc tcgtctgcgc cgttacttcg aacagcgtgt
     8521 cgccacacat aaagaagctc actggcaggc ttattatcaa gcccgccacc gcctgccgtg
     8581 aggaaagatg catgagcgag ccgttaattg tcggcatccg gcatcatagt ccggcctgcg
     8641 cccgactggt gaaatcgtta atcgaaagcc agcgaccacg atacgtgttg attgaaggcc
     8701 cggctgattt taatgaccgg gtagatgaac tgtttttagc ccaccagctt ccggtggcta
     8761 tttacagtta ttgccagtat caggacggtg ctgctcctgg gcgcggcgcc tggacgccat
     8821 ttgccgaatt ttcgccggag tggcaggcgc tacaagccgc acgtcatatt caggcacaaa
     8881 cttactttat cgatttgcct tgctgggcgc agagtgaaga agaggacgat tcgcctgata
     8941 cgcaagagga aagccaggcc ttgctgctgc gtgccacccg catggataac agcgataccc
     9001 tgtgggatca cttgttcgaa gatgaaagcc agcaaactgc attaccctct gcactggcgc
     9061 actattttgc ccaactgcgg ggcgatttcc ccggcgatgc actcaatcgt cagcgcgaag
     9121 cctttatggc tcgctggatt gcatgggcag tgcagcaaaa taatggcgac gtgttagtcg
     9181 tctgcggtgg ctggcacgct ccggcactgg caaaaatgtg gcgcgaatgc ccgcaggaca
     9241 ttaacaagcc agaattgccc tcgctggcag atgccgttac aggttgttat ctcacaccct
     9301 acagtgaaaa gcgccttgat gtgctggcag gatacctttc aggaatgcct gccccggtct
     9361 ggcaaagctg gtgctggcag tggggcttac agcaggcggg agaacaactg ctaaaaacgg
     9421 ttcttacccg tttgcgccaa caccacttgc ctgcttcgac cgcggatatg gctgccgctc
     9481 atctgcatgc gatggcgctg gcacagttgc gcggatataa actaccgtta cgcactgact
     9541 ggctggatgc catagcaggc tcgctgatta aagaagccct gaacgcgccg ttgccgtgga
     9601 gctatcgcgg cgttattcat cccgataccg atccgattct gctaacgttg atagacacat
     9661 tagcgggtga cggattcggt aaacttgccc cttctacgcc acaaccgcct ctgccaaaag
     9721 atgtcacctg cgaactggaa cgtaccgcaa tctctctttc ggcggagctt accttaaatc
     9781 gctttaaccc caatggacta gcgcaaagtc aggtgttaca tcggctggca atactggaga
     9841 tcccagggat tgtacgccag cagggaagca cactgacact tgcaggcaac ggtgaagaac
     9901 gctggaaatt aacccgcccg cttagccagc atgcggcatt gattgaggcc gcctgctttg
     9961 gtgccacact ccaggaagcc gcacgccata aattagaagc cgatatgctg gacgcgggtg
    10021 gaatcggcag tatcaccaca tgtcttagcc aggcggcgtt agcgggtctg gcgtccttca
    10081 gtcaacaatt actggagcaa ctcacattat taatcgccta ggaaaatcaa tttgccgaaa
    10141 tgggcccggc gctggaagtg ctatatgcct tatggcggct ggatgaaatt agcggtatgc
    10201 aaggtgcgca gatatcacaa acgacgttat gcgcggctat cgatcgcacg ctgtggctgt
    10261 gtgaatctaa cggcagaccg gatgaaaagg agtttcacgc tcacctgcat agctggcaag
    10321 cgctttgcca tattctgcgc gatctacata gcggcgttaa tttacccggc gtttctcttt
    10381 ctgcggcggt agccttactg gagcgacgca gtcaggcaat ccatgccccg gcgctggatc
    10441 gcggcgcggc tcttggcgca ctgatgcgtc tggaacatcc caacgctagt gccgaagcgg
    10501 cgctgacgat gctggcgcag ttatccccgg cacaatccgg cgaagcgctg cacggtttgc
    10561 tggcgctggc ccgccatcaa ctggcctgtc agccggcatt tatcgccggt ttcagcagtc
    10621 atttaaatca accaagtgat gccgatttta tcaatgccct gcccgattta cacgcggcga
    10681 tggcctggct accgccacga gaacgcggga cactggcgca tcaggtgctt gaacattatc
    10741 aactggcgca acttcccgtt tcggcgctgc aaatgccgtt gcattgccca ccacaagcca
    10801 ttgcacatca tcaacaactc gaacagcagg cactggcatc gctgcaaaac tggggagttt
    10861 tccatgtctg aactgaacga tcttctgacc acccgtgagc tacaacgctg gcgattaatt
    10921 cttggcgaag cggcagaaac gacgctttgt gggctggatg acaacgcccg gcagatagac
    10981 cacgcgctgg agtggctgta tgggcgcgat cctgaacggc tccagcgtgg tgaacgctcc
    11041 ggtggattag gtggctcaaa tctcaccacc cctgagtgga tcaacagtat tcacacgctg
    11101 tttccgcaac aggtgattga gcggctggaa agcgatgccg tactgcgcta cggcattgaa
    11161 gatgtggtga cgaatctcga cgtgctggaa cgtatgcagc cttctgaaag cctgctacgc
    11221 gccgttttgc acaccaaaca tctgatgaac cccgaagtac tggctgccgc ccgccggata
    11281 gtgcgccagg ttgttgaaga aattatggct cgactggcaa aggaagttcg tcaggctttt
    11341 tctggtgtcc gcgatcgccg tcgccgctca tctattccac tggcgcgaga ctttgatttc
    11401 aaaagtactc ttcgcgccaa tctgcaacac tggcacccgc aacacggcaa gttgtatatc
    11461 gaatcccccc gctttaacag ccgaattaag cgccacagtg aacaatggca actggtctta
    11521 ctggttgatc taagcggatc gatggtcgat tcggtgatcc actctgcggt aatggcggcc
    11581 tgtttgtggc agttacccgg cattcgtacc catctggtgg cgtttgacac cagtgtcgtt
    11641 gatctcacgg cagacgttgc cgatccggta gagttattaa tgaaagtaca gttgggcggc
    11701 gggaccaata tcgccagtgc cgtggagtat gggcggcaac ttattgaaca accagcaaaa
    11761 agcgtcatta tcctcgtgag tgatttttat gaagggggtt catcatcatt gctgacgcat
    11821 caggtgaaaa agtgtgtcca gagcggcatc aaagtgctgg ggctggcagc gctcgacagc
    11881 accgcaacgc cttgctatga ccgcgatatg gcccaggcgc tggttaatgt tggcgcacaa
    11941 atagccgcaa tgacaccggg tgaactggct acctggcttg cggagaattt gcagtcatga
    12001 attcactacg tccggaatta ttagaactca cgccgcaggc cctgacggcg ttaagcaatg
    12061 ccggttttgt taagcgcagc cttaaggaac tggaaaatgg caacgtcccg gaaatcagcc
    12121 atgagaacgg cgctttaatc gccaccttca gtgacggtgt ccgtacccag ctggcgaacg
    12181 gccaggcact gaaagaggct cagtgcacct gcggggctag cggcatgtgc cgtcatcgcg
    12241 tgatgctggt gttaagttat cagcgacttt gtgccaccac ccggcccacg gaaaaagaag
    12301 aagagtggca gcctgcaatc tggctggaag aactggctac cctgcccgat gccacccgca
    12361 aacgtgcaca ggtgctggtc gctaaaggca tcaccattga gttgttctgt gcgcctggcg
    12421 aaattccctc agcccgctta ccgatgagtg atgtgcgttt ttattcccgc agcagcattc
    12481 gcttcgcccg ctgtgattgt attgaaggca cactttgcga acatgtcgta ctggctgtgc
    12541 aggccttcgt cgaggccaaa actcagcaag cggaatttac tcatttaatc tggcagatgc
    12601 gcagcgaaca cgtcacatca tctgacgacc cgtttgccag cgaagaaggc aacgcgtgtc
    12661 gtcaatatgt tcagcaatta agccaggcat tatggcttgg cggcatcagc cagccgctta
    12721 ttcactacga ggccgctttc agccgcgcgc agcaagcggc ggaacgctgc aactggcgct
    12781 gggtgagtga atcgctacgg caactgcgcg ccagcgttga tgccttccac gcccgcgcca
    12841 gccactatca tgcaggagaa tgcttacgtc agcttgcggc attaaacagt cgattaaatt
    12901 gcgcacaaga gatggcccgg cgcgacagtg ttggtgaagt tcctcctgtg ccgtggcgca
    12961 cggtcgttgg ctctggcatt gccggagaag caaagcttga tcatctgcgg ctggtgtctt
    13021 taggtatgcg ttgctggcag gatattgagc attatggttt acgcatctgg tttaccgatc
    13081 ccgacaccgg cagtattttg cacctttcgc gcagttggcc gcgaagtgaa caggaaaact
    13141 caccggcagc tacgcgtcgg ctgtttagtt ttcaggctgg cgcactggcg ggtgggcaaa
    13201 ttgtttcaca agcagcaaaa cgcagtgccg atggcgagct gctgttagct acccgcaacc
    13261 gcttaagcag cgttgtgccg ctgtcgcctg atgcctggcg aatgttgagc gcgccgttac
    13321 gccagccggg cattgtggct ttgcgggaat atttacgcca gcgtcccccc tcctgcatac
    13381 ggcctcttaa tcaggtcgat aacttattta ttctgccggt cgctgagtgt atttcgctcg
    13441 gttgggatag cagccgccag acgctggatg cgcaggtaat cagcggcgaa ggtgaagata
    13501 atctgctgac gttatcactg ccagcctcag ccagcgcgcc ttatgccgtt gaacgcatgg
    13561 cggcgctttt gcaacaaaca gacgaccccg tgtgtctggt ttctggcttt gtcagttttg
    13621 ttgatgggca attgacactg gaaccacagg taatgatgac aaagactcgt gcctgggcgc
    13681 tggacgcaga aactgcgcct gtggtggcat cactaccttc tgccagcgtt ctgcctgtgc
    13741 cgtctactgc tcatcagttg ctgatgcgct gccaggcatt acttattcaa ctgctccata
    13801 acggctggcg ctatcaggaa cagagtgcta ttggtcaggc agagttgctg gcgaatgacc
    13861 tcaccgcggt gggtttttat cggctggcac atgtattggg acaatttcgt aatacagaaa
    13921 gcgaggcacg ggtagaagca atgaataacg gtgttttgct gtgcgaacaa ttattccccc
    13981 tgcttcagca acaaggattg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac
    14041 tcaggctgcc agatcatcgt ttccgatgga agcataataa gctttttctg cttctgccgg
    14101 aggag
//
LOCUS       DAZKIF010000117        13658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZKIF010000117 DAZKIF010000000
VERSION     DAZKIF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.118"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            123..1181
                     /locus_tag="ACHOTW_003662"
     CDS             123..1181
                     /locus_tag="ACHOTW_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFP9845698.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1191..2333
                     /locus_tag="ACHOTW_003663"
     CDS             1191..2333
                     /locus_tag="ACHOTW_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFP9845699.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2326..3099
                     /locus_tag="ACHOTW_003664"
     CDS             2326..3099
                     /locus_tag="ACHOTW_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845700.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3101..4180
                     /locus_tag="ACHOTW_003665"
     CDS             3101..4180
                     /locus_tag="ACHOTW_003665"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFP9845701.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4180..5136
                     /locus_tag="ACHOTW_003666"
     CDS             4180..5136
                     /locus_tag="ACHOTW_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFP9845702.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5147..6355
                     /locus_tag="ACHOTW_003667"
     CDS             5147..6355
                     /locus_tag="ACHOTW_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFP9845703.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6373..6840
                     /gene="terW"
                     /locus_tag="ACHOTW_003668"
     CDS             6373..6840
                     /gene="terW"
                     /locus_tag="ACHOTW_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFP9845704.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7101..7430
                     /locus_tag="ACHOTW_003669"
     CDS             7101..7430
                     /locus_tag="ACHOTW_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9845705.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7417..7758
                     /gene="tnpB"
                     /locus_tag="ACHOTW_003670"
     CDS             7417..7758
                     /gene="tnpB"
                     /locus_tag="ACHOTW_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9845706.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            7840..8670
                     /locus_tag="ACHOTW_003671"
                     /pseudo
     CDS             7840..8670
                     /locus_tag="ACHOTW_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8701..10314)
                     /locus_tag="ACHOTW_003672"
     CDS             complement(8701..10314)
                     /locus_tag="ACHOTW_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFP9845707.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10345..10695)
                     /gene="tnpB"
                     /locus_tag="ACHOTW_003673"
     CDS             complement(10345..10695)
                     /gene="tnpB"
                     /locus_tag="ACHOTW_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9845708.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10692..11117)
                     /locus_tag="ACHOTW_003674"
     CDS             complement(10692..11117)
                     /locus_tag="ACHOTW_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9845709.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11171..11922
                     /locus_tag="ACHOTW_003675"
                     /pseudo
     CDS             11171..11922
                     /locus_tag="ACHOTW_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            13455..>13658
                     /locus_tag="ACHOTW_003676"
     CDS             13455..>13658
                     /locus_tag="ACHOTW_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845710.1"
                     /translation="MEFSVIFFWVITGLITILFLKSIITDLIKNLNKTDIALYILAFL
                     SAGYTFQYIYNDNIFSELWGVALF"
BASE COUNT         3310 a         3576 c         3533 g         3239 t
ORIGIN      
        1 acactgacgc actccataac cacaggagtt attaatcata ttatgattga catgtagctt
       61 agacactcat agactcactt ctcaatcata aatttgcatg gcgttccgga taattaaaaa
      121 aaatgaacaa gaaaatttgg tttatggaag gtttatcctc ccagcgagat attattcagg
      181 gggtaaaatc atttgcacaa aaaaataatt ttgccattac cgtttttgcc tcccaccgta
      241 acgaaagaaa tgaaatcctt tccgttgccg attattcttt gactgaacct gaagatcctc
      301 aaaaacgtct tcagtttatc caggaaacca ttcagaccta cggcatccac catattcata
      361 ctggccgtaa cagccagtgg tttgaagaac accgttcagc cattgaaccg accggtgcca
      421 cccttactac cggtgcaacg ggcgtcgact ggttaactct ggctgacgaa aaagttactt
      481 ttgctcagtt tatggagcaa aagggtctcc cggtcgtacc atcctggcgg gtgaatacgc
      541 tggcagaatt aaagacacac ctcgcggccc cgccgttcac tgacagcccg gtatgcgtga
      601 agccggtgac gggtatctat ggcatgggat tctggcgctt tgatgacagt gcttcgccta
      661 tggccgtctt taatcatccc gaacatcgtc tggtcagtcc gcaacagtat attgcagcag
      721 catcagctgc tgagtcgttt aaaccccttg ttttgatgcc gtacctgcca ggcccggaat
      781 tttccgtcga tatcctcgcg gataagggcg aaatactcgc agccgtggga cgccgtaagg
      841 aaggggctat ccagtatctg gtaaacgaag gaagcgcctg ggaactggcg tgtgactgcg
      901 cccgtgttat gaaggccgac gggctggtga atgttcagac gcgaaacgat gtgaatggca
      961 acccggtgct gcttgaaacc aacatgcgtc cgtcaggggg ggtgggttat acccttcaca
     1021 gcggtgtgaa ccttcctggg ttatttgctg cctttaagct cggtctgatg tctgaagata
     1081 tggtacgcca gagcgctaaa aacacctttt ctccggttgc ggtgagatcc attacggatg
     1141 taattgcata cccggaatca ctctctaacc ttctgaatta aggcgtattt atgtctgata
     1201 tcctcgaaga tatgatttat cgccgtaccc tctcatgcgg tacgattcag gtaacccgtg
     1261 accagggtga agtttcgctc gatgacctgt ttgacatcgc tgaaagacgt aacccgaaac
     1321 gcgcctttct gtttgtcagc aaagtactcg gcaggcacat tcccgtgcca ccctcagtca
     1381 tgcggcaggc ctacagacag cttgccagcc agttcccctc gacactgaca ggacccgtac
     1441 tgtttatcgg tatggctgaa accgccgttg ggcttggggc cggtgtattt gatgaagtgc
     1501 gccaccagca tcgtgaatct gtctatctga cctctacccg tcacccggtt gatggcacgt
     1561 tgctttgcga gttcaaggaa gaacacagcc acgccaccga tcatctgatc tatctgccag
     1621 atgatgaaga gaaaagacgt cgtgttacca acgcacgaac gctggttttg attgatgacg
     1681 aggcaaccac cggtaatacc tttattaacc tgctttctgc cctgcgtaat accggcaagc
     1741 ttcaacatat tgaacaggtt atcgccgtaa cgctgaccga ctggagtggc aatgccctgt
     1801 cggagcgcac ccccttgccc gtcacttcgg tttcgcttgt aagcggtcag tggggatgga
     1861 ccccattacc tgatgcacct gtcccggaca tgccgaaagt caacgtaact tcacgaggag
     1921 aatgggacat ccaggggaaa cagtcatggg gccgactggg gatgctcgca cctgcggccg
     1981 atctcggcca tgaggtctcc gtccacaagg gggaacgtat tctggttctc gggaccgggg
     2041 aattcgtctg ggagccgttc ctgcttgctg aacggctcga agctgccgga gcacaggcat
     2101 tttatggatc gaccacccgc tcccctgtcg ccgttggtta tgccattgag tccgccattt
     2161 cctttacgga taactacggg ctgggcatcc ccaattttgt ctataacgtc gcccaccagc
     2221 agtttgaccg cattcttgtg tgtactgaga cacccgcaga aagtattgac acgcagcttc
     2281 ttaaagcgct ggctgaggtt gcgcccgtcg tggagattgt tacctatgaa taaacccgtt
     2341 gttctgagcg accttgacga cacgctcttt cagacccgtc gtaaaatggt ggacgagctg
     2401 gccctggagc cattccgtac cggtgccgtt gatcgcaccc tgaatccacg aagctttatg
     2461 acggaagaac agtccatgtt ggtggactgg ctcctggagc aggctgaact catacccgtt
     2521 accgcacggg gaactgaaga aatcagccgc gtacggatcc ctttccactc ctgggcaatc
     2581 acaacgcacg gggccgtcat tctcacgcct gaaggcaaac ccgacgagga gtggaaggcc
     2641 catatgctcg gccagctggc tccctaccag gaaaagctga catcgatgca gcgtctgatc
     2701 actgaaatga tggacgcaaa aggaatcaat gcctgggcaa ggctgaactt cgaatacggt
     2761 gaaacggcgg tttatatggt gatgaaacac cgcgacagca cccgccttga cgagctcaat
     2821 gccattgcag atgagataga aacggtgttt ccgaccgagg gcttctacat ccaccgcaac
     2881 agtaataacg tggcctggct tcccaccccg gttgagaaag ggctggcagt cagctggctg
     2941 cttgaaaaac ttcgggctga acgaggagtt ttccccgtaa ttggtttggg cgacagcctg
     3001 agcgatcatc gttttatgaa actgtgcagc tggtttggca tcccgcgtca gagtcagttt
     3061 gcagatgcca tttcacagcg aatttttgga gaaaattaaa atgcagccac atgaaacatt
     3121 taccggctca taccagcccg gtgacgtgga atttctgcta aagccggttg tcattgagat
     3181 gacgccggtt gagaaaaaag aagaactgat tcagtccggg aagaaacatt attcggacat
     3241 gctcagccag gagccagcgc ctacacaatg gcatcttgat ttgtttcacc gggcgctgga
     3301 tcggggcgca gagaggcttg caaaagaagt cacacagctc gctattgctc tggccgaacg
     3361 cttcggtgat gagcccattg tactggccag tcttgtcagg gctggcgttc cgctcggcgt
     3421 tatgctgcac caggccctgc gtgacatggg gaaaacctca tggcattacg gtatcagcat
     3481 tatccgggat cgtggaattg acggtgcagc cctcgacgtc attgaagagc ggcatggtac
     3541 cagcggtatt gtctttgttg acggatggac aggtaaaggc gcaattaccg gagagcttgt
     3601 acgcgcactg aaagatcgcc cgggctatcc tgagcagccg cgactggttg tcctggccga
     3661 tccctgtggc tgctcctggc ttgcagccag tgatgatgac tggctcattc cttttggcat
     3721 catgggggcg ccggtatcag gcctgatctc ccggtcagta tggtcctctg aaggattaca
     3781 cgggtgcatg gtttgcgagc atctcagtga attcgaatgc agccggatgc ttgccgatac
     3841 cgtcgctcat ttccgtaaga agttaacacc gtcatccctc gctcccctga gctggaatac
     3901 ggaatcagcc cgggtcttat ggcagacaag tcgcgacgtt atcgcgttcc tggccgatga
     3961 atttaaagtg gacagcgtca atcgtattaa acccggtatt gctgaagcaa cccgggctgt
     4021 attacgtcgg gtaccggacc atgtatttgt gcgttctatt gacgacccgg acgttgcctt
     4081 gcttgtaggg cttgctcgtg aaaagggaat agttgttaca gaaatggggg gaaccctcgg
     4141 ccagtatcgg gctgtaacca ttatcaagaa ggtactctga tgaaaaatcg tctttctccc
     4201 tggaatctgg gagccacgct atacatgcct gcaacacggg aagatattgc tgatgccgtc
     4261 ctgcacggga aaatcccggg cttgcgctct ctggtgattt gcctggagga tgctgtcagt
     4321 gaagcagaca tccccgtcgc cctcaaaaat ctggagcacc tgctgcacga gctcagtaac
     4381 agcatgagta gcctgggtaa aaatgactgg cctctggtgt tcattcgccc ccggcatgcc
     4441 gaaatgggca gatggttaaa agcgcattat gatctttccg ctgtcgacgg gtttgtgctg
     4501 cctaaattta ccctcagttc gttagctgaa tggtgggata tcatggtcgg gactcacctg
     4561 tgcatgatgc caacgctgga aacagaagat gtctttgacg tggttcagat gcgcgagctg
     4621 gctacccgtc tggaggaaca tccctgccac gaccgcatta ttgcgcttcg aatcggcggc
     4681 aacgatctta tgaacgttgt gtcgcttcgc cgtccccggg acctgacgct gtatgacagc
     4741 ccgatgggct acgtcatcaa aatgctcgtt tcggtcttcg gcccgcgtga ttttgccctg
     4801 accgcgcctg tatgtgagca tattgacgat catgccgtta tggccagaga gctggctctt
     4861 gatatggcac acgggcttgt tgggaaaacg gccattcacc cgggtcagat agaggtcatt
     4921 caaaacgcgc tgatggtcac tcagggtgag cattctgacg ctctgcggat cctgaactct
     4981 acccaggccg tgtttaagtc gcagggagca atgtgtgaac ccgccacaca tcgccgctgg
     5041 gcggctggca ttctggacag ggctcgtttt tatgggttac agaacgagca aagcgctgat
     5101 ggaatcagat tacttaccgt gacccagcat cattaaggaa atcataatgg ggttcaggtt
     5161 cagaaagtcg atcaacatta ttcctggcgt tcgcctcaac ctgagtaacg gtgcaccgag
     5221 cctgagtgtc gggccgagag gtgcttccgt ttcttttggt agccggggga cctatgccaa
     5281 tctgggcttg cccggtaccg ggctgagtta ccgtacccgg cttgaccggg ccgcgcgttc
     5341 cagaggtgaa aaccggacgg caaccgaccc ggggctcaga caggcgcttg agcagaaagc
     5401 cgctgaactc atgtcagcgg taaccgcaat ccgtaatatt cacgagctga cgccggatcc
     5461 aaaaacaggc atcagctggg cagagctgga agcagtatac ctgcataaca gaacgtcgcc
     5521 ttttcaggtt ccggcaccgg tgcgtccaga aaagccagac taccttgtat tgccggaaaa
     5581 gcctgccgag agcgaaggca ttagtttttt gggtaaatgg tttgaatcgg aatcagctaa
     5641 agctgagcgc cacgccgaaa atcttcgccg gtggcagcag gagctgattg atgtggagcg
     5701 tgagaatacc cttcgacagc accggtacca gcaacaacgg acggcctggg ccgaacagta
     5761 tgcaaactgg aagtttgaag ctgaagaaca tgaaaaacgg ctcgccacgg ctcaggcaga
     5821 tgcccggcag cagttccgga cagacgccgc gtttttcgaa tcatacctgg cgggtgtgct
     5881 ggcagaaact gaatggccgc gtgaaacgct tgttgcattt gaagtaaagc cggagctatc
     5941 agcagtcctg ctggacgttg atttagctga gattgaagat ttccctgata agatttacgg
     6001 cgttaatgcc cggggaacgg agctgacgga aaaagccatg acgcaaaaag ccgtacgcga
     6061 aaactatgcc caccacgtcc atggctgctt gttccgcctg gtcggtatcg ttttacatac
     6121 gctacctttc gacaacgtga ttgtgtcagg ctttacgcaa cgggtcagta agcggaccgg
     6181 ctatctggag gatgagtata tcctgtcctg caaatgcact cgcagccaga tgtcgtcagt
     6241 aaattttgca ggcataaaac acattgatcc ggttgaagcg ttaggcgatc aaccggttat
     6301 tcgaaagatg agcagtacct tcatttttca gcctattgaa ccactaaccc tttaagcagg
     6361 ctaaatctta cgatgcaatt aaataccaga caggcccgga tttttaagct tgccaactta
     6421 ctgggcaccg gcaagcccgt ttccgctgcc gacattataa ccagtctgga atgctctgag
     6481 ccgacattaa cccgggccct caaagagcta cgcgagtcat attctgcgga gatcaaatac
     6541 agcaaggcag gtcattccta tcatctcgtt aatccgggcc agttggataa aaagaccctc
     6601 cgccggatga atgaagcgct cgcacagaat gctgaactga aaaccggtga gtccacaggg
     6661 aaggtcgtac tcgataagga taaaaaaaca gccgtgtcat tatcgctacg catgcggatt
     6721 ctaagaaaaa ttgaccgtct ggctgcgctg agtgactcga cccgaagtga agcggtagag
     6781 aagctggctc tgcactccgt tgatgaactg ataaaagaat acagcgctaa aaagtcctga
     6841 cgctaaatct cagaccgggg tgaaaactcc ggttttgtca tatttcacct ctgtattggc
     6901 gtgatgcaaa atggcatctg aatcgatcat ggtttggcat ccatttccga tcactgtttg
     6961 gcatcacaat cgatcacgat ttggcatgct tccgctcatt gattggcatc ctggtgtcgt
     7021 tacggggaag tcatcttcac agggatcttc agtcagttag cattaccgac atccattcac
     7081 caacttaccg gagataatca atgtcaaaac cccgatggac gcttgatcag aaaaaacatc
     7141 acgttgccgc atggcgcgcc agcggcctta cccgtgaaca gtactgtgaa ctttacgaca
     7201 ttccgtttaa atccctgcgc cagtggccgc aggatgtcgc aaaagccgaa aaacgagcca
     7261 gagcaccaga aattattcct gtttcagtat ctggctcttc cggaatgact gatggcagac
     7321 cattgtcgga tgagcctgtc actctcttcc tgcccggtgg tatccggatg tgctgccagc
     7381 cttctcagtt aaccgatgtt ttccgggctc ttcgccatgc tgacgcctga taacgtattc
     7441 ctggtcgtaa aacccgtcga tatgcgccgg ggtatcgaca cactgacaca gtacgtccag
     7501 aacgaactta acgctgcatg gcatgacggt gctgcttttg tcttcaccaa taaagtccgc
     7561 tcccgcataa aagttctccg gtgggacaaa catggcgtat ggctgtgtac ccgacgactg
     7621 caccggggaa gcttccgctg gccccggaaa ggtgatgcca catggcatct cacacaggat
     7681 gagtttcact ggctggtctt tggggttgac tggcaacagg tgaaagggca tgacctggca
     7741 aaatgggtat accagtaaga acccgaagat gctcagagca acgcaataac tcaatgaaat
     7801 ataatgaata tccatgcgtg atgtcatata atccaccgca tggatatctc gcttctcagt
     7861 accaccagtg accctgaaca gcttcgcgct ctggcaattg ccatggtgca aaaagtcatg
     7921 gctgagaatg cagaacttca gaaccgtatc cgcatcctgg aagagcagat gaagctcgca
     7981 cgccagcagc gtttcggtaa aaaatgcgaa tcgctggccg ggatgcagcg ttccctgttc
     8041 gaagaggatg ttgacgcgga tatcgcagaa atctcggcgc atctggacaa actgcttcct
     8101 cagaccgggg acgaagaaaa aacaacaaca cgtcctgttc gtaagccctt gccttcacct
     8161 cttccccgtg ctgaaaaggt tatcccgcca gctgaggaac gttgtcctga ctgtgatgcc
     8221 cccctgcact tcattcgcga tgaagtcagc gagaagctgg aatatattcc ggcacaggtc
     8281 gtggtcaacc gctatatccg cccgcagtac agctgtccct gctgtgagaa agtgttcagc
     8341 ggaaaaatgc cggcacatat ccttccgaaa agtgctgttg agccatcggt catcgcacag
     8401 gttgtcatca gcaaatatac cgaccatctg cccctgtatc gccagcagca cattttctcc
     8461 cgaatggggg tggagctccc cgtcagtaca atggcggata tggtcggtgt tgctggcgct
     8521 gcactggccc cgctggcaaa attgttacgc cacgagcttc tgacacggga tgttattcat
     8581 gcagatgaaa cgtccctgag gctccttgat acccggaaag gcggtaaatc ctgctccggc
     8641 tggctgtggg cttacgtcag cggagaaaga gtaagcgtca acggagcacc gtattgacgc
     8701 ttatttattg gtgagtacta cgttccatgg caggagttca tcaactcggt tggaaggcca
     8761 ttctggcagt acgctcagga tatggcgcag atacgcttcc ggatcgatac cgttcagacg
     8821 gcaggtgccg atcagcccgt acaacagtgc tccacgctcg ccgccgtgat cgctgccaaa
     8881 gaacataaag tttttctttc cgagacagac tgcacgaagc gctctttccg cagcattatt
     8941 atccgcctcc gccagaccgt catcactgta ataacagagg gcatcccact gattcagtac
     9001 atagctgcac gcttcgccca gtctggattt tttcgacagc gtaccattct tctccaccat
     9061 ccattcatgc agtgacgtca gtaacacttt gcttcgctgc tgcctgacgg caagacgctc
     9121 tgactccggt aatccccgta tttcatcctc gatggcgtac agttcactga ttcgcttcag
     9181 ggcttcttct gccgtcgcac ttttgctgct gatgtataca tcgtggattt ttcgccgggc
     9241 atgggcccag cacgcaactt ctgtcagtgc accaccttca cgttctgcac tgaacagccg
     9301 atcgtaaccg ctgaacgcat ccgcctgcag gataccccgg aagggacgaa ggtgttgtac
     9361 cggatgtttt ccctgcctgt ctggtgagta ggcgaaccag accgccggtg gctctgatga
     9421 gcccgcattc cggtcatccc ggacatacgt ccagatgcgt cctgtttttg ccttttttct
     9481 gcccggtgcc agcactttta ctggtgtgtc gtcagtgtga accttgcggg tgttcatcac
     9541 gtaacggtac agggtatcat tcagcagagt cattaactga caacatgcgt caacccagtt
     9601 ggagagtaat gcccggctca gttcgatgcc ctggcgggca aagatttcac tctgacgata
     9661 cagtggcagg tgttcgcagt attttcccgt taacacgcgg gcaagtaatc cggagcccgc
     9721 gatgccgcgc tctatcgggc gggacggcgc tggcgcttca actatacagt cacattttgt
     9781 acaggctttt tttacccgaa cagtgcggat cactttcagg gcgctactca ccagttccag
     9841 ctgctcagca ctgacttccc ccagataatc cagctcactg ccacactccg ggcaacaact
     9901 ttcttcaggc tccagacggt gtatttcacg gggaagatgt gccggtaacg gacgacgatg
     9961 acgtgactgt cgcaactggc ggggaacctg cgggtcatcc tcacgcccac tgtaacgatc
    10021 gctttcctgt tcgcgttgtt tcagttgggc ctcagcctgt tcaacctcac gctgcagttt
    10081 ttcagaacgg gtaccgaaca acatccggcg cagtttttct atctgggccc tcagatgttc
    10141 tatttcccgc tcctcctctt cgatcttttc ctcggcacgt gccagtgcag agcgcaggaa
    10201 ggcctccgtc tcttcaacca gactcagttg ctgatctttc tgacggaggg cttcagcctg
    10261 ctcagagagc agcctttcca gctcagtgat acgaatgagg tatttccgac tcatgaccgt
    10321 ttttataatc cggccatgac atttttacaa cattgtcagt gcattaaggc gggatgtttt
    10381 gggctgacgc cagtccagtt tatcgaggag cattgccagc tgcgagcggg taatggatac
    10441 cttaccgtca cgcaccgcag gccagataaa ctggccttcc tccagacgtt tggtgaacag
    10501 gcacagacca tcagcatcag cccacagaat tttaatcgtg tcaccccgtc ggccgcgaaa
    10561 gataaacagg tgaccggaga aggggttctc atccagcaca tgttgtacct gttctcccag
    10621 accgttgaag gatttacgca tatcagtaac gccggcaacc agccagattc gagtgcctga
    10681 tgggagcgag atcatcgtcc cctcccggtc agttcacgga tcaacaccgt gagcagctct
    10741 ggtgaaggat tttccagcgt catgttaccg tggcggaact caactttaca ggaactggca
    10801 ctgactgtgc tttgtgaagg agtggataaa agcggagtaa gagccgccat aggctctttc
    10861 tgctcatcag gcgttatctc aacaggtaat aattcaacgc cagcgccaga agaggttgtt
    10921 accggaagac gccgcgatat acgcctttcg ttctgccaga gcctgagcca tttgaacagg
    10981 aggttatcat tgatatcgtg ttcccgggca atacgggcaa cagaggctcc tggttgtgaa
    11041 gccagtttaa ccatttgaag tttaaactca tttgaaaatg atctgcgggg ttctgaggat
    11101 aatattttct gttccataac aggtgtccac tagttgaaaa ggtgggcacc tacgttacca
    11161 atactggctt aatggctaca tacggcggtc agtttacgct tacgagaaag aagcggtccg
    11221 ccggtcgtct gttttgactc gcagaccgga cgggccctca gatatcctga ggcctggctg
    11281 cagggctggt gtggcggtac gctggtgagt gacggataca gcgtgtacaa atctctggcg
    11341 gataatgacc ccggtattac atctgcctgt tgctggagcc acgcaaggcg cggtttcgca
    11401 aatctgtata aggccagcag ggagcgccgt gcagccatgg cgctgagaaa aatcgcgggt
    11461 ctttatcgca ttgagaagtt catccgtgaa cgtcctgtgg aaaaaatacg gcaatggcga
    11521 caacggtatt cccggccgat agtgaatgac ctgtttgcct ggcctgaaga gcaggagccg
    11581 tgctgtccgc ccgacggacc actaaacaaa gcgattaatt atatcctgaa ccggcgcgat
    11641 gaactgagct gcttcctgag tgacggagcc gtgcccctgg acaacaatat ctgcgagcgt
    11701 gctatccgcc cggtggtcat ggggcggaaa gcatggttat tcgccggttc actgatggcc
    11761 gggaaccgcg cggcacagat aatgagcctt ctggaaacgg caaaacgtaa tggtctggag
    11821 ccgcatgcct ggctgacaga cgttctgacg cgtctaccgg agtggccgga agaccgactg
    11881 gaagagttgc tgccccttga gggatttacc ttctccgggt gagtgatacc tgccatcagg
    11941 tgttcgtgca gcgggccata acccgcagtc gggagttgaa cgcctgacga caggaaatga
    12001 gccagagcag cgaccacgcg ggctgacagt gagtcagaac ctgaatcagc ccggagccat
    12061 tcccggggct gtacttcatc acctgccatc agtacccgtg tcagtacttc gcgacctgac
    12121 gcaattaccc gactcagttc ttcagaaccc gcctccgata ccagcctggc ggtcgcccct
    12181 gtgcaacaaa ataaatcaac catcgtcaga cggtgatcaa tcgtatgcgc tgccagtggc
    12241 tcgcctgaaa aatgcgtcaa tatgagttcg ccggaacaat acctcagttc ttcagaaccc
    12301 gcctccgata ccagcctggc ggtcgccccg gcgcaacaga ataaatcaac catcgtcaga
    12361 ctgcgatcat tcgtatgcat cgctgatgat gcgcccgaaa aacgcgtcaa tatgagttcg
    12421 ccggaatatt actgacgaca cggtttgtag ttacgttcct tcggtgagtg agtacctgtg
    12481 cgtcagggta acgcaatcag ttcaggttta gcggggtgtc aagtcggttt ggctaaccga
    12541 ccgcagaatt aactgtacct ttaaaggtcg cccccgatat atctgcctgt cagacatatc
    12601 gccgattcgg tatctcacta cataaaactc cttaaaaaac aatgcgattc tgttggtacg
    12661 aagctttcag taagtcagtc cggagaagcg ccagtacgat acagaatgag gttgaaaaaa
    12721 acgttcacgt ctggtcactt cttcaaatgt taaaaaacat ctcgtaaaga gccagtttat
    12781 gcgggttcgt tgagtagacg tgcgagcgtc actttgtcag ctttaacgct gacgaaagtg
    12841 acgcccgcac agcttcaaac tccacatgca cacgcaagcg accaaatttc aaatggtctt
    12901 atgccgtgcg cacagtcata agaagcgcat gcggaaagtc agaaaatatc ttaagacgca
    12961 gattttaaag gcgtttttat gccagtctgc cgggaattca cagtagtccg gttgtccgga
    13021 agcaccacgg taatccgaag gaaaaatcaa cgtacctcaa tcaccgtagt gatatccgtt
    13081 cccagaggct aaacggatgt accaccataa aggcatcctc tttcaggcta cgaggatatt
    13141 gcaatcaccc gaaggcgttt ctctcagatt gctgaaacat tggctggcag tcacccgaag
    13201 gcgtccctct cagactgctg gaacattggc tggtcgcaaa agcgacagtt aatgcgaata
    13261 caggttaaca aaaagggaaa tctgccgtca acccaaccat atgaacatgt tgatttcatt
    13321 atgaatgtga tgttctaata taaattaatc agtaacttca taaaaagaat ataagaaaca
    13381 gataatgcat gtatttacac acatatcagc acaatgatag attaccaccc cagaaacacg
    13441 ataaaggtat aattatggaa ttttccgtta tttttttctg ggttatcact ggtcttatta
    13501 ccatcttatt cttaaaatca ataataaccg atctaataaa aaacctaaac aaaacagaca
    13561 tagcgttata tatattggca tttttatcag ccggatatac attccagtat atttacaatg
    13621 acaatatatt ttcagaacta tggggagttg ccctgttt
//
LOCUS       DAZKIF010000118        13640 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZKIF010000118 DAZKIF010000000
VERSION     DAZKIF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.119"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            223..1053
                     /locus_tag="ACHOTW_003677"
     CDS             223..1053
                     /locus_tag="ACHOTW_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP9845711.1"
                     /translation="MSMPLSNVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1194..1967)
                     /gene="uxuR"
                     /locus_tag="ACHOTW_003678"
     CDS             complement(1194..1967)
                     /gene="uxuR"
                     /locus_tag="ACHOTW_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP9845712.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2182..3642)
                     /locus_tag="ACHOTW_003679"
     CDS             complement(2182..3642)
                     /locus_tag="ACHOTW_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP9845713.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3723..4907)
                     /gene="uxuA"
                     /locus_tag="ACHOTW_003680"
     CDS             complement(3723..4907)
                     /gene="uxuA"
                     /locus_tag="ACHOTW_003680"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9845714.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            5247..6590
                     /gene="gntP"
                     /locus_tag="ACHOTW_003681"
     CDS             5247..6590
                     /gene="gntP"
                     /locus_tag="ACHOTW_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFP9845715.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6764..7666)
                     /gene="fimH"
                     /locus_tag="ACHOTW_003682"
     CDS             complement(6764..7666)
                     /gene="fimH"
                     /locus_tag="ACHOTW_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9845716.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTKNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7686..8189)
                     /gene="fimG"
                     /locus_tag="ACHOTW_003683"
     CDS             complement(7686..8189)
                     /gene="fimG"
                     /locus_tag="ACHOTW_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFP9845717.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8202..8732)
                     /gene="fimF"
                     /locus_tag="ACHOTW_003684"
     CDS             complement(8202..8732)
                     /gene="fimF"
                     /locus_tag="ACHOTW_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP9845718.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8742..11378)
                     /gene="fimD"
                     /locus_tag="ACHOTW_003685"
     CDS             complement(8742..11378)
                     /gene="fimD"
                     /locus_tag="ACHOTW_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFP9845719.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11445..12170)
                     /gene="fimC"
                     /locus_tag="ACHOTW_003686"
     CDS             complement(11445..12170)
                     /gene="fimC"
                     /locus_tag="ACHOTW_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9845720.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12207..12746)
                     /locus_tag="ACHOTW_003687"
     CDS             complement(12207..12746)
                     /locus_tag="ACHOTW_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9845721.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     HRVTGGIANAQAWFSLTYQ"
     gene            complement(12811..13359)
                     /gene="fimA"
                     /locus_tag="ACHOTW_003688"
     CDS             complement(12811..13359)
                     /gene="fimA"
                     /locus_tag="ACHOTW_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9845722.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3327 a         3490 c         3279 g         3544 t
ORIGIN      
        1 aattccaatt agtaataaga tatgatttta aattgcatgc aaataatttt aaatgagcgt
       61 tttttgatac tttagagtgt ttcctaccct ttaatatcac tgtgtagagt tgatatcact
      121 acaacagaaa catgcatgtc atttgtttac ttctcttttt actacagaaa aatttatcta
      181 tttttctaaa catcagtgaa atactcataa aaggtaacta ttatgtctat gccattaagc
      241 aatgtccttc agagtcaaat tattacagat aatcacttcc ttcatcatcc aaaagtcgaa
      301 agtgagttaa cacgtaaata tgaacgtgca cgcctggata cggaaaatat ctatcttctg
      361 cctcttgctc gtggcaataa ccataattac gatggaaaaa gcgttgtcga aattagaaaa
      421 cttgatatta gtaaagaatc ctggccattt aattatgtga cagaagcatg cagagaatcc
      481 gatggtatta caacaacagg acgtatgctc tatcgaaact tgaaaatcac ctcagcttta
      541 gacgaaatat atggtggtat atgtaaaaag gcgcatgctg ctacagagtt agcagaaggt
      601 ttacggctaa atttatttat gaagagccct ttcgatcctg ttgaggacta tacagttcat
      661 gaaataacat taggccctgg ttgcaatgtt ccaggttacg ccggaaccac gataggatat
      721 atttcaaccc ttccagcttc tcaggcaaaa agatggacca atgagcagcc aagaattgat
      781 atctatattg atcagataat aactgtctca ggggtagcaa attcttccgg attcgccctt
      841 gccgcattac ttaatgctaa tattgagttg ggtaatgacc caataattgg tatagaagcc
      901 tatccaggta ccgcggagat ccatgcaaaa atgggatata aagttatccc cggtgatgaa
      961 gatgcgccac tcaaacgcat gactctgcaa ccgtcatctc ttccagaact gtttgaatta
     1021 aaaaatggtg aatggaatta tattggcaaa taaccactga gaagaatgag tactaacatt
     1081 caggttatat agatatttaa acaataccct gcaatttaca aagtaatact cattgcgact
     1141 tcttaattta agaaagcgca aggaacgttt acccttgcgc ttattataat aagtcaggcg
     1201 tcgactttat ccagcggcca tgaatcaaac agatagccgt caaaataaat atcgtcaacg
     1261 ttcgagaatt ccagcagacg ttgcttaacg ttttccagat gctgccacat tgccagcttc
     1321 gccgctcggg catctttttt gattaacgcg gcgaggatct gtttgtgatc gcccaaccac
     1381 tctttgcgat acaggctgtc atccagatgg ctgtgcaact ggatccacat tgggttgttt
     1441 tcccgccact gccaggactg acggaacagc tccaccagca tgctgttatg cgttgcttcg
     1501 gcaatagcga gatggaactg catgtcaccg ctttcgctgc tgcccggcgc actggaagcc
     1561 agttcacgct cttccagttg caatgcctga cgcattttga cgatatcttc gcgggtagcc
     1621 tgcagggcgg caaactcggc gatgttactt tccagtaact gacgcgcctg taacagctca
     1681 aaaggacccg catcattgca gacgttggca tccggactgt ctgtgttgtg gctgcctgag
     1741 ctatcaagaa catagatacc ggcaccccgg cgcacttcca ccagcccttt gatctccagc
     1801 atgatcagcg cttcacgtac caccgtccgc gtgacatcaa gcatttctgc aatttcacgc
     1861 tccggcggta gccgttcacc aggattgtac ggcgtcttta tgatcagatc gcggatcatc
     1921 gccccgactt cctggtaagg tctttgcgca gaggtggcag atttcatggt gttgtgtccg
     1981 ttaggtgcgg ttaatcaaat ttttgccgga tgcggcgtga acgccttatc cggcctacaa
     2041 cgggcaattt tacggccccc gttaatctca aactatacca ttagcactgg aagttggcac
     2101 gtacaggtgg gcaaaaaaac gaaaactggt caaccaaata tcagtttgat tgaccagtaa
     2161 gtctgtaggg taatcagtta gttacagcgc agccacacac tcgcgcgcac cgcgttcgca
     2221 cagctgctga tatgccgcag tcaccgcgcc aacaaagtcg gcattctgcg gcagatcatc
     2281 ggcaaaaata ccgctcaggc ccagcagcgc tttcacgcgg tctgcgccct gatactgcgc
     2341 gttaatcttc tggaactcgg ccagcatcgg gtcgaccacg tcaatggcat taccctgctc
     2401 atccacgccc tgggtgtaac gcatccagcc agccacgccc agcgccaggt gacgccagct
     2461 gccgccgttt tgcaggtgca gacgcaccgg gtccagcaga cgctgcggta acttctggct
     2521 gccgtccatc gcaatctgcc aggtacggtg acgcagagac gggttgctga aacgctcgat
     2581 cagcagcgtc gcataggcgt tcaggtccgt accttccggc atcgacagcg ttggcgcttg
     2641 ttcctgcatc atcagggcaa aggccgcttt gcgataatcc gggttagtca tggtgtcggc
     2701 aatagtttca tagccgccga ggtaaccgag gtacgccagg aaagagtggc tgccgttcag
     2761 catacgcagt ttcatcattt cgaacggcac aacgtctgca acgaactgtg cgcccacttt
     2821 atcccagtcc ggacgaccat taacgaagtt atcttcaata acccactgac ggaacggttc
     2881 gcaggcaatg gcgcacgggt cgtaaacacc cagctggtca gcaatttcct gtaaggtttc
     2941 tggcgtcgcc gccggaacga tgcggtcaac catggtgcac gggaaggtga cgttctcttc
     3001 aatccatgcc gctagctgcg ggtcacgcgc ctgagccagc cccagtaccg cgaccttcgc
     3061 cacatgaccg ttttcacgca cgttatcgca ggacatcacc gtaaatgctt tcagcccttt
     3121 ttcacgacgc agacgcaagg cttcgacgat gtaaccaatc gcggacttcg gcgcagtcgg
     3181 gttttccaga tcgtgcttga tcagcgggtt attgagatcc agttgaccgc ttgccgcatc
     3241 agcgcagtag cctttttccg tgaccgtcag ggagacaatc gccgtttgcg gacgcgccat
     3301 cgcgttgaga ataccttcgc agccgtcgat ttccgggtgc agcgcttctt tcatcgaacc
     3361 gataattttc agctcggtgc tttccgcgcc tttttccgcc acggtgtaca gcagttgctg
     3421 ttttttcagg ttttcgatca gcacgcggtc gttgcctggc atcaggttaa cttcgcagat
     3481 accccagtcg ctgtcggtgc tttccagcag atgatgggtg tacagcgcct ggtgcgcacg
     3541 gtgaaacgcc ccgcaaccga gatgcacaat gcgtgattcc agacgagaat gatcccatga
     3601 cgggcgggca accggcagat tgctgtcaac aatagtagtc atcgtaaact ccagaatccg
     3661 tcttcagaga gtgacggcaa ggaagaagga gccggcacgg cgcgcagcca tgccggtggg
     3721 tattaatggc taaagaaagc gcgctggatc gccagttcga caccgcgaac ttcggccagg
     3781 cctttcagac gaccaattgc ggagtaacca gggttggttt tcttcttcag gtcgtccagc
     3841 atctgatgac cgtggtccgg acgcatcggg atcaggtctt ctttgccttc cgctttacga
     3901 cggtgttctt cttcaacaat cgctttcacc acttcgtaca tatcaacgtc accgttcagg
     3961 tgcgccgctt cgtggaaggt tttcgggtta tcttcacgca tggtggagcg cagatgggtg
     4021 aagtaaatac gcggaccgaa ctgcttgatc atatcaacca aatcgttgtc agcacgcacg
     4081 ccgtaggaac cggtgcacat ggtgaaaccg ttcgccatgc tgtttacggt atcaaccatc
     4141 cactgcatat cttcaatggt ggaaacaatg cgcggcaggc cgaggatcgg gcgcggcgga
     4201 tcgtccgggt gaacagccat acggacgcca acttcttcgg caaccggaat aatcgctttc
     4261 aggaagacag caaagttttc gcgcagtttg gctttgtcga tatctttgta cagctccagg
     4321 tgcttacgga actggtcgag ggtgtaccct tcttccgcac ctggcagacc agcgatgatg
     4381 ttacgggtca gacgcgcttt gtcttcatcg ctcatagtgg cgaagcgttc agctgcctga
     4441 gcaatttctt cttcggtgta atccgcttcc gcgcctggac gtttcaggat atgcatttcg
     4501 aatgcagcga attcgatctg gtcgaagcgc agagctttgg agccgtctgg cagcacgtat
     4561 tcgaggtcag tacgggtcca gtcgagcacc ggcatgaagt tgtagcacac ggtgcgaatg
     4621 ccgcactgcg ccaggttgcg cagagtctgc tgatagttag cgatccactg ctcatagttg
     4681 ccagtgtggg ttttgatatc ttcgtgaatt ggtacgcttt ctacgacaga ccacaccagg
     4741 cctgcgtctt caacgatcgc cttgcgtttg aggatctctt ctacggacca tacttcgccg
     4801 ttcgggatat ggtgcagcgc ggtaaccacg ccagttgcgc ccgcctgacg gacatcagct
     4861 aaagaaaccg gatcgtttgg gccgtaccag cgccaggtct gttccatgat ttttcctctt
     4921 tcacatagtg ggttccacag caaagagacg cggaaccctg gagttgttta ggtatccgga
     4981 tgaacaacca gaccgcagcc gtggcgttgc atcggatgag atggcgtata agttctacct
     5041 tttggtaaga catgtcaacc cgaattctga aattggttaa ccacatcaca agaatttcac
     5101 tctcatggtt gttgctgcaa attgcagcaa agggtaatcg atggttattg atcagaggat
     5161 gtgacattca tcgcaacaat ggttgaccaa tttacataac atgtcgccca aaataacaac
     5221 ggttcaacca ggtaaggtaa aacgttatgc atgtgcttaa cattctctgg gtggtattcg
     5281 gcattggtct gatgctggta ctgaatttga agttcaaaat caattcaatg gtggctttgt
     5341 tggtggcggc gctgtccgtc gggatgctgg cgggcatgga tttgatgtcg ctgctgcaca
     5401 ccatgaaagc gggcttcggc aacacgctgg gggaactggc tatcatcgtg gtgttcggtg
     5461 cggtcatcgg taaattgatg gtcgactccg gcgcggctca ccagatagcg catacgctgc
     5521 tggcgcgtct cggtctgcgc tatgtacagc tgtcggtgat tatcatcggc ctgatttttg
     5581 gtctggcgat gttctatgaa gtggccttta tcatgttagc gccgctggtt attgtcattg
     5641 ccgccgaagc taaaattccg ttcctgaaac tggcgatccc ggcagtagca gctgccacta
     5701 ccgcacattc actgttccca ccgcagccgg gtccggtggc gctggtgaat gcttatggcg
     5761 cagatatggg gatggtttat atctatggcg tactggtgac gatcccaagt gtaatctgcg
     5821 caggtctgat cctgccgaag ttcctcggca atcttgagcg cccaacgcca tcattcctga
     5881 aagcagatca accggtagat atgaacaatc tgccctcttt cggcgtttcg attctggtgc
     5941 cgctgatccc ggcgatcatt atgatctcta ccaccatcgc caatatctgg ctggtaaaag
     6001 atacccctgc ctgggaagtg gttaacttta tcggttcctc gccgattgcg atgtttattg
     6061 cgatggtggt tgcattcgta ctctttggca ccgcgcgtgg tcatgacatg cagtgggtga
     6121 tgaacgcttt tgaaagcgcg gtgaagagta ttgcaatggt gattctgatc atcggtgcgg
     6181 gtggcgtgct gaagcaaact atcatcgaca ccggcattgg cgacaccatc ggcatgttga
     6241 tgtcccacgg caatatctcg ccctacatta tggcatggct gatcactgtg ctaattcgtc
     6301 tggcgacggg tcagggtgtc gtttcggcga tgaccgccgc cgggattatc agtgctgcaa
     6361 tccttgatcc agcaactggt cagctggttg gcgtgaatcc ggcgctgctg gtgctggcga
     6421 cggctgcggg ttccaacacc ctcacccaca ttaacgatgc atctttctgg ttgttcaaag
     6481 gttactttga cctgtcggta aaagacacgt tgaaaacctg gggtctgctg gagctggtca
     6541 actccgtggt tgggctgatt attgtgctga ttattagcat ggtagcgtaa aagaacacca
     6601 gagcctgcca atggcaggct cagttcggat tgtcggcaaa gtgcgctttg tttatgccag
     6661 atgcgacgct gacgcgtctt atctggccta caaagggcta acgtgcaggt ttttagcttc
     6721 aggtaatatt gcgtacctgc attagcaatg ccctgtgatt tctttattga taaacaaaag
     6781 tcacgccaat aatcgattgc acattccctg cagtcacctg cccgccggta cgtgcgtaat
     6841 ttgccgttaa tcccagactt accgccgaag ttcctactgc tcctaacgat accgtgttat
     6901 tcgctggaat aatcgtaccg ttgcgcgtca actgtacgcc gacgccctgc gctggtgaaa
     6961 acgacgcggt attggtgaaa atcgagttgc ccgcatctgc ggttgtgccg gagaggtaat
     7021 accccaggtt ttggcttttc gcacaataaa cggtaagagg aattggcact gaaccagggt
     7081 agtccggcag agtaacggtg acatcacgag cagaaacatc gcagccgcca gtaggcacta
     7141 ccacatcatt attggcgtaa atattccaca caaactggaa atcatcgctg ttatagtttt
     7201 tggtctgtcg caaaataagc acggcaatta atgagccagc tttaatcgcc accccgcccg
     7261 cactgctcac aggcgtcaaa taaagcgcca ccggccacgg cttatccgtt ctcgaattat
     7321 aaacaacccg cggcgtttcg ctggtggtcg gaaatggata gctactgcca ctatatttta
     7381 cggtcccgga aaaattagat aacacgccgc cataagccga gcctcgttgc agtgtgacat
     7441 agtctgtaat ggtttccgga taatcgttat ggcaaaagat ttgcgtcgaa agatctacga
     7501 ccaggttttg ccccacattc acggcaggcg caaggtttac ataaacatta gcgctgccac
     7561 cgccaatagg gatagcggta ccattggcgg ttttacaggc gaatgaccag gcatttaccg
     7621 accagcccat cagcagtaca gcaaacaggg taataactcg tttcattaca attatctctt
     7681 cgggttcagc tgtaggtata ggtgatgcta atcactgcct gaatggttcc ctgagtggct
     7741 ccgccattta ccgtcaatgc tctgacctgt aacgggaagt gcgctgattg tgaggaatca
     7801 tccacctgaa ctgttttggt tgcgccagta ttcaatgtgt tgccactgtc atcctgtagc
     7861 tctaactgga tgttttgcgc ggtcccctgg tttttataat atccggtact gtcggctgcc
     7921 ccgctgaagc tggcagtgac ccttgacgtt cccaccggac aattagtcaa ctcaagcgca
     7981 acatcatgcc aggccgatgc cgccccggca gacatcagac tgaaagaata aagatcgccg
     8041 agatcaaccg tggcattggt ggtggaaact gtgcacggtt tggcgacgac cttaccgttc
     8101 accgtgatgg tgacatcggc tgcctgtatc gtcgcacttg cgagcgccaa tattgccgcc
     8161 aataaatacc cacgtttgcg ccatttcatg agcatctcca gttactgata ttcaagagtg
     8221 aaggtagccg tggcattgat atgccccgca gtgacaggca cctgtgtcgc cattagccgg
     8281 gcgtaaaaat tcaacgtatt tggtttaccc ggcgtcaggg tcgtccacga aatcgcggac
     8341 gatggagcat taaggggtat ctgattttgc tgctcattca gaagctgtat tcccagtccc
     8401 gaagccgctg acaccgtatt ttcaagtgca agcaggttgg cattgtggct atctgcaacg
     8461 ccggtaaacc caacttttac ggcagaaacg gcgttaccac agggtgacag caaaatacga
     8521 aatggaacga caggagtcgt cgcgccaatg ttgttaaatt gcttcgccgc gttttccatc
     8581 agatcaacag taaaattggt tgattcagcg gccacactac agccgttatc tctgacatag
     8641 ccgcggatag taatcgtgct atccgcagcc aaagcgtgac tcactgccag ccacaaaaaa
     8701 gcgcacagaa gataaaaagg tttgtttctc atcacgcccc cttaacgaca ttcagctgat
     8761 agctgggtta ataactgctg ctgactctct ggtggcagtt gataattggc gacacaatga
     8821 gcattttcct cttctcccca tttcacctga acttttcccg ctagaggcat tccgctgagg
     8881 taaacctgac cattatccgc aacaatgccg ctactctggc tactctctga tgtcaccatc
     8941 gccccaaacg gcagcggctt attattgtgg gttagcgtca tgagcagttt tatcccaacg
     9001 cgcgctttaa actctgctcg cacgatcgcc ccacgagtgg gaacaacgtt agcgaccgcg
     9061 ttatctaaat cgacgttatc agccagggta ttggtatcca gcgccactct attttcccga
     9121 tattcagtgg cataaggcag cacggcataa ccgcgccagt cggtacgcac ccccgtctgg
     9181 ttttcgactt ttgcatcttt tgcgccaggc gctttaacaa gcaccaccgt atcgtttaac
     9241 ggctgcccca gcgttacgcc attggcatga gccagtaccc caccgctgac tccgtaatag
     9301 agctgcttaa tatcatcgct atggctgtaa ccgatattgg cattgccgta accaccgcga
     9361 taattcagcg tggcgtagcc tgtgcttccg ctattaccat cgcctccccc ggcatagccg
     9421 gtttgcacgc tatagctgag gttgttgtct tccagcaacg taccgtatac accagccaga
     9481 ttggtcatcc gaccgttgag atcgtgtgac atgctgtagc tggcactggc atgtcgccac
     9541 tgagatttac tgtcagaacg cagccagtgg ctgaaaggaa tattgacgtt acgcgctaac
     9601 atctgatcac gtcctttttg ccaggcgttt ttcgtcaggc tatagctgag cgtccagttg
     9661 atatcttcga acgcagtatt taatccagcc tggaattgct catcgacatt actcgttccc
     9721 caataagttt gatggctacc actcaaatac agtgttgatg agcgcccgag ttgctgagta
     9781 acggtgagtt gtaatttccc gcgtttgtta taagcgaggt tgtaatagtc ggtgaatttc
     9841 ggcttaacct gaataactcc gtcctgtgtt tcgatgttgt agccattcat tcgactgtat
     9901 gttgtatcag cgaaattaaa atatccgctg gtcgaataac ggtaacccac taactgaata
     9961 ttcgtgcctg actcattgag cgatttgtta tagagaaaac gcaccgattg tccgtcatgc
    10021 tgactgtcat cgggaagtgt ggaattagcc tgagtcatat ccacagacag agcgcccagt
    10081 gcccccatat ttttcccgat accaaaatta aaagcacgat aacgatctgc cagttgcgtt
    10141 ccaccatata ttgtccagcc agctggaagg ccgtggagta atgtactttg gaaaaagcgg
    10201 ggtttttcct gttgcgcatt tccactacgg tattctcctg ccgtaatgga ataacgagta
    10261 tgcccttcac gttgcaaaag cgggactgac gaatagggta cggtaaaaat ctgcgtgctg
    10321 ccgtcagcct ctttaatcgt tacctgcaag tcaccactat tacctgcggc atagatatcg
    10381 ttgatggtaa aaggccccgg cggcaccgta ctattataaa tgtcataccc attttgttta
    10441 atagtgacct gtgcagtacc acgagcaata ccgtggatca ccggggcaaa tcctctttgg
    10501 ctatcgggta acatattgtc atctgaggcc aattgtgcgc cgcgaaagtt aataccatcg
    10561 aaaatatcac cctgagtata accatcaccc agcgtcagcc gggaacgtaa cggtattatg
    10621 tctcgctcaa gccaggtatt gatatgctgc catttatttt tgctacctga tgatctgtcg
    10681 ctactgttat aactccaggt ggtattgtcg cgtaaacgcc acgcaccaat atttaaccca
    10741 ctctgtaggt ttaaatatgc ataatggctg ttacccccaa tccgattctg tacactattt
    10801 ccgctgaaat tataattgag caatccggca ttaataccgg gatcccataa ctcaggagga
    10861 atataaccac gcgcgcgatt actcataaat gcctgaggga tcgtcaggtt cagtcgctgc
    10921 tgaccaacat ctaaatgcgc agtagcgtcc tggaccattg tggttaatgg cacacaggca
    10981 tcatccgcca gcagattcat accggcgaca gaagccgtat tcagccccat actggcgagt
    11041 tgcgcgcgtg tcaggcaggg aacaatccct tgttcactgt cgcccgtatt aaatgtgaca
    11101 tcacgcgttg ccatataacc attattcaaa tagatatcga cgcgatacgt ccctggcggt
    11161 aattcttgcc cattttcaaa acgcgataaa tcggccacag cctggggatc atccgctaaa
    11221 aagcgcggat taaaatagag ttcggcagat gacaaaggtg cctgtacggc aaaagcacag
    11281 gcgacaaaga gccggacaaa aaaaccagcc aaacgatgct tacgaatatg caagcattgt
    11341 gtgtttcgct ggtaaagtct taaattcaga tatgacatta ttcctccggt ttaaaatcac
    11401 ccggcagtca attcttttta ttcaggcgaa aaattccccc tgcgttattc cattacgccc
    11461 gtcattttgg gggtaagtgc accataatca tttattgttc ggtaagtaat attgcttccc
    11521 gcatcagaag gcaatttaac cgtgctttcg cccattggag gcaccaaggc attttcaaga
    11581 acccgggctc cggcattcaa ctctgttacc gtcaggtaat agggtgtcgg gttaatcagc
    11641 gtcagagaat tcgcgctacg acgaaatctt aatttttctg cggcctgatc gggtggcaac
    11701 gctaatttag ccggacgata gtacagttta atgcggctga taattgcgag ctgtagcgta
    11761 ttctcagtca attttgattt atccattgac ggaatcgctt taacgttcat ccagaataaa
    11821 ctttcccggt cctgtggcaa ttggttattt gttgcatcaa gaatacgtaa ggtattctct
    11881 ttttttccct tcatcgcaaa cagaggaggc gtcacgataa aacgaccatc ctttacacca
    11941 tcggcatttt ccacccatga ttgaattaaa taggtactat tttcatcatt atttgtcacg
    12001 gcaagttgca cttgtttttg ccctgccgga taaattatgc gagtcgcacc taaggccact
    12061 cccgcttcag cgcgtccggc aaccatcatt gccatgaaca tcaggatacc tgccagcaag
    12121 cagaatgtta tttcctgcga tttccttaca ttgacgtttt tattactcac tgtcctgttc
    12181 ctgttatcac attatctgct gaacaattac tgataggtta aagagaacca ggcctgtgca
    12241 ttggcgatgc cgccagtaac ccgatgcccg gtagcacgat atttggcgat gaaatgtagc
    12301 gaagtagagc ctgagtaaag ccgtttccag tttgctggag gacgattaat cggtacgagg
    12361 tttccttcat catcaaacaa cgctacgcca atattggtgg ctatccctgg cccctctccc
    12421 acggaaagca catccggatt tttaccatcc gcgacaccgt gaaacgccac acccacacgt
    12481 tcactcacca ccgtgctaca ttcccgtaaa tgaataacaa aaggcaccgg tgcgctatct
    12541 tcccctaccg catgaaaccg gttactgctg atttgcccca tattgaccgt catttgttta
    12601 tcaccggctt caatcaggca agtttccgca ataatgacgc cctgaaattg catatttccg
    12661 ccgggcggcg tggtattcca tttatttccg gccagagcaa acattggcag caacgacgct
    12721 aatagaaata atctcttcct tttcatcgtt atttttatcg cacaagggtg ggcatccctg
    12781 cccgtaatga cgtccctgaa cctgggtagg ttattgatac tgaaccttga aggtcgcatc
    12841 cgcattagca gcacccgggg ttgcctcgcc gattgcataa taacgcgcct ggaacggaat
    12901 ggtgttagta ccgttattca gggttgtttg ctcactgaat gtcgcaccat ccagcgtcag
    12961 cgcagcaccc gttctgtcca ggatctgcac accaacgttt gttgcgctac ccgcagctga
    13021 actctgcaga gccagaacgt tggtatgacc cgcatcaatc gccgtaccta aaaaggcaac
    13081 agcggcttta gatgcaacat tggtatcgca atcattcagc tgaatgttaa aaccgacagc
    13141 agaactggtt gctccgtcct gtgccagcga tgcggtacga acctgtccta actgaacggt
    13201 ttgatcaaca gagcctgcat caactgcgca agcggcgtta acaacttccc ctttaaagtg
    13261 aacggtccca ccattaaccg tcgtggcagc ggccagagcc gctgtagaac tgagggacag
    13321 agccgacaga acaacgattg ccagagtttt aattttcatg ctgctttcct ttcaaaaaac
    13381 tatttctaaa tcgacatggg cagtcgttct gtacactttg ttttgtcaac gagtttgact
    13441 gccaacactg cacagttttc ccccaaaaga tgaaacattt ggggccattt tgactcatag
    13501 aggaaagcat cgcgcacaaa ctttttcagt ttatttgctg gattaatatt ctattattat
    13561 ctttatttat agatttttat attgcatgag gtggtttttg gagagaagaa tgaggaagat
    13621 gcgtcgagcc acagaaacgt
//
LOCUS       DAZKIF010000119        13619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZKIF010000119 DAZKIF010000000
VERSION     DAZKIF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.120"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            147..1256
                     /gene="livK"
                     /locus_tag="ACHOTW_003689"
     CDS             147..1256
                     /gene="livK"
                     /locus_tag="ACHOTW_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP9845723.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1304..2230
                     /gene="livH"
                     /locus_tag="ACHOTW_003690"
     CDS             1304..2230
                     /gene="livH"
                     /locus_tag="ACHOTW_003690"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP9845724.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2227..3504
                     /gene="livM"
                     /locus_tag="ACHOTW_003691"
     CDS             2227..3504
                     /gene="livM"
                     /locus_tag="ACHOTW_003691"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP9845725.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3501..4268
                     /gene="livG"
                     /locus_tag="ACHOTW_003692"
     CDS             3501..4268
                     /gene="livG"
                     /locus_tag="ACHOTW_003692"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP9845726.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4270..4983
                     /gene="livF"
                     /locus_tag="ACHOTW_003693"
     CDS             4270..4983
                     /gene="livF"
                     /locus_tag="ACHOTW_003693"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP9845727.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5102..5848
                     /locus_tag="ACHOTW_003694"
     CDS             5102..5848
                     /locus_tag="ACHOTW_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9845728.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            6208..7524
                     /gene="ugpB"
                     /locus_tag="ACHOTW_003695"
     CDS             6208..7524
                     /gene="ugpB"
                     /locus_tag="ACHOTW_003695"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP9845729.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            7622..8509
                     /gene="ugpA"
                     /locus_tag="ACHOTW_003696"
     CDS             7622..8509
                     /gene="ugpA"
                     /locus_tag="ACHOTW_003696"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP9845730.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            8506..9351
                     /gene="ugpE"
                     /locus_tag="ACHOTW_003697"
     CDS             8506..9351
                     /gene="ugpE"
                     /locus_tag="ACHOTW_003697"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP9845731.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9353..10423
                     /gene="ugpC"
                     /locus_tag="ACHOTW_003698"
     CDS             9353..10423
                     /gene="ugpC"
                     /locus_tag="ACHOTW_003698"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP9845732.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10420..11163
                     /gene="ugpQ"
                     /locus_tag="ACHOTW_003699"
     CDS             10420..11163
                     /gene="ugpQ"
                     /locus_tag="ACHOTW_003699"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9845733.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11150..11590)
                     /locus_tag="ACHOTW_003700"
     CDS             complement(11150..11590)
                     /locus_tag="ACHOTW_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP9845734.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            11710..13455
                     /gene="ggt"
                     /locus_tag="ACHOTW_003701"
     CDS             11710..13455
                     /gene="ggt"
                     /locus_tag="ACHOTW_003701"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP9845735.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT         3021 a         3456 c         3901 g         3241 t
ORIGIN      
        1 agggacttta atcgctatta tcacaaaata ctgcgctaaa cccttaatca gacaggcaaa
       61 aacagtgcag tataaaaaaa gaacagtctg atttgttaac acataaaaac aaagcaacac
      121 aacatcacga atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga
      181 tgattgcact ggcaatttca cacaccgctt tagccgacga tattaaagtt gccgttgtcg
      241 gcgcgatgtc cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg
      301 cgattaaaga cattaatgcc aaagggggaa ttaaaggcga taagctggtt ggcgtagaat
      361 atgacgacgc ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaatgacg
      421 gcattaaata cgttattggt catctgtgtt cttcttctac ccagcctgcg tcagatatct
      481 atgaagacga aggtattctt atgatctcgc cgggagcgac aaacccggag ctgacccaac
      541 gcggttatca acacattatg cgtactgccg ggctggactc ttcccagggg ccaacggcgg
      601 caaaatacat tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac
      661 agtatggcga agggctggcg cgttcggtgc aggacgggct gaaagcggct aacgccaacg
      721 tcgtcttctt cgacggtatt accgccgggg agaaagattt ctccgcgctg atcgcccgcc
      781 tgaaaaaaga aaacatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga
      841 tgctgcgcca ggcccgttcc gttggcctga aaacccagtt tatggggccg gaaggtgtgg
      901 gtaatgcgtc gttgtcgaac attgccggtg atgccgccga aggcatgttg gtcactatgc
      961 caaaacgcta tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca
     1021 agaaagatcc gtccgggcct tatgtctgga tcacctacgc ggcggtgcaa tctctggcaa
     1081 ctgcccttga acgtaccggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta
     1141 acggtgcaaa caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat
     1201 ttgattttgg tgtcttccag tggcacgccg acggttcatc cacggcagcc aagtgaaaat
     1261 cccaccgccc gtgaaatgcg ggcgggttta gaaaggttac cttatgtctg agcagttttt
     1321 gtatttcttg cagcagatgt ttaacggcgt cacgctgggc agtacttacg cgctgattgc
     1381 catcggctac accatggttt acggcattat cggcatgatc aacttcgccc acggcgaggt
     1441 ttacatgatc ggcagctacg tctcatttat gatcatcgcc gcactgatga tgatgggcat
     1501 tgataccggc tggctgctgg tagcggcggg attcgtcggc gcaatcgtca ttgccagcgc
     1561 ctacggctgg agtatcgaac gggtggctta ccgcccggtg cgtaactcta agcgcctgat
     1621 tgcactcatc tcggcaatcg gtatgtccat cttcctgcaa aactacgtca gcctgactga
     1681 aggttcgcgc gacgtggcgc tgcccagctt gtttaatggt cagtgggtag tgggtcatag
     1741 cgaaaacttc tctgcctcta ttaccaccat gcaggcggtg atctggattg tcaccttcct
     1801 cgccatgctg gcgctgacga ttttcattcg ttattcccgt atggggcgcg cctgccgtgc
     1861 ctgcgccgaa gatctgaaaa tggcgagcct gcttggcatt aacaccgacc gggtgattgc
     1921 tttgaccttt gtgattggcg cggcgatggc ggcggtggcg ggcgtactgc tcggtcagtt
     1981 ctacggcgtc attaacccct atatcggctt tatggccggg atgaaagcct ttaccgctgc
     2041 ggtgctcggt gggattggca gcattccggg agcgatgatt ggcgggctga ttctggggat
     2101 tgcggaggcg ctctcttctg cctacctgag tacggaatat aaagatgtgg tctcattcgc
     2161 cctgctgatt ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga
     2221 gaaagtatga aaccgatgca tattgcaatg gcgttgctct ctgccgcgat gttttttgtc
     2281 ctggcgggcg tctttatggg cgtacaactg gagctggatg gcaccaaact ggtggtcgac
     2341 acggcttcag acatccgttg gcagtgggtg tttatcggca cggcggtggt ctttttcttc
     2401 cagcttttgc gaccggcttt ccagaaaggg ttgaaaagcg tttccggacc gaagtttatt
     2461 ctgcccgcca ttgatggctc cacggtgaag caaaagctgt ttctcgtggc gctgctggtg
     2521 cttgcggtgg cgtggccgtt tatggtctca cgcgggacgg tggatatcgc caccctgacc
     2581 atgatctaca ttatcctcgg actcgggctg aacgtggtgg tagggctttc tggtctgctg
     2641 gtgctggggt acggcggctt ttacgccatc ggcgcttata cctttgcgct gctcaatcac
     2701 tattacggct tgggcttctg gacctgtctg ccgatagccg ggttgatggc ggcggcggcg
     2761 ggcttcctgc tcggcttccc ggtgcttcgc ttgcgcggtg actatctggc gatcgttacc
     2821 ctcggtttcg gcgaaattgt gcgcatattg ctactcaata acaccgaaat taccggcggt
     2881 ccgaacggta tcagtcagat cccgaaaccg acttttttcg gccttgagtt cagccgtacc
     2941 gctcgtgaag gcggctggga cacgttcagt aatttctttg gcctgaaata cgatccctcc
     3001 gatcgtgtca ttttcctcta cctggtggcg ttgctgctgg tggtgctaag cctgtttgtc
     3061 attaaccgcc tgctgcggat gccgctgggt cgcgcctggg aagcgttgcg tgaagatgaa
     3121 atcgcctgcc gttcgctggg cttaagcccg cgtcgtatca agctgactgc ctttaccatc
     3181 agcgccgcgt ttgccggttt tgccggaacg ctgtttgctg cgcgtcaggg ctttgtcagc
     3241 ccggaatcct tcacctttgc cgaatcggcg tttgtgctgg cgatagtggt gctcggcggt
     3301 atgggctcgc aatttgcggt aattctggcg gcaattttgc tggtggtgtc gcgcgagttg
     3361 atgcgtgatt tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg
     3421 atctggcgtc cgcagggctt gctgcccatg acgcgcccgc aactgaagct gaaaaacggc
     3481 gcagcgaaag gagagcaggc atgagtcagc cattattatc tgttaacggc ctgatgatgc
     3541 gcttcggcgg cctgctggct gtgaataacg tcaatcttga attgtacccg caggagatcg
     3601 tctcgttaat cggtcctaac ggtgccggaa aaaccacggt ctttaactgc ctgaccggat
     3661 tctacaaacc caccggcggc actattttac tgcgcgatca gcatctggag ggtttgccgg
     3721 ggcagcaaat cgcccgcatg ggcgtggtgc gtaccttcca gcatgtgcgt ctgttccgcg
     3781 aaatgacggt aattgaaaac ctgctggtgg cgcagcatca gcaactgaaa accgggctgt
     3841 tctctggcct gttgaaaacg ccttctttcc gtcgcgccca aagtgaagcg ctcgaccgcg
     3901 ccgccacctg gcttgagcgc attggtttgc tggaacacgc caaccgtcag gcgagtaacc
     3961 tggcctacgg tgaccagcgc cgtctggaga ttgcccgctg catggtgacg cagccggaga
     4021 ttttaatgct cgacgaacct gcggcaggtc ttaacccgaa agaaacgaaa gagctggatg
     4081 agctgattgc cgaactgcgc aatcatcaca acaccactat cttgttgatt gaacacgata
     4141 tgaagctggt gatgggaatt tcggaccgaa tttacgtggt caatcagggg acgccgctgg
     4201 caaacggtac gccggagcaa atccgtaata acccggacgt gatccgtgcc tatttaggtg
     4261 aggcataaga tggaaaaagt catgttgtcc tttgacaaag tcagcgccca ctacggcaaa
     4321 atccaggcgc tgcatgaggt gagcctgcat atcaatcagg gcgagattgt cacgctgatt
     4381 ggcgcgaacg gggcggggaa aaccaccttg ctcggcacgt tatgcggcga tccgcgcgcc
     4441 accagcgggc gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc
     4501 atgcgcgaag cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg
     4561 gaagagaacc tggcgatggg cggctttttt gccgaacgtg atcagtttca ggagcgcata
     4621 aagtgggtgt atgagctgtt tccacgtctt catgagcgtc gtgttcagcg ggcaggcacc
     4681 atgtccggcg gtgaacagca gatgctggcg attggtcgcg cgctgatgag caatccgcgt
     4741 ttgttgttgc ttgatgagcc atcgctcggt cttgcgccga ttatcatcca gcaaattttc
     4801 gacaccatcg agcagctgcg cgagcagggg atgactatct tcctcgtcga gcagaacgcc
     4861 aaccaggcgc tgaagctcgc tgatcgcggc tacgtgctgg aaaacggcca tgttgtgctc
     4921 tccgatactg gtgatgcgct gctggcgaac gaagcagtga gaagtgcgta tttaggcggg
     4981 taaattatcg ataacccggc agctaagttc tgccgggtta ttcgtccgta tacagacttg
     5041 tttcaactga ccgggaggat taaaaccaga ccacttattt gccacggaaa ggttccctcc
     5101 catgcattat gccgttgttg tcatgcttga actgctttgc tgtattcacg cttaccgtac
     5161 cggacaagag cggtactgga tttttatcat tttctgtttt ccggtgattg gctgcgtggc
     5221 gtatttcgtc atagtgatgc tgcctgaaac gggcgctgac cgtcacgggc atactttgct
     5281 gatgcgcctg caggacaaac tcaaccctga gcgccacctg cgcaaattaa ccgaagaact
     5341 ggcgattgcc gagacgaatc aaaaccatta tgcgttggct aatgaactgg cgcgtctggg
     5401 gcgttatcac gaagctgttc cccattatca gcaggcgtta agcgggatat tcgcccatga
     5461 agcggcaatg atgctaagcc tggcgcaggc acagtttgct attcaggagt tcgccgcctg
     5521 tcagcagacg ctggaagatg tgatgcgtta taaccctgat tttcagtcgg cagacgggca
     5581 tttgttattt gccagaacgt tagccgcaca agaaaaatac gctgatgcgg aaagcgagtt
     5641 tgaagtattg atttcttatt accccggccc gcaggcgcgt atttactatg cggaaatgtt
     5701 ggcgaaaatg agtcggttac gtgaagccaa cgagcagtat gttgccgtgg ttgatacggc
     5761 taaacgctca agacctcatt atagaaaaca ccaccgggaa tggattaaga ccgccaatga
     5821 gcggctgaag cagagtgtgg tgcaatgaaa atcttctttg ccgttagacc agtgtcgcgg
     5881 caatcttttg ttaaaattga aaaatgtagc tctcatcggg cagcaaaatt ggacgcagaa
     5941 tttgctgcca tttttgctct tcatggtaag gagttctggc gattagctgg ccgttaatcg
     6001 cctcttttca gtgcattctc gggatatccg tgccccatct cccattttcc ctcacccgta
     6061 ccgtcaccgc cttgtcatct ttctgacacc ttactatctt acaaatgtaa caaaaaagtt
     6121 atttttctgt aattcgagca tgtcatgtta ccccgcgagc ataaaacgcg tgaattcgcg
     6181 cattcggtac aacaagagag ataaacgatg aaaccgttac attatacagc ttcagcactg
     6241 gcgctcggac tggcgttaat ggggaatgca caggcagtga cgaccattcc gttctggcat
     6301 tctatggaag gggaactggg taaagaggtg gattctctgg cccaacgttt taacgccgaa
     6361 aacccggatt acaaaattgt accgacctat aaaggcaatt acgaacagaa tttaagcgcg
     6421 gggattgccg catttcgtac cggcaatgct ccggctattt tgcaggttta tgaagttggc
     6481 accgccacca tgatggcgtc gaaagccatt aaaccggttt atgacgtgtt taaagaggcg
     6541 gggattcaat tcgatgagtc gcagtttgtg ccgacggttt ccggttacta ctccgacagc
     6601 aaaacgggcc acttactctc ccagccattc aacagctcga ctcccgttct ctattacaac
     6661 aaagacgcct tcaagaaagc ggggttagac ccggaacaac cgccgaaaac ctggcaggat
     6721 ctggcggact atgccgcaaa actaaaagcc tccggcatga agtgcggcta cgccagcggc
     6781 tggcagggct ggatccaact ggaaaacttt agcgcctgga acggtctgcc gtttgccagc
     6841 aaaaacaacg gctttgacgg cacggatgcg gtgctggagt tcaacaagcc ggagcaggtg
     6901 aaacacatcg ccatgctcga agagatgaac aagaagggcg atttcagcta cgtcgggcgt
     6961 aaggatgaat ccaccgagaa gttctataac ggtgattgcg ctatgaccac cgcctcttcc
     7021 ggttctctcg ctaatattcg cgagtacgcc aaattcaact atggcgtagg catgatgcct
     7081 tacgacgccg atgcgaaaga tgcaccgcaa aacgccatta tcggcggggc cagcctgtgg
     7141 gtgatgcagg gcaaagataa agaaacgtat accggcgtgg cgaagttcct cgacttcctc
     7201 gcgaagccag aaaacgctgc cgagtggcat cagaaaaccg gttatctgcc aatcaccaaa
     7261 gcagcgtatg acctgacccg tgagcagggc ttttatgaga aaaacccagg ggcggatacc
     7321 gcgacgcgtc agatgctgaa taagccgccg ttgccgttca ccaaagggct gcgtctgggc
     7381 aacatgccgc agatccgcgt gattgtggat gaagagctgg agagcgtgtg gaccggtaag
     7441 aagacaccac agcaggcact ggataccgcc gttgagcgtg gaaatcagtt gctgcgccgc
     7501 tttgagaaat cgacgaagtc ttaatcagtg taatgtcgga tgcgtttcgc ttatctgacc
     7561 tggcatcgcg tgtaggccgg ataagcgaag cgcatccggc acagttcagg aattaaccgt
     7621 aatgtcatca tcccgtccgg tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc
     7681 gcagctcatc atcaccgtta tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc
     7741 gctgcaaagc gtcgatccgt ttggtttctc cagccggttt gtcggcctgg ataacttcgt
     7801 cgcgctgttt catgacagct actatatcga ctccttctgg acgacgataa aattcagcac
     7861 ctttgtcacc gtcagcggtt tgctggtatc gctgttcttt gcggcgctgg tggagtacat
     7921 cgtgcgcggc agtcgtttct atcaaacctt gatgctgctg ccgtatgccg tggctcccgc
     7981 cgttgccgcc gtattgtgga tcttcctgtt taaccccggt cgcgggctga tcacccattt
     8041 tctcgctgag ttcggctacg actggaacca cgcgcaaaac agcggtcagg caatgtttct
     8101 ggtggtgttt gcctcagtat ggaagcaaat cagctacaac ttcctgttct tctatgccgc
     8161 gctgcaatcc attccccgtt cgttgatcga agccgcagcc atcgacggtg cagggccgat
     8221 tcgccgcttc tttaagattg cgctgccgct tatcgccccg gtgagtttct tcctgctggt
     8281 agtgaacctg gtgtatgcct tcttcgacac cttcccggtg atcgacgccg ccacgtccgg
     8341 cgggccggtt caggccacca ctacgctgat ttataagatc taccgcgaag gctttaccgg
     8401 actggatctg gcttcgtctg ccgcacagtc ggtggtgttg atgttcctcg tcatcgtgct
     8461 gacggtgatg cagttccgct atgttgaaag caaggtgcgt taccaatgat tgagaaccgt
     8521 ccgtggctga cgatattcag ccataccatg ctgatcctcg ggatcgcggt gatcctattc
     8581 ccgctgtacg tggcgtttgt cgcggcgacg ctggataaac aggccgtcta cgccgcgccg
     8641 atgacgctca tccccggcac gcatctgctg gaaaacatcc acaacatctg ggtaaacggg
     8701 gtaggcacaa atagtgcgcc gttctggcgg atgttgctta acagcttcgt gatggcgttc
     8761 agcattacgc tcggcaaaat taccgtctcg atgctctcgg cattcgccat tgtctggttt
     8821 cgttttccgc tgcgtaacct ctttttctgg atgattttta tcaccctgat gttgccggtt
     8881 gaagtacgta tcttcccgac ggtggaagtc atcgccaacc tgaagatgct cgacagctac
     8941 gcaggtttaa cgctgccgct gatggcctcg gcgaccgcga cgtttctttt ccgccagttc
     9001 tttatgaccc taccggatga actggtggaa gccgcgcgga tcgacggcgc gtcgccaatg
     9061 cgcttctttt gcgacatcgt ttttccgttg tccaaaacta atctggcggc gttgtttgtg
     9121 atcaccttta tttacggctg gaaccagtat ttgtggccgt tgttgattat taccgacgtg
     9181 gatctcggca ccaccgtggc agggatcaaa gggatgatcg ccactggcga aggcaccaca
     9241 gaatggaact cagtgatggc ggcgatgttg ttaaccctga tcccaccagt agtgattgtt
     9301 ttagtgatgc agcgtgcctt cgtgcgcggc ctggtcgata gtgagaaata agatggcagg
     9361 actgaaatta caggcagtaa ccaaaagctg ggatggcaaa acccaggtga ttaaaccgct
     9421 gacccttgat gtggcggatg gcgaatttat cgtgatggtc gggccgtcag gctgcgggaa
     9481 atcgacgctg ctgcgcatgg ttgccgggct ggagcgggtg acggaaggcg atatctgtat
     9541 caacgaccag cgggtgaccg aaatggagcc gaaagatcgc gggattgcga tggtgttcca
     9601 gaactacgcg ctttatccgc atatgagtgt tgaagaaaac atggcgtggg ggctgaaaat
     9661 tcgcggcatg ggcaagcagc aaattgccga gcgcgttaaa gaggcggcgc gcattctgga
     9721 actggacggt ctgcttaagc gccgcccgcg cgagctttcc ggcggtcagc gtcagcgtgt
     9781 ggcgatgggc cgggcgattg tgcgcgatcc ggcggtgttc ctgtttgatg agccactctc
     9841 taacctcgat gccaagctgc gcgtacagat gcgtcttgaa ctgcaacagc tgcaccgtcg
     9901 cctgaaaacg acttcactct acgttactca cgatcaggtt gaggcgatga ccctcgccca
     9961 gcgagtaatg gtgatgaacg gcggcgttgc cgaacagatt ggcacaccag ttgaagtcta
    10021 cgaaaagccc gccagcctgt ttgtggcgag ttttattggc agcccggcga tgaatctgct
    10081 gacaggccgc gtgaataacg aaggcacgca cttcgagctg gacggcggca ttgcgctgcc
    10141 gctaaacggt ggctaccgtc agtatgcagg gcgtaaaatg actctcggca ttcgcccgga
    10201 acatatcgcg ctaagctcgc aggcagaagg cggcgtgccg ctggtgatgg acacgctgga
    10261 gatcctcggc gcagataacc tggcgcacgg acgctggggc gaacagaagc tggtggtacg
    10321 gctggcgcat caggagcgcc cgacggcagg cagcacgctg tggctgcatc tgccggaaaa
    10381 tcagctacat ctttttgatg gtgaaacagg acaacgagta tgagtaactg gccttatccc
    10441 cgcatcgtcg cccaccgtgg cggcggtaaa ctggccccgg aaaacaccct ggcggcaatc
    10501 gacgtcgggg caaaatacgg tcataagatg atcgaatttg acgcgaagtt gtcgaaagat
    10561 ggcgaaatct tcctgctcca tgacgacaat ctcgaacgta ccagcaacgg ctggggcgtc
    10621 gcgggtgaac tgaactggca ggatttacta cgtgtggatg caggcagttg gtacggcaaa
    10681 gcgtttaaag gtgagccgct gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacat
    10741 gggatgatgg cgaatatcga aatcaaaccc accactggca ctgggccatt aacgggcaaa
    10801 atggtggcgc tggcggcacg cgagctgtgg gccggtatga cgccgccgct actgtcatcg
    10861 tttgagattg atgctttaga agccgcacaa caggcagtac cagaactgcc gcgcggtttg
    10921 ttgctggatg agtggcgcga cgactggcga gaactgaccg cgcggctggg ctgcgtctct
    10981 attcatctca atcataagtt gctcgataaa gcgcgagtga tgcagttgaa agacgccgga
    11041 ttacggattc tggtttatac cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg
    11101 ggcgtggatt gcatctgtac cgatgcgatt gatgtgattg gtccgaactt tacggcccaa
    11161 tagttttcaa cggtatatca ggctgcggcg tgccgggctg attcagcatg tcgccgtttc
    11221 tcctctccgg caacatatgc tgctgattca acgaactatc cggattacgg ttgctgttta
    11281 acatcccgcc attggtgttg ggcagcattt gctgccgggc gggatttcgc tcccccggct
    11341 gtgactgcaa cacccgctga gaattattgt ttatctggtt ttctaaatgc tgttgttgca
    11401 actgagtttg cgttttcagt tgctgattca gcatcccttt ttgctggatt tgctgcgttt
    11461 gcatctgggt ttgcatccgc tgctgactgg ggatctgata ccccggctgg ttagggttgt
    11521 tcagagtatt aatgggctgt gcaaagccga caaacggcag gagtgccgta agaatcagaa
    11581 gtcgtttcat cgcgtatcct cctctgaaga tattctttaa gtttactcct ttcctgcaat
    11641 catcaggatt aattcagagt tatataccag gcttagctgg ggttgcccct taatctctgg
    11701 agaataacga tgataaaacc gacgttttta cgccgggtgg ccattgctgc tctgttcaca
    11761 ggaagttgtt ttagtacggt agccgcgcca cctgcgccgt ctcctgtttc ttacggcgtg
    11821 gaggaagatg tctttcatcc ggtacgcgcg aaacagggga tggtggcatc tgtagatgct
    11881 actgccactc aggtgggggt ggatatcctc aaggagggcg ggaatgccgt tgatgccgcc
    11941 gtggctgtgg gctacgcgct ggcggtaacg catccgcagg cagggaatct gggcggtggt
    12001 ggttttatgc tgatccgctc gaaaaatggc aataccaccg ctatcgattt ccgcgaaatg
    12061 gcacccgcca aagcgacccg cgatatgttc cttgatgacc agggcaaccc ggacagcaaa
    12121 aaatcactca cttcgcatct ggcttccggc acgcccggta cggtcgctgg tttctcgctg
    12181 gcgttggata aatacggcac aatgccgttg aacaaagtgg tgcagccagc gtttaaactg
    12241 gcacgcgatg gttttatcgt taacgacgag ctggctgacg atctcaaaac ctacgggagc
    12301 gaagtgctgc cgaatcacga aaacagcaag gctatcttct ggaaagaggg cgagccgctg
    12361 aaaaagggcg acaagctggt gcaggcgaac ctggcaaaga gcctggagat gattgccgaa
    12421 aacggcccgg acgaattcta caaaggcacg atagcggaac agattgccca ggagatgcag
    12481 aaaaacggtg gcctgatcac taaagaagat ttagcggcgt ataaagcggt cgaacgcaca
    12541 ccgataagcg gcgattatcg cgggtatcag gtttactcca tgccaccgcc atcctccggc
    12601 gggatccata tcgtacaaat cctcaatatt ctggaaaact tcgatatgaa gaaatacggt
    12661 tttggcagcg ccgacgcgat gcagatcatg gcagaagcgg agaaatatgc ctacgctgac
    12721 cgctcggaat atcttggcga cccggatttt gtgaaagtcc cgtggcaggc gctgaccaat
    12781 aaagcctatg ccaaatctat tgccgagcaa atcgatatca ataaagcgaa accgtccagc
    12841 gagattcgcc ccggcaagct tgcgccttat gagagtaatc aaactaccca ttattcggtg
    12901 gtggataaag acggtaacgc ggtggcggtg acctatacgc tgaacaccac cttcggtacg
    12961 ggtattgtcg cgggcgagag cggtattttg ctcaataacc aaatggatga tttctctgcc
    13021 aaaccgggcg taccgaacgt ttacgggctg gtgggcggtg atgccaacgc cgtcgggccg
    13081 aacaaacgcc cgctgtcatc gatgtcacca accattgtgg tgaaagacgg taaaacctgg
    13141 ctggtcaccg gtagccctgg cgggagcctg atcatcacta cagtgcttca aatggtggtg
    13201 aatagcatcg attatggcat gaacgtcgcc gaagcgacca atgcgccgcg tttccaccat
    13261 cagtggttgc cggacgagct gcgtgtcgaa aaagggttta gcccggatac gctcaagctg
    13321 ctggaagcaa aaggtcagaa agtggcgctg aaagaggcga tgggcagtac acaaagcatt
    13381 atggttgggc cggacggtga gttgtacggc gcatccgacc cgcgctcggt ggatgattta
    13441 acggcggggt actaaggtta gcggccctct tcgtgggaag agggctgttt tcttgaacaa
    13501 gataaattag tgtctggatt gcttttacta ttgctccgta atatataatc ataattatcc
    13561 tgaatgcttt taaggggctt aggttatgct ttcttttcaa gagtatgcag aatagtctt
//
LOCUS       DAZKIF010000120        13437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZKIF010000120 DAZKIF010000000
VERSION     DAZKIF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.121"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(82..234)
                     /locus_tag="ACHOTW_003702"
     CDS             complement(82..234)
                     /locus_tag="ACHOTW_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9845736.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(498..1232)
                     /gene="cysH"
                     /locus_tag="ACHOTW_003703"
     CDS             complement(498..1232)
                     /gene="cysH"
                     /locus_tag="ACHOTW_003703"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9845737.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1306..3018)
                     /gene="cysI"
                     /locus_tag="ACHOTW_003704"
     CDS             complement(1306..3018)
                     /gene="cysI"
                     /locus_tag="ACHOTW_003704"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP9845738.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3018..4817)
                     /gene="cysJ"
                     /locus_tag="ACHOTW_003705"
     CDS             complement(3018..4817)
                     /gene="cysJ"
                     /locus_tag="ACHOTW_003705"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP9845739.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5136..5498
                     /gene="queD"
                     /locus_tag="ACHOTW_003706"
     CDS             5136..5498
                     /gene="queD"
                     /locus_tag="ACHOTW_003706"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP9845740.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5576..6847
                     /gene="ygcN"
                     /locus_tag="ACHOTW_003707"
     CDS             5576..6847
                     /gene="ygcN"
                     /locus_tag="ACHOTW_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9845741.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            6838..7098
                     /gene="ygcO"
                     /locus_tag="ACHOTW_003708"
     CDS             6838..7098
                     /gene="ygcO"
                     /locus_tag="ACHOTW_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9845742.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7115..7690
                     /gene="ygcP"
                     /locus_tag="ACHOTW_003709"
     CDS             7115..7690
                     /gene="ygcP"
                     /locus_tag="ACHOTW_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP9845743.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7838..8698)
                     /gene="ygcQ"
                     /locus_tag="ACHOTW_003710"
                     /pseudo
     CDS             complement(7838..8698)
                     /gene="ygcQ"
                     /locus_tag="ACHOTW_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(8695..9474)
                     /gene="ygcR"
                     /locus_tag="ACHOTW_003711"
     CDS             complement(8695..9474)
                     /gene="ygcR"
                     /locus_tag="ACHOTW_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP9845744.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9452..10788)
                     /gene="ygcS"
                     /locus_tag="ACHOTW_003712"
                     /pseudo
     CDS             complement(9452..10788)
                     /gene="ygcS"
                     /locus_tag="ACHOTW_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10882..12336)
                     /gene="ygcU"
                     /locus_tag="ACHOTW_003713"
     CDS             complement(10882..12336)
                     /gene="ygcU"
                     /locus_tag="ACHOTW_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP9845745.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12406..13191)
                     /gene="ygcW"
                     /locus_tag="ACHOTW_003714"
     CDS             complement(12406..13191)
                     /gene="ygcW"
                     /locus_tag="ACHOTW_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9845746.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
BASE COUNT         3145 a         3664 c         3367 g         3261 t
ORIGIN      
        1 taaaaaatat aaatttaaaa aagggtacag gagaccaaac catcacaatt caatacgcgg
       61 ggttttaccc ccgcgtggtg gctacttctt cgattcgtaa gcgagaactg ccttaaactc
      121 cataccacgc tctctgatac tcaactcaca taacgagtcg cctaccatga gcgtgaatcc
      181 cagtactgta caacacagta ctatgattgc cacaagggca tattttgtta gcatgtcttg
      241 cctccctgca aagaagagac taacatccga attgctacgt tcgaacgcga gcctcaggtt
      301 gatagaaata tcgcctgggg cttttgtcca tctggaacct cgcgaatgct taacgccaga
      361 cagcctcaag cacccgacgc cattctatac ctgataattc ttccagcggc gttcacgttt
      421 aaaatcagga atgctgctcg caaaataaaa acgcccccat cattactgac agaggcgttt
      481 aatttatccg gcaatattta cccttcgtgt aacccacatt ccctttttaa gccaaagaaa
      541 cgcgtttctt cttccgccat gccgggttcc catttacggg ttgtatgggt atcgcccacc
      601 gataaatatc cttcgtccca taatgggtga tatttcaggc cattcttttg cagatactga
      661 tatatcgttc ggttatccca gtcgataatc ggcagcactt taaatacgcc acgctgaatt
      721 gccagcaccg gtaaattggc gcgactgccg gattgttcac ggcgcaggcc agcaaaccag
      781 gtttgcgcgt taagttcttt cagcgcccgg ttcatcggtt cgactttgtt gatgtcattg
      841 tacttttcaa tgccttcaac gccctgttcc cacagttttc cgtagcgtgc ttcctgccag
      901 gctgcgcttt cggtagcacg gtacactttc aggttgagct tgagtttgtc cgttaactcg
      961 tcaataaagc ggtaggtttc cgggaacaag taacccgtat cggtgaggat caccggaata
     1021 tccgggcgaa tttgattcac cagatgcagg ctcaccgccg cctgaatgcc aaagctggaa
     1081 gaaagtacat attcaccggg cagattatcc agcgcccagg ctacgcggcc ttcagcgtcc
     1141 agtttttcca gttgggcgtt agtttccgcc agcgccagaa tgcgatctac cttcggcagt
     1201 tcgttcaggg cgtttagatc gagtttggac atagattcct cactgtttgc cttgcctgat
     1261 gcgacgctca cgcgtcttat caggcctaca agaccgggct gatggttaat cccacaaatc
     1321 gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc
     1381 ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat
     1441 ttccggctcg gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc
     1501 gccaagatgc aggttgtagc gacccggcgc tttacccacc aggcccactt ccgccagcat
     1561 cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac
     1621 accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctccgc
     1681 ttccgccatc gccagcgggc aagtcgggaa cgagacgcag gccattgagt tttcacgctg
     1741 cggcgtgacg gcattcatta acccgctctc tttggcgatc ttctcgatct tcgctttctc
     1801 gctttccggc acaccggcga tgatcagatt ctggttcgcc gtaatgcgga aatcaccttt
     1861 gtggatcttc gcgatctcca gcaggccggt tttcagcgga cgccccggat aatcaaggat
     1921 gcgaccattt tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc
     1981 aatacgatct ccgcgtccgg tgaactcata cggacggatc ggttcaaatt tgatccccgc
     2041 gcgacgctcc acttccgctt taaacgtctc aacccccacg cgctccagcg tgtatttggt
     2101 tttggcattt ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc
     2161 cgccactgcc agcgtatgct ccagcggcag atagccaaac tcactcgccg tgcgggcgta
     2221 ggttttcttg ttgccgtgtt cgatagaaag tccaccgccc accaacaggt taaagcccac
     2281 cagcttgccg ttttcggcga ttgccacgaa gttcatgtcg ttggcgtgca gatcgatatc
     2341 gttctgtggc gggatcacta ccgtggtttt gaatttacgc ggcaggtagg tctggccgag
     2401 gatcggttct tcatcggtgg tggcgacttt ttcctgatcg agccagatct ccgcatacgc
     2461 gcgggtacga ggcagcagat gttcagagat tttcttcgcc cactcgtacg cttctgcgtg
     2521 cagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttagcggt
     2581 cgccagcgca tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt
     2641 cagaatgccg tggaactgga atgtctggcg gttggtcagg cgaatgctgc catagatggt
     2701 gttttcaccg gcaaatttat cgatcgcctg ccactgtttg gtggtgataa ctccacccgg
     2761 cagacggcag cgcaacagca tcgcatggcg cggctccagc ttctgttcag cacgttcggc
     2821 gcggatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttatc
     2881 gcccttaaag ccgccggtca ggccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta
     2941 gttactttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc
     3001 tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca
     3061 cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga
     3121 agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca
     3181 ccgtcattga tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc ttgtacgtaa
     3241 actttttctt tttgatcgcg cgaccaggca agatcgatac gcgtcagcac gccctctttg
     3301 acgtaacgct gccactccac ctggtagagg aaatcttccg taaagtgcgg gttgccaaag
     3361 aacagccagt ttttacccgg cgcttcgtca gcggcgcgct gctgcataaa ggcgcggaac
     3421 ggcgcgatgc cggtgcctgg gccaatcata atcaccgggg tttccgggtt agcgggcagg
     3481 cggaagttat cgttatgttc gataaatacg cgaacttcgc cctcttcttc cacgcggtcc
     3541 gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca
     3601 ccaacggtga cgtgtacttc gttctcgact tcctcctgcg aggaggcgat ggaatacaga
     3661 cgcggcgtca gcgggcgcag cagattaatc agcgcttcgg cgtccagttg cgccggagag
     3721 aagcgcacca tgtcgacaat cggcgtagtc gcggcgtaat gctgtaactt cgctttatcg
     3781 cccaccagcg gcaacagcgt ttcgctgcgg gtaagcgtgg catagttctc aacaatattg
     3841 gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg
     3901 ccctcgacgg tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc
     3961 agtgccggat cgttctgata ccagacaccc agcgcgtcac ccggctggta acgcaggccc
     4021 gagtcaccta agtcaatttc gatatggcga acgtcttttt cagagttacg cccggtaatt
     4081 ttctggttaa ccgaaaggct cgctaccagc ggcgcgtctt tgctgtacgg gctggtgtgg
     4141 atttcattta ccacgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat
     4201 ttaagcgcat caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacgtcg
     4261 gcgtcgacac ggtcgagcag gcgttcaccg cccagttccg ccagcttgct gtcgaaatct
     4321 ttcccggact ggcagaaaaa ttcataagaa ctatcgccga ggctaaacac ggcaaaagcg
     4381 gtattttcca gcttcggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct
     4441 tccggcggtt ccccttctcc ttgcgttgac gtcaccacga taagcagttt ttcgctggcg
     4501 atttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt tgccgtcaat
     4561 aaatcatcgc gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga agcagagata
     4621 atagttatac ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc agcaggctgc
     4681 tgattgagca cgccccagaa atagccagaa acccaggcaa gctgggtggg agttaaatcg
     4741 gtcgtggccg cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt
     4801 gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt
     4861 cagcgcccat aaaacagaag agaaggtaag gttaacgggg tgaacggtgt ggattaaaga
     4921 cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa
     4981 caaaatagat taaccaacct aatgaaaaac aaatgaattt agccaatcat taagataaat
     5041 cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaagagcct ttccggtact
     5101 ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta tttaaagatt
     5161 tcaccttcga agccgctcac cgcttaccac acgtcccgga agggcataaa tgtggtcgcc
     5221 tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg
     5281 gctggattat cgatttcgct gaactaaaag cggcgtttaa gccaacttat gagcgcctcg
     5341 atcaccatta tctcaatgat attccaggtc tggaaaaccc aactagcgaa gttttagcaa
     5401 aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag
     5461 aaacctgcac cgctggttgt atctatcgcg gcgaatgata agagtgtggc ggcggtcaat
     5521 ttcccttaag taacgctatg ttagggtgtt gtgttcagga tatctggggc atgacatgga
     5581 agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg
     5641 ctgcgcgcga gcgggtttat ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa
     5701 aaatctttcc ggcgggcggt tatataccca tgcactcgcg gaactcctcc ctcaatttca
     5761 tctgaccgcg cctcttgaac gacgcatcac tcacgaaagc ctttccctgt taacgccgga
     5821 ttgcgcaacg acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc
     5881 acggttcgat ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccctgg
     5941 tgcgacggtg gatgcactgt atgaagaaaa cggcagggtg tgtggtgtca tttgtggtga
     6001 cgatattctc cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggctga
     6061 acgtcacggg ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt
     6121 gctgtcgctg gaaacatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc
     6181 agcgttgctg ttcagcggcg gaatctgtga tgacttaccc ggcggcgcat ttctttatac
     6241 taatcaacaa acgctctcgt tagggattgt ttgcccactc tcttccctta cgcaaagtcg
     6301 tgttccggca agcgagctgc ttgctcgctt taaaacgcat ccggcagtgc gcccgcttat
     6361 caaaaacacg gaatcactgg agtatggtgc gcatctggtg ccagaaggtg gcttgcacag
     6421 tatgccggta caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg
     6481 cgtcaatacc ggaatttctg tgcgcggcat ggatacggcg ctaactggcg cgcaggcggc
     6541 ggcacaaaca ctgataagcg cctgccagca ccgcgagccg caaaatctgt ttccgcttta
     6601 tcatcacaac gtagagcgca gcctgctgtg ggatgttcta cagcgttatc agcatgttcc
     6661 ggtgcttttg caacgcccgg gatggtaccg tacgtggcct gcgttaatgc aggatatttc
     6721 ccgcgattta tgggatcagg gtgataaacc tgttccaccg ctgcgccagt tactctggcg
     6781 tcatttacgt cgtcatggct tgtggaatct ggcgggcgat gttatcagga gtgttcgatg
     6841 tctgtagccc gtaatctctg gcgcgctgct gatgcgccgc acattgttcc ggctgactcc
     6901 gttgagcgtc agatggtaca acggttgatt aacgcctgtc cggtaggtct tttttcgctc
     6961 acaccggaag gtgacttacg cgttgattat cacggctgcc tggagtgtgg cacctgccgt
     7021 ttgctgtgcg acgaatccac actacaacag tggcgctatc cgccttccgg attcggcatc
     7081 acctaccgat ttggataaat aataaggata atttatgccc ctcttacacc tgctccgcca
     7141 gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcca tcgattctga
     7201 atgccagttt atttccgtct tgtacggcaa tatctgcacc attagtcaaa tcgttaaaaa
     7261 gattaagaac gctgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa
     7321 taaagaagtg gtgattcagt ttttgaaact ggtgaccgag gcggatggaa ttatcagcac
     7381 caaagcatcg atgctaaaag cggcaagagc ggaaggtttt ttctgtattc atcgcctgtt
     7441 tattgttgat tcgatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga
     7501 ttgtattgag atcctgccag gctgcatgcc caaagtgctg ggctgggtga cggaaaaaat
     7561 ccgccaaccg ctgattgctg gcgggctggt atgcgatgaa gaagatgcgc gtaatgcgat
     7621 taacgcgggt gtcgtggcgc tttccaccac gaataccgga gtctggacgt tagcgaaaaa
     7681 attgctttga cgggataatc gtaaccaatt gaatttggtt tgatttgtag gccgcacgcc
     7741 acatccgaca ttcagcgcct gatgcgacgc ttaacacgtc ttatcaggcc tacaggtccc
     7801 cagtccagaa ccgtaggcag gatcatccgg cagcacgtta ttggcattct gcgtcaatgt
     7861 ttgtcaccag tgcctccagc atcactttcc agtcatcaac cacgccaaca tctgcctgtg
     7921 aaaataccgc tgcgctggcg tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc
     7981 ccgccattaa cgctgctgcg ccagaagcgc caacaacaat acacacctca ggtgccagca
     8041 gatgtccgga aataccaatc actttttcag catcaacgcc accgttcatc actcgcgccc
     8101 gactgtagcc cacttcggct cccagctttt atgccagcat agcgatttcc tggttatctg
     8161 cctctccgcc ctgccccacc accaacaccc gtctggcctc agctaatggg tcgcgggtaa
     8221 catttttcag gttctctatg ctaaccagcc agtccggaag cgcacctggg actatgttca
     8281 gttgctgcat accagagggc aacgtggcat ttttatctgc tcccgcctgg cgcgccaggg
     8341 aaagacatag cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt ccccagtggg
     8401 atttgcgcac gcttacgatt ggaatctcta acgaagtcac ctggcagatg cttgcaccat
     8461 gtaaacgcca ggccagccga gtcgacagtt catcgccaaa cgttcccggc ggaaagagaa
     8521 caacgttcgc aggcgttcgc tgccactgtt ccaccagcgc atccagcacc tgttccacca
     8581 ctacgggctg tggttctatt tgccagtgcg ccagggtaca accgctgaaa tcctgcgccg
     8641 ccagccagcc cgctaatgcg gcattctcct gatttattgt cacaattgcg atattcatgg
     8701 ctgcatcctt tggcgtaaat agtcctgcca tagcttttgc gctttttctg ctaccgtttg
     8761 cccatcaatc agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca gacactgcat
     8821 cgctggcaat tcggcagcaa cagttttacg gataatttcc gctttccccg cagccattcg
     8881 ctggcgcatt cccggtactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc
     8941 aggcaagcga atccgacaac agcgcagccc atgttcagta cgctgttcga gggtgataaa
     9001 cggcgcgtcg agagtgaaac gctccacctg ggtgaagcaa ggccagccca gcatttccgc
     9061 cagcaaaaat ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag
     9121 atccagcgga ttctgacgct gccattcggc aatatggcga gcgacaaatt ccgacgcaaa
     9181 gcgcagatct gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata
     9241 gcgcagccag tgcaacgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt
     9301 accgtttttt ctctgcgcca acagcagcgc ggcggcagcc tgttcatcag caccgagtaa
     9361 acttcgcagt agcgaaatat ccggtccgct attaccatga gccgccgcct gccactcttt
     9421 ttccgccagc attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac
     9481 ctcctacatt tcccgccgcc accagcggga gtgctttggt ttctggtgcc catagccagg
     9541 ttacaacaaa accagccaac aacactgccg ccaggagcaa taaggtgact tgcattcccc
     9601 actgcgccag cacccacggc agcaggccag tacttaccgc cgcccccaac cgactcatgg
     9661 cggtggcaaa accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa
     9721 tgcccaccag attactgact gccgaaatgg tggtgctgaa gagaacaaaa gcagcagcgt
     9781 taatgaactg ccggaaggca aacaggccat cactaccagc gttgccgcca gcagcaaaaa
     9841 acttcccagc aaaaatttgc gatgtgccag caggtgcgtc agaactaatc ccagcagcgc
     9901 gcccacaatt aacaacgcat taagcatcag gctggcagtc agcgcatctt ccagtccaat
     9961 agtctgggcg atagttggca gccaggtata aatcacaaac catgggatta cgaggcagac
    10021 aaagaagacg ctgttaaacg ccgtgcgccg ccagtagcgc gaagagaaca aggttttgat
    10081 gtgtttatgg gtcgccgttg ccacttcatc gcccagtaaa acatggggac caaaatagcg
    10141 atgcacgata gcgtgagctt ctgcaaaacg cccctggcgc agtagccagc gtggcgattc
    10201 tggcgttccc cagcgtaata acgtaatcaa caacgcgggc agagctgccg atgccagcag
    10261 ccagcgccag gcctccgggt tttcggaaat aaagtgatgt ccggcaatac ttgccaacac
    10321 atagcctacg gtccacacca cgctgaatgc gcccagcaaa acaccgcgat ggcggcgcgg
    10381 ggaaaattca gccagcaagg tgtgacctac tgagtaatcg cctcccagac caatgccgat
    10441 caaaatgcgc agtccaataa gatgctctgg cgtggtggca aaaaattgca agaacgaagc
    10501 gagcgtaatc agcaaaaagc tgaaggtgaa gattttttgc cgaccaatat ggtcggagat
    10561 ccacccaaga accaggctgc caagaaacaa accaaggtgc gccgagccgc cgatcattcc
    10621 cgccataaac ggcgtcagtt gcatggcggg cgtaagttga ataatggcgt aaccaatgac
    10681 gccgagaaca taaccgtcgg tcaggtgtgc gccgaaagtg agcgcagcaa ttcgacagtg
    10741 aaaacggtta agcggtaaat catccattcg caccggtgaa gtgttcatct ttaccccttc
    10801 attgtccggt aataagaacg gcttctcgcc tgagaagccg aaaaggcaac gccccatcat
    10861 cgcggaacgc tgcctgacac attatttttc aatcggatag atagtacccg tattcataat
    10921 gccattagga tcgaactgct ttttcagccc ttccagcaac gcccacgcgc tgccgtgttc
    10981 cagcttgctc cagtgaacgc gatgtttacc gataccgtgg tggtgcacca tcgaaccacc
    11041 gaggcgaatg gtttcttcgc aaatgatttt gttgagcgga ttgtggtact tgtcgatttc
    11101 ctcttccggc ttacagttga caacgttgta gtcgtagacg aagtacatgt tggtgccgtt
    11161 ctgatagcta tgagaggagt gaccgcccag catggtgatg tcgtcggcgt gcgggaactc
    11221 ggtacgaata cggttaataa cgctttcgta gatttcgtgg atgcagctcc agcagccgga
    11281 cacttcggta gtaaagccca tgttgccggt tttgaggatc tgcacacgtt cggcagccac
    11341 tttatccggt ccccagttca ggttgttaaa ccaggtttcg atcagcttgc tgtccacgcg
    11401 ttggcattgc gggtagcggg caacgatttc cgcaatccct tcgcccgtcg ccttcgcaat
    11461 gcgagggtta ccttcagcca taaagatcag cacgcatttt ccgtcggcaa aatgggtgaa
    11521 gtgttgggtg ccatcttcag cgtcatacaa acgagcgatc gacggacgat acccttccac
    11581 catgatttca cgcaggatgt tgaagccggt tttcatgtct tccaggatat agccgtagaa
    11641 gaggttgttt tccggggtga atttaaagat tttcactgtt acttcagtga tatagcacaa
    11701 tgcaccttcg ttgccgatga tgatgtgacg aatgtccggg ccagccgcgc ggcgtggcac
    11761 gtttttaatg cgtgtgacgg tgccatctgc caacactgcc tccagaccaa cgaccatatc
    11821 ttcgattgcg ccgtagagtg tggagaactg cccgatactg cgggttgcta ccaggccgcc
    11881 catctgcgcc agcggctttg actgcggaga atgccccgtg gtgtaacctt tttcacgcaa
    11941 cgcgttttcc agcacctcca gcggaacacc acattgcgcc gtcgcctgca tattctcaat
    12001 atcaatatta atgatttgat tcatggcgga accgtcgagc accaccgagt tttctacaac
    12061 agtttccagc ccaccttcgg tggcggaagc accggtacgc ggcacaccgt taattttgtg
    12121 cgcattcata aaattcagca cacgggatac ttgctctgtg gaaccgagtt ttacgaccgc
    12181 tgccggaatc ggcaaagtat aaatgccatg aatatccgga aatttacgaa aacggtcaat
    12241 actgttcttc tttaatactg tttcatcggt aattacgcga tctgcaccaa caatttcctt
    12301 tagctggtcg acaatcgctg cgcgagataa agacatagta attccttcct gataataaaa
    12361 atggaataat cattaataag agaaaacaga aaccaccgtt atgggttagc gcactaaata
    12421 accgccatcg accactaata aatgcccgtt gacataattc gatgcctgac ttgcgagaaa
    12481 cacggctgcg cccattaaat cctgagtatc gccccaacgg tttgccggaa tatgatcaag
    12541 aacgcgctga ttggtttctg gattactgcg tgtcgccagc gtaatgtctg ttgcataata
    12601 gccaggggcg ataccattta cctgaatatt atattgacct agttcatcac aataagcttt
    12661 ggtgaatccg gcaagagcat gtttagtagc agaatatgca ggtgaccatt gtccacctaa
    12721 gtaagagaac aatgaacaga tattaatgat tttaccgctt ttctgcggga tcataatttt
    12781 tgccgcttcg tagcttaact cgaatgcggc ggtcaggttc acatcaatca tcgggtccca
    12841 gtcggcacga ccgaagtcca gcaccttatt cagcttacaa ataccggcat tgttaaccag
    12901 aatatcaact gtaccgaaac gctcacagca ggcagcgata atcttctgcg gcgcgccttc
    12961 tgcggtgata tccacctgca tgaagtccac ctcaacaccc tgattttcaa tcatttcctt
    13021 tgtttcgccg ttatctttga caaaacttgg aataaagaca tttgcgcctg ctttggccaa
    13081 cgccatggca aatgcctggc ctaaaccgct attcccgccg gtaacaattg cggttttacc
    13141 tttcagggag aaaaaatcca ttgagaacgc attgagagat tcgattgaca tagttagctc
    13201 caatttccgt aaggcaaaaa aaaagaaagg taacttcccc cgcgagaggg aagttacctt
    13261 tctcattatc tctggtaact ggagataatt tttagcataa ggacataact gctaatgtga
    13321 tcgtaatcac agtgtgatat tcgtattatc aaatagtttt aaaaagcaga agcgtgatta
    13381 ctctcacaca acacctctgc tttccatgtt atttattatt tcaattacta gagacct
//
LOCUS       DAZKIF010000121        13272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZKIF010000121 DAZKIF010000000
VERSION     DAZKIF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.122"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            383..1096
                     /gene="aphA"
                     /locus_tag="ACHOTW_003715"
     CDS             383..1096
                     /gene="aphA"
                     /locus_tag="ACHOTW_003715"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP9845747.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1207..1623
                     /locus_tag="ACHOTW_003716"
     CDS             1207..1623
                     /locus_tag="ACHOTW_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP9845748.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1627..1983
                     /locus_tag="ACHOTW_003717"
     CDS             1627..1983
                     /locus_tag="ACHOTW_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9845749.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2018..4840)
                     /gene="uvrA"
                     /locus_tag="ACHOTW_003718"
     CDS             complement(2018..4840)
                     /gene="uvrA"
                     /locus_tag="ACHOTW_003718"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9845750.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5095..5631
                     /gene="ssb1"
                     /locus_tag="ACHOTW_003719"
     CDS             5095..5631
                     /gene="ssb1"
                     /locus_tag="ACHOTW_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP9845751.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5730..6011)
                     /locus_tag="ACHOTW_003720"
     CDS             complement(5730..6011)
                     /locus_tag="ACHOTW_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP9845752.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6440..8026
                     /gene="pdeC"
                     /locus_tag="ACHOTW_003721"
     CDS             6440..8026
                     /gene="pdeC"
                     /locus_tag="ACHOTW_003721"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP9845753.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(8029..8352)
                     /gene="soxS"
                     /locus_tag="ACHOTW_003722"
     CDS             complement(8029..8352)
                     /gene="soxS"
                     /locus_tag="ACHOTW_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP9845754.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8438..8902
                     /gene="soxR"
                     /locus_tag="ACHOTW_003723"
     CDS             8438..8902
                     /gene="soxR"
                     /locus_tag="ACHOTW_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP9845755.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9448..10797
                     /gene="ghxP"
                     /locus_tag="ACHOTW_003724"
                     /gene_synonym="yjcD"
     CDS             9448..10797
                     /gene="ghxP"
                     /locus_tag="ACHOTW_003724"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP9845756.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10948..12597
                     /locus_tag="ACHOTW_003725"
     CDS             10948..12597
                     /locus_tag="ACHOTW_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP9845757.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12751..>13272)
                     /gene="espX5"
                     /locus_tag="ACHOTW_003726"
     CDS             complement(12751..>13272)
                     /gene="espX5"
                     /locus_tag="ACHOTW_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFP9845758.1"
                     /translation="NLYKVIAPYINLFRADLSFSKLDLINFKHADLSRVNLNKAILQN
                     INLIDSKLFFTRLTNTFLEMVICTDSNMANVNFNNANLNNCHFNCSVLTKAWMFNTRL
                     YRVNFDEASVQGMGISILRGEENIPINSDTLVTLQKFFEEDCTSHTGMSQTENNTHEV
                     AMKITADIMQHAD"
BASE COUNT         3119 a         3234 c         3459 g         3460 t
ORIGIN      
        1 agtaataaaa ttaatcacca ttgtagggta gggttctggt caatcagaaa tcatctttat
       61 aaacttcgat tgtttttgta atgctgtatc attaagttca ttaaatcgta cagcagataa
      121 atgttctatc aaatttcgct catttgcaga gtattcatca taataaacgt aaaattaatg
      181 tatccttaca tcgagcatta accatttttt acaaaaaaac gacaggaacg tatttactag
      241 gttaaatata atcatcctgc ttttcatcac aaaaaccgca gataatcctt cctttcccct
      301 gtagctggcg ttatggtcag atagtttttg caacaaatct cacaataaaa agtttcaaca
      361 tactgactat ttagggaaaa atatgcgcaa gatcacacag gcaatcagtg ccgtttgctt
      421 attgttcgct ctaaacagtt ccgctgttgc cctggcctca tctccttcac cgcttaaccc
      481 tgggactaac gttgccaggc ttgctgaaca ggcaccaatt cattgggttt cggtcgcaca
      541 aattgaaaat agcctcgcag ggcgtccgcc aatggcggtg gggtttgata tcgatgacac
      601 ggtacttttt tccagtccgg gcttctggcg cggcaaaaaa aacttctcgc cagaaagcga
      661 agattatctg aaaaaccctg tattctggga aaaaatgaac aacggctggg atgaattcag
      721 cattccaaaa gaggtcgctc gccagctgat tgatatgcat gtacgccgtg gtgacgcgat
      781 cttctttgtg actggtcgta gcccgacgaa aacagaaacg gtttcaaaaa cgctggcgga
      841 taattttcat attcctgcca ccagcatgaa tccggtgatc tttgcgggcg ataaaccagg
      901 gcaaaataca aaatcgcaat ggctgcagga taaaaatatc cgaatttttt atggcgattc
      961 cgataacgat attaccgccg cacgcgatgt cggcgctcgt ggtatccgca ttctgcgtgc
     1021 ctccaactct acctacaaac ccttgccaca agcgggtgcg tttggtgaag aggtgatcgt
     1081 caattcagaa tactgacaga gcgggagagc gtgacactct cccgaatgct gtttttttaa
     1141 tcacaccttt atcctttcgc tgtcttgctg caaactgatt aagagagttt tatcaaggag
     1201 cagcacatgt ggtatcaaaa gacgctcacg cttagcgcca aatctcgtgg gtttcatctg
     1261 gtaacggatg aaattctgaa tcagctggct gatatgccgc gcgttaacat cggcttactg
     1321 catctgttgc tgcaacatac ctccgcctct ctgacactta atgagaactg cgatcccacc
     1381 gtacgccacg acatggagcg ttttttcctc cgcaccgttc ccgacaacgg aaattatgag
     1441 catgactatg agggagcaga cgatatgcct tctcatatca aatcctcaat gctgggaaca
     1501 tcgcttgtat tgccggtgca taaagggcgt attcagaccg gcacctggca aggcatttgg
     1561 ctgggggaac atcgcatcca cggcggatcg cgtcgcatca tcgcgacact acaaggggag
     1621 taaaaaatga ccatttcgga gttgctacaa tattgcatgg caaaaccagg cgcagaacag
     1681 agcgtgcata acgactggaa agcgacgcag atcaaagtgg aagatgtact gtttgcgatg
     1741 gtgaaagaag tagaaaatcg cccagctgtt tcgctgaaaa ccagcccgga gctggcggag
     1801 ctgctacgtc agcagcacag cgatgtgcgt ccaagccgcc atctgaataa agcgcactgg
     1861 agcaccgtgt atctcgacgg ttcgctgccg gattcgcaaa tctattatct ggtggatgcg
     1921 tcttatcagc aggcggtgaa tttactgccg gaagaaaaac gtaaattgct ggtgcaactc
     1981 tgaaaggaaa aggccgctcg gaaggcggcc ttaacgatta cagcatcggc ttgaggaagc
     2041 gtgccgtatg cgaagcttcg cactccgcga cggtttctgg cgtaccggag acgaggatct
     2101 cgccgccgcc actgccgcct tccggcccca ggtcgacaat ccagtcagcg gttttaatca
     2161 cgtcgagatt gtgctcaatc accacaatgg tgttgccctg atcgcgcagt ttatgcagta
     2221 cgtcgagcag ttgctgaata tcggcgaagt gcagaccggt ggtcggctcg tcgagaatat
     2281 acagcgtctg cccggtgccg cgttttgaca gctcacgcgc cagcttcacg cgctgggctt
     2341 caccaccaga aagtgtggtt gcggactgcc ccaagcgaat gtacgtcaga ccaacgtcca
     2401 tcaacgtttg cagcttacgc gccagagctg gcaccgcatc aaagaactca cgcgcctctt
     2461 cgatggtcat atccagcact tcgtggatgg ttttgccttt gtacttaatc tccagcgttt
     2521 cacggttata gcgtttacct ttgcactggt cgcacggcac gtaaatgtcc ggcaggaagt
     2581 gcatctccac tttgatcacg ccatcgccct gacaggcctc gcagcgtccg ccacggacgt
     2641 taaagctgaa acgtcctggc gtataaccac gcgcacggga ttccggtacg cccgcaaaca
     2701 gttcgcgcac aggcgtaaac acgccggtat aggtcgccgg gttagaacgc ggagtacgac
     2761 caattgggct ttggtcgata tcgattactt tgtcgaaatg ctccagtccc tgaatatcgc
     2821 gatacggtgc cggttcggcg atggtcgcac cattcaactg gcgttgggca atcgggaaca
     2881 gtgtgtcgtt aatcagcgtc gatttaccgg aacctgaaac ccctgtgatg caggtaaaca
     2941 gaccgactgg cagcgttagc gtcacgtctt tcaggttgtt gccgcgtgcg ccagtcagct
     3001 taagtacttt ttccggattc gccggaacgc gtttcttcgg cacttcaatc ttgcgtttac
     3061 cgctcatgta ctgtccggtc aacgactccg gcaccgccat aatcgcttcc agcggacctt
     3121 ccgcgaccac ttcaccgcca tgtacacctg caccaggacc gatatcgatc acatgatcag
     3181 cggcgcgaat cgcgtcttcg tcgtgctcca ccacaatcac ggtattaccg agatcgcgca
     3241 gatggataag cgtacccaac aggcgctcgt tatcgcgctg gtgcaggccg atagacggct
     3301 catccagcac gtacataacg ccaaccaggc ccgcaccaat ctggctcgcc agacggatac
     3361 gctgggcttc accgccggaa agcgtttctg ccgagcggga aagcgtcagg taattcaggc
     3421 cgacgttaac gaggaatttc aaacgatcgc cgatctcttt aagaattttt tccgcaatct
     3481 tcgcccgctg acctgcgagt ttgagattgt tgaagaattc catcgcatga ccaatgctca
     3541 tgtcggagat ggcgggcagc ggcgtattct cgacatacac gtggcgcgct tcccgacgca
     3601 gacgcgtccc ttcgcagctg gcgcacgggc gattgctgat gaacttggct aattcttcac
     3661 gtaccgcact ggattctgtc tctttataac ggcgctccat attgtgcagc acgccttcga
     3721 acggatgacg acggatggag gtatcgccac gatcgttcat gtatttgaat tcaatgtttt
     3781 ctttgccaga accgtacaac accactttat gcacgttcgc gctcaggctg ccccacggcg
     3841 cttcgacgtc gaacttatag tgatctgcca gcgatttcag catctggaaa taatagaagt
     3901 tgcggcgatc ccagccacgg atcgcaccac ctgccagcga cagttccgga ttctggatca
     3961 cgcggtcagg atcgaaatat tgctgtacgc caaggccgtc gcaggtcggg caggcacctg
     4021 ccgggttgtt aaacgaaaac agtcgcggct ccagttcacg catactgtag ccgcaaattg
     4081 ggcaggcgaa gttggcggaa aacagcagct cttccgcttt cgggtcgtcc atatccgcca
     4141 ctaccgcggt accaccggaa agctccagcg cggtttcaaa cgactcggca agacgttggg
     4201 taagatcgtc acgcaccttg aagcgatcaa ccaccacttc aatagtgtgt ttcttttgca
     4261 gttccagttt cggcggatcg gaaagatcgc agacttcgcc atcaatacga gcacggatgt
     4321 aaccctggct tgccaggttc tccagcgttt tggtgtgttc gcctttgcgc tctttaatga
     4381 ttggcgcgag cagcatcaga cgcttgcctt ccggctgcga cagcacgtta tccaccatct
     4441 ggctgacggt ttgcgccgcc agcgggacgt cgtggtccgg acagcgcggt tcgccgacgc
     4501 gggcgtacaa caaacgcaaa tagtcgtgga tttcggtgat tgtccccacc gtagaacgcg
     4561 ggttatgaga cgtcgatttc tgctcaattg agatggcagg agaaagcccc tcaatatgat
     4621 cgacgtccgg cttttccatc agtgacagaa actgccgcgc gtaggcggaa agggattcaa
     4681 cgtaacggcg ctgcccttcg gcatataagg tgtcgaaagc gagcgaggat ttgccagaac
     4741 ccgaaagccc ggtcacgaca atgagcttgt cgcgggggat aacgaggttg atgtttttga
     4801 gattatgggt gcgggcgccc cgaacttcga tcttatccat tcacctttcc cggattaaac
     4861 gcttttttgc ccggtggcat ggtgctaccg gcgatcacaa acggttaatt atgacacaaa
     4921 ttgacctgaa tgaatataca gtattggaat gcattacccg gagtgttgtg taacaatgtc
     4981 tggccaggtt tgtttcccgg aaccgaggtc acaacatagt aaaagcgcta ttggtaatgg
     5041 tacaatcgcg cgtttacact tattcagaac gatttttttt caggagacac gaacatggcc
     5101 agcagaggcg taaacaaggt tattctcgtt ggtaatctgg gtcaggaccc ggaagtacgc
     5161 tacatgccaa atggtggcgc agttgccaac attacgctgg ctacttccga atcctggcgt
     5221 gataaagcga ccggcgagat gaaagagcag actgaatggc accgcgttgt gctgttcggc
     5281 aaactggcag aagtggccag cgaatatctg cgtaaaggtt ctcaggttta tatcgaaggt
     5341 cagctgcgta cccgtaaatg gaccgatcaa tccggtcagg atcgctacac cacagaagtc
     5401 gtggtgaacg ttggcggcac catgcagatg ctgggtggtc gtcagggtgg tggcgctcct
     5461 gcaggtggca atatcggtgg tggtcagccg cagggcggtt ggggtcagcc tcagcagccg
     5521 cagggtggca atcagttcag cggcggcgcg cagtctcgcc cgcagcagtc cgctccggca
     5581 gcgccgtcta acgagccgcc gatggacttt gatgacgata ttccgttctg atttgtcatt
     5641 aaaacaatag gttatattgt tttaaggtgg atgattaaag catctgccag ccataaaaaa
     5701 gaagcctccg ttatggaggc ttctacgtat caggtcaaaa tcattggcca ctgtggcggt
     5761 gtctgattca tctcttctat catcaccgac tcgatgttgt gccagatggc ggtgatgtcc
     5821 gccattgtga tgccaagcaa ccccattgcg aaccagcagg cggaaaccac gcccagaccg
     5881 ctgctgatca ctgcaagccc gacataatca gaccgacgaa aacggatgtt gagcgtgctg
     5941 gccagaaaca tcattacggc actaagaagt tgccagcgaa gaagaaccac gccagtggtg
     6001 agggtagcca tcaaacaatt cctctgaaaa gagccgatgc cctggacagc gcggcgttgt
     6061 tcacgggagg cgtggtacac tctggctatc gcggggcttg cagaacacaa aaatgaaaca
     6121 ctctgtttgt ttcattaatt ttgtgaacta tatcacaatt gattgtttgt tagccagatt
     6181 aggtcgtgac ttttattgct gtacagatta tgtggttttt cagtggcatt aagggtatat
     6241 cttcccgccg cctctgtatt cctgtagaaa attaattttg aatatcaatg aattattttc
     6301 atccaagtga cgattaaaaa ggtatcaatt tcaaatcagg caaaagtgct atttataccg
     6361 taagatttat ctaaagacgt cggtagccag ggtttcacct tgcaatggcc gggtataaac
     6421 aggcaggaaa ttgatagcaa tgaatcatcg tgcacggcac caattactgg cgttgccggg
     6481 cattatcttt ttagttctct ttcccatcat tctttcgcta tggattgcct tcctttgggc
     6541 aaaatcagaa gtgaataatc agctccgaac ctttgctcaa ctggcgttgg ataaatccga
     6601 gctggtcatt cgccaggcag atttagtgag cgatgcagct gaacgctatc aggggcaagt
     6661 ttgcactcca gcccatcaaa agcgaatgtt gaatattatt cgtggctatc tttatattaa
     6721 tgaattgatc tatgcccgtg ataatcattt tttatgctca tcgctgatag tgcctgtaaa
     6781 cggctatacg attgtatcgg ccgattataa gcgtgaacct aatgtttcta tctattatta
     6841 ccgcgatacg ccttttttct ctggctataa aatgacctat atgcagcggg gaaattatgt
     6901 ggcggttatc aaccctctct tctggagtga agtgatgtct gatgacccga cattgcaatg
     6961 gggtgtgtat gatacggtga cgaaaacctt tttctcgtta agcaaagagg cctcagcagc
     7021 aacgttttct ccgctgattc atttgaagga tttaaccgta ccaagaaatg gctatttata
     7081 tgcgacagtt tattcgacaa aacgcccaat tgcagccatt gttgcgactt catatcaacg
     7141 tcttatagcc catttttata atcatcttat ttttgcgttg cccgccggta ttttggggag
     7201 tcttgttctg ctattactct ggctacgtat tcgacaaaac tatttgtctc ccaaacgcaa
     7261 attgcaacgc gccctcgaaa aacatcagct atgcctttat taccagccaa taattgatat
     7321 caaaacagaa aaatgtatcg gcgctgaagc gttgttacgt tggcctggtg agcaggggca
     7381 agtaatgaat ccggcagagt ttattcctct ggcagaaaag gaggggatga tagaacaggt
     7441 aactgattat gttattgata atgtcttccg cgatctgggc gcgtaccttg caacacatgc
     7501 tgatcgctat gtttctatta atctgtcggc ctccgatttt catacgtcac ggttgatagc
     7561 gcgaatcaat cagaaaacag agcaatacgc ggtgcgtccg cagcaaatta aatttgaagt
     7621 gactgagcat gcatttcttg atgtcgacaa aatgacgccg attattctgg ctttccgcca
     7681 ggctggttac gaagtggcaa ttgatgattt tggtattggc tactctaact tgcataacct
     7741 taaatcattg aatgtcgata ttttgaaaat cgacaaatcg tttgttgaga cgctgaccac
     7801 ccataaaacc agtcatttga ttgctgaaca tatcatcgag ctggcgcaca gcctggggtt
     7861 aaaaacgatc gctgaaggcg tcgaaactga ggagcaggtt aactggctgc gcaaacgcgg
     7921 cgtgcgctat tgccagggat ggttctttgc gaaggcgatg ccgccgcagg tgtttatgca
     7981 atggatggag caattacccg cgcgggagtt aacgcgcggg caataaaact acaggcggtg
     8041 gcgataatcg ctgggagtgc gatcaaactg ccgacggaaa acgcgggaga aggtctgctg
     8101 agagacataa cccaggtcca ttgcgatatc aaaaatcgga cgctcggtgg tgcgcaactc
     8161 aacggcggcc agtaacagac ggcgttgacg aatgtaatcg ccaagcgtct gatgcgtcac
     8221 cgtgcggaac atccgttgca ggtaccactt tgaatagcct gatttttttg cgacgacatc
     8281 aatgttaagc ggctggtcaa tatgctcgtc aatccatgcg ataagatcct gaataatttt
     8341 ctgatgggac ataaatctgc ctcttttcag tgttcagttc gttaattcat ctgttgggga
     8401 gtataattcc tcaagttaac ttgaggtaaa gcgatttatg gaaaagaaat taccccgcat
     8461 taaagcgctg ctaacccccg gcgaagtggc gaaacgcagc ggtgtggcgg tatcggcgct
     8521 gcatttctat gaaagcaaag ggttgattac cagtatccgt aacagcggca atcagcggcg
     8581 ttataaacgt gatgtgttgc gatatgtcgc aattatcaaa attgctcagc gtattggcat
     8641 tcctctcgcg accattggtg aagcgtttgg cgtgttgccc gaagggcata cgttaagtgc
     8701 gaaagagtgg aaacagcttt cgtcccaatg gcgagaagag ttggatcggc gcattcatac
     8761 cttagtggcg ctgcgtgacg aactggacgg atgtattggt tgtggctgcc tttcgcgcag
     8821 tgattgcccg ttgcgtaacc cgggcgaccg cttaggagaa gaaggtaccg gcgcacgctt
     8881 gctggaagat gaacaaaact aaagcgccac aagggcgctt tagtttgttt tccggtcttt
     8941 gtctttctct ctatcccgct ggtacacagg agggtttccc ccgacgtcaa cacacctcat
     9001 tcgagcacgt ggtggaggtt ccggttggtg ttgatgcttt aattgtatgt caccgacgtt
     9061 tcttcgccag tgtaaaagta tactttttag ccgcaatatt tttgtcatct cagacgattt
     9121 tttatcgcaa tcctcaacgg tatacggctc gataacgctg caatcttgcg catcgacgat
     9181 aacgtttgcg catcaattgc ctggtttttc atcgtcaaga caataaaaga gaaaaaagca
     9241 gcaaacttcg gttgaaaaag ccgctatgat cgccggataa tcgtttgctt tttttaccac
     9301 ccgttttgta tgcgcggagc taaacgtttg cttttttgcg acgcagcaaa ttgtcgcaaa
     9361 cctggagcag gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa
     9421 aattaaactc tcaggggatg ttctcttatg tctacgccat cagcgcgtac cggcggttca
     9481 ctcgacgcct ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc
     9541 gggttaacaa cgtttctggc gatggtctac tcggttatcg tcgttccagg catgttgggt
     9601 aaagcgggct tcccgcctgc ggcagttttc gttgcgacct gtctggttgc cggactcggt
     9661 tctatcgtga tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg
     9721 acggcgttta ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg
     9781 ggtgccgtgt tcctgatggg tgtgctgttt acggtaattt ctgccacggg tatccgtagc
     9841 tggattttgc gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg
     9901 ttcctgctgc tcattgccgc taacggtgtc ggtctggtga ttaaaaaccc gcttgatggt
     9961 ctgcccgttg cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg
    10021 gcggtgatca tcggcctgga aaaactgaaa gtccctggtg gcattctgct gaccattatc
    10081 ggtatctcaa ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc
    10141 atgccttcat tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc
    10201 gcgctgaatc ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat
    10261 gccaccggaa ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag
    10321 atcatcgacg gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcttg
    10381 gtgggtgcag ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt
    10441 aaaaccggtt tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct
    10501 ccgctttctt acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg
    10561 ctgatgctga gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt
    10621 ctggttacgg cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc
    10681 ttcgcgactc tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt
    10741 acggtcgtta tcgccgtggc gctggtgacc ttctatgcgg gtggctgggc tatctaatct
    10801 ttcatcggat tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg
    10861 tgtgagcttt tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc
    10921 ataaaaaaca tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc
    10981 ctcgtggtct cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg
    11041 cttatgcaaa tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag
    11101 tttgatcctg aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg
    11161 aaaacgccga cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg
    11221 ctggtggtgg tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt
    11281 ccgctgatcc ccgcctttgc gctggcggcg gtgctttctc cgaccgatgc tgtggcgctc
    11341 tccgggattg ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa
    11401 gcgttgatga atgacgcctc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg
    11461 gggacgatga tcttcaccgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc
    11521 ggtattctcg ccggttttgt ggtgagctgg ctgtacggtc gctcgctgcg attcctcagc
    11581 cgctggggcg gtgatgaacc cgcgacgcag atcgtcctgc tgttcctgct gccattcgct
    11641 tcttatctga ttgccgaaca tattggcgtt tccggcatcc tcgctgcggt tgccgccggg
    11701 atgaccatca cccgctccgg tgtgatgcgc cgtgcgccgc tggcaatgcg cctgcgtgca
    11761 aacagcacct gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt
    11821 ctgcagctgc cgggtattct ggagacgtcg ctgatggcag cagaaatcga ccctaacgtc
    11881 gaaatctgga tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt
    11941 ttcggctggc tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg
    12001 gagtttggtt cgtggaccac acgagaaatc ctgatcgcgt ctttcgccgg ggtgcgtggg
    12061 gcgatcactt tggccggtgt gctctctatc ccgctgctct tgccggatgg taacgtcttc
    12121 ccggcgcgct atgagctggt gtttctggcg gctggtgtca ttctcttctc gctgtttgtc
    12181 ggcgtggtga tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa
    12241 ttgaaagaag aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag
    12301 aaaatggagg agcgtctggc ggcggatacc gaagagaata tcgataacca gctgcttacg
    12361 gaggtcagtt ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt
    12421 gaaagttccg tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct
    12481 gaacgtgctg aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa
    12541 aaattactgc acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc
    12601 gaaagcgacg taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa
    12661 caggttagct cgggctaaca ttcatgattc taaaacaaat aagttatctc tcttttaaag
    12721 tcatttcatt ggctgttctt cgacttaatc tcaatctgcg tgttgcataa tatctgcagt
    12781 aatcttcata gctacttcat gagtattatt ctcagtttgt gacatgccag tatgagaggt
    12841 acaatcttct tcaaagaatt tctgtagtgt taccagggta tcactattaa tgggaatatt
    12901 ctcctcccca cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac
    12961 ccgatagaga cgggtattaa acatccaggc ttttgttaaa acagaacagt tgaaatggca
    13021 attgtttaaa ttggcattat taaaattaac attagccata ttagaatcgg tgcatataac
    13081 catttcgagg aacgtatttg ttagccgtgt aaaaaagagt ttactatcaa ttaagtttat
    13141 attctggagg attgctttgt tcagattgac gcgagacaga tcagcatgtt taaagttaat
    13201 taaatcaagt ttagagaagc taaggtcggc tctaaataaa ttaatatatg gcgctattac
    13261 tttataaaga tt
//
LOCUS       DAZKIF010000122        13208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZKIF010000122 DAZKIF010000000
VERSION     DAZKIF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.123"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            86..499
                     /gene="hns"
                     /locus_tag="ACHOTW_003727"
     CDS             86..499
                     /gene="hns"
                     /locus_tag="ACHOTW_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP9845759.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(643..1551)
                     /gene="galU"
                     /locus_tag="ACHOTW_003728"
     CDS             complement(643..1551)
                     /gene="galU"
                     /locus_tag="ACHOTW_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9845760.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1753..2766)
                     /gene="rssB"
                     /locus_tag="ACHOTW_003729"
     CDS             complement(1753..2766)
                     /gene="rssB"
                     /locus_tag="ACHOTW_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP9845761.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2858..3763)
                     /gene="rssA"
                     /locus_tag="ACHOTW_003730"
     CDS             complement(2858..3763)
                     /gene="rssA"
                     /locus_tag="ACHOTW_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP9845762.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3876..4334
                     /gene="ychJ"
                     /locus_tag="ACHOTW_003731"
     CDS             3876..4334
                     /gene="ychJ"
                     /locus_tag="ACHOTW_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP9845763.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            4384..5226
                     /gene="purU"
                     /locus_tag="ACHOTW_003732"
     CDS             4384..5226
                     /gene="purU"
                     /locus_tag="ACHOTW_003732"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP9845764.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            5386..5470
                     /locus_tag="ACHOTW_003733"
     tRNA            5386..5470
                     /locus_tag="ACHOTW_003733"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5420..5422,aa:Tyr,seq:gta)
     gene            5515..5645
                     /locus_tag="ACHOTW_003734"
     ncRNA           5515..5645
                     /ncRNA_class="other"
                     /locus_tag="ACHOTW_003734"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            5680..5764
                     /locus_tag="ACHOTW_003735"
     tRNA            5680..5764
                     /locus_tag="ACHOTW_003735"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5714..5716,aa:Tyr,seq:gta)
     gene            5811..5942
                     /locus_tag="ACHOTW_003736"
     ncRNA           5811..5942
                     /ncRNA_class="other"
                     /locus_tag="ACHOTW_003736"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            5989..6120
                     /locus_tag="ACHOTW_003737"
     ncRNA           5989..6120
                     /ncRNA_class="other"
                     /locus_tag="ACHOTW_003737"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6304..6981)
                     /gene="narI"
                     /locus_tag="ACHOTW_003738"
     CDS             complement(6304..6981)
                     /gene="narI"
                     /locus_tag="ACHOTW_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9845765.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(6981..7691)
                     /gene="narJ"
                     /locus_tag="ACHOTW_003739"
     CDS             complement(6981..7691)
                     /gene="narJ"
                     /locus_tag="ACHOTW_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9845766.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(7688..9226)
                     /gene="narH"
                     /locus_tag="ACHOTW_003740"
     CDS             complement(7688..9226)
                     /gene="narH"
                     /locus_tag="ACHOTW_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9845767.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(9223..12966)
                     /gene="narG"
                     /locus_tag="ACHOTW_003741"
     CDS             complement(9223..12966)
                     /gene="narG"
                     /locus_tag="ACHOTW_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP9845768.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3204 a         3550 c         3239 g         3215 t
ORIGIN      
        1 tacattcctg gctattgcac aactgaattt aaggctctat tattacctca acaaaccacc
       61 ccaatataag tttgagatta ctacaatgag cgaagcactt aaaattctga acaacatccg
      121 tactcttcgt gcgcaggcaa gagaatgtac acttgaaacg ctggaagaaa tgctggaaaa
      181 attagaagtt gttgttaacg aacgtcgcga agaagaaagc gcggctgctg ctgaagttga
      241 agagcgcact cgtaaactgc agcaatatcg cgaaatgctg atcgctgacg gtattgaccc
      301 gaacgagctg ctgaatagcc ttgccgccgt taaatctggc accaaagcta aacgtgctca
      361 gcgtccggca aaatatagct acgttgacga aaacggcgaa actaaaacct ggactggcca
      421 gggccgtact ccagctgtaa tcaaaaaagc aatggatgag caaggtaaat ccctcgacga
      481 tttcctgatc aagcaataat cttttgtaga ttgcacttgc ttaaaatccc gccatcggcg
      541 ggatttttta ttgtccggtt taagacaatt taataagaat aagctataaa aaaacggcgt
      601 cgattgctca acgccgtttc gtggataaca ccgatacgga tgttacttct taatgcccat
      661 ctcttcttca agccaggctt taaattccgt gccaagggtg ttatgacgaa taccgtattc
      721 aacgaaggcc tgcatgtaac ctaatttatt accgcagtca tggctcttcc ctttcatatg
      781 ataggcttca accgtttctt tttcgattag catatcaatt gcgtcggtga gctgaatttc
      841 atcaccagct cccggagggg tttttgccag caacggccaa atatccgcac taagtacgta
      901 acgacccaca atagcgagat tagacggcgc aacatccgct tttggttttt caaccacgcc
      961 aaccatcggt acgctttcac ccggcgctaa ttcaacgcct ttgcaatcca caacgccata
     1021 tgcggtcaca tcagcaaccg gttcaaccat gatctggcta tgacccgttt catcgaagcg
     1081 gcggatcatc tctgccaggt tatcctgtga caaatcggat tcatattcat ccagaataac
     1141 gtcaggcaaa ataacagcca caggttcatc acccactacc gggtgagcac acaataccgc
     1201 gtgtcccagg cctttcgcca gcccctgacg aacttgcata atagttacgt gcggcggaca
     1261 aatagactgc acttcatcaa gcagttgacg ttttacacgt ttttccagca ttgcttccag
     1321 ttcaaaactg gtatcaaagt ggttttcaat agagttttta gatgagtgtg taaccagcac
     1381 aatttcagta atgccagccg caatacattc attcacgacg tattgaatta atggcttatc
     1441 gacaagtggc agcatctctt tcgggatggc tttcgtcgcc ggcaacatcc tggttcctaa
     1501 tcccgcaacg gggataacgg cttttttgac tttcgtatta atggcagcca tttaaattct
     1561 cctggactgt tcgtgttttg aacgtgttca tatttctgta tcgcatccca gtatatcagc
     1621 accccggagg ggctaagttc tgaaaaggat gcgagttagc ataattaaga caattactta
     1681 tacgaatggc gacgatagta ccaccagccg acattagctg gtaatgcaaa tttagcccgc
     1741 gttatcgttt gctcattctg cagacaacat caagcgcagt cgaccaccgg ttccccatat
     1801 ttggcattgc caggcatcgc atcgctggct caattgattc aaataagcgt tacctaaagt
     1861 gcctaacgga acaccattac tgatttgcac ctggtgttcg ccggtattta acgtcgcatt
     1921 cagacccgca gaaaccagaa tgagattttt cagttcgcga tgataatagc caactaatag
     1981 cggaaactgc cccggcagat tggcctgacg gagtaaatgg tttacctgct tcaataacgc
     2041 gcccaactct ggcaaccgtt gattttggtg tgcaagctgt tcctgtaata atccgttaaa
     2101 caatgcgcgt aataacaagg cagcaagtac gccattatgt ccagctcggg ttacatcaag
     2161 gcaataaaat gccagatcgt tttccgaaag tgcggcaata tcaagcacca ggccgggttt
     2221 gtccgcggca accaattgac ggtaattaac ccggcaatgg gaaatcacct gctgaaccgg
     2281 cggttgtagt tcctgtaata atttcgccgc tgcggcaggg ttatcaacca ttgcatccca
     2341 gtcgcgaaaa agcctttcct cttcctcaac gcgcgaatta aacatgctgg gatagagaca
     2401 ggcaaaaacc atctcgcgca agcgattcag atctttaact ggtttcagca aaacatcttc
     2461 aacgcccaga cgtaacgctt tggcaatatc tgccatattt tcagtggcag atatcaccag
     2521 aactggggtc tggtcgcctc tgttacgtat atgctccagc agtttaagcc cgttcattcg
     2581 tggcatcgcg atatcacata tcatcaggtc tggagtgaaa cctcccagca actcaagggc
     2641 atccacccca tcagccgcca gtaccgttgt cgctcccaat gaggaaaacc acgaatccag
     2701 aagcgagcga aatacctgct catcttcaac aatgagaatc tgttttccga ccaatggctg
     2761 cgtcatgttc tctcccctga ctggctttac tcaatagtgg catgctattg ccacctgcgc
     2821 ctgtcagaat taacttaagt gtagataaaa attctggtca aatgttggtg cgtaccaacg
     2881 gcaaaagttc gtccattttc ttttccactg ccagctgccc ggccgcaatg gcagcgtgcg
     2941 cgcgatggaa atcaagcgta gatatttgcg ggcaaacagg ttgaatcaga atatcgggcg
     3001 gatcacctgc catgcggttc cttttaaggc ggttctccag cacctggata gaagtggtca
     3061 taatctctgt tgccgttggt gctgtcaccg cccgacgtgt cgttatactg cctaaccttt
     3121 ctttcagacg cgcatgccac ggcagagaat caccattctc gctattttct tcactgacat
     3181 taaaggagag caaatcttgt tgcatcaaat gagcatcgtg ctgcaggtcg accgctatca
     3241 caatatcagc acccaatgca cgcgtgagcg aaataggaat tgggttaacg actgccccat
     3301 caaccagcca gtagccgtta tgcgcaacgg gtgccattag tcctggaata ctgcatgatg
     3361 cgcgaatagc aagatggaga tcgccttcag taaaccataa ttcacgccca gtacttaaat
     3421 tggtggcaac agccgcgaag cgacgggaac aattttcgat ctctgtttcc ggcattattt
     3481 cgcggtattg attgaagaca cgctcgccgc gcagtaaccc accgcgctgc caggagagat
     3541 ccatcagtcg taaaacatcc caataactga aagaggtcac ccaatcttcc agcgcagata
     3601 atcgatcgca tgcataggcc gcgcccacca gcgaaccaat tgaacatcct gcaacgatat
     3661 caatttcaat acccactttt tttagcgcat taataacgcc aatatgcgac caacctctcg
     3721 ccgcgccaga tcccagcgcc agccctattt ttatctttct cattattccc gcaagattcc
     3781 cctggaatgc aatcgtagcc acatcgctac tgctcggtta acatagtgct actcaggcgg
     3841 ttaatacgcc gttattgttt ccagggagat tatttgtgtc tcagctttgt ccctgtggta
     3901 gtgctgtcga gtatagccta tgttgccacc cttatgtgtc tggtgaaaag gttgcacctg
     3961 atccagaaca tctcatgcgt tcgcgttact gcgcttttgt gatgcaagac gcagattatt
     4021 taataaagac ctggcatcca tcttgtggag cagcggcgtt acgtgccgaa ttgattgccg
     4081 gatttgcgca tactgaatgg ttgggattga ccgtatttga acattgttgg caggacgccg
     4141 acaatatcgg atttgtcagt tttgttgctc gatttaccga aggtggcaaa acaggtgcaa
     4201 taattgaacg ttcgcgattt ttgaaagaaa acggtcaatg gtactatatt gacggtacac
     4261 gtccgcagtt tggccgcaac gatccctgcc cttgtggttc aggtaaaaaa tttaaaaagt
     4321 gctgcggcca ataatggttg acggtacggt ttagcaaaca ctctcaacaa ggtttttcca
     4381 gcaatgcatt cactccaacg taaagttctg cgtactattt gtccggacca aaaaggtctg
     4441 atcgcacgta ttaccaatat ttgctacaag cacgagttaa atatcgtaca gaacaatgaa
     4501 tttgttgatc accgtaccgg gcgctttttt atgcgcacgg aactggaagg gatttttaat
     4561 gattccaccc tgctggcgga tctcgatagc gcattgccag aaggctccgt gcgtgagctg
     4621 aatcctgccg gtcgtcgccg gatagtgatt ctggtcacta aagaagcgca ttgccttggc
     4681 gatttgttga tgaaagccaa ttatggcggc ctggatgtcg aaatcgcggc agtgattggt
     4741 aaccacgata ctttacgttc tctggttgag cgttttgata taccgtttga gctggtaagc
     4801 catgaagggt taagccgcaa cgagcacgat caaaagatgg cggatgccat tgatgcttat
     4861 caacctgact acgtggtgct ggcgaagtat atgcgggtat taacaccgga atttgtgtca
     4921 cgcttcccga ataagatcat caatattcac cattccttcc tgccagcgtt tatcggcgca
     4981 cgtccttatc accaggccta tgaacgtggc gtgaagatta ttggcgcaac cgctcactat
     5041 gtgaatgaca atctggacga aggcccaatc atcatgcagg acgttattca tgtcgatcat
     5101 acctacacag ctgaagatat gatgcgcgca ggtcgtgacg tcgagaaaaa cgtcttaagt
     5161 cgcgcgctct acaaagtact ggcgcagcgc gtctttgttt acggtaatcg gacgattatt
     5221 ctttaatcgc cagcaaaaat aactggttac ctttaatccg ttacggatga aaattacgca
     5281 accagttcat ttttctcaac gtaacacttt acagcggcgc gtcatttgat atgatgcgcc
     5341 ccgcttcccg ataagggagc aggccagtaa aaagcattac cccgtggtgg ggttcccgag
     5401 cggccaaagg gagcagactg taaatctgcc gtcatcgact tcgaaggttc gaatccttcc
     5461 cccaccacca tgattcacca cagcgatgtg gtaacttcgg tgagattgtg aaagtttgca
     5521 ctcatcgggg aagggtgaga accttcgact aaggttcgac tcgagcgaaa gcgagagaac
     5581 gttttcacag gcaacggccc gaagggtgag gagcgaagcg acgaataatc ctttccccac
     5641 cgccatctca agcattacct caaaatttga attacccctg gtggggttcc cgagcggcca
     5701 aagggagcag actgtaaatc tgccgtcatc gacttcgaag gttcgaatcc ttcccccacc
     5761 accatcactt tcaaaagtcc ctgaactctc aagcgaatcc gtaaataaat attctgccca
     5821 agcggagaag gatgagaagc ttcgactaag gttcgactcg agcgccagcg agagagcgtt
     5881 gccgcaggca acgacctgaa gggcgaagcg cgcagcgctg agtaatcctt cccccaccac
     5941 catcactttc aaaagtccct gaactctcaa acgaatccgt aattaaatat tctgcccaag
     6001 cggggaagga tgagaagctt cgaccaaggt tcgacttgag cgccagcgag agagcgttgc
     6061 cgcaggcaac gacccgaagg gcgaagcgcg cagcgctgag taatccttcc cccaccacca
     6121 tcgcttcctg aaataatcaa aacctgttac tccgagctaa ttttgcccaa tggcaccacg
     6181 tttaccggac ctacaatatc ctcccttgta ggtcggataa gcgaagcgca tccgacaact
     6241 caccccgcac cgtgcccata aaaaaacccc gccgaagcag ggtctatgtg aactaaaatt
     6301 cgcttagtga cgagcgcgca ccagctggta cttacgtgtc agatactcca ccggtacgct
     6361 ccagatgtgt accagacgcg agaacgggaa cagcaggaat aacgtcatcc ccagcaccag
     6421 gtgtagacgg aagataaacg ccacaccatc gaggtgttga gaagcgccac cgtggaaggt
     6481 cactaccgac tgcgcccagc caaccagttt catcatctcg ctaccgtcca tatgttgggc
     6541 ggagaacgga atggtcaaca ggcccagcgc gcactggata acgagcagcg acaggatcag
     6601 tatatccgct ccggtagtcg ttgcacgcac gcgtgggctg aacagacgac gtttaagcaa
     6661 aagcacgccg ccaatcagac acagcacgcc gctggcacca ccagcaaaca tcgccatttt
     6721 ctgtttcact tcaatcggca gccaggcttc atacatccag tgcggcgtca acataccgaa
     6781 gaagtgaccg acaaaaatcc ccagaatacc gatatggaac aggttcgacg ccaggttcat
     6841 ccctttgcga tccagcatct ggctggacgc cgcgcgccag gtgtactgcc cgtagtcata
     6901 acgcagccag ctaccaatca ggaagaccgc cccggctatg tacgggtaga tatcaaagaa
     6961 gaacatattc aggaattgca ttagtgctgt cctccggtgg tgatattcag atattgcggc
     7021 gcgacggcac cggcaaagcg acgctgatga gcagtgattg ctgaatcacc gcagcctttg
     7081 tcagcaaaga atttaacctg ctcttcttcc caaacagcat ccagcgcctg cggcgtatca
     7141 tcgcgcgctt cgtcggcaat tttttccgcc actttgtcgc tgtcgatagc ggtattggcc
     7201 aatttcagca gcagatcaaa cagcacggca taacggcttt cacgctgttg cagacgcgca
     7261 ctcagcaatg ccagaatcgg cgcgatatct ttcaaacctt ccacggcttc gctttgcggc
     7321 agctgcgcca ggtactccag atacagcggc agatgatccg gcaattcgcg gctgtttaac
     7381 tgcaagccgt gctgctcgta ctgcgccagc aggtccacca tcgcctggcc gcggtcgcgg
     7441 gattcgccgt gcacatgttc aaacagcaac agtgacgtgg cgcggccacg gtcgaacagt
     7501 tcgctgtact gcgcctgggc atcgagcgga tccatcgtcg ttaaatcgcg caggaaaatg
     7561 cccagcgcat gggcatcctc ttttggcaga tttttcgacg cggcaatcgc ctcaaacatc
     7621 tcttgttgat gctgccataa ggcagcatcc ggatattcaa ggagacgcga tacaatcacg
     7681 agttcgatca tggatgcggc tccgttttgc tggtcacatc gatggcatcg atacgacggc
     7741 tgttgaacag attgaatttg gtatctgaac cgtggcagcc atcaccaaag gtaaagccgc
     7801 agccattttt ctccgggaag gcttcccgtg ccagttcacg atgactactc ggcaccacaa
     7861 agcgatcttc gtagttagca atcgccagat aacggtacat ctcctgtgcc tgggcttcgg
     7921 tcagaccgac ctcttccagc gcacgggtat caactttacc gtcaacggtt tcagcacgtt
     7981 tgtaatgacg catcgccagc atacgtttca gcgcgcgcag taccggtttg gtatcaccgg
     8041 cggtcagcag attcgccaga tactgtaccg gaatacgcag gctttcgacg tctggcagaa
     8101 tgccgttgct acccagctca cccgcgtctg ctgcagactg aatcggagag agaggcggca
     8161 cgtaccagac catcggcagt gtgcgatatt ctggatgcag cggcagcgcc agtttccatt
     8221 ccattgccat tttataaacc ggcgactgct gtgcagcttc aataacgctc agcggaatac
     8281 cgtctttaat cgcctgctcg atgactttcg gatcgttcgg atcgaggaac acctccagct
     8341 gacgctggta gagatctttc tcgttctcgg tgctggctgc acgttcaatc gcgtcggcat
     8401 cgtacagcag cacgccaaga taacggatac gaccgacaca ggtttctgag cacacggtcg
     8461 gctgacccgc ttcaatacgc ggatagcaga agatgcactt ctcagactta ccgctcttcc
     8521 agttgaagta gatttttttg tacgggcatc cggtgatgca catacgccag ccacggcatt
     8581 tatcctggtc gatcaggacg atgccatctt cttcacgctt gtaaatcgca ccgctcgggc
     8641 aggtcgccac acatgccggg ttcaggcagt gttcgcacag gcgcggcaaa tacatcatga
     8701 aggtgttttc gaactggcta tacatcgcct tctggatgtt gtcgaagttc ttgtctttcg
     8761 ccagtttgtc aaactcaccg cccaggtcat cttcccagtt cggccctttt tcgattttcg
     8821 ccatccgttc cccggtaatc agcgaacgcg gacgggcaat cggctgcgat ttgctgcctt
     8881 ccggcgcggt atgcaggttc tgatagtcaa aatcgaacgg ctcgtaataa tcgtcgatcc
     8941 ccggcagatg cgggttagcg aagattttac ccagcagcat ggcacggtta cccatgcgcg
     9001 gttgcagttt gccgttgatt ttacggatcc agccgccttt gtatttttcc tggttttccc
     9061 agtcagtcgg gaagccctgg cccggcttgg tttccacgtt gttgaaccac gcgtattcca
     9121 cgccttcacg gctggtccag acgtttttac aggtaactga acaggtgtgg cagccgatgc
     9181 acttatcgag attcagcacc atgccgactt gtgaacgaat tttcatttta cgctctcctg
     9241 tacctgatca ttgccttcgc catctaacca gtcaatgttc ttcatcttac gcactacaac
     9301 aaactcatcg cggttagaac ctacggtgcc gtagtagtta aagccgtatg ccagatgggc
     9361 atagccgccg atcatatgcg tcggtttcgg cgtgatacgg gtgaccgagt tatggatacc
     9421 accacgctgt tgggtaattt ccgaaccagg caggttaacg atacgttcct gcgcgtggta
     9481 catcatggtc atccctgccg gaacacgctg gctgacaacc gcacgggcag tcagagcacc
     9541 gttgctgttg aatacttcaa tccagtcgtt atcggcgata cccagatctt tggcatcggc
     9601 ttcactcaac cagaccaccg gaccaccgcg acctaaagtc agcatcagca ggttgtcgct
     9661 gtaggtggag tggatacccc acttctggtg cggcgtcagg aagttgagcg ctttttccgg
     9721 gttgccgttg gatttctggc ctatcacttc tttcaccgaa cgggtgtcga tcggcggacg
     9781 ataaaccagc aggctttcac cgaaatcacg catccactgg tgatcctgat acagttgctg
     9841 acgaccagag agcgtacgcc atgggatcag ctcgtgaacg ttggtgtaac cggcgttgta
     9901 agaaacgtgt tcatcttcca gaccagacca ggtcgggctg gagataattt tgcgcggctg
     9961 tgcctgaata tcgcggaagc ggatcttctc gtcttcttta ttcagcgcca gatgcgtatg
    10021 gtcacgaccg gtaaattcgc tcagggcagc ccaggctttc acggctacct gaccgttggt
    10081 ttccggtgcc agtgtcagga tcatctctgc cgcatcaatt gcggtgttca gcatcggctg
    10141 gcctttcgcc ggaccttccg ctttggtgta gttgagctta cgcagcagat ccatctcgct
    10201 ctgggtgttc caggcaatcc ctttaccgcc gttaccgatt ttctccatca gcgggccgat
    10261 agaggtaaag cgttcgtaag ttgccggata atcgcgctct acgaccataa tgtgcggtgc
    10321 agttttgcct gggatcaggt cgcattcgcc ttttttccag tctttcacat ccagcggctg
    10381 cgccagttca gcggcagagt cgtgctggat aggcagcgtg acgatgtcgg tttctttacc
    10441 cagatggccg acgcacactt cggagaattt cttcgcgatg gctttgtaga tttcccagtc
    10501 gcttttcgct tcccaggccg gatcgaccgc cgcagacagc gggtgaataa acggatgcat
    10561 atccgaagta ttcatgtcgt ctttttcgta ccaggtcgcc gtcggcaaaa tgatgtcgga
    10621 atagagacag gtgctcgaca gacggaagtc cagcgtaacc accagatcca gcttgccttc
    10681 cagaccattg tcctgccagt ccacttcttc cggcttcacg ccgccctgct gccccagatc
    10741 tttaccctgg ataccgtgct ccgtccccag caggtacttg agcataaact catgaccttt
    10801 accggaagaa ccgagcaggt tagaacgcca gataaacagg ttacgcgggt ggtttttgcc
    10861 gttttccggc tgttccgcag cgaaacggat agaaccctct ttcagggatt tcaccgtata
    10921 gtccaccgga ttcatcccgg ctttttctgc ttcgccagcg atagtcagcg ggttagtgcc
    10981 taactgcggc gcagacggca gccagcccat gcgttctgca cggacgttaa agtcgatcag
    11041 atgtccggta tagcgggatt tatccgccat cggcgacagc agttcttccg cagtcacggt
    11101 ttcataacgc cactggctgg agtggttata gaaataagag gtgctgttca tgtgacgcgc
    11161 cggacgctgc cagtcgaggg caaacgccag cggctgccag ccggtttgcg gacgcagttt
    11221 ttcctgacct acatagtgcg cccagccgcc cccgctctga ccgacacagc cgcagaaaat
    11281 cagcatgttg atcagaccac gatagttcat atcgaggtga taccagtggt tcagccccgc
    11341 accgacgata atcatcgaac gaccgtgcgt tttatcagcg ttatcggcaa attcacgggc
    11401 gatgcgaata atctggctgc gagaaacgcc ggtaatctgc tcggcccagg ccggggtata
    11461 agctttcaca tcgtcatagc tggttgcaca gttaacgtcg ttcaggccac gttccagacc
    11521 gtagtttgcc agcgtcagat cataaacggt ggtcaccagg gcggtgctgc catcagccag
    11581 ttgcaggcgt ttcaccggca gtttgtgcag cagcacgttt tccagttcca ctttgttgaa
    11641 gtgttccgtg ccgtcgccac caaagtacgg gaagcctacc tcagcgatct catcctggct
    11701 acccagcagg ctcagttgca gctcggtttc ttcgccagtt ttgccgtcgc gctgctcaag
    11761 attccatttg cccttctcgc cccagcggaa gccaatagaa ccgttcggcg caaccatttc
    11821 gccattggta ttaaaggcga cagttttcca ttccggattg ttttcctggc ccagcgcatc
    11881 aaccagatca gcagcgcgca gcatacgacc tgcagcgtag taaccgtcgc gttcttccag
    11941 catcaccagc atcggcatgt cggtgtagcg acgcacatag tcggtgaaat actggcttgg
    12001 gttgtcgaga tggaattcac gcagcattac gtggcccatc gccagcgcca ttgccgcatc
    12061 ggtgccctgt ttcggtgcca gccacagatc gcacagtttg gcgatttcag cgtagtctgg
    12121 tgtgacggca acagttttgg tgcctttgta acgcacttca gtaaagaagt gagcatctgg
    12181 ggtacgcgtc tgcggcacgt ttgaacccca ggcgatgatg tagctggagt tgtaccagtc
    12241 agcagattcc ggtacgtcag tttgctcgcc ccaggtttgc ggagacgcag gaggcaggtc
    12301 gcagtaccag tcgtagaagc ttaagcaagt accgccaatc agcgagagat agcgtgcacc
    12361 cgatgcgtaa gaaaccatcg acattgccgg aattggcgag aaaccagcaa cacggtccgg
    12421 gccgtagttt ttgatggtgt aaacgttaga tgcggcgatc agttcgttca cctcctgcca
    12481 ggaagaacga acaaaaccac cgcgtccacg cgcctgctta aagcttttcg ctttatcggc
    12541 gtcttcaatg atagaagccc atgcctcaac cggatcgcta tgcagcgcct tcgcttcacg
    12601 ccacattttc atcaggcgtt tgcgcatcat cgggtatttc aggcggttgg cactgtaaag
    12661 ataccaggag tagctggcac cgcgcgggca gccgcgaggt tcatggtttg gcagatccgg
    12721 acgggtacgc ggatagtcag tctgctgggt ttcccaggtg accagaccgt ttttgacgta
    12781 gattttccag ctgcaggagc cggtgcagtt taccccgtgg gtagagcgga cgattttgtc
    12841 atgctgccaa cgctggcgat atccatcctc ccagtcacgg ttggtattga gaagctggcc
    12901 atgcccatcg gcaaaggttt cacccttctg tttgaagtag cgaaaccggt ccaggaattt
    12961 actcatcggt tttctcctgt gggagcctgt cggccctctg ataaatcgac attgctaatg
    13021 tttaagggca aggtaacgct ctgaaacagc gtgggaattg ataacgatca agagtgatgg
    13081 ggatgaaaaa taaagtaaat tcctaagata cccctttagg ataattaaca gttacagcat
    13141 aacatgctgt aaattaagac gttttatacc tgtagttatt taaactatta agatttttat
    13201 gcaaatgg
//
LOCUS       DAZKIF010000123        13204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZKIF010000123 DAZKIF010000000
VERSION     DAZKIF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.124"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..442
                     /locus_tag="ACHOTW_003742"
     CDS             <1..442
                     /locus_tag="ACHOTW_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFP9845769.1"
                     /translation="SNILFDYAWFDNTIFIKTLFKNTCFYNVRAKNVYLEGAYLNNDN
                     IVNQANNSTEKQSIDSTDKQANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVNDSTD
                     KQAKNSTEQQDSNSFNQARLKKEVNRRFSIPGLTSYQPTYIVEE"
     gene            708..1550
                     /locus_tag="ACHOTW_003743"
     CDS             708..1550
                     /locus_tag="ACHOTW_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP9845770.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            1618..2796
                     /gene="yjiJ"
                     /locus_tag="ACHOTW_003744"
     CDS             1618..2796
                     /gene="yjiJ"
                     /locus_tag="ACHOTW_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9845771.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            3007..8248
                     /gene="iatD"
                     /locus_tag="ACHOTW_003745"
                     /pseudo
     CDS             3007..8248
                     /gene="iatD"
                     /locus_tag="ACHOTW_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(8405..9000)
                     /gene="kptA"
                     /locus_tag="ACHOTW_003746"
                     /pseudo
     CDS             complement(8405..9000)
                     /gene="kptA"
                     /locus_tag="ACHOTW_003746"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            9205..9888
                     /gene="yjiH"
                     /locus_tag="ACHOTW_003747"
     CDS             9205..9888
                     /gene="yjiH"
                     /locus_tag="ACHOTW_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP9845772.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9885..10346
                     /gene="yjiG"
                     /locus_tag="ACHOTW_003748"
     CDS             9885..10346
                     /gene="yjiG"
                     /locus_tag="ACHOTW_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP9845773.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10359..11531
                     /gene="iadA"
                     /locus_tag="ACHOTW_003749"
     CDS             10359..11531
                     /gene="iadA"
                     /locus_tag="ACHOTW_003749"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9845774.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11653..12507
                     /gene="hypT"
                     /locus_tag="ACHOTW_003750"
                     /gene_synonym="qseD"
     CDS             11653..12507
                     /gene="hypT"
                     /locus_tag="ACHOTW_003750"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP9845775.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(12500..12892)
                     /gene="iraD"
                     /locus_tag="ACHOTW_003751"
     CDS             complement(12500..12892)
                     /gene="iraD"
                     /locus_tag="ACHOTW_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP9845776.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12889..12972)
                     /gene="idlP"
                     /locus_tag="ACHOTW_003752"
     CDS             complement(12889..12972)
                     /gene="idlP"
                     /locus_tag="ACHOTW_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFP9845777.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(13088..>13204)
                     /gene="ytiD"
                     /locus_tag="ACHOTW_003753"
     CDS             complement(13088..>13204)
                     /gene="ytiD"
                     /locus_tag="ACHOTW_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFP9845778.1"
                     /translation="FPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         3458 a         3294 c         3194 g         3258 t
ORIGIN      
        1 ttcgaatatc ctatttgact acgcatggtt tgacaataca atatttataa aaacgctttt
       61 taaaaatacc tgtttttaca atgtcagagc gaaaaatgtc tatcttgagg gagcatatct
      121 gaacaatgat aatatcgtga atcaagccaa taacagtacc gagaaacaat ccattgacag
      181 taccgataaa caggccaatg acagtacggt gcaacaatcc attgacagta cggtgcaaca
      241 agccaatgac agtaccgata aacaagccaa tgacaatatc gataaacagg tcaatgacag
      301 taccgataaa caagccaaga acagtaccga gcaacaggac agtaacagtt ttaatcaagc
      361 ccgtttaaag aaagaagtga ataggagatt ttccattccg ggtttaacgt cttatcagcc
      421 aacatatata gttgaagaat agttcattaa tcacccaaac agagtgggta tggcagacga
      481 tatagattaa catttaaacg ttaaccgcct catctatcat tcatagatga ggcgcattta
      541 tcagtcgatg taagctgaaa atgttctgcc atccagagac aaccgccgcc ccctgcggcg
      601 tttactattt catcgcccgc gttaagaatt cattaagctt catccgtata ctccttatct
      661 gtcgcactga atttgacgag aaaaaagcaa taataaggag caataagttg aaaaagcgca
      721 tcacctttat cgtttttctt tgcgccatcg tggcggcaag cctgtttttt gtgcaatcct
      781 gcgtccgtaa aagccagcat gtcgcagggt ttcagaatta tcaggccact atcgatggta
      841 aagagattac aggagtaact aaaaatatct cctcactgac ctggtcagcg cagagcaata
      901 ccttatttag cactatcaac aaacctgcaa ccatcgttga aatgacgacc gagggcgact
      961 tgatccgcac cattccgctc gattttgtta aggatctcga gacgattgaa tacattggtg
     1021 ataataaatt tgtcatcagt gatgaacgtg actacgccat ttatgtgatt tcacttaacg
     1081 ctgactcgga agtgagcatc ctcaaaaaaa ttaaaatccc attgcaggaa acaccaacta
     1141 actgtggctt tgaagggctg gcttactcct ctcaggacca taccttctgg ttttttaaag
     1201 agaaaaatcc gatagaggtc tacaaagtaa ccgggctatt gcgtagcgat gagttgcata
     1261 tcagcaaaga taaaacgttg cagcgtcagt ttactttaga cgatgtttct ggcgcggaat
     1321 ttaacccaca gaaaaatacg ttgctggtgt tgtcacatga gtcacgcgcc ctgcaggaag
     1381 tgaccgtgag aggtgacgtg atcggcgaaa tgtcgttaac caaaggtaag tatggccttt
     1441 cacacaacat caagcaggcg gaagggatag cgatggatga tagcggcaac atctatatcg
     1501 ttggcgaacc gaatcttttt tatcgcttta cgtcgacaaa atcacgataa cagtctggca
     1561 ggttctcctt ttttgttatt aacttgttaa ctcaaaataa tacaaaccag gcagaacatg
     1621 aattcgacat cgcatcccgt agaacgtttt tctttcagca ccgcgttatt cgggatgctg
     1681 gttctgacct taggtatggg tttaggccgc tttctctata cgccgatgct gccagtcatg
     1741 ctggcggaag gcgagttttc atttagcgaa ctctcatgga tcgccagtgg taactatgcc
     1801 gggtatctgg cggggagcct gctgttttca ttcggcgcgt ttcatttacc ctcacgcctg
     1861 cgcccgttcc tgttagcttc cgccctcgca accggattat taatcctcgc gatggcgtgg
     1921 ctgccgccgt ttcttctggt tttcatcatt cgctttctgg cgggagtcgc cagcgccggg
     1981 atgttgattt tcggctcaac actcatcatg caacatactc gccatccctt tgtccttgcg
     2041 gcgctatttt ctggtgttgg cgtcggcatc gctctgggta atgaatatgt gctggcaggc
     2101 ctgcattttg ccctctcttc acaaacgttg tggcaaggtg ccggagcact ttctgccatt
     2161 atattgcttg ctctggcgct gctcatcccg tcgaataaac acgttatccc gccagcgcca
     2221 ttggcaaaaa tcgcgcaaca acccatgagc tggtggttac tggcgattct gtatggtctg
     2281 gcgggttttg gttatatcat cgtcgccacc tacctgccgc tcatggcgaa agacgcgggc
     2341 cagcctgtgt tgacggctca cctctggaca ctggttggct tgtcgattgt cccaggttgc
     2401 tttggctggc tgtgggcagc caaacggtgg ggagcattac cttgcctgac cgcgaatttg
     2461 ctggtgcagg cgatctgcgt gctgttaacc ctcgccagca gctctccttt attactcatc
     2521 atcagcagta ttggttttgg cggcaccttt atgggaacga cctcgctggt gatgaccatc
     2581 gcccgccagc ttagcgtgcc gggaaatctt aaccttttgg gctttgtgac actcatttat
     2641 ggtatcgggc aaattcttgg cccggcgctg accagtatgc tcggcaacgg aacgtcggcg
     2701 ctcgccagcg ccacactctg cggcgcagcg gcgctattta tcgcagcatt aatctgcggg
     2761 atgcaaatat tcaaattgca tacgaattat tcttaagtaa tttaactaat ttaacttttc
     2821 agcacatcac gtaaaataat taaaatatcc tttttctttg gaggtactca gccaccgccg
     2881 gtattattcc tggtttagca attccacggc taattcatta tttctggtca tattatagtc
     2941 aggatcgcat tatatctccg tgctagaaaa aacaagttta attctgtttt ttcacggaag
     3001 gtcactatgg cttcgacaaa tgcgcatcaa ataaaaaata atgatcaaaa ctctttatgt
     3061 ggtctgggtg acaaaatacg ccgcctgacc gccggagttt gtttatttac acaatttttt
     3121 ttccccgtca tggcgacggc acaaaatgtg gtacatgcta aacctcagac gacagtttca
     3181 tctgcccccc cctctactag aaaataatac tgtgccttac acgcttggtg cgctggaatc
     3241 agcgcaaagc gttgctaatc gcttcggtat ttcactggag gagcttcgtc gtcttaatca
     3301 gttccgtact tttgctcgcg gctttgataa cgtgcgccag ggtgaagaac tggatgttcc
     3361 ggcaacaacc tcgcagaaaa gccatgagca acaaaatgcc gtaccgcctg cgaatggcga
     3421 aaacacgctg gagaatcaaa tagccagcac ctcgcagcga gttggcactc tgctttcaca
     3481 agatatgaat agtgagcagg ccagcggcat ggcgcgtggt tgggcgtctt cagaagcctc
     3541 aggcgcgatg actgattggt taaataactt tggtactgcg agaatctctc tgggtgtgga
     3601 tgaagatttt agcctgaaaa attcgcaatt cgacttcctg catccgtggt atgacacacc
     3661 tgattatctg ctcttcagcc agcataccct tcaccgaaca gacgatcgta cccagatcaa
     3721 caccggtttg ggctggcgtc atttcacctc cagctggatg tcaggcatca accttttttt
     3781 tgaccacgac ctgagccgct atcactcccg cgcagggctt ggcgcagaat actggcgtga
     3841 ttatctgaag ttgagcagca acgcttatat cggcctgacc ggctggcgta gcgcaccaga
     3901 attggataac gacttcgaag cccgcccggc caacggctgg gatttacgcg cggaaggctg
     3961 gttacctgcc tggccacaac tggggggaaa actggtctat gaacaatact atggcgatga
     4021 agtggcgctg tttgacaaga atgatcgtca aagtaacccc catgctatta ccgcaggcct
     4081 caactatacc cccttcccgc ttctgactct cagtgcggaa cagcgtcagg ggaagcaagg
     4141 tgaaaatgac acacgttttg ccgttgatct gacctggcaa cccagcagtt caatgcagaa
     4201 acagcttaat ccggacgaag tggccggacg gcgcagtctg gccggtagtc gttatgacct
     4261 gattgatcgc aacaacaaca tcgttctgga ataccgcaag aaagagctga ttcgcctgag
     4321 tctgctggat ccggtgaaag ggaagtctgg agaaataaaa ccgctggttt cctcgctaca
     4381 gaccaaatat gcccttaaag gctataacat cgaagccgct gcgctggaag ctgccggagg
     4441 taaagtcagc acgtctggaa aagatatcac ggtcacgctg ccaggttacc gcttcactaa
     4501 caccccagaa accgataata catggtcgat agacgttacc gccgaggatg taaaaggtaa
     4561 cctgtcacgg catgaacaaa gcatggtagt tattcaggct ccgacattaa gccagaaaga
     4621 ttctctgtta tccgtcaatc cgctaaccgt ggctgcagat aaaaaatcga cgaccacatt
     4681 gaccgttact gcgcacgatt ccgacggaac tccggtgccg gggctggcgc tgcaaacccg
     4741 cagtgaaggc gttcaggata tcaccctgtc tgactggaca gataacggtg atggtagtta
     4801 cacacagata ctgaccgccg gaacgacatc aggttcagta acactgacgc cgcaaattaa
     4861 cggtgagagt gcggtaaaag aatccatcgt cgttaatatc gtccctgttg tctcatcccg
     4921 cgaccattca tcaataacaa ttgataacgt atcgtattat gccggagacg acatcaaggt
     4981 tagggtggaa ctgaaagacg atagcaatca accggttgca tatcaaaaag aggaattggt
     5041 aaaagccgtt actgtcgaaa acagcaaacc tggcgccacg attgtctggc acgaagagca
     5101 gccgggggtt tatgccgcga attatccggc ctataagcaa gggactgcac taagggcaca
     5161 acttagcctt cacaactgga atgctccact gcaatcgcat atttataaca ttgaggcaaa
     5221 ccagaataag gctcgcgttg ccacattatc agcgacaaat aatgacgttt acgccgataa
     5281 aaagacattt aataccctca cgatcaacgt cactgatgag agtgataatc ccctgacaaa
     5341 tcatcaggtc acctttaaga atgaaaaagg aagcgcggag tttgtcgaac cgccgcagca
     5401 aaatacggat gcatatggtg ttgccacaat aaacatggta agtcaggttg cggaagaaaa
     5461 tacgattagc gccacgctgc caaatggttt ttcacaacgg ataattgcga aattcgttag
     5521 cgattcgagt acgccaaaat tcaaacaact ggttgccgat ccagatacca ttattgctgg
     5581 caacagccag ggcagtactc tgaccgccat catcacagac tttcataaca acccgttaaa
     5641 agatatgaaa gtgaattttg tggcacctgg tggctcgcaa ctggacaaca cgaccgccac
     5701 aacagaccag tccggtattg tgcgggtgca cctgaccagt tcaaaagctg gtagctattc
     5761 cgtcgatgcc tcgcttgagg tggataaaaa tattcaccag tcggtcacga tcaccgtggt
     5821 cccaaacagg gaacaatcgg taatgacctt gaatgccggg tcgggcagtg cgatcgctaa
     5881 caatacaaat atcgttaccc tgactgccag tgtgaaagat gtttatggac acccgttgcc
     5941 ggatgaggat gtgaaattta ccttgccagc ctccatgacc gggaacttca cgctaagtag
     6001 tgaaaccgcc cgcaccgatg caaacggtga tgccgtggtc acattgcgag gcacaaaagc
     6061 gggtgagttt acagttacgg cgacgctgac cagaaataat accgttgctt atcagcaagt
     6121 cacttttatt ggggatacaa acagtgcgca gctccagccg ctgactgcct cattaaattc
     6181 cattgttgcg ggtaacagta cggggagtac cctgacggca acgatcctgg acgcttacca
     6241 aaatccgctt aaagaccagt tggtcacttt ccagagtaac gatgtcactc taagcgaaac
     6301 agaagtcacc accaatacgc tgggtcaggc gacggtaaca atgaccagca atattgccgg
     6361 acaacataac gtcgtggtga gccggaaagc gcaagcttcc gataataaaa cgtttagttt
     6421 atcagtgcta ccggatgaaa gttcggcgaa ggtaataagt ataaccggag ccgaaaaaac
     6481 gataacggtg ggcgaaaaca tcacgctacg gatactcgtc caggacgcgt ttaacaatgt
     6541 aatcgcgggt caacgcgtca gattaagtgc gcagccaaca actaacatta cgataggcga
     6601 tacggcttac accgataata acggttatgc gtacgttaac cttctcagca cccaacctgg
     6661 ggtttatcag gtgacggcaa cgctggacaa taacagtagt agtaaggttg acgtgaatgt
     6721 ggcaaatggc aaactcgagt taacatcatc gaaaccagaa actacggtcc ataatagtga
     6781 gggtattacg ctgaccgcaa cggcgagaaa tgcgcggggt gaattgatgc cagggcaaat
     6841 tatcaccttt agcgtaacgc ctgaaggtgc aacgctaagc aatacagggg aagtccttac
     6901 tgaccagtca ggtcaggcca aagtgacgct gaccagtgac aaagtgaatg tctataccgt
     6961 tacggccata atgggcaaag atgttcccgt tcagagccag gtaacggttg cggttaaggc
     7021 agatgctaaa acggcacatg ttgtgagcgt cgtggcttct cctgacacca tcaccgccga
     7081 cggcatcgat agcagcacca tcacttcacg agtagaagat gattacggat tcccggttga
     7141 aggtgtcgat attagtcatg gcttagacac caaaggcagc ccggtagtta atattccaac
     7201 tacgcgtacc gatcagtccg ggcaagtcac ggcgacaata accagtacat tggcagaaac
     7261 cttaacagtc aatgtgcaag ttcctggcac agccaaccaa tccgcaacca ttacattggt
     7321 tgccggcacg gccgatgaaa gtaagtcaat tttgaaatcc gatgttgaca ctctgaaggc
     7381 tgactaccag cagagcgcaa aacttacgct aacattgcaa gacaagtacg gtaacccgat
     7441 agtgacgtct gatcatctgg aatttgtcca gtcaggcccc ttcgtgaact ttctcaagtt
     7501 gagcgatatt gattacagcc aaagaaatta tggcgagtac accgtgactg tcactggcgg
     7561 aaaagaggga acagcgacac tcattcccat gctgaacggg gttcatcagg caaacttaag
     7621 catatcgctg aatctcatcc aatcgataaa agaaatgtcc ggtcatgtca ctgcaaacaa
     7681 ccataccttc tccacggcta aattcccgag cgaaggcttt gcaggagcgt attacacact
     7741 caacaatgat aactttgaag cgggtaaaac cgttgatgat tatatgtttt caagttcaca
     7801 gggttgggtg tctgtcgatg cttcgggtaa agtttctttc gcaaatatcg gcgatcaaac
     7861 gtcagtcaca ataagcgctg ttccccgaca aggaggtaca acctaccaga ccttaattaa
     7921 gctgaaaggc tggtgggtga ataatggaaa tcataccaat atctggctag ctgccaatgc
     7981 gctctgtcat gctaaaaatg atggatataa tcttcctggc atcacacatt tgacgtctgg
     8041 cgaaaacaaa cgcacgcagg gatcactgta tggtgaatgg gggaacgttg gagcgttttc
     8101 cagtaattcg caatttacac cgggagctta ctggacaagt gaatctgatg attacagtcg
     8161 gcactactat gtgcagatgc taaccggtat gaccggaagc gacgctgatt ccagccccca
     8221 actgaccgcc tgccgtaaat cactttaatt tcagcagttg ttattaaagg ataccctttt
     8281 acgagggtat cctttcagac tgataacaaa cgctacattg ccggatgtgc tgcgcttatc
     8341 aggcctatat aacccctgca atatattgaa tttattgttt ttgtacccca gccggcataa
     8401 acgactactc aagaaactca acagcaaccg ttgatgtcag ccagaccccg ttttccgctt
     8461 gccagaaagg gatgcctcgt ttcgccattt cctgtgcttt gacggttaag acaaccggcg
     8521 atccgtgtct tgctcccact ttgcgtgccg ttgcttcatc agcagagaga tgcacgtaat
     8581 gacgatcccc tgcaatcagc ccctgttttt ttatctcgtc aaggaaacgg cttgccgtac
     8641 cgtggtagag aaactgtggc ggtgtttttt cggcaaagga aatagcgacc tgcgaagtcg
     8701 aatgcccctg aaccgcgcgg atgcacctac catcactgga ataactaaaa cgcttttatc
     8761 gctggttgcg accacggtat ccagcaatgc acgggttaat cgcttgcccg ctttttgggc
     8821 acaaagtatg agcttatcga tattcgccca tccttcacgg tccagtacga taccaatcgc
     8881 ctccggtttg tgtcggagga caaaacttaa aaacttgctc gtatccgcca gctctttttc
     8941 gttgtacttt gccatcgcgt tttcgtccct ggattttctg tttttattta ccaatgtcat
     9001 tgtcactggg tttatgaatt ttctgcaact caatcctgcg cagaaccttt tccacacagc
     9061 gtaatttttc ccctatgcaa aaaacgcata gccagaaaca tacttgcatt ttccccatac
     9121 ccacatggct gataaggttt ttaagttcca ggaattttcc tggctcaagc aacacacaaa
     9181 cacaaaaaaa caatggggat tgttatgaca caacaagggg atgctgtagc cggggagctg
     9241 gcgacagaaa aagtgggcat taaagggtat ctggcttttt ttctcaccat aatctttttt
     9301 tccggtgttt tttccggaac agacagctgg tggcgcgttt ttgatttttc cgttctgaac
     9361 ggatcgtttg ggcaactgcc tggtgcgaat ggagccacca cctcatttcg cggcgcgggt
     9421 ggcgcggggg ctaaagatgg ctttctcttt gcgctggagc tggcaccgtc agtcattctc
     9481 tctctgggga ttatttcaat taccgatggt cttggtggat tacgcgccgc gcagcagtta
     9541 atgacacctg tgctaaaacc gttgttgggc attccaggga tctgctcgct ggcgctgatc
     9601 gctaacttgc aaaacactga tgccgccgcc ggtatgacca aagagctggc gcaggaaggt
     9661 gaaattaccg aacgcgataa agtgattttc gccgcatatc aaaccagcgg tagcgccatc
     9721 atcaccaact acttctcttc cggcgtcgcg gtgttcgcct ttctgggcac gtccgtaatc
     9781 gtacctttag ccgtcatcct ggtgtttaaa tttgtcggag ccaacatttt gcgcgtctgg
     9841 cttaacttcg aagaacgtcg caacccaacg caaggagcgc aagcatgaca actcaggtcc
     9901 gcaaaaacgt catggatatg tttatcgatg gcgcgcgccg tggctttact atcgccacga
     9961 ccaacctgct gccaaacgtg gtgatggcgt ttgtcatcat tcaggcgctg aaaattaccg
    10021 gcctgctcga ctgggtgggg catatctgtg aacccgtaat ggcgctgtgg ggattacccg
    10081 gtgaagcggc aaccgtcctt ttagccgcat taatgagtat gggcggcgca gtcggtgttg
    10141 ccgccagtct ggcaaccgct ggcgcattaa ccggtcacga tgtcaccgtt ttactgcccg
    10201 cgatgtattt aatgggcaac ccggtgcaaa atgtcggtcg ctgcctgggg acagccgaag
    10261 tgaatgccaa atattacccg catatcatta cggtctgcgt gattaacgca ttgctgtcga
    10321 tctgggttat gcagcttatt gtttaacaag gagttaccat gattgattat accgcagccg
    10381 gttttaccct gctgcaggga gcgcatttgt atgcgccgga agatcgggga atttgcgatg
    10441 tcctcgtcgc taacggcaaa attatcgccg ttgccagtaa tatcccttct gacattgtac
    10501 cggactgcac ggttgtcgat ctcagcgggc agatcctctg cccaggtttt attgatcaac
    10561 acgtccattt gattggcggt ggcggcgaag caggtcccac gacgcgcacg ccggaagtgg
    10621 cgctaagtcg cctgacggaa gcgggcgtca cgtcagtggt tggtctgctg ggcaccgact
    10681 ctatctctcg ccacccggaa tccctgctcg ccaagacccg tgcgctcaat gaagaaggca
    10741 tcagcgcctg gatgctgacc ggcgcttatc atgtcccttc ccgcaccatt acgggttccg
    10801 tggaaaaaga cgtggcgatt atcgatcgtg tgattggcgt gaaatgtgcc atctctgatc
    10861 accgttctgc cgcaccggac gtttatcacc tggctaatat ggcggcagaa tcccgcgttg
    10921 gcggtttgct cggcggtaaa cctggcgtca ccgtgttcca catgggcgac agtaaaaagg
    10981 cattacagcc tatttatgac ctgctggaaa actgcgatgt gccgatcagc aagctgctgc
    11041 cgacccacgt taaccgcaac gtaccgttgt ttgagcaggc gctggagttc gcgcgcaaag
    11101 gcggcaccat cgatatcacc agcagcattg acgaaccggt cgcccctgcc gaaggtattg
    11161 tccgcgccgt tcaggcgggt attccgctgg cacgcgtcac cctcagctcc gacggcaacg
    11221 gtagccagcc gttcttcgat gatgaaggga atttaaccca tatcggcgtt gcaggttttg
    11281 aaacgttgct ggaaaccgtg caggtgctgg ttaaagacta tgatttcagt atcagcgatg
    11341 ccctgcgccc gctcaccagt agcgtagccg gtttccttaa cctgaccggg aaaggcgaaa
    11401 ttctgccagg caatgacgca gacttactgg tcatgacgcc agaattgcgc attgagcagg
    11461 tatacgctcg cggcaaactg atggtcaaag acggcaaagc ctgcgtgaaa ggaacgtttg
    11521 aaacggctta aacggttgtc agccgcccta atggacgtag aatgccccca agggcggctg
    11581 acagagtaaa acgtaatgga tgactgtggt gcggttttgc ataatattga aaccaaatgg
    11641 ctttaagatt ttctgaccct ggaaaaatgc cgcaattttt cccaggcggc agtcagtcgt
    11701 aacgtctcgc aaccggcatt cagccgccgc atccgcgcgc tggaacaggc gattggcgtt
    11761 gaattgttta accgccaggt gacgccgctg caactctcag aacaagggaa aatcttccat
    11821 tcgcagatcc gccatctgtt gcaacagtta gaaagcaacc ttgcagagct gcgtggcggc
    11881 agcgattacg cgcaacgtaa aatcaagata gccgctgcac actctctttc cctcggtctg
    11941 ttaccgtcca ttatcagcca gatgccgccg ctctttacct gggcgattga agccattgat
    12001 gtcgatgaag cggtcgataa actgcgtgaa ggacaaagtg actgtatttt ttcctttcac
    12061 gacgaagatt tgctggaagc gccgtttgac cacattcgct tatttgaatc tcaattgttc
    12121 cctgtctgcg ccagtgacga acatggagaa gcactttttg acctcgtgca gccacacttt
    12181 ccgttactga attacagccg caactcctac atggggcgat tgattaatcg caccctgacg
    12241 cgccacagtg agttaagttt cagcaccttt tttgtctctt cgatgagcga gcttttaaag
    12301 caggttgccc tcgacggctg tgggattgcc tggctgccgg agtatgccat ccaacaagaa
    12361 attcgcagcg ggcagctcgt tgtgctgaat cgggacgaac tggtgatccc gattcaggcc
    12421 tacgcatacc ggatgaatac ccgcatgaat ccagttgccg aacgcttctg gcgtgaactg
    12481 cgcgagctgg agattgtgct tagctgacat tctcaagcgt cgcactgcgc caaccgccat
    12541 tatcccagac cagatcaaac gttagcggtt catcgtcgtg gtacagcatt atctcaaggc
    12601 tgatcgccag agtgtggcag tacgcttctt tcaaattaac ctgcaacgcc gcaatccgtg
    12661 gctcatgaaa taacagtgca gaacgcagct gacggcaata ccagctctgg agatgtttgt
    12721 cgctcgggtt atgcacttgc cactcgggca tgcctgaaac aagtaatgac ggtaatgtcg
    12781 aatgaggaga attaaaaaga gtgaggagat cgctgcatac cgatttacgc agcaatgacg
    12841 ttttattgcc tgaaatttca ggtaaaacag cctgaagtga ttggcgcatc attttgcgca
    12901 ctcctgacgt ttagcaacat atgcggcttg ccctgaacac cgggcaccac tgtattgatg
    12961 ctcattttcc atagccattc ccttttgttc attacatcca tgtcagtctg agacaggcct
    13021 gaatcctgtc agcaaagtat aaaaccttac ttgcctatga atatctactg acttttcagg
    13081 aaaaaagtca tgatttgata aaaatcatca aactcaaaaa aaaaccgcta gtatattctg
    13141 aatattccga atagttacgt agatgaaaat acttgtcacc gctgctactt aattccggtg
    13201 ggaa
//
LOCUS       DAZKIF010000124        13059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZKIF010000124 DAZKIF010000000
VERSION     DAZKIF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.125"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(174..1628)
                     /gene="amn"
                     /locus_tag="ACHOTW_003754"
     CDS             complement(174..1628)
                     /gene="amn"
                     /locus_tag="ACHOTW_003754"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP9845779.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(1730..3046)
                     /gene="shiA"
                     /locus_tag="ACHOTW_003755"
     CDS             complement(1730..3046)
                     /gene="shiA"
                     /locus_tag="ACHOTW_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP9845780.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            3360..4412
                     /locus_tag="ACHOTW_003756"
     CDS             3360..4412
                     /locus_tag="ACHOTW_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP9845781.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            complement(4674..12656)
                     /locus_tag="ACHOTW_003757"
     CDS             complement(4674..12656)
                     /locus_tag="ACHOTW_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFP9845782.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEISLAASKDPVIANNNDLTTLTATV
                     ADTEGNAIANSEVTFTLPEDVRANFTLGDGGKVVTDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNAPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVLVNGANLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSKWER
                     ITATPKTGGPSYIYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPLGDHLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFHSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(12970..13045)
                     /locus_tag="ACHOTW_003758"
     tRNA            complement(12970..13045)
                     /locus_tag="ACHOTW_003758"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13010..13012),aa:Asn,seq:gtt)
BASE COUNT         3225 a         3140 c         3170 g         3524 t
ORIGIN      
        1 tttataattg tgttttgaaa gcatacttgc ttttaatatg tggaaatcat taagacgata
       61 ataaaactcg tatccgccca aggaagcaaa cagcccgcgt ttattgcggg ctatttgcca
      121 gatgtcttac ttaatccttc acatctattt ttgataagaa ggttcagaac ttattatcgg
      181 aacggcggct cattaaaggt tcgtaattta cgtgaatgca gtcggtcgcc ttctgcgcgc
      241 agcaaatcga tcgcccgaat gccaatttgt aggtgttcgg aaatagcgcc ttcataaaaa
      301 cggttagcct gaccgggaag tttaatctcg ccatgcaacg gtttatctga aacacacagt
      361 agtgtcccgt atggcacgcg gaaacgatat ccttgcgcgg caatggttgc actttccata
      421 tcaattgcta cggcccggct taagttaaaa cgaagtgcag aagctgagta acgtaattcc
      481 cagttcctgt catctgtggt taccacagta ccagtacgta gccgctgttt gacttcctca
      541 ccgggcctgc cgctcaccag cttggtggcg tcataaagcg cacgttgcac ttcagcaatg
      601 ctcggaatag gaatatcggg tggcagaacc gcatcaagaa cgtggtcatc gcgtaaataa
      661 gcgtgtgcaa gtacataatc gccaatggcc tgactttcac gtaatccgcc acagtgacca
      721 atcatcaacc agacatccgg gcgtagcact gccagatgat cgcagatggt tttagcattt
      781 gacggtccca cgccaatatt caccagagta ataccctgac catcggcggt aattaaatgc
      841 catgctggca tctggtgttt tttccatgca aggtcggaaa tggcttcttc tggcgcttcg
      901 gtttcggcgg tgatccagtt cccgccagca caagaaaggg caatgtaggg actatcagga
      961 tcgaggatct ggctgcatcc ccaacgaacg aattcatcca cataacgtgt gtagttggta
     1021 aacaagacga acggctgaaa atgttcaact ggcgtaccgg tataatggcg caaccgcgcg
     1081 agggaaaaat cgacgcggcg cgcatcaaaa tgcgatagcg gggagaattc agttggatga
     1141 taaatgccgt ctgcagtttc atcgccaatt tgcgccagtt ctgttgtcgg gaaatagcga
     1201 gttaacccag cgctcattga gcgatcaagt gttaattcag agccatcgat gacataagga
     1261 taagggattt catgctgtga gggttgcacc gagatatgcg cgccataatc ctgatacagc
     1321 aacgtaagtt gttcattaag atacgaacga aagagagtag ggcgagtaat cgtggtggtg
     1381 tagctgcctg cgtgagtaaa acgaccaaat gcgcgcgttt tggggggatt tgttgtgcta
     1441 ccgtcccagg ttacggtcag agatggatag acaaaaagac cttgtttgcg ggcgttttca
     1501 tcaggtaatt cgccacttgt aatatagttg ccaatggcgt tgcgtaatgc gactacagat
     1561 tgctcataca gcgcgtcgag tttatccagt gcctgagctg gggtcagacc ggagccctta
     1621 ttattcatgt gtgtctcctg ttccatacaa ttaccgcaca tccgatagta tgtcacagga
     1681 atgtgaaaca aaagtgtaag gcgaagatcg gatgatcttc gccagactat caagcgcgtt
     1741 gattgtcttt catcaacaaa gcggtcattg cggaaatcag acatccagcc agcaaataaa
     1801 tggcgacgct atgccagttc ccggcaaagt aagtgatgag cgcagcggca ataaaaggtg
     1861 taaatccacc gccaaccaca ctggcaacct gataaccgac tccagcacca ctatagcgat
     1921 aactggcacc aaacatttcg gtaaacatcg gttgttgcac acacaccacc atgtcatgcg
     1981 caatgtttgc cagcattatg gagaagaaaa ctatccagaa aatagattgt gcttcaagcg
     2041 ccataaagaa aggaaatgcg ctcaacgttc cgatcaacgc gcctgtgata taaaccctgc
     2101 ggcgaccaaa acgatcggca agccaggcaa aacagggaat tgtcaggcag cttaatccac
     2161 ctaccagcaa accaatatta aggaaaagtt cgcgcggtaa ccccatattc tgagttgaat
     2221 aattaagtgc aaaggcggta acgatgtaca ttgtcagcaa ctcgcacagt cgtagcgcaa
     2281 taatcttcag gaaagcaccg ggatgtcgta acagcgcttc gataaccggg atgcgttttt
     2341 tcgcggccgc ttgattatat tgctgttgtt caaattccgc ggactcttcc atgccattgc
     2401 gcacccacaa tgctcccagt accagtacga tgctaaacag gaaaggaatg cgccagcccc
     2461 agcttaaaaa ctgttcgtca gtcgtcatca tactgatcaa tgaaaccagt ccggttgaaa
     2521 gcagtaaacc tacaccgtag ccaacttgta caccgctact gtaaaaggct tttttatttt
     2581 tcggtgcact ttcaacggaa agcaacgccg cgcctcccca ttcgccgccg acggcaaatc
     2641 cctgaatggc acgcagcgtc accagtaaaa taggtgccca ccacccaatg gtcgagaatg
     2701 aaggaagaat accaatcaag gctgtcgcga tgcccatcat ccagacggtc agcattaaca
     2761 tgcgcttacg tcccagtcgg tcgccaaagt gaccgaaaat gacaccgccg agcggacgga
     2821 aaaggaatcc gacgccaaag gtggcaaatg cggcgagcgt tcccatagcc gggcttactt
     2881 gcgggaaaaa ctcgcgatta aacaccagtg cggcggtaat gccatagagt aaaaaatcat
     2941 accagtcgac gacggcacca gcgaaactgc ctaacgcagc tcgtcgggcg cgacttaacg
     3001 aaaccgtcct ttcatcggga cgagtggaga tgagcgtgga gtccataatg atcctgtttg
     3061 tactgtcttt ttataattag tatggaaaag gtaactcaca ttgcgtcgat ctataaacga
     3121 accttatgag actacctcga cagagcgcat ctgcaaatga ttttatggct gattaacggc
     3181 aacatgaaaa attcgctgga agatgaaagc gcaggagttg ctggtgttgt ttaaggatta
     3241 agcagaggcc ccacaggttc gagatatgaa acataaaagt ccgcatatgc acaaaattgc
     3301 accctgatga ttctgataga ctgcattgca ttataaccag tagggaggcg aggttagtta
     3361 tgtttttagt atcattgttg agacgtattg catttagtta ctacgattat aaagcttata
     3421 atttcaatat tgaaaaaaca gactttgttg tcatccatat tcccgatcag attggcgatg
     3481 ctatggccat ctttcctgtt attcgggcgc ttgaattgca taaaattaag catctattaa
     3541 ttgttacgtc gacaattaat ttagaagtct ttaatgcgct caaacttgaa cagactaaat
     3601 taacattagt cacaatgact atgcaggatc acgcaacatt aaaagaaata aaagatttag
     3661 cgaagaacat aacacagcaa tacggtacgc cggatctttg tatagaggcg atgcgtaaaa
     3721 agaacctgaa aacgatgtta tttatcagtc agttgaaagc aaaaacgaat tttcaggttg
     3781 ttggtttaac catgaagtgc tattcccctg tgtgtaagaa cgcgtcccgg atggatcaga
     3841 aactccgggc tcccgtaccc atgacatggg catttatgat gcgtgaggcg ggttttccag
     3901 cagtcaggcc aatatatgaa ttgccactaa gtgaggatgt actcgatgag gtgcgcgagg
     3961 aaatgcgatc gttaggatct tacattgcgc ttaatttaga aggtagctcg caggatcgta
     4021 cattttcact atcgattgca gaaaatctaa tagaaaaaat tcaaagtgaa acagatatac
     4081 caatagtgat cgttcatgga ccaaaagggg aagataaagc cagggtatta gtggattgtt
     4141 ataataatgt ctaccgttta tccttatcac cctcgattaa acgttcagca gcaatcataa
     4201 aagatgctta tatcgcaata actcctgaca cctcaatatt acatatggca agtgcctata
     4261 atacccccgt tgttgcaatt tatgctgatt acaaaacgcg atggcccgca atggccgatg
     4321 tttcggagtc agtcgtcgtt gggcaaaaaa ttgacaatat aagtctggat gaattcgcaa
     4381 aggcattaaa aagtgttttg gcgagaatat gagacagtac ttatgcatag taatgccgat
     4441 ctgttatgaa cggtggtgtc atcaatactc tgaggctggt caatcatcac agaaaaccag
     4501 cctttggata aaagctgtac tcaccggata cgatcatagt cacatcgaac gcgattcttg
     4561 cggtatcgca tttttattta ttcattttct gtgagagaag ggcaagctct atagagggaa
     4621 tgtaaaaaag agtgccccga atggagcact ctttaagaaa ttataacgga aaatcagagg
     4681 tttttataac atgtcgcata agcaaaccca agcttttcaa atacgctttg atcacctgtt
     4741 gccagggaaa ctacgtattg ttcgtttgag tttgcgggac tcgatgacca atacatattt
     4801 gaatgaaaac cagcttcagt cgtgtaatgc cccatatctc cccattcact gtacagtgac
     4861 ccgatgcctc gggaacgact atggtttaaa tggtctccaa ggggaagtgt gtagccattg
     4921 ctactgcaaa aattttcagc aaggctgtat atcatgaaag catcgccggc gttcacccac
     4981 caactcttca ctcggatttc gtatatatag ctagggccgc ctgtttttgg cgtcgccgta
     5041 atcctctccc atttgctgcc gacattttta aatgtcactt taccggtagc atcaacatca
     5101 acccaggagg cagagcttga aaactcataa tcagccgccg tttttcctgg ggcaaagttg
     5161 tcattattca actgataata cgccccagtg aacccctgcg aagggaatgt agttgtcggt
     5221 aggttagcac cattgactaa cactgtaccg ctcattattt tgtcttctgc gcgagtgaat
     5281 tgtattgtgg tactcagacc cgcttgatga acaccattca atacagggat cagcgttgcg
     5341 ataccctctc cgccgcctgt aacagtggct ttgtactcgc ctgagaaatt tttactgtag
     5401 tcaattgcac taacttgcac atagggcgcg ttggtacctg actgcacaaa ttccagccct
     5461 tcagaaactt tgatcggatt gcctgatata tcgtgcagaa caagatgtag ctcagcacta
     5521 tcggtaaagt ctcctttcaa tgatgaccga ttgttcttaa ggacggactg cgaggcgtct
     5581 gccgggcctg ccaccagcgt tatatctact gtttgcattc caccagcggt cgtgactgcg
     5641 cttaccgtga cgctccccgt tatcgctcct ctcacgcttg ttgtcgcgat tccgttttga
     5701 tctgtcaccg ctgttaatga cgttaatgtg gcagagccgc tttttaaggc gaagtacaca
     5761 gtgagacctt cgatcaggtt accactgcca tcctcaaccg ttgcctttaa ggtacttaaa
     5821 tcactgttgg tggcggctat agtcgatgga tcagcgataa agctagcaac atgggcggtg
     5881 cttgagttgc cagtgacttt cacgattgtc tgtgtctgta ttgttacgcc gttatggaac
     5941 gatgcagtca ccgtatatgt accgacttta ttgctggtca gaacgactgg agcctgacct
     6001 gaagagttgg ttctcacttt tccgccactt aacgtcgcac cttctggcgt tacgctaaag
     6061 ttgatgacct gaccctccac tggagtgcca tttgcagaag ttagcgttgc cgtcagagtt
     6121 gcacctgttg gggaattgac accgataagc gggctggacg ccgagagtgt cagtttttga
     6181 tcgattacca ccagatcctt ctcataagag gatccattcg ccagggatgc tttcaccata
     6241 tacgaaccgt ttctttcggg cgtcatagtg acctcggcta taccatgcgt atcagtagag
     6301 acaatatttt ggctgatggt catgtgctct ggtggttctg cactgaatgt tacagattca
     6361 ttaagaatcg gattgccaaa ctggtcgttg acgtgagctt taactgctac gtcttgtgcg
     6421 accatgacct gaccttcagg tatctccaga ctggtaatcg ttgcagaatt aatatctgct
     6481 tttgcattca gtaatcgcga gatgacttca gtaggtttat ctgccagaga ggctgaaact
     6541 gttttcagtc cgacctcggt gcttgtcaca agaatttcag cgaaaccccg atcatccgtt
     6601 tcaacgctgg tgctgcttag cgtgacggaa gttccgcgga aattaacttt tataccttct
     6661 accgggttgc cgttagcgtc agtgacgctt gcagtcatgg tcgcgccctc ggtcgtgctg
     6721 acgacgaatg agtatacaga ggttaaggag gctagttttg cggtaccagc atcggcaatc
     6781 aaagtcacct gtttcgtatc actgacacca taattgttca cgctaactgt cacggggtag
     6841 gtgccggatt ttgtaccgct cagtgtaact tcagccttgc cgttgatgtc agtaatggcg
     6901 gaaccgcctt gtccgagagt aaagcttgcg ctcacatctg ccggtagcgt gaatgtcacc
     6961 gcctcattgg ctaacgggtt gccgtttcca tcagtcactg ttgcctgcag cctggtcatc
     7021 ccgacgttat tagcgataaa attgtcctcg gtaacgttaa gattaacctg cgcagtgagt
     7081 gtatccgcaa taaagttcac caccaactgc tcactcttac cgccagccat cgatgctgta
     7141 acagtatgag cgcctgcttt tgtacctttc agcgtgactt tcgctttgcc ttcagtatca
     7201 gtaaccactt taccgccatc gcccagcgtg aagttcgccc tcacatcttc cggcagagta
     7261 aatgttacct cactgttggc tatcgcattg ccctctgtat cagcgactgt tgctgttagt
     7321 gtcgtgagat cgttattgtt ggcaattacc ggatccttcg aggctgccag cgagatttca
     7381 gcattcgcta cgttcggcac ataggtcact gtttgttgca tcgaatcgcc attttcgagg
     7441 gttgccgtca cttggtatac cccggctttt gtagcggtaa agcttgcgta aaaattgccg
     7501 tcgtgattgg tcgtatatat agcgttatta ctgggggtca caccttcact tggtgaaacg
     7561 ctgagggtta cctcctgctg gggtactccg ttgccgtaat tatccttcac ctcaatatac
     7621 agattggtag tgtcgcctgc ggagactgta tcaaaaagtg cctgtagcaa ggtgagatgt
     7681 gccgtagacg catcagcgtt gacattaatt gatgtgctaa gcgtggagct accattttcc
     7741 agactggcct caacggtatc cggtctcgct cctgattcta tcgaggagcg ggtattggta
     7801 taagtgacgg tagccttacc ctgttcgttc gtcacggctt gaccgccatt cgtcatttcg
     7861 gctgtcgctg cgttgctggt gaagttcaca gtcacacctt tcaccggaaa gccattatta
     7921 tcaacgactg tggcggtgat gacgctgccg gagctgttct gcggggtacc tgcgattatg
     7981 ctgtctggga caggcgtcaa ctcgataatt tttgccgccg ctgtgtcgcc aataaaatgc
     8041 acagttttgt tgtcgctggc accattatta gccagtgatg cagtgaccgt ctgctcacca
     8101 aaggcaacgc ctgcgagagt ggcctgcgcg atgccagact cattggtatt actttcccct
     8161 ggggtcaggg tgaggcctga agaagctgtg ctgaatgtta ccggaagatt gttcacctca
     8221 ttgtcgaact gatctttgac cgttgcagtt agcgttgcgc tatcgacgcc attacctgtg
     8281 atctcatttt ttgatatctg aagaacaacc agagccgagg ttttgtccgc cacaaacgtt
     8341 accggctgtg aatcactggt attgttatta ctcagcgttg cggtaaccgt atgcgtaccc
     8401 gcttttttac ctttgagcgt gacatgcgct tccccattgg cctgggtgat ggcaataccg
     8461 ttattttcga gggtaaagtt tgccgccacg tcctgtggca tggtgaagtt caccgttatt
     8521 cccgccaccg gatgattcga cggatctttc accgtcgccg tcagctgcac gctgtcgctg
     8581 ttattcgtca ccacctgttg agcagggatg ttcagcgtaa cctgcgcgtt gctggcatcg
     8641 ggcgcaatat tgactatctt cgtatcgttg ttgccgttag gcagtgtggc ttcggctgta
     8701 tgaacaccca aaaccgtgcc cttaaaggta acttcggcca caccactgtc gttagtattg
     8761 cttttgctct gactaagttg agtatctgcg gggttggtac tgaaggtgac ctgtagatct
     8821 tttactgcgt tatcaaaagg atctttcact gttgccgtca gagtcgtctc atccacgcca
     8881 ttcccaatga tttccgcttt cgatgtttgc agaacgacaa ccgcgctgtc cttatccgcc
     8941 acgaaggtga ctggttgcgc atcgctggca ttattgttac ccagcgtggc ggtcacagta
     9001 tgcgtgcccg cttttttgcc tttgagggtg acatgcgctt cgccattggc ctgagtgatg
     9061 gcaataccgt tattttcaag ggtaaagttt gccgccacgt cctgtggcat ggtgaagttc
     9121 accgttattc ccgccaccgg atgattcgac gggtctttca ccgtcgccgt cagctgcacg
     9181 ctgtcactgt tattcgtcac cacctgttgt gcagggatgt tcagggtgac ctgcgcgttg
     9241 ctggcatcgg gcgcaatatt gacgattttc gtatctctgt tgccgttaag cagtatggct
     9301 tcggctgtat gaacacccaa aaccgtgccc ttaagggtaa cttcggcaat accgttctca
     9361 ttagtattac gcgcgttcag actcagttgc gtgtctgcgg gggaggtgcg gaagactact
     9421 gaaagatttt taaccacgtt atcaaaagga tctttaactg ttgctgtcag agtcgtctca
     9481 tccacgccat tcccaatgat ttccgctttc gatgtttgca gaacgacaac cgctctgtct
     9541 ttatccgcca caaacgtcat tggctgtgta tcgctgacat tgctgttagc cagacgagcc
     9601 gtgatcatat gcgtgcccgc taacgtgccg gtcagggagg cgattgcagt cccgttgcta
     9661 tccgtcatgc cttttccgct gttgctaatc gagaaccgac ttgcgacgtc gtttggcaca
     9721 gagaaggtga tttctttatc ttttagtgga ttgccatttt tatcctgcaa ggttgcagtc
     9781 atatgtagcg gagctgtgtt ggtgacggtg atatcacctg aaggcacact gagggtcagg
     9841 gcagcagtac tttgatcacc gataaaaatc acctgttgat tagcctgaga accagagctc
     9901 acagaggccg taaccgtata atcaccattt ttcaaactgg tcagcgtagc tgtggcgatc
     9961 ccgtcgtggc tattcacttc ggtttggctc agtttcgctg ctgctgaatt gacattgaag
    10021 gtgaccttga cgtcattgag caggttgcct tttgcatccc gaactgtcgc ggtcattgtg
    10081 gcactgtcat tgccgtcagc gaccacttca tttttcgact tctgcagatc aacctgcgcg
    10141 gtactcgagt cgccgacaaa actgacgatt aacgtttgtt tcacgccatt ttcaagggtg
    10201 acttcaaccg tgttgtcttc ctgcttacta cttttcagat caaaagtcgc cagaccatta
    10261 acatccgttt ttgcggtgtt ttgattgtta aaggaagttg ccgatccgct taataccgca
    10321 aacgtgacgg tatgatcatt gattgggttg cttccttcat cagcgacatt gaccgagacg
    10381 gtgtttgctg cattctcatt ggcgagcaca ccattattgc tggcagataa tgtcgcaatt
    10441 ttcgctgact gcgggttggc gtcgatgata aatccagcgg tatgcaaatc ttcattccag
    10501 ttttgcatta acagcttcgc agtaagccca ctgcctttgg tataggcggt ataggttgcc
    10561 ttatagacgc catctgcggt ttctttccag tctgtagtga caccaggttt cacgttgtcg
    10621 atgctgactg cggtattcag ttgctgtttt tgctccttaa caggtttgtc attttcatct
    10681 ctcagttcta ccgtcacctc gataggattc ccggagagat aacggtcctt atcaatctta
    10741 attgacgagt gagtccggga tgacgaaaca gaaatgatat tcaccacggc gggggcttta
    10801 gccgcatcca caccgttcag ctgtggcatc agcgtcagcg tgccagacat cgctcctgtg
    10861 gtcaggatct gggtatagct tccgtcacca ttatctttcc agtcagaaag ggtgatgtcc
    10921 tgaacacctt cgtgacgcgt cgagagcacc agcccgataa caggattacc tgctgcatca
    10981 tgcgcaataa aagtcagtgt ggcggttgaa tgggaatccg cgctcaacgt ctggctactt
    11041 aacgataccg aggaatcttt ctggcttagc gtaggagcct gaacgactac catgctctgt
    11101 tcacgattcg aaaaattgcc tttgacatct tcagcggtga cttcaatcgg ccaggtgtta
    11161 tcggtttctg gcgtactggt gaaccggtac gccggcaggg taaccagaat atctttaccc
    11221 gttgtaacca ctttaccacc ggcagcttcc agagcggtgg cttcgacgtt atagcctttc
    11281 agggcatatt tggtttgtag cgacgaaacc agtgatttca cttctcctga cttccctgtc
    11341 acggggtctg tcagggtcag gcgaaccagt tcttttttgc gatattccag aacgatgttg
    11401 ttgttgcgat ccaccagatc aaaacggctg cctgcaaggc tacgccgtgc atcgacttca
    11461 ttcgggtcaa gctgtttctg catcgcgctt ccaggttgcc aggtaaaatc gacggcaaaa
    11521 cgggtgtcat tttcgccctg tttaccctgg cgttgctccg cgctgaaggt catcagcggg
    11581 aagggggtat agttaagtcc agcggttatg gcatgaggat tactttgccg atcatcttta
    11641 tcgaacaggg ccacttcatc gccataatac tgttcataga ccagtttacc gccaaggtgc
    11701 ggccaggcgg gtagccagcc ttctgcgcgt acatcccagc cattggccgg gcgtgcttca
    11761 taatcgttgt ccagttcagg tgcgctgcgc cagttggtca gtcgcaaata gccgttactg
    11821 cttaatttta gatagtcgcg ccagtactcc gcgccaatgc cggcgcggga gtggtaacgg
    11881 ctaagatcgt ggtcgaaaaa gaagttgatg cccgacatcc atgtgggagt gaaatgacgc
    11941 caacccaagc cgttgttaat ctgcgtacgc tcgtcagtac gatggagagt atgctgactg
    12001 aaaaagagat tatcaggcgt ttcataccac ggatggagaa aatcgaactg ggagttcttc
    12061 aggctaaaat cttcatccac gcccagcgtg attcttgcgg taccgaagcg gcttaaccag
    12121 tctgtcattg cgcctgaagc ctgagaagag gcccatccac gcgccatatt tgccgcttgc
    12181 tcgctgttca tatcctcggc gagcagagac ccgatttgct gtgaagtact ggctatctgt
    12241 tgctcaaggt tgccactgct attacccggt ggcggggtta aattattttc actaacttgt
    12301 gccgggacat ccagttcatc accctggcgg acattatcaa aacctcgagc aaacgtacga
    12361 aactggttga gtttgcgtaa ctcagccacc gaaataccga aacgttcggc aacgctttgg
    12421 gccgattcca gcgctccaag ggtgtagggc accgtattgg catttgcaat ggcaatttgt
    12481 gcaggaactg gttgttgggt tgcggcgttt accacacctt gtgctgccgc agccataggg
    12541 aacacaagtt gagttatcag gcagataccc gcagttaagc ggcgtagttt aattcccatc
    12601 ccgcataaga tttgtcggtc atttatttct tctccacttc tcttcttcgt agccatgctt
    12661 accttccctg aacgagctaa cataaatgcg ccctttctag cacagcgaaa aaatgtaaat
    12721 cctgatagca atatgattga aaattaataa cttttcgtta ggcagttttg gatgtgagtt
    12781 gcaagagggg agactactga ataactcaag ttttataatc gaggggaaaa tggtgatggc
    12841 gttcatagca aaacgccctc aaccataaag gtcgagggcg cttaagatgt taaaaacccg
    12901 ctatccgtta aaaaacaatg ttcaactaag gtcagtgaca ttgcgctaaa aaagcgaatt
    12961 tttagaattt ggctcctctg actggactcg aaccagtgac atacggatta acagtccgcc
    13021 gttctaccga ctgaactaca gaggaatcgt gtgaacggg
//
LOCUS       DAZKIF010000125        12432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZKIF010000125 DAZKIF010000000
VERSION     DAZKIF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.126"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(181..444)
                     /gene="cedA"
                     /locus_tag="ACHOTW_003759"
     CDS             complement(181..444)
                     /gene="cedA"
                     /locus_tag="ACHOTW_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP9845783.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            627..2888
                     /gene="katE"
                     /locus_tag="ACHOTW_003760"
     CDS             627..2888
                     /gene="katE"
                     /locus_tag="ACHOTW_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP9845784.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2935..3693)
                     /gene="chbG"
                     /locus_tag="ACHOTW_003761"
     CDS             complement(2935..3693)
                     /gene="chbG"
                     /locus_tag="ACHOTW_003761"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP9845785.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3706..5058)
                     /gene="celF"
                     /locus_tag="ACHOTW_003762"
                     /gene_synonym="chbF"
     CDS             complement(3706..5058)
                     /gene="celF"
                     /locus_tag="ACHOTW_003762"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9845786.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5164..6006)
                     /gene="chbR"
                     /locus_tag="ACHOTW_003763"
     CDS             complement(5164..6006)
                     /gene="chbR"
                     /locus_tag="ACHOTW_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP9845787.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6014..6364)
                     /gene="chbA"
                     /locus_tag="ACHOTW_003764"
     CDS             complement(6014..6364)
                     /gene="chbA"
                     /locus_tag="ACHOTW_003764"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP9845788.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6415..7773)
                     /gene="chbC"
                     /locus_tag="ACHOTW_003765"
     CDS             complement(6415..7773)
                     /gene="chbC"
                     /locus_tag="ACHOTW_003765"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP9845789.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7858..8178)
                     /gene="chbB"
                     /locus_tag="ACHOTW_003766"
     CDS             complement(7858..8178)
                     /gene="chbB"
                     /locus_tag="ACHOTW_003766"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP9845790.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8478..8816)
                     /gene="osmE"
                     /locus_tag="ACHOTW_003767"
     CDS             complement(8478..8816)
                     /gene="osmE"
                     /locus_tag="ACHOTW_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP9845791.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9018..9845
                     /gene="nadE"
                     /locus_tag="ACHOTW_003768"
     CDS             9018..9845
                     /gene="nadE"
                     /locus_tag="ACHOTW_003768"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9845792.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10075..10962
                     /gene="cho"
                     /locus_tag="ACHOTW_003769"
     CDS             10075..10962
                     /gene="cho"
                     /locus_tag="ACHOTW_003769"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP9845793.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10922..11497)
                     /gene="ves"
                     /locus_tag="ACHOTW_003770"
     CDS             complement(10922..11497)
                     /gene="ves"
                     /locus_tag="ACHOTW_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP9845794.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11700..12185)
                     /gene="spy"
                     /locus_tag="ACHOTW_003771"
     CDS             complement(11700..12185)
                     /gene="spy"
                     /locus_tag="ACHOTW_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP9845795.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         3292 a         3140 c         2837 g         3163 t
ORIGIN      
        1 atgattagca aaacttaacc attttaaaat aaataaacaa ttaaagaaaa aagatcactt
       61 atttatagca atagatcgtc aaaggcagct ttttgttaca ggtggtttga atgaatgtag
      121 caatgaaata gagaatttca ggccatgtaa ctcccggcaa aaccgggagg tacgtaatct
      181 ttactcagtc acttcccctt cctggcggat ctgatttgcc caacgctggg cggattcagg
      241 cacagtaaac gccggtgagc gcagaaatga ctctcccatc agtacaaacg caacatattt
      301 gccacgcagc atccagacat cacgaaacga atccatcttt atcgcatgtt ctggcggcgc
      361 gggttccgtg cgtgggacat agctaataat ctggcggttt tgctggcgga gcggtttctt
      421 cattactggc ttcactaaac gcatattaaa aatcagaaaa actgtagttt agccgattta
      481 gcccctgtac gtcccgcttt gcgtgtattt cataacaccg tttccagaat agtctccgaa
      541 gcggggtctg gctggtggtc tatagttaga gagttttttg accaaaacag cggccctttc
      601 agtaataaat taaggagacg agttcaatgt cgcaacaaaa cgaaaagaac ccacatcagc
      661 accagtcacc actacacgat tccagcgaag cgaaaccggg gatggactca ctggcacctg
      721 aggacggctc tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa cctaccgcct
      781 cagggagcct gaaagcccct gatacgcgta acgaaaaact taattctctg gaagacgtac
      841 gcaaaggcag tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc gccgacgatc
      901 aaaactcact gcgtgccggt agccgtggtc caacgctgct ggaagatttt attctgcgcg
      961 agaaaatcac ccactttgac catgagcgca tcccggaacg tattgttcat gcacgtggat
     1021 cagccgctca cggttatttc cagccatata aaagcttaag cgatatcacc aaagcggatt
     1081 tcctctcaga tccgaacaaa atcaccccag tatttgttag gttctctacc gttcagggtg
     1141 gtgctggctc tgccgatacc gtgcgtgata tccgtggctt tgccaccaag ttctatactg
     1201 aagagggtat ttttgacctc gttggcaata acacgccaat cttctttatc caggatgcgc
     1261 ataaattccc cgattttgtt catgcggtaa aaccagaacc acactgggca attccacaag
     1321 ggcaaagcgc ccacgatact ttctgggatt atgtttctct gcaacctgaa actctgcaca
     1381 acgtgatgtg ggcgatgtcg gatcgcggca tcccccgcag ttaccgcacc atggaaggct
     1441 ttggtattca taccttccgc ctgattaatg ccgaagggaa ggcaacgttt gtacgtttcc
     1501 actggaaacc actggcaggt aaagcctcac tcgtttggga tgaagcacaa aaactaaccg
     1561 gacgtgaccc ggacttccac cgccgcgagt tgtgggaagc cattgaagca ggcgattttc
     1621 cggaatacga actgggcttc cagttgattc ctgaagaaga tgaattcaag ttcgacttcg
     1681 atcttctcga tccaactaaa cttatcccgg aagaactggt gcccgttcag cgtgtcggca
     1741 aaatggtgct caatcgcaat ccggataact tctttgccga aaacgaacaa gcagcattcc
     1801 atccaggtca tattgttcct ggtctggact tcaccaacga tcccctgttg cagggacgtt
     1861 tgttctccta taccgataca caaatcagtc gtcttggtgg gccgaatttc catgagattc
     1921 cgattaaccg cccaacctgc ccttaccata atttccagcg tgacggcatg catcgtatgg
     1981 ggatcgacac taacccggcg aattacgaac cgaactcgat caacgataac tggccgcgcg
     2041 aaacaccgcc gggaccgaaa cgcggcggtt ttgaatcata tcaggaacgc gtggaaggaa
     2101 ataaagttcg cgagcgcagt ccatcttttg gcgaatatta ttcccatccg cgtctgttct
     2161 ggctaagtca gacgccattc gagcagcgcc atattgtcga tggtttcagt tttgagttaa
     2221 gcaaagtcgt tcgtccgtat attcgtgagc gcgttgttga ccagctggcg catattgatc
     2281 tcactctggc ccaggcggtg gcgaaaaatc tcggtatcga actgactgac gaccagctga
     2341 atatcacccc acctccggac gtcaacggtc tgaaaaagga tccatcctta agtttgtacg
     2401 ccattcctga cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat gatgaagtga
     2461 gatcggcaga ccttctggcc attctcaagg cgctgaaggc caaaggcgtt catgccaaac
     2521 tgctctactc ccgaatgggt gaagtgactg cggatgacgg tacggtgttg cctatagccg
     2581 ctacctttgc cggtgcgcct tcgctgacgg tcgatgcggt cattgtccct tgcggcaata
     2641 tcgcggatat cgctgacaac ggcgatgcca actactacct gatggaagcc tacaaacacc
     2701 ttaaaccgat tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc aaggtcgctg
     2761 accagggtga agaagggatt gtggaagctg acagcgccga cggtagtttt atggatgaac
     2821 tgttaacgct gatggcagca caccgcgtgt ggtcacgcat tcctaagatt gacaaaattc
     2881 cggcgtaaat atggataagg tcacgtcccc tggggagtga ccttaccacc ttacttacac
     2941 atcaagataa ctacccaacc gataaccacg ctcagcaatt gcatatttca acgacgctga
     3001 tgtcagcact tccagctccg ttaaccgtgg gaaacagtaa gcactctggc gaatcgtatt
     3061 atcgacaaac gccggatggc acatgacttc cagcgagcgt tccccgcgat ggctggaatc
     3121 atcaagcact tgcaggaaca gtgcctcgct aatctcttca ccataaaatg cgctactgaa
     3181 tccgtgcgaa ctgcgcagat tagccggtaa atcaccagcg ttagatagcg gctgacggtc
     3241 gatgcgcaac gcaatcccct cttcagccgc aaacctggca acaataggga aaatctgcgg
     3301 gaacatatgc acatgatgat ggctatcaag atgcgtaggt ttgcgtccaa atagctcaat
     3361 gaaacgcaaa tactgactgg caagctcctg agtaatttct tccagcggta aagcatcttc
     3421 ttctgccaac tgccagatcc attttcccag cacaccatcg cgggttaacc ccggcatagc
     3481 tgtcagtggc ttgcccatag taaggacaaa gttcatccct atggccagac tcggttcatc
     3541 acgactcaac tgcaccgcat ggtcaatagc ctgcccattc accagcgccg tcgtcgatgt
     3601 gacaatccca ttgcgacagg cctcgataat gccgtagttc tgacctttgc ttaagccaaa
     3661 atcatcggca ttaacaatca gtaagcgttc cataatcagc ctcggttaat gtgctttttt
     3721 aagctctgcg atgcagtcgg caaaatttgg cagccatttc tcgtgcgcca gaatcatctc
     3781 gcgtgccagc agctcagcat cgcgatcgga atgcaccaac ggactaaggt ttagcgccag
     3841 taacacatcg ttaaattctc cgctaagtgc ggcgttgctg gcagcaatct cgaagccttt
     3901 aatggtgtga atcagcccca tcactttatc atcgaaatgc gtaatgcgcg gatgtggcgt
     3961 cgcgccatcg cgccccagcg tacaggtcat ttctaccgcc cagtctgccg gaatattatc
     4021 aatatgccca tgatgcggga tattaacgta atgttctgct tgcttatcgt tgtagatagc
     4081 gttgatcact tcgcacgctg catcagagta ataagcccca ccgcgctgtt ccagttcttt
     4141 cggcttaact ttcaactccg ggtttttata cagctcaaaa agttgtttct cgactttctg
     4201 tactacctgc gctcgtgcgc cgcctttgta gtattcgccc atttcaatag ccagcatctc
     4261 tttttgcttg aagtaataaa gcaaataaga acacggcaac agattcagag aacgaattaa
     4321 gccttcgcta aatggcagat cgaaaatatt tttaacgcca gatgctttta actgcccgga
     4381 tgccacacca tcaagcaatt cggcaaagcg cgacttgcca tttaccagca catccttaat
     4441 gaacaccata tggttgaggc cgaacagatc gatagataaa tcatcgctgt ctttcagcat
     4501 cagaacatcg cgaataaaca tcttcatgcc gatcggaata ttacacacgc cgataaagcg
     4561 tttaaatccg gtatgacgat aaacggcttc agtgaccatt cccgccgggt tagtgaagtt
     4621 aatcacccat gcattcggac aaagttcttc gacatctttt acgatgtcaa aaatcaccgg
     4681 aatggtacgc agacctttaa acagaccgcc cgcgccgttg gtttcctgac caagataacc
     4741 atgacttaat ggaatacgtt catccagttc acgcgccggt aattggccaa cgcgcagttg
     4801 ggtagtaacg aaatcagcat ctttcaatgc ttcgcggcga tccagcgttt tataaagctt
     4861 catcgggacg ccagcgttat caatcatccg ttggcagaga tcgaaaataa tatccagttt
     4921 agctttgcca ccttcgacat ccaccagcca taattcgctg accggcaatt cgtgataacg
     4981 cttaataaat ccttccagta actccggggt atagctgctc ccgccaccaa tagtgacgac
     5041 ttttaatttc tggctcataa tttctccctt cagtacagaa tactgatatc tggcatatct
     5101 gccccccccg gacataaata atccagcaac aggacagata tgtgaattgt caggtataac
     5161 gacttactga ttaaattcag tcaatttctt acgatagctc ttaggcgtaa aggacgttaa
     5221 tttcttaaac gttttaataa acaaactcgg gctactataa ccggcctcaa acgcaatatc
     5281 cgtcactgaa tagttggtca tttccagttg ttttttggca aaattaatac ggatttcatt
     5341 aataatctgc attggcgttt tgccataata tcgttgagtc gcccgcgtca aatattcctg
     5401 tgatttggct gacaacgtca ccatattctc cagcgccgat tcactaaact gctctttatc
     5461 atgcatcttt tctaccgtac ttttcagcca ctgcggtaca tcatcaatca cctgttcttc
     5521 gcggtaatga cgtaaacggt taatgacata aaaagtaacc atctcaacaa actcttccag
     5581 ccctgtttcg cggaaattca atgaagaaat cactgtttcc acataggtga gaaaagcgtt
     5641 attggtccgg tatacctgcg aagctacaaa gcaataaggc aacaatggca ggtaatgctg
     5701 ctcaaaaaag cgtttactga tcccaacgtt caatatgcgc gtggcaccaa actcataaaa
     5761 actttgatgg tgcgaaccta acggaataaa aacaaaatcg ccccgttcca gtaacacgcg
     5821 cttaccgtta atctcctgga aataacgccc ggttaatacc agagtaaatt cataatagtc
     5881 gtgctgatgc agtccgctga tactctcagt tttgttatag ataaacacat ggaagttttt
     5941 gccattaaac aactgctgtt ctcgggcagt ggcaatttcc ggcgcgttaa tcactggctg
     6001 catcatcgac tccttatgcc ttcagttttt catgaagctc aattaattca gtaatcagtt
     6061 cacgcgcaag catggacgtc attaaatgat cctgagcgtg gaccagcacc agactcactt
     6121 tcatcttacc ttcgcccgca tcgccttcaa tcagtttcgt ctgtaccaga tgcgcttcat
     6181 tcaatgccat ccgtgactga tccatcatgg cttttgctgc ggcaaaatcg ccctgcttcg
     6241 cctgtttcag tgcggcatac gccaagctgc gcgcttgtcc ggagttgatg ataagcccca
     6301 tcaccacttc ttccagctct tcagcttccg tttgcgtatc gggaatatta tcgagatcca
     6361 tcatacatcg ttcctctctt ttcttaccgg cacgattacc cgtaccggca tcgattaaaa
     6421 tttcagggcg ttagcgatat cttcttcgct ctcttcttta tcaatcgcat tttgtgcttt
     6481 gttagccacc acaacaaagg gcagataaat taacgttgcg ataccaaggt tgaagagtgc
     6541 aaccagcaat gcgacgacgc taccgttggt gttaaagaag gctcccagac cggttggcat
     6601 ggtccacggt gcaatattgg tcaccggagg aataatgccc atgtagtacg ctgccagggt
     6661 gattgccgcc agaatcggtt gtaccagtac aaacgggata aacatcaccg ggttcatgat
     6721 aattggcaga ccaaacagaa tcggttcgtt aatctggaag atgccggacg gcagcgccag
     6781 ttttgccacc tgacgataat cagcacggcg agaggcaata aagatagcca ggatcaggcc
     6841 taaagtcgca ccactgcctc caaggaaaat aaaggagtcc agcatcggct tggcccagat
     6901 atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa ccatattgct gataggtcgc
     6961 gatattttcc agcgcccacg gcgtcataat gccgttgtcc agtgcggtca gcgccagcgc
     7021 gccatgaata ccgaagaacc agagcagtgg aacaaagatc acataggccc agcccaccac
     7081 gctacccaac gatgccagtg gggttgagat ggtatccata atgatctgat ggaagttggt
     7141 gccccaggta ttcaacgccc aggcaataat ccccatcacg gaaagaataa taaagccggg
     7201 aattaatgcc gagaaggaac gcgatactga agcaggtacg ctgtcgggca gtttaatgac
     7261 ccaattgcgg cggacaataa aggtaaacat ttctgccacc accaggccaa taataatccc
     7321 ggagatgata ttcgccccac ctaaccagtt tgcgccaacc gcataggcct caccgacact
     7381 atatggggtg acggtcataa atgctgcaac ggataacaac ccagccgcca gcgcatcgac
     7441 tttacgctct tctgccagcg ccatgccaat aaagaacggt gccattaaag acattatccc
     7501 taatgttccg ttatatacgt tgccgccaat acctttcaga ccattaagtg tttcaatggt
     7561 tgaggcgtcg aggcgaatac ctaaggaata aaaaaacgcc ccctccccaa agcttagaaa
     7621 aacgttgtta attaatacaa acatggcccc cgcaagggtt aacggcatta agcgaataaa
     7681 gccatttttg attgcattaa cgtgtggctg ctttcctatt ttaactgcaa aagggaggag
     7741 taccttttca agcgatgcaa taacattact catagaaaaa tacccttaaa aaccgcaatt
     7801 taaatattgc ggtattgatt tatgaaataa ctctttgacg ggaaaattta aaataaatta
     7861 atttgctgcg gcttttttaa tcgctgcaac cgcagcctta agcacgccta aaccatcgac
     7921 tttgccataa agcagcgagt caattacttc aaccggtttg ttgggtaaca aacgctggat
     7981 ttcgggcaac atataagcaa tctgcggccc taataacacg acatcggcat tctgaccttt
     8041 ttcaccagcc agtgtttccg gaaatgcttc aataatgacc ggaacttcat atttttctgc
     8101 ctgtgcgcgc atttttgata ccagtaaaga ggtagacatg cccgcagaac aaaacagata
     8161 aatgtgtttc ttttccataa aactgccctc atcgacgatt atctgtcagc cagacactcc
     8221 gcaagcctta acctgcttcc atgctctggg taacttgcga aaccaacatg actttttttg
     8281 tggctgaaag gagtatacgg tatagacctg agcaatagta tttctttgac cttataaatt
     8341 gtctctcctt gacctgcggt tatgaccctc ttcacatttc gggcaaataa ttcgcgaaga
     8401 taattaagaa ctcaggccaa aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa
     8461 gctttcagac gaagtgatta cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc
     8521 tgataaccgg agttgatgac atgtccggta tcatctaacg cgacaaagta ggtttctgct
     8581 ttaccatcac gttgacccag gatgtaggtc tggcaagtac cacgagcatg gatcatgctc
     8641 acttcagacg aaggtttacc cgcaatttgt gcaacctgcg cccggctcat gccttttttg
     8701 acgtctttca ccacaggctg tacaaactgg tctttggtac gatcataagc cgtacaaccc
     8761 gccagcatgg ttaataccgc cgctgcactc agaattcctg ccatattctt gttcatattc
     8821 cgtcctattg tttatcagcg tgttagataa gcctggaata cattgggcgc tttttcaagc
     8881 ccatgaacga aacggctccg ctttcagagg attcctgtat gacgttttaa ccaccattca
     8941 gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct ttaaggaagt tttgtctttt
     9001 ctgtctggag gggttcaatg acattgcaac aacaaataat aaaggcgctg ggcgcaaaac
     9061 cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga ttttctgaaa agctacctgc
     9121 aaacttatcc gttcattaaa tcactggtgc tcgggatcag cggcggtcag gactccacgc
     9181 ttgccggtaa gctgtgccag atggcgatta atgagctgcg ccaggaaacc ggcaacgaat
     9241 cactgcaatt tattgccgta cgcctgccct atggtgttca ggccgacgaa caagattgcc
     9301 aggatgccat tgcctttatt caaccggatc gcgtattaac cgttaatatc aagggcgcgg
     9361 tattggctag cgagcaggca ttgcgggaag catgcattga actgagcgat tttgtccgtg
     9421 gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag cattgcgggt atgaccagcg
     9481 gtgtcgtggt gggcaccgat catgcagcag aagccattac cggattcttc actaaatatg
     9541 gtgacggcgg tacggacatt aatccactgt atcgtctcaa caaacgtcag ggtaaacagt
     9601 tactggcagc attaggttgc ccggaacacc tttataagaa agcgccaacg gccgatctgg
     9661 aagatgatcg cccttctctg ccagatgaag tggcactcgg cgtgacctat gacaatatcg
     9721 acgactatct ggaagggaaa aacgtacctc aacaggtcgc cagaacaata gagaactggt
     9781 atctgaaaac cgaacataaa cgccgtccgc caattaccgt tttcgatgat ttctggaaaa
     9841 agtaataatt tgcacctatt ggattgtgca aaaaagagta atttgttcac gccggatgcg
     9901 gcgtgaacgc cttattcgac ctacaaaact atgcaaattc aatacattgc aggagtcgaa
     9961 taggcctgac aggcgtagca cgtcagacgg tgtaaccttt gtcatcgacc cgcctctttt
    10021 ttaatcgctt cccggctgtt acactggata gataaccagc attcggagtc aacagtggta
    10081 cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg caatttatga atatcccgaa
    10141 catttacgtt cattccttaa tgacttaccc acccgaccag gggtgtatct gtttcatggc
    10201 gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta atatccgcag tcgggtgctt
    10261 tctcatttac gcaccccgga tgaagccgcc atgctacggc aatcccggcg aattagctgg
    10321 atctgcaccg caggcgaaat cggcgctctg ctccttgaag cgcgattaat caaagaacaa
    10381 cagccgctgt ttaataaacg gttgcgccgc aatcgccagc tctgtgccct gcaattaaat
    10441 gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt tttcacgagc ccccaacctg
    10501 tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat tgcagagcat cgctgatgaa
    10561 caaaaacttt gttatggtct gttgggactg gaaccgttaa gtcgcggtcg tgcgtgtttt
    10621 cgttcagcgc taaaacgttg cgccggagca tgctgcggta aagagagcca tgaggaacat
    10681 gcgctacgct tgcgccagtc tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggg
    10741 gcggtggcgc tgaaagaaca gcacccggaa atgactcaat atcatattat tcaaaactgg
    10801 ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc
    10861 gggtttgatc acgacggtta taaaattctt tgtaagccgc tgctttccgg taactatgaa
    10921 attactgaac ttgatccggc gaatgaccag cgagccagtt gatttcactg aacaacagtt
    10981 taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc gaaccagcac gcgccctggt
    11041 cggtagtgag cagtttgtcg cccagttgcc acgccccgtt aatcacaaac accaccccgc
    11101 cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct gactttcgcc ttacagacat
    11161 ccaggcgcgt cataatattg aaatccatcg acatctgccc tgccgtcagt ttcgctttta
    11221 ccacctggtc cgccgcaaag gcaaaaggct gtaacggttt taaggtatgg ttaaagtggt
    11281 ctgcgctttc aaggaacatc tcgccgcctt ccagcaacgt cactatcctt tccataccag
    11341 gaaacaaaga aaactcccca ttcgccgcga tggatgcaat gctggcacgc cagtaaaaat
    11401 cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc accggcagcg tttcgccaca
    11461 aattcaccga cattttacgc atatcaaagt attccatact cgctcccggc ccgttctttt
    11521 cattgttatc gttcaggtaa ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt
    11581 ggtgatcagg ctttttttac aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc
    11641 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaagat
    11701 tattcagcag ttgcaggcat tttacctttt gccgctggac gttctgtcag acgcttctca
    11761 aaattagcat taaattgctt tttctgttcc ggcgtcagga tgttgtaaat tttgttctgg
    11821 gtttccatgt gcgccagcat gttagctttg cgctgttctt ccatttttgc gatctgcgct
    11881 tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt catgcattgc gcgacgttct
    11941 tccagcggcg gacgtttcat ctggtcacgc tggcctttca tgatttcgcg aatctgctgt
    12001 ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt cctgatgcgg accgaacttg
    12061 cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg cggtagtggt gtctgcggca
    12121 tgggccaggt tagccgcgcc aagagccagg gtagaggcaa caaacagtgc agttaattta
    12181 cgcatattct atatccttcc tttcagttat ttattacggc tttcttaagt agcgtgccgt
    12241 gttgacgaga ttaactttac tgactttagc gtcaattaat cagagcaacg gtaaaacaat
    12301 gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt tgatggagag
    12361 tgatgagaga atattacaac accatgattt tgcagagatt atgaagaact ataccggatg
    12421 actggtgata aa
//
LOCUS       DAZKIF010000126        12180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZKIF010000126 DAZKIF010000000
VERSION     DAZKIF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.127"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            229..999
                     /gene="yjjP"
                     /locus_tag="ACHOTW_003772"
     CDS             229..999
                     /gene="yjjP"
                     /locus_tag="ACHOTW_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP9845796.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            990..1463
                     /gene="yjjB"
                     /locus_tag="ACHOTW_003773"
     CDS             990..1463
                     /gene="yjjB"
                     /locus_tag="ACHOTW_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP9845797.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1570..2109
                     /gene="dnaT"
                     /locus_tag="ACHOTW_003774"
     CDS             1570..2109
                     /gene="dnaT"
                     /locus_tag="ACHOTW_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP9845798.1"
                     /translation="MSSRVLTPDVVGIDTLVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2112..2849
                     /gene="dnaC"
                     /locus_tag="ACHOTW_003775"
     CDS             2112..2849
                     /gene="dnaC"
                     /locus_tag="ACHOTW_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP9845799.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2898..3392
                     /gene="yjjA"
                     /locus_tag="ACHOTW_003776"
     CDS             2898..3392
                     /gene="yjjA"
                     /locus_tag="ACHOTW_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP9845800.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3646..5937
                     /gene="opgB"
                     /locus_tag="ACHOTW_003777"
     CDS             3646..5937
                     /gene="opgB"
                     /locus_tag="ACHOTW_003777"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP9845801.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6074..7096)
                     /gene="lgoD"
                     /locus_tag="ACHOTW_003778"
     CDS             complement(6074..7096)
                     /gene="lgoD"
                     /locus_tag="ACHOTW_003778"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFP9845802.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7235..8149
                     /gene="lgoR"
                     /locus_tag="ACHOTW_003779"
     CDS             7235..8149
                     /gene="lgoR"
                     /locus_tag="ACHOTW_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP9845803.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8364..9725
                     /gene="lgoT"
                     /locus_tag="ACHOTW_003780"
     CDS             8364..9725
                     /gene="lgoT"
                     /locus_tag="ACHOTW_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9845804.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9774..11438)
                     /gene="tsr"
                     /locus_tag="ACHOTW_003781"
     CDS             complement(9774..11438)
                     /gene="tsr"
                     /locus_tag="ACHOTW_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP9845805.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11667..>12180
                     /locus_tag="ACHOTW_003782"
     CDS             11667..>12180
                     /locus_tag="ACHOTW_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845806.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLLKEIN"
BASE COUNT         3018 a         3170 c         2921 g         3071 t
ORIGIN      
        1 ctgacccgta aaggctaaaa caataaattc attatagtga taatattcat gtgatagttt
       61 gtttgcggcg agagataatt cgctttttat caccgagctt gtcccgataa ccaggattaa
      121 tcagcgcaat tttgatctta acctaccgca aaacaacctt gcttcatgat acaaatggat
      181 tctcattatc tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag
      241 caacagcgag ccgtaacacg gctttgtatc cagtgtggat tatttctttt gcaacatggt
      301 gcggaaagcg cgttggttga tgagctttcc tcacgactgg gtcgggcgct gggaatggac
      361 agcgtcgaaa gttctatctc ttcgaacgcc atagtgctga caactattaa agatgggcaa
      421 tgcctgacat cgacacgtaa aaatcacgat cgcggcatta atatgcatgt ggtgactgaa
      481 gtccagcaca ttgtgattct tgcggagcat catctgctgg attacaaagg cgtagagaaa
      541 cgatttagcc aaattcagcc attacgttac ccaagatggc tggtagcctt aatggttggc
      601 ctttcttgcg cctgtttctg taaactcaat aacggtggct gggatggtgc cgtcatcacc
      661 ttctttgcca gtacgaccgc gatgtatatc cgccagctgc tggcacaacg tcatcttcat
      721 ccacagatca acttttgcct taccgctttc gccgccacca ccatttccgg attgcttttg
      781 caacacccca ctttcagcaa tacccccacc attgcgatgg ccgccagcgt tctgctgcta
      841 gtgccgggct ttccgttgat taatgccgtc gccgatatgt ttaaaggcca catcaatacc
      901 ggactggcac gctgggcgat cgccagtctg ctgacactgg ctacctgcgt cggcgtagtg
      961 atggcactga cgatttgggg gctacgcgga tgggtgtgat cgaatttctg ttagcgttgg
     1021 cgcaggatat gatcctcgcc gccattcctg cggtcggctt tgcgatggtg ttcaacgttc
     1081 ccgtgcgggc gttacgctgg tgtgcgctgc ttggctcgat aggtcatggt tcccgaatga
     1141 tcttgatgac cagcgggttg aatattgagt ggtcaacctt tatggcctcc atgctggtcg
     1201 gcaccattgg tattcaatgg tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg
     1261 cggccgttat ccctatgttc ccgggcatat cggcttatac cgcaatgatt tcggcggtaa
     1321 aaatcagcca gttaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta
     1381 cagcttcatc gattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt
     1441 accgcaagcg ccctcgcgta taaaattgcc tcgcttaagc acacggatga gagacagcct
     1501 cctctcctcc gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg
     1561 agaaacagta tgtcttccag agttttgacc ccggacgtcg ttggtattga caccctggta
     1621 cacgatcacc aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac
     1681 aatgccccgg cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa
     1741 gaaaagctgg cgcgtccggg aagcgatgtc gatctggacg atcaactcta tcaggaaccg
     1801 caagccgctc ccgttgctgt acccatgggg aaattcgcca tgtatccgga ctggcaaccc
     1861 gatgccgact ttatccgcct ggcggctcta tggggcgtgg cgctaagaga gccggtaacc
     1921 accgaagaac tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat
     1981 gtgcagtggc aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga
     2041 ctgccgaaac gagatgtgaa tacggtcagt gaacctgaca gccaaattcc accaggattc
     2101 agagggtaac gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg
     2161 cccatatcaa acctgcattc aaaacgggtg aagagctact ggcctggcag aaagaacaag
     2221 gggcgatccg ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct
     2281 ttaaccgctc cggtattcgc ccactgcatc agaactgctc ctttgagaac tatcgcgttg
     2341 agtgtgaagg gcagatgaat gcgttaagca aagcgcgcca gtatgtcgaa gagtttgacg
     2401 gcaacatcgc cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg
     2461 cggcaatctg caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg
     2521 atattatgtc ggcgatgaaa gataccttca ggaatagcgg taccagcgaa gaacaactgc
     2581 ttaacgatct gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat
     2641 cgaaatacga aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc
     2701 caaccgggat gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg
     2761 tgatggaccg tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc
     2821 gtagccgtgt aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa
     2881 ttccaccttg agtaatgatg atgaaaactg ttaaacatct tctgtgctgt gccattgccg
     2941 ccagcgcatt aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg
     3001 ccgccagcga acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt
     3061 cattaactaa cttgcttagc agcggaaacc aggccttaag cgcagataac atgaacaatg
     3121 ccgcaggcat tctgcaatac tgcgcgaagc aaaagctggc ttcggtaacc gatgtcgaaa
     3181 acatcaagaa ccaggtgctg gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca
     3241 ccaactatct ggacggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc
     3301 tggataacat cggaacaact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg
     3361 tgttaaagca ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta
     3421 cccggctgac gcggctctct cctgcccttt tccctgctcc tggacggttt tccccctgtt
     3481 acacgggaag cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc
     3541 atttttttga cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa
     3601 agcttaaacg ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc
     3661 tctttcgccc tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc
     3721 tggtggtttg cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcaccctg
     3781 tttgccagcg actattttac tggcgatggt attaacgacg cggttctcta taccttaacc
     3841 aacagcctga ccggtgctgg cgtcagcaaa tacattttgc cgggtatcgg cattgtgctg
     3901 gggctgacag cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg ccatcatccg
     3961 caccattttg gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc
     4021 ccggcatttc gtcagataac ggaactggtg aaatcccagt cacgcgacgg cgacccggac
     4081 tttgcggctt attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt
     4141 tatatctacg gcgaaagtct cgagcggacc tattttgata acgaggcttt cccggacctc
     4201 acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg
     4261 ccgggaacag attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt
     4321 gccccctttg aaggcaacgc ctccgcttct gtttccagct tcttcccgca gaacatctgt
     4381 ctgggcgata tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg
     4441 cgttttgccg gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca
     4501 gaagagctga aaagcgtggt agccgacccg cactatcgca acgactgggg attctacgac
     4561 gataccgttc tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga
     4621 ttctcactgt ttaccctgac agtcgatacc catcacccgg atggttttat ctctcgtacc
     4681 tgtaaccgca aaaaatatga ttttgacggt aaaccgaatc agtcattcag cgcggtaagt
     4741 tgcagccagg agaatatcgc gacgtttatc aacaaaatca aagcgtcacc gtggtttaaa
     4801 gatactgtca tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac
     4861 ctcaataagc aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa
     4921 gagacgctgg cagtgaagcg taacacgatg gataacggag cgacagtgct ggacattctc
     4981 ggtggcgata actatctcgg acttggtcgt agcagtttat ccgggcagtc aatgtcggaa
     5041 atcttcctca atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgtctgtgg
     5101 aaattcccta aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc
     5161 tcgggtagcc atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg
     5221 ctgccagaaa gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc
     5281 gacaatttcg tctgggttga ccgttgctac aagatggcac aactctgggc tccggaactg
     5341 gcactttcca ccgactggtg tgtctcgcag gggcaacttg gcggtcagca aattgttcag
     5401 catgttgaca aaacaacatg gaagagcaag acggcattta aagatacggt catcgacatg
     5461 gcgcgttaca aaggcaatgt cgatacgctg aagattgttg ataacgatat tcgctacaaa
     5521 gccgacagtt tcatctttaa cgtcgccggt gcgccagaag aggtgaaaca gtttagcggg
     5581 atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa
     5641 atcgagtaca agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac
     5701 ggcaataacg ccagccgtcc tattccggta cgcgtaggca atgaagaaca aactcttgtg
     5761 ctgggcaatg aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca
     5821 ctggtaattg tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg
     5881 ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg tagaacgtga agggtaattc
     5941 ctgactgatg tgtaggtcgc atagggtgcg acctacacac cttctccggc ttacaacaca
     6001 ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg gacgctgcat ggcaacttta
     6061 ataatctcgc ctttcagaaa gtaattacgc ctttaattaa ctcacgattg ttaatcacat
     6121 cgcgctcgta ggtttctgcc agcgtggcga acggatagcg atgggttaac atcatgccag
     6181 cagtgatttt tccttccgcc atcagtcgac cgactttagc aaaatcttcc ggcgtggcgt
     6241 tgcggctgcc catcatcgtc gtttcttttt tatggaattc cggatcggag aactgcaact
     6301 cacctttaaa caggccgaca aataccaccg tgccgccgtg acgaatcaga ttcacggtgt
     6361 tattcatcgc atgttgatta cctgtcgcgt cgatcacttt ctgcgccagc gaaccaccaa
     6421 actgcgcccg cagctgcgcg tcaaaatctt cggctgacgg gtccagcacg ggcaactcca
     6481 gacgcgttgc cacatgttca cggcgcgccg gactggtatc cgccaccacc acctgtgcgc
     6541 catcggcttt agcgattgcc gccgcgccca gaccgattgg cccggccccg accaccagca
     6601 cctgctcgcc gggagcaatg gctgcgcgac gcaccgcatg agcgctaata gcgaaaggtt
     6661 caatcaatgc ggccgcctgc gggtcaatac cgtctgcggg caaaatgttc gccaccggca
     6721 cgctcaaata ctcactaaaa ccgccatcct gatgcacgcc aatgactgaa attttttcac
     6781 agcaattggt acgcccgctt ttacacgccg ggcattgctg acaggcaaca taagggatca
     6841 cagcaacttg ctgaccattt ttaagatcag caatattttt acccagccca acaatctccc
     6901 cacatatttc atggcctaaa acccgtggat aactaaaaaa tggttgattt ccaccccagg
     6961 catgaatatc ggtaccgcaa atcccgacag acttaatttt tattaatgct tcattgtcac
     7021 ccggaatagg tatctcgcgt tgtttccaga ctaattcttt cggctgctgg caaattaaaa
     7081 cattcatcgt agacatgatt cggctctcca ttttttgttg ccttagatat caatgaaaat
     7141 gaaaaatggc agcggaacgg aaaatctgtt ttgtgaaaac acacgcataa aaatgtttta
     7201 aatcgggttt taatggactc acaaggagga aaatatgagt cgttcacaaa atttacgcca
     7261 caatgtgatt aaccaggtca ttgatgatat ggcgcggggt catatccctt ccccacttcc
     7321 atcgcaaagt gcgctggcgg aaatgtacaa catcagccgc actacagtgc gtcatattct
     7381 cagccactta cgcgaatgcg gcgtcttaac gcaggttgga aacgactatg ttattgcgcg
     7441 taaacctgac catgatgacg gtttcgcctg tactaccgct tcaatgagtg aacaaaacaa
     7501 agtgtttgag caggcttttt tcacgatgat aaaccagcgc cagttacgcc caggggaaac
     7561 tttttctgaa ctgcaactgg ctcgggccgc gggtgtcagc ccggtggttg taagagaata
     7621 tcttttaaaa ttcggccgtt ataatcttat tcaaagcgaa aagcgcggcc agtggagcat
     7681 gaagcaattc gatcaatcct acgctgagca gctgtttgag ctacgcgaaa tgctcgaaac
     7741 acattcactt cagcactttc tcaacctgcc tgatcatgat ccccgctggc tgcaggcgaa
     7801 aaccatgctc gaacgtcatc ggctactgcg tgacaacatc ggcaatagct ttcgcatgtt
     7861 ctcgcaactt gacagagatt ttcactcctt actgctttca gctgccgaca atatcttttt
     7921 tgatcaatcg cttgagatca tctcagtcat ctttcatttt cactatcaat gggacgaaag
     7981 cgatcttaaa cagcgcaata tcattgccgt cgatgagcat atgaccattc tcagcgctct
     8041 gatttgccgc agtgaccttg atgccaccct tgcgctccgt aaccatttga attcagcgaa
     8101 acaatcaatg attcgatcca ttaatgagaa cacgcgttat gctcattaag tacggattaa
     8161 aaacagcaaa ctattagcgg gtgcacggtt ttatcaaaag ataaataccg ggaaattccc
     8221 gcttacctat gctcacaatc agtcgactaa aaatgttttt gtgagcggta gtaaagtcct
     8281 aaaactttaa cctgccgtta atgtcaacct tgcgtaatgc acaattacgt aaacaattaa
     8341 aattataaat acaggagtcc ggcgtggaaa aagaaaatat caccctcgat ccgcgttctt
     8401 catttactcc atcttcgtcg gcagatattc ccgtgccacc agatggatta gttcaacgca
     8461 gtacccgaat taaacgcatt caaaccaccg ccatgttgtt attatttttt gcggcggtaa
     8521 tcaattatct cgaccgcagt tcgctgtcgg tagcaaattt aacgattcgt gaagaattgg
     8581 gattaagtgc caccgaaatc ggcgctttgc tctccgtgtt ttcactcgct tacggcattg
     8641 cgcaacttcc ttgcggccca ctattggatc gtaaaggccc acgcctgatg ctgggactgg
     8701 ggatgttctt ctggtcactg ttccaggcca tgtctggcat ggtgcacagc tttacgcagt
     8761 tcgtgttggt gcgtatcggt atggggattg gtgaagcgcc gatgaaccca tgcggtgtaa
     8821 aagtcattaa cgactggttc aacatcaaag agcgcggacg cccgatgggc ttcttcaacg
     8881 cagcttctac cattggcgtt gccgtaagcc caccgattct ggcggcgatg atgctggtga
     8941 tgggctggcg cgggatgttt attaccattg gtgtactggg gatttttctc gccatcggct
     9001 ggtatatgct ctatcgcaac cgcgagcacg tagaactgac tgccgttgaa caagcttatc
     9061 tcaatgcagg tagcgtcaat gcccgccgag atccgctcag ttttgccgaa tggcgcagcc
     9121 tgttccgtaa ccgcacaatg tggggaatga tgctcggatt cagtggcatc aactacactg
     9181 cgtggctgta tctggcctgg cttcctggtt acctgcaaac agcctataac ctggatttaa
     9241 aaagcacagg gttgatggcg gctatccctt tcctgtttgg tgctgccggg atgctggtca
     9301 acggttacgt tactgactgg ctggtcaaag ggggaatggc tccgattaaa agccgtaaga
     9361 tctgcattat tgccgggatg ttctgttctg ccgcctttac gctgatagta ccacaagcga
     9421 caacatccat gatggcggtt ctgctgattg gcatggcact gttctgtatt cactttgccg
     9481 gaacatcctg ctggggcttg atccacgtcg cagttgcttc tcgcatgact gcgtcggtgg
     9541 gcagtatcca gaactttgcc agcttcatct gcgcctcttt cgcgccgatc attactggtt
     9601 ttattgttga tacaacccac tcattccgtc tggcactaat catttgcggt tgcgtcaccg
     9661 cagcgggagc actggcgtac atcttcctgg ttcgtcagcc gatcaacgac ccgcgtaaag
     9721 attaaactcc ctatgcccga taacattttg cttatcgggc atttttatgg cgattaaaac
     9781 gtttcccagt tctccccgct atctgccact gccattttac gcggcgtagc tggcgtcacg
     9841 gtttttacca cagccgatgt ttcacgctgc tgttgctgct gttgctgaat ccggaacact
     9901 gccacagctt cggtcaggcg actggcctgc tcttccaacg cggcggcggc agcggcagac
     9961 tcttccacca gcgcagcgtt ctgttgagtt acccggtcca tctcagcaac cgctaagcca
    10021 acctgatcga taccacggct ctgctcatca gaagcagaag ctatttcgcc cataatgtcc
    10081 gtcacgcggg tcacagcact gacaatctcc gccattgttt ccccggcgct ttcgaccagc
    10141 gtagagccaa catccacttt ccccaccgag tcttcaatca ggcttttaat ttcacgagcc
    10201 gcctgggcgc tacgctgggc cagattacgc acttctcccg caaccaccgc aaaaccacgt
    10261 ccttgctcac ccgcacgcgc tgcttcaacc gccgcgttca aagccagaat attggtctgg
    10321 aaggcaatgc cgtcaattac gctgataata tcggcgattt tctgcgaact ggtggagata
    10381 tcacgcatag tctgcaccac gttatctacc actttgccgc cgcgttgcgc cgtttcagaa
    10441 gcacttaacg ccaggtggct ggcctggcgc gcattctcgg cgttctgttt caccgttgcg
    10501 gtgagttgct ccatgctggc tgccgtctct tccagcgaag cggcctgttg ctcggtgcgc
    10561 gaagagagat cgttattgcc ggtagcgatt tcgctggcac cgctatagat ggcattggcc
    10621 ccgttgcgca catcaccgac ggtacgcatc agctctccct gcatatggcg caaactctct
    10681 gccagttgcc ccatctcatt agagccatcc acctcaatcg gtttcaccag atcgccgcct
    10741 gcaatatgac gaatgctgtc aatcaggcga ttcattggcg ctaccagcga ggctttaata
    10801 ccgaaccaga cggcgaagat gaccgccagt acgacgatca tcacgcccac cagaatccac
    10861 atcgcctggc tgtaggaggc attgttatcg ctgacggcga tatcatagag ccgatcgttt
    10921 tgctccatgt aagccacata ctgcttctcg aaaccgtcct gatatccctg ggtcggctga
    10981 tcaaagaact cgttgatttt gcctgcacct aacagttgga tcagctccgc cagcgcattg
    11041 tgataaatat cgtaattacg tttgatctct gccgctgcgg cggtgctctg acgcgggtca
    11101 cgcggcaacg cttcgtaatc tgcccagttt ttttccgcct gtttcaacga aatactggca
    11161 ctctgcatca gctcagcaac ggttgaaccg ctaccaatat tattctgatc catcatgtag
    11221 cggatacccg cgcggttgag ggtgttacgc gtctgcaaca acgcgaccca gctgccattc
    11281 agcgtggatt gctgctggcg aatggtttgt aaaacagtga aattttcttt gtcattcttt
    11341 aaggcattaa agaacagacc gcctgatgtc agttgtaaaa ggccaaaaac ggccaaaacc
    11401 agcagtaagc tggtcacgat cttgatacgt tttaacatgg tttctctttc ctgtggaccg
    11461 atgcaaaatt ttcggcctga aatgagaaaa ctttataggt agcgacttga aactggttaa
    11521 ttttacctca ctttttttaa tttttgttca aaaatacaat aaccacttat tgatacattt
    11581 tcttttctta tttttttcac acgaattgta tctattttga acgaaatatt ttatagagtg
    11641 tggaatttta agaaaggaca tctgaaatgt taaaccgtga cttcgatcat ctctcaacgc
    11701 tattacatct cttttttgaa cgtgaggata tttgccaaaa gcttaatatt attgaccaaa
    11761 acaatatcac cggttgggaa gtgtggttcc aggtagagtt tgctaatatg ctctgctcaa
    11821 cagaccatga atggtggcgt gaacaagcac tttcctgtga tatgcgcaaa aaacctgagc
    11881 gcccgacact acgcacagat ttcttactac gtaaaaaagg ctgggcacaa gatagctata
    11941 tcgcgcttga aataaaacaa aacagagacg cgaccagttg tgttaaaaat atgatcgcag
    12001 acttagaaaa aagcgcaaaa ataaaacgtt ccgaactcga tttacgcagt ttctggacct
    12061 tgggtatcac ccctatgata gacagggaaa ggctggactc tctcatcgat tgctatttag
    12121 atgagaaatt ttatcttact aaatctcgta aaaaacacgt tttacttaaa gaaataaata
//
LOCUS       DAZKIF010000127        12090 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZKIF010000127 DAZKIF010000000
VERSION     DAZKIF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.128"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            79..681
                     /gene="yebB"
                     /locus_tag="ACHOTW_003783"
     CDS             79..681
                     /gene="yebB"
                     /locus_tag="ACHOTW_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP9845807.1"
                     /translation="MNINYPAEYEIGDKVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(683..1204)
                     /gene="ruvC"
                     /locus_tag="ACHOTW_003784"
     CDS             complement(683..1204)
                     /gene="ruvC"
                     /locus_tag="ACHOTW_003784"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP9845808.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1239..1979)
                     /gene="yebC"
                     /locus_tag="ACHOTW_003785"
     CDS             complement(1239..1979)
                     /gene="yebC"
                     /locus_tag="ACHOTW_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9845809.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(2008..2460)
                     /gene="nudB"
                     /locus_tag="ACHOTW_003786"
     CDS             complement(2008..2460)
                     /gene="nudB"
                     /locus_tag="ACHOTW_003786"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP9845810.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2578..4350)
                     /gene="aspS"
                     /locus_tag="ACHOTW_003787"
     CDS             complement(2578..4350)
                     /gene="aspS"
                     /locus_tag="ACHOTW_003787"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9845811.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALCVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            4660..5226
                     /gene="yecD"
                     /locus_tag="ACHOTW_003788"
     CDS             4660..5226
                     /gene="yecD"
                     /locus_tag="ACHOTW_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9845812.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5223..6041
                     /gene="yecE"
                     /locus_tag="ACHOTW_003789"
     CDS             5223..6041
                     /gene="yecE"
                     /locus_tag="ACHOTW_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9845813.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6094..6489
                     /gene="yecN"
                     /locus_tag="ACHOTW_003790"
     CDS             6094..6489
                     /gene="yecN"
                     /locus_tag="ACHOTW_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP9845814.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6530..7273
                     /gene="cmoA"
                     /locus_tag="ACHOTW_003791"
     CDS             6530..7273
                     /gene="cmoA"
                     /locus_tag="ACHOTW_003791"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP9845815.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7270..8241
                     /gene="cmoB"
                     /locus_tag="ACHOTW_003792"
     CDS             7270..8241
                     /gene="cmoB"
                     /locus_tag="ACHOTW_003792"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP9845816.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8406..10835)
                     /gene="torZ"
                     /locus_tag="ACHOTW_003793"
     CDS             complement(8406..10835)
                     /gene="torZ"
                     /locus_tag="ACHOTW_003793"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP9845817.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10860..11960)
                     /gene="torY"
                     /locus_tag="ACHOTW_003794"
     CDS             complement(10860..11960)
                     /gene="torY"
                     /locus_tag="ACHOTW_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP9845818.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLMAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2772 a         3199 c         2940 g         3179 t
ORIGIN      
        1 atatacattg cgcctcctga aaaaataaat tttttatgct attacgtata ttcgtatcta
       61 tttcaatgga atgacaacgt gaatattaat taccctgctg aatatgaaat tggagataaa
      121 gtctttacat gtataggtgc tgccttattt ggtcaaatat cagctgcatc aaattgctgg
      181 agtaatcacg tcgggatcat tatcggtcat aacggtgaag acttcctggt tgcagaaagc
      241 cgggttcccc tttcaaccat caccacgcta tctcgtttta ttaaacgttc gtctaatcaa
      301 cgctatgcta taaagcgatt agacgctggg ctgacagaac aacaaaaaca acgaattgtt
      361 gaacaggttc cctcccggct acgcaaactt taccacaccg gttttaaata cgaatcttcg
      421 cgccagttct gttcaaaatt tgtttttgat atttataaag aagcgctatg tattccggtg
      481 ggtgaaatag agacgtttgg agaattgtta aatagcaatc cgaatgcaaa actcactttc
      541 tggaaattct ggttcctggg ttctattccg tgggagcgta aaaccgtcac gccagccagt
      601 ttgtggcatc atccaggttt ggtgttgatt catgcggagg gagtggaaac gcctcagccg
      661 gaactgaccg aggcggtata acttaacgca gtcgccctct cgccaggttc agccgcgatt
      721 cgctcatctg catcgcattc tgactaacgt ggcagtgggt gatggcaatc gccagcgcat
      781 cggcggcatc cgcctgtgga ttagcgggca gtttcagcaa ggtgcggacc atatgctgca
      841 cctggctttt ttcggcacta ccaataccta ccaccgtttg ctttacctga cgtgccgcgt
      901 attcaaatac tggcaactcc tgattcaccg ccgccacaat cgccacgccg cgcgcctggc
      961 ccagtttcag ggctgagtca gcgttctttg ccataaagac ttgttcaatg gcgaaataat
     1021 cgggctggaa ctgggtgatg atttccgtca cgcccgcata gatgagtttc agacgagacg
     1081 gtaaatcatc cactttggtg cgtatgcatc cgctacccag gtaggacagt tgcctaccta
     1141 cctggcggat gacgccgtag ccggtcacgc gcgaacccgg atcaatgccg agaataatag
     1201 ccatcacgcg tctccgtttt gctgtttagc aggcctcatc agagagtcgc tgcgacctca
     1261 tcagagattt caccgttatg gtaaacttcc tgcacgtcgt cgcaatcttc cagcatatcg
     1321 atcagacgca tcagtttcgg tgcggtttct gcatccatat cagctttggt agacgggatc
     1381 atggaaactt ccgcgctgtc tgctttcaga cctgccgctt ccagagcgtc gcgcacttta
     1441 cccatttctt cccatgcagt gtagacatca atcgcgccgt catcataggt cacaacgtct
     1501 tcagcacctg cttccagtgc tgcttccatg atggtgtctt catcgccttt ctcgaaggag
     1561 atgacgcctt ttttgctgaa cagataggct acggaaccat cagtaccgag gttaccgcca
     1621 catttgctga atgcatgacg cacttcagca acggtacggt tgcggttgtc agacagacat
     1681 tcaatcatga ttgccgtgcc gccaggaccg taaccttcgt agatgatggt ttccatgttc
     1741 gcatcatcat caccgcccac accacgtgca attgcgcggt tcagagtgtc acgggtcatg
     1801 ttgttagaca gtgctttatc aattgctgca cgcagacgcg ggttagcgtc cggatcgcca
     1861 ccgcccagct tagccgcggt taccagctca cgaatgattt tagtgaagat tttaccgcgc
     1921 ttagcatcct gcgcagcttt acgatgtctg gtgttggccc atttactatg acctgccata
     1981 aaaatatctc cagatagccc tgcctgttca ggcagcgtta attacaaact gttcaatcgc
     2041 ctgccggttg ctccaggact tagtgagcgc cgccgcagca ggcgcatcaa gccacttgta
     2101 agccagatgt tcagtgaaaa cgatctgccg ctcatgcgga agcgcaagac agaaccatga
     2161 ttccgtatta cgcgtcacgc ccggcgcata gcgatgacgt aaatgtgaaa aaatttcaaa
     2221 ctctaccgtg cgctgacagt caattaaggt cagttgttca gcgacaacat caatggtgac
     2281 ctcttccttt acttcgcgca tggcagcttg cggcgcggtt tcaccctctt ccacgctgcc
     2341 ggttaccgac tgccagaaat cggggtcatc acgccgctgc aacatcagca cccgtttcgt
     2401 atcttgtgcg tagatgacca ctaagatcga aacgggacgc ttatacactt tatccttcac
     2461 gctgcctctt cgttgactgc cttcgctcat cccattcaca tagttatcta tgctcatggg
     2521 agttcactca gttgccgcct cgatgcaacg cgaatgattt cgtgtatttg agtcatatca
     2581 gttattctca gccttcttca caacctgaat gctcagctca gccagtgcag ccgggttagc
     2641 aaagctcggt gcttcagtca tcagacacgc tgccgccgtg gttttcggga aggcgataac
     2701 gtcacggata ttgtcggtgc cggtcagcag catggtcaga cggtcaagac cgaatgccag
     2761 acctgcgtgc ggcggagtac cgtatttcag agcgtcgagc aggaagccga atttctcgcg
     2821 ctgttcctct tcgttgatac ccagaatacc aaacaccgtc tgctgcatat caccattatg
     2881 gatacgtact gaaccaccgc ccacttcgta accattgatg accatatcgt aagcgttcgc
     2941 caccgcattt tccggtgcag ctttcagctc tgcagccgtc atgtctttcg gtgaggtgaa
     3001 cggatggtgc attgccgtca ggccgccttc accgtcgtct tcaaacatcg ggaagtcgat
     3061 aacccacagc ggtgcccatt tgctttcgtc ggtcagacca aggtctttac ccactttcag
     3121 gcgcagtgcg cccatcgcgt cggcaacaat tttcttgttg tcggcaccga agaaaatcat
     3181 atcgccatct tgcgcggcag tacgatccag gatggcttcg atgatttctg cattaaggaa
     3241 cttcgctacc gggctgttga taccttccag acctttcgcg cgttcgttaa ctttgatgta
     3301 agccagacct ttcgcgccgt agattttaac gaagttaccg tattcgtcga tctgcttacg
     3361 ggtcagcgat gcgccacccg gaacacacag agcggcaacg cgacctttcg gatcgttcgc
     3421 cggacctgca aatactgcaa actcaacaga tctcagcaga tcagcaacgt cggtcagttc
     3481 catcgggtta cgcagatccg gtttatcaga accataacgg cgttcggctt ccgcaaaggt
     3541 cattactggg aaatcgccca gatccacgcc cttcacttcc agccacagat ggcgcaccag
     3601 cgcttccatc acttcacgca cttgcggcgc ggtcatgaaa gaagtttcca catcgatctg
     3661 agtaaattca ggctgacgat cagcgcgcag gtcttcgtca cggaagcatt taacgatctg
     3721 atagtagcgg tcaaagccgg acatcatcag cagctgtttg aacaactgtg gggattgcgg
     3781 cagtgcgtag aatttacctt tgtgcacacg agaaggcacc aggtagtcac gcgcgccttc
     3841 cggcgtggct ttggtcagca tcggagtttc gatgtcgagg aaaccgtggt catccataaa
     3901 acggcgcacc aggctggtga ttttagcgcg ggttttcagg cgctgagcca tttccggacg
     3961 acgcaggtcg aggtagcggt atttcagacg cgcttcttcg gtgttgacgt ggttagagtc
     4021 aagcggcaga acatctgcgc ggttgatgat agtcagcgag gacgccagca cttcgatttc
     4081 gccggtcgcc atatcgcggt taatattttt ttcgtcacgc gcacgtacgg tgcccgtgac
     4141 ctgaatgcag aactcattac gcagttcaga ggccagcttt aacgcgtccg cacgatccgg
     4201 atcgaaaaat acctgcacga taccttcgcg gtcgcgcata tcgatgaaga tcaggctacc
     4261 aagatcacga cgacggttga cccaaccaca cagagtcacc tgctgcccca cgtgggacaa
     4321 acggagctgt ccacaatatt ctgtacgcat gagatatccc ttaacttagc tgccggcgga
     4381 tgccccctgc tgcgcaggtg accaagtcgc agcgttagct gtatgtcaca actgaatgaa
     4441 aaaaggcggc tattatactg gaaattctgc cgcaccgtaa gagcctggcc cgcgctggaa
     4501 cgcctcgtta ccactttata tcgggcctga aatcagactc tacgccagtt tgctataaag
     4561 gtgttgcccg aactcataca aattaacaaa atttgtcgtt ccgccatcgg ctaatcgcat
     4621 taaggtgtga ggcacgattt tgttttttca ggagtcatca tgcttgaact taatgctaaa
     4681 accactgcgc tggtggtgat cgatttacaa gaaggcatct taccttttgc cggtggtcca
     4741 catactgccg atgaggtagt taatcgcgcc gggaagctgg cggcgaaatt tcgcgccagc
     4801 ggtcagcccg tgtttctggt gcgcgttggc tggtctgccg attacgccga agcattaaaa
     4861 cagccggttg atgccccttc ccccgctaaa gtgttgcccg aaaactggtg gcaacatcca
     4921 gctgcattag gtgcaactga cagtgatatc gaaataatca aacgtcagtg gggtgcgttt
     4981 tacggtacgg atctggagtt gcaattacgc cgccgaggta tcgatacaat agtgttatgt
     5041 gggatctcga ccaatatcgg agttgaatcc accgcccgca atgcctggga acttggcttt
     5101 aatctggtga ttgccgaaga tgcctgtagt gccgctagcg ccgagcagca caataacagt
     5161 atcaaccata tctacccgcg catcgcccgt gtgcgtagcg tggaagagat cctcaacgcg
     5221 ttatgattta catcggtcta ccgcaatggt cgcatcctaa atgggtgcgg ttggggatca
     5281 ccagccttga agagtatgcc cgtcacttta actgcgtgga gggcaacacc acgctttacg
     5341 ccctgccgaa acccgaggtt gtcctgcgct ggcgtgaaca gaccacagat gacttccgct
     5401 tctgttttaa gtttccggcg accatttcgc atcaggcagc attacgacat tgcgatgatt
     5461 tagtgactga atttttgacc cgcatgtcac cgttggctcc gcgcattggg caatactggc
     5521 tgcaactgcc tgccacattc ggcccacggg agctgcctgc gctttggcat tttctcgatt
     5581 ctctccctgg cgaatttaat tatggtgtgg aagtccgcca tccacagttt ttcgccaaag
     5641 gggaagagga acaaacgctt aatcgcggtt tacatcagcg cggcgttaat caggtgattt
     5701 tagacagccg cccggttcat gcagcacgtc cacacagtga agctattcgc gacgctcaac
     5761 gaaaaaaacc taaagttccg gtacatgctg tactgacggc gacaaatccg ctgatccgtt
     5821 ttatcggtag tgatgatatg acgcaaaacc gggaattatt tcaggtctgg ttacaaaaat
     5881 tagcgcagtg gcatcagacc actacgcctt atcttttttt acatacgcca gatattgccc
     5941 aggccccgga actggtacat accctgtggg aagacttacg taaaacgctt ccagagatcg
     6001 gagcagttcc ggctattcca cagcaatctt ctcttttctg aatttgccac ctatcataga
     6061 caggtgccat cggccatttt aaagggagtt tgtatggtaa gcgcgctgta tgccgtttta
     6121 agtgcgttgt tattaatgaa gttctctttt gatgtcgttc gcctgcgaat gcagtaccgc
     6181 gttgcctatg gtgacggtgg ttttagcgaa ctgcaaagcg ctattcgcat tcatggtaac
     6241 gcggtggaat atattcccat cgcgattgtg ctgatgctgt ttatggaaat gaatggcgca
     6301 gaaacctgga tggtgcatat ttgcggcatc gttttgcttg ctggtcgtct gatgcattat
     6361 tacggttttc atcaccgtct gttccgctgg cgacgttccg gcatgagcgc cacctggtgt
     6421 gcgctgttgc tgatggtgct ggcgaatctt tggtatatgc cctgggagtt ggttttctcc
     6481 ctgcgttagc gcacaatacg ccactttctt tttcccggat ttttacgtta tgtctcaccg
     6541 cgacacgcta ttttctgccc ctatcgccag actgggcgac tggacctttg atgaacgggt
     6601 agctgaagtc ttcccggata tgatccagcg ttccgttccc ggctattcca atattatttc
     6661 catgattggt atgttagccg agcgcttcgt tcaacctggt acgcaggttt acgatctggg
     6721 ttgttctctg ggcgcggcga cgctctcggt gcgtcgcaac attcatcatg ataattgcaa
     6781 aattattgcc atcgacaact ccccggcgat gattgaacgc tgccgtcgtc atattgacgc
     6841 ctataaagcc cctacgccag tagacgtcat tgaaggtgat attcgcgata tcgccattga
     6901 aaacgcatcg atggtggtgc tgaattttac cctgcaattc ctggaacctt ccgagcgcca
     6961 ggcgttactg gataaaattt atcaagggct gaaccccggc ggcgcgctgg tgctttcgga
     7021 aaaattcagt ttcgaagatg ccaaagttgg tgaactgttg ttcaacatgc accacgactt
     7081 taaacgtgcc aacggttaca gcgaactgga gatcagccag aagcgcagca tgctggaaaa
     7141 cgtgatgctg accgattccg tggaaaccca taaagcacgc ctgcataaag ccggttttga
     7201 gcatagcgag ctgtggttcc agtgctttaa ctttggttca ctggtggcat taaaagcaga
     7261 ggacgctgca tgatcgactt tggtaacttt tattctctga ttgccaaaaa tcatctttca
     7321 cactggctcg aaacgctgcc cgcgcagatt gctaactggc agcgcgagca gcagcacggg
     7381 ttgtttaagc agtggtcaaa tgcggtggag tttctacctg aaattaaacc gtatcgtctt
     7441 gatttattgc atagcgttac ggcagaaagc gaagagccac tgagcgcagg gcaaattaaa
     7501 cgcattgaaa cgctgatgcg caacctgatg ccgtggcgca aagggccgtt ctcactgtat
     7561 ggcgtcaaca tcgataccga atggcgttcc gactggaaat gggatcgtgt tctgccccat
     7621 ctttctgatt taaccggacg caccattctg gatgtcggct gcggcagcgg ttatcacatg
     7681 tggcgcatga ttggcgcagg cgcgcacctc gcggtgggga tcgaccctac gcaactgttt
     7741 ctgtgccagt ttgaagcggt gcgtaaacta ctgggtaatg atcagcgcgc gcatttgtta
     7801 ccgttaggta ttgaacaact tccggcactg aaagcctttg ataccgtctt ttcgatgggc
     7861 gtgctttatc atcgtcgttc accgctggag catctctggc agttgaaaga tcaactggtc
     7921 aatgaaggtg aactggtgct tgaaacgctg gttattgatg gcgatgagaa cacggtgctg
     7981 gtgccgggcg atcgttacgc gcaaatgcgt aatgtctatt tcatcccttc cgcgctggcg
     8041 ctgaaaaact ggctgaagaa gtgtggtttt gtagatatcc gcattgcgga tgtgagcgtt
     8101 accactacag aagagcagcg acgcactgaa tggatggtta ccgagtctct gtccgatttc
     8161 ctcgacccac atgatccgag taagacagtt gaaggttatc ctgcgcctaa acgcgcggtg
     8221 ctgattgcgc gcaagccgta aaggtctggt attactgccg gatgcggcgt gaacgcctta
     8281 tccagcctac aaaaccttgc taattcaata tattgcaggg actatgtagg cctgataagc
     8341 atagcgcatc aggcagcttt atgtttgcat aacctcagcg cccgtttccg ggcgcttttc
     8401 acgtcttatg cctgaacagc tggctgatca aacgccgtta acttcggtgc gttacccgta
     8461 tatttttcga tatacaccag cgcagagtta ccggcacagg catttgccag ctgcgagctg
     8521 gggatatccg ccgttaacac gttcgcactg ccgtttttac acaagccatt ttccagatct
     8581 ggccatgcac cttcatgcac gcataccacg ccttttttga tcccgtcagt caccacagcg
     8641 cctgtcaaaa tctgaccacg tttgttccag acacggacca gatcgccatt cgcaatacca
     8701 aagcgagcag catcttcggt gtgaatagtg attggttcac gatcggcaac cgcatatttt
     8761 ttacgcagtt gcgcatagtt aagctgactg tgtaaacggt gtgccggatg tgcggtcaga
     8821 agctgtaact gcttctcgtc ggcggtacct ttccactcat caggcgcaag ccaggttggg
     8881 tgtgccgggc aatccttata gccaaatttt tccagcgttt tggagtaaat ctcaattttg
     8941 ccgcttggcg tacccagcgc atttttcacc ggatcggcgc ggaaatcacc ataacgaacg
     9001 tactgttcgt tcttctcgct gcggcgcatt tcgatcagtt tattttgctg ccagaaggca
     9061 ttaaacattg gcatagtgac gcgttgcgca cgggcacctt tctgagcggc atcatagaaa
     9121 aatttcagcc acgccatttc atctttacct tcggtataga tctcttttcc gcctggtttc
     9181 agtaattccg caagttcggc aaacacgtca aaatcgtttc gcgcttcaaa ttgcggagcg
     9241 acagcctgct tcatcggtac aatatgctgg ttgctgtaat caccggtcat cgtcaagtca
     9301 ttgcgctcaa acgatgtggt gatcggtaat acgatatctg catgtttagc agctgcggtc
     9361 cagtagcatt cagaaacgac gatcatctcc ggtttctgcc atgctttgat cagacgatta
     9421 gtatcctggt gatgggtaaa gttaccgcca cccgcccacc agatcatttt gatattcgga
     9481 tacgtttgtt ctttaccgtt atgctgatat ttgccgccag gattttccag cgcatcgacg
     9541 atacgggcaa caggaatcgc cgtcattccg ccatcatcag cggcctcgct ggcctgtccg
     9601 gcaatcgccg cagacatttc cggcagcacg ccgccaacac gcgtcgggtt gccaccgttg
     9661 gagtaatgat aagagaaacc aaaaccgccg cccggtgtac caatctgccc taacatcgcc
     9721 gccagcgtca ccagcatcca gtgtttctgt tcaccatatt gctggcgctg aattccccag
     9781 cctgccatca gcattgtgcg attcgccgcc atcagttcag cgagtttgac tatctgcgct
     9841 tccggtacgc cagtaatttc cgccgtccag gccgcgctct tcggagtatt atcgctctta
     9901 cctgtcagat actcttcaaa ctgcggatac ccggtggtgt atttttcaag gaacacttta
     9961 tcgtgtttgc cttgtgtcat cagggtatgc gcaatcccta acatcagtgc cacgtcggtg
    10021 cccatattcg gcgcgatcca ggtggcatta tagccaaaga actcgatggt ttcagagcgg
    10081 ataggatcaa tggcaatcac tggtttgcca gattttttca gctgatggaa gtattccagc
    10141 ccttgctcat cggtactgct ccaggcaatt tttaaggtat tcagcgggtt cattccccac
    10201 agcaccacaa cctggctgtt ttccagaatc agcggccagg aggtctgctg ttcatacacc
    10261 tctacagaac cgaccacatg cggcatgatc acctgtgctg ccccggttga ataatcgccg
    10321 ctatgcccgg aataaccgcc cgccaggttc atataacgtt gcagtaaggt ttgcgcttta
    10381 tgcaacacgc cagaagagcg ccagccgtaa gatccggcaa aaatggccga tggtccgtta
    10441 gctttacgaa tacgctcatg ttgctcatga atcagtttta atgcctgttc ccagctcacc
    10501 tgtacatagg tatcttcgcc acgacctttc gccggttgca gtggattgtc gagatagctt
    10561 tttctcacca tcggatgctg aatacgcgcc gtggtgtgta cctgatccgc cgccgtagac
    10621 tgtaaggagt tcggtatggt tttcgccagc gcgcctgtcg aagaaacaat cttgccgtcc
    10681 ttcacttcta cgttcatcgc tccccaacgt cccgcggtga ggattttacc gcccttctct
    10741 tctgcccatg cgggtagcgg tgctgccgat gtcaccacca gcgctccagc ggcaataccg
    10801 ctgtgtttaa taaattcacg tcttgttaat gtcataactt cctccctgat caacgaggat
    10861 cactgtttct cggtaatatc tttggcgttg tactggaaat accgcgttaa aatgtccagt
    10921 tcgttttcgc tcatgctggt tcgtgccccc attcctttgg caatggacgg ccacgcattg
    10981 acggtgtaat ggtcggcggc aataggggca tgacaaccag cgcaataggt atcgtcaagt
    11041 ttttcagcgt attgccatag cagtttacgg tccgctaatg cgggatcggt aagcgcaccc
    11101 tgtaaagacg cctgacgcca ttgattgccg tattcgtcag cctgccattc cccgtttacc
    11161 gtgagcgcct tgataccttc ttcacttaat gtggctagcg ccagccgttg acctgccgcc
    11221 aggtagagcg tgttttcact gccctgcatt tgataaccct gcaacagaac gatcggctgt
    11281 ttgccactgg catcaacgac ggtgagatcg gtaccaggat tcacggtagc cagctcgcct
    11341 atgcgagaag ttttgaaagg ataaatatgt gcgccattag tcactgaagt agcggcctga
    11401 ctttccagct catgcgccgc gttgtcatcc atttttattt ctggcggaaa atgggcaatg
    11461 cctttatgac aatcgataca ggtttcgccg cccttttgtg ctttgttatg cattttctgc
    11521 gcagattcac tttgcgaggc aatatccatg gcatcaaaag aatggcaact acggcacgtt
    11581 gcagagtcag ttgcttttaa ttctttccat actgtttcgg ccatttcctg gcgatgagct
    11641 tcgaacttat cgtcactgtc tattttgccg ctaacaaatt catgataaat atctttagat
    11701 gcttttaatt tagcgaataa ataatccatc cctgactttg gaatatggca atcggcacat
    11761 tccgcacgta tccctttctg gttcgaaaag tggacagttc cctgatattc ctcaaaaggt
    11821 ttactcatcg agtggcagga aaggcaaaat gctgtatccg acgttttatg taaggctttt
    11881 tgcgccatca agaacccgcc accaccaacc acaaccgcta tcagcaaaaa caataaccca
    11941 atgcgttttt tccctcgcat agattcctct aattaaattc cagaacaccg aaatcattgt
    12001 ataaataaat acacaacgaa ttgactactt gcttaattcg gcacaacact gcctgaaata
    12061 atcgctaaag aatatgattt attacaatgt
//
LOCUS       DAZKIF010000128        11722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZKIF010000128 DAZKIF010000000
VERSION     DAZKIF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.129"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..535
                     /locus_tag="ACHOTW_003795"
     CDS             <1..535
                     /locus_tag="ACHOTW_003795"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9845819.1"
                     /translation="NLSNRTLVKVPYLRVANVQRGYLDLTEIKQIEIPIEEKEKYQVV
                     KGDLLITEGGDWDTVGRTTVWCHDWYIANQNHVFKGRNIGQDVDPYWLETYMNSPFSR
                     QYFANASKQTTNLASINKTQLRGCPVAIPPSSEAKKIMSKLHIFYKLCEELKNHIQSA
                     QQTQLHLADALTDAAVN"
     gene            756..1097
                     /gene="symE"
                     /locus_tag="ACHOTW_003796"
     CDS             756..1097
                     /gene="symE"
                     /locus_tag="ACHOTW_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFP9845820.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(1144..2307)
                     /locus_tag="ACHOTW_003797"
     CDS             complement(1144..2307)
                     /locus_tag="ACHOTW_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFP9845821.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(2355..3236)
                     /locus_tag="ACHOTW_003798"
     CDS             complement(2355..3236)
                     /locus_tag="ACHOTW_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP9845822.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            complement(3333..3497)
                     /locus_tag="ACHOTW_003799"
     CDS             complement(3333..3497)
                     /locus_tag="ACHOTW_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP9845823.1"
                     /translation="MEFHENKAKTPFIRLVQLRQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            3674..5086
                     /locus_tag="ACHOTW_003800"
     CDS             3674..5086
                     /locus_tag="ACHOTW_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9845824.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(5329..6261)
                     /locus_tag="ACHOTW_003801"
     CDS             complement(5329..6261)
                     /locus_tag="ACHOTW_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9845825.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYNAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEERFNEFISELTSRMPQHRERIMTIAERIHNDGWLLGREEGEQRILRLLLQNGA
                     DPEWIQKITGLSAEQMQALRQPLPERERYSWLKS"
     gene            6728..7960
                     /gene="mdtM"
                     /locus_tag="ACHOTW_003802"
     CDS             6728..7960
                     /gene="mdtM"
                     /locus_tag="ACHOTW_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFP9845826.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            8001..9272
                     /locus_tag="ACHOTW_003803"
     CDS             8001..9272
                     /locus_tag="ACHOTW_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP9845827.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            9388..10539
                     /locus_tag="ACHOTW_003804"
     CDS             9388..10539
                     /locus_tag="ACHOTW_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP9845828.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            10549..11316
                     /gene="yjiL"
                     /locus_tag="ACHOTW_003805"
     CDS             10549..11316
                     /gene="yjiL"
                     /locus_tag="ACHOTW_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP9845829.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            11313..11579
                     /locus_tag="ACHOTW_003806"
     CDS             11313..11579
                     /locus_tag="ACHOTW_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP9845830.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
BASE COUNT         2843 a         2931 c         2884 g         3064 t
ORIGIN      
        1 aaatttatca aatagaactt tggtaaaagt tccttattta cgtgttgcaa acgtccaacg
       61 cggatatctt gatcttacgg aaattaaaca gattgaaatc cctattgagg aaaaagaaaa
      121 atatcaagta gtcaagggag atttattgat aacagaaggc ggcgactggg atacagtcgg
      181 gagaactaca gtatggtgtc atgactggta tatagcaaat caaaaccatg tattcaaagg
      241 acgaaatata gggcaagatg ttgatccata ttggttagaa acatatatga atagcccatt
      301 ctcaagacaa tattttgcta acgcaagtaa gcaaaccact aatttagctt ctattaataa
      361 aacccagctc agaggttgtc ctgttgctat tcctcctagc tcagaagcaa aaaaaataat
      421 gagtaaacta catatttttt ataaactatg tgaagaatta aagaatcata tccaatccgc
      481 ccagcaaacc caactacacc ttgcagatgc actcactgac gcggcggtaa actaatttac
      541 ctgccctcga aaaaccaata aagcggctaa atagccgctt tattcacatc accaaaattt
      601 atatttcacg ctaattttca tcttattttc attaattttt agctggactt tccccatatt
      661 tactgatgat atatccaggt atttagcgcg gtgcggatgt gcgccaacac acccgcaccg
      721 ctaatcacaa tcactattta tagggaatag cagttatgac tgatacgcat tctattgcac
      781 aaccgttcga agcagaagtc tccccggcaa ataaccgtca attaaccgtg agttatgcga
      841 gtcgttaccc ggattacagc cgtattcccg ccatcaccct gaaaggtcag tggctggaag
      901 ccgccggttt tgccaccggc acggtggtag atgtcaaagt gatggaaggc tgtattgtcc
      961 tcaccgccca accacccgcc gccgccgaga gcgaactgat gcagtcgctg cgccaggtgt
     1021 gcaagctgtc ggcgcgtaaa caaaggcagg tgcaggagtt tattggggtg attgcgggta
     1081 aacagaaagt tgcctgatac attgacccgg tagcgccagg actaccgggc atttttcgct
     1141 gcgttatacc aacgcgctct ccatctgttt ttcaaatgcc tgacgaatta accccataat
     1201 gtaaggaagt tgtgcgaggt cactgaaacc aatttccaca tcaccattgc cccatcgccc
     1261 aacatttgtc acatctttgg caataccttt cggatcgacc agttcatgaa actgcatatt
     1321 aagcgacaat cgcagtcggc ttttttgcgg cacaacatca acaaaattcg tttccgcttt
     1381 aaacgcaata tagagcttca atacttcctg cgtgatccct gcgtccaggc gcataatttc
     1441 atcgcgaaga tgatcgaata acacccggct atgcgaacca tcagcaagaa aaggataatc
     1501 actcaggctg taactggttt tgttctcagg ctgtccccga taaacagcta aaacctcttc
     1561 aggaagagaa ggcgcttgcc agacctgaac cgcaagctcc gccagacgat cggctcgggc
     1621 gtgaatggca gcttcatccc atttttcgca ctgtccaaga ccgatattca aatagagcgg
     1681 gctatgctta aagccgtctt caatatcgcg tttttctgcg aaaggtctgt cactgtaacg
     1741 ggagttatag cgcgttaaag tgagattccc caacgtatgc aacagttctt tatgaatacg
     1801 ctgccagtca cttcccagct cttcgcgcca tttagccgac aaattttcgt tctgcggcat
     1861 aatatgttca atcgtaaact cttccacgcg ctctctgcgt ttatcgtttt ccagtcgtcg
     1921 taaccagtag ctacgactac ggaagttata gagatcgcga acttttaatt cccgtttaaa
     1981 ttcatcatcg ttggggaaac gacgatatga aggtagattc aaaaaatgta cctgaatact
     2041 ttccagatat ttttctttat taatgacctt ataaaaagta gcaaacgtct tgttaagaga
     2101 atttgtcgga atcgcacata ctgcacggcg gaaaacataa gattcaatta aacgaattat
     2161 gctcaggaaa tcttcgtgag acaaatcgtc atttttatag tcatgataaa gcgccagtaa
     2221 gaaaggatac gccacatcaa cctttaactc gcgcaaatcc tgaaaagccg tagcaagcga
     2281 tttgtcactt tcttttccca atgccattgc acagtaatac tccgcgtaga tatgaatatc
     2341 ttcaaccagc cgatctacgc ctttttcagc aacactctgg ctgcgggcat ggagtttaaa
     2401 tgcctcatag acttcatctg tccgagggat ctccccgttt ttaccgtcag ataatgacgc
     2461 ataaaactgt caaaatattc gctgtaacct tgctgaccac aagcgacttc cattggacgc
     2521 cagtgatctt catacaaccg ggtttgatgc tctggttcga ggcccatcag aataaaattg
     2581 cgcaccagat cggcctgact taacgcctta ccggtggagt tcatactttc aaaaatcagt
     2641 tgcggattat cctgaccacg attaagcgcc acatcgacaa ttaataactt tgctaaccca
     2701 cgacataagg ggatcaagtc atcaccgagt ttggcaattt gttcatcaaa gaaagtaaag
     2761 ttttccatta ttcgatggga atagttttct ggcattggtc tttgttttat taacgccagt
     2821 aaactgtctt tatcagtctc ggtcagcagt aatttaaaac ctttctcgcc ggactcataa
     2881 ggattgagca aataataatt acgcaatttc attgccgaaa agccatcaaa aacttcgtct
     2941 tcgccaagat ggcgcgataa agcctcaatc agcaacattg cggtcgtcag ccgctgctga
     3001 ccatcaatga ccagcaacgg agaaatacca gaaacctgat acaatccctg ctctatataa
     3061 acaaccgaac cgataaaatg cgctgatata tcgtcacgct ttcctgcacg aatgatgtcg
     3121 tcccaaagtt gccgacattg ttgttctgtc catgaataag tccgttgata aatgggaata
     3181 acaaactgct gcgaacgttt taaaaaatcg agtaggcgag cttctgtagc tttcattctg
     3241 acgtctttcc gtgaaacgat gatttcgcaa taataaccac ttccttatgg aggttataga
     3301 gaaatcctgt ttatccgcga cgaacatacc cctcactcca catcctccct gcgtaaccca
     3361 atatccctca accgttcatc actcatctgc tgtaacaccc gtcgggcctg catttgccgc
     3421 cgccaacgcc tcactgcctg cctgagttgc acaagtctga taaacggcgt tttggctttg
     3481 ttttcgtgaa attccatctg ctgcctcctc atatttcaga ggcttcatca tccgtcttct
     3541 ggcagttcac aatacagatg caaatattag ttttgtttaa catacagaca ggttaaaaca
     3601 gcatctgcat ccggtttttt agcctcatct gtaccggttt ttctttctgt atggtccaaa
     3661 taaggaatac agcatgacgc gttatcaaca tctggcgact ctacttgccg agcggattga
     3721 gcaagggctg tatcgtcacg gggagaaatt gccgtcggtg cgcagcttaa gtcaggagca
     3781 cggcgtcagc atcagcaccg tgcagcaggc gtaccagacg ctggagacga tgaagctcat
     3841 cactccgcag ccgcgttcgg gttattttgt cgcacaacgt aaagcccagc cgccagtgcc
     3901 gccgatgacg cgtccggtgc agcgcccagt ggaaattacc cagtgggatc tggtgctgga
     3961 tatgctggtg gcgcatagcg acagttccat tgttccgtta agcaaaagca cgccggatgt
     4021 cgaaacgccc agcctgaaac cgctgtggcg ggagctaagc cgggtggtgc agcataatct
     4081 gcaaaccgtt ctcggttatg acttgctagc cggtcagcgg gtattgcgcg agcaggttgc
     4141 ccgcctgatg ctcgacagcg gctcggtggt cactgccgat gacatcatca tcaccagcgg
     4201 ctgccataac tcgatgtcgc tagcgttaat ggcggtgtgt aaaccgggcg atattgtcgc
     4261 ggtcgaatcc ccctgttatt acggttcaat gcagatgctg cgcggcatgg gcgtgaaagt
     4321 gattgaaatc ccaaccgatc cagaaacagg catcagcgtt gaagcgctgg aactggcgct
     4381 ggaacagtgg ccgattaaag gcatcattct ggtgccaaac tgtaataatc cgctgggatt
     4441 tattatgccg gacgcgcgca aacgggccgt tctctctctc gctcagcgtc atgatattgt
     4501 gatttttgaa gatgatgtct acggcgaact ggcaacggag tatccgcgcc cgcggaccat
     4561 tcattcctgg gatatcgacg ggcgagtgct gttgtgcagc tcgttcagta aaagtattgc
     4621 tccaggcctg cgcgtgggtt gggtcgcacc ggggcgttat cacgataaac tgatgcatat
     4681 gaaatacgcc atcagcagct ttaatgtgcc gtccacgcaa atggcggcgg caacgtttgt
     4741 gctggaaggt cactatcatc gccatatccg gcggatgcgg cagatctatc agcgcaattt
     4801 ggcgctttat acctgctgga tacgggaata ttttccctgc gaaatctgta ttacgcgccc
     4861 gaaaggcgga tttttactgt ggatagaatt gcctgaacag gtcgatatgg tctgcgtcac
     4921 gcggcagctg tgccgcatga aaatccaggt ggcggcaggc tcgattttct cagcttccgg
     4981 caaataccgt aactgcctgc gcatcaactg tgctttgcca ctcagcgaaa cttatcgcga
     5041 agcgctgaag caaattggcg aggccgtgta tcgggcaatg gaataaatta gaattagtgg
     5101 cgaagcacct gatttattct cacgaatcgc ctgtgactcg ttttatcttt gcaggatcat
     5161 agccacgttc cggcatggct taggtaaagt gcgctttgtt catgccggat gccatgtgaa
     5221 cgccttacca ggtctacaaa atctttaaag ttcaatatat tgaatgggct ttgtagacct
     5281 gataagcgta gcacatcagg ctgctttgcg tttgtcatcc gtctctggtt agctcttgag
     5341 ccatgaatag cgctcacgct caggcaaggg ctgccttaat gcctgcattt gctctgccga
     5401 aagtccggta atcttctgta tccattcagg atctgcccca ttctgcaaca acaatcgaag
     5461 aatgcgctgt tccccttctt cccttcccaa cagccatcca tcattatgaa ttcgctctgc
     5521 aatcgtcatt attcgctctc tgtgttgagg cattcgactg gttagttcac tgataaactc
     5581 attaaaacgc tcttcatcgc cagtcagtaa aatgtaattt aacagcgcag ttatctggct
     5641 gtcattagca cactctgtaa ccagtaatac taccagttga tcgataagcc ccatcagatc
     5701 gcgctggcga atatgctttt ggatcaactc caacagggcg actctgcggt gctgcacaat
     5761 ctcgtcgtcc ggcacgacag tgatatccac cagcgggaac gcggcgttat aaagcttccg
     5821 tgcagtagtc gggtcggcaa attcgtccaa ccagcacaaa gaccagggat aaggactacg
     5881 gctaccgtga taaaacagca tcgggatgac cagcggtagt ggccggcgtt tatcatgctc
     5941 tatatggcgc tgcatcactg ccatggaata gcgcatcagg cgaaaggcca tatgaatatc
     6001 ctcacggctc tgatgttcaa tcaccacata gatatagcca tcgccttccc gggtctttac
     6061 cgaccacaga atatcggagt gtagcgcccg caatttttca tcgacaaagc tggcggattc
     6121 cagttttaag ctgtcgagat cgcacagttc acgtaaatct ttgggtaagt ggatctccat
     6181 aaaatcccgc gcggtgtcag ggtgcatgag aaaagattta aataatgcgt catgcggcgt
     6241 gctggtcgtg aagtttgtca tggcgatccg tcacctgttg ataagatggc ccgactttag
     6301 cgactaacaa aaccattctc acccggcaat ttctcgtttc ccgaggcgct ttccaggcta
     6361 tttttgttcg tctgcaaacg atacattttt ccggaaactg actcgcgttt tacatgtcct
     6421 gtacccggca gatattcccc cttccaccgc gctcttttga ttaatcgcaa attttaatta
     6481 cacttaaggt gtatattttc tatgcaaccc atcaattcaa gaggtgtaat gtgctgatga
     6541 ctatttgtaa tcgttataca ttctgacccg aagtcagaag gtatttctct gtctgtgtgt
     6601 tcacaggcag tgtggttgat tacatgaaat tcagtacatt tgtccttctc gcctaccttt
     6661 tgtcattacc gacggtattg aatttcgttt tccccgttgg ggttctccgg acaaggagtt
     6721 gtttgttatg ccacgttttt ttgcccgcca tgccgccacg ctgtttttcc cgatggcgtt
     6781 gattttgtat gactttgccg cctatctttc gacggacctg atccagcctg ggatcattaa
     6841 tgtggtccgt gattttaatg ccgatgtcag tctggctccg gctgccgtca gtctctatct
     6901 cgctggaggc atggcgttgc agtggctgct ggggccgctt tccgacagga ttggccgcaa
     6961 gccagtgctg attaccgggg cgctaatatt tacccttgcc tgcgctgcga caatgttcac
     7021 aacgtctatg acacagtttc ttatcgcgcg tgcaattcag ggcaccagta tctgttttat
     7081 tgccaccgtt ggttatgtca cggtgcagga ggcgttcggc cagacaaaag ggatcaagtt
     7141 gatggcgatt atcacctcca tcgtactgat tgcgccgatt atcggcccgc tttccggcgc
     7201 agctctgatg cactttgtgc actggaaagt cctttttgcc atcattgcgg ttatgggttt
     7261 tatctcattt gttggcttac tgctggcgat gccagagacg gtgaagcgcg gcgcggttcc
     7321 atttagcgcc aaaagcgtct tgcgcgattt tcgtaatgtc ttttgcaatc ggctgttcct
     7381 ctttggcgca gcaaccatct ctttaagcta tatccccatg atgagttggg tggctgtctc
     7441 gccggtgatc ctgattgacg caggcggctt aacgacctcg cagttcgcct ggacacaggt
     7501 tccggtgttc ggcgcggtga ttgtcgcgaa tgccatcgtg gcgcgtttcg ttaaagatcc
     7561 gaccgaaccg cgctttatct ggcgtgccgt acccattcaa ctggtcggcc tcgcgctgtt
     7621 gattatcggc aatctgctgt caccgcacgt ctggctgtgg tcggtgctgg gcaccagtct
     7681 gtatgctttc gggattggtt tgatcttccc gaccttattc cgctttacgc tgttttccaa
     7741 taacttaccg aaagggaccg tctccgcatc gctgaatatg gtgatcctga tggtgatgtc
     7801 tgtctcggtc gaaattggcc gctggctgtg gtttaacggc ggtcgcttgc cgtttcatct
     7861 gttagccgtt gtggcgggcg ttatcgtcgt tttcaccctg gccggattgc ttaatcgcgt
     7921 gcgccaacat caggctgccg agctggcaga ggagcagtga tttttgcgcg atcctgccgt
     7981 caggctctat tcttaacgtt atgaataaac tcattgaact cagacgcgcc aaaatgttgg
     8041 cgctctcttt actgcttatc gccgctgcta cctttgtcgt tacgctgttt ttgccgccca
     8101 atttttgggt gagcggcgtg aaggcgattg ctgaagcggc gatggtcggc gcgctggcgg
     8161 actggtttgc ggtggtggcg ctgtttcgcc gcgtgccgat tccgatcatt tctcgtcata
     8221 cggcgattat cccgcgtaat aaagaccgga ttggcgaaaa tctcggtcag ttcgtgcaga
     8281 aaaaatttct cgatacccaa tcgctggtgg cattgattcg acgtcacgaa ccggcgttgc
     8341 tgattggcaa ctggtttagc cagccagaaa acgcccgccg cgttggtcag catctgttgc
     8401 agatcatgag tggttttctt gaactgaccg atgatgcacg tattcagcgc ctgcttaagc
     8461 gcgcggtcca tcgggcgatt gataaggtcg atctttccgg caccagtgcg ttgatgctgg
     8521 agagtatgac caaaaacgat cgtcatcagg tgctgctgga tacgctgatc gcacagttga
     8581 tcgcccttct ccagcgcgat aaatcgcgca agtttatcgc ccagcagatt gttcgctggc
     8641 tggagagtga gcatccactg aaagccaaaa ttctccccac cgaatggctg ggcgaacata
     8701 gcgcggagtt ggtttctgac gcggtgaatt ctttgcttga tgatatcagc cgcgatcgtg
     8761 cgcatcagat ccgccatgca tttgatcgcg ccacttttgc cctgatcgac aagttgaaaa
     8821 acgatcctga aatgacagcg cgagctgatg ccgtaaaaag ctatctgaaa gaggatgaag
     8881 cctttctcag tgaattgtgg ggggatttac gggaatggct gaaagcggat atcaacagtg
     8941 aagattctcg tgtgaaagaa cgtatcgcgc gggcaggtca atggtttggc gaaacgttaa
     9001 ttgccgatga tgccttgcgg gcgtcgttaa atggtcacct ggaacaagcc gcgcaccgcg
     9061 tcgcgcctga gttttctgca ttcctgacgc gccatatcag cgatacggta aaaagctggg
     9121 atgcccggga tatgtcgcgg caaatagagt taaatatcgg caaagatctg cagtttatcc
     9181 gtgtcaacgg tacgctggtt ggcggttgta ttgggctaat tttatatttg ctgtcacagc
     9241 tcccggcctt gttccccctc ggcaattttt agaaatccat gaaaaactaa gtggctaacc
     9301 gcgagagaga tcaaataaac catactaaaa gaatggtaat aatgtggcct cttttcatta
     9361 tctcccgtgg tacggggaag gaaaatcatg tcacttgtca ccgatctacc cgccattttc
     9421 gatcagttct ctgaagctcg ccagaaaggc tttctcaccg tcatggatct caaggtgcgc
     9481 ggcattccgc tggttggcac ttactgcacc tttatgccgc aagagatccc gatggcagcc
     9541 ggtgcggttg tggtttcgct ctgttccacc tctgatgaaa ccattgaaga agcggagaaa
     9601 gatctgccgc gcaacctctg cccgctgatt aaaagtagct acggcttcgg caaaaccgat
     9661 aaatgcccct acttctactt ttcggatctg gtggtcggtg aaaccacctg cgacggcaaa
     9721 aagaaaatgt atgaatacat ggcggagttt aagcccgttc atgtgatgca gctgccaaac
     9781 agcgttaaag acgatgcctc gcgtgcgtta tggaaagccg agatgctgcg cttacaaaaa
     9841 gcggtggaag aacgttttgg gcacgaaatt agcgaagatg ctctgcgcga tgccattgcg
     9901 ctgaaaaacc gcgaacgtcg cgcactggcc aatttttatc atcttgggca gttcaatcct
     9961 ccggcgctta gcggcagcga cattctgaaa gtggtttacg gcgcaacctt ccggttcgat
    10021 aaagaggcgt tgatcaatga actggacgcg atgaccgccc gcattcgtca gcagtgggaa
    10081 gaaggccagc gactggaccc gcgtccgcgc attttaatca ccggctgccc gattggcggc
    10141 gcagcagaga aagtggtgcg cgcgattgaa gagaatggcg gctgggttgt cggttatgaa
    10201 aactgcaccg gggcgaaagc gaccgagcaa tgcgtggcag aaacgggcga tgtctacgac
    10261 gcgctggcgg ataaatatct ggcgattggc tgctcctgtg tttcgccgaa cgatcagcgc
    10321 ctgaaaatgc tcagccagat ggtggaagaa tatcaggtcg atggcgtagt tgatgtgatt
    10381 ttgcaggcgt gccataccta cgcggtggaa tcgctggcaa ttaaacgtca tgtgcgtcag
    10441 cagcacaaca ttccttatat cgctattgaa acagactact ccacctcgga tgttgggcag
    10501 ctcagtaccc gtgtcgcggc ctttattgag atgctgtaag gagtagcagt gacatattcg
    10561 attggcattg attctggttc taccgccacc aaagggatct tactcgcaga cggcgtgatt
    10621 acgcgccgtt tcctcgttcc aacacccttt cgaccggcaa cagcaattac tgaagcctgg
    10681 gaaactctgc gcgaagggtt agagacaacg ccgtttctga cgctcaccgg ctacgggcgg
    10741 caactggtgg attttgccga taaacaagtg acggaaatct cctgtcacgg gctgggcgca
    10801 cggtttcttg cgccagcaac gcgcgcggta atcgacatcg gtggtcagga cagcaaagtg
    10861 attcagcttg atgatgacgg taacctgtgc gatttcctga tgaatgacaa atgcgcggcg
    10921 ggcaccgggc gtttcctgga ggtgatctcg cgcacgcttg gcaccagcgt cgagcaactc
    10981 gactgcatta ccgaaaatgt cacgccgcac gccatcacga gtatgtgcac agtgtttgct
    11041 gaatcagaag tgatcagcct gcgctcagcg ggcgtcgcgc cagaagcgat tctcgccgga
    11101 gtgattaacg cgatggcgcg gcggagtgcc aatttcattg ctcgtctctc ctgtgaagcg
    11161 ccgattctgt ttactggtgg cgttagtcat tgccagacgt ttgcccggat gctggaatct
    11221 cacctgcgaa tgccggtaaa tacccatcct gatgcgcaat ttgctggcac aattggcgcg
    11281 gcggtaattg gtcaacgagt gaggacacgc cgatgaaaga gtttttattt ttatttcact
    11341 ccacggtcgg cgtcatacaa acccgcaagg cgttgcaggc agcgggcatg acttttcgcg
    11401 tcagcgatat tccccgtgat ttacgcggcg gctgcgggtt atgcatttgg ctgacctgtc
    11461 cccccggcga ggaaatacaa tgggtgatcc ctgggcaaac cgaagcgata tattgccagc
    11521 agggcagcga ctggcagtgc gtggtgcatt acgatgcaga agcgtcaacc aggcaatagg
    11581 atctttaaat ataccaaccg gataattggc tgcgcataat cctgcatgaa ggaagttgta
    11641 gtaaatatct cgcctgccgt gaaatatttc ccatccacaa aatgagcgat ttttgatagc
    11701 accttcctat tgcgcgtcta aa
//
LOCUS       DAZKIF010000129        11631 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZKIF010000129 DAZKIF010000000
VERSION     DAZKIF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.130"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            200..802
                     /gene="lpfA2"
                     /locus_tag="ACHOTW_003807"
     CDS             200..802
                     /gene="lpfA2"
                     /locus_tag="ACHOTW_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFP9845831.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
     gene            963..1289
                     /locus_tag="ACHOTW_003808"
     CDS             963..1289
                     /locus_tag="ACHOTW_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9845832.1"
                     /translation="MATAGIVVGGTRVIFYGDKPDTTISIFNKETNLPYLLQTWVDPF
                     NKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESVFWLNVKNIPPSIDKK
                     IPMFLR"
     gene            1442..1591
                     /locus_tag="ACHOTW_003809"
     CDS             1442..1591
                     /locus_tag="ACHOTW_003809"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9845833.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            1613..4147
                     /locus_tag="ACHOTW_003810"
     CDS             1613..4147
                     /locus_tag="ACHOTW_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9845834.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            4159..5229
                     /locus_tag="ACHOTW_003811"
     CDS             4159..5229
                     /locus_tag="ACHOTW_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9845835.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            5257..6339
                     /locus_tag="ACHOTW_003812"
     CDS             5257..6339
                     /locus_tag="ACHOTW_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9845836.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            6551..7591
                     /gene="pstS"
                     /locus_tag="ACHOTW_003813"
     CDS             6551..7591
                     /gene="pstS"
                     /locus_tag="ACHOTW_003813"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9845837.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            7678..8637
                     /gene="pstC"
                     /locus_tag="ACHOTW_003814"
     CDS             7678..8637
                     /gene="pstC"
                     /locus_tag="ACHOTW_003814"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP9845838.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            8637..9527
                     /gene="pstA"
                     /locus_tag="ACHOTW_003815"
     CDS             8637..9527
                     /gene="pstA"
                     /locus_tag="ACHOTW_003815"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9845839.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            9710..10483
                     /gene="pstB"
                     /locus_tag="ACHOTW_003816"
     CDS             9710..10483
                     /gene="pstB"
                     /locus_tag="ACHOTW_003816"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9845840.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            10498..11223
                     /gene="phoU"
                     /locus_tag="ACHOTW_003817"
     CDS             10498..11223
                     /gene="phoU"
                     /locus_tag="ACHOTW_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9845841.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         3036 a         2702 c         2876 g         3017 t
ORIGIN      
        1 ttaagcaatc acataacgat ataaatttag ttttgcttaa cacctaaatt aatttatcta
       61 aatgtataaa ataaaaacca cttgcgccaa agtatattgg tagtactatt tggtttctga
      121 tcaaataccg atcaggttta tccaaattta ataaatggat aacacacagg aataatatca
      181 aattaatgga gttatattta tgaaaccaaa tatgattgta ggagcattag cgttaacttc
      241 tgtgtttatg gcaggtcacc tacaggcggc tgatggaaca gtccatttcc gtggtgaaat
      301 tattgacagt acttgcgaag tcactcctga aactaaagat caggtcgttg atttaggcaa
      361 agtaaaccgt acagccttta gtggcgtcga tgatgtggct gccccgacgg ctttttctat
      421 cgatctgact caatgcccgg aaacctttaa gtccgccgca attcgtttcg atggtaatga
      481 agatgctcat ggtaatggca acctggcaat tggtaccccg ctggataact ctaacgatgc
      541 tgccgctggt attagcccga gtgataacag tggggattat actggtgcgg gtgccgttag
      601 tgcagcgaaa ggcgtagcta ttcgtttata taaccgtgca gataacactc aggtcaagtt
      661 atatgaaaat tctgcatcaa ctccgatttc taatggtaat gcatccatga agttcatggc
      721 tcgttatatt gctacggaaa cgactattga ccctggtaca gctaacgccg actcgcagtt
      781 tacagttgaa tatataaaat aaatcattct taacataata ccttaaataa aatggcgctc
      841 gccattatgg ttctctttta cttaaatgaa gggcgtcatt attatatttt ttatgagata
      901 catttatgcc tatgtattta cttagattgt tttttgctgt tactttgatt acgcagatgt
      961 cgatggctac ggctggtatt gttgttgggg gaactcgcgt tattttttat ggtgacaaac
     1021 ctgataccac aatctctatt tttaataaag aaacgaacct tccttatcta ttgcagacat
     1081 gggttgatcc atttaataaa gaggataaaa gcaaaccgcc attttctgtg atacctcccg
     1141 tttctcgcat tgaaccacag caagagaaaa tattacgcat tttaaaaacg gctggcgagt
     1201 taccaaaaga tcgggagtcc gtattctggt tgaacgtgaa aaacatccct ccttcaattg
     1261 ataaaaaaat accaatgttc ttgagatagc cataaaaaca agaattaagt tattctggcg
     1321 accagccgcg gtaaagataa tccctgagca ggctattaaa aatgttaaat ggtcgcggca
     1381 gggtaataat ctcatcgtcg aaaatccaaa tccgatccat attaatgtaa tgagtgtgac
     1441 ggtggatgat aaagacgtgt cgctaaatat gatccgccct tttgaaatat tgacattacc
     1501 aattccggct ggcgtggcag gaaaatcgct cgtttggcgc tttattaatg attacggtgc
     1561 aattagtcaa ccactaaaga aaaaccttta agcgccggat aaggatatat cgatggcctc
     1621 ttcgcatctt ttcattacgc ttgcatcggg catatgtctg ctctgttcca tatctgcttt
     1681 tgcccgggat agcttgttca accccagatt actggaactg gatcatcctg cggataatat
     1741 tgatattcac cagttcaacc gttcgaatac cttacctgcg ggaacataca aagttgatgt
     1801 gatgatcaac ggcatgctct tcgaacgcca ggaagttaaa ttcgcccagg ataaccctga
     1861 tgctgaactc cacccatgct acgtggcgat aaaaaacgtg ctggcgacct atggtataaa
     1921 agttgatgcg ataaaatctc tggcgaatgt tgatgacaaa acatgcgtaa atccagttcc
     1981 gctgatcgac ggggctacct ggttactgga cgccagtaaa cttgcattga atattactat
     2041 tccgcaaatt tatctcaaca atgcagttaa tggttatatc agcccttccc gttgggatca
     2101 ggggatcaat gccatgatga tgaattatga tttttcggca tcgcatacca tccggtcaaa
     2161 ttatgacgac gacgatgaca gttattatct gaatttgcgt aatggtatta atttaggcgc
     2221 atggcgtttt cgtaattaca gcaccctgaa ttcttatgac ggtaatgtgg actaccattc
     2281 cgtcagtaat tacattcagc gcgacatcat ggcattacgt agccagatta tgattggcga
     2341 tacctggacg gcaagcgatg tatttgatag tacacaggtg cgtggcgtgc ggctgtatac
     2401 cgatgacgat atgttgccct ccagccagaa cggctttgcg ccagtggtac atgggattgc
     2461 gaaaactaac gccacggtga tcatcaaaca aaacggctac gttatttatc aatcagccgt
     2521 accacagggc gcatttgccc tcaccgactt aaacacgacc agtagcggcg gcgatctcga
     2581 tgtcactatc aaagaagaag atggcagcga gcagcacttt attcagccat ttacttcact
     2641 ggccattctc aagcgtgaag gtcagaccga tgtagacctt agcattggag aagtgcgcga
     2701 cgaaagcggc tttacgcctg aggtcttgca gttacaagca atgcacggtt tccctttggg
     2761 aataactttg tatggcggaa cacaattggc aaatgattac gcttctgccg cgctgggtat
     2821 tggtaaagat atgggggcgc tgggcgcgat ttcttttgac gtgactcatg cccgctcgca
     2881 gtttgactac gacgataatg agagtggtca atcgtatcgt tttctctatt ccaaacgttt
     2941 tgaagacacc aataccacct ttcgtctggt gggttatcgc tactctatgg aggggttcta
     3001 caccctcaat gaatgggtgt cgcgacagga taatgattct gatttctggg taacgggcaa
     3061 ccgtcgcagc cgcttcgaag gcacctggac gcaatctttc acgccaggct ggggcaatat
     3121 ttatttaaca ttcagtcgac aggaatactg gcagaccgat gaggtcgaac gtttattaca
     3181 gttcggctat aacaacaact ggcgaaacat ctcctggaac gtttcctgga actatacgga
     3241 ctcgatcaag cgctcattgg gcaaccatca tgatgataac aatgatgatt tcggcaaaga
     3301 acagattttc atgttctcaa tgtcgatacc gctatcgtgc tggatggaag acagctacgt
     3361 caactattcg ttaacgcaaa acaaccacca tgaaagcacg atgcaggtcg gtctgaacgg
     3421 aacgatgctg gaagggcgta acctgtctta taacgtacag gaatcgtgga tgcactctcc
     3481 tgatgactcc tacagcggca atgccggaat gacctatgac gggacttatg gctcggtcaa
     3541 tggtagctat tcctggagcc gtgactccca acattttgat tatggcgcca gaggcggcgt
     3601 gctggtgcat agtgacggag tgaccttctc gcaggaactg ggcgaaacgg tggcattggt
     3661 caaagcgccg ggcgcagaag gcctgtccat tgaaaacgcc accgggattt ctaccgactg
     3721 gcgtggttat accgtaaaaa cgcagcttag cccgtatgac gaaaaccgcg tggcattgaa
     3781 cagcgactat ttctccaaag ccaatattga actggaaaac accgtcatca acctggtacc
     3841 aacgcgcggt gcggtggtga aagccgaatt tgtcacccat gtcggttatc gcgtgctatt
     3901 taacgtccgc caggtcaacg gtaaaccaat aatgtttggc gcgatggcaa ccgcctctct
     3961 cgaaacgggc acagtcaccg ggattgtcgg tgataacggc gaactgtatc tctccgggat
     4021 gcctgaaaaa ggcgagtttt tattgagttg gggacaagct gcggatgaaa aatgtaaggc
     4081 ggcctatcac atcacccata aacctgatga taccagcctg gttcaaatgg atgcgatttg
     4141 tcgctaaagg aagcatgaat gaaaaaaata ttatcagggt tgattctgct gctttgctgt
     4201 ccttatggtt tcgccgctaa tggtgatggc gcaacgcaca tgtcaaattt atcatttggt
     4261 ccgctgacgg tggcagcggc gaataaccac tccggataca atattttcga ggcactgagc
     4321 aacacgactg gaacataccc ggtgcgctgt cactgtgatg acacgcatgg cggaccgggc
     4381 caacaaacag cattttttcc tatcttctac acgggagatg ccgcaccggg gcttgtgctt
     4441 gagcgcactc ttaatgggtt aaattactat gctctgaatg attatttatc ggtcggcgtg
     4501 acgattttta ttattaataa ccaatatgcg gccattcctt ttgaacactt atccaaccaa
     4561 tccacctcac cgcaacatac ctgtggagca ggtaataatg ggagcactgt aaatctggat
     4621 tcagggcgct cggcaaaatt atctttctat gttcggcatt ctattactgg cacggtgaca
     4681 atacccacaa cggaagtcgc ctggttgtac gcgggcatgt ccgatcattt tcccaaaacg
     4741 acccccgttt ctaaagtgac aattcgcgga caactaacgg ccccgcagaa ctgtgagtta
     4801 acgccaaatc agagcatcga tgtcgatttt caaaaaatta atagcgctga gttctcctca
     4861 acggcgggtt caattattgc agaaagaaag attaaaaccg aagtcacagt atcctgtacc
     4921 gggatggaag acgtaaggtc cacggaggtg gtgagtgcgt cgatgattgc ggcaaacaga
     4981 agtgccgatg ccaccatgat cgtgacgagt aatccggatg tgggaattaa gatttttgat
     5041 aagaacgacc gtccagtgaa tgtggatggg ggcaacttac ccgctgatat gggtgctatt
     5101 agtcgattag gaaaaaccga tggtagcgta acgttttatt cagcgcccgc cagtctgacg
     5161 ggcgcaaaac cagcgcctga taatggattt accgctacag ccacgctggt tattgaattt
     5221 actaactaat cttgtgttag taaaaggaat aatttcatga aaaaatggca ttatatattt
     5281 tgcataattc tctttcattt agggttaccg tgcgggtatg cggcaaatga tggcacgtgt
     5341 gcaacaagag gcggcacaca tacattaagc cttaattttc ctctgacaac ggtcagtgca
     5401 gcaaacaatg tgcctggaaa tacattaata gatattgcta atgcaacatc ttctgaaaat
     5461 tatagcgttc tgtgtaactg tgattcaaaa catagcaatg gcgcttatca cgaaatatat
     5521 tataccgcag accctgctcc cggtatggtt tatagcacca ccgcaagtgg tcttgctttt
     5581 tactatctta acgaatatgt cgatgtggga acaaaaatat ctgtgctaaa tgcggggtat
     5641 acggcagttc cttttgaaca tgtttccaac caggcaacta caacagatca cacttgtcag
     5701 ggaaacaaaa ctacagcggt tggcgtgagc ctgaaaactg gagcagatgc gaagatttca
     5761 tttcgtatta aacgttcaat aaatggaacg gtagtaatac ctatcaccga tattgcattg
     5821 ctgtatgcca acatatccag caccacgacc cgtggtgagg cgattgcaaa agttcgaatt
     5881 tcaggcagtt tgaccgcacc acagtcttgt cagataaatg caggacaggt gatttatttt
     5941 gattttgata ctattcctgc gtccgaattt tcatctaccg ccgggcaagc cattacttca
     6001 cgaaaaatca ctaaaacagt gagtattgag tgtacgggga tggggtatga gcgtacgcag
     6061 aaagtcgatg cttcttttac ggggacgaac cgaagcagtg acgatacgat ggtggcgaca
     6121 gacaatgctg atgtcgggat caaaatttac aataaatcga atgctgaagt tagcgtcaac
     6181 aacggcaagt tacccgcaga catgggcaac acgaccattt ttggtcgtaa aaatggttcg
     6241 gtaacttttt cggcagcacc tgccagcttt accggtgccc ggcctcagcc cggcgttttt
     6301 aacgctaccg cgaccttaac cattgaattt gtaaactaac cttttgcggc tgttttatga
     6361 cagccgttca tatttcctct cctttcatga aaaaatgctg atatttttcc ccttcaatat
     6421 caataagtaa taactttcct ttgggtgtca tcaaactgtc acattcctta catataactg
     6481 tcacctgttt gtcctatttt gcttctcgta gccaacaaac aatgctttat gaatcctccc
     6541 aggagacatt atgaaagtta tgcgtaccac cgtcgcaact gttgtcgccg cgaccttatc
     6601 gatgagtgct ttctctgtgt ttgcagaagc aagcctgaca ggtgcaggtg caaccttccc
     6661 tgcgccggtg tatgccaaat gggctgacac ttaccagaaa gaaaccggta ataaagttaa
     6721 ttaccagggt atcggttctt ccggtggcgt aaaacagatt atcgccaata ccgttgattt
     6781 tggtgcttct gacgcgccgc tgtctgacga aaaactggct caggaaggtc tgttccagtt
     6841 cccgaccgtg attggcggcg tggtgctggc ggttaacatt ccaggtctga agtctggtga
     6901 gctggtgctg gatggtaaaa ccctcggcga tatctacctg ggcaaaatca agaagtggga
     6961 tgatgaagcc atcgccaaat tgaatccggg tctgaaactg ccttcacaga acattgctgt
     7021 agtgcgccgc gcagatggct ccgggacttc cttcgtcttc accagctacc tggcgaaagt
     7081 gaatgaagag tggaaaaaca acgttggtac tggctctacc gtaaaatggc cgatcggtct
     7141 gggtggtaaa ggtaacgacg gtatcgctgc gttcgttcag cgtctgccgg gtgcaattgg
     7201 ttacgttgaa tatgcttatg cgaagcaaaa caacctggcg tacaccaaac tgatctccgc
     7261 tgatggtaaa cctgttttgc cgaccgaaga aaacttcgct aatgcagcaa aaggtgcaga
     7321 ctggagcaaa accttcgctc aggatctgac caaccagaaa ggcgaagatg cgtggcctat
     7381 tacctctacc acgttcattc tgatccacaa agatcagaag aaaccagaac aaggcacaga
     7441 agtgctgaaa ttcttcgact gggcgtacaa aaccggggct aaacaggcga acgacctgga
     7501 ttacgccagc ctgccggata gcgtagttga acaggttcgc gctgcgtgga agaccaatat
     7561 taaagacagt agcggcaagc cgctgtacta ataaaactcc aggccggata aggcattaaa
     7621 cggcttatcc ggcattacaa aatgactttg taaacgcgtt taactgaaga gtaacttatg
     7681 gctgcaacca agcctgcttt taacccaccg ggtaaaaagg gcgacataat tttcagcgtg
     7741 ctggtaaaac tggcggcgct gattgtgcta ttgatgttgg gtggcattat tgtctctctg
     7801 atcatctcct catggccgag cattcagaaa tttggtctgg ctttcctgtg gaccaaagag
     7861 tgggatgcgc cgaacgatat ctacggggcg ctggtgccga tctacggtac gttggtgact
     7921 tcgtttatcg cgctgctgat cgccgtcccg gtgagtttcg gtatcgccct gttcctgact
     7981 gagcttgcgc ctggctggct gaaacgcccg ctgggtatcg ccattgagct gctggcagcc
     8041 attccaagta tcgtttacgg catgtggggc ctgtttatct ttgcgccgct gttcgccgtt
     8101 tacttccagg agccggtcgg caatatcatg tcgaatatcc cgattgtcgg cgcgctgttc
     8161 tctggcccgg catttggtat cggtatcctc gcggcaggcg tgatcctcgc catcatgatt
     8221 attccgtaca ttgcggcggt aatgcgcgat gtgttcgaac aaaccccggt gatgatgaaa
     8281 gagtcggcct acggtattgg ctgcaccacc tgggaggtta tctggcgtat cgttcttccg
     8341 ttcaccaaaa atggtgttat cggcggcatt atgctggggc tgggccgcgc gcttggtgaa
     8401 accatggcgg tgacctttat tatcggtaac acctaccagc tcgacagcgc ctcgctgtac
     8461 atgccgggca acagtatcac ctctgcgctg gcgaacgaat ttgccgaagc ggaatccggt
     8521 ctgcacgttg ccgcactgat ggaactgggc ctgatcctgt ttgtgattac cttcatcgtc
     8581 ctcgccgcat cgaagtttat gattatgcgc ctggctaaga atgagggggc tcgctaatgg
     8641 ctatggttga aatgcaaacc actgcggcgc tggcggaatc ccgccgcaaa atgcaggcgc
     8701 gtcgccgcct caaaaaccgt attgcactga cgctctcaat ggcgacgatg gccttcggtc
     8761 tgttctggct gatctggatt ttaatgtcca ccatcacccg cggtatcgac ggtatgtcgc
     8821 tggcgctgtt cactgaaatg acgccgccgc ccaatacgga aggtggtggt ctggcgaacg
     8881 ctctggcagg tagcgggctg ttaattttgt gggccacggt attcggtacg ccgctgggca
     8941 ttatggcggg gatttatctg gctgaatatg gtcgtaaatc ctggctggca gaagtgattc
     9001 gcttcattaa cgacattctg ctctctgcgc cgtcgattgt ggttggtctg tttgtttaca
     9061 ccatcgtggt ggcgcagatg gagcacttct ccggttgggc gggtgtgatt gcgctggcgt
     9121 tgttgcaggt gccgattgtt atccgcacca ccgagaacat gctgaaactg gtgccggaca
     9181 gcctgcgtga agcggcttat gcgctgggta caccgaagtg gaagatgatc tccgcgatta
     9241 cgctgaaagc gtcggtgtcc gggatcatga ccggtatcct gctggcgatt gcccgtattg
     9301 ctggtgaaac cgcgccgctg ctgtttaccg cgctctccaa ccagttctgg agcacggaca
     9361 tgatgcagcc gatcgccaac ctgccggtga cgatctttaa gtttgcgatg agcccgtttg
     9421 cggaatggca gcaactggcc tgggccgggg tattgatcat taccctgtgc gtactgctgc
     9481 tgaacattct ggcgcgcgtt gttttcgcga agaataaaca cggttgatat tgctgacacg
     9541 gttttcccct caccctaacc ctctccccag aggggcgagg ggaccgaccg agcgcctttt
     9601 tgactctgta cacggttaac actttgccgg atgcggcgtg aacgcctgat ccggcctacg
     9661 gtaagcctga ttcgcgaagt gcatcaggca cgatgaggaa aagattgcaa tgagtatggt
     9721 tgaaactgcc ccgagtaaaa ttcaggttcg taatttgaac ttctactacg gcaaattcca
     9781 tgccctgaaa aacatcaacc tggatatcgc caaaaaccag gtaacggcgt ttatcgggcc
     9841 gtccggctgc ggtaaatcga cgctgctgcg taccttcaac aaaatgtttg aactgtaccc
     9901 ggaacagcgt gcggaaggtg aaattctgct cgatggcgac aacatcctga ccaactctca
     9961 ggatatcgcc ctgctgcgtg cgaaagtggg catggtgttc cagaaaccga cgccgttccc
    10021 gatgtccatc tacgacaaca tcgcctttgg cgttcgtctg tttgagaagc tctctcgtgc
    10081 cgacatggac gagcgcgtgc agtgggcatt gaccaaagcc gcattgtgga acgaaaccaa
    10141 agataaattg catcagagcg gttactctct ctctggtggt cagcaacagc gtctgtgcat
    10201 tgcgcgtggt atcgccattc gcccggaagt gctgctgctc gacgaaccat gttcggcgct
    10261 cgaccccatc tccaccgggc gtattgaaga gctgatcacc gaactgaagc aggattacac
    10321 cgtggtgatc gtcacccaca acatgcagca ggctgcgcgt tgttccgacc acacggcgtt
    10381 tatgtacctg ggcgaattga ttgagttcag caacacggac gatctgttca ccaagccagc
    10441 gaagaaacaa acagaagact acatcaccgg tcgttacggt tgattcagga gtgcgttatg
    10501 gacagtctca atcttaataa acatatttcc ggccagttca acgccgaact ggaaagtatc
    10561 cgcacgcagg tgatgaccat gggcggcatg gtggagcagc agctttctga tgcaatcacc
    10621 gcgatgcata accaggacag cgatctggcg aagcgcgtga tcgaaggcga caagaacgtc
    10681 aacatgatgg aagtggcgat cgatgaagcc tgcgtgcgca ttattgccaa acgccagccg
    10741 acggcgagcg acctgcgatt ggttatggtg atcagtaaaa ccattgccga actggagcgt
    10801 attggcgacg tggcggacaa aatctgccgt actgcgctgg agaaattctc ccagcagcat
    10861 cagccgttgc tggtaagttt ggagtcgctg ggccgtcata ctatccagat gctgcacgac
    10921 gtgctggacg cgttcgcgcg gatggacatt gacgaagcgg tacgtattta tcgtgaagat
    10981 aaaaaagtcg atcaggagta cgaaggtatt gttcgtcaac tgatgaccta catgatggaa
    11041 gattcgcgca ccattccgag tgtacttacc gcgctgttct gtgcgcgttc tatcgaacgt
    11101 attggcgacc gctgccagaa tatttgtgag tttatcttct actacgtgaa gggtcaggat
    11161 ttccgtcatg taggcggtga tgagctggat aaactgctgg cgggaaaaga ccccaaagag
    11221 tagtgctaac tgtaggccag ataaagcttt ttgccgcatc tggcaatcag cccggtcata
    11281 catagttacc gggctgatac actttctcac tgaaatattg ccacacaaac tcacctcgaa
    11341 actctcccca gtacattgaa tatacattat ttcatttgaa ttattttact tttaatgtag
    11401 tgcctcgcta taaaaacata tttgttattc ttttcattta tatcgctacc tgcacagaga
    11461 atggaaaaga aaacaaaaaa cagggtaaaa gccctatatc gagacataaa aaatgagcga
    11521 ttttttacag tattcatagg gggtttagtc ataatcgtag caaaccaaca cccaggtatg
    11581 ctttttaaga caaatcggca atgtattatg ctcgggcgaa taaatattgt g
//
LOCUS       DAZKIF010000130        11620 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZKIF010000130 DAZKIF010000000
VERSION     DAZKIF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.131"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..189
                     /locus_tag="ACHOTW_003818"
     CDS             <1..189
                     /locus_tag="ACHOTW_003818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845842.1"
                     /translation="HWNGFSGEKRILYGHSAHHFFFSSSTIHVSLPRLITDYEVQKYL
                     SFQLPVIRYRERFWQYME"
     gene            196..2619
                     /gene="pgaA"
                     /locus_tag="ACHOTW_003819"
     CDS             196..2619
                     /gene="pgaA"
                     /locus_tag="ACHOTW_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFP9845843.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2628..4646
                     /gene="pgaB"
                     /locus_tag="ACHOTW_003820"
     CDS             2628..4646
                     /gene="pgaB"
                     /locus_tag="ACHOTW_003820"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFP9845844.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4639..5964
                     /gene="pgaC"
                     /locus_tag="ACHOTW_003821"
     CDS             4639..5964
                     /gene="pgaC"
                     /locus_tag="ACHOTW_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFP9845845.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5966..6379
                     /gene="pgaD"
                     /locus_tag="ACHOTW_003822"
     CDS             5966..6379
                     /gene="pgaD"
                     /locus_tag="ACHOTW_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFP9845846.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6429..7217)
                     /gene="phoH"
                     /locus_tag="ACHOTW_003823"
     CDS             complement(6429..7217)
                     /gene="phoH"
                     /locus_tag="ACHOTW_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP9845847.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7837..9108)
                     /gene="efeB"
                     /locus_tag="ACHOTW_003824"
     CDS             complement(7837..9108)
                     /gene="efeB"
                     /locus_tag="ACHOTW_003824"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP9845848.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9114..10241)
                     /gene="efeO"
                     /locus_tag="ACHOTW_003825"
     CDS             complement(9114..10241)
                     /gene="efeO"
                     /locus_tag="ACHOTW_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP9845849.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10299..11129)
                     /gene="efeU"
                     /locus_tag="ACHOTW_003826"
     CDS             complement(10299..11129)
                     /gene="efeU"
                     /locus_tag="ACHOTW_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP9845850.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3061 a         2848 c         2721 g         2990 t
ORIGIN      
        1 cattggaatg gattttcggg cgagaaaagg attttatatg gacactctgc tcatcatttc
       61 ttcttctcat catcaacaat tcacgtctct cttccgcgtt taataacgga ttatgaggtg
      121 caaaaatatc tttcttttca gttacctgta attagataca gagagagatt ttggcaatac
      181 atggagtaat acaggatgta ttcaagtagc agaaaaaggt gcccgaaaac caaatgggct
      241 ttgaaacttc ttactgccgc atttttagca gcgagtcccg cggcgaagag tgctgttaat
      301 aacgcctatg atgcattgat tattgaagct cgcaagggta atactcagcc agctttgtca
      361 tggtttgcac taaaatcagc actcagcaat aaccaaattg ctgactggtt acagattgcc
      421 ttatgggccg ggcaagataa acaggttatt accgtttaca accgctaccg tcatcagcaa
      481 ttaccagcgc gtggttatgc agctgtcgcc gtcgcttatc gtaacctgca acaatggcaa
      541 aactcgctta cactgtggca aaaggcgctc tctctggagc cgcaaaataa ggattatcaa
      601 cggggacaaa ttttaaccct ggcagatgct ggtcactatg atactgcgct ggttaaactt
      661 aagcagctta actctggagc accggacaaa gccaatttac tcgcagaagc ctatatctat
      721 aaactggcgg ggcgtcatca ggatgaatta cgggcgatga cagagtcatt acctgaaaat
      781 gcatctacgc aacaatatcc cacagaatac gtgcaggcat tacgtaataa tcaacttgct
      841 gccgcgattg acgatgccaa tttaacgcca gatattcgcg ctgatattca tgccgaactg
      901 gtcagactgt cgtttatgcc tacgcgcagt gaaagtgaac gttatgccat tgccgatcgc
      961 gccctcgccc aatacgctgc attagaaatt ctgtggcacg ataacccaga ccgtactgcc
     1021 cagtaccagc gtattcaggt tgatcatctt ggcgcgttat taactcgcga tcgttataaa
     1081 gacgttattt ctcactatca gcgattaaaa aagacggggc aaattattcc gccctggggg
     1141 caatattggg ttgcatcggc ttatctcaaa gatcatcagc cgaaaaaagc acagtcaata
     1201 atgaccgagc tcttttatca caaggagacc attgccccgg atttatccga tgaagaactt
     1261 gcggatctct tttacagcca cctggagagt gaaaattatc cgggcgcgct aactgtcacc
     1321 caacatacca ttaatacttc gccgcctttc cttcggttaa tgggcacgcc tacgagcatc
     1381 ccgaatgata cctggttaca ggggcattcg tttctctcaa ccgtagcaaa atatagtaat
     1441 gatcttcctc aggctgaaat gacagccaga gagcttgctt ataacgcacc aggaaatcag
     1501 ggactgcgca ttgattacgc gagtgtgtta caagcccgcg gttggcctcg tgcagcagaa
     1561 aatgaattaa aaaaagcaga agtgatcgag ccacgtaata ttaatctgga ggttgaacaa
     1621 gcctggacag cattaacgtt acaagaatgg cagcaggcag ctgtcttaac gcacgatgtt
     1681 gtcgaacgtg aaccgcaaga tcccggcgtt gtacgattaa aacgtgcggt tgatgtacat
     1741 aatcttgcag agcttcgtat cgctggctca acaggaattg atgccgaagg cccggatagt
     1801 ggtaaacatg atgtcgactt aaccaccatc gtttattcac caccgctgaa ggataactgg
     1861 cgcggttttg ctggattcgg ttatgccgat ggacaattta gcgaaggaaa agggattgtt
     1921 cgcgactggc ttgcgggtgt tgagtggcgg tcacgtaata tctggctcga ggcagagtac
     1981 gctgaacgcg ttttcaatca tgagcataaa cccggcgcgc gcctgtctgg ctggtatgat
     2041 tttaatgata actggcgtat tggttcgcaa ctggaacgcc tctctcaccg cgttccatta
     2101 cgggcaatga aaaatggtgt tacaggcaac agtgctcagg cttatgttcg ctggtatcaa
     2161 aatgagcggc gtaagtacgg tgtctcctgg gctttcactg atttttccga cagtaaccag
     2221 cgtcatgaag tctcacttga gggtcaggaa cgcatctggt cttcaccata tttgattgtc
     2281 gatttcctac ccagtctgta ttacgaacaa aatacagaac acgatacccc atactacaac
     2341 cctataaaaa cgttcgatat tgttccggca tttgaggcaa gccatttgtt atggcgaagc
     2401 tatgaaaata gctgggagca aatattcagc gcaggtgttg gtgcctcctg gcaaaaacat
     2461 tatggcacgg atgtcgtcac ccaactcggc tacgggcaac gcattagctg gaatgacgtg
     2521 attgatgctg gcgcaacgct acgctgggaa aaacgacctt atgacggtga cagagaacac
     2581 aacttatacg ttgaattcga tatgacattc agattttaag gataaatatg ttacgtaatg
     2641 gaaataaata tctcctgatg ctggtgagta taattatgct caccgcgtgc attagccagt
     2701 caagaacatc atttataccg ccacaggatc gcaaatcttt actcgccgag caaccgtggc
     2761 cgcataatgg ttttgtagcg atttcatggc ataacgttga agacgaagct gccgaccagc
     2821 gttttatgtc agtgcggaca tcagcactgc gtgaacaatt tgcctggctg cgcgagaacg
     2881 gttatcaacc ggtcagtatt gctcaaattc gtgaagcaca tcgaggagga aaaccgctac
     2941 cggaaaaagc tgtagtgctg acttttgatg acggctacca gagtttttat acccgcgtct
     3001 tcccaattct tcaggccttc cagtggcctg ctgtatgggc ccccgtcggc agttgggtcg
     3061 atacgccagc ggataaacaa gtaaaatttg gcgatgagtt ggtcgatcga gaatattttg
     3121 ccacgtggca acaagtgcga gaagttgcgc gttcccggct cgttgagctc gcttctcata
     3181 catggaattc tcactacggt attcaggcta atgccaccgg cagcttattg cctgtatatg
     3241 taaatcgtgc atattttact gaccacgcac ggtatgaaac cgcagcagaa taccgggaaa
     3301 gaattcgtct ggatgctgta aaaatgacgg aatacctgcg tacaaaggtt gaggtaaatc
     3361 cacacgtttt tatttggcct tatggcgaag cgaatggcat agcgatagag gaattaaaaa
     3421 aactcggtta tgacatgttc ttcacccttg aatcaggttt ggcaaatgcg tcgcaattgg
     3481 attccattcc gcgggtatta atcgccaata atccctcatt aaaagagttt gcccagcaaa
     3541 ttattaccgt acaggaaaaa tcaccacaac ggataatgca tatcgatctt gattacgttt
     3601 atgacgaaaa cctccagcaa atggatcgca atattgatgt gctaattcag cgggtgaaag
     3661 atatgcaaat atcaaccgtg tatttgcagg catttgctga tcccgatggt gatgggctgg
     3721 tcaaagaggt ctggtttcca aatcgtttgc taccaatgaa agcagatatt tttagtcggg
     3781 ttgcctggca attacgtacc cgctcaggtg taaacatcta tgcgtggatg ccggtattaa
     3841 gctgggattt agatcccaca ttaacgcgag taaaatactt accaacaggg gagaaaaaag
     3901 cacaaattca tcctgaacaa tatcaccgtc tctctccttt cgatgacaga gtcagagcac
     3961 aagttggcat gttatatgaa gatcttgccg gacatgctgc ttttgatggc atattgttcc
     4021 acgatgatgc tttgctttca gattatgaag atgccagtgc accggctatc acggcttatc
     4081 agcaagcagg ctttagcggg agtctgagcg aaattcgaca aaacccggag caatttaaac
     4141 agtgggcccg ctttaaaagt cgtgcgttaa ctgacttcac tttagaactt agtgcgcgcg
     4201 taaaagccat tcgcggtcca catattaaaa ctgcacgaaa tatttttgca cttccggtaa
     4261 tacaacctga aagtgaagcc tggtttgcac agaattatgc tgatttccta aaaagctatg
     4321 actggaccgc tattatggct atgccttatc tggaaggtgt cgcagaaaaa tcggctgacc
     4381 aatggttaat acaattgacc aatcaaatta aaaacatccc tcaggctaaa gacaaatcta
     4441 ttttagaatt acaggcacaa aactggcaga aaaatggtca gcatcaggct atttcttcgc
     4501 aacaactcgc tcactggatg agcctattac aactgaatgg agtgaaaaac tatggttatt
     4561 atcccgacaa ttttctgcat aaccaacctg aaatagacct tattcgtcct gagttttcaa
     4621 cagcctggta tccgaaaaat gattaatcgc atcgtatcgt tttttatatt atgtctggtg
     4681 ttatgcatac ccctatgcgt agcgtacttt cactctggtg aactgatgat gaggttcgtt
     4741 ttcttctggc cgttttttat gtccattatg tggattgttg gcggcgtcta tttctgggtc
     4801 tatcgtgaac gccgctggcc gtggggagaa aacgcaccag ctccccagtt gaaagataat
     4861 ccgtctatct ccattatcat tccctgtttt aatgaggaga aaaacgttga ggaaaccata
     4921 cacgccgctt tagcacagcg ttatgagaac attgaagtta ttgccgtaaa tgacggttca
     4981 acagataaaa cccgtgccat cctggatcgc atggctgcac aaattcccca tttgcgggtc
     5041 attcatctgg cgcaaaacca ggggaaagcc attgcgctta aaaccggagc tgccgcggcg
     5101 aaaagtgaat atctggtgtg cattgatggc gatgcgttat tagaccgcga tgcggcggca
     5161 tatattgtgg aaccgatgtt gtacaacccg cgtgtgggtg ccgtaaccgg taatcctcgt
     5221 attcgaacac gttctaccct ggtgggtaaa attcaggttg gcgagtattc ctcaattatt
     5281 ggtttgatca agcgaaccca gcgtatctat ggaaacgtat ttaccgtttc cggtgttatt
     5341 gccgcatttc gtcgcagcgc cctggcagaa gtgggttact ggagtgacga tatgatcacc
     5401 gaagatattg atattagctg gaagctgcag ttgaatcagt ggacgatttt ttacgagcca
     5461 cgggcactgt gctggatatt aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg
     5521 cgctgggctc agggcggtgc agaagtattc ctcaaaaata tgacaaggtt gtggcgcaaa
     5581 gaaaactttc gaatgtggcc gctgtttttt gaatacagcc tgacgacaat atgggccttc
     5641 acctgcctgg tcggtttcat tatttacgca gtccaacttg ccggtgtacc gttaaatatt
     5701 gaattgacac atatcgctgc gacacatact gccggaatat tattgtgtac gttatgttta
     5761 ctgcaattta ttgtcagcct gatgatcgag aatcgctatg agcataatct gacttcatcg
     5821 cttttctgga ttatttggtt cccggttatt ttctggatgc tgagcctggc aacgacattg
     5881 gtatcattta cacgagtcat gttgatgcct aaaaagcaac gcgcccgttg ggtaagtccc
     5941 gatcgcggga ttctgagagg ttaatatgaa caatttaatt attacgaccc gacaatcacc
     6001 agtacgttta ctggttgatt atgttgccac aaccatcttg tggacattat ttgcgttgtt
     6061 catattctta ttcgccatgg atctgctgac gggttattac tggcaaagcg aggccagaag
     6121 ccgacttcag ttctattttt tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc
     6181 gctgtacaat aagctgcgtt ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc
     6241 gcaagaatat gcagagagct tagcaatacc tgatgagctc tatcagcaac tacaaaaaag
     6301 ccacaggatg agcgtacact tcaccagcca ggggcaaata aaaatggttg tttcagaaaa
     6361 agcgctagtc agggcataaa cacccaaaac aaagcccggt tagcccgggc tctgcaccga
     6421 taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc
     6481 tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca
     6541 cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata
     6601 accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc agtcacattc
     6661 tgcgcctcgt caagaatgac gactgcattt tcaaaggtac gtccacgcat ataggcgaac
     6721 ggcgcaattt ccaccttccc tatttccggt cgcaggcagt actgcataaa ggaagcccct
     6781 aagcgccgga ccagcacgtc gtagaccggg cgaaaatagg gagcaaactt ttctgcgata
     6841 tctccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt gacgataatc
     6901 ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat ccaggttttt
     6961 ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc cttcagatag
     7021 tgcaattgcg cttcatttcg cgcgaggatg ggcgaagtat cgcgactgtc gcgggccata
     7081 ccaatggctt ctacgccgcc catctgcaca agcgaggtga ccgattcttc ttcacgctgc
     7141 ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc tttgatcact
     7201 gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca cgcgccgttg
     7261 gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc atagtttgct
     7321 tttggataaa atgccgaaaa acggctacgc gcaccgttta cggcgtcggt aacacatgaa
     7381 aagaaaggat gaggttgaaa atgcaaagtg acgagatgac taccggagga gaaaactccg
     7441 cgagtggtgg cgcgttgatt atctaaaaca tgtccagtac aggacgttac catccgcgat
     7501 ctccatagtg actgactatc actgccggga acttccgctg ctacttaata agtacaacag
     7561 ttctcgcatt tattgcaaca atatatttac ttatatttaa ctataaaaca ccatttcagt
     7621 gacattagtt tctactggaa agatgacaga gtgatgacag atatgaaaaa agctgtgtgc
     7681 tttcagcagg atttgcatca ggcatcagtg cacgatcgcc gtatgcggcg tgaacgcctt
     7741 atccgcctac cgaacccgca ccgattgtag gcataagaag acgcgcaagc atcgcatcag
     7801 gcatctgcgc aagaacgcct tatccgtcta aaaacattaa acccgcaata acgcgcttcc
     7861 gagataatcg ttcgcgtcct tcactcccgg cagcgcaaaa aaataaccgc cgccgatagg
     7921 tttaacgtat tcctccagcg cttcgccatt gagccttttt tgtactgtca ggaagccttt
     7981 ttccagatcg tgttggtaac agacaaacag tagccccata tccagttgcc cggagttggt
     8041 gacgcccagt gaataactgt agccacgacg cagcatcagg ctggactcac tctccgccgt
     8101 gcggggattc gccagccgga tatggctgtc cagcgcaatc cccttccctt ccgggtcgct
     8161 ggcgtaatca ggcacatcat gctcatgctg cattcccagc ggcgcaccgg tttgcttatc
     8221 acggccaaaa atcgtctgct gttctttcag cggcgttctg tcccaaaatt ccactcgaaa
     8281 ctgaatcaag cgtactgcct gatagctgcc accgattgtc cacgcaggct cctgctgatc
     8341 tgccgttacc cacaccactt tttgcatcaa cttatcattc tggctatcgg gattggcagt
     8401 gccgtctttg aaacccagca aattaatcgg cgtctctttg cctttactac gcgccgcgtg
     8461 atcggaaata aacccttccc gcttccagcg cacgctgagc aaatccggcg tatgtttgat
     8521 gatatcgcgc agtgcatgga tcaccgtgtc ctgggtgttg gcgcaaattt gtagcaacac
     8581 atcaccatga cataacgccg catccagcga gtcgttggga aaacgcgtca tcttctgcaa
     8641 ctttttcggc atctgtggcg caaggccaaa gcgctcatca aacaatgagt gacccaccga
     8701 taacgtgatg gtgagattat cgggcgcaat gtagccgcca agaatgccgg aatcgagtgg
     8761 tggcaggcgc ggatttggcg tttctggtgc cgctccgccc tgagtcagaa aagcaaaacg
     8821 ctgagtcaac aagcgaaaca accgttcaag atcggcttta tcgctggcaa gcacatcaaa
     8881 cgccaccagc atcattgcgg cctgttgtgg cgtcaggatc cctgcctgat gctcaccata
     8941 aaacggctgt ttctcattgc gtgcaaccgg tgaaagcgta cccggcgcac tttgcgtttt
     9001 ttgtgcatga gcgaccggac aacttcccgc cagcgccagc gcaccaatgc ctttcagtaa
     9061 acgtcggcgt gacggttcat tcacgccgtt tttatcttca tactgcataa tgcttaatcc
     9121 aatcccagca caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggtcctttc
     9181 agtgcattcc ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg
     9241 tatttcgcca gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc
     9301 gggttagctt tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg
     9361 ttagcctgga aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg
     9421 ctggctgcca cttcctcaat cagtccggct gcaccgccga ccacttttga aggtgggaaa
     9481 gccagttcac tgatgcgttt ttgcaaatcg accacatcgg tataaagctg gtcagcgtac
     9541 tgatccatgc ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaaccg
     9601 gtgaattttg gatcagcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca
     9661 tccagatcgg agaacagttc agcaatcggt tcaatgcgct catagtgctg gcgcgtcggc
     9721 gcatacagtg ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg
     9781 gtgtcggtca ccagctgtgt ggtttccgcc atgacatacg ctttatatgc agtaattgca
     9841 ccaccaagac ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg
     9901 atcaacttcc ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt
     9961 aaattcgccg tcattttctg gctaaagcca ggggcgatat tttcccgctc ttccaccacc
    10021 atcacgcctt tgaggatctc ccattccagc gccttctggc tgtggttctg aataatgaac
    10081 tgtgttttcc cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc
    10141 actttgacct gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc
    10201 acgctcaact gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa
    10261 agtatgttgt aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc
    10321 gcccctgcgc gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag
    10381 acggcgactt cgctgacgct cggcgcttcc tgatagccaa aaatcccttc catcagcgtg
    10441 ccaaacagcg agtgagttga gagcaccgca ctcatatcga aggcgatttc ctgaaagtgg
    10501 ttccacaatc cggcttcatg aaatgcgcga atggcaccag ctgccagccc tgcggcgacg
    10561 aagagaataa acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc
    10621 cagtagagca ggaagcctag caccacggca gtagcaagac cgagcattgc acccagcggc
    10681 ggccagatcc cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct
    10741 tcccttgcaa cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc
    10801 tgcaatgcgc tatcgacagc ctgctccagt tgtactttga cgttgcgcga cactttgcgc
    10861 atccagaaaa ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac
    10921 agttcctgct ctttttgcgg aaattcgccg gtggtttcgt taatgaagat ccccaagccc
    10981 aggcacaacg cagcggcaag caacacgcca atccacatca caccaatcca tcggcctcgc
    11041 tgggtacgct taagatagct ggcaatcaaa ctgacaatca gcgcggcttc aagtccttcg
    11101 cgcaacataa tgagaaacgg aacaaacatg ccacccaccc ttaaacgttt ttctgagtca
    11161 atctatgcaa agaaaagtaa atcgcagtga tagcgattat cattacgcta aagaaaaata
    11221 caagcggaat atttataaaa tgatgttgga atgtaaaaat gaggagatca aatccggaga
    11281 aaaaagcgat tattttaagg tatttagctg acgtttcgcc cggcagatga agcttgtacg
    11341 aatgttggtg gcgacaaact cgctgcagcg tgcccgaagg gttgaatttt cagttagtcg
    11401 tttcacaaaa gaacgttgtt ttttgcttat taatagatag tgataaggtt gtgaatgaaa
    11461 ttatacctca tctcctcatc gaattttttg cccacaacct aaacaggaga aaacgtgtat
    11521 ttaagtctgg aatgagcgat ttttgatagt gcctaattat cccccaaaat tataattccc
    11581 cttattatat tcccagtgat atcattttat ggctaatgct
//
LOCUS       DAZKIF010000131        11554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZKIF010000131 DAZKIF010000000
VERSION     DAZKIF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.132"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(107..1189)
                     /gene="lptG"
                     /locus_tag="ACHOTW_003827"
     CDS             complement(107..1189)
                     /gene="lptG"
                     /locus_tag="ACHOTW_003827"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP9845851.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(1189..2289)
                     /gene="lptF"
                     /locus_tag="ACHOTW_003828"
     CDS             complement(1189..2289)
                     /gene="lptF"
                     /locus_tag="ACHOTW_003828"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP9845852.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(2352..2402)
                     /gene="ytgA"
                     /locus_tag="ACHOTW_003829"
     CDS             complement(2352..2402)
                     /gene="ytgA"
                     /locus_tag="ACHOTW_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP9845853.1"
                     /translation="MSIIILATISTTQENK"
     gene            2556..4067
                     /gene="pepA"
                     /locus_tag="ACHOTW_003830"
     CDS             2556..4067
                     /gene="pepA"
                     /locus_tag="ACHOTW_003830"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP9845854.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            4421..4864
                     /gene="holC"
                     /locus_tag="ACHOTW_003831"
     CDS             4421..4864
                     /gene="holC"
                     /locus_tag="ACHOTW_003831"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP9845855.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            4864..7719
                     /gene="valS"
                     /locus_tag="ACHOTW_003832"
     CDS             4864..7719
                     /gene="valS"
                     /locus_tag="ACHOTW_003832"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9845856.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(7774..8970)
                     /gene="yjgN"
                     /locus_tag="ACHOTW_003833"
     CDS             complement(7774..8970)
                     /gene="yjgN"
                     /locus_tag="ACHOTW_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFP9845857.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGVWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIVSVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            9163..9666
                     /gene="yjgM"
                     /locus_tag="ACHOTW_003834"
     CDS             9163..9666
                     /gene="yjgM"
                     /locus_tag="ACHOTW_003834"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9845858.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(9712..10128)
                     /gene="rraB"
                     /locus_tag="ACHOTW_003835"
     CDS             complement(9712..10128)
                     /gene="rraB"
                     /locus_tag="ACHOTW_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP9845859.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            10290..11294
                     /gene="argF"
                     /locus_tag="ACHOTW_003836"
     CDS             10290..11294
                     /gene="argF"
                     /locus_tag="ACHOTW_003836"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9845860.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(11351..>11554)
                     /locus_tag="ACHOTW_003837"
     CDS             complement(11351..>11554)
                     /locus_tag="ACHOTW_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845861.1"
                     /translation="KNQPKDVIEEKLNNELKLRFRMMQTILKSEVNVSPFIDQQRLNT
                     LNPPENLRIAIEKFGWKKKTITA"
BASE COUNT         3052 a         2948 c         2907 g         2647 t
ORIGIN      
        1 accccactgc aatatattga attttaatta tttttcagga atgaacgaag cacactttgc
       61 taacaataat aaagggagct ttcgctccct ttattcgttc attcggttac gattttctca
      121 ttaacagcca caggctgatt aagaagaagc tggcgcttgg caacagtgcg ccgatgatcg
      181 gcgggatgcc ataaaccaac gtcagcgggc cgaagatctg gtccagtacg tagaagacaa
      241 aaccgaaact gataccggtg accacacgca cgcccatcgg tacgctacgc agtgggccaa
      301 agatgaacga cagcgccatc agcatcatca ccgccacgga tagcggctgg aagattttgc
      361 tccacatgtt gagctgataa cgtccggcat cctgaccgct cgacttcaga tacttcacat
      421 agttgtgcaa accgctgata gagagtgcat ccgggtccag cgccaccacg cccagtttgt
      481 ccggcgtgag gttggttttc caggtgccgc tcaccgtctg cgaaccggta atctgtttcg
      541 gattggtcag atcagattca tcaacctgcg acaaacgcca gactttatgt tccgggtcaa
      601 acttcgcagt agcggcatag cgtacggatt gcagacgacg attctcgtta aaggcataaa
      661 tgctgatgcc acctaactct tcgtcacctt taacccgctc aatgtagacg aagttgttgc
      721 catctttcgc ccataagcct tgctgggtag agagcaacga gccgccgtac atcgcctgcg
      781 cacggtagtt acgcgccatc tgctcgccct gcggcgcgac ccattcgcca atcgccatcg
      841 tcagcaagac cagcggaatg gcggttttca tcaccgacag cgccacctgc atacgggtaa
      901 aaccagaagc ctgcatcacc accagttcgc tgcgctgcgc cagcatccca agaccaagca
      961 acgccccaag cagagccgcc atcgggaaga atatttgcac atctttcggc acgctcagca
     1021 aggtatacat tcctgcgcct aacgcgtcgt aactcccctg cccggctttt ttcaactgat
     1081 cgacaaactt gataatgccc gacagcgaca ccagcatgaa cagcgtcatc atgatggtgg
     1141 tgaaaatagt tttaccgata tagcggtcaa gtacgccaaa aggttgcatc acaccgctcc
     1201 tttacgcgaa aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa ggttgagaac
     1261 aatcgccaaa gccagataaa tcaggttaac ggtccacatc cacagcgtcg ggtccagctt
     1321 acctttaccg ccgttcgatt tcagggaggt ctggatcagg aagaaaagta gatacagcag
     1381 catggctggc agcatcgaca gtacgcgtcc ctgacgtggg ttaaccacgc tcagcggtac
     1441 gaccataagt gccatcataa acacggtgaa taccaacgtg atacgccagt tcagttctgc
     1501 gcgagcacga tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt cggtatcgtt
     1561 cgggtcgagc gccaccgcct ggtgaccaat gatcgcctga taatcctgga aatccgtaat
     1621 gcggaaatca cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga gagtgacgac
     1681 ctgggagccg tcgcgcagct gggttaaatg tccggaatcg gccaccacca cagaaggacg
     1741 tgcattacct tttggtcgaa tttgcgcgag gaacacatct ttgaaatcgc tgccgtcaac
     1801 gctttcgatg aacagcaccg aactgccatt agtcgcttgc tggaattgcc cttgcgccag
     1861 cgccgccatg ccagggttcg ctttcgcttc tgctaacact tcatcctgat gacgcgatga
     1921 ccacggtccc gcccacatca cgttaaccgc cgcgacgatt gccgtgaata ccgcaaggat
     1981 cattgccgct ttcaccagaa ccgctttgct caggccgcag gcatgcatta ccgtaatttc
     2041 actttcggta tacagtttgc ccagcgtcat cagcagcccg aggaacaggc ttaatggcag
     2101 gataagctgc gccatttccg gcacgcccaa cccgagaagg gagagcacca gattcgccgg
     2161 aatatcgccg tcaaccgctg cgccgaggat cctcactaac ttttgacaga agaagatcaa
     2221 aagcaagatg aagagtatcg ccagctggct tttgagcgtc tcccgcacca gatatcttat
     2281 gattatcact ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta gcttgtttca
     2341 tggcttaaac gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat aattatactc
     2401 aacggattca cctctcagat tttgttctga cgtgccaatg ccgtaataac gttaagatta
     2461 acacgaagtc atcgcaacag cggacatgag ttacgaaagc ttgcaattct atctgtagcc
     2521 accgccgttg tctttaagat tcaggagcgt agtgcatgga gtttagtgta aaaagcggta
     2581 gcccggagaa acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca cgtcgccttt
     2641 ctccgattgc agaacagctc gataaaatca gcgatgggta catcagcgcc ctgctacgtc
     2701 ggggcgaact ggaaggaaaa ccggggcaga cattgttgct gcaccatgtt ccgaatgtac
     2761 tttccgagcg aattctcctt attggttgcg gcaaagaacg tgagctggat gagcgtcagt
     2821 acaagcaggt tattcagaaa accattaata cgctgaatga tactggctca atggaagcgg
     2881 tctgctttct gactgaactg cacgttaaag gccgtaacaa ctactggaaa gtgcgtcagg
     2941 ctgtcgagac ggcaaaagag acgctctaca gtttcgatca gctgaaaacg aacaagagcg
     3001 aaccgcgtcg tccgctgcgt aagatggtgt tcaacgtgcc gacccgccgt gaactgacca
     3061 gcggtgagcg cgcgatccag cacggtctgg cgattgccgc cgggattaaa gccgcgaaag
     3121 atctcggtaa tatgccgccg aatatctgta acgccgctta cctcgcttca caagcgcgcc
     3181 agctggctga cagctacagc aagaatgtta tcacccgcgt tatcggcgaa cagcagatga
     3241 aagagctggg gatgcattcc tatctggcgg tcggtcaggg ttcgcaaaac gaatcgctga
     3301 tgtcggtgat tgagtacaaa ggcaacgcgt cggaagatgc acgcccaatc gtgctggtgg
     3361 gtaaaggttt aaccttcgac tccggcggta tctcgatcaa gccttcagaa ggcatggatg
     3421 agatgaagta cgatatgtgc ggtgcggcag cggtttacgg cgtgatgcgg atggtcgcgg
     3481 agctacaact gccgattaac gttatcggcg tgttggcagg ctgcgaaaac atgcctggcg
     3541 gacgagccta tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc gttgaagtgc
     3601 tgaataccga cgctgaaggc cgcctggtac tgtgcgacgt gttaacttac gttgagcgtt
     3661 ttgagccgga agcggtgatt gacgtggcga cgctgaccgg tgcctgcgtg atcgcgctgg
     3721 gtcatcacat taccggtctg atggcgaacc ataatccgct ggcccatgaa ctgattgccg
     3781 cgtctgaaca atccggtgac cgcgcatggc gcttaccgct gggtgacgag tatcaggaac
     3841 aactggagtc caattttgcc gatatggcga acattggcgg tcgtcctggt ggggcgatta
     3901 ccgcaggttg cttcctgtca cgctttaccc gtaagtacaa ctgggcgcac ctggatattg
     3961 caggaaccgc ctggcgttct ggtaaagcaa aaggcgcaac cggtcgtccg gtagcgttgc
     4021 tggcacagtt cctgttgaac cgcgctgggt ttaacggcga agagtaattg cgtcaggcaa
     4081 ggctgttatt gccggatgcg gcgtgaacgc cttatccgac ctacacagca ctgaactcgt
     4141 aggcctgata agacgcgcaa gcgtcgcatc aggcaatgct gttattgtcg gatgcggcat
     4201 gaacgcctta tccgacctac acagcaccga actcgtaggc ctgataagac gcggcagcgt
     4261 cgcatcaggc aatgctgtta ttgtcggatg cggcgtgagc gccttatccg acctacacac
     4321 agcactgaac tcgtaggcct gataagacac aacagcgtcg catcaggcgc tgcggtgtat
     4381 acctgatgcg tatttaaatc caccacaaga agccccatat atgaaaaacg cgacgttcta
     4441 ccttctggac aatgacacca ccgtcgatgg cttaagcgcc gttgagcaac tggtgtgtga
     4501 aattgccgca gaacgttggc gcagcggtaa gcgcgtgctc atcgcctgtg aagatgaaaa
     4561 gcaggcttac cggctggatg aagccctgtg ggcgcgtccg gcagaaagct ttgttccgca
     4621 taatttagcg ggagaaggac cgcgcggcgg tgcgccggtg gagatcgcct ggccgcaaaa
     4681 acgcagcagc agcccgcgcg atatattgat tagtctgcga acaagctttg cagattttgc
     4741 caccgctttc acagaagtgg tagacttcgt tccttatgaa gattctctga aacaactggc
     4801 gcgcgaacgc tataaagcct accgcgtggc tggtttcaac ctgaatacgg caacctggaa
     4861 ataatggaaa agacatataa cccacaagat atcgaacagc cgctttacga gcactgggaa
     4921 aagcagggct actttaagcc taatggcgat gaaagccagg aaagtttctg catcatgatc
     4981 ccgccgccga acgtcaccgg cagtttgcat atgggtcacg ccttccagca aaccatcatg
     5041 gataccatga tccgctatca gcgcatgcag ggcaaaaaca ccctgtggca ggtcggtact
     5101 gaccacgccg ggatcgctac ccagatggtc gttgagcgca agattgccgc agaagaaggt
     5161 aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca aaatctggga atggaaagcg
     5221 gaatccggcg gcacgattac ccgtcagatg cgccgtctcg gcaactccgt cgactgggaa
     5281 cgtgaacgct tcacgatgga cgaaggcctg tccaatgcgg tgaaagaagt tttcgttcgt
     5341 ctatataaag aagacctgat ttaccgtggc aaacgcctgg taaactggga cccgaaactg
     5401 cgcaccgcta tctctgacct ggaagtggaa aaccgcgaat cgaaaggttc gatgtggcac
     5461 atccgctatc cgctggctga cggtgcgaaa accgcagacg gtaaagatta tctggtggtc
     5521 gcgactaccc gtccggaaac cctgctgggc gatactggcg tagccgttaa cccggaagat
     5581 ccgcgttaca aagatctgat tggcaaatat gtcattctgc cgctggttaa ccgtcgtatt
     5641 ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca ccggctgcgt gaaaatcact
     5701 ccggcgcacg actttaacga ctatgaagtg ggtaaacgtc acgcgctgcc gatgatcaac
     5761 atcctgacct ttgacggcga tatccgtgaa agcgcccagg tgttcgatac caaaggtaac
     5821 gaatctgacg tttattccag cgaaatcccg gcagagttcc agaaactcga gcgttttgct
     5881 gcacgtaaag cggtcgttgc tgcggttgac gcactcggcc tgctggaaga aattaaaccg
     5941 cacgacctga cagttcctta cggcgaccgt ggcggcgtag ttatcgaacc aatgctgacc
     6001 gaccagtggt acgtacgtgc cgatgtactg gcgaagccgg cagttgaagc ggttgagaac
     6061 ggcgacattc agttcgtacc gaagcagtac gaaaacatgt acttctcctg gatgcgcgat
     6121 attcaggact ggtgtatctc tcgtcagctg tggtggggtc accgtatccc ggcatggtac
     6181 gacgaagcgg gtaacgttta tgttggccgc aacgaagaag aagtgcgcaa ggaaaataac
     6241 ctcggtgccg acgttgccct gcgtcaggac gaagacgttc tcgacacctg gttctcttct
     6301 gcgctgtgga ccttctctac cctaggctgg ccggaaaaca ccgacgcact gcgccagttc
     6361 cacccaacca gcgtgatggt atctggtttc gacatcatct tcttctggat tgcccgcatg
     6421 atcatgatga ccatgcactt catcaaagat gaaaatggca aaccgcaggt gccgttccac
     6481 accgtttaca tgaccggtct gattcgtgac gacgaaggcc agaagatgtc caaatccaag
     6541 ggtaacgtta tcgacccgct ggatatggtt gacggtattt cgctgccaga actgctggaa
     6601 aaacgtaccg gcaatatgat gcagccgcag ctggcggaca aaatccgtaa gcgcaccgag
     6661 aagcagttcc cgaacggtat tgagccgcac ggcaccgatg ccctgcgctt caccctggcg
     6721 gcactggcgt ctaccggtcg tgacatcaac tgggatatga agcgtctgga aggctaccgt
     6781 aacttctgta acaagctgtg gaacgccagc cgctttgtgc tgatgaacac ggaaggtcag
     6841 gattgcggct tcaacggcgg cgaaatgacg ctgtcgctgg cggaccgctg gattctggcg
     6901 gagttcaacc agaccatcaa agcgtaccgc gaagcgctgg acagcttccg cttcgatatc
     6961 gccgcaggca ttctgtatga gtttacctgg aaccagttct gtgactggta tctcgagctg
     7021 accaagccgg taatgaacgg tggcaccgaa gcagaactgc gcggtactcg ccatacgctg
     7081 gtgactgtac tggaaggtct gctgcgcctc gcgcatccga tcattccgtt catcaccgaa
     7141 accatctggc agcgtgtgaa agtactttgc ggtatcactg ccgacaccat catgctgcag
     7201 ccgttcccgc agtacgatgc atctcaggtt gatgaagccg cactggccga caccgaatgg
     7261 ctgaaacagg cgatcgttgc ggtacgtaac atccgtgcag aaatgaacat cgcgccgggc
     7321 aaaccgctgg agctgctgct gcgtggttgc agcgcggatg cagaacgtcg cgtaaatgaa
     7381 aaccgtggct tcctgcaaac cctggcgcgt ctggaaagca tcaccgtgct gcctgccgat
     7441 gacaaaggtc cggtttccgt tacgaagatc atcgacggcg cagagctgct gatcccgatg
     7501 gctggcctca tcaacaaaga agatgagctg gcgcgtctgg cgaaagaagt ggcgaagatc
     7561 gaaggcgaaa tcagccgtat cgagaacaag ctggcgaatg aaggctttgt cgcccgcgca
     7621 ccggaagcgg tcatcgcgaa agagcgtgag aagctggaag gctatgcgga agcgaaagcg
     7681 aagctgattg aacagcaggc tgttatcgcc gcgctgtaat caaaacacag tgatgaaaac
     7741 gaaggccgga gcatgccccg gcctttttat ctcttacaga aatggtaaag aaggcataat
     7801 tccccgtgaa atactggcta agaagccttt atctggttgt tcttctttat tgattaaagg
     7861 aaggtcatcc aaatcgccca gcaaatacgt attttttgcc tgccagtcaa tcatccatat
     7921 tttcagcagt ggataagcca gaccgcccgt aatcccggat atcaccacca acgcacacat
     7981 gcgataaagc ataccgtggt atgttaaagt tgagcgaaaa cgaatacgcc catcattcag
     8041 tgacaggttg ctcataaaat gatttcgcaa cgacaccgtt aaataactgg tgctaacagc
     8101 aatcccaaaa taatagatta actgcgcgat tatcatttta cgttgcattt ccataaattg
     8161 ctgtaaattc tcaatatcat catttgcata cacagaacta tcatacgcat ttaatatttg
     8221 atcgaagata atataaccag caacagcgag aaaaggtaat aaagctaaaa atgcgaggat
     8281 ggcatatttt atacaatacg cagtatctaa tttcaccttg aaacgatgta taccgaaact
     8341 ggtattgctc cagagaaaac tcattaccag gctatataaa gtaccattaa aaataccaat
     8401 ggaaacaata ccaactattg ccatcagaac cacagataca ataacactac ttgaactatt
     8461 ggcatgtagc atctttgtcg agataaagaa aacagtcccc atcccaatgg ccattaaaat
     8521 cggcaagaaa aaggttaccc accagacccc tttcatagag caattaaaac taaatcttac
     8581 gccgttgaga ctggtcatca aggcctgata acgcagtcct tttgctgcca taaatataag
     8641 caaaaccaac agtgacaaag tcaaaacaca gccaataagc ggcatatctg ctgacactgt
     8701 cattaagatt gcgaaataga aaaaaacaaa aacaagacaa ctaaaaaaaa cattcccacc
     8761 agtaattcca taagaaaatc gttgtccgtt aacttccata ttagcataga gataacgctt
     8821 acatttcatt aatgcccatg gtaaatagat acctagcgtt ataatcgtta acaacacatt
     8881 caccacacaa ataagaaaat atctctcacc tgtaccatga aaaacaaacg aatgggacgg
     8941 aacatccatt tcattaataa cttgagccat aaaaattccc tgaattaaaa aatccattga
     9001 tataatttat agatacagac agtaaaatgt tctgccacgt tacaatactc ttatcagtaa
     9061 taggctggca aaaactttat tgctttataa gacgcttgcg cacgtaagaa gagagtggta
     9121 ttaaacaaat gtataattac tttattttta ttgggttaaa ctatgaataa cattgcgccg
     9181 caatcacctg taatgcgtcg cctgacgctg caggataatc ctgctatcgc ccgcgtcatt
     9241 cgtcaggtat ccgccgaata cggtcttacc gctgataaag gctacaccgt cgccgatccg
     9301 aatcttgacg agctgtatca agtgtatagt cagccgggtc atgcatattg ggtggttgag
     9361 tacgagggtg aagtggtcgg cggcggtggg attgcgccat tagcaggtag tgaatcggac
     9421 atttgcgaac tgcaaaagat gtattttctt ccggctattc gcggcaaagg gctggcaaaa
     9481 aaactggcct taaaggcgat ggaggaggca agagaaatgg gcttcaaacg ctgctatctg
     9541 gaaacgaccg cttttttgaa agaagcgatt ggtttgtatg aacatttagg ttttcaacat
     9601 atcgactatg cacttggctg cacgggccat gttgattgtg aagtgcggat gctacgcgaa
     9661 ctctaaaaaa atgcccgcta gtgtgtgcta tcgggcattt taatcaatta attagtggcg
     9721 aaccccgtca tcgtcttcat cgacaaaatc ttcatcgtcg ccgtcttcgc cgttgggatc
     9781 ttcaaagtaa gtgccccaac cgtcgtactc aacgtcaaat ttctccgcca gcgtcatcag
     9841 ttgttcaacc tgggcatcga tcagatcggc attcaacgcg cactcgctga ggatgtcgca
     9901 gcaaatcacg atatcaccat cttcaacttc cagctcttct ggatcggtca cttcgtaacc
     9961 gagtttaaac gcttcaactg ctgctttttc cagggtttcc agatcgtctg cggaaagatg
    10021 gtgttcgatg gtgtacagcg cgtccggatc gctgccatct tccagtaatt cttcaataat
    10081 caaacgtgtt tcttcacgct gttcttccag ttgttccggg tttgccatgg ctcattcctc
    10141 ttaatgtcct gccgatactc atattgtcac acaccactgg cattgcctcc acctttgtga
    10201 caaagattta tgctttagac ttgcaaatga ataatcatcc atataaattg aattttaatt
    10261 cattgagacg ttagccacag gagggatcta tgtccgggtt ttatcataag catttcctga
    10321 aattactcga tttcacgcca gctgaactca acagcctgct gcagttagcc gcgaagctga
    10381 aagccgataa gaaaagcggc aaagaagaag ccaaactcac tggtaaaaac atcgcgctca
    10441 tcttcgaaaa agactcgacc cgtacccgat gctctttcga agttgccgca tatgaccagg
    10501 gtgctcgcgt tacttatctc ggcccaagcg gcagtcagat tggtcataaa gagtcgatta
    10561 aagacactgc ccgcgtgctg ggtcgcatgt atgacggtat tcagtatcgc ggctatggtc
    10621 aggagattgt cgaaacactg gcggaatacg ctggcgtgcc ggtatggaac ggcctgacca
    10681 atgagtttca ccccacgcag ttgctggcgg atctcctcac catgcaggag catttacccg
    10741 gtaaaccatt caacgaaatg acgctggtct atgcaggtga cgcacgtaac aacatgggca
    10801 attcgatgct cgaagctgcg gcgcttaccg gtctggattt gcgtctggtc gcgccacagg
    10861 catgctggcc ggaagctgcg ctggttacgg aatgccgcgc cctggcacaa caaaatggcg
    10921 ggaatattac gctgacggaa gatgtagcta aaggcgttga aggtgctgac tttatctata
    10981 ccgatgtatg ggtgtcgatg ggtgaagcaa aagaaaaatg ggctgaacgc attgcattac
    11041 tgcgtgatta tcaggtgaac agcaagatga tgcagttgac cggtaacccg gaggtcaaat
    11101 tcctccactg cctgcccgcg ttccatgatg accaaacgac gcttggcaag aaaatggcgg
    11161 aagagtttgg cctacatggc ggaatggaag tgactgatga ggtcttcgaa tctgccgcca
    11221 gcattgtttt tgatcaggcg gaaaaccgca tgcacaccat caaagcggtg atggtcgcga
    11281 cactcagtaa ataattgata tcgctataaa gatgccggga aatacagaaa catcccggcg
    11341 tcaaattatt ttatgcagtg atagtttttt tcttccagcc aaatttttct attgctatac
    11401 gtaaattttc cggaggattt agtgtattta aacgctgttg gtcaataaat ggcgatacat
    11461 taacctccga tttcaagata gtttgcatca ttctaaacct aagttttaat tcattattaa
    11521 gtttttcttc aataacatct tttggttgat tttt
//
LOCUS       DAZKIF010000132        11461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZKIF010000132 DAZKIF010000000
VERSION     DAZKIF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.133"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            368..1756
                     /gene="narU"
                     /locus_tag="ACHOTW_003838"
     CDS             368..1756
                     /gene="narU"
                     /locus_tag="ACHOTW_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP9845862.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1838..5578
                     /gene="narZ"
                     /locus_tag="ACHOTW_003839"
     CDS             1838..5578
                     /gene="narZ"
                     /locus_tag="ACHOTW_003839"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP9845863.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTSSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5575..7119
                     /gene="narH"
                     /locus_tag="ACHOTW_003840"
     CDS             5575..7119
                     /gene="narH"
                     /locus_tag="ACHOTW_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9845864.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7119..7814
                     /gene="narW"
                     /locus_tag="ACHOTW_003841"
     CDS             7119..7814
                     /gene="narW"
                     /locus_tag="ACHOTW_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9845865.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7811..8491
                     /gene="narI"
                     /locus_tag="ACHOTW_003842"
     CDS             7811..8491
                     /gene="narI"
                     /locus_tag="ACHOTW_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9845866.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8570..9463
                     /gene="yddE"
                     /locus_tag="ACHOTW_003843"
     CDS             8570..9463
                     /gene="yddE"
                     /locus_tag="ACHOTW_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP9845867.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9559..10403)
                     /gene="nhoA"
                     /locus_tag="ACHOTW_003844"
                     /pseudo
     CDS             complement(9559..10403)
                     /gene="nhoA"
                     /locus_tag="ACHOTW_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10702..11145
                     /locus_tag="ACHOTW_003845"
     CDS             10702..11145
                     /locus_tag="ACHOTW_003845"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9845868.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11149..11376)
                     /gene="pptA"
                     /locus_tag="ACHOTW_003846"
     CDS             complement(11149..11376)
                     /gene="pptA"
                     /locus_tag="ACHOTW_003846"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFP9845869.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
BASE COUNT         2702 a         2973 c         3015 g         2771 t
ORIGIN      
        1 aggaaccgat agactttctg gacagatcgt tttcctccag ctcatggcct ttttatcact
       61 aattctataa gtgtctggtc ttgttaaaaa tatagttaat atacaaccct ttggattcat
      121 catctgaagg gttgaatcat gacatgcgta catagcttaa ccccgtttaa tggttagctt
      181 gttagttaca tttagtaaca cataactggc tgcttaatcc ccattctgcc cccgcggtta
      241 cgctttctta tctattcttt caatcagcac ttcacccatt tcatccttaa ttctaataac
      301 tggctattgc gccttacctc ctgttttgtc tttttttcat taccaatgtg tacatgtgag
      361 gaacaatatg gcactgcaaa atgagaaaaa tagtcgttat cttttacgcg actggaaacc
      421 agaaaatccg gccttctgga aaaataaagg aaaacatatt gctcgaagaa acctctggat
      481 atcagtcagt tgtctacttc ttgccttctg tgtctggatg ctatttagcg cagttaccgt
      541 taatctcaat aaaatcggtt ttaatttcac taccgatcaa ctctttttat taaccgcatt
      601 accctccgtt tctggcgcat tattacgtgt cccctactcc tttatggtgc ctatattcgg
      661 tggacgtcga tggacggttt ttagtactgc aatcctgatt attccttgcg tctggctcgg
      721 aattgccgtg cagaatccga atactccttt tgggatattt atcgttatcg ctttgctatg
      781 cggttttgca ggtgcgaact ttgcttcgag catgggcaat atcagtttct tctttccaaa
      841 agccaggcaa ggtagcgctc ttgggattaa tggcggatta ggaaacttag gtgtaagtgt
      901 aatgcagctg gttgcaccgc tggtcatttt tgtacccgta tttgcctttc tcggcgtcaa
      961 tggagtaccg caggccgacg gttcggtgat gtcgctggcg aatgccgcat ggatttgggt
     1021 accgctacta gcgattgcca cgatcgccgc ctggtcaggg atgaatgata tcgccagttc
     1081 acgcgcgtca attgccgacc agctccctgt cttacaacgc ctgcatctct ggctgctgag
     1141 cctgctttac cttgccacct tcggttcgtt tatcggtttt tctgcgggtt tcgccatgct
     1201 ggcaaaaacc cagttcccgg atgtgaatat tctgcgcctg gcgttctttg gcccatttat
     1261 cggtgccatc gcgcggtcgg tgggtggtgc tatttccgat aaattcggcg gcgtgcgggt
     1321 gacgttgatc aactttattt ttatggcgat tttcagcgcc ctgctgttcc ttaccttacc
     1381 gggcacaggc tccggtaact tcatcgcctt ttacgccgta tttatggggc tgtttctgac
     1441 cgcgggtctg ggaagtggtt ctactttcca gatgatcgcc gtcatctttc gccagataac
     1501 catttatcgg atgaagatga aaggcggtag tgatgagcaa gctcagagag aagccgtcac
     1561 cgaaacggcg gcggctctgg gctttatctc agccatcggc gcagtgggcg gcttttttat
     1621 tccgcaggca tttggcatgt cactcaatat gaccggctct ccagtgggcg cgatgaaagt
     1681 gtttttaatc ttctacatcg tttgtgtgct actgacctgg ctggtttatg gtcggcggaa
     1741 gttcagccaa aaataagaga tcgatgcctg atgtacaacc gtgtcgcatc cgacaaggca
     1801 tcagatccca attcattcct ggagcaggag tcatgtcatg agtaaacttt tggatcgctt
     1861 tcgctacttc aaacaaaagg gcgatacctt tgccgatggt cacggacagg tgatgcatag
     1921 caaccgcgac tgggaggaca gctatcgcca gcgttggcag ttcgacaaaa tcgtgcgttc
     1981 cacccacggt gttaactgta caagctcctg tagctggaaa atctacgtta aaaatggtct
     2041 ggtgacctgg gaaatccaac agaccgacta cccgcgcact cgccctgacc tgcccaatca
     2101 tgaacctcgc ggctgcccgc gtggcgcaag ttactcctgg tatctttaca gcgctaaccg
     2161 cctgaaatac ccgctcattc gtaaacgact gattgaactg tggcgcgaag ccctcaaaca
     2221 acacagcgat ccggtactgg cgtgggcatc gattatgaac gatccgcaaa agagcctgag
     2281 ctacaaacaa gtgcgtgggc gcggcgggtt tatccgctcc aactggccgg aactaaacca
     2341 gctgattgcc gccgctaacg tctggactat caaaacctac ggcccggatc gcgttgccgg
     2401 tttctcgccg atcccggcga tgtcgatggt ttcttacgcc gccggaacac gttatctgtc
     2461 actattaggt ggcacctgtt taagcttcta cgactggtat tgcgacctgc cgcccgcctc
     2521 gccgatgacc tggggcgagc aaaccgacgt accggaatct gccgactggt ataactccag
     2581 ttacatcatc gcgtgggggt ctaacgtacc gcagacacgt acgccggacg cccatttctt
     2641 taccgaagta cgctacaaag gcactaaaac catcgccatt acccctgact actctgaagt
     2701 ggccaaattg tgcgaccagt ggctggcacc aaaacaaggc actgatagcg ccctggcgat
     2761 ggcaatgggc catgtgattt taaaagagtt tcatctcgat aatcccagcg actactttat
     2821 caactactgc cgccgctata gcgacatgcc gatgctggta atgctggaac ctcgcgacga
     2881 tggtagctac gtacccgggc gcatgatccg cgcgtctgac ctggtagatg gactaggcga
     2941 aagcaataat ccgcagtgga aaaccgtggc ggttaatacc gcaggtgaat tggtagtgcc
     3001 gaatggttcg attggtttcc gctggggaga aaaaggcaaa tggaatctgg aatccattgc
     3061 cgccggtaag gaaaccgaat tgtcgttaac cctgctcggt caacatgacg ccgttgcagg
     3121 cgtggctttc ccctactttg gcggcatcga aaatccgcat tttcgcagcg tcaaacacaa
     3181 tcctgtcctg gtgcgtcaat tacctgttaa aaacctgaca ttagccggtg gcagcacctg
     3241 tccagtggtc agcgtttatg atttggtact ggcgaattac ggcctcgatc gcgggctgga
     3301 agatgaaaac agcgcgaaag attacgctga aatcaaaccg tacaccccag cctggggtga
     3361 gcaaattacc ggcgtgccgc gccagtatat tgaaaccatc gctcgtgaat ttgccgatac
     3421 tgcccataaa acgcatgggc gctcgatgat tatcctcggc gcaggtgtta accactggta
     3481 tcacatggac atgaactacc ggggaatgat caatatgctg atcttctgcg gttgtgtcgg
     3541 acaaagcggt ggcggctggg cacactatgt cggtcaggaa aaactgcgcc cgcaaaccgg
     3601 ctggttgccg ctggcctttg cgctcgactg gaaccgacca ccgcgccaga tgaacagcac
     3661 ctcgtttttc tacaatcatt ccagccaatg gcgctatgaa aaagtctctg cgcaggagtt
     3721 actttcaccg cttgccgatg ccagtaagta cagcggtcat ctgattgatt ttaacgttcg
     3781 cgccgaacgc atgggctggc taccttctgc gccgcagttg gggcgtaacc cgctcgggat
     3841 taaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc aggcgctgaa
     3901 atcgggcgat ttacgtatgg cctgcgaaca accagatagc ggcagcaatc atccgcgtaa
     3961 tttgtttgtc tggcgttcta acctgcttgg ctcctccggc aaaggccacg agtatatgca
     4021 gaagtatctg ctggggaccg aaagcgggat tcagggcgag gaactcggtg ccagcgacgg
     4081 gatcaaaccg gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc tcgacctgct
     4141 ggtgacgctc gacttccgca tgtccagtac ctgcctgttc tccgatatcg ttctgcccac
     4201 cgccacctgg tatgaaaaag acgatatgaa cacctcggat atgcatccat ttattcatcc
     4261 gctttctgcg gcggtcgatc cggcctggga atcacgcagc gactgggaaa tctacaaagg
     4321 tattgccaaa gcattttcgc aagtgtgcgt gggccatctt ggcaaagaaa ccgacgtggt
     4381 attacaacca ctgctgcatg actctccggc agagctctca cagccgtgtg aagtgctcga
     4441 ctggcgcaaa ggcgaatgcg atctgagccc gggtaaaacc gcgccgaata ttgtggcggt
     4501 ggagcgcgac taccctgcta cgtatgaacg ctttacctcg ctcgggccat tgatggacaa
     4561 acttggcaac ggcggtaaag ggatttcgtg gaatacgcag gatgaaatcg atttcctcgg
     4621 taaactcaat tacaccaagc gtgatggccc agcgcagggg cgtccgctga ttgacaccgc
     4681 cattgacgct tcagaagtga ttctggcact ggcaccagaa accaacggtc atgttgcagt
     4741 taaagcgtgg caggcgctgg gcgagatcac cggacgcgaa catacccatc tggcgctgca
     4801 caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta aaattatctc
     4861 cagccccaca tggtctggtc tggaaagcga tcacgtctcc tataatgcgg gatacaccaa
     4921 cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcagctct atcaggatca
     4981 tccgtggatg cgtgcttttg gtgaaagcct ggtggcatat cgcccgccta tcgacacccg
     5041 tagcgtcagt gagatgcgcc agatcccgcc aaacggcttc ccggaaaaag cacttaactt
     5101 cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc tgctaatgct
     5161 gacgctctct cgcggtggac cgattgtctg gatcagcgaa accgatgccc gtgaactaac
     5221 cattgtcgat aacgactggg tggaagtgtt taacgccaat ggcgcgctga cggcccgcgc
     5281 ggtggtcagc caacgtgtac cgccgggtat gaccatgatg tatcacgctc aggaacgcat
     5341 tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggtattcata actcggtcac
     5401 ccgcatttgc ccgaaaccaa cgcatatgat tggcggttac gcgcagctgg cctggggctt
     5461 taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc gcaagatgaa
     5521 gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga aaaaatgaaa
     5581 atccgttcgc aagtcggcat ggtgcttaat ctcgataaat gtatcggctg ccatacctgt
     5641 tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc atggtttaac
     5701 aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca ggaagagtgg
     5761 caaggcggct gggtccgcga tgtgaatggc aagatacgcc cgcgtctggg cagcaagatg
     5821 ggtgtaataa ccaaaatctt cgccaacccg gtggtgccgc agattgatga ttactacgaa
     5881 cctttcacct tcgactacga acatttgcat agcgcaccgg aaggcaaaca tatccctact
     5941 gctcgcccgc gttcgctgat tgacggtaag cggatggaca aagtgatctg ggggccaaac
     6001 tgggaagaac tgctgggcgg cgagtttgaa aaacgtgccc gcgaccgcaa cttcgaggcc
     6061 atgcaaaagg agatgtacgg gcagtttgaa aacaccttca tgatgtacct gccgcgcctg
     6121 tgcgaacact gcctcaatcc cagctgcgtg gcgacctgcc caagcggcgc tatctacaaa
     6181 cgcgaagaag acggcattgt gctgattgat caggataaat gccgtggctg gcgtttgtgc
     6241 attagcggtt gtccgtacaa aaaaatctac ttcaactgga aaagcggcaa gtcagaaaaa
     6301 tgtatcttct gttacccacg aattgagtcc ggtcaaccga ccgtgtgctc agaaacctgc
     6361 gtgggtcgca tccgatatct gggcgtgctg ctttacgacg ctgaccgcat cgaggaagcg
     6421 gcgagcaccg agcgcgaggt tgacctctat gaacgccagt gcgaagtgtt cctcgatccg
     6481 cacgatccct cagtgatcga ggaagccctg aaacaaggta ttccacaaaa tgtgattgac
     6541 gctgcccagc gttcgccagt ctacaaaatg gcgatggact ggaaactggc actaccgctg
     6601 caccctgaat accgcaccct gccgatggtc tggtacgttc ctccgctgtc accgattcag
     6661 tcctacgcag atgcgggcgg tttgccgaaa agcgaaggcg tgctgcccgc catcgaaagc
     6721 ctgcgtattc cggtgcaata tctcgccaat atgttgagtg ccggcgatac cggtccggta
     6781 ctgcgggcgc tgaaacggat gatggcgatg cgccactata tgcgttcaca aaccgtggaa
     6841 ggcgttactg atactcgtgc catcgacgaa gtaggcctga gcatcgccca ggtcgaagaa
     6901 atgtatcgct atctcgccat tgccaactat gaagatcgtt ttgttatccc gaccagccat
     6961 cgggaaatgg cgggcgatgc cttcgcagaa cgcaatggct gcggttttac ctttggtgac
     7021 ggttgtcacg gctcggacag caaattcaac ctgttcaaca gtagccgtat cgatgccatc
     7081 aacatcaccg aagtgcgcga caaagcggag ggcgaataat gcagatcctc aaagtgatcg
     7141 gcctgttgat ggagtatccg gacgagatgt tgtgggaatg caaggaggac gcgctggcgt
     7201 tgatccgccg cgatgcgccg atgcttacgg atttcacccg caacctgctt aacgcgccgc
     7261 tgctggataa acaggccgaa tggtgcgaag tgtttgaccg cgggcgcacc acgtcgctgc
     7321 tgctgttcga acatgttcat gccgagtccc gcgatcgcgg ccaggcaatg gtggacctgc
     7381 tggcggagta tgaaaaggtc ggcctgcagc tggattgtcg ggaactgccc gattatctac
     7441 cgctgtatct ggagtattta agcgtactgc ctgacgatca ggcgaaagaa ggattgctca
     7501 acgttgcgcc gatcctcgcc ctgcttggcg gtcgcttaaa acaacgcgag gcaccgtggt
     7561 acgcgttgtt tgatgctctg ctgcaactgg cgggaagtac actttcaagt gacagtgtca
     7621 caaaacaagt aaacagcgaa gagcgcgatg acacccgcca ggcgcttgat gcagtgtggg
     7681 aagaggaaca ggtgaagttt attgaagata acgccacggc gtgtgacagc tcgccgctta
     7741 atcaatatca gcgacgcttt agtcaggatg tcgcgccgca atatgtcgac atcagtgcgg
     7801 gaggtgggaa atgattcagt atctgaacgt ctttttttac gatatctacc cgtacatttg
     7861 tgcgacggtg tttttcctcg gcagctggct gcgctacgac tacgggcagt acacctggcg
     7921 cgcctcctca agtcagatgc tcgataaacg cgggatggtg atatggtcga atctgttcca
     7981 tatcggcatt ttggggatct tcttcgggca cctgttcggc atgttaacgc cacactggat
     8041 gtacgcgtgg tttttgccag tggcagcgaa acaactaatg gcgatggtgc tcggtggtat
     8101 ttgcggtgtg ttgacgttga ttggcggcgc aggactgctg tggcgcaggc tgacaaatca
     8161 gcgcgtgcgt gccacttcca ctacgccgga tatcatcatc atgagcattt tgctgatcca
     8221 gtgtctgttg gggctaagta ccataccgtt ctcagcacag tatcctgatg gtagcgaaat
     8281 gatgaagctg gtgggttggg cgcaaagcat tgtcactttc cgtggtggat cgtcagagat
     8341 gcttaacggt gtagcgttcg tcttccgcct gcatctggtg ttgggaatga ccatcttctt
     8401 gctcttccca ttcacccgac tggtgcatgt gtggagcgcc ccgtttgagt actttactcg
     8461 tcggtatcag attgtgcgct cgcggcgata atttcaccct ggccggatag cccttcgctc
     8521 tccggccact tgcaggctat gatagttctc cagtcacagg agaacgttaa tgaaaccgca
     8581 ggtttaccac gtcgatgctt ttacctctca accatttcgc ggcaattctg ccggtgttgt
     8641 tttccccgcc gataatctta gcgaagcgca aatgcagctt atcgcccgcg aattaggtca
     8701 ttcggaaacc gcttttctgc tgcacagcga cgacagcgat gtgcgcatcc gctactttac
     8761 gccaacagtt gaagtgccta tttgcggtca cgcgacagta gctgcgcact atgtacgtgc
     8821 gaaggtgtta ggtttaggaa attgcacggt ctggcaaaca tcgctggcag gaaaacatcg
     8881 cgtgactatc gaaaagcacc acgatggtta tcgtatttcg ctggaacaag gtacgccagg
     8941 ctttgagcca ccgctggaag gtgaaacacg tgcggcgatt atcaacgcgc ttcatctcac
     9001 tgaggacgat attctgccag gcttgccaat tcaggtggca accacaggac actcaaaagt
     9061 gatgatccca ctgaaaccgg aagtggatat cgacgccctt tcgcctgacc ttaatgcgct
     9121 gaccgctatc agcaaacaga ttggctgcaa tggtttcttc ccgttccaga tccgcccagg
     9181 caaaaacgaa accgatggtc gcatgttctc gcctgcgatt ggtattgtgg aagatccggt
     9241 gactggcaat gccaatggcc cgatgggtgc atggttggta catcacaacg tattgcccca
     9301 cgatggcaac gtgttgcgtg ttaaaggcca tcaggggcgc gcattggggc gtgacggtat
     9361 gattgaagtg acggtgacaa ttcgtgataa ccaaccggaa aaagtcacca tttctggcgc
     9421 ggcggtgatt ttgtttcatg ccgaatgggc aattaaactc tgaaaccgtt gcctgatgcg
     9481 cttcgcctgt caggtctaca accgggctaa tgtatgccgg ataaggcgtt tacgccgcat
     9541 ccggcaacct gacataaatt attttcccgc ctccgggtgc gtatcaaacg ccgccatcac
     9601 cagcgctaac tcatccacgg taaagccatg tttcgcatca tccacgccca gaccaaactg
     9661 ttcttgcatc acagcatata acgacgccac atccggtaga tttcgctgct ccaccgcgtg
     9721 cccattttca taatgggaaa atgaaaattg gtcagtgtca gcttgccgcc gtccggcaaa
     9781 tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc cgaccagaaa
     9841 ttacccatca cataatcgct ttgttgctgc tcgcagagat caaaacggta catcgactgc
     9901 cagtgctgat gatgattaaa ctgcaacacc caatcatcac cctcctgcaa caaccgatac
     9961 tctccgtgtg gcgtggtctg cacaagatcg ggaactaaac gaatcggcgc ggttagcgtc
    10021 tgcccaccga aaccgacatc agcaatccat ttttcctctt ccagttccac caacagcaaa
    10081 cgatgggtgc gcggcggtaa tgctggcgga tttgataaca ctacccgccc taacaagctg
    10141 cgaacgttaa accccagctc gcgtaacacc cgctcaaaaa cgccattctg ctcaaaacag
    10201 taaccgccac gacgggctat caccagtttc tcttccagcg attgatcatc aagctgcatt
    10261 tccctcggca gcaaaacgtc gaggttttca aacggaatgg tgcaattgtg tttcaggtgc
    10321 aatgcacgaa gcgtatcaat attgaccgca gcagctcccg accagttaat acgggcaaaa
    10381 tagtgattca gaatgggcgt catcaggcgt ttcccttagt ggttttctca acttataacc
    10441 tggaatagac gccgcaccgg ctattttgtg cgatcgcgga catgcatcac gttcgcaaat
    10501 cgccaatccc gttaatttcg ttacttattg cgttacggac tacgcttaaa aaaaccttct
    10561 tcgcgaggcg ataccatgag ccgattcatc cccatagaat tacaccatgc cagccggctg
    10621 ttaaatcatg gcccaactgt catgatcacc agtttcgatg agcaatccca gcggcgtaac
    10681 attatggctg cagcctgatc aatgccggtg gagtttgaac ctccacgcgt gacgattgtg
    10741 gtagataaat cgacatggac cagggagttg attgaacgta acggtaaatt tggcatcgtt
    10801 atcccgggcg ttgcagcaac taactggacg tgggcagtgg gaagtgtgtc gggtcgtgaa
    10861 gaagataaat ttaattgcta tggcattccg gttgtgagag gcccggtatt tggtttgcct
    10921 ctggtcgagg aaaaatgtct ggcgtggatg gagtgtcgat tgctacctgc gacttctgcg
    10981 caagaagaat acgacacgct gtttggcgaa gtagtatcag cagcggcaga cgcacgggta
    11041 tttgtcgaag gccgctggca gtttgatgat gataagctca atacgttgca tcatttaggt
    11101 gctgggacgt ttgttaccag cggcaagcgt gttacggcgg gttaagcatt acatgctgta
    11161 accgggtttc tttatcaaag cctccatttg gggcgcgatt tcggcatccc agatagcttg
    11221 ccaagattct ggttgaatct gttgtagagc aatgcttatc gaactgtctt tactgttcag
    11281 atggcgaata ataacgtcgg taatatctgc agcaagtgct gctttttgtt gttcgtccag
    11341 ttcacgcgga aaacatttaa tgtctatgtg cggcatgatt acgctccttt tgaaagatgt
    11401 cagtaaaatt aacacacatt gtgaatgtcg tgtgtccggt aaagcgtttt tacgacaata
    11461 a
//
LOCUS       DAZKIF010000133        11403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZKIF010000133 DAZKIF010000000
VERSION     DAZKIF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.134"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            291..1862
                     /gene="garD"
                     /locus_tag="ACHOTW_003847"
     CDS             291..1862
                     /gene="garD"
                     /locus_tag="ACHOTW_003847"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP9845870.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            2011..2346
                     /gene="prlF"
                     /locus_tag="ACHOTW_003848"
     CDS             2011..2346
                     /gene="prlF"
                     /locus_tag="ACHOTW_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP9845871.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2346..2810
                     /gene="yhaV"
                     /locus_tag="ACHOTW_003849"
     CDS             2346..2810
                     /gene="yhaV"
                     /locus_tag="ACHOTW_003849"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP9845872.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2865..3674)
                     /gene="agaR"
                     /locus_tag="ACHOTW_003850"
     CDS             complement(2865..3674)
                     /gene="agaR"
                     /locus_tag="ACHOTW_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP9845873.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3923..5203
                     /gene="kbaZ"
                     /locus_tag="ACHOTW_003851"
     CDS             3923..5203
                     /gene="kbaZ"
                     /locus_tag="ACHOTW_003851"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP9845874.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5226..5699
                     /gene="agaV"
                     /locus_tag="ACHOTW_003852"
     CDS             5226..5699
                     /gene="agaV"
                     /locus_tag="ACHOTW_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP9845875.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            5710..6489
                     /gene="agaW"
                     /locus_tag="ACHOTW_003853"
     CDS             5710..6489
                     /gene="agaW"
                     /locus_tag="ACHOTW_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP9845876.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6479..7357
                     /gene="agaE"
                     /locus_tag="ACHOTW_003854"
     CDS             6479..7357
                     /gene="agaE"
                     /locus_tag="ACHOTW_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP9845877.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7375..7809
                     /gene="agaF"
                     /locus_tag="ACHOTW_003855"
     CDS             7375..7809
                     /gene="agaF"
                     /locus_tag="ACHOTW_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP9845878.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7806..8939
                     /gene="nagA"
                     /locus_tag="ACHOTW_003856"
     CDS             7806..8939
                     /gene="nagA"
                     /locus_tag="ACHOTW_003856"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9845879.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9290..10444
                     /locus_tag="ACHOTW_003857"
     CDS             9290..10444
                     /locus_tag="ACHOTW_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP9845880.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10457..11317
                     /gene="kbaY"
                     /locus_tag="ACHOTW_003858"
     CDS             10457..11317
                     /gene="kbaY"
                     /locus_tag="ACHOTW_003858"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP9845881.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2733 a         2931 c         3018 g         2721 t
ORIGIN      
        1 tgaactcggg aattagtata agcagcgcga gaataataat cattgtgcaa atgctaattt
       61 aattaatact atttaaatat tattttgagc atatgcacat aaggttgcgc gctaaagcac
      121 agatttgcgc tttaccttgc cgggcggcat tgcaatccct gaaatgattg acattgatca
      181 catttctgcg tttaaactcc tgacattctt atttcaccca atgaagtcat ttatttttaa
      241 atgagaccag gtcctcattt taataacctc tggctggaga atattgcaca atggccaaca
      301 tcgaaatcag acaagaaacg ccaactgcgt tttatataaa agttcacgac acagataatg
      361 tggcaattat tgttaatgat aatggcctga aagcaggaac gcgttttccg gatgggctgg
      421 aattaattga acatattccc caggggcata aagtcgcatt gctcgacatt ccggctaatg
      481 gtgaaattat tcgttatggc gaagtgattg gttacgccgt gcgtgcaatc ccacgcggaa
      541 gctggatcga cgaatcaatg gttgtactgc cgaaagcgcc gccgttacac acgctgccac
      601 tggcaaccaa agtcccggaa cccttaccgc cgctggaagg atacaccttt gagggctatc
      661 gcaatgccga tggcagcgtg ggcaccaaaa acctgctcgg catcaccacc agcgtccact
      721 gtgtggcagg cgtggtggac tacgtagtaa aaatcattga acgcgatctg ctaccgaaat
      781 acccgaacgt cgatggcgtg gtggggctga atcatttgta cggttgtggc gtggcgatta
      841 acgcaccggc ggcagttgta cctatccgta ccattcacaa tatttcgctg aatcctaact
      901 ttggcggcga agtaatggtg attggcctgg gttgtgaaaa gttgcagcct gagcgcctgc
      961 tgactggaac ggatgatgtg caagctattc cagtagaaag cgccagcatt gtcagtttgc
     1021 aggatgaaaa gcatgtcggt tttcagtcca tggtcgagga tattttgcag gtcgccgaac
     1081 gccatctaca aaaactgaat caacgtcagc gagaaacctg tccggcttca gaactggttg
     1141 tcggcatgca gtgcggtggc agcgatgcct tttctggcgt aacggcaaac ccggcggttg
     1201 ggtatgcgtc tgatctactg gtgcgctgcg gcgcaacggt gatgttctca gaagtcacgg
     1261 aagtgcgtga cgcgatccat ctgctgacac cacgcgcagt gaacgaagag gtcggcaaac
     1321 ggctgctgga ggagatggag tggtacgata actatctcaa tatgggaaaa accgaccgca
     1381 gcgccaaccc ttcgccgggc aacaagaaag gcggtctggc aaatgtagtg gagaaagcgc
     1441 tcggctccat tgctaaatcg ggtaaaagtg caattgttga agtgctgtcg cccggtcaac
     1501 gcccgactaa acgcggatta atttacgccg cgacgccagc cagcgatttt gtctgtggca
     1561 cgcaacaggt ggcttcgggt atcaccgtgc aagtgtttac gaccggtcgt ggtacgccgt
     1621 acggcctgat ggcggtaccc gtcattaaaa tggcgacccg caccgagctg gcgaaccgct
     1681 ggtttgattt aatggatatt aacgcgggca ctatcgccac cggcgaagaa accattgaag
     1741 aggtgggctg gaagttgttc cactttattc tcgacgtcgc cagcgggaag aagaaaacct
     1801 tctcggatca atggggactg cataaccagc tggcggtgtt taacccagca ccggtgacct
     1861 gatttctttt ggaacgtccc tcgcaaaaca tggccttagt gccatgtttt tattgtttaa
     1921 agcccccacg tccattaata atgcatttgc attaccttag ttcaagctta taatttgagc
     1981 agaaaacagg ctgtaaaagg acagtgaatc atgcccgcta atgctcgctc tcacgctgta
     2041 ctgaccactg aatcaaaggt cacgatacgc ggacaaacaa ctatccccgc gccagtgcgt
     2101 gaggccttaa aactgaagcc aggccaggac agcattcatt acgaaattct gcctggtggg
     2161 caagtattta tgtgccgact gggagatgag caggaggatc atactatgaa tgcatttttg
     2221 cgttttctgg atacagatat ccagaacaac ccgcaaaaaa ctcgtccatt caacattcaa
     2281 caaggaaaga aacttgtcgc tggcatggac gtcaacattg atgatgagat tggcgacgac
     2341 gaataatgga ttttccacaa agggttaatg gttgggcgct atatgctcat ccctgttttc
     2401 aggaaaccta cgacgcttta gttgccgaag tcgagacatt aaagggaaaa gatcctgaaa
     2461 attatcagag aaaagctgcc acaaagttat tggcggtggt ccataaagtg attgaggagc
     2521 atatcacggt caatccatca tcaccggcat tccgtcatgg caagtcgtta ggctctggga
     2581 aaaataaaga ctggtcacgg gtaaaatttg gtgctggtcg ctatcgtctc ttctttcgtt
     2641 acagtgaaaa agagaaagtc atcattctgg gatggatgaa cgatgaaaac acgctgcgca
     2701 cctacggtaa aaaaacagat gcctataccg tattcagcaa aatgttaaaa agaggacatc
     2761 ctcctgccga ctgggaaacc ctcacccgag agacagaaga aacccattga tggtgttcac
     2821 aatgcccgcc gaacgcattc gtgttgacca gcgggcaccc tttctcactc cccgaccaga
     2881 atcacttcaa ccccagcctt tcgcagccct tccaggctat ccgcaggaat gccttcatca
     2941 acaatgatca tgtcgatacg ttgagtatca atgatcttat gtaaactgga acgattgaat
     3001 ttactggaat cggtgaccac gatgatccgt tccgcaactt cgcacatccg gcggtttaaa
     3061 cgggcttcat cttcattatg cgtgctgacg ccgcgctcca gatcgatcgc atctacacca
     3121 agaaacagca tatcgaagtg gtaattttgc agcgattgct cagcctgatc gccgtaaaaa
     3181 gattgcgact gacggcgcaa atgcccgccg gtcatcagca gctcaacgcc ttccgcttcc
     3241 agcaacgcat tagccacgtt cataccgttg gtcatcgcaa ttacgtcagt gtgcttgcgc
     3301 atcagacgag caatctcaaa agtggtggtc ccggaatcga ggatcacccg atgacctggc
     3361 tgaatcaact caacggcagc tttcgcaacg ctgcgtttca ttgcggtgtt cagcgcgctt
     3421 ttatcttcca ctgatggctc gactgacggc gttgtgctat cgcagatcaa cgcgccacca
     3481 taggcacgca cagcgatccc ctgcttttcc agaaacgcca gatcgttgcg gatcgtcacg
     3541 gtagatacgc catacaatgc cgacagatcg ttaacctgca cactcccttg ctgtcgcaga
     3601 cgctgaatga tctgttctcg tcgctcgctg gtgcctgtca ctcgcttctc acctgaagcg
     3661 tcggtattac tcatagtaag tcctttcgta aaactttcgt ttcatttcgt tttgcctatt
     3721 aacgcctttc tattaagcaa atgcaagccc accttgccca ttgacgcaag ctactctcgt
     3781 ttcagtgact ttcattatgt ttcttttgtg aatcagatca gaaaactatt atctttcgtt
     3841 ttatttttat ctcaccatga cgcagtatca actgaaacaa aacgaaagat taatatcgca
     3901 gtaatctgaa ctggagagga aagtgaaaca tctgacagaa atggtgagac agcacaaagc
     3961 gggcaaaaca aatggaattt atgccgtttg ttccgcacat ccgctggtgc tggaggctgc
     4021 aatccgctac gccagtgcaa accaaacgcc gttactgatt gaagcaacct ccaatcaggt
     4081 agaccagttc ggcggttata ccggaatgac gcccgccgat tttcgcggct ttgtttgtca
     4141 gctcgccgac tcgttgaatt tcccgcagga tgcgttgatt ctgggtggtg accatctggg
     4201 gccaaaccgc tggcaaaacc tgccggccgc tcaggcaatg gccaatgccg atgatttgat
     4261 taaaagctac gttgcggcag gattcaaaaa aatccacctt gattgcagca tgtcctgtca
     4321 ggacgatccg attcccttaa ctgatgacat cgtggctgaa cgcgccgccc gtctggcgaa
     4381 agtggcggaa gaaacctgtc ttgaacactt tggcgaagcc gatctggagt atgtcattgg
     4441 taccgaagtg ccggtacctg gcggcgcgca tgaaacctta agcgagctgg cggtcaccac
     4501 gccggatgcc gcccgcgcca cgctggaagc ccatcgtcac gcctttgaaa agcaaggttt
     4561 gaatgccatc tggccacgca tcattgccct ggtggttcaa cccggcgtcg aattcgatca
     4621 caccaacgtt attgattatc agcccgccaa agcgaccgcc ttaagccaga tggtcgaaag
     4681 ctacgaaacg ctgattttcg aagcgcactc taccgattat caaacgccgc aatcgctgcg
     4741 ccagctggtg attgaccact ttgccattct gaaagttggc ccggcgctga ccttcgccct
     4801 gcgtgaagct ctgttctctc tggcagcgat tgaagaagaa ctggtgccag caaaagcctg
     4861 ttctggtctg cgtcaggtgc tggaaaacgt gatgctcaac cgcccggaat actggcaaag
     4921 ccactaccac ggtgacggca acgcgcgtcg tctggcgcgt ggttatagct actcggatcg
     4981 cgtgcgctat tactggccgg acagccagat tgatgacgct ttcgctcatc tggtacgtaa
     5041 tctggcggat tcaccaattc cgctgccgct gatcagccag tatctgccgc tgcagtacgt
     5101 gaaagttcgc tccggcgagc tgcagccaac gccacgggaa ctcattatca accatattca
     5161 ggacatcctg gcgcagtacc acacagcctg tgaaggccaa taagcaaaac aaagaggaac
     5221 acgctatgcc aaatattgtt ttaagccgga ttgatgaacg cttgattcac ggtcaggtcg
     5281 gcgttcagtg ggtcggattt gcgggggcaa atctggtgct ggtcgccaac gatgaggttg
     5341 ccgaagatcc ggtacaacaa aacctgatgg aaatggtact ggcagaaggg atcgccgtac
     5401 gtttctggac gctgcaaaaa gttatcgaca acattcatcg cgccgccgat cgacagaaaa
     5461 tcctgctggt ttgtaaaaca cccgccgatt tcctgacgct ggtgaaaggt ggcgttccgg
     5521 tgaatcgcat taacgttggc aatatgcact acgccaatgg caagcaacaa atcgccaaaa
     5581 cggtttctgt ggatgcgggc gatatcgcag catttaacga cctgaaagcc gctggggtgg
     5641 aatgcttcgt tcagggcatc ccgacagagc ctgctgtgga cctctttaaa ttactttgag
     5701 ggattcatca tggaaatcag tctgttgcag gcattcgcgt tgggcattat cgcctttatc
     5761 gctggcctgg atatgtttaa cggcttaacc catatgcacc gcccagtggt cctcggcccg
     5821 ttagtcgggc tggtacttgg cgatctgcat accggtattt taaccggcgg tacgctggaa
     5881 ctggtgtgga tggggctggc cccgctggcg ggcgcacagc cgcctaatgt gattatcggt
     5941 actatcgtcg gcacggcgtt tgccattact actggcgtga aacccgatgt cgcagtcggt
     6001 gtcgccgtac ctttcgctgt cgcagtacag atggggatta ccttcctgtt ctcggtgatg
     6061 tctggcgtga tgtctcgctg cgaccggatg gcggagaacg ccgatactcg cggcattgaa
     6121 cgcgtaaact atctggcact gctggcactc ggcactttct attttctctg cgctttcctg
     6181 cctatctact tcggcgcgga acatgcaaaa accatcattg atgtcctgcc gcaacgatta
     6241 atcgacggcc tcggcgtcgc gggcggcatc atgccagcaa tcggttttgc cgtgctgctg
     6301 aaaatcatga tgaaaaacgt ctacatcccc tacttcatcc tgggctttgt tgccgcagcc
     6361 tggctcaagt taccggtgct ggctattgct gccgccgcgc tggcgatggc gctgatcgat
     6421 ctgctgcgta aatctcctga accgactcaa cctgcggcac agaaagagga atttgaagat
     6481 ggcatctaat caaaccaccc tgccgaacgt cagtgaaaac gaagaaacat tgctgactgg
     6541 cgtcaatgaa aacgtgtatg aagatcagag cattggcgcg gagctgacga aaaaagatat
     6601 caatcgcgtc gcctggcgtt ccatgctgtt acaagcgtct tttaactacg aacgtatgca
     6661 ggcttccggc tggttgtacg gtctgctgcc tgctctgaaa aagatccaca ctaataaacg
     6721 cgacctggcg cgcgccatga aggggcatat gggtttcttc aatacccatc cgtttctggt
     6781 gacatttgtt atcggcatta tccttgcgat ggagcgttct aagcaggacg ttaacagtat
     6841 tcagagcacc aaaattgccg tcggtgcgcc gctcggcggg attggcgatg caatgttctg
     6901 gctaacgcta ctgccgattt gtggcgggat tggagccagt ctggcattgc aaggctccat
     6961 tcttggcgca gtcgtcttta ttgtgctgtt caacgtggtc cacctggggc tgcgttttgg
     7021 tctggcgcat tatgcttacc gcatgggcgt ggcggcgatt ccactcatta aagctaatac
     7081 caaaaaagtc ggccatgctg cgtctatcgt tgggatgacg gtaatcggtg cgctggtggc
     7141 aacctatgtt cgtttaagca ccacgctgga aatcacagcg ggcgacgcag tggttaagtt
     7201 acaggctgat gttatcgaca aactgatgcc tgccttctta ccgttggtct acaccctgac
     7261 catgttttgg ttggtacgcc gcggctggag tccgctgcgt ctgattgcag ttaccgtggt
     7321 tctcggcatc gttggtaaat tctgccattt cctttaaata caatgaggta ttagatgtta
     7381 agtattattt tgacagggca tggcgggttt gccagcggca tggaaaaagc aatgaagcaa
     7441 attctcggtg agcaatcgca gtttatcgcc atcgattttc cggaaacctc aagcaccgcg
     7501 ctgcttacat cgcagcttga agaggccatc gcccaactgg attgtgaaga cggcattgtt
     7561 tttctgacgg atttgctggg cggcacgccg tttcgcgtag cttcaacgct ggcgatgcaa
     7621 aaaccgggct gtgaagtgat caccggcacc aatttgcaac tgctactgga gatggtgctg
     7681 gagcgcgaag ggttaagcgg tgaagaattc cgtgtacagg cgctggagtg tggacatcgt
     7741 gggctgacca gtctggtgga cgagttaggc cgctgccatg aagaatgtcc ggtcgaggaa
     7801 gggatatgac acacgttctg cgcgccagaa ggctgctgac tgaagaggga tggctcgatg
     7861 accatcagtt gcgtattgct gacggtgtca tcgcagcaat cgaaccgatt ccagtgagcg
     7921 tgactgaacg cgatgcggaa ctgctctgcc ccgcttacat cgacacccat gtacacggtg
     7981 gtgcgggcgt tgatgtaatg gatgacgcgc cggatgtact cgacaagctg gcaatgcaca
     8041 aggcacgcga aggtgtcggc agttggttgc cgactaccgt aaccgcgccg cttagtacca
     8101 ttcatgcggc gctgaaacgt attgctcaac gttgccaacg cggcggacct ggtgcgcaag
     8161 tgctggggag ttatctcgaa ggaccgtact tcacgccgca gaataaaggc gcgcatccgc
     8221 cggagttgtt tcgcgagctt gaaattgccg agctggatca attgattgcc gtttctcagc
     8281 acaccttacg cgtggtagcg ctggcaccgg aaaaagaggg ggcattgcag gccatccgcc
     8341 atcttaaaca gcaaaatgta cgagtgatgc tggggcatag cgcggcgacc tggcaacaaa
     8401 ctcgcgccgc gtttgatgct ggtgccgacg gcctggtgca ttgctataac gggatgacag
     8461 gtttacatca ccgcgaaccg ggaatggttg gcgcgggatt aacggacaag cgcgcctggc
     8521 tggaactgat agccgatggt catcatgtgc atccggcggc aatgtcgctg tgttgttgct
     8581 gtgcgaaaga gagaatcgta ctgatcaccg acgcgatgca ggcagccggg atgccggatg
     8641 gtcgctatac gttatgtggc gaagaagtgc agatgcacgg tggcgttgtc cgtaccgcgt
     8701 ccggtgggct ggcgggcagt acgctgtctg ttgatgcggc agtgcgcaac atggtcgagt
     8761 tgacgggcgt aacgcctgcg gaagccattc atatggcgtc gctgcatccg gcgcgaatgc
     8821 tgggtgttga tggtgttctg ggatcgctta aaccgggcaa acgcgccagc gtcgttgcgc
     8881 tggatagcgg gctgcatgtg caacaaatct ggattcaggg tcaattagct tcgttttgat
     8941 ggtttgctcc attattgggc cttcacttcc cccgtaaggc ctttcttttt ctttcgtttt
     9001 gatctgtgca gcggtgtcgg atgcgacgct aacgcgtctt atccgaccta cagttggtga
     9061 ccgcaaggcc ggataaagcg tttgcgccgc atccagcaat cccttttgct tcctttatct
     9121 tttctttcaa cgatcacgga tttcgtttta tttccttttt ctccattgaa ctttcggttt
     9181 cttttctata gattttaatc aacgaaagac atcaccaagt gaaatgaaac gaaaggcaag
     9241 tgaaagcgac aacgcccgac gtcaagttca tcagactaag gattgagtta tgccagaaaa
     9301 ttacacccct gctgccgccg caaccggtac atggactgaa gaagagatcc gccatcagcc
     9361 tcgcgcatgg atccgttcgc tcaccaacat cgacgcgcta cattccgcgc ttaataactt
     9421 ccttgaaccg ttactgcgca aagagaatct gcggatcatc ctgaccggag ccggaacctc
     9481 ggcatttatc ggtgacatca tcgcgccgtg gctcgccagc cataccggta aaaacttcag
     9541 cgccgtaccg accaccgatc tggtcactaa tccgatggac tacctgaacc cagcccatcc
     9601 gctgctgttg atctcctttg gtcgatccgg caacagcccg gaaagcgtcg cagccgtgga
     9661 actggcaaat caatttgtac cggaatgcta tcacctgccg atcacctgca acgaagcggg
     9721 cgctctttac caaaacgcga tcaacagcga taacgcgttt gccgtgctga tgcccgcaga
     9781 aacgcacgat cgcggctttg cgatgaccag cagcattacc accatgatgg ccagctgcct
     9841 cgcggttttc gcacctgaga cgatcaacag ccaaaccttc cgcgacgtgg cggatcgttg
     9901 ccaggcgatc ctgacctcac tgggcgattt cagcgaaggt gtgtttggtt acgcaccgtg
     9961 gaaacggatc gtttatctcg gcagcggtgg cttacagggc gcagcacgcg agtcggcgct
    10021 gaaagtgctg gaactgacgg cgggtaaact ggcggccttt tatgattctc caaccggatt
    10081 ccgtcatgga ccaaaatcgc tggtcgataa cgaaacactg gtggtggtat ttgtctccag
    10141 ccacccttac acccgtcagt atgatcttga tctgctggct gaacttcacc gtgacaacca
    10201 ggcaatgcgt gtaatcgcca tcgccgcgga aagcagcgac atcgtcgctg ccggtccaca
    10261 tatcatcctg ccaccgtcac gtcactttat cgacgttgag caggcatttt gcttcctgat
    10321 gtacgcccag acgtttgcac tgatgcagtc gctgcacatg ggcaatacgc cggatacccc
    10381 atcagccagt ggcaccgtta accgcgtggt gcaaggcgta atcattcatc cgtggcaggc
    10441 ataagaggat cgcattatga gcattatctc caccaaatat ctgttacagg acgcccaggc
    10501 caatggctac gcggtgcctg cttttaacat tcataacgcc gagacgatcc aggcgatcct
    10561 cgaagtgtgc agtgaaatgc gatcgccggt gatcctcgcc ggaacgccgg ggacctttaa
    10621 acatatcgcg ctggaagaga tctacgccct gtgcagcgcc tattccacaa cctacaacat
    10681 gccactggcg ctgcatctcg accaccacga atcgctggat gatattcgcc gtaaagtcca
    10741 cgcaggtgtg cgcagtgcga tgatcgacgg cagccacttc ccgtttgccg agaacgtaaa
    10801 gctggtgaaa tcggttgtcg acttctgcca ctcccaggat tgcagcgtgg aagcagaact
    10861 gggccgcctg ggcggtgttg aagatgacat gagcgttgac gccgaaagtg cattcctgac
    10921 cgatccacaa gaagctaaac gctttgtcga actgactggc gtcgacagct tggcggtagc
    10981 gattggtacg gcgcacggct tatacagcaa aacgccgaag attgatttcc agcggctggc
    11041 ggaaattcgt gaagtggtgg atgttcctct ggtgctacat ggtgccagcg atgttccgga
    11101 tgaatttgtc cgtcgcacta ttgaacttgg cgtcacaaaa gtgaacgttg ccacagaatt
    11161 aaaaatagcc ttcgccggcg cggttaaagc ctggtttgcg gaaaatccgc agggtaatga
    11221 tcctcgttat tatatgcgcg tcggaatgga tgcgatgaaa gaagttgtca gaaataaaat
    11281 taatgtctgt ggttcagcga atcgaatttc agcataatca ttagtttttt atgatttatc
    11341 ccaatgtact tcccgattaa tatcggggag tgccttaatg gaaaaggaga tgactaaacc
    11401 tta
//
LOCUS       DAZKIF010000134        11240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZKIF010000134 DAZKIF010000000
VERSION     DAZKIF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.135"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            54..959
                     /locus_tag="ACHOTW_003859"
     CDS             54..959
                     /locus_tag="ACHOTW_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9845882.1"
                     /translation="MRGKIAKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDTGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(993..1595)
                     /gene="cobO"
                     /locus_tag="ACHOTW_003860"
     CDS             complement(993..1595)
                     /gene="cobO"
                     /locus_tag="ACHOTW_003860"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP9845883.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1605..3257)
                     /locus_tag="ACHOTW_003861"
     CDS             complement(1605..3257)
                     /locus_tag="ACHOTW_003861"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9845884.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3365..4645)
                     /locus_tag="ACHOTW_003862"
     CDS             complement(3365..4645)
                     /locus_tag="ACHOTW_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFP9845885.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4806..5123)
                     /locus_tag="ACHOTW_003863"
     CDS             complement(4806..5123)
                     /locus_tag="ACHOTW_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFP9845886.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5120..6490)
                     /locus_tag="ACHOTW_003864"
     CDS             complement(5120..6490)
                     /locus_tag="ACHOTW_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFP9845887.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6494..7735)
                     /locus_tag="ACHOTW_003865"
     CDS             complement(6494..7735)
                     /locus_tag="ACHOTW_003865"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFP9845888.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7735..9180)
                     /locus_tag="ACHOTW_003866"
     CDS             complement(7735..9180)
                     /locus_tag="ACHOTW_003866"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFP9845889.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9199..10587)
                     /gene="glmL"
                     /locus_tag="ACHOTW_003867"
     CDS             complement(9199..10587)
                     /gene="glmL"
                     /locus_tag="ACHOTW_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFP9845890.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10587..11036)
                     /gene="glmS"
                     /locus_tag="ACHOTW_003868"
     CDS             complement(10587..11036)
                     /gene="glmS"
                     /locus_tag="ACHOTW_003868"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFP9845891.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         2667 a         2819 c         2829 g         2925 t
ORIGIN      
        1 cgtaataagt aatgtatttt tgatggttta aactattttt aagggacggc attatgcgag
       61 ggaaaatagc taaaacggaa ctgctggtga cttttgaggt cgtcgcacgt catgaaagtt
      121 ataccagagc ggcagaggag ttagcgttaa cccaaagtgc agtattcaga caagtaagtg
      181 cgctggagga atttttacgt actccgttat tttcacactc taaaaaacgc atttttctta
      241 atgatacagg gaagtattac ctggggatag taaaagaaac attaaataag ctggaacgcg
      301 ataccaatac cattatgacc tggcagccta cggttcaggt tattgaactt gccgttaatc
      361 ctacttttag tacccactgg ttaatcccaa atttacatga attcacaaag ttacatcccg
      421 atattattgt taatattcat tctttagcta ataacggcga ttttcttaat cgggagtacg
      481 atgctgtcat tatgcgcgaa aatttttgcg cgccctgggc ggaagtagaa tatctctttg
      541 aagaggagat tttgccggta tgcagcggca gtttgcttgc aatgtccgat caaaaactgt
      601 cagtggcaga actgcttact gagttaccac ttctgcatca aagcacccgt attactggct
      661 gggaagagtg gtttgcttta tctggcgtta gcagtccgct ggtcaacaac ggaccacgtt
      721 tcgatttgct ttcaatgctg attgccgccg tgcgctctaa tctcggcgtg gcgctgttac
      781 cacgtttcgc tattcaacat gatttggata gtggcgatat ggtgattccc tgtgatgtgc
      841 ctattcgtac cggtaatcgt tttattatga cgtggcagga agagaagtct gactcaccgc
      901 acttgcagca atttcgtgaa tggttgctgg cgaaatcggt cgtaccgcag gagatgtaaa
      961 acctgcgcct ggcgggatgc caggcgcagt tattaccagt cgatacccgg ctgggcctga
     1021 atgccgttat caaaagcatg tttgacgggg cggatttcgc tgaccgtatc tgccattttt
     1081 aatatctgac tgtggcaacc gcgtccggtg acaatgacac tttgttgtgc cgggcggttt
     1141 tgcagagaag cgatcacttc ttcggtatcc agataatggt aagccagcat ataggtcagc
     1201 tcatcaagca ccactaagtc gtaacgcttg tcagccagca tccgtttgct ttccgaccag
     1261 acctctttcg ccgcgtcgat atccgcttgc cggttctgtg tctcccaggt aaaaccggtt
     1321 cccataatat gaaactcaac ccccagcggc tggagaaggt tgtattcgcc gttgtcccat
     1381 tgccccttga tgtactgcgc aacccccacc gttttgccat gacccaccgc gcgcgtcaca
     1441 gtaccaaacg cggcagtgga tttccctttg ccgttgccgg tgaaaacaat caagatccct
     1501 tttttctcta tcgccgccgc gacgcgcgta tcgacctgtt ccttcaattt ctgttggcgc
     1561 tgcacatgcc gttcagttga aattcgcgct tccattcatt ttccttaatg tcccgcaggg
     1621 caactggcgc agcggtgttg ctgctcaaac tgactaaaga aattattgcc tttatcatct
     1681 accaggatga atgcgggcag attttccact tccatcatcc acaccgcttc catacccagc
     1741 tccgggtatt ccaggcaacg cagacttttg acatattctt gcgccagtaa cgcagctgca
     1801 ccgccaatac ttcccagatt gaagccgcca tgtttgtgac aggcatcggt gacctgctgg
     1861 ctgcggttac ctttcgataa catcactaga ctgccgccag ccgcctggaa ggtatcgata
     1921 taaccgtcca ttcgaccgcc cgtggttgga cccagtgaac cgcaggccat gttctccggt
     1981 gttttcgccg ggccagcgta atagacaata tgatgcttca ggtactccgg catcggttcg
     2041 ccgctatcca gccgcgcttt tattttggcg tgggcgatat cgcgcgccac tacgatggga
     2101 ccacttaacg acacgcgggt gcctactggt aatctggcga gatcctgcat cacgtcacgc
     2161 aacggacggt ttagatccag ttgtacatgt tgcgcgtgat tttcttcccg cagtgaggct
     2221 ggaatatatt gccccggatt gtgttcgagt ttttccagcc agataccgtg tttgttaatt
     2281 tttgctttga tattgcgatc ggcagaacag gagagcgcca tagctatcgg gcaagagcca
     2341 ccgtgacgcg gcagacgaat gacgcgaata tcatgagcga aatatttacc cccaaattgc
     2401 gcaccaatac caaattgctg actggcctcg agcaacactt tttccagctc aatatcacgg
     2461 aatgcttgtc cctgctcgtt tccgctggtg ggcaaattgt cgtaatattt cgttgaggcc
     2521 agcttggcaa tttttaaggt ttgatcggct gaaagccctc cgacgacaaa ggcgatgtgg
     2581 taaggcgggc aggcagcggt gcccagcgat ttcatttttt cgattaaaaa tgcggtcagt
     2641 ttttctggtt gcaataagga tttggtttcc tgataaagcg cggctttatt ggcggagccg
     2701 ccccctttat taacacagag aaaatggtac tcatcaccag cgacagcgct gatatcaatc
     2761 tgcgccggga gattggtttg cgtattgact tcggtataca tatccagcgg cgcattttgt
     2821 gaaaagcgca gattattttc ctggaaagta ctgtaaatgc ctttacttaa ggcttcggcg
     2881 tcgttacctc cggtccatat ctgttgccct ttactggcaa caatagttgc ggtgccggta
     2941 tcctgacagt tgggtaatac cccttttgct gagacttcag cattacgtaa taactgcaac
     3001 gcaacgtatt tatcattact gctggcctgc tgatcgttaa gaatgctggc gacctgttgc
     3061 aaatgagcgg aacgtaagaa aaaagaggct tcataaaatg cctgccgggc aagcagcgtt
     3121 aatgcttcag gagcgatttt tatcacttct tcgccgtcta attccgttac tgtaatatgt
     3181 tgatcgctaa ttaatgtata ttctgtaccg tctttatttt gtagaaacgg ttcttgccag
     3241 ataaatggtt tggacatatt tcacttttca ccttaaacgt tagtgttact taattgccgt
     3301 ctaacttcat gatccggctt ataaaaatgc gtagtcattg gcttctgatt gcttaagatc
     3361 cccctcaggg cagagcaacg ttcgattcat tgggttttaa tgctttaccc cgacagccct
     3421 cgccgaaatg ttcgccttcc caccgactca ccatcgcagc gcccattgcg ttgctcatga
     3481 cgtttgtcgc ggaacgcccc atatcaagga acggatcaac gcccatcagc aagatcagcc
     3541 ccgcttccgg aatattgaac tggttgagcg tggcggcgat aaccaccatt gaggcgcgtg
     3601 gtacgccagc cattcctttc gaggtcaaca tcaggatcaa cagcatggtg atttgctcac
     3661 cgatggataa atggatattg caggcctggg cgatgaaaac tgtggcgaag gagcagtagg
     3721 ccattgatcc aacgagatta aatgagtagc caatgggtaa gacaaagctg gcaattttgg
     3781 gggaaacgcc aaattgctcc agtttttcaa gcgttcccgg aaaagccgct tcagaactgg
     3841 atgtggtaaa tgccagcagg gcgggttccg aaagggcgcg ggtcaggcgt ctgatgcagg
     3901 ggccgacata aacgatggcc agaccgataa gcagtaccca aagtaataac atggtgaaat
     3961 aaaattcacc cataaagatc ccggcgctca ccataactgc cagtcctcgt tcagcaatca
     4021 atgctgaaat agcagcgaat acggtcaggg gagcgaagag catgacgtag ccagtgagct
     4081 ttaacatggc atgtgccagc gaatctaagg cgtgaacgat ggcgctgcct ttctcgccaa
     4141 tcgccgtcag gctacagccg aggaaaattg agaacaccac gatttgcaaa atttcattgt
     4201 gcgccatggc atcgacaatg ctggtgggga aagcatgcga aataaatact tttagcgtaa
     4261 agggttctgc cgcgaccact ccggtggttt cggcaccgtg tgcaacaaaa ttaattcctg
     4321 tacctggcat gaagaaattt accgttatca agcctaaggc aattgacagc aatgaggcgc
     4381 aaataaataa aaagagtgtt ttagaaaaaa tacgaccaag ggctttggca tcgcccattt
     4441 tagcaatacc taccaccagg gtagagacca ctaacggagc gataatcatt tttatcagtc
     4501 gtaagaaaat cgtcgtgaat atcgatattt cttgcgcata gagttttgcg gtttccggtg
     4561 aagcagtgtt attgagcact acgccgataa tcattccaag taccagcgcc aaaagaatca
     4621 tcgtggttaa acttattttc ttcatttttt cctccatcag gtgcacggcg ataaatcaga
     4681 atcaggagaa gtagatgcct gaatagagat tcaggcatct acctgctatt acaaccttac
     4741 aaatagctga agtgttgaac tgcaataagt gacgggatgt cagttggcta aaagtattcg
     4801 gatttttaca aatcgaaact catatttaat aaaggtgcat gttgctgtgc accatagaca
     4861 tcattgtcac ctacattccc ggagataata tctcgtggca tgacgatttt tactgcgtta
     4921 gaaggatcaa accagacaat acgatgaata aagtcaggtt caacatgata taagcgtgca
     4981 atcagttctg gggttaattg ccctgatgat ttaactcgct catagttttc tttcgtttta
     5041 aataaaatat ctaaaaccag ttcgtaaggt ccggcatttt tcgaacgaat gacttgcgcc
     5101 agtgagacaa ttgactgttt catgcctgaa ctccttctgg cgtcacctgt tcaatatgga
     5161 aatcaaaacg cagagcgtcg ttggcttcaa tcaggtgata tatggaaaat tcgtataccg
     5221 gcccgccctg gatatcagaa ggggagaacg ggaacgccag attacctgcg gtagcgatgc
     5281 ggttttcata gccgtagtgc agcatggtag agcgcaccag cgagcaaacg ctgttggcaa
     5341 tttcctgggt cggtgcaact acatcaagga caatccccag ctcatgcccg gcagtttgca
     5401 tcggttcatg gtctcccatc accccgtttt tgccgtacag gtggaaattg atgcgaatgc
     5461 tgtcatcgtc gagcgacagg ttacgcgaaa cgctggtttt gacttcatcg atgattttat
     5521 cgatcccggc gatcataatc gggtcgcgcg ttccggcgat ggtcagacag cggaagccca
     5581 cccgtcgtgc accttccagt ttcagggcat acggcgtttc ttcatgctta gaaccgctga
     5641 cgtagacttc gccgtcattt actgctttaa atgtgcagcc tttcaggttc aacacgccgc
     5701 caggtcctgg caggaagtag ggatcggatt tctcatacag tgtgtgtgca gctgctgacg
     5761 tttcggtaaa tttacgcttc ggattaaatg ttttcagcgt aaagccgttg tcatcaatga
     5821 tgcccatcgc acagtcagag cctgagccgg gcgttgcggc gattgccgcg cattcaagga
     5881 ttttcccaca atgcagcgcc agaccttcat caaagccttg catgatcggg agcgcagcaa
     5941 agcaggccgg gtcgtaagcg cgcccgccca gcaccacttg cgcgcgttct ttcagcgccc
     6001 gctggaaggg ttcgatgccc atttgcgcga cgatatacgt actttcgtca atcgcgtcgt
     6061 gggttaacgg cgggacaaaa tccagcgccg tgattttgcc gttatccagc gcctggtgga
     6121 cgatggcttt gtcaacgtct gccgggatca acgccatcga gaacgagagg tgctcttccc
     6181 gggcaatctc atgaattatt tctcggcacc actccaggtg tggtgcggca ccagaaccgc
     6241 cagccgttcc gatcaccacc ggtatattat tttgcacgcc tgcggtgatc atatagcgca
     6301 gatcgcgctt gacccctgcg cgatcggtaa acggtttgcc tgccccgagg tagtgggggc
     6361 ctggatcgga agagcctgca tcaacggcga tcagatcggg cgattctgcc atcgctttgc
     6421 ggaagctctc ttccgggaat ccatacccga ggattgccgt tggcgataag actttaaaag
     6481 aacgagccat ttgttaatcc ttagcctgca acagcgcgat agtacgattc atttcgttga
     6541 agacgatatc gaggccttcg tcaaagccca tccctggttt taccagcata cgcatggggc
     6601 gagcggcaag ggcgacgtgg acacaggtgc gggcactgac atcagtttca ttgcaggtgc
     6661 cgccctggta cgcttccatg ctgtggctgt tgcagtaaag aaccgcatcg acgatgttgt
     6721 gaatactgcc cagatccggg gttttgattt gcaccatgtg acaactggca gcatcagtga
     6781 aatcaacaat atcctggtag gtgttacacc attcatcggc cacaattttc acgccggaac
     6841 cgaggcgcgt cagctctttg gtaatcgcgg tcagcaggcg aatttgatcg ggcttgttac
     6901 cggcatcgac cggcccttcg atgtagagcg gcaggccttg cgcttctttt tccaggctgg
     6961 cgatgtattg cgcacagcga agcggatcca tatcgaagat cagaccgata gtgccgtata
     7021 catcgatgtg cagggtaggg tggtagcgtg cgctggtgcg cttgcttaga atgcgatccg
     7081 acaaccagcg gacatattcg cgcagttttt cacctttaaa gcccagcttc tcttcgacgt
     7141 tattaatcag cgcatggggc agcacgtcga tgcctttaag gatcatctta tcgacggcga
     7201 tatatcgatc gtcgccgctc tgaccaaata atggaatgga ttccgccacg cgtggcaact
     7261 gccattcatc acagaccact tcagttttca gacggccggt tgccagcgcg gtagcatcaa
     7321 gcagcgcctg tgataatccg tagcgcacgg cggtatgcag caagttgccg tcaatacgca
     7381 atttgtcgaa gaaacgggca ttcggcagga aagcatccac atcgcggcct accagtaatg
     7441 gcttgatatg gtcgttgagg aacggaataa agtgctctgc gaggaacagt ggatcgcgac
     7501 cgcctgcccc ggaatactgt acggcagtac agtcacctac ggcgaccgcg ccgttttcca
     7561 gaatcaactg taccgatacg cactccccgg cctgacgtac ggcgttaaac ccctgtgtta
     7621 ctggctcccc ggtataaaaa aagccgtcat gacccgctcc gttttttatc gcctgctggt
     7681 catcgaaata gaatgaggag tagccagcgg tgaacagagc ctgttttatt ttcattatgg
     7741 tcttcctatt aatttactgt gggatacagc attgatgtca tctacaacca tctggaatgt
     7801 cggttggcgt ccttcacagc gtgcgcgttc cgcaacgtaa tcgtggtgga ggtcgagaat
     7861 atcttttggc aatggaacgg ctccggcttc cagtacgcgt atcgcgccgg tgttgtcacg
     7921 gaccggaaga attttgcccg cgttgcaggc ggctggcgca aacggcacat ccagtacgcc
     7981 cgcttcaaat gccagcaccg ttccgcgcgc aatatcacca ttacccagtt cgaacacttt
     8041 attcagtacc gcgcgaacct cgctcttaat cagttcaatt tccagttcaa ccgcagggca
     8101 cggcgggaat ttctgctcat tgaccatgtt gagcatttgg cgggaggctt tcagcccctg
     8161 aatgttggca gcggcagtcg ggatacccca ggcttcgtgc ggacttttgg tgatgacttt
     8221 ggtggcacca gacatccccg cgacggctgc gccccaggag ataatagcga acgctttcga
     8281 ttcgtcttcc ggaaagccgc ccatccactg gtgaaacacg gtactcaatt catagtcggt
     8341 atagccatag ctctgaaaat attcgtgtgc aagttcacgc agagactgga tcgccgcaac
     8401 atcctgcgtc aggctgccga cctggccata accaacggtg atagatttca caccctgttc
     8461 cagcgccagc aagccttcga ttatcgcgat agagtgcgag ataaacggcg ggatcagcgt
     8521 acccgttagt gggccaaacg gttcgcggtt gatgcggatc ccgtgttctt catacatccc
     8581 cattaaacgg tcgcagtact gccagtcgcg gatcgatttt tccagcgtta cgcgtttggc
     8641 atacggaatg ttgtaggaaa tgccaccgcc ttcataactg gtaaagccgc tggccattga
     8701 gatttcagcc agcagacgcg catctggcgt gccgtggcgg atctgtagcg gtttttgcag
     8761 ggtttcggtg aggcgacggc aagctgcgac accatggtta actaccggta aaccgttcag
     8821 tttagaggtt ccggcttcga tggatttttt aatccctaca gccgcttctt cgtaccgatt
     8881 cagacgggta tatgcatcga tggtgcttgg cagcagatcg cactcttctt gtagtgtttt
     8941 cagcagttcg atgtgttcgt ccatcagcgc cacacctgcg cgcggttggc taagggtttt
     9001 accttccttg tctgctttca gcagcgcaag agagaaacgt ttatgctcag ggatggtttg
     9061 ttggtattta acgccatctt caaagttttc aacatccttg ccggtttccc aggttttcag
     9121 tacctgttgc cgctcggtca tgaattcgtc atgggttaat ttcttatttc gaagttccat
     9181 tgattgcagc cttaattgtt atagggttaa gcattgaata ctggtgcgcg cggcggccag
     9241 cggatcgact ctggcgacgt ttgccagcag tggtaatagc ccccgggaat cgcggtaata
     9301 ttcaaattcg gtcggtaaga gaatgcgttt accgtcgtcg tttaactcgc ggtatttcaa
     9361 ccagtgatgc atatcgaact gactggcacg cgagagccat ccgccggatc cgacgacttt
     9421 acgcacggtt gtcaggtcac gtcccatctg taaatcgaca ttgccgacgc aggtgcagac
     9481 ctcttttttg gtgcccgcat gtctttcggt ggcataaccc acacacagtc cggccaggac
     9541 ggtatcgaaa tatttctctt ctgcatttgc gggtaagtag tcgggatgag cgactagatg
     9601 acggaggtaa tgataaaaag cgtccagctg tgcgggttgc tgagcaaaat tcacattgac
     9661 taactctttc gcactttcac caaccaccac tgccgagacg cgcattccca gatcgccttc
     9721 caccgtccgt tttacgaagg gttccggtac gccgtgtaaa acggtgtcgg gcgagagcgt
     9781 gttggcacag gcggaataaa catcggtggt tgcgccgccc atgtcgatca gcatgaactc
     9841 tttccagctg tgatcgacgt tactgattgc cttgaccagt tcaaagaccg tccatggtgt
     9901 cggcattggt tcttcgcctg ttttatcaac aatgacatcc agccctttcc ctttaacgat
     9961 gcgtttaagg aatatgtcgc aaatagcctg gcgtgcagct acagggttcg gatggtcaag
    10021 atcgggcaga acgttgtcga caatggtcag gtctttatgc ccgaggatct cctgcacctc
    10081 gtcctgaata tcccggtttc cggcataaat aatggcgcag tcgagtttgg attctgccag
    10141 cacacgggcg ttattcagtc cgtagctttc ttctccgccg tcggtgccgc cggtgaacaa
    10201 gagaatatcg ggctgcgttt cttccagtgc ctggatatca cggcggttca gcttgtaggc
    10261 gtaatattgc gcaattttag cacccgcaga gtgagcggtt actttcgcgg tttccagggt
    10321 gatagaaggc accaatccca ttgcggcaac cgccagccca cctttggcag aggaggaata
    10381 cttcaatgca acctcaccgc tgtttaacag ggggagggca ttatctacgt ttaacacctg
    10441 gttcaggctg gagaaaaacc ccttcgccag atgatgggtg gtggttgggg tcagtacgtg
    10501 gttaaccaac gttaacgcgt ccccctcccg ggtaaagagg gctgctttgg tccacgtcga
    10561 tccaatatcg acagagacga tttgcatcag atagcctctt caagaatacg agtatcaacg
    10621 tcatgacgtt ggttgatatc gtgggccatc agctggcaaa catcttctaa gtcatgactt
    10681 ggggcaaaga cgcgatcaaa tcccatctct ttgaatttgg tttccacgtc agcgaagtca
    10741 tgttttccga ccaccaggtt accgcccaca tagagcagga tgtcccccag tccacgctcg
    10801 atgcagcgtt cgcgcatgcc cagacagtcg atatcgccat gcccatagat agaggagacg
    10861 acaatcgcgt cagcgccagt ttcaatggca gcatcaatat attcgtcctg gctgaccatt
    10921 acgcccagat taatgactct gaagtcgtgg ttgctaaaaa cgcggtccag aactttattg
    10981 cctactgcat ggcagtccgc gccaataacg ccaatcacaa gtgttgcttt cttcatgggt
    11041 aatcctttgt aagacagaga gtaaaataag ttacacacgt cgtatttatg gccgttcata
    11101 acggcacagg tttaatatca caggagtgtc tttatctgac cgtaatgttg caattgatct
    11161 attttcaaaa tgtctttcgg gaaatgattg tttgaagtgt gtttttaaat ttccatgtta
    11221 attcagtgtg ttatgtcaac
//
LOCUS       DAZKIF010000135        11071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZKIF010000135 DAZKIF010000000
VERSION     DAZKIF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.136"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            442..1782
                     /gene="dcuB"
                     /locus_tag="ACHOTW_003869"
     CDS             442..1782
                     /gene="dcuB"
                     /locus_tag="ACHOTW_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP9845892.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1860..3506
                     /gene="fumB"
                     /locus_tag="ACHOTW_003870"
     CDS             1860..3506
                     /gene="fumB"
                     /locus_tag="ACHOTW_003870"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9845893.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEKAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            3629..4258
                     /gene="yjdF"
                     /locus_tag="ACHOTW_003871"
     CDS             3629..4258
                     /gene="yjdF"
                     /locus_tag="ACHOTW_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP9845894.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4397..5818)
                     /gene="melB"
                     /locus_tag="ACHOTW_003872"
     CDS             complement(4397..5818)
                     /gene="melB"
                     /locus_tag="ACHOTW_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFP9845895.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(5921..7276)
                     /gene="melA"
                     /locus_tag="ACHOTW_003873"
     CDS             complement(5921..7276)
                     /gene="melA"
                     /locus_tag="ACHOTW_003873"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9845896.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7559..8467
                     /gene="melR"
                     /locus_tag="ACHOTW_003874"
     CDS             7559..8467
                     /gene="melR"
                     /locus_tag="ACHOTW_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP9845897.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            8666..10933
                     /gene="adiA"
                     /locus_tag="ACHOTW_003875"
     CDS             8666..10933
                     /gene="adiA"
                     /locus_tag="ACHOTW_003875"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP9845898.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2847 a         2928 c         2696 g         2600 t
ORIGIN      
        1 taatatctct tcaggtgaac ggtgttttta atttcaaaac gctaacaaaa gttaattaac
       61 tattatgtca cccgcattat gtgtattttt acccacaaat gggtagatca gattaatcta
      121 taaacctaat gatatctgcc ctgagaacaa aaaatagacc gataaatatc aataagataa
      181 cagcaaacaa aacattaaca tctgcgcagt acaaacttta aacccatcgc cagagagttt
      241 ttctctctga aaaagccgct tatcacagtg cataaatttg ccgctgcttt aatcagccaa
      301 tattcactgt gaggtatttg ctaaagccgg taacgaccaa acggatattt agtcaggctc
      361 tgaaaacagt tcatacaaaa cagaacgtga ctgtgatcta ttcagcaaaa atttaaatag
      421 gattatcgcg agggttcaca catgttattt actatccaac ttatcataat actgatatgt
      481 ctgttttatg gtgccagaaa gggtggtatc gcgctgggtt tattaggcgg tatcggtctg
      541 gtcattctgg tcttcgtctt ccaccttcag ccaggtaaac caccagttga tgtcatgctg
      601 gttatcattg cggtggtggc ggcatcggcg accttgcaag cttcgggcgg tcttgatgtc
      661 atgctgcaaa ttgccgagaa gctgctgcgc cgcaacccga aatatgtctc aattgtcgcg
      721 ccgtttgtga cctgtacgct gaccattctt tgcggtacgg gtcatgtggt ttacaccatt
      781 ctgccgatca tctacgacgt cgccattaag aacaacatcc gtccggaacg tccgatggcg
      841 gcaagttcta tcggtgcaca gatggggatt atcgccagtc cggtgtcggt tgcggtcgtg
      901 tctctggttg caatgctggg taatgtcacc tttgatggtc gccatcttga gttcctcgac
      961 ctgctggcaa tcaccattcc atcgacgtta atcggtatcc tggcgatcgg tatcttcagc
     1021 tggttccgcg gtaaagatct ggataaagac gaagagttcc agaaattcat ctccgtaccg
     1081 gaaaaccgtg agtatgttta cggtgatacc gcgacgctgc tggataaaaa actgccgaaa
     1141 agcaactggc tggcaatgtg gattttcctc ggggcaatcg ctgtagtcgc acttcttggt
     1201 gctgattcgg acctgcgtcc atccttcggc ggcaaaccgc tgtcgatggt actggttatt
     1261 cagatgttta tgctgctgac cggggcgctg attattatcc tgaccaaaac caatcccgcg
     1321 tctatctcaa aaaacgaagt cttccgttcc ggtatgatcg ccatcgtggc ggtgtacggt
     1381 atcgcatgga tggcagaaac catgttcggt gcgcatatgt ctgaaattca gggcgtactg
     1441 ggtgaaatgg tgaaagagta tccgtgggcc tatgccattg ttctgctgct ggtttccaag
     1501 tttgtaaact ctcaggctgc ggcgctggcg gcgattgttc cggtcgcgct agcgatcggc
     1561 gttgatccgg catacatcgt ggcttcagca ccggcttgct acggttatta catcctgccg
     1621 acttatccga gcgatctggc agcgattcag tttgaccgtt ccggcaccac ccacatcggt
     1681 cgcttcgtca tcaaccacag ctttattctg ccggggttga ttggtgtgag cgtatcgtgc
     1741 gtcttcggct ggatcttcgc cgcgatgtac gggttcttat aaatgcactt tgcgtgccgc
     1801 ccgttactac gcggcacgcc attttcgaat aacaaataca gagttacagg ctggaagcta
     1861 tgtcaaacaa accctttatc taccaggcac ctttcccgat ggggaaagac aataccgaat
     1921 actatctact cacttccgat tacgttagcg ttgccgactt cgacggcgaa accatcctga
     1981 aagtggaacc agaagccctg accctgctgg cgcagcaagc cttccacgac gcttctttca
     2041 tgctccgtcc ggcacaccag aaacaggttg cggctattct tcacgatcca gaagccagcg
     2101 aaaacgacaa gtacgtggca ctgcaattct taagaaactc cgaaatcgcc gccaaaggcg
     2161 tgctgccgac ctgccaggat accggcaccg cgatcatcgt cggtaaaaaa ggccagcgcg
     2221 tgtggaccgg tggcggtgat gaagaagcgc tgtcgaaagg cgtctataac acctatatcg
     2281 aagataacct gcgctactcg caaaacgcgg cgctggacat gtacaaagag gtcaacaccg
     2341 gcactaacct gcctgcgcaa atcgacctgt acgcggtaga tggcgatgag tacaaattcc
     2401 tttgcgttgc gaaaggcggc ggctctgcca acaaaaccta tctctaccag gaaaccaaag
     2461 ccctgctgac tcccggcaaa ctgaaaaact tcctcgtcga gaaaatgcgt accctcggta
     2521 ctgcagcctg cccgccgtac catatcgcgt ttgtgattgg cggtacgtct gtggaaacca
     2581 acctgaaaac cgttaagtta gcaagcgctc actattacga tgaactgccg acggaaggga
     2641 acgaacatgg tcaggcgttc cgcgatgtcc agctggaaca ggaactgctg gaaaaggccc
     2701 agaaactcgg tcttggcgcg cagtttggcg gtaaatactt cgcgcacgac attcgcgtta
     2761 tccgtctgcc acgtcacggc gcatcctgcc cggtaggcat gggcgtctcc tgctccgctg
     2821 accgtaacat taaagcgaaa atcaaccgcg aaggtatctg gatcgaaaaa ctggaacacc
     2881 atccaggtca gtacattcca caagaactgc gccaggccgg tgaaggcgaa gcggtgaagg
     2941 tcgaccttaa ccgcccgatg aaagagatcc tcgcccagct ttcgcaatat ccggtttcca
     3001 ctcgtttgtc gctcaccggc accattatcg tgggccgaga tattgcacac gccaagctga
     3061 aagagctgat tgacgccggt aaagaacttc cgcagtacat caaagatcac ccgatctact
     3121 acgcgggtcc ggcgaaaacc cctgccggtt atccatcagg ctcacttggc ccaaccaccg
     3181 caggtcgtat ggactcctat gtggatctgc tgcaatccca cggcggcagc atgatcatgc
     3241 tagcgaaagg taaccgcagt cagcaggtta ccgacgcgtg tcataaacac ggcggcttct
     3301 acctcggtag catcggcggt ccggcggcgg tactggcgca gcagagcatc aagcatctgg
     3361 aatgcgtcgc ttatccggag ctgggtatgg aagctatctg gaaaatcgaa gtagaagatt
     3421 tcccggcgtt tatcctggtc gatgacaaag gtaacgactt cttccagcaa atcatcaaca
     3481 aacagtgcgc gaactgcact aagtaacctc ttcggcccag cgcctggcag catgctgcca
     3541 ggtgatcccc ctgggccacc tcttttgcga ttgtaatttc acgcttgctg gtgaatagtc
     3601 agtattttcc ctgacttgcg aactcaccat gacccgcaca ctcaagccgt taattcttaa
     3661 caccggcgca ctggcgctaa cgttaatcct gatttatacc ggcatttcgg cccatgacaa
     3721 actcacctgg ctgatggaag tgacaccggt gattattgtc gtgccgctac tgcttgccac
     3781 cgctaaacgt tatccgttaa cgccgctgct ctatacgctc attttctttc acgccatcat
     3841 cctgatggtc ggcgggcaat acacctacgc gaaagtcccc atcggttttg aggtgcagga
     3901 atggctgggg ttgagccgta atccgtatga caagctgggg cattttttcc aggggctggt
     3961 gcctgcactg gtggcacgag agatccttgt gcgcgggatg tacgttcgcg gacgtaaaat
     4021 ggtggcgttt ctggtctgtt gcgtggcgct ggcgataagc gcgatgtatg aattaatcga
     4081 gtggtgggcg gcgctggcaa tgggccaggg agcggatgat tttctcggca cccagggcga
     4141 ccagtgggac acgcaatccg atatgttctg cgcgctgctt ggcgcattaa ccacggtgat
     4201 attcctcgct cgctttcatt gccgtcagct acggcgcttt ggcttgataa ccggataaca
     4261 acgtgcactt tgttgatgcc ggatgcggcg tgaattcctt atccggtcta caaaatttgc
     4321 aaatccaaag cattccatta agacgtggcc tgataaacat atcgcatcag gccattttcg
     4381 ttagctattg tccacgtcag gctttcacat cactcactgc accgaccgga atatcagcat
     4441 gaacaggctc gggcggtatt ttgcgatatt tatccagcag atgaatctgg atcctgcgca
     4501 gcgtgtcgcc attgaggcga tagaaacgga agtagagaat cagcgttacc atgaaaaaca
     4561 gagtcggtag agcaatcata ataaactgca tacctaatag ggcttgcgta gactgttcaa
     4621 cattcggtac atagccaatc atccctaaca caaccgcaat gaaaaaagcc gcaaaggctg
     4681 aaccgccctt caccaccata gtctgcacgg agtaagcgat gctttcacag cgtacgtgca
     4741 gtttatattc accgtaatca acggtatctg ccaccatgat gacctgtaat acccagaaaa
     4801 gcgccgttcc cacattcagc aaaatacccg caatcacaat gaggacgacg ttgtgatagc
     4861 tcattaatgc cattaacagg agaacaccac agcttaacac cggaagaata gatgctccgg
     4921 cccataaaat gcgtcgggat aatgatttaa ctaagcgtgg gaagaatact aacgtcacca
     4981 ggttagcagc tcccgcatac gacagataat aggggaacaa atccgcatca ccgataacat
     5041 atgagaaata atagatagca aagccggtaa taatgttgct ggctacatta taagcaagag
     5101 ccatacccaa gaggcatgaa agctgatcgt ttttataaat aagcgcaacg atggctttaa
     5161 gtgtcagatg gcttccttca gcagacggtt gattgtctga cgaaaagact tcatgcacat
     5221 tgcgcagagt gatgatggtt gaaacaataa aaaaggcgat cagtaccagt gtgaacatct
     5281 gaaagccaaa tccccgatcg ccaccgccga cataattaac aaatggtagc gtcacacctg
     5341 ccgtaacaaa gcccgccaga ctggcaaaaa aacgcggata aggaaccagt tgttcacgct
     5401 cacgtttatc gagcgtgatg gttggaacca gcgaccagaa gggaatatcc ataatggtgt
     5461 aagtcatgcc ccagaggatg taggtcacgc aaacaaaggc aatctgagta gtaccttcaa
     5521 acagatgcgc actaaagagg agaaataaga ttacagagtt tgccaacgta ccgatcagga
     5581 tccagggttt gaacttaccc catcgcgatc gcgtagcatt tacaatccat cccataatcg
     5641 gatcgttaat agcatcccag atcctcgcca ccagaaacaa agtaccgacc aaacccacag
     5701 acagcccgac gacatcggtg tagtaataca tgaggtacat atacacaatg ccgatcgcaa
     5761 aatccttccc gaacgctcca aatccataac tgagttttgt agtcattgaa atgctcatag
     5821 ggtatcgggt cgctgttacc aacgcccgcc ttctgttatt ggtatcgtca gaggtgtgcg
     5881 ccggaataaa tcccccggcg cagtagcgtt tagtcgcgtt ttaacggtgc aaccagcctg
     5941 gcagccagtc gccgtgggcg gcaatcaggt cgtcaacaag agcatatatt tcgtcaatgc
     6001 ccagcacggc ggcagtatgc gggtccatca tcgcagcgtg gtaaacacgg tcgcgatttt
     6061 ccgtaagaat agcttcggtc agcagcgtct gtacgttgat gttggtttgc atcagggcgg
     6121 ccagatgcga aggtagcgta ccgactttgg tcggctgaat gccattagca tcaaccagac
     6181 aggccacttc cacgcaacat ccttgtggca ggttatcaat caaaccatcg ttacggacgt
     6241 tgccgtaaat cacactcggc tcgcccgtcc agatagcgtt catgattgtg ctggcatatt
     6301 cccgtgacgg tttaatatca atccgggagg cgtttttata ctcctccagc tctttatgcc
     6361 agttcgccag ctgctcgacg cagcgtttcg ggtactcatc cagcggtact ttataacgct
     6421 caatcaaatc ctcacgacct ggcttaataa accacggtgt gtactcagca aaatgttctg
     6481 acgattccgt gacgaaatag cccagctttt tgaacatttc atagcgcaca atattctggc
     6541 agcgagtatt gccgtgaata ttcggcttcg gtgcctgccc tgcgtcataa gccgccagca
     6601 gttccgggta gagattcacg taactgccgt cggcggtttt gcgctccagc tccaggtaaa
     6661 acgccatatg gttgatacct gcgcaacggt aacgcagcgt agccgggtcg atattgaggt
     6721 cacgcgccag ctcttccgcc gttccctgca ccgaatggca cagcccgacc tgtttgatat
     6781 gcggatagcg ggcatacatc gcccaggtat tcatcgccat tgggttaaca tagttgagca
     6841 tggtggcatc ggggcagact tccgtcatgt cctcgcaaat ttgccacaga tgcggaatgg
     6901 tacgtagcgc gcgcataata ccgcccggcc ccaacgtatc ggcaatggtt tgttccagac
     6961 catgccgctt acagacctcg aaatcagtca ccgtgcaagg tgcataaccg ccaatctgaa
     7021 atgccaccac gacaaaatcg gcatcctgta aggcttcttt ctgttgggtg tggcaggtga
     7081 ttttgccgct ggcccctgct gaatccatca gcttacgcac cacaatatgc gactcttcca
     7141 ggcgggtggg gtcaatgtcc atcagggcaa tatgcgccgt tttcagcgcc tcgcgatgga
     7201 acacatcacc aagaatattt ttaacgaaaa tcgtcgaacc agcgccgata aatgtaattt
     7261 tgggtgcaga catcatgcag atctcctggc ttgcttgaat aacttcatca tggcaggcga
     7321 atctgcagaa aacctccggc ttccaggttg agcattccca taaactcaga tcctgcgtga
     7381 agcagcagta aatctgagtt tatgggaata atttacctga aaagcagagt tttcggcctc
     7441 atgatgctcg gacactcccc tcgcccctct gggaagaggg tgaaaaccgt gctcccactc
     7501 gcagtcatcc tccctcactc ctgccataat tctgatattc caggaaagag agccatccat
     7561 gaatacagat acgtttatgt gcagcagcga cgaaaaacag acccgcagcc cgctgtcgct
     7621 gtattcggaa taccagcgga tggagattga gtttcgcgca ccgcatatca tgcccaccag
     7681 ccactggcat ggtcaggtcg aagtgaatgt gcctttcgat ggcgatgtgg aatacctgat
     7741 caacaatgaa aaagtgaata tcaatcaggg acatatcacg ctgttctggg cctgtatacc
     7801 gcaccaacta acagataccg gaacctgtca gagcatggcg atttttaatc tgccgatgca
     7861 tctgtttctc tcctggccgc tggataaaga cctgattaac cacgtcactc acggcatggt
     7921 gatcaaatca cttgctacac agcaacttag cccgtttgaa gtgcgccgct ggcagcagga
     7981 attaaacagt ccgaatgagc aaattcgcca gctcgccatt gatgaaattg gcctgatgct
     8041 caagcgattt agcctctctg gctgggaacc gattctggtc aataaaacct cgcgcacaca
     8101 caaaaacagc gtctcgcgcc atgcgcaatt ttatgttagc cagatgctgg gctttattgc
     8161 cgaaaactat gaccaggcgc tgaccatcaa cgatgtggct gagcacgtca aacttaacgc
     8221 caactatgca atggggatat tccagcgggt catgcaattg acgatgaaac agtacattac
     8281 cgcgatgcgc atcaaccacg ttcgcgcgtt actgagcgat accgataaaa gtattctcga
     8341 tattgccctg acggcaggct ttcgttcaag tagtcgtttt tacagcacgt tcggcaaata
     8401 tgtcggcatg tcgccgcaac aataccgcaa acttagccaa cagcgccgcc agacgtttcc
     8461 cggctaaaga cgatatcagt atcagccaaa aaaatagttt agccgtcgat aataagcagt
     8521 gaaaataatt taaaataatc acataacata ttgtttataa aaacaaatat ttcaaaaatg
     8581 tttgtttttc acgcgcttta cagcccgaaa aggccggaag atacttgccc gcaacgaaga
     8641 ttccttcata accgggtaag caatgatgaa agtattaatt gttgaaagcg agtttctcca
     8701 tcaagacacc tgggtcggta acgctgttga gcgtctggca gatgctttaa gccagcaaaa
     8761 tgttaccgtg attaaatcca cctcctttga tgatggtttt gccattctct cttcaaacga
     8821 agccattgac tgcctgatgt tcagctatca aatggaacat ccggacgaac atcaaaacgt
     8881 cagacaattg atcggtaagc ttcatgagcg ccaacaaaac gtgccggtct tcctgttggg
     8941 cgatcgggaa aaagccctcg ccgcaatgga ccgggatctg ctggagcttg tcgatgaatt
     9001 cgcctggatt ctggaagata ccgccgactt tatcgccgga cgcgccgttg ccgcgatgac
     9061 ccgctaccgc cagcagctgt tgccgccact gttcagcgcg ctgatgaaat atagcgacat
     9121 ccatgaatat tcctgggcag cgccaggcca ccagggcggc gttggtttta ccaaaacacc
     9181 cgccggacgt ttctaccatg actactacgg tgaaaatctg ttccgcaccg acatgggcat
     9241 cgaacgaact tccctcggtt ctttgcttga ccatactggc gcatttggcg aaagcgaaaa
     9301 atatgccgca cgcgtatttg gtgccgatcg ctcctggtcg gtagtcgtcg gtacttccgg
     9361 ctctaaccgc accatcatgc aggcttgcat gaccgataac gatgtcgtgg tcgttgaccg
     9421 taactgccat aaatccatcg aacaaggttt gatgctgaca ggcgcgaaac cggtctacat
     9481 ggtgccaagc cgcaaccgct acggcattat cgggccaatc tatccgcagg aaatgcaacc
     9541 tgaaaccttg cagaagaaaa tcagtgaaag cccgctgact aaagacaaag ccgggcaaaa
     9601 accgtcttac tgcgtggtga ccaactgcac ctatgacggc gtgtgttata acgctaaaga
     9661 agcgcaggat ctgctggaaa aaacctccga tcgtctgcac tttgacgaag cctggtacgg
     9721 ctatgcacgt ttcaacccga tctatgccga tcactatgcc atgcgcggcg aacctggcga
     9781 tcacaacggt cctaccgttt tcgccaccca ctccacccac aaactgctga atgcgctttc
     9841 acaggcctct tatattcatg tacgtgaagg tcgtggggcg attaacttct cccgcttcaa
     9901 ccaggcctac atgatgcatg ccaccacctc cccgctgtat gccatctgcg catccaacga
     9961 cgtggcggtg tcgatgatgg acggcaacag cggcctgtca ctgacgcagg aagtgatcga
    10021 cgaagcggtt gatttccgtc aggcgatggc gcggctgtat aaagagttcg ccgctgacgg
    10081 tagctggttc ttcaaaccgt ggaacaaaga agtcgtcacc gacccacaaa ccggcaaaac
    10141 ctatgacttt gctgacgcac caaccaaact gctgaccacc gttcaggact gctgggtaat
    10201 gcatccgggc gaaagctggc acggcttcaa agatattccg gataactgga gtatgctcga
    10261 cccgattaaa gtcagcatcc ttgctccggg aatgggtgaa gatggtgaac tggaagaaac
    10321 cggtgttccg gcggcgctgg tcactgcctg gcttggtcgc cacggcattg tgcctacccg
    10381 caccactgac ttccaaatta tgttcctgtt ctctatgggc gtaacccgtg ggaaatgggg
    10441 gactctggtt aacacccttt gctccttcaa acgccactat gacgccaaca caccgctggc
    10501 gcaggtgatg ccggaacttg ttgaacaata tcctgacact tacgcgaaca tggggattca
    10561 cgatctgggt gacaccatgt ttgcctggct gaaagaaaac aaccctggcg cacggttgaa
    10621 cgaagcctat tccggcctgc cggtggcgga aatcaccccg cgtgaagcgt acaacgcgat
    10681 tgtcgacaac aatgtcgaac tggtatccat tgaaaatctg ccaggacgta tcgcggcaaa
    10741 ctcagttatc ccgtatccgc caggaatccc gatgctgctg tctggtgaaa acttcggcga
    10801 taaaaacagt ccgcaagtaa gttatttacg ctcgctgcaa tcctgggacc atcatttccc
    10861 tggatttgaa cacgaaactg aagggactga aattattgac ggtatttacc acgttatgtg
    10921 tgtgaaagcg taaccactat tccgctgaag gcgtaattgt ttaaataaca ttacgccgcc
    10981 tggccttagg ccttttgagt atggcaacgt tttcataaaa attgctgcaa acaaaaatgt
    11041 catacttttt gcgcggcccc accccgcgct t
//
LOCUS       DAZKIF010000136        11042 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZKIF010000136 DAZKIF010000000
VERSION     DAZKIF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.137"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            45..149
                     /gene="speFL"
                     /locus_tag="ACHOTW_003876"
     CDS             45..149
                     /gene="speFL"
                     /locus_tag="ACHOTW_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFP9845899.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            460..2658
                     /gene="speF"
                     /locus_tag="ACHOTW_003877"
     CDS             460..2658
                     /gene="speF"
                     /locus_tag="ACHOTW_003877"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP9845900.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2655..3974
                     /gene="potE"
                     /locus_tag="ACHOTW_003878"
     CDS             2655..3974
                     /gene="potE"
                     /locus_tag="ACHOTW_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP9845901.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4031..5671)
                     /gene="pgm"
                     /locus_tag="ACHOTW_003879"
     CDS             complement(4031..5671)
                     /gene="pgm"
                     /locus_tag="ACHOTW_003879"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP9845902.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5697..6242)
                     /gene="seqA"
                     /locus_tag="ACHOTW_003880"
     CDS             complement(5697..6242)
                     /gene="seqA"
                     /locus_tag="ACHOTW_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP9845903.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6427..7191
                     /gene="ybfF"
                     /locus_tag="ACHOTW_003881"
     CDS             6427..7191
                     /gene="ybfF"
                     /locus_tag="ACHOTW_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9845904.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7331..7624
                     /gene="ybfE"
                     /locus_tag="ACHOTW_003882"
     CDS             7331..7624
                     /gene="ybfE"
                     /locus_tag="ACHOTW_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP9845905.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7764..8294
                     /gene="fldA"
                     /locus_tag="ACHOTW_003883"
     CDS             7764..8294
                     /gene="fldA"
                     /locus_tag="ACHOTW_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP9845906.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8504..8590
                     /gene="uof"
                     /locus_tag="ACHOTW_003884"
     CDS             8504..8590
                     /gene="uof"
                     /locus_tag="ACHOTW_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP9845907.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8583..9029
                     /gene="fur"
                     /locus_tag="ACHOTW_003885"
     CDS             8583..9029
                     /gene="fur"
                     /locus_tag="ACHOTW_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP9845908.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9113..9439)
                     /gene="chiQ"
                     /locus_tag="ACHOTW_003886"
     CDS             complement(9113..9439)
                     /gene="chiQ"
                     /locus_tag="ACHOTW_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP9845909.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9489..10894)
                     /gene="chiP"
                     /locus_tag="ACHOTW_003887"
                     /pseudo
     CDS             complement(9489..10894)
                     /gene="chiP"
                     /locus_tag="ACHOTW_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2676 a         2746 c         2757 g         2863 t
ORIGIN      
        1 agaataactc taccttcaac tgacacagca agaggtaaag gtaaatggaa aataataacc
       61 gcttaatgcc tcatataagg cggacaaccc atatcatgaa gtttgcccat cgtaatagtt
      121 ttgactttca tttctttaat gcccgctagt cttctgacta aagggcaccc cagcgtacag
      181 gtctccctga ctttaagcat tacaggttaa tacctgtatc cctcggtgct catatactgc
      241 taaccctttt aaactctaca taattcgagt cgcagcatat gcaacttgaa gtatgacgag
      301 tatagccagt taccgggctg gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt
      361 atttatcagc ggtggagctt tgcttttttt ccggcgtgat cgatttctcc tttgagaaat
      421 tgaggacctg ctattacctg aaataaagag atgaacaaaa tgtcagaatt aaaaattgcc
      481 gttagtcgtt cttgcccgga ttgtttttcc actcatcgtg catgcgtgaa tatagacgaa
      541 agtaattata ttgacgttgc cgccattatt ttatcagtca gtgatgttga acgtggaaaa
      601 ctcgatgaaa tagacgctac tggctatgac attcctgttt ttattgcaac ggaaaatgaa
      661 gaacgtatac cagcagaata tctttcacgt atttctggtg tcttcgaaca tggagaagca
      721 cgcaaagaat tttatggtcg tcagttagaa accgctgcca gccattatga aactcaactg
      781 cgcccacctt tctttcgcgc actggtcgat tatgtcaatc agggcaacag cgcatttgac
      841 tgccccggtc atcagggcgg cgaatttttc cgtcgccatc cggcggggaa tcagtttgtg
      901 gaatactttg gtgaggcgct gttccgtgcc gacttgtgca acgccgacgt agcgatgggc
      961 gatctgctga ttcacgaagg cgcgccatgc attgcacagc aacatgcagc aaaagtgttt
     1021 aatgccgata aaacctactt cgttttaaat ggcacttcat cttctaacaa agtggtttta
     1081 aacgccctgc tgacaccggg tgatctggtg ctatttgacc gcaataacca taagtctaac
     1141 caccacgggg ccttgctaca ggctggtgca acaccggttt atctggaaac agcacgtaac
     1201 ccgtatggtt ttatcggtgg tattgatgca cactgcttcg aagaaagtta tctgcgtgaa
     1261 ctgattacag aagttgcacc gcaacgggca aaagaggcgc gtccgttccg cctcgctgtg
     1321 attcagctag gcacctacga cggtacgatt tataacgccc gccaggtggt ggataaaatt
     1381 ggtcatctgt gtgactacat cctgtttgat tccgcatggg tcggctatga acagtttatt
     1441 ccgatgatgg ctgactgttc gccgctgttg ctggatctta atgagaacga tccgggtatt
     1501 ctggttacgc aatccgtaca taaacagcag gctggttttt ctcaaacttc acaaatccat
     1561 aaaaaagaca gccacatcaa agggcaacag cgttatgtac cgcacaaacg catgaacaac
     1621 gcctttatga tgcatgcctc caccagcccg ttctatccgc tgtttgccgc actggatatc
     1681 aacgccaaaa tgcatgaagg tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat
     1741 ggcattaatg cccgcaaact gatcctcgat aactgtcagc atattcgtcc gttcatacct
     1801 gaactggtgg atggtaaacc ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg
     1861 cgcttcttcc agtttgtacc aggtgagcac tggcattctt ttgaaggcta tgcagagaat
     1921 caatactttg ttgatccgtg caaactgttg ctgacaaccc caggtattga tgcacgtaac
     1981 ggcgaatatg aagcgttcgg tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat
     2041 ggcgtagtgc cggaaaaatg cgatcttaac tccatcctct tcctgctgac tccggcagaa
     2101 gatatggcca aacttcagca acttgttgcc ctgctggtac gcttcgaaaa actgctggaa
     2161 tctgatgcgc cgctggcgga agtgctacct tccatctaca aacagcatga agagcgctac
     2221 gccggttata cccttcgtca gttgtgccag gaaatgcatg atttgtatgc ccgccacaac
     2281 gtgaaacaac tgcaaaaaga gatgttccgt aaggaacact tcccacgcgt cagcatgaat
     2341 ccgcaagaag ctaactacgc ctatttacgc ggtgaagtgg agctggttcg tctgccggat
     2401 gcagaaggcc gtatcgctgc cgaaggtgcg cttccttatc ctccgggtgt gctgtgtgtt
     2461 gttccgggtg aaatctgggg tggtgctgtt ttgcgttact tcagcgctct ggaagaaggg
     2521 atcaacctgc tgccaggttt tgcaccggag ctgcagggtg tctatatcga agaacatgat
     2581 ggtcgtaagc aagtttggtg ctatgtcatc aagcctcgtg atgcgcaaag caccctgctg
     2641 aaaggggaaa aattatgagt caggctaaat cgaacaaaat gggcgtcgtt cagttaacca
     2701 tactgacgat ggtcaacatg atgggctccg gtatcatcat gctgccgaca aagcttgccg
     2761 aagttgggac aatctcaatt atctcctggc tggtgacagc cgtaggctca atggcactgg
     2821 catgggcatt cgcaaaatgc ggtatgttca gccgtaaatc aggcggtatg ggcggttatg
     2881 ccgagtatgc tttcggtaaa tccggtaact ttatggcgaa ctatacctac ggcgtctcac
     2941 tgctgattgc taacgtcgcg attgctattt cggcggttgg ttacggcacc gaactgctcg
     3001 gcgcgagttt gtcgccagtg cagattggtc ttgcgaccat cggggtgctg tggatttgta
     3061 ccgtggctaa ctttggtggt gcgcgcatta ccgggcaaat cagtagcatt actgtgtggg
     3121 gagtcattat tccggtcgtc ggtctgtgca ttattggctg gttctggttt agcccgacgc
     3181 tgtacgttga ttcctggaat ccgcatcatg caccgttctt cagtgcggta ggttcttcca
     3241 tcgctatgac gctgtgggct tttcttggtc tggagtctgc gtgtgcaaat actgatgtag
     3301 tggaaaaccc ggaacgtaat gtgccaatcg cggtactcgg cggtacgtta ggtgcggcgg
     3361 tgatttatat cgtctccacc aacgtgattg ccgggattgt gccaaatatg gagctggcaa
     3421 attcaacggc accatttggt ctggccttcg cgcagatgtt cacgccggaa gtgggtaaag
     3481 tcattatggc gctgatggtg atgtcctgct gcggttcgct gcttggctgg cagttcacca
     3541 ttgcccaggt gtttaaatct tcatctgatg aaggctactt ccctaaaatt ttctcccatg
     3601 taaccaaagt ggatgcaccg gtgcagggaa tgttgaccat tgtgattatc cagagcgggt
     3661 tggcgctgat gacgattagc ccgtcgctaa acagtcagtt caacgtgctg gttaacctgg
     3721 ccgtggtgac caatatcatt ccgtatattc tgtcaatggc ggcattagtc attattcaga
     3781 aggtcgctaa tgtgcccccc tcaaaagcga aagttgcaaa ctttgttgct tttgttggcg
     3841 cgatgtacag cttttatgcg ctgtactcat ccggggaaga agccatgctg tacggttcca
     3901 tcgtgacctt ccttggttgg acactgtatg gtctggtatc accacgcttt gaactgaaaa
     3961 ataaacacgg ttaaaaaagc aaagtaaaaa agggcgatct tgcgaccgcc ctttttctta
     4021 tttaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct tctttctcaa
     4081 tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat gcgtcttccg
     4141 tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga ccgccaatag
     4201 aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacca gccagggtgc
     4261 tggcactcac catttccgga gacagcttag acagcgccgc tttttgtgcg gaagtcgcag
     4321 ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg ttgtagtgtt
     4381 cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac atgatgatgc
     4441 cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc cctgcgctct
     4501 cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat ttgaaaccta
     4561 ccgggacttc taccagttta cggcccaagt cgttgaccac acggtcgatc atcgcagatg
     4621 aaaccagcgt tttaccgacg gcaacagctt tgccccactg cggacgatgc tggaacaggt
     4681 agttgattgc caccgccagg tagtggttcg gattcatcaa acctgccgga gtgacgatac
     4741 cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca cgcagtgcca
     4801 gcaggcccgc catcgcacac tcggaggagc agtccatacg gatcgcgccg tctttatcaa
     4861 ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg ttgaggttgt
     4921 aatactcgcc aatacgcttc cagtattcga taccggaacc gcccagcgga tcaacgccaa
     4981 gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc ccttccacaa
     5041 acggctgcac cagatcctgc tctttcacat gaccggatgc catcgcttcg tcgagggaga
     5101 tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggctctgtct tccaccactt
     5161 tagtgacgtt ggtatcagcc gggccaccat ttggcggatt gtacttgata ccaccatctt
     5221 ccggcgggtt atgggacggt gtaataacga taccgtctgc cagcgggcca ccttttttat
     5281 tgtgaaccag gatggcattg gaaacggcag gcgtcggggt gaagccattg ttttcctgca
     5341 caataacatc tacgccgttc gctgccagca cttccagaac ggaaataaat gcaggttcgg
     5401 acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt ttcgcacgtt
     5461 cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg tggcgcgctg
     5521 cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc cctgcttctg
     5581 gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca ctctgttgtg
     5641 caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc aacgttttag
     5701 atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg catgatgtgt
     5761 tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata cggcgtgcct
     5821 ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc atctgccgca
     5881 aagtaaacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc ctgggcgtca
     5941 agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt ttgctctgca
     6001 tattcatccg aaagtagaag ttcacgcatt gcgcgaacct tgtctttaat cgttttgatc
     6061 ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac cggagcagca
     6121 ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc gctctcgccg
     6181 atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc aatcgttttc
     6241 atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caggagtata tgaataaatc
     6301 ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac cttatttgtt
     6361 gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga aaaaaagaac
     6421 tttgccatga aattgaatat ccgcgcgcaa actgcacaaa accagcacaa taattctccc
     6481 atcgttcttg tccatggtct gtttggcagc ctcgacaacc ttggcgtact ggctcgcgat
     6541 ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct ttcaccgaga
     6601 gatccggtaa tgaattaccc ggcgatggcg caggatcttg ttgatactct ggatgcacag
     6661 cagatcgaca aagcaacatt tatcgctcac tccatgggcg gtaaagcggt aatggcactt
     6721 actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc accggtcgac
     6781 tatcacgtac gccgtcatga tgagattttc tcggctatca acgcggtcag tgaatcggac
     6841 gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga agaaggggtg
     6901 attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt gccggtattg
     6961 tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga tcaccctgcc
     7021 ctgtttatcc ctggtggtaa ttctccgtat gttagcgagc agtaccgtga tgatttactg
     7081 gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg ggtccatgct
     7141 gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta acgcaactga
     7201 ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac tcatcgcgct
     7261 gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt cccccgaagt
     7321 caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt tcgcgcacga
     7381 gcgtcgaccg ggacgaccga aaacaaatcc gctttcgcgc gatgaacagc tgcgtattaa
     7441 taaacgcaac caactaaaac gcgacaaagt acgtggccta aagcgtgtcg aactgaagct
     7501 gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgcaata tgagccgtag
     7561 cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc agggcatcgt
     7621 ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg attccgcaag
     7681 actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc catttcaata
     7741 agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc agcgacaccg
     7801 gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac gttgccgatg
     7861 tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttatgacatt ctgctgctgg
     7921 gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc ttcccgactc
     7981 tcgaagagat tgatttcaat ggcaaactgg ttgcgctgtt tggttgtggt gaccaggaag
     8041 attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt gaaccgcgcg
     8101 gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca tcaaaaggtc
     8161 tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag ccggaactga
     8221 ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat ctcgacgaaa
     8281 ttctcaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta atagataatg
     8341 ccaatcaaaa taattgctac aaatttgtaa cttttgccgt tgtacctgta caatgtcccg
     8401 gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat taacaatatt
     8461 tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac gcattatctc
     8521 aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag gacagattcc
     8581 gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg cttcctcgtt
     8641 taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg gaagatttat
     8701 acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat cgcgtactga
     8761 accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt aaatccgtat
     8821 ttgaactgac acagcaacat caccacgatc acctgatctg cctcgactgc ggcaaggtta
     8881 tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca aaacatggca
     8941 ttcgcctgac taaccacagt ctctatcttt acggtcactg cgccgaaggc gattgccgcg
     9001 aagatgagca cgcgcacgaa ggcaaataag ccagtctgaa cgagaaaagc caacctgcgg
     9061 gttggctttt ttatgcaagg gaaataagaa aagcgagagt tacagctctc acttatttgt
     9121 tattactgcg aatttcctgc cagaccttat cacaatcggc tttcaccgcc tgatcatttc
     9181 cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcggacactt tcctggtcgg
     9241 caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg caggcttcaa
     9301 ttttttccgg cgaaccttgt gcggtgttga tacaggcgct gtatgcctct ttcagacggc
     9361 tgtcttcctg tggcgcggtt gattgcgcac aagctaccag ccccgatgcc atcatggcga
     9421 tgagaatcag ttttttcata gcaaattcct caaacgtgcg gcaggcacaa aacctgccgc
     9481 gtcgggcatc agaagatggt gaatggtgcg attaccataa attttacgtc acgctcatcc
     9541 tggaagatgt tgccgtaacc gccgccccag cttgggatgt cggagtggtt gtcgtattcg
     9601 gtgaagtgca gtttgaacat cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca
     9661 tccaggctgt atgcagactc ttcaatagtc cggtttttgt cgtagtacgc atccggattg
     9721 ctctgccagg tcgcaggttt agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag
     9781 cctggaagat tccagttttt caggtcatac atcgcaccga agaagaccgc tttttcgccg
     9841 ttggcgttga agtcagaacg gttatcccac cagatatcca ggcgaccgtt cgaggaagcg
     9901 taggttggag tcatacgttg caggaagtat ccctgctgac cgtcagcttt aacccaggtg
     9961 ccttcgaggc gcaaatccac tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc
    10021 caggcggtgc cgtcataaag atcgttgacg ctgcgatcgt caactttatc gcgcgtaccg
    10081 tagaactggt agctggtggt taacgggcta ccggcgatat caaatttgta gctggctttg
    10141 gcaaagtatt gatcgatata cccttccgcc tgaccaaatg ccgcttccag tacgaagtta
    10201 tttttgaagt cgtatttcgc cccaagggag tgcagataat caactttggt ggttttatcg
    10261 ttctgataaa actcatccat ttccagatgc cacggtgctt tgtattcgtt ggtccacatg
    10321 taggagaaac tcaacgcacc agcatcgccg taatcaaaat tcgccccggc ctccgcaccc
    10381 tgataagtac ccggcataaa gctccagtgc ggcgctaaca gcgtttgacc agttggctga
    10441 atataacctg ccctcgccac accggaccgt atttaaattt ggccgctgct ttatacaggc
    10501 ttataccgct tttatcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg
    10561 cgatttcgtt cgggtgagag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcgg
    10621 caatatcaag gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcgttcc
    10681 aggtggagtg agaaaggttg gttttgtatt tatcgccgtc ggtaacatct ttacggtcgc
    10741 gttcacgctg ccagtaatag ataccgccgg ttaaggttga atcgtcaatg aatccttcgg
    10801 cgcgagcctc cggcattgcc ataaagcccg acattgctgt aacaccggcg atagccagcg
    10861 ccagcgtact acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat
    10921 tgctgcacca caggtgaaca gcgaaaaaaa taaaaattaa aaaagtagcg ttgagaaaag
    10981 tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc tctttctgta
    11041 ac
//
LOCUS       DAZKIF010000137        10958 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZKIF010000137 DAZKIF010000000
VERSION     DAZKIF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.138"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            102..716
                     /gene="evgA"
                     /locus_tag="ACHOTW_003888"
     CDS             102..716
                     /gene="evgA"
                     /locus_tag="ACHOTW_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP9845910.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            721..4314
                     /gene="evgS"
                     /locus_tag="ACHOTW_003889"
     CDS             721..4314
                     /gene="evgS"
                     /locus_tag="ACHOTW_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP9845911.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEIIQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNVDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQNN"
     gene            complement(4370..5511)
                     /gene="yfdE"
                     /locus_tag="ACHOTW_003890"
                     /pseudo
     CDS             complement(4370..5511)
                     /gene="yfdE"
                     /locus_tag="ACHOTW_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(5585..6529)
                     /gene="yfdV"
                     /locus_tag="ACHOTW_003891"
     CDS             complement(5585..6529)
                     /gene="yfdV"
                     /locus_tag="ACHOTW_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP9845912.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6599..8293)
                     /gene="oxc"
                     /locus_tag="ACHOTW_003892"
     CDS             complement(6599..8293)
                     /gene="oxc"
                     /locus_tag="ACHOTW_003892"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP9845913.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8347..9597)
                     /gene="frc"
                     /locus_tag="ACHOTW_003893"
     CDS             complement(8347..9597)
                     /gene="frc"
                     /locus_tag="ACHOTW_003893"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP9845914.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10110..10745)
                     /locus_tag="ACHOTW_003894"
     CDS             complement(10110..10745)
                     /locus_tag="ACHOTW_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP9845915.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3203 a         2473 c         2258 g         3024 t
ORIGIN      
        1 aagctcaaat tttcttattt ttattgagaa aatgagatga tgccttatgt ctgtattact
       61 acagggagaa gggagatgct tcattgcaaa gggaataatc tatgaacgca ataattattg
      121 atgaccatcc tcttgctatc gcagcaattc gtaatttatt gatcaaaaac gatattgaaa
      181 tcttagcaga gttgactgaa ggcggaagtg ccgttcagcg ggtggaaaca cttaagcctg
      241 atatcgtcat cattgatgtc gatatccccg gagttaacgg tatccaggtg ttagaaacgc
      301 tgaggaagcg ccaatatagc ggaattatta ttatcgtctc cgctaaaaat gaccattttt
      361 acgggaaaca ttgtgctgat gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga
      421 acaatatcat tgcggctatt gaagctgcaa aaaatggcta ctgctatttc cccttctctc
      481 tcaaccggtt tgttggaagt ttaacgtccg accagcaaaa actcgactcc ttatcgaaac
      541 aagaaattag tgtcatgcgg tatattcttg atggcaagga taataatgac attgctgaaa
      601 aaatgttcat cagcaacaaa actgtcagca cttataaaag tcgcctgatg gaaaaattag
      661 aatgtaaatc actgatggat ctttacacat tcgcacaacg taacaaaatc ggctaaccac
      721 atgaagtttt taccctatat ttttcttctc tgttgtggtc tttggtcgac cataagtttc
      781 gcagacgaag attacatcga atatcgtggc atcagtagta acaaccgtgt cacacttgat
      841 ccactacgtc tgagcaacaa ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt
      901 gcagtacaca agtcccaaac ggctacgttg ttgcataccg attcgcagca acgggttcgt
      961 ggtattaatg ctgattattt aaatctttta aaaagagcgt taaatatcaa attaacactc
     1021 cgggaatacg cagatcatca aaaagcaatg gacgcgctgg aagaaggtga agtcgatata
     1081 gtgttatcac atttagttgc ttcgccgcct cttaatgatg acattgctgc aaccaaacca
     1141 ctgataatta cctttccggc gctggtcacc acccttcacg attcaatgcg accgcttacc
     1201 tcatcaaaac cagtaaatat tgctcgagta gcaaattatc cccctgacga ggtaattcat
     1261 caatcatttc caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc
     1321 gtctcagccg gacagaatga ttactttatt ggtagtaaca tcattaccag cagtatgatt
     1381 tcccgctatt tcactcactc cttaaatgta gtgaaatatt ataactcacc gcgtcaatat
     1441 aacttcttct taaccagaaa agaatctgtc attcttaatg aagtactcaa tcgatttgtt
     1501 gatgctttaa caaatgaagt tcgctatgaa gtatcacaaa attggcttga tacaggaaac
     1561 ctggcctttc tgaacaagcc attagaactc actgaacatg aaaaacagtg gattaagcag
     1621 catcccgatt taaaggtgct ggaaaatcct tactcgcccc cctattctat gacggatgaa
     1681 aatggctcgg ttcggggcgt tatgggggac attcttaata ttattacctt gcaaacaggt
     1741 ttaaattttt ctccgatcac cgtttcacac aatatccatg ctggaacaca gctgaatccc
     1801 ggcggatggg atatattacc cggtgctatt tatagtgaag atagagaaaa taatgtttta
     1861 tttgctgaag ccttcataac aacgccttac gtttttgtca tgcaaaaagc gcctgacagt
     1921 gaacaaacat taaaaaaagg aatgaaagtt gccattccat attattatga gcttcattcg
     1981 caattaaaag agatgtatcc ggaggtagag tggataaaag tcgataacgc cagcgctgca
     2041 tttcacaagg tcaaggaagg cgaacttgat gctctggtcg cgacacagtt aaattcacgt
     2101 tacatgatcg accattacta tcctaatgaa ctgtatcatt ttcttattcc cggcgttccg
     2161 aatgcatcgc tttcgttcgc ttttcctcgc ggagaaccgg aacttaagga tattattaat
     2221 aaagcactga atgcaatccc cccaagcgaa gttctgcgcc tgaccgaaaa atggattaaa
     2281 atgcccaatg tgaccattga cacatgggac ctctatagcg agcaatttta tattgttacg
     2341 acattatccg ttttattagt tggcagtagc cttttatggg gattctacct gttacgctca
     2401 gttcgtcgtc gtaaagtcat tcagggtgat ttagaaaacc aaatatcatt ccggaaagcg
     2461 ctctcggact ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa tgtcattagt
     2521 cacaatagtg catttgaaca ttatttcact gctgattact acaaaaatgc aatgttgcca
     2581 ttagaaaaca gtgaatcacc ctttaaagat gttttttcta atacgcatga agtcacagca
     2641 gagacgaaag aaaaccgaac aatatacaca caggtatttg aaattgataa tggcatcgag
     2701 aaaagatgca ttaatcactg gcatacatta tgtaatctgc cagccagcga acatgctgtt
     2761 tatatttgtg gttggcaaga tattaccgag acgcgtgatt taattcatgc actcgaagta
     2821 gagagaaata aagcgatcaa tgcaactgtc gcaaaaagtc agtttctggc aacaatgagt
     2881 cacgaaataa gaacaccaat aagctccatt atgggcttcc tggaacttct gtcgggttct
     2941 ggtcttagca aggagcaacg ggtggaggcg atttcactag cctacgccac cggacaatca
     3001 ctcctcggct taattggtga aatccttgat gtcgacaaaa ttgaatcggg taactatcaa
     3061 cttcaaccac aatgggtcga tatccctact ttagtccaga acacttgtca ctctttcggt
     3121 gcgattgctg caagcaaatc gatcgcatta agttgcagca gtacatttcc tgatcattac
     3181 ctggttaaga tcgaccctca ggcgtttaag caggtcttat caaatttact gagtaatgct
     3241 ctcaaattta ccaccgaggg ggcagtaaaa attacgacct ccctggttca cattgatgac
     3301 aaccacgctg taatcaaaat gacgattatg gattctggaa gtggattatc acaggaagaa
     3361 caacaacaac tgtttaaacg ttatagccaa acaagtgcag gtcgtcagca aacaggttct
     3421 ggtttaggct taatgatctg caaagaatta attaaaaaca tgcagggtga tttgtcatta
     3481 gaaagtcatc caggcatagg aacaacattt acgatcacaa tcccggtaga aattatccaa
     3541 caagtggcgg ctgtcgaggc aaaagcagaa caacccatca cactacctga aaagttgagc
     3601 atattaatcg cggatgatca tccgaccaac aggctattac tcaaacgcca gctaaatcta
     3661 ttaggatatg atgttgatga agccactgat ggtgtgcaag cgctacacaa agtcagtatg
     3721 caacattatg atctgcttat tactgacgtt aatatgccga atgtggatgg ttttgagttg
     3781 actcgcaaac tccgtgagca aaattcgtcc ttacccatct gggggcttac agccaacgca
     3841 caggctaacg aacgtgaaaa agggttaaat tgcggcatga acttatgttt gttcaaaccg
     3901 ttgaccctgg atgtactgaa aacacattta agtcagttac accaggttgc gcatattgta
     3961 cctcagtatc gccaccttga tatcgaggcc ctgaaaaata atacggcgaa cgatctacaa
     4021 ctgatgcagg agattctcat gactttccag catgaaacac ataaagattt acccgctgcg
     4081 tttcatgcac tagaagctgg cgataataga actttccatc agtgtattca tcgcatccac
     4141 ggtgcggcta acatcctgaa tttgcaaaag ttgattaata ttagccatca gttagaaata
     4201 acacctgttt cagatgacag taagcctgaa attcttcagt tgctaaactc tgtaaaagaa
     4261 cacattgcag agctagacca ggaaattgct gttttctgtc aacaaaataa ctaaatagtg
     4321 gctcccacaa tgttcaaatg tgggagctat ttaccagcac atcttacatt tatgatgaga
     4381 actcctggcg aatttgctcc ccatgctggt cgagcgttgc cgctcccggc ataacatgcg
     4441 ggtccgcgca gccactgatt tttatcggat tacccggcat cattattccc ccggcttcaa
     4501 tcaacatatt tctcgcctga gtttgtggca aattaatggc ctcagccaca cttaataacg
     4561 gcgcgacggg cacaccaact tcatgtattc tggctaacca aacttcagct gcctgtgttt
     4621 ttaacgtccg ctcaatatat tgtttaagaa tagtctggtt ttgtacgcgt aaaatattgc
     4681 tgctaaatct gggatcatta accagttccg taagctccag tgcctggcat aacgcagaga
     4741 aaagcttgtc attaccacaa caaatcgtaa tcggcttatc ctgagtatcg aaaacatcaa
     4801 aaggtgccat gtaggggtgg cgatttccca ggcgttgtgg tgacttccct gtcgcaatat
     4861 atgccatcag accatgctcc agaaaactca gcgtggcatc aaacatcgct atatcgacat
     4921 gcgcccctct ttggctcttt tcgcggccat aaagtgcact cactattccg ctgaataaat
     4981 aaacaccgcc gcatagatcc gcaagagagg taccaacacg cactggcgga gcatcagggt
     5041 accctgtttc catcattatc ccgctcattg cctgaatgat ggtatcgtag gcaggagcat
     5101 cttttagcgg accggtatgt ccgaaacctg acgatgaagc atatatgagg cgcgggttga
     5161 tttcttgtaa cctttcccat gaaagcccca gtttttccat tgtacctggg cgaaaattct
     5221 cagctaatac atcagcttgt ttaagcatat ttataaatat acttttatcg tgatcattct
     5281 ttaaatcaag aaccacactc tctttaccat gattaataaa actgtaatag agtgtccatc
     5341 cacataggga ccaaatgtgc gggtatcatc accatgaccc ggcggctcaa ctttaattac
     5401 ccttgcgccc atattacaaa gaagttgagt tccgaaaggt ccattaagga catgtgtcat
     5461 atcgataact aataagcctt caaacggccc tttgctttca ttatttgtca tttgcttatg
     5521 ccttgaaaaa agccgaaggt gaaaagacac ttcggcattt atgtaaaatc aataacatga
     5581 ttaattatga aaccagtcga ctgacataaa tccacaaggg agccgtgacg acaaaaccca
     5641 gtacgctcac agccaatgac gctgtaccag tgcgggtgta gacattaaac cggctggcaa
     5701 taatgattcc ggagaatgcc ggcggtaatg cgcctgccag taccatcatc tgcagatgtt
     5761 cgctgttcaa atgacatgcc ataccaacaa gaagcagcgc cagtggcatc agaatcagct
     5821 tcaggaaggt gttataagca atttcggcac tgaactcgaa tttatgtgca gccagagtca
     5881 accccgcagc gaataccgct acccctgagt tagccttcgc aatcagatta aaggttgggt
     5941 cccatgctgc cggaattttt accccaacca acaccaggat cgttgccaga acaggtgccc
     6001 atacaactgg ctcctttgcc gcagaaatta atgcgctcag attactattc ttcttaccat
     6061 ccgctcctga ggaaggattc agcaaataca gaccaatagg aatagtaatt gcgttaacaa
     6121 taatagaaat aattgctacc actaaacctg ttgatacgga atcaccataa ataggatcga
     6181 gaactgcaaa ccccaggaat ccaatggtag gtgaacctgc aattaatgca catacagctg
     6241 cttctgcatg ggtacgttta aaaaatttgt agcaaccaaa ccaggagaaa aagaaacatc
     6301 cgacaataac cacgagtgat acaagggtca gacgagtgtc cgcaaaaatc atttccctgt
     6361 ttgcccgggt aatagaaaca aatagggctg caggaagcgc gtagtttaat accagtttat
     6421 taaatgcccg ggcttgatct tctgaaaaag tttctcgtct gccgctaaaa taacccaaca
     6481 gcatgattac gataataggc aataaatcgc caataaaaaa tgttagcata atagtatttc
     6541 cgataagggt cccctcacca tcgcataatg agttggcaag aggagacgat gtcagtattt
     6601 aattaccagc gacttgtttt gggttaagtt tggtaatatg gccactttca gttcctgctg
     6661 cagggtcgat gaccacatta ataatggtcg gtttgcgcga ctgaataccg gtggttaaag
     6721 catgacgaag ttcatctgtc gtggtgacgt tatagccaac gccacgaaac gcatccatta
     6781 atttgtcata ccttgcatgg tgcaacagat ctgttggtga tggtgcacca gcgccactga
     6841 gatcaacacc gtctcctctg tagatgccgc cattattaaa aataacgatc gttaccggca
     6901 ggttatatcg acaaatcgtt tcaatttcca tcccactgaa accaaaagca ctatcacctt
     6961 caatggcgac aaccggagaa ccagaggtca cgctagcacc gatggcatag cccataccga
     7021 tgcccatgac accccaggta ccacaatcca gacgacgacg tggtttatac atatcaataa
     7081 tatttcgtgc attatccagg gtatttgcac cttcattaac taaataaata tcctggttct
     7141 cacgcaatac atcgcgcaca gcacttaatg cattaaagta atttaatggt tgggtatctg
     7201 tacttaattt ttcatgcatt ttttgtgcat tttgctgctt gtggatattt aaaatatcgc
     7261 gccataccag tggagtcgta aatgtgtttt gtttcagctc tgccagcata ccttgcatac
     7321 tggatgcaat atcgccaacg actggcacag caatggggcg gttgctgtca atttcctgcg
     7381 gttcaatatc cagttgaata aactgtgtat ctgccgccca tcctttttta ccgtgtgcca
     7441 gtaaccaatt cagtcgtgca ccaacaagca tgacaacgtc agcatttgcc agggcaaacg
     7501 aacgcgcagc tgccgcagaa agtggatgcg tatcttcgag gatccctttc gccatagaca
     7561 ttggcaggaa tggaatctgg gcactttcaa taaattcacg aagctgttcg tcagcttgtg
     7621 aatacgccgc gcctttgcca aggataatta atggccgttc agcttttgct aaaagcgaaa
     7681 ttgcgctagt gactgacttc gggcatggca ataatgctgg cgacggattt tcaactttta
     7741 caatcgtggt taacgcttcg tctttttcca tcgtcgcggc caggacattt gctggcaaat
     7801 caagataaac tccgccaggg cgacccgata ctgagacccg gatagcgcgt gccaatgcaa
     7861 tgccaagatc ctgcggctga ttaacgcgaa atgctgcttt ggcatacggt tttgccgcat
     7921 tcatttggtc cagctcttca taatcacctt gctgtaggtc gacgatcgcg cggtcgctgg
     7981 agccgctaat catgatcatc ggaaaaccat ttaccgttgc gttggccaat gcggtcaaac
     8041 cattgaggaa tcctggcgca gacactgtca ggcagatccc tggtttttgg gtaagaaaac
     8101 cgcttgccgc agcggcatag cctgccgact gctcatgacg aaaaccaata taacgaatgc
     8161 cttccgcctg ggcatggcgc gccatatccg tcacaggaat acctacaaca ccataaatag
     8221 tgtcaatatt attctgtttt aatgcttcaa cgatgatatg cataccatct gtcatttgaa
     8281 gttgatctga catgattaat aacctcgaaa tttttataaa ttgggagcca gaagcccccg
     8341 ttaatatcag atggcgtggt tttgcttcat tgcagcaatt tcatcgtcgc tataacccag
     8401 ctcctgcaat accgcagcgg tatgttcacc taatagaggc gcagctttaa tatccggcgt
     8461 aaaggcagag aatttcattg gacaaccaac tgtcagatat tttccacgca atggttgttc
     8521 cacttcgaca acactgccgc tttggcgtaa agagggatca agtgaaattt ctttcatact
     8581 taaaaccggt gcacaaggaa tatcaaactg agtcaaataa gccactgctt catgtttatc
     8641 aatagtgaca gtgtattttt cgatttcagc aaaaatatcg aaaatatgtg gctgtcgggc
     8701 atgggctgta ctgtatgccg gatcggtaat ccaatctggt ttgccgatgg ctttacaggt
     8761 gttttcccag ttttgctcct gaatagtgaa ataaatatag gcgttaggat cagtttccca
     8821 gcctttacat ttcaggatcc agccaggctg accgccacca cccgcattac ctccgcgggg
     8881 aactgcatca ccaaatgtgc cattcggata ctgcgggtat tcttccagat aacccaattt
     8941 atcgagacgc tgctggtcgc gtaatttcac gcggcaaagg ttcaatacgg catcctgcat
     9001 tgacatggtg actcgttgcc cacgccccgt tttttcgcga tgcagcaaag cagcaagtaa
     9061 accgatcagt aaatgcattc cggtgttgct gtcacccaac gctgcagcgc ttaccagcgg
     9121 cggaccgtcc caaaaacccg tagtggatgc cgcgccaccc gctgcctgag caacgttttc
     9181 ataggctttt acattcacat aaggcgaaca ctcatcaaac cctttgatcg aaccaaaaat
     9241 cagacgtgga ttgatttctt gaatatgctc ccaggtgaag cccatgtgat caatggcccc
     9301 tggatgaaag ttctcgacta agatatcagc ttcgcggatc agcttttcca ttacctcttt
     9361 gccttccgct gttttggtat ttaactcaat agaacgtttg ttactgttaa gcatggtgaa
     9421 gtaaagcgca tcgatatcag gaatatctcg cagctggtga cgcgttacgt caccaacgcc
     9481 ggggcgttca attttaatga cgtcagcgcc aaaccaggcc agcatttgag tacaagatgg
     9541 gccagattgc acaccggtga aatcgagaac tttaattcct tgaagtggag ttgacatagc
     9601 aatacctatt agttaattaa atatgtgtta atttagcgtt tatttaaggc ataacgccct
     9661 ctataaataa tccgcaaaat atcgtcatag cggcactcga tatttttaaa ttcctgtatc
     9721 aagtgctgaa aatcatttca tcgctacttc caattccttt ctgcatggta agtagtgagg
     9781 acgaaaagaa gaatatgata aatattggtt cgtggtaatc agagagagtc gcgacatttg
     9841 tgatgttatt cttacaactg taagaagcgc cggtgattta caagtaaggg tatgattacg
     9901 ctttttagag cgtaccataa aatataacat attaaatgat ttgtgatgac acgggattat
     9961 tgcggtttac tttgaggaat ataaaatgcg agtttataga acagcgagcg ccaaataatt
    10021 ttatgaccac aacctgtcgt attgtggtca taaaaaattg tcggctggag ggagttaaat
    10081 cctttacctc aatatactgc ctgtcagcat tagttcacaa acagtgcttc gctatcgacg
    10141 atgattccat cttccgcacc tttaagcgcc aggtttgcct cgtagtattg ctttttatca
    10201 agcagtttct gcgcatcagc aagtgcatta cgagtctgtt taagcggcat cagatattgg
    10261 ttttccatta caccaacacc ggccaggcga agctcttcca ttgcgccctt tttatcccct
    10321 ttcgccattt tttcattggc aatttttatc gcggcttctt tttcgggtgt cgctacataa
    10381 ctctcagaaa ttccgacaga tgcattaatg acaatatatt ggtcatcatt gacattggtc
    10441 tttttacctg gcttagcgaa ctttgcccat tcggtactgt catctgacaa caaagctgaa
    10501 gcttcattgg tcagttcttt tgccttttcc ggatcgccat gaaacagagc caggcgcgct
    10561 acctgcacgt cacgcatcgc atataaacct tgttcagaga tacgctctgc tgcagctgcc
    10621 gttttctggg tttgctgaac ttgctgagtt tgctgttgtg ccgctaccgg agcattatca
    10681 gcagcccata cagttgaaga tgccagaata gctgtgacca tcgtagccat aattaaacgt
    10741 ttcatactta cctcattctt taacagtttt agaaaacgcc gccatcatat tgggtgagca
    10801 tgaaatgaga aatcagagag caacggtcat tttgaaaggg ttatcttaca gttgtagtta
    10861 ttatcgcaag aagtatttta gtcttacaat tgtaggaata tgcttcctgt cacagagaac
    10921 tattcttatg taccacaaag agatgtcatt tattatat
//
LOCUS       DAZKIF010000138        10932 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZKIF010000138 DAZKIF010000000
VERSION     DAZKIF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.139"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..66
                     /locus_tag="ACHOTW_003895"
     CDS             <1..66
                     /locus_tag="ACHOTW_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9845916.1"
                     /translation="RLITMQKQLEGTQKYINEQCR"
     gene            complement(98..391)
                     /gene="bor"
                     /locus_tag="ACHOTW_003896"
     CDS             complement(98..391)
                     /gene="bor"
                     /locus_tag="ACHOTW_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFP9845917.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGTENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            541..744
                     /locus_tag="ACHOTW_003897"
     CDS             541..744
                     /locus_tag="ACHOTW_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845918.1"
                     /translation="MSTALTGNFSAGVCGDNQKRCTPGFLIFHEMGAISREAACPVRW
                     WKKPDKTTALCKYRSNMVLLCEI"
     gene            800..1606
                     /locus_tag="ACHOTW_003898"
     CDS             800..1606
                     /locus_tag="ACHOTW_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9845919.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTNQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            1587..3293
                     /locus_tag="ACHOTW_003899"
     CDS             1587..3293
                     /locus_tag="ACHOTW_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9845920.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            3293..5437
                     /locus_tag="ACHOTW_003900"
     CDS             3293..5437
                     /locus_tag="ACHOTW_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9845921.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPPEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLVVMSDEPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQI
                     ERPDGIIKLNPVRKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            5595..6602
                     /locus_tag="ACHOTW_003901"
     CDS             5595..6602
                     /locus_tag="ACHOTW_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845922.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSAQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            6626..7840
                     /locus_tag="ACHOTW_003902"
     CDS             6626..7840
                     /locus_tag="ACHOTW_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFP9845923.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            7896..8285
                     /locus_tag="ACHOTW_003903"
     CDS             7896..8285
                     /locus_tag="ACHOTW_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845924.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            8335..8796
                     /locus_tag="ACHOTW_003904"
     CDS             8335..8796
                     /locus_tag="ACHOTW_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845925.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAALAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            8780..9343
                     /locus_tag="ACHOTW_003905"
     CDS             8780..9343
                     /locus_tag="ACHOTW_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845926.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFF"
     gene            9343..9993
                     /locus_tag="ACHOTW_003906"
     CDS             9343..9993
                     /locus_tag="ACHOTW_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFP9845927.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVEWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALYARKKVFNGGGV"
     gene            9990..>10932
                     /locus_tag="ACHOTW_003907"
     CDS             9990..>10932
                     /locus_tag="ACHOTW_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFP9845928.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAVTAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGD"
BASE COUNT         2832 a         2514 c         3111 g         2475 t
ORIGIN      
        1 aggctgatca ctatgcaaaa acaactggaa ggaacccaga agtatattaa tgagcagtgc
       61 agatagagtt gcccatatcg atgggcaact catgcaatta ttgtgagcaa tacacccgcg
      121 cttccagcgg agtataaatg cctaaagtaa taaaaccgag caatccattt acgaatgttt
      181 gctgggtttc tgttttaaca acattttctg tgccgccaca aattttggct gcatcgacag
      241 ttttcttctg cccaattcca gaaacgaaga aatgatgggt gatggtttcc tttggtgcta
      301 ctgctgtctg tttgttttga acagtaaacg tctgttgagc acatcctgta ataagcaggg
      361 ccagcgcagt agcgagtagc atttttttca tggtgttatt cccgatgctt tttgaagttc
      421 gcagaatcgt atgtgtagaa aattaaacaa accctaaaca atgagttgaa atttcatatt
      481 gttaatattt attaatgtat gtcaggtgcg atgaatcgtc attgtattcc cggattaact
      541 atgtccacag ccctgacggg gaacttctct gcgggagtgt gcggggataa tcaaaaacga
      601 tgcacaccgg gttttctcat ttttcacgag atgggagcga tttcccgcga agccgcctgt
      661 ccggtgcggt ggtggaagaa accggataaa acaaccgcat tgtgcaaata tcgatcaaat
      721 atggtgctgc tgtgtgaaat ctgaaaaatc acagcggtca ttatgcatca gtttttaaca
      781 caggacgtca gaacgtgaca tggcaaagct ggactggaaa aagctggagc aggcattccg
      841 acgcgaacat gccgaaacgg gaataacatt actggactgg tgccggaaga aaaagattaa
      901 ttacaacacc gccagaaccc gtataaaaat gggcaaaatc gatcatgaaa ttgatcataa
      961 aaccgatcat gaaatcgatc atgacatctc agatgaagaa ccctgcaatg acgcgggttc
     1021 cggcgatgaa aaatgtgcaa aaaactctga aaaaaactgc gcaaattcgg cagaaacgaa
     1081 acggattcgt ggttcccgac ttttaccacc ttcaaacgct ttttctcagc gaaacaccca
     1141 cgccgtaaga caccgtggat atgcgaagta tcttgaggca gataacctca tggatgatgc
     1201 gtccgacatg gtgctgttcg atgaactggt gttcacccgg gcccgcgcac tttcagtaac
     1261 taaggcactt aaagggatgt tcgccgacct ggaagaggca actgacgtgg aaacccgtgt
     1321 tgctctttac gacaaaatac tcaaagctga acaggccctt gaccggaata ttgcccgtat
     1381 cgagtcaatt gaacgctcat tgctgacgct ggacgtcctg gctgagacag caccaaaact
     1441 tcgtgctgac cgggaaagaa tcaacgccgc cagagataaa ctcagagctg aaaccgatat
     1501 tctgaccaac cagcgtcggg gcgttgttac gcctgtcagt gacatcgtgt catcgctgca
     1561 tgaaatgagt aattcgggga gactggatga cattccggaa gaatgaaccg cgatgtgatg
     1621 agccgtcaga aatgaccgag gctgaacaac gtctgttcat catgactaaa ctgagcaatc
     1681 cctggtggcg gctcaatcat ctctacaaaa tacagaacga aaaaggtgaa ctggtcacct
     1741 tcagaatgcg accggcgcag cgccagttgt tccggagcat gcacaataaa aatattatcc
     1801 tgaaagcgcg ccagctggga ttttccacag ccattgatat ttatcttctc gaccaggcat
     1861 tattcattcc gcatctcaaa tgcgggatcg tcgctcagga taaacaggct gccagtgaaa
     1921 ttttccgcac aaaaattgct gtaccgtttg atcatctccc tgactggctg agagcctcat
     1981 tcaccatcgt tgaacgtcgt agcggtgcca gcggtggcta tatcctgttt ggtcacggct
     2041 cgagtatcca ggtggcaacc tcattccgtt caggtacggt gcagcgcctg catatctcag
     2101 agcacggcaa aatttgcgcg aaatatccgg ctaaggcgaa agaactgcga accggtacgc
     2161 ttaatgccgt ctctgatgaa tgcattattt ttgatgagtc cactgctgaa ggcgtgggtg
     2221 gtgattttta cgagatgagt aaccgagcac aggagatcac tgcatcaggc ttattgctga
     2281 cggcacagga ttataaattc catttttacg cctggtggca ggatcctaaa tacagcgcca
     2341 gagtgccgga aagcgggctg aagctgtcac gggaaaaaat gacgtatttt tctgcggttg
     2401 agaaggcaat gaacatcacg cttactgatg aacagaagca gtggtacatc aataaggaaa
     2461 ctgaacagcg tgaggaaatg aagcaggagt ttccctcaac gccacaggag gcgtttctga
     2521 cgtccggacg acgtgtgttc agtgccgaaa gtacgttgca ggcagaatca ttctgttcgc
     2581 caccgatgat tgtttatgac attgaacctg ttacaggagc gaagactaaa gctcagtctc
     2641 tgcgtgaagg aaataaaaac gagttgcagc ggacgctgat gaattatctg ctggtatggg
     2701 aactgccgga tccggatgaa gagtatgttt gtggggcaga tactgccgaa gggctggagc
     2761 acggagaccg ctcatcgctg gatgttgtca aacgcagtaa tggcgagcag gtggctcact
     2821 ggttcgggca tctcgatgct gaactttttg ctcatctcat ttcgcaggtc tgtcgtatgt
     2881 ataacaacgc gtttgtgggg ccggagcgta ataatcacgg acatgcagtt atcctgaaac
     2941 tccgggaact ctatccgaca cgttatatct acaacgaaca gcatcttgac caggcatatg
     3001 acgacgatac gccccgcctt ggctggctga caacccgtca gagcaaacct gttctgaccg
     3061 aaggaatgaa aacgcttctg aataatggaa tatcagggat ccgctggtca ggcacattat
     3121 cggaaatgaa cacctacgtt tatgacgcga aaggctccat gaatgcacag gaaggctgct
     3181 ttgatgatca gctcatgagc tacatgattg cccaggagat gcgcgccaga atgccggtga
     3241 gggtaaaaca gaaaacggat aaacgcagaa ccacacactg gatggctcac tgatgaaaaa
     3301 tgaaactaac accatggcga cgaaaaacga caatggagcc acgccgcgtt tttctcagcg
     3361 ccagttacag gcgctttgtt ctgatattga cagccagcct aaatggcgtg atgccgcaaa
     3421 caaggcctgt gcgtattacg atggcgatca gttgccaccg gaagttcttc aggtactgaa
     3481 agatcgcggt cagccgatga ctatccataa cctcatcgcg cctaccgtcg atggcgttct
     3541 gggaatggag gccaaaacac ggactgatct ggtggtgatg tcagacgagc cagatgatga
     3601 aactgaaaaa ctggctgaag ctattaatgc tgaatttgcc gatgcatgcc gccttggcaa
     3661 tatgaataaa gcccgctctg atgcctatgc ggaacaaatc aaggcgggcc tcagttgggt
     3721 ggaggtcaga cgaaacagcg atccgttcgg gcctgaattt aaggtgtcta ctgtcagccg
     3781 gaatgaggtt ttctgggact ggctgagccg ggaggctgat ttaagtgact gccgatggct
     3841 gatgcgtcgc cgctggatgg ataccgatga ggcaaaagct acattcccgg gaatggctca
     3901 ggttatcgat tatgccattg atgactggcg tggttttgtc gataccacgg ttactgaagg
     3961 ccagcccagt ccgttgatga gtgcatggga agagtatcag tcatgggatc gacagcagaa
     4021 cgaatggctt cagcgtgaac gccgtcgtgt gctgcttcag gtggtttatt accgtacatt
     4081 cgagcgtctt ccggtgattg aactcagtaa tggacgggtg gtggcctttg ataaaaataa
     4141 tctgatgcag gcggtagctg tggcatccgg gcgggttcag gtgaaagtcg ggcgggtaag
     4201 ccgtattcgt gaagcctggt ttgtcgggcc acactttatt gtggatcgcc cctgtagtgc
     4261 tccgcagggg atgtttccgc tggttccttt ctggggatac cgaaaggata aaaccgggga
     4321 gccatacggg ctaatttccc gcgccattcc ggcacaggat gaggtgaatt ttcgtcgtat
     4381 caagctgacc tggttgcttc aggccaaacg cgtgattatg gacgaggatg ccacccagtt
     4441 gtcagacaac gacctgatgg agcagatcga acgtccggat ggcattatta aactgaatcc
     4501 ggtccgaaaa aatcagaaaa gtgtcgcaga tgtttttcgg gttgagcagg attttcaggt
     4561 tgccagccag cagtttcagg tcatgcagga atcggaaaaa cttatccagg ataccatggg
     4621 agtgtattcc gcatttctcg ggcaggattc aggtgcgacg tcaggcgtgg ctatcagtaa
     4681 cctggtggag cagggggcca caacccttgc ggaaatcaac gataactacc agtttgcctg
     4741 ccagcaggtg ggaagactgt tgctggctta tcttctcgat gacctgaaaa aacgccgtaa
     4801 tcatgcagtg gtgattaatc gcgatgatcg ccagcgtcgc cagaccattg tcctcaatgc
     4861 tgaaggtgat aatggtgaac tgaccaatga tatttcaagg ttaaatacac atattgcgct
     4921 ggcgcctgtt cagcagacac cggcgtttaa ggcacagctt gcacagagaa tgtcagaggt
     4981 tattcagggg ctgccgcctc aggtgcaggc tgttgtgctc gacctgtggg ttaatcttct
     5041 ggatgtgccg cagaaacagg agtttgttga gcgtattcgt gctgcgctgg ggacgccaaa
     5101 atcaccggat gaaatgacgc cggaagaaca ggaagtagcg gcacaacaac aggcacttca
     5161 gcaacaacag gcagaactcc agatgcgcga gatggctggc agagtggcaa aactggaagc
     5221 tgacgccgcc agggcacatg cagctgcaca acgggataat gccagtgcac agcgggaagt
     5281 cgccctgaca caggggcagc gttatgtgga tgcgcttaac caggcacata cggcagaaat
     5341 cattaccggc gtacagaata tggaacagga gcaggacgtt cttcagcaac agatgctgta
     5401 tacgttacaa cagcggatga atgaaatgtc gctctgaaaa ctctggcttc aactgaaccc
     5461 cgtcatcgta cggggttttt tgtttccgga ggtaagcgtt ccgggagcgg tgcgcttatt
     5521 cgcgggggca gcgataagcc ttatttactc aaccattcgg atctgtccga taaacagacc
     5581 atgcggagtt atttatggat tttgaattta cgggtgaaga aaccccggaa caactggaaa
     5641 aaatgctgga aggacttggg gatgtggata ttgacagtca cgcacaggac gtcgtgacgg
     5701 aagataccac ggaaaaacat gcggatgagg aagcacagac tcagacgggc gataacaatg
     5761 tggcaccgac gccggatgcc agtgtggagc agacgcagga cgtgaaggag ccggaagcga
     5821 agggggtgct cacccgcgac ggtaaacacg tcattcccta tgaagtcctt gaggctgaac
     5881 gttccggtaa gcaacgggcc gaacaggaag ccgcacttct tcgtgggcag atagctgaag
     5941 aaaaacgcag ggtggaactg ctgacgtctc agatccacca ggccggtatg aagcccacac
     6001 cgttaccgga aaacgaaaaa atttctgatg agcagattgc ccgtatcagg gagatgtatc
     6061 cggaaattgg tgacgcggtg gcttcgctca tccgtaaaaa taactatctc cagtcccgtg
     6121 ttcagcaatc agcacagcag gcagaaggta atggtggtga ggatttatca ccggttcttg
     6181 atgcgatgaa tgccgtgccg gtgctgaaaa cgtggcagga gtccgatcca gatcgcttct
     6241 cggttgctgt atccatcgac gggaagctcc agaatgaccc cgcatggaaa gacaaaacgc
     6301 tcactgaacg tttcgctgaa gtggcccgtc gtacgcaggt tgctttcggt gaagtcagtg
     6361 agtcgtctgc tgacaacaag gcagacaaaa cggatatccg gaaaacggcg gaagagaaag
     6421 tgaagaccgc tgaacaggag caggcagtac ctgcttcccc gtcagattta ggcaccacgg
     6481 cttccgtcgg aaccggtgat aattttgaac ggttacttgg cgcttctcat tcagaggcag
     6541 aggcgattat gcgcggtatg acgaatgctg aaatagacgc gcttctggag aagctcgggt
     6601 aacttactga aggagaactg aagtaatgac gactgtaaca tcagcccagg cgaataagct
     6661 gtatcaggtg gcgcttttta ccgctgccaa ccgcaaccgc tcgatggtaa atatcctcac
     6721 tgaacagcag gaagcgccaa aagcggtttc gccggacaag aaaagcacga agcagaccag
     6781 cgcgggtgcg ccggttgtcc gtatcacaga ccttaacaaa caggccggtg atgaagtgac
     6841 cttcagcatc atgcacaaac tctcaaaacg tccgacgatg ggagatgagc gtgttgaagg
     6901 tcgtggtgag gatctcagcc atgctgactt ctccctgaaa atcaatcagg gacgtcacct
     6961 ggtggatgca ggcggacgta tgagtcagca gcgcacgaag tttaacctgg catcctcagc
     7021 cagaacgctt ctggggacgt actttaatga cctgcaggac cagtgtgcga tagtgcatct
     7081 tgctggagct cgtggtgatt ttgttgctga cgacactatt ctgccgacag cggagcaccc
     7141 tgaattcaaa aaaatcatga tcaacgatgt actgcctccg acacatgacc gtcacttttt
     7201 tggcggtgat gcgacaagct ttgagcagat tgaagcggca gatatttttt ctattggcct
     7261 ggtggacaat ctctccctgt tcattgacga aatggcgcat ccgttacagc cggttcgtct
     7321 gtccggtgat gaacttcacg gagaagatcc atattacgtc ctgtacgtca cgccgcgtca
     7381 gtggaatgac tggtacacct cgacgtccgg taaggactgg aaccagatga tggttcgtgc
     7441 cgtgaaccgt gcaaaaggtt ttaatcatcc gctgttcaaa ggtgaatgtg cgatgtggcg
     7501 caatatcctg gttcgtaagt atgcgggtat gccgatccgt ttctatcagg ggtcaaaggt
     7561 tctggtatca gagaataacc tgacggcaac cacgaaagag gtcgctgctg caaccaatat
     7621 tgaccgcgcc atgttactgg gggctcaggc gctggcaaat gcttacggtc agaaggcggg
     7681 cggtcacttc aacatggttg agaagaaaac ggatatggat aaccgtactg agatagcaat
     7741 cagctggatc aacggtctga aaaaaatccg tttccccgag aagagcggca agatgcagga
     7801 tcacggcgtg attgccgttg atacagcagt gaagctctga ttttttcctt tccctatgcc
     7861 gggttttcgc ccggcttttt caggagtcat taattatggc aaagactatc cttgccccgt
     7921 cactgagtga acgggtctat acgggtacgc acggtaatga gtcggtggca gaaggcgtat
     7981 ttacggtgaa tgctgcggaa gcggacagtg ttattcatct tctctcactg ccagtgggca
     8041 tccgtatcaa ctcactccaa ctggtttcaa cgggtggtct gggtactgca accgtcagca
     8101 ttaagtccgg tgagcatgct ctcatcgata acagcgaagc tgtttctgca aaatttgcca
     8161 gatatgtgcc agtggagccg tacaccacac agcgtgacgg ggagctggtt actgtcacca
     8221 ttaagactgc cgctgcaacc ggcaccctga atgttctgct gcgttatacc gtggtgggat
     8281 actgattaaa accttccggc ccgcgtcatg cgggcttttt atccggggaa ttatatgagt
     8341 gagaaaattg ccgttgtcta tatcggccca aaacccgtga aaaaggacac cattaccgga
     8401 agtcgcacgc tgttcccacg tcttgagccg gtgcatgttg acagcgcgat ggcctggcaa
     8461 ctgctggggt ttccggatgt ctgggttcgt catgaagagc ttgatgatgt tctgaaaaag
     8521 caacaacaga atgagcagtt gcggcaggca cagcaggcgc aggaaagagt gcttgctgcg
     8581 ctggcagaag cggagaacag ttttgttgtt tctgttaacg ggcaggaggt ggatttaagt
     8641 aagctcacct cagcacggct ggcgacgctg tgtgaggcag aagagctgga tattcacaaa
     8701 gacccgaaag aaacggctga ggcattccgt atccgggtgc gtgaggcatt tcgccgtcgt
     8761 gttgcggaga ctgaacagca tggcggaact gagtgatttt ttaccgtatg tccgtcgtca
     8821 tatcagcggt ccactgaaca ttatgatgac ggatgctctg tcaatggctg ccgtggcatt
     8881 cagccgccag tcgttggtgt gccgtcggga ggttactgtt gtaccagtag caggaaaaga
     8941 aatcgtgctt ccgtatgaca aagatgatga ggagtgcgtt catatcatcc gtatctctga
     9001 cgataatcat gagctttttg tcggtcggga tgtggatatc agctccggac gctccctgcg
     9061 atttgcctgt tctcccggtg aggtgagcgt gctttatgcc gtcgctccga aagccggacg
     9121 cagccagata ccggatgaac tcctcacatg gcctgaagaa gtggctgcgg gggcacttga
     9181 gcggttgttc atgcagactg gtgtttcatg gtcagatccg ttacgcgcac agtatttttc
     9241 tgtgcagttt tctgagggga tccgtcgggc atatcgtcat acactggcga caagcccgta
     9301 ctcttcatac cgcaaccctg tacgcaggca gaggtttttc tgatgacgac gattacagaa
     9361 atcatcggac gtgtgaatac acaactggtt gacccgatga tggttcgctg gcccctgcag
     9421 gaattgtgcg attattacaa tgatgctgtg agggcagtga ttctggcgag accggatgct
     9481 ggcgcaagcc tggaaacaat aagttgtgtt cctggcgccc gtcaggtttt gcccgatggt
     9541 gtaatacaac ttcttgacgt gatatgcctc agtgacggta gtgcagtcag accattatcc
     9601 cgggaggtgc tggatgcgca gtatcctgag tggcccacaa tgaagggtat tcctgaatgt
     9661 tttatcagca acgacctgtc cccgcgcgta ttctggctgt ttcctgctcc tgacaaagag
     9721 ataagtattg atgcagtggt aagccggata ccggaggcag tgtatgttct gacgcaggac
     9781 gatgatacgc cagttccact ggaagaggct tatgttaacc cactggtgga gtggatgttg
     9841 tttcgcgctt tcagtaagga tgctgccggt ggcgcagaat cggggctggc tgcgcagcat
     9901 tatcagagtt ttgttgagca acttgggatc aaacaggggg cagacagtgc attgtatgcc
     9961 cgtaaaaaag tgtttaacgg aggtggagtg tgagtgttgt tgtttcgggg acgctgaaat
    10021 ctcctgatgg tgaggcgata tcaggagcaa atattaccct gacggcgctg acagtttcac
    10081 cggatgcgct cagcggcacc agtgcgtcgg cagtgacccg tgaaggtgga tattacggaa
    10141 tgacgatgga tccgggggag tatgcggttt cggtgacggt gaaagggaag actgctgtct
    10201 acggacgtgt gcgtattgag gggaccgaaa gtacggtgac gctcaatatg ctgttacgcc
    10261 gcagtcttgt tgaggttagc atacccggag aactgctgac agatttccgg cagatacaga
    10321 ataatgtggc tgatgacctt gccactattc gtcgcctgaa tgaagacacg gcgacaaaaa
    10381 acactcaggc cacacagtca aaagaaagtg cagcagccag tgcgaagagt gcatctgaca
    10441 gtgcaaagac ggcaaccagc agggcggctg aagccggaca aaaagcgact gatgccactg
    10501 aggctgcgac ccgtgcagtc acagcagcgg ggaatgcaga ggaaagctcg acccgtgccg
    10561 gagagtctga aaaagccgcc ggagctgatg cagaaaaagc cagacagcat gctgaaaagg
    10621 ccaggctggc gcaggagagc gccggagaga tccttaagcg ggcagaggct gccactgtca
    10681 gtgctgaaga ggccagacgt atggctgaga atgcacgggg gccccggggg cctcagggag
    10741 aaactggtcc gaagggggat gtcggtccta aaggcgaaac aggtccagtg ggccctcaag
    10801 ggcccgcagg gccgaaaggt gagcgtggtg acgttggtgc tcagggggct gtagggcctg
    10861 ctggtccgcg tggtgagaag ggcgaacagg gggagcgagg accgcaggga ataccaggcc
    10921 tgaaggggga ta
//
LOCUS       DAZKIF010000139        10917 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZKIF010000139 DAZKIF010000000
VERSION     DAZKIF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.140"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..187
                     /locus_tag="ACHOTW_003908"
     CDS             <1..187
                     /locus_tag="ACHOTW_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845929.1"
                     /translation="VNALNAKIVDCDDKIRSKLTIVRNNRLENQYKDDKNISDAMRNI
                     LDWFERYPDIVQNITQA"
     gene            complement(324..989)
                     /gene="yieH"
                     /locus_tag="ACHOTW_003909"
     CDS             complement(324..989)
                     /gene="yieH"
                     /locus_tag="ACHOTW_003909"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP9845930.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            1155..2492
                     /gene="adeP"
                     /locus_tag="ACHOTW_003910"
     CDS             1155..2492
                     /gene="adeP"
                     /locus_tag="ACHOTW_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP9845931.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(2546..3112)
                     /gene="yieF"
                     /locus_tag="ACHOTW_003911"
                     /gene_synonym="chrR"
     CDS             complement(2546..3112)
                     /gene="yieF"
                     /locus_tag="ACHOTW_003911"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP9845932.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3134..3883)
                     /gene="yieE"
                     /locus_tag="ACHOTW_003912"
     CDS             complement(3134..3883)
                     /gene="yieE"
                     /locus_tag="ACHOTW_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP9845933.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            complement(4040..4999)
                     /gene="yidZ"
                     /locus_tag="ACHOTW_003913"
     CDS             complement(4040..4999)
                     /gene="yidZ"
                     /locus_tag="ACHOTW_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP9845934.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            complement(4974..6149)
                     /gene="mdtL"
                     /locus_tag="ACHOTW_003914"
     CDS             complement(4974..6149)
                     /gene="mdtL"
                     /locus_tag="ACHOTW_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP9845935.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            complement(6281..7528)
                     /gene="tnaB"
                     /locus_tag="ACHOTW_003915"
     CDS             complement(6281..7528)
                     /gene="tnaB"
                     /locus_tag="ACHOTW_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP9845936.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            complement(7619..9034)
                     /gene="tnaA"
                     /locus_tag="ACHOTW_003916"
     CDS             complement(7619..9034)
                     /gene="tnaA"
                     /locus_tag="ACHOTW_003916"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP9845937.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            complement(9255..9329)
                     /gene="tnaC"
                     /locus_tag="ACHOTW_003917"
     CDS             complement(9255..9329)
                     /gene="tnaC"
                     /locus_tag="ACHOTW_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP9845938.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            9729..>10917
                     /locus_tag="ACHOTW_003918"
     CDS             9729..>10917
                     /locus_tag="ACHOTW_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFP9845939.1"
                     /translation="MVDNVTVSRVCIQSPSFVPDLDGEKNKSQLFVDDIVAYLKSPSV
                     YSLEKEGPLNHFVNHCSEVELGFYSDGAYSILVSRSKQQPEGMILTVSDADAINIVHI
                     SVSPVLIKFLDDIFTCLHTYPDDESFTKEQIKANSKYDIVDYNCLLHFTGKPKSLIEC
                     RHFALQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFNNLQKNPPPGTCVVA
                     SDKFGEALSVFFHRMEKEKLTHMTAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPN
                     ATNTAVRYKANNCDSFGSLQSFINIQQAKQKWVITDICSECVGITPYLPREQAHLLSG
                     IENELQPPLSPPALFLLMRMGIYKNIVLFFDKLKNSQEMTASKALDILAAKSPEGIYG
                     LCVL"
BASE COUNT         2928 a         2577 c         2613 g         2799 t
ORIGIN      
        1 agtaaatgca ttgaatgcga aaattgtcga ttgtgatgat aaaatacgtt ctaagcttac
       61 tattgttcga aataatagac ttgagaatca atataaagac gataaaaaca tcagtgacgc
      121 gatgcgtaat attctggatt ggttcgaacg gtatcccgat attgtacaaa atataaccca
      181 ggcttaacca tacagagggt ggatgacagg acgaataacg acataaatta ttcataggga
      241 atagacgctt actcatcaga cacctggtgt tctgtatctc aaaccagcgt gactgatgtg
      301 taagtgttga agtaaggagg cgattatcgc gttatattcc agccgcgtgc cttccagagc
      361 gcaggcagct gcgacagctc ggtaaaggtg gtgactttcg ggtgaacgat cggcttattg
      421 tgcgggtcag cgcagaagta aaacacttcc attccggcat ctatccctga ttgagcgcca
      481 gcgctggagt catcgaccag aatgcagttt tcaacgttca cattcatcgc ttttgccgca
      541 tggaacatta atgccggatc gggcttccaa cgctgaatgt catatccgct gaacagtttt
      601 tccgggaagt agtgcagcat gttgagctta ccaagcgaat gctgcatttt gctgaccgga
      661 ccgttagaga cgacgcacat cggcacgtta atgctcgcca gcaattcgtt cgctcctgcg
      721 atgtcctcaa gttcggtatc aaacagacgt gctacttctg cgcggtaaac gtgctcggca
      781 tcggcctgga ccatcgtcac gccctgttct ttactgatga tatcgatgat ttcgtagagt
      841 tttacgcctt taaaacgggt aaaaatctct ttcaggtcaa cgtgaatgcc aaattccctg
      901 aacatcgtga cataggcgcg agagcaaatg acctcgctat cgaccagcgt accgtcgcag
      961 tcgaaaaata ccgcttctat ctgggacatg tctttccttt atcgttagtt taacgttttc
     1021 gtcatgccaa ttcgcatgac gcaatcgttg ccgtataagc aaatacaatg aaaaaagtta
     1081 tcttcagccg tccatattgt ctcattttgg tatacgatag cgacggattt tccctccttg
     1141 ttcggaaata gataatgagt caacaacaca caacccaggc ttctggccag gggatgctgg
     1201 aacgcgtgtt taaactgcgc gaacatggca cgacggcacg gaccgaagtg atcgccggtt
     1261 ttaccacctt cctgacgatg gtttacatcg tttttgttaa cccgcaaatt cttggcgttg
     1321 ctggcatgga taccagcgcc gtcttcgtca ctacctgtct gattgctgca ttcggcagta
     1381 ttatgatggg gctgtttgct aacctgccag ttgcactggc acccgctatg ggcctgaatg
     1441 cgttcttcgc ttttgtcgtt gtgcaggcga tgggcttgcc gtggcaagtc gggatgggcg
     1501 caatcttctg gggcgcgatt ggtctgctgt tactgacgat tttccgcgtt cgctactgga
     1561 tgattgccaa cattccggtg agtctgcgtg tgggcattac cagcggtatc ggtctgttca
     1621 ttggcatgat ggggctgaaa aacgcaggtg tgattgtcgc taacccggaa acgctggtca
     1681 gcatcggtaa tctgacttct cacagcgtgc tgctggggat cctcggcttc ttcatcattg
     1741 ctattctggc ctcacgcaac attcacgcag cggtgctggt ttctatcgtg gtgacaacgc
     1801 tgctgggctg gatgctgggt gatgtccact acaatggcat cgtttctgcg ccgccgagcg
     1861 taatgactgt cgtgggtcat gtagatttag ccgggtcgtt taacctcggg ctggcagggg
     1921 tgattttctc tttcatgctg gtcaacctgt ttgactcctc cggcacgttg attggcgtga
     1981 ccgataaagc cggtctggca gatgagaagg ggaaattccc gcgcatgaag caggcgctgt
     2041 atgtcgacag tatctcttcc gtgaccggtt cgtttatcgg tacttcttcc gttacggctt
     2101 atattgagtc ctcttccggc gtatcggttg gcggtcgtac cggtctgacg gcagtggttg
     2161 ttggtctgct gttcctgctg gttatctttc tgtcgccgct ggcgggaatg gtgccaggct
     2221 acgctgcagc tggcgcgctg atttacgttg gcgtgttgat gacctcaagt cttgctcgcg
     2281 tgaactggca ggatcttact gaatctgttc cggcgtttat taccgctgtg atgatgccgt
     2341 tcagcttctc gattaccgaa ggtattgcgc tgggctttat ctcctactgc gtgatgaaga
     2401 ttggtaccgg gcgtattcgt gaccttagcc cgtgcgtaat catcgttgcg ctgctgttta
     2461 tcctgaagat tgtatttatc gacgctcact aaaatcgata gcgcgttaaa aacccgccat
     2521 gcatgattgc tggcgggttt tttatttaga tcttaactcg ctgaataaac tcgccaaatg
     2581 cggtcaattg cccggtcagg tggtccagcg taccctgatc aatcacttct ccggtttgcg
     2641 gatcaacttt gttctgaatc acgccgccca taaattccgg cttgttcatc accattgcat
     2701 cgaggaaaac cagaatctgg cgcaggtgat actgacagcg cgcgccgcca atcacgccca
     2761 ttgagctggt ctgaatcaac accggtttac cggccagcgg ttgatccggc aggcgggaaa
     2821 gccagtcgat ggcatttttc agcccacccg gtaccgaata gttatattcc ggcgtgacga
     2881 tcaccacacc gtcagcctga cggatctgtt ccgccagagc ttcaaccgtt gctggaaaac
     2941 cttcttcctg ctgtacgtca gcgtcataca ggggaatgtc ggcaatggaa ggtaacgcat
     3001 tgacttccat gctcgccgga gcaattttcg gtagggtacg tgcaaccatg ccattaaatg
     3061 agcctttgcg caggctcccc agtaacgtaa ccacctgcaa tttttcagac ataatgactc
     3121 ctgtggttgt ctatcaattt ggcgaaaaag ccttcatggt agagagctgg gcaaaacgca
     3181 tcatccggct ggtaggattg ttggcgcggc tgtgaatatc cggcaggctt ttccagccgt
     3241 gtttgccatc taactcccag acactgagct tttcaatcgt gggcgtgacc gccacggacc
     3301 acaccagcac gtcctccgcg tcgcataacg cttctacatg atttacatga gcacatttca
     3361 ggcgtccggc aatgggcagc agttgcagat cgcgcggatc gtcattcaaa acatcaccgg
     3421 ttagttttag cacgcttcgg cgcagcgtga tgagctgcgc ccgcgcttcg tttggatcat
     3481 tttgtttact gacccatagc gattcattgc tggaaaatgt gtggttatcg ggcgcgtgtg
     3541 ggctatgaaa cccgcgcgtc gcacgctgca gttccatatc gaggccacat tcaccttcgg
     3601 ttgttaacgc cacgccaacc atattcccgg cataggaaat agaaaacgaa ggcaaatttt
     3661 tatcactgaa aaccggttta cctttcggca gggtgacgat ttccggcaat tcgccaatgc
     3721 catacagcat gaacatcagt tctgcgagta aacctctgga cgccagaaaa cggctttgac
     3781 gatgaggggg aatgtttcgg gcttcttgat gacactggga ggggagacga acagaaatca
     3841 gatgtccttc cgttaaaatc cctcgggcaa agtgagtcgc catttttcgc tccatgctta
     3901 atggtccatt atgaatacaa gagtaatcat ttactggaat gatggacaga ctaaagcggc
     3961 taaaagttcg attcagacaa gcttattaca acttttttac attttattgt gaaatcgggt
     4021 cgcgtttcat acgattcggt tatgccatcg acgcgtaaag atttttaatg gtatcccgta
     4081 accagacgat cttcggatta tggctgttcc gtttatgcca cagcagggta aaagggactt
     4141 ccagcttttt ttgctggctt tcgtcaaacg ggagaggaag agcaaccaac ggcagttggt
     4201 ggagttgatt gtagtactga cagtagcgcg gcgcggtcgc caggagcaga ttgtcgggct
     4261 gcgctgccat aaacagtgac tgctcgaatt ccggcaggct cattgcaatc gtacgttcgc
     4321 gccccagctc ctgtaacaca ttgtccagcg cccaggtatc gctctgttcc cagcaaatgc
     4381 tgatatgcgg ataacgtaaa aaggtatcga gatcccactt ttcatgcagt gccggatgat
     4441 ctttgcgtaa ccagacgcag ggcacatcac taaacaacac ttcataatca atggctaacg
     4501 gtagcgagct taacagctcc cgcgaacggg gatgactttc gcgaccggaa aaaccgatat
     4561 ccacttcacc acgagtaatg gcatctaagg aatcgtaatc ccagttacgt aatttgatgg
     4621 tcgcctgcgg gtaacgttgg tagatctgtt tcgacagcgc attaagcatg atcatcatta
     4681 gcggtgattc tgccgccagc tcaaacttca ggccgcgcgg tgtctggtgg tgcggtttat
     4741 ccagcagcag gttgctcatt tgcatccact ccgccagatt ttgctccatg ctgaccatta
     4801 gcggtgtggg cgacagcccc aacggtgagt tcacaaagag cgggtcgtca aaccacgctc
     4861 ttaactttgc cagcgactta ctcaccgccg aaggtgtcac gttcatccgc ttcgccgctt
     4921 tggttacgct gcgctcctgc atcagcagct gcagacagag cagcagattg agatcaagcg
     4981 tggtgatgga tttcttcatg agcggtaacc gggcgtccag gcgcgacgaa cataatcagc
     5041 aacaggctca ctatgctaca ggcaatcaga atcccgatca gcatattcca tgcgctgata
     5101 ccaaccaccg ctgccagcca aatccacagt gacgaaccgc aaacctgcgc aatacctaag
     5161 gtcgagctgg ctacgcccgc gcgtaatgaa aacggcccta acgcctgact catcgccaca
     5221 ccaaaaccta ccgagaaacc ggcgcaaatc agcgtgatac caaacagaga aaccgcatgg
     5281 gaaggtgaaa cggtaagagt gatgcccgcc gccaggaata acacctgtga ggtgatcatc
     5341 aacgtacgtg gcttaaaaat tcccagcgca aatggcgtgg aaaatgaaac ggtcatgctg
     5401 acgccagcgg tcaacgccat aatggtggcg tattctccgc gctcaaaacc catgatttcc
     5461 atcagcaata ccggcgatgt attgacgaag gtgaggatca ccgaaacgct gagggtggtg
     5521 ataacaacac ggctgaggaa aaaccgatta agcagcgact cgctattttc tcgtgatttg
     5581 tccgaagctg cgggggcggc agggcgcgtt tcttttaaaa taaacaaaga caacatcagt
     5641 accgcgatgc ccattattgc catcgtccag aacagactct gccacgggaa tttaagcata
     5701 atcagatgac cgagcaccgg cgctaacacc ggaatgatgc aggtaatgcc gttgagtaac
     5761 gacagcactt ttgcccgacg tcgatcatcc agcgtgtcgc gcaaaatggc gaatgccacc
     5821 acgtaacaac agcccgcgcc caacccctgt agaaagcgcc ctgcaagaaa taacgtgctg
     5881 gtttcagcca gtgaacagaa caccgaagtg ataataaata gcgccgcgcc ggggatggcg
     5941 accggttttc tccctgaacg atcggccact ttaccggcaa ataacatcgc agctgccata
     6001 cctgccagat aaacggagaa cgcaatatgt aactgcgctt cgctggcatt gagatcggcg
     6061 gcgatgcgcg gtaaaccaac gaggtacata tcaatcccag cgggataaag taaaaccagg
     6121 gcaaaactac aaatcaaaaa gcgggacatg ggctaaagcg tcctgatagt aaggtgacgc
     6181 aaagcatacg tggtgaagga ggatcaggcg agttgccatt tggacataag tgatttcctg
     6241 agaggcaaga agccagcgaa tgactggctt cttgaaggat ttagccaaat ttaggtaaca
     6301 cgttaaagac gttgccgaac cagcacaaaa ttacggttat accaaagaga atgacaatcg
     6361 ccggaataag attgccgccc cagaccgtga acatctgatt gggaaacttt ttgcgagctt
     6421 tgattgccag cactgcggga ataatgaccg cccaaatagt ggcgcacagt ccggcaccgc
     6481 cgatcccgta aataaagccg ttcgggaaga tcagatacaa caacgcgggt ggcaggaagg
     6541 ttaacagcac ggttttgaaa cgcccgccgt gggagttatc aatcttaaac aggtccgcca
     6601 gataatcgaa caatcccagc gtgacaccaa aaaacgagct ggcgacagct aagttggaga
     6661 acaccagcag gcaaaactcg ataatgccgt gctgtttggt gccgaggaac gatttcacca
     6721 gtgaatcaac gttgccgcct gaggaaataa tcgccttaaa gctttctcgc ggaatattcc
     6781 ccatggtgca atagagccag aagagataaa tcaccagcgc cagcagcgaa ccgaagacca
     6841 cgcttttgat tagcttatct ttgcgtttgc catagcaaat aatcaggctg ggaatattgc
     6901 cgtggaaacc aaatgacgcc agacacaccg gcaaagccat aaagatatac gggaagtaag
     6961 acgttcccgc agtggagctg gtggcatcgc gcagaatgga gtaatcgacc aggaagaaga
     7021 aagaaccaaa cacgatcaca aaggagataa tcttcagccc gaggaacaat gaggtaatac
     7081 gactggcggc taacgagctt atccacaata cgctggcaac gaaaatggct gtgcagatcc
     7141 cgacaatacg tggattagcg tgataaccca aattcattga tatcgtttca ctaatgatcg
     7201 caccattagc agagatatag gcataagtga ggatatagag aacgaaggca acggtaatac
     7261 cgctgataat gttccaggtg ttaccgatta aatctttggt gatggtgtta aaactggagc
     7321 cgacgggata atttaaattt gcttctaata acaataaccc ggaatgaagc attgaaaacc
     7381 aggcaatgat aaggataaag gcaccccaga aaaaccaggc accggcaaga tccacaggta
     7441 aagcaaacat acctccaccg attactgtac ctgctataac cataacaccc caaaatgcag
     7501 agtgcttttt ttcagcttga tcagtcatga tgccaccttt agaggaaggc tatttttgtt
     7561 attgaggatg tagggtaaga gagtggctaa catccttata gccactcagt agtattaatt
     7621 aaacttcttt cagttttgcg gtgaagtgac gcaatacttt tggttcgtag gtaaaggtta
     7681 accctttaat attcgacgcg ttctctttca catgtttgaa ggcttcaata atgaagtcca
     7741 tatgtgtttg agtatatgtt gcgcgcggaa tggttaaacg cagcagttca gccgggcatg
     7801 gcagttgttt accggttttc ggatcgcggc ctaacaggaa agagccaatt tctaccgcgc
     7861 ggataccggc gactttatac agctcgcagg ccagcgcctg tgccgggaac tgatctgccg
     7921 ggatatgcgg aagcagttta ccggcatcaa cgaatgccgc gtgaccgccc gcctgctggc
     7981 agacaacgcc aatctcttcc agaccatcga ccagatactg cacctgcgca atacgataag
     8041 ccagccagtc cagattcatg ccgtcataca gacctacggc cagacgctcc atcgcgccgc
     8101 cttccaggcc gccatacgtc gggaagcctt cctgaactac gcaaagggtt ctgcactcgg
     8161 tgtacacatc aaagaaactg tcgtctttca tgcacagcaa gccgcccatc ggcaccatcg
     8221 catctttctt ggcggacatc gccagcatat cggcatattt gtaggtttcg cgggtgatct
     8281 gctcgatggt ccagtctttg tattctgctt cacgctgctt gatgaaatag gcgttttcag
     8341 caaagcgcgc ggagtccatt accaccggaa tatcatattt cttcgcgatg ctgtacatcg
     8401 cttttaagtt tgccagtgaa accggctgac cgcctgcgga gttactggtg atggttgcaa
     8461 cgatatacgg cacgttattc ggaccaactt cttcaatacc gcgttctaat ccctcaaggt
     8521 caaagttgcc tttaaagtcg taacgcacgc ccgtatcgaa ggcttctttg atatagacgt
     8581 tacgcacggt acagccgtta atctggctat ggccctgcgt ggtatcaaag aaatagttag
     8641 agaacgccac cattttgctg cgatccaggc ctttttcctg ctcgcgcttt ttaatcagta
     8701 ctggaatata gatttgctct gcaccacggc cctggtgagt cggaatggtg tattgataac
     8761 caaagatatt tttcactgac tcggctaacg catagtagct gcggctgccg ctgtaagctt
     8821 cgtcgccgcg catcatcgcg gcctgcatac tctgcgtcac cgccccggtg ccgctgtcgg
     8881 tcagtaaatc gataaacaca tcttcgctat ccagcaggaa cgggttcata ccggatttaa
     8941 taattgcctc ttcacgataa gcgcgagtgg tacgttttac tggctcaata acacgaatgc
     9001 ggaacggttc agggagatgt ttaaagtttt ccattacata atccttcatt tattttaatt
     9061 acagtgatcc ctgtgaatat tgcatctgca atatccatta attcgccatg tatatatgca
     9121 agcaccatca tttactaaca caagtaaata aacgagagat ggcctttgca aaaggcaaat
     9181 ttaagatata gcaaacaaat agatcacatt gaatctaatc ggtttggctc tggtgatggc
     9241 tacagaaggg caaatcaagg gcggtgatcg acaatcttgt tgtcaatatt gaaccatttt
     9301 gaggtcacac atatatgtaa gatattcata atgcacttat cctcgaaaga cacgaagcta
     9361 cagtataata ctcaagaaat ttgtctatta tgaaattgtt taaatgtgaa tcgaatcaca
     9421 tttagttatt ggctgtgttt atgagaactg acacataaag aatattaaca aaaatacgtg
     9481 aatggataat ttttttaatt tttataatta ttgttgaaga tgatgatatt gattataggg
     9541 ataccattac aaggagctgt tcccctcgtt gatgtcgcgg tttacccacc tcattaccag
     9601 gttgatgacc tgatggggaa atgacgtaaa aagacaaata actctataaa tgagcgaaat
     9661 ttgatagttg atttttaaat agtaacctta ttattactac aactttcata tggatgaggg
     9721 gaaagaaaat ggttgataac gtaactgtta gcagagtatg tatacaatct ccttctttcg
     9781 tgcctgattt ggatggagaa aaaaataaat ctcaattatt cgttgacgat atagttgcgt
     9841 atcttaaaag tccttcagtt tattcacttg aaaaagaggg gccgttaaat cattttgtta
     9901 atcattgttc tgaagttgag ttaggtttct atagcgatgg tgcatattca attcttgtct
     9961 ccagatctaa gcaacaacct gaaggtatga ttttaaccgt aagcgatgca gacgcaatca
    10021 atatagtaca tatttccgta tctccagtgc ttataaaatt cctggatgat atttttactt
    10081 gccttcatac gtaccctgat gatgagagtt ttacaaaaga gcagataaaa gctaatagca
    10141 aatatgatat tgtagattat aattgcctgt tgcattttac tggaaaacca aaaagtttaa
    10201 tagaatgtag acattttgct ctgcaatact gtatagattc aatgaatgag catacaggga
    10261 aagttccatt aaaggcttac tattcatctc cggaagatat acaaaaacat attcctttcg
    10321 agcttgagca gcaatttaac aatctacaaa aaaatccacc acccggtaca tgcgtcgttg
    10381 ccagtgataa gtttggggag gcattatctg tcttttttca cagaatggaa aaagagaagt
    10441 taacgcatat gacggcaatc gttcaatctc aaacacacgc tatggccgtc cgcttgagga
    10501 tcaaaaaaac gcctgctggt gaaacagaat atgttgtatc cttttatgat cctaatgcaa
    10561 ccaatactgc agtacgctat aaagcaaaca actgtgattc ttttgggtca ttgcaatcgt
    10621 ttataaatat tcaacaggca aaacaaaaat gggtaataac agatatttgc tccgagtgtg
    10681 taggaataac cccttatctc cctcgggaac aagcccattt attaagtggt attgaaaatg
    10741 agttgcaacc tccattatca ccaccagcat tatttctatt aatgagaatg gggatatata
    10801 aaaacattgt tctttttttc gataaattaa aaaactctca agaaatgaca gcatcaaagg
    10861 ctcttgatat tcttgctgcg aaatcacctg aaggaatata tgggttatgt gtattat
//
LOCUS       DAZKIF010000140        10907 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZKIF010000140 DAZKIF010000000
VERSION     DAZKIF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.141"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            117..878
                     /gene="adiY"
                     /locus_tag="ACHOTW_003919"
     CDS             117..878
                     /gene="adiY"
                     /locus_tag="ACHOTW_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP9845940.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1015..2352
                     /gene="adiC"
                     /locus_tag="ACHOTW_003920"
     CDS             1015..2352
                     /gene="adiC"
                     /locus_tag="ACHOTW_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP9845941.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2456..4099
                     /gene="eptA"
                     /locus_tag="ACHOTW_003921"
     CDS             2456..4099
                     /gene="eptA"
                     /locus_tag="ACHOTW_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP9845942.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4096..4764
                     /gene="basR"
                     /locus_tag="ACHOTW_003922"
     CDS             4096..4764
                     /gene="basR"
                     /locus_tag="ACHOTW_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP9845943.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4765..5865
                     /gene="pmrB"
                     /locus_tag="ACHOTW_003923"
     CDS             4765..5865
                     /gene="pmrB"
                     /locus_tag="ACHOTW_003923"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP9845944.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5841..5930)
                     /gene="pmrR"
                     /locus_tag="ACHOTW_003924"
     CDS             complement(5841..5930)
                     /gene="pmrR"
                     /locus_tag="ACHOTW_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP9845945.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6042..7544)
                     /gene="proP"
                     /locus_tag="ACHOTW_003925"
     CDS             complement(6042..7544)
                     /gene="proP"
                     /locus_tag="ACHOTW_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP9845946.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7808..8686)
                     /gene="rdcB"
                     /locus_tag="ACHOTW_003926"
     CDS             complement(7808..8686)
                     /gene="rdcB"
                     /locus_tag="ACHOTW_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFP9845947.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8683..>10907)
                     /gene="crfC"
                     /locus_tag="ACHOTW_003927"
     CDS             complement(8683..>10907)
                     /gene="crfC"
                     /locus_tag="ACHOTW_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFP9845948.1"
                     /translation="TQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPW
                     SQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTA
                     LPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRALMQ
                     RIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFV
                     HLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEE
                     VREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSM
                     WGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLW
                     EDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLR
                     QNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQDDS
                     EPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSES
                     GFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPDWG
                     RGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCL
                     TELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY
                     "
BASE COUNT         2724 a         2860 c         2690 g         2633 t
ORIGIN      
        1 gtaaaactcg ttatttttat gcatgtttat attcatcata caattatata accatttccc
       61 ggtatcgctt tgctttagcg agaactggtc ttttattcgc actcaggagt acatgtatga
      121 ggatttgcag cgaccaacct tgtattgttt tattgactga aaaagatgtc tggataaggg
      181 ttaatgggaa agaacctatt agccttaaag ctaaccatat ggcgttatta aattgtgaaa
      241 ataatattat cgacgtctcc tctcttaaca acactttggt tgctcatatt agtcacgaca
      301 tcatcaaaga ttacctccgg tttctgaata aagatctctc gcaaatacca gtatggcaac
      361 gtagcgctac gcccattctc accctgccat gcctgacacc agacgtcttt cgcgttgccg
      421 cgcaacacag catgatgccc gcagaaactg agtcagaaaa ggaacgaaca cgcgcattat
      481 tattcacggt gctatcccgt tttctcgaca gtaaaaaatt cctttcatta atgatgtata
      541 tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat tgaaagcgat attcacaaag
      601 actggaatct tagtatggta gccagttgtt tatgtcttag cccaagtctg ttaaagaaaa
      661 agctgaaaag cgaaaacacc agttatagcc aaataatcac cacctgccgc atgcgttatg
      721 ccgtaaatga attaatgatg gacggtaaaa atatctctca ggtatcacag tcctgcggct
      781 acaacagtac gtcgtacttt atttctgtct ttaaagactt ctacggtatg acaccgctgc
      841 attatgttag tcagcacaga gaacgcactg tcgcctgatt tttaacctta acgaagagct
      901 atattaataa cggcatcagc gataacccgg tcgataataa ttcaactatc gaatgcaggc
      961 gtatgatatg acgtaattta ttgtcacgaa gctcgccttc gcaggagttt aattatgtct
     1021 tcggatgctg atgctcacaa agtgggctta atccccgtca ccctgatggt gtcggggaat
     1081 attatggggt caggtgtttt tctgttacct gcaaacctgg cctccaccgg cgggattgcc
     1141 atttatggat ggttggtgac gattatcggt gcgctggggc tctcgatggt atacgccaaa
     1201 atgtcgttcc tcgacccaag tcctggtggt tcttacgctt acgcccgccg ctgctttggc
     1261 ccgtttctcg gttatcaaac caacgtcctc tactggctgg cctgctggat cggcaatatc
     1321 gccatggtgg tcattggcgt aggatattta agttacttct tcccgattct gaaagatcca
     1381 ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct tcgtcctgct gaacattgtc
     1441 ggtccgaaaa tgatcacccg tgtgcaggcc gttgccaccg tgctggcgct gatccccatc
     1501 gtcgggattg ccgtatttgg ctggttctgg tttcgtggtg aaacctatat ggcggcatgg
     1561 aacgtcagcg gcctgggcac cttcggtgca attcaaagta cccttaacgt tacgctgtgg
     1621 tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg tggtgaaaaa cccgaaacgc
     1681 aacgtcccta tagccaccat tggtggggta ttgattgccg ccgtttgcta tgtgctctcc
     1741 accaccgcga ttatggggat gatccctaat gccgcactgc gcgtttctgc ctcgccattc
     1801 ggtgatgccg cacggatggc gttgggtgac accgccgggg ccatcgtttc cttctgcgca
     1861 gctgcgggtt gcttaggttc actgggcggc tggacattgc tggcgggtca aacggcgaaa
     1921 gcggctgccg atgacggact gttcccaccg atttttgccc gtgtaaataa agcgggtacg
     1981 ccagtggcgg ggctgattat cgtcggtatt ttgatgacca tcttccagct cagcagcatt
     2041 tcaccaaacg cgaccaaaga attcggtctg gtttcttccg tgtcggtcat ctttacactg
     2101 gtgccatatc tttacacctg tgcggcgtta ctgctgctcg gacacggtca ctttggtaaa
     2161 gcacgcccgg catatctggc ggttactacc attgccttcc tctactgcat ctgggccgtg
     2221 gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca ccctgatggt catcaccgcc
     2281 atgtatgcgc tgaattacaa ccggctgcat aaaaacccgt atcccttaga tgcaccaata
     2341 agcaaagatt aattcccctt aatccagcaa agataaaagc caaccttaag aacttaaggt
     2401 tggcttaatt ttgctttgcg agcatatgcg cactttgttc gatggaaaca ccgtgatgtt
     2461 gaagcgccta ctaaaaagac cctctttgaa tttactcgcc tggctattgt tggccgcttt
     2521 ttatatctct atctgcctga atattgcctt ttttaaacag gtgttgcagg cgctgccgct
     2581 ggactcgctg cataacgtac tggttttctt gtcgatgccg gtcgtagcct tcagcgtgat
     2641 taatattgtc ctgacactaa gctctttctt atggcttaat cgaccgctgg cctgcctgtt
     2701 tattctggtt ggcgcggctg cacaatattt cataatgact tacggcatcg tcatcgaccg
     2761 ctcgatgatt gccaatatta ttgataccac tccggcagaa agctatgcgc tgatgacacc
     2821 gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct gcgctgattg cctgctggat
     2881 taaaatcaaa cctgccacct cgcgtctgcg cagtgttctt ttccgtggag ccaatattct
     2941 ggtttctgta ctgctgattt tgctggtcgc cgcactattt tataaagact acgcctcgtt
     3001 gttccgcaac aacaaagagc tggtgaagtc cttaagcccc tctaacagca ttgttgccag
     3061 ctggtcatgg tactcccatc agcgactggc aaatctgccg ctggtgcgaa ttggtgaaga
     3121 cgcgcaccgc aacccattaa tgcagaacga aaaacgtaaa aatttgacca tcctgattgt
     3181 cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc tacccgcgtg aaactaaccc
     3241 gcggttggcg aaagataacg tggtctattt ccctaatacc gcatcttgcg gcacggcaac
     3301 ggcagtttca gtaccgtgca tgttctcgga tatgccgcga gagcactaca aagaagagct
     3361 ggcacagcac caggaaggcg tgctggatat cattcagcga gcgggcatca acgtgctgtg
     3421 gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc gtgcctcacc agaacgtcac
     3481 cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc tatgacgaag tgctgttcca
     3541 cgggctggaa gagtacatca ataacctgca gggtgatggc gtgattgtct tacacaccat
     3601 cggcagccac ggtccgacct attacaaccg ctatccgccg cagttcagga aatttacccc
     3661 aacctgcgac accaatgaga tccagacctg taccaaagag caactggtga acacttacga
     3721 caacacgctg gtttacgtcg actatattgt tgataaagcg attaatctgc tgaaagaaca
     3781 tcaggataaa tttaccacca gcctggttta tctttctgac cacggtgaat cgttaggtga
     3841 aaatggcatc tatctgcacg gtctgcctta tgccatcgcc ccggatagcc aaaaacaggt
     3901 gccgatgctg ctgtggctgt cggaggatta tcaaaaacgg tatcaggttg accagaactg
     3961 cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac aatttattct ccacgctatt
     4021 gggattaact ggcgttgaga cgaagtatta ccaggctgcg gatgatattc tgcaaacttg
     4081 caggagagtg agtgaatgaa aattctgatt gttgaagacg atacgctgtt attgcaggga
     4141 ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg gcgtgacaac cgcgcggatg
     4201 gcggaacaaa gccttgaggc aggtcattac agcctggtgg tactggattt agggttaccc
     4261 gacgaagatg gactgcattt tctcgcccgt atccggcaga aaaaatatac cctgccggta
     4321 ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg ccgggctgga tgtcggtgcc
     4381 gacgactatc tggtgaagcc ttttgcgctg gaagagttac atgcccgtat ccgcgccctg
     4441 ctacgacgcc ataataatca gggcgaaagt gagctgattg ttggcaatct gacgctgaac
     4501 atgggtcgcc gtcaggtatg gatgggcggt gaagagttga ttctgacgcc caaagaatat
     4561 gctctgctgt cacggttaat gctcaaagca ggcagtccgg tgcatcggga aattctctac
     4621 aacgacatct ataactggga caatgaaccc tcgaccaaca ccctggaagt gcatatccac
     4681 aatctgcgcg acaaagtggg caaagcccgt atccgcaccg tgcgcggctt tggctatatg
     4741 cttgtcgcga atgaggaaaa ctaattgaat ctgatgcgtt ttctgcgccg accaatatcg
     4801 ctgcgccaac ggctgatatt gaccatcggg gccattttgt tggtgtttga gctgatcagc
     4861 gtcttctggc tatggcatga aagtaccgag cagattcagc tgtttgagca ggcgctacgc
     4921 gacaatcgca acaacgatcg ccacatcatg cgtgagatcc gcgaggcggt cgccagcctg
     4981 attgtccccg gcgtctttat ggtcagcctg acgctattta tctgctatca ggcggtacgc
     5041 cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg aagcgcgcac cgccgacaac
     5101 ttaacgccca ttgccattca cagcgccacc ctcgaaatcg aagcggtggt ttcggcgttg
     5161 aacgatctgg tcagtcgcct gaccagcacg ctggataacg aaaggttatt taccgctgac
     5221 gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt tgcatctgga actgctggcg
     5281 aaaacgcatc acattgatgt agcaccgtta gtggcacggc ttgatcagat gatggagagc
     5341 gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat tttcttccgg taattatcaa
     5401 catgtaaaac tgctggaaga tgtcattctc ccctcgtatg acgaactcag taccatgctc
     5461 gaccagcgac agcaaaccct gctattgcca gagagcgccg ccgacatcac cgttcagggt
     5521 gatgcaaccc tgctgcggat gttattgcga aacctggtag aaaacgccca tcgttacagc
     5581 ccgcaaggca gcaacattat gattaagctg caagaagatg gcggagcggt catggccgtt
     5641 gaagatgaag gaccgggtat tgatgagagt aaatgcgggg agttgagtaa agcgtttgta
     5701 cgtatggaca gccgttatgg cgggattggt ctggggttaa gtattgtcag ccgcattaca
     5761 cagttgcatc acgggcagtt tttcctgcaa aaccggcaag agacttccgg cacgcgggcc
     5821 tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga tataaagaaa gctgctgacc
     5881 accagcacgc tgaacacggt agttaaactt tcataaacac ggtttttcat tgcactctcc
     5941 tccgttaacc tggaggagag tatgcgcgtc agagattaag tgaaccttaa gagttcaatt
     6001 ggcctgataa gacagcgtta catcaggcca tccgtttcag cttattcatc aattcgcgga
     6061 tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg caatctcgtg gtcaatatca
     6121 tcgattttct gctcgatatt atcgtaatgc tcgacgagaa tttccttcgc ttcctgtata
     6181 tctgacgccg ccggtgttgc acctttcaac ggacgatttg ccgtctcttt catggttacg
     6241 ccggtgatta aaccaaccac cgccactacc atcaggtaat aggctggcat catcagattc
     6301 tgcgagcttt cgaccagcca ggccgccagt gttggcgtca gaccggcaac cagcaccgaa
     6361 atattaaatg ccgccgccag cgcgctgtaa cggatatgcg tcgggaacat cgctggcaag
     6421 gtagaagcca taacgcccgt aaagcagtta aggatcaccg ccagcatcag taacccggca
     6481 aaaatcaggc cgatgacgtt actgttaatc agaataaacg ccgggatcgc caacacaaac
     6541 agtgcaacac taccaagtag cacaaacgga cgacggccaa aacggtcact cagcaagccc
     6601 atcaccggct ggacaaacag cataccgatc ataatggcga taataatcag caccccgtgg
     6661 tcttcggagt aatgcaggtt atgcgacaaa taactcggca tataggtcag caacatgtag
     6721 taagtcacgt tggtggcaat taccagacca atacatgtca acaggctgcg ccagtattta
     6781 gtggcaatct ctttaaacga gactttcggg ccatcctgca aaccttcgcg gtcgccctgt
     6841 tccagtttat caacatgctg ctggaacgcc ggagtttctt ccagcgcatg gcgcaggtaa
     6901 agcccgataa tccctaacgg cagagcaata aagaacggaa tacgccagcc ccagtcgagg
     6961 aagttcgctt cgccgacaat ggtcgaaatt aacaccacca cgcccgcacc cagcacaaac
     7021 ccggcaatag aaccgaagtc cagccagctg cccataaagc cacgtttacg gtcaggggag
     7081 tattccgcaa caaatatcga cgccccggta tattcaccgc cgaccgagaa accttgtgcc
     7141 atcttacaga tcaacagcag aatcggtgcc caaataccaa tcgtgtcgta ggacggtatt
     7201 aagccaatac agaacgtact gatcgacata atcacaatag tgatagcgag gatcttctgg
     7261 cgaccatatt tatcgcccaa cataccaaag aagagtccgc caagcggtcg aatcagaaag
     7321 ggaacggaga aagtggcaag tgcagcaacc atctgcacgc tggggtcagc ccccgggaaa
     7381 aaaactttac ctaatgcgta agcaacaaaa ccataaacac caaaatcaaa ccattccatt
     7441 gcattaccca gtgatgctgc ggtaatggct ttacgcagtt taccgtcatc aataatggtg
     7501 acatcacgaa gggtaatcgg ttttactttt ttccttttca gcatagcttt cctcgcagag
     7561 ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg gccgcaaaag gggagcggga
     7621 attgcaggat gcaatctctg taacaagcgt agcgggtttg cttacaccct ccggttaagg
     7681 aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt aatgaccttt ttaccctacc
     7741 agcgtttaaa tttgtgatca acttcacaca tggatttcgt ttaggactca ttgatgtaac
     7801 tgattattta caccaaattt acagagaaga tttcctcgag cattgccccc gccagacgct
     7861 gcgctgaaca gagtcgtggc atccccagcg cgacagtttg ccagcgttgg gtcactgacc
     7921 agcaaacggg ttgtttatct gccgtggtcc actttcccag ccaacttaaa aaatcagcat
     7981 gatccccgcc cgtcggaacg gtcgaagagg caaaccactg atggtaatag tgacgcgtgg
     8041 tcggggccgc attgatactc tctgccagat gttgtgtcgc tttcacgcgc aaatcaccca
     8101 gcaacaacgc caccgcttgc gggcttccca gacgacaggc atcgccaaat gccccccaac
     8161 gcaactcttc gagaacgata aaacagcgcc caggcaacga ccaggcgtta tacgccccag
     8221 cacgccagcg ggtaaaaatt tgctcgctct gttcacccgc ctgcaccagt aggcctcgtt
     8281 gcgttaacgc cttttccttc cagcgacaac gggctgaaaa ccactcatac aaggcggcaa
     8341 tttgctgggg gagctccggg aaagcagagt gctggcggtg atccattttt tgcagcagcg
     8401 ccagatgctg cgcgatctgc gtcagtgcca gatgaccggg atccagcatc gacgccagtt
     8461 tttccaccaa tgtcagacgc agactaagcg gcgcatttaa catcccgccg ttcgtccatg
     8521 tctgaagctg cacgcgcgca agaagttcct gcatcagtcg ttcacgaaac tgctgctgat
     8581 gggcttgtgg aaggcgggca tcatcgccct gcgccagatc cactaaaaag cggggataaa
     8641 ccgactccag cacgcgaccg gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt
     8701 gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg gtgtaaatcc agccgtgagt
     8761 catcacagtc tgctgcaatt gctgttgcat cagcctgacc accgattcat tttgttgacg
     8821 cagagccaga ctttcgcgta aacgcgtctg taattccgtc aaacatgaag cgaactcagc
     8881 gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc tgctctgcca gaactttagc
     8941 catttgttgg caataaagat cgacttctgc gcttaatgct cgttgcaaca cactgtaatc
     9001 aaccgtttct gtcggggatt tctcatttcc ccgtccccag tcaggctgat tcaaccagcg
     9061 cgaaaaagtc tcacgcacaa cgcctaatcg cgtgctctgc tcgtccgttg cttcctggcg
     9121 cgaaatgact tcactgaaca gctggcgagt gttgaagtgg ggaactacgc cgtgaaaaac
     9181 aggaaaatga aacccaggac gaaaccctga ctcgctcaat tccattttga cttgttgctc
     9241 aatggggcga ataacatcgg ttaacacccg gcaaagggtg gattcaagct cggcaaaacg
     9301 cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt aacagtgtct cacagcgggt
     9361 acgaatctcg cttaacggct ccgaatcatc ctgaaacaag gcggctaact gcaccttcag
     9421 cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc agggccagct cgatttcatg
     9481 tttaatctcg ccgctcacct gcgcctgatt gagttgcaat agctgcaaac tttcttcgat
     9541 ctggtggata ttttgccgca attgttcaca agcgacgttt aacccgtgcg cacgaaaatc
     9601 caggtattcc cgcgcctgct gcgcgtaatt caacagttta tgcgcggcag atcgcagagc
     9661 atacaacgag gcgttagcgt aagcggcatg aagcaacatc tgaattggct gggcgaacag
     9721 cgaatcttcc cacaactgat cggcagcatg acgaatatgt tcgaggtccg ccagatcggc
     9781 atgacgccag cgcctgccga gcgcggcatg ggcaaaatct tccacccagc gttgttgctc
     9841 tggcggtggt aacttaccgt tgttggctaa ctcatggcgc gcccgatttg ccaggtagcc
     9901 ccacatcgac gacaccggaa atatctgctg tggcgtaata cagcctttca tcagcgtccc
     9961 ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt
    10021 attgaccagc acatacagcg gcaccgattg ccccaccgcc aaaatcgcct cacggacctc
    10081 ttcatcggag atcgatttca gttgcgtata atccagcacc gccagtaccg ccgaggcgcg
    10141 tgccagctgc tggttaagca ttttttgcag atgcggttgc ccggcttcat ttggcccggg
    10201 ggtatccagt aacgtcaatt gaccgggata actttccagc cccgccagat ggacaaactc
    10261 cacttcaatc acgggaatat gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc
    10321 cagcgccttc gccagtcgca ccaaatcatt caaacttttc agacaatgaa aaataggctg
    10381 ggcacccaga taatattttt cgaaagcgac gccattttcg atccgctgca taagcgcacg
    10441 catatcttta tctatttcca gcacatcggt cagatgctta atatcgcaat cacgcaggcg
    10501 ctgttgtaat ttttgaatta aacaatcgat tggcgcgaca tgtgaaaaat gcagtaccgg
    10561 ttccttttgg ccgggcgtat ggcgaataag cgtcggcagc gcagtcattg ggcgattgcg
    10621 attaggcagt acctccgtac caacaatggc attaatggtg gttgatttcc ctgctttcat
    10681 ggtaccgaca atcgccagca ccatttccag tcgggaaatt ttacgcaact cattattcag
    10741 catcgcgtga cgctcggcga tattaggctg actccagggt aaagccagtt gtggcgcatc
    10801 gtctccgggt acagagagag gcattttttc cagtaactgc aactgttggc gagaaagctg
    10861 taacagccgt tcagcctcct gacttaactc atacagggtc tgtgtgt
//
LOCUS       DAZKIF010000141        10878 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZKIF010000141 DAZKIF010000000
VERSION     DAZKIF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.142"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(277..870)
                     /gene="tehB"
                     /locus_tag="ACHOTW_003928"
     CDS             complement(277..870)
                     /gene="tehB"
                     /locus_tag="ACHOTW_003928"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP9845949.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(867..1859)
                     /gene="tehA"
                     /locus_tag="ACHOTW_003929"
     CDS             complement(867..1859)
                     /gene="tehA"
                     /locus_tag="ACHOTW_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP9845950.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            1983..2963
                     /gene="ydcK"
                     /locus_tag="ACHOTW_003930"
     CDS             1983..2963
                     /gene="ydcK"
                     /locus_tag="ACHOTW_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP9845951.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2958..3494)
                     /gene="rimL"
                     /locus_tag="ACHOTW_003931"
                     /pseudo
     CDS             complement(2958..3494)
                     /gene="rimL"
                     /locus_tag="ACHOTW_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(3557..3781)
                     /gene="ydcH"
                     /locus_tag="ACHOTW_003932"
     CDS             complement(3557..3781)
                     /gene="ydcH"
                     /locus_tag="ACHOTW_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP9845952.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(3921..5576)
                     /gene="opgD"
                     /locus_tag="ACHOTW_003933"
                     /gene_synonym="mdoD"
     CDS             complement(3921..5576)
                     /gene="opgD"
                     /locus_tag="ACHOTW_003933"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP9845953.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5801..7144)
                     /gene="ydcJ"
                     /locus_tag="ACHOTW_003934"
     CDS             complement(5801..7144)
                     /gene="ydcJ"
                     /locus_tag="ACHOTW_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9845954.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7361..8284
                     /gene="ydcI"
                     /locus_tag="ACHOTW_003935"
     CDS             7361..8284
                     /gene="ydcI"
                     /locus_tag="ACHOTW_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9845955.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(8322..9962)
                     /gene="trg"
                     /locus_tag="ACHOTW_003936"
     CDS             complement(8322..9962)
                     /gene="trg"
                     /locus_tag="ACHOTW_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP9845956.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGEPVSFAPV"
     gene            10361..10510
                     /gene="hokB"
                     /locus_tag="ACHOTW_003937"
     CDS             10361..10510
                     /gene="hokB"
                     /locus_tag="ACHOTW_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP9845957.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            complement(10488..10556)
                     /gene="yncP"
                     /locus_tag="ACHOTW_003938"
     CDS             complement(10488..10556)
                     /gene="yncP"
                     /locus_tag="ACHOTW_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP9845958.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(10582..10755)
                     /locus_tag="ACHOTW_003939"
     CDS             complement(10582..10755)
                     /locus_tag="ACHOTW_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845959.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
BASE COUNT         2801 a         2757 c         2548 g         2772 t
ORIGIN      
        1 ctttttaagt tatagcaaaa agcgccgctt cgtgttgtgg caaaaaaagt gttggtgaaa
       61 ataaattttt ttagcccctt ttcagacgac atagtcaatg ccgcaaaagg ggctaagtca
      121 atcatcagta gttaaatttc agtaagttaa gctgctaaaa aacttcgact actgtgatta
      181 atttcacatc ccttcgataa aaaatatgct taccgaaaag aaggacagta aaccatctca
      241 gcgtggggaa ttttcacgcg ctttgttctt accgggtcat tttttacgtg ccagcatcgt
      301 ggcgaaacgc agtttaatac gattaccgtt ggcgtcggtg cggtgcagct cgccgacgtc
      361 ttcattgtat ttcaccatct cccagccttc gtaatatcga cgtaattctc cctctttgaa
      421 ggcaaacggg aagccgacgg tacatggata atcagcggta tccatcgccg ccacaatcag
      481 gttgtaaccg ccaggtttag tgcaacgttg catattggca atcagccctg ggatggtttt
      541 agcctcaagg aacatcagca ccacagtcga aagaataaaa tcgtactctc catcaaatgt
      601 gaggttattc agatcaacga cccgggtgtg taaattatcc agattttcaa tggatttaat
      661 gcgctcgacg ttggcgatac tcatggcatt tttatcccat gcgtcaacat cataaccatt
      721 ggctgccagg taaagactgt tacgaccatt gccacagccc agatccagcg ttttacccgg
      781 tttaaccact ttcaccgctt ccagtacttc agagtgtgtg cgggttaatt catatttatc
      841 agtaaaataa ttttcgtcac gaatgatcat tctttgtcct ctgctttcat taaaacggcg
      901 cgctcggttc tgactaacaa ttttccctgc atcagaagcg ccaaagtacg gatgagcagt
      961 attgcaataa taaaattggt aaagataaac agcggcaccg ccagcgtatg gaaaaatcca
     1021 ttatcgctgc cactccccag atgcaaaccg gtggttgcca gtgcagatac gccgaacgag
     1081 aaactccaga atgaagcatt aaatggctgg gagagatacc atggcatcag acgtagcata
     1141 aacagcagtt gcagcagtcc ataaccgaaa agcattttcg ccagcgtgtc accctcgccg
     1201 ccgttgacgc tcagccaggc actacaggcc accagcgcag gagcgagctg aatgccgagt
     1261 gatgtccgca gtgccgtggg taattctccc gaactacgca gacgctgcaa gatcaccggt
     1321 tcaaggctta gccatgagaa aacgcctgcg cctaaaaaca ccagaccggc gtcggtgtag
     1381 cccaacgcac cacaggccat tgcgctgata aagttgttgg caactgtcgg cagatacagt
     1441 ccaggcgtag tagcttcttc agggtgagat ccgcgccata atcccgcagt ttgccaggcg
     1501 gcataagcca actgaaccac gacaccaaaa ctgaacaggc acaccgccag tgggcgaaac
     1561 cacggaacaa aaccaatcgc caccagcatc gtcgttgcag gaaacaaact cacaaaactg
     1621 ctcagcactg gatggcgaac ttccgccagc acgctatgcg gaaagcgtat gagtcgggta
     1681 ataaatgcgc tagtcaataa tccccagatg atcatcgcca gaatcaccag cccatcccct
     1741 aaccagtggc tgacctgcca aacctggctg gcatagcgcc aggcaaatcc catccctatc
     1801 gtccccaaca caataccaaa gtagcctgcc ggcaaattga gcactttatc gctctgcatc
     1861 ttcttactca ttttgtttaa tttataaagt atatttgaaa tgcattttat ggaattttct
     1921 cgagcatagc cagagccgca gaatttgcta cggttatagt aagtcacctg tgggaaaacg
     1981 ctatgcgcaa atatcgtcta agtgaagaac agcgagcctt tagttatcag gaagatggca
     2041 ctaaaaaaaa tgtgttatta cggcagatta tcgccattag cgattttaac gatgtgatag
     2101 caggaaccgc tggcggctgg atcgatcgcg aaacggtgtt agcacaggaa ggaaattgct
     2161 ggatttacga ccagaatgct attgcgttcg gcggaacggt gatttccggt aatacgcgca
     2221 tcaccggcac cagcgtactg tggggagagg tttacgcaac ggataatgtc tggatcgata
     2281 atagtgaaat caaccagggt gcatatataa gtgacagtgt caccattcat gactcactgg
     2341 tttgcgggca atgtagaata tttggtcacg ccttaattga tcaacattct atgatcgtcg
     2401 ccgcacaagg cttaacgccc gaccatcaac tcctgctcca aatttatgat cgcgcaaggg
     2461 ttagcgcctc gcgtattgtc caccaggcac aaatttatgg cgatgccgtc atccggtatg
     2521 cttttattga acatcgcgcc gaagtttttg attttgccag tattgaaggt aatgaagaga
     2581 acaacgtctg gctatgcgat tgcgctaaag tctacggtca cgcgcaggtg aaagctggca
     2641 tagaagaaga tgccattcca acgattcatt acagttcgca ggtggcggaa tatgccattg
     2701 tggaaggtaa ttgtgtgtta aaacatcatg tcttgattgg cggaaatgcc gtggtacgtg
     2761 gcggaccgat tctgctcgac gagcacgttg taatacaagg tgagagccgt attacaggtg
     2821 cggtgataat tgaaaaccat gtggagttaa ccgaccacgc cgtcgttgaa gcctttgatg
     2881 gcgacacggt tcatgtccgt ggtccaaaag tcattaacgg cgaggagcgc attacgcgaa
     2941 cgccgctggc agggttatta tgaatcgata atacgcgcgt ataagttcac atcatcatag
     3001 gcatcattca ggaactcagc ctgtttcagg caaccttcaa ggataaaacc attgcgcaaa
     3061 gcgacctggt tgctttgcgg attgtccacc cgacatttga tcacgaagcg tctaagttca
     3121 ccagactgag cgtaatgatg aatcagtgcc tgcagcgcct gagaaatgat cccctgcccc
     3181 tgatgagatt cgtccagtca gtagccgatt tcagcggttt tattcagtgg ttcaatacga
     3241 ttaaacgaga taacgccgat aagttcatct tctttgaaaa tcatgaacat tttggcatag
     3301 ccgcgttgat gcaacatcac attaccctgc accgtttttc gcgtgtcctc ttcaatctga
     3361 acgaactgcg gccagtttag cgactgctgt aaccaggttt tatttttaca gattaactga
     3421 taaagaggag tgacgtgatt ttctgcaaca gcatgtaatt caagtgattc gcttactttt
     3481 atcgtttcag tcatcttatc tccagtcaat ccatgcagtt gttaaaaaag ccgcctggct
     3541 ggcggcttag gaaagtttac acctctttga cgctctcatg ctgcaggatt ttgagcatct
     3601 catcttttaa ctgtagcttt tgttttttca tgcggaccac ttccgcattg taccctcgat
     3661 cgtcggaacc ttcctttctg gcaatttcat gatcaagttt attgtgttta tcgaacaagg
     3721 acataaagcg aggattttcg tttttcagac gggatattaa atctcgatat tctggaaaca
     3781 tacgcactcc ctgttagtgg ctgacaaggt gtatctgaaa tacacaccat cagattactc
     3841 atttaactta gggaaaaatg cgagcgggat cgtgtttaac cagacggcac ctcccgaaga
     3901 aggtgccgaa gaaaaaacga ttaactcatc acgcggtcgt caacatactg acgtttatcc
     3961 ggcgctggcg ggaaatactg atacagccat gtttcactga tagcgtcgcc ctgacaacgc
     4021 agatacatcc gcatatcgac cggatcagtg gagtccgaag tcggatacca gtcaaactga
     4081 atacgataac catcaatggg ttcaatatag agaatttcga tttgcttcgc ttccccactg
     4141 gaaagcgtaa tcaccggctc aatgcctttt ggtgcggcag ctttcagatc accaccaacg
     4201 aaatcgacgg caaaacgacg cgcccatttt tcgggatagt gttcacctgg tgcccaacct
     4261 tccgggaaac cgcccatgcc ggtacgcgtc gccataacgc gcgctaatgg gcaatgaaca
     4321 ggcggttgcg cactccagta cagacgatac tggaatgcaa actcatcacc cgcctttaca
     4381 gctttttctg gctgccagaa gcagacaatg ttatccagcg tttcgcccgt tgttgggatt
     4441 tccatcaggc cgatggtgcc cttaccccac ttgttacgcg gttccaccca cagacttggg
     4501 cgtttgttat accagcccat aatgtcctga taatgggaga aatcacgatc cagttgcaat
     4561 aaaccaaacc ctttcgggtt gttgtcggtg taagcattga actgcaattt ttgcggatta
     4621 ttcagcggac ggcaaatcca ctcgccgttg ccccgccaca tggacagacg atcagagtca
     4681 tgaatttgcg gatgaatggt gtcgcacatc cgacgttcat tagtaccgca gctgaacata
     4741 ctggtcatcg gcgcaatgcc cagctgttta atgtctttgc gcgcatacag gtgattttcc
     4801 acatccataa tcacctgact tttctcacaa tggatagtga acttataggc accagtaatg
     4861 ctggcgctat cgagcaacgc ataaacggta aaggtagttg cccccggttt taccgtatca
     4921 aaccagaagg cggtaaagtc ggggaactct tctttactgt cggtgtaagt gtcaatcgcc
     4981 aggccgcggg ccgacaaacc gtattgatat gtgtcatcaa cggcgcggaa ataactcgct
     5041 ccgaggaatg atactacatc acggcgcgcc agttcggggg ctttaaacac gcgaaaaccg
     5101 gcaaagccga gatcgctttg cccttctaat tgtttggtat caacacctgc atcgttgtat
     5161 ttgaacaact ccgggcgaaa gtgaatttca cgcgccagat gtgttgctgg atctacagaa
     5221 aacatacgaa cgcggcgacg gaatcccatt cccatatgga agaactgagc gtccagttga
     5281 cggttctcaa cgttatgcca gagcgatttt tcggcgtcgt attgaatact gttataagcc
     5341 tgcggcgtca ttgtcgccag cgtgtcaggt aacggacgag gcgcaccacg ccacgctgtt
     5401 tgcgctaagt cgtgcgccat tgactgtaga atggagaagt caaaacgctg ggtttgcccg
     5461 tcggcaatat cagaatctgc cgcgaatgcc gcctgagaaa aaagagaagc aatgccgctg
     5521 gtaccgcaca cggcggccat agccattgaa cctttaataa atcgtctacg atccatacct
     5581 gaaagtgagt ccttctggtg ttgtgaatgg tcccgcgctg gtcgttttca gcggcaagaa
     5641 agtgcttatt tatgaccgct cactctagac aatatttatt aataatccaa ttgttgaact
     5701 gagagaatat gcataaatcg gcaatttttt tatgtcgatg ggatgagcct gattcgtttg
     5761 taaaatcagg caaatttttg caaaggcgga tggtcagaat ttaaagcaaa ccacagcgac
     5821 gtttactgcg ttcttccgct tcctgataaa gctggaactc atccaaaacc ggacaaccca
     5881 acgcctgctc aaatgcttcg cgactggcat taccgtggct gcgtgcctgc gtttcattac
     5941 ccagatttga ctggaagatc cccgccgcgc taacgggcaa gaaatcttca taggtaatgg
     6001 gttgcgccgc gacccaacca cgttcaatta agggctgtgg atcgtctccg ggatgaatcg
     6061 cctgacgatg cgcctcacct gaaggcgtca gacggtaccg gaaccatgcc agcccttgct
     6121 gacgcattaa aaactcactg tcaggaaaag tgcggaaggt ttcctgtaaa tgcatttggt
     6181 gagtgagatt atcctgcccg gttccagcgt tacgcagaag atcatcatac agttgtcgac
     6241 ctttcggcgt taatgccacg ccacgctgct caatttcacc aaagcgcgca gtatgcgtgc
     6301 cctgtttctg ccccgcaaac aacaccgtct cttccagtgc tttaaagctg gtctggcgta
     6361 gtaaaatcgg tacctcgcgg cgcggcggcc cttcgatcag aatttttggt tcaattccgc
     6421 attcaggcat catcgactgc acccggtcaa tatccagcgt acgtggcgtc agatggttga
     6481 tatggcatcc aggaaaacag accacatcag caatcaaccg atgttcgttg tgcaaggcgc
     6541 gataggtttc ttcatctacc gttgctgact ggtgccaacg gaacgtttcc agggcctcct
     6601 gcacaaactc ctgtgcctgt gtttcgttaa aaccgccccg ctggtcatac tcctctaaca
     6661 gttgtcgaca acgtggggtg aagatatcgc gctgacgcag aatctccgcc gctttctggc
     6721 gcaaaatttc gttctcgata agctcaaggc ggagtaagga ggtaaaaacg cggaagggat
     6781 tacgcgccag agaagcatca tcaatgggcc gaaatgctgt cgaatgtacc ggcacccctg
     6841 cctgcgagag atcgtaatag ctcaccgggt acatccccat aatggcaaac atccgccgaa
     6901 gagtagcaag ctcttgtgca gtcccaacgc gaatcgcccc atgacgttca acattcagtc
     6961 gcgccagctc gtctgcattt accatttttt cgtgcagttg aggattgttt tccagcacag
     7021 ccagattcac atcagctacc agttccagca gcgtgccata ttgcggaact tcttgctggt
     7081 acatggctga cattgcctgc gaaaactgtt cccgaatctc atccgccgtg atgctgttcg
     7141 ccatagtgtc tgcctccagt ggtcagtaat ctggagtgta ggtaaccgca ttcactctgg
     7201 cgggaagaat ttacaaactg tgatctcgcc gcgaaaacat caatattatc cattttgctg
     7261 taacataatt gctttaattg ttaataatat tttgcaatca agttatcata atcaaacaac
     7321 ttcacttgtc agcgacaccg cttcgttttt aacatcgctt atggaaaaaa atagtctgtt
     7381 tagtcagcgc atccgtttgc gccaccttca tacattcgta gctgtcgcac aacaaggaac
     7441 tttggggcgc gcggctgaaa cccttaattt gagtcaacct gcgctctcta agacattaaa
     7501 tgaactggag cagctgacgg gcgctcgctt gtttgagcgt ggtcgtcagg gggcgcaact
     7561 taccttaccc ggcgaacaat ttttaacgca tgcagtcaga gtacttgacg ccatcaacac
     7621 tgccggacag tcgcttcatc gtaaagaagg tcctaataat gatgtcgtca gggttggtgc
     7681 actacctact gcggcactgg ggatattacc ttcggttata ggtcagtttc atcagcaaca
     7741 aaaagagacg accttgcaag ttgcgacaat gagtaaccct atgattctgg cgggtttgaa
     7801 aaccggggaa atcgatatcg gcattggtcg gatgtcagat cctgaactga tgaccgggct
     7861 taattacgaa ctgctgtttc ttgaatcgct gaagctggtt gtccgcccta atcatccgct
     7921 acttcaggag aacgtaacgc taagccgggt gctggaatgg ccggtcgttg tatcaccaga
     7981 aggcactgcg ccacgccagc attcagatgc attagtacag agccagggat gtaaaattcc
     8041 ttcgggttgt atcgaaacgc tgtctgcttc gctatctcgt caacttacgg ttgaatacga
     8101 ctacgtgtgg tttgtccctt ctggcgcggt aaaagacgac ctgcgtcatg ccacgctggt
     8161 ggccctgcct gttccgggac atggtgcagg cgaaccgatt ggaatactga cccgcgtaga
     8221 tgcgacgttc tcttctggtt gccagttgat gattaacgct attcgaaaat caatgccgtt
     8281 ctgaaaggtg aagggatctg tcgatccctc ctttaacatt ttcacaccgg agcgaaacta
     8341 actggttcac ccgctccgcg aggttctgcc aacacagatt gtttgttcag acggaatacg
     8401 tccaccgcct cagttaatcg tgccgcctgt tcttcaaggg acaccgccgc cgctgaggcc
     8461 tcttctacca gagaagcatt ctgttgcgtc accttatcca tttcagaaat cgcctggcta
     8521 acctgcgtta tgcccctact ttgctcatcc gaggcggcgg caatttcctg catgatatgt
     8581 gtgacactcg cgacggcatc aacaatagtg ctcatggttt tcccggccgt tgccacctca
     8641 tccgatccca ggtcaattaa cctgactgat tcactgatca agccttcaat ttcttttgcc
     8701 gcctgggcgc tgcggcttgc gagtgtccgt acttcgctgg cgacaacggc aaatccacgc
     8761 ccttgctcac ccgctcgcgc agcttcaacg gcagcattca gtgccagaat attcgtctgg
     8821 aaagcaatac tgttgatgac ggcggtgatc tcagaaattt tcttcgaact cgtggagata
     8881 gcgcccatcg tttttactac accggaaacc gtctgcccgc catcgctggc tttaatagaa
     8941 gcctcttgcg ccagtttgct ggcataatgc gcgttatccg cattctgttt caccgtcgca
     9001 gtgagttgct ccatgctggc ggcagtttgt tcgatagccg ccgcttgttc ttcggtgcga
     9061 gatgacaggt ccgcattgcc agctgaaatt tcgctggtgc cacgataaat ctcttccgca
     9121 ccctgtcgaa cagtccctac ggtcatcccc agtgaatgct gcatttgctg taaatgacga
     9181 cttaagcgac cgatttcatt acgacccgcc ggttcatcct tcatcgtcag atcgccacta
     9241 gcgatttttt caatccgttg tgcggcatgt tgcagtggac gaatgacgat ccgacgtagc
     9301 accataaatg ttatcagcgt catgaccagg gcaagcacaa acgcaccaat catgaacatc
     9361 ccgcccaggc gggtgcgctg atgggccagt tccgccagtt ggttggctct ggtgctacgt
     9421 atcttaacgg ctttgttcaa aatatcggta taagcattat ccagcgttcg gatctgctca
     9481 ctttcatgat tgataatcgc ttcaaacatg ccatttttgg catatttcaa cataggttgc
     9541 atacccgtga tataagcctg aaagcgttga tttaattcag tgtcgagggc ttcatcagca
     9601 ggtgttttca ccggccgatt ctgataagca cgataacctt gctgcgactg tttaatctcc
     9661 gattcggctt gcgcaatatt tcgcttcatt gcttccattt ccgcaatacg actcgcagcc
     9721 ccggcctgaa tcatattgat ccgggctgaa cgcaaaaaat ctgaactgtt cgctaacccg
     9781 gtccgaatct caatctccgc agtaacatca cgctgatcgc gatcggcctg ccagaggaaa
     9841 tagccagcca gggctgaact taatgcgaat agaactaaga taccgcctag aatgcaggca
     9901 aataacggaa ccaacctgat gtgatgcaaa aaacctaatc gctgtgaggg agttgtattc
     9961 attaccgttg tctctcgtcc aggtttactc ctgatagaaa gtcatcggca tttgacggta
    10021 attacttacg actaaaagcc tctttgcgtc acatttttac aacaattatt gcagagaaga
    10081 ttaatgccat tggaatgaag agagtaacga atggttaaac tggctaaccc gaatgacgaa
    10141 aaattctgga aaacagcacg caatttcgtc aggtgattat tttcgtccct aaggaaacca
    10201 cataggatgc tcacgggtgc ctgagacttt ctgttttcag gctaaccggg aatcagaaag
    10261 acgaaggccc cgaccgatat ttctatcaac cgaggccaac gaatgaacca agcaattcag
    10321 atgttagcct cttacccgcc atccggcaag gagaaaggct atgaagcgca accctctggt
    10381 ggtgtgtctg ctcattatct gcattacgat tctgacattc acactcctga cccgacaaac
    10441 gctctacgaa ctgcggttcc gggacagtga taaggaggtt gctgcgctca tggcctgcac
    10501 gtccaggtaa gggcaagcgc ggggattttc cccgcgcatc tgactagcag attcattttc
    10561 tcatggcacc ccttttacac ttcagttagt gcatgttttt ttcgatgcac taatagaacc
    10621 atcattacag acaaatttgc cgcctgccgt acagtgtgag atcccccctt tcgaaccaga
    10681 acagggtttg cgcccggcat cggcataaaa ggaaacaagc gctcccataa acaaaataag
    10741 tgccagtttt ttcatataac ctccatggtt tatcgaatct tatgttcgat ttgaacgtta
    10801 tacgctggtt tattttgata ccgttgatga gatcaaagat cgggtgaatt gattaaaaat
    10861 aagccagatg gacgtatg
//
LOCUS       DAZKIF010000142        10729 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZKIF010000142 DAZKIF010000000
VERSION     DAZKIF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.143"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..845
                     /locus_tag="ACHOTW_003940"
     CDS             <1..845
                     /locus_tag="ACHOTW_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HFP9845960.1"
                     /translation="YHEKAVMNYMEYLVTCQLDEEQMSSLLYERDDLSAIDFSLLMKT
                     IKAFSFGGDLQTLASKPSSTISSIPSKRRILININHDFPNNGNLFNDFLFNHQQDEQL
                     AMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFH
                     ALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGAWPNK
                     LQPGKYKNMGSNSVNIIINNTRKIIPPGKAFMLRGGTLNINIPGRSALLLGKTGEPLN
                     YLYL"
     gene            1182..2159
                     /gene="glaH"
                     /locus_tag="ACHOTW_003941"
     CDS             1182..2159
                     /gene="glaH"
                     /locus_tag="ACHOTW_003941"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP9845961.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2179..3447
                     /gene="lhgO"
                     /locus_tag="ACHOTW_003942"
     CDS             2179..3447
                     /gene="lhgO"
                     /locus_tag="ACHOTW_003942"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP9845962.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            3470..4918
                     /gene="gabD"
                     /locus_tag="ACHOTW_003943"
     CDS             3470..4918
                     /gene="gabD"
                     /locus_tag="ACHOTW_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9845963.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4932..6212
                     /gene="gabT"
                     /locus_tag="ACHOTW_003944"
     CDS             4932..6212
                     /gene="gabT"
                     /locus_tag="ACHOTW_003944"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP9845964.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGAHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            6450..7850
                     /gene="gabP"
                     /locus_tag="ACHOTW_003945"
     CDS             6450..7850
                     /gene="gabP"
                     /locus_tag="ACHOTW_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP9845965.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7871..8533
                     /gene="csiR"
                     /locus_tag="ACHOTW_003946"
     CDS             7871..8533
                     /gene="csiR"
                     /locus_tag="ACHOTW_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP9845966.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(8534..8983)
                     /gene="kbp"
                     /locus_tag="ACHOTW_003947"
     CDS             complement(8534..8983)
                     /gene="kbp"
                     /locus_tag="ACHOTW_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP9845967.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9067..9225)
                     /gene="yqaE"
                     /locus_tag="ACHOTW_003948"
     CDS             complement(9067..9225)
                     /gene="yqaE"
                     /locus_tag="ACHOTW_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP9845968.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            9408..9707
                     /gene="ygaV"
                     /locus_tag="ACHOTW_003949"
     CDS             9408..9707
                     /gene="ygaV"
                     /locus_tag="ACHOTW_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9845969.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9717..10235
                     /gene="ygaP"
                     /locus_tag="ACHOTW_003950"
     CDS             9717..10235
                     /gene="ygaP"
                     /locus_tag="ACHOTW_003950"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP9845970.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(10288..10692)
                     /gene="stpA"
                     /locus_tag="ACHOTW_003951"
     CDS             complement(10288..10692)
                     /gene="stpA"
                     /locus_tag="ACHOTW_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP9845971.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRIVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2535 a         2696 c         2876 g         2622 t
ORIGIN      
        1 aatatcatga aaaagccgtt atgaattaca tggaatatct ggtaacttgc cagttggatg
       61 aagaacaaat gtcatcgctg ctttatgaaa gagatgattt aagcgccatt gatttttcat
      121 tgctcatgaa gacgataaaa gctttttcat ttggtggcga tctccaaacc cttgcatcaa
      181 aaccgagttc cacaatctca agcatcccat caaaaagacg gatattgatt aacattaacc
      241 acgattttcc taacaatggc aatcttttca atgactttct atttaaccat caacaagatg
      301 aacaattagc aatggcatat atggccgctc tcccgttcag caggccttta gtttactggg
      361 atggccaagt attaaaatca acgactgaaa ttaaaaatta tgatgggtcg acgcgtgtcg
      421 gcggtgaggc gtggcttaat aaaggttgct ctacctatca gcagctctac aatgaattcc
      481 acgcattata tatagataaa gcaggaatat ggagcgcatt tgagggtgta tttgcaacta
      541 aaaacgttct ggcctttagt cgtggggatt cggtgaacat taatcactct cctcatgatg
      601 gactagttat aataaataaa ggaaacgaag aagttgaagg tgcctggcct aacaaattgc
      661 aacctggaaa atataaaaac atggggagta atagcgttaa cattattatt aataataccc
      721 gaaaaattat cccccctggt aaagcattta tgcttagagg cggaactcta aatatcaata
      781 ttcctgggcg tagcgctctt cttttaggga aaacaggaga accgctgaac tatctctatt
      841 tgtaatttaa tgttatatct gccccgctaa aacggggcag ataatatatt tagtttacta
      901 gcggtcattt tgcagtgaag ccatttaccg ttttttatcg accagataat ctgttctctg
      961 atggtaatcc ccccctaacc tgttgcttta gttattcatt tcctgtctca ctttgcctta
     1021 ataccctacg ttaaatgtta ccaatttgtt gctttttatc acaataagaa aacaatatgg
     1081 cgcatttgtg cgcatttttc agaaatgtag atatttttag attatggcta cgaaatgagc
     1141 atcgccatgt caccctacat ctcataagag gatcgcttct gatgaatgca ctgaccgccg
     1201 tacaaaataa cgctgtcgat tcagaccagg actatagcgg attcactctc atcccgtcgg
     1261 cgcaatcccc gcgtctgctg gaactcacct tcaccgaaca gacgaccaaa cagtttctcg
     1321 aacaggttgc cgaatggccc gtgcaggcgc tggagtacaa atcgtttctg cgttttcggg
     1381 taggtaaaat tcttgacgat ctgtgtgcga accagctgca accattgctg ttgaagaccc
     1441 tgctaaaccg tgctgaaggt gcgctgttga tcaatgcggt gggtgtcgat gatgtgaagc
     1501 aagcggatga gatggtgaag ctggcgacgg cggtggcgca tctgattggc cgttccaatt
     1561 tcgatgcgat gagcggtcag tattacgcgc gttttgtagt gaaaaatgtc gataactcag
     1621 acagctatct gcgtcagccg caccgcgtaa tggagctgca caacgacggc acctacgtcg
     1681 aagagatcac tgactatgtg ctgatgatga aaatcgacga gcagaacatg cagggtggaa
     1741 attcgttgct gctgcatctc gatgactggg aacatctgga ccactatttc cgccatccaa
     1801 tggcgcgtcg cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt
     1861 tccatccagt gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat atcgaccagt
     1921 tcgtccagcc aaaagacttc gaagaaggcg tgtggttgag cgagctttcg gacgccattg
     1981 aaatcagtaa aggcattctt tctgtacccg ttcccgttgg caaattcctg ttgattaaca
     2041 acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg cgccgtgaac
     2101 tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctatcagacg catcagtaag
     2161 cgcaaaggaa ttgagcggat gtatgatttt gtgattattg gcggcggcat catcggcatg
     2221 tcgaccgcca tgcaactgat tgatgtctat ccggatgccc gcattgcgtt gctggaaaaa
     2281 gagtccggcc cggcctgtca tcagacgggc cacaacagcg gcgtgatcca tgccggggtt
     2341 tattacacgc ccggtagcct gaaggcgcag ttttgcctgg cgggaaaccg cgccactaaa
     2401 gccttttgcg atcaaaacgg cattcgctat gacaactgcg gcaagatgct ggtcgccacc
     2461 tctgaactcg aaatggaacg gatgcgcgcg ttgtgggaac gcacggcggc aaacggtatc
     2521 gagcgcgagt ggttaaacgc cgatgaactg cgcgagcgcg aaccgaatat caccgggctt
     2581 ggcggtattt ttgtgccgtc cagcggcatt gtcagctacc gcgaagtcac ggcggcgatg
     2641 gcaaaaattt tccaggccag aggcggcgag attatctata acgccgaagt cagcggcctt
     2701 agtgagcata aaagcggcgt ggtgatacgt acccgtcagg gcagcgacta tgaagcatcg
     2761 acgctgatta gctgttccgg gctgatggct gaccggctgg tgaaaatgct cggcctcgaa
     2821 ccgggcttta ttatctgccc gttccgcggc gagtatttcc gcctggcgcc ggagcataac
     2881 cagattgtta accatctgat ttaccccatt cccgacccgg caatgccgtt tttgggcgtg
     2941 catctcaccc gcatgatcga cggcagcgtc accgtcgggc caaacgcggt gctggctttc
     3001 aaacgcgaag gctatcgcaa gcgcgacttc tcatttagcg acacgctgga gattttgggc
     3061 tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggactggg cgagatgaaa
     3121 aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg
     3181 ttaagcgatc tccagccctg gcccgccggt gtgcgggcgc aggcggtatc gccggacggc
     3241 aagctgattg acgattttct gtttgtcacc accccgcgca cgatccacac ctgcaatgcg
     3301 ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag caaggtacaa
     3361 acgctgttgg caagccagag taaccccgga cgcacgctgc gagcggcacg tagtgtggat
     3421 gccttacacg ccgcgtttaa tcaataacct ttgaaaacag gatgtagcga tgaaactgaa
     3481 cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg acgccaacaa
     3541 tggtgaagtc atcgacgtca ccaatccggc gaacggcgac aagctgggta gcgtgccgaa
     3601 aatgggcgct gatgaaactc gcgccgctat cgacgccgcc aaccgcgccc tgcccgtctg
     3661 gcgtgcgctc accgccaaag aacgcgccaa cattctgcgc aactggttca atttgatgat
     3721 ggagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggtaaac cgctggctga
     3781 agcgaaaggc gaaatcagct acgccgcctc ttttattgag tggtttgccg aagaaggcaa
     3841 acgcatttat ggcgacacca ttcccggtca tcaggccgat aagcgcctga ttgtgatcaa
     3901 gcagccgatt ggcgttacag cggccatcac gccgtggaac tttccggcgg caatgattac
     3961 ccgcaaagcc ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca
     4021 gacgccgttc tctgcgctgg cgctggcgga gctggcgatc cgcgcgggca ttccggctgg
     4081 cgtgtttaac gtggtcaccg gttcggcggg cgcggtgggt aatgaactga ccagcaaccc
     4141 gctggtacgc aaactgtcgt ttaccggctc gaccgaaatt ggccgccagt taatggaaca
     4201 gtgcgcgaaa gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt
     4261 ctttgacgat gccgatctcg ataaagccgt ggaaggcgca ctggcctcga aattccgcaa
     4321 cgccgggcaa acctgcgtct gcgccaaccg tttatacgtg caggacggcg tgtatgaccg
     4381 ctttgccgaa aaattgcagc aggcggtgag caaactgcat atcggcgacg ggctggataa
     4441 aggcgtcacc atcgggccgc tgatcgatga aaaagcggta gcaaaagtgg aagagcatat
     4501 tgccgatgcg ctggaaaaag gcgcgcgcgt agtttgcggc ggtaaagcac atgaacgcgg
     4561 cggcaacttc ttccagccca ccattctggt ggacgtgccg gccaacgcca aagtgtcgaa
     4621 agaagagacc ttcggccccc tcgccccgct gttccgtttt aaagatgaag ccgatgtgat
     4681 cgcgcaagcc aatgacaccg agtttggcct tgccgcctat ttctatgccc gtgatttaag
     4741 tcgcgtcttc cgcgtgggcg aggcgctgga gtacggcatc gtcggcatca ataccgggat
     4801 tatttccaat gaagtggccc cgttcggcgg cattaaagcc tcgggtctgg gtcgtgaagg
     4861 ttcgaagtat ggcatcgaag attacttaga aatcaaatat atgtgcatcg gtctttaact
     4921 ggagaacgcg aatgagcagc aataaagagt taatgcagcg ccgcagtcag gcaattcctc
     4981 gtggcgttgg gcaaattcac cccattttcg ctgatcgcgc ggaaaactgc cgggtgtggg
     5041 acgttgaagg ccgtgagtat cttgatttcg cgggcggcat tgcggtgctc aataccgggc
     5101 acctgcatcc gaaagtggtt gccgcggtgg aagcgcagtt gaaaaaactg tcgcacacct
     5161 gcttccaggt gctggcttac gagccgtatc tggagctgtg cgagattatg aatcagaagg
     5221 tgccgggcga ttttgccaag aaaacgctgc tggttacgac cggttccgaa gcggtggaaa
     5281 acgcggtgaa aatcgcccgc gctgccacca aacgtagcgg caccatcgct tttagcggcg
     5341 cgtatcacgg gcgcacgcat tacacgctgg cgctgaccgg caaggtgaat ccgtactctg
     5401 cgggcatggg cctgatgcca gggcacgttt atcgcgcgct ttatccttgc ccactgcacg
     5461 gcatcagtga agatgatgct atcgccagta tccaccgaat ttttaaaaat gatgctgcgc
     5521 cggaagatat cgccgccatc gtgattgagc cggttcaggg cgaaggcggt ttctacgccg
     5581 cgacgcctgc gtttatgcag cgtttacgcg cgctgtgtga cgagcacggg atcatgctga
     5641 ttgccgatga agtgcagagc ggcgcggggc gtaccggcac gctgtttgcg atggagcaaa
     5701 tgggcgtggc accagatctc accacctttg cgaaatcgat cgcaggcggc ttcccactgg
     5761 cgggcgtcac cgggcgcgcc gaagtgatgg atgccgtcgc tccaggcggg ctgggtggca
     5821 cctatgccgg taatccgatt gcctgcgtgg cggcgctgga agtgttgaag gtgttcgagc
     5881 aggaaaatct gctgcagaaa gccaacgatc tggggcagaa gttgaaagat ggattgttgg
     5941 cgatcgccga aaaacaccct gagatcggcg acgtacgcgg gctgggggcg atgatcgcca
     6001 tcgagctgtt tgaagacggc gctcacaaca agccggacgc caaactcacc gccgagatcg
     6061 tggctcgcgc ccgcgataaa ggcctgattc ttctctcctg cggcccgtat tacaacgtgc
     6121 tgcgcatcct tgtaccgctc accattgaag acgctcagat ccgtcagggt ctggagatca
     6181 tcagccagtg ttttgctgag gcaaagcagt agcgccgctc ctatgccgga ggcgacgctg
     6241 cgcgtcttgt ccggcctacg gggatccagg tcggataagg cgtttacgcc gcatccgaca
     6301 atctgtacgt gaacaggaat aaatctatgt tggccgggta aggcggagcc gctctccggc
     6361 aaaaagaatc aataacaatt atacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc
     6421 caagtgacac actttcgaga ggattcagga tggggcaatc atcgcaacca catgagttag
     6481 gcggcgggct gaagtcacgc catgtcacca tgttgtctat tgccggtgtt atcggcgcaa
     6541 gtctgtttgt cggttccagc gtcgccatcg ccgaagcggg cccggcggta ttactggcct
     6601 atctgttcgc cgggctactg gtggttatga ttatgcggat gttggcggaa atggcggttg
     6661 ccacgcccga taccggttcg ttttccacct atgccgataa agctattggt cgttgggcgg
     6721 gttataccat cggctggttg tactggtggt tttgggtact ggttatcccg ctggaagcca
     6781 acatcgccgc catgatcctg cactcatggg ttccaggcat tcccatctgg ttattttccc
     6841 tcgtcattac ccttgcctta actggcagta atttattaag cgttaaaaac tacggcgaat
     6901 ttgagttctg gctggcgctg tgcaaagtca tcgctatcct ggcctttatt ttccttggtg
     6961 cagtcgcaat tagcggtttt tacccgtatg ccgacgtgag cgggatctca agattgtggg
     7021 atagcggcgg ctttatgccc aacggcttcg gtgcggtatt aagcgcgatg ttgatcacca
     7081 tgttctcgtt tatgggcgca gaaattgtca ccattgccgc cgcggaatcc gacacgccgg
     7141 aaaaacatat tgtccgcgct accaactcgg ttatctggcg tatttctatc ttctatttgt
     7201 gctctatttt tgtcgtagtg gcgttaatac cgtggaatat gccggggctg aaagccgttg
     7261 gatcttatcg ctcggttctg gaattgctca atatacccca tgcgaaatta atcatggact
     7321 gcgtgatatt actttccgta accagttgcc tgaactcggc gctgtatacc gcgtcaagga
     7381 tgctctactc cttaagccgt cgcggtgatg cgcccgcggt aatgggcaaa atcaaccgca
     7441 gtaaaacccc gtatgtggcg gtgttactct ccaccggagc ggcattttta acggtcgtgg
     7501 tgaactatta cgcacctgcg aaagtgttta aattcctgat cgacagctcc ggtgctatcg
     7561 ccctgctggt ttatttagtc atcgccgttt cacagttgcg gatgcgtaaa attctgcgag
     7621 cagaaggaag cgaaattcgc ttgcgcatgt ggctttaccc gtggctcacc tggctggtaa
     7681 tcggctttat tacctttgtg ttggtggtga tgttattccg cccggcgcaa cagttagaag
     7741 tgatctctac cggcttatta gcgataggga ttatctgtac cgtgccgatt atggcgcgct
     7801 ggaaaaagct ggtattgtgg caaaaaacac ccgttcataa tacgcgctga taatgatcag
     7861 gagtcacacc atgaccatta cgtctctgga tggctatcgc tggctgaaga acgatattat
     7921 tcgcggtaat tttcaaccgg atgaaaaatt acgaatgagt ttgctgacat cgcgttatgc
     7981 acttggcgtt gggccgttac gggaagctct ttcgcaactg gtggcggaac ggctggtcac
     8041 ggtggtgaat caaaaagggt atcgggtggc gtctatgtca gagcaggagc tgctcgatat
     8101 tttcgacgcc cgcgccaata tggaagcgat gttagtgagt ctggcgattg cccgcggtgg
     8161 cgatgagtgg gaggcagacg ttctcgcaaa agcgcatctg ctgagtaagc ttgaggcctg
     8221 tgacgccagc gagaaaatgc ttgatgagtg ggatctgcgt catcaggcgt ttcatactgc
     8281 aattgtggcg ggctgtggtt ctcactattt gctgcaaatg cgtgaacggt tgtttgatct
     8341 ggcggcgcgt tatcgattta tctggctgcg gcgaacggtg ctttcggtgg aaatgctgga
     8401 ggataaacac gatcagcacc agaccctgac tgcggcggta ctggcgcgag ataccgcgcg
     8461 cgccagtgag ttaatgcgcc agcatttact gacgccaatt cccattatcc agcaggcgat
     8521 ggctggcaat taactactct tccggaatac gcaacacttg ccccggataa attttatccg
     8581 ggctttttag catcggttta ttcgcttcga agattttatt gtacagatta gcgttaccgt
     8641 agacctgttt ggaaatggca ctcagagtgt cgccagactt aacggtataa aactggctgg
     8701 cagtggctgg tgtcgccgtt ttcacctgat catcaacact ggcaataccg gaaatattcc
     8761 ccaccgcaac aaggattttc tccttcgcct cctgactcag gccgtcacca gtgaccgtcg
     8821 ctttgccatc ggcaatttga atgttcactt tatcggcatc aggtataccg gttttgctca
     8881 gatgctcctg caccttcttc gcctgatcat ctttatcgtg ctgacctgta accgcgtccc
     8941 agagtttttc tccggcatct ttcacaaaat tgaacagacc catagctacc tcattcgtta
     9001 acggaaacac tcagaaagtg tagtagagga acgcggcgcg ttaacgccgc ggggatgagc
     9061 acaggtttaa tcgcgggttt gcacccagaa cgcgtgaatc aaaccgggga tatacccaag
     9121 cagtgtcaac agaatattaa taatgaacgc ccaaccgaac cctttaccga gcagcacgcc
     9181 gagcggcggc agaatgatgg tgatgacgat tctccagaaa cccatatgta ctccctataa
     9241 gaaaattact tattgtttaa aaagagattt tatctcttaa gcgtagtaat tttagcggag
     9301 gctgccagtt tcagcttacg cttttactct catttacgct gctgaggctg gtatatgttg
     9361 catttttagt ttagagttta cttaatttag aaaatactta aataactatg accgaactca
     9421 cgcaattaca ggccagtgct gaacaggcag cggccttatt gaaagcaatg agccacccta
     9481 aacggttgct gattctgtgc atgcttagcg gttcccccgg caccagcgcg ggagagctga
     9541 cgcgcattac cggactgagc gcctctgcga catcacagca tctcgctcgt atgcgggacg
     9601 aagggcttat caacagccaa cgggatgccc aacgcattct atattccatt aaaaatgagg
     9661 cggtaaatgc cattatcgcc accctgaaaa atgtctattg tccgtaagga gtcactatgg
     9721 ctttgacaac catttcgccg catgatgcac aagaattaat cgcacgcggc gcaaagttaa
     9781 tcgatattcg tgacgctgat gaatatcttc gtgaacatat tcctgaagca gatctggctc
     9841 cattatccgt gctggaacag tcaggtctcc cggctaaatt acgtcgcgat caaattatat
     9901 tccactgcca ggcaggtaaa cgcaccagta ataacgccga taaattagca gcgattgccg
     9961 cccccgcaga aatcttttta ctcgaagatg ggattgatgg ctggaaaaaa gcaggattac
    10021 cagtagcggt aaataaatct caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg
    10081 gtggtttaat attaatcggc gttatactgg gttataccgt aaatagcggt ttcttcttat
    10141 taagcggctt tgttggtgcc gggttactgt ttgcaggaat tagcggtttt tgtgggatgg
    10201 caaggttgtt agataagatg ccgtggaacc aatgagcttg agaagcgacg ccggacgcgc
    10261 cctagcagcg acatccggcc tcagtaatta gatcaggaaa tcgtcgagag atttaccttc
    10321 tgccagcgcc tgagcgattg gcttcggtgt acggccctga ccggtccagg ttttagtttc
    10381 accgttaaca tcggtgaatt tatatttcgc cggacgcggc tggcgttttt taccagcacg
    10441 tggtgcagca gcagagctat tacccaataa ctcttccggg ttaattccgt cagctttcat
    10501 cagctccagc caggtgctaa ttttttcctg gcgctctgcc agttcacgct gctgctgttc
    10561 ttcttcttca cgtctttctt tagtgacaat cctgaatttt tcgagcattt cttcaagaac
    10621 gtcaatggag aattcgcgag ccatcgcacg gagggtgcga atgttattta aactttgtaa
    10681 cattacggac ataataaaga aaacctttta acgccaaaac aaaaaagta
//
LOCUS       DAZKIF010000143        10469 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZKIF010000143 DAZKIF010000000
VERSION     DAZKIF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.144"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..96
                     /locus_tag="ACHOTW_003952"
     CDS             <1..96
                     /locus_tag="ACHOTW_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845972.1"
                     /translation="TRGGSLIMKAAGISRATYYRIKNELLIVKSE"
     gene            447..614
                     /locus_tag="ACHOTW_003953"
     CDS             447..614
                     /locus_tag="ACHOTW_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9845973.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(694..1941)
                     /locus_tag="ACHOTW_003954"
     CDS             complement(694..1941)
                     /locus_tag="ACHOTW_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFP9845974.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2013..2927)
                     /locus_tag="ACHOTW_003955"
     CDS             complement(2013..2927)
                     /locus_tag="ACHOTW_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFP9845975.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            3143..4576
                     /gene="cscA"
                     /locus_tag="ACHOTW_003956"
     CDS             3143..4576
                     /gene="cscA"
                     /locus_tag="ACHOTW_003956"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9845976.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(4584..5579)
                     /locus_tag="ACHOTW_003957"
     CDS             complement(4584..5579)
                     /locus_tag="ACHOTW_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9845977.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            5822..6040
                     /locus_tag="ACHOTW_003958"
                     /pseudo
     CDS             5822..6040
                     /locus_tag="ACHOTW_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            6058..7386
                     /gene="dsdA"
                     /locus_tag="ACHOTW_003959"
     CDS             6058..7386
                     /gene="dsdA"
                     /locus_tag="ACHOTW_003959"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP9845978.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(7494..9032)
                     /gene="emrY"
                     /locus_tag="ACHOTW_003960"
     CDS             complement(7494..9032)
                     /gene="emrY"
                     /locus_tag="ACHOTW_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP9845979.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVIFLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(9032..10195)
                     /gene="emrK"
                     /locus_tag="ACHOTW_003961"
     CDS             complement(9032..10195)
                     /gene="emrK"
                     /locus_tag="ACHOTW_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP9845980.1"
                     /translation="MEQINSNKKHSNRRKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         2985 a         2516 c         2349 g         2619 t
ORIGIN      
        1 acccggggcg gttcactcat catgaaggca gcaggaatca gccgagcgac ttactaccgg
       61 ataaaaaacg agctattgat cgtaaaatca gaataaaatt tgaaaggtcg tcaggcttgt
      121 ttcatcaggt aaaaaccagc atttctgatc acaacgcttg ggggcaccta ttggggcact
      181 caccaaaatc gaaagagaaa taaactacaa ttcaaattgt tagctaattt ttcgattcct
      241 gcagggacac cagataccct tcaaaccaaa tctaccttca tcccgtaaac gatgggtttg
      301 gcagcacact tgccctatat ctactcattt ttactgcaac aggttgaaat ctcagcactg
      361 tcagaaagcg ctgatgacta aacagcccag ggccgggcga tgtaaccatc acacagaatc
      421 ctgatagcga aatacggcgt gactcgatac ttcactccgc aatgcattcc ttgatgaatt
      481 cgcaggcccg tgatacacgg gacaggtcgc agaatgacga caatgtcctg gaaatcagcg
      541 aaccgtgtat ccggagtaca tttgagcgac tgtaccagaa catgaatgag gtgtttggat
      601 taggcgatta ttagcagggc taagcatttt agtattatta ttttccggtt gaaggatatg
      661 gagatatcga caacaaccgg aaaaagttta cgtctatatt gctgaaggta caggcgtttc
      721 cataactatt tgctcgcgtt ttttactcag gaagaaaatg ccaaatagca acatcaggca
      781 gacaataccc gaaattgcga agaaaactgt ctggtagcct gcgtggtcaa agagtatccc
      841 agtcggcgtt gaaagcagca caatcccaag cgaactggca atttgaaaac caatcagaaa
      901 gatcgtcgac gacaggcgct tatcaaagtt tgccacgctg tatttgaaga cggatatgac
      961 acaaagtgga acctcaatgg catgtaacag cttcactaat gaaataatcc aggggttaac
     1021 gaacagcgcg caggaaagga tacgcaacgc cataatcaca acaccgataa gtaatgcatt
     1081 ttttggccct acccgattca caaagaacgg aataatcgcc atgcacagcg cttcgagtac
     1141 cacctggaat gagttgagat aaccatacag gcgcgttcct acatcgtgtg attcgaataa
     1201 acctgcataa aagacaggaa aaagttgttg atcaaaaatg ttatagaaag accacgtccc
     1261 aacaataaat atgacgaaaa cccagaagtt tcgatccttg aaaactgcga taaaatcctc
     1321 tttttttacc cctcccgcat ccgccgctac gcactggtga tccttatctt taaaacacat
     1381 gttgatcatc ataaatacag cgccaaatag cgagaccaac cagaagttga tatggggact
     1441 gatactaaaa aatatgccgg caaagaacgc gccaatagca tagccaaaag atccccaggc
     1501 gcgcgctgtt ccatattcga aatgaaaatt tcgcgccatt ttttcggtga agctgtcaag
     1561 caaaccgcat cccgccagat accccaggcc aaaaaagagc gcccccagaa ttagacctac
     1621 agaaaaattg ctttgcagta acggttcata aacgtaaatc ataaacggtc cggtcaagac
     1681 caggatgaaa ctcatacacc agatgagcgg tttcttcaga ccgagtttat cctgaacgat
     1741 gccgtagaac atcataaata gaatgctggt aaactggttg accgaataaa gtgtacctaa
     1801 ttccgtccct gtcaacccta gatgtccttt cagccaaata gcgtataacg accaccacag
     1861 cgaccaggaa ataaaaaaga gaaatgagta actggatgca aaacgatagt acgcatttct
     1921 gaatggaata ttcagtgcca taattacctg cctgtcgtta aaaaattcac gtcctattta
     1981 gagataagag cgacttcgcc gtttacttct cactattcca gttcttgtcg acatggcagc
     2041 gctgtcattg cccctttcgc cgttactgca agcgctccgc aacgttgagc gagatcgata
     2101 attcgtcgca tttctctctc atctgtagat aatcccgtag aggacagacc tgtgagtaac
     2161 ccggcaacga acgcatctcc cgcccccgtg ctatcgacac aatccacaga cattccagca
     2221 aaatggtgaa cttgtcctcg ataacagacc accacccctt ctgcaccttt agtcaccaac
     2281 agcatggcga tctcatactc ttttgccagg gcgcatatat cccgatcgtt ctgtgttttt
     2341 ccactgataa gtcgccattc ttcttccgag agcttgacga catccgccag ttgtagcgcc
     2401 tgccgcaaac acaagcggag caaatgctcg tcttgccata gatcttcacg aatattggga
     2461 tcgaagctga caaaacctcc ggcatgccgg atctccgtca tcgcagtaaa tgcgctggta
     2521 cgcgaaggct cggcagacaa cgcaattgaa cagagatgta accattcgcc atgtcgccag
     2581 cagggcaagt ctgtcgtctc taaaaaaaga tcggcactgg ggcggaccat aaacgtaaat
     2641 gaacgttccc cttgatcgtt cagatcgaca agcaccgtgg atgtccggtg ccattcatct
     2701 tgcttcagat acgtgatatc gactccctca gttagcagcg ttctttgcat taatgcacca
     2761 aaaggatcat cccccacccg acctataaac ccacttgttc cgcctaatct ggcgattccc
     2821 accgcaacgt tagctggcgc gccgccagga caaggcagta gccgcccgtc tgattctggc
     2881 aagagatcta cgaccgcatc ccctaaaacc catactttgg ctgacatttt tttcccttaa
     2941 attcatctga cttacgcata gtgataaacc tctttttcgc aaaatcgtca tggatttact
     3001 aaaacatgca tattcgatca caaaatgtca tagttaacgt taacatttgt gatattcatc
     3061 gcatttatga aagtaaggga ctttattttt ataaaagtta acgttaacaa ttcaccaaat
     3121 ttgcttaacc aggatgataa aaatgacgca atctcgattg catgcggcgc aaaacgcact
     3181 agcaaaactt cacgagcgcc gaggtaacac tttctatccc cattttcacc tcgcgcctcc
     3241 tgccgggtgg atgaacgatc caaacggcct gatctggttt aacgatcgtt atcacgcgtt
     3301 ttatcaacat cacccaatga gtgaacactg ggggccaatg cactggggac atgccaccag
     3361 cgacgatatg atccactggc agcatgagcc tattgcgcta gcgccaggag acgagaatga
     3421 caaagatggg tgtttttcag gtagtgctgt cgatgacaat ggtgtcctct cacttatcta
     3481 caccggacac gtctggctcg atggtgcagg taatgacgat gcaattcgcg aagtacaatg
     3541 tctggctacc agtcgggatg gtattcattt cgagaaacag ggtgtgatcc tcactccacc
     3601 agaaggaatc atgcacttcc gcgatcctaa agtgtggcgt gaagccgaca catggtggat
     3661 ggtagtcggg gcgaaagacc caggcaacac tgggcagatc ctgctttatc gcggcagttc
     3721 attacgtgaa tggactttcg atcgcgtact ggcccacgct gatgcgggtg aaagctatat
     3781 gtgggaatgt ccggactttt tcagccttgg cgatcagcat tatctgatgt tttccccgca
     3841 gggaatgaat gccgagggat acagttaccg aaatcgcttt caaagtggcg taatacccgg
     3901 aatgtggtcg ccaggacgac tttttgcaca atccgggcat tttactgaac ttgataacgg
     3961 gcatgacttt tatgcaccac aaagctttgt agcgaaggat ggtcggcgta ttgttatcgg
     4021 ctggatggat atgtgggaat cgccaatgcc ctcaaaacgt gaaggctggg caggctgcat
     4081 gacgctggcg cgcgagctat cagagagcaa tggcaaactt ctacaacgcc cggtacacga
     4141 agctgagtcg ttacgccagc agcatcaatc tatctctccc cgcacaatca gcaataaata
     4201 tgttttgcag gaaaacgcgc aagcagttga gattcagttg cagtgggcgc tgaagaacag
     4261 tgatgccgaa cattacggat tacagctcgg cactggaatg cggctgtata ttgataacca
     4321 atctgagcga cttgttttgt ggcggtatta cccacacgag aatttagacg gctaccgtag
     4381 tattcccctc ccgcagcgtg acacgctcgc cctaaggata tttatcgata catcatccgt
     4441 ggaagtattt attaacgacg gggaagcggt gatgagtagt cgaatctatc cgcagccaga
     4501 agaacgggaa ctgtcgcttt atgcctccca cggagtggct gtggtgcaac atggagcact
     4561 ctggcaactg ggttaacata atatcaggtg gaacaacgga tcaacagcgg gcaagggatc
     4621 cgcgtcacac ttcccccttc acgaccttca ataatatgca atgcagcttc ccgcccgata
     4681 atgtcatgtg gaagctgaat tgtggtcagc ggcggtaaaa acagatgccc gacgccaacc
     4741 agattatcaa agcccattac ggcgacatcc tgcgggatac gtaccccctt agccaggaga
     4801 acctgataag ccacaaaggc tgcgcgatcg ttaccacata tcagaacatc aaaatctggt
     4861 ttgcccgatt tgaagtggtc attgagtaaa cttgcgagat cggtgtagtg atcatcacct
     4921 gttgccatgt gaaattgttt cacctcagcc agatctcgtc cagcatcacg ccaggcctgc
     4981 tcaaatccct gccgacgata ccctgttgcc aacgcacttt ccggtagcca gaagcataac
     5041 ggttgacgat agcccgccgc gagcaaatgc tgtgttgatt catattgtgc agtgtaatca
     5101 tcagggatat aactgggtaa cgctgggtca tccgccacac agttcgccaa tacaatattt
     5161 tcaccataca gagactcagg tagcgtgata tgtcgcagcc ccattgtagt atagataatg
     5221 ccatccggac ggtgggcaag cagctgacgt gccgcgcggg cagcgtcatc ttcagaaaaa
     5281 atattgatta aaaaactatt ccagccgaac tcgctggcgg tttgctcaat ggcaagcaga
     5341 atatcaacag agaaaggagt ggtagcagtg tcctgcgcca gcacggcgag agtcgacggc
     5401 ttacgtcctt gagcgcgcat cttacgggcg gaaagatcag gaacataatt cagggtctgg
     5461 attgcctgca atacgcggtc acgcgttgca ggacgcacag attctgcatt atgcatcacc
     5521 cgggagactg tcatcatcga cactcccgcc aggcgtgcga catcctttaa tgaagccata
     5581 cccaagccgt ttgccgtaaa acgggcactg tagcagaaac agacgtcact ggcgagatcc
     5641 aacgaaattc tcgacgccct atcacccgac acggcaatac aataaaaaat aacaataatt
     5701 gccggataat tgtacccaat tccgcctctg ttctcgccaa cgagtgacca tataggcaag
     5761 taagaaaaac atgccaactg gcatcgccaa cctggagaag aaaatataaa tatggatcat
     5821 tgtgctgccg ctgtatcccg acatcagccc ggaaattatt gcgattgcta tcggttcagg
     5881 cgcgattggc tgcacgatcg ttacggactc gcttttctgg ctggtgaagc aatattgcgg
     5941 cgctacgctc aatgaaacat ttaaatacta tacgacagcg acatttatcg cttcagtcgt
     6001 cgctctggcg ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag acgtactatg
     6061 gaaaacgcta aaatgaactc gctcatcgcc cagtatccgt tggtaaagga tctggttgct
     6121 cttaaagaaa ccacctggtt taatcctggc acgacctcat tggctgaagg tttaccttat
     6181 gttggcctga ccgaacagga tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc
     6241 tatctggcaa aagcatttcc tgaaactgct gccactgggg ggattattga atcagaactg
     6301 gttgccattc cggctatgca aaaacggctg gaaaaggaat atcaacaacc gatcagcggg
     6361 caactgttac tgaaaaaaga tagccatttg cccatttccg gctccataaa agcacgcggc
     6421 gggatttatg aagtcctggc acatgcagaa aaactggctc tggaagcggg gttgctgacg
     6481 cttgaagatg actacagcaa actgctttct ccggagttta aacagttctt tagccagtac
     6541 agcattgctg tgggctcaac cggaaatctg gggttatcaa tcggcattat gagcgcccgc
     6601 attggcttta aggtgacagt gcatatgtct gctgatgccc gggcatggaa aaaagcgaaa
     6661 ctgcgcagcc atggcgttac ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag
     6721 gaaggacgta aagcagcgca gtctgacccg aactgtttct ttattgatga cgagaattcc
     6781 cgcacgttgt tccttgggta ttccgtcgca ggccagcgtc ttaaagcgca atttgccgag
     6841 caaggtcgta ttgtcgatgc tgataaccct ctgtttgtct atctgccgtg tggtgttggc
     6901 ggtggtcctg gtggcgtcgc attcggactt aagctggcgt ttggcgatca tgttcactgc
     6961 ttttttgccg aaccaacgca ctccccttgt atgttgttag gcgtccatac aggattacac
     7021 gatcagattt ctgttcagga tattggtatc gacaacctta ccgcagcgga tggccttgca
     7081 gttggtcgcg catcaggctt tgtcgggcgg gcaatggagc gtctgctgga tggcttctat
     7141 acccttagcg atcaaaccat gtatgacatg cttggctggc tggcgcagga agaaggtatt
     7201 cgtcttgaac cttcggcact ggcgggtatg gccggacctc agcgcgtgtg tgcatcagta
     7261 agttaccaac agatgcacgg tttcagcgca gaacaactgc gtaatgccac tcatctggtg
     7321 tgggcgacgg gaggtggaat ggtgccggaa gaagagatgg agcaatatct ggcaaaaggc
     7381 cgttaataac gtttcaacgc agcatcgcaa tcctttccct gggtgagcga tgctgccgat
     7441 ggcgcagact taagatcccc agtcttaccc gctataaccc cctgctttta ccatcaccca
     7501 acgcctttcg ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag taagataaaa
     7561 gcaatagccg ccatacggaa aatctcattt gcagaaatag aaagtgactg ctgggttatt
     7621 tcattattaa tttcattaag aactcctgaa agcgaaccat agtatttatc cataatttgt
     7681 gacgatgaat taaacacggg gttaaattga tcgatggttt ctgtcaactg actatggtgt
     7741 aacgattcgc gtcgtcccca cagcgtcatt gtcaacgacg taccaactga tcctgacaag
     7801 gtacgaaaaa aattactcat actcgaggca ttggcaaatt tattatctgg caagcctgaa
     7861 aacgaaatcg ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg aaaaaactgc
     7921 ggcaaaatga tacctgtaaa atcaatcgtt ggcataaatg tcacagaacg ccagtaatag
     7981 caaaccgcat acatcaaaaa actgaatgtc accaacaccc gcatgtctat tttgttgcca
     8041 taacgtccta tcaaaggtga aattaatagt ggcatgatgc cgatgggcgc ataagcaagt
     8101 ccggcccata tcgcattata ccccatcgtt tcctggagta actgcggcat aaggacgatc
     8161 gctccagagt aaaataaata cgcgcatgtg atactcacaa taccaatggt gaagttacgg
     8221 gacttaaaca aactgagatc aagaatcggg ttctctgagg tcgactccca aatgactaaa
     8281 gagatcagaa aaataactga tactactgtt aatattatta ttgtactcga gttgaaccaa
     8341 tccagatcgc gccctttatc aagcataatt tgcaagccac caacaccgag cactaacagg
     8401 gtcagtcctg gtagattcat tttgaccggt gaagtctcag tttctcttcc tttaagtaag
     8461 gttaagcata atgtcaggac gataatcccc atagggacat tgattaaaaa tatccaaccc
     8521 cagctaaagt tatcacaaat ataaccgccc aatatcggcc cacatatcgg agcgataatc
     8581 acggtcattg accataatgc cagagcaaat gttctttttt ctggtggata attccttaat
     8641 aataaactct gtgacagtgg aattaacggc cctgccatta acccctgaac gactctaaaa
     8701 aatatcagca catcaagatt gatcgataaa ctacacatta aggaagacag cgaaaaaaaa
     8761 gtgactgaaa gtaaaaataa ccttaattcg cctattcttt gtgccagcct gccggtaaca
     8821 gggatcgcaa tggcatttgc tacgccaaac gaggtgataa cccaggtgcc ttcgtctgtt
     8881 gatgctccca gaaagccaga tattgtcggt attgcgacgt tagaaatagt ggaatccaac
     8941 atttgcataa acgtcgctaa tgacaatgca atagtgacgc accataacgt cccaccggtt
     9001 aatggtgccg gagttgattt agtgattgcc attaaagttg tccattatgc gaaataatgt
     9061 tgctaatttc tttttctatc ggactggtat cgataactaa agccttactg gtataagccg
     9121 gcatggacgt cacggttgaa gccagctcag gcatctcagc aatgtcttcg ttcttcgtat
     9181 caatagttgc agtcatcgat aaaccaatgc gcaaggggtg ttccatgagt tcttttggat
     9241 caagagaaac ttcaaccggt acacgctgaa cgattttgat ccagttccct gtcgcatttt
     9301 gtgcaggtaa taaggagaac gcattgccgg ttcccatatt gatccctgtc acccggccat
     9361 gaaacacaac attctcacca taaagatcgc tgataatatt gaccgattga ccaatccgta
     9421 catccgtgag ttgtgtttct ttaaagttag cattaaccca catttgacgt gccggtacga
     9481 cagccattaa cgattgtccg ggactcactg tttcgccgac ctgaacactt ctctgggcaa
     9541 tatagccggt aaccggactc ttaatatccg tacgtttaag cgccaaccag gcttctttgg
     9601 ttgcatccgc agcttcaacg acttgtggct gacggtttaa tggtgtgttc attactaaag
     9661 ctttattcgc tttataagcc tggatagcgg cattcaatgc cgctttgcta cttattaacg
     9721 tatctttggt atgctccagc gtttcttttg aaataacccc ctgcttcgct aaaggcacac
     9781 gacggttata atcttctaaa gattgttgat actgaatacg tgctgaagcg acttcggcac
     9841 tgtattgttt atcctgtaag tacagtttat tcgtttgccg aacaatattt gccagattat
     9901 ttttagcttt attgagtgcg atagtggcat cagttttatc cagtgaaact aaaatgtcac
     9961 cctgtcgaac gtagttcgta tctttatgat taacgacagt gacactacct gagacttgtg
    10021 cagaaattgg atctgcattc cccgtgacat aggcgtcatc tgtactaatc atgtcttcta
    10081 attccattga ccaataggca taagcacctg aaaacgcaat aaataaaact atcaccaata
    10141 aagaaaagta ttttcttctg ttagaatgtt ttttatttga attaatctgt tccactatta
    10201 tctctcattt cgcatagatg aaatttatga attgaatgaa aagagtattc attcttcata
    10261 tcaaacacag tgcatgtgtt gcacattatc cctggaacgt tggaggccta atttaaatga
    10321 ggtgcaaaaa aaacaacaga ggactaaacc gtggcttttg caatacaatt cttacgcctg
    10381 taggattagt gagaagactt atagtgctaa cttgaaacta taaatcatcg gtataatccc
    10441 tgattttatt gttgacattt catatatgc
//
LOCUS       DAZKIF010000144        10280 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZKIF010000144 DAZKIF010000000
VERSION     DAZKIF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.145"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            127..1809
                     /gene="ydeN"
                     /locus_tag="ACHOTW_003962"
     CDS             127..1809
                     /gene="ydeN"
                     /locus_tag="ACHOTW_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9845981.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1861..3018
                     /gene="ydeM"
                     /locus_tag="ACHOTW_003963"
     CDS             1861..3018
                     /gene="ydeM"
                     /locus_tag="ACHOTW_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9845982.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2984..3106)
                     /locus_tag="ACHOTW_003964"
     CDS             complement(2984..3106)
                     /locus_tag="ACHOTW_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9845983.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3309..4994
                     /gene="yddA"
                     /locus_tag="ACHOTW_003965"
     CDS             3309..4994
                     /gene="yddA"
                     /locus_tag="ACHOTW_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9845984.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            5032..7404
                     /gene="yddB"
                     /locus_tag="ACHOTW_003966"
     CDS             5032..7404
                     /gene="yddB"
                     /locus_tag="ACHOTW_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP9845985.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7461..10244
                     /gene="pqqL"
                     /locus_tag="ACHOTW_003967"
     CDS             7461..10244
                     /gene="pqqL"
                     /locus_tag="ACHOTW_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9845986.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3006 a         2283 c         2259 g         2732 t
ORIGIN      
        1 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
       61 ttacatccac ttatttctct tcgtaaaatt actttggaat taagtacaat aagaagagga
      121 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
      181 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
      241 aaaacaaacg ttgctttctc agactttacg ccgactgaat acagtaccaa aggaaagcca
      301 aatattatcg tactgaccat ggatgatctt ggttatggac aacttccttt tgataaggga
      361 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
      421 gataaagcca ttgaagctgc gcaaaaatca acgccgacgc tcctttcatt aatggatgaa
      481 ggcgtacgtt ttactaacgg ctatgtggcg cacggtgttt ccggcccctc ccgcgccgca
      541 ataatgaccg gtcgagcgcc cgcccgcttt ggtgtctatt ccaatactga tgctcaggat
      601 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
      661 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
      721 caaacacgtg actatcatga caacttcacc acattttctg cggaagaatg gcaaccgcaa
      781 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
      841 ccttcactgt tcaagaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
      901 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
      961 tacctggctt ataatgctcc gcacctgcca aatgataatc ctgcaccgga gcaatatcag
     1021 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     1081 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     1141 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     1201 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     1261 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     1321 tacccgacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggc
     1381 gtttccttgc tgccctggtt gcaagataag aaacaaggcg agccacataa aaatctgacc
     1441 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataactac
     1501 cacaaatttg tccgtcatca gtcagacgat tacccgcata accccaacac tgaggactta
     1561 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     1621 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     1681 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     1741 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     1801 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     1861 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     1921 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     1981 gagttcatca aacaatatat cgcagcgtct ggcaatcagg tctattttac ctggcaaggc
     2041 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     2101 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     2161 gaatggtgtg cctttctcaa agaacatgag tttctggttg gtatctcgat cgatggaccc
     2221 caggagctac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     2281 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     2341 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     2401 aaacatatgc aatttatcga attgctcgaa accgggacgc cgaatattga tttcagtggt
     2461 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     2521 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcatccgt
     2581 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc ataccagttg tattttccag
     2641 gagtcctgca aggataatct ggttgttgaa agtaatggag atatttacga atgcgatcat
     2701 tttgtctatc cacagtacaa acttggaaac attaataaat ctgaacttaa aacgatgaac
     2761 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     2821 aaacctatct gcaatggcgg ttgccctaag catcgtatta ctaaagtaaa cgatgagact
     2881 gtttcctatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     2941 gccatggtgg agttagccaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     3001 caaatggaga ataattaata acgctctctt ttagctccat tcttagcctc cttcttatga
     3061 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     3121 gcgaaaatct tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     3181 agcataacca taaccactga aaattaaaaa tttctatata tttcatattg ttaaatgata
     3241 cttaaataaa tttcatcagt aaatcattgc catagataat cattggatgg cacaatcaat
     3301 tttcgctaat gataaccatt cccattacgt tttgtatgtt gatagcaaag tatctctgtc
     3361 tgctgaagcc cttttggctg cgtaagaata ataaaacttc agtgttgtta atcattatta
     3421 tcctcgccat gatccttggc gtggttaaaa ttcaggtctg gttaaacgac tggaataatg
     3481 attttttcaa tgcgctaagc cagaaagaaa ccgataagct ttggcagctt gtcctttggt
     3541 tccctgccct gctggggatc tttgtattaa tatccgtaaa caaaacatgg ttaattaaac
     3601 tactgactat tcgctggcgc gaatggttaa cagattacta ccttaatcgc tggtttgcag
     3661 ataaaaacta ctacttcacg cagatctacg gcgagcataa aaatacagat aaccctgacc
     3721 aacgtatcgc tgaagacatt ctcttactga tcagcaaaac gctcagtctt tcatttggct
     3781 tcatccagtc acttagcatg ctgatcacct ttaccgttat tctttggcaa agtgcgggta
     3841 tgctctcatt cactgttggt ggaacagaat ggaatatcca ggggtatatg gtctataccg
     3901 ttgtgctcat tgtgatcggt ggaactttat ttacccataa ggtgggtaaa cgtattcgtc
     3961 cgcttaatgt ggaaaaacaa cgtagcgaag cgacatttcg aactaatctt gtgcagcata
     4021 ataagcaggc agaactgatt gcgctaagca atgctgagtc tctacaacgc caggagttga
     4081 gcgacaattt tcataccatt aaagagaact ggcatcgttt aatgaatcgt cagcggtggc
     4141 ttgattactg gcagaatatt tattcgcgct cgcttagcgt tcttccctac tttctgttat
     4201 tgccgcagtt tattagcggg cagatcaatc tgggcggact gatgaaatcg cgccaggcat
     4261 ttatgctggt atcgaacaat ctaagctggt ttatttataa atatgatgaa cttgctgaac
     4321 tggctgcggt tatcgatcgc ttgtatgagt tccatcaact caccgaacag cgccctacga
     4381 ataagcctaa aaattgccaa catgcggtac aagtggctga cgcgagtatt cgtactcctg
     4441 ataataagat catattagag aacctgaact ttcatgtttc gccaggcaaa tggctattac
     4501 tgaaaggtta ctctggtgcg ggaaaaacca cactgcttaa aacattatcc cactgctggc
     4561 cgtggtttaa aggtgatatt tcttctcctg ccgacagttg gtatgtgtca caaacaccgt
     4621 taatcaaaac cggcttactg aaagagatta tttgtaaagc acttcccctg cccgtagacg
     4681 ataaatcatt gagcgaagta ctgcatcagg ttggtcttgg gaaattggct gcgcgtattc
     4741 atgaccacga tcgctgggga gatattcttt ccagcggcga aaaacaacgt atcgccctgg
     4801 cacgattaat tttacgacgt ccgaaatgga tatttcttga cgaaactacc tctcatcttg
     4861 aggaacaaga ggctatccgc ttactgcgtc tagtgcgtga aaaactaccc acaagcggcg
     4921 tcattatggt tacacatcaa cccggtgtct ggaacctggc cgatgatatt tgtgacatta
     4981 gcgcggtttt ataaaaacaa taaaagccct gctgtacgga gatataaata aatgaagcga
     5041 gttcttattc ctggcgtcat tttatgtggc gctgatgtgg cgcaggccgt cgatgacaaa
     5101 aacatgtaca tgcatttttt tgaagagatg acggtctatg ctcctgtccc tgtacccgta
     5161 aacggcaaca cgcattacac cagtgaaagc atcgagcgtt taccgaccgg gaatggcaat
     5221 atcagcgatc tgctgagaac caaccctgcg gtacgcatgg attcaacgca aagtacctcg
     5281 ttgaaccagg gagatattcg cccggagaaa atctctattc acggtgcgtc gccctatcag
     5341 aatgcctatt tgattgacgg tattagtgcc actaataacc tgaacccagc gaatgagtcc
     5401 gatgccagta gtgcaaccaa tattagcggg atgtcacagg ggtattatct tgatgtcagc
     5461 ttactggaca atgtgacgct ttatgacagt tttgtgccgg ttgaattcgg tcgcttcaat
     5521 ggcggggtaa ttgatgcaaa gttcaaacgc ttcaacgctg atgatagcag cgtgaaactg
     5581 ggttatcgca ctacgcgttc ggactggtta acatcgcata tcaatgagaa taacaagagc
     5641 gcatttaatc aaggctcttc aggaagtact tattactccc cagattttaa aaagaacttt
     5701 tataccttgt cgtttaatca ggaactcgct gataactttg gcgttaccgc cggtttatcg
     5761 cgccgccagt ctgatatcac ccgcgcggat tatgtttcga atgacggcat tgtcgccggt
     5821 cgggcacagt ataaaaacgt tatcgatact gcattgagca aatttacctg gtttgccagc
     5881 gaccgcttta cccacgattt aaccttaaaa tataccggct ccagccgtga ttataatacc
     5941 agcaccttcc cgcagtctga tcgcgaaatg ggtaataaat cctatggtat ggcatgggat
     6001 atggatacgc agctcgcatg ggccaaacta cgtaccaccg ttggttggga tcatattagt
     6061 gattataccc gtcacgatca tgacatctgg tacaccgaac tttcatgtac atatggtgat
     6121 attacagggc gttgcacccg tggcggatta ggacacattt cccaggctgt agataattac
     6181 accttcaaaa cacgcctgga ctggcaaaaa ttcgccgtgg gtaatgtttc gcatcaaccc
     6241 tacttcggcg cggaatacat ctattccgat gcgtggactg aacgccataa ccagtctgaa
     6301 tcctatgtga ttaatgctgc cggaaagaaa actaaccata ccatttacca taaaggtaaa
     6361 ggcagcctgg gaattgacaa ctacacactg tatatggcgg atcgcattag ctggcgtaat
     6421 gtgtcattaa tgcccggcgt gcggtatgac tatgacaact atctgtcaaa ccacaatatc
     6481 tccccgcgct ttatgacgga atgggatatt tttgctgatc aaacctcaat gattacagca
     6541 ggttataacc gttactatgg cgggaatatt cttgatatgg gattacgtga tatccgcaat
     6601 agctggacgg aatcggtatc aggtaataaa accctgacgc gttatcagga tttgaaaacg
     6661 ccttataacg atgaactggc aatgggattg cagcaaaaaa tcggtaagaa cgttattgcg
     6721 cgcgcaaact atgtttaccg tgaagcgcat gatcaaatca gcaaaagcag tcgtaccgac
     6781 agcgcgacta aaaccaccat tactgaatat aacaacgacg gcaaaaccaa aacgcattcg
     6841 ttcaacctca gttttgaact ggccgaaccc ctgcatatca gccaggtaga tattaacccg
     6901 caaattgtct ttagctatat caagagcaag ggcaacttgt cgttaaacaa tggttatgag
     6961 gagagcaata ccggtgataa ccaggtggtt tataacggta atcttgtctc ttacgatagc
     7021 gttccagtgg cagattttaa taacccatta aagatctcct taaacatgga tttcacgcat
     7081 caaccgagcg ggttggtgtg ggcgaatacg ctggcctggc aagaagcgcg taaagctcgc
     7141 attatcctgg gtaagacaaa tgcgcaatac atcagcgaat attcagatta caagcagtat
     7201 gttgacgaaa aactggatag cagcctgacc tgggacaccc gcttgtcctg gacgccacaa
     7261 tttctgaaac aacaaaacct gacgatcagt gccgatattc tcaatgtact ggatagcaaa
     7321 accgcggttg atacaacgaa taccggtgtg gcgacctacg ccagtggccg tactttctgg
     7381 cttgatgtca gcatgaaatt ttaatagttc ggattttact taaccgggta aattcgcccg
     7441 gttttcgcat ggagataatt atgagaaacc tctgtttctt actgacgtta gtggcaactc
     7501 tgttgctccc cgggcggctg attgccgccg ccttaccgca ggatgaaaag ttaattaccg
     7561 ggcaactgga caatggcttg cgatatatga tttatccgca tgctcatcca aaggatcagg
     7621 taaatttatg gctgcaaatt cataccggtt cattgcagga agaagacaat gagcgcggcg
     7681 tggctcattt tgtagaacat atgatgttta acggcacaaa aacatggccg ggtaataaag
     7741 tcatcgaaac atttgagtca atgggcctgc gttttggtcg cgatgttaat gcctatacca
     7801 gctatgacga aacggtgtat caggtgagtt tgccaactac gcagaaacaa aatctgcaac
     7861 aagtgatggc aatcttcagt gaatggagta atgccgcaac ctttgaaaaa ctcgaagtag
     7921 acgctgaacg cggcgtaatt actgaggaat ggcgtgccca tcaggatgcg aaatggcgca
     7981 cctctcaggc gcgccgccct ttcctgctgg caaatacccg taatttagac cgtgaaccta
     8041 tcggcctgat ggatactgtc gccacggtca caccggcaca attgctccaa ttttatcaac
     8101 gctggtatca accaaataat atgaccttta tcgtggttgg cgatatcgac agtaaagaag
     8161 cgctggcgct gataaaggat aatttaagta agcttccggc taacaaagca gctgaaaatc
     8221 gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa tatcattaat gataaagaaa
     8281 accgggtgaa cggcatcgca ctctattatc gcctgccaat ggtacaagtg aacgatgagc
     8341 aaagctttat cgaacaagct gaatggagca tgttagttca gctgttcaat caacgtctgc
     8401 aggaacgtat acagtcgggc gagttgaaga ctatttctgg cggcactgcg cgcagcgtta
     8461 aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa tgcacgagac gataatatgc
     8521 aggatgctgc gaatgcatta atggaagagt tggcaaccat tgatcagcat ggtttttctg
     8581 ctgaagaact cgatgatgtc aaatctaccc gcctcacctg gctgaaaaat gcggttgatc
     8641 agcaagctga acgtgattta cgtatgctga ccagtcgcct ggcatccagc tcattaaata
     8701 atacgccgtt cttgtcgccg gaagagacat atcaactttc gaaacgtctg tggcagcaaa
     8761 ttaccgtgca aagtctggcg gaaaaatggc agcagttaag aaagaaccag gacgcatttt
     8821 gggagcaaat ggtaaacaat gaggttgccg ccaaaaaagc attgtctcct gcggctatcc
     8881 tggcgctgga aaaggagtac gccaacaaaa agctggcggc ttacatcttc ccaggcagaa
     8941 atttatcgtt aacagtagac gctgacccac aggcggaaat tagcagcaaa gaaacgctgg
     9001 ctgagaatct gacatcatta acactttcca atggtgcaag ggttattctg gcaaaatccg
     9061 cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa taaaggcgat ttaagtttcc
     9121 ctgcgcagca aaaatcactt atcgcgctgg caaataaagc agttagcgga agcggcgttg
     9181 gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga aaactcggta accatgagca
     9241 gtaaagtcag tggcatgaat acgttgctct ccgttagcgc gcggactaat aaccctgaac
     9301 ctggttttca gttgattaac cagcgaatca cccacagcac gattaacgac aatatttggg
     9361 catcgctaca aaatgctcaa attcaggcat tgaaaacgct cgaccagcgt ccagcggaga
     9421 aattcgccca gcagatgtat gagacgcgct atgctgatga ccgcacgaaa ttactgcaag
     9481 aaaatcagat tgcacagttt actgccgcag atgcgctggc tgccgatcgc cagttgtttt
     9541 catctccagc ggatatcacg tttgtcattg tcggtaatgt cgcagaagac aaactcgtgg
     9601 cgttaattac gcgttactta ggatcaatca aacactctga ttcgccatta gccgcaggta
     9661 aaccattaac tcgcgcgacg gacaacgcat cggttactgt aaaggaacaa aatgaacctg
     9721 tggcacaggt ttcacagtgg aagcgttatg attcccggac acctgttaat ctggcgacgc
     9781 gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga tctacgcgtt aatattcgtg
     9841 aacaggcatc tggggcatac agcgtttctt ctcgcctctc ggttgatcct caggctaaag
     9901 atatcagtca tttgctggct tttacttgtc aaccagaacg acatgatgaa ctgttaacgt
     9961 tagcgaatga agtgatggtt aagcgtttga ctaaagggat cagtgagcaa gaactgaacg
    10021 aataccagca aaacgttcag cgcagcctcg atatccaaca gcgtagcgtt caacaattag
    10081 cgaacactat tgtaaatagt cttattcaat atgacgatcc tgcagcatgg actgagcagg
    10141 agcaattgtt gaaacaaatg acggtagaga atgttaacac tgccgttaaa caatatcttt
    10201 ctcatccggt aaatacttat accggagtat tattgccaaa ataataacag ccccgtcaac
    10261 acatcgttgg cggggatttt
//
LOCUS       DAZKIF010000145        10241 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZKIF010000145 DAZKIF010000000
VERSION     DAZKIF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.146"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(113..2686)
                     /gene="clpB"
                     /locus_tag="ACHOTW_003968"
     CDS             complement(113..2686)
                     /gene="clpB"
                     /locus_tag="ACHOTW_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9845987.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="ACHOTW_003969"
     CDS             complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="ACHOTW_003969"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP9845988.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="ACHOTW_003970"
     CDS             complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="ACHOTW_003970"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP9845989.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4659..5396
                     /gene="bamD"
                     /locus_tag="ACHOTW_003971"
     CDS             4659..5396
                     /gene="bamD"
                     /locus_tag="ACHOTW_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP9845990.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5667..6008
                     /gene="raiA"
                     /locus_tag="ACHOTW_003972"
     CDS             5667..6008
                     /gene="raiA"
                     /locus_tag="ACHOTW_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9845991.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6111..6236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6112..6159
                     /gene="pheL"
                     /locus_tag="ACHOTW_003973"
     CDS             6112..6159
                     /gene="pheL"
                     /locus_tag="ACHOTW_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP9845992.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6258..7418
                     /gene="pheA"
                     /locus_tag="ACHOTW_003974"
     CDS             6258..7418
                     /gene="pheA"
                     /locus_tag="ACHOTW_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP9845993.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="ACHOTW_003975"
     CDS             complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="ACHOTW_003975"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP9845994.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="ACHOTW_003976"
     CDS             complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="ACHOTW_003976"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP9845995.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9873..10238
                     /gene="yfiL"
                     /locus_tag="ACHOTW_003977"
     CDS             9873..10238
                     /gene="yfiL"
                     /locus_tag="ACHOTW_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP9845996.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
BASE COUNT         2628 a         2705 c         2397 g         2511 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat cattactgga
      121 cggcgacaat ccggtcttca ttaacttcca ggcgaataac tttacccgga accaattcac
      181 cagacagtat ttgctgtgcc aacgggtttt cgatctgctg ctggatagca cgtttcaaag
      241 gacgtgcacc atagactgga tcgtaaccgt tctcgctcag cagtttcagc gcctcgtcag
      301 aaatgtggat ttcataacca cgttcttcca gacgtttgta cagacgtttc aactgaatct
      361 gcgcaatcga ggcaatgtgc tgttcgccca gcggatggaa gaccaccact tcatcgatac
      421 ggttaatgaa ctccggacgg aagttatggc ttaccacacc gagcaccagc tctttcatgt
      481 gcgcataatc cagttcaccg aagcgttcct gaatcagatc ggaaccgagg ttagaggtca
      541 taatgacgac cgtattacgg aagtcgaccg ttctcccttg cccgtcagtc agacgcccat
      601 catccagtac ctgcaacaga atgttgaaga catccggatg cgctttttcc acttcatcca
      661 gcaggatgac ggaatacgga cgacgacgca ccgcttcggt caggtagcca ccttcttcat
      721 aaccgacata tcccggaggc gcaccaacca gacgagacac cgagtgtttc tccataaact
      781 cggacatatc gatacggacc atcgcctcgt cgctatcaaa cataaagttc gccagcgcct
      841 tacaaagctc tgttttcccc acaccagttg ggccgaggaa caggaatgaa ccaatcgggc
      901 gatttggatc cgccagcccc gcacggctac gacgaatagc gttagatacc gcatcaaccg
      961 cttcgttctg accaattacg cgatggtgca gttcttgctc catacgcagc agtttttcgc
     1021 gctcgctttc catcatgcga gaaaccggaa tccccgtcca acgcgccagc acttcagcaa
     1081 tttcggcgtc ggtcacttta ttacgcaaca gacgcatagt tttgccttcg agctgcgttg
     1141 cggcttccag ttgcttttcc agttccggga ttttgccgta ttgcagttca gacatccgcg
     1201 ccaagtcacc cacacggcga gcctgttcaa tagcgatttt cgcctgttcc agttccgctt
     1261 taatggtctg cgtaccagaa agcgatgcct tctctgcttt ccactcttct tctaactcgg
     1321 agtactgacg ttctttgtcg ctcagttctt cgttgagcat atccagacgt tttttactgg
     1381 cttcatcaga ctctttcatt aacgcctgtt gttccagttt gagctggatg atacggcgat
     1441 cgagtcggtc gagttcttct ggttttgagt caatctgcat acgaatgctg gatgctgctt
     1501 catcgatcag gtcgatggct ttatccggca gctgacggtc agcaatgtag cgatgagaca
     1561 acgtcgccgc tgcaacaatt gccgggtcag taatttgcac atggtggtgc aattcgtaac
     1621 gttctttcag gccacgcaga atcgcaatgg tatcttcaac agaaggctcg gcaacaaaca
     1681 ctttctggaa acgacgttcc agcgcagcat ctttttcgat gtactggcga tattcgtcaa
     1741 gcgtcgtggc acctacgcag tgcaattcac cacgcgccag cgccggtttc agcatgtttc
     1801 cggcgtccat tgcgccatcg gctttacccg cgccgaccat ggtatgtaat tcgtcgataa
     1861 acaggatgac gttgccttcc tgtttggcaa gatcgttaag cacgcctttt aaacgttctt
     1921 caaactcacc gcgatatttc gccccagcca ccagcgcgcc catatccagc gccagtaccc
     1981 ggcggccttt caacccttcc ggcacttcgc cgttgataat acgctgcgcc agaccttcaa
     2041 cgatggcagt tttaccgacg ccgggttcac caatcagtac cgggttattt ttagtacgac
     2101 gttgcagcac ctgaatggta cggcgaattt cttcatcacg accaatcacc ggatcgagtt
     2161 tgccctgttc ggctcgttcg gtaaggtcga tggtatattt tttcaaagcc tgacgttggt
     2221 cttcagcacc ttgatcgttc acgctttcac ctccacgcat ttgttcaatc gcttgagtaa
     2281 tgttggcggt ggtcgcccct gctgctttca ggatgtcggc cagcgtgccg cgagactcaa
     2341 gtgccgccag aacgaacagt tctgacgaga taaagttatc accacgtttt tgcgccagct
     2401 tgtcgcaaag attaagaacg cgcaccagat cctgtgatgg ctggacatca ccaccagtac
     2461 cttcaacctg cggtaaacga tttaatgcct gattgatatc tgtgcgcaac tggccagcat
     2521 ttatgccagc ggatgttaat aaaggactaa ccgaaccccc ttcctgattc agcagggcgc
     2581 tcattaaatg aagtggttcg ataaattggt tgtcgtgccc aagtgcaagt gattgggcat
     2641 cggcaagagc aagctggaat ttattagtaa gacgatccag acgcataact cctcccataa
     2701 cggatcaaag ttgctactgg agattaaatg aggtcatccc tcaattattc aaggttattg
     2761 accagattaa tgtgaaaaga aaatcacgcg taccggatcg tcttgattct ttaggttata
     2821 tcagccaaat gaaacttgcc atacgaccgg tggtcttgtc gcgacgataa gagaagaaag
     2881 tctcattttc cgtatatgta caacggtcgc cgccgaaaat ttgctcaaca cccacgttcg
     2941 ccagacgctg ccgggcaagc tgataaatgt ccgccagata cttatcacca tgctgaatga
     3001 aagctgtact cgcttcggcg tctactgcca taaacgcctc gcgaacctcc gcccccactt
     3061 caaacgcgcg tgggccaatt gccggcccta accaggcgag aatattttct ggattatcag
     3121 caaaacagga aactgtctct tccagcacgc ctgcgcacag tccacgccag ccagcatgag
     3181 cggcggcgac ttccgttccc gctcgattgc aaaacagcac agggaggcag tcggcagtca
     3241 tcactgcgca aaccctgccg ggcgtattgc tataagaggc atctgctcgt tttgaggcat
     3301 aaggttcgcc agtaagatta agcacatctt tgccgtgtac ctgctcaagc cagaccggtt
     3361 tagaaggcaa attgcccgca gcaaaaagtc gcttgcgatt ctcctcaacg tgatccgggt
     3421 tatcgccaca atgggcaccg aggttgagcg agtcatacgg gggcaagctc acgccgccga
     3481 tacgagtaga gctacaggcc gcaacacctt ttggcagcgg ccactgcggg acaatcagct
     3541 tactcataac cagtccactt catctttatg ctcttcgaaa tcggcgcgca tcacctcaat
     3601 cagctccacc atatcttgtg gaataggcgc atgccattcc atttcgatac cggagatcgg
     3661 gtgataaaga cgcagcatgg ttgcatgaag cgcctggcgg tcaaatttac gcagcgtgga
     3721 gataaacgct tccgaagcac cttttggcgg gcgcggacgg ccaccataaa ccggatcgcc
     3781 caccagcgga tgagtgatat gggccatatg cacgcggatc tggtgcgtac gtccagtttc
     3841 cagacgcaac cgcagacgcg tgtgcacacg gaagtgttcc atgatgcgat agtgagtcac
     3901 cgccggtttg cccatcggat gcaccgccat gtgagtacgt ttggtcgggt ggcggctgat
     3961 tggttcatct actgtaccgc cagcggtcat atgaccaatt gccaccgctt catactcacg
     4021 agtaatttca cgcctttgca aagattcgac taaacgcgtc tgagccggaa cggtttttgc
     4081 cacaaccatc aggccagtgg tgtctttatc cagacgatgg acgatgcctg cacgcggtac
     4141 atcagcaatg ggcggatagt aatgaagtaa cgcattcagt accgtgccat ccgggttacc
     4201 cgcgccagga tgtaccacca gatcgcgcgg tttattaatg acgatgatat cttcatcttc
     4261 atagacgata tccagcggga tatcctgcgg ttcaaaacgc gcttcttctt caatctcagc
     4321 gttgatggca acctgctcgc cacccaatac tttttctttc ggcttatcac aaagtttgcc
     4381 gttaaccagc acgcgctggt cgaggatcca ttcttttatt cgcgaacgtg aataatccgg
     4441 gaacatttcg gccaaagcct gatctaagcg ttgaccgagt tggttttcgg acaccgttgc
     4501 agtgagctgt actcgttgtg ccatatatac tgcttcttcg tttatcgttg gggttttacg
     4561 gctttgccgt ttaatatagt gtgctattgt agctggtctt aaccgggagc aggaacagag
     4621 aatctcccgt attacatttt gaggaaagtc aaaacgtcat gacgcgcatg aaatatctgg
     4681 tggcagccgc cacactaagc ctgtttttgg cgggttgctc ggggtcaaag gaagaagtac
     4741 ctgataatcc gccaaatgaa atttacgcga ctgcacaaca aaagctgcag gacggtaact
     4801 ggagacaggc aataacgcaa ctggaagcgt tagataatcg ctatccgttt ggtccgtatt
     4861 cgcagcaggt gcagctggat ctcatctacg cctactataa aaacgccgat ttgccgttag
     4921 cgcaggctgc catcgatcgt tttattcgcc ttaacccgac ccatccgaat atcgattatg
     4981 tcatgtacat gcgtggcctg accaatatgg cgctggatga cagtgcgctg caagggttct
     5041 ttggcgttga ccgtagcgat cgcgatcctc aacatgcacg agctgcgttt agtgactttt
     5101 ccaaactggt gcgcggctat ccaaacagtc agtacaccac cgatgccacc aaacgtctgg
     5161 tattcctgaa agatcgtctg gcgaaatatg aatactccgt ggccgagtac tatacagaac
     5221 gtggcgcatg ggttgccgtc gttaaccgcg tagaaggcat gttgcgcgac tacccggata
     5281 cccaggctac gcgtgatgcg ctgccgctga tggaaaatgc ataccgtcag atgcagatga
     5341 atgcgcaagc tgaaaaagta gcgaaaatca tcgccgcaaa cagcagcaat acataacaga
     5401 aacctgaaac acaaaacggc agcccttgag ctgccgtttt tttattctgt cagttgtgaa
     5461 actgaagcga tttagtcact atcgttctca tcaaatatgg ctcgctttga gatattcctc
     5521 aagtaaaaaa acacctcttc ctgcgatttc tcacaaaaaa gattcgttga caaaaagtga
     5581 caaaattatg agatttccat cacacatttt gacatcagga acggtatgct gaattcacca
     5641 agacgggaag acaagaggta aaatttatga caatgaacat taccagcaaa caaatggaaa
     5701 ttactccggc catccgccaa catgtcgcag accgtctcgc caaactggaa aaatggcaaa
     5761 cacatctgat taatccacat atcattctgt ccaaagagcc acaagggttt gttgctgacg
     5821 ccacaatcaa tacacctaac ggcgttctgg ttgccagtgg taaacatgaa gatatgtaca
     5881 ccgcaattaa cgaattgatc aacaagctgg aacggcagct caataaactg cagcacaaag
     5941 gcgaagcacg tcgtgccgca acatcggtga aagacgccaa cttcgtcgaa gaagttgaag
     6001 aagagtagtc ctttatattg agtgtatcgc caacgcgcct tcgggcgcgt tttttgttga
     6061 cagcgtgaaa acagtacggg tactgtacta aagtcactta aggaaacaaa catgaaacac
     6121 acaccgtttt tcttcgcatt cttttttacc ttcccctgaa tgggaggcgt ttcgtcgtgt
     6181 gaaacagaat gcgaagaaga acaataaggc ctcccaaatc ggggggcctt ttttattgat
     6241 aacaaaaagg caacactatg acatcggaaa acccgttact ggcgctgcga gagaaaatca
     6301 gcgcgctgga tgaaaaatta ttagcattac tggcagagcg gcgcgaactg gccgtcgagg
     6361 tgggaaaagc caaactgctc tcgcatcgcc cggtacgtga tattgatcgt gaacgcgatt
     6421 tactggaaag attaattacg ctcggtaaag cgcaccatct ggacgcccat tacattactc
     6481 gcctgttcca gctcatcatt gaagattccg tattaactca gcaggctttg ctccaacaac
     6541 atctcaataa aattaatccg cactcagcac gcatcgcttt tctcggcccc aaaggttctt
     6601 attcccatct tgcggcgcgc cagtatgctg cccgtcactt tgagcaattc attgaaagtg
     6661 gctgcgccaa atttgccgat atttttaatc aggtggaaac cggccaggcc gactatgccg
     6721 tcgtaccgat tgaaaatacc agctccggtg ccataaatga cgtttacgat ctgctgcaac
     6781 ataccagctt gtcgattgtt ggcgagatga cgttaactat cgaccattgt ttgttagtct
     6841 ccggcacgac tgatttatcc accatcaata cggtctacag ccatccgcag ccattccagc
     6901 aatgcagcaa attccttaat cgttatccgc actggaagat tgaatatacc gaaagtacgt
     6961 ctgcggcaat ggaaaaggtt gcacaggcaa aatcaccgca tgttgctgcg ttaggaagcg
     7021 aagctggcgg cactttgtac ggtttgcagg tactggagcg gattgaagcg aatcagcgac
     7081 aaaacttcac ccgatttgtg gtgttggcgc gtaaagccat taacgtgtct gatcaggttc
     7141 cggcgaaaac gacgttgtta atggcgaccg ggcaacaagc cggtgcgctg gttgaagcgt
     7201 tgctggtact gcgcaaccac aatctgatta tgacccgtct ggaatcacgc ccgattcacg
     7261 gtaatccatg ggaagagatg ttttatctgg atattcaggc caatcttgaa tcagcggaaa
     7321 tgcaaaaagc attgaaagag ttaggggaaa tcacccgttc aatgaaggta ttgggctgtt
     7381 accctagtga gaacgtagtg cctgttgatc caacctgatg aaaaggtgcc ggatgatgtg
     7441 aatcatccgg cactggatta ttactggcga ttgtcattcg cctgacgcaa taacacgcgg
     7501 ctttcactct gaaaacgctg tgcgtaatcg ccgaaccagt gctccacctt gcggaaactg
     7561 tcaataaacg cctgcttatc gccctgctcc agcaactcaa tcgcctcgcc gaaacgctta
     7621 tagtaacgtt tgattaacgc cagattacgc tctgacgaca taataatgtc ggcataaagc
     7681 tgcggatcct gagcgaacag tcgcccgacc atcgccagct caaggcggta aatcggcgaa
     7741 gagagcgcca gaagttgctc aagctgaaca ttttcttctg ccaggtgcag cccgtaagca
     7801 aaagtagcaa agtggcgcag tgcctgaata aacgccatat tctgatcgtg ctcgacggcg
     7861 ctaatacgat gcaaccgagc gccccagacc tgaatttgct ccagaaacca ttggtatgct
     7921 tccggtttac gtccatcaca ccagaccaca acttgctttg ccaggctacc gctgtccgga
     7981 ccgaacattg ggtgtaaccc cagtaccggg ccatcgtgcg ccgccagcat ggcctgtaat
     8041 ggtccatttt tcactgatgc cagatcaacc agaatacaat ctttcggtaa aggcggtaat
     8101 ttgccaataa cttgctcagt aacgtggatt ggcacactaa caatcaccat tccggcatcg
     8161 gcaacaatat cagccgctcg atcccagtca tgttgctcca gaatccgcac ctgataaccc
     8221 gagagtgtca gcatcttctc gaacagacgt cccatctgac cgccgccgcc aacgataact
     8281 accgggcgta acgcaggaca aagcgtttta aatcctttgt cgttttcact ggagtaagat
     8341 tcacgcatca cccgacgcaa aacatcctca atcagatctg gcggtacacc cagagcttcc
     8401 gcctctgcac gacgcgaggc caacatagat gcctctcgct ccggaacata aataggcagt
     8461 ccaaagcggc ttttcacctc gcccacttca gcaaccagtt ccagacgctt cgctaataaa
     8521 ttcagcagcg ctttatcgac ttcatcaatt tgatcgcgta atgcggtcaa ttcagcaacc
     8581 ataataaacc tcttaagcca cgcgagccgt cagctgcccg ttcagatcct gatgaatttc
     8641 acgcagcaag gcatcggtca tttcccagct aatgcaggca tcggttacgg atacaccgta
     8701 tttcatttca ctgcgcggtt gctcggaaga ctgattgccc tcgtggatat tactttcgat
     8761 catcagacca ataattgagc gattgccatc tttgatttga gcaaccacgg attctgccac
     8821 cgcaggctga cggcgataat ctttattgga attaccgtgg ctgcaatcta ccatcagaga
     8881 cgggcgcagt cccgcctgtt ccatctcttt ttcacattgc gcaacatccg cagggctata
     8941 gttcggcgct ttaccaccgc gcaggatgac atgcccgtcc ggattcccct gagtttgtag
     9001 caacgcaacc tgccctgcct ggttaatgcc aacaaaacgg tgcggctggg cggcggcgcg
     9061 catagcgtta attgctgttg ccagactgcc gtcggtgccg tttttaaagc caaccggcat
     9121 ggaaagcccg gaggccattt cacggtgagt ttgcgattcc gttgtacgag caccaattgc
     9181 tgaccagcta aacagatcgc ccaggtattg cgggctattc ggatctaacg cttccgtcgc
     9241 cagtggcagt cccatattca ccagctcaag cagcaattta cgcgcgatct gcagcccggc
     9301 ttctacatca aaagagccat ccatatgggg atcgttaatt aaccctttcc agccgacagt
     9361 ggtacggggt ttttcaaaat agacgcgcat taccagatag aggctatcgc tgacctctgc
     9421 ggcaagggct ttaaatcgac gagcatattc cagagcagtt tccggatcat gaatggaaca
     9481 agggccacat actaccagca gacgaggatc gcgcccggcg ataatatctg aaatggtttt
     9541 acgcgagtca gcaatctggg cttcttgttg caggcttaat ggaaaagcgg ccttcagttg
     9601 tgccggagtc attaaaacct gttcgtcggt aatatgtacg ttattcagcg cgtctttttg
     9661 catgatggcg atcctgttta tgctcgtttg cgatagttga tcctcagcga ggatgacgta
     9721 acgataacac ataaagtaaa gttttcaatc catatttcgt acatttttct ttacataagc
     9781 aatttattcg cgctttcaac ccttgattct gtatagataa atttacacca cctttgaaaa
     9841 caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaagtttatc gcccccttgt
     9901 tggctttact ggttagcgga tgccagattg atccttatac tcacgcgcca accttgacca
     9961 gcaccgactg gtatgatgtt ggtatggaag atgcgatatc gggcagcgcc ataaaagatg
    10021 acgatgcatt tagcgattca caggcggatc gcggtctata ccttaaagga tatgccgaag
    10081 gacaaaagaa aacttgccag accgatttta cttatgcccg aggactttcc ggtaaaagct
    10141 ttcctgccag ctgcaacaat gttgaaaatg ccagccaact gcatgaagta tggcaaaaag
    10201 gggctgatga aaatgccagc gcaatacgat taaattaatt a
//
LOCUS       DAZKIF010000146        10137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZKIF010000146 DAZKIF010000000
VERSION     DAZKIF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.147"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            63..638
                     /gene="ssuE"
                     /locus_tag="ACHOTW_003978"
     CDS             63..638
                     /gene="ssuE"
                     /locus_tag="ACHOTW_003978"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9845997.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            631..1590
                     /gene="ssuA"
                     /locus_tag="ACHOTW_003979"
     CDS             631..1590
                     /gene="ssuA"
                     /locus_tag="ACHOTW_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP9845998.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1587..2732
                     /gene="ssuD"
                     /locus_tag="ACHOTW_003980"
     CDS             1587..2732
                     /gene="ssuD"
                     /locus_tag="ACHOTW_003980"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP9845999.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            2744..3535
                     /gene="ssuC"
                     /locus_tag="ACHOTW_003981"
     CDS             2744..3535
                     /gene="ssuC"
                     /locus_tag="ACHOTW_003981"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP9846000.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIVFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3532..4299
                     /gene="ssuB"
                     /locus_tag="ACHOTW_003982"
     CDS             3532..4299
                     /gene="ssuB"
                     /locus_tag="ACHOTW_003982"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9846001.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4342..6954)
                     /gene="pepN"
                     /locus_tag="ACHOTW_003983"
     CDS             complement(4342..6954)
                     /gene="pepN"
                     /locus_tag="ACHOTW_003983"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP9846002.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7220..8422
                     /gene="pncB"
                     /locus_tag="ACHOTW_003984"
     CDS             7220..8422
                     /gene="pncB"
                     /locus_tag="ACHOTW_003984"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9846003.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8591..9991
                     /gene="asnS"
                     /locus_tag="ACHOTW_003985"
     CDS             8591..9991
                     /gene="asnS"
                     /locus_tag="ACHOTW_003985"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9846004.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2264 a         2610 c         2761 g         2502 t
ORIGIN      
        1 cagactgagc gaattgttat caatgttaac aaaacaagaa caattggtta taaggagaga
       61 gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc ttctcgctcc agctccttgc
      121 tggaatatgc gcgggaaaaa ctaaatggcc tggatgttga ggtttatcac tggaatctgc
      181 aaaacttcgc cgcggaagat ctgctttatg ctcgtttcga tagtccggca ctccatacct
      241 tcaccgaaca gttgcaacag gctgacgggc tgattgtcgc cacgcctgtg tataaagccg
      301 cttattccgg tgcgctgaaa accctgctcg acctgctgcc agaacgtgcg ttgcaaggca
      361 aagtggtgct accgctggcg acgggcggta cggtggccca tctgctggcg gtcgattatg
      421 cccttaagcc agttttaagt gcgctgaaag cccaggagat cctgcacggc gtgtttgccg
      481 atgactcaca agtaattgat taccatcaca aaccccagtt cacgccgaat ttacaaaccc
      541 gtcttgatac cgcgctagaa actttctggc aggcattgca ccgccgcgat gttcaggttc
      601 ctgatcttca ttctctgcga ggtaatgccc atgcgtaaca tcattaaact ggcgctggtg
      661 ggattgctta gcgtctctac gattgtggtt gctgcagaat cctcgcctga agcgttacgt
      721 ataggctatc agaaaggcag tattggtatg gtattggcaa aaagccacca gttactggaa
      781 aaacgctatc cgcaatcaaa aatctcgtgg gtggagttcc ccgctggccc gcaaatgctt
      841 gaggcgttaa acgttggcag tattgatctc ggcagtaccg gggatattcc gccaatcttc
      901 gcccaggctg ccggggctga tttgctgtac gtgggcgtcg agccgccgaa gccaaaagcc
      961 gaggtgattc tggtggcaga aaacagcccg atcaaaaccg cagccgatct taaaggccac
     1021 aaagttgcgt tccagaaagg ttccagttca cacaatcttt tactgcgcgc actacgccag
     1081 gccggactta agtttactga catccagccc acttatctga cgccagctga tgcccgcgcc
     1141 gcgttccagc aaggtaacgt tgacgcctgg gctatctggg atccctacta ctccgctgca
     1201 ttattacagg gcggcgtgcg ggtgttgaaa gacggcaccg atctcaatca aaccggatcg
     1261 ttttatctgg cagctcgccc ctatgcagaa aaaaacggcg cttttattca gggtgtactg
     1321 gcaaccttta gtgaggccga tgcgttaacc cgcagccagc gcgagcaaag tatcgcttta
     1381 ctggcaaaaa cgatgggctt accggcaccg gtgattgcct cttacttaga tcatcgccct
     1441 cctaccacca tcaaaccggt taacgccgag gttgccgcct tacagcagca aacggcagat
     1501 ctgttttatg aaaatcgtct ggtgccgaaa aaagtcgata ttcgccaacg catctggcag
     1561 cccactcaac tggaaggaaa acaattatga gtctgaatat gttctggttt ttaccgaccc
     1621 atggtgacgg gcattatctg ggaacggaag aaggttcacg cccggttgat cacggttatc
     1681 tgcaacaaat tgcgcaagcg gcggatcgtc ttggctatac cggtgtgctg atcccaacgg
     1741 ggcgctcctg cgaagatgcg tggctggttg ccgcatcgat gatcccggtg acgcagcggc
     1801 tgaagtttct tgtcgccctg cgtcccagcg taacctcacc taccgttgcc gcccgccagg
     1861 ccgccacgct tgaccgtctc tcaaatggac gtgcgttgtt taacctggtc acaggcagcg
     1921 atccacaaga actggcaggc gacggcgtgt tccttgatca tagtgagcgc tacgaagcct
     1981 cggcggaatt tacccaggtc tggcggcgtt tattgctggg ggaaaccgtc gatttcaacg
     2041 gtaaacatat tcatgtgcgg ggagccaaac tgctcttccc gccgattcaa cagccgtatc
     2101 ctccgcttta ctttggcgga tcgtcagacg tcgcccagga gctggcggca gaacaggttg
     2161 atctctacct cacctggggc gaaccgccgg aactggttaa agagaaaatc gaacaagtgc
     2221 gggcgaaagc tgccgcgcat ggacgcaaaa ttcgtttcgg cattcgtctg catgtgattg
     2281 ttcgtgaaac taacgacgaa gcgtggcagg ccgccgagcg gttaatctcg catcttgatg
     2341 atgagactat cgccaaagca caagccgcat tcgcccgcac ggattccgtg gggcaacagc
     2401 gaatggcagc gctacataac ggcaagcgcg acaatctgga gatcagcccc aatttatggg
     2461 cgggcgttgg cttagtgcgc ggcggtgccg ggacggcgct ggtgggcgat ggtcctacgg
     2521 tcgctgcgcg aatcaacgaa tatgccgcgc ttggcatcga cagctttgtg ctttcgggct
     2581 atccgcatct ggaagaagcg tatcgggtcg gcgagttgct gttcccacat ctggatgtcg
     2641 ccattccgga aattccccag ccgcagccgc tgaatccgca aggcgaagcg gtggagaatg
     2701 attttatccc tcgcagagtc gcgcaaagct aaggaagaag ataatggcaa cgccagtgaa
     2761 gaagtggtta ttgcgcgttg ccccctggtt tttaccggtg ggcatcgtga cggtgtggca
     2821 actggcctcc tcggttggct ggttgtcgac gcgtattttg ccctcaccgg aaggggtggt
     2881 gatggcgttc tggacgctct cagccagcgg cgaattgtgg cagcatctgg cgatcagctc
     2941 ctggcgggcg ctgattgttt tttcaattgg cggctcgctg gggttgattt tgggactgat
     3001 tagcggactg tcgcgctggg gagaaaggct gctggatacc tcaattcaga tgttgcgcaa
     3061 cgtgccgcat ctggcgctga tcccgctggt gattttgtgg tttggcattg atgaatccgc
     3121 aaaaatcttt ctggtggcgc tcggtacgct attccccatt tatatcaaca cctggcatgg
     3181 catccgtaat atcgatcgcg ggttggtgga gatggcgcgt agctatggat tatccggcat
     3241 accgctgttt atccatgtga tcctgcctgg tgccctgccc tcaattatgg tcggcgtgcg
     3301 ttttgcgttg ggcctgatgt ggctgacgct gattgttgcc gaaaccattt ctgccaattc
     3361 aggcattggt tatctggcga tgaatgcgcg ggagtttttg caaacggacg tggtggtagt
     3421 cgctattatt ctttacgccc tgctcggcaa acttgctgac gtcagcgcgc agctgttaga
     3481 gcgcctctgg ttgcgctgga acccggctta tcatttgaag gaggccacgg tatgaatact
     3541 gctcgtctga accagggcac accattgttg ctcaatgcag taagcaaaca ttacgcggaa
     3601 aatatcgtcc tgaaccaact ggatttacat attccggcag gtcagtttgt ggcggtggtg
     3661 ggccgcagcg gtggtggcaa aagtaccctg ctgcgcctgc tggcaggtct ggaaacgcca
     3721 accgctggcg atgtgttagc gggcaccaca ccgctggctg aaattcagga agacacgcga
     3781 atgatgtttc aggatgcgcg tctgctaccg tggaaatcgg tgattgataa cgttgggtta
     3841 ggccttaaag gccagtggcg cgatgccgcg cgtcgggcgt tggctgcggt aggactggag
     3901 aatcgcgccg gggaatggcc tgccgcactt tctggcgggc agaaacagcg agtggcgctg
     3961 gcaagagcgt tgattcatcg accgggttta ttgttgcttg atgaaccgct cggtgcgctg
     4021 gatgccttaa cgcgactcga gatgcaggat ttgattgtgt cactttggca ggagcacggt
     4081 tttaccgtac tgttggtgac gcatgatgtg agcgaagcgg tagcaatggc tgatcgggtg
     4141 ttgttaattg aagagggaaa aattggtctg gatttgacgg tggatattcc ccgtccgcgc
     4201 cggttagggt cggtgcgatt agcggagctg gaagcggaag tgttgcaacg ggtgatgcag
     4261 cgtggtgaat ctgaaactcg cctgagaaaa caggggtaaa atccccgaat ggcggcgcta
     4321 ttgccgccat tcggggattt attaagccag tgctttggtg atcttctcgt acagatcgcc
     4381 agatagattt tccagccctt tcaactgttc cagcgccgcg cgcattttct cctgacgttt
     4441 ggcatcataa cgtttcaggc gaatcagcgg ttcaatcagg cgtgaagcta cctgcgggtt
     4501 acggctgttc aggtcggtaa gcatttccac caggaactgg taaccgctcc catcttcggc
     4561 atggaacgct gccgggttgc tgcccgcaaa cgcaccaatc aacgaacgaa tgcggttcgg
     4621 gttgctcatg gtaaatgagc gatgctgcaa caggccgcgc accgtctcca gcacattcgc
     4681 cgccgggctg gtggcttgca ggataaacca tttatccatc accagaccat cctgatgcca
     4741 cttgtcgtcg tactcctgca tcagcgcgtc acggcaaggc agctgtgcag caaccgccgc
     4801 agaaagcgct gccagcgcat cggtcatatt gtttgcttca tggtattgct tgctcaccag
     4861 cacgtcagcc agatgcgttt caccaaaagc aaggaagcgc aggcaggcat tacgcagagt
     4921 gcgttttgca atatcttcat gctcaacacg gtactcgctc tggtaattcg cgttgtaaat
     4981 agccagtagc tcatccgcca gttcagtcgc cagagtacga gtgagtgctt cgcgtacttc
     5041 ggcaatagca atcgggtcga tgatatcgaa cagttcagcc atttcattga cagaaggcag
     5101 cgtcaggatt tctgccgcca gcgccggatc aatcttctca tcgagcagca ccgcgcggaa
     5161 agcgtcagcc acatgtaccg gcagagacag cggctgccct tgctgatgac gggcgacgtt
     5221 cagcttgatg taggttgcca gcaaactttg cgccgcatcc caacgggaaa aatcattacg
     5281 cgcatgacgc atcaggaagg tcagttgctg atcgctccac ttatattcca gtttcactgg
     5341 cgcagagaat tcgcacagca gcgcaggcac cggctggaag tagacattat caaaaacaaa
     5401 ggtctgttcc gcctgggtga cgttcagcac ggaattcacc ggatgaccgc ctttctgcaa
     5461 cgggatcact ttgccttcgt tgtcatacaa ttcgatggca aacggaatat gcagcggctg
     5521 tttttctgct tgatccggcg tggctggcgt gcgctggctg atggtcaggg tgtactgctc
     5581 ggtttccgga ttgtagtcgt ctttgacggt cacaaccggc gtaccggact ggctgtacca
     5641 acggcggaaa tgggagagat cgacattcga cgcatcttcc atcgcctgaa cgaaatcgtc
     5701 gcaggtcgct gcactgccat catggcgctc gaaatagagc tgcatccctt tctggaagtt
     5761 ttcttcgcca agcagagtgt ggatcatacg aatcacttcc gcgcccttct cgtaaacggt
     5821 cagggtgtag aaattgttca tctcaatgac catgtccggg cggattgggt gcgccatcgg
     5881 gctggcgtct tccgcaaact gcaatccgcg catggtgcgc acattattga tacggtttac
     5941 cgcgcgggaa ccaaggtcag agctgaattc ctgatcgcgg aagacggtta aaccttcttt
     6001 caggctgagc tggaaccagt cgcggcaggt cactcggtta ccggtccagt tatggaaata
     6061 ttcatggccg ataacgcgtt caatatcgag gtaatctttg tcggtggcgg tatcggtgcg
     6121 ggccagcaca tatttggagt taaagatatt cagaccctta ttctccattg cgcccatatt
     6181 gaagaaatcc accgcgacga tcatatagat gtcgaggtca tactccaggc caaagcgttc
     6241 ttcatcccat ttcatggagt ttttcagcga ggtcatcgcc cacggcgcgc gatcaaggtt
     6301 gccgcgatcg acgtacagct ccagtgctac ttcgcgacca gaacgcgtgg taaaggtatc
     6361 gcgcagtaca tcaaagtcgc ctgccaccag cgcaaacagg tagcacggtt tcgggaacgg
     6421 gtcctgccac tgtacccaat ggcgtccgtt ttccagttcg ccttgcgcaa cgcggttacc
     6481 gttggaaagc aggaagggat atttggtttt atcggcaata attttggtgg taaaacgcgc
     6541 cagcacgtcc gggcggtcga gataatacgt aatatggcgg aaaccttcgg cttcacactg
     6601 ggtgcaaagc gcatcgcctg actgataaag cccttccagc gccgtattcg ccgccgggct
     6661 tatttcatta atgatcttaa gcgtaaaacg ctccggcaaa ttactgatga ccagtgcgcc
     6721 ctcttcttct ttccaggcgg tccacggctc atcattaata tgaacagaaa ccagtttgag
     6781 gtcttcgccg ttgagacaaa gcggagcatc tgatgcacca tgacggacag cctggctgac
     6841 cgcggtaacg accgtctttt gcgcgtcgag gtcaaaggtc aagtcaatat cagtaatctg
     6901 gtaatccggc gcacgataat cgtgacggta tttggcttgt ggctgttgag tcataaataa
     6961 cctttagcat cttttataga gtctggtgtt cagtctattc ctgttgcgta aatcgcgcta
     7021 tgcagaatct tcatcttttc aggtacaaac gcctttattg ctacattttt ataacataca
     7081 ccgcgtaatg ccatcgacca gaaaggtggc atatggtgtg atcggggttc aataaattgc
     7141 gaaacaaggt atactccagc agttcctgaa gatgtttatt gtactaaacg ctcctgtacg
     7201 aggacgctac tgcgcaccta tgacacaatt cgcttctcct gttctgcact cgttgctgga
     7261 tacagatgct tataagttgc atatgcagca agccgtgttt catcattatt acgatgtgca
     7321 tgtcgcggcg gagtttcgtt gccgaggtga cgatctgctg ggtatttatg ccgatgctat
     7381 tcgtgaacag gttcaggcga tgcagcacct gcgcctgcag gatgatgaat atcagtggct
     7441 ttctgccctg cctttcttta aggccgacta tcttaactgg ttacgcgagt tccgctttaa
     7501 cccggaacaa gtcaccgtgt ccaacgataa tggcaagctg gatattcgtt taagcggccc
     7561 gtggcgtgaa gtcatcctct gggaggttcc tttgctggcg gttatcagtg aaatggtaca
     7621 tcgctatcgc tcaccgcagg ccgacgttgc gcaagccctc gacacgctgg aaagcaaatt
     7681 agtcgacttc tcggcgttaa ccgctggtct tgatatgtcg cgcttccatc tgatggattt
     7741 tggcacccgt cgccgttttt ctcgcgaagt acaagaaacc atcgttaagc gtctgcaaca
     7801 ggaatcctgg ttcgtgggca ccagcaacta cgatctggcg cgtcggcttt ccctcacgcc
     7861 gatgggaaca caggcacacg aatggttcca ggcacatcag caaatcagcc cggatctagc
     7921 caacagccag cgagctgcac ttgctgcctg gctggaagag tatcccgacc aacttggcat
     7981 tgcattaacc gactgcatca ctatggatgc tttcctgcgt gatttcggtg tcgagttcgc
     8041 tagtcggtat cagggcctgc gtcatgactc tggcgacccg gttgaatggg gtgaaaaagc
     8101 cattgcacat tatgaaaagc tggaaattga tccacagagt aaaacgctgg ttttctctga
     8161 caatctggat ttacgcaaag cggttgagct ataccgccac ttctcttccc gcgtgcaatt
     8221 aagttttggt attgggactc gcctgacctg cgatatcccc caggtaaaac ccctgaatat
     8281 tgtgattaag ttggtagagt gtaacggtaa accggtggcg aaactttctg acagccctgg
     8341 caaaactatc tgccatgata aagcgtttgt tcgggcgctg cgcaaagcgt tcgaccttcc
     8401 gcatattaaa aaagccagtt aatatcatca gggagctaat cggctccctt tttttacctt
     8461 taattccgaa atctttcgct gcatttgcga attctgcttg tctgattgca gatgccaggt
     8521 aacataggta tccccccatt tacgggtgga caagtgttta ttttttccga ctattaacag
     8581 agagaatatt atgagcgttg tgcctgtagc cgacgtactc cagggccgcg tagccgttga
     8641 cagcgaagtc accgtacgcg gatgggtacg tacccgccga gattcaaaag ctggcatctc
     8701 cttcctcgcc gtttatgacg gttcctgctt tgatcctgta caggctgtca tcaataattc
     8761 tctgcccaat tacaatgaag acgtcctgcg tctgaccacc ggctgctcgg tcattgtgac
     8821 gggtaaagtc gtggcgtcgc cgggccaggg gcaacaattt gaaattcaga ccagcaaggt
     8881 tgaagttgct ggttgggttg aagatccaga cacttacccg atggcggcaa aacgccacag
     8941 cattgagtat ctgcgtgaag tcgctcacct gcgtccgcgc acaaacctga ttggtgccgt
     9001 cgcgcgcgtt cgccatacgc tggcgcaggc gctgcatcgc ttctttaacg agcagggatt
     9061 cttctgggtt tcaacgccac tgattactgc gtctgatacc gaaggggctg gcgaaatgtt
     9121 ccgcgtttct acgctggatc tggaaaatct gccgcgtaac gatcagggca aagtggattt
     9181 cgacaaagac ttctttggta aagagtcttt cctgaccgta tctggccagt tgaacggcga
     9241 aacctacgct tgcgcattat ccaaaattta taccttcggc ccgactttcc gtgctgaaaa
     9301 ctccaacacc agccgtcacc tggcggaatt ctggatgctg gagccggaag tggcgtttgc
     9361 taacctgaac gatatcgcgg gtctggctga agccatgctg aaatatgtct tcaaagcggt
     9421 tctcgaagaa cgcgctgacg acatgaaatt cttcgctgaa cgcgtagata aagatgccgt
     9481 ttcacgtctg gaacgcttta ttgaggccga tttcgcgcag gtggattaca ccgaagcagt
     9541 aaccattctc gaaaactgcg gcaggaagtt tgaaaacccg gtttactggg gcgtcgatct
     9601 ctcttctgag catgagcgtt atctggcgga agaacacttt aaagcaccgg tagtggttaa
     9661 aaactatccg aaagatatta aagcgttcta tatgcgcctc aacgaagacg gtaaaaccgt
     9721 tgcggctatg gatgttctgg ctccgggcat cggtgagatc attggtggct cccagcgtga
     9781 agagcgtctg gacgtgctgg acgagcgtat gctggaaatg ggcctgaaca aagaagatta
     9841 ctggtggtat cgcgatctgc gtcgctacgg tactgttccg cattccggtt tcggtcttgg
     9901 ttttgaacgc ctgattgctt acgtaactgg tgtgcaaaac gtgcgtgatg tgattccgtt
     9961 cccacgaact ccgcgtaacg ccagcttcta atttagcgtc tacaagagcc agcacccgct
    10021 ggctcttttt tatcctttct gtcaagttat ctgtttgtta agtcaatcaa tctatttgca
    10081 accccgccat aacgatcatc ctgttacgga atattacatt gcaacattta cgcacaa
//
LOCUS       DAZKIF010000147        10107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZKIF010000147 DAZKIF010000000
VERSION     DAZKIF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.148"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            6..81
                     /locus_tag="ACHOTW_003986"
     tRNA            6..81
                     /locus_tag="ACHOTW_003986"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHOTW_003987"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHOTW_003987"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHOTW_003988"
     CDS             complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHOTW_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9846005.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHOTW_003989"
     CDS             complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHOTW_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9846006.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWVVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHOTW_003990"
     CDS             complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHOTW_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9846007.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3595..4620)
                     /locus_tag="ACHOTW_003991"
     CDS             complement(3595..4620)
                     /locus_tag="ACHOTW_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9846008.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5051..5272)
                     /locus_tag="ACHOTW_003992"
     CDS             complement(5051..5272)
                     /locus_tag="ACHOTW_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846009.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5525..8674)
                     /gene="acrF"
                     /locus_tag="ACHOTW_003993"
                     /pseudo
     CDS             complement(5525..8674)
                     /gene="acrF"
                     /locus_tag="ACHOTW_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8686..9843)
                     /gene="acrE"
                     /locus_tag="ACHOTW_003994"
     CDS             complement(8686..9843)
                     /gene="acrE"
                     /locus_tag="ACHOTW_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP9846010.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2695 a         2594 c         2438 g         2380 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttatgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgact taggatgcgc aaaaaactca tccggtgcag
      541 cttgctccac tatttcgcca cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa
      601 accccatctc atgggttaca cacaacattg tcatacccga ttgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga tgtgggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgagcgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctggct atagcgcgac atgctgaaac agaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg cgggatcaac ggttgcttgc
     1381 tgaacgctac tgaaaagatc gaacaacccg ataatgatca ccaggctggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaacccaac gccagcgact ctgccgcttc atattgccct
     1561 ttaggcagcg cctgtaatcc acctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcagtttg tcgatactgg tgccttctgc cataaacaaa
     1681 ggcagcatga ccgaagacat aaacagaacg gtaatcagcg gcacgccgcg ccagaattcg
     1741 ataaaaatga ccgataagat gcgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaactgatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tcaagagcaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcactacc cagaccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgattgac acaaggccaa tcagtaatgc caagttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagtcca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccagttagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggcttgagt tatggctcgc cgggcgcgag ggatgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttccacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc
     2461 gtctggttca gcacggtgcc agcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggtgg aataatcacc
     2581 cgcagtgcct ggggaataat gacctggcgt agcgtaaccg ggttgggtaa tcccagtgac
     2641 cgagccgctt catgttgacc ataaggcact gcctggatcc ccgcgcggat aatctcggcg
     2701 ataaatgcag aggtataaac cgaaagtgcc agcgttaagg ctgccagttc aggaattaaa
     2761 accatcccgc cgcggaagtt aaagccttgt agggctggga catcccagtg tagtgctgcg
     2821 ccaaaaagcc attgcgccag taaaggcaaa ccgatgatca acactgcggc gatgggccag
     2881 gtgcggcgca gctgtccggt ctttatctgg tatgttttat taaagcggaa tagcccaaca
     2941 gaaaggacta tagccataac aacagccagg ataaacgcaa taaatccgtc tcccagctgc
     3001 ggtgacggaa tataaagccc acgattgctc aaaaaggcca gatcaaacgc gctcacagcc
     3061 tggcgtggtc cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc
     3121 gggggaatat tacggaagat ctcaatataa attgtggaaa gctttcgtag cagccagtta
     3181 tccgaaagtc tcgccagacc gataaagaag cccaaaacag aagcgaacac aatacacaat
     3241 gcagaaacca gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc
     3301 tgctggtaat cgatcaaatg ctggacaata ccgaagccag cgccgcgatc cagaaaggca
     3361 aaacctgaag taatgccacg attattgaga ttcgttacag tgttgtgaaa taaccaacca
     3421 acaatgccga caacagcaac aacggcaagg atctggaata accaggcgcg aaccgtaggg
     3481 ttggcaaaag agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa
     3541 agcataacgc tgaagagagc gccgccgtag cgacgctcgt tacctcacac cgacttaacg
     3601 cacgggcggt gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat
     3661 tttcagcggg ctttctgaac ctacgttacg ctcgaaaatt tccgagtagt tacccacctg
     3721 tttgatgatg ttataggccc atttattatc caacttcaga tccttgccgt aatcgccttc
     3781 tttacccagc agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcgacgtt
     3841 ctgggaattg atgcccatct cttcagcatt tagcatggcg aaaagcgtcc agcgtacaat
     3901 cgagaaccat tcatcatcgc cacgacgaac taccggccca agcggttctt tagagataac
     3961 ctccggtaag acaatccatt cagccgggtt gcttaatttg atgcgcaggg catacagttg
     4021 ggattgatcc gaagccagcg tatcgcagcg accggattcc agtgccttcg ctgattcgtc
     4081 agaacgatcg aaagtcactg gtgtgtactt catattgttt gccttgaaat agtcggcgac
     4141 gttgagttcg gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc
     4201 gctttttagc cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc
     4261 agtaaatgcc attcccatcc cggcatcgcg agatgaagtc caggtcgtat tacgggagag
     4321 caaatccact tcccctgact gtaaagccgt gaagcgttct tttgctgtga gtggggtata
     4381 tttcactttc gtgtcgtcac caaatacagc agcggcaaca ccacgacaaa catcaacatc
     4441 aatacctgaa aacttaccgt ctgcatcggc ataagagaac ccaggtaatc catcactgat
     4501 cccgcattgc acaaaacctt ttttctgaac ggcatcaagc gtcgcgccag catgcgcctg
     4561 atttgcaacg gcaagcagca cgctggcggc agccagtgtg gctatcatca tctttttcat
     4621 aatgcttcct gtgaggcgaa aattatcgtt ataggacgtt tgggaaacgt cttttgcctg
     4681 tctgtggcca tcctcttata agcaaaagta gtgccagctt ttgcgcacga tttcgcgtcg
     4741 caataagctg agtgtagaca ggaaataata aaaacagcgc cccgcaatgg tgcgaaaatg
     4801 aaacatgcgc catattttgg cgcaaaacat ttacgcgtgt tttactaagc agaataatga
     4861 ttgttagcgt gagtatattt ctggtgggaa gtgcgaaagt taaattgatg cgttatttcg
     4921 aaagcagcga aataacacga tataacatgc taaatcagca gagaattagc cagcagaata
     4981 tagcaatatt ttatcttatg taaagatact gtatcgttaa acagagcgcg aaactccgcg
     5041 ccctgttcag ttatttcgac agagcgatga tccccatcgt caggccagca acagccgctg
     5101 ccccgccagc aatagcccca gccgtttccc agttatcctg agtcgtgcct ttcatacagg
     5161 tattggtgtg aaccagacga ccctgatcgt cataaaccgg aacgcagggc gaatcgtgag
     5221 cacagcccgc cagcaatgcg gtgagcaatg ccacaggaat taatcttttc atggtgttac
     5281 ctcagtaata aacgctaacg cttaataagt taactctctt attaacgagc tgaatgccat
     5341 tttatcaccg gaggttataa atcttgcgga atgaataatg acaaattatg tgaattgtaa
     5401 aaatagtgaa gggagcggga gctatagaaa gcttttaaag agtgagcaaa agaatatctt
     5461 attaattttt atgggtttca ctggaaaaca taaaggcacc cgaaagcgcc tttatgtttc
     5521 agatttatcc tttaaagcaa cggcggatca ccacaaagaa caccggtacg aagaagattg
     5581 ccagcaacgt tgcagagacc attcctccca ttaccccgat acccactgcg ttctgcgcgc
     5641 cactgccggc accgttactg atagccagcg gtaatacgcc gagaataaag gcgagagagg
     5701 tcatcaggat aggacgcaga cgcatacgta ctgccattag tgtcgcttca acaacacctt
     5761 taccctcttt ctccatgaga tctttagcga actcaacaat caaaatagcg tttttggccg
     5821 acaagccaat tgtcgttagc aagcctacca taaagtagac gtcatttttt tgattaaaga
     5881 gtgtcgccgc cagcagcacg ccgacaatcc ctaacggcac gaccaacata accgagacag
     5941 gaattgacca gctttcatag agtgcagcaa ggcacaggaa aacaaccaca aaggaaattg
     6001 ctaccagagc gggagcctgg tttcccgata agcgttcctg atacgacata cccgtccagt
     6061 cataaccaat gcccgcaggt aattttgacg ctaggttttc catcaacgcc atggcatcgc
     6121 cggaactggt tcctggcgcg gcttccccct gaatctccat tgacggcaga ccgttgtagc
     6181 gttccagtcg cggagagcca tacacccaat gtgaagtggt aaaggcagag aatggcacca
     6241 tttcgccgtt ggtgctgcgg acataaagtt tatcgacatc ttctggcagc atacggaatt
     6301 tggcatccgc ctgaacatac acctttttca cgcgaccacg gtcgatgaag tcgttaacgt
     6361 aagtcccacc caacgccgtt gaaatggtct gattgatgtc agaaagtgaa acacctaatg
     6421 cctgcgcctt ttcctggtca acttccagtt taaactgcgc ggtgtcttcc aggccattgg
     6481 gacgcacgct gactaagctg gcaggatgtt gcgccgccat accaagcaac tggttacggg
     6541 cctgggttag ggcatcgtga cccagcccag ccttatcaat taactcaaag tcgaaacccg
     6601 ttgccgtgcc cagttcaaca atggctggca tattgaatgg aatgacaaaa ccgtcgcgga
     6661 tcttgcccaa ctccattttg gcacgatgga ttaccgcttc cgcactgttt tcatcaccat
     6721 tacgctcttc ccacggtttc agactgacga aggccatacc tgtagattca attggtcaac
     6781 gcaacagtta tgtgaaaaca tggggttgcg gaggtgcctg gccgctgaag ctaaagccgt
     6841 taaccgtaaa gacactttca acgtttgctt tctcgttctt cagatagtaa tccgtcactt
     6901 gatccaacac tttttgcgtc cgctcttgcg tcgcgccagc gggtaactga atcatggtca
     6961 gaaagacacc ctgatcctct tcaggtaaga aggaagacgg aagacgtaaa aacaacacca
     7021 ccattcctgc aacaatcagc gcatagatca gtaaatatcg tcctgtggaa ccgaggattt
     7081 tgccgacgct gttggtgtag tggttaacgc tatgatcgaa ggtggtatta aaccagccga
     7141 agaaaccacc cttattttcg tgatgctcag ctgaggtggg tttgagcagt gttgcacata
     7201 acgcaggggt aagaatcaat gccaccagaa cagaaagcgc cattgccgaa acgatggtga
     7261 tagagaactg acgataaatt gccccagtag aaccgccgaa gaatgccatc ggaataaata
     7321 ccgctgacag caccatcgcg atacccacca gtgcgccctg tatttgcgac attgattttt
     7381 ccgtcgcttc ttttggcggg agcttatcct ccatcatcac gcgctcgacg ttctccacca
     7441 ccactatcgc atcatcgacg agcagcccga tggcaagcac catcccgaac atcgttagtg
     7501 tgttgatgga gtaaccaaaa gcggcgagga tggcaaacgt ccctaacaac accacgggta
     7561 ccgcaatggt ggggatcagc gttgctcgca tattctgcaa gaacagatac atcaccagga
     7621 acaccagcat aatagcttcg aacagtgttt ttaccacttc gtgaatagaa agctggacga
     7681 atggcgtggt gtcataaggg tagagaacct tcattccctg cgggaagaat ggctgtaatt
     7741 ccgccagttt tgccttaatg gctttcgcgg tatcgagagc attcgcgccg gttgccagct
     7801 taatacccag gcccgccgcc ggttttccgt tgatgcgagc gataacgtta tagttttcac
     7861 cgccaagttc aacccgtgcg acatctttca gacgcaccac cgagccgtca ctgtttacgc
     7921 gcagggtcac tttgccgaat tcttccggat tttttaaccg cgtctgagca ataatcgagg
     7981 cattcaactg ttgccctggt aacgctggcg ttccgcccaa ctgtccggca gcgatctgag
     8041 cgttctgtac cttcaactgg ttaatcacat caaccggtgt cagtttatat ttgtttagca
     8101 gatcggcgtc cagccagata cgcatcgcat actgtgcgcc gaaaagctgt acgtcaccga
     8161 cgctattcag acggctaagc gtatctttaa cgttagaggc cacatagtcc gagatatcgt
     8221 cctgtgtggt gtctgggtta tcagagacaa agcctgccac catcaaatag ctgctactgg
     8281 acttttcaac actgatcccc tgctgctgaa cctcctgcgg cagcaacggc gtggcgagct
     8341 ggagtttgtt ctgcacctgc acttgcgcga tatcaggatc ggtcccggac tggaaggtaa
     8401 gggtaattgt cacgctaccg gcggaatcgc tggtggagga catatacatc aggttatcga
     8461 taccgttcat attctgttcg ataacctgcg tcaccgtatc ctgcacggtc tgcgcatcag
     8521 cgcccggata gtttgctgaa acagaaaccg caggcggtgc aatggttgga tactgagcga
     8581 cgggcaattg taggatcgcc agtgcgcccg ccatcatcag aataatggcc agcacccatg
     8641 caaatatcgg tcgtcgaata aaaaagtttg ccatgtcaga ttaccttact tcgatgcagt
     8701 atctgcgggg gtatcggtag tggctttaac ctgctctccc ggacgcgctt tttgcaggcc
     8761 gctgacaatg acttgatcgc cagatttcag accttcgcta atcaaccatt tatcgccaat
     8821 cgcctgactg gcaacgacag ggcgcacttc cacctgactt ttatcgttaa caatcagcac
     8881 ggttgcatca ccacgcggtg tgcggctaac gccttgttgc gggatgagaa tggcgtcagg
     8941 ttggacgcct tcatcaatcc gtgcacgcac aaacataccc ggcaaaagcg tatgttgcgg
     9001 gttagggaag acagcacgta gggttatgga gccggtgctt tcatcaacgg tcacatcgga
     9061 gaattgcagc gtacctttca ggggataggt ttgaccgttt tccatgacca actctacgtt
     9121 gctggtggcg ttttccttat gcaaatttcc ttgctctacg gactgcttca gcctcataaa
     9181 atcgttgctg gattgggtca catcaacata gataggatcg agctgctgga cagttgccag
     9241 ttcagtcgtt tgcccattag tgacaagagc gccttcggtc acagtcgatt tgccgatacg
     9301 tccgctaatt ggcgcagtca ctttggtata agcaagattg atgcgagcgc tttcgactgt
     9361 ggcttttgcg gcaatcacgg cggcatcggc ctggcgagca tcagcaatgg cctggtcgta
     9421 ctcctgctgg ctgatgtatt tcgtacccac gagcggaacg taacgtttta ccgtcaaatg
     9481 cgcgatggcg gcggcggctt cacttttcgc cagttcgcct ttcgcgctgt cataatttgc
     9541 ctgataggtc gcgggatcga tctggtacag ggactggcct gcttgcacat cgctgccttc
     9601 agtgaaattg cgattcagta cgatcccgct aacctgtggg cgaacttcgg ctatacgata
     9661 agcattggtg cggcctggta attcagtctt aacttctaac ggggccgttt ttacaatatg
     9721 aacggtaacc tgcggttcac cgacgtgagc tttctcttct cccttatcgt tacaaccggc
     9781 gattaaagcc gcggagatca gaataaagga gggcaggagg aaaaacctgg catgtttcgt
     9841 cattactatt cctcaaaaaa ccaaaagcgc gttatttacc caaaaaggca acgcgttaac
     9901 tcgcagaaag aaaaatacag ttcgctatcc tacaaattat cattcgtcga tgtaaggaat
     9961 agttatgaat acaggcgtct caaggcacat aaacacaaaa aaagattaat attctactgt
    10021 tttattttga cgcgggttga aagaggcaga attaaaaaat cgtaaattga agtatatatt
    10081 gatgtagtga atgtatctta agtaaat
//
LOCUS       DAZKIF010000148        10022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZKIF010000148 DAZKIF010000000
VERSION     DAZKIF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.149"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            177..329
                     /gene="hokA"
                     /locus_tag="ACHOTW_003995"
     CDS             177..329
                     /gene="hokA"
                     /locus_tag="ACHOTW_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP9846011.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(306..377)
                     /locus_tag="ACHOTW_003996"
     CDS             complement(306..377)
                     /locus_tag="ACHOTW_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846012.1"
                     /translation="MRNAVRKAGITFRRRLLLFQIAG"
     gene            complement(517..729)
                     /gene="cspA"
                     /locus_tag="ACHOTW_003997"
     CDS             complement(517..729)
                     /gene="cspA"
                     /locus_tag="ACHOTW_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP9846013.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1010..1300)
                     /gene="yiaG"
                     /locus_tag="ACHOTW_003998"
     CDS             complement(1010..1300)
                     /gene="yiaG"
                     /locus_tag="ACHOTW_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP9846014.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1734..2444
                     /gene="yiaF"
                     /locus_tag="ACHOTW_003999"
     CDS             1734..2444
                     /gene="yiaF"
                     /locus_tag="ACHOTW_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP9846015.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2494..3468)
                     /gene="ghrB"
                     /locus_tag="ACHOTW_004000"
     CDS             complement(2494..3468)
                     /gene="ghrB"
                     /locus_tag="ACHOTW_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP9846016.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3572..4231)
                     /gene="yiaD"
                     /locus_tag="ACHOTW_004001"
     CDS             complement(3572..4231)
                     /gene="yiaD"
                     /locus_tag="ACHOTW_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP9846017.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4384..6717
                     /gene="bisC"
                     /locus_tag="ACHOTW_004002"
     CDS             4384..6717
                     /gene="bisC"
                     /locus_tag="ACHOTW_004002"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP9846018.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6686..7126)
                     /gene="yiaC"
                     /locus_tag="ACHOTW_004003"
     CDS             complement(6686..7126)
                     /gene="yiaC"
                     /locus_tag="ACHOTW_004003"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9846019.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7123..7686)
                     /gene="tag"
                     /locus_tag="ACHOTW_004004"
     CDS             complement(7123..7686)
                     /gene="tag"
                     /locus_tag="ACHOTW_004004"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9846020.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7844..8542
                     /gene="yhjY"
                     /locus_tag="ACHOTW_004005"
     CDS             7844..8542
                     /gene="yhjY"
                     /locus_tag="ACHOTW_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9846021.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8771..9973
                     /gene="yhjX"
                     /locus_tag="ACHOTW_004006"
     CDS             8771..9973
                     /gene="yhjX"
                     /locus_tag="ACHOTW_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9846022.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2389 a         2442 c         2580 g         2611 t
ORIGIN      
        1 acaaacttat taagctagaa tagccacggg tgcttgagac tgtttgtctc aggtattcac
       61 cgaaaggcag acagagaaaa gccccacctg actataaatc aaagtgaggc ccctctatgt
      121 atagacacca taaggttagc ctcttactcg ttggaaatca acacaggggg ctgggaatgc
      181 cgcagaaata tagattgctt tctttaatag tgatttgttt cacgctttta tttttcacct
      241 ggatgataag agattcactg tgtgaattgc atattaaaca ggggagttat gagctggcgg
      301 catttttagc ctgcaatttg aaagagtaag agtcttcggc ggaaagttat tcccgccttt
      361 cttacggcgt tgcgcattct cattgcaccc aaatttattt ttcacaaaaa taatactaga
      421 ttttattacg cgatcgatta tttatttcct gaaaaacaaa taaaaaaatc cccgccaaat
      481 ggcagggatc ttagattctg tgcttttaag cagagattac aggctggtta cgttgccagc
      541 tgccgggcct ttagcgccgc tttcgatggt gaaggacact ttctgacctt cgtccagaga
      601 tttgtaacca tcgttctgga tagcagagaa gtgtacgaac acgtctttag agccatcgtc
      661 aggagtgatg aagccgaagc ctttgtcagc gttgaaccat tttacgatac cagtcatttt
      721 accggacata gtgtattacc tttaataatt aagtgtgcct ttcggcgata tggcgtgctt
      781 tacagatttt gaagcgttaa aggaatgtgc actacgaggg gtatcaacga taactcttga
      841 agggacttgc cttactacac tgctttaatg gtctgtacgt caaaccgttg atgtgcatta
      901 agccacgcat tggcgggtga tgcaacaatt atttttcata tttatgatta atcggttgga
      961 aaacggtcct gtcatcagga ccgtaaacag caataaagtg gataaaagtc tattccatca
     1021 actgcttact taatgccggg ttggcttgaa tcaaacgcat caattttagt tcggcacttg
     1081 aaggcttcac gcgtctggat tcccattcct ttaccatggc gactgatacg cccaaaaccc
     1141 gggcgaaatc atcgattttt aatcctgtcc cttttcgtaa ctgctcaatt tcggtacagg
     1201 acgttgttct gtgcgtcagg gtaattttct gcgtttcatc tttaaaaaca atctgttcca
     1261 ggctgctcaa cagctcatgc attggatctt tatattccat tgaaaactcc tcaaatcaca
     1321 ctgcgggatc gtgaattgca tcgaagctca ttaagaatag tcgggaaaag cagacacagc
     1381 ggggtgaagt cagggcatgc tctggctatg attcgattca gccagtcatt tagcgtgact
     1441 tatagccacc taacgatctg ataaatgccg atatatctct aaccactcct ggtttgtaaa
     1501 gatttcgaaa atctaacctt ctgcgaaggc gtaaatctct ggctacaaaa aaggcaacgg
     1561 caatatcatt ttttaacaat gaaacaacaa attaaccttg tttcattttc tccatgattt
     1621 taccaggaag attacgtaga aagggtatcc tgcaagcctg tcctggacta tccttgtcac
     1681 gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga aaggagtaag aaaatggcga
     1741 caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc gttattaatg gtagttagcc
     1801 tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc gtttatcgac ttcctgcaga
     1861 atacagtgat gcgtagcggt gaacgtctac caaccctgac tgccgatcag aaaaaacagt
     1921 ttggtccttt tgtctctgat tacgcgattc tgtatggtta ttctcagcag gtgaatcagg
     1981 cgatggattc cggtctgcgc ccggttgtag acagcgttaa cgcaattcgc gtaccgcagg
     2041 attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg ttcgctgggt gtgctggcgc
     2101 aacaactgca aaatgcgaag ttgcaggccg atgctgcgca ctctgcgcta aaacagagtg
     2161 atgacctgaa accggtcttt gatcaggcat tcaccaaagt ggtaacgacg ccagctgatg
     2221 cattgcaacc gttgattccg gcggcacaaa ctttcacgca acaactggtg atggttggag
     2281 actacattgc ccaacaaggt actcaggtga gctttgtagc aaatggcatc cagttcccga
     2341 cttcacagca ggcaagtgaa tataacaaac tgattgcgcc attaccggca caacatcagg
     2401 cgtttaatca ggcctggact acagcagtta ctgcaaccca ataaagagta aaagcccgag
     2461 cgattatcgg gctttactcc acgcagtcgc ggcttagtcc gcgacgtgcg gattcacaca
     2521 gttcttctca acctttcctt gtaacgcatc aatcaaatta tccacggcac aggcggccat
     2581 gccataacgc gtctcatggg tggcagatcc aatatgcggt actgcgacga cgttggccat
     2641 tgagagcaac ggcgaatcta cggaaagtgg ctcttgttcg aagacatcca gcccggcggc
     2701 gtgaatttcc cctttctgca atgctgcgat cagtgcattt tcgtcaacca ccgggccacg
     2761 tccggcatta atgaaaatgg cggaggattt cattttggcg aattgttctg cgccaaacag
     2821 atgatgcgtc tcatcagtta acggcaggat caggcaaacg aaatctgact cttgtaacag
     2881 tgtatccaaa tcgcagtagc gggcgttgaa gcgttcttct gcttctttat ggtggcggcg
     2941 cgcgttatag aggatgggca tgttgaagcc aaagtgcgca cgttgtgcca gcgccatacc
     3001 gatccgtccc atcccgacaa tgcccagtgt tttatggtga acgtcagtgc cgtaccagtc
     3061 cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt tctgctacct ccacaacccg
     3121 acgagcggta gacaacacca gcgccatcag cgtatcggcg acggtttctg ttaagacggt
     3181 tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca tcgacatcaa aattgtcata
     3241 gccgacggag atcgttgatg tggcacgcag tttcggcatt ttttccagca atgcggcatc
     3301 aacattctcg tttgaaccca gtaaaccttc agcttcggca aaaattgctg cattttgttc
     3361 gacggtttgt gggctgaggt ttgccacctg gtgaacggtg aaatgctctt gcaggcgttg
     3421 cagtaaatca tcaggtaagg ctttgtagag gataacggac ggcttcatgc ttctctccat
     3481 tcactgataa ggcgatcacc ttagcctgaa atatgatgaa ggaaaactga agaatgccag
     3541 gtgatttctg catcacctgg catgaaaggg attacagcgg gcttaaggta atttctacac
     3601 ggcggttttg cgccttacct tctgcggtgc tgttgctggc gattgggtta gccgggccaa
     3661 ggccctgagt acggatgcgg ctggcgtcca cgccctgggt gatcaacgcg ctggcaacgg
     3721 aatccgcacg ctgctgggag agacgcatgt tcagatcgtg accacccgtg ctgtcagtat
     3781 aaccaatcac gttaaccgcc gttttcggat actctttcag caccattgcc acgccggtca
     3841 gggtgttagc gcccgccggt ttcagggttg cgctgctgct gtcgaaggtc acattgttcg
     3901 gcatattgag gataatgtta tccccgctgc gggttacgct aacaccagtg ccgcgcattt
     3961 tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc cacgccgccg cccagagctg
     4021 cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc tttcttcgaa gaagagagcg
     4081 caccaatacc cgcgcccacg agagagccca gacctgcgcc gatagcagat ttacctgctt
     4141 cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac cgccagagcg ccactcacta
     4201 cggcggcaat aagataaaca cgtttcttca ttgttaatcc ttaataacct ttttattctt
     4261 tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca tgaagattat tcctgggaat
     4321 actcggaaat ttgtaagtaa tatttaactg ctcaatacat ctaacctttc aggagtcttc
     4381 ggtttgacca actcatcctc acgatattcc gttctgactg ccgcccattg ggggcccatg
     4441 ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg gcgcgttagc cacaggaatg
     4501 gaaaactcct tgcagagcgc ggttcgtgac caggttcaca gcaatacgcg ggtacgattt
     4561 ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc cgcaaggcat tcgtgggcag
     4621 gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc ttattcacca acaacataaa
     4681 cgcattcgtg aggcttatgg tccggcatcg atttttgctg gttcctacgg ctggcgttca
     4741 aacggcgtgc tgcataaggc ctcgacatta ttacaacgct atatggcgct ggcaggcggt
     4801 tataccgggc atctggggga ttattcgacc ggcgcggcac aggcgatcat gccgtatgtc
     4861 gtgggtggta gtgaagttta tcaacagcag accagttggc cgctggtgct ggaacatagc
     4921 gatgtcgtgg tgctgtggag tgctaaccca ctcaatacgc tgaaaattgc gtggaatgca
     4981 tccgatgagc aggggctttc ttacttttct gcactgcgtg acagcgggaa aaagctgatc
     5041 tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat ggagtgggtg
     5101 gcaccgcaca tgggcactga tgttgcgctg atgctgggga tcgcccatac gctggtggaa
     5161 aatggttggc acgacgaagc gtttctggcg cgttgcacca ccggttatgc cgtcttcgcc
     5221 tcttatttgc tgggcgagag tgacggaata gcgaaaaccg ccgaatgggc agcagagatt
     5281 tgtggtgtta acgcagagaa aatccgcgag ctggcggcta ttttccacca aaataccacc
     5341 atgctgatgg ctggctgggg aatgcagcgc caacagtttg gtgagcaaaa acactggatg
     5401 atcgtcacgc tggcagcaat gttggggcaa atcggcacac ccggcggcgg ttttggtctt
     5461 tcttaccatt ttgccaatgg tggtaacccc acgcggcgcg ctgcggtact ctcttccatg
     5521 cagggcagct tgccgggtgg ctgcgatgcg gtagataaaa tccctgtcgc ccgcattgtt
     5581 gaagcactgg aaaaccccgg tggcgcatat caacacaacg gtatggaccg acatttcccg
     5641 gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca ggataccaat
     5701 cgcctgatcc gtgcctggca aaaaccggag ctggtggtga tctctgaatg cttctggacg
     5761 gcggcggcaa aacacgcgga tatcgttctg cctgcgacta cctcttttga gcgtaatgat
     5821 ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaagca agtggtgccg
     5881 ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttaagtga acgctgggag
     5941 aagggcggtt atgcacgttt tacggaagga aaaagtgagc tgcaatggct ggaaacgttt
     6001 tataacgttg cccggcagcg cggggcaagc cagcaggttg aattgccgcc atttactgag
     6061 ttctggcaag ccaaccagtt aattgagatg ccggaaaacc cggacagcga gcggtttatt
     6121 cgcttcgctg atttttgccg cgatccgcag gcgcatccat taaaaaccgc cagcggtaag
     6181 attgaaatct tctcgcagcg tattgccgat tacgcttatc cggattgccc ggggcatcca
     6241 atgtggctgg tgccggacga atggcagggc aatgccgaac cggaacagtt acaggtactt
     6301 tctgctcatc cggcgcatcg tctgcacagc cagctgaatt acagttctct gcgcgaattg
     6361 tacgcggtgg caaaccgtga gcctgtcacc attcatcctg acgatgccca ggcgcgcggc
     6421 atacaagatg gcgatactgt tcggctgtgg aacgcacgcg ggcaaattct tgccggagcg
     6481 gtcattagcg agggaattaa acctggcgtg atttgcattc acgaaggggc atggccggat
     6541 ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct gaccaaagat
     6601 ctccccagct cgcggctggg gaatggctgt gcgggtaata cggcgctggc atggctagaa
     6661 aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag ctcataatcc
     6721 aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa ccctgcgcct
     6781 ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga tggggatagc
     6841 gctgctgcac atactgcatc agcgccttac caataccgcg tctgacggcc ttcggtgcga
     6901 caaacattgc tgccagaaat cggccttcca taatgctgac aaaaccgaga agcttaccgt
     6961 cttcttccca gacccagttt ttcgcattgg caagatacgc atcccgcacc agcggaatgc
     7021 actcgcgcca gtaattcact tttataaagg gatgccccca ggttgtactt tccagccaca
     7081 gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt tatttcccgg
     7141 atagcaacag cagccaacca catgatcatt caccagccca catgcctgca taaaggagta
     7201 acagattgtg gtaccgacaa acttaaaacc acgttttttc agtgccttag atagggcgtc
     7261 ggaggcgggc gtagatgtgg ggatttcgct caacgttgtg gcttgtgtca cctgtggctg
     7321 atgatttaca aacgaccaga caaagtcggc aaacggttcg ccgttctgtt ccatttgcag
     7381 gtacgcccgc gcattaccaa taattgcctg aattttcccg cgatggcgga taatcccggc
     7441 gtcctgtacc agtctttcga catcctcttc ctgcattgcg gcaaccttca ccggatcgaa
     7501 ctgatgaaag caggcgcgat agttttcgcg ttttttgagg acggtgatcc acgataaccc
     7561 agcctgctgc ccttccaggc agatcatttc gaacagtttt ttcctgtcag tttcaggcac
     7621 gccccactca ttatcatggt aggcaatata aagcgggtcc tgactcaccc agccgcaacg
     7681 ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt ttcttgacgc
     7741 atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct tcatacaatg
     7801 acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca taaaaaaaag
     7861 cggtggtcgc tggcagctaa gcctgctggc gagtgtggta atcagtgcct tttttctcaa
     7921 cacagcttac gcctggcaac aagaatatat cgttgatacg caacccgggc attccacaga
     7981 gcgttacacc tgggatagtg atcatcaacc tgattacaac gatattttgt cgcaacgtat
     8041 tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa cccctgtgga
     8101 tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg ttacaactgg
     8161 cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg agtttggcga
     8221 cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtacct taggctggcg
     8281 tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata accagcaatt
     8341 tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga caaaccagaa
     8401 cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa atattgccgc
     8461 ctatgccgcg ctatctcagg cagaaaatac cactaacaat agcgattatc tgtatacgat
     8521 gggggttagt gccagatttt aacgtaacaa tcacaattga aaccattgaa taacaatagt
     8581 tgtggcgata gtgggtgcta acttaccaaa taataaattt ggtgaataat tgtcgcgtca
     8641 ttcattcctg aactaaggca tttcattccg ttctgatggc atttcatgcc gtttttcccc
     8701 aggcataaag tgcacttcgt tatggttgtc ggcagagatt tttccttttt attactgcag
     8761 gaatactgcc atgacacctt caaattatca gcgtacccgc tggttgacac tcatcggtac
     8821 tatcattacc cagtttgcgc tggggtcggt ttatacctgg agcctgttta acggcgcgct
     8881 ttccgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct tgttaagtct
     8941 ggggctggca atatcgtctt ctgttgcggg caaattgcag gaacgttttg gtgttaaacg
     9001 cgtcaccatc gcttccggca ttttgctggg attagggttc ttcctgacag cgcattctga
     9061 caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg atggcgcggg
     9121 ttacctgctg acgctctcta actgcgtgaa atggttcccg gagcgtaaag gtctgatctc
     9181 cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta tcgacacgca
     9241 gctgctggaa acggtcggtc tggaaaaaac ctttgtgatt tggggagcga ttgcgctggt
     9301 gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag tgaaaaccag
     9361 caatggtgtg gtggagaaag attacacgct ggcagagtcg atgcgtaaac cgcagtactg
     9421 gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga ttggggtagc
     9481 gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tctgcagcca atgcagtcac
     9541 tgttatttcc atcgccaacc tttcaggtcg tctggtgctg ggcattctgt ctgacaaaat
     9601 cgcccgtatc cgtgttatca ccattggtca ggtaatttcg ctggtgggta tggcggccct
     9661 gctgtttgca ccattgaatg cagtgacgtt ctttgcagcg attgcctgcg tggcatttaa
     9721 ctttggcggc actattaccg tctttccgtc actggtcagt gagttcttcg gcctcaataa
     9781 cctggcgaaa aactacggtg tgatttacct cggtttcggt atcggtagca tttgtggttc
     9841 gattatcgcc tcactgtttg gcggcttcta tgtgaagttc tacgtcatct ttgctctgct
     9901 gattctgtca ttggcgcttt ctacgacgat tcgtcagcca gagcagaaag tattgcgtga
     9961 ggcgcatatt tagccagaaa ctacaggttt tgaacgaaag ggggaggaaa tcctcctttt
    10021 tt
//
LOCUS       DAZKIF010000149         9948 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZKIF010000149 DAZKIF010000000
VERSION     DAZKIF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.150"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1051..1581)
                     /gene="yodB"
                     /locus_tag="ACHOTW_004007"
     CDS             complement(1051..1581)
                     /gene="yodB"
                     /locus_tag="ACHOTW_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP9846023.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1924..2574)
                     /gene="zinT"
                     /locus_tag="ACHOTW_004008"
     CDS             complement(1924..2574)
                     /gene="zinT"
                     /locus_tag="ACHOTW_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFP9846024.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2831..3466)
                     /gene="msrQ"
                     /locus_tag="ACHOTW_004009"
     CDS             complement(2831..3466)
                     /gene="msrQ"
                     /locus_tag="ACHOTW_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP9846025.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(3467..4471)
                     /gene="msrP"
                     /locus_tag="ACHOTW_004010"
     CDS             complement(3467..4471)
                     /gene="msrP"
                     /locus_tag="ACHOTW_004010"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP9846026.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4579..4992)
                     /gene="hiuH"
                     /locus_tag="ACHOTW_004011"
     CDS             complement(4579..4992)
                     /gene="hiuH"
                     /locus_tag="ACHOTW_004011"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP9846027.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5125..5796
                     /gene="hprR"
                     /locus_tag="ACHOTW_004012"
     CDS             5125..5796
                     /gene="hprR"
                     /locus_tag="ACHOTW_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP9846028.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5796..7154
                     /gene="hprS"
                     /locus_tag="ACHOTW_004013"
     CDS             5796..7154
                     /gene="hprS"
                     /locus_tag="ACHOTW_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP9846029.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(7262..8113)
                     /gene="hchA"
                     /locus_tag="ACHOTW_004014"
     CDS             complement(7262..8113)
                     /gene="hchA"
                     /locus_tag="ACHOTW_004014"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP9846030.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(8705..9891)
                     /gene="ompC"
                     /locus_tag="ACHOTW_004015"
                     /pseudo
     CDS             complement(8705..9891)
                     /gene="ompC"
                     /locus_tag="ACHOTW_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
BASE COUNT         2748 a         2047 c         2288 g         2865 t
ORIGIN      
        1 tgtttgtcaa gtgattatat gagaaagact tggtttattg ttgattaatg gtatgacctc
       61 cgattttcat tgatagctta tgtgaaattc atattgtgct tgactttcga agtgattgag
      121 ctcagtgacg agatttattg gaattatagt taaacagaag agaggttaaa taccgtggag
      181 agctcgagtt atacaaggcg ttaacgtgag caataaatag gatgcgaagt aattttggtg
      241 gctcggaaaa ggcgttttct gtcgttatca attgttcggg tgcagtttta tttttaacct
      301 gtttgcttgg aatattctga ggttgtttga tgctaaccat ctgatttttc aatcctaaat
      361 atagaatatt tattccaaat aaaacacata atatattgat tttattaacc catagcatag
      421 gtctcgaaaa ccggagtagg ggcaactcta ccgggggttc aaatccccct ctctccgcca
      481 ctttatcaat gacttatctc ccgacttccc gccttgcttt tcctaaacag aacaatcgta
      541 gaatattctt gaagggttag atcgtcactg ttttctgttc gatactgtga cattcagcac
      601 ttgatttgct atggatctga caggaagctt tcgagcgaaa atgtgcagtt attcagtcgt
      661 tttcttatcg ctcaccatta ttcttttaga cattcatcct atgaaactgc cgcaaagttg
      721 gtggtgggaa ctgaagttgc gtagagaagg ggtcaagaat tgagtcaaaa aaggaacgcg
      781 gccgtcagat taaagccgcg ggacaaagtc cataatttcc aagagttgtt gaatttccca
      841 gcttttttga aagcctttgt atcatactaa caagtaaaaa aggtttaaat accaaaatta
      901 acctgtattt ttgataagtc ttaccattgt acgatttaca cattttctgc tcctccttgt
      961 tgatagtgga aagagtgcgg tatgcacttg cgtaaacatt tatgatgcgt tgatgtccat
     1021 ggttcacatt tcctttgtca gaggatcgac ttatttgcgg cgaggcatca ttcgtaacag
     1081 agtattatct ttctgaatgt agtgatgaaa gagtgctgcg ccagcgtgag ctacgattaa
     1141 aaagtagcct atatttgccc aggtttcgtg aatatttttt atcagtgctt taatttcgct
     1201 gtttggggta acaaagggag acacattgaa accaaggaaa ctccacgatt ttccactgta
     1261 agccatcaaa gcaatcccca gcagaggaag cgcaaggaag gttatataaa gcatgatgtg
     1321 cattagcgaa gccgctttca tttgccaggc aggtggcggt ggcacaatag aaggatcact
     1381 ataacggtgt tttataatca ggcgtgaaaa cattaacacc caaacgaata tacccgcatt
     1441 gtaatgtgtt tctcgcatca gcagataagt actactacct ttaggaaacc agccacggag
     1501 ttccatcgcg gcataggtta ttgcaacgaa aagcagcgtt atccaatgta aataaatttg
     1561 agtttttgag aatcggttca tttttgtagc tcctgtaatt ttcacagtca agctatccca
     1621 cgaaccttaa aaattcctta tacagatgtg gttttaagaa tttgttattt aaaaaattga
     1681 tagtgcacat tgtaaccagc caatataagc aatggcgtga gcacaggtat ctttctctgt
     1741 tggccgtatt gtgtatggaa tccgttattg gttaactcaa tgcgggttag cttattaata
     1801 tacagacacg tcatagtgat gagcgtgtct gtagctagct tgcgttcagt ggcgtttgtg
     1861 gatggaccag tggcagttat gatgacatac taaagcggca tcgaggcgtt atcatgagaa
     1921 tactcaatga gacatcattt cctcgaccac ttcttcgcta ctcaactgat atggataata
     1981 cgttggccag ttttccattt catttaataa agattgctgt gaatcattac ccataaaaat
     2041 gtgaaaatga gatgattttc gtggtgcaat tatatgatcg ctaaattgta tatatttagg
     2101 ggctttgctt tcaggatctt tacattcgaa taagtaacga acgcctttct tgcctgattt
     2161 ataggtgagt attttgtatc catcgtaatc atatttacag gatgttgttt cattatttct
     2221 atggaattca acaatgccgt cctcaatgcc aatcatctcg atatctgttg catagccttt
     2281 gtgataataa tctttaattt cagcaaatgt tttagtttta tctgcatccg ctttcttctg
     2341 aaagacgggg gcaagtttgc cactttgcag taaaggataa acggattgcc agactccatc
     2401 ccagtcactg agcgttcggt tttgtacatt ggcatcatca aaaacaccat tagcagcttt
     2461 ttgttcgacc tctgttaagg gtttgccgtg tgagtgatga ccatgcgaaa aggcaggagc
     2521 gctaacaata aagacaccta aagcaacagc cagtttgtga agacgaatcg ccaaaacagt
     2581 ttcctccaga gtcattgtat atgatgtgta atgttatatt gtaacatatg acaaaatgca
     2641 acaactctta ggagatgaga gcatagtttt ctcaggtgta cttatctatc ttattgaaat
     2701 aaaatgaatt atgttgataa tttgaggttg atgacaaacg atacataacc tgatgtgatc
     2761 tgcgtaggcc ggatagggcg ctcgcgccac atccggcagg gataacgcgt actttgtcgg
     2821 caatatgaag ttatacagat aatttgttgt gaacctgctt gcgtaaccgg ttaaaaaggg
     2881 aacgcgactt cttataccgt aaggctaaaa gcagtacagc cagcccagcg tagatgagcg
     2941 gctgcggtga aataatcttc actgaccaca gataatgtat tggggccagg atcgcgacaa
     3001 gatagacgaa attatgcaac tgttgccagt gcttgccgag ttttcgctgc atcgcctggg
     3061 ttgaagtaaa cgctaaagca agcaaaataa tccagctgat aattcctaac gttaaataag
     3121 gccgggtaat taactccttg cctaataacg ccagattatt cacgcccagc tccagcaatg
     3181 cgtaactggt taaatgcagt gtcgcccagg caaagcacca taagcctaac aggcggcgtg
     3241 tgcgtatcaa taacggctgt tttgcgtagc gtgccagagg tgtgattaac aaggtcgcca
     3301 gcaaaaattt cagtgcagtg cgaccggtaa aatgctgaat atctttcacc ggatcggcac
     3361 ccagtccacc gtgattgatc gcccagacca gccagagaaa cggcaacaat ccggcaagat
     3421 gcaggcaaac tttcagccat gtcacctgtt ttgctgtcag acgcattcag aaattctccc
     3481 gcaaatccag gccacgatac agcgatgcca cctggtcggc gtaaccatta aacagtagcg
     3541 ttggctggcg ctgtacatcg aggatgccgc ctgaaccaat aaatcgttcg gtagcctgtg
     3601 accagcgtgg gtgatcaaca tgcggattaa cgttggcgta aaaaccgtat tcgtcaggcg
     3661 ctgccagatt ccaggtggtt ggcggacgct cgcgggtcag cttaatactg acgatcgatt
     3721 taatcccttt aaagccatat ttccacggca caatcagtcg caccggcgcg ccattttgtg
     3781 gcggtaacgc cttgccataa acacctacgg tcatcagtgt gagcggatgc attgcttcgt
     3841 cgagacgcaa tccttcgaca taaggatatt tcagcccgcc gccgataaag cggtcctgct
     3901 ggcctggcat ctgttccggt gcataaattg tttcgaaagc gacatacttc gcattgctgg
     3961 tgggttcggc aagcgccagc aatttgtgca gcggaaaacc aatccacggc accaccatcg
     4021 accacgcttc cacgcagcgc atacgataaa tacgctcttc cagcgggaag cgacgggtta
     4081 aatcatcgtg atcgagggtc aatggttttg ccacttcgcc gctgattttc agtgtccatg
     4141 gatcggtttt caggctacca gcattagcgg cgggatcggc tttatccagc ccgaattcat
     4201 agaagttgtt ataaccggag actttatctg ctggcgtcag tggcaggtta ttttgccagg
     4261 cggcaggctt gctgaactcc agcgcttttc cggcgggggc gggtgggcga tcgttccctt
     4321 taaaccagct aagcagatcg gcatgcgcag cgtgaggcaa agaaagtgca gctgcgctga
     4381 tgcccagtgc ttttaacacc tgccgacgct tcataaagaa taccgactcg gccgtaacat
     4441 ctgattcttt taaaaattga ttctttttca taagccatca cactttttgt cgttagccag
     4501 caagagccgt ttactgttaa tctgtcaggg agtgtcctcc ctaacagtag cagagatctg
     4561 cttttacata tttatgcatt aactgccacg ataggttgaa tacccatatt ggctaagtaa
     4621 taaaggcaca tgataatgct cgttcacttt attaatatga aattcaacgg ggatctcagg
     4681 gaagaaactt tcaagatttt gtttcttgaa atagtccccg gttttaaata cgacacggta
     4741 atcgcccgta gttgcagttt gctcgggcca cagtgcctta attcgtccat ccttatctgt
     4801 tttggcggta ttaagttgta accagccgtt gtccgctttc ttttcaagag tgactgtcac
     4861 gtcggcagcg ggttttcctg tttgctggtt caaaatgtgc acgctaagaa tgttttgttg
     4921 tgcagcatag accaaagaag gtaatgaaaa tgcagccgtt actacggaga gtactaaata
     4981 acgctttaac atgtttatat ccttgtcatg tgaatgagtt ttgaggatag cgaaaaagcg
     5041 tccagggtaa ccttacagca gcattacaat tttgtaatga accaggctgt ttctatgaca
     5101 tatgatttat ggcatattat tttcatgaag attttactta ttgaagataa tcaaaggacc
     5161 caggagtggg taacgcaggg gctttccgaa gcgggttatg tcattgatgc cgtttctgat
     5221 ggcagagatg ggctttatct tgcgctgaag gatgattatg cattgatcat tctggatatt
     5281 atgcttccgg gtatggatgg ctggcagatc ttacaaacgt taagaacggc aaagcaaacc
     5341 cctgttattt gccttactgc aagggattct gtcgatgaca gagtcagagg gctggacagt
     5401 ggagcaaatg attatctggt aaaacctttt tcattttctg agttgctggc aagggttcgg
     5461 gcacaattaa ggcaacatca cgctttgaat tcaacattag aaatcagcgg cttaagaatg
     5521 gactctgtta gtcaaagtgt gagcagggac aatatcagta ttacactgac gcgcaaggag
     5581 tttcagttac tttggctact ggcctccaga gctggcgaaa ttatacccag aacggttatt
     5641 gcgagtgaaa tttggggaat caactttgat agtgatacca atacggtgga tgtcgccatt
     5701 cgcaggctcc gcgcaaaagt tgatgatcct tttcctgaaa agctaattgc cacaatccgt
     5761 gggatgggat attcgttcgt agcggtaaaa aaataatgaa aagattatct ataaccgtcc
     5821 gtttaacctt gctttttata ttgctgctgt ctgttgctgg cgccggaatt gtctggactc
     5881 tctataatgg cctcgcaagt gagttgaaat ggcgcgatga tacaacactc attaaccgga
     5941 cagcgcagat caagcagtta ttaattgatg gggtaaatcc agatacgtta cctgtatact
     6001 ttaaccggat gatggatgtt agtcaggata tcttgatcat tcatggtgat ggcatcaata
     6061 aaattgttaa ccggacaaat gtcagtgatg acatgttaaa taacatacct gctagtgaga
     6121 caatcagcgc agctggcatt tacagaagca ttattaatga tacagagata gatgctttac
     6181 gaattaatat tgatgaagtt tcgccatcat taacggttac tgtggctaaa ttggcttcag
     6241 ccagacataa catgcttgaa cagtataaaa tcaatagcat tataatttgc attgtcgcca
     6301 ttatactgtg ctcagtatta agtccgctgt taatccgaac ggggttacga gagatcaaaa
     6361 agttgagtgg tgtaacggaa gcgctgaatt ataacgatag ccgggagcct gttgaggtta
     6421 gcgcattacc gagagaacta aaacctcttg ggcaggcgtt gaataaaatg catcatgctt
     6481 tagtcaaaga ttttgagcgt ctaagtcagt ttgctgacga tctcgctcat gaacttagaa
     6541 cgccaattaa tgcattactg ggtcagaatc aggttacgct cagtcaaacc agaagtatcg
     6601 ctgaatatca aaaaacaatt gccggtaaca ttgaagagct ggaaaatatt tcgcggttaa
     6661 cagagaacat actgtttctt gccagggcag ataaaaacaa tgttttggtg aaactggact
     6721 cgctttctct caataaggaa gtcgaaaatt tgttggacta tcttgaatat ctttcagacg
     6781 agaaagagat ttgctttaag gtcgagtgca atcagcaaat ctttgcggat aaaattttac
     6841 tgcaacgaat gttatcgaat cttattgtta acgctattcg atattcgcca gaaaaatcgc
     6901 gtattcatat aaccagtttt cttgatacca acagctatct taatattgat atcgccagcc
     6961 ctggagcgaa aattaatgag cctgaaaaac tcttccgtag attttggcgg ggagataatt
     7021 cgcgtcattc cgtaggtcag ggacttggtc tttctttagt caaagcgatt gccgaattgc
     7081 atgggggaag tgctacgtat cactatctca ataagcataa tgtgttccgg attacgttgc
     7141 cgcaaagaaa ttaacatagg gcttcagtac gccataaatt aaccgtgaaa aaacgcagcc
     7201 acgtcgtgag tactaacgcg gctgcgattg attatgcgct tacattcaaa cgtaacaggg
     7261 attaacccgc gtaagctgcc agcatttcct gcgccgccag tttacccaac gcattcgctg
     7321 caaaaggact gtcgccggtg agaagtttac ggtccttatg tactcgcccg gtgatgtcgt
     7381 cattaatgat gttcatgccc attttcttca gttcttcgcc gaagtaccag gtgagatgac
     7441 ccggcatata gccaatctct ggcgtttgtt tgtctgcggc gtctgggaat gcgcaaatgg
     7501 aataaccatt cagtggatta tcgccgtggc gaagcgccag aaaagccgcc gggccgtggc
     7561 aaagggagat aacaaaacgg tcatttttga ttgcccactg taaagcggca gctacgtcct
     7621 ggctttcagg taaaccaata agtgcgccat gaccaccagg aacaaagatt gctgcatatt
     7681 cgctatcagc gttgaggctg gcgacaacat ccgcgagttt cttcggattg cggaacaacg
     7741 atttatgctg ctcaaagaat ggcattactt tttcatcttt ctgcggcata gcccagtatt
     7801 caaacttggt catcagaccg gaaatggtcg ccacttcgaa ttcgaaacct gcagcatgga
     7861 gatgatacaa cggcagcaac gtttcaatcg gatggttacc ggtcgagaac agttttccgt
     7921 tatcggtcgg caaataacgt tcgtccgcgg cgatcaccag aattttatgt ttaccgcgat
     7981 aaggttttgg atagtccacg ccatcaagat cagagacagg actggtatat tggctaagcg
     8041 aatattctga agggaagaat gcattatctt cagcaatatc gacctgcgga tttttactgg
     8101 tttgaacagt catagtgtat tccttattgt tgcttagtta gggtagtcac tatatttgcg
     8161 actgagctgg tcgctatttt taaccaggct ggaaaattga gtcaatatat cgaaagcggt
     8221 agtcggcggg tgtaagtatg taatgatgat gctgcactgg tgttgaatat tattcacgcc
     8281 aaattttcct tattgagcaa cttcgtcgca agtggcctca atgattttag accattatca
     8341 gaggtacggg gcggcgggga gagatttagt gctgtggaat attgtggtgc tgatgaatat
     8401 ttatatcgcg gattggtaga gaaaaagctt atttcatgca ctatttttgc aaatatttct
     8461 atatgggaag cgtgtttgtt acggattaat tcataaagaa atgcatttgg gtgttggctt
     8521 gaagagtcgc tggttgatgt tattgtgcca ggaggggcag ggtaaggggg tgtatcgata
     8581 agctttggca attgcgtgtg ccgcagatgg agatgaaatg ccaataaaaa agcctgagtt
     8641 cgatgaggga cgaactcagg ctagtgttca gttttacaat aacacgccgc ggcgcgttgg
     8701 ctgattagaa ctggtagact aagcccacag ccacgatatc atcggtagcg atgccgtttt
     8761 ctttgtagaa gtcgtcgtcg ttgtccagca ggttgatttt gtaatcaaca taagtggaca
     8821 tgtttttgtt gaagtagtag gtcataccaa tatcaacata tttaaccaga tctttgttgg
     8881 tgtaatgatg attaccctgg ctgtcgaagt tatcactatt tacaccgcct aaatccttac
     8941 ctttagattg cagataagcg atggacgggc gcagaccgaa gtcgaactga tactgagcaa
     9001 caacttcaaa gttttgggtt ttgttagcga tggcatcagc atcaccgtaa ccagtcatgt
     9061 tacggggttc agcatacata gctgctaagt aaacgttatt agcgtcgtat ttagcaccaa
     9121 cggtccatgc ttctgctgtt tcaccgccag cagttgcatc gaagcgatct ccataacgag
     9181 tgttatgagt acctttgtga acctgattgt cggtacggtc agagttagag taagcagcgc
     9241 ccagacttaa accaaagtca aagtcatagg aagtggacat actgaaccgt cgccgttctc
     9301 tttagcaagt ttacgtccat taccgttacc tgaaccttct tgatcaaata atccgctttt
     9361 ctcgttgtta ccctgatact ggagtgcaaa gttcagacca tcaacttgac caaagaaacc
     9421 attgttacga taggttgcta cgctgttagc acgaccgttc atgaagttgt cagcgccagc
     9481 ataggtatct ccaccaaatt ctggcagcat atcggtccat gcttctacgt catatacgac
     9541 accgtagtta cgaccatagt cgaaagtacc attctgacca aagccgaaac ctgcaaatgc
     9601 cagacgcgtt gcggtattac ccttgtcgct ttcaggaccg tttgcgccaa tgttaaattc
     9661 ccactgacca taaccagtga attggtcagc gatctgagtt tcacctttga aaccgatacg
     9721 ggcatatgac atgtcgccat cgctgctagc atcatcagag aagtagtgca ggcccgctac
     9781 ttttccgtac aaatccagtt tattgccatc tttattataa atttcagccg catttgctgc
     9841 gccagcaact aataacgccg ggaccagcat tgccagaact tttcttttca ttatgtattc
     9901 ccttgtgatt ataatcttca tgaatatatc aataagtgcc gttatcca
//
LOCUS       DAZKIF010000150         9826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZKIF010000150 DAZKIF010000000
VERSION     DAZKIF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.151"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..243
                     /locus_tag="ACHOTW_004016"
     CDS             <1..243
                     /locus_tag="ACHOTW_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9846031.1"
                     /translation="LAVPPAYTSQRCACCGHTAKENRLSQSKFRCQACGYTANADVNG
                     ARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            287..394
                     /locus_tag="ACHOTW_004017"
                     /pseudo
     CDS             287..394
                     /locus_tag="ACHOTW_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(378..1016)
                     /gene="ytfB"
                     /locus_tag="ACHOTW_004018"
     CDS             complement(378..1016)
                     /gene="ytfB"
                     /locus_tag="ACHOTW_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP9846032.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1234..1854
                     /gene="fklB"
                     /locus_tag="ACHOTW_004019"
     CDS             1234..1854
                     /gene="fklB"
                     /locus_tag="ACHOTW_004019"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9846033.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2163..3575
                     /gene="cycA"
                     /locus_tag="ACHOTW_004020"
                     /gene_synonym="dagA"
     CDS             2163..3575
                     /gene="cycA"
                     /locus_tag="ACHOTW_004020"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP9846034.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3620..4282)
                     /gene="ytfE"
                     /locus_tag="ACHOTW_004021"
     CDS             complement(3620..4282)
                     /gene="ytfE"
                     /locus_tag="ACHOTW_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP9846035.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4390..5355)
                     /gene="ytfF"
                     /locus_tag="ACHOTW_004022"
     CDS             complement(4390..5355)
                     /gene="ytfF"
                     /locus_tag="ACHOTW_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9846036.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5463..6323)
                     /gene="qorB"
                     /locus_tag="ACHOTW_004023"
     CDS             complement(5463..6323)
                     /gene="qorB"
                     /locus_tag="ACHOTW_004023"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9846037.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            6412..6792
                     /gene="ytfH"
                     /locus_tag="ACHOTW_004024"
     CDS             6412..6792
                     /gene="ytfH"
                     /locus_tag="ACHOTW_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9846038.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6910..8853)
                     /gene="cpdB"
                     /locus_tag="ACHOTW_004025"
     CDS             complement(6910..8853)
                     /gene="cpdB"
                     /locus_tag="ACHOTW_004025"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP9846039.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            9043..9783
                     /gene="cysQ"
                     /locus_tag="ACHOTW_004026"
     CDS             9043..9783
                     /gene="cysQ"
                     /locus_tag="ACHOTW_004026"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP9846040.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2230 a         2504 c         2598 g         2494 t
ORIGIN      
        1 cttgctgttc cgccagcgta cacaagccag cgttgcgcgt gctgtggtca tacagcgaaa
       61 gaaaatcgcc tgtcacaaag taaattcaga tgccaggcat gtggatatac agcgaacgct
      121 gatgtaaatg gcgctcgtaa cattttagcg gcggggcacg ccgttcttgc ctgtggggag
      181 atggtgcagt caggccgccc gttgaagcag gaacccaccg aaatgattca ggcgacagcc
      241 tgaacgtagc agggatccac gtccttcagg gcttggagga tgtcaacaca tcagtatggt
      301 ttcaccccag gcactggaag ccagacgtaa ggtcatcaaa gagacaatcc ttagctggct
      361 tttggttgac ccttcatcta ccgcgcacga ataaaactgc catccggctg gcgggtgaac
      421 aggacctgtt gattattccc cgtatcaatg gttaagcccg tcaccacgcc actggcgttc
      481 tggcggattt tcaccatctg cccgttttgc aaattactca gcggcttacc cgcgccttct
      541 acctgcgcca ttgcatagac atcggtagcg ggcagtccgt ggtcgcggaa cagctgcgcc
      601 atcgttttac cgggttcgac acggtaagag cgccattgat tatcaattcc gctatctggc
      661 tggaatggtt gcgtttgggt agttttcggc tgttcttctg gctggccttc ctgtataggt
      721 tctggagcga ccggagccac ctgatcagga taattttgtg gggtgactaa ttgcgcccgc
      781 agctgttctt ctgtcggcgg ttgtgattgc gactgaatat ctaactgcgc ttcacgcgtc
      841 accaccggcg cgttgggcgt atcatcagat ggaagcagaa atccgaccac caatacaatg
      901 gcggcaataa tgatcccccg acgatgcatc ggcggcagcg ggtccatgaa gcgaaaattg
      961 tccggtgcgt gccagacttt ctccagggtt ggttttagtt caaagcgccc gggcatggtt
     1021 tcctcctgct ccgcgtcttg ttcctcaatc atagcctatg aataagctaa cgctatgatg
     1081 tccgtggtaa acccgccttt attatattca tacgggatat tgctattgtt tctttttccc
     1141 tgggatttgt catcattccc gcgacaaagt tttacccaaa gaagtgtggc tgatatgctg
     1201 cccgctactt tataccctaa gaaaggaaat acgatgacca ccccaacttt tgacaccatc
     1261 gaagcgcaag caagctacgg cattggtttg caggtagggc aacaactgag tgaatctggc
     1321 ctggaagggc tgctgccaga agcactggtt gcaggtattg ccgatgcgct ggaaggcaaa
     1381 catccggctg ttccggttga tgtggtgcat cgcgcgctgc gtgaaatcca cgagcgcgcc
     1441 gatgccgttc gtcgtcagcg tttccaggcg atggctgctg aaggtgtgaa atacctggaa
     1501 gaaaacgcca aaaaagaagg tgtgaatagc accgaatctg gcctgcaatt ccgcgtgatc
     1561 aaccagggtg aaggcgcaat tccggcacgt accgaccgcg ttcgtgttca ttacaccggt
     1621 aaactgatcg acggcaccgt gtttgacagc tccgttgctc gtggtgaacc cgctgaattt
     1681 ccggttaatg gcgtgatccc tggctggatt gaagcactga ctctgatgcc ggtaggttct
     1741 aaatgggaac tgactatccc gcaggaactg gcatatggcg agcgcggcgc aggcgcatcc
     1801 atccctccgt tcagcaccct ggtgtttgaa gtcgaactgc tggaaatcct ctaagcagcg
     1861 cattctgttc ccctcgaacc agaggggagc aggcattcag caataaaccc ttcagtttgc
     1921 caaacggcgc tattttgtgt tgcaaagacc ccgtaagcgt gtatttttgt gagctgtttc
     1981 gcgttatcac cgtgatatga cactcacttt aaacataaaa ttaacattag atctaaatct
     2041 tagtattcat cccgcgtatt gttacctaat atcgatgagt cccgatacag attcgtcgta
     2101 tcatagactg actaaaggcc gtagagcctg aacaacacag acaggtacag gaagaaaaaa
     2161 acatggtaga tcaggtaaaa gtcgttgccg atgatcaggc tccggctgaa cagtcgctac
     2221 ggcgcaatct cacaaaccga catattcagc ttattgccat tggtggtgcc attggtacgg
     2281 ggttgtttat ggggtctggc aaaacgatta gccttgccgg gccgtcgatc attttcgttt
     2341 atatgatcat tggttttatg ctctttttcg tgatgcgggc aatgggggaa ttgctgcttt
     2401 cgaatctgga atacaaatct tttagtgact tcgcttccga tttactcggg ccgtgggcag
     2461 gatatttcac cggctggact tactggttct gctgggttgt aaccggtatg gcagacgtgg
     2521 tggcgatcac ggcttatgct cagttctggt tccccgatct ctccgactgg gtcgcctcgc
     2581 tggcggtgat agtgctgctg ctgacgctta acctcgccac tgtgaaaatg ttcggtgaga
     2641 tggagttctg gtttgcgatg atcaaaatcg tcgccattgt gtcgctgatt gtcgtcggcc
     2701 tggtcatggt ggcgatgcac tttcagtcac cgactggtgt ggaagcgtca ttcgcgcatt
     2761 tgtggaatga cggcggctgg ttcccgaaag gtttaagtgg cttctttgcc ggattccaga
     2821 tagcggtttt cgctttcgtg gggattgagc tggtaggtac aacagctgcg gaaaccaaag
     2881 atccagagaa atcactgcca cgcgcgatta actccattcc gatccgtatc attatgttct
     2941 acgtcttcgc gctgattgtt attatgtccg tgacgccgtg gagttcggta gtcccggaga
     3001 aaagcccgtt tgttgaactg tttgtgttgg taggtttgcc tgcggctgcc agcgtgatca
     3061 actttgtggt gctgacctct gcggcgtctt ccgctaacag tggcgtcttc tctaccagcc
     3121 gtatgctgtt tggtctggcg caggaaggtg tggcaccgaa agcgttcgct aaactttcta
     3181 agcgcgcagt acccgcgaaa gggttgactt tctcgtgtat ctgtctgctc ggcggcgtgg
     3241 tgatgttgta tgtgaatcct agcgtgattg gcgcgttcac gatgattaca accgtttccg
     3301 cgattctgtt tatgttcgtc tggacgatta tcctttgctc gtaccttgtg tatcgcaaac
     3361 agcgtcctca tctacatgag aagtcgatct acaagatgcc gctcggcaag ctgatgtgct
     3421 gggtatgtat ggcgttcttt gtgttcgtgg tcgtgttgct gacactggaa gatgacactc
     3481 gccaggcgct gctggttacc ccgctgtggt ttatcgcgct ggggctgggc tggttgttta
     3541 ttggtaagaa gcgggctgct gaactgcgga aataaccgca ttatcatgct ggatggcgca
     3601 atgccatcca gcttttagat cactcacccg ccagcgccct tgggaacagt acattgtttt
     3661 ccaggctgat gtgatccatc aggtcatcaa tcagttcatt aatgccgtta tacatcgctt
     3721 tccaggtggt gcaggcttcc ggcggcggtg tgacgttatt ggtattgtgt ttgatcactt
     3781 ccagcagttc acccgcttca tcgtgctcgc tttccattac gctgattggc cccattgcct
     3841 ggctgcccat gccttgtttg atcatcggga agaggatctg ctcttctttc atcatgtggc
     3901 tggaaagctc ttcatgcagc atggtcaggt attttgtcag cccttttggc acgctcggtt
     3961 tgtcggcgtg aacgcgctcg actttagtcg cttgcagaat cagctccggc agttgctcgc
     4021 ggtgacgagc gtggtagcgc acgatgatat ggtcgatgat ttctgccagc ggggcgctac
     4081 gccagtcttt ctcaatcggt tgttcagcaa gctttgccag ctcagcttca atgacctcaa
     4141 catccagttc tttacgtgcc gccgcacgcg ccagcgtttg cttaccgccg cagcagtaat
     4201 ccatatcata tttacgaaac agagccgaag cgcgaggaat agagagcgcc agttcaccta
     4261 aaggttggtc gcgataagcc atagctgata cctcattctt aataagataa gatgtatttt
     4321 aaatgcatct ttaaggcaaa agctataacc cttacgtagc agaggcttat ttaactcact
     4381 gccagcgtgt caactttctg agacagattc agttaaaggt ttttctggtt ttgctctaac
     4441 cgcgatgacc acgccaatca ctaacagcgc gataccgctc agcgtcatta gcggcggcat
     4501 ttgctggcgg agtaaaaagg tgtacagcaa acctgccagc gtttcgaaaa caatcagcgg
     4561 cccgagaatc actgtcggta atcgctggct ggcgacgttc cagcagagtg cgccaaccca
     4621 tgagcaaagc acggctatcg caatcatcag actaataaac accagcggac gggggccaaa
     4681 gggtagggag aagtccggcg tttgcgtatt cagccagtaa caagcgacga gatagccgat
     4741 aagtgaaacc ggcagtgtga ccagcgcctg cgctgttgcc cacatcatcg gatgtttgtc
     4801 gggattttcc cgcagccagc gggcgttgcg cagggcatac cacgcccagc agaccacgga
     4861 aactaacgcc agcacgatgc cagaggtata acgtgcccag tcaaaattgg ggagtccgtg
     4921 gtttaactca gcaatattca cacacgccag gccgatgcca atacaaatca gtgccggggc
     4981 gagttttccc cacgcgagtt tgccgtcgcg ctggctataa agcagattgg cgaagacggg
     5041 aatcaccacc ggcagggtgc cgataatcat cgtggaaaca ggcgcaccag tacgttgaat
     5101 ggcactggca aggcagaagt aatagatgag gttgcccatc atagtgagca tcaaggcggt
     5161 aagccagtcg cgacgcgcca actgacgcag acgcacgcgt cccagccagg caatgggcag
     5221 cgcaattaac cctaacgcca gataacgccc catcgactgc aacatcgccg ggtattccgg
     5281 cacgatcaac ggcccgacaa aaataagccc ccacatcaac cctgctaaca gggcgtacag
     5341 cacgccgcta atcattactg gcatccattg atctgtcaga agaaagttca ggaagcagac
     5401 attattgctc ccggcatccc gtcattatca gagaggatgc gggaggatgc cactttaatt
     5461 aactagttat taacattaaa aagatggctt acgctttcgg ctaacgttgt cgttgggtgg
     5521 ccaatcaatt tgctaagtgt tttgctgtca tcaaacagac cgcctttcga tgcgccaacg
     5581 tcagaatccg ccagcatatc cgccagtccg tcgggcagtc cgacgctttt cagtgccgcg
     5641 gcgaaatcgg cttcgctcag attttgatag gtaacctgtt tgccgctctg tttggtcagc
     5701 tctgccgcta actgtgtcaa cgtccaggca ctatcgcccg ccagttcgta aaccttgcct
     5761 tcgtgaccgg cttcgctaat cacgcgtgcc gcagctgccg cataatctgc ccgcgttgct
     5821 gaggcgattt tgccatcgcc cgccgcaccg ataaatacgc cgtgttccag tgctgccggg
     5881 gcgctggcga ggtagttttc ggtgtaccag ccgttgcgca gtagggtgta aacgatgcca
     5941 gaatcagcca gcattttctc cgtctcgatg tgctcatcgg cgaggccgag cggagattta
     6001 tccgcgtgaa gcaggctggt ataagcgata aatttcacgc cagccgtctt tgcggcatta
     6061 ataacattac gatgctgcgg ggcacgttga cccacttcgc tggaagagat cagcagtagt
     6121 ttttccactc cctgaagtgc agatgtcagt gcggcttcat cgccgtagtc agcctgacgc
     6181 acggtaatgc cttgtgctgt cagtgcctgg gcttttgccg gattacgaac gatagccact
     6241 atttggctgg caggaaccgt tttcatcaag gattcaataa catactgacc aagttggcca
     6301 gtggcaccag taatagcgat catgggaagt ctccatcgtt ttgtcttgtg tttaaatacg
     6361 ctaacaccta aacttacttt tagtaagtac gcacaaaaag gtaagtatga aatgagtcag
     6421 gttagcctgt cgcaacaact gaaagagggt aatctctttg cggaacagtg cccgtcgcgc
     6481 gaggtgttga aacacgtcac cagccgttgg ggggtgttga ttctggtggc gctacgcgaa
     6541 ggtactcatc gctttagcga cctgcggcgc aaaatcggcg gggtgagcga aaagatgctt
     6601 gcgcagtcgt tacaggcgtt agaacaggat ggttttctta accgtatcgc gtatccggtg
     6661 gtgccgccgc atgttgaata cagcctcacg ccgctgggcg agcaggtgag cgaaaaggtt
     6721 gctgcactgg cggactggat tgagttgaat ttgcccgagg tgttggcggt gcgggatgaa
     6781 cgtgcggcat aactgccgga tgcgcggcct acggtgtatg acttgtaggg cggataagat
     6841 gcgccagcat cgcatccgcc aataaatgcc ggatgcgatt gatgatttat aggccgaaaa
     6901 agaagtgttt tacttactca aatccacctg ataaatcgca aacccgatat catcggtcgc
     6961 gactttattc atcggatact gccctttctc tttaataaac gctgcggctt tatctgacgg
     7021 ggaagtttcg aaacggatat ccagtttctt atcggcagct atcggtgcta aacgccagtt
     7081 gttatctgcc gccgggtgaa tttcccccgc acgtttcgac tcatcagcaa tccacgctgc
     7141 cagcaccgag cggttctcat ccggtgaagc aaaagcgata tggctgtcgc ccgtcccggc
     7201 aaatttgccg ccgtaagcgc gatagttatt ggtggcaacg aggaacatag cgttcggatc
     7261 aatcggcttg ccattaaagg tcaggttctt aatcctttcc gcattggcat taatcatctg
     7321 gcactcgccg tcataacggg cgggctgggt aacatcaatc tgataattca caccatcaat
     7381 aacatcaaag ttataagtgc ggaaaccatc ccagttgatg agtgactgtg gtttcgtgct
     7441 gttgggatca atctggttaa actgtccggc ggagcactcc agccactctt tcacctcttt
     7501 accgctggct ttcaccacaa tcagcgtatt gggatagaga taaagatcgg cggcattacg
     7561 gaaggtcaac tggccttttt ccacctccac atagcttgcc gggtcatttt tgcgaccacc
     7621 gactttaaac ggtgcggcag ctgaaagcac cggcagtttt gccagatccg gatcgccctg
     7681 aatgtaatgc tcgacatacg ctttttgcgc gttgttcact acttgcacgg tcggatcgtc
     7741 ctgcaccagc gccagatagc tatacatatt gtcggcagat ttaccgattg gcttgctgac
     7801 gaactggcgt gtggcatcgt gatcggcttt gagtgtttct accagcttgc tgtcttccgc
     7861 cgcgagggat tttttattgg cgatgtcgta aatcggccgt gcttccgctt tcgcctgcgt
     7921 cacctgccat ttaccgctgt cattactgag ttgtaagtcg accaccccaa gatgatcgcc
     7981 ccacatgccc ggcattaccg ccggaacacc attcagcgtg cctttggtga tatcagcccc
     8041 ttcgatatca gcaaaatctt tacccgggaa aacggcgtga gcatggccaa acataatggc
     8101 gttaacgccc ggaatttcac tgaggtaata aactgagttt tccgccatta ctttatacgg
     8161 atcggcagac agcccggaat gcgccagaac gacaacgaca tcggcacctt tctcgcgcat
     8221 ttcaggctcg tatttgcgca cggtttcggt aatatcattc accgtcactt ttccggataa
     8281 attagcttta tcccagccca tgatttgcgg cggcacgacg ccaatatagc caatcttcag
     8341 cgtctgtttt tttccgtctt tatcgaccac ttcggtgtct ttaattaaat acggtgtaaa
     8401 cattggctgt ttggttctgg cgtcaatgac gttggcattt acataaggga atttcgctcc
     8461 tgccagcgca tttttcaggt aatccagacc gtagttaaac tcgtggttgc caagcgttcc
     8521 gacggtatag tccagcgtat ttaatgcctt atagaccggg tgaatatcac ctgcttttaa
     8581 tcctttcgcc gacatgtaat cggccagcgg actcccctga atcaaatcgc cgttatcgac
     8641 cagtacgctg tttttcactt cattgcgggc attgttaatc aggcttgccg tacgtaccag
     8701 tccgaatttt tccgtggcgg tgtctttgta ataatcgaaa tccatcatgt tgctatgcag
     8761 atcagtggtt tccatgatac gcagatcgac cgtcgctgca ttcacactgg cggcaatcag
     8821 cgtggccagg agcgttgcgc taaacttaat catcagggac atccttttat catcgggaat
     8881 acgaaagaaa agggagaata aacgtcttac ttatagaaca gtgaagaatg ccacaatttt
     8941 acgctttgaa aatgatgaca ctatcacagt tggcgcattc attaatgata gggtataagt
     9001 aaaacaataa gttaacaccg ctcacagaga cgaggtggag aaatgttaga tcaagtatgc
     9061 cagcttgcac ggaatgcagg cgatgccatt atgcaggtct acgacggggc gaaaccgatg
     9121 gacgtcgtca gcaaagcgga caattctccg gtaacggcag cggatattgc cgctcacacc
     9181 gttatcatgg acggtttacg tacgctgaca ccggatattc cggtcctttc tgaagaagat
     9241 cctcccggtt gggaagtccg tcagcactgg cagcgttact ggctggtaga cccgctggat
     9301 ggtacgaaag agtttattaa acgtaatggc gaattcaccg ttaacattgc gctcattgac
     9361 catggcaaac cgattttagg tgtggtgtat gcgccggtaa tgaacgtaat gtacagcgcg
     9421 gcagaaggca aggcctggaa agaagagtgc ggcgtgcgca agcagattca ggtccgcgat
     9481 gcgcgcccgc cgctggtggt gatcagccgc tctcatgcgg atgcggagct gaaagagtat
     9541 ctgcaacagc ttggcgaaca tcagaccacg tccatcggct cttcgctgaa gttctgcctg
     9601 gtggcggaag gacaggcgca gctgtacccg cgtttcggac caacgaatat ttgggacacc
     9661 gccgctggac atgctgtggc tgcagctgcc ggagcgcacg ttcacgactg gcagggtaaa
     9721 ccgctggatt acactccgcg cgagtcgttc ctgaatccgg ggttcagagt gtctatttac
     9781 taaattcaga tggcagaaac agtgtatttc ctgattctgc catcct
//
LOCUS       DAZKIF010000151         9815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZKIF010000151 DAZKIF010000000
VERSION     DAZKIF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.152"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..162)
                     /locus_tag="ACHOTW_004027"
     CDS             complement(<1..162)
                     /locus_tag="ACHOTW_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846041.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLT"
     gene            240..1334
                     /gene="ddlA"
                     /locus_tag="ACHOTW_004028"
     CDS             240..1334
                     /gene="ddlA"
                     /locus_tag="ACHOTW_004028"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9846042.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1358..1570)
                     /gene="yaiZ"
                     /locus_tag="ACHOTW_004029"
     CDS             complement(1358..1570)
                     /gene="yaiZ"
                     /locus_tag="ACHOTW_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP9846043.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1830..2138
                     /gene="yaiY"
                     /locus_tag="ACHOTW_004030"
     CDS             1830..2138
                     /gene="yaiY"
                     /locus_tag="ACHOTW_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP9846044.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2197..3291)
                     /gene="yaiW"
                     /locus_tag="ACHOTW_004031"
     CDS             complement(2197..3291)
                     /gene="yaiW"
                     /locus_tag="ACHOTW_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP9846045.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3304..4524)
                     /gene="sbmA"
                     /locus_tag="ACHOTW_004032"
     CDS             complement(3304..4524)
                     /gene="sbmA"
                     /locus_tag="ACHOTW_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFP9846046.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4876..6033
                     /gene="ampH"
                     /locus_tag="ACHOTW_004033"
     CDS             4876..6033
                     /gene="ampH"
                     /locus_tag="ACHOTW_004033"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP9846047.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6034..6657)
                     /gene="iprA"
                     /locus_tag="ACHOTW_004034"
     CDS             complement(6034..6657)
                     /gene="iprA"
                     /locus_tag="ACHOTW_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP9846048.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6745..9687)
                     /gene="ehaB"
                     /locus_tag="ACHOTW_004035"
     CDS             complement(6745..9687)
                     /gene="ehaB"
                     /locus_tag="ACHOTW_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFP9846049.1"
                     /translation="MHSWKKKLIVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMITSECMTTDCADDRATGYVYDRLTLSVDNSTIDDNYEHYTYNGTYN
                     NAADTHVVDVYDMGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNS
                     SEVFNNTITVKDSTVTSGSWTDEGTTGWFGHTGNASNYSNTLTADDVAIAAIANPYAD
                     NAMQTTVTLDNSTLMGDVVFSSNFDENFFPQGANSYRDADGDVDTNGWDGTDRMDVTL
                     NNGSKWVGAAMSVHMVDEDGDGSYDGYAVGTEATATLLDIAANSLWPSSTVGVDNINT
                     QYDENGHIVGNEVYQSGLFNVTLNGGSEWDTTKSSLIDTLSINSGSQVNVADSRLISD
                     TVSLTGGSNLNIGEDGHVATNTLTIDNSTVKMSDDVSAGWGLEDAALYANTITVTNDG
                     LLDINVDQFDANPFQADTLNLTSTTDTNGNIHAGVFDIHSSDYVMDTDLVNDRTNDTT
                     KSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDN
                     ATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTD
                     YANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINY
                     DQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFA
                     NNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPY
                     GSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYV
                     YDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSA
                     NVGVKYTW"
BASE COUNT         2522 a         2435 c         2332 g         2526 t
ORIGIN      
        1 cgttaactta taattaagag aaaaaaactc accgcatggc catttaagcc aggaataaat
       61 tagcttcccg ttcatcattt ttgtgatctt gcgcacatct ttgagataac gcactttctt
      121 caccagtacg ttgcacgctt tgtcagcaga agattgcaac aatctttcag aaggccgcga
      181 atcccccgtt ttgtcgtggt aatgtatgca ctttgtgtta gacgggatag gtttttaaga
      241 tggaaaaact gcgggtagga atcgtttttg gtggtaaatc agcggaacat gaagtgtctc
      301 tgcaatcggc aaaaaacatt gtcgatgcca ttgataaaag tcgcttcgac gttgtgctgc
      361 tgggcattga taaacaaggg caatggcacg tcagcgatgc cagcaattat ctgctaaatg
      421 cagacgatcc tgcccatatt gcgttgcgcc cttcggcgac cagcctcgcc caggtgccag
      481 gtaaacatga gcatcagctt atcgacgcgc aaaacggtca gccgttgccg acggtggatg
      541 tcattttccc gattgtccac ggtacgctgg gcgaagatgg ttccttgcag ggaatgctgc
      601 gggtcgccaa tttaccgttt gtaggttctg atgttctggc ttcagcagcc tgtatggata
      661 aagatgtcac caaacgtctg ctacgcgatg ccgggctgaa cattgcgcca tttattaccc
      721 tgacgcgcgc taatcgtcac aacatcagtt ttgccgaagt ggagtctaaa ctggggttac
      781 cactgtttgt aaaaccggct aatcagggct cttctgttgg tgtcagcaaa gtaaccagtg
      841 aagaacaata cgcaattgcc gtcgatctgg cgttcgagtt cgatcataaa gtgatcgttg
      901 agcaagggat caaaggtcgt gagatcgaat gcgcagttct gggcaacgac aatccgcaag
      961 ccagcacctg tggcgagatc gtactcacca gcgatttcta tgcctacgac accaagtaca
     1021 ttgacgaaga tggcgcgaaa gtggtagttc cggcagccat tgcgccagaa atcaacgata
     1081 agatccgggc gattgccgtt caggcttatc agacgttggg atgcgcaggc atggcgcgtg
     1141 tagacgtgtt tttaacccca gagaacgaag tggtgatcaa cgagatcaac acactgcctg
     1201 gcttcaccaa catcagtatg tatccgaagc tgtggcaagc cagcggtctg ggttacaccg
     1261 atctgatcac acgtttgatt gagctggcgc tggagcgtca cgctgcggat aacgcattga
     1321 aaaccacaat gtaatatttt aacgcccgat aatattctta ttgggcgttt tcagtttctt
     1381 catcgcgacg acgaataatc aaccccgcca gccagaaact aatcacccac gtcaccagac
     1441 cgacggcata ggtctgccaa ccttttgctt caaatcccag taaccccacc acgccattaa
     1501 gaatgaatat aaggccaatg gcgaacgcat agtagtgcca gtcacggcgg atttttacag
     1561 gcagattcat ggctgctccg ttaacgaaaa acaacatcct tgcatattct gcaacaaccg
     1621 tctgtggtgt agacaacatt ttcgcaattg ttaataaatt taacttaaag ttgctatttt
     1681 gtgactcgca acgaagattc acgcttcggg aatattccca acatcaattt accatgattc
     1741 tgatattgac aatctaaatg acctgtcaga cttgccataa gccgctggta tcacaccaga
     1801 agagaagcag atgacttccg gaggttttta tggctgattt caccctgtca aaatcgctgt
     1861 ttagcggaaa atatcgcaat gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg
     1921 tgctgttttg cttttgggct ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg
     1981 tctatgagcg tttaatgcag gtccaggaaa caggtcgccc acgggtggaa attggtttag
     2041 gtgtcggcac cattttcggg ctgatcccgt ttttagtagg ctgcctcatt tttgcagtgg
     2101 tggcgctatg gctgcactgg cgacatcgcc gccagtaaga tttacgttgc cagatacggc
     2161 ctaaaacgcc ttatctggca tgacattcct gtttgttcac tgtttcatac aacgcgcccg
     2221 ccgttcgtct acgcgcttcg caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct
     2281 ttccagttga atgccaggca acatttcgcg ggggagtgat ttacccgttt tcgcttcggc
     2341 aagttgataa actttcttgt acagcgccgt ctcctcaaaa gagaagctgt cacctttctc
     2401 tagctgacga cgaatttcgc tgtcgctcat ccccagtttt gccgccagtt tgcgcgttgc
     2461 cagttcggtt ttcccgggtt ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag
     2521 cttcacgccg ctggccttac tgaccgcgtt ctgaaacgcg gcattacggc tggcgtacca
     2581 gccagcgtta aaatcagcaa aacggtatat cggtgcgcta taactggcgg gataattcag
     2641 taaatggtaa gtaccaaacc acaacccgcc gcggcgacta aagacttcct gacggactgt
     2701 accgtccatt ttccacggat accctttggt atgctgttcg gcaaaagcaa tgctgacctg
     2761 catcggccca ccggtgcgca ccggattaag cgaaccaaac aacgtctgcc ccattggcac
     2821 catgctgatc agatcgtcaa aaatcccgct caattgcttt tctgtacgca ccgaatctaa
     2881 ccgttcgcta taacttttgc cgtttggcga tttgattttt agcgcagtat gaaccagaaa
     2941 agcaggaatg tgcatccgtt cagcacgacg gtcaatctct tgccaggcga tcttgcttaa
     3001 gcccggaacg gccggatctg cctgataatt tgactcctgc tgcgccaccg ccagcaccga
     3061 gcagacgttt tccagcgtcg gtgccagccc ctgactttca aaggtggtgg cgagatcttt
     3121 cgcccaggca tcgcggtctt tcacgctcgc aggcatcttc tgccgcacca cgctagccac
     3181 atctatcgct ttttcgccct ttttcaacgg ctgtggcgcc tgactgctac accccgctaa
     3241 aaccagaacc gccaaaaatg aaagcgaaga cagatgattg actcgcgaca ttacttctcc
     3301 tttttagctc aaggtatggg ttacttcctg aattttgtca ccatccagct catgttcaaa
     3361 gctgcgcaga cgtttgtaga tagacatcaa ctcaaccagt gtggtccatg agttaatcag
     3421 gtactggaaa gcaccgcgaa cctgaccaaa aacgttggta atctgcgtca tcaggccgag
     3481 cgtaatcgta ccggcaacaa ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac
     3541 ctgcaaatag agaatgcgag cgatgttgaa atacatatag tgaaaataga ggcggaaata
     3601 gtttttccgt acggcgctaa acagctcgcg taccgttggc ggcgtcgcgc gcgtggcatc
     3661 gtcttcacca taaaccagct ctttacggta ggcagcctct acacgctggt ttttaaactc
     3721 cagccccggc agtttgatcc ctaccactgc cagcaatccg gtccccatca gcgaccagac
     3781 gatagcggca atcaccagac catacggaat gtgcccgata atcggcagct ccggcacatg
     3841 cgcggagagc gttaccaaca ccggcaggaa ggcgatcaac gtcatgatgg cgttgataaa
     3901 actgacaccc atattctcca gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg
     3961 tgcggcgcct tcgatatgac gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc
     4021 cgtacgccag cggaacacgt agtgactgac aaagaagtta ttcagcacac tgatcactac
     4081 cgcgatcagc gcaatcccca gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac
     4141 tttatgcggc gaacttagcg cggtttgaat cagatcatag aacggtgcat accaggcgtt
     4201 gacggcaacc ccaacttcca ccaaaaacca ggtgacgaag atgatcagtg cagtaccgag
     4261 tattgaccag tattgccaac ggtgcgggct gtagataaac cagaacaatg caaaaagtcc
     4321 tacgcaaaca atgtagtaag cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc
     4381 gctaatcggg atctgcccgg aagcgccggt gatacgcgcc acccagtccc ccccaccggc
     4441 ttgccagaag ataacggcaa tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg
     4501 ctttgggaaa aaagacttaa acatatgcac tcctgctaac ttcttatcgt tttaacaact
     4561 gctgtgtatt aaccgcatga ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga
     4621 ctcatagcaa ggcgcttagt tcgccagcct ggccataaga attgtttcga cggatttcac
     4681 aaaccaaaga ttgtttacat aagcggatta acacgcgcag gatcacaaat gcaggcattg
     4741 gtgagcagga aaaagatcgt gtttacgcgc cgttacgttt ttaacactat tattgcacta
     4801 attcgccctt tgcaatccat caatgagtag tataaatacg ctcagttacc ttcattcaat
     4861 ttatggacac caccgttgaa acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca
     4921 ttgacctccg tccatgcggc gcagccgatc actgaaccgg agtttgcctc tgatattgtc
     4981 gatcgttatg ccgatcatat tttttacggc agcggtgcca cggggatggc gctggttgtt
     5041 atcgacggta accagcgcgt ctttcgcagt tatggcgaaa cgcgacctgg taataacgtt
     5101 cgcccgcagc tggattcggt cgtgcgtatt gcttccctca ccaagctaat gaccagtgag
     5161 atgctggtga aattgctcga ccaggggacc gtgaagttaa acgatccgct aagtaaatat
     5221 gctccgccag gcgcacgcgt gccaacctac aatgggacgc ccattacgct ggtcaatctg
     5281 gcaacccata ccagcgccct gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg
     5341 tttgtctggc caacgcgcga gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc
     5401 gcgccaggca gtcaggcggc gtactctaac cttgcgttcg acctgctggc agatgcactg
     5461 gcgaatgcgt ccggcaagcc ttatacccag ttgtttgaag agcagattac ccgtccattg
     5521 gggatgaaag acaccaccta cactccctca ccggatcagt gccgccgttt aatggttgct
     5581 gagcgcggtg ccagtccgtg caataacacg ctggcggcaa ttggcagcgg cggcgtctat
     5641 tccacgcctg gcgatatgat gcgctggatg cagcagtatt tgtcatctga tttttatcag
     5701 cgcagtaacc aggcagatcg catgcagacg ttgatttatc agcgcgcgca gtttacgaaa
     5761 gtgattggca tggatgtccc cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg
     5821 gccccgaaag agggtcgtcc ggggattatt cagaagacag gtggtggtgg cggtttcatt
     5881 acctatatgg cgatgatccc gcagaagaat attggcgcgt ttgtggtggt cactcgttcg
     5941 ccgttgacgc gctttaaaaa tatgagcgat ggcatcaatg atctggtgac cgagctaagc
     6001 gggaataaac cgttggttat ccccgcatcc tgattaatac tctgaaggca aacggttgat
     6061 agcgaccagt ttgcctttat tcatttcgat atagccgcct ttacgcaaag cagcgagaac
     6121 ttctgcgacg acagaacgcg atatgcgtgt acgttgatgg atataattca tcacaccaat
     6181 acgtgatcgc aattcttcat tccagtcaat cattgataat aatgttgcgc ggatttgttc
     6241 gtaggaatta tgcccaatga gctgcacgtc gcgtaattcc agaattctat tttgccaggc
     6301 taaccagtaa aaagcgtctc gccagagttg attttgttca ataagcgtaa tggtttgttt
     6361 ggctggtaga tgatatcccg tacaatttcc ttctgatatt aatttgtatg gtatatcgtt
     6421 tttcattaaa ccatcagcca gccccataat ataaggagcc tgggtaatac cgataagtac
     6481 gttttcttct ctacgcagag agataactcc ctctaaaata acgaaagtat cttcgttatt
     6541 gacatcactg gaaaatataa cttgcttttc attattaaac tcgaagcgcg taccgtatct
     6601 ggacaaacat ttatcgagct taccaaattc ctgaagaggt ttaactacag ataacatttg
     6661 cgcgtccttt gcagtaatgc ccgtcaaatc cttgacgggc attatttaga ttaaattacc
     6721 agtatttctt cggagtgaag aatattacca ggtatattta acacccacgt tcgcggacca
     6781 gtcttgatct acgtcaccac caccgaggta gttagcatcg gtataggcgc tgaagttctt
     6841 ggtgaagctg aactgagtac ccagaccaac acgtaccgca gacccttcag taccgttatc
     6901 gatggaatca ccgttcacat cgttatcgtt gttagagtcg tcgtagacgt aagccagttt
     6961 gaagtacgga gtcagagcct gatcttcgct gtaggtgaag gtataacctg catctacacc
     7021 cagttcataa cgcatgctgt cgtaagactg accgtcaact ttcatgtcgt tgctcagctg
     7081 gtagtcatca ccagactgga acagaccaga aatgctgccg taaggagtca cgtaaccagc
     7141 atcacccagt ttgaagtcgt aaccggcttt caaaccgaag ccccaagcgt cggagttggt
     7201 gctaccgtca acgtaagtac cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga
     7261 gtagctcaag ctaccatcaa caaagacgtt gttcgcgaag tgagcagaag agtagatgta
     7321 ggcagtctgg ctgtcttgat ccacctgacc agaacggtca ttcatgtcac ctttagcgaa
     7381 gcctgcagcc gcaccgacga tccacttagc gttgttaccg tcaattttgg tatcaacacc
     7441 gaccatgatg ccgttaacat cctgatcata gttgatggtg ccgttgtcgc cgttgaagtt
     7501 accaccgaag tagcttaccc atgcgccgcc gttatcagcc aggccatgac gagagttggt
     7561 caaacgagta ccaacggtgt cttgttccag gttccagata ttggtgttcg cagatgggat
     7621 gctcagcgcc atgttagcgt agtcggtcaa ctccatctgt tgcagaacaa cggtgttacc
     7681 gcgctgttca gcctgatagg tgtatgcacc caggtcagct ttgttagcag cagagaaggt
     7741 cgcgttgctg tttttgtcgt tgacgtagat cagctcgttg ccgttgtagt cagcgataga
     7801 accagcacca gtagcgttgt cgatacgaac tttgtagtta ccggtcgcgg ctgcaacatg
     7861 gtcaccgttg ttatcaactt cgttctgacc agcttcgata gaagcagtgt tgtcgttatc
     7921 gccgttaccg ttaatagtca ggtgaccatc agagttcatt gcgattaagc cataaccgta
     7981 gtttgacttg gtagtatcgt tggtacgatc gttgaccaga tcggtatcca ttacgtaatc
     8041 actgctatgg atatcgaata caccagcgtg aatgttgccg ttagtatcag tggtactggt
     8101 cagattcagg gtatcggcct ggaacgggtt agcatcgaac tgatcaacgt taatatccaa
     8161 cagaccgtcg ttagttacgg tgatggtatt tgcgtacagt gcagcatctt ctaaacccca
     8221 gcccgcagaa acatcatcag acattttaac ggtactattg tcgatggtca gggtattagt
     8281 cgctacatga ccgtcttcac cgatgttcag gttagaaccg ccagtcagag agacagtgtc
     8341 agagatcaga cgagagtctg caacattaac ttgggaaccg ctgttaatac ttaaagtatc
     8401 aatcagagaa gattttgttg tatcccactc tgaaccaccg ttcaaagtca cattaaacaa
     8461 accgctctgg taaacttcgt ttcctacgat atggccattt tcgtcatatt gagtattgat
     8521 gttatcaaca ccgacagttg atgaaggcca caggctgtta gctgcaatat cgagcagagt
     8581 tgcagttgct tcagtaccaa cagcatatcc gtcgtaagaa ccatcaccat cttcatcaac
     8641 catatgaaca gacattgcag cgccaaccca cttgctgccg ttgttcagag tcacatccat
     8701 acggtctgtg ccatcccaac cgttggtatc tacatcacca tcagcatcgc gatagctgtt
     8761 agcaccttgc gggaagaagt tttcatcgaa attactggag aaaacaacat cacccatcag
     8821 tgttgagttg tctaaagtta ctgtagtctg catcgcatta tcagcatacg gatttgcgat
     8881 tgcggcaatt gcaacatcgt ctgcagtcag cgtgttgcta tagttgctgg cattaccagt
     8941 atggccaaac caaccagtag taccttcatc agtccatgaa ccagaggtca cagtagaatc
     9001 tttaacagta atagtgttat taaacacttc gctgctgttt acgccagtgc tgactgtgtt
     9061 gtcgtcaata tcttcccact cataaccctg agtcagcgta atacctgcta catgagagtt
     9121 attagtgatg gacagatcaa cttcctgatc cagtgtaata gcagtaccca tatcgtaaac
     9181 atctacaaca tgagtgtcag cggcattatt ataggtaccg ttgtaagtat aatgctcgta
     9241 gttgtcatcg atcgttgaat tatcaacgct cagtgtcaga cgatcataaa cataaccagt
     9301 agcacggtca tcagcacaat cagtagtcat gcactcagag gtaatcatac cgtgaatagt
     9361 gctgtttttg attgtcagac tattggcgtt ggtattggta gaaataccgt cgtccagata
     9421 gtaggtagaa atcacgccgt taacggtagc gttattaatg accgggtaga tatcgccgtt
     9481 gtaatagcta tcagcggcat agttgttcca gcctacataa ccatcatagt aaacgtcatc
     9541 agcataggtg gcgtcgttgt agtgatggaa agtattgtaa gtttgaccag aaatatcgtt
     9601 ggtcgctgca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta attgtgatac
     9661 tataagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt aataagttga
     9721 tccatcaatt atcaatgcat agaaaacatc ttatgtttcg cgttgacgat tatgcggcgt
     9781 tatagaggaa tggttcaatt attctttccc ctaag
//
LOCUS       DAZKIF010000152         9690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZKIF010000152 DAZKIF010000000
VERSION     DAZKIF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.153"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..773
                     /locus_tag="ACHOTW_004036"
     CDS             <1..773
                     /locus_tag="ACHOTW_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9846050.1"
                     /translation="GWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLIN
                     QLASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGF
                     TASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIV
                     ALCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLF
                     ASDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            799..1593
                     /gene="yfaU"
                     /locus_tag="ACHOTW_004037"
     CDS             799..1593
                     /gene="yfaU"
                     /locus_tag="ACHOTW_004037"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFP9846051.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ACHOTW_004038"
     CDS             complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ACHOTW_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP9846052.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ACHOTW_004039"
     CDS             complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ACHOTW_004039"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP9846053.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ACHOTW_004040"
     CDS             complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ACHOTW_004040"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP9846054.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ACHOTW_004041"
     CDS             complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ACHOTW_004041"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP9846055.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7090..8448
                     /gene="glpT"
                     /locus_tag="ACHOTW_004042"
     CDS             7090..8448
                     /gene="glpT"
                     /locus_tag="ACHOTW_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP9846056.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            8453..9529
                     /gene="glpQ"
                     /locus_tag="ACHOTW_004043"
     CDS             8453..9529
                     /gene="glpQ"
                     /locus_tag="ACHOTW_004043"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9846057.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         2209 a         2401 c         2682 g         2398 t
ORIGIN      
        1 ccggctggtt ctggatgttt gtgattgaag gattgttggc agtcggcgct ggggtattca
       61 cattcttttg gcttgatgac acaccggagc aggcacgttt tctgagtaaa ctagaaaaaa
      121 cgttgcttat caatcaactg gcaagtgaag aacaacagaa agtgacttct cggctgagcg
      181 atgcgctgcg taatgggcga gtctggcaac tggcgattat ctacctgacc attcaggtgg
      241 cggtttacgg attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag
      301 tgggctttac agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct
      361 ggcttattcc gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat
      421 tactggcggc gggcattggt attggtctgt ccgggctgct ttctccagta ctggcgatcg
      481 tagcgctgtg tgttgcagcc atcgggttta ttgccgtgca gccggtgttc tggacgatgc
      541 cgacacaact gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgcttg
      601 gtgcagtggg cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttatttgcca
      661 gcgatgcggc gggattactg acgctggcag cggtggcggt catcggttcg ctgattattt
      721 tcactctgcg tgtaaatcgc accgttgcgc agaccgacgt ggcacatcat taaataggtt
      781 aaggaacacg atgaacgcat tattaaccat ccctttaaag aacgtttacg caagggcgaa
      841 gtgcaaattg gtctgtggtt aagctcaacg actgcctata tggcagaaat tgccgctact
      901 tccggttatg actggttgct gattgacggg gagcatgcgc caaacaccat tcaggatctt
      961 tatcaccagc tacaggcggt agccccctat gccagccaac ccgtgatccg tccggtggaa
     1021 ggcagtaaac cgctgattaa acaagtcctg gatattggcg cgcaaactct actgatcccg
     1081 atggtcgata ctgccgatca ggcacgtcag gtggtgtctg ccacgcgcta tcctccctac
     1141 ggtgaacgtg gtgttggggc cagtgtagca cgggctgcgc gctggggacg cattgagaat
     1201 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     1261 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacct
     1321 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     1381 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     1441 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     1501 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     1561 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     1621 gggactgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     1681 cgagcaccag gtcacgatca aagccctgtt ccagcatgcg caaggcaatt ttaatagctt
     1741 gttcatgcat gccttcctgc aaaccttcta atttaccttc ctgccaacca atttgatgtc
     1801 cttcctgccg tagccgttca gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt
     1861 cggcaatctc ctgaataaaa cgggtgaaac gggaggtatc gccgcattgc agcagataat
     1921 taaacagtgt ttgtagctgg ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc
     1981 tgtcgaccat accgattaaa tcgcggtcgc gaatatgctt ttgaatcagt tcaagcagag
     2041 ctatacgccg atgttgcatg atctcatcgt caggtacgat ggtaatatcc accaacggaa
     2101 aagcttcggt gtacaactgc cgggcaagtt gcggatcgtc aaactcatcc agccagttga
     2161 gtgagtacgg gtagggcgag gtttcgccat gataaaacag caacggcacc accagcggga
     2221 ctctgtcata gcctttgtcc aggtgacgct gcatggcggc agtggcatag cgcattagcc
     2281 gaaaagccat attcttttct gcagagcttt gatgttcaat cacgcagtag atatagccgt
     2341 caccgtcgct ggtttccacg gaccacaaaa catccgagta gtaagcgcgt aaactttttt
     2401 caataaaact agtgggttcc aggcgtaagg tttgcaggtt gcaaagcttg cgcagtggtt
     2461 ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg tgtgaacata aaggttttaa
     2521 atactgcatc atgcggggag gaggttgttg attcggtcat ggttgtccgt cacctgatat
     2581 ttatgatgac gcgacattac ggttatccca ggcctttgac atctgccagt ttcagccttt
     2641 gcgagacgtt ttcagaggaa ttttcactgc gttgcaacgc atacattggg cacggaatcg
     2701 tgtacgcaaa aatgcccggt aaccacatgc ttaccgggca tcagaaagga gtttaagcca
     2761 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag acttgtggac atctcaatct
     2821 gccatttaca ggtttcgcag tcggtgacca ccagatctgc gccgctttct tctatctggc
     2881 ggaacagtgg tgcgccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     2941 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggatgttac
     3001 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     3061 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     3121 acagccagcg ggtcgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     3181 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     3241 tgccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     3301 ctttatcggt aaagccgttg gcgatcagcg gtacgccgca gcatttttct ttgctgagca
     3361 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     3421 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatac tgcgcttgct
     3481 gagccgccac gctgcgatac cagcgacgaa acgtgccgaa ggagtatttc ggtagcgtgc
     3541 ggcgatgatc gatttttaac gccgcatcaa gcaactgccg caccggtttc agcgaggtag
     3601 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatggctta
     3661 aaacaaaatt gcgcagcgac gggcgcgtgg tgtcatattt cgcccgtgcg cgctggatga
     3721 tatcgccaat cttcacatcg gacgggcagg cgacttcgca acgtttgcag ttgatgcaat
     3781 atttcagtgc ctcgtcatac agtgcgccat cttttaaacg cagacgctcg ccatccggcc
     3841 cggcttgttt tggccctgga taaccgggat tcacccggct cactgggcag gcggtggtgc
     3901 agacggtgca cttaatgcag ttttcgaagc tggtgtcatt cattgttggc ctcctgcgcg
     3961 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     4021 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gtaaacaggt tttcgatggt
     4081 ttgccctgcc tgcgacgggc gtagcgtctg atcagtggtt acaccgaact gctgccacgg
     4141 ttgcggcgca aaaaaatctc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     4201 aaggccgaga atcggctctc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     4261 actgccgctg gcgagaaccg cgaaacgtgg acgcagcggg atatcggcgt gattgcgggt
     4321 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     4381 ccacacgcca ccctgacgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     4441 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     4501 acgccacagt ttgtcatcag ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     4561 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     4621 acgggcgata ttcaccgcgc ggaactcggt ggcgttattg cgcaggacat ccagttccgg
     4681 cagctctatt tctgcggttt caacggcaag gtcgagttca cgcaacgaag ctgccgcaag
     4741 gtgcgcctga aaatccatca gtccgctaat tcccactaca catattttct tcgcgggcag
     4801 cggccagacg gggacttctg gcgaacttag ccaggtagag cgcagagtgc cgagcggcgt
     4861 aatccgctgg tgaggcagtt ctacgctgcc ctgcaattgc gcaccgcttt cagcgattaa
     4921 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggc ccgagaagag agtaaggatg
     4981 tgcaggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca cagcttgacc
     5041 atctggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     5101 acgcgtgaca atggcacagc gcaggccgtg tttttgcagc tgcaggccac agagtaatcc
     5161 ggcgaggccg ccgcccataa tgatagtatc aaagcgcatc tttctgctcc ttctccagac
     5221 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgca tctccccagg
     5281 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     5341 gcgcggacgt cgtgacgtta aaacgttgca gcagtccagc agcgcggcag gcgcagagtt
     5401 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     5461 cgtttaaatt ttctaccgcg tactgcactt cacccgcagt taccgcttcg cactcacata
     5521 ccaggctacg gtgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     5581 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     5641 cgggttcctg tgaacccgga agtgccagat cggcggtcgt acaggggcgc gtgttgccca
     5701 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     5761 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     5821 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     5881 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     5941 gcagcagaat atcaacctct tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa
     6001 tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     6061 cggaaggttt acggcagcgg ttgatcacat gctgattaat gcggtgatcc atgatcagca
     6121 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     6181 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     6241 gatggttacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     6301 catgagcggt aaggataaca gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     6361 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     6421 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     6481 cttcgcaggc gcgaataaaa gtggcctgga aggagaggtc atcttccggc agggtgataa
     6541 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     6601 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     6661 ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     6721 gcagcccgcg cagggcacag tcgcgggcaa ttccggctcc cgttgcgccg ccgccaatga
     6781 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     6841 tttaccattt agccatagta aaaacatgag ttgtttgatt tcgcgcatat tcgctcataa
     6901 ttcgaaagtg agacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
     6961 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     7021 aaacggcagg ttctctcact gaatcaggct gttaatcata aataagacca cgggccacgg
     7081 aggctttcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgccgcg
     7141 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     7201 tatgctgctt actatttggt tcgtaagaac tttgcgctcg ctatgcctta cctggtggag
     7261 cagggattct cacgcggtga tttaggtttt gcgctttctg ggatctcgat cgcctatgga
     7321 ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc
     7381 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     7441 tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg gttggttcca ggggatgggg
     7501 tggccgccat gcggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     7561 gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta ttccgccgct gctgttcctg
     7621 ctggggatgg cctggttcaa tgactggcac gcggcgctct atatgcccgc tttctgcgcc
     7681 attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ctgtggcttg
     7741 ccgccgatcg aagagtacaa aaatgattat ccggacgact ataacgaaaa agcggaacag
     7801 gagctgaccg cgaagcaaat cttcatgcag tacgtgctgc cgaacaaact gttgtggtat
     7861 atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg
     7921 acttatctga aagaggttaa gcatttcgcg ctggataaat cctcctgggc ctacttcttc
     7981 tatgagtatg cgggtattcc gggcaccctg ctgtgcggct ggatgtcgga taaagtcttc
     8041 cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc
     8101 gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc
     8161 ggcttcctga tctacggccc tgtgatgctg atcggtctgc atgcgctgga actggcaccg
     8221 aaaaaagcgg caggaacggc agcgggcttt accggtctgt ttggttacct gggcggttcg
     8281 gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt
     8341 atggtaatga ttggcggcag cattctagcg gttatcttgt tgattgttgt gatgattggc
     8401 gaaaaacgtc gccatcagga attactgcaa aaacgcaacg gaggctaatg gcatgaaatt
     8461 gacgctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc
     8521 aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt
     8581 gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga
     8641 acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc attacctcga
     8701 tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta
     8761 cgcgatagat ttcacgctgg atgaaattaa gtcgttgaaa tttaccgaag gtttcgatat
     8821 tgaaaacggt aaaaaagtgc agacttatcc agggcgtttc ccaatgggta agtccgactt
     8881 ccgggtgcac acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg
     8941 gaaaaatatc ggtatctatc cagaaatcaa agcgccgtgg ttccatcatc aggaagggaa
     9001 ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaagacga
     9061 taaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga
     9121 acccaaaatg ggcatggatc tcaatctggt acagctgatt gcctataccg actggaatga
     9181 aacgcagcag aaacagccgg acggaagctg ggttaattac aactacgact ggatgtttaa
     9241 gccgggtgcc atgaaacagg tggcggaata tgcagatggt attggtccgg attaccatat
     9301 gttgattgag gagacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc
     9361 tcagcagaac aagctggtag tgcatcctta taccgtgcgg tcagataaac tgcctgaata
     9421 cacaactgat gtgaatcagt tatatgatgc tctgtataac aaagcgggtg taaatgggtt
     9481 gtttactgat ttccctgata aagcggttaa attccttaat aaagagtaat gctgctactg
     9541 caggatggcg ttcatgcgcc atcctctgct aaagcgaaat aatgctacta ccggttattc
     9601 ttttcattaa ccggtgcgtc ctgtaaaaaa gatgccaaca taattttcgt aaaactcatt
     9661 cgttaaaaaa gctaacactg ggcattgtta
//
LOCUS       DAZKIF010000153         9546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZKIF010000153 DAZKIF010000000
VERSION     DAZKIF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.154"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            103..813
                     /locus_tag="ACHOTW_004044"
     CDS             103..813
                     /locus_tag="ACHOTW_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9846058.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            855..3506
                     /locus_tag="ACHOTW_004045"
     CDS             855..3506
                     /locus_tag="ACHOTW_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9846059.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGATFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSTLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            3520..4050
                     /locus_tag="ACHOTW_004046"
     CDS             3520..4050
                     /locus_tag="ACHOTW_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9846060.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4063..4566
                     /locus_tag="ACHOTW_004047"
     CDS             4063..4566
                     /locus_tag="ACHOTW_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9846061.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4626..5540
                     /locus_tag="ACHOTW_004048"
     CDS             4626..5540
                     /locus_tag="ACHOTW_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9846062.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            5874..8153
                     /gene="ydeP"
                     /locus_tag="ACHOTW_004049"
     CDS             5874..8153
                     /gene="ydeP"
                     /locus_tag="ACHOTW_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP9846063.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            8401..8598
                     /gene="safA"
                     /locus_tag="ACHOTW_004050"
     CDS             8401..8598
                     /gene="safA"
                     /locus_tag="ACHOTW_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFP9846064.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVILLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIVNSLQD"
     gene            8672..9433
                     /gene="ydeO"
                     /locus_tag="ACHOTW_004051"
     CDS             8672..9433
                     /gene="ydeO"
                     /locus_tag="ACHOTW_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP9846065.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT         2716 a         2087 c         2146 g         2597 t
ORIGIN      
        1 gtctggaaac ccggtgactc gccaatgtat tagcaacatt taccgattgt ctgatgtaac
       61 ggttttcccg cttatcctct taacacatgg agaatgcatc agatgcaaac tacgcgtacc
      121 ccctactcta tttcctttat ggcgacagtg ttacttttat tgctctttgc ttgccatagc
      181 accgttgcca acgcagctgt cgcactgggt gcgacgcggg taatttatcc ggcaaaccag
      241 aaacaggttt tattgcctgt cacgaataat gatcctgcca gtgtctatct cattcagtca
      301 tggatagaaa atgctggtga ccaaaaagac acccagtttg tcattacccc acctcttttt
      361 tccatgcaag gtaaaaaaga gaatacgctg cggataatca acgccactaa tcatcagtta
      421 cccggcgacc gtgaaagcct gttctgggtt aatgttaaag ctattcctgc tatggaaaaa
      481 gaccagaaga atgagaatac attacagctg gcaattatta gccgcataaa aatgttttat
      541 cggcccacga acctggcaat ggcacctgaa gaagcgcctg caatgcttcg gtttcgtcgc
      601 agcggtagca atctcactct tattaatcca acgccctact tcattaccgt aacaaatatg
      661 aaagcaggaa atagcaatct cccaaacacc atggtgccgc caaaaggtga agtgtcggtg
      721 gatatcccgc atgctgccac cggggatatc agttttcaga cgatcaatga ttacggcgca
      781 ttaactccgc gtattaaggc aaccatgcag taagccagtg acctaattaa taataaactc
      841 aatcggaatt aataatgaca gcctttcgtg ccgcatttaa ggcatatcgt atgcatcagg
      901 tgttactact gcctcgcttt gcacggctta ccattgccct tagcttagca actgcggtat
      961 ttcccgttga cgcagagtat tattttaatc cacggttttt aagcaatgat cttgctgaat
     1021 ctgtcgattt atccgccttt actaagggac gcgaggcacc acctggtaca tatcgggtcg
     1081 acatttattt gaacgacgaa tttatgacta gtcgggatat tacttttatt acagatgata
     1141 ataacgcaga tctcatccca tgcctgagta cagatcttct tgtcagtctt ggcataaaaa
     1201 aatctgcgtt attagacaat aaggaacatt cagcagaaaa acatgtgccg gacaacagcg
     1261 catgcacacc acttcaggat cgtttggtcg acgcatcaac tgagtttgat gttggtcagc
     1321 aacatctgtc actctccgtt ccgcaaattt atgttggcag aatggctcgc ggctatgttt
     1381 ctcctgatct gtgggaagaa ggaataaatg ctgggcttct aaactatagt tttaatggta
     1441 attctattaa taatcgtagt aaccataatg caggaaaatc caactatgca tatttgaatt
     1501 tacagagtgg catcaacatt ggtagttggc gactacgcga taactcaacg tggagttata
     1561 acagtgggag tagcaattca tctgacagca ataaatggca gcatatcaat acgtcggctg
     1621 aacgtgacat tattccctta cgctcacggt taacggtagg tgatagttat accgatggcg
     1681 atatttttga tagtgtgaac tttcgtggcc tcaaaataaa ttcaacagaa gcgatgttgc
     1741 ccgatagcca acatggtttc gctccggtga ttcatggtat tgcccgtggc accgcacaag
     1801 tgagtgtaaa acagaacgga tacgatgttt atcagactac tgttccaccc ggccctttta
     1861 ctattgatga tatcaactct gcggccaatg gtggtgatct gcaagtaacc ataaaagagg
     1921 cagacggcag tattcagaca ttatatgttc cttattcgtc tgttccggtt ctccaacgtg
     1981 ctggatatac gcgttatgcg cttgccatgg gggaatatcg tagtggaaat aacctgcaaa
     2041 gctcccccag gttcatacaa ggtagcttga tgcatggact ggaaggaaac tggacacctt
     2101 atggcggaat gcaaattgca gaagattatc aggccttcaa ccttggtatt ggtaaagatt
     2161 taggactttt tggtgccttt tctttcgata tcacgcaggc caatacgaca cttgcagatg
     2221 gcacccgtca cagcgggcaa tcggttaaat ccgtctacag caaatccttc taccagacag
     2281 gaaccaatat ccaggtcgca ggatatcgct attctacgca aggtttttat aacttatccg
     2341 acagtgccta cagtcgaatg agtggttaca ccgtcaagcc tcctaccgga gacagcaatg
     2401 agcagacaca atttattgat tattttaatc tgttctacag taagcgtggt caggaacaaa
     2461 taagcatctc tcagcagctt ggaaattacg gtgcgacatt tttcagtgcc agtcgccaaa
     2521 gttactggaa cacgtcacgc agcgaccagc aaatatcatt tggattaaat gtgccgtttg
     2581 gtgatattac gacttcgctg aattacagct attccaataa tatatggcaa aacgatcggg
     2641 atcatttact cgcttttacg cttaatgttc ccttcagtca ttggatgcgt acagacagtc
     2701 agtcggcatt tcgtaattca aacgccagtt acagtatgtc aaacgatttg aaaggcggca
     2761 tgaccaatct atcgggggtt tatggcactc tgctgccgga taataacctg aattatagcg
     2821 ttcaggtcgg taacacccac ggaggtaata catcgtctgg caccagtggt tacagtactc
     2881 ttaattatcg tggagcttac ggcaatacta atgtcggtta cagtcggagt ggtgacagca
     2941 gccagattta ttacggaatg agtggtggga ttattgctca tgctgatggc atcacctttg
     3001 gacagccgct gggcgacaca atggttctgg ttaaggctcc tggcgctgat aatgtcaaaa
     3061 tagagaacca gaccggaatt cataccgact ggcgtggcta tgccatatta ccatttgcga
     3121 cagaatatag agaaaatcgt gtcgctctta acgcgaattc ccttgcagat aatgttgaac
     3181 tggatgaaac cgtggtcact gtcatcccaa ctcacggtgc tattgccaga gcaacattta
     3241 atgcacaaat cggcgggaaa gtattaatga cgttgaagta cggtaataaa agcgttccat
     3301 tcggtgcaat tgtcactcac ggagagaata aaaatggcag cattgtcgcg gaaaacggtc
     3361 aggtttatct gactggactt ccacagtcag ggaaattaca ggtttcatgg ggcaatgata
     3421 aaaactcaaa ctgtattgtc gattacaagc ttcctgaagt ctctcctgga accttgctga
     3481 accagcagac agcaatctgt cgctaaggaa caatagatta tgaaatacaa taacattatt
     3541 ttcctcggtt tatgtctggg gttaaccacc tattctgctt tatccgcaga tagcgttatt
     3601 aaaattagcg ggcgcgtcct cgattatggc tgcacagtct catcggattc gcttaatttt
     3661 accgtagatc tccaaaaaaa cagtgccaga caatttccaa cgaccggtag cacaagtcca
     3721 gccgtccctt ttcagattac gttaagtgaa tgcagcaaag ggacaacggg ggttcgggtt
     3781 gcatttaacg gtattgagga cgcagaaaat aatactctgt tgaaactgga tgagggaagc
     3841 aatacggcct ccggtttagg tatagaaata ctggacggaa atatgcgtcc ggtgaaactg
     3901 aatgaccttc atgccgggat gcagtggatc ccactggtac cagaacagaa caatattttg
     3961 ccttactccg ctcgtctgaa gtcaactcag aagtccgtca atccgggact ggtgagggct
     4021 tcggcaacct ttacccttga atttcaataa tcagagtcag atatgaaaag acttcacaag
     4081 aggttcctct tagctacgtt ttgcgcgtta ttaacagcaa ctctccaggc cgccgatgtc
     4141 actatcactg ttaatggtcg ggtagtcgct aaaccctgca ctattcaaac caaagaagct
     4201 aacgttaatc tcggggatct ttatacgcgc aatctgcaac aacctggttc tgcatctggc
     4261 tggcacaata ttactttgtc attaaccgat tgtccggctg aaacaagtgc agtgacggca
     4321 atcgtgacag gttcaactga caatacgggt tattacaaaa atgaaggtac tgccgaaaat
     4381 attcagatag agcttaggga tgaccaggat gcgacgttaa aaaatggcga tagcaaaacg
     4441 gttattgttg atgagatcac tcgtaatgca cagtttccac ttaaggcaag agctatcacg
     4501 gtgaatggaa acgcaagcca gggaacgatc gaggcgctaa tcaatgtgat ctacacctgg
     4561 caataacgtc gattcgattt tcttagcctt ctttttaaat caatttttta accttaggat
     4621 atatcatggg taaaacaatc agtataaagg tcctgttcgg tatttatctt ctattaatgg
     4681 caggcaaagt ttttgccttc agttgtaatg tcgatggcgg gtcaagtatt ggagctggta
     4741 caacctctgt ttatgttaat ctcgaccctg taatacagcc gggccagaat ctggttgtag
     4801 acttgtctca gcatataagt tgctggaatg attacggcgg ctggtacgac actgatcata
     4861 taaacctggt acaaggctca gctttcgcag gctctctaca gtcatataaa ggatcgctgt
     4921 actggaataa tgtgacctac cccttcccgt tgacgacaaa taccaacgta ttggatattg
     4981 gtgataagac accgatgcca ttaccgttaa aactctatat taccccagtc ggggcggccg
     5041 gtggagttgt cataaaagcc ggggaagtta ttgcccgtat tcatatgtat aaaattgcca
     5101 cgttaggaag cggaaatccg cgtaatttta cgtggaatat tatttcaaat aacagtgttg
     5161 ttatgccaac aggcggctgc actgttgata gtcgtaatgt caccgtgaat ctcccggact
     5221 ttcctggaag cgcagaaatc ccccttggcg tatattgctc aagcgaacaa aagctctcat
     5281 tttatctttc gggtactacc actgacagtg ctagacaagt gtttgctaac acagcaccag
     5341 atgcaacaaa agcatcggga gtgggtgtct ctctgatgcg taatggaaaa atactggcta
     5401 cgggagaaaa tgtctccctg gggacagtaa ataaaagcaa agtacctctg ggtttaagcg
     5461 caacctatgg acaaacaggg aacaaagttt cagccggaac cgtccagtca gtcattggtg
     5521 tcacttttat ctatgaataa ttgtgatttt gaccttagtc tatgcgctcc attcacgaaa
     5581 attgcgcgga taattatttt gtgaaggcta ttatcctaca cttgtaagaa aatccgcgca
     5641 caattgaagc cagagtctta ttttttatat tgaaaaatat gttaatttaa acactatgag
     5701 agatgatgct taccttatcg tatttaatct attgtttaac gataatagat taaaaataga
     5761 aagagagtag tcccctactc ttatatatcc atgttggcga taatcccttt gtatgtactg
     5821 tgcatcatcg ctattacaaa tcctaataat tcatttccac acaggataag tagatgaaga
     5881 aaaaaattga atcctaccag ggtgctgccg gtggttgggg tgctgttaaa tccgtagcga
     5941 atgcagtacg taagcagatg gatatacgcc aggatgttat tgccatgttt gacatgaata
     6001 agccagaggg ctttgactgt ccgggttgtg catggccaga tcctaagcac agtgcgtcat
     6061 tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga agtcacggat aagcaggtaa
     6121 atgcctcttt ctttgctcag aatacggttc aatcattact tacctgggga gatcacgagc
     6181 ttgaggctgc gggacgtctc actcagcctt tgaaatatga tgctgtcagc gactgttaca
     6241 agccattaag ctggcaacaa gctttcgacg aaattggcgc acgccttcaa agctatagtg
     6301 atcccaatca ggttgaattc tatacttcgg gccgcacttc caatgaagct gcctttcttt
     6361 atcagctttt tgcccgtgaa tacgggagca ataactttcc cgactgctcc aacatgtgcc
     6421 atgaaccgac aagcgtgggt ttggcagcga gtatcggtgt aggtaaaggg accgtgttgc
     6481 tggaagactt tgagaagtgc gatttagtca tttgcattgg gcataaccct ggtacaaacc
     6541 accctcgcat gctgacttcg ttgcgcgctt tagtgaaacg gggagcgaaa atgatcgcca
     6601 tcaatcctct acaggaacgt ggcctggagc gatttaccgc accgcaaaac ccgtttgaaa
     6661 tgctgacgaa ctctgagact cagttggcca gtgcctacta taacgtgcgc attggtggtg
     6721 atatggcgtt gctcaagggg atgatgcgcc tgttaattga gcgcgatgat gctgcaagcg
     6781 ccgcaggtcg gccctcattg ctggatgacg aatttattca aacgcatacc gtcggctttg
     6841 acgagctacg ccgtgacgtg ctcaattccg agtggaaaga tatcgaacgt atttctggac
     6901 taagtcagac acaaatcgcc gaactggctg atgcatatgc cgctgccgaa cgaaccatta
     6961 tctgttacgg aatggggatc actcagcacg aacatggtac ccagaatgta cagcaactgg
     7021 tcaatctgct gttgatgaaa ggtaacattg gcaagcctgg tgcgggtatc tgcccactac
     7081 gtggccactc taatgtacag ggcgaccgaa ccgtcggtat caccgagaaa ccgtctgtag
     7141 agtttctggc tcgcctgggt gagcgctatg gcttcacccc acctcatgta cctggacatg
     7201 ctgcaattgc cagcatgcaa gcaatatgta cggggcaggc tcgagcattg atctgcatgg
     7261 ggggcaactt tgcactggca atgccagatc gggaagcgag cgctgtaccg ttaacgcaat
     7321 tagatttggc ggtacacgta gccactaagc ttaaccgctc tcatctgctg accgcacggc
     7381 atagctatat tctgccggtt ctgggacgta gcgagattga catgcaaaaa agcggtgcgc
     7441 aggcggtaac cgttgaggat tcaatgtcga tgattcatgc ctcgcgtggc gtgttaaaac
     7501 ccgccggtgt aatgctgaaa tcagagtgtg ctgtggtcgc gggaatcgcg caggcaacac
     7561 taccccagag cgtggtagcc tgggagtatc tggtggaaga ttatgatcgc attcgcaatg
     7621 acattgaagc tgtgctgcca gagttcgccg actataacca acgcatccgt catcccggtg
     7681 gttttcacct gataaatgca gctgctgaaa ggcgctggat gacgccgtca ggtaaggcta
     7741 atttcattac cagcaaaggg ctgttagaag atccctcttc agcgtttaac agtaagctgg
     7801 tcatggcgac agtacgcagc cacgatcagt acaacacgac gatttatggt atggatgatc
     7861 gctatcgagg ggtattcggt caacgagatg tggtctttat gagtgctaaa caagctaaaa
     7921 tttgccgtgt aaaaaacggc gaaagagtta atcttattgc gcttacgcca gacggtaagc
     7981 gcagctcacg ccgcatggat agattaaaag tggtcattta ccctatggct gaccgctcac
     8041 tggtgaccta ttttccagaa tcgaatcaca tgctaacact tgataaccac gatccattaa
     8101 gtggcattcc tggctataaa agtattccgg ttgaattaga accatcaaat taatgcctct
     8161 tctcatttct tctgctgtca tccgcacagc agaagaattc ctcattgatt attatcgcgc
     8221 aatttgctca catggattaa attaaactac atactataag atataaactt ctgcctacag
     8281 ctgtaagaaa ctccgctcag tactgaagca ccagtcctat ttcctctttt ctcaagcctg
     8341 ttatattaag catactgatt aacgattttt aacgttatcc gctaaataaa catatttgaa
     8401 atgcatgcga ccacagtgaa aaacaaaatc acgcaaagag acaactataa agaaatcatg
     8461 tctgtaattg tggttatctt attactgacc cttacgttga tagccatttt ttcggcaatt
     8521 gatcagctgg gtatttcaga aatgggtcgc attgcaagag atcttacaca tttcattgtc
     8581 aatagtttgc aagactgaaa ataaacagca aattataaat atgaaatgtt aaaaaagtat
     8641 cgataaaact ttattgtttt aaggagataa aatgtcgctc gtttgttctg ttatatttat
     8701 tcatcatgcc ttcaacgcta acattttaga taaagattac gccttctctg acggcgagat
     8761 cctgatggta gataacgctg ttcgtacgca ttttgaacct tatgagcggc attttaaaga
     8821 aatcggattt aatgaaaata ccattaaaaa atatctacaa tgcactaaca tccagacagt
     8881 gacgatgcct gttcctgcga agtttttacg tgcttcgaat gtaccgactg gattgcttaa
     8941 tgaaatgatt gcttatctca actcggaaga acgcaatcat cataattttt cagaactttt
     9001 gcttttttct tgcctgtcta tttttgccac atgcaaaggt ttcattacac tattaactaa
     9061 cggtgtgcta tccgtttcgg ggaaagtgag aaatattgtc aacatgaagc tggcgcaccc
     9121 atggaagctg aaagatattt gtgactgcct gtacatcagt gaaagcctgt tgaagaaaaa
     9181 actaaagcaa gagcaaacga cattctcaca gattctttta gatgccagaa tgcagcacgc
     9241 aaaaaatttg atacgcgtag aaggttcagt caataaaatt gccgaacaat gtggttatgc
     9301 cagtacatct tattttattt atgcgttccg caaacatttc ggcaacagtc caaagagagt
     9361 ttctaaggag taccgttgtc aaaggcacac gggtatgaat acgggcaaca cgatgaatgc
     9421 tttagctatt tgattatttg ctaacgagta gtcaaccacg caccctacgt aaaaattaaa
     9481 aatggggcag tcattccctg ccccgcgttg tttttaggcg atatatttat tgaaataaat
     9541 aagtga
//
LOCUS       DAZKIF010000154         9529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZKIF010000154 DAZKIF010000000
VERSION     DAZKIF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.155"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHOTW_004052"
     CDS             complement(<1..209)
                     /locus_tag="ACHOTW_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846066.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLD"
     gene            complement(295..564)
                     /locus_tag="ACHOTW_004053"
     CDS             complement(295..564)
                     /locus_tag="ACHOTW_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846067.1"
                     /translation="MNKKQLAILEKAWDAQISYALKEQALPIIQTKSKIARQLCDGGF
                     LNEVEITRQMVTFKGYEINHHGIAAYCSHLPDDVDIDEMEGEMKQ"
     gene            complement(565..3471)
                     /locus_tag="ACHOTW_004054"
     CDS             complement(565..3471)
                     /locus_tag="ACHOTW_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP9846068.1"
                     /translation="MTPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LKVNISGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDAKR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEIVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKKQYLFNPPWESLADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGDV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFELKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQANPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRT
                     RVKTGVTYTYKDDKRHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRN
                     VEKIGNAECLCIMVDHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKP
                     GILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRY
                     GIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTG
                     KMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEK
                     QRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
     gene            complement(3582..4334)
                     /locus_tag="ACHOTW_004055"
     CDS             complement(3582..4334)
                     /locus_tag="ACHOTW_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9846069.1"
                     /translation="MSMNLMAKAMNIKVGNPLRKLVLIKLADNANDNGECWPSYQHVA
                     DQCEVSRSTVKSHIRALEEMGLLKREFRRKGELNQSNVFYLTLDNAQQIQPESGGAGA
                     ARGGAGAAPRTYHSFEPVNEPKNIMFEHVRTECEKTPDRHEETDKAFEEIFWCAGMRK
                     AGKKNAASAFRTQFREWRKTTRGTASEFATMLAEDIACRLGKQFGFDRLLPSSYLNGQ
                     RWNDEKPETIQPQSKPSSAITVSKTGYVFFDR"
     gene            complement(4497..4796)
                     /locus_tag="ACHOTW_004056"
     CDS             complement(4497..4796)
                     /locus_tag="ACHOTW_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP9846070.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSDQIQMEF"
     gene            complement(4935..5162)
                     /locus_tag="ACHOTW_004057"
     CDS             complement(4935..5162)
                     /locus_tag="ACHOTW_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9846071.1"
                     /translation="MQNLDEPIKGVGIPEVAKACGVSERAVYKWLKNGFLPKTEFFGK
                     TKYASKIEEISGGKYQASEMLEISKKNLLAA"
     gene            5241..5948
                     /locus_tag="ACHOTW_004058"
     CDS             5241..5948
                     /locus_tag="ACHOTW_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP9846072.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGANDEDTLSFVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILK
                     QSRHLDDIEAREWLKSS"
     gene            6009..6350
                     /locus_tag="ACHOTW_004059"
     CDS             6009..6350
                     /locus_tag="ACHOTW_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HFP9846073.1"
                     /translation="MAFNDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRANPDCCFFG"
     gene            6418..6879
                     /locus_tag="ACHOTW_004060"
     CDS             6418..6879
                     /locus_tag="ACHOTW_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846074.1"
                     /translation="MRILGVRAAPKVTSFVVYCTNESALKCVDVIKIPSTLDTPEKLK
                     YVRNNILDILNLYNVELAAIRVTESNSDNLSIDRLYIEAVIQEAFSSSDVRKYYTIRK
                     SGMKSSLNLTEIEYKEILKSHRNINGIDNSGFTTETNEAVLAALSAEVRGC"
     gene            6873..7919
                     /locus_tag="ACHOTW_004061"
     CDS             6873..7919
                     /locus_tag="ACHOTW_004061"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HFP9846075.1"
                     /translation="MLTPYKRADVEFEWISDLEEQGCFSKVYLAHDRHLAHDLVIKEI
                     EKKENTNHDDYFNEARLLYKHAHPNIVQVQYAAQCESNIYIAMPFYHNGSLNQLMKKN
                     NLTSREIIRYSIQFLSGLYHIHSKGLMHFDIKPNNIMISNRNEAMLSDFGLSQLVNEE
                     SRAAPEFGYHFHVPPEYFSLSTNDYNFTYDIYQAGLTIYRMCVGHDNFERERSAFSTI
                     EQLRESIINSCYPLKEYPPHIHKKLITIVNKCIHVDPNERYQSVLDVLNDLSAISDGV
                     LDWRLQMTKPTNGTCEWQKKSGDAILSIVFDAENSSTTGFRLYDDGRKRRATNLTISS
                     GCTPTKLYRLLKDN"
     gene            7922..8086
                     /locus_tag="ACHOTW_004062"
     CDS             7922..8086
                     /locus_tag="ACHOTW_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846076.1"
                     /translation="MKKREEVSKLPRRRDAALAVPYKKDEFISPSDDKKFSKAKSFTS
                     TSLKDKYFKI"
     gene            8890..8958
                     /locus_tag="ACHOTW_004063"
                     /pseudo
     CDS             8890..8958
                     /locus_tag="ACHOTW_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            9017..9487
                     /locus_tag="ACHOTW_004064"
     CDS             9017..9487
                     /locus_tag="ACHOTW_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846077.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
BASE COUNT         2641 a         2196 c         2051 g         2641 t
ORIGIN      
        1 aaatccagac tccggataat tctgttgcgc tgaaactcat tgttgagttt gaacaaccgt
       61 cgaagaacac ggtcacgcgg atagcgtcgt gcggcaggtg aatgctcata caactcatca
      121 agcggcaaac tggacgatga acgataccga taccaacgca ccaactcttc acgaaaatta
      181 gccctgacaa gctcagctat cgtactcatt tcttaaagcc tccaatccct ctcccccaaa
      241 taaaaaggcc tgcgattacc agcaggcctg ttacaagctc agtgatgtag atggtcattg
      301 cttcatctcc ccttccattt catcaatgtc aacgtcatca ggaagatggg aacaatacgc
      361 tgctatacca tgatgattta tctcataccc tttgaacgtt accatctggc gcgtaatctc
      421 aacttcgttc aggaatccgc catcgcataa ctgcctggct attttcgatt tggtctggat
      481 tattggtagt gcctgttctt tcaaagcgta tgatatttgt gcatcccatg ccttttcgag
      541 aatggctaat tgttttttat tcatttaata ctccgtcacg ttttcctgtc gccacgcctc
      601 gtcatattcc gatttcggca tattggcgat gtagctatat ggcgatcctg attcaagttg
      661 caggaactgg tgcgattgct cgtcaaggaa caacgggaca ccaccttccc aaccttcgcc
      721 gttacgttgt ttttcaagca tcaaaacaga tgccggagat gccagtagct gttcgtcctt
      781 ctctgacatc ttttcaccac tctgaactct ctgtaacgct ctctcgcgag ccttgttacg
      841 ccagatgatg aaaaggttgt ctgtcaggtc tgttatcgct ccagagcctt ttacgtccat
      901 tttcccggtt ggtttttctt cgctgtctcc ttttcgcgag tgagtaacga gaatgacgtg
      961 ggagtttgtt ttgtttttga agtcgcaaat cgagtcaaca aacgccttct gcccgttata
     1021 gtcatcgtcg cctatgccac atttcatcag gctgtcgatg atgaataact ggataccgta
     1081 tcggcggcga gcgtagtcga atatttcgat cagcctgtcg gctttcgccg ttccggtcag
     1141 gccaaacacc caaagtcttt cgtcataaaa tttaaatgca gagtcaattt ccagcactgg
     1201 cggcatcttg cagcacgtcg cctgacgggt aaggcgctta aggagaatac caggcttcag
     1261 ctcaagtgac gcgatgcacg tcttcacacc ctgacgcatt gcctcaagtg ccatatgccc
     1321 gacaacctcg gtgttatgcg tcgccacata tcccctggtt acatacaggt ggcgagggtg
     1381 atcgaccata atacacaagc attctgcatt gccgattttt tcaacattcc tgacgaatac
     1441 cccacagccc tcgaatcgat gcgcagttaa tctgccattg agtcgtggtg aacggatggc
     1501 ctccctgatt tctcttgtca gtctgattct tgcctcgtat gaatccatcc catgccgctt
     1561 atcgtccttg tatgtgtatg tcacgccagt tttaactcgc gtccggcatg acccgccgag
     1621 tgagttaacc agttgaacaa ccccattgcg cagttcttca cttgctgagg aaaaaacaag
     1681 cgttccatcc ttctcgacat acccatccgt ttcaagcaga ccacacagca tgcctatacg
     1741 cgttgattta tttgcggaaa aaaacacacg agggatgaat ttgttttttg ctgtgcaccc
     1801 cattagtcca taacctcgca gggtctccat gagtggattt gcctgaccac gcgccgttga
     1861 tatcagccag tccttaccat ctccagagaa gttgtaatca ggcagttcag ccttcatacg
     1921 ctcgatcatg tatggctcga cgtttgaaaa cttcacgctc ccattgctaa gactaccatc
     1981 cccgagaagg gagccgataa cccatgctaa tggctctgag tggtcgccaa agtcaccagt
     2041 tatttcagga atcctaacgc catttttgtg cctcttcgtt tcactcaacc gcttcagctc
     2101 gaaggtgtca atcacgcggc gtttctcgcc tttcgtgaat ccacgactag tgacctccca
     2161 taggtgatcg cctgcgcaat caacataacg accatcttca aatgtgactc ggtaaacatc
     2221 tctaacaccc tgcgggaata tcccagtgac agttgacgga ttgccgtcta ctgacgccac
     2281 ctgatcgcca atttttacat ccccgtgagt agtccatgtc ccatcagcta aaagaatagg
     2341 ctcattcagt gggcatgcct ttccgtgacc gttcacacca ttgaccagcg tcaactcggc
     2401 ctcacggaac tggaatttat ctgccagaga ttcccacggt ggattaaaca gatactgctt
     2461 cttgccgtag aaagcgttga tagtgtcctg gtaaaactct cgcgcactgt agagttcttc
     2521 aggatcgaaa taggatgccg tgccgatgta ctgccagatt tcatcctcgg taacaccgtt
     2581 catcaggcat tcgttgatgt ctttgtacgg cagagtaaca agacggcaac gatgttcacc
     2641 gagtcggctt gcgatttccc ttgcggcttc acgaccaaca tcatcaacgt ccatcgagat
     2701 gaatatttcc tcaaacctgt cgaggttgtg atactcaaac tcaatccact gctgcttagc
     2761 gcctttcccg ccaccaaacg gcacggataa cgccgagatg ccgtattgcg catagctcat
     2821 acaatcaatt tcgccttcgc aaagtacaac cgccctcacg ccagcgtcca gagcctgcca
     2881 tccgaacaga caaggttcgc aatcaccttc tgccataatg actttcttcc cgtccgggcg
     2941 ctcagtgctg attcgcttga cctgcaacaa ctcaccatcg cgtttgtacg gaagcaccag
     3001 agcatccagt tcccgctctc cattccacac cttgccgctg acaacctcgt agcgctttac
     3061 gatttctggc gatatgccac gcgattgcag gtactcaaga tgggattctg ttctggtaac
     3121 gtagcgggcg attttcttgc ggtcaggtct ggagaatttt ttctcacgtt tggcatcgaa
     3181 atggtgatcg tcatccttga taccgagaaa tgctttcgct tcctgcatag cctgatgcag
     3241 gttaattcca cgacatgcca tccacaaatc aagcatgtca ccgccgtctc cctcagcgaa
     3301 atcagcccat tttttcttgc cgctaatgtt gaccttaagg ctgtttccct tgtcaccgtt
     3361 gacgttaccg gcaacccact catgcccctc tttcttgccg tttggcaaca ggtgcggagc
     3421 caccctgtca acctgcgccc aaagcaggtc gctcagttca cttggagtca ttacgctgac
     3481 ctcagatcga gacggttaaa ccagaactca acgaatgcag aactaagcca gccatggtta
     3541 tagccagcga taagtaacga tttgattctg gatttcatgg ttcacctgtc gaaaaacacg
     3601 tagccagttt tcgatacggt gattgcggat gatggtttgg attgtggttg aatagtttct
     3661 ggcttctcgt cgttccagcg ttgaccgttc aggtagctcg atggtaacaa cctgtcgaac
     3721 ccgaactgct taccgagcct gcacgcaatg tcttctgcca gcatcgtggc aaactcgctt
     3781 gccgtccccc tggtagtttt acgccattcc ctgaactgtg ttctgaatgc tgaagctgcg
     3841 tttttcttcc cggctttccg catgcctgca caccagaata tttcctcgaa tgccttgtcg
     3901 gtttcttcgt gacggtcagg agttttttca cactctgttc ggacatgttc gaacataatg
     3961 tttttaggtt cattgactgg ttcaaaagag tgataggttc tgggggcagc tcctgcccca
     4021 ccccgggcag ctcctgcccc acctgattct ggttggattt gttgtgcatt atccagcgtc
     4081 agataaaaaa cgtttgactg gttaagctct ccttttcttc tgaattccct tttcaaaagc
     4141 cccatctctt ccagtgccct aatgtgactt tttactgtcg atctgctcac ctcacactgg
     4201 tcagcgacat gttgatatga aggccagcat tcgccattat cattggcgtt atcggcaagt
     4261 ttaatcagaa ccagttttct cagtgggttg ccaaccttta tattcatggc cttagccata
     4321 agattcatgc tcattttgac ttctccgaag ttttgtacct gttaagtatt tctctcagtg
     4381 gcacagctat tgctggatta accccctgat aaaactggtc acgtagcaca tcttttcggt
     4441 gattaacgcg tttattttcc tgcgtttttc gcatataatt acctcgttgg atgttgttaa
     4501 aattccattt gtatttgatc agaacgctcg gtcttgcaca ccgggcgttt tttattggtg
     4561 agtccatcaa gcgcatactt aaaagccctg ctaatcggac tgatgtctga tgccattccg
     4621 aaagcacaca agaccgaagc aataaacctc cagtccgttc tgcttatctt cgattcatga
     4681 cagccaatca tctttgccag accacgctgg gtaagcgttg acaggttgat gagtaaatca
     4741 gtttctgcgc gatcaacgtc acgctgggat agtttgctgt aacttgtttg ttccatttct
     4801 taagatttcc aatagtgaat agttagttga aaggtatgcg tggaaacgca tatggcctta
     4861 gttggtcaga tatattggga ctcgctttgt cagcgacgta ggacgaatgt ccattgtgaa
     4921 aatagcggtg ttacttatgc agccagaagg ttctttttgc ttatttcaag catttcgctt
     4981 gcttgatatt tgccaccaga aatctcttcg atttttgatg cgtatttagt tttcccaaaa
     5041 aactcagtct tagggaggaa gccgtttttg agccacttat agacagccct ttcgctaact
     5101 ccacaagcct tcgcaacttc agggatgccg acacctttaa tcggctcatc aagattttgc
     5161 ataggaatat cctttttcgt actttcagta cgtattatgg ttgaactgaa agtttttgca
     5221 agtgctttag tatcgtactc atggttcaga atgaaaaagt gcgcaaagaa ttcgcccagc
     5281 ggctagcgca agcctgtaaa gaagctggtc ttgatgaaca tggtagggga atggctatag
     5341 cccgtgccct ttctctttcg tccaaaggcg ttagcaaatg gtttaatgct gagtctttac
     5401 cgcgtcagga aaaaatgaat gcgcttgcga aatttctaaa cgttgatgtt gtttggcttc
     5461 agcacggcac ttcgttaaat ggagcgaatg atgaagatac tctttcattt gttggcaaat
     5521 taaaaaaagg gttagtgcgc gtggttggtg aggcaattct tggtgttgat ggtgccatcg
     5581 agatgaccga agagcgcgat gggtggctca aaatttatag cgatgatcca gatgcctttg
     5641 gtcttcgtgt gaaaggagac agcatgtggc ccagaataaa atcaggagaa tatgtactca
     5701 ttgagcctaa caccaaagta ttcccgggtg atgaggtgtt tgtcagaacc gttgaaggac
     5761 acaacatgat taaggttctt ggctatgaca gagatggaga ataccaattt acaagcatta
     5821 accaggatca caggcctata acgttgcctt atcatcaagt agcaaaggtg gagtatgtag
     5881 ctggtattct gaagcaatct cgccatctgg atgacatcga ggcaagggag tggctgaaaa
     5941 gttcgtgact tcatcgtcac atagctggta accagtggcc tgaagagacg tttgggtaag
     6001 gaggatagat ggcgttcaat gaccttgaat atcaagcagt aaaaaaagaa gttcaccaat
     6061 tcattgaaag cataaggccg cctgaacata tccgcaatga actggatatt gtttatagca
     6121 tcaatgacca aacgatagat atcggcgaac agcgccccgt gtggcagggc aatccaggtg
     6181 aaacaaacat cctgccatca gcaagaatca agtacatacg ttctctggat agatggaaaa
     6241 tctattggat gcggaaggat atgaaatggc atcagtacag tactgaactt tcgctgactg
     6301 atgcgcttga gcttgtgcgt gctaacccgg attgctgctt cttcggatga gtgaagagac
     6361 gtttggatga tggatggtcg cagagatgcg gcctgattct aaaataggat ataaaaaatg
     6421 agaatactag gtgttagagc ggcgcccaaa gttacatctt ttgttgtata ttgcactaat
     6481 gagtctgcac tcaaatgtgt tgatgttatt aaaatacctt cgaccttaga cacaccagaa
     6541 aaattaaagt atgtgagaaa taacatcctc gacattctta atttatataa tgttgaatta
     6601 gctgccatac gcgttactga atcaaactct gataatctta gcattgaccg cctttatata
     6661 gaagctgtta ttcaagaagc attttcaagc agtgatgtaa gaaaatatta cactattaga
     6721 aaatctggca tgaaatcatc attgaaccta acagagatcg agtataaaga aatattgaag
     6781 tcacaccgca atataaatgg aatcgataat tctggtttta caactgaaac aaatgaagct
     6841 gttttggctg cactatctgc ggaggtaagg ggatgctaac tccatacaaa agagctgatg
     6901 tagaattcga atggattagt gatctagaag aacagggttg tttttcaaaa gtatatctgg
     6961 ctcatgacag acacctagct catgacttgg tgattaaaga aatagaaaaa aaagaaaaca
     7021 ctaaccacga cgactacttt aatgaagcaa ggcttctcta taaacatgca catccaaata
     7081 ttgtgcaagt tcagtatgct gctcaatgtg agagcaatat ctatatagcc atgccatttt
     7141 atcataatgg ttcgctaaac caattaatga aaaaaaataa tcttacaagc agggagataa
     7201 tacggtattc cattcaattt ttaagtggac tttatcatat acactcaaaa ggtcttatgc
     7261 attttgatat aaaacctaat aacattatga tatcaaacag aaatgaggcc atgctatctg
     7321 actttggatt atctcagtta gtcaatgagg aatcgagagc tgcgcctgag tttggatatc
     7381 attttcatgt gccaccggaa tatttttctt tatcaacaaa tgattataat ttcacatatg
     7441 acatatatca ggcaggatta accatatata gaatgtgtgt tggacatgat aattttgaaa
     7501 gagaaagatc tgcatttagc acgattgaac aactcagaga gtcgataatt aatagctgct
     7561 atccattaaa agagtatcct ccccatatac ataaaaaatt aataacaata gtgaacaaat
     7621 gcattcatgt agatccaaat gaaagatatc aatccgtact agatgtacta aacgatctct
     7681 cagctataag tgatggcgtt cttgactggc gtctacagat gacgaaacca actaacggca
     7741 catgcgaatg gcaaaaaaag tctggggacg ctatactgtc tatagttttt gacgcagaaa
     7801 attcgtctac tactggtttt cgtttatacg atgatgggcg gaaaaggcgt gctacgaact
     7861 taacaatatc ctcaggatgt acccctacaa aactgtatag gttattaaag gataactgat
     7921 catgaaaaag cgcgaggaag taagcaagct gcctcgcaga cgtgatgcag cattagcggt
     7981 tccctacaaa aaagatgagt tcataagccc ttctgatgac aaaaaatttt caaaggcgaa
     8041 aagttttaca tctacatctc taaaagataa atactttaaa atctagcccg gcctcagcgc
     8101 cgggttttct ttgcctcacg ttcgcccacc taaaaaacat aaccaattgt atttattgat
     8161 gtaactcgct aaaccatgca gttatgatcc ctgccgcata accttcatca gccacatttt
     8221 caaaaataaa tttccttata tatcagaatc atacttcgta gagttaataa atcaccaaaa
     8281 ttcgtaccaa tagttcttga taatgtcgaa ctattggttc attattatcg tcgtcagcag
     8341 gacgcattac tcaccagggc ggtgaatata caacgattcg aatatgaatc tacggcgctg
     8401 acaaagcgca ataaccaaag tgaactttgg ggtgtggtga agggttcatg gacgggaata
     8461 tgtcgcacgt aaagcggcga ggcctgcggg actattgccg aattgaagta ggccgaaaca
     8521 ggtcgaaatg ggtctcccac ctaccacacc accaaagttc atcaggaggt ctatatgaca
     8581 cgcagaactc agttcaaagg caattcacgt tctcgtcgtc gtgagcgttt aaaggcaaag
     8641 gcattagcta acggcgtact ggcccgcgaa gaagcaataa gttcagaagt attacaccgc
     8701 cctactctaa gcagagcgca gattcaggct aaaggtactc acgaaacgcc tgagcgcata
     8761 gaagacgcta agccaattaa gttcatggca caggacgtga tctggcaaca gaaagaatac
     8821 agacgcaatc tggagcgagc ggccattgtg tacgcgaatg agtttggaca taagcaacca
     8881 gaaactggtg tatgtcttcc aaacgtagcc atttacgcgg caggctaccg gaaatcaaaa
     8941 caactgacgg cgaggtgact tgtgttggtc gccagaaaat gaaattaggc agcaaaccac
     9001 ttatttgagg tgagatatga caaaatcatg gagcgtacct tttcctgaat cagaaactga
     9061 acatgatgga atgcctgttt tctggagatt ccaggcgaca gttgaagaag atgggataaa
     9121 aatattcgca cttcaatata tagcttttca tcagacagag cattatgcat ggttggttcc
     9181 tgcgcattgg attgttaatt ttaaaccagc accaaatcag tggttacagg aatggaaaca
     9241 aaggagaaat agatatgcaa ttaagaaagt agcaaaaaat gcagaaagat cttttgcatt
     9301 cccaacgaag aaacttgcca ttgaaagttt attgcgccgg aagaaatacc atttaatgag
     9361 aatcaaacaa gatttggctg ttgtatcaac tcttgttgat ggtatgaaga atattgatac
     9421 atcaacacca gatattgaat ataactttgg acacaaccaa gaaacagaaa attgggtatt
     9481 ttattagtac gaataagcac tgtgtattca ttccaacgag tgaatacac
//
LOCUS       DAZKIF010000155         9371 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZKIF010000155 DAZKIF010000000
VERSION     DAZKIF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.156"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..234
                     /locus_tag="ACHOTW_004065"
     CDS             <1..234
                     /locus_tag="ACHOTW_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9846078.1"
                     /translation="GKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLD
                     HLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            206..2134
                     /locus_tag="ACHOTW_004066"
     CDS             206..2134
                     /locus_tag="ACHOTW_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9846079.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGGGGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRSIMFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPSPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2118..2324
                     /locus_tag="ACHOTW_004067"
     CDS             2118..2324
                     /locus_tag="ACHOTW_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP9846080.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2321..3913
                     /locus_tag="ACHOTW_004068"
     CDS             2321..3913
                     /locus_tag="ACHOTW_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9846081.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3903..5408
                     /locus_tag="ACHOTW_004069"
     CDS             3903..5408
                     /locus_tag="ACHOTW_004069"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9846082.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5445..5792
                     /locus_tag="ACHOTW_004070"
     CDS             5445..5792
                     /locus_tag="ACHOTW_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9846083.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            5850..6101
                     /locus_tag="ACHOTW_004071"
                     /pseudo
     CDS             5850..6101
                     /locus_tag="ACHOTW_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            6098..6838
                     /locus_tag="ACHOTW_004072"
     CDS             6098..6838
                     /locus_tag="ACHOTW_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9846084.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            6852..7283
                     /locus_tag="ACHOTW_004073"
     CDS             6852..7283
                     /locus_tag="ACHOTW_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9846085.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            7310..7723
                     /locus_tag="ACHOTW_004074"
     CDS             7310..7723
                     /locus_tag="ACHOTW_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9846086.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            7704..>9371
                     /locus_tag="ACHOTW_004075"
     CDS             7704..>9371
                     /locus_tag="ACHOTW_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9846087.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKFLLAHKDETLEYKRQLAELGDKVE"
BASE COUNT         2163 a         2288 c         2961 g         1959 t
ORIGIN      
        1 ggaaaactga ttgacaccgg gttctgtatt tttgccctca gtaagctggc aatggcgtta
       61 tccagtacgc ttgattccat ccctttatcc atgcagcgac agtttcctga tttaacaccg
      121 cgccatcttg accatctgaa aacccttatt gctaaggggg caaatcagtg tgcgcgggca
      181 ggggataaat taccggattt acttgatgaa tatatcagag caacaactga ataatatgat
      241 gagcgctgtc acaactgcat tacagcccct gataagggca ttgccggtga cgccagttga
      301 atgggctgat caaaattatt atctgcctaa agaatcttca tatggtgagg gcgaatggaa
      361 aacgctgcca ttccagatcg ccatcatgaa cagtatgggg aatgatcaga tccgcactgt
      421 taatctgatt aaatctgccc gtgttggcta tacaaagatg ttgctggggg ggggcgggta
      481 ttttattgag cataaatccc gaaacagtct gctttttcag cccacggatt ctgccgctga
      541 agattttatg aagtctcacg tggaggcgac gattcggaac gtgccatgcc tgaaagacct
      601 ttccccatgg ctgggtcgta aacatcgtga caatactctc acgctgaaac gcttttcatc
      661 gggcgtcggt ttctggtgcc tgggcggcgc tgccgccaaa aactaccgtg aaaaatccgt
      721 tgacgtggtc tgctatgacg aactttcctc gttcgagccg gatgtcgaaa aagagggctc
      781 gccaaccctg ctgggggata agcgtattga ggggtcggtg tggccaaaat ccattcgcgg
      841 ctcgacgcct aaaatcaaag gcacctgcca gatcgaaaaa gccgctaacg agtcggcgca
      901 ttttatgcgt ttttatgtgc cctgcccgca ctgtggggag gcgcagtatc tgaaatttgg
      961 cgatgagtcc acgccttttg ggcttaaatg ggagaaggac agccctgaaa gtgttttcta
     1021 cctctgtgaa catcatggct gcgtgatcca tcagtctgaa ctggaccaga gcaacgggcg
     1081 gtggatctgt gaaaacacgg gcatgtggac ccgtgacggt ctgacgtttt tcagcgcccg
     1141 gggtgatgaa attccgccgc cgcgctccat catgttccat atctggacgg cgtacagtcc
     1201 gttcaccacc tgggtacaga ttgtctatga ctggctggat gcactgaaag atcccaacgg
     1261 cctgaaaacc tttgtgaaca ccacgctggg cgagacctgg gaagaggccg tgggcgaaaa
     1321 actcgatcac caggtactga tggataaggt ggtgcgttac acggcggcgg tgcctgcccg
     1381 ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga tgtatgtctg
     1441 gggatgggct ccgggagagg aagcctttct ggtggataaa atcatcatta tggggcgtcc
     1501 tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aacaaaaaat accgccatgc
     1561 ggatggcacc gaaatgacta tttcccgtgt ctgctgggac accgggggga tcgatggtga
     1621 aattgtttat cagagatcaa aaaaacacgg tgttttccgg gtgctgccgg taaaaggcgc
     1681 atctgtctat ggcaagccgg tgatcaccat gccaaaaacc cgcaatcagc ggggcgtgta
     1741 tctgtgtgaa gtgggaacgg acaccgcaaa agaaattctc tatgcccgta tgaaagccga
     1801 tccctcgcct gcggatgaag ccacgtcgta tgccatccgt tttcctgatg atccggagat
     1861 tttttcgcag acagaggcgc agcaactggt ggcggaagag ctggtggaga agtgggaaaa
     1921 aggaaagatg cgtctgctgt gggataacaa aaagcggcgt aacgaagcgc tggactgcct
     1981 ggtgtatgcc tacgcggcat tacgtgtgtc cgtgcaacgc tggcagcttg atctggctgt
     2041 actggcaaaa tcccgggaag aagagacgac ccggccaacc ctgaaagaac tggcagcgaa
     2101 gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt caggcgcgcc
     2161 ttgatttatt aaccggtaaa cgggtggtgt ctgtccagaa agatggtcgc agaattgaat
     2221 atacggcggc ttctctggat gagcttaacc gggcgatcaa tgatgcggag tcggtactgg
     2281 ggacaacccg gcgtcgccgt cgtccgctgg gagtgaggtt atgaaacgaa cgcctgtcct
     2341 gattgatgtg aacggcgttc cgcttcgtga gagtctcagc tacaacgggg gcggtgcagg
     2401 atttggcggg caaatggcgg agtggttgcc accggcgcag agtgccgatg cggccctgct
     2461 gcccgcgttg cgtctgggga atgcccgggc agatgatctg gtgcgcaata acggaatagc
     2521 ggccaatgcg gtggcactgc ataaggatca cattgtcggg catatgtttc ttatcagcta
     2581 ccgtccgaac tggcgctggc tggggatgcg ggagaccgca gcaaaaagct ttgtcgatga
     2641 ggtggaggcg gcctggtcgg aatacgccga agggatgtct ggcgagatcg acgtggaagg
     2701 aaaacgcacg ttcacggaat ttatccgtga aggtgtgggc gttcatgcgt ttaacggcga
     2761 aatctttgtg cagccggtct gggatacgga aaccacgcag ttattccgta cgcgttttaa
     2821 agccgtgagt ccgaaacggg tggacacgcc aggacacggt atggggaacc gttttctgcg
     2881 ggccggggtg gaggtcgatc gatatggccg tgccgttgcg taccatatct gtgaggatga
     2941 ttttcctcgc tccgggagtg gacgatggga acggatcccg cgtgaacttc ccaccgggcg
     3001 tccggccatg ctgcatattt tcgagccggt ggaggacggg cagacccgtg gggccaacca
     3061 gttttacagc gtcatggaac ggctgaagat gctcgattcc ctgcaggcaa cacagcttca
     3121 gtcggccatt gtgaaagcca tgtatgcagc gacgattgaa agtgaccttg ataccgaaaa
     3181 ggcctttgaa tatatcgccg gtgcgccgca ggggcagaag gataatccgc ttattaatat
     3241 tctggagaag ttctccagct ggtatgacac gaataacgtg acgctgggtg gtgtcaaaat
     3301 tccgcacctt ttccccgggg atgatctgaa actacagact gcgcaggatt cagacaatgg
     3361 attttcggcg cttgaacagg cgctgctgcg gtatatcgcc gccggtcttg gcgtttccta
     3421 cgaacagttg tcccgtgatt actcgaaggt cagttattca agtgccaggg cctctgccaa
     3481 tgagtcgtgg cgctatttta tggggcggcg aaaatttatt gcggcccggc tggccacgca
     3541 gatgttttcc tactggctgg aagaggcact tcttcggggg attatccgtc cgccacgggc
     3601 gcgttttgat ttttatcagg cgcgatcagc ctggtcacgg gcagagtgga ttggtgccgg
     3661 aagaatggcc attgacgggc tcaaggaggt tcaggaatcg gtgatgcgca ttgaggccgg
     3721 actgagcacg tatgagaaag agccggcgct gatgggcgag gattatcagg acattttccg
     3781 ccagcaggtc agggaatctg cagagcggca aaaagccgga ctctcacgtc cggtgtggat
     3841 agcgcaggcg tatcagcagc agatagcgga gagtcgcagg ccggaagagg agacaacacc
     3901 ccgtgagacg taatctttca cacattattg ccgcagcatt caatgaaccg ctgcttctgg
     3961 agcccgccta tgcgcgggtt ttcttttgcg cgctcgggcg cgagatgggg gcagcaagtc
     4021 tttcggtacc acagcagcag gtacagcttg atgctcccgg aatgctggct gaaacggacg
     4081 agtacatggc cggaggtaaa cgaccggccc gtgtttaccg ggtggtgaac ggtattgctg
     4141 tactgccggt gaccggcacg ctggtgcacc ggctgggggg tatgcggcca ttttccggaa
     4201 tgacaggcta tgacggcatt gtcgcctgtc ttcagcaggc aatggcggat agccaggtgc
     4261 ggggcgtact gctggacatt gacagtccgg gcgggcaggc cgccggcgcg tttgactgcg
     4321 ctgacatgat ttaccgcctc cgtcagcaga agccggtctg ggcactgtgc aatgacacgg
     4381 cctgttctgc agccatgctg ctggcgtcgg cctgctcccg acggctggtt acccagacat
     4441 cccgtatcgg ctccattggc gtgatgatga gccatgtcag ctatgccggt catctggcgc
     4501 aggccggtgt ggatatcacg ctgatttact caggggcgca caaggtggat ggcaatcagt
     4561 ttgaagcctt accggcagag gttcgccaga acatgcagca gcgcattgat gcggcgcgcc
     4621 ggatgtttgc cgaaaaagtg gccatgttta ccggtctgtc tgttgatgcc gtcacgggaa
     4681 cagaggccgc cgtttttgaa ggtcagtccg gcattgatgc cgggctggcg gatgaattag
     4741 tcaatgcgtc ggatgccatc agtgtgatgg ccacggcgct gaacagtaat gtcagaggag
     4801 gcactatgcc gcaattaact gcaacggaag ccgccgcgca ggagaaccag cgagtgatgg
     4861 ggatcctgac atgccaggaa gcgaaaggac gtgaacagct tgccacgatg ctggcaggac
     4921 aacagggcat gagcgttgaa caggcccggg cgattctggc cgcggcggca ccgcagcagc
     4981 cggtggcatc cacgcagagt gaagccgatc gcattatggc gtgtgaagaa gcgaacggtc
     5041 gtgaacaact ggcggcaacg ctggcggcga tgccggagat gacggtggaa aaagcccgcc
     5101 cgatcctggc tgcttcaccg caggcggatg ccggaccctc actccgtgat cagatcatgg
     5161 cactggatga ggcaaaaggg gctgaggcgc aggctgaaca gctggctgcc tgcccgggaa
     5221 tgactgtgga gagcgcccgg gctgtgctgg ctgcgggatc aggtaaggca gaaccggtct
     5281 ctgcatccac aaccgccctg tttgaacgca tcatggcgaa ccattcaccg gctgcggtac
     5341 agggtggcgt gccacagacg tcagcagacg gtgatgcgga cgtgaaaatg ctcatggcca
     5401 tgccatgaag tcagtgctga ccatcaacag gaggttttta caatatggta acgaaaaaca
     5461 tcactgaaca gcgtgcggaa gtacgtattt ttgccggtaa tgatccggct cataccgcca
     5521 caggcagcag cgggatttcc tcggcaacac cggcactgac gcccctgatg ctggatgaag
     5581 ccagcgggaa actggtggtc tgggacggac agaaagccgg tagtgcagtt ggcatactgg
     5641 tactgccgct tgaaggcaca gagacggtac tgacctatta caagtcgggg acctttgcga
     5701 cggaggcaat ccgctggcct gaaagtgtgg atgaacacaa aaaggcaaat gcctttgccg
     5761 gcagtgccct gagtcacgcg gcgctgccgt aacacgttat caggccaccg cggtggcctg
     5821 actgatttct gaatgaaagg aactgattta tgggattgtt tacgacccgc cagttactcg
     5881 gttataccga acaaaaagtg aaatttcgtg cgctgtttct ggagctgttt ttccgccgta
     5941 cggtgaattt ccataccgaa gaggtgatgc tggacaaaat taccggaaaa acgccggtgg
     6001 cggcctatgt ctccccggtt gttgaaggaa aagtgctgcg tcatcgcggt ggtgaaaccc
     6061 gcgtgttacg tccgggctac gtcaagccga aacacgaatt cccctggagc cggtaaaagg
     6121 agccggtacc accctgtggg tttataacgg tcagggtgac gcctatgcaa acccgttgtc
     6181 agacgatgac tggcagcgac tggctaaggt gaaggatctg acgccgggcg agatgacggc
     6241 agaatcctac gatgataact acctggatga tgaagacgcg gactggaccg cgaccgggca
     6301 ggggcagaaa tctgcaggtg ataccagttt tacgctggcc tggaaaccgg gagaggaagg
     6361 ccagaaaggg cttataggct ggtttgaaag cggcgatgtc cgggcctata aaatccgttt
     6421 tccgaatggc acggtggatg tgtttcgtgg ctgggtcagc agtatcggta aggccgtgac
     6481 ggcgaaagaa gtgatcaccc gcacggtgaa agtcactaac gtgggtaaac cttctgtagc
     6541 ggaagaacgc agcaaaatta cgccggtcag tgcgattaag gtgacgccga catccggtac
     6601 ggtggcaaaa gggaaaacaa ccaccctgac ggtttctttt gagccggaaa gtgcaacaga
     6661 caagacgttc agagcggttt ccgccgatcc gtcgaaagcc accattagtg tgaaagatat
     6721 gacaattacg gtaaacggcg tggcgacagg taaggtgcag atccctgtgg tgagcggaaa
     6781 tggtcagttc gccgcagtgg ctgaagtcac cgttactgaa gcgggcgctg cagggtaaac
     6841 ggaggtcata catgtttctg aaaacagaac aatttgaata taacggtgtg tctgtcacgc
     6901 tttccgaatt gtctgcgctg cagcgtatcg agcatcttgc cctcctgaaa cggcgtgcag
     6961 aacaggcaga atccagcggc aacctgcagg taagcgtgga agatctcgtc agaaccggcg
     7021 cgtttctggt ggcgatgtcc ctgtggcata accatccaca gaaaacgcag tcaccgtcaa
     7081 tgaatgaggc cgtgatgaag atagagcagg aagtgctcac cacctggcct gccgatgcca
     7141 ttgcccgggc ggaagacgtg gtgttgtgcc tgtccgggat gagcggggct gttcgtccgg
     7201 atactgatat tactgaagtg gcgaaaaata acacgctgac tgatgatgat ttttctgcgg
     7261 gaaagtcttc gacggcgagc tgaactttgc cctcagactg gcgcgtgaga tggggagacc
     7321 cgactggcgc gccatgcttg ccgggatgac atccaccgaa tatgccgact ggcaccgttt
     7381 ttaccgcacg cattattttc aggataccca gctggatatg catttttccg ggctgacgta
     7441 cgctgtactc agcctgtttt tttgcgatcc ggatatgcat ccctctgatt tcagtctgct
     7501 tgtcccccgg catgaggaag agcaggtgga gaggccggat gaggacaaaa tgctgatgca
     7561 gaaagcggca ggacttgccg gaggcgtccg gttcggtggg gacggagggc gcgatatttt
     7621 atcgtctgcg gatgtggcgg atgtcatggt ggatgatgcc gcattaatga tggcttcagc
     7681 ggggattccg ggaggtgtga gatatgtccc agccggttgg tgatcttgtt attgacctga
     7741 gtctggatgc tgtccgtttc gatgagcaga tgagccgggt aaggcgtcat ttttcaggtc
     7801 tggataccga cgtcagaaaa accgccagtg ctgttgaaca gggcctgagc cgccaggcgc
     7861 tggctgcaca aaaagccggg atttccgtcg ggcagtataa agcggccatg cgaaccctgc
     7921 ccgcacagtt tacggatatc gccacgcagc ttgccggtgg tcagaatccc tggctgatcc
     7981 tgctgcaaca gggcggtcag gtgaaggact ccttcggcgg gatgatcccc atgttcaggg
     8041 ggcttgccgg tgcgatcacc ctgccgatgg tcggggtcac ctcgctggcg gtggcgacag
     8101 gtgcgctggt gtacgcctgg taccagggag attccacgct ttcagcgttt aataaaaccc
     8161 tggttctttc cggtaatcag tccggactga ctgccgatcg catgctgacg ctctcaagag
     8221 ccgggcaggc agcagggctg acgtttaacc aggcgagaga gtcactggca gccctggtga
     8281 atgccggtgt gcgtggtggt gaacagtttg atgccatcaa ccagagtgtc gcgcgttttg
     8341 cttctgcatc cggtgtggag gtggacaagg ttgcagaggc tttcggaaaa ctgaccaccg
     8401 accctacgtc ggggctgatt gcgatggtgc gccagttccg taacgtgacg gcagagcaga
     8461 ttgcgtatgt tgcgcagctg cagcgttccg gtgatgaggc cggggcctta caggcggcga
     8521 acgatatcgc cacgaaaggc tttgatgagc agacccgtcg cctgaaagaa aacatgggga
     8581 cactggagac ctgggcggat aaaaccggga aggcattcaa atcgatgtgg gatgccattc
     8641 tggatatcgg tcgtcctgag tcctcagcgg atatgctcgc cagtgcacag aaggcatttg
     8701 atgaggcgga taaaaaatgg cagtggtacc agagccggag ccagcgccgg ggaaaaaccg
     8761 cctctttccg ggccaacctt cagggcgcat ggaatgaccg ggaaaatgcc cgtctggggc
     8821 tggcagcggc cacgctgcag tcggatatgg aaaaagccgg tgaactggcc gccagggacc
     8881 gggccgaacg ggacgcatca cagctgaagt ataccggaga ggcgcagaag gcgtatgagc
     8941 gtctgctgac gccgctggag aaatataccg cccgtcagga agaactgaat aaggccctga
     9001 aagacgggaa aatcctgcgg gcggattaca acacgctgat ggcggcggcg aaaaaggatt
     9061 atgaatcgac gctgaaaaag ccgaagtcgt caggagtcaa agtgtcagcc ggtgagcgtc
     9121 aggaagacca ggcgcatgct gccctgctgg cgcttgaaac cgagctcagg acgctggaaa
     9181 aacacagcgg tgcgaatgag aaaatcagcc agcagcgtcg cgatttatgg aaagcggaaa
     9241 atcagtatgc ggtcctgaaa gaggctgcca cgaaacggca gttatctgag caggaaaaat
     9301 tcctgctggc gcataaagac gagacgctgg agtacaaacg ccagctggct gagctgggag
     9361 acaaagttga a
//
LOCUS       DAZKIF010000156         9370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZKIF010000156 DAZKIF010000000
VERSION     DAZKIF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.157"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            133..1530
                     /gene="ulaA"
                     /locus_tag="ACHOTW_004076"
     CDS             133..1530
                     /gene="ulaA"
                     /locus_tag="ACHOTW_004076"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9846088.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1546..1851
                     /gene="ulaB"
                     /locus_tag="ACHOTW_004077"
     CDS             1546..1851
                     /gene="ulaB"
                     /locus_tag="ACHOTW_004077"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP9846089.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1861..2325
                     /gene="ulaC"
                     /locus_tag="ACHOTW_004078"
     CDS             1861..2325
                     /gene="ulaC"
                     /locus_tag="ACHOTW_004078"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP9846090.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2339..2989
                     /gene="ulaD"
                     /locus_tag="ACHOTW_004079"
     CDS             2339..2989
                     /gene="ulaD"
                     /locus_tag="ACHOTW_004079"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9846091.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2999..3853
                     /gene="ulaE"
                     /locus_tag="ACHOTW_004080"
     CDS             2999..3853
                     /gene="ulaE"
                     /locus_tag="ACHOTW_004080"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP9846092.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3853..4539
                     /gene="ulaF"
                     /locus_tag="ACHOTW_004081"
     CDS             3853..4539
                     /gene="ulaF"
                     /locus_tag="ACHOTW_004081"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP9846093.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4668..4943)
                     /gene="yjfY"
                     /locus_tag="ACHOTW_004082"
     CDS             complement(4668..4943)
                     /gene="yjfY"
                     /locus_tag="ACHOTW_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP9846094.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5270..5665
                     /gene="rpsF"
                     /locus_tag="ACHOTW_004083"
     CDS             5270..5665
                     /gene="rpsF"
                     /locus_tag="ACHOTW_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9846095.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5672..5986
                     /gene="priB"
                     /locus_tag="ACHOTW_004084"
     CDS             5672..5986
                     /gene="priB"
                     /locus_tag="ACHOTW_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP9846096.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5991..6218
                     /gene="rpsR"
                     /locus_tag="ACHOTW_004085"
     CDS             5991..6218
                     /gene="rpsR"
                     /locus_tag="ACHOTW_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9846097.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6260..6709
                     /gene="rplI"
                     /locus_tag="ACHOTW_004086"
     CDS             6260..6709
                     /gene="rplI"
                     /locus_tag="ACHOTW_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9846098.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6780..7574)
                     /gene="yjfZ"
                     /locus_tag="ACHOTW_004087"
     CDS             complement(6780..7574)
                     /gene="yjfZ"
                     /locus_tag="ACHOTW_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP9846099.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            7846..8139
                     /locus_tag="ACHOTW_004088"
                     /pseudo
     CDS             7846..8139
                     /locus_tag="ACHOTW_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(8197..8628)
                     /gene="tnpA"
                     /locus_tag="ACHOTW_004089"
     CDS             complement(8197..8628)
                     /gene="tnpA"
                     /locus_tag="ACHOTW_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HFP9846100.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            8636..>9370
                     /locus_tag="ACHOTW_004090"
     CDS             8636..>9370
                     /locus_tag="ACHOTW_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9846101.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANI"
BASE COUNT         2332 a         2234 c         2478 g         2326 t
ORIGIN      
        1 gtggaattat ttgcgggtcg cgtcacattt aatcataaat aatcttgttg tgattacttt
       61 tgaaaattag agtgagtgca caacattccg ggtgtgtgga atacccggtt acctcttctt
      121 caggagatcg ttatggagat cctctacaac atctttaccg tattttttaa ccaggtcatg
      181 accaatgccc cgttgttgct gggtattgtg acctgtctgg gctacatcct actgcgcaaa
      241 agtgtcagcg ttattattaa aggcacgatt aaaaccataa ttggtttcat gttgttgcag
      301 gcagggtccg gcatcctcac cagcaccttc aaaccggtgg tggcgaaaat gtccgaagtc
      361 tacggcatta acggcgcaat ttccgatacc tacgcttcaa tgatggcaac catcgaccgc
      421 atgggcgatg cctatagctg ggtgggttac gccgtattgt tagcgctggc actgaacatc
      481 tgttacgtgc tgttgcgtcg cattaccggc attcgcacaa tcatgttgac cggccacatc
      541 atgttccagc aggccgggtt gattgccgtt acgctgttta tcttcggcta ctccatgtgg
      601 accaccatta tctgtaccgc gattctggtt tcgctctact ggggcatcac ttccaacatg
      661 atgtacaagc cgactcagga agtgacggat ggctgtggtt tctccatcgg tcaccagcag
      721 cagtttgcat catggattgc ctataaagtc gcgccgttcc tcggcaaaaa agaggagagc
      781 gttgaagacc tcaaactgcc gggctggctg aacattttcc acgacaacat cgtctccacg
      841 gcgattgtga tgaccatctt ctttggcgcc attctgctct ccttcggtat cgacaccgtg
      901 caggcgatgg cgggcaaagt gcactggacg gtctacatcc tgcaaaccgg tttctccttc
      961 gcggtggcga tcttcatcat cactcagggc gtgcgcatgt ttgtggcgga actctctgaa
     1021 gcatttaacg gtatctccca gcgcctgatc ccaggtgcgg ttctggcgat tgactgtgcg
     1081 gctatctata gcttcgcgcc gaacgccgtg gtctggggct ttatgtgggg caccatcggt
     1141 cagctgattg cagttggcat cctggtcgcc tgcggctctt ccattctgat tattctaggc
     1201 tttatcccga tgttcttctc caacgccacc atcggcgtgt tcgctaacca cttcggcggc
     1261 tggcgtgcag cgctgaagat ttgtctggtg atgggaatga tcgaaatctt tggttgcgtc
     1321 tgggcggtga aactcaccgg tatgagtgcc tggatgggca tggcggactg gtcgattctg
     1381 gcaccgccga tgatgcaagg cttcttctcc atcggtatcg cctttatggc cgtcatcatt
     1441 gtaattgcac tggcttatat gttcttcgct ggccgcgcgc tgcgcgcaga agaagatgca
     1501 gaaaaacaac tggcagaaca gtctgcttaa taaggagttt tgattatgac cgtacgtatt
     1561 ctggctgtgt gtggcaacgg acaaggcagt tccatgatca tgaagatgaa agtggaccag
     1621 tttttaaccc aatcaaacat tgaccatacg gtaaacagct gcgcggttgg cgagtacaaa
     1681 agcgagttga gtggcgcgga tatcatcatc gcttctacgc acattgcggg cgaaatcacc
     1741 gtgaccggca acaaatacgt ggttggcgtg cgcaacatgc tctctcctgc cgactttggc
     1801 ccgaaactgc tggaagtgat caaagagcat ttcccgcagg acgtgaagta aggacgcgcc
     1861 atgaaattac gtgattcgct ggctgaaaat aaatccatcc gtctgcaggc tgaagcagag
     1921 acatggcagg aagcggtgaa aatcggcgtt gacctgctgg tggcggcaga tgtggtagag
     1981 ccgcgttact accaggcgat tctggatggc gttgaacagt tcggtcctta tttcgttatc
     2041 gctccgggcc tggcaatgcc gcacgggcgt ccggaagagg gcgttaagaa aaccggtttc
     2101 tctctggtaa cgctgaaaaa gccgctggag ttcaaccacg atgacaacga tccggtggat
     2161 atcctcatca ccatggcggc ggtcgatgcc aatactcacc aggaagtggg catcatgcag
     2221 atcgtcaacc tgtttgaaga tgaagagaat tttgaccgtt tacgcgcctg ccgtaccgag
     2281 caggaagtac tggatctcat tgaccgcacc aacgcggcag cttaagaagg aattgaacat
     2341 gtcattaccg atgttgcaag tcgcgctgga caaccagact atggatagcg cctacgaaac
     2401 cactcgcctg attgccgaag aagtcgacat tatcgaagtg ggcaccattc tgtgcgtggg
     2461 cgaaggcgtg cgtgcggttc gtgacctgaa agcgctctac ccgcacaaaa tcgtactggc
     2521 agacgccaaa attgccgatg caggcaaaat cctttcgcgt atgtgcttcg aagccaacgc
     2581 tgactgggtg acggtaattt gctgtgcgga tatcaacacc gccaaaggcg cgctggacgt
     2641 ggcaaaagag tttaacggcg acgtgcagat cgaactgacc ggttactgga cctgggaaca
     2701 ggcgcaacag tggcgcgacg caggtattca gcaggtggtt tatcaccgca gccgtgacgc
     2761 gcaggccgca ggcgtggcgt ggggcgaagc ggacatcacc gcgatcaaac gtctttccga
     2821 tatgggcttc aaagtcaccg tcactggtgg cctggcgctg gaagatctgc cgctgttcaa
     2881 aggcattccg attcacgtct ttatcgcggg ccgtagtatc cgtgatgccg cttctccggt
     2941 ggaagccgca cgtcagttca aacgttccat cgccgaactg tggggctaag gagcggatat
     3001 gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg ctccccgccg gggagtgctg
     3061 gctggaacgc ctgcaactgg caaaaacgtt gggcttcgat tttgtcgaga tgtcggtaga
     3121 tgaaacggac gagcgcctgt cgcgcctcga ctggagccgc gagcagcgtc tggcgctggt
     3181 gaatgcgatt gttgaaaccg gcgtgcgcgt gccgtccatg tgcctttctg ctcatcgtcg
     3241 tttcccgctg ggcagtgaag atgacgcggt gcgggcgcag gggctggaga ttatgcgtaa
     3301 agctatccag ttcgcccagg acgttggtat tcgcgtgatc caactggcgg gctatgacgt
     3361 ttactatcag gaagccaata acgaaacgcg tcgtcgtttc cgtgacggcc tgaaagagag
     3421 cgttgagatg gcgagccgcg cgcaggtgac gctggcgatg gagatcatgg attatccgtt
     3481 gatgaactcc atcagcaagg cgctgggcta cgcgcactat ctcaacaatc cgtggttcca
     3541 gctctacccg gatatcggca acctgtcggc gtgggacaac gatgtgcaga tggagttgca
     3601 ggccggaatc gggcatatcg tcgcggtaca tgtgaaagac accaaaccgg gcgtcttcaa
     3661 aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt tgcttcgaaa cgctcaaaca
     3721 gagtggctat tgcgggccgt acctgattga gatgtggagc gaaacggcgg aagacccggc
     3781 ggcagaagtg gcgaaagcgc gtgattgggt gaaagcgcgc atggcgaaag cgggcatggt
     3841 ggaggcggca taatgcaaaa gctaaaacag caggtgtttg aagccaacat ggatctgccg
     3901 cgctatgggc tggtgacctt tacctggggc aacgtcagcg ctatcgaccg cgaacgcggg
     3961 ctggtggtga tcaagcccag cggcgtcgcc tacgaaacca tgaaagcaga cgatatggtg
     4021 gtggtcgata tgagcggtaa ggtggtggaa ggggaatatc gtccgtcttc cgacaccgcg
     4081 acgcatctcg aactctaccg tcgttatccg tcgcttggcg gcattgtcca tacccactcc
     4141 actcatgcta ccgcatgggc gcaggcgggg ctggcgatcc cggcgttagg caccacgcac
     4201 gctgactact tctttggtga tatcccgtgt acgcgtggat taagcgaaga agaggtgcag
     4261 ggtgagtatg aactgaacac tggcaaagtg attatcgaaa cgctgggcaa cgccgagccg
     4321 ctgcatacgc cgggaattgt ggtgtatcag cacggaccgt tcgcctgggg gaaagatgcc
     4381 cacgacgcgg tgcataacgc ggtagtgatg gaagaagtgg cgaaaatggc gtggattgcg
     4441 cgcagtatta atccacaact caatcacatc gacagcttcc tgatgaataa acacttcatg
     4501 cgtaagcacg gccccaacgc ttattacggg cagaagtaga acacgcgctg cggaaatttc
     4561 cttcctcggg agataactgg tctaattccg cagccgtttt tcaaaaaaag ccccctgcga
     4621 agggggcaaa gcaaactatg gcaatgtttc gttggttata cctggtgcta gcgataaata
     4681 tccgcgctgg cgtgcatatt gccgttactc cccggttccc gcatcagaat tacgtggtag
     4741 tacgttgcgc cttgcgcatc tgtttcttca tttaatgcct gacgtgcttc gctttcagtg
     4801 gcgaaattat gattgatata aatcacgcct aagctttgca catcgtccat atttctggcc
     4861 tggtggttat taatttcaat ggctgcccat gtatttgcac ttagcaaaag cacagccaga
     4921 agggctaaaa ctcgactgaa catagatacc tcctcgacgg ctgactttgt gtgctctcct
     4981 ctgtgatgat cttctgattt aattttaatc aatgataaag aagttgatgg tgaccatttc
     5041 tgatgcagtt gttcgaaaaa acaccatgat gaagtgtgat gaacttcaaa tcagcgtgtt
     5101 agtccacttt ggtgaaagat gagatttatt tgtcggctgg ccttgagttt agcggggtat
     5161 gtcagtacta taacgcgtca tttttcagcc gacctttaac acgttccttg cctccccggg
     5221 attcggctga cccagacagg aggctgaata atccgtaagg agcaattcga tgcgtcatta
     5281 cgaaatcgtt tttatggtcc atcctgacca gagcgaacag gttccgggca tgatcgagcg
     5341 ctacactgct gccatcactg gtgcagaagg caagatccac cgtctggaag actggggccg
     5401 ccgtcagctg gcttacccga tcaacaaact gcacaaagct cactacgttc tgatgaacgt
     5461 tgaagctccg caggaagtga tcgatgagct ggaaactacc ttccgcttca acgatgccgt
     5521 tatccgcagc atggttatgc gtaccaagca cgctgttacc gaagcatctc cgatggttaa
     5581 agcgaaagac gagcgccgtg agcgtcgcga tgatttcgca aacgaaaccg ctgatgatgc
     5641 tgaagctggg gattctgaag agtaatttct gatgaccaac cgtctggtgt tgtccggcac
     5701 cgtgtgcagg actccccttc gaaaggtcag tccatcagga attcctcact gccagttcgt
     5761 gcttgagcat cgttctgtgc aggaggaagc cggctttcac cggcaggcat ggtgtcaaat
     5821 gcccgttatt gttagcggac acgaaaacca ggccattact cacagtataa cggtcggcag
     5881 tcgcataacc gttcaggggt tcatttcatg ccacaaggca aagaacggac tgagcaaaat
     5941 ggttttgcat gccgagcaga ttgaattgat agattctgga gactagccat atggcacgtt
     6001 atttccgtcg tcgcaagttc tgccgtttca ccgcggaagg cgttcaagag atcgactata
     6061 aagatatcgc tacgctgaaa aactacatca ccgaaagcgg taagattgtc ccaagccgta
     6121 tcaccggtac ccgtgcaaaa taccagcgtc agctggctcg cgctatcaaa cgcgctcgct
     6181 acctgtccct gctgccgtac actgatcgcc atcagtaatc ggtcacggtc cattaatacg
     6241 actttgagag gataaggtaa tgcaagttat tctgcttgat aaagtagcaa acctgggtag
     6301 cctgggtgat caggtaaacg ttaaagcggg ctatgctcgt aacttcctgg taccacaggg
     6361 taaagctgtt ccagctacca agaaaaacat tgaattcttc gaagcacgtc gcgctgaact
     6421 ggaagctaaa ctggctgaag ttctggcagc tgctaatgct cgcgctgaga aaatcaatgc
     6481 actggaaact gttaccatcg cgtctaaagc tggcgacgaa ggtaaactgt tcggttccat
     6541 cggtactcgc gacatcgctg acgctgtaac tgcagctggc gttgaagtgg ctaagagcga
     6601 agttcgtctg ccgaacggcg ttctgcgtac cactggcgaa cacgaagtga gcttccaggt
     6661 tcacagcgaa gtattcgcga aagtgatcgt aaacgtagta gctgaataat tcgttattca
     6721 acgagacgta aaaacgccga ccactggtcg gcgttttgct ttctattttt cgtcaggtat
     6781 tagtttcgca agtagatcca ttcaccattc tcttgtgtcc ataagtcagg atggtttgct
     6841 ggcgttagat acatacgagc gcaactgatg tctcgctcct gcggcactcg ctcgaaaccc
     6901 aaacccgagt agattgcggc tgaacttgat aaggaacgca gaccaatttt caaatcatgg
     6961 ggtaaatcag aatacgcgtt tagcaatgca gcaatgatga atccgagggc attagaaacg
     7021 cctgtaacag tgcgaagttg gtcgatataa agatcgattg ttggtagttc agatgaccag
     7081 caattaggca tttgggcagg aagggttaca acgtatccta tcgtttggcc ggtatagtct
     7141 ggagtaccat aaccaggtcc caaaaccact tcgtgtattg tgtatttact gttgggttcg
     7201 aaggattcga catcttgcaa taaatatttc agttctgcgg ttaacctcgc acccgcgtaa
     7261 gctttattgt atttctgggc gtgaatatta tctaatgtag cttcatggtt ccatccaaaa
     7321 gaattatcac cattcattgt tactccatcg taagcattac acttcgttgc taacttattg
     7381 aatgcttgcc agtctgagag atctagtttg tggatctcaa cagtacgtct gccatcgcaa
     7441 ttatgatctc ttccacgatt tgaaagtaat gtagcgcgtg cgtattgatg aaattttaat
     7501 tttactggtt tgtcggtgga cgaaaaacga gataaatatg caggaaatga ataaatagtg
     7561 gttggtaatg tcattgtaat gtgacctgtt taatgttttt ataaatatcg cagctgaatg
     7621 ttcacagaaa aaaatcgata ttaataaaaa taagtttgtt ttctgaagtg gtgaaaatag
     7681 cggatggtga aagatattaa tatcaaaaat cgctcattat ggatataacg atgcacccgg
     7741 ggagaaaatg acttccacgc cttacaccgt acattcttgt tgtgcggtat gtaattattc
     7801 tacgctgtcg gtatcggtgt attcgttaat cagcaggtta accagccaca aaaagccatt
     7861 gagaaaatta ttgattttac atgggattat tatattgcta acccctggtt tttaaaaatt
     7921 gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa aatcacagcg tctcttagaa
     7981 atcaaccatg cgcacttgca gttaatggaa tccttattgg atgaagggaa gaaatacaat
     8041 atttttaagc cagatatcga cccattgcag gtgaatatta atattgctgc gcttggggga
     8101 tattatttga tcaaccagca tacgcttggc ctggtttatc cggtgcgccg taaaaccccg
     8161 tccttcaggg cggggatata cggcgcggtt ttccacctaa ccaggtgttt gctgttgttc
     8221 aatgtattga cggatggcgg atattggcgc acctccgcag ctactggcaa aatagccagg
     8281 actccacaaa acgcctttgt agtaatacct gactgcaata tctggtcgat ctcgtcgcag
     8341 taacctaccc gatacgcctt tgaggctgtt taccagactg gatatcgcca gtttgggagg
     8401 atagttaatt aacaaatgga cgtgatctgg ttcgccatcc atttcaacca gttcagcttc
     8461 aaaatcagca catacctttg aaaagtaagt gcgtagtttt tctgtcgcgt catggtcaaa
     8521 aatctggcgt cggtatctgg tgacaaagac caggtgaaca tgcatcagga aaacacaatg
     8581 ccttccacgc cggatatcgg tttctttttt cacagaccaa agtatcattt ggcctgtgaa
     8641 acgactacag gcatttaaat tccagttaag acccggtggt caacaggagc gtgaaatgag
     8701 gcgcttcgca ggcgcatgtc gtttcgtttt caatcgtgct ctggcgcttc agaatgagaa
     8761 tcatgaggcc ggtaataaat acatccctta cgggaaaatg gcttcctggc tggttgagtg
     8821 gaaaaatgcc actgaaacgc aatggcttaa agatgccccg tcacagccat tgcaacagtc
     8881 actgaaagac cttgagcggg cttacaaaaa cttcttccgg aagcgggctg cttttccccg
     8941 attcaaaaag cggggacaga atgatgcatt ccgctacccg cagggcgtta agctcgatca
     9001 ggaaaacagc cgtatttttc tgccgaaact tggctggatg cgctaccgga atagccgtca
     9061 ggtcacgggt gttgtgaaaa atgtcactgc cagccagtcc tgcggtaagt ggtacatcag
     9121 tattcagact gaaaatgaag tatcaactcc ggttcaccct tcagcattaa tggtcggact
     9181 ggatgctggc gtggctaaac tcgccacgct gtcagatggc acagtctttg gacctgtaaa
     9241 cagttttcag aaaaaccaga agacgctggc gagacttcag cgacagttaa gccgcaaggt
     9301 caaattcagc aacaactggc agaagcagaa acgcaaaata cagcgactgc attcctgtat
     9361 cgcaaatatc
//
LOCUS       DAZKIF010000157         9283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZKIF010000157 DAZKIF010000000
VERSION     DAZKIF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.158"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(16..516)
                     /locus_tag="ACHOTW_004091"
                     /pseudo
     CDS             complement(16..516)
                     /locus_tag="ACHOTW_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(1175..1357)
                     /locus_tag="ACHOTW_004092"
     CDS             complement(1175..1357)
                     /locus_tag="ACHOTW_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846102.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            1296..3386
                     /gene="iha"
                     /locus_tag="ACHOTW_004093"
     CDS             1296..3386
                     /gene="iha"
                     /locus_tag="ACHOTW_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP9846103.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(3604..3754)
                     /locus_tag="ACHOTW_004094"
                     /pseudo
     CDS             complement(3604..3754)
                     /locus_tag="ACHOTW_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3981..4286)
                     /locus_tag="ACHOTW_004095"
                     /pseudo
     CDS             complement(3981..4286)
                     /locus_tag="ACHOTW_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(4708..5283)
                     /locus_tag="ACHOTW_004096"
     CDS             complement(4708..5283)
                     /locus_tag="ACHOTW_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9846104.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(5352..5930)
                     /gene="terD"
                     /locus_tag="ACHOTW_004097"
     CDS             complement(5352..5930)
                     /gene="terD"
                     /locus_tag="ACHOTW_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFP9846105.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(5979..7019)
                     /gene="terC"
                     /locus_tag="ACHOTW_004098"
     CDS             complement(5979..7019)
                     /gene="terC"
                     /locus_tag="ACHOTW_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFP9846106.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(7042..7497)
                     /locus_tag="ACHOTW_004099"
     CDS             complement(7042..7497)
                     /locus_tag="ACHOTW_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9846107.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(7520..8677)
                     /locus_tag="ACHOTW_004100"
     CDS             complement(7520..8677)
                     /locus_tag="ACHOTW_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9846108.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(8677..9258)
                     /locus_tag="ACHOTW_004101"
     CDS             complement(8677..9258)
                     /locus_tag="ACHOTW_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9846109.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         2414 a         2301 c         2258 g         2310 t
ORIGIN      
        1 tggataaaga ttctaacaaa gcatttcaac aatatatact tgttaaaaat ccatatcgaa
       61 atgccgttgc agcattaata tatgcctcat tcataaaatg taaaagagca agctcgtacc
      121 agtagggggg aaattcattg acatgtcctg tcaatacgta cagagccctg ccatgcttgc
      181 cagcacgtaa caattccgcc cccagtaacc agcgtgttcc ctgattattc tcagggttat
      241 atgttagtat tttatcaatg agtataacgg catcctgatg acgctgtaaa tgaacattcg
      301 ccagaattgc agcataaaga gcgcaaatgc atatgccaga tgagcgtgaa tatcaatgat
      361 gtcaggagca tgtcaggcaa gacgcctgag tttgttgacg taatttgttt cgtttatttc
      421 atcactatcg tatgcgtcaa gagttttttc aaactcatcc cgaaatgggc caagcctggt
      481 ttcaggaaat aagaaatacc cttggttatt gttcacttaa aagtataatt tgtaattcaa
      541 atcctgagcc tgtaaagcgg ggagtaacat attctattct gaagagaata aaagtcgtga
      601 tgcgatgtat caagcccgga ttgtaatccc agattaacat agatcacaaa acaacttatt
      661 cttcactaac gtcaagataa atatcgttgt atgccttatc acgactacga ataccagcaa
      721 gatacaactg acatacggaa agataccacg ttttttactc ccgaaaataa cgctaaaaag
      781 ctacttcccc atcgtttgtc cttagtattg ccagcgccaa caatgtgggc tgacatgata
      841 aagctgtcta ggaaattgtt cgcctcctca gcggacaatc caaatggtga ttgtctctgt
      901 taaacgttta ttttgaaggt cgactgaata aggtgatgac gctgtagaat ttttcacgtg
      961 ccacagaatt ttgaacgctt tctcttacaa tatttcaatg tttctatcag tattcgccgg
     1021 aagaagtcat cgaccaaatc atcccagtcg tctcgcatca ctgaccattc atggttgaca
     1081 tgtggtggaa atccgcttct acagtaacca ttttttattc gcaaaaccga acacgccata
     1141 cggatagctg ttaactggca tgccgaggca gtcgttattt atatttggtt ttgtcaataa
     1201 tctttatttt ttgtaaaagg caaatataaa ttattctcat tattgtttgt atttgtgtat
     1261 tgtcttgccg gttaacatga tcggagatta gtaatatgcg aataaccact ctggcttccg
     1321 tagtcattcc ctgtctcgga ttttcagcca gcagcatagc tgctgcagag gatgtgatga
     1381 ttgtctcggc atccggctat gagaaaaagc tgactaacgc agccgccagt gtttctgtga
     1441 ttagccagga ggaattgcag tccagccagt accacgatct ggcggaggct ctgagatcag
     1501 tagagggtgt ggatgttgaa agtggtacgg gtaaaaccgg agggctggaa atcagcatcc
     1561 gaggaatgcc agccagttac acgctgatac tgattgatgg tgttcgtcag ggcggaagca
     1621 gtgacgtgac tcccaacggt ttttctgcca tgaataccgg gttcatgccc cctctggccg
     1681 ccattgagcg tattgaggtt atcagggggc cgatgtccac actgtatggc tctgatgcga
     1741 tgggcggtgt ggtgaatatc attaccagaa agaatgcaga caaatggctc tcttccgtca
     1801 atgcagggct gaatctgcag gaaagcaaca aatggggtaa cagcagccag tttaatttct
     1861 ggagcagtgg tccccttgtg gatgattctg tcagcctgca ggtacgcggt agcacacaac
     1921 agcgtcaggg ttcatcggtc acatcactga gcgatacagc agccacgcgt attccttatc
     1981 ccacggagtc acagaattat aatcttggtg cacgtcttga ctggaaggcg tcggagcagg
     2041 atgtgctctg gtttgatatg gataccaccc ggcagcgtta tgataaccgg gatgggcaac
     2101 tggggagtct gacgggggga tatgaccgga ccctgcgcta tgagcgaaac aaaatttcag
     2161 ctggctatga tcatactttc accttcggaa catggaaatc gtatctgaac tggaacgaga
     2221 cagaaaataa aggtcgtgag cttgtacgca gtgtactgaa gcgcgacaaa tgggggcttg
     2281 ccggtcagcc gcgggagctt aaggaatcga accttatcct gaattcatta ctgcttaccc
     2341 ctctgggaga atctcatctg gttacggtgg ggggcgagtt tcagagctcg tccatgaaag
     2401 acggagttgt ccttgccagc acaggtgaaa ctttccggca gaaaagctgg tcggtatttg
     2461 ctgaggatga gtggcatctc acggatgcac ttgcgctgac tgcgggcagc cgctatgaac
     2521 atcatgagca attcggggga cacttcagtc cgcgtgcata tctggtctgg gatgtggcag
     2581 atgcctggac gctgaaaggc ggtgtgacca cgggatataa ggcacccaga atggggcagc
     2641 tacataaagg gattagtggt gtgtccgggc agggaaaaac aaatctactt ggtaaccccg
     2701 acctgaagcc ggaagagagc gtcagttatg aggctggggt gtattacgat aaccccgccg
     2761 gtctgaatgc caatgtcaca ggttttatga ctgacttctc caacaagatt gtctcttatt
     2821 ccataaatga taacaccaat agctatgtaa acagcggaaa ggcccggttg cacggtgtgg
     2881 aatttgccgg cacattgccg ctgtggtcag aggatgtcac gctgtcactg aattacacct
     2941 ggacccgaag tgaacaacgt gatggtgata acaaaggtgc gccgctgagt tatacccctg
     3001 aacacatggt gaatgcgaaa ctgaactggc agatcaccga agaggtggca tcatggctgg
     3061 gtgcccgtta tcgcgggaaa acaccacgtt tcacccagaa ttattcgtca ctgagcgctg
     3121 tacagaagaa agtgtatgat gagaaaggag aatacctgaa agcctggacg gtggtggatg
     3181 caggtctgtc gtggaagatg acggatgccc tgacgctgaa tgctgcggtg aataacctgc
     3241 tcaacaagga ttacagtgac gtgagcctgt acagtgccgg taagagtacg ctgtatgccg
     3301 gtgattactt ccagacggga tcatcaacaa caggatatgt gatacctgag cgaaattact
     3361 ggatgtcgct gaactatcag ttctgataat aacaaaacgc tatcactgac ggtagaatac
     3421 gttgccactg caactcctgg cggaacagtg gcaacgtatt aggttaagtg catttccgat
     3481 ccgctaatga gatttcgtta ccaacaacta atatcgtcac aggaaatgca cggattattt
     3541 ttaacttatc atttacatac ttgtccagag tgtaagcgca ccgtgaagga cgtggggtaa
     3601 aaattagttt acagagagag tgacgttcca gggcaacaac tctttcatgc ggttggcagg
     3661 ccaggtgttg attacactga tcacgtggcg tagccacgtt tccggctcga ttccgttaag
     3721 ttttgcagct accgatcagg ctgtacatca ctgccgcact atcgctcgtc atctcaaagt
     3781 cctgtctcgt cagcaggaag gtatcattct ctcccgccat ttttccaggg gcccggtcag
     3841 ataagtccct ttgtctatcg ctgactcctg actcataacc cggttagcag aatgcagggt
     3901 caccacctcg ccacgaccaa atccaaataa gtcaattgca ccttctcaat cgccattttg
     3961 tcagtaagcg tacagcctca actgatggta tcttcaccat caatgacaac ggtgatcgca
     4021 attttactga cgttcgccgg aacacgatcc agtgctatct caatgctggc ctgctgcgaa
     4081 ccggtaacga gcctgactgc cccctcagga gaagacaaat tattataaaa gataaagtca
     4141 gaatcgccac tgacctttcc ctgagcatta agcatgaaca ggcaggtatc gggttcgcct
     4201 ttaaagccgg atttggcagg gtactgaaga ttcagcctga tcgtagattg ctgaaggggt
     4261 atgttttgtt cggattgtaa attcatatta actctcctga tttttgatta ttattaatgc
     4321 gcagcgttta tatatgttcc ctaggcttag ttctggacgc tggatattcg gtgaggcgta
     4381 aatatggtat gacaccattt ttcataacgc tgaagtttct atacctgttg aatttgaatt
     4441 ttcattgacc gggtatctta ttttccaggg cccgttcctt cataagtcgc aaaagtaaca
     4501 tatatccgaa gggcatgctg ttgatatcag acacggaata ctggctttaa ccagcaacca
     4561 cagataaacc cggggcctgc ataaaaggtt catgccagaa ttaacatagc tctgctttct
     4621 gcccccccct ttcccatatt cacccgttga tagcggatcg atacccaaaa aaaaccccgg
     4681 cattgccggg gtcagactca gcgtctttta gacattgacg ccgtgctggg aagcaagcgc
     4741 tgacagacca ccggcaaaac cctggccaac agctttaaac ttccactcag taccatggcg
     4801 atacagttca ccgaagacca ttgcggtttc ggttgaggcg tcttcagaca gatcgaaacg
     4861 ggcaatttcc gtcccgttgt cgttgttgta aacgcgcatg aagctgttgc tcaccatgcc
     4921 gaagttttgt ttacgcgctt ctgcatcata gatggtaacg gcaaatacca gttttttgat
     4981 gtctgctgag actttggtca gatcgatttt gacctgctca tcgtcgccgt cgccttcacc
     5041 ggtacggttg tcgccctggt gctctactgc gccatcaggg ctggttttat tattgaagaa
     5101 aatgaaatgg gcatctgaca gtactttacc gtcttcacct actgcgaata cggaagcgtc
     5161 cagatcaaaa ccctgaccat cggttacacg ggcatcccag cccaggccaa ccatagcgac
     5221 attcatggtt ggtgcttctt tggtcagaga tacgttgccg ccttttacga gagaaactgc
     5281 catttttagc tcctgcaaac agttgaatga ggctgaatta acagccccag atatgaaaag
     5341 ttacttttcg atcaggacgc gttaatgccg tactgagcac atacagatgc cagaccacca
     5401 gcataaccct ggcctactgc gcggaatttc cactcaccat tgtggcgata cagctcgccg
     5461 aacagcatgg cagtctcagt ggacgcatct tcggtcagat cgtagcgagc gacttcagtc
     5521 tggttatcgt cattaaccag acgaataaac gcaccggata cctgaccaaa gctctggcga
     5581 cgagcctgag catcgtggat ggtcacaacg aagatgatct tgtcaacttc agacgggacg
     5641 gcgtccagtt taattttcag cgattcatca tcaccatcgc cctcaccggt gcggttatcg
     5701 ccggtgtgcg ttacggaacc gtcggatgac gtcaggttgt tatagaagat gaaatctgaa
     5761 tcgccgcgca ctttgccgtt tgaggccagc aggaatgctg aagcatccag gtcaaagtcc
     5821 tgaccgtctg ttgaacgcgc atcccagcca aggcccacca ggacattttt cattgacgga
     5881 gctgctttac tcagggagac gttcccgcct ttggaaagag aaacactcat aaaataacct
     5941 cttcgattag taattgttca ggttaacact taaggggatt aactcccctt ttcctcagat
     6001 tcaggtttgc ccgggaacat gacgcttgcg agaatgccca gcgccagtac acccagtaca
     6061 acatacaggc tggttgttgc cgcgatgctg taaccatgat gccagatgtg atcggtcgca
     6121 ttcaggccga gttttgccgc gatgaagaac agcagcacga taacggcctt ctccagatga
     6181 accaggtact gtttcagtgc ctcaaggaca aaatacagag tacgcagacc caggatagca
     6241 aacatcatgg cactatagac gataagcggt tcacgactga cggcaatgat tgccggtacc
     6301 gagtcaaacg cgaacattac gtccgagagt tcaaccacag ccacacacag gaacagcggg
     6361 gtcgcataca gcgccgcttt tttaccacgg ccaatggtga catcgctgtt ttctggtttc
     6421 gccagttcag catccacttc cttctggtta agtaggaagg catgccctct gagcttcggc
     6481 cagataggga agaagcgttt aaccatacgg taagccagat gctgggagta atcctcaatt
     6541 tcatcatcgt catcaccgct tttaagcatc atgaccgccg tccaggcaac gataatagcg
     6601 aagacaactt caacgtacgg ccccagactc agcaggctcg taccgatggc gacaaagatg
     6661 cccctgaaga cgatggcacc aatgatcccc cagtagagaa cacggtggcg ataacgatcc
     6721 ggaacggcga accaggagaa gattgccatc atgacgaaca ggttatcgac cgacagcact
     6781 ttctccagcg cataacccgt gacaaacaga ctggcaacct cagcaccgtg gtggatatag
     6841 aggaaaccgg caaatgccat cgcaaccaca acccagaata cggaccagag cgcagcactc
     6901 ttcagcgaga ttggcttgtc atcacggtgc ataaacaggt caataaagat ggcaccgact
     6961 gaaagcgcaa taaaaacaat gaccgtttca gtcgggaagc cgatgtgtgt ggaaaccata
     7021 ttttaccctt agggatgtgg atcagaggcc aaattcagcc gggtcgaagc ccaacgcgat
     7081 agcgatctcg cgggaggcca gtttctcgtc ctgatcgaag ttaccgtcac ttttcgcaac
     7141 ggcaatacca acacgtaagg ccagctgagc ggcctcaggc tgatctttca gcgccaggat
     7201 gtatttcatg gtttcgccct tgccaatttc aacatcgaaa tcgaagcttg aaaccagttt
     7261 attgaagaac tcaatcacct cattggtatc gaagaccttc agctcttctg aagagcgcag
     7321 aaagcccatc attttctgct tttcttccga acttacgccg tcactcgata cggcaatacg
     7381 ggcacataca gcaacggtgc cctgcatgaa ttttttgttt ttgaaacggc caacctggcg
     7441 ggtcagttcg tcacggcctg agttaatggc acctttaact ttgtcgaaaa agctcatacc
     7501 tgtttccttt gttgctgtgt tatttagaac cggcgctcca gcgaaatccc cagccaaatg
     7561 cccggtccat ttcgtcctgg cctttgaagt actggttaat tcgctcgact ttaattgcgc
     7621 cgttttcgtt tacgagtctg gcaatggcgc acaatgtgcg acggttctca ccttcggtca
     7681 ggcgggtctc aatgggcggt tgatccggca catgaatggt gactacacca tcagtcttgt
     7741 cccagctggg aacaccttca taaataaagg cgtaaatcag cacttcgcgg atatgcttcc
     7801 attcacgtcc attgatatgc agccactcgc cgtctgatac atcacccgtc cggtcatcgc
     7861 ccttgagctg aacatacggt tcatcgcgat aatcaccgaa tgcattaccg agcgcctgaa
     7921 tgaccgattt atacccgtcc tgaagctcaa caaaggcccc cagatccagg tcgattcctt
     7981 tggagccgaa catacctgca aagcctgatt tgctactgcc ccggtgccag ttcaggttga
     8041 tccggatttc accgaagtta tcccgtttgg tcaggctgat tgcaggcttc tcttttgtca
     8101 gcgatacctt gctcaggctg atttttgtct caactggcgg aggggttaca accggaggag
     8161 ttggggctgc gggagccggt tcgtcggcaa tatttacgcc gaaatgctct gccagcggtt
     8221 taagtccacc gttgaacccc tgcgcaacga agcggaattt ccagtcatta ttacgacggt
     8281 aaaattcccc cagaattaag gctgcttcct gacggccgct cagttcgaca ctgccgctaa
     8341 ccaggccggt agccccttgc tccacgtcga tggaaagatt acgaagaccg gacaccgtct
     8401 gcccaccgtc acaggtcacg gtgaacgcaa ttttctggac atcaggtttc agccggttga
     8461 gcgcgacagt aaaggttgag tactggcctt cactgaccag gctgacggta ccatcatcgt
     8521 tacgtggctg accataaaac accatgtccg catcaccctg caccttcccg tcggcataca
     8581 ggcgaaacgc cgaagcatcc acctggccgc ccgaggtgat ccggacccgc aactcctggg
     8641 ctggaacggg ggcattgccc cccggcgtca ggttcattag cgaataaccg ctgaaacgat
     8701 atcggagtgc atatcgtcaa ttgtgcggcc gcgacaggca tggccaaggg cggtaaagtc
     8761 ccagttgccg ttattacggc gcagcgaaga aataacgatc ccggtatgtg agccctgctc
     8821 agtcagcttg tagcgagcca gctctttgcc ggtttgatcg acaacgcggc agaaagcgtt
     8881 ttcgacgtca ttgaacgact gaccacggaa gctgtttacc gtaaacgcca ggtattcaac
     8941 gtttgcaggc aggcgggaga ggttgacctt aatcacttca tcgtcaccgt caccctcacc
     9001 ggtcaggtta tcgccactgt gcacaacagc gccgcaggtg gatttcagtt tgcggaacca
     9061 gatggtgtca attgttttac ctgtgctgtc cattaataca cagccagcat cgagatcgat
     9121 tgagtcgttg cctccaaaca atcctccaag aagacctttt ttcttaaccg gatcccaacc
     9181 gagaccgaaa tgaagctggc ttaatgcaga tgattctttg ctgagtgata ccgtctggtt
     9241 cttaactaat gaaaccatgt tttattcctt aaaatggtaa tga
//
LOCUS       DAZKIF010000158         8721 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZKIF010000158 DAZKIF010000000
VERSION     DAZKIF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.159"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(361..624)
                     /gene="rpsT"
                     /locus_tag="ACHOTW_004102"
     CDS             complement(361..624)
                     /gene="rpsT"
                     /locus_tag="ACHOTW_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9846110.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            727..951
                     /locus_tag="ACHOTW_004103"
     CDS             727..951
                     /locus_tag="ACHOTW_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP9846111.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            959..1900
                     /gene="ribF"
                     /locus_tag="ACHOTW_004104"
     CDS             959..1900
                     /gene="ribF"
                     /locus_tag="ACHOTW_004104"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP9846112.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1943..4759
                     /gene="ileS"
                     /locus_tag="ACHOTW_004105"
     CDS             1943..4759
                     /gene="ileS"
                     /locus_tag="ACHOTW_004105"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9846113.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4759..5253
                     /gene="lspA"
                     /locus_tag="ACHOTW_004106"
     CDS             4759..5253
                     /gene="lspA"
                     /locus_tag="ACHOTW_004106"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9846114.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5341..5790
                     /gene="fkpB"
                     /locus_tag="ACHOTW_004107"
     CDS             5341..5790
                     /gene="fkpB"
                     /locus_tag="ACHOTW_004107"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9846115.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5792..6742
                     /gene="ispH"
                     /locus_tag="ACHOTW_004108"
                     /gene_synonym="lytB"
     CDS             5792..6742
                     /gene="ispH"
                     /locus_tag="ACHOTW_004108"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP9846116.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6808..7722
                     /gene="rihC"
                     /locus_tag="ACHOTW_004109"
     CDS             6808..7722
                     /gene="rihC"
                     /locus_tag="ACHOTW_004109"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP9846117.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7889..8710
                     /gene="dapB"
                     /locus_tag="ACHOTW_004110"
     CDS             7889..8710
                     /gene="dapB"
                     /locus_tag="ACHOTW_004110"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9846118.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
BASE COUNT         2000 a         2173 c         2465 g         2083 t
ORIGIN      
        1 aacctgtgtt gattgctgtt aataatttac aaggtctaat gaggagatat ttatgaatgg
       61 ttaaagtata taggaattta attcaaaaat agagtggtct taagtttaat acctcaatgt
      121 gtatcacaat atccacattc tttgtagggg agtctggaga ttgagtagat attcttgttc
      181 agaatgtatc agccgatggt tctacgattc ttaagccacg aagagttcag atagtgcaac
      241 ggcatgtctc ttttgactat ctggcaaccg gcagtgtgtt ctctcacgca tcacaaaagc
      301 agcaggcata aaaaaacccg cttgcgcggg ctttttcaca aagcttcagc aaattggcaa
      361 ttaagccagt ttgttgatct gtgcagtcag gttagcctta tgacgtgcag ctttgttttt
      421 gtggatcaga cctttagcag cctgacggtc cacgatcggt tgcatttcgt taaatgcttt
      481 ctgtgcagca gctttgtcgc cagcttcgat agctgcgtat actttcttga tgaaagtacg
      541 catcatagag cgacggcttg cgttgtgctt acgagccttt tcagactgaa tggcgcgctt
      601 cttagctgat ttgatattag ccaaggtcca actcccaaat gtgttctata tggacaattc
      661 aaaggccgag gaatatgccc ttttagcctt cttttgtcaa tggatttgtg caaataagcg
      721 ccgttaatgt gccggcactc gttacgtagt gatggcgcag gattctacca gcttgcgggg
      781 tgtgaataca gcttttccgc gataaaaatt gcagcaggca gtcagtttct tcccgtgatt
      841 tgcgccatga ccatgacaat gaaaagccac ttctttctga tttcggtact caatcgccgg
      901 ttaaccttga ccgctgtaca aggtatactc ggacgatttt cactgttttg agccagacat
      961 gaagctgata cgcggcatac ataatctcag ccaggccccg caagaagggt gtgtgctgac
     1021 tattggtaat ttcgacggcg tgcatcgcgg tcatcgcgcg ctgttacagg gcttgcagga
     1081 agaagggcgc aagcgcaact taccggtgat ggtgatgctt tttgaacctc aaccactgga
     1141 actgtttgct accgataaag ccccggcacg gctgacccga ctgcgggaaa aactacgtta
     1201 ccttgcagag tgtggcgttg attacgtgct gtgcgtacgt ttcgacaggc gtttcgcggc
     1261 gttaaccgcg caaaatttca tcagcgatct tctggtgaag catttgcggg taaaattcct
     1321 cgccgtgggg gatgatttcc gctttggcgc tggtcgggaa ggcgatttct tgttattaca
     1381 gaaagctggc atggaatacg gcttcgatat caccagtacg caaacttttt gcgaaggtgg
     1441 cgtgcgtatc agcagcaccg ccgtgcgtca ggcccttgcg gatgacaatt tggctctggc
     1501 agaaagttta ctggggcacc cgtttgccat ctccgggcgt gtagtccacg gtgatgaatt
     1561 agggcgcact ataggtttcc cgacggcgaa tgtaccgcta cgccgtcagg tttccccggt
     1621 gaaaggggtt tatgcggtag aagtgctggg cctcggtgaa aagccgttac ccggcgtggc
     1681 aaacatcgga acacgcccaa cggttgccgg tattcgccag cagctggaag tgcatttgtt
     1741 agatgttgca atggaccttt acggtcgcca tatacaagta gtgctgcgta aaaaaatacg
     1801 caatgagcag cgatttgctt cgctggacga actgaaagcg cagattgcgc gtgatgaatt
     1861 aaccgcccgc gaattttttg ggctaacaaa accggcttaa gcctgttatg taatcaaacc
     1921 gaaacacgga accgagaatc tgatgagtga ctataaatca accctgaatt tgccggaaac
     1981 agggttcccg atgcgtggcg atctcgccaa gcgcgaaccg ggaatgctgg cgcgttggac
     2041 tgatgatgat ctgtacggca tcattcgtgc ggctaaaaaa ggcaaaaaaa ccttcattct
     2101 gcatgatggc cctccttatg cgaatggcag cattcatatt ggtcactcgg ttaacaagat
     2161 tctgaaagac attatcgtga agtccaaagg gctttccggt tatgactcgc cgtatgtgcc
     2221 tggctgggac tgccacggtc tgccgatcga gctgaaagta gagcaagaat acggtaagcc
     2281 ggatgagaaa ttcaccgccg ccgagttccg cgccaagtgc cgcgaatacg cggcgaccca
     2341 ggttgacggt caacgcaaag actttatccg tctgggcgtg ctgggcgact ggtcgcaccc
     2401 gtacctgacc atggacttca aaactgaagc caatatcatc cgcgcgctgg gcaaaatcat
     2461 cggcaatggt cacctgcaca aaggcgcgaa gccggtgcac tggtgcgtag actgccgttc
     2521 tgcactggca gaagcggaag ttgagtatta cgacaaaact tctccgtcca ttgacgtcgc
     2581 tttccaggcg gtcgatcagg atgcgctgaa agcgaaattt ggcgtaagca acgttaacgg
     2641 cccaatctcg ctggtgatct ggaccactac gccgtggact ctgcctgcga accgcgcaat
     2701 ctctattgca cctgatttcg actatgcgct ggtgcagatc gacggtcagg ccgtgattct
     2761 ggcgaaagat ctggttgaaa gcgtaatgca gcgtatcggc gtgaccgatt acaccattct
     2821 cggcacggta aaaggtgcgg agcttgagct gctgcgcttt gcccatccgt ttatgggctt
     2881 cgacgtcccg gcaatcctcg gcgatcacgt taccctggat gccggtaccg gtgccgttca
     2941 caccgcgcct ggccacggcc cggacgacta tgtgatcggt cagaaatacg gcctggaaac
     3001 cgctaacccg gttggcccgg acggcactta tctgccgggc acttatccga cgctggatgg
     3061 cgtgaacgtc ttcaaagcga acgacatcgt cgttgcgctg ctgcaggaaa aaggcgcgct
     3121 gctgcacgtt gagaaaatgc agcacagcta tccgtgctgc tggcgtcaca aaacgccgat
     3181 catcttccgc gcaacgccgc agtggttcgt cagtatggat cagaaaggtc tgcgcgcgca
     3241 gtctctgaaa gagatcaaag gtgtgcagtg gatcccggac tggggccagg cgcgtatcga
     3301 gtcgatggtc gctaaccgtc ctgactggtg tatctcccgt cagcgtacct ggggcgtacc
     3361 gatgtctctg ttcgtgcaca aagacacgga agagctgcat ccgcgtaccc tcgaactgat
     3421 ggaagaagtg gctaaacgcg ttgaagttga tggcatccag gcgtggtggg atcttgatgc
     3481 gaaagagatc ctcggcgatg aagctgatca gtatgtgaaa gtgccggata cgctggatgt
     3541 atggtttgac tccggctcta ctcactcttc tgttgttgac gtgcgcccgg aatttgccgg
     3601 tcacgctgcg gacatgtatc tggaaggttc tgaccaacac cgtggctggt tcatgtcttc
     3661 tctgatgatc tccaccgcga tgaagggcaa agcgccgtat cgtcaggtgc tgacccacgg
     3721 ctttaccgta gatggtcagg gccgcaagat gtctaaatcc atcggcaaca ccgtgtcgcc
     3781 gcaggatgtg atgaacaaac tgggcgcgga tattctgcgt ctgtgggtgg catcaaccga
     3841 ctacaccggt gaaatggccg tttctgacga gatcctgaaa cgtgctgccg acagctatcg
     3901 tcgtatccgt aacaccgcgc gcttcctgct ggcaaacctg aacggttttg atccggcaaa
     3961 agatatggtg aaaccggaag agatggtagt actggatcgc tgggccgtag gttgtgcgaa
     4021 agcggcacag gaagacatcc tcaaggcata cgaagcatac gatttccacg aagtggtaca
     4081 gcgtctgatg cgcttctgct ccgttgagat gggttccttc tacctcgaca tcatcaaaga
     4141 ccgtcagtac accgccaaag cggacagcgt ggcgcgtcgt agctgccaga ctgcgctgta
     4201 tcacatcgca gaagcgctgg ttcgctggat ggcaccaatc ctctccttca ccgctgatga
     4261 agtgtggggc tacctgccgg gcgaacgtga aaaatacgtc ttcaccggcg agtggtacga
     4321 aggtctgttt ggtctggcag acagtgaagc aatgaacgat gcgttctggg acgagctgtt
     4381 gaaagtgcgt ggcgaagtga acaaagtcat tgagcaggcg cgtgccgaca agaaagtggg
     4441 tggctcgctg gaagcggcag taaccttgta tacagaaccg gaactggcgg cgaaactgac
     4501 cgcattgggc gatgaattac gatttgtcct gttgacctcc ggcgctaccg ttgcagacta
     4561 taacgacgca cctgctgatg ctcagcagag cgaagtgctc aaagggctga aagtcgcgtt
     4621 gagtaaagcc gaaggtgaga agtgcccacg ctgctggcac tacacccagg atgtcggcaa
     4681 ggtggcggaa cacgcagaaa tctgcggccg ctgtgtcagc aacgtcgccg gtgacggtga
     4741 aaaacgtaag tttgcctgat gagtcaatcg atctgttcaa cagggctacg ctggctgtgg
     4801 ctggtggtag tcgtgctgat tatcgatctg ggcagcaaat acctgatcct ccagaacttt
     4861 gctctggggg atacggtccc gctgttcccg tcgcttaatc tgcattatgc gcgtaactat
     4921 ggcgcggcgt ttagtttcct tgccgatagc ggcggctggc agcgttggtt ctttgccggt
     4981 attgcgattg gtattagcgt taccctggtt gtgatgatgt atcgctcgaa ggccacgcag
     5041 aagctaaaca atatcgccta cgcgctgatt attggcggcg cgctgggcaa cctgttcgac
     5101 cgcctgtggc acggcttcgt tgtcgatatg atcgacttct acgtcggcga ctggcacttc
     5161 gccaccttca acctcgccga tactgccatc tgtgttggtg cggcactgat tgtgctggaa
     5221 ggttttttgc cttctaaagc gaaaaaacaa taataaaccc tgccggataa ggcctacaga
     5281 ttgctgcgaa atcgtaggcc gcatccggca aaaatcctca aaatataaga gcaaacctgc
     5341 atgtctgaat ctgtacagag caatagcgcc gtcctggtgc acttcacgct aaaactcgac
     5401 gatggcacca ccgccgagtc tacccgcaac aacggtaaac cggcgctgtt ccgcctgggc
     5461 gatgcttctc tttccgaagg gctggagcaa cacctgctgg ggctgaaagt gggcgataaa
     5521 accactttct cgctggagcc agatgcggcg tttggcgtgc cgtcaccgga cctgattcag
     5581 tacttctccc gccgtgagtt tatggatgca ggcgagccag aaattggcgc aataatgctt
     5641 tttaccgcaa tggatggcag tgagatgcct ggcgtgatcc gcgaaattaa cggcgactcc
     5701 attaccgtcg atttcaacca tccgctggcc gggcagaccg ttcattttga tattgaagtg
     5761 ctggaaatcg atccggcact ggaggcgtaa catgcagatc ctgttggcca acccacgtgg
     5821 tttttgtgcc ggggtagacc gcgctatcag cattgttgaa aacgcgcttg ccatttacgg
     5881 cgcaccgata tatgtccgtc acgaagtggt gcataaccgc tacgtggtcg atagcctgcg
     5941 cgagcgtgga gctatcttta ttgagcagat cagcgaagtg ccggacggcg cgatcctgat
     6001 cttctccgca catggtgttt ctcaggcggt acgtaacgaa gcgaaaagcc gtgatttgac
     6061 ggtattcgac gccacctgtc cgctggtgac caaagtgcat atggaagtcg cccgcgccag
     6121 ccgtcgtggc gaagagtcta ttctcatcgg tcacgccggg cacccggaag tggaagggac
     6181 gatggggcag tacagcaacc ctgaaggggg aatgtatctg gtcgaatcgc ctgacgatgt
     6241 gtggaaactg acggtcaaaa acgaagagaa gctctccttt atgacccaaa ccacgctgtc
     6301 ggtagatgac acgtctgatg tgatcgacgc gctgcgtaaa cgcttcccga aaattgtcgg
     6361 tccgcgcaaa gatgacatct gctacgccac gactaaccgt caggaagcgg tacgcgccct
     6421 ggcagaacag gcggaagttg tgttggtggt cggttcgaaa aactcctcca actccaaccg
     6481 tctggcggag ctggcccagc gtatgggcaa acgcgcgttt ttgattgacg atgcgaaaga
     6541 tatccaggaa gagtgggtga aagaggttaa atgcgtcggc gtgactgcgg gcgcatcggc
     6601 tccggatatt ctggtgcaga atgtggtggc acgtttgcag cagctgggtg gtggtgaagc
     6661 cattccgctg gaaggccgtg aagaaaatat tgttttcgaa gtgccgaaag agctgcgtgt
     6721 cgatattcgt gaagtcgatt aagtcattag cagcctaagt tatgcgaaaa tgccggtctt
     6781 gttatcggca ttttttatgg agaaaacatg cgcttaccta tcttcctcga tactgacccc
     6841 ggcattgacg atgccgtcgc cattgccgcc gcgatttttg cccccgaact cgacctgcaa
     6901 ctgatgacca ccgtcgcggg taatgtctcg gttgagaaaa ctacccgtaa cgccttgcaa
     6961 ctgctgcatt tctggaatgc ggagattccg ctcgcccagg gagccgctgt gccgctggta
     7021 cgcgcaccgc gtgatgcagc atctgtgcac ggcgaatcgg gaatggctgg ctacgacttt
     7081 gttgagcaca accgaaagcc actcgggata ccggcgtttc tggcgattcg ggatgccctg
     7141 atgcgtgcac cagagcccgt taccctggtg gccatcggcc cgttaaccaa tattgcgctg
     7201 ttactttcac aatgcccgga atgtaaaccg tatattcgcc gtctggtgat catgggtggt
     7261 tctgccggac gcggcaactg tacgccaaac gccgagttta atattgctgc cgatccagaa
     7321 gctgctgcct gtgtcttccg cagtggtatt gaaatcgtca tgtgcggttt ggatgtcacc
     7381 aatcaggcaa tattaactcc tgactatctc tctacactgc cgcagttaaa ccgtaccggg
     7441 aaaatgcttc acgccctgtt tagccactac cgtagcggca gtatgcaaag cggcttgcga
     7501 atgcacgatc tctgcgccat cgcctggctg gtgcgcccgg acctgttcac tctcaaaccc
     7561 tgttttgtgg cagtggaaac tcaaggcgaa tttacctcag gcacgacggt ggttgatatc
     7621 gacggttgcc tgggcaaacc agccaatgta caggtggcat tggatctgaa tgtaaaaggc
     7681 ttccaacagt gggtggctga ggtgctggct ctggtgccgt aacctgtcac atgttatcgg
     7741 catgcagtca ttcatcgact catgcctttc actgatatcc ctccctgttt atcattaatt
     7801 tctaattatc agcgtttttg gctggcggcg tagcgatgcg ctggttactc tgaaaacggt
     7861 ctatgcaaat taacaaaaga gaatagctat gcatgatgca aacatccgcg ttgccatcgc
     7921 gggagccggg gggcgtatgg gccgccagtt gattcaggcg gcactggcat tagaaggcgt
     7981 gcagctgggc gcggcgctgg agcgcgaagg atcttcttta ctgggcagcg acgccggtga
     8041 gctggctggt gcagggaaaa caggcgttac cgtgcaaagc agtcttgatg cgataaaaga
     8101 tgattttgat gtgtttatcg attttacccg tccggaaggt acgctgaacc atctcgcttt
     8161 ttgtcgccag catggcaaag ggatggtgat cggcactacg gggtttgacg aagccggaaa
     8221 acaggcaatt cgtgacgccg ccgccgatat tgcgattgtc tttgccgcca attttagcgt
     8281 tggcgttaac gtcatgctta agctgctgga gaaagcagcc aaagtgatgg gtgactacac
     8341 cgatattgaa attattgaag cacatcatag acataaagtt gatgcgccgt caggcaccgc
     8401 actggcaatg ggagaggcga tcgcccacgc ccttgataaa gatctgaaag attgcgcggt
     8461 ctacagtcgt gaaggccata ccggtgaacg tgtgcctggt accattggtt ttgccaccgt
     8521 gcgtgcaggt gacatcgttg gcgaacatac cgcgatgttt gccgatattg gcgagcgtct
     8581 ggagatcacc cataaggcgt ccagccgcat gacatttgct aacggtgcgg taagatcggc
     8641 tttgtggttg agtggtaagg aaaatggtct ttttgatatg cgagatgtgc ttgatctcaa
     8701 taatttgtaa ccacaaaatg t
//
LOCUS       DAZKIF010000159         8585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZKIF010000159 DAZKIF010000000
VERSION     DAZKIF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.160"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(465..1010)
                     /locus_tag="ACHOTW_004111"
     CDS             complement(465..1010)
                     /locus_tag="ACHOTW_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP9846119.1"
                     /translation="MDNSKHSDEAEKLLAELSARKGEGEGEGEGEGEGEGEGEPKSTV
                     SVFYLQPEEVNTLSHQAKRGDGEAGFRLFLYYKLSNYDEEKSSQWLKIAANNGHATAQ
                     YRLYVELQEQGETKEANEWLQKIKLAASNNDSSAIWFLVNCYKNQGNKKEALQWAYKL
                     KASKQPLDADRLIEELTKEQK"
     gene            complement(1079..5293)
                     /locus_tag="ACHOTW_004112"
     CDS             complement(1079..5293)
                     /locus_tag="ACHOTW_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HFP9846120.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVIRMHD
                     ERTHHYHYDSQHRLVFHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLS
                     RKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEV
                     LQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPE
                     RKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDE
                     ESGLYYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSP
                     SDPIYKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEG
                     MPVVLFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGK
                     DNGVFELVPDEKQPGDFHKFTPTGSK"
     gene            complement(5369..7510)
                     /locus_tag="ACHOTW_004113"
     CDS             complement(5369..7510)
                     /locus_tag="ACHOTW_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9846121.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVLYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            complement(7720..8238)
                     /locus_tag="ACHOTW_004114"
     CDS             complement(7720..8238)
                     /locus_tag="ACHOTW_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9846122.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
BASE COUNT         1713 a         2616 c         2087 g         2169 t
ORIGIN      
        1 aaaagcaaac aaaaaacaat aagcatgttg attgaatatt ttaaatacat gttcttttac
       61 aataaaaaaa tgaatggtta tcgaacaagc cagcaataat aaccttatta ttttcataag
      121 ctccatccac taaaatgctg gagatataaa aaatgaacca aacggcttct ttggcccata
      181 cttaggtttg gggtgtagaa tcttagtcat aagatctatg ccgagtcatg tgctggccac
      241 atacttaatg atacaaaaag ctatattttc atcttttctc ttgcgacttt tgatgcatct
      301 gccaagtaca tcgtaatgat aaccggcgac gaagatgcca gttttggttt caacttgcgc
      361 tttgatgagc ttgttgcagt cgtcgtaaac gtaattttgt gtgtaggtgt tgtagcgact
      421 ggtaaagttt ttctactctc catttttctg ttacctgttt aaagctactt ttgttctttc
      481 gttaattctt caattagtcg atctgcatca agcggctgtt tactcgcttt gagtttatat
      541 gcccattgca atgcttcttt tttatttccc tggtttttat aacaattcac caagaaccat
      601 attgctgaac tgtcattatt actggcagcc aatttaattt tttgtaacca ttcattagct
      661 tccttggttt caccttgttc ttgtaactct acatataacc gatactgagc tgtcgcatga
      721 ccattattag ccgctatttt taaccactga ctactttttt cttcatcata attggacaac
      781 ttgtagtaca agaacaacct aaaacccgcc tcgccatcgc ctcgttttgc ctggtgtgat
      841 aaagtattta cttcttctgg ctgcaaataa aagacgctta ctgttgattt tggttcacct
      901 tcaccttcac cttcaccttc accttcacct tcaccttcac cttcaccttt tctagcagaa
      961 agctccgcca ataatttttc cgcctcatca gaatgttttg agttatccat agcagccctc
     1021 actccaaagt tataaagaca taccatgata atcaacaaaa aagtaaatac tttcattact
     1081 atttgcttcc tgtaggtgta aacttatgaa agtcccctgg ttgtttctcg tctggcacca
     1141 attcaaacac cccgttgtcc ttaccgccta ttgttatcct agctgtttgc cccataatag
     1201 catagtttcc atccggccaa atccatatta tttcatcagg agcaatcact gtagcatcaa
     1261 gttcgttagc tagtttctgt gcaaatgaat ttttgccttt tcctgtttcg caggaaaaca
     1321 agactacagg cataccttca tgatatttag gattcgctct tatgtcgcta gctaattgtt
     1381 ttattgtcag atcacttcct tttttgattc tgtcatcact catagatgta ggcgaaccat
     1441 gacctccaac tgtaaaaccc gttggaaatt cccttacatt aagagaacct ttatatatag
     1501 gatctgacgg agaataataa ttcaaatcaa gtgctaaccc caacggatct atatgttcaa
     1561 taggattgag tgggtactga tacaggttcc atcccccctc cagccctatc gggtcctggg
     1621 tgatatatct cccctgcaac ggatcatagt accggttgcg gttgtagtac agccccgact
     1681 cctcatcata ctgctgcccc ggcagacgga gtgactgttg aaggtgctgc gcgctggttt
     1741 cgcccagcag gtttccccac tcgtcatact ccccctgcca cgccgtttcc ccttccgggc
     1801 tgatgagcgc cagcggcagt ccccggtggt cgcagtggta aagatgaagt ttcctctccg
     1861 ggatgtattc cgcctccagc tgcgcggcca tctgctccgc cgtcagcccg cactgcgcaa
     1921 gccactgctg gctttcttca ctcacgctgc cctgccgcag ctcccgctcc agccttccca
     1981 gcatcaccgc cagctccgcc ggtagcgtca cacccgtgtc ctcctgcaac acctccgcca
     2041 ggctacggtg ccgcgccttc gcctgttcac cattctctgt ttcgattctc aggagcggcg
     2101 tgaagcttcc cggctggtat accgtctgga ttcttgtctg ttgggtctgt acagtggtca
     2161 gcctgtcacc gtcccacccg taccaggtct cctccggttt acgcgacagc gacatccacc
     2221 ccgtcaggtc acgctcccgc cgccacaccc gttttcccgt ccggcggccc agcgggtcat
     2281 agaggtaccg gctctccacc tgtggttcgc cgtgctgtat ccgcgtgtgg aacaccaggc
     2341 ggtgctggct gtcgtagtga tagtggtggg tgcgctcgtc gtgcatccgg ataacccctt
     2401 ccgggatgcg gtccgtcttc tccgccagcc tgccgtattc atcgtagcgg tagacatagt
     2461 gcgcatcttc cgcgatgcgg ttatccggcc atgccgtgag ggtgctgtcc gggtgcagtt
     2521 ccgggtccgg cagccggttt cctgccgggt ccgttgcata cgggatatcg atatccaggt
     2581 tcgctgccgt ggtgtggacg cccgtcagcc ttcccgtgtc actgtaacgg tactcccggc
     2641 tctcctgcgg gccgctgatg cggattagct gtccgttgtc gttccagtcg tagtcacggt
     2701 caagctgcgg aaggttcagg tgcctgctcc ggagctggcc gctggtattc caggcggtgg
     2761 caagttcata tgcctccccg ccgaagctgc gggccgtctc acggtgcagc cggtcccgca
     2821 tgtactcgac cagtggcgtt ccgcccagct tcatccccgc aagataaccg ctgccatacg
     2881 tcagccactc taccggcggc agaccgtccg gctcctgacg ggtcgccagc ccctgttcgc
     2941 tgtacgcgtg ccccgtctca tgctcccaca gcatctcccc cgtctccggg ttctccaccg
     3001 tctgccgttc acccgtcagg cggcctttat cgtcatagcc gtagtggacc gacacccggt
     3061 ggccttcact ggtatggctg agggtggtta gccacccgtg ctcatcatac tgccactgct
     3121 ctgccgggtc gccgttcacc gtccggtgcg tgatgcggtc cgacgcatcg tagtgccaga
     3181 gggtgacaag cccctcgtct tcactctgcg tgagttttcc ggtcaggtca tagtggtaac
     3241 gttgcgtccg gccatcaaaa ccgcgctgtt cagtcagcct gtccaccggg tcataccgga
     3301 acgtggactg gctgccgttc tcgttggtca gcacggtgat gcgcccggca gcgtcatacc
     3361 ccatgctgcg cgtcagaccg ccctgcgtgg tgctgacggc ctttccccac gcatcatact
     3421 gtatctcact gcggctgccg tccggggcaa cgatagcggt gaggtcgcct gcggcgctgt
     3481 actcataacg ggtttcacgc ccctgcgcat ccttctgact gaccagttgg ccacgcgggt
     3541 tataactgct gtaagtgctg atgccttctt cccggtgaac ggcgatttgt tgaccgtacc
     3601 ggtcatactc ataccgcgtc gtgtaccccg agcagtccgt aaaggtcagc agctgaccgt
     3661 aacggctcca tgccatctgt tttgtactgc ccgtcgcgtc ctgtatccct gtcggcagct
     3721 cactcgccgg gtcatcatag ctgtaccgcg tcgtctctcc gctgcgcgag gtctccgccg
     3781 ccagccttcc cttctcatca tactcccggc tgctgcgcag cccgtccggg tacgtcactg
     3841 acgtcacctg ccgctggctg ttatagccat accgcaccgt cctgccgtcc ggccccgtca
     3901 ccgctgtcac cgcacccgac gccatatgca ggctgtactc cgtccgccgt cccgccgcat
     3961 ccgtctgtgc cttcagcctc cccgcctcat catactcgct gcgggtgatg ctcccgtccg
     4021 catgttcctt cttcacaaca cgtttcaggc caccctcgcc ttccgtgtac agcacctccc
     4081 gccggttcag gctgtccgtg atggtcacac ggtcctgccc gtactcaaag cggtagtcca
     4141 gcccctccgg gttgaccagc tccgtcaccc ggccggtatc atcataccgg tagcggctct
     4201 ccggcctacc cgcataatgg tgcgccacca tccgcccggc gtgctccgca tcataagcaa
     4261 acccgcgcac ctgcgtcccg ctgcggtcat acaccgcccg cagttctccg ctggccgtgt
     4321 acgtgtagcg cgccagcggc gcggcgggca gttcatccgg gtatgccggg tcgtgtgtca
     4381 gccataccgc ctccagccgg ataccgttat cggcaccgta ttctgtaccg gcgggcagcg
     4441 tgtcggggaa aaccagagaa gaggaagcag aacggggacc ggcaggggaa gataaagacg
     4501 tggcgcgctg tttacggaat acttccgccc gctgtgcctg tgtggtcagc accaggtgaa
     4561 aacggcggcc cgcgccgtcc gtcacccccg tcaccgcgcc cgccacatca ccctccgccg
     4621 cccggtgaaa ggccagcgtc cgcccgaagc catccaccac gcccgtcagc acccggtatg
     4681 cgggcggctc cggcggcagc acctcgtccg cccccggcac ccgctccggc cagttgagta
     4741 tccaccacgg cccctgaagg ctgtttgtcg ccaggtacat atgcggactc aggcgaacat
     4801 cttccggcag cacctgccag agcgcgctta gcggctgcga actgtgctgc tccgccaccc
     4861 cgccccgcgc cagccagaac gactcgctgc ggctgtagct tatctcgccg ggaaacagcg
     4921 gctcaaagtg aatgctcctg ccgccgttgt cgttgagtat caggccttca tcgcgtatct
     4981 gtaagcggat atcgaagggc gctttccagc cgggaccaaa cacccccacc ggcgcgggtg
     5041 ttctggtccg gtagctgctg taggcgcggg aaagaataaa cggcagcggg ccgggcagcg
     5101 caaggtccgt ctcgcccggc agcaccttcg cacccagcac cgggttcacc gggttagcat
     5161 aggtaatccc tcccggacac accgagcagg ccacgcccgt cggcgcacct atcagcaccc
     5221 ctgctgaacc ctgcacaata tccagtccct tgcgggtcat gtcaccctgc cgcgccgccg
     5281 gttttcctcc catgaatcaa ttccttctgc ttttaatatg gtttatctgg tcactgcacc
     5341 agttccgttt tgattttttg cacctgactc aaaattgccg catatccggc attccggggg
     5401 gtaaatcttt cggcacaggc gcatccggta caggttgtgt ggcaccacag ttccagttaa
     5461 tcaccggcgc atcaccgttc acatccgact ccccttccag atgaatgtgc gtaccattaa
     5521 gaaatatgct tccgtccttc gtcagcacca gccttgcctt accacagcag agttcaagat
     5581 gctcaccggc agaataaact gctgtttgtc cggtgttttc cttcatcgtc ttgcccaccg
     5641 agaaggtgac gttattccct acattgacgc tgtagcccat tcccaccatc agtgatttat
     5701 gcagccctac ctgtgaggac tgcaacaacg ccaccgaggt attcataatg ccgcctaccg
     5761 tcgtctgata cgccacaccc accgtcagcg cacgaatgat ccccaccttt tcaacctggt
     5821 ttgatcccac tttgataatc tggttactgc ctaccgtttc cacctggttg acaccaaccg
     5881 tctgtatctg attaacgcca atgttcagga tctggttatt ggtaacggtg atcgcctggt
     5941 tgttacctat tttttcggca tggttgttta tcacatccgt ggtgcggtta ttcagcacct
     6001 cggtgttcat gttcttctgc gcgtggatgt agacctgttc tttccctgtc gcatcgtcaa
     6061 acttcagttc attaaacccg ctgcccttat aggttttcga acgaatggtc atctgcgtct
     6121 tcgtccccgg caggctaccg ggtgtgcggt tttcctggtg gtaggtacgc cccataatga
     6181 tcggctggtc cggatcgccg ttgaggaagt ccacaatgac ctcctgaccc acacgcggta
     6241 tcgccaggtt accaaacccg gtgcctgccc acgcctgtgc cacacggatc cagcatgaac
     6301 tgtcctggtt tgacgggtta tagcggtccc agttaaattt cacccgcacg cggccatgtt
     6361 catcacagaa gatttcctcg cctgccggtc ccgtcacgac ggcgctctgc gggccgtcca
     6421 ccagcggttt cagcaacggc tgtggtcgcc atgttctgtc tgccggtatt accgcaaaat
     6481 ggttatccag cgtggtgcct gaacccctgc gtcccggcac cgcctgcggc tgctcaccgt
     6541 gcagttcact tgccaccacc tgccattccc ggttcaggtt cgcctgcgga tgccccgtca
     6601 gcacaattcg ccgtcccggc catatctccg gcgaacggct tgttccgcgc gccacttctg
     6661 cgttgtttcg ccagccgtcc atctgccagc gggcaaagtt ctgcccgtgg gcgcccttga
     6721 aacgtccggg atagtcatac acttcatact gtgtgcgctg gtaatcctgg tgctggcctt
     6781 cctggtcaaa acgtccggcc cagccggggc gtttaaaggt gtagtctttg gtcaccacgg
     6841 aagaagggcg gatttgtgcg ctgtagcgaa actggctgat gcagagggtg cttacctcgg
     6901 tacgggtgtt cggattccag gggatctcaa aggactccgg cagataaagc acggtatcgc
     6961 acaggaccag gctctggtcg gtactttttt gcgcgtgctc ctcataaaag aagatgcctt
     7021 cctccgccgc catccggcac aggaaatcgt aatcagtctc accgtactgg acacaaaact
     7081 cacgggaagg atgtggctcg ctgaacagcg ggctccactc ggtcaccccg ttttcctgca
     7141 atatcgtgcc gaggatgctt gcgatgtcct cattctggaa gatacggaag ttctggcgca
     7201 gccctgtgcg ccacagcggc gggcacacct tcatgctgta cagcttctgg tttttgtcgt
     7261 tctcccccag ttcaaaccag gtcaccacac ctttcacccg gcgctgtacg tcatcgccct
     7321 gccatatcgt caggtaggcc attttgtcga ggatctgctg gaattcaagg gagagaaact
     7381 gctggctgac cagagagaga gcgagggaaa aaagcgaaga gagtgactgg ttcagatgaa
     7441 aggagaccac cgcaaaagca tccggtggca ggccgtccac ttccagcgtg aaacgtaatc
     7501 cggttgacat agcaacctcc ggggttaagg aaaaacagac agttcgctgt taaagcggct
     7561 gtgacagtac agtgctacgc ttgcgttcag gcacgctacc gccgaagttt ccggcagccg
     7621 ccatctcccg tgaacgtaac cccttactga caacctttct ttctacggcg taatacaaaa
     7681 acagcatccg gcctgatagc cggatgctgt tggcttaact tatgcttcca gcggtgcgcg
     7741 ccagtcgtcg gcaccggagg tgccagattt gacgtgttcc cattcaattt tgcggtaggc
     7801 cagtgaaact tcgagcaact gggtgaactc gcgtttcgcc gggtcctggc aatgcggcat
     7861 atgcagtttc atatcgacaa tcgtcgaatc agtcaggcgg gtagtgaaat aatgctcctg
     7921 tttgccttcc acagaggtac gccaccagtg aagttcagtg gttggcagca tttcgccgga
     7981 ggccagtgcg ttgtacatca gcggaaccgc tttgttcagc gccacggtaa aaatgaaagg
     8041 cttgtgagca cgctgacccg caggctgacc ggactgagga tcggtcggga cagtaacgtt
     8101 atgcaggaat tcctgcacca gcatctcgtc ttcgtgcccc tgcacataga tgttgccgac
     8161 agaatcagcg gtaaatgcac ctgcagtaat atttccctgg gtctgaccag tgatggagat
     8221 ataacacggt gttggcattt gtaaactcct tgttaacgtg gttaattact acttacaatg
     8281 acctgccgtt aatcccagac aggcagatgc agctcacttc acggaaccgc atcatttttc
     8341 agtatttttt acatacaaac cgagctagaa tttacggttg tcgtaaaaac tatttttaaa
     8401 atcattaaat ctcttattca gcaataactt aataatatat atctggatag ccatacccaa
     8461 ctgttcaaag tccgtattgc ctccataaaa tggccaaagc tgaatcatga ataaatattg
     8521 tatttatcct ttctgaatta tcattttgat aatttttgag atattcctta aaaataccca
     8581 cttaa
//
LOCUS       DAZKIF010000160         8577 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZKIF010000160 DAZKIF010000000
VERSION     DAZKIF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.161"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1847
                     /gene="espL4"
                     /locus_tag="ACHOTW_004115"
     CDS             <1..1847
                     /gene="espL4"
                     /locus_tag="ACHOTW_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFP9846123.1"
                     /translation="SDALDKKELNLNSDRKEKEFPRVKLNGQCYFPGRPQNRIVCRHI
                     AAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDF
                     GLVMKAYFDKMESNDLSVMAAILLVDNHALTVRLRIKNTTEGCIHYVVSVYDPNVTND
                     KIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGTV
                     DEITPPLSPATMNLLMAIGQNHQLKQLMIQLQKMPELHRTEMLTAYNSINLPGLYLAI
                     NYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTS
                     ILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADMLKIVLDALSLLIR
                     TCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISASDIV
                     DLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSNE
                     YPGLFSAIQHKQQNVVEMVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELACEL
                     GHRVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1816..3552)
                     /gene="aceK"
                     /locus_tag="ACHOTW_004116"
     CDS             complement(1816..3552)
                     /gene="aceK"
                     /locus_tag="ACHOTW_004116"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP9846124.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3736..5040)
                     /gene="aceA"
                     /locus_tag="ACHOTW_004117"
     CDS             complement(3736..5040)
                     /gene="aceA"
                     /locus_tag="ACHOTW_004117"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP9846125.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRRYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5070..6671)
                     /gene="aceB"
                     /locus_tag="ACHOTW_004118"
     CDS             complement(5070..6671)
                     /gene="aceB"
                     /locus_tag="ACHOTW_004118"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP9846126.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6940..7869)
                     /gene="metA"
                     /locus_tag="ACHOTW_004119"
     CDS             complement(6940..7869)
                     /gene="metA"
                     /locus_tag="ACHOTW_004119"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP9846127.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8026..8469
                     /locus_tag="ACHOTW_004120"
     CDS             8026..8469
                     /locus_tag="ACHOTW_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9846128.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
BASE COUNT         2198 a         2097 c         2105 g         2177 t
ORIGIN      
        1 gctcagacgc tcttgataaa aaagaattaa atctaaactc agatagaaaa gaaaaagaat
       61 tcccaagagt taagcttaat ggtcaatgtt attttccggg gcgcccccaa aaccgtatag
      121 tatgccgaca cattgctgca caatatatta atgatattta tcagaatgtt gactacaaac
      181 cccatcaaga tgattactct tcagctgaaa aatttctcac tcacttcaac aaaaaatgca
      241 aaaaccagac tttggcgttg atttccagcc gtcctgaggg gcgttgcgtt gctgcctgcg
      301 gtgatttcgg gctagttatg aaggcatatt ttgacaagat ggaatcaaat gacctcagtg
      361 ttatggctgc catattattg gtagataacc atgctttgac ggtccggcta agaataaaga
      421 acacaactga aggatgtatc cattacgtgg tttcggttta tgatcctaat gtaactaacg
      481 ataaaataag aatcatgagc gaaagtaaag aggatattaa acactattca ctgatggatt
      541 ttatgaatgt agattatagc ctcctgaaat ggtcaaatga tcatgttatt aaccaatctg
      601 ttgcaataat tccagcactt ccaaaagaac agctattgat gctaaaagga actgtggatg
      661 aaataacccc tccattatca cctgcaacga tgaatttgct aatggcaatt ggtcagaatc
      721 accaacttaa gcaactgatg attcaactcc agaaaatgcc agaactacat agaacagaaa
      781 tgttgactgc ctataatagt attaacctcc ccggtttata tttggctata aattatggta
      841 atgcggatat cgttgagact atttttaatt cattgtcaga gccaggatat gaaggattac
      901 tctcgaaaaa aaatctcatg catattctgg aggcaaaaga taaaaatggt ttttctggat
      961 tatttttagc gatatcacgt aaggataaaa atgttgtaac ctcgattctg aacgccttac
     1021 ctaaactggc cgcaacacat catttagata acgaacaagt gtataaattc ctgagtgcca
     1081 aaaatagtac gtccagccat gttttatacc atgttatggc gaatggtgat gccgacatgc
     1141 tgaaaattgt tttggacgcg ttatctttgt taattcgcac atgtcatttg actaaagaac
     1201 aggtactcga tctcctgaag gcaaaggatt tttatggttg cccaggacta tacctggcga
     1261 tgcaaaatgg acatagcgat atcgtgaaag ttattcttga agcattgccc agcctggccc
     1321 aggaaattaa catttcagct tccgatattg tagatcttct gaccgctaaa agtcttgcgc
     1381 gcgacacggg tttgtttatg gccatgcagc gcggacatat gaacgttatt aatactattt
     1441 ttaacgcatt acccactctg tttaatacgt ttaaattcga taaaaaaaat atgaagcccc
     1501 tcctcctggc aaataattct aatgaatacc caggtttgtt ttcagcgata cagcataaac
     1561 agcaaaacgt tgtagagatg gtttatcttg ctttatctga ccatgcacgc ctgtttggat
     1621 ttaccgctga agatattatg gatttttggc aacacaaagc gccacaaaaa tactctgcct
     1681 ttgagttggc ttgtgaattg ggtcaccggg ttattgctga attaatcttt aatacattaa
     1741 ataagatggc tgaaagcttt ggctttacgg ataaccctcg atacattgcg gagaaaaatt
     1801 atatggaagc tttactcaaa aaagcatctc cccataccgt acgctaaatc tttgcctgcg
     1861 ccgatacgca taaacatctt ccacatgccc ttcacgtatg cggttttgta gtgcgcgcca
     1921 gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac agcggaccaa tacgcgggtc
     1981 ggcgcatagc caatggcgaa actcttccgg gaaaacatcg cccggcgaga cgctgtacca
     2041 cggttcgctg gcaagttcgt cttccgggta gcgcggcggc gggatatcgc ggaaattcac
     2101 ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc acacgcccgt gacgggtgac
     2161 accgaagttt ttaaacagca tgtcgcccgg gaaaatgtta gcagcggcaa gctggcgaat
     2221 ggcattgccg tactcttcaa tggcgtcgcg cagctgctga ccttccactt gttccagcca
     2281 gatattgagc ggcaccatcc gccgctcaat ataaagatgg cgaatcacaa tttgttcgcc
     2341 gagatcggtg attttttccg ctgcttcctg gagcagtaat tccattaatg ccggggaaat
     2401 atgccgcttc tccagcacaa agttttcaaa ctcctgggta tccgccattc ggcccacgcg
     2461 atcgtgctct ttcaccagtt gatagcaggc acgaacgtga gcggcagaca tctctttctg
     2521 cggcgcgaac ttgtctttga tgactttaaa tacacggtca aagcccggca gcgtgaacac
     2581 caacatcacc atgccacgaa tacccggcgc ttcaatgaac tgctcattac agccctgtag
     2641 ataaacgaga tattcgcggt agctttcggt tttggcgtgc ttctggcagc cgatagccat
     2701 atacaattca gcggtggttt tacctggcag aatttcccgt aaccactcga ccagcgctgc
     2761 gggcagcggc gcataaacca taaaataaga acgcgcaaag ccaaaaacaa tgctcgcttc
     2821 ggcggtcgtc gtcagacagg tatcaataaa cagttcgccg tcatccgtct ggtggatcgg
     2881 caataaaaat ggcaatgtgc cggaaggcgt tattagtttg cccaccagcc aggcggcttt
     2941 attacggtaa aacagttcgt tcgccacctg taaatgactt ttggagaggt tttctggccc
     3001 caacgtttcc gtcagatggc gaataatgta atggatgtca cggcttttat tctgccagtg
     3061 cagacgcaac ggcaggtcgc tgataacgcg catcagcaga gattcccagc cgtgatcggg
     3121 gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag cggcgctctg gctgggagct
     3181 aaagataaaa agccgctcgg gagtaagcga gcggtggtca aataaccgac agtacacgga
     3241 gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc ggcaacagcc gggtgtaatg
     3301 ctctttgacg cgcagtaaaa attccgcgtc cgtgctttgg ccgttggtaa tgcagcgcag
     3361 ttgctccacg accagaccaa cgtgatgatc gtaaagatgg atacggtttt tcatcgcctg
     3421 ctggacagca tgccagtcgg cttgttcgaa acgctgctgc gccccggaag tcacttcgag
     3481 gaatcgacca tactgagcgt cgaagccttg caaaatggtt tgagcaatca gtaattccag
     3541 gccacgcggc atttagcgcc ctcatcagga gcagagaatt gccggatgcg gcgtaaacgc
     3601 cttatccagc ctacgttcgg caacggttgt aggcatgata agacgcgcaa gcgtcgcatc
     3661 aggcatcgtg caccgattgc cggatgcggc gtatatgcct tatccggcct acagtcagca
     3721 acgtttgtag gttgcttaga actgcgattc ttcggtggag ccagtcagcg cggtaaccga
     3781 cgacgtgccg ccctgaataa tggtcgtcac tttatcgaag taacctgtac ccacttcctg
     3841 ctggtgagat acgaaggtgt agccatcttt cgcggcggcg aattccggct gctgcacttt
     3901 ctcaacatag tgcttcatac cctcgccctg ggcataggcg tttgccagat caaacatgtt
     3961 gaaccacatg ctgtggatac ctgccagggt gatgaactgg aacttgtagc ccatatccga
     4021 cagctgctgc tggaagctgg caatagtgtt gtcgtcgagg tttttctgcc agttgaacga
     4081 cggcgagcag ttataagcca gcagtttgcc cggatatttc gcgtggatag cttccgcaaa
     4141 gcgacgcgcc agttccagat ccggcgtgga ggtttcacac cataccaggt cagcatatgg
     4201 cgcatacgcc aggccacggc tgatcgcttg ctcaatgccc gcatgagtac ggaagaagcc
     4261 ttcactggta cgctcgccgg taataaattc gctgtcatac gggtcgcaat cggaggtgat
     4321 cagatccgcc gcatcagcat cggtacgtgc aaccagcagg gttggaacgc ccgtcacgtc
     4381 agctgccaga cgcgccgcga ccagtttctg aatagcttcc tgagttggca ccaaaacttt
     4441 gccgcccatg tgaccgcatt tcttcactga cgccagctga tcttcgaagt gaactgccgc
     4501 tgcaccggct tcaatcatcg ctttcatcag ttcaaaggca ttcaggacac cgccaaaacc
     4561 ggcttccgcg tcggcaacga tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc
     4621 aatgcccgcg gaccattgga tctgatccgc acggcggaaa gtgttgttga tccgctccac
     4681 cacagctggc accgagtttg ccggatagag cgactgatcc ggatacatgc tggccgccag
     4741 gttagcgtcc gccgctacct gccatcccga cagatagacc gcttcaatac ccgctttcgc
     4801 ctgttgcagc gcctgaccgc cagtcagtgc accgaggctg ttgatgtagc cttttttcga
     4861 ctcgccatgc agcagacgcc acattttcgc cgcgcccagt tgcgccagcg tgcattcagg
     4921 attgactgaa ccgcgtaatt tcaccacatc ttccgcgctg tatcggcgag taattccttc
     4981 ccaacgcggt tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat
     5041 gtgcagatgc tccatagtta tgtggtggtt tacgctaaca ggcggtagcc tggcagggtc
     5101 aggaaatcaa ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc ggcatcgtca
     5161 aaacgcccct gggagaaacg ttcttcgccc agttcgctgg caatgacttt catctcttcg
     5221 cccagcatct ggcggaacaa ggctttagtt accggtttgc cattgctcaa cgttttttga
     5281 tgatggatcc actgccagat tgaggtacgg gaaatttcag ccgtcgccgc atcttccatc
     5341 aggccataaa tcggcacgca gccgttgccg gagatccatg cttcgatgta ctgcacagcc
     5401 acgcgaatgt tggcgcgcat accttcttcg gtgcgttcac catcacaagg tgccagcagc
     5461 tgatcggcag taatcggcgc gtcttgttcg cgcatcactt caagctgatt tttacgggag
     5521 ccgagaatgt cgttgaatac cgccattgcc gtgtccgcaa ggcctgggtg agcgatccat
     5581 gtgccatcgt gaccgttatt ggcttccagc gatttatccg cttttacttt gttgagcacc
     5641 tggttattgt gctcttcatc tttgctcgga ataaacgccg ccatgccgcc catcgcaaaa
     5701 gcaccgcgtt tatggcaggt tttaatcaac aggcgtgagt aagcattcag gaatggttta
     5761 tccatcgtta ctgcctgtct gtctggcagg acgcgatcgg gatagttttt caacgttttg
     5821 atatagctga agatgtaatc ccaacgaccg cagttcagac caacaatatg gtcacgcagc
     5881 gcgtgaagga tttcatccat ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc
     5941 ttgatggtgc cgcgcggcag attaaagcga tcttctgcat agctgaagac ttcgctccac
     6001 caggccgctt cctgccagga ctgggttttc ggcagataga aatagggacc actgcccttt
     6061 gccaacagtg cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag gctgccgggg
     6121 attgcctcac cacgccaggt gacatgtttt tccggcaagt gcagaccgcg tacccgacaa
     6181 atcaaaaccg ctggattggg cttgagctgg taaattttgc ctgcttcatt ggtgtaactg
     6241 atggtgccgt taaccgcatc acgcaggtta atttgcccgt cgatcacttt gttccagtct
     6301 ggtgccagtg aatcttcgaa atcggccata aagactttca cattggcgtt gagcgcgttg
     6361 atcaccatct tgcgctctac ggatccggta atctctacgc ggcgatcttc taagtccgca
     6421 ggaatcccgc gaattttcca gtcagcatca cgaatggaag ctgtttccga aataaaatca
     6481 ggcaacgttc cgttatcaat atcttgctgc tgctgaatgc gcgctgccag aagtttattg
     6541 cgttgtggcg taaaatgcgt caccagctca gtcagaaatt ctaccgcttc ggcagtaaga
     6601 atttgcttct cctgctcgcc atacggcctt atgaaagcca gttcatcggt tgttgttgcc
     6661 tgttcagtca tcgtgcagct cctcgtcatg gatccgaaaa cttccccact gtgaacgaag
     6721 gatcgttgtg cgcttttcgt tcagcacaac taagactact catttaaaaa tcaaaatcaa
     6781 aaacaatttc cattttaatt aaaaatactt gataacttat tgataacaat attaataaaa
     6841 tttattcacc tactgaggtg tgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt
     6901 aaggtgctga atcgcttaac gatcgactat cacagaagat taatccagcg ttggattcat
     6961 gtgccgtaga tcgtatggcg tgatctggta gacgtaatag ttgagccagt tggtaaacag
     7021 caaattaccg tgactacgcc agctcgctcg cggtttgttt tgcggatcat tgtgcgggaa
     7081 atagttatac ggtacttccg ggtctagtcc ggcttccaca tcgcggaaat attcctgcgc
     7141 cagcgtttgc gcatcatatt cgggatggcc cgtcacaaag gcaatgcgct tatctttact
     7201 ggcaaacaga tatgcatccc cttcttccgt ctctgccaga atttccagat cggtgtaatc
     7261 acgaatcaac gctgccggaa agtcagcata gcgcgaatgc ggtgccaaga atgaatcatc
     7321 aaagccacgc gtcagaagcg catgaggatg gagaatatga tgctcgtaaa cgccagagag
     7381 tttgtcggtg cgagtttgct taggaatgcc gtagaggata ttgagcgctg cctgtaccgc
     7441 ccagcagaca aacagcgtcg aggtgacgtg atctttcgac cactccagca cctgtttgat
     7501 ctgcggccag taagcgacat cattaaactc caccaggccc agcggcgcac cggttacaat
     7561 cagaccgtca aagttctgtt cctgaatatc ttcaaagtta cagtagaagt tgttcagatg
     7621 ctctgcgggc gtgttacgcg attcacggga atcgatgcgc aacagctgaa tatcgacctg
     7681 caaaggtgag tttgaaagca ggcgcagaaa ctgattttca gtttcaatct tcttcggcat
     7741 caggttaagg atcagaacct taagtggacg aatttcctga ccagacgcac gagaagttgt
     7801 catcacaaag acgttttctt cacgcaagaa attgacggcg ggtagctcgt ccggcacacg
     7861 aatcggcata acctgattac ctcactacat acgcttatac gtttagacat ccagatagct
     7921 gaagataacc agaaaatttg ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa
     7981 ggaaaagact acgttagaat ataagaatga ctaaaaggag agaaaatggt tattagtatt
     8041 cgccgctcac ggcatgagga aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat
     8101 gccacccacg attttttatc agcagagtat cgggccgagc tggaagagct ggttcgttcc
     8161 tttctgccgg aagcaccgtt gtgggtcgcg gttaatgaac gggatcagcc ggttgggttt
     8221 atgttgttaa gtgggcagca tatggatgcg ctgtttatcg atcctgatgt gcgcggctgc
     8281 ggcgtgggcc ggatgctggt gaagcatgcg ctctcaatgg ccccggaact gacaaccaat
     8341 gttaatgagc aaaatgagca ggcggttggg ttctataaga aggtgggttt taaggttacg
     8401 ggacgctctg aggtggacga tttggggaaa ccgtatccgt tgctggatct ggcgtatgtg
     8461 ggggaataag ctgcccccta tcgtgcctgc gtaagctgga taaaacgcgc cagcgtcgca
     8521 tccggcattt ttttgcacca acgcaaaaag gccatccgtc aggatggcct ttcgctt
//
LOCUS       DAZKIF010000161         8558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZKIF010000161 DAZKIF010000000
VERSION     DAZKIF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.162"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(72..353)
                     /locus_tag="ACHOTW_004121"
     CDS             complement(72..353)
                     /locus_tag="ACHOTW_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HFP9846129.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            complement(615..1724)
                     /gene="apbC"
                     /locus_tag="ACHOTW_004122"
     CDS             complement(615..1724)
                     /gene="apbC"
                     /locus_tag="ACHOTW_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP9846130.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1856..3889
                     /gene="metG"
                     /locus_tag="ACHOTW_004123"
     CDS             1856..3889
                     /gene="metG"
                     /locus_tag="ACHOTW_004123"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9846131.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4030..7839
                     /locus_tag="ACHOTW_004124"
                     /pseudo
     CDS             4030..7839
                     /locus_tag="ACHOTW_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7852..>8558
                     /locus_tag="ACHOTW_004125"
     CDS             7852..>8558
                     /locus_tag="ACHOTW_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFP9846132.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     TAAAEKAALKLIAEKVKKGYVEQVDANAHSHDASAKKVLVTEEKASINRQAATNGLPW
                     LADNDPIILPPNIARHALSHRLWPGEPVKKPTKPEKQNLISRLANRTYHYYKNLTFDY
                     SSCSDEWADAISQAISLIKAQKTEIPARTMAVLTFLEICFCQGWNDETSSELLDEIVS
                     TFGIEYATETVIFWWQI"
BASE COUNT         2289 a         2135 c         2063 g         2071 t
ORIGIN      
        1 aataaaaaag ccaaaatcat tcataggaat gattttggct ttttaaatat gcttaaaacg
       61 gtgttggcaa attaatcgta gcgatttaac acgattttgc gtttgtcgtt attacccaca
      121 gctaccactt ctgtcgtaac gccttttgca gtagaagaag cacgatagtt ggtttcaact
      181 cgttgtaaca actgcccaca acgttgctga agatgattat taacaacatc catactgcat
      241 gtaggggaac taattctgcc attgatggtt aatgttgtag tatcagaaga gaacgcgggt
      301 gatgctagtc cataagcaag aaaaacagct cctgacaacc aatacttatt cattgaacgt
      361 tatccctgta gtaaaggtta tgccgggtag attgagtgca taacacacta tagctgtaca
      421 tccgctacac agccacgagg gatgataaag agacagtggt tgcacaatca atcgcctttg
      481 tacagtttcg gactaagatc cactgaatat tcgcagtatt aaataagtgt tcattaatgc
      541 ggcaaaaaca aatacaccgc atcaatataa catctctgcg gtgtatgaag aataaccaga
      601 atggttaata ggcgttagac cgcgcggaag gaaatctcgc ctggaatgac ttcaccctgc
      661 cagtagagct gagctgcaac gcggtcagcc agctggcggt agatagcggt aaattcgctc
      721 tccggacggc tgataaccgt tggcgtgcct ttatcgagat cctcacgtaa ggagatgtgt
      781 aacggcatct gccccagcag ctgtgtatgg tatttttcag ccagtttctc tgcaccaccg
      841 gtaccgaaaa tcggttcatg gtgaccacag ttactgcaaa tatgcacact catgttctcg
      901 acaataccga gcaccggaac ctcgactttc tcgaacatca caatgccttt tttcgcatcg
      961 atcaacgcga tgtcctgcgg ggtggtgacc acgactgcac cagttactgg aatgttttgc
     1021 gccagcgtca attgaatgtc accggtgcct ggcggcatat caagcaccag atagtcgaga
     1081 tccggccaca aggtttcctg caacatctgc attaacgcct tgctcgccat cggtccacgc
     1141 cacaccatcg cattgtcgtc agtgaccaga taaccaattg aattggttgc caggccatga
     1201 gacataatcg gtgccatatg ggtaccgtcc ggcgaagttg gacgctgatt ttctgcgccc
     1261 agcatggtgg gaattgatgg gccgtagata tcggcgtcca gaatgccgac tttcgcccct
     1321 tccgccgcca gtgccagcgc caggtttacc gccgtggagg atttacccac gccgccttta
     1381 ccggaactga ccgcaatgat gttcttcacg ccattaatac ccggctgatt cttcacgcgt
     1441 tttaaggtgg cgatattgtg cgacagtttc cagtcgatag ctttcgcccc ggtgatacgc
     1501 agcagttcgg cgctacattg ctcttttaac tcttcaaacg cgctatgcca gacgaatggc
     1561 ataaccagtt ccacatgcaa cgtgtcgtcc atccaggcga catggtgcaa cgctttcagc
     1621 gtggtgaggt tatgcttcag ggttgggtgc tgaaaattgg ccagtgtccc ggcgaccatt
     1681 gcgcgcaagg cttctggtga tttggcctgg gattgttcgt tcatcccgac tccttttttg
     1741 tatagataaa ccatcagctg atagtttacc tgaagaatat agagaagtac ttacttaaca
     1801 ttttcccatt tggtactatc taaccccttt tcactattaa gaagtaatgc ctactatgac
     1861 tcaagtcgcg aagaaaattc tggtgacgtg cgcactgccg tacgctaacg gctcaatcca
     1921 cctcggccat atgctggagc acatccaggc tgatgtctgg gtccgttacc agcgaatgcg
     1981 cggccacgag gtcaacttca tctgcgccga cgatgcccac ggtacaccga tcatgctgaa
     2041 agctcagcag cttggtatca ccccggagca gatgattggc gaaatgagtc aggagcatca
     2101 gactgatttc gcaggcttta acatcagcta tgacaactat cactcgacgc acagcgaaga
     2161 gaaccgccag ttgtctgagc ttatctatac tcgcttgaaa gaaaacggtt ttattaaaaa
     2221 ccgcaccatc tctcagctgt acgatccgga aaaaggcatg ttcctgccgg accgttttgt
     2281 gaaaggcacc tgcccgaaat gtaaatcccc ggatcaatac ggcgataact gcgaagtctg
     2341 cggcgcgacc tacagcccga ctgaactgat cgagccgaaa tcggtggttt ctggcgctac
     2401 cccggtaatg cgtgattctg aacacttctt ctttgatctg ccctctttca gcgaaatgtt
     2461 gcaggcatgg acccgcagcg gtgcgttgca ggagcaggtg gcaaataaaa tgcaggagtg
     2521 gtttgaatcc ggtctgcaac agtgggatat ctcccgcgac gcgccgtact tcggttttga
     2581 aattccgaac gcgccgggta aatatttcta cgtctggctg gatgcgccga ttggctatat
     2641 gggttctttc aagaatctgt gcgacaagcg cggcgacagc gtaagcttcg atgaatactg
     2701 gaagaaagac tccaccgccg agctgtacca cttcatcggt aaagatattg tttacttcca
     2761 cagtctgttc tggccagcaa tgctggaagg cagcaacttc cgcaagccga ccaacctgtt
     2821 tgttcatggc tatgtgacgg tgaacggcgc gaagatgtct aagtctcgcg gtacctttat
     2881 taaagccagc acctggctga atcattttga cgccgacagc ctgcgttact actacactgc
     2941 gaaactctct tcgcgcattg atgatatcga tctcaacctg gaagatttcg ttcagcgcgt
     3001 gaatgccgat atcgttaaca aagtggttaa cctggcctct cgtaatgcgg gctttatcaa
     3061 caagcgtttt gacggcgtgc tggcaagcga actggctgac ccgcagttgt acaaaacctt
     3121 cactgatgcc gctgaagtga ttggtgaagc gtgggaaagc cgcgaatttg gtaaagcagt
     3181 gcgcgaaatc atggcgctgg ctgatctggc taaccgctat gtcgatgaac aggctccgtg
     3241 ggtggtggca aaacaggaag gccgcgatgc tgacctgcag gcgatttgct caatgggcat
     3301 caacctgttc cgcgtgctga tgacttacct gaagccggta ctgccgaaac tgaccgagcg
     3361 cgcagaagca tttctcaata cagaactgac ctgggatggt atccagcaac cgctgctggg
     3421 ccacaaagtg aatccgttca aggcgctgta taaccgcatc gatatgaagc aggttgaagc
     3481 actggtggaa gcgtctaaag aagaagtgaa agcagctgcc gcgccagtta ctggcccact
     3541 ggcagatgat ccgattcagg aaaccatcac ctttgacgac ttcgccaaag ttgacctgcg
     3601 cgtggcgctg attgaaaacg cagagtttgt tgaaggttct gacaaactgc tacgcctgac
     3661 gctggatctc ggcggtgaaa aacgcaatgt cttctccggc attcgttctg cttacccaga
     3721 tccgcaggca ctgattggtc gtcacaccat tatggtggct aacctggcac cacgtaaaat
     3781 gcgcttcggt atctctgaag gcatggtgat ggctgccggt cctggcggga aagatatttt
     3841 cctgctaagc ccggatgccg gtgctaaacc gggtcatcag gtgaaataat cccccttcaa
     3901 ggcgctgcat cgacagcgcc ttttctttat aaattcctaa agttgttttc ttacgatttt
     3961 gtctctctct aacccgtata aatactggta gcatctgcat tcaactggat aaaattacag
     4021 ggatgcagaa tgagacactt tatctatcag gacgaaaaat cacataaatt ctgggcggtt
     4081 gagcagcagg gaaacgagct gcatatcaac tggggcaaag tcggcactaa cgggcaaagc
     4141 cagataaaaa gttttgcgga tgttgcagca gcggaaaaag cgaaactgaa gcttatcgcg
     4201 gagaaaacga aaaaaggcta tgtggaaaat gcttcagcaa acgtgcatat cccccccatc
     4261 accaaagcca ctcctgaagt tgaaacttcc cctgagagta aaaaccaacg cccgtggctg
     4321 gctgatgatg cagttattcc ggtaactgac gatattaacc gatttgcttt tcctcatcgc
     4381 tctcgcccca gagagatcaa ttatctgcgc aaagacggtg agatatggaa gcgtattgca
     4441 gataacacca gggcatatga tcccgacaac aattaccgct cttatcctga aaactggcag
     4501 caggcttttg ctgagttaca aaggcgaatt ctggataatc aacagacagg gagtgcgcaa
     4561 tctgatgcag cattgctctg gagtttttgg aactcttact ctgcagatga actggtcgat
     4621 gacttagtca ttcgctgtgg tctggaaagc gccgttgaaa tcgtcctttt tgcccttcaa
     4681 ctcaaatata aaccagtaaa aggcgcagta accaccatca ttccacctaa tcacgaagca
     4741 gaatcgctac ctagttagca tcaacgtcta tgtcatcatc tttcactcgc ttcagaagat
     4801 gagtggcaac gctgtgtaga taaagtactc accgccattc cgacgctatc ccccgcacgg
     4861 caaccttttg cggcgttact gctccctgaa cgcccggata tcgcccatgc tatagatcta
     4921 cgctatgcag accaaaacgt cccggcgatg acctggttaa gactggtgat aaccgatgat
     4981 actgctctga gtacaatgga aaaatacgac ttcccaccgt tgtatcgtga ttttcgtaat
     5041 tttcgcgctt atctggcaat gttgttggca aataatgggg tgcgcggtgt aagccggata
     5101 cttcttgagt ttacggaaga ccattctgac aatcccactt atctcttcga acgcatcagc
     5161 gagactgaaa atcttgtcaa atggctatgg aaaacgaatc acccggatgc gattcaaatt
     5221 ctgatcctcg gtgtaattgg taagaaaaag cacctggaat acttaagcaa ggccagtcaa
     5281 aagcatcccg ctgcggctat tgcggcttat gctactttgc tggcaataca tgaagataaa
     5341 gagtggcgta aagcgcttgt caaactgatt accgccacgc cagagttagt atgcgacgtc
     5401 attccctggg ttaatgctaa agcggcaggc atcttatctg aatgtcgtcc gcagtcagtc
     5461 gctgaagaat gtgaatatgc cactgtggat atgttgccgg agctgcttgt ttcgccgccc
     5521 tggatgacca aagagaaaaa gaaaaatacg cctgtgtttg atcttccagt gcttccagtt
     5581 ccttctgttt ctgatgtcac tccagagatt accaaaaaac tgacccgtac ttaccttgtc
     5641 acccacttcc agcaaatagc ccaacagcag gcgacaaaac aaacattgtt taccgacctg
     5701 ccgccgatca aaaaagcaag ctgggagaaa cacttaatac cgcttacacc ggagcaacaa
     5761 atactgtggc atcttggttt tgaaaaatgg cgggagagcg gagaaaaaat atacgaaaaa
     5821 attccggcgc cacaaagcgc cgttgatgcc ctgctacgct tcgattttcc agcactcaat
     5881 gctgaatttg tgcattatca taacaatgca tacaaaagct ggaatctcat tgccctctgt
     5941 tacctgcctg gtcaacaggc catttctttt cttaaccaga tagtcaaaga agataactac
     6001 tctggtgagg gaaatattct ggcaattttt ggtagcgccg ccatacctgc atttatggcc
     6061 tgcctgcaac gtgatccacg acgtctgtgc ttcttcccct ttttcctcgg tgtcagtgaa
     6121 ctggccttac cgatggcgca acaattacag aaaaaaatgt cttatgaaga tgcgcgaaac
     6181 tggctaactg attatccacg ccatgccgcc gccgggctgc ttcctgtggc cctgggcaaa
     6241 aaaggaaaag atcgtgattg tgcccgccag gcgcttcgtc tgctggtgaa tttaaaccag
     6301 cgagaaacga ttgaggaaat cgcgcaagga tataatcaac ctgatgtttt agctgcgctg
     6361 gcaacattgt tcgatagcga cccactggaa gaatatcccg ctaaaatcgc cccactgccc
     6421 ggcttttatc agttcacctt gtggcgcaga ccacggctta aaagtaacaa cctgcctctg
     6481 tcagatgacg ctatgcgcca cctcggcact atgctgagct ttcctcgcga cattaccgcc
     6541 tacgctgggc tggatatcat tagagaaatc ttcacccgcg aatcactggc tgaatttggc
     6601 tgggatctgt atcccgcctg gacagaagct ggcgcacccg caaaagaaaa ctgggcattt
     6661 acttcgcttg ggattttagg caatgacgac accgcgcgta aattaacccc gcttatccgc
     6721 gcctggcctg gggaatccca gcataaacgg gcggtgtccg ggctggatgt attagctgat
     6781 attggcagcg atgttgcgct aatgctgctt aatggcatcg cgcgaaaaat taaattcaaa
     6841 gcattacagg aacatgcccg cgagaaaatc aacatagttg ctgaaaaccg tgggctgact
     6901 atggctgaac tggaagaccg cctggctcca gatttagggc ttgatagcag tggctcgctg
     6961 atactggatt tcggcccccg caaattcacc gttggttttg acgaaacctt aaaacctgta
     7021 gtgtgcgatg caaacggcaa agtcctgaaa gatttaccta agccaaacca gagcgatgaa
     7081 aaaactcagg caactgacgc ggttaatctc ttcaaacagt tgaaaaaaga tgtacgcgcc
     7141 atagccagcc agcagattga tcgtctggaa caggctatgt gccagcgcag acgctggacg
     7201 gcagagcagt tccgcctgtt tctggtggag catccgctgg tacgtcactt aacccgacgg
     7261 ctgttatggg gggtttataa cgatgaaaac gcccttatca cctgttttcg cgtggcagaa
     7321 gacagcactt acagcgatgc gcaggatgag ttattcacgc tgccagcagg aaacatcggt
     7381 attccgcatg tgctggaaat atcccctgaa tccgccgctg cattcaggca aatttacgct
     7441 gattacgaac tgcttccccc tttccaacag ctcgagcgag gtagctatca ccttgctgat
     7501 aatgaacgta atactcacga actgacacgc tggcaaggac ggctttgcca ggccggacgc
     7561 attgtcgggc tggaacgcag aggctggcaa cgtctggaag aaagcggcag cgtttatgcg
     7621 atgcgcaaaa caacccctca tggcgatctc gaacttgaaa cggaaccttt ttcattaatt
     7681 tatggtgaaa cggggtatgg cgaccagcac ccggttgaaa gcgtaaaaat cacctcgcca
     7741 gatgatcgct acggtaaaca atcctcactc actttctcca tgctcgacga catcaccgcc
     7801 agcgagctga ttaacgacat tgaatcactg tttgattaaa aggatttaac aatgaaaacc
     7861 tatatttatc aggacgaaaa atcacataaa ttctgggcgg tggagcagca gggaaacgag
     7921 ctgcatatta actggggtaa agtcggtacc aacgggcaaa gtcaggtaaa aagctttgca
     7981 gatactgcgg cggcggaaaa agcagcactg aagctgattg cggagaaggt gaagaagggg
     8041 tatgtggagc aagtagacgc taacgctcac tcacatgatg cctcagcaaa gaaagtcctg
     8101 gtcacagagg aaaaagcctc aataaatcgc caggcagcaa ctaatggttt accctggctg
     8161 gcagacaacg atcccattat tcttcccccg aatattgccc gccatgccct cagccaccgc
     8221 ctttggccgg gagagccagt aaaaaaacca accaaacccg agaaacaaaa tttaatttcc
     8281 cgattggcaa atcgtactta tcattattat aaaaatctga ctttcgatta cagctcatgc
     8341 tctgatgaat gggctgatgc gattagccag gcaatcagct taattaaggc ccaaaagact
     8401 gagatacccg cgcgtactat ggcggtactt acttttctgg aaatatgttt ttgtcagggc
     8461 tggaatgatg aaacctcatc cgagttgctg gatgaaattg taagcacctt cggtatcgaa
     8521 tacgcaactg aaactgtcat cttctggtgg caaattga
//
LOCUS       DAZKIF010000162         8545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZKIF010000162 DAZKIF010000000
VERSION     DAZKIF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.163"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..452
                     /locus_tag="ACHOTW_004126"
     CDS             <1..452
                     /locus_tag="ACHOTW_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP9846133.1"
                     /translation="HGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQ
                     KKESEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVVNEDDCKIRRGNAAE
                     LFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS"
     gene            718..2955
                     /gene="nrfB"
                     /locus_tag="ACHOTW_004127"
     CDS             718..2955
                     /gene="nrfB"
                     /locus_tag="ACHOTW_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP9846134.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2942..5914
                     /gene="nfrA"
                     /locus_tag="ACHOTW_004128"
     CDS             2942..5914
                     /gene="nfrA"
                     /locus_tag="ACHOTW_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP9846135.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5915..6805
                     /locus_tag="ACHOTW_004129"
     CDS             5915..6805
                     /locus_tag="ACHOTW_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP9846136.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6988..7749
                     /gene="envY"
                     /locus_tag="ACHOTW_004130"
     CDS             6988..7749
                     /gene="envY"
                     /locus_tag="ACHOTW_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFP9846137.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7795..7871)
                     /locus_tag="ACHOTW_004131"
     tRNA            complement(7795..7871)
                     /locus_tag="ACHOTW_004131"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7835..7837),aa:Arg,seq:tct)
     gene            8114..>8545
                     /gene="fimZ"
                     /locus_tag="ACHOTW_004132"
     CDS             8114..>8545
                     /gene="fimZ"
                     /locus_tag="ACHOTW_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFP9846138.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNS"
BASE COUNT         2149 a         2180 c         2184 g         2032 t
ORIGIN      
        1 gtcacggcag agaagaaatc cgtcttcata ttgtttgcga tgtccctgat gaacttattg
       61 atttcacgtt tgaatggaaa gggctgaaga aattatgcgt ggcagtctcc tttcggtcca
      121 taatagcaga acaaaagaaa gagtcagaaa tgacggtcag atattatatc agttctgctg
      181 atttaaccgc agagaagttc gccacagcaa tccgaaacca ctggcacgtg gagaataagc
      241 tgcactggcg tctggacgtg gtagtgaatg aagacgactg caaaataaga agaggaaatg
      301 cagcagaatt attttcaggg atacggcaca ttgctattaa tattttgacg aatgataagg
      361 tattcaaggc agggttaaga cgtaagatgc gaaaagcagc catggacaga aactatctgg
      421 cgtcagtctt tgcggggagc gggctttcgt aatcttgccc tgcctgctca cctccaggca
      481 cttccactac atcatgacct gatcggtgcc accactcctc ttcactatga cgttaacctc
      541 ccctccttga caccacgctt ttgtctacat tctacgcaaa cggcaacttt tataaccagt
      601 gtaaaaataa cgtgccgcaa tagtcatacc tattaatggt aaaaagctgt cacaattcat
      661 aaaaaacctt aatatacgcc accctaaaca taaccagcgt taatgtaagg tttttgtgtg
      721 gactggcttc ttgatgtttt tgctacctgg ctctatggct taaaagtaat cgcgataacg
      781 ttagcggtca tcatgttcat cagcgggctg gacgattttt ttattgatgt cgtctactgg
      841 gtacgccgca ttaaacgcaa gttgagtgtt tatcgccgct acccgcgaat gagttaccgc
      901 gaactgtata aaccagatga aaaaccgtta gcgattatgg ttccggcgtg gaatgaaacg
      961 ggcgtcatcg gcaatatggc cgagctggcg gcgaccacgc tcgactacga aaactatcat
     1021 atctttgttg gcacctaccc caacgacccg gacactcagc gtgatgttga cgaagtgtgc
     1081 gctcgcttcc cgaatgtgca taaggtagtc tgcgcgcgtc ctggccccac cagcaaagcc
     1141 gactgtctga acaacgtgct ggacgccatc acccagtttg agcgtagcgc caatttcgct
     1201 tttgctggtt ttattctgca tgacgccgaa gatgtgattt caccgatgga attgcgtctg
     1261 ttcaactatc tggtcgagcg taaagatctg attcagatcc cggtgtatcc gtttgaacgc
     1321 gaatggacgc acttcaccag catgacttac attgatgagt tttcagagct gcatggcaaa
     1381 gatgttccgg tgcgtgaagc cctcgccgga caagtgccca gcgcaggcgt cggcacctgt
     1441 ttcagccgcc gcgccgtgac cgctctgtta gctgacggtg acggtattgc tttcgacgtg
     1501 cagagtctga ctgaagatta cgacattggc ttccgcctga aagaaaaagg tatgacggaa
     1561 atttttgtcc gttttccggt ggtggacgaa gccaaagaac gcgagcagcg taaattttta
     1621 cagcacgcac ggacgtcaaa catgatctgc gtgcgcgaat atttccccga taccttttcg
     1681 actgcggttc gacaaaaatc tcgctggatc atcggcattg ttttccaggg ctttaaaacc
     1741 cataaatgga cctccagcct gacgctgaac tactttctct ggcgcgaccg caaaggggca
     1801 atcagtaact ttgtcagctt cctcgcaatg ctggtgatga tccagctttt gctgttgctg
     1861 gcgtatgaaa gtttgtggcc cgatgcctgg catttccttt ctatttttag cggcagcgca
     1921 tggttaatga ccctgctgtg gctaaacttt ggcttgatgg ttaaccgcat cgtgcagcgg
     1981 gtgattttcg tcactggcta ctacggcctg acgcaggggc tactatctgt cctgcgtctt
     2041 ttctggggca acctgattaa ctttatggcc aactggcgcg cgttaaaaca ggtacttcaa
     2101 cacggcgatc cacgtcgcgt cgcgtgggat aaaacaacgc atgacttccc cagcgtcact
     2161 ggcgataccc gctcgttgcg cccgttaggt caaattctgc tggaaaatca ggtcatcact
     2221 gaagaacagc tcgatacagc actgcgtaat cgcgtcgaag gtctacgcct gggcggttca
     2281 atgctgatgc aggggctgat tagcgccgag cagctggcac aggcgctggc agagcaaaac
     2341 ggcgtggcgt gggaatccat cgatgcctgg cagatcccgt cctcgctgat tgccgaaatg
     2401 ccggcctccg tggcgctgca ttatgcggta ctgccgctgc gtctggaaaa tgatgagtta
     2461 attgtcggca gtgaagatgg tattgacccg gtttcgctgg cggccctgac gcgtaaagtc
     2521 ggacgcaaag tgcgttatgt cattgttctg cggggacaaa ttgtcacggg gttacgtcac
     2581 tggtatgcac gccgacgcgg tcacgatccg cgggcaatgt tgtacaatgc ggttcagcat
     2641 cagtggctca cggaacagca ggccggtgaa atctggcggc aatatgtgcc gcatcagttc
     2701 ctgttcgccg aaatactgac cacgctcggt catattaatc gttcagcaat taacgtgttg
     2761 ttattgcgcc atgaacgcag ttctctgccg ctcggcaagt ttttggtcac cgaaggcgtt
     2821 atcagccagg aaacgttgga tcgcgtcctg acaattcaac gcgaattaca agtttcgatg
     2881 caatcactat tactcaaagc aggtttaaac acagaacagg ttgcgcaact ggagtccgaa
     2941 aatgaaggag aataacctta atcgcgtcat cggatggtct ggtttactgc tgacgtcttt
     3001 attgagtacc agcgcactcg cagacaatat cggcaccagc gcagaagagc tggggctgag
     3061 cgattatcgc cattttgtta tttatccccg tctcgataag gcgctgaagg cacagaaaaa
     3121 taacgacgaa gcaaccgcca tccgcgaatt tgaatatata caccagcagg tgccggataa
     3181 tattccgctg actttatacc ttgcggaagc ctatcgccat tttggtcatg atgaccgggc
     3241 acggctgttg cttgaggatc aactgaaacg tcacccagga gatgcccgac ttgagcgcag
     3301 tctggcggct attccggttg aagtgaaaag cattacaact gttgaagaac tgcttgccca
     3361 gcaaaaagcg tgcgatgctg cgccgaccct gcgttgtcgc agtgaagtcg ggcagaatgc
     3421 cctgcggctg gcacagttac ctgtcgccag agcgcaactg aacgacgcga cgtttgctgc
     3481 atcgccggaa ggaaaaacgc tgcgaaccga tctgctgcaa cgggcaatct acctgaaaca
     3541 atggtcccag gcagatacgc tatacaatga agcacgccag cagaacacat taagcgcggc
     3601 agaacgccgt cagtggtttg acgtgcttct tgccgggcag ctggacgatc ggatcctggc
     3661 actgcaatca caggggatct tcaccgatcc tcagtcatat attacttacg cgaccgcgct
     3721 ggcttatcgt ggcgaaaaag cacgcctcca gcattatctc attgaaaata agccgctgtt
     3781 taccacggac gcacaagaga aaagttggct ctatctgtta tctaaataca gcgccaaccc
     3841 cgttcaggca ttggcgaatt atacggtgca gtttgccgat aaccgccagt atgttgttgg
     3901 cgcgacgcta ccggtgctgt taaaagaagg tcagtacgac gcagcgcaaa aactgctcgc
     3961 caccctcccc gccaatgaaa tgcttgagga gcgttatgct gtcagcgtgg cgacccgtaa
     4021 caaggctgaa gctctgcgtc tggcacgctt gctgtatcag caagaatcgg caaatcttac
     4081 ccgcctggat caactaacct ggcaactgat gcagaacgag cagtcacggg aagctgccga
     4141 tttattgctg caacgctatc ctttccaggg cgatgcgcgt atcagccaga ctttaatggc
     4201 gcgactggcg tctctgctgg aaagtcatcc ttacctggca acgccggcga aggtggcgat
     4261 tttatcgaaa cccttaccgc tggcggagca acgtcagtgg caaagtcagt tgccgggtat
     4321 tgcagataat tgcccggcaa tagttcgctt gctgggcgat atgtcgcctt cctacgatgc
     4381 cgccgcctgg aaccgtctgg caaagtgtta tcgggacacg ctacccggtg tggcgttgta
     4441 tgcatggctt caggccgaac aacgacaacc gaacgcctgg caacatcgtg cggtagccta
     4501 tcaggcgtat caggttgagg actacgccac cgcactggcg gccaggcaga aaatcagtct
     4561 tcacgacatg agcaatgagg atctgcttgc tgctgccaat accgcccagg cggcaggaaa
     4621 tggtgcggct cgcgatcgct ggctacaaca ggcagaacaa cgtggactgg gaagcaatgc
     4681 cctctactgg tggctgcatg cgcaacgtta cattcctggt cagccggaac tcgcactgaa
     4741 cgatctcacg cgctcaatca atattgcgcc ttctgccaac gcttacgttg cgcgggcgac
     4801 aatttatcgc caacgtcata atgtcccggc cgcggtgagt gatttgcgcg ccgcgctgga
     4861 actggaaccg aataatagca acacccaggc agcgcttggt tacgccttgt gggatagcgg
     4921 tgatatcgca cagtcgcggg aaatgctcga acaggcgcat aaagggctac cggacgatcc
     4981 ggcactgatc cgacaactgg cctatgtgaa ccagcgtctg gatgacatgc ctgcgacgca
     5041 gcactacgcc cggctggtga ttgatgacat tgataatcag gcgctgataa ccccactgac
     5101 cccagagcaa aatcagcaac gcttcaattt ccgccgtctg catgaggagg tcggtcgccg
     5161 ctggacgttc agtttcgatt cttccatcgg cttgcgttcc ggggcaatga gtaccgctaa
     5221 caataatgtt ggcggcgcag cgccagggaa aagctatcgt agctacgggc aactggaagc
     5281 cgaataccgc atcggacgca atatgctgct ggaaggcgac ctgctctcag tttatagccg
     5341 cttctttgcc gataccggag aaaacggggt gatgatgccg gtgaaaaatc cgatgtccgg
     5401 caccggtctg cgctggaagc cgctgcgcga tcagatcttt ttcctcgccg tcgaacagca
     5461 gttgccgctg aacgggcaaa atggcgcatc cgataccatg ctgcgcgcca gcgcctcatt
     5521 ctttaatggc ggcaaataca gcgacgaatg gcacccgaac ggttcaggct ggtttgccca
     5581 aaacctgtac ctcgatgcgg cgcaatatat ccgccaggat attcaggcgt ggacggcaga
     5641 ttatcgcgtc agctggcatc agaaggtagc taacggacag actattgagc cttacgctca
     5701 cgttcaggac aacggctatc gtgataaagg cactcagggc gcgcagcttg gcggtgtcgg
     5761 ggtccgctgg aatatctgga ccggcgagac gcactacgac gcctggccgc acaaagtcag
     5821 tctcggcgtc gagtatcaac atacctttaa ggcgattaat caacgtaacg gagagcgcaa
     5881 caacgcgttt ctcaccattg gagtgcactg gtaaatgcgt aagttcattt tcgtattgct
     5941 gacactgctt ttggtcagcc ctttttcctt tgcgatgaaa ggtattatct ggcaaccaca
     6001 aaaccgtgat agtcaggtta ccgataccca gtggcagggg ctgatgagtc agttacgttt
     6061 gcaaggcttc gataccctgg ttttgcaatg gacccgttac ggcgatgcat tcacccagcc
     6121 agaacagcgc gcgttattgt ttaagcgggc cgcagctgcg caacaggctg gcctgaagct
     6181 gattgtcggg ctgaacgccg atccggaatt ttttatgcac cagaaacagt catccgcggc
     6241 gctggaaagc tatcttaatc gcctgctggc tgccgatctc cagcaagcca gattatggag
     6301 tgccgtacct ggcgtaacgc cggatggctg gtacttcagc gctgaaattg acgacctgaa
     6361 ctggcgcagc gaagccgccc gtcagccttt gctaacatgg ttaaacaacg cgcagcggct
     6421 gattagcgat gtttcagcaa aaccggttta tatcagtagt tttttcgccg gaaacatgtc
     6481 gccagatggc tatcgccaac tgctggaaca cgttaaagca accggcgtta atgtctgggt
     6541 acaggatggc agcggcgtgg ataaacttac cgctgaacag cgtgaacgtt atctacaggc
     6601 cagcgccgat tgccaaagct ccgccccagc aagcggcatt gtttatgaac tgtttgtcgc
     6661 cggcaaaggc aaaaccttta cagcgaaacc gaaaccggac gcagaaattg cctcgctgtt
     6721 agcgaaacgt tcctcttgcg gtaaagacac tctctatttc tctctgcgct atttgcccgt
     6781 cgcgcacggc attctcgagt attaaatctc ctccaggtaa gtcgggtacg acctggctta
     6841 cctctttcgc tctcgtcaga attccatcaa gattaattgc taaaaagcgt gctaaagaaa
     6901 gaagatcgtt atcggcgaac gggccgatca ctcatgcagg aagcatagat cctttgagcg
     6961 gagtttctga tatctgagga gtgcgaaatg caattgagca gcagtgaacc ttgcgtggtg
     7021 atcctgaccg aaaaagaggt agaggtaagc gtcaataacc atgctacgtt tacccttccg
     7081 aaaaactacc tggccgcctt cgcgtgcaac aataacgtca ttgaactctc aacgttaaat
     7141 cacgtattaa tcacccacat caaccgtaac atcatcaacg attatctgtt gtttttaaat
     7201 aagaacttaa cctgtgtaaa gccctggtcg cggctggcta ccccggttat cgcttgtcat
     7261 agccgtacac cggaagtgtt ccggctagcc gccaaccaca gcaagcagca acccagcaaa
     7321 ccctgcgaag cggagttgac gcgcgcattg ctttttaccg tattgtctaa ctttcttgag
     7381 caatcgcggt ttattgccct actgatgtat atcttacgca gcagcgtccg cgacagcgtc
     7441 tgccgcatta ttcaaagcga tattcagcat tactggaatc tgcgaattgt cgccagttcg
     7501 ctatgtttaa gccccagcct gctcaaaaag aaattaaaaa acgaaaatac cagctatagc
     7561 cagattgtca cagagtgtcg tatgcgttac gccgtacaga tgttattgat ggataacaaa
     7621 aatatcactc aggtggcgca attatgtggc tatagcagca cgtcgtactt tatctctgtt
     7681 tttaaggcgt tttacggcct gacaccgttg aattatctcg ccaaacagcg acaaaaagtg
     7741 atgtggtgaa gggcaaagcg gaaacggata agacgggcat aaataaagaa gaaatggcgc
     7801 gccctgcagg attcgaacct gcggcccacg acttagaagg tcgttgctct atccaactga
     7861 gctaagggcg cgttgatacc gcaatgcggt gtaatcgcgt gaattatacg gtcaaccctt
     7921 gctgagtcaa tggcttttga tctggttgct gaacaagtga acgaccgcgt ctgattttct
     7981 gatttatttc gctatagcgg caaacaaacg cacaccgctg cgcgtctgaa tcaagaaaac
     8041 ccgtattttc atgtatcaaa gtacaattta ccgccctaac ggaaaattgt ccgctcctat
     8101 gagactggta actatgaaac caacgtcggt gatcattatg gatactcatc ctatcatcag
     8161 aatgtctatt gaagttctgt tgcaaaaaaa cagtgaattg cagattgtcc tgaaaacgga
     8221 tgattaccgc ataaccatcg attatctccg aacccgtcct gttgatttaa tcattatgga
     8281 tatagacttg cccggaacag acggttttac cttcctgaaa aggatcaaac aaatccagag
     8341 cacagtgaaa gtgttatttt tatcatcgaa atcagaatgc ttttatgctg gcagagcgat
     8401 acaagctggt gctaacggtt ttgtcagtaa atgcaatgat cagaatgata tttttcatgc
     8461 cgttcagatg atcctctccg gatacacgtt ttttcccagc gaaacgctta actatataaa
     8521 aagcaataaa tgtagtacga atagt
//
LOCUS       DAZKIF010000163         8477 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZKIF010000163 DAZKIF010000000
VERSION     DAZKIF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.164"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(157..363)
                     /locus_tag="ACHOTW_004133"
     CDS             complement(157..363)
                     /locus_tag="ACHOTW_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP9846139.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            676..765
                     /gene="kdpF"
                     /locus_tag="ACHOTW_004134"
     CDS             676..765
                     /gene="kdpF"
                     /locus_tag="ACHOTW_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP9846140.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            765..2438
                     /gene="kdpA"
                     /locus_tag="ACHOTW_004135"
     CDS             765..2438
                     /gene="kdpA"
                     /locus_tag="ACHOTW_004135"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP9846141.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2461..4509
                     /gene="kdpB"
                     /locus_tag="ACHOTW_004136"
     CDS             2461..4509
                     /gene="kdpB"
                     /locus_tag="ACHOTW_004136"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP9846142.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4518..5090
                     /gene="kdpC"
                     /locus_tag="ACHOTW_004137"
     CDS             4518..5090
                     /gene="kdpC"
                     /locus_tag="ACHOTW_004137"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP9846143.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5083..7767
                     /gene="kdpD"
                     /locus_tag="ACHOTW_004138"
     CDS             5083..7767
                     /gene="kdpD"
                     /locus_tag="ACHOTW_004138"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP9846144.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7764..8441
                     /gene="kdpE"
                     /locus_tag="ACHOTW_004139"
     CDS             7764..8441
                     /gene="kdpE"
                     /locus_tag="ACHOTW_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP9846145.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITETG
                     IGYRFML"
BASE COUNT         1793 a         2194 c         2453 g         2037 t
ORIGIN      
        1 tcagatagaa acaattagga aaatatttat atatatttca tgaatatatg tttcgattgg
       61 tataacggtg tctaaatatt cctctgaaca tcttcctgat acaaaaaacc gccagcgttt
      121 ccgctggcgg ttttcaatgt attcaaaact cagcaatcag taaaaatcac cggttgcctt
      181 ttcggcctga tccatccaaa ccggtttatc gctggttttc gaccagacgc gatgcaggta
      241 gctgtaaaag cgggcgcggt ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc
      301 tgcaaccgca taagtacggc gtaaaaagat aagccatgca ggatattctc tgtagagttc
      361 catacttttc tccccctttt gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt
      421 tatctgatga aaatagtacc gccttttgtg taattttact actcatccga ccacttattt
      481 ttgcttattg atggtttatt tacattcacc ctgtaattaa gttacacaaa agttaaatta
      541 atactaaaca ttagttaaat catggctttt gccattttta tacttttttt acaccccgtc
      601 cgcagatttt tgcgaaatct ttgcagccag aattctaccc ttccggtatc acttttaggc
      661 cactggaggt gcactgtgag tgcaggcgtg atagccggcg tgttgctggt gtttttatta
      721 ctgggttatt tggtttatgc cctgatccat gcggaggcat tctgatggct gcgcaagggt
      781 tcttactgat tgccacgttt ttactggtat taatggtgct ggcgcgtccc ttaggtagcg
      841 ggctagcgtg gctgattaat gatattcctc ttcctggtac gacgggcgtt gaacgcgtac
      901 tctttagcgc gcttggcgtc tctaaccatg agatgaactg gaagcagtat ctctgtgcca
      961 ttctcggcct gaacatgctg gggctggcgg tgctgttttt tatgttgctc ggtcagcact
     1021 atctgccgct taatccacag cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg
     1081 ccgtcagctt tgtcacgaat accaactggc aatcttatag cggtgaaacc acgttgagct
     1141 atttcagcca gatggcgggc ttaacggtgc aaaactttct ttctgccgcc agcgggattg
     1201 cggtgatttt tgccctcatt cgtgcattta cccgccagag catgagcaca ctcgggaatg
     1261 cctgggtcga tctgctacgc atcacgttat gggtgctcgt ccctgtggcg ttggtgatcg
     1321 cactgttttt tattcaacaa ggtgcgctgc aaaactttct gccctatcag gcagtgaata
     1381 ccgttgaagg agcgcaacag ctgttaccaa tgggtccggt ggcttctcag gaagcgatca
     1441 agatgctcgg cactaacggc ggtggtttct ttaatgccaa ctcgtcgcat ccgtttgaaa
     1501 acccaaccgc actgaccaac ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc
     1561 tgtgctttgc ctttggtgaa gtgacgggcg atcgccgtca ggggcgcatg ttgctgtggg
     1621 cgatgtcagt gatttttgtc atctgcgtag gcgtggtgat gtgggcagaa gttcagggta
     1681 atcctcatct gctggcgctc ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc
     1741 gtttcggcgt gctggtcagt agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg
     1801 cggtgattgc aatgcatgat tcgtttaccg ctctcggtgg catggtgccg atgtggctga
     1861 tgcaaattgg tgaagtggta ttcggcggtg tcggttctgg tctttacggc atgatgctgt
     1921 ttgtcctgct ggcggtgttt attgccgggc tgatgattgg tcgtacaccg gaatatctgg
     1981 gtaaaaaaat cgacgtacgt gagatgaaac tgactgcact ggcgattctg gtcaccccga
     2041 cgctggtgct gatgggcgcg gcgctggcaa tgatgaccga cgccggacgt agcgccatgc
     2101 tcaaccctgg cccgcatggt tttagcgaag tgctgtacgc cgtgtcatcc gccgctaaca
     2161 acaacggcag cgcctttgcc ggattaagcg ccaactctcc gttctggaac tgtttactgg
     2221 cgttctgcat gtttgtcggt cgcttcgggg tgattatccc ggtgatggca attgccggtt
     2281 cgctggtgag taaaaagagc caacccgcca gctccggcac gctgccaacg cacggcccgc
     2341 tgtttgttgg cctgttaatc ggcaccgtgt tgctggttgg cgcactgacc tttatccctg
     2401 ccctggcgct tggtccggtg gcggaatatc tctcctgatg atattgagtg agcactgaat
     2461 atgagtcgta aacaactggc gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa
     2521 gcggtgaaaa aattaaaccc gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg
     2581 atcggcagtc tgctgaccac ctgtattagc atcgcgatgg caagcggcgc gatgcctggc
     2641 aatgcgctgt ttagcgtggc cattagcggc tggctgtggg tcaccgtact gttcgctaat
     2701 ttcgccgagg cgctggcaga aggccgcagt aaagcgcagg ccaacagcct gaaaggggtg
     2761 aaaaaaacgg cctttgcccg caagctgcgt gagccgaaat atggcgctgc ggcggacaaa
     2821 gttcccgccg atcaactgcg taaaggcgat atcgtactgg tagaagcagg cgatattatc
     2881 ccctgcgatg gtgaagttat tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg
     2941 gaatcggcac cggtgatccg tgaatccggc ggcgattttg cctccgtcac cggcggcacg
     3001 cgtattcttt ctgactggct ggtgattgag tgtagcgtta accccggcga gacatttctg
     3061 gatcggatga tcgcgatggt ggaaggcgca cagcgacgca aaacgccgaa cgagattgcc
     3121 ctgaccatcc tgctgattgc cctgactatc gtctttttac tggcaaccgc cacgctgtgg
     3181 ccgttttccg cgtggggcgg taatgcagtc agcgtaacgg tactggtggc gctgctggtc
     3241 tgtctgatcc caaccactat tggcggcctg ttgtcagcga tcggcgtcgc cgggatgagt
     3301 cggatgctgg gcgcgaatgt ggttgccacc agcggacgtg cagttgaagc ggcaggtgac
     3361 gttgacgttc tgctgctgga taaaaccggc accatcacac tcggtaaccg tcaggcatcg
     3421 gagtttatcc ctgcgcaggg cgtggatgaa aaaacgctgg ctgacgccgc acaactggct
     3481 tcgctggctg atgaaacgcc ggaaggccgc agtattgtga tcctcgccaa gcagcgtttt
     3541 aacctgcgcg agcgcgatgt gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa
     3601 agccggatga gcgggatcaa tatcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc
     3661 attcgccgcc atgttgaggc taacggtggt cacttccctg ccgatgttga tcaaaaagtc
     3721 gatcaggttg cgcgtcaggg ggccacaccg ctggtagtag tagaaggttc tcgtgtgctg
     3781 ggcgttattg cgttgaaaga tatcgtcaaa ggcggcatta aagaacgctt cgcccaactg
     3841 cgcaaaatgg gtattaaaac ggtgatgatt accggcgata accgtctgac tgccgccgcg
     3901 atagctgcgg aagcgggcgt cgatgatttt ctcgccgaag cgacaccgga ggccaagctg
     3961 gcattgatcc gtcagtatca ggcggaaggc cgtttggtag caatgaccgg cgacggcacc
     4021 aacgatgctc cggcgctggc gcaggcagat gtcgcggtgg cgatgaactc cggtactcag
     4081 gcggcgaaag aagcgggcaa tatggtggat ctcgactcta acccgaccaa gttgatcgag
     4141 gtggtacata ttggcaaaca gatgctgatg acccgtggct cgctgaccac cttcagcatt
     4201 gccaacgatg tggcgaaata cttcgccatt attccggcgg cattcgcggt aacgtatcca
     4261 cagttaaatg cgctgaacat catgcgcctg cattcgcccg actccgccat tctcagtgcg
     4321 gtcattttca acgccttgat tatcgtcttt ttgattcccc tggcgttaaa aggcgtgagt
     4381 tataaaccgc ttaccgcttc tgccatgttg cgccgtaact tatggattta cggtctgggt
     4441 gggctgctgg tgccgtttat tggtatcaaa gtcattgatt tactactgac cgtttgcggt
     4501 ctggtgtgag gtttaccatg cgtggattac gtccggcatt atcgacattt atctttctgt
     4561 tattgattac tggcggtgtt tacccgctgc tgaccaccgc gctggggcaa tggtggtttc
     4621 cctggcaggc caacggttcg ttgatccgtg aaggtgatac ggtgcggggt tcggcattaa
     4681 tcgggcagaa ttttaccgac aacggctatt ttcatggtcg cccgtcggca acggcagaaa
     4741 tgccttataa tccacaggct tctggcggga gcaatctggc ggtcagtaac cctgagctgg
     4801 ataaactaat agccgcacgc gttgctgcat tacgggccgc taacccgaat gccagcacga
     4861 gcgttccggt tgaactggtg acggcgtcgg caagcgggct ggacaataac atcaccccgc
     4921 aagcggcggc ctggcagatc ccacgcgtag cgaaagcgcg taatctcagc gttgaacagc
     4981 tcacgcaact gatcgcaaaa tacagccaac aaccactggt gaaatatatc ggccagccgg
     5041 tcgtcaacat tgtcgaactc aatctggcac tagataaact tgatgaataa cgaaccctta
     5101 cgtcccgacc ccgatcgtct gctggaacaa actgccgcgc cgcatcgggg gaagctgaaa
     5161 gttttcttcg gtgcctgcgc aggcgtcggg aagacctggg cgatgctggc agaagcccag
     5221 cgactgcggg cgcaagggct ggatattgtg gttggcgtgg tagaaaccca cgggcggaaa
     5281 gataccgccg ccatgctgga agggctggct gttctgccgc caaaacgcct ggcgtaccgt
     5341 gggcggcaca tcagcgagtt tgatctcgat gccgccctcg cccgccgtcc ggcgctgatc
     5401 ttaatggacg aactggcgca cagtaatgcg ccaggttctc gtcatcctaa acgctggcag
     5461 gatatcgaag aactgctgga agctggcatt gatgttttca caaccgtcaa cgttcagcat
     5521 ctggaaagtc tgaatgatgt ggtcagcggc gtcaccggaa ttcaggtacg ggaaaccgtg
     5581 cccgatcctt ttttcgatgc cgccgacgac gtggtgctgg tggacttgcc cccggacgat
     5641 ctgcgccagc ggctgaaaga aggcaaagtc tatattgccg ggcaggcgga gcgcgccatt
     5701 gaacattttt tccgcaaagg caatctgatc gccctgcgca aactggcact gcgccgtact
     5761 gccgatcgcg ttgatgagca aatgcgcgcc tggcgggggc atcctggcga agagaaagtc
     5821 tggcacacgc gcgacgcgat ccttttatgc atcggccata acaccggcag cgaaaaactg
     5881 gtccgcgcgg cggcgcggct ggcgtcacgg ctggggagcg tctggcacgc ggtgtatgtt
     5941 gaaacccctg ccctgcatcg cttaccggag aaaaaacgtc gggcaattct cagcgcctta
     6001 cgtctggcgc aggagctggg cgcggagacg gcaacacttt ctgatccagc ggaagagaaa
     6061 gcggtagtgc gttatgcccg tgaacataat ctcggcaaga ttattctcgg tcgcccagcc
     6121 tcgcgccgct ggtggcgtcg ggaaacgttt gctgaccgac tggcgcgcat cgcccccgat
     6181 ctcgatcagg tgctggtcgc gcttgatgaa ccacccgccc gcacgattaa caacgcgccg
     6241 gatagccgct cttttaaaga caagtggcgg gtacagattc agggatgcgt ggttgccgcc
     6301 gcgttatgcg ccgttatcac cttaattgcc atgcagtggc tgatggcgtt tgatgccgcc
     6361 aacctggtga tgctgtatct acttggcgtg gtggtggtgg cgctatttta tggacgctgg
     6421 ccttcggtgg ttgctaccgt cattaatgtg gtgagtttcg atctcttttt tatcgcccca
     6481 cgcggcacgc tcgccgtctc tgatgtgcaa tatctgctga ccttcgcggt gatgttaacc
     6541 gtcgggctgg tgatcgggaa ccttactgcg ggtgtgcgtt atcaggcgcg ggtagcccgt
     6601 taccgcgagc aacgcacacg gcacttatat gaaatgtcga aagctctggc ggtgggccgc
     6661 agtccgcagg acatcgccgc caccagcgaa caatttattg cctccacgtt tcatgcccgc
     6721 agtcaggtgt tgttgcccga tgacaacggt aaattgcagc cgttaacaca tccgcaagga
     6781 atgacgccgt gggacgatgc catcgcgcag tggagttttg ataaaggcct gcctgcgggt
     6841 gcgggcaccg acacgttacc cggcgtgccg taccagattt tgccgttaaa aagcggtgag
     6901 aaaacctacg ggctggtggt ggtggaaccg gggaatctgc gtcagttgat gatcccggaa
     6961 cagcagcgcc tgctggagac gtttacgctg ttagtcgcca atgcgcttga gcggctgacg
     7021 ctgaccgcca gcgaagaaca ggcgcggatg gcaagcgaac gtgaacagat ccgcaacgcc
     7081 ctgctggcgg cgctttcgca tgatttacgc actccgctta cggtgctgtt tggtcaggca
     7141 gaaatcttaa cgctcgatct ggcaagcgaa ggatcacccc acgcccgcca ggccagcgag
     7201 attcgtcagc atgtgctgaa cactacccga ctggtgaata atctactgga tatggcgcga
     7261 attcagtccg gcggctttaa tttgaagaaa gagtggttaa cgctggaaga agtagtcggc
     7321 agcgcgctgc aaatgctgga accgggttta tcgtcgccca tcaatctttc tctgccagaa
     7381 ccgctgacct taatccacgt tgacgggcca ctctttgaac gggtgctgat taatctgctg
     7441 gagaacgcgg tgaaatatgc gggtgcgcag gccgaaattg gtatcgatgc ccacgttgag
     7501 ggcgaaaatc tacaactgga tgtctgggat aacggccccg gtcttccgcc aggccaggag
     7561 cagacgatat ttgataagtt tgctcgcggg aataaagagt cggcagtacc gggggtaggg
     7621 cttggactgg caatttgtcg ggcgatagtg gatgtacacg ggggcactat taccgcgttc
     7681 aaccggccgg aaggtggtgc ctgttttcgt gttacacttc cccagcaaac tgcccctgaa
     7741 cttgaagaat ttcatgagga tatgtgacaa acgttctgat tgttgaagat gaacaggcta
     7801 ttcgtcgctt tctgcgcacg gcgctggagg gcgacgggat gcgcgtctat gaggccgaaa
     7861 cgctgcaacg cggcttgctg gaagcggcta cccgtaagcc agatttgatt attctcgatc
     7921 tcggcctgcc cgatggtgat gggattgagt ttatccgcga cctgcgccag tggagcgcgg
     7981 tgccggtgat tgtgctttcc gcacgcagcg aagagagcga caaaatcgcc gcgctggatg
     8041 ccggagcgga tgattatcta agtaagccgt ttggcattgg cgaattgcag gcccgtctgc
     8101 gcgtcgcttt acgccgccac tccgccaccg ccgcgcccga tccgctggtg aaattttccg
     8161 atgttaccgt cgatttagcc gcccgcgtga ttcaccgggg tgaggaagag gtgcatctca
     8221 ccccgattga gtttcgcctg ctggcagtgc tgctcaacaa cgccggaaaa gtgctcaccc
     8281 agcgccaact ccttaaccag gtgtgggggc caaacgcggt cgaacacagt cattatttgc
     8341 gtatttatat gggacatctg cgacaaaaac tggaacagga tcccgctcgc cccagccatt
     8401 tcattactga aaccggtatt ggttatcggt ttatgctttg aatttttatt caataaattc
     8461 gcctaaatag tgattaa
//
LOCUS       DAZKIF010000164         8460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZKIF010000164 DAZKIF010000000
VERSION     DAZKIF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.165"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..579
                     /locus_tag="ACHOTW_004140"
     CDS             <1..579
                     /locus_tag="ACHOTW_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFP9846146.1"
                     /translation="RSYIHPDEDNKFDNFMRQNRFSKVNFDTQQMIDFINRFNMNKWL
                     IDRNNNFFIQLIDQALRSTNDTIKENAWHLYKEWIRSDDVSPLFIEIEDNLRTFNTNE
                     LTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTP
                     ETLFGSKSYDKHALFPIFTASWRANRIKNKGI"
     gene            802..1299
                     /gene="ubiC"
                     /locus_tag="ACHOTW_004141"
     CDS             802..1299
                     /gene="ubiC"
                     /locus_tag="ACHOTW_004141"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP9846147.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1312..2184
                     /gene="ubiA"
                     /locus_tag="ACHOTW_004142"
     CDS             1312..2184
                     /gene="ubiA"
                     /locus_tag="ACHOTW_004142"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP9846148.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2339..4762)
                     /gene="plsB"
                     /locus_tag="ACHOTW_004143"
     CDS             complement(2339..4762)
                     /gene="plsB"
                     /locus_tag="ACHOTW_004143"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP9846149.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4933..5301
                     /gene="dgkA"
                     /locus_tag="ACHOTW_004144"
     CDS             4933..5301
                     /gene="dgkA"
                     /locus_tag="ACHOTW_004144"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9846150.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5411..6019
                     /gene="lexA"
                     /locus_tag="ACHOTW_004145"
     CDS             5411..6019
                     /gene="lexA"
                     /locus_tag="ACHOTW_004145"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9846151.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6092..7417
                     /gene="dinF"
                     /locus_tag="ACHOTW_004146"
     CDS             6092..7417
                     /gene="dinF"
                     /locus_tag="ACHOTW_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP9846152.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7533..7742
                     /gene="yjbJ"
                     /locus_tag="ACHOTW_004147"
     CDS             7533..7742
                     /gene="yjbJ"
                     /locus_tag="ACHOTW_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9846153.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7784..8299)
                     /gene="zur"
                     /locus_tag="ACHOTW_004148"
     CDS             complement(7784..8299)
                     /gene="zur"
                     /locus_tag="ACHOTW_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP9846154.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1950 a         2042 c         2287 g         2181 t
ORIGIN      
        1 cgtagttata tccatcctga tgaagataac aagttcgata attttatgcg tcaaaatcga
       61 ttcagtaagg tgaatttcga tacccaacag atgatcgatt ttattaacag atttaatatg
      121 aataaatggc tgattgatcg aaataacaat ttttttatcc aacttatcga tcaggctcta
      181 cgatcaacga atgatacgat caaagaaaat gcctggcatc tttataaaga gtggattcgt
      241 agtgatgatg tttcaccttt atttatagaa attgaagata atttaagaac ctttaacacg
      301 aatgaattaa cacgaaacga taatatcttt atcttgttct cctctgtcga tgatgggcca
      361 gttatggtgg taagctccca gcgcttacat gatatgttga atcctacaaa agataccaat
      421 tggaattcca cgtatatcta taaatccaga catgagatgt tgcctgttaa tcttactccg
      481 gaaacacttt tcggctccaa atcttatgat aaacatgcgc ttttccccat ttttactgcg
      541 agttggcgag ctaatcgtat aaagaataaa ggtatttaag taaaggaaaa catcaccgct
      601 cttggcatca tgggcggtga tgcccctatg gttgccctcg ccagcacggg catcggcaaa
      661 gcgtaaggtt caacagcgtt ttaccacttc atgcgattgt tgcgtttttg ttgcgcatta
      721 gatcacttaa tttgctttac atctcccgta aacacttttc tgcgatacaa tgcctttacg
      781 ttatgtaacg gagagttcgg catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc
      841 tattgtaaag agatccctgc cctggagccg caactgctcg actggctgtt gctggaagat
      901 tccatgacaa aacgttttga acagcaggga aaaacggtga gcgtgacgat gatccgcgaa
      961 gggtttgtcg agcagaatga aatccccgaa gaactgccgc tgctgccgaa agagtctcgt
     1021 tactggttac gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc
     1081 gtcgttcctg tgtcaacgtt aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg
     1141 ccgttaggac gctatctgtt cacatcatcg acattaaccc gggactttat tgagataggc
     1201 cgtgatgccg gactgtgggg gcgacgttcc cgcttgcgat taagcggtaa accgctgttg
     1261 ctaacagaac tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg
     1321 agtctgacgc agaataagct gctggcgttt catcgcttaa tgcgtacgga taagccaatt
     1381 ggcgcgttac tgctgctctg gccaacatta tgggcgttgt gggtggcgac accgggcgtt
     1441 ccccagctct ggatcctggc ggtgtttgtc gcgggtgtct ggctgatgcg cgctgccgga
     1501 tgtgtggtga atgattatgc tgaccgcaag tttgatggtc atgttaagcg cacggcgaac
     1561 cgaccacttc ccagcggcgc ggtaacagag aaagaggcgc gcgcgctgtt tgtagtgctg
     1621 gtactgattt cgtttttact ggtgctgacg ctgaatacga tgaccattct gttgtcgatt
     1681 gccgcgctag cgctggcgtg ggtgtacccg tttatgaagc ggtataccca tctaccgcaa
     1741 gtggtgctgg gcgcggcgtt tggctggtcg attccaatgg cttttgctgc tgtgagtgag
     1801 tcggtgccat tgagttgctg gttaatgttc ctcgccaata ttctctgggc ggtggcttac
     1861 gacacgcagt atgcgatggt tgaccgtgat gatgatgtga agattggcat taaatccacc
     1921 gcaatcctgt tcggccaata cgataaattg attattggta ttttgcagat tggcgtactg
     1981 gcgctgatgg cgattatcgg tgagttaaat ggcttaggct ggggatatta ctggtcaatt
     2041 ctggtggctg gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg cgagcgcgaa
     2101 gcctgcttta aagcatttat gaataataac tatgttggtc tggtactgtt tttagggctg
     2161 gcaatgagtt actggcattt ctgatgatgt aaaaaagccg gatgatcatc cggctttctt
     2221 ctgggttgcc tgatgcgctg cgcttctcag gcctacacaa cagatcgcaa tttattgaat
     2281 ttgcagatta tggaaggccg gataaggcgt ttgcgccgca tccggcaatt ctctctgatt
     2341 acccttcgcc ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta atcaactccg
     2401 ccagcaactg ataaaccttc atcgtttctg ccggttcggc atcgccgcta tcgctgatat
     2461 acccttcatc acgcagcgta agcaccagag aactgaacac cgccttgtcg aagaactccg
     2521 gcgcgttgat gccgtgcagc acggagagac gttgcgcgac ggtgcggctc tctttctcca
     2581 gcgtaccgcg gttgatcgac gggttggcgc tcaacagcca gaaggtgatg gcataacgtt
     2641 gcagcgtttc gcgcgcgcct gcggcgagca gctgtagcgt gcgagaatgc accgggttga
     2701 tatgcaactc atcatcttgc agggtaatca gcccctgacg ttgcatctca tttgccagcg
     2761 catcaataac gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc gctttcagca
     2821 ttgggtaaag cacattgacg tgctccatca atacgtcgcg ggagatgtgg cgatgctggg
     2881 tgacaattgc cgccatcagc gaaggtagca ccaacatatg cgcaatgttg ttgcgatagt
     2941 aggtcatcag caccgcttgc tcgcgcggca gaatgatgat gtcgccgata gtgtctttct
     3001 caacttcaaa cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag
     3061 gaacggtaga gtccgtggag taaggcacgt tgcgcatcag atccaggtag cagttgagtt
     3121 gctcggttaa ctgctcgcgg gtgagtgagc gctgacgtga tgccagtagc gcagtacagc
     3181 acaggttcat ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa
     3241 tattattgac cgtcggcgtt aaccatgccg gacgcactgc ttcgatggga tcgatagatt
     3301 cacgccagtc tggtacgtgc tggttaaggt aggtcatcaa cggcattggt tcaccgaaat
     3361 tgacgtaacc ctggccgaga ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc
     3421 tctctttctc tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact tccatgacgt
     3481 gctcataacc gatatagatc ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg
     3541 cctgaatggt catcgacagc gtaccggttt tcggatccag caaacgcccc gtacgggaac
     3601 gaccgccttc tacgaagtac tcgacggaat aaccacggct gaacagttcg ccgagatact
     3661 cacggaaaac ggtggaatag agcttattgc ctttaaaagt acggcgaata aagaacgcac
     3721 ccagacggcg gaaaatcggc ccggcaggcc agaaattcag gttgatcccg gcggcgatat
     3781 gcggcggcac tagcccctgg tggtacagca cgtaagaaag cagcaggtag tccatgtgac
     3841 tgcggtggca aggcacatat accagctcat gaccgtcgtg ggccagctgg cgaacgcgct
     3901 cggcgttatg gacgttgatg ccctggtaaa ggcggttcca ggtaaagccc agaatacggt
     3961 cagtcaggcg gatcatctcg taagagaaat tcgccgcaat ctcttccatc agcgcaatcg
     4021 cgttctgctg cgctttttca tgggagattt ttttgctgcg cgcttcatct tctaccgctt
     4081 tggcaatggc gcgggaggcg agcagcttat taaacagatc ctgacgagca ggcagacgtg
     4141 gtcctacggc agccagacgt tgacgggcaa agtgcatacg cgccacgcgc gccagtttct
     4201 gcgcgatagt tttatccgtg ccgtgttcat ccgccatacg gcgcagcgaa actgacggcg
     4261 agaaacgcac aaaactgtcg cgaccgagcc acagtacagc gaaaaatttc tgtacgccgt
     4321 taagcatacg cagcggcggg ttcacttcgc ctttttcacg ccccggcgcg cgaccaaaca
     4381 tcaccgacac tggcaccatc tgcacatcca gatttgggtt gctacggtgc aaatcgagat
     4441 agtcgtggaa cagcttaata gactcttctt tcggcgtgta ataggtgaat acacgcggcc
     4501 cgccgtgaat gaacacatag cgcggcagta gcgtgccgtc gatttccagc ggctctaacg
     4561 ggtcaggcaa gtcatgtgcc agacactggg cgcgcaacgt cagcaaatct gctttcgagt
     4621 tgtacggtaa aacgtacata attggacgag aggtatccag ccccagttcc ggggcaggat
     4681 ctgccggaat agacttgctt tttaccagga tgcttaatgg taaattcagt aatttgtagt
     4741 aaattcgtgg ccagccggac ataaacgatg taaagcctct ggttaataat gcaaatgcgc
     4801 ggcaaggata gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg
     4861 ttacgcaaac ccgaatcatc ggatttaacg gtacactgat attgacgctc ataatgtaaa
     4921 aaggttcttt caatggccaa taataccact ggattcaccc gaataatcaa agctgctggc
     4981 tattcctgga aaggtttacg cgctgcatgg atcaacgaag cggcattccg tcaggaaggc
     5041 gtagcggtat tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc gattacccgc
     5101 gtgctgctta tcagctccgt gatgctggtg atgattgtgg aaatcctcaa tagcgccatc
     5161 gaagcagtgg ttgaccgaat tggctctgaa taccatgagc tttccggacg cgctaaagat
     5221 atgggatccg ccgcggtgct gattgcgatt atcgtcgccg tgattacctg gtgcatcctg
     5281 ttatggtcgc attttggata acccttccag aattcgataa atctctggtt tattgtgcag
     5341 tttatggttc caaaatcgcc ttttgctgta tatactcaca gcataactgt atatacaccc
     5401 agggggcgga atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga
     5461 tcacatcagc cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt
     5521 ccgttcccca aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgtaattga
     5581 aattgtttcc ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc
     5641 actggtaggt cgtgtggctg ccggtgaacc gcttctggcg caacagcata ttgaaggtca
     5701 ttatcaggtc gatccttcct tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg
     5761 gatgtcgatg aaagatatcg gcattatgga tggcgacttg ctggcagtgc ataaaactca
     5821 ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg
     5881 cctgaaaaaa cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat
     5941 tgtcgttgac cttcgtcagc agagcttcac cattgaaggg ctggcggttg gcgttattcg
     6001 caacggcgac tggctgtaac atatctctga gaccgcgatg ccgcttggcg tcgcggtttg
     6061 tttttcatct ctcttcatca ggcttgtctg catggcattc ctcacttcat ctgataaagc
     6121 actttggcat ctcgccttac ccatgatttt ctccaatatc actgttccgt tgctgggact
     6181 ggtcgatacg gcggttattg gtcatcttga tagtccggtt tatttgggcg gcgtggcggt
     6241 tggtgcaacg gcgaccagct ttctctttat gctgttgctg tttttacgca tgagcaccac
     6301 cgggctgact gcgcaggctt atggtgccaa aaatcctcag gcattagccc gtgcgctggt
     6361 gcaacctttg ctgttggcgt tgggggctgg ggcgttaatt gcgctgctgc gtacgccgat
     6421 tatcgatctg gcgctgcata ttgttggcgg tagtgaagca gtcctggaac aggcgcggcg
     6481 ctttcttgaa atccgctggt taagcgcacc ggcgtcgctg gcgaatctgg tattacttgg
     6541 ttggttactc ggcgtgcaat atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat
     6601 cctcaacatt gtgctggatg tctggctggt gatgggactg catatgaacg tgcggggcgc
     6661 ggcgctggcg acggttattg cggaatatgc aacattgctg attggtctgc taatggtgcg
     6721 taaaatcctc aaactacgcg gaatctccgg cgaaatgctg aaaacttcct ggcgaggaaa
     6781 cttccgtcgc ttgctggcac ttaaccgcga tatcatgctg cgctcgctgt tgttgcaact
     6841 ctgtttcggc gcgatcaccg tacttggcgc gcgactgggg agtgacatta tcgctgttaa
     6901 cgcggttctg atgacgctac tcacctttac cgcctatgcg ctggacggtt ttgcctacgc
     6961 ggttgaagca cattccggtc aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt
     7021 ctggcgggca gcatgccgcc agtcggggat cgtagcgtta ctgttttcgg tggtttattt
     7081 gctggcaggg gaacacatca ttgcgttact ggcgtcgtta acccagattc agcagctggc
     7141 cgaccgctat cttatctggc aggtgatttt gccgttggtc ggcgtctggt gttatctgct
     7201 ggacggcatg tttataggcg caacgcgtgc tgctgaaatg cgtaacagta tggcggtggc
     7261 cgccgcaggt tttgcgctga cgctccttac gctgccgtgg ctgggtaatc atggtttgtg
     7321 gttggcgtta accgtctttc tggcgttgcg cgggctttct ctggcagcta tctggcggcg
     7381 tcactggcgc aacggtacct ggtttgccgc aacgtgacgg ttaaaaattc tgaataaata
     7441 atcctaagcc aaattgctga ctacacttaa tctcacgttc agaagaaaag tgaacgtact
     7501 ctcattcaca acctaacgat gaggtcttga ttatgaataa agatgaagcc ggcggtaact
     7561 ggaaacagtt taaaggtaaa gtgaaagagc aatggggcaa actgaccgat gatgatatga
     7621 cgatcattga aggtaaacgt gatcaactgg tcggtaaaat ccaggaacgt tatggttatc
     7681 agaaagatca ggcagaaaaa gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt
     7741 aattaatccc tcctgcccga cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt
     7801 tcacctgcac agagtgatca tgctggcact gttcaggatg acgacacgct tccacttcca
     7861 cgcatgccgc acagagccca tgtgcttcaa tcacattatg ccgcagggca aaccccattt
     7921 ttgccgccag cgtatgcatg atgtcttcca cgccttcagc acattcttct ttcaccgcgc
     7981 cgcagcgatc gcaaataaac atagctgacg tatgggtggg ctggtcgaac agatggcaga
     8041 gcacataact gttggtggat tccaccttat gcacaaaacc ttgctcaagc agaaaatcca
     8101 gcgcgcgata aaccgttggc ggcttggctt gcggttcagc ttcgcgcagc aaatcaagca
     8161 gatcataagc gctgatagcg ccatcctgca gactcatcag gcgcaacact tccaggcgct
     8221 gtggggtcag gcgcacatta cgctgcgcgc agattttgtc agcctgcgct aataactcct
     8281 gcgttgtggt cttttccatc gggcacctca aagtgggggt taagaatgtc cttactttac
     8341 catgttccag gaaaaacacc gagaaccctt attgttgccg taatgttgct tttctgtttt
     8401 gtaggtaaga tgttgtgttg cctggcagta ccattatcaa cacgataata attaataatt
//
LOCUS       DAZKIF010000165         8424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZKIF010000165 DAZKIF010000000
VERSION     DAZKIF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.166"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(496..1068)
                     /gene="hdeD"
                     /locus_tag="ACHOTW_004149"
     CDS             complement(496..1068)
                     /gene="hdeD"
                     /locus_tag="ACHOTW_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP9846155.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1323..1655
                     /gene="hdeA"
                     /locus_tag="ACHOTW_004150"
     CDS             1323..1655
                     /gene="hdeA"
                     /locus_tag="ACHOTW_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP9846156.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            1771..2097
                     /gene="hdeB"
                     /locus_tag="ACHOTW_004151"
     CDS             1771..2097
                     /gene="hdeB"
                     /locus_tag="ACHOTW_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP9846157.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2161..2808
                     /gene="yhiD"
                     /locus_tag="ACHOTW_004152"
     CDS             2161..2808
                     /gene="yhiD"
                     /locus_tag="ACHOTW_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9846158.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2860..3658)
                     /locus_tag="ACHOTW_004153"
                     /pseudo
     CDS             complement(2860..3658)
                     /locus_tag="ACHOTW_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
     gene            complement(3655..4647)
                     /gene="shuU"
                     /locus_tag="ACHOTW_004154"
                     /gene_synonym="chuU"
     CDS             complement(3655..4647)
                     /gene="shuU"
                     /locus_tag="ACHOTW_004154"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFP9846159.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(4696..5319)
                     /gene="chuY"
                     /locus_tag="ACHOTW_004155"
     CDS             complement(4696..5319)
                     /gene="chuY"
                     /locus_tag="ACHOTW_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFP9846160.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(5319..5813)
                     /gene="hutX"
                     /locus_tag="ACHOTW_004156"
     CDS             complement(5319..5813)
                     /gene="hutX"
                     /locus_tag="ACHOTW_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFP9846161.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(5826..7163)
                     /gene="hutW"
                     /locus_tag="ACHOTW_004157"
     CDS             complement(5826..7163)
                     /gene="hutW"
                     /locus_tag="ACHOTW_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFP9846162.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            complement(7183..8097)
                     /gene="shuT"
                     /locus_tag="ACHOTW_004158"
     CDS             complement(7183..8097)
                     /gene="shuT"
                     /locus_tag="ACHOTW_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFP9846163.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
BASE COUNT         2263 a         1995 c         2039 g         2127 t
ORIGIN      
        1 gttaaaaagt aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg
       61 gattgccagc tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt
      121 caggttttta tatagcgtct ggagacatgg atatttatgc aatgtaatca acgccagttg
      181 caaaaaaatg aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc
      241 tttgttcact ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc
      301 atgataatgt ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt
      361 aggttgaaat aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac
      421 actgcgtagc ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg
      481 tgacaaccgg gataattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc
      541 ggcgctaaat atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg
      601 cgttgcacca aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag
      661 ccagctgcct ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac
      721 acagaacaaa ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcgcggat
      781 gaagaaatag ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca
      841 gaaattatga ctgcggttgc tgaataatcc aacaataagc gcaatgcccg aacagattaa
      901 taatgcacct actactgtgc ttaaaatatc gccagaaacg aacgggaaac tgatacacag
      961 caacccgacg ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag
     1021 catctccaga tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta
     1081 taaaattaag aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca
     1141 gtcgatttaa taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg
     1201 aaataaaaat atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt
     1261 ataacctcag tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta
     1321 cgatgaaaaa agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca
     1381 atgcagcgga tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactcc tggacctgtg
     1441 aagatttcct ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc
     1501 tgaacaacaa agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa
     1561 ccccagctat cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta
     1621 aaggcgaatg ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact
     1681 tccttgcata ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat
     1741 atataatctc tatattgaat gggttacaaa ataaatattt catctctccg taaagcgttt
     1801 atttttatgg gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gctggcagcc
     1861 aatgaatccg ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg
     1921 accccggttg catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgatacagtt
     1981 actttaaatg aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac
     2041 ccgcagaaaa atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga
     2101 ggtacaagta aaaaggagtg gcaagttgag ccatcttgct gctccttttt gcatttttat
     2161 atgacagcag aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc
     2221 atggaaaggc aaatgcgcgg caaaggagca gggttacgca ctcatgtatt aattggcatg
     2281 ggaagcgcac tgtttatgat tgtttcgaaa tatggttttg ctgacgtgct gtctttagat
     2341 cacgtcggac tggaccccag ccgtattgct gctcaggtgg tgacgggcgt cgggtttatc
     2401 ggtgcaggta acattctcgt tcgtaaccaa aatattgtag gtttgacgac ggcagcggat
     2461 atctgggtga ccgccgccat aggtatggtt attggcagtg gtatgtacga actgggtatt
     2521 tatggttcag tgatgacctt gctggtgctg gaagtcttcc atcaattaac cttccgcctg
     2581 atgaacaaaa attaccattt acagctgaca ttagtgaacg gtaatacggt atcaatgctc
     2641 gactggttta agcagcaaaa aataaaaact gatttagttt cgttgcagga aaatgaagat
     2701 catgaagtgg tcgccattga tatccagctt catgccacaa cgtcgataga agatctgttg
     2761 cgattattaa aaggtatggc aggggtgaaa ggcgtttcaa ttagctaatc tgtatagtta
     2821 tttattcgca ggagtaccag tgcggtactc ctgttgcttt taatgatcga ggacgatcag
     2881 tggtgaatgg ttggcaggat ctttaagtac ggtaatatcc gcgccgtaga gcattgtcag
     2941 cgcctgctgt gtcaatacgt cctgtggttt accatttgcg ataacctttc ctttttgcat
     3001 taatacaaca cggtctgcat aatgcgcggc aagatttaga tcgtgcataa tgcgcggcaa
     3061 gatttagatc gtgcaatatg cagcagacat tgaactgccg ctcgtgaact aactggcgta
     3121 ataaacgaaa caagtgctgc tgatggtgaa tatccagcgc cgaagttggt tcatcaagaa
     3181 ataaccattt cggtgaggga gttggttccc agagttgcac cagcaggcgc gctaattgca
     3241 cgcgctgttg ttcaccaccg gataattgcc gatagtcgcg gtttgctaac gcctgacagt
     3301 cacataacgc cattatttgt gctgtttcat tatcctgatt accagtacga tgcggatgcc
     3361 gacccatctg gataacttct tgtacgctga atggaaacgc catgtggcta ttttgacgca
     3421 tcacggcccg gtgtttcgcc agttcagtaa tcgaccattc atttaatggc ttattaaaca
     3481 accgacattc gccgctatca ggttgaagat agccggttaa ctggcgaagc agcgtagatt
     3541 ttcccgcacc attagggccg agaatggcaa caatttcccc accgggaaaa gtcagcgaga
     3601 cattatcagt cagacgccgc ccctgaaggc tataaaccag attctgggcg ctgatcatgt
     3661 gatcctccga tttcgcagaa tcaaccacat aaaataaggg ccaccaagca ggctggttaa
     3721 taatcccacg ggcatttctg ctggctgtac cagcgtgcgg gcaagcgtgt ctgccatcag
     3781 caataaacag gctccggcga gggcggaaca agggatgagc cagcggtgat ttgctccggt
     3841 agtcatgcga atcagatgcg ggatcaccag gccaataaag ccgataatgc cgcttaccga
     3901 tacggcagca ccaacgagca gtgagctcac taatagtaat tgctggcgtt tacgcttaac
     3961 gttcacgccg aggtaatggg cttcttcatc accgagctgc agtaaattca gcgttccggc
     4021 gagacaagtt gttgcgataa tggccggaag gatgaatgaa ctggcaacca ataacgtcgg
     4081 ccattgcgcc tgtccaagat tgcccatcat ccacaatgtg agttgtcgta gctgctgctc
     4141 atcgccaata tagctcagga tgccgatcgc tgcgccgcac agggcattga tggcgatacc
     4201 ggcgagtaat aattgcatca tgctgccatc gcgatggcgc tgcgtgatga gaaaaatgat
     4261 ggcgcagact actaaacttc cggcaatggc aaacaccatt tgctcataga gcaccagcac
     4321 cactgggaag gagaagggca gcacaattgc aacgccaacc attaatgcag aaccgctact
     4381 gacgccgagt aatcctgggt cagccattgg gttgcgaaaa agcccctgca taattacccc
     4441 tgcggttgcc aacgctgcac ctaccagcac cgccagcaaa acccgtggta gacgaatatt
     4501 gagccagata tcgcggtatt cctcatcaaa aagggcatca aaactgatct ttaacgctcc
     4561 ctgactagca ccgaacagca ccagggcaag caataatagt gatagtccca taaagaccct
     4621 ggcggatgaa aatgaatcct tgagcacgca aaactccgtt acgacattac ggcgcggaca
     4681 tgccgcgccg gggtatcacg ccggtttcag gtccggttca atcagactgt agacctgctg
     4741 gttaagtgct ggcgcatttg ccagttcatg gatatgtgcg gcgacatcgg cacggcggac
     4801 aaaaccatga cactcctgat tttgtatccg ttgtgctttg cctgttgctg cgccgtcaag
     4861 cagaccacca gggcggagaa tggcgtagtc tagctggctg gtttgcagcc agctttccgc
     4921 cagcgttttt tcccgtaccg cctgaccaaa cgcagccttt gcccgctccg ataaaaacgg
     4981 ccagctatcg ccgcatccca aagaggtcac cagaatcatg cgactgatgc cggccttttc
     5041 ggcctcatca atcactgttc tgtgcgccag ataatcctga gctcctccca ttgttgagat
     5101 gataagcgca tccgggccag ctgcgcggca agcagccgcg actacgctgg catcacaggc
     5161 gtcgcctgta aaaacctgta cgccttgttg cgccagtttc gtggcggcat cggcatgacg
     5221 aatgacagca acgaccggac gttgttccgc cagcgccagt tcaagcgttc tggcacccac
     5281 accttttccg cctgcgccaa agagtagcca cggcgtcatc aggcgtgttc ctttaacgat
     5341 gccgccagag tatggaaggc gctgacttgt tcgctcagga gctgacgatg gtcatccctg
     5401 ccgaggaaga ttttcagcat ggcgctgcct tctttgttga aaaagagaat ggacgcggtg
     5461 tccatgccca taaatttacg ttcgattaag gcaatatgcg tgcagttttc tgctttgata
     5521 tgcccggaca tgccgtgctt tccgcgtagg ttgaaatagc cgtggcgatg gaaacccgaa
     5581 ggcagttcgc cgctaaattc gaggattaca tcggcagtat gtactaacgt ggtgacgttg
     5641 ccccattcgc agacggtatc ccagacagta tcgaatttat cgcctggcac tacggtagag
     5701 gagggtagat ttctcactac ctccagcagt gtggtgttgt attgttcggc taccacctcc
     5761 agcgtaccgt ccggctccgt tttcaaaaat tcttgtaacg agacatggct cataaatcac
     5821 tcctgttatg gtttttcacg cataatcgct ggcgcattaa gtacctgaat tagttcatta
     5881 agagactgca aaatattgct tgcccagaaa cgaccttcat tagtcagccg caggcaagtt
     5941 gaagcatcgc gacttaagcc tttttggtgc cattgagcca gtaacggcgc gagtttttcg
     6001 gcatgtggcg taagttcatc cagcggtacg cgcgctgttt caacacctga ctgcaaggta
     6061 tgacgccatt gcgcattacg ttcggcgtta cgcatgatca acatcagcgg ttttttgcct
     6121 gccgcgacgg attcatgcca ggtctgaaga ttgcgttcgt tcatccagga gtaaccatta
     6181 atcgaaccgc cggctccgga accaaaggcc agacaatcgg caccttgttt aatcagcagg
     6241 ttatagagat tgcgttcgcg tgtggtacgg ccccagtggc tgttactgat gcagcgccaa
     6301 ccggcatcgt ccataaaatc acatccttgc agataaagat cgcgacgttc tgccggtgag
     6361 ggtacggtag tacgcccatt ttccacggcc ttgcccagcg gtgtattgga caggacattg
     6421 agcgcataga gatcgacgcc gtcgagaccg atatcgcggg caatagccag atcttctccc
     6481 caggtttgcg catcctgacc cggcaggcca aacagcaggt cacagaccac cgcagcacgg
     6541 tcacgtttga ccaggctttc cataaacgca atagcggttg ggccatccga ggtgcgggcc
     6601 attttcttgc ggattttgct gttaaacgac tggataccaa ttgagaagcg gtttgctcct
     6661 gcatcaaggc aagcatcgat tcgctcagcg tcaaaattca atacccgtcc ttcgatagtg
     6721 atttcacaat caggggccag tggcagcttt tctcgtaatg tggtgatgat tctggccaaa
     6781 tcgtgtgccg aaagtgcgga aggcgtaccg ccaccgaaat agaccgcgtg gatgggggca
     6841 gactgatgca atacgctgtc cgcttccatt tctatttcac gaatcagggc gtcggtataa
     6901 tgagcacacg catcttcatt aaaacggttc tgataaaaac cacaaaacgt gcagtgtgtc
     6961 gcacaaaaag gaatgtgtag gtaaaccagt cgcttacgag gtgatgccgt ttgattgatc
     7021 atctcttgcc aggtctgtgc cagttgctct ttggcaaccg gaatggctcc acgaaacggc
     7081 atcatggcgc gcctgtcttt aaatggctga tcaccgtcta aagcaaaatg tggcgtgaga
     7141 tccagggtgt tatttgcgtt cattcgtctg atttaaatcc atttattgcg gccaaagctg
     7201 ctggtgtaaa gactcaacga catcagcaat acgcggaccc attcccagaa tcaggttttg
     7261 atcaacggtg ataatgcgct ggtttttcca tgcggcagtg tgggtaattc cagcaataga
     7321 acgtagccga ttaatatcgc catcgaccat ttgtgaggtt acgacaatca cttctggatt
     7381 agccgcaatc agtgattctg cactgtagct tttgtactgc tggtgggtag cgacgttttc
     7441 tgctccggca agcgaaagaa tggcgtccgc gacgctgcct ttaccggcaa cttgtggcgc
     7501 acttccgcct gcggagagaa taaacatggc tttaaccggg gcttttttgg tcgccacgtt
     7561 ttgctgtact cgctccaggc gttggctgat ttgtgtcacc agcgcctcgc cttgttcagg
     7621 aacctgtagc gtttttgcca gttggcgaat gttggcgtac atctgctgaa gggtggcagg
     7681 cacacgcggc agggtgacga cgttgacttt ctgcgctcgt agttggtcga gcacaatttg
     7741 tggcccggca tcctgccagg taattacgct atccgggcga agtgacaaaa tgccttcgct
     7801 gctgagttgt ttccagtagc caatatgcgg cagtttggcg gtttctggtg gataagatgt
     7861 cgtttcatcg acaccaacca cgcgttcgcc agcgcccatc gcgtagatta gctccgtcag
     7921 cgatcctcct gcgaccacga tacgttcggc agccgttacg ctaaaagccc agctaagagc
     7981 cagtactgcc gtaaacaatt ttttacgttt tactgcggtc ttcgcggcag aggccacggc
     8041 tgaaatacat aacgtcagtg gggaaaaaag aaacggcctg gtgatgatcc ttggcataat
     8101 atgagaactc ttatcaaatt gagaatgatc atgattatca tttgagttat atatgaaata
     8161 caaccgtcga ttcaatacgc aaggcgttac aagcgtattt atttttgatt tttatcaaga
     8221 tgcttcgtca gctaacgttc ttgtttccga ctaaatttca tcgcgggaaa aagctgagaa
     8281 cccagagata tcgaggcttg cagagcaaag agaagaaaaa attatcactg ttaacgacaa
     8341 aatttagtga tgaggcatgg ggggataacc ataaaaacaa ggtggtttac agggcgcagg
     8401 ataggtttca taaccccctg ttta
//
LOCUS       DAZKIF010000166         8354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZKIF010000166 DAZKIF010000000
VERSION     DAZKIF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.167"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..207)
                     /locus_tag="ACHOTW_004159"
     CDS             complement(1..207)
                     /locus_tag="ACHOTW_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9846164.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENSRKHVA"
     gene            complement(307..1158)
                     /locus_tag="ACHOTW_004160"
     CDS             complement(307..1158)
                     /locus_tag="ACHOTW_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFP9846165.1"
                     /translation="MKNWSVEHTREVKKWLDIDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1585..6171)
                     /gene="ypjA"
                     /locus_tag="ACHOTW_004161"
     CDS             complement(1585..6171)
                     /gene="ypjA"
                     /locus_tag="ACHOTW_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFP9846166.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVYGGVSNGSLIESGGYQDVGRHNNYVGQSNNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTIKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIIDNTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNQGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPEPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSPNDIPEGIATLGAFMGYSHSH
                     IGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYH
                     SNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELG
                     ATLSYNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKA
                     SFSSTLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(6236..6355)
                     /locus_tag="ACHOTW_004162"
     CDS             complement(6236..6355)
                     /locus_tag="ACHOTW_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9846167.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(6579..6737)
                     /locus_tag="ACHOTW_004163"
                     /pseudo
     CDS             complement(6579..6737)
                     /locus_tag="ACHOTW_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(7107..>8354)
                     /locus_tag="ACHOTW_004164"
     CDS             complement(7107..>8354)
                     /locus_tag="ACHOTW_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFP9846168.1"
                     /translation="SIVKLICKEAASETYRGALEILQKIMSECIYQEGNAFVIMGAGE
                     QLKRIKYDVDENNLKVFNVHFDNNEVLVTDGEPDVVCLSKQVWENLLIKLKPEIKENV
                     ASEVHKSANKGEIEQLVEWSKRNEQTLFDNIIKSDFHVGSLKPGSMNGVILEMPPNVC
                     MEPRNSYENKIDEVSSLSESEEHPIDIQKITDAFVKEFKGILFDKNGRSSELLFNFYE
                     CCYTFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNVGFDFKLFPEVKLSGEHLETV
                     FKYKNGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGMSL
                     FDVDTPCIFNTPANHESYEKSLKPVSENGLNGVLSDRNKKIKMITGVAPFDDILFMDD
                     DFDDNSPEDAPIENSPVVNSPLV"
BASE COUNT         2124 a         2043 c         1724 g         2463 t
ORIGIN      
        1 tcaggcaaca tgctttcttg aattttcgat agttaattga atccattcgt ctacttcact
       61 ttcaatgaaa gcaacagagc gcgcaccgat tttaacgggg gaaggaaaac gattttggtt
      121 gatgaggtga tagatccaag ctttaccgta tccagttcga tgcagaacct ctggtaaacg
      181 aataaggcgg gtgttgtgct gattcatagt cgtttcctct gtgccgtttt gatggaaatg
      241 actatacaaa tgaatcagca ggaaaaagac cgaagctggg aacgccagag ctaatggagg
      301 ttggtcttag tcagaatcag cgattttcat cacgtctgaa actctgatct ttttcaaatg
      361 gctgttcttg ttgatgaaca gattaaactg gcgaataaaa ggtatactcg ccgccttcaa
      421 agcaagaggt ttgtctgtat ccctgatttg gtggttcagg cgagtgttga tctcatcatc
      481 gtcatctgta tacaacaggc gagaaaggcg gtcttctgat atacgtactt tatataattt
      541 tgaccatacc agaatgtcga tcatgggaag gagtctgtaa ttaatgattt tcttaatcgt
      601 tccataacca aagcgaatag cttccgttac atctggttct atgccacgca ctttacgcca
      661 ttgaggaagt gcagccttta gggattcagc gatctcttca tctgtcccgt ttgctaggtc
      721 gatttcgacc atgacagtat ccctgaacaa ttttggcaaa acttccgata cagggctatc
      781 gaggaattct ctgttaacag aatactcatc gcttccatcc caaaaaaaca gtgagttccg
      841 taatccaact atgctgagtt gagccagtct ttcggttgtc gtcaaaaaga agtgcggggg
      901 ctggtaaaga gtatcctctc ttgtgagata gccgagatgc ttttctgtga tgagaaaggg
      961 ctttccagtg aagatcctgt cgatgtagag ccttacggct tctgcttcaa attcttcatg
     1021 atatggtttg aagaacaatg tccttgccag caattcatgg tagaggtgta taagcggaag
     1081 ctcttcgaat ccgcgatatg tatctatatc cagccacttc ttcacctctc tcgtatgttc
     1141 aactgaccag tttttcataa cgatctccaa caggcattag acttaatgta tgtagcaaac
     1201 gtaatcgaca tgttaaagga agaccaatga aatgagttca gatgccatca ggaagttgaa
     1261 ccaaaaggtt tgatattttt tgatacagtt cgctaacgga ctattttcag gggtttatgc
     1321 cgttacgatt tatggttcct ttatagaacc aaaggtaaag tcagaaacag cagttatcta
     1381 gaaaattcaa tcaaatagat atctagttac agggacgcca gccaatcatg attggacggt
     1441 gtaaggacaa caccaacaaa aacaggaagt tagaagtctc agcaggacac cgaccagacg
     1501 gtgaggagac ataaaaggat acgcaaagga gccgcggctc ctggtgacat gaaagcccac
     1561 agatgtgggc tttttcgttg atggtcagaa cgaccagttc acaccagcta ccgcgttcca
     1621 cggggattcc acaccggcac catggctata ccccacccca agatgcccgc ttaacgtact
     1681 gctgaatgag gctttaatac ctgcctggta tattccacgt ctgcccgaca aatcattgac
     1741 gaaattaccg tcattattca ccttcacccg gttatcatcg acaaattctt tgcgcacagc
     1801 cgccttcagc cacggctcaa tttccatacc gttccccaga cgcatgttgt aactcagcgt
     1861 tgcgcccagt tcacgatata tactgcgggt atcgactgat ttcgattcca tgccattgga
     1921 taaatgatat tcggggttat cagcggtgaa ccccgttaac gatgcatacg gcgtcaggtt
     1981 ccagttacca tcggtaaatc gcatcccggt ttcaatgtga ccgcccagcc cgttgctgtg
     2041 gtaactgcca ttggcggctc caccgctgct cattttaccg gctacgttac tttcaaaacg
     2101 gttcagcttc acgacaccgt ccagatagaa accactttca tgttcccaac tggcatagcc
     2161 gcccagagaa taactgccca cactgccatg tcctccgcga tcaaaaccga tatgtgaatg
     2221 ggaataaccc ataaaagcgc ccagcgtcgc aatcccctca ggaatatcat tagggctgtc
     2281 gatccccact gtcattccgg tcagcgtctg ctcaaacccg gcccccgcat cggtggtgac
     2341 attattacgg gtgttatacg tcgcccccca gacattattg ttgtgtggac tcgctttcat
     2401 tatgttcaac cgctcgcgaa tactgtttag ctcagcatca aataccaacg gtaatgttgc
     2461 tgccatattg agtacggctg cggtagaagg cgtgatgcgt ttctccggaa cgggtgtcgg
     2521 cgttggctcg ggagtcgggt ctggtttcgg atccggtttt gggtctggtt ttggatccgg
     2581 cttcggattt gggttgggat ttgggttggg atccgggttg ggtttgacat cattggtcag
     2641 gttccagttg ctgttgccat cgcttttcag gacatactca taggtcccaa gatcaacgaa
     2701 accgccagta ttgcccagcg aaaacgaagc atcccctccc cctgttttca ccagcgtcat
     2761 cgcgtcgtca gactgaggac tgacgccggt atcctgaaca aagattttaa aattaccagt
     2821 ggcgttgttg ttgacgacca gttgatcgcc ccgagagcct gcaacgttgg tatgcaggta
     2881 gaaattaccg ctaccggaaa gttcattggt tgtcagcgta ttgtatatac cgttagttgt
     2941 ggcaggcgct gctgtttgtg ctgataaata aacattaccg tcgttaagta gcaaactgtt
     3001 tactctatac tcgcagttgc tggtgcctgc acaggaatta ttgctgttaa gccagacact
     3061 gccccggctg gcagccgtca ggtcggcaag cgtcgtatca acgccattgc cgatagttaa
     3121 tgtcgcgcta tcggtaatcc ggatcgcccc ttcgagttta actggcgtaa cattatcccg
     3181 tggcgtcatt aacgacaggc ttcctgtcgc gccactgacc gatgcatccg ccagcgtacc
     3241 tgcgtagacg attgctgtcc cgccagcaac ctggagatct tctgcccggg ccagagcctg
     3301 aaactcatct tttgaccgcc caagggtata ttgcccacca gagttaacct ttgtggcgga
     3361 gtcctgaccc tggttttgca ttgccccccc cttgccaacc gtggagtcgc gagcttcggt
     3421 acctgctaat accaataaat taccgccatt ttccagcaac atattggtcg ctaaattgcc
     3481 ggaaatggaa aaagtgccgt actggtgagt accgctgatt tcaataccgt tagccgtgct
     3541 cgtctggaga gcggcaccgc tgttctggac gatatctgtc gctttgccat tatcgttgac
     3601 tgtcagcgta ccgccttcat tgatcgttgt ttttattgcc tctccgttag ctgaaactgt
     3661 ttgtattccg ccgtcgttaa ttgttgtctc attcgccaca ccctcgacaa tttgttcacc
     3721 gccggtgagc gtcgtgcctg tcgcagtggc ttttgttttg acgatctccc gtccgcccgt
     3781 attgacctgt gttttgtcag aagaggtgtc tgactccacg gttaacactc cgccatttgc
     3841 cagcaggata ttgttcgccg caccctgctc gatgctgaac gcgacgccat ccgcgcgtgt
     3901 tcctgtgacc cgcgtcgccc tggtggttgc aaccaacgcg ccctggctac tctgctgtat
     3961 ccccgttgcg ctgcctttct cccgcacatc gagtgtgccg ccatcattaa gcaccgagtt
     4021 ttcagccaga ccgccctcat taactatctg tgaaccgcca ttaataatgg aaccttccgc
     4081 tgtcccgttt gccataattt gttgtaggcc agagacgata tcggtattga tcgccttacc
     4141 ataattctga acggtttgcg tgccaccatt gatgtgtgtt ttctctgttg acccaccatc
     4201 aacaatttgt tcaccacttt cgatatttgc ttcagtggct aaaccatata ccgtttgctt
     4261 gccacctttg atatttgctt tatcagaagt ggcactggca tatattgttt gggtgccagc
     4321 actattgagt acagtgccaa catcttttcc ataaacatcc attttgccgt tggcattaat
     4381 aatcgtgtca actgcccggg aaccagtgac gactgttaat gagccagcgt tttccagcac
     4441 tacattttta gcttctgaat tcctgatgta gaaagcatca ccataactgt tggttccttc
     4501 gataagtgtt ccggaggtcg tggaagcaat taatgcgccg ccggattgtt gctcaacatg
     4561 cttagcctca ccaccgtcct gaacctccag aacgccgcca ttattaagtc tggtgctatc
     4621 tgttttagcc tccttctgga caatcagctt accgccagca tcaatggtag tatttttcgc
     4681 cgaggtttta gccactaccg tcagttcgcc ggtattttcc agcacaacat aattagcctc
     4741 ccctccggta atagtgaagt gagagagctt gttgtatcct tcgatatcag tccctgcacc
     4801 cgtgttggca actaaagcac tgcccgtctc ctggttaacc ccatgtgcaa taccgccggt
     4861 atagacaatc agcgagcctc cggcgctaat attgctgcca attgccgtac catctttctc
     4921 aacaacctgc cggctcccgg aggatataat tgttgtgtca gctttcccgc cgcttttgat
     4981 attttgcgtt ccgctgttga tattggtgcc tgtggctatg ccataattat taatattctg
     5041 tgtgccacca ttaattatgg tatttgtcgc gtttcctgca acatccataa ccccgccatt
     5101 atctattcgg gtcgcatcag ctttagcatt ggttaaaact gacattgttc ctttatcttt
     5161 aataatcgtc ttgtttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag
     5221 caacacattg tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct
     5281 caccgtcgta ccgttagtgt tagtttttaa aattgcacca tcgtgctggg taacatttgt
     5341 tgccgtacca ccactaacat caagcacgcc accggaataa acttcaataa catccgagga
     5401 gctggtgtta tcaataattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga
     5461 cttactattc agagactgag ttccgccttc aatcgtcgtg tccagcgcac ggctctcata
     5521 tactctttgc tcaccgccat ttttgatggt ggttgtttct actgtgctct gttcaacata
     5581 ctgtcgacca ccatttatgg ttgtgttcgt tgccagactt ccttgtacta cgtcctgaga
     5641 gccagactta tttatcgttg taccatcagc atgcccttga acttttacta tctggctacc
     5701 accatcaatg agtgttccat tcgcactccc tccctctacg cgtgaagcac cgcccttaat
     5761 tgtcgttcca ttgctgatac cccctttata aacgtcctga ttgccactct cgattatcgt
     5821 acctgtggaa atacccccgt catgaattga ctgtctgccc ccgttaatgg tggtattatt
     5881 agactgcccc acataattgt tatgccttcc tacatcttga tatccaccag attcaataag
     5941 acttccatta gataccccgc cataaacatt ctgctggcca tggttgataa tatgagtgtt
     6001 atttgttgta cctcgttcat ctactcgttg gctgccatct acagtctcat cgtttaccac
     6061 accaataaca tcaggagtga aggcagccat cccgggcggg gcatatatca aggcagatat
     6121 caataaggaa agtactgagc gacgacaata atagggactg gtcctgttca taaatttcat
     6181 cctctgaaaa gtgaatactg agtagcgttt aagcgacctt agctttgctg caacatcagc
     6241 ccacaggcac cagaccaggg gattcatcct gaagagacag cgcaagtgta ttgtgttcac
     6301 cgctcatcaa agacatcatg atgaaatgat gatattccgc ataagaatga ggcatttttt
     6361 aaacgcagtg cgctgaagtg tggttggata aaaaagtcaa tccattcagg aaatacgggc
     6421 gtattctttt ctttcgacaa tgaggccgtt ggcaaaataa aatgatttac ataatcgttt
     6481 ctgatgaata tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattgtt
     6541 ttacttttac agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga
     6601 aagttaatac cacgttcgca cagctcctcc aacagcacga caagatgcca catactgcgc
     6661 cccagtcggt tcagtttaca gactagcagt gctttccctg ccgatgatgt cctgagcagt
     6721 tttttcagtc cggacctcct gccacggttt tacgttgaag cggtaaccct gagcacgcag
     6781 ttcttcagtc aggcgtggtg caccgtaacg ctgttattgc tgggtgagat caaaaaactt
     6841 tcaggcagct aaggaaagtt gaaccagaca atagatgaaa tatttcaacc aattacagca
     6901 ccaattcagt cactgccagc ccaccaaata aatcaagggg ttacatgaaa acgtagcccc
     6961 tttttctttg gtagtgacac taaaatggat gtagtgtgaa gaataatccc gtttactcaa
     7021 tcaataatac atattgtttc aatctacgtt attatctctt tataaaaatt gccatttatt
     7081 tatcattgaa aactgctttt agaactttat acaagaggac tattcacaac aggactattc
     7141 tcaatgggag catcctcagg ggagttatca tcaaagtcat catccataaa taaaatatca
     7201 tcgaatggtg ccacacccgt gatcattttt atttttttat tacgatcaga taagactcca
     7261 tttaaaccgt tttcgcttac aggttttaat gatttttcat aactctcatg gttagcaggc
     7321 gtattaaaaa tacacggagt atcaacatca aacaatgaca ttccccagtt cacatattga
     7381 atatcatagt tactgaagtt ctgtccagaa aagaagcatc ccttaaaatc caatccacgt
     7441 agattatata agccaccctc ttctttttgg agagtaatgt taattttggc tatctcccgg
     7501 acatcatcgc catttttgta tttgaatacc gtttcaagat gttctccaga cagcttgact
     7561 tctggaaata atttgaaatc aaagcctaca ttattatgtg tcaacgtaga tgaacaaaag
     7621 atggaaaaag cttgcagtgc tgaattatag ctatcgattt tatcttgagg ctgcgctctt
     7681 ggtaaaaacg tatagcaaca ttcataaaaa ttaaacagaa gctctgaaga tcttccattt
     7741 ttatcaaata atatcccctt gaactccttc acaaacgcat ctgttatttt ttgaatatct
     7801 atggggtgtt cctctgactc tgacaaagat gaaacctcat ctattttgtt ttcatatgaa
     7861 ttacgtggtt ccatacagac atttggtggc atttctaaaa taacaccatt catactacct
     7921 ggcttaagtg aaccaacatg aaaatcactt tttattatat tatcgaaaag ggtttgttca
     7981 tttcttttag accattcaac taattgctca atctcacctt tattcgctga tttatgaact
     8041 tcagaagcca cattttcctt gatctccggt tttaatttaa tgagaagatt ttcccagacc
     8101 tgcttgctta aacatactac gtcaggctca ccatcagtaa ctaacacttc attattatca
     8161 aagtgtacgt tgaatacctt taagttattt tcatcaacat catatttaat acgttttaat
     8221 tgttccccag ctcccataat gacaaaggcg ttgccttctt gatatataca ttcagacatt
     8281 attttttgta aaatttcaag agcaccgcga tacgtctctg atgcagcttc cttacaaatt
     8341 aattttacaa tact
//
LOCUS       DAZKIF010000167         8139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZKIF010000167 DAZKIF010000000
VERSION     DAZKIF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.168"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1727
                     /gene="adeD"
                     /locus_tag="ACHOTW_004165"
     CDS             <1..1727
                     /gene="adeD"
                     /locus_tag="ACHOTW_004165"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9846169.1"
                     /translation="AEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRY
                     IAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVI
                     CDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAW
                     RDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAGIE
                     NCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWE
                     IAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSD
                     ARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQPVSASDFALQFTPGK
                     RYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRLAPACGLLGGFGLNE
                     GALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLM
                     STDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFA
                     FTTLEVTE"
     gene            complement(1773..3164)
                     /gene="uhpT"
                     /locus_tag="ACHOTW_004166"
     CDS             complement(1773..3164)
                     /gene="uhpT"
                     /locus_tag="ACHOTW_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP9846170.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3302..4621)
                     /gene="uhpC"
                     /locus_tag="ACHOTW_004167"
     CDS             complement(3302..4621)
                     /gene="uhpC"
                     /locus_tag="ACHOTW_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9846171.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4631..6133)
                     /gene="uhpB"
                     /locus_tag="ACHOTW_004168"
     CDS             complement(4631..6133)
                     /gene="uhpB"
                     /locus_tag="ACHOTW_004168"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP9846172.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6133..6723)
                     /gene="uhpA"
                     /locus_tag="ACHOTW_004169"
     CDS             complement(6133..6723)
                     /gene="uhpA"
                     /locus_tag="ACHOTW_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP9846173.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6885..7988)
                     /locus_tag="ACHOTW_004170"
     CDS             complement(6885..7988)
                     /locus_tag="ACHOTW_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846174.1"
                     /translation="MNSYIKIDQTGSNKFKITDLYREFNGETKCYDKKCRDMDFSGRN
                     VKEFIRLLSDHDQFEKDQISELTVAANALKLEVAKNNYNMKYSFDTQTERRMIELIRE
                     QKDLIPEKYLHQSGIKKLKLHEDEFSSLLVDAERQVLEGSSFVLCCGEKINSTISELL
                     SKKITDLTHPTESFTLSEYFSYDVYEEIFKKVVFTPGECDLVRDSLVQDGIKPEKIAK
                     IISCLSSRTGIRNLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSFVALNGRNY
                     HQHVDFSEYGFTQTLCVLGAVVNNANDTFDVANYIYTTKGENIIHNDAEDDSPYVHSD
                     ESYRHKDDYWARDNDIRNLRRNFSGNITIATYK"
BASE COUNT         2124 a         2221 c         2000 g         1794 t
ORIGIN      
        1 gggcggaata ccaggaattg ttagccgttt cccgtggcga cgctgttgcc gattatatta
       61 ttgataatgt ctctattctc gacctgatta atggcggaga aatttccggc ccaattgtga
      121 ttaaagggcg ttacattgcc ggtgttggcg cagaatacac tgatgctccg gctttgcagc
      181 ggattgatgc ccacggcgca acggcggtgc cagggtttat tgatgctcac ctgcatattg
      241 aatccagcat gatgacgccg gtcacctttg aaaccgccac cctgcctcgc ggcctgacaa
      301 ctgttatttg cgatcctcat gaaatcgtca acgtgatggg cgaagccggt ttcgcctggt
      361 ttgcccgctg tgccgaacag gcaaggcaaa accagtactt acaggtcagc tcttgcgtac
      421 ccgctctgga aggctgcgat gttaacggtg ccagttttac ccttgaacag atgctcgcct
      481 ggcgggacca tccgcaggtt accggccttg cagaaatgat ggactaccct ggcgtaatta
      541 gcgggcagaa tgcgctgctc gataaactgg atgcatttcg ccacctgacg ctggacggtc
      601 actgcccggg tttgggtggt aaagaactta acgcctatat tgctgcgggt attgaaaact
      661 gccacgaaag ttatcagctg gaagaaggac gccggaaatt acaactcggc atgtcgttga
      721 tgatccgcga agggtccgct gcccgcaatc tcaacgcact ggcaccgttg atcaacgaat
      781 ttaacagccc gcaatgcatg ctctgtactg atgaccgtaa cccgtgggag atcgcccatg
      841 aaggacacat cgatgcctta attcgccgcc tgatcgaaca acacaatgtg ccgctgcatg
      901 tggcatatcg cgtcgccagc tggtcgacgg cgcgccactt tggtctgaat cacctcggct
      961 tactggcacc tggtaagcag gctgatatcg tcctgttgag cgatgcgcgt aaggtcacgg
     1021 tgcagcaggt actggtgaaa ggcgaaccga ttgacgcgca aaccttacag gcggaagagt
     1081 cggcgaaact ggcacaatcc gctccgccat atggcaacac cattgcccgc cagccagttt
     1141 ccgccagcga ctttgccctg caatttacgc ccggaaaacg ctatcgggtc attgacgtca
     1201 tccataacga attgattacg cactcccact ccagcgtcta cagcgaaaat ggttttgagc
     1261 gcaatgatgt gtgctttatt gccgtacttg agcgttacgg gcaacgtctg gctccggctt
     1321 gtggtttgct cggcggcttt ggtctgaatg aaggcgcgct ggctgcgacg gtcagccatg
     1381 acagccataa tattgtggtg atcggtcgca gtgccgaaga gatggcgcta gcggtcaatc
     1441 aggtgattca ggatggcggc gggttgtgcg tggtacgtaa cggccaggtc caaagccatc
     1501 tgccgttacc cattgccggg ctgatgagca ccgacacggc gcagtcgctg gcggagcaaa
     1561 ttgacgcctt gaaagccgcc gcccgtgaat gcggtccgtt acccgatgag ccgtttattc
     1621 agatggcgtt tctttccctg ccagtgatcc ccgcgctgaa actaaccagc caggggctgt
     1681 ttgatggcga gaagtttgcc ttcaccacgc tggaagtcac tgaataataa aaaaagcccg
     1741 gcgtcatgcc gggcaaaagt caccagttac gtttatgcca ctgtcaactg ctgaattttt
     1801 ttctcgcggc ggattttgcg ttcttccatc accgccacta tcgccatcag gcagatacaa
     1861 ccaatcgcgg cgatatccag cgcagcgaag gtgcctgccc agccggtaag gccgaatact
     1921 ggcgtcccat cggcaatcat tcccagacct aacttggcaa agctgtcacc aatcaggtaa
     1981 gcgaaggtgc ctttaatacc atcggcagcg ccaatcgctt ttttaggtac aaagccaaca
     2041 gcagccacac caatcaacaa ttgcgggcca aagaccagga aacccaacgc aaagagagaa
     2101 gccagataga tatattcgtt gctggcatgt tggtacacac cgagcgtggc gataatcagc
     2161 gccagcgcga tgcaggccac caggccacgg cgaccgttcg ctaggtcaga gagccagccc
     2221 cacagcagcg taccgaccag cgcaccagct tcaaacagcg taaagccctg aatcgccacc
     2281 gctttagaga gtttcagttc ctggaacgcg tacacagttg accactggtc gataccaata
     2341 cgcaccacat agaggaaaat gttggcgaag cacagcagcc agatcacttt gtttttcagc
     2401 acatactcaa caaagatctg ccacttggtc atatcggtag attctgtctc tttgtcctct
     2461 tcgctgatct cctcgccgaa cagctcttca gctttgccga ggccataaga ttccggggag
     2521 tcgctgccgt aacgcaggcc gataaaaccg acaatcagcg cgataatcga cgggaagata
     2581 aacatgccga tgacatggcc atcgaacagg taatttgcgc cgaacagtgc cacaccagct
     2641 gcgcctgcac cgccaaggtt gtgagaaatg ttccagaaac cgaggaacgt cccgcgttta
     2701 cgacgcggcg tccatttggt gatggtggag taactgcacg aacccccggt actctggaaa
     2761 aagccactta aggcgtagaa ggcaatcatc aggaacaggc taaccgagcc gctgcccata
     2821 ctggcgctaa agcccagcat acaaatggca gacaggatca gcatgaacgg caggaattgt
     2881 ttggtgtttt tgccgtcggc gtagtaggaa accagcgttt tacccacgcc ataagtgatg
     2941 gagaaaccca ggccgatcat ccccagctgc gtcatgctca acccgtaggt cgaaatcata
     3001 tcgttctgcg cgatgttaaa gttcttgcga atcaggtaca tcgtcaggta gccgataaag
     3061 accaccaggt aggattgcat gaacggtttg aaccacattt tgcgccgcac ttcgagcgga
     3121 aggtccaggg tcggcttgcg gacctggttt aagaaagcca gcatgggtta ctcctgaaat
     3181 gaatacctgc gtgaggcatg cattgtaaaa atcagcagca aatcaggcct gagatagcgt
     3241 ccaggcaaaa cctggaaaat ttcttagttt tgccggatat gaagtgaaaa ggtgagatgc
     3301 atcacgcttc gcgcggtgtc tgggcgttca aaaagggcag taacagcagt gcggaaatcc
     3361 cggcggcaat ggcgataacc acaaaaaatc cgctccagtg ccaggtatcg agcactttcg
     3421 ccagcggcca accagcaagc gacgccccca gataagcaaa caagccgaca aaccccgtcg
     3481 ccgcccctgc cgcctctttg tgggaacatt ctgccgccgc catgccgatt aacatctgcg
     3541 ggccaaagac aaaaaaacca atggtgaaga agcaggttgc ctgcatcacg tagctggcaa
     3601 atggcatcag ccacagcgag ccgactgaaa gcaaaattcc ggcagcgaaa atcaaattca
     3661 tcggcccgcg gttgccgtta aacagtttgt ccgagcccca acccgctacc agcgcaccga
     3721 taaatccgcc cagttcaaac atcgtcactg ccgtattcgc cgtgaccaga tcgacgccca
     3781 gcgtctcgga catatacaaa ttgccccagt cgttgatcgc cgcccgaacc acatagacca
     3841 gcacatagca aaacgaaagc agccagatat acggattcaa caacacatac ttggtgagga
     3901 tctctttacg cgtcagccct gccccttctt gttgttgagc aatctccagc gcgtcgtgtc
     3961 gccattcacc gaccgctggt aaacctaacg cctgcgggcg atcgcgcagc cgccagcaga
     4021 gaaaaatccc cacgacgatc gccatacaac cagcaatcat catcccggca cgccagccgt
     4081 aatgcagcgc agctgctgcc atcacaatgg gaatgagcgc accgccaacg ttatgcgccg
     4141 tgttccataa tgcccaccaa ccaccgcgtt cggtacgaga ataccaggcc gttaacagac
     4201 gcgcacacac cggtgaaccc cagccctgga aaaaggcgtt cagcacccag agcacggcaa
     4261 acgcccatag cgacgtcgag aagccaaaca gaatgttgat gatgcccgtg gcgataagcc
     4321 ctatccccat aaaataacgg gcatttgagc ggtcgctgac aatgccggag acaaacttcg
     4381 acacgccgta ggtaatgtaa aacagggtcg ctaacaggcc gatatcgcta cggctgagca
     4441 cgccattagc aaggatttct ggtacggcgg cgttaaaact tttacgcgtg aagtaaaaca
     4501 gcgcgtaacc cagccagatg gtcagcagaa tatgccgacg ccagtagcga taacgggcat
     4561 caatttcatg tttatcagtc attaatggcg catcggcagg cgctttcaga aacggcaaca
     4621 tcatcaaacc ttagacatag cgttgaggta gagaaacgct gacacgcgtg ccgtgcagac
     4681 aggaaatggt caaggtgccg cccagcgccg ttacgcgctc gcgcattccg gtgaggccaa
     4741 aaccttgttg cccggaaccc ggcggtaaac cgctgccatc gtcttcaatc accagcatca
     4801 accgttcatc ctgcagccag ccttgcaggg tgaccgcgct ggcatcagca tgtttcacaa
     4861 tgttattcag cccttcctgg cagacacgaa acagcgtcac gcgctggttt tcgcttaacg
     4921 ctgactcttc gattcgccat tcgagatggc tgacaatgcc gcgcccttcc agctccattt
     4981 cccgcatcag tgagcggatg gcctgctcta gggtgagatc atccaactgg cgcggacgta
     5041 accgccctaa caaacggcgc accgcgtcgt acacgcccag cgacagttgt tcgatgagct
     5101 gcccgctctg cttcacactg gcgttatctg ccgccagtcg ctggacaatg cccgcttgag
     5161 tacgaatggc agtgatggtc tggccgatat catcatgcag ctcacgcgcc acatcacggc
     5221 gcacgctctc ttcagtttct aacaaacgtt cagcgagatg ctgattgcgc gccagttcct
     5281 tttgcagcga ctggttaagt tcacgcaacc gctggatgcc agcgcccagt aacaaccctg
     5341 tcagactttg caccagcagc gagagcaata aatccacagg atgatcgcgc caggtttgac
     5401 tggcgatcag cgcgatggcg ttcatcaatg tcgctatcag cgccccttgc cagccgtagt
     5461 gccaggccag cgcgataatc ggcagcgcca ggcagaatgg cgtaaagcgc gacagttcgt
     5521 ccggcaagcc caactggagc cagagactga taacaaacag cagcaagtac cagaccagat
     5581 gccgcccgcg ccagttgatc ggttgagaaa ctaacgacgg cccgagcggc agccaggtgt
     5641 tattggcgag ataatgccag aacaccagac atatcggggc cagcgtcagg ccgccagtta
     5701 aagttaacag cagcgcattc cacgactctt tgccgtgcca aagccagggc agcgactgca
     5761 acaacgccgc cgccgttaac gccgcaccct gtaacagcaa ggtgcgccag tcacgttgat
     5821 ggcgatagcg cgagatcagc gccacgggca gtaacgtcag taaactaccg atcatcaata
     5881 aggagaaatg ggttaaaccg accgcctgcg ttagccagta aatcagcagc cactccgctc
     5941 ccagcagcac cggccagtat ccgcgcgggc attgcagcat tagccctagc cgcagaccaa
     6001 acggaaataa cagcaccgcc atatcagggc gctcaaccag gtgcaggctg atactccaca
     6061 ggcaaaacca tgcggcagag aagataaaaa agcaggcaat aacggtaatt aagcgggaga
     6121 acaacgtctt catcaccagc catcaaacat acggcgcgcc agttcaacgt cgttactgac
     6181 gcccagtttt tccatcagat tggcgcgatg gacgtgtacc gttttcggtg acaagcccag
     6241 ttcggcggca atctctttca ccgccattcc ttgcgccagt ttttccgcca cctgccgttc
     6301 acgtttggtt agtgggtcct ggcgaccgga tgccagttta atggcaatat ccggcgtcag
     6361 ataacagccg cctgtggcaa ccgtatgcac cgcagcaatc agttcgtcag ggctacaacg
     6421 cttggagaga aagccacgcg cccccgcgtt aagcgcctgc tcaaccagcg ccggactgtc
     6481 atgaacggag agcataattg tcgccatacc tttcggcagc tggcttagca gctccagacc
     6541 ggagatatcg ggcatggaga tatcgcaaat acacacctgc acaccgcgcc ccggcagccc
     6601 cgccagcgcc tcgcgccccg aaccaaactc ggcaactact tgcaaatcag gttccagccc
     6661 cagcaactgc gcaaagccgg agcggacgat gaggtgatcg tctataaggg caacggtgat
     6721 catgattggg tcctggcgga taaaaaacgc gcatacctta acgataagcg cgatgttgtt
     6781 caagttggat atggggtatg tattggctta atgatgttga gcttatcgtc aaaacgctta
     6841 cctgagaggt aagcgtttgg tgcgtgttat tgagaaaaag cgaattattt gtaggttgca
     6901 atcgtaatat tgcctgagaa atttctcctt agattgcgga tatcattatc ccttgcccaa
     6961 taatcatctt tgtgtctata actctcatcg gaatgtacat agggagaatc atcttcagcg
     7021 tcgttatgga taatattttc gcctttagtg gtatagatat aattagcaac atcaaaggtg
     7081 tcattcgcgt tatttacgac tgcgcctaag acgcaaaggg tttgagtgaa tccgtattct
     7141 gagaaatcga catgctgatg gtaatttcta ccattcaaag caacaaaact cggacaaaca
     7201 tctattgtat cgccaacaga cgtagcggcg cgacgcacat caagtgcagc gttatcgtta
     7261 gcaacatgaa atgcttgtga acaggcataa aacaaatttc taatcccggt acgactggat
     7321 aaacaagaaa taatttttgc tattttttct ggctttatac catcctgtac taaagaatct
     7381 ctcaccagat cacattcacc aggtgtgaaa acaactttct tgaatatttc ctcataaaca
     7441 tcataagaaa aatattcaga gagagtgaaa gattcggtgg gatgtgttaa atctgtaatt
     7501 tttttgctaa gtaattcact tatagtgctg tttatttttt caccgcaaca taaaacaaat
     7561 gaacttccct ccagaacttg cctttctgca tctacaagga gtgagctaaa ttcatcctca
     7621 tgaagcttaa gtttttttat acctgattga tgtaaatatt tttctggaat taaatctttt
     7681 tgttccctta taagctctat cattcggcgt tcggtttgcg tatcaaaaga gtatttcatg
     7741 ttatagttat ttttagcaac ttccagctta agagcatttg cagcaactgt taattctgag
     7801 atttgatctt tttcaaattg atcgtgatct gataataacc ttataaactc ttttacattt
     7861 cgaccactaa aatccatatc cctacacttt ttatcataac actttgtttc gccattgaac
     7921 tcacgataca gatcagtgat tttaaattta ttacttccgg tctgatcaat ttttatatag
     7981 ctattcatgc ctgtaacggc aatctatgtc ttataattcg cattctccga ttcaggcgta
     8041 acatgaaaat aaggaaccaa tttatcacta atatcatgcc gatagatatg aaatgaatct
     8101 aaaagatata ataaaaaact tcgaaaaatg tcacgaaaa
//
LOCUS       DAZKIF010000168         8061 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZKIF010000168 DAZKIF010000000
VERSION     DAZKIF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.169"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(32..223)
                     /locus_tag="ACHOTW_004171"
     CDS             complement(32..223)
                     /locus_tag="ACHOTW_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9846175.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(220..408)
                     /gene="dicB"
                     /locus_tag="ACHOTW_004172"
     CDS             complement(220..408)
                     /gene="dicB"
                     /locus_tag="ACHOTW_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9846176.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            982..1167
                     /locus_tag="ACHOTW_004173"
     CDS             982..1167
                     /locus_tag="ACHOTW_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846177.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(1354..1743)
                     /locus_tag="ACHOTW_004174"
     CDS             complement(1354..1743)
                     /locus_tag="ACHOTW_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846178.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(1755..1883)
                     /gene="ydfB"
                     /locus_tag="ACHOTW_004175"
     CDS             complement(1755..1883)
                     /gene="ydfB"
                     /locus_tag="ACHOTW_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFP9846179.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(1885..2040)
                     /locus_tag="ACHOTW_004176"
     CDS             complement(1885..2040)
                     /locus_tag="ACHOTW_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9846180.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2318..2605)
                     /locus_tag="ACHOTW_004177"
     CDS             complement(2318..2605)
                     /locus_tag="ACHOTW_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9846181.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(2605..2796)
                     /locus_tag="ACHOTW_004178"
     CDS             complement(2605..2796)
                     /locus_tag="ACHOTW_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9846182.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(2824..3225)
                     /locus_tag="ACHOTW_004179"
     CDS             complement(2824..3225)
                     /locus_tag="ACHOTW_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9846183.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            3334..3606
                     /locus_tag="ACHOTW_004180"
     CDS             3334..3606
                     /locus_tag="ACHOTW_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9846184.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            3590..4015
                     /locus_tag="ACHOTW_004181"
     CDS             3590..4015
                     /locus_tag="ACHOTW_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9846185.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4222..4677)
                     /locus_tag="ACHOTW_004182"
     CDS             complement(4222..4677)
                     /locus_tag="ACHOTW_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846186.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            4756..5847
                     /locus_tag="ACHOTW_004183"
     CDS             4756..5847
                     /locus_tag="ACHOTW_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9846187.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMTAG
                     SEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANPKK
                     SAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPW
                     LLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            5854..6600
                     /locus_tag="ACHOTW_004184"
     CDS             5854..6600
                     /locus_tag="ACHOTW_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9846188.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            6622..7392
                     /locus_tag="ACHOTW_004185"
     CDS             6622..7392
                     /locus_tag="ACHOTW_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9846189.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            7408..7821
                     /locus_tag="ACHOTW_004186"
     CDS             7408..7821
                     /locus_tag="ACHOTW_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9846190.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT         2156 a         1720 c         2099 g         2086 t
ORIGIN      
        1 aatgaaaccg gatattaatt acggtgctgt tttaaagtcc tgccggtatt tcgttattat
       61 tagtgtgagt agttttatct accggataac agttaccggg aattttttgt tctgctgctg
      121 cagccatgca ttctttcatt gaaccgtata agccagtcac cagctcaaga gattcgccgg
      181 aaacaagata aactgtcaga acgagtgcaa atgttgtatt cattgtttat atcctttttg
      241 cagcaggtcc agacgagcca gcattgaagg aatgcatact tcatttaaca ggtcctgctc
      301 gagttttctc tgcttaatgg cgtcttcaat aaatgttttg tctccagtga taacgccaat
      361 ttcgaaacga agttcagacg tgctggcatt acatgataac ttttccatta tcgcgtcctc
      421 aacaatgaat tttgtgatgc ggtgcctggt gcctccaggt gacgttaacc agttaacaat
      481 taacgccgga tacagagcat tctcgttact cagcaaatga ccgctttacc gatttaactg
      541 ttccgcgtgc gctgagccgc attcaccgca tcacaaaatt cacttttaaa aaagggcggc
      601 agagcagtta cggagtaaaa ctgataccgc caaatgtcac cagaatattg ataacagagg
      661 gcgttgcagc ggggttgtca cttaagcgta tggtcaacct gacaacccgg tgtcctcaac
      721 ggggaaagaa taaccccgcc atacttaccg ccgcgccatt tcgcggattg ccacaaccgg
      781 aagcgcacgg tcgaattaaa tttaacgacg gctctaaggg agaaggactt cgccgtgcac
      841 tttcgtgtta tgccctgact tttcagggat atatcctttc agtaaactgt cagtgccgga
      901 ttcttatctg tgtccggcgc acgaccacac gtagcagcgt gttggtctcc atttttaatt
      961 cagcccttaa tggaggataa aatgccaaac aaaccatgcc ctgcctgtaa cgctctttct
     1021 ggctttttag agaagggcgg ttattacatt tttaactgtc cagaacatgt tgaatttcat
     1081 atatcaaaac ttgacagtat cattacaaag ccaaatcagt atcagtctga tttactgaat
     1141 aaagagttaa acgccgcccg agattaaggg cgtagtggcc cattaaaaat attgcaaaga
     1201 ccaactattg ttgaactgga ttaaaccagt aattctctat tgctcttatt gttaccatcg
     1261 gatgattgcc gggggttatt ttaagttcgg gaggaaaagg ctctccatca gcagccatca
     1321 ttgagtatgt gaaatccgcc gacgcagtcc ctttcatgat aattccgaat ttttcaagta
     1381 atgagagcag ttccggtaat cctgtgtcat ctgaataact ttcctcatag aaagaagcga
     1441 ttgccgcagg agatgacagt tctcgctctg caattttttt gtagttttcc agaattcttt
     1501 ttttgaatac ttttcctcga catgaaaacc catcaccgag gcaatccgct ttgcagtttc
     1561 ttcagtattc tggttgtttg ttaccgaaac agagtacaat ccggtaaacg catcgcgcac
     1621 attacgagcc atattatcaa tgtatttctt cgttgcagat tctaattcaa cttcgttcag
     1681 acgttggtga agtgcatgtg ctgcaatttc ctggatttct tttagtaaat ctttaaattc
     1741 catctttaac tccgttagtc gttagatttt gccgaactgg aaagcgcctg tttaaactca
     1801 ctgaagctga gagcttcctc gccttcggca aggccttcga agtattcttc gtaagccttt
     1861 tccatgattg tgtcgaaatc catatcactc acctgagttt ctttccagcc agcgacgggc
     1921 accattttcg gttttaaacg ttttgctttt ggtatacgtc atcgcggtga atgtaccgtc
     1981 ctggttggga aacacgccgt acaccagaga ttcgttgttg ccaagatcga tagtatccat
     2041 gttgacctca tttcccctta acgccgggta gcggaacaaa aacctgctgc atagttatta
     2101 aagttgaacc ctgccgtcat gttcatacgc ctcgggctgg ctacttaccc cctgaccact
     2161 gcttggtaac tcgaagtatt gcccggcgtt ctgtggggcg gggtgggtgg ttggtgtatg
     2221 taatctacaa taaaaaactg tttttgtgtc aacagttttt aattgttgtt ttgggcaaaa
     2281 aaatccctcg aaagagggag tatgaaaatt gttcaactca gatagagaag ggaaattgtc
     2341 gccgagaatg tgtcacgctt acaatctcaa tgcttgaagc agctactctg tacaggatta
     2401 tgtagttagg gtgggtcacg atctctctca atccagaaac ccgttcgctt ggtggatata
     2461 agtacggatg ctcagataga ggtaataccg atgtttcaat gcgtattttt agtctacgtg
     2521 ctgccggtgg gttctccttg gcgatgtaag ttatgatctg gcgcaaatca tcgcgagcag
     2581 acggtagcca taaaatgggt aacattactc gctcttgtta gttacagcaa tttgagcaat
     2641 aagattttcc atttcagcca ttacctcgtc atgtggaatt gcggggcgag tgtctgcaag
     2701 gcttgacgtt actttagtgc gcaaccattc gttgtaactg ttttcttgtt cggtagtttc
     2761 gaattctgaa actatcggag aaagggctgt acccatggca taactcctct tcttgtactg
     2821 tggtcacgcc cggcggcttt tttgtgccgc cagccaccta gcaatggttt cttccattga
     2881 ttttttcttg tctttgattt cttgaagcat tttttcttgg tcttcctcag gaaacgcact
     2941 aaaagcttgg agcagttcgc gttggcgagg accaattttc atcgtgtcag gggtgaaaat
     3001 ttgctcacac tcttcaggag gcaataaaaa ccaatgcaat ggatgccctg aaacctcaac
     3061 cagtttatcc aaacttgagg ctttaggtgt agccttaccg ctgacccatt gttgaacagt
     3121 ttgttgtgtc acaccaattc tacgggcaag ctcagcctgg ctccatccag tttcctgaag
     3181 aagcttgctg attctgtaca tagatacttc tagggcgctc atcattattc aattttacag
     3241 gtaaatactg ttaaaagcat cacaataaaa aactgttgat tgcatacagt tttttattgt
     3301 aggctttgct tatagttttt agaggagggc aaaatgctag atagcactcg cgaaaaaatt
     3361 aggcagaaat acactcaggc tgaaataggt cgttatatgg gggtcgctca acagactgtt
     3421 tggcaatggt ttagctttgg cgttccccca aagcaggtaa ttccgttatg ccaactaatg
     3481 aagtgggaag ttaccccgca tgaaatccgc ccagatattt atcctaaccc aaccgacggt
     3541 ttacctgttg gatgtaaggt taacacatca aatgcgccgg agttgattca tgaaaatcaa
     3601 gcatgaacac atccgcatgg cgatgaatgc ctgggcgcat ccggacggcg aaaaagtacc
     3661 ggctgcgaaa attaccaaag cgtatttcga gctgggaatg acgttcccgg aactgtatga
     3721 cgacagccat ccggaagcca tggctcgcaa tactcagaaa attttccgct gggtggagaa
     3781 agacactcct gatgcggtta aaaaaattca ggcgttgtta ccagcgatcg aaaaagcgat
     3841 gccgcctccg ctggtggccc gaatgcgcag ccacagttcc gcttattttc gggagttggt
     3901 agagacgaag gaacggctgg tgaaagatat tgatgatttc gttgcatcag cgatcgttct
     3961 gttcgatcag atgaatcgtg gtggcccggc aggaaacact ctggctgtgc attaattggg
     4021 taataaatat gagtaatgac aaaaaattga cactgagcgt ttacgaaaac agtccgcaca
     4081 tctggcgtgg cggtttatct gatgtggagc tggcagagtg gttgatacat aaagctaatg
     4141 cgctgctctg gcgtttgtca gccagagaac agcgcaagga aaccagaata aagctggctg
     4201 atgcagaagc gtgtgccggg cttattgagg attatacaaa tcttggtatt tcttcagcag
     4261 agagtgatcc cattcagcct ctgagcaggg agtcaatcca gcacgctggt tgtatggcac
     4321 atcttgtaac tgctcgtcaa catgaggtgg gtattggatc acttccggcg ggatattcgc
     4381 tgattccaga gctggttgaa gcaagaaaat cagttcagaa aaagagagat gacgcacttc
     4441 aattattgag agagcactat ggcgcgatac cagaatgcga acagcgtcga taccctgaag
     4501 gttatgaatg gatgcagtct ctttttgaag ttcgctaatc aatatgtcga gacgaaggta
     4561 tgtttcggcg cgcagccagg ctctgtaatc cgggagcatt tcggggctgt tacaccagcg
     4621 gtttgttgct gcaacattta atacatgagc ctgataaagg cttttcaaaa aatacatgtc
     4681 gaacctcctc tggttctgtc gattgggaac cacagattat atccggagga aggttcggca
     4741 ccagatgagg tagccatgcg tgattacgca aaagtttctc cgcgattctg gctgggagaa
     4801 acggggagag aacttagaaa ggcgggtgca gaagcgcaag ttgttgcttt ttacctgatg
     4861 acatcccctc acgcaaatat gctgggtttg tattacctgc cagttttata ccttgctcat
     4921 gaaaccgggc ttggtctgga aggggcttca aaggggctta aaagggctgt tgaagctggt
     4981 ttttgtagct atgaccatga tgcagagatg gtctgggtcc atgaaatggc agcctggcag
     5041 gttggggaaa cgttgaagcc tggcgataac cgttgtgcag gtgtcaggaa tgagtatgca
     5101 tcattacctg aaaacgcttt tctgtcagtg ttttacgaca gatataaaac ggatttccat
     5161 ctggatgtga ggcggaataa tagccgaaat tcggtaaggg gcttcgaagg ggcttttaag
     5221 gggcttcgaa gccaagaaca ggaacaggag caggagaaag aacaggaaca ggacaaaaac
     5281 actatggttc atggcaaaaa aaacaccacg aaccaggcag gggatgttca gaccgtcaat
     5341 cctggtcagc cagcaggcac gacaccggaa gccgattcgg gcgctgtgca gcaggtgatg
     5401 accgcagggt cggagcaatc acaccaactg cagcagcctg aagccgattc cgccattcag
     5461 cgggaagccg atcgggtagt cccggaaagc accgggcagt ctgtgggacg agtggattat
     5521 ccggatgtgt tcgaacaggt ctggcgggaa tacccgttgc gtgctggggc aaacccgaag
     5581 aaatccgctt tcagtgcctg gaaggccaga ttgcgcgagg gggtgccacc agagaccatg
     5641 ctggatggtg tgaggcgtta cgcgagatac ctggcggcga ccgggaaagc gggaacggaa
     5701 tttgttcagc gagcgacgac gttttttggg ccggaccgga attttgaaaa cccctggttg
     5761 ctcccggtaa gcggcacgaa caaccagcgt tgtgtgaatc atatttctga accggatacc
     5821 gaaattccgc cgggattcag ggggtgatgt ggcatgaaaa acattgcggc agccggggtt
     5881 cttgaacgta ttcgcagact tgcaccacag gcgtcggttc caccgtaccg gacggtggag
     5941 gagtggcggg aatggcaact tgctgaagga cgaaaacgca gcgaggagat taaccgccag
     6001 aatcaccagt tgcgggtgga aaaaatcctg aatcgttcgg gcatccagcc tctgcacagc
     6061 aaatgctcgt ttgcgaatta tcaggtgcag aacgacgggc aaaaatacgc gctgagccag
     6121 gccaaatcca tagctgacga actgatgacc gggtgcacga attttgtgtt cagcggtaaa
     6181 accggcaccg ggaaaaatca ccttgcagcg gcgatgggta accggctgat ggcgaagggg
     6241 cgcagcgtga ttatcgtcac cgtgtctgat gtcatgagcg tgttgcatga cagctacgac
     6301 aacggcaaat ccggggaaaa atttttacag gagctttgca gtgttgattt gctggtcctg
     6361 gatgaaatag gcgttcagcg ggagacgaaa aacgagcagg tggtattaca ccagataatt
     6421 gatcgccgga cagcatcact gtgcagtgtc gggatgttaa caaacctgaa tcatgccgca
     6481 atgagtacac ttcttggtga gaggattatg gaccgcatga ccatgaacgg tggtcgatgg
     6541 gtgacgttta actgggatag ctggcgtcca aatgtcagca atatgagggt tgtgaagtaa
     6601 ttttgtccgg aggaaatttt aatggaaacc gtatctgacg cactgaaagc actgaaaaaa
     6661 gcctcttcac atgtggtggc agctcgcctt ggaatcagtc gtgaagaggc tgtcaacgag
     6721 ctgtgggaac tcaaaataaa tggcgtcgtt gataaaactg gtcacacctg gtttctggct
     6781 ggcgaaggtg aatcccgggt aaccgaagag cggccagtaa aatctgaagc acaggatatg
     6841 ctgaccgggg aggtcgaaca aaaagttacc gcagacatga tgattgagtt tatcggtcag
     6901 gatggggcta aaacgtgtga ggaactggcg ggtaagttcg gtgtcagtac tcgcaaggtt
     6961 gcttccacgc tggcggtggt aaccgcaacg gggcggctgg cacgcgttaa tcagaacggt
     7021 aaatttcgtt actgcatgcc gggcgataat ttaccagcag agccgaaagc cgcgctggta
     7081 acggaaagtg atggtaaggc ctttcctcag ccagcaggtg ctgcgttacc agtccgggaa
     7141 gccgcaacac aggaagaaat taaaacagaa actgtggcgg acattgtgca gccgttgcca
     7201 tcgtttaccg aaacgcaagc agatgagctg atttttccgt cccttcgcag ggcaaacctg
     7261 gcgctgcgca gggcgaaaag tgatgttcag aagtgggagc gagtctgcgc cgcgctgcgg
     7321 gagctgaaca agcaccggga tattgttcga cagattactg attcttcccg ccgtgttgta
     7381 tcggaaaagt gattgccgga ggcgcttatg gcaaaagtat ttacacagga agagcgggaa
     7441 aaaattaaag ggcaggttgt tgagctagta cgccggagtg ggcgcgagac gttacggcaa
     7501 ctggaagcca agacaggtgc gacaagatat ctgatgagcg ttcttgccag agagctggtt
     7561 gccagtggcg atgtatacaa ctctggttac gggttattcc catctgaaca ggctcgtaag
     7621 gactggcaaa acgcccgcaa aaaactatcg agggcaaaac tgaagaaacc ggttgtggtt
     7681 gatcctgacc ttatctggtc attaccagac ggagaaatac gccgctatga caggcaattg
     7741 aacataattt gtagtgagtg ccgtaacagt gaagtgatgc agcgagtttt gatattttac
     7801 acaggagtaa tgatggaata gtgaaaataa catgaaacct ttggattctg gtgtttcagt
     7861 ggatgggaaa agagcagata catagaaaat gaatagcaat aattcacagt ctggatgttg
     7921 tttgtatgct tataaaatga tcctagtatg cattgccgga gttgaatcag ctccaatgaa
     7981 gaaactgaga ggagtattta gaagatagct aaatggatgt aattattagt atctaaaaaa
     8041 tagtattttt gaaatgggtc t
//
LOCUS       DAZKIF010000169         8013 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZKIF010000169 DAZKIF010000000
VERSION     DAZKIF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.170"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(178..1035)
                     /gene="murI"
                     /locus_tag="ACHOTW_004187"
     CDS             complement(178..1035)
                     /gene="murI"
                     /locus_tag="ACHOTW_004187"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9846191.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(980..2824)
                     /gene="btuB"
                     /locus_tag="ACHOTW_004188"
     CDS             complement(980..2824)
                     /gene="btuB"
                     /locus_tag="ACHOTW_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP9846192.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2889..3079)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3193..4293
                     /gene="trmA"
                     /locus_tag="ACHOTW_004189"
     CDS             3193..4293
                     /gene="trmA"
                     /locus_tag="ACHOTW_004189"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP9846193.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4333..4692)
                     /gene="yijD"
                     /locus_tag="ACHOTW_004190"
     CDS             complement(4333..4692)
                     /gene="yijD"
                     /locus_tag="ACHOTW_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP9846194.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4692..5342)
                     /gene="fabR"
                     /locus_tag="ACHOTW_004191"
     CDS             complement(4692..5342)
                     /gene="fabR"
                     /locus_tag="ACHOTW_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP9846195.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5446..6762)
                     /locus_tag="ACHOTW_004192"
     CDS             complement(5446..6762)
                     /locus_tag="ACHOTW_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFP9846196.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6805..7971)
                     /locus_tag="ACHOTW_004193"
     CDS             complement(6805..7971)
                     /locus_tag="ACHOTW_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFP9846197.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2096 a         2179 c         1900 g         1838 t
ORIGIN      
        1 gctacagagt caagcattta tttttgcttt ctctgtcgga attctcagga gaaccccgct
       61 gacccggcgg cgtgtttgcc gttgttccgt gtcagtggtg gcgcattata gggagttatt
      121 ccggcctgac aagaggaaat ttaaaataat tttccgaccg cgcaacattc aaccaaatca
      181 gcctaaaact gccagttttt cgagcgtttc gaagccgtaa cgctgtaaaa cgggcaataa
      241 ttgttcagct tctggcgtca ttgccataca aaaggcaata ttcgcatcgg cagattttgc
      301 atccggggct tcatgttcta acaaccaggc cgttcggcga gcaatcgctg cgccagaatc
      361 caccagccgg gttccctctg gcagcacttg taacagttct tcttgtagta gagggaaatg
      421 ggtgcaaccc aatacaacgg tatctggcgg ctctttcatt cttaaccacg ggcgtaggat
      481 acgttttagt gcatccagag aaacatcttc gccatgcagc ttcgcttcag ccaactcaac
      541 catctctgcc gagcccagca tttctatctg gcattcatta gcgaaacgcg cgatcagctc
      601 atgagtataa gaacgtttaa ctgttccgcg ggttgccagt aatccgacaa tgccatttgc
      661 cgtcagacgt gcagcaggtt taatcgccgg cacgacacca acaaccggga agtcgaactt
      721 ttcgcgtaat gcaggaagtg aaacggtact ggcagtgttg caagcgacca cagccagcgc
      781 aaggggataa cgttcttgca ccgcggtgac aattgccacc actcgctcaa caataaacgc
      841 ttcgcttttt tcgccatacg ggaaagcgac gttatcgaaa gcataaatgt aatggagatc
      901 cggtaagaga tgccggatct cgtcatagac cgacaaccca ccgacgccgg agtcaaacac
      961 cagcacggtg ggacgtggtt cagaaggtgt agctgccaga caaggtgtat tcccgtcctg
     1021 cagtttggta gccatagact gtctcataat ctttgtcgaa caggttggct attttaccac
     1081 gaactgtcag gtgagaggtg accggatacg caaccgcaag atcccacaag ctcacaccgc
     1141 ccattttaac ggtttgataa ggataagatg agtaatcctt atcatagcga gtgcctaaat
     1201 actgataagt aataccccag tcgaagtcat acaactgcca gtcgagctgg tatttcacct
     1261 gctgtttagc acggcgtaac aacggcgtgt cggtaattgc attgcgcgca tcgacataat
     1321 cataactcac agtatgcgtc agtggtccgg tatcaaaatt ggcggtcgcc tcgacgccct
     1381 taatccgcgc tttcccttcg ttgtaatatt tcagggtgtg atcatcataa tcgatcaagt
     1441 cactgacatc gttacgatat ccggaaatac gccagttcac cccagcggtt aagccttcaa
     1501 acgcgccttc ccactgtttg cttttctccg ggtccagatt cggatttccg tagaagccat
     1561 acagttgccc cagatttggt gccttataag atgtcccgta ggaagcaatg aagcgataac
     1621 cttcgatgaa ttcccaaccg gcgctggttt gccaggttcc atgacgacca aactgtgagt
     1681 tatcgtcgct gcgtgctgcg ccttcaaagg taaaatcgcc gacttgttgc agcccggtca
     1741 gatagatgcc ggtattacgt tgatcatatc catccttaac ataagctgtg cccggtgccg
     1801 tgctctgctt ctgccagtca acacccgcac caacattacc gtggccaatg atgatgttgt
     1861 ttgcccactg gacggtgtat tgcttcatct catcgagcgt cgccgacgaa tcataacgac
     1921 cataatgggg atcgtagttg taatctttgc tatggctata gctggtaatg agttgtgatt
     1981 taatcagttc gccgttatat cgcagcccgg cgtcccaact ttgactatag agtttacggg
     2041 tatcgaccaa tggtgaaccc ggagaataat acgcgtcata attggtacgg ttatcatagc
     2101 catagccgcg cacaaagccg ctccaggcat cagtaaagtt atgctccagc gcgccataaa
     2161 gcgttttact taaaaaacca tcgttatctg gctgcgcttg cgttccggta ttaccatagg
     2221 caacaacatc ataaccatga gtatgggcat aatcgcccaa caacgttact cgtgtcttat
     2281 cccccagttg ttgctgtgta gagacatcat agttttgata actattgctt ccccaccctg
     2341 ctgaaatttc cgttccgggt tcatcgcgcg tcgtgatgat attcaccacc ccacctattg
     2401 catcggaacc ataaacggcg gagcgtggcc cacggatata ttcaacacgc tggacaagcg
     2461 caatagggaa ctggctaagg tcggcagaac cactcccccc cgccagattc aggcgtacgc
     2521 catcaattaa caccaacaca tgactggcat ttgtaccgcg aataaaaata gatgagagct
     2581 gacctgaacc gccgttttgg gtgatatcga cgcccggaag acggcgcagc acatcattaa
     2641 ccgaggtcga ctgccagcgg tcgatatcct gacgcgtcac aacggtggtt ggtgcaagca
     2701 cagtgctgcg cggctgttca aaacggttag cagtaacgac gagagtatcc gggctggtat
     2761 cctgtgccca agcggaaaag gctgtgacag aacacgccgt cagcagcgaa gcttttttaa
     2821 tcattgtaaa gcatccacaa tagaagaagg atgccgcagg tttcatcaat attacgcgat
     2881 gatgagaacc agatgcgacg ttggccggca ggtcttcggg cttggagggg tatctaagat
     2941 actaagagat gatgacttcc caccgaatgg cagtgtccgc ataacgcaat catcgcacct
     3001 ttccttaccg ctgcgcgtca gctccagatt cgcactggat tccctattaa ctcacaggac
     3061 cggcaagtgg atgctacagg ttgtaacaag ttactgtcca gacgtagctc acaaatagga
     3121 attcatcaag atctggacat ctgatgagca atccctacaa tcgccgcgta ctttaatttt
     3181 tcaggataca tcatgacccc cgaacacctt ccaacagaac agtatgaagc gcagttagcc
     3241 gaaaaagtgg tacgtttgca aagtatgatg gcaccgtttt ctgacctggt tccggaagtg
     3301 tttcgctcgc cggtcagtca ttaccggatg cgtgcggagt tccgcatctg gcacgatggc
     3361 gatgacctgt accacatcat tttcgatcaa caaaccaaaa gccgtatccg ggtggatagc
     3421 ttccccgccg ccagtgaact catcaaccag ttgatgacgg cgatgattgc gggtgtgcgt
     3481 aataatcccg ttctgcgcca caagttgttc cagattgatt acctcaccac gctgagtaat
     3541 caggcggtgg tttccctgct gtaccataaa aagctggatg atgagtggcg tcaggaagcg
     3601 gaagccctgc gcgatgcact gcgcgcgcag aatctgaatg tgcatctgat tggtcgggca
     3661 acgaaaacca aaatcgcgct ggatcaagat tacatcgacg agcgtctgcc ggtcgcaggg
     3721 aaagagatga tctatcgtca ggtagaaaac agctttaccc agccgaacgc ggcgatgaat
     3781 attcagatgc tggaatgggc gctggacgta accaaaggct caaaaggcga tttactggag
     3841 ctgtactgcg gcaacggtaa cttctcatta gcgctggcgc gcaattttga tcgggtatta
     3901 gccaccgaaa tcgccaagcc atcagtcgct gccgcgcaat acaacattgc cgctaaccat
     3961 attgataacg tacaaattat tcgtatggcg gcagaagaat ttactcaggc gatgaatggc
     4021 gtgcgcgagt ttaaccgcct gcaagggatc gacttaaaga gttatcagtg cgaaaccatt
     4081 tttgtcgacc cacctcgcag cggtctggac agtgaaaccg agaaaatggt gcaggcgtat
     4141 ccacgtattc tgtatatctc ctgcaatccg gaaacgttat gcaaaaatct ggaaacatta
     4201 agccagacgc acaaggtcga acgtctggct ctgtttgatc agttccccta cacgcaccat
     4261 atggagtgcg gcgtattact gaccgcgaag taaaacccat gccggatgcg ccagcatccg
     4321 gcatgatacc gattactcag caacttcctg cttacggtta cgcatcttcg cgccaatcca
     4381 gaacaccata atgacggaga gtactgccgg gaagaagtta gagccgatat ccggatattc
     4441 cgcacgaact accgtgctgt acagcagtac gccgagaata aaacaggcag ctgccagacc
     4501 cggcaagcct accggcatgg tgcgattaag ataacgttga tgcaggcagt aaaccgtcag
     4561 caccagggaa ataatcggga atacagaaaa tggcacaatg gagctaaaca gcgctgcgaa
     4621 agtaccatta atcgataagc cagcaaccaa cgccagcagc agcgtacctc tatcttgatt
     4681 tgcttgtttc attactcgtc cttcacattt cccggaataa ttgcggtttt ctcttgttca
     4741 cggcgatacc agtaataagc ccctttcgaa atcattcgca gttgcagtac cagtcgctct
     4801 tctaattgcc gacgttgttc aacgccgacg tccaacgcct cggcacccgc actgaagaca
     4861 attgtcacca ttgcttcggc ttgcgcttca gtaaacgcac gcggcatatg gttttcgagt
     4921 tccagatagt ccgcaagttc cgcaatgaag tgctgaattt cacgcgcaac ggcggcacga
     4981 aacgcagcgg aggtgccgga gcgttcccgc aataataacc ggaaggcgtt aggattatta
     5041 ccgatgaact ccataaatgt ggagaccgag gtgcggatca cactcccgcc tttggcgata
     5101 cgctgacgcg cctggcgcat gagttggcgt agcattaaac cgctctcatc aaccatggtc
     5161 agacccagtt cgtctacgtc tcggaaatgc cgataaaaag aggtgggagc aatgcccgct
     5221 tcacgcgcca cttcacgcaa actcaggctg gcgaagctgc gttcagcact taattggcta
     5281 aatgcggctt ccaccagcga acggcgggtt ttttcttttt gttgcgctct tacgcccatc
     5341 acgatgtctg aatccttgcc aaaagtactg ctggcactat accagagaat gaacatactg
     5401 gattgctcca gtattgtgaa tgaacggtaa cgcaataaaa catatttact gcaattcttt
     5461 ggccggttct tttacgtaca acaacgccaa catcgacagc aacgtgcctc cacccagata
     5521 ccacgccgga gccgtcaaac tatccagcaa cacgatggat tgcgtagcga aatattgagc
     5581 aaaaccgcca aagattgcca caccaatgct ataaaccagc gcaaaaccca atgcccgaat
     5641 ccgtttcggc aacagttcag agatcaacaa catcactggc acaccgccaa gcgtggtaaa
     5701 gctgaccatc acgaaaacca ccgtcagcaa catgcctggg cttgggtaat tcaccaacag
     5761 ccagaatgac ggccagctac agatcagcac catcacacgg gagattaaaa tcagcttttt
     5821 acgcccgacc gaatcgcaca acatccccac cagcagcgca cccacaaacg taataacgcc
     5881 cgccagtaac atcgctgcat gagaatagtt ttggttcatt cctaaatact tcgccgccca
     5941 tgtaccgtaa taaaagaggg agatataggt tgcgactgta ctaccaatag cgagcaagat
     6001 accgttagcg atagtcgctt tatgttgcat aagtaaggag aaggcgcttt cgctggctgc
     6061 ggccttctta ttatgtgctg gctcaggaac atcattttcc agactcaaac gcaaccaaca
     6121 gccgacaggc gctaataaca cgccaataaa aaacggcaca cgccagcccc attcagccat
     6181 gacggtttct gaaccggtcg caaagggcag ccatgcactt aaacctaaag cgacaacacc
     6241 tccaaaagtt gttgccaggc cttgagtcgc cagtgaccaa ctgctgtaaa aaccgcgacg
     6301 atttgccgga gctgattcaa ccaacaaaga cattgaggca ccgacttcgc ctcctgccgc
     6361 aacgccctga attagacgcg ctagcaccag cgtcgcggtt ccccaatagc ccgccgtttc
     6421 atacgtcggt gcgatgccga tcatcagtgt accaagactc atcagcgcaa tggtgaacac
     6481 catcgcgggt ttgcgtccga aacgatcggc ccaggccccc acaataatgc cgccgagcgg
     6541 gcgcataaaa aagctcacgc cgaaagtggc aaacgccagc agtagcgcga cgtttttatc
     6601 tgcactgggc ggaaaaaata gcgtgctgat atagaccaca aaaaagttgt aaacgccaaa
     6661 atcaaaaaat tcgagcgcat tgccaaacgc cgaggcaaaa atcacccgcc tgccgctggg
     6721 ctttttcttc acactcacgg tttcatttac gatcgccgtc attccatgtt ccttgccaaa
     6781 aagtgttgtt gtgtgttgtt ttatttattg cagccaggct tctaccagtg cgccccagta
     6841 gctgcttgcc gggaccagac ttgcatcgtt gaagtcgtaa cccgggttat gcaccatgca
     6901 ggaaccttcc ccgacatcgc cattaccgaa taacaggaaa ccgcctttgg gttgggcttc
     6961 cagcatgaaa gagaaatctt cactgcccat caggggcttg atgccaaact cggcccgatc
     7021 ttcaccaaac gttttacacg ccacctgcca ggcgaagcgc gccatctcct cgtcatttac
     7081 taatactggt gtaccgttaa catgcgtaac ctcagccgta gcgccaaagc tggcagcctg
     7141 ggcttgtgcc agagcaggaa tacgggtcag aagaagttgt cgggtatcgc ggctaagaga
     7201 gcggacgctg attttcatct cagcactgtc aggaatgacg ttagccgcct cacccgcaac
     7261 aatgctgccg acggtaatta ccgccgcttc cagcggatcg acattgcgtg acacaatgct
     7321 ttgtaatgcg gtggtgatat gtgcggcaac tagcaccgga tcgatggctt tgtgcgggat
     7381 cgcaccgtgt ccgccacaac cacgaactgt aatatggaac tgatccattg acgccatcag
     7441 tgcccctggc tggaaataaa acttacccac cggcagaccg ggcatgttat gcatgccgaa
     7501 aatgacatcg caggggaaat gatcaaaaag cccctcttta accatgatct caccgccatt
     7561 aatcatctct tccgcaggct ggaagataag acgtaacgtg ccgttaaaac ggcgggtttc
     7621 ggcaaaataa cgcgcggccc ccagcagtat ggtggtatgc ccgtcatgcc cgcaggcgtg
     7681 cattaaacca ggatgtttac tggcccaggg cttgccacta ttttcataaa tcggcagcgc
     7741 atccatatcc gcccgcaggc caatactttt ctctccgtcg cccactttta acgtggccac
     7801 tatccccgtc gtcgccagtc cttgctctac ttcatagccc cactgacgca gcttttccgc
     7861 aaccaaagca ctggttttaa attcttgtaa tcccagctcc gggttttcgt ggatctgatg
     7921 gcggatctca cgcagttctt cctcaaaccc tcgaatatag tgttcgatca tcggctattc
     7981 ctttattcac ttataaacag gctaattaac gga
//
LOCUS       DAZKIF010000170         8010 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZKIF010000170 DAZKIF010000000
VERSION     DAZKIF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.171"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(539..679)
                     /locus_tag="ACHOTW_004194"
     CDS             complement(539..679)
                     /locus_tag="ACHOTW_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9846198.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(981..1244)
                     /locus_tag="ACHOTW_004195"
     CDS             complement(981..1244)
                     /locus_tag="ACHOTW_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9846199.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            1669..1794
                     /locus_tag="ACHOTW_004196"
                     /pseudo
     CDS             1669..1794
                     /locus_tag="ACHOTW_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            1859..2119
                     /locus_tag="ACHOTW_004197"
                     /pseudo
     CDS             1859..2119
                     /locus_tag="ACHOTW_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2169..2408
                     /locus_tag="ACHOTW_004198"
                     /pseudo
     CDS             2169..2408
                     /locus_tag="ACHOTW_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2456..3073)
                     /gene="ureG"
                     /locus_tag="ACHOTW_004199"
     CDS             complement(2456..3073)
                     /gene="ureG"
                     /locus_tag="ACHOTW_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFP9846200.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(3085..3759)
                     /locus_tag="ACHOTW_004200"
     CDS             complement(3085..3759)
                     /locus_tag="ACHOTW_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFP9846201.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(3760..4224)
                     /gene="ureE"
                     /locus_tag="ACHOTW_004201"
     CDS             complement(3760..4224)
                     /gene="ureE"
                     /locus_tag="ACHOTW_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFP9846202.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(4234..5937)
                     /gene="ureC"
                     /locus_tag="ACHOTW_004202"
     CDS             complement(4234..5937)
                     /gene="ureC"
                     /locus_tag="ACHOTW_004202"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFP9846203.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(5930..6250)
                     /locus_tag="ACHOTW_004203"
     CDS             complement(5930..6250)
                     /locus_tag="ACHOTW_004203"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFP9846204.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(6259..6561)
                     /locus_tag="ACHOTW_004204"
     CDS             complement(6259..6561)
                     /locus_tag="ACHOTW_004204"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFP9846205.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(6652..7350)
                     /locus_tag="ACHOTW_004205"
     CDS             complement(6652..7350)
                     /locus_tag="ACHOTW_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFP9846206.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            7731..8006
                     /locus_tag="ACHOTW_004206"
     CDS             7731..8006
                     /locus_tag="ACHOTW_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846207.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1937 a         2045 c         2030 g         1998 t
ORIGIN      
        1 attatcgttt tattctttca cttttatcaa aacatcacca gaaactggaa gtgacaaccg
       61 caataatgaa cataaaaagg caaatcagaa tggcagtaaa tcaataaagt accatttcat
      121 atgaatcatc agaaaaacgg tggacgcgac atccagcacg aggcaaaaca cagcaacaaa
      181 ataagccgga tatcgcgccc tgtacttttc taactcaact cccggcattg tgcaagtttt
      241 aatcgcaggt ggattcagag ccacgccccc attgattcag cctgaagaca cgtatatcct
      301 tggagaaagc aggaatctca ttgctgtgaa attccttaca gaatcttcag ccccatttta
      361 tggggctttt tttggtgccc ccacgctgtt accacacttt cgggatgcga ataaaaaagc
      421 ccacagtttt tacactgcgg acttttgttt tttctttgtg atttgtgtgc aagggtgcga
      481 ctttcgaccg aacctttggc aaccggatag cggggactgt tgtccccgct gcggtttctt
      541 acggtttaca ctgcaagaac gccgcaattt ccgaccacat gaagcttacc ctgaattcac
      601 acggtgatgc gtgtaacacc cagacggtca gcaacatggt caggcataac atgcccacaa
      661 tgatcgtttt ttgcggcata gcacttgctc cttttacaga gaggcgctaa ccttccactt
      721 gctgaaggta gatggtcagg gcctcgggtt aacgtttaag ttgactcggg gcctttccac
      781 atccggcctt caggtaatcc ttccagccgt cagccgaaag gcacccgcgc gtaatgtact
      841 ggttttcaga gaggtccgca atcttccggt gacgagatca tctgcacacg ccccggtggg
      901 acgggctaac ggtatattca ggtgcaggat ctctctgtct ggtgcgtttt taaattgtca
      961 aacgcaccct ggcggcaaaa tcagccacct tttttcgcat caacaaaacg cccaaaacgc
     1021 tgggtaaagc gtgcaacatt accttctgaa gcgacagttc tcagtttacc tgtataaaac
     1081 ggatgcgact tagaagaaac gtcaattgta acatatgggt aagttacacc atccagttca
     1141 attacccggt cagttttgat cgtcgaaccg atcctgaagt actcatcaat actggtatcg
     1201 tgaaatacta ctgtacggta ttccggatgg atattcggtt tcacaacagt tttccccctt
     1261 taagtgaaaa ttccgcataa aggtacatat tcagacggtc agattcaact gtctgttctt
     1321 ataacctcac tctctttcag tattacaata tgttatataa ttataaactg gacttcccga
     1381 aaaaatccat taaataagaa gcgagccgtt gcggaggctt gctctctcct ttaacctcat
     1441 gtgcaatatc cgtaacgcgc tcacataaat catcatctga taatataaca acacccatta
     1501 ttttattctg acctaaaaac ttcttatgaa ataaacgttt acccgacgta tctgatcccg
     1561 ctgtttttac ctgtatcctt taacatactt gagaagcccg ctgaaaaaat ataccgtaaa
     1621 ggcggtttct ctgtcttcca taaacctgtc ggataagaag ggcattaaat gaaaaccaaa
     1681 aaagttatga tgattgcact ggtcagctct gcgctggctc tttcaggatg tggagccatg
     1741 ggcacagcga tcaaaaaacg aaatctggaa gtaaaaaccc agatgagtga aacctgaggt
     1801 agcctgagtt taacggacac tccttcctga aatagaatgg catcagaagg agctaataat
     1861 gagcagaaaa acccaacgtt actctaaaga gttcaaagcc gaagctgtca gaacggttct
     1921 tgaaaatcaa ctttcgatca gtgaaggcgc ttcccgatta tctcttcctg aaggcacttt
     1981 aggacaatgg gttaccgccg ccagaaaagg gctcggtact cctggttccc gcacggtggc
     2041 tgaactggaa tctgaaattc tgcaactgcg taaggcgtta aatgaagctc gccttgagcg
     2101 agatatatta aaaaaagcag ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta
     2161 tctctttcac tgcccgcgac ggtatcggtt atcagtttga cctgctggct aacggtgaaa
     2221 ccgtactgcg tgatgcatcc ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc
     2281 tcatgaatgt tggcctgaat gcggaaattc gcgacaacat ccgcttcggt ctggagtttg
     2341 aaaaatcggc atctggtaag tacaacgtgg ataacgcggt gaacgccaac ttccgttatt
     2401 ccttctgata aaaacaaggg gccgcctgcc ggccctgata cagaggatgt tgtgattact
     2461 tacccagcat gccctgctct tcgataaagg cgatgatgtt ttgcagacct tcaccacttt
     2521 tcaggttagt gaacccccat gggcgttctc cgcgcatacg taaagtatca cgctccatta
     2581 cctcgaggga ggcgcccacg tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg
     2641 atttggtgat ccccggaccg cctttacgcg ggatcttttc tccttctgcc acatctatga
     2701 catatatggt aaggtccgcc agttcggggc tgaaggtcgc actcaggttg tcgccgccgc
     2761 tttccacaaa gatcagctcg agattaccaa atttctcact cagtgcttcc acggctgcca
     2821 gattcattga tgcgtcttca cgaatggctg tatgagggca gccccccgtt tcgacaccga
     2881 caatgcgttc cgcttccagt gctccggcct cagtgagaat gcgttgatct tctctggtgt
     2941 agatgtcgtt ggtgactacg gctaattgcc aggtgtcacg catggatttg cagagggctt
     3001 ccagaagcgc ggttttacct gagccgaccg gaccgccgac gccgacacgc agcggttgct
     3061 tgattatgtt cattttattt tctcctaaga acggaataat cgggaatact gggtttcatg
     3121 tcgcgcggaa gcgatagatg caagtggcgt tgctgaacca attgcgtgat ccggggttgc
     3181 aagggcactg tccatgttcg ccgcatagcg ggcacacagg cgcataatta actgctgggc
     3241 agccagctgg ccgtagggta ccagcctgac gcccgccatc actgcgcttt caatccagct
     3301 gtagccaagg ctcagggcca ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc
     3361 cataccggtt aactggcttt gctggcatag tttgcgccat tcggccgggc agtcgggctg
     3421 ccagtcgacc agaagtctgg tgaaggctgc accgcggtta cgttcttcat cgcgtagctc
     3481 acgggtttcc ctgcaggcca gcagataagc ggtccagcgc ccggcactgt caggatcgcc
     3541 cgcttcacag gcccggtata gccgggcaag cagtggcagg tcgacggcaa aaaaactctg
     3601 ttccatctgc aactgctgcc agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc
     3661 cactgcccac tccagtccct gggaccagct gtagcctcca acagggaggc tgttactggc
     3721 aagctgcatc agccgcagac gtttttccgg agtcggcatt cagtggctgt gctcctgatc
     3781 gtgattatgg gcgtgcgact tgctggcgta agcgccggct tccggctcaa acggaagatg
     3841 tgcaaaatca acatccagcc caaactggcg cagcatatca tcgaggacgt gatcgtgatg
     3901 gtagcgcagc tctccgggca ttatttgcag cggaacgtgg cgattgccga gatgccagca
     3961 ggctttcgcg agcataaaag gatctgcgca gcgcactaca gaaacggctt catcagcggc
     4021 gatcacctta atgaattcat caccgttttc gttactgaga atatcaccat cgcgcagcag
     4081 cagaccgcgt ggcagcaaca gtccagcctg acgtccatcg ttaagggtga ctttaatacg
     4141 gctttttacc cgcatgtcaa cagggagggt aacgctggca gttgtctgag ccggggtctc
     4201 cacccgccgg gtcaaataaa gcatttgtgc tccttaaaac aggaaataac gttgcgccat
     4261 tggcagaatg tccgccggtt cactggttat cagttcgccg ttgatacgca cttcgtaggt
     4321 ttgtgaatcc acggttatat caggcaacag gctgttgtgg cgcatatcgg ctttttgtac
     4381 tgtgcggcag ccttttacca cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc
     4441 atttgctgct gctgcctgcg acaggaaagt cacacgacag cggtgacggg cactgcccaa
     4501 tgcagcgaac attgggcgat agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc
     4561 acccatcggc gccatggcta tcattccgcc tttgacgata gtcgccggtt ttacgccaaa
     4621 gaacgccggg gaccagagca ccagatccgc cagttttccc acttcaatcg agccgacttc
     4681 atgagcaata ccgtgggtta atgccggatt aatggtgtat ttagcgatat agcgcttcac
     4741 gcggacgttg tcgttatcac cactttcttc cggtaacggg ccgcgctgaa ctttcatccg
     4801 gtgtgccacc tgccaggttc gtaacactac ttctccgacg cgtcccatgg cctgcgaatc
     4861 ggacgaggtg agggagaacg cgccaaggtc gtgcaggacg tcttccgcgg caatggtttc
     4921 ctggcgaatg cgcgattcgg caaaggctac gtcctcggcg atatccgggt caagatgatg
     4981 gcaaaccatc agcatgtcca gatgctcatc aatagtgttg acggtatagg gtagcgtcgg
     5041 attggttgag gaaggcagaa tattggggtg cgcgcaggcg gtgataatat ccggagcatg
     5101 accaccacct gcaccttctg tatggaaggt atggatagtg cgcccgccga tggcagtcag
     5161 agtatcctca acgaatcctg attcgttaag cgtatcactg tgtagcgcaa cctgaacgtc
     5221 catttcgtcg gctacagtca gtgcgcagtt gattaccgca ggtgtagctc cccagtcttc
     5281 gtgaattttg aggccgataa ccccggccgc gacctgctca cgaagcgcat ccggattgga
     5341 gcaattgcct ttacccagca acccgatatt gaccggcagg ctgtctgcag cctgcagcat
     5401 ttgataaatg taccatggtc ctggggtaca ggttgtggcg ttagaacccg ctgtcgggcc
     5461 agtaccgcca ccgatcatgg tggtaatgcc ggatgtcagc gcttcttcag cctgctgtgg
     5521 gcagatccag tgaatatgcg tatcgacgcc acctgcggta acgatcctgc cttctgcggc
     5581 aataatttcc gtggatacgc cgattgggat agtgacgttg ggttgtatat caggattacc
     5641 ggctttgccg atagcaaata tccttccatc tttgacgccg atatcggctt taacgatccc
     5701 ccagtaatcg atgatcaggg cattggtcag caccagatca gcgcagccgg cggagagcat
     5761 ttgcccctgt cccataccgt cgcggattac tttaccgccg ccgaatttga cctcttcgcc
     5821 gtaggtagtt aaatcatctt cgacctcgat ccacagctca gtgtctgcca gacgaatttt
     5881 atcaccggtg gtagggccga acatgtcagc ataggcctgg cgtgaaatat tactcatcat
     5941 ttgcctccag ctcacccatt atatctccgc ggaaaccgtg cacgatacgg gcaccgctca
     6001 gcgccaccag ttcaacctca cgcttttggc ctggttcgaa gcgcactgca gttccggcgg
     6061 cgatattgag acgatagcct cttgccttct ggcgatcgaa cttcagtgcc gggttaacct
     6121 ccgcgaagtg ataatgggaa ccgacctgaa ttggtcggtc tccgtgattt tcaacgataa
     6181 tactgcaggt agcccggccg atgttaagct ctatatagcc aggtttaacc ttatattctc
     6241 cagggatcat ctgttacctc agattatcgg attatggacg gtaaccagct tggagccgtc
     6301 cggaaaggtg gcttcgacct ggatatcggg gatcatttct ggtatgcctt ccatgacctg
     6361 ctcgcgactc aggacatgcc gtccttcttc catcagcgca gcgacgcttt tgccgtcgcg
     6421 agcgccctcc attataaaag cgctaatcag ggccacggat tcgggatagt taagtttcag
     6481 gccgcgggcc agacgacgct ctgccagcag tgcagcggta aacagtaata gtttgtcttt
     6541 ttctcttgga gtcagttcca tagcgttctc ttaagtcagc cagatccggg gaagtaccgg
     6601 tgatttgtca gtcagttgcg gccgcataaa ttgccagata tcacgcatta cctactggca
     6661 acgcagatta tcatcactta aaaagcgaac tgtcagtaga ccatcggtga gcgtggctcc
     6721 ggcgtagttt tccagtggtg tgagtttttc gcgtatgtct tccagttgcg tttcattccc
     6781 caggtagaac agcatcgttc ccacccatgg tttccgggca acgcaggtaa gttctccatt
     6841 ggccacgtgc agacgctcaa tcaacagcgg agaaccgtcg acccatatct ccagacggtt
     6901 agcgagtgtt ccgtggctga aggtttctcc gatgaccgga cgtccaaggc acagaagatc
     6961 ccaggccagc agtgtactgg aggacgacag atgaaacacg gtatgcaacg tggcgtgagc
     7021 gccgggaaaa aaaatcgaat cctgtggcaa ccactccagc gttgcgttat tgcacagcgt
     7081 caggttttgc tgaaggtgcg cctgcgcgcc gctgctgcgg taaaatttac tggcaccagg
     7141 catggtgatc agcgtatggc aatcagagtc aattattgcg ttgatgatga gttcgtcacc
     7201 gccgacgata ccaccgggtg gatgaagcag acagaggtgg caggtttctt cttcgggata
     7261 aaaaggacgc tgaacagtga gcagaccgac atgatgacgg gtggtgagcg tggttttccc
     7321 gtgcagacgc tgaaaacgga gatccagcat ggcctgccag ccttttttat tttgtggtaa
     7381 cgcggtactg agcaatctta cgcccctgat aaataaagat aaaacgacag taaagagccg
     7441 gatttccttg ttatataaaa gatgatgcct ttccagtgag ttttcgtctg aataatacga
     7501 atgtttgttt tcgtcatgaa gcgttccggc tcccggggct atacatcgcc agaaaagcaa
     7561 tgcatattgt cgggattatc cagagaatca ggcagaaaga tccgacaatg ggctgtgatt
     7621 atgccatctg gctcagaatg atatcaaggt gttatgggga taacttgctg atacaatagc
     7681 agtatgacag attcattaat gcggaaactg ctcactgaaa cagagtgttc atgactgagc
     7741 actggtcggg tcatgcagat gataatttcc ccttttcttg gggaagcgac agtattttca
     7801 tgcatgcctg tcttcagtca tacggggaga gcatctgtga tacagtccat acatttgttg
     7861 cggacgtcgc tcctgagtca ctgactggtc tgcaaacagt ttatctgtac acggcagggc
     7921 ttctgtgtga tatcgttttt catatcgagg gggggcctcc agagatgaat ggaataataa
     7981 tatttcctga tgatataata ccttagagaa
//
LOCUS       DAZKIF010000171         7968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZKIF010000171 DAZKIF010000000
VERSION     DAZKIF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.172"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(46..288)
                     /locus_tag="ACHOTW_004207"
     CDS             complement(46..288)
                     /locus_tag="ACHOTW_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9846208.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            420..1703
                     /gene="ydfJ"
                     /locus_tag="ACHOTW_004208"
                     /pseudo
     CDS             420..1703
                     /gene="ydfJ"
                     /locus_tag="ACHOTW_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1792..3252
                     /gene="ydfI"
                     /locus_tag="ACHOTW_004209"
     CDS             1792..3252
                     /gene="ydfI"
                     /locus_tag="ACHOTW_004209"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9846209.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3288..3491)
                     /gene="ydfZ"
                     /locus_tag="ACHOTW_004210"
     CDS             complement(3288..3491)
                     /gene="ydfZ"
                     /locus_tag="ACHOTW_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP9846210.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3668..4354)
                     /gene="rspR"
                     /locus_tag="ACHOTW_004211"
                     /gene_synonym="ydfH"
     CDS             complement(3668..4354)
                     /gene="rspR"
                     /locus_tag="ACHOTW_004211"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP9846211.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4443..5189)
                     /gene="ydfG"
                     /locus_tag="ACHOTW_004212"
     CDS             complement(4443..5189)
                     /gene="ydfG"
                     /locus_tag="ACHOTW_004212"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP9846212.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5326..7371
                     /gene="dcp"
                     /locus_tag="ACHOTW_004213"
     CDS             5326..7371
                     /gene="dcp"
                     /locus_tag="ACHOTW_004213"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP9846213.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7416..7934)
                     /gene="ydeJ"
                     /locus_tag="ACHOTW_004214"
     CDS             complement(7416..7934)
                     /gene="ydeJ"
                     /locus_tag="ACHOTW_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFP9846214.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1995 a         1862 c         2013 g         2098 t
ORIGIN      
        1 gcgagtattg aatgatttcc agccgttact gattttactc gttaattagc gaacaaacca
       61 ctcgtcagca gactcccagg tatctttcag cgtctcctga acaaaagttt ttgcagaatc
      121 tttatcggcg gtgcgagtaa ctgaaaggcc atcgttgctg gtggatttga tgatcacttc
      181 tacatcgtca taacgtttac tgacacgtcg cagcatttct tgttgcaggg caggaacaga
      241 accttttggc attttgttga ttttggctgc ggggtatctg tggggcatat atgggacata
      301 aaaaggcctc aaataaacag caaaatcgag attagggatt tttagaaaaa atacaaccat
      361 ctgaaaaaac ttagaaaaca cccaaaaacc cacagaatag taaaaaacat ctatgaatta
      421 tggatttcca gttatattcg ctcggcgcag cgttagtgtt tcatgaaata ttttttcctg
      481 aatcatcaac ggcaatggcg ttaattctgg caatgggaac ctacggtgca ggttatgtgg
      541 cgcgtattgt cggagcattt attttcggca aaatgggcga cagaataggg cgtaaaaaag
      601 tgctctttat taccatcacc atgatgggga tctgtaccac cttaattgga gtgttaccga
      661 cctatgcaca gattggtgtt tttgccccca tcttgctggt gacgctgcgt attattcagg
      721 gattgggtgc aggtgcggaa atttccggtg ccggtacgat gctggcggaa tatgcgccaa
      781 aaggtaagcg cggaattatc tcctcatttg tggctatggg aactaactgc ggaaccttga
      841 gcgcaacggc aatctgggcc tttatgttct tcattctcag taaagaggaa ctgctggcgt
      901 ggggatggcg tataccgttc ctggcgagcg ttgtcgtgat ggtctttgct atctggttgc
      961 gtatgaatct gaaagaaagc ccggtctttg agaaggttaa cgacagcaac caaccgacag
     1021 caaaacctgc acctgctggt agcatattcc agagcaaatc cttctggctg gcaacagggc
     1081 tgcgttttgg tcaggcaggt aactccgggt taattcagac tttccttgca ggctatttag
     1141 tacagacgtt attgtttaac aaagcaattc caacagatgc attgatgatc agttcgattc
     1201 ccggctttat gaccattccg ttccttggtt ggttatccga taaaattggt cgccggatcc
     1261 cgtatattat tatgaatacc tccgcgattg tgctggcatg gccaatgctt tctatcatcg
     1321 tagataaaag ctatgccccg agcaccatta tggttgcact gattgtgatt cataactgtg
     1381 cggtgctggg attatttgct ctggaaaaca ttaccatggc agaaatgttc ggctgtaaaa
     1441 accgctttac ccggatggct atttctaaag aaattggtgg tcttatcgct tccggttttg
     1501 gtcctatcct ggcgggtatt ttctgcacca tgacggaatc ctggtatccg atcgccatta
     1561 tgatcatggc atattcagtg attggtttaa tctctgcgct gaaaatgcca gaagtgaaag
     1621 accgtgattt aagtgcgctg gaagacgctg cggaagatca accgcgtgtt gtaagagctg
     1681 cgcaaccttc cagaagtctg taaatcctca atcccttctc ttaccggaga ggggattttt
     1741 attcatataa caaaacatgt agcttgccat atttaaattt aaggaattgt tatgggaaat
     1801 aatttgttat cagcaaaagc gacactccct gtttatgatc gtaataacct ggccccaaga
     1861 attgttcatt taggctttgg tgcatttcac cgtgcgcatc agggcgtgta tgccgatatt
     1921 cttgctacag aacatttcag tgactgggga tattatgagg tcaacttaat cggcggcgaa
     1981 cagcaaattg ccgatttaca tcagcaagat aatctttata ccgttgcgga aatgtcggcc
     2041 gatgcgtgga cggctcgcgt cgttggcgtc gttaaaaaag ccttgcacgt acagatagat
     2101 ggcttagaaa ccgtgttggc tgcgatgtgt gaaccgcaaa tcgcgattgt ctctctgaca
     2161 atcaccgaaa aagggtattt ccactctccg gcgaccggac agttaatgct cgatcacccg
     2221 atggtcgctg ccgacgtgca aaatccccac cagccgaaaa cagcaacagg ggtgattgtt
     2281 gaggcgctgg ctcgccgtaa agcggcagga cttcccgcat ttaccgtcat gtcatgtgac
     2341 aacatgccag aaaacggtca tgttatgcgt gacgttgtca cttcctacgc acaagccgtt
     2401 gatgtaaaac tagcacaatg gatcgaagat aacgtgactt tcccatcaac aatggtggac
     2461 cgtattgtgc ccgcagtgac agaggatacg ctggcgaaaa tcgaacagct taccggtgtg
     2521 cgcgatcctg ctggcgttgc ctgtgaacct ttccgccagt gggtaatcga agataacttt
     2581 gttgccggac gtccggaatg ggaaaaagcg ggagccgaac tggttagtga tgtgctgcct
     2641 tatgaagaga tgaagttgcg catgctcaac ggcagtcatt cattcctggc gtatctgggt
     2701 tatcttgccg gatatcagca cattaatgac tgtatggaag atgaacatta tcgtcatgcg
     2761 gcgtatgcct tgatgttgca ggaacaagcg ccgacgctga aagtgcaggg cgttgatttg
     2821 caagattacg ctaaccgatt aattgcacgc tatagcaacc cggcgttacg tcatcgaacc
     2881 tggcagattg cgatggatgg cagccagaaa ttgccacagc ggatgttgga ttctgttcgc
     2941 tggcatctgg cgcatgacag caagttcgat ctgctggcgc tgggcgtcgc gggttggatg
     3001 cgttatgtcg gtggtgttga tgaacaggga aatccgatag aaatcagtga cccactgtta
     3061 cctgttattc agaaggctgt acaaagtagt gccgaaggga aagcgcgcgt ccagtcattg
     3121 ctggcgatta aggcgatttt tggtggtgat ttgccagaca atagcttgtt tactgcaaaa
     3181 gtgacggaag cgtacttgtc tttattagcg catggtgcga aagcgaccgt ggcgaaatat
     3241 tccgtgaagt aaaaaaagca atgccgccat cggcggcatt cacacgctta gttcatgccg
     3301 agacgaatca ggtccagtgg tgccagtttc tcttcacaac cttcaacaac tacggttttt
     3361 ccgcggcgga tttgctcagc ggtcagacct tcagtatcaa tcgacaggat cttgccagtg
     3421 tgaccggtgc cgctaaccat aacacggctg ccagtggtga ttgcgttacg gttacgatcg
     3481 taggtcgtca taggtgtttt ctcctttctg atttacagtc gaagcccacg taacgggcgc
     3541 aatataaata ctcctgtcga gtgaaatatt ttttgttttt gatcaagctc acatcttttt
     3601 tgatgcatgc atgagccata aggcggggga aaagactctc accccggatg ggatgagagg
     3661 aaatgaatta ctcttcgcta aaccagtcgc tattttcctg gcggatctgg cgcacggatt
     3721 cgctgatttc ctgcaaatgt tgcgtcattg cacgttctac cgcatcgcca tcacgttttt
     3781 gcagggcaga gaatatatca agatgctggc gtaacagcat ttctggtgga gaaacatggt
     3841 cgaaactcat atagcgcacg cgatcaacgg tcgctttcag gttctcaatg gtatcccacg
     3901 ccagttgaca gtcggcaatc tgcgtcagga gttgatggaa gttgtcatca agttcaaaaa
     3961 aatcatccag ttgcttgcgc tcaatggcaa tgcgttgctg gtgaagattt tgttccagtt
     4021 ggtagcactg gctttcggta atcatactcg ccgcccgacg cgccaccgcg cactcaatgg
     4081 cctgacggat aaaactgccg ttgcgcacct gggccatgga aattttgttg acgtagctgc
     4141 cacgctgcgg acgaatttga atcaggccgt tttccgccag tttaataaag gcttcacgaa
     4201 ccggctggcg tgacacattg aaacgaacag aaacttcttt ttccgacaac ggtgtgcctg
     4261 gtgcaatcag gcaatggaca atgtcgcgac gaagaatacg ataaatctgc tgattgacag
     4321 gctgtgtggg attaagttgc gtttcgacgg tcattcgttc ttgcttgtga gtgagttaac
     4381 tgcatgagtc taccactttt ttgtgacaag caataacccg gcagttacac cgggtataaa
     4441 aattactgac ggtggacatt cagcccggca tagctttggg taaccggcat catttccagg
     4501 gtattgatat tgacgtgagc aggcagcgtt gacacccacc agacggcttc gctgacatct
     4561 tctggcgtta atgcaacggt attttgatag gttttttccg ctttaccgtc atcgccttta
     4621 aagcggacat tggaaaactc ggtgccaccc accagacccg gttcgatgtc ggtgacgcgc
     4681 accgccgtac catgcaggtc cgtacgcaga ttcaggctaa actgacgaac aaacgctttc
     4741 gtcgcaccgt aaacgttacc accggcatac ggccagctac ctgccgttga gccaatgtta
     4801 ataatatgac catgattacg ttcaaccata cccggtaaga cggcgcgcgt catatatacc
     4861 aggcctttgt tgttggtatc aatcatcgtt tcccagtctt caatgctggc tttatgcgca
     4921 ggctccatgc ccaacgccag accggcatta tttaccagga tatcaatatt gcaccactcg
     4981 gcaggaagcg atgccagcat ctcttcaata gcggcgcggt tgcgaacgtc cagttgggcg
     5041 atgtacagat tatctcccag ttcgtctttt aactcctgca accgttcctg gcgacggcca
     5101 gtggcgataa ctttatgccc ttgttgaata aaacgacgag taatgcattc accaaaacct
     5161 gccgttgctc cagttactaa aacgatcatc tcactgttcc tcaacgcttt tgtgtgtcaa
     5221 taacatagca cgtgatgctt aagagcgggt aatactggtt tgcaagattg cactccgtta
     5281 tcagtaagcc tacgctatgg aacacctcgc gttcaggaga agaaaatgac aacaattaat
     5341 cctttccttg tgcaaagcac actgccgtat ctggctcctc attttgatca aattgccaat
     5401 catcactatc gcccggcatt cgatgaggga atacagcaaa agcgggcaga aattgctgcc
     5461 atcgcgctta acccgcaaac acctgatttc aacaatacta ttctggcact ggaacaaagc
     5521 ggagaattac ttacccgcgt taccagcgtc ttttttgcga tgactgcggc gcataccaat
     5581 gatgaattac agcgtcttga cgagcagttt tccgctgaac tggcggaact ggctaatgat
     5641 atctatctga acggtgaatt attcgcgcgg gtagatgctg tctggcaacg ccgtgaatct
     5701 ctggggcttg atagtgaatc catccgcctg gtggaggtga ttcatcaacg ttttgtccta
     5761 gccggagcca aacttgcaca agctgataaa gcaaaattaa aagtactgaa tacagaagct
     5821 gcgacactga ccagtcagtt taaccagcgg ttactggcag caaataaatc cggcggtctg
     5881 gttgtgaacg atatcgcgca gctggcagga atgagtgagc aagagattgc gctggcggca
     5941 gaggcggctc gcgagaaagg tctggataac aaatggctga ttccgctgct gaataccacc
     6001 caacaaccgg cgcttgccga aatgcgcgat cgtgcgacgc gtgaaaaact gtttattgcg
     6061 ggctggacgc gagcggaaaa aaatgatgcc aatgataccc gcgctatcat tcaacgtctg
     6121 gtggagatcc gtgcacaaca ggctacatta ctcggttttc ctcattatgc cgcatggaaa
     6181 atcgccgatc agatggcaaa aacacctgaa gcagcactca actttatgcg ggaaattgtt
     6241 ccagcggcgc gtcaacgtgc gagcgatgaa ttagcctcca tacaggcggt tattgataag
     6301 cagcagggcg ggtttagcgc gcagccgtgg gactgggcat tttatgccga acaggtccgg
     6361 cgagagaaat ttgatcttga tgaggcgcag ctcaagccat attttgaatt aaacacggtg
     6421 ttgaatgaag gtgtattctg gaccgcgaat cagctcttcg gtattaagtt tgtcgaacgt
     6481 tttgatattc ctgtctacca tcctgacgtt cgggtgtggg aaatttttga tcataatggc
     6541 gtggggctgg cgttatttta cggtgatttc ttcgcccgtg attcaaaaag cggcggtgca
     6601 tggatgggca attttgttga gcaatcaacg cttaatgaaa cgcatccggt aatttataac
     6661 gtctgcaatt atcagaaacc cgctgccggt gagcctgcgt tgttactctg ggatgatgtc
     6721 ataaccttat tccatgaatt tggtcatacg ctgcacggcc tttttgcccg ccagcgttat
     6781 gccacgctct ccggcaccaa cacgccgcgt gattttgtcg aatttccgtc gcaaatcaac
     6841 gaacactggg caacgcatcc gcaggtattc gctcgctacg cccggcatta tcagagcggg
     6901 gcagcaatgc ctgacgaact gcaacagaaa atgcgtaatg ccagcctgtt caacaaaggg
     6961 tatgagatga gcgaactgct tagcgccgca cttctcgata tgcgctggca ttgcctggaa
     7021 gaaaacgaag caatgcagga tgtcgatgat tttgaattgc gggcgctggt ggcagaaaat
     7081 atggatcttc ctgctatacc gccacgctat cgcagcagtt attttgccca tatttttggt
     7141 ggcggatatg ccgcaggtta ttacgcttat ctgtggacgc aaatgttggc cgatgatggt
     7201 taccagtggt ttgttgagca gggcggatta acgcgtgaaa atgggctgcg ttttcgcgag
     7261 gcgatccttt ccagaggtaa cagcgaagat ctggaacgcc tgtatcgaca atggcgcggt
     7321 aaggctcctc agattatgcc gatgctgcaa catcgtggct tgaacatata aacaccacca
     7381 atggctgtcc tggagatatc gccaggccag cctggttata gcagtaactg cagcagctgg
     7441 gcgagggcaa acctcaccgc taaagctaat accgtttcgc agtcgccagc aaaatgcata
     7501 accgcagtgt agttctggcc tttaatatgc cacgcgaacc agacggtacc cgctggcgta
     7561 ccatcttcac cgccctccgg tccgccgtag ccggtaatgg caatactgac atcagcatcc
     7621 gcacgctcta tggcaccggt tgccatttct gctgccactt tctcactcac cgcagaatat
     7681 cgttcaagag attgctggct tacgctgagg attttcatct ttgcctgatc ggtgaaagta
     7741 acaaagcctg caccgtaaaa tttgggtgta tcttcagctg cacacagggc gctagccaac
     7801 ttaccgccgg tgcatgattc ggcggtagtt aagcgtaatt gatccgcgat aagtctttga
     7861 ctcaacgcgg atgtcaggtt ttcaatagtg tcggtatctg caagttgtac aattttattt
     7921 ctattaatgt tcactgtttt ttgtccacgc ttttatatgt aaaggtga
//
LOCUS       DAZKIF010000172         7841 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZKIF010000172 DAZKIF010000000
VERSION     DAZKIF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.173"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(50..1414)
                     /gene="tdcG"
                     /locus_tag="ACHOTW_004215"
     CDS             complement(50..1414)
                     /gene="tdcG"
                     /locus_tag="ACHOTW_004215"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9846215.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCEPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1486..1875)
                     /locus_tag="ACHOTW_004216"
     CDS             complement(1486..1875)
                     /locus_tag="ACHOTW_004216"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP9846216.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1889..4183)
                     /gene="tdcE"
                     /locus_tag="ACHOTW_004217"
     CDS             complement(1889..4183)
                     /gene="tdcE"
                     /locus_tag="ACHOTW_004217"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP9846217.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4217..5425)
                     /gene="tdcD"
                     /locus_tag="ACHOTW_004218"
     CDS             complement(4217..5425)
                     /gene="tdcD"
                     /locus_tag="ACHOTW_004218"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP9846218.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5451..6782)
                     /gene="tdcC"
                     /locus_tag="ACHOTW_004219"
     CDS             complement(5451..6782)
                     /gene="tdcC"
                     /locus_tag="ACHOTW_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP9846219.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6804..7793)
                     /gene="tdcB"
                     /locus_tag="ACHOTW_004220"
     CDS             complement(6804..7793)
                     /gene="tdcB"
                     /locus_tag="ACHOTW_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP9846220.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         1988 a         2083 c         1891 g         1879 t
ORIGIN      
        1 aaaaaaaggt gcacatttgt gcacccaagg atgaaagctg acagcaatgt cagccgcaga
       61 ccactttaat ggccagtccg ccgcgtgatg tttcgcggta tttatcgttc atatctttgc
      121 cggtttcata catcgtctcg atcactttat cgagtgaaac acgcggtgcc gaggtgcggc
      181 gcatcgccat ccgcgcggcg tttactgctt tcacggcatt aatggcatta cgttcaatgc
      241 acgggatttg tacctgtccg gcaaccggct cgcaggtcag tccaaggtta tgctccatcg
      301 cgatttccgc cgcattgcat acctgcgccg gactgccgcc cagtagttca gttaaccctg
      361 ccgccgccat tgaacaggcc acgccaatct ccccctgaca gccgacctcc gcgccagaga
      421 tggaggcgtt cattttatac agcgcgccaa tagccccggc tgcaagaaaa tagcgggcaa
      481 ttgaccgctc gtttaccgga cgacggaact tatcgtaata agccagcact gccggaataa
      541 tgccgcacgc accgttagtc ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac
      601 taaccgccag cgcgtacatg ttgatccagt cgatgacatt catcggatcg ttagagatgt
      661 tatcgctgga aaccagctga cgacgcagcg ctacggcacg gcgcggcaca ttgagcggac
      721 caggcagcac gccttcagtg ttcatcccgc gttcaatacc gtcatgcatc acttgccaga
      781 tacgggcaaa accggcgtca atttccgctt tactgcgcag cgctagctcg ttgtgcatca
      841 tcaggccaga aatagacagg ccgttgtaat cacacatttt cagcagttca cctgctgagt
      901 ggaaatcgta aggtacgggt gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga
      961 caataaaccc gccgccgaca gagtaatagg ttttacttaa tagctcttcc tgtgccttcc
     1021 aggcagtgat ccgcattccg ttctcatggc gaggcaacat ttcgggatgg aagataatgt
     1081 tctttgctac aggaaaatca acaatatgcg caccagatgc cactggcagc cgcccgctgc
     1141 gcgttactaa ctctataaat gcagggatct catcaatgac aacatcctgc ggactgtttc
     1201 ctgccagtcc catgatgatg gcgacatccg tggcatggcc tttgcccgtc agtgacaacg
     1261 acccgtacag atcgaccaca atatggctcg tcgcggttaa taagccgcta ctttccagcc
     1321 gatcaataaa actttttccg gcattcattg gccccacggt atgcgaactg gagggaccaa
     1381 tcccaatttt gaaaatatcg aatgcactaa tcatatccac accctcggat tgccgttcag
     1441 tgaagtggag cggaacgacc ttacgaccgt cccgctcacg aggctttacg cactacgtac
     1501 tgcgatagct tcaatttcca gcttcacatc tttcggcaaa cgcgcgacct gtacacagct
     1561 ccgggtcggg taggtcgcct gatgctcatc gaagaattgc ttatagactt cgttgatggt
     1621 ggcaaaatca ttcagatcgg tgatgaacac ggtcatcttg atgatatcgc ccacgctcag
     1681 cccggcagca accacgatcg ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac
     1741 atcagccggg atctcaccgg tctgtgggca aaccggaatt tgaccggagg tgaagaccat
     1801 gctgcctaaa tcaacgcctt gaacataagg gccgattgcg cctggggcac gttgcgtttc
     1861 gataatcttt ttcatacatc ctccggcgtc agagcgcctg ggtaaaggta cgtgaaataa
     1921 catcctgctg ctgttcacgg gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga
     1981 ttgtcaggtt aggatatttt tccgggtgct cgatggcatc cagcagcatt tcccgattca
     2041 ttacgttgac gttgaggtgt tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat
     2101 ccagcaggcc gacaaggttg gttttacgta ctggatcttc tttgcccagc gccgcaggga
     2161 cgattgagaa ggtgtacgag atcccatctt tggcgtaggt gaacggcagt ttcgccaccg
     2221 acgtcaatga ggccacggca cctttgcggt cacgaccatg catcgggtta gcgcccggcg
     2281 cgaacggtgt tccggcgcga cgaccgtccg gcgtgttacc ggttttctgg ccgtacacca
     2341 cgttagaagt gatagtcaga atcgactggg tagggacggc gttgcgatag gttggcagcg
     2401 ctttaatttt cttcataaaa cgttcaacca ggtcgcaggc aatgctgtct acgcgctcgt
     2461 cgttgttgcc gtactgcgga tattcaccgt cgatttcaaa gtccaccgcc aggccgtttt
     2521 tgtcacggat tggtttcacg cgggcatatt tgatcgcaga cagggagtcc gttgccaccg
     2581 acaggcccgc gatgccgcac gccatggtgc gatagacatc acgatcgtgc agcgccatca
     2641 gcgaagcttc gtagctgtac ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt
     2701 actgcaccgc cagccagtcc atgaagtgat cgaggctgtc catcactttg tcgtaatcca
     2761 gcacatcgtc catcaacggt gccgttttcg gcccgacctg aatcttcagc ttctcgtcca
     2821 ccccgccgtt aattgcgtac agcagtgttt tcgccaggtt agcgcgtgca ccaaagaact
     2881 gcatttgctt accaatcacc attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt
     2941 tgaagtcagt acgcatcaga tcgtcgtttt catactgcaa ggaagaggtg acgatcgaca
     3001 cctgcgcggc atattttttg aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt
     3061 taggttccgg tgcaggcccc atagtgtgca gggtgtgcaa atagcggaag gagtttttgg
     3121 tcaccagcgt acgaccgtcc agccccatcc cgccgatcac ttccgtcgcc cagattgggt
     3181 cgccggagaa cagcgaatca aattccggcg tacgcaagaa gcgcaccata cggatcttca
     3241 tgatgaagtg atcgatcagt tcctgtgcct gctgctcatt gagtacgcca gctttaaagt
     3301 cgcgctcaat gtagatatcc aggaacgatg ccgtgcggcc cagcgacatc gcgccgccgt
     3361 tctgtgattt cactgccgcc agataagcga agtagagcca ctgcaccgct tcctgcgcat
     3421 tctgcgccgg gcgagagata tcaaagccat atttcgccgc catttcctga atctgcaaca
     3481 gcgcatgacg atgctctgcc agctcctcac gcagacggat ggtggcttcc agatcctcgc
     3541 ctttttccag acgagactgg agatcggcaa attgcagttc gcgttcacgc accagataac
     3601 tgatgccata cagcgctacg cggcgatagt caccgataat gcgcccacgg ccatagccat
     3661 ctggtaaacc ggtcagcacg ccagatttac ggcagcgcag catatccggt gagtaaacat
     3721 caaatacgcc ctggttatgg gttttacgca gatcggtaaa cagatattca aattcactgt
     3781 ccatttcacg accataggcg tggaatgaac ttttaatcat attaatgcca ccgaacgggt
     3841 gtaacgcacg tttcaacggc gcatccgttt gcaggccaac aattttttcc agcggctggt
     3901 taatatatcc cgcatcatga gcggtaattg tggtggcaat attggtatcg aaatcaaccg
     3961 gcgcgtgggt tgcgttttca atacgaatgc cttccatcac cttttcccac aattccgtgg
     4021 tggctggcgt cgcttcagcg aggaaagatt catcgccttc atacggtgta tagttatgtt
     4081 gaataaaatc gcggacatta atttcgtttt tccagtccgt acctttaaag ccaagccatg
     4141 cgtcggcgta cagcttatcg ctggtatcaa tatctacctt catgaaaaat aatctctcta
     4201 caatacttca actaaattat gcaaattctg cgggtgcgtt aactttgcct aaatgaatgg
     4261 catccaaagc aatcattttt tcttcgttgg tcggaattac ggcgcaaatg acacgcgcat
     4321 tttcactgga aacaattcgc tcgccaaaag agttagatcg attattcatt tctgtatcaa
     4381 tctctacgcc taatacagcc aaatgttcca tgaccagacg acgaattaaa cttgaattct
     4441 ctcctattcc gccggtgaat ataattccat ccaggcgacg taatgaagct gcgtgtccgg
     4501 caatatgacg ggcaattcgg tgaacaaagg ttttaattgc cagctgcgcg cgttcgtgac
     4561 cttcatgcca ggctttttcc agaacacgta aatccgaaga aagaccggaa atacctaata
     4621 atcccgactc tttattcact acgcgttcca ggtcacccag gctctggttg gtttggctgg
     4681 cgacccagga catcgcacca aagtcgacat cgccactgcg ggtacccatc atcaagcctt
     4741 ccagcggcgt cattcccatt gaggtgtcaa cactctgacc gttgcgaact gcgcagattg
     4801 acgcgccatt gccaagatgc gccacaacca ggccggagtc atcttccgcc agattcagca
     4861 aagaatgtgc gcgcagggaa acatagcggt gcgacgtgcc gtggaaacca taacggcgta
     4921 cacctaactc ttcataatat ttccacggca ggccgtataa ataagcttcc ggagccatcg
     4981 tctggtggaa actggtatca aataccgcca cctgagttac gcccggaaat aattgctgcg
     5041 ccgattcaat accacttaaa ttggcgtaat tatgcagggg tgccagtgga gaaacgcgac
     5101 ggatattatc aatgacttca tcggtaataa tggcggactc ggtaaaaata ctgccgccgt
     5161 gagcgatgcg gtggccaatt aaggccacgc tgtcatttaa attccgtttt tccagttcaa
     5221 atgcaattgc cttcaatgca ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc
     5281 catttacgga taagaatgca ttttccgagt taataccgtc ggcaatacct gacattaata
     5341 cttcacagtc gctggcatcg agtacggaaa acttaatcga agacgaacca cagttaataa
     5401 ccaaaacaac cggaaattca ttcatctctt ttctcatcct gagttacgga ttaaaacagt
     5461 ttatatacga tgttcaggat ggtcagcaga ccaatcacgg taacaaacac gttatccaga
     5521 cgaccacggt atttcgccag agacggcgct ttacggatgg catacatcgg caacaggcac
     5581 agcagggatg cgataatcgg tgcgcccatg gcttcaatca ggtcgaggat gttcgggttg
     5641 gcgtaggcaa caacccaggt ggagcccatg atgaagatca tgctgatagt gttcagttta
     5701 cccagcgata ctttagtttt gtcgccttta taaccaaact tcaggaccag gccattcaga
     5761 ccttccagcg ttcccagata gtgaccgaag aaagatttga agatagccac gagtgcgatg
     5821 atggaagccg catattccag tgtaatcgcg aacgttgttt tggtaccggt catggacgca
     5881 aagtggttag ccagataaga aagcactgga atattctgcg ctttggcttc cgccatgttg
     5941 gccggagaca gcgtaaacag gcagctaaag gcaaagaaca tcaccactgc aaccatcagc
     6001 atgctggcac gagaaatgat ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct
     6061 ttctcatact cttcacgttt agaaaccacg aaggaagaga cgattggcga gaagttaaag
     6121 gagaaaacca tgatggaaat ccccagccag acagtgatca ggataccgtc atgaccggtt
     6181 aacgacagcg aaccgaggtc aacctggtcg ataactgcag agttccagta agggatcagc
     6241 gacaaagaaa tcagcaccag gctggcgata aacggccata ccaggtagct catcacttta
     6301 accatcagat ccttaccaaa ccagatgacg aaagccatca gcagcaacag gaacagcgcc
     6361 acaaagccgc gattcagcgg tgcaaagccg agctggtttt cccagaacgt cataaaggta
     6421 ttggtaatag taacgccata aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac
     6481 gtgataacca cgccgccagt tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg
     6541 gaagggttag agccagaaag acacagacgc gccagcgccc ggtggcaata aaatgcgatg
     6601 gggtatgcca ataccaacat cagaagaatc gggatcagtc cgccaaaacc tgcgcggata
     6661 gggaagaaca gcaccccggc gccgattgcc gtaccaaaca agcctaacgt ccatgtggta
     6721 tctgatttac gccaggacga ttgttttgtc tggctggata caatgctatc tgaagtactc
     6781 atatcctatc ctcaacgaat taattaagcg tcaacgaaac cggtgatttg agagacgcga
     6841 gaaagatcga tattgccgcc ggaaataata ctgacggttt ttctgttttg aatatattgg
     6901 tctaatttac cgcttaataa tgcagcacat gccagagcgc ctgcgccttc ggtgacgact
     6961 ttattgcgct gaattaaggc aatcatactg tttctgattt cgtcttcgct gaccagcacg
     7021 atgtcatcga ctaattcacg aacgatttcg taagttaaat tacccgggcg ggagacatca
     7081 caaccatccg ccagggtgcc ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa
     7141 gccgccatgc cgtgaacgtt ttcagactgt acgccaataa cacgaatggt cgggttaata
     7201 gatttaattg ccaccgcaat accagcaatt aaaccgccac caccaattgg cacaatcacg
     7261 ttatcgacat catagagatc ttccataatt tccaggccaa tcgttccctg accagcaatc
     7321 actttcggat catcgtaagg tgggataaaa atacggcctt ccatttcgac aatttcgctc
     7381 actttagcga tagtgtcgtt gaagttatca ccatgcagaa cgacttctgc ggagtagtcg
     7441 cacgttgccg ctactttgga tttcggcgcg ccttttggca tcaccacttt accgtcgata
     7501 cccagcatcg cgcaggagag cgaaacccct tgcgcatggt tgcccgcaga acaggccacc
     7561 acgcctttgc gtttttccgc atcagtcagt gaacttaatt tattaaatgc gccacgaatt
     7621 ttaaatgaac ccgtacgctg catattttca aacttcagga atatttcacc tttgcaacgt
     7681 tcactaaaat agttggagcg aggcatgcct gttttataaa ttcgcccagc cagtcgttgt
     7741 ttcgcttcaa taatgtcatc aatagcaacc ggcagatcgt atgtaatatg cattataaaa
     7801 cctcttcgcc tgaattaaat atgtaggtaa ccgtaaccga c
//
LOCUS       DAZKIF010000173         7816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZKIF010000173 DAZKIF010000000
VERSION     DAZKIF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.174"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..777
                     /locus_tag="ACHOTW_004221"
     CDS             <1..777
                     /locus_tag="ACHOTW_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9846221.1"
                     /translation="FSIEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANLLL
                     VDIKEPDNRYLKHLTHEGVEVEFMTIDITKPDASCTIINRCLERFGQLDILVNNAGVC
                     NINRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKIINMCSVLSFHGGRWS
                     PGYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKGRI
                     PAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
     gene            complement(923..1612)
                     /locus_tag="ACHOTW_004222"
     CDS             complement(923..1612)
                     /locus_tag="ACHOTW_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9846222.1"
                     /translation="MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(1609..2943)
                     /locus_tag="ACHOTW_004223"
                     /pseudo
     CDS             complement(1609..2943)
                     /locus_tag="ACHOTW_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(2959..5481)
                     /locus_tag="ACHOTW_004224"
     CDS             complement(2959..5481)
                     /locus_tag="ACHOTW_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9846223.1"
                     /translation="MNKSVVSISAAMLVLLCQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVAATNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDLTWQHGMTNTLTFNSG
                     SRIADGYQALMLGGVYGSSLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGITFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSERQVAP
                     VAGAAVKVIFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            complement(5534..6235)
                     /locus_tag="ACHOTW_004225"
     CDS             complement(5534..6235)
                     /locus_tag="ACHOTW_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9846224.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            complement(6321..6881)
                     /locus_tag="ACHOTW_004226"
     CDS             complement(6321..6881)
                     /locus_tag="ACHOTW_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9846225.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLMPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            complement(6924..7547)
                     /locus_tag="ACHOTW_004227"
     CDS             complement(6924..7547)
                     /locus_tag="ACHOTW_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846226.1"
                     /translation="MNKNLLTRVIIISDDLYYLQGLSAKLGHHYRFFIECFFCSQNAC
                     NNDLSTLRFDNRFTKNVLLVAVDDVSILAKLPDLARFLMIVSLRKMKNRNIFFSLSEQ
                     TFISRYIKLNKLITLIKSTDKDQQVRQVTLTRYEWDIYYLYFKIDNKRILHTLLKKDV
                     KYISHYKISVFNKFEIACDSDGFYIFKALIQLRRFTGFKNVRLYQLH"
BASE COUNT         2031 a         1785 c         1849 g         2151 t
ORIGIN      
        1 ttttctattg aagatttttc attggatttt ttttcattac ataacaaggt ggccatcgta
       61 actggggccg cgggtgagct ggggagaggc ttatgctcgg ctctggccaa agcaggggct
      121 aatctattgt tggttgacat caaagagcct gataatagat atttgaagca tttaactcat
      181 gaaggcgttg aagttgagtt tatgacaata gacataacaa aacctgatgc atcatgcacc
      241 attattaata ggtgtctgga gcgtttcggt cagttagata ttctggtcaa taacgcgggc
      301 gtgtgtaaca ttaatcgtcc tatcgatttc aatcgaaacg actgggatcc gatgattaat
      361 ctcaacctga atgctgcatt cgatatgagc caggccgcat tgaatatatt tgtcccgcaa
      421 agaaaaggga aaattattaa tatgtgttcc gtattgtcat ttcatggggg acgttggtcg
      481 ccagggtatg cggcgactaa acatgcttta gcggggctga ccaaagcgta tgccgatgat
      541 tttgcggaat ataatattca gattaatggt atcgccccag gatattatgt gagtgaaatg
      601 accgctatta tttataataa ccctaaaata aaagagttaa taaaagggcg tataccggct
      661 caacgctggg gaagggcaca ggatcttatg ggcgcaatgg tgttcctcgc cagtgccgct
      721 tcagattatg tcaatggaca actattagtc attgatggtg gatattcaat tcgttaacga
      781 tttttggctg cgttgaggtg ttttgactgg aaaatggagt ggagagattt tgtcatttct
      841 ccacttccgg tacttcaggt gggtatagtg gttattcgtt agcgttgaac catcgcgatg
      901 agtttacata ccactcagag cattattgcg ctaattgttg ttttatctgt gcaccattat
      961 catcgagata tgtcgccgca accacaccac taacgttatt acttgctaac cagtaccgtc
     1021 gttgactgaa gggttttatc atcatatcgc tttcttgctg cacctttgta gccccaatgc
     1081 tgatggaggt gaaagagata ttcagtggtg ttgggttatt aagctcaata tagctactgc
     1141 tgccgtcgtt acccagatgc caacttattt tacgcatagc ttcttggggc gtcatagtta
     1201 cagattttgg cctgtaaaag attttaagtt gcgtattcat tgccagcgtt aaatgtgtgg
     1261 gatcttgatg gggagtttct ggcggcattt cataaagatt taaccagaat gcagattcgc
     1321 gatcctgggg aagtgggtca tgattataga taatccgcaa cccctgggtt cgtctcgcag
     1381 ccaggcggaa aaccggtggc agcaccacaa aggggggaga agcagcttct ggggaacctt
     1441 caccattatc tacccaactt tgcaccagta ccggatagtc attggtattg accagcatca
     1501 gtgtcttttc ccgatcgcca tcgcgataaa taatccgtga agaagccggc atgatgccgg
     1561 cttccactga ctgtacgaac aagatggtta agagcagcaa aacggatttc attgcatttt
     1621 caccaatata taagccgttg cccgcacttt tcccgcactg actgtctgat ttggcagttt
     1681 cttcaaccgg gcgataaaag agtaactata gctggagtat ccggggtgag tcgcaccttc
     1741 taatgttgcc ccttcaaata caggatacca ccccgctgca tttcctccgg gggttaactt
     1801 cgcgatgccc ggttgaccaa ccagcgtcat cgccgtatcg gggtgagcac tgttagaaat
     1861 gtagatccca acgccctttg ccatctctgc tgcgtcataa ttatcagaga ccagggcgct
     1921 gacgccgccg ttgctattga taatccccag tttttgcgcc gctgtatatg caccttcaga
     1981 cacctggatt cctaatgctg tctgagtatc gctaatccct gattgaaccg agttactaca
     2041 ctcgacgcgg acattaaaat taccggtact ttccatgccc gcattcagtt gtgcttctgc
     2101 aattgtcggc agcaatacta acggcgtggc gctgcgggca acgcaggtgg cattgttgta
     2161 gagtcgattg gccgaacgca ttccgtaacc aaaaccatta tcagcacccc agaaatccca
     2221 atgaacagaa ctatcttcgc cgggctcatc atgaccaaat aaaatgccgc tggtaccgga
     2281 aagctgaata taggcattgg gctgaacaca gttatagatt gtaccggaag gttttgcgaa
     2341 tccgactcca ctactatctg tattattatt accgcaccaa cttgttgttg ctgatgtatc
     2401 aggtagcgta ctgatgcgat aaagctcagc atgaagagga gggatatcct gtaagcgaat
     2461 ctggatttta gttccctgag tggcataact ggtgatgggc actttcttcc agtaacgacc
     2521 aagcgtcacg cccgccatgg tctgcttgag accgacaaaa gcgaactagg tggcatagac
     2581 atcactcaga ccatcaggcc cgcctgcatc gtaaaagccc cccacgcggt cgtcgccatt
     2641 ggtagcgacc agaaaataga tgtttggcaa atctgttgca tcacattccc acaatacgga
     2701 gcttcctgtc gcgccacctg atgtgtagtt agtgggaggt acgaccgttg tcgcgaggag
     2761 tgaacctgct ggcgcaaaat aggtgtcggt catattaatt tttccgaact caataagcgc
     2821 tgtccgacca tcttcagttt gacctatctg tgtgtttgta cagttagccc aagcggatga
     2881 ttgtattagt gccaccaata tgatggagct aatcagaatg tgtaaaaaag tcgatttaga
     2941 catcgggttt tctctgtatc aagactgaca ggtttcattc aggcggatga tagggctatt
     3001 gctgtccttc ccgctgatat caaagggcaa ttggcagcta tcgttagcac cttcacccca
     3061 gcgaactgac aagtggcctt ttgtctgttc tgtgcggagg taaatttgcc ccccctgacc
     3121 ggctataccg acgactgtat tattctcatc cagcacatcg gctcccattg gcagttccga
     3181 accatctgcc atgcgggatt taatcagcaa cgctttacca ggacgggtac ggaaaattac
     3241 tttcaccgcc gcacccgcaa ccggtgctac ctgtctttca ctgtcgacca actcggcatc
     3301 gccatccatt ccttgtggat cgagagatat acggttgtag cgatagggcg ttactgccgg
     3361 aacaagcgca tagccactgt cattaatttc cagctgactg gagttataga cttttgcacc
     3421 ttctgcgcct ttagcttcga ccagggcgaa cgtttcaccc agataaggtc cgaaagtaat
     3481 gccgccacca tgcacagcca tcgcgccttg tacgttacct gaagcctgcc agtaatcctg
     3541 gcccttcgat gcgttcaatc cgacggtagt ttgtgaaaaa cgtttttgca tgtttccgct
     3601 aagcgtcgtc tgcttatatt gttgatcgcg catgacgttc aggctgtagt tggtggtctg
     3661 tgcttcatca agcattccgg ttagcgagct ttgtaattgc gagctaccgt cagttgaatg
     3721 ggtccagctg ttgctaagac ttggtacacg aggtccattg ccgccaagtg ggaatgagaa
     3781 tgaaagggat gttaccgtct gcatatcatc agaattgtct ttatagccgc ccattctttg
     3841 gcgtccgacg gaaaggttca tactgatgcc atggctaaag ctattgctgt aacctaactg
     3901 aagctgtgta tcacggctct tgccgccacg gtagttctgt gttgaacctg acacaaacag
     3961 gttgccgtaa ttcgcaaggc tctgacttaa cgtaagatcg aagcgcgact gttgacgcca
     4021 ctggctggag tcccatgact gtttattgct ggcagcatga cgctctccca gcacatcagc
     4081 cagatcacga tagccgctgg tagagtatcg ataacctgcc agggagacgg tggttgaagt
     4141 aggctggaaa gttttactcc aggttaattg cgacatccaa ccactctgcg cttcgctttc
     4201 gggaacacgc gcatgggacc aagtgaggtt tgccccaaat gcccccagcg aactgccata
     4261 gactccgccc agcatcagcg cctggtagcc atcagcgata cgcgaaccac tgttaaatgt
     4321 cagcgtatta gtcatcccgt gctgccaggt aaggtcacca aacatcgagt catcaccact
     4381 atcctgcgtt ttacctactt ccacgttata acgggaagtt cctggacgca tcgattctgg
     4441 tacggctgaa aaggggacac tgaaacgact gactgcgccg ttagcttccg taacggtgac
     4501 atccagatcg ccgctgtagc tggtggggta taggtcgttt atctcgaaag ggccaggagc
     4561 cacggtggtc tgatatattt catgaccgtt ttgcattacc gagactctgg cgtttgttgc
     4621 ggccacgccg cgaatagtcg gcgcatagcc gcgcatggag tccggcagca tacgttcatc
     4681 ggtcgcgaga ctaacgccgt gataactgag tccagagaaa aatcttccgc tggtgataag
     4741 ctgccccatc attaactggc tatttatggc gggcagcggg cgttgcaaat agctacgaat
     4801 attgttccac tgattccctt tgtcattatc ccaggtcatg ttggataact gacgatattg
     4861 ccaggcacca aggttgatgc caccattaaa tgatgcccat agcgaacgct ggctatgagc
     4921 attctgccct gaataggcaa cgttgtaata gttggcaata taattcatga acgccagcga
     4981 tgctccggta tccagctcgc tggggggaac atagccgcga gggcgactaa tcaattgact
     5041 ctgtggcacc gataagtcca ggcgcaattg tgacaactgt gtctggctgg tgatgccggg
     5101 agcgagttcc ttagcaaacg tacagacagg atctttttcc cccttatctt cccctgtttt
     5161 gaggcctatc gcgtccgcga cttcaggcgt caggcagggc agaacgcgac catcagcggc
     5221 ttctttaaac gtgacattcc aactgcctga caacttattg ttggtgtaga tatccatttt
     5281 ataattgccc ggtgcaacag actcacgtgt tgtcagtttt gctaatgacg actgactaaa
     5341 tctgctgccg cggaagagtt gcgggtcaaa ggtgtagttg tcttcatctg acggtgttgc
     5401 gggtgagatt tcgctcccca tgaccggttg gcaaagtaaa accaacattg ccgcagaaat
     5461 tgacacaacg gatttattca tctcgacgta ctcgtgcgtg aaaaaggata ccgctcaaaa
     5521 aaggagaaag aggttataag gcatgctcag acgtaacatt tacgccatag tcgttgacgg
     5581 tcactaaatg gatctgcgca ccgggagcga cactgatgcc agagggtagt gtccattcag
     5641 ttgtgctttg aggagcaatc attaccgaat tcgcgagggg gactttttta ccattattaa
     5701 gcagttcagc ggcgcgaatt gtgacgtaat agcccgtggg attcgtcact tcaatgcggt
     5761 tctgttttac ctggtaagtc aggtttttgg gtgcgtcact ggatttaccg acaatcgagc
     5821 tgggacgata gaaaattttg actcgattag tcagggccag gatgagctgg ttctgacttt
     5881 tatcattctt attcagataa ggtagttgac tgaaactaaa ccagaaaaca gactcgcgat
     5941 cctgcggtaa attattacca gtgtagagca ggcgtaacgc ctgacctgcg gcagcttcca
     6001 tgcgaaatgt ctgcggggtt ggcataaacg gtacattctt gtcagaacca cgttcatcct
     6061 caacatggat attaatgata tagggctgat cgctggtatt tcgcaactgg atggtttttt
     6121 cctttgcgtc accagggaaa ataatacggg ttccggtcat taccacgctg gcaaacgcgc
     6181 tgctgctcat gctcatggaa aatacaagta ccagtaaata tgcaaaaatg ttcattctta
     6241 tcttttccat gtccatgaaa aatcactatt aattgtgcga agaaatactg gctggcattt
     6301 actgccagcc agtagattaa ttagaggtag ctcagggtgt attctgcgac agcggtaatt
     6361 ttaccggcag tggcagcagc gctatcgata acgaagtaac gcgcaccgaa gtcgtagctg
     6421 gcttcggtat cgccaacttt cagggtcaga cccggtacgt tagtcgcgcc agccagagtt
     6481 actgggttac cagaagtgct ggaatccatt aactgaatgc caaagccttt cgccgcatcg
     6541 ctgctggtat cacggtttcc cataacaccc gtgctagcgt caacgtcgta gcccaggaag
     6601 gtggtgttga ttgcctgatc tgcaccagtt ggagcctggc agttagacac agaaaccgta
     6661 aacggcgtct ggcctgcgct ctgaccatca gctaaagttg caccgaagtc agccatggct
     6721 acggtcggca tcaatactac tgaattggtt tgaccgttga tatttacgga acaggtctga
     6781 tcggttactt caccctgaaa agtgactgta ggcgcagcca tagttgaaaa aggtaatgca
     6841 atagctgcca gcagagctgc ttttttgaaa gtagaaaaca tattaatatc cttgaaattt
     6901 agagttaata tagaacgagt taattaatgc aattgataca atctgacgtt tttaaaaccg
     6961 gtaaatctcc ttaactgtat aagtgccttg aaaatgtaaa agccatcact atcacaagca
     7021 atttcaaact tgttaaaaac tgatatttta tagtgactaa tatacttgac atcctttttt
     7081 agcagggtgt gcaatattct tttgttatct atcttaaaat ataagtaata gatatcccac
     7141 tcataccttg tcagagtcac ctgcctgact tgctggtctt tatcagtact tttaatcaaa
     7201 gtaatgagtt tattcaattt tatatatcgg ctaatgaatg tttgctctga aagtgaaaag
     7261 aagatatttc tgtttttcat ctttcttagt gaaacaatca ttaaaaacct ggccaaatct
     7321 ggcagttttg caagtatact tacgtcatcc actgcgacaa gaagtacgtt ttttgtaaag
     7381 cggttgtcaa atctaagtgt acttaggtca ttattgcatg cattttgaga acaaaaaaaa
     7441 cactcaataa aaaaacgata atggtgacct agttttgcac ttaatccctg aaggtaatat
     7501 agatcatccg aaatgataat tacccttgtt agtaagttct tgttcattgc actaactcaa
     7561 ttgcttgcag caaaacagcc catcttcatg gagtaaaaat tttgaaagtg aataaagctt
     7621 tttactaaag atgtaacaat gggcattcca tatgcgacat aacaactgca gtaacacaac
     7681 taatctttgc gtgttcttta tattagagag taacagcgtg atatctgtat ctctaaaaga
     7741 atagcgatgt cctgcaatcg atgagcacaa tatatgacat tcatcacatg tataacaatt
     7801 gaattttcat tcatac
//
LOCUS       DAZKIF010000174         7815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZKIF010000174 DAZKIF010000000
VERSION     DAZKIF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.175"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..406
                     /locus_tag="ACHOTW_004228"
     CDS             <1..406
                     /locus_tag="ACHOTW_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9846227.1"
                     /translation="TLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTW
                     DRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHE
                     DLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(414..680)
                     /locus_tag="ACHOTW_004229"
                     /pseudo
     CDS             complement(414..680)
                     /locus_tag="ACHOTW_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            838..2022
                     /locus_tag="ACHOTW_004230"
     CDS             838..2022
                     /locus_tag="ACHOTW_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP9846228.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNLKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDIEDNSTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            2022..2534
                     /locus_tag="ACHOTW_004231"
     CDS             2022..2534
                     /locus_tag="ACHOTW_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP9846229.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDDESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            2589..2954
                     /locus_tag="ACHOTW_004232"
     CDS             2589..2954
                     /locus_tag="ACHOTW_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9846230.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            2963..3118
                     /locus_tag="ACHOTW_004233"
     CDS             2963..3118
                     /locus_tag="ACHOTW_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP9846231.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3105..5908
                     /locus_tag="ACHOTW_004234"
                     /pseudo
     CDS             3105..5908
                     /locus_tag="ACHOTW_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5921..6409
                     /locus_tag="ACHOTW_004235"
     CDS             5921..6409
                     /locus_tag="ACHOTW_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9846232.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
     gene            6566..7138
                     /locus_tag="ACHOTW_004236"
     CDS             6566..7138
                     /locus_tag="ACHOTW_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP9846233.1"
                     /translation="MLSYLMAIHFVLFGNSTNLKNFWKHEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHA
                     GIVVHFAVDAGENLVLRQNTTIGQIDGDMPSSRVKIGSNVDIGANCCIIGLSRKIGDN
                     VKIGAMSFINKDIPSNCTYITKKSGVVLYK"
     gene            complement(7182..7712)
                     /locus_tag="ACHOTW_004237"
     CDS             complement(7182..7712)
                     /locus_tag="ACHOTW_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9846234.1"
                     /translation="MLAGTNEFIGEGDAYIPPHTGLPANSTDIAPPDIPAGFVAVFNS
                     NEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPDGECQKWNGTSWAKDA
                     EAEKLFRVREAEETKNSLMQVASEHIAPLQDAVDLDIATEEEASLLAAWKTYRVLLNR
                     VDTTVAADVEWPVAPQ"
BASE COUNT         1947 a         1844 c         2168 g         1856 t
ORIGIN      
        1 cacacttgta gaccgaaaat cacgttatac gatcatcctc agactcaggg gcaaagattc
       61 tgtctcagta aatcaggctc ttaccgacaa attcctgagt ttaccgtcag aactcagaaa
      121 atcactgaca tgggacagag gaatggaact ggccagacat ctagaattta ctgtcagcac
      181 cggcgttaaa gtttacttct gcgatcctca gagtccttgg cagcggggaa caaatgagaa
      241 cacaaatggg ctaattcggc agtactttcc taaaaagaca tgtcttgccc aatatactca
      301 acatgaagat ctggttgctg ctcagctaaa caacagaccg agaaagacac tgaagttcaa
      361 aacaccgaaa gagataattg aaaggggtgt tgcattgaca gattgaatct acaagtgcct
      421 ttttctgtca gcggttctcc tttccagcca atgagaaccg tcaggcgcgc gccccgtggc
      481 ggtagctgca actgaccatc tgcatcatcc agcgtgatgg tgagctggtc cgcctcaaat
      541 ccccggttgt cggtcagtga caggctcatc aggcgctctg ccacgcctga cagcgtttta
      601 ccctccgcga gaatatcaaa atccggcact ttcacgtggt ctgtgccctg actgagcaat
      661 tgcatggtgg tgtcggtcat ctgttccctc cctgtgcggc atggtcgcat gtgcgtgcgg
      721 agggggttac tgctttttgt tgtcgccgtg gcgggagaac ggcgcagggg tgagattacg
      781 cgcgtggtgg gtgatgattg ttgccgaatc atttaacgga tacaaggggc tgaagctatg
      841 agtgaaactc gttttcatgg tgcccgtgtt acggaaagta ccgacctggt aacagcgatt
      901 aatgatgttg attccagtgt tatcggtatc gtggcaacgg cagatgatgc ggacgcggag
      961 ctgttcccgc tgaacaagcc cacactgctg acccgcgtca atgacgtgct gggaaaatgc
     1021 gggactacgg ggacgcttta tcgtgcgctt aaggctatcg cagaccaggt gagcacaaaa
     1081 gtgatcgtcg ttcgcgtggc tgaacacaaa gaagaaggcg gtaagacgca ggatcaactg
     1141 gttatcggtg gttctgaatc tgacggcagc tatacgggga tgtatgcgct gcttgttgca
     1201 gagcaggatg aaagcatcgg ataccgtccg cgtattctgg ccgcgccgga gctggacacg
     1261 gaggcggtaa caaaatccct gtgcgtgatt gcgggtaaac tgcgcgcgtt tgtgtatgcc
     1321 acatgtcatg gttgtaacac gatggctgag gcgattacct accgccagaa attcaacgaa
     1381 cgtgaggtga tgctcttatg gcctgacttc atcgcctaca acctgaaaag tggcaaaaac
     1441 gaaacgttcc ccgcgcctgc ttatgcgtgc ggccttcgtg cgtacattga ccatgagcag
     1501 ggctggcaca aatcgctgtc caacgttccg gttaaaaatg tgcttgggat gtcgaggcat
     1561 gtgttctggt cgttgcaggc cgaagacagt gatgccaaca gcctcaacaa caaagaaatc
     1621 acgaccatta ttcgtcgcaa cgggttccgc ttctggggca accgcacacc ggaaacgaac
     1681 gcctacatct ttgaggtgta tacccgaacc gcacaggtgc tggctgattc aattgcggaa
     1741 gcgcagtttg aaaccatcga cagtccactg acacctgcga acgtgaagga tgttatcagt
     1801 gccatcaggg caaaactgga ttcactggtt actgccggga aactgattgg cgcggagtgc
     1861 tggtatgaca tcgaggataa cagcaccacg aatttacgtc aggggcgtgt gcgtattcgc
     1921 tacaaatata cgcccgttcc tccgctggaa gacatggagc tttaccagtc gtttactgat
     1981 gaattctttg gtcccgcatt tgcggtgctg ggaggtgcct gatggctgta ccaaaacatc
     2041 ttcgcttttt tacgctgttt gtggatggtg aaaacgaagt gggtaaggtg acgtccgtca
     2101 ccctgcctaa actgacgcgc aaaaccgaca gctaccgggg tggtggcatg atgggtgcgg
     2161 taagtattga cctcggcctg gacgactccg cgcttgatgc gagctttgtc atggggggcg
     2221 cagttcgtga gctgttcctg aagtatggcg gcacgattga cggcacgcta ctgcgttttg
     2281 cgggggaata ctacaccgat gatgaaagcg acctgtatga agtcgaaatg cgcggacgtg
     2341 tgacggaaat tgatatgggg gaagccaaac agggcgaagc cacatcacac acttacgcca
     2401 ttaaaaacac ctactacaag ctgagtgtta acgatcgccc gttgtgggag attgacctgc
     2461 tgaacttcat ttaccggaag gacggcaagg acattgtgcc cgatcgcatc cgttccgcgc
     2521 ttgggcttgg ctgataagta atatgcaggc ggcgcagtgc gtcgcctctg actgaaagga
     2581 gtttcctgat gaaagagacg aaaaacatcg ataccgaaaa cacggtagtt actgacactg
     2641 tgaaagaaac cagtgagcgt ggtgtaaaac ttacccaacc aattgagcga ggcggcgaaa
     2701 aaatcacgta tgtggagatc accggggcta ttgagcaggc tggatctctg cgagatttgt
     2761 cgctgtctga tgtgctgaat ctgaaagcgg aatccatgtt tacgctgctg tcacgcgtga
     2821 catcaccgcg actggatgaa gtgacgatca aaaaaatggc atcccgtgac tttattcagt
     2881 tatgtgtggt tgccgtaaat tttttgagcg gtgcggactc tggcgggaag aacgaacagg
     2941 cgacggaagc ctgatcacgg ttgtgtgctt tgagcacata gaagactttg tggcagatat
     3001 tgccgttatt tttaactggt cgcccgccga aatcttcatg atgacgcccg gcgaagtggt
     3061 tagctggcgt gagcgggcgg cacttcgcag cgggaatgca gacaatgaag actcttgata
     3121 tccgggtcgc tttcagcgcc gttgacaggc tgacccgacc tgccgaaaac gcacgccgcc
     3181 tgatggggca gtttggtgac tccatccagc gaacgcaggg ggcgatcaaa aatctcgagc
     3241 gtcaggcgcg atcatttgag cgcgcccgcg acgctgtcag taaagcggat gctggcatcg
     3301 tgaaagcacg acgccagctt aacgccctta atcagttaca acgcacgggt acagtgctca
     3361 gcgaaaaaca acaaaagctg atgcagcagt taagcacccg gcttgaacgc ctgaatgaat
     3421 cgcgcacacg ggaaattcag aaaatgaggg aacttggcgg agagctgaaa cgccacggca
     3481 tttccctgac aggcagcgat aacaccattc agcaggccat cagacgcacc gaacaataca
     3541 acaaccagct tgaacgcgaa cggcaggcgc ttgcgcgtgt aacgcaggcg cgtgagcggt
     3601 attcgcacgc gcaggaaacc gcgggaaaac tgaaaacagg tggtgcgctg gcaattggtg
     3661 cggcagcggc gggcggctat gctgccgggc gttttttgca gcctgcgatc gggttcggca
     3721 aagagatgtc ccgcgttcag gcactgacgc gaatcgacaa aaacagcccg cagtttaagg
     3781 cgctgcgtga gcaggcgtta aaacttgctg aaacacagtt tactgcgagt gatgccgcca
     3841 gtgggcagag ctttctggca atggctggtt ttactccgca ggccattcag gccgcattgc
     3901 ccggtgttct taatatggcg ctggcaggtg gcgtcgaact cggcgagacg gctgatatag
     3961 gctccaatat cctcacacag ttcaacctga cagccgatca aatggaccgg gtgggcgata
     4021 cgctgacagc agcatttacc cggaccaata ctgatttacg cgcgctgggc gaaaccatga
     4081 agtataccgg tccggttgcc gcaaaacttg gtatcagtct tgaagaagcg gcggccatgg
     4141 ccgggatgct tgccaataat ggtcttcgcg gaagcgatgc tggcacggcc atgcgcgcaa
     4201 gtctgtcccg ccttgcatca ccgccaaaag ctgcggctga tgcactgaaa gagctggggg
     4261 tgtcagttgc tgacgccaga ggcaaaatgc gcccgatgga ggatgtgctg cttgatctct
     4321 ataaggcgac acaaaaatac ggacaggtgg accaggtctc cttcttcaag gacatcgccg
     4381 gagaagaggc gttcgttggt ttgcagacgc ttgttgcggc ggctggttca ggagagctgc
     4441 aaaaactgac cagagaattg cagggggcaa ggggagaggc cgatcgcgtc gcaaaagtaa
     4501 tggctgataa tcttgatggg gacctgaaaa atctcgacag cgcatgggaa ggtcttcgta
     4561 ttcgcatcag tgatctggtt gacggtccgc tgcgttctgt cacgcagtgg ctcacgcggg
     4621 tgcttgaaaa aatcacctcg ctggcgcagg cccatccggt actgacgcgc cagctactga
     4681 tagcaggcgg tgcgttgctg gcaatgactg caacgattgg ctcgttgtcg ctggttattg
     4741 gggtgcttta cgggaagctg gccaccctgc gtcttggttt tgacattctt acccggtcaa
     4801 tgaatgtcgt cagggtgtta cctgcgctat ggggaatggt gacgggttcc gtttctttac
     4861 tgggaggcgt tatcggggcg ttgttcagtc cggttggtct tatcgtggct gcgcttgccg
     4921 gagctgccgt tcttatctgg aaatactggg atcctatcag ggctttcttt gccggggtgt
     4981 tcagcgggat tatggaaagg ctgaccccgt tgcgcgacac ctttgaacgg tttggccctg
     5041 tttttgacgt aatcggaagc ggttcagcca ggtgtttaac tggtttaaat cgctgctgtc
     5101 accgatggag tccagcaagg aaacgctgga taaatgtacc agtgctggcg aggtattcgg
     5161 taacgttctt ggtggtgcgt tacagcttgt tctgacgcct gcaaaaatgt tgctggatac
     5221 gctggcgtgg atacttgaaa aactcggtgt gcttccggat gaagcggaaa gggcgcgcaa
     5281 gaaaatcgaa gacgcacaac gtgcggccat tcttcaggac aaggttgctc tgtttcaggg
     5341 agacattgcg aaaatcaatc cgccgaagtc tgcgggaaat agcaatggca ccggagatga
     5401 taaacccaaa gacaacaaac ctctcacaga cagcaatacc ggcacgctac gcagactcag
     5461 caaaattgct gataacacag gtaagctggt tgatgagaca aaaaaacgca ttggccccgg
     5521 cgatattgtc tttaagaacc tgccccgcgc acttgctgtt cgtggggagt ggcaggagcg
     5581 gaagattgcg caggtcagta agcctgcccc cgcaattaat atcacacccg tggtcccggc
     5641 tccgctgcct ccggcgctgg tccctgttgt tgcggccagc tcccgcccgg tggtggaggc
     5701 catacgatcg ccagtggcat cagttcctgc aacttcccgt aaccgggagc ctgttgcctc
     5761 cggatttggc ggtgaaattc atgttcatct gcataacgtt gttacgcaga atccccgcga
     5821 actggcgaaa ctggtcggcg aaatggtcag ggcagaaatg gaacggcgcg cccgtgccag
     5881 gcgtggcagt ttttacgata aagattgagg agtcatggcc atgatgatga tctacggcat
     5941 gtttgttttt gagctgcgca cgttgccgca tcagcagtta cagcaaaaca aaagctggcg
     6001 acatgtgaaa aatgaacgcg ttaaccgttc agcaagctgg cagtatatcg gtgcaggtga
     6061 tgatcgcatc gttctttctg gtgttcttta tcctgagatt actggtggcg aagtgtcgct
     6121 gtcgttgttg accacgcagg catatacagg acgtccttgg cctttgattg atggcgtcgg
     6181 gcagatttac ggcatgtatg tcctgaccgg aacgaatacg acccgctccg agtttgatcg
     6241 ctacggtaag gcgaaaaaga tagaattttc actgactctt gaacgctgtg atgaggattt
     6301 gcgggagcgc ctgcaatcct catcgttcag cgatatgctg tccggcttca aagataaggt
     6361 gacatcatcc cttaatagcg cggccagctc agttaagggg ctgctctgat ttaacgtatg
     6421 tcgccaattt cctgatgaag gtgactggcg acttgctgtt gtatgtcctt ctcagaaaat
     6481 tgtttttgaa taacaaataa caggatttta taatctctta accttataac atgtgtggtc
     6541 tgaaataata attaaggaga ttatcgtgct atcttactta atggcaattc actttgtttt
     6601 atttggaaac tctactaatt taaaaaactt ctggaaacat gaagtaattc ggcggaaacg
     6661 tatggatatc tggaggcttt taagagagaa aaagcagcgt aaccggaatt tccttttctg
     6721 gtggcggttg gctaacgaaa tgtatattaa tggtaataaa ttacataaga aagcagccaa
     6781 aaagttaaac agtaaaataa ttaacaagtt tggttgtgaa ataggattgg gcgcaaatat
     6841 tggaaaaggg ttgacaattc cccatcatgc tggaattgtt gttcattttg ctgttgatgc
     6901 gggtgaaaat ctggtattac gacagaatac taccattgga cagatagatg gtgatatgcc
     6961 tagttcaaga gtaaaaattg gtagtaacgt tgatatcgga gctaattgtt gcatcattgg
     7021 attatcacgt aaaattgggg ataatgtaaa aataggtgca atgtctttta taaataaaga
     7081 tataccatca aactgcacat atataactaa aaagagtggt gttgtattgt ataaatagag
     7141 tacataaagc catcgatatt tctgtcgatg gctttttctt tttattgtgg ggcgactggc
     7201 cactcaacat ccgctgctac tgttgtatca acacgattca acaatacccg atatgtcttc
     7261 catgcagcca gtaacgatgc ctcttcctcc gtcgcaatat ccaaatctac ggcatcctga
     7321 agtggcgcaa tatgctcact ggctacctgc atcaggctgt tttttgtttc ttctgcctcc
     7381 cgtacccgaa acagtttttc tgcttctgca tctttcgccc aggatgtgcc gttccacttc
     7441 tgacactctc catccggcga caaccaggta acattttccg gtaacggacc gagttcagaa
     7501 ataaataacg cgtcgccgga agccacgtca taaaccgttt taccccgatg atcttcaacg
     7561 agatgccacg atgcctcatt actgttgaaa acagccacga agccagccgg aatatctggc
     7621 ggtgcaatat cggtactgtt tgctggcaga cctgtatgag gcggaatgta tgcgtcacct
     7681 tcaccaataa attcattagt tccggccagc agattgaacc gccccgggaa tcctggagac
     7741 taaactccct gagaaagagg taaacaggat gactaaaaat acacgttttt cccccgaggt
     7801 ccgtcaacgg gcagt
//
LOCUS       DAZKIF010000175         7760 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZKIF010000175 DAZKIF010000000
VERSION     DAZKIF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.176"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            348..2249
                     /locus_tag="ACHOTW_004238"
     CDS             348..2249
                     /locus_tag="ACHOTW_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9846235.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            2277..2738
                     /locus_tag="ACHOTW_004239"
     CDS             2277..2738
                     /locus_tag="ACHOTW_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP9846236.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            2758..7608
                     /locus_tag="ACHOTW_004240"
     CDS             2758..7608
                     /locus_tag="ACHOTW_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9846237.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSAGRHRISG
                     ETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQLTGYRSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGF
                     ELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTDYPDPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDP
                     LGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLGNRALKYA
                     NGDWIKSEAAFNKYINMINKRLELTGSKFRVEIQPAIKNGERVPATTNGPFKVNGKWT
                     SGTHYTGGSKRLDAGIIDITSPTNQYGLHPVIEGFDITLNKTKPSAVDIYSDVFGGID
                     INDFRL"
     gene            7626..>7760
                     /locus_tag="ACHOTW_004241"
     CDS             7626..>7760
                     /locus_tag="ACHOTW_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846238.1"
                     /translation="MKEIDLLYENIYQLLIKPYLLDLSSQSGKKIELNYTCKIKDAAD
                     E"
BASE COUNT         1920 a         2030 c         2293 g         1517 t
ORIGIN      
        1 ggagcggtag catatatttt ttaagcaatc attcatttta atgatgttaa ttgggttatt
       61 tagttgtgaa taaaaacata ttatgaatat tggtttaact ttgcgttttt gttactcgct
      121 gtgatgtgcg cacctgtcct gaataacgtc aggaaacaat aagttgagct tgtgattatt
      181 tttttgactg aacagacacc cgtttctgta aagtaatatt taaacaaaat cctcacataa
      241 tcatagcatt ttttgttcct tccctaataa cgtcttaagt cgtcccgctt atctcattat
      301 ccgcagttgt acggtgcgga ttattattca ttttaccgga ggttgctatg tcaaccggat
      361 tacgtttcac actggaagtg gacggcctgc caccggacgc ttttgcggtg gtttcctttc
      421 atctcaccca gtcactctct tcgctctttt ccctcgatct ctccttggtc agccagcagt
      481 ttctctccct tgaatttgcg caggtgctgg acaaaatggc ctacctgaca atatggcagg
      541 gtgatgatgt acagcgccgg gtgaaaggtg tggtgacctg gtttgaactg ggggagaacg
      601 acaaaaacca gatgctgtac agcatgaagg tgcacccgcc gctgtggcgt gccggtctgc
      661 gccagaactt ccgtatcttc cagaacgagg acatcaaaag catcctcggc acgatattgc
      721 aggaaaacgg ggtgaccgag tggagtccgc tgttcagtga accgcatcct tcccgtgagt
      781 tttgtgtcca gtacggtgag actgattacg atttcctgtg ccggatggcg gcggaggaag
      841 gcatcttctt ttatgaggag catgcttaca aaagtaccga ccagagcctg gtgctgtgcg
      901 acacggtccg ccatctgccc gaatcttttg aaatcccctg gaacccgaac acccgtaccg
      961 aggtgagcac cctctgcatc agccagtttc gctacagcgc acaaatccgc ccttcttccg
     1021 tggtgaccaa agactacacc tttaaacgcc ccggctgggc aggacgtttt gatcaggaag
     1081 gccagcacca ggattaccag cgcacgcagt atgaggtgta tgactacccc ggacgtttca
     1141 agggcgccca cgggcagaac tttgcccgct ggcagatgga gggctggcga aacaatgcag
     1201 aaaccgcgcg gggaatgagt cgctcgccgg agatatggcc gggacggcga attgtgctga
     1261 cggggcatcc gcaggcgaac ctgaaccggg aatggcaggt ggtggcaagt gaactgcacg
     1321 gcgagcagcc gcaggcggtg ccgggaaggc ggggagcagg gacggcgctg gagaaccatt
     1381 ttgcggtgat cccggcagac cggacatggc gaccccagcc gcggctaaaa ccgctggtgg
     1441 acggtccgca gagcgccgtc gtgacggggc cggagggtga ggaaatcttc tgtgatgaac
     1501 atggccgagt gcgggtgaaa ttcaactggg accgttataa cccggcagac caggacagct
     1561 cgtgctggat ccgtgtggca caggcgtggg caggcaccgg ttttggcaac ctggcgatac
     1621 cgcgtgtggg tcaggaggtg atagtggact tcctcaacgg cgatccggac cagccgatca
     1681 ttatggggcg cacctaccac caggaaaacc gcacccccgg cagcctgccg gggacgaaga
     1741 cgcagatgac catccgttcc aaaacgtata tgggcagcgg gtttaatgag ctgaagtttg
     1801 atgatgcgac agggagagaa caggtctaca tccacgcgca gaagaacatg gataccgaag
     1861 tgctcaacga ccgtaccacc accgtaaaac acgatcatcg cgaaaccgta aaaaatgacc
     1921 agacggtcac gatccaggaa ggtaaccgcc ttcttacggt ggaaaaaggc cacaagatca
     1981 ccggagtact gaaagggtct ttatctgagg atgtctttca ggacagaggc acgattgccg
     2041 gttcggtgca tgttgacgct gtaaacaatg gtggcgaagg caacggtata caggcttata
     2101 cggcaattaa ggaaattatg ctggccgtgg aggaaagcaa aattgcgctg acgccggatg
     2161 gcattcagct acaggtcggg gaatcaacgg taatcaggct gtcgaaggat ggcatcacca
     2221 tcgtgggcgg ttctgttttc atcaactgat tcactcagat taacagggag tattttatgc
     2281 agttcacttt taatgaaggt catattcaac tgccatcgca atggcaggat cagtcgatgc
     2341 aggtcctggt atccacggat aacagtggca tcaacctggt cattacccgc gaacccgttt
     2401 cacagggaac cctgacacca gagctgtacc aggaaacgct ggcgctgtac cagggcaaac
     2461 ttgatgggta caccgaacat gcctgccggg aaatcactct ggcagaggcc ccggcctggc
     2521 tgctggatta ttcctggaac ggtccggaag atgaagggaa tcaggggcgg attagccaga
     2581 tagccgtatt ccagcggcgt ggagatacgc tgctgacctt cactttcagc acctccttat
     2641 ccctgaagaa cagtcaaaag acgatgctgc tggaggtgat aaagagcttc accccgttgc
     2701 caccggagaa tgacatccaa aaggaccagc cgcgctgaca taaacgaggg ataaaacatg
     2761 agtgaaggac caggcgggcc acagggagcg accgcaggcg gtacgctggc aatgcggatg
     2821 ctgtcgcagc gggcgatggc cgccagccag atgaaacggg cagccaacga caaagccatt
     2881 gcacagatgt tggcatcaaa aaagtctggc ccccccgccg ccaggctggg cgatgaaatt
     2941 cagcacaaga gttttttggg ggcgctggca ggggccgtgc tgggggcgat agtgaccata
     3001 gcagaaggtt gcctgattat ggccgcctgt gccaccggcc cttatgcgct ggttctggtg
     3061 cctgcgctga tgtatgccag ctataaggcg agtgattatg tggaggagaa acagaaccag
     3121 cttgaatcat ggataaacag cttttgtgac acggacggcg ccatcaatac cggttctaaa
     3181 aatgtaaaaa ttaacggaaa gccagcagcc cgtgcagccg tcacccttcc ccctcctccc
     3241 ccacctggag caatacctga agtcccacag ggggaaccct catggggtga tattgccact
     3301 gacctgcttg aatcggcagc ggaaaaagca gtaccactgg cgaaggcctg ggggaacgct
     3361 gttatcaccc tgacggacag caatgccggt tttatggatc gcgtcagcgc cggcgcatcg
     3421 cttctgtttc ccgccggtcc ggtattaatg gagtttgcca ccatggtggg cgggcgtggc
     3481 gaaatcaaaa aagatgtgga tttcccggaa gccggtgagg acacggcgct ctgcgacaag
     3541 gagaacaaac caccgaggat agcccagggc agcagcaacg tctttatcaa caatcagcct
     3601 gccgcgcgca agggcgacaa actggagtgc agcgcggcaa tcgtggaagg ttcgccggac
     3661 gtctttattg ggggtgagca ggtcacctat ctggatatcc agccggagtt cccgccatgg
     3721 cagagaatga tcctgggagg aataacgata gccagctatc ttctgccgcc agcaggactg
     3781 ctgggaaaac tggggaatct ggcgaaactg ggcaaactgg gaaacctgct ggggaaaagc
     3841 ggtaagctgc tgggcgcaaa gctcggcgcg ttgctgggga aaacaggtaa ttcgttaaaa
     3901 agtattgcca ataaagtcat cagatgggta acagatcctg tcgatccggt aaccggcgca
     3961 tactgcgacg aacgtaccga ctttacgctg ggccagacac tccccctctc cttcacccgt
     4021 ttccacagtt ctgtactgcc gctgcatggc ctgacgggcg tgggctggag cgactcctgg
     4081 agcgaatacg cctgggtgcg tgaacaggga aaccgggtgg atgtcatcag cctgggagcc
     4141 acgctgaact tcgccttcga cggtgaaagt gatacggcgg ttaacccgta tcacgcccag
     4201 tacattctgc gccgccgtga tgattatctg gagctgttcg acagggatgc actgagcagc
     4261 cgcttctttt atgacgcctt tccgggaatg cgtctgcgcc acccggtgac tgacgatacc
     4321 agcgatgacc gcctggcaca cagccccgca gaccggatgt acatgctggg cgggatgagc
     4381 gacaccgcca gcaaccgcat cacgtttgag cgcgacaccc agtaccggat cacgggtgtc
     4441 agtcacaccg acgggatccg gcttaaactg acgtaccacg ccagcggcta cctgaaagcc
     4501 attcaccgca cggataacgg catacagacg ctggcgacct acgaacagga tgcgcggctg
     4561 gactaccacc tgttttatga gtacgacgct gcggaccgga tcatccgctg gtccgataac
     4621 gaccagacgt ggagccgttt cacctacgat gcacagggcc ggtgcgtgac cgtcaccggg
     4681 gcggagggct attacaacgc cacgctggac tatggtgacg gctgcaccac cgtgacggac
     4741 ggcaagggca ttcaccgtta ttactatgat cctgacggca atattctgcg ggaagaagcg
     4801 ccggacggca gcaccaccac gtatgaatgg gatgaattcc atcacctgct ggcccgccac
     4861 tcccctgccg ggcgggtgga gaagtttgaa tacaacgccg cacacggtca gttaagccgt
     4921 tacacggcgg cagacggcgc ggattggcag tactgctatg atgagcgcgg cctgctcagc
     4981 aacatcaccg cccctgccgg gcagacgtgg acgcagcagt gtgatgaacg cggcctgccg
     5041 gtgagtctgg tatcgccaca gggcgaagag acccggctgg cgtacacccc tcaggggctg
     5101 ctgtcgggga tattccgcca ggatgaacgg cgtctgggca tagagtacga ccaccacaac
     5161 tggccggaaa cactcaccga cgtgatgggc cgcgaacacc acaccgaata cagcggtcac
     5221 gacctgccgg tgaagatgcg cggccccggc ggtcagtcag tgcggttgca gtggcagcag
     5281 caccataaac tgagtggcct tgagcgggca ggaaccggcg cggaaggatt ccgctacgac
     5341 cgccacggca acctgctggc gtacacggac ggtaacggcg ttgtctggac aatggagtac
     5401 ggcccgtttg atttgccggt ggcgcgaacg gacggtgaag gccaccgctg gcagtaccgc
     5461 tacgataaag acacgctgca actgacagaa gtcattaacc cgcagggcga gtcttatctt
     5521 tatattctgg acaactgtgg ccgggtgacg gaagaacgtg actggggcgg cgtggtctgt
     5581 cgttaccgtt atgacgctga tggcctgtgt accgccaggg tcaacggcct agaggaaacc
     5641 atcctctaca gccgggatgc cgcaggccgc ctggcagaag tcatcactcc ggaaggcaaa
     5701 acgcagtatg cgtatgacaa atccggcagg ctgacgggta tcttcagccc ggacggcaca
     5761 tcacagcgca ccggctatga tgaacgcggg cgggtgaatg tcaccactca gggccgacgg
     5821 gccattgaat accactaccc cgacgaacac accgtcatcc gctgtatcct gccaccggaa
     5881 gatgaacgcg acagacaccc cgacggatcc ctgctgaaaa ccacataccg ctacaacgcc
     5941 gccggagaac tgacggaggt tatcctgccg ggggatgaga cgctgacgtt cagccgtgat
     6001 gaggcgggac gtgaagtgct ccggcacagt aaccggggtt ttgcctgtga acagggctgg
     6061 aatgcagccg gtcagcctgt cagccagcgc gccggacttt tcccggcgga agccacatgg
     6121 ggcggactgc tcccttcact gctacgggaa taccgttacg acagcgcggg taacgtatca
     6181 ggcgtcacca gccgggaaga ttacggacgg gaaacacacc gggagtaccg gcttgaccgg
     6241 aacggccagg tcacggcggt gacagcctca ggcaccgggc tgggctatgg cgaaggcgac
     6301 gagacttatg gctatgacag ctgcggctac ctgaaggcgc agtctgcggg cagacaccgg
     6361 ataagcggag agactgacca gtatgccgca ggccaccggc tgaaacaggc cggaaacaca
     6421 cagtatgact atgacgccgc aggccggatg gtcagccgca caaaacaccg tgacggctac
     6481 cgcccagaaa cagagcggtt ccggtgggac agccgggacc agctgaccgg gtatcgcagc
     6541 gcacaggggg agcagtggga ataccgccac gacgccagcg gcagacggac ggaaaaacgc
     6601 tgcgaccgga agaaaatccg ttttacgtac ctgtgggacg gcgacagtat tgcggaaatc
     6661 cgggaatacc gcgatgataa actgtacagc gtaaggcacc tggtgtttaa cggctttgag
     6721 ctgataagcc agcagttcag ccgggtacga cagccgcatc cgtccgtggc cccgcagtgg
     6781 gtgacgcgaa cgaatcatgc ggtgagcgac ctgacgggcc gcccgctgat gctctttaac
     6841 agtgaaggta aaaccgtctg gcggccgggg cagaccagcc tgtgggggct ggcactcagc
     6901 ctgcccgcag acacagacta cccggacccg cgcggggagc gggacccgga agcggacccc
     6961 ggcctgctgt atgcgggaca gtggcaggat gcagaatcgg ggctgtgcta taaccggttc
     7021 cggtactacg agccggaaac cgggatgtac ctggtgagtg atccgctggg gttgcaggga
     7081 ggggagcaga cttatcggta tgtgccgaat ccttgtgggt atatcgatcc tttggggctg
     7141 gctatatgtc agttagcccg ctggacgaaa tgggggagtg agcaaagcaa catatctgat
     7201 gttttgaact cattagggaa tagagcactt aaatatgcta atggtgattg gataaaatca
     7261 gaggctgcat tcaataaata cataaacatg ataaataaaa gactagaatt aacaggtagt
     7321 aaatttagag ttgagattca accagccata aaaaatggag agcgagttcc tgcgacaacg
     7381 aatggaccat ttaaagtaaa tggtaagtgg acatccggca ctcattatac aggtggttcc
     7441 aaacgtctag atgccggtat tattgatatc acatctccta caaaccaata tggattacat
     7501 ccagttattg aaggatttga tataacactt aataaaacaa aaccatcagc agtggatata
     7561 tattcagatg tgtttggtgg gattgatatt aacgactttc ggttataaaa caataaggcg
     7621 gcaatatgaa agagattgat ttattatatg aaaatattta tcagctttta attaaacctt
     7681 acctactcga tctttctagc cagtctggaa aaaaaataga attaaattac acatgcaaaa
     7741 taaaagatgc tgctgatgaa
//
LOCUS       DAZKIF010000176         7702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZKIF010000176 DAZKIF010000000
VERSION     DAZKIF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.177"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            91..827
                     /locus_tag="ACHOTW_004242"
                     /pseudo
     CDS             91..827
                     /locus_tag="ACHOTW_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1006..1302
                     /locus_tag="ACHOTW_004243"
     CDS             1006..1302
                     /locus_tag="ACHOTW_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9846239.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1610..1726
                     /locus_tag="ACHOTW_004244"
     CDS             1610..1726
                     /locus_tag="ACHOTW_004244"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846240.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1945..3330
                     /locus_tag="ACHOTW_004245"
     CDS             1945..3330
                     /locus_tag="ACHOTW_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP9846241.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIMIN
                     ELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGPLRHCREYAAKNAEYMA
                     RLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPLTFDNSALTVPVRLIPE
                     ELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWLLDLLYEQNRVVPVIEG
                     KTLCVAPNFDL"
     gene            4151..4714
                     /locus_tag="ACHOTW_004246"
     CDS             4151..4714
                     /locus_tag="ACHOTW_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9846242.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            4869..7229
                     /locus_tag="ACHOTW_004247"
     CDS             4869..7229
                     /locus_tag="ACHOTW_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9846243.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(7391..>7702)
                     /locus_tag="ACHOTW_004248"
     CDS             complement(7391..>7702)
                     /locus_tag="ACHOTW_004248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846244.1"
                     /translation="DYHYKKAAIEYVAQMLISNIGNLEEYTFVPVPPSKCRTDAAYDD
                     RMTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMANYQLDEQLLEGCR
                     P"
BASE COUNT         2155 a         1522 c         1848 g         2177 t
ORIGIN      
        1 gtacacgtat taacagatta tgtcagaagg ccccggtgat gccttctgac gatagattaa
       61 tgttgtattc tattggtatc tggagaccat atggcaacag ttacagtata ttctccccgc
      121 tgtaattcct ataaagtgta ccgttatgat cacagcacct cagatcatga gcgtttccag
      181 tgctaatcct gcaaacgtgt atttcaactg aattacatct acgaaacccg aaaaccaggc
      241 attaaagagc aaattgttga aatggttcat aacggagccg gggttcgcga tagctccaga
      301 acgctgaagg tggatatcaa caccgtcatt ctcactttaa aaaacgctca ccacgtaaag
      361 taaccatatc tccagccgcc agtgtcgatg tggcacttat ctgtgaactt gatgaacaat
      421 ggagttttgt cgaaaacaaa gctcgtcagc agtggcactg gtacgcgtat aagaccaaag
      481 ctgacggtgt gctggcttac acttttggtc ctcgcactga tgaaacatgc cgtgagttgc
      541 cggaattcct gaagccattc tccgcaggca tgataaccag agacaaccgg agcagttata
      601 ccagagaaat gccacaggat aagcatcttg tcggcaagat atttacccgg cgcattgagc
      661 gcaacaatct gacacttcgt actcacatca aacgacctgc ccggaaaacg atttgctttt
      721 tacgctccct ggaaattcac gaaaagccat tggttcattt atcgaaaaca catgtactac
      781 tgacaggagt cattaccagg gcctcttttg ctgttttttt gccatgaatg ataatgagaa
      841 tcgacagcga taagggaaaa gtaaaataac actaatgctg tcgttttcta tcatattgat
      901 ttttaattat ttattgtctt ttttctcaac ccattttttt acgcagacaa atttacccgg
      961 ggaatacaaa ctgtaacgac agttcaccac actatggggg ataaaatgcc agatcaatcc
     1021 ggacactgca ggactccttc acgcatctta cggatgcctg aactgagcca attgttaggt
     1081 atatctcgtt cgacaattta cgaaaaaatg aatccgttat cgaaatacta cgatgcaacc
     1141 ttccccaggc cggtacgtct tggtagtggt tctgtgggct ggcgttcatc agctattgac
     1201 gaatggctta ctttacatac tgtacctgcc agatcagcag tcaaaggtgt taatgacgaa
     1261 gtgagacata cgctttcttc agatatgacc atgagcagat aagtttttgt catcacgtga
     1321 tcacctcagg aggaaaaaga agcataagaa caatttagcc agaagtttaa ttgataaaca
     1381 gaatttccag cattcattgt tgaaagagca gctattgtcg ctattgtagc ggtaattgct
     1441 gctataaccg aaacgtatta cgaaaaagga agcatttatg ccttacatca aaccggatat
     1501 gtcgtattca gtatatgcat gaccatcaat gatagggaag gcaataatta aagctcaata
     1561 caataaacaa gtacctctga ctctggttgc aatgtcagta ttaacagcga tgtcaattgc
     1621 ctgtcagaat caggttgacg tatgcagtcc cggaaattta cgtggggccg ttaatattta
     1681 cactatggtt ctggagaaag ggaaaagacc gggggaaagg tattagtgaa ataatttccg
     1741 gaggtattaa gaatgagaaa tatgaatgat atttcgtggt gaataataca gtcttcactg
     1801 aattaacctt atcggtttat taaacatcag atctacaaat gctctgtatt tctcagagca
     1861 tcaggtctga atagcagcta ttttcgctat tgtagcggga tttgctgcta ttttactaac
     1921 ccattaatga aaagagaggc ttttatgtgc ttacttgcac cagaaaatcc ttatccgata
     1981 tatgcactac caccgctggt gagaaatgca ataattgaaa ctcaaaaaaa tacacaggca
     2041 cctttggcta tggtggcaac atccgcatta acagcgatct caattgcctg tcagaatcag
     2101 attgacgtgt gcagacctgg aaatttacat gggcctgtta atctttactc tctgattctg
     2161 gctgattccg gtgaaaggaa aacgaccgtg gataaagtgt ttatgaaagc attttatctc
     2221 agggatgaag ccctggcgga tgaatacgcg aaactggttg agaattacag tacagaaaag
     2281 gaaatatggg agcaaaaaca aaaagcgctg gaatcaaaat ttcataaaga gattcgtgcc
     2341 ggtaaagatt acaaggcaac agaatcagag cttgaaacgc atctgaataa acctcctgtt
     2401 ccgccgcaga tacgtcgaac aatttttaat gagacaacga tagagggaat gttaaaatat
     2461 tactccgata gcaatcgctc ttttgctctt gtatccagtg aagggggggt aatttttgac
     2521 agccgggcca tgagtaaact gggaattatt aacactttgt gggatggagg ttctcttttc
     2581 atcgacagga aatcatctcc cggaattaat ttgaaggagc caagactgac gatatcggtg
     2641 atgattcagc ctgatgttta ccacaaaggt ttttgtacgc gaaaaaaaga aattgtgaaa
     2701 acgtcaggac atcatgcaag gtttttgatg tgtcaaccaa catcaacgca ggggacaagg
     2761 ataatgatca atgagcttat tgatgaaagt ctggcgatga gtggtgaacg acgttgcctt
     2821 cacttttctc ctcaggctgc cagaatctgg acggattatt acaatgatgt ggaatcgaag
     2881 ctggggggat tggggccttt aagacattgc cgggaatatg ctgctaaaaa tgcagagtat
     2941 atggcaagac tggcaggact tatttaccat tcgagcggtg aagaggggga gatttcccct
     3001 tacactgcag aaatggcgag agaattagca atatggtacg gtaatgagta tgtgcggttg
     3061 tctaatccgt taacttttga caactctgcc ctgaccgtac ctgtgcgact cattccagag
     3121 gagcttgaac ttttcaactg gataaaaagc tattgtattg agaaggggat cctctgtatg
     3181 aaaaaaaatg acattttaca gcgtggtccg aaccgtttcc ggaaaaagga taaaatcaac
     3241 tggttactgg atttactgta tgaacaaaac agagttgtac cggttattga gggaaaaacg
     3301 ttgtgtgtcg cacctaactt tgacctctga acgacttaat cgttgagtac atatagtttc
     3361 ctctgctact tgcggaggag actaatgtct ttaccatgtc agcgttgtat tagaaaactc
     3421 tggtacaggt cagtcagtaa atataactgg tgtaaccact atttactatt ggatagagtg
     3481 ttgctcagga tattgtcggc tttagtctga ggtgaggtgg ggccttcatt gttttcgtgt
     3541 tcaataaata atttaggtta attagtatta ttagtggaag ttatgtgatc tgttatgttg
     3601 tcttgtacgc acttaacact ggttacgtac cggaacacta ttagagtatg ttatggctct
     3661 gttacagttt ataggtatga aaaaatataa tctttcctgt atgaaataat attcttatgg
     3721 tgtgtggggt atggtctgta taccatcatt tttatggtga agttaaatat ctgtagaggc
     3781 cttaagctgg gtcgctgttt ttacttaatg gtacagaagt attaatagcg gtgttgcctg
     3841 tggctacccg atactggctt ttgtgaatgt cagattactt cctgtaagtg attgaatggt
     3901 acttccttaa actcaattct atgaggtgtg tttttaccat tgtaattcgt actggtcctc
     3961 agtttaagtg agtcttactg tactctgaaa tattacctct ttggagtaat gaggtaatac
     4021 gaaacagttt tatcaggttg tctgtgctgc tggttacctc gtttattaat gccgcggtac
     4081 ggcgtttctg tactctcagt atttatgtaa ttccaccttc ctgtaacttt tataaagtaa
     4141 ggactataaa atgtataatg ctaatcccaa ttacgaaatg gacttcatga ttctgaaaga
     4201 cgtgaatgaa cacatggaag gtctgtttca acgtttttct aaactgttac ctttcagaat
     4261 tgattttgcc tacaggaaag acacgcccag ttttggtcat tcctgtaggc acagtatgtg
     4321 tattgaaatg caccgcttac ttagtgaaac ccaaacgatg ctggcgggat attactgggt
     4381 aatggaatat acgtcaaaca agggacttca tattcatttt atcggctacc tggatgggca
     4441 acgtcacaac aaatcttatc ggatatccag gcaactggga gatatctgga ggcggatcac
     4501 ggaaggggag ggttattttc acctgtgcag ggctaaagac aaatacccgg tgcgtatcga
     4561 tcacgtaatt cactatagtg ataaatctgc ggtagacgac ttacgttacg cccttagcta
     4621 tctggccaaa caggatcaga aagaacacgg tattattctg aggcgtagca ggcttcccga
     4681 aaaaagtaac cggggccgac cacgacataa ctgaattaca gctgtattag gtattagaat
     4741 taagtttctg taagttgttg ggaggtgctt gagttaatag gcagaagagg gcggggcgct
     4801 ttgtcatcag tgtaggggac ccgaatttct gccgtggtgc ctcagtggat cggatacagg
     4861 taacgaatat ggttcataaa tctgacagtg atgaattagc tgcattgagg gcagaaaatg
     4921 cccgtctggt ctcattactt gaagctcatg ggattgaatg gcgacgtaaa ccgcagactc
     4981 ctgtgcagcg cgtttctgta ttatccaccg acgagaaggt tgcattattt cgtcggttgt
     5041 ttcgtgggcg tgatgatgta tgggcactta gatgggaaag taaaaccagc ggcaaatcag
     5101 ggtactctcc tgcctgcgct aacgaatggc aggcgagaat atgcggtaaa ccccggataa
     5161 aatgtgggga ctgcgctcac cgtcagttga ttcctgtatc cgatctcgtc atctaccacc
     5221 atctggccgg tacccatact gtcgggatgt atccgttgct ggaagatgat tcctgttatt
     5281 ttctggcagt tgattttgat gaagctgaat ggcagaagga tgcatccgca ttcatgcgat
     5341 cctgtgatga gctgggtgta cctgctgcgc tggaaatatc ccgttcacgt cagggggcgc
     5401 atgtctggat attttttgcc tcacgagttt cggcccgcga agctcgccgt ctggggactg
     5461 ctattattag ctatacgtgt agtcggaccc gacagctgcg attggggtct tatgaccgat
     5521 tatttcctaa tcaggatact atgccaaaag ggggatttgg taatctcata gcgttacctc
     5581 tgcaaaaaag accgcgtgaa ttggggggaa gcgtttttgt tgatatgaat ctccagcctt
     5641 atcctgatca gtgggctttt cttgtatcgg tgaccccgat gaatgtgcag gatattgaac
     5701 cgacgatatt acgggctaca gggagtatcc atcctctgga tgtgaatttt atcaacgaag
     5761 aagacctggg tacgccgtgg gaagagaaaa aatcatcagg aaacagactg aatatttcta
     5821 ttgcagaacc gctgaaaatc acgctggcaa accagatcta tttcgaaaaa gcgcaattac
     5881 ctcaggtgct gattaaccga cttattcggc tggcagcatt tccgaaccct gagttttata
     5941 aggctcaggc aatgcgtatg tcagtctgga ataagccccg tgttataggt tgtgcggaga
     6001 attacccgca acacattgcg ttgccccggg gatgtctgga cagcgtatta tctttcctta
     6061 gggacaacaa tattgctgca gaattaatcg ataaacgatt tgccgggacg gaatgtaatg
     6121 ccgtttttat gggaaacctc agagcggagc aggaagaggc cgtttcggca ttactccgtt
     6181 atgacactgg tgtgctttgt gcgccaacgg cttttggtaa gacagttacc gcagcggcag
     6241 tgattgccaa gcggaaagtg aatacactga tactggtaca ccggactgaa ttgctgaagc
     6301 agtggcagga gcgtctcgcg gtgtttctgc aggccggtga cagtattggt attatcgggg
     6361 gaggtaaaca taaaccctgt ggcaatattg atattgcggt ggtgcagtcc atatccagac
     6421 aaggagaagt tgaacctctg gtcaggaatt atgggcaaat cattgtggat gagtgccatc
     6481 atattggcgc ggtttcattt tctgcgattc tgaaggaaac gaatgccaga tatctgcttg
     6541 gcctgacggc aacaccaatc cgacgggatg gtctgcatcc cattattttt atgtactgtg
     6601 gtgccattcg ccatacagcg gtccgcccga aggaaagccc acataatctg gaggtactga
     6661 tccgttcccg ttttacatct ggtcatttac catcggatgc gagaatccag gatattttca
     6721 gagaaattgc tctggatcat gacagaacgg tggcgatagc tgaagaagcc atgaaagctt
     6781 tcgggcaggg gcgaaaagtt ctggtactga ctgaacgtac agatcatctg gatgagatag
     6841 catcagtgat gaattcactg aaattgtctc cctttattct ccatggtcga ctatcgaaaa
     6901 aaaagcgtgc gatgctgata tccgggctga atgctcttcc tcccgattct cctcgaattt
     6961 tgttgtcaac aggcagactt attggtgagg gatttgacca ccctccgctg gatacgctga
     7021 ttcttgccat gcctgtgtca tggaaaggga cattacagca gtatgcaggg cgtcttcaca
     7081 gagagcatac cggtaaaagc gatgtcagga tcattgattt tgtggatacc gcgtatcctg
     7141 tgctgctcag aatgtgggat aaacgtcagc ggggttataa agcgatgggg tacaggatta
     7201 tagctgacgg cgatgaatca gtgatttaat tttaaggagt actttaccct ttttaaataa
     7261 tttgagctcc ccgggttgtt cgggcaacaa agagtcccag tatacaggct tcaggaatat
     7321 gttgtagtat caggcttttc atcgctttaa aatgactacc ggcagtgaga acgtcatcaa
     7381 aaataacaat ctagggcctg cacccttcca gtaattgttc atccagttgg tagtttgcca
     7441 taatcgcttc tggtaagggc cggtttgttg cggtatgaga ggctattgtt gatacgtttt
     7501 gcacaaccag ttcccagaaa tccagccatg gttcgtaggt ttgcgcaatt cgcagaattt
     7561 ctgtcatacg gtcatcgtaa gcggcatctg tacggcattt ggatggtggt acgggaacaa
     7621 atgtgtattc ctcaagatta ccgatatttg atatgagcat ctgagcaaca tactcaatag
     7681 cagccttttt atagtggtaa tc
//
LOCUS       DAZKIF010000177         7695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZKIF010000177 DAZKIF010000000
VERSION     DAZKIF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.178"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..814
                     /gene="gadX"
                     /locus_tag="ACHOTW_004249"
     CDS             <1..814
                     /gene="gadX"
                     /locus_tag="ACHOTW_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP9846245.1"
                     /translation="HGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCV
                     ENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKNL
                     RYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARI
                     ASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVS
                     YFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
     gene            1182..1910
                     /gene="gadW"
                     /locus_tag="ACHOTW_004250"
     CDS             1182..1910
                     /gene="gadW"
                     /locus_tag="ACHOTW_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP9846246.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2273..5386)
                     /gene="mdtF"
                     /locus_tag="ACHOTW_004251"
     CDS             complement(2273..5386)
                     /gene="mdtF"
                     /locus_tag="ACHOTW_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP9846247.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5411..6520)
                     /gene="mdtE"
                     /locus_tag="ACHOTW_004252"
     CDS             complement(5411..6520)
                     /gene="mdtE"
                     /locus_tag="ACHOTW_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP9846248.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6628..6906
                     /locus_tag="ACHOTW_004253"
     CDS             6628..6906
                     /locus_tag="ACHOTW_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846249.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6907..7434)
                     /gene="gadE"
                     /locus_tag="ACHOTW_004254"
     CDS             complement(6907..7434)
                     /gene="gadE"
                     /locus_tag="ACHOTW_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP9846250.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1982 a         1806 c         1837 g         2070 t
ORIGIN      
        1 acatgggaat tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg
       61 tgaatatcgc tattttaatg gcggtgacct ggtttttgcg gatgcaagcc aaattcgagt
      121 agataagtgt gttgaaaatt ttgtattagt atcaagggat acgctttcat tatttctccc
      181 gatgctcaag gaggaggcat taaatcttca tgcacataaa aaagtttctt cattactcgt
      241 tcatcactgt agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa
      301 gaatcttcgc tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt
      361 ttttcttgag gatgatcact ttataccgct gcttctgaac gttttacaac cgaacatgcg
      421 aacacgagtt tgtacggtta tcaataataa tatcgctcat gagtggacac tagcccgaat
      481 cgccagcgag ctgttgatga gtccaagtct gttaaagaaa aaattacgcg aagaagagac
      541 atcatattca cagttgctta ctgagtgtag aatgcaacgt gctttgcaac ttattgttat
      601 acatggtttt tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt
      661 catttacgtc tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc
      721 gcagggattg ccgaaccgtg actcggcggc aagtattgtt gcgcaaggga atttttacgg
      781 cactgactgt tctgcggaag gaataagatt atagagtttt actcagacat aaaaaaaacc
      841 cggcataggg gaccgggaag aggatagtct gccgtctcca gactaataaa ccgttataac
      901 actccctgtt ggcacgggaa actttgtgct ctcagtaagt taaatataac ttttactgga
      961 aataagatca gccatttttt tataaacata agctatacgc tgtgcgaaaa tatattcgtg
     1021 ttgcatttac ttattatcaa ttaactgtta tgcaaaacta ctttgtggat aaattttggt
     1081 cctaccaaat ttggcagttt ttgcgctaag aaacagtctg gcatcatttc attagtatac
     1141 tgaaattgaa ataatcgcag tatgaaatat aagggataat catgactcat gtctgctcgg
     1201 tgatcctcat tcgtcgttca ttcgatattt atcatgaaca gcaaaaaata tcgctgcata
     1261 acgagagtat cctgctgctg gagaaaaatt tggcagacga ttttgcgttt tgttcaccgg
     1321 atacgcgacg actggatatc gatgagctga cagtttgcca ttacttacaa aatattcgtc
     1381 agctaccacg caatttaggg ttacatagca aagaccgtct gttaattaac cagtcacccc
     1441 ccatgccgct ggtgacggcg atttttgata gcttcaatga atccggggta aattcaccga
     1501 tactgagcaa tatgctctac ctttcctgtt tatcgatgtt ttctcataag aaagaactga
     1561 tccccttact tttcaatagc atcagcactg tttcaggaaa agttgaacgc cttattagct
     1621 ttgatatcgc caaacgttgg tatctgcgcg atatcgcgga aagaatgtat accagcgaga
     1681 gtctcatcaa aaaaaagttg caggatgaaa acacctgttt cagtaaaata ttactcgcct
     1741 ccaggatgtc gatggccaga cgattactcg agttacgtca aattcctctg catactattg
     1801 cggaaaaatg tggctatagc agtacgtcgt actttataaa cacatttcga caatattatg
     1861 gtgtaacgcc acatcagttt gcgcaacatt cgccaggtac cttttcctga catattttgc
     1921 atttgaatat tggtcaggat ctcacacctg cttcatgtga aactcttccc tgatgatttc
     1981 tgccgggcta ccggctagtt ctctttcgca gtttgccagg cggtataggt attttcagga
     2041 ggaaaagact cggcatgttt gggattatta agctgacaat tcatacgatt acgggaatgt
     2101 gggtcagtat tgtgctgttc aaattgatga cgaatggctg gagtggtttc tactttcagt
     2161 gctgtgtact aagcctggta ttcctgacgg ttagctggtt gttgagtggt gaatggttag
     2221 caggaaagag taaggctgaa ccttcacgtt caaccttact ctcatttaca cgttacgctt
     2281 ttttaaagcg ggcaaagaga tgttccacta caacgaaaaa gaccggaaca aagtaaattg
     2341 ccagcactgt tgcggcaaac atcccgccca tcacgccggt acctaccgcg ttttgcgcgc
     2401 cagaaccggc accatgactg ataaccagcg gcagcacgcc gagaataaag gccagagagg
     2461 tcatcaggat tgggcgtaaa cgcatccgcg ccgcttcgat gattgcctct atcggcgttt
     2521 tcccttcttt ctgcatcatc tcaacggcaa attcgacaat caggatggcg ttcttggcgg
     2581 aaagcccgat ggtggtcagc aaaccaacct ggaagtagac atcattactt aagccgcgca
     2641 gatcggtggc cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc accgagaacg
     2701 gaattgacca gctctcataa agtgcggcga gggcgaggaa caccacgacc agtgaaatcg
     2761 catacagcgc aggagcctga tttgaggata acgcttcctg atacgacagt ccggtccatg
     2821 agtagccgac gcctgccgga agtttagcga ccaggtctgc cataaatttc atggcgtcac
     2881 cggtactttt cccggccgcc gcttcaccta aaatctccat tgacgggatg ccgttgtagc
     2941 gttccagtcg cggtgaacca taggtccatt cggtagacga gtaggcagaa agcggtgcca
     3001 tcgtgccaga ggcgttgcgt acataccatt ggttgatgtt atccggcaac atacggaacg
     3061 gcgtgcctgc ctggacatac acttttttca cccgcccctg gttgaggaag tcgttcacgt
     3121 agctgctgcc gaaggcggtg gaaattgtct ggttgatatc agacagcgcc acacccattg
     3181 cctcagcttt cgcggcgttg acgttcactt tgaacatcgg cgtatcttcc aggccgttcg
     3241 ggcgtacccc ggtgacctga ttcggtgatt gcgctgccag tgataacagc tcgtttcgtg
     3301 cctgggttag tttttcgtgc cccaggttac cgttgtccag cagttccata tcaaaacctg
     3361 acgcggtacc cagttcagcc accgcgggta agttgaacgg gaagacgacg gctttattga
     3421 tactgcttag cgcaatcatt gcccgctgaa tgatcgcggt aaccgagttt tcctcaccga
     3481 cacgttcaga ccacggcttg agactgataa acgccagacc gttgttttgc ccctggccgc
     3541 tgaagccaaa gccgccaacg gtaaacaccg actgaacatt atctttctct tttgtaagat
     3601 aataatccgt cacctgttgc agcactttcg tggtgttaac catggtggca ccggaaggta
     3661 actgcgcggt ggtcataaat accccctgat cctcttctgg taagaaagag gtcggcgtgc
     3721 gcaggaacag caccgccatc ccggcgcaaa tcagcaggta gatcaccatg tagcgaccgg
     3781 tacaacgcaa cagtgagcgg gtgctatcgg tatagtgctg agttgatttt tcaaacagcg
     3841 tgttgaagcg tgcgaacagg gcattaggtt tgtgaccgcc ttccggcgcg gctttcagaa
     3901 tggtggcgca cagggcaggg gtcaggctca ttgccacaaa tactgaaagc agcatggagg
     3961 agatcagcgt gatggagaac tggcggtaga tctccccggt tgcaccgctc ataaaggcca
     4021 tcggcataaa cactgcggaa agaacaacgg caataccgac cagcgcacgt tggatctgcc
     4081 ccatcgattt atgcgtcgct tccttcggtg gtagcttatc ttccgcaatg acacgctcga
     4141 cgttctccac caccacgatg gcgtcatcca ccagtaaccc tatcgccagc accatcccga
     4201 acatagtcaa cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac gtcccgagaa
     4261 taaccaccgg tacggcaatc gtcgggatga ttgtggcacg gaaattctgc aaaaacagat
     4321 acatgaccag gaagacgagg atgatagcct caaccagtgt tttgaaaact tcctgaatag
     4381 aaatttcgat aaacggcgtg gtgtcgtaag gataaaccgt cttcagactt gccgggaaat
     4441 aggctgataa gcggttcagt tcctctttga ctgcccgcga ggtatccagg gcgtttgctc
     4501 cggtagccag tttgatggcg atcccggcag caggtttgcc gttatagcgc gccacggtgg
     4561 aataatcttc cgccccaagt tcgacgcggg cgacatcacg cagcagcact tgcgaaccat
     4621 cttgctgaac tttcaacagg attttgccaa attcttccgg cgtttgcaga cgcgtctgca
     4681 caatgatcga ggcgtttagt tgctggtctg ccgcctgtgg catgccaccc agttgaccac
     4741 cagaaatctg gttgttttgc accttaatct gggaaataac atcggaaggt accagattgt
     4801 atttattgag tttttgcggg tccagccaga tacgcatggc gtactcggaa ccaaagagct
     4861 gtacgctacc aacgcccgcg gtacggctta gcgggtcttt gatattagac gctacatagt
     4921 ccgcgatatc gtactggttg aggctgccgt tatcagaaat aaacgccgct accatcagga
     4981 tattactgct cgatttatcg acgctaatcc cctgctgctg cactgcttca ggtaatgaag
     5041 gcatagcgag ttgcagttta ttttgcacct gaacctgtgc gatatcagga gatgtcccag
     5101 tctcgaaggt cagagtgata gaggcattac ccgccgcatc actggttgaa gacatgtaca
     5161 tcaggccatc aagcccattc atattttgct caatcacctg agtgaccgag tcttctaccg
     5221 tttgcgcatc ggcacccgga taggtagcgc tgacggtaat ggtcggtggc gcaatctgcg
     5281 gatactgcgc aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc ataataatgg
     5341 caagtaccca ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa gtccccttaa
     5401 gcctgcaacg ttattgtttc gattcggtgc tggcgttttc ctggctggag gaaattgctc
     5461 gtgctttgat acccggacga atgcgttgca aaccggaaac gatcacccga tcgccagcct
     5521 gcaagccaga ggtgacaacc cactggtcgc cgatggcttt gctggcttca atttcgcgta
     5581 gcttcacgac atcgtcttta tccagaatga gcgccgttgc tttaccttgg gcgttgtggg
     5641 tgacgccttc ctgcggcacc agtaatacat tctggcggct accttcatcc actaatgccg
     5701 tgacatacat gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc cgtaacgtca
     5761 cggagcccgt ggtttcatca accgtcgggt cggagaattt cagcgtgccg gtctggctgt
     5821 aacgtttacc attttccaga ttgagctgta ctggcgtact gccctgaacc tgtttgattt
     5881 gcccactggc gacctcttct ttcatgcgta agaaatcttg caccgactgc gtgagatcga
     5941 cataaatcgg gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga
     6001 gtgcgccgac ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag gtgacattcg
     6061 cgtattgcag attgatcgtc gcctgttcaa cagccgcttt ggcgacggtg acattggctt
     6121 ctgcttcatt caactgggtg cgcgcggtgt cgtaatcctg acggctaacg tagttggtct
     6181 tcagcaacga tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta gagagcgctt
     6241 tcgccagcga gcctttggcg gagtttagct cggcctgtaa aggtgcagga tcaatctgat
     6301 acagcgaatc gccctggttc actttatcgc cttcgataaa gttgcgttta atgataatac
     6361 cgcccacctg gggacgtatc tcggcaactt cataaggaac ggttctaccg ggcaattcgc
     6421 tcaacacatt gaccgaccca ggggagagtg tgacgacacc gacctcaggc gtcatggcgg
     6481 cggtgttttc cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag aataacaacg
     6541 gtattaacag ctttcttctt ctgttcattt tagtccctga aaattcttga gaaataacgg
     6601 ttctttaaat tgcgcgcgag gagagatatg agggatttac agaccagcgg catagtgggg
     6661 ttgtcagcca gtaaggtggg atacaggcat agtgatcgac atggtgaggt caacgacaaa
     6721 aaaaatgttg ccgttctgcc aacagttcct gccagcattc gggcaacgaa gggttctact
     6781 ggtggataca cataccaggg gaataaagtc gttttagcct ctcctttagt aatcaccggg
     6841 ggtaatgaaa tcagcatctg catccctcgt catgccagcc atcaatttca gttgcttatg
     6901 tcctgactaa aaataagatg tgatacccag ggtgacgatg tcgctcatac gtcttagttt
     6961 cagcttgtac ataatgttga atttctgaat ctttaatgtt tgttgcgaaa tattgagcgt
     7021 cgacgtgata ttgctttcat ttttattttt cagaatcagt tgcataattt tgaactgatt
     7081 atgtgaaaaa cataattgtg aattcttatg gggcaagtgt ttaccataag taatctcaag
     7141 aagatttttg atactttctt tgcggctaac aaaataaata ggcgctttag cttttagttt
     7201 actgatgtga taaggtgcca aaacaataat tctctcggca tctaatttct ccagaaattt
     7261 aatcgcttct tcatcaagga tatgattgtg ataggtatca atgataacct tgaagggttt
     7321 attgcctact tttttgatct ctgacaggga attgattttt atcatttcgt gattatcttt
     7381 caactgccaa aagccctgta aaagaaaaga atctttcgtc atgagaaaaa tcataacttg
     7441 ctccttagcc gttatcgttt cgaacatgtc atccttttca ttatttacat ccttgtccga
     7501 atcgttgttc aatatagtaa acgcctataa gaactttgcc cttcgtgaac taatacgtct
     7561 attaacacta atcaatactt cagatttatc catcacgcac tccatatatt gatcctaagt
     7621 aagattcctg gttgttatca gcttgtaaat agtaaacaaa aagttaacgt ttgcggatta
     7681 ctgcccaaga ataag
//
LOCUS       DAZKIF010000178         7682 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZKIF010000178 DAZKIF010000000
VERSION     DAZKIF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.179"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..589)
                     /locus_tag="ACHOTW_004255"
     CDS             complement(<1..589)
                     /locus_tag="ACHOTW_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP9846251.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWIGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKV"
     gene            790..1044
                     /locus_tag="ACHOTW_004256"
     CDS             790..1044
                     /locus_tag="ACHOTW_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9846252.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(1094..3064)
                     /locus_tag="ACHOTW_004257"
     CDS             complement(1094..3064)
                     /locus_tag="ACHOTW_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9846253.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     NDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLTFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRKRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFVEEGRNFWAGVEYTF"
     gene            complement(3090..3944)
                     /gene="modD"
                     /locus_tag="ACHOTW_004258"
     CDS             complement(3090..3944)
                     /gene="modD"
                     /locus_tag="ACHOTW_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFP9846254.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            complement(3941..4753)
                     /locus_tag="ACHOTW_004259"
                     /pseudo
     CDS             complement(3941..4753)
                     /locus_tag="ACHOTW_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(4763..5521)
                     /locus_tag="ACHOTW_004260"
     CDS             complement(4763..5521)
                     /locus_tag="ACHOTW_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9846255.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            complement(5518..6498)
                     /locus_tag="ACHOTW_004261"
     CDS             complement(5518..6498)
                     /locus_tag="ACHOTW_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9846256.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFVLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(6498..7520)
                     /locus_tag="ACHOTW_004262"
     CDS             complement(6498..7520)
                     /locus_tag="ACHOTW_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFP9846257.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLSEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFAASPAANLRFYAARGPRGLETGLKGSLHTEAAELLG
                     LHNVAQIADRQGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWHGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAT"
BASE COUNT         1839 a         1943 c         2026 g         1874 t
ORIGIN      
        1 tcactttgcg ctcgctgatg gagattaact gggcgtcaaa gtgaaaaaca ccccattgcc
       61 ccatgttgac ttcaatctga gcgaagcgca taccttcgtg caacccggca ggttttgctg
      121 tttccagtaa tgcgcccatg ccgcctaacg acaaatcata caggcggaaa cgtaacgtac
      181 tgttatccgc cagtttggtc tggcaaaaat aaggcggatg gagtggggcg gagatgcgga
      241 aatatcggcg tcgttgtaca aaccataagg tgggaggcgg tacggtaata aatgccggaa
      301 gctgcaagta ttcactctgc tgtagttgtt caacagtaaa ctcgactttc gcaccctgag
      361 tttcggcggt aatggtaatg tgctgtgcct ttagcacggc gatgttgtct tcggcttgac
      421 tgccgaaatc cagcaccagt ttatccgggg ttattaccag tattttgctg atcagctgcc
      481 cgccaatcca actgagacgc aaaggaattg cggctttgtg caaatcgcgt aacacgccca
      541 ggacggctaa cggattttgt ttcaggaact gctcatggta atgactcacg cggaaaaact
      601 cctgactgac aaactgtctt tggtttatcg gtcacagtta acaaaactta atacaaatgc
      661 gtgaatattt tttacatgtt gttcttaaat cagccgacat acgcccagca tcgatcccgc
      721 ccttgcctat acttagagcg ttgacgtaag catttcttgc gtccgattca tcgaaacgag
      781 ggcctgaaca tgggaattat tgcctggatt atttttggcc tgatagccgg cattatcgcc
      841 aagctaatca tgccggggcg tgatggtggt ggatttttcc tgacctgtat tctcgggata
      901 gtcggtgcgg tggtcggcgg ctggctggcg accatgtttg gcattggcgg ctccatcagc
      961 ggttttaatt tgcacagctt cctggtggcg gtggtgggag ctattctcgt tctgggcgta
     1021 ttccgcctcc tgcgaagaga ataagatttt cataaggcgg atagcgatac agatgccgct
     1081 atccgctttc acatcagaac gtatactcga cacctgccca gaaattgcga ccttcttcaa
     1141 caaacccttc actgtaggca tattgggtat caaacaggtt attgaccgac gcattgacgc
     1201 tgaagccatg acctaaggtg taatcggctc gaatgtgggt caccgcaaaa ccggcggctt
     1261 tttgtgaacc gtcactgtta ctgtagctgg aggaacgcgc ctcttccgac agcgttacgc
     1321 ttaacggctc ccacggtttg agagtcatcc atgcggtcat tgtctgcgtt ggcagatcgg
     1381 ttatcttgcc gatgtcttta cgtttggcgt cagcgtggat cagggcgtag ctcaatccta
     1441 catccagtat attgctgatt ttccccttaa taccggcatc cagaccgctg taatccaccg
     1501 tgccgctgtt ctgattttga atggtatcgg catcgatatt gtgcgagagg atggcatcac
     1561 tcacccggtt atagtaaacg ctgacctcaa atccccagtc gtgcgtgaag gcaccattcc
     1621 aggttaaatc caccccgcga gcgcgttccg gcttgagctg cgggttaaca atcgctatct
     1681 ggttatacgc aggtttggat gtggtatagc gttctttcag cgtcggaaag cgtttgcggt
     1741 catagtacga aagcgccagc gtgtcttcat tgacaaagtg gtatttcccc atcacctgcc
     1801 agttaaaagc tgactgattg ttgtcgtcat aatgggtgat gctgccatct ttctcgtgtt
     1861 ttttcgcttc tacgctatcg cgccagtcat agctgattcc agccacaaca tcgacattat
     1921 cggcagcagc ccattgatat tcactggcga gcgaccaggt acgatcttca tagcgatcgt
     1981 aagcggcgtg cggcgcacct ttttcccggt gtacgtcatc tttccagtta acggcaaacg
     2041 tcagcagatc gttttcacgc acatcggctg ccagttgtaa tccggcaccg tcgctgtaat
     2101 cggaataatg gctgtagctg ccttttttat ttttcaaatc agccagcgag ttgtacatca
     2161 tcagcgtatt ttcaaaggtg tcgcgataca gccgactttt cagggtaaaa cgatcgttta
     2221 gttgggtcgt tccctgataa taaaaacttt ctttgtcata ctctggccac tgccagtagc
     2281 gtgatttttg accactattt ccgctgtatg gcgggttatc tttttcacca tcctgcttaa
     2341 tgtaagtcag tgtgtattca tcgttttcac gtggtgtaaa tccgagcttc acaatgccgc
     2401 gtttatcatt agccgatgaa ttaatcatct tgccgtgttt gcctgcaata tcattattta
     2461 caccatgcgg caggccgaga aaatcctgct ttagctggct accgctgact tgcaaatacc
     2521 ccagttcgct gctggcggca aatgaagcat gcatatcata ggcattgtcc tggctacggc
     2581 tccatccctg gcgatatccc agacttgctt ccagaggttt tgttggcttc tgggtggtga
     2641 tattaatggc tccgcccatc tgattaggtc cctgaagcag cgacgaatac cctttggaaa
     2701 cttcgactgc ccccagattg ttggtcagaa tccgcgccag atcgaggttg ccgtcatagg
     2761 gaacataaat gggcacaccg tcgaaataga ccggcacctg ccgactatca aagccacgaa
     2821 cttttacctg ttcttcgttg cggctgcctg acttttgcag caccacgccg gggacgacac
     2881 ttaatgcctg agcgacattc tgtttatcca gggccttcat ggtgggttga tcgagaacgg
     2941 tggtcgtcgt cgatgatacc ggactggacc atacggtcag cgagtcgctc tcctgagccg
     3001 ctacggcctg ctgggtaaag gcaagtgata atgctgtgca gagataatgt tttttgagtc
     3061 tcatagaata aattccgttt aacaaagaat taaatagagg ctgcgggttg cagactgact
     3121 ttaatgtcag ccggtgccgc gtaataaggc gcggaggtaa taaagaggcg aatgccgcag
     3181 tcgaggtaat ttttgagtgt cgtcagatta attccgccgg tcagagccag cgtgcagtgg
     3241 ggagccagcg acggcgctat ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg
     3301 agctgaagta cgtctggttg cgcgcgtaac gcggcgattg cctctttcgg cgtgtcggct
     3361 tcaacaacaa ttttcttctc gggtgcgtgg cgacgtaact gattgattgc gcctgaccag
     3421 tcctgattgt catgaagaaa atggcggtgg ttggcaaaca ataatatggt ttccgcacat
     3481 ccggcacgat gaatcagtcc tccggcagcc agaattgcct gcgaggccag taagcgggtg
     3541 cccggaattg cttttcgggt gcaggcgata ttgccatcag gataacgttc acgaagtaac
     3601 gccagcattt gatcgagata atcagaaaca ccgcaactcc actccagcac attctggatt
     3661 gccttccatc cctgatgaag tgctgcggca ttaccctgcg cacggattag ccgctgaccc
     3721 gcgttcgctt gtgaaccgtc gctgaccgcg tcatcaatag ttaatcccag cgtagttaac
     3781 atcttacacg cgacagaaac gccgctgacg cagccgccct gacggagaaa aaactctata
     3841 tagccatgct ggtgtccaat atttaatgct ctcgtggtca ggtcaccgcc ctggatatct
     3901 tccagaagta acgcatcgat ttgtgcctga gaaaggaaaa tcatcgcagt gcctcctgcg
     3961 gtacgctatc ccaccagatg agcgcccagt cgcggctggc aggggcgagg gcacgagcat
     4021 cttgttgttg ataccagcga tataaccgct cacgctcatc gtcgttaagc gcgccaagac
     4081 tccagctgac gttctgctca aaacgttcct aggtgctgtt atcctgttgg caattttgtc
     4141 cacggataaa atcaacgtgg gcataaattc ccatctgata caggacgttg agcgcaaaga
     4201 tatagttggg taattcgatc acttcccgac cagccgcccg ctgaatggct ggagagacga
     4261 acgaggtgct gaccagatga gtcgtataga cgcgaagccg tgcctgatta ttcagtttgc
     4321 tcattgcctg gcgcatatca gccaccagag tggagcggga agccacggca atatcgcagc
     4381 gtggcaggtc actccagtcc tcttcccagg cgcgctggat caagtggaca ttgtctgcct
     4441 tcagcgcggc ggcgcgacgg gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat
     4501 aaatcgtcgt gagtttgtca gccagcgcca gcgatacggt acccggtccg cagcccatat
     4561 cgaataaggt ctgagcgcct tgtaggtcaa ttttagcgat cagttgttgc aggtagctgt
     4621 ccgtaggcga ggcgcaattt tccgccattt tttccgcccg ttgatcccag tgatccggcg
     4681 ttttttctgt gcggtgggcc agcttgagtt gctgcagata aaggtctgca aaatcaatat
     4741 catcaatgag catgattttt ccttagtggc taattgcaga gaggtgatga tgaatctggt
     4801 cggcagaaac ccgatacagg gcagccagtt tgtttgttgt taactgttcg gtcggtaagc
     4861 cttgtgttac tcgtccatct ggctctacct ggataatgct gtcggcaatg gcgttggcat
     4921 ggagcggatg atgagtcgac attaacattg tcatgccgtt atttttgagc tgtgccagcg
     4981 tgtccagtag ttggatctga tgaccgaaat cgaggctgga ggcaggctca tccagtaaca
     5041 acaagcgcgg ttgctggacg agtgcacggg cgattaaagc cagctgacgt tcaccgccgc
     5101 taagcgtatt ccagcgtcgt gccgccagat gcaaaatgcc cagcttttcc agttgctccg
     5161 tggctttcag gcgttcttgc ttaccgggaa cggaaaaagc gcctatggtg ggggccaggc
     5221 ccatcaacac catatccagc acactaaaag caaaaatacc gtcgtgcgcc tgcggcaccc
     5281 aggcaatagc ctgcgcgcgc tggcgatggc tgagtgtctg cacagggagg ctatccagta
     5341 agacctcgcc ttttaataac ggtataacgc caagaatgga gcgcatgagt gtggttttgc
     5401 cgcaaccatt ggctcctaac agacaacaaa ttgttccttg cgacagggtg aaggacacgt
     5461 cgcgtagcac cggatggcgg gagtaaccca gagacgcatg acggacggca agcaaagtca
     5521 ctggcgacct ccgcgtaaca acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag
     5581 aatgccaagt gggatttctg ttgttccaat gctgcgtgcc agcgtatcgg taagcaggag
     5641 cagaatcgcg ccagtacaca ttgccatggg caacagctgc tgatggttat gaccggtcaa
     5701 caagcgggca atgtgcggaa cgaccaggcc gacccaacca attatgcccg ctattgctac
     5761 cgtactggcg gtgattagtg tggcgcagac aatcaataca aagcgcagac gcgtcacgtt
     5821 cagtcccagc gaacgggctt cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag
     5881 aaaaaacagg ggcaaggagc ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa
     5941 ggtcacggtg gacagtcccc ccagcaacca gaaggtaatt gacggtaact gggtgtaagg
     6001 atcggccagc gttttaatta gcgatatccc cgctccgcac aacgttccta acgcgatgcc
     6061 taccagcacc agggttaaga tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac
     6121 gcccgcgacc accatcagcc cgccacaaaa tgcgtacagc tggatgtaaa caatggataa
     6181 tccccacaga attgccgtgc aggcaccaag tccggcaccg gcggagacgc ctaaaatatc
     6241 cggtgaaact aagggattac gcaacattcc ctgataggtg gttccggctc ctgccagcgc
     6301 cgcgcccagt aacagcgcag caagaatacg cggcacgcgg atttgccaga agacaatttg
     6361 ttcctgcaca ggaacatttt cttgacgaag aattagcgcc agcagttgct gcatatcgag
     6421 atgataagcc ccacttacgg cggcaaaagt gatactgata gctgccagca gcaaaataca
     6481 tccgtttacg attctcatta ggtcgccacc aatttttgat agtcggcgtc actgagtgaa
     6541 caatgccaga acagttgggc gtaatgctgc atgtcacttt taaactggcg attgatggcg
     6601 ggatccagcc aggcgtgaag tctgcgtaat cccagaagac ggttgattcc cggtggggca
     6661 tccagccagc caaagggcaa gccacttaaa aataagatgc gttgttctgc aacggctttc
     6721 acgccatgcc agagaggatc acgacgaata aaatctgcag taacggcctc ctgaaccaga
     6781 ataatatccg gctgccagcg caggagattt tccatggaaa cctgtgtcag gccttggcga
     6841 tcggctattt gcgcgacgtt gtgcaaacct aataattccg ccgcctcggt atgcaacgaa
     6901 ccctttaagc ccgtctccag ccctcgaggg ccgcgcgcag cataaaagcg gaggttagcg
     6961 gcgggtgagg cggcgaatgc ctgagcttca ccaacgaagc ggctggcgag attggcttgt
     7021 tctgcggcgc gatgctcttc gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat
     7081 tgcgccagtt tcccgttaag caataaccag ggtatttgtg tctgttcgct aacctgccgt
     7141 gcctgggaga tccaggtttc atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac
     7201 gccataagcc cttccagcga gagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt
     7261 aactggcgaa tgtgctctga caatggaatt aatgcctgac gggcaaaatc aaaagaggaa
     7321 aaaccgacca ttttttctgg cgcgacagcc agcaatagca aatcggcggg cgcacctgcg
     7381 ctgacgatgc gtgtaatgtt ttgggcttcg ggaagcgtca gtgaggccca gaatttgact
     7441 gattgtgcaa aagaaggcag atgatgcagg aacaacgtcg acgcaagaat ctgtgtaaac
     7501 ctccgtctgg ttagtgacat aagttatatc ctgataatta aaaccgctat atatttacat
     7561 atatatcgaa aaaaatcatg tttgatgtca aaatttgtct aaagagacag aatgatttgc
     7621 ttgttgctta aataacactt tgttccacaa gaaaattgtc agggagaatg gcggagtggc
     7681 ga
//
LOCUS       DAZKIF010000179         7650 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZKIF010000179 DAZKIF010000000
VERSION     DAZKIF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.180"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..747
                     /locus_tag="ACHOTW_004263"
                     /pseudo
     CDS             1..747
                     /locus_tag="ACHOTW_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1038..2279
                     /gene="agp"
                     /locus_tag="ACHOTW_004264"
     CDS             1038..2279
                     /gene="agp"
                     /locus_tag="ACHOTW_004264"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP9846258.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(2317..2544)
                     /gene="yccJ"
                     /locus_tag="ACHOTW_004265"
     CDS             complement(2317..2544)
                     /gene="yccJ"
                     /locus_tag="ACHOTW_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP9846259.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(2565..3143)
                     /gene="wrbA"
                     /locus_tag="ACHOTW_004266"
     CDS             complement(2565..3143)
                     /gene="wrbA"
                     /locus_tag="ACHOTW_004266"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9846260.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMSPQLFEK
                     AGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            complement(3140..4450)
                     /locus_tag="ACHOTW_004267"
     CDS             complement(3140..4450)
                     /locus_tag="ACHOTW_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9846261.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYADRFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEIGSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSRKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLSRQHQITVQLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSSGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNVLNARAPDVPQSDQEDEIKLYFSK"
     gene            complement(4503..4787)
                     /locus_tag="ACHOTW_004268"
     CDS             complement(4503..4787)
                     /locus_tag="ACHOTW_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9846262.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            complement(4873..5172)
                     /locus_tag="ACHOTW_004269"
     CDS             complement(4873..5172)
                     /locus_tag="ACHOTW_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846263.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAVRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            complement(5244..5528)
                     /locus_tag="ACHOTW_004270"
     CDS             complement(5244..5528)
                     /locus_tag="ACHOTW_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9846264.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLFNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            complement(5521..6000)
                     /locus_tag="ACHOTW_004271"
     CDS             complement(5521..6000)
                     /locus_tag="ACHOTW_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9846265.1"
                     /translation="MGIELVKYGCNTFICPDNSMEPGNVPLYIGLPRIDPASQTAKLS
                     FQEWLSEQKEKIDVDCGCVSIETLTHWMKSAYEAGNSPVTPDSWISCSDRMPEKGQNV
                     LISVNFDSSLVEPLICSARYTGSTFRRGDATIKPGNGIEQATHWMPLPEPPQEVNRG"
     gene            complement(6013..6144)
                     /locus_tag="ACHOTW_004272"
                     /pseudo
     CDS             complement(6013..6144)
                     /locus_tag="ACHOTW_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6148..6435)
                     /locus_tag="ACHOTW_004273"
     CDS             complement(6148..6435)
                     /locus_tag="ACHOTW_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846266.1"
                     /translation="MATLQELIDLTPEQEKAWNRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTASVYMPSIDAPGLTSWADDWHGITLKDGVEVDKD"
     gene            complement(6437..6655)
                     /locus_tag="ACHOTW_004274"
     CDS             complement(6437..6655)
                     /locus_tag="ACHOTW_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFP9846267.1"
                     /translation="MPTLFRKEYPRKSRATEFLFLILFIVLMTPISPLIFVWAIGKII
                     ELVIELYNDVVWASFNTLHNKINPYKEN"
     gene            complement(6657..6872)
                     /locus_tag="ACHOTW_004275"
     CDS             complement(6657..6872)
                     /locus_tag="ACHOTW_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846268.1"
                     /translation="MAIAASYTMHLYCDCLQCTDGKYKSPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKILRSNKGE"
     gene            complement(6874..7062)
                     /locus_tag="ACHOTW_004276"
     CDS             complement(6874..7062)
                     /locus_tag="ACHOTW_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846269.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAEQNRWKNHLRTKGE"
     gene            complement(7214..>7650)
                     /locus_tag="ACHOTW_004277"
     CDS             complement(7214..>7650)
                     /locus_tag="ACHOTW_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846270.1"
                     /translation="VELEAAENNLIDSECHVAELEEALRDKQALLEASEKRNAKLQSE
                     NAYIRNRYKELDLLIGKNILVMQAAIIEWQATGDAKSGLAWIYNTLFGPGELPDESEK
                     DAQAYFNRKYAPIDEKLMALHKWFWEQSEAERAAGIRIKRGE"
BASE COUNT         1862 a         1833 c         1755 g         2200 t
ORIGIN      
        1 taaatacgcc gggaatatct gatttagatt ctggtattga tttgtctggt atatctttta
       61 ttcagagtga aattaataaa acttatggat taaaatacgc tccagtagat ggcgatggct
      121 attgtctgtt aagggccata ctggttttaa aagaacatga gtattcatgg gcgctgggca
      181 gccataagac gcaaaagcaa gtatacgaag agtttattaa aattgttgat aaacaaacga
      241 tcgaggctct tgttgatacg gcattctata atctcaggga agatgtaaag acgttatttg
      301 gcgttgatct acaatctgac aacaaaattc aggggcaggg tagtcttatg tcatggaact
      361 ttctgttttt taaaaaaaga atttattgat agttgcttga atgacaaaaa atgcatactg
      421 catttacccg agtttatatt taatgataac aaggcgcgcc ttgttttaga caccgaccca
      481 gagaagagga ttaatgaggt gaaaaatttc ctcactgctc tttcagatag catttgctca
      541 ttatttattg ttaatagtaa tgtggcatca atctccttag ggaatgaatc cttttcaaca
      601 gatgaagatc ttgagtatgg ttatttaata aacactggca atcattatga cgtttacctc
      661 cctcctgaac tttttgctca ggcttacgag ttaaacaata aggaaaggaa tgcgcaaatt
      721 ggttttttaa ctcgttatgc aatttaatga cagaggcata tgctaaaaac cattgttatt
      781 agtctcacac ttttttattg gtaaatattg cctctgtatt ggtaacgccg ccgatattct
      841 gtttagccac aggtgcaatc atcagcggcg tacgcgaggc aggggctaat caggcatagt
      901 ttgcgtcaaa ccttgcctgt ttttaaagat gaatataaaa acagccgttc aacaagccat
      961 tttgcgaacc tgttcccgga aaaaagtcat atttctgtca cactctttag tgattgataa
     1021 caaaagaggt gccaggaatg aacaaaacgc taatcgccgc agctgtggca ggggtagttt
     1081 tactcgcttc aaacgctcag gcacaaaccg taccggaagg ctatcagcta cagcaagtgc
     1141 tcatgatgag ccgccataac ttacgtgcgc cgctggcgaa caatggcagt gtgctggagc
     1201 agtcgacgcc gaataaatgg cccgaatggg acgtccccgg tgggcaactc accaccaaag
     1261 gtggcgtact cgaagtgtat atgggccatt acatgcgtga atggctggca gagcagggga
     1321 tggtaaaatc gggggaatgc ccgccgccgg acaccgttta tgcctatgcc aatagtctgc
     1381 aacgtaccgt tgcgaccgca cagttcttta ttaccggcgc attcccgggg tgtgatattc
     1441 ctgtgcatca ccaggaaaaa atgggcacta tggacccaac ctttaacccg gtgatcaccg
     1501 atgattccgc cgcattcagt gaacaggcgg tggcggcaat ggagaaagag ctcagcaaac
     1561 tccagcttac cgacagctac cagctactgg aaaaaatcgt taactataaa gattcccctg
     1621 cctgtaaaga gaaacaacag tgttcgctgg tggatggcaa aaataccttt agcgccaagt
     1681 atcaacaaga accaggtgtt tccgggccgc tgaaagtcgg caactcgctg gtagatgcgt
     1741 ttactttgca atactacgaa ggttttccga tggatcaggt ggcctgggga gaaatcaaat
     1801 ctgaccagca gtggaaggtg ttgtcgaagc tgaaaaacgg ttaccaggac agcctgttta
     1861 cctcaccgga agtggcgcgc aatgttgcga aaccgctggt cagttatatc gacaaagccc
     1921 tggtcaccga tcgcaccagt gctccaaaaa tcacggtgtt agttgggcat gactccaaca
     1981 ttgcctctct gttaacggcg ctggatttca aaccgtatca gttgcatgac cagaacgaac
     2041 gcacgccgat tggcggcaaa atcgttttcc agcgttggcg tgacagcaaa gccaatcgcg
     2101 atttgatgaa aattgaatat gtgtaccaga gtgcggaaca gttacgtaat gccgatgcgt
     2161 taaccctgca ggcacctgcg cagcgcgtga cgctggaatt aagcggttgc ccgatagacg
     2221 ctgatggttt ctgcccgatg gataagtttg atagcgtgtt gaatgaagcg gtgaaataac
     2281 agaaaactcc cccgttcgct acgggggagt cgctggttaa acgtttttac gttcgatggt
     2341 ctgttcgccc caaaaaagcg aatctttgtc ggtcttagca aaggctttga ctaacacttc
     2401 atcgctacct tcttcccaaa tcttttccgc cattttttcg tcgtacccgg cgacttcgaa
     2461 aatggcctcg gctatttccg gcgacgtatt gcgcagagat gcccattcac cgacgtgatg
     2521 agctttcgct tcttgagttg gcatgcgtat cctcctgttg aagattagcc attaagttta
     2581 actgccagac ctgcgacata ttccccttga taacgagcaa tagacagttc ttcctggctg
     2641 ggctggcgtg aaccgtcacc gcctgcgatg gtggttgcgc cgtacggcgt accgccgcga
     2701 acctgtgaaa cgtcaaataa ttcctgcgct gcgtagccaa tggggacaat caccatgccg
     2761 tgatgcgcaa gggtcgtcca ggtggatgtg atggtttgtt cctgaccgcc gccagtaccg
     2821 gtggaactaa agacgctcgc cagttttccg tatagtgcgc cggaagccca caggccgccc
     2881 gtctggtcga ggaaggtacg catttgaccg gacatgttgc caaagcgggt aggtgtacca
     2941 aaaataatgg cgtcgtaatt ggccagttct tgcggggttg caaccggtgc agtttgcgtt
     3001 ttaccgcctg ctttttcaaa taattgcggc gacatggttt ccggtacacg cttaacgaca
     3061 acttcagcgc catccacttt gcttgcaccc tcagcgactg cgcgtgccat cgtttcaaca
     3121 tgtctaagga tatggtttat cattttgaaa aatataattt tatttcatcc tcctggtcac
     3181 tttggggcac gtctggggca cgggcattaa ggacattatt caacatggca acttgagtca
     3241 cgctgcactc aggcatccat gcaccataaa cattgtagac catgctggcg ctggagtgcc
     3301 ccatctgtga tgcaataaat gtcgggtttg ctccggaaga taaagcccag cacgcatagg
     3361 tatggcgtga ctgatacgct ttacgggatc ggatacccgc tctttttatt gctgaatccc
     3421 atgtcgctcc gatggagctt accgcgtagt taatacccgc cttgtgattc ttgcgaacga
     3481 tttgcggaca gaaaacaaaa gtgcactcgt gcaaaattgt tcttccgtac tcgcgtaatt
     3541 gaacagtgat ctgatgctgc ctgctaagac gagtaagcat cgcctggttt ttaagtgctt
     3601 caattgctgg tgccagaaga tgtataaccc ggttagtgcc tgcgtcggtc tttggtagcg
     3661 taaaatcacc tatttttgta aaatttcgtc gcactgttat cgtgccagct ttcaggtcga
     3721 tatcctccca tgcaagtgcg gcaatttcac cgtgtcgcat ccctgtaaaa acagccactg
     3781 tccagaggtt tttggtctgt tgatgatggc aggcatcaat gagacggcta aactcatctc
     3841 tggtcagtgg atccggcact ggttttgatt tcctcagcgg tgttattgaa ttaaacgggt
     3901 ttttctccag atacccgttt tcggcggcaa agctgaacat tccggctgtt gttgtcatgt
     3961 aatagttcac tgtgggtacg gttcttcctt ttcgggacgt gctggttttc ctgcttccct
     4021 tttccccggt cagtaaatct ttcctgataa acagcagatc ttcttttgta atcgatgacg
     4081 ccagcctgcc aggaccaagc ctcggtagca tatttttcat cactgattga taacgattta
     4141 aggcgttact accgatttcc attgctttca gagtaagcca tttctgtgcc agttcaccga
     4201 cggtgatatc tttttttacc aggccaaata gcttcaggtt aggtgagtca gggaatcgat
     4261 cggcataatc aaacgttcct gttctgattg caaagcagac cgaagccctt aactcaccag
     4321 cgatctttct gtttttcggt gtatcgggca cgcgaagatt ttcgcgcact cgtttacccc
     4381 tgtacttaaa cgttattcgg agttttcctc cgtgattttc aacgccggct ggataggctg
     4441 aattcgccat tgttcctcct gcgtccaaga gcacgctcag gttatactct tatggacaag
     4501 aattaaacat cctgagatgg taagggttgg tttttgatcc aagtgttgat ggtaggaaga
     4561 tgatagagac attcgctgtt tttctttggc gttccgtcgg gggcgatttg tttatattct
     4621 ctgccgttca tccacgcact gtccctggcg cgtaatattg ttccttttct caggcctgtt
     4681 acagccatta aaagctccag tgttacccat tcattcgcat ttatctgtac aacaggtgtg
     4741 atgactggct gaatgccatg gttatgttgg tttactaact cgcgcataag ttgcctctgc
     4801 atgtgtaaga aaaaaccgcc atcaggcggc ttggtgttct ttcagttctt caattcgaat
     4861 attggttacg tcttattcga tgcgcactcc tggtatttcg ccttttgata ttgctaagtc
     4921 ataaatttgc gcggcactat acccatctcg catccatgaa tctaaggcgc gaacagcctc
     4981 gctacgcttt ttatcttctc tctcattttt gatatcaacg aggacatcaa cgcaattaag
     5041 gcaaatgtgg attttgtctt tacattcgat catggcggct ttgccatgat ttccgccaca
     5101 cagtgagcat aaatcttcag ggtctggctg gtatttctgt aacgttagag ggttgaatgt
     5161 tgaacaggcc ataatcatct ccataaaaca aaactcgccg tagcgagttc agataaaaga
     5221 aatccccgcg agtgcgagga ttgttattca ttgccgatat tcacctttat cgcgaacacc
     5281 tttaccggtt tatcaccgaa gtgcggatgt gtgattgtct tgatttcata tccgtcatac
     5341 ggaacatcaa ttcttcggct ggaatcgtcg cgcttcggat atccctttgt gataatcagg
     5401 cggtcatact cgcggaacat aattcgctta ttccagtagt cattaaacag gcgatactct
     5461 tccgttttct ctccgcgaat catggcatcg aagtattcac ctttaacggc aagttgcagg
     5521 ttagccacgg ttaacctcct gcggcggttc tggtagcggc atccagtgag ttgcttgctc
     5581 aataccatta cccggcttaa tcgttgcatc tccgcgccga aaggtgcttc cggtatagcg
     5641 tgcggagcat attagcggtt caaccagaga gctatcgaaa ttcaccgaaa taagcacgtt
     5701 ctggcccttt tcaggcattc gatcactaca gcttatccaa ctatccggag ttaccggaga
     5761 gttaccagcc tcataagcgg atttcatcca gtgcgtaagc gtttcgatgc ttacacatcc
     5821 gcaatcaacg tctatttttt ctttttgttc tgacaaccat tcctggaatg acagcttggc
     5881 agtctggctt gctggatcaa ttcgtggcag gccgatatat agtggtacat ttcccggctc
     5941 catcgaattg tcgggacaaa taaacgtgtt acaaccatat ttaacgagct caattcccac
     6001 tgtgtcgata gtggcgaatg gttcagtggt caatgcagtc aacgcaattt cataagcacg
     6061 gcgctcaata ttatctcgca cgtccaggct gccgattcgc tctttgattt ctttaatcat
     6121 ttctttgtcg gtgaacgttg tcatgtgtta gtccttatcc acttcaacgc catctttcag
     6181 cgtgatgccg tgccaatcat cagcccaact ggttaaccca ggcgcatcaa tgctaggcat
     6241 atagacgctt gcagtgtggt agcccttatc gttatcaatg ctggcaacgt gctcgccgtt
     6301 gtatgcgctc agcgtgtcta ggacactata aaactttcct ccggctgccc tgaaatcctt
     6361 tacagccttc acaagacgat tccacgcttt ttcctgttct ggcgtcaggt cgattaattc
     6421 ctgcaaagtt gccatttcag ttttccttat atgggttaat tttattgtgc agtgtgttga
     6481 acgacgccca taccacgtcg ttatacaact caataactag ctcaattatt ttcccgattg
     6541 cccagacaaa aattagcggg gatatcggtg tcatcaacac gataaacaga atgagaaaca
     6601 aaaattctgt cgctctactt tttcgcggat attcttttct gaataatgta ggcacatcac
     6661 tctcctttgt tgctcctcaa aattttatgc cctggcgcaa aagcacgcgt tttgtctttg
     6721 cttattcgcc agccatcctt gcgcgcctct tttgcacagc cagcccatga cgtaccgata
     6781 tactcaccga agtctggcga cttatatttg ccatctgtac actggaggca atcacaatag
     6841 agatgcatgg tgtaacttgc agcaatagcc atatcactct cctttagtgc gcaagtggtt
     6901 tttccagcgg ttttgctccg cgctgggctt tttgcaaaaa ccacaatcca tcatcccgta
     6961 atatttcatc aaccccatcc gtcggttgct gagtctcacc cactgccaga cgccaggagc
     7021 gtttctacga actaacagaa tctttgcttt acggtttttc atcgttttgc tctcctgcat
     7081 ctctttgctg ctcgtcgtgc cgctgcaata ccggtatggc ggcgctttgg tgtcgggatg
     7141 atgttctttg caattagcgc agaagcccaa aaacgagtcg gatacggtaa caagccgata
     7201 catgccacac gcattactca cctcttttga tgcgaatgcc agcggcgcgc tcggcttcac
     7261 tttgttccca aaaccacttg tgaagcgcca taagcttttc gtcaatcggt gcatatttgc
     7321 gattaaagta ggcctgagca tctttctcag attcgtccgg taattcgcca gggccaaaca
     7381 gtgtgttata aatccatgct agtccgctct tagcgtcgcc agttgcctgc cattcgataa
     7441 tggcagcctg catgaccaga atgtttttcc cgattaatag gtccagttct ttgtaccggt
     7501 tgcggatgta tgcattctcg ctttgtaatt ttgcgttgcg cttttctgag gcttcaagta
     7561 acgcctgctt atcgcgtaga gcttcttcca gttcagcaac atggcattca ctatcaataa
     7621 ggttgttctc tgctgcttca agctcaacac
//
LOCUS       DAZKIF010000180         7619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZKIF010000180 DAZKIF010000000
VERSION     DAZKIF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.181"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(139..282)
                     /gene="ypaB"
                     /locus_tag="ACHOTW_004278"
     CDS             complement(139..282)
                     /gene="ypaB"
                     /locus_tag="ACHOTW_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP9846271.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
     gene            410..2695
                     /gene="nrdA"
                     /locus_tag="ACHOTW_004279"
     CDS             410..2695
                     /gene="nrdA"
                     /locus_tag="ACHOTW_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9846272.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2922..4052
                     /gene="nrdB"
                     /locus_tag="ACHOTW_004280"
     CDS             2922..4052
                     /gene="nrdB"
                     /locus_tag="ACHOTW_004280"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9846273.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4052..4306
                     /gene="yfaE"
                     /locus_tag="ACHOTW_004281"
     CDS             4052..4306
                     /gene="yfaE"
                     /locus_tag="ACHOTW_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP9846274.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4360..5010)
                     /gene="inaA"
                     /locus_tag="ACHOTW_004282"
     CDS             complement(4360..5010)
                     /gene="inaA"
                     /locus_tag="ACHOTW_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP9846275.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5091..6281)
                     /locus_tag="ACHOTW_004283"
     CDS             complement(5091..6281)
                     /locus_tag="ACHOTW_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9846276.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            6433..7311
                     /locus_tag="ACHOTW_004284"
     CDS             6433..7311
                     /locus_tag="ACHOTW_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9846277.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
BASE COUNT         2028 a         1983 c         1810 g         1798 t
ORIGIN      
        1 tcgcttacat tcgcttatat attgaccaca actgatacat cagattatgt gatgactcgt
       61 gcttagatca atttttgcaa tcattagcaa aaagattaat aaaccatcta tatcaattta
      121 tccaacctat tctgccgttc aagaaatcgc cgaacagtta tttataacaa atttttctct
      181 tcccattgac tttcccggac accttgtctg acctaaggtg cgcgaaagcc actttttcct
      241 tcctgagtta tccacaaagt tatgcacttg caagagggcc attttcacac tatcttgcag
      301 tgaatcccaa acataccccc tatatatagt gttctaagca gcttcccgta ctacaggtag
      361 tctgcacgaa actattgcgg aaagaattcc aaaaacaggt acgacataca tgaatcagaa
      421 tctgctggtg acaaagcgcg acggtagcac agagcgcatc aatctcgaca aaatccatcg
      481 cgttctggat tgggcggcag aaggactgca taacgtttcg atttcccagg tcgagctgcg
      541 ctcccacatt cagttttatg acggtatcaa gacctctgac atccacgaaa ccattatcaa
      601 ggctgccgca gacctgatct cccgtgatgc gccggattat cagtatctcg ccgcgcgcct
      661 ggcgatcttc cacctgcgta aaaaagccta cggccagttt gagccgcctg cgctgtacga
      721 ccacgtggtg aaaatggtcg agatgggcaa atacgataat catctgctgg aagactacac
      781 ggaagaagag ttcaagcaga tggacacgtt tatcgatcac gaccgtgata tgaccttctc
      841 ttatgctgcc gttaagcaac tggaaggcaa atatctggta cagaaccgtg tgaccggcga
      901 aatctacgaa agcgcccagt tcctttatat tctggttgct gcgtgcctgt tctcgaacta
      961 cccgcgtgaa acgcgcctgc aatatgtgaa gcgtttttac gacgcggttt ccacatttaa
     1021 aatttcgctg ccgacgccaa tcatgtccgg cgtgcgtacc ccgactcgtc agttcagctc
     1081 ctgcgtactg atcgagtgcg gcgacagcct ggattccatc aacgccacct ccagcgcgat
     1141 tgtgaaatat gtttcccagc gtgccgggat cggcatcaac gccgggcgta ttcgtgcgct
     1201 gggtagcccg attcgcggtg gcgaagcgtt ccataccggc tgcattccgt tctacaaaca
     1261 tttccagact gcggtgaaat cctgctctca gggcggcgtg cgcggcggtg cggcaacgct
     1321 gttctacccg atgtggcatc tggaagtgga aagcctgctg gtgttgaaaa acaaccgtgg
     1381 tgtggaaggc aaccgcgtgc gtcatatgga ctacggggta caaatcaaca aactgatgta
     1441 tacccgcctg ctgaaaggtg aagatatcac cctgttcagc ccgtccgacg taccggggct
     1501 gtacgacgca ttcttcgccg atcaggaaga gtttgaacgc ctgtatacca aatatgagaa
     1561 agacgacagc atccgcaagc agcgtgtgaa agccgttgag ctgttctcgc tgatgatgca
     1621 ggaacgtgcg tctaccggtc gtatctatat tcagaacgtt gaccactgca atacccatag
     1681 cccgtttgat ccggccatcg cgccagtgcg tcagtctaac ctgtgcctgg agatagccct
     1741 gccgaccaaa ccgctgaacg acgtcaacga cgagaacggt gaaatcgcgc tgtgtacgct
     1801 gtctgctttc aacctgggcg caattaatag cctggatgaa ctggaagagc tggcaattct
     1861 ggcggttcgt gcacttgacg cgctgctgga ttatcaggat tacccgatcc cggccgccaa
     1921 acgtggagcg atgggtcgtc gtacgctggg tattggtgtg atcaacttcg cttactacct
     1981 ggcgaagcat ggtaaacgct actccgacgg cagcgccaac aacctgacgc ataaaacctt
     2041 cgaagcgatt cagtattacc tgctgaaagc gtctaatgag ctggcgaaag agcaaggcgc
     2101 gtgcccgtgg tttaacgaaa ccacttacgc gaaagggatc ctgccgatcg atacctataa
     2161 gaaagatctg gatgccatcg ctaatgaacc gctgcattac gactgggaag ctctgcgtga
     2221 gtcaatcaaa acacacggcc tgcgtaactc cacgctttct gctctgatgc cgtccgagac
     2281 ttcttcgcag atctctaacg ccactaacgg tattgaaccg ccgcgcggtt acgtcagcat
     2341 caaagcgtcg aaagacggta ttctgcgcca ggtggtgccg gactacgagc atctgcacga
     2401 cgcctatgag ctgctgtggg aaatgccggg taacgatggc tacctgcaac tggtgggtat
     2461 catgcagaaa tttatcgatc agtcgatctc tgctaacacc aactacgatc cgtcacgctt
     2521 cccgtcagga aaagtgccga tgcagcagtt gctgaaagac ctgctcaccg cctacaaatt
     2581 cggggtcaaa acgctgtatt atcagaacac ccgtgacggc gctgaagacg cgcaggacga
     2641 tctggtgccg tcaatccagg acgatggctg cgaaagcggc gcatgtaaga tctgatattg
     2701 agatgccgga tgcggcgtaa acgccttatc cggcctacgg ctcggtttgt aggcctgata
     2761 agacgcgcca gcgtcgcatc aggcacagga tgcggcgtga acgcctaatc cggcctacgg
     2821 ctcggatttg taggcctgat aagacgcgcc agcgtcgcat caggcacagg atgcggcgta
     2881 aaatgcctta tccggcatta aactcccaac aggacacact catggcatat accacctttt
     2941 cacagacgaa aaatgatcag ctcaaagaac cgatgttctt tggtcagccg gtcaacgtgg
     3001 cccgttacga tcagcaaaaa tatgacatct tcgaaaagct gatcgaaaag cagctctctt
     3061 tcttctggcg tccggaagaa gttgacgttt ctcgtgaccg tatcgattac caggcgctgc
     3121 ctgaacacga aaaacacatc tttatcagca acctgaaata tcagacgctg ctggactcca
     3181 ttcagggtcg tagcccgaac gtggcactgc tgccgctgat ctcgatcccg gaactggaaa
     3241 cctgggtaga aacctgggcg ttctcagaaa cgattcattc ccgttcctat actcatatca
     3301 ttcgtaatat cgttaacgat ccgtctgttg tgtttgacga tatcgtcacc aacgagcaga
     3361 tccagaaacg tgcggaaggg atctccagct actacgatga gctgatcgaa atgaccagct
     3421 actggcacct gctgggcgaa ggcacccaca ctgttaacgg taaaaccgtg accgttagcc
     3481 tgcgcgagct gaagaaaaaa ctgtatctct gcctgatgag cgttaacgcg ctggaagcga
     3541 tccgcttcta cgtcagcttt gcttgttcct ttgcctttgc agaacgcgaa ctgatggaag
     3601 gtaacgccaa aattattcgc ctgattgccc gcgacgaagc cctgcacctg accggtactc
     3661 agcatatgct gaatctgctg cgcagcggtg cggatgatcc ggagatggcg gaaattgccg
     3721 aagaatgtaa gcaggagtgc tatgacctgt ttgtccaggc ggcacagcaa gagaaagact
     3781 gggcggatta tctgttccgt gacggttcga tgattggtct gaataaagac attctctgcc
     3841 agtacgttga atacatcacc aatatccgca tgcaggcagt cggtttggat ctgccgttcc
     3901 agacgcgctc caacccgatc ccgtggatca acacctggct ggtgtctgat aacgtgcagg
     3961 ttgctccgca ggaagtggaa gtcagttctt atctggtcgg gcagattgac tcggaagtgg
     4021 acaccgacga tttgagtaac ttccagctct gatggctcgc gttaccctgc gcatcactgg
     4081 cacacaactg ctgtgccagg atgaacaccc ttcccttctg gcggcgctgg aatcccacaa
     4141 tgtggcggtt gagtaccagt gccgcgaagg ttactgcggc tcctgtcgca cacgcctggt
     4201 tgcaggccag gtggactgga ttgccgaacc gttagccttt attcagccgg gggaaatttt
     4261 gccctgttgt tgccgagcaa aaggcgatat tgaaatcgag ttgtgatcgg gtactagcgc
     4321 cagatacgac gcttacgcgt attatctggc ctacaacgat tacatggcgt agtaatacgc
     4381 ttttacctgc tcccagtccg ctttggggat cggctccaga tatttttcca gctggcggaa
     4441 gtcatgatta atcgctttat cgcgacgcaa gcggcgacga cttttctcca gatcaagaaa
     4501 accagcttct gccttacctt ctgttttcac atagatatgg cgaacataac aacagccatg
     4561 ctgacgatta acgctatgca ttttcttaaa cgccagcgcc actgctttca acatggcttg
     4621 ccgtacttcg tcagaatagg gcgttactgc atgctgggca taccagtcag caatgctgat
     4681 gaaccccgcc atatcttcag tcaccagcaa cgcgcgccat tccccctcaa ttttcaccgc
     4741 ttcaccaaaa acgatcttcg gcacgatgac accggcccgt tccagttctt taattaccgc
     4801 aacctcacgg acaatcgttg gtcggccgaa aggataacgt acggaatgaa acagatgatg
     4861 cgtcatgcgc tttacatata gctttttgcc gttgcgctcg acgcattgca ccccgctcat
     4921 accattacgg cgatagttag gctcttcaac ccagtcgccc tctgttgccc accagtgatt
     4981 aaattcgtcg tgctttgctg aaactgccat accctatcgc ctgtcatttt ttattaacga
     5041 caatgactat aggtggttac ctgcgaaaaa tcttaatgaa acgtgtcgta ttaatgaatt
     5101 tcttttgcca ctactagcgc gacaccgctt tttcccttca tttgcagcgc atagactcca
     5161 ccagccacta ccactaatag tgcgcccagc agataaatca ttcggtagcc agtatgtgca
     5221 accatcatcc ccatcacaca ggctcccagc gccaggcctg catccatcgc attgaagaag
     5281 agggagtttg cgacaccgat tttatgcggt tccactgaac tgataatctg cgtctgaaaa
     5341 actggcgtca cggaaccata cccgatacca atcaacgcgc cagaaacgac catcattacg
     5401 ctgccctggg tgtagccgag aaccaccagc ccaaccgtaa agaacagcag gcaggggtaa
     5461 accacatatt tcgggccttt tttgtcgcaa acattgccgg tgaaggtacg gcagatcatc
     5521 aggaagatgg cgtagcagag caggaaatta ctggccgccg acatcagatt aagttcgcgg
     5581 gcgtaaagcg ccagaaaggc agaaaccccg gcatacgaaa aggtcataaa gaatgtaacc
     5641 atggcaaacg gcaatgccgc gcgatcgaac atggcggcga agcccatctt tggctttgaa
     5701 ccgtcggcat ggctgataac cggcggcacg gtcataatca gcgacaggat aatccccacc
     5761 gccgcaacgc cagtacataa ccagaaagcc atattaaagg cctcccagcg cgccatattc
     5821 aggccaatcc acggcccgac caccattgcc agccccattg ccagagagaa aaaactgatg
     5881 ccctccccac gtcgggaggc cgggatcagc cgcgcggaaa tcgttccctt taccgttgtg
     5941 ataaccccga aggtaatacc atgaagcacc ctaataaaaa gcagtgattc tattgagtgg
     6001 caaaaaggat acagcgcagt gaccaccaaa aaggccagag aagagagcac cagaatagtt
     6061 ttattcgaat atttacccac ccattgcccg gcaaagggtc gaataacaat cgccgcaatt
     6121 aaaaataaag tcaccaacaa acctgcttta tcaggagagg catgcaagcg gtcagagata
     6181 taaattggca acaatgttag taaaacataa aagacgaaga aaataataaa gctgatgatg
     6241 gttattgccc aaaaatcctt cgtccataac ttctctttca tactctaccg cctgtcagga
     6301 tgacgcacgc agacaacact cccgttactg cggttgcgtc aaattgtgat gtactttgca
     6361 gaacagaagt ataaatggcg gcatgctatt agagaaatta atcgttttaa tattattgat
     6421 tagcaaactc taatgactct tacccaaata aattctttac tcgccgtgct ggattacggt
     6481 ggctttactg aagccagcaa gcgattgttc atgacgcagt cggcggtaag tcaggcaatt
     6541 gctgccctcg aacaagaact tggtgtgaat atactgttac gcgatcgccg taaagctata
     6601 caactgacca cagcaggtca ccgcattgtg caacatctgc gcgccattaa tcgggaagtt
     6661 aacgcagtaa aagagatagc cgagcaggaa aagcagaacc ctcaaagaac gctgagactt
     6721 ggttgtttcc ccagcgtctg cgcctgtatc ttaccggttg tgattcgcta ctttgaaatt
     6781 catcatccga atataaaaat tattcctttt gaggaaaata gcacagcgat tattgattcg
     6841 cttcagaatg aatccattga tgccggtttt gttcattttc cggtgagcgg aatgtactcc
     6901 gtgcccattt atcaggataa attcaccgta gtgttaccgc ccaaccatgc tctggcaaaa
     6961 aacagcacta ttacagtgga agaattaatg ggtgaaccat taatcattag taaaggacgc
     7021 tatgaattaa gcattatggc actatttaaa gaaaagaata ttaccccaca gataaaatat
     7081 gaatttaatc acccggatac agcgatcagt tttattcgac agggattagg catcgcctta
     7141 ttaccagagt taacgctgaa aaccattgca gatgaattgt gttccgttcc tctcgaacca
     7201 accttctatc gacagatttc gttgctggct aaagaaaagc cggtagaagg cagtccactg
     7261 tttttactac aaatgtgtac ggaacaacta gtggtgagtg gaaaaatttg atgtaagaca
     7321 tgagattgat gacaaatgca agactgcctg attcgccaca cttagttgta ctatagcctt
     7381 taccaggcgt aataattcgt tttcacctga ttttggccca ctttgtgaat tggcgatata
     7441 tttatccagt tgggagaagt gacaatgact ccaggtagaa accggtatga agcgcccgac
     7501 tcactgtcaa aaccgattgt tgagagtaat gacactccgg cgaacagtac acagtttact
     7561 ctggagccat tctcaatctt caagtgggat acctaataac ttgtcaggga agttatcaa
//
LOCUS       DAZKIF010000181         7590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZKIF010000181 DAZKIF010000000
VERSION     DAZKIF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.182"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..3456)
                     /gene="rhsC"
                     /locus_tag="ACHOTW_004285"
     CDS             complement(<1..3456)
                     /gene="rhsC"
                     /locus_tag="ACHOTW_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFP9846278.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDITRY
                     RYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHRE
                     EGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVTV
                     HYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYG
                     SGWLAGMKLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSLQYDRD
                     YTWNDNGELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNRLPDPE
                     LHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQ
                     HRLVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWD
                     GDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGS
                     VVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLY
                     HCDHRG"
     gene            3685..4293
                     /locus_tag="ACHOTW_004286"
     CDS             3685..4293
                     /locus_tag="ACHOTW_004286"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP9846279.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            4391..5782
                     /gene="selA"
                     /locus_tag="ACHOTW_004287"
     CDS             4391..5782
                     /gene="selA"
                     /locus_tag="ACHOTW_004287"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP9846280.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            5779..>7590
                     /gene="selB"
                     /locus_tag="ACHOTW_004288"
     CDS             5779..>7590
                     /gene="selB"
                     /locus_tag="ACHOTW_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP9846281.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHL"
BASE COUNT         1482 a         2156 c         2260 g         1692 t
ORIGIN      
        1 gccgcgatgg tcgcagtggt acaggtggat ttttcgcgcc ggcgtgtata ccgggtccat
       61 ctggctttgc atctgcgcca cagtcaggcc gcacgatgcc agccagcggc ggctttcctc
      121 actcacccgg tcagccagga tttcactttc cagccggtcg agcatctgca ccagcaccgg
      181 cgggaacacc acactgccac cgtcttcgcc gccggactgc tgaagcgcat ccgccaggct
      241 gcggcgctgc gttttcgcca gctcaccggt ggcggtttca accctgatga gtggcgtgaa
      301 gctccccggc tgataaatcg tctggatgcg ggttctgtcg ttctgtattg tggtcagccg
      361 gtcgccgtcc cagccgtacc aggtcacttg cggtttccgt gacagcgaca tccagcccgt
      421 caggtcccgt tcacgtcgcc acacccgttt tgccaccctg cggcccagcg ggtcgtaaag
      481 atagcggctt tcgaccagcg gctcttcata ttgtgtccgc gtgtagtgca ccagccggtg
      541 ctgactgtcg taatggtacc ggtgagtccg ctcatcatcc gtgcggataa ccccttccgg
      601 gatgaggtcg gttttctctg tcagcctgcc gtgacggtca taccggtaaa gatagtgcgc
      661 gtcacgggcg atacggttat ccggccacat gctgagggtg ctgtccgggt gcagctccgg
      721 gtcgggcagg cggttacctg ccgggtccgt ggcatacggg atgcggatat ccagattcgc
      781 tgcggtggtg tgaacgccgg tcagcctgcc ggagtcgctg tagctgtaac tccgggtctg
      841 gcgcgggctg ctgatgcgga tgagttcgcc gttgtcgttc caggtgtaat cgcggtcata
      901 ctgcaggctg ttcaggtgct ggctctgtaa ctgcccggca ggggtataag cggtggtgag
      961 ttcataacgg ccgaatctgc gcagcgtttt ccggtgcagg cggtcgcggg tgaaatccac
     1021 cagcggtgtg tcgccgagct tcatgcctgc cagccagccg ctgccatagg tcagccattc
     1081 cacggcgggc aggctgtccg gtatacagcg gttcgccagc ccctgcgcgt tgtaagcgtg
     1141 tctggtctca tgctgccaga gcagtgcttc cgtctgcggg tgatgcaccg tctgacgctc
     1201 accggtcagc cggcctttct catcataccc gtaatgcacc gtcacccggt gcccttcgct
     1261 gatatggctg atgtctgtca gccagccgcg ttcgtcatac tgccagcgct ctgcggtttc
     1321 acccttcacg gtgcggtgcg tgaggcggtc tgcttcgtca tagtgccagt gggtgaccag
     1381 cccctcatcc tcgctgcgga taagtttgcc ggtcaggtcg tggtgataac gctgtgtgcg
     1441 gccgtcaaag ccggtttcct gtatcagccg gtcgagtaca tcgtaacgga aggtggtgtg
     1501 gctgccgttt tcactggtca ggcggatgac ccgtccggca gcatcgtatt ccatactgcg
     1561 cgtcagaccg ccctgcgtgg tacagatggc ttttccccac gcatcgtact gtgtcccgtt
     1621 tctgctgccg tccggggcaa tgacggtggt caggtcaccg gcggcgttgt attcataccg
     1681 cgtttcatgg ccctgcgtgt ctttcacggc aattaactgt ccacggctgt cgtatgcgcg
     1741 gtactgactc agcccttcct cgcggtgcac cgccgtcatc tgcccgaagc ggtcatggtc
     1801 ataacgggtt acataaccgg aacagtcggt gaagctcagc aactgaccgt aacggctcca
     1861 cgtcatggtt ttccggctgc cggtggcatc ttccgttgcg cagggtaagt cactgtgtgg
     1921 attatcataa cggtagcggg tgatatcgcc gtcaggggca gtttcctgaa tcagacggcc
     1981 ccattcatca tattcccggc gtatttccag cccgtcaggc ccggtggctg acgttaactg
     2041 gctgtggtgg ttatagtaaa acgccgatgc cctgccatcc ggcgtggtga tgcgcgtgat
     2101 gaggcccgtc accacatccg ggctgtattc tgttgtcctg cctgcggcat ccgtctgtgc
     2161 cctgagcctg cccaccgcgt caaactgact ctgcgtgacg ctgccgtccg cgtgttcctt
     2221 tttcaccacc cgcttcagcc cgccttcacc ctgcgtgtgc aggacttcac ggcggttcag
     2281 gctgtcggtg atggtgatgc ggtctttctc atactgatac gtgtagctta agcctgccgg
     2341 gtttagctgt tctgtcaccc gcccgtcgct gtcgtaacgg taacggattt ccggccggcc
     2401 cgtgtgacgg tgcgccacca tccggccccg gtatttatca tcgtaagtaa agctgcgcac
     2461 ctgtttgcca ctacggtcat acaccaccgc cagttcgccg cgcggcgtcc agccatagcg
     2521 caccagcggc gcggcaggta aattctccgg gtattccggg tcgtgcgtca gccacacggc
     2581 agacagacgg atgccgttgt cccggccata ttcggtgtaa cccggcaggg tatcaggaaa
     2641 agcggacggt tccgtcccgc cggaaatggc ctgctgccgg gcttcttctg cccgctgcgc
     2701 ctgcgtggtc agtaccagcc ggaagtgacg cccggcacca tccgtcacgc cggtgatttc
     2761 gccgctgaat tcaccggcgg cttcgcggtg gaacgtctgt gtgcgcccga agcggtccac
     2821 cagcccggtc agtacccggt acggcggcag cggcgcaggc agcacctcat ccgcttccgg
     2881 cacccgctca caccagccga gcagccacca cggcccctgc ggactgtttg tcgccagata
     2941 acgatgcgga cttaagcgga gttcttccgg cagcgcctgc cagagtgcgg ccagccggtg
     3001 accttcatcc agtttcgcca cgccgccgcg caccagccag agtgactcgc tgcggctgta
     3061 accgtcctca ccgggaaaca ggtgctcaaa atacaggctt ctgccgccgt tatcactgag
     3121 tatcagtgtg ttatcgcgca gctgtaagcg gatatccgca ggcattttcc agccggggcc
     3181 gaggctcccc accggcgcgg gcgtttttgt ccggtaactg ctgtaggtgc gggagaggat
     3241 gaacggcagc gggccgggca gggcgatgtc ggtttcaccg ggaaggacct ttgcaccgag
     3301 caggggattg accggatggc cggacgtcac tccgccgggg cacaccgaac aggccacgcc
     3361 ggtgggggca ccaatgcgca ccccggctga accctgaaca atgctaccgc catactgcgt
     3421 catgtcgccc tgacgcgccg ccggttttcc gctcatctgc tcatccttgt tttatcgggt
     3481 tatttgtctg cttccaggca aaaccctaca gatttataag aaagccttaa gtgagatatc
     3541 catcagatag aaacaattag gaaaatattt atatcaccat gataaatatg tgatttattt
     3601 ggaagtaaca aaccatcaac attttctgcc ctttttccca tcaccaggcc atactgatat
     3661 ttttcctcgc acaaaggact cggcatgaaa ctcgtcggta gctacaccag cccgtttgta
     3721 cgcaaacttt ctattctgtt gttagaaaag ggcataactt tcgaatttat taatgaactg
     3781 ccctataacg cggacaacgg cgtgacgcaa tttaacccgt taggaaaagt gccggtgctg
     3841 gtgaccgaag agggcgaatg ctggtttgat tcgccgatca tcgctgaata tattgaatta
     3901 atgaatgtcg ctccggcgat gttgccgcgc gatccgctgg agtcgttgcg ggtgcgcaaa
     3961 attgaggcac tggcggatgg cattatggat gccgggctgg tatcggtgcg tgaacaggcg
     4021 cgtccagcgg cgcagcagtc tgaagatgaa ttgttacgcc agcgggagaa aatcaaccgc
     4081 agtctggatg tgctggaagg atatctggtc gatggcacac tcaaaaccga tacggtcaat
     4141 ctggcgacta tcgccattgc ctgtgccgtc ggatatctca atttccgccg cgttgcgcca
     4201 ggttggtgtg tcgaccgccc acacttagtc aaactggtcg aaaacctgtt tagccgcgaa
     4261 agttttgctc gcaccgaacc gccaaaggct tgatgcgcga tatgtcctcc tgacccatct
     4321 cacgttacaa tccgtggtta tgttaaacgc ccttctccgt gtgagagggc cttgatcagc
     4381 caggtttcct atgacaaccg aaacgcgttc cctctatagt caacttccgg ctattgatcg
     4441 cttattgcgc gatagctcct tcctttcttt gcgtgatact tatggtcaca cccgcgtggt
     4501 ggaattgttg cgtcagatgc tcgacgaagc gcgagaagtg attcgtggca gccagacgct
     4561 gcctgcgtgg tgtgaaaact gggcgcaaga agtcgatgcc cggttgacga aagaagcgca
     4621 gagcgcgctg cgtccggtga tcaacctgac gggaaccgtg ctgcatacca accttgggcg
     4681 agctttacag gcggaagccg cggtggaagc cgttgcgcag gctatgcgtt cgccagtgac
     4741 cctcgagtac gatctggacg acgccggacg cggacatcgc gatcgggcgc tggcgcagct
     4801 gctgtgccgt attacggggg cggaagatgc ctgtatcgtc aataacaatg cggcggcggt
     4861 gttattgatg ttggcggcca ctgccagcgg aaaagaggtg gtggtatctc gcggcgaact
     4921 ggtggagatt ggcggcgcgt ttcgtattcc cgatgttatg cgtcaggcag gctgcaccct
     4981 acacgaagta gggaccacca accgcacgca cgcgaatgat tatcgtcagg cggtgaatga
     5041 aaataccgca ctgttgatga aagtacatac cagtaactac agcattcagg ggttcaccaa
     5101 agcgatagat gaagcggaac tggtggcgct cggcaaagag ctggatgttc ccgtagtgac
     5161 tgatttaggc agtggctcgc tggtcgatct tagccagtac ggtttgccga aagagccaat
     5221 gccgcaggag ttgattgcgg cgggcgtcag tctggtgagc ttctccggcg acaagttgtt
     5281 aggcgggccg caggcaggga ttatcgttgg taaaaaagag atgatcgccc gcctgcaaag
     5341 ccacccgctg aagcgtgcat tacgcgcgga taaaatgacc ctcgcggcgc tggaagccac
     5401 gttgcgtctt tatttacacc ctgaagctct gagtgaaaaa ttaccgaccc tgcgcctgct
     5461 tacccgcagc gcagaggtca ttcaaatcca ggcacaacgt ttacaggctc cccttgccgc
     5521 acattacggc gcggagtttg cggtacaggt tatgccatgt ctttcgcaga ttggcagtgg
     5581 ttcgctgccg gttgatcgcc tgccgagcgc ggcattaacg tttacacccc atgatggacg
     5641 cggtagccac cttgagtcat tagccgcccg ctggcgtgaa ttgccagtgc cggtgattgg
     5701 tcgtatttat gacggacgat tgtggctgga tttacgctgc cttgaagatg agcaacggtt
     5761 tttggagatg ttgttgaaat gattattgcg actgccggac acgttgacca cggcaaaaca
     5821 accttattgc aggcgattac tggcgtaaat gctgaccgtc tgccggaaga aaaaaagcgc
     5881 ggcatgacca tcgatctcgg ctatgcctac tggccgcagc cggatggtcg cgtgcctggt
     5941 tttatcgacg tacccggtca tgaaaagttt ctttccaaca tgctggcggg cgttggtggt
     6001 atcgatcacg cgctgttggt ggtggcgtgc gatgacggcg tgatggcaca gacccgtgag
     6061 catctggcga ttttgcagct gaccggtaac ccgatgctga cagtggcgct gaccaaagcc
     6121 gatcgcgtag acgaagcgcg tgttgatgag gttgaacgcc aggtaaagga ggttctgcgg
     6181 gaatacggtt ttgctgaggc aaaactgttt atcaccgcag caaccgaagg tcggggaatt
     6241 gatgccctgc gcgagcatct gcttcagttg ccggaacgcg agcacgccag ccaacatagt
     6301 ttccgcctcg cgattgaccg cgcatttacc gtaaaaggtg ccgggctggt cgtcaccggt
     6361 acggcgttaa gcggggaagt gaaggtaggc gattcactct ggctgactgg tgtaaataaa
     6421 ccgatgcgtg tacgtgcgct gcatgcgcaa aaccagccaa cagaaaccgc caatgccggg
     6481 cagcgtatcg cgcttaacat cgcgggtgat gcggaaaaag agcagattaa ccgtggcgac
     6541 tggctgcttg ccgatgtgcc gccagagccg ttcacacggg tgattgtcga gcttcaaacc
     6601 catacaccgc tgacccagtg gcagccgctg catattcacc acgccgccag ccacgtcacg
     6661 ggacgcgttt cactgctgga agataacctt gctgaactgg tcttcgaccc cccgttatgg
     6721 ctggcagata acgaccgcct ggtattgcgc gatatctctg cccgcaacac gctggccgga
     6781 gcgcgcgtcg tgatgcttaa cccgccgcgt cgcggtaaac gtaaaccaga gtatctgcaa
     6841 tgcctggctt ccctcgccag ggcacagaat gatagcgaag cgttagccgt tcacctgcaa
     6901 cgcggcgcgg ttaatcttcc tgatttcgcc tgggcgcgcc agctcaacgg cgaagggatg
     6961 cgcgaactgc tgcaacagcc tggctatatt caggctggtt atagcttgtt gaatgcgccg
     7021 gttgccgctc gctggcaacg gaaaattctc gacacattag cgacttatca tgagcaacat
     7081 cgcgatgaac ctgggcctgg gcgcgaacgt ctgcgacgta tggcgttgcc aatggaagat
     7141 gaagcgctag tactgttgct gattgaaaag atgcgcgaaa gcggcgacat cctcagtcat
     7201 cacggctggc tgcatctgcc ggatcacaaa gcgggcttca gcgaagagca gcaggccatc
     7261 tggcaaaaag cagagccgct gtttggtgac gagccgtggt gggtgcgtga tctggcaaaa
     7321 gagacgggta ccgacgagca ggcaatgcgc ctgacgctac gccaggcggc gcagcaagga
     7381 ataattaccg cgatcgtcaa agatcgttat taccgtaacg atcggattgt cgaatttgcc
     7441 aatatgatcc gcgatctcga tcaggagtgt ggttcaacct gcgcggcgga tttccgcgat
     7501 cgcttaggcg taggccgaaa gctggcaatt cagattctgg aatattttga ccgcattggc
     7561 tttacgcgtc gtcgtggaaa tgatcattta
//
LOCUS       DAZKIF010000182         7573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZKIF010000182 DAZKIF010000000
VERSION     DAZKIF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.183"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            207..5635
                     /locus_tag="ACHOTW_004289"
                     /pseudo
     CDS             207..5635
                     /locus_tag="ACHOTW_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5751..6815
                     /gene="hipA"
                     /locus_tag="ACHOTW_004290"
                     /pseudo
     CDS             5751..6815
                     /gene="hipA"
                     /locus_tag="ACHOTW_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            7086..7355
                     /locus_tag="ACHOTW_004291"
                     /pseudo
     CDS             7086..7355
                     /locus_tag="ACHOTW_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2058 a         1598 c         2080 g         1837 t
ORIGIN      
        1 taactcatag ttcattttta aataaaataa gaactatgca atttttaatc tatcaattcg
       61 aaagtattag caagttagcc aaattgacac tttcccacct ccaactaaca ctcaatacag
      121 acctctgaca atttaataaa tttcattttt aatgtctgac gactcatctc tttcgtggct
      181 gtattgtttg tatttggagc gtttatatga atagaatcta tcgcgtgata tggaattgca
      241 ctctacaggt atttcaggcc tgctcggaat taactcgcag ggtaggtaaa acatcaacgg
      301 ttaatttgcg aaagtcctct ggactgacaa cgaaattcag tagattgacg ctgggtgttt
      361 tgctggcact aagcggttca gtgtctggtg cgagtctgga agttgataat ggtcagatta
      421 ccaatattga tactgatgtt gcttatgatg cctacctggt tggctggtat ggcactggag
      481 tgcttaatat tttggctggc ggtaatgcct ccttaaccac tattactacc agcgtcattg
      541 gcggtaatga ggattcggag ggtaccgtta atgttttggg tggcacctgg cgattgtatg
      601 atagcggaaa taatgcaagg cctttaaatg tgggtcaatc cggaacgggg acgctgaata
      661 ttaaacagaa gggtcacgtc gatggaggct atttaagatt aggtactcaa gctgcaggcg
      721 tcgggacggt taatgttgag ggagaggact cggttttgac gaccgaatta ttcgaaatag
      781 ggagttacgg tacgggctca ttaaatatta cggataaggg ttacgtcacg agttcaatag
      841 tcgccatttt aggatatcaa gcgaacagta atggtaaggt tgtcgttgaa aagggtggcg
      901 agtggctaat aaaaaataat gattcctcaa ttgaatttca aattggtaat caaggaactg
      961 gggaggcgac tattcgcgag ggtgggctga ttacggctga aaatacgatt atcggtggca
     1021 atgccaccgg tgtcggaacc ctgaatgtgc aggatcaaga ctctgtcatc acggtacgca
     1081 gactctataa tggatatttc ggtaatggcg cagtcaatat ttccaataat ggactaatta
     1141 ataacaaaga atattcattg gtgggcgttc aggacggttc ccacggtgtc gtcaacgtga
     1201 ccgataaagg gcattggaat ttcctcggaa cgggcgaagc tttccgctat atctatatcg
     1261 gtgatgctgg cgacggtgaa cttaatgtct cgcgtgaagg taaagtagat tcgggaatta
     1321 tcactgcggg gatgaaagaa acaggcacag gcaaccttac tgttaaggat aagaactccg
     1381 ttatcactaa tctcggaact aatcttggtt atgacggcca cggcgaaatg aatatcagta
     1441 atgaggggct tgttgtcagc aacggaggaa gctctctcgg ttatggagaa accggcgtcg
     1501 ggaaggttag catcaccacg ggggggatat gggaggtcaa taagaatgtc tataccacca
     1561 ttggtgttgc gggcgtcgga aacctcaata ttagcgatgg cggtaagttc gtatcgcaaa
     1621 atattacttt tttgggcgat aaagcaagcg gtatcggcac actgaacctg atggatgcga
     1681 catcgtcgtt cgatactgtg ggtatcaatg tcggtaattt tggtagcggt atcgtaaatg
     1741 tcagtaatgg tgccaccctt aattcaacgg gctatggatt tatcggagga aatgcctccg
     1801 gtaagggaat agttaatatt tcaacggata gtctctggaa tttaaagacg tcttctacta
     1861 acgcccaatt gctacaggtc ggtgtattag gcacgggtga actgaatatt accaccggag
     1921 gtatagttaa agcgcgtgat acacagatag ctctcaatga caaaagtaag ggcgacgtga
     1981 gggtggatgg gcagaactct cttcttgaaa cattcaatat gtacgtaggg acatctggta
     2041 cgggtacgtt aaccctgacg aatagcggca cgctgaatgt cgaaggtgga gaagtttact
     2101 taggtgtttt tgaacctgct gtaggaacgc taaacattgg tgcagctcac ggtgaggcgg
     2161 cggcagatgc cggatttatc accaacgcga cgaaagtaga gtttggctct ggcgaaggtg
     2221 tttttgtctt taatcatact aataacagtg atgccggcta ccaggtcgat atgctgatta
     2281 caggtgacga taaagacgga aaagtgatcc atgatgcagg ccatacggtg ttcaatgcag
     2341 ggaatactta tagcggtaaa acgctggtca atgacggcct cctgaccatt gcgtctcata
     2401 cggcagatgg ggtaacgggc atggggtcga gtgaagtaac cattgcaagc cccggtacgc
     2461 tcgacattct cgcatcaacg aacagtgcag gagattacac gctgaccaat gcgctcaaag
     2521 gcgatggctt gatgcgagtt cagctgtcat cctccgacaa gatgtttggc tttacacatg
     2581 caacagggac tgaattcgcc ggtgttgccc aactgaaaga cagtaccttc actctggaac
     2641 gcgacaacac cgctgcgctt actcacgcga tgttgcagtc tgacattgaa aataccacat
     2701 cggtaaacgt gggagagcaa tccattggtg gactggccat gaatggcggt acgctcattt
     2761 tcgatacgga tattcctgct gcgacgcttg cagagggata tatcagcgtc gatacgctgg
     2821 ttgtcggcgc gagtgactac acctggaaag gccgtaacta tcaggtaaac gggacgggcg
     2881 acgtgcttat cggcgtgcct aaaccgtgga atgatcctat ggcgaataac cctctgacga
     2941 cgctcaattt gctggaacac gatgataacc atgtcggcgt tcaactggtg aaggcgcaaa
     3001 cggttattgg gtcgggtggc tcattaacgt tacgtgattt acagggcgac gaggtggaag
     3061 cggacaaaac gttacacatt gcgcaaaacg gaacggtggt cgccgagggt gattatggat
     3121 tccgcctcac gaccgcacca ggtgatggtt tgtacgttaa ctatgggctg aaagcgctga
     3181 acatccatgg agggcaaaag ctgacgttag ccgaacatgg cggagcctat ggcgcaacgg
     3241 ccgatatgtc ggcaaaaatc ggtggtgaag gggatctggc aatcaatacg gtgcgacagg
     3301 tttcgctttc caacggtcag aacgactatc agggggcaac ctacgttcag atggggacat
     3361 tacgtaccga tgcggatggc gcgctgggca acacccggga actgaacatc agcaacgcag
     3421 ccatcgtcga tcttaatgga tcgacgcaga cggtagagac attcaccggg cagatgggtt
     3481 cgactgtttt gttcaaagaa gggtcgctga cggtaaataa aggtgggatc agtcagggtg
     3541 aactgacagg tggcggaaac ctgaatgtta cagggggaac gctggctgtc gaggggctta
     3601 atgcacgcta caatgcgtta accagcgtta gcccaaatgc ggaagtcagc ctcgataata
     3661 cgcaggggtt aggcagagga aatattgcca atgacggtct gttaacgcta aaaaacgtga
     3721 ctggcgaact gcgtaatagc ataagcggga agggtatcgt gagcgcaacc gccaggacag
     3781 atgtagagct ggatggcgat aatagccgct ttgtggggca attcaacatt gatacaggca
     3841 gcgcgctcag cgtcaacgag cagaaaaacc tgggtgatgc ttccgttatc aataatggcc
     3901 tgctcaccat ctccactgag cgtagctggg cgatgacgca cagtatcagc ggtagcggtg
     3961 atttgacaaa actgggtacc gggatcctga ctcttaacaa cgattcctcg gcgtatcagg
     4021 gtacgacgga tatcgtgggg ggggaaattg ctttcggttc cgactctgcc attaatacgg
     4081 caagtcaaca cattaatatc cataacagcg gtgtgatgtc gggaaatgtc accactgcag
     4141 gtgatgtgaa cgttatgtct ggggggacac tgcgtgtcgc taaaaccaca atcggcgaat
     4201 cggcggcaac ctggagaatg gcggcacggt tcaaatgaac agtgaaggag gaaaaccggg
     4261 gaatgtactg accgttaacg gcaactatac cggaaacaat ggcctgatga cgttcaacgc
     4321 gacgctgggc ggcgataatt cacccaccga taagatgaac gtgaaaggcg atacccaagg
     4381 gaacactcgc gttcgggttg ataacattgg cggcgtcggt gcgcaaacgg tcaacggtat
     4441 tgaactcatt gaggttggcg gtaattctgc aggtaatttc gcgctgacca ccggaactgt
     4501 cgaagctggg gcttacgtct acacgctggc taaagggaag gggaatgacg agaaaaactg
     4561 gtatctgacc agtaaatggg acggcgtaac gccagcggat acacccgatc ccatcaataa
     4621 tccccctgtt gtggatccgg aaggcccatc agtttatcgc ccggaggccg gaagctatat
     4681 cagcaacatt gccgcagcca actcgctgtt tagccatcgc ttacacgacc gtctgggtga
     4741 gccgcaatat acagattcac tgcattctca ggattcagca agcagtatgt ggatgcgtca
     4801 tgtcgggggg cacgaacgtt ccagtgccgg agacggccag ctaaatactc aggctaaccg
     4861 ctatgtattg cagctaggcg gcgatttggc gcagtggagt agcaacgcgc aggatcgctg
     4921 gcatcttggc gtgatggcag gctacgccaa tcagcacagt aatactcaga gtaatcgtgt
     4981 gggttataaa tcggatgggc gcatcagcgg ttacagcgct gggctgtacg cgacctggta
     5041 tcagaacgat gcgaataaga ccggcgctta tgttgacagc tgggcgctgt ataactggtt
     5101 tgataacagc gtcagttccg ataaccgttc tgctgacgac tatgattctc gcggtgtgac
     5161 ggcctctgtt gagggtgggt atacctttga agcgggaaca tgtagcggca gcgaagggac
     5221 gctgaatacc tggtacgtcc agccacaggc gcaaatcacc tggatgggtg tgaaagattc
     5281 tgaccatgcc cggaaagacg gaacgcgcat tgaaacggaa ggcgacggaa acgtgcaaac
     5341 gcgacttggg gtgaaaacct acctgaatag ccatcaccag cgtgacgatg gtaaacagcg
     5401 tgagttccag ccttacattg aagcgaactg gatcaacaat agcaaagtct acgccgtgaa
     5461 gatgaatggt caaaccgtaa gccgtgatgg tgcgcgaaat ctcggtgaag tacgtaccgg
     5521 ggttgaggcg aaagtaaata acaaccttag cctgtggggg aatgtcggtg tgcaactagg
     5581 tgataaaggc tatagcgata ctcagggcat gctgggagtg aaatatagct ggtaaacagt
     5641 ataagccgca tgtcgagatg gcatgcggct taatattgcc aacttcaaaa ggcttttaaa
     5701 gcctcccttt tatccgcgat cgcggatatc gcagcgttta tcccgtacag tttgatttat
     5761 tgtcagaaat agggcgagac agcgttggtg ccgtgacgtt aatacccgaa gacgaaaccg
     5821 taacgcgtcc gataatggca tgggaaaagc ttactgaagt cagacttgaa gaagtattaa
     5881 cggcttataa agcagatatc ccgctaggca tgattagaga agaaaatgac tttcgcatct
     5941 cggttgctgg cgcacaggag aagacagcac tgctcagaat aggcaatgac tggtgcattc
     6001 cgaaaggaat aacgccgacg acgcacatca ttaaattacc gattggggaa atcaggcagc
     6061 ccaatgcgac gctcgatctc agccaaagcg ttgataatga gtattactgt ctgctgctgg
     6121 cgaaagaact tgggttgaat gtcccggacg cagaaatcat taaagcggga aatgtgcgcg
     6181 cgttagcggt cgaacgtttt gacaggcgtt ggaatgctga gcgaacggtt ttacttcgct
     6241 tgccacagga ggatatgtgt cagacattcg gtttaccttc atcggtgaaa tatgaatcag
     6301 atggaggccc aggcatcgcg cggatcatgg cttttttgat ggggttcagc gaggcgcttc
     6361 gcgatcgtta tgattttatg aaattccagg tcttccagtg gttgattggc gcaacggatg
     6421 gccatgcaaa aaacttctcc gtatttattc aggctggcgg cagttatcga ctcacgccat
     6481 tttacgacat catttcagca tttccggtcc ttggcggtac gggaatacac atcagcgatc
     6541 tcaaactggc aatggggctt aacgcatcca aaggcaaaaa aacggcaatc gataaaattt
     6601 atccgcgaca ttttttggcg acagcaaagg tgctgagatt cccggaagtg cagatgcatg
     6661 aaatcctgag tgactttgcc agaatgattc cggcagcact ggataacgtg aagacttcat
     6721 taccgacaga ttttccagag aacgtggtga cggcagttga aaccaatgtg ttgaggttgc
     6781 acggtcggtt aagccgagaa tacggtatta agtaagagta agatatgggg tttggtcgtt
     6841 gttaatgagc aggacaatca tgaccgccaa cgactagatt ctcccttggt ttatattggt
     6901 gcgcataatg tatattatgt taaatcgtat ctgagtgata cgaagtgatt ttccggtagt
     6961 cgttattcgt tcaggattcc agaacttaag cttctcctct ttcataattg gcctgtgcat
     7021 gacggatata ggctggctgg tatgccgatt tgccgtttgc cgcatcttcc cagcgttgaa
     7081 gactcaagct ggcaaaccgc gtgcaaatga gcacgccctc tttccatcaa cacttcaggc
     7141 agcttgcggg aatgagtcca ctccggtatc agaaatggct acgtctcaat gaagtcaggc
     7201 gaacgatgct gaatgaacat tacgatgtca ccacagcagc ctatgctgtc ggttacgaaa
     7261 gcttatccca tttccgacgg gaatatttgc ggatgtttgg agagtcacct aagagagata
     7321 ttaccaggtt aagaaagtct gtcggtcagc tttaataaac cctgcactta tctgtttaga
     7381 gcaattcggt gttagttaca ccaggcaaac attaactata gctaatgatt tatagccata
     7441 taaaccatta gtagcccttg acctttcctg acacttccct tgttcgcaat aatattctgt
     7501 ttagtcacgg tttataaata acatgcataa tgaaatcgtc atgttattta gcgacataaa
     7561 atgtttttgc aaa
//
LOCUS       DAZKIF010000183         7542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZKIF010000183 DAZKIF010000000
VERSION     DAZKIF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.184"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(342..614)
                     /locus_tag="ACHOTW_004292"
     CDS             complement(342..614)
                     /locus_tag="ACHOTW_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP9846282.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(620..1171)
                     /locus_tag="ACHOTW_004293"
     CDS             complement(620..1171)
                     /locus_tag="ACHOTW_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFP9846283.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMEK
                     PADYARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQ
                     SSYTPAQQLRFQQRLDYINGMQE"
     gene            complement(1168..1920)
                     /locus_tag="ACHOTW_004294"
     CDS             complement(1168..1920)
                     /locus_tag="ACHOTW_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFP9846284.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
     gene            2854..3114
                     /locus_tag="ACHOTW_004295"
     CDS             2854..3114
                     /locus_tag="ACHOTW_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9846285.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
     gene            3111..3668
                     /locus_tag="ACHOTW_004296"
     CDS             3111..3668
                     /locus_tag="ACHOTW_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9846286.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTHPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            3665..3886
                     /locus_tag="ACHOTW_004297"
     CDS             3665..3886
                     /locus_tag="ACHOTW_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846287.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAALQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            3886..4209
                     /locus_tag="ACHOTW_004298"
     CDS             3886..4209
                     /locus_tag="ACHOTW_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFP9846288.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            4223..6556
                     /locus_tag="ACHOTW_004299"
     CDS             4223..6556
                     /locus_tag="ACHOTW_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFP9846289.1"
                     /translation="MSGMKVSQAEKAARGHWSRILPALGVNVLKNRHQPCPVCAGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVSKVLDVGISEAADRINGIIGNLLPVSQGMLES
                     GSPEKEDGRKAAAVLAARLFDKSRQTTGNAYLTSKGFPALPCRELTAMHKVGGVAFRA
                     GDLVVPLYADGELVNLQLINANGGKCFLKGGQVKNAFYLVEGTAKAAKRLWIAEGYAT
                     ALTINYLTGDAVMVAFSSVNFLSLASIACSEYPTHQIIIAADRDLNGAGQTRGAAVTG
                     ACNCTMALPPVFGDWNDAFTQNGEEATRHAIHEVIKPAVASPFDTMSEAEFTALSVSE
                     KAQRVVDHYKNSLAVDPNGQLLSRYEAGAWKVIYYADFARDVAALFQRLDAPFSSAKI
                     ASLVETLKLIVPQQQNPARQLIGFRNGVLDTRTGLFSPHDKKHWLRTLCEVDYTQPVD
                     GESLETHAPAFWRWLDRAAGFNPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIEMLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPDQIAPEERD
                     TQLKEKIASELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADSAFDFCGYL
                     EVLPDTTGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPLMLKEY
                     GLQYEKRRTNQGMQTNLALREESNADWLPKCDEFAAK"
     gene            complement(6689..>7542)
                     /locus_tag="ACHOTW_004300"
     CDS             complement(6689..>7542)
                     /locus_tag="ACHOTW_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846290.1"
                     /translation="LINKNIHGAGRNIVDFIDLGNWDFISNFSFDDSTRRLELEWHPN
                     DKFHIYIESVVFVEFNDTIYAFLKGYYHNQLSLNRIYNTKCSSCSFENSGSYMVDVYR
                     TVKRVNETIQTPNINCYTTCILTRPANGHVTSTGFSRNLMDAINISLAEHKIASLHNE
                     VMSIEEYDRDSLQEKGNTARRYLEYILMLVNIRIMHLNNVQYQEQMLGSLVSVIEALD
                     YEPLMKNDVEITKDILNACSHHGGVRIEKKDVIFSLEVIENLIKAIKKTDINKLQLDG
                     MFKSIQK"
BASE COUNT         1840 a         1769 c         1834 g         2099 t
ORIGIN      
        1 tctaaataat ttgcaaaatt attttgggta ctttcgcctc tacacttcct tcgggtggtt
       61 ccacaataat ggatctcacc ggcgtttgat attttataat ctaacccctt agcgcgcgca
      121 gccccccacg cctgctcgct tcgcttaaca gactggtttt catgcattcc gcaaatcgtc
      181 tcagaagcca ccacacaagg gctttcgcgt caaaaatggt gcatgagaat catgcgtttt
      241 catgcgctat agacatgcac tcatgcgctc tcaggccagc cagggaaaag gcgtaaaaaa
      301 tcccagtact ggaccgggat tacgtgggct ttttttgcta atcagacagg aatttgctga
      361 cggcttgaca gctttgacgc ggagccatag cgattgagag tttgctgttg tttttccggc
      421 atttgtgctt tctctgattc ctgttcattg cgccggggct tcatgattat cgtatccaca
      481 ctttccagtg cggtaaatgt gtaggaacat tcaagattct ggcactgata ccatgagcgt
      541 ttaaccgatg gtgcttcata ggcggaggtt ctggcgtgtg ccgtcgtacc gcattcggga
      601 catttaagtg ccattaatat tactcctgca tgccgttaat gtaatccaga cgttgctgaa
      661 agcgcagttg ttgtgcaggg gtgtaagagc tctggcaatc acgaaccatc atggggtcag
      721 gcatcacacc ggattcatcc agaatcatac gataatcccc ggcaatttca ggcggtgtta
      781 ttgccagctg gcgaaccaga gcattgcgta atatacggga cgcaacgtca acccctccgc
      841 gccccttcag gaacggagcc agtgcagatg tcagggctgc tccattcgcc tgcataaaat
      901 tgctgagagc gtcttctgtg caggcctcca tcagttgatg ctgcgagtaa atgcattcgc
      961 gcgcagcaca gttaatttgc catttcagca catccagatg ttcccgctgt aattcgagct
     1021 gtcgagcata atctgctggt ttttccatgg cagtgaggag tgattctatc tgttgttcaa
     1081 cggcttttcg gctctcacta tgtccagccc acagaatacg ggcatttcta aaactgataa
     1141 gcgcttggtc tggagtgact cgggccatca tactgctttc tccttctcag cacgttcctg
     1201 acgaaccttc attttatgca tagctgctgg agagagttta ttactgaaat ccagacgggc
     1261 cgcataatcc ggggcaactc ccgccagttt aaacacgggg tcctgttcgg gcatcgcatc
     1321 gttagtcaga gccggtctgg tgatatgttt atgaataaaa tctttcagca gtgtgtccgg
     1381 gtcattaact gaatgcacca caccgacaac tgcgctggct tccctgccca ttgttgattt
     1441 gagcaggctt agtgtctgga ttagtgcttt tccatgcgac tgcataaaat cttcccagat
     1501 ttgttttgcg cgaataccca caagggtgtc gtgcgcgtgg atatactttc cggctaattc
     1561 cgcagcctcc tgcggcaaaa gtgcattttc gctttcctga gccgccagca gctcatcaaa
     1621 atcctcaagc gtttctcgtc ccagcgcaat ttccgtgcgc agttttttca tttcctttga
     1681 gactacgccc tgactttcac gaaatagcgt gcgccactcc tcattcaggg cgttcgtcgt
     1741 ggcttccatt tcagcgcggc gctggcggat tgtcgcaata ttatccgctg ccgctttttg
     1801 ctgacgacgt gcttcaagcc atgcagattt ggtggcattc actttctcaa gcgcctgttg
     1861 cgtcgaaaca gggatagaaa tttcaacgat cgggttattg ttctgttctg ggattgtcat
     1921 gtcagcacct agtggtttgt cgtggtgtca attgtgctgt gatgcataca aagctgccat
     1981 tgaacgccat tgtgcgagcg acaagacaaa gactgaaccg ccccggacct cttttggcta
     2041 gccagaaaag gcctcgttta actaaggctg tttcagactt accttaacta ttcactacta
     2101 ttcacttagg taaaaaaaat aggtaataca gaaggttata aggtgaaggg ttggcatggc
     2161 agtattcatc gactgttcat aactgttcac atgggaataa aattatttat ggtatttttt
     2221 cataatgggt ggttttttta ttcctcgtag ctatttgttt tttgctcctc accactattc
     2281 acccttattc aggagtattc aacaggcaca taaatagact gcatgttttt gccggggaat
     2341 aacattttcg cctctggcca tggtgttgca tttcatactg atttttataa aactagatct
     2401 gtccggttct ttatggattc attcggatat attttacgat ttaaggtgat gttatttaat
     2461 atcaaggttt ttatgcaggc ccaatgttta taagttggat gcgattcaaa aatatgcccg
     2521 gaaattgggg cctgcaatgc aaggcctcgt gtagaagttg tactgccttg ctgtattggc
     2581 ggtggggtta gcactatcag gtgcaatgac ccataggata gtaaaccctg ggtgaatggt
     2641 agttcctatt aatgctcctg tatttatagt ccgcaggcat ggtatttatt atgtcatttt
     2701 atatggttta atcgggcttt aaattccata aaatcaatat gtgacgctaa ataaaaaatg
     2761 cgtgcgcagg tggtttatat acacataata aggaactacc tgaacccgga taaattcatc
     2821 cggactgttg tggaaattaa agagggtaga taaatgcgta atatttctgt gtctgcgcca
     2881 gctcctgctg cgccattatt tcctgtatcg aaccatcatg agcgattttt acgcctgcct
     2941 gaggtgatgc acttatgcgg gttgtcccgt tcgaccgttt acgacctgat cagccgcgat
     3001 gcgtttccga agcagatccc gcttggcggt aaaaatgtcg cctgggcaca gtctgaggtc
     3061 agcgcatgga tggcggaccg catcagcgcc cgtgggcggg gatgtgatgc atgatgatac
     3121 ctgcatacat taaatacctt tattctggct tgcttactgt cgtcatttcc aggtatagtt
     3181 tttccgctgt cgcaaaatcg gcagccggaa ttggcgttcc gtataactta ttggcgacaa
     3241 ttgacgcccc ttgcgtcttt ttttacgtcg tagctcaggc acaaccattt tcagggctgt
     3301 ggtgtttatg cttacaccat ggttctatcg agataatggt agtccgggcg gggcagcctt
     3361 cgggctggcc ggtttccaat aaggccggtt acgccaaccc cgttcgggct gccaccagtg
     3421 aaattggcgt ttccggtggt agcaataacc gctacttatt ggaggctgcc atcatggcta
     3481 caatcctcac cccgtcacac ccgcaatatg tctttgtgtt tgccgcaatc cgccgtgctg
     3541 acactcaccc ccgcatctgt atgcttcgca ctgtctcctg cgatgagcgt tccgcgcgcc
     3601 gtttgctagt ccgagattat gtactctccc tttctgcacg gctgcctgcc ggggaggtga
     3661 cgctatgaac cagtttgaga tttcctacga cgatgttgta aggctgaaac atttacgcaa
     3721 tgtgggtgag tatgtaactg gcatggcagc cctgcaggac tgttatgaaa agccagcagg
     3781 tgcccaatgc gaacaactgg tttccctcat ttatctgatg acagagcaac tggatggagt
     3841 ggtgcaacgc tgccatgacg acctgatgaa tgcggaggtg gcctgatgat atgctgtgaa
     3901 tctcttctgg cacttcgtgc cgctctgtat cgccgtgccg ttgcctgtgc ctggctggct
     3961 ctaagcaatc atcaggaacg ttattccggc ctgacgctcg ctgaacttga agatgccata
     4021 gcccgcgagc tggaagggtt ctatctgcgc cagcatgggc agcaaagagg gctggaaatt
     4081 gcctgcgcgt tgctttcgga tctgatggag tcggggccgc ttaaagcctg tccggttctc
     4141 tcactgctcg gaatgacggt catggatgaa ctttgttccc gtcacctcaa caaaccagcg
     4201 ctgcactaag gagggccgca caatgtcagg aatgaaagtt agccaggctg agaaagcagc
     4261 tcggggtcac tggtcaagaa ttttacctgc gctgggcgta aatgtactga aaaatcggca
     4321 ccagccctgc ccggtctgtg ccgggaaaga ccgctttcga tttgatgacc aggaagggcg
     4381 gggaacgtgg ttctgtaacc agtgcggggc aggtgatggc ctggcgcttg taagtaaagt
     4441 actggatgta ggcattagtg aagcggcaga cagaataaac ggcattatcg gaaacctgct
     4501 gccagtatct cagggaatgc ttgaatctgg ttctcctgaa aaagaggacg ggagaaaagc
     4561 tgcagcagtg ctggctgccc gtttgtttga taagtcccgc cagaccactg gcaatgccta
     4621 tctgacgagt aaagggtttc ctgcactgcc ttgccgggaa ttaaccgcta tgcataaagt
     4681 cggtggtgtg gcatttcgcg cgggagatct tgtcgttcca ttgtatgcag atggagagct
     4741 ggtaaatctg cagttaatca acgctaatgg gggcaaatgc ttccttaaag gcggtcaggt
     4801 taagaatgcc ttttacctgg ttgaaggtac tgccaaagca gccaaacggc tctggatagc
     4861 ggaaggatat gccaccgcac ttactatcaa ctatctgact ggcgatgctg tcatggtggc
     4921 cttttcgtcc gtcaatttcc tttccctggc gagcattgcc tgcagtgagt acccaacgca
     4981 ccagataatt attgctgctg accgcgatct caacggtgcg gggcaaacaa ggggcgcagc
     5041 tgttaccggg gcctgcaatt gcacaatggc gctcccgcct gtgtttggtg actggaacga
     5101 tgcattcacg caaaacggcg aagaagccac ccggcatgca attcatgaag taataaaacc
     5161 agctgttgcc agccccttcg acacaatgag cgaagctgaa tttaccgcgc tgagcgtcag
     5221 cgaaaaagcg cagagggtag tggatcacta taaaaattca ctggcagtag acccgaacgg
     5281 gcagctcctt tcacgctatg aggcgggggc ctggaaagtt atctattacg ccgattttgc
     5341 ccgtgatgtc gctgcgctgt ttcagcgcct cgacgcacct ttttcatccg cgaaaattgc
     5401 gtctctcgtg gaaaccctca aactgatcgt tccgcaacag cagaatccgg cgcggcaact
     5461 tatcggattt cgcaacggtg tgctcgatac ccggacagga ttgttcagcc cgcacgataa
     5521 gaagcactgg ttacgtacgc tgtgcgaggt ggattacacg cagcccgttg acggtgagtc
     5581 actggaaacc catgccccgg cattctggcg ctggctggat cgtgccgcag gttttaatcc
     5641 tgaaaaacgg gacattattc tggctgcatt gtttatggtg ctggctaacc gttatgactg
     5701 gcagctgttt ctggaggtca ctggccctgg cggaagtgga aagagtattc ttgctgaaat
     5761 agcaaccatg ctggcgggtg aagataacgc gacctcggca accattgaaa tgcttgagtc
     5821 gccaagagaa cgagctgcgt taataggttt ttcactgatt cgacttcccg accaggaaaa
     5881 gtggagcggt gacggggccg gactaaaagc catcactggc ggcgatgcgg tatccgttga
     5941 tcccaaatat cagaacgcct attcaaccca catcccggcg gtcatcctgg ctgtgaacaa
     6001 taatccgatg cgcttcactg atcgtagtgg tggagtttca cgccgaaggg tgatcctgca
     6061 tttccccgac cagatagccc cggaggaacg cgatacccag ctcaaagaga aaattgccag
     6121 tgagctagcg gtgattgttc gccagcttat gcagcgtttc agcgacccaa tgagtgccag
     6181 gacattgctt cagtcacagc agaactccga tgaagcgctc accatcaaac gtgatgctga
     6241 ttcagctttt gatttttgcg gctaccttga ggtcctacct gacaccacgg gcatgtttat
     6301 ggggaacgct aatattgttc cacgtcagcc tcgaacttac ctctaccatg cctatctggt
     6361 ctacatggag gctaacggct ataaaaatac gctcagtctg accatgtttg gcaaggggct
     6421 accgttaatg ctcaaggaat atgggctgca gtatgagaaa cgacggacca atcaaggaat
     6481 gcagactaat ctggccctaa gagaggaaag caatgctgac tggttgccaa aatgcgatga
     6541 gtttgcagcg aaataactga cctaagaccg gctgtgccgg tcttaaatcc gctacaggaa
     6601 attatctcaa aatatatatc gataaaattt aaaagttata tgctaaggcg tcagcggaaa
     6661 atgcatctaa ttcattgtta gatgatgttt atttttgaat tgatttaaac atcccatcga
     6721 gttgtaattt atttatgtcg gttttcttta tggctttaat taagttttca ataacttcca
     6781 aagaaaaaat tacatctttc ttttcaattc taaccccccc gtggtggctg caagcattaa
     6841 ggatgtcctt tgtaatttca acatcatttt tcattaatgg ttcatagtcc agagcctcaa
     6901 taacagaaac taaactacct agcatttgct cttggtattg aacgttgttt aaatgcatta
     6961 tacgaatatt aacaagcatg agaatatatt ctaaatatct cctggcagta tttcctttct
     7021 cttgtaaaga atcacgatca tattcctcaa tactcatgac ttcattatgt aaagaggcta
     7081 ttttgtgctc tgctaatgaa atatttatcg catccatgag gtttcgtgag aagcctgtag
     7141 atgtaacatg accatttgcg ggccttgtta aaatacatgt agtatagcaa tttatatttg
     7201 gggtctggat tgtctcattt actcttttaa ccgttctata aacgtctacc atataagaac
     7261 ctgagttttc aaatgaacat gaagagcatt ttgtattata tattctattt agagataact
     7321 gattgtgata gtatcccttt aagaaagcat atatagtgtc attaaactcg acaaacacaa
     7381 cggactcaat atatatatgg aatttatcat tggggtgcca ctctaattca agtcttcttg
     7441 tagagtcatc aaaggaaaag tttgaaataa aatcccagtt acctaaatct ataaaatcaa
     7501 cgatatttct acctgcacca tggatatttt tatttattaa ag
//
LOCUS       DAZKIF010000184         7538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZKIF010000184 DAZKIF010000000
VERSION     DAZKIF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.185"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1386
                     /locus_tag="ACHOTW_004301"
     CDS             <1..1386
                     /locus_tag="ACHOTW_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFP9846291.1"
                     /translation="RIAGISRDDGLITLQDAEGNTRLISPREAVAEGVTLYPPDTIRV
                     GTGDRMRFTKSDRGRGYVANSVWTVTAVSGDSVTLSGGQQTRVIRPAQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPA
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGRTEQA
                     VREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVR
                     DLQKEKTLGGD"
     gene            1406..2152
                     /gene="traX"
                     /locus_tag="ACHOTW_004302"
     CDS             1406..2152
                     /gene="traX"
                     /locus_tag="ACHOTW_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFP9846292.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            2211..3071
                     /locus_tag="ACHOTW_004303"
     CDS             2211..3071
                     /locus_tag="ACHOTW_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9846293.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            3174..3734
                     /locus_tag="ACHOTW_004304"
     CDS             3174..3734
                     /locus_tag="ACHOTW_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFP9846294.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            3867..4079
                     /locus_tag="ACHOTW_004305"
     CDS             3867..4079
                     /locus_tag="ACHOTW_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP9846295.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            4324..4785
                     /locus_tag="ACHOTW_004306"
     CDS             4324..4785
                     /locus_tag="ACHOTW_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP9846296.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            4831..5040
                     /gene="hha"
                     /locus_tag="ACHOTW_004307"
     CDS             4831..5040
                     /gene="hha"
                     /locus_tag="ACHOTW_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFP9846297.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            5078..5416
                     /locus_tag="ACHOTW_004308"
     CDS             5078..5416
                     /locus_tag="ACHOTW_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846298.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            5656..5910
                     /locus_tag="ACHOTW_004309"
     CDS             5656..5910
                     /locus_tag="ACHOTW_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP9846299.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            6146..6220
                     /gene="tap"
                     /locus_tag="ACHOTW_004310"
     CDS             6146..6220
                     /gene="tap"
                     /locus_tag="ACHOTW_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP9846300.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            6213..7070
                     /gene="repA"
                     /locus_tag="ACHOTW_004311"
     CDS             6213..7070
                     /gene="repA"
                     /locus_tag="ACHOTW_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP9846301.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT         1939 a         1754 c         2157 g         1688 t
ORIGIN      
        1 cggattgccg gtatcagcag ggatgacggg ctgataactc tgcaggatgc ggaaggtaac
       61 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacactgta ccccccggac
      121 accatcaggg tggggaccgg tgaccggatg cgcttcacga agagtgaccg ggggcgtggt
      181 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacgctgtcg
      241 ggcggacagc agacccgggt gattcgcccc gcccaggagc gggcagagca acatattgac
      301 ctggcctatg ccatcaccgc tcacggtgcg cagggggcaa gtgaaacctt tgccatcgcg
      361 cttgaaggta cggaaggcag ccggaaactg atggccggct ttgagtcagc ctacgtggcc
      421 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
      481 gccattaaca atgccgtaca gaaaggaaca gcccacgatg tgtttgaacc gaaaccggac
      541 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
      601 gcagggcgtg ccgttcttcg tcaggcgggg cttgccgggg gagacagtcc tgcacggttt
      661 attgctccgg gacgtaaata tccacagccg tatgtggcac tgccggcgtt tgaccgtaac
      721 ggcaagtctg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
      781 ggattcagtg gtgaaggacg ggtgaaaggc agcggggatg cgcagttcgt ggccctgcag
      841 ggcagccgta acggagagag cctgctggct gataatatgc aggatggtgt ccggattgcc
      901 cgtgataatc ctgacagcgg tgtggtggtg agaatcgccg gtgaaggtcg tccgtggaat
      961 cccggtgcca tcaccggtgg tcgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     1021 ggagccggaa atggcgagcc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     1081 gccatccgcc gtgaaacgga acgccgcgca gatgaaattg tccgtaaaat ggcagagaac
     1141 aaacctgacc tgccggacgg cagaacagag caggctgtca gggagattgc cgggcaggag
     1201 cgtgaacggg ctgtcacttc tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     1261 ccacaacggg agcgggaggc ggtccgtgag gttgtccggg aaaacctgct gcaggagcga
     1321 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cctgggcgga
     1381 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     1441 ttcactggct gcccggaccg atacctggtt gcagtcgttg ctggtctggt cacccggaca
     1501 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccacg caaaccgcat
     1561 tctgcatctg gaccagacgt ggatgtttct ggccgggcgt ggagcctttc cgttgtttgc
     1621 tctggtgtgg gggctgaatc tgtcccgcca tgcgcatatc aggcagtcgg ccattaaccg
     1681 gctgtgggga tggggaatta ttgcccagtt cgcgtattat ctggcaggct ttccgtggta
     1741 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     1801 gcgcagtggg tggcgtacag cagcggccat tctcctgatg gcactgtggg ggcctttgtc
     1861 cggcaccagt tacggcattg ccgggctgct gatgctggca gtcagccacc ggctgtaccg
     1921 ggcggaagac agaacggaac gtctggtgct ggttgcatgc ctgctggccg tgattccggc
     1981 acttaacctt gcctccagtg atgcggcggc tgtagctggt cttgtgatga cggtgctgac
     2041 cgttggtctg gtgtcctgtg cagggaaatc attaccccgt ttctggcccg gagatttttt
     2101 cccgacgttc tatggctgtc atctggctgt gctgggcatt ctggcgttgt gactggtatg
     2161 atactgacgc tgattctgct tattaataaa caacataaca caggatattt atgaaaatta
     2221 cggatcacaa actctctgaa ggtattgccc tgacatttcg tgttcctgaa ggaaatataa
     2281 aacatccttt gattatctta tgccatgggt tttgcggtat caggaatgtt ctgctccctt
     2341 gctttgcgaa tgccttcaca gaagcaggat tcgcaacgat tacttttgat tatcgaggat
     2401 tcggtgagag tgatggagaa cgagggcgtc tggttcctgc aatgcagacc gaagatatta
     2461 tttcagttat caattgggca gaaaagcagg aatgtattga taatcagcgt attgggttat
     2521 ggggaacatc gctgggaggg gggcatgtct tcagtgcagc tgcgcaggac caacgcgtaa
     2581 aatgtattgt cagccagctg gcttttgctg acggtgatgt tctggttacc ggagagatga
     2641 atgaatcgga gagagcgtct tttctatcca cgctgaataa aatggcagag aagaagaaaa
     2701 acaccgggaa agagatgttt gtgggggtta cacgggtact aagtgatgat gaatcgaagg
     2761 tattcttcga gaaaatcaaa gcccggcatc ccgaaatgga tataaaaata cctttcctta
     2821 cggtcatgga aacgttgcaa tataaaccgg cagagagtgc agccagcgtc cagtgcccag
     2881 ttttggttgt tattgccggc caggatagcg ttaatccacc ggaacaggga agagcattat
     2941 atgatgctgt tgcttctggt actaaggaat tatacgagga agctgatgca tgccattatg
     3001 atatttatga gggcgcgttt tttgaacgtg tagtagctgt gcagacacaa tggtttaaac
     3061 aatatcttta attgtcagta tttatacgtt aaaggtcgtt gaaattaact tcctgttacg
     3121 atgagccgtt tcaacggcct gcagggcata ttttcacgaa ggtcagagta agcatggcag
     3181 agcaaaaacg accggtactg acactgaagc ggaaaacgga aggagagacg cctgtccgca
     3241 gccggaaaac catcatcaat gtcaccacgc caccaaaatg gaaggtgaaa aagcagaagc
     3301 tggccgagaa agcagcccgg gaagcggagc tggcggcaaa aaaagcgcag gccagacagg
     3361 cgctgtccat ttatctgaac ctgcccacgc aggatgaggc cgtgaacacc ctgaaaccct
     3421 ggtggccggg attatttgac ggtgacacgc cccggcttct ggcctgcggt atccgggacg
     3481 tgttactgga agacgtggcg cagcggaata tcccgctctc gcataaaaag ctgcgcaggg
     3541 cgctgaaggc catcacccgt tcagaaagct atctgtgtgc catgaaagcc ggtgcctgcc
     3601 ggtatgacac ggaagggtat gtgacggagc atatttctca ggaggaggaa gcgtatgcgg
     3661 cagcgcgtct ggataaaatc cgccgccaga accggataaa ggcagaactt caggccgtgc
     3721 ttgatgagaa ataaaaaaag cctccctcag agagaggagg cagggaaata aggcttaaaa
     3781 ggaaataact tccgtacccg aaaggaagcc aattcagtag attatttaac accataatac
     3841 tgacgggtta aagaggagtg ataaagatga atggattcag aaacagttca cggaacggtc
     3901 aggtctggcg ttaccagcgt gccggaagcc gggccgttat tctggaagtc agtggacgct
     3961 ggatggaagc ggcagaagca tggcgacggg ctgccggtgt tgcaccccgg actgactggc
     4021 agcagtttgc ccgaaaaagg gctgaacact gccaccggcg ctgccgagga agggggtaac
     4081 gaaaaaagca gccttcatta ctccagggat tcagaaggct gaataatgca tgagatttgc
     4141 tttttttaat atccggtcgg gcagtatgac cggccagttc tgagtagcac agagtttttt
     4201 ccgtattaag tcgtgatttt atattttgtg attaatttca caaaataagg tgttattaag
     4261 tgtgtgctgc aatattcagg atgcctgaaa tacaggtgct atttatctga tacggagaat
     4321 aacatgagga aatatattcc actggtatta tttatctttt catggccggt attgtgtgca
     4381 gatattcatg gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca
     4441 cggaaggcgg tgcggatcag gctggtaaat attgatgcgc cggaaaagaa acaggattat
     4501 ggccggtggt cgacagacat gatgaaatca ctggttgcag gaaaaacggt taccgtcaca
     4561 tattttcagc gagatcgtta tggtcgtatg ctcgggcagg tttatgcacc agacggaatg
     4621 aatgttaatc agtttatggt ccgggccggg gccgcctggg tatatgagca gtacaacaca
     4681 gatcccgtgc tgcctgtcct tcagaacgaa gccagacaac aaaaacgtgg gctctggtca
     4741 gatgctgatc cggttccccc ctggatatgg agacaccgta aataaagcga tggcaatctt
     4801 atcattcatc tgttctcagc gggaataaaa atggaaaaaa caaaacagga atggctgtat
     4861 caattacggc gatgttcatc agtcaatacg ctggaaaaaa ttattcataa aaacagagac
     4921 agtttatcga ccagtgaacg tgaatcattt aattcagcag cagaccaccg gttagcagaa
     4981 ttaatcaccg gaaagttata tgacaggata ccaaaagaaa tatggaaata tgtaaggtaa
     5041 tagttcagca taattaatta ccaggagcag ataaacaatg aagctgatta tttttattct
     5101 cattgttctg attattgcag cgttacttat ccggattatc ctcagaagtg taaatcagca
     5161 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg accggtgatg cagagcgaat
     5221 actgtccggc ggtgaatatg atgccagcca tttacgacct gaacgcagac gccgggatat
     5281 tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc acgatgccag
     5341 aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgctc agcagtcacc
     5401 ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa
     5461 gcaagttttc cccgctcccg gcgtttccat aactgaaaac catactattt cacagtttaa
     5521 atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc ttcgtcacaa
     5581 ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc cctgtttaag
     5641 tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatc tggcgcaaaa
     5701 cgagcataca gaaaggggaa tccgctttct gatgcagaga aacaaagatt atcagtggcc
     5761 cgtaaaagag cttcgttcaa ggaagtaaaa gtatttcttg aaccaaagta taaggccatg
     5821 ctcatgcaaa tgtgtcatga agatggcctg actcaggctg aagttctgac cgcactgata
     5881 aaaagtgaag cgcaaaaacg atgtgtgtga tgatgggctt acattcttga gtgttcagaa
     5941 gattagtgct agattactga tcgtttaagg aattttgtgg ctggccacgc cgtaaggtgg
     6001 cagggaactg gttctgatgt ggatttacag gagccagaaa agcaaaaacc ccgataatct
     6061 tcagaaagtt tggcgactga gaagattacc ggggcccact taaaccgtat agccaacaat
     6121 tcagctatgc ggggagtata gttatatgct cggaaaagtt caagacttct ttctgtgctc
     6181 gctccttctg cgcattgtaa gtgcaggatg gtgtgactga tcttcaacaa acgtattacc
     6241 gccaggtaaa gaacccgaat ccggtgttca caccccgtga aggtgccgga acgctgaagt
     6301 tctgcgaaaa actgatggaa aaggcggtgg gcttcacctc ccgttttgat ttcgccattc
     6361 atgtggcaca tgcccgttcg aagggactgc gtcggcgcat gccaccggta ctgcgtcgcc
     6421 gggctattga tgcgctgctg caggggctgt gttttcacta tgacccgctg gccaaccgtg
     6481 tccagtgctc catcaccaca ctggccattg agtgcggact ggcgacggag tctgctgccg
     6541 gaaaactctc catcacccgt gccacccggg ccctgacgtt cctgtcagag ctgggactga
     6601 ttacctacca gacggaatat gacccgctta tcgggtgcta cattccgacc gacatcacgt
     6661 tcacaccggc tctgtttgct gcccttgatg tgtctgagga tgcagtggca gctgcgcgcc
     6721 gcagccgtgt tgaatgggaa aacaggctgc gcaaaaagca ggggctggat accctgggta
     6781 tggatgaact gatagcgaaa gcctggcgtt ttgtgcgtga gcgtttccgc agttaccaga
     6841 cagagcttaa gtcacgggga ataaagcgtg cccgtgcgcg tcgtgatgcg ggcagggaac
     6901 gtcaggatat cgtcaccctg gtgaaacggc agctgacgcg tgaaatctcg gaagggcgct
     6961 tcactgctaa tcgtgaggcg gtaaaacgcg aagtggagcg tcgtgtgaag gagcgcatga
     7021 ttctgtcacg taaccgcaat tacagccggc tggccacagc ttccccctga aagtgacctc
     7081 ctcagaataa tccggcacgc gccggaggca ttcgcacgcc tgaagcccgc cggtgcacaa
     7141 aaaatcagca ccacatccca aaaacaacct catcatctac cttctggtgc atccggtccc
     7201 ccctgttttg atacaaaaca cgtctcacag acgggcaatt ttgtttatcc acattaaact
     7261 gcaagggatt tctctgtaag attacaaccg ttcatgtcat aaagcaccag ccgccaggct
     7321 tacagggtgc aatgtatctt ttaaacaccc gtttatatct cctttaaact acttaattac
     7381 attcatttaa aaaaaagact tattcactgc ctgtcctgtg gacagacaga tatgcacctc
     7441 ccaccgcaag cggcgggccc caaccggagc cactttagtt acaacacaca aaaacaacct
     7501 ccagaaaaac cccggtcccg cgcagaactg aaaccaca
//
LOCUS       DAZKIF010000185         7452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZKIF010000185 DAZKIF010000000
VERSION     DAZKIF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.186"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..691)
                     /locus_tag="ACHOTW_004312"
     CDS             complement(<1..691)
                     /locus_tag="ACHOTW_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP9846302.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRR"
     gene            complement(702..1703)
                     /gene="ltaE"
                     /locus_tag="ACHOTW_004313"
     CDS             complement(702..1703)
                     /gene="ltaE"
                     /locus_tag="ACHOTW_004313"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP9846303.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1740..3458)
                     /gene="poxB"
                     /locus_tag="ACHOTW_004314"
     CDS             complement(1740..3458)
                     /gene="poxB"
                     /locus_tag="ACHOTW_004314"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP9846304.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3591..4559)
                     /gene="hcr"
                     /locus_tag="ACHOTW_004315"
     CDS             complement(3591..4559)
                     /gene="hcr"
                     /locus_tag="ACHOTW_004315"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP9846305.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4571..6223)
                     /gene="hcp"
                     /locus_tag="ACHOTW_004316"
                     /gene_synonym="priS"
     CDS             complement(4571..6223)
                     /gene="hcp"
                     /locus_tag="ACHOTW_004316"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP9846306.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6367..7266)
                     /gene="lysO"
                     /locus_tag="ACHOTW_004317"
     CDS             complement(6367..7266)
                     /gene="lysO"
                     /locus_tag="ACHOTW_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP9846307.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1793 a         2016 c         1969 g         1674 t
ORIGIN      
        1 agcggcgctt accgctcacc gccataaaat gttcaaactg ttgctgataa ctgagcacct
       61 ctggtccggc ggcttcgaag atgcggtgtt cgttggctgg atggtctaac agcgccacca
      121 gatagtgcag caagttttcc agcgcgatgg gcgtggtgcg tgaacgtacc cagcgtggcg
      181 gcgttaacac tggcaggttg tagaccatat cgcgcatgac ttcgaacgct gctgaacctg
      241 caccaacgat tattccggca cgcaattcgg tcactggtac attcgcttca cgaagaatgt
      301 ccgccgtagc ctgacgcgcg cgcagatggt ccgactgctc atgtggcggg gcctgcaacg
      361 aactgagaaa gattaattgc ttaactggta cttcacgtag cgcatcgcgg aagttgagag
      421 ccacctggcg ctcctgagcg ataaaatcgc cgccttcgcc catgctatgc accagaaaat
      481 agaccgtatc gatatcctgt aacagggccg gaaggttatc cggccagctg agatcgactt
      541 tatggcaact gacgtttgcc agttgcagct ttgcaagcct gtcgacatga cgtgccgccg
      601 ccaggatctg atgcccttgc tggctgagtg tgcgcaccag atgctgacca atgtagccac
      661 tggcaccaag aactaaaatg cgttgcggca cgtctccctc cttaacgcgc caggaatgcg
      721 cgccagtggg cggcgacttc tgccagttgt tcgcgcgaga cgtcaagatg ggtcaccagg
      781 cggacaatcg gcgaggcgtt aatcagtacg tttctcgctt tcatgtattc gcctaacgcg
      841 gcagcatttt cttcgccgac gcgaacaaac agcatattag tgtcctgacg catcacatcc
      901 gcgcctgctt cacgcagttg ctccgccatc caggcggcgt tgtcgtgatc ttcctgcaac
      961 cgtgcgacgt tatttttcag cgcatacatc ccggcggcag ccagaatgcc ggactggcgc
     1021 atcccgccac ctgtcatttt ccgccagcga atggcacgtt taatgtaatc acgattaccg
     1081 acgagtaatg aaccgactgg cgtcccaaga cctttcgaca ggcaaatggt gaacgaatca
     1141 caatattgcg tgagctcttt cagttcgctg ccgtaagcca ccacggcatt aaagatgcgc
     1201 gcaccgtcaa catgcagcgc cagattgcgc tcgcgggtaa attcccatgc atctttaagg
     1261 tattcacgcg gcaacacttt gccgttgtgg gtgttttcca gactgagtaa tttggtgcgg
     1321 gcgaaatgga tatcgtcggg tttgattttc atcgccactt tatccagcgg tagcgtgccg
     1381 tcggcagccg cgtcgatggg ttgcggttga atactgccca gcaccgccgc gccaccggct
     1441 tcaaacagat agttatgcgc ggcctgaccg acaatatact cttcgccgcg ttcgcagtga
     1501 ctgagcagag cgaccaggtt ggcctgagtg ccggtaggca gaaaaatggc ggcttcttta
     1561 ccggaaagct ctgctgcata gtcctgcaga gcattaacgg tagggtcgtc tccgtaaacg
     1621 tcgtccccaa ccggggcggc catcatcgct tcgagcatgg cgcggctcgg tcgggttacg
     1681 gtatcactgc gtaaatcaat catggcatgt ccttattatg acgggaaatg ccaccctttt
     1741 taccttagcc agttcgtttt cgccagttcg atcacttcat caccgcgtcc gctgatgatt
     1801 gcgcgcagca tatacaggct gaaacctttg gcctgttcga gtttgatctg cggtggaatg
     1861 gctaactctt ctttggcgac caccacatcc accaataccg gaccgtcgat ggagaaggcg
     1921 cgttgcaggg cttcatcgat ttcagaggct ttttctacac gaatacccgt aattccgcac
     1981 gcttcggcaa tgcgggcaaa gttggtgtcg tgcagctcag taccgtctgt caggtagcct
     2041 ccggctttca tctccatcgc cacaaagccc agcacgctgt tattaaagac gataattttc
     2101 accggcagtt tcatctgcac taccgagagg aaatcgccca tcaacatgct aaaaccgcca
     2161 tcgccgcaca tggcgaccac ctgacgttcc ggctcggttg cctgcgcgcc cagcgcctgc
     2221 ggcatggcgt tagccatcga accgtggtta aacgaaccta acaggcgacg cttgccgttc
     2281 attttcagat aacgcgccgc ccacaccgtt ggcgtgccga cgtcacaggt aaagatggcg
     2341 tcatcggcgg caaaatgact aatttgctgc gccagatatt gcgggtgaat ggctttctcg
     2401 ctcggtttag ctaaatcgtc cagccctttg cgggcatcgc ggtaatcttc cagcgcttta
     2461 tccagaaact tgcgatcggc tttttcttcc accaatggca gcaatgcacg cagggtcgac
     2521 ttgatatcgc cgaccagcgc catatccacc ttgctgtgtg cgccgatgct ggctgggttg
     2581 atatcaatct gaataatttt ggcatcggtc gggtagaacg cacggtaggg gaactgagta
     2641 ccgagtagca ctaacgtgtc ggcgttcatc atggtatgga aacctgacga gaatccgatt
     2701 aacccggtca ttccaacatc atacggatta tcgtattcga catgctcttt accgcgtagg
     2761 gcatgaacaa taggtgcttt aattttcccg gcaaactcaa ctaactcttt atgcgccccc
     2821 gcgcagccgc tgccacacat cagagcgata ttgctggaat aacgcagcag ttgcgccagt
     2881 ttgcgtaact cttcttcttc cggcgtcacg actggctgtg gcgcatgata ccagtgggta
     2941 gttgcccctt ctggcgcagg ttttaacgcc acgtcgcccg gtaacacgac aacggaaacg
     3001 ccacggttaa gcaccgcttt gcgcatggca atcgccagca cttgtgggat ctgctccggg
     3061 ctggaaacca gctcgcaata gtgactacat tcgcggaata gctcttgtgg gtgggtttcc
     3121 tggaaatagc cgctgccaat ttcgctggaa ggaatatgag cggcaatcgc cagtaccgga
     3181 acgtgattgc ggtggcaatc gaacaggccg ttgattaagt gcaggttgcc ggggccgcac
     3241 gatccagcac agaccgccag ttctcctgaa agttgtgctt cagcgccagc ggcaaacgcc
     3301 gccacttctt catggcgggt ggacatccac tcgatggtgc ccatgcgatt aagactgtcg
     3361 ctaagaccgt tcagagagtc gcccgtgact ccccagatgc gtttcacgcc tgccgattcg
     3421 agtgttttgg cgatataagc tgcaaccgtt tgtttcatgg ttctccatct cctgaatgtg
     3481 ataacggtaa caagtttagt tcatctgacg gagggggaag ggatgggaga gaaaggaggc
     3541 actaacggtt aaatagcccg atgaaaggaa tatcatcggg cataaggcga ttatgcgaga
     3601 accaaatccc cctgcggatg gcaggagcag gccagtacgt aaccttcagc gatttcggcg
     3661 tcggtcagcg tcattgtact gctcaccgta tattcaccgg aaaccacttt cgtcttacag
     3721 cagccgcaaa cacccgcgcg gcaggcggcg acaaccggaa cgttattgct ttccagcgcc
     3781 tccagcagcg tggtgccaac aggagcgtaa aattcttgcg ccggttgcag tttggtgaat
     3841 ttcagaccgc tggtcgcagc ttccgctact ggggtgaaga atttctcttt aaagaaacgc
     3901 gtcacgccga gtgctttcac ttcctgctct acccaatcca tatacggagc cgggccgcag
     3961 gtcatcacgg tacgtgaagc taagtcagga acgcgagtca ggagttcgcg agtgagacga
     4021 ccagcgataa agccttcggt aacgttattt tccgccacca gcgttaccgg atagttacgc
     4081 cactcatcgg cgaaaataac atcctgcggc gtacgcacgt tgtagatcac ccgcacatcg
     4141 gcctgtggac ggttcttcgc cagccagcga cgcatcgaca taatcggcgt gacgccgcag
     4201 cctgcagcca gcaacaggaa tttatcttct gctttatcgt cgcaggtaaa ttcccccatc
     4261 gcgtctgaaa gccagagata atcaccgcgt tttacatcgc gggtcagcca ctgggagccg
     4321 acaccgtcat caatccgccg cacggtcagg gtgatatatt cactcacgcc tggcgtggag
     4381 gaaatggtgt aagcacgcag cgtttccgct gagttacgca cgctgaccag tgcatattgc
     4441 ccggcgcggt atgggtagta atcgtggcaa atcagggaaa tcgtccacac atccggcgtt
     4501 tcttgcttaa tgtgatgaac ctgcatccgc cacgggcatt gattcgttgg catcgtcatc
     4561 gacaaactcc ttacgcgctc aacagttgct tcatgtcttc ttcaacagtg gtgatagaac
     4621 gcaggccgaa tttctcgttc agcaccgcca gcagatctgg tgtcaggaaa ccaggtgcag
     4681 tcgggccggt gacgatattt ttcacgccca gagaaagcag cgtcagcaga atgacaatcg
     4741 ctttctgttc aaaccaggag agcaccagcg acagcggcag atcgttgaca ccgcagccca
     4801 gtttttctgc cagcgtaacc gccagaataa ttgctgagta agcatcgtta cactgaccgg
     4861 catccaccag acgaggcaga ccttcgatat cgccaaactc aagtttgtta aagcgatatt
     4921 taccacaggc gagagtcagg atcaggcagt catctggcac gctggtggcg aaatcggtga
     4981 agtagtgacg ttcgccgcgt gcgccgtcac agccaccaag caggaagata tgacgcagtt
     5041 tttcacggct caccagatca attagcgtat ccgctgcgcc aagcagcgtc tggcggccaa
     5101 agcccacggt gatcaggtgc ggaatttcgc tgtatgggaa gcctgccatt tgttgcgcct
     5161 gggcaatgac cgcagagaaa tcttcgccat ccagatgacg cacgccaggc cagccaacga
     5221 tgctgcgggt ccagatacga tcgtcataag cgcctacggt tgggtcgatg atgcagttcg
     5281 aggtcatcac gatggggcca gggaaacgag cgaactccac ttgctgattc tgccagccgc
     5341 tgccgtagtt accgaccaga tgcttgaatt tacgcagctc cgggtagcca tgcgcaggca
     5401 gcatttcgcc gtgggtgtag acattaacgc ccgtgccttc ggtctgttcc agcaggttgt
     5461 agagatcttt gagatcgtga ccggaaatca ggatgcattt acccgccgtc gctttgacgt
     5521 tgacctgggt tggcgtcggg tgaccgtatt taccggtttc gcctgcatcc agaatgctca
     5581 tcactttgaa gttcatctgg ccgatttcca ttgaacactc aagaagcgcg ttcatatcgg
     5641 caggccaggt ccccagccac gccatgattt tatgatactg ggcataaata tcgttgtcgt
     5701 attgaccgag aacgtgtgcg tgttccatat aggccgccgc acctttcagg ccatacaggc
     5761 acagcagacg caggccgaga atgttttcgc caatcgccgc tttatctttg ttaggggtaa
     5821 attctgctgc ctgacgttgc agttcgccga gatcgtcgct caccagttgc aggtcagcca
     5881 tcgggttatc gacgcgggcg ttggcatcta cagccaggca ttgtgctttc agcgcctcgc
     5941 gcaaggcaat cgcttcacga gcgtagccga caatacgcgg agaatcgaag ttaacgttgg
     6001 tcagggttga gaaaaaggca cgtggcgcga agctgtcgac atcgtggttg atgatgccgt
     6061 attcacgcgc ttttaccgcc caggcagaaa gcccttgcag cgccgcgatg agtaaatcct
     6121 gaaggtcaga agtttccgcc gttttaccac acatcccctg cgcgtatgag cagccgtttc
     6181 ctgccggagt acggatagtt tgttcacatt gcacacaaaa catgatcaca ccttttaaag
     6241 ttatatttaa tatacatgtt taaggttaag acgcttaacg cggggataaa agggattttt
     6301 catgcaactt taagggagat tgatttagcg caattttggc ggcagggatc taccgccaga
     6361 gaggtattac gcggagaaaa aggcgatgag gatcggcact aacaggctaa gaataaaacc
     6421 gtgaacaatt gccgccggga ccatatccag cccgccagta cgttgaagaa cgggcagggt
     6481 gaaatccatc gacgtcgcac cgcataagcc cagtgcggta gagcggctgc ggcgaatcag
     6541 cccagggatc aacataattg caatcagctc acgggccaga tcattaaaaa acgccgcgct
     6601 cccgattacc ggaccaaaag attcggtcaa taaaataccg gaaagagaat accagccgaa
     6661 accagaggcc attgccagcg cggtattgat gggtagatca agaataaagg cgttaattaa
     6721 accaccaatt aatgaactgg caaccaccac cacggcgaca atcattcccc ggcgattaag
     6781 gacaatctgc tttaaggtca tgccattatt gcgcaactga ataccgacga ggaaaagtag
     6841 cagaattaac gtgtattcac tggcttcggt cgcgtgttgt aagaaagcca gtccactcag
     6901 gccaatggca aaaccaatca ctactacgcc gcacagtttt agcgactcaa gcgccatcgc
     6961 aatacgcgac gggagttttt cttgctgatg gtggttgcgc cacggcagac cgcgctccag
     7021 ccacataagg gcggcaatat tacacagtaa aataacggta atactgacgg cagaataatg
     7081 cagaatcgcc aacaggttac tggcgaggtt atcgagaaac gccagactga tacccataaa
     7141 aaagagaata aggtaaacca tccagcttaa tagctgatta ataactttta acgcagcttg
     7201 ttggcgaagc ggaatgaggt aacccacaat caggggaacc agaatgatta acaacccaga
     7261 aaacatgaaa aaccagccct tgcaaagaaa gaagtcgaaa tgcgcgatga cacactactg
     7321 aaagcggaag gacgagtaaa gttgcaatta aaaggaaatg ttatgcatca gagggggatt
     7381 tttaattcta tatgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
     7441 gccactggcg ca
//
LOCUS       DAZKIF010000186         7311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZKIF010000186 DAZKIF010000000
VERSION     DAZKIF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.187"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            104..1732
                     /locus_tag="ACHOTW_004318"
                     /pseudo
     CDS             104..1732
                     /locus_tag="ACHOTW_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
     gene            complement(1732..1854)
                     /locus_tag="ACHOTW_004319"
                     /pseudo
     CDS             complement(1732..1854)
                     /locus_tag="ACHOTW_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2082..3152
                     /gene="dhaK"
                     /locus_tag="ACHOTW_004320"
     CDS             2082..3152
                     /gene="dhaK"
                     /locus_tag="ACHOTW_004320"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9846308.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     GVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            3163..3795
                     /gene="dhaL"
                     /locus_tag="ACHOTW_004321"
     CDS             3163..3795
                     /gene="dhaL"
                     /locus_tag="ACHOTW_004321"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9846309.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSCVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAEFAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            3806..5224
                     /gene="dhaM"
                     /locus_tag="ACHOTW_004322"
     CDS             3806..5224
                     /gene="dhaM"
                     /locus_tag="ACHOTW_004322"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9846310.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSDTEISDSCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSPL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSPVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            5544..7228
                     /gene="treA"
                     /locus_tag="ACHOTW_004323"
                     /pseudo
     CDS             5544..7228
                     /gene="treA"
                     /locus_tag="ACHOTW_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT         1929 a         1822 c         1843 g         1717 t
ORIGIN      
        1 ttgcaccata caaatatcat gatgataaga ggatattatt cattgactta aacaaaatga
       61 caaaatccat agacccatag cctgggtact atataggagt ggtatgggca tcaaacaaca
      121 caatgggaat accaaagccg atcgtctcgc tgaattaaaa atccgttcgc cctcaattca
      181 actgataaaa tttggcgcta ttggtttgaa tgcaattctc ttttcccccc tgctgatagc
      241 tgctgataca ggaagtcaat atggcaccaa tattactatt aatgatggtg acagaattac
      301 tggagatacc gccgatccat caggaaacct ctatggtgta atgaccccag caggaaacac
      361 gcctggcaat atcaacctgg gtaatgatgt caccgtcaat gtcaacgacg cctctggata
      421 tgcaaaagga atcattattc agggcaaaaa cagctccctg acagctaacc gactcacagt
      481 agatgttgtt ggtcaaacct ctgccatcgg cattaattta attggtgact atacccatgc
      541 tgacttaggc acaggcagca ccattaagag taacgatgac ggcatcatta ttgggcatag
      601 ctcaacacta acagccactc aattcaccat tgaaaactcg aacggtatag gcctaaccat
      661 caatgactat ggcaccagtg tcgatcttgg aagcggaagt aaaatcaaga ccgatggaag
      721 tacaggtgtt tatatcggtg gtctcaacgg caataacgcc aatggtgctg cgcgttttac
      781 ggcgacagac ctgacaatcg atgttcaggg ctacagcgcc atggggataa acgtacagaa
      841 aaactctgtt gtcgatctcg gaacaaacag ttccattaaa accagtggcg ataatgcaca
      901 cggcctctgg agctttggcc aggttagcgc gaatgcactc actgttgatg taactggagc
      961 cgcggccaat ggcgtcgaag ttcgtggtgg tacaaccact atcggtgcag atagccatat
     1021 ttcttccgcg cagggcggtg gtctcgtcac cagtggttca gacgcgacaa tcaatttttc
     1081 tggcacggca gcgcaacgaa acagcatctt ttccggcggt tcttatggtg cctcggccca
     1141 gacggcaacg gctgttatca acatgcaaaa taccgatatt acggttgatc gtaatggcag
     1201 tctggcgctg ggtttgtggg cgctcagcgg cggtagaata accggagaca gtttggctat
     1261 caccggcgcg gcaggagcca gggggattta tgccatgacc aacagccaga tcgacctcac
     1321 gagcgatctg gtcattgata tgagtacacc cgaccagatg gccatcgcaa cgcaacatga
     1381 cgatggttat gccgccagcc gcatcaacgc ctcgggtcgt atgcttatca acggtagcgt
     1441 tctttccaaa ggtgggctaa tcaatctgga tatgcaccct gggtcggttt ggacaggttc
     1501 ctccctcagc gataatgtca atggcgggaa actggacgtt gcaatgaata acagcgtctg
     1561 gaacgtaaca agtaattcta atctcgacac gctggcgctg agccattcaa ctgtcgattt
     1621 tgccagccac gggtcaactg ccggcacatt taccacatta aacgtagaga acctgagcgg
     1681 taacagtacc tttattatgc gtgctgatgt tgttggcgag ggtaatggcg ttaagccctg
     1741 ggcctgatgt ggtacgttcc atgtctcccg tttcatcagc ttattgcatc gctcccagga
     1801 ggttgcaatt aagggagata tgccccgacc atcgttgtta aaagcgccac tcatatcccg
     1861 catcccttat gacaaaaggt gttccggctc gcaattttaa gtattaatac acgtaaaagt
     1921 ttcaatatgg aacattcatt aaagcatatg tttcactatg aaacataatt tgggctaatt
     1981 ttcttttccc ttgcctgatg cacaatggat tcatcggcag tcagtggtcg ccgtgtcgtt
     2041 gaacatcatc catgccctac cgtaattgct ggagcaaaat aatgaaaaaa ttgatcaatg
     2101 atgtgcaaga cgtactggac gaacaactgg caggactggc gaaagcgcat ccatcgctga
     2161 cactgcatca ggatccggtg tatgtcaccc gagctgatgc ccctgttgca ggaaaagtcg
     2221 ccctgctgtc gggtggcggc agcggacacg agccgatgca ctgtggttat atcggtcagg
     2281 ggatgctttc gggggcctgt ccgggcgaaa ttttcacctc accgacgccc gataaaatct
     2341 ttgaatgcgc catgcaagtt gatggcggcg aaggtgtact gttgattatc aaaaattaca
     2401 ccggcgatat tcttaacttt gaaacagcga ccgagttact gcacgatagc ggcgtaaaag
     2461 tgaccactgt ggtcattgat gacgacgttg cagtaaaaga cagtctttat accgccgggc
     2521 ggcgcggcgt tgccaacacc gtattaattg aaaaactcgt aggcgcagcg gcggagcgtg
     2581 gcgactcact ggacgcctgt gcggaactgg ggcgtaagtt gaataatcaa ggccactcaa
     2641 taggtatcgc tctcggtgcc tgtaccgttc ctgccgcggg caaaccttct tttaccctgg
     2701 cggataatga gatggagttt ggcgtcggca ttcatggtga gccgggtatt gaccgtcgcc
     2761 ccttctcttc ccttgatcaa accgtcgatg aaatgttcga caccctgctg gaaaatggct
     2821 cataccatcg cactttgcgt ttctgggatt atcaacaagg cagttggcag gaagaaccac
     2881 aaaccaaaca accgctccag tctggcgacg gggtgattgc gctggttaac aatcttggcg
     2941 caactccact ttctgagctg tacggcgtct ataaccgcct gaccacacgt tgccagcaag
     3001 cgggattgac tatcgagcgt aatttaattg gcgcgtactg cacctcactg gatatgaccg
     3061 gtttctcaat cactttactg aaagttgatg acgaaacgct ggcactctgg gacgccccgg
     3121 tccacacccc ggcccttaac tggggtaaat aaggagaaag caatgtcact gagcagaact
     3181 caaattgtta actggctcac tcgttgtggc gatattttca acaccgagag cgagtatctt
     3241 accggactgg atcgcgagat tggcgacgct gaccacgggc taaatatgaa ccgaggcttt
     3301 agcaaagtgg tggaaaaact ccctgctatt gcagataaag atatcggttt cattctcaag
     3361 aataccggta tgacgctgct ttcctgcgtc ggtggtgcca gtggtccgct gttcggtacc
     3421 ttctttatcc gcgccgcaca ggcgacccag gcacggcaaa gcctgacgct ggaagagctt
     3481 tatcagatgt tccgcgatgg cgcggacggc gtaatcagtc gcgggaaagc cgaacctggc
     3541 gataaaacca tgtgtgatgt gtgggtgccg gtggtggaat cgttacgtca gtccagcgag
     3601 caaaatctct ctgttccggc ggcgctcgaa gctgccagta gcatcgccga attcgctgca
     3661 caaagtacga ttacgatgca agcccgcaaa ggccgcgcca gttatctcgg tgaacgcagt
     3721 attggtcacc aggatcccgg cgcaacctcg gtgatgttta tgatgcaaat gttggcgtta
     3781 gccgcaaaag agtaaggaat tggtgatggt aaacctggtc atagtttcac atagcagccg
     3841 actgggagaa ggtgtcggtg aattagcccg tcagatgtta atgagtgata attgtaaaat
     3901 cgccattgct gcgggaattg acgatccaca aaatcccatt ggtaccgatg ccgtcaaagt
     3961 gatggaggcc atcgaatctg ttgctgatgc cgaccatgtg ctggtcatga tggatatggg
     4021 tagcgcatta ttgagtgctg aaactgcgct ggaattgctg gctcccgaga tcgccgcaaa
     4081 agtacgtttg tgtgctgcgc cgttggtcga aggtacactg gcagcaactg tcagcgcggc
     4141 ctcgggggcg gagatcgaca aagttatctt tgacgccatg catgcgctgg aagccaaacg
     4201 tgaacaactg ggtttaccgt cctccgacac tgaaatctct gactcatgtc ctgcgtacga
     4261 tgaagaagcc cgttctctgg cggtggtcat taaaaaccgt aacggcctgc acgtccgtcc
     4321 ggcctcccgg ctggtttata ccttatcgac atttaatgcc gatatgttgc tggaaaaaaa
     4381 cggcaaatgc gtcacaccgg agagtattaa ccagattgcg ttactacaag ttcgctataa
     4441 cgatacgctg cgcctgattg cgaaagggcc agaagctgaa gaggcactga tcgctttccg
     4501 tcagctggct gaagataact ttggtgaaac ggaggaagtt gctccaccta ctctgcgtcc
     4561 cgttccgcct gtttcgggta aagcctttta ttatcaacca gttttatgta cggtacaggc
     4621 aaaatcaccc ctgaccgtgg aagaagaaca ggaacgatta cgccaggcca ttgacttcac
     4681 gttattagat ctgatgacgt taacagcaaa agcagaagcc agcgggcttg acgatattgc
     4741 cgcaatcttt tctggtcacc atacactgtt agatgatccg gaactgctgg cggcggcaag
     4801 cgaactcctt cagcacgaac attgcacggc agaatatgcc tggcagcaag ttcttaaaga
     4861 acttagccag caataccagc aactggatga tgaatatcta caagctcgct atattgatgt
     4921 ggacgatctt ctgcatcgca ccctggtcca cctgacccaa acgaaagaag aactcccgca
     4981 gtttaactcg ccaactattc tactggcgga gaacatttat ccctccccag tactgcaact
     5041 ggatccggcg gttgtaaaag gtatctgcct tagcgccgga agtccggtat cccacagcgc
     5101 cctgatcgcc cgtgaactgg ggattggctg gatttgccag cagggtgaga aactgtatgc
     5161 gatacaacca gaagaaaagc taacgctgga cgttaaaacg caacgtttca accgtcaggg
     5221 ttaagccacc gttcggatgg catcgttctg atgtcatccg gcaatttact cagcactaat
     5281 gttttttaac tacctgttat atttttcacc aatttatgat gatttttctt cgctaatcgc
     5341 gaaccaaacg acgctggctg gctaataaac agacgttatt tgatcactct atttcgccgt
     5401 tatttatccc tttaattccc ttttctaaaa tgcctgacag ttcgcagaat gagatttcga
     5461 tcatgcagct agtgcgatcc tgaactaagg ttttctgata cttgaatacc gttttattcg
     5521 gtttcgccta aggagaatga ttgatgaaat cccccgcacc ttctcgcccg caaaaaatgg
     5581 cgttaattcc agcctgcatc tttttgtgtt tcgctgcgct atcggtgcag gcagaagaaa
     5641 caccggtaac gccacagccg cctgatattt tattagggcc gctgtttaat gatgtgcaaa
     5701 acgccaaact ttttccggac caaaaaacct ttgccgatgc cgtgccgaac agcgatccgc
     5761 tgatgatcgg atgcagcaaa accagagcgg atttgatctg cgccatttcg ttaacgtcaa
     5821 tttcaccctg ccgaaagagg gcgagaaata tgttccgcca gagggacagt cactgcgcga
     5881 acatattgac ggactttggc cggtattaac gcgttctacc gaaaacaccg aaaaatggga
     5941 ttctctgtta ccgctgccgg aaccttatgt cgtgccaggc ggacgttttc gcgaggtata
     6001 ttactgggac agttacttca ccatgttagg acttgccgaa agcggtcact gggataaagt
     6061 cgcggatatg gtggccaatt ttgctcatga aatagacact tacggtcata ttcccaacgg
     6121 caaccgcagt tactatttaa gccgctcgca gccgcccttc tttgccctga tggtagagtt
     6181 actggcgcag catgaaggcg atgccgcatt gaagcaatac ctgccgcaaa tgcaaaaaga
     6241 atatgcttac tggatggacg gtgttgaaaa cctgcaagcc ggacaacagg aaaaacgcgt
     6301 tgtaaaactt caggatggta cccttctcaa ccgctactgg gacgatcgcg atacgccacg
     6361 accagagtca tgggtggaag atattgccac cgctaaaagc aatccgaatc gacctgccac
     6421 tgaaatttac cgcgacctgc gctctgctgc tgcgtctggc tgggatttca gctcgcgctg
     6481 gatggacaat ccgcagcaac taaatacctt acgcaccacc agcatcgtac cggtcgatct
     6541 gaacagcctg atgttcaaaa tggaaaaaat ccttgcccgc gccagcaaag ctgccggaga
     6601 taacgcgatg gcaaaccagt acgaaacact ggcaaatgcc cgtcaaaaag ggatcgaaaa
     6661 atacctgtgg aacgatcaac aaggctggta tgccgattac gacctgaaaa gtcataaagt
     6721 gcgcaatcag ttaaccgcgg ccgccctgtt cccactgtac gtcaatgcgg cagcgaaaga
     6781 tcgcgccaac aaaatggcga cggcgacgaa aactcatctg ctgcaacccg gcggcctgaa
     6841 caccacgtcg gtgaaaagtg ggcaacaatg ggatgcgcca aatggctggg caccgttaca
     6901 gtgggtcgcg acagaaggat tacaaaacta cgggcaaaaa gaggtggcga tggacattag
     6961 ctggcacttc ctgaccaatg ttcagcacac ctatgaccgg gagaaaaagc tggtggaaaa
     7021 atatgatgtc agcaccaccg gaacgggggg cggcggtggc gaatatccat tacaggatgg
     7081 ctttggctgg accaatggcg tgacgctgaa aatgctggat ttgatctgcc cgaaagagca
     7141 accgtgtgac aatgttccgg cgacgcgtcc actcagtgaa tcaacaacac agccggtaaa
     7201 acaaaaagag gcggaaccta cgccttaact ttgctgtata tcgagcaatg ccattatgtg
     7261 cataaaattg gcattgctct tttttcgcca cgaaatgccc cccattctac g
//
LOCUS       DAZKIF010000187         7265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZKIF010000187 DAZKIF010000000
VERSION     DAZKIF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.188"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..990
                     /gene="yiaU"
                     /locus_tag="ACHOTW_004324"
     CDS             16..990
                     /gene="yiaU"
                     /locus_tag="ACHOTW_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9846311.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(987..2123)
                     /gene="yiaV"
                     /locus_tag="ACHOTW_004325"
     CDS             complement(987..2123)
                     /gene="yiaV"
                     /locus_tag="ACHOTW_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9846312.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWGHYLYLDH"
     gene            complement(2129..2452)
                     /gene="yiaW"
                     /locus_tag="ACHOTW_004326"
     CDS             complement(2129..2452)
                     /gene="yiaW"
                     /locus_tag="ACHOTW_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9846313.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2635..2778
                     /locus_tag="ACHOTW_004327"
     CDS             2635..2778
                     /locus_tag="ACHOTW_004327"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846314.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            complement(2997..4535)
                     /gene="aldB"
                     /locus_tag="ACHOTW_004328"
     CDS             complement(2997..4535)
                     /gene="aldB"
                     /locus_tag="ACHOTW_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP9846315.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4643..5938)
                     /locus_tag="ACHOTW_004329"
     CDS             complement(4643..5938)
                     /locus_tag="ACHOTW_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFP9846316.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(6068..7219)
                     /gene="yiaY"
                     /locus_tag="ACHOTW_004330"
     CDS             complement(6068..7219)
                     /gene="yiaY"
                     /locus_tag="ACHOTW_004330"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP9846317.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1823 a         1891 c         1734 g         1817 t
ORIGIN      
        1 aattgggctg ttgcgatgac aaaattacaa cttaagtacc gggagttaaa aattatctcg
       61 gtaatcgctg ccagtgaaaa tatcagccat gccgcgactg tacttggcat cgcacaggcc
      121 aacgtcagca aatatcttgc tgattttgaa tcaaaagtgg gtttaaaagt ctttgaccga
      181 accacccgac aactgatgct cacacttttt ggcaccgcgc tattaccgta catcaacgac
      241 atgctggaca gaaacgagca gcttaataat tttattgcag attataagca tgagaaacgt
      301 ggtcgggtca ccatctacgc cccaaccggc ataattacct atttatccaa acatgtaatc
      361 gataaaatta aagatatcgg tgatatcacc ttatcgctaa aaacctgcaa tcttgagcgt
      421 aatgcctttt atgaaggcgt cgaatttcct gatgattgtg atgttttaat cagctatgcc
      481 ccaccaaaag atgaatcgct ggttgccagt tttattaccc aatatgccgt aaccgcttat
      541 gccagccagc gctatcttga gaaacatcct attagccgtc ctgacgaact cgaacatcac
      601 tcctgtattt tgatcgactc gatgatgatc gacgacgcga atatctggcg cttcaacgtc
      661 gccgggagta aagaggtgcg ggattatcgc gtgaagggaa attacgtttg cgataacacg
      721 caatcggcgc tggagctggc acgaaatcac ctggggattg tgtttgcgcc agacaaaagt
      781 gtgcagagcg atctgcaaga cggcacgctg gtgccctgct ttcagcaccc gtatgaatgg
      841 tggctggatc tggtggctat cttccgtaaa cgggaatacc agccctggcg ggtccagtat
      901 gtgctggatg aaatgctgcg tgaaattcgc caccagcttg ctcagtcgca gcaactgcgg
      961 cctgaacaag cggcggaaag cgaagattaa tgatcgagat aaaggtaatg cccccagctg
     1021 gtcatgcgta ataacacttt gcgcatcacc gagacatggc tgaaatgatc agagtagacc
     1081 gccacctgag cgtaaatacc gtctggtaaa gcgctcaaat cagtgtgctc attcagttca
     1141 atggtcgcga taacgccatc tgaacccggc gctgtgttta acgtctgtaa ggtgccggtc
     1201 gactgataag ctccgccggg aacagctgga ctaatggctg ccagcttacc gctgaacact
     1261 ttgcctggca gagcgttaaa taccacttcc gcatcatcgc cgggagccag gcgcagcaag
     1321 gagttctgac ggaactgcgc cacgatttgt cgtttctgat cgggtataaa caccatcacc
     1381 ggacgtagcg gcagcgacgc ggcataggta cccggacgaa tcagcacttg ggtcacataa
     1441 ccatcgctcg gcgcacgcac tatcgtctgc tcaaggttat attttgcttc cgcgagctgc
     1501 gctttcaggc tggcgatttg agaatgttca cccaacacca ggctatccag ctggctctgg
     1561 atctgttttt gttccgccgc cgatgacttt acggaggctt cctgcgccag ataattttgc
     1621 cgtgccacat caatatcgcg ttctgaaaac gggtttactt tcgcctggct gccgcgtgcg
     1681 taacgctggt actccttagc gaatttatcc cgcgtggcct ttgccttctg agtattcgcc
     1741 gccatctcat ctaactctgc gcccaacgcc cgctgtttat gttctgcggt aacgatatcc
     1801 gccatcagcc gatccacccg cgcctgataa cgcgtcgggt ccagtcgaaa tagcacctca
     1861 ccttttttaa tcagcgtatt tttcttatcc gtcacttcta tcaccacgcc tgtcacctgt
     1921 gggacaacag gaatagaaat aaccgctttt tgcgctttaa aggtatacgg atggttatag
     1981 ttcattaaca gaattagacc gctgacaata aatattccac ccagggccgc tgtgggaatg
     2041 gtccatttat ttacgggaat tttgaagatc ttaaacattg cccatgcaaa agccacatag
     2101 gtcaaaataa tcagtaaatc cataagtatt actcggcaga agaggatttg atgtcggcga
     2161 gctgtttctc cagcccggca atgcgctgtt gcagttgcat aacggatgaa tcatggcttt
     2221 gcattcccca tccccgctcc gggcggtaaa gcgtggccca aatccacaaa aatggccaga
     2281 taacatgcag cgtaaaaaga ctcacccaac cagcaacatg aatggcgtcg gcatgaggat
     2341 gattacgttt tttagcaatc aggtagggaa tatcatgtaa aataataatc ccatagaata
     2401 tcaccagaaa tacaaaaata agcactccca gtgcaaaata gtccaggaac atatttccct
     2461 cagagaatat aaaaaagaac aattaacgca tattatgaaa tgccatgatg caatgcaatt
     2521 tccttattta tataacgaca tattgttttt tatatacttt ctgaagtgtg ctgcgtggct
     2581 ttttaacagg tcgaggctta tcatattgaa ataatggaac tatcagccaa actaatgaat
     2641 aacttcagta aaacatccac aatgcagatt aagcgtatta aaccttcaat aatctaccgt
     2701 ttattgctga ttgtcctcgg aataccaatg gtgatttacg gtctggtttg cccgctcacc
     2761 atcgaaacgc gagattaaac tcctggcaaa acacgcccca gaaacgcact tttgttggcg
     2821 tagtctgggt tattgcacac gtaggtttaa aacgtaaatt gtaacaacgt gcgctttgtt
     2881 tatgccggat gcggcgtgta cgccttatcc ggcctacggt caggtcccca taggcatgat
     2941 aagacgcgta agcgtcgcat caggcaatta atacccgatg cgaccagctt cttacatcag
     3001 aacagcccca acggtttatc tgagtagctc actagcaggc acttggtttg ctggtaatgc
     3061 tccagcatca tcttgtgggt ttcgcgaccg atacctgatt gtttgtagcc accaaacgcc
     3121 gcatgtgccg ggtaagcatg ataacagttg gtccacacgc gcccagcctg tatgccgcgc
     3181 cccatcttat aggccagatt accgttgcgg ctccagacgc ccgcgcccag gccatattgc
     3241 gtatcgttcg ccagctccag cgcttcttcc atcgttttga aggtagtcac cgccagcacc
     3301 gggccaaaaa tctcctcctg gaacacgcgc atattgttct gaccaaacag aatcgtcggt
     3361 tcgaggtagt agccgtcttt cagttcacct tccagcagct tgcgccgccc gccggtgagc
     3421 acatcagcgc cctctttttt accgatatca atgtagttga ggatggtttc cagttgcccg
     3481 tgagaaacct gcgcacccat ttgcgtcacg ctgtcgagtg ggttaccgct gcgaatgctt
     3541 tcgacacggc ggatggcgcg ttccataaag cgttcgtaga ttgactcctg cactaaggca
     3601 cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa acagtgcaaa gccttccagc
     3661 gctttatcga aaaaggcatc ttcttcatcc atcacatcag caaagaagat atttggcgac
     3721 ttaccgccca gctccagcgt caccggaata atgttttgcg tggcgtattg cataatttgt
     3781 tgacccactt ccgttgagcc ggtaaacgcc actttggcga tgcgtttcga ggtcgccaga
     3841 tattcgccaa tttccccacc tgcaccgttg accacgttca ccacgcccgg cggcagtaaa
     3901 tcaccgacga tttccattag cagcagtaca gaaagcgggg taagacgtgc gggtttcagc
     3961 accacacagt tgcccgccgc cagcgcggga gccattttcc agctcgccat cagcagcggg
     4021 aagttccacg ggataatctg ccccaccacg cctaacggtt catggaaatg ataggccacg
     4081 gtttcgctat caacttcact gatcccacct tcctgcgccc gaatacacga ggcgaaatag
     4141 cggaaatggt caatcgccag cggtacatcc gcagcactgg tttcgcgaat gggtttgccg
     4201 ttatcccagg cttcagctgt cgctaacagc tcgaggtttt gttccattcg atcggcaatc
     4261 ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg cccatttatc tttcactttg
     4321 tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg aagacgccac ttcgcacagc
     4381 agctgcccgg tcaccggcgt caggttctgg taatactcac catcggcagg ggctacccat
     4441 tcgccgccaa taaagttgtc atagcggttt tttaacttga gggggaaacc atactcgccg
     4501 ggcttaatct gtgctgaagg gggattattg gtcatgatcg tctccttgcg gtgtgaggta
     4561 taacaagggt agacgtggct ggcgaaatct tcgccagtcg gtaacagctt ttcgacggct
     4621 atcacgaatt tacgggcaag ggttacagcg gataatgcgc gaagtacacg gcttgctggt
     4681 cattaatttt cttctcttgc gcaaaccctt ctttaacaat tgtccgtaaa tcagctcgaa
     4741 ccgtattatc agagacgccc aggctcgcgc taatttcagc aactgtgtac tctttccccg
     4801 gattggcgag catcacattc aggagcgtta cctggcgcgg attcaggcgt ttaagcacac
     4861 cagagctaaa cagcacacta tcgagttttg cgcttcgttg tgtaatttca tcgatatgct
     4921 gctgccagtt ttgcagagca cgtttaatga cacccgcctg atattcaagg aaataagtca
     4981 gatccatacc atctgattca gtgtactggt agctggcggc atatttgacc ggtgcagcat
     5041 gaagcaaacg gctgatggaa atatatttaa agacatcgta gcccgatttc agcatatacc
     5101 agtaaaacag cgcccgactg gtgcgcccgt tgccatcccg aaacggatgt tcatgggcca
     5161 gcataaaatg cagaatgcag gcacggacca acggatgaat atacccttca tggctgttta
     5221 accactggca aactttttcc agccgttccg gtaacaatgc cgcagcgggc ggttgatgca
     5281 ctatattgcc atcatagtcg gcgatcacaa catcatctgt ttcacgaaat tctcccggac
     5341 gatatttcgc gtcattgatt ccacccatac caatggcatg taactggcga ataagcgcag
     5401 gtgtcagcgg ttctgcgagt aagtgcggaa tttccgccat cagccgggca tttcccgcaa
     5461 tcatatgctc gtcttcagtg cgtggcgacc gtcctgtttc cagcattttt ctcgccacca
     5521 gcgttgtagt attcgcacct tccagctggg agctggtgat cggctcttcc aggcgtaact
     5581 ggcttaactc ggctccggcc ccgcgtaacc gttcagtctg atctttcagc gccagcgcgg
     5641 tagcacgttt gtcgacaagc tcgcacgctt ctgcaatcac cggggtaatg ttgaatccgg
     5701 cctgttcacc cgcttcattg caataattaa ttcgttggat cgccgcgttg cgcgccagac
     5761 gggtaagcgt ccacgccaca tcgacgtttt ctcctttttt gatgcggtac tgcaactcat
     5821 caaaaggcag ataacgtcct ttttcatcgg taacccggtt aaagcgcagg tgggcctgga
     5881 gttcctcacc cgataagtgt gataaaggaa tcagcgtggg aggtgatttg atagccattt
     5941 gcttatctca aaaaagcgca atattgagat agtgtatagc aggaaagaga aactatctca
     6001 aaaaaagcaa gttttgagat agttgaaaaa atggctgaca atttgccagc cgttgtggaa
     6061 atgatgatta catcgctgcg cgataaatcg ccacaatttc ttcgtgagtt gcctggatcg
     6121 ggttagtaaa gccacaggca tctttcaggg cattagccgc cagaaccgcg aaatcttctt
     6181 ctttcacgtt caggtcgcgt aggcctgccg ggatatccac tttcttcgcc agttcacgga
     6241 tggcgttaat gcaggcttca gcaccttccg cgtcgttttt acctgtcacg ttcacgccca
     6301 ttgcagcggc acagtcacgc agacgtgcag cggcgacttt gctgttgaat acctgaacat
     6361 gcggcagcag aacggcgtta catacaccgt gtggcaggtt gtagaaaccg cccagctggt
     6421 gcgccatcgc atgaacataa cccagagaag cattattgaa cgccataccg gcgaggaact
     6481 gggcataagc cattgcttca cgcgctttcg cattactgcc atcttcaacg gctaacggca
     6541 ggttttcggc aatcatggtc acggctttca gtgcacaagc gtcagtgatc ggcgtggcgg
     6601 caatagaaac atacgcttcg atagcgtgcg ttaaggcatc catacccgtt gcggcggtca
     6661 gtgacttcgg cataccaatc atcagagagg agtcattgac agaaagcagc ggagtgacat
     6721 gtttatcaac aatcgccatt ttgatatgac gcgcttcgtc agtgatgatg cagaaacggg
     6781 tcatttcaga ggccgtaccc gccgtggtat tgatggcgat catcggcagc tgcggttttg
     6841 cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc attggctgcc accagcgcaa
     6901 taccttttgc gcagtcgtgt ggagaaccac cgcctaagga gatcacgcta tcgcaattat
     6961 tctctttaag taatttcaaa cctgcggcga cgttttccgt ggtggggtta ggttgggtgc
     7021 catcataaat aacgctaaaa atattgcgtt cttccagtgc tttttgcaca tcgcccgcca
     7081 tacctaattt cgttaacata ctgtcagtga caattaaggt acgggtaaat ccataatctg
     7141 ccatcatatt cattgcatca gtcaatgaat cagcgccgat gacattcaca gaaggaataa
     7201 agaaagttga agatgccata acacactcct gaaagtgtaa aaaagggtgg gcagcttact
     7261 gagga
//
LOCUS       DAZKIF010000188         7245 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZKIF010000188 DAZKIF010000000
VERSION     DAZKIF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.189"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            69..1402
                     /gene="adeQ"
                     /locus_tag="ACHOTW_004331"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             69..1402
                     /gene="adeQ"
                     /locus_tag="ACHOTW_004331"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            1455..1907
                     /gene="yicN"
                     /locus_tag="ACHOTW_004332"
     CDS             1455..1907
                     /gene="yicN"
                     /locus_tag="ACHOTW_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP9846318.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2023..2346)
                     /locus_tag="ACHOTW_004333"
     CDS             complement(2023..2346)
                     /locus_tag="ACHOTW_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9846319.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2330..2683)
                     /locus_tag="ACHOTW_004334"
     CDS             complement(2330..2683)
                     /locus_tag="ACHOTW_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9846320.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2917..4107
                     /gene="nepI"
                     /locus_tag="ACHOTW_004335"
     CDS             2917..4107
                     /gene="nepI"
                     /locus_tag="ACHOTW_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP9846321.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4148..4441)
                     /gene="yicS"
                     /locus_tag="ACHOTW_004336"
     CDS             complement(4148..4441)
                     /gene="yicS"
                     /locus_tag="ACHOTW_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP9846322.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4663..5481
                     /gene="nlpA"
                     /locus_tag="ACHOTW_004337"
     CDS             4663..5481
                     /gene="nlpA"
                     /locus_tag="ACHOTW_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP9846323.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5485..6408)
                     /gene="yicL"
                     /locus_tag="ACHOTW_004338"
     CDS             complement(5485..6408)
                     /gene="yicL"
                     /locus_tag="ACHOTW_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP9846324.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(6519..6692)
                     /locus_tag="ACHOTW_004339"
                     /pseudo
     CDS             complement(6519..6692)
                     /locus_tag="ACHOTW_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6872..6977
                     /locus_tag="ACHOTW_004340"
                     /pseudo
     CDS             6872..6977
                     /locus_tag="ACHOTW_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            6988..7053
                     /locus_tag="ACHOTW_004341"
                     /pseudo
     CDS             6988..7053
                     /locus_tag="ACHOTW_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
BASE COUNT         1772 a         1609 c         1809 g         2055 t
ORIGIN      
        1 tcactgaacc gttgttgtca tttgttttac aaaagcaagg gattaaatct cctccaatgg
       61 acaaaaaaat gaataatgac aataccgatt acgtgagtaa tgaatcaggg acgctttcgc
      121 gattatttaa actacctcag catgggacca ccgtccgcac agaattgatt gcggggatga
      181 ccactttttt aaccatggtg tacatcgttt ttgtgaaccc gcaaatcctc ggcgcggcac
      241 aaatggaccc gaaagtggtg tttgttacca cctgtttgat tgccggtatc ggcagtattg
      301 cgatggggat atttgctaac ttacccgtgg cgctggctcc ggcaatgggg ctgaacgcct
      361 tctttgcgtt cgtggtcgtg ggggcgatgg gtatctcctg gcagaccggg atgggcgcga
      421 tattctgggg cgcaattggg ctatttttgc tcacgctatt tcgtatccgg tactggatga
      481 tctccaacat tccattaagt ttacgtattg gtatcaccag cggaatcgga ttatttatcg
      541 ccttaatggg attaaaaaat actggcgtta ttgtcgccaa taaagacact ctggtgatga
      601 ttggcgattt aagttctcac ggcgtgttgt taggtatttt agggtttttt attataaccg
      661 tgttgtcatc acgtcatttt catgccgcgg tgcttgtttc tattgtggtg acgtcttgct
      721 gtggattatt tttcggtgat gttcatttta gcggcgtcta ttccattccg cctgatatta
      781 gcggcgtcat tggtgaagta gatttgagcg gcgcgttaac acttgaactc gccggtatca
      841 ttttctcctt tatgctgatc aacctatttg attcatcagg aacattaatt ggtgtaactg
      901 ataaagctgg cttaatagat agtaacggta aattccccaa tatgaataag gcgctatatg
      961 ttgatagcgt agttcggtgg cgggcgcgtt tatcggcacc tcgtctgtta ccgcctatat
     1021 tgaaagtact tctggtgtgg cagtcggtgg tcgcacgggg ctgactgcgg ttgtggtcgg
     1081 cgttatgttc ctgttggtta tgttcttctc accgctggtg gcgatggttc ctccttacgc
     1141 aaccgccgga gcgttaatct ttgttggcgt gttgatgacc tcaagtctgg cgcgcgttaa
     1201 ctgggatgat tttaccgaat cggtgcctgc gtttgttacc acggtgatga tgccctttac
     1261 tttctcgatc accgaaggga ttgcacttgg ctttatgtcg tactgcatca tgaaagtgtg
     1321 caccgggcgc tggcgcgatc tgaacctgtg tgtggtggtg gtcgcagctc tgtttgcact
     1381 gaagattatt ctggtggatt agtaactggc gagggtttcc agatatcatg agttctgatt
     1441 acgcaggaga actcatgatc tggataatgc tcgccacgct ggcggtagtg tttgtggttg
     1501 gttttcgggt gctgacatcc ggggccagaa aagcgattcg ccgtctcagc gatcggctga
     1561 atatcgatgt cgtacccgtg gagtcgatgg tcgatcaaat gggaaagtca gccggtgacg
     1621 aatttttacg ttatttgcat cgtccggatg agtcgcacct gcaaaacgcc gcgcaggtgt
     1681 tgctcatctg gcaaattgtc attgtcgatg gtagcgaaca gaacctgctg caatggcatc
     1741 ggattttaca aaaagcccgc cttgccgctc ccattactga tgctcaggtc aggctggcgc
     1801 tgggttttct gcgcgaaacc gaacctgaaa tgcaggatat taatgctttt cagatgcgct
     1861 ataacgcgtt ctttcagcct gccgagggcg ttcactggct gcattaaaac cgcttcgtct
     1921 tatcagccta cagaccgcgc ctgacctgta gggtggataa ggcgttcacg ccgcatccgg
     1981 tatcggaact gacagtaggt gaacgcatta tctacgttct atttacagca gcacctccaa
     2041 cccgtgtttt tgcacaatgc tgagcagttt taatgaaatg ccattggggc gtttagcgcc
     2101 actttcccat ttttgtacag ttgaaacgct ggtattgaga tagtgagcga agacgggctg
     2161 actcactttt aatttttccc gcagtgcttt tatctcaaca ggttgtaagt cttgcacgct
     2221 ggtcaggcag gctttgtcaa aagtacgcat cgtttcagca gaaatagcat tgacgctgga
     2281 caatccagcc gctgcgctgt gaatagcctc aaatgcagga cttttaaatt tagtttttgc
     2341 agtcatagca gatctctgtc agttctttag tgttaatcat tgctgttagt tgggctttcg
     2401 tcaggaaggc ataatgcttt gcaatttcac gaaacccagc cagttcctga ctattgatgt
     2461 tcgacatatc ctgttttgca tataaaaacg tgtaaaacca attgcttacc cctttcgcca
     2521 gaataattgc acgctcacga ttttggtgaa ggcgcttttt aaaaacgcca ccgccaagtg
     2581 catcagcttt accttgttct gtttctaaaa tggcccggca caactcttca tctgttattg
     2641 catgagacct ggcttccctt gcaaaccatt tggttttaaa cacgcgtata tcgttgaggt
     2701 cctgttatta aagtatatca ccaagtgcaa tactttatcc cttgttccca acacaatcgc
     2761 gaaaatacat atcaccttgt cctatgattt ttatttcttc gagcagcctc gagcttttcc
     2821 cacaacaccg taaaagttgc aaaaacgtta aatgcgtcac acatttcaat gttaaagtgg
     2881 tcggcttttc ccctgaaaca tgccacgggt aacaccatga gtgaatttat tgccgaaaac
     2941 cgcggcgcgg atgccatcac ccgaccgaac tggtcagccg ttttctcggt ggcgttttgc
     3001 gtcgcctgtc tgattatcgt tgagtttttg cctgtcagtt tgttgacgcc aatggcccag
     3061 gatttaggca tttcggaagg ggttgccggg caatcggtga ccgtgaccgc ctttgtggca
     3121 atgtttgcca gtttgtttat tacccagaca attcaggcca ctgaccgccg ctacgttgtt
     3181 attttgtttg ccgttttgct gacgctctcc tgcttgctgg tttcctttgc taactcattc
     3241 agtttgcttt taatcggtcg tgcctgtctg gggctggcgc tgggcgggtt ctgggcgatg
     3301 tcggcgtcgc tgaccatgcg tctggtgccg ccgcgtacgg tgccgaaggc gctgtcggtg
     3361 atcttcggcg cggtttctat tgcgctggtg attgccgcgc cgttgggctg ttttttaggc
     3421 gagcttatcg gttggcgcaa tgtctttaat gcggcggcgg cgatgggtgt gctgtgtatt
     3481 ttctggatta tcaaatcact gccttcgctg ccaggtgaac cttcgcatca gaaacaaaat
     3541 actttccgct tattacaacg tccgggagtg atggccggga tgatcgccat ctttatgtcc
     3601 tttgccggac agtttgcttt tttcacttat attcgcccag tgtatatgac tctggcggga
     3661 ttcggtgttg atggcttaac cctggtgctg ctgagttttg gtatcgccag ctttgtcggg
     3721 acatcgcttt cgtcgtttat tctcaaacgt tcggtgaaac tggccttagc gggcgcaccg
     3781 tttgtgctcg ccctgagcgc gctggtactg acgctgtggg gaagcgataa aattgttgct
     3841 actggtgtgg cgattatctg ggggctgacc tttgctttaa ttcccgttgg ttggtcaacg
     3901 tggatcactc gctcattggc cgatcaggca gaaaaggctg gatctattca ggttgccgtt
     3961 attcagcttg ccaatacctg cggtgcggca atcggcggtt atgcgttgga taacattggt
     4021 ctgacttcgc cgctgatgtt gtccggcaca ttgatgttgc tgactgcatt gttggtgact
     4081 gcaaaggtga aaatgaagaa atcctgatat caggtaaaga agcgttggtc agtggttgtt
     4141 gccgttctta catatccggg cattctacct tacccaacgc ttcccaataa ttgctctgat
     4201 tattacgttc catcgccacg atggcctcgc gtatatgttg tttattgttt tcatctgaca
     4261 acattaactt ttcccacgct tcatctgata atgacgcctg tggcgtgcaa attttacgta
     4321 aatcttgcaa taccgtggtt ttctcttttg ccgactgtag agaactgaat ggcgactcgg
     4381 cctgagaaag ggctggaaaa atcaggaata cggtaatcat gagtagcatc gttggcttca
     4441 tagcggcaac cttctgcaaa tgtgcatcga atgaagcata agtgtagttt gcttttgccg
     4501 ctctcatcat gctgtacgaa aggtgtttgc gtggtcagta agaagtgcca gactttatat
     4561 tccactttta ttccttttta ttcttatcga tagcgttttg ttttttaaac tgcagcgacc
     4621 ttaccgctat agtccggtaa tcattaataa aaggataaaa aaatgaaact gacaacacat
     4681 catctacggg cgggggccgc attattactg gcaggagttc tgctggcagg ttgcgaccag
     4741 agcagcagcg atgaaaagca tattaaagtt ggcgttatta atggcgcaga acaagatgtc
     4801 gcagaagtcg ctaaaaaggt agcaaaagag aaatatggcc tcgatgttga actggtagga
     4861 tttagcggtt cgttgctacc gaacgatgcg acaaataatg gcgagcttga tgcgaacgtc
     4921 ttccagcatc gaccctttct tgaacaagat aatcagacgc atggttataa actggtagcg
     4981 gtgggaaata cgtttgtctt ccctatggcg ggctattcca aaaaaatcaa aaccgtggcg
     5041 caaataaaag aaggtgcgac agtggcgatc cccaacgacc cgactaacct tggtcgcgcg
     5101 cttttactgc tgcaaaaaga gaagctaatt actctgaaag agggaaaagg gttattacct
     5161 accgcgttgg atataaccga taatccacgc catttgcaga taatggagct ggaaggggca
     5221 cagttgccac gtgtactgga cgacccgaaa gtggatgtag cgattatcag caccacttac
     5281 atcgagcaaa caggactttc tccggttaac gacagcgtat ttattgaaga taaaaattcg
     5341 ccgtatgtga atattctggt ggcacgggaa gataataaga atgcagaaaa cgtgaaggaa
     5401 tttctgcaat cttatcaatc acccgaagtc gctaaagcgg cagaaaccat ctttaacggt
     5461 ggtgcggtgc ctggctggta attctcactc atgccgcgcc ggacgattta tttttctggc
     5521 gcggcggcgg gaatccattg aaatcaaaat caccgatgac agaatcagca gcgttcccag
     5581 ccagtccggc aatgtgaagg tgatccccag caacagcaaa gagagcagcg cgctacttaa
     5641 tggttcagca cagcttaaaa tgctggcttt tggaccgcca attaattgtg ctcctttcag
     5701 gtacagacta aatgtcaggg acgtaccaat gaccaccaaa taaaaaaacg ccagaatcaa
     5761 acttccgtta acgataaagt ttgtcccttg tccggcataa aagggcagca ggatcagacc
     5821 gccaatgagc atactccagc cgacgaccgg taacgtgcca tagcgggcaa tcagcgttga
     5881 gggataggtg gtatagaatg cagcagtaaa tgccgaggca atgccccaga ataacgcggc
     5941 aggagagatc gataatgacg tcggattacc gtgtgtcacc aataaaaaag tcccgatcag
     6001 cgatgtcaaa atagcgcaga aaaccagaat gcccgggcgg gatttacgca ccagtgagaa
     6061 ccaggcgacg ataatagtcg gtgagaggaa ttgcagcacc gttgccgtgg ctgcgttcga
     6121 tttttcgatg gttagcaaaa aagtgagctg tacggttagc gcgccaacca cggaaaaaat
     6181 cagtaggcta atggcatctt tgtgattgtt aataatagaa aagattttat caccatgaac
     6241 aaatgatagc gtcagcagaa tcaaaccggc gaatatcaaa cgcgtcatag tcaaaaactg
     6301 cgacgacatc tggctttgct ccatgatgta ttgcgcgcag acccctgaac ttccccacaa
     6361 cacggcggca atcagaacgt tcagcatccc ctttctggtg gaacccattt ttccctctgc
     6421 atactgtttt gtcgtttatg tatgcaggca tgatagcaaa aatggcgagg atggctcagt
     6481 ttgcgccacg ccgatttttc ttagcccaga ctggtgagct aaatatcttt aataaacagc
     6541 aagcaaatca ttgcaatacc caacatccct atcgccagcc agaacaacgt gtggtagctc
     6601 cagatttcaa ccataatttc gtcaacagag ccggcgataa tctagcctaa gcgtgaggta
     6661 tttgcatata acgttgttgc ggaacctatt cttatttatc ggagtgcctt gccagtaatt
     6721 tactgacgag gatcctcata atagcgttac ccatctgttg cattgagact ttacaggccg
     6781 ttattctaaa tgaccaaaat taaatcagtg cataagggga agtattctgt agtgatgtct
     6841 ccctgagatg caggttccac tggagaaaat tatgagcagt ttgtttcaga atgaaataaa
     6901 ggataaattt aaaactggac ttgcatatgt gcgggggcca gaaaaaagcg gatcttccgc
     6961 tcaaaatgca ggtagcgaag gtggtaaaaa atgcgccggg agaagcagaa gattgtcccg
     7021 cgatgtccgt ttgagaaaca cgagtttgat taaacgcgtg agcatgatga tattagtagc
     7081 tgaccagaga aattatatcc gtcgtaagca ataaaaatca gaagtgatgt tatggatatt
     7141 tttaacctga ataactccgt aagtacgggg ttattcaggt ttttgctagc ctgtgctttt
     7201 ctataccaga tgtggtgtga acactctatt ctgcatacaa attaa
//
LOCUS       DAZKIF010000189         7149 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZKIF010000189 DAZKIF010000000
VERSION     DAZKIF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.190"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            86..619
                     /locus_tag="ACHOTW_004342"
     CDS             86..619
                     /locus_tag="ACHOTW_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9846325.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            669..1352
                     /locus_tag="ACHOTW_004343"
     CDS             669..1352
                     /locus_tag="ACHOTW_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9846326.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVTFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSHNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            1365..3815
                     /locus_tag="ACHOTW_004344"
     CDS             1365..3815
                     /locus_tag="ACHOTW_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9846327.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLEHGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYIIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     ISNFDFIAGRSKIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDITHEGTSYQVAYNKYLVQTATRFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            3828..4787
                     /locus_tag="ACHOTW_004345"
     CDS             3828..4787
                     /locus_tag="ACHOTW_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFP9846328.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIAL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNFNLNTILQVNLMTCGFEDQSIDTGLFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(4825..5724)
                     /gene="nhaR"
                     /locus_tag="ACHOTW_004346"
     CDS             complement(4825..5724)
                     /gene="nhaR"
                     /locus_tag="ACHOTW_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP9846329.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(5790..6956)
                     /gene="nhaA"
                     /locus_tag="ACHOTW_004347"
     CDS             complement(5790..6956)
                     /gene="nhaA"
                     /locus_tag="ACHOTW_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP9846330.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         2231 a         1573 c         1462 g         1883 t
ORIGIN      
        1 acattaatct aaataataac aacctgatca cataatcggg tttgaaaacg agtgaccttc
       61 cataccagtt aattaaggat gatttatgaa aaagtatatt attcccttcg ttgcgacaac
      121 gttgatgttt agcgcaacaa ccttcgccaa ggatgtcgat caaggaacac taaccattag
      181 aggagaagtt gttgatacaa cgtgttactt tgttaatggt gattcgacaa cagatatcgt
      241 tgtcgaagat gttgtaacca gtgcaatgaa tcaacttatc aataatgaaa tctatacttt
      301 aaaaaccggt tcaacctctc atgacttaga aattcagtgt gacggaaata cagccccaag
      361 tatcgaagta ctagcatcag agtttgatgc taataacttt acgctgaata aaggaactgc
      421 tgagaacatt ggctttgcca ttttcctcga tgatgggact ttgaccagaa tgagaccagg
      481 gttaaaaaca ccgctgaaag ataatggctc tggtcaatat tttctcaatt taaatgcaca
      541 atatgcccgc acaagtggca atcctgttac aaaaggctcc gtagagtcaa ccgtaacgat
      601 taaaatcagt gcagattaac gttttttgcc ggtagctctg ctaccggtat tatttacgga
      661 gtgattacgt gagaataata acagcattac tgatgtcatt ttttatttta cctgttcatg
      721 ccggtgtggt catctatggt acgcgcatta tttatccgga aaaaaacaga gaagttctcg
      781 tacagttaat gaatcaaagt aaaaatgcct ctttgattca ggcatggatc gatgatggga
      841 acaccactat cgcgccagaa aaaattcagg tcacatttat tctcacaccg ccagtaagtc
      901 gtgtagcagg tggttctggc caacaactta aaataagaaa aatgccaaac aatttgcctc
      961 acaataaaga aagtttattt tatctaaatg ttctggatat ccctccaaac aatccacaaa
     1021 atgcagggaa aaacaaaatt aaacttgcat tgcaaaaccg gataaagtta ctttggcgtc
     1081 caagcggaat agctccggta gataaaaaaa gcctttctca attaaatatc aaaaagaaaa
     1141 ataacgccat ctcaataaac aacgaaacag ccaactggat aacggttaca actatcaaag
     1201 cacaaaacgt gaaagttaat aatgaaagta tcttactgcc tcccttttcg cataacgaca
     1261 ttacattaaa aaataaccac gcatcagaat atgaactaac ggtagttgat gattacggga
     1321 ataacatcca tagtaaaatc gccgcgagat aacaggaaaa agtcatgtta aggacgacac
     1381 gatgggtcgc tgctattatt tttttgtatt cttttccggg ttatgctgaa gaaacttttg
     1441 atacgcactt tatgatcggc ggcatgaaag gagaaaaagt ttccgaatat catttcgata
     1501 ataaacaacc gcttccagga aactacgaac ttgattttta tgtaaataac cagtggcgtg
     1561 gaaaacaaga tatcacgatc ccagagtcac cagtcaaacc atgtctgcct aaggtgctac
     1621 taaccaaact gggggtaaag accggtaacc tcaatacaga agataattgt attttactgg
     1681 ataaagcggt tcatggtgga caatatcagt gggatatcag tgaacaccga ctaaatttaa
     1741 cagtaccaca ggcgtatatc aatgaacttg aaagaggtta cgttcccccg gaaagttggg
     1801 atcgcggcat tgatgctttt tatacctcct ataatttaag tcagtatcgc tcttatgaca
     1861 gcaataataa cagtaataca gccagttatg gccgatttaa cagtggatta aatttattta
     1921 gttggcagtt acactctgat gccagctata gcaaacccga tgatatgaaa ggcacatggc
     1981 aaagtaatac tctttacctg gaacatggct ggtcgcagat attaagcaca gttcagatag
     2041 gagaaaatta cacatcctca ctcatattcg actctctgag atttagcgga atacgcttat
     2101 ttcgcgatat gcaaatgcta cctgattcta tgcagagctt tacaccttta gtacaaggtg
     2161 ttgcgcaaag taatgccttg ataacggttt cgcaaaatgg ctacatcatt taccaaaaag
     2221 aggttccccc gggaccattt actatcgcag atttgcaact ctcgggcagc ggctctgatc
     2281 tcgatgtcag cataaaggaa gcagatggca gtgttcgttc ctttttggtg ccctactctt
     2341 ccgtgcctaa catgctgcaa cctggtattt caaatttcga ttttatcgcc ggacgcagta
     2401 agatatatgg tgtaaagaat caggaggact ttctcgaggc aaattatata tatggcctca
     2461 acaatctttt gacactttat ggcggtacga ttttatccga taactataat gctataactc
     2521 tgggaaatgg ttggaataca ccgctgggtg ccatctcttt tgacgctaca cgatcgagta
     2581 gtaagttaaa taatgatata acacacgaag gtacgagcta tcaggtcgcc tataataaat
     2641 atctagtaca aacggccact cgatttagcg tggccgcctg gcgttatgct tcgcaggatt
     2701 acaggacatt cagcgaccat ctttacgaaa atgataaaat aaatcatcag agtgactatg
     2761 acgattttta tgatattggc agaaaaaata gcctttctgc caatatcatg caacctttat
     2821 ccaataatct gggaaatgta tcattaagtg cgctttggcg gaattactgg gggcgaagtg
     2881 gaaatgctaa agattaccaa ttcagttatt ccaatagctg gcaacgcatc agttatactt
     2941 tctctgccag ccaatcctat gatgaaaacg acaaagaaga ggagcgtttt aatctgttta
     3001 tctccattcc tttctactgg ggggatgata ttgccaaaac acgtcaccaa attaacttat
     3061 cgaattcgac ctcattttcc aaagatggct attcctccaa caatactgga attactggca
     3121 tagccggtga acatgatcag ttaaattatg gtatatatgt taatcagcaa caacaaaata
     3181 atgatacatc gcttggtacg aatttaagct ggagaactcc catcgccaca atagatggca
     3241 gctatagcca ttctaaaaac gcctggcaaa gtggtggaag tattagtagt ggattagttg
     3301 tctggcccgg tggtattaat atcactaacc agctatccga tacatttgca attctggatg
     3361 cgcctgggtt agaaggcgcg catattaatg gacaaaaata caaccgaaca aacagcaaag
     3421 gccaggttgt ttacgacctg atgatacctc atcgtgaaaa ccatctggta cttgatacag
     3481 caaacagtga aagtgaaaca gaattgcagg gcaatcgtca aattattgcg ccttaccgtg
     3541 gagcagtttc ttatgtgcag tttacaactg accaacgtaa gccctggtat atacaggcac
     3601 tgcgtcccga tggttcgcca ttaacttttg gctatgacgt actggatctc caggaaaaca
     3661 atattggagt cgttggccag ggtagtcgcc tttttattcg cgtagatgaa attccaactg
     3721 gcataaaagt tgctctcaat gatgaacaga atttattctg tactattact tttcaacacg
     3781 ttatcgatga aaacaaaaca tatatatgcc agtaaaagga gtttactatg aagtggcttt
     3841 tattaatcac attgagtcta tattctttta ttgtgcaaag tgctccctgt ggattgacca
     3901 atgtcgggga acaacgtgga acatatatac tcaaaccact gagtatgaaa ggtaatctca
     3961 ccgccgaaaa aattgaaaat actgggaata tcattgagtg tgcagacttg caagagacga
     4021 ccaacgccat taaagttcat tttttaacac agaacgcttt aaggtttaaa gtcggaagtc
     4081 gtcattatat tattaatttc ccctttgaat ctgacggtaa aaaaatagcg cttccaggga
     4141 agtcatctta taatttagaa gaaatgctat ctgcgataaa tttcaccatt ccatactcta
     4201 tcgcatctgt atcagatagt tccaacaccg cagatatttt tccaggaact ccttttcctt
     4261 tgttgttcga aattgaagtg agttcatgta cagggaataa tactaattgt aatagcgtcc
     4321 gctttaattt taatttaaat actattttac aggtaaattt aatgacctgt ggttttgaag
     4381 atcaatccat cgacactgga ctatttgtac tgtctcgtat caataatgaa aattatcagt
     4441 ttcatcatat aagatttgac tgcatccagg gcgagcaagg tgaattagtt tttgaaccca
     4501 gtgacgttga atattacttt gaaccagtga cgatacagga gtcaggaacc agcatattaa
     4561 aaaatgagtt agaaaacagt gaggacggtg cgggtcaaat aggctttcaa ctctccaatg
     4621 atggtacgaa tgaaatccag tatggtaaaa gcaattacta ctcttttcag catccccatg
     4681 aaggaagtaa tcagattcct ctatttattc gcccgcgtac atatggaaac aatgtctcaa
     4741 gcgggcaaat aatgagccga gtcaaaatcg tggtaatgta caactaatct atctccccca
     4801 ccttaacttt gggggagctg ccgattaacg caccgctgga ctaaaaagcg cagaataatc
     4861 cgtattgcag attcgctgta ccgccgggtg ctgaatcatc cgctcagcaa aaatagcatg
     4921 gtactcttcc atcacattct cgacgcgacc aatttctacg acagttttat cggcataaaa
     4981 gtcatatgca taaagcgttg gggcaacgaa gattgcattg tgcatcgcac caaaagcttt
     5041 catcaaagcg gcatcatcaa actcgccaag gatttctacg tttaatccct gggagttaaa
     5101 ccagttaagt aatttgcgcc ctaacattga acgtcgccca ggaatcaaaa gccgccgttc
     5161 ttcaagacaa gctgggaacg gtttttccgg cggtggattt gtacaccaga aactcacgcc
     5221 acattcgcca attctcacgg agaacaggcc ttcctgctgc gtagaatcta tcggacagtc
     5281 ggaaatgatc atatccagtt tatgctggct taattgctcc agcagcattt cgtgggtgga
     5341 ttcgaagcag cgaagatgaa tcggctcgcc ttctactact gcagcattca gcacgctact
     5401 gaccagacgt ttggaaagta catcagccac gccaacgtca aacaataaat tggattcttt
     5461 gcgatagttc acgatatcca gcatttcctg gctcaaggta aacattttat cggcatagcg
     5521 atagaccagc tcccccagct cgctgggctc gagaccacgt cccttgcgct taaataattt
     5581 gccttgcagg cgctcttcca gcgcccgaat ctgtccggta atggtttgtg gtgttaaata
     5641 aagcgcctcc gctgcgccaa ccacagaacc ttctttataa acatgccaga agtaatacaa
     5701 gtggttgtaa ttgatatgag acatgctcat ttctctccct gataacaatg aaaagggagc
     5761 cgtttatggc tccccggtaa accgtcctgt caaactgatg gacgcaaacg aacgcgtaac
     5821 cagctgtatc caattaccgc cgaagagata gaaccgacca ggataccgag tttcgcccag
     5881 ttaatcagtt ctggatctac gctaccaaag gccaggctgg caataaagat agacatagta
     5941 aaaccgatac cgcacaggat ccccaccgcc ataatttgct gataagtcgt tccctcaggc
     6001 agatgcgcca gtttcaaacg cagcgccaac cagcagaaca gactaatccc cagcggtttg
     6061 ccaatcagca agccagcgat gatccccaat ggcagaatgg aggtcaagcc atccagcgtg
     6121 acgccttgca gtgaaacgcc agcattagca aatgcaaaca gtggtaaaat cagatacgcc
     6181 acccacgggt gcaacacatg ctccagtcgc ttcgctggag aacgcccatg cttctctttc
     6241 aaaggaataa agaagccgac aattaccccc gccagggttg cgtgaacccc cgatttcaac
     6301 actgcagtcc acaacactac gccaaccaga atatagacgc ccgtgcggcg tacaccacac
     6361 agattcaata ccgcgagtac agcaattgct acagccgcga cgccaagaga ggccatcgat
     6421 aagtcattag tgtagaacaa tgcgatgata atgatggccc caagatcgtc gataatagcc
     6481 agagccatca aaaagatctt cagcgctaac ggaacacgac ttcccaatag cgccagcaca
     6541 ccaagtgcaa aggcaatgtc agtagccgcc gggatcgccc acccttcgcg ggtaatcgga
     6601 tcggcatagt taaaggccag atagagtaat gccggcacaa tcatcccacc aatagcagcg
     6661 ataactggaa atgcggcctg gcgtaagctg gctagcgatc cttgcatcag ttcacgttta
     6721 acttccagac cgactaacag gaaaaatacc gccatcagcg cgtcatttat ccataacagc
     6781 atgtttttgt tgatttcgag tgaaccaacc cggagctgaa ccggcgtctc cagaaagtcg
     6841 tgataccatc cactggttgc gccgctgttg gccatcatca tcgccaggat agcggcaatg
     6901 ataagaataa tgcctcccga ggcatcactg ctaaagaatc gatgcagatg tttcactttt
     6961 tatttctctt tcaggtgaat agatcgtttt tactatttta ccccgcacga atcatcgtta
     7021 aaatcagatt atagtgtagg aataattcgt tttttacgat ctatagaaga atttgcgcca
     7081 tcagcggtga gatgcgtttt tgacggctgt aagcgctgac gggtgaccct aagccctgat
     7141 aatcatagc
//
LOCUS       DAZKIF010000190         7138 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZKIF010000190 DAZKIF010000000
VERSION     DAZKIF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.191"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(329..1465)
                     /locus_tag="ACHOTW_004348"
     CDS             complement(329..1465)
                     /locus_tag="ACHOTW_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9846331.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1468..1830)
                     /locus_tag="ACHOTW_004349"
     CDS             complement(1468..1830)
                     /locus_tag="ACHOTW_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9846332.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2367..4280
                     /gene="mtlA"
                     /locus_tag="ACHOTW_004350"
     CDS             2367..4280
                     /gene="mtlA"
                     /locus_tag="ACHOTW_004350"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP9846333.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4375..5523
                     /gene="mtlD"
                     /locus_tag="ACHOTW_004351"
     CDS             4375..5523
                     /gene="mtlD"
                     /locus_tag="ACHOTW_004351"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9846334.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5523..6110
                     /gene="mtlR"
                     /locus_tag="ACHOTW_004352"
     CDS             5523..6110
                     /gene="mtlR"
                     /locus_tag="ACHOTW_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP9846335.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6122..6331)
                     /gene="yibT"
                     /locus_tag="ACHOTW_004353"
     CDS             complement(6122..6331)
                     /gene="yibT"
                     /locus_tag="ACHOTW_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP9846336.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6616..6978
                     /locus_tag="ACHOTW_004354"
     CDS             6616..6978
                     /locus_tag="ACHOTW_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP9846337.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1820 a         1787 c         1806 g         1725 t
ORIGIN      
        1 ttgtgatcta tagattaaat attttttaaa tgtgactgtc atccttcaga taataaagcc
       61 atcatcgcaa tagatggaaa agcactcctg ctcttttttg attagagccg tcgcaggagg
      121 aacaactcat atcatcattg cgatctcgac gatgtatagt ctcatcctgc ggcagaacaa
      181 gacggataaa aaatccaatg agagcacact catccagaac ttattaacat aatgaatatt
      241 aaagaaaaat catcgcagct aatgaagtaa tatgcaaaaa taattagtcc ttacaaggag
      301 caaagttcca taatctcccc cctccccctc aatgatccaa ataaagataa tgcatccagc
      361 tggtcattct tagcagcact ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa
      421 ccgccacctg ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta gggtccagtt
      481 caatggttcc cagcacaccg tccgtgccgg gcacgaccgt taatgattgc aataccccct
      541 gcgcctgata agaaccgcct ggcacgacag gtaaaatact agtcagtttg ccgtgaaaca
      601 cctgcccagg tagcgcgtta aacaccactt ccgcatcatc gccgggtttc agacgtaaca
      661 gcgagttttg ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aagaccatca
      721 ccggacgcag cggcaaggca gctgcgtatg tacctgggcg gatcagtacc tgagtaacgt
      781 agccattgct cggcgcgcga atgacagtct gctcaaggtt atattttgct tcagtaagtt
      841 gcgctcttaa gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct
      901 ggatctgcgc ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt
      961 gccgcgcatc gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca
     1021 agtaacgttg ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg
     1081 cctgcgcttc agtgagctgc gcacgcagcg tctttatatt atgcgtcgcc gtcatcaggt
     1141 cagcctgaag tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct
     1201 cgcccttttg aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct
     1261 gtggcgtgat agggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt
     1321 agttcatcaa taaaatcaaa ccactcacca gaaacacgcc tcccagcgcc gccgtcgcca
     1381 gcgtccactg atttaccgga atgcggaaga ttttaaagac cgcccacgcc agcgccacgt
     1441 aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat agtcagtctt
     1501 tttctcggcg gcgagttgat gctccagccg ggaaattcgg tcagaaagtg aggcgatttc
     1561 cgggtcagtc gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg
     1621 ttgatagagc gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct
     1681 cacccagccc gccgtatgaa tagcgtcggc atgggggtga ttgcgctttt tggcaatcag
     1741 ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag
     1801 cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt
     1861 gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg
     1921 cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct tagttacata
     1981 acgattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa
     2041 atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgacacta
     2101 ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt tacacaagcg
     2161 ttttgtgatg aacgtcacgt caattacctc tctaccccct atatttatgt gattgatatc
     2221 acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac
     2281 aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga
     2341 cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct
     2401 ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta
     2461 tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg
     2521 tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg
     2581 taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt atcgtcggcg
     2641 cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta
     2701 agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata
     2761 acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc ggcattggcc
     2821 cgattgttga aaccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg
     2881 acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg
     2941 ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat
     3001 caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca
     3061 tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc
     3121 tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctcg
     3181 cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg
     3241 tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt
     3301 atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta
     3361 ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta
     3421 tgcaggacat gaaagctgag tctaaaggtg catctccgct gtctgcgggc gatgtgacta
     3481 acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttccagtg
     3541 cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca cagatttcgg
     3601 tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg
     3661 acctgaccga acgcgctatg cgccaggttc ctcaggcaca gcatatttcg ctaaccaact
     3721 tcctcgacag cggcctgtac accagcctga ctgaacgtct ggttgctgcc cagcgacata
     3781 ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat tccagtgcta
     3841 acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca accaaagaag
     3901 aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggagtacg
     3961 ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg
     4021 tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg gtgttctgcc
     4081 agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgctcgt ctggtgattg
     4141 gtattgctgc ccgtaacaac gagcacattc aggttatcac cagcctgacc aacgcactgg
     4201 atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac
     4261 tgctggcagg tcgtaagtaa tccaatccta ccctctcctc acggagaggg tttgggtgag
     4321 ggaaaagcct caccccagcc ctctcgggta aaaacattga tgaaggttaa tactatgaaa
     4381 gcattacatt ttggcgcagg taatatcggt cgtggcttta tcggtaaact gctggcagac
     4441 gcgggtatcc aactgacgtt tgccgatgtc aatcaggtgg tacttgatgc cctgaatgcc
     4501 cgtcatagct atcaggttca tgtggtcggt gaaaccgagc aggtagatac cgtttccggc
     4561 gtcgatgctg tcagcagcat tggtgatgat gtcgttgatc tgattgctca ggtcgattta
     4621 gtcactaccg ccgttggccc ggttgtgctg gaacgtattg ctccggctat cgccaaaggg
     4681 ctggtgaaac gtaaagaaca aggtaatgaa tccccgctga acatcatcgc ctgtgaaaac
     4741 atggtacgcg gtaccacgca gctgaaaggt catgtgatga acgccctacc agaagacgcc
     4801 aaagcgtggg tagaagaaca cgttggcttt gtcgattccg ccgttgaccg catcgtaccg
     4861 ccttcggctt cggcaactaa cgatccgctg gaagtgacgg tagaaacttt cagcgaatgg
     4921 attgtcgata aaacgcagtt caaaggcgca ctgccgaaca tcccaggcat ggagttaacc
     4981 gacaacctga tggcatttgt cgaacgtaaa ctcttcaccc tgaacacggg tcatgctata
     5041 accgcgtacc tcggaaaact ggccggtcat cagaccattc gtgacgctat tctcgacgag
     5101 aaaatccgcg cggtggtaaa aggtgcgatg gaagaaagtg gtgcggtact gatcaagcgc
     5161 tacggctttg acgcagacaa acatgcggcg tacatccaga aaatcctcgg tcgttttgag
     5221 aacccgtatc tgaaagatga tgtagagcgc gtaggccgtc agccgctgcg taaactgagt
     5281 gctggcgacc gtctgatcaa gccattgctc ggtacgctgg aatacagcct tccgcacaag
     5341 aatctgattc aggggattgc tggtgcaatg cacttccgca gtgaagacga tccgcaggct
     5401 caggaactgg cagcactgat cgctgacaaa ggtccgcagg cggcgctggc acagatttcc
     5461 gatcttgatg ccaacagcga ggttgtatcc gaggcggtaa ccgcttataa agcaatgcaa
     5521 taatggtgga ccaggcgcag gacaccctgc gcccgaataa cagattgtca gatatgcagg
     5581 caacaatgga acaaacccag gcctttgaaa accgtgtgct tgagcgtctg aatgctggca
     5641 aaaccgtgcg aagctttctg atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc
     5701 tggtgcttca ggtattccgc aaagacgatt acgcggtgaa gtatgctgta gaaccgttac
     5761 tcgacggcga cggtccgctg ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg
     5821 gcgtcattaa ccgccaggaa tacgaagatg cggagctgct gatggcattg cgtgaagaac
     5881 taaatcacga cggcaacgag tacgccttta ccgacgacga gatccttgga ccctttgggg
     5941 aactgcactg cgtggcggcg ttaccaccgc cgccacagtt tgaaccagca gactccagtt
     6001 tgtatgcaat gcaaattcag cgctatcaac aggctgtgcg atcaacaatg gtcctttcac
     6061 tgactgagct gatttccaga atcagcttaa aaaaagcctt tcaaaagtaa gcaacgtctg
     6121 cttactgccc ctctacctgc ttcggccgat aaagccgacg ataatactcc agacgttgta
     6181 gatataacgg cacgctttca tcgggtattc cggacggaat cgcgttacgg ggaggaagtt
     6241 ttttcagata ctcccggaac gcctggcttg atgccatgaa atctacggct ttatcgataa
     6301 gaagcggaac gttttcacct aatttgccca tgatgatatc tccgtattac cccgcgccgg
     6361 gaatgcgcgg ccgccaattt tagtttaggt tcccggtaaa gatgacatta aggaaaacgt
     6421 gctgaatcct caataatcat ccgctgctgt ttattatctt ttttctatag atttcttaat
     6481 taatcaataa attgcgtagt ttttaagcat gttaatgatt acgttacaga actattgcaa
     6541 tgtcatcagt tgtgaacaaa gcacctggtc gcgcatacta ggggctataa atttatcttt
     6601 atcagaagcc atcacatgaa agaagtcgaa aaaaacgaaa tcaaacgtct cagcgatcgt
     6661 ctggacgcca tccgccacca gcaggccgat ctgtcgctgg ttgaagccgc agacaaatat
     6721 gccgagctgg aaaaagagaa agccacgctg gaagcagaaa ttgctcgcct gcgtgaagtt
     6781 catagccaaa aactgagtaa agaagcacag aagctgatga agatgccgtt tcagcgcgca
     6841 attaccaaaa aagagcaggc tgatatgggc aagctgaaga aaagtgttcg cggactggtg
     6901 gttgtgcacc caatgaccgc gctgggccgt gaaatgggcc tgcaggagat gactggattc
     6961 tctaagactg cgttttaagc gcctcagacg actggttcga tcatatcaac ccgcatctgg
     7021 cttgccttag ctggtcaatg cggggactct cctgtctact cgcctaatta ccaaacaatt
     7081 cactcccttt acgtcgattt accgctggtt gatattctga aaatgcattt ttaccgaa
//
LOCUS       DAZKIF010000191         7078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZKIF010000191 DAZKIF010000000
VERSION     DAZKIF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.192"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            96..746
                     /gene="csgD"
                     /locus_tag="ACHOTW_004355"
     CDS             96..746
                     /gene="csgD"
                     /locus_tag="ACHOTW_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP9846338.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            751..1140
                     /gene="csgE"
                     /locus_tag="ACHOTW_004356"
     CDS             751..1140
                     /gene="csgE"
                     /locus_tag="ACHOTW_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP9846339.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1165..1581
                     /gene="csgF"
                     /locus_tag="ACHOTW_004357"
     CDS             1165..1581
                     /gene="csgF"
                     /locus_tag="ACHOTW_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP9846340.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1608..2441
                     /gene="csgG"
                     /locus_tag="ACHOTW_004358"
     CDS             1608..2441
                     /gene="csgG"
                     /locus_tag="ACHOTW_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP9846341.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2505..2996)
                     /gene="ycdZ"
                     /locus_tag="ACHOTW_004359"
     CDS             complement(2505..2996)
                     /gene="ycdZ"
                     /locus_tag="ACHOTW_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP9846342.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3098..3652)
                     /gene="ycdY"
                     /locus_tag="ACHOTW_004360"
     CDS             complement(3098..3652)
                     /gene="ycdY"
                     /locus_tag="ACHOTW_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP9846343.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3676..4413)
                     /gene="ycdX"
                     /locus_tag="ACHOTW_004361"
     CDS             complement(3676..4413)
                     /gene="ycdX"
                     /locus_tag="ACHOTW_004361"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP9846344.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4468..5406)
                     /gene="ghrA"
                     /locus_tag="ACHOTW_004362"
     CDS             complement(4468..5406)
                     /gene="ghrA"
                     /locus_tag="ACHOTW_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP9846345.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5640..5727
                     /locus_tag="ACHOTW_004363"
     tRNA            5640..5727
                     /locus_tag="ACHOTW_004363"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5674..5676,aa:Ser,seq:gga)
     gene            complement(5877..6719)
                     /locus_tag="ACHOTW_004364"
     CDS             complement(5877..6719)
                     /locus_tag="ACHOTW_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9846346.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6804..7001)
                     /locus_tag="ACHOTW_004365"
     CDS             complement(6804..7001)
                     /locus_tag="ACHOTW_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9846347.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7013..>7078)
                     /locus_tag="ACHOTW_004366"
     CDS             complement(7013..>7078)
                     /locus_tag="ACHOTW_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9846348.1"
                     /translation="VSLIHLDASAECLPGLPTFDW"
BASE COUNT         1862 a         1754 c         1643 g         1819 t
ORIGIN      
        1 acacacagca gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc
       61 aggtgtgcga tcaataaaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
      121 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
      181 accttaaaca atcgctggca attacaggaa aattacataa tattcaacgt tctctggacg
      241 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
      301 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
      361 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
      421 atgccatgga ggatcaagaa cgcgttgtca atggattgca aggcgtcctg cgcggcgaat
      481 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
      541 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
      601 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
      661 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
      721 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
      781 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
      841 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
      901 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
      961 agctggatca caataacggt caatcaggac gttattttcc agactttttt attcccgttg
     1021 aaaagagact tcgaaaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     1081 cgtcgccaga taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa
     1141 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     1201 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     1261 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     1321 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     1381 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     1441 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     1501 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa
     1561 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     1621 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     1681 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     1741 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     1801 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccacggcg atgctggtca
     1861 cggcattgaa agattctcgc tggtttatac cgctggagcg ccagggctta caaaacctgc
     1921 ttaatgagcg caagattatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc
     1981 gaatcccgct gcaatcttta actgcggcaa atatcatggt tgaaggttcg attatcggtt
     2041 atgaaagcaa tgtcaaatct ggcggggttg gggcaagata ttttgggatc ggtgccgaca
     2101 cgcaatacca gctcgatcag attgccgtga acctgcgcgt cgtcaatgtg agtaccggtg
     2161 agatcctctc ctcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg
     2221 ttttccgctt tattgactac cagcgcttgc tggaagggga agtgggttac acctcgaacg
     2281 aacccgttat gctgtgcctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg
     2341 atggtattga ccgtggtctg tgggatttgc agaacaaagc agaacggcag aatgacattc
     2401 tggtgaaata ccgccatatg tcggttccac cggaatcctg acagataaaa atgccggaat
     2461 taataatccg gcatttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg
     2521 atttcctgct cacggagagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt
     2581 ttcattgcgt aaccaaatat cagcccaagc gccagcgaag gtaacaccag tttccagtca
     2641 ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc
     2701 agctgtttgg cctgaataca catcagaaac gcgacaatac cggttatgac ataactgaga
     2761 atttccagat gtggcgccag tgcactaccg taaataatga ccatcgccca caccacgcca
     2821 ctaagcagag ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaggcaaag
     2881 taggccgtac agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat
     2941 ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg
     3001 cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacagca
     3061 aattatctgt tgcagttatg agtgacgcat atcacattta ctcttcagaa tcttcttcca
     3121 gctcgtccca cattgcacta atggcatcgc gggttagcgg tgccatggtg cgccagaaag
     3181 gcgtggttgc atgggcctcc actttgccaa ggaacgcacc acaccagggt aacagatact
     3241 cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg
     3301 aagccgcaag cagcaatgtg ccgatgtgat cggcaggcgt atccgctaat ggcatccctc
     3361 gttcagaaag aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat
     3421 atggcggcac ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg
     3481 tcatatcaca gcttttctgc aaacgtgtca gcaactcatc ctgctccagt ggccagttcg
     3541 cagccagttt cccctcacga atcagggtaa acagcggcac cagtaaagga tcttgcggtt
     3601 ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat
     3661 ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc
     3721 aaggaagttc agtaagcggc gcggagaaac attcaaaatg cgctctggcg gaaaatctac
     3781 cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc
     3841 cgagcctaat gccacccaac ctccagcatc acgtaccgct gcggctaccg cacgacagtt
     3901 gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta ttgatttcca gcgccacctg
     3961 atgtttcgcg gctgcttcag caacggcttt aacatctatc tcatatttcg gatttccggg
     4021 atggcttatt atatgcacat tgccgctggc gatagtggcg atcatcgctt gtgtattggt
     4081 cgctttgtca tgtggtgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga
     4141 gtcaaacatt ttaccgctgc aatcaatttc accatcaaca tttttaatgt tagcttcgat
     4201 gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg
     4261 ccagtgatgc ggcgcatctt ccatgtccgg gccatgatcg gtaatcgcaa aaagtttaat
     4321 gcccttttgt ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc
     4381 aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc
     4441 aaggatagca ggaatcctga tgctttatta gtagccgcgt gcgcggtcga cttgcccgca
     4501 gaccctctcc cctttttcga gctgggcaat agtgcgagaa atgtactcca cagcttcagc
     4561 gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg
     4621 actttcaggc ggtaagggtt cacgattaaa aacatccaac attgcgcctt taactttgcc
     4681 gctatccagc gccgcgagca ggtcatcttc cacgacatga acaccacgcg ccaggttgag
     4741 gagatacgcg ccatccggta atttttcgag taattgttga ttaataatgc cgacggtttc
     4801 aggggtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc
     4861 ttcccatccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg
     4921 cagcggaaag cgccaggttt gcagactctg agcaacttta ctgcccagta cgcctgcgcc
     4981 caaaatgccg atggtaaaat cttcccgatg atattcaggc agcggttgcc aatgcgaact
     5041 attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc
     5101 atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg
     5161 cttcagcatt tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag
     5221 tgcgaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat gccaggctaa
     5281 agcataatca gcagagtcat tatctccgct tttccatgct ctgactcttg cctgaggaat
     5341 agctttgcgc agtgcctcta tccaccattg ggtatcgaac gttgggtgat aaaagatgat
     5401 atccatactg actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct
     5461 acaaagacca ccagcataac aaatccgtgg tcggtggcca aaaaagcaga ttttgcttat
     5521 taaaactgca tattgattga agtttgaata aacgcgcgat tttttaaaaa agtttgttga
     5581 cctcaggtca tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg
     5641 gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg
     5701 ggggttcgaa tccccccctc accgccagat tataagcgca tgttattgtt taacatgcgc
     5761 ttttttattt tcccttccag acagtatgcc atcgggtatg ccatctgaaa ccggtttata
     5821 tgccccgcga tcatatctta tcggacaatt tccgatcttt gagcatactg gcaaagctat
     5881 actgccagca tatcaggata gtgttttgcg ataatatcat tcagtctgtc aaccagttct
     5941 ggttttctga acgtgatatg cgcggagccc ttcttaaagt atctgatgct gaacatctca
     6001 tcttcatagc tctctttacc ctgaacagac tgaatgtgat catccagacg aagggtgatg
     6061 tttccccggt tgtcggggat tggtttaccg ctgaacaggt gcaacattct ttccagatcg
     6121 gcgagtcggt cagcctgttg tccggtgata agatgaaatc cccaccggtc ccatctcacc
     6181 agattgttga cgataatttt actgccaaat ttacaggggc aattggtttt gtaattccag
     6241 ttcagccctc tgaagacgtt gatcacccct cgctcaaaca cctcatcctt attctggtgc
     6301 agttgttcaa atgtgctcag aatgttcgct tcgctgattt ccggaaaatt atcgtactcc
     6361 agtgaccggt accatgtatc acgggcctgc gtatccatta atgacagcat cccggaacgt
     6421 tgcatcaggt cacgccagat ttcgcgatca agattccggg taatggcttt cattgctgtt
     6481 tcgggtttct ccatcagcca gcagccgcag cgaaaatcct gtttcatggc ccagtccggg
     6541 gcagttttac ctccgatgct ttccgtcagc accgagattt cagccagttg tcggataagt
     6601 gtgtcgattt gcaacagtgt actctttcgt acagccagaa tgtggcgaat gctggttgag
     6661 cagataacgt ccgtatgccc gttgaccact tccggacggg agattgtgat atctgacata
     6721 atcgatattc ctttttgcag ataaatgaaa acgctagccc tgtgcaggct ggcgtcagag
     6781 gaatgaagtc tggtgagtga gcttcaccgg ttctggtgca gaagctgcag atgacgcagg
     6841 gtacggacat cctcccgggc ctgttcgata cagggcaaca gtgctgctga atccgtttta
     6901 tcctcatcgt tgtcaaagat aattcccgat ccgcagtcga tattgtcctg cagccacgca
     6961 atcagaatat ccagcgctgt ttccgtggtt aatgatttca tgttgttcct ggttaccagt
     7021 cgaaagtggg taaacccggc aggcattcag cgctggcatc cagatgaatg agactgac
//
LOCUS       DAZKIF010000192         7049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZKIF010000192 DAZKIF010000000
VERSION     DAZKIF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.193"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..485)
                     /locus_tag="ACHOTW_004367"
                     /pseudo
     CDS             complement(1..485)
                     /locus_tag="ACHOTW_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(719..1459)
                     /locus_tag="ACHOTW_004368"
     CDS             complement(719..1459)
                     /locus_tag="ACHOTW_004368"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP9846349.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            1388..1852
                     /locus_tag="ACHOTW_004369"
     CDS             1388..1852
                     /locus_tag="ACHOTW_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846350.1"
                     /translation="MPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGTFFS
                     LVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRTVTE
                     VNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            complement(1784..1987)
                     /locus_tag="ACHOTW_004370"
     CDS             complement(1784..1987)
                     /locus_tag="ACHOTW_004370"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFP9846351.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            2162..2338
                     /locus_tag="ACHOTW_004371"
     CDS             2162..2338
                     /locus_tag="ACHOTW_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846352.1"
                     /translation="MVNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIAT
                     EMCGISPGTVTCKR"
     gene            2899..3459
                     /locus_tag="ACHOTW_004372"
     CDS             2899..3459
                     /locus_tag="ACHOTW_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9846353.1"
                     /translation="MISVMILYVMSGSRLADNHTLSAGYAQSKVQDFKNIKGVNLQYR
                     YEWDSPVSVVGSFSYMKGDWADSHRDEADDFYRHQADIKYYSFLAGPAYRLNDYISFY
                     GLVGISHTKAKGDYEWRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNV
                     GYEGTKADIYGKHSVNGFTVGVGYRF"
     gene            3595..4386
                     /locus_tag="ACHOTW_004373"
                     /pseudo
     CDS             3595..4386
                     /locus_tag="ACHOTW_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(4391..5761)
                     /locus_tag="ACHOTW_004374"
                     /pseudo
     CDS             complement(4391..5761)
                     /locus_tag="ACHOTW_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5781..6128)
                     /gene="tnpB"
                     /locus_tag="ACHOTW_004375"
     CDS             complement(5781..6128)
                     /gene="tnpB"
                     /locus_tag="ACHOTW_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9846354.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(6125..6799)
                     /gene="tnpA"
                     /locus_tag="ACHOTW_004376"
     CDS             complement(6125..6799)
                     /gene="tnpA"
                     /locus_tag="ACHOTW_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP9846355.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT         1674 a         1649 c         1832 g         1894 t
ORIGIN      
        1 ttaaacgatt tatgaagaat tttactgttg tacagtacca gatactcagc cagtttcaga
       61 ttgaacatat tcagctattc aaagagcaat tcttcattaa actcaataaa ttgttccctc
      121 agtgttcggt taaaatgctc acttatggca tttattttcg gcgtatagag ataagtccat
      181 acgaacccga cggtggcttc ctgtagcgtt ttgtcgaatt ttcccgagaa ctctttgccc
      241 atcatcagtg atgatctggt tgataccaac aaggaacaac tgggctacac agcagaagaa
      301 atgcctgatg atatcacttt taagcgatag tacggccaga gagtagttgc tgtgttcgtc
      361 gagcatggta atgaaataac ggtacagctc gccattctga gctcaatagt atccatgcca
      421 ctgagcttac aggtctttat cgggcgatag tgctttattc ttcggggctt tgtggatgat
      481 ttttcgaaat gttcatgata gttaggtaaa gctaccgcat atcatttgca aaatgacaga
      541 tagatatact tcaatttgaa tcttaacaat tagtatggga atgctgtcaa tcctctgtat
      601 gcgtccacca catattgaaa tttttgttct ctaatatatt ccacgggcgt caagagtgtg
      661 aggcctgtgc tgttatacag caccagacgt tgtcccccat ctttgacctg tctcttgatc
      721 agatctcctg atcaagagac ttcatcacca ggtaaccctc agccatatcc tgatgtctga
      781 accagccatc ccacataact acccaaccgg ggctgcttgt gcgtttgctg tcatgccatc
      841 gccccagttt agccagtttc aaacaggccc atttcagggt cggtgtttgc gccggaagct
      901 gctttccttc cagcttaacc cacagtagtt tccactctgt cggcgtcagt attttctcac
      961 agctgtcatt ttgtgtttct tcactgatac ctccctgccg caggcccagc acccgcaccg
     1021 cgataaccac catgcgctca aggttatccc gggtctgcat tcgcagcgat tccacacatg
     1081 taccaccact tttccacgct ttgtggtatt cctctatcag ccagcgttgc tcgtaatggc
     1141 tgacgatacg tcgcgcatcg gcggcactcg ccactttttc tgatgtcagc ggataccagc
     1201 aggctccgtc ctctcctgct ccccgcaaca gacatacgtg agcgagagcg cctggccgct
     1261 gttgtcggga ttttttatgc tgacttcgct gtaactgatg agcatccggg cctggcgggc
     1321 tgcccgcccg cctttttgca tcacattcag cgtgtggctt cccgcggctg actttataat
     1381 gcaggtaatg ccagatatcc gcttcccggt cacagacagt gattacctgt ttctgtatct
     1441 ccctcagccg ttcggccatg cgctccgaag cctgctgcca gcggtaactt tctttttctt
     1501 cacagggacg ttcttttcgc tggtgcttaa caccgtaggt gtccgtggca cgactccagc
     1561 actgctgttc gataagatcg actggcaggg cgctgtcggg ggcgtacatc aggacagagt
     1621 gagccagcag cccgtgcgtc ttcgggttag tggtggtatt ccccaggtca tcaaatgccg
     1681 tactgtgacc gaagttaatg gtggtggtat cttccagcgc gaggagcagc ggatgtgcct
     1741 cacatgccct tgcagtggca gtaaatccgg cttcggcaat ggcttacggg gacaccgacg
     1801 ggttacgtat caggcggtac gcaccttcaa cctgagcagt gaagtgggat gatttcacaa
     1861 tagaaagacc tgcatgctga gcgagagaag aggtcagtga cacaaggcgt cgtgtacgac
     1921 gcggatcacc gagacgggca tgtccagact gctcgttagc cagtgaataa caatcagaaa
     1981 gtaccataac agacccgaag aaaatgaaat ataagagaag atcaacgggt gaagaaaaag
     2041 ttcaaaaaat cgctgccggg gaggaaggaa actaccggat ggaaagagtc cccctaaagc
     2101 agactgacag acatcacaaa tccccgggtg gggatttgtg tataagagac aggttacagg
     2161 tgtggtgaat aagactgcaa atgtggcagc tcatgccgtg gtgaatgctg cactggcggt
     2221 ggtacaggga aataacgccc gtgccagtgc cgcaggtgct gccactggag acgtggtggg
     2281 gatgatagcc acagagatgt gcggaatatc ccctggcact gttacatgta aaagataaat
     2341 aacgaagcta catccttctc tttatgaacg gctttatatt agttttgctg acaagattga
     2401 gctaaatccg gccaatgaag aagagcaggt tattctttaa aggtttgata acatgtaaca
     2461 cattttgaat attcagtcag taagccttaa aaatttacct ggttatgggc tgactgatat
     2521 aacaaagtaa atattcgatg gggaatgccg gatgcaatga acaatgcatt aacgtatcga
     2581 aataatgatg caggagcaaa ctcagtctgg tgagtataca ttatgccatt gatgcagagt
     2641 ttaaaggaac agggctggtt cgttatggaa caacatttgt gcctgaggaa agggggggga
     2701 gtatctgagg caacagaagt ttgtggggag ttcggtttat taccatgctt ggttttttgt
     2761 cagtgtgttc aaatcgtcag gtgatatatt tatagttttt tgatgatgat ttcaaataat
     2821 gtttttgaga tgtgtggtcc tttttgattt tcagaaatca tttattaatg aaggagggtt
     2881 aaatgaaaaa aactcgttat aatttcagta atgatattat atgttatgag tggttcaaga
     2941 ctggctgata atcatacgct atcggctggt tatgctcaga gtaaggtaca ggactttaag
     3001 aatattaaag gagtgaatct gcaatatcga tatgagtggg attctccggt cagtgtcgtc
     3061 gggagttttt cgtatatgaa aggtgattgg gcggacagtc atcgcgatga agctgacgat
     3121 ttttacagac atcaggctga tattaaatat tattcatttc tcgcaggccc agcttatcgt
     3181 ctgaatgact atatcagttt ttatggtttg gtggggatat ctcatactaa agcgaaggga
     3241 gattatgagt ggagaaacag tgtgggtgct gatgagtctg atggatattt aagtgagtct
     3301 gtatcaaaaa aatccactga ttttgcttat gcagctggtg ttattattaa tccgtggggc
     3361 aatatgtctg taaatgttgg gtatgaggga actaaagcag atatatatgg aaaacattcg
     3421 gtcaatggat ttactgttgg agttggttat cgtttttaat cattagacgg gactggataa
     3481 ttgcacttgc gtagtgaagt ttactaaatg tttgtatttt aatgaagtat tcgacatatt
     3541 cccttcagac ggaatatgtc gacggttatg gttttactgg agcatgagta tgttatggcg
     3601 aaactgataa atatatcagc gtccactgag gagagaatga cgccagggga gaggagggtg
     3661 gcttcacgac tggagagctt tctgaatgat gattgctttg tctggtacga tatcccggtg
     3721 ggacggaaaa accgacatcc ggattttgtc attattgacc cggataacgg gctggtattt
     3781 ctggaggtca aggactggac tgtatccaca ttacgtaaag caaatcagga gcaggttaca
     3841 ctggaaaccg acggtcttct gaaatcagaa ataaatccgc tggtacaggt tcgtcgttat
     3901 gcctgtgata cggttaatgc cttacctgct gacccgtgct tacggcagaa tgacggtcag
     3961 tataaggggc ggctgaacct ggcctgggcg tatggtgtgg tgtttacccg cattacccgt
     4021 cagcaactga aggcactgac agggaataat gagaatgcgg tggaaaagat ttttccttca
     4081 gcacagacaa tctgtcagga tgaaatgacg cagtctgttt tacctgaggt attccggcaa
     4141 aaaattgccg ggatgtttac cacggggttc agaacccggg taacaccccg gatgagggat
     4201 attctgcgcg cccatctgtt tcctgaagtg accgtgaaac agaactcaca aatcaaaatt
     4261 atggatattc agcaggagat tctggccaga aatattggtg acgggcatcg tgtcattcat
     4321 ggtgtggccg gttccggaaa aaccatgatt cttttgttcc gttgccttta cctggcagaa
     4381 accacgtccg gtagttacgc cgccccagtg caactacccg gagggcgttt tcacacaggt
     4441 tattgtcgat ctccacccag ccattgcggc agtattcgtt cagagcgtcc cattgcttca
     4501 gcagataggt gaacgctttc gctgtatccg agtggcgcga cagcgtgttc atctgcccct
     4561 ggagccactc atacaacgac tgcattagcg gtaccgttct ggcttttctg accgccagtc
     4621 gctcttctgc cggactgccg cggatctcag cctcgatagc gtacagttcc ccgatacgac
     4681 ggagcgcttc tcccgttact gtcgttggat ggcggacatg tacatcgtgg atcttgcgtc
     4741 gggcatgtgc catacaagcc gcttcggttg cctgaccgct ttcgtaaaga gcattgtaac
     4801 ccgcatatgc atcggcctgc aggatacctc tgtagtccgc cagatgttgc tgtgggtgga
     4861 tgcctttgcg gtcgggagag tatgagaacc acaccgctgc cggcatggtc gaaccggcgt
     4921 tgcgatcgtc acgaacatag acccacagcc gtccggtacg ggttttaccc tgacccggct
     4981 ccagtacatt taccggcgtg tcatcggcat gcactttacc cggcatcagc acatagtgct
     5041 tcagttcatc atacagcggg cgaagctgct ctcccatgat gtcaacccag cgccccatcg
     5101 tattgcggtg cagctccacg ccctggcggg catagatttc cgactgacgg tacagcggca
     5161 gatgctcggc gaacttagcc atgattatgc gggccagcag agccggactg gcgtaactgc
     5221 gctcgatggg ttttggtggc tgcggagcct gaactataca gtcgcaccgg ctgcaggccc
     5281 gttttgggcg aaccgtttcg attaccctga acgcggtgtt gatgatatcc agttgttcag
     5341 agatgctttc tcccagcggt ttcagtttac cgctgcagac ggggcattcg gtttctgccg
     5401 gggagataac ctgcctgtca cggggaagtg ttgccggaag tgctttgcgg acgggagagt
     5461 ctgatgtttt cggcgctgtc tctccggcca ttgaggtgag ttgcaactgc gcctcaccaa
     5521 gcctgttctg gagctcggtt atacgcgttt ctgcccgtgc gatcttcttt tctatcttct
     5581 cgcggctttt ctcgctgctg cgaccgaaca acattctctg cagtttagcg accagcgctc
     5641 tgagtgagct gatctcgcgg gcatagccgg ttatttcacc agacagacgg acgataacag
     5701 cctgctgtgc gatcagcagg gccttcagtt gctcgatgtt gtcggggagt gtgttgttca
     5761 ttcccctgtt ttatcacggg ttatatccgg atgccaggcc gttctgtccg tttgggatgt
     5821 tgccatgcga tcccctccag tagcatggat aactgagctg gcgtcaggtg cactttccct
     5881 tcccgggtta ccggccagac gaagcggccc cgttccaggc gtttggcgaa caggcataac
     5941 ccgtcacgat cggcccacag tattttcacc attttgccac tgcggccccg gaagacgaag
     6001 atatgcccgg agaacggggc atctttcagc gtgttctgca ccttcgaagc caggccgttg
     6061 aagccacaac gcatatctgt gatgccagcg atgatccaga ttctggtacc ggttggcagc
     6121 gttatcatcg ggtacctcct ttcatttcgc ggattagcgc ccgtaacatt tccggagtga
     6181 gagggtcaaa cagttttacc acacctgatt taagatgcag ctcgcaccgt gggacgtttc
     6241 cgggatcact ctcagggcac tcatcaggct tgttacgcca gaagggattt gtaactggtc
     6301 tggtcggctc tggcgtatca gtcagagcca ccgggacagg catgcattcc tgtatgtcat
     6361 catcgctcag taagccgtcc tcgtactggc ttttccattt taacagcagg ttatcattga
     6421 taccgtgctc tctggcgatc cgggcaacaa cagcaccggg ctgtaatgcc tgttttacca
     6481 gcctgatttt aaattcacga ctgtaggagg ttcgtcgttc ttttcgccat gtgccttcgc
     6541 tgatttgagg ctctgttaat tccttctttc tgttggcata aaggatggcg tcaagctgag
     6601 ctaatgaaac tgaatcgggc aatggccatg cgataccgga tgcaataaat cgctgaaaaa
     6661 gcgtatgtat tgtggaatga ctgagaccta gacgctgagc gatggcccgg atggtcagtt
     6721 tatcttcaaa tcttaaacgc agagcatcag gcaaataaga acggaagcag ggaatatctt
     6781 tttttgtctg ggaattcatc gttcgtgtcc atctatatag atgggcgcga ttgttgccag
     6841 acaggacaat tttcacaaga tgtcgcagat ggggcgctta cacaacaatg gcgcgaggga
     6901 aagctgctat taccttcttc agagagcccc cagctacttc ctgtgactct cgatgcagct
     6961 gccgaacagc cagaatcgct cgcagaggac ccggaaaccc tcagtatcag ctgttgtcaa
     7021 cgacggatga ctattgcacc gttcgtgac
//
LOCUS       DAZKIF010000193         7031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZKIF010000193 DAZKIF010000000
VERSION     DAZKIF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.194"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(182..667)
                     /gene="sixA"
                     /locus_tag="ACHOTW_004377"
     CDS             complement(182..667)
                     /gene="sixA"
                     /locus_tag="ACHOTW_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP9846356.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(870..3014)
                     /gene="fadJ"
                     /locus_tag="ACHOTW_004378"
     CDS             complement(870..3014)
                     /gene="fadJ"
                     /locus_tag="ACHOTW_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP9846357.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRAQLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3014..4324)
                     /gene="fadI"
                     /locus_tag="ACHOTW_004379"
     CDS             complement(3014..4324)
                     /gene="fadI"
                     /locus_tag="ACHOTW_004379"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP9846358.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4504..4788)
                     /gene="yfcZ"
                     /locus_tag="ACHOTW_004380"
     CDS             complement(4504..4788)
                     /gene="yfcZ"
                     /locus_tag="ACHOTW_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP9846359.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5160..6500
                     /gene="fadL"
                     /locus_tag="ACHOTW_004381"
     CDS             5160..6500
                     /gene="fadL"
                     /locus_tag="ACHOTW_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP9846360.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6865..>7031
                     /locus_tag="ACHOTW_004382"
     CDS             6865..>7031
                     /locus_tag="ACHOTW_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846361.1"
                     /translation="MLSSISINNTSAAYPESINENNNDEVNGLVQEFKSLFNGKEGIS
                     TCIKHLLELIK"
BASE COUNT         1795 a         1870 c         1640 g         1726 t
ORIGIN      
        1 gtaaaaatac attacaactt agtctcattt cgtcagaaat taatagggaa gcagaggaaa
       61 aagtgcgatg aattctgtat cgaagcgtat caactccgtg aagggttttg agtgaatttc
      121 tactattgca aaaagacaaa tgcccgatgt acagcttaca tcgggcattt tttcaaacga
      181 atcagatagc tttcgccatc ttcaaattac atgggctcat ctgccagttg aactcgcctt
      241 ttccgctttc atcaagcgtt acgctggcaa tggccgaggt ggtaaacata ggcggcgttt
      301 cgcccggaca taactcggcg accagatacc caactaacgg taagtgggag ataaccaaca
      361 ccgaagcaac accttcattg gtcaacgcct gcaaataggc actgaccaga ccaacatcgc
      421 cgcagggcgt taactccggc agaacttctg cactggaagg caggttcaga caatccccaa
      481 cttcctccag tgtttgctcg gctcgcagga acgggctcac cagaacgcgt tcgatttcca
      541 ctttttgacc tttcagccag ttcgccatca ggcgagattc gtcacaaccg ttagtggtca
      601 gaggacgaac cgaatcactg gcggcatcga gggctgcgtc gccgtgacgc atgataaaaa
      661 cttgcatatt gcaccgcttt tgttaaccag attcgcccgt tttctttcat ctgaaaccag
      721 agaaagaaaa cggtggccgg cattgtgcct tatccattca ccgaatgaaa cgctgttttt
      781 tacctcaatg gcgtaagtat agtcaatatg cgtttacatt ttgaccaaca atccgccatt
      841 cagcgcggat tcatatagct ttgaccttct tattgcaggt cagttgcagt tgttttccaa
      901 aaactttccc cacgcgcacc catctcgact aaacgctcgc aaggggtaaa acgtgaacca
      961 tactgcgtgg ccagtcgttg cattattgca accacttcgc ccgcgccgag agaatcgata
     1021 tagcgaaacg gtccaccgag aaatggcgga aaaccaatgc caaataccgc accaatatcc
     1081 ccgtcacgca cgctacggat aacctgctca tcaacacaac gtacagcttc attcagcatc
     1141 aacatcacac accgttcagc aacctgcggt gcggagagtc gcccctgccc ttgtgcgcca
     1201 atgagcgggt aaatggcagg atcgacctgt tttttgcttt tacgcccttt ctgaccataa
     1261 agatagaaac ctcgaccatt ttttctgcct ttgcgatcgt cgttcaaaat tgaagaaaca
     1321 acatttgcag gcgcgctaaa acgttctcca taagcggctt ccagtacagg aataatttta
     1381 gtcccggtgt cgattcctac ctcatccaaa agttggattg gacctaccgg aaaaccaaat
     1441 ttcactagcg cggcatcaat gtgcgctacc cgttcacctt cggtcagcat gcggatagct
     1501 tcattaatgt aaggcgctaa gatgcgattg acgtaaaacc cggctttgtc acgcacgaca
     1561 atgggcgttt tgccctgttt tttcgccagt tttaccgtag tggcgatggt ttgtgctgat
     1621 gtacccgcat gaggaataat ctccaccagc ggcatttttt ccaccggact gaagaaatgc
     1681 aggccgataa cttgctcagg tcgcgtggcg tgagcggcga tatcaccaat cggtaaagat
     1741 gacgtattcg aagcaaagat ggtatgagcg gcgcaatttt gctcaacttc cgccaccatc
     1801 tgttgtttca attcgagatt ttcaaacacc gcttcaataa tcagatcgcg atgggcaaag
     1861 ccgcgatagt ccgtcgttcc ggagattaat gccagctgtt tgtcacgttc gctggctttg
     1921 agatgacgac ggcgaacttt gccctccagc tgatcccaac tgtacttcag cgcatgattt
     1981 atgccctgcg ggttgatatc tttaattctg accggaatcc ccgctttaca agcagtgaca
     2041 taagcaatac cgccgcccat caagccacca cctaaaatcc ccacgctgtt taatggcgca
     2101 ggcggcgcat cactgccggg atctttcttc acttccgtac tggcaaaaaa gatactacgc
     2161 aaagcttgcg attgtggcgt catcgccagt tcgccaaacg cccgtgcttc agcgtcatag
     2221 ccactgctgg tgccctgcgc taatcccgtt tcaacaacct ccaggatgcg ttctgtcgcc
     2281 ggataattgc cttgagtttt gtgttctgtt ttcttgccga ccattttgaa cagctgcgca
     2341 cgacctaacg gccccgccag aatacgctcg cgtacaggta gagggcggga agatgggcga
     2401 tccttctttg ccagctcaac agcggcttcc agcagaatgg agtgcggaac aacatcatcc
     2461 accagcccca gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag gatcatctct
     2521 aatgctgtgc tgacgcctat cagacgcggt aaacgctggg tgccgcctga accgggtaac
     2581 aatccaagtt gtacttcagg caaaccgagc accgttttag gatcgtcagt acaaacgcga
     2641 ccgtggcacg ccagcgccaa ctccagccca ccacccaggc aagcgccatg aatagccgcg
     2701 ataaccggaa tgggcaaagc atgaatctcc gccatcaact gttggccctg ccgcgccaga
     2761 gcttccgctt cttgcgccgt tttgcaattg ccgatcatgt tgatgtctgc gccagcaatg
     2821 aagttgtccg gtttagcgga gacaaacacc acgcctcgca actctttgtt ttcacggagt
     2881 tgcttaataa tggcgcgcac ctgcgaggca aactccgcct tcagggtatt cattttctca
     2941 cccggtacgt cgatggtgat gatagcaatg ttgtccagac gaacattaag agtaaacgct
     3001 gatgtcattt ccattattcc gcctccagaa ccattgccgc gccaagccca ccggcagcac
     3061 aggcggtaac taaaccaaat ccaccgccgc gacggcgaag ttcatgcaat gtctgggtaa
     3121 tcatccgcgc gccggtcgcc gcgaagggat gcccgtaagc aatcgaaccg ccaagcacgt
     3181 taaatttgct atcgtccact tcgccagtgg catgtgcacg ccccagcgct tcacgagcaa
     3241 aacgttcact acccagtagc tgaatattgg ccaacgtctg ggcggcaaag gcttcgtgca
     3301 tatcaatcaa tgtcagatct gccattgtca aaccggcacg ctccagcgcc agcggtgttg
     3361 accaggctgg accgagcaac atgtcctgcc agacatcgat agcggtaaat gcgtagctgc
     3421 gcagatatcc cagcggtacc agccctaatt ctttcgcccg ggattcagtc atcaggatca
     3481 ccgccgctgc gccatcggtc agcggcgtac tgttcgccgc cgttaccgtt ccgtgtttac
     3541 gatcaaacgc cggacgcagc tttgcgaaat cagcaagcga ggaattaccg cgaatattgt
     3601 tgtcttccgc gagcagttgt ttataaggag ggataaaggc agtcatcacc tcttctttga
     3661 gttttccgtc tgaccatgcc tgagcggcac gctgatgcga acggtgcgct aatgcatctt
     3721 gctgttctcg ggtgatgccg taggttttcg ccatttgctc tgcagtgtcg cccatccgca
     3781 agccggtaga atattctgct accgcaggtg gtacgggcat taagtcgcgc aaacgcaggc
     3841 gagagaagag tttcagacgc tggctcattg tgcgggcttt gttgacatca accagcacgc
     3901 gcgccagttt tttactgacg ccaattggca ataccgaaga ggaatctgcc ccaccggcaa
     3961 tccccgctcg gatagtcccc gccatcaggc tttctacgac gtttgcaacc gcctggaaac
     4021 tggtagcgca agcgcggctg acgctgtaag catcggtatg cacattcatt ccagtaccga
     4081 gaacaatttc acgcgcaatg ttgggagctt caggcatttg tacgacctga ccaaagacca
     4141 gttgttcaat cacttcggcg gggatctcgc tgcgtgccag cagttcgcct accaccatct
     4201 tccctaaatc aaccgcggga atgccatgaa aagccgtcgc ctggcgggca aaaggcgtac
     4261 gtaaaccgct aacaatggcg atacgatcgc cctggcgggt aaccagcggt aaaacctgac
     4321 ccataacact cccctgtaaa aaaataaata aagtggtctg acctgatcat agtcttaacc
     4381 atttttttac atttagccaa gcggagaaaa gggaaattgg gagctatgac acagtgaaga
     4441 caggagaaag aaaagaaaac gcccctacct gttgctggca ggagcgtact tatccgagag
     4501 gaattaacgc agacccagct ggaagatcag catttcagct tcgcaggcaa aagtgaaatc
     4561 gatatccagg cgcacaccat cagactcttc agtgaaagtc ggggagattt tgcacggttc
     4621 ggattccacg ctacgggctt tctcagtcag cgccgccagc gtttgttctg cttctgcgcg
     4681 gtttgcaaac acgcggctgt aagacgctgt gcagtcggag ttgtccataa tggtgccaac
     4741 atccatacag cagcaaaccg gggtttcatc agcactacat ttactcatcg ttgatttcct
     4801 ctgtatgtgc acccaaggtg ccagataaac gttgtggata ttttacgctt ccggaaagtg
     4861 ctgctccagt tgttaattct gcaaaatcgg ataagtgacc gaaatcacac ttaaaaatga
     4921 tctaaaacaa aattcacccg aatccatgag tgcgccacct ccaaattttg ccagctggat
     4981 cgcgtttctt agatcatatt tgaaaaaaga tagaaacata cttgcaacat tccagctggt
     5041 ccgacctata ctctcgccac tggtctgatt tctaagatgt acctcagacc ctacacttcg
     5101 cgctcctgtt acagcacgta acatagtttg tataaaaata aatcattgag gttatggtca
     5161 tgagccagaa aaccctgttt acaaagtctg ctctcgcagt cgcagtggca cttatctcca
     5221 cccaggcctg gtcggcaggc tttcagttaa acgaattttc ttcctctggc ctgggccggg
     5281 cttattcagg ggaaggcgca attgccgatg atgcaggtaa cgtcagccgt aaccccgcat
     5341 tgattaccat gtttgaccgc ccgacatttt ctgcgggtgc ggtttatatt gacccggatg
     5401 taaatatcag cggaacgtct ccatctggtc gtagcctgaa agccgataac atcgcgccta
     5461 cggcatgggt tccgaacatg cactttgttg caccgattaa cgaccaattt ggttggggcg
     5521 cttctattac ctctaactat ggcctggcaa cagagtttaa cgatacttat gcaggcggct
     5581 ctgtcggggg tacaaccgac cttgaaacca tgaacctgaa cttaagcggt gcgtatcgct
     5641 taaataatgc atggagcttt ggtcttggtt tcaacgccgt ctacgctcgc gcgaaaattg
     5701 aacgtttcgc aggcgatctg gggcagctgg ttgctggtca gattatgcaa tctcctgccg
     5761 ggaagactcc tcaagggcaa gcattggcag ctaccgccaa cggtatcgac agtaatacca
     5821 aaatcgctca tctgaacggc aaccagtggg gctttggatg gaacgccggt atcctgtatg
     5881 aactggataa aaataaccgc tatgcactga cctaccgttc tgaagtgaaa attgacttca
     5941 aaggtaacta cagcagcgat cttaatcgtg tgtttaataa ctacggtttg ccaattccta
     6001 ccgccacagg tggcgcaacg caatcgggtt atctgacgct gaacctgcct gaaatgtggg
     6061 aagtgtcggg ttataaccgt gttgatccgc agtgggcgat tcactatagc ctggcttaca
     6121 ccagctggag tcagttccag cagctgaaag cgacctcaac cagtggcgac acgctgttcc
     6181 agaaacatga aggctttaaa gatgcttacc gcatcgcgtt gggtaccact tattactacg
     6241 atgataactg gaccttccgt accggtatcg cctttgatga cagcccagtt ccggcacaga
     6301 atcgttctat ctccattccg gaccaggacc gtttctggct gagtgcaggt acgacttacg
     6361 cgtttaataa agatgcttca gtcgacgttg gtgtttctta tatgcacggt cagagcgtga
     6421 aaattaacga aggcccatac cagttcgagt ctgaaggtaa agcctggctg ttcggtacta
     6481 actttaacta cgcgttctga taacgcgttc gcctggataa agtcacctgc tatgcaggtg
     6541 actttaactc ccccacttca ccgaaagtag tttcctcatt gcttgcctcc tgagttttgc
     6601 aaacagcctg atggcagcta gccctttcaa atcagtaagt ggtatccaca gacaaacctg
     6661 aagggaaacg catattttca ggcgttctgc tcgtccttct caaagagttt acttttctga
     6721 atttccagga tactcaaccc cctggctatt gtgcgctcat acacgcaaat taaagatagg
     6781 ttctaaataa atgagcgttt tttgatagtc tatttcatta ggtaatatat atttgtaaca
     6841 aatcaatcaa catggaataa aatcatgcta tcatctattt caatcaacaa taccagcgca
     6901 gcttacccag aatccatcaa tgaaaataac aatgatgaag ttaatggatt agtacaagag
     6961 ttcaaaagcc tttttaatgg taaggaagga ataagcacct gtattaaaca tctacttgag
     7021 cttataaaaa a
//
LOCUS       DAZKIF010000194         6997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZKIF010000194 DAZKIF010000000
VERSION     DAZKIF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.195"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..657)
                     /gene="yeeO"
                     /locus_tag="ACHOTW_004383"
     CDS             complement(<1..657)
                     /gene="yeeO"
                     /locus_tag="ACHOTW_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP9846362.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     P"
     gene            818..893
                     /locus_tag="ACHOTW_004384"
     tRNA            818..893
                     /locus_tag="ACHOTW_004384"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:851..853,aa:Asn,seq:gtt)
     gene            complement(931..1881)
                     /gene="cbl"
                     /locus_tag="ACHOTW_004385"
     CDS             complement(931..1881)
                     /gene="cbl"
                     /locus_tag="ACHOTW_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP9846363.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1983..2900)
                     /gene="nac"
                     /locus_tag="ACHOTW_004386"
     CDS             complement(1983..2900)
                     /gene="nac"
                     /locus_tag="ACHOTW_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP9846364.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3226..3301
                     /locus_tag="ACHOTW_004387"
     tRNA            3226..3301
                     /locus_tag="ACHOTW_004387"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3259..3261,aa:Asn,seq:gtt)
     gene            complement(3357..4292)
                     /gene="ldtA"
                     /locus_tag="ACHOTW_004388"
     CDS             complement(3357..4292)
                     /gene="ldtA"
                     /locus_tag="ACHOTW_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9846365.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4354..5433)
                     /gene="cobT"
                     /locus_tag="ACHOTW_004389"
     CDS             complement(4354..5433)
                     /gene="cobT"
                     /locus_tag="ACHOTW_004389"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP9846366.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(5445..6188)
                     /gene="cobS"
                     /locus_tag="ACHOTW_004390"
     CDS             complement(5445..6188)
                     /gene="cobS"
                     /locus_tag="ACHOTW_004390"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP9846367.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6185..6730)
                     /gene="cobU"
                     /locus_tag="ACHOTW_004391"
     CDS             complement(6185..6730)
                     /gene="cobU"
                     /locus_tag="ACHOTW_004391"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP9846368.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6882..>6997)
                     /locus_tag="ACHOTW_004392"
     CDS             complement(6882..>6997)
                     /locus_tag="ACHOTW_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFP9846369.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         1895 a         1887 c         1538 g         1677 t
ORIGIN      
        1 cggccaggag aaaaggccgt aaatcaatat gccgctaata ataatattaa gaatattcag
       61 gctaccgtta atcagtagcg gtattttcgt attccctgca ccacgaagtg ccccgctacc
      121 aattagagtg atggcagctg ctggataact gagtaccgtc agctccagat aagtcaacgc
      181 cagtgcttta acttctgtcg tggcatcacc cgcgacgaaa tcaataattt gctcgccaaa
      241 atgatgaata agcgttgcca acagtacggc aaacaacgtc atgatcacca atgactgccg
      301 cgtcgccacc ctcgctcgtc gtcgatcccg cttaccgaga ctaaatgcca caacgacagt
      361 agtaccaaga tcgatagcag caaaaaaagc cataatgacc atattgaagc tgtccgccaa
      421 tcccacgccg gccatcgcat cttttcccag ccagctgacc agaaaagtgc tcagaacccc
      481 catcaacagg acacaggcat tctccatgaa gataggaaca gcaaacgggg ttatctcgcg
      541 ccagaacaac actttgtagc tcttgcgttt agcgtgccag cgagtgccgt gaacaacctg
      601 gcgtaaagca gaggagatat tcaaagccga ccttaattgc agaaagtgaa accacatttc
      661 aaataatgag ggagaatcag caaagctgca aagattttcg ccatcaaatt gtctgcaaat
      721 gcaacaaact gttgatagaa acggcaaaca gttgggaaaa ttaaaaatcg ggtttgacaa
      781 aagatttttc gccgttaaga tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga
      841 acggcggact gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaatttct
      901 gaaaagcccg cttttatagc gggctttttg ctatatctga taatcaattt cctcttcact
      961 gttttccatc acctgccgct tgatatcctc aactgaaagt cctgcattgc aaagttccag
     1021 aaagcgccag acatagttac gctgaagttg tcctcgcttc agtcccaacc agacagtatt
     1081 agcatcgaaa agatgccgcg tatccaggcg gattaaattc ttttcctctt gttcaccgct
     1141 ggattgttcg gcaactaatc cgatcccaag cccaagagca acataggttt taatgacatc
     1201 agaatcctgc gcacttaata taatatctgc cagcaaacct ttgcgggcaa atgcgtcatc
     1261 aatacgtgag cgccccgtaa tcccctgtcg gtaagtgatt aacggccact tcgctattga
     1321 ttccagcgtc aatggtgtaa tttgcgtcaa gggatgatcg tgtggaacaa gcaaactatg
     1381 gtgccaacga aaccacggga aggcgacgag ctgcgggtca ttactcaaac gctcgctggc
     1441 gataccaata tcagcttcac cattatgcaa caacgtcgca atttcctgtg gcgtcccctg
     1501 gattagctcg agccgaacct ccgggaaaag ttcgcgaaaa gctttaatga cctctggcaa
     1561 gctataacgt gcctgagtat gcgtcgttgc aatagtgaga acgccagacg tatcgttggt
     1621 aaacaggtct gcaagccgac gaacattact ggcttcattg agaatacgtt ctgcaatgac
     1681 cagtaatgct ttgcccggtt cagtcatgcc cagcagtcgc ttacctcgtc gaacaaatat
     1741 ttcgatgcca agttcatcct ccagttcccg aatatgacgg ctgacgcctg actgtgaggt
     1801 aaaaagcata ttcgcaacct ctgtcaggtt gtaatcctga cgtgcagctt cgcggattat
     1861 ctttagttgt tggaaattca cggtaaactc cgggcagttc agatatcccg ttattgttaa
     1921 agtctaacgc ccggcataac aaataataaa aacccgcatc ttattccatc ccgatataac
     1981 gcttagctca ccaattgcca ctgccttttt tccatcactg gagaactaat cactgacatt
     2041 aacaactctt tcactgcctg tgcctgtggc gataagttcg ctctggcggg taaatttaat
     2101 gacaaagaga gactcatgga aggcgtggta atgcgtgaca tccacccatt tactgcgcca
     2161 cataacgaac gcgcggccga ttcgggtaat actgcaacgc ccatgccgct ggcaatcgct
     2221 gcggtaagcg tagcaataga ctcaatttca ccaatgactt ttgccgtgag ccgccgtagg
     2281 gaaaacgcct catcaacacg aagtctaaca gcgctgtaat cactggggag aaaaaggttc
     2341 atttgtgcaa tagcattaac atcaacgctt tgccccgggc aatcttgagt tcctaccagg
     2401 aaaagatctt ctttcagcaa agcctgactg gatacaccag ccacagggga atgctcataa
     2461 atcaccgcca tatcgagttg gtgatttatc agtttttcgt taagcactgc accgctattt
     2521 tcatgaagat agataacgac ctccgggaat tcagcgcgaa ccgcctgtaa taagggcatg
     2581 gtgatggatg acgcagcggt tcctggcgca aagccaatcg agacttgccc cgataatgcc
     2641 tgaccaacgt tatgcaccgc cagttgggcc tgctcacact gacgtaaaat ggcccgcgca
     2701 tgggtataga gaatttttcc ggcgtctgtt ggcgtaacgc cccgttttgt gcgaatcaaa
     2761 agttgttgat ttaactcacc ttcgagtgtg gcaacctgct ggctgagcgc tggttgtgcg
     2821 atatgcaata cttcagcagc ctgagtcagg ctaccaatat ctacaatttt tacgaagtat
     2881 ttcaggcgtc tgaagttcat gttgcctccg gtttttgaga atcggcccaa gtgccgccat
     2941 tacttacaac cagattgcaa gatgcttgcc agttttattt tggcgttgat gtacaagcta
     3001 accaactgtc aaataagaga ttatgataga ttcgtcattg gctcttttca ccagcagtcg
     3061 cgttcccctg ccctataaaa ggagggtatg caccacgatg gttcattacc aataagattg
     3121 aaatctcatc actttgttga aattgacagc aaacaaacaa aaaaatgcat ttcacccttt
     3181 gacatcacca tgcactgcca ttaatatgcg ccccgttcac acgattcctc tgtagttcag
     3241 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
     3301 aaattcaaaa aagcctgctt tctagcaggc tttttgcttt ctaattacca acgctcttaa
     3361 aacatctgtc ttgaaccaga actaatttgc acaggcattc ccgatcgacg ttgcaacgca
     3421 gcatttgcgc gattcacatc aacttcttgc ccgttgataa acgcccgcaa agatggggtt
     3481 accggcaatg gcacttttcg gtcagactca tattcagcac gattgcgcga cagcggctca
     3541 tgaacttcca gccactttga accatccggt tccgttgtgt atttcactgg ctgatcaata
     3601 atttgcacac gagtacctac aggaacatta tcaaacagat atttgatatc gtcattgcgc
     3661 agacgaatac agccctgact tacccggagc ccaataccaa aattggcatt ggtaccgtgg
     3721 atggcatata gcctgccaat ataaatcgcg tacagcccca tgggattatc gggccccgca
     3781 ggaacaaatg cgggcaaact ctcccctcgt ttcgcatatt cgcgccgggt gttcggcgtt
     3841 ggcgtccagg ttggtgcttc ttgtttacgt tcaacggtag tcacccagtt acgcggggtt
     3901 tctcgcccag cctggccgat accaatagga aagacttcca cagtattact gtctggtggg
     3961 tagtaataaa gacgcatctc agcgacgtta accacaatcc ctttacgaac agtgacgggc
     4021 aaaatcagtt gctgcggaat ggtgagttgc gaacctgact tcggtaagaa gacatcagcg
     4081 cccggattcg cttccagcat gttacttaac ccttgcccgt attgtgcggc aaaagtctcc
     4141 agcggctggg tattgtgatc aggaacagtt acagtaagcg actgccccac taaacggcta
     4201 ccctctggag gtaatggata agttaccgcc aggctagtat ggctggcaaa aagcagagca
     4261 aatgagcaaa gaatatttac acgacgcatc atgtcccttt cctaagtcgc gaaagctatc
     4321 ggttaagtat agcttttatc agacttttcg tttttaactg ttcaaatcag aagtcgtatt
     4381 ccccggtaga acaattttac tggcagcaag ttcgcccatg ttgttgtata tcgcacaagc
     4441 agcttcgatg atgggcatcg ccagagctgc gccactcccc tcacctaaac gcatatccat
     4501 attgagaaaa ggctccagcc ccaaatgcga cagcgctata cgcgcccctt tttctgccga
     4561 caggtgagaa ggaatgagat acggtttgat tgcaggagac atctggcagg ctgcgagcgc
     4621 agcagcataa gaaagaaatc catccagcaa cacgggtaaa ccgcaggaag cagcgcctaa
     4681 catcacgcca gccattccga ccaaatcaaa tccacccact tttgccagaa cattaacgcc
     4741 atcctgcgga tttggctgat tcaacgtaat cgcccggcgc acaacatcaa ttttattagc
     4801 cagtttatct gtcggcaggt ttgcgccaat cccaaccact tcttcaggat cccggccagt
     4861 gattgtgctg actattgctg ctgccggtgt cgtgtttgcc atccccagtt cacctacacc
     4921 aaacagcgtg acaccgtttt ttgccagctc ccgcgtataa catatgacgt ccaaaagtaa
     4981 cttttcagcc tgacggcgac tcattgccgg agctgaagca atattgccgc tacctcgtgc
     5041 gacacgcatg ttgataagcc cggggatagg ctcagcagta tcaataccaa catcaattac
     5101 gtggacgtta gcgcccgctt gtgctgccag cacacacacg ccggttgttc cgcgggtcat
     5161 attttcagcc tgaatggctg tcacttcttt tggggaaata gcgacccctt cctcccagac
     5221 gccgtgatcg gcacacataa ccagtaccgc ttttttgccc acatgcggta tgccattcaa
     5281 ccccggcatt cccgccagtt gtacgccaag cgcctccagc cttcccaggc taccaacagg
     5341 tttgagtaac ccgtcaatat gccgttgtgc acgcgacata gcggcaggat cgatggcagg
     5401 gatcgtattc agtaaattgg caagtgtttg catgtcacgt ctctttatag cagtgccagc
     5461 aggaagacca gttcaccaag ctctatggct gctcccagcg tatcgccggt ttgcccgccc
     5521 agcgtacgtt tgagaagctg accgaggatg aaaatcgcca ccatcgtcac caccatagca
     5581 gccacaccat gcatacccgg caataatacg gcagcaaaaa tagcggctaa gccgagtgtg
     5641 acgcaggttt gtcgcccatc aattttgcca atgaacacat tgccaagccc ttcttcccgg
     5701 gcataacgat gacgatacat caataatgcg gcagtcccgc ggctgaccgc acatgctgcc
     5761 gccagcgatg caaggatcgg ttcgccacgc agagccaact cgcttaacac caaaatcttt
     5821 gccagtacaa caaaaatcaa tgccagaccg ccgtgggtac ctaaacggct atcacgcatg
     5881 atttccaaca tgcgatcgcg gctacgtgca gaaaatacgc catcacaggt atcagcgagg
     5941 ccgtccagat ggaatccccc ggtcatcagc accagcacaa gtacgctaaa cagtgccgcc
     6001 agtggtgcgc cacaccatgc ctgcagcacc atgaagacca gcccgctaat cgcgccaagc
     6061 aataatccaa tcaaaggaaa agtaataata ccgcgagaat aatgctcgaa atccagtccc
     6121 tgggaccagc gacgcggtac aggcagacgc gtaataaatg agagcatcgc ccaaaataat
     6181 ttactcattt gatttttact ccaatacccg aaaccaccag ccatacttca tttgccgcag
     6241 cggccaactg ctgatttacc cgcccggcaa tatcacgaaa atgtcgtgcc agacgactct
     6301 ccggcacaat ccccattccc acttcgttag tcactaatac aacctttgcg gggcaacgtt
     6361 ggcaggcagc aatcaacgac tgaatctcag cattaatcgc ctgttccatc gcctgataat
     6421 cccattcatc agggtcttta tcgccgccat aatcaaacaa cagattagtc accattgtgg
     6481 taacgcattc aagcaacaca acctcattcg ggttaatgtc tgcatgaatt aattcatcaa
     6541 gatgttgcca gcgctccact gtgcgccagt gctccgggcg gccttgccga tgatgttcta
     6601 tccgtgcagc catctcatca tcaaggattt gcgaggtagc gatatacaga acctgtgaag
     6661 agtccccaat aagcgcctct gcgtggcgac tcttcccgct ccgtgcgccc cccgtcacca
     6721 aaatcatcat accgactcct gattcaaatg tatgatggta ttcattgaac agaacaatgg
     6781 atcccccacc gttgctgacc gcaaaacatt tacgcgatta atactgcgcg taatataatt
     6841 aaaaatccag cattctcaat ccattacgaa ataagatggc attacggaat agccagactt
     6901 tctcgtgtta acgtccgaca aaatgcgtca ttgacagcgg tttctgtgga ttgacacatt
     6961 tcattcagat aatgaattaa tgctactgcc ggaacaa
//
LOCUS       DAZKIF010000195         6970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZKIF010000195 DAZKIF010000000
VERSION     DAZKIF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.196"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            298..1335
                     /gene="dusA"
                     /locus_tag="ACHOTW_004393"
     CDS             298..1335
                     /gene="dusA"
                     /locus_tag="ACHOTW_004393"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP9846370.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1469..1711
                     /gene="pspG"
                     /locus_tag="ACHOTW_004394"
     CDS             1469..1711
                     /gene="pspG"
                     /locus_tag="ACHOTW_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP9846371.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1877..2860)
                     /locus_tag="ACHOTW_004395"
     CDS             complement(1877..2860)
                     /locus_tag="ACHOTW_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP9846372.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            2943..4358
                     /gene="dnaB"
                     /locus_tag="ACHOTW_004396"
     CDS             2943..4358
                     /gene="dnaB"
                     /locus_tag="ACHOTW_004396"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9846373.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4411..5490
                     /gene="alr"
                     /locus_tag="ACHOTW_004397"
     CDS             4411..5490
                     /gene="alr"
                     /locus_tag="ACHOTW_004397"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9846374.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5743..6936
                     /gene="tyrB"
                     /locus_tag="ACHOTW_004398"
     CDS             5743..6936
                     /gene="tyrB"
                     /locus_tag="ACHOTW_004398"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP9846375.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1631 a         1724 c         1869 g         1746 t
ORIGIN      
        1 ttttttctct atcagtgcta ttagcaggga gagatatcac cggagtttaa tgtgtgattt
       61 ttatttatcg tcgaatctgt attgtttatc attgcttttc acaacgctaa catctaataa
      121 gattagatat tcacttcgtg agagcttcat gccttgagag gatctcaatt ttcttttgca
      181 atgagacagg cgcttcctgt tgttatggta tagtaccccg ttattgagcc tcctgaacag
      241 tgatgctgaa taacataacc acatgatata tcgataaaat aatctctaca tttgaaaatg
      301 cacggtaatt ctgaaatgca aaaaatcaac caaaccagcg caatgcctga aaaaactgac
      361 gttcactgga gtggtcggtt tagcgttgca ccaatgctcg actggacgga cagacattgc
      421 cgctatttct tgcgtctgct ttcccgcaat acgttgctgt ataccgaaat ggtgactaca
      481 ggggcgatta ttcacggtaa aggtgattac ctggcgtaca gtgaagaaga acatccggta
      541 gcgttgcaac tcggcggtag cgatccggcg gcgctggcgc agtgtgcgaa gctggcagaa
      601 gcgcgtggat atgatgagat caacctgaat gtcggctgcc cgtctgaccg ggtgcagaac
      661 ggcatgtttg gtgcgtgtct gatgggtaat gcgcagctgg ttgccgactg cgtgaaagcg
      721 atgcgcgatg tggtgtcgat tccggtgacg gtgaaaacgc gtattggcat cgatgaccag
      781 gacagctatg aatttctctg cgatttcatc aacaccgttt ccggcaaagg cgagtgtgag
      841 atgttcatca tccacgcacg taaagcctgg ctttcggggt taagcccgaa agaaaaccgt
      901 gaaatcccgc cgctcgatta tccgcgtgtg tatcaactga agcgtgactt tccgcatctg
      961 acgatgtcga ttaacggtgg tatcaagtcg ctggaagagg ctaaagcgca tttgcaacat
     1021 atggatggcg tgatggtcgg gcgcgaagcg tatcagaatc cgggtattct ggcggcggta
     1081 gaccgagaga tctttggttc ctcggatacc gatgccgatc cggtggcggt agtgcgcgcc
     1141 atgtatccgt acattgagcg tgaactcagc caggggacgt atcttggcca tattacccgg
     1201 catatgttgg gcttgttcca gggtattcct ggcgcgcggc agtggcggcg ttatttaagt
     1261 gaaaatgccc ataaagcggg tgcagacatt aacgtgctgg aacacgcgct caaactggtg
     1321 gcggataagc gttaactttt caccaaaaag tagtcaaatt caccacgccc tgcgcaccgt
     1381 cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt gtaatgcctg
     1441 caagagattt catatttggg agagcatcat gctggaacta ctttttgtga ttggcttttt
     1501 tgtcatgttg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc tggttgtggc
     1561 gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc tgccgtggtt
     1621 actcctggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa aagtgccgaa
     1681 atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca caacctaagg
     1741 ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg cgcctctaac
     1801 agattcatcg tgctgtaccc tacatacagc cgaactataa aaagaaaggg cttcccaggt
     1861 ggaagcccta tttcttttat ggaatcaaca ggctggaacc ttgcgtcgcg cggctttcca
     1921 gaatctcatg cgcacgctgc gcatccttca gcggatattt ctgctgctcg gcgacatcga
     1981 ccttaatcac accgctggca atcaaagaga acagttcatt actggcctcg gttaattcct
     2041 cccgcgtggt gatatagcct tgcagggaag ggcgtgtcac atacaacgag cctttctgat
     2101 tgagaatgcc taagttcaca ccggtaaccg cacctgatga gttgccaaaa ctcaccatta
     2161 agccgcggcg ttgcaggcaa tccagcgacc gttcccaggt gtctctgccc acggaatcgt
     2221 acactacgcg cactttttta ccaccggtga tctcttttaa ccgctcgacc agattctctt
     2281 cacgatagtt aataacctgc cacgcgcccg cttttagcgc gctctgcgct ttttgcgcgg
     2341 ttcctacggt gccgataagt ttcgcgccca gggcttttgc ccactggcag gcaatcaagc
     2401 caacgccgcc agctgctgcg tggaacagga actgttcatc gggtttaatt tcataggttt
     2461 tgcgcagcag ataataaacc gttaagcctt tcaggaaaga tgctgcagct tgctcaaaag
     2521 aaattgccgc aggcagaatc gccgctttat ccgcattaat gttatgcaca gagctgtaag
     2581 cgcctaacgc cgactgcgca tagactacac gatcgcctgc cttaatatgc tttacaccac
     2641 tgccgacttt actcacgatg cctgccgctt cggtacctaa tccgctgggt agtgatggcg
     2701 gcgggtaaag gccgctgcgg atatacgtgt cgataaaatt gatgccgatg gctttatttt
     2761 cgacctggat ttcattctcc gccggatcgg caggagtgaa ctctacggct tgaagtactt
     2821 ccgggccacc gtgcttgtga aattcaattc gtgttgccat gtgtcctcca gaacgtatcg
     2881 tcagggtctg cttcatatgg taaagtttcg acccattctt tatctcggta actccattca
     2941 ctatggcagg aaataaaccc ttcaacaaac agcaggctga accccgcgaa cgcgatccac
     3001 aagttgccgg gctgaaagtg cctccgcact cgatcgaagc ggagcagtcg gtgttgggcg
     3061 gtttaatgct ggataacgaa cgctgggatg atgtagccga gcgtgtggta gcagacgatt
     3121 tttacacccg cccacaccgt catatcttta ctgaaatggc gcgtttgcag gaaagcggta
     3181 gtcctatcga tctgattacc cttgcggaat cgctggaacg ccaggggcaa ctcgatagcg
     3241 tcggtggttt tgcttatctg gcagagttgt caaaaaatac gccaagtgct gcgaacatca
     3301 gttcctatgc ggacatcgtg cgtgaacgtg ccgttgtccg tgagatgatc tcggttgcga
     3361 atgagattgc cgaagctggt tttgatccgc aggggcgtac cagcgaagat ctgctggatc
     3421 tggctgaatc ccgcgtcttt aaaattgccg aaagtcgtgc gaacaaagac gaagggccga
     3481 agaacatcgc cgatgtgctc gacgcaaccg tggcgcgtat tgagcagtta tttcagcagc
     3541 cacacgatgg cgttaccggg gtaaacaccg gttatgatga tctcaacaaa aaaaccgctg
     3601 gcttgcagcc gtcggatttg atcatcgtcg ccgcgcgtcc gtcgatgggt aaaacaacat
     3661 ttgcgatgaa cctcgtcgaa aacgcggcga tgttgcagga taaaccggta cttattttct
     3721 cgctggagat gccatcagaa cagatcatga tgcgttctct ggcgtcgctg tcgcgcgttg
     3781 accagactaa aatccgtacc gggcagctcg atgatgaaga ctgggcgcgc atttccggca
     3841 ccatgggtat tttgctcgaa aaacgcaata tctatatcga tgactcctcc ggcctgacgc
     3901 caacggaagt gcgttcccgc gcacgccgta ttgcccgtga acacggcggc atcgggctta
     3961 tcatgatcga ctacctgcaa ctgatgcgcg taccggcact ttccgataac cgtacgctgg
     4021 aaattgcaga aatctcccgc tcgctgaaag cactggcgaa agaactgaac gtgccggtgg
     4081 tggcgctgtc ccagttgaac cgttctctgg aacaacgtgc cgacaaacgt ccggtcaact
     4141 ccgacctgcg tgaatctggc tctatcgagc aggatgcgga cttgatcatg tttatctatc
     4201 gtgatgaggt gtatcacgaa aacagtgatt taaaaggcat cgcggaaatt attatcggta
     4261 aacaacgtaa cggcccaatc gggacggtac gcctgacctt taacggtcaa tggtcgcgct
     4321 tcgacaacta tgcggggccg cagtacgacg acgaataata attattttat gaattaggta
     4381 attaaagcaa acacttatca aggaacacaa atgcaagcgg caactgttgt gattaaccgc
     4441 cgcgctctgc gacacaacct gcaacgtctt cgtgaactgg cacctgccag taaaatggtt
     4501 gcggtggtga aagcgaacgc ttatggtcac ggtcttcttg agaccgcgcg aacgctcccc
     4561 gatgctgacg cctttggcgt agcccgtctc gaagaagctc tgcgactgcg tgcgggggga
     4621 atcaccaaac ctgtactgtt actcgaaggc ttttttgatg ccagagatct gccgacgatt
     4681 tctgcgcaac attttcatac cgccgtgcat aacgaagaac agctggctgc gctggaagag
     4741 gctagcctgg acgagccggt taccgtctgg atgaaactcg ataccggtat gcaccgtctg
     4801 ggcgtaaggc cggaacaggc tgaggcgttt tatcatcgcc tgacccagtg caaaaacgtt
     4861 cggcagccgg tgaatatcgt cagccatttt gcgcgcgcgg atgaaccaaa atgtggcgca
     4921 accgagaaac aactcgctat ctttaatacc ttttgcgaag gcaaaccagg tcaacgttcc
     4981 attgccgcgt cgggtggcat tctgctgtgg ccacagtcgc attttgactg ggtgcgcccg
     5041 ggcatcattc tttatggcgt ctcgccgctg gaagatcgct ccaccggtgc cgattttggc
     5101 tgtcagccag tgatgtcact aacctccagc ctgattgccg tgcgtgagca taaagtcgga
     5161 gagcctgtcg gttatggtgg aacctggata agcgaacgtg atactcgtct tggtgtagtt
     5221 gcgatgggct atggcgatgg ttatccgcgc gccgcgccgt ccggtacgcc agtgctggtg
     5281 aacggtcgcg aagtgccgat tgtcgggcgc gtggcgatgg atatgatctg cgtagactta
     5341 ggtccacagg cacaggataa agccggggac ccggtcattt tgtggggtga aggtttgccc
     5401 gtagaacgta tcgctgaaat gacgaaagta agcgcttacg aacttattac gcgcctgact
     5461 tcaagggtcg cgatgaaata cgtggattaa tcgttctgta atatttgatt gtctgtgccg
     5521 gatgcggcgt gaatgcctta tccggccaat aaaatcctac caattcaata agttgatatt
     5581 ctttcatgct cttataaagg tcgtgcctct ggcggatgta cgtttgtcat gagtctcact
     5641 ctgttgctaa ttgccgttcg cccctgaaca tccactcgat cttcgccttc ttccggttta
     5701 ttgtgtttta accacctgcc cgtaaacctg gagaaccatc gcgtgtttca aaaagttgac
     5761 gcctacgctg gcgacccgat tcttacgctt atggagcgtt ttaaagaaga ccctcgcagc
     5821 gacaaagtga atttaagtat cggtctgtac tacaacgaag acggaattat tccacaattg
     5881 aaagccgtgg cggaggcgga agcgcgcctg aatgcggtgc ctcatggcgc ttcgctttat
     5941 ttaccgatgg aagggcttaa cagctatcgc catgccattg cgccgctgct gtttggtgcc
     6001 gaccatccgg tactgcaaca acagcgcgta gcaaccattc aaacccttgg cggctcaggg
     6061 gcattgaaag tgggcgcgga tttcctgaaa cgctacttcc cggaatcagg cgtctgggtc
     6121 agcgatccta cctgggaaaa ccacgtagca atattcgccg gggctggatt cgaagtaagc
     6181 acttacccct ggtatgacga agcgactaac ggcgtgcgct ttaatgacct gttggcgatg
     6241 ctgaaaacat tacctgcccg cagtattgtg ttgctgcatc catgttgcca caacccaacg
     6301 ggtgccgatc tcactaatga ccagtgggat gcggtgattg aaattctcaa agcccgcgag
     6361 cttatcccat tccttgatat tgcctatcaa ggatttggtg ccggtatgga agaggatgcc
     6421 tacgccattc gcgccattgc cagcgctgga ttacccgctc tggtgagcaa ttcgttctcg
     6481 aaaattttct ccctttacgg cgagcgcgtc ggcggacttt ctgttctgtg tgaagatgcc
     6541 gaagctgcag gccgcgtact ggggcaattg aaagcaacag ttcgccgcaa ctactccagc
     6601 ccgccgaatt ttggtgcgca ggtggtggct gcagtgctga atgacgaggc attgaaagcc
     6661 agctggctgg cggaagtaga agagatgcgt actcgcattc tggcaatgcg tcaggaactg
     6721 gtgaaggtat taagcacaga gatgccagaa cgcaatttcg attatctgct taatcagcgc
     6781 ggcatgttca gttataccgg tttaagtgcc gctcaggttg accgactacg tgaagaattt
     6841 ggtgtctatc tcatcgccag cggtcgcatg tgtgtcgccg ggttaaatac ggcaaatgtg
     6901 caacgtgtgg caaaggcgtt tgctgcggtg atgtaatgca ggaaagcagg ctggaattac
     6961 ccagcctgca
//
LOCUS       DAZKIF010000196         6857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZKIF010000196 DAZKIF010000000
VERSION     DAZKIF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.197"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            35..837
                     /gene="ydjO"
                     /locus_tag="ACHOTW_004399"
                     /pseudo
     CDS             35..837
                     /gene="ydjO"
                     /locus_tag="ACHOTW_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(841..2232)
                     /gene="tcyP"
                     /locus_tag="ACHOTW_004400"
     CDS             complement(841..2232)
                     /gene="tcyP"
                     /locus_tag="ACHOTW_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP9846376.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESIYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(2365..2955)
                     /gene="ydjM"
                     /locus_tag="ACHOTW_004401"
     CDS             complement(2365..2955)
                     /gene="ydjM"
                     /locus_tag="ACHOTW_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9846377.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRPIKHQVEY"
     gene            complement(3118..3786)
                     /gene="hxpB"
                     /locus_tag="ACHOTW_004402"
     CDS             complement(3118..3786)
                     /gene="hxpB"
                     /locus_tag="ACHOTW_004402"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP9846378.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3933..4469
                     /gene="yniB"
                     /locus_tag="ACHOTW_004403"
     CDS             3933..4469
                     /gene="yniB"
                     /locus_tag="ACHOTW_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP9846379.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4510..5370)
                     /gene="yniA"
                     /locus_tag="ACHOTW_004404"
     CDS             complement(4510..5370)
                     /gene="yniA"
                     /locus_tag="ACHOTW_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9846380.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5476..5766)
                     /gene="ghoS"
                     /locus_tag="ACHOTW_004405"
     CDS             complement(5476..5766)
                     /gene="ghoS"
                     /locus_tag="ACHOTW_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9846381.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5867..6796)
                     /gene="pfkB"
                     /locus_tag="ACHOTW_004406"
     CDS             complement(5867..6796)
                     /gene="pfkB"
                     /locus_tag="ACHOTW_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP9846382.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1819 a         1658 c         1650 g         1730 t
ORIGIN      
        1 agataataat tttgttaaca atatgaggcg ggagatgttt tctacaaact taatacagag
       61 taattatggt gatttaaata ttaaaagcct ggctttcgac tcttttaaag aaaggctgca
      121 gtctactatg acagcactca ctttctttat atcaacgggt caatgtgact gcgatgaaat
      181 agcagaatca aactttaatt atatgattgc ctatatgtct aacattaact atgatgcctc
      241 aaagccagga gcgccagcac ttagttttga tacatatctg caggacaatg ttaaatatag
      301 agtaataatt aataatcttt atggtagtga aattcggata agaggtataa ataaagattt
      361 tattggaatg gatgtcactt ctgtatttcg cccagaaaaa atgaccaatt tgatcagtat
      421 aaaaaacagg ttggttattc actatttaag gactatatac tatgaacaat attatataca
      481 tcctgtcggg agcatttttg ctgcgattca aaaaaatgag agtctcctaa agtttccttc
      541 gataatatca atgcttaata tcaatcttct ttttaatcca ctaaatcttc cgggaatggg
      601 aagtggtata cttgaagata ttatgagtat tccagatagt tcacttagaa aacgtctcgg
      661 gtatgaggtt ttaagcttct cgctacaggc acattcattg agccaggaat gtattgataa
      721 gttggacatt ttttcgctga cgatctgttt aaatatgagt cagtatgcat cgccgcgatg
      781 gaacatttaa aaagtaaggc aacggcccct ttacaaaacg gaccgttgcc agcataagaa
      841 ttaatggtgt gccagttcgg cgtcgtcttc gctatccaga atggctttat cggtttgctt
      901 cagccactgg ctggtcagcg tgccagctgt catcgagcca ctaacgttta gcgccgtacg
      961 gcccatgtcg ataagcggtt caacggagat taacagcgcc accagggtta ctggcaggcc
     1021 catcgcaggc agcacaatca gtgcggcgaa agttgcacca ccaccgacac cggcaacgcc
     1081 tgcggagcta acggtaacaa taccgaccag cgtcgcaatc cacatcgggt ccagcgggtt
     1141 aatgccaacc gtaggcgcaa ccatcaccgc cagcattgcc ggatacaaac cggcgcagcc
     1201 gttctgacca atggtcgcac cgaaagaggc ggcgaaactg gcgatggatt caggaacacc
     1261 gagtcgacgc gtttgtgctt ccacattcag tgggatagac gcagcactgg aacggctggt
     1321 aaaggcaaac gtcagcacag gccatacctt acggaagtac ttcagcggac tcacgccatt
     1381 aatgcccagc agaatgccat gcactgcaaa cataatcagc agaccgaggt aggacgcgac
     1441 aacgaaactt cccagtttga tgatgtcttg caggttagaa cctgcaacca ctttggtcat
     1501 tagtgccaga acgccgtaag gggtcaactg catgaccagg cgaactagtt tcatcaccca
     1561 gctttgcagg gtatcgatag cggttaagac gcgttcacct ttcggcgcat catccttcag
     1621 cagtttcagc gcagctacac cgaggaatgc ggcaaaaatt accacgctga taattgacgt
     1681 cggattggct ccggtcagat cggcaaacgg gtttttcggg ataaaggaca agaccagctg
     1741 cggaacgctc aggtcagaga ctttaccaac atagatactt tcgatggcgt tcagacgtgc
     1801 agtttctgca ccaccctgaa ccagaccttc agccgtcaaa ccaaacaggt tggttaccag
     1861 cacaccgacc agcgccgcaa tcagcgtggt aaacaaaagc gtaccaatgg tcagaaaact
     1921 gattttgcct aactgagatg cgttatgcag acgggcaacc gcgctcagaa tagaggcgaa
     1981 gactaacggc ataacgatca tttgcagcag ttgaacatag ccgttaccaa caatgttaaa
     2041 ccactgtaca gaatctttaa gtacctggct gtcagaacca taaatggtat gcagggcaag
     2101 gccaaaaacc acacccatca ccagacccac cagcactttt ttcgccagac tccactgttt
     2161 atggcgggtc tgagccagcg caaacagcag tacaacgaac accacgatgt tcgcaattaa
     2221 tggaaagttc atccccgttc tcctgatctt attatttcta tcactgcgta gcagtcatag
     2281 tgttggcgca agattaacag aactgtgagc tggctcttat attcaaatgg aattgcttat
     2341 tacagaaagg cgatttttgc ctttttagta ttcaacctga tgcttaatag gccgatgaaa
     2401 ctggatctgc aaggtattga tcatttgcga tgaccaacga acagcgctgt tttcgggtaa
     2461 tgaatgaggc atccataccg accagacaaa taatgccatg cagataaaac gttccagttg
     2521 gttacccttt tggggaacca ggataggcaa gcggaaacgc cagcggcatg gccagagcag
     2581 gggaacaccg gcgggtgtca gcatatcggc aagtatgtgg cttaaataac ccagcaccat
     2641 tccttgtagc gcatcagccg gaatgaacca gctttcagga accttaaggt aaaaggttgc
     2701 cagcagcgca aataccgcca gcagactgtg ggtaaaacca cgatgcccaa aagcgcgggc
     2761 gatcggtttt gatatccact ttaatcgctg cccaagaaac gactttgggt gatcgatgtc
     2821 cggtaacaaa cacgtcagga ttgcggaagg gacaatatgc caccagtcac cctgtgccag
     2881 cacgggcgtc agctcggcat ttttggcaaa taccgcacaa gcaatagaaa agagaaggtg
     2941 accttccgcc gtcatgataa aactcactaa actgtcgata cgtacagtat aggattttta
     3001 tacagtgcac ggaagtggtg acggtgtaac tctttctctt aaattgacga ttaatccgtc
     3061 agttgaatgc aaattcagat aaagaaccag gggcagcgtg atcactgccc ctggtcatta
     3121 accgagaagg tcttttgcgg tgagttctgt cagcgatgag agtttgacgt ttgctaatac
     3181 aaaacgtgga tcattttgcg cttctggcgc aggaacgacg atggaacgca tgcgggctgc
     3241 tttagaggcg atcatgccat ttaccgaatc ctccagcgct acgcaggtca gtgggtcaac
     3301 gcccagtttt gctgcgcagt cgagatatac ttgcggatgc ggcttgctgt aaggcagttt
     3361 ttcggccgag gcgagggcat cgaaactgtc gcgtaagtca aacatggtca acactttttc
     3421 cagcatatgt agtggtgacg cggaggccag tcccaccaat aaaccttgtt ctttgcataa
     3481 cgcaacggct tcgcgcacgc ctggtaataa tggacgtgtc tcttcaacca gtgaaatggc
     3541 acgggcaata acccgttcta ctacttcctg acggcttggc ccattccacg gttgccgggc
     3601 gtaccaaaga tcgaccacca tatcgatgcg taaacctaag gtgtccggca gctcgttacg
     3661 acgggagata tccaccccca ggcttgccat cacatccagt tcggctcgat cccataaagg
     3721 ttctgagtcg ataagtaatc catccatatc aaaaattgca gcaagaatct gacgcggggt
     3781 tgacattaca acctctcctt tagagcatta tgtgtaaggt attaagccgt gcttatgatt
     3841 aagcgcctat catatcgcct ttaaagaatg ggcgaaaacg gatatctaca ggtaaactaa
     3901 gtgaaacgac gcgtcttaat caaggagaac tcatgacgta tcaacaagct ggacgcattg
     3961 ctgttttgaa aaggattatg ggttgggtga tttttatacc tgcattgatc tctacattga
     4021 tttctttgct gaaatttatg aatacccggc aggaaaacca ggaaggcatt aatgcggtca
     4081 tgctcgactt tactcatgtc atgatcgata tgatgcaggc gaatacgcct tttttgaatc
     4141 tgttctggta taactccccg acgcccaatt ttaatggtgg cgtgaacgtt atgttctggg
     4201 tgattttcat cctgattttt gttggactgg cgttgcagga ttccggtacc cggatgagtc
     4261 gccaggcgcg cttcttgcgg gaaggtgttg aagatcaact cattctggaa aaagccaaag
     4321 gggaagaggg gctaacgcgt gaacaaattg agtcacgtat tgttgttccg caccatacta
     4381 ttttcctgca gtttttctca ctgtatatcc tgccggttat ttgtattgct gcaggttatg
     4441 tgttcttttc tctgcttggg tttatttaat caaaaggccg cacggagcgg ccattatctt
     4501 caacccatat cacgcagcta ataatctatc cagtgactgc tgagcaataa ccaaatgctg
     4561 accgccaaat aatcttgcac gatttaacag cgtgtagagt tggtaaaccg gttgacgttc
     4621 aaggaaatcg gcaggtagcg gggatactga ctgatagccg tcatagattt gtggcggctg
     4681 ttcagtatgc agcggtaaca tcgccaggtc gcactctcgg tcaccccagt agcaggccgg
     4741 gtcgaaaatg tacgggccat ccggacccag cgcacagttg ccggaccata aatcgccgtg
     4801 caacagagaa ggctgcggtt gatgtgaggc gagacgctgc tgaatatgct cgacgatggc
     4861 atcgatattg ccgaaagcga tccctttttc tgctgccagt tccaactgcc agccaatccg
     4921 ttgttcagca aaaaacgttg accagcgacg ttgccaggtg ttgggctgtg gagttgtgga
     4981 gagcgagtta tcaaaatcga gtccaaattg tggttggtcg ctccattgat gtaaacgcgc
     5041 aatttgctga ccaagaataa atgcgctatg cgcatccagc ggacgaggtg ggagataatc
     5101 catcaccaga aaactgtagt cacggtcagc gccaactgcc caaaccttag gcacggtgac
     5161 ggttttacta cgcgacagta actccagttg gtcggcttcg gcggtaaaac cgggaagcag
     5221 ttccctttca tcacatttga cgaaaaagtc atgtcctgca tagcgcaaat gccatgcggc
     5281 atggacttct ccgccaggca gttcattacg cagttcgatt tcgccttcac ctaactgctc
     5341 gctcaaaaga cgactgattg cctgccacat gatgtctctc ccatgttgtc tgccagatca
     5401 taaggttagc gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac agactgatta
     5461 ttgcccaaaa accatttatt ttctgttgct attccattcc tcccaggtcg tgatggtgat
     5521 ttcaggatct ttgccggttg cactttgggt aagccccgaa accagttctc taatttcttc
     5581 ttcactttgg gtactgataa gtccaaaagt gttagtcccc agctcatgga tatttccctc
     5641 atcatccgtc atggtgagca aaaaaccgtc gcgagtcagg taattattca actcgttaat
     5701 ttcagtcaat gtatcctcat gcaacatgac ggttatgaca taacggacaa gatcgccact
     5761 ggccatagtt cacccctttg ttatcacatg acttttgagc atagtcggag aaacgcgttg
     5821 ccgacaggtt ggtgatgatt cccccaatgc tgggggaatg tttttgttag cgggaaaggt
     5881 aagcgtaaat tttttgcgta tcgtcatggg agcacagacg tgttccctga ttgagtgtgg
     5941 ctgcactccc cgcagctacg ccaaaacgaa ccatctcttc aagagaggca ttttctgcca
     6001 gtttcagtgt catcgcgccg accatgctat caccagcgcc aacggtactc tggcttttca
     6061 ccggtggtgg caccacctga atgcagtttt cactatcaac acccagcgct ccttgtggac
     6121 ccagggaaac gacaacccgt ttggccttgc cgctattaac gatttcctgc gcggctttgc
     6181 ggacatcgtc tggctgggtg agttcgcgat tcaccagcgc actgagttct ttttggttag
     6241 gcttaaccaa ctcgatgtta ccaatcgcca gtgctgcact taacgcttcg ccagaactgt
     6301 cgatgatgca gcggatccct tggttttgcg cagcggaaat cagttgggtt aatttttcca
     6361 gcttcacacc tggcggcagg cttccgctta tgaccaggat ggccccggat tcaatttcca
     6421 gaacttgctc ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct ggcataacaa
     6481 aacgatactg ctcaccgctt gcttccacat gtacgtgtaa attctgccgg gtccagtctt
     6541 tggcttctac agtagcgacg gggacatttt catccgccaa cagtgaaacc aggtgttcgc
     6601 cggtcgcgcc acccgccggg aagatcgctg tggcactgcc tccaagatgg gcaatggcgc
     6661 gggcgacgtt gatgccgccg cccccgggtt cgaacaccgg tgcggtacag cgcagttttc
     6721 cttcgggata aatttgcggg gtaattgttg cgctatcgag agagggcgca agtgtcaacg
     6781 tatagatacg taccatcatt tcctcctata ggctgatttc agtctggcac cgaatcagcg
     6841 gaaagtgaag aaattaa
//
LOCUS       DAZKIF010000197         6761 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZKIF010000197 DAZKIF010000000
VERSION     DAZKIF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.198"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..134)
                     /locus_tag="ACHOTW_004407"
     CDS             complement(<1..134)
                     /locus_tag="ACHOTW_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846383.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     "
     gene            463..1335
                     /locus_tag="ACHOTW_004408"
     CDS             463..1335
                     /locus_tag="ACHOTW_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9846384.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVWGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1707..4556
                     /gene="cah"
                     /locus_tag="ACHOTW_004409"
     CDS             1707..4556
                     /gene="cah"
                     /locus_tag="ACHOTW_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFP9846385.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            4667..>6761
                     /locus_tag="ACHOTW_004410"
     CDS             4667..>6761
                     /locus_tag="ACHOTW_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFP9846386.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSRSVININK"
BASE COUNT         1705 a         1706 c         1908 g         1442 t
ORIGIN      
        1 tcattatcct cagtattatc atagccgaaa acttctcccg gacacgggcg ttcacgataa
       61 tagtcatcgt catagttttc atcgtcagat aatgatatgt cgtattctga atatgattca
      121 ccacaattat acatgaaatc atctcctttg gggggggcaa taattagtga gtttattcga
      181 aaaaatgatg agcgccagac caatccaccc aacttttgat tataatcatg aagatacttg
      241 tctcagtaca aattgaatca aactcaatgc agcatgattc acctttatat tctctcaggg
      301 aaaccaaata ccactactca actattaaga acaaaattcg ggatgtatga ttatcagaag
      361 atacctgcat cctaaataat ttcagacaga tattatcgtt gtgaatcccc cgccaccttt
      421 ccggtgcgcg gggttttgtc ttttttcacc gggaatacat gtatgaatcc gtctgatgcc
      481 attgaggcaa ttgaaaaacc gctctcctcc ctgccttact cgctttcccg tcacatcctg
      541 gaacatctgc gcaaactcac cagtcacgaa cccgtgattg gcattatggg taaaagcggg
      601 gccggtaaat cctcactctg taatgcactg tttcaggggg aggtcacccc ggtcagtgat
      661 gttcacgccg gcacccggga agtgcggcgc ttccgtctga gtggccatgg tcacagcatg
      721 gttatcactg acctgcccgg ggtgggcgag agccgggaca gggatgcaga gtatgaagcc
      781 ctgtaccgtg acattctgcc tgaactggac ttggtactgt ggctgattaa agccgatgac
      841 cgtgccctgt ctgtggatga gtatttctgg cgacacatac tgcaccgggg acatcagcag
      901 gtgctgtttg tggtgacgca ggccgacaaa acagagccct gccatgaatg ggatatggca
      961 ggtattcagc cttctcccgc acaggaacag aacattcgcg aaaaaacaga tgcggtattc
     1021 cggctgttcc ggcccgtaca tccggttgtt gccgtatcgg cccgcaccgg ctgggaactg
     1081 gatacgctgg tcagtgcgct catgacagcg cttcccgacc atgccgccag tcccctgatg
     1141 acccgcctgc aggacgagct gtgcacggaa tctgtctggg ggcaggcccg tgaacagttt
     1201 accggtgcgg tggaccggat atttgacacg gtggagagcg tctgtgttgc ctctgttgca
     1261 cgcacagttc tgcgtgccgt ccgtgacacg gtggtctctg ttgcccgcgc ggtatggaac
     1321 tggattttct tctgaacagt ctgccagggg aaatgtctga ttacattgcg ttaaccaact
     1381 gaaaacatga tacaaacacc tcatgcccct cctgtttctc ttgcgtgact gctctactgt
     1441 taatagaata aaacgatcga taaaacaggt atcgatcgtt tatatcgatc gtttatagtg
     1501 gttaatggcc tgcgaaaagg cagctgaatc tcttcatcat gcagaacgga attgcacaca
     1561 acagactgat acttctctgt ctgatgaagg gggagagcgt ttacgcccgg gacgtatcgc
     1621 tgtgcccgat aactctgttt tgtacctgcc ggtatccact tttgtgggta ccggcttttt
     1681 tattcaccct ctgtaaggaa aagctgatga aacgacatct gaacaccagc tacaggctgg
     1741 tatggaatca cattacgggc accctggtgg tggcctccga actggcccgc tcacggggaa
     1801 aacgcgccgg tgtggcggtt gcgctgtctc ttgctgctgt cacatcagtc ccggcactgg
     1861 ctgctgacaa ggttgtacag gcgggagaaa ccgtgaacga tggaacactg acaaatcatg
     1921 acaaccagat tgtcttcggt acggccaacg gaatgaccat cagtaccggg ctggaactgg
     1981 ggccggacag tgaagaaaac accggtgggc aatggataca gaatggcggg atagccggaa
     2041 acaccactgt caccacaaat ggtcgtcagg tcgtgctgga ggggggaaca gccagtgata
     2101 cggttattcg tgacggcggg ggacagagcc tgaacggact ggcggtgaac accacactga
     2161 ataacagagg cgagcagtgg gtgcatgagg gcggggttgc caccggtaca attatcaacc
     2221 gcgacggtta ccagagcgtt aaaagtggcg ggctggcaac aggaaccatc atcaacaccg
     2281 gcgcagaagg cggccctgat tctgacaact cgtatacggg tcagaaggtc cagggaacag
     2341 cagaatccac caccatcaac aaaaatggac ggcagattat cttattttcc gggctagccc
     2401 gtgacactct catttacgca ggtggtgacc agtcggtaca cggaagggcc ctgaatacca
     2461 cactgaatgg cggttaccaa tatgtgcaca gggacggact tgcgctgaac acggtaatta
     2521 acgagggggg ctggcaggtt gttaaggcag gtggcgctgc cggtaacacc accataaatc
     2581 agaacggtga actgagggta catgccggcg gggaagccac tgcagtcacc cagaacacgg
     2641 gcggtgcact ggttaccagt actgctgcaa ctgtcatcgg cacaaaccgt ctggggaatt
     2701 tcacggtgga aaacggtaag gctgacggtg ttgttctgga atccggcggt cgtctggatg
     2761 tactggagag ccattcagca cagaataccc tagtggatga cggcggtacc ctggcagtgt
     2821 ctgccggcgg taaggcgaca agtgtcacca taacatccgg tggtgccctg attgcagaca
     2881 gtggtgccac tgttgagggg accaatgcca gcggtaagtt cagtattgat ggcacatccg
     2941 gtcaggccag cggcctgctg ctggaaaatg gcggcagctt tacggttaat gccgggggac
     3001 aggctggcaa caccactgtc ggacatcgtg gaacactgac gctggctgcc gggggaagtc
     3061 tgagtggcag aacacagctc agtaaaggcg ccagtatggt actgaatggt gatgtggtca
     3121 gtaccggcga tattgttaac gcaggggaga ttcgctttga taatcagacg acaccgaatg
     3181 ccgcgctgag ccgtgctgtt gcaaaaagta actccccggt aacgttccat aaactgacca
     3241 ccacgaacct caccggccag ggcggcacca tcaatatgcg tgttcgcctt gatggcagca
     3301 atgcctctga ccagctggtg attaatggtg gtcaggcaac cggcaaaacc tggcttgcgt
     3361 ttacaaatgt cggaaacagc aacctcgggg tggcaaccac cggacagggt atccgggttg
     3421 tggatgcaca gaatggcgcc accacagaag aaggtgcgtt tgccctgagt cgcccgcttc
     3481 aggccggcgc ctttaactac accctgaacc gtgacagcga tgaagactgg tacctgcgca
     3541 gtgaaaatgc ttatcgtgct gaagtccccc tgtatacatc catgttgaca caggcaatgg
     3601 actatgaccg gattctggca ggctcccgca gccatcagac cggtgtaaac ggtgaaaata
     3661 acagcgtccg tctcagcatt cagggcggtc atctcggtca cgataacaac ggcggtattg
     3721 cccgtggagc cacgccggaa agcagcggca gctatggctt cgtccgtctg gagggtgacc
     3781 tgctcagaac agaggttgcc ggtatgtctc tgacgacagg ggtgtatggt gctgcaggcc
     3841 attcttccgt tgatgttaag gatgatgacg gttcccgcgc cggcacggtc cgggatgatg
     3901 ccggcagtct gggcggatac ctgaatctgg tacacacatc ctccggcctg tgggctgaca
     3961 ttgtggccca gggaacccgt cacagcatga aagcgtcatc ggacaataac gacttccgcg
     4021 cccggggctg gggctggctg ggctcactgg aaaccggtct gcccttcagt atcactgaca
     4081 atctgatgct ggagccacaa ctgcagtaca cctggcaggg actctccctg gatgacggcc
     4141 aggataacgc cggttatgtg aagttcgggc atggcagtgc acaacatgtg cgtgccggtt
     4201 tccgtctggg cagccacaac gatatgacct ttggtgaagg cacctcatcc cgtgacaccc
     4261 tgcgcgacag tgcaaaacac agtgtgagtg aactgccggt gaactggtgg gtacagcctt
     4321 ctgttatccg caccttcagc tcccggggtg acatgagcat ggggacagcc gcagccggca
     4381 gtaacatgac gttctcaccg tcccggaatg gcacgtcact ggacctgcag gccggactgg
     4441 aagcccgtat ccgggaaaat atcaccctgg gcgttcaggc cggttatgcc cacagcgtca
     4501 gcggcagcag cgctgaaggc tataacggtc aggctacgct gaatatgact ttctgattat
     4561 acggtatccc ggaaatacga tttcaggaaa attcatgcag taatgggaaa ggacgctcat
     4621 tgctgttcac cgcagttctg aattattgaa tttataaagg tgaatgatgc acgaaaagaa
     4681 catcgccctg ctttgtgatg aagccgaccg gcttttgcaa ctgaatatca atctgctccg
     4741 gcaaatggtt gatgagccgg atgtgttatc tgacagtaag aacgaaaacg ggctgctttt
     4801 tgataaacgg aaagcgctga aaagaattga ggagctggag ggcgaacaaa tcaaaaccgc
     4861 ccgcagggag atggtgctgg ctgtggtcgg cacgatgaag gcgggcaaat caaccaccat
     4921 caacgccatt gtggggcagg aaattctgcc caaccgtaac cgccccatga cctcggtacc
     4981 gacattgatc cgccacgttc ccggaaaaac ggaaccggtc ctccatctgg aacatattca
     5041 gcccgtccgc aatttattaa tcacactgca ggaaaaactc gccaccccgg cagggcagca
     5101 agtcgcacag tccctgcagc aaaccgggga tacccgcgaa ctactggata ttctggcgga
     5161 tgatgtctgg cttaaaaatg aataccacgg ggaagacgaa atctttaccg gactggcatc
     5221 gttaaatgac ctggttcgtc ttgctgcggc aatggggact gaatttcctt ttgacgaata
     5281 cgcagaagtg cagaaactac cggtgatcga cgtggcgttc agccacctgg tgggaatgga
     5341 tgcatgtcag ggaacactca cactgctgga tacccccggc cctaatgaag ccggacaacc
     5401 acagatggaa atgatgatgc gggatcaact acagaaagcc tctgcggtgc tggcggtgat
     5461 ggattacacc cagatgaact cgaaagccga tgaggaggtt cgtaaagagc ttaatgccat
     5521 tgctgacgtc tcagccggtc gtctgtttgt gctggtcaat aaattcgatg aaaaagatcg
     5581 caatggtgat ggtgcggatg ccgtacgcca gaaggtgcca gcaatgctga acagcgatgt
     5641 gctgcccgcc tcccgcgttt atcccggctc atcacgtcag gcgtacctgg ctaaccgtgc
     5701 gcttcatgag ttacgaaaaa acggtgcgct tccggttgat gaagcctggg tcgatgattt
     5761 tatcagggaa gctttcggac ctatggtgga agaggacgac tggaaagaca gcactaaggt
     5821 aaataaaaag gcagaaaaac tctggaatat atctctgatc gatcaactga taacggaagt
     5881 catacagagc tcccattcca gagcggcggc actggcggtt gactctgccg ccgcaaaact
     5941 gatgcagaat gcagaaaatg tcagcgaata tctgtcatta cgtcatcagg ggctgcagca
     6001 aagcattcag tcactacagg cgcatattac cagcctgctg gcggatatcc aggagattga
     6061 ggaatgtcag aatcaggtga ccggtgatgt cagaatggcc atggaggaca tcaataccaa
     6121 aaccggggaa ttactgacga aggtctgtgc ctcactggag gaggagctga acgactattt
     6181 cagaagcggt aaacgcaaag aacagcaaat gctggaggaa gaaaattcag cgcagccccg
     6241 ggagagaaac gcattcgctt tctttcacga tattttcgga accggaaatc agcatgacag
     6301 gatgcgggac tttgatccgg acagccccga aataaaattc agtgatcgca gggcagccct
     6361 tgaactgatg acgcaaatcg aatcgaccgt gaccagcctg caccgtgagg ctgaagcaca
     6421 gttccggcct gagctggaga aaatcgtcag cgggattgaa acaggttttc gtggcacggc
     6481 cctgtacgcc acagaaaaca ttgccggtcg catcaatgcc cgcctggagg atgagggctt
     6541 taccgtaaaa atcagttttc cggcagtcag ccagttacag acccggctcg cggtaaaaat
     6601 aaatctgagt gcgcttatgg aggaaagaac ggagaccgtc acccgtcgcc gtcggcagag
     6661 tggcgtatgg ggaaccgttt gtcgatggtt tggcaccagt gacctgggct gggaaaacta
     6721 tgacgaggat gtgagtcgca gcgtgatcaa tatcaacaag g
//
LOCUS       DAZKIF010000198         6715 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZKIF010000198 DAZKIF010000000
VERSION     DAZKIF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.199"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..278
                     /locus_tag="ACHOTW_004411"
     CDS             <1..278
                     /locus_tag="ACHOTW_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFP9846387.1"
                     /translation="KKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDS
                     EGVDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
     gene            complement(506..664)
                     /locus_tag="ACHOTW_004412"
     CDS             complement(506..664)
                     /locus_tag="ACHOTW_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9846388.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAATGNWTRWIEI
                     LRVLEVVA"
     gene            complement(1324..1515)
                     /locus_tag="ACHOTW_004413"
     CDS             complement(1324..1515)
                     /locus_tag="ACHOTW_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFP9846389.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGEDL"
     gene            1580..1903
                     /locus_tag="ACHOTW_004414"
     CDS             1580..1903
                     /locus_tag="ACHOTW_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9846390.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQG
                     ARDDC"
     gene            2112..2543
                     /locus_tag="ACHOTW_004415"
                     /pseudo
     CDS             2112..2543
                     /locus_tag="ACHOTW_004415"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2581..3099
                     /locus_tag="ACHOTW_004416"
     CDS             2581..3099
                     /locus_tag="ACHOTW_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFP9846391.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSN
                     ETKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVT
                     HYQGKDWIQLTE"
     gene            3106..3339
                     /locus_tag="ACHOTW_004417"
     CDS             3106..3339
                     /locus_tag="ACHOTW_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFP9846392.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSERTERLSQLYSENWR"
     gene            complement(3714..3920)
                     /locus_tag="ACHOTW_004418"
     CDS             complement(3714..3920)
                     /locus_tag="ACHOTW_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846393.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQ
                     SGDGKLMATYRRNKKAIDKLDAEG"
     gene            complement(3913..4296)
                     /locus_tag="ACHOTW_004419"
     CDS             complement(3913..4296)
                     /locus_tag="ACHOTW_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846394.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKEL
                     ISKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINH
                     KISDTEDAVLSVGKIINLIEDSQHG"
     gene            complement(4687..5748)
                     /locus_tag="ACHOTW_004420"
     CDS             complement(4687..5748)
                     /locus_tag="ACHOTW_004420"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="HFP9846395.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMN
                     PIYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVI
                     KGNNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHE
                     IRKIFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKS
                     PILDQNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNI
                     NRIYTEMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPE
                     RRFDQSVYYTASHLFDEIK"
     gene            5836..>6715
                     /locus_tag="ACHOTW_004421"
     CDS             5836..>6715
                     /locus_tag="ACHOTW_004421"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP9846396.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSH
                     PIKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKR
                     IKPKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGK
                     LDVRDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTV
                     RGVNRPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGN
                     AVPVELAKYVAVTIMEY"
BASE COUNT         1768 a         1310 c         1751 g         1886 t
ORIGIN      
        1 aaaaaaaaca agtaaaaggt atttatgata aagaaggctt tagggcgtgg ttgctgaatg
       61 aaaagaaact aacaaaaaga acctctagcg atattatatc tagatgttgc agaggtgttt
      121 cattttttga ttcagagggg gtggattttt ataactgtga aatagatgaa attattatga
      181 aactggaaag gcttgaatcg tttgtcaggc ttggggttag cctgaagtct cagttaagaa
      241 gggcttttaa attatattac gaatattgtc gaaggtaata aatacgaatt tatgtctctc
      301 aggggagggg gaggggataa aaagatcaaa ggatcctctt gtgatccttt ttttctgcgc
      361 gtaatctgct gcctgtaaac aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa
      421 tctagagcta ccaactcttt cttccgaagg taactggctt ggtggagcgc agataccaaa
      481 tactgtcctt ctagtgtagc cgcagttagg ccaccacttc aagaactctt aatatctcaa
      541 tccaccttgt ccagttacca gtggctgctg ccagtggcgt taagtcgtgt ctttccgggt
      601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccaa cagcgtgagc
      721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tctgtgatgc tcgtcagggg
      901 ggcggagcct atggaaaaac gccagcggcg cggccttttg ttgtcctttg cctttttgct
      961 cacatgttct ttccggcctt atcccctgat tctgtggata accgtattac cgcctttgag
     1021 tgagccgact ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     1081 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1141 atatggtgct cttttcttat aatccgcact gatgccacat agttaagcca gtatacactc
     1201 cgctatcgct acgtgactgg ttcagggctt cgccccgaaa ccccctgacg cgccctgacg
     1261 ggcttgtctg ctcccggcat ccgctcacag gcaagctgtg accgtctccg ggagctgcat
     1321 gtgtcagagg tcttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     1381 ggcgtggtcg tgaagagatt cacaaatatc ggcctgttca tctgcgtcca gttcgttgag
     1441 tttctccagc agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     1501 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     1561 accgatgaaa cgcgagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     1621 actggaacgc tgtgagggta aacaactggc ggtatggatg cggcgggtct gcctgggtga
     1681 gccggttgcc cgttccggga agctgccgac actggcaccg ccgttactgc gtcagctggc
     1741 cgccatcggg aataacctga atcagacagc gaggaaggta aacagcgggc agtggtcttc
     1801 cggtgaccgg gttcaggtgg tggccgcact gatggccatc gagggagagt tgcgcagctt
     1861 acggcaggtc gtcagggagc agggagcgcg ggatgattgt taaatttcat gccaggggaa
     1921 aaggcggtgg cagtggtccg gttgattacc tgctgggaag ggagcgtaac cgggaagggg
     1981 caacggtgct tcagggtaat ccggaagaag tcagggaact catcgatgcc acgccatttg
     2041 cgaagaaata cacgtccggt gttctgtcgt tcgcggagaa ggaactgccg ccgggaggac
     2101 gtgaaaaagt gatggagagc tttgagcgcg tactgatgcc cggtctcgaa aaaaatcagt
     2161 acagcattct gtgggtggag caccaggaca aggggcgtct tgagctgaat tttgtcattc
     2221 cgaacatgga gctacagacc ggaaaacgcc tccagccgta ctacgaccga gcagacaggc
     2281 cgagaattga tgcctggcag acactggtga atcaccatta cgggctgcat gaccccaacg
     2341 ccccggagaa ccgccggata ctgactctct ctgataacct gccggaaacg aaacaggcgc
     2401 ttgctgagag cgtcacacgc gggatagacg ctctttatca tgtaggggag ataaaaggcc
     2461 gtcaggatgt gattcaggcg ctcacagagg cggggcttga agtggtcagg gtgacgcgaa
     2521 gcagcatcag cattgcagac ccgaacggcg ggaagaatat caggctgaaa ggagcgtttt
     2581 atgagcaatc ttttacagac gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa
     2641 tctacagaga aaatgctgaa cgacgagttc aggaagctcg gcgagtctgt cagcgaggct
     2701 gtgacctcaa acgagacgaa aatcagagac gctatagccc tgttcacagc ctcgacagag
     2761 aaatcgctgg aaaaacacag ggaaggggtg aaagaggcga tgatgcagca caggaaggac
     2821 gtgttaaagc tggcagggaa tacgggcatg atgttactgg ggatagtctt tctcctgttt
     2881 accgcgagtg gcgggacgct ctggtatctt ggcgggagga tacaggcgaa cctggaagaa
     2941 atcaggaagc aggaagagac attgcagaaa ctgaacgcga agacatgggg cgtggagttt
     3001 gtgcaggacg ggagaaggaa atttctggtc attccgcaag ggatgtcagc agaggtgacg
     3061 cactatcagg ggaaggactg gatacaactg acggagtgat acggaatgac agagctggaa
     3121 acacatttgc tgaacgcctt agagcagctg caacaggact atatgcagcg gctgaacgaa
     3181 tgggagagcg ccttcgtgga attgcagaag atgttttcgc ttacgcaacg ggacaacgcg
     3241 atgctgaacg agcgggtcat gcagttgagt cagcaggtgc agcacttgag cgagcggaca
     3301 gaacgcttga gccagttata cagcgagaac tggagataag agaggaacgg ctgatacagg
     3361 agcgcgaaca tgcgttatcc ctggaacggg agcgtcagcc ggaaatacag gaacgcatac
     3421 tggatggtcc ttcgttgtcg ctgtgatggc gaaactataa aaaatggcag gttcagtgga
     3481 ttttgacgga ctaatgtagt ctgcaccatc tggttgcata ggtattcatg cggttaaaat
     3541 ttatcaggcg cgatcgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
     3601 gccgtgatcg caccgaacgc gttttagcgg tgtgcgtaac ggatgttatg gtaaatacaa
     3661 tatgcagcca gtcttagaga cgaaaagtcc gcccagaaga ctgacttttc agatcaacct
     3721 tcagcatcta acttgtctat cgctttttta ttgcgccgat aggtagccat tagtttacca
     3781 tcgcctgatt gtaagtcacg acctgcttgc tcagcatagg tatcgtttac agtcttagcg
     3841 ccttttttat aggccttttt aacatcatca aatccggctt ggatgacttc tttcggcttt
     3901 tcttgaggta atttagccat gctgtgaatc ctctataagg ttgattattt ttccaacaga
     3961 taagacggca tcttctgtgt cgctgatttt atgatttatc caaccgtcct ctatctttaa
     4021 tttgttcact actcctaatg taaaaacaat ctttttttca ggaaagaaaa ttgcaaaggt
     4081 tctacttttc tctttccaat cagagggcgg cgtcgaacgg aacgctacat tcctcgattt
     4141 aagtatcgct ttgattttac taattaattc ttttttttgt ttattgctga gatttttgac
     4201 gaaccctttg ctgagattgt ctgtgtaatc atcaaaagaa atatcaactg gtttgttttt
     4261 ttgtttttta tcagatagat gaacaatgtt tgacactgga tatcctgctt tttgatgaat
     4321 gaatactcag aagagtagcg gaactgtctt gttagtcaca gaggtagagt atcaattcta
     4381 cgatagatgg gttcgaagat ccggctgaga ccgttcagta cgcggtcgag atgttatgtt
     4441 caatgtaatg accgtcgaaa ttgcctttac gggcatgaag caaccaggag caatcattgg
     4501 caaaggtaac tagagttcaa tgcatggtaa tcatttttga gcttgtgatg catagagttg
     4561 gttgctgttc cattgcgtct caaactccgt caagatgctc gccgtaaaag cgagcatgtc
     4621 tagtatgcct gttccgctac tgatttttga tgggcgaatt cagggctggc gagccaccaa
     4681 gaaaaattac tttatttcat caaacaggtg agatgcagta taataaacgc tttgatcaaa
     4741 gcgtctctct gggcttaatt tattttgccc gccatttaaa attatttctt ttaatactga
     4801 tggtgggagg atgggttttg ttcttcttgg gtgatccata taagcttttg ttatttttcc
     4861 gtttagcgag aactctttat cactttgata tccaaagttc atctcagtat aaattctatt
     4921 gatgtttgtc ttgtttttgc tcgcaatttc atacaccatt ctggcgagtc tggctgatga
     4981 tctcattagt ctgctggcag atgaattgtt atatgaggat ctatcaataa aggttcgaca
     5041 caaagcaaaa tcactccaga tgaatatgtc aaaggcattt tgatctaata ttgggctctt
     5101 gccttttgtt ttccataatg gttgcattaa aaaaggtttc tggtaatcaa gaaaattttc
     5161 ttggaaagtg tttaggcaat ttaatatatt ttctttcttt gaaagaactt cagtttcatt
     5221 cccccagtgc tgtatgcttt cgcatgtgga ttcaaatatt tttcttattt catggaattt
     5281 atcgctgcaa gagtgcatta tgccaagggc acaataagaa gtgcttgcag gtcttattac
     5341 aatctctgaa ctccattcgc tctcgttttc tttatatgtc gtgctatctg gaagtacggt
     5401 taattttatt tctagtgctt ttaaaaaact attgtttcct tttataacta gatctattcc
     5461 ttttatgtca tcataagcat actgttggta tggttcatat tttttctcaa attgaaattc
     5521 aagatcacta ttttttaatg ttgtgttaaa aacgaagtca aaagatactt ctttggcatg
     5581 cacttttaaa tccttatcta aataaagata aatcggattc attttgttgt ctctcatata
     5641 acatgctaga gaggtgggaa aagaactgtt aaattggttc tttccccaca gatcgctctc
     5701 tttcctgttg gtgtttttta aaccataaag agattctatt ttgctcactt tttgctcctg
     5761 acgtcaattt attgacgtct atggtgtgtt tctatatcat gatgtggagt gatatgataa
     5821 tgagtttttt atgttatgag aataattgat ctttttagtg ggtgtggggg gttgtcccta
     5881 ggctttttga agggggggtt tgatgttgta ggtgcttatg atttctggga tcctgctatt
     5941 gaatgttaca gagataattt tagccatccg ataaaaaaac tagatttgag caatgttgat
     6001 gatgtggtga gggagcttaa agatatagac ttcgatatga ttattggagg gcctccttgc
     6061 caagacttca gtcatgcagg tttaagaatt gaaggtgcta gagctaactt aactcgctca
     6121 ttttcagaaa ttataaaaag aataaagcca aagtggtttg tgatggaaaa tgtagatcgg
     6181 gcattaagaa gtggtgccta tttagaagct cgaggaatct ttaaagaatc agggtatggg
     6241 ttaacagaaa ttgttttgga tgccagtaag tgtggtgttc cgcaaaaaag aaaaagatta
     6301 tttgtaattg gaaagcttga cgtgagggat ggttttatcc ttaacgaagt gatgtgcggg
     6361 atatcgaaag atagtatgac agttagaaat tatcttggtg attcattggg gattgagtat
     6421 tattacagac atccgagaaa ttataacaga agggctattt tctctattga tgaacctgcg
     6481 ccaacagtaa gaggggttaa tagaccgata cctgatggat atttagggca tgcaggcgat
     6541 cctgtcagta tttctgagaa tgtaaggcca ttaacaacct ttgaaagagc aagattgcaa
     6601 acatttcctg aagactttaa atttaaaggt gcaaaaacaa atttggagca aatgataggt
     6661 aatgctgttc cggttgagtt agcaaaatat gttgcggtga caatcatgga gtatg
//
LOCUS       DAZKIF010000199         6676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZKIF010000199 DAZKIF010000000
VERSION     DAZKIF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.200"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(566..1372)
                     /locus_tag="ACHOTW_004422"
     CDS             complement(566..1372)
                     /locus_tag="ACHOTW_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9846397.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPNLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSKEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(1373..1678)
                     /gene="ccdB"
                     /locus_tag="ACHOTW_004423"
     CDS             complement(1373..1678)
                     /gene="ccdB"
                     /locus_tag="ACHOTW_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFP9846398.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1680..1898)
                     /gene="ccdA"
                     /locus_tag="ACHOTW_004424"
     CDS             complement(1680..1898)
                     /gene="ccdA"
                     /locus_tag="ACHOTW_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFP9846399.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2441..3040
                     /locus_tag="ACHOTW_004425"
     CDS             2441..3040
                     /locus_tag="ACHOTW_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9846400.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            3037..3657
                     /locus_tag="ACHOTW_004426"
     CDS             3037..3657
                     /locus_tag="ACHOTW_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846401.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            3654..3854
                     /locus_tag="ACHOTW_004427"
     CDS             3654..3854
                     /locus_tag="ACHOTW_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846402.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            4262..5239
                     /locus_tag="ACHOTW_004428"
     CDS             4262..5239
                     /locus_tag="ACHOTW_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9846403.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(5524..6264)
                     /locus_tag="ACHOTW_004429"
     CDS             complement(5524..6264)
                     /locus_tag="ACHOTW_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9846404.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(6385..6615)
                     /locus_tag="ACHOTW_004430"
     CDS             complement(6385..6615)
                     /locus_tag="ACHOTW_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFP9846405.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
BASE COUNT         1643 a         1769 c         1718 g         1546 t
ORIGIN      
        1 gcatggataa aggcctacaa ggcgctctaa aaaagaagat ctaaaaacta taaaaaaata
       61 attataaaaa tatccccgtg gataagtgga taaccccaag ggaagttttt tcaggcatcg
      121 tgtgtaagca gaatatataa gtgctgttcc ctggtgcttc ctcgctcact cgagggcttc
      181 gccctgtcgc tcgactgcgg cgagcactac tggctgtaaa aggacagacc aaacatcatg
      241 gttctgtgtt cattaggttg ttctgtccat tgctgacata atccgctcca cttcaacgta
      301 acaccgcacg aagatttcta ttgttcctga aggcatattc aaatcgtttt cgttaccgct
      361 tgcaggcatc atgacagaac actacttcct ataaacgcta cacaggctcc tgagattaat
      421 aatgcggatc tctacgataa tgggagattt tcccgactgt ctcgttcgct tctcagtgga
      481 taacagccag cttctctgtt taacagacaa aaacagcata tccactcagt tccacatttc
      541 catataaagg ccaaagcatt tattctcagg ataattgttt cagcatcgca accgcatcag
      601 actccggcat cgcaaactgc acccggtgcc gggcagccac atccagcgca aaaaccttcg
      661 tgtagacttc ctttgaactg atggacttat gtcccatcag gctttgcaga actttcagcg
      721 gtataccagc atacagcatg tgcatcgcat aggaatggcg gaacgtatgt ggtgtgaccg
      781 gaacagagaa cgtcacaccg tcagcagcag cggcggcaac cgcctcccca atccaggtcc
      841 tgaccgttct gtccgtcact tcccagatcc gcgctttctc tgtccttcct gtgcgacggt
      901 tacgccgctc catgggtatt ttcagtgttg ccaccatcgt ctgcagctgg ctgacgtacc
      961 aggagtcaga gagcggaacc agccggtgag tctgctgacc ggcgggcatt ctccccgccg
     1021 tcctggcagc tttttcggtc cgttgtttca gggtcgcaag ctgcacaaac ggatacggag
     1081 gcgcaagcga aaaatccccc cgcgtcagcg ccagtgcttc attaatgcgt gctccggtgt
     1141 tccacagtgt ggccagcagc atcttgcggt gcagattcgg gacgtaatgg agcagggcac
     1201 tcacttccgg agccagcaga tattttggca gttcatcatg gaccatcgac atctggcgaa
     1261 gtgccagagc tgccggataa tcaatggcaa caggcagcga tgcaggctgt ccggcagaat
     1321 acactgccgg taccatgact gcagactggc tgtgtataac ggagcctgac atttatattc
     1381 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1441 cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc
     1501 tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac
     1561 gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta
     1621 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt
     1681 caccagtccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca
     1741 gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc
     1801 tgcatggttg tgcttaccag accggagata ttgacatcat atgccttgag caactgatag
     1861 ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag cacacctctt tttgacatac
     1921 ttcgggtata catatcagta tatattctta tgccgcaaaa atcagcgcgc aaatacgcat
     1981 actattatct ggcttttagt aagccttatg tattttacct ttcgttatgt taaccaataa
     2041 aaattaaaat ctgccttata aaaacaaagc gtaattaccg cattcccgtt tcgtatggat
     2101 ccgactttat ttgaataaac gactttccgg aagtgtacca cctgagaaaa tcatcatatc
     2161 gctgttttcc gcccctgcgc ggcgcatcac agtccagaga tacaacacaa catatagtgc
     2221 catgggcaac tgaatgaggc actgcatgct gtgctgacgg gatgtaacgc tgtgcggagg
     2281 gagaggaaga tccgtaacca ccagaaaccg gatctgacca ggaaaagaga aagagtgtat
     2341 gtaagttctg tactgtatgt atgaattgta ttgtatgtat tgtgtatttt tcataccggg
     2401 catacgatgg cgccattccc tgagaaaccg gagccagacc atgacatccc tctccccgga
     2461 caccgtccgc cgcattgaag acgccgccgc cgccctgatc gccgccggca ccccgaaccc
     2521 gaccaacgag caggtccgtc agcacctcgg cggcggctcc ctgtcccaca tctccccggt
     2581 gatgcgcgcg ttccgcgccc gccagcgcga gcaggccacg cccctgccgc cggagctggc
     2641 gcagctgctg accggccagc tggggctgct gtggcaggcg gccgtgaaac aggctgaagc
     2701 cggggccctg gcggcgcgcg agcaggccga cgacgacatt gcccgggccg acaaggagcg
     2761 ggatgaggcg ctggcgaacg tggccgcgct ggaaagcgag ctggcggtgc tgcgcgaggt
     2821 ggtggccgaa cgtgaccgcc tgctgcagga ggtacgcgag ctacgcgcgg aggcgctgcc
     2881 gctgcgcgag caggtggccc gcctgaccgc caccggcgaa cacctggcgg cgcagctgca
     2941 ggacacgaag gcggagctga aggaagcccg ggaggacggc cgccagcttc agaccgaact
     3001 gctggctctg gcccgccagg acgggaaggt gaagaaatga gacaggaaag agtattacgt
     3061 ataaaggcat ttcgacgggc actggagctt ggtggcggtg aacgggctga cgggtgtcgt
     3121 cattttcccc gctggaaaac ggaactgagg aatttcccgc acagcagctg cgatttagcc
     3181 agcaatactc tggcgtacta cctcaaagaa cgagagagat gccatccgtg catcattttt
     3241 atggaaggga acgctggctt tcatgaagcg gaaaactcaa cagttcacgg gcatgtcatc
     3301 gtattgctgg acggagagta catcgatttg acgctggatc aattcgacga atatccggac
     3361 tacattcctt acgaacctgt cgaatctgac gggcagattg gtaagctgtt gcgtaatatc
     3421 atgaagcatg aggacccggt aaaaacccgc cagattgatc tcgatggtgg cgaagagctg
     3481 tatgcctggt tacgcgatac cgccgacgaa gtgctggcag cagatccgga atggcaggcc
     3541 cgggaacggt cgatagaaga agcaagggaa gccgctatta cggtgttccc gtttctgtct
     3601 gacgtgcaaa aaactgagtg cgaacagggc ccggacagtc aggaggctgc cagatgaaga
     3661 tttatcatta cactgacctg aacggcctga agggcatcat cgaatcaggc tcactgtggg
     3721 cgacgcactt ttcctttctc aatgacagta atgagctgac tcacggcatg aactgccttg
     3781 agaatgccct gcagtacctg agggacgact ttaacccgaa aacccttaag ttcatcgaac
     3841 aggcaccctt ataagcggtt gtgtatgctg tttccctgga atgatttatc cacatatccg
     3901 gaggctcgat ccagtaaaca gatccatgaa tgatcaacaa aggatccatt aaagatcccc
     3961 ataccgctgc aaaccttgtc actcatgggc cgggaccacg atcacataag cagtggcatg
     4021 ttactgataa actgtaacat gctaatgata agctgtattc agtaatccat atactgaagt
     4081 aagttaatga cataaactat ggtcagtacg ccagactcag ctgttaaata caggctgcag
     4141 gtttttcttc agtcagttag cggggctctg acacacgatt tgctgtttat tcttttactg
     4201 tccacaggca ggaggctttc tggaaaacga aaattcagac atcaaaaaac tgttcggcga
     4261 ggtggataag tcgtccggtg agctggtgac actgacacca aacaataaca acaccgtaca
     4321 acctgtggcg ctgatgcgtc tgggcgtttt tgtaccgacc cttaaatcac tgaagaacag
     4381 taaaaaaaat acactgtcac gtactgatgc cacggaagag ctgacacgtc tttccctggc
     4441 ccgtgctgag ggattcgata aggttgagat caccggcccc cgcctggata tggataacga
     4501 tttcaagacc tgggtgggga tcattcattc ctttgcccgc cataacgtaa ttggtgacaa
     4561 agttgaactg ccttttgtcg agtttgcaaa actgtgtggt ataccttcaa gccagtcatc
     4621 acgcaggctg cgtgagcgca tcagcccttc cctgaaacgc attgccggta ccgtgatctc
     4681 cttttcccgc accgatgaga agcacacccg ggaatacatc acccatctgg tacagtcagc
     4741 ctactacgat actgaacggg atattgttca gttacaggct gatccccgcc tttttgaact
     4801 gtaccagttt gacagaaagg tccttctcca gcttaaggcg attaatgccc tgaagcgacg
     4861 ggagtccgcc caggcactct acacctttat agagagcctg ccccgggatc cggcaccggt
     4921 atcgctggcg cggctgcgtg cacgcctcaa tctgaagtct cctgtatttt cccagaacca
     4981 gacggtcaga cgggcaatgg agcagctgcg cgagattgga tatcttgatt acacggagat
     5041 ccagcggggg cggacaaaac tcttctgcat tcactaccgg cgtccccggt taaaagcacc
     5101 gaatgatgag agtaaggaaa atccgttgcc accttcacct gcggaaaaag tcagtccgga
     5161 gatggcggag aagcttgccc tgcttgagaa actgggcatc acgctggatg acctggaaaa
     5221 actcttcaaa tcccgctgaa cataaactgt agtcagtgaa gagtgttcct ttactgacta
     5281 cagcttatat tatcaggtgc agtgagtggt ctgctcactg cagtttatat tcagtttcct
     5341 gcagtgctgc ctgtagctga gctgtcatct gccggtccct tacgtgagtc accccgtaac
     5401 ctgatgctga ggcattgctc ccttcataaa acatgactta ctcactacag cttatataca
     5461 tgctccagct tatgttatgt ctgttctgct gaccacagct tatataagga agcgcgtatg
     5521 ccgtcacctc aggggaggca gtgtacgcag gatctccgca gcatcccgcc cgtcacctgt
     5581 gaaaggcact gccagcgtgg cagccatatc cagcgcaaac actctggtat aaacctccat
     5641 cgaacgtgga tccctgtgac ctgccagggc ctggatgact ttccggggct ggcggtgata
     5701 gagcatatgc atgatatagc tgtgccggaa ggtgtgtggt gtgaccggaa tcgaaaagtg
     5761 tactccgtca gcttcggccc gtctgacagc ctgcttcagc cagttgcgca tggtttcgtc
     5821 ggtcacggcc cataatggtt cacgacgacg gggccgggtg gtgatcatcc agctttccat
     5881 ctgcctgaca tagcttatat ccgtcagcgg aaccaggcgg acttcatctt ttggcgggcg
     5941 tccgcgtcgc gcacgcactt tttcggacag gatccgcaca aacggccttg ctccatccag
     6001 gtcaaatgat tccggcgtca gcatccgggc ttcgccaata cgcattccgg tattccagag
     6061 ggtggcgaac agcatatggt gacgctgatc cggcatatag aaaagaaggg cactcacttc
     6121 cggggccagc aggtaggccg gtgtggcccc tgtggaagtg gccattcttc tcagggagag
     6181 cgctgttgca aaatccaccc ccggcgcaat gggtaacagg aagacgcgtt ctggtgaatt
     6241 ctgcagggga atgacattgt tcatgagtta tttttcacgt agcacacgca ctgtttatgg
     6301 atacaggtat tctgacctga acgcccggat ccttccacga caataaatcg cagcgtgcgg
     6361 atccttttgt ttgcttatca cgcattattg cgggtaaaac caaatacgta aaggtacgtt
     6421 ttgcagcagg gatcccgtat taagccagtc gtggcgcggg ctgagggctg gtttgccgtt
     6481 tttgggagaa ttggggtaat gccaggacgc taatgatcta tcaatggatg gatgtaagtg
     6541 acaattgaaa actgaaaaat gcgctatgta gattctgaca gatttatgca cctgaaatac
     6601 gttcaaaatc cacatagcgt aactctgatt ttccttacca gaaaaaagag ggggaataac
     6661 cagtatctat cccccc
//
LOCUS       DAZKIF010000200         6549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZKIF010000200 DAZKIF010000000
VERSION     DAZKIF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.201"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2937
                     /gene="ehxA"
                     /locus_tag="ACHOTW_004431"
     CDS             <1..2937
                     /gene="ehxA"
                     /locus_tag="ACHOTW_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFP9846406.1"
                     /translation="FNTASSSVRSAGKKLILLIPDNYEAQGVGINELVKAADELGIEI
                     HRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDKLLQKYQKVGSKIGGTAENVG
                     NNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGEDISQNDIAKSSIELINQLVDT
                     VSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNLPDLGPLGDGLDVVSGILSAV
                     SASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQYILAQRMAQGLSTTAASAGLI
                     TSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNYEGDALLAAFHKETGAIDAAL
                     TTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTISGILEASKQAMFEHVAEKFA
                     ARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFSRQHAVERAVAITQQHWDEKI
                     GELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQQVFDPQKGTIDLSTGNVSSV
                     LTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWSVKGIKNHKGVYDYSKLIQFV
                     EKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGYDTVSYNKTDVGKLTIDATGAS
                     KPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQYRDFEFRTGGIPYDVIDNLHS
                     VEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGNDRLYGDDGDDYISGGQGDDQL
                     FGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSGGTGDDKLYGGGGIDLLDGGE
                     GNDYLNGGFGNDIYVYGQNYGHHTIADEGGKGDRLHLSDISFDDIAFKRVGNDLIMNK
                     AINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVITDKDGREIKVDKIPHNNNER
                     SGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSGDERLASLYNLSLHQNNTHST
                     TLTTTV"
     gene            2987..5107
                     /gene="ehxB"
                     /locus_tag="ACHOTW_004432"
                     /gene_synonym="hlyB"
     CDS             2987..5107
                     /gene="ehxB"
                     /locus_tag="ACHOTW_004432"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFP9846407.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRITKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFIRELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            5111..>6549
                     /locus_tag="ACHOTW_004433"
     CDS             5111..>6549
                     /locus_tag="ACHOTW_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFP9846408.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
BASE COUNT         2103 a         1023 c         1483 g         1940 t
ORIGIN      
        1 tttaatacag catcatcaag cgtacgttcc gctggaaaaa aactcatatt attaatacct
       61 gataattatg aagctcaggg cgtgggtatt aatgagttgg tcaaagctgc tgatgagctt
      121 ggaatagaaa tacaccgtac tgaacgagat gatacagcga ttgcaaacca gttttttggt
      181 gcagcagaaa aagttgtagg attaactgaa cgtggtgttg caatattcgc accacaactt
      241 gacaaacttc tgcagaagta tcagaaagtt gggagtaaaa taggaggaac cgctgaaaat
      301 gtaggtaata atctgggaaa agccggaaca gttctctcag cactacagaa ttttacgggg
      361 attgctttat caggcatggc tcttgatgaa ttgctgagaa aacaacggga aggagaggat
      421 ataagtcaga atgatattgc caaaagtagt attgaactta ttaatcagct tgtagataca
      481 gtatcaagta taaacagtac cgttgattca ttttctgagc agcttaacca gcttggctca
      541 tttttatcca gtaaacctcg attaagttct gttggtggga aattacaaaa tttaccagac
      601 ctgggccccc tgggggatgg gctggatgtt gtctccggaa ttctttctgc tgtatcagca
      661 agctttattc tgggaaacag tgacgcacat acaggaacaa aagctgcagc gggtatcgaa
      721 ctgacaactc aggttcttgg aaatgttggt aaagctgttt cgcaatatat tctggctcag
      781 agaatggcac aggggttatc gacaacagct gcaagtgcgg gtctgatcac atcggctgtt
      841 atgctggcta tcagtcctct ttctttcctg gctgctgcag ataaatttga gcgagctaag
      901 cagcttgaat catattctga acgatttaaa aaattgaatt atgaagggga tgctttactc
      961 gcagcctttc ataaagaaac cggagctata gatgcagccc tgacaacaat aaatactgtc
     1021 ctgagttctg tatctgcggg agttagtgca gcctccagtg catccctcat aggggccccg
     1081 ataagcatgc tggtgagtgc attaaccggt acgatatctg gcattctgga agcatcaaaa
     1141 caggctatgt ttgagcacgt tgcagagaaa ttcgctgctc ggatcaatga atgggaaaag
     1201 gagcatggca aaaattattt tgagaatgga tatgacgcaa gacatgctgc gtttttagaa
     1261 gactctctgt ctttgcttgc tgatttttct cgtcagcatg cagtagaaag agcagtcgca
     1321 ataacccagc aacattggga tgagaagatc ggtgaacttg caggcataac ccgtaatgct
     1381 gatcgcagtc agagtggtaa ggcatatatt aattatctgg aaaatggagg gcttttagag
     1441 gctcaaccga aggagtttac acaacaagtg tttgatcctc aaaaagggac catagacctt
     1501 tcaacaggta atgtatcaag tgttttgaca tttataacac caacatttac cccaggagaa
     1561 gaagttagag aaagaaaaca gagtggtaaa tatgaatata tgacatctct tattgtaaat
     1621 ggtaaggata catggtctgt aaaaggcata aaaaatcata aaggtgtata tgattattca
     1681 aaattgattc agtttgttga aaagaataac aaacactatc aggcgagaat aatttctgag
     1741 ctcggagata aagacgatgt ggtttattct ggagcaggct catcagaagt atttgctggt
     1801 gaaggttatg ataccgtatc ttataataag acggatgttg gtaaactaac aattgatgca
     1861 acaggagcat caaaacctgg tgagtatata gtttcaaaaa atatgtatgg tgacgtgaag
     1921 gtattgcagg aagtcgttaa ggaacaggag gtgtcagtag ggaagcgaac agagaaaata
     1981 caatatcgtg attttgaatt cagaaccggt ggaattcctt atgatgtaat agataatctt
     2041 cattctgttg aagagctcat tggcggaaaa catgatgatg aattcaaagg cggtaagttt
     2101 aatgatatat tccatggcgc agatgggaac gattatatcg aaggtaatta tggtaatgat
     2161 cgactatacg gcgatgatgg ggatgattat atatccggag gacagggaga cgaccagtta
     2221 tttggtggta gtggaaacga taaattgagt ggaggggatg gtaataatta tctgacagga
     2281 ggaagcggta atgatgagct tcaggcacac ggagcttata atattctgtc aggtggtact
     2341 ggtgatgata aactttatgg tggtggtggt attgatcttc tggatggagg ggaaggtaat
     2401 gactatctga atggtggttt tggtaatgat atttatgttt atgggcaaaa ctatggtcat
     2461 catacaattg cagatgaagg aggtaaagga gatcgtttgc acttatctga tattagcttt
     2521 gatgatatcg catttaagag agttggaaat gatcttatca tgaataaagc cattaatggt
     2581 gtactttcat ttaatgagtc aaatgatgtc aatgggataa catttaaaaa ctggtttgcg
     2641 aaagatgcct caggagcaga taatcatctt gttgaggtta taacagataa agatggtcga
     2701 gagataaaag ttgataagat acctcataat aataatgaac ggtcaggtta tataaaagcc
     2761 agtaatatag catctgaaaa aaacatggtt aatatcacca gtgttgccaa tgatattaat
     2821 aagattattt cttcagtttc agggttcgat tcaggtgatg aacgattagc atctttatat
     2881 aatttatcct tacatcaaaa caacacacac tcaacaactt taacgacaac tgtctgagac
     2941 taaaagagga ggtgatggcc attccaggag gaatggtcat cgttatatga tgagtaaatg
     3001 tagttctcat aatagtctgt atgcactgat attgcttgca caatatcata atataactgt
     3061 caatgctgaa actataaggc atcagtataa tacccacaca caagattttg gggtgactga
     3121 atggttactg gcagcgaaat ctattggctt aaaagcaaaa tatgtagaaa aacatttttc
     3181 cagattgtca ataatttctt tacctgcgtt gatatggcgg gatgacggta agcattatat
     3241 attgtctcgt attactaaag attcatcacg ctatcttgtt tatgatccag aacaacatca
     3301 gtcactaact tttagtcggg atgagtttga aaaactgtat cagggaaaag tcattctggt
     3361 tacgtcaaga gcaacagtag tcggagagtt agctaaattt gatttttctt ggtttatccc
     3421 ctctgttgtg aaatacagga ggattttact tgaggtgtta actgtttctg cttttattca
     3481 gtttcttgcg ttaataacac ctcttttttt tcaggttgta atggataagg ttttagttca
     3541 ccgggggttt tcaacgttaa atattatcac aatagcattt attatagtga tactttttga
     3601 agtgatatta accggagcca gaacttatat tttctctcat actacaagtc gtattgacgt
     3661 cgaactgggt gctaagttat tcagacattt gcttgcattg cctgtttcat attttgaaaa
     3721 tcgcagggtc ggagagaccg ttgccagagt aagggaactg gagcaaattc gtaatttttt
     3781 aaccggacaa gcgttgacat cagttcttga tctatttttt tctgtaatat ttttttgtgt
     3841 catgtggtat tacagccctc aattaacact ggttatatta ttgtcactac cttgttatgt
     3901 tatatggtca ttgtttatat cacccttatt acgtcgacgt cttgatgata agtttctcag
     3961 gaatgcagaa aatcaagctt ttcttgtcga aacggtaaca gcaataaata caatcaaatc
     4021 catggcagta tcaccacaga tgattgctac atgggataaa caactggccg ggtatgttgc
     4081 ttccagtttc agggtaaatc tggttgcaat gacagggcag caggggatac agctgataca
     4141 gaaaagtgta atggtaatta gtctatggat gggagcacat cttgtcatat cgggagagat
     4201 aagcatcggg cagttaatcg cgtttaatat gcttgcaggt caggtcattg ctcctgttat
     4261 cagactggct catctttggc aggattttca gcaggttggt atttctgttg agcgccttgg
     4321 tgatgtatta aatacaccgg tagagaaaaa gtcaggcaga aatatactgc cggaaattca
     4381 gggggatatc gaatttaaaa atgtcagatt tcggtattct tctgacggta atgttatttt
     4441 gaataatatt aatttataca tatcaaaggg ggatgttatc ggtatagttg gccgttctgg
     4501 ttcaggaaaa agtacattaa caaaactgct tcagcgcttt tatataccag agaccggaca
     4561 gattttaatt gatgggcatg atttatcact tgcagatcca gaatggttac gacgccagat
     4621 tggtgttgta ttgcaggaaa atatactatt aaatcgtagt attatcgata atattacatt
     4681 agcttctccg gctgtatcta tggaacaggc tattgaggca gccagacttg caggtgccca
     4741 tgattttatt agagaactaa aagaagggta caatactatt gttggagaac agggcgttgg
     4801 tctttcagga gggcagcgcc aacggatcgc aatagcccgg gctcttgtta caaatcctcg
     4861 aattcttatt tttgatgaag caaccagtgc tcttgattat gagtcggaaa atataataat
     4921 gaaaaatatg tcaagaatat gtaagaacag aaccgtaatt attattgcac acaggttgtc
     4981 aactgtaaaa aatgcaaaca gaataattgt tatggataat ggctttattt ctgaagatgg
     5041 cacacataaa gagcttatct ccaaaaaaga cagtttatat gcatatttat atcagttgca
     5101 ggcataacgt atgcgctttt acatgaaagg tttatgggat ttggtttgcc gttataagac
     5161 ggttttttct gatgtctgga aaattcgtca cacacttgat gctcctgtga gagaaaaaga
     5221 tgagtatgca tttcttcctg ctcaccttga actcattgaa acaccagtat ccagacgttc
     5281 tcattttgtt gtatggagta ttttattatt tgtaattata tctcttctct tatctgttct
     5341 tgggaaagtt gaagtggttt cagtagcaaa tggtaagttt actcatagtg gaagaagcaa
     5401 agaaataaaa ccgatagaga atgcgattgt agaaaaaata atggtaaaag atggctcttt
     5461 tgtaaaaaaa aatgatccat tagttgaatt gacggtgcct ggtgttgaat ctgatatttt
     5521 aaaatcagaa gcatctttgt tgtatgaaaa aacagaacaa tatcgctacg caattctttc
     5581 tgaatcaata caacggaatg agcttcctga aataaggata acagattttc ctggcggaga
     5641 agataatgcg ggaggtgaac attttcagag ggttagctca ttaataaaag agcaattcat
     5701 gacatggcag aacagaaaaa atcaaaagca gctcacatta aataaaaaaa tagttgaacg
     5761 ggatgcagcg cttgctcgtg ttagcctgta tgagcatcag gtatcacagg aaggaagaaa
     5821 actcaatgat tttaagtatt tgttgaataa aaaagctgtt tctcaacatt cagttatgga
     5881 gcaggaaaat agctatattc aggcaaaaaa tgaacatgca gtctggcttg cacaggtttc
     5941 tcaacttgaa aaagaaatag aacttgtgcg ggaagaactg gcactggaga cgaatatctt
     6001 cagaagtgaa attatcgaga agcacagaaa atcaacagat aacattgtgt tgctggagca
     6061 tgagcttgaa aaaaacaggc agagaaaagc atcatctttt attaaagctc ctgtgagtgg
     6121 tactgttcag gagttaaata tacatacaga aggtggtgtt gtcacaacag cagaaacgct
     6181 gatgattatt gtccctgata atgatattct cgaagtaaca gcatctgtac ttaacaagga
     6241 tatcggtttt atacaacctg gacaggaggt cgttattaaa gtagatgcat atccatatac
     6301 acgtcatggt taccttacag ggaaagtaaa aaatataact gcagattctg tttctgttcc
     6361 ggatacaggg cttgtattta acgtgattat atctgttgat cggaatgata tacagggaga
     6421 aagaaaaaaa atccctgtta cagctggaat gactgttatg gcagaaataa aaaccggggt
     6481 gcgtagtgtt atcagctatc ttcttagtcc attaaaggaa actattaatg aaagtttacg
     6541 tgaacgtta
//
LOCUS       DAZKIF010000201         6495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZKIF010000201 DAZKIF010000000
VERSION     DAZKIF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.202"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(220..2253)
                     /gene="betT"
                     /locus_tag="ACHOTW_004434"
     CDS             complement(220..2253)
                     /gene="betT"
                     /locus_tag="ACHOTW_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP9846409.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2382..2969
                     /gene="betI"
                     /locus_tag="ACHOTW_004435"
     CDS             2382..2969
                     /gene="betI"
                     /locus_tag="ACHOTW_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP9846410.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2983..4455
                     /gene="betB"
                     /locus_tag="ACHOTW_004436"
     CDS             2983..4455
                     /gene="betB"
                     /locus_tag="ACHOTW_004436"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP9846411.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4469..6157
                     /gene="betA"
                     /locus_tag="ACHOTW_004437"
     CDS             4469..6157
                     /gene="betA"
                     /locus_tag="ACHOTW_004437"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP9846412.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1608 a         1791 c         1771 g         1325 t
ORIGIN      
        1 gaatcgtctt attaataaat atataaccct ataggactga atccattcag ctaatacaaa
       61 ataaaatcgt taattaataa aatataaaat catgaagtga tacaagagga atttcaggat
      121 atgacatgac aatcatatat aatatatgca taatttttta cagcgagtca atgattcccg
      181 cgccatatgc agacatggcg cggttttatg caataacaat cacgcgtccg ggaacatcac
      241 gctatggccc ggcgcttcac gatggagatg aataaagtta aggtgccgct cgtactggtc
      301 aagaatatcg gtgatcacct gctctttgct gtagtccatc aggtcgttgc cctggctgcc
      361 ttctaacagg aaggtttcca gccggtagta ggtcgatttc ccgctacgtg cgcggtaggt
      421 aaagcccggc accgagtatt gctgcggcca aatctgatag acaaagtttt gctcttcgcc
      481 catatgcacc aacaaatcca gatgccccaa ctgttgtccc tcttccggcg gcaggctttt
      541 tagctccacg tacgcgccgc gcaaccgcaa ctcctgcgcc acttcttcca ttgccgggta
      601 acagaccgtc tccatcatct gtttagtgta acgcgtgccc ggataattca tcaggcgcga
      661 gagacgtttt ttccagctca ggcgatcctg aagccccagc ggtcgcggtg cggtatcgcg
      721 gttggcactt tcacggcggt aatcttctac cttcagagat ttatacaacc ccgccatcac
      781 gaagaagatc acaaagctga acggcagccc cataatcacc gtggtgtttt gcagcgcgga
      841 tatcccgttg gtcatcagca tgccgagcgt cagcaggcca atcgccaccg accagaagac
      901 gcgcagccag ccgggggcgt cgctgttgat atctttaagc tgcgaggtga aattccccag
      961 caccagcgcc ccggagtccg ccgaggtcac ataaaacagc aggccagtaa tggtggcgac
     1021 ggaggcgcta aaggtaaacg ccggatactg cgccagcagg ctgtagaagc cgcgctccgg
     1081 atggaccatc gcttcctcgg caaatgccgc gccgccgtgg atgatttcat acagcgcgct
     1141 attgccgaac accgagagcc ataacagcgt gaaggtaaac ggaataatca acgtgcccag
     1201 cacgaactgg cgaatggtac gcccacgcga gatacgcgcc aggaacaagc cgacaaacgg
     1261 cgaccatgcc acccaccatg cccagaagaa gagcgtccag ttattcatcc actcaaccgg
     1321 gcggtcgaag gcaaaactgt tgagcgtcat gcccataaag cgattcacat agtcgccaac
     1381 attcagcacc agtgcattaa gcaggaacga agtgtcgccc ataaacaaca cgaacaggat
     1441 caatcccagc gccagcgcga cattaagctc cgataacacg cgaatgccct tatcgacacc
     1501 ggatgtcacc gagatagtgg cgattatcac cgacaaggcg atcagtgccg cttttgccgc
     1561 catcgaatcg ggaatatcaa acagtacgct caagccatag ttaagctgca ccacaccgat
     1621 accgagcgta gtggcaatac cgaagatagt gccgatcacc gctgcaatat ccactgagtg
     1681 acctatcggc ccgttaatcc gtttaccgaa gatcgggtac agcgccgagc ggatggtgag
     1741 cggcaaatta taacgatagc taaagtatcc gagcgccatg cccatcagcg catacatcga
     1801 ccagccggtt aagccgtagt gaaacagcgt ccagaccatc gcctgacgcg cggcctcaat
     1861 cgtctgtccc gcgccttccg gcggctgcat atactgcgtt accggttcgg ctacggaaaa
     1921 gaacatcaga tcgataccga tcccggcagc aaacagcatc gccgcccaac tcagcaggct
     1981 gaattccggt ttggattgtt ctggcccgag cttcaccgaa ccaaaacgcg aacaagcgat
     2041 acagaccaca aagacaatat agagcgttgc cgccagcaga tagtaccaac cgaaggtttt
     2101 agaaacccag tccagcgtgc ggccaatcca cagggccgag aagtcgcgaa acaggatcgt
     2161 tgtcagggaa aacaacaaaa tcagtccggc ggaggtgtaa aacaccaccg gattgatttt
     2221 gtccttttcc ctgctgtgtg aaaggtctgt catccagtat ccccactgtt attgttacca
     2281 tttaaaatca aattcgtaac aattaagaca cattttatat tgaacgtcca atcaataacc
     2341 gctttaatag ataaacaacg ctgatgaatg gagtggcaaa aatgcccaaa ttggggatgc
     2401 agtcgatccg gcgcagacaa ctgatcgacg ccacactgga agcaataaat gaagtgggca
     2461 tgcacgatgc aacgatcgcg cagatcgccc gccgtgcagg cgtttctacg gggatcatca
     2521 gccactattt cagggacaaa aatggtctgc tggaagcaac catgcgcgat atcaccagtc
     2581 agctgcgtga cgcggttttg aatcgattac atgcacttcc gcagggcagt gcagagcagc
     2641 gattacaggc gattgttggc ggaaacttcg atgaaacgca ggtgagcagt gcggcgatga
     2701 aagcctggct ggcgttctgg gccagcagta tgcatcagcc gatgctctat cgtttacagc
     2761 aggtcagcag ccgccgcttg ctgtcgaatc tggtgagcga gtttcgccgc gaattgccgc
     2821 gccaccaggc acaggaagcg ggctacggcc ttgccgcgct gattgacgga ttatggctgc
     2881 gcgcggcact gagcggcaaa ccgctggata aacctctcgc acactcgctg acccgtcact
     2941 ttattactca gcatttaccc accgattaac cgaggagacg tgatgtcccg aatggcagaa
     3001 cagcagcttt atatacatgg tggttatacc tccgccacca gcggtcgcac cttcgagacc
     3061 attaacccgg ccaacggtaa cgtgctggcg accgtgcagg ccgccgggcg cgaggatgtc
     3121 gatcgcgccg tgaagagtgc ccaacagggg caaaaaatct gggcggcgat gaccgctatg
     3181 gaacgctcgc gtattctgcg tcgggccgtc gatattctgc gcgagcgcaa tgatgaactc
     3241 gccaaactgg aaaccctcga caccggaaaa gcgtattcgg aaacctcaac cgtcgatatc
     3301 gttaccggtg cggacgtgct ggagtactac gccgggctga tcccgtcgct ggaaggcagc
     3361 cagatcccgt tgcgtgagac atcatttgtc tacactcgcc gcgaaccgct gggcgtggtg
     3421 gcagggattg gcgcatggaa ctacccgatc cagattgccc tgtggaaatc cgccccggcg
     3481 ctggcggcgg gtaatgcgat gattttcaaa ccgagcgaag tcactccgct taccgcgtta
     3541 aagctggctg aaatttacag cgaagcgggc ctgccggacg gtgtatttaa cgtgttgccg
     3601 ggcgtgggcg cggagaccgg gcaatatctg accgagcatc cgggcattgc caaagtgtcg
     3661 tttaccggcg gcgtcgccag cggcaaaaaa gtgatggcta actcggcagc ctcttccctg
     3721 aaagaagtga ccatggaact gggcggtaaa tcaccgctga tcgttttcga cgatgcggat
     3781 ctcgatctcg ccgccgatat cgccatgatg gcaaacttct tcagctccgg tcaggtgtgt
     3841 accaatggca cccgcgtctt cgttccggcg aaatgcaacg ccgcatttga gcagaaaatt
     3901 ctggcgcgcg ttgagcgcat tcgcgcgggc gacgttttcg atccgcaaac taacttcggc
     3961 ccgctggtca gcttcccgca tcgcgataac gtgctgcgct atatcgccaa aggcaaagag
     4021 gaaggcgcgc gcgtactgtg cggcggcgat gtactgaaag gcgatggcct cgataacggc
     4081 gcatgggttg caccgaccgt gttcaccgat tgcagcgacg agatgaccat cgtgcgtgaa
     4141 gagatcttcg ggccggtgat gtccattctg acctacgagt cggaagacga agtcattcgc
     4201 cgcgccaatg ataccgacta cggcctggcg gcgggtatcg tgacggcgga cctgaaccgc
     4261 gcgcatcgcg tcattcatca gctggaagcg ggtatttgct ggatcaacac ctggggcgaa
     4321 tccccggcag agatgcccgt tggcggctac aaacactccg gtattggccg cgagaacggc
     4381 gtgatgacgc tccagagtta cacccaggtg aagtccatcc aggttgagat ggctaaattc
     4441 cagtccatat tctaaccagg aggtttattt gcaatttgac tacatcatta ttggtgccgg
     4501 ctcagccggc aacgttctcg ctacccgtct gactgaagat ccgaatacct ccgtgttgct
     4561 gcttgaagcg ggcggcccgg actatcgctt tgacttccgc acccagatgc ccgctgccct
     4621 ggcattcccg ctacagggta aacgctacaa ctgggcttac gagacggaac ctgaaccgtt
     4681 tatgaataac cgtcgcatgg agtgcggacg cggtaaaggc ctgggtggat cgtcgctgat
     4741 caacggcatg tgctatatcc gtggcaacgc gatggatctc gacaactggg caaaagaacc
     4801 cggtctggag aactggagtt atctcgattg cctgccctac taccgcaagg ccgagacccg
     4861 cgacgtgggc gagaacgact accacggcgg cgacggcccg gtgagcgtca ccacctccaa
     4921 acccggcgtc aatccgctgt ttgaagcgat gattgaagcg ggcgtgcagg cgggctaccc
     4981 gcgcacggac gatctcaacg gttatcagca ggaaggtttc ggcccgatgg atcgcaccgt
     5041 cacgccgcag ggccgccgcg ccagcaccgc gcgcggttat ctcgatcagg ccaaatcgcg
     5101 cccaaaccta accattcgta ctcacgccat gaccgatcac attatttttg actgtaaacg
     5161 cgcggtgggc gtcgagtggc tggaaggcga cagcaccatt ccgacccgcg cgacggcgaa
     5221 caaagaagtg ctgttatgtg caggcgcgat tgcctcaccg cagatcctgc aacgctctgg
     5281 cgtcggcaac gctgaactgt tggccgagtt tgatattccg ctggtgcatg atttacccgg
     5341 cgtcggtgaa aatcttcagg atcatctgga gatgtatctg caatatgagt gcaaagaacc
     5401 ggtttccctc taccctgccc tgcagtggtg gaatcagccg aaaatcggtg cggagtggct
     5461 gtttggcggc accggcgttg gtgccagcaa ccactttgaa gcaggcggat ttattcgcag
     5521 ccgagaggaa tttgcgtggc cgaatattca gtatcacttc ctgccggtag cgattaacta
     5581 taacggctcg aatgcagtga aagagcacgg cttccagtgc cacgtcggct cgatgcgctc
     5641 gccaagccgt gggcatgtgc ggattaaatc ccgcgacccg caccagcatc cggcaattct
     5701 gtttaactac atgtcgcacg aacaggactg gcaggaattc cgcgacgcaa ttcgcatcac
     5761 ccgcgagatc atgcatcaac ccgcgctgga tcagtatcgt ggccgcgaaa tcagccccgg
     5821 cacggaatgt cagacggatg agcagctcga tgagtttgtg cgtaaccacg ccgaaaccgc
     5881 cttccatccg tgcggtacct gcaaaatggg ctacgacgag atgtccgtgg ttgacggcga
     5941 aggccgcgtg catgggctgg aaggcctgcg cgtggtggat gcgtcaatta tgccgcagat
     6001 tatcaccggg aatttgaacg ccacgacgat tatgattggc gagaaaatgg cggatatgat
     6061 tcgcgggaag gaagcgttgc cgaggagcac ggcgggatat tttgtggcaa atgggatgcc
     6121 agtaagagcg aaaaaaatga gtcgtgattt gaactgactc aggaaccgat cagattgaga
     6181 gttaccgttc caggtatggg gacagaatcg ttatataaaa cataggggta actctcatgc
     6241 ttcatagtga caacctcgcc agcaaattct aagctgactc gttcaatctc gctgatgaag
     6301 gtactatgaa tgcactgata aaccgcaggt gaaatgccac tcagcttaag aattacgccg
     6361 ctagtgttcc tggctatggt gacttgttca ctcccgctca cggttagcat atacgaaaag
     6421 ggttcttgta acgaggacaa aaataagtca gaccaaaaca acgtggagct ttaaacacaa
     6481 ttcgtataat ttatt
//
LOCUS       DAZKIF010000202         6486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZKIF010000202 DAZKIF010000000
VERSION     DAZKIF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.203"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..771)
                     /locus_tag="ACHOTW_004438"
     CDS             complement(<1..771)
                     /locus_tag="ACHOTW_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP9846413.1"
                     /translation="MQKRVLPIDVYSDETDEPRLIVRKKEHDLYKYYILQDSKDYKPE
                     KPSDTELVFISNAETIRDYLLRLSVDELKLLAKYILQNVYIVFVQTDDFASSFRLFNV
                     LNSRGLPLSNADLLKNALFESASTHNKKSEQIESAWSQIEDMVGVRRLDKFLTLHKLS
                     EKKDRDRVLQKGFEAFIENLQQQFDGDAIAMSLMLVNSAKNYTKILENDFEHPSIRRK
                     IASLSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFIT"
     gene            complement(780..1064)
                     /locus_tag="ACHOTW_004439"
     CDS             complement(780..1064)
                     /locus_tag="ACHOTW_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP9846414.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENGYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKSSRFRG"
     gene            1342..3774
                     /gene="hsdR"
                     /locus_tag="ACHOTW_004440"
     CDS             1342..3774
                     /gene="hsdR"
                     /locus_tag="ACHOTW_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFP9846415.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            3841..5310
                     /locus_tag="ACHOTW_004441"
     CDS             3841..5310
                     /locus_tag="ACHOTW_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9846416.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            5310..>6486
                     /locus_tag="ACHOTW_004442"
     CDS             5310..>6486
                     /locus_tag="ACHOTW_004442"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9846417.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
                     QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFV
                     CYVENCQTGVAYPAINDKQFFSGITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSL
                     DAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASVDALKQTILQLAVMG
                     KLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCL
                     "
BASE COUNT         1934 a         1510 c         1439 g         1603 t
ORIGIN      
        1 ggtaataaat tgagaaaaat catctaaatt aaaatcttct gtacgagcca ttcgattcat
       61 gaatgccata accggaggaa tccactcatc tacccctaaa ttacttagcg atgctatttt
      121 tcgtcgtatt gaagggtgtt caaaatcatt ctctaaaatc ttagtataat tttttgcaga
      181 attaaccaac atcagtgaca tagcaatagc atctccatca aattgctgct ggagattttc
      241 aataaaagct tcaaaacctt tttgtaacac cctgtcacga tcttttttct cggaaagctt
      301 atgaagagta agaaatttat caagccgtct cactccgacc atatcttcaa tttgcgacca
      361 tgctgattct atttgctctg attttttatt atgtgtagaa gctgattcaa acagtgcatt
      421 ttttaaaaga tctgcattac ttaaaggtaa acctcgacta tttaatacat taaagagtct
      481 aaatgatgat gcgaaatcat ctgtttgcac aaatacgata tatacatttt gcaaaatata
      541 tttagcaagc aacttcaact catcaacaga taaccgcaac aaataatctc taattgtttc
      601 agcgttgcta atgaatacta actcagtatc cgatggcttt tcaggtttat aatctttaga
      661 atcctgcagt atataatatt tataaagatc atgctctttt tttctaacaa ttagtcttgg
      721 ttcatctgtt tcatcagaat aaacatcaat aggcagaact cttttttgca aatcatcttt
      781 tatcccctga atcgggatga tttttttagc tcggagacaa taatgcttaa cgttgtaaga
      841 cgttgttgtc catctacaac ttcatactga ttttgccctt tattgataca aatcatactc
      901 ccaataaaat atccattctc ttctgattga gagcttttat aaatgtcatc aatgagttgc
      961 tctgcattat ctaccgtcca actgtaagga cgctgataag aaggaataat aaactttttg
     1021 ttttcagtaa ggatatcctg caccttacat tctttagctt ccatttcata tacccaatca
     1081 aacaatcaac aataacatta tctttcttgc tcgaaagaaa cgcaactata agatttttat
     1141 atattttgtt agagttgata tggttcctgt aacttctgtt tatctgtctc tgatatgccc
     1201 agattaattc aagcaagata taacagagat atcaatgtga tctctgctac gtactttacg
     1261 ttgaaacacc gccctaaatc atagccaggc tattgatttt atgtacaatc agaatcgtcc
     1321 tgataaaagc gagaataccc catggcggaa ctgaacctaa gtaacctgac ggaagcagac
     1381 atcattacca aatgcgttat gccagccatt ctcaatgcag gctgggacaa cacaacgcaa
     1441 atcagacagg aggtcaaact ccgggacggt aaagtcatcg tacgtggtaa agttgcggca
     1501 cgcagaacgg taaaatctgc tgacatcgtg ctgtatcaca aacctggcat tcccttagcc
     1561 gtgattgaag caaaagccaa caaacatgaa attggcaaag ggatgcaaca gggcattgaa
     1621 tatgcgcgcc tgctggacgt tccttttgtt tttgccacca acggtgatgg ctttatcttc
     1681 cgcgatgcca ccgcagccga aggtgaatgc cttgaaaagc aaataacgct ggatgacttc
     1741 ccctcccccg ctgaactctg gcaaaaattc tgcctctgga aaggatacac acaagctcag
     1801 cttccggtga ttactcagga ctattacgac gatggcagcg gtaaatcgcc acgttattac
     1861 cagcttcagg caatcaacaa aaccattgaa gccgtctcca acgggcaaaa ccgcgttctg
     1921 ctggtcatgg cgaccggaac agggaaaacc tataccgcat tccagatcat ctggcgcctg
     1981 tggaaatcaa aaaataaaaa acgcattttg ttccttgccg atcgcaatat tctggtcgac
     2041 caaaccaaaa ataatgattt ccagccattt ggtacggcaa tgaccaaagt cagcggacgc
     2101 accattgatc ccgcttatga aattcacctc gcgctctatc aggctataac tggcccggag
     2161 gaagaccaaa aagcgtttaa acaagtcgca ccagatttct tcgatctgat cgtgatcgac
     2221 gaatgccatc gcggcagcgc atccgaagac agcgcctggc gagaaatcct tgattatttt
     2281 agttccgcca cccaaattgg cttaaccgcc acgccaaaag agacgcatga agtctccagc
     2341 acggattact tcggcgatcc ggtttacgtc tactcactaa aagaagggat cgaagacggc
     2401 ttcctcgccc cttataaagt tgtccgtgtt gatattgatg ttgatctgca aggctggcgc
     2461 ccaaccaaag ggcaaactga cttaaacggc gaagtgatcg acgatcgtat ctataaccag
     2521 aaagatttcg atcgcacgat ggtaatcgac gaacgcacag aactggttgc cagaaccatt
     2581 accgactacc tcaagcgtac caatccgatg gataaaacca tcgtcttctg taacgacatc
     2641 gatcatgcag aacgtatgcg ccgcgccctg gttaatctca acccggagca ggtgaaaaag
     2701 aacgacaaat acgtcatgaa aatcaccggc gatgatgaaa ttggcaaagc tcagttggat
     2761 aacttcatca acccgaaaaa accgtacccg gttatcgcga ccacttcaga gctgatgacc
     2821 accggtgtgg atgctaaaac ctgcaaactg gtagtactgg accagaacat ccagtcgatg
     2881 accaaattca agcagattat cggtcgtggt acacgcatcg acgaacgtta cggcaaactc
     2941 tggtttacca tcctcgactt taaaaaagcc accgaactgt ttgccgatga gcgtttcgat
     3001 ggcattcccg aaaaagtcat ggataccaca ccagaggata tcgccgatcc agaatctgat
     3061 tttgaagaga aactcgaaga aatcagcgaa catgacgacg aacaggtaac aggcgttgat
     3121 gaaccgcctg cgccaccata ccaggttaaa gataccgatg atgtcggccc acttccggaa
     3181 gaagacgaga agaaaatccg caagtttcac gtcaacggtg tagcagtggg cgttattgcc
     3241 cagcgtgttc agtattacga cgccgacggt aaactggtta ccgaatcctt taaagattac
     3301 acccgcaaaa cactgctcaa agaatatgcc tcgctggatg actttacccg caagtggcag
     3361 gacgccgatc gcaaagaagc gatcattcac gagctggagc aacaggggat catctgggaa
     3421 gtactggcag aagaagtcgg taaagatctc gacccgttcg acatgctttg ccacgtagtg
     3481 tatggtcagc cgccgttaac ccgcaaagag cgcgccgaga acgtgcgcaa gcggaactac
     3541 ttcacaaaat actctgaagc agcgcaagcc gtgctcgata atctgctgga taaatacgcc
     3601 gatgcgggcg tacaggagat cgaaagtatt caggtgctga aacttaagcc attcgacagc
     3661 atgggcacct taccggagat tattaaaacc ggatttggcg accgtaacgg gtataatcag
     3721 gcgctcagcg agctggaaaa cgaaatctac caattaccgc cccgctctgc ttaacaactc
     3781 tcttttgcgc cttctgcaac catcatgttg cagaaggcct tgtttttcat ggaatacaac
     3841 atgtctatca gctcagtaat caaatcatta caagatatta tgcgcaaaga tgccggtgtg
     3901 gatggcgatg cgcagcgtct cggtcagctc tcctggctgc tgtttttgaa aatcttcgat
     3961 gcccaggaag aggcgctgga actggagcag gataactatc aatatccgat cccacagcgt
     4021 tatttatggc gcagttgggc cgccaacgcg cagggcatta ccggtgattc cctgctggaa
     4081 ttcgttaatg atgatctgtt cccggcgttg aaaaacctca ctgcgcctat cgataaaaac
     4141 ccacgcggct acgtggtgaa gcaggcgttc agcgatgcct ataactatat gaaaaacggt
     4201 acgctactgc gtcaggtgat caataagctg aacgaaattg actttaccag cgccagcgaa
     4261 cgccatctgt ttggtgatat ttacgaacag atccttaaag atctgcaatc tgcgggcaat
     4321 gcgggcgaat tctatactcc acgcgccgtc actcgcttta tggtggatcg cgttgatccg
     4381 aaactcggcg aatccattat ggacccggcc tgcggtacgg gcggttttct tgcctgcgca
     4441 tttgatcatg taaagaacaa atacgtgaag agcgtcgccg atcatcagac gctgcaacaa
     4501 cagatccacg gtgttgagaa aaaacagctt ccgcacctgc tggcgaccac caatatgctg
     4561 ctacacggca ttgaagtgcc agtgcaaatt cgtcacgaca acactctgaa caaaccgctt
     4621 tcctcccggg atgagcaact ggatgtcatt gttaccaacc cgccgtttgg tggcacggaa
     4681 gaagacggta ttgagaagaa ctttccggca gagatgcaaa cccgcgaaac ggcggatttg
     4741 ttcctgcaac tgattgtgga agtactggcg aaaaatggtc gcgcggcggt ggtattgccg
     4801 gatggcacac tatttggcga aggcgttaaa accaaaatca aaaagctact taccgaagag
     4861 tgcaacctgc ataccatcgt gcgcttaccg aatggtgtgt ttaaccccta taccggcatt
     4921 aaaaccaacc tgttgttctt taccaaaggt cagccaacca aagagatttg gttctatgag
     4981 catccgtatc cggcgggcgt aaaaaactac agcaaaacta agccgatgaa gtttgaagag
     5041 tttcaggcgg agatcgactg gtggggtaac gaggccgatg gttttgccag ccgcgtagag
     5101 aatgagcagg cgtggaaagt cagcattgat gacgtgattg cgcgtaactt caatctggat
     5161 attaaaaacc cacatcaggc ggaaaccgtc agccatgatc cggccgagct gttagcgcag
     5221 tatgcaaaac agcaggcgga gatccagacg ctgcgtaatc aactgcgcga tattcttggc
     5281 gctgcgctgt ctggcaagga ggttaactaa tgagtgtgga aaagctgatc gttgatcata
     5341 tggaaacctg gacctcggcg ttgcaaaccc gttccaccgc cgggcgcggc agttccggaa
     5401 aaattgattt atatggcatt aagaaattac gtgagctgat tctggaactg gctgtgcgcg
     5461 gtaaactggt gccgcaggac ccgaacgatg aaccggcgtc ggagctgctg aagcgtattg
     5521 cggcagaaaa agcagagctg gtgaagcaag gtaaaattaa aaagcaaaaa ccactgccgg
     5581 aaattagtga ggaagagaag ccgtttgaat tgccggaggg atgggagtgg gtgcgtatta
     5641 gtgaaattgg acatgactgg ggacaaaaaa ctccagataa agattttact tacattgatg
     5701 ttggttcaat aaataaagaa tatggaatta ttgaagagct atctattctt tcagcgaaag
     5761 atgccccatc gcgagcgaga aaaattgttc agcaagggac tatcatatac tctactgttc
     5821 gcccgtattt gttaaatata gctattattg aaaacgaaat actacctgaa ccgatcgcta
     5881 gtactgcctt cgctattatt catccatata cggcgatgga tgctaatttc atttattatt
     5941 atctacgttc tccggtattt gtttgttatg ttgaaaattg tcagacaggg gttgcctatc
     6001 cagcaatcaa tgacaaacaa tttttttctg gaataactcc agttcctcca tccttggaac
     6061 aagttcgcat cgcaaacaaa atcaaagaat taatgtccct ctgcgaccaa ctggaacagc
     6121 aatccctgac cagtctggac gcacatcaac aactggttga aaccctgttg ggaacgctta
     6181 cagacagcca aaccgccgag gaactggctg aaaactgggc gcgtattagc gagtatttcg
     6241 acactctatt taccaccgaa gccagcgtgg atgcgttaaa acagaccatt ctgcaactgg
     6301 ccgtaatggg caaacttgtg ccgcaggatc cgaatgatga accagcctct gaactgctca
     6361 aacgaattgc gcaggaaaaa gctcaactgg tgaaagaagg aaaaataaaa aaacaaaaac
     6421 cgttgccgcc aattagcgat gaggaaaaac cgtttgaact tccggaaggg tgggagtggt
     6481 gtttat
//
LOCUS       DAZKIF010000203         6154 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZKIF010000203 DAZKIF010000000
VERSION     DAZKIF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.204"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            108..242
                     /locus_tag="ACHOTW_004443"
     CDS             108..242
                     /locus_tag="ACHOTW_004443"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846418.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            843..1664
                     /locus_tag="ACHOTW_004444"
     CDS             843..1664
                     /locus_tag="ACHOTW_004444"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9846419.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1664..2770
                     /locus_tag="ACHOTW_004445"
     CDS             1664..2770
                     /locus_tag="ACHOTW_004445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9846420.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            2860..4581
                     /gene="cptA"
                     /locus_tag="ACHOTW_004446"
     CDS             2860..4581
                     /gene="cptA"
                     /locus_tag="ACHOTW_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9846421.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4655..5653
                     /gene="lpxM"
                     /locus_tag="ACHOTW_004447"
                     /gene_synonym="msbB"
     CDS             4655..5653
                     /gene="lpxM"
                     /locus_tag="ACHOTW_004447"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9846422.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            5706..5894
                     /locus_tag="ACHOTW_004448"
                     /pseudo
     CDS             5706..5894
                     /locus_tag="ACHOTW_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5998..6084)
                     /locus_tag="ACHOTW_004449"
                     /pseudo
     CDS             complement(5998..6084)
                     /locus_tag="ACHOTW_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1575 a         1350 c         1545 g         1684 t
ORIGIN      
        1 cagcttttta aactaatatc gactttcaat aaataaggac aggtaatgga caacaaaaat
       61 atctgaaccc gggtgagatt cataagttgc acagtgctgt cttcaaagtg ccacacccgg
      121 aacgtaatca ctgcctgctc cttatggggt atcttcatgg tgtccaagca tcagagttat
      181 taggtataaa actgtctgat attgatttgc aggccggtaa cctgaatatc cggcggctct
      241 aaacggtttg tcaactcagc ccctcggctg aaacaggagg tacagtccat tcgtcgctgg
      301 ctgaaacttc gggatgaatg ggtagaagag ggatatgaat ggttatttaa cctctatggc
      361 aggtctgcta caggtattac tgttgttctg gggggcactg gtgtggttaa tatctacgat
      421 gcaagagatt ctcacgatgg cccggtccat cagtgcatgc ggcgcgatat tgtgcgagta
      481 tttttcaggg ggagtggtat ccggatgcag aaaattccgc tactcgactg gttttatgca
      541 ataaatcagg atgataatct tctgattttt tactgaggcg gatatctctc gctgtgttta
      601 ctgaaattgc ggtattctgt tttttctgaa aaacagaaaa tctgattaaa aagagcatat
      661 gcgttaaggg aaatttaagt tctggttgat taactgataa aaatattaaa aaatccctgt
      721 tgtcggtaat ctccggacca ttccgggatt acacaataat atcaccatgt gtgatatcta
      781 tggatcctca ctgttgttta cgcgaggata ttttcagaat gcttaacaga cgattatttt
      841 ttatgttgaa tgcaaggcat cttcccgtac tgatgtatca tcatgtcagt cgttgtccgg
      901 ggcttgtgac actctcacct gaaacatttt gtgaacagat gaagtggctc gcagataata
      961 actggcggac ggtaacatct gctgagcttg aatattttta tcagggggga acgcttccac
     1021 gaaagagtgt catgctcaca ttcgatgatg gttatcttga taactggctc agagcatggc
     1081 cggtcctgaa ggaatataac cttcatgcac atatttttct cattacgggg ctgattggta
     1141 agggaaacgt acgtagcagg caggagcagg aatattctca ccgcgactgt gaacggttga
     1201 ttgccgataa ccgttcagat gaggtcatgc ttcgctggtc tgaagtccgg gaaatgcgtg
     1261 attccggact ggttgaattt catgtgcaca cccattcaca taaacgctgg gacaggttgt
     1321 ctgtatcacg tgctgaacag tgccggctca tgaaagaaga tattctggtg ggaaaacaat
     1381 gtctgacgga aaagctgggg ttctgtagtt cccatctgtg ctggcctgaa ggatattata
     1441 atcgggatta tattaatctc gccggtaagc ttggtttctc ttatttatat acaaccgaaa
     1501 gaagaatgaa ctgtcctgaa aatggttcat tacgcatagg gcgtatcagc accaaagagc
     1561 gggaacattc cggctggctg aaacgacgcc ttttttatta tacaacgcca cttttttctt
     1621 cagtactggc tcttcataag gggccaagat taccagataa ttaatgatga aaattttatt
     1681 cactgaatct tcgtcagata ttgggggcca ggagctgcag gcactggccc agatgactgc
     1741 tcttcaaaag cagggacatt ccgttttact tgcctgcaga gaaaaaagta aaattgctcc
     1801 ggaagccaga aaaagagggc atgatgtcac ttttattcct ttcaggaaca gccttcacct
     1861 tccttccatc ctcagacttc gacggattat cggggaattt aaacctgatc tggtcatctg
     1921 ccatagtggc catgacagta atatagcagg actgagccgt ctgatatgct gtcaccgctt
     1981 cagtattgtc agacaaaaaa cctacatcac ccgtaagacc agaacgtttt ccctgaatta
     2041 tctgtgtgat ttcattgttg tgcctagctc agccatgatg gcgcatctga tggcggaagg
     2101 tgtcaggaca ccggtaacgg ttattccacc gggtttcgac tggcccgcat tgcataatga
     2161 agccatgcga ccgttacccc tgcatatcca cgcctgggct gcatcagcag acaatgttcc
     2221 gcttatcgtt caggtgggga tgctacgtcc cgaaaaagga catgaattta tgctgcgtgt
     2281 attgtatcag ctgaagatgg aagggaaatc atttcgctgg ctcgtggtgg gcgccgggcg
     2341 tgaagaatac gaagcacgtc tgcgtcagca gacagaacac ctgggaatga gcggagatgt
     2401 gttaatggca ggtgcccttt tcccagcgtt accggtctac cggattgcca gtgtggtggt
     2461 catgccttcg gaaaatgaag cgtttggtat ggtactggct gaagcgtcag tctccggcgt
     2521 gcccgttatc gccagtgaga cgggcgggat ccctgatgtc attcagaaga atgtgacagg
     2581 gacgctgtta cccgtggggg atgtgtctgc atggaccggc gcactgcgcg attttttatc
     2641 ccgaccggaa cgcttccgga tgatggcagc cagcgccaga gaagatattg agtaccggtt
     2701 tgatattaac agaacggcac agatcattgt gtctctggct agtcaggcta aggggaaatg
     2761 caacagataa cggcggatcc tcttttcagc ccgaattttg ttcagcggtt ttccggtaac
     2821 ggagtaattt atcacattgt ttaaatacgg ggacggtcaa tgcatttaaa taccggacag
     2881 aacaggccta ctttcagctg gagcgcgctg ggatgggcaa ttttttattt tggctttttt
     2941 tccactctcc tgcaggtcat catcttcagc agtgggtaca gcggaacgaa tggaatacgg
     3001 gactcactgt tattcagttg tctgtggttg atcccggtgt ttctctatcc tgatcggata
     3061 aaaataattg cagctgttgt cggtttcatt ctctggggca cgtcgctggc agcactttgt
     3121 tattattttc tctatggtca tgaattctct caaagtgttc ttttcgttat gtttgaaacg
     3181 aatgccagag aggctggtga atatttcagc cagtatttca gccttaaact gttgcttatc
     3241 tcgctggtat atactgcggt gtccgttttt ctgtggacac gtctgcgtcc tgtatatatt
     3301 cctttgccat ggcggagaat tgtctctttc ctgctgcttt atgctctgct tctgcatccg
     3361 gttgttctga aatcgttaat cagacaggag ccgctgaatg atactctagg caaactggca
     3421 tcccgaatgg agcctgctgc cccctggcag tttgtatcca gttattatca gtaccatcag
     3481 caactgaatg cactgacaac cttcctgaat gaaaatagcg cactgccacc actgggtaat
     3541 ctcagggatg aatcagggga gagaccacgc acactggtcc tggtgattgg tgagtcgaca
     3601 cagcgcgaac gcatgagctt atacgggtat ctacgtgaaa cgacgccgga gctggatgca
     3661 ctgcgtaaaa ccgatccggg tcttactgtg tttaataatg tggtggcatc gcgtccgtac
     3721 accattgaag cattgcaaca ggcccttact ttcgccaacg aaaagaatcc tgatctgtat
     3781 ctgacgcagc cgtcgctgat gaacatgatg aagcaggcag gctataaaac attctggatt
     3841 accaaccagc agacaatcac agcccgtaac accatgctca ctgtattttc gcgccagacg
     3901 gacaggcagt actacatgaa tcagcagcga acacaaagtg cgcgtgaata tgacactaac
     3961 gtgctgaagc cgttccggga agtgctgaat gaccctgcac caaagaaact gatcatcgtg
     4021 catctgctgg gtacgcacat taagtataaa taccgttatc cggaaggtca ggggcggttt
     4081 gatggcatta cagggcatat tcccactgga ttaaatgcga aagagctgga agtgtataac
     4141 gattacgata atgccaatct gtttaacgat catgtggtgg ccagtctgat aaaggacttc
     4201 agagcaactg cgccggacgg ctttctgctt tatttttcag accatggtga agaagtatat
     4261 gatactccgc catataaaac gcagggacgg aatgaagata accccacacg tcccatgtac
     4321 actgttccgt tcctgctgtg gacctcggaa aagtggcatg ctgcacatcc gcgagatttt
     4381 tcgcagtatg ttgaccgcaa atacagtctg gctgaactga tccacacctg gtcagatttg
     4441 gcgggactga catacgatgg ttacgatccg acccgttctc tggtgaatcc gcaattcagg
     4501 gaaaccaccc gctggattgg aaatccgtat aaaaagaatg ggctcactga tttcgacact
     4561 cttccgtatg gtgagccata gtattttctt atcttcggtt ccgttctgaa ggcaggagga
     4621 tgtgttttcc tctgctgtcc ggtggcctcc agggatggta ccaccttctg ctgtactctg
     4681 ttatcataat gagataagca ggcagatacc ggtaaatatg aaaaatatca gaactgagtt
     4741 tattccccgc tttaacctta cattatgctt cccccgttac tggatgacgt ggacaggtat
     4801 cggtattatc tgtgtgtttg caatggtccc ccccgcactg agagatccat tgttgggaaa
     4861 attgggcatg ctggttgggc gtctggggaa aagcgcacgt caacgggcct tgattaattt
     4921 atcgctttgt tttccggagt acagtgataa agaaaaggag aatatcgttg atgccatgtt
     4981 tgcaacagcc tccatggccg ttgtgcttat ggctgaatta gcactgtccg ggcctgataa
     5041 aatttcgcat cgtatacgct ggaatggtct ggagattgtg gagaagatgg cacagaacaa
     5101 tgaaaaggtc atttttcttg tgccacatgc ctggggagtg gatattcccg caatgctgat
     5161 ggctgcgtcg ggcagaaaaa tggcagcgat gtttcataac cagagaaatc cggtggtgga
     5221 ttatgtctgg aacagtgttc gtcgtcgttt cggaggaaaa ctgcacgcac gtaatgacgg
     5281 tattgcctcc tttgtccgct cagtccggca gggatactgg ggatattatc ttcctgatca
     5341 ggatcatggc ccggaattca gtgagtttgc agattttttt gcgacgtata aagcaacttt
     5401 accggttatc ggacggctca gcaggatcag tggtgcacgg ataataccgc ttttccctgt
     5461 ttatgacgga aagacccatc atctgactat tcatgtcagc ccgccacttg ctatacgaca
     5521 gaagagtgat gctcatattg cgcgacagat aaacgaagtg gttgaaaatt tcgttcgtcc
     5581 acatcctgag cagtatacgt ggatcctcaa gttgctgaaa acgcgtaaag aaggggaaga
     5641 ggatccttac tgaggcagct ttttcaggat aatgagctta cccagcaata gtggacacac
     5701 gctaagtgag taaactctca gtcagaggtg gctcatatga caaaaaaaca gtaccaacca
     5761 gtaaaaaacc ccgtaaatag cattcgcctg aatttcgcag tgaagccctg aagcttgctg
     5821 aacgcatcgg tgttgctgtc gcagctcgtg tgctcagtct gtatgaatca cagcttgtaa
     5881 gcggctcgtc agaactgtat tgatatttac tgagcggtca gttcaacgta agcgagtgtt
     5941 atgcgccatg gaagcttacc caggaatatg ttatcaggcc tcagcatggc gaagtgttgc
     6001 aacgctggaa gcagaatgac cgcggtcaac aacttgtttt accgcttcaa ttttaaaatc
     6061 ttcgggataa cgcttaccgc tcatgggcac ctctttttaa gccatttaaa atgactctga
     6121 ggtgtctgtt aaacccgtgg cgattcaatc tgga
//
LOCUS       DAZKIF010000204         6149 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZKIF010000204 DAZKIF010000000
VERSION     DAZKIF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.205"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            129..1232
                     /gene="proB"
                     /locus_tag="ACHOTW_004450"
     CDS             129..1232
                     /gene="proB"
                     /locus_tag="ACHOTW_004450"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9846423.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1244..2497
                     /gene="proA"
                     /locus_tag="ACHOTW_004451"
     CDS             1244..2497
                     /gene="proA"
                     /locus_tag="ACHOTW_004451"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9846424.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2612..2687
                     /locus_tag="ACHOTW_004452"
     tRNA            2612..2687
                     /locus_tag="ACHOTW_004452"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2645..2647,aa:Thr,seq:cgt)
     gene            complement(2702..3622)
                     /locus_tag="ACHOTW_004453"
                     /pseudo
     CDS             complement(2702..3622)
                     /locus_tag="ACHOTW_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3567..3812)
                     /locus_tag="ACHOTW_004454"
     CDS             complement(3567..3812)
                     /locus_tag="ACHOTW_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFP9846425.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTNRRIQ
                     MDAQTRRRERRAEKQAQWKADRILASTFRRNATKRHY"
     gene            complement(4052..4441)
                     /locus_tag="ACHOTW_004455"
     CDS             complement(4052..4441)
                     /locus_tag="ACHOTW_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846426.1"
                     /translation="MKKAAILIDAGFFMQRVHATHRKHFAEHELTAQCIMKVIWSMVL
                     SHLNGKRQSQERREPLELYRIYFYDCPPLDIQTRLPLPEPGNKTPGRKNFKLEKSYIL
                     RTELHEELRKTRKTPIVFILKLIGATH"
     gene            complement(4569..5282)
                     /locus_tag="ACHOTW_004456"
     CDS             complement(4569..5282)
                     /locus_tag="ACHOTW_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9846427.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAEKWVSTTKKASGSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            5383..5583
                     /locus_tag="ACHOTW_004457"
     CDS             5383..5583
                     /locus_tag="ACHOTW_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9846428.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            5702..5995
                     /locus_tag="ACHOTW_004458"
     CDS             5702..5995
                     /locus_tag="ACHOTW_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFP9846429.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGGVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            6028..>6149
                     /locus_tag="ACHOTW_004459"
     CDS             6028..>6149
                     /locus_tag="ACHOTW_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9846430.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYS"
BASE COUNT         1505 a         1481 c         1546 g         1617 t
ORIGIN      
        1 gccgctaggc ttgctgatcc cgcgcaacaa aacgccatgc tttgctcgca gatggttggc
       61 aaccgacgac agtcctgcta aaacgttcgt ttgatatcat ttttcctaaa attgaatggc
      121 agagaatcat gagtgacagc cagacgctgg tggtaaaact cggcaccagt gtgctaacag
      181 gcggatcgcg ccgcctgaac cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt
      241 tacatgccgc cgggcatcgg attgttattg tgacgtcggg cgcgatcgcc gccggacgtg
      301 agcacctggg ttacccggaa ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg
      361 tagggcagag tcgactgatt caactgtggg aacagctgtt ttcgatttat ggcattcacg
      421 tcgggcaaat gctgctgact cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc
      481 gcgacaccct gcgtgcgttg ctcgataaca atatcgttcc ggtaatcaat gagaacgatg
      541 ctgtcgctac ggcagagatt aaagtcggcg ataacgacaa cctttctgca ctggcggcga
      601 ttctggcggg tgccgataaa ctgttgttac tgaccgatca aaaaggtttg tataccgctg
      661 acccgcgcag caatccgcag gcagaactga ttaaagatgt ttacggcatt gatgacgcac
      721 tgcgcgcgat tgctggtgac agcgtttcag gcctcggaac tggcggcatg agtaccaaat
      781 tgcaggccgc tgacgtggct tgccgtgcgg gtatcgacac cattattgcc gcgggcagca
      841 agccgggcgt tattggtgat gtgatggaag gcatttccgt cggtacgctg ttccatgccc
      901 aggcgactcc gcttgaaaac cgtaaacgct ggattttcgg tgcgccgcct gcgggtgaaa
      961 tcacggtaga tgaaggggca accgccgcca ttcttgaacg cggcagctcc ctgttgccga
     1021 aaggcattaa aagcgtgact ggcaacttct cgcgtggtga agtcatccgc atttgtaacc
     1081 tcgaaggtcg cgatatcgcc cacggcgtca gtcgttacaa cagcgatgca ttacgccgta
     1141 ttgccggaca ccactcgcaa gaaattgatg caatactggg atatgaatac ggcccggttg
     1201 ccgttcaccg tgatgacatg atcacccgtt aaggagcagg ctgatgctgg aacaaatggg
     1261 cattgccgcg aagcaagcct cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg
     1321 cgtgctggaa aaaatcgccg atgaactgga agcacaaagc gaaatcatcc tcaacgctaa
     1381 cgcccaggat gttgctgacg cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct
     1441 ggcactgacg cccgcacggc tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct
     1501 cgccgatcca gtggggcagg taatcgatgg cggcgtactg gacagcggcc tgcgtcttga
     1561 gcgtcgtcgc gtaccgctgg gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac
     1621 ggttgatgtc gcttcgctgt gcctgaaaac cggtaacgcg gtgatcctgc gtggtggcaa
     1681 agaaacctgt cgcactaacg cggcaacggt ggtggtgatt caggacgccc tgaaatcctg
     1741 tggcttaccg gcgggtgccg tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga
     1801 aatgctgcgt atggataaat acatcgacat gctgatcccg cgcggcgggg ctggtttgca
     1861 taaactgtgc cgcgagcagt cgacgatccc ggtgatcaca ggtggtatag gcgtatgcca
     1921 tatttatgtt gatgaaagtg cagagattgc tgaagccctg aaagtaatcg tcaatgcgaa
     1981 aactcagcgt ccgagcacat gtaatacggt agaaacgttg ctggtgaata aaaacatcgc
     2041 ctatagcttc ctgcccgcat taagcaaaca aatggcggaa agcggcgtga cgttacacgc
     2101 agatgcatct gcgctggcgc agttgcagac aggccctgcg aaggtggtgg cggttaaagc
     2161 cgaagagtat gacgatgagt ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct
     2221 tgacgatgcc atcgcccata ttcgtgaaca cggcacgcaa cactccgatg cgatcctgac
     2281 ccgcgatatg cgcaacgccc agcgttttgt taacgaagtg gattcgtccg ctgtttacgt
     2341 taacgcctct acgcgtttta ccgacggcgg ccagtttgga ctgggtgcgg aagtggcggt
     2401 aagcacacaa aaactccacg cgcgtggccc aatggggctg gaagcactga ccacttacaa
     2461 gtggatcggc attggtgatt acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt
     2521 agccgtttga ttcacaaggc cattgacgca tcgcccggtt agttttaacc ttgtccaccg
     2581 tgattcacgt tcgtgaacat gtcctttcag ggccgatata gctcagttgg tagagcagcg
     2641 cattcgtaat gcgaaggtcg taggttcgac tcctattatc ggcaccattt aaatcaataa
     2701 gttacacatc attagtacct tccttatttt ttgactggga caaatttggg accgatgggt
     2761 tcaggatcga gtctatttgc cgtgcgtgtt cggtaaggtg attaggtgca aggtgagcat
     2821 atcgacgaac catttcgata gactcccagc ctcccatttc ctgtaacact gacaacggga
     2881 ctccggcttg aaccagccaa cttgcccagg tgtgtctcaa gtcgtgaaat ctgaaatcat
     2941 caataccagc ccgtctcagc gccgctttcc aggctgtgtt tgcgtcatac cgcatcttcc
     3001 ttactgttgg cgctttcgtt ccgtctggtt tggtacagct ttccttgtac acaaataccc
     3061 aacggtgatg attaccgatt tgttttttca atacgcgaca tgcagtatca ttcagcgcaa
     3121 cgccaattgc gcggtttgat ttactctctt ccgggtttat ccatgccacc cggcgctgca
     3181 tatctatttg ttgccattca aggttgatga tgttcgagcg tcttaagcct gttgccagtg
     3241 caaattcaac aacagacttt aatggctccg gacattcatc aatcagcctt tgtgcttcat
     3301 ggggctccag ccagcggagc cgtttattct ttggttgagg cactttaata attggtgcct
     3361 tatcgagcat tttccattca cgctctgcgg ctcttagcag ggcctttata aatgaaagat
     3421 gcgtagcctt cgttgcaacg gacgctggtt ttggcgtgta ttctggaaca ggtttccctt
     3481 tttttctgca tgcttctgcc ctgagtttcc agttttcctc atgacgccgg ttcgtcattt
     3541 tctgcattgc tgaataaatt tttgattcag taatgtctct tagttgcatt cctgcgaaat
     3601 gttgaagcca gaatccgatc tgctttccat tgagcctgtt tctctgcgcg acgttcgcgg
     3661 cggcgtgttt gtgcatccat ctggattctc ctgttagtta gctttggttg tgtgtgggag
     3721 tcgtagtcct gaacgaaaac accccgcaat ggcacattgg ctgctaatcc ggattcgcac
     3781 ttccggccaa tgcttcgttt cgtatcacac acaccaaagc cttctgcttt gaatgctgcc
     3841 cttcttcagg gcttaatttt taagagcatc accttcatgg tggttagtgc gtcctgctga
     3901 tgtgcttagt atcaccgcta gtggtatttg tgtcaacacc gccagagata atttatcacc
     3961 gcagatggtt atctgtatgt tttttatata gatttatttt ttgcagggtt gtgtggcttg
     4021 ggaggtgatc gagagatcag aattgcgttg tttagtgagt tgcacctatt aattttaaaa
     4081 taaatacaat tggtgttttt cgagtttttc ttaactcttc atgcagctcc gttctcagaa
     4141 tatatgattt ttcgagtttg aaattcttgc gaccaggcgt cttattgcca ggctcaggaa
     4201 gtggaaggcg tgtttgaatg tcgagtggtg gacagtcata gaagtaaatt ctataaagct
     4261 caagcggttc cctacgttct tgtgattgac gttttccatt aagatgggaa agaaccattg
     4321 accatattac tttcattatg cattgcgcag tcagttcatg ctcggcgaag tgtttacgat
     4381 gcgtagcatg aacacgctgc atgaaaaaac ccgcatcaat taaaattgct gctttcttca
     4441 taagagtccc aaaaaaatag cccagagccg ttatgcagat attaacaatt gtctgcgaac
     4501 ggggctgggc ttggtttaat taatctatgc caagttgact gtgccgtcaa cataaaactt
     4561 acttcctatc acccaaacgt ctcttcaggc cactgactag cgataacttt ccccacaacg
     4621 gaacaactct cattgcatgg gatcattggg tattgtgggt ttagtggctg tagaaacacc
     4681 tgaccgctat ccctgatcag tttcttgaag gtaaactcat caccaccaag tctggctata
     4741 cagaaatcgc ctggctcaac agcctgctca gggtcaacca gaattaacat cccgtcagga
     4801 aagctgggct tggatcctgt tggtgcggtc atggaattac cttcaacctc aagccagaat
     4861 gcagagccac tggctttttt ggttgtgctt acccatttct ccgcatcgcc tttggtaaag
     4921 gttctaagct caggcgagaa catcccggcc tgaacatgag aaaaaacagg gtactcatac
     4981 tcacttctaa gtgacggctg catactaacc gcttcataca tctcgtagat ttctctggcg
     5041 attgaagggc taaattcttc aacgctaacg ttgagaattt ttgcaagcaa tgcggcgtta
     5101 taagcattta atgcattgat gccattaaat aaagcaccaa cgcctgactg ccccatcccc
     5161 atcttgtctg cgacagattc ctgggataag ccaagttcat ttttcttttt ttcataaata
     5221 gctttaaggc gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt ttttgcgctc
     5281 atacgttaaa tctatcaccg caagggataa atatctaaca ccgcgcgtgt tgactacttt
     5341 acctctggcg gtgataatgg ttgcatgtac taaggaggtt gtatggaaca acgcataacc
     5401 ctgaaagatt atgcaatgcg ctttgggcaa accaagacag ctaaagatct cggcgtatat
     5461 caaagcgcga tcaacaaggc cattcatgca ggccgaaaga tttttttaac tataaacgct
     5521 gatggaagcg tttatgcgga agaggtaaag cccttcccga gtaacaaaaa aacaacagca
     5581 taaataaccc cgctcttaca cattccagcc ctgaaaaagg gcatccaatt aaaccacacc
     5641 tatggtgtat gcatttattt gcatacattc aatcaattgt tatctaagga aatacttaca
     5701 tatggttcgt gcaaacaaac gcaacgaggc tctacgaatc gagagtgcgt tgcttaacaa
     5761 aatcgcaatg cttggaactg agaagacagc ggaagctgtg ggagttgata agtcgcagat
     5821 cagcaggtgg aagagagact ggattccaaa gttctcaatg ctgcttgctg ttcttgaatg
     5881 ggggggcgtt gacgacgaca tggctcgatt ggcgcgacaa gttgctgcga ttctcaccaa
     5941 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     6001 ttactggatc tatcaacagg agtcattatg acaaatacag caaaaatact caacttcggc
     6061 agaggtaact ttgccggaca ggagcgtaat gtggcagatc tcgatgatgg ttacgccaga
     6121 ctatcaaata tgctgcttga ggcttattc
//
LOCUS       DAZKIF010000205         6116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZKIF010000205 DAZKIF010000000
VERSION     DAZKIF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.206"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(108..710)
                     /gene="fimB"
                     /locus_tag="ACHOTW_004460"
     CDS             complement(108..710)
                     /gene="fimB"
                     /locus_tag="ACHOTW_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP9846431.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2166..2882
                     /gene="nanC"
                     /locus_tag="ACHOTW_004461"
     CDS             2166..2882
                     /gene="nanC"
                     /locus_tag="ACHOTW_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP9846432.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2902..4008
                     /gene="nanM"
                     /locus_tag="ACHOTW_004462"
     CDS             2902..4008
                     /gene="nanM"
                     /locus_tag="ACHOTW_004462"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP9846433.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4073..5053
                     /gene="nanS"
                     /locus_tag="ACHOTW_004463"
     CDS             4073..5053
                     /gene="nanS"
                     /locus_tag="ACHOTW_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9846434.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            5176..5871
                     /locus_tag="ACHOTW_004464"
                     /pseudo
     CDS             5176..5871
                     /locus_tag="ACHOTW_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1808 a         1340 c         1314 g         1654 t
ORIGIN      
        1 ttaagaatca tacttacaat aacatcaatt aataaatggt taatcatttt tgatatatcg
       61 taagaataat gtagttttta acaccatccc tggtatctca actatctcta taaaacagcg
      121 tgacgctgtc gtcctctggc tctatcccag atgccgtaaa aacgccctgc attgctggca
      181 gtataccaga cagtatgacg aatattgcga tgcccaagat aatcctggat aagtcgcgta
      241 tctattccca tattcgccaa agcaaaacca cacgaatggc gtaacatgtg cggatgaatc
      301 tccagtgaca acccggcatt accaccggaa gtcgagataa tatggtaaaa ttgttgccga
      361 gaaagcggat tccccttacg cgataaaaat acccactcgc tctcagcatg cggataagaa
      421 gtacggatac tcaaccagtt ttttaaagcc tgaacttctt tgttcaatag cgggtgcgtt
      481 gttgaaaagc ctttttttaa tcgatggata tatatacact ttgccttaag atcaatatcc
      541 gaaatcctca atcgacaaat ttcactcgcc cggaaaccat gaataaaaca aagcaaagtc
      601 agacaataat tacgtgctgc atgaggcccg gtatttgctg ctttaaggag tgattcgatt
      661 tcactatggg tcaggaagtt cctttttttg ttatcagcct tattcttcat cgtttttccc
      721 ttataattac agacgcgcac tagctgtgct aggttagcaa taatccaaca tttttatcct
      781 gattatgttt ataaaagcga acgtttgctt aataactaac gtaagtggac cagttcttct
      841 gagtgaactt aaatggagta gcaacagtta attataataa cgttgccata caaacgctat
      901 gctcttgcat gctatgtacc tttatatatt tatcaatcgg atacgaatct aataataacc
      961 ctcatcatta atgcaactaa tcttatctat atatcatgtg atatgttctg taacaagtaa
     1021 aatcaacata aaaaattcag caaaatttaa tattcgttca actagccgtc tcacatatga
     1081 cattattagt caactccatt tgccacaaaa tggctatttc atggaacaca gtaatttctt
     1141 agtggctatt atcaagctaa ttcttaggtc cctagcgatt attcctggct gattcgtcag
     1201 cttacgccat gagattgtta cattcctttt tatcctggag atatggggga acgcaggaga
     1261 tgattttttc atctacattc gttggtaaat atagctttgg tcactacgac ttaaaccttc
     1321 ctcaatcaca gccattcgcc gggcgatacg gcaaacaaac taagtgaaac atcatcctga
     1381 aaacaccaac atcaataagc ctctccagat cgacttcaga agtgaccagt tacaagccac
     1441 aaaccaggaa acatatttca tgatgagaat tatgctcagc ctgatggcgg gtaaacactg
     1501 atttacacca agctcactga gttagcttgc aaagcttccc ttgtttagac tctaaataaa
     1561 ttatttataa aacaaatagt taaacgaaga tctttagttt ttgaagacca ccgcaagtgt
     1621 tcgtctggct tcacatggca tcttcttctt agaaaaagat cgacatattt tgtgacacga
     1681 attgcaaatc tggttttgtt gtatggattg cgtgattttc gatctggtat aacaggtata
     1741 aaggtgcacc acgatagaca atgagacagg gcatcttgca atctatggca aacatcactt
     1801 cagttctttc tcatcgggtg atgaaaacgc acttcagcct gaaaggaata tgaaaatgag
     1861 atcaacagac actctatttt atgactctgg gtaaaatgaa ttgagtaagt gatatagctt
     1921 acgaacattc aaatcaatta aacatcagaa tagattttat actcaggtat ttaatctggc
     1981 tctctgttta tttaaataat gtggaaagag atttttcaca ggagacatta tacaaaaaat
     2041 ataaaataca gctaccggtt gccaaagaca ctataagcct ggcaaaaaaa tattacacaa
     2101 cataaatgct aattgtttat gcgggctttg tattgctttc tgtatcctac aaatgagtga
     2161 aatttatgaa aaaggctaaa atactttctg gcgtattatt actgtgcttt tcgtcaccat
     2221 taatttctca ggctgcgaca ctggacgtac gtggtggata tcgtagtgga agccacgcct
     2281 atgagactcg actcaaagtc agtgagggat ggcaaaatgg atggtgggca agcatggaaa
     2341 gtaatacctg gaataccatt catgataata aaaaggaaaa tgccgcactc aatgatgttc
     2401 aggttgaagt taattacgcg attaaacttg atgatcaatg gacggtgcgc ccgggaatgt
     2461 taacgcattt tagcagcaac ggcacacgct acggacccta cgtaaaactg tcctgggacg
     2521 cgacaaaaga tcttaatttt ggcattcgct atcgttacga ctggaaagct taccgacaac
     2581 aagacttatc cggtgatatg tctcgtgata acgttcatcg ttgggatgga tatgtcactt
     2641 accatattaa tagtgatttc accttcgcat ggcaaacgac gctatacagc aaacagaacg
     2701 attatcgcta tgcaaaccat aagaaatggg cgacggaaaa tgcatttgtt ctacaatacc
     2761 atatgacgcc cgatattacg ccatacatag aatatgacta cctggaccgt cagggtgttt
     2821 acaacggcag agataattta tcggaaaaca gttatcgcat tggtgtgtca tttaaactgt
     2881 agtagacagg agacagtcac aatgaataaa acaataacgg cgcttgctat cctaatggct
     2941 tcatttgccg caaacgcgtc tgtattaccg gaaactcctg taccatttaa aagtggtacc
     3001 ggagcaattg ataacgacac tgtctacatt ggtttaggta gcgcaggtac ggcatggtac
     3061 aagctggaga cacaggccaa agataaaaaa tggacagcgt tagctgcatt ccctggtgga
     3121 ccaagagatc aagcaacctc ggcatttatt gatggcaatc tgtatgtgtt tggcggcatt
     3181 ggcaaaaaca gcgagggctt gactcaggta tttaatgacg tacacaaata caaccccaaa
     3241 accaatagct gggttaaatt gatatcgcac gcgccgatgg gcatggcggg ccatgtgact
     3301 tttgtacaca acggcaaggc ttatgttact ggcggtgtta accagaatat cttcaatggc
     3361 tattttgaag atctcaacga agctggaaaa gattcaaccg ctgtagataa aatcaatgca
     3421 cactattttg acaaaaaagc agaagattat ttctttaata agtttctgtt gtcttttgat
     3481 ccctctacac agcaatggag ttacgctggc gaatctccct ggtacgggac ggctggtgcg
     3541 gcggttgtga ataaaggtga taaaacctgg cttattaatg gcgaagccaa acccggattg
     3601 cgaacggatg ccgtatttga acttgatttc accggtaata atttaaaatg gaataggctt
     3661 gctcccgtct catcaccaga tggcgtcgct ggcggttttg cggggataag caatgattct
     3721 cttatatttg ccggaggggc cggattcaaa ggttcacgag aaaattacca aaacggtaag
     3781 aactatgcgc atgaaggcct gaaaaaatca tatagcactg atattcatct ttggcataac
     3841 gggaaatggg ataaatcggg tgaattatcg caaggtcggg cctacggagt atcattgccc
     3901 tggaataata gtctattgat tattggcggt gaaactgcag gcggcaaagc ggtgacggat
     3961 tcagttttga tctctgtgaa ggataataaa gtcacagtac aaaactaacg cttcagggcc
     4021 ccggtaaagg ggccctgcta tcaatttgtc atttcaatta cgcgaaattt atatgaacgc
     4081 aataatatcg cctgattatt actatgttct tactgttgct ggtcagtcta atgccatggc
     4141 gtatggcgaa ggactgccat taccggacag ggaagatgcg cctcatccca gaattaaaca
     4201 attagcgaga tttgcgcata cgcatcccgg aggcccgtca tgtcacttta acgacattat
     4261 tccactgact cactgcccac acgatgttca ggatatgcag agttatcacc atcctctggc
     4321 aacgaatcat caaacacagt acggcaccgt tggccaggca ctgcatattg cacggaaatt
     4381 actgcccttc attcctgata atgcagggat tctcatcgtt ccgtgttgcc gtggcggatc
     4441 ggcttttacc gcaggcagcg aggggacata ttcagaacgg cacggagcca gccatgatgc
     4501 ttgtcgttgg ggaacggata ctccgctata ccaggattta gtcagcagaa cgcgagccgc
     4561 actggtaaaa aatccgcaga acaaattcct cggcgtatgc tggatgcaag gcgaatttga
     4621 cttaatgacc agtgactacg cgtcacatcc tcaacacttt aatcatatgg ttgaagcctt
     4681 tcgtagggat ctaaaacaat accattctca gcttaataat attactgacg cgccgtggtt
     4741 ttgcggcgat accacctggt actggaaaga aaatttccct catgcgtatg aagctattta
     4801 tggcaattat caaaataata ttttagctaa tattattttc gtcgacttcc agcaacaagg
     4861 tgcaagaggg ctgacgaacg cgcctgatga agatccggac gatttaagca cgggatatta
     4921 cggttcagcg taccggtcac cggagaactg gacgacggca ctgcgaagca gtcatttcag
     4981 ctcagcagcc cgtcggggga ttatttctga caggtttgta gaagcaattt tgcagttttg
     5041 gcgcgaaagg tgagcgttca ttaatttgat ttattcgcat ccatcagcgc ttttaactcc
     5101 tccggcgtgc ggcactataa cgacttccac aacaaggagc cattttctta taccactttc
     5161 agtgaccatc cgcttatgcc accaccctct acgcaactgt ttgcagagca ccttcccacc
     5221 gagtggatac agcacttcct gacgctttct gctcatgcga ccgttcgtcg tcttaacctg
     5281 agcgtggatg gcgaagcggg gatgaacctg ctggcgcccg cagcgctgtc acccaggcgc
     5341 gtcagcgcgt gggggccgcc ccggtggaat ggctcttccg ccagaccaca cagacatggg
     5401 gcgcggaacg ttacctgaag gatgactggc acggcctgca actttttgcc attgatggcg
     5461 cacagttcag gacacctgat gaacccgagc tgcgtgaata ttatggctct gccaacacat
     5521 ccactgagcg gcagagcgcc taccccgtca tgcgtctggt ggcgttaatg aatctgggaa
     5581 tcacattcta ctgaatgcca tgaccgcgcc taccggcgga gcgatacggc gtgaggcaac
     5641 aaaagcggcg tcagagatca gctttaagtt cgcgttccag tttatcgtga cagaaatgat
     5701 agtgctggga aacacggcgt caccggggac tatcccgaaa cgactggagc atctgcgggg
     5761 gctctggaag tggtgttcat aacaaaacgc ccccgaccat caagggcgag ggcggttaag
     5821 atatcaaaaa cccgttatcc tgtgaaacac agtgccgccc cgcttaagtg aacggcattg
     5881 tgtttctgag ggaccttaat tttccctgat tctctgacgt tccttaccat tgatatttcc
     5941 ttcattcctc accagagaaa taaaaaatca ctatactcat caaaatatga gattaccttt
     6001 attcaggtga tcaccttact attttttacg gaaaataaat tatgaataac aagaaatcac
     6061 tgacaaacca cctgtaaaat gtcaatcatt ttagttcaat tgcctttgtc atgatt
//
LOCUS       DAZKIF010000206         6077 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZKIF010000206 DAZKIF010000000
VERSION     DAZKIF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.207"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(67..600)
                     /locus_tag="ACHOTW_004465"
                     /pseudo
     CDS             complement(67..600)
                     /locus_tag="ACHOTW_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(605..769)
                     /locus_tag="ACHOTW_004466"
     CDS             complement(605..769)
                     /locus_tag="ACHOTW_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846435.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(766..1446)
                     /locus_tag="ACHOTW_004467"
     CDS             complement(766..1446)
                     /locus_tag="ACHOTW_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9846436.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            complement(1534..1995)
                     /locus_tag="ACHOTW_004468"
     CDS             complement(1534..1995)
                     /locus_tag="ACHOTW_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9846437.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1988..3028)
                     /locus_tag="ACHOTW_004469"
     CDS             complement(1988..3028)
                     /locus_tag="ACHOTW_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9846438.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAAPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(3000..3551)
                     /locus_tag="ACHOTW_004470"
     CDS             complement(3000..3551)
                     /locus_tag="ACHOTW_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9846439.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            complement(3535..3762)
                     /locus_tag="ACHOTW_004471"
     CDS             complement(3535..3762)
                     /locus_tag="ACHOTW_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9846440.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3839..4246
                     /locus_tag="ACHOTW_004472"
     CDS             3839..4246
                     /locus_tag="ACHOTW_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9846441.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4510..4809
                     /locus_tag="ACHOTW_004473"
     CDS             4510..4809
                     /locus_tag="ACHOTW_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846442.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4882..5100
                     /gene="ydfC"
                     /locus_tag="ACHOTW_004474"
     CDS             4882..5100
                     /gene="ydfC"
                     /locus_tag="ACHOTW_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP9846443.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5123..5530
                     /locus_tag="ACHOTW_004475"
     CDS             5123..5530
                     /locus_tag="ACHOTW_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846444.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5508..5741)
                     /locus_tag="ACHOTW_004476"
     CDS             complement(5508..5741)
                     /locus_tag="ACHOTW_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846445.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT         1514 a         1576 c         1333 g         1654 t
ORIGIN      
        1 tgcatagctt gttatttaaa gtcaacaaaa taaggaaaat tatacgcata ttgaagagta
       61 taaaccttac atgttgatta catttttgta atcaacatcc tgtttggaat agccagcctt
      121 taatggataa ctatttctga caatgcaatg agtataatca agtccatctt ccactgagaa
      181 ttagaggcgg catgcttttt ccggctcttg ccggatatcc gtaattgtcc ataatctgca
      241 gatttatacc ttctgcatga cctgtcagcg aaaatttgtc cggtgtttct acgggaatga
      301 catcaaaagt tacacgcact cgcgttaccg tgtagaccta ctttcctgca cttgcaagat
      361 cacagtggtg taaccgtaac aggaatttat tctctggacc ggcagtaaat ccctgagtgg
      421 cgtggttccc atatcaattt cccgccaggc agcctccatt gccagcgtac aggctggagc
      481 catgacctgc cctttaaatc tggcccgacc atcccaccgg acgtgttctt ctcccctgaa
      541 cttaggtaca gtcatctcca gtggcacaaa agtgtcagcg ccataatttt tgaccgttat
      601 cgcgctacgg atattttgtt gactggtgaa aatcaccccg cagaatcagg cttattccct
      661 taacctgggc tttcatcctg accgccgcct cactacgacc aatcagactg ccgatgcatt
      721 ttaccttcat tgttagtatc atgatttcag gcctgcacca tccgctcatt gcccggactt
      781 ccgacaaatc ccggcaacca tatcccggtg cttgttcagc tcccgcagcg cggcgcagac
      841 tcgctcccac ttctggacat gattcttcgc cctacgcagt tcgcgatttg ccatatgcag
      901 cgatggcaaa atcaaatcat cttctcgcgt tgcagtaaac gatggcagcg actgcacaat
      961 gtccgccaca gtttctgttt taatatcttc ctgtgttgca tctttctgta ccggtaacgc
     1021 aacaccggct ggctgaggaa aggctttacc atcggtttcc gctaccgatt caactttcgg
     1081 ctctgctggt aaattatcac ctggtatgca gtaacgaatt ttaccgttct ggtttacgcg
     1141 aatcagacga cctttgctga ttgccattgc cagcgttgaa gcaactttgc gggatgttgt
     1201 accgaacagc gtagccagtt catccgccgt ttgtggtccg cgttgttcaa tcgtcgcggt
     1261 taaatcgcac tctgagattt tcgcttctgt tgccgtggtg gtttcttctg gcgctggctg
     1321 ttcctgctga acgttgttat cagccacacg ccaggtgtat acgcttttat caacgaagcc
     1381 agcctttttc agttcccaca gctcgttcag tacttcttca cgactgatat caagtcgcgc
     1441 agccagctat accgacgtgg cttttcccat cgctttcagt gcgtcaaaaa cagtctccat
     1501 aaatttcctc ccggtaaaaa atcacttctc aactcaaaca aaaccagccg ctttccggcg
     1561 ttcatattcc tgtttcagca actcaattgg cgttggcccc gacgggcgtt tgggggccgc
     1621 cagttgtcgc cggactggcg gaacgctcag gccgttacta acatgctttg cccatttcgt
     1681 cagttgccgt tctgcaagcc gttttaattc cccttcggtc atctggcgct caatcccctt
     1741 tgaacgcatc tcgaggcaaa tgtgatacag cacaggctga gaccacgggt acttatcact
     1801 tccgtcgtat cgccaggact cattgcgcca gcggcggtac tcctccatca cagcatccac
     1861 cgtcagaccg aatggattgg ctccgctttt cgaaatcagt gccacaaact cagccaggtc
     1921 cggaggccat gtttcacccg cccggcagcg gtccatgcac tggcggcaga cctgccggat
     1981 ttgctgctca gtcatcgcgc caatctgtgc aatccagagc ttcgaaggtg cggccccgtt
     2041 cttctggatc cagcggttcg aataaaccac ccccatgagt tcccacagct tccaggccgt
     2101 ttccgtcgct gataaatccg ttttcacgtt cccactgctc acgtgctgcc cgaatttcct
     2161 gaactgcccg tgatgcggtg ccacctggtg ctgctgcatg gtttacccct ttgctgactg
     2221 gtttaacctg cgccctgacg tgatttacgt gacgggcgaa tttctgctcc cactgaatct
     2281 gcgtaaacac tttcccctcc gctgcccagt agtcccggaa ggcggcaagt tcagcaggtg
     2341 taaattctgt ctccggcaaa gccatccccc acaacgcagc ccgtcgtcga aaatcccgtg
     2401 acggatacca gctatcgctc atcgggaatt ttccgatggg ttcgctcagg ccatacagga
     2461 atacaggggc ggctgcctgt aacgacaaaa cttcctgctc actggtcgga gcactctcgc
     2521 gtgcgttatg tgtggggttt agatctttgg gttcctttgg gttccgtgat ccgtttttgg
     2581 gtgtctttga tggaaaattt gggtgtcttt ggttattttc catgcagcta agagttctgt
     2641 ttttgggtct gttttgtgct gaaacataac cattttcggt actgttttta ttaacagcac
     2701 caattttacc cacctttaaa gactcccgtt tttgggtgta ttcaggctcg gcaacacttt
     2761 cttctacacc gataagtcgg tacaccacaa tttgctttgt tctgcctttt ctctcaccgg
     2821 tatcaacaat taacccaatc tccatcaggt gtcgtaagct gtcctgcaca gtctttttgt
     2881 tcagttccgt tacttctgcc agtgcagata cagacgggta tgcacacaaa tcggcaccgc
     2941 acatatcagc aagccaggtc aatactgact tactggatga actgccggtt ttcacctttt
     3001 tagcccatcg tagtgcatcg atactcatac gcccccctgg cagacatttg tttatctgca
     3061 aagtaatatt ggtattgctg acgatacgcg tgcttgaaag caatagcttt ttctataagc
     3121 tcgtcagtct cacgttccac aacaactgga tccgcaaaaa gcagcccgga ctccaccaca
     3181 tcgccatatt ctttgtttaa cccggcgatc atgtacgtaa tgctttttcc gtcagtaatt
     3241 tcacaataca atctgaaatc gctgatccgg atagcctcca taattgccgg aatcagcgcc
     3301 gtgaatttgt cacgcttatc tctggtgtcg atagctttcc agcgttcgaa tatcttcacc
     3361 cggttaacgc ccagcgcccg ttgatcaacc tcgccatcat taaacgtgac gcgttgaaca
     3421 tcgatgttcg ggcgttcttt cagagcccag aatgcttccg tgattaatat cgtcgcctgc
     3481 tcctgtgtca ttcctggtcg gcatacccag gtatccagag cctcacaaac ctgttcagtg
     3541 gtgattttca ttgttcaacc gccccgcccg ctttgcctta cgatattcgt cataaacttt
     3601 ggggtcgtac tgaagttccc cgccggatgc ctcttgcagg cgcatcgcgc gaccttcagg
     3661 aaccagttcc ccccatgcag caacgcttgc cagcctaact cctgcgacat tggcaagctt
     3721 tgttttgctg ccaaaaaacg ctatagcatc aattttcaac atatcgaaac ccttagattt
     3781 tcctaaggaa actagatcgt agagaaacct aagtcaagaa aaattagaat tacctaatat
     3841 gaaaaacgaa accttcggtg ctcgcctctt atacaggcga aaaaaattaa aactgtctca
     3901 ggccgcatta ggtaagctgg tcaaagtcgc tcacgtaaca atttctcaat gggaaagaga
     3961 tgaaacacag ccagcgggga agagattatt cgcactgagc caggcgcttc agtgctcgcc
     4021 gacttggctt ctttttggag atgaagataa gcaaccaggc gaaccgatcc cagataatca
     4081 gccagtcaat ctgacagaag atcaaaaaga gttgcttcaa ctgttcgacg cactgcctga
     4141 gtcagagcaa aaggctctgt tgtcagagat gcgtgctcga gttgagaatt tcaacaaact
     4201 ttttgaagaa ctactcaaag ctcgcaaaag aagcgcaaat aaataacccc cctttttttc
     4261 gcaactctct gtaataaaaa gcacaaactt tcaaatactt gtgtttttta catcaagagg
     4321 cttaggtttt tctacacaaa aagcttgacc ataactctta ggctattcta aattctactc
     4381 atcaagacac cgcacggtgt tctcagcaaa cagttccgct acccggcgtt aaggggaagc
     4441 agaggatttc tcagtgggcg agtcaaacat cagaatggaa ggcgtccagg gatcagcaaa
     4501 gaaacagcga tggcgcttta tattgatatc agcgccattg ccggacaggt aagagttatc
     4561 agagcggtaa ctaagcggta tgcgccttta cttcagaaag tctctggtga gtgcaccgaa
     4621 gatattgtca acgatttcgt catcgaactg cgaggactca tcttcagtta caaggtgacc
     4681 acaatttttg cagatggctc ccgcgaaact gtcagagccc tgcggcttaa aggatgtgtc
     4741 aaagacttag ccaccacatt ctgggcaaga aaacttgatt gtattcataa ccaatttcct
     4801 ctcgagtaac agacccctca gaggatacca cctcgcctga cgtggttaaa aagcaggcaa
     4861 cgctaaccac aaggagccga catgcagaaa cgagaccccg tcatcatcgc gccagactat
     4921 accgatgatg aactttatga gtggatgcac cagaaaatta aggcggtgca ggacctgaaa
     4981 tgggccaatg aagccagggc taagcaggct gaaaatctgt ccgctctgga gcaggatatc
     5041 accaatctgg aaaaagcagc ggcattaagc attgccagaa tgattacata cccacgttaa
     5101 tagctaacca acgaggctaa taatggaatt taaagattta ccaacgccat tacaggaaat
     5161 ggcatcgaat atagttcgtt cacaactggc tactcttgac cttagtaccg cagaaaaaga
     5221 aaccatcgat aatatggttc gtaatgtgcg caatgctttt tctgggctat atggttctga
     5281 taatcaaaag caggaaagcg atgttaataa acgggtaatt tctgtttgcg tgaatggcca
     5341 tgttctttca tcaatcaaaa cagaaacggc gacagtcttc gattgccttt gcattgtaca
     5401 gagccttgtt gatgccctgt ttcgttcagt gaatttagaa aatgatgcaa atctgcgagg
     5461 gcgcataata gcacatccat atgcacatac tttaggctct gtggatatca aagatcccac
     5521 aaatctttaa tgaaatagtt aacgcgaatt gtacttgctc tttcggttgc tttcagaata
     5581 cgcgttgaaa ctgctggcgg taatttggta ttccatttat taaaatcatg cccgggaaag
     5641 tactcttcga aaatactttt aactgcagac tcgcctattg aaatgctgct taccatgcga
     5701 ttttgataaa ggcatttagc aataagagtt gattttaaca ttcaccctcc tgagggttgg
     5761 taattaagga gttctccacg ggtgaagtgg agtgcgtgcg ccggacacgg gtgaacatcc
     5821 ggcactgaca gtttactgaa aggatatttc tctgaaaagt cagagcataa cgcgaaatgg
     5881 cgcagcggta agtatggcgg ggttattcct tccccgttga ggacaccggg ttgtcaggtt
     5941 gaccatacgc ttaagtgaca accccgctgc aacgccctct gttatcaatt ttctggtgac
     6001 gtttggcggc atctgtttgc ccatgaactg atgtccgccc tttttaaagt gaattttgtg
     6061 atgcagtgaa tgcggct
//
LOCUS       DAZKIF010000207         6069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZKIF010000207 DAZKIF010000000
VERSION     DAZKIF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.208"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            252..1355
                     /gene="ompC"
                     /locus_tag="ACHOTW_004477"
     CDS             252..1355
                     /gene="ompC"
                     /locus_tag="ACHOTW_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9846446.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1467..2522
                     /gene="apbE"
                     /locus_tag="ACHOTW_004478"
     CDS             1467..2522
                     /gene="apbE"
                     /locus_tag="ACHOTW_004478"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP9846447.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2596..3660
                     /gene="ada"
                     /locus_tag="ACHOTW_004479"
     CDS             2596..3660
                     /gene="ada"
                     /locus_tag="ACHOTW_004479"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP9846448.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3660..4310
                     /gene="alkB"
                     /locus_tag="ACHOTW_004480"
     CDS             3660..4310
                     /gene="alkB"
                     /locus_tag="ACHOTW_004480"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP9846449.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4386..6029
                     /gene="yojI"
                     /locus_tag="ACHOTW_004481"
     CDS             4386..6029
                     /gene="yojI"
                     /locus_tag="ACHOTW_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP9846450.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLSLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1441 a         1529 c         1642 g         1457 t
ORIGIN      
        1 cttgaattat tattgcttga tgttaggtgc ttatttcgcc attccgcaat aatcttaaaa
       61 agttccctcg aatttacatt ttgaaacatc tatagcgata aatgaaacat cttaaaagtt
      121 ttagtatcat attcgtgttg gattattctg catttttggg gagaatggac ttgccgactg
      181 attaatgagg gttaatcagt atgcagtggc ataaaaaagc aaataaaggc atataacaga
      241 gggttaataa catgaaagtt aaagtactgt ccctcctggt cccagctctg ctggtagcag
      301 gcgcagcaaa cgctgctgaa gtttacaaca aagacggcaa caaattagat ctgtacggta
      361 aagtagacgg cctgcactat ttctctgacg acaagtctgt agatggcgac cagacctaca
      421 tgcgtcttgg cttcaaaggt gaaactcagg ttactgacca gctgaccggt tacggccagt
      481 gggaatatca gatccagggc aacagcgctg aaaacgaaaa caactcctgg acccgtgtgg
      541 cattcgcagg tctgaaattc caggatgtag gttctttcga ctacggtcgt aactacggcg
      601 tagtttacga cgtaacttcc tggaccgacg ttctgccaga attcggtggt gacacctacg
      661 gttctgacaa cttcatgcag cagcgtggta acggtttcgc gacctaccgt aacactgact
      721 tcttcggtct ggttgacggc ctgaactttg ctgttcagta ccagggcaaa aacggtagcg
      781 taagcggcga aggcatgact aacaacggtc gtgaagcact gcgtcagaac ggcgacggcg
      841 ttggcggatc tatcacttat gattacgaag gcttcggtat cggtgctgca gtttccagct
      901 ccaaacgtac tgatgatcag aacagcccgc tgtacatcgg taacggcgac cgtgctgaaa
      961 cctacactgg tggtctgaaa tacgacgcta acaacatcta tctggctgct cagtacaccc
     1021 agacctacaa cgcaactcgc gtaggttccc tgggttgggc gaacaaagca cagaacttcg
     1081 aagctgttgc tcagtaccag ttcgacttcg gtctgcgtcc gtccctggct tacctgcagt
     1141 ctaaaggtaa aaacctgggt gtcatcaatg gtcgtaacta cgacgacgaa gatatcctga
     1201 aatatgttga tgttggcgcg acctactact tcaacaaaaa catgtccacc tatgttgact
     1261 acaaaatcaa cctgctggac gacaaccagt tcactcgtga cgctggcatc aacactgata
     1321 acatcgtagc tctgggtctg gtttaccagt tctaatctcg attgatatcg aacaaagggc
     1381 ctgcgggccc ttttttcatt gttttcagcg tacaaactca gttttttggt gtactcttgc
     1441 gaccgttcgc atgaggataa tcacgtatgg aaataagctt tacccgcgtg gcactgctgg
     1501 ctgccgcgct cttctttgtt ggttgcgatc aaaaaccaca acccgccaaa acccacgcta
     1561 ctgaagttac cgttcttgaa ggcaaaacta tgggtacctt ctggcgtgcc agcatcccgg
     1621 gcattgacgc caaacgcagc gccgaactta aagaaaagat tcagacccag ctggacgccg
     1681 acgatcagct gctttcgacc tataaaaaag attccgcgct gatgcgcttt aacgactcgc
     1741 aaagtttatc gccgtggccg gtaagtgaag cgatggccga tatcgtcacc acctcgctac
     1801 gcattggcgc gaagaccgat ggcgcgatgg atataaccgt cgggccgctg gtgaatctgt
     1861 ggggtttcgg tccggaacaa cagccggttc aaattccgag ccaggaacag atcgacgcga
     1921 tgaaagccaa aactggttta cagcacctta cggtcattaa tcagtcgcat cagcaatatc
     1981 tgcaaaaaga cctgccggat ttatatgtcg atctctccac cgtcggcgaa ggttatgcgg
     2041 cggatcacct ggcacgcttg atggagcagg aagggatttc ccgctatctg gtgtcggtgg
     2101 gcggcgcgct gaacagccgt ggtatgaacg gtgaaggcct gccgtggcgg gtagcgattc
     2161 aaaaaccaac cgataaagaa aacgcggttc aggccgtggt ggatatcaac ggtcatggga
     2221 ttagcacctc tggcagctac cgtaactatt acgaactgga cggcaaacgt ctttcccatg
     2281 ttatcgatcc gcaaaccggg cgtcccatcg aacacaatct ggtatccgtg acggtgattg
     2341 caccgacggc gctggaagcc gacgcctggg atacaggttt gatggtactc gggccggaga
     2401 aagccaaaga agttgttcgc cgggaagggc tggcggttta tatgatcacc aaagaaggcg
     2461 atagctttaa aacctggatg tcaccacagt ttaaaagctt ccttgtcagc gaaaaaaatt
     2521 aaagcgcaag attgttggtt tttgcgtgat ggtggccggg cagactaaag gctatcctta
     2581 accagggagc tgattatgaa aaacgccaca tgcttaactg acgatcaacg ctggcaatct
     2641 gtcttagccc gcgacccgaa tgccgacggc gaattcgttt tcgccgtgcg caccacaggc
     2701 atcttttgcc gtccgtcttg ccgcgccaga catgctttgc gggaaaacgt ctccttctac
     2761 gcaaatgcca gcgaggcact cgccgccgga tttcgcccct gcaaacgctg ccaacctgat
     2821 aaagccaatg cccagcaaca tcggttggat aaaatcaccc acgcgtgtcg actgctggaa
     2881 caggaaacgc ctgtaacgtt ggaagcctta gccgaccagg tggcgatgag tccatttcat
     2941 ctacatcggt tgtttaaagc gactaccgga atgacgccta aagcctggca acaggcctgg
     3001 cgcgctcgcc gtttgcgcga atcgctggcg aaaggggaga gcgtgacgac gtctattctt
     3061 aacgccggat tccccgacag cagcagttac taccacaaag ccgatgaaac gctgggcatg
     3121 acggctaaac aattccgtca cggtggcgaa aatctggcgg tgcgttacgc gctggctgat
     3181 tgtgagctgg gtcgttgcct ggtggcagaa agcgagcggg ggatttgcgc gatattgctg
     3241 ggcgatgatg acgcgacatt aatcagcgag ttgcagcaga tgtttcccgc tgccgacaac
     3301 gcgcctgccg atctgacgtt tcagcaacat gtgcgtgaag tgatcgccag cctcaatcaa
     3361 cgcgatactc cgctgacgtt accgctggat attcgcggca ctgcttttca gcaacaagtc
     3421 tggcaggcac tgcgcacgat accttgcggt gaaaccgtca gttatcagca actggctaac
     3481 gccatcggca aaccgaaagc ggtacgggcc gttgccagcg cctgtgccgc caacaagctg
     3541 gctatcgtaa ttccctgtca tcgagtggtc cgtggtgatg gcacactttc cggttaccgc
     3601 tggggcgtgt cgcgtaaagc gcaactgctg cgccgcgaag ctgaaaatga ggagaggtaa
     3661 tgttggatct gtttgccgat gctgaaccgt ggcaagagcc actggcggct ggtgcggtaa
     3721 ttttacggcg ttttgctttt aacgctgcag agcaactaat ccgcgatatt aatgacgttg
     3781 ccagccagtc gccgtttcgc cagatggtca cccccggggg atataccatg tcggtggcga
     3841 tgaccaactg tgggcatctg ggctggacga cccatcggca aggttatctc tattcgccca
     3901 ttgatccgca aacaaataaa ccgtggcccg ccatgccgca gagttttcat aatttatgtc
     3961 aacgtgcggc tacggcggcg ggctatccag atttccagcc agatgcttgt cttatcaacc
     4021 gctacgctcc tggcgcgaaa ctgtcgctgc atcaggataa agacgaaccg gatctgcgcg
     4081 cgccaattgt ttctgtttct ctgggcttac ccgcgatttt tcaatttggt ggcctgaaac
     4141 gtaatgatcc gctcaaacgt ttgttgttgg aacatggcga tgtggtggta tggggcggtg
     4201 aatcgcggct gttttatcac ggtattcaac cgttgaaagc ggggtttcat ccactcacca
     4261 ccgactgccg ctacaacctg acattccgtc aggcaggtaa aaaagaataa aaataagaat
     4321 tattattgct gtgcgcgcga agatgtttaa actgcgggct gtaattcatt gtccgggttt
     4381 tctgcatgga acttcttgta cttgtctggc ggcagtatcg ctggccattt atcagtgtga
     4441 tggcgctaag cctcgccagt gcggctttag gcattggctt aattgctttt atcaatcagc
     4501 gccttatcga aacggcggat accagtctgc tggtgttgcc ggagtttctg ggattattgc
     4561 tgctgttgat ggcagtcact ctcggatcgc aactggcgct caccactttg gggcatcact
     4621 tcgtttaccg actgcgtagt gaatttatca agcggattct ggatactcac gtcgagcgca
     4681 ttgaacaact cggtagcgcc tcgttgctgg cgggattaac cagcgatgtg cgcaatatca
     4741 ccattgcttt tgtgcgtctg ccggaactgg tgcaggggat cattctcact atcggttcag
     4801 cggcgtatct gtggatgctg tcgggcaaaa tgttgctggt aacggctatc tggatggcag
     4861 tcaccatctg gggcggtttt gtgctggtgg cgcgggtgta caaacatatg gcgaccctgc
     4921 gtgaaaccga agacaagctg tacacggatt ttcaaactgt acttgaaggg cgcaaagagc
     4981 tgactctgaa ccgggaacgc gccgagtatg tgtttaacaa cctctacatt cctgatgcgc
     5041 aagagtatcg ccatcatatt attcgcgcag acaccttcca tcttagtgcc gtgaactggt
     5101 caaacatcat gatgctgggc gctatcggcc tggtgttctg gatggcgaac agtctcggtt
     5161 gggctgatac caacgttgcc gcgacctatt cgttgacgct tttattcctg cgtacgccgc
     5221 tgctttcggc ggttggcgca ttgccgacgc tgctgacggc gcaggtggcg tttaacaagc
     5281 tgaacaaatt cgcgctcgcg cctttcaaag cagagtttcc gcgcccgcag gcgtttccca
     5341 gctggcaaac gctggagctg cgtaacgtga cgtttgctta tcaggataac gcgttttccg
     5401 ttggtccgat taatctcacc atcaaacgtg gcgagctgct gtttctgatt ggcggcaacg
     5461 gtagcggaaa atcgacgctg gcgatgttgt tgacgggctt gtatcagcca caaagcggcg
     5521 aaatcttgct ggatggcaaa cctgtcagcg gcgaacaacc ggaagattat cgcaaactgt
     5581 tttcggcagt gtttaccgat gtctggctgt ttgatcaact gctggggccg gagggtaaac
     5641 ccgctaaccc gcaactggtt gagaagtggc tggcgcagct gaaaatggct cataagcttg
     5701 agttaagaaa cgggcgtatt gttaacctga agttatcaaa agggcagaaa aaacgcgtgg
     5761 cgctgttgct ggcgctggca gaagaacgcg atattatcct gctggatgaa tgggcggcgg
     5821 atcaggatcc acacttccgt cgtgagtttt atcaggtgtt gctgtcgctg atgcaggaga
     5881 tgggtaaaac tattttcgcc atcagtcatg atgatcatta ctttatccac gccgaccgcc
     5941 tgctggaaat gcgcaacggg caacttagcg agctgacggg cgaagagcgc gatgccgctt
     6001 cgcgtgatgc cgttgcccgg acggcataat attacgacgc tgacctggcg gactgctgcc
     6061 gccaggtca
//
LOCUS       DAZKIF010000208         6021 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZKIF010000208 DAZKIF010000000
VERSION     DAZKIF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.209"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            240..686
                     /locus_tag="ACHOTW_004482"
     CDS             240..686
                     /locus_tag="ACHOTW_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFP9846451.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            697..2136
                     /locus_tag="ACHOTW_004483"
     CDS             697..2136
                     /locus_tag="ACHOTW_004483"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFP9846452.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2126..3196
                     /locus_tag="ACHOTW_004484"
     CDS             2126..3196
                     /locus_tag="ACHOTW_004484"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9846453.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3196..4944
                     /locus_tag="ACHOTW_004485"
     CDS             3196..4944
                     /locus_tag="ACHOTW_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFP9846454.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4994..6019)
                     /locus_tag="ACHOTW_004486"
     CDS             complement(4994..6019)
                     /locus_tag="ACHOTW_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFP9846455.1"
                     /translation="MVAIMALGLTGCDDKKAETETPLPANSQPAAPAPEAKPTEAPVA
                     KADAKPETPAQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLKDFKQGPTG
                     KESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMD
                     KYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEE
                     REGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNF
                     SFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMV
                     DDYNSLR"
BASE COUNT         1424 a         1526 c         1499 g         1572 t
ORIGIN      
        1 agccacaaat ataaataacg tttagacaaa ttatctgaga catttaaaca ttcacaaaat
       61 cattaaataa taactcttta ttcacaagat ttattttacc tactaatatg tgccttacgc
      121 cgcaaaattt atccttcact ctgctttata ttgtcacgac ttcacgacat atccgcgctc
      181 atcacaaacc cgccccgcta ttacaattaa ctgaaatatt ttttcatgga gtccgattaa
      241 tggcagctct tactgcaagc tgtattgacc tgaatattca gggcaatggc gcttattccg
      301 ttctgaagca gttggcgaca atggcgttac aaaacggttt tatcaccgac tcacaccagt
      361 tcctgcaaac cctgctgctg cgcgaaaaaa tgcactccac cggttttggt tctggtgtcg
      421 ccgtgccgca cggtaaaagc acctgcgtta aacaaccgtt cgtgttattc gcccgcaaag
      481 cgcaggctat tgactggaaa gccagcgacg gcgaagacgt caattgctgg atctgcctcg
      541 gcgtaccgca aagtggcgaa gaggatcagg tcaaaatcat cggcacactg tgtcgcaaaa
      601 ttattcacca ggaatttatt catcaactgc aacagggcga taccgaccag gtgcttgcct
      661 tgttaaatca aaccctcagc tcataaggaa gtggcgatgg agtcatcctt acgtattgtc
      721 gcgatcacca actgccccgc cgggatcgct cacacctaca tggtggcgga agccctggaa
      781 cagaaagccc gttctctcgg ccataccatt aaagtggaaa ctcaagggtc cagcggcgtt
      841 gaaaaccgct tatccagcga agagattgcc gctgccgatt acgtgattct cgccaccggg
      901 cgtggcctga gcggtgacga tcgcgcacgg tttgccggga aaaaagttta tgagattgcc
      961 atctcccagg cgctgaaaaa tatcgatcag attttcagtg aattaccgac aaattcgcag
     1021 ctttttgccg cagatagcgg cgtgaagcta ggcaaacagg aagtacaaag cggcagcgtg
     1081 atgagccacc tgatggcggg cgtctctgcg gcgctgccgt ttgtcatcgg cggtggtatc
     1141 ctggtggcgc ttgccaacat gctggtgcag ttcggtttgc cgtataccga tatgtcgaaa
     1201 ggcgcgccgt cgtttacctg ggtagttgaa tccatcggtt accttggctt cacctttatg
     1261 atccccatca tgggcgctta tattgcctcg tcgattgccg ataaaccggc tttcgctccg
     1321 gcgtttctgg tttgctatct ggcgaacgac aaagcgctgc tcggcaccca gtcgggtgcg
     1381 ggattcctcg gcgcggtggt gctggggctg gcgattggct atttcgtctt ctggttccgt
     1441 aaagtccgtc ttggcaaagc attacaaccg ctgctcggct ccatgcttat cccattcgtt
     1501 actctgctgg tttttggcgt gctcacttac tacgttatcg ggccggtgat gtccgacctc
     1561 atgggcgggc tactgcactt cctgaatacc attcctccat caatgaagtt tgcggcagcg
     1621 tttctggtcg gtgcgatgct ggcgttcgat atgggcggcc ccatcaataa aaccgcctgg
     1681 ttcttctgct tctcactgct ggaaaaacac atttatgact ggtacgccat cgtcggcgtt
     1741 gtccccgtcg cggcgggtct tgcgacgttc attgcgccca aactgtttac ccgacaggaa
     1801 aaagaggctg ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga accggctatt
     1861 ccttacgctc tggcagctcc gttgccgatg attaccgcca atacgctggc gggcggcatt
     1921 accggcgtgc tggtgatcgc tttcggaatc aaacgtctgg cacctggttt gggtatcttt
     1981 gacccgctga tcggcctgat gtcgccggta ggttcgtttt atctggtgct ggcgattggc
     2041 ctcgcgctga acatttcatt cattatcgtc ctgaaaggat tgtggttacg ccgcaaagcg
     2101 aaagccgcgc aacaggagct tgtccatgaa cattgagtta ctgcaacagt tgtgcgaagc
     2161 cagcgccgtc agcggcgatg aacaggaagt tcgcgacatt ctgataaaca cgctggaacc
     2221 ttgcgttaat gagatcacct ttgatggtct gggcagcttt gttgcccgta agggaaataa
     2281 aggtccaaaa gttgccgttg tcgggcatat ggatgaagtc ggctttatgg tcacccacat
     2341 cgacgagagc ggttttctgc gctttaccac cattggcggc tggtggaatc agtcgatgct
     2401 caaccaccgg gtaacaatac gcacacacaa gggagtgaaa atccctggtg tgattggttc
     2461 cgtagcgcct catgcgttaa cggaaaagca aaagcaacaa ccgctgtcat ttgatgagat
     2521 gttcattgat attggcgcga acagtcgcga agaagcgaaa aaacgcggtg cggaaattgg
     2581 cgattttatt agcccggaag ccaattttgc ctgctggggc gaagataaaa tagtcggcaa
     2641 ggcgctggat aatcgcatcg gctgcgcgat gatggctgag ctactacaga cagtaaataa
     2701 cccaggaatt acgctgtacg gcgtcggcag cgtggaagaa gaagttgggc tacgcggggc
     2761 acaaacctcg gctgaacaca ctaaaccgga tgtggtgatc gtgctggata ccgccgtcgc
     2821 gggcgatgtt ccgggcattg ataacattaa atacccgctg aaactgggca acgggccggg
     2881 gctgatgctg tttgacaagc gctacttacc caaccagaaa ctggtggcgg cgttaaaaaa
     2941 ctgtgccgca cataacggtt taccgctgca attttccacc atgaaaaccg gagcgacgga
     3001 tggcgggcgc tacaacgtaa tgggcggagg gcgtccggtt gtcgcgctgt gtctgccaac
     3061 tcgttatctg cacgctaaca gcggtatgat ttcaaaagcc gattatgatg ctctgctcac
     3121 gctgatacgg gattttctga cgaccttaac tgcggagaaa gtcaacgcgt ttagccagtt
     3181 ccgtcaggtg gattaatgct aaacgaacgc cagttaaaga ttgtcgatct gctggagcaa
     3241 cagccgcgca cgcctggcga gctggcgcaa cagactggcg tttcaggcag gaccatcctg
     3301 cgtgatattg actatctcaa ctttaccctt aacggcaaag ctcgtatctc tgccagtggc
     3361 agtgcgggct atcagttgga aatcttcgag cgccgcagct tttttcagtt gctgcaaaag
     3421 cacgataacg acgatcggct gctggcgctg ttattactga atacctttac tccccgtgcg
     3481 caactcgcct cggcgcttaa tttgccagaa acttgggtcg cagagcgtct gccccggtta
     3541 aaacagcgtt atgaacgcac ttgttgcctg gccagtcgtc ccggtttggg ccatttcatt
     3601 gatgaaacag aagagaaacg cgttatcttg ctggcgaact tgctacgcaa agatccgttt
     3661 ttaatacctt tggctggcgt aacacgagac aaccttcagc atttatccac ggcctgcgac
     3721 aaccaacacc gctggccgct catgcagggg gattatctct ccagcctgat tctggcgatt
     3781 tacgccctgc gtaatcaact gaccgatgag tggccgcaat atcccggtga cgagataaaa
     3841 caaatcgttg aacataacgg tctgtttctt ggtgataacg ctgtaagaac cctgacaggt
     3901 ttgatagaga aacagcatca gcaagcgcag gtaatttctg ccgatcatgt gctggggttg
     3961 ctgcaaaggg tgccaggtct cgcgtcattg aatattattg atacgcagct ggttgagaac
     4021 attaccggtc atttattacg ttgccttgcc gcaccagtgt ggattgctga gcatcgccaa
     4081 agcagcatga ataacctgaa agccgcctgg cctgcggcct ttgatatgag tctgcacttt
     4141 attacgctac tgcgtgaaca gctcgatatt ccccttttcg acagcgatct gatcggtttg
     4201 tattttgcct gtgcgctgga acgacatcaa aacgaacgcc agccgatcat tttgctctcg
     4261 gaccagaacg cgattgccac tattaatcag ctcgccattg agcgcgatgt tttacattgt
     4321 cgggtgatta ttgcccgtag cttaagcgaa cttgttgcca ttcgcgaaga gattgagccg
     4381 ttattgatca ttaacaacag ccattattta ctggatgacg cggtaaataa ttacatcacc
     4441 gtaaaaaata tcattacggc tgccggtatc gaacaaataa aacattttct ggcgacggca
     4501 tttattcgcc agcaaccgga gcgttttttc tctgcccccg gaagttttca ttattcgaat
     4561 gtacgcggtg aaagctggca acatattacc cggcaaattt gtgcgcaatt agtcgcacaa
     4621 caccatatta ccgccgatga agcgcaacgg atcatcgccc gcgaaggcga aggtgaaaac
     4681 ctgattgtta atcgcctcgc catcccacat tgctggagcg aacaggagcg acgttttcgt
     4741 ggatttttta ttaccctcgc ccaaccagtt gaggtgaata acgaagtcat taaccatgtc
     4801 ttgatcgcct gcgccgccgc cgatgcgcgt catgagctaa aaatatttag ctatctggca
     4861 agcgtattgt gtcagcatcc ggcagaagtt attgccgggc taacaggata tgaggcattt
     4921 atggagttac ttcacaaggg gtgaactgtg tcggatgcgc cgcatccgac actgcattta
     4981 tcatcaaaaa cgtttaacgc aggctattat agtcatcaac catttcgttg tactcgcgta
     5041 acgctcgtgg aaatgagtct tcgaccatcc agcttgtgtt tgcatcctga agatgctctt
     5101 tatcccagtc agagtacggg actttatctc tgacgcggcg aacatatttt ttagtctcga
     5161 gttgatactg gtctgccgaa ttaatgaacg agaaattcat gccgccttta tccgcttctt
     5221 tggcctgcgc caccagcgtt tccagttcag acactttctt cattgccgca tcaacatcaa
     5281 acttctcttg cgatatcagg ttattaattt gtttcgctga aatcatgact gccagagaat
     5341 agtagtggaa tgttttccct tctctttctt caatattttt cagttccgca agctggcgct
     5401 tatcattaat ttcctgaatc gccgcatgat aagattctgc tacaggttca aaggcttcca
     5461 gattttttaa gaatgtctgg tgcagcgttt tccctttcgc aaacgcatcg tctttataat
     5521 tttcctgggt gtaatattta tccatttcgt taatggtatt acccaacgcc acggcagcat
     5581 cgatataact caccgccacg ccatcaattg gttgcagcgc aggcgttaac gccacagcac
     5641 tttttacgcc tttttcacac tcagcgaggt tggattcact aatgccgtag atgccataaa
     5701 cagtactctc tttaccggta ggcccctgtt taaaatcttt cagccagtca gcataacgcg
     5761 ccagactgcg ctgtaccggg atctgtaact tgttgtagca cttgatatag acatccattt
     5821 tttcgtcgaa aaccgcttgt tcatcgacca gcggttgcgc aggtgtttca ggtttagcat
     5881 ctgcttttgc aacgggagct tcagtaggtt tcgcttcagg agctggtgcg gcaggttgac
     5941 tattggcagg caggggcgtt tctgtttcgg cttttttatc atcacaaccc gtcagaccga
     6001 gggccatgat ggcgactata a
//
LOCUS       DAZKIF010000209         5985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZKIF010000209 DAZKIF010000000
VERSION     DAZKIF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.210"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..253
                     /locus_tag="ACHOTW_004487"
     CDS             <1..253
                     /locus_tag="ACHOTW_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9846456.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            265..843
                     /locus_tag="ACHOTW_004488"
     CDS             265..843
                     /locus_tag="ACHOTW_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9846457.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            840..1235
                     /locus_tag="ACHOTW_004489"
     CDS             840..1235
                     /locus_tag="ACHOTW_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9846458.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1243..1983
                     /locus_tag="ACHOTW_004490"
     CDS             1243..1983
                     /locus_tag="ACHOTW_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9846459.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1999..2421
                     /locus_tag="ACHOTW_004491"
     CDS             1999..2421
                     /locus_tag="ACHOTW_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9846460.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2403..2837
                     /locus_tag="ACHOTW_004492"
     CDS             2403..2837
                     /locus_tag="ACHOTW_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9846461.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2830..5379
                     /locus_tag="ACHOTW_004493"
     CDS             2830..5379
                     /locus_tag="ACHOTW_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9846462.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5376..5705
                     /locus_tag="ACHOTW_004494"
     CDS             5376..5705
                     /locus_tag="ACHOTW_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9846463.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5705..>5985
                     /locus_tag="ACHOTW_004495"
     CDS             5705..>5985
                     /locus_tag="ACHOTW_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9846464.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAE"
BASE COUNT         1394 a         1468 c         1956 g         1167 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgt gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc
      181 gacggatgat ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
      241 ccgtcgtcgc tgaagggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac
      301 ctcagtcgta tcagcaaaac ggcggtgcct ggtgcgtcag caatggccat taaccgcgtt
      361 gcttcatccg cgatatcgca gtcggcgtca caggttgccc gtgagacaag agtacgccgg
      421 aaactggtaa aggaaagggc caggctgaaa agggccacgg ttaaaaatcc gcaggccaga
      481 atcaaggtta accgggggga tttgcccgta atcaagctgg gtaacgcgcg ggttgtcctg
      541 tcccgacgca ggcgtcgtaa aaaggggcag cgttcatccc tgaaaggtgg cggcagcgtg
      601 cttgtggtgg gaaaccgtcg tattcccggc gcgtttattc agcaactgaa aaatggccgg
      661 tggcatgtca tgcagcgtgt ggccgggaaa aaccgttacc ccattgatgt ggtgaaaatc
      721 ccgatggcgg tgccgctgac cacggcgttt aaacagaata ttgagcggat acggcgtgaa
      781 cgtcttccga aagagctggg ctatgcgctg cagcatcaac tgagaatggt aataaagcga
      841 tgaaacatac tgaactccgt gcagccgtac tggatgcact ggagaagcat gacaccgggg
      901 cgacgttttt tgatggtcgc cccgctgttt ttgatgaggc ggattttccg gcagttgccg
      961 tttatctcac cggcgctgaa tacacgggcg aagagctgga cagcgatacc tggcaggcgg
     1021 agctgcatat cgaagttttc ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga
     1081 tggagtcccg gatttatccg gtgatgagcg atatcccggc actatcagat ttgatcacca
     1141 gtatggtggc cagtggctat gactaccggc gcgacgatga tgcgggcctg tggagttcag
     1201 ccgatctgac ttatgtcatt acctatgaaa tgtgaggacg atatgccaac accaaatcct
     1261 ctggcaccgg tgaaaggggc cgggaccaca ctgtgggttt ataacgggaa cggcgaccca
     1321 tatgcaaacc cgctttcaga caatgactgg tcgcgtctgg caaaggttaa agacctgacg
     1381 cccggcgaac tgaccgctga gtcctatgac gacagttatc tcgatgatga agatgcggac
     1441 tgggccgcga ccggacaggg gcagaaatcc gctggagata ccagcttcac gctggcgtgg
     1501 atgcccggag agcaggggca gcaggcgctg ctggcgtggt ttaatgaagg tgatacccgt
     1561 gcctataaaa tccgcttccc gaacggcacg gtcgatgtgt tccgcggctg ggtcagcagt
     1621 atcggtaagg cggtgacggc gaaggaagtg atcacccgca cggtgaaagt caccaacgtg
     1681 ggacgtccgt cgatggcaga agatcgcagc acggtaacag cggcaaccgg catgaccgtg
     1741 acgcctgcca gcacctcggt ggtgaaaggg cagagcacga cgctgaccgt ggcattccag
     1801 ccggaaggcg caaccgacaa gagcttccgt gcggtgtctg cggataaaac aaaagccacc
     1861 gtgtcggtca gtggtatgac catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt
     1921 ccggtcgtat ccggtaatgg tgagtttgct gcggttgcag aaatcaacgt caccgccagt
     1981 taatccggag aatcagcgat gttcctgaaa accgaatcat ttgaacataa cggcgtgacc
     2041 gtcacgcttt ctgaactgtc agccctgcag cgtattgagc atctcgccct gatgaaacgg
     2101 caggcagaac aggcggagtc agacagcaac cggaagttta ctgtggaaga cgccatcaga
     2161 accggcgctt ttgtggtggc gatgtccctg tggcataacc atccgaagaa gacgcagatg
     2221 ccgtccatga atgaagccgt taaacagatt gagcaggaag tgcttaccac ctggcccgcg
     2281 gaggcaattt ctcatgctga aaacgtggtg taccggctgt ccggtatgta tgagtttgtg
     2341 gtgaatgatg cccctgaaca ggcagaggac gccgggcctg cagagcctgt ttctgcggga
     2401 aagtgttcga cggtgagctg agttttgccc tgaaactggc gcgcgagatg gggcgacccg
     2461 actggcgtgc catgcttgcc gggatgtcat ccacggagta tgccgactgg caccgctttt
     2521 acagtaccca ttattttcat gatgttctgc tggatatgca cttttccggg ctgacgtaca
     2581 ccgtgctcag cctgtttttc agcgatccgg atatgcatcc gctggatttc agtctgctga
     2641 accggcgtga ggctgacgaa gagcctgaag atgatgtgct gatgcagaaa gcggcagggc
     2701 ttgctggagg cgttcgtttt ggcccggacg ggaatgaagt tatccccgct tccccggatg
     2761 tggcggacat gacggaggat gacgtaatgc tgatgacagt atcagaaggg atcgcaggag
     2821 gagtccggta tggctgaacc ggtaggcgat ctggtcgttg atttaagtct ggatgcggcc
     2881 agatttgacg agcagatggc cagagtcagg cgtcattttt ccggtacgga aagtgatgcg
     2941 aaaaaaacag cggcagtcgt tgaacagtcg atgagccggc aggcgctggc tgcacagaaa
     3001 gcgggaattt ccgtcgggca gtataaagcc gccatgcgta tgctgcctgc gcagttcact
     3061 gacgtggcca cgcagcttgc aggtgggcaa agtccgtggc tgatcctgct gcaacagggg
     3121 gggcaggtga aggactcctt cggcgggatg atccccatgt tccgggggct tgccggtgcg
     3181 atcaccctgc cgatggtggg ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat
     3241 gcctggtatc agggcaactc aaccctgtcc gatttcaaca aaacgctggt cctttccggc
     3301 aatcaggccg ggctgacggc agatcgtatg ctggtcctgt ccagagccgg gcaggcggca
     3361 gggctgacgt ttaaccagac cagcgagtca ctcagcgcac tggttaaggc gggggtaagc
     3421 ggtgaggctc agattgcgtc catcagccag agtgtggcgc gtttctcctc tgcatcaggc
     3481 gtggaggtgg acaaggtcgc tgaagccttc gggaagctga ccactgaccc gacgtcgggg
     3541 ctgacagcga tggcgcgcca gttccataac gtgacggcgg agcagattgc gtatgttgct
     3601 cagttgcagc gttccggcga agaagccggg gcattgcagg cggcgaacga ggccgcgacg
     3661 aaagggtttg atgaccagac ccgccgcctg aaagagaaca tgggcacgct ggaaacctgg
     3721 gcagacagga cagcacgggc attcaaatcc atgtgggatg cggtgctgga tattggtcgt
     3781 cctgataccg cgcaggagat gctgattaag gcagaggccg cgtttaagaa agcggacgat
     3841 atctggaatc tgcgcaagga tgattatttt gttaacgatg aagcgcgggc gcgttactgg
     3901 gatgatcgtg aaaaggcccg tcttgcgctt gaagccgccc gaaagaaggc tgagcagcag
     3961 agtcaacagg acaaaaatgc gcagcaacag agcgataccg aagcgtcacg gctgaaatat
     4021 accgaagagg cgcagaaggc ttacgaacgc ctgcagacgc cgctggagaa atataccgcc
     4081 cgtcaggaag aactgaataa ggcactgaaa gacgggaaaa tcctgcaggc agattacaac
     4141 acgctgatgg cggcggcgaa aaaggactat gaagcgacgc tgaaaaagcc gaaacagtcc
     4201 ggcgtgaagg tgtctgcggg cgatcgtcag gaagacagtg ctcatgctgc cctgctgacg
     4261 cttcaggcag aactccggat gctggagaag catgccggag cgaatgagaa aatcagccag
     4321 cagcgccggg atttgtggaa ggcagaaagt cagttcgcgg tactggagga ggcggcacaa
     4381 cgtcgccagc tgtccgcaca ggagaaatcc ctgctggcgc ataaagacga gacgctggag
     4441 tacaaacgcc agctggctgt acttggcgac aaggtcacct atcaggagca cctgaacgcg
     4501 ctggcacagc aggcggataa attcgcacag caacaacggg caaaacgggc cgccattgat
     4561 gcaaaaaacc gggggctgac tgaccggcag gcagcgcggg aagccacgga acagcgcctg
     4621 aaggaacagt atggcgataa tcctctggcg ctgaataacg tcatgtcaga gcagaaaaag
     4681 acctgggcag ctgaagacca gcttcgcggg agctggatgg caggcctcag gtcaggctgg
     4741 agtgagtggg aagagagcgc cacggacagt atgtcgcagg ttaaaagtgc tgccacgcag
     4801 acctttgatg gtattgcaca gaatatggcg gcgatgctga ccggcagtga acagaactgg
     4861 cgcagcttca cccgttccgt gctgtccatg atgacagaaa ttctgcttaa gcaggcaatg
     4921 gtggggattg tcggaagtat aggcagcgcc attggcggcg gcgcatcagc gtcaggcggt
     4981 acagccattc aggcagctgc ggcgaaattc cattttgcgg ccggagggtt tacgggaacc
     5041 ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt cacgaaggag
     5101 gcaaccagcc ggattggcgt aggaaatctt taccggctga tgcgcggcta tgccaccggc
     5161 ggttatgtcg gtacaccggg cagcatggcg gacagccggt cgcaggcgtc cgggacgttt
     5221 gagcagaata accatgtggt gattaacaac gacggcacga acggtcagat agggccacag
     5281 gcactgaagg cggtttatga cgtagcccgt aaggcggcaa tggatgttgt gaccgggcag
     5341 atgcgcgatg gtggtctgtt ctccggaggt ggacgatgaa aaccttccgc tggaaagtga
     5401 aacccggtat ggatgtggct tcggcccctt ccgtaagaaa ggtgcgcttt ggtgatggct
     5461 attcccagcg agcgcctgcc gggctgaatg ccgacctgaa aacgtacagc gtgacgcttt
     5521 ctgttccccg ttgggaggcc acggcgcttg agtcgtttct ggctgagcac gggggctgga
     5581 aggcctttct gtggacgccg ccttatgagt ggcggcagat aaaggtgacc tgcgcaaaat
     5641 ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa caggtggtga
     5701 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
     5761 cagcgtggtg ctctgggaaa tcgatctgac agaggtcggt ggagaacgtt attttttctg
     5821 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
     5881 tcccattcag gggagtggtt ttgaactgaa tggcaaaggc accagtacgc gccccacgct
     5941 gacggtttct aacctgtacg gtatggtcac cgggatggcg gaaga
//
LOCUS       DAZKIF010000210         5902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZKIF010000210 DAZKIF010000000
VERSION     DAZKIF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.211"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            136..678
                     /gene="fimA"
                     /locus_tag="ACHOTW_004496"
     CDS             136..678
                     /gene="fimA"
                     /locus_tag="ACHOTW_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9846465.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            898..1590
                     /gene="fimC"
                     /locus_tag="ACHOTW_004497"
     CDS             898..1590
                     /gene="fimC"
                     /locus_tag="ACHOTW_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9846466.1"
                     /translation="MMTKIKLLMLIIFYLIISASVHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1621..4230
                     /gene="sfmD"
                     /locus_tag="ACHOTW_004498"
     CDS             1621..4230
                     /gene="sfmD"
                     /locus_tag="ACHOTW_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP9846467.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4243..5250
                     /gene="fimH"
                     /locus_tag="ACHOTW_004499"
     CDS             4243..5250
                     /gene="fimH"
                     /locus_tag="ACHOTW_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9846468.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5261..5776
                     /gene="sfmF"
                     /locus_tag="ACHOTW_004500"
     CDS             5261..5776
                     /gene="sfmF"
                     /locus_tag="ACHOTW_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFP9846469.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(5779..>5902)
                     /locus_tag="ACHOTW_004501"
     CDS             complement(5779..>5902)
                     /locus_tag="ACHOTW_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9846470.1"
                     /translation="ADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
BASE COUNT         1565 a         1252 c         1409 g         1676 t
ORIGIN      
        1 ttttagaata gagtgcatat tttcaattaa gacattctta gaggataaaa aggaatttac
       61 tactatcagt gtcttaaata aagtaatcgg ttatatacgg atgtggagtc gataaatgag
      121 attgaaggaa tatatatgaa attaagattt atttcgtctg cgctggctgc cgcactattc
      181 gccgctacgg gtagttatgc tgccgttgta gatggcggta caattcactt tgaaggtgaa
      241 ctggtgaatg ctgcctgttc agtgaatact gactcggcag accaggttgt cacactcggt
      301 caatatcgta ccgatatttt caatgctgtt ggtaatacct ctgcattaat tccattcacc
      361 attcagttga acgactgcga tcctgttgtt gccgctaatg ctgccgttgc attttctggt
      421 caggctgatg caatcaatga taatttattg gccattgcat ccagtaccaa tacaacaaca
      481 gcaacgggtg tcggtattga aatacttgat aatacatccg caattctcaa acctgatggg
      541 aatagcttct caaccaacca gaacttgatc cccgggacca acgttcttca tttttctgca
      601 cgttataaag gcaccggtac aagtgcatca gcagggcaag caaatgctga cgcgactttt
      661 attatgagat acgaataatc aaaaccacgt tgttttgaat tatatatcac gtcttataac
      721 aaagtaatgt accggttgtc tgaagcggta tggtggcaat gtaaatcgaa atcatgttca
      781 ctttgtatca tgccgcttta ttaaatgaaa agggaatgat gtgttgtaag aaaccaaagc
      841 aatcatttct ttatatttct tatttttgcc gtcaggaata cacaaggcgt attaactatg
      901 atgactaaaa taaagttatt gatgctcatt atattttatt taatcatttc ggccagcgtc
      961 catgctgccg gagggatcgc attaggtgcc acgcgtatta tttatcccgc tgatgctaaa
     1021 cagactgcgg tatggattag aaatagccat accaatgagc gttttctggt caattcgtgg
     1081 attgaaaaca gcagcggtgt aaaagaaaag tcatttatca ttacaccgcc actatttgtt
     1141 agtgaaccca aaagcgaaaa tactttgcgt attatttaca ccggtccacc gctggcagca
     1201 gatcgtgagt ctctgttctg gatgaatgtt aagacgattc cttcggtaga taaaaatgca
     1261 ttgaacggca ggaatgtttt gcaactggcg attttatcgc gcatgaagtt atttctccgt
     1321 ccaattcaac tacaagaatt acccgcagaa gcgccggata cactcaagtt ttcgcgatcc
     1381 ggtaactata tcaatgttca taatccatca cctttttatg tcaccctggt taacttacaa
     1441 gtgggcagcc aaaagttggg gaatgctatg gctgcaccca gagttaattc acaaattccc
     1501 ttaccctcag gagtgcaggg aaagctgaaa tttcagaccg ttaatgatta tggttcagta
     1561 accccggtca gagaagtgaa cttaaactaa ccgaatcatc tgacaatatc agagctaatt
     1621 atgaaaatac ccactactac ggatattccg cagaggtata cctggtgtct ggccggaatt
     1681 tgttattcat ctcttgccat tttaccctcc tttttaagct atgcggaaag ttatttcaac
     1741 ccggcatttt tattagagaa tggcacattc gttgctgatt tatcgcgctt tgagagaggt
     1801 aatcatcaac ctgcgggcgt gtatcgggtg gatctctggc gtaatgatga gttcattggt
     1861 tcacaggata tcgtatttga atcgacaaca gaaaatacag gtgataaatc aggtgggtta
     1921 atgccctgtt ttaaccaggt actccttgaa cgaattggcc ttaatagcag tgcatttccc
     1981 gagttagccc agcagcaaaa caataaatgc atcaatttac tgaaagctgt acctgatgcc
     2041 acaattaact ttgattttgc agcgatgcgc ctgaacatca ctattcctca gatagcgttg
     2101 ttgagtagcg ctcacggtta cattccgcct gaagagtggg atgaaggtat tcctgcttta
     2161 ctcctgaatt ataatttcac cggtaacaga ggtaatggta acgatagcta tttttttagt
     2221 gagctcagcg ggattaatat tggcccgtgg cgtttacgca acaatggttc ctggaactat
     2281 tttcgcggaa atggatatca ttcagaacag tggaataata ttggcacctg ggtacagcgc
     2341 gccattattc cgctgaaaag tgaactggta atgggagacg gcaatacagg aagtgatatt
     2401 ttcgatggtg ttggatttcg tggtgtacgg ctttattctt ccgataatat gtatcctgat
     2461 agccagcaag ggtttgcccc aacggtacgt gggattgccc gtacggcggc ccagctaacg
     2521 attcggcaaa atggttttat tatctatcaa agctatgttt cccccggcgc ttttgaaatt
     2581 acagatttgc acccgacatc ttcaaatggc gatctggacg tcaccatcga cgaacgcgat
     2641 ggcaatcagc agaattacac tattccgtat tcaacagtgc caattttaca acgcgaaggg
     2701 cgtttcaaat ttgacctgac ggcgggcgat tttcgtagcg gtaatagtca gcaatcatcg
     2761 cctttctttt ttcagggcac ggcactcggc ggtttaccac aggaatttac tgcctacggc
     2821 gggacgcaat tatctgccaa ttacaccgcc tttttattag ggctggggcg caacctcggg
     2881 aactggggcg cagtgtcgct ggatgtgacc catgcgcgca gtcagttagc cgacgacagt
     2941 cgtcatgagg gggattccat tcgcttcctc tatgcgaaat cgatgaacac cttcggcacc
     3001 aattttcagt taatgggtta ccgctattcg acacaaggtt tttataccct tgatgatgtt
     3061 gcgtatcgtc gaatggaggg gtacgaatat gattacgatt atgacggtga gcatcgggat
     3121 gaaccgataa tcgtgaatta ccacaattta cgctttagcc gtaaagaccg tttgcagtta
     3181 aatatttcac aatcacttaa tgactttggc tcgctttata tctctggtac ccatcaaaaa
     3241 tactggaata cttcggattc agatacctgg tatcaggtgg ggtataccag cagctgggtt
     3301 ggcatcagtt attcactctc attttcgtgg aatgaatctg tagggatccc cgataacgaa
     3361 cgtattgtcg gacttaatgt ttcagtgcct ttcaatgttc tgaccaaacg tcgctacacc
     3421 cgggaaaatg cgctcgaccg cgcttatgcc tcctttaacg ccaaccgtaa cagcaacggg
     3481 caaaatagct ggctggcagg tgtaggtggg accttactgg aaggccacaa cctgagttat
     3541 cacgtaagcc agggtgatac ctcgaataat gggtatacgg gcagcgccac ggcaaactgg
     3601 caggccgctt acggtacgct gggggtcggg tataactacg accgcgatca acatgacgtt
     3661 aactggcagc tgtctggcgg tgtggtcgga catgaaaatg gcataacgct gagccagcct
     3721 ttaggggata ccaatgtttt gattaaagcg cctggcgcag gcggtgtacg cattgaaaat
     3781 caaactggca ttttaaccga ctggcgcggc tatgcggtga tgccgtatgc cacggtttat
     3841 cggtataacc gtatcgcgct tgataccaat acgatgggga attccatcga tgttgaaaaa
     3901 aatattagca gcgttgtgcc gacgcaaggc gcgttggttc gtgccaattt tgatacccgc
     3961 ataggcgtgc gggcgctcat taccgttacc cagggcggaa aaccggtgcc gtttggatca
     4021 ctggtacggg aaaacagtac cggaataacc agtatggtgg gtgatgacgg gcaagtttat
     4081 ttaagtggtg cgccattgtc tggtgaatta ctggttcagt ggggagacgg cgcgaactca
     4141 cgctgcattg cgcactatgt attgccgaag caaagcttac agcaagccgt cactgttatt
     4201 tcggcagttt gcacacatcc tggctcataa aggaaattat caatgaagat aatctgcaga
     4261 ttattattgg cgatggcatg tttgtgtctg gcaaacatat cctgggctac tgtttgtgca
     4321 aatagtactg gcgtagcaga agatgaacac tatgatctct caaatgtctt taatagcacc
     4381 aataaccagc cagggcagat tgttgtttta ccggaaaaat ccggctgggt aggtgtctca
     4441 gcaatttgtc cacccggcac gctggtgaat tatacatacc gtagttatgt cactaacttt
     4501 attgttcagg aaactatcga taattataaa tatatgcaat taaatgatta tctattaggt
     4561 gcgatgagtc tggttgatag tgtgatggat attcaatttc ccccgcaaaa ttatattcgt
     4621 atgggaacag atcctaacgt ttcgcaaaac cttccgttcg gggtgatgga ttctcgtttg
     4681 atatttcgtt taaaggttat tcggcccttt attaacatgg tggagatccc cagacaggtg
     4741 atgtttaccg tgtatgtgac atcaacgcct tacgatccgt tggttacacc tgtttatacc
     4801 attagttttg gtggccgggt tgaagtaccg caaaactgcg aattaaatgc cgggcagatt
     4861 gttgaatttg attttggtga tatcggcgca tcgttattta gtgcggcagg gccgggtaat
     4921 cggcctgctg gtgtcatgcc gcaaaccaag agcattgcgg tcaaatgtac gaatgttgct
     4981 gcgcaggctt atttaacaat gcgtctggaa gccagtgccg tttctggtca ggcgatggtg
     5041 tcggacaatc aggatttagg ttttattgtc gccgatcaga acgatacgcc gatcacgcct
     5101 aacgatctca atagcgttat tcctttccgt ctggatgcag ctgcggcagc caatgtcaca
     5161 cttcgcgcct ggcctatcag tattaccggt caaaaaccga ccgaagggcc gtttagcgcg
     5221 ctggggtatt tacgcgtcga ttatcaatga ggtacggaga atgagaagag tattctttag
     5281 ctgtttctgc gggttactgt ggagttccag tggatgggca gctgaccctt taggaacgat
     5341 taatatcaat ttgcacggta acgttgttga tttctcctgt accgtaaaca cagcggatat
     5401 tgataagacg gtagatttag gcagatggcc tacgacacaa ttactgaacg ctggcgatac
     5461 cacggcactc ctccctttta gcctgcggct ggagggatgt cctccgggtt cagttgcgat
     5521 tttatttacg ggaacgccgg catctgatgc caacctgctg gctctggatg atcccgcaat
     5581 ggcacaaacc gtcgccatcg aattacgtaa tagcgatcgc tcccggctcg cactggggga
     5641 ggcgagcccg actgaggaag tagatgcaaa tggcaatgtc acactaaact tttttgccaa
     5701 ttatcgagcg ttagccagcg gtgttcggcc aggtgtggcg aaagcggatg cgatatttat
     5761 gatcaattat aattaatatt atattaattc gtataatttg gcgtagtcga taagctctac
     5821 aattgaatgc aaacctagct tgccataaat attagattta tgcgcactaa ctgttttatt
     5881 gctaagtaat aacttatcgg ca
//
LOCUS       DAZKIF010000211         5898 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZKIF010000211 DAZKIF010000000
VERSION     DAZKIF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.212"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            181..282
                     /locus_tag="ACHOTW_004502"
                     /pseudo
     CDS             181..282
                     /locus_tag="ACHOTW_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(692..927)
                     /locus_tag="ACHOTW_004503"
                     /pseudo
     CDS             complement(692..927)
                     /locus_tag="ACHOTW_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1086..1595)
                     /locus_tag="ACHOTW_004504"
     CDS             complement(1086..1595)
                     /locus_tag="ACHOTW_004504"
                     /inference="COORDINATES: protein motif:HMM:NF018156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9846471.1"
                     /translation="MDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLD
                     VFSTYMGQVISEFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQR
                     GVFLRNAEGSRVCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHF
                     TVRSDCYSV"
     gene            1968..3024
                     /locus_tag="ACHOTW_004505"
                     /pseudo
     CDS             1968..3024
                     /locus_tag="ACHOTW_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3243..3833)
                     /gene="espM1"
                     /locus_tag="ACHOTW_004506"
     CDS             complement(3243..3833)
                     /gene="espM1"
                     /locus_tag="ACHOTW_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFP9846472.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            4017..4664
                     /gene="nleG"
                     /locus_tag="ACHOTW_004507"
     CDS             4017..4664
                     /gene="nleG"
                     /locus_tag="ACHOTW_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFP9846473.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
     gene            complement(4801..4947)
                     /locus_tag="ACHOTW_004508"
     CDS             complement(4801..4947)
                     /locus_tag="ACHOTW_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846474.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
     gene            5375..5653
                     /locus_tag="ACHOTW_004509"
     CDS             5375..5653
                     /locus_tag="ACHOTW_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFP9846475.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
BASE COUNT         1805 a         1300 c         1079 g         1714 t
ORIGIN      
        1 aatttacgat gcttttttat acagatctct cgtgttttta actgattgat tttgttgaac
       61 tctttttttg ttttgcagga gttactcctc ttaccttatt gtccactcga tgaccaacac
      121 attcacaacc caaaccagaa tatcctatct atgaagcatg tatccgcacg cagattaatt
      181 atggtttaca ttgttctacc acaatagtcg catcctaaat gggtgcggtt gaggatcacc
      241 atccttgaag aggatgcccg ccactttaac tgcatgacag ggctatttaa acaatcacta
      301 aaaatcgccc aagagcattc tgtttctata atgtattcca agagattaaa aaataatatc
      361 atacgttatt cggcagagtt cgtatgtact acggcgtccc aaccatgtcc caaaacaaaa
      421 atttctgccc tatacacgtt ccaaaaaatt caacagcaaa gagagtctgc tgacgagttg
      481 ttgattcact gattaatacg taaaattgat aactgctgga aatcatacaa tactcgtact
      541 ttcgaaagtt cgctaaccag gtgctacacg cgaggtgttg cagttttctt tacgatttat
      601 cattcaatcc taatccatgc atgacatgtg gtgcctgtgg ggtgatccta cctacgtaat
      661 gtggacacag ccctaatcaa ggttctgttt ttcaaaacag ttccgtgctg agacagccac
      721 aagtactgtg tcggcaccac cgattgtaat cacactagat ctaattaaac acagccgtct
      781 gcattatttc ccgactcata aaatattagc cgtaaatgca ttcgactttt agtgagtgga
      841 agaaactttc cgccctagca ttatcgtaac agcgcccctt tgcactcatc ctgtcatgca
      901 ggtatgccgc ttcagaagcg cctgataatg cgttgaaatc aggacggtgg ctacttgcta
      961 ttcagttcac gatacatccc ttaaggacca gcgaatagca atgagtcact ttattatcaa
     1021 gtttgatgac cgctttgatg ctaactttta ataagagtct cttcacacaa aatcgtgtac
     1081 cgagtctaca ctgaataaca atcactcctc accgtaaagt gaccgatact gcaatcaaac
     1141 tcacactgct ctctggccac aagcattgat aatgttattg gctctctgct tagtgggtga
     1201 tgcatcccat cattaattat acgagaaaga gccatttcat catataaact acatactctg
     1261 gaaccttcag catttcttaa aaaaacacct cgttgaggaa ttgccagcgt aatcgggcac
     1321 tgtaatgctt catctatgta agacatttct tcaatattaa ctaaaaatga acatgaatgt
     1381 aatcgccgct gtaacaattc aattctgtta tcattactat gcataaattc agaaataacc
     1441 tgccccatat atgtactgaa tacatcaaga aatgaattgc ctccattcaa ttgattttca
     1501 acagaatcag ccaatctaat gatatcggct agttctgtat ggcttaaacc cgaatatagt
     1561 tccccttgaa caggatctac aataaaagcg tccatagcac tctgatagcg aacagaaaac
     1621 gatcattctc ctattgtgag tctttccatt tgatgatctc tggtcgcacg ttgtaaaact
     1681 tcaacatctt gtgttgacaa gtaatttcca gaagacaaat taaatgaaaa tggcataatt
     1741 ttatccctaa agcatatatc ttacttttcg ataattaaca aaaagccatc ctccacagca
     1801 atactccatc agaataattg taggattatt ttaatctaat ttataagcat aaaatatata
     1861 ctcactaaat agctgcgcag aatagtagat catttagagg gaactcaacc cgattgtgcg
     1921 atctgatcaa tcgccaaatc aatacaaatc accaaccgga ctgagcaatg tcgatcatac
     1981 caccaatttc ccgtgacgaa cgacgcctaa tacagaaagc catccataaa aggctgacag
     2041 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgcccgcaca ctatgctgcg
     2101 cccgttcctc tgttgggcgc tggattaact agttcacaca gtcgggtgtt gagggactga
     2161 aatcattacc tgccgagcga gcccgccgct ggccgtttgc gcatatctac acactattac
     2221 gtgaacaggt aaaatattca ctcgcgactt tggctacaat tattcacgct ggagtacaga
     2281 actgctgaca ataaaaatca atgagataac cggctgcctg ttacatgctg gaaccattcg
     2341 ccgctggttg tcgtctgtgg ggcttgtgtg gcgaagggct gcgccaactc tacgtatccg
     2401 tgccccgcct aaagataaaa agatggcaac aatccataac gcactggacg aatgcagcac
     2461 agagcatccg gtcttttatg aagatgaagt atttatccat cttaatccca aaatcggtgc
     2521 ggactggaaa ctgctcggga agcaaaaacg aggggtcacg ccggaacaaa atgaaaaata
     2581 ttctctggac gtagcgctgc acagcgggac aggttaagtc agctgtgtgg gcgccaacag
     2641 caaaagttcg gcgctgttca tcagactgct gaagcggctt aaagctacat actgtcggac
     2701 gaaaaccatc acgctgctcg aggacaacta cattatccac aaaggccggg aaacccagcg
     2761 ctggctgaag gataaaccga agtgcagagt catttatcag ccggtttaat ctccatgggt
     2821 gaatcatttt gaatagctat ggcagatact tcacgccaca ataacgcgta atcatcagtg
     2881 ccgctcaatg tggcaactat tgaaaaagtt cgccatttta tggaatccgt cagtccattc
     2941 cccgaaggca aacatgggct ggcaaaaatg tgcgatatta ggcgcagcta tttagttatt
     3001 ccgtcatttc tcccgcccgt gtaaacacta ctgaatcttg tcgttgtgac aaagctataa
     3061 atagtcaatc acagcacgtt tctgcgtacg acgcgctgca attttcttta cgatttataa
     3121 ctgtacaggt gaactatgag ttatttggat acatacatat agtgtaacgc ctgttgtata
     3181 ttttactcat atctttttac aggatgaaaa ccaatgatac tggcgcatag cctcctccaa
     3241 ccttacccct gtataacacg actcatataa cctgggattt catttcgtag ttttacaata
     3301 tcaacacccg gggcgatatg tttctgataa aacttgtctg caagcacgtt tgctatgtaa
     3361 tctgttgtac tgttttttac agctcggctc ataccatcgc aaagagcatc catttttttg
     3421 ccaaaagaag catttcctat tattatatga ttaatagaac tttgtgccgc tttaacatca
     3481 agagttagtt caaactcctt acttacaaga gaaaagacca ttttcctctc ttcagaagaa
     3541 atttttgcat ttttttgaag gcagcaatta tcaatctctt ggttgataac ccttgacgga
     3601 tatactgtcc gttcatcact tacccatcca tgaatatctt tagtactctg cctgcttacc
     3661 gtctccagta tacagccgct aaatttgtta acattaaagc ggatagagcg ctctcccatt
     3721 gaacacttca cgacctcatc attcttccca cggatggtgc cccggaaaga attatcttta
     3781 tgataactga taccaaaaga gctgaaggac acacctgtcg catttactgg cataacaaat
     3841 ccttttcatt aaaatttgct tacaaaaatt gtatacaatc attttaatta aaaaaataca
     3901 tgtgtaaata gcattcgttc aaaatttaat tttttgtttg aatcctatca catatttagt
     3961 atcttgactc ctgtcaccaa tatggttcaa taacatagac taaagaggga gaaaatatgc
     4021 ctgtcatatt aaacttttcg agtgaaagag tattgtctga aagtgaactg gaggctttac
     4081 gtcatgtcgg aagagttagt cagagtgaac agcttgttgt gagaggccgc actatgagac
     4141 ttcatcatat ttcatttatg gacagtttta gcgtggaacc ggtttccggc ggactgttgg
     4201 atcgccttag tgcaagaggg catcgccttc tcgcagaaaa tctggagata cagcttaaca
     4261 gaggccatac ttttttgcag gcattccgtt tatatatgga acagagccgt gcaaccccat
     4321 gtacgcgcca aaacgtcagt agtgcaatac aaaacaaaat taactctcat gcatttactg
     4381 ttagtcatca agacttttcc tgtcatgaac aacacctgaa ttgcccgata acattatgca
     4441 ttcctgaaac tggtgttttt gtcagaaatg caaaaaactc agaaatatgt tcattatatg
     4501 accacaatgc actcactgaa cttatccgac gcaacgctcc acaccctctc agccgtgaac
     4561 cctttgttcc ggaaatgatt gtcagcaaag atgaatgcca ttttaatcta atagaacaat
     4621 atttttgcat attagccaca caaaatatat gcactagaat ttgactgtat ggttcgattt
     4681 aaatgttgca aaattaattt tactagttat tgccatctat ttcatactcc ctaattcttg
     4741 atacaatctg tctgtcaaaa tagtagatta gatcgaactt atattacagc tatttagttt
     4801 ctaaaatgga atttttaacg ttttatactt cacattacaa tctgtaccat aagcctcctc
     4861 agcgaggttg cctgaatacc ctgtcgtgcc catttttcca tcggacgcag ttgtctgctc
     4921 tctcgaacta ctggcaataa aaatcaacca gacagcttga tgtccgttac atgcaggact
     4981 aatcacgatt ggttaccgtt tacaaaactt gcgtaacatt ggaggagaac gaataatatt
     5041 ctctagctgg tacgcgttcc atagcgaaac gggcaaggtc caacagcaag agctgaaact
     5101 cagggggatt tatcagtcga tttattcgcc atggatgaat cacgctgtac ggttatggta
     5161 gctcttagcg acaaggcata atcatcagta caccaatgta acaaccgttg aaaaagactc
     5221 attattttat gaaccagcca tcccatttcc agagtggtaa atatgatttg ttaaagtgta
     5281 acagtactct gcgtagcctt tttagattca tagtttaatg tttctaaata cattaaattg
     5341 cactataaga gaaagacata atgtgaggat aaaaatgcca ttttcaataa aaagtatttt
     5401 ttcaggacac acttggcatc aacctgaaat atctaggcct atagcagata aatcatctac
     5461 taaaaattgt atcttagact caacaacatg caatgtagat ggctttacag tgtttaacag
     5521 aagatcctgt agttttgata tgcgtcctcc cggaagcgca gacagaactc cacaactgag
     5581 gttatcaata tcagaagtcg catggatgtc aaaaataata gagacagaga caaataatac
     5641 aaacaaatca tagctattag cccagaaaag agttggtaca ctagccatga ataacttcct
     5701 aacaacttga ttttttagtt atttttttct tcattcttga aaaaggatgc cccgaaaggg
     5761 ttccagagtt cgattttctg cgcttctgca aataatcaag gggttacaaa gtggttgccc
     5821 ctttgttttt agtgctcttg gtatacccat tggtacattt agcgcaatac catctggtat
     5881 cacttaaaga cacgaaaa
//
LOCUS       DAZKIF010000212         5879 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZKIF010000212 DAZKIF010000000
VERSION     DAZKIF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.213"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            131..778
                     /gene="fucA"
                     /locus_tag="ACHOTW_004510"
     CDS             131..778
                     /gene="fucA"
                     /locus_tag="ACHOTW_004510"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP9846476.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            806..1954
                     /gene="fucO"
                     /locus_tag="ACHOTW_004511"
     CDS             806..1954
                     /gene="fucO"
                     /locus_tag="ACHOTW_004511"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9846477.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2009..2764)
                     /gene="xni"
                     /locus_tag="ACHOTW_004512"
     CDS             complement(2009..2764)
                     /gene="xni"
                     /locus_tag="ACHOTW_004512"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP9846478.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2876..4243)
                     /gene="sdaB"
                     /locus_tag="ACHOTW_004513"
     CDS             complement(2876..4243)
                     /gene="sdaB"
                     /locus_tag="ACHOTW_004513"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP9846479.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4301..5590)
                     /gene="sdaC"
                     /locus_tag="ACHOTW_004514"
     CDS             complement(4301..5590)
                     /gene="sdaC"
                     /locus_tag="ACHOTW_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP9846480.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1470 a         1473 c         1571 g         1365 t
ORIGIN      
        1 tcacaaaata taagcttgtg aatttctttc ttgttaatca ggaagaagat ggtgggtaat
       61 taaacggcta attcaatagt gtgaaaggaa caacattatt gccctgtttt gaatcagaga
      121 gaggtagcta atggaacgaa ataaacttgc tcgtcagatt attgacactt gcctggaaat
      181 gacccgcctg ggactgaacc aggggacagc ggggaacgtc agtgtacgtt atcaggatgg
      241 gatgctgatt acgcctacag gcattccata tgaaaaactg acggagtcgc atattgtctt
      301 tattgatggc aacggtaaac atgaggaagg aaagctcccc tcaagcgagt ggcgtttcca
      361 tatggcggcc tatcaaagca gaccggatgc caacgcggtt gttcacaatc atgccgttca
      421 ttgcacagca gtttccattc ttaaccgacc gatccccgct attcactaca tgattgcggc
      481 ggctggcggt aattctattc cttgcgcgcc ttatgcgacc tttggaacac gcgaactttc
      541 tgaacatgtt gcgctggctc tcaaaaatcg taaggcaact ttgttacaac atcatgggct
      601 tatcgcttgt gaggtgaatc tggaaaaagc gttatggctg gcgcatgaag ttgaagtgct
      661 ggcgcaactt tacctgacga ccctggcgat tacggacccg gtgccagtgc tgagcgatga
      721 agagattgcc gtagtgctgg agaaattcaa aacctatggg ttacggattg aagagtaatt
      781 tcgtaaagca acaaggagaa ggatgatggc taacagaatg attctgaacg aaacggcatg
      841 gtttggtcgg ggtgctgttg gggctttaac cgatgaggtg aaacgccgtg gttatcagaa
      901 ggcgctgatc gtcaccgata aaacgctggt gcaatgcggc gtggtggcga aagtgaccga
      961 taagatggat gctgcagggc tggcatgggc gatttacgac ggcgtagtgc ccaacccaac
     1021 aattactgtc gtcaaagaag ggctcggtgt attccagaat agcggcgcgg attacctgat
     1081 cgctattggt ggtggttctc cacaggatac ttgtaaagcg attggcatta tcagcaacaa
     1141 cccggagttt gccgatgtgc gtagcctgga aggactttcc ccgaccaata aacccagtgt
     1201 accgattctg gcaatcccca ccacagcagg cactgcggca gaagtgacca ttaactacgt
     1261 gatcactgac gaagaaaaac ggcgcaagtt tgtttgcgtt gatccgcatg atatcccgca
     1321 ggtggcgttt attgacgctg acatgatgga tggtatgcct ccagcgctga aagctgcgac
     1381 gggtgtcgat gcgctcactc atgctattga ggggtatatt acccgtggcg cgtgggcgct
     1441 aaccgatgca ctgcacatta aagcgattga aatcattgct ggggcgctgc gaggatcggt
     1501 tgctggtgat aaggatgccg gagaagaaat ggcgctcggg cagtatgttg cgggtatggg
     1561 cttctcgaat gtggggttag ggttggtgca tggtatggcg catccactgg gcgcgtttta
     1621 taacactcca cacggtgttg caaacgccat cctgttaccg catgtcatgc gttataacgc
     1681 tgactttacc ggtgagaagt accgcgatat cgcgcgcgtt atgggcgtga aagtggaagg
     1741 tatgagcctg gaagaggcgc gtaatgccgc tgttgaagcg gtgtttgctc tcaaccgtga
     1801 tgtcggtatt ccgccacatt tgcgtgatgt tggtgtacgc aaggaagaca ttccggcact
     1861 ggcgcaggcg gcactggatg atgtttgtac cggtggcaac ccgcgtgaag caacgcttga
     1921 ggatattgta gagctttacc ataccgcctg gtaaatgcgc tgatgtgata atgccggatg
     1981 cgacgtttgc ggcgtatccg gcacgccgtt accgtaccaa ccgcaattgc tgtaaattgc
     2041 cgtcgatatg caaatcggtt tgtaagcggg caatatcgcg gcacagaaac gccatctctt
     2101 tatgggtttc taattttttg cgccactttt cggcaaccgc atccagattc tcatatatcc
     2161 cttccagact ctgaaactcg accagcagct gcgtggcgct ttttggtccg attcccgcaa
     2221 cacccggtac ctttgaactg ctgatccccg caagtcccca gtaatcgggc aactgctgcg
     2281 gttgaacgcc aaattcttta tcgataaatg gcgcatccag ccaacgtttc tggaagtaat
     2341 cacgaatacg taatgtcggt gaaagtaact gacagtagcc tttatctgtc gaaacaatcg
     2401 ttgcctgatg cccggcctgt gtcactttga ccgccagcgt ggcggctaag tcatcggctt
     2461 cgttgccgct ggttgaccag cacgggacgc cgcgttgctc aaaggcggcg cgtaatgcag
     2521 gcatctcgtc gtgcaactct tccggcattg gcggtcgacc cgctttgtaa tctggtaaac
     2581 gctgatgacg ccagccgcta ctgcggtttt catcgtcaaa aacggcgacc gcgtgggttg
     2641 gctggctgtg cataatgagc tgatcgagcg catgctggca ggtttcgaca cagggcgacc
     2701 cctgaacggc atgaatgcga cgaataagat tcagtgcatc gacaataagc aaatgaacag
     2761 ccacgataac ccccttaaag atcaggcgat aagggtaaca ttcgatactg gaacaggcta
     2821 tggctggatg agaaatcggg aagaggcctc gcaaaacgag gcctctggag agcgattaat
     2881 cgcaggcaac gatcttcatt gccaggccgc cgcgagaggt ttcgcggtac ttggcgttca
     2941 tatctttacc tgtttcgtac atggtttcga taactttatc gaggcagacg cgcggctcgc
     3001 tggtacggcg cagcgccata cgtgcggcgt tcaccgcttt tactgccgca atggcgttac
     3061 gctcgatgca tggcacctgt acctgtccgg cgaccgggtc acacgtcaga ccgaggttgt
     3121 gctccatggc gatttccgcc gcgatgcaca cctgcgccgg gcttgcacct aacagttctg
     3181 ccagaccagc cgccgccatt gagcacgcca caccaacttc accctggcaa cccacttcgg
     3241 caccagaaat cgacgcgttc atcttataaa gagaaccaat ggcgctggct accagcaggt
     3301 aacgagccag tgagttagcg ttcacttcgc ggataaactt atcgtagtac gccagcactg
     3361 ccgggataat cccgcacgca ccgttagtcg gcgcagtcac cacgcgaccg ccagcagcgt
     3421 tctcttcgtt cactgccagt gcaaacatgt tgatccagtc aacaaccgcc atcgggtcag
     3481 tggtggtttt atcctggctg accagcatcc ggcgtagtgc cgcagcacgg cgtggaacac
     3541 gcagtttgcc aggtaacaca ccttcggtgg aaataccgcg ctcaataccg ccacacatca
     3601 cttcccagac gttcgccagg tgctgttcca gctcttcttt gctgtgcagc gccagctcgt
     3661 ttttcatcat caggccagag agtgacagcc cggtttcctg acaatgtttt tgcagatcgg
     3721 ctgctgaact gtacggataa ggaacttcaa ccggtgcgct atcctgctgg ccaaaatgct
     3781 cttcatcaac gataaagcca ccgccaatag agtagtaagt ctgactgtaa acgactttat
     3841 cacccgccag cgcggtaatg cgcataccgt tttcatgcag agaaaggttg tcggcatgga
     3901 agttcatgca ctgatcaacc gggaactcca cttcatgctg accgtttgcc agcatcaggc
     3961 gaccatgagt attcacatcc tgaataaaac cagggatgga atcgatatcc acggtatccg
     4021 gcaggttacc cgccaggccc ataataatgg cgatatcagt gtggtggcct ttaccagtca
     4081 gagagagcga gccgtacacg tcaaccacca cgcgggtcac gtctttaagc aggttacggg
     4141 caatcagatc gtcggtaaat tgtttacccg ctttcattgg tccaacggta tgagaactgg
     4201 aagggccaat gccgattttg aaaatatcga atacgctaat cataggaaat acatcgcgtt
     4261 aaaacggagg aagcgccgcc cgaaagcggc gcgaaaggac ttagctgaac agagagtaga
     4321 agattgcgga gattgcaatc agacccatca cgacaacgaa tacgttgctg atgtgaccgc
     4381 tgtacttacg cattgccggt actttctgaa ttgcgtacat cggcatcagg aacaggatca
     4441 tcgcgatgat tgggccgccc agggtttcaa tcatacccag gatgctcggg ttcagggtgg
     4501 caacaatcca ggtcgttacc agcatgaaca gcgcagtgat acggttcagc ttgttgattt
     4561 cgatagactt acctttacca cgcagagatt taatcaccat accgttgaag ccttcacgtg
     4621 cgcccaggta gtggccgagg aaggatttgg tgatagcgat aatcgcgata atcggagcca
     4681 tccacgcgat aaccggtgcg ttaaagtggt tagccaggta agacagaatc gagatgttct
     4741 gctctttagc cgcagccagg tctgccggag tcaggctcag tacgcagctg aagacgaaga
     4801 acattacggt cagcaccatc atgatgtgtg cgaatgccag gatcttcgag catttctgtt
     4861 ctgccatatc gccgtactct tcacgcttcg caacggcgaa agaagagatg atcggagagt
     4921 ggttgaacga gaacaccatt accggaattg ccagccacag ggtcatccac agaccgtttc
     4981 cggttgcaga tgcagtgtcc agagacagcg tttccagtgc tgcgccgttc cactgcggga
     5041 tcaggtacag agccagcagc atcagtacgc caacaaacgg gaataccaga atactcatcg
     5101 ctttaacgat catctgttca ccaaagcgaa cgatggtcat catacccacg atcaggatca
     5161 gcgacagaat cgcacgcggc ggtggtgtca tacccagctg gtgagacatg aagctttcaa
     5221 ctgtattggt gattgccacg ctataaacca gcaggatcgg gtagatagcg aagaagtaga
     5281 gcagggtaat cagtttacct gcgccaatac caaagtgttc ttctacaacc tcggtaatgt
     5341 cttcgcccgg gtttttacca gacagtacga agcgagtcag gccgcggtga gcaaaaaacg
     5401 tcatcgggaa cgcaaggata gccatgatga tcagcgggat cataccgcca acaccggcgt
     5461 tgattggcag gaacagcacg cccgcgccga ttgccgtgcc gtaaaggccc agcatccaca
     5521 tggtgtctgt cttgcgccag gcactacgag agtctttcga cgcaatcgtg ctggtttgag
     5581 tcgtttccat ctatttctcc tggaggaatg caaataattc agcttttagc cagttcagcc
     5641 cgggaaggtg cctgaccgta aaaatgaaat ctgtctatcg gcgatggctt aatcatttac
     5701 cgccgtaata atttgagggc ggaaagatac acacaagtta tgtatctatc agtgatcttg
     5761 atcccaaata caataatcta cttggaagat agattatccc aacgctttac tctgtaaatc
     5821 aaaagtaaaa gagaatttaa gggcaatatt tgcccattaa acgacatctg acttcttcc
//
LOCUS       DAZKIF010000213         5815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZKIF010000213 DAZKIF010000000
VERSION     DAZKIF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.214"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            667..1926
                     /gene="codB"
                     /locus_tag="ACHOTW_004515"
     CDS             667..1926
                     /gene="codB"
                     /locus_tag="ACHOTW_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP9846481.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1916..3199
                     /gene="codA"
                     /locus_tag="ACHOTW_004516"
     CDS             1916..3199
                     /gene="codA"
                     /locus_tag="ACHOTW_004516"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP9846482.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3332..4231)
                     /gene="cynR"
                     /locus_tag="ACHOTW_004517"
     CDS             complement(3332..4231)
                     /gene="cynR"
                     /locus_tag="ACHOTW_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP9846483.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            4341..5000
                     /gene="cynT"
                     /locus_tag="ACHOTW_004518"
     CDS             4341..5000
                     /gene="cynT"
                     /locus_tag="ACHOTW_004518"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP9846484.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            5031..5501
                     /gene="cynS"
                     /locus_tag="ACHOTW_004519"
     CDS             5031..5501
                     /gene="cynS"
                     /locus_tag="ACHOTW_004519"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP9846485.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5534..>5815
                     /locus_tag="ACHOTW_004520"
     CDS             5534..>5815
                     /locus_tag="ACHOTW_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9846486.1"
                     /translation="MLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFSVAALLTALP
                     VVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLSS"
BASE COUNT         1248 a         1568 c         1593 g         1406 t
ORIGIN      
        1 tgcgccgatg cctgatgcga cgctgatgcg tcttatcatg cctacaggcc tgaaccgtag
       61 gtcggataag gcgctcgcgc cgcattcgac accatgcgcc gatgcctgat gcgacgcagg
      121 cgcgtcttat catgcctaca ggcctgaacc gtaggtcgga taaggcgctc gcgccgcatc
      181 cgacaccatg cgcagatgcc tgatgcgacg ctgatgcgtc ttatcatgcc tacaggcctg
      241 aaccgtaggt cggataaggc gctcgcaccg catccgacac cgtgctcagt gcctgatgcg
      301 acgctgatgc gtcttatcat gcctacaggc ctgaaccgta ggtcggataa ggcgctcgcg
      361 ccgcatccga caccgtgcgc agatgcctga tacgacgcag gcgcgtctta tcatgcctac
      421 aggcctgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaccgt gcgtagttgc
      481 ctgatgcgac gctgacgcgt cttatcatgc ctacccactg tttttacacc gataattttc
      541 cccccacctt tttgcactca ttcatataaa aaatatattt ccccacgaaa acgattgctt
      601 tttatcttca gatgaataga atgcggcgga ttttttgggt ttcaaacagc aaaaaggggg
      661 aatttcgtgt cgcaagataa caactttagc caggggccgg tcccgcagtc ggcgcggaaa
      721 ggggtattgg cattgacgtt cgtcatgctg ggattaacct tcttttccgc cagtatgtgg
      781 accggcggca ctctcggaac cggtcttagc tatcatgatt tcttcctcgc agttctcatc
      841 ggtaatcttc tcctcggtat ttacacttca tttctcggtt acattggcgc aaaaaccggc
      901 ctgaccactc atcttcttgc tcgcttctcg tttggtgtta aaggctcatg gctgccttca
      961 ctgctgctgg gcggcaccca ggttggctgg tttggcgtcg gtgtggcgat gtttgctatt
     1021 ccggtgggca aggcaaccgg gctggatatt aatttgctga ttgccgtttc cggtttactg
     1081 atgaccgtca ccgtcttctt tggcatttcg gcgctgacgg ttctttcgtt gattgcggtt
     1141 ccggctatcg cctgtcttgg cggttattcc gtgtggctgg ccgttaacgg catgggcggc
     1201 ctggacgcct taaaagcggt cgttcccgca caaccgttag atttcaatgt cgcgctggcg
     1261 ctggttgtgg ggtcatttat cagtgcgggc acgctcaccg ctgactttgt ccgctttggt
     1321 cgcaatgcca aactggcggt gctggtggcg atggtggcct ttttcctcgg caactcgttg
     1381 atgtttattt tcggtgcagc gggcgctgcg gcactgggga tggcggatat ctctgatgtg
     1441 atgattgctc agggcctgct gttgcctgcg attgtggtgc tggggctaaa tatctggacc
     1501 accaacgata acgcgctcta tgcgtcgggt ttaggtttcg ccaacattac cgggatgtcg
     1561 agcaaaaccc tttcggtaat caacggtatt atcggtacgg tctgtgcatt atggctgtat
     1621 aacaattttg tcggctggtt gaccttcctt tcggcagcta ttcctccagt gggtggcgtg
     1681 atcattgccg actatctgat gaaccgtcgc cgctatgagc actttgcgac cacgcgtatg
     1741 atgagtgtca attgggtggc gattctggcg gtcgccctgg ggattgctgc aggccactgg
     1801 ttaccgggaa ttgttccggt caacgcggta ttaggtggcg cgctgagcta tctgatcctt
     1861 aacccgattt tgaatcgtaa aacgacagca gcaatgacgc atgtggaggc taacagtgtc
     1921 gaataacgct ttacaaacaa ttattaacgc ccggttgcca ggcaaagagg ggctgtggca
     1981 gattcatctg caggacggaa aaatcagcgc cattgatgcg caatccggcg tgatgcccat
     2041 aactgaaaac agcctggatg ccgaacaagg tttagttata ccgccgtttg tggagccaca
     2101 tattcacctg gacaccacgc aaaccgccgg acaaccgaac tggaatcagt ccggcacgct
     2161 gtttgaaggc attgaacgct gggccgagcg caaagcgtta ttaacccatg acgatgtgaa
     2221 acaacgcgca tggcaaacgc tgaaatggca gattgccaac ggcattcagc atgtgcgtac
     2281 ccatgtcgat gtttcggatg caacgctaac tgcgctgaaa gcaatgctgg aagtgaagct
     2341 ggaagtcgcg ccgtggattg atctgcaaat cgtcgccttc cctcaggaag ggattttgtc
     2401 gtatcccaac ggtgaagcgt tgctggaaga ggcgttacgc ttaggggcag atgtagtggg
     2461 ggcgattccg cattttgaat ttacccgtga atacggcgtg gagtcgctgc ataaaacctt
     2521 cgccctggcg caaaaatacg accgtctcat cgacgttcac tgtgatgaga tcgatgacga
     2581 gcagtcgcgc tttgtcgaaa ccgttgctgc cctggcgcac cgtgaaggca tgggcgcgcg
     2641 agtcaccgcc agccacacca cggcaatgca ctcttataac ggggcgtata cctcacgtct
     2701 gttccgcttg ctgaaaatgt ccggtattaa ctttgtcgcc aacccgctgg tcaatattca
     2761 tctgcaagga cgattcgata cgtatccaaa acgtcgcggc atcacgcgcg ttaaagagat
     2821 gctggagtcc ggcattaacg tctgctttgg tcacgatgat gtcttcgatc cgtggtatcc
     2881 gctgggaacg gcgaatatgc tgcaagtgct gcatatgggg ctgcatgttt gccagctgat
     2941 gggctatggg cagattaacg atggcctgaa tttaatcacc caccacagcg ccaggacgtt
     3001 gaatttgcag gattacagca ttgccgccgg aaacagcgcc aacctgatta tcctgccggc
     3061 tgaaaatgga tttgatgcgc tgcgccgtca ggttccggta cgttattcgg tacgtggcgg
     3121 caaggtgatt gccagcacac aaccggcaca aaccaccgta tatctggagc agccggaagc
     3181 catcgattac aaacggtgat cggctgggct gtgtgttgac aaagcgagcg tagtttatgc
     3241 cggatgcggc ttgaacgcct tatcaggcct acgtagagca ctgaactcgt aggcctgata
     3301 agcgtaacgc atcaggcaat tgtgtcagtg gttaccgcga ttcattctcg ccaacatccg
     3361 cacattcctc caacgccatg tgcaaaaatg ccttcgcggc ggctgtctgc cagctatttt
     3421 tccgccgcaa caaaaccgcc gttctctcca gtagtggcgg ggcaagagga atcgctttaa
     3481 gcccgtcatg ttgtgtggca atcgctgctg gtaacaatgt ggaaagggaa gtgcggcgaa
     3541 tcagctccag aaccgcgcta attgagttcg cctcaatgac cacctgtgga tgcagccccg
     3601 ctttctcgca gtagtggtca atctgctctc tggtggcaaa ttccgcgctg agcaggacca
     3661 gtttttcatc atgcaagcga ctcaacgcca cctgttcaca ggcagccagc ggatgatgtt
     3721 gcgccacgac taacgctaaa ctttctgtca gtaaaggaat tgcctccagc tccggcaaat
     3781 gcacaggcgc gaaggcaatc ccaacgtcca gctcgtcgcg acaaagcaga tcttcgattt
     3841 tctcctgcga catttcctgt agctggagcg tgatgccggg gtagcgcgca tagaaatccg
     3901 ccattaaggg gccgataaag tagctcgtaa aggtgggggt gacggcgata cgcagcgatc
     3961 ctctcgtcag atcggcaaca tcatgaatcg cccgtttacc cgctcccagt tcctgtaacg
     4021 cccggctggc gtactgtcgc cagacttctc ctgcatcagt gagatgaatc gttcgcccgc
     4081 tacggtcaaa cagcggcacg cctaaactct cctctaactg gcgaatctgc tgggaaagcg
     4141 caggctgaga gacgtgcaac gcactggcgg cacgggtgaa gctgccatgt tcagccacgg
     4201 caagaaaata attgatatgt cgagagagca ttcgccaacc tataagtaaa ttcaatagaa
     4261 ctcatcataa atgagacttt taccttatga caatcggcga gtagtctgcc tctcattcca
     4321 gagacagata gaggttaacg gtgaaagaga ttattgatgg attccttaaa ttccagcgcg
     4381 aggcatttcc gaagcgggaa gccttattta aacagttggc gacacagcaa agcccgcgca
     4441 cactttttat ctcctgctcc gacagccgtc tggtccctga gctggtgacg caacgtgagc
     4501 ctggcgatct tttcgttatt cgcaatgcgg gcaatatcgt cccttcctac gggccggaac
     4561 ccggtggcgt ttctgcttcg gtggagtatg ccgtcgctgc gcttcgggta tctgacattg
     4621 tgatttgcgg tcattccaac tgtggcgcga tgaccgccat tgccagctgt cagtgcatgg
     4681 accatatgcc tgccgtctcc cactggctgc gttatgccga ttcagcccgc gtcgttaatg
     4741 aggcgcgccc gcattccgat ttaccgtcaa aagctgcggc gatggtacgt gaaaacgtca
     4801 ttgctcagtt ggctaatttg caaactcatc catcggtgcg cctggcgctc gaagaggggc
     4861 ggatcgccct gcacggctgg gtctacgaca ttgaaagcgg cagcatcgca gcgtttgacg
     4921 gcgcaacccg ccagtttgtg ccactggcag ctaatcctcg cgtttgtgcc ataccgctac
     4981 gccaaccgac cgcagcgtaa ccttattttt aaaccatcag gagatgcacc atgattcagt
     5041 cgcaaattaa ccgcaatatt cgtcttgatc ttgccgatgc cattttgctc agcaaagcta
     5101 aaaaagatct ctcatttgcc gagattgccg acggcaccgg gctggcagaa gcctttgtga
     5161 ccgcggcttt gctgggtcag caggcgcttc ctgccgacgc cgcccgccag gtcggggcga
     5221 agctggatct cgacgaagac gccattctac tgttacagat gatcccactg cgtggctgca
     5281 ttgatgaccg tattccaacc gacccaacga tgtatcgttt ctatgaaatg ttgcaggtgt
     5341 acggcacaac cctgaaagcg ttggttcatg agaaatttgg cgatggcatt atcagcgcga
     5401 ttaacttcaa actcgacgtt aagaaagtgg cggacccgga aggtggcgaa cgtgcggtca
     5461 tcaccttaga tggtaaatat ctgccgacca aaccgttctg acagccatgc gtaaccatca
     5521 aaagacgttc acgatgctgc tggttctggt gctgattggt cttaatatgc gaccactgct
     5581 cacctccgtc gggccactgc taccgcaatt gcgccaggca agcggaatga gctttagcgt
     5641 ggctgccctg ttgaccgctc tgccggtggt taccatgggc gggctggcgc tggccggaag
     5701 ctggcttcat cagcatgtca gcgaacgtcg cagcgtcgcc atcagtttgt tactgattgc
     5761 cgtcggtgca ttgatgcgtg agctttaccc tcaaagtgcg ctgctgctta gcagc
//
LOCUS       DAZKIF010000214         5777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZKIF010000214 DAZKIF010000000
VERSION     DAZKIF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.215"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2667
                     /locus_tag="ACHOTW_004521"
     CDS             <1..2667
                     /locus_tag="ACHOTW_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP9846487.1"
                     /translation="GTLNDFLGAMTEDDVRPEALRRFELMVEEVARNASAVAQNTAAA
                     KKSASDASTSAREAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEASK
                     SAAAAESSKSAAATSAGAAKTSETNAAVSQQSAATSASTATTKASEAASSARDASASK
                     EAAKSSETSAASSASSAASSATAAGNSAKAAKTSETNAKSSETAAEQSASAAAGSKTA
                     AALSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQASAAASSASAAK
                     TSETNAKASETSAESSKTAAASSASSAASSASSASASKDEATRQASAAKSSATTASTK
                     ATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSAT
                     NSTSESLAATPKAVKAAYELANGKYTAQDATTAQKGIVQLSNATNSTSEMLAATPKSV
                     KAAYDLANGKYTAQDATTAQKGIVQLSSATNSASETLAATPKAVKAANDNANGRVPSA
                     RKVNGKALSSDITLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITLIGGA
                     GFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAI
                     GNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIP
                     WPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQ
                     DGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTG
                     ASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVA
                     IGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            2667..3248
                     /locus_tag="ACHOTW_004522"
     CDS             2667..3248
                     /locus_tag="ACHOTW_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9846488.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     ASLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(3368..4258)
                     /locus_tag="ACHOTW_004523"
     CDS             complement(3368..4258)
                     /locus_tag="ACHOTW_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFP9846489.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(4277..4783)
                     /locus_tag="ACHOTW_004524"
     CDS             complement(4277..4783)
                     /locus_tag="ACHOTW_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9846490.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            complement(4820..5320)
                     /locus_tag="ACHOTW_004525"
     CDS             complement(4820..5320)
                     /locus_tag="ACHOTW_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9846491.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KAQDNS"
     gene            complement(5399..5581)
                     /locus_tag="ACHOTW_004526"
     CDS             complement(5399..5581)
                     /locus_tag="ACHOTW_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9846492.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
BASE COUNT         1555 a         1370 c         1389 g         1463 t
ORIGIN      
        1 ggtacgctga atgattttct tggtgccatg actgaggatg atgtccgtcc ggaggcactg
       61 cgccgttttg agctgatggt ggaagaggtg gcgcgtaacg cgtccgcggt ggcacagaac
      121 acggcagccg cgaagaagtc agccagcgat gccagcacat cagcccgtga ggcggcaacc
      181 catgcgactg atgctgcgga ctcagcacgc gcagccagca cgtcagccgg acaggccgcg
      241 tcgtcggctc agtcagcgtc ttccagcgca ggaacggcat caacaaaggc tactgaagca
      301 tcaaaaagtg ctgccgctgc agagtcctca aaaagcgcgg cggctaccag tgccggtgcg
      361 gcgaaaacgt cagaaacgaa tgcggcagtg tcacaacaat cagccgccac ttctgcatcc
      421 accgcgacca cgaaagcgtc agaagctgcc tcctcagcca gggatgcgtc ggcttcaaaa
      481 gaggcggcaa aatcatcaga aacgagcgca gcctcgagcg ccagtagtgc agcctcctcg
      541 gcaacggcgg caggcaattc cgcgaaggcg gccaaaacgt ctgagacaaa cgctaagtcc
      601 tctgaaacgg cagcagaaca gagtgcctcc gcagcagcag gctcaaaaac agcggctgca
      661 ttatctgcca gtgccgcgtc aacaagtgcc gggcaggcct cagccagtgc caccgccgcc
      721 ggaaaatcgg cagaaagtgc cgcatcgtct gcttcaacag ccacaacgaa ggctggcgaa
      781 gccactgaac aggccagcgc agcagcgagt tctgcttccg cagcgaagac atccgaaacg
      841 aacgcgaaag cgtcggaaac cagcgcagaa tcctcaaaaa cggctgccgc atcgtcagcc
      901 agttcggcgg cgtcatcggc atcatctgcg tctgcttcaa aagatgaggc gaccagacaa
      961 gcgtcagcag cgaagagcag cgccacgacg gcatccacga aggcgacaga ggcagctggt
     1021 agtgcgacgg cagcagctca gagcaaaagt acggcggaat ctgcagcaac gcgcgctgag
     1081 acagcggcaa aacgggcaga ggatattgca tccgccgtgg cgcttgagga tgcgagcacg
     1141 acgaaaaagg ggatagtaca gctcagcagt gcgactaaca gcacttccga gtcactggcg
     1201 gcaacgccaa aagccgttaa ggccgcgtat gagctggcta acgggaaata caccgcacag
     1261 gatgcaacga cagcacagaa agggatagtt cagcttagca acgcgaccaa cagcacatct
     1321 gaaatgctgg cggcaacgcc aaagtcggta aaggcagcct atgaccttgc taacgggaaa
     1381 tatactgctc aggacgctac gacagcacaa aaaggaattg tccagctcag tagtgcaacc
     1441 aacagcgcat ctgaaacgct tgccgcgaca ccgaaagcag tgaaagcagc taatgataat
     1501 gcgaatggtc gggtaccttc tgcccgtaag gtgaatggta aggcgctttc atcggatata
     1561 acactgacgc cgaaagatat tggtacgctt aactcaacaa caatgtcatt cagcggtggt
     1621 gctggttggt tcaaattagc aacggtaacc atgccacagg cgagttctgt tgtttcaatt
     1681 acgttgattg gtggcgcggg atttaacgtg gggtcacctc aacaggcagg tatatctgaa
     1741 cttgttttgc gtgcaggtaa tggtaatccg aaggggatta ctggtgcttt atggcagcgc
     1801 acatcgacag ggtttacaaa ttttgcctgg gtcaatacat ctggtgatac ttacgatatt
     1861 tacgttgcaa tcggaaatta tgcgactggt gtaaatattc aatgggatta taccagtaat
     1921 gccagcgtga cgattcatac gtcaccagca tattctgcta ataagccgga agggttaacg
     1981 gacggtacag tttattcact ctatacgcca tcagagcagt tttatccgcc tggcgcacca
     2041 atcccgtggc catcagatac cgttccgtct ggctatgccc tgatgcaggg gcagactttt
     2101 gacaaatctg catacccgaa acttgcagcc gcttatccgt caggcgtgat ccctgatatg
     2161 cgtggctgga cgattaaggg caaacctgcc agtggtcggg ccgtattgtc tcaggaacag
     2221 gacggcatta aatcgcacac ccacagcgcc agcgcatcca gtacggattt ggggacgaaa
     2281 accacatcgt cgtttgatta cggcactaaa tccacgaata acaccggggc gcacacgcac
     2341 agtgtgagcg gtacagccgc aagtgccgga aaccatactc atagtgtcac aggcgcatca
     2401 gcagtcagcc agtggtcaca aaatgggtca gtacataagg tagtgtctgc ggccagtgtg
     2461 aatacaagtg ctgcaggagc gcacactcat agtgtcagcg gcacagctgc atctgcaggt
     2521 gctcacgcac atactgtcgg tattggtgct catacgcact ctgttgcgat tggctcacat
     2581 ggacacacca tcaccgttaa cgctgcgggt aacgcggaaa acactgtcaa aaacatcgca
     2641 tttaactaca ttgtgaggct tgcataatgg cattcagaat gagtgaacaa ccacggacca
     2701 taaaaattta taatctgctg accggaacta atgaatttat tggtgaaggt gacgcatata
     2761 ttccgcctca tacaggtctg ccagcaaaca gtacctatat tgcaccgcca gatattccgg
     2821 ctggcttcgt ggctgttttc aacagtgatg agtcatcgtg gcatctcgtt gaagatcatc
     2881 ggggtaaaac ggtttatgac gtggcttccg gcgacgcgtt atttatttct gaactcggct
     2941 cattaccgga aaatgttacc tggttatcgc cggaagggga gtttcagaag tggaacggca
     3001 cagcctgggt gaaagatgca gaagcagaaa aactgttccg gatccgggag gcggaagaaa
     3061 caaaaaacag cctgatgcag gtagccagtg agcatattgc gccacttcag gatgctgtag
     3121 atctggaaat cgcaacggag gaagaagcct cattgctgga agcctggaaa aagtatcggg
     3181 tgttgctgaa ccgtgttgat acatcaactg cacctgatat tgagtggcct gtaatacctg
     3241 aagtttaaaa taaaagcccg cttaaaatat cgcgggcttc gaaatataaa tgtattttca
     3301 gaggctagag cttaggatat ctcagcggca attaacttat gagggatagc taaataccag
     3361 aagataatta gtttttacta tttatctttg tttgtggttc tccttcagca agctcagcgc
     3421 cagtgacagg attgatgtct tcatgggatt tcaaccttgt tgccatagct tttatcatag
     3481 cttcttgctt ggtcgaaaga tttatcgagg ccttcccatc cccaccgtca accgctgaaa
     3541 ctggatcgga aacatagtca aagttttcat cactgttcca actacctctg acgtcttcac
     3601 cttctgacat gttgtaatat acgtttttgt atttctcaag tggtggtaat tttcctggtg
     3661 gaaaggtgtt acgaattgaa tgtaatgctt tttcaaatga aagcatgtgt gctgcttccc
     3721 gggtcattaa aaatgccaga gtgtctttta ctccaggatc atcagtaaca ttaatgagac
     3781 gttcgtaaat gatctttgcc cgagcttcag ctgcaatatt tgaacgaaga tcggccgtga
     3841 cttcgccaat agtatcaaca taagctgcag tccagggtac tccagctgaa ttagttaatg
     3901 ctgggcctcc tccgtagagg agagaagtta tatggctatc attgccattt tgagttaagg
     3961 aacggtaaag ctcagcttca ttctcagttc cttcagcaag tgctcctttt gcacctttgt
     4021 tgagcatacc aacaagagaa ccaataattt cgagatgact tagttcttct gttgctatgt
     4081 ccatcagcat atcccttcgg cctgcatctt catcacttaa gccttgagtg aagtatcggc
     4141 atgctgctgc aagctcaccc tgtggcccac cgaattgttc taagagtaga ttagccaagc
     4201 ctgggtttgg ctcacttaca cgtactgtat attgaagttt tttcacgtgt ctaaacataa
     4261 tgcctcctca aaagaattat tttttggctt caactccatc aatttcagat ctgaccatga
     4321 attgctcagt ggtgtcagga atatggttaa gaagccattc agccatttgt ttttcttcat
     4381 taagaattgc ttcaataatg ggtactgaag ctgtatcacc cgcatttttg gctgctgcga
     4441 gaagagatgt gtaacacgca atttcaaatt gttcgaacac atagccactg atcgaacctt
     4501 tgacaatttc atcagatgga aaaattccac caatggattg tccaaacgcg gccattttac
     4561 tcatagagtc tttaatgaca gagcgcgaaa tattattacg atcaagtatg ctttcaagct
     4621 gtgaaagctg gttctttgtt tcgctaatgt gctgctcaat tctggaacgt aactcaggat
     4681 agttttctat ccggctggcc atagactcaa gcattttttc agcttgtttt tccatcgcat
     4741 gtgcatcacg aagccagtca tggtaatgtt caattctatt cataatctgt ttatcctaaa
     4801 agaaattgaa gtctatagtt caggagttat cctgagcttt tttattaacg ttattaaatg
     4861 ccaggtctgt taatttgaca tcagtagctt tttcctcttc cagtgtttct ttcaggagtt
     4921 tagccgcttt tttatatcct aattgctcag cgagtgttac caaagtgcca taactggcaa
     4981 tttcatagtg ctctactttt tgtgctgcag caatcaatgc agcatcacgt acttcatttt
     5041 tatcagtgct ttcaattacc tcattcgcct cttcgataag tccttccatt gcggcacact
     5101 taattctttt caatttaaga ccatcttcag aatcaacaac ctgatcgatg cgttcaattt
     5161 gaccatgtgt ctcatcaaga tgtgactgaa aagcagcggt taatttatca ctatatgcag
     5221 agcgactaag tttagacagt gcctttgtta gttgtttctc agcgctgtat gtatccgata
     5281 ataagtggat gaagatatct tcaacagatt taatttgcat aatcactcca gagttttcac
     5341 tgcgagcccg taggctcgca gttagcttca gttattcacg aatgtagggg gtgataaatt
     5401 aagagtcaga tttacggccg ccaccatggc tattctgacc accttttttc cctgcttctg
     5461 atgctctttg tggatcgttt ttaaaattac cgccactatg ttggccgcct ttacgaccag
     5521 cgtcagaagc tttttgtcga tcttcagcga aattacctgc accgccacga tgttcggtca
     5581 tatcatttct cctctgattt tcaaattgta aataacatgt gccttgtcag cacccaatta
     5641 actctagtaa caaataagaa agtgtcaaat aagagattgg tatgcgcatc ttaatgtgag
     5701 aaatagcaat tgtaaaaatg tgtactcagt gagtataatc tgttgatatc ttggtgatta
     5761 acggaaagtt taggttg
//
LOCUS       DAZKIF010000215         5762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZKIF010000215 DAZKIF010000000
VERSION     DAZKIF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.216"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            74..964
                     /gene="prpB"
                     /locus_tag="ACHOTW_004527"
     CDS             74..964
                     /gene="prpB"
                     /locus_tag="ACHOTW_004527"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP9846493.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            1120..2289
                     /gene="prpC"
                     /locus_tag="ACHOTW_004528"
     CDS             1120..2289
                     /gene="prpC"
                     /locus_tag="ACHOTW_004528"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP9846494.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            2323..3774
                     /gene="prpD"
                     /locus_tag="ACHOTW_004529"
     CDS             2323..3774
                     /gene="prpD"
                     /locus_tag="ACHOTW_004529"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP9846495.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            3814..5700
                     /gene="prpE"
                     /locus_tag="ACHOTW_004530"
     CDS             3814..5700
                     /gene="prpE"
                     /locus_tag="ACHOTW_004530"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP9846496.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
BASE COUNT         1282 a         1571 c         1681 g         1228 t
ORIGIN      
        1 taaggcaatt gtggcacacc ccttgctttg tcgttatcaa cgaaaataac aagttgataa
       61 caaaggatgg gctatgtctc tacactctcc aggtaaagcg tttcgcgctg cactgactaa
      121 agaaaatcca ttgcagattg ttggcaccat taacgccaac catgcgctgt tggcgcagcg
      181 tgccggatat caggcaattt atctctccgg cggtggcgtg gcggcagggt cgctggggct
      241 gcccgatctc ggtatttcta ctcttgatga cgtgttgaca gatattcgcc gtatcaccga
      301 cgtttgttcg ctgccgctgc tggtggatgc ggatatcggt tttggttctt ctgcttttaa
      361 cgttgcgcgt acggtgaaat cgatgattaa agccggtgcg gcgggattgc atattgaaga
      421 tcaggttggt gcaaaacgct gtggtcatcg cccgaacaaa gcgatcgtct cgaaagagga
      481 gatggtggat cggatccgcg cggcggtgga tgcgaaaacc gatcctgatt ttgtgatcat
      541 ggcgcgcacc gatgcgctgg cggtagaggg gctggatgcg gcgatcgagc gtgcgcaggc
      601 ctatgttgaa gcgggtgccg agatgttgtt cccggaggcg attaccgaac tcgccatgta
      661 ccgccagttt gccgatgcgg tgcaggtgcc aatcctcgcc aacattaccg aatttggcgc
      721 cacgccgctg tttaccaccg acgaattacg cagcgctcat gtcgcgatgg cgctgtaccc
      781 gctttcagcg tttcgcgcca tgaaccgcgc cgccgaacat gtctacaacg tcctgcgcca
      841 ggaaggcacg cagaaaagcg tcatcgacac catgcagacc cgcaacgagc tgtacgaaag
      901 catcaactac taccactacg aagagaagct cgacgacctg tttgtccgta gccaggcgaa
      961 ataaaaaacg cccgttggtt gtattcgaca accgatgcct gatgcgccgc tgacgcgact
     1021 tatcaggcct acggggtgaa ctgtaggtcg gataagacgc atggcgtcgc atccgacaac
     1081 aatctcgacc ctacaaatga taacaatgac gaggacaata tgagcgacac aacgatcctg
     1141 caaaacagta cccatgtcat taaaccgaaa aaatctgtgg cgctttctgg cgttccggcg
     1201 ggcaatacgg cgctctgcac cgtgggtaaa agtggcaatg acttgcatta ccgtggctac
     1261 gatattcttg atctggcgga acattgcgaa tttgaagaag tggcgcatct gctgatccac
     1321 ggcaaactgc cgacccgtga cgaactcgcc gcctacaaaa cgaaactgaa agccctgcgc
     1381 ggtttaccgg ctaacgtgcg taccgtgctg gaagccttac cggcggcgtc acacccgatg
     1441 gatgttatgc gcaccggcgt ttccgcgctc ggctgcacgc tgccagaaaa agaggggcat
     1501 accgtctctg gcgcgcggga tattgccgac aaactgctgg cgtcacttag ctcgattctt
     1561 ctctactggt atcactacag ccacaacggc gaacgcatcc agccggaaac cgatgacgac
     1621 tccatcggtg gtcacttcct gcatctgctg cacggcgaaa agccgtcgca aagctgggaa
     1681 aaggcgatgc atatttcgct ggtgctgtac gccgaacacg agtttaacgc ctccaccttt
     1741 accagccggg tgattgcggg cactggctct gatatgtatt ccgcgattat tggcgcgatt
     1801 ggtgcactgc gcgggccgaa gcacggcggg gcgaatgaag tgtcgctgga gatccagcaa
     1861 cgctacgaaa cgccggacga agccgaagcc gatatccgca agcgggtgga aagcaaagaa
     1921 gtggtcattg gttttggtca tccggtttat accatcgccg acccgcgcca ccaggtgatt
     1981 aaacgtgtgg cgaagcagct ctcgcaggaa ggcggctcgc tgaagatgta caacatcgcc
     2041 gatcgcctgg aaacggtgat gtgggagagc aaaaagatgt tccccaatct cgactggttc
     2101 tccgctgttt cctacaacat gatgggcgtt cccaccgaga tgttcacacc actgtttgtt
     2161 atcgcccgcg tcaccggctg ggcggcgcac attatcgaac aacgtcagga caacaaaatt
     2221 atccgtcctt ccgccaatta tgttggaccg gaagaccgcc agtttgtcgc gctggataag
     2281 cgccagtaaa cctctacgaa taacaataag gaaacgtacc caatgtcagc tcaaatcaac
     2341 aacatccgcc cggaatttga tcgtgaaatc gttgatatcg tcgattacgt catgaactac
     2401 gaaatcagct ccaaagtagc ctacgacacc gcacattact gtctgctcga cacgctcggc
     2461 tgcggtcttg aagctctcga atacccagcc tgtaaaaaac tgctggggcc aattgtcccc
     2521 ggcaccgtcg tacctaacgg cgtgcgcgtc cccggaactc agttccagct cgaccccgtc
     2581 caggcggcat ttaacatcgg cgcgatgatc cgctggctcg atttcaacga tacctggctt
     2641 gcggcagagt ggggccatcc ttccgacaat ctcggcggca ttctggcaac ggcggactgg
     2701 ctttcacgca acgcggtcgc cagcggcaaa gcgccgttga ccatgaaaca ggtgctgacc
     2761 ggaatgatta aagcccatga aattcagggc tgcatcgcgc tggaaaactc ctttaaccgc
     2821 gtcggcctcg accacgttct gttagtgaaa gtggcttcca ccgccgtggt cgccgaaatg
     2881 cttggcctga cccgcgagga aattctcaac gctgtttcgc tggcgtgggt ggacggtcag
     2941 tcgctgcgca cctatcgcca tgcgccgaac accggcacgc gtaaatcctg ggcggcgggc
     3001 gatgccactt cccgcgcggt acgtctggca ctgatggcga aaacgggcga aatgggttac
     3061 ccgtcagccc tgactgcgcc tgtgtggggc ttctacgacg tctcctttaa aggtgaatcg
     3121 ttccgcttcc agcgtccgta cggttcttac gtgatggaaa atgtgctgtt caaaatctcc
     3181 ttcccggcgg agttccactc ccagacggca gttgaagcag cgatgacgct ctatgaacag
     3241 atgcaggcag caggcaaaac ggcggcagat atcgaaaaag tgtccatccg cacccacgaa
     3301 gcctgtattc gcatcatcga caaaaagggg ccgctcaata acccggcaga ccgcgaccac
     3361 tgcattcagt acatggtggc gatcccactg ctattcgggc gcttaacggc ggcagattac
     3421 gaggacaacg ttgcgcaaga taaacgcatt gacgccctgc gcgagaagat caattgcttt
     3481 gaagatccgg tatttaccgc tgactaccac gacccggaaa aacgcgccat cgccaatgcc
     3541 attacccttg agttcaccga cggcacacga tttgaagaag tggtggtgga gtaccccatt
     3601 ggtcatgctc gccgccgtca ggatggtatt ccgaaactgg tcgataaatt caaaatcaat
     3661 ctcgcgcgcc agttcccgac tcgccaacag cagcgcattc tggaggtttc tctcgacaga
     3721 gctcgcctgg aacagatgcc ggtcaatgag tatctcgacc tgtacgtcat ttaagtaaac
     3781 ggcggtaagg cgtaagttca acaggagagc attatgtctt ttagcgaatt ttatcagcgt
     3841 tcgattaacg aaccggagca gttctgggcc gagcaggccc ggcgtattga ctggcagacg
     3901 ccctttacgc aaacgctcga tcacagcaat ccgccgtttg cccgttggtt ttgtgaaggc
     3961 cgaaccaact tgtgccacaa cgctatcgac cgctggctgg agaaacaacc agaggcgctg
     4021 gcgctgattg ccgtctcttc ggaaacagaa gaagagcgca cctttacctt tcgccagctt
     4081 catgacgaag taaacgcggt ggcgtcaatg ctgcgctcgc tgggcgtgca gcgtggcgat
     4141 cgggtgctgg tgtatatgcc gatgattgcc gaagcgcata ttactctgct ggcctgcgcg
     4201 cgcattggcg ctattcactc ggtggtgttt gggggatttg cctcgcacag cgtggcggcg
     4261 cgaattgatg atgctaaacc ggtgctgatt gtctcggctg atgccggagc gcgcggtggc
     4321 aaaatcattc cctataaaaa attgctcgac gatgcgataa gtcaggcgca gcaccagcca
     4381 cgccatgttt tgctggtgga tcgcgggctg gcgaaaatgg cacgcgtcag cgggcgggat
     4441 gtcgatttcg cgtcgttgcg ccatcaacac atcggcgcgc gggtaccggt ggcgtggctg
     4501 gaatccaacg aaacctcctg cattctctac acctccggca cgaccggcaa acctaaaggc
     4561 gtgcagcgtg acgttggcgg atatgcggtg gcgctggcga cctcgatgga caccattttt
     4621 ggcggcaaag cgggcggtgt gttcttttgt gcttcggata tcggctgggt ggtggggcat
     4681 tcgtatatcg tctacgcgcc gctgctggcg gggatggcga ctatcgttta cgaaggatta
     4741 ccgacctggc cggactgcgg cgtgtggtgg aaaatcgtcg agaaatatca ggttagccgg
     4801 atgttctcag cgccgaccgc cattcgcgtg ctgaaaaaat tccctaccgc tgaaattcgc
     4861 aaacacgatc tctcgtcgct ggaagtgctc tatctggctg gagaaccgct ggacgagccg
     4921 accgccagtt gggtgagcaa tacgctggat gtgccggtca tcgacaacta ctggcagacc
     4981 gaatccggct ggccgattat ggcgattgct cgcggtctgg acgacaggcc gacgcgtctg
     5041 ggaagccccg gcgtgccgat gtatggctat aacgtgcagt tgcttaatga agtcaccggc
     5101 gaaccgtgtg gcgtcaacga gaaagggatg ctggtggtgg aagggccgct gccgccgggg
     5161 tgtattcaga ccatctgggg cgacgacgac cgctttgtga agacgtactg gtcgctgttt
     5221 tcccgcccgg tgtacgccac ctttgactgg ggcatccgtg acgctgacgg ttatcacttt
     5281 attctcgggc gcactgacga tgtgattaac gttgccgggc atcggctggg gacgcgcgag
     5341 attgaagaga gtatctccag tcatccgggc gttgccgaag tggcggtggt tggggtgaaa
     5401 gatgcgctga aagggcaggt ggcggtggcg tttgtcattc cgaaagagag cgacagtctg
     5461 gaagatcgtg atgtggcgca ttcgcaagag aaggcgatta tggcgctggt ggacagccag
     5521 attggcaact ttggccgccc ggcgcacgtc tggtttgtct cgcaattgcc aaaaacgcga
     5581 tccggaaaaa tgctgcgccg cacgatccag gcgatttgcg aaggacgcga tcctggggat
     5641 ctgacgacca ttgatgatcc tgcgtcgttg aatcagatcc gccaggcgat ggaagagtaa
     5701 ggtattgtcg gatgcgtcgc gtggcgcatc cgacagttgt gcgcagatgc ctgatgcgac
     5761 gc
//
LOCUS       DAZKIF010000216         5695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZKIF010000216 DAZKIF010000000
VERSION     DAZKIF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.217"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..76
                     /locus_tag="ACHOTW_004531"
     CDS             <1..76
                     /locus_tag="ACHOTW_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846497.1"
                     /translation="GLKRRNACADFLLDVVKQEIESRS"
     gene            96..1214
                     /gene="gmd"
                     /locus_tag="ACHOTW_004532"
     CDS             96..1214
                     /gene="gmd"
                     /locus_tag="ACHOTW_004532"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9846498.1"
                     /translation="MTKVALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERI
                     DHIYQDPHGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSF
                     VGKGIEEKGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWR
                     PEITLAEMISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
     gene            1217..2182
                     /locus_tag="ACHOTW_004533"
     CDS             1217..2182
                     /locus_tag="ACHOTW_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFP9846499.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMA
                     SASIYVMEMPYDIWQKNTKVMLSHINIGTGIDCTICELAETIAKVVGYKGHITFDTTK
                     PDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYRG"
     gene            2185..2694
                     /locus_tag="ACHOTW_004534"
     CDS             2185..2694
                     /locus_tag="ACHOTW_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP9846500.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
                     KDIICLMRQ"
     gene            2676..4124
                     /locus_tag="ACHOTW_004535"
     CDS             2676..4124
                     /locus_tag="ACHOTW_004535"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFP9846501.1"
                     /translation="MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTT
                     LLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHA
                     LKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
                     IERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLE
                     ELRKFRPDIYNVCEQVASSSYIDLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPV
                     DIGWSDVGSWQSLWDISLKSKTGDVCKGDILTYDTKNNYIYSESALVAAIGIEDMVIV
                     QTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKK
                     IIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENP
                     GIIPLNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            4128..5498
                     /gene="cpsG"
                     /locus_tag="ACHOTW_004536"
                     /gene_synonym="manB"
     CDS             4128..5498
                     /gene="cpsG"
                     /locus_tag="ACHOTW_004536"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP9846502.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKAL
                     LKLLSE"
BASE COUNT         1684 a         1094 c         1285 g         1632 t
ORIGIN      
        1 gggtctcaaa agacgcaatg cgtgtgcaga ttttctttta gatgttgtga aacaagaaat
       61 tgaatcccga tcttaatata ttcaagaggt atataatgac taaagtcgct cttattacag
      121 gtgtaactgg acaagatgga tcttatctag ctgagttttt gcttgataaa gggtatgaag
      181 ttcatggtat caaacgccga gcctcatctt ttaatacaga acgcatagac catatttatc
      241 aagatccaca tggttctaac ccaaattttc acttgcacta tggagatctg actgattcat
      301 ctaacctcac tagaattcta aaggaggtac agccagatga agtatataat ttagctgcta
      361 tgagtcacgt agcagtttct tttgagtctc cagaatatac agccgatgtc gatgcaattg
      421 gtacattacg tttactggaa gcaattcgct ttttaggatt ggaaaacaaa acgcgtttct
      481 atcaagcttc aacctcagaa ttatatggac ttgttcagga aatccctcaa aaagaatcca
      541 ccccttttta tcctcgttcc ccttatgcag ttgcaaaact ttacgcatat tggatcacgg
      601 taaattatcg agagtcatat ggtatttatg catgtaatgg tatattgttc aatcatgaat
      661 ctccacgccg tggagaaacg tttgtaacaa ggaaaattac tcgaggactt gcaaatattg
      721 cacaaggctt ggaatcatgt ttgtatttag ggaatatgga ttcgttacga gattggggac
      781 atgcaaaaga ttatgttaga atgcaatggt tgatgttaca acaggagcaa cccgaagatt
      841 ttgtgattgc aacaggagtc caatactcag tccgtcagtt tgtcgaaatg gcagcagcac
      901 aacttggtat taagatgagc tttgttggta aaggaatcga agaaaaaggc attgtagatt
      961 cggttgaagg acaggatgct ccaggtgtga aaccaggtga tgtcattgtt gctgttgatc
     1021 ctcgttattt ccgaccagct gaagttgata ctttgcttgg agatccgagc aaagctaatc
     1081 tcaaacttgg ttggagacca gaaattactc ttgctgaaat gatttctgaa atggttgcca
     1141 aagatcttga agccgctaaa aaacattctc ttttaaaatc gcatggtttt tctgtaagct
     1201 tagctctgga atgatgatga ataagcaacg tatttttatt gctggtcacc aaggaatggt
     1261 tggatcagct attacccgac gcctcaaaca acgtgatgat gttgagttgg ttttacgtac
     1321 tcgggatgaa ttgaacttgt tggatagtag cgctgttttg gatttttttt cttcacagaa
     1381 aatcgaccag gtttatttgg cagcagcaaa agtcggaggt attttagcta acagttctta
     1441 tcctgccgat tttatatatg agaatataat gatagaggcg aatgtcattc atgctgccca
     1501 caaaaataat gtaaataaac tgcttttcct cggttcgtcg tgtatttatc ctaagttagc
     1561 acaccaaccg attatggaag acgaattatt acaagggaaa cttgagccaa caaatgaacc
     1621 ttatgctatc gcaaaaattg caggtattaa attatgtgaa tcttataacc gtcagtttgg
     1681 gcgtgattac cgttcagtaa tgccaaccaa tctttatggt ccaaatgaca attttcatcc
     1741 aagtaattct catgtgattc cggcgctttt gcgccgcttt catgatgctg tggaaaacaa
     1801 ttctccgaat gttgttgttt ggggaagtgg tactccaaag cgtgaattct tacatgtaga
     1861 tgatatggct tctgcaagca tttatgtcat ggagatgcca tacgatatat ggcaaaaaaa
     1921 tactaaagta atgttgtctc atatcaatat tggaacaggt attgactgca cgatttgtga
     1981 gcttgcggaa acaatagcaa aagttgtagg ttataaaggg catattacgt tcgatacaac
     2041 aaagcccgat ggagcccctc gaaaactact tgatgtaacg cttcttcatc aactaggttg
     2101 gaatcataaa attacccttc acaagggtct tgaaaataca tacaactggt ttcttgaaaa
     2161 ccaacttcaa tatcgggggt aataatgttt ttacattccc aagactttgc cacaattgta
     2221 aggtctactc ctcttatttc tatagatttg attgtggaaa acgagtttgg cgaaattttg
     2281 ctaggaaaac gaatcaaccg cccggcacag ggctattggt tcgttcctgg tggtagggtg
     2341 ttgaaagatg aaaaattgca gacagccttt gaacgattga cagaaattga actaggaatt
     2401 cgtttgcctc tctctgtggg taagttttat ggtatctggc agcacttcta cgaagacaat
     2461 agtatggggg gagacttttc aacgcattat atagttatag cattccttct taaattacaa
     2521 ccaaacattt tgaaattacc gaagtcacaa cataatgctt attgctggct atcgcgagca
     2581 aagctgataa atgatgacga tgtgcattat aattgtcgcg catattttaa caataaaaca
     2641 aatgatgcga ttggcttaga taataaggat ataatatgtc tgatgcgcca ataattgctg
     2701 tagttatggc cggtggtaca ggcagtcgtc tttggccact ttctcgtgaa ctatatccaa
     2761 agcagttttt acaactctct ggtgataaca ccttgttaca aacgactttg ctacgacttt
     2821 caggcctatc atgtcaaaaa ccattagtga taacaaatga acagcatcgc tttgttgtgg
     2881 ctgaacagtt aagggaaata aataaattaa atggtaatat tattctagaa ccatgcgggc
     2941 gaaatactgc accagcaata gcgatatctg cgtttcatgc gttaaaacgt aatcctcagg
     3001 aagatccatt gcttctagtt cttgcggcag accacgttat agctaaagaa agtgttttct
     3061 gtgatgctat taaaaatgca actcccatcg ctaatcaagg taaaattgta acgtttggaa
     3121 ttataccaga atatgctgaa actggttatg ggtatattga gagaggtgaa ctatctgtac
     3181 cgcttcaagg gcatgaaaat actggttttt attatgtaaa taagtttgtc gaaaagccta
     3241 atcgtgaaac cgcagaattg tatatgactt ctggtaatca ctattggaat agtggaatat
     3301 tcatgtttaa ggcatctgtt tatcttgagg aattgagaaa atttagacct gacatttaca
     3361 atgtttgtga acaggttgcc tcatcctcat acattgatct agattttatt cgattatcaa
     3421 aagaacaatt tcaagattgt cctgctgaat ctattgattt tgctgtaatg gaaaaaacag
     3481 aaaaatgtgt tgtatgccct gttgatattg gttggagtga cgttggatct tggcaatcgt
     3541 tatgggacat tagtctaaaa tcgaaaacag gagatgtatg taaaggtgat atattaacct
     3601 atgatactaa gaataattat atctactctg agtcagcgtt ggtagccgcc attggaattg
     3661 aagatatggt tatcgtgcaa actaaagatg ccgttcttgt gtctaaaaag agtgatgtac
     3721 agcatgtaaa aaaaatagtc gaaatgctta aattgcagca acgtacagag tatattagtc
     3781 atcgtgaagt tttccgacca tggggaaaat ttgattcgat tgaccaaggt gagcgataca
     3841 aagtcaagaa aattattgtg aaacctggtg aggggctttc tttaaggatg catcaccatc
     3901 gttctgaaca ttggatcgtg ctttctggta cagcaaaagt aacccttggc gataaaacta
     3961 aactagtcac cgcaaatgaa tcgatataca ttccccttgg cgcagcgtat agtcttgaga
     4021 atccgggcat aatccctctt aatcttattg aagtcagttc aggggattat ttgggagagg
     4081 atgatattat aagacagaaa gaacgttaca aacatgaaga ttaacatatg aaatctttaa
     4141 cctgctttaa agcctatgat attcgcggga aattaggcga agaactgaat gaagatattg
     4201 cctggcgcat tgggcgtgcc tatggcgaat ttctcaaacc gaaaaccatt gttttaggcg
     4261 gtgatgtccg cctcaccagc gaagcgttaa aactggcgct tgcgaaaggt ttacaggatg
     4321 cgggcgtcga tgtgctggat atcggtatgt ccggcaccga agagatctat ttcgccacgt
     4381 tccatctcgg agtggatggc ggcatcgaag ttaccgccag ccataacccg atggattaca
     4441 acggcatgaa gctggtgcgc gaaggggctc gcccgatcag cggtgatacc ggactgcgcg
     4501 atgtccagcg tctggcagaa gccaatgact tccctcctgt cgatgaaacc aaacgtggtc
     4561 gctatcagca aatcaatctg cgtgacgctt acgttgatca cctgttcggt tatatcaacg
     4621 tcaaaaacct cacgccgctc aagctggtga tcaactccgg gaacggcgca gcgggtccgg
     4681 tggtggacgc cattgaagcc cgatttaaag ccctcggcgc accggtggaa ttaatcaaag
     4741 tacacaacac gccggacggc aatttcccca acggtattcc taacccgctg ctgccggaat
     4801 gccgcgacga cacccgtaat gcggtcatca aacacggcgc ggatatgggc attgcctttg
     4861 atggcgattt tgaccgctgt ttcctgtttg acgaaaaagg gcagtttatc gagggctact
     4921 acattgtcgg cctgctggca gaagcgttcc tcgaaaaaaa tcccggcgcg aagatcatcc
     4981 acgatccacg tctctcctgg aacaccgttg atgtggtgac tgccgcaggc ggcaccccgg
     5041 taatgtcgaa aaccggacac gcctttatta aagaacgtat gcgcaaggaa gacgccatct
     5101 acggtggcga aatgagcgct caccattact tccgtgattt cgcttactgc gacagcggca
     5161 tgatcccgtg gctgctggtc gccgaactgg tgtgcctgaa aggaaaaacg ctgggcgaaa
     5221 tggtgcgcga ccggatggcg gcgtttccgg caagcggtga gatcaacagc aaactggcgc
     5281 aacccgttga ggcaattaat cgcgtggaac agcattttag ccgcgaggcg ctggcggtgg
     5341 atcgcaccga tggcatcagc atgacctttg ccgactggcg ctttaacctg cgctcctcca
     5401 acaccgaacc ggtggtgcgg ttgaatgtgg aatcacgcgg tgatgtaaag ctaatggaaa
     5461 agaaaactaa agctcttctt aaattgctaa gtgagtgatt atttacatta atcattaagc
     5521 gtatttaaga ttatattaaa gtaatgttat tgcggtatat gatgaatatg tgggcttttt
     5581 tatgtataac gactataccg caactttatc taggaaaaga ttaatagaaa taaagttttg
     5641 tactgaccaa tttgcatttc acgtcacgat tgagacgttc ctttgcttaa gacat
//
LOCUS       DAZKIF010000217         5369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZKIF010000217 DAZKIF010000000
VERSION     DAZKIF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.219"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHOTW_004537"
     CDS             complement(<1..209)
                     /locus_tag="ACHOTW_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9846503.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLRD"
     gene            1033..1806
                     /locus_tag="ACHOTW_004538"
     CDS             1033..1806
                     /locus_tag="ACHOTW_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846504.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAAADDDDLLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASKAEVILATVRGIVRGVFIADKWLKSTREHFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(1864..2418)
                     /gene="pinE"
                     /locus_tag="ACHOTW_004539"
     CDS             complement(1864..2418)
                     /gene="pinE"
                     /locus_tag="ACHOTW_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HFP9846505.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIP
                     RQKVAIIYDVGVSTLYKKFPAGDK"
     gene            2448..2858
                     /locus_tag="ACHOTW_004540"
     CDS             2448..2858
                     /locus_tag="ACHOTW_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9846506.1"
                     /translation="MPFARYFCIFINVGLGEAAKRDVGTGENQIPDMASFASGNGWMK
                     LPNGKILQYGRGAVTPTLSTQTMRITFSIPFPKKADCAMLTHSGDGGAPLGAGRGFVM
                     TAEGPTLTGFNSAYRTSSTSDTVSMNYSWWAVGE"
     gene            2879..3322
                     /locus_tag="ACHOTW_004541"
     CDS             2879..3322
                     /locus_tag="ACHOTW_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFP9846507.1"
                     /translation="MDEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVNQNIISEFM
                     AEPPQGKIRIAGDDGLPAWADIPPPTHEELIEITESERQLLINQANEYMNSKQWPGKA
                     AIGRLKGDELTQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
     gene            complement(3294..3887)
                     /locus_tag="ACHOTW_004542"
     CDS             complement(3294..3887)
                     /locus_tag="ACHOTW_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9846508.1"
                     /translation="MKPVFDENGLATVPGDMRCFYYDAETSEYTGWSDEYINTGVSMP
                     ACSTGIDPGENIPGRVAVFTGKGWSHEEDHRNETVYSIENGAAVTVDYIGAIKDGYVT
                     ISPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAGRKL
                     TQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(3887..4681)
                     /locus_tag="ACHOTW_004543"
     CDS             complement(3887..4681)
                     /locus_tag="ACHOTW_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9846509.1"
                     /translation="MAKNDFKAFATDRNANVMSQEEWEALPALISGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTALQNLGLGEGAPAIGVPFFWPSAAMPNTVIDSWSCMVFLKFNGAKFSATDYP
                     VLAKVFPSLVLPEARGDFIRIWDDGRGADGGRELLSWQAATNFSQFAGNIGEGAGHAI
                     NFHDGIAGNQPGFSRFNFTSNSVGDGVNFVAVRPRNIAFNFLVRAK"
     gene            complement(4681..4929)
                     /locus_tag="ACHOTW_004544"
                     /pseudo
     CDS             complement(4681..4929)
                     /locus_tag="ACHOTW_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication protein"
     gene            complement(4930..4992)
                     /locus_tag="ACHOTW_004545"
                     /pseudo
     CDS             complement(4930..4992)
                     /locus_tag="ACHOTW_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication protein"
     gene            complement(4989..>5369)
                     /locus_tag="ACHOTW_004546"
     CDS             complement(4989..>5369)
                     /locus_tag="ACHOTW_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFP9846510.1"
                     /translation="LSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNF
                     AGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQ
                     QAGVTASKPKLDLTNTDWIYGVDL"
BASE COUNT         1509 a         1304 c         1163 g         1393 t
ORIGIN      
        1 atatccctta agttattagc tttcaaatag cgactaataa actcagcgcc aaaatccgcc
       61 actatcactc tttcaggata tgtcaggaaa aaatctctag agctagagtc gacaagtaca
      121 gatgaacctc tctccagaaa cacactctct tttccgaaat aaacatcaaa ggactccaaa
      181 atcaatataa ctgaacatgt agttgccata tcatcccccc aatttaactg aaaccaggat
      241 gaagtatatg tatataaagt tcactttgcc aaacttcagt ataaaaaacc actaaaaata
      301 gggtgtgata aaaaataccg taacaataaa gcaaaggatt ataaattccg ttacagttac
      361 aaatgatact caagaaacaa ttccttagga aaaacttatt tacagccaat aagtaagaca
      421 cttatatgat atcaagtttt cataaaacat aactaggcta aatagctgcg cctaataccg
      481 ctacactttt gccagcccat gtttgcctcc gggtattgac cccttctcta cgcaacttca
      541 gttcccacca ccaactttgc ggcagctttg taggatcaat gtctaaaaga ataatggtga
      601 ccgataagaa aacgactgaa taactgcaga ttttcgctca aaaccttcct gtcagatcca
      661 tagcgaatca agtgctgaat gtcacagtat cgaacagaaa acagtgacga tctaaccctt
      721 caagaatatt ctacgattgt tctgtttagg aaaagcaaga cgggaagtcg ggagataagt
      781 cattgataaa gtggcggaga gagggggatt tgaacccccg gtagagttgc ccctactccg
      841 gttttcgaga ccgacccgat catcaaacga aacataaaat tagctcacat tatgaggaaa
      901 ggtatctttt tgcgctatgt aaattcaaag ggttagcctc attttcccga tggctttctc
      961 aacactacta gttgtgagcc cttgcaatgt tcattaatat acgtctcaca aataattcac
     1021 agatattgca aaatggatat tactgagttt ccttctggag taattgaaca ccttggctgg
     1081 tatgtatacc gattgattga tcctagggac ggaagcacct tctatgtagg gaaaggcaaa
     1141 ggtaaccgcg tatttgccca tatgcgcggt gaagtggcag cggctgatga tgacgactta
     1201 ctgagcaaca agctaaagca aatcagagaa ataagattag cgggacttga agttatccat
     1261 gtcatccatc gacatggaat gactgatgaa aagacggcgt acgaagttga agcagcactt
     1321 attgatgcct accctgggtt aacgaatatc atgaatggtg ctggcagcaa tgaattcggc
     1381 gccgcgcatg tcaaagagtt gatagcaaca tatcaacccg aaaccataac atttcatcat
     1441 aaagcattaa tgatttccgt taacagaagt gcaaaagatt cagagcttta tgatgcggtt
     1501 cgatttagct ggcgcattaa tgtctcgcgc gccagtaaag cagaagtcat tcttgctact
     1561 gtaaggggga tcgttcgagg agttttcatt gctgataaat ggctaaaatc aacgcgtgaa
     1621 cattttccta cgatgaaata ctgggacgaa gaccctgact ttgaggcaac acaaagctct
     1681 cgctatggtt ttgaaggtcg agaagcccca cctgaaatag caaaccttta tcttgggaaa
     1741 aaaataccag atgaattaag aaaaaaagga gctatgtctc cagttcgtta ctcacctaat
     1801 ttttgaatct tcaagtggta agcataaacc gcagcacgat cttgcatacg acgtgctacg
     1861 gtttcattta tctccggccg gaaacttctt atacagtgtc gatacaccaa catcatagat
     1921 gatcgctacc ttctggcgag gaattcctgc tgcaattaat cgtccggcct gcgcccattg
     1981 ttctggtgta agtttgggac gacgtccacc aattcgtccc tgtgcgcgag cagcttccag
     2041 tccagctttt gttcgttcaa caatcagttc tcgttccatt tcagccaggg cccccatcac
     2101 atgaaagaaa aagcgcccca ttggggtact ggtatcaata gcatccgtca ggctgcgaaa
     2161 attaacgcca cgttcgcgca actcctcaac cagaatgacc agatgccgca tactgcgccc
     2221 cagccgatcc agtttccaga caaccagagt gtcacctgcc gataatgtcc tgagcagttt
     2281 tttcagtccc ggcctttcgg actttgtacc gcttatcttg tcttcaaaaa tcagctcgca
     2341 tcctgcacag ttcagcgcat tacgttgtag atctgtgttc tggtcatttg ttgacacacg
     2401 tacatagcca ataagcatgg tagatctccc tgacaaaagc aggaatgatg ccatttgctc
     2461 gttatttctg cattttcata aacgttggtt tgggagaagc ggcaaaacgg gatgtgggga
     2521 caggggaaaa tcagataccg gacatggcct cttttgccag tggtaatgga tggatgaaat
     2581 tacccaacgg gaaaatcctg caatatggtc gtggtgcggt tacgccgaca ttatcgacgc
     2641 aaacaatgag aattacattc agcatccctt tccccaaaaa agcggactgc gccatgctta
     2701 ctcattctgg tgatggcggt gcgcctttag gcgctgggcg agggttcgtg atgactgcag
     2761 aaggcccaac gttaaccggc tttaattctg cttacagaac gtcatcaacc agcgacacgg
     2821 tatcgatgaa ttacagttgg tgggctgttg gtgagtaatt ttattcaggg tgatttatat
     2881 ggacgaatat gtttatagcg caaggcataa tgcttttttc cctgtggata tgattgataa
     2941 atataaatca gagggatggg atttatcaga cgctaaggaa gtaaatcaaa atattatcag
     3001 tgagtttatg gctgaaccgc cacaaggaaa aatccgtatt gccggagatg atgggctgcc
     3061 tgcgtgggca gatattcctc cacccacgca tgaagagctt attgaaatta ctgaatcaga
     3121 aagacagcta ctaattaacc aggccaacga atacatgaac agtaagcaat ggcccggtaa
     3181 agcggctatt ggtcgtctga aaggtgacga actgacgcaa tataatttgt ggctggatta
     3241 tctggacgca ctggagctgg tcgatacttc cggtgcgcca gatattgaat ggcctacgcc
     3301 tccggcagtt caggccagat gacatccggc gcggtgctgg tatctgttgc cgtcaccgcg
     3361 tcaatgtaat ccagcacggc gttaagtcgg gttgtttctg cctgagtcag tttccgtccg
     3421 gcctgtaact tcagctgaat cagactaatg gaagccattg ctgcatcaat cagtgattgg
     3481 cgctgtgctt ctgccgcgtc tactgcggca ctgtgttgtg cctcagtgtc tgtcacccat
     3541 ttctcaccat cccatttatc gtatggcgtt aacggtgaaa tcgtgacata accgtctttg
     3601 atggcaccga tataatccac tgtaacagct gcgccatttt cgattgagta aacagtctca
     3661 ttgcgatggt cttcttcatg gctccatccc ttacctgtaa atactgccac tcttcccgga
     3721 atgttttcgc cagggtcaat accagtggaa caggcgggca tacttacgcc agtattaata
     3781 tattcatcag accagcccgt atactcagat gtttcagcat cataataaaa acaacgcata
     3841 tcgcccggca ctgtagccag cccattttca tcaaaaacag gtttcattat ttagccctca
     3901 ccagaaagtt aaatgcaata tttcgcggtc tgactgcaac aaaattcaca ccatcaccca
     3961 cagagttact ggtgaaatta aatcgtgaaa atcctggctg atttccggcg atgccatcat
     4021 gaaagttaat tgcgtgtccc gcaccttcgc ctatattccc ggcaaactga gaaaagtttg
     4081 tagctgcctg ccagcttaat aattcgcgac caccgtctgc acctcgcccg tcatcccaga
     4141 tacgaatgaa atcaccgcgg gcttcaggta ataccagcga aggaaacact ttcgccagca
     4201 caggataatc agtggcagag aatttcgcgc cgttgaactt caaaaacacc atacaggacc
     4261 agctgtcgat tacagtattt ggcattgcag cggacggcca gaagaacgga acgccaatag
     4321 ctggagcacc ttctcccaaa ccaaggtttt gaagagccgt tttcaccgtg ccgtccgatt
     4381 tgatatcgcc aaacggattc ttgcggctca ggtattcaac agcaaacccc gatcccagca
     4441 attcaacaaa accgggcaga tcaccattat caagcacatc ccgttgcgtt ttatcactta
     4501 caaactgggc cagagctgca gcaataaagc tggcctgccg aataacctta ttgacttgcg
     4561 cactggatgc tttccctgct gtaaatccgg atataagcgc aggcaacgct tcccattcct
     4621 cctgcgacat aacattggca tttcgatcag ttgcaaacgc tttaaagtca tttttcgcca
     4681 tcagagtaat actccccatg cccctacatc aaaaccactg atgaattcgt tatccatatc
     4741 aaaaccaaaa aatttagagc cttccgatgg agtttccacc gaaggtgttt caatgccccc
     4801 cgcccatacc ccggcggctt ttactgttag atacccctgt ttaattgccg caattaactc
     4861 acgcgataca tctgaaatat cagtatcagg aaagacccag accgatatcg tcatgtcctg
     4921 gttatcgatg ttggcgatcc gacgcatctg ctcacggtca aagttaacca tctgtgcggc
     4981 gatgtttttc ataaatccac cccgtaaatc cagtcagtgt ttgtcaggtc gagttttggt
     5041 ttgctggctg tcacgcctgc ctgttgcttg ttacggttga tttcgagctg ggtccacttg
     5101 tcgcggagtt tggccggact cagcacgtta ccggaccaga agttgtcctg gcaggcccag
     5161 cggaaaagca cacacatatc gcggtggtta cgtccgtcac gttcacgcat caggcggata
     5221 tcgttagccc acccagcaaa attcggtttt ctggctgatg gcgcgatggt cttcaccatg
     5281 tcaaacatcc actctgcggc ggtcaggtct tctgctgttc cccacttgct gccgctctga
     5341 attgcagcat ccggtttaac cacagaaag
//
LOCUS       DAZKIF010000218         5323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZKIF010000218 DAZKIF010000000
VERSION     DAZKIF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.220"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            138..4040
                     /gene="espP"
                     /locus_tag="ACHOTW_004547"
     CDS             138..4040
                     /gene="espP"
                     /locus_tag="ACHOTW_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFP9846511.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4296..4777)
                     /locus_tag="ACHOTW_004548"
                     /pseudo
     CDS             complement(4296..4777)
                     /locus_tag="ACHOTW_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1609 a         1150 c         1192 g         1372 t
ORIGIN      
        1 tgtttgttat ccgtatgata acaaacacat aaaggagtaa acatttatct ggacgatgat
       61 tacaatgttc acgactgtca gagacacacc tctgatacat tagaaaaacc aatgtttccc
      121 ttaaaaatgg agcttatatg aataaaatat actctcttaa atacagccat attacaggag
      181 ggttaatcgc tgtttctgaa ttatccggca gagtatcatc aagagcaact ggtaagaaaa
      241 aacacaaacg catacttgca ttatgttttt taggcttatt acaatcctca tattcttttg
      301 cgtcacagat ggatatttca aatttctaca tccgtgacta tatggatttt gcacagaaca
      361 agggcatatt tcaggctggc gcaacaaata ttgaaatagt gaagaaagat ggctccaccc
      421 tgaaactacc ggaagtacca tttcctgact tctcaccggt tgcaaacaaa gggtcaacca
      481 catctattgg tggtgcatac agtatcacag ccacacacaa tacgaaaaac caccactcag
      541 ttgcgacgca aaactggggg aacagcacgt acaaacaaac tgactggaat acttcacatc
      601 ctgattttgc agtatcccga cttgacaagt ttgttgttga gacccgaggt gcgactgaag
      661 gcgcagatat ttcgttatca aaacagcagg cacttgaacg ttacggggtt aattataaag
      721 gagaaaagaa acttatcgca ttcagagccg gctctggtgt ggtatccgtt aaaaaaaatg
      781 gacgcataac tccatttaat gaggtttctt ataagccaga aatgttaaat ggctctttcg
      841 ttcacattga tgactggagt ggatggctga tattaaccaa caaccagttt gatgagttta
      901 ataacattgc ctctcagggt gacagcggtt cagcactgtt cgtctatgat aaccaaaaga
      961 aaaagtgggt tgtcgctgga actgtctggg ggatttataa ttacgccaat ggcaaaaacc
     1021 acgcagcata cagtaaatgg aaccagacaa ccattgacaa cctgaagaac aagtattctt
     1081 acaacgtgga tatgtcaggg gctcaggttg caaccattga aaatggaaaa ctgacaggca
     1141 ctggctcaga caccaccgat ataaaaaata aggacttaat atttactggc ggtggagata
     1201 tcctcctgaa atcctctttt gataatggtg ctggcggtct tgtctttaat gataaaaaga
     1261 cctatcgagt aaacggggat gatttcacct ttaaaggtgc cggtgttgat acaagaaacg
     1321 gcagcaccgt tgagtggaat atccggtatg ataataaaga caaccttcac aaaattggtg
     1381 atggcacatt agatgtccga aaaacccaga acaccaacct gaaaacaggt gagggtcttg
     1441 tcattcttgg agctgaaaaa acattcaata atatctacat aaccagtggt gatggaactg
     1501 tccgactgaa tgcagaaaat gcactgtctg gcggtgaata caacggtatt ttctttgcga
     1561 aaaatggcgg aactcttgac ctgaacggat ataatcagtc tttcaataaa attgctgcaa
     1621 ctgattcagg tgctgtaata accaatacgt caaccaaaaa atccatttta tccctgaata
     1681 atactgctga ctatatctat cacggtaaca taaacgggaa tctggacgta cttcagcatc
     1741 atgagacgaa aaaagagaac cgtcgtctta ttcttgatgg gggcgtggac acaacaaatg
     1801 atataagcct gcgtaataca caactgtcca tgcagggaca tgccactgaa catgccattt
     1861 atcgggatgg agctttctct tgttcactac cagctcctat gcgctttttg tgtggcagtg
     1921 attatgttgc aggaatgcaa aatacagaag ctgatgctgt aaaacaaaac ggaaatgcct
     1981 ataaaaccaa caatgctgtc tctgatttat cgcagccaga ctgggaaacc ggaacattca
     2041 gatttggaac gctacatctt gaaaattccg atttttctgt tggtcgtaat gcaaatgtaa
     2101 tcggggacat tcaggccagt aaatcaaaca ttactattgg tgacactaca gcatatattg
     2161 atttgcatgc tggtaaaaat attaccggtg atggttttgg cttccgccag aatattgtgc
     2221 gtggaaactc acaaggagaa acgctgttta caggagggat cacagcagaa gacagcacta
     2281 tcgttattaa agataaagca aaagcattat tttcaaatta tgtatacctg ctgaacacaa
     2341 aagcaaccat agagaacggt gctgatgtga caactcaaag tggtatgttc tccacgagcg
     2401 atatcagcat ctctggtaat ctgtccatga caggcaatcc cgacaaagac aataaattcg
     2461 agccctcaat atatctgaat gatgcttctt atctactgac tgacgactcc gccagactcg
     2521 ttgccaaaaa taaagcatct gtggtgggag atatacactc cactaaaagt gcatccatca
     2581 tgtttggtca tgatgaaagc gacctctcgc agttgtctga cagaacctca aaagggcttg
     2641 cacttggtct tttaggtggc tttgatgtct catatcgcgg ttcagtcaat gccccgtcag
     2701 catctgccac tatgaacaac acctggtggc aactaaccgg agattctgcg ctgaaaacac
     2761 tgaaaagtac aaacagcatg gtctatttca ctgacagcgc aaacaataag aaattccata
     2821 cgctgacggt cgatgagctg gcaaccagca acagcgccta tgcgatgcgt acaaaccttt
     2881 ctgaatcaga caaactggag gtcaaaaaac acttgtctgg tgagaacaat attttactcg
     2941 ttgatttcct tcagaaacca acgcctgaaa aacaactgaa tattgaactg gtaagcgcgc
     3001 caaaagacac caatgaaaat gtctttaaag ccagtaaaca aaccattggt ttcagtgatg
     3061 taacgccggt cattacaacc agggaaaccg atgacaaaat aacatggtca ctgacaggct
     3121 ataacacggt agcaaacaag gaagcaaccc ggaatgccgc cgccctgttc tctgttgact
     3181 ataaagcgtt tctgaacgag gtcaacaacc tgaacaaacg tatgggtgac ctgcgtgata
     3241 tcaacggcga agccggtgca tgggcacgca tcatgagcgg taccggctct gccagtggtg
     3301 gtttcagtga caactacacg cacgttcagg tcggggtcga caaaaaacac gagctggacg
     3361 gactggattt gtttaccggt ttcactgtca cacacactga cagcagtgcc tccgccgatg
     3421 ttttcagtgg taaaacgaag tctgtggggg ctggcctgta tgcttccgcc atgtttgatt
     3481 ccggtgccta tatcgacctg attggcaagt atgttcacca tgataatgag tacactgcaa
     3541 cctttgccgg actcggaacc cgtgattaca gcacgcattc atggtatgcc ggtgcagaag
     3601 cgggctaccg ctatcatgtc actgaggatg cctggattga gccacaggct gagctggttt
     3661 acggttctgt atccggtaaa cagtttgcat ggaaggacca gggaatgcat ctgtccatga
     3721 aggacaagga ctacaatccg ctgattggcc gaacgggtgt ggatgtgggt aaatccttct
     3781 ctggtaagga ctggaaagtg acagcccgtg ccggtctggg ctaccagttc gacctgctgg
     3841 ctaacggcga aaccgtattg cgggatgcat ctggtgaaaa acgcatcaaa ggtgaaaagg
     3901 acagccgtat gctgatgtcc gttggcctga atgcagaaat cagggataac gtccgctttg
     3961 gactggagtt tgagaaatcc gcctttggta agtacaacgt tgataatgct gtcaacgcta
     4021 atttccgtta ctcgttctga tactccgtat tcaaaagggc ctgcgggccc tttacgctca
     4081 gtgcctgccg ccctgatatg tctgagagac atttccggga tgtcagtcga ctggtttact
     4141 taactgcgat gactaaagct ggcagtaatg ccggcttttt tacggccaca tacgtcttcc
     4201 tctcatccgg aactgtgata caaatcctgc atacaggatt gaccgccggt tcctgcaaat
     4261 ggcacatttt cagtaagata gcgaaggtta catttgtcac tggttaacat accgatggta
     4321 aaaggggcaa gcagcgccag caattcatca gcccgggggc caaaagtgta ggccagaacg
     4381 ccaccagttt tcgtgttgta cgcgtaccag agcctgtgct gtcacgcttt actgccgata
     4441 aaactccatt gctcatcaag ttcgcagatg agtgccatat cagcatgagc gaccggggat
     4501 gacgttattc ttcgtggcga gcgtttttta aagtccgaat gacagtatta atgccgattt
     4561 tcagcgtacg ggcagtatcg cgaactccgg caccgttgaa aggcatttca gtgatttggt
     4621 cttgaactcc cggtttacgg gcctcgtaac ggtaagtgag ctgaaacact cgatgacagt
     4681 cacggcagcg aaatcgatca tgtccttttg ggttcttccc gtgacggtaa acctgaacgg
     4741 actggcaacg ggggcaatga aggttaactg tgaccattaa agaaccttaa aagtgactat
     4801 tataccccta attcaactaa ttggaggcat taccatgcat tagtgcaagc gccttgtgga
     4861 ttcttttgct aatggcggaa acatattgta caatattgca tttatccctc aataaataaa
     4921 tgagttatca gattgcaaaa atctgttatc aaactaattc aaataaaaag agattgatac
     4981 gaccaacgta cacaaaaatt ttcgatattc gaactaaata acagctccta ataccgctac
     5041 actttagcta tccagtctgc gcctcagaaa tggacgatgc cgttgaagga ctggtgtatg
     5101 gcaatgagcc gctttattat cgaattcggt gaccgcttgg acggtcactt cggagaaaag
     5161 gcatttacac agcatcctaa acaggttcag cataggtgat ataccgcctt ttcattttct
     5221 tctccattat ttataaggga ggtgtattat tatgcaatat tattggtttt atccattgtt
     5281 tttagttcat gaatgtttgc aaaaaaaata aatgaacaat aat
//
LOCUS       DAZKIF010000219         5308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZKIF010000219 DAZKIF010000000
VERSION     DAZKIF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.221"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..67
                     /locus_tag="ACHOTW_004549"
     CDS             <1..67
                     /locus_tag="ACHOTW_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846512.1"
                     /translation="ETLLQQPEERFAWLRSKKDRF"
     gene            139..1497
                     /gene="mltD"
                     /locus_tag="ACHOTW_004550"
     CDS             139..1497
                     /gene="mltD"
                     /locus_tag="ACHOTW_004550"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP9846513.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(1545..2315)
                     /locus_tag="ACHOTW_004551"
     CDS             complement(1545..2315)
                     /locus_tag="ACHOTW_004551"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9846514.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(2393..3193)
                     /gene="yafD"
                     /locus_tag="ACHOTW_004552"
     CDS             complement(2393..3193)
                     /gene="yafD"
                     /locus_tag="ACHOTW_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9846515.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3434..4348
                     /gene="yafC"
                     /locus_tag="ACHOTW_004553"
     CDS             3434..4348
                     /gene="yafC"
                     /locus_tag="ACHOTW_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP9846516.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4345..5148)
                     /gene="dkgB"
                     /locus_tag="ACHOTW_004554"
     CDS             complement(4345..5148)
                     /gene="dkgB"
                     /locus_tag="ACHOTW_004554"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP9846517.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1394 a         1274 c         1321 g         1319 t
ORIGIN      
        1 agaaacatta ttgcaacaac ctgaagagcg ttttgcatgg ttaaggtcaa agaaagatag
       61 gttctgataa aactttcttg tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa
      121 gcaactattg acgcacacat gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg
      181 ggttgccaga gtaccggcaa cgttcaacag cacgcacaga gcctttctgc agctggtcaa
      241 ggggaagcag caaagtttac aagtcaggca cgatggatgg acgatgggac gtctatcgcg
      301 ccagatggtg acttgtgggc tttcattggc gacgagctaa agatgggaat tccggaaaat
      361 gaccggattc gcgaacagaa acagaaatat ttacgcaata agagctatct ccacgatgta
      421 actttacggg cagagccgta tatgtactgg atagccgggc aagttaaaaa acgtaacatg
      481 cctatggaac tggtactact acccatagtg gagagcgctt ttgatcctca cgcaacgtct
      541 ggcgccaatg ccgcaggcat ctggcagatc attccgagca cggggcgcaa ttatggtttg
      601 aaacagaccc gcaattatga cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg
      661 aatatgatgc agcgtctgaa caaaatgttt gatggcgact ggcttctgac cgtagcggct
      721 tataacagcg gcgaaggtcg ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa
      781 tccacggact tctggtcgtt accgttgccg caggaaacga agcagtacgt gcctaaaatg
      841 ctggcattga gtgatattct caaaaacagc aagcgttatg gcgtacgtct gccaacgacc
      901 gatgaaagcc gtgctctggc gcgtgtgcac ctgagtagcc cggttgaaat ggcgaaggtt
      961 gcagatatgg cggggatttc cgtcagcaag ctgaagacat tcaacgctgg cgtgaaaggc
     1021 tccacgctgg gcgcaagtgg tccgcagtac gtgatggtgc caaagaagca tgcagatcaa
     1081 ctgcgtgaat ctctggcttc aggcgaaatt gctgctgtac agtcgacgct ggttgccgac
     1141 aatacgccgc ttaacagccg tgtttacacc gtacgctctg gcgacacgct ttcaagtatc
     1201 gcttcacgtc tcggcgtaag caccaaagat ttgcagcagt ggaacaaact gcgcggctct
     1261 aagctgaaac caggccaaag tctgacgatt ggcgcaggta gtagcgcaca gcggttggca
     1321 aacaacagcg atagcattac gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct
     1381 aaacgccacg gcgtgaacat caaagatgtg atgcgctgga acagcgatac tgcgaatctg
     1441 caaccaggcg ataagctgac gttgtttgtg aaaaacaaca gcatgccaga ctcctgacaa
     1501 accagataat agaaaggcac cgattccccc ggtgcctttt ttatttatgc cgctttatgt
     1561 gcatctacca tgatgatatc actggtgaaa gagccatcat tctgcaaggc aaaatacgtt
     1621 ctcacctctg tcgatgcgct ctgttggtaa atgcgaatag cgtctactaa cgcttctggc
     1681 gtacgcattc tcgcgaccca tgaagaaaat tccagcggta acttatctgt aattaaatta
     1741 tcaactatca gattggcttc attgattaac ctcaaccact caccgctggc gtagtttcgt
     1801 acgtgagagg tatcgcataa tgcttctacc gtctgtaacc agatgtcgcg cactgggtgg
     1861 cccggagaca ttacgtccat cacaatcagc ctaccgccag gtttcaatat ccggttcact
     1921 tcacgcagtg ctgcaccaac atcatgccag tgatgggcag aataacggct gataacaata
     1981 tcaaatgcgt tatcggcaaa tggcagactt tcggcatacc cctggcgggt ggtgatattt
     2041 ttcagttgcc ggacttcggc agcttgtgcc acgacatcca gcatttgggc agataagtca
     2101 tacgccacca ccgtgctcac attttgcgca gcgacaaagc tggcatgccc tgctccgcag
     2161 cccatatcaa gtacacttgc atcaggataa tcagccagac acaccgccag gagctgtaaa
     2221 tctcgtccgg atgcatgtac ggtactggtc agatattcgc tggcttgaga gctaaactgc
     2281 ttttctacgt ggtcatggtg ggattgtgtt gtcattgcac tgtccttttg ttgtttgagt
     2341 ataaaagggc agctccaaac gttggtctgc cctgtggcag acctgacata ccttatttat
     2401 caggcttgcc gggactgaat tcaacgagta gcggattgtg atcggacgcg cgcgtaacca
     2461 gtacagaagc ttcgctgacg ttcagaccac ggtagaaaac aaaatcgagc gggcgaccaa
     2521 acgcccgacg gcgctgatca tcggtaaaac gcacctgacg cagcgacatt tcccgcgcaa
     2581 aacgatataa cgcgttcatc cttctacggc tccaagcatt gaaatctccc gccataatga
     2641 ccgggccgct gtggtgagct atctgatcac caataggaag taactgctta ctataaacat
     2701 ccacgcccag actgaagttg acggcgtgta tattaaccac cattaacagg cgggtgtcag
     2761 gtaatggata gaccgtcacc agtgccgact tcgccagacg caaaatgggt tctcgttcac
     2821 gtaacgggca gcaatacact ggatgtgccg ccgaaagggt cattacgcca gaaggatgtt
     2881 gtggcagcac gaaagcgggt acctgatcgg cggcaagata gttagcagtc gcaaactgta
     2941 ccaactctgg cgttgtttgc gcttcctgca ataacaccag atgtgcgtct ttgccgtaat
     3001 tctttaatac cgacaaccat tcagcgcgtt gctgtttgta tatgttccac accaggatcc
     3061 gaatacgctc ttcggtactt aacggttctc cgggtggtaa tgcctggccg atgctcgcaa
     3121 aagaccccgg cggtaagatc ctctccgcag gttgtccggc aacatagcgc atggcatagg
     3181 tgttttttcg cacttttgac tttgaaacct ctattacgtc aacccttccg cactcgggag
     3241 ggttctactg ttatagggat tttagctcac tctttcaacg agcaattgta tattgttatg
     3301 taagcaagtg cttacaccgg tcagaatttt gctcattatt gagaacatta aatcggatga
     3361 catatcacgc atcctgatgg tttaatgaca aataaattca aaatatctca tctattttgc
     3421 aacaggagca aaaatgaaag ccacgtcgga agaactcgcc atttttgttt cggtcgtcga
     3481 aagcggcagc tttagccggg cagcggaaca attagggcaa gcaaactcag cggtaagccg
     3541 ggcggtgaaa aagctggaga tgaaacttgg cgttagcctg cttaatcgga ccacgcggca
     3601 acttagcctg acggaagaag gcgagcgtta ttttcgtcgc gtacagtcaa ttttgcagga
     3661 gatggcagcg gcagaatcag aaattatgga gacgcgtaat acaccgcgtg gactgttgcg
     3721 catcgatgcc gcaactccag tggtgctgca ctttctgatg ccgttgatta agccttttcg
     3781 tgaacgctat ccggaagtca ctttatcgct agtctcctcc gaaacgatta ttaatttgat
     3841 cgaaagaaaa gtggatgtcg cgatacgcgc cggtacgtta acggattcca gcttacgtgc
     3901 caggccgtta tttaacagtt atcgaaaaat tatcgcctcc cccgattata tttcccgcta
     3961 cgggaagcca gaaacgatcg acgatttaaa gcaacatgtt tgcctgggat tcactgaacc
     4021 cgcttccctc aatacctggc cggtagcctg tagcgatgga caattacatg aggtgaagta
     4081 cggtttgtca tccaatagtg gggaaacact gaaacagctt tgcctgagtg gcaacgggat
     4141 tgcgtgtttg tccgactata tgatcgacag agaaatcgct cgcggagaat tggtggagtt
     4201 aatggcagat aaagtgttgc cagtggaaat gccattcagt gccgtctatt acagcgaccg
     4261 tgcggtaagt acgcgcatcc gggcttttat cgatttcctt agcgagcatg taaaaacagc
     4321 tcccggagga gctgtcagag aggcttaatc ccattcagga gccagacctt ccgggctaac
     4381 caggcggtcg ttgcaatcca gtgcggcgat cgctttttta tcttcggcat caagctgtaa
     4441 attttgtgcc ttaagattac tctccaggtt ttcacgttta gtagaagaag gaattactga
     4501 gtaaccttcc cccatagccc acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc
     4561 tgcgatacga gcaataacct catctttcag ggccttacca tacgccagcg tcatatagga
     4621 agtaatatgg atgccgtgct gtttagccca ggcaaccact ttacggtttt gcagataagg
     4681 agagagttca atctggttag tagcgatgtt ttcagcgcca acagcagcaa tcgccttttc
     4741 catcaatggg atcgtgaagt tggaaatacc gatctcacgc gtcaaccctt cttttttggc
     4801 ttccagcagc gcctgcataa actcttcaac agagacttca tcgtttggtg acggccagtg
     4861 gattagcgtc agatcaacat aatcggtacg caatttttgc aggctctctt ttagactcgg
     4921 gatcaatttg tctttgctga gattttcaat ccagatttta gtggtgatgt agagttcatg
     4981 acgtggcacg ccactttctg caatcgccag acctactgcg gcttcgttat catagatttg
     5041 tgcagtatca attgcgcgat aaccaagttc aagcgccgtt ttcacagatg aaataacaac
     5101 gtcgtctttc agacggaaag tacctaaacc aaatgcaggg atagccataa tatgcctctt
     5161 ttaatgctat tttcgttaac tgaggtgatt atgggcttta gaatgttgcg gaaaaagggc
     5221 gtaaaacgca gaggattttt gcgattttag caataataga aatgtagacg aaaaaaaacc
     5281 tcgagcgata actcgaggct tcttaata
//
LOCUS       DAZKIF010000220         5257 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZKIF010000220 DAZKIF010000000
VERSION     DAZKIF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.222"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            89..565
                     /locus_tag="ACHOTW_004555"
     CDS             89..565
                     /locus_tag="ACHOTW_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP9846518.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            562..2685
                     /locus_tag="ACHOTW_004556"
     CDS             562..2685
                     /locus_tag="ACHOTW_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9846519.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            2682..2894
                     /locus_tag="ACHOTW_004557"
     CDS             2682..2894
                     /locus_tag="ACHOTW_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846520.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVIFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            2894..4396
                     /locus_tag="ACHOTW_004558"
     CDS             2894..4396
                     /locus_tag="ACHOTW_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9846521.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            4341..>5257
                     /locus_tag="ACHOTW_004559"
     CDS             4341..>5257
                     /locus_tag="ACHOTW_004559"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9846522.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNE"
BASE COUNT         1224 a         1243 c         1635 g         1155 t
ORIGIN      
        1 gttttttgtt cgacatcttt atatggcggc atttctttac aaaaaaacag agtcacttct
       61 gttctgcggt ttatgtggag ggatgtaaat ggacggcgag ctgaaaaata tgaagttaaa
      121 tattaatcaa ctggcagccc tttcaggtct gcaccggcag actgttgccg ccagaatggc
      181 ggatgttcct cttgcaccag gcagtaatga aaagaaaaaa ctgtatctcc tgacggattt
      241 gataacttcg ttgctggaaa aaccaccatc ttccgaagat gaggatatgg atcctcacgc
      301 tcgtaaggca tggtatcagt ccgagcgcga gcgtcttaaa tttcagcatg aaactgttca
      361 gcttgtgcca gtcagtgatg tcaggcggtc cttttctgtc gtggtgaaag cgatagttca
      421 ggtactggaa acctggcctg accggctgga gagggacagg gggtggactg catcacaact
      481 gaatgaagta cagattgtgg ttgatgagat ccgcgacaca ctggaaaagg cagtcattga
      541 ctgttgtgat gaggccgata tgtgaatcag gtgaacgaga gccatagccg cgcatccgat
      601 atctggcgcg aagtggcctc gctgtttcgc ccacccagcc ggttaccagt agcggaagcc
      661 atcaggcgtt atatgcgggt tccacgggga gccaatactt ccggtccgtg ggagtcatcg
      721 ctgacgccct atatgattga ccccattaat acattatcag cccgtgaata tgacgcggtg
      781 gtgtttgtgg gacctgcgcg aaccgggaaa accgaagggc tgattgatgg ctggattgtg
      841 tacggcatca tctgtgatcc ggcggatatg ctggtggtgc agatgactga gacgaaggcg
      901 cgtgagcatt ccagaacgcg tctttccagg acgtttcgcc acagtccgga ggtcagcaag
      961 cgcctcagtc cttcccgtaa tgacaacaac gtccacgata aaatgtttct tgacggctcc
     1021 ttcctgaaga ttggctggcc gtcgatcacc gtcttttcct cttcggatta ccgtcgtgtg
     1081 gcgctgacgg attatgaccg tttccctgaa aatgtggacg gggaagggga tgccttcacg
     1141 ctggcctcaa agcgtaccac cacctttatg tcctcgggga tgaccctggt cgagagttca
     1201 ccggggcggg atatcaccga taccaaatgg cgttgtggtg gcgcacatga ggcaccgcca
     1261 acaacgggga tcctgtcact gtataaccgg ggagaccgcc gccggtggta ctggccgtgt
     1321 ccgcactgcg gggaatattt tcagccggtg atggataaca tgaccggata ccggaataac
     1381 ccggattttg tggctgccgg tcaggctgcc cgtctgatgt gtccgcattg tcgcgggctg
     1441 attgcccctg agcagaaacg cgaactgaat aaccagggga tctggcttcg tgaaggtgaa
     1501 cgggcggcgg cggacggcag tatcaccgga acgccacgaa actcccggat tgcgtcattt
     1561 tggatggagg ggccagctgc ggcgtttcag acctgggaac aactgatttt taaactgctg
     1621 gcggcagaag aagagtatga gcgaaccggc agtgaagaga ccctgaaagc ggtggtgaac
     1681 accgatatcg gacgacccta tctgccccgt tcagccacgg aacagcgtaa aagtgaactg
     1741 cttgaacagc gtgccgagcc gtttccccgg cgatctgtgc cggatggtgt gcgttttatt
     1801 gaggcaacgg ttgacgtaca gggcggtaaa aatcgccgtt ttgttgtgca gatcaccgga
     1861 tacggagagc agggggaacg ctggattgtt gatcgctaca acatccggca ttcactgcgc
     1921 tgcagtccca acggtgaaag tctgccggtt gatccggcgg catatccgga ggactgggat
     1981 ttgttgctga cggatgtgtt ccataaaaca tggccgctgg cttctgatcc ggatgtgcgc
     2041 atgcgtctga tggccatggc ggtggatacg ggaggggaag ccggggtgac agataacgcc
     2101 tatcgtttct ggcgtcgttg ccggagtgac ggactgggca acagggtgtt tctgttcaag
     2161 ggggatggac ttcgccgtga caggctgatt aaccgaacct tcccggataa taccggcaga
     2221 agtgcccgcc gtgccagagc cagtggcgat gtcgcgctgt ggctggttca gacggatgcg
     2281 tttaaggatc gtgtaaataa tgccctgtgg cgtgacacac cagggccgaa ctatatccac
     2341 tttcccgact ggctggggcg gtggttttac gatgagctga cctatgaaga gcgcggcagt
     2401 gacggaaaat ggcgaaaacc gggcaggggc gctaacgaag cgtttgacct gctggtttat
     2461 gcggatgcgc ttgccgttct gcatggttac gaaaagatcc gctggccctc cgcaccggac
     2521 tgggcacagc gggaaacgtg gctcgtcttc ccgcaggagc gttctggtga aacggtatcc
     2581 ccggaactga cggccggggc agaaaaacgc cgtcgccgga agaaaaaact gcggacggag
     2641 cgtgcggaag ataatccatg gataacatca ggaggctggt tgtgagcaca gaagaagcca
     2701 gagaaatgat acagcggtac cgtgaagcgg aaatggccgt actggaggga aagtctgtca
     2761 tcttcaacgg gcagcaactg acgctggaaa gcctttctca gatccgcgcc ggacgtcagg
     2821 agtgggaacg caggcttgcc gcgatggtga gccgcaggcg gggaaaaccg ggatttaaac
     2881 tggcgaggtt ttaatggcaa ttattgatga tgtgatcggc gtgttttccc ccgggtggaa
     2941 agcagccaga ctgcgttcaa gggcgttaat catggcctat gaggcggtga aaccgacccg
     3001 gacacataaa gcccggcggg aaaatcgctc tgctgatcag ctcagtaaat acggtgcggt
     3061 ttccctgcgg gagcaggccc gttttctgga tatcaatcat gacctggtga ttggtgtgtt
     3121 tgacaagctg gaagagcggg tgattggtgc caggggaatt attgtggagc ctcagccatt
     3181 acgaaaaaac ggggaaatgg cggctgagct ggctgcggat atccgccgtt tgtgggctga
     3241 atggtccgtg agtccggatg tgacagggca gtatacccgt cctgtgcttg aacgtttact
     3301 gctgcggacc tggctgcggg atggtgaagt gtttgcgcag atggtcagtg gtgcgggaaa
     3361 cggtctggaa cggacggcgg gagtgccatt ctggcttgag gcgatggagc cggattttgt
     3421 tcccatgcgc actgatgaat ccgccggact gaatcagggg gtttttcttg atgagtgggg
     3481 aagaccgaaa aaatatctgg tttataaaaa ttatccggtc agcggccggc agagtgatac
     3541 gaaagaaatc gctgccggaa aaatgatcca cctgaagttc actcgtcgtc tgcatcagac
     3601 gcgaggctca tccatgttat cgggggtgct gatgcggatc agtgccctta aggagtatga
     3661 ggatgcggaa ctgacagcgg cgcgtattgc tgcggcgctg ggactgtata tccgtaaagg
     3721 tgacggacag gactatgaag atccggggat caaagagacc gagcgggaag tccatatcac
     3781 cccgggtatt atttatgacg atttgcgcaa gggcgaggat atcggcatgg tcaaatctga
     3841 ccgtcccaat cccaaccttg aaactttccg caacggccag ttgcgtgcag tggcagcagg
     3901 cagtcgtctg agtttttcca gtgcggcgcg taactataac ggcacctaca gcgcccagcg
     3961 gcaggagctg gtcgagtcca cggatggtta cctgatcctg caggactgtt ttattggcgc
     4021 ggtaacccgc ccggtgtacc ggacatggct gaatatggtg gttgcggcag gtctgctgaa
     4081 aattccggcg gatgtggaga tgaaaacgct atataacgcg acgtattccg gtccggtgat
     4141 gccgtggatc gacccggtta aggaagctga agcctggaga attcagatcc ggggtggtgc
     4201 agcgacagaa tctgactggg tgcgtgccgg tgggcgcaat ccggatgagg tcaaacgtcg
     4261 ccgcaaggct gaaattgatg aaaacagcag actggggctg gtctttgata ctgaccccgt
     4321 caacgacaaa ggaggcaaca gtgccggaac tgaacgacag tatcagcgcg acaccgaaag
     4381 ccagcatgaa gaataaatcc tggttcagga tgcaagctgg ggggccgggt gacgcggata
     4441 tttatattta tgacgagatt ggtttctggg gagttaccgc gaagcagttt gtcagcgaac
     4501 tgaatgcact gggtgatatc acccacatta atctccatat caattcaccg ggtggcgatg
     4561 tctttgaagg catcgccatt tttaatgccc tgaaaaatca gggggcgacc attaccgtgt
     4621 atgtggatgg cgttgccgcc tcgatggcat ctgtgattgc gatggccggt gatacggtca
     4681 ttatgccgga aaatgccttc atgatgatcc ataagccatg gggattcagt ggcggggatg
     4741 ctgaggatat gcgcagttat gccgatttgc tggataaagt cgaatcggta ctgttgccag
     4801 cctatgcgca gaaaaccgga aaaaccaccg atgaaattgc cgccatgctg gcggatgaaa
     4861 cctggatgtc cggtgccgaa tgtctggcac acggatttgc tgaccaggtg acacccgctg
     4921 ttgaggcaat ggcatgtatt cagtcaaaac gtacagagga atttaaaaag atgccggaat
     4981 ccatccgaaa catgattact ccgccacgca acagtgcccc gcgtgatacc acagtgacaa
     5041 tccctgcacc ggcggtaaca gaaccatcac cggtaccggc agtgtctgat gaggcgacca
     5101 ttcgcgcccg cgttatggct gagcagaaag cccgcatgtc aggcattaac gatctgtttg
     5161 ccatgttcgg cggtcgctat cagacgcttc aggcacagtg cgtggctgat cctgactgtt
     5221 cgctggaaat ggcccgtgaa cgtctgctga atgaaat
//
LOCUS       DAZKIF010000221         5146 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZKIF010000221 DAZKIF010000000
VERSION     DAZKIF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.223"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(33..1535)
                     /locus_tag="ACHOTW_004560"
     CDS             complement(33..1535)
                     /locus_tag="ACHOTW_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP9846523.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(1665..2684)
                     /gene="ahr"
                     /locus_tag="ACHOTW_004561"
     CDS             complement(1665..2684)
                     /gene="ahr"
                     /locus_tag="ACHOTW_004561"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP9846524.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            2880..2964
                     /locus_tag="ACHOTW_004562"
     tRNA            2880..2964
                     /locus_tag="ACHOTW_004562"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2914..2916,aa:Leu,seq:caa)
     gene            3055..5004
                     /locus_tag="ACHOTW_004563"
     CDS             3055..5004
                     /locus_tag="ACHOTW_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HFP9846525.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECGDKLMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNF
                     FPPDDKGEREQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLG
                     LMTGARVNEIAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELG
                     FIEYVDALKAAGYDRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERDRSKSFHSF
                     RHSVSTLLKEKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEI
                     ARFNIPDGLDAVRDALRRKRGKQTG"
BASE COUNT         1268 a         1338 c         1256 g         1284 t
ORIGIN      
        1 gtcgcatcag gcaaagacaa atctcgcctg acttaccttc ttctcccccc cagcaaactc
       61 cccaacatcc cgcgtacaat ctgattcgtc acttggcgag cggcgctttt ggccattgtt
      121 tgcaccacac catctttctt cccgccgcgt ggtccggtag tgccgaacaa aatatccttc
      181 aatccaccaa gaatgccgtc atccaccgct acctctttcc ctttcgcggg gggattattt
      241 tgctgctcgg tactggcctg aaagcctttt tgcaacatct cataggcgga ttcccggtcc
      301 acctcatcct catatttgcc atacaccgga gagtgattaa tcaagccatt acgctcatct
      361 tccgtcaccg gccccatccg cgaacaaggc gcgatcacca tcgcacgctc caccacagaa
      421 gggcttcctt tcgcatccag aaaagagatc aacgcctcgc cggtgcccag ttcctgaatc
      481 gccttttcgg tatcaaacgc cggattggcc cgcatggttt gcgccgcagc ttttactgct
      541 ttctgatctt tgggcgtaaa agcccgcaaa gcgtgctgaa cgcgattacc gagctggccg
      601 agcacattat ccggaatatc agacgggttt tgcgaaacga accagacgcc cacgcctttt
      661 gagcgaataa gccttatcac ctgctcaatc ttatccagca gtacctgcgg tgcatcgtta
      721 aacagcagat gtgcttcgtc gaagaaaaac accagttttg gtttctccag atcgcccgct
      781 tccggcaatt gttcatacaa ctctgagagc atccacagca ggctggcggc gtacagtttc
      841 ggcatctgat aaagtttctc ggcgctgagg atattgataa cgcctttacc gttggcatcg
      901 gtgcgcatcc agtctttgat atccagcatc ggctcaccaa agaagtgtgc tgcgccttgc
      961 tgttccagcg acaacagccc gcgctggatg gcaccaaccg atgcgctact gatatttccg
     1021 tactgattct ggaaagattt ggcgttatcg ccgatgtact gggtaatcgc ccgcagatct
     1081 ttaaagtcga gcagtaacag cccctgatcg tcagcaatgc ggaagatgat attcagcacg
     1141 ccagattgca catcgttgag attcaacagc cgcgccagca acagtggccc caggtctgaa
     1201 accgtcgccc gtaccgaatg gcctttctcg ccaaagatat cccacaccac caccggatta
     1261 gcatgcggtt gccagtcatt gacgtcgata tttttaagcc ttgcgagcag tttttccgac
     1321 gccgttcctg cctgcgcgat accggtcaga tcgcctttca catcagccat aaacaccggc
     1381 acgccgattt ccgacaatga ctccgccagt ttttgcagcg tgacagtttt acccgtcccc
     1441 gttgcgccag taatcagccc gtgacggtta gccattcccg gcagtaaaaa tagttctgtg
     1501 tccggcgtgc gggcaattaa caggggttcg ctcatgagat atcctccagt ttcctgcctg
     1561 gagtataggc aacggctgcg cactcttcac cgctaaattc agcagattgc gagacgcctt
     1621 ccaaagagat ttaagtgccg ggcgacaggc acctggcaaa gagatcagaa gtcagctttc
     1681 agaaccacgc ggtaacgcgc cttaccgtcg cgcacatgct tgatggcatc gttaattttc
     1741 gacatcggga acaattcggt agtcggcgca accttgctgc gggcggcaaa gcgcatcagc
     1801 ttacgcaact cataaggtgt accggtagca gagccagaga cactgcgatc gcccgcaatc
     1861 agcgtaaagg ccggaaccgg caacggcgtg agaaccgcac cgaccgtatg gaaattaccg
     1921 ccataggtca gcgcctcaaa ataaggctgc cagtcgaggc tgacgttcac agtattgata
     1981 atgagatcaa actgccccga cagggctttc agtgcctgcg gatcgcggct attcaccact
     2041 ttatcggcac ccatcgccag tacttcctgc tctttcgccg gattagaact aaaggccgtc
     2101 acctcgcatc ccattgcgtg cagaagtttt atagcgatat gccccagccc gccaatacca
     2161 attaccccaa cgcggctggt agcagtgata tggtgcatca acagtggttt aaagaccgtg
     2221 ataccgccgc acaacagcgg cccggcggac tcgatatcaa tattttctgg cagtggaatc
     2281 acccattgcc agtccgcacg caacttctcg gcaaagccac cgcgattcat aatcgtcggc
     2341 acagcacctt gctcgcagtt gatctgatta ccgctaatac aggcgtcgca gtgaccacag
     2401 ctacgcgccg tccagccaat cccgacacgc tggccgacct gcaaaccttt atcctgcgcg
     2461 gcgctcccga gcgccaccac gcgcccaatc acctcatgcc cggcaaccag cggatattgt
     2521 gaaaatcccc attcattgtc gatcatcgat agatcggaat ggcagatccc gcaataatcc
     2581 acctgcactt caacatcttg tggcctcagc tcaccgggat cgtactcata aacttccagt
     2641 tcgccgcccg cttcttttgc ggcatagctt tttatcatcg acattttttg gtccttctct
     2701 ggtgttgttt gggaagtgta gagcatggca gggcggggtg cctggagtgt gacaaaggtt
     2761 acacatcgct gtatgcaatg ctgaaaattt cagcacttag cgaggtgcga gcaagctggc
     2821 gcttgcatgg tggcgtgcga caggtataat ccacaacgtt ttccgcatac ctcttcagtg
     2881 ccgaagtggc gaaatcggta gacgcagttg attcaaaatc aaccgtagaa atacgtgccg
     2941 gttcgagtcc ggccttcggc accaagactc atctaaacta ttgttttaaa acaatttaat
     3001 tcataatctt gcttgaaatc gcgtagcccc tcttacaaaa gggtatacga ggcgatgtct
     3061 cagcgatata aactctatcg tcgaaccagc ggcatttatg ttgtccgcat tagcgttccc
     3121 caacgttttc gtcgatacgc aggacagtgt gagattcaca cttcaacagg cactcacgat
     3181 cttcatgaag caaagcagaa gtccgcgctc ctgttggcgg tctggtatca gaccttacaa
     3241 gagtatgaac aattggatta tcgaacttta agtgactgcg ccccactgct tgctggtgag
     3301 ggtatgatct cgctttctaa ctttgctcaa tcaatcgagt tgcctatatc gcaattgatt
     3361 cgagaggtgg ttaatcgtaa cctcccggta ttctggctgg cgactggtca gttcggtttc
     3421 tatgttgatg aatttaatgc agtagagcgg gaaccgggtg caaaacgaga aaaacagtct
     3481 gatgatgaaa aggatcaacc taaagaagtc atcattctca atagcgcgtt tgagctgggt
     3541 atcgagagct tcgcaaatgg ttatctccgc cccttcaatc cccggcatac tttagattgt
     3601 ctgttgagcg ctggagtatc cgaaggagag gctgcatttc gaactagtgg tgataaccaa
     3661 agtggaggtt ggttcttcga tttacccggc gtagatataa ctgctgatag cctgttgatt
     3721 agcaaagttc atgctgaagg ccttcgactt acatggctgg ttaagaccac gccaccagca
     3781 gttagcattc accctgccgt gcctcttgtc gctcctgtta tcgctaatga atatgttcac
     3841 cgcaaacatt acaatgaaaa cttgtcatgg cttcgtgaag agtatttgaa acatcgacgt
     3901 aagggcaagg tatcagaagc ggcgctccgc gatattcgct attacttcga tttgatgatt
     3961 gaagtgatgg gggatattca gttggaagat ttcgaccgtg atttcctccg ggcttatgag
     4021 agcaagttgc gcacaattcc tgctaaccgt aatttgatga aaggtaagca cggggttaag
     4081 acgctggatg agttaatcgc caaagcggca gaatgtggcg ataaactgat gacagaagag
     4141 tctgtcaaaa agtatatcaa cggcctttat ggtgcaatgg agtgggctgt tgatgatggt
     4201 aagtttctga aatcgccatg cgacaacttt ttccctcccg atgacaaagg tgagcgagag
     4261 caggatcaca ctgacatatt tgaaccgcat gaaattaagg caattttttc gcaaccgtgg
     4321 tttgtcgctg gaactgttga acgtaatgcg caagggcgat tccatcaata ttgcccgttt
     4381 cactattggg cgccgttgtt gggcttgatg acgggggcaa gggttaacga gattgcacag
     4441 ttaatgctgg acgatgttct ggcagatgac ggcgtttatt acctgaacct tgaaagcgat
     4501 agcgaaaacg gaaagaaact aaaaaacgcc aattcccgcc gcaagattcc ggttcattct
     4561 acgctgattg aactcggttt tatcgagtat gtggatgcgt tgaaagctgc cgggtatgac
     4621 cgtctttttc ccgagcttaa accacataaa accaaaggct atggtaggcc ggtttccgca
     4681 tggttcaatg aatcattgct tgcgggtcga ttaaaacttg aaagagacag aagcaaatct
     4741 ttccactctt tccggcattc tgtttcaact ttgcttaaag agaagggtgt tagttcggaa
     4801 ctgcgtgggc agctacttgg gcatgtgcga ggcaaaacag aaactgaagt gcgatacagc
     4861 aaagatttaa aaccggttca catggttgag gttgtcgaaa agattgattt ttctttgccc
     4921 gagatagcga gattcaacat tcctgatggg ctggatgctg taagagatgc gctgcgaaga
     4981 aagcgtggca aacaaacagg ttgagtcagt tactattcag ttgagttatg ctcttgaatg
     5041 cgctgggacg cttcctggcg cattttttat taaaggtcgt ggatttaata gtgccaaatt
     5101 tggagacgct taaaatgcgt ctggtgagac ttgatttgca ttagct
//
LOCUS       DAZKIF010000222         5135 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZKIF010000222 DAZKIF010000000
VERSION     DAZKIF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.224"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..379)
                     /locus_tag="ACHOTW_004564"
     CDS             complement(<1..379)
                     /locus_tag="ACHOTW_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846526.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLD"
     gene            complement(436..831)
                     /locus_tag="ACHOTW_004565"
     CDS             complement(436..831)
                     /locus_tag="ACHOTW_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9846527.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(847..1616)
                     /locus_tag="ACHOTW_004566"
                     /pseudo
     CDS             complement(847..1616)
                     /locus_tag="ACHOTW_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1642..2382)
                     /locus_tag="ACHOTW_004567"
     CDS             complement(1642..2382)
                     /locus_tag="ACHOTW_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9846528.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            complement(2389..3351)
                     /locus_tag="ACHOTW_004568"
     CDS             complement(2389..3351)
                     /locus_tag="ACHOTW_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9846529.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(3374..3799)
                     /locus_tag="ACHOTW_004569"
     CDS             complement(3374..3799)
                     /locus_tag="ACHOTW_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9846530.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(3796..4098)
                     /locus_tag="ACHOTW_004570"
     CDS             complement(3796..4098)
                     /locus_tag="ACHOTW_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9846531.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            4196..4567
                     /locus_tag="ACHOTW_004571"
     CDS             4196..4567
                     /locus_tag="ACHOTW_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846532.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            4588..4779
                     /locus_tag="ACHOTW_004572"
     CDS             4588..4779
                     /locus_tag="ACHOTW_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFP9846533.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            4781..5059
                     /locus_tag="ACHOTW_004573"
     CDS             4781..5059
                     /locus_tag="ACHOTW_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9846534.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT         1227 a         1430 c         1139 g         1339 t
ORIGIN      
        1 catccagagt taccggatag ttggttgacg tttccgagat ttcccgaaaa ttattggttg
       61 acgaaccctt tttttcccga aagtttccag cctgaagcat ggcggcgctg tctggcgggg
      121 cagcatatag cggcacgtat atttccggtt ccttatcagc accgggttgc tcttccagtg
      181 agaatgtctt tccggtaaat cgattcatat aaagcacggg ctctgcttcc agcgaagcct
      241 gagcaacaag ggccagtgct aaatccaact caattgcctc gagagaattt ttgaatgctg
      301 tctgttttac tgcaaatttc atcgccttta cattttcact aacatgactg attaactgct
      361 cttttgtaaa agtggtcatc tcattctcct ttgatgcgaa tgccagcgac aattgaagcc
      421 tgatagctaa ttcactcaca gtaccgcctc ctgaaaattg ccctgataga acgccagtac
      481 acgcagcata acttcactct tccggcactc gccacagatt atgttctgtt gtctgtcgta
      541 gcggcgtatt tctccgtctg gtaactttcg aatcaatgtc tggtcggttg ctttctccgc
      601 tgccttacgc catacgcgat acacctgttc tgatgtaaaa acaccgtatt taccgggcat
      661 gtataaatcg ccacaagcca gtacatccac aaggcaacgt ctgactgaat gccagcctgc
      721 tcccgtcgct ctctccagtt gtgatatcgt catgcgttca tttttgcgta ccagcccgat
      781 aattcgggcc ttcagttctt cacgctgttc gtgtgtaaaa ggtttcgcca taagcgcctc
      841 cggctatcac ttttccgata caacacggcg ggaagaatca gtaatctgtc gaacaatatc
      901 ccggtgcttg ttcagctccc gcagcgcggc gcagactcgc tcccacttct gaacatcact
      961 tttcgccctg cgcagcgcca ggtttgccct gcgcagggac ggaaaaatca gctcatctgc
     1021 ttgcgtttcg gtaaacgatg gcaacggctg cacaatgtcc gccacagttt ctgttttaat
     1081 ttcttcctgt gttgcggctt cccggactgg taacgcagca cctgctggct gaggaaaggc
     1141 cttaccatca tttttcgtta ccggcgcggc tttcggatct gctggtaaat taccccccga
     1201 catgcagtaa cgaaatttac cgttctgatt aacgcgtgcc agccgccccg ttgcggttac
     1261 caccgccagc gtggaagcaa ccttgcgagt actgacgccg aacttacccg ccagttcctc
     1321 acacgtttta gcaccatcct gaccgataaa ctcaaccatc atgtctgcgg taacttttgg
     1381 agcgacctct tcggttacca catccggcgc ttcaggttgt agtgcctgcc cttcggttac
     1441 cccggcttca ccttcgacag ccagaaacca ggtgtgaccc gttttatcaa caacgccatt
     1501 tttttgagtt cccacagttc gttaagaact tcttcacggc tgatatcaag ccgcgccgcc
     1561 agttcaacag aattggcttt tcccatcgct ttcagtgcat gcaatacagt ttccatcgaa
     1621 aatttacctc gtcaaaaatt ctcacatacc ctgacgtcca acgtttgacc gccagctctc
     1681 ccagttaaaa ttcacccaac gaccaccatt catggtcata cggtccatca cccgatctcc
     1741 gaggagtgtg ctcatcgctg cgtgattcag gttcgtcagc attccgacac tacgcatcga
     1801 agccgttctg cggtcgacta tctggttcag cgtgacctgc tcgttgcgcg tatcccgctg
     1861 catgccaatt tcatcaagga ccagaaggtc aactccacaa agctcctgta aaaatttttc
     1921 cccggactgg ccgttgtcgt agccgtcatg caacacgctc atgacatcgg acacggtgac
     1981 gataatcacg cttctcccct tcgccatcag ccgattgcca atcgctgctg ccaggtgatt
     2041 tttaccggta ccaggtttac cgctgaacac gaagtttgta catccggtca tcaattcatc
     2101 ggcaatggat ttcgcctggc tcagagcatg gcgctgaccg tcgttctgca cccggtagtt
     2161 cccgaatgag cacttcctgt gaagcggctg gatgcccgca cggttcagga ttttttcaac
     2221 ccgcacctga tgattcaggc ggttaatctc ctcgctgcgt tttcgtcctt cagcaagttg
     2281 ccattcccgc cactcctcca ccgtccggta cggtggaacc gccccctgtg gtgcaagtct
     2341 gcgaatacgt tcaagaaccc caactgccgc aatgtttttc atgacacgtc accccctgaa
     2401 tcccggcggt atttcagtgt ccggttcaga aatgtgattc acgcaacgct gcgcgggcga
     2461 acgccccagg cggataacca gttcatccca tttttcccgg agttttgccg gactcatgat
     2521 gttttttacc cagaacgaat cccgctggag acgcccaaac atttcacaaa tttgtctgtg
     2581 agttctgcca tccagcatcc gcattgtccg cacgtcattg gcccatgcag tccagttggg
     2641 ttctttcggt ctcgtgatct cgccatcatc gctggccgcc tgctcgtaaa gactcacgat
     2701 tcgtccccag atccactgtg cgcacaccaa atcttcctga cttccccact ggcgtttttt
     2761 cgcactgaac acaaccgcgt cagggtgtcg ggttaaaaaa ttctgttcag ccgtctgcgg
     2821 gtccggttgc gaagcgtccg gacaagaaga tcttttatct gacggatcag gttttaatac
     2881 tgacggatcg gggtcaatca tcgcccccct aatcggcagt ttgttatcaa cagttgatcc
     2941 atcaaaattt gacgggtcaa ccgttgaggg ggcaatattt gacgggtcaa ctgttaacgg
     3001 gtcatttttt gccgggctaa tttttctttt cggtttatat gactcacgcg ccgccgcagc
     3061 agctgcttcg agtttttcca cattaagccg atagatattg cttacattac gcccaccgac
     3121 cttacgctct tccttcgtca gccagccctc tttcgccagt tctgcaatag ccgatttcac
     3181 tgtggattca cttcttgcac cgatctgacg ccggatagtt tcaatggcag gccatgacac
     3241 gccctcgtca ttgctgtagt ctgcaagacg ggccataacc gccaccctgg ataagatcat
     3301 gccggtgaag gcgcaccctt cccagacaag accatgaagc ttgctgctca taaaaccccc
     3361 gaacaccgtg cttttagtgc atcaccacag cattccctgc cgggccgccg cgattcatct
     3421 ggtcatacaa aacaaccgct gacgcaacaa aatcatcgac atccttcacc agccgatccc
     3481 tccgttcgac gatctcacgg taatattcag aactgtggct gcgcatacgg gccaccagca
     3541 aaggcggcat cgccttttcg atcgccggta acagagcctg cattttttca acagcatcag
     3601 gggtgtcttt atccagccaa cggaaaattt tctgggtatt acgggccagg gcttccggat
     3661 ggctgtcgtc atacagttcc gggaacgtca ttcccagctc gaaatacgct ttggtaattt
     3721 tcgcagccgg tactttttcg ccgtccggat gcgcccagac attcatcgcc atgcggatgt
     3781 gttcatgctt gattttcatg aatcaactcc atcagataag catgcactac aatcaccttc
     3841 agcatgaact acatgtgttt gccccaaacg aatgccgctc gcatactcag gccaaataag
     3901 ctcccaatca tggggtcgta gctccgccct acttacttgg ccttccgtcg cagattcgat
     3961 cataagggcg cgggttggag atatagctgt tcgtccagac gccatttgcg ataagtaaga
     4021 tggcgataca ccaagtctgg ccgcgaattt cttagcatca ccaaccctta atgatttaat
     4081 aaactctttt aatgtcatac cttcctcggt ttagtgtttt tttgcgagtt tagtgtttaa
     4141 taaaccatta agtcaagtat ttgcttgttt agtgattact aaagataatt accacatgca
     4201 aaaaaaagaa attcgccgtt tacgtctcaa ggagtggttt aaagataaaa ctctgccacc
     4261 caaagagaag agctacctat ctcaactaat gagtgggaga gcctcgtttg gagaaaaggc
     4321 tgccagaaga atagagcaaa catacgggat gccggaaggg tatctggatg cggaatacgc
     4381 agaacaaccg gggggttctc caccacatgc agggttaacg tctaatcaac tggaattatt
     4441 gcagattttt tcagccttcc ctgaggatga gcaacgccag ataatcagcg agttaaagca
     4501 gaaaaaagaa tcaatggaag atctcatagc gagatggatt gcggcgcaaa aatgccgccg
     4561 cgcctgagtt ataaaaccgg aggaaacatg aatagagccc tttcaccaat ggtttctgaa
     4621 tttgaaacca ttgaacaaga aaacagttac aacgaatggc tgcgtgcgaa agtagcaacg
     4681 agccttgcag atccgcgccc agcaattccc catgacgaag ttgagcgcag aatggcagaa
     4741 cgctttgcta agatgcgcaa ggaacggagc aagcagtaaa atgttacccg tgttatggct
     4801 tgaaagcgca gataccgacc tagatgatat aactagttat attgctcgtt tcgacataga
     4861 tgcggctgaa cgcttatggc agcgattaag gggttgtgtg ctgccgttat ccgaacatcc
     4921 gtatttatac ccaccaagcg acagagtacc tggcttgcgt gagattgtag cccaccctaa
     4981 ctatataatt ctataccgcg taacaacatc aagcgttgaa gtagtaaacg tgatccacgc
     5041 aagacgccag tttccctaac tttcactacc aatagaaaca taacaaccgc aacgacttta
     5101 tcaaaagcgt tgtgtttgtt atgtcccgcg gttta
//
LOCUS       DAZKIF010000223         5052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZKIF010000223 DAZKIF010000000
VERSION     DAZKIF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.225"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..384
                     /locus_tag="ACHOTW_004574"
     CDS             <1..384
                     /locus_tag="ACHOTW_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9846535.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="ACHOTW_004575"
     CDS             419..1117
                     /locus_tag="ACHOTW_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9846536.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="ACHOTW_004576"
     CDS             1139..1363
                     /locus_tag="ACHOTW_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846537.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="ACHOTW_004577"
     CDS             1360..1716
                     /locus_tag="ACHOTW_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HFP9846538.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="ACHOTW_004578"
     CDS             1749..1901
                     /locus_tag="ACHOTW_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846539.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="ACHOTW_004579"
     CDS             1898..2209
                     /locus_tag="ACHOTW_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846540.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2908
                     /locus_tag="ACHOTW_004580"
     CDS             2336..2908
                     /locus_tag="ACHOTW_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846541.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASLASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQ
                     AGNFRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGS
                     CLDEAEQELIVTINEYASQIAVEATQGENQ"
     gene            3018..3122
                     /locus_tag="ACHOTW_004581"
     CDS             3018..3122
                     /locus_tag="ACHOTW_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846542.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3366..3521
                     /gene="hokD"
                     /locus_tag="ACHOTW_004582"
     CDS             3366..3521
                     /gene="hokD"
                     /locus_tag="ACHOTW_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9846543.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3689..3967
                     /locus_tag="ACHOTW_004583"
     CDS             3689..3967
                     /locus_tag="ACHOTW_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846544.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3969..5018
                     /locus_tag="ACHOTW_004584"
     CDS             3969..5018
                     /locus_tag="ACHOTW_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9846545.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRCRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
BASE COUNT         1357 a         1135 c         1409 g         1151 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggccacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg ggggtaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc ctgatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcactgg catcagtatc ggatgaacgg gcagcctatg aattatttat
     2521 ggagaagcgt ttcggggaat ctgtagatcg ccgcagagca aaaaatggcg atagagaata
     2581 catggcatgg gatatggcgc ttggctggat tatctggtgt caccgcgccg ccatgcttca
     2641 ggctggaaac tttcgggaaa ataagggttc gtcaaccaat aattttcgga taatctcgga
     2701 aacgtcaacc aactctccgg caatccctga tgaggtgttg tccgcaatcc tgaaggtcgc
     2761 caggcttcgt gcagatttcg atgcttctga agtggacagg cgaggtatcg gtagttgtct
     2821 ggatgaggcc gagcaagaac ttatcgttac cattaacgaa tacgccagtc agatcgcagt
     2881 agaagcgaca cagggggaga accaatgagc cggccagatg catttgcagg cgttgctata
     2941 gctattgctt ttctagtata tgttatttgt cggtgggggt aaaaacgttc gccgggattc
     3001 acaccaaggg agggaatatg tcggatgata tttcactggt aatggaaggc gctctggctg
     3061 ttattgctgt tgtgggtgtt tactgcctgg ttgtgttttt gatggagcgc ctggggaact
     3121 gaattcattc cgtatgggaa ttcccatatc gggcaaaacg gtttgctgta aagcgagagt
     3181 taagtagaat tgctgcgggt gcttgaggct gtctgcctcg ggcatgccac cgtaaggcag
     3241 acagagaaaa gccccagtta acattacgcg tcctgcaaga cgcttaacat taatctgagg
     3301 cccaatctat gcttcacaaa cgtaggttag cctcttacgc gccgaaaggc aaggagaagc
     3361 aggttatgaa gcagcaaaag gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca
     3421 tagtgacggc actggtaacg aggaaagacc tctgcgaggt acgaatccga accggccaga
     3481 cggaggtcgc tgtcttcaca gcttacgaac ctgaggagta agagaccagg cgggggagaa
     3541 atccctcgcc accgctgatg tgtcaggcat cctcaacgca cccgcactta acccgcttcg
     3601 gcgggttttg ttttttctgg tcgttctggt ttacaatcca tccgtcagcc tgaacaactg
     3661 gcacctgctg cgccagcaga gaaaacagat ggcgcacgat accaaatttt acaattcgga
     3721 taactctgcc gcccctgcca gcaggcacgg gcggcgttct catgcattca aatctgactg
     3781 gtatcagcac gacccctgca ccgaagaaca ggctgaatgg ctgattcagt gttaccgcag
     3841 gcgcggatac gaggttaaga aagccctcag tctcgattat cgtcactgga taatctccgt
     3901 caggcttcct tactccgaac gcccaccgcg tccgtcccgc acattccagc aacgcatctg
     3961 gaggtaacgt gcgggtatta cttcgacctg ttctggtacc ggaactcggg ctggtgatcg
     4021 ttaagccggg ccgtgaatcc atgccggtat tccacaatac ccgggtactg gtggagccgg
     4081 aaccgaaaag catgcgtaat ctgccgtccg gggtcgttcc tgccgttcgc cagccgctgg
     4141 cggaggataa atcattactg ccatttttca gcgacgaacg agtgattcgt gctgctggtg
     4201 gcgctggcgc actgtctgac tggttactgc gccatgttaa atcctgccag tggccacacg
     4261 gcgattatca ccacagtgaa accgtcattc accgttatgg taccggcgca atggtgttgt
     4321 gctggcactg cgacaaccag ttgcgtgacc agacctccga atcacttgag caacttgctc
     4381 accaaaacct gtcagcatgg atgattgacg tcattcgtca cgcaatgaat ggcacacagg
     4441 agcgtgaatt atcgctggct gaattatcct ggtgggcggc ctgcaatcag gtggtggatg
     4501 cactacctga ggcagtagcg cgtcgttcgc tgggattacc agcggaaaaa atccgctccg
     4561 tataccgtga gagtgacatc gtaccgggag aacagacagc catcagcata ctgaagcagc
     4621 gcacaaaaaa tattgcgctg ccacttcacg tccaccagca acaaaatcca ccacagaaaa
     4681 aaacggttgt cagtatcgcc gttgatccgg agtctcctga atcgttcatg aggcggccta
     4741 aacgttgccg ctgggttaat gagaaataca cgcgctgggt aaagacacag ccgtgtgcgt
     4801 gttgtggtaa gcctgctgac gatccgcatc acctgattgg tcatggtcag gggggaatgg
     4861 ggacaaaggc ccacgatatt ttcacgctac cgttgtgccg ggagcaccac aacgaacttc
     4921 atgcagaccc gctggagttt gagaaaaagt acggctctca gattgagtta atttttcgtt
     4981 ttcttgatca cgcctttgcg actggcgtgc tcgggtaaaa gaggtgactg atgctcatag
     5041 atttggtttt ac
//
LOCUS       DAZKIF010000224         4998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZKIF010000224 DAZKIF010000000
VERSION     DAZKIF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.226"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            50..319
                     /locus_tag="ACHOTW_004585"
     CDS             50..319
                     /locus_tag="ACHOTW_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9846546.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHGQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            605..676
                     /locus_tag="ACHOTW_004586"
                     /pseudo
     CDS             605..676
                     /locus_tag="ACHOTW_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(687..935)
                     /locus_tag="ACHOTW_004587"
     CDS             complement(687..935)
                     /locus_tag="ACHOTW_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9846547.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(1163..1228)
                     /locus_tag="ACHOTW_004588"
                     /pseudo
     CDS             complement(1163..1228)
                     /locus_tag="ACHOTW_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1450..3135
                     /gene="btsS"
                     /locus_tag="ACHOTW_004589"
     CDS             1450..3135
                     /gene="btsS"
                     /locus_tag="ACHOTW_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP9846548.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3132..3851
                     /gene="btsR"
                     /locus_tag="ACHOTW_004590"
                     /gene_synonym="yehT"
     CDS             3132..3851
                     /gene="btsR"
                     /locus_tag="ACHOTW_004590"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP9846549.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            3898..4368
                     /gene="yehS"
                     /locus_tag="ACHOTW_004591"
     CDS             3898..4368
                     /gene="yehS"
                     /locus_tag="ACHOTW_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP9846550.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(4410..4871)
                     /gene="yehR"
                     /locus_tag="ACHOTW_004592"
     CDS             complement(4410..4871)
                     /gene="yehR"
                     /locus_tag="ACHOTW_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP9846551.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
BASE COUNT         1255 a         1111 c         1297 g         1335 t
ORIGIN      
        1 aaggggctgg atgaagtggg cagtgataca ggcagaacag gagaatgaca tgaatatact
       61 aaaaaaactt atgcagcgtc tgtgtggttg cggaaagcat gatggccgtg aacacgggca
      121 gtcgcttaca gcacaactgc gactggggcc ggcagacatc ctggagtccg atgagaatgg
      181 tattattccg gagcaggaca gggtaatcac gcaggtggtg atactggatg cggataaaaa
      241 gcagatacag tgcgtggtaa gaccgctgca aatcctgcgt gctgacggga cgtgggaaaa
      301 tattggcggg atgaagtaac ccgacagctt cacaaaaccg gagtccggct ccggtttttg
      361 tgttgcaatg tccgggggat atttgttagg tagattgtat tgtatgtaaa ggcagagctc
      421 cgaattttac tgttgtaagt gggcctggcg ggaatggtat cgccagcatc attggcttgg
      481 gtccagtaaa aagagacgca gaaagatgca caataggcac tacgcgtcat gcatggatta
      541 ggtcgctcat tatttacaca gtactgctaa aaaattatta aggaaagcgc ggcacttcgt
      601 atgtaagagc gtgtcgcggt tggctggtga actttcgata gtgcgagtat tgaatgattt
      661 ccagccgtta ccgatttacg tgtttattag cgaacaaacc actcgtcagc agactcccag
      721 gtatctttca gcgtctcctg aacaaaagtt ttagctgaat ctttatcggc ggtgcgcgta
      781 acagaaaggc catcgttgct ggtggctttt acgatcacct ctacatcgtc ataacgttta
      841 ctgatgcgtc gggttaattc ttcctttaac gcatccacag caccgtttgg cattttagtg
      901 attttttctt tagcgataca gatctcaaca cgcataacac cctcctgtaa ctgtagttat
      961 gtacaggtgt tatttttatc tgtatggata accagtgtca attcctgata ttgtttatgg
     1021 ggcatcaatg gggcatgtat gggacacttt ttatcggcga aattcgtcga agttcatcga
     1081 catggtaaac gaatcatcta tccaaccctt gaaaaacggc gctcctggac gatcttcgtc
     1141 gatttttaaa aatgttgcgt cacgcgcgta acgtgacagg gttaatatca caaagcaacg
     1201 ccacttcacc aattgtgtaa agcgccatcg tctcaccctt gctcgcgagg tcccggttta
     1261 actttagacg cagttttgcg aaccaggtag ttttgcccgt tttttgtgca tttatagggt
     1321 gattttattt ttgccaggcg attttgagtg atcgtactca cgaattctca tttttctgca
     1381 agagttcaaa gaaagttaaa cgcaggcaat gtatgttacg cgttttaaag ggatgtgtgg
     1441 tttgcgggta tgtacgattt taatctggtg ttgctgctgc ttcagcagat gtgcgttttt
     1501 ttagtcattg cgtggttaat gagtaaaacg ccattattca taccgttaat gcaggtcacg
     1561 gttcgtctgc cgcataaatt tctctgctac atcgtctttt ccatcttctg catcatgggc
     1621 acctggtttg ggttgcacat tgacgattct attgccaata cccgtgcgat aggcgcggta
     1681 atgggcggct tactcggcgg tccggtcgtc ggtgggctgg ttggcctgac cggcgggtta
     1741 catcgatatt cgatgggggg catgaccgcg ctaagttgca tgatctcgac catcgttgaa
     1801 ggattgctcg gcggcctggt acacagcatc ctgatccgtc gcgggcgcac tgataaagtc
     1861 tttaacccca ttaccgctgg tgccgtcacg ttcgtcgctg aaatggtgca aatgctgatc
     1921 atcctggcga tcgcccgacc ttatgaagat gcggtgcgtc tggtgagtaa tattgctgcg
     1981 ccaatgatgg tcaccaatac cgtcggcgcg gcgctgttta tgcgtatatt gctcgataaa
     2041 cgcgcgatgt ttgaaaaata cacttcggct ttttctgcca ctgcgctgaa agtggcagcc
     2101 tcgacggaag gcattttgcg ccaggggttt aacgaagtga acagcatgaa agtggctcag
     2161 gtgctgtatc tggagctgga tattggtgca gtcgcgatta ccgatcgaga gaaattgctg
     2221 gcctttaccg gaattggtga cgaccaccat ttacccggca aaccgatttc ttcaacttat
     2281 actttaaaag cgattgaaac cggtgaagtg gtctacgctg atggcaacga agtaccttat
     2341 cgttgctctt tgcatccgca atgcaaactg gggtcgacgc tggtaattcc gttgcgtggt
     2401 gaaaatcagc gggtgatggg caccatcaaa ttgtatgaag ccaaaaaccg tttatttagt
     2461 tcaatcaacc gcacgctggg cgaggggatt gcgcaactgc tttcggcgca gatcctcgcc
     2521 gggcaatatg agcggcaaaa agcgatgctc acccagtcag agatcaaact gcttcacgcc
     2581 caggtgaacc cccatttttt gtttaatgcg cttaacacca ttaaagcggt gatccgtcgc
     2641 gacagcgaac aggccagcca gctggtgcag tatctttcca cttttttccg caaaaactta
     2701 aagcggcctt cggagtttgt tactctcgcc gacgaaattg aacatgtgaa tgcttatctg
     2761 caaattgaaa aggcgcgctt ccagtcgcgg ttgcaggtca acattgctat tccgcaagaa
     2821 ttatcccagc agcaattgcc cgcgtttacc ctgcaaccga tagtggaaaa cgccattaaa
     2881 catgggacat cacaactgct ggatacaggg cgagtggcaa tcagcgtccg acgtgagggg
     2941 caacatttga tgctggagat cgaagacaat gccggtttgt atcaaccggt aaccaatgcc
     3001 agtgggctgg ggatgaatct ggtggataag cgtttacgtg aacggtttgg cgatgactat
     3061 gggataagcg tcgcctgtga gcctgatagt tacacccgaa taacgttacg actaccatgg
     3121 agggacgagg catgattaaa gtcttaattg tcgatgatga accgttagca cgggagaacc
     3181 tgcgcgtatt tttgcaggag cagagcgata ttgaaatcgt tggtgagtgt tcaaacgccg
     3241 tggaagggat cggcgcggtg cataaactgc gcccggatgt gctgtttctc gatatccaga
     3301 tgccgcgcat cagtggtctg gaaatggtgg ggatgctcga cccggaacat cgtccgtata
     3361 ttgtttttct caccgcgttt gacgaatacg ctattaaagc ctttgaagaa catgcctttg
     3421 actatctgct gaagccaatt gatgaagcgc gactggagaa aacgctggcg cgattgcgtc
     3481 aggagcgcag caagcaggat gtttcgctgt taccggaaaa tcaacaggcg ctgaaattta
     3541 tcccttgtac ggggcatagt cggatttatt tgctgcaaat gaaagatgtg gcatttgtca
     3601 gcagtcggat gagcggtgtc tacgttacca gccacgaagg gaaagagggc tttaccgaat
     3661 tgacattacg taccctggaa agtcgtacac cactactgcg ctgccatcgt cagtatctgg
     3721 ttaacctcgc gcatttacag gagattcgtc tggaagataa cggtcaggcc gagttgattt
     3781 tgcgtaatgg cttaaccgtg ccggtcagcc gccgttatct gaaaagctta aaagaggcga
     3841 ttggcctgta aaagactgct aaaatggctt tttgcctcat caacacctga aggcctcatg
     3901 ctaagtaacg atattctgcg cagcgtgcgc tacattttga aagccaataa taatgacctg
     3961 gtgcgtatac tggcgctggg taatgtcgaa gccaccgcgg aacagatcgc cgtctggcta
     4021 cgtaaagaag acgaagaggg ttttcagcgt tgtccggaca ttgttttgtc gtcattcctc
     4081 aatggcctga tttatgaaaa acgcggcaag gatgagtctg ctccggcact ggagccggaa
     4141 cgtcgcatta ataacaacat cgtgctgaaa aaattacgca tcgcgttttc gctgaaaacc
     4201 gatgacattc tggcgatcct caccgaacag cagttccgcg tttcgatgcc ggaaattacg
     4261 gcgatgatgc gtgcaccgga tcataaaaac ttccgcgaat gcggcgatca atttttacgt
     4321 tattttctgc gtggactggc agcgcgccag catgtgaaga aaagctaaga cgggtatggc
     4381 ggccatgcgc aaacatggcc gccgacagat tatttcactt ctttaaaacc agcggctttc
     4441 atcaccagtt ccatttgcgc catagtgata ccttttttgg catcttcagc agaaacgttg
     4501 attcctgaaa taccctgcag ggctttaaaa tccacttttt ccatatcgat agtcacgttt
     4561 tcctgcgcgt aggtatcggt ataggttaat ttttcttcaa cacccgcgat gtttttgtat
     4621 ttggcgctta acggctcaag tgtcttggca gcatcttctt tggtggtagc accaatggag
     4681 gcaaattgaa ttttggtttc agaagattgc ttaagcacct tgtcaccttt gtagacatag
     4741 gtaatggcaa tttcagtgcc gttcagattg gcgctgaatt tcttcgattc ttctttgtca
     4801 ccgcagccag caagagaaaa aaccagaaca gatgcaacaa caagggaaaa cagcttattg
     4861 aaagccttca tgtaaaactc cattttattt aatcaaggaa ctggtgactc tcaccagggg
     4921 ctatatagga tatgcctaat actgtggcgt gagcagtccg gaactggagt agaactctta
     4981 gtaaaaagca ctatttca
//
LOCUS       DAZKIF010000225         4986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZKIF010000225 DAZKIF010000000
VERSION     DAZKIF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.227"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..926
                     /locus_tag="ACHOTW_004593"
     CDS             <1..926
                     /locus_tag="ACHOTW_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFP9846552.1"
                     /translation="LSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLN
                     LTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGS
                     YDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIID
                     NMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEGQNFSGLR
                     QRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLN
                     EEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1135..2100
                     /gene="lipA"
                     /locus_tag="ACHOTW_004594"
     CDS             1135..2100
                     /gene="lipA"
                     /locus_tag="ACHOTW_004594"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP9846553.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2201..2404)
                     /gene="tatE"
                     /locus_tag="ACHOTW_004595"
     CDS             complement(2201..2404)
                     /gene="tatE"
                     /locus_tag="ACHOTW_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP9846554.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2533..3321)
                     /locus_tag="ACHOTW_004596"
     CDS             complement(2533..3321)
                     /locus_tag="ACHOTW_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP9846555.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3414..3797
                     /gene="crcB"
                     /locus_tag="ACHOTW_004597"
     CDS             3414..3797
                     /gene="crcB"
                     /locus_tag="ACHOTW_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP9846556.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3851..4060)
                     /gene="cspE"
                     /locus_tag="ACHOTW_004598"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3851..4060)
                     /gene="cspE"
                     /locus_tag="ACHOTW_004598"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9846557.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4235..4795)
                     /gene="pagP"
                     /locus_tag="ACHOTW_004599"
     CDS             complement(4235..4795)
                     /gene="pagP"
                     /locus_tag="ACHOTW_004599"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP9846558.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1336 a         1258 c         1106 g         1286 t
ORIGIN      
        1 gtttaagcag aaaatcgtca gaaggtaaac cacaaatatt tacaacctta cgaaatattg
       61 atcttaatct tctgactatt tttgaagctg tatatgtaca taaagggatc gttaatgcag
      121 cgaaagtgct taatctgacc ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta
      181 tattccctga cccattgttt attcgcaaag gccagggtgt cactcctacc gcatttgcga
      241 tgcatctaca tgagtatatc agtcagggcc ttgagtccat tcttggcgcg ctggatatcg
      301 aaggaagcta tgataagcaa cgaacgataa ctattgcgac caccccctcg gtcggagctc
      361 tggtccttcc tgtcatttat cgggcgatta aaactcacta tccgcagctt ttactgcgca
      421 acccgcccat cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatag
      481 ataacatgtt ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc gacaatatgg
      541 tgttaatttg ccgcgaggga aatccactac tctctttaga agatgacaga gagactatcg
      601 acaacgctgc acatgtactc ctgttaccgg aaggacaaaa tttcagcggt ctgcggcaga
      661 gaattcaaga gatgtttccg gaccggcaga tcagcttcac cagctacaac attttgacaa
      721 tcgctgcact ggttgccaac agtgacatgt tagcgattat tcccagccgt ttttataacc
      781 tgtttagccg ttgctggccg ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa
      841 tagatttctc tatccactac aacaaattca gcctgcgtga tcccatcctg cacggagtaa
      901 tagatgttat ccgtaatgca ttttgaaaaa gtattgttac agccagatca cagcgcagaa
      961 cgataaaatg gcacaatcca acaacaattt tacattttcg cgaccgcttt ggctgctttc
     1021 aggtccgttt caatgatata ctgccagtcg ttaattcaaa aatagttgat aattacaaca
     1081 atctattgaa ttgaaacgct ttccttcgta attcgcaact ggaacacgca cgctatgagt
     1141 aaacccattg tgatggaacg cggtgttaaa taccgcgatg ccgataagat ggcccttatc
     1201 ccggttaaaa acgtggcaac agagcgcgaa gccctgctgc gcaagccgga atggatgaaa
     1261 atcaagcttc cagcggactc tacacgtatc cagggcatca aagccgcaat gcgcaaaaat
     1321 ggcctgcatt ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg cttcaaccac
     1381 ggcacagcaa cgtttatgat cctgggcgct atttgtaccc gccgttgtcc gttctgtgac
     1441 gttgcccacg gtcgcccggt agctcctgat gccaatgagc cagtgaaact ggcgcagacc
     1501 attgccgata tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc
     1561 gatggcggtg cccaacactt tgcggattgt attactgcca ttcgggaaaa aagcccgcaa
     1621 atcaaaattg aaactctggt gccggatttc cgcggtcgta tggatcgtgc tctggatatt
     1681 ctgactgcaa cgcccccaga tgtgttcaac cataacctgg aaaacgtacc gcgtatttac
     1741 cggcaggtac ggcctggcgc agattacaac tggtcgctga agctgctgga acgctttaaa
     1801 gaagcgcatc cggaaatccc gaccaagtct ggtctgatgg tgggtctggg tgaaaccaat
     1861 gaagaaatta ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg
     1921 gggcaatatt tgcagccaag ccgccatcac ctgccggttc aacgttacgt tagcccggat
     1981 gagttcgacg aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt
     2041 ccgtttgtcc gctcttctta ccacgccgat ttgcaggcga aagggatgga agttaagtaa
     2101 tcctgtaata attacttaca tctgtctgat aaaaaacccg ctttttgaag cgggtttttt
     2161 gtatcgaaca agatattgag ggagcgtcct gctcgccacg tcactcttta tgagagagct
     2221 tttcagcctg aagatcaacg tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg
     2281 ccttcttgaa ccctttaatg gccgctccaa ggtctccgcc cagcgtacgt aacttcttag
     2341 tcccaaacag cagaacgacc agcgccgcaa ctaccagcag tttggtaata ctaatctcac
     2401 ccatagatac cttcttgaca tataaccggc aacagaacgc gccttaaaaa ccaaagaata
     2461 ttaacggtca ttcgacgcgc gcacacaata gcaatctgta acaaggtgaa tcaagcgccg
     2521 agtaaaaaaa catcataata attgcggcgg cgcaaagcga cggttgttta agacgggcag
     2581 ttgcgcgcgc acctgacgca cacgttcggg cgtcacttcc gccataatga gtgcaggcat
     2641 ttctgacgct gccgcaatgg tgacgccaaa gggatctata atccggcttt gcccgatatt
     2701 tttgttcccg cactcccccg ccgccaccat ataacaggtg gtatccagcg cacgagcggc
     2761 aagcaacgtt gaccagtggt gctctttgag cggcccgcga acccaggcgg caggaagtac
     2821 caggatttca gctccctgta atgcctgtgc cagcgccagc tctggaaagc gtaagtcata
     2881 acaggtcatc agaccgacct tcatcccctc cacctcgagt aacggagcaa tttcattacc
     2941 agcatcaaca cggcgtgatt cctgaatggc aaatgcatca tagagatgca atttggcata
     3001 acgggcgacg atgtttcctg cctgtagcgc caccagcata ttccatgcgc gccccggcgt
     3061 tgaaggaaca tgaatcgtca gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa
     3121 acgtccgagg aattcgcctt ccagcagctg tgctgattta accgatagat ctgcatcatg
     3181 atcatcgcgc gccagcaatg cttccggcag gacaaacagc gatacgtcgt tttccgccgc
     3241 ctgcgccatc aacgaggcac aaatctcagc gttcttttcc cacacagatg taacagcaaa
     3301 ctgtcctgca gcaaccagca ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg
     3361 attattcggc gttacactta tcactcatac aaatcaaata gcaggatttt gcagtgttac
     3421 aacttctttt agcagttttt attggcggtg gtacgggaag cgtggcgaga tggctgttaa
     3481 gtatgcgatt taacccgctg catcaggcga ttccgttggg gacgctggca gcaaacctga
     3541 ttggggcatt catcatagga atgggattcg cctggttcag caggatgacg aacattgatc
     3601 cagtgtggaa agtattaatc accaccggat tttgtggcgg tctaacaacc ttctcaacat
     3661 tttcggcaga agtggtgttt ttgttacaag agggccgctt tggctgggca ttactgaacg
     3721 ttttcgtcaa ccttctgggg tcttttgcca tgaccgcact ggcattctgg ctgttttcgg
     3781 cctcaaccgc acactaaaga ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg
     3841 ctgacgtatc ttacagagcg attacgtttg cagcagaagg gcctttggca ccgttagtga
     3901 tttcgaactc tacgcgctga ccttcagcaa gagttttaaa accattagtc tggattgcag
     3961 agaagtgtac gaacacgtct ttgctgccgt cttccggagt aatgaaaccg aatcctttgg
     4021 actcattaaa ccacttaacg ttacctttaa tcttagacat caaaattacc tttacatgaa
     4081 aaatagacac aaatgctgtg tcggttacca gtacaccaat tgtggtacgc tttgtccagt
     4141 gcaactttac taaaaagtga taaaaatcgc gttatttttg atgttgctta ttttagttat
     4201 tgattttaaa gaagttacta aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa
     4261 agtacacatt gccattgttg taggtacccg gaatgtaggt catctgaaaa gtaactgggc
     4321 cataacccac ggaggccaat ggcagtagaa ccgggagagg gatgtaattc cagttatcgc
     4381 gtgccgttac gccagcggtg aatcccagac ctaaatgaaa attttcatcc gccagcggtc
     4441 gccaggtact ttcccatccg tatccggcaa tcggttccca tttgttccac gagtccttaa
     4501 atgccatggc atacaggcca tgccagtttc ctttttcatc ccaacgcgac aggccaaaac
     4561 cgccacccca cggtcgctcg ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg
     4621 catgccaggt gatggcagga atatataaat cataatgttc aggctgttgc caggtttgta
     4681 caatattttc tctaaacgtt gtcatccact catctgcgtt agcaaaaact ttaccaacgc
     4741 tgattaactg aataaaaaca aaggaaaaga tagcgacata tttactcacg ttcatttgtg
     4801 accataaaac atttatcaaa aatctactac tagcatagca aagctacaat taacataacc
     4861 ttaacagccc caacataaag aataatctga atagctgctc tatttatttc cgcttgttca
     4921 agttaaatat ttccgcgcat cgatttaaaa tgacacatcg ttttctgcga tgggaatagt
     4981 cataaa
//
LOCUS       DAZKIF010000226         4930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZKIF010000226 DAZKIF010000000
VERSION     DAZKIF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.228"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(67..597)
                     /gene="cybB"
                     /locus_tag="ACHOTW_004600"
     CDS             complement(67..597)
                     /gene="cybB"
                     /locus_tag="ACHOTW_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP9846559.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            786..1787
                     /gene="gap"
                     /locus_tag="ACHOTW_004601"
     CDS             786..1787
                     /gene="gap"
                     /locus_tag="ACHOTW_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9846560.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(1829..3268)
                     /gene="aldA"
                     /locus_tag="ACHOTW_004602"
     CDS             complement(1829..3268)
                     /gene="aldA"
                     /locus_tag="ACHOTW_004602"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP9846561.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3465..4265)
                     /gene="ydcF"
                     /locus_tag="ACHOTW_004603"
     CDS             complement(3465..4265)
                     /gene="ydcF"
                     /locus_tag="ACHOTW_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP9846562.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(4381..4758)
                     /locus_tag="ACHOTW_004604"
     CDS             complement(4381..4758)
                     /locus_tag="ACHOTW_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846563.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEH
                     YVIATSNDVIDVLSTVSPQIVYD"
BASE COUNT         1319 a         1218 c         1108 g         1285 t
ORIGIN      
        1 gtagacccgt attcttcgcc tgtaccacgg gtcggtttta gtacaggcgt tttctttaaa
       61 tatccttcag gaacgtttac gcggcatcat gcgtagaagt gtgttgtcct tccagaaata
      121 gtggtgtgcc agtgcagccg cagcgtgcaa accgatgaca aaatatccca gattcgccag
      181 cgtttcatgc cacgacttta agctatctac ccgttcgaaa ttggcctctg aagcgtaagg
      241 catcgtcaaa ccaaacgcaa accacgggtt gcctcggtta tacatcatca ccaaaccaat
      301 cactggcagc gcaataaaca ggagataaat caccaaatgt cccaaatgcg ccagtcccgt
      361 catcatcggt tttggcttag gtataatcgg cggggttggg tatttcagcc ttaacaaaag
      421 acgaacgacc atcagcacga gaattgatat gccgcaggaa acatgaatca tgttgatgag
      481 tggccgatcg ctacgtggga agaaaccccg aaactccatt gcgcaatacg ctgcgataac
      541 cagcaaaaag accagccagt gaatgctgat ttgtaacctt gagtatttat tttccataac
      601 atttcctgct ttaacataat tttccgttaa cataacgggc ttttctcaaa atttcattaa
      661 atattgttca ccctttttca gtacgttacg tttcactgta agaatggaga ttgcccatcc
      721 cgccatcctg gtctaagcct ggaaaggatc aattttcatc cgaacgttcc tgacaggaga
      781 aaaccatgag taaagttggt attaacggtt ttggtcgtat cggtcgactg gtgttgcgtc
      841 gattacttga agtcaaaagc aacatagacg ttgtcgctat taatgatctt acttccccaa
      901 aaattctcgc ctatctgctg aaacatgatt caaactatgg tccgttcccc tggagcgttg
      961 attttacgga agattcactt atcgttgatg ggaaaagtat cgcggtttac gccgaaaaag
     1021 aggctaaaaa tattccgtgg aaagcgaaag gtgcagaaat cattgtcgaa tgtactggct
     1081 tttatacctc cgccgagaaa tcgcaggcgc atcttgatgc tggcgcgaag aaggtgttga
     1141 tttccgcccc tgcaggtgaa atgaaaacta tcgtttataa agtcaatgac gacactctgg
     1201 atggcaacga caccattgtt tccgtggcat catgcactac taactgtctt gcgccgatgg
     1261 ccaaagcctt gcatgacagt ttcgggatag aagtcggcac gatgacgacc attcatgcct
     1321 atactggcac ccagtcactg gtggatggcc cgcgtggtaa agatttacgt gcttcacgcg
     1381 ccgcggcaga aaatatcatt ccccacacta cgggcgcggc aaaagccatt ggtctggtga
     1441 tcccggaact gagcggcaaa ctgaaaggtc atgcgcaacg cgtgccggtg aaaacaggtt
     1501 cggtcactga actggtatcg attctcggaa aaaaagtgac tgccgaagag gtgaataacg
     1561 cacttaaaca agcaaccacc aataacgagt catttggtta taccgatgaa gaaatagtct
     1621 cttccgatat cattggcagc catttcggtt cggtgtttga tgccacgcaa acggaaatta
     1681 ccgccgtggg cgatttacaa ctggtgaaaa cggtcgcctg gtacgacaac gaatatggct
     1741 tcgtcacgca gcttattcgc accctcgaaa aattcgctaa actctgacgc gcacaggcgg
     1801 aggaaaaaac ctccgcctct ttcactcatt aagactgtaa ataaaccacc tgggtctgca
     1861 gatattcatg caggccatgt ttaccatctg cgccgccaat accggattta cgccatccgg
     1921 cgtggaagcc ttgcatagct tcgaagtttt cacggttgat gtaagtttca ccaaacttca
     1981 gccctttaat ggctttcatc gcgacgttca gattttgggt atagattgat gaggtcaggc
     2041 cgtaatcact gtcattagcc attgagatag catcttccag cgtgtcaaat gcgactaccg
     2101 gcagaaccgg gccaaaggtt tcctcatgca taatcgacat ttcctggcga acatccagca
     2161 gcaatgtcgg cggataataa tatcctttcc cctctaccgc tttgccaccc aacgccactc
     2221 tcgccccttc ttctactgcg cgcgccactt tttgctcgac cctttccagc gccgcggcgt
     2281 taatcaacgg ccccatcgca atgtcgttgc gttcagcggg gttaccaaat tgaaccgcct
     2341 gcatcgcttc acccagccga ttgacgaact gatcataaat gcctttctgt acataaacac
     2401 gttctgcaca gttacacact tgcccactat taatgacgcg tgaatcaacg atggctttga
     2461 ctgccagttc aagatcggca tcgtccatta cgatagctgg tgctttaccc cccagttcca
     2521 ggcacacttt ggtgatgttt ttcgccgcag tcgccatgat cttctcacct gcagagacgc
     2581 tgcctgtcat actgaccatt gcgacctttg ggttacccgc cagttcttgc ccaacggttt
     2641 caccacgccc cagaacaagg ttaaacacgc cgcgcggaag gcctatttca tcgacgattt
     2701 tggcgaatgc aatcgcattg tttggcgtaa attcactggg tttaatgaca atggtattac
     2761 cagtcaaaag agcgggagcc attttgcggg caatgaggaa gaacgggaag ttccacggca
     2821 gaatgccggt agtcacacca agcgcacgtt taaacagaag aatattttct cctggacgat
     2881 cgctttgaat aatctcgccc tcgtaacgcc gtgcccactc cgccatgtaa tcgatatagt
     2941 cggcagtaaa agccacttcg acttcagcca gctgctggat cttgccccct tcttcaacaa
     3001 tcagcgcact gatttcactg gcgcgttcgc ggatcccggc ggagattttg cgcaaccaac
     3061 tggcgcgttc aattgcaggc aacgcttccc attctggttg tgcacgttct gctgcatcga
     3121 ttgccttacg ggcatcctcg gcctgaccat cgggaatgcg ggaaatgaca gcctctgtag
     3181 cagggtttac cacatcaatc catgcgtctc cacgccaggt aacaaactgt ccatcaatat
     3241 acataggatg ttgaacgggt actgacatgg gcgactcctg tgatttatat gtttgttttt
     3301 ggcaaataca ttgttaatta acatcaatca tcatagagac ggcatcatga aaggacaatt
     3361 ctgtgaaggg cttcataaaa acaagtgtaa aaggaataag aggcagaaat aaaaggcagg
     3421 agttatgccc gtttgcaaac aagtggttca caaacgggca atttttaacg taacgagcga
     3481 ctttccatcg cctcgatgag caccgcatcg tgtttcagcg tttgccatgc atggatgact
     3541 tctgccggaa aatcaacgtg aacgataaaa tctcgcccgc ggggaccgta gccatcgcta
     3601 tcatcgcgta aacgcggcag ctcgccagtg agtagtgaga gataacgctc aaccggccat
     3661 aatccttgta actgattaat aaagattaca ctgtctgcgt tatttcctaa ctgaggaacg
     3721 aatccgggat aacttaacca gcgtggtaca tcgggattgt ccccggtcat acggcggaac
     3781 gtcgccatcg tgcgccgctg catggtgggg tcctgtacaa cgatagctgt gtgaactcgt
     3841 tctacggcct gattcaatag cgcgatgcta aagcgtgcgt tttcaccgca gtttgttgac
     3901 tggtcttcaa tccagatttt ttcatgcgga atatgccaga actgatgagc gatatccgcc
     3961 aggatggtcg cttctgctct gccagtggtg cggatagtgt tgtagtgcgg atgctgtgcg
     4021 atggcgctat acaaaaaagt ggtcgagtga ccgataccac cactaatcag taaaggaatt
     4081 tgctgatcgc gagcaatctt acatgccgca tcgatagtcg gcataaccgc atttcctgca
     4141 agtatgacgc aatcagcctg atacggcacc tcaccggaga aatcatcctg cgccagccac
     4201 tgtccgataa catttatggc atctatagtt gccgtcgaaa gcgtcggaaa cggggtaatg
     4261 ttcatggttc ctccttatcg ttcccttaag aatactgcgt gagataatag tgagaagcgg
     4321 agcactctga aaggaagata atgctttttc tatctcgcca tcgcgaacca gacactggtt
     4381 ttagtcatac actatttgcg gagaaactgt tgataaaaca tcaataacat catttgacgt
     4441 ggcaataaca taatgttcga tcattttatt tttatgaatt tcacaacttt gcatagtaaa
     4501 ccatgtaaga taagtggaat tggctactct atagatcccc gtttggttaa ttccctgctt
     4561 tcctgacata cctaacaaat caccttcgtc agtaacacga aatgagtgta tccagtcaaa
     4621 tgatatctta acttctgact tgatcgccgg gcatatcagg gccagttcaa gcctggcatt
     4681 cttatagctt aaacctgaca gatcggcgtg tttaaaagac aacgaagtcg gcgtgatatt
     4741 tataaaacgt tcatccatgt taacaccctt aatatatgag actgtactca atttaacctt
     4801 ctacaaaaat ggtgtgagtc tgtttaagta ccatgtataa tcgataaccg ttgctgaatt
     4861 ttaaaattat cttgtccagt tattaaccat ttttcatggg aaacatctgc aatacattca
     4921 ttaatgcgaa
//
LOCUS       DAZKIF010000227         4930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZKIF010000227 DAZKIF010000000
VERSION     DAZKIF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.229"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..747)
                     /gene="sepL"
                     /locus_tag="ACHOTW_004605"
     CDS             complement(<1..747)
                     /gene="sepL"
                     /locus_tag="ACHOTW_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFP9846564.1"
                     /translation="MANGIEFNQNPASVFNSNSLDFELESQQLTQKNSSNISSPLINL
                     QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGSTIEKLLNDMQELLTLTDSDKIKE
                     LSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYARLLLDLLGE
                     DDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREKH
                     LKVIIRALSFDLSYMSSFEDKVKTSSIISDL"
     gene            890..2110
                     /gene="escD"
                     /locus_tag="ACHOTW_004606"
     CDS             890..2110
                     /gene="escD"
                     /locus_tag="ACHOTW_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFP9846565.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DVLTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNAKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
     gene            complement(2374..>4930)
                     /gene="eae"
                     /locus_tag="ACHOTW_004607"
     CDS             complement(2374..>4930)
                     /gene="eae"
                     /locus_tag="ACHOTW_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFP9846566.1"
                     /translation="LSTIWSLNKHLYSSESEMMKAAPGQQIILPLKKLPFEYSALPLL
                     GSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDDKALNYAAQQAASLGSQLQSRSLNGD
                     YAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQSGNNFDGSSLDFLLPFYDSEKMLAF
                     GQVGARYIDSRFTANLGAGQRFFLPANMLGYNVFIDQDFSGDNTRLGIGGEYWRDYFK
                     SSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNGYLPSYPALGAKLIYEQYYGDNVA
                     LFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGNENDLLYSMQFRYQFDKSWSQ
                     QIEPQYVNELRTLSGSRYDLVQRNNNIILEYKKQDILSLNIPHDINGTEHSTQKIQLI
                     VKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQDYQAILPAYVQGGSNIYKVTARAYD
                     RNGNSSNNVQLTITVLSNGQVVDQVGVTDFTADKTSAKADNADTITYTATVKKNGVAQ
                     ANVPVSFNIVSGTATLGANSAKTDANGKATVTLKSSTPGQVVVSAKTAEMTSALNASA
                     VIFFDQTKASITEIKADKTTAVANGKDAIKYTVKVMKNGQPVNNQSVTFSTNFGMFNG
                     KSQTQATTGNDGRATITLTSSSAGKATVSATVSDGAEVKATEVTFFDELKIDNKVDII
                     GNNVRGELPNIWLQYGQFKLKASGGDGTYSWYSENTSIATVDASGKVTLNGKGSVVIK
                     ATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMSICKNLLPSTQTVLSDIYDSWGAANK
                     YSHYSSMNSITAWIKQTSSEQRSGVSSTYNLITQNPLPGVNVNTPNVYAVCVE"
BASE COUNT         1448 a         1076 c          934 g         1472 t
ORIGIN      
        1 taaatcacta ataattgaag atgtctttac tttgtcttca aaagaagaca tataagagag
       61 atcgaacgac agcgccctaa taatgacttt taagtgcttc tcacgctcgg gaagatcctt
      121 aatctccata aaccagtcaa gtaaagaggc gccattttca gaatcctcat ccttagcctt
      181 ttcataaagc ttcttgatct tctggatacc ttcctgattt aactcaccca cccctaacca
      241 ggaaagcaga gccagctccc aatcatcttc gcctaataaa tctaataata acctggcata
      301 tttctttttt aattcaatct taacaaactt agattgcaat aaagaagata ttagcgcaac
      361 aatttccccc tttggcatgt tgccaaacat cgccaaagta ggatcgtgtt gttccagcag
      421 cccgctgttt tttaatgata gttcttttat tttatcacta tcggtaagag taagcagctc
      481 ttgcatatca ttgagcagct tctcgattgt cgagccttcc tcttctttcc ttgcgctacc
      541 tttgcgatat cccaggctta gcccttcaat cgtttcagaa agcgatgaac tagtaatcat
      601 tgctaattca ttttgcaaat ttattaatgg cgaagaaata ttagaagaat ttttttgcgt
      661 taattgctga gattctaatt caaaatctaa tgaattagaa ttaaaaacag atgcggggtt
      721 ttgattaaat tcaataccat tagccattgg aaactcacgt aatcattaat tatgaatatt
      781 ccattaaata gtactgtaca acagcttcct ttctactcta ttcttagacg atgtaagttc
      841 accatatttt ttctcattgt tcaaccacca ggatgaataa aatttaaaaa tgttatcctc
      901 atataaaata aaacttctca atggtgcgat gcgtaatcgg gaattacaac ttccgatggg
      961 aaacctaacg ctaggtacgg aagataacga tattgtttac tttccccttg aacaaggcct
     1021 caatcagttt ttacttgata tacgtgagga aggtgttttc cttttatctc cggttgaatt
     1081 ttggattgat gggcaaccca cgccctatga agcagataaa ccattacctg tcggaaaggt
     1141 tattgatata gcgggatgtt gcttcatcat tggtgatatt gaccattcct tgccattgtc
     1201 tgatgtgcca gaaaggttct ctgccaaaag tcgacgcaaa aaacgcctta tcctggcgag
     1261 cgtgattggt gctacttttg cgttgtctgg tgccatcggc agttacgttt tgctttcccc
     1321 aaaagctgaa cccccaacgt ttaccagagc tgatgtatat cagcagctaa aagaaaacaa
     1381 acttcatgca atcactctgg tatggcatgg gaaaaatatc gcgctttatg ggcgttgcga
     1441 atcaaccacg gatttaactc cattttttaa ttacctaaaa gaaaaaaata ttttttatta
     1501 caataatatc atttgcaata accaaatcat atccgcaatt aatgatgtct taacggaata
     1561 cggttacaaa gatatcatta ttacaaaagg taataagcct ggattctttc tgttatcggg
     1621 atacattccc ccttcaccca aatggagcga ggttgaaaat ctgttactga atacgccagg
     1681 agttgcagga tgggaaatac acaacaacag taacaataaa atcaatgaac tggcatcaga
     1741 gtttaaaaag aataaactaa taaactatgt caatattttc aaaaagaatg atgtcatcat
     1801 tgttgcgggg gaagtttctc aacagaatga gagtaagatc cttgccatta taaatgcaat
     1861 gaacaaaaat agtaacgcca agatactctt tcagaacatt cagccgtaca tttcagcaga
     1921 tattttcccc ggaaaaatac tcagaatcag cggtacgatg aagaatccaa caattgctct
     1981 tgataatggc accagcctgg ggattggtag cattctgaaa ggaggctatg tcatagacgc
     2041 gattgatcct aaagatggta tcaacatctc ccgcccagac gaatatatac atattccact
     2101 gtcgtattaa tttcttatcc tcttccccct cttcggaggg ttttttattg tgtggatgtt
     2161 gtgagcatag ttgttgcttg atgtgattca gctttggtat cagcgtggtt ggatcaacct
     2221 acatgagaaa acgtgaatgt gtctctttga aagagaccta ttaagcagat aaaaagacca
     2281 gtagtagatt tgattataag aggttatttc ttaagaagaa ataattatgc cccgactaaa
     2341 acaatacata ttttagccgg ggtggttatg gaattattct acacaaaccg catagacatt
     2401 tggagtatta acattaaccc caggaagagg gttttgtgtt attaggttat aagtgcttga
     2461 tactccagaa cgctgctcac tagatgtctg tttaatccaa gcagttattg agttcataga
     2521 actataatgg ctatatttat ttgcagcccc ccatgagtca taaatatctg acaataccgt
     2581 ctgtgtggat ggtaataaat ttttgcaaat ggacatagca tcagcataat aggcttgctt
     2641 atccactttt atcatatacg acggtgcttt tatagtgtaa cttactgttt gcttatcacc
     2701 agatgtggct ttaattacga cactgccttt accattcaaa gtgactttcc ctgatgcatc
     2761 gacagtcgcg atactggtat tttctgaata ccatgaatat gtaccatcac caccgcttgc
     2821 tttcagttta aactgaccat attgcagcca aatattaggc aactcgcctc tgacattgtt
     2881 accaataata tcaaccttgt tgtcaatttt cagttcatca aaaaaagtga cctcagtcgc
     2941 tttaacctca gccccatcac tgactgtcgc actaacagtc gctttaccgg cggaactgga
     3001 agttagtgtt atcgtcgcac gaccatcatt tcccgtggtt gcttgcgttt gagacttacc
     3061 gttgaacatc ccaaagtttg ttgagaatgt aacggattga ttattaactg gctgaccgtt
     3121 tttcataact tttacagtat atttaatagc atccttacca tttgctactg cagttgtctt
     3181 atcagcctta atctcagtaa tgctggcctt ggtttgatca aaaaatataa ccgcactggc
     3241 attaagtgct gaagtcatct ccgcggtttt agcagacacg acgacctgtc ctggcgtact
     3301 cgacttcaac gttacggttg ccttaccgtt agcatccgtt ttggcactat ttgccccaag
     3361 agttgcagtt cctgaaacaa tattaaatga aacagggaca ttagcctgag ctaccccatt
     3421 ctttttcacc gtcgcggtat aagtaatggt atcggcgtta tccgctttag ccgaagtctt
     3481 atccgccgta aagtccgtta ccccaacctg gtcgacaact tgaccattcg acagaacggt
     3541 aatagtaagc tgtacattgt tagagctatt gccattacgg tcataggcgc gagccgtcac
     3601 tttataaata ttgctgccac cttgcacata agcaggcaaa atagcctggt agtcttgtgc
     3661 gctttggctt ccgctatgct gaatctgacc gccctgactg cgtaatgcac tatcatccca
     3721 gacgatacga tccagaccgt atttgctctt aacgatcaac tgaatcttct gcgtactgtg
     3781 ttcagtacca ttaatatcat gcggaatatt cagagaaaga atatcctgct tcttgtactc
     3841 cagaataata ttgttattac gctgaaccag atcgtaacgg ctgcctgata atgttcttaa
     3901 ctcgttaaca tactgtggtt caatttgctg agaccacgat ttatcaaact gataacggaa
     3961 ctgcattgag taaaggagat cattttcatt acccgtacca tgacggtaat cgatccccat
     4021 cgtcaccaga ggaatcggag tatagtttac accaacggtc gccgcaccag gattcgactg
     4081 cagcttatca gaattaaaca aagcaacatt atcaccataa tactgctcat atatcagctt
     4141 ggcgcctaat gccggatatg acggtagata gccattaaaa cggatatcga agccatttgc
     4201 tgggcgctca tcatagtctt tcttattgta tgactcatgc cagccgctca tgcggaaata
     4261 gccgttaacg ctacttttga aatagtctcg ccagtattcg ccaccaatac ctaaacgggt
     4321 attatcacca gaaaaatcct gatcaatgaa gacgttatag cccaacatgt ttgcaggaag
     4381 gaaaaaacgc tgacccgcac ctaaatttgc cgtaaagcgg gagtcaatgt aacgcgctcc
     4441 gacctgacca aatgccagca ttttttcgga atcatagaac ggtaataaga agtccagtga
     4501 actaccgtca aagttattac cactctgcag attaacctct gccgttccat aatgttgtaa
     4561 ccaggcctgc aactgtgacg aagcctggtt accagcgata ccaagagcgg tatctttcgc
     4621 gtaatcgccg ttcagagatc gcgactgaag ctggctaccg agactcgccg cctgttgtgc
     4681 cgcataattt aatgccttgt catcggtcat gttgcttttg gtcacgtccg gggacatttt
     4741 agtcagttta ttcgtgtgac cagcaacacc acctgcagca acaagaggtg ccgaacctaa
     4801 aagtggtagt gcactgtatt caaagggaag ttttttgagt ggcaaaatga tctgctgacc
     4861 aggcgcggcc ttcatcattt cgctttcaga actgtataaa tgcttattca acgaccaaat
     4921 cgtcgataaa
//
LOCUS       DAZKIF010000228         4821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZKIF010000228 DAZKIF010000000
VERSION     DAZKIF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.230"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            140..1471
                     /locus_tag="ACHOTW_004608"
     CDS             140..1471
                     /locus_tag="ACHOTW_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9846567.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            1499..2809
                     /gene="cyuA"
                     /locus_tag="ACHOTW_004609"
     CDS             1499..2809
                     /gene="cyuA"
                     /locus_tag="ACHOTW_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP9846568.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2943..3107)
                     /gene="yhaL"
                     /locus_tag="ACHOTW_004610"
     CDS             complement(2943..3107)
                     /gene="yhaL"
                     /locus_tag="ACHOTW_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP9846569.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(3130..3831)
                     /locus_tag="ACHOTW_004611"
     CDS             complement(3130..3831)
                     /locus_tag="ACHOTW_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP9846570.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            3936..>4821
                     /gene="yhaJ"
                     /locus_tag="ACHOTW_004612"
     CDS             3936..>4821
                     /gene="yhaJ"
                     /locus_tag="ACHOTW_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP9846571.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLF"
BASE COUNT         1047 a         1150 c         1399 g         1225 t
ORIGIN      
        1 tgattgtgaa gattttctct gacctcgcgc gtatcatttc tttattctaa taactcgcct
       61 gcaaaaacgt agaccgcata tcgccttgat gcggctcgga aaaaaataat aagccctaca
      121 aaatattgag cgagaaatta tggaaattgc atcgaataaa ggcgtcattg cagacgcttc
      181 gaccccggcg ggtcgtgctg gaatgagtga gagcgagtgg cgagaagcga tcaaattcga
      241 cagtactgac accggctggg tgattatgag tatcgggatg gcgattggcg cggggattgt
      301 ttttctcccg gtgcaggtcg gtttgatggg attgtgggta tttttgctct catcggtgat
      361 tggttacccg gcaatgtatc tgtttcagcg gttgtttatt aatacgctgg cagaatcacc
      421 agaatgtaaa gattacccga gcgtcattag cggttattta ggtaaaaact ggggcatcct
      481 gttaggtgca ctctatttcg taatgctggt gatttggatg ttcgtctatt ccaccgccat
      541 caccaacgat agtgcttcct acctgcatac cttcggcgtg acggaagggt tgctgtcaga
      601 cagtcccttt tatggtctgg tactgatttg cattctggtg gcgatctctt cacgcggcga
      661 gaaattgtta ttcaaaattt cgactggcat ggtgctgacc aagctgctgg tggtcgcggc
      721 gctgggcgtg tcgatggtgg gaatgtggca tctgtataac gtcggttcgc taccgccgct
      781 ggggctgctg gtgaaaaacg ctattattac gctgccgttt accctgacgt cgattctgtt
      841 tatccagacg ttaagcccga tggtgatctc ttatcgctcg cgggaaaaat cgattgaagt
      901 agcgcggcat aaagcattgc gggcaatgaa tatcgcgttt ggcattttgt ttatcatcgt
      961 ctttttctac gccgtgtcgt tcacgctggc gatgggacat gacgaagcgg taaaagccta
     1021 cgagcagaat atttccgcgc tggcgattgc cgcacagttt attagcggtg acggcgcagc
     1081 gtgggtgaaa gtggtcagcg tcattctcaa tatctttgct gtcatgactg cattctttgg
     1141 cgtctattta ggttttcgcg aagcaacgca agggatcgta atgaacatcc tgcgtcgcaa
     1201 gatgcctgcc gagaagatta acgaaaatct tgttcagcgc ggcatcatga ttttcgccat
     1261 tttgctggcc tggagcgcca tcgtactgaa cgcaccggtg ttgagcttca cctctatctg
     1321 tagcccgatt ttcggcttgg tagggtgcct gatcccggcg tggctggttt acaaagtacc
     1381 ggcattgcac aaatacaaag ggatgtctct gtacctgatt atcgtcactg gtttgttgct
     1441 ttgtgtttct ccgttcctgg cattttcttg atttacctga aattttaagg tttttaatat
     1501 gtttgattcg actttaaatc cgttatggca gcgttacatc ctcgccgttc aggaggaagt
     1561 aaaaccggcg ctgggatgta ctgaaccgat ttcactggcg ctggcggcgg cggttgctgc
     1621 ggcagaactg gaaggtccgg ttgaacgtgt agaagcctgg gtttcgccaa atctgatgaa
     1681 gaacggtctg ggcgtcaccg ttcccggcac gggaatggtg gggctgccga ttgcggcggc
     1741 gctgggggcg ttaggtggaa atgccaacgc cgggctggaa gtgctgaaag acgcaactgc
     1801 gcaggcaatt gccgatgcca aagcactgct ggcggcgggg aaagtctccg ttaagatcca
     1861 ggaaccttgc aatgaaatcc tcttctcacg cgccaaagtc tggaacggtg agaagtgggc
     1921 gtgtgtcact atcgtcggcg ggcataccaa cattgtgcat attgagacgc acgatggtgt
     1981 ggtgtttacc cagcaggcgt gtgtggcaga gggcgagcaa gagtctccgc tatcggtgct
     2041 ttccagaacg acgctggctg agatcctgaa gttcgtcaat gaagtcccgt ttgcggcgat
     2101 ccgctttatt ctcgattccg cgaagctaaa ttgtgcgtta tcgcaggaag gtttgagcgg
     2161 taagtggggg ctgcatattg gcgcgacgct ggaaaaacag tgcgagcgcg gtttgctggc
     2221 gaaagatctc tcttcatcca ttgtgattcg taccagcgcg gcatccgatg cgcgtatggg
     2281 cggcgccacg cttccggcaa tgagtaactc cggctcgggt aaccagggga tcaccgcaac
     2341 aatgcccgtg gtggtggtag cagaacactt cggagcggat gatgaacggc tggcgcgtgc
     2401 gctgatgctt tctcatttga gcgcaattta catccataac cagttaccgc gtttgtctgc
     2461 gctgtgtgcc gcaacgaccg cagcaatggg ggccgccgcc gggatggcat ggctggtgga
     2521 tgggcgttat gaaaccatct cgatggcgat cagcagtatg atcggcgatg tcagcggcat
     2581 gatttgcgat ggtgcgtcga acagctgcgc gatgaaggtt tcgaccagtg cttcggctgc
     2641 gtggaaagcg gtgttaatgg cgctggatga taccgccgtg accggcaatg aagggatcgt
     2701 ggcgcatgat gttgagcagt cgattgccaa cctgtgtgcg ttagcaagcc attcgatgca
     2761 gcaaacggat cggcagatta tcgagattat ggcgagcaag gccagataaa cgccaggttt
     2821 tgggtatcgt gccggatgcg atgctggcgc atcttatccg gcctacgggt tgtttaattc
     2881 gttaatgttg ttggattttg taggccggat aaggcgtttt cgccgcatcc ggcaacctgt
     2941 ctttacgcgg tagcttcatc ctcggctaaa cgcgtttcag catccgcgac gatggcttcc
     3001 agctcgctca acatctcttc atagccaaaa tttttggcgg tgcgagcagg ttctttcgcc
     3061 accacgggct tcactggctg gcgctttttg gccgattttt tactcatcat catctccctg
     3121 taaaaacaac tagacaggca aatctatcag caaagcgcgc agtggggaat cggcaaccag
     3181 cgtaatgtta gcctcgtcac gaataaacgc cccatcaccg caggtcagcg ccgctttctc
     3241 ttcatgatgc gtaagcgcat gaaatttccc gtgaatcgat tgcaaatacg tgcgtggccc
     3301 atgcaactgg aaattcgcac tttcgccttt gtcgagcacg atatggtgca gccacacttg
     3361 ctggcgtaat tgcaggcttc ccatcgcacc ctctggcgag gcgattaaat gctgcttgcc
     3421 catattaagc gccagttttt gaatcagcgg attctctcgc tgcgggcagg cgtccagcca
     3481 aagctgcatt cgtgttaacg gtttgtcttt gctgagattg tgttcgctgt agctgacacc
     3541 cggctgggta gagagcagca acgcctcacc ggcgctggcc tgaacatgat tgccttcgct
     3601 gtcgcgatac tctgcttctc catccagaat cacatttaaa atatcgactt tgggataggt
     3661 tcgcggctga aaggcggcac ctggggccag cacttcctgg ttaagcacac gcagggaggc
     3721 atagcctaac aatttcgggt cgaagtagtg tccaaaggaa aaagtatacc gggcctgcaa
     3781 ccatccgtag tctgcttgtc cacactgcct ggcagttcgg gtagtaatca taattcttga
     3841 cctctcttta cttcctttta tggtaagggg ctggacgcta cattgttagc cagatattct
     3901 gcccggtatg ttcaaatttc ctgaatgaga acgaaatggc caaagaaagg gcattaacgc
     3961 tggaagcact acgggttatg gatgcgatcg atcgccgggg cagttttgcg gcggcagcgg
     4021 atgagctggg acgcgtgcct tccgcactta gctacaccat gcaaaaactg gaagaagagc
     4081 tggatgtggt gctgtttgac cgctcgggcc atcgcaccaa attcaccaat gtcgggagga
     4141 tgttgttgga gcgagggcgc gttttgctgg aagccgcaga taaactgact actgatgcgg
     4201 aagctctcgc gcgcggttgg gaaacgcatc tcaccattgt gaccgaagcg ctggtaccga
     4261 cacctgcctt tttcccgtta atcgacaaac tggcggcaaa agccaatacc caactggcaa
     4321 tcatcacaga agtgctggcg ggggcgtggg aacggctgga gcaggggcgg gcggatattg
     4381 ttatcgcgcc ggatatgcat tttcgttcct cgtcggagat caactcgcgc aagctctata
     4441 cgttaatgaa cgtctacgtt gccgcgcctg atcacccgat tcatcaggag ccggaaccgt
     4501 tatctgaagt gacgcgtgtg aaatatcgtg gaattgcggt ggcggatacc gctcgtgagc
     4561 gcccggtgtt gactgtacag ctgctggaca aacagccgcg cttaacggtg agcacgattg
     4621 aagataaacg tcaggcatta ctggcggggc ttggcgtggc gacgatgccg tatccgatgg
     4681 tcgaaaaaga tattgcggaa gggcggttgc gtgtcgtcag cccggaatcg accagcgaga
     4741 tcgatattat tatggcctgg cgtcgtgaca gtatggggga agcgaaatcc tggtgtctgc
     4801 gggaaattcc caaacttttt a
//
LOCUS       DAZKIF010000229         4782 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZKIF010000229 DAZKIF010000000
VERSION     DAZKIF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.231"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            161..727
                     /gene="slp"
                     /locus_tag="ACHOTW_004613"
     CDS             161..727
                     /gene="slp"
                     /locus_tag="ACHOTW_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP9846572.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            883..1413
                     /locus_tag="ACHOTW_004614"
     CDS             883..1413
                     /locus_tag="ACHOTW_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9846573.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1474..2502)
                     /gene="chuS"
                     /locus_tag="ACHOTW_004615"
     CDS             complement(1474..2502)
                     /gene="chuS"
                     /locus_tag="ACHOTW_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFP9846574.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(2551..4533)
                     /gene="chuA"
                     /locus_tag="ACHOTW_004616"
                     /gene_synonym="shuA"
     CDS             complement(2551..4533)
                     /gene="chuA"
                     /locus_tag="ACHOTW_004616"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFP9846575.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVGASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1277 a         1170 c         1080 g         1255 t
ORIGIN      
        1 tactcatgcc acgccccaaa ataacggaat tgaactaaac agaagggata taaacatcag
       61 acaggtttac gttactatca ggcatatcgc ctcagaatca gatgaaaact ataaagaaat
      121 atctattatg gttttaatat ttgttgataa ggatagtaac atgaacatga caaaaggtgc
      181 actcatactc agcctttcat ttttgcttgc agcatgtagt tcaattccgc aaaatatcaa
      241 aggaaataac caacctgata ttcaaaaaag ttttgttgct gttcataacc agccggggtt
      301 atatgttggt caacaagcgc gctttggtgg gaaggttatc aacgttatca atggcaaaac
      361 ggatacgttg ttagaaatcg ctgtattacc gttggatagc tatgcgaagc ctgatattga
      421 agccaactat cagggccgac tgctcgccag acaaagcggc ttccttgatc cagtgaacta
      481 tcgtaatcac tttgttacca tcctcggcac cattcagggt gaacaacctg gctttatcaa
      541 taaagtcccg tataacttcc tggaagtgaa tatgcagggc atccaggtgt ggcatttgag
      601 agaagtggtt aataccacct ataacctgtg ggattacggc tatggtgcat tctggccgga
      661 accgggctgg ggtgcgcctt actacaccaa tgcggtgagt caggtaacac ctgagctggt
      721 caaataacac caccgaaaga tgcagatgct ttctcagcat ctgcatcatg cattacatca
      781 aattaataca cagtaagcta actattatta ttataagccc tgtcctgtta attacctttg
      841 gcaaactgat tataaagtta atgtccgcac caggagtcgg ttatgtttct tataattacc
      901 agggatacga tgttcttcac cgcgatgaaa aacattctga gtaaaggtaa tgtcgttcat
      961 atacagaacg aagaagagat cgacgtaatg ttgcatcaga atgccttcgt cattattgat
     1021 acattaatga ataatgtatt tcattctaat tttctcactc aaattgaacg attaaaacct
     1081 gtccatgtca ttattttctc cccctttaat attaaacgct gcctggggaa agtgccggtg
     1141 acctttgttc cgcggactat cactatcatt gattttgtcg cactcatcaa tggcagttac
     1201 tgctctgtgc ctgaagcgaa tgtgtcactt tcgcgcaagc aacatcaggt tctgagctgc
     1261 attgcgaatc aaatgacaac ggaagatatt ctggagaaac tgaaaatatc gctaaaaacg
     1321 ttctactgcc ataaacacaa tatcatgatg atcctcaatc ttaagcggat caatgagctg
     1381 gtacgccatc agcatattga ttatctggtg tgaacgattg atcaatataa agaggcccag
     1441 caataaatag ccagacctcc cgttaattat acgttatgca gcaacgcctt ccggtatcag
     1501 cgaagcaatt tgcttacgcc attgcgcttg ctcctgttcg ccttctgtac gttgaccata
     1561 aagttgcgct atctgcgtac catcatgggc aaacaattcc agactggtta cgtagccatc
     1621 gctggtcggt ttacgggtaa cccaggcttc agcaatgctc tcttctaata gatgaagagt
     1681 aaacgtcggg ttgaaaatat tcagccaacc tttcattggc accacttttt ctaccacacc
     1741 ggtgaaaatc tgtacgcagc cacggttgcc aacaaacacc atgatttcat taccatcctg
     1801 ctgtgcagat tcaagaattt gcgccaacgc actgttggat actttgcagg ccaaatcgtc
     1861 tgccaccaga ttgaacgcct gttggcgcgt caggttgtgg cgcttgagca acgtaaaaaa
     1921 ctgatgaacg tcggtcatcg cccgccactc ttgctcgacc acagtggcat cggctcgcgt
     1981 ttgaacaact ggcgcatcaa cggcttttaa ctcaagcggc gtattctcat cggtgataaa
     2041 ccgtgccaga agctcactcc atgccgccat atcggtattg tcggtggcat aaacttttag
     2101 taatgcatcg ccctgatgat caaagaactg aatactctgg cgttcgccac gagccgtgtt
     2161 ttctttgatg tggaaaacac tggcccattg attgagaaac agacgtaaat ccagcgcgcg
     2221 cggattgagg atcaatccgg catgtccgtt caaatgctgg tttgtgaacg taccaacttg
     2281 ctcatggact gcatattcat tacgacaaat acatttggtt tcgccaacac tttcgagcgc
     2341 cgccagaatt tcacggatat cgccgtgcat ccgccacgca tcgtgcgtga cgcgtgcaaa
     2401 tgccagttct gcttctctaa tattcattaa ccctgcgatg tcacgcgcgt actttccggg
     2461 attttgttct tttaactcaa gccagcgtgt gtagtggttc attgtctctc ttccttccag
     2521 ataaatgccc cgaaatatcg gggcagatga ttaccattga taactcacga aaatttttcc
     2581 gttacgacca tcctgtggga tgccttgcgg cgaccagtac tctttgtcga aagcgttacc
     2641 caacaccaaa gtagtggtca tacctttgag cgcctgttgt ccttgataac tgacgtagaa
     2701 atcattcacg ccatagcctg gttgtttgct gtaactgctg ctgatatgtg ttgagcgatc
     2761 ggcaaacgta ccaacccacc caacagagaa gccactgtga gcgatcggaa tattcagagt
     2821 gctggtaaca gtatccgggt taatgctgga gatgtattcg ccggtatcgg tgtctttgcc
     2881 gcgggtacgg ttataggcca catcaaggct aaacagatca gtggtatatt tcgtcatcac
     2941 atcccagccc cagattttgg cgttcgggac gttatacgac atagtcgtcg ccgccgcgaa
     3001 atcgacggtc gtggagatgt aatccttcgc tttggtatca aagtagctgg ctttaaattc
     3061 cagagcatca ttggacaaca tcaggtcatc aaaacgcagc ccaaaaccgt actcctgagt
     3121 ttcgttagtt tccggacgta agttcgggtt tggcacccaa tagttggtat agaagcgacc
     3181 aatcgagaag tgcttagaat cgttatacat ttcgcccatc gtcggggcgc ggaatgcctg
     3241 ggcatatgag ccaaataaca tcagccagtt agtcggattg atagtcatcc ccgcacgaga
     3301 tgaccatttg tcggcatcaa catctttgta accgtcactg ctaccgcgat aactgtcata
     3361 gcgggttccg ccaagcaggg taatcggcag atcgcgtaag gtgatctcat cctgtagcca
     3421 gccggagcta aaatcgattt ttgcttgcgg gaagcccgtc gtcgcgccgc ccggatgttg
     3481 ttcctgacga taatactcac cgccatatgt cagtaagtga gaagcgaaac tgtcggcaaa
     3541 gagagtggaa cggttctcca gcctggctcc ttttgttatc tgttcacgat actcgccgga
     3601 actccccgtg ttttgcgcat taatacggac ttccgaccaa taaatttttg catctgcatt
     3661 taaccagtcg ttgccctgcg gggcgagttt ataagaaagc tgcgcatcgc gttgaattgt
     3721 tgaacgatca accatcgggt tgctgctttc agaagcccca acggtctgcg gattttttgg
     3781 ttcacgcgcg tcgttgttgt agtaacgcac taaaccgctc agagactggg ctgaatcaat
     3841 ttgccaggtc cctttcgcca gcatgttatt aatggactcg tcattcggcg cggtttcacc
     3901 attgctctgg cgtaaatcac cccgatcgcg actggaccag gccacaatac catccagatt
     3961 ttcagttcgc ccaaacgcgc tcgcgcctaa tcccaggcta tggtcccccg tgccgccagt
     4021 accaaagaca cgaaaaccac tgctttgtcc ttcctgcaat aaatcttttg catcgaccgt
     4081 atcgtaggag atcactccac ccagcgcgcc actgccatac agtaatgctg aaggtccacg
     4141 aacaatctca acacgcttga tcagcgccgg atcgagaaaa gtgccattca ggtgtccggt
     4201 atccgttccc tgacgaacac catcgacaag aaccagcacg ccgcgatgat catagccacg
     4261 catatttaca tcctgaccgt tggttcgtcc ggtaccatcc agagtaattc caggaacatg
     4321 acgcagcaga tcggtggctg aagtagccgt ttgattttca ggagcggaag tgtcgatgac
     4381 gctgaccatc ataggcgctt cgaaggaact acgggcattc cccgttgccg taacggtcat
     4441 ggtttcagta gcaaaagcaa acgttggcaa ggtggcagaa acagctaagg ccaataaact
     4501 caaacgcaac gaggtaaatt gcggacgtga catagcgatt ctccatgagg aaaataaaaa
     4561 atgctggctg cctgggttaa taccggggtg gctggcgaaa aataaattta tttaaaattc
     4621 agagcgataa tattcgccgg gtgagtttac aagcgtttat atcccatgaa attgcctgat
     4681 aactcggcaa ttacgcaaaa gcacgccctc tttgtcaggc aaaacgatat gccccatcag
     4741 gagtgaagag agctgtgcgt tatcccttgc aaatgctatg gt
//
LOCUS       DAZKIF010000230         4722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZKIF010000230 DAZKIF010000000
VERSION     DAZKIF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.232"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1247
                     /gene="garP"
                     /locus_tag="ACHOTW_004617"
     CDS             <1..1247
                     /gene="garP"
                     /locus_tag="ACHOTW_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP9846576.1"
                     /translation="YADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYLLMQIPGG
                     WLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSEAPSFPA
                     NARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTVMGVIGFV
                     LTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHYIKQLLS
                     NRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAGGVLG
                     GVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFGKGFG
                     ALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVFVGCS
                     ALMAMVCYLFVVGDIKRMELQK"
     gene            1263..2033
                     /gene="garL"
                     /locus_tag="ACHOTW_004618"
     CDS             1263..2033
                     /gene="garL"
                     /locus_tag="ACHOTW_004618"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP9846577.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2063..2953
                     /gene="garR"
                     /locus_tag="ACHOTW_004619"
     CDS             2063..2953
                     /gene="garR"
                     /locus_tag="ACHOTW_004619"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9846578.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3050..4195
                     /gene="garK"
                     /locus_tag="ACHOTW_004620"
     CDS             3050..4195
                     /gene="garK"
                     /locus_tag="ACHOTW_004620"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP9846579.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4228..4604
                     /gene="rnpB"
                     /locus_tag="ACHOTW_004621"
     ncRNA           4228..4604
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHOTW_004621"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1085 a         1145 c         1375 g         1117 t
ORIGIN      
        1 actacgccga tcgtgcaacg ctgtctattg ctggtaccga agtggcaaaa gagttgcagt
       61 taagtgcggt ttcgatgggt tacatctttt ctgcttttgg ctgggcctac ttgctgatgc
      121 aaatccccgg cggctggctg cttgataagt ttggctcgaa aaaagtttac acctacagcc
      181 tctttttctg gtcgctattc accttcctgc aaggctttgt tgatatgttc ccgctggcct
      241 gggcagggat ctccatgttc tttatgcgct ttatgctcgg cttctcggaa gcgccatcat
      301 tcccggcgaa cgcccgtatt gtcgccgcct ggttcccgac gaaagaacgt ggtactgcct
      361 ccgccatctt taactcggcg caatatttct cgctggcgct cttttcgcct ctgcttggct
      421 ggctgacttt cgcctggggc tgggagcacg tctttaccgt tatgggggtg attggttttg
      481 tgctgacggc gctgtggatc aaattgattc ataacccgac agatcaccca cgtatgtctg
      541 cggaagagct gaagtttatc tctgaaaatg gcgcggtggt cgatatggac cacaaaaagc
      601 cgggcagtgc ggcagcaagc ggacccaaac tgcattacat caagcaattg ctctctaacc
      661 gcatgatgtt gggcgtattt ttcggacaat attttatcaa caccatcacc tggttcttcc
      721 tcacctggtt cccgatttat ctggtgcagg aaaaaggtat gtcgattctg aaagtgggtc
      781 tggtcgcctc gattccagca ctgtgtggtt ttgcgggcgg cgtgctggga ggtgtcttct
      841 cggattatct gatcaaacgc ggtttatccc tgaccctggc acgtaagcta ccgattgtgc
      901 tgggaatgtt gctggcttcc accatcatct tatgtaacta caccaacaac accacgctgg
      961 tggtcatgct gatggcgctg gctttctttg gtaaaggatt tggtgcgctg ggctggccgg
     1021 tgatttctga caccgcgcct aaagagattg ttggcctctg cggcggcgtc tttaacgtct
     1081 ttggcaatgt tgcctccatt gtcactccac tggtgattgg ctacctggta agtgaactgc
     1141 actccttcaa tgcagcactg gttttcgtgg gatgttcagc gctaatggcg atggtctgct
     1201 acctcttcgt agttggcgac attaaacgta tggaattgca gaaataagca aaggtataag
     1261 cgatgaataa cgatgttttc ccgaataaat tcaaagccgc actggctgcg aaacaggtac
     1321 aaattggttg ctggtcagca ctctctaacc cgattagcac tgaagttctt ggtttggctg
     1381 ggtttgactg gctggtgctg gatggcgaac atgcgccaaa cgatatttca acgtttattc
     1441 cgcagttaat ggccctgaaa ggcagcgcca gcgcgccagt agtgcgagtg ccgaccaacg
     1501 agccggtaat tattaagcgt cttctggata tcggtttcta taacttcctg attccttttg
     1561 tagaaacaaa agaggaagca gagcaggcgg tggcatcgac gcgttatccg ccagaaggca
     1621 ttcgcggcgt ctccgtttct caccgcgcca atatgtttgg caccgtggcg gattatttcg
     1681 ctcagtcgaa caagaacatc actattctgg tccagataga aagtcagcag ggcgtagata
     1741 acgtcgatgc tattgccgct accgaaggcg tagacggcat cttcgtcggc cccagcgatc
     1801 tggccgcggc attaggccat ctcggcaatg catcacaccc ggatgtacaa aaagcaattc
     1861 agcacatttt taaccgtgcc agcgcgcacg gcaaacccag cggtatcctc gcgccggtcg
     1921 aagccgatgc gcgtcgttat ctggaatggg gcgcgacgtt tgtggctgtc ggcagcgatc
     1981 tcggcgtctt ccgctctgcc actcagaaac tggctgatac ctttaaaaaa taaccaccac
     2041 cgcaacaaga gagatgattg atatgactat gaaagttggt tttattggcc tggggattat
     2101 gggtaaacca atgagtaaaa accttctgaa agcaggttac tcgctggtgg ttgctgaccg
     2161 taacccagaa gctattgctg acgtgattgc tgcaggtgca gaaacagctt ctacggctaa
     2221 agcgatcgct gaacagtgcg acgtcatcat aaccatgctg ccaaactccc ctcatgtgaa
     2281 agaggtggcg ctgggtgaga atggcattat tgaaggcgcg aagccaggta cggtattgat
     2341 cgatatgagt tctatcgcac cgctggcaag ccgtgaaatc agcgaagcgc tgaaagcgaa
     2401 aggcattgat atgctggatg ctccggtgag cggcggtgaa ccgaaagcca tcgacggtac
     2461 gctgtcagtg atggtaggcg gcgacaaggc tattttcgac aaatactatg atttgatgaa
     2521 agcgatggcg ggttccgtgg tgcataccgg ggaaatcggt gcaggtaacg tcaccaaact
     2581 ggcaaatcag gtcattgtgg cgctgaatat tgccgcgatg tcagaagcgt taacgctggc
     2641 aactaaagcg ggcgttaacc cggacctggt ttatcaggca attcgcggtg gactggcggg
     2701 cagtaccgtg ctggatgcca aagcgccgat ggtgatggac cgcaacttca agccgggctt
     2761 ccgtattgat ctgcatatta aggatctggc gaatgcgctg gatacttctc acggcgtcgg
     2821 cgcacaactg ccgctcacag ctgcggttat ggagatgatg caggcactgc gtgcagatgg
     2881 tttaggaacg gcggatcata gcgccctggc gtgctactac gaaaaactgg cgaaagtcga
     2941 agttactcgt taatgacgtc gtgcccggat ggcgtattgc aatccgggcc tggaatccag
     3001 gccgaataag agaaacgccc tccggcgtca tgtggtaaca ggcataggta tgaaaatcgt
     3061 aatcgcccca gactcttata aagaaagttt atctgccagc gaggttgcgc aggcgataga
     3121 aaaaggattt cgggaaattt ttcctgatgc acagtacgtt tctgttcctg ttgccgacgg
     3181 tggcgaagga acggtggaag cgatgattgc agccacccag ggttccgaac gtcacgcctg
     3241 ggttacaggg ccgctgggcg agaaagtgaa tgccagttgg gggatctccg gcgatggcaa
     3301 aaccgcgttt attgaaatgg cggcggccag tgggctggag ctggtacctg cggaaaaacg
     3361 cgatccactc gtgaccactt cacgcggcac aggcgagtta atcctgcagg cgctggagag
     3421 cggcgcgaca aacattatta tcggcattgg cggcagcgct acaaatgatg gcggcgcagg
     3481 catggtacag gcgctggggg cgaaattatg cgacgccaac ggcaatgaaa ttggttttgg
     3541 cggcggtagt cttaatactc tgaatgatat tgatatttcc ggcctcgatc cgcgcttaaa
     3601 agattgcgtc attcgcgtcg cttgtgatgt caccaatccg ctggtgggcg ataacggcgc
     3661 atcgcgcatc tttggcccac aaaagggagc cagtgaagcg atgattgttg agctggacaa
     3721 taacctctct cactatgccg aggtcattaa aaaagcgctg catgttgatg tgaaagatgt
     3781 ccccggcgca ggagctgcgg gtggtatggg cgcggcgcta atggcgtttc ttggtgcgga
     3841 actgaaaagt ggtattgaaa tcgtcactac ggcgctgaat ctggaggaac atattcacga
     3901 ttgtacgctg gtgatcaccg gtgaagggcg tattgacagc cagagtattc acggcaaggt
     3961 accgattggt gtcgcaaacg tggcgaagaa gtaccataaa ccggtgattg gcattgcggg
     4021 tagcctgacc gatgatgttg gcgttgtaca tcagcatggc attgatgcgg tcttcagcgt
     4081 attgaccagc ataggtacgt tggacgaagc attccgcggg gcttatgaca atatctgccg
     4141 tgcttcacgt aatatcgccg cgacactggc gattggaatg cgcaacgcgg ggtgacaagg
     4201 gcgcgcaaac cctctatact gcgcgccgaa gctgaccaga cagtcgccgc ttcgtcgtcg
     4261 tcctcttcgg gggagacggg cggaggggag gaaagtccgg gctccatagg gcagggtgcc
     4321 aggtaacgcc tgggggggaa acccacgacc agtgcaacag agagcaaacc gccgatggcc
     4381 cgcgcaagcg ggatcaggta agggtgaaag ggtgcggtaa gagcgcaccg cgcggctggt
     4441 aacagtccgt ggcacggtaa actccacccg gagcaaggcc aaataggggt tcataaggta
     4501 cggcccgtac tgaacccggg taggctgctt gagccagtga gcgattgctg gcctagatga
     4561 atgactgtcc acgacagaac ccggcttatc ggtcagtttc acctgattta cgtaaaaacc
     4621 cgcttcggcg ggtttttgct tttggagggg cagaaagatg aatgactgtc cacgacacta
     4681 tacccaaaag aaagcggctt atcggtcagt ttcacctgat tt
//
LOCUS       DAZKIF010000231         4688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZKIF010000231 DAZKIF010000000
VERSION     DAZKIF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.233"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..102)
                     /locus_tag="ACHOTW_004622"
     CDS             complement(<1..102)
                     /locus_tag="ACHOTW_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFP9846580.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLI"
     gene            complement(175..543)
                     /locus_tag="ACHOTW_004623"
     CDS             complement(175..543)
                     /locus_tag="ACHOTW_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFP9846581.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPSMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            804..1385
                     /locus_tag="ACHOTW_004624"
     CDS             804..1385
                     /locus_tag="ACHOTW_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HFP9846582.1"
                     /translation="MNNSWWQELMHFFLQGMTLKQLIHMLIILIILIIVMPVSVKEWI
                     NLHNPEILPHYWMYYILLFCVSYVLNGVVNSAYHAVTERIEIFAAQKRKSKEEKYVQD
                     LFDSLTLGERAYLAFAVAANNQLKTEKGSPEAISLLEKGLLIRVPSATGYPEIDRFVI
                     PERYRNECYIRFAGKKDSLMDELIAQDKHGKNK"
     gene            complement(1402..1674)
                     /locus_tag="ACHOTW_004625"
     CDS             complement(1402..1674)
                     /locus_tag="ACHOTW_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846583.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLRCKKADECSGSICLPNVAIYAAGYRKSKQLTAR"
     gene            complement(2187..2738)
                     /locus_tag="ACHOTW_004626"
     CDS             complement(2187..2738)
                     /locus_tag="ACHOTW_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846584.1"
                     /translation="MNAAQSSLDEGKLVIAYSDKNGSTVGLEFSSVASSQATLLMKAC
                     SVAASDKEKRIVTSVVMDDTEIIQTTSDDGGDDMDKRLAVLEAEVAHIKSSMAGIKED
                     TRKISSDSTDAKRDTAVLLQKSLDFDASLSKKPSVDYFEAKFSALETKIADVKVWMLG
                     VLLASLAMPTIFFLINLYLKKGQ"
     gene            complement(2745..3026)
                     /locus_tag="ACHOTW_004627"
     CDS             complement(2745..3026)
                     /locus_tag="ACHOTW_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846585.1"
                     /translation="MSDKQEVSLAHINDVLESKNYIETNADGVVTIGTDNNGYEVFNF
                     VFLNSTPVIGHMNGEIAVAGMQRTKVASVTLSKQKAFDFYQSLKSMFEE"
     gene            complement(3149..3796)
                     /locus_tag="ACHOTW_004628"
     CDS             complement(3149..3796)
                     /locus_tag="ACHOTW_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9846586.1"
                     /translation="MKTMHEIIGERIKSLREAKGLSQAQLAKLCGWAAPSRLGNYELG
                     TRKVSADDALVLGAALGVSPAKIMFGEDSDAVFRQYEYPLFSSVQAGPFSEVGSYTAS
                     DAKAWVPTTTKASEKAFWLEVKGHSMTAPQGVRPSFPEGMLILVDPAEPVESGDFCVA
                     SANGDSEATFKKYEKDAGVSYLVPLNPAYRTLDCDHSCRIIGKVVKAQWPEETFG"
     gene            3905..4123
                     /locus_tag="ACHOTW_004629"
     CDS             3905..4123
                     /locus_tag="ACHOTW_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9846587.1"
                     /translation="MNKIAQQRKKIGVSQAVLASAIGWGQSRIANYELNIRTPGLNDC
                     RMIVEGLRKLGCQCSLDDVFPPSSNKAA"
     gene            4238..4534
                     /locus_tag="ACHOTW_004630"
     CDS             4238..4534
                     /locus_tag="ACHOTW_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP9846588.1"
                     /translation="MAQASYSKPTQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGITKKKSPVAAGDSKQIDMQF"
     gene            4567..>4688
                     /locus_tag="ACHOTW_004631"
     CDS             4567..>4688
                     /locus_tag="ACHOTW_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9846589.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYS"
BASE COUNT         1268 a         1064 c         1023 g         1333 t
ORIGIN      
        1 gataaggcgt ttccatccgt cacgtaattt acgggtgatt cgttcaagta aagattcgga
       61 agggcagcca gcaacaggcc accctgcaat ggcatattgc atggtgtgct ccttatttat
      121 acataacgaa aaacgcctcg agtgaagcgt tattggtatg cggtaaagcc gcactcaggc
      181 ggccttgata gtcatatcat ctgaatcaaa tattcctgat gtatcgatat cggtaattct
      241 tattccttcg ctaccatcca ttgaaggcca tccttcctga ccatttccat cattccagtc
      301 gaactcacac acaacaccat atgcatttaa gtcgcttgaa attgctataa gcagagcatg
      361 ttgcgccagc atgattaata cagcatttaa tacagagccg tgtttattga gtcggtattc
      421 agagtctgac cagaaattat taatctggtg aagtttttcc tctgtcatta cgtcatggtc
      481 gatttcaatt tctattgatg ctttccagtc gtaatcaatg atgtattttt tgatgtttga
      541 catctgttca tatcctcaca gataaaaaat cgccctcaca ttggagggca aagaagattt
      601 ccaataatca gaacaagtcg gctcctgttt agttacgagc gacattgctc agtgtattca
      661 ctcgttggaa tgaatacaca gtgcagtgtt tattagtatg cctgtctttt aaccacatca
      721 ggctcggtgg ttctcgtgta cccctacagc gagaaatcgg ataaactcta ttcaccccta
      781 cagagagcaa aagagaaacg ccgatgaaca actcatggtg gcaggaacta atgcattttt
      841 tcctgcaagg aatgacactt aaacagttga ttcatatgct aatcatcctg attatattga
      901 ttattgttat gcctgtaagc gtaaaagaat ggataaacct gcataatcca gaaatccttc
      961 ctcattactg gatgtattac atcctgttgt tttgcgttag ctatgtgctt aacggcgttg
     1021 ttaattccgc ttatcacgct gtgactgaaa gaattgagat attcgctgct cagaagcgca
     1081 aatctaaaga agagaaatac gtgcaagatt tgtttgattc gttaactctt ggagaaagag
     1141 cgtatttggc attcgctgta gccgctaata accagctaaa gacagaaaag ggaagccctg
     1201 aagcaatctc attgctcgaa aaagggcttc ttattcgggt accttctgct actggatatc
     1261 ctgaaatcga ccgttttgtt atcccggaac gctatagaaa tgagtgctac attaggtttg
     1321 ctgggaagaa agacagtctt atggatgaac ttatcgctca ggataagcat ggcaaaaaca
     1381 agtaattagc aaattaattt atcatctcgc cgtcagttgt tttgatttcc ggtagcctgc
     1441 cgcgtaaatg gctacgtttg gcaggcaaat acttccactg cattcatctg ccttcttgca
     1501 gcgaaggctt ccgagtgatg ctgctttgtc tgctctgacg caaccagaga gctttagcgc
     1561 aatttttcgc gccagtgctt cattactgcg tcgctcggca ataagttctg ctctgcgagc
     1621 tttgtagcgg ctttttgccg tacctttgga ttctttccag acaatggtta ccatgatggt
     1681 ctcctttaag tggctttggc gcatgacgcg tcgaggtgtt tatcttctcg atcgctgtct
     1741 tgcagctgca attcgcgcca tccccaaaac cactcaagtt ctggtctcaa cggttaggtt
     1801 gagagttcgt cgatgttaaa gagcctgcca atctgttcct tgtggcgtct cagcgtcctg
     1861 ctgatggaaa tgatagtcac atattgtgat ttaatggtca atcacaaaat gtgtaaaata
     1921 aaggtgtgac acgttatgtg tatgattttt ttgtgtaaat agttttaccc gcgatggttt
     1981 tgcttactta agtcggtggc aacagcagga tgggtgctga aaagttcgaa aaacgagcga
     2041 atggggtggt ggcgagatgg cgggatatgg gagttgtatt aagaattata gtaatttaat
     2101 caatagtttg gaccggcggg caggtgctgt cctgatagtg atcggcgggc aataaaaaac
     2161 ccggctcggt ggccggattt gctaaattac tgacctttct taaggtacaa gtttattagg
     2221 aaaaatatag ttggcatagc aagcgaggcc aagagaaccc cgagcatcca tacttttaca
     2281 tctgctatct tggtttccaa agcggaaaat ttagcttcaa agtagtcaac cgatggtttc
     2341 ttagataatg aagcatcaaa atccaggctc ttctgtaaaa gtacagcggt gtctcttttg
     2401 gcgtcagtag aatcagaaga tattttccgg gtatcctctt taattcctgc catgctgctc
     2461 ttgatgtgcg caacttcagc ttcaagaact gctaatcgct tatccatgtc gtcgcctcca
     2521 tcatcacttg ttgtttgaat gatctcagta tcatccatca caaccgatgt gacaatccgt
     2581 ttttctttat ctgaagcagc aacagaacaa gctttcatca agagcgttgc ttggcttgat
     2641 gctacagaag aaaattccag ccctactgta gagccattct tatcagagta agcaattact
     2701 agctttccct catccaaaga tgattgagct gcattcatca gcttttattc ctcgaacatg
     2761 cttttcaagg actgatagaa atcgaaggcc ttctgtttac ttaaagtcac ggacgcaacc
     2821 ttagtgcgct gcattcctgc cactgcgatt tcgccattca tatggccgat gacaggggtg
     2881 ctattgagaa aaacgaagtt aaaaacttcg tagccattgt tatccgttcc gatggttaca
     2941 accccatcag cattagtttc aatataattt ttggactcca gcacatcatt gatatgtgct
     3001 aaggaaactt cttgtttatc gctcatttat gttctcctga accaacaaaa taggtaaaaa
     3061 tcttttcaat ttcattagat taatttgcat aaaaagaaca gggaaaagtt ggattaccag
     3121 ctatgcgccg accagaacac cttgccgatc agccaaacgt ctcttcaggc cactgcgcct
     3181 taactacctt gcctatgatg cggcagctat ggtcgcaatc cagggttcta tatgccgggt
     3241 ttaaaggtac caggtaacta acccctgcat ccttctcata cttcttgaac gttgcctctg
     3301 aatcaccatt tgcagaagcc acgcagaaat ccccagactc taccggctca gccggatcaa
     3361 cgagtatcag catgccttcc ggaaaacttg gacgaacccc ctgaggcgcc gtcattgaat
     3421 gccccttcac ctcaagccag aaagcttttt cgctggcttt tgtggtcgtt gggacccatg
     3481 cctttgcatc gctggctgtg tagcttccca cctccgaaaa tggcccggcc tgcactgaag
     3541 aaaataacgg gtattcatat tggcgaaata cggcgtctga atcctcgcca aacattattt
     3601 ttgccggaga tacgccgagt gccgccccaa gaaccagcgc gtcatccgcg ctaacctttc
     3661 ttgttcctaa ctcgtagttc cccaggcgtg aaggcgcagc ccagccgcaa agcttggcca
     3721 attgagcctg gctaagtcct ttagcttctc taagggactt gatcctttcc ccgataattt
     3781 catgcatcgt tttcatccct taaatgtaac acgcaacgtg attgaactct gtacacgaat
     3841 tgaggttgac tgttaatcac aaattgtgtg taatgggtgt gtgattaatg ctagggagac
     3901 cgcaatgaac aaaattgccc agcagcgaaa aaaaatcgga gtttcgcaag ctgtactagc
     3961 ttcggcaatt ggttgggggc aatcccgcat cgccaactat gagctgaata tccgtactcc
     4021 tggccttaac gattgccgaa tgatcgtaga aggcctcagg aagttagggt gccaatgttc
     4081 tttggatgat gttttccctc catccagtaa caaagccgcc taagcagtac ccgctctttc
     4141 atcatggcct ttcacctcta accgggtgag caaacatcag cggcaaatcc attgggtgtg
     4201 ccgctataac tcaatatcaa tataggaaaa ttaacaaatg gcacaagcaa gctacagcaa
     4261 gccaacacag cgagaaattg atcgcgctga aactgattta ctcatcaacc tgtcaacgct
     4321 tacccagcgc ggtctggcaa agatgattgg ctgtcatgaa tcgaagataa gcagaacgga
     4381 ctggcggttt attgcttcgg tcttgtgtgc tttcggaatg gcatcagaca tcagtccgat
     4441 tagcagggct tttaagtatg cattggatgg aatcacaaag aaaaaatccc cggtggccgc
     4501 cggggactct aagcaaattg atatgcaatt ctgagggaat tactggatca atccacagga
     4561 ttcattatga caaatacagc aaaaatactc aacttcggca gaggtaactt tgccgaacag
     4621 gagcgtaatg tggcagatct cgatgatggt tacgccagac tatcaaatat gctgattgag
     4681 gcttattc
//
LOCUS       DAZKIF010000232         4625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZKIF010000232 DAZKIF010000000
VERSION     DAZKIF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.234"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            14..307
                     /gene="bor"
                     /locus_tag="ACHOTW_004632"
     CDS             14..307
                     /gene="bor"
                     /locus_tag="ACHOTW_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFP9846590.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(398..580)
                     /gene="rzoD"
                     /locus_tag="ACHOTW_004633"
     CDS             complement(398..580)
                     /gene="rzoD"
                     /locus_tag="ACHOTW_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFP9846591.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(797..1273)
                     /locus_tag="ACHOTW_004634"
     CDS             complement(797..1273)
                     /locus_tag="ACHOTW_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP9846592.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(1260..1565)
                     /locus_tag="ACHOTW_004635"
     CDS             complement(1260..1565)
                     /locus_tag="ACHOTW_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9846593.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(1887..2576)
                     /locus_tag="ACHOTW_004636"
     CDS             complement(1887..2576)
                     /locus_tag="ACHOTW_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9846594.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(2573..2713)
                     /locus_tag="ACHOTW_004637"
     CDS             complement(2573..2713)
                     /locus_tag="ACHOTW_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9846595.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(2710..3072)
                     /gene="rusA"
                     /locus_tag="ACHOTW_004638"
     CDS             complement(2710..3072)
                     /gene="rusA"
                     /locus_tag="ACHOTW_004638"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP9846596.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
     gene            complement(3069..3359)
                     /gene="ybcO"
                     /locus_tag="ACHOTW_004639"
     CDS             complement(3069..3359)
                     /gene="ybcO"
                     /locus_tag="ACHOTW_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP9846597.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(3352..3522)
                     /gene="ninE"
                     /locus_tag="ACHOTW_004640"
     CDS             complement(3352..3522)
                     /gene="ninE"
                     /locus_tag="ACHOTW_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9846598.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3522..3977)
                     /gene="ybcN"
                     /locus_tag="ACHOTW_004641"
     CDS             complement(3522..3977)
                     /gene="ybcN"
                     /locus_tag="ACHOTW_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFP9846599.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3974..4072)
                     /locus_tag="ACHOTW_004642"
     CDS             complement(3974..4072)
                     /locus_tag="ACHOTW_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846600.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
     gene            complement(4479..>4625)
                     /locus_tag="ACHOTW_004643"
     CDS             complement(4479..>4625)
                     /locus_tag="ACHOTW_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846601.1"
                     /translation="IIINTDNKTCDIYFMNGIVFKHYPLMKVISAQQAISALKYMVDC
                     EVYF"
BASE COUNT         1164 a         1151 c         1067 g         1243 t
ORIGIN      
        1 cgggaataac accatgaaaa aaatgctact cgctactgcg ctggccctgc ttattacagg
       61 atgtgcccaa cagacgttta ctgttcaaaa caaaccggca gcagtaacac caaaggaaac
      121 catcacccac catttcttcg tctctggaat tgggcagaag aaaactgtcg atgcagctaa
      181 aatttgtggc ggcgcagaaa atgttgttaa aacagaaacc cagcaaacat tcgtaaatgg
      241 attgctcggt tttattactt taggcattta tactccgctg gaagcgcgtg tgtattgctc
      301 acaataattg catgagctgc ccatcgatat gggcagctct atctgcactg ctcattaata
      361 tacttctggg ttccttccag ttgtttttgc atagtgatca gcctctctct gagggtgaaa
      421 taatcccgtt cagcggtgtc tgccagtcgg ggggaggctg cattatccac gccggaggcg
      481 gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg acgaccagcg
      541 gcaacatcat catgcagagc atcattttca gctttcgcat cagctaactc cttcgtgtat
      601 tttgcatcga gcgcagcaac atcacgctga cgcatctgca tgtcagtaat tgccacgttc
      661 gccagcttca gttctctggc atttttgtcg cgctgggctt tgtaggtaat ggcgttatca
      721 cggtaatgat taacagccca tgacaggcag acgatgatgc agatgagcag agcggagata
      781 atcgcggtta ttctgctcat acctcactct ctctgaccgt tccgccagct tctttgaatt
      841 ttgcaatcag gctgtcagcc ttatgctcga actgaccata accagcgccc ggcagtgaag
      901 cccagatatt actgcaacgg tcgattgcct gacggatatc accgcgatca atcataggta
      961 aagcaccacg ctctttaatc tgctgcaatg ccactgcgtc ctggcttttg ggggagaagt
     1021 ctttcaaacc aagctgttta cggtaagcat cccaccagcg tgaaagaagc tgataacgtc
     1081 cggcggctgt tgatttgagt ttggggtgta gcgtgacaag tttgcgaggg tgatcggagt
     1141 aatcagtaaa cagttcgccg ccaacaataa catcataacc gtggttacgt gtcggttgtc
     1201 gcccgttatc cgttccttct gaccacgcca acatatcgag gaaggcttta cgctgaggat
     1261 taagattttg catttttcac ccctgtcagt cgttcccaga agtacgtcag tgcaaccgaa
     1321 cccatcgcac cactaatccc cgctgtcgcg agaatcatgt aaatactgaa tccactttcg
     1381 atgctgatca ggccaccaat aacaccggtg aatcctgata ccactatttg agccagagca
     1441 tttatccaac tccacgttgc tttactctgc ttcacatcta tcaggtagcg gaccagaccg
     1501 ccccaacctg cgatgatcag caaaacgagc cagaacgctc cggcaaggct ctctttttcg
     1561 tgcatatgaa tagccaatgt ttcgccgccg acaaaaggcc gggacgttaa atgtcagaaa
     1621 tcaggctcac ggggtaattt aacgacaaag cacggagttg atgctcccca caagcctgga
     1681 ataaaaaagc cagcatgtag ctggcaacag agggctaagc aatatcaact caacagctga
     1741 agaccccctg gctggggtag gttggaaggc tactcaccgt tcagaaacag aaaagcccaa
     1801 ggctttaaac ctcgggcttg aatttggatt actgccagtg cgtacaacat tggcaaaata
     1861 tcagatttac ataaaatata tgatttttaa tccagttttg caatatcttg ctgtgaaaat
     1921 gtggtctttt gttttgaacg tgttttcgtt aaaagcaata aagcttggct atcaagctgt
     1981 agaaaaatgt gtttcattgc aacccagcgt tcagtaaatg tctcagacca gttttttgat
     2041 gtcactccca ccagtgatgc cagctcctgg tattcatagg tcttacgccc tgccagctcg
     2101 ttcttcacat cctgtgccgc cagccagatc aacttcttca aacgttccag tgtcttacct
     2161 gcaattttcc tggtacccaa cagagtctta aactcgctcc atgcccactg cgttatggtg
     2221 acctgatgtt cccagcgaac actttcgctg taactccaca gcaaccacgc tttctgatgt
     2281 tcttcgagag acagaaccgc gcggcgccat gacgaggttg agaactcaac ctggctgacc
     2341 agtgcaatgg atgaaccttt tgcgtacgac tgcttaccgg aaatcggcgg attatccagc
     2401 gtaatcatcc tgccagttac ctcatccaga atgcgcggct tctttcgttt gtatgtacca
     2461 gtatcaaatt gtgcatgctc cagccaggct tcaagctgtc ctttcgttgc tccgctcaaa
     2521 tcagcggtag ccacaatgag ttgctcgcgg acatactgta aatattgggt attcatgcgg
     2581 cagctccttt cagtgttttg gcgtaattct tcagtatccg gtaatcggtc aaaacagaac
     2641 caggaaaacg atataagcgc aggcgcatcc agcggtggcg aagacgttct gccatataag
     2701 actcaaacat cattcattcc ccagttcggt gatggtcagc tccagcttcc cacctttggt
     2761 aacgggcatc ttcacaacac ggtaatcaac gacctgagca tcatccagcc agaaacctgc
     2821 tttggtgagt gcgtcaaaag cggctttttg cagattatcc aggtcacggc gacggcgatc
     2881 cggcatgtgg cactcaatac ggattttcac tggcatagcc aggccgatat ccagcatgga
     2941 gcctttgatg attcgggcga cgttatcacg gtatgcctgc ccttctgcgc tgatgtgcgt
     3001 gcgcccgcga ttatgccggt agtagcgatt attgctcgga ggccagggta atgtgatgct
     3061 gtaggtattc acgccttaat aaccccctct ttcagccaga taacctgtgt tctcgccata
     3121 ccttccagcg cgcattcttt tgcatatgca gcatcgacaa aatgtgtgcg gcggtcgatt
     3181 tcgtcgtggc aggcagaaca tgcaatggtg gcaatcaagt ctggcggttt ggtaccggtg
     3241 ccgcacaatc cagtcagccg gatatgtgcc agtacagacg tttcagggtt gccattacat
     3301 acgccaggga ttcttacttg gcattcccga ctacgcgctg cttttctcaa atcagccatg
     3361 attcctcctt gctgccagtc gcaaccattt tttatcaacc aggctggcgg tatatccgag
     3421 cagtgttggt atttcggatg gcttcagctc aggtttacgc ttacgacgat ttggtacttt
     3481 gtagatgtgt ccgttcatga cacgaataag cggtgtagcc attacgcctc ctgcttgtca
     3541 cgcagcagct ggaactcgca gctctgtgga atagtcaggt ggcagccaat attcatcgcc
     3601 caggcttcaa ccttacacag gaagacatac atctctccgg taccaagatc ggaggtatgg
     3661 cgtaacgact ggatagtagt gatttcgccg gttacgacat caaccaggtc cttggtttca
     3721 taaccgaggt atgtgtgttt gagagcatct tttacccatg ctgaagtagc gaacgatttc
     3781 cccctgctga tgaggtattc actgatttcg ctgtaccaca tgtggctgag tgcattctgg
     3841 gaaagactgc gtctctcgcg ccacggttta agcaccatgc gaaagcattt gccgtcttcc
     3901 agataagtct ggatctgctg accgacagcg gtgaagttac cgcgatgtaa tttgatgcca
     3961 tcttgtggga ggttcacgct tcacctccgc agagatcaaa cgctggatgc aagacatcgc
     4021 aggtgcattt ctgcatctgt gacaaggtaa ggagttcaga ttgtggtcgc atttaagtcc
     4081 ccttaaatgc gcagaagtca ccggagttgt tcaagctccg gtgacattat tatggaaggt
     4141 tgatactaaa aaatcaaaaa ttgaatatca ttggcgatag tctagcttgt gatatccaca
     4201 agctacttca atgaagcgac gagtaacatt ttgtgtcatc catgtttttt cagttaagtg
     4261 ccacacccag ttcaaaattt gctgatgtgt tgaacatgta ctaagcggga tgttgtattc
     4321 ataagcaaca ttgattccgc aaaatcggtg tcgtgtcatg accgattcac gttgacctgc
     4381 gccctgttga gtgcctgggg atattcttgc tcgatagtta atatcatagt agttacttta
     4441 ctttcatacc atagcacggt tgaaaaagtg attattactc aaaaataaac ctcacaatca
     4501 accatatatt tgagagcact tatcgcctgc tgggcggata ttactttcat taaaggatag
     4561 tgtttaaaaa caatgccatt cataaaatag atatcacagg ttttattatc cgtattaatt
     4621 atgat
//
LOCUS       DAZKIF010000233         4564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZKIF010000233 DAZKIF010000000
VERSION     DAZKIF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.235"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1503)
                     /gene="rhsD"
                     /locus_tag="ACHOTW_004644"
     CDS             complement(<1..1503)
                     /gene="rhsD"
                     /locus_tag="ACHOTW_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFP9846602.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSAVWLTHDPAYPESLPGAPLARYTYTEAGELLAVYD
                     RSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRRAYDEPGR"
     gene            complement(1570..3678)
                     /locus_tag="ACHOTW_004645"
     CDS             complement(1570..3678)
                     /locus_tag="ACHOTW_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9846603.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            complement(4499..>4564)
                     /locus_tag="ACHOTW_004646"
     CDS             complement(4499..>4564)
                     /locus_tag="ACHOTW_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5445 family protein"
                     /protein_id="HFP9846604.1"
                     /translation="DMLGVGDAININTNKNIRQVC"
BASE COUNT          965 a         1367 c         1141 g         1091 t
ORIGIN      
        1 cctgcccggt tcatcatatg cccggcggct ctccagcccg tccggggaca ccaccgccgt
       61 cagctggttc ccgtcgttat agtaaaattt cgtctcccgc ccgtccggtg tggtgatgtc
      121 cgtgatatcg ccggacacca cattcagacc gtactctgtc cgccgtcccg ccgcgtccgt
      181 ctgcgccgtg agccttcctg ccgcgtcata cccgctgtgc gtgacgctgc cgtccgccag
      241 ttcttttttc accacccgct tcagcccggc cccgccttct gtatgcagca cctcacgccg
      301 gttcaggctg tccgtgacgg tgatgcggtc ctgctcatac tggtagcggt aactcaggcc
      361 tgccgggttc agctgctcca ccacccgccc ggtatcgtcg tagcggtagc gcatctccgg
      421 ccttcccgcg taacggtgcg ccaccatccg gcccggatgc tgcgcgtcat acgtgaaagc
      481 gcgcacctgc gtattgctgc ggtcatatac cgccagcagt tcaccggctt ccgtatacgt
      541 gtaccgcgcc agtggcgcac cgggcaggct ttccgggtat gccgggtcgt gcgtcagcca
      601 caccgccgaa aggcggatac ctctgtcggg accgtattcg gtaccgggca gtgtgtcggg
      661 gaacgctgag tctgaaagag ggcggggggt gtcaggggaa gataaagaag cggtgtgctg
      721 tttacgggcc tcttccgccc gctgcgcctg cgtggtcagc accagacgga actcccgccc
      781 ggcaccgtcc gtcacgccgg tgatttcccc ggccaggtca ccggcggcct cacgcctgta
      841 cgtcagcgtc cgcccgaagc ggtccgccat cccggtaagc acccggtacg gcggcagcgg
      901 cgctggcagt acgtcctcag cacccggcac ccgctctgac caccccagta tccaccacgg
      961 cccctgtgcg ctgttggtcg ccaggtaaag atgcgggctt aaccggatat ccggcggcag
     1021 cgccccccac agccgcgcca gcgtgtggcc gtccggctgc gctgccttac caccgcgcac
     1081 cagccacatt gactcgctgc ggctgtacac cgcctccccc ggcagcagcg gctcaaagtg
     1141 aatgctccgc ccgccgttgt cattgagtac cagtgcatca tcgcgtagct gtaagcggat
     1201 atcagaaggc gctttccagc cggggccaaa aatccccacc ggcgcaggcg tccgggtccg
     1261 gtagctgctg taggtgcggg agagaatgaa cggcagcggg ccgggcagcg caaggtccgt
     1321 ctcgccgggc agcaccttcg cccccagcag cggatttacc gggttgcccg aagtcatccc
     1381 gcccgggcac accgagcacg ccacgccagt tggcgcgcca attcttacac ctgccgaacc
     1441 ctggacaatg ggaccgccat actgagtcat atctccctga cgcgccgctg gttttccgct
     1501 catgattcaa ttccttcttc tgtttatttt taatgcttgt ttgttactga ataccgaaca
     1561 ggtacgggat cagtactctc tcatatccgg cgtatcctgc ttttcatcag gggtcttcgg
     1621 tggggatttc gaggctgcac agttccagtt aatcaacagt gagtcaccat taacctgctc
     1681 cttaccctgc aaatgaattt ttgtgccgtt gagaaaaatt tgtccgtcct tcgtcagcac
     1741 cagccttgcc ttaccacagc agagctccag atgctcaccg gcggagtaaa tcgcggtctg
     1801 cccggtatca tcctttttcg ttttaccaac ggtgaaggta acgttatttc cgactttgac
     1861 gtcataaccc agcccgaccc tcaacgattt atgcaaaccg atctgcgagg actgcatcag
     1921 agccaccgaa gtgttcataa tgccacctac cgtcgtctgg tacgccacgc ccacggtcag
     1981 cgcacgaaca agtccaatcg tttccacctg aacagaaccc acgttaatga tctggttact
     2041 gcccaccgtc tctatctggt taacgcccac cgtctgtatc tgattgttgg taaccgcaat
     2101 catctgattg ttgccaatgg tctcagcatg gttgtttatc acatccgtgg tgcggttatt
     2161 cagcacctcg gtgttcatgt tcttctgcgc gtggatgtag acctgttctt tccctgtcgc
     2221 atcgtcaaac ttcagttcat taaacccgct gcccttatag tttttcgaac gaatggtcat
     2281 ctgcgtcttc gtccccggca ggctgccggg tgtgcggttt tcctggtggt aggtacgccc
     2341 cataataatc ggctggtccg gatcgccgtt gaggaagtcc acaatcacct cctgacccac
     2401 acgcggtatc gccaggttgc caaagccggt gcctgcccac gcctgtgcca cacggatcca
     2461 gcatgaactg tcctggtttg acgggttata gcggtcccag ttaaatttca cccgcacgcg
     2521 accatgttcg tcgcagaaga tttcctcgcc tgccggtccc gtcacgacgg cgctctgcgg
     2581 gccatccacc agcggtttca gcaacggctg tggtcgccat gtccggtctg ccggtattac
     2641 cgcaaagtgg ttattcagcg tggtacctga acccctgcgt cccggcaccg cctgtggctg
     2701 ctcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg cctgcggatg
     2761 ccccgtcagc acaattcgcc gtcccggcca tatttccgga gaacggcttg ttccgcgcgc
     2821 cacttctgcg ttgttgcgcc agccatccat ctgccagcgg gcaaagttct gcccgtgggc
     2881 acccttgaaa cgtccggggt agtcatacac ttcatactgt gtgcgctggt aatcctggta
     2941 ctggccttcc tgatcaaaac gccctgccca gccgggtcgt ttaaaggtgt agtccttggt
     3001 caccacggaa gaagggcgga tttgtgcgct gtagcggaac tggctgatgc atagggtgct
     3061 cacctcggta cgggtgttcg ggttccaggg gatctcaaag gactccggca gataacgcac
     3121 ggtgtcgcac aggaccaggc tctggtcgat acttttttgc gcgtgctcct cataaaagaa
     3181 gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcagtttcgc cgtactggac
     3241 acaaaactca cgggaaggat gcggctcgct gaacagcgga ctccactcgg tcacaccgtt
     3301 ttctttcagg atcgtagcga gtatgctttc gatgtcctca ttctggaaga tacggaagtt
     3361 ctggcgcagc cctgtgcgcc acagcggtgg gcacaccttc atgctgtaca gcatctggtt
     3421 tttgtcgttc tcccccagtt caaaccaggt caccacacct ttcacccggc gctgtacgtc
     3481 atcgccctgc cataccgtca ggtaggccat tttgtccagc acctgcgcaa attcaaggga
     3541 aagaaactgc tgactgacca gagaaaggtc gagggaaaaa agtgaagaga gtgactggtt
     3601 cagatgaaag gagaccaccg caaacgcgtc cggtggcagg ccgtccactt ccagcgtgaa
     3661 acgtaatccg gttgacatag caacctccgt gaaaatgaat aataatccgc acatattgca
     3721 tgtacaacat gtcgggcgga tgaaataagt gagtcgacat ctggcgttac cggggaatat
     3781 cagaaaaatg tcatggttat gtgatgattt agtctgggat tttacttcag agaaacgggt
     3841 gtctgtttag tcaacaaaat aatcacaaac gcaacggatg ggttctcgac gttattcacg
     3901 acaggctcgt acattacagc gagtaataaa aaacctgata tgcaaagtca aaccaatatt
     3961 cataatgtct ttttattcac aacaataaat aatttaataa caatcattga aatgaatgat
     4021 gatttgtata aggaatatac gctaccgccc caccctgata acatgatctg cacattgcca
     4081 tcttaacgtc gttaaacact ggcttaatag tggatatttc aaattaggaa tttattaaat
     4141 tcacctgatg aatatcataa gcaaatagca tagaaaataa tgacaactcc cccgagggga
     4201 ccatttccaa agcactgcac caccacctgg tgcccggatg atcttgcttc agtggtaaga
     4261 gtacctgatt cattgttccc aaaatggtgt tttcccctgc ccctccaata atgtgcagct
     4321 ctcacgtgat aatctattga ttaggttata ttaagaatca atatttaaaa tacatccatt
     4381 ttcttgaata aatcaacatg tttattaatc aattgctgtt ttagctctga ctcatcaagc
     4441 tctccatatt tgtcctacag ataattgcga ataaatacag agaacgccat ttatcctttt
     4501 agcatacctg ccggatattt ttatttgtat taatattaat tgcatctcca actccgagca
     4561 tatc
//
LOCUS       DAZKIF010000234         4322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZKIF010000234 DAZKIF010000000
VERSION     DAZKIF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.236"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            85..669
                     /locus_tag="ACHOTW_004647"
     CDS             85..669
                     /locus_tag="ACHOTW_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9846605.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            749..1444
                     /locus_tag="ACHOTW_004648"
     CDS             749..1444
                     /locus_tag="ACHOTW_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9846606.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1473..3989
                     /locus_tag="ACHOTW_004649"
     CDS             1473..3989
                     /locus_tag="ACHOTW_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9846607.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            4000..>4322
                     /locus_tag="ACHOTW_004650"
     CDS             4000..>4322
                     /locus_tag="ACHOTW_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9846608.1"
                     /translation="MKRAPLITGLLLISTSCAYASSEGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKT"
BASE COUNT         1246 a         1008 c          978 g         1090 t
ORIGIN      
        1 ttctacctgt ataatcttta aagatgttga acatatattc acattaaata tgattatgta
       61 cttgttacaa ggataaggtt ataaatgaat aaagttacaa aaacagctat tgcgggctta
      121 cttgcgcttt tcgcaggtaa tgccgctgca accgatggtg aaatagtttt tgatggcgag
      181 attttaaaat ccgcttgtga aatcaatgac tctgataaga aaattgaagt tgctcttggt
      241 cactataatg ccgaacagtt tcgttctgta ggcgatcgta gcccaaaaat tccattcact
      301 attcctttag ttaactgccc agtgactggg tgggagcacg ataacggcaa cgttgaagcg
      361 tctttccgtt tatggctgga aacgcgggat aatggtaccg tccctaactt ccctaacttg
      421 gcaaaagtgg gctcttttgc tggcacagca gcaacaggtg tgggtatccg tattgacgat
      481 gcggaaagcg gaaaccttat gccactaaat gctatgggca atgataacac agtttatcag
      541 atccctgcgg actccgctgg aattgtcaat gttgacctca tcgcttacta cgtatcaacc
      601 gttgaagcat ccgaaatcac cccaggggaa gcagacgctg tcgttaacgt aacgctggat
      661 taccgttaat acgttacggc gttatctgac ctttcagata acgccctttt ccttcctatt
      721 tctcgttgta tcaggttgaa aaataactat gtcaaaacga acattcgcga tgatatttac
      781 cttgttgtgt agcttctgta tcggccaggc gcttgcaggg ggtatcgttt tacagcgaac
      841 gcgagtgatc tatgatgcca gccgcaaaga ggctgcgtta cctgtcgcaa acaaaggcgc
      901 agaaacacct tatttactgc aatcatgggt agataatata gatggtacaa gccgtgcccc
      961 atttattata accccaccgc taattcgtct tgaagctggc gatgattcat cactgcgaat
     1021 tattaaaaca gctgataacc tgcctgaaaa taaagagtcg ctgttctaca ttaatgttcg
     1081 tgccattcca gcaaagaaaa aatcagatga tgttaatgct aacgagttga cgctggtatt
     1141 taaaacacgg atcaaaatgt tttatcgccc cgcacacctg aagggacggg taagcgatgc
     1201 gtggaaatca ctggaattta aacgtagtga tcattcactc aatatatata acccaactga
     1261 atattacgtc gtatttgccg gactagcagt cgataaagcc gatctcacaa gcaaaattga
     1321 atatatcgcg cccggagaac ataaacagtt accacttcca gcatctggcg gaaagaacgt
     1381 gaaatgggct gcgatcaatg attatggcgg cagttccggg acagaaactc gtccactgca
     1441 ataacaaata taaaaacaca ggtcatcagg gaatgccaca acgacaccac cagggacata
     1501 aacgcacacc gaaacagttg gcgctcatta tcaaacgctg tttgccgatg gtgctcactg
     1561 gcagcggcat gctttgcact accgctaacg ccgaagagta ttatttcgac cccattatgc
     1621 tggaaaccac aaaaagtggt atgcaaacaa ccgatctgtc acgtttttca aaaaaatacg
     1681 cacaactacc aggaacttat caggttgata tctggctgaa taaaaagaag gtttcacaga
     1741 aaaaaattac atttaccgcc aatgcagagc aacttctgca gccacagttt acggtagaac
     1801 aactacgtga gctgggtatt aaggtggatg aaatcccggc gctggctgaa aaagatgacg
     1861 atagcgtgat caactcgctt gaacaaatca ttcccggtac agctgctgaa tttgatttca
     1921 atcatcagcg acttaatttg agcattcccc aaattgcact gtaccgtgat gcaagaggtt
     1981 acgtctcccc ttctcgttgg gacgatggta taccaacgct gtttaccaac tactcgttta
     2041 caggttctga taaccgttac cgccagggca atcgtagcca acgacagtac ctaaatatgc
     2101 aaaatggtgc caattttggc ccctggcgat tacgtaacta ttctacgtgg acacgcaacg
     2161 atcaggcgtc aagctggaac actatcagta gttatttaca acgtgatatc aaggcgttga
     2221 agtctcagtt gcttctggga gaaagcgcca ccagcggcag tattttttcc agctacaact
     2281 ttactggcgt gcaactcgct tccgacgata atatgttgcc aaacagccag cgcggatttg
     2341 ccccaacggt acgcggtatc gcaaacagta gtgcaatcgt gactatcagg caaaatggtt
     2401 atgtgatcta tcaaagcaac gtgccagcgg gtgcctttga aattaacgat ctctacccct
     2461 cttccaacag cggcgattta gaagtcacga ttgaagaaag tgacggtacg caacgtcgct
     2521 ttatccagcc ttattcttca ttacccatga tgcagcgacc tgggcatcta aaatatagcg
     2581 cgaccgctgg acgctatcgc gctgatgcaa acagtgatag caaggaaccc gaatttgctg
     2641 aagccacggc aatatatggt ttgaataata cttttacgct gtatggcggc ctgctcggtt
     2701 ctgaagatta ttatgcgctg gggatcggta tcggcggcac acttggcgca ctgggcgcgt
     2761 tgtcgatgga tatcaacaga gctgacaccc aattcgataa ccagcactct tttcatggct
     2821 atcaatggcg tacgcagtac atcaaagata tcccggaaac caacaccaat atcgctgtca
     2881 gctactatcg ctataccaac gatggctatt ttagttttga tgaagccaat acccgcaatt
     2941 gggactataa cagtcgccaa aaaagtgaaa ttcaattcaa catcagccag acaatatttg
     3001 atggggtaag tctgtatgcc tccggttcac agcaagacta ttggggcaat aacgagaaaa
     3061 acaggaatat ctctgttggg gtttccggcc agcaatgggg aattggttac agcctgaatt
     3121 atcaatacag ccgctacact gatcaaaata atgaccgcgc actctctttg aatctcagta
     3181 ttccgttaga acgctggtta ccgcgtagcc gggtttccta tcagatgacc agccagaaag
     3241 atcgcccaac ccaacatgaa atgcgtcttg atggctcact gctggatgat ggtcgcctga
     3301 gctatagtct ggaacaaagt ctggatgacg ataacaacca taacagtagc gtgaacgcca
     3361 gttaccgttc accttatgga accttcagtg ccggatacag ttacggtaat gacagtagcc
     3421 aatacaatta cggcgttacc ggcggcgtgg ttatccatcc tcatggtgtg acgctctcgc
     3481 aatatctggg caacgctttt gcgcttattg atgctaacgg ggcatctggc gtgaggatac
     3541 aaaactatcc ggggattgct actgatccct ttggctatgc agtggttcct tatctcacaa
     3601 cttatcagga aaaccgtctc tcggtagata ctacgcagct gcccgataac gtcgatcttg
     3661 aacaaacaac acagtttgtg gtgcccaaca gaggtgcaat ggtagcggcg cgtttcaacg
     3721 ccaatatcgg ttatcgcgta cttgttacag tcagcgatcg caacggtaaa ccgttgccct
     3781 ttggcgctct tgccagcaac gatgatacgg ggcaacaaag tatcgtcgat gagggcggca
     3841 tactatatct ctctgggata tcgagtaaat cacaaagctg gactgtacgc tggggaaatc
     3901 aggcagatca acaatgtcag tttgctttta gtacaccgga ttcagaacca acaacctctg
     3961 tattacaagg cacagcgcag tgccattaag gataaaaaaa tgaaaagagc gcctcttata
     4021 acaggacttt tgttgatatc cacatcctgc gcttatgcct cctcagaagg gtgtggagct
     4081 gacagcacta gcggtgcgac aaattacagc agtgtggttg atgatgttac ggtgaaccag
     4141 acagataacg tgacaggacg ggagtttacc tctgcaacgc taagtagcac taactggcaa
     4201 tacgcctgtt cctgctctgc gggtaaggca gttaaacttg tctatatggt cagccccgta
     4261 cttaccacca ctggacatca gacaggatat tacaaactca atgacagcct ggatattaaa
     4321 ac
//
LOCUS       DAZKIF010000235         4300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZKIF010000235 DAZKIF010000000
VERSION     DAZKIF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.237"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(112..780)
                     /gene="artM"
                     /locus_tag="ACHOTW_004651"
     CDS             complement(112..780)
                     /gene="artM"
                     /locus_tag="ACHOTW_004651"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP9846609.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(780..1496)
                     /gene="artQ"
                     /locus_tag="ACHOTW_004652"
     CDS             complement(780..1496)
                     /gene="artQ"
                     /locus_tag="ACHOTW_004652"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP9846610.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1503..2234)
                     /gene="artI"
                     /locus_tag="ACHOTW_004653"
     CDS             complement(1503..2234)
                     /gene="artI"
                     /locus_tag="ACHOTW_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP9846611.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2252..2980)
                     /gene="artP"
                     /locus_tag="ACHOTW_004654"
     CDS             complement(2252..2980)
                     /gene="artP"
                     /locus_tag="ACHOTW_004654"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP9846612.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3198..3713)
                     /locus_tag="ACHOTW_004655"
     CDS             complement(3198..3713)
                     /locus_tag="ACHOTW_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9846613.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         1114 a         1080 c         1054 g         1052 t
ORIGIN      
        1 ccggataagg cgtccacgcc gcatccggca caggcaccgt gctgatgtct gatgcgacgc
       61 tggcgcgtct tatcagacct acaaaacccc cggcgaatgt acgcagccac attaattcct
      121 tcgctcaaac gccagcgctt tgcgctcgat cagacgcatc atcagcgtca gcaggccgtt
      181 aacgaccaga taaataatcc ctgccgcgcc gaacaccatg acatcgtagg tgcgtccgta
      241 caacaactgg ctgtatccca tcacttccat cagcgtaatg gtgtaagcca gagaggtact
      301 tttgaacacc agcaccactt cgttggaata agaagagagc gagcgtttaa aggcatacgg
      361 cagcaggatc gccagcgtat cttttttgct cattcccagg gcgctacagg actgccactg
      421 accttccggg atcgcacgaa ttgcaccgta aaacagctgc gtggtatacg ccgcactgtt
      481 cagcgacaac gcaatcagcg cacataacca cggttctgac aacaaatgcc acagtgccgg
      541 atactcctgc aacgtcggga actgacccgg cccgtaataa atcaggaaga tctgcaccag
      601 cagcggtgta ccggtaaaca gcgtgatata accccgcacc agccacacca gcaccggcgt
      661 tttcagcgtc aggatgatgg taaaaatcaa cgccagaatc agtgccacaa tcagcgaggc
      721 aacggttagc gtcaggctgg tgtgcagccc tttcatcagt tcgggtaaat actcaaacat
      781 tagctgggcc tccgctcaaa acgtgtcgcg cgcaggtcaa tgcgtttgag aatgtactga
      841 ctgagcaggg tgatcaccag gtaaatcgcc gccgccacaa tgtaccaggt aaatggttcc
      901 tgggtacgag tagcgatgct tttggtttgc agcattaaat cattcacact aatcaaactg
      961 accagcgcgg tatctttcag cagcaccagc cactgattac cgaggccagg cagcgcatga
     1021 cgccacatct gcggcatcac cagacggaaa aagatagccg atttcgacag ccccagcgcc
     1081 tggccggatt cccactgacc catcggcacc gctttcaacg cgccgcgcag cgtttgcgag
     1141 gcataggcgg catacagcag tgacagagcg atgacaccac aaaggaacgg gctaacgtcg
     1201 aagttttcaa tctgcatctg caccgggatc tctacaatcc caagattgat agtgaagcca
     1261 tccgaaagtg tcagcagcag ctgcgaggag ccaaaataga taaacagcac caccagaatt
     1321 tctggcaggc cacgcagaat ggttaccagc gctgaacctg cccacgcgac aggacgccat
     1381 ttcgcagact cccataccgc aaagaacatc gccagcgcca gcccgacaat caatgcacaa
     1441 acggcaaggc cgacggtcat cccggcggcg cttgctaaag gaaaaaattc attcatcagg
     1501 aattacttct ggaaccattt gttgtagatg gtttcgtaag tgccatcttt cttcactttt
     1561 tccagcgcag tgttgagttt ctgctgcagc tcagtgttgc cctgacgtac cgcgatgccg
     1621 aggccagtac cgaagtaatc tttatcggtc actttatcgc ccactgccgc tagtttcggg
     1681 ttatctttca gccattcagt taccactgcg gtgtcaccaa atacgctgtc gatacgaccg
     1741 ttttgcagat ccagttttgc gttctggtag ctgtcatacg gcacggtggt gatttccggg
     1801 tgcttatcca taatgaattt ctggtgagtc gtaccattct gtacgccgac ttttttgcct
     1861 ttcagctgat caacgctggt gtatttaccc tgctgaccca caaacagggc agagttgtca
     1921 tagtacggcg tggtaaacag tacctgcttt tcacgttccg cggtgatatc catcccggcc
     1981 atcaccgctt ccacgcgacg gaatttcagg cttgggatca ggctgtcaaa tgcctggtta
     2041 gagaaagtac aggtcgcatc aatctcttta cacagcgcct gtgccaggtc gacgtcaaaa
     2101 ccaacgatct ggttgtttgc atcaatcgat tcaaacggag gataggaggc ttcggtagca
     2161 aaacgaatgg tttcggcagc tgtggcggaa agactaaaac ctgcaattaa cgcggcaatc
     2221 agaacttttt tcattgttgt tatcccgaat cttagtgaga gagatagttt ttaaatgctt
     2281 cggtttgcgg ctcggtaaag cagctcgcgt cgccttgttc tacgatatga ccgttttcca
     2341 tatacaccac tcggctggcg gttttacgcg ccacttcaac ttcgtgggtg acgatcacct
     2401 gggtaatatt cgtttctgcc agctcacgaa tgatgctgac gatttgtgcc gtaatttccg
     2461 ggtccagtgc ggcggtcggt tcatcaaaca gcagtacctg cggttccatc attaacgcac
     2521 gggcaatagc aacacgctgc tgctgaccac cagaaagatg cagcgggtaa cgatcgctat
     2581 aaggtttgag acgcagacgt tccagcagtt tttctgcacg ggccagcgcc tgatctttac
     2641 tcaaccccag tacacggcag ggcgcttcaa tcaggttttg ctgcacggtc agatgcggcc
     2701 acaggttgta ttgctgaaac accatgccaa cgttacgacg caaatcgcga atcgctttgt
     2761 ctgagggtgt tttggtgaaa tcgaaatggt tgcctgcaat gttgagcgta ccggagcgcg
     2821 gcatctcaag cagattgagt acacgcagca gcgagctttt acccgcgccg ctggggccaa
     2881 gtaacaccag cgtttcgccc tgtgggcaat ccagcgtgat atcgaacagc gcctgatgcg
     2941 cgccgtagaa gcaattaatg ccgtttaatt gaatactcat tgacactcgt atactggcag
     3001 tctgatagct attgaggtcg aagatagtac ctttgacaga ataattatgc aatatttctg
     3061 ctttaaaagt taaaagcaaa gcgcattatt caataaacat agcacaaaat aacgggggcg
     3121 gtggtcggcg agcataaatg tcggcattcc tcacgaaatg ccggacaatt tacggggttt
     3181 attggttgat caaggcgtta gcgattctcg atggactgac ggagcgtacc cgccgtggca
     3241 tgaacgctac cgcctaagta gcgcacatcg tcgatcaccc aacactggcc ttcctgaatc
     3301 attaacactt catcctgcca gccctggtca ccctgcttga gatccacgcg caatggaatg
     3361 ttacgggcat cacgattagg gatagtcgat gcactggcaa cgtgggcgct gtctggcaag
     3421 gtggttcgac tggagaacgg atcactgctt aacaattcac gatgactatt atcgcggctg
     3481 gcatcgctaa gtagggttgc cagtttgtca ctcaaatagg ggcgcagggc ggtaatgtca
     3541 ttgctgcgat gttgaatgtg gtagtcatag aattgttggg ccacgctgtc aggccccccc
     3601 tggacacagg aaccggtgcg gggaccatta tctttataag ctggcgtgac ggtggtacag
     3661 gcactcagca gcagtgcaca tggaataagt agagatagtg aacggtagcg cataatcatt
     3721 tccttattag cttgctgtct gcgtcacact acgaccaaac gctatcagat tatttaatac
     3781 attgttttta atacaattct gaaacaatct gaacacctga ttgggtttga gtccattgct
     3841 ggcgacccac tcaaggttgc tcgtagctga cttacttaaa ttacccaaac aatagacagt
     3901 gtaaaccatt tgtgaggttg agcgtgttat atggctgaaa tgatcattta aggtaaatat
     3961 gatagttctg aaatggtggt ataaatcctc ctgtagaggt taagagcatc tgcaagaagc
     4021 cagtttcaca tttataacgc gtgataaatg aacgcggtta ttgtctaatc attacaaatt
     4081 tttcacatgc gataagagat cctgctatta agatgattgc caaaattacg ttgtattttt
     4141 gaaaatgagc gaaatttgat agtcccagct ttgacttttg ataatattat ttatgctaca
     4201 gttgtgggta taaaatataa tctttttgaa caagtgaatt tgagctaaga ataaatcaag
     4261 ttgtttcagt gtttcgttaa ttaatatgag tataaaaggt
//
LOCUS       DAZKIF010000236         4249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZKIF010000236 DAZKIF010000000
VERSION     DAZKIF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.238"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..304
                     /locus_tag="ACHOTW_004656"
     CDS             <1..304
                     /locus_tag="ACHOTW_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9846614.1"
                     /translation="DMAFQSKSHTIKKIDAFSNYAQHPTQEGCSYFYDNKYFNSVDML
                     SPIIEKLQKMEINCKIHPYIYIATNDSMSGFYAFTCSQMLNSESPNNCNSLYYKAE"
     gene            309..800
                     /locus_tag="ACHOTW_004657"
     CDS             309..800
                     /locus_tag="ACHOTW_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HFP9846615.1"
                     /translation="MTQRYFLFAGIILCAFIAAILSHIAFHHANEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKIGFIRRYVSFTYKKFRDTVYF
                     TTVKIQKIQKDDNISDEILENITSDFFIKVDTSINFYVTRQNDYNYVFSTGRTPRFIC
                     ISH"
     gene            complement(902..2512)
                     /locus_tag="ACHOTW_004658"
     CDS             complement(902..2512)
                     /locus_tag="ACHOTW_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9846616.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLSFILY
                     FLWQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKNI
                     SPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVAD
                     VLHIKNAISDKFNIVLEITERNLVEDTDHALQKINELRSHGCEFAVDDFGTGYCSLGL
                     LQKLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTSHQAEYLI
                     HNNVTLVQGYLYAKPMKATEFYQWYVNR"
     gene            complement(2610..3969)
                     /locus_tag="ACHOTW_004659"
                     /pseudo
     CDS             complement(2610..3969)
                     /locus_tag="ACHOTW_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
BASE COUNT         1411 a          861 c          683 g         1294 t
ORIGIN      
        1 cgatatggct tttcaaagca aatcccacac tataaaaaaa atagatgctt tctcaaatta
       61 tgctcaacac cctactcagg aaggatgcag ttatttctat gataataaat actttaattc
      121 cgtagacatg ttatcaccga ttattgaaaa attgcagaag atggaaatta actgcaaaat
      181 acatccttac atttacattg caacaaatga tagcatgagc ggattttatg ctttcacctg
      241 tagtcagatg ttaaactctg aatcaccaaa taattgcaac tctctctatt ataaagcgga
      301 gtaatcatat gactcaaaga tattttctgt ttgcgggaat aattttatgt gcttttattg
      361 cagcaattct gagccatatt gcgtttcatc atgccaatga accagcagag caaaacatat
      421 catgtaatgc ccatgttatt aatttcaccg atcatacaaa aatggatcaa tatttttcgt
      481 taaatatgat ttctgaaaat aacacaggaa acatgtatct cacgggcgca tacacggtag
      541 atggaaaaaa gataggcttc atccgccgat atgtgagttt tacatacaag aaattccgtg
      601 ataccgttta cttcaccact gttaaaatac aaaaaataca aaaagatgat aacattagtg
      661 atgaaatcct tgaaaatatc acttctgatt ttttcataaa agttgacacc tcaattaatt
      721 tttatgttac acgtcaaaat gattacaact acgtattctc tacaggcagg acaccacgct
      781 tcatatgtat ttcccactaa cagcattgtt gctcctggcg tttgtcacca gttaataacc
      841 ttctgatggc tttgtttata ttttaaggca ataaaaaacc attccaatat tggttaatta
      901 tttatctgtt tacataccat tgataaaact ctgtcgcttt cattggtttt gcatataagt
      961 atccctgaac taatgtcacg ttattatgaa taagatattc cgcctggtga gaggtactga
     1021 caccttctgc aatcgttatc aaattgagtc gattactcag cttaataata atatctaaaa
     1081 caggcgtttc gtcttccgct gtagtgagcg tgtctataaa gcttttatct attttaagat
     1141 aatcaacaga caacttttgc agaaggccaa gggaacaata tcccgtgcca aaatcatcaa
     1201 ctgcaaattc acagccatgg ctccttaatt catttatttt ctgtaatgca tgatcagtat
     1261 cttcaaccag attgcgttcg gtaatctcaa ggacaatgtt aaatttatca gatattgcat
     1321 tttttatatg taaaacatca gcaacaaaat caggatgtgc aagatgttca gcggcaatat
     1381 ttatcccaag gtgaaacggc tttttcactt tccagctttg tacatcctga ataatcagtt
     1441 caaagagatg cttagttaac ggtataatta aattttcctc ttctgcagcg cggataaaaa
     1501 ctaccggaga tatattttta ccatcttcgc gttgccaacg cattaacgcc tcaaccccca
     1561 gacacgcttt ggttgtcgtg tcacatactg gttgataatg cacagaaaat tctccggatg
     1621 acattccttt cttaatttct tctgccaggg acattttccg agatatccag cgctgccata
     1681 agaagtacag gataaaagaa agaagaatag ataatggaag aatgtaaaat acattactaa
     1741 tgacataggg atataatgaa atcatcggcg tagtaactgt aagactagcc tgactgatcg
     1801 cagaattaaa atcttttttt aacatatata acctgtcatt attatttaca ccactaataa
     1861 ttgcaggccc ttcagtaaag cgaagagaaa atgaatcatc attctcatca tcaagaacat
     1921 ccatcagatc atagatatat cgtgactcta caatactaaa agctgtcata tcattcccta
     1981 ttggcataag gaacactatg gctgttttac ctggtacaaa acttgtaccg ctaattgata
     2041 ctatgcgtga ttctttattt ttattgtgaa agctcgtacc gtaaacatcg ttaacattca
     2101 tttttttgaa accgctaact gaagaacata tttggaaatt attttctata agacccgttg
     2161 ttcgaacata gggggtcaat gctccgttag caatcaattg ttccaggata tctttgcagg
     2221 gtttcatcac cagaatagaa gtaagctttg tcgtatgttt gacaatttgc gtaacattat
     2281 caagctgttt taacagaata ccggcggtaa ttgtcgtatc tcgattcatt tgaaatactg
     2341 acatagcaaa cgacagtaac acccccagaa tgcctggtat tataatgagt acaaacattt
     2401 ttttgttgaa taccatacat gtttccccac gcatcagggt gatgcaacct aagaaattat
     2461 agtacaattg ccagtaaaag cgggtgatag cagactgatc aatgataagc atttaagatc
     2521 atatagtact atgatatttc tgaaaattgt tacgcatgtc tgcgaatagc tatttagcgc
     2581 ttaccaaaat cacccgctaa aatgccgctt tatggtgact cacaaaaatt caccacatcc
     2641 ctcacaacca ccttattacg cccggtctct ttcgcctcgt atagtgcgtt atcagccagt
     2701 cgatagatct cattcgggtt taacgcattt ccctgagtca caacagcgcc aatactaatc
     2761 gtcacttttt gtggtatagc gtattcagga ttatcgccag ttaaacgctc aacattttcc
     2821 cgaatccttt ccgctaaagc tttcgcacgt tcagtatcga tgtccgtcag caagacgcca
     2881 aactcttcgc cgccgacgcg cgctaaaagg tcatctggtc gtatgctttt cccaatgata
     2941 ttcaccactg tttttatcac ctgatcgcca accggatgcc cccaggtgtc gttgactttt
     3001 ttgaagtggt cgatatccat aatcatgacg caataaggcg tttttttggc tgatgctgat
     3061 tgaactgtca gttcattaaa aaaataattt ctgttaaata tattggttaa gggatcgcga
     3121 tgtgcaatgt tctttgttac tcgtagcgca ctgaatatat ggcacataaa tattaccata
     3181 acggttaact tactgactac ttcaatagtg cgactaatat accaagtgga cacgctatac
     3241 ctactcataa ataacagaga gatattgtac agtacggcac aaaaagcaat caccgtcact
     3301 ccattccaga tatcatagcg taaaccatta aagtatagat taaccgaaag caaggttaac
     3361 cacatgaaaa tcattatggt aacatatgag gctttccaaa ccacctgacc attttcattg
     3421 gttaactcgg caatgtgtaa attgtagctc tcataatgac tagaaagtat gtgagcaact
     3481 atcgcccccc ccaaaaacaa aaaacaaact tatgcacaaa gtcatttttt tggaaaataa
     3541 attgcgctgt tttgtgtttt cactaacttt attcaccaat gccagaaata ttaatatgca
     3601 caaactcatc tgacgaaaca aataatagat tgcaatgtca ttttggacaa cactgcttgt
     3661 caaacgcccc tcaataattt gctgaatgat aatgacggtt ttaacaaagt aaatcaaaca
     3721 actcagaaaa gccaaactaa gtatacagtt tgaaaattgc ctacggtcat aaatgaaata
     3781 caactgcata aaaataaatg caacagtatc gataaaaaat aaagcaatca ttaccatcag
     3841 ataggttgat cgtggaacaa cttcctcaac gcctggctga ttaaaccaac tattcaccaa
     3901 aaccaaagca agcccaaaca ataagctcac taatacagta ctactaattc tcaaatagtc
     3961 tttttccata aagctacacc aatctgtagg ttgtagatcc ctttctatta ttaatagata
     4021 taagaactgt actgttgtca atatgaaatt ccgcccacaa acaataccga tgatgatagt
     4081 tgctgaatgt aattattatt gcctaagtgc tttatacatt gtttgcaaca agccaggctt
     4141 gaaaatactt ataaatggtt atagaaaatc cttataaaac attaattcat agatacatta
     4201 ggaataacaa ttaaatccgt gagtgccgta gcgcagcctt tcatcagga
//
LOCUS       DAZKIF010000237         4231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZKIF010000237 DAZKIF010000000
VERSION     DAZKIF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.239"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(78..1004)
                     /locus_tag="ACHOTW_004660"
     CDS             complement(78..1004)
                     /locus_tag="ACHOTW_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9846617.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAVSEMGITLAALSQNLKS
                     LEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            1161..2081
                     /locus_tag="ACHOTW_004661"
     CDS             1161..2081
                     /locus_tag="ACHOTW_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9846618.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            2316..3458
                     /locus_tag="ACHOTW_004662"
     CDS             2316..3458
                     /locus_tag="ACHOTW_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP9846619.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            3932..4032
                     /gene="ykgR"
                     /locus_tag="ACHOTW_004663"
                     /pseudo
     CDS             3932..4032
                     /gene="ykgR"
                     /locus_tag="ACHOTW_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
BASE COUNT         1302 a          927 c          859 g         1143 t
ORIGIN      
        1 tatgtaaacg catcctaatg attacgttat tcctcttacc gatattcgcc cagagcaacc
       61 atcaattaca catatcatca attttcgagt tttactcttt tctccaccat aaaatcaata
      121 aaagcacgaa ctttagaggg cattctcacc cgtgatgaaa cataaatata aaacccagga
      181 aatgaaacag cccaatcctc aagaatcggc tgtaacttgc ccgcattgat atgttcagcg
      241 attgcaaaat ccagatgttg aatgataccc gttccattca gcgccattcg tatcatgctt
      301 tcgtcatcat tagaaatata cttacctgaa acctctattt cgaactcttc attacccccc
      361 acaaccttag aaaactccca tgcagagagt gcaccgctac ttttatgccg caaccgaagg
      421 caattatggt tactcagttc ctgaggcgtt tcaggaatac cgtgttcttt taaataatct
      481 ggcgatgcca cagtaaccag tttaataagc ggagaaacag gaacggcagt catatgctca
      541 tccagtcctt gctcaagacg tatgcccaca tcacagccgt ccgcaataat gttagataaa
      601 ccgtcatcca taatgagttc tatctttgtt ttggggtaac gagtaagaaa ctcgccaata
      661 tgaggctcaa caagcatccg tgctgcaaca cgggaggaat ttattctcaa caggcctgta
      721 ggttcaagtt gggtatcacc aacatctttc aaagcatcat caatttgccc caaagccgac
      781 accagcactt catacagacg ctgtccttcc tcagtaagcg agatattgcg ggtcgtacgg
      841 tatatcagct tggtgttaag acgttcctca agagatttta aattctggga caatgcggcc
      901 agcgtaatac ccatttcaga aacggctctg gaaaaactgc cggcacgaac aatcagcgca
      961 aaccaggcaa gagaaggcaa aattgaaggc tctattttca tcattgtaaa ccttaactta
     1021 acactgtgat agatattaga ccattgatga taaccaatac aagtcatact attacgccgt
     1081 tctcgatatg aacacaaatg ccagttgcgg ataatagaaa tgatatttaa atctgtttag
     1141 gcaaatcccc ggagcttgaa atgaaaactg tttcgataaa acacacttac tgggatatag
     1201 ctgctgatat ctatttccct gacgatttta atagtgaaaa aaagtatcct gccattatca
     1261 gcgcccatcc aattggaagc tgcaaagaac agacatcggg tagtgtgtat ggtgccgccc
     1321 tggcaaaagc tggctttatc gtcattgctt ttgatgctag ttttcaggga agcagcggtg
     1381 gggaaccgcg ttatctggaa gatccaacaa tgcgagtaaa agacttcagc attgtcgttg
     1441 attaccttac cagcttacct tacgttgatg ccgggcgtat tggtgtactg ggtatttgtg
     1501 gtggaggtgg atatgccatt aatgccgcaa tgacagaacg ccgaattaaa gctattggta
     1561 ctgtcacggg tgcaaactac gggcgtctta tgcgtgaggg atttacagcg tttaatccta
     1621 tcggagcact tgaagcaatg gcgcaacaaa gaacagacga agctaacggc gcaaaacttc
     1681 gcgtagatga tttactcccc tcttctccgg atgcagctct tgaagcggga ctaacagaaa
     1741 tagacctttt tgaagccaca gaatattatc gttcgccacg aggctatgca cctaatgggg
     1801 taaatcgttc gctattctca catcagactg ttgccgtggg ttgggatgca tttcatcttg
     1861 cagaagtgct gttaactcaa ccaataatgg ttgtggtggg cgatagagtg ggcgcatttg
     1921 gtgcctaccg tgacggctgc gaaattattg gccgcgctgc ttcaaaacat aaagagctgg
     1981 tcgtggttga aggttattca cattacgacc tgtatgataa gccagaacct gtgaaacagg
     2041 cactcgataa attaattcct ttttataaaa cacatttgta ataattttac agggagatcc
     2101 cttatctccc tgacatttat cctttattga ctacacaaca ataataaaat tattcaaaaa
     2161 gaaatttcat taaaaaacaa aaaagcagca actcaattat tagaaaccct ttaaagggat
     2221 tataaataaa gccagaactc ttggttccaa agctggcaac aactaaccag ttaaaacata
     2281 cgcatcaaaa tcagataaac atttgaggta ttgttatgac aaataaacac ccatcattgt
     2341 tttctccttt tatgctgaca gaaaaaataa agctacgtaa tcgtatcgtt atggctccta
     2401 tgacgacctg gtcagcgaat cctgatggaa ccatctctga gcaagaactc gaattttata
     2461 aacgtcgctc acaaaatgtt ggtttagtaa taacaggatg tacctatgtc acgccaagcg
     2521 gcattgggtt tactcatgaa tttgccgcgt atgacgaccg tttcattaac agccttgaaa
     2581 agttagcagc ggcagccaaa agtggcggtg ctccggctat tttgcagatt ttccacgccg
     2641 gtaataaagc catcccggag cttgtaccaa ataacgatgt tattagtgcc agcgcatcaa
     2701 gcgtaaaatc tggtgacttt atgaagagag tggtacaaag cagggaaatg actgaaaacg
     2761 aaattcagga aaccattaga gcattcggag atgtcacaaa aagggcgatt aaagcaggtt
     2821 ttgatggcat tgaacttcat ggtgcgcacg gattccttct acagaatttt ttctcccctc
     2881 tatttaacca gcgtaatgac cgatggggag gagatctcga aggacgtatg cgcttcccgc
     2941 tggccgtatt acaggaagta aaaaatgtag tctatgaata tgcgacaaag ccctttgcca
     3001 tcggttacag aatatctcct gaagaatctg caacaggggg gctgcggata gaggatactt
     3061 ataaactcct tgatcggctc atttcatccg gtatttcgta tatacatacc tcgctggtca
     3121 gtattaacga tagctaccct gttgagtcgc caaatggccc ccgaactatt gaattgattt
     3181 taaatcatat tgcaggcaga gtgccggtaa tcgctgcagg taaaatacgc acaccgtctc
     3241 aggctcaaga agcaatttcc actggcctgc cacttgttgc gatcggtaaa gggctggtga
     3301 taaatccaga atgggtgact ttagctgaat caggtagagg ccatgaaatt caaacaaccc
     3361 ttaatccaca acttgttcct gaattaacca ttccggataa actatgggat caaattcagg
     3421 catcgaaagg aactggctgg tttccgttaa tggattaaat atctattact ctcccgagag
     3481 taaaactatc aaatcaatga gtaaccgata cagcctcaca aattctttca gataattaac
     3541 ccgtcactgc ggcacgttct ctcataaggc gtgcctgcag tgtaatctag tatgtatccg
     3601 gcatagttga cggtttaata gagctcaatt cctcgcccag gttaccaaca acgatatctt
     3661 gtataacacc ccaggagata cgacattatg atgagatgcg atatgctcct cagcgccaat
     3721 caacgccagg actgggccag tcttgagatc agactcattc ccggaactga cttcaggcga
     3781 catatatcaa tgacgttggg gcaataaaag atgcctgatg taatacatct attcaaattt
     3841 gtgatacatg tcaaaaaatt ggttgaccaa actcgttatt tatataaggg cacttacgaa
     3901 gtgcgctctt ttttaaagcg aggaagtacc aatgaaagag aataaagtac aacaaatcag
     3961 tcataaactg attaatatcg ttgttttgtc gcaattgtag aatacgccta tttatttctc
     4021 catttctatt aataacggca ataaactgtt cacttcagtg atatttaaaa tatgcatcct
     4081 ctcccttttt gtaagtaatt attatatccg tgggagagga atacacgctg tcaggtaatc
     4141 aatcatactg cgataaatca tcggccagta aagtggagat aacctccatt ctcgaaaaat
     4201 ccatactctc agcgaaacta tcatcaatca c
//
LOCUS       DAZKIF010000238         4217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZKIF010000238 DAZKIF010000000
VERSION     DAZKIF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.240"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHOTW_004664"
     CDS             complement(<1..209)
                     /locus_tag="ACHOTW_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9846620.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHOTW_004665"
     tRNA            complement(324..399)
                     /locus_tag="ACHOTW_004665"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHOTW_004666"
     tRNA            complement(406..480)
                     /locus_tag="ACHOTW_004666"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHOTW_004667"
     tRNA            complement(597..681)
                     /locus_tag="ACHOTW_004667"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHOTW_004668"
     tRNA            complement(690..765)
                     /locus_tag="ACHOTW_004668"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHOTW_004669"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHOTW_004669"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9846621.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHOTW_004670"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHOTW_004670"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP9846622.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHOTW_004671"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHOTW_004671"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9846623.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1082 a         1047 c          945 g         1143 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcactat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacgttgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcacctg agtgaaattc gccattcgct aacagcgcga ttaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc aaccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccca
     4201 gtcttccgac cgggcct
//
LOCUS       DAZKIF010000239         4186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZKIF010000239 DAZKIF010000000
VERSION     DAZKIF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.241"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(78..497)
                     /locus_tag="ACHOTW_004672"
     CDS             complement(78..497)
                     /locus_tag="ACHOTW_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9846624.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQIRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            577..831
                     /locus_tag="ACHOTW_004673"
     CDS             577..831
                     /locus_tag="ACHOTW_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9846625.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            828..1250
                     /locus_tag="ACHOTW_004674"
     CDS             828..1250
                     /locus_tag="ACHOTW_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9846626.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETNAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQDALSTAIDAHDDAVQAVYRKAHFSGGGSPSDSVVVH"
     gene            1328..2116
                     /locus_tag="ACHOTW_004675"
     CDS             1328..2116
                     /locus_tag="ACHOTW_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846627.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNAECNARGVTKSAIDRITFMAGFADALIQVGWLVETNGVLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTKTDQKALPEEEKEEDINTYLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFSPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQINTVSFPDSAIPPGFRG"
     gene            2123..2869
                     /locus_tag="ACHOTW_004676"
     CDS             2123..2869
                     /locus_tag="ACHOTW_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9846628.1"
                     /translation="MKNIATGGVLERIRRLAPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELVTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVGQPGIEK"
     gene            2892..3653
                     /locus_tag="ACHOTW_004677"
     CDS             2892..3653
                     /locus_tag="ACHOTW_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9846629.1"
                     /translation="METVFDALKAMGKATSVELATRLDISREEVLNELWELKKAGFVD
                     KSVYTWRVADNNVQQEQPAPAELPEETTMATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PTGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIFDSSSRIVSEK"
     gene            3669..4091
                     /locus_tag="ACHOTW_004678"
     CDS             3669..4091
                     /locus_tag="ACHOTW_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9846630.1"
                     /translation="MAKVFTPEEREKIKGQVVELVRLSGRETLRALEAKTGASRYYIS
                     TLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT         1171 a          914 c         1113 g          988 t
ORIGIN      
        1 attttatgta taaaaaaatg agacgggata aagtgaagca caaacccgga gggggacgct
       61 accggattta tgctggttta agaggctttt tgttttttct ttcgtgctaa ctcttcgtaa
      121 attgcattgt acttctgttt tttctcctca agagttttta aaagttcatc tgtctcactg
      181 tcagggagct cgtccagaag gtcaatgatg attttttgtc ttggatttaa ctcctgatag
      241 aaacgtatct gtccactttc ttctgtatcc tctcccaaaa gataggttgg tgttgttcca
      301 atgagtgttg ctaattccct taatttctct cggcgaggaa ttgtttcacc attaaaccat
      361 ttgctaaccg cttttggtgt taatttcatt cgacgggcaa tttctgcctg ccttccatgt
      421 tgttcataac cagcgttttc acaggctagc gcaagcctac tggcgaactc tttacgcgct
      481 ttatcttcat gaaccataag ttcaatgata ttcgctcttg aatgtactgt cagttctgtt
      541 atagcatgta ctcaaagttc acattgtgag ggtgatatga accagaaaac acttgaagat
      601 gtaatcaaaa ctgttcgcgt ttctgttgtg gccgacgttt gtggtgtcag ccaaagagca
      661 atctacaaat ggatggataa cggtaaattg cctcgcacag aatataccgg cgaaacaaat
      721 tacgctgaaa aaatcgctca tgcatcaaac ggattatttt ctgccgatgc aattttaact
      781 attggcagaa ataaaactac tacgaaaaag ctgatgggag ttgattcatg aaaatcaagc
      841 atgagcacat cgaatcagtg ttgttagccc tggcagccga aaaagggcag gcgtgggtcg
      901 ctaacgcaat tactgaagaa tatctgcgcc aggggggcgg cgaattgccc ctggtaccag
      961 gcaaggactg gaacaatcag cagaatatct atcatcgttg gttgaaaggt gaaacgaatg
     1021 cgcaaaggga aaaaattcag aaactgatcc ctgcggttct ggcaattctt cctcgcgagc
     1081 tgcgtcaccg actctgcatc ttcgataccc tggaacgccg tgcattactg gcggcacagg
     1141 atgcactgag tacggcaatt gatgcgcatg atgatgcagt ccaggccgtt taccgtaaag
     1201 cacatttcag cggcggcggt tctcctagcg attctgtcgt agtgcattga ttgaaattaa
     1261 tcgtaccgaa ctgttttgtt cggtatcagt taaatgtaac gctgcgagcg ttacaaggtg
     1321 aaaacaaatg gcttcaaact ggataaagct cgaagttatt acgccggata agccggaaat
     1381 attcaggctt gctgagattc tgaatattga tccagatgcc gcattaggga aagtcattcg
     1441 cttctgggca tgggcggatc aacaaatgat agacggtaac gcagagtgta acgctcgcgg
     1501 cgttacaaaa agtgcaatag atcgcatcac ttttatggct ggttttgctg atgcgttaat
     1561 tcaggttgga tggctggtcg aaactaatgg tgtgttgtcg cttcctaact ttgagcgcca
     1621 taacgggaaa agctctaaaa aacgggcggt tacaaacgag agagtaacaa aaatacgaga
     1681 actgaaacga aaaggtaacg ctgccagcgt tacaaaaacg gatcaaaaag cgttaccaga
     1741 ggaagagaaa gaggaagata taaatactta tctcccccta aatccccctc gccaaaaacg
     1801 agcgtctaaa aaattcgaac cggaggctat cgagctgcct gactggttgc cggaaacact
     1861 ctggcatgag tgggttcagt tcaggcaggc attgcgaaaa ccgattcgaa cggagcaggg
     1921 cgctaacggg gcgatacggg agctggaaaa attccgccag cagggttttt cacctgagca
     1981 ggtgattcga cacagcatcg ccaatgaata ccagggcttg ttcgcgccga aaggtgttcg
     2041 gccagagacg ttgctccgac agattaacac cgtctcgttt ccggacagtg cgatcccgcc
     2101 aggcttcagg gggtaacaga ccatgaaaaa tattgcgaca ggcggcgttc tggaacgtat
     2161 ccgcagactg gccccgccac atgtaaccgc gccattcagg acggtggcgg agtggcgcga
     2221 gtggcaactt gcagaaggcc agaaacgttg tgaggagatc aaccgtcaga atcgtcagtt
     2281 gcgggtggaa aaaatcctga atcgctccgg catacagccg ttgcaccgca aatgctcgtt
     2341 tgcgaattac caggtgcaga acgacggcca gcgatacgcg ttgagtcagg cgaaatctat
     2401 cgctgacgaa ctggttaccg gatgcacaaa tttcgcgttc agcggaaaac ctggtaccgg
     2461 aaaaaaccat ctggcggcgg ctatcgggaa tcgcctgctg aaagatggcc agacagtgat
     2521 tgtggttacc gtggctgatg ttatgagcgc cctgcacgcc agctatgacg acgggcagtc
     2581 aggcgaaaaa tttttgcggg aactgtgcga agttgatctg ctggttcttg atgaaattgg
     2641 tattcagcgc gagacgaaaa acgagcaggt ggtactgcac cagattgttg atcgccggac
     2701 agcgtcaatg cgcagcgtgg ggatgctgac aaacctgaac tatgaggcca tgaaaacatt
     2761 gctcggcgag cggattatgg atcgcatgac catgaacggc gggcgctggg tgaattttaa
     2821 ctgggagagc tggcgcccga atgttggtca gccaggtatt gagaagtaat ttttaccggg
     2881 aggaaatttt aatggagacc gtttttgacg cactgaaagc gatgggaaaa gccacgtcgg
     2941 tagaactggc tacgcgactt gatatcagtc gagaagaagt gctgaacgag ctgtgggaac
     3001 tgaaaaaggc tggcttcgtt gataaaagcg tatacacctg gcgcgtggct gataacaacg
     3061 ttcagcagga acagccagcg ccggcagagc tgccggaaga aaccaccatg gcaacagtag
     3121 cgaaaatctc agagtgcgat ttaaccgcga cgattgaaca acgtggccca caaacggcgg
     3181 atgagttggc tacattgttc ggtaccacat cacgcaaagt agcttcaacg ctggcaatgg
     3241 ctatcagcaa aggacgcctg attcgcgtaa accagaacgg taaatttcgt tactgcatac
     3301 cgggcgataa tttaccagca gagccgaaag cagcatcggt agcggaaact gatggtaagg
     3361 cctttcctca gcccacaggt gttgcgttac cagtacagga ggctgcaaca caggaagata
     3421 ttaaaacaga aacggtggcg gacattgtgc agtcgctgcc atcgtttact gaaacgcgag
     3481 cggatgacct ggttttacca tcgctgcata tggcaaaccg cgaactgcgt cgggcgaaaa
     3541 atcatgtcca gaagtgggag cgagtctgcg ccgcgctgcg ggagctgaac aagcaccggg
     3601 atattgttcg acagattttc gattcctcca gtcgtattgt gtcggaaaag tgattgccgg
     3661 aggcacctat ggcaaaagta tttacaccag aagagcggga aaaaattaaa gggcaggttg
     3721 ttgaacttgt acgtctgagc ggtcgcgaga cgttacgggc tctggaggct aaaaccggtg
     3781 catcaaggta ttacataagc actctcgcca gagaactggt cgccagtggt gatgtttaca
     3841 attcaggcta cggattattc ccgtctgagc aggcgcgtaa agactggcaa aacgcccgca
     3901 aaaaactatc aagggcaaag gtgaagaaac cggttgtggt tgatccggat cttatctggt
     3961 cattaccaga cggagaaata cgccgctacg acaggcgtct gaacataatc tgtagcgagt
     4021 gccggaagag cgaagctatg cagcgtgtac tggctttcta tcagggtaat tttcagaagg
     4081 tgctgttgtg agccaaatta acaatcggaa cttcgtgaag agaaagcata atccaaatct
     4141 gaataattaa gttcagcact gtaaataaaa tttaatcctt aactgg
//
LOCUS       DAZKIF010000240         4148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZKIF010000240 DAZKIF010000000
VERSION     DAZKIF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.242"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            63..1721
                     /gene="dpiB"
                     /locus_tag="ACHOTW_004679"
     CDS             63..1721
                     /gene="dpiB"
                     /locus_tag="ACHOTW_004679"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP9846631.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1690..2370
                     /gene="dpiA"
                     /locus_tag="ACHOTW_004680"
                     /gene_synonym="citB"
     CDS             1690..2370
                     /gene="dpiA"
                     /locus_tag="ACHOTW_004680"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP9846632.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2411..3796)
                     /gene="dcuC"
                     /locus_tag="ACHOTW_004681"
     CDS             complement(2411..3796)
                     /gene="dcuC"
                     /locus_tag="ACHOTW_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP9846633.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1143 a          962 c         1062 g          981 t
ORIGIN      
        1 ttttgattca acattttaat tacatccgtc aaagaggctc gggacaaccc gcaaggaaaa
       61 caatgttgca gcttaacgag aataaacagt ttgcattttt ccaaagactg gcatttccgc
      121 tgcgtatctt tttgctgatt ctggtgttct caatatttgt cattgcagcc ctggcgcaat
      181 attttacggc cagttttgag gactatttaa cgcttcatgt acgcgacatg gcaatgaatc
      241 aggcgaaaat tattgcctcc aatgacagtg tcatctctga ggtgaaaacg cgtgactaca
      301 aacggctggc gaccatcgct aacaaattac aaagagatac cgattttgat tatgtggtga
      361 ttggggaccg gcactcgatc cgcctttacc atcctaatcc ggagaaaatt ggttatccta
      421 tgcagttcac caaacagggc gcgctggaga aaggggagag ctacttcatt accgggaaag
      481 ggtcaatggg gatggcgatg cgcgccaaaa cgccaatctt tgatgacgat ggaaaagtca
      541 tcggcgtggt gtcgattggc tacctggtga gtaaaatcga tagctggcgg gctgagtttt
      601 tattaccgat ggcaggcgtg tttgtcgtgc tgttagggat tctgatgttg ctgtcgtggt
      661 tcctggcagc gcatatccgt cggcaaatga tgggcatgga gccaaagcaa atcgcacgcg
      721 tggtccgtca gcaagaggcg ctgtttagtt cggtttatga agggctgatt gcggtggatc
      781 cgcatggtta cattaccgcc atcaatcgta acgcaagaaa gatgctgggg ctgagctctc
      841 ccggacggca atggttgggt aaacccattg ctgaagtggt caggcccgcc gatttcttta
      901 ccgaacagat tgatgaaaaa cgtcaggatg tggtggctaa ctttaacggt ctgagcgtta
      961 ttgccaatcg ggaagctatt cgttcaggtg atgatttgct gggggccatt atcagctttc
     1021 gcagtaaaga cgaaatttcc accctcaatg cgcaactgac gcaaataaaa caatacgttg
     1081 agagccttcg tacattgcga cacgagcatc tcaattggat gtcgacgctc aatggtctgt
     1141 tgcagatgaa agagtatgat cgcgtgctgg cgatggtgca gggggagtct caggcccagc
     1201 aacagcttat tgacagcctg cgcgaggcgt ttgccgatcg ccaggtggcg gggctgcttt
     1261 ttggtaaagt gcagcgcgcc cgggaactgg ggctaaaaat gatcattgtc cccggtagcc
     1321 agctttcgca actgccgcca ggactggata gcaccgagtt tgcagccatt gtgggcaatt
     1381 tacttgataa cgccttcgaa gccagcctgc gtagcgatga aggaaacaag atcgttgaat
     1441 tattcctcag cgatgaaggc gatgatgtgg tgattgaagt cgccgatcag ggctgcggcg
     1501 ttccagagtc tctacgagac aaaatatttg agcagggggt cagtacgcgt gctgacgagc
     1561 ccggtgaaca tggcattggg ttgtacttga ttgccagcta cgtaacgcgc tgcggtggtg
     1621 ttatcactct cgaagataat gatccctgcg gtaccttatt ttcaatctat attccgaaag
     1681 tgaaaccaaa tgacagctcc attaacccta ttgatcgttg aggacgaaac gccgctggca
     1741 gagatgcatg ctgaatatat tcgtcacatt cccggattca gtcagatatt actggcggga
     1801 aacctggcgc aggcccgaat gatgatcgag cgttttaagc cggggctaat cttgctcgat
     1861 aactatcttc ctgacggtag agggattaat ttactgcatg aactggtgca ggcgcattat
     1921 cccggcgacg tggtgtttac cactgcagcc agcgatatgg aaacggtgtc tgaagccgta
     1981 cgttgtggtg tatttgatta tctcattaag cccattgcct atgaacggct ggggcaaacg
     2041 ctaacccgtt tccgccagcg taaacatatg ctggaaagta ttgatagcgc cagccagaag
     2101 caaattgatg agatgtttaa tgcctatgct cgcggtgaac ccaaagacga actgccgacc
     2161 ggcattgacc ccttgacgct aaacgcggtg cgaaaactgt ttaaagagcc tggtgtgcaa
     2221 catacggcag aaacggtggc gcaggcactg accatcagtc gcaccactgc caggcgttat
     2281 cttgaatatt gcgccagccg ccatctgatt attgctgaaa ttgttcacgg caaagttggc
     2341 agaccgcaac gcatatacca cagtgggtga catgataaag ccggaggaaa cttgcctccg
     2401 gcattactat ttacttgcct gtgaccgctg ctgccgttcc tggcaccatc agctctgtag
     2461 caacgataac aatcaccaaa ccaacaagca ccggtaccga ggtgcgtttt acgacttcaa
     2521 acggcgagat cttcgccatc ccggcaaccg caacgactac gccagaaacg ggcgaaaggg
     2581 tacggcccag gtttgacgcc tgcagcatcg ggatagtcaa atacgccggg ttaatgccgg
     2641 aagagtgcgc cagtttcggg atcatctcaa caaacgcata aaacggcgca ttgcctgaac
     2701 cggtcgtgac tgccgccagc attgtcagaa tcaccaatac cagcatcagg atgatactcg
     2761 ccgaaccaaa cgaggtagcg atagaaatca gactttgaat aaagccgatg gtgctaagcc
     2821 cctgagcgaa taccccagcg gcaaccagca gcatcaccac gttagcaaac gcatctgcca
     2881 tcccgcgata agccacttcc agaccagaga aaactttctg ggtattaaag ctgcggagga
     2941 actccagaat ggaggcaatc agcatacaaa tcaccagaat agtgatgatg tgtaattgcg
     3001 gaccccattt accgtcaaaa atcagtacac cgatgatcgg cgtgaacggc aaaatggcat
     3061 aaaacgcagg ggcagtggtg gtgatttcac tgacatctaa catttcatga gagatgtgct
     3121 cttttttatc cagataacgt tgccagaaga agtgggcgat cgccatgccg ataattgcag
     3181 caattgagat aggcagcgtc gttttgaagg cgaagtcaat cagcgacatt tcggaagctt
     3241 gcgccgccag caccacatcc cctgaagtcg gtgcgagaat aatcgccgcc ggggaggcac
     3301 aaatggcagc agctgcgcca cgactgatac caacgtttac catcaccgga aatagggttg
     3361 ccatcagcaa aacacccaga ccggttgcgg aagagacggc cagagacatt agacaggcga
     3421 caaaataggc ggcaatcatc agcaggtaag gggagttaat atactgcaat ggttttgacg
     3481 ccagcttgac caccatatca ttcgcgccga tatgggtcat gtaagcggca aatccacaca
     3541 gcatcataat catcatgccg aggtcgccgc cgcggctcat tagtaatatt ttaacgtatt
     3601 caacgatatc cgtggcgctg tagcctgttg aagcctggct ggacggtaac actttgtgcc
     3661 ccataatggc actgataatc agcaataaca ggccaccgac aaataacaca ccagtggcgg
     3721 aatacccttt aatgatgtag cgagctacac ccacaataac cacaacccca ataaggagtt
     3781 caatgaatgt cagcataatt tttcctgtct ccaggcccca aagtaaataa taaaaaattc
     3841 ctaaagctta aggaaaaaat atgcccaata aattggcgat gaatgctgat taaaatcaag
     3901 aaaaactgcc attaagacat tgaagttgct gtttttatac gtagataaca aaacgcatat
     3961 ttagcggttg gcaattatca cataataatg tgtgtaaatt gatggaggat tgatgcaaat
     4021 ttgttaattg caatggtgtt agctgcggtt gtgtgctcaa aaaataatct gcaatgggcg
     4081 tgggttgacg atatataata tattccctgt taagatgatt tttggtatct tattaaaaaa
     4141 taagtcat
//
LOCUS       DAZKIF010000241         4029 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZKIF010000241 DAZKIF010000000
VERSION     DAZKIF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.243"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            90..668
                     /gene="espA"
                     /locus_tag="ACHOTW_004682"
     CDS             90..668
                     /gene="espA"
                     /locus_tag="ACHOTW_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFP9846634.1"
                     /translation="MDTSNATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQA
                     AILMFSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAK
                     LPQDVIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            681..1805
                     /gene="espD"
                     /locus_tag="ACHOTW_004683"
     CDS             681..1805
                     /gene="espD"
                     /locus_tag="ACHOTW_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HFP9846635.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            1826..2764
                     /gene="espB"
                     /locus_tag="ACHOTW_004684"
     CDS             1826..2764
                     /gene="espB"
                     /locus_tag="ACHOTW_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFP9846636.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            2771..3178
                     /gene="cesD2"
                     /locus_tag="ACHOTW_004685"
     CDS             2771..3178
                     /gene="cesD2"
                     /locus_tag="ACHOTW_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFP9846637.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            3214..3435
                     /gene="escF"
                     /locus_tag="ACHOTW_004686"
     CDS             3214..3435
                     /gene="escF"
                     /locus_tag="ACHOTW_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFP9846638.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            3441..3719
                     /gene="escG"
                     /locus_tag="ACHOTW_004687"
     CDS             3441..3719
                     /gene="escG"
                     /locus_tag="ACHOTW_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HFP9846639.1"
                     /translation="MVNDISANKILVWAAVAAANHKLPKYAEAILNVFPQIIPDKKDI
                     AHLEFIILFGLNRKNDAVKALEDCMDDETSQLLYSLVHENGSGWVRGF"
     gene            3804..>4029
                     /locus_tag="ACHOTW_004688"
     CDS             3804..>4029
                     /locus_tag="ACHOTW_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFP9846640.1"
                     /translation="MLNGISNAASTLGRQLVGIASRVSSAGGTGFSVAPQAVRLTPVK
                     VHSPFSPGSSNVNARTIFNVSSQVTSFTPSR"
BASE COUNT         1179 a          749 c          973 g         1128 t
ORIGIN      
        1 agttatcgac tataaggagg aaattatttg atattaatta ctcaataatt tttttgtttt
       61 cctgagaaaa attatcaaga ggtatataga tggatacatc aaatgcaaca tccgttgtta
      121 atgtgagtgc gagttcttcg acatcgacga tctatgactt aggtaatatg tcgaaggatg
      181 aggtggttaa gctatttgag gaactcggtg tttttcaggc tgcgattctc atgttttctt
      241 atatgtatca ggcacaaagt aatctgtcga ttgcaaagtt tgctgatatg aatgaggcat
      301 ctaaagcgtc aaccacggca caaaagatgg ctaatcttgt ggatgccaaa attgctgatg
      361 ttcagagtag cactgataag aatgcgaaag ccaaacttcc tcaagacgtg attgactata
      421 taaacgatcc acgtaatgac ataagtgtaa ctggtattcg tgatcttagt ggtgatttaa
      481 gcgctggtga tctgcaaaca gtgaaggcgg ctatttcagc taaagcgaat aacctgacaa
      541 cggtagtgaa taatagccag ctcgaaattc agcaaatgtc gaatacatta aatctcttaa
      601 cgagtgcacg ttctgatgtg caatctctac aatatagaac tatttcagca atatcccttg
      661 gtaaataacc ggagataact atgcttaacg taaataacga taccctgtct gtaacgtctg
      721 gggttaatac cgcctcgggt acttctggta ttactcaatc tgaaacgggt ttatcgctgg
      781 atttacaact ggttaaatcc atgaactcgt cagcaggctg gacagaaagt agccctttac
      841 cgacgccgcc ggcaggtcac tcattagtga cgccctctgc tgctgaggat gtccttagta
      901 aattgtttgg tggtattagt ggtgaggtta caagtcgcac tgaggaggca gagccacagc
      961 gcacaagcta tccctatctc tctcaggtga ataccgttga ccctcagcaa atgatgatga
     1021 tggtcactct gttatccctg gatacttccg cgcagaaagt ctcgagtctg aaaaactcta
     1081 acgagattta tatggatggg caaactaaag cgctggagaa taaaacgcag gagtataaaa
     1141 aacagctcga agaacaacag aaagccgaag agaaatcaca aaaaagtaaa attgttggcc
     1201 aggtctttgg ttggttgggc gtcgcattaa cagccgttgc cgctgttttt aacccagcac
     1261 tctgggctgt tgttgccatt ggtgcaacag caatggcact gcaaacggca gtcgatgtaa
     1321 tgggggaaaa tgcccctcag ggattaaaga ctgcagcaca ggtctttggc ggaatatcta
     1381 tggccgcaag cattctgaca gccggcgttg gcggggtgtc ttcactgtta tctaaatttg
     1441 gtaatgttgc taacaaaatt ggctcaagcg ttgtaaaagt cgttgagaag gcggcagaag
     1501 cgctggttaa aaacgttttt gcaaaaattt cgacagtggc tgagggcgtt acgaacggta
     1561 ttcgttctgc cgggacaact gcgttgaata atgaggctgc gcaactccaa atgttgtctc
     1621 agttagctgc tttcgcggtg caaaacttaa ctcgacagag tgaaagctta ggtgagagtg
     1681 cgaagctcga gctggataaa gcggcaagcg agttacaaaa tcaggcgagc tatttacaaa
     1741 gtgtttctca actgatgtcc gattcagcac gggtaaatag tcgtattgtt agtggccgaa
     1801 tttaattatt aaagagaatt ttattatgaa tactattgat aatactcaag taacgatggt
     1861 taattccgct tcggagagta cgaccggcgc ttccagtgca gttgccgcat ctgctttatc
     1921 aattgattca tctctgctta ctgatggtaa ggttgatatt tgtaagctga tgctggaaat
     1981 tcaaaaactc ctcggcaaga tggtgactct attgcaggat taccaacaaa aacaattggc
     2041 gcaaagctat cagattcagc aggccgtttt tgagagccag aataaagcta ttgaggaaaa
     2101 aaaagccgcg gcaaccgctg ctttggttgg cgggattatt tcatcagcat tggggatctt
     2161 aggttctttt gcagcaatga acaacgcggc taaaggggct ggtgagattg ctgaaaaagc
     2221 aagctctgca tcttcaaagg ctgctggtgc ggcttctgag gttgcaaata aagctctggt
     2281 caaggctacg gaaagtgttg ctgatgtcgc agaggaggca tccagtgcga tgcagaaagc
     2341 gatggccaca acaacgaaag cagccagccg tgcatctggc gttgcagatg atgttgcgaa
     2401 agcctctgac tttgctgaag atcttgcaga cgccgccgag aagacaagca gaatcaataa
     2461 gttgttgaat tccgtagata aactgaccaa taccacagca tttgttgccg tgaccagtct
     2521 tgctgaaggt acgaaaacgt tgccaacaac aatatctgag tccgtcaaat cgactcatga
     2581 ggttaatgaa caacgtgcga agtcgctgga aaacttccag caggggaatc tggagctgta
     2641 taaacaagac gttcgcagaa cgcaggatga tatcacgact cgtctgcgtg atataacgtc
     2701 cgctgtccgc gatctccttg aggtccagaa tcgtatgggg caatcgggtc gcttagctgg
     2761 gtaattgatc atggtcgata cgtttaatga tgaagtgttt aattactatc ttgaacaaaa
     2821 agggtacaca atacagaagg agtttctttg tggcagtgcc ttttttatcg gatggcggat
     2881 tgagacacct tttttttcat tagcgtacag actggatgaa caagaactga ttttgtgctc
     2941 ttttgaagca cgtaaccaaa cagggcttaa cggccctgtt ttatcgctga ctcatttgct
     3001 cgaagagttg tatcaccatt tttcgggtat taagaaaatc agcgcgatga aatctaagat
     3061 tggttcagat tcagaacgtc aaaagcgcga agagttgttt aattacttca tcagaaaggg
     3121 tgccgtgcag caagaaacag aagacggaat ttggttcgta atgaacgtaa atagttaaaa
     3181 gtttttattt tattaacttc tgagggaaat ttaatgaatt tatctgaaat tactcaacaa
     3241 atgggtgaag taggtaaaac gctgagcgat tctgtgccag agttacttaa tagcaccgat
     3301 ttggttaatg accctgaaaa aatgttagag ttgcagtttg cggttcagca atattctgct
     3361 tatgttaacg tagaaagtgg aatgttgaaa acgataaaag atctggtctc aaccatttct
     3421 aaccgtagtt tttaatttta atggttaatg atatttctgc taataagata ctggtgtggg
     3481 cggctgtagc tgcggcaaac cataagcttc ccaaatatgc ggaagctatc ctgaatgtat
     3541 tcccacaaat tatacctgat aaaaaagata tcgcacattt agaatttatt atcttatttg
     3601 gattaaatag aaaaaatgat gcggtaaagg ctttggagga ctgtatggat gatgagacga
     3661 gtcagttatt gtacagtttg gtccatgaga atggtagtgg ctgggtacga ggattttaat
     3721 ttatattgat ttaaaaatca ttttattatt cgttttgata tatatgagag ttagccaagc
     3781 ttagatataa agaggcataa attatgctta atggaattag taacgctgct tctacactag
     3841 ggcggcagct tgtaggtatc gcaagtcgag tgagctctgc ggggggaact ggattttctg
     3901 tagcccctca ggccgtgcgt cttactccgg tgaaagttca ttcccctttt tctccaggct
     3961 cgtcgaatgt taatgcgaga acgattttta atgtgagcag ccaggtgact tcatttactc
     4021 cctctcgtc
//
LOCUS       DAZKIF010000242         3944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZKIF010000242 DAZKIF010000000
VERSION     DAZKIF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.244"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..198)
                     /locus_tag="ACHOTW_004689"
     CDS             complement(<1..198)
                     /locus_tag="ACHOTW_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP9846641.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGL"
     gene            complement(264..608)
                     /locus_tag="ACHOTW_004690"
     CDS             complement(264..608)
                     /locus_tag="ACHOTW_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP9846642.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(605..1051)
                     /locus_tag="ACHOTW_004691"
     CDS             complement(605..1051)
                     /locus_tag="ACHOTW_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9846643.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(1048..1398)
                     /locus_tag="ACHOTW_004692"
     CDS             complement(1048..1398)
                     /locus_tag="ACHOTW_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9846644.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(1408..1734)
                     /locus_tag="ACHOTW_004693"
     CDS             complement(1408..1734)
                     /locus_tag="ACHOTW_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9846645.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1737..>3944)
                     /locus_tag="ACHOTW_004694"
     CDS             complement(1737..>3944)
                     /locus_tag="ACHOTW_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9846646.1"
                     /translation="IRTPRGQIKELRILDWNRVEPLVADDGEVFYRITPDRNCGITES
                     VTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAAMQGHHIQANSTYFFRNGGRPSGVIE
                     VPGSITEENAKKLKGNWDSGYTGENAGKTAILSNGAKYSPTTFSPVDAQTVEQLKMTA
                     EIVCSVFRVPAYKIGVGHPPSSDNVEALEQQYYSQCLQTLIESIELLLDEALETGENE
                     STEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDALYLQQQNYS
                     LEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRGDTEKMTER
                     ELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVTVDEVRPVL
                     QAMVDDAVGAIPVPRDGRDYDPDVLQQAVNDAVANIPQPADGKSLTPDDVRPMLEQMV
                     KEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAV
                     SHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIP
                     VPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRGTYATHQG
                     GLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLY
                     RGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDAGG"
BASE COUNT          817 a         1303 c          951 g          873 t
ORIGIN      
        1 cagaccattg atgttctcct gctctgtgga gcagagcgtg gtcacatcaa tatcctgttt
       61 ctgcccggcg gtgaactgca cttccttgat ggtgcagtcc aggcgcagat attccgcctt
      121 atccatagtt tcagcagtcg ccggggcaga tgaaatcatc acctgcgtca gctgtgagcg
      181 ttcatacaaa gcagacattc tgcctcctga taataaaaaa cccgcacgcg gcggggtatg
      241 ggttttgtag aaaaaagaaa aagtcacacc gtgacctgaa actccagggt tgcacggtaa
      301 cagcggtttt ccggaatata gtcctgcatt tcactgacgg atcccggggc cagcagcatt
      361 atggcttcac gggcgtcctg acgtatctga cgcgcctgcg tcacagtccc ggcataaacg
      421 tctatctgca ccgacactga ggactccgcc tgcccgccca tcacgtccgc tgacaccgat
      481 gaaatcaggc tgaaaaccac ccacggaagc gccaccgacg gcctgccatc cagcaggggg
      541 accacatacg ggtacacctg cccgccggca agatgcgcca gatgaggata caaatccgcc
      601 tccgtcatcg tctcagtacc tcatcaatgg cccggttcat ccgcgcaatc gccacctgtg
      661 ccgcctgttc actgcgcaca tcaaatgccg ggcgcacaaa cgggtgcggt ggcatattca
      721 cggtccccat ttccacaaac cgccagtaga aagcgttgcg cgggttatcc gccttcatgg
      781 tgttatcgct gttaccggtg tccggattaa caccccggat atgcacaccg gattccatcc
      841 cgccatcgcg ggagcgccgg gaaaggacca ccacattgcg gcgcagtttt cccctgcgta
      901 ccggtgcccg tgacaccact tcttctttca gcacattcgc acccgcacgg gttgcctcac
      961 gcagcacccg gttattttct gcaccactca gaagctgcaa atcgcggctg atgtcctcca
     1021 gccccgaaaa atccagcagg gtttcgatca tttttcccct cccagccgac agagaatttc
     1081 cagacgtccg ccggtcgcat ccggcacggg cagcccgaca acgttcagga tccggtcacg
     1141 ccagggacca ctcagcacat gaagtcgtga cgctgccgtg atttcccgac cggactgacc
     1201 gcgcacccag atgcggattt ccgcctgcgc catttccgca ccggactgca tccgctcccg
     1261 gctgctcctg ccccggatat ccgcatgaat tttcccgcat gacacccatt cttccgtcat
     1321 ttctccggca gcgttacggg ttaacaccgg gttcagaaca cttatcatct gtgtcagacg
     1381 acctgcagat attgccattc cccctcctca taacaccgtc ggacaacgca aatcgtaaat
     1441 cagcacggaa acagaaaacg gcagctcccc ctgaatcagt tcttcccgct ccgcaagatc
     1501 cggattccgg tacagcatcc cggtcagtcg catggcagcc cccttcatcc gggttaatgc
     1561 ctcgcccggg atcagttcac cgtcctcacg aatcacctta tcccggctgc cctgaatgta
     1621 ggccagcagc acggcggtag cctgacgaac cttgtccatc agcatgtcat catccgcgtc
     1681 atggtcgaca cgcagatgtg ccttgatctc ttccagtgtc agtaatgccg tcattttcag
     1741 cctcctgcat cccgcccacg ttttgcagcc agggtccagg ctgatgaatg agcttctccg
     1801 ggtttatctt cggtcatact gttgcagtgc cacagcgagc ccccccacgt caccgtatcg
     1861 ccggggtggt aggtttcacc ggctctgaac acaccgcggt agagcatcac cggcagggaa
     1921 aatgtttttt ccgtacgctg gccactgctc tgccggacca ccacagagaa caaccgctca
     1981 cccgtcatgc tgacgtcaat atccgccacc ccatcaacca ggcattccca tccccgcatc
     2041 ccgtgcgttt tttcatacgc ccgccagagt ccgccctggt gtgtggcata cgtgccccgg
     2101 ggaaaggatt tttgatcgtc aatggcgggg agtatttcca gtgccgtggc atcacgcccg
     2161 tcctgcggag ccggcagggc actcaccgca tccagaaccg ccttctgcag aacatccgga
     2221 tcgtagtcac gaccatcacg cggaacagga atatggctta ccgcctcctt caccatctgt
     2281 tcaagcatcg gacgcacatc atccggggtg agacttttac cgtctgccgg ctgcggaata
     2341 tttgcgaccg catcattcac cgccttctgc agtacttccg gatcatagtc acgaccgtcg
     2401 cgcggaacag ggatatggct taccgcctcc ttcaccatct gttcaagcat cggacgcaca
     2461 tcatccgggg tgagactttt accgtccgcc ggctgcggaa tatttgcgac cgcatcattc
     2521 accgccttct gcagtacttc cggatcgtag tcacgaccat cacgcggaac agggatatgg
     2581 cttacagcct ccttcaccat ctgttcaagc atcggacgca catcatccgg ggtgagactt
     2641 ttaccgtccg ccggctgcgg aatatttgcg accgcatcat tcaccgcctg ctgcagtaca
     2701 tccggatcat aatcacgacc atcacgcggt accggaatgg cccccacagc gtcatccacc
     2761 atcgcctgca gaaccggacg cacctcatcc accgtcacat gcttctgtaa taccgccgac
     2821 agggaagtca gtttctcttc aaacgcttgt gcctgcgagg ccatcttccc ctcaaatgtg
     2881 cgctgtaaat ccgccagcac tgtggaaaat tcttctccca gtgcacgaat aatggacagt
     2941 tcccgttccg tcattttctc agtatccccc ctgaacatcg ctttcaccgc atcatgctct
     3001 gtttcactga ttgccttatt accgtcagat gcgccgtcag gcagctgtga tgaaactgtt
     3061 ttcccggcag acgcgaacgg atcctcacgg gcatcacgac gggacagcgc ctccagactg
     3121 tagttctgct gctgaagata cagtgcatca ccgccggcca ggggcggcag gttctcccgt
     3181 ttacgggcct cattgggcgt gagaagcgta tttttcaccg catcccccag cgttttcatg
     3241 cgccgctcac tgtccattct cagcagcgtg gtgacatcaa attctgtact ctcgttttcc
     3301 cccgtttcca gcgcctcatc cagtaacagt tcaatggact caatcagcgt ctgcagacac
     3361 tgggaataat actgctgctc cagcgcctcc acgttgtcac tggaaggcgg gtggccaacg
     3421 ccaatcttgt aggccgggac acggaacacc gaacagacaa tttcagccgt cattttcagt
     3481 tgttccaccg tctgcgcatc caccggtgaa aacgtcgtgg ggctgtattt tgccccgttg
     3541 ctcagtatgg ccgtcttccc ggcattttcg cccgtatatc cgctgtccca gttccccttc
     3601 agttttttcg cgttttcttc cgtaatactg ccggggacct caatcacgcc tgatggtcgc
     3661 ccgccatttc tgaaaaaata cgtcgaattt gcctgaatat gatgcccctg catggcggcc
     3721 agtcctgcgg catacaccgg cggcagcccc acaagcggat gaaaaaaaca gttaaaacgg
     3781 tcgtggatca cctcccgggc aggcaccgtc accgactctg tgatcccaca gttccggtcc
     3841 ggcgtgatgc ggtagaacac ctcgccgtca tccgccacca gaggttcaac ccggttccag
     3901 tccagaatac gcagttcttt gatctgcccc cggggggtgc ggat
//
LOCUS       DAZKIF010000243         3837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZKIF010000243 DAZKIF010000000
VERSION     DAZKIF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.245"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(15..1499)
                     /locus_tag="ACHOTW_004695"
     CDS             complement(15..1499)
                     /locus_tag="ACHOTW_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9846647.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(1686..2639)
                     /gene="ompT"
                     /locus_tag="ACHOTW_004696"
     CDS             complement(1686..2639)
                     /gene="ompT"
                     /locus_tag="ACHOTW_004696"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP9846648.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            3138..3722
                     /locus_tag="ACHOTW_004697"
     CDS             3138..3722
                     /locus_tag="ACHOTW_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9846649.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(3747..>3837)
                     /locus_tag="ACHOTW_004698"
     CDS             complement(3747..>3837)
                     /locus_tag="ACHOTW_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9846650.1"
                     /translation="QHMGFKLSGRSERDNQGRPYPLLHLYNAM"
BASE COUNT         1041 a          757 c          807 g         1232 t
ORIGIN      
        1 ggaatatttt gaaattattg tatttctgct tcagaatttc ctgggcagta tatattttct
       61 attttttgtg gagtgatcag gtgatgtaca actgctttat ctacacataa attaactccg
      121 gtgagagcta atgtatgccc atcaccattt attgttagta acgggctgga aaatttctct
      181 gccatcttac gggcattaat ccagggcgtt gttgggtcgt atttgtgtgc tacaaacagt
      241 aaaccagagg gcagaacagt atttttcagg cgagttttgt tcaggtcgct atgtattggc
      301 cataattcac aaaaatcagg tgaatcggaa cgtccattgt caaagttaat agccgggaag
      361 gcattcgcaa gagcctcttt tcggaatttt cgctcttctg gtgttaattg ctcatccccc
      421 tgatctacac aaaggattac ccccgaagca tcacttgact cttctgaggc tatcggagca
      481 ctgagcgcag tttcaatttc attactgaca atcccctgag agaactggcg tatggcagtt
      541 gcaagggttg gccatgatga acgccatagc agaaggtctg ttgttaatga tatgagttca
      601 tctgaagata tattttctcc cttactgtct aataaaggtt tgtgatgtaa ttttgataat
      661 agctcatgga actgagttat tgccttatct ctgtctgaag aaagcgggca actttttgta
      721 cgcgcacacc aggatgcaaa gcgatcaaac gtttcctgat aactctgtgc ctgtttgagt
      781 tgccatgtga agttgtcctc caggtcatcg atatcgacga ctccatcaag aacgatagat
      841 cttacgttgt agggaaaacg ttctgcatat aaggctgcaa tttgagttcc atacgaatac
      901 gccacggctg tcagttgttt atcccccaag gcttgcctaa tacgatcaat atcgtataca
      961 gcctcgttag agccgatatg gcgaatgact tcggctccgg tattatggat acaggcatta
     1021 attttattta atacttgttg cttttcggtt atgttttcct gagtctctgt atctgattgc
     1081 cggcagttta ttgtcggagt ggactgcccg acgcctcgag gatcaaatcc aataatatcc
     1141 catgactcac gaagatttgt gactggccag tcaaagttaa tataaggatt tatgcctggt
     1201 aacccgggac caccacttat tatcaggata cttcctttat gcttgctttt tgccggcaat
     1261 tttgtcaacg ctagtttgac ttgtgatttt ttttcataag aagcatctcc gcctgtgtct
     1321 gtatatttta atggaacaga caaataacca catagtaagt caggagacgg tttttcctca
     1381 ccaaaccagt ggttgaattg actggccata caggattgcc actgtatctg ctgggcagat
     1441 acggttactg gtagaagtaa cgttaaaaca actttgaaat gagtaattat ttttcgcatt
     1501 gtgtctctga atatcggaat aaagataaaa tttgaatata ttgaggtctt gtgttgcggt
     1561 aagagattac acgttatgac ataggttaaa tgcttacaaa attagtggat attgtctact
     1621 tgtaactgta aacaaattcc ccggggttat acaataccac cggggagaaa atctggttaa
     1681 cttcgttaaa aggtgtactt aagaccagca gtagtgatga agttatagtt ttctatgcct
     1741 gcaccatttt tgctgtagtc tgaagtgtta tcattgtgat cataaagtga agtattacct
     1801 tttttattcg taacccgatt ccatgtgcct tcaacataaa cttttgcgtt aggtgttacg
     1861 taataacctg cattgactga aacagaatag taattttggt ctttgacttt actgcgataa
     1921 gtgattcttt ttcctgggtc atagtgctca tcgttatcag atgcttccac ccagccgctg
     1981 tatttaaatg tgccacctag ctcaaaatct tcataacgat aacttccagt caagccaatg
     2041 tagggcattt taaaacgttg tttgtagccg attgctcttt ctccattcgg gaaggagccg
     2101 atatcatctc tgaatccctc ctcagaactg tagatatagg aaccacctct ggctgtaaag
     2161 ctataacggc tttcctgata tccggccatg agtcccaggc ggtaattggg ttcgttgagg
     2221 agccagcctt tgatattcag atcaaattcg ttggcataat tgagttgtgt atcagggtgt
     2281 ctactttcat ccgtccaggt tccggggtta ctggaatcca tccagtcctg atcgaccata
     2341 ttgccacctc ggctgccgag agttgtccag ccagcagccc cgatagatat ctggggcatc
     2401 aaatcccaat taattgcacc tttaataatt gcagcgttat tgaatttcca gtcgagttga
     2461 ctgacttttc ggcctccttc ttcggctaga taaacacgct cttttgtttt tccgctcaga
     2521 gttccaagac taatgtccgc atttatgttg tcaggagtaa acgataaagt ctcggtagaa
     2581 gcaaaagagc tgatcgcaat aggggttgtc aggactattc ccagaagttt cgcccgcata
     2641 aaaggtctcc attcaatcgt tttaatgatt gaatatgtat tttttatatc taacttaatg
     2701 agtcaattgt atattgttta actgtttatg gtttgtttaa gtttgaatta ttgtttcgtt
     2761 ttattcattc tttatgggca gcttgccaag tgttgtatta ttgttgtggt ttaatatgct
     2821 ggagattttc tttttgctga tatttttatc tgtctatagt tattcattta tacgcatatt
     2881 agcaaaaaaa atattatttt aggtatttct acagtacatt tgacacgatg attaacctct
     2941 atgtagctag gtttttgtaa agaaactgtg cgtcactgta tccaggactg ttatgtgcca
     3001 aataaggaag ttgaagttac cagctcttac tctaaagcta taacgtatag aaaaaatgca
     3061 tttgagacgt gtaaaatcat ttatttaatc gcaatttatg taatggcata atcaggtcgg
     3121 tgaaagaggg gagaagaatg gataaaagag ctaaaaatca gatagtcgat agcgatattg
     3181 cacgtttgtt actcaagctc agaaagtctc gaaacttgac tgttacagag cttgcccaac
     3241 gatctggtgt cagtcaggcg atgatcagca aagttgaacg aggcacttca agccccagtg
     3301 caacaatact tagtcggtta gctaatgcac tgaatattac gctatcaaag ctttttgcag
     3361 agcttgagat gcaacagaat tcacttgtac ttttggctga ccagcaacaa cattggattg
     3421 atgaagaaac aggcattacc cgctggtctt tgtccccagc gggtgcatgt ccggaactaa
     3481 taaaagttga aatcccccct atggggcaat tgactatccc tgcctctgca aatgagcatc
     3541 tttctggaca aactctatgg atgttaagcg gcagccttga ttttcgagtc aatcatcaga
     3601 ctcatcattt acaagccggt gattgtcttg ctcttacctt tgccagcgag taccaaatga
     3661 gaaacccaga tagcggccag agttgttctt acattgtcgc tttcagccaa aaaaatccat
     3721 gatgtaattc tcctaaaatt atcatgtcac attgcgttat aaagatgtaa tagagggtag
     3781 ggtctgcctt gattgtcccg ctcggaacgt cctgaaagtt taaagcccat atgttga
//
LOCUS       DAZKIF010000244         3760 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZKIF010000244 DAZKIF010000000
VERSION     DAZKIF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.246"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            243..2393
                     /gene="btsT"
                     /locus_tag="ACHOTW_004699"
     CDS             243..2393
                     /gene="btsT"
                     /locus_tag="ACHOTW_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP9846651.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2443..2646
                     /locus_tag="ACHOTW_004700"
     CDS             2443..2646
                     /locus_tag="ACHOTW_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9846652.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2657..3613
                     /gene="yjiA"
                     /locus_tag="ACHOTW_004701"
     CDS             2657..3613
                     /gene="yjiA"
                     /locus_tag="ACHOTW_004701"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP9846653.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3682..>3760)
                     /locus_tag="ACHOTW_004702"
     CDS             complement(3682..>3760)
                     /locus_tag="ACHOTW_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9846654.1"
                     /translation="SEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT          815 a         1025 c         1002 g          918 t
ORIGIN      
        1 aaaatcgctt cggttgtgat catcatcaca taaataacct tcataaacct tgctcaaaac
       61 cgctcaaccg caaaactgac cgcttacgtc cctaaaataa ccactcagtt atttacctta
      121 ctttacgcgc gcgtaactct ggcagcatca ttgcaggata gcggtcaatt tacctcctca
      181 aacgcaacgc aaacctagaa cggcttcggc caactattaa tcaatacatg ccaggtttta
      241 ctatggatac gaaaaaacta ttcaagcaca taccctgggt gattctcgga atcatcggtg
      301 cattctgcct cgcggtagtt gcattacgtc ggggggagca cgtcagcgcc ctgtggatcg
      361 tggtcgcctc tgtgtcggtg tatctggtgg cgtatcgcta ctacagtctg tacatcgccc
      421 agaaggtgat gaaactcgac cccacgcgcg cgacgcctgc ggttattaac aacgacggtc
      481 tgaactacgt tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc gctatcgccg
      541 gtgctggtcc gctggtggga ccggttctcg ccgcgcagat gggctacctg cctggcacgc
      601 tgtggctgct ggcgggggta gtgctggccg gtgcggttca ggactttatg gtgctgttta
      661 tctcctctcg ccgtaacggc gcatctctcg gtgagatgat caaagaagag atggggccag
      721 taccggggac tatcgcgctg tttggctgtt tcttaatcat gatcatcatc ctcgccgtcc
      781 tggcgctgat tgtggttaaa gccctggcag aaagtccgtg gggtgtcttc accgtttgct
      841 caaccgtacc aattgcgctg tttatgggta tctacatgcg ctttatccgt ccggggcgtg
      901 tgggtgaagt ctctgttatt ggtatcgtgc tgctggttgc ctctatctac ttcggtggcg
      961 tgattgctca cgatccgtac tggggtccgg cactgacctt taaagacacc accattacct
     1021 tcgcgctgat tggctatgcg tttgtttccg cgctgctgcc ggtgtggctg atcctcgcac
     1081 cacgcgacta tctggcaacc ttcctgaaga tcggcgttat cgtcggcctg gcgctgggta
     1141 tcgtggtgct gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt gacggtactg
     1201 gcccgctgtg gaaaggcgct ctgttcccgt tcctgttcat caccatcgcc tgtggtgcgg
     1261 tatctggctt ccacgcgctg atctcttccg gtacgacgcc gaaactgctg gctaacgaaa
     1321 ccgacgcgcg tttcatcggc tacggcgcaa tgctgatgga gtccttcgtg gcgattatgg
     1381 cgctggttgc tgcatccatc atcgaaccgg gtctttactt cgcgatgaac accccgcccg
     1441 cgggccttgg catcaccatg cctaacctgc atgaaatggg tggcgagaac gcgccgatca
     1501 tcatggcgca gctgaaagac gttaccgcac acgcggcagc gaccgtcagc tcttggggct
     1561 tcgtgatttc gccagagcag atcctgcaaa ccgcgaaaga cattggtgag ccttctgtcc
     1621 tgaaccgtgc aggtggtgcg ccgacgctgg cggtaggtat cgctcatgtg ttccacaaag
     1681 tgctgccgat ggctgacatg ggcttctggt atcacttcgg tattctgttc gaagccctgt
     1741 tcatcctgac cgcgctggat gcgggtaccc gttctggccg ctttatgctg caagacctgc
     1801 tgggtaactt catcccgttc ctgaaaaaaa ccgactctct ggttgctggt atcatcggta
     1861 ctgcgggctg tgtgggtctg tggggctacc tgctgtatca gggcgtggtc gatccgctgg
     1921 gcggcgttaa gagcctgtgg ccgctgttcg gtatctccaa ccagatgctg gcagccgtag
     1981 cgctggtact gggcaccgtt gtgctgatta agatgaagcg cacccaatac atctgggtaa
     2041 ctgttgttcc ggctgtatgg ctgcttatct gcaccacctg ggcgctgggt ctgaaactgt
     2101 tcagcaccaa cccgcagatg gaaggcttct tctacatggc aagccagtac aaagagaaga
     2161 ttgctaacgg tactgacctt acggcgcagc agattgccaa catgaaccac atcgttgtga
     2221 acaactacac caacgcaggc ctgagtattc tgttcctgat tgtggtgtac agcatcatct
     2281 tctacggttt caaaacctgg cttgcggtac gtaacagcga caaacgtact gacaaagaaa
     2341 caccgtacgt tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac taaccgtgtt
     2401 tagccccgct tcggcggggc tttgttctta tcagagtgaa ctatgtttgg taacttagga
     2461 caggcaaaaa aatatctcgg ccaggcggcg aagatgttga ttggcattcc agactacgac
     2521 aactacgtcg agcatatgaa gaccaaccat cccgacaagc cgtacatgag ctatgaagaa
     2581 ttcttccgcg aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg tatgcgctgt
     2641 tgttaatgga gagagcatga acccgattgc agttacccta ctcaccggtt ttttaggcgc
     2701 gggcaaaacc accctgctgc gccatattct taacgaacaa cacggctaca agattgccgt
     2761 gattgaaaac gaatttggcg aagtctccgt tgatgatcaa ctgattggcg accgcgccac
     2821 gcagatcaaa acgctgacca acggctgcat ctgctgttcg cgctccaacg agctggaaga
     2881 cgcactactc gacctgctgg ataatctcga taagggcaat attcagttcg atcgtctggt
     2941 cattgaatgc accggcatgg ccgatcccgg cccgattatt cagacctttt tctcccatga
     3001 gattttatgc cagcgttacc tgctggacgg cgtgattgcg ctggtggatg cggtacatgc
     3061 cgatgagcag atgaaccagt tcaccatcgc ccagtcccag gttggctacg ccgaccgtat
     3121 tctgctgacc aaaaccgacg tcgcaggcga agcagaaaaa ctgcgcgaac gcctggcgcg
     3181 catcaacgcc cgtgcaccgg tctacaccgt tacccacggc gacatcgacc tgggcctgct
     3241 gttcaacacc aacggtttta tgctggaaga aaacgtcgtc agcaccaaac cgcgtttcca
     3301 ctttatcgcg gataaacaaa acgatatttc gtcgattgtg gtagaactgg attacccggt
     3361 agatatcagc gaagtttccc gcgtgatgga aaacctgctg ctggaatcgg cggataaact
     3421 gctgcgttac aaagggatgc tgtggattga cggcgaacct aaccgcctgc tgttccaggg
     3481 cgtccagcgc ctctacagcg ccgactggga ccgcccgtgg ggcgatgaaa aaccgcatag
     3541 cacaatggtt tttatcggga ttcagttgcc ggaggaagag attcgggcag cgtttgcggg
     3601 gttgaggaag taagttcggg aaagatcaac tttttgagag ttccaccgca acattattac
     3661 gagtggaact ctttagatat gtcagaccaa ccaaagcttc ttcaattgcg ttttcatttt
     3721 ttcatgtctc attttcagtt cagccaatcc ccattctgaa
//
LOCUS       DAZKIF010000245         3700 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZKIF010000245 DAZKIF010000000
VERSION     DAZKIF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.247"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(66..482)
                     /locus_tag="ACHOTW_004703"
                     /pseudo
     CDS             complement(66..482)
                     /locus_tag="ACHOTW_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(482..667)
                     /locus_tag="ACHOTW_004704"
     CDS             complement(482..667)
                     /locus_tag="ACHOTW_004704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846655.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            844..3054
                     /gene="katP"
                     /locus_tag="ACHOTW_004705"
     CDS             844..3054
                     /gene="katP"
                     /locus_tag="ACHOTW_004705"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFP9846656.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            3098..3487
                     /locus_tag="ACHOTW_004706"
     CDS             3098..3487
                     /locus_tag="ACHOTW_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9846657.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            3544..3700
                     /locus_tag="ACHOTW_004707"
                     /pseudo
     CDS             3544..3700
                     /locus_tag="ACHOTW_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3600..>3700
                     /locus_tag="ACHOTW_004707"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1045 a          781 c          952 g          922 t
ORIGIN      
        1 aggagcgaac gtctggttca gcagatttgg cgcaactggc agattgtggt tcgagttcgt
       61 agtcgcacat ctgcctggcg gcagatttcc tgtatcacat ccgcagcaat gcggctcatc
      121 tctgccagta accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac
      181 acaatatgct gccacggaca gtcgggaacc agactcagca gatactgtat ccactgtgcg
      241 ccagccttca ctccgcagtg cgggcaggag cgacttttac accggaagca gacctttttt
      301 atgtggctgc agtccggaga tgaacagcac cactgtgtgt accccatcag cgtggtcccg
      361 caggccatga tttttgtcac agactcaatg actaccggac gcactgaacc ttccggttgt
      421 ttctccagcc agttaagcca gcggtttccc tgctgaaaaa tgtcggcaaa acggggaagc
      481 atcagaaggg cgggggaact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca
      541 tgccgccggc gctgataccg gcaagaatgg tcgcaaactc ccgctccgtg cagcgggcta
      601 tttcaggata cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttctga
      661 catccacaaa gaagggaata ttcaggtctg cgcagcactc aacggcatcg tcagttgcgg
      721 cttggaaccc cttagtattt tttgtctgta gtatctatcc cagcaatagg tatatcctgt
      781 tgcatcaata aagttgactt ttgtatacaa catgcgaatt tcccttaatc cggagctatt
      841 cgtatgataa aaaaaactct tcctgttctg attcttctgg cgctatcggg gagcttttct
      901 accgctgtag ccgctgataa aaaagagact caaaatttct actatccaga aacactggat
      961 ttaactcctc tgagattaca cagccctgaa tcaaatccct ggggggctga ttttgattat
     1021 gccaccagat ttcaacagct ggatatggag gctctgaaaa aagatatcaa agatttgctg
     1081 acaacttccc aggattggtg gcctgcggat tatggtcatt atggtccttt ctttattcgt
     1141 atggcttggc acggtgccgg aacatacagg acatatgatg gccggggagg cgccagtggt
     1201 ggtcagcaac gttttgaacc gctgaacagc tggccggata acgttaatct ggataaagcc
     1261 cgtcgattgc tgtggccagt caagaaaaaa tacggctcca gtatttcctg gggagacctg
     1321 atggtcctga ctggtaatgt tgcccttgaa tccatgggat ttaaaacgct gggatttgct
     1381 ggcggaagag aagatgactg ggagtcggac ctggtatact gggggcctga caacaagcct
     1441 cttgcagata accgggataa aaacgggaaa cttcagaaac ctcttgccgc cacgcagatg
     1501 ggacttattt atgtcaatcc tgaaggcccc ggtggaaaac cagatcctct ggcttccgcg
     1561 aaagatatca gggaagcttt ttcacgtatg gccatggatg atgaggagac tgtggccctg
     1621 atcgcgggag ggcatacatt tggtaaagca catggtgcag cgtctcctga aaaatgtatt
     1681 ggcgcagggc ctgatggtgc acctgtggag gagcagggac tgggatggaa aaataaatgt
     1741 ggtacaggaa acggcaaata taccatcacc agtggcctgg aaggagcctg gtcgacatcg
     1801 ccaacccagt tcacaatgca gtatctgaag aatttatata aatatgaatg ggagctgcac
     1861 aagagtcctg ccggtgctta tcagtggaag cctaaaaaag cggcaaatat agttcaggac
     1921 gcgcatgatc cgtctgtcct gcatccgttg atgatgttta cgacggatat tgctcttaaa
     1981 gttgatcctg aatataagaa aataaccacc cgtttcctga atgatccaaa agcttttgag
     2041 caggcattcg caagagcatg gtttaaactg acccaccggg atatgggacc ggcagcccga
     2101 tatcttggta atgaagttcc tgcagaatca tttatctggc aggatcctct tcctgcggcg
     2161 gattatacaa tgattgatgg taaagacatt aagtcgctga aagagcaggt tatggatttg
     2221 ggtatccctg catctgagct gataaagaca gcctgggctt cagcttccac atttcgtgtg
     2281 actgattatc gtgggggaaa taatggtgcc cgcatcaggt tacagcccga aattaactgg
     2341 gaagttaatg agcctgaaaa actgaagaaa gtactggcat ccctgacctc attacagcgt
     2401 gaatttaaca aaaaacagtc tgacggaaag aaagtgtcgt tggctgattt aattgttctt
     2461 tcgggtaatg ctgcaatcga agatgcggcc agaaaagccg gggtggaact tgagattccc
     2521 tttactccgg gaagaactga cgcctctcag gagcagacgg atgttgcctc attcagtgta
     2581 ctggagccga cagcagatgg attcagaaat tattactcaa aaagcagaag tcatatatcg
     2641 ccggttgaaa gcctcattga taaagccagt cagctggatc tcaccgttcc tgaaatgacg
     2701 gcattactgg gtggtctgcg ggtaatggat attaatacaa ataattcttc gttgggagtg
     2761 tttaccgata cccctggtgt tctggataac aagttttttg ttaatctgct ggatatgtca
     2821 acacgatgga gtaaagcaga taaagaagat acatacaatg gattcgatcg taaaacggga
     2881 gcattaaaat ggaaagcatc ctctgttgat ttaatcttca gttcaaatcc tgaattacgt
     2941 gcggtggcag aagtatatgc ctcggatgat gcgagaaata agtttattca tgattttgtt
     3001 aaatcgtgga ataaagttat gaatagcgat cggtttgatt taaacaataa atgataaaac
     3061 tgatgaggga gggcacctcc ctcatgaagg agaaattatg cagaaaagca ataaagttat
     3121 aatcagctgt ggtatggcat tatatttttt gtgtgggggg attacagtgg cagaagaact
     3181 tgatgaaagc atgcaagttc ttgcaaggaa tatgagtgta cttcagtcat caatggataa
     3241 aaaggagctt cttgaagctc ttgacagaat ggatgatgct gtagatgagt caatgaagcg
     3301 tcttcctgag agcatttcac cggctgatga agaagggaag aatgactata taaaagaact
     3361 tcaaaagctt gaaaaagaga ttatgctggc gaaacaaaaa gtcttatcgg gacagttatc
     3421 agatataccc gattcggtaa acaagatgaa taaaatcaga gttgaggggc atgataaatt
     3481 cagataaatc agttggttga ttcctgcggg acgaatacat acgtcaggaa cataacttta
     3541 tggctggaac gtgaaaatcg tgaactgcgc cgcagtaacg atatccttcg ccaggcttcc
     3601 gcttattttg cgaaggcgga gttcgaccgc ctctggaaaa aataatgcca ctgctggata
     3661 agctgcgtga gcagtacggg gtcggaccgg tatgcagtga
//
LOCUS       DAZKIF010000246         3687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZKIF010000246 DAZKIF010000000
VERSION     DAZKIF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.248"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(156..1313)
                     /gene="intS"
                     /locus_tag="ACHOTW_004708"
     CDS             complement(156..1313)
                     /gene="intS"
                     /locus_tag="ACHOTW_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFP9846658.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(1475..1549)
                     /locus_tag="ACHOTW_004709"
     tRNA            complement(1475..1549)
                     /locus_tag="ACHOTW_004709"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1514..1516),aa:Arg,seq:cct)
     gene            complement(1625..2557)
                     /gene="yfdC"
                     /locus_tag="ACHOTW_004710"
     CDS             complement(1625..2557)
                     /gene="yfdC"
                     /locus_tag="ACHOTW_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9846659.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2851..3606
                     /gene="mlaA"
                     /locus_tag="ACHOTW_004711"
     CDS             2851..3606
                     /gene="mlaA"
                     /locus_tag="ACHOTW_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP9846660.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          883 a          891 c          869 g         1044 t
ORIGIN      
        1 atttatgatt ggttttaatg aaatgcatat gtagttaaca tataataatt gcattttgtt
       61 attgttgatg gtttcttggt gtaagaaatc ctgagaagtg aaaagctttg ctatatgtgc
      121 aaggcttttc ttttttataa gaatcgttgt aataactact ccaccttccc atcaagccag
      181 tccgcccacc actgcatcat ttctctgcgc ttatcgagat actgagcatg gttgtaaatc
      241 ccacgcacag atccgccgtt ggcatgtgct agttgcactt caatagcgtc agcaggccat
      301 tcgtgctcgt tcataatcgt gctgaattca tgcctgaatc cgtgaccgct ttccagacct
      361 tcatagccga tttgtttgat cacaagcagt acagcgttct cacagattga cttcttctta
      421 tcgttgcgcc cggcaaaaac aaactctgat actggtttag tgatggagct tagcgtagtg
      481 agaagttcaa caacctggtc cgacatcgga accacatgaa tcttgcgtcc cttcatcaca
      541 ctggcctcga tggtgataat cctgttttca aaatcgacgt tcttccattg catggaacga
      601 agctctttcg ttcttagtgc tgtgtagcgt aaaactttgg tcgcaatgag agatacgata
      661 cttcctgaaa atgttgcaag tgctttgttg aatgccggga tctggtcggc aggtaaaaac
      721 gggaagttct tcttgcggta tcccttcatg gcttcagcaa ggtcaggtgc cgggttatat
      781 ttagcccttc cggtgacaat agcgtaacgg aaaacctcgc cgcatcttct gcgtgctttg
      841 ttggctcgct ccattgcccc gcgatcttca aatctgcgga ttacttccag cagttgcatc
      901 ggctcaatat cctgaatttc aaggccgccg atgataggta aaatgtcgtc atcaaacatt
      961 tttgcaagtt cagtcgcata ggcggctgac cagacttgcc tcttatgctc gtaccattcc
     1021 ttgtaaatcg cactaaagga attgttgtta gacgaagcct ttttcgcctt taccggatcg
     1081 atgccaaccg agatgtcttt cctcgcagtc catgctttat cccttgcctc ttgcaaagtc
     1141 ataagcggat attttccgac ggtcaggatt ttctccttac cgtcaatctt gtagcgaagc
     1201 tgccatacct ttttccctga tacagggaca taaaggtaca ggccattacc atcgagtagg
     1261 cggtatggtt tttctttcgg ctttgctgct tcaatctgct taacggtgag catgggtaaa
     1321 aatccggtgg gtaaaattat tttatccact ttttacccgt catggcgtgc ggctgtcaac
     1381 gatctgacgc gaaccatgac gaactgtgaa tctacggaag gcttgatatt caggggattt
     1441 tgcggactgg tacggatgga ggcgaactga taaatggtgt cccctgcagg aatcgaacct
     1501 gcaattagcc cttaggaggg gctcgttata tccatttaac taagaggaca atgcggcatg
     1561 agtatacccg ctaatggact gcggggtaag tacgctgccg ctcgattgct taaaccctcg
     1621 ccatttatgc cgggttttta tcatttttct taatgttttc cgcacgttct gctttttggc
     1681 gtgcttctgc tttgcgcttg ttgctcatgt cgttacggat ctgtgcatga ctcattaacg
     1741 cgaagataaa ggtgccgccg cagatgttcc ccgctaaagt aggtagtgcg aagggccaga
     1801 tgaaatcgct ccagtgcagc gtaccgttaa acaccagata gaggatttca acagaaccga
     1861 caacgatgtg agtggtgtca cccagggcaa taagccaggt catcaatata atcaccacaa
     1921 tctttgccgc acccgctgca ggaaacatcc ataccatagt ggcgatcagc cagccggaaa
     1981 tgatcgcgtt ggcaaacatc tcgctggggg tgttcttcat cacatccatg ccgattttga
     2041 caaatgcatc gcgagtttct tcattgaaga ttggcatata ttcaaatgcc cacgccgcaa
     2101 tacctgtccc gagaatatta cccagcagca cgacgcccca taaccgcata agtaagccaa
     2161 cgttgctcat tgtcggtttt tgcatgacgg gtagtaccgc agtcacggtg ttttcggtaa
     2221 ataattgctg gcgggccata atgacgataa taaaaccaaa ggtataaccg agattctcca
     2281 gcaagaagct gcccggcaca ccttccagtt cgacatgaaa tatccctttt gccagtaatg
     2341 aagcgcccat cgacagaccc gccgcaatgg ctgaccacag tagtgccatt gcgtcgcgtt
     2401 ccagctcttt ttcaccatcc tggcggatat gctcatgaat tgccatcgcc cgggagggga
     2461 gtcgatcttc atctatttct atttttttgc cacgctcttt ttcttcgctc tcaacttcaa
     2521 tttcgtcgct gtgttgatca attttgtcgt tgtccatggt ctcttctctt gaattagcac
     2581 gtatagctaa agcgtagcgg cttttttgct cgcaactggc gggagttact ttgaaaatgt
     2641 agaaaaggcc gcgtttgcct ttttctgttt ctatagaatc aagtagccta cagggcggcg
     2701 attaccaggc tatgatcaaa tcagcaaatc agggcgtctg gacatcagtt gacgtgctgt
     2761 tacaatcgcc cacacctaaa caggcggata cggtatcgtt ccgtcatgga tggcaaactg
     2821 cataagccat aaaaaaacag ggagacattt atgaagcttc gcctgtcggc gcttgctctg
     2881 ggaactacgc ttctggtggg gtgtgcgagt tccggtacag atcagcaagg gcgttctgac
     2941 ccgttagaag ggttcaaccg caccatgtac aactttaact tcaatgtatt agacccgtat
     3001 attgttcgac cggtcgctgt cgcctggcgt gattatgttc cgcaaccggc gcgtaacggt
     3061 ttgagcaatt ttaccggcaa ccttgaagaa cctgcggtga tggttaacta cttcttgcag
     3121 ggcgaccctt atcaggggat ggtccacttt acccgctttt tcctgaacac cattttgggg
     3181 atgggcggct ttattgatgt tgcagggatg gcgaacccga aactgcaacg gactgaacct
     3241 caccgcttcg gtagtacgct tggtcattat ggcgtgggtt atggcccgta cgttcaatta
     3301 ccgttctacg gtagcttcac gctgcgtgat gacggtggtg atatggcgga tggtctttac
     3361 ccggttcttt cctggctgac ctggccgatg tctgtgggta aatggacgct tgaaggtatc
     3421 gaaacccgcg cacagctgct ggattccgac ggtctgctgc gtcagtcgtc cgatccttat
     3481 attatggtgc gcgaagcgta cttccagcgt catgatttca tcgctaatgg cggcgaactc
     3541 aaaccgcagg aaaatccgaa cgcgcaagcg attcaggatg atttaaaaga tatcgattct
     3601 gaataagaaa caaataaaaa aggtgagtcg caaaactcac cttttttgtt gttatccctc
     3661 aattaccaca agtaaaagga gataaac
//
LOCUS       DAZKIF010000247         3640 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZKIF010000247 DAZKIF010000000
VERSION     DAZKIF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.249"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            5..1237
                     /locus_tag="ACHOTW_004712"
     CDS             5..1237
                     /locus_tag="ACHOTW_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9846661.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRPLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            1222..1860
                     /locus_tag="ACHOTW_004713"
     CDS             1222..1860
                     /locus_tag="ACHOTW_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFP9846662.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            1939..2208
                     /locus_tag="ACHOTW_004714"
     CDS             1939..2208
                     /locus_tag="ACHOTW_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9846663.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            2229..2873
                     /locus_tag="ACHOTW_004715"
     CDS             2229..2873
                     /locus_tag="ACHOTW_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846664.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            3189..3528
                     /locus_tag="ACHOTW_004716"
                     /pseudo
     CDS             3189..3528
                     /locus_tag="ACHOTW_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          954 a          771 c         1001 g          914 t
ORIGIN      
        1 gaaaatgagt gatacgcttt taaccgaaaa aatccttacc ggagaaaatg ttttgcgtgc
       61 tgcaatcgcc cgcattgagt ggatttttga aacatttccg tcggtatgtc tgtctttttc
      121 aggtggaaag gattccaccg tgcttttcca tcttgtggca gaggtggccc gcaggagaaa
      181 acgtcatttc tctgttctgt tcattgactg ggaggcccag tatcggtgca ccattgaaca
      241 tattcagaag atgcgggaaa tgtaccatga tgtgacggaa accttttact gggtggcact
      301 tcccctgacc acggtaaacg gcgtctcgca gtttcagccg gagtggatat gctgggagcc
      361 aggagtgacc tgggttcgcc agccaccgga agaggccatt acggatatga catattttcc
      421 ctttttccgg tatgccatga cgtttgaaga atttgttccg gcattttctt cctggtttgc
      481 cggtaaccgg tgtggagtgg cagtgctgac cggtgttcgt gcggatgaat ccctcaatcg
      541 ctttatgggg ctggtgtctc agcgcaaact gagatatgcc gatgataagc cctggaccac
      601 ggcatcgcct gaagggtttt attacaccat gtatccgttg tatgactgga aagcccgcga
      661 tatctggata tataacgcca gagcttgtgc catttacaat cctctgtatg acctgatgta
      721 ccgtgccgac gtgccgttac gcaacatgcg tgtctgtgaa ccttttggtc ctgaacaacg
      781 taaggggctg tggctttacc atgttctgga gccggaaacc tgggccagga tgtgtgagcg
      841 ggtgtcaggc gctgccagcg gggcgcttta tgccaatgaa agcggtgcct attttgccct
      901 gcgtaaacgt atatcaaaac cagcccatca tacctggcgc agctatgcga tgttcctgct
      961 ggatgtgatg ccggaaagaa cggcagaaca ttaccgtaat aaaattgctg tctacctgcg
     1021 ctggtatcag acgcggggct tcccggatga catcccggat gaacaggaga atgacctggg
     1081 gagccgggat attccgtcct ggcggcgtat ctgtaagaca cttataaaga atgatttctg
     1141 gtgccggccc ctctccttca gtccgaacaa accccggcac tatgaacgtt atctgcagcg
     1201 tatgaaagaa aggaggaagg aatgggggat tctgtgacac cggagataat tggcaacatg
     1261 attcgtcagt acttcagcca ggaacgacca gaagaagaga ccatacaggc ccttaatcac
     1321 ctgcgcagag tgcttcatga agtcagtcct tttgcacatg agccagtgga ctgtgtgctg
     1381 tgggtaaaag cagatgaggt ggtggccaat gattacaatc ctaatgtcat ggcgccgggt
     1441 gagaagaaac tgctgaagca gtcactggag aaagatggtt tcacacaacc ggtggtggtg
     1501 tctgaggaaa agaaccatta cctggtggtg gatggttttc atcgccaggt gctgggcagg
     1561 gaagcaggta cgggaaaacg tctgaagggc tggctgccgg tggtctgtat taatccggag
     1621 agaaagggac aggcctcacg tattgctgcg accatccgac ataaccgggc caggggcaaa
     1681 catcagataa cctcaatgtc tgatattgtc agggacctgt cgcgcctggg ctggacgaat
     1741 gaacgcattg gtacggagct gggtatggac caggatgaag ttctcagatt gaaacagatt
     1801 agcgggctga cggagctgtt tcaggaagag gatttcagcc cggcatggac ggttcgctga
     1861 gaccgtcaga tatgtatatt atttcagtga gttaatgttt tgagcaggaa aaagagtacc
     1921 ccggaagttg tggaagacat ggttgcgcag aaactggagg ctgctggttg ctggcgcaga
     1981 gcttctgacc gctggctttt tgttatggga aatgttgaat gtacagaagc acaacgggag
     2041 tggttgttgt tgcgccggaa ttattgtctg gcgcagatat cgtccccgcc gctacctgaa
     2101 acgctggata tcagcgaggt ggcgaaagct gcagatgcca cgctgaggcg aatggggatt
     2161 gccactccgt ccggggaggt attcagaaaa ggtacaccgg tctgttgata ttcaggtaac
     2221 aggtattaat gatgaagcaa ggaatggcaa acagtatatc aatgacatgg caacttttat
     2281 gcgatgaaca tggttttacc cgggagcact acaatcagct gaagaagttc agcccggaga
     2341 cattacgtga aattattgct gaaatagcca gctgtcatcc atcaaccagt gtattgctca
     2401 ggaataagtg gctgacaccg ccggaggata ttctggagca gattacccgt gaatacgaac
     2461 gaagaataca aaactgtcca ccgttcagga gcgagaagga agcggaatcc tggttaaatg
     2521 aactatcttt tgctgtgatt gctccgctat accgtgttgc ccccgaacaa acagaacagg
     2581 ttgagtcctt tgttctgcag cttattgccg agcaggagcg ggtttgggag cttattttaa
     2641 ccaatgatgg ttattcatgg cattgcgcac tttatgatat attatttcat ctggttttac
     2701 gaaatatgga agaccagcct gaacatcaga aacagcgaat aacaaaaata ttttatgagc
     2761 ccgatgcaag actggttgca gaagagatta aattccggat tcaatctctg tatgatgatg
     2821 aacaaaagag agctttaagt gaactggtaa atgattttac ttcaaaagaa taaaatgcgg
     2881 cgaggtgatt tttactatat gttaataaaa cggggggtag atgaaagtga atggtgttac
     2941 tacatcaaaa tatgaaggat ggcgagggac gtttcagtta tatagatata aaacggtagt
     3001 tctggtgcac cccaaaggat atctggcagg ggaaatgatt catcgccagg ccctaacgtc
     3061 gtcttgtcaa gattaatgct atagacatag ctaaattttt tatggataag gatatgaact
     3121 gtgcctcagg tccggaacat cctgtaggga ctttttaagt cgggattgag ctgaactaat
     3181 gccaaattat gaacagggaa attcagcagc ggctacagtg ggtaaaactc tatgaaacgt
     3241 ccggtgatgc gggatttgta tgccgtcgct gtggcatttc tcggcccaca ttgcgtaaaa
     3301 tggtggcgca gatatctggc tcagggcatc gctggtctgg aaagccacag ccgtcgcccc
     3361 aagcgttcac catcaacgaa aaccggcact tgcgaagtcg ctctgattct ggagttacgt
     3421 tctcagcgaa atctgggggc aagacgtatt caaagcgagt taaaacgact tcattcaatc
     3481 tcactggcaa tagcaacaat ccacaaagtc cttttccaga atcaggtttg aaccgccccg
     3541 ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc agatcatcgt ttccgatgga
     3601 agcataataa gctttttctg cttctgccgg aggagtatgg
//
LOCUS       DAZKIF010000248         3597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZKIF010000248 DAZKIF010000000
VERSION     DAZKIF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.250"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..700
                     /locus_tag="ACHOTW_004717"
     CDS             <1..700
                     /locus_tag="ACHOTW_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP9846665.1"
                     /translation="WFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFD
                     DAVRRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVN
                     QIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQ
                     LTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRG
                     MAKRIARLAEQSTD"
     gene            799..1812
                     /gene="ybjS"
                     /locus_tag="ACHOTW_004718"
     CDS             799..1812
                     /gene="ybjS"
                     /locus_tag="ACHOTW_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9846666.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1809..2639)
                     /gene="amiD"
                     /locus_tag="ACHOTW_004719"
     CDS             complement(1809..2639)
                     /gene="amiD"
                     /locus_tag="ACHOTW_004719"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP9846667.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2636..2959)
                     /gene="ybjQ"
                     /locus_tag="ACHOTW_004720"
     CDS             complement(2636..2959)
                     /gene="ybjQ"
                     /locus_tag="ACHOTW_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9846668.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3170..>3597)
                     /locus_tag="ACHOTW_004721"
     CDS             complement(3170..>3597)
                     /locus_tag="ACHOTW_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9846669.1"
                     /translation="FHDCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIY
                     NMNAGNNVKVAADAFLEILYSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEIL
                     LLSCFAAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
BASE COUNT          865 a          957 c          890 g          885 t
ORIGIN      
        1 gtggtttctc aatgtgatta cttccgtacc gcccaccacc gccagggcgt tgattcaggg
       61 gctgaaacac gatctgctgg cggatgacac cgcgctacgt gcgctcatcc cacaacggct
      121 gatcgctttc gatgacgcgg tacgtcgcac cctgaaagaa gaagaaaagc tggtcaactc
      181 cagcgactgg ggatacgaca ctcaggcctt tgcccgctgg cgaccagagt atggttattt
      241 tgccaaacag gcgggattta ccgttaaaac gtccgccagc cttgcggctt tatggcaggt
      301 ggtgaaccaa atcggcggta aagagcgtta tttctttggc aatattttgt ggcagacacg
      361 ggcgttgatg gaccgtgcga tcggtcataa attagcgaaa ggccgtccgg agcgcgaata
      421 tttgcaaact ggcgatgcgg tggatagctg gaaagtgatt gtcgttgaac cggaaaaaca
      481 acttacgttg ttatttggca tgaaagcacc ggggctggga cgactgtgtt ttaccctgga
      541 agataaaggc gactatcgta ctatcgatgt ccgcgctttc tggcatccgc acggtatgcc
      601 ggggctgttt tactggttat tgatgatccc cgcgcatctg tttatttttc gcggaatggc
      661 aaaacgaatc gccagactgg cagaacaaag cacagattaa tcgacaaaac ctgcgttatt
      721 ctttcatgtt tcccattgca tcaaggggga aatcacggaa gaatgcgcac gtcattttct
      781 caacaacagt ggattgatat gaaggtactg gttaccggcg ccaccagcgg cttaggtcga
      841 aacgcggtag agtttttatg ccagaaaggc atcagcgtgc gagcgaccgg tcgcaacgag
      901 gcaatgggta aattgctgga aaaaatgggc gcagagtttg ttccggcgga tctgaccgag
      961 ctggtctcat cacaagctaa agtgatgctc gcgggcattg atacgctgtg gcactgctcc
     1021 agctttacct ctccctgggg gacacaacag gctttcgatc tggctaacgt tcgcgccact
     1081 cgccgcctag gtgaatgggc tgtcgcctgg ggtgtacgta actttattca tatctcttct
     1141 ccctccctgt acttcgatta tcaccaccat cgcgatatta aagaagattt tcgccctcac
     1201 cgcttcgcca acgagtttgc ccgcagcaaa gcggccagcg aagaagtgat caatatgctt
     1261 tcgcaggcga atccacaaac gcgctttact attctgcgcc cacaaagtct gttcggaccg
     1321 cacgataaag tctttatccc ccgtcttgcg catatgatgc accactacgg cagcattctg
     1381 ttaccgcatg gcggcagtgc gctggtggat atgacctact atgaaaatgc cgtgcacgcc
     1441 atgtggctgg caagccagga agcctgcgat aagctacctt ccgggcgtgt gtacaacatc
     1501 accaacggcg agcatcgcac actgcgcagc atcgtgcaga agctgatcga cgagttgaat
     1561 attgactgtc gtattcgttc cgtcccttac ccgatgctgg atatgatcgc ccgcagcatg
     1621 gagcgtttag gccgcaagtc agcaaaagag ccgccgctga cccactacgg cgtctcgaag
     1681 cttaattttg actttacgct ggatattacg cgggcacagg aagagttagg ttatcagccg
     1741 gtcatcaccc tggatgaagg tatcgagaaa actgccgcct ggctgcgcga ccacggaaaa
     1801 ctgccgcgct aatcctgccc gtatttctcc agcaacgctt cggcaatcgc ctgagtttct
     1861 gcatccgctt cgccgttata taacgtcggg cggaaatgca tctggaacgc cataatcacg
     1921 cgccgctgct cgcgcggtgt catatcaggt ttaacgtcat aaccgtagcg cgccaaaagc
     1981 tccagcaatg acgcggtatc taccggtgtg tgcggcgcgc gcccggcaag gtaaaagttc
     2041 acccgctgcg catccggcca ggcaccaatc ccctgctgcg ccagttgctg ccagggaaat
     2101 aatggccccg gatcgtcttt gcgctgcggg gcgatatccg catgtgccac tacgttttcc
     2161 ggcttgatgt gataacgggc aataatatct ttcgccagcg gaataagcgc ctgaatctgt
     2221 gccggttcaa acggggcaaa atatttcacc cccgccgatt tttgccagcc acggttttcc
     2281 agctcaatgc caatagaggt gtcgttaagg cgcgttgccc cgcgccaggc gctaatcccc
     2341 gcatgccagg ccagttcttg ttccggcacc aattgccaga tgcgcggttt accgttgtat
     2401 cgcggtggta ccgcagggac cagataatgc gagctgacct gcttatcggt cagtgtcgcc
     2461 agcgagctat caaaatcatc tgcggtgtag tggatcacca gcactttaat gcgcggatac
     2521 gccgcctgcg cctggcgtcg ggtatcaagc tgatatccct ctttctcgac aatgcctttt
     2581 tcgcctgcgc accctgccag taacagagcg acagcgatca gccagaagac tcttctcatc
     2641 gacgcgtttt caccgccgta ccgctgacgc taaccatcag catactgccg ttttgcccga
     2701 ccgtttcgta gtcgatatca ataccgacta cggcatcggc ccccagcgcc cgcgcctggg
     2761 agccaagctc ctcaaaggcg atctcccgcg ctttacgtag ctctttttcg taggcaccgg
     2821 agcgtccgcc aacgatatcg cgaataccag caaagaaatc acggaaaata ttcgcgccta
     2881 aaatcgcttc gccagtcacc acaccgcaat attcaacgat ggtctggcct tccagagttg
     2941 gagttgtgga aaattgcatg atcgatctcc ttgtttgtta agccttcgat ctctaaagca
     3001 ttatcggact gatatgccat gttgggaaga aggttttgat cagtttagga aatcatctca
     3061 ttgtgtcaaa aacagctaca taagcctgta caattatctg tttcgtaacc ggatattggt
     3121 ttcattatgg agcaccgctt acctgcccta ttatgttgtg ccggcaacat taatgagtgg
     3181 gaagagtcaa cttatattgt aaatgattcc taaagtcttc cccatcttgc gcctgaggta
     3241 tataccgctg gattgctttt tggtccgccg ccgcaaaaca agaaagtaat aagatctcaa
     3301 tgcttttgtc attaatataa gcgaagatca ttcttgcaaa ttcgtatata aacgcccgat
     3361 cctgagcgct taatagttta atacaatatt tatttttaag agaataaagt atttcaagaa
     3421 aagcatccgc agcaaccttc acgttattcc cggcgttcat attatatata tctcttgcac
     3481 agatagagta cagagagagt aaacagccat acaaataatc atttattaac tcttttgtcc
     3541 attttactgg caaccatggt tttatcttga caccatgcat gctacagtca tgaaaaa
//
LOCUS       DAZKIF010000249         3549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZKIF010000249 DAZKIF010000000
VERSION     DAZKIF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.251"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            233..1051
                     /locus_tag="ACHOTW_004722"
     CDS             233..1051
                     /locus_tag="ACHOTW_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9846670.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1142..1627
                     /locus_tag="ACHOTW_004723"
     CDS             1142..1627
                     /locus_tag="ACHOTW_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9846671.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1643..2119
                     /locus_tag="ACHOTW_004724"
     CDS             1643..2119
                     /locus_tag="ACHOTW_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9846672.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            2182..2403
                     /locus_tag="ACHOTW_004725"
     CDS             2182..2403
                     /locus_tag="ACHOTW_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9846673.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2566..2940
                     /locus_tag="ACHOTW_004726"
     CDS             2566..2940
                     /locus_tag="ACHOTW_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9846674.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            2987..3361
                     /locus_tag="ACHOTW_004727"
     CDS             2987..3361
                     /locus_tag="ACHOTW_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9846675.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            3358..>3549
                     /locus_tag="ACHOTW_004728"
     CDS             3358..>3549
                     /locus_tag="ACHOTW_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9846676.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFA"
BASE COUNT          823 a         1005 c          904 g          817 t
ORIGIN      
        1 tttaattttg ggggaagata caaaaacaac cagtgaacat atttccggcg ggttatccgc
       61 aatgctgaag gtcagttgca ctggcgatgc cggaatacag cttaatacgt ggttcgccag
      121 tgaagggctt ctcagacaat aaacctcatt catttcaccc atcaggccgc gtcttctccg
      181 ggagacgcgg ctttttcatt tatatcgctt aatcattcat aaggagcaaa tcatgagatt
      241 agccagtcgt tttggttatg cgaaccagat acgtcgtgat cgtccgctga cacacgaaga
      301 actgatgcgc catgtaccga gtatttttgg ggaaaaccga catacctctc gcagtgaaca
      361 ctatgcgtac attcccacta tcaccgtcct ggaaaatctg cagcaggaag gctttcagcc
      421 gttctttgcc tgccagaccc gcgtgcgcga ccagagccgc cgggaataca caaaacacat
      481 gctgcgtctg cggcgggccg gacagataac cggtcagcat gtccctgaaa ttattctgct
      541 caactcccat gacggttcat ccagctacca gatgttaccc ggatattttc gtgccatttg
      601 taccaatgga ctggtctgcg gtcagtctct gggagaattg cgtgttccac accggggaaa
      661 tgtggtggac agagtcattg aaggggctta cgaggtggtg ggcgtttttg accggataga
      721 ggagaagcgt gatgccatgc agtcgctggt cctgccgcca ccggcacgcc aggcgctggc
      781 acaggcggca ctgacttacc gttatggtga cgaacatcag cccgtcacca ccgccgacat
      841 tctgacacca cgacgccggg aggattacgg taaggacctg tggagtgctt atcagaccat
      901 ccaggagaat atgctgaaag gcgggatttc cggtcgcagt gcaaaaggaa aacgtatcca
      961 tacccgggcc attcacagca ttgataccga cattaagctc aatcgcgcat tgtgggtgat
     1021 ggcagaaacg ctgctggaga gcatgcgctg atgccgtttc actgaaagtg cagtcctgac
     1081 tcacatctgt tcctgccccg gggcttcttc cattcattta ctttttataa ggaataacct
     1141 catgacaacc gtttcgcata attccaccac accttctgtt tccgtaacca ctgcatcagg
     1201 gaataaccca cctcagctgg ttgccactcc cgtccctgat gaacagcgca tcagcttctg
     1261 gccgcagcat tttggcctca ttccacagtg ggtgaccctg gagccccgtg tcttcggctg
     1321 gatggaccgt ctttgcgaag actactgcgg tggtatctgg aatctgtaca ccctgaacaa
     1381 cggcggggca tttatggcac ccgaaccgga tgacgatgat gacgaaacat gggtactgtt
     1441 caatgccatg aacggcaacc gcgctgaaat gagcccggaa gccgccggta ttgctgcctg
     1501 tctgatgacg tacagtcatc atgcctgtcg tacggagtgt tatgccatga cggtccacta
     1561 ttaccggctg cgggattacg ccctgcagca tccggaatgc agcgccatta tgcgcatcat
     1621 tgactgaatg gaggcaggga caatgcagca gctttccttt ctgcccggag agatgacgcc
     1681 cggcgagcgc agtctcattc tgcgggccct gcaaaccctg gaccgccatc ttcatgaacc
     1741 cggcgtggcc ttcacctcca cccgtgcggc acgggaatgg ctgattctga acatggcggg
     1801 actggagcgg gaagagttcc gggtgctgta tctgaacaac cagaatcagc tgattgccgg
     1861 tgaaaccctc ttcaccggca ccatcaaccg tacggaagtt catccccggg aagtgattaa
     1921 acgcgccctg taccacaatg ccgctgccgt ggtgctggcg cacaatcacc cgtccggtga
     1981 agtcacaccc agtaaggcag accggcttat caccgaacgt ctggtacagg cactgggcct
     2041 agtggatatc cgggtgccgg accatctgat agtcggtggc aaccaggttt tctcctttgc
     2101 cgaacatggt ctgctttaac ccgtcacaac cacatcacac ctgttttcac ttttatcttc
     2161 tgtcttcaga ggtatcacat catgagaatc atcacccgtg gtgaagccat gcgtattcac
     2221 caacagcatc ctgcatcccg tctttttccg ttctgtaccg gtaagtatcg ctggcacggc
     2281 agtgctgaag cgtatactgg tcgtgaagtg caggatattc ccggcgttct tgccgtgttt
     2341 gcagaacgcc gtaaggacag ttttggcccg tatgtccggc tgatgagtgt caccctgaac
     2401 tgatgttccg tcacccggga aacgagtacg accagatgac ctcagacgcc gctgtggatg
     2461 aggccattct catcaatgca tacatcttca ccgaaaccgg tcagcgcgcc ggctgacctg
     2521 tcatcgttaa accattctta accacttttc agagaggatt ttatcgtgtc agacaaactc
     2581 tccgggataa ctcatcccga tgacaatcac gaccgcccct ggtgggggct tccctgcaca
     2641 gtgaggccct gttttggcgc tcgtctggtg caagagggta accgcctgca ttaccttgcc
     2701 gaccgcgccg gtatcagagg ccgcttcagc gacgcagatg cataccatct ggaccaggcc
     2761 tttccgctgc tgatgaaaca actggaactc atgctcacca gcggtgaact gaatccccga
     2821 tatcagcata ccgtcacgct gaatgcaaaa gggctgacct gcgaagccga caccctcggc
     2881 tcctgtggtt acgtttatct ggctgtttat ccgacaccag cacccgcaac cacctcataa
     2941 ccccgaagct ctcaccttta gcactaccag ttacagagaa cttaagatga aaacattatc
     3001 cgacacacat gtacgggagg tatctcgctg cccgtctccc gtcaccatct ggcagacact
     3061 gctcatccga ctgctggacc agcattatgg cctcacactg aatgacacac cgtttgccga
     3121 tgaacgtgtg attgagcagc atattgaggc aggcatttca ctgtgtgatg cagttaattt
     3181 tctcgtggaa aaatacgcgc tggtgcgtac cgaccagccg ggattcagca cctgtccccg
     3241 ctctcagtta ataaacagta ttgatatcct ccgggctcgc cgggcgaccg gcctgatgac
     3301 ccgcgacaat tacagaacgg taaataacat taccctgggt aagtatccgg aggcgaaatg
     3361 atgaaactgg ccctgacact ggaagccgac agcgttaacg tacaggcact gaacatgggg
     3421 cgcattgtcg ttgacgtcga tggtattgag ctcgctgaac tgattaacat ggtctgcgat
     3481 aacggctact cccttcgtgt tgttgatgaa tctgaccaga cctcagcaga atgcacgcca
     3541 ccatttgct
//
LOCUS       DAZKIF010000250         3546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZKIF010000250 DAZKIF010000000
VERSION     DAZKIF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.252"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..900)
                     /locus_tag="ACHOTW_004729"
     CDS             complement(<1..900)
                     /locus_tag="ACHOTW_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFP9846677.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSILVFSFN
                     GGIISKIISNKVMILLGDASFSFY"
     gene            complement(910..1590)
                     /locus_tag="ACHOTW_004730"
     CDS             complement(910..1590)
                     /locus_tag="ACHOTW_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFP9846678.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1577..2044)
                     /locus_tag="ACHOTW_004731"
     CDS             complement(1577..2044)
                     /locus_tag="ACHOTW_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFP9846679.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(2044..2580)
                     /locus_tag="ACHOTW_004732"
                     /pseudo
     CDS             complement(2044..2580)
                     /locus_tag="ACHOTW_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2644..3291
                     /locus_tag="ACHOTW_004733"
     CDS             2644..3291
                     /locus_tag="ACHOTW_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9846680.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT          981 a          848 c          827 g          890 t
ORIGIN      
        1 gtaaaatgaa aaagaagcat cacctaaaag tatcataact ttatttgata ttatttttga
       61 aataatacca ccattaaacg aaaacactaa tattgaaaag gccattgatg gtataaaaag
      121 cgcatcatac tgtaatccat tgctattttg actttttgcc accatataga cagacattag
      181 gataaagact acagatgata tttcagcaaa tgatagaact aaatatgacg gctttgactt
      241 aaactcaaaa tctccggcct gattgctgcg gattgtctct gccagcctgc ctcggtcaat
      301 ctcacgacca gccatcttgt cctgaacatt gaagtagtca atcggaccga gagcagccat
      361 cccaaggtga taaacaaact caccaaaccc tgaaggattc tgctgataca tctgagcaac
      421 gttgttaggg tcaacaccga cgcgcgccag ttccttggcg ttgttttgca gccatgattg
      481 cattgcttct ggagacgagg ccgcaaggcg tgcgccagcc gctaaggtgc cgatagaatt
      541 gcgctggtct tcgtctgccc acttcatacc agactgaatc ttctctaatt gaccaggata
      601 tttggtcatc agatctcgaa cctgctgtcg atcgccggac tggatggctg ccgcatattc
      661 tttttggaat gcagcatccg cttcctgttg ctttgcggct tgagatgttt gagcgacact
      721 accaagtccc tgcaacgcct gaaggccgat gttattgcga cctgaacgct ccatttcgtt
      781 gttctggcga atataggcca acgcctcact tacatcactt gcctttggcg catttgagtt
      841 ttgcccaccg ataccagcaa gaaaaccgcc tgagttgatt ccttgttgcc aagtagccat
      901 attcccacct taaaacaatg atccaagacc accgataaca ccgccagcaa gagcaccaat
      961 accagtccca ataacaggaa ctgcactacc aatcattgca cctgatgcgg ccccaccaat
     1021 ggcacctgta gcaaaactct gtaggcctga aggcttattc gcatttgctg ccgatgctgc
     1081 cgcctgctgt tgatacaatt ggctgacatt gttagcgtag ttctgtccgg cgtttgcctg
     1141 acctgtaaga gcaccaaggc cgatatttgc cagattgttg tagttgttca tctgacctga
     1201 cagccagttt tgaccgagtg taggtgcgat tgctgctaac tggtttcctg ttgctgtaga
     1261 gcctaatcca cccgttgcct ctgctgctgc cagactctga tagcgcgcct gccctgcaag
     1321 gtctttatac tgctgagagt tgtaatactg gttaagcgcc tgaccttgcc cctgaagaga
     1381 ggaaagattc tgcaactgtg atacgtactg ctgagcgagt ggcgtgaacg gtgcaaggtt
     1441 ttgcatgttc gtctgccaca tttcacgctg cagttcgatg cccttttcag ttgcgcgtgc
     1501 ctgggcttta gatccggagt cgctgccgcc tttcatatac ccattcatgg gaagcaattt
     1561 attcttgaag ctttcgctaa gtactaacat ttaatagctc ctcatatttc gaacgaggta
     1621 attgatagag ggtgattcct accggttttc cgttactcat gtacgcatca tcaaggtgac
     1681 caacacgggt agcgccaagc aaacggataa ttgcccgtcc gtatttggtg gtgtcaggaa
     1741 ccatggtgat gctgttaagg aatggtgagt tttcgagaag ccatttgcag aataatcgat
     1801 gcccttgtag tgcatattcg ccacggaatc cggggtcata caccgcatgg cattcaacaa
     1861 cgctatgcca gaagttacgc acttcatgaa ctccagccag cactaatcct tcgtagatgc
     1921 cgagatatac cgcatcaggc ttgatgtagt atttatctcc actgtctacg atattccccg
     1981 tgtttaccgg gttgttaagg aattctgcaa gtttcaccgg gttatcgatg agctttattt
     2041 ccatcactgc tccgcaatga ttttgatggt tgtggcagta aacgccgcgc cattcgactg
     2101 aatggttaac gtgctgccat ttgtggcaag aaagccgtct ttatccacgc tgaagaacgt
     2161 agctaacagg atgttgtcgg ttgttgttgc cgagttgcga ctgcttacta gcgtgtcagg
     2221 aacagaaccg gagaatgtta gctgcattga cctgttggcg gttccgctgg gccacgtccc
     2281 gacaatcgac agcttgaaga acaaggtttt gttctcgttg aacacaacca tcttgttgtt
     2341 aacggtgtcg aagaatggtg ccaacgtccc ggatgacggc gtaagcgttt tcagcaggct
     2401 aacaaggttg gtcggcgctg tcgggatggt tactgatacg ccagagtaaa caacctctga
     2461 cttcttgcgc gtagtggcat actccagagc attaatgcgc gtttcatggt ctgaaacctg
     2521 cgattccagc gactgaactc tggtatcaag cgatgcaata tcgctttcat tcaaggcaat
     2581 cagtgggttt acgtcatgga gtgggctcaa caccagtcag agccgcatgt acacgcaata
     2641 atcatggtga acgaaagaag cattaatggg caagctggca aaattgtcag caaatgggta
     2701 acactggcaa acaatgcaat cccatcaaat gttcctcccc agtgggatct agatttgaaa
     2761 ggtcagcaat attcagagcc tacaaagtgt ataggtaaac agattgatta catggcaaag
     2821 cccgaaacta agttaaatga ctttaataga gaggcgagca agagcctata tgattggtca
     2881 gatgccaacg tttggggtta ttcgaatggc tgggcgcggc atgagataaa aaaagaaaca
     2941 ctttctatca ctggctttca tgctgtaaga cgcatactaa aagccgttca ggcatcaagg
     3001 aaagacaact acaaagctaa gcgacagata cagcgtacat tgaaacgaca agacataaat
     3061 cgatcaccaa tcaccccgtt ccaagcaccg ttaattagca aacacgattg ggatttatca
     3121 gtgatagcgg tcaccaaaga ggcagagaca gagcgcaggg aattactatc ctcacagata
     3181 ataaactcga acaaagtaca aacccaccca ctgttaagga tgttaagaag agcacgagtg
     3241 agagcatcca gagccaaaag agaaagcgga ggttatgacc attactacta gtagtgacag
     3301 atcgtgacaa ggcggtgcaa aaaacgacca ataataagag agatatgcct gccaaaccgc
     3361 caaaagcgcg gagcgcggca aacgccgcga gaggcgcaag aaggcgaacc tatccattga
     3421 aacggtcaga agcgcatcgc gctcgacgac cgaagtcccc actaacaccg ttaacgaaag
     3481 atcaaagtct tagaaaaaaa tgacgtttct ttgatggact tccatccgta aaaatcaaat
     3541 tttgag
//
LOCUS       DAZKIF010000251         3542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZKIF010000251 DAZKIF010000000
VERSION     DAZKIF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.253"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..825
                     /locus_tag="ACHOTW_004734"
     CDS             <1..825
                     /locus_tag="ACHOTW_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9846681.1"
                     /translation="SDWVEVGPFGNGEKLKVKIESLGKTSDTIGKSSNAQAVLPYVVK
                     IARGTPDFTGERKSTWFISDTVIANIGGESSSSIDFWLGICKALKFNWCVNYLTSKLA
                     GDTFTLGLNISYYPKNTTCKPENTVIKVDDIALFQLRNQGKIAANSKEGTITLKCDNL
                     FGDKKQASRNMVVYLSSSDLVKGSNTILRGKTDNGVGFVLDLTEPPKGTEAAIKISAN
                     GDQGAATSLWKTDKPGVSLNSNIINIPVMASYYVYDEKKVKSGALEATALINVKYD"
     gene            complement(907..1245)
                     /gene="rcnB"
                     /locus_tag="ACHOTW_004735"
     CDS             complement(907..1245)
                     /gene="rcnB"
                     /locus_tag="ACHOTW_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP9846682.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1463..2314)
                     /gene="rcnA"
                     /locus_tag="ACHOTW_004736"
     CDS             complement(1463..2314)
                     /gene="rcnA"
                     /locus_tag="ACHOTW_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP9846683.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHEHHHHHEHGDNEEYQD
                     AHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATL
                     VVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVH
                     GFMGIMR"
     gene            2435..2707
                     /gene="rcnR"
                     /locus_tag="ACHOTW_004737"
     CDS             2435..2707
                     /gene="rcnR"
                     /locus_tag="ACHOTW_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP9846684.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(2817..3131)
                     /locus_tag="ACHOTW_004738"
     CDS             complement(2817..3131)
                     /locus_tag="ACHOTW_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFP9846685.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(3141..3488)
                     /locus_tag="ACHOTW_004739"
     CDS             complement(3141..3488)
                     /locus_tag="ACHOTW_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846686.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
BASE COUNT         1001 a          735 c          828 g          978 t
ORIGIN      
        1 agtgattggg ttgaagttgg gccttttggt aatggcgaaa aattaaaggt taaaatagag
       61 tctttaggta aaaccagcga cacaattggg aaatccagca atgcgcaggc agtattacct
      121 tatgtggtta aaatagccag aggcacacct gattttactg gagaaagaaa atctacctgg
      181 tttatttcag ataccgtgat tgcaaatatt ggcggtgagt catcgtcatc catcgatttt
      241 tggttgggta tttgtaaggc attgaagttt aactggtgtg tgaattatct caccagcaaa
      301 ctggcggggg atacatttac gcttgggtta aatatttcct attatcctaa aaatacgacc
      361 tgtaagcctg aaaacaccgt tataaaagta gatgatatcg ccttgttcca gctcagaaat
      421 cagggaaaga ttgcggcgaa cagtaaggaa ggaacaatta cgttgaaatg tgataatctt
      481 ttcggcgaca aaaaacaagc atcgcggaat atggttgtat atctttctag cagtgactta
      541 gttaaaggaa gtaatactat tttgcgtggt aaaacagata atggtgtagg gtttgtgttg
      601 gatctaacag aaccaccaaa agggactgag gctgccatta aaatttcggc caacggcgat
      661 cagggcgcgg cgacatcatt atggaaaaca gataaaccag gagtttcatt aaatagcaac
      721 attattaata taccagtcat ggccagttac tatgtatatg atgaaaaaaa agttaaatct
      781 ggcgcactgg aagcaaccgc attaatcaac gtgaaatacg attaataaca agcaagatga
      841 gcaagtggct aataataaga aataataagg tgtctgcatt tccccctctc tccgaggggg
      901 ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt taccgtcggt
      961 gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg catccggcgc
     1021 gggcaggtta cgcaactgcc actgcttaat gttgtactca tcggtacggt acatctctgg
     1081 tacggtgtcc cctggtttgt aatgcttaaa gtcagcaatg aaactgctta actcatactt
     1141 agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg cggcactggt
     1201 aaccagcaaa agcgcaccca gcaacatctt atttttaatg gtcataaact ctccactttg
     1261 gggatctgta atatcagtcg catatctttc ctgccataca atataactga caagcgatat
     1321 tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgtag caaaagcatc
     1381 ctcattgagc gagaagtgag cggaataagc tgatttaaga atcatcctgg gctaaaagga
     1441 tagcgaagcc caggtgattg ttttatctca taatacccat aaagccatgc actcccatat
     1501 acaccccgac taaaccaatt aacagactgg aaaaataggg ggcgcgttta gcgagagtgt
     1561 taaatccgct ccagcgtttt gcaacttgtt gaacactgat tgccgctcca acgcctactg
     1621 taaccagcgt tagtgccaga ccgatgctga aactgacgac cagtgttgcg cccagtgtca
     1681 gggctttcaa ctgaatgcaa atcaacagca cggtaattgc tgccgggcag ggaataaggc
     1741 cacctgttaa gccaaataac aaaatttgcc agttggtgac ctctctacca tcaaagcgtc
     1801 gtttaatgtc attggcatgg gctcgtgcat gggcatcctg atactcttcg ttgtcgccat
     1861 gttcatgatg gtgatgatgt tcgtggtcgt ggtcgtggtc gtggtcgtgg tcgtggtctt
     1921 gatgatgttc gtgatcgtgg tgatgatgct catgcatatt ctccagccag ttgcgttcgc
     1981 cgcgccaggt acgccagaac atccagaaag cggtgctaat aatgatcact gcggaaatca
     2041 actggagcca cggttctgct gattgagcag taaagcgctt gctgatcacc atcccgccaa
     2101 aggcaatcaa ccagaccact gcggtatgcg aaatggttgc tgccagtccg agcatcaccg
     2161 cctgtttaat ggtgcctttg atggcgatga taaacgccgc catcatcgtt tttgagtgcc
     2221 ctggttccag gccatgaagc gcaccaagta agatggcgct ggggatgaag aaccaggcgt
     2281 ttccttgctg aagaagagtt gtaaattcgg tcatgataat aattcttagt attaattcgg
     2341 caatctgatt ctactccccc ccagtacctg atactacccc ccagtagatt aatagtgcta
     2401 tgatttttca tgttcttgtt aaccaggtgt tgccatgtct catacaatcc gtgataaaca
     2461 gaaactgaaa gcgcgtgcca gtaagattca gggccaggtc gtggcgctca agaaaatgct
     2521 cgacgagccg cacgaatgcg ctgcagtttt acaacagatt gctgctatcc gtggcgcggt
     2581 aaacggtctg atgcgggaag tgattaaagg tcatctaacg gaacatatcg ttcaccaggg
     2641 ggatgagcta aaacgtgaag aagatctgga tgttgttctg aaggtgctgg attcttatat
     2701 caaatgaaag tgcctaatat ggccgtagtt gaggcaataa atagagggac aagtgacgtt
     2761 aagctgaatt agagtaaatg gcgtgaggac acacgccatt cgaggttata gtttgattaa
     2821 ttttcgaaaa attgcttcac agcccagcca ccatgaccac ctggagttat gaatgaaaca
     2881 aaacctttgt catctacgct ctggatttct accgtcattg attcatctaa cacgcgacat
     2941 ttattggatg taatgagact cagcatagcc tgttggtcat tgtcctggat gtaactgatc
     3001 atctcgtaaa agtcagttaa ctttttacag ttaaatgtac cttctttaag tgttgcgctg
     3061 tctccgtgac ttagtgcgaa tgcatttggc atcattgcca caaagatgac ggtaacgagt
     3121 agttttttca ttttagcctc ttataattca gaacattcac tgatgttgta agtgcctttt
     3181 ctaccatcat cgtttacgag atgtagcacg aagatgatgg gattggcatc aatgaaagat
     3241 acagagacgt tcccgctttc ggtgctatac atctttgcaa tctgtttttc accaatatat
     3301 gagagatccc aactcactcc gttcaagcta aaatgcatct gtttgccgtc aatatcgatg
     3361 gaggattgtg ccgtaacggt tttcccattg ctgccaattt gaccctgctg aagagcatca
     3421 catttatagg agcttgcata agtagataga ggtagtagca gaagtgcagc aaaaatttta
     3481 ctcttcatat tttccctttg cttggatttg ggttacctat tttggggtag tcattataaa
     3541 ac
//
LOCUS       DAZKIF010000252         3513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZKIF010000252 DAZKIF010000000
VERSION     DAZKIF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.254"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..224)
                     /gene="escO"
                     /locus_tag="ACHOTW_004740"
     CDS             complement(<1..224)
                     /gene="escO"
                     /locus_tag="ACHOTW_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFP9846687.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRS"
     gene            complement(284..1624)
                     /gene="escN"
                     /locus_tag="ACHOTW_004741"
     CDS             complement(284..1624)
                     /gene="escN"
                     /locus_tag="ACHOTW_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFP9846688.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1608..>3513)
                     /gene="escV"
                     /locus_tag="ACHOTW_004742"
     CDS             complement(1608..>3513)
                     /gene="escV"
                     /locus_tag="ACHOTW_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFP9846689.1"
                     /translation="IIAINISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSV
                     STTRLILLHHDAGDIIYSFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSA
                     RFSLDGMPGKQMSIDGDMRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIA
                     GIIIVLVNLFGGVLIGMWQFDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVT
                     RVPGESEKEENLAGDIVQQVSVNSRPFLISAALMLVMAIIPGFPALVFLFLAVCLLGI
                     AWKLQKKRTFGTGNNKDAMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRW
                     NFFEESGIPLPKIIVNPVKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFV
                     QEKLSTNSIVYKTNKTNQQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETR
                     YLMDIMERKYNELVKELQRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEK
                     DVVILCEYVRIALRRHILGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISP
                     ERTEQIIGFLKNIMNPTGNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELK
                     VLGTVNDFRA"
BASE COUNT         1061 a          831 c          646 g          975 t
ORIGIN      
        1 aatgaacgct tttccttcga aagtgaagat tttttctcat acaatcgctg cttttgctca
       61 tcaaatgcat tgtttttaat ctgatattta tacaacgaca ctttatccaa attcaccagc
      121 gatgcacaat atgcctgctt agaggcaatc gactctttaa tggattgctc gcagtcatcg
      181 agtttaccat ccacctcttt tatctgagag ttaatcttag ccattgattc tctcaatcgg
      241 ttcgctctgc ttttacgaat agataaaatt ctgtccaaca tactcaggca accactttga
      301 ataggctttc aatcgttttt tcgtaactac tgatatcttt ggtgctttgt tgtataaagt
      361 tctgaattaa tttcctattt tttattgcct tatctgcttc aggatcttgc cccatcgtgt
      421 actcaccaat acgaataagc agctcaacat ttttataagt tgcaataagc tttttgcatt
      481 ctgccgccgc acgcaaatgc tccgatgtaa caacgttatg cataacacga ctggcactta
      541 aaccaatatc aattgcaggg aaatggtttt cctctgcaag ttctcgggta agtacgatgt
      601 gcccatcaag aatagagcgg acttcatcgc caataggatc attaacatta tcgctttcta
      661 acaacaccgt ataaatcgcg gttattgaac cttttggcgc aggccccgct cgctctaata
      721 atttgggtaa cgaagaaaaa acactcggag ggaatccccc ccttacatca ggttctcccg
      781 atgcaagccc aacatcacgt gcggctcggg cataacgagt tacagaatcc atcattaata
      841 atacattttt accttgatcg cggaagtact ctgcaatagt cgtcgccgta aatgcggctt
      901 tcatcctttc cagcgcgggg cggtctgacg ttgtgacgac cagtacacat ttagaaagcg
      961 tggattgagg taagagcgcg aggaattcat ttacttcgcg accacgttcg ccgataagag
     1021 caaggacaat aatatctgcg gatgcgccat tacaaatcat ccccaaaagc gtacttttac
     1081 caacgcctga accagcaaaa ataccgatac gctgcccaat accgcaagtt agtagcccat
     1141 caattgccct gacgccaagt ataaatggct gatcaataac ctgccttaat aaaggatctg
     1201 gtggttcagc atacaagctg cgttcaaacg gaagataggg ggcagcaata ttactctcca
     1261 ttggtctgcc tatgccatca acaagccgcc caagtagtgc gtctccaaca cgaatcttga
     1321 actcatctcc ctgataactt aaccactgac cacagtacat gccggaaaca tgctcaaaag
     1381 gcaacaagaa aacctcatct tcatcaatag cgatcacctc ggctaaacgt tgactgggtt
     1441 ctatcttata aaaggcgcca atacgcgctt ttggtaaacg cgccttaata atcgtcccgc
     1501 cgatattgat aatcttgcct tcatatctgg tagacgaatt taatgataat gccggcacag
     1561 tgctattgag cactttctga atacgtgggt atttttccaa tacagaatca tgctctgaaa
     1621 tcatttaccg ttcctaatac tttaagttcg atattattcc caacctcctg aaaagagagc
     1681 acggggactg acgggaacga accttcaatc attttcttca catagcgcct gatatctaaa
     1741 gcggtcagaa tgacgccgtt ccccgttgga ttcatgatat tttttaagaa gccaattatc
     1801 tgctcagttc gctccggtga gatattcaga tacgaacctg atgacgtttg tctgatcgac
     1861 tctcgtagct cattttcaat atcagagcca ataagccaaa cgttcagaag tgtaccgcta
     1921 acgctatagc gacctaaaat atgccgacgc agggcaatac gaacgtattc gcacaagata
     1981 accacatcct tttcttttgt tgaccaaaaa ataagcgtct caaagatagt tctcaggtct
     2041 ctaattgaga cattttcctc tacgagacgt tgtaggatgt caacaatttt gctcaaacca
     2101 agctggcgct gcagctcttt cacaagctcg ttatattttc tctccatgat gtccatcaaa
     2161 taacgcgttt cttgtacgcc gatgaactct ttggcattag acaatacaag tttctttagc
     2221 aaaaacgtta tcttatcatt tgttgttgca taaacatcca tacctgtaag gtgagcgagc
     2281 tgttgattag tcttattcgt cttatatacg atagagttag tcgaaagctt ttcctggaca
     2341 aattcgaatg atatctctgc atgcccagcc tcaaaataga cagtatcatc tataagagta
     2401 tctttgtata ttgactcttg atagagcaaa aattctattg cgctatcatt atttttaacc
     2461 ggattaacaa taatcttagg caatggaatt ccactttcct caaagaaatt ccaacgcatg
     2521 acctcaattt gctgtgtaat gtctgaacta taaatattac tacttaagtt taaaatcaat
     2581 ggctcagcgc ccggtgagat gttttggcta ttagacaaat cagctcccat agcatcctta
     2641 ttattgccag ttccaaatgt tcttttcttc tgtaatttcc aggctatccc caacaggcaa
     2701 accgccagaa ataagaagac caatgcagga aagcccggaa taatcgccat tacgagcatt
     2761 agtgcggcac tgattaaaaa agggcgacta ttaacagaaa cctgttgaac aatatcacct
     2821 gcaaggtttt cttctttttc gctttcaccg ggcacacgag taacaaccac gcctgcggtg
     2881 acagaaataa taagcgcagg gatctgggca accaaagcat caccgacaga caatacagaa
     2941 aacaggctaa gtgccgcact aaatggcatg tcaaattgcc acataccaat gagcacgcca
     3001 ccaaaaaggt taactaaaac aataatgata ccagcaatag cgtccccttt tacaaacttc
     3061 atcgccccgt ccattgaacc ataaaactgg ctttcctttt gcactcggga gcgaagaact
     3121 tttgcctcaa gcgggtcaat aacgcctgcg cgcatatcac catcaatact catttgcttt
     3181 cctggcatac catcaaggga aaaacgagca ctcacttcag caacacgctc ggcccctttt
     3241 gtaataacca taaattgaac aatggtgata atagtaaaaa taaccaggcc aacaacaata
     3301 ttaccgccaa caacaaagtt accgaaggag taaataatgt cacccgcatc gtgatgcagc
     3361 agaataagtc tggttgtact aacactgagc gacaggcgca tcaacgtcgt aatcaacagg
     3421 attgtgggga aagaagttag ttcaagagga ttttttatat aaattgagag cattaataaa
     3481 agtaaagctg tcgaaatatt aatcgcgata atg
//
LOCUS       DAZKIF010000253         3417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZKIF010000253 DAZKIF010000000
VERSION     DAZKIF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.255"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            142..1107
                     /gene="dusB"
                     /locus_tag="ACHOTW_004743"
     CDS             142..1107
                     /gene="dusB"
                     /locus_tag="ACHOTW_004743"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9846690.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1133..1429
                     /gene="fis"
                     /locus_tag="ACHOTW_004744"
     CDS             1133..1429
                     /gene="fis"
                     /locus_tag="ACHOTW_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP9846691.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1514..2398
                     /gene="yhdJ"
                     /locus_tag="ACHOTW_004745"
     CDS             1514..2398
                     /gene="yhdJ"
                     /locus_tag="ACHOTW_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9846692.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            2482..2661
                     /locus_tag="ACHOTW_004746"
     CDS             2482..2661
                     /locus_tag="ACHOTW_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP9846693.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2664..3326)
                     /gene="acrS"
                     /locus_tag="ACHOTW_004747"
     CDS             complement(2664..3326)
                     /gene="acrS"
                     /locus_tag="ACHOTW_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP9846694.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          935 a          791 c          810 g          881 t
ORIGIN      
        1 agtaattatc gcaccacgct cattttaaat gcaattcttt gatccatctc agaggattgg
       61 tcaaagtttg gcctttcatc tcgtgcaaaa aatgcgtaat atacgccgcc ttgcagtcac
      121 agtatggtca tttcttaact catgcgcatc ggacaatatc agctcagaaa tcgcctgatc
      181 gcagcgccca tggctggcat tacagacaga ccttttcgga cgttgtgcta cgagatggga
      241 gccggattga cagtatccga gatgatgtct tctaacccac aggtttggga aagcgacaaa
      301 tctcgtttac ggatggtgca cattgatgaa cccggtattc gcaccgtgca aattgctggt
      361 agcgatccga aagaaatggc agatgcagca cgtattaacg tggaaagcgg tgcccagatt
      421 attgatatca atatgggttg cccggctaaa aaagtgaatc gcaagctcgc aggttcagcc
      481 ctcttgcagt acccggatgt cgttaaatcg atccttaccg aggtcgtcaa tgcagtggac
      541 gttcctgtta ccctgaagat tcgcaccggc tgggcaccgg aacaccgtaa ctgcgaagag
      601 attgcccaac tggctgaaga ctgtggcatt caggctctga ccattcatgg ccgtacacgc
      661 gcctgtttgt tcaatggaga agctgagtac gacagtattc gggcagttaa gcagaaagtt
      721 tccattccgg ttatcgcgaa tggcgacatt actgacccgc ttaaagccag agctgtgctc
      781 gactatacag gggcggatgc cctgatgata ggccgcgcag ctcagggaag accctggatc
      841 tttcgggaaa tccagcatta tctggacact ggggagttgc tgcccccgct gcctttggca
      901 gaggttaagc gcttgctttg cgcgcacgtt cgggaactgc atgactttta tggtccggca
      961 aaagggtacc gaattgcacg taaacacgtt tcctggtatc tccaggaaca cgctccaaat
     1021 gaccagtttc ggcgcacatt caacgccatt gaggatgcca gcgaacagct ggaggcgttg
     1081 gaggcatact tcgaaaattt tgcgtaaaca gaaataaaga gctgacagaa ctatgttcga
     1141 acaacgcgta aattctgacg tactgaccgt ttctaccgtt aactctcagg atcaggtaac
     1201 ccaaaaaccc ctgcgtgact cggttaaaca ggcactgaag aactattttg ctcaactgaa
     1261 tggtcaggat gtgaatgacc tctatgagct ggtactggct gaagtagaac agcccctgtt
     1321 ggacatggtg atgcaataca cccgtggtaa ccagacccgt gctgcgctga tgatgggcat
     1381 caaccgtggt acgctgcgta aaaaattgaa aaaatacggc atgaactaat tcaggttagc
     1441 taattgcttg attaaaaggc gctactcggc atggggaagc gcctttttta taggtgtcac
     1501 aaagggagtg accatgagaa caggatgtga accgacccgg tttggtaatg aagctaagac
     1561 cattattcac ggtgatgccc ttgccgaact taaaaagcta cctactgaaa gcgtcgatct
     1621 gatctttgcc gacccaccgt ataacatcgg taaaaatttt gatggtctga tcgaagcctg
     1681 gaaagaagat ctgtttatcg actggctgtt agaagtgatt gcagagtgcc accgcgttct
     1741 gaaaaagcag ggcagcatgt acatcatgaa cagtacggaa aacatgccct ttatcgatct
     1801 ccagtgccgc aagcttttta ccatcaaaag tcgcatcgtc tggtcatatg acagttctgg
     1861 agtgcaggcg aaaaaacact acggctccat gtacgaaccc atcctgatga tggtgaaaga
     1921 cgcaaagaac tacacattca acggtgatgc tattctggta gaagccaaaa ccggatcgca
     1981 gcgcgcgttg atcgattatc gcaaaaatcc tccacagcct tacaatcatc aaaaagtacc
     2041 gggtaacgtc tgggattttc cgcgcgtgcg ttatttgatg gatgaatatg aaaaccaccc
     2101 gacgcaaaaa ccggaagcct tactgaaacg cattattctc gcctcttcca acccaggcga
     2161 tatcgttctc gacccgtttg ctggtagctt tactaccggt gccgtagcca tcgccagcgg
     2221 acgaaaattc attggtattg agatcaacag cgagtacatc aaaatggggc ttcgacggct
     2281 ggatgtcgcg tcgcattact ctgcggaaga actggcgaaa gtgaaaaaaa gaaagaccgg
     2341 caacctgtca aaacgaagcc ggttaagcga agttgacccc gatctcatta caaagtaaag
     2401 gatgtgtaag cctggtttgc agattattca tttcgtgtat atttcctgcc agacttggtt
     2461 aaacatgcac aggctcaggt aatgattcgc aagtattggt ggctcgtcgt tttcgctgtc
     2521 ttcgtttttc tgtttgatac tttactgatg cagtggattg aactgctggc aacagaaaca
     2581 gacaaatgcc gcaatatgaa ctcagttaat ccactaaaac tggttaactg tgacgaactg
     2641 aattttcagg acagaatgtg aatttacatg acacttaatt cattcgtttg atgaattaat
     2701 ttcgttatgt tttcatctgg catgaacatt cttaatacgt tatcgaccag agcgggggct
     2761 tgtttataaa gatcataacc cgccatattc attaaccagt tttgaacaat tccgctgaag
     2821 gcaccatcaa taataatcat cacaacatct aaatcgaggt tatttgctac acaaccttgt
     2881 tgctgacacg cctgcaatac ttcgcggaga gtctgcggat taaagcccat cttttcgcgt
     2941 atcactccct cggccagcat ctcatcatta aattcacatt tgtgatataa gattttcagc
     3001 aacgcctgct ggcggggaat tttggcaata tattgcaagc cgacaatcaa tttttcacgc
     3061 aattgttgaa acgggtcatg ctctaatcca gccgtcaagt gttcctggat taactcccgc
     3121 aatgaaggct gttgcaacca catctcatta aacagttggg tcttgttctc gaagtgccag
     3181 tagatagcgc cacgcgtaac gttagcggca tcggcaatgt cgttgagcgt cgtcttgctt
     3241 acgccatgtt gcgcaaactg ggcgatggca gtttcaatca gttcttgccg ggtcttcaga
     3301 gcttcggctt tggttctttt tgccatgatt aattattcag gaaataaata tcttcgacac
     3361 agagtgagaa aatagcgaag gttaatctat cacctaatgt gtatttatac gagaggc
//
LOCUS       DAZKIF010000254         3415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZKIF010000254 DAZKIF010000000
VERSION     DAZKIF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.256"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..577
                     /locus_tag="ACHOTW_004748"
     CDS             <1..577
                     /locus_tag="ACHOTW_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846695.1"
                     /translation="FSLTSDGKLTAKNADISGSVNANSGTLNNVTVNENCTIKGMLEA
                     TQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAY
                     SDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEF
                     KVFHKGNQRAGNITDCTVIVTKKAASGISIR"
     gene            648..1247
                     /locus_tag="ACHOTW_004749"
     CDS             648..1247
                     /locus_tag="ACHOTW_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9846696.1"
                     /translation="MRKVCAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1307..2623
                     /locus_tag="ACHOTW_004750"
     CDS             1307..2623
                     /locus_tag="ACHOTW_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9846697.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTISVYEDSQPGTLNDFLGAMTEDDARPE
                     ALRRFEQMVEEAARHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASESA
                     RQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNAARDATT
                     STEKARESAESAQSAEQSRIAAEDAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERG
                     DTGPAGATGERGPGGDTGPAGPQGPKGDRGERGETGLTGSTGPQGPKGDTGATGPAGP
                     QGPKGETGAAGPVGATGPQGAKGDPGETQIRFRLGPMRIIETNSYGWFPGTDGALITG
                     LTFLDPKDATQVQGMFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            2625..2894
                     /locus_tag="ACHOTW_004751"
     CDS             2625..2894
                     /locus_tag="ACHOTW_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9846698.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGRSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            2896..3003
                     /locus_tag="ACHOTW_004752"
                     /pseudo
     CDS             2896..3003
                     /locus_tag="ACHOTW_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3071..>3415
                     /gene="nleB2"
                     /locus_tag="ACHOTW_004753"
     CDS             3071..>3415
                     /gene="nleB2"
                     /locus_tag="ACHOTW_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFP9846699.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIY"
BASE COUNT          944 a          726 c         1027 g          718 t
ORIGIN      
        1 attttccctg acatcagacg gaaagctgac cgctaaaaat gcggatatca gtggcagtgt
       61 gaatgcgaac tccgggacgc tcaacaacgt cacggtaaat gaaaactgta cgattaaggg
      121 catgctggag gcgactcagg tcagaggtga cttcgttaaa gctgtatcca aatcatttcc
      181 gaaacaggct ggtacgtggg gtaacacgga aacaccaaac gggacggtta cagtcaccat
      241 cagcgatgat cataactttg accgtcaaat cattattccg cccattatct ttaacggaat
      301 agcgtatagc gatccgggaa gtggtaataa cccgggaggt acaagataca cgggttatgg
      361 ttttgaagtt cgcaaaaacg gtgtattaat cgcatccaga gaaactaaag gggccattcc
      421 cggtagctac agtgcggtta ttgatatgcc gagtggcagg ggaagcgtca ctctggagtt
      481 taaggttttc cataaaggca atcagcgggc aggtaatatc accgactgta cggtgattgt
      541 gaccaaaaaa gcggcttccg gcatcagtat ccgttgaaat atttataacc ctaataacgg
      601 gcgccagaaa tggcgccttt tttattgcag aaaatcggga ggtaattatg cgtaaagttt
      661 gtgcagcaat tttgtccgca gccatttgtc tggccgtatc cggtgtgcct gcatgggcgt
      721 ctgagcatca gtccacgctg agcgcaggat atcttcatgc cagtacggac gctcccggca
      781 gcgatgatct gaacgggatt aacgtgaaat accgttatga gtttacggac acactggggc
      841 tgattacgtc cttcagttat gccaacgccg aagatgagca aaaaacgcat tacagcgata
      901 cccgctggca tgaggattac gtgcgtaacc gctggttcag cgtgatggca gggccgtctg
      961 tgcgtgtgaa tgaatggttc agtgcgtatg cgatggcggg tgtggcttac agccgtgtgt
     1021 cgactttttc cggggattat ttccgcgtaa ctgacaacaa gaggaaaacg cacgacgtgc
     1081 tgaccggaag tgatgacgct cgctacagca acacatctct ggcgtggggg gctggcgtgc
     1141 agtttaaccc gaccgaatca gtggccgttg atgtcgctta tgaaggctcc ggcagcggtg
     1201 actggcgcac tgacggtttc atcgtgggtg tcggttataa attttgatta gtaacacagc
     1261 gttatgacag cccgccggtt caggcgggct tttttgtgga gtgggtatgg cagcagtaca
     1321 aatatcaggc gtgctgaaag atggtgcggg aaaaccaata cagaactgca ccattcaact
     1381 gaaagccaga cgtaacagca ccacggtggt ggtgaacacg gtggcctctg aaaatccgga
     1441 tgaggcaggg cgttacagca tggacgtcga gtatggtcag tacagcgtca ctctgttggt
     1501 ggagggattc ccgccatcac atgccgggac catctccgtg tatgaagatt ctcaaccggg
     1561 tacgctgaat gattttctcg gtgccatgac ggaggatgat gcccgtccgg aggcactgcg
     1621 tcgttttgaa cagatggtgg aagaagcagc gcgtcacgca gaggaggcga agaagaatgc
     1681 cggagaagca gagacgtccg cgaggaatgc cggcatatca gccagtaagg cggaagcgag
     1741 cgccgcaaat gctgatactt cagcagagga tgcatcggag tcagcccggc aggcggcaga
     1801 aagtgcagcc tctgcaaaga agtcagagga agcgtcctcg tcctcagcct ctgaggccgc
     1861 tcaaaaagcc agtgagtcat tacaaagtgc aacagatgcc gagttgtcaa aaaagacggc
     1921 agaaagtgca gccggtaatg cagccaggga tgcaacgacc tcaacagaaa aagcccggga
     1981 atcagcagaa agcgcacagt cagcggaaca aagcagaata gcggcggaag acgccgtaaa
     2041 cagaattccc accgtggtgg ggcctcccgg accaaagggg gaaccgggtc ccgcgggtcc
     2101 tcaggggccg aagggagata aaggagagcg tggagacacc ggtccggcag gggcaaccgg
     2161 tgaacgggga ccgggaggag atacaggtcc ggcaggtccg caggggccga aaggcgacag
     2221 gggagagcgg ggagagaccg gtctgacagg aagtacaggt ccacaggggc caaagggaga
     2281 taccggggca acaggtccgg caggaccgca gggaccgaaa ggggaaacag gtgcggctgg
     2341 cccggtgggg gctaccggac ctcagggggc gaagggcgac ccgggggaga cacaaatacg
     2401 gttccgtctg gggccgatga gaattattga gacaaacagc tatggctggt tcccgggtac
     2461 agatggtgcg ctcatcaccg gactgacctt tcttgacccc aaagatgcca cacaggttca
     2521 ggggatgttt cagcatttgc aggtcagatt tggtgacggg ccatggcagg atgttaaggg
     2581 actggatgaa gtgggcagtg atacaggcag aactggagaa tgacatgaat attttgagaa
     2641 agcttatgca gagtctgtgc ggttgcggaa agcatgatga ctgtgaaaac ggacggtcac
     2701 ttacagcaca attgcgactg ggaccggcag acattctgga gtcagatgag aatggcatta
     2761 ttccggagca ggacagggta atcacacagg tggtgatact ggatgcagat aaaaagcaga
     2821 tacagtgtgt ggtaagaccg ctgcaaatcc tgcgtgctga cgggacgtgg gaaaatattg
     2881 gcgggatgaa gtaacccgac agcttcacaa aaccggagtc cggctccggt ttttgttgtc
     2941 atgtccggtg gatgtttgtt aggaaagcaa agatggcaaa actgctggag gttttgtggt
     3001 tgagtatgcc aatataatta atagattaaa gagttagttg tgaagaaaat atggataaac
     3061 aggacgacga atgctttcac cgataaggac aactttccat aactcagtaa atatagtgca
     3121 gagttcaccc tgtcaaacgg tttcttttgc aggaaaggaa tatgagttaa aggtcattga
     3181 tgaaaaaacg cctattcttt ttcagtggtt tgaacctaat cctgaacgat ataagaaaga
     3241 tgaggttcca atagttaata ctaagcagca tccctattta gataatgtca caaatgcggc
     3301 aaggatagag agtgatcgta tgataggtat ttttgttgat ggcgattttt cagtcaacca
     3361 aaagactgct ttttcaaaat tggaacgaga ttttgaaaat gtaatgataa tctat
//
LOCUS       DAZKIF010000255         3398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZKIF010000255 DAZKIF010000000
VERSION     DAZKIF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.257"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(417..584)
                     /locus_tag="ACHOTW_004754"
     CDS             complement(417..584)
                     /locus_tag="ACHOTW_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9846700.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(581..1012)
                     /locus_tag="ACHOTW_004755"
     CDS             complement(581..1012)
                     /locus_tag="ACHOTW_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9846701.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(1182..1258)
                     /locus_tag="ACHOTW_004756"
     tRNA            complement(1182..1258)
                     /locus_tag="ACHOTW_004756"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1222..1224),aa:Arg,seq:tct)
     gene            complement(1359..1434)
                     /locus_tag="ACHOTW_004757"
     tRNA            complement(1359..1434)
                     /locus_tag="ACHOTW_004757"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1399..1401),aa:Ile,seq:cat)
     gene            complement(1574..2128)
                     /locus_tag="ACHOTW_004758"
     CDS             complement(1574..2128)
                     /locus_tag="ACHOTW_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP9846702.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPKLEAFLRDMINGKQKSWLVHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(2125..2415)
                     /locus_tag="ACHOTW_004759"
     CDS             complement(2125..2415)
                     /locus_tag="ACHOTW_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP9846703.1"
                     /translation="MVNLRKAAKGQICQIRIPGYCNHNPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACNSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKDGYL"
     gene            complement(2415..3014)
                     /locus_tag="ACHOTW_004760"
     CDS             complement(2415..3014)
                     /locus_tag="ACHOTW_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFP9846704.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDEV
                     EFQQVYKSVLNVLWNWILFRKFSSQEEVENVAAQLLEFA"
BASE COUNT          867 a          929 c          740 g          862 t
ORIGIN      
        1 tgtcattatt cctcccggcc gggatagtgt attaaatcag atatggagtg ggctggctgt
       61 agtccggaag cctgaatgac actcggggac tacagcccaa gaaatgaaaa aaggccacgc
      121 agttgcgcag cctgataaac cctggttaaa atccacacga taacaacaca acaatatcag
      181 tatctcatgc tattgcccga acccattcgg gcatttttta cccataaaaa agcccctccg
      241 gagaggggca tgtttgcatg cacattcttt ttcttgcatg gtgccgggtg cctcccggtg
      301 aattcagtat cagcacctga atccgcgatt atcccatata cctggttgct gatcgcccct
      361 ccgcacaggg ggattcacca tgcagaagtg tttttaataa acagcaaaca aaaaaatcaa
      421 gcattatgca ggctgtttct ttttatcacc ggccacagca ataccacaat gccgcagacc
      481 agcaccccat ccgccagcac cgacatgatt ctgctggtga aatccaccat caccaccaga
      541 aacagcagga gtgcagccac agccaggcgc agttttaccg tcacaggtga ttctccagac
      601 gaagacccag aacaccggca atctcttcca gcaccttgcg ctcttccggc tcaatttcgc
      661 cgtctgcctc cgcaatggcc accgccacat ccagcacatc ttccgcttca cgcgtatcgt
      721 gtttcacatc ctcaatctcg cgtaacgcgg cacgacgacc aattttaaaa ttggtatcca
      781 gctgaccgat aatagttgcg ctaatcgcat taatttccga ggtaaacgca gacaacgcag
      841 gctgattacg taagacctgt tcgatcttcg ctttctcgga tgcctcgcat tcaccatctg
      901 cataggccac caggtatgca gcgttaatca ccgcctgtgc cagatcgcgt ttctcaaact
      961 ttttaatttc cgctgccgct ctgcgggttt tctttttgaa gattccaaac atcgtgacgt
     1021 tcctttgggt gggtgagcca acgcccggga gcgatctgcc cacagagaaa gtcacactga
     1081 ccactccgta agctcacccc cgaaaggctc tgtggttgat atgcgccggg cgtggcgcag
     1141 atacaaaaaa ggcccgccga agcgagcctg gaaaataagt gtggcgcgtt gtagtggagt
     1201 cgaaccactg accgattgct tagaaggcaa ttgctctgtc cggctgagct aacaacgcag
     1261 aataccgata atggaccgcc atcggggacc cgcccccgca ccaacaaccc tgttatggtg
     1321 tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg gtggcccttg ctggatttga
     1381 accagcgacc tggcgattat gagtcgctcg ctctcaccac tgagctaaag ggccgggagc
     1441 agaataataa cggtccgtaa ttaattccgc aataaaaaac ccgctcaatg gcgggttctg
     1501 gtaaagttca tgcgtttggt tcgcctcgcg atacagcttt gcgaagctta ccggaattga
     1561 agcagttttt acgtcaaaaa gcaataactt ttttctctat accaaaagcc ataaccattg
     1621 gtttgtacaa aataaattct gccaccttta gccaatgctc aatgcgtctt tcacaggttc
     1681 ttaaactcca ttccggatgt gcatcattca gcagttcagc cattttgcgc ttagtcatcc
     1741 cccgccccac ataacgctga ctcagaacat tgagcagccc gggataacct gccagtactt
     1801 caccaataac cctgtcgatt attaacgcct ctgaatcggt acaatgcacc agccagcttt
     1861 tttgcttgcc gttgatcata tcccgcaaaa aagcctcaag tttaggtttg tccagacctg
     1921 cttttttcat cctccggagc gcctcgttaa ttgccgtttt tgtcagcttt ttagaggtca
     1981 gcaactggtt gaacatattt cccgtcttac cgccgccaat atacgaccag cgcccccaca
     2041 tacgtagttt cccctgaatc cagacacttt ccagcgtggt gagacgaagg tgctctccgc
     2101 tttttcctgt gttcgttggg taaatcataa ataaccgtcc tttctccaga tctcttgtgt
     2161 gcgaaaaaca ccttctgcat gcatcaggcg caattcttct ttggtgtaat cgctggtttt
     2221 tactctcccg tcgattaaat cgtggcatga gttacaggca atcgctgcct gcatatcgtg
     2281 tggctttatc gctgttccgc acgttcccgc cagcctgtaa tgcgccagca cagaggtttc
     2341 gggattgtga ttgcagtagc cagggattct gatctggcat atctggcctt tagccgcttt
     2401 acgtaaattc accattacgc aaactccagt agttgtgcgg ccacattttc aacttcctcc
     2461 tgagaggaga atttacggaa cagaatccag ttccacagca cattcagtac agatttataa
     2521 acctgctgaa actcgacttc gtccatattc gcaaaagcga tggattttgc ccgacgccca
     2581 cggctaccgt ccggataaat atgctcggtg taaaatccgg cctgaatggt tacccactcg
     2641 cggaaagcct caaacgactt tagcaacgcc gtatcccggg ttctacgagt cgcaacggtg
     2701 ttaaggtatt gctctgcggc atcactcagg gctggcgtgt gttcccgacc aactgattcg
     2761 cacaggtaat caacgaaacc agacagcagt tctcgttcgc gaggcgtgat cgccccaccg
     2821 accggagtcc agtaatcgaa tcccagttgc aggagtttga aaaaacgctt gtggaatgcg
     2881 tagttacgca cacgcttaaa atctgcgtgt atccactcac ctattttgat ttgatgcaga
     2941 aaatcgcaac tctccggcgt cgccgggaga agtaaaccag aagaagtttg tttgaccagt
     3001 tgtatatgcg ccatttctca atctctcgat ggcgcagtgc agcagatgcc agttgttcaa
     3061 gctagcacgc aaattgtaaa ccagaatgcc aggaaaaaac aaaacccgcc gaagctggtt
     3121 gtgtgcggtt gcgttgaagt ctcctagcca aagagtagcg gtgaccactc cccgccgata
     3181 atcttagttg tttaatttcg taagctaact aggctaaaag ctctctgttg tctgcctgtt
     3241 gtacaattaa caaaatgaac aacgaactag atataccaac gccagaatgg tgcaacaaat
     3301 cccaaacaac acagcaatgc aacaataaca tatgttattt tcctctcacg ggattcagtc
     3361 ctgaattctc ataaagctaa tcttttttta aaaacata
//
LOCUS       DAZKIF010000256         3388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZKIF010000256 DAZKIF010000000
VERSION     DAZKIF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.258"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..423
                     /locus_tag="ACHOTW_004761"
     CDS             <1..423
                     /locus_tag="ACHOTW_004761"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP9846705.1"
                     /translation="AASDKNALCAHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVE
                     KAAEQCIQQRQTVVMLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGN
                     SKGSMLLIWRPFISPRRMFTTVSKAALMAIGQGVRRAA"
     gene            420..602
                     /locus_tag="ACHOTW_004762"
     CDS             420..602
                     /locus_tag="ACHOTW_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9846706.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            877..1554
                     /locus_tag="ACHOTW_004763"
     CDS             877..1554
                     /locus_tag="ACHOTW_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFP9846707.1"
                     /translation="MAVQISVENLSPVTYNQIPVITTELLAHLYGTKIKNISDNFLNN
                     TTRFMPGKHYFKIEKNELREFKNRPETIGLVGKNARSLILWTERGAARHAKMLETDQA
                     WDVFEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVWLWDYANRSQALFRELYPAL
                     KLIQSGYSGICHDYGYKFSYIIGRARGVLINHTRDIDIYEPDGPTNLLAWERLKNKEL
                     PPSLHRY"
     gene            1629..2351
                     /locus_tag="ACHOTW_004764"
     CDS             1629..2351
                     /locus_tag="ACHOTW_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP9846708.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSQHGNVRISIERLAKRGVIQLP
                     SMQKVENKQTISPNKFTSVYIFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMERGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            2351..2956
                     /locus_tag="ACHOTW_004765"
     CDS             2351..2956
                     /locus_tag="ACHOTW_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9846709.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAATPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            2953..3147
                     /locus_tag="ACHOTW_004766"
     CDS             2953..3147
                     /locus_tag="ACHOTW_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP9846710.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            3140..>3388
                     /locus_tag="ACHOTW_004767"
     CDS             3140..>3388
                     /locus_tag="ACHOTW_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9846711.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMA"
BASE COUNT         1018 a          778 c          874 g          718 t
ORIGIN      
        1 gcagcgagcg acaaaaatgc tctgtgcgct cactggctaa ctgaggccga cgacgcgctc
       61 aattctgagt gggtaagcca cggtgcaatc tggaataacc caccgtacag caatatcagg
      121 ccgtgggtgg aaaaagccgc tgagcagtgc atacaacagc gacagacggt agttatgctt
      181 gtgccagagg atatgtcagt cggatggttc agcaaggctc tggagagtgt tgacgaagtt
      241 cgcatcatca ctgatggacg gattaatttt atcgaaccat cgacagggct ggagaagaag
      301 ggaaacagca aaggctccat gctgctgatt tggcgaccgt tcatcagtcc tcgacgaatg
      361 tttactactg tatccaaagc ggcattgatg gcgatcgggc agggcgtcag gagggcggca
      421 tgaggcgaca gcgacgaagt atcaccgaca tcatctgcga aaactgcaaa taccttccaa
      481 cgaaacgctc cagaaataaa cgcaagccaa tcccaaaaga atctgacgta aaaaccttca
      541 attacacggc tcacctgtgg gatatccggt ggctaagaca tcgtgcgagg aaaacaaggt
      601 gattgacgcg atgatttatt cggggctata ttcctcacgc gccagcaaaa tctggcgtcg
      661 ggattagcac cccggatatc gaaacggtgc ataaccgcgc tggcggtttt ttatgcgcta
      721 agcacagtca cattcgcgat ttatggcggg ctgtgtgggg gagccgaaag gctcgccgga
      781 tgtttcgacc ggtagtgcta accccgcaca gttcgccacc acgatgatta gcacctgacg
      841 gtggcgaggt aaaaattatc gaaacgcgag gtcattatgg ctgttcaaat ttctgtcgaa
      901 aacctttccc ctgttaccta taaccagatc cccgtaatta ctactgaact gttggctcac
      961 ctttacggaa caaaaatcaa aaacatttct gataactttc tgaacaacac gacgcgattc
     1021 atgcccggaa agcattactt taaaattgaa aaaaacgaat tacgcgagtt taagaacaga
     1081 cccgaaacaa tcgggttagt tggtaaaaat gcccgctccc tcatcctctg gacagaacga
     1141 ggcgcagccc gtcacgcaaa aatgctcgaa actgatcagg cgtgggatgt gttcgaaaaa
     1201 ctggaagact gctatttcag ccagtgcgag aaaaatactg gcaaacaaga gaagaagctc
     1261 aacgggcttt ccgcaaaaga aacagacagc cttgtatggc tgtgggatta tgccaaccgc
     1321 tcacaggcat tgttccgtga gttgtatccc gcattaaaac tgattcagtc tggctattcc
     1381 ggcatatgcc acgactacgg ctataagttc tcgtatatca tcgggagggc gaggggcgtt
     1441 ttaattaatc acacgcggga tatagatatt tatgagcctg acgggccgac gaaccttctg
     1501 gcatgggaaa ggcttaagaa caaagagttg ccgccttcac tgcatcgcta ctgacaattg
     1561 acaacttaac aaacccagct tcggctgggt tttttattgc tgaattttca atgtgagagg
     1621 acatgacaat gaatgagctg ataaatagca atgccatcaa aatgacaagc attgaaatcg
     1681 ctgagttggt gggaagccaa cacggtaatg tcagaatatc aatagaacgt ctggcaaagc
     1741 gtggggtgat tcaacttcct tcaatgcaaa aagttgaaaa taaacaaaca attagcccta
     1801 acaaattcac aagcgtgtat atattcgaag gcgaacaagg taagcgagac agcattattg
     1861 tcgtcgctca gttgtcgccg gaattcaccg ctcgccttgt tgaccgctgg cgagaactcg
     1921 aaggggcaac cgcgaaaata ccacaaacct tttctgaggc attgcgcctt gcggccgacc
     1981 ttgaagacca gaaggctgaa ctggagaaac agcttgctct cgcagcacct aaagttgagt
     2041 ttgccgatcg cgttggcgag gccagcggaa ttttgattgg aaactttgca aaggttgttg
     2101 gtattggtcc aaacaaactg tttgcgtgga tgcgcgatca caaaatcctt attgcttcag
     2161 gttcccggcg aaatgtgcca atgcaggaat atatggagcg cggctatttc acagtgaaag
     2221 aaacagcggt caatacaaat cacggaatac agatatcgtt caccacaaaa atcaccgggc
     2281 gtggtcaaca gtggctgaca agaaagctgc tagataacgg aatgcttaaa gtaacagggg
     2341 aggctgctta atggctaaac cagcgcgaag gaaatgcaaa atctgtaagg aatggtttca
     2401 cccggcattc tcaaatcagt ggtggtgctg cccggaacac ggaactcaat tagcactcga
     2461 acgacgaagt aaagaacgcg aaaaagcgga aaaagcagca gagaagaaac gacgacgaga
     2521 ggagcagaaa cagaaagata aactgaagat tcgaaaactc gccttaaagc cccgcagtta
     2581 ctggattaaa caagcccaac aagccgtaaa cgccttcatc agagaaagag accgcgactt
     2641 accatgtatc tcgtgcggaa cgctcacgtc tgctcagtgg gatgccggac attaccggac
     2701 aactgctgcg acacctcaac tccgatttga tgaacgcaat attcacaagc aatgcgtggt
     2761 gtgcaaccag cacaaaagcg gaaatctcgt tccgtatcgc gtcgaactga ttaaccgcat
     2821 cgggcaggaa gcagtagacg aaatcgaatc gaaccataac cgccatcgct ggactgtcga
     2881 agaatgcagg gcgatcaagg cgaagtatca gcagaaactt aaagacctgc gaaacagcag
     2941 aagtgaggcc gcatgacgtt cacagtaaaa accattcctg acatgctcgt tgaggcatat
     3001 gaaaatcaga ccgaggtagc cagaatactg aactgtagtc gcaacacggt cagaaaatac
     3061 actggcgata aagaagggaa aagacacgct atcgtcaacg gtgttcttat ggttcaccgc
     3121 ggatggggta aagatactga tgcgtgatat ccggcaggtt cttgagcgct ggggggcatg
     3181 ggcggcaaat aactatgagg atgttacatg gtcgcccatt gctgccggat ttaagggact
     3241 gatccccgaa aaagtaaaat cacgtccaca gtgctgtgac gatgatgcga tggtgatatg
     3301 cgggtgcata gcccgccttt accggaacaa tcgcgatctg catgacttgc tggttgatta
     3361 ttacgtgttg ggggagacgt tcatggcg
//
LOCUS       DAZKIF010000257         3362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZKIF010000257 DAZKIF010000000
VERSION     DAZKIF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.259"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            31..390
                     /locus_tag="ACHOTW_004768"
     CDS             31..390
                     /locus_tag="ACHOTW_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9846712.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            399..929
                     /locus_tag="ACHOTW_004769"
     CDS             399..929
                     /locus_tag="ACHOTW_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP9846713.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            1171..1368
                     /locus_tag="ACHOTW_004770"
     CDS             1171..1368
                     /locus_tag="ACHOTW_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9846714.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1519..2577
                     /locus_tag="ACHOTW_004771"
     CDS             1519..2577
                     /locus_tag="ACHOTW_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9846715.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            2618..2693
                     /locus_tag="ACHOTW_004772"
     tRNA            2618..2693
                     /locus_tag="ACHOTW_004772"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2651..2653,aa:Ile,seq:cat)
     gene            2774..2850
                     /locus_tag="ACHOTW_004773"
     tRNA            2774..2850
                     /locus_tag="ACHOTW_004773"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2808..2810,aa:Arg,seq:tct)
BASE COUNT          861 a          731 c          954 g          816 t
ORIGIN      
        1 actggcgtgc tcgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg
       61 ccgacggtga acacttactg gcgacgccgt ggcagcacat attttgtatc aaaagccggg
      121 gagcgttatc gccgggcagt ggtgcttatt gttcgccagc agcgactgaa attaagcctg
      181 tccggacggc tggcaataaa aattattgca gagccaccgg ataagcgtcg tcgtgacctg
      241 gacaatattc tgaaagcacc gctggatgcg ctgacgcatg cgggagtgtt aatggacgat
      301 gagcagtttg atgaaatcaa tattgtacgt ggtcagccag tatctggtgg acggctgggt
      361 gtgaagattt acaaaattga gagtgagtga gcgtaaatat gatatatccg gaaattacag
      421 gcaaaagcgg cgaacattta cgcctgaaca cgctggaagc agtctggatc caggggaaat
      481 tacggatgtg ggggcggtgg tcgtatatcg gtgggggtaa atccggaaat atgtttaacc
      541 ggttactggt ttcgaaaaag ctgacgaaaa cagcagttaa tgaggtttta cgcagcatga
      601 agaaatccgg gctggaaaaa ccggaacttg aggcattttt tcgggatatg accagaggga
      661 agcagaagag ctggttgtca cattgtacag acacagaggc gttgattatt gatcgcgtta
      721 tcagtgaggt gcttggggaa tatcccgggc taatcaatat tctccggcaa aggtacgaag
      781 gacggggaat gagtaagaga aaaatggcag aatgtttaaa tcgtactcac ccggaatggt
      841 gtttcagcac atgtgagaaa cgtattgcag gttggttagc cgtggctgaa cacatgcttt
      901 atgtacctat gcacgattca tttcgataaa aaaagcttgc ttttttacgc agaaacagct
      961 tgaattcctg taagcttcgc aaagctgtat cgcgaggcga aatgcaagtt ttttcgcaca
     1021 aggaagccac cggaaggtgg tttttttgtg tccgtaatat acagcagcgc aataaattcg
     1081 ctggtggtta ttaataccgt tctttcaggt tgctggcttt ttcgacaaga gttattggtg
     1141 tgtcacgtta accggaaaag ggaaaaagac atgctgaaac agcaggatat gacagaaacc
     1201 gccagagtgg tgtttaatga attaagcgtt accgacccgg cgacagtcgg ggagattgcg
     1261 cagaatactt acctttcacg cgaacgctgc cagttaatac tgacccagct ggttatggcg
     1321 ggtctggcag actatcagtt tggttgttac agacgccttc agtcctgaag gcttttttat
     1381 ttgtggtaaa tgggcggctg gtgggtgtaa ggggcaccca ccagccatct gctcatgcgt
     1441 tgggttcaca agcaaacctc aggcccactg ctttgcgcaa aagcagaatg agcctatcag
     1501 agacaggctt aatgatccat gcttaatact gtaaaaatat ccagttgtga gttaatcaac
     1561 gccgactgcc tggaatttat gcggtcgtta cccgaaaatt ctgttgacct gatagtcacg
     1621 gacccgccgt acttcaaagt gaaacccgag ggctgggata accagtgggc gggtgatgaa
     1681 gattacctga agtggctgga ccagtgtctt gcgcagttct ggcgggtgct gaaacctgcc
     1741 ggaagtcttt acctgttctg tggccatcgt ctggcatctg acaccgaaat catgatgcgt
     1801 gagcggttta acgtgctgaa ccatatcatc tgggcaaagc cgtccggacg ctggaacggg
     1861 tgcaacaagg aaagcctgcg ggcgtatttc cccgccacag agcgcattct gttcgcagag
     1921 cattatcagg ggccgtatcg tccgaaagat gccgggtatg aggcgaaggg taggacactg
     1981 aaacagcatg tgatggcccc gctgattgct tactttcgtg atgcgcgcgc tgtcctgggg
     2041 ataacggcaa aacagattgc agatgccaca ggaaagaaaa acatggtgtc gcactggttc
     2101 agtgccggtc agtggcagct gccgaacgaa agcgattatc tgaaattaca ggcactgttt
     2161 gcccgggtgg cagaagagaa gcatcagcgg ggtgaactgg aaaagcccca ccaccagctg
     2221 gtggatacgt atgcctctct gaaccgacag tatgcggagc tgcagagtga atataagcat
     2281 ctgcggcggt atttcggtgt gacggtgcag gtgccgtaca ccgatgtgtg gacgtataaa
     2341 ccggtgcagt actatccagg gaaacatccg tgcgaaaaac cggcagaaat gttgcagcag
     2401 ataatcagcg caagcagtcg tccgggagac ctggttgcag atttcttcat ggggtcgggg
     2461 tcgacagtga aagcagcgat ggcgctggga cgtcgtgcaa ctggcgttga actggagact
     2521 gaacgttttg agcagacggt gcgggaagta caggatttaa tcattcgtaa cggatgagat
     2581 tgcggagtta atcatgcgtc gttattattc agcaatcggc cctttagctc agcggtgaga
     2641 gcgagcgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca ccaaccgcca
     2701 ctagctcatc aggaaagaac gtcaccctgt gcgagattcg gagtccccgg tggcggtcca
     2761 ttatcggtat tctgcgttgt tagctcagcc ggacagagca attgccttct aagcaatcgg
     2821 tcactggttc gaatccagta caacgcacca caccacactt atctgccctg actctctttt
     2881 gcgggctttt tattacagga aagacaccgg acagtgaaat gttaaatgcc tcacaattca
     2941 ggcagttgac tgttgcctga catgctgagc gtttgttaaa aaaatcctgc atgatgaatc
     3001 cccctgagcg gcggggcata atgacagatg tttggttgcg tattgtatag gcaagttgcg
     3061 gattctgtct ggtcattgca gaattcaccg ggaggcaccc ggcatcatgc tgtatacaga
     3121 gattaggcat atatccaggc ttctcatcgc aggagccttt ttacatgcaa aaaaaagccc
     3181 gagtgggttc gggcaacagc atgagatact tgcattgtca tttttatcgt gtggatttta
     3241 accagggttt ataaggctgc gcaactgcgc ggcctttttc gttttgcggg ctgcggttct
     3301 cctcttttga ttctccttgt ggccggaccg tggcccgcaa ctgttgagga aaatcccgga
     3361 aa
//
LOCUS       DAZKIF010000258         3355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZKIF010000258 DAZKIF010000000
VERSION     DAZKIF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.260"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(601..798)
                     /locus_tag="ACHOTW_004774"
     CDS             complement(601..798)
                     /locus_tag="ACHOTW_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9846716.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(864..1134)
                     /locus_tag="ACHOTW_004775"
                     /pseudo
     CDS             complement(864..1134)
                     /locus_tag="ACHOTW_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1439..1627
                     /locus_tag="ACHOTW_004776"
     CDS             1439..1627
                     /locus_tag="ACHOTW_004776"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846717.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1955..2149
                     /locus_tag="ACHOTW_004777"
     CDS             1955..2149
                     /locus_tag="ACHOTW_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846718.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2492..2620
                     /locus_tag="ACHOTW_004778"
     CDS             2492..2620
                     /locus_tag="ACHOTW_004778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846719.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2930..3265)
                     /locus_tag="ACHOTW_004779"
     CDS             complement(2930..3265)
                     /locus_tag="ACHOTW_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846720.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT          996 a          767 c          734 g          858 t
ORIGIN      
        1 cagtttgtag gagctttctt ttttgaacaa tgatttgcag gacagttatg ctaaactagc
       61 ggaatcagta tcacaacaat aaatgactga tcatacctga aatttgaatt aacaggcatc
      121 atgaatgtca atagtctaag cggtgactac ttatatcgct tgcataaata ctaaacactg
      181 actagaaaag tattggataa ccccgtcact aaaagtaacg cttcggttat ttgctcggaa
      241 tgtattaacg ccaagattgt tagccgttac gccttatatc actggcgctg acaaatgttt
      301 cttttctaat ttactgaacc ggcgcagaca cgcaggaaag cacgccggca ccggtgtaat
      361 gccagttcta atcaataaga atggaatgac gaatcagtca ggaagggata acaacccgct
      421 gctgaacgga aggtatcagc aggaagatga acgggaatat cgccagcacc aagggcaggg
      481 ccgggttcca caataccagc gctggcattt tgcacattgc cagcgtaccg gcattacaac
      541 acgtttcatg ctgctgcccc ccaggattcc tcgattcgtt gctacatcca ctgctctact
      601 tcacttccac tgctccactt cacttttgta ccagcggctg ctgcgcccca gtttgatggg
      661 tttagggaaa tggccttcac tgatcaactt gtaaaaccat ttgtcggtca taccggtgaa
      721 actggtgata tagttcatgt cgatcaggga attatcggca cgcagaaata cgctgtcgtg
      781 gcttgttgta gtcaacatgg tcagaatgtc ctgtgagatt tatgaagatt gtgcgaatgc
      841 gggaaatcta ctgagctgtg ctttcagaac tggcctgtta cgggatacca gggattaccg
      901 gcggggtaac gggcttccgg atcatacaca ccacgattat cgcggacaaa atcactgaac
      961 gcccatatca cctctttaag tatgtcttcg cagtccggta aatgacgatc cagcgccaca
     1021 tcccgggtgg tacagctttg atgcgcccgg tgacacaaag cccggattgt tccagacatc
     1081 ctgaatcaaa cgccccagat taggggcgtc gaaatatgcc tctctgacca ttatattccg
     1141 gcgtataggt agcaggtctg aagtgacaat gcgtcacctg acgttaaaag tcacccaaga
     1201 tgaccccagt gggggcaaca agtataaaac cgttttttga ttaagtggtc ggatatgaaa
     1261 aggggaaggt ttatgtgaaa aatacctgtg ttttctttga aaacacacaa cagtatcagg
     1321 ttatttatgt ttatacagtg cgttccaaac tgacctgaat aattacgcaa gttgcaacaa
     1381 taaaacattt ttgccgtagt gtgaggtact ccggtaaatt gcttcccagg tgcagacaat
     1441 gactaacgaa gaactacagg caatcgcatt atccatgctt gagcgccagc gcgaaaaagt
     1501 acaccagcaa cgagagtctt tgcttaaccc atctccattc accaggccaa aacgattgga
     1561 gcttgaagca ttcttggaca aatacccacg gcggcttaaa tcaggcaaaa ccagaccacc
     1621 aggctgatag ctcacagttt gttctccgat tcaataaaac ggataagaac catcagcgcg
     1681 gtagcgtaag gcactacagc gcattgcttt acgagtaata acaatggatt tatcaccaga
     1741 agacgcaaag tttttacgag aactgcaaca ccgtatgctc agagaagcca ttatcgaaca
     1801 gcaaacgggc atgagcgcca tagacccatt gcgatacaaa cgggcctcat ctcctttagt
     1861 tgacgacaat tccaatgcgg ataaaacacg tccaccacac tgattacatt tttcattagc
     1921 acagtggtgt gatgcagcat acgaggaaaa ccggatgaat gagaacaaaa taaaaaggct
     1981 ggagcaactg ttacaggcac gccaacggga gtttgccact aaatggggac gtgccgcgct
     2041 gccacatgaa cgcctaacct attttccact tgaagacctc attaaaaata atggcaaaaa
     2101 aatcaggtct gataagaaac acttcttaga atgcaatgaa gacaaataac aacaatctgc
     2161 atattgccac caaccacaga tgtggcatcg ttatcagcgc acttgatttt ctttatatcc
     2221 actgacgagt ttgccggtca ttctggtatc atgcagatat ttacggcgta caagcacact
     2281 agcatgacat tgtaataagg cctgagccta acgctttaat gtgtgtcata cactcagtgg
     2341 cgtaatcgtt gacaatatgg tgcatgtaat cgtaagcgtc cagcgaaatc catctggtca
     2401 taatctgaag catccgacaa agtggtgtcc accaaataag tgatctgcgc ccaatatcct
     2461 gagccggaac tttgacaaag gccaacgaaa aatgggagcg gatgttacag agttctcggt
     2521 acaggggaaa agtggtacct gtcgccagtt ctccgatctg tttaaccggg aaattatctc
     2581 ctacagcttg tcagaaaggt cggtgatgga gcatcgttaa tacgatgcta tacgatgcat
     2641 tcacagtgcc cggccagagg atgccccgtc gctgcatatg gatcagggtt ggcaatatcg
     2701 aattgcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat gccgcacaaa
     2761 ggaaacggtc tgaataacgc agtgatgaag aacttcttca gcacactgct aaaacgcata
     2821 gtgatcgagc gctgattctg gcgaacaact gaactaatac atcagaatct gcattatgtt
     2881 aaataaatat aaaaagatgg tttaaatacc ccgttacttt tgacttacac tatacggtat
     2941 cgcatcgttt aatattcgca ccggccagat ttttatttct attagttgtc acaatactga
     3001 atgcgtacga ccacagtatt ctggctcctg tgtggttatg ctttaattct gcgttccggg
     3061 cagataagca gttgcttgca ggaatccttc ttgtgttaat gtcagttccc cttttaccag
     3121 tgctgatttc cacattccgt ccaacagagc ttatagcctt tccctggatt atagcattgt
     3181 ccggctgaag ttctttttga ataataatag aagcactgct ggcagatcca gtccgttttt
     3241 cataacccac tgtactgata accataatct aatcagtaga aattgagtcg aaaataagca
     3301 ctactccata caggataatt agaggtcagt ttgattattc acaattcatc atcag
//
LOCUS       DAZKIF010000259         3343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZKIF010000259 DAZKIF010000000
VERSION     DAZKIF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.261"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..162
                     /locus_tag="ACHOTW_004780"
     CDS             <1..162
                     /locus_tag="ACHOTW_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP9846721.1"
                     /translation="KRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQES
                     ALACWGIDR"
     gene            436..1131
                     /locus_tag="ACHOTW_004781"
     CDS             436..1131
                     /locus_tag="ACHOTW_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HFP9846722.1"
                     /translation="MKEMISVDHEISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            1226..1357
                     /locus_tag="ACHOTW_004782"
     CDS             1226..1357
                     /locus_tag="ACHOTW_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846723.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVVLFLSITWMALTSAGLVG"
     gene            1580..1765
                     /locus_tag="ACHOTW_004783"
     CDS             1580..1765
                     /locus_tag="ACHOTW_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9846724.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPS
                     PDWQTPLNGIISSSENG"
     gene            2166..2492
                     /locus_tag="ACHOTW_004784"
     CDS             2166..2492
                     /locus_tag="ACHOTW_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFP9846725.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(2624..2824)
                     /locus_tag="ACHOTW_004785"
     CDS             complement(2624..2824)
                     /locus_tag="ACHOTW_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9846726.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            2866..3231
                     /locus_tag="ACHOTW_004786"
     CDS             2866..3231
                     /locus_tag="ACHOTW_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9846727.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
BASE COUNT          832 a          723 c          928 g          860 t
ORIGIN      
        1 aaacgaatta atgcaggaga tcgaaaaggt gcctgcgaag cgattcgctg gtggattaag
       61 gacggtggca gagactgccg tattcgctca aataactgtt atggtcaggt atcccgtcgt
      121 gaccaggaga gcgcgctggc gtgctgggga atcgacagat aagcagaata ttttgctgaa
      181 aaatgacgtt ggccagcgcg ggcggataac acgaaatcct gcgaactggc aaaatgtaag
      241 tgaataaagt taggcagatt atttcacgca gaggcaccgt aatggtgcct ttgtcatttc
      301 tgcgcttcgc acaagcgtaa ataaaccaaa gaacctttca ggatgagccc tggtggataa
      361 ccggcagtgg tctggttaac cctctttggg ctggttattc ctgtgcgcag ggttcatcac
      421 taaaaggaat caaccatgaa agagatgatt tctgtcgatc atgaaatatc catgagtagt
      481 ctggattttc tgaataacat tattaatcca gcccgggcag aagccggaga agtccctcat
      541 gaaccgcgta agtttcttgc aaaaattgag gatgagctag agcttgatgg aaccggaaaa
      601 aaattccggt taaacaataa ccagacaaga acggcatact atgatctgga ttttgaccag
      661 atgatgctcg ttggcatgag ggagtcaaag gccgttcgtc gttctgtgct ggcaagactc
      721 aaagcgatgc atggtattca gatcccccgg actttacctg aggcgttgcg atttgcggca
      781 aaactggctg aacagaaagc agtgctggaa aatcaactgg caatagcagc gccgaaggct
      841 gaatttgttg ataactatgt tgaagcatct ggtctgatgg gattccggga agttgctaag
      901 ttactcggta tcaaagaaac cgatttccgg ctgtttttgt tggagaacgg aataatgtat
      961 cgcctcgctg gaaaaatgac gccttactcg catcacctgg atgcggggcg gtttagcgtg
     1021 aaaacgggcg aggcgggcaa tggtcatgct tttacgcagg ttaaattcac cccaaaaggt
     1081 gttcagtgga ttgctggtct gcttgctgca tggagagcta ccgcagcatg aagatgataa
     1141 aaacggactg gaaatttttg ctggtctggc tgattccgtt tttatgggtg gttgcccggt
     1201 taattactgc tattaagggg taaagatgtc agacaaactc ataatgctgg cgaagggcct
     1261 ttgtgtaatc gtcggtattt cattttcact aatgctggtt gttctttttc tttccataac
     1321 ctggatggcg ttgacttcgg cagggctggt ggggtgagca taaaccgaat gctttccgcg
     1381 tttaccgtta ttctgctggt ggtctgtggt gcgcttagtc tggggctgaa tcattaccgc
     1441 gataacgtca tcacctacaa agcgcagcgc gataaaaaag ccagagagct ggagctggca
     1501 aacgcaacca ttactgatat gcaggtgcgc cagcgcgatg ttgctgcgct cgatgcaaaa
     1561 tactcgaggg aattagccga tgcgagagct gaaaatgaaa ctctgcgtgc tgatgttgcc
     1621 gctggtcgta agcgcctgcg gatcaacgcc acctgctccg gtaccgtgcg tgaagccacc
     1681 ggcacctccg gcgtggataa tgcaaccgtc ccccgactgg cagacaccgc tgaacgggat
     1741 tatttcatcc tcagagaacg gttgatgaca atgcagaagc agctggaagg ggcacaggac
     1801 tatatccgca ctcagtgcct gaactaagtt ttgctgatgc gccgtatcgt cgctgtattc
     1861 cctcattaac agagaccgca gcccgacagg gagactcctc tgcgcgagtg tgcggggaga
     1921 atcaaaaacg atacgcaccg gggtttaccg cgttaacgga gcgcggcgtt gtcccctcat
     1981 ggtcgctggt tcggtgcgat ggtggaagaa accggacgat gtgttacctc gcaagacctg
     2041 ttatgtcatg tgtctgattt gtgatttaag tcggataatt gtcgttgcca ttaagcagag
     2101 gattgatggc cgacagggtg gcattgttag aataagactt attcttatct gtgccgggaa
     2161 tgaaaatgaa aagaaatctt ccgttaatta ttttgttgtc ttctctggtt atgggctgta
     2221 cgcaacataa aacagatatg ccccgacagt tggttaaggc attaccacaa tatccggcct
     2281 atgcagcggc aaattatata aagggacggg ttgatgtgaa gtttgatatt ggtgctgatg
     2341 gtactgtcac ccgaattgag tttatccgtt cagagccgca ccatctgttt gatgagcagg
     2401 ttgtaaaggc gatggcaaaa tggcgatttg agaaggacag gccgcgtaaa ggcgtgaaga
     2461 aaacgtttat ctttagtcct tctgcaccct gattatttca tcagaaatta attatcactc
     2521 tgttgttatt ctgtacattc cggctgggta agtcttgttc cgccgggtat gaagatgaaa
     2581 tattgttgga ggacagtggg tacctgctcc tgtaaccgaa cgttcatttc tcgttatttg
     2641 tcatgctggc cggacgcaga tgcgttgcat ctgttgccag ccttctcctg caggcttcaa
     2701 taacccacgc tgaaaagtta ccggaacctt tatgttcaag ggcgatattg atctgttcaa
     2761 tcatgtgatt ggggaaacgg atattgcggg ttgtggttct gcgggtccgg tttttcgatg
     2821 acatatttat ttcctttact gattgccata tgacggggat tttacatggc tgagcttcgt
     2881 acactccaga gcagaatcaa aacactgaat acccgacggg tgaatattct gaagggtgaa
     2941 cagcgtcgtg tcagtggcag tgcacgtgtt tccctcaagc gtcatatctg gctcagggat
     3001 gccgggcagt gctgtctctg tggtcgtgtg gttgacctct gtgacagtga actcgatcac
     3061 cgaattgcac ttcagttcgg tggtggtaat gaggagacga atctctggac gctctgtacc
     3121 gaatgccatc gacaaaagtc tgctcgtgaa gcggcgggtg gtatgccgga cccgacgctg
     3181 ccggaggtgt ccggaggtag tggcagagcg gacgacatca tcggactgta acccgagcgg
     3241 gggggtatca tccggcgtaa aaaacgatcg ctccggacac cgcgccccct ctcacgcaga
     3301 gaaaaaattc ccgtttcagg gcagttaaca tgttaactgg ctg
//
LOCUS       DAZKIF010000260         3326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZKIF010000260 DAZKIF010000000
VERSION     DAZKIF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.262"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..384
                     /locus_tag="ACHOTW_004787"
     CDS             <1..384
                     /locus_tag="ACHOTW_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9846728.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRDRGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASP
                     KCPQGPTPIELLKQEYERRKAAGFV"
     gene            418..1134
                     /locus_tag="ACHOTW_004788"
     CDS             418..1134
                     /locus_tag="ACHOTW_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9846729.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            1131..1448
                     /locus_tag="ACHOTW_004789"
     CDS             1131..1448
                     /locus_tag="ACHOTW_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9846730.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            1445..1747
                     /locus_tag="ACHOTW_004790"
     CDS             1445..1747
                     /locus_tag="ACHOTW_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP9846731.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            1737..2054
                     /locus_tag="ACHOTW_004791"
     CDS             1737..2054
                     /locus_tag="ACHOTW_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFP9846732.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            2008..2325
                     /locus_tag="ACHOTW_004792"
     CDS             2008..2325
                     /locus_tag="ACHOTW_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846733.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            2312..2749
                     /locus_tag="ACHOTW_004793"
     CDS             2312..2749
                     /locus_tag="ACHOTW_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9846734.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            2751..2942
                     /locus_tag="ACHOTW_004794"
     CDS             2751..2942
                     /locus_tag="ACHOTW_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846735.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            2945..>3326
                     /locus_tag="ACHOTW_004795"
     CDS             2945..>3326
                     /locus_tag="ACHOTW_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846736.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPD"
BASE COUNT          920 a          693 c          992 g          721 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgtgacaga
      181 gggattgagc ggcagatgac ggaaggtgag ttaaaacgac ttgcagaacg gcaactgacg
      241 aaatgggcaa agcaagttgg taacgggatg agtattcccc cgatccggag acaactcgca
      301 tcgccgaagt gcccgcaagg gccaacgcca attgaattac tgaaacagga atacgagcgc
      361 cggaaggcgg ctggttttgt ttgaatctga gaaacgattt tgtcggagga aattttaatg
      421 gaaaccgtat ctgacgcact gaaagcactg aaaagagcct cttcacatgt ggtggcagct
      481 cgccttggaa ttagtcgtga agaggctgtc aacgaactgt ggaaactgaa acgccgtgga
      541 gaagcggata acaagggggc gatatggtgg ctgactcagg ctggtgaaag tgaaccggtg
      601 tcaccggtac cgaaagtgac ggcgcaaatg ctgacagagg cgattgaaca acatggccca
      661 caaacggcgg atgaactggc attgatgttc ggaattacct cccgccgggc gaattcatcg
      721 ctggcaatgg caatcagcaa agggtgtctg attcgcgtga atcaggatgg taaatttcgt
      781 tactgcatac cgggcgttga tttaccggca gagccgaaag cagcatccgt agctgaaacg
      841 gagggtaaag cccttcctca gccagctggt gttgcgttac cagtccagga aacggctgca
      901 caggaagaaa ttaaaacaga agcggtggag gacattgtga agttgcagca atcgttcact
      961 gaagcgaaag cagatgacct gattctacca tcgctgcatg tggctaaccg cgagctgcgc
     1021 cgggcgaaaa gtaatgttca gaagtgggag cgagtctgtg ctgcgctacg ggaactgaac
     1081 aaacacaggg atattctccg ggatattacc gccaccagag agcagcagcg gtgagtggct
     1141 ggaagaagtg gcgctgggct gaaatcctga tactccggca gtgtgcggga acgatgagag
     1201 tcgaaagcat cggttatctg attggccgta gtgagtcagc cgtcaggacg aaagcgcggg
     1261 aactgggtat cagcatgatg ttacggggtg attatcacca gtcagccaaa tgttcacagc
     1321 gtgatattga gctggcgtgg caactgcatc agcgtggtgt accccgacgg gaaattgccg
     1381 aaaagtttgg gatgaagttg ggcgcagtga ataactacgt ttatttcgac aggagggttc
     1441 aggagtgagg gtgagggttt atatcgccgg tccgatgacg ggatatgaaa attttaaccg
     1501 cgaggcattt cacaaaacgg aagaggtgct gaaacgggaa gggcataccg ttttaaaccc
     1561 ggcagtactt ccggacgggc tgactcagcc acactacatg gatatttgca tggcaatgct
     1621 ccgttgcgtg gatgcggttt acatgctgaa aggctggcag cagtcggcag gtgcaggggc
     1681 tgagctggca ctggaggaga aaccgggcca tgcggtgatt tttcaggagg tgggcagtga
     1741 atattgaccc ggcgataacg attgatatgg ccctgaacgc cggcctggca cttcttggtt
     1801 atttctacat tatgttctgc agcggacgat ggctgtcact gttgttcatg aaaaaatgga
     1861 ataaacgccg taagcaggag caacgccaga aggcaatgga tgcatttttc gaagccttcg
     1921 gaattgacgg catggaacca ggggatccag ctcgcgcaat cagcagaggg ggtgtagtaa
     1981 tccttgtata tcggagtgaa gagaaaaatg acgatcacaa aacaacgtgt agaagaaatc
     2041 atatcccgca ttgaaatgta tggacatggt gcagggtata tcgcagacga ggttaatgat
     2101 ctggctatac tggcgctgaa tttatcaaat atcgcgaacc tgaagcgata cgagcttgat
     2161 atgggaggtt gcgactcgtg cggtcaggat tgtggcgctg atatgactga agatcctgat
     2221 ggtgattatg tcctgtttga tgacgtggtt aagttgtttg aatttgatac aaccactcaa
     2281 aagttagaaa tcccggcaaa ggaggcagcc agtgggcaag attgactatc aggcactgcg
     2341 tgaggcagca caaaactatc aatcgacgct ggcgtggtat caggctaccc cggacagccc
     2401 aaatgctgaa cgggattgtg atgcggctct tgctgcgttt aagcgtcaca tccgtcatcg
     2461 ggaagcggat attatcgctg atttgctgga tggactggaa gaagcaaaat cacaactcaa
     2521 cgagcagcgt gagtattacg aaggcgttat ctctgatggg agcaagcgta ttgctgaact
     2581 ggaagcgcgg gaagttcaat taccgactcg ctacgacctt cgatatggac acccgataaa
     2641 tgcagatgag cgacatgtca tgatacctaa agaaaatggc agttggcttt acctgattga
     2701 cctagaacac gcattacgcg tcgctggcat tcgcatcaaa ggagagtgag atgaacggac
     2761 aaatatcaat tgttcgacca ggagcatgtg acgatcgcga aatacgaatg attattcgtc
     2821 tggcgatggg gaaaacaata actgctctca ttactccaga aaatctcgca ttagcattaa
     2881 caggaaagtc agacatgcca gtagagctaa agctgcgaaa tgttgagatt aaggtgaaat
     2941 agctatgacc actattacca aagatcgact gctgacaatc cagcattggc gcgaaacata
     3001 cggaccgggt agcaacgttg tgcttccagc agaagaagcg gaagagctgg cacggattgc
     3061 gctggcatca ctggcagcag tatcggatga acgagcagcc tatgaattat ttatggagaa
     3121 gcgtttcgga gaatctgtag atcgccgcag agcaaaaaat ggcgatagag attacatggt
     3181 atgggatatg gcgcttggct ggattatctg gtgtcaccgc gccgccatgc ttcaggctgg
     3241 aaactttcgg gaaaataagg gttcgtcaac caataatttt cgggaaatct cggaaacgtc
     3301 aaccaactat ccggtaactc cggatg
//
LOCUS       DAZKIF010000261         3306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZKIF010000261 DAZKIF010000000
VERSION     DAZKIF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.263"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..572)
                     /locus_tag="ACHOTW_004796"
     CDS             complement(<1..572)
                     /locus_tag="ACHOTW_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9846737.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQA"
     gene            complement(668..856)
                     /locus_tag="ACHOTW_004797"
     CDS             complement(668..856)
                     /locus_tag="ACHOTW_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9846738.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            complement(853..1041)
                     /gene="dicB"
                     /locus_tag="ACHOTW_004798"
     CDS             complement(853..1041)
                     /gene="dicB"
                     /locus_tag="ACHOTW_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9846739.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            complement(1522..1674)
                     /locus_tag="ACHOTW_004799"
     CDS             complement(1522..1674)
                     /locus_tag="ACHOTW_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9846740.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            complement(1949..2593)
                     /locus_tag="ACHOTW_004800"
     CDS             complement(1949..2593)
                     /locus_tag="ACHOTW_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9846741.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
     gene            2691..2918
                     /locus_tag="ACHOTW_004801"
     CDS             2691..2918
                     /locus_tag="ACHOTW_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9846742.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
     gene            2915..>3306
                     /locus_tag="ACHOTW_004802"
     CDS             2915..>3306
                     /locus_tag="ACHOTW_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9846743.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSG"
BASE COUNT          860 a          756 c          766 g          924 t
ORIGIN      
        1 tctgcctgaa tttcaggcca tttagcgcca ggctcacatt tatgcttaac ccacccgatg
       61 gcatgcagtt taagttcagg atacatggcg ttaacttctg gcattttcat caacgcttca
      121 acgatatgtc cgccgaatgt tgccatgtct tcctgtagta attcctgtgc gctaatcacc
      181 atatcaacgg tgatgttttc gcatgtgtcg aatttaacca ggactgcgtt ctgtacttca
      241 ggggacagct tgtcaaaatt gacgttcatc ggatcggatt ctggttcgac cggtacaaag
      301 gaagctgact cctcatccca gcggttttcc tgcatatatt cggtatccca ggaatcgagg
      361 gcagggcggg gtatgccggg tttatcctcg cagacaataa atttataagc gcagtcctga
      421 gctgccggga attgctccag aaattgccag tgaaattttg cgcgtgcgcg acgctcatca
      481 ccggcttcaa tggcagtggc caccgcaaca gcgctatctt cttttatggc ctgttcgtca
      541 ggaatagcag cgcaaataaa gattttactc attgtgtttt aacctcatta cagatttcag
      601 ggtgaacgaa tccctgccat tgctggcatt tttaatccgt tggtatggcg ttaatatggc
      661 tggcgggtta tccagccggt gtttcgttat tcaggtacag cgatactttt tttaccggta
      721 ggcattcacc agaaattttt tgctcgtctc ttgcctggag gcaggattct ttactggcat
      781 aaattccggt aataacattc tgtgattcac ccgttataag aaaaaccgtc atcaccagtg
      841 caaatgctga agtcattgac gttctccgaa aataccaagt ttaataaggg caattcggga
      901 aagaatggaa ttatcattga gcagataagg ctcatatttc cgcatattaa tggcatcctc
      961 agtaaacgct ttattactga gcagaacacc aatatcaaaa caaccttcag acgtattaac
     1021 gtttggtaat aacgtttcca ttatcgcgtc ctcaacaatg aattttgtga tgcagtgcct
     1081 ggtgcctcca ggtgacgtta accagttaac aattaacgtc gggttagttg atgctcgtta
     1141 cgccagtaaa agaccgcctt actgctttaa ctgttccgcg tgcgcatagc cgcattcacc
     1201 gcatcacaaa attcacttta aaaagggcgg atatccattt ccgccgaatc accagaaaag
     1261 tgataacaga ggttgttgtg gctggggtgt cacttaagcg tatggtcaac ctgacaaccc
     1321 ggtgtcctca acgggggaag gaataacccc gccatactta ccgccgcgcc atttcgcgga
     1381 ttgccacaac cagaagcgca cggtcgaatt aaatttaacg acgacctata cagagagact
     1441 aacttcgccg tgcgctttcg tgttatgccc tgacttttca gggtaaatta acctgtggaa
     1501 acctgttttt actggcgcta atcagttagc gtttcttgct aaccagcgat gcgcgccagc
     1561 ttcggtttta aacgttttac ttctggtata cgtcatcgcg gtaaacgtgc cgtcgtggtt
     1621 ggggaatact ccgcacacca gagattcatt gttgccaaga ttgagcatat ccatgttgac
     1681 ctcatttacc cttaacgccg ggtcgcggaa ctaaaaacct gctgcgctgt tatacaaagt
     1741 gttcccgccg tcatgttcat acgcctcggg ctggctactt aacccctgac cactgccggg
     1801 taactcgagg tattgcccga cgttctgtgg ggcgaggtag ggtggtatgt aataaatcta
     1861 aaagtattta gtttttcagt caaggtaaat ctagattaat ttaaaaaaaa ggtcggcatt
     1921 gccgacctca agtaaaggac gtggcgggtt ataagttgaa ctgtacacct tttgctacgg
     1981 caacaatttt acactctggt gtaagtaagg atgactgata gcgtggattg agagggctta
     2041 aatacacaag cttcccatca ataactaatt tctttatagt catagatggt tcatttttaa
     2101 gcggatccgg aactattaca gcgacgatgc tgccattttt ataactttct cctggcctta
     2161 ggatcacagt agcaccaact gggatactcg gtgatcctga tgggttatgc attgtgtcat
     2221 caggcattga aacggcaaaa tcaccttcct cgacatcaaa gaatgtggtg attctatcga
     2281 catttcccat tgttttctct ccttttaaga ttaggaaaga aatcgcctca ccccacgaaa
     2341 ggtaggggat cttggtgcct gaggtacttt gtacaaaaga taattcagga gacgatactc
     2401 catataggag ataggactca gtagttccta atgcctgggc taacttactt agagctttgc
     2461 tacctggttc gtttagatct ttctcccagt accctatagt aaccccagtc acgcctgaaa
     2521 gcttacccag ttctacttgg gtcagcccct tatcttttct aagtctctta agcctgaagc
     2581 caaggctttc catcattttc tcctgcaaat tgaatataaa ttattttaga ttgcattgac
     2641 ctaaaaaaaa ttatcttgta atctaaaaat acttagattt gggaggtaaa atgcgagttg
     2701 atgaacttgt ccagtttttt ggttctgttc agagggttgc cgatttttat gggataaccc
     2761 gcgaagctat ttacatgtgg cgtaagcgtc ctggtgaaat agttccgaag gggagggctg
     2821 cggaagctgc tgcatactcc aaggggaaat tatctttgaa cccagaactt tacaaaaaga
     2881 aggataccac tcagggcgaa ggaaaggggg attcatgaaa atcaaacatg agcacatccg
     2941 catggcgatc aatgcctggg cctatccgga cggtgagaaa gttcctgcag ctgaaatagc
     3001 ccggacttat ttcgagctgg ggatgacgtt cccggaactg tacgacgaca gccatccgga
     3061 agccctggct cgtaataccc agaaaatttt ccgttggctg gataaagaca cccctgatgc
     3121 tgttgaaaaa attcaggctc tgttaccggc gatcgaaaag gcaatgccgc ctctgctggt
     3181 ggcccgtatg cgcagtcaca gctctgaata ttaccgtgag atcgtcgaac ggagggatcg
     3241 gctggtgaaa gatgtggatg attttgtcgc agcggcgatc gcctggggca ccctgactaa
     3301 cagtgg
//
LOCUS       DAZKIF010000262         3299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZKIF010000262 DAZKIF010000000
VERSION     DAZKIF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.264"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            232..1164
                     /gene="rfaD"
                     /locus_tag="ACHOTW_004803"
     CDS             232..1164
                     /gene="rfaD"
                     /locus_tag="ACHOTW_004803"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP9846744.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1174..2220
                     /gene="rfaF"
                     /locus_tag="ACHOTW_004804"
     CDS             1174..2220
                     /gene="rfaF"
                     /locus_tag="ACHOTW_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP9846745.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2224..3216
                     /gene="rfaC"
                     /locus_tag="ACHOTW_004805"
     CDS             2224..3216
                     /gene="rfaC"
                     /locus_tag="ACHOTW_004805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP9846746.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3213..>3299
                     /locus_tag="ACHOTW_004806"
     CDS             3213..>3299
                     /locus_tag="ACHOTW_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846747.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITT"
BASE COUNT          835 a          809 c          863 g          792 t
ORIGIN      
        1 cattcaaatg cttccgttat ttatcataca tcggaatatt gatactaaag cactattcac
       61 atgcaaaacc aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt
      121 agcatccttg cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca
      181 gacattcgtg tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc
      241 gttaccggcg gcgcgggctt tatcggcagc aacatcgtta aagccctgaa tgataaaggc
      301 atcaccgata ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg
      361 gatctgaata tcgcagacta tatggataag gaagacttcc tgatccagat tatggctggc
      421 gaagagttcg gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc caccaccgag
      481 tgggacggca agtatatgat ggataacaac tatcaatact ccaaggagct gctgcactac
      541 tgcctggagc gtgaaatccc gttcctgtac gcttcttccg cagccaccta cggcggacgc
      601 acctccgact ttattgaatc ccgcgagtac gaaaaaccgt tgaacgtcta cggttactca
      661 aaattcctgt ttgatgaata tgttcgccaa atcctgccgg aagcgaactc gcagattgtt
      721 ggcttccgct atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc
      781 gtcgctttcc atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt
      841 agcgagaact ttaaacgcga cttcgtctat gttggcgatg tggcagatgt aaacctgtgg
      901 ttcctggaaa atggcgtttc cggcatcttc aacctcggca ccggtcgtgc ggaatccttc
      961 caggctgtag ctgatgctac gctggcttat cacaagaaag gccagatcga atacattccg
     1021 ttcccggata agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg
     1081 cgcgcggcgg gttacgacaa accgttcaaa accgttgctg aaggtgtaac ggaatacatg
     1141 gcctggctga atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct
     1201 tgggttggcg acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc
     1261 caggcgataa tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg
     1321 gaagttaacg aagctattcc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc
     1381 cgcaaactgg gtcatagtct gcgtgaaaag cgctacgacc gcgcctacgt cttacctaac
     1441 tccttcaaat ccgcattagt gcctttcttc gcgggtattc ctcatcgcac cggctggcgc
     1501 ggcgagatgc gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg
     1561 ctaatggtgg aacgctatgt cgcgctggcc tatgacaaag gcattatgcg cacagcacaa
     1621 gatctgccgc agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat
     1681 acctgtaatc aattttcgct ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg
     1741 gagtttggtc cggcaaaacg ctggccacac taccactatg cggagctggc aaagcagctg
     1801 attgatgaag gttatcaggt ggttctgttt ggctctgcga aagatcatga agcaggcaat
     1861 gagattcttg ccgctttgaa taccgagcag caggcatggt gtcggaacct ggcgggggaa
     1921 acacagcttg atcaagcggt tatcctgatt gcagcctgta aagccattgt cactaacgat
     1981 tctggcctga tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtccg
     2041 agtagcccgg acttcacacc gccgctatcc cataaagcgc gcgtgatccg cctgattacc
     2101 ggctatcaca aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac
     2161 attactcccc agcgcgtact gaaagaactc aacgcgctat tgttacaaga ggaagcctga
     2221 cggatgcggg ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc
     2281 gcactcactg atgcccagca ggcaatccca gaaattaagt ttgactgggt ggtggaagaa
     2341 gggttcgcac agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata
     2401 cgtcgctggc gtaaagcctg gttctcggcc cctataaaag ctgaacgcaa agcgtttcgt
     2461 gaagcgctac aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc
     2521 gcggcgctgg ttacgcgtct ggcacatggc gtaaagcatg gcatggactg gcaaaccgct
     2581 cgcgaacctt tagccagcct gttttacaat cgtaagcatc atattgcaaa acagcagcac
     2641 gccgtagaac gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc
     2701 cagggcgatt atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa
     2761 tatgccgtat ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg
     2821 cgagaattga ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg
     2881 ccgcatgagg aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg
     2941 ccgaagatga gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg
     3001 gtggatacgg ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat
     3061 ggaccaaccg atccgggatt aattggtggg tatggggaga atcaagtaga gtgtcgttct
     3121 acaagtatgt ctcttgcaga tttgccagct caaaccgttt ttcaaaattt aaatcttgaa
     3181 ataataacca ataagttgac atcggagata agatgacctc aacattattt ttctctctcg
     3241 agaaaaaaaa ctggatagcg tactggaaca gagctctcgt attcttattc attaccacc
//
LOCUS       DAZKIF010000263         3286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZKIF010000263 DAZKIF010000000
VERSION     DAZKIF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.265"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            138..932
                     /gene="hyxR"
                     /locus_tag="ACHOTW_004807"
                     /pseudo
     CDS             138..932
                     /gene="hyxR"
                     /locus_tag="ACHOTW_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            1086..1847
                     /gene="hyxA"
                     /locus_tag="ACHOTW_004808"
     CDS             1086..1847
                     /gene="hyxA"
                     /locus_tag="ACHOTW_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFP9846748.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            1889..2971
                     /locus_tag="ACHOTW_004809"
                     /pseudo
     CDS             1889..2971
                     /locus_tag="ACHOTW_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2961..3149
                     /locus_tag="ACHOTW_004810"
     CDS             2961..3149
                     /locus_tag="ACHOTW_004810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9846749.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
BASE COUNT          929 a          690 c          809 g          858 t
ORIGIN      
        1 caaccaaatt aaaaacaata aaaatcaaac aaattagatt tatattccaa gaaaattaaa
       61 tattcaggca attaaacaaa acctgacatt ttgaatcaaa atgtcaggtt ttgtagctat
      121 ggcgtggacg agtctgaatg aagaagcctc tggttattat cagtgcctgt cagtttacca
      181 ggctggcact ggagtctctc atcccagcgg acaggtatat cgtgcgggtg tacagcaacg
      241 tcactacaga ggttgaattc gttttaaaga cctcctgtgg ctatctgttg gctgatatcc
      301 cttagcttcc ttcgggggag ttagttcttc tggcttgtct tgtccgcttg cggagtgatg
      361 ccgggcaatg gcaggtctgc ctgaccggtg actccgactg gttcgataat gctcctgctg
      421 caagtctgta taacggcttc ccacgcatcg gcaccatgct gacagcgagt cgttatcaga
      481 atcaaattat ccgctggtta ctacgtcctt tgcccggcga tatgagcgat ttctggctcc
      541 tgactgcacg ggagttgagc gtgcttaaga ttctgatgca gggcatgagt ttcagtgaga
      601 ttgcccgtta tgaacaacgc agttcaaaga ccctgcatgc gattgccaca cgggcattaa
      661 tgaaacttgg actgggaact ctgagcgatt tcaggctgct ctatacggga tgcggtgaca
      721 gcagagtgaa acggaatata cggctacatg caagatatgt cggacaacat tccgctcatc
      781 gactcactca gtatgtgcag aacatggtat ccggaattct ttatccagag aaagttgccg
      841 ttggtgaatc aataaattct gacgccattt ccggaaaagt acttcccgga caaagaaaaa
      901 atatttctaa aaaaagacca ggattgttat gacacaacag cgttgttact accccccact
      961 gcggcgcgta cggactcgct acagtgtagg ggccaaaata tttctactgc tctgggctgc
     1021 aggattggga ggcacgctgt acagcctgta cagcgtgctg gcgtactgac atctgtgagg
     1081 gaaggatgcg tttcgttctt ttctgtccct caggaattgt accagcgcag ttcgcagctc
     1141 tatcaaccgg ttctgtcggc aatgttacct gcatcggaac agaagaggag ttacgcaata
     1201 aattgcggca caggccacag agtgtcgtga tatcggcagg gcgacctgcc gaatgcgctg
     1261 aaatgtggtt ccgattttat cgggatcact cattcgtggt ggtgttgtgc gtcgcgccat
     1321 tttttctgcc accggatgtg agtatttctg gcgttctcaa aaacctgagg ttactgaaac
     1381 caggaatgtc agtagagcat gtcatcagta ttgcaaattc ctctggtggg ttatcagggc
     1441 tgaaacatgc agaaaatttg ccagttatgg acagttactc tgtctttatg aaagaggtga
     1501 ataaccgcac aaagacaatc gtcatgtcag aacgcttccc ggaaaagcaa aagaaggtgt
     1561 tgtcgttgct tcttgccgga cacagctggg aatacagtgc gcagtttctc aaaacgggga
     1621 taagacagat atggcttgcc gagcagtcgc taaaaaagcg ttggggaata ccggacagca
     1681 tgtcattgcg ggaagccctg ctgctgagca gcaataaatt tcacggtgat ggaaatgcat
     1741 tggaaaaaac gaatgccatg acattgcgtg aaaacggaaa taccaacagg tattccgtgg
     1801 tggtaaatgc agggacgcag gttttatctc tgcataaata taaataagtg ggaaattgaa
     1861 attagttacc agaatggaga atttcttcat gaaaaacagt aaggcatttt accgcagcgc
     1921 attagcgaca gctattgtta tggctctttc tgcaccagca ttcgctgctg ataacgcggt
     1981 atcaactgat tcggttacgc tgaataaaga taagacgact ctggatcaag atgttgttat
     2041 tagcaataca gcagacaaac agattacagc cgtaacaatt aatgcggcag atgaagacct
     2101 taatgttgct tttgctggtc atgatattac tgctgaatca acggcagaca aaaaattcgt
     2161 tgaaggtgta aaagttagtg gtaacaaaaa tgttgtgatt aatgctacag actccaccat
     2221 cacagctcaa ggtgaaggca cctatgtccg gactgcaatg gtcattgatt caactggcga
     2281 tgttgttgtt aatggcggta atttcgttgc aaaaaatgaa aaaggtagtg cgacagggat
     2341 atctctggaa ggggccaagg gaaataatgt cacgcttaat ggcacaacca taaatgctca
     2401 aggtaataag agttccagca acgcctccac ggcaattttt gctcaaaagg gtagcttatt
     2461 gaatagtttt gaaggtgatg caaccgacaa cattaccctt gctggctcaa atattattaa
     2521 tggtcggatt gaaacaatag ttactgccgg gaataagacg ggaattcata cagtcaacct
     2581 gaatattaag gatggctcag taattggggc ggctaataat aaacaaacaa tttatgcctc
     2641 tgcttcggca caaggcacag gttcagcaac gcaaaattta aatttgtctg ttgctgattc
     2701 aaccatctac tctgatgtcc tggccctttc tgaaagcgag aattcagccg ctaccacaac
     2761 aaatgtaaac atgaacgttg cccgctctta ctgggaaggt aatgcttata ctttcaatag
     2821 cggcgataaa gcgggtagta atctggatat aaatctttcc gatagttcag tctggaaagg
     2881 caaagtttca ggggcaggaa atgccagtgt atctctgcaa aacgagtctg tctggaatgt
     2941 tacgggatcc tcaactgttg atgctctggc tccgtctggg tgatgagtct gtcaatgcca
     3001 gcattgacgg tgcagcattc cgcgtgggtg caggtgtaca ggctgatatc accaaaaaca
     3061 tgggagcata tgcaagcctt gactacacca aaggtgacga cattgagaat ccgctacagg
     3121 gtgtagttgg tatcaatgtg acctggtaag aataaagcag tcggttcccc tgtcgactgt
     3181 tataccccct gcaggcagca gtatgctgcc tgcaggctat aaatatccag tattcatgat
     3241 gctggctttt tgctgatatt taacccgaac attcctgata tagact
//
LOCUS       DAZKIF010000264         3187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZKIF010000264 DAZKIF010000000
VERSION     DAZKIF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.266"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            55..330
                     /locus_tag="ACHOTW_004811"
     CDS             55..330
                     /locus_tag="ACHOTW_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFP9846750.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(410..523)
                     /locus_tag="ACHOTW_004812"
                     /pseudo
     CDS             complement(410..523)
                     /locus_tag="ACHOTW_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
     gene            complement(558..776)
                     /locus_tag="ACHOTW_004813"
                     /pseudo
     CDS             complement(558..776)
                     /locus_tag="ACHOTW_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            868..2096
                     /locus_tag="ACHOTW_004814"
                     /pseudo
     CDS             868..2096
                     /locus_tag="ACHOTW_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            2107..2262
                     /locus_tag="ACHOTW_004815"
                     /pseudo
     CDS             2107..2262
                     /locus_tag="ACHOTW_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein GogB"
     gene            complement(2639..>3187)
                     /locus_tag="ACHOTW_004816"
     CDS             complement(2639..>3187)
                     /locus_tag="ACHOTW_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFP9846751.1"
                     /translation="PYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHS
                     IKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMA
                     QQQVRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVE
                     PLAIIDRTFGMKENISMVNASK"
BASE COUNT          942 a          724 c          729 g          792 t
ORIGIN      
        1 caacttataa cggaatagca taaaaacact tttcatggag caaaggagaa aacaatgcca
       61 ttttcaatca aaaacagatt ttcaagttca caagtacatt acccggaaat atccggtccc
      121 ataaaagaca agccagcgtc aaagaactgc atacttacat caacaacatg taatgtagat
      181 agctatacag tgtaccaaaa aaaagcctgt agttttgaca tgcgcccacc cggcgcagga
      241 gaaagaaccc caaaactaaa actctcagtt actgagatga catggctatc taaaactata
      301 gaaacagaga tacacaacac aaaagaatag caaccactta ccagagaaca caaaaagcca
      361 cagacccgaa acacctgact gcaagcaacc acctccacag gaggtggttt taccatgaac
      421 ttcctgtgta ctgacttgtg ttcataataa tatttttgtg tttaaactca ataaagtcac
      481 aaaaatgatt gtaatcatgc aatgtagtaa aattaaaata tttgaccctg tacacgattc
      541 tgtgtaaatg ccttttctca gaagtgaccg tccaggcggt caccgaactc gataataaag
      601 cggctcattg ccatacgcca gtcccgcagt ggcatcgtcc atttctgtga agccgcctgg
      661 attgccagcc acacgacctt tttcactgag tcatccgtcg ggaacacctt gcgttttttg
      721 atggcatgcc ggattacgct gttcagcgac tcgatggcgt tggttgtgta gatgactaga
      781 ctggccccct gaatctccag acaaccagta tcacttaaat aagtgatagt cttaatacta
      841 gtttttagac tagtcattgg agaacagatg attgatgtct tagggccgga gaaacgcaga
      901 cggcgtacca cacaggaaaa gatcgcaatt gttcagcaga gctttgaacc ggggatgacg
      961 gtctccctcg ttgcccggca acatggtgta gcagccagcc agttatttct ctggcgtaag
     1021 caatactagg aaggaagtct tactgctgtc gccgccggag aacaggttgt tcctgcctct
     1081 gaacttgctg ccgccatgaa gcagattaaa gaactccagc gcctgctcgg caagaaaacg
     1141 atggaaaatg aactcctcaa agaagccgtt gaatatggac gggcaaaaaa gtggatagcg
     1201 cacgcgccct tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt
     1261 cgcgtgcgca gttgtacgtc attctcagac gaaccgatga ctggatggat ggccgccgca
     1321 gtcgtcacac tgatgatacg gatgtgcttc tccgtataca ccatgttatc ggagagctgc
     1381 ccacgtatgg ttatcgtcgg gtatgggcgc tgcttcgcag acaggcagaa cttgatggta
     1441 tgcctgcgat caatgccaaa cgtgtttacc ggatcatgcg ccagaatgcg ctgttgcttg
     1501 agcgaaaacc tactgtaccg ccatcgaaac gggcacatac aggcagagtg gccgtgaaag
     1561 aaagcaatca gcgatggtgc tctgacgggt tcgagttctg ctgtgataac ggagagagac
     1621 tgcgtgtcac gttcgcgctg gactgctgtg atcgtgaggc actgcactgg gcggtgacta
     1681 ccggcggctt caacagtgaa acagtacagg acgtcatgct gggagcggtg gaacgccgct
     1741 tcggcaacga tcttccgtcg tctccagtgg agtggctgac ggataatggt tcatgctacc
     1801 gggctaatga aacacgccag ttcgcccgga tgttgggact tgaaccgaag aacacggcgg
     1861 tgcggagtcc ggagagtaac ggaatagcag agaacttcgt gaaaacgata aagcgtgact
     1921 acatcagtat catgcccaaa ccagacgggt taacggcagc aaagaacctt gcagaggcgt
     1981 tcgagcatta taacgaatgg catccgcata gtgcgctggg ttatcgctcg ccacgggaat
     2041 atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa atataggggc
     2101 aaatccagat ggatttatcg taataaagac aacagccaaa taatgaaaaa cataaaattt
     2161 tatctgcatg gcaaagagat accagcagaa agaatattag atacaccaga gtggaaagac
     2221 taccgtccaa aatactccgg ttccacatat aaatattctt aatgatagca aaaaatatat
     2281 tttcgatata atcaatgtta tgattaaaga gtatttcatc agggcaggta aaaacagagt
     2341 aaatcagcaa aagaagctga tcttcagcga tactgacact aactgacggt ttaagcggtc
     2401 gtatgaagca gcagctttcc gacggactgc catgcggatc gtttaccttt tgggctattc
     2461 cgcccgtcat caagcggctc acgagtactg agtttatcag gatgatattg ctgacaatgg
     2521 taattcgttg accgatgtgt acttcactac atatcggtca acaccgtact gccgtgcaac
     2581 atctccaaga tgtagcatcg tttttaccag aggaggcagg acatagattg taaaaaaact
     2641 atttcgatgc atttaccatt gagatatttt ccttcatacc aaaagttcta tcaattatcg
     2701 caagtggttc aacaggtgcg gtcttatcta taacccaacc actctctgta acacttcctc
     2761 gtgtgatata ttttgccttt actttaacaa ctattagata atgctcacga ctaaaccgat
     2821 cagcaacgct aagatccgat gtgtattcag gcaatattct tccaatacgt acctgctgtt
     2881 gtgccatata ttcatctggt gcttttacat ttggatttga atcaggaagc cctccagcag
     2941 ttttatattc aacgatattt tcagcttgaa taccggttgt gccgcgacac aatatgatgt
     3001 ctgaatcgga cagttctatg tgcccctcct taattttcga ataaatttgt ttaatactat
     3061 gttctggagt tggtactatt tcagaagata aagcaggcaa cgtgtcttca agcaccggag
     3121 aatcatcgta tatatccaac aaatcctcat catacccaat atctccttct cttaactgag
     3181 aatacgg
//
LOCUS       DAZKIF010000265         3187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZKIF010000265 DAZKIF010000000
VERSION     DAZKIF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.267"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            328..1305
                     /locus_tag="ACHOTW_004817"
                     /pseudo
     CDS             328..1305
                     /locus_tag="ACHOTW_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1825..3187
                     /locus_tag="ACHOTW_004818"
                     /pseudo
     CDS             1825..3187
                     /locus_tag="ACHOTW_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
BASE COUNT         1085 a          640 c          643 g          819 t
ORIGIN      
        1 tatgagaaca ccaataagaa taataatatt tgatcacaac tgaagatgct ttttacaatg
       61 aagaattatt gttaaaaaac gctttgttga atcaaccgaa ctgttgctga gttgcaggat
      121 cacatcacgt attttcctga aaacgcaggc tgcttagtag caaagcagaa gttcaaaatc
      181 accaactaaa tagctgcgcc gaatagtaga tcaatgaaag ggaactcagc ccggattgtg
      241 cgatctgatc aatcgccaaa aatccccccc taaccggact gagcgatgcc gatcatagca
      301 ccaattcccc gcgacgaatg acgcctgatg caaaaagcaa tctataaaac tcacgataaa
      361 aattatgccc gcaggctgac cgccatgctg atgctgcacc ggagcgctcg cgtcagtgac
      421 gttgccagaa cgctctgctg cgcccgttcc tccgtcaggc gctggattaa ctggttcacg
      481 ctgtcgagtg ttgaaggtct gaaatcatta cccgcaggac gttcccgccg ctggccgttt
      541 gagcatatct gcacattgtt tcgtgaactg ataaaacact ctcccggata ttttggctat
      601 cagcgttcac gctggagtac agaactgctg gcaataaaaa tcaacgagat aaccgagtgc
      661 cagttacatg ccggaaccgt tcgccgctgg ttgccattag tcgggcttgt gtggcgcagg
      721 gctgcgccaa ccctgcgtac ccgtaacccg cataaagcag caattcacaa agcactggac
      781 gaatgtagtg cggagcatcc ggtcttttat gaagatgaag tgtatatcta tcttaatccc
      841 aaaaccggtg cggactggca acttcgcaga cagcaaaaac atgtggtaac accggggcag
      901 aatgaaaaat actatctggc tggtgcttta catagtggaa cgggcaaagt cagctatgtg
      961 ggtggcaaca gcaaaagctc agccttattt atcagtctgt tgaaacatct gaaattgaca
     1021 taccgacggg ataaacctat cacgctcata gtggacaact acatcatcca taaaagtcgg
     1081 gaaacacagc gctggctgaa agaaaatccg aagttcagag tgatttatca gccagtttac
     1141 tcgccgtggg tgaatcatgt tgagcggtta tagcaggcgc ttcacgatac gataacgcgc
     1201 aatcatcagt gccgttcaat gtggtaactg cttaaaaatg ttcattattt tatggaaacc
     1261 gtgagcccat tccccggtgg taaatatggg ctgttaaaag tgtagcggta ttcggcgcag
     1321 ctatttagga tggtcaggtc gcagaagttc tgtgccacta cagtgcgtgt actaaacaaa
     1381 atgacgaggg cacgaatgtc ataatacgtg cgtattacct gacagtagct accaactgag
     1441 ggggcttatt ccaaacctga tttattcaac aaagctggac ttatccttac tctgaattat
     1501 tttccagaca tatgcatcat gtactggctc gcttggttca ggaaatacag gagcaaatct
     1561 acagctatta gatttattcc tatttgtatg caaagtcgca ttgcataatg cggagctatg
     1621 ttacagatac gcctgacgtg cgtagtaaaa ttataaagca acatatatat acaaggagaa
     1681 accgagaaaa ataaatgagt cgtatgttgg gtagcatcga gataaatatt ttttttatca
     1741 cattagcacc ttggttaagg gtgttaataa taatattctc tcacttatta aaacataaat
     1801 aattttttaa gcggaaatgt aattatgaga ctgccagaga aagttctttt tcctcctgtc
     1861 actagtggcc tgtcagggca ggaaaaacaa aaaaaaccga agagcattac cggatttcag
     1921 gaaaattatc aacgcaatat caggccaatc aaaacagcat cagaagcccg actacgcttc
     1981 tttgataaaa tggtttcgaa agaaaactct ctggaagatg ttgtttcttt aggtgaaatg
     2041 attcagaagg aaatttatgg gcatgaacaa agaacatttt caccagttca tcatacaggt
     2101 aactggaaat catcattgtt acacaacgcg ctccttggtc tggcaaatgt ttataatggc
     2161 ttacgggaaa cagaataccc taacactttc aacagagatg gtataaaaag tactaactct
     2221 tttagagata acttattgac aaaaacaaga actcccagag ataattttga ggaaggaata
     2281 aaacatcctg aacatgcaac aataccatat gacaacgaca atgaaagtaa taaattgcta
     2341 aaagcaggaa agatagctgg taacaataac gagctgttga tggaaataaa aaaggaatcc
     2401 caaagcgacc atcaaatccc cctgtcagat aagttcctga aaaggaaaaa acgatctcct
     2461 gtagctgaag ataaagttca aaactcgtta acaccagaaa attttgttca gaaaatttca
     2521 cttagtgatg agcttaaaac aaaatatgca aatgaaattc tagagataaa aagaataatg
     2581 ggagaataca atcttttacc ggataaaaac agtcgtaatg gtctaaaact tctacagaag
     2641 caagctgatt tactaaaaat aatcatggag gatacctctg ttacagaaaa tacctttaaa
     2701 aacatagaga tggctatagc agatataaaa agggagtact attcgcatac agttgatatt
     2761 gagaaaaata ttcatgccat atgggttgcg ggttccccac ctgaaagcat ctcggactat
     2821 attaagactt ttctcaaaac ctacaaagag tttacttact atctttgggt tgatgaaaaa
     2881 gcatttggag ccgcgaaatt taccagtgtc ttaaaacaaa ttgcatttga tttagcatgc
     2941 agaactatac agcagaatac tccacaaaaa aatattgatt ttattaatct atataatgaa
     3001 ataaggaaga aatacaacaa caacccatcg ggacaacaag agtacttaaa caaactcaga
     3061 gagctttatg ctacttatca aaaaatatcc ccccctctga aacacatgtt taattcattt
     3121 ttttagaaaa catgattaaa cttcaggata atttcttcaa ctattgcatt gtcaaaggtg
     3181 ttacaga
//
LOCUS       DAZKIF010000266         3184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZKIF010000266 DAZKIF010000000
VERSION     DAZKIF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.268"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..510)
                     /locus_tag="ACHOTW_004819"
     CDS             complement(<1..510)
                     /locus_tag="ACHOTW_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9846752.1"
                     /translation="MAFKHYDVVRAASPSDLAKRITQKLKEGWQPYGGALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAVCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKK"
     gene            complement(997..1266)
                     /locus_tag="ACHOTW_004820"
     CDS             complement(997..1266)
                     /locus_tag="ACHOTW_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFP9846753.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1278..2237)
                     /locus_tag="ACHOTW_004821"
     CDS             complement(1278..2237)
                     /locus_tag="ACHOTW_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFP9846754.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            complement(2328..2404)
                     /locus_tag="ACHOTW_004822"
     tRNA            complement(2328..2404)
                     /locus_tag="ACHOTW_004822"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2368..2370),aa:Arg,seq:tct)
     gene            complement(2418..2494)
                     /locus_tag="ACHOTW_004823"
     tRNA            complement(2418..2494)
                     /locus_tag="ACHOTW_004823"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2458..2460),aa:Arg,seq:tcg)
     gene            complement(2504..2579)
                     /locus_tag="ACHOTW_004824"
     tRNA            complement(2504..2579)
                     /locus_tag="ACHOTW_004824"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2544..2546),aa:Ile,seq:cat)
     gene            complement(3021..>3184)
                     /locus_tag="ACHOTW_004825"
     CDS             complement(3021..>3184)
                     /locus_tag="ACHOTW_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9846755.1"
                     /translation="DTCIGKRLHKAEGIVEGMLMMLGVRLEMDRYVERELPGGRTSVF
                     YQRKNSLRS"
BASE COUNT          847 a          824 c          690 g          823 t
ORIGIN      
        1 ttttttggcg atatgcagcc cctgccccac ggttccgtac tgcccctttg acaggtccgc
       61 tttcggatgg ttaagacggc tcatgtcctg cacatcatgc agacaatggt ccgccggaat
      121 gatgtcgtta tatttgcata ctgcaccgcc cggtgtcacc gtactgcgac gcgctaactg
      181 tttaatacgt ggatcagggc tgtcgaatgt atccggcaat ggcagtccct caccgtatga
      241 cataccattg gactgaccag caagcgcgat cacatagtaa tattctggcg caacagaagg
      301 cgctgaggtc gtcggacggt tgcctggctc ctctggtgat gagatgctcc cctcactcac
      361 aactggctgg atgaactccg caccataacc agctgtcgaa atcagcgcac caccataagg
      421 ctgccaccct tccttcagtt tttgagttat tcgtttcgca aggtctgacg gcgacgccgc
      481 cctgacaaca tcatagtgtt taaatgccat gaatcctccc ggccgggata atattgtgag
      541 taaaataagg agcgggctga agtccggaag ttacaggaca atggcagaag gaagactaca
      601 gcccgaaata cgaaaaaggc cgcgcagttg cgcagcctta tgaatcctgg ttaaaatgat
      661 ttcgattatt aaaacgagta tctcatgcaa ttgcccgaac ccactcgggc ttttttacat
      721 gtaaaaaggc ccctgctatg agaggccttg gtatatgcct aatctctgta tactgcatgg
      781 tgccgggtgc ctcccggtga gttcggcctg gtaccaccga acccgtgtcg ataatgaatc
      841 acaagcaagg atttttcacc agtcgcccct ccacacaggg ggattcacca tgcgaaattt
      901 ttttaacaaa tgctcagtct gacaggcaac tgtcaactga ctgaattgtg acacagatta
      961 cacttgttac ccacatacca cgaatcaggt tatgcctcag tcattattaa actgcacttc
     1021 agcaaatccg gagcctgatt cacaggtact ggatttgatt gtgacagtca ttcctgtcaa
     1081 ctgagcactt tgcagtaacg gttgcagatt ccagcgactg gtccagtatt ctttcccgtc
     1141 aaccttcact gtaaatgtgt catcctcatt atacttggaa aactcaattt tacctttagc
     1201 acaatccgcc gccattgcat taacagaagc taatgcaaat aaaaccgcca taaacatctt
     1261 cttcatgctt aactccttta tttacccgtt gtatataaaa actgtgactt tctgttcaga
     1321 aacgctgcag ctgtattact ttcccataat gtattgttta tttttataac aggcctgtcg
     1381 ccagttatct gacattctgg ttgactctct tcattcacgg cgcgaacaga acgcgccccc
     1441 tgatgatggc aattcagtat aacggccaca gtccccagta tcgctgatat attattaaag
     1501 gatattctcc ccactctgac accatcctct ccccgatact ccggaagcac attgctgatt
     1561 cgcccccagt tcagagtgag gtccacgtct cccggcgtca tcgtatacac aggagcagtt
     1621 tcagacagtg cctgacgaaa ttctctctgt atctgcctga agcgtaaggc ttctgctgtg
     1681 acagtgacaa aacgcagaac tgctctggat gcatctctgg tcattgtatt accactgaac
     1741 tccattaacg ccagatatga tgaaaccagt gagtgacgac tgatttgcat tccggaacgt
     1801 tccagcgctg cgacacgttg cagagtggta taactgctgt ccgttgtcat ggaaaccgtt
     1861 gtcacaccgg gcactgatat atgtgtaaaa tctgaaaaac ggtagaaagt atttgttgcc
     1921 gtattaacga acccggccac atataaatta ttttgctcaa taatcagacg aagatggtca
     1981 aaacgcgcct gatagacatc aagccctcgt atatccacag caaaataact gcccggtggg
     2041 gtgtggttaa taacagacac cgatgtggtc ccctgagata tatgttcaag aggggtcgat
     2101 atctctgtcc gtatactatt taacgaagag acataacttt gttgggtcga aaagtctatc
     2161 gtaaactccc gggaatagga taccgaagaa aaacccagta acaggcacag tacccattta
     2221 aataatatac acttcatata caggtgttcc ttttggctga agtaatcagc accagacccg
     2281 gcgcagatat aaaaaaggcc cgcaaaagcg agcctggtaa ataaatatgg cgcgttgtac
     2341 tggattcgaa ccagtgaccg attgctcaga aggcaattgc tctgtccggc tgagctaaca
     2401 acgcataatg cagataatgg accgccatcg aggactcgaa ccccgcgcag ccagcttcga
     2461 aggctggcgc tctgtcccga tgagctaatg gcggtatgtg atatggtggc ccttgctgga
     2521 tttgaaccag cgacctggcg attatgagtc gctcgctctc accactgagc taaagggccg
     2581 ggcgcaggat aataacggta cgtaactaat cctgcaatat catccgttct gactgactac
     2641 attctgaact tccctgaccg tctgctcaaa acgcccgctc tccagctcaa cgccaattgc
     2701 acgacgccct agcgccattg ctgctttgac gctacggaca taaaaaagcc agccactggg
     2761 ggaggctggc aaactcgtag agcaaaatgc tgttacgcaa acttcgttac agggttatcc
     2821 tgcaatactt aaaatataca atatttagaa aactaatagt gctatatgcg atttttaaga
     2881 ttttgttatt aattacggtc gcaccttcct ttctgtgtac tttccgtata gctcacagga
     2941 ttcggggtac aaaaaaaccc gcgcatcggc gggttcgact gcgtggcaat gtaaccactc
     3001 ttatcatgat atgcagattt ttacgatcgt aaactatttt ttcgctgata aaatacagag
     3061 gttctccctc ccggcaattc acgctcaaca taccgatcca tctcaagcct cactcccagc
     3121 atcatcagca tgccttcaac aatcccctcc gctttgtgaa ggcgtttacc tatacaggtg
     3181 tcag
//
LOCUS       DAZKIF010000267         3170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZKIF010000267 DAZKIF010000000
VERSION     DAZKIF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.269"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(16..91)
                     /locus_tag="ACHOTW_004826"
     tRNA            complement(16..91)
                     /locus_tag="ACHOTW_004826"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Val,seq:tac)
     gene            complement(136..211)
                     /locus_tag="ACHOTW_004827"
     tRNA            complement(136..211)
                     /locus_tag="ACHOTW_004827"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(176..178),aa:Val,seq:tac)
     gene            470..1885
                     /gene="gltX"
                     /locus_tag="ACHOTW_004828"
     CDS             470..1885
                     /gene="gltX"
                     /locus_tag="ACHOTW_004828"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9846756.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(2003..2428)
                     /gene="tnpA"
                     /locus_tag="ACHOTW_004829"
                     /pseudo
     CDS             complement(2003..2428)
                     /gene="tnpA"
                     /locus_tag="ACHOTW_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            2436..>3170
                     /locus_tag="ACHOTW_004830"
     CDS             2436..>3170
                     /locus_tag="ACHOTW_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9846757.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANI"
BASE COUNT          826 a          772 c          827 g          745 t
ORIGIN      
        1 tagctggtga gaaagtggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag
       61 ggaggtgctc tcccagctga gctaatcacc cacttctcaa tttcttgcta cacggcggag
      121 actacataaa gtagttggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag
      181 ggaggtgctc tcccagctga gctaatcacc cccgctgtgt ggagtcgcat tatagggaga
      241 gttcaaaatg agtcaacgca ttttctaaag aaattgttcg ttcgtcgtaa atttaagcaa
      301 gatgatcgca aaacagaccg tgttgcgcaa tttgtcaacg aaaacaataa tgcgtaaggt
      361 tgcaacccga actacattga ggaatcaggc gggagtgata gaatatcgcc cactcaattt
      421 ttccaggatt tgccggttgt cggcatcttt ctaaacgtaa ggccatttca tgaaaatcaa
      481 aactcgcttc gcgccaagcc caacaggcta tctgcacgtt ggcggcgcgc gtactgctct
      541 ttactcctgg ctttttgcac gtaatcacgg cggtgagttc gtgctgcgta ttgaagacac
      601 cgatcttgag cgttccacgc cggaagctat cgaagccatt atggatggca tgaactggct
      661 gagcctggag tgggatgaag gtccgtactt ccagaccaaa cgttttgatc gctacaacgc
      721 ggtgatcgat cagatgctgg aagagggcac agcttataaa tgctattgct ctaaagagcg
      781 cctggaagcg ctgcgcgaag agcaaatggc gaaaggtgag aagccgcgtt atgacggtcg
      841 ctgccgccac agtcatgagc atcacgctga tgatgagccg tgtgtcgtac gttttgctaa
      901 cccgcaggaa ggttctgttg tttttgacga tcagatccgt ggtccgatcg agttcagcaa
      961 ccaggaactg gacgatctta ttatccgccg taccgatggt tccccaacct ataacttctg
     1021 tgtggttgtc gatgactggg atatggaaat cacccacgtt atccgtggcg aagaccatat
     1081 caacaacacg ccacgccaga tcaacattct taaagccctg aaagcgccgg taccggttta
     1141 cgcgcacgtt tctatgatca acggcgatga cggcaaaaaa ctgtccaaac gtcacggggc
     1201 agtcagcgta atgcagtatc gtgatgacgg ttatttgcca gaagcactgc tgaactatct
     1261 ggtgcgtctg ggctggtccc acggcgatca ggaaatcttc actcgtgaag agatgatcaa
     1321 atacttcact ttgaatgccg tcagcaaatc tgccagtgcg ttcaacaccg acaagctgct
     1381 gtggctgaac catcactaca ttaacgcgct gccgccggag tatgttgcta ctcacttaca
     1441 gtggcacatt gagcaggaaa atatcgatac ccgtaacggc ccgcagctgg ctgatctggt
     1501 gaaactgctg ggcgaacgct gcaagacgct gaaagagatg gcacagagct gccgttattt
     1561 ctacgaagat tttgctgagt tcgatgccga cgccgcgaaa aaacatctgc gtccggtagc
     1621 gcgtcagccg ctggaagtgg ttcgtgacaa actggccgcg attactgact ggacagctga
     1681 aaacgttcat cacgctattc aggcgacggc ggatgagctg gaagtgggta tgggtaaagt
     1741 tggtatgccg ctgcgtgtcg ccgtaaccgg tgcggggcag tctccggcgc tggacgttac
     1801 cgttcacgcg atcggtaaga cccgcagtat cgagcgtatc aacaaagcgc tggcttttat
     1861 tgcggaacgc gaaaatcagc agtaattagc gcataaaaga taaacggcag gagagatacc
     1921 cctgccgttt tttatttatc cggtgcgccg taaaaccccg tccttcaggg cggggatata
     1981 aggcgcggtt ttccacctga ccaggtgttt gctgttgttc aatgtattgg cggatgacgg
     2041 atattggcgc acctccgcag ctactggcaa aatagccagg actccacaaa acgcctttgt
     2101 agtaatacct gactgcaata tctggtcgat ctcgtcgcag taacctaccc gatacgcctt
     2161 tgaggctgtt taccagactg gatatcgcca gtttgggagg atagttagtt aacaaatgga
     2221 cgtgatctgg ttcgccatcc atttcaacca gttcagcttc aaaatcagca catacatttg
     2281 aaaagtaagt gcgtagtttt tctgtcgcgt catagtcaaa aatctgacgt cggtatctgg
     2341 tgacaaagaa cagatgaaca tgcatcagga aaacacaatg ccttccccgt cggatatcgg
     2401 tttctttatt catagaccaa agtatcattt ggcctgtgaa acgactacag gcatttaaat
     2461 tccagttaag accaggtggt caacaggagc gtgaaatgag gcgcttcgca ggcgcatgtc
     2521 gtttcgtttt caatcgtgct ctggcgcttc agaatgagaa tcatgaggcc ggtaataaat
     2581 acatccctta cgggaaaatg gcttcctggc tggttgagtg gaaaaacgcc actgaaacgc
     2641 aatggcttaa agatgccccg tcacagccat tgcaacagtc actgaaagag cttgagcggg
     2701 cttacaaaaa cttcttccgg aagcgggctg cttttccccg attcaaaaag cggggacaga
     2761 atgatgcatt ccgctacccg cagggcgtta agctcgatca ggaaaacagc cgtatttttc
     2821 tgccgaaact tggctggatg cgctacctga acagccgtca ggtcacgggt gttgtgaaaa
     2881 atgtcactgt cagccagtcc tgcggtaagt ggtacatcag tattcagaca gaaagtgaag
     2941 tatcaactcc ggttcaccct tcagcatcaa tggtcggact ggatgctggc gtggctaaac
     3001 tcgccacgct gtcagatggc acagtctttg agcctgtaaa cagttttcag aaaaaccaga
     3061 agaagttggc gagacttcag cgacagttaa gccgcaaggt caaattcagc aataactggc
     3121 agaagcagaa acgcaaaata cagcgactgc attcctgtac cgcaaatatc
//
LOCUS       DAZKIF010000268         3127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZKIF010000268 DAZKIF010000000
VERSION     DAZKIF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.270"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(167..363)
                     /locus_tag="ACHOTW_004831"
                     /pseudo
     CDS             complement(167..363)
                     /locus_tag="ACHOTW_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
     gene            complement(650..1468)
                     /locus_tag="ACHOTW_004832"
     CDS             complement(650..1468)
                     /locus_tag="ACHOTW_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP9846758.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALFFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(1620..1991)
                     /locus_tag="ACHOTW_004833"
     CDS             complement(1620..1991)
                     /locus_tag="ACHOTW_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9846759.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(1981..2352)
                     /locus_tag="ACHOTW_004834"
     CDS             complement(1981..2352)
                     /locus_tag="ACHOTW_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9846760.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(2365..>3127)
                     /locus_tag="ACHOTW_004835"
     CDS             complement(2365..>3127)
                     /locus_tag="ACHOTW_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9846761.1"
                     /translation="HGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQN
                     LAAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSV
                     YRESDIIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDPESPKSFMKR
                     PKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHH
                     NELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT          966 a          795 c          590 g          776 t
ORIGIN      
        1 accatgataa ctgaacagac aacaaccatt ataattttac tttcattagc aattggttag
       61 ttcaattata gccccaaaag gtaaattatc atcaacacat aagcaaagga ctgacaggtg
      121 ccgctaacac ccaccagccg cccatttacc acaaataaaa aagccttcag gactgaaggc
      181 gtctgtaaca accgaactga tagtctgcca gacccgcata accagctggg tcagtattaa
      241 ctggcagcgc tcgcgtgaaa ggtacgtatt ctgcgcaatc tccccgaccg tcgccggttc
      301 ggtgacgctt aattcatcaa acacaactct ggcggtttct gtcatatcct gctgtttcag
      361 catgtctttt taccctttcc ggttaacgtg acacaccaat aactcttgtc gaaaaagcca
      421 gcaagctgaa agacctgtat taataactac cagcacattt aacgcactgc gctactttgc
      481 gggcacaaaa aacccgctca gaggcggggt caagctatgc ggcgaaataa ccactcttaa
      541 cagcatacct gattttttac gtacgtaaat gctttgccgt gcatattttt catgcaaatg
      601 tcacgtcctg ctatttttca gtcttataaa tttaaaacca tagaaaaaat caattatgtt
      661 ttaaaaatgg ataggtaaag aataacaagt gacacagatt caaaccaaaa tggaaaaggg
      721 tggcaaccca caaacgccca ctccacatcc atgacaatcc atacacaaca ccagataacg
      781 tggcaaataa tacaagtaaa gcaccacctg aatagtgata aaaaccaaac aacaaagccg
      841 ccacaattaa tgcaaccaat ggagacgtta cttctgatag ccgtgattga atataccctc
      901 taaataatga ttcctctgcc agagacacaa aaaacaaatt agccaatata aactctggca
      961 accactcagg aaaatgaatc tctggcttta atccaccaaa aaaaacagcc aaaaacagga
     1021 taagaggaac agagagcgac agagcccccc acttccacaa agacacttct gattttactt
     1081 cttttttaaa caaagaagat gtacacagga ctaacaaaaa tggcaccagc gctttatcaa
     1141 aattaaaata cattgtatag ggagtacttt gagggccaac agtaacagaa tttagcacta
     1201 caggattgtg aaaccctggc catagatgga aaaataatgc tatggctgat aaaactatgc
     1261 caacttcata tatagattta gcccaggcgt tatatttcca gttgaacttc aaaataataa
     1321 aaaaaacgat tgtagcaaca aaaaataaca ccgaccaatc aataatatca ttaagcacag
     1381 ccaggacaac agacaccgtc aacaatgaaa aagccactac cttatgccaa ctgagagttg
     1441 acagtgacag tactaacaca atccacataa gcactccttt tatttaatga tgaagattga
     1501 ttatcaatat tttcaattca ccaggcaaca ttttatctac cttccacaat acatgaccat
     1561 caaaaaataa acatgttaat tataaacaca gaaaacataa ccctcatcac tatatatccc
     1621 taccgcatat ccatatctaa ccggacattc agagccataa gcattccttc aataatacct
     1681 tccgctttat aaagcctttt accaataagc ccatcagaac atctatgctt acgtgcaaga
     1741 gccataaatg tcattccacc tacgtaataa tccaccaaca aatcgtgcaa atactgatta
     1801 ttcttgttta atcgtgccat acaaccacaa attatcatgg cgtcatcatc agaacactta
     1861 ggacgtgatt tcactttagt cgggattaat cctttaaaac cagcagctat cgagggccat
     1921 gttacatctt catgattatt tgctgcccat gccccccatc gctcaagaac catctggata
     1981 tcacgcgcca tcgttaccac ctctgatttc gtaaatcttc acgcccagcc gtccacctgg
     2041 cacaggctga ccgcgcacaa tattgatttc atcaaactgc tcgtcatcaa tgagcacttc
     2101 cgcatgcgtc agcgcatcca gcggtgcttt cagaatgttg tccaggtcac ggcggcgctt
     2161 atccggtggt tctgcaataa tttttattgc cagccgtccg gacaggctta atttcagccg
     2221 ctgctggcga acaataagcg ccacagcccg gcgataacgc tcaccggctt ttgatacaaa
     2281 atatgtgctg ccacgacgtc gccagtaggt gttcaccgtc ggcgggtaag gcaaaacaaa
     2341 ctctatacgc atcagtaacc tcttttaccc gagcacgccg gttgcaaaag cgtgatcaag
     2401 aaaacgaaaa attaaatcaa cctgggaacc atgcttttct tcgaacgcca gcggatccgc
     2461 atgaagctcg ttgtgatgct cccgacacag cggtagcgtg aaaatatcgt gggattttgt
     2521 tcccattcca ccctgaccat gaccaatcag atgatgagga tcgtccgctg gcttaccaca
     2581 acacgcacac ggctgtgtct ttacccagcg tgtgtatttc tcatttaccc agcggcgacg
     2641 tttaggtcgt ttcatgaagg atttcggaga ctccggatca acggcaatgc tgaccaccgt
     2701 cttttcctgt ggtgggttct gttgctggtg ggtgtgaggc ggtagcgcaa tattttttgt
     2761 gcgctgcttc agtatgctgg tggcggtctg ttctcccggt atgatgtcgc tttcacggta
     2821 tacggagcgg attttttccg ccggtaatcc cagagaacga cgcgatactg cctcaggtaa
     2881 tgcgtccacc acctgattgc agactgccca ccaggataat tcagccagcg ataattcccg
     2941 ttcctgtatg ccattcattg cgtggcggat gacgtcaatc atccaggcgg ccagattctg
     3001 ttgagcaagt tgctcaagtg attcggatgt ctggtcgcgc agctggttgt cgcagtgcca
     3061 gcacaacacc atcgcgccgg taccgtaacg atgtatgaca gtttcgctgt gatgataatc
     3121 accatga
//
LOCUS       DAZKIF010000269         3124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZKIF010000269 DAZKIF010000000
VERSION     DAZKIF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.271"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..593)
                     /gene="dsbA"
                     /locus_tag="ACHOTW_004836"
     CDS             complement(<1..593)
                     /gene="dsbA"
                     /locus_tag="ACHOTW_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP9846762.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYA"
     gene            complement(610..1596)
                     /gene="srkA"
                     /locus_tag="ACHOTW_004837"
     CDS             complement(610..1596)
                     /gene="srkA"
                     /locus_tag="ACHOTW_004837"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP9846763.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1673..1942)
                     /locus_tag="ACHOTW_004838"
     CDS             complement(1673..1942)
                     /locus_tag="ACHOTW_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP9846764.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2012..2596
                     /gene="mobA"
                     /locus_tag="ACHOTW_004839"
     CDS             2012..2596
                     /gene="mobA"
                     /locus_tag="ACHOTW_004839"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP9846765.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2578..3105
                     /gene="mobB"
                     /locus_tag="ACHOTW_004840"
     CDS             2578..3105
                     /gene="mobB"
                     /locus_tag="ACHOTW_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP9846766.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT          817 a          762 c          758 g          787 t
ORIGIN      
        1 gcatactgct gaacaaaaac atccatattg ctggtatcca taccctgcgg attcagctga
       61 tatttaccgt taacaaacat cgccggaaca ccacgcaatt gcacgtcagc tgcagctttt
      121 tcctgctgag cgaccagaga tttcaccacg aagctgttcc acgccgcgtc gtactcttca
      181 cctttaatac ctgcgttgat aaatacatcg cggatatcag atgctgaacg aatggtctgg
      241 gttttctgta cgccttcaaa cagcggaaca gtcactttgt cttccacgcc cagcgccatc
      301 gccacagccc atgcctgagt cagatctttg cccaggtcac cacccatgaa gttgacgtgg
      361 tatttagtca tcttcacgcc ttccggcagt tttttcttca cattatcaga aatatgcaga
      421 acttcttcaa actgatagca gtgcgggcag aagaaagaga aaaactccag cacttgcggc
      481 gcgccagcta ccggtttttc cagggtagtg tactgtttac catcttcata ctgcgccgcc
      541 gatgcgctaa acgctaaaac taaaccagcc agcgccagcc aaatcttttt catgatctac
      601 tctctccgat taatacatag gtgttaattg caaagggggt tcttgtagaa cttttgcctg
      661 ttctataaaa gtcgccgtct gtcgtagcca gtaatcttcc ccggttaacc acgggaaatt
      721 tttcgggaac gcgggatcag cccaacgccg cattagccag gcaagataat aaaccaaacg
      781 catggcgcgt aaaggttcaa tcagtccgat ttcagcggtg tcgaactcgc taaattcttc
      841 ataagcttca ataatagttt ccagttgcat ccgctgctcg gctttatcgc cattgagcaa
      901 catccacaaa tcctgaatag ctggaccatt acgtgcatca tccagatcaa caaacattgg
      961 accatcgcgc cagagaatat tcccggcgtg gcagtctcca tgtagccgca gaacggtgaa
     1021 atcttcccgc cagtgtgctg taacggcggc gatcagctca tctgtcgctt tcaggaatgc
     1081 cgctttcaac ccggaaggta tcagtgtagc gtcctcaaac agcttgcgtg gctcaatgag
     1141 atattcattc agaccgatgg tcgggcgatg gataaaaagc tgtttgcgcc ccgtctggtg
     1201 catacgccct aaataacgcc caaccgcttc catctgatcg atattatcag cttcgaactg
     1261 gcgaccaccg acgcttggaa aaacagcgaa ataaaatccc tgatgattca ataaagtctg
     1321 accgttaaaa gccacaggtg ctgcaaccgg aacttcatca tttaccagct gcaacgcaaa
     1381 ttgatgttct tcgaggattt gatcggctgt ccaacgttca ggacgataaa atttgacgac
     1441 aaaacgtcga cgatcttcgt cctgaaattg atagacacgg ttttcatagc tgttaagcgg
     1501 ggtaagaccg gaatccaccc ggatcccttg ctcaaacaga gcgtccatga tggtatccgg
     1561 gtgtagtgtc tggaaagtaa aagcgctgtt gttcatccca tcatccggaa aacattacga
     1621 atgatgaagg atatcatttc acgatgattt tggtggcgcc gcttacaagc ttttactctt
     1681 taattacgcc gcgagcgcgt aacagcgccg ttttgaagtc ttcttcataa tctttctgca
     1741 aacccgggat caccgcatct ttcgcggaat cacgcatttt caggtgatag atcagaatgt
     1801 catccgtcaa atccgccagt tcgccgtcaa aacctgactc tttcgccagt ttctgcaaaa
     1861 attgcaacag gttaaggtct ggctcttttt gccaggctgg ctggaggagt tcaataactt
     1921 cattcagacg tttacatttc atagtaatgc tccttactct tgagacagac acgttagcag
     1981 ggtcaatccc acaataaaag aggcgatatc ggtgaatctg atgacgacga taacaggcgt
     2041 tgtgctggca ggcggtaaag ccagacgaat gggcggcgta gataaaggat tgctggactt
     2101 aaatggcaaa ccgctgtggc aacatgtcgc tgacgcgctt atgacgcagc tctctcacgt
     2161 cgtggttaat gctaatcgtc atcaggaaat ctatcaggca ggcggtctga aagtgattga
     2221 agattcactg gcggattacc caggccctct ggcagggatg ctttcagtaa tgcagcagga
     2281 agctggtgag tggtttttgt tttgtccgtg cgatacgcct tacattcccc atgatttagc
     2341 cgcccggctt aatcatcagc gcaaagatgc gcctgtcgtg tgggtccatg acggtgaacg
     2401 cgatcacccg actattgcta tggtaaaccg cgctattgag cctttattac tagaatatct
     2461 gcaagcagga gaacgccggg taatggcatt tatgcgtctg gctggcggtc atgcggttga
     2521 tttcagcgat cataaagatg catttgttaa cgtgaatacg ccagaggagc ttgcccgatg
     2581 gcaggaaaaa cgatgatacc gttactcgcc tttgccgcgt ggagtggtac cggaaaaact
     2641 acgcttttga aaaaactgat cccggcatta tgcgccagag ggatccgtcc agggctgatt
     2701 aagcatacgc accatgatat ggatgttgat aagccaggca aagatagcta tgagctgcgc
     2761 aaggctggcg cggcacaaac catcgttgcc agccagcaac gatgggcctt gatgacggaa
     2821 acaccagacg aagaagagct ggatctacag tttctcgcaa gtcgaatgga tacctcaaag
     2881 ctggatttga ttctggtcga agggtttaag catgaagaga tcgcaaagat tgtgctgttt
     2941 cgcgatggag ccggacatcg acctgaagaa ttagtgatag acaggcatgt tattgctgta
     3001 gccagtgatg tgccgcttaa tcttgatgtc gcattactgg atattaatga tgttgagggg
     3061 ctggctgatt ttgtggtgga gtggatgcaa aatcaggacg gataagcaag agtaggctgg
     3121 agca
//
LOCUS       DAZKIF010000270         3076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZKIF010000270 DAZKIF010000000
VERSION     DAZKIF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.272"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..478
                     /gene="agaB"
                     /locus_tag="ACHOTW_004841"
     CDS             2..478
                     /gene="agaB"
                     /locus_tag="ACHOTW_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP9846767.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            550..1320
                     /gene="agaC"
                     /locus_tag="ACHOTW_004842"
     CDS             550..1320
                     /gene="agaC"
                     /locus_tag="ACHOTW_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP9846768.1"
                     /translation="MAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQTGLITGG
                     LTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILF
                     FYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALV
                     KAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQVSNLLPVA
                     VLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1310..2101
                     /gene="agaD"
                     /locus_tag="ACHOTW_004843"
     CDS             1310..2101
                     /gene="agaD"
                     /locus_tag="ACHOTW_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP9846769.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2102..2857
                     /gene="agaI"
                     /locus_tag="ACHOTW_004844"
                     /pseudo
     CDS             2102..2857
                     /gene="agaI"
                     /locus_tag="ACHOTW_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT          766 a          716 c          754 g          840 t
ORIGIN      
        1 aatgagcagt ccaaatattc tcttaacccg tattgataac cgtctggttc acggtcaggt
       61 tggcgtgacc tggacatcca ccatcggtgc gaatctgctg gtagtcgtgg atgatgttgt
      121 cgctaacgat gatattcaac agaaattaat gggtattacc gcagaaacct acggctttgg
      181 cattcgtttc tttactatcg aaaaaaccat taacgtcatc ggcaaagctg caccgcatca
      241 gaagatcttc ctgatttgcc gtacgccaca aacggtacgt aaattggtag aaggtggcat
      301 tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg gaagggaaaa agcaaatcag
      361 cagtaaagtt tatgtcgatg accaggatct cacggactta cgttttatta aacaacgtgg
      421 cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa gaacaaatcc ctgactaaat
      481 ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc atgaaataac cctactttag
      541 ggattatccc tggcggcgtt agttttcgtt ctggggattg atttttggct ggaagcctta
      601 tttttattcc gcccgataat cgtttgtacc ctaactggcg ctattctcgg tgatattcag
      661 actggcttaa ttaccggtgg tctgacagag ttggctttcg ccggattaac ccctgcaggt
      721 ggtgttcagc cgcccaaccc gattatggcg ggtctgatga ccaccgtcat tgcatggtct
      781 acgggcgttg atgccaaaac ggcaattggt cttggcctgc cgtttagttt gttaatgcag
      841 tacgtcattc tgttcttcta ttctgctttc tcattattta tgaccaaagc cgataaatgc
      901 gcgaaagagg cggatacggc agcgttttcc cgacttaact ggacaacgat gctcatcgtc
      961 gcttcagcgt atgcggtgat tgctttcctc tgtacttacc tggcacaagg ggcgatgcag
     1021 gcgctggtga aagcgatgcc cgcttggctg acccacggct ttgaagtggc tggcggtatt
     1081 ctgcctgccg ttggttttgg cttgctgctg cgcgtgatgt tcaaagcaca atatatccct
     1141 tacctgatcg ccggcttcct gtttgtttgc tacatccagg tcagcaacct gttgccggtt
     1201 gccgtactgg gcgcaggctt tgcggtgtat gagtttttca atgcgaaatc ccggcagcaa
     1261 gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg actacagcaa tgggatctga
     1321 aatcagtaaa aaagatatca cccgtctggg ctttcgttcg tcgctgctgc aagcgagctt
     1381 taactacgaa aggatgcaag cgggcggttt tacctgggcg atgttgccga tcctgaaaaa
     1441 gatttataag gatgacaaac cgggcctaag cgcagcgatg aaagataacc tcgaatttat
     1501 taatacccac ccgaatctgg tcggattcct gatggggtta ttaatttcga tggaagaaaa
     1561 aggagaaaac cgcgacacca ttaaaggcct caaagtggca ctgtttggcc caatcgccgg
     1621 gattggcgat gcgattttct ggtttacttt gttgccgatt atggcgggaa tttgctcatc
     1681 atttgccagc cagggaaacc tgctggggcc gattctattt ttcgccgttt acctgcttat
     1741 ctttttcctg cgcgtcggct ggacccacgt cggttattca gtcggcgtga aggcgatcga
     1801 taaagtgcga gagaactcgc agatgattgc tcgctcggca accatcctcg ggatcacggt
     1861 aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg gtgacatcgt ttgccatcga
     1921 cagtacccac agcgtcgcgc tgcaacagga tttcttcgat aaagtcttcc cgaacatttt
     1981 accgatggcc tacaccctgc tgatgtatta cttcctgcgg gtgaaaaaag cgcatccggt
     2041 gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt tccgcattcg gcattttgta
     2101 aatggaacga ggcactgcgt ctggcggtgc ctctttactt aaggaatttc atcctgtgca
     2161 aacccttcag caagttgaaa actatacggc gttaagtgaa cgtgccagcg aatatttatt
     2221 ggccgtgatc cgtagcaaac cggatgccgt gatttgcctg gcgaccggag ccacgccatt
     2281 actgacgtat cattatctgg tagaaaaaat ccactagcag caggttgatg tcagccagct
     2341 caccttcgtg aagctcgacg aatgggtgga tctgccatta acgatgcctg gtacctgcga
     2401 aactttcctg caacagcata tcgtgcagcc gctggggcta cgtgaagacc agctcatcag
     2461 ctttcgctcc gaagagataa atgagacaga gtgcgaacgg gtaacgaacc tgattgcgcg
     2521 caaaggcggt ctggatttat gcgttctcgg attggggaaa aacggtcatc ttgggctgaa
     2581 cgaaccggga gaaaacctgc aaccggcctg ccatatcagt caacttgatg ccagaacaca
     2641 gcaacatgag atgttaaaaa ccgcgggtcg ccccgtgact cgtgggatca ccttaggcct
     2701 gaaggatatt ctcaatgccc gcgaggtttt gttactggtg actggcgaag gaaagcagga
     2761 tgcgacagaa cgttttctca cggctaaagt ctctacctct atcccggctt cgtttttgtg
     2821 gctgcacagt aactttattt gtttaattaa tacctaacca gatgaaaaat ttgctcaaaa
     2881 tgtttaaatt aacttatgca acagtcacgc attatattaa ataacatttg actgggttga
     2941 acataacgcc aatagcaaaa ggagttatgt tcaacaattt aattttagat atacatataa
     3001 cttgttctat taataaacca tctcgtattc tgactcgccc cctggcgcag caaagtcatg
     3061 cacttatact cttttg
//
LOCUS       DAZKIF010000271         3054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZKIF010000271 DAZKIF010000000
VERSION     DAZKIF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.273"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..1288
                     /gene="sctN"
                     /locus_tag="ACHOTW_004845"
                     /pseudo
     CDS             1..1288
                     /gene="sctN"
                     /locus_tag="ACHOTW_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
     gene            1278..1736
                     /locus_tag="ACHOTW_004846"
     CDS             1278..1736
                     /locus_tag="ACHOTW_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFP9846770.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            1714..1947
                     /locus_tag="ACHOTW_004847"
     CDS             1714..1947
                     /locus_tag="ACHOTW_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846771.1"
                     /translation="MDEVKKIEHCKILNTDTPDNSASDLDSFLKKNKKNKEMSEIVAG
                     TILQLLRQTQDNQFSTLGKKNGHMKSIDNKSSL"
     gene            2020..2589
                     /locus_tag="ACHOTW_004848"
     CDS             2020..2589
                     /locus_tag="ACHOTW_004848"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFP9846772.1"
                     /translation="MTSRDQGINSELSNRTIQFKEKIHNGIHTEYITDQKHSNNKDRE
                     KKYRDGDKINGPQAHSLDITNERRFADNRTMFTQHIEKQRNVNTLNQNDINNSANNAN
                     VRENELTYQFQRWGQNHTVRILESSEGIRLKPSDTLVSDRLHEAQHNDVTAQRWVLTE
                     QDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            2549..>3054
                     /locus_tag="ACHOTW_004849"
     CDS             2549..>3054
                     /locus_tag="ACHOTW_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HFP9846773.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPTLDKK
                     SLYWPIHFV"
BASE COUNT         1044 a          558 c          690 g          762 t
ORIGIN      
        1 aaaaattaaa attattaaat aaatacagct acttacatag cataaacggc tctctgattg
       61 aagcagaact tgacgacgtt tctgtgggtg aagtatgcga aatttatgcg agcaggcaag
      121 caaatgaacg tatagccagg gctcaggtcg ttggctttcg caatgggaag acattattaa
      181 accttatagg cagctctgtg gggcttacac gtactgcagt tttgaaacca acgggagagc
      241 agttaaccat acagatcagt gatgcctttc ttggttcggt tttgaatgcc tctggtcaaa
      301 ttatggaaag gtttgtcccg gacactccag gcgatagggg aaatttacgc ctgattgatg
      361 aactgccacc gtcgtatcag gaacgtcgag ttattaatac accacttgag acacggataa
      421 gagtcattga tggcgtactg acatgtggca ttggtcaacg tgttgggatt ttcgcctcgg
      481 ctggctgtgg caaaacggtt ttaatgcata tgctcgtaaa caatactgaa gctgatgtgt
      541 ttgttattgg cttgattggc gaacgtggta gggaagttac ggaatgcgcg gaatcgctca
      601 aaaaatcagt aaatgcagcg aaatgcgtat tggtctatgc cacctctgat ttttcgtcag
      661 tagaccggtg taatgccgcc ctgatggcga caaccgtggc agagtatttt cgagatcgag
      721 gaaagagagt tgtacttttt atcgactcaa tgacgcggta tgcacgagca ctacgtgata
      781 tgaaactggc agcgggtgaa ccacctgcca gacgtggtta tcctgcttct gtttttgaca
      841 gtttgcctcg cttactggag aggccgggac caacactaaa aggaagtatt accgagtttt
      901 ataccgtgtt actggaaggg gaagatgaat cagacccact gggtgacgaa attcgctcaa
      961 tccttgatgg ccatatctat ctaagtcgca aactcgcggg tcaaggtcac tatccggcaa
     1021 tagatgttct aaaaagtgtg agtcgagtat ttggtcaggt cactgatgaa aaacacagag
     1081 ataatgctgc acgggtaagg aagaacctca caactttgga agatctacag gtgtttattg
     1141 acttagggga atatcgcgca gggcaaatgc tgaaaatgat tttgctatga atgcccggcc
     1201 taaactcaca aattggttga aacagtcagt taatgaaaaa atgccaatga gtgaaacgct
     1261 aaaagagctt gaacgaattg ttaagtaaag ttaatcgact tatcagaaga acagcgcaga
     1321 gcctggctgc atgtgaagca tcattacaaa agttaaatgc agaaaaagaa aagttagccg
     1381 aaaaggaacg cctgtatgat atgcaactta aaaacctgca gtcactgtta gatatgaagg
     1441 agttactcgg cgaagttgta tttcgccagg atatatttta ctcgttacgt aaagttacag
     1501 tcattcagca gcagatcgct gagataaatc ttgaaaagca aaaaatagcc gagcggcgaa
     1561 aaatacttaa caaggaaata gttcaacagc aagcacaaag aaaacactgg tggctaaaag
     1621 gagaaaaata cgaccgacta aaaaagcgca tcaaaaaaca acttttaaat cagatgcttt
     1681 atcaagacga gcttgaacag gaggaaaaat ataatggacg aagtcaagaa aattgaacat
     1741 tgtaaaattt taaacacaga cacacccgat aattctgctt cggatttaga cagtttctta
     1801 aagaaaaaca aaaaaaataa agaaatgagt gagattgtcg ctggaaccat tctccagtta
     1861 ttacgtcaga ctcaggataa ccaattttct acgttgggga aaaagaatgg gcacatgaag
     1921 tctattgaca acaaatcgtc tctctaggtt gacaggaatc catctctttc tttgcaaaca
     1981 aaaggagccg agcaattttc tacaaagaaa cttctcaata tgacttcacg agatcagggt
     2041 attaattctg aactatctaa tcgaacgata caatttaagg aaaaaatcca taatggtatc
     2101 catacagaat acatcacaga tcaaaaacac agtaataata aagatcgtga aaaaaaatac
     2161 cgcgatggag acaaaataaa tggaccgcag gcccattcat tagacattac taacgaaaga
     2221 aggtttgctg ataacagaac catgtttact caacatatcg aaaagcaaag gaatgttaat
     2281 acgctcaatc agaatgatat aaataattct gctaataatg ccaatgtgcg cgaaaatgaa
     2341 ttgacatatc agtttcagcg ttggggacaa aatcatacgg tccggattct ggaaagtagt
     2401 gaagggatac gactaaaacc atcagacaca cttgtctccg acagactcca tgaagcccaa
     2461 cacaatgatg ttacagcaca acgttgggta ttgactgagc aagatgaacg acaaggacaa
     2521 cgacatcagc ctcatgagga gcaagaaaat gaaggcaaat tcgaaaacga tcagaaggat
     2581 gaaagttaat gttcggtctg aggaaagtaa atcgaaacac tctacgtttg aaaccacttt
     2641 tcaaaattgg aaagaaaatg gcgaagatgt tgcattatta atgcccgaat ttagtgctaa
     2701 atggctaccg atagcagaag agagtggcag ctggtctgga tgggtgttgc ttcgagaaat
     2761 atttccctta atttctgccg agctcgctgg gatggcttta atgccagaaa cggaaaggtt
     2821 aataggggaa tggctcagtt tatccagttc cccgttaaat ctaaagtatc ctgaactaaa
     2881 atataatcgt ttgtgcgtag gtaaagtatt tgatggagta ttgagtccag ctcaaccact
     2941 gataagaata tggacagggg aattgaatct ttggttagat aaagtcacag tctgccaata
     3001 cgaaaacgct ccaactttag acaaaaaatc attatattgg cccattcatt ttgt
//
LOCUS       DAZKIF010000272         3054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZKIF010000272 DAZKIF010000000
VERSION     DAZKIF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.274"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(43..162)
                     /locus_tag="ACHOTW_004850"
                     /pseudo
     CDS             complement(43..162)
                     /locus_tag="ACHOTW_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            913..1119
                     /locus_tag="ACHOTW_004851"
     CDS             913..1119
                     /locus_tag="ACHOTW_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9846774.1"
                     /translation="MEKITTGVSYTTSAVGTEYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(1375..1647)
                     /locus_tag="ACHOTW_004852"
     CDS             complement(1375..1647)
                     /locus_tag="ACHOTW_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846775.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1807..2340
                     /gene="rrrQ"
                     /locus_tag="ACHOTW_004853"
     CDS             1807..2340
                     /gene="rrrQ"
                     /locus_tag="ACHOTW_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9846776.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            2561..2674
                     /locus_tag="ACHOTW_004854"
     CDS             2561..2674
                     /locus_tag="ACHOTW_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846777.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            2676..2993
                     /locus_tag="ACHOTW_004855"
                     /pseudo
     CDS             2676..2993
                     /locus_tag="ACHOTW_004855"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2938..>3054
                     /locus_tag="ACHOTW_004856"
     CDS             2938..>3054
                     /locus_tag="ACHOTW_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9846778.1"
                     /translation="MVVSACGSTPPAPVPCVKPPAPPAWIMQPAPDWQTPLNG"
BASE COUNT          816 a          655 c          785 g          798 t
ORIGIN      
        1 gtttttactt ctcctcagat acgatatgta aaaatcgcgc taggcatcca gatccagttg
       61 ccctcgctta ttcgatatcg gggcacccgg caatttctgc aatctgcgaa gctggcgatc
      121 ggtcagactt aaatgcctgg caacttcagt ctgcgtagcc actcctcacc tcgcaaaaac
      181 tctcacctca caatcacaac aaaaccggtc atgtccggtt tacatgtctg ttttttgttc
      241 atgtccggtt cacagaagac ctgtttttat atttttcata tagttaactt gaagagaaac
      301 cggacatgga tcccggaaaa ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt
      361 aacctgtcgg atcgccggaa aggacccgca aaatgataat aattatcatc tacatgtcac
      421 aacgtgcatc tacgccatca aaccacgtca aataattaat tatgacgcag gtatcgtatt
      481 aattgatctg catcaactta acgtaaaaac aacttcagac aatacaaatc agcgacactg
      541 aatacggggc aacctcatgt caacgaagaa cagaacccgc agaacaacaa ccagttacag
      601 atgctttatt aaggaaaaaa acagccagca ctgactttcg gtggagaggt gctggctcag
      661 aaggatagtt ggatttcaca tgatacttat gcctggcggt atattttctg acagacagtg
      721 acgggtgttg tcaagatatt gtgtcattta taacctgaat caggggaggc cggaatgtta
      781 tctggcattt ttagcagagc ctgaatgcca taatcacggc tcccggagtt ggccgtcagt
      841 gggtgacact ggcggctttt ttgtttttct ttactttcat tttctgtcgg cggtgacgga
      901 gacatacatc agatggaaaa aatcacaaca ggtgtgtcat acaccacgtc agcggtgggg
      961 acggaatact ggttactgca gctgctggac aaagtctctc cgtcccagtg ggtggcaata
     1021 ggagtgctgg gaagtctgct gtttggcctg ctgacgtatc tgactaacct gtatttcaaa
     1081 atcagagagg accgtcgtaa ggtggcgcgg ggagagtagt cgatgaataa acaatacgaa
     1141 ctggttgtaa aatgaatatt tctaactgaa aaaacgttcc atgaggtaag aaaaggtcac
     1201 aggcaatcaa taacaggacg tgatgaaaga cccttgcatt tgtgcgcttt ctctttagat
     1261 agcagcagat actgaaaatc tgagttgtcg gggagtcagg gatacagctg tgcaagagtt
     1321 ggtcattgtg attccattga aatcctgtat gccatgaagg gcaggatttt atggctacct
     1381 gagctttggt gatagtaagt tgaaaattcg cattttttgc tgacatgcgt aacgagaatc
     1441 ccataagcag ggaggactta attcttcatt aacccatgcg ttgatattat gtttcagccg
     1501 ttgaagcatc agcggtgtta atgttgtggt aataatatcc agcgttttat gtgagatctt
     1561 accgtaaggg tctgcaagaa tgctgcttgt tgcttcgtta ttatctgcca tcagaagaag
     1621 taactctgat ttaacgtttt ctgtcattag ttgtaaaaat cttctgcgca aactttcttt
     1681 actgttcatt tatatggctt catttgttgt aatctgctgc gtctcaaggg atatgtttat
     1741 gagagcgacc atgagtgttg gattatatac ctaacatatc aagggattag aaatcgataa
     1801 atccccatga acgaaaaaat aaaatacggc ctgtcggctg ccgttctggc gctgattggt
     1861 gcaggtgctt ctgcgcctga aatcctcgac cagtttctgg atgaaaagga aggtaaccac
     1921 accacggcat accgtgatgg tgcggggatc tggaccatct gccgtggtgc cattctggtg
     1981 gatggtaagc ctgttattcc tggcatgaag ctgtcaaagg aaaaatgcga ccgggttaat
     2041 gccatcgaac gtgacaaggc gctggcatgg gtggagaaaa acatccgggt gccgctgacc
     2101 gaaccccaga aagcggggat cgcgtcattc tgtccgtaca acattggccc cggtaagtgc
     2161 ttcccgtcga cgttttataa acgaattaat gcaggcgatc gaaaaggtgc ctgtgaggcg
     2221 attcgctggt ggattaagga cggtggcaga gactgccgta tccgttcaaa taattgctac
     2281 ggtcaggtct cacggcgtga ccaggagagc gcgctggcgt gctgggacat cgacagatag
     2341 cagaatattt tcctgaaaaa tgacgttggc caacgcgggt ggataacacg aaatcctgaa
     2401 aactggtaaa acctaagtga ataaaagtaa aaaccccgtt tgttggcagc aagcggggtt
     2461 ttgtgttttc tgaccttgag taaggcaagg gagaaattat gggtagggag gtactttccc
     2521 tgtgaggaag tataaaagat tctttctgag gttgtccatt atgaaaggca ttgaagtgga
     2581 gacgccagcc agtctggatt taacaagagc ggcagctttt gccattcgta ttgtggccat
     2641 tgctgttctg gtctgggcaa tccgttggtg gtgatatgaa ccgtgttctg tgtgtggtta
     2701 tcattgtcct gctggtggcc tgtggtgcgc ttagtctggg gctgaatcat taccgtgatc
     2761 acgccatcat ctacaaagag cagcgcgata aaaaagccag tgagctggag ctggcgaacg
     2821 cgacaattac tgatatgcag atacgccagc gtgatgtcgc tgcacttgat gccagatact
     2881 cgagggaatt agccgatgcg agagctgaaa atgaaactct gcgtgctgat gttgccgctg
     2941 gtcgtaagcg cctgcggatc aacgccacct gctccggtac cgtgcgtgaa gccaccggca
     3001 cctccggcgt ggataatgca accggccccc gactggcaga caccgctgaa cggg
//
LOCUS       DAZKIF010000273         3048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZKIF010000273 DAZKIF010000000
VERSION     DAZKIF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.275"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(282..1760)
                     /locus_tag="ACHOTW_004857"
     CDS             complement(282..1760)
                     /locus_tag="ACHOTW_004857"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP9846779.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLSTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(1787..>3048)
                     /locus_tag="ACHOTW_004858"
     CDS             complement(1787..>3048)
                     /locus_tag="ACHOTW_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP9846780.1"
                     /translation="RRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSNTEIGLI
                     MSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVMLCIQVAF
                     AITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFSRFAPDD
                     STSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVLRISTTW
                     YCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPLGGIITT
                     YSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYASRGLYWA
                     CPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWLMGMAAL
                     AMVIVFTFLLFQKIRTADSAPAMASSK"
BASE COUNT          805 a          791 c          691 g          761 t
ORIGIN      
        1 gttcttatga ataaagatat ccagttcata ctttaagtga aaattgataa agtgcgattc
       61 gtattgtctt ttattctaaa gacatcgagt gtagttaata ttccttgtaa aaacagggat
      121 aaaccgaact agttaaagtt tttattatgt tccctgcatc agcggggata aaccggactt
      181 gttatcattt tttaagttgt cgaacttatg ttccccgcgc cagcggggat aaccattcag
      241 gtatgttcag gccgggcagt ttcccgcccg gtcttcctca cttacgcatt cgaccagctc
      301 tggatcgaat ccgcattccc caattcccca tgcatcactt catacgattt tttcaggatc
      361 acatcagtat gtttggttag ggctttgaaa atacctttat tcatcgcgtc gtagcgtttg
      421 gcattgctct caatcggtat aaagatatct ttcacgcgaa ccattttgtc gacagccgtt
      481 gcgtaatccg ggtatagccc cagacctacc gccgtattga tcgctgcccc cagacttgca
      541 caaccgttaa tggcgttacg tcgtgccgga aggttgaaca cgtcggcaaa aatctgcata
      601 aacagatcgc tgttcgaacc gccaccagta atgatcacat gcttcgcaaa gtgattcatt
      661 tcattacaca tattgtcgta attgttcttc agcgtcagcg ccacgctttc cagtatcgaa
      721 cgatatatcc atgcgtaatc catgctggaa tcaaagccga tcataatccc gcgtttgtac
      781 ggttcccacg gattggtcag ccagtccagc accgtcatca gcccattaca gccaggtggc
      841 acacaagaag cttttttgtt gagtaaatct tccggtgaaa gatcctgcgc cctggcatcc
      901 tgaattaacg actcgcctaa catgtcgcgc agccagctca ccgtccacat gcctttgcga
      961 ataccgtaac cttcatacag caatgtttgc ggaatagaag acataatcgg ccagtacgcc
     1021 accggatctt tcggcaaggc tttgccgttc atcatcagcg cgatataagt actgagtgaa
     1081 atcaccgccg tttcatcatc cagtaatccg gctcccagag cttctaccgg tttatcactg
     1141 gtggtacaaa caaccggcag ccctgccggg aaatgtgtcg ccagcgcggc ttgtggtgtg
     1201 atctgtccaa gaacagtgcc tggcatttgc acatcaaaca gcatatgacg ggggatatta
     1261 aacttctcca ttaccgcagc atcttcgctc catgcccagc tcttataatc caccggccac
     1321 tgaccaaaat agttggcgat attgtcttta aactcgccgg ttaagcgatg cgtcagataa
     1381 cccgaaaaag aggtgacata ggccacgtca ggattagtgt gttcgtaggg acgcgttacg
     1441 cgtgcatcct gccagctaat caacggcgca gcaggcgtgc catcggcttt caataacgca
     1501 cggcagcaac ggatggatcc cagaccaata ccgacaatat cttctttatt cccggcaaac
     1561 tgactcatca aatcgtgacc ggcaaaacat aatgatgccc ataaatcgtc gtcaggatgt
     1621 tctgcggtat cggcatctgg cgtgtgcatc ggctgcaata aacctttgcc ttcgcaaacc
     1681 acgttacctt ccagatcgta catcaccact tttgtgctct gacttccgcc atcaatccct
     1741 atgatgtatt tcttcgacat gatttttctc ctgtaatttt cctggcttac ttgctgctgg
     1801 ccattgcggg ggcgctatca gcagtacgaa ttttttggaa cagcaaaaag gtaaagacaa
     1861 tcaccatggc aagcgccgcc atgcccatca gccacatatt gcggtaggct tctgctgcgg
     1921 gctgggtgtc ttgccagtgg ccgataattg ggtaaacgaa gacatccggc aggaatccaa
     1981 tcaccgagca aatacctacc gtggtgccca taatgtaaga cggtgttctc gcttcgccag
     2041 ggcaggccca gtacagcccg cgtgaggcgt aacaggtgaa tcccagcagt aaaatcaggc
     2101 caatccccat cgcgaccgat tgcgggttag agttcgtgac gagcagggca gttaacgcca
     2161 gcaggccgag tacggaaagg atttggatca cgcgggtagg ggatttcact ttactgtagg
     2221 tggtgataat gccgccaagt gggccgcaca gcgcgcggaa gattttgttg atcacaatcc
     2281 ccatgtagct cgccgccacc agcgacatgc catacatttc ggtcagatag ttggtggagt
     2341 aactcagaat ggcgtagatg gtgaagacgc caaaaatcac catgctgcaa taccaggtgg
     2401 tgctgatacg caaaacggca aggatgtcgc taagctggaa tgactgtttt tcttcgttat
     2461 tggcactgcg caggttgttg ttatcgctaa caaaaaacca gcacagaatc cccaacaaga
     2521 tgtaaaccac actatagatg ataatgaccg ttttcaggct ggtgctgtca tccggtgcaa
     2581 agcgagaaaa aacccacatg gtaaacactg ccagcgacat tacaccgacg ccgcgcagcc
     2641 cttccatcca gcccataatt ttcccttgct cgctatgatc gccaagcaac gatgcggctt
     2701 taatcgacac cgaccacagc attaaaatcg ttgttatcgc aaaggcgacc tgaatacaga
     2761 gcattaccca cagcggtgga tacgttgcca ttaacagacc cagtaatccg gtaatgatca
     2821 tcgcggaagt aatcattttg cggtgtgaaa atttatcggc aataacgccg ctgggggcat
     2881 aaagaataat agccgcaata ccaaaggtac tcattattaa acctatctcg gtattgctga
     2941 atcccataaa tttcgccatg ggaatttgat aaatataacg taaataagcc aggtcgaaac
     3001 taacgccgcc gctaaaacta attatcgcga gggttatcca acggcgat
//
LOCUS       DAZKIF010000274         2972 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZKIF010000274 DAZKIF010000000
VERSION     DAZKIF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.276"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..331
                     /locus_tag="ACHOTW_004859"
     CDS             <1..331
                     /locus_tag="ACHOTW_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9846781.1"
                     /translation="PEDEGKNALKFAMQNRIKLFYRPAGIAPVNKTTFKKLRVSRSGN
                     NVVIKNDSPNWVTISDVKANNVKVNYETIMIAPQESQNVSEKNNNANGWQLTIIDDHG
                     NYISDKI"
     gene            347..2827
                     /gene="yehB"
                     /locus_tag="ACHOTW_004860"
     CDS             347..2827
                     /gene="yehB"
                     /locus_tag="ACHOTW_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9846782.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNTLYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
     gene            2838..>2972
                     /locus_tag="ACHOTW_004861"
     CDS             2838..>2972
                     /locus_tag="ACHOTW_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFP9846783.1"
                     /translation="MMFRNRILLIFILWANFTWAGCRTTASLNITDGINVGEILANET
                     S"
BASE COUNT          866 a          549 c          739 g          818 t
ORIGIN      
        1 cccagaagat gaaggaaaga atgcgttaaa atttgccatg caaaacagaa tcaagttgtt
       61 ttaccgacct gctggtattg ctccggtaaa taaaacgact tttaaaaaat tacgagtgag
      121 tcgtagcggt aataacgtag tcataaaaaa tgactcgcca aattgggtaa cgatttctga
      181 tgttaaggct aataacgtca aagtgaatta tgaaacaatc atgattgcac cacaagaaag
      241 tcagaatgtt agtgaaaaaa ataataatgc gaatggctgg caactgacca ttatcgatga
      301 tcacggcaac tatattagtg acaaaattta attgcaggag ctgcctatgt tgagaatgac
      361 cccgcttgca tcagcaatag tcgcgttatt gctcggcatt gaagcacatg cagcagaaga
      421 aacctttgac acccatttta tgatgggagg aatgaaaggt gagcaagtaa caaatttgcg
      481 tcttgatgat aatcagcctt tacctgggca gtacgacatt gatatttatg tcaataaaca
      541 atggcgtgga aaatatgaga ttatcgtcaa agataaccca catgaaactt gcctgacgcg
      601 tgaaattgta aagcggttag gtataaatag cgataatttt gcacgcgaaa atcagtgttt
      661 aacatttgag caacttgttc agggcggaag ctactcctgg gatattggga tttttcgttt
      721 ggatctcgct gttccgcaag cctgggttga agagctggaa aatggttacg ttccgccaga
      781 aaactgggag cggggaatca atgcgtttta tacgtcttat tacgtaagtc agtattacag
      841 tgactataaa gcctcgggca atagtaagag cacttatgtg cgttttaaca gtgggctaaa
      901 tttgctggga tggcagctgc attcagatgc cagttttagc aaaacggata ataatcctgg
      961 tgagtggaaa agcaacacgc tgtatctgga gcatggattt tctcaaattc tcggtacgct
     1021 gcgtattggt gatatgtaca cctcagccga tatatttgat tccgttcgct tcaccggggt
     1081 tcggttattt cgtgatatgc agatgttgcc aaactcgaag caaaatttta ccccacgggt
     1141 acaggggatt gcccagagta acgcgttggt aactattgaa cagaacggtt ttgtcgttta
     1201 tcagaaagag gttccaccag gtccgttttc gattagtgat ttgcagttag cgggcggagg
     1261 ggcggatctt gatgttagtg ttaaggaagc cgatggttct gtgactacgt atctggtgcc
     1321 ttatgcggca gtacctaata tgctgcaacc tggtgtatca aaatatgatt ttgcggcagg
     1381 ccgtagtcat attgaaggtg cgagcaagca aagtgatttt gtccaggcgg gttatcagta
     1441 tggttttaat aatttattga cgctgtatgg cggcacgatg gttgctaata attactatgc
     1501 ctttaccctc ggaacaggtt ggaacacgcg tattggcgca atttcagtcg atgcaactaa
     1561 atcgcacagt aagcaagaca atggtgatgt gtttgacgga caaagttatc aaattgccta
     1621 taacaaattt ttgagccaaa catcaacacg ttttggtctg gcggcctggc gttattcgtc
     1681 gcgtgattac cgcacattta acgattatgt gtgggcaaat aataaagata attatcgtcg
     1741 tgataaaaac gatgtctatg atattgccga ttattaccag aatgattttg gtcgcaaaaa
     1801 tagcttttcc gccaatatga gtcagtcatt gccagaaggc tggggatctg tgtcattaag
     1861 tactttatgg cgtgattact gggggcgaag cggcagtagt aaagattatc aactgagtta
     1921 ttccaataac tggcggcgga taagctatac cctcgcggca agccaggcgt atgacgagaa
     1981 ccacgccgaa gagaaacgct ttaatatttt tatatcgatt ccctttgact ggggcgatga
     2041 cgttacgacg ccgcgacggc aaatatatat gtctaactca acgacctttg atgatcaggg
     2101 ttttgcttca aacaataccg gattatcggg aaccgttggg aaccgcgatc agtttaacta
     2161 tggtatcaac ttgagccatc aacatcaggg aaatgaaacg acagctgggg ccaatttgac
     2221 ctggaccgca ccggccgcaa cagtgaatgg cagctatagt cagtcgagta cttatcgaca
     2281 ggtcggagcc agtgtttcag ggggactggt tgcctggtct ggtggcgtta atctggcgaa
     2341 ccgtctttcc gaaacgtttg ctgtgatgca tgcgccggga atcaaagatg cttatgtcaa
     2401 tgggcaaaaa tatcgtacaa caaactgtaa tggtgtggtg gtgtacgacg gactgacacc
     2461 ttatcgggaa aatcacctga tgatggatgt gtcgcaaagc gatagcgaaa cagaattacg
     2521 cgggaaccgt aaaatgaccg ccccttatcg cggcgcggtc gttctggttg attttgatac
     2581 cgatcagcgt aagccctggt ttataaaagc attaagatcc gatggacagc cattaacgtt
     2641 tggttatgaa gtcaatgata tgcatggtca taacattggt gttgtcggcc agggcagtca
     2701 gatatttatt cgcaccaatg aaataccgcc agcggttaat gtggcaattg ataagcaaca
     2761 agggctttca tgcacaatca ccttcggtaa agagattgat gaaagtaaaa attatatctg
     2821 tcggtgagag gtgaaatatg atgttcagaa atagaatatt actaatattt atattgtggg
     2881 ctaattttac ctgggctggg tgtcgtacta ctgcatcatt aaatattaca gatggtatta
     2941 atgttgggga gattttagcg aatgaaactt cc
//
LOCUS       DAZKIF010000275         2935 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZKIF010000275 DAZKIF010000000
VERSION     DAZKIF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.277"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(366..2714)
                     /gene="nleL"
                     /locus_tag="ACHOTW_004862"
     CDS             complement(366..2714)
                     /gene="nleL"
                     /locus_tag="ACHOTW_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFP9846784.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISL
                     DELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMEN
                     GSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVT
                     QQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWM
                     SFFLYKDGEVQNTFGYSLSNLFSESFPIFSIPYHKAFSQNFVSGILDILISDNELKER
                     FIEALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQ
                     AEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDI
                     LDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
     gene            complement(2734..2823)
                     /locus_tag="ACHOTW_004863"
     CDS             complement(2734..2823)
                     /locus_tag="ACHOTW_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846785.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            complement(2836..>2935)
                     /locus_tag="ACHOTW_004864"
     CDS             complement(2836..>2935)
                     /locus_tag="ACHOTW_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9846786.1"
                     /translation="RIPTASQNRSPAPVFVVMSGEYLLDKKEEIIQ"
BASE COUNT         1016 a          614 c          425 g          880 t
ORIGIN      
        1 acaaaatcaa ttatcacatt caaaaaaata ctgacaacaa agatactctt cagacaaagt
       61 tgtcatatgt aaattttccc ttaaaacgtt gctcaagaaa aatataaaaa tcatatgccc
      121 taatcaacat cctcaagcac tacgatatat gcagtatcct attcatatat taccaccata
      181 tcagacaaat cgaatcacaa cgagttctat cgtactcttt catcttcgac actgtaaatt
      241 ttaagaacat tcggctcatg ccactaaacc aagacataat aacaacggag caataaatgt
      301 tcccactgaa catgtgcaaa cctaaaaaga cagtgttaca ataatattca tccatggtct
      361 ctaaatcaac gccacgcaac aggataatac aatgaaaata tttcaggaaa agattctttt
      421 gcatgctccg ttagcatatc agtcagcaca gcggtacaag taaaaacatt atttcttcct
      481 tgtagcctgt ctaaaatatc attgtaataa taaactgagc caaatacctg aggatctgtt
      541 ttataagctt gttcaatcaa tccatttgca tatctccgta gaatctcagg agaatcatac
      601 tctgtaccaa acatatcact cgatgagtat ttacagaaaa ccccccctaa acaaaataat
      661 atttcagcct gttttcttag tggcatatct ttcaatacat ggctccccat aatcatatct
      721 acatgctgag cgttcagttc ccaaccatca agaaagggat tccagacaca ggcaagtttc
      781 ctttgctgat catcagcaat cattttataa tctgatttat tggaattaag tgcctcaata
      841 aatctctctt tgagttcatt atcagaaatg agtatatcca gaataccaga aacgaaattc
      901 tgggaaaaag ctttatgata aggaatactg aaaattggaa atgattcaga aaaaagattg
      961 ctcaatgaat acccaaatgt attttgaact tctccatcct tatataaaaa aaaggacatc
     1021 caattagttg atgtattagg agataacata tcaaccatat gactcaaaga acacatcatt
     1081 gccataccat catcctgtga tgataacaaa acccagttat aagccaaatc ggaatcatca
     1141 tcccagtcag gcttcccaga gccatcgcac aatccaaaat cactctgctg tgtgacagga
     1201 taaacttttt cattttttaa atatacttca tagagttccc taaatcgttc ctgcattcca
     1261 tcacgccgtg aaccattcat tacaatttgt ataaaggaac tattataaga taccatcgca
     1321 cctggatgtc gctcaaaata catccctgcc tgcaataaac taccattctc catatacatc
     1381 attattgaat tattaaacca agacattata gtatcaaaaa agtcatcaca tataggctcg
     1441 agccattgct gtatatttgc gttgttcaaa taaggatgaa tactaaatga atcagccaaa
     1501 gacatcttaa catagggccg gaacgccgct aattcgtcaa gagatatttt tttgataagt
     1561 tcttcagcgg cccttacttt atcatggtta tacttatcat cagatgtatt catagtcaat
     1621 aaaattgatt tatttgattt tgtatagtta tccaacacag ataatatatt ttcctgtatg
     1681 tcagatagat ctatgtaaca ccctcctaga ttaatggtat tacttttttc actaaagagc
     1741 tcctttacat cacctaagat agcaccactt aaaaccattc caggaattat aatagctcct
     1801 gttaaatcag cctcgttcaa aattggagga tattgtatat atgaagatcc tttaaatgat
     1861 gcatttttaa gattggaatt gctgaaatta gagttttcaa acaaagccct aataatacaa
     1921 ttagataaat tagcataaca aaaattacat tcattcaaaa tcgagttttt aaaagaacag
     1981 ttttccaata cagcctgaga caaatcactc atcctgagat ctgtatcatc aagcactgct
     2041 ccagaaaaat ttactgatga taaattcagc cctacaagac ttagacctga caagtcacat
     2101 ccagagtaat tcaattcttc agcagactct tcacccgttc ggttagctga caaccaaatt
     2161 aagtcagcag taagctcagc tttgctcagg caggcacggc cttgactgac atcatatgca
     2221 aggaattgac attgcgattc atttaatgaa ttacagccat ttaaatatac ggaatttctg
     2281 agtgcctcag gaaaggaacc ttctatatgt ttcaaagaac tacaatggca taaagataaa
     2341 ttaatgacat tttcaggtat gctgcaattt ataacctcta atgatgagca tccaaccata
     2401 cttaatgaag ataagttggg gggtaagcag tttattgatt taagctctgt acatccattc
     2461 aaaaccagtt ctttcagaga acctggaagc aagtctggta atgttgttat tggctcactg
     2521 attgataaag tctctccatt agtacttata acatcaagta tttttgatgc aacctcatga
     2581 cgattttcgc taaattctcc ctcagcacac cacttttcga gggcaacttc aacatcctcg
     2641 ttagatggtg aatctacagg actttcaaaa gatattactc cagaatttac agagatattt
     2701 gtagtgggca gcatttatct cactcctttt tacttagtcc actaaagtaa aattagaaaa
     2761 cacaactgcc gggacaactt tctgtccctg tacccacaaa taagatgtca gatttatcgg
     2821 cattaattta actccctatt gaattatctc ctctttttta tctaacaaat attccccgga
     2881 catgacaaca aaaaccggag ccggactccg gttttgtgaa gctgtcggaa tacgt
//
LOCUS       DAZKIF010000276         2912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZKIF010000276 DAZKIF010000000
VERSION     DAZKIF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.278"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            23..1228
                     /gene="yedE"
                     /locus_tag="ACHOTW_004865"
     CDS             23..1228
                     /gene="yedE"
                     /locus_tag="ACHOTW_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP9846787.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            1225..1458
                     /gene="yedF"
                     /locus_tag="ACHOTW_004866"
     CDS             1225..1458
                     /gene="yedF"
                     /locus_tag="ACHOTW_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP9846788.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1567..2235
                     /gene="yedK"
                     /locus_tag="ACHOTW_004867"
     CDS             1567..2235
                     /gene="yedK"
                     /locus_tag="ACHOTW_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP9846789.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            2346..2825
                     /gene="yedL"
                     /locus_tag="ACHOTW_004868"
     CDS             2346..2825
                     /gene="yedL"
                     /locus_tag="ACHOTW_004868"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9846790.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT          653 a          726 c          770 g          763 t
ORIGIN      
        1 tctgagttca gaggctacat tcatgtcatg gcagcaattc aaacacgcct ggttgattaa
       61 attctgggcg cccatccccg cggtcatcgc ggcgggtatt ctctccactt actattttgg
      121 catcactggc accttttggg ctgtcacggg tgaatttacc cgttggggcg gtcagctcct
      181 gcagctgttc ggcgtccatg ctgaagagtg gggttatttt aaaattatcc atctggaagg
      241 atcgccatta acccgcatcg acgggatgat gatcctcggt atgtttggcg gctgctttgc
      301 cgcagcgctg tgggccaaca atgtcaaact acgcatgccg cgtagccgta tccgcattat
      361 gcaggccatt attggcggca ttatcgccgg ttttggcgcg cgtctggcaa tgggctgtaa
      421 cctggcggca ttctttaccg gtattcctca gttctcgctg catgcctggt tctttgccat
      481 cgccactgcc attggttcat ggtttggcgc gcgctttacc ctgctgccca tcttccgtat
      541 tcccgtgaaa atgcagaaag ttgctgccgc ctcaccgctg acgcaaaaac cggatcaggc
      601 gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggc atgctgggct gggcgctgct
      661 cacagcgatg aaccaaccca aactggggct ggcaatgctg tttggcgtcg gctttggttt
      721 actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt ggatcaccgg
      781 acgtacccat atggcgaaag caatcattat tggtatggcg gtgagtgcca tcgggatctt
      841 cagttacgta cagttaggcg ttgaacccaa aatcatgtgg gcgggaccaa acgcggtaat
      901 tggtggttta ctgtttggtt ttggcatcgt gctggctggc ggctgcgaaa ccggctggat
      961 gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca atgtgatcgg
     1021 ctcaacgatt ctggcgtatt actgggatga tttcgctccg gcgctggcca ccgactggga
     1081 caaaatcaac ttgctgaaaa cctttggtcc gatggggggc ctgctggtga catatttgct
     1141 gttgtttgct gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc gccgtgcggc
     1201 accgcagact gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg ccttgatatg
     1261 gtgggtgaac cctgccctta tccggcagtc gcaacccttg aggcgatgcc gcagttaaaa
     1321 aaaggggaaa tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa tattccactg
     1381 gatgcgcgta atcacggcta tacggtgctg gatattcagc aagacgggcc gaccattcgt
     1441 tatttaattc agaagtaatc cctcattccc gctgtgaccc acagcgggaa catcgttacc
     1501 tttgtatcac atagatttta cccgccccct ctcctattct tttcagtatg atctcaggag
     1561 gtttctatgt gtggacgctt tgcccaatcc caaacgcgtg aagattacct tgcgcttctc
     1621 gcggaagata ttgaacgcga tattccctat gatcccgaac ccattggcag atacaacgtc
     1681 gcgccgggaa ccaaagtcct actgctcagt gaacgtgatg aacaccttca tctggatccg
     1741 gttttctggg gatatgctcc cggatggtgg gataaaccgc cgctgattaa cgcccgcgta
     1801 gaaactgcgg caaccagtcg tatgtttaaa ccgctctggc aacatggtcg ggcaatctgt
     1861 tttgccgatg gctggtttga gtggaaaaaa gaaggcgaca aaaagcagcc ttattttatc
     1921 tatcgtgctg atggacaacc tgtttttatg gcagcgatag gcagcacacc gttcgagcgc
     1981 ggtgacgaag ccgaaggatt tttgattgtc actgcagcag cagaccaggg tctggtagat
     2041 attcatgacc gccgcccgct ggttctgtcg ccagaagctg cgcgggaatg gatgcggcaa
     2101 gagattagcg gtaaagaagc ctcagaaatc gcggccagtg gctgtgttcc agcaaaccag
     2161 ttctcctggc acccggtatc gcgcgcggtg ggtaatgtta aaaaccaggg tgcggagtta
     2221 attcaacctg tttgataagc ctggagatta ttgatcaaca atactgcgtc ataagtaatc
     2281 tctattagac aaagatttca ttacctgttg gcatattgca aaaataacac caatacggaa
     2341 tcgtcatgtt cacgattaaa acagatgatc tcacccatcc agcagtgcaa gcattagtgg
     2401 cttaccatat ttccggcatg ctgcagcagt ctccccctga aagcagtcat gctttagacg
     2461 tgcaaaaatt acgtaacccg acagtgacat tctggtcagt atgggaaggc gaacaactcg
     2521 caggaattgg tgcgctgaag ttgctggatg ataaacatgg cgaactgaaa tcaatgcgga
     2581 ccgcgccaaa ttatttacgt cggggtgtcg ccagtctgat tttacgccac attttgcagg
     2641 tcgcccatga cagatgcctt catcgcctga gtttagaaac gggtacacag gctggattta
     2701 cggcctgcca tcaactttat ttgaagcatg gtttcgttga ttgcgaaccg tttgccgatt
     2761 atcaacttga tccacacagt cgatttttgt cattgacgct atgcgaagat aatgagttgc
     2821 tttgagccag acgcagcaca ttcttgcatt cgacgtgctg cgtctttatt tatcaccaac
     2881 aggaaacgcc ttgtccatag acgccccttc ca
//
LOCUS       DAZKIF010000277         2899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZKIF010000277 DAZKIF010000000
VERSION     DAZKIF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.279"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..183
                     /locus_tag="ACHOTW_004869"
     CDS             <1..183
                     /locus_tag="ACHOTW_004869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846791.1"
                     /translation="WRLAVVRCGWQQTITVTTRSPTKRSAIKKPESWNRRMPPLLTCR
                     CASVMLLRSMQNTRGN"
     gene            187..393
                     /locus_tag="ACHOTW_004870"
     CDS             187..393
                     /locus_tag="ACHOTW_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9846792.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            458..682
                     /locus_tag="ACHOTW_004871"
     CDS             458..682
                     /locus_tag="ACHOTW_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846793.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            1039..1179
                     /locus_tag="ACHOTW_004872"
     CDS             1039..1179
                     /locus_tag="ACHOTW_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846794.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(1309..1494)
                     /locus_tag="ACHOTW_004873"
     CDS             complement(1309..1494)
                     /locus_tag="ACHOTW_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9846795.1"
                     /translation="MSSKNRPRRTTIRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            1536..1901
                     /locus_tag="ACHOTW_004874"
     CDS             1536..1901
                     /locus_tag="ACHOTW_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9846796.1"
                     /translation="MAVLRTLPGRIKTLNTRRINVLRGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAASGMPD
                     PTLPELPDGTLRADGITGL"
     gene            2191..2754
                     /locus_tag="ACHOTW_004875"
     CDS             2191..2754
                     /locus_tag="ACHOTW_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9846797.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            2751..>2899
                     /locus_tag="ACHOTW_004876"
     CDS             2751..>2899
                     /locus_tag="ACHOTW_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846798.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPG"
BASE COUNT          700 a          668 c          804 g          727 t
ORIGIN      
        1 tggcggttgg ctgtggtgcg ctgtggctgg caacaaacca ttaccgtgac aacgcgctca
       61 cctacaaagc gcagcgcgat aaaaaagcca gagagctgga acaggcgaat gccaccatta
      121 ctgacatgca ggtgcgccag cgtgatgttg ctgcgctcga tgcaaaatac tcgagggaat
      181 tagccgatgc gagagctgaa aatgaaactc tgcgtgctga tgttgccgct ggtcgtaagc
      241 gcctgcggat caacgccacc tgccccggta ccgtgcgtga agccaccggc acctccggcg
      301 tgggcaatga tgccgccgtc gaactctctc cggttgctgg acgaaacgtt ctcggtatca
      361 gagacggaat catcagcgac caggcagcat tgagaatgct tcaggaatat atccgcactc
      421 agtgtattaa ctagtatttt tgttattcgg agaatgcatg aagaaattat tggtaaccgt
      481 aaagcctttt cagggaacaa ttccgttccg tattttgcag cgtggtcgtg ttcttgttga
      541 aggttcgttc agtggtaaat gtacgcaatt acactccagg acctttcagg tgaatgccac
      601 gaatgaagag ctaaccgttg agtgtacgat gaatgccgct aaatgccgca tggtatccgc
      661 tgcattacag ccagtgtgtt gagcgacctt attattcatg cgcggtattg tcgccgtatt
      721 cctgcattaa cagagaccgc agcccgaccg ggagaatcct ctgcgcgagt gtgcggggat
      781 aatcaaaaac gatacacacc ggggtttacc gcgttaacgg agcgcggcgt tgtcccctca
      841 tggtcgctgg tccggtgcga tggtggaaga agccggatgt ttatcactat taattgataa
      901 cacagaaatg gattcattga ttttcagcac gtttttgtat tcgtattatt gaacatctgt
      961 ttattttact tttaacatat tgataataaa aagagctgta aatctttaga tgagtcgatt
     1021 ttgtccgagg aagttcaaat ggattttatg ctgacggttt ctggtgtggt tatcctgtcc
     1081 attgcttata ctgcagataa atatggctgc catttgttat cacgtattgg cgcttattgc
     1141 tcgttgatgc tgattttctc gtcgcttttt tttgagtaag ttatattaat tataacaaat
     1201 aattttctgt gttttttcag gctatcccgt cagacgggaa gcctgtactg ccgggggacg
     1261 aatggaaaac tgatgtgtcc ggtaactgtg tgttctgtga acaccatgtt acttaattat
     1321 gtaattcata cccgaacgct ctgttgacag ccttcttctg caggcttcaa taacccacgc
     1381 tgaaaagtta ccggaacctt tatgttcaag ggcgatattg atctgttcaa tcatgtgatt
     1441 ggggaaacgg atattgcgga ttgtggttct gcggggccgg tttttcgatg acattttctt
     1501 tcctctggtg acaagctata tggcgaggat tttacatggc tgtgcttcgt acgttaccgg
     1561 gcagaatcaa aactctgaac acccggcgga taaatgtcct gaggggggaa cagcgtcgtg
     1621 tcagtggcag tgcccgggtt tccctcaaac gtcgtatctg gcggagggat gccggacact
     1681 gttgtctctg tagacgcgtg gttgaccttt gtgacagtga actcgatcac cgcattgcac
     1741 ttcagttcgg tggtggtaat gaggagacga acctctggac cctctgtact gaatgccatc
     1801 gccagaagtc agcgagtgaa gcggcgagtg gtatgcctga tccgacgttg cctgagcttc
     1861 ctgatggcac gctcagggcc gacggaatca ctggcctgtg accagacccg ggggggatca
     1921 tccggcgaaa aaaaacgatc gccctggaca ccgcgccccc tctcacgcag agaaaaaatt
     1981 cccgtttcag ggcagttaac atgttaactg gctgcccggg catttttgcg gtttttatct
     2041 ttattattca gtttgttgtg cggaaaaaat gttaacaggc tttttcagca aatgttaacc
     2101 aggcagcagt taacatttgc ggcatgagac gccgggaaaa atgggctgaa ccatacccgg
     2161 ctgagtgcgt tctggacccg ggaggaggct gtgctgacaa cgcaaaaacg aaaatttgcg
     2221 ctggcgctca tgtccgggaa aaacaaaaca gcgtcagccc ttgccgccgg ttattcggcg
     2281 aagacagcca gggttaaagg ctcgcagctg gcaaaagatc cggaggtgct tgcgtttata
     2341 gcccgtaaac agtgcgagac ggtggaggtg gatgaggttc ctgtttatcg gcagaaaaaa
     2401 tcagagccgg aggataaacc ccgtcgccgt gaggcggctg caataccaca gccggacgaa
     2461 acaaatccgg agatgccacc gcccgtggtg atatctcctg gtattgagta tatggaggac
     2521 ggtcttcccg atccggtgaa agcgatgggg cgtcttctgg tggagaacat taataccgac
     2581 cccaggctgg cgctggatgc ggcttataag ctggcgcagt tcacgcacca caaaaaaggg
     2641 gatgccggta aaaaatcggc aaaaggtgac gcggcgaaaa aagcggctaa ccgttttgcg
     2701 gtgccaccac caccccgcct ggtggtgaat aatgataatg agggcaacgg atgatacctg
     2761 tgtggagcac ggcctgcccg gactgggcag agcgcctgaa aaaggggctg tcgattattc
     2821 cggctccgat ttatccggag caggccgcac atgccctggc gatttttaaa caactgcgga
     2881 ttgtggatgc accgggcag
//
LOCUS       DAZKIF010000278         2895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZKIF010000278 DAZKIF010000000
VERSION     DAZKIF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.280"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1890
                     /locus_tag="ACHOTW_004877"
     CDS             <1..1890
                     /locus_tag="ACHOTW_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9846799.1"
                     /translation="LAEKLTHKLKEGWQPFGSPVAITPYTLMQAIAAEGDVTTPVVVP
                     GTGDGGYPGVVTTEPDYYYVIPLAGQSNGMAYGEGLPLPQTYDRPDPRIKQLARRSTV
                     TPDGAPCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIP
                     ANAGILLVPCCRGGSAFTTGADGTYSDVTGASESSTRWGVGRPLYKDLIGRTKAALAK
                     NPKNVLLAVVWMQGEFDFDGTPANHTARFTEVVEQYRTDLADMVGQCAGGSADGVPWI
                     CGDTTYFWKQKSESTYQTVYGSYKNKTEKNIHFVPFMTDENGANVPTNKPEEDPDIPA
                     SGYYGAASRTSANWTSADRASHFSSWARRGIISDRLASAILLHAGRTAELVGGEQVVM
                     PPDEKPSPDTPSTPSTDGKSVTTLLYYRATESGGLLNPQGWGAEGGRALVVDDAGAAG
                     GKALRWTKQTGSSSWFMQHDAGNGADLLEKGGLISCRFKVDGTLTANQYALALYWPVS
                     SLPQGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNAGQNTKLGSFGAFDNEWHTLG
                     FRFAGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITSGATYPVLIESITV
                     EVNNP"
     gene            2038..2220
                     /locus_tag="ACHOTW_004878"
     CDS             2038..2220
                     /locus_tag="ACHOTW_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9846800.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            2258..2527
                     /locus_tag="ACHOTW_004879"
     CDS             2258..2527
                     /locus_tag="ACHOTW_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9846801.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            2603..2818
                     /locus_tag="ACHOTW_004880"
     CDS             2603..2818
                     /locus_tag="ACHOTW_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9846802.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2823..>2895
                     /locus_tag="ACHOTW_004881"
     CDS             2823..>2895
                     /locus_tag="ACHOTW_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFP9846803.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVA"
BASE COUNT          730 a          671 c          866 g          628 t
ORIGIN      
        1 cttgcggaaa agctgacaca caaactgaaa gagggctggc agccgtttgg tagtcctgtg
       61 gccataaccc cttataccct gatgcaggcg attgccgcgg agggggatgt gaccacgcca
      121 gtggttgtgc ccggcacggg ggatggtggc tatccgggag tggtcaccac ggagccagat
      181 tattactacg ttattccact ggccgggcag tcgaacggca tggcttacgg tgaggggctt
      241 cctctgccgc agacatatga ccgtcctgac ccgcgtataa agcaactggc tcgtcgcagc
      301 acagtgacgc cggatggcgc tccctgtaaa tataacgaca ttattccggc agaccactgt
      361 ctgcatgatg tacaggacat gagccgtctt aaccatccga aagctgacct gtcgaaaggt
      421 cagtacggaa ccgtggggca ggggctgcat attgccaaaa agctgctgcc gtttataccg
      481 gcgaatgcgg gtattcttct ggttccgtgc tgccgtggtg gttcagcttt caccaccggg
      541 gcagatggaa catacagtga cgtgaccggt gcctcagaga gttctacccg ctggggtgtg
      601 ggcaggccgc tgtataagga tctcatcggt cgtacaaaag ccgcgctggc aaagaacccg
      661 aaaaatgtgc tgcttgccgt ggtgtggatg cagggggaat ttgactttga cggaacgcca
      721 gcaaatcaca cagcccgttt tacagaagta gtggaacaat atcgtacgga ccttgcagat
      781 atggtgggac agtgcgctgg tggttctgct gacggtgttc cctggatatg tggagacaca
      841 acttatttct ggaagcagaa gagcgaatcc acttaccaga cggtgtacgg cagttacaaa
      901 aacaaaacgg aaaagaatat tcactttgtg ccgttcatga ccgatgagaa cggagcaaat
      961 gtcccgacga acaaaccgga agaagacccg gatattccgg catcaggata ttacggtgcg
     1021 gcctcccgga cgtcggcaaa ctggacgtca gcagaccgtg cgagccattt cagctcatgg
     1081 gcacgcaggg ggattatttc tgaccgtctt gcctcagcga ttcttctcca tgcaggacgg
     1141 acggctgaac tggtgggtgg ggaacaggtt gtgatgccgc cggatgagaa gccgtcaccg
     1201 gacacaccat caacaccgtc aacggacggg aaatcagtga caacgctgct ttattaccgt
     1261 gcaacagagt caggtggttt actgaatccg cagggatggg gagctgaagg agggcgtgca
     1321 ttggtagttg atgatgcagg tgctgcagga ggtaaggcgc tgaggtggac caaacagaca
     1381 ggaagttcct cgtggtttat gcagcatgat gccggtaatg gcgcagacct gctggagaag
     1441 ggcgggctta tcagttgtcg ttttaaagtt gatggcacac tgacagctaa tcagtacgca
     1501 ctggcgctgt actggccggt ttcttcactg cctcagggcg tcacactgga aggtaatgcc
     1561 ggtcataacc tgctggcgtc gttttacgta cagagcgatg ccacagacct taatgtgatg
     1621 taccacaagg gaaatgctgg tcagaacacg aagctggggt cattcggcgc atttgataac
     1681 gaatggcata cgctgggctt ccgttttgcc ggtaacaaca gtattgaggt gacgccggtc
     1741 attgatggta aggacgggac gccgttcatg ctgtcacagt caccggtcgg cacgtttacg
     1801 gcagacaaat tgcgcgtgac cgatatcact agcggtgcga catatccggt gctgattgaa
     1861 agtataacag tggaagtgaa taacccgtaa gcaggaaaaa aaggccgccg gggcagggaa
     1921 aacaaggagc cagaaccggc ggcaaatgtc gttatatcca aagcaaaaca tgcaggacac
     1981 ttttttaacc aacaggtatt aacgatgtca acaccatatc aataaccggg agggataatg
     2041 agatttgtac agcttatttt attgtatttc tgcacggtgg tgtgcacgtt atatctggta
     2101 agtggcgggt ataaggttat ccggaactat atacgcaaaa agattgatgc cgcggcggcg
     2161 gaaaaaatca gcgccagcca gtcagccgga acaaaacccg aagagcctct catttcgtag
     2221 caactttctt aacaacacct ttcaacgaga aaatcccatg tcagaaataa aatctctggt
     2281 cactgctgaa gcagtgaagg acgtcctgcg ctctgaagaa gtcagaagcg cactgaaaca
     2341 gcaactccgc cagaatcttg aggcgcgtct tgatgctgaa gtggatgcca ttctggatga
     2401 gctgctgggg ggaccggctg ctcctgagcc tgaagacggc gcgggtgaca gtgctgtttc
     2461 agatggcgtt gtgtctcagc ctgacggtag cagtgagcct cagcctggcg gcgaaatgat
     2521 gatgtaacca tacgcagggg ctgtcggtgt gagctgatgc ccctcctgtt gttgtgagct
     2581 tccggattgc gggagacggg gtatgtacca gatggaaaaa atcacaacgg gtgtgtcata
     2641 caccacgtca gcggtgggga cgggatactg gttactgcag ctgctggaca aagtctctcc
     2701 gtcccagtgg gtggcgatag gtgtgctggg gagtctgctg tttggcctgc tgacgtatct
     2761 gactaacctg tatttcaaaa tcagagagga ccgtcgtaag gcggcgcggg gagagtaaag
     2821 cgatgaagaa aaaatacgaa ctggttgtta aagggataaa taattacccg gataagatta
     2881 ctgttactgt ggcac
//
LOCUS       DAZKIF010000279         2884 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZKIF010000279 DAZKIF010000000
VERSION     DAZKIF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.281"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            284..1009
                     /locus_tag="ACHOTW_004882"
     CDS             284..1009
                     /locus_tag="ACHOTW_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9846804.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            967..1644
                     /gene="bglJ"
                     /locus_tag="ACHOTW_004883"
     CDS             967..1644
                     /gene="bglJ"
                     /locus_tag="ACHOTW_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP9846805.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1682..2470)
                     /gene="fhuF"
                     /locus_tag="ACHOTW_004884"
     CDS             complement(1682..2470)
                     /gene="fhuF"
                     /locus_tag="ACHOTW_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP9846806.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2611..2847
                     /locus_tag="ACHOTW_004885"
     CDS             2611..2847
                     /locus_tag="ACHOTW_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP9846807.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          775 a          603 c          711 g          795 t
ORIGIN      
        1 tttaattata aattaaatga atgagatttc tgtttttcat taagtatttc taacaactta
       61 atgtagatat tttaaatgtc tccaggctat ttcaccaggt tatcatttga tgctaacctt
      121 ctgacacatg cagtggagtt gttgtgcagc aggagtatgc tgatatgaaa gtgaatgcta
      181 aggataattt attcgcttaa tctattaatt tgctggaaac atttaaggaa tgaatatcct
      241 gaataaacca tcataatttt tagacagtgg atgtggagga aatatgttgc caggatgctg
      301 caaaaatgga attgttatca gtaaaatacc tgttatgcaa gcagggttaa aagaggtcat
      361 gaggactcac ttccctgaat atgaaataat atccagcgcc tctgcggagg accttacctt
      421 attacaatta cgtcgttccg gattagtcat tgctgattta gccggtgaaa gtgaagatcc
      481 acgttctgtt tgtgaacatt attattcttt aatctcacaa tatcgggaaa ttcactgggt
      541 tttcatggtg tcacgctctt ggtattccca ggctgtagaa ctgctcatgt gccctacggc
      601 gacgttattg tctgatgttg aacccattga gaatctggtc aagaccgtac gttccggcaa
      661 tacgcacgca gagcgtatca gcgccatgct gacctctccg gcaatgactg aaactcatga
      721 ttttagctat cgctccgtca ttctcactct ttcagagcgc aaggtactgc ggctattagg
      781 taaaggatgg ggcatcaacc agatagcttc attgcttaag aaaagtaata aaactatcag
      841 cgcccaaaaa aacagtgcga tgcgtcgact ggcaattcac agcaacgctg aaatgtatgc
      901 atggataaat agcgcgcagg gtgcaagaga acttaacttg ccttctgttt atggagatgc
      961 cgcagaatgg aacacagccg aattaagaag agaaatgtcg cactcataga aaaatgcgtc
     1021 atgagtagta tcggtattga gagtttattc agaaagtttg cgggcaaccc ttataagctc
     1081 catacctata ccagtcagga gtcatttcag gatgccatgt cgcggatctc gtttgcggcg
     1141 gtcatttttt ctttttctgc catgagaagt gagcgcagag agggattatc ttgcctgact
     1201 gaactggcga ttaagtttcc gcgtacccgg cgtttagtta ttgcggatga tgatattgaa
     1261 gctcggctga ttggttcatt gtcgccatca ccgctggacg gtgtattaag taaagcgtca
     1321 acgctggaga tttttcatca ggaactcttt ttgtcattaa atggtgtacg tcaggcgacc
     1381 gaccgactga acaatcagtg gtacattaac caaagccgga cgttaagccc gacggagaga
     1441 gaaatattgc gctttatgtc gcgtggctac tcaatgacac aaattgccga gcagcttaaa
     1501 cgcaatatca aaacgatccg tgcacataaa tttaatgtga tgtcgaaact gggcgtcagt
     1561 tctgacgcag ggttgttgga ggccgcagat attctgttat gtatgcggca ttgcgaaaca
     1621 agtaatgtgt tgcatcccta ttaatccgca tgatgccggg tttacttccc cggcagtgct
     1681 ttcatttcag cgtacaatcg ccacattgct gtacatccgg taagcgataa cgctggcagc
     1741 aagtgcggcg caccagcagg ccgtcgcgta gcaccacggt acgccagagt ggattatctt
     1801 caccggtagt aagcgttttc tcaaaaaaga gggcatggcg cagcgattca accgtcgcct
     1861 cgccgagcag ttgcttcatc tcagtaagat accagttgat caaataaccg gtattactcc
     1921 agataagttt gccgttgatc tctccggtcg cttctagtgc ctgcacaacc ggaaccagcg
     1981 cctggctgat taacgtttcc attcgctgct gcggcgaatg tggtgttgcg tttttatctt
     2041 cacacacatc gacccagaaa caggcgacgc gtccggtttc gtgaaactca gcatggaaat
     2101 gttccggcga cacatctaat gctttttcct gcgtcagtag cgccagcatt aatggtggca
     2161 ccatcaggcc gatataccat tgtgcccata gtgagatcag cggtttgtac tcgcggatca
     2221 tcgttggttg gttgcgatag atatgatcgg aatagaccgc cagtagagaa cttagcgcat
     2281 tcggtgatga ccattgcgcc agcgtcatgg cgttaagcgg agcgggttca tccaggcgga
     2341 taaactccag caaatgttca cgatgttcgg cgatcgtcgc acgtacggct tgtgcaagcc
     2401 tagcatcctg tggctggaga tgcgttcgcc agatgatatc ttcatagagc ggtgcggaac
     2461 gataggccat aatcgggata gtaatctaaa tgataatgat tgctaatcct agcgataggt
     2521 ttacccgata gcaagggatt tctctggctt gcaagtgata aaaattatca tatgatattg
     2581 gttatcatta tcaatgaaag agataaaacc atgttgcaac gtacgctggg cagtggctgg
     2641 ggagtgttgc tgccgggatt tctgattgca gggctgatgt atgcggattt atcgccggat
     2701 cagtggcgga ttgtcattct gatgggatta gtattgacgc cggtgatgct gtatcacaaa
     2761 cagttgcggc attacatttt gctaccatcg tgcctggcgc ttattgctgg catcatgctg
     2821 atgataatga atttgaatca gggatgaaaa aacagggaag aaacaagaaa ggaagtaaag
     2881 ataa
//
LOCUS       DAZKIF010000280         2882 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZKIF010000280 DAZKIF010000000
VERSION     DAZKIF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.282"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            710..901
                     /locus_tag="ACHOTW_004886"
     CDS             710..901
                     /locus_tag="ACHOTW_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9846808.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(954..1187)
                     /locus_tag="ACHOTW_004887"
     CDS             complement(954..1187)
                     /locus_tag="ACHOTW_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846809.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(1283..1906)
                     /locus_tag="ACHOTW_004888"
     CDS             complement(1283..1906)
                     /locus_tag="ACHOTW_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9846810.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFRFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1995..2504)
                     /locus_tag="ACHOTW_004889"
     CDS             complement(1995..2504)
                     /locus_tag="ACHOTW_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846811.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          864 a          718 c          596 g          704 t
ORIGIN      
        1 cgtgggtgga ctggtataac aatcgacgat tgctgggaag gctgggtcat atccctccgg
       61 cagaagcaga aaaagcttat tatgcttcca tcagaaacga tgatctggca gcctgagttc
      121 acagataaaa cactctccag gaaaaccggg gcggttcacc agcctcctga cgatacctcc
      181 caccatgttt tgcgatggca cgctaccgcc cttccggtat ttacccgcat caaccggtac
      241 cagaatgctg agtaacaaac cagctagaat tgacttaatt ttgatcatta tagaataatg
      301 atctgaacta cagcaacatg acagatttat cgatcgatat aacacaaatg aacagttctt
      361 tattctgaca aatatcgcaa aaacagcaca ttacaaaatt tataaccata attactggaa
      421 aagtgcagaa aattattcca ctgaatgctt ttaatacagt tggttagctt aaaaaatagc
      481 aatttttgat ttcagtcatt gaaagttgca atgtttgacg attcaacagc atttaagtga
      541 gtttcaggat atgggaagga catgaaagga ggagcgcaat aaaatacaca ggaagaaggg
      601 gaaaatgttt cgtaaaaaca gaaaattaac agcgcacatc aggaaacgca gtctcctgat
      661 tcagtgctac cacatgaaaa tttagttaac tgttaccgta ctaaaattta tgtcagaaga
      721 tgttccatta ccgaaagtca atcagcgata caaagacgat catggtgcgc ttgtcaccct
      781 gaccagtgtt gaggaaaccc gggtggtgtt tatgcaggac ggctatccgc atccctgcat
      841 gcgaccgatg tacaactttc tgggaaaatt caaacccgaa ccccgggaag aaacagaata
      901 aaacgaaatc cctgccggca atcacactac ctgatagcag cataatccac ttattaattc
      961 tcatccccgg agacaagccc agaaaaccac tccgggtgtt cactgtatgc cgtgttcagc
     1021 gcatccctgg aaacgtttat ataaaagctg tcctgcaatt tgcgggtaac aacttctgcc
     1081 aggcgattcc ggttatccac cagtccggca taacctctct ctgccagcca ctcattcagt
     1141 tcatgttcca gagacagatt gataatatca taatcggacc ttgccattcc cgtttcctta
     1201 gcacatgttt tcaggcacgg ggataaagta taaagaatgt catactgctg ctgccagagc
     1261 aatattcccc ctgatggttg tatcagagat ggtatgccgg tactttgcgg gttgttcccg
     1321 tcaggttatc gtgcacctcc acggtcgtgg tcaccaccgg cattccggcc tttctcagcc
     1381 tcagaacatc agccgcaata cgctgactgc caaacctgaa caggccgtcc agtgcagtca
     1441 ccagtaaacc cgcctccagt gcatacttca gccgttcacg gggcgctttc acttcccggg
     1501 caacctgctg atatggcaat gatgtgtttt cattcccaac cacgcgacga atacggtgag
     1561 acagatgata ccggtatgta tccggcacac cagaaaggct ggccttcagg ctgtacacgc
     1621 agccaaaccg tttatcgtta aacaccacat ttttctggct gatgccccat tcttcacgca
     1681 gcgcggcaat caattgcggt gtacgggtaa gcagcaagcg aaaaggcagt tcaaaactgg
     1741 tgacgtaatc cacattcagc agtgcaatac gcaaccgttc ttctggcccg gcctctgtct
     1801 gccggcactc ctccagaaca tcctgccact gccggcgaag acggggggat tcgttccgtt
     1861 ctgtaaaaca gtatttatcc gccagatagt caattcgtgt acgcatactg aaaggaattc
     1921 cgtataaaga ttcagcagac aaaactttat cagccagtaa aaattaacgg aagcattgat
     1981 attcctcccg acaatcaccg gatgattgtt gcaataacct gcgacataaa ggactgaaca
     2041 gccgttttta acgccacgta ttgccctgat gaaccaggcc gggccacccg aaaacatctt
     2101 tccacccggc attgtccgcc agcacttatc accgaccagg tctgttgcag taaatccacc
     2161 agttgcgcac attcttcatc agccatgacg ccgactcgcg gatggcaaat actcgtctgg
     2221 ctgagcagca acagacatcg tatctgtgct ccttcatgtt gttcccgcaa ccagcgtaat
     2281 accaccggcg ctgatgggct gaccgtgtga ttatgctccg taatacgttc gtgtacccgt
     2341 cggtgaaagg aactacaaaa tgtctgaatc tgttgaccat agattccttc tgccgaatag
     2401 agcgtgaagt gaaatgccaa cagatttggc cagtggtcca ccgcctgctg aacaaaacgc
     2461 cggatttccc ccggctctga aattaaggct tcggttattt gcacgaaatg ttccttttca
     2521 acccctgttg aattttgtca agccggtgca gacacagtaa agcgcgcatc aacacctgaa
     2581 aggtacgata caaacacaag ttacaaacaa taagaacgga aggacaatta atcagtgagg
     2641 aataataact aaattaacca aaagaatgtt actgacaata ctaccaatac ttaaatagca
     2701 atatattaat taatgtaaga acagatgccc ggtaggacat atacacaata catccacaaa
     2761 cacactcttt tcattaaatc aatcacctga ttaaaccatg aaagtgtgat gtaagtcaaa
     2821 attactgata ctatcaattg ataatgatta tctctatcat tatcgtgaaa ttcgagaatg
     2881 aa
//
LOCUS       DAZKIF010000281         2818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZKIF010000281 DAZKIF010000000
VERSION     DAZKIF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.283"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHOTW_004890"
     CDS             complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHOTW_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP9846812.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
     gene            complement(399..1022)
                     /locus_tag="ACHOTW_004891"
     CDS             complement(399..1022)
                     /locus_tag="ACHOTW_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9846813.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(1025..1306)
                     /locus_tag="ACHOTW_004892"
     CDS             complement(1025..1306)
                     /locus_tag="ACHOTW_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP9846814.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1299..1625)
                     /locus_tag="ACHOTW_004893"
     CDS             complement(1299..1625)
                     /locus_tag="ACHOTW_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFP9846815.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(1713..>2818)
                     /locus_tag="ACHOTW_004894"
     CDS             complement(1713..>2818)
                     /locus_tag="ACHOTW_004894"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP9846816.1"
                     /translation="KESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKDNA
                     YNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQGWE
                     ESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALATY
                     GEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFHAD
                     HKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVPVALETVANQTIKSASVK
                     GADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYIDQ
                     QEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
BASE COUNT          593 a          895 c          684 g          646 t
ORIGIN      
        1 gtaattttcc acaggagatc tgccgatccc cacatcataa actcatcatc ccggcggtag
       61 tcatacccct gaagattcat cttcagcagt aatgcactga gcccgggaac tgcctccagc
      121 gcaggaagaa ttttttcttc catccacata tccagtgccg agtccggttc ttttgccctg
      181 agaaaaacct caatatgcag tgtcgcctcc caggttccct catcaacgaa ctcgtcagca
      241 gcagacgcat ctgtcaggta aacagcaaca gcaggcagtt cctgttcatc aataaaaacc
      301 gggcgaccgt caaaccagct caccctctca gaaatatttt ctttcagggc agacagaact
      361 gccgcccgta tttcacggtg tttcatacac cctcccagtc attttctttt cagcaccagg
      421 cgtaactgat gcgtcatggc tttcatcatc tgcgccggta atttttcccg gtacatccgg
      481 tcccgttcac gttcaaaggt ttctgccagc ggtccggcag tcgtaatctt caccacttcg
      541 atcggcagac ggtggcgttt cggcctccct ttgctgtccg cgccggtgga tgatgatgcc
      601 cacggcatac gctgcatcac atgccagcgt ccgttagcca gccgggtgat aaaggcgtcc
      661 gggatccgtc ttttccccac aatcagcaca ctgccgcccc ctttcagggc cgcacgctgt
      721 cctttctttc tccgttttct gcgggaaagt cgaacgcggg cctcccccag tttgatggcg
      781 ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct tatccggtcg tgccggactt
      841 aaccggatgc gggcacggat aagacgacgg ggaacggcca gctccctggc aactgaagag
      901 gccgttttcg caataatggc ccccgccact cggttcagtg tcgtggcaga ggcccgggga
      961 acggcacggc gatcaattgc atccagattt ttcatggcct gcgccagacc ttttattgcc
     1021 atactcattc ctgttcgaca aaaatccggg gtttaccgtt gtacgtgtca taacgggtca
     1081 ccgtcagtgt gcgccccgca aacacaacaa catcatgacg ggccggacgg taccgggctg
     1141 aaaacaccac cagtgacaac tggctgcccg aaagcgcccc catctccgcg gactcttcct
     1201 ccggcatcac gtcgtacacg acgccgttaa tctccgcctg tttgcccatc acccgaacgg
     1261 tcgccacgtc catccggcaa cacattcgcg taaacagatc agacattgat ttttaccgcc
     1321 acagtggcgc tgtttgcagg ggcattttcc caggctaccc ccgctgccac cgcaccgtct
     1381 gcagccagct gcacaacccc gtccttcaga taaaccaccg cgccggactg aatgtcgtca
     1441 gcagactgtt tgggcagcag gaacacgcct tcggcaaaac cgtcaccggc ctcaccggca
     1501 ggaatatcgg taatggccac ggccaccata ctgccgacca ccaccgcagc accgctcagg
     1561 atggtctgat ctccggcatt caccagttca atggtggtac cgtcctgtac aaaatttttc
     1621 gccataatgc tgtttctccg gacagcccct gtggggctgt ttttcaggca taaaaaaagc
     1681 cctttcgggc agtgattgtg ataacgcggt tatcaggcca ccgacgaacg caccagcccg
     1741 cgccagtcaa gtggtgccac tccggcatca atacggattt ttgtggcaat gccgtcagtg
     1801 gtgaaacctt cctgctgatc aatgtatggc gtgtccacac catccagcca ggccacttca
     1861 atggtgtcag tgccctgtgc cgccgccaga taccaggttt tcgggtctgc cgcatcaaga
     1921 cgcgcttctg caatcacctc agcaaagttc tggatagggt taatgacacc ggcgtttgca
     1981 tccgcccctt tcacactggc cgatttgatg gtctggttcg ccaccgtctc cagtgccacc
     2041 ggtaccagca taaaggccgg acggatattc agggcacgat cgccttcttt ctgcaggcgc
     2101 atcatctgac gggccgcatc cagtccggaa acggagatcc ccccggtggc aatatttttg
     2161 tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg ggttatccgt cagcaccttg
     2221 tagaccaggt caccaatcgt tgccttcgcc gcacgcccca tcttcatcgg cacgtccacc
     2281 agcatattca gatcatcatt gataatggcc tggcgggtga tggagaaaat ctccccgtaa
     2341 gtggccagtg caatggtctc cttgcgatct gaggtggtga tgtatttata ctccgccccc
     2401 tcacgaacct ggcgcagaga accaaaaccg cccatcccca cgcgatacgc tgttttgaag
     2461 tctgacaggc gtcccttacg ggtccacttc tggaaggttt cttctgattc ctcccagccc
     2521 tggatcagcc ccttgttcga cacatccagc agaatattgc caaaatcaga ggtgctgtgc
     2581 gtcagcgcca gcccgaccat ctgcatgggg ttataactgg ccaccccaat accgcgctcc
     2641 gtcagtgaca tgcgagccca ttcacgcagg gtcatcccgt tataggcgtt atccttctcg
     2701 acattttcaa atccggcacg ggccagcatc gcctggcgga tcccgtcccc cacaaaattg
     2761 ccgtttccgg cataaatatg ggccggtgta tttttgttgg tcggcgagga ctccttgc
//
LOCUS       DAZKIF010000282         2805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZKIF010000282 DAZKIF010000000
VERSION     DAZKIF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.284"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            37..795
                     /gene="paa"
                     /locus_tag="ACHOTW_004895"
     CDS             37..795
                     /gene="paa"
                     /locus_tag="ACHOTW_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFP9846817.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1131..1286
                     /gene="hokD"
                     /locus_tag="ACHOTW_004896"
     CDS             1131..1286
                     /gene="hokD"
                     /locus_tag="ACHOTW_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9846818.1"
                     /translation="MKQQKAMLIALIVICLIVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1507..1764
                     /locus_tag="ACHOTW_004897"
     CDS             1507..1764
                     /locus_tag="ACHOTW_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846819.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1834..2112
                     /locus_tag="ACHOTW_004898"
     CDS             1834..2112
                     /locus_tag="ACHOTW_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846820.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2114..>2805
                     /locus_tag="ACHOTW_004899"
     CDS             2114..>2805
                     /locus_tag="ACHOTW_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9846821.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQ"
BASE COUNT          737 a          655 c          738 g          675 t
ORIGIN      
        1 actttatttc atgataacca ataaaaggag ttttttatga ggaacataat ggcaggtttt
       61 ttaatattcc tgtcttctgc tgcttatgct gatatcaatc tgtatggtcc tggtggcccg
      121 catacagcct tgcttgatgc agccaaactt tatgccgaaa aaacaggtat tatagtgaac
      181 gttcattacg gcccacagaa caaatggaat gaagatgcca aaaaaaatgc agatatcttg
      241 tttggcgcat cagaacaatc tgctctggct atcattcggg accataaaga cagcttcagt
      301 gaaaaagata ttcagcctct ttatctgcga aaaagtattt tactggtaaa gaaaggtaat
      361 cctaaaaata tccggagtat tgacgacctg accagacctg ggattggcgt aattgttaat
      421 gatggtggtg gtaccagtaa tacatcaggc actggcgtct gggaagatat tgccggacgt
      481 aaagggaata tagaaactgt cgccgcaatc cgaaaaaata ttattttata tgcgcccaat
      541 agcggaactg cacgtaaggc tcttgagaat cagcctggag cagatgtctg gataacctgg
      601 gctgactggg cagccagtaa tccagaaatt ggtgatgtcg tggaaatagc gccagactac
      661 gtgatatggc gtgatatgaa cattacagta cgtcaggatg caaatgatga aacccgtcga
      721 tttgcagaat ggctacaaac cgatgaagcg gcgcctgcat tcaaaaaata tggctggacc
      781 aggaaaggca cttgacatcc tcgtccttca ggacgtggat tctttttccg gatgccgcgc
      841 cagcggcatg taggggcagc tcacaaaacg gaaaaaattg tacgctaagc ctcgccaggt
      901 gaactgaatt cattccgata tgggaattcc catatcgggc gaaaacggtt tgctgtaacg
      961 gcagagttaa gtaggattgc tgcgggtgct tgaggctatc tgcctcgggc atgaacacca
     1021 acggcagata gataaaagcc ccacccgact ataaatcgaa gtgaggcccc tatatgctcg
     1081 tcacatatag attgcctctt acggaccgaa aggtcaagga gaagcaggct atgaagcagc
     1141 aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaat cgtcatagtg acggcactgg
     1201 taacgaggaa agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct
     1261 tcacagctta cgaacctgag gagtaagaga ccaggcgggg gagaaatccc tcgccacctc
     1321 tgatgtgtca ggcatcctca acgcacccgc acttaacccg cttcggcggg tttttgtttt
     1381 tattttcaac gcgtttgaag ttccggacgg cgccggaata gaatcaaaaa tacttaagta
     1441 gcgcgcaggg agaagaggga tggaccccga acaggggagt gctatttatc tggaaggatt
     1501 ctgttgatga gaatcgaaga attacgtgaa atttttagtg aagatggcct ctatactgtg
     1561 cgcgttgaga agggcgctat tgtcagccac tgccgtatta aatgtttaca gtctcaacaa
     1621 aggaagagtg gagctgcgtt aattcatttt gtggatgggc ttgtgacgga tggttttatt
     1681 ttgcgtgcaa atgaatttgt cacatcgttg ccgtctctga aagaagctgg gattaaggct
     1741 ggtttttctg cttttgaaga ttagtgaatt catctacaat tcagcgcagg gctgaacccc
     1801 tgttgagtaa cactgtgcca ccggagaaag ccgatggcgc aaaattccag actacacaat
     1861 tctgataatt cagccgtctt tgccagcagg cacgggcggc gttctcatgc attcaaatct
     1921 gactggttcc ggcacgcccc atgcactgaa gaacaggccg aatggctgat tcagaactac
     1981 cgcagacgtg ggtatgagtt taggaaagcc ctcagcctcg attatcgtca ctggataatc
     2041 tacgtcaggc tgccgtactc cgagcgccca ccgcgtccgt cccgcacatt ccagcaacgc
     2101 atctggaggt aacgtgcggg tattacttcg acctgttccg gtaccggaac ttggggtggt
     2161 ggtccttaaa ccaggccgtg aatccatgca ggtattccat aaccctcgag tgcttgtgga
     2221 gccggagccg aaaagcatgc gcggtctgcc gtccggcatc gtccctgccg ttcgccagcc
     2281 gctggcggag gataaatcat tactgccgtt tttcagcaat gagcgtgtga ttcgtgctgt
     2341 tggtggcgct ggtgcactgt ctgactggct gttgcgtcat gtcaaatcct gccagtggcc
     2401 tcatggtgac tatcatcaca gtgaaaccgt tattcaccgt tatggtaccg gcgcaatggt
     2461 gttgtgctgg cactgcgaca accagctgcg tgaccagaca tccgaatcac tcgagcaact
     2521 tgctcatcaa aacctgtcag catggatgat tgacgtcatc ggtcacgcaa taagcggtac
     2581 gcaggagcgt gaattatctc tggctgaatt atcctggtgg gcggtccgca atcaggtggc
     2641 ggacgcgcta ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg aaaaaatccg
     2701 ctcaatgtac cgtgaaagcg acatcgtacc gggagagcag accgccacca tcatactgaa
     2761 gcagcgcaca aaaaatcttg cgccgctgcc tcacgcccac cagca
//
LOCUS       DAZKIF010000283         2804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZKIF010000283 DAZKIF010000000
VERSION     DAZKIF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.285"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1229)
                     /locus_tag="ACHOTW_004900"
     CDS             complement(<1..1229)
                     /locus_tag="ACHOTW_004900"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9846822.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTAL"
     gene            complement(1210..>2804)
                     /locus_tag="ACHOTW_004901"
     CDS             complement(1210..>2804)
                     /locus_tag="ACHOTW_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9846823.1"
                     /translation="STIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGESVDA
                     ALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEEEAR
                     AFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSR
                     LFRTQFRMVSPKRISNPNNTSDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTW
                     IPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYA
                     ATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGD
                     SLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAY
                     FMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRM
                     AIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWA
                     AAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          536 a          901 c          775 g          592 t
ORIGIN      
        1 agcgcagtat cactgcgcgc ctgtgcactc tgtggtgctg cggccagaat gcggcgggcc
       61 gtttccacgg tcataccggg ggtttctgcc agcacgcggg cctgttcttc gcgtccgtga
      121 gcctcctcac agttgaggat ccccataatg cggctgtttt ctgccgcaac cgcagcggtg
      181 atctgcgcgt tcacgtccgg ctgcgccgcg ctggcgtttt cgccctccgt cgctggcacc
      241 acgccagtaa cgtcagcctg cgaagcagtg gctgaaacag ttgttgattg agtctctttg
      301 gtcattcgcc ctcctgagag acgggattta cgtgcatcca gtgcatcacg cataacggtg
      361 atcgcatcgg tgctgttgac aagttcatca gccagtccgg catcaatggc ctcctgaccg
      421 ctgtacactg cagcctcggt atccagcaca gcctgcacgg acaggccggt atatgccgac
      481 accttctgcg caaacatccg gcgggttgca tccatccggg actgcagtgt ttcccggaca
      541 tcacccggta gatggctgta ggggttgcca tccaccttat ggctgccgct gtaaatcagc
      601 gtgatttcca cgccctgttt ctccagggca gcaccgtaat tactgtgagc catcatgacg
      661 ccgatggagc ctgtccgggc ggtctgcgtg accagacgcc gggaggcggc gctggcaagc
      721 agctgacctg cactgcagtt catgtcgttg gccagcgccc ataccggttt tatgtctcgc
      781 acacgggcga tgatgtcagc acagtcaaat gctcccgcca ccatcccgcc cggtgtgtcc
      841 atatcgagca gaatgccgtc caccatcgga tcgctggcag cctgttgcag acgggcgata
      901 atgccgttgt aaccggtcat tcccgaatac ggctgcagcg cccgcgtccg gctgaccagc
      961 gtaccggaca ccggcagcac ggcgatgccg ttcatgacct gataactccg ggcctgtcgt
     1021 ggtccgtcat catcaccgga taacgccagc gccgcgggtg cctctccggc agtcaggctg
     1081 tcgccggata ctgcatccgt caggcggctg atcccaagct ggcctgcaag cgcacaaaag
     1141 aaaacccgcg cataggcggg ttcaagcatc agcggctcat taaaagccat gctggcaata
     1201 tgcgggagat tacgcagctc tgctgtcact cttctcctcc tctgttgatt gtcgcagccc
     1261 ggattcaaat gccgcagccg cccaggcggg cggtttaaga ccagctgcgc ggcgctccat
     1321 cgtttcacgg acctgctggg caaaaatttc ctgatagtcg tcaccgcgtt tcgcgcactc
     1381 tttctcgtag gtgctcagtc cggcttctat cagcatcacc gcttcctgaa cttctttcag
     1441 accatcgatg gccatacgac cggagcctat ccagtcgcag ttcccccagg cactgcgggc
     1501 ttcctgaaag ctgaagcgcg cttttgaagg taacgtcacc acgcggcgaa cgatggcctc
     1561 ttccagccag cacagaaaca tctggctcgc ctgacgggat gcgacgaatt ttcgccgccc
     1621 cataaagtac gcccacgact cgttcgcact ggcccgtgcc gtggagtagc tcatctgggc
     1681 gtaattccgg gaaagctgct catacgagac acccagcccg gcagcgatat accgcagcag
     1741 tgactgctca aacacggagt agccgttatc cgtgtcctga gccgtctgca ggttcagtga
     1801 gtcccccggc atcaggtgcg gcacttttgc gcctcccaga cggaccggtg ctgcggcgta
     1861 atacgcggca atttcaccaa tccagccggt cagcctttcc cgctgctcct gactgttcgc
     1921 gcccagaata aaatccatcg ctgactgcgt atccagctca ctctcaatgg tggcggcata
     1981 catcgccttc acaatggcgc tctgcagctg cgtgttctgc agcgtgtcga gcatcttcat
     2041 ctgctccatc acgctgtaaa acacatttgc accgcgggtc tgcccgtcct ccacgggttc
     2101 aaagacgtga atgaacgaag cgcgcccgcc gggtaactcg cggggtatcc atgtccattt
     2161 ctgcggcatc cagccaggat acccgtcctc gctgacgtaa tatcccagcg ccgcaccgct
     2221 gtcattaatc tgcacaccgg cacggcagtt ccggctgtcg ctggtattgt tcgggttgct
     2281 gatgcgcttc gggctgacca tccggaactg tgtccggaac agtcgcgagg gacgggtatc
     2341 ccaggtggcc tgaacgaaca gttcaccgtt aaaggcgtgc atggccacac cttcccgaat
     2401 catcatggta aacgtgcgtt ttcgctcaac gtcaatgcag caacagtcat cttcggcaaa
     2461 ctctttccat gccgcttcaa cctcgcggga aaaggcacgg gcttcttcct ccccgatgcc
     2521 cagatagcgc cagcttgggc gatgactgag ccggaaaaaa gacccgacga tatgatcctg
     2581 atgcagctgg atggcgttgg cggcatagcc gttattgcgt accagatcgt ctgcgcgggc
     2641 attgccacgg gtaaagttgg gcagcagggc tgcatccaca ctttcacccg gtgggttcca
     2701 cgcccgcaac tgcccaccaa atccgctgcc accgccgtga taaccggcat attcacgcag
     2761 cgatgtcatg ccgtccggcc ccagaagggt gggaatggtg gaca
//
LOCUS       DAZKIF010000284         2801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZKIF010000284 DAZKIF010000000
VERSION     DAZKIF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.286"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..570
                     /locus_tag="ACHOTW_004902"
     CDS             <1..570
                     /locus_tag="ACHOTW_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9846824.1"
                     /translation="KTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQL
                     HETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMT
                     GILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYG
                     EMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            631..>2801
                     /locus_tag="ACHOTW_004903"
     CDS             631..>2801
                     /locus_tag="ACHOTW_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9846825.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTP"
BASE COUNT          604 a          738 c          921 g          538 t
ORIGIN      
        1 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggtgtt tcgtcagaag
       61 ctgagcgacg gctggtatca ggtacggatt accgggcggg acgtcagcac gtccgggtta
      121 acggcgcagt tacatgagac tctgcctgat ggcgctgtga ttcatattgt tcccagagtc
      181 gccggggcca agtcaggtgg cgtattccag attgtcctgg gggctgccgc cattgccgga
      241 tcattcttta ccgccggagc cacccttgca gcatgggggg cagccattgg ggccggtggt
      301 atgaccggca tcctgttttc tctcggtgcc agtatggtgc tcggtggtgt ggcgcagatg
      361 ctggcaccga aagccagaac tccccgtaca cagacaacgg ataacggcaa acagaacacc
      421 tatttctcct cactggataa catggttgcc cagggcaatg ttctgccggt tctgtacggt
      481 gaaatgcgcg tggggtcacg cgtggtttct caagagatca gcacggcaga cgaaggggac
      541 ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa accgcctccg
      601 ggcggtttta tcgtttatgg agcatgacga atgggtaaag gcagcagtaa ggggcatacc
      661 ccgcgcgaag cgaaggacaa cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc
      721 agcgaagggc cggttgaagg tccggtggat ggattaaaaa gcgtgctgct gaacagtacg
      781 ccggtgctgg acagtgaggg gaataccaat atatccggcg tcacggtggt gttccgggcc
      841 ggtgagcagg agcagacacc gccggaggga tttgaatcct ccggctccga gacggtgctc
      901 ggtacagaag tgaaatatga cacgccgatc acccggacca tcacgtcggc aaacattgac
      961 cgtctgcgtt ttactttcgg cgtgcaggca ctggtggaaa ccacctcaaa gggggacagg
     1021 aatccatcgg aagtccgcct gctggttcag atacaacgta acggtggctg ggtgacggaa
     1081 aaagacatca ccattaaggg taaaaccact tcacagtatc tggcctcggt ggtggtggat
     1141 aacctgccgc cgcgcccgtt taatatccgg atgcgcagga tgacgccgga cagcaccaca
     1201 gaccagctgc agaacaaaac gctctggtcg tcatacaccg aaattatcga tgtgaaacag
     1261 tgctacccga acacggcact ggtcggcgtg caggtggact cggagcagtt cggcagccag
     1321 aaggtgagcc gtaattatca tctgcgcggg cgtattctgc aggtgccgtc gaattataac
     1381 ccgcagacgc ggcaatacag cggtatctgg gacggaacgt ttaaaccggc atacagcaac
     1441 aacatggcct ggtgtctgtg ggatatgctg acccatccgc gctacggcat ggggaaacgt
     1501 cttggtgcgg cggatgtgga taaatgggcg ctgtatgtca tcggccagta ctgcgaccag
     1561 tcagtgccgg acggctttgg cggcacggag ccgcgcatca cctgtaatgc ctacctgacc
     1621 acacagcgta aggcgtggga tgttctcagc gatttctgct cggcgatgcg ctgtatgccg
     1681 gtatggaacg ggcagacgct gacgttcgtg caggaccgac cgtcggataa ggtgtggacc
     1741 tataaccgca gtaatgtggt gatgccggat gatggcgcgt cgttccgcta cagcttcagc
     1801 gccctcaagg accgccataa tgccgttgag gtgaactgga ttgacccgga taacggctgg
     1861 gagacggcga cagagcttgt ggaggacacg caggccattc tccgttacgg tcgtaacgtc
     1921 acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg
     1981 ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtggg cgcagaaggg
     2041 cttcgccatg taccgggcga tgtcattgaa atctgtgatg atgactatgc ggggatcagc
     2101 atcggtgggc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa
     2161 atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg
     2221 gtcagcgtgg aggtccagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct
     2281 gacggcgttg ccggatacag cgtatggggg ctgaagttgc cgacgttgcg ccagcgcctg
     2341 ttccgctgcg tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag
     2401 catgtacccg aaaaagaagc catcgtggat aacggggcgc actttgacgg cgacctgagc
     2461 ggcacggtga atggcgtcac gccgcccgcg gtgcagcacc tgactgccga agtcaccgca
     2521 gacagcgggg aatatcaggt gctggcgcgc tgggacacgc cgaaggtggt gaagggggtg
     2581 agcttcctgc ttcgcctgac cgtggcagcg gacgatggca gtgagcggct ggtcagtacg
     2641 gccaggacga cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg
     2701 actgtccggg cggtaaatgc gtggggacag cagggcgatc cggcatcggt atcgttccgg
     2761 attgccgcac cggcagcgcc gtctcggatt gagctgacac c
//
LOCUS       DAZKIF010000285         2793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZKIF010000285 DAZKIF010000000
VERSION     DAZKIF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.287"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..259
                     /locus_tag="ACHOTW_004904"
     CDS             <1..259
                     /locus_tag="ACHOTW_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9846826.1"
                     /translation="SLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGFVSSKKIDLNC
                     LSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
     gene            complement(352..711)
                     /locus_tag="ACHOTW_004905"
     CDS             complement(352..711)
                     /locus_tag="ACHOTW_004905"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9846827.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            1397..1687
                     /locus_tag="ACHOTW_004906"
     CDS             1397..1687
                     /locus_tag="ACHOTW_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9846828.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            1711..1962
                     /locus_tag="ACHOTW_004907"
     CDS             1711..1962
                     /locus_tag="ACHOTW_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9846829.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(2010..2711)
                     /gene="traT"
                     /locus_tag="ACHOTW_004908"
     CDS             complement(2010..2711)
                     /gene="traT"
                     /locus_tag="ACHOTW_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFP9846830.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFEEAK
                     PHLEDQLAKSIANIL"
     gene            complement(2662..>2793)
                     /locus_tag="ACHOTW_004909"
     CDS             complement(2662..>2793)
                     /locus_tag="ACHOTW_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9846831.1"
                     /translation="SRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          771 a          571 c          600 g          851 t
ORIGIN      
        1 atctctggga gaaaatggat tatacttaca tggaacacca tataatgttg ctccagctga
       61 gcaaacgcat gtgcctctta ttacctggat gtctccgggg ttcgtttcct ctaaaaaaat
      121 tgatttgaat tgtttgtcag aacatgctct aaacagaaca gtaagtcatg ataatatatt
      181 ttcatcgtta ctggggttat ggaatgtaaa tacttcagtt tataataaag aagatgatat
      241 tatatcctcc tgcagataga tactgacatt tttctgagga taagaggtga atacctctta
      301 tccttaagta aatattaacc aggtaactgt gaataattta gatgttgggc gttatgaaag
      361 taataaaact ccccatataa tgatagcaac taaaatcgta atcaacactg cggctgaacc
      421 catatctttt gctctgcctg ataattcgtg atgttctgaa ccaattctgt caacaactgc
      481 ttcaattgca ctgttaatta tttcaacaat aattaccatc attgccgaac caatgagtaa
      541 tactcgagta acagagtcaa catccagcca aaaggatatt atcagtgctg acagaaccag
      601 aatgcactcc tgacgaaagg ctgcttcata gcgccatgcc gcacataagc ctttccatga
      661 ataacccgct gctttaataa ttctggtaac accagtggtc gtattagcca taaatacctc
      721 ttatttaatt atatgtctga atatgcatta taaaatatca gttaacatta acaggatttt
      781 gtaataagcc acatttattc tttcgggaat taaaaaacat aacgaaattt tgcctcattc
      841 tgcatttcat tgaaatagta tgtttctcat cgaaagcaag tttgtcaata taaatttgta
      901 acagaaatga ctttccgggg ataaacaaaa ctgttcatgg caatggcttt ctctgtcgcc
      961 aacatggata cattgttccg gataaagcac tgatgtcatt gactgacgca gactatcagg
     1021 ccatctgatg ctttcttttc cttcaccggt attgatgata tgccttatga agggggactt
     1081 attcctgtaa cccaatcaca ggcactgtga ttactggttt atgattgatg ctgatttgag
     1141 cagatatgca cagcagttgt ggattcaggg gatattttct gccagatatt cataccagat
     1201 tgatgacctc tttgctcaga tgctcttcgt tctgagccag ccaggatttt gtggccacat
     1261 acattgttcg ccgatgggga atcgagacag gcgattggct gtgatgcgat tttgccgcta
     1321 actgaggatg agtccgcccc tcccggggaa ttcctgtaag ttaagccgta cctgttgatt
     1381 agcactaaac tgggtaatga atttatctgc cagtatgaat gatgccgggg acggttttga
     1441 actcctgatg tcgggtaata tgcaggattt taatatttgt tgtcgtcggg ataatcatta
     1501 tcctgaacat aaactgatac ctttatcaga gttatttgac gttaattatg gactgaatct
     1561 tgagctgaat aaactggaga aagattcttc aggaattaat tttgtttcaa gaacgtccaa
     1621 aaataatggc gtctccgcca gggttaaatt aaaagatggt attagtccat gccagcagct
     1681 gtgttaactg tagcagcagg aggttctgtt atggaatcgt ttgtacagga ttcaccattt
     1741 tatagtggaa gagatcttta ttggttacgt cctaaagttg aactgactct ggaagagaaa
     1801 ttgtactact gctcttgtat aagaagaaac agacacaaat acagctacgg gcgtcaggca
     1861 aaccgaacgc tgaagaattt actggtaccg tcattagatt ctgttccggc atgggtatat
     1921 ggtgtgacag ggaaaataat atctgaattg tctgaacgtt gagactaaga gggccggagg
     1981 ccaggtgaat ggccaccggg atatttatat cagagtatat tggcgattga ttttgccagc
     2041 tggtcttcca gatgaggttt agcttcttca aacttcagat taacctggtt ggcactggat
     2101 acaacccggg tctggtattt atgctggttg ccggtttctg tgcttgtctg aattttggag
     2161 cctgatgttc cctgacgcaa agctgcaaca ttatccgttc tgacgctggt tctggttcgc
     2221 tcggcaatct gaacatctgt aatcattgtg taatttatat cttccaccat tgcatccgcg
     2281 gccatgccaa tcagaccggt agccagtcca acaccgagag tggtacctgc cgacccggta
     2341 ttgtacgctg tgattccacc tccaagagca gctccgactg ctgcaccttc ataaccgcga
     2401 ctcagccatc cctgagattc acgcagatcc attttgtctg ctttgagaac gttagcctgg
     2461 atccagtagt aagctttatc cggggaagtc atgatctgat aacctctggc tttgactgca
     2521 tcggcaattt ttccctgtag tccactcatg tctttatccg acgtgttttt gatctgcagg
     2581 aaaacggtgc gttcgctcgc aggttcaagc cagatggttg aggtgtactg acaatagcgg
     2641 acactaccat ttgttctttt tttaagcagc catctgatga tatttttccc tgaaggctgc
     2701 cggggagata ttccccagac gagagtgacg acgctgacga ttgtagaaaa tctcaatgta
     2761 ttcccgtatt actgagatgg cctcatcccg gct
//
LOCUS       DAZKIF010000286         2777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZKIF010000286 DAZKIF010000000
VERSION     DAZKIF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.288"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..546
                     /locus_tag="ACHOTW_004910"
     CDS             <1..546
                     /locus_tag="ACHOTW_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9846832.1"
                     /translation="KTGAEAIRALAMQIPAFRQKLSDGWYLVRIAGRDTGENELSARL
                     NEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGA
                     SMILGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            607..>2777
                     /locus_tag="ACHOTW_004911"
     CDS             607..>2777
                     /locus_tag="ACHOTW_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9846833.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALRETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYDPEKRTYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEGIVDNGAHFDGDQSSTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTP"
BASE COUNT          603 a          714 c          933 g          527 t
ORIGIN      
        1 aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggcgtt tcgtcagaag
       61 ctgagcgacg gctggtatct ggtacggatt gccgggcgtg atacaggtga aaatgaatta
      121 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt gccgcgcctg
      181 gcgggtgcta aaagtggcgg tgtgtttcag gcagtgctgg gtgcggcgct gattgcggtg
      241 gcatggtgga accctgtggg ctggctgggg gccgcggctg tatcgggtat gtatgcagca
      301 ggggccagta tgatcctggg tggtgtggcg cagatgctgg caccgaaagc cagaactccc
      361 cgtacacaga caacggataa cggcaaacaa aacacctatt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gcccgttctg tacggtgaaa tgcgcgtggg atcacgtgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagca
      601 tgacgaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aaggaccgat tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaaggtaat
      781 accaacatct ccggtgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttctgagacg gtgctgggta cggaagtgaa atacgacacg
      901 ccgatcaccc gcaccatcac gtcggcaaac atcgatcgtc tgcgctttac tttcggtgtg
      961 caggcactgc gggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     1021 gttcagatac agcgtaatgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     1081 accacgtcgc agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcactggtt
     1261 ggcgtgcagg tggactcgga gcagtttggc agtcagcagg tgagccgtaa ttatcatctt
     1321 cgcgggcgca ttctgcaggt gccgtcaaac tatgatccgg aaaaacgcac ttacagcggc
     1381 atctgggacg gaacgttaaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc acccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag taccggacgg ctttggcggc
     1561 acggagccgc gcatcacctg taatgcgtac ctgaccacgc agcgcaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accggccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc tttagcgccc tgaaagaccg ccataatgcc
     1801 gttgaggtga actggactga cccggacaac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattgcccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagtcggg ggcaggcaca ccgtgccggg ctgtggctga ttaaaacgga actgctggaa
     1981 acgcagaccg tggacttcag cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc
     2041 attgaaatct gtgatgatga ctatgcgggg atcagcatcg gtgggcgtgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggcacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt tcagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgg gttcctgacg gtgttgctga atacagcgtg
     2281 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgacgacg gcacgtatgc catcactgcc gtgcagcatg tgccggagaa agagggcatc
     2401 gtggataacg gggcgcactt tgacggtgac cagagcagca cggtgaatgg tgtcacgccg
     2461 ccagcggtgc agcacctgac cgccgaagtc tccgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacgacgga aaccacatac
     2641 cgcttcacgc agctggcgct ggggaactac aggctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc agcgccgtca
     2761 cagattgagc tgacacc
//
LOCUS       DAZKIF010000287         2765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZKIF010000287 DAZKIF010000000
VERSION     DAZKIF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.289"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2193
                     /locus_tag="ACHOTW_004912"
     CDS             <1..2193
                     /locus_tag="ACHOTW_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9846834.1"
                     /translation="RNLLKMDAFGCTSRGQAHRAGLWVIKTGLLETQTVDFTLGSQGL
                     RHTPGDIIEICDNDYAGTMTGGRVLSIDAASRTLTLDREVTLPETGAATVNLINGSGK
                     PVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLCRRLFRCVSVRENTDGTFAIT
                     AVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRV
                     VKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPA
                     TTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETAA
                     RYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGE
                     IGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKV
                     QVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPAN
                     GNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVN
                     ANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVY
                     DDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGV
                     IDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
     gene            2261..>2765
                     /locus_tag="ACHOTW_004913"
     CDS             2261..>2765
                     /locus_tag="ACHOTW_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9846835.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNP"
BASE COUNT          651 a          703 c          825 g          586 t
ORIGIN      
        1 cgcaacctgc tgaagatgga tgcgttcggc tgcaccagtc gcggtcaggc ccaccgtgcc
       61 gggctgtggg tgataaagac cggactgctg gaaacgcaga cggtggattt cacgcttggg
      121 tcacaggggc tgcgtcacac acccggtgac attattgaaa tctgtgataa cgactatgcc
      181 gggaccatga ccggcggacg tgtcctgtcc atcgatgccg ccagccgcac cctgacgctg
      241 gaccgtgagg tgacactgcc ggagaccggt gccgccacgg tgaacctgat taacggcagc
      301 ggtaagccgg tgagtgtgga catcaccgca caccccgcgc cggaccggat acaggtcagt
      361 accctgcctg atggtgtgga gacatacggg gtgtggggac tctccctgcc gtcactgtgc
      421 cgtcgcctgt tccgctgtgt ctccgtccgg gaaaacacgg acggcacctt tgccatcacg
      481 gcggtgcagc acgtaccgga aaaagaagcc atcgtggata acggtgcccg ctttgagccg
      541 cagtcaggtt ccctgaacag cgtcatccca ccggcagtac agcacctgac ggtggaggtg
      601 agtgcagctg acggccagta tctggcgcag gctaaatggg acacgccgcg ggtggtgaag
      661 ggcgtgcgct tcagtctgcg cctgaccagt ggtaagggaa cggatgccag actggtgacc
      721 accgccatca ccgcagacac ggagcaccgt ttcagcggcc tgccgctcgg ggaatacacc
      781 ctgacggtgc gggcgataaa cagctatggc cagcagggtg aacctgccac caccaccttc
      841 cggattgccg caccggcagc accgtcgcgg attgagctga cgccgggcta ttttcagata
      901 accgccacgc cgcatcttgc cgtttatgac ccgacggtac agtttgagtt ctggttctcg
      961 gaaaagcgga ttgcggatat caggcaggtt gaaaccgcag cccgctatct tggctcggcg
     1021 ctgtactgga tagctgccag tatcaatatc aaaccgggcc atgattatta tttttatatc
     1081 cgcagtgtga atactgttgg caaatcggca ttcgtggagg ctgtcggtcg ggcgagcgat
     1141 gatgcggaag gttacctgga ttttttcaaa ggagaaatcg ggaaaacaca tctggcccag
     1201 gagctgtgga cgcagattga taacggtcag cttgcgccgg acctggctga aatcaggacg
     1261 tccattacga atgtcagcaa tgaaatcacg cagaccgtca ataaaaaact ggaaaatcag
     1321 agtgcggcaa tccagcagat acagaaagtt caggttgata caaataataa cctgaacagc
     1381 atgtgggccg tgaaactgca gcagatgcag gacggacgcc tttatattgc gggtatcggt
     1441 gccggtattg agaatacgcc agcaggaatg cagagtcagg tgctgctggc ggcagacagg
     1501 attgcgatga ttaatcctgc gaatggcaac acaaagccga tgtttgttgg tcagggcgat
     1561 cagatattta tgaatgaagt gttcctgaaa tatctgacgg ctcccaccat taccagcggc
     1621 ggtaatcctc cggcattttc cctgacaccg gacgggcggc tgacggcgaa aaatgccgat
     1681 atcagcggta acgtgaatgc gaactccggg acgctcaaca acgtcacgat taacgagaac
     1741 tgtcgggttc tgggaaaatt gtccgcgaac cagattgaag gcgatctcgt taaaacagtg
     1801 ggcaaagctt tcccccggga ctcccgtgca ccggagcggt ggccatcagg aaccattacc
     1861 gtcagggttt atgacgatca gccgtttgac cggcagattg ttattccggc ggtggcattc
     1921 agcggcgcta aacatgagaa agagcatact gatatttact cctcatgccg tctgatagtg
     1981 cggaaaaacg gtgctgaaat ttataaccgt accgcgctgg ataatacgct gatttacagt
     2041 ggtgttattg atatgcctgc cggtcacggt cacatgacac tggagttttc ggtgtcagca
     2101 tggctggtaa ataactggta tcccacagca agtatcagcg atttgctggt tgtggtgatg
     2161 aagaaagcca ctgcaggcat cacgattagc tgaattttat aacccagata cgggcgccag
     2221 aaatggcgcc ttttttattg cagaaaagcg agaggtaatt atgcgtaaat tatgtgctgt
     2281 tattttgtcc gcagtagtct ggcaggtcgc cgctgctacg ccagcgagtg cagcagaaca
     2341 tcagtccacg ctgagcgcgg ggtatctcca tgcctcgacg aacgttcccg gtagtgatga
     2401 tctgaacggg attaacgtga aataccgtta tgagtttatg gacgcgctgg ggctgattac
     2461 gtccttcagt tatgccaatg ctgaggatga gcaaaaaacg cgctacagcg atacccgctg
     2521 gcatgaagat tccgtgcgta accgctggtt cagcgtgatg gcggggccgt ctgtacgcgt
     2581 gaatgaatgg ttcagcgcgt atgcgatggc gggtgtggct tacagccgtg tgtcgacttt
     2641 ctccggggat tatctccgcg taactgacaa caaggggaaa acgcacgatg tgctgaccgg
     2701 aagtgatgac ggtcgccaca gcaacacgtc tctggcgtgg ggagctggcg tgcagtttaa
     2761 cccga
//
LOCUS       DAZKIF010000288         2717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZKIF010000288 DAZKIF010000000
VERSION     DAZKIF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.290"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(197..928)
                     /gene="dnaQ"
                     /locus_tag="ACHOTW_004914"
     CDS             complement(197..928)
                     /gene="dnaQ"
                     /locus_tag="ACHOTW_004914"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP9846836.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            993..1460
                     /gene="rnhA"
                     /locus_tag="ACHOTW_004915"
     CDS             993..1460
                     /gene="rnhA"
                     /locus_tag="ACHOTW_004915"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP9846837.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1457..2179)
                     /locus_tag="ACHOTW_004916"
     CDS             complement(1457..2179)
                     /locus_tag="ACHOTW_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9846838.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2213..>2717
                     /gene="gloB"
                     /locus_tag="ACHOTW_004917"
     CDS             2213..>2717
                     /gene="gloB"
                     /locus_tag="ACHOTW_004917"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP9846839.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYT"
BASE COUNT          705 a          675 c          658 g          679 t
ORIGIN      
        1 gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg
       61 ctccgcgttg tgttccgttg ggaacgaggc gaatattacg gattgcctca cccttcgtca
      121 acggtttttc tcatcttttg aatcatttgc tgcaaaaatc gcccaagtcg ctatttttag
      181 cgccttttac atgtatttat gcccgccaga ggcagcttcc gccctttttc tgcaccagat
      241 cgagacgggc ttcatgtgct gcaagctctt catctgtcgc aaaaacaacg cgtaacttac
      301 ttgcctgacg tacaaggcgc tggattgttg cttcaccttg ttgctgttgt gtctctcctt
      361 ccatcgcaaa agccatcgac gtttgaccac cggtcatcgc cagataaact tccgcaagga
      421 tctgggcatc gagtaatgcc ccgtgcagcg ttcgtttact gttatctatt tcgtagcggg
      481 cacataacgc atcgaggctg ttgcgtttac cgggaaacat tttcctcgcc accgcaaggc
      541 tatcggtgac cttacagaag gtattagtct tcggaatatc gcgcttaagc aacgagaact
      601 cgtagtccat aaagccgata tcgaacgctg cgttatggat caccaactcc gcgccgcgga
      661 tatagtccat gaactcatcg gctacttcgg caaacgtggg cttatcgagc aaaaattcat
      721 cggcaatacc atgtacgcca aaggcttccg gatccaccag ccgatcgggt ttgagataaa
      781 catggaagtt attgcccgtc aggcgacggt tcaccacttc aacggcacca atctcaatga
      841 tcttgtggcc ttcatagtgc gcaccaatct ggttcatacc ggtggtttcg gtatcgagaa
      901 cgatctggcg tgtaattgca gtgctcatag cggtcattta tgtcagactt gtcattttac
      961 agttcgattc aattacagga agtctaccag agatgcttaa acaggtagaa attttcaccg
     1021 atggttcgtg tctgggtaat cctggacctg ggggttacgg cgctatttta cgctatcgcg
     1081 gacgcgagaa aacctttagc gcaggctaca cccgcaccac caacaaccgt atggagttga
     1141 tggccgctat tgtcgcgctg gaggcgttaa aagaacattg cgaagtcatt ttgagtaccg
     1201 acagccagta tgtccgccag ggtatcaccc agtggatcca taactggaaa aaacgtggct
     1261 ggaaaaccgc agacaaaaaa ccagtaaaaa atgtcgatct ctggcaacgt cttgatgctg
     1321 cattggggca gcatcaaatc aaatgggaat gggttaaagg ccatgccgga cacccggaaa
     1381 acgaacgctg tgatgaactg gctcgtgccg cggcgatgaa tcccacactg gaagatacgg
     1441 gctaccaagt cgaagtttaa gcctgtggtt tacgacattg ccgggtggct ccaaccgcct
     1501 ggcgaattcg tggcttgttt ttactctgtt tcatcggatt tagcgttaaa ggaatagtcc
     1561 gtttccgggc aacaataagt tgtaagcaac caagcgcagg aatatgcgca ttcaaaagtt
     1621 ttcctccgtg tttgttccac gggagaacgt ggaaacggct ggcgtgtagc acttcaaaat
     1681 tcaacaaaga gagccagtcc agttgccgca tcagagtaaa catccggctg ttatagggcg
     1741 aggttttgcg caataccggc acaagtttgc gtaatcccat taaactaatg ggattgaagc
     1801 cactaatgac cagccagcca tcatcaatca atacccgatc ggcttcacgc aataaacgat
     1861 gcggatcggt gcaccacggc aatgtatgtg ccagtagaca aacatcaacg gatttatcgg
     1921 caaaaggaag atgaagtggg tccgcctgca cctggacagg cattccttgc gcagaaacat
     1981 tcacttgatg agaaaccgcg cacgcttcgc aattgatttc tgcgcttaaa ttgccaatct
     2041 taagcagata aaaaccatac attttagtga accacgggtt gagctggcgc tccagcgcct
     2101 tgcgataaag ctctccccag ggcaaatcgc cccagcgatc aggagccacg acagtttgag
     2161 ggactcttgc cggtttcatc acaaccttcc gtttcacact gagaggtaat ctatgaatct
     2221 taacagtatt cccgcctttg atgacaatta catctgggtt ttgaatgatg aagcaggtcg
     2281 ctgcctgatt gtcgatcccg gagacgcaga gccagtatta aacgccattg ccgccaataa
     2341 ctggcaaccg gaggccatat ttctcaccca ccatcatcac gatcacgttg gcggcgtaaa
     2401 agaactggtg gaaaagtttc cgcaaattgt ggtgtatggt ccacaagaga cacaagataa
     2461 gggaacaaca caggtagtca aagatggcga aactgccttc gttttggggc atgaatttag
     2521 tgtaattgcc acgccgggtc acactttagg acatatctgt tacttcagta aaccttatct
     2581 attttgcggc gacacgctgt tttctggtgg gtgtggtcgg ttgtttgaag ggacagcatc
     2641 acaaatgtat caatcactta aaaagttaag tgcgttacct gacgatacat tggtatgttg
     2701 tgctcatgaa tatacgt
//
LOCUS       DAZKIF010000289         2687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZKIF010000289 DAZKIF010000000
VERSION     DAZKIF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.291"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..453
                     /locus_tag="ACHOTW_004918"
     CDS             <1..453
                     /locus_tag="ACHOTW_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9846840.1"
                     /translation="NLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEAD
                     PEQELRDRWVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGY
                     HGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            complement(470..724)
                     /locus_tag="ACHOTW_004919"
     CDS             complement(470..724)
                     /locus_tag="ACHOTW_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9846841.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            834..944
                     /locus_tag="ACHOTW_004920"
     CDS             834..944
                     /locus_tag="ACHOTW_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9846842.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            1247..2125
                     /locus_tag="ACHOTW_004921"
     CDS             1247..2125
                     /locus_tag="ACHOTW_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HFP9846843.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WMRLKNKELPPSPHHY"
     gene            2179..>2687
                     /locus_tag="ACHOTW_004922"
     CDS             2179..>2687
                     /locus_tag="ACHOTW_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9846844.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQA"
BASE COUNT          709 a          575 c          732 g          671 t
ORIGIN      
        1 aatctgttcg gtctggtcac cggaatggcg gaggacctgc agagcctggt gggggccacg
       61 gtggtccgcc gccgggtgta tgcccgtttt ctggatgcgg tgaattttgt ggcgggcaat
      121 ccggaagcgg acccggagca ggagctgaga gaccgctggg tggtggagca gatgtcagag
      181 ctgacggcca tgacagcctc gtttgtgctg gcaacaccga cggagacgga cggagcgctg
      241 tttcccggtc gcatcatgct ggcgaacacc tgtatgtggg attaccgggg agatgaatgc
      301 ggttatcacg gtcctgcggt ggcggatgag ttcgacaacc ccaccacgga tatccgtaag
      361 gacagatgca gcaagtgcat gcgcgggtgt gagatgcgcg gcatggtggc taattttggc
      421 ggtttccttt ccattaataa actttcgcag taatggatta tgcccaccat caggtgggtt
      481 ttttatcttc gccgtacatg ctgctgagag tcttaagaag agcctcccta aatttttcag
      541 attcttgttg agcaaaacct tctattgatc ttggtgattt ttcttcatca atagcagctt
      601 gcaagatcat aactatttct gaatttaatg agcgtccgtt tttcgatgct cttatggtca
      661 ggtcgcgctt gaggctatct ggcattctta cgctatatgg ggctatgtct ctaaccttcg
      721 tcatacttac accgtgataa tcacattgat atcacagtgt attcaaaaaa actttgactt
      781 gatatagtca cagtgatacg tttgtgattc ataataactc acgcggtggc gatatggaaa
      841 aagaagttag cagaattttg gtgagaatac ctcagtcgct aaaagatgcg attacaggaa
      901 aggccaaaga agaatgccgg tcgtttaact cggaagtgat gtgattgctg gacagcctga
      961 agagagaggg gataacggta tgagtaaaga atgttgtttt ttgcggcatt agcgaatcag
     1021 acgctgatca aacatacatt tactctaaag aaacaggtcg gatgctgtgt agtgactgcg
     1081 tgtttgacat cataagatac aagtatcttg gatgttctgc cagcattagc aatataggtg
     1141 aagtgtacga agggaaagat ataactgata gagcagaaag ttgaagcccc aactgctgta
     1201 acagtcaggg cttcgttatc aacaaatcgg cttaggaaat attgacatga aaagtatagc
     1261 aaaggcacaa aacgatttca ctatcttcaa attcggcgac agtgaaatcc gcgtcatcaa
     1321 caagtgcggt gagccgtggt ttgtagctaa agatgtttgt gatgctttag ctttgactaa
     1381 ctcacgcaag gcgcttactg cacttgatga cgatgaaaag ggagtaactt taagttacac
     1441 ccttggtggt gagcagaatc taagcattgt gagcgaatca ggtatgtata cattggttct
     1501 gcgctgccgc gatgcagtca ataaaggttc agtcccgcac aaattccgca agtgggtaac
     1561 agcagaagtt ctgccttcaa ttcgcaaaca tggcgagtat gtaaaaggaa agaaaaccac
     1621 tgttgaggaa agaacaccgc tacgcgatgc agtaaacatg ctggtaggaa agaaaggact
     1681 tcgcaatgac gatgcataca atatggttca tcagcgtttt ggtattgaca gcattgatga
     1741 gctttcaatt gaacaaatcc cgcttgccgt agagtacatc cacagggtag tgcttgaagg
     1801 tgaattcatt ggcaaacaag agaagagcac caacgagctt tccgcaaaag aagcaaacag
     1861 ccttgtatgg ctatgggatt atgccaaccg ctcacaggca ttattccgcg aactgtatcc
     1921 ggcgctaaaa caaattcaat cgaactattc cggcagatgc tacgactacg gtcatgagtt
     1981 ctcgtatgtt attggaatgg cgagagacgt tttaattaat cacacacgag atgttgatat
     2041 caatgagcca gacggaccaa cgaatctttc cgcatggatg agacttaaga ataaagaatt
     2101 acctccttca ccgcatcact actaacagat tgccaacgaa atgacccagc ttcggctggg
     2161 tttttatcag gagttctcat gctctatagc aatatattgg cgcacgcccg gcgatgtgca
     2221 ccagcggagt cgtgcggctt cgtggtgaga acgccggaag gggaaagata ttttccctgc
     2281 gtgaatatct ccggtgagcc ggaagattat ttccggatgt cgccggagga ctggctgcgg
     2341 gcagaaatgc agggggagat tgtggcactg gtccacagcc accccggtgg tctgccctgg
     2401 ctgagcgagg ccgaccggcg gctgcagata aaaagtgcac tgtcctggtg gctggtctgc
     2461 cggggggaaa ttcataaatt ccgctgtgtg ccacatctga caggacggcg ctttgagcac
     2521 ggggtgacgg actgttacac gctgttccgg gatgcctacc atctggcggg aattgatatg
     2581 ccggattttg agcgtgagga tgactggtgg cgcaacggtc agaaccttta cctggacaat
     2641 atggcggtca ccggctttta ccgggtgccc ctgtcctctg cacaggc
//
LOCUS       DAZKIF010000290         2669 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZKIF010000290 DAZKIF010000000
VERSION     DAZKIF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.292"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(27..1208)
                     /locus_tag="ACHOTW_004923"
     CDS             complement(27..1208)
                     /locus_tag="ACHOTW_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9846845.1"
                     /translation="MPLNDMQIRRAKPEAKAYTFGDGLGLSLLIEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLAMQTESENAFEK
                     IAREWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVFRYAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPDFLRA
                     LEGYSGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSSQAVDLLNELKIMTGNYRYVFPGRNDPNRPMSEASINQAIKRIGYGGKVTGHGFRH
                     TLSTILHEQGFESAWIEIQLAHVDKNSIRGTYNHAQYFSGRKSMMDWYSNLIFERLKR
                     S"
     gene            1695..2129
                     /locus_tag="ACHOTW_004924"
                     /pseudo
     CDS             1695..2129
                     /locus_tag="ACHOTW_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2171..>2669)
                     /locus_tag="ACHOTW_004925"
     CDS             complement(2171..>2669)
                     /locus_tag="ACHOTW_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9846846.1"
                     /translation="NFADFSLIKGLISWANHCAEYIEIFDESIAFTCLSAFSSEKQFG
                     VFLFGCLKSTGAKVKTIIHTDLSAPWRLKDISSRLYLSESLLKRKLKEEGVSFSKIIL
                     DERMQMAEYLLSTRCYPISKVAKVCGYASVSYFTYVFRRYFGVSPSQYSQRSSESKIL
                     THQGI"
BASE COUNT          780 a          620 c          543 g          726 t
ORIGIN      
        1 atttccgaaa gtggttgaga cacaaattaa ctccttttta gtctttcaaa tatcaaatta
       61 ctgtaccagt ccatcataga cttccttcca ctaaaatatt gagcatggtt ataagtcccc
      121 ctaatagaat ttttatctac atgagccaac tggatttcaa tccaagcact ctcaaaacct
      181 tgctcatgca ggattgtaga aagggtatga cgaaaaccat gtccagtgac ttttcctcca
      241 tacccaatac gcttaatggc ttgatttata ctcgcttcgc tcattggcct attcggatcg
      301 ttccgccctg gaaaaacata acgatagttc cctgtcatga tcttgagttc attgagtaaa
      361 tctaccgctt gagatgataa gggcacaaga tgtggcctac gcattttcat cctttcagca
      421 ggaatttccc aaatagcgtt atccagatca aattcttgcc ataatgccgc gcgtaattcg
      481 atggttctca cacccgtaat catcagtaat ttcgtggcta tctggacaag cttactcccg
      541 gagtaaccct ctaaggcacg tagaaaatca ggtatctcat cagcttttag gaatgggaaa
      601 tgattggatt ggtgtacttc gagagcgctg gagagatcag ccgcaggatt gtactccgcc
      661 ctacccgttg caattgcgta gcgaaacact tcggagcaac gctgccgcac tttgcgcatt
      721 ttctccaacg caccacgttt ctcaatttta cgcagaacgt tcagcagctc tagcggttta
      781 atctctccca caggccttgt tccgacataa ggaaaaatgt cgttcttaaa cgcttccatg
      841 atgtctgatg catatcccgc cgaccattta gcagatttaa gttgatgcca ctctctggct
      901 atcttttcga aggcgttctc tgactctgtt tgcatagcta gcttttgctc ttttcgaacc
      961 tcactagggt tctttccttc tgccacaagt tttcgagctt catcacgacg ggaacgagca
     1021 tcggcaaggg tgatcgttgg gtaaacacca agcgagatca ttttgggttt gccggcatag
     1081 cgatagcgga accgccaact cttgcttcca ttaggttcta taagtaatga caaccctagc
     1141 ccatctccaa atgtataggc tttagcttca ggcttagcgc ggcgaatctg catatcgttt
     1201 aaaggcatgt gtataggaat ccagaccgaa caggaacata tacacaatcc tatacacatt
     1261 cagtaccgga ttctactgga tggttacgga ctactatgga cttaaaagaa taaaaaatgt
     1321 tataaatcag agattttatg gactaatacg gacgtttgag gaagttgaga tggtgccgat
     1381 aataggagtc gaacctacga ccttcgcatt acgaattata agaatccgct tctaattcaa
     1441 agcattaccc catcaacact gcgctcacac gtcccaccac atcaaaacat gtaaagcctt
     1501 gcaagccatt gcgaggcctt atgtgtctca gttttgtccc accttgtatt acgacttgca
     1561 tagccaatga agataaacgt gacgacaaac ggcgcagcag tcttcttttc tttcacgact
     1621 tctccagcat gcataccttt ctgccatgac tgaagtgaat gcctgttatg agcaaggatt
     1681 ggcccagatg aatcgccctg gactgttgcg atcgtgaagc catagactgg gccgcaagca
     1741 cgggaggtta cgataaagcg acagtgcagg atgtcatgtt aggagcgata gaaaaacgct
     1801 ttggcgataa agtaccggaa cagtccatcc agtggctgac agataacggt tcagcgtata
     1861 gagcgcatga aacacggcag ttcgccagag agttgaatct ggagccctgc acaacggcaa
     1921 tcagcagtcc tcagagtaat ggaattgcag aaaggttcgt gaaaacgatg aaggaagact
     1981 acatcgcgtt catgccgaaa ccgaatgtaa gaacggcatt gcataatctt gcagtggcga
     2041 tcgaacatta caatgaaaac catccgcaca gtgcgttggg ttatcgctct ccgcgagaat
     2101 atcgacgtca gcgggtaacg ttaacttaag ataccccccc tgtctggaaa taagggggca
     2161 agaacaatga tcagattccc tggtgagtaa gaattttact ttctgaactc ctctgagagt
     2221 attgacttgg agaaacacca aaataacgtc taaatacata agtaaagtat gagacactgg
     2281 cataaccaca gaccttagct actttactaa taggataaca acgagtgctg agtaaatatt
     2341 cagccatttg catcctctca tcaagtatga tcttactgaa tgataccccc tcttctttca
     2401 atttcctctt tagtaaactt tcgctgagat ataatcttga tgatatatcc ttaagacgcc
     2461 atggtgcaga taaatccgta tgaataatcg ttttaacttt agcccctgtg cttttcaaac
     2521 atccaaacag aaatactcca aattgttttt cagaagaaaa tgcagagaga catgtaaaag
     2581 caatagactc atcaaaaatt tctatgtatt cagcacagtg attagcccaa ctaattaatc
     2641 ctttaattaa actgaagtca gcaaagttt
//
LOCUS       DAZKIF010000291         2649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZKIF010000291 DAZKIF010000000
VERSION     DAZKIF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.293"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..1141)
                     /locus_tag="ACHOTW_004926"
                     /pseudo
     CDS             complement(1..1141)
                     /locus_tag="ACHOTW_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
     gene            complement(1382..2428)
                     /locus_tag="ACHOTW_004927"
     CDS             complement(1382..2428)
                     /locus_tag="ACHOTW_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP9846847.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
BASE COUNT          715 a          663 c          553 g          718 t
ORIGIN      
        1 ctctccggaa tcatcaatgg tttgactttc agtatcatga agtttaacaa caaatcaatt
       61 ctgtttctga atccaatccg cctgtcagaa aggtaatgaa tataagccat caggtcggtt
      121 gttttttgtg aggaaaatcg gtatttgtga gaaaaaagct cctccggtcg aagattctga
      181 ctctgccatt gctgcaggta tttatactca atattcagct cattaatttc tgcttttaac
      241 gaagcgacct ggttaatggc attcaggtgt tttttcactt gttgaagtac atcatcaatc
      301 gcatttgcag acggtgagtc aggtaagact tcctcagaac gcgaaatcga cgttgaaaaa
      361 aactgttgcc tgttctccgt actccccagt cgtgccatca catatcccag ttgctgtttg
      421 tccatctttt cataaacatt actgacagct gagttattat tggacaaaat tgccactgtt
      481 ttattctgaa ttaatatatt agcaacaata ttgagtatag tctgtgtttt acctgttcca
      541 ggtggtcctt caataactga aatctgtgag gaaaatgccc gttcgacagc cagcaactga
      601 ctttcattaa gaccaaacgg aaaaatcaaa ccccatggcg aatcacgttt ttactctgtc
      661 ctgaacagta tgcatgcaag gcggtatccg gatgaggaac tattttcttt atctgcctaa
      721 ggatattctc atcgatattt ctgttattac cacgggcata aaaaatacgt tcattagcca
      781 ccctacacaa gtaattaaac acggcatgct ctgtaaatgc tgaacactgt tgaaagtcaa
      841 ggccggtcgt tttcatgatg tagggtcgtt caccctcgcg gtaataaaca gcagcatatt
      901 tatcaccata tataataacc agttcagccc gggatgttac agcgttccct ttcctttcga
      961 ggaaaacatt tcgcaaattt acagttggcg ttatctgaca tacagaaagc ggacgagtat
     1021 attttctccc gctgcgaaaa tgacaggtca gcataacctc tctgtcgtca tttccagccc
     1081 acagcgtcca gtcggtaatt ttatgagtct tattttcacc gtcaacacaa atcgtgacca
     1141 tattcttact cagaaattat catttaactt acggaaaatt gttaaggcac tcccgatatt
     1201 agctccggag gattttccac aactccggga ctgttaacat atttctgcct gccatatata
     1261 cattgaattt agtcataaca gtcaatacca gaattacagg aatgcgatgc cgatacgctg
     1321 gttattgaat actggtagcc cctgagcact aattcatcac gatactctgt atttactgtt
     1381 ttcactgctt tttacaacca ttcccccagg taaaatgctg ctcctccggt gatggctgtg
     1441 tcagaaactg ccatcccgga atagccacgc tgaccgccag caatgccgca cagacaccag
     1501 ccacaaacgg tttcagaaac gtgggcgggg atttctgaag ggagggtgac tgctccgcct
     1561 gcactttccc cttgcgatta tcaaggctca gtgcatccag acgcaccgcg ccatttgccg
     1621 caagcagccc ttcccggtta tccacgcttc ccggcagact cagcgaaaaa tcccctgttc
     1681 ccgtctgaat cagactgcct ttacgatttg acaggctttg tgccgatatc tgcagtgtgt
     1741 cggcatttat cgtaccgcca ttactgctga actgcccggc agtccgggcg gaaagcgtat
     1801 cggcactcag ttgcgcattc tgtgtagaca gatttccact ggttgcattc agctcagtat
     1861 gttgtccctg tatccggctg ttacctaaat ccagcccctg ctcaatgtcc cgtaaatcca
     1921 gcagagaacc ttcgtgtgcc gggaatgagg aatacaactg aatatagtca tcactgtccg
     1981 gtgtcagacg cacatgtcgc acaacaccgg gaataataac cagccggagg ataccgcttt
     2041 ttaaatcctg cgaaggtgcc agaacacggg tggtgacata accatgatcg accagacggt
     2101 tctgcaatgt actcatcagc agattaattc ctttcgcccc caggcaatgc ccgaccgccc
     2161 cgttggcgat tttttgtaaa ggcagccagt gtggtaacgc atcagccccc ttcagttccg
     2221 tctgtttaat ctgaaaacag ggcgtttcaa caggaaaatt aatcttatgg gcaaaagagc
     2281 caggtgcaga aaggcgaaca tcaggggctg aaggcgtcag ttgttgctca agggcttcct
     2341 gacgctgctg ctgaatgata aatgcttcat ctccgctacg ccggacatcc gctgcctgaa
     2401 gagataaagg cgataataaa gcagacatca gtgtcagtga cagcaaggac ggacagcgcc
     2461 ggaataagct ttgttcctca gtcctctcgt tcctgcaaca taattttcca gtcaaacgaa
     2521 aaacctttta ttcatgtcat accaacaatc accatgaacc gccccgggaa tcctggagac
     2581 taaactccct gagaaagagg taaacaggat gactaaaaat acacgttttt cccccgaggt
     2641 ccgtcaacg
//
LOCUS       DAZKIF010000292         2607 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZKIF010000292 DAZKIF010000000
VERSION     DAZKIF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.294"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..392)
                     /locus_tag="ACHOTW_004928"
     CDS             complement(<1..392)
                     /locus_tag="ACHOTW_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9846848.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSG"
     gene            complement(376..699)
                     /locus_tag="ACHOTW_004929"
     CDS             complement(376..699)
                     /locus_tag="ACHOTW_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9846849.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            824..1300
                     /gene="racR"
                     /locus_tag="ACHOTW_004930"
                     /gene_synonym="rarC"
     CDS             824..1300
                     /gene="racR"
                     /locus_tag="ACHOTW_004930"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFP9846850.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            1616..1768
                     /locus_tag="ACHOTW_004931"
     CDS             1616..1768
                     /locus_tag="ACHOTW_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9846851.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(1883..2398)
                     /locus_tag="ACHOTW_004932"
     CDS             complement(1883..2398)
                     /locus_tag="ACHOTW_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846852.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            2522..2607
                     /locus_tag="ACHOTW_004933"
                     /pseudo
     CDS             2522..2607
                     /locus_tag="ACHOTW_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          738 a          633 c          561 g          675 t
ORIGIN      
        1 ccactgttag tcagggtgcc ccaggcgatc gccgctgcga caaaatcatc cacatctttc
       61 accagccgat ccctccgttc gacgatctca cggtaatatt cagagctgtg actgcgcata
      121 cgggccacca gcagaggcgg cattgccttt tcgatcgccg gtaacagagc ctgcattttt
      181 tcaacagcat caggggtgtc tttatccagc caacggaaaa ttttctgggt attacgagcc
      241 agggcttccg gatggctgtc gtcgtacagt tccgggaacg tcatccccag ttcgaaataa
      301 gtccgggcta tttcagctgc aggaactttc tcaccatcag gatatgccca ggcattcatc
      361 gccatgcgga tgtgctcatg tttgattttc atgaatcatt tgcctcttga tgcttcgggt
      421 atgatcgttt tcgtcatttg gttgcttcat cgacatattc tgcgaataac atgacgagcg
      481 tcgtaagtat gtccaatcaa catcaggacg aagttcttca cacaggacac caccttttgt
      541 tgctcgttca atcgcaggac atctctcagc aggcaactga cgtacacctt tgatccattg
      601 atttacgctt ggaggagata cacctaaaag cctagccatt gctgattgcc caccgacaac
      661 agcacaagct cgtttgaatg aatagttatc ttttttcatc gaatgaactc caaaaaacac
      721 acaataatat taggcttagc ctaatgcaat tgtcaatagg ctatgcctaa cgcttcgaga
      781 gtaggcattg cctaatgcga tgcgcatagg agactattaa gcaatgctta gtggtaaaga
      841 cttaggccga gcgatagagc aggccattaa caaaaaaatt gcatcaggag ccgtcaaatc
      901 aaaggcggaa atcgcacgtc atttcaaagt ccaaccacca tcaatccatg actggattaa
      961 gaaaggttcg ataagtaaag acaaacttcc agaactatgg cgtttctttt ctgatgtggt
     1021 tggtccagag cattgggggc ttaacgaata ccccatacca accccatcca cttcagatac
     1081 aaaaagtgaa cttttagaca taaacagcct ttatcaagcc gcctctgatg aaaaaagagc
     1141 aattgtggct ttcctcttat ctggaaatgc tacggagcct agttgggttg atcatgacgt
     1201 tcgtgcctac attgccgcaa tggaaatgaa ggtagctaac tatctgaaaa atcaagaatc
     1261 aaaacggaaa agccagaaca tcaccaagac aggaacttaa acttatatgg tccgacggga
     1321 aattcctagt tcccgttagt taactcctac tacctctccc acaaaccatc acctattagg
     1381 tcgcacccaa atcattaggc gtagcctatt gacaagcaat taggcatttc ctatagtttt
     1441 cccataccaa cccatcccgt cccacacaat acagggcaat acctagagtt acccggcagt
     1501 ggtcagggat taagtagcca gcccgaggcg taagaacatg acggcagggt tcaactttaa
     1561 ctatgcagca ggtttttgtt ccgctacccc ggcgttaagg ggatatgagg tcagcatgga
     1621 tactcttgat cttggcaaca acgaatctct ggtgtgcggc gtgtttccca accaggacgg
     1681 cacgttcacc gcgatgacgt ataccaaaag caaaacgttt aaaaccgaat ctggcgcgcg
     1741 tcgctggtta gcaagaaact ctgactaatg aaatctggta attaaggaat cctccacggg
     1801 gggagagtgg tgcacacgcg ccggacacaa gcaagcatcc ggcatgctct ttaacaatct
     1861 ggatatccat aacagtaaaa atctacagat tgccgttcag ttttctggcc aactcttcaa
     1921 tagatggagg tgatacgtaa tccggatttt tattcataag aaactgattt tcacagtaca
     1981 ggcacctgct tttatgaaaa aattcatatt ctctaaccgg gaatggctga agtatcgata
     2041 ctatcttttg tccaaaacat tttggacaaa gatgcatgat tatgctgccg ccgttcacgg
     2101 ttacctcctt cgagtacaca aaagttcccg actcaagttg gctaaggata taaccttccg
     2161 tctgagcctc aaagttttca aattctgcaa ttttagcttt gagagaagca tttatttctt
     2221 gataagtgcc caccagctca acgagagaca cgcattcgcg ctgaatagat gcaagctttg
     2281 agttcagttc accaatagcc gcatttactt cagcttgagt ttttgcctcg ttcattagtt
     2341 ttgcaatctg ggcggtttca cgaatagccg tcattgctgc cgttaattca gcgatcacat
     2401 tcaatactct tattgttgtt ggggatatcc agattaacaa gatccttgtt gttggggaat
     2461 aacggggacc acctcgcctg acgtggtaaa aagcaggcac acaacacgaa agcacacggc
     2521 gagggacacc attaaatggt ggcttaagca atcacgcgaa gcgcaatctg ccattatgac
     2581 cgatgaaatc ccgttagatg atgcact
//
LOCUS       DAZKIF010000293         2559 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZKIF010000293 DAZKIF010000000
VERSION     DAZKIF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.295"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1470)
                     /locus_tag="ACHOTW_004934"
     CDS             complement(<1..1470)
                     /locus_tag="ACHOTW_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP9846853.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASR"
     gene            complement(1518..1727)
                     /locus_tag="ACHOTW_004935"
     CDS             complement(1518..1727)
                     /locus_tag="ACHOTW_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP9846854.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(1823..2197)
                     /locus_tag="ACHOTW_004936"
     CDS             complement(1823..2197)
                     /locus_tag="ACHOTW_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP9846855.1"
                     /translation="MKKDLKTLALARLSGVRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(2212..>2559)
                     /locus_tag="ACHOTW_004937"
     CDS             complement(2212..>2559)
                     /locus_tag="ACHOTW_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP9846856.1"
                     /translation="TNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVNTGALLTM
                     SVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSAEKAQSVT
                     SVECTVTVSAAAG"
BASE COUNT          505 a          873 c          615 g          566 t
ORIGIN      
        1 ccgactggcc tgttcatcaa tcagccggtt ctgctctgtc agcgccgccc gtgttcgtcc
       61 ttcattatct gaggcttcag gcagagacat tgacggcgtt ttatgcgcga tttcatctat
      121 cgtcagtgca tactggcgcg cagactccct ggcctgctcc tgattctggt acagcgtgta
      181 ccatgctgca gcccccagca tcaccagtcc gggtacgcca ccaaccagcc ccagcgcacc
      241 gctcatcaga cgtgagccca ccgccgttgt actgttcagc gcattctggg cggcgcttct
      301 ggcagcaata tttctgttca ggcgttcctg tgtggccgcc agacgggcct ctgcagcaat
      361 ctgcatctcc gtcccgcggg ctgccgccac ggcctgctga gcacggtaca cggctgccct
      421 tgcccgcgcc gtggcaatct gcgttcccct gaactgtgct tccgccagtg caacttcatt
      481 acgtgcagcc gtcacaagtc ctgccgtggc agacatcgct ccggaggcca tattgccaaa
      541 gtaccgggca accccgacgg caaccagcgc gcccacggct gttgccacat tatcaatctg
      601 tccggcaaca ccgttcagca tgccggagag cgtttttgtc accccgctgg cctcattcgc
      661 accgcccacc caggccataa aggcgttttc cacctttgtg atactactgg aaaccgtttc
      721 cggcatggcc gcatattcat cacgtaatat ccccagctgg ctgattaacg cggggaccac
      781 tttatccgct gtcagttttc cgtcatccgc cattgccttc agatctttac gggccacgcc
      841 catacccgca gccagtgcac gtacgatccg gtcaccactt tcattgaccg aattaaactc
      901 ctcaccacgc aatacaccct gcgccagcgc ctgactgaac tgggtgatca ccgagcccgc
      961 ctctgccgta ctggcaccgg agattttcag ccctgtcgaa atggcctccg tcaccttcag
     1021 cacatcatca gcactgtaac catattcacg catcgaggca gccgaacggg caaacagggc
     1081 cgcattatcc gaaaatgcgg tgcctgtccg ctggctgata tccatcagca ctttctgtga
     1141 tgacgcaaat tcatcggatg actgtgatgc ctgtttcaga cgggcattca cggagctcca
     1201 ttcatccgcc agcgaaatca ggtgtccggt ggcaaaggca ccggcaaacg caccggtcat
     1261 tccgacagcc gaagcgcgga tttccgtcaa ctggctgtgc agctcagcca gagcccggcg
     1321 ctgctccctg gctgccgcag cagcctgacg tccgccattc tgcagggtcc ggtaatattc
     1381 actgcccata cgggacgccc gctggatctc cgactggaat gactgtgaat ttgccgaaat
     1441 tttgataatc agttcacgta acgtcgccat tcacctttct ccgggcgtaa aaaaaccgcc
     1501 tcagcggttc tcatcattca tgactgtgct gcaaggctca gcgcgtcttc cagcgccgca
     1561 aacggatcca cctccggctt atcctcatcc tcgccccagc agagcatggc gtccttcagt
     1621 gcaacattca tcccctgtgc cccgaaaacc gctttcacga tctgtgcatt acggatatcc
     1681 ccgcgctcat cacccagcgg ggatatcctg tcgaactcca tccacatcat cgcctcgctc
     1741 gcactcaggc tgtggcgcag ttcggataag gtgcgcccca gacggagcgc aagtcgcatc
     1801 agaaagcgaa tttccgggcg ggctactttt ttctggccga ctctgcatca gcgatcagtt
     1861 ccagtgcctg acgcagcaac cgggcatgta ccggaccata gacggccagc acctgctcac
     1921 ggtcgtccgg agtgaacacc cgctgcaggt ccgtatcaca caggacatcg cagaacagcg
     1981 tcacatccgc ttccaggtta cggcgggttt tcgccaccac cgacagggta tcgtcatcct
     2041 ctccatcacc attgagcact tcctgccaca gataccaggc ctctgccgaa ggctcccgca
     2101 gcaccacgct gacattaccc cattccggca ccttcaccgt tttatgacga acccctgaca
     2161 gtctggccag cgccagcgtt ttcagatcct ttttcatgat gacccatccc cttatccggc
     2221 ggctgcgctc actgtcacgg tgcattcaac agacgtcaca ctctgtgctt tctctgccga
     2281 atcggtcacc acacaggtat atttccccgc atcagcggac tgcgcacctg gcttactgaa
     2341 ggtgtctgtc gtctgcccgt caaccggctg accatccttc ttccaggcgt atttatacgg
     2401 cggcgttccc ccgttggcac tgactgacat tgtcagcagc gcaccggtat tcacggtaag
     2461 tgtcttatcc agatttttca caaacgccag cggtaccaca aaggacaccg gtttgccttt
     2521 cagacgcagt gagaacgttg cagccaccac gccgttggt
//
LOCUS       DAZKIF010000294         2537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZKIF010000294 DAZKIF010000000
VERSION     DAZKIF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.296"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            5..2152
                     /locus_tag="ACHOTW_004938"
     CDS             5..2152
                     /locus_tag="ACHOTW_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9846857.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIN
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
BASE COUNT          744 a          561 c          567 g          665 t
ORIGIN      
        1 tgaaatgtta tataatatac cttgtcgaat ttatatcctt tccactctgt cattatgcat
       61 ttctgggata gtttctactg caaccgcaac ttcttcagaa acaaaaatca gcaacgaaga
      121 gacgctcgtc gtgaccacga atcgttcggc aagcaacctt tgggaaagcc cggcgactat
      181 acaggttatt gaccaacaaa cattgcagaa ctccaccaat gcctccatag ccgataattt
      241 gcaggacatc cccggagtag agataacaga caactccttg gcaggccgta aacaaatccg
      301 cattcgtggc gaagcatcct cccgtgtttt aattctcatt gatggtcagg aggtaactta
      361 tcagcgcgcc ggagataatt atggtgtggg actgttgata gatgagtctg cgctggagcg
      421 tgttgaggta gtgaaaggtc catattccgt actgtacggt tcacaggcaa ttggcggtat
      481 tgttaacttc atcaccaaaa agggaggtga caaacttgca tctggagttg tgaaagctgt
      541 ttataattcc gcaacagcag gctgggaaga atcaatcgcg gtccagggga gcatcggtgg
      601 atttgattat cgcatcaacg gtagttattc tgatcagggc aatcgtgata cgccggatgg
      661 acgtctgccg aataccaact atcgtaacaa tagtcagggt gtatggttgg gttataactc
      721 cggaaaccat cgttttggcc tctcgcttga tcgctacaga ctcgcgacac aaacttacta
      781 tgaggatcca gacggaagtt atgaggcatt tagtgtcaaa atacctaaac ttgaacgaga
      841 gaaagttggg gtattctatg acacagacgt ggacggtgac tatctaaaaa aaattcattt
      901 cgacgcgtat gagcagacca tccagcgcca atttgccaac gaagtaaaaa cgacacagcc
      961 tgttcccagt ccgatgattc aggctctgac cgttcataac aagactgaca cccatgataa
     1021 gcaatacact caggcggtca cattgcagag tcacttttcg ctgcctgcta ataatgaact
     1081 tgttaccggt gcacagtaca aacaagatag ggtcagccaa aggtccggtg gcatgacctc
     1141 aagcaaatct ctgaccggct tcattaataa ggaaacacga actcgctcct attatgagtc
     1201 agagcaaagt acagtctcac tattcgcaca aaatgactgg caattcgccg atcactggac
     1261 atggacaatg ggagttcgcc aatactggct ttcttcaaag ttgacgcgtg gtgacggagt
     1321 atcatatacc gcaggcatta taagcgatac ctctcttgcc agagagtctg cgagtgatca
     1381 cgaaatggta acatctacaa gcctgcgcta ttcaggtttc gataacctgg agttacgcgc
     1441 tgcgttcgcg caaggctacg tatttcccac actctcccag ctttttatgc agacatctgc
     1501 gggcggcagt gtcacatacg gaaatcctga tcttaaggct gaacactcca ataactttga
     1561 attaggtgca cgatataatg gtaatcagtg gctgattgac agcgcagttt actactcaga
     1621 agctaaagat tatattgcaa gtctgatctg tgatggcagt atagtttgca atggtaacac
     1681 caactcctcc cgtagtagct actattatta tgacaatatt gatcgggcaa aaacatgggg
     1741 actggaaata agcgcggaat ataatggctg ggttttctcg ccatatatca gtggcaattt
     1801 aattcgtcgg caatatgaaa cttcaacatt aaaaacaact aatacaggtg aaccagcgat
     1861 aaacggacgt atagggctga aacatactct tgtgatgggt caggccaaca taatctctga
     1921 tgtttttatt cgtgctgcct ctagtgcaaa agatgacagt aacggtaccg aaacaaatgt
     1981 tccgggctgg gccactctca actttgcagt aaatacagaa ttcggtaacg aggatcagta
     2041 ccggattaac ctggcactca ataacctgac agacaaacgc taccgtacag cacatgaaac
     2101 tattcctgca gcaggtttta atgcagctat aggttttgta tggaatttct gatgacaaaa
     2161 acaatcgtta tggaattctt tgtcctgatc tgcccgcttt gcgggcaggc taacgaacgt
     2221 attgtgtttg ttgaaggttt ttcgacgttg ttttccatct gtgggtgaat atttattctt
     2281 cccgccagca tgactctata ctcattgcta cgacaatgac ttataaagct ggttactgaa
     2341 cctatacctt tgtaattcaa aagtgatatc agttcacaac gcaatactat ttttagatta
     2401 tttactctca cccagatact gaatcaatgg ataatacctt ttgatttatc ccgacgcgga
     2461 agaaggtggt gaaccgcccc ggaaatcctg gagactaaac tccctgagaa agaggtaaac
     2521 aggatgacta aaaatac
//
LOCUS       DAZKIF010000295         2532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZKIF010000295 DAZKIF010000000
VERSION     DAZKIF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.297"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            206..903
                     /locus_tag="ACHOTW_004939"
     CDS             join(206..455,455..903)
                     /locus_tag="ACHOTW_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HFP9846858.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            920..1180
                     /locus_tag="ACHOTW_004940"
                     /pseudo
     CDS             920..1180
                     /locus_tag="ACHOTW_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(1322..2446)
                     /locus_tag="ACHOTW_004941"
     CDS             complement(1322..2446)
                     /locus_tag="ACHOTW_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFP9846859.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          722 a          578 c          524 g          708 t
ORIGIN      
        1 cgttcattct atggttacct cttcctgtta tatagaagaa ttttaaacga caagtaaggg
       61 aatataaatg aaaataacaa aaaataaaaa tgcaactata aaaaacagtc atcagtcggc
      121 tgcttttcaa tgtttacctt atcattttct ggtaatgctg ccaacttact gatttagtgt
      181 atgatggtga ttttaaggtg cttgcgtggc ttccatttcc atcagatgtc cttcctgctc
      241 cgctactgaa ggcgtggtgc gtaacggcaa aagcactgcc ggacatcagc gctatctctg
      301 ctctccttgc cgtaaaacat ggcaactaca gttcacttac accgcctctc agcccggtaa
      361 gcaccagaaa atcattgata tggccatgaa tggcgtcgga tgtcgcgcca gtgcacgcat
      421 tatgggcgtt ggcctcaaca cggttttacg tcacttaaaa aactcaggcc gcagtcggta
      481 acctcgcgca tacaaccggg cagtgatgtg attgtctgcg ctgaaatgga cgaacagtgg
      541 ggctacgtcg gtgctaaatc acgtcagcgc tggctgtttt acgcgtatga caggatacgg
      601 aggacggttg tggcgcacgt cttcggtgaa cgcactctgg ccacactgga gcgtcttctg
      661 agcctgctgt cggcctttga ggtcgtggta tggatgacgg atggctggcc gctgtatgaa
      721 tcacgcctga agggaaagct gcacgttatc agcaagcgtt acactcagcg cattgagcga
      781 cataatctga atctgagaca acatctggca aggctgggac ggaagtcact gtcgttctca
      841 aaatcggtgg agctgcatga caaggtcatc gggcattatc tgaacataaa acactatcag
      901 taagttggag tcattacctg agaataatac ctcctgggta agtgagcaga tactttcagc
      961 gcctccgaaa gatgtaaata ttcgacttgg agtcggttct ttagaaggta caaccgtctc
     1021 acatgttcag cgattgcatc attcgttaat cgcagctggt ttggaaagta acctcactgt
     1081 ctataccggt ggtcacgatt atgcctggtg gcgcggagca attattgatg aattagcaaa
     1141 ttataattgc aggaaggtat caaataataa ctctgtgtaa aatatcgttt tatcgtcaga
     1201 taactctttc tgcaatatta ttatgggaga ctatctcatt tatttagtga tgtccttcat
     1261 ttatgtgatg aaggacatta tattactcat aacatttctc gttgaaaatt tcagcaatac
     1321 gctacaattg tttcccgttg ttttttaatt ttctcatcaa aacctctgca atctctgcag
     1381 gactgggttg ttcagccatt tcagcggcta cctgatctga acagaagcgt agtgaatcat
     1441 cataaagaag gcgatttacc aaatcactgg tcagtccatg cttgtccgga ataatcccac
     1501 acccacgctt cgcaacaatt tctgcattaa cagagcgatc tgcaccttcg ccaaacacaa
     1561 tctgtggtat tccactatac agcgctgtca gggtattacc tgcgccaccg tgatgaataa
     1621 atccatcagc tccattaagg aatacaccca taggtatcca gtcaaccaga cgtacatttg
     1681 atggtagttt tcgtaaacca gtacgagcat ttattgcaag ttgcaaaatg atatcagcat
     1741 caacttcatt tgcagaatcc ataacccatg aaatcagctc cagaccatca accattggtt
     1801 ttacagtgcc aaggctgata agtaaacgtt tccgatcaga atccctgtcc caccatgttt
     1861 cctttacagc acctccgtta taaggaatat atctcattga gataaccggt tctccagcat
     1921 ttttaagaat gctcatactt gggggagcaa catcaatcca tgctaaatca catagtggac
     1981 cgctgactcc atgccgcctg taagcattgc ttaaagaacg gtttaacatc tgaatatggg
     2041 cagatgtatg cgcgaatcca acagccagca tcactgaagg aattctatat ttagcagcaa
     2101 ccaatgggcc tgccggatca agagggggat agactattaa atctggcctc cattttcctg
     2161 caaaatcgat gaggttatct gccatttcat cgctaaaaaa agagaaatga ccaataatat
     2221 tacttttttt cctcaactct tcctgatggc gatatccagc ctctgaatct aaacctggaa
     2281 ctgcatcaaa aacaaccagt ccagcttctg ctgctttatt cgcgaatttg ccagcgctac
     2341 taattactac atcatgtcca attacacgaa aggcctgagc cagagaaatc aatgggtata
     2401 acaaaccgta cagcggtggg ccaataaaaa gaatacgttt tcgcattaaa aaagtccgtc
     2461 ttctgcttac ctgtttattt ttctatctct aagaaaacat aattaatatt atttttatgt
     2521 gagcttatgc ac
//
LOCUS       DAZKIF010000296         2473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZKIF010000296 DAZKIF010000000
VERSION     DAZKIF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.298"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            218..1165
                     /gene="stxA1a"
                     /locus_tag="ACHOTW_004942"
     CDS             218..1165
                     /gene="stxA1a"
                     /locus_tag="ACHOTW_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP9846860.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1175..1444
                     /gene="stxB1a"
                     /locus_tag="ACHOTW_004943"
     CDS             1175..1444
                     /gene="stxB1a"
                     /locus_tag="ACHOTW_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP9846861.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            1955..>2473
                     /locus_tag="ACHOTW_004944"
     CDS             1955..>2473
                     /locus_tag="ACHOTW_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9846862.1"
                     /translation="MAFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKLTGGEGAVISATSDPGYYFVVVLAGQSNGMSYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKK"
BASE COUNT          642 a          489 c          638 g          704 t
ORIGIN      
        1 catcctgtgt atcaataaat gttgttgtct acgtacgtca agtagtcgca tgagatctga
       61 ccagatatgt taaggttgca gctctctttg aatataatta tcattttcat tacgttattg
      121 ttacgtttat ccggtgcgcc gtaaaacgcc gtccttcagg gggtggagga tgtcaagaat
      181 atagttatcg tatggtgctc aaggagtatt gtgtaatatg aaaataatta tttttagagt
      241 gctaactttt ttctttgtta tcttttcagt taatgtggtt gcgaaggaat ttaccttaga
      301 cttctcgact gcaaagacgt atgtagattc gctgaatgtc attcgctctg caataggtac
      361 tccattacag actatttcat caggaggtac gtctttactg atgattgata gtggcacagg
      421 ggataatttg tttgcagttg atgtcagagg gatagatcca gaggaagggc ggtttaataa
      481 tctacggctt attgttgaac gaaataattt atatgtgaca ggatttgtta acaggacaaa
      541 taatgttttt tatcgctttg ctgatttttc acatgttacc tttccaggta caacagcggt
      601 tacattgtct ggtgacagta gctataccac gttacagcgt gttgcaggga tcagtcgtac
      661 ggggatgcag ataaatcgcc attcgttgac tacttcttat ctggatttaa tgtcgcatag
      721 tggaacctca ctgacgcagt ctgtggcaag agcgatgtta cggtttgtta ctgtgacagc
      781 tgaagcttta cgttttcggc aaatacagag gggatttcgt acaacactgg atgatctcag
      841 tgggcgttct tatgtaatga ctgctgaaga tgttgatctt acattgaact ggggaaggtt
      901 gagtagtgtc ctgcctgatt atcatggaca agactctgtt cgtgtaggaa gaatttcttt
      961 tggaagcatt aatgcaattc tgggaagcgt ggcattaata ctgaattgtc atcatcatgc
     1021 atcgcgagtt gccagaatgg catctgatga gtttccttct atgtgtccgg cagatggaag
     1081 agtccgtggg attacgcaca ataaaatatt gtgggattca tccactctgg gggcaattct
     1141 gatgcgcaga actattagca gttgaggggg taaaatgaaa aaaacattat taatagctgc
     1201 atcgctttca tttttttcag caagtgcgct ggcgacgcct gattgtgtaa ctggaaaggt
     1261 ggagtataca aaatataatg atgacgatac ctttacagtt aaagtgggtg ataaagaatt
     1321 atttaccaac agatggaatc ttcagtctct tcttctcagt gcgcaaatta cggggatgac
     1381 tgtaaccatt aaaactaatg cctgtcataa tggaggggga ttcagcgaag ttatttttcg
     1441 ttgacttaga atagctcagt gaaaatagca ggcggagatt cataaatgtt aaatacatct
     1501 caattcagtc agttgttgcc ggtctgataa tagatgtgtt agaaaatttc tgcatggtga
     1561 atccccctgt gcggaggggc gactggtgaa cggtatgatc tctttgatga tcgtaagcga
     1621 gaatacgcgg gtttggtggc accaggccga actcaccggg aggcacccgg caccatgcaa
     1681 tggcacatag cgccactctc cagcccctct ccggaggggc tttcttatgg acaaaaaaag
     1741 cccgcgcagg gagacgctgg cggcaaggaa taaacaacaa aacgtgaagt aatatttcag
     1801 ctggcgaata atatccgaca gtaatcactc tgcgcaatag cgcggccttt ttcgtattgc
     1861 gggctgttgt ctctcttctg ccattgtcct gtaacttccg gatttcagcc cgctcatcat
     1921 tttactcaca atattatccc ggccgggagg attcatggca tttaaacact acgatgtggt
     1981 cagggcggca tcgccgtcag accttgctga tgcacttgcg caaaaaattc gtgaaggatg
     2041 gcaaccatac ggtgggccgt tttcttcgta tacggatgat ggcgcagcac ttattcaggc
     2101 gattgtcgca gaaggtgatg tgagcacacc tgttgtggtg aagctgacag gtggagaagg
     2161 tgcagtaatc agcgccacca gcgaccccgg gtattacttt gttgtggttc tggcagggca
     2221 gtcaaacggc atgtcgtatg gtgaaggtct tccgctgccg gagacatatg accgtccgga
     2281 cccgcgcatt aagcagctgg cgcgtcgcag tacggtgaca ccgggcggtg tcgcctgtaa
     2341 atataacgac atcattccgg cggaccattg tctgcatgat gtgcaggaca tgagccgcct
     2401 taaccatccg aaagcggacc tgtcaaaggg gcagtacgga accgtggggc aggggctgca
     2461 tatcgccaaa aaa
//
LOCUS       DAZKIF010000297         2460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZKIF010000297 DAZKIF010000000
VERSION     DAZKIF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.299"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..389)
                     /locus_tag="ACHOTW_004945"
     CDS             complement(<1..389)
                     /locus_tag="ACHOTW_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFP9846863.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKLKFDSLNPVNGLKRIFGLKT"
     gene            complement(398..1165)
                     /gene="sctT"
                     /locus_tag="ACHOTW_004946"
                     /pseudo
     CDS             complement(398..1165)
                     /gene="sctT"
                     /locus_tag="ACHOTW_004946"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(1167..1427)
                     /locus_tag="ACHOTW_004947"
     CDS             complement(1167..1427)
                     /locus_tag="ACHOTW_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9846864.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1438..2103)
                     /locus_tag="ACHOTW_004948"
     CDS             complement(1438..2103)
                     /locus_tag="ACHOTW_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9846865.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(2093..>2460)
                     /gene="sctQ"
                     /locus_tag="ACHOTW_004949"
     CDS             complement(2093..>2460)
                     /gene="sctQ"
                     /locus_tag="ACHOTW_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system cytoplasmic ring
                     protein SctQ"
                     /protein_id="HFP9846866.1"
                     /translation="LIYPEELKMADHFQYEEDFETDDFDIKKSESEIYDENDEQMINS
                     FEELPVKIEFVLGKKIMNLYEIDELCAKRIISLLPESEKNIEIRVNGALTGYGELVEV
                     DDKLGVEIHSWLSGHNNVK"
BASE COUNT          843 a          513 c          375 g          729 t
ORIGIN      
        1 gtttttaagc caaatatacg ctttaaacca tttactggat tcaatgaatc aaactttaat
       61 tttacagctt cagttgctaa ttgcatttta ctttgaaacc aggagacaag tattgttgtg
      121 aacatgcaaa ccagcaaaaa aggaatgatc gttttcaacc aaccaatccc catagcttta
      181 aaatagtccc aaatatccgg tttagcgtta tgatcaagga tatattcaag aatcgacata
      241 atgtgatgca catcaaaaac atatccgaga tataaagtac ccacaagcat tattacagaa
      301 acggttaagt ctctactttt tagaatttgc ccttttttag aagcatcctg cagtttcttt
      361 tgtgttggct tttcagtttt atttgccatg aaacactcta tttctcaatg tatagttgca
      421 attgttctgg gaaaaaggat aacggcatca ctcgttcagc cagtattggg ccaaaataaa
      481 taattaatat taaaaatgcc agcgcacttt tgacagtcag tgaaatggca aaagcattta
      541 gttgtgaagc atagcgtgcc agcaatccca ggacagcctc accacccagc ataacggcaa
      601 ttacggggct tgcgtaaacg atagtatgag tcattatatt attaatatat gaaaataaag
      661 gctctaattt aggaaaatta aaacttcctg aaggccataa attataactt tgctaaagag
      721 tttccagaat aaagttcaat ccaccgttag taagataaac cgctgcagag aaaaggttaa
      781 ataatcttgc taattccgag gtatcgacac ctgtagccgg atccaaagta ctacttaacg
      841 ttgcaccgcg ttggttatcg ataaagctac ctatagcaag gaaaatccaa aatggagatg
      901 ctaataagca agcaatgaac agccctatga taatttctct tgcagctaac aaagcgatat
      961 cgatttcatt taaggtcgat aattcatagt ggtaatgctg aactaatgca catgatacca
     1021 ctattattat tggatgacgg actactgttg gtatattccc actggcgaaa aatggtaaca
     1081 aatagaaagt tggtgccaat cggcaaaaac cgagcgctgt agcagccagt agactatgca
     1141 actgatagag tatcacctcc cccatgctat cctctcgcaa atgctaaatt cagcatttca
     1201 ataccaaaag aatataattt ttcaccatac catccgctca ttaaaaaaaa gcaaatactt
     1261 acgcacaata atttcacgcc aaatggcaac gtctgttctt gcaactgcgt taccgtttga
     1321 aaaagcccta ccaataaacc aacaaatgtc gctactgcaa taggtcctgc agacataact
     1381 aaaatcagat acagcgccct attccctgca aaaacaatgt catccatatt tttttcctca
     1441 gggattaatg gataggtcaa aatattgtaa tatgagtcct tttgataaca ttgtccaacc
     1501 gtccatcgcg acaaacagaa ttaattttat cggtgtagat atggttaccg gactcatcat
     1561 catcatacct aacgttaaca gaacgctgga tataaccaga tccactacaa caaagggtaa
     1621 atagatataa aaacctataa taaatgcact ttttatctca cttaacgcat atgccgggag
     1681 taacgagaaa attgaaatat tatcatcatc gattatctct tcattgtctt cagaactatt
     1741 caccttctga atcttttcaa aaaagctaac caactccggt tcagaatatt ttatcaaata
     1801 tgatttataa ccactcatgc ctgtctcaac aaaattaaca accgacgcta cattattgaa
     1861 tgaaagattc tcattctgac tgttataata tatttccttc ccaaccggca tcattacaaa
     1921 cattgacaat agcaaagcaa ccccattaag tgtcatattt gatggcactt gctgaagtcc
     1981 caatgcgttt cggacaataa caaaaacaat cgagaattta ataaaacatg tcccggatgc
     2041 tattataaac ggcaatagtg taaataatga tagtatcgct atcagcgaaa tgctatttga
     2101 cattattatg ccctgataac caagaatgaa tctctacgcc caatttatca tccacttcaa
     2161 caagttcgcc atagccagtt agcgcgccat ttacacgtat ctctatattt ttctcagatt
     2221 caggaagcag agatattatt ctttttgcac agagctcgtc tatttcatat agattcatta
     2281 ttttcttacc aagaacaaac tcaattttta caggcaactc ttcaaaacta ttaatcatct
     2341 gttcgtcatt ttcatcataa atctcactct cgcttttttt gatatcaaag tcatctgttt
     2401 caaaatcttc ctcgtactga aaatgatcag ccatttttaa ctcctctgga taaattaaat
//
LOCUS       DAZKIF010000298         2458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZKIF010000298 DAZKIF010000000
VERSION     DAZKIF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.300"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            125..328
                     /locus_tag="ACHOTW_004950"
     CDS             125..328
                     /locus_tag="ACHOTW_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP9846867.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1375
                     /locus_tag="ACHOTW_004951"
                     /pseudo
     CDS             328..1375
                     /locus_tag="ACHOTW_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1403..1492)
                     /locus_tag="ACHOTW_004952"
     tRNA            complement(1403..1492)
                     /locus_tag="ACHOTW_004952"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Ser,seq:cga)
     gene            1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHOTW_004953"
     CDS             1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHOTW_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP9846868.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
BASE COUNT          728 a          519 c          577 g          634 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacct
     1321 gaaaatattc caagaaaata ttccaagtaa ttccaagtgt gatttttgtc actgacttaa
     1381 tgatgtgtaa gtgattgaat tttggcggag agagggggat ttgaaccccc ggtggagttg
     1441 cccccactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
     1501 ggttgccatg atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc
     1561 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     1621 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac
     1681 ggaacaggaa caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct
     1741 tgttccttta cagggctttg aactggattc attaagaagc tgccggatag cacttctatt
     1801 ttgcctaccc gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat
     1861 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     1921 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     1981 ggatatacaa gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca
     2041 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga
     2101 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     2161 attggttggc gagaatgcgg cgagcattga tgcttatgct gccagtgatc ctgctgaatg
     2221 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     2281 ttcattgtgg caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca
     2341 cgctaatgat acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat
     2401 taattaacca attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaa
//
LOCUS       DAZKIF010000299         2449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZKIF010000299 DAZKIF010000000
VERSION     DAZKIF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.301"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..514)
                     /locus_tag="ACHOTW_004954"
     CDS             complement(<1..514)
                     /locus_tag="ACHOTW_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFP9846869.1"
                     /translation="MNLALRKIIYAPISYIHPQRVSLNNTPINNPVLRSITNEMILLQ
                     YNLSVEHFNLNSSLIYYINNWNLLPLICLLSGCHFYRERFAERGFFYKVPDVLRDYLS
                     AIPLEINEKARYKPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHILS
                     PLPLASTLLER"
     gene            complement(530..1264)
                     /locus_tag="ACHOTW_004955"
     CDS             complement(530..1264)
                     /locus_tag="ACHOTW_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFP9846870.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            complement(1261..1593)
                     /gene="sctI"
                     /locus_tag="ACHOTW_004956"
     CDS             complement(1261..1593)
                     /gene="sctI"
                     /locus_tag="ACHOTW_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFP9846871.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALTSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            complement(1613..1852)
                     /locus_tag="ACHOTW_004957"
     CDS             complement(1613..1852)
                     /locus_tag="ACHOTW_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFP9846872.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            complement(1866..>2449)
                     /locus_tag="ACHOTW_004958"
     CDS             complement(1866..>2449)
                     /locus_tag="ACHOTW_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP9846873.1"
                     /translation="TNSLDEAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFP
                     DLQYFKLQLLDASNPRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVL
                     ESQAIETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRF
                     NVNLKNQDTNNSSFSYGDNRFEKSQGSKWTFQE"
BASE COUNT          739 a          465 c          421 g          824 t
ORIGIN      
        1 ttctctctaa aagtgttgat gcaagtggta acggcgatag tatgtggtca acaaaatcag
       61 gaaataataa attaaatcgt tgctgcattg ccaatggttg ttgccttata tagggtaaca
      121 acgttgaaaa accacatgtg atgatattgt gatagttagc gatgccgggt ttgtatcgtg
      181 ccttttcatt tatttccagc ggtatagctg ataaataatc ccttagtaca tcaggaacct
      241 tataaaaaaa cccgcgttct gcaaaccttt ctcgataaaa gtgacaacca ctgagtaaac
      301 agatcaatgg aagtaaattc caattgttta tgtagtaaat aagtgaacta ttcagattaa
      361 aatgttcaac tgaaagatta tactgcagga gaatcatttc attggtaata gatctcagca
      421 ccggattgtt aataggtgta ttattcaagc ttactcgttg aggatgaata taactaattg
      481 gagcatatat aatctttctt agggcaagat tcataacgtt ctcatatgtc tacttaattt
      541 tcaacctgac taaattagtt agaatggccc tatacttcca taacagccag caagtcgcta
      601 cggatattaa tgcaagtaag atagaaaccg gcatagcctt atcataagca aaaacaggtt
      661 cgctaatttc atatgttggt gcttgctcaa taatgtctct tcgttttgac aatacaacag
      721 aaatattctc atattgtacg cttgcaaaac tattaacaat aaatctcttt atgtcattta
      781 tttttatttc tggattgata tctttttcat atactgcaag tacagaaata tgaattggta
      841 aagcagtttt accactatcg ccagtatcaa catcgtaact aacatgtact ctcgaagaaa
      901 caataccatc cataattttg agagattgct ctaaccgctg ctcaatagca gaatatagcc
      961 ttgctttttc cgctcgtgga gatgaaacga gtgcatctgc agggaacatc tgcgatattt
     1021 gaatatcagg cttacccggg agattgtaga tttttagcca atccaccgca gaagcaaaat
     1081 ccgttggttc aacatagatt gagaatccag ttttgccttg atccttcttt tcagcattaa
     1141 tattatgtct ttgtaaaaca gcaaggacat cattagcttg ctgttgatca agatggttca
     1201 gtaattcctg ctgcttgcag ccgcacaaca gcaggataaa caataataat gatatatatt
     1261 tcatgagcgg gtcaacgtct caactgcagc gacaattttt cttgctaatg ttcccaccaa
     1321 tgaaacctcg acattataat taagcacatg atcctgcaag actaataatt tttcaggagt
     1381 taatcctttt ttatcagacg ttaatgcttg taacgcattt tgctgccaat tttcagtttg
     1441 tacagcatag ttagcaaaag cggagatcat tttatcatcc gggttgtcaa gattgagaac
     1501 ccggtcattt aaatcaatca ctttcgcttg ctgtatttca tgagcagttg attgctcagc
     1561 tctatcaata ggtggcatat tgcttactga cataataaaa tccttatcca gtttagcgga
     1621 agttttgtat gattgccgaa tcaagatctt tatacgcttt tacaacgtta gattgcgcat
     1681 ttcgatataa tgtatactca gctaatgcac tttgatattc ggcaaggaat ttcgggtcgg
     1741 aaggattggt tgctaaatcc tccaacgctt tttcaacttg ctggttcaga tcatcaacgc
     1801 cctgatcaaa ttgtttgctg atgtccataa tataaccatt ccaatctgcc ataagtcctc
     1861 ctcaattatt cctgaaatgt ccacttgcta ccttgagact tctcgaatcg gttatctccg
     1921 tagctaaatg aactattatt tgtgtcctga tttttcaaat tcacattaaa tcttacatat
     1981 tgtttacccc attgtttata atattcgtca acatagttat ttattttgtt taattcactg
     2041 tcgcttaacg taccaatgat attgaaatac acattgttag ctgttttata tttttcataa
     2101 gaaagaccta ttgcagacaa tgtttcaata gcctgacttt ccaatacatt atcatctaat
     2161 accgcaatac ttatattgct ggcatatggc atagtttgta gtaacccttt aataagattg
     2221 tcgattagtt gctgtgtgat agcattccgt tgcttactca cggtcagtct gggattactg
     2281 gcatctaata gctgaagttt aaaatattgt aaatcaggga aattatcagc aagccatgaa
     2341 aaaattcgta tcgattcatc atcaggattt atcacccgta ctggctttcc tggttgattt
     2401 ttttccagtg cttgtttgac ccagactgct tcatccaaac tattggtat
//
LOCUS       DAZKIF010000300         2417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZKIF010000300 DAZKIF010000000
VERSION     DAZKIF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.302"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>2417
                     /gene="toxB"
                     /locus_tag="ACHOTW_004959"
     CDS             <1..>2417
                     /gene="toxB"
                     /locus_tag="ACHOTW_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFP9846874.1"
                     /translation="EEQTQKIKDIVDFLGIQDNTIKVKLESDIKPISEIQQPLHSILS
                     RQKEHVKNLLSGLLDEFSNKLRKQGLSLKTNVLSVNNFKESKINSDTVEVTVTDLQGR
                     LYRVDIDTRVIGLTFKEGINSLSEALEHMNIDAIMSVIGLVQYARMIKMNDNISAIDH
                     AGAVSDIKNIVDKFLGGILTLTNNRVYNPGGVSGASLEGFISSGLEVCASRMGGTAGR
                     YLSNVAKVIKLPLLDIGINIWSLYDSSLNHAKATTQIEYISTAIDVSFSSINTALSIG
                     AIAYPPLAIAIVPITIFSHEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSINKA
                     TGIIDLSNNQVLGNIYLDMRENPPILHGEKSYNSGKNIGSHPDMTDREIMESRAYNFA
                     CTKLSDAGEPDIFGWGDKEICNSMELSESQLANGYSNRQWPSQIPVIPEGIYNTVYLG
                     YGETLRANTEVTLSSTGYFYEIARAYTDDELSEPLLTVCNQHSHVIGGKEPLTIIIPA
                     IEHGMLGSNLHMIERFKNYNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIISFEQLS
                     RDFNLDLDLSDGRKQNFNFHHPSGFYSGKVMSITQKGINAVVGTKLGYDKIRGNNLDN
                     TFSLGSGGGVIYSGGGSNTYFVPATLQDNLHIYISEKSNGNHIILGDMHSRLSIECHF
                     SNNEREFIKMGYYNGCDVILESDTIQKIKSFAKNITIQTADGVMANWDDKLNTLSVYS
                     IDMIAWRDKNKTAKEPLPVDVIQLTNWRMHNTCSLFYDKYQVDIEANKLTYTVLFPDT
                     ELPVQLN"
BASE COUNT          843 a          355 c          476 g          743 t
ORIGIN      
        1 cagaggagca aactcagaag ataaaagata ttgtggattt tttaggtatt caggataaca
       61 caataaaagt taagcttgaa agtgatatta aacctataag tgaaatccaa cagccccttc
      121 attccattct ttcacggcag aaagaacacg taaagaattt actatctgga ttgcttgatg
      181 agttttcaaa taagttaaga aagcaaggtc tttcacttaa aacaaatgtt ctttcagtta
      241 ataacttcaa agaatcaaag ataaatagtg atacagtgga ggttacagta acagatctgc
      301 aaggaagact ttatcgtgtt gatatagata caagagttat tggcttaact tttaaagagg
      361 ggataaatag tttatccgaa gcactcgaac acatgaatat cgatgcgatt atgtctgtta
      421 ttggcttggt tcaatatgca cgaatgataa aaatgaatga caatattagt gcaatagatc
      481 atgcaggagc agtatcagat ataaaaaata ttgtagacaa atttttaggc ggaattttga
      541 ctcttactaa taatcgcgta tataatcctg gcggtgtatc tggtgcctcg cttgaaggat
      601 ttatatcttc tgggttggag gtgtgtgctt caagaatggg tggcaccgca gggcgttatc
      661 ttagcaatgt ggcaaaagta attaaattac ctttgctaga tataggcata aatatttggt
      721 cattatatga ttcttcatta aatcatgcta aagccaccac acaaattgaa tatatatcaa
      781 cagcaataga tgtatctttt tcatctataa atacagcttt gtcaataggc gcaatcgcat
      841 atcctccttt ggcaatagct atagttccga taactatttt ttctcatgaa gtaaaaaact
      901 atgctgttta tgtgaatcaa attaatgagc gccataagtt atggctcgaa gctgaaaagt
      961 atctggacaa tggaagtgca aaggttctaa gcattaacaa agccacaggc ataatcgatt
     1021 tatcaaataa tcaggtgtta ggtaatattt atttagatat gagagaaaat cctccaatat
     1081 tacacggtga gaaatcatat aatagcggaa agaatattgg tagtcatcct gatatgactg
     1141 acagagagat tatggagtct agagcataca attttgcatg cacaaaactc tcagatgcag
     1201 gtgaacccga tattttcggt tggggagata aggaaatatg taattcaatg gaattatcag
     1261 aatcacaatt agctaatggg tattctaacc gccaatggcc ttctcagatt ccagttattc
     1321 cagaaggaat ttataataca gtatatcttg gatatggaga aacattgcga gctaatactg
     1381 aagttacatt gagtagcact ggttactttt acgagattgc aagggcatat actgatgatg
     1441 aactatctga gccattatta acagtatgta accaacatag tcatgttatt ggtggaaaag
     1501 aaccattaac aattattatt cccgctattg aacatggtat gcttggctct aatctacata
     1561 tgattgaacg ttttaaaaac tataatttct cgatatcagg agggaaggga gggataaaat
     1621 taatggttgg tggtattggt gattacaaca tagaatgtac ccccggtgtt agaaatatta
     1681 tatcgtttga acaattatca cgtgacttca atctggattt ggatttatca gatgggagga
     1741 aacaaaattt taattttcat catccctcag gtttttattc aggaaaagtt atgagcataa
     1801 cgcaaaaggg tataaatgcg gttgttggta ctaaactggg atatgataag attcgtggta
     1861 ataatcttga taataccttt tctcttggga gtggaggagg tgttatatat tctggaggag
     1921 ggagcaatac ctatttcgtt cccgccacac tacaggataa tttacacata tatatatcag
     1981 aaaaatcaaa tggcaatcat attatattag gtgatatgca ctctcgccta tctattgaat
     2041 gccactttag taataatgaa agagagttta taaagatggg ctactataat ggatgtgatg
     2101 tgattttaga aagtgacact atacaaaaaa taaaatcatt tgcaaaaaat ataacaatac
     2161 agacagcgga tggagttatg gcaaattggg atgataaact aaatactctg tctgtttatt
     2221 caatagatat gattgcatgg agggataaaa ataaaactgc aaaagaaccg ttaccagttg
     2281 atgtaatcca gttaactaat tggaggatgc ataacacatg ctctttattt tatgataaat
     2341 atcaagttga tattgaagcc aataagttaa cgtatacagt cttatttcct gatacggagt
     2401 tacccgttca gctcaat
//
LOCUS       DAZKIF010000301         2415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZKIF010000301 DAZKIF010000000
VERSION     DAZKIF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.303"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..395)
                     /locus_tag="ACHOTW_004960"
     CDS             complement(<1..395)
                     /locus_tag="ACHOTW_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9846875.1"
                     /translation="MRKLYAAILSAAICLTVSGAPAWASEQQATLSAGYLHVSTNAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDRNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYL"
     gene            complement(466..>2415)
                     /locus_tag="ACHOTW_004961"
     CDS             complement(466..>2415)
                     /locus_tag="ACHOTW_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9846876.1"
                     /translation="GAATVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVETYGVWG
                     LSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTP
                     PAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTET
                     TYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPH
                     LAVYDPTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSV
                     NTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTS
                     ITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGI
                     GAGIENTPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTI
                     TSGGNPPAFSLTPDGRLTAKNADISGSVNANSGALNNVTINQNCTIKGMLEATQVRGD
                     FVKAVSKAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSG
                     NNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKG
                     NQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          515 a          709 c          594 g          597 t
ORIGIN      
        1 agataatccc cggagaaagt cgacacacgg ctgtaagcca ctcccgccat cgcatacgcg
       61 ctgaaccatt cattcacgcg cacagacggc cccgccatta cgctgaacca gcggttacgc
      121 acggaatctt catgccagcg ggtatcgctg taacgggtaa tctggcgatt cctgtctcct
      181 gcatagctga atgacgtcac cagccccagc gtgtccgtga attcataacg gtatttcacg
      241 ttaatcccgt taagattatc gctaccggga gcgttcgtcg agacatgaag ataccccgcg
      301 ctcagcgtgg cctgctgctc agacgcccat gcaggcgcac cggatacggt cagacaaatg
      361 gctgcggaca aaatggcggc ataaagttta cgcataatta cctctcgctt ttctgcaata
      421 aaaaaggcgt cattcctgac gccctttatt ggggttataa atatttcaac gaatactgat
      481 gccggaagca gcttttttgg tcacaatcac cgtacagtcg gtgatattgc ctgccccctg
      541 attgcctttc tggaaaatct taaactccag agtgacgcta ccaccaccac taggcatatc
      601 aataactgca ctgtaactac cgggaatggc ccctttagtt tctctggatg cgattaatac
      661 gccgtttttg cgaacttcaa aaccataccc cgtgtatcgc gtgcctcctg ggttatttcc
      721 gctccccgga tcgtcatacg ctataccgtt aaaaataatg ggcggaataa taatctggcg
      781 gtcaaagtta tgatcatcgc tgatggtgac tgtaaccgta ccgtttggtg tttccgtgtt
      841 accccacgta ccgacttttt tcgggaaggc ttttgataca gctttaacga aatcccctct
      901 gacctgggtc gcctccagca tgcccttaat cgtacagttc tggttaatcg tgacattgtt
      961 gagcgctcct gagttcgcat tcacactgcc actgatatcc gcatttttcg ccgtcagtcg
     1021 cccgtctggt gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt
     1081 cagatatttc aggaacactt cattcatgaa tatctgatcg ccctgaccaa caaacatcgg
     1141 ctttgtgttg ccattcgcag gattaatcat cgcaatcctg tctgctgcca gcagcacctg
     1201 actctgcatg ccgtcggggg tgttctcaat accggcaccg atacccgcaa tataaaggcg
     1261 tccgtcctgc atctgctgca gcttcactgc ccacatgctg ttcaggttat tatttgtatc
     1321 aacctgaacc ttctgtatct gctggatcgc tgcactctgg tcttccagtt tcttattgac
     1381 ggtctgtgtt atttcattgc tgacatccgt tatggacgtc ctgatttcag tcaggtcagg
     1441 cgcaagctga ccgttatcaa tctgcgtcca cagctcctga gccagatggg ttttccctat
     1501 ctcgcctttg aaaaaatcca gatagcctga tgcatcatca ctcggctgac cgacagcctc
     1561 tacaaatgcc gatttgccaa cggtgttcac actgcggata taaaagtaat aatcatggcc
     1621 cggtttgata ttgatactgg cagctatcca gtacagcgcc gtgccaagat agcgggctgt
     1681 ggtttcaacc tgcctgatat cagcaatccg cttttccgag aaccagaact caaactgtac
     1741 cgtcggatca taaaccgcaa gatgcggcgt ggcggttatc tgaaaatagc ccggtgtcag
     1801 ctcaatccga gacggcgctg ccggtgcggc aatccggaac gataccgatg ccggatcgcc
     1861 ctgctgtccc cacgcattta ccgcccggac tgtcaacctg tagttccccg gcgccagttg
     1921 cgtgaagcgg tatgtggttt ctgtcgtcct ggccgtgctg accagccgct cactgccgtc
     1981 atccgctgcc acggtcaggc gaagcaggaa gctcacgccc ttcaccacct tcggcgtgtc
     2041 ccagcgcgcc agcacctgat attccccgct gtctgcggtg acttcggcgg tcaggtgctg
     2101 caccgctggc ggcgtgacac cattcaccgt gccgctctgg tcgccgtcaa agtgcgcccc
     2161 gttatccacg atggcttctt tttccggcac gtgctgcacc gccgtgatgg caaaggtgcc
     2221 gtccgtgttt tcccggatgg agacacagcg gaacaggcga cgacgcagtg acggcaggga
     2281 gagtccccat acaccgtatg tctccacacc atcaggcagg gtgctgacct gtatccggtc
     2341 cggcgcgggg tgtgcagtga tggccacgct caccggctta ccgctgccgt taatcaggtt
     2401 caccgtggcg gcacc
//
LOCUS       DAZKIF010000302         2404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZKIF010000302 DAZKIF010000000
VERSION     DAZKIF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.304"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            297..458
                     /locus_tag="ACHOTW_004962"
                     /pseudo
     CDS             297..458
                     /locus_tag="ACHOTW_004962"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..507
                     /locus_tag="ACHOTW_004963"
     CDS             442..507
                     /locus_tag="ACHOTW_004963"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFP9846877.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            623..880
                     /locus_tag="ACHOTW_004964"
     CDS             623..880
                     /locus_tag="ACHOTW_004964"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9846878.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ACHOTW_004965"
     CDS             complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ACHOTW_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFP9846879.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
     gene            complement(1562..>2404)
                     /gene="nleH1-2"
                     /locus_tag="ACHOTW_004966"
     CDS             complement(1562..>2404)
                     /gene="nleH1-2"
                     /locus_tag="ACHOTW_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFP9846880.1"
                     /translation="DNRVLSSVRDAAVHSDSGTQVTVGNRTYRVVVTDNKFCVTRESH
                     SGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTIERGSVHSNRPDLPPVDYAQPELP
                     PADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYEDMEDTTKVLKMFTISQSHEEVTSE
                     VRCFNQYYGSGSAEKIYNDNGNVIGIRMNKINGESLLDIPSLPAQAEQAIYDMFDRLE
                     KKGILFVDTTETNVLYDRMRNEFNPIDISSYNVSDISWSEHQVMQSYHGGKLDLISVV
                     LSKI"
BASE COUNT          729 a          552 c          425 g          698 t
ORIGIN      
        1 aaaaacaact ttttatcttt ttgtttcgct caaattagta taaaaagccg aacaacaaac
       61 aataaaaaac cattaacatc aatagattat gatggcttca atctaaataa taggctatat
      121 aatactgcaa gacacaacac atgctgtcac tatgagtcaa ctatttagat gatattagtg
      181 acctgtttca gagcattagc gcaaggcgat tctttgtctt cttgcactaa ttttttatca
      241 taaaaatgtt cctagcactg ggcatcaata tcgcaggtca gaaagagctc ctggggatgc
      301 ggctggccga aaatgaaggg gcgaatttct ggttcaatgt gctgactgaa ctgaaaaacc
      361 gcggtctgaa cgatatcctc atcgcctgtg tgtatggcct gaaagaattc ccggaggccc
      421 gcatccagtt atgcatcgtg catatggtgc gcaacagcat gcgcttcgtg tcatggaagg
      481 aatacaaagc cgtcactcgc gacctgaaag cgattagcct cccacagaag aggcaggcca
      541 gcaggcactg gaagcgtttg ctgcggcctg ggactgccgc tatccgcaga taagccggtg
      601 ctagctgtca aactggacta acttggcgac gtttttcgct tatccggcag atatccgcaa
      661 agtgatctac acaacgaacg ccatcgagtc gctgaacagt gtgatccggc atgccatcag
      721 gaaacgcaag gtgttcccga cggacgagtc agtaaaaaaa gtggtgtggc tggcaatcca
      781 ggccgcgtca cagaaatgga caatgccgtt aagggactgg cgaatggcaa tgagccgctt
      841 tattatcgag tttggtgacc gtcttgacgg tcacttttaa gaaaaggcat ttacacagaa
      901 tcctaaacag gctcacaggc ctctaatcat ccacattgta aagatccttt gttgtaagta
      961 agatctggta ccctaataat atcaaaagga ttaaaatcat cactgcatcc ttccacgagg
     1021 catttcattg ctcgtagatc accatatagc tctgaagagt gatgcggaca tagcagatta
     1081 taaactttac atttcgaatc ttctttgcct tgtctacttt cctcaatttt tactttcaat
     1141 gaatgcagaa aaaaaagatc cctgatataa ctatccctgt cctctatcgt cattcccgcc
     1201 ctcagcgctc cgacaacctc atcagtattt tcatttctca cctcaaatac actatttctc
     1261 ttatccatca gcccacttac aaaagcactt agctcttgaa aaatttcctc aagctcatta
     1321 ttatttctgg cacatgaata tatagagacc ataacatcaa gtttatcttg taattcagga
     1381 gaaagaaagt gattatgatt aagctcactt actgattccg gagtcgaagg gttacctctt
     1441 cctgatacat acatccatga ataaagattt gctgaagaac cacttgttgg taacatatca
     1501 aaaccccctt aacaaataaa tccataaata ttattgcaac taatatatct ggataaaaat
     1561 attatatctt acttaatact acactaataa gatccagctt tcctccgtga taagattgca
     1621 tgacttgatg ttcactccat gaaatatcag aaacattata agatgatata tctattggat
     1681 taaattcatt tctcatacga tcatataaaa catttgtttc tgttgtatca acaaaaagaa
     1741 ttcctttttt ctccagtctg tcaaacatat cgtaaatagc ctgttcagct tgtgctggta
     1801 atgatggaat atccaaaaga gattccccat ttattttatt cattctaata ccaataacat
     1861 ttccattatc attatatatt ttctctgcac tcccggaacc ataatactga ttgaaacaac
     1921 gaacttcgct tgtcacctct tcatggcttt gagatatagt aaacatcttc aacacttttg
     1981 ttgtatcttc catatcttca tacacgacag cattaccacc tttaccaata acattacctg
     2041 gcacgggtga tttattgttg ctaaccctcg gcaactctga ttgagtataa tccgctggag
     2101 gtaactccgg ctgcgcataa tccactggag gtaaatcagg tctgttcgaa tgaacagagc
     2161 ctctttctat agtcgtgctc actggcgatg tattcagcat agcctcaatt tttctgctaa
     2221 tctctccctt aggccatccc aacctgtgca acagattagt aaaacaacca ctatgacttt
     2281 ctcttgtaac gcaaaactta ttatcagtga ccacaacacg atatgttctg ttgccaaccg
     2341 ttacttgcgt cccgctatca gagtgaacag cagcatccct tacagaggat aaaacacgat
     2401 tatc
//
LOCUS       DAZKIF010000303         2393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZKIF010000303 DAZKIF010000000
VERSION     DAZKIF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.305"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..185
                     /locus_tag="ACHOTW_004967"
     CDS             <1..185
                     /locus_tag="ACHOTW_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9846881.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="ACHOTW_004968"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="ACHOTW_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9846882.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="ACHOTW_004969"
                     /pseudo
     CDS             563..2148
                     /locus_tag="ACHOTW_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="ACHOTW_004970"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="ACHOTW_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          612 a          609 c          677 g          495 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgcc gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag gga
//
LOCUS       DAZKIF010000304         2388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZKIF010000304 DAZKIF010000000
VERSION     DAZKIF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.306"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(112..801)
                     /locus_tag="ACHOTW_004971"
     CDS             complement(112..801)
                     /locus_tag="ACHOTW_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9846883.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(816..938)
                     /locus_tag="ACHOTW_004972"
     CDS             complement(816..938)
                     /locus_tag="ACHOTW_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9846884.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            1276..2235
                     /locus_tag="ACHOTW_004973"
     CDS             1276..2235
                     /locus_tag="ACHOTW_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFP9846885.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          777 a          515 c          430 g          666 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaccc actcggcagc
       61 gggtttatac attttttaca acataccaaa tttgcatgaa gtatatggct tttaatccag
      121 ttttgcaata ttttgctgta aaaatgctgc cttttgtttt gaacgtgttc tcgtcacaag
      181 caataaagca tcactatcaa gctgtagaaa aatgtgcttc attgcaaccc agcgttcagt
      241 aaatgtctcg gaccagtttt ttgttgtcac tcccaccaat gatgccagtg tctggtattc
      301 ataggcctca cgccctgcaa gttcgctctt cacatcctgt gcagccagcc agattaacgt
      361 cttcaggcga tccagtgtct tacctgcaat ttttctgtta cttaacaaat ctttaaactc
      421 gctccatgcc cattgcgtta tggtgacctg atgcccccat cgaacgcttt cgctgtaaca
      481 ccaaagcaac catgctttct gatgttcatc gagagacaaa accgcgcggc gccatgaaga
      541 ggttgagaat tcaaccgggc tgaccaaagc aatggatgaa ccttttgcgt acgactgctt
      601 accggaagtc ggcgtattat ccagcgtaat catcttgcca gttaccacat ccagaatgcg
      661 cggcttcttt cgtttgtatg taccagtatc aaattgtgca tgctcctgcc aggcttcgag
      721 ctggcctttc gttgctccgt tcaagtcagc agtagctgcc ataagttgct cacgaacata
      781 ctgtaaatat tgggtattca tgcagtaaat cctttctata ttttggcata attcttcaac
      841 attcggtaat cgttcaaaac cgaatcgggg aaacgacata agcacaggag cccccagcga
      901 cagcgaagga gttctgatat ataagactca gacatcattc attccccggt tctccaatat
      961 ctgtttcact catcatccat aactacctgt aattgccccc cctttttttg taacagttct
     1021 tatattgcta tatagaatag ccattactaa tgcatttaaa tttaataaaa taaaattata
     1081 aaaaacataa aacaccacgc aaacacactt aataaaaaca ccgttacatt aaaagataat
     1141 aaaaaccgca ataaaaaacg aataaatcaa ttgtctcacg caattataaa acatcatatt
     1201 gattacgcac cttgcattac aaactcatgt atgtaaaata cgcgcaccat tcaaaaaaaa
     1261 ggaagacaat aacatatgaa aaaaagtgtc atcgctggcg tctttattgc tctgtcattt
     1321 accacgtgtt cagctatcgc gaacagcctt gcattatcat tagcaaatga tgatgcaggg
     1381 aagtttcaac caatacttaa tgatatttat ggcaataaac atgaaaacag agatgattac
     1441 tcacaaggct tatttctggg atatagccac gatatctcag actcgagcca attatctctc
     1501 catattgcgc aagatattta ctctccatca ggcagtaata aaagacacaa cacagctgta
     1561 actggagaca gagcttttag tgcatacact cacactggta ttgaatggaa ctcccttgcg
     1621 aatgactgga ttcgctatcg attaggtact gacataggtg ttgttggccc cgacgcaggc
     1681 ggtcagaaag tacaaaataa agctcatgag attattgggg cagaaaaata tcatgcatgg
     1741 gatgatcaaa tagagaatcg ctacggttat actgtaaaag ggatgctatc catgacacca
     1801 agtatggata ttttaggtgc taatgttgga ttataccctg aagtttctgc tgttactgga
     1861 aacttatttc aatatgtagc atatggcgca accattgcca ttggtaatga taaaaccttc
     1921 aattcggata atggctttgg tctgctggct ccccgtggtt taatgcatat gtccgataca
     1981 agcggattca aatacaagat ttttgcaggt atggaaagac gagatgtcaa tcgcaactat
     2041 actctcgaag gaaaaacaat acagacgaaa caaacaacag tatcgctaaa caaaactgtt
     2101 gatgaatatc aagttggcgc aacaattggg tatgcacctg tagccttcac actagcattt
     2161 aataaagtaa catcagaatt caagacaggg gatgactatt catttataaa tggagcaatc
     2221 accttctttt tttaactgaa ttgaattcaa tcaaaataac ataagtccaa caaaaacata
     2281 aagtgcgaaa tgaatgccag ctccatttat ttcgcactat aaaagattaa aagttgcaat
     2341 aaaataataa aatgactcag ttacgaaaac caataaactg tggccagt
//
LOCUS       DAZKIF010000305         2334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZKIF010000305 DAZKIF010000000
VERSION     DAZKIF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.307"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..473
                     /gene="eco"
                     /locus_tag="ACHOTW_004974"
     CDS             <1..473
                     /gene="eco"
                     /locus_tag="ACHOTW_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP9846886.1"
                     /translation="AVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQVIQLTPQ
                     EDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVSTMMACP
                     DGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVVR"
     gene            complement(623..2269)
                     /gene="mqo"
                     /locus_tag="ACHOTW_004975"
     CDS             complement(623..2269)
                     /gene="mqo"
                     /locus_tag="ACHOTW_004975"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP9846887.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          531 a          653 c          591 g          559 t
ORIGIN      
        1 ctgcagtatt gtttgccgct ttcgccacca cttccgcctg ggcggcagag agcgtccagc
       61 cactggaaaa aatcgcgcct tatccacaag ctgaaaaagg catgaagcgt caggtgattc
      121 agttaacccc gcaagaagat gaatctaccc tgaaagtaga actgttaatc ggtcagacgc
      181 tggaagtcga ttgcaatttg catcgtctcg gcgggaagct ggaaagcaaa acgctggaag
      241 gctggggcta tgattattat gtctttgata aagttagctc gccggtttcc acgatgatgg
      301 cctgcccgga tggcaagaaa gagaagaaat ttgtcaccgc gtatctgggc gatactggaa
      361 tgctgcgtta caacagcaag ctgccgatcg tggtgtatac gccagacaat gtagatgtga
      421 agtaccgcgt ctggaaggcg gaagagaaaa ttgacaacgc ggtagttcgc taaactgccg
      481 tgaagtgcgg caccccgtag gttggataaa gcggtcacgc cgcatccgac atctaacgcc
      541 cgagccggtt gcctgatgcg acgctggcgc gtcttatcag catacgccac atccggcata
      601 ccatgccgga tgtggcacat cattacaacg caatatccgc cacttctttt tgaacgggtt
      661 gcggtttcaa ctgcggtttc ggcgtactat ctgctgcttg cggtttgtcg tatttcagcc
      721 ccagcacttc gctggtgtac tgcaactcgc gttctgttgc cgctacatca ccgttcagct
      781 tgcgtccata agatggaacg atcgctttca acgtagcctg ccattgcggg ctggaaacgc
      841 gatcgccaaa tactttttcc agcagattca acataatcgg cgcggcggtt gacgcccctg
      901 gcgatgcccc caggagcgcg gcaatggttc cttgctggtc actgaccact tcagtaccca
      961 gacgcagtac accaccttta tcggcatcac gcttgataat ctgcacacgc tgccccgctt
     1021 gccacaaacg ccagtcctct tttttcgctt gcggatagta ctctttcaac gcttcaaaac
     1081 gatcctcttc actcaacatc acctgactca ccagatattt caccagatcg aaattatcca
     1141 gcccgacgtg catcatcggc atcacgttag aggtggtggt ggaactcatc agatcccaca
     1201 atgaaccgtt tttgaggaat ttggttgaga aggtggcaaa tggcccaaac agcactacgc
     1261 gtttaccgtc cagaacgcgg gtatcgatat gcggaaccga catcggtggt gcgccaacgg
     1321 atgctttacc gtaaaccttc gccagatggt gattaaccac gtccgggttt tccgaaacaa
     1381 ggaactgtcc gcccaccggg aaaccggcgt agtctttcgc ttccggaatc cccgattcct
     1441 gtaacagctt cagtgccgca ccgcccgcgc cgataaagac aaatttcgca cggatgttct
     1501 gtgcagtgcc atttttcaga tcggcaacgg taacggtcca ggtgttatcg tcattacgct
     1561 ttagggcgcg gacttcgctg ctgagttgca gcgagaagtt agatttcttc tgcaaggaag
     1621 caattaactg gcgggtgatc tcgccgtagt tcacatcggt accaatttcc gtacgcgtgg
     1681 ctgccacttt ctgttgcgga tcgcgccctt ccatcactaa cggtgcccac tctttgatct
     1741 gcgcgtgatc ttcagagtaa cgcataccgc gaaacagcga gctttgttgc aacgcggcgt
     1801 aacgggcgcg caggaaattg acgttatcct cgccccagac aaagctcata tgcggaacgg
     1861 tattgataaa tgaacgcgga gtacgcagca cgccgcgctc gacctggtgc gcccagaact
     1921 ggcgggaaat ctgaaatgct tcgttaatgg cgactgcttt ttcaatactg atgctgccat
     1981 cggcgttttg cggggtgtag ttcagttcca tcagtgcaga atgcccggtt ccggcgttat
     2041 tccagccgtt cgaactctcc tgcgcgacac cctccaggcg ctccaccatg gtcatcgacc
     2101 attcaggctc cagctcgcgt aaataggtcc ccaacgtggc gctcataatg ccgccgccaa
     2161 tcaacagtac atcagtttcc tgctcctcgg acgctttcgc ttttgccgcc atcgaaacgg
     2221 cattaagccc cacggccatc gagaagagca tggcagtcac ttttttcatc ttgttaatgc
     2281 cttactttta gtcgctttat tgcaggtgag atttgcgcgg catcaacggt aaca
//
LOCUS       DAZKIF010000306         2299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZKIF010000306 DAZKIF010000000
VERSION     DAZKIF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.308"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..446)
                     /gene="escC"
                     /locus_tag="ACHOTW_004976"
     CDS             complement(<1..446)
                     /gene="escC"
                     /locus_tag="ACHOTW_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFP9846888.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKSEYFIITKSSPVRA
                     ILNDFAANYSIPVFISSSVNDDFSGEIKNEKPVKVLEKLSKLYHLTWYYDENILYIYK
                     TNEISRSIITPTYLDIDSLLKYLSDTISVNKNSCNVRKITTFNSIEV"
     gene            complement(446..901)
                     /gene="sepD"
                     /locus_tag="ACHOTW_004977"
     CDS             complement(446..901)
                     /gene="sepD"
                     /locus_tag="ACHOTW_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFP9846889.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQLSSSDTMMPPTHLFSDIGRIV"
     gene            complement(907..1479)
                     /gene="escJ"
                     /locus_tag="ACHOTW_004978"
     CDS             complement(907..1479)
                     /gene="escJ"
                     /locus_tag="ACHOTW_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFP9846890.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(1482..1859)
                     /gene="sctI"
                     /locus_tag="ACHOTW_004979"
     CDS             complement(1482..1859)
                     /gene="sctI"
                     /locus_tag="ACHOTW_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFP9846891.1"
                     /translation="MNNINQSENINIQLNKAPQTNFVDEHTSLASAPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNSDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(1943..2242)
                     /gene="espZ"
                     /locus_tag="ACHOTW_004980"
                     /gene_synonym="sepZ"
     CDS             complement(1943..2242)
                     /gene="espZ"
                     /locus_tag="ACHOTW_004980"
                     /gene_synonym="sepZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE cytoprotective
                     effector EspZ"
                     /protein_id="HFP9846892.1"
                     /translation="MEAANLSPSGAVMPLATSLSGNNSVDEKTGVIKPENGTNRTVRV
                     IAGLALTTTALAALGTGIAAACSETSSTEYLALGITSGVLGTLTAVGGALAMKYA"
BASE COUNT          643 a          433 c          439 g          784 t
ORIGIN      
        1 acttcaatcg agttaaatgt cgtaattttt ctcacattgc aagagttttt gtttactgag
       61 attgtatcac tcagatattt tagcagtgag tcaatatcca gatatgttgg tgtaattata
      121 cttcttgata tttcatttgt tttataaata tacaagatgt tctcatcata ataccatgtg
      181 agatgatata gtttggacag tttttccagt acttttacag gtttttcatt ttttatttcg
      241 ccagagaaat catcattcac ggaactactg atgaaaacag gaatgctgta gttagcagca
      301 aagtcattaa ggattgcacg tactggagaa cttttagtaa taataaaata ttcgctcttg
      361 cctaatcttt tttccagaga ggacggggca gcttgtgcac tgcagcaaaa tagtgctgta
      421 aaaataaaaa aacttatttt tttcattaca caattcgtcc tatatcagaa aacaagtgtg
      481 ttggcggcat cattgtatcc gatgaagata attgctctag tttatcaaat tgcttatcga
      541 gcaaagaggc gagagtatgt tgcattattg cttttgacat gtttgcgcga tgattattat
      601 ctttatgaaa aatttggcaa agataccact tcttattaga caggaaaata cccatttgtt
      661 tatattcgtt actaacggcc aggatattgc agagtctgat aaatgtatct gttggaacct
      721 gacgaaaatc accactaagc tcaatatata gaacccagta gtttccaaaa tcgtgcaaga
      781 aagcattttt ttggtagata taaaaagtga gatgcgttgg tgaaccgttg acatccttta
      841 caaaatcaag ggccgttagc cagtcacaat cattctttgc tatgccatta ttattgttca
      901 tacatattac ccgtcctgtc ctgaggatga cttgataaca acactaatat tttctaattt
      961 gagatcgtca acactattct taactaggtt ttttatctga atgaccgatg gtgctaagtt
     1021 aacctctggc gagctgatga ctaaaacggc tgctgaggag ggttgacttt cgttattgtt
     1081 aacatttaat gaaacgctgc aatcgataac accaggtatt ttacttagca acctttcgat
     1141 gtcttgttct ttgagatagt tgatttttgc attttcttgt gatggcgatg ccactaactg
     1201 gctaggtggg aaaataacct cgatatctgc aaatttcttt ttcgggaagc cgttattatt
     1261 taaaatcgtt attgcgcgaa caaaatcctc tttttccaca ctgagtgtca tatttccact
     1321 tttgtccatt tctttggaaa cattgacatc attggatagc aacagtgctt gcatctgatt
     1381 agcttctttt tctgttaatc ctgtatacaa ttgctcttta catgccactg tcaaacaaat
     1441 agcagccaat aaaaaaaggt ttttaatgtg ttttttcatt tttattgcat cgaaactaat
     1501 tttgtaaccg atgttgaaag gaggcttgct aacttagata cgacatcaac gttgacgttt
     1561 gcgttgagca cattcttttg taaggtgagc acgtcttctg gcgagagccc acctgcagat
     1621 agagcatcga aactgagatc gctgttcatt gttgcaagat gtctcttttt aatttcttca
     1681 atcaacactt gttctggaga agacactccc gctgttttag ggagtaattg atcaagaaac
     1741 tgagccgcac ctgccgcaga gggggctgag gctaacgagg tatgctcatc aacgaaattt
     1801 gtttggggcg ctttatttaa ttgaatatta atgttttccg attgattaat gttgttcata
     1861 tttactgtta gcctcttttt atgaaagagg caagggcata atacatccat tacctctatc
     1921 ttttatttat gcgatgggcc ggttaggcat atttcatcgc taatgcaccg ccaaccgcag
     1981 taagagtacc tagtacgcca gaagtaatac ccagggctaa gtattctgtg ctgctcgtct
     2041 ccgagcatgc cgctgcaata cctgtaccta gagctgccag agccgtagtg gtaagtgcta
     2101 atccggctat aactctaacg gtgcgatttg ttccattttc tggtttaatc actcctgtct
     2161 tctcatccac tgagttattt ccactgagtg aggtcgccag cggcattact gcaccagaag
     2221 gacttaaatt tgctgcttcc attgatcttt ctccttttgt ctaatttaga catttacctg
     2281 gttataaata accaaatta
//
LOCUS       DAZKIF010000307         2240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZKIF010000307 DAZKIF010000000
VERSION     DAZKIF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.309"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1668
                     /locus_tag="ACHOTW_004981"
     CDS             <1..1668
                     /locus_tag="ACHOTW_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9846893.1"
                     /translation="ARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVV
                     KGVRFSLRLTSGSGEGSRLVTTAITADTEHRSSGLPPGEYTLTVRAINSYGQQGEPAT
                     TTFRINAPAVPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSAR
                     YLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKI
                     GKLHLAQGLWELIDNSQLADEMAEMKTSITETRNEITQTVSKTLEDQSAIIQQIQRVQ
                     KDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEDG
                     NTTPLFVAQGNQLFMNDVFLKRLFAVSITSSANPPTFSLTPEGRLTARNADISGNVNA
                     NSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYD
                     DQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVI
                     DMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGISIS"
     gene            1736..>2240
                     /locus_tag="ACHOTW_004982"
     CDS             1736..>2240
                     /locus_tag="ACHOTW_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9846894.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNP"
BASE COUNT          533 a          556 c          669 g          482 t
ORIGIN      
        1 gcccgctttg agccgcagtc aggcaccctg aacagcgtca tccctccggc agtgcagcac
       61 ctgacggtgg aggtgagcgc agctgacggc cagtatctgg cgcaggctaa atgggacacg
      121 ccgcgggtgg tgaagggcgt gcgcttcagt ctgcgcctga ccagcggaag cggagaaggc
      181 agccgtctgg tgaccaccgc catcactgcg gatacagagc atcgttccag tggtctgccg
      241 ccgggggaat acaccctgac ggtcagggcg attaacagtt atggccagca gggggaaccg
      301 gccaccacca cgttcaggat taatgcacct gcggtacccg ccacgattga gctgacaccg
      361 ggctattttc agataacagc ggtcccgcgt cttgcggtgt atgacccgac ggtacagttt
      421 gagttctggt tttcggagac aaaaatcgca gatacatctc aggtggaaac ctctgcccgt
      481 tatctgggga ccggcagtca gtggagtgta tccggcccgc acattaagcc cgggaaggat
      541 ttctggtttt acgtgcgcag cgtcaacctg gtggggaaat ctgcttttgt ggaagccagt
      601 ggccgggcca gcaatgatgc agaagggtat ctggggctgt ttcgggaaaa aataggaaaa
      661 ctgcatctgg ctcaggggct gtgggagctg atagacaaca gccagcttgc ggatgagatg
      721 gcggagatga agacctccat caccgaaacc cgcaatgaaa tcacacagac ggtcagtaaa
      781 acgctggagg accagagcgc catcatacag cagatacagc gcgtgcagaa ggacacaaat
      841 gatgaccttg ctgcacttta catgctgaag gtacagaaaa caaaaaatgg cataccctat
      901 gttgccggta ttggagcggg gattgaggat actgatggcc agcccctgag caacatactg
      961 ctgctggctg accgtattgc gatgattaac ccggaggacg gcaacaccac gccgttattt
     1021 gtggcgcagg ggaatcagtt gttcatgaac gatgtgttcc tgaaacgact gtttgcggtg
     1081 agtatcacgt catccgccaa tcccccgacg ttttccctga cgccggaggg caggctgacc
     1141 gcaagaaatg ctgatatcag cggtaacgtg aatgcgaatt ccgggacgct caacaacgtc
     1201 acgattaacg agaactgtcg ggttctggga aaactgtccg cgaaccagat tgaaggcgat
     1261 ctcgttaaaa cagtgggcaa agctttcccc cgggattccc gtgcaccgga gcggtggcca
     1321 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgaccggca gattgttatt
     1381 ccggcggtgg cattcagcgg cgctaaacat gagagagagc atactgatat ttactcctca
     1441 tgccgtctga tagtgcggaa aaacggtgct gaaatttata accgtaccgc gctggataat
     1501 acgctgattt acagtggcgt tattgatatg cctgccggtc acggtcacat gacgctggag
     1561 ttttcggtgt cagcatggct ggtgaataac tggtatccca cagcaagtat cagcgatttg
     1621 ctggttgtgg tgatgaagaa agccaccgca ggcatcagta tcagctgaat tttataaccc
     1681 atatacgggc gccagaaatg gcgccttttt tattgcagaa aagcgagagg taattatgcg
     1741 taaactttat gccgccattt tgtccgcagc catttgtctg gccgtatccg gtgcgcctgc
     1801 atgggcgtct gaacatcagt caacgctgag cgcggggtat cttcatgtct cgacgaacgt
     1861 tcctggcagc gatgaactga acgggattaa cgtgaaatac cgttatgagt ttacggacac
     1921 actggggatg gtgacgtcgt tcagctatgc aggagacaag aatcgccagc tgacccgtta
     1981 cagcgatacc cgctggcatg aagattccgt tcgtaaccgc tggttcagcg taatggcggg
     2041 gccgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggtg tggcttacag
     2101 ccgtgtgtcg actttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
     2161 tgatgtgctg accggaagtg atgacggtcg ccacagcaac acgtctctgg cgtggggagc
     2221 tggcgtgcag tttaacccga
//
LOCUS       DAZKIF010000308         2211 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZKIF010000308 DAZKIF010000000
VERSION     DAZKIF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.310"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..683)
                     /locus_tag="ACHOTW_004983"
     CDS             complement(<1..683)
                     /locus_tag="ACHOTW_004983"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFP9846895.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTL"
     gene            complement(747..>2211)
                     /locus_tag="ACHOTW_004984"
     CDS             complement(747..>2211)
                     /locus_tag="ACHOTW_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9846896.1"
                     /translation="ALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIMMTALLLNW
                     RQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRVTDTTLKVV
                     AADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVYTTTQSNEP
                     PAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNLGYS
                     VDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLD
                     DILQRADVVTVGIDGGGLDDLLGMYVTGRDRETREWLGWGHAWAHETAVVRRKSEASR
                     FQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQILDSLAEAGI
                     PDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPKGNAILITK
                     QASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT          468 a          713 c          562 g          468 t
ORIGIN      
        1 tccagcgtgc gcccttcctc tgcggctttg gtcatgacct cctcaaggga ggctgccagc
       61 gctgcacgct tgttttcaaa acttttaatc tgttcgccaa tattcattat ggtcttttcc
      121 ttatgaaaaa cggttgttga ctgtgccgca gcgccggcag aagatgcgat tttcaccacc
      181 ggtttccggt tgccggacgc ggcagaaaac gggcggtcgt aagatttaat ggtccggatg
      241 gtgcattccg cattcgcggg cacggtgacg gcagacacct ccatcagttc ccagcgcaga
      301 aaatgcagtc cgcctccgtc cagaaaggtg tattcatggg gacggaagcc cacggacagc
      361 cccctgacca gcccggtctt aatggccgcc cagacctcat ccagccgggc agccagttgc
      421 gacggcatat ccggtacggg cttcaccagt gttgccgtga tttccagccc ttcgctgacc
      481 cggcgcaccg tacactgccc caccgggcgg gaatggtcat gctgccagag aaacgggatc
      541 gcactgccaa actccgcgcc ctccggctcc aggatgtcac catcccgatc cggagaaggc
      601 gttgacgcaa tcccggtgat cacccgttca tcctcactga aggatttcac cgtcagcagg
      661 gaacaggccc gtttaagagt cacatcagcc tcctgaaaat aaaaaaaccg ccgcagcggt
      721 tcatgatggt tacagtgtga acagggttat atgaaaaaaa ccgcatattc tttctttttc
      781 ggttccgggt taagggacat cagggagacc gcattgaaca gcgccatcag cgggtcaatt
      841 tttccccgtc cactggcctg tttggtgata agaatggcgt tacctttagg ctccacccgg
      901 gcattgccga cacaccaggc catcaggggc tggtcaccat gcaccagcac cccttcagcc
      961 agtttgcgct cggtggtttt aatggccccg cccagtttcc agccctggct tatccccacc
     1021 acaattccgt cggggatccc ggcttccgcc agtgaatcca gaatctgccc cacccctgac
     1081 gggtcaatac cgatatggtc cagtaactca gcctcatgaa tgcgacgcac atattccgcc
     1141 acttccgccg tgtcatcccc gacacgccgg acaatggtca tatctccaca ggcaacaaga
     1201 tcctgaaacc gggacgcctc gctcttccgt cggaccaccg cggtttcatg cgcccaggca
     1261 tggccccagc ccagccattc gcgggtttcc ctgtcacggc cagtcacgta cattcccagc
     1321 agatcatcca ggcccccgcc gtcaatcccc accgtcacca catcagcgcg ctgcaggata
     1381 tcgtccaggc tgacgcgcct gccctgctgc tcccagaaat ccgcacccgc ccagcggtca
     1441 gaacgcaggg caagaccaat ttccacattg gcatgttttg acatgaagcc acgaaatgct
     1501 tcctcaccag cctcccgggc tttacggtac tcccggtaca gaaaagcctc atccaccgaa
     1561 taaccgagat tcgggttaac catggcgagg ttttccatca gcaggtgagc cccgctttcc
     1621 accatttcag gaggatgctc aaaaatcacc ggcagaaagt gcggatcatg aattttgccg
     1681 tcacggacat cccgggcgta ctgcagtttc tgtctgaaca ccccggctgg cggctcattc
     1741 gactgggtgg tcgtatacac cacaaaccct tccgggcggg aggcaaggcc gcctatggct
     1801 tcacgtaaca tgtcctccgc tttgtactgc ttgccaaata accacagttc atcaatcagc
     1861 gtccccacgg acttgatacc ggataccgta ttcggatcgg cagccaccac cttcagggtg
     1921 gtgtccgtca ccctgtgggt gatggtccgg atatgtgtct gcacctgaca gaggtcatcc
     1981 agatcatcgt cccgtcgtac catatccctg gcagggttga aggcgttagc cgccacctcc
     2041 acggtcgggg ccagaatggt gtagcccgcc gcctgccgcc agttcagtaa cagcgccgtc
     2101 atcatgatcc cggcagccag cgtggacttg ctgtttttct tggggataag gataaaaact
     2161 tccttgatat ggcgtacacc ggtctgcgca tcgtaggagc caaacagggc c
//
LOCUS       DAZKIF010000309         2199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZKIF010000309 DAZKIF010000000
VERSION     DAZKIF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.311"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..423
                     /locus_tag="ACHOTW_004985"
     CDS             <1..423
                     /locus_tag="ACHOTW_004985"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP9846897.1"
                     /translation="AASDKNALCAHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVE
                     KAAEQCIQQRQTVVMLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGN
                     SKGSMLLIWRPFISPRRMFTTVSKAALMAIGLGVRRAA"
     gene            420..596
                     /locus_tag="ACHOTW_004986"
     CDS             420..596
                     /locus_tag="ACHOTW_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9846898.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            599..1000
                     /locus_tag="ACHOTW_004987"
     CDS             599..1000
                     /locus_tag="ACHOTW_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846899.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANMTYMKAYQKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            960..1169
                     /locus_tag="ACHOTW_004988"
     CDS             960..1169
                     /locus_tag="ACHOTW_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP9846900.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDG"
     gene            1162..1767
                     /locus_tag="ACHOTW_004989"
     CDS             1162..1767
                     /locus_tag="ACHOTW_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9846901.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALKLQSKQRK
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLMSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTIEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            1764..1958
                     /locus_tag="ACHOTW_004990"
     CDS             1764..1958
                     /locus_tag="ACHOTW_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP9846902.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            1951..>2199
                     /locus_tag="ACHOTW_004991"
     CDS             1951..>2199
                     /locus_tag="ACHOTW_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9846903.1"
                     /translation="MRDIRQVLECWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGGTFMA"
BASE COUNT          692 a          494 c          575 g          438 t
ORIGIN      
        1 gctgcgagcg acaaaaacgc tctgtgtgct cactggctaa ctgaggctga cgacgcgctc
       61 aattctgagt ggataagcca cggtgcaatc tggaataacc caccgtacag caatatcagg
      121 ccgtgggtgg aaaaagccgc tgagcagtgc atacaacagc gacagacggt agtgatgctt
      181 gtgccagagg atatgtctgt cggatggttc agcaaggctc tggagagtgt tgacgaagtt
      241 cgcatcatca ctgatggacg gattaatttt atcgaaccat cgacagggct ggagaagaag
      301 ggaaacagca aaggctcaat gttgctgatt tggcgaccgt tcatcagtcc tcgacggatg
      361 tttactaccg tatccaaagc ggcattgatg gcgatcgggc tgggcgtcag gagggctgca
      421 tgaggcgaca gcgacgaagt atcaccgaca tcatctgcga aaactgcaaa taccttccaa
      481 cgaaacgctc cagaaataaa cgcaagccaa tcccaaaaga atctgacgta aaaaccttca
      541 attacacggc tcacctgtgg gatatccggt ggctaagaca tcgtgcgagg aaatgacaat
      601 gcttttaatt caacctggat ttggacttag catcaaaaaa ggccacatgt ttggcgagaa
      661 agagtcacaa cgaaaaatgg tgtctatccg gttgccattt atcagtattt attggctaaa
      721 cagggaggca acaaattatt ggtatacatg cgccagagca gcatttaacg accctgactg
      781 gtttgtgaaa aaccaccacg cagttcgtca ggcaaagaga aaggccaata tgacatacat
      841 gaaggcgtat caaaaagcat ggaaagaaca ccgcgatcga taccaacaag acatggaaaa
      901 gcttgaatca gaaaacatgg aattaagacg aaagctcggt gaagcaaaac gagacattga
      961 tgcttacaag cgacttttta atggtgaaag ccatgcttag cccatcccaa tcccttcaat
     1021 accagaaaga aagcgtcgag cgggctttaa cgtgcgctaa ctgcggtcag aagctgcatg
     1081 tgctggaagt tcacgtgtgt gagcactgct gtgcagaact gatgagcgat ccgaatagct
     1141 caatgtacga ggaagaagac gatggctaaa ccagcgcgaa ggaaatgcaa aatatgcaag
     1201 gaatggtttc acccggcatt ctcaaatcag tggtggtgct gcccggaaca cggaactcag
     1261 ttagcactca aactacaaag taaacagcga aaaaaagcgg aaaaagcagc agagaagaaa
     1321 cgacgacgag aggagcagaa acagaaagat aaactgaaga ttcgaaaact cgccttaaag
     1381 ccccgcagtt actggattaa acaagcccaa caagccgtaa acgccttcat cagagaaaga
     1441 gaccgcgact taccatgtat ctcgtgcgga acgctcatgt ctgctcagtg ggatgccgga
     1501 cattaccgga caactgctgc ggcacctcaa ctccgatttg atgaacgcaa tattcacaag
     1561 caatgcgtgg tgtgcaacca gcataaaagc ggaaatctcg ttccctatcg cgtcgaactg
     1621 attaaccgaa tcgggcagga agcagtagac gaaatcgaat caaaccataa ccgccatcgc
     1681 tggactatcg aagagtgcag ggccatcaag gcgaagtatc agcagaaact taaagacctg
     1741 cgaaacagca gaagtgaggc cgcatgacgt tcacagtaaa aaccattcct gacatgctcg
     1801 ttgaggcata tgaaaatcag accgaggtag ccagaatact gaactgtagt cgcaacacgg
     1861 tcagaaaata cactggcgat aaagaaggaa aaagacacgc tattgtcaac ggtgttctta
     1921 tggttcatcg cggatggggt aaagatactg atgcgtgata tccggcaggt tcttgagtgc
     1981 tggggggcat gggcggcaaa taaccatgag gatgtgacct ggtcacccat tgccgccgga
     2041 tttaagggac tgatccccga aaaagtaaaa tcacgcccgc agtgctgtga cgatgatgcg
     2101 atggtgatat gcgggtgtat agcccgcctt taccggaaca atcgcgatct gcatgacttg
     2161 ctggttgatt attacgtgct gggggggacg ttcatggcg
//
LOCUS       DAZKIF010000310         2153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZKIF010000310 DAZKIF010000000
VERSION     DAZKIF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.312"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1439)
                     /locus_tag="ACHOTW_004992"
     CDS             complement(<1..1439)
                     /locus_tag="ACHOTW_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9846904.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSK"
     gene            complement(1414..1959)
                     /locus_tag="ACHOTW_004993"
     CDS             complement(1414..1959)
                     /locus_tag="ACHOTW_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9846905.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYECHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
BASE COUNT          500 a          617 c          512 g          524 t
ORIGIN      
        1 tttttcgagc gttcatacac aatggtcggg tcaatcccgc cagtatccca gcagatacgg
       61 gataccgaca tttctgcacc attccggcgg gtataggttt tattgatggc ctcatccaca
      121 cgcagcagcg tctgttcatc gtcgtggcgg cccataataa tctgccggtc aatcagccag
      181 ctttcctcac ccggccccca tccccatacg cgcatttcgt agcggtccag ctgggagtcg
      241 ataccggcgg tcaggtaagc cacacgatca ggaacggacg ctgaataatg ctctttccgc
      301 tctgccatca cttcagcatc cggacgttcg ccgattttcg cttcccacgt ctcaccgagc
      361 gtggtgttca cgaaggtttt acgttttccc gtatcccctt tcgtcttcat ccagtctttg
      421 acaatctgca cccaggtggt gaacgggctg tacgccgtcc agatgtgaaa ggtcacactg
      481 tcaggcggtt caatctcttc accggatgac gaaaaccaga gaatgccatc acgggtccag
      541 atcccggtct tttcgcagat ataacgggca tcagtaaagt ccagctcctg ctggcggatg
      601 acgcaggcat tatgctcgca gagataaaac acgctggagg ggtcatccgg cgtccatttg
      661 aggccaaacg gcgtctcttt gtcgccaaat ttaagatact gctcctcccc gcagtgcggg
      721 caggcaacat gaaaacgcat aaaatgcggg gattcactgg ctgcacgctc aatctggcag
      781 gtgcctctca ctttgggcgt ggagccacgg atggactttg gccagaccga gccttcaata
      841 cgcttgtcac ccaggaacgt cggagagcct tcctgttcaa tatcctcatc aaaggcagca
      901 agttcatcat aacccgccac atcaaccgac ttttcacggt agttttttgc cgctttaccg
      961 cccaggcacc agaagccacg accattggtg aaacgcttca tagtgagcgt gttatcccgg
     1021 tgctttttgc cataccacgg ggccagagac agcagcgacg gaatatcgcg gatggtaggc
     1081 tcgacgtggg ttttcataaa gttctcggca tcaccatccg tcggcaacca gataagggtg
     1141 ttacgctgct tatgctctat gaagtaggca taaacaccca gcagcatttt tgaataacca
     1201 acacgggcag acttcaccac attcacctcg cggatgtagt cgctgcccat cgcattcatg
     1261 atagcccgct gaaagggtag tgtttcccag cgcccttcct ggtatgcgga ttctttcggg
     1321 agatagtaac tggcatccgc ccattcaacg gcagtctgtg gctccggcct gaacagggct
     1381 cgcagcccgg cgcgtacatc acgccgcaga atatcaatct gactgttcga tatattcact
     1441 cagcaacccc ggtatcagtt catccagcgc ggctgctttg ttcatggctt tgataatatc
     1501 ccgtttcagg aaatcaacat gtcggttttc cagttccgga aaacgccgct gtactgagag
     1561 agggatcccg tcaagaatac tggcaatttc acctgcgatc cgtgacagca cgaaagtaca
     1621 gaatgcggtt tccaccactt ctgcggagtc tctggcattc ttcagctcct gggcgtcagc
     1681 ctgcgcacgc gtaagtcgat ggcattcgta ctcaatagtc ccaggctgga gatctgcctc
     1741 gctggcagcc ctgtaatcct caacctcttt acggagtttt tcattttcga tatcagcttc
     1801 cctctgcgca taccactgaa ttgccgtggc ggtatcaaat acagattcaa cgcccttacc
     1861 acctccggag acgcaaggga gcccctgaga ctgccagcgt tcaatcgttc gcggatccac
     1921 gttgaaaatt tcggcaagtt tctttttatt aaccttcatg aaacagtctc acaacaaata
     1981 cagggtccga catgaaagtg cccgaaaatg actttttcag gcgttttcat gtcggacctt
     2041 tacggattcg atattagaaa aaacaaatag ttatgttcga gaagtaccga catgattttc
     2101 cccggaaaat tttcataaat agcgaaaacc cgcgaggtcg ccgccccgta acc
//
LOCUS       DAZKIF010000311         2152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZKIF010000311 DAZKIF010000000
VERSION     DAZKIF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.313"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1336)
                     /locus_tag="ACHOTW_004994"
     CDS             complement(<1..1336)
                     /locus_tag="ACHOTW_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFP9846906.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDD"
     gene            complement(1333..2040)
                     /locus_tag="ACHOTW_004995"
     CDS             complement(1333..2040)
                     /locus_tag="ACHOTW_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9846907.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
BASE COUNT          590 a          514 c          481 g          567 t
ORIGIN      
        1 catcgtcaac gggcattggc tgcggttgcg gctccggttc aacagtttgc ttctcggtaa
       61 aattcgccgc ctgaattaat aatcgacgaa taattaatgc ttccagtgga caacgatttt
      121 ctgcatcaag acggcttacg atcggcatta atgattcttc tgtaggttgg ctggcctgca
      181 ctaattgttt ggcaactaga tataatggcc ataaggtatg ttctgtatca cgcgtgagtg
      241 agatccgcgg cttacatcct ttgtgcaaaa ccatctcgac cagtgaacgc cccagagcag
      301 agagtttttc atcgctgagt atttcgtata aatgaaaacg cgtacgggcg ctgtcgccgg
      361 ggacataaag tagcggtttt ccctggtcgt taaccagttc acgagcttca atcatcatca
      421 ccactaatgg gtaataatcg actcgatgct gacggcacca ttgcaacatt aaagcatccg
      481 cctgagtgga gggatactcg tgccagtagt cacaaagttc tgccagcagg ctttcgcctt
      541 ctccacaata gacgcgttgc gcgtattcat aataacgcgc atcttcatta tcaggttcag
      601 cttcccgcca cgaccgggcg taatccagga gttcggggat tcccaggcga cctgcactat
      661 accagcggag taaatttctg tgcagttttg catcatcggg gataatcact gcgcgatgtt
      721 gcgctaaata caatgtcagc cattctggat gcgatgacca aatccgggcg aacatttggt
      781 aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg cagaatgatc tggaagttaa
      841 aaacatttcc cttcgcaatg gcatataaaa aggattccag ttcttcttcg tctatttccc
      901 cgacgttttc ctcctgcaac caccgcaatc tttctgctaa taacaccacg cactcgaagg
      961 ataaatgggc agtgttcatg gctattgtgc acagcgacca gcgtaattca tcaatctcat
     1021 tcatcgagca ataatttaat tgctgaatga atcgctgcca ggcggagggc agaaaacgtt
     1081 cactatcaga ggcaagtaac gcacgaaagg cgagcagaat ttcatgttct gctacctcat
     1141 attcttctgg ttgccaaaca gatttagcag gcgactgcgc aatccggagc gcttcctcat
     1201 acgcctgacg aagttgttta aaaccctgcg gatcggtttc tggatgaaag gacggtaaca
     1261 gcgccagata agcgcggcgg ataatatcga catcagtcgt ttcctctatg ttgaggatct
     1321 tccagcaatt tttcataggt cgctcatatc cttaagcgtt tccgggcatg tggggtagtc
     1381 agccagtggt tgccagggta aggtcgcgta atatgcatcg tcactggcaa ataattgata
     1441 aaacatatca ttgccggaat cgtcgtattc tttacgcgct aagaattcac gtaataaagg
     1501 cagattatca tcttcgggag actgccaata ttgccgacca aaggtataac cggcaaagta
     1561 ttgccgccag ttactataat aacgtagagc ccgtagatga atacgcgact ggatccatag
     1621 actttcttct tcactcaacc agttattaag tacgcccatt cgacttaaaa aacccatccg
     1681 gacataatcc catgctttaa ttcctccctc accacagcat tcggctgtgc tggcgacaaa
     1741 ttgcgcataa gcctgacctt gctcatttaa ttcagcaaga tagtcacgcc attcacatgg
     1801 agaataacga aaccagcggt gataaaaccc tgccaaccgc gcggcatgac cgttatcgat
     1861 cattcggtga attgtgcgca gcaggtcatc acggtcatca attccccagg aggaagaaag
     1921 gtcaaaaaag cgtgtaccta aatacatttt gggtgatgaa tgcgaaggta aacagcattc
     1981 attgcgtatt tccatcggag tggaaagtgc aaacaatatt ttttgcgact ccatgtccat
     2041 atgatttatc cttaaatatt cattgaatga atattagccg cattgcgacg ctcgtttatt
     2101 tatacttttc tgacgcgaca taatacgcat aaaataaggc ttgcgcaaaa aa
//
LOCUS       DAZKIF010000312         2117 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZKIF010000312 DAZKIF010000000
VERSION     DAZKIF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.314"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1055
                     /gene="escC"
                     /locus_tag="ACHOTW_004996"
     CDS             <1..1055
                     /gene="escC"
                     /locus_tag="ACHOTW_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFP9846908.1"
                     /translation="SESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPG
                     VVSILKTMASNGSLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQ
                     LISELDIEQRQIEISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSS
                     VISNASNFMIRVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLE
                     SITSGTLLRVTPRILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEV
                     QNSEMTTEATLSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVV
                     RLFLIKATPIKSASSE"
     gene            1069..1524
                     /gene="cesD"
                     /locus_tag="ACHOTW_004997"
     CDS             1069..1524
                     /gene="cesD"
                     /locus_tag="ACHOTW_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFP9846909.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(1908..>2117)
                     /gene="grlA"
                     /locus_tag="ACHOTW_004998"
     CDS             complement(1908..>2117)
                     /gene="grlA"
                     /locus_tag="ACHOTW_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFP9846910.1"
                     /translation="ISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPGTTGPKRKT
                     YRVGNGIVGQSNIWNEMIMRRKKES"
BASE COUNT          697 a          413 c          391 g          616 t
ORIGIN      
        1 tatcagaaag tcttgataaa gaggcacagt ctaaagccaa aaataaagat gttgttaagg
       61 tctttaaact gaactatgcg tcagcgacag atataacata caaataccgt gaccaaaatg
      121 ttgtcgtccc aggtgttgtc tccattctga agacaatggc aagtaatggt tcacttccca
      181 gtaccggtaa aggtgccgtc gagcgaagcg gtaacctgtt tgataacagt gtaacgattt
      241 ccgctgatcc acgtcttaat gcagttgttg taaaagaccg cgaaataacg atggatatct
      301 atcagcaatt aatttctgag cttgatattg aacaaaggca aattgaaatt tctgtctcaa
      361 ttattgatgt tgatgcaaac gatcttcagc agctcggcgt aaactggtcc ggtacgttaa
      421 atgcaggaca aggtacgatt gcctttaata gctctaccgc acaggctaat atcagctctt
      481 ctgtgataag taatgccagt aattttatga ttagggtgaa tgcgttacaa cagaactcga
      541 aagctaaaat actctctcag ccttcaatta taaccttaaa taatatgcag gcgattcttg
      601 ataaaaacgt aacgttttat acaaaagtct ccggagaaaa ggtggcgtct ctggaaagta
      661 ttacaagcgg aacattgctg agagtgacac cacgtatact tgatgacagc tcgaatagcc
      721 ttaccggaaa acgtcgggaa cgagttcgtt tgttacttga tattcaggat ggcaatcaat
      781 cgactaacca aagcaatgct caggatgcca gcagcacctt acctgaagta caaaattcag
      841 aaatgacaac agaagcgaca ttaagtgcag gagaaagttt attacttggc ggatttatcc
      901 aagataaaga aagttcaagt aaagatggta taccgctgtt aagcgacatt cctgttattg
      961 gtagtttatt ctccagtacg gtaaaacaaa aacatagtgt tgttcgactc tttttaatta
     1021 aagcaacccc gataaaatct gcatctagcg aataataaat gaggctttat gagcaggaaa
     1081 tttagctctc tagaggatat ttatgatttc taccaggatg gtggcacatt agcgtcatta
     1141 acaaatctga cacaacaaga tctcaatgac cttcattctt atgcctatac agcatatcaa
     1201 tctggtgatg ttataaccgc aagaaatcta ttccatttgc tcacatatct ggaacactgg
     1261 aattatgact acaccttatc tctgggctta tgtcatcagc gtttatcaaa tcatgaagat
     1321 gcacaactgt gtttcgcacg ctgtgcaact ttagttatgc aagatcccag ggcatcttat
     1381 tattctggaa ttagctactt actcgtcgga aataagaaaa tggccaagaa agcctttaag
     1441 gcttgtttaa tgtggtgtaa tgaaaaagaa aaatacacta catataaaga aaatattaaa
     1501 aaattgttag gtaatacaga gtaaaaacta ccatcagcta taccaatccc caggctgact
     1561 ggggattttc tatcggaaat aataaagctg ctatagtgaa gtgctcccaa agttgagcac
     1621 cgaccactgg tagatttatc tacaggtgtg gggttattcc attaaccagg agtttatgcc
     1681 ctgaactatc ttctgctgta tttaatatca tttccgttta tgaaacaatc agtatatctt
     1741 aacttagcat tagaaatctg atctttccaa tcatctatag ctcagagtat atttgtcagg
     1801 aaatgacctt caaactttag ccgagaaaaa ggcttaccct ggaaaacaaa acccttaaat
     1861 atagctttat ttttattctt ctataaaata tactcaaaaa attacgtcta actctccttt
     1921 ttccgcctca tgatcatttc gttccagata ttagactgtc ccacaatacc attaccaact
     1981 cggtaggttt ttctttttgg tccggttgtt ccaggacttt ctatcggaac cgcctcaagg
     2041 ttaacatcat aaatgaaaat ctcaaggcgc ttataatgca aattaccata actaacatat
     2101 ctgacacgaa atgaaat
//
LOCUS       DAZKIF010000313         2058 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZKIF010000313 DAZKIF010000000
VERSION     DAZKIF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.315"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1078
                     /gene="rfaL"
                     /locus_tag="ACHOTW_004999"
     CDS             <1..1078
                     /gene="rfaL"
                     /locus_tag="ACHOTW_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFP9846911.1"
                     /translation="LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSP
                     DAGASMKEIFKAIIENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELIT
                     YYKDYQQGIMPFADYRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLIL
                     GTLSRGAWLSVLVIGLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQ
                     TNSSYRYANGTQGSALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHN
                     FALFIWFGTGLLGLVSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIR
                     GNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(1114..>2058)
                     /locus_tag="ACHOTW_005000"
     CDS             complement(1114..>2058)
                     /locus_tag="ACHOTW_005000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9846912.1"
                     /translation="RIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMK
                     LYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPN
                     GIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLK
                     LVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPS
                     QFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTL
                     ANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          622 a          385 c          358 g          693 t
ORIGIN      
        1 tcttatgaca ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact
       61 atttaaaaca tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact
      121 tcttcaatcc cctgatgcag gtgctagcat gaaggaaatt ttcaaagcta ttattgagaa
      181 tactttacta tgcacaatag caataccgat catattgaga gacgagaaaa gagaagatgt
      241 cgaaaaaatc gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt
      301 gattacctat tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg
      361 tagcatttct gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa
      421 atcagcaaaa taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt
      481 aggaacttta tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct
      541 gatgtttaaa caatggaagt tactattagt aggagtaatg gttgccatca ttgcattgtc
      601 ggttattttc acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa
      661 tagttcttat cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa
      721 tcctgttatt ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt
      781 cattgattat ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt
      841 catctggttt ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat
      901 attgaaagag tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata
      961 ttatataatc ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat
     1021 tgaaccaaat ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa
     1081 aataaaaaag gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc
     1141 ttcgaatctt tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg
     1201 ctttgcaact gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga
     1261 gtctgcagtc ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt
     1321 catcccccca cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac
     1381 catgcaaaat ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata
     1441 acaatgcatc ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt
     1501 tgcaatttcc ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata
     1561 atcaccaatc acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg
     1621 catcaacagc aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc
     1681 ttcaggggag atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc
     1741 taaatcaatt ccatttggta caatctcaat atctgcattt gctaaataag attggtaata
     1801 ctttttcaag tataaactag gcactatcat tttaacattt tgctcaagca attttggttc
     1861 aaatgcatta tgcatatgta taaccactct tgcctgagga gctcgcttac gaatttgagt
     1921 gtatagcttc atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg
     1981 tgcaatattc aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa
     2041 taaccgctta tatatgcg
//
LOCUS       DAZKIF010000314         2010 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZKIF010000314 DAZKIF010000000
VERSION     DAZKIF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.316"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            52..597
                     /locus_tag="ACHOTW_005001"
     CDS             52..597
                     /locus_tag="ACHOTW_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP9846913.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            572..>2010
                     /locus_tag="ACHOTW_005002"
     CDS             572..>2010
                     /locus_tag="ACHOTW_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9846914.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSK"
BASE COUNT          482 a          487 c          593 g          448 t
ORIGIN      
        1 ctttttggcc tctgtcgttt cctttctctg tttttgtccg tggaatgaac aatggaagtc
       61 aacaaaaagc agctggctga cattttcggc gcgagtatcc gtaccattca gaactggcag
      121 gagcagggaa tgcccgttct gcggggtggt ggcaagggta atgaggtact ttatgactct
      181 gccgccgcca taaaatggta tgccgaaagg gatgctgaaa ttgagaacga aaagctgcgc
      241 cgggaagttg aagaactgag gcaggacagc gagacagatc tccagccagg gactattgag
      301 tacgaacgcc atcgacttac gcgtgcgcag gccgacgcac aggaactgaa gaatgccaga
      361 gactcagctg aagtggtgga aaccgcattc tgtactttcg tgctgtcgcg gatcgcaggt
      421 gaaattgcca gtattctcga cgggatcccc ctgtcggtgc agcggcgttt tccggaactg
      481 gaaaaccgac atgttgattt cctgaaacgg gatatcatca aagccatgaa caaagcagcc
      541 gcgctggatg aactgatacc ggggttgctg agtgaatata tcgaacagtc aggttaacag
      601 gctgcggcat tttgtccgcg ccgggcttcg ctcactgttc aggccggagc cacagaccgc
      661 cgttgaatgg gcggatgcca gttactatct cccgaaagaa tccgcatacc aggaagggcg
      721 ctgggaaaca ctgccctttc agcgggccat catgaatgcg atgggcagtg actacatccg
      781 cgaggtgaat gtggtgaagt ctgcccgtgt tggttattcc aaaatgctgc tgggtgttta
      841 tgcctacttc atagagcata agcagcgcaa cacccttatc tggttgccga cggatggtga
      901 tgccgagaac tttatgaaaa cccacgttga gccgactatt cgtgatattc cgtcgctgct
      961 ggcgctggcc ccgtggtatg gcaaaaagca ccgggataac acgctcacca tgaagcgttt
     1021 caccaatggg cgtggcttct ggtgcctggg cggtaaagcg gcaaaaaact accgtgaaaa
     1081 gtcagtggat gtggcgggtt atgatgaact tgctgccttt gatgaggata ttgaacagga
     1141 aggctctccg acgttcctgg gcgataagcg tattgaaggc tcggtctggc caaagtccat
     1201 ccgtggctcc acgcccaaag tgagaggcac ctgccagatt gagcgtgcag ccagtgaatc
     1261 cccgcatttt atgcgttttc atgttgcctg cccgcactgc ggggaggagc agtaccttaa
     1321 atttggcgac aaagagacgc cgtttggcct caaatggacg ccggatgatc cctccagcgt
     1381 gttttatctc tgcgagcata atgcctgcgt catccgtcag caggagctgg actttactga
     1441 tgcccgttat atctgcgaaa agaccgggat ctggacccgt gatggcattc tctggttttc
     1501 gtcatccggt gaagagattg aaccgcctga cagtgtgacc tttcacatct ggacggcgta
     1561 cagcccgttc accacctggg ttcagattgt caaagactgg atgaagacga aaggggatac
     1621 gggaaaacgt aaaaccttcg tgaacaccac gctcggtgag acatgggaag cgaagatcgg
     1681 cgaacgtccg gatgctgaag tgatggcaga gcggaaagag cattattcag cgtccgttcc
     1741 tgaccgtgtg gcttacctga ccgccggtat cgactcccag ctggatcgct acgaaatgcg
     1801 cgtatgggga tgggggccgg gtgaggaaag ctggctgatt gatcggcaga ttattatggg
     1861 ccgccacgac gatgaacaga cgctgctgcg tgtggatgag gccatcaata aaacctatac
     1921 ccgccggaat ggtgcagaaa tgtcggtatc ccgtatctgc tgggatactg gcgggattga
     1981 cccgactatt gtgtatgaac gctcgaaaaa
//
LOCUS       DAZKIF010000315         2001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZKIF010000315 DAZKIF010000000
VERSION     DAZKIF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.317"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..79)
                     /locus_tag="ACHOTW_005003"
     CDS             complement(<1..79)
                     /locus_tag="ACHOTW_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9846915.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTS"
     gene            complement(91..489)
                     /locus_tag="ACHOTW_005004"
     CDS             complement(91..489)
                     /locus_tag="ACHOTW_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9846916.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(531..1556)
                     /locus_tag="ACHOTW_005005"
     CDS             complement(531..1556)
                     /locus_tag="ACHOTW_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9846917.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(1612..1944)
                     /locus_tag="ACHOTW_005006"
     CDS             complement(1612..1944)
                     /locus_tag="ACHOTW_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9846918.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
BASE COUNT          420 a          593 c          540 g          448 t
ORIGIN      
        1 ctgacgttcc catgtacccg cgtatcgttt catcggcgcg ggcaatggca gcatcgaaca
       61 ggttatcgaa atcagccaca gcgcctcccg ttattgcatt ctggccaggc cgcgctctgt
      121 catttcggct gccacaccgg cagagacacg aaacgccgtt cccggcagca caaatgccac
      181 aggttcatcc cgcgtgacgt gaagtgcatc agtatgcagc ttcaccagtg ccacgaccgt
      241 gaccagttca gacgtatcca gaatcacggt atccggctgc gctgatccca cctcattttc
      301 atgtccggtc agcacatttt cccggctgag aggggtgtcc tgaccggcag tttcatccgt
      361 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
      421 caggctgaca tcacggttca gttgttcacc cagcgagcgg agacgggcaa tcagttcatc
      481 tttcgtcatg gactcctcca cagaaaaaca atggccccga agggccatga ttacgccagt
      541 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
      601 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtaacg ggcagaggcg
      661 ttaatgcctt cgcgctgtgc gtccgcatcc tgaatgcagc cataggtgcg cagaccgcgt
      721 gcctgagtgt tccccagcac catcgtgttg tccggcagga agttcttttt gacgccgttt
      781 tccacgtact gtccggaata cacgacgatg gccacatcgc catacattcc cttataagac
      841 accgctttgc ccaggtcttt taccgctgtc tccagttcgg aatgagagcc gcgacgggta
      901 tccagcttct ccctgacggc tttgaaggaa cggaacagcg cccagccttt cgggtcaaac
      961 acgatgatat tcaccacgcc gctggcgttc agcgcgtagg cttcgatatc gtcggtcggg
     1021 tcatacgtgg acttgtcacg cttgctccac tccgtgccgc cggactgcgt gatgttgttc
     1081 gccgcactgc ggcccatatc cacctcaacc ggatcgaagg cttcaccggt catggtgtat
     1141 ttgcccttga gcacggcaga aactgcctgc atctcttcga cctgagcaat ggccagctct
     1201 tcgtcacgca tgttctgcat gatgatgcga cggcggcggt aagccgggtc cgccagattc
     1261 tgcggatctt catccggcag gcgacgcagg gtcatctgcg gattcacttc atgcttcggc
     1321 ttgacatatc ccggcgtaaa ttcagaggtg gagccgccac gggaacggat aacctcacca
     1381 gaaacaatcg gcgaaacgta cagcgccatg tttaccagtc ccggaatttg tgagagatag
     1441 actttctccg tggtgaaggg atagctctca cggaaaaaga gacgcagaaa cagcggatca
     1501 aacttaaatt tctgctcatt tgccgccagc agttgggcgg ttgtgtacat cgacataaaa
     1561 aaatcccgta aaaaaagccg cacaggcggc ctttagtgat gaagggtcag gttaaacgat
     1621 gctgattgcc gttccggcaa acgcggtccg ttttttcgtc tcgtcgctgg cagcctccgg
     1681 ccagagcaca tcctcataac ggaacgtgcc ggacttgtag aacgtcagcg tggtgctggt
     1741 ctggtcagca gcaaccgcaa gaatgccaac ggcagcaccg tcggtggtgc catcccacgc
     1801 aaccagctta cggctggagg tgtccagcat cagcggggtc attgcaggcg ctttcgcact
     1861 caatccgccg ggcgcggttg cggtatgagc cgggtcactg ttgcccagcg gctggtaatg
     1921 ggtaaaggtt tctttgctcg tcataaacat cccttacact ggtgtgttca gcaaatcgtt
     1981 aacggcatca gatgccgggt t
//
LOCUS       DAZKIF010000316         1944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZKIF010000316 DAZKIF010000000
VERSION     DAZKIF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.319"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..543)
                     /locus_tag="ACHOTW_005007"
     CDS             complement(<1..543)
                     /locus_tag="ACHOTW_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9846919.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQ"
     gene            812..928
                     /locus_tag="ACHOTW_005008"
                     /pseudo
     CDS             812..928
                     /locus_tag="ACHOTW_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1033..1109)
                     /locus_tag="ACHOTW_005009"
     tRNA            complement(1033..1109)
                     /locus_tag="ACHOTW_005009"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1073..1075),aa:Arg,seq:tct)
     gene            complement(1123..1199)
                     /locus_tag="ACHOTW_005010"
     tRNA            complement(1123..1199)
                     /locus_tag="ACHOTW_005010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1163..1165),aa:Arg,seq:tcg)
     gene            complement(1207..1282)
                     /locus_tag="ACHOTW_005011"
     tRNA            complement(1207..1282)
                     /locus_tag="ACHOTW_005011"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1247..1249),aa:Ile,seq:cat)
     gene            complement(1311..>1944)
                     /locus_tag="ACHOTW_005012"
     CDS             complement(1311..>1944)
                     /locus_tag="ACHOTW_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9846920.1"
                     /translation="GSVVLATKCIRALFLMHYGKVKIFDINHSIVSQYLEIQHKLTRT
                     HLTDVPLYLSLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLFESDERILLFLSGC
                     LSSISAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMRV
                     AVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          505 a          502 c          470 g          467 t
ORIGIN      
        1 ctgggtgaat gcagaaccac cacgacagca tggtaccagc aggatccccg cgttattcgg
       61 gatatacgga agcagttttt tggcaatatg taagccctgg ccgacacagc cgtactgccc
      121 tttgctcagg tctgccttcg gatgattcag cgtactcata tcctgcacat catgcaggca
      181 gtggtcggcc ggaatgatgt cgttatacgt acaactctct ccacccggag ttaccgtgct
      241 gcggcgcgcc agctgtttaa tgcgcggatc cggagcatcg taagaatccg gtaacggaag
      301 cccttcaccg taggccatgc cgttggactg tccggcaagc acaaccacgt agaaccagtc
      361 cggcttagat gaagggccga cctgtggatc tccttcaata gccaccgcct gcatcagtgt
      421 gtacggcgta atggcaaccg gtccgccgta tggctgccag ccctctttca gtttgtgtgt
      481 cagcttttcc gcaaggtctg acggcgacgc cgccctgaca acatcatagt gtttaaatgc
      541 catgaatcct cccggccggg ataatattgt gagtaaaatg aggagcgggc tgaagtccgg
      601 aagttacagg acaatggcag aagagagaca acagcccgca atacgaaaaa ggccgcgcta
      661 ttgcgcagag tgattactgt cggatattat tcgccagctg aaatattact tcacgttttg
      721 ttgtttattc cttgccgccc gcgtctccct gcgcgggctt tttttgtcca taagaaagcc
      781 cctccggaga ggggctggag agtggcgcta tgtgccattg catggtgccg ggtgcctccc
      841 ggtgaattca gtaccagcac ctgaatccgc gattatccca tatacctact cgctgattgc
      901 ccctccgcac agggggattc accatgccag tttcttttaa caaactcccc gcaaaccaga
      961 caacagtcaa ccgcctgaat tgtgagacat ttaaaaaaaa ggcccgcaaa agcgagccag
     1021 ggaaaataag tgtggcgcgt tgtactggat tcgaaccagt gaccgattgc ttagaaggca
     1081 attgctctgt ccggctgagc taacaacgca ggatacagat aatggaccgc cttcggggac
     1141 ccgaactccg cgcaaccagc ttcgaaagct ggcgctcttt cctgatgagc taatggcggt
     1201 atgtgatggt ggcccttgct ggatttgaac cagcgacctg gcgattatga gtcgctcgct
     1261 ctcaccactg agctaaaggg ctgggtcaaa aaataataat cagatgaaaa tcaataatca
     1321 agcccttgcc tggatacata tctgtctggc gggaagccat aatagcggtg aaatacagaa
     1381 ataaagtagg accagcttga ataaccgcat ttttctgcta cagcctgtcc atatccatgc
     1441 cgggaacata acatattgac agcaacacgc atccgctctt ccagcaacaa gcgactgaac
     1501 atgtgccctt catttttcag ttttatcttt aacaaactct cactcatatg caggcgtaac
     1561 gcaatcgcac caagcgtcca gcttgctgat atatctgtct gaattatcgc cctgactttg
     1621 gcacttatac tggataaaca tccacttaaa aataataata tccgttcatc tgattcaaac
     1681 agcgacaggc atgccatcat aagaaacata tccgtggtat ctccggaaaa tctctggctg
     1741 gtaattaaag cctcagccaa cgcagggttg ttgggttcca gtgacagata aagcggaacg
     1801 tcagtcagat gagttcttgt cagcttatgc tgaatttcca gatattgact tactatggaa
     1861 tggtttatat cgaaaatttt aactttgcca taatgcataa ggaaaagcgc cctgatgcat
     1921 ttggtggcca gaacgactga gccg
//
LOCUS       DAZKIF010000317         1923 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZKIF010000317 DAZKIF010000000
VERSION     DAZKIF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.320"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1674
                     /locus_tag="ACHOTW_005013"
     CDS             <1..1674
                     /locus_tag="ACHOTW_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9846921.1"
                     /translation="AHFDGDQSGTLNSVIPPAVQHLTVEVSAADSQYLAQAKWDTPRV
                     VKGVRFSLRLTSGSGQDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPA
                     ITTFRINAPAKPATIELTPGYFQITAVPVLAVYDPTVQFEFWFSEKRITNTAQVEKSA
                     RYLGSGSQWTVQGSRIKPGTDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREK
                     IGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEITQTVSKTLENQSATIQQIQRV
                     QKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPES
                     GNSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPDGRLTAKNADISGSVN
                     ANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQK
                     FDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSS
                     VIDMPAGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            1742..>1923
                     /locus_tag="ACHOTW_005014"
     CDS             1742..>1923
                     /locus_tag="ACHOTW_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9846922.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTSAPG
                     SDNLNGINVKYRYEFT"
BASE COUNT          495 a          475 c          559 g          394 t
ORIGIN      
        1 gcgcactttg acggcgacca gagcggcacc ctgaacagcg tcatccctcc ggcagtgcag
       61 cacctgacgg tggaggtgag tgcagctgac agccagtatc tggcgcaggc gaaatgggac
      121 acgccgcggg tggtgaaggg cgtgcgcttc agtctgcgcc tgaccagtgg aagcggtcag
      181 gacagccgtc tggtgaccac cgccatcact gcggatacag agcatcgttt cagtggtctg
      241 ccgctcgggg aatacaccct gacagtcagg gcaattaaca gttatggcca gcagggggaa
      301 ccggccatca ccaccttccg gattaacgcg ccagcaaaac ccgccaccat tgaactgacg
      361 ccggggtatt ttcagataac ggcggtaccg gtgctggcgg tgtatgaccc gacggtgcag
      421 tttgagttct ggttttcgga aaaacgcatc acgaacacgg cacaggtgga aaaatctgcc
      481 cgttatctgg ggagcggcag tcagtggact gtccagggaa gccggattaa gccggggacg
      541 gatttctggt tttacgtgcg cagcgtcaac ctggtgggga aatctgcgtt tgtggaagtc
      601 agcgggcagc ccagcaatga tggtgaaggg tatctggaat ttttccggga aaaaatagga
      661 aaactgcatc tggctcaggg gctatgggag ctgatagaca acagccagct tgcggatgag
      721 atggcggaga tgaagaccac catcacggaa acccgcaatg aaatcacaca gacggtcagt
      781 aaaacgctgg agaaccagag cgccactata cagcagatac agcgcgtgca gaaggacaca
      841 aatgatgacc tggctgcgct gtacatgctg aaggttcaaa aaacgaaaga cggcattccc
      901 tatgtggccg ggattggtgc agggattgag gatactgatg gccagccact gagcaacata
      961 ctgctgctgg ctgaccgtat cgcgatgata aatccggaga gcggcaacag cacgccgtta
     1021 tttgtggcgc aggggaatca gctgttcatg aacgacgtgt tcctgaagcg actgtttgcg
     1081 gtgagtatca cctcgtccgg caatcccccg acgttttccc tgacgccgga cgggcgactg
     1141 acggcgaaaa atgcggatat cagtggcagt gtgaatgcga actcagggac gctcaacaac
     1201 gtcacgatta atgagaactg tcagattaag gggaaactgt cagccaacca gattgaaggc
     1261 gatattgtca aaacggtcag caagtctttc ccccgcacga gcacttatgc cagtggcacc
     1321 atcacggtaa gaatcagtga tgatcagaag tttgaccggc aggtcatgat accgccagtg
     1381 ttattccgcg gtggtaagca tgagaatttc aacagtaata accaacagtc atactggtat
     1441 tcaacctgcc ggttaagagt gacccgcaat ggtcaggaga tttttaatca gtccacgacg
     1501 gatgctcagg gcgtattttc ctcagttata gatatgcctg ccggacaggg gacgctgaca
     1561 ctgacattca ccgtatcttc atcaggagcg aataactgga caccaacaac cagtatcagc
     1621 gatctgctgg ttgtggtgat gaaaaaatcc acagcaggta tcagtatcag ctgaatttta
     1681 taacccatat acgggcgcca gaaatggcgc cttttttatt gcagaaaagc gagaggtaat
     1741 tatgcgtaaa ctttatgccg ccattttgtc cgcagccatc tgtctggccg tatccggcgc
     1801 gcctgcatgg gcgtctgagc agcaggccac gctgagcgcg gggtatcttc atgcccggac
     1861 gagcgctccc ggtagcgata atcttaacgg gattaacgtg aaataccgtt atgaatttac
     1921 gga
//
LOCUS       DAZKIF010000318         1886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZKIF010000318 DAZKIF010000000
VERSION     DAZKIF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.321"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            174..1856
                     /gene="tir"
                     /locus_tag="ACHOTW_005015"
     CDS             174..1856
                     /gene="tir"
                     /locus_tag="ACHOTW_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFP9846923.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQT
                     STSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATE
                     TATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAI
                     ENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQ
                     TTTTTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDT
                     SSIGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGAR
                     LLGNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV"
BASE COUNT          530 a          402 c          481 g          473 t
ORIGIN      
        1 cccgatgtgt gtttttagca cattattttc ttgcatcaaa aatcagattg tgatttattt
       61 ggtcgatgtc atgttcagcc gtttatcgac tacgtgctgt ataccataca ttattatgta
      121 atagcaaaat aaaggattgt tatctattta cataaaataa aaaggagata tttatgccta
      181 ttggtaatct tggtcataat cccaatgtga ataattcaat tcctcctgca cctccattac
      241 cttcacaaac cgacggtgca ggggggcgtg gtcagctcat taactctacg gggccgttgg
      301 gatctcgtgc gctatttacg cctgtaagga attctatggc tgattctggc gacaatcgtg
      361 ccagtgatgt tcctggactt cctgtaaatc cgatgcgcct ggcggcgtct gagataacac
      421 tgaatgatgg atttgaagtt cttcatgatc atggtccgct cgatactctt aacaggcaga
      481 ttggctcttc ggtatttcga gttgaaactc aggaagatgg taaacatatt gctgtcggtc
      541 agaggaatgg tgttgagacc tctgttgttt taagtgatca agagtacgct cgcttgcagt
      601 ccattgatcc tgaaggtaaa gacaaatttg tatttactgg aggccgtggt ggtgctgggc
      661 atgctatggt caccgttgct tcagatatca cggaagcccg ccaaaggata ctggagctgt
      721 tagagcccaa agggaccggg gagtccaaag gtgctgggga gtcaaaaggc gttggggagt
      781 tgagggagtc aaatagcggt gcggaaaaca ccacagaaac tcagacctca acctcaactt
      841 ccagccttcg ttcagatcct aaactttggt tggcgttggg gactgttgct acaggtctga
      901 tagggttggc ggcgacgggt attgtacagg cgcttgcatt gacgccggag ccggatagcc
      961 caaccacgac cgaccctgat gcagctgcaa gtgcaactga aactgcgaca agagatcagt
     1021 taacgaaaga agcgttccag aacccagata atcaaaaagt taatatcgat gagctcggaa
     1081 atgcgattcc gtcaggggta ttgaaagatg atgttgttgc gaatatagaa gagcaggcta
     1141 aagcagcagg cgaagaggcc aaacagcaag ccattgaaaa taatgctcag gcgcaaaaaa
     1201 aatatgatga acaacaagct aaacgccagg aggagctgaa agtttcatcg ggggctggct
     1261 acggtcttag tggcgcattg attcttggtg ggggaattgg tgttgccgtc accgctgcgc
     1321 ttcatcgaaa aaatcagccg gtagaacaaa caacaacaac aactactaca actacaacta
     1381 caactacaag cgcacgtacg gtagagaata agcctgcaaa taatacacct gcacagggca
     1441 atgtagatac ccctgggtca gaagatacca tggagagcag acgtagctcg atggctagca
     1501 cctcgtcgac tttctttgac acttccagca tagggaccgt gcagaatccg tatgctgatg
     1561 ttaaaacatc gctgcatgat tcgcaggtgc cgacttctaa ttctaatacg tctgttcaga
     1621 atatggggaa tacagattct gttgtatata gcaccattca acatcctccc cgggatacta
     1681 ctgataacgg cgcacggtta ttaggaaatc caagtgcggg gattcaaagc acttatgcgc
     1741 gtctggcgct aagtggtgga ttacgccatg acatgggagg attaacgggg gggagtaata
     1801 gcgctgtgaa tacttcgaat aacccaccag cgccgggatc ccatcgtttc gtctaaatat
     1861 atccataatc attttattta gaggga
//
LOCUS       DAZKIF010000319         1854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZKIF010000319 DAZKIF010000000
VERSION     DAZKIF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.322"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..77)
                     /locus_tag="ACHOTW_005016"
     CDS             complement(<1..77)
                     /locus_tag="ACHOTW_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846924.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGF"
     gene            complement(61..>1854)
                     /locus_tag="ACHOTW_005017"
     CDS             complement(61..>1854)
                     /locus_tag="ACHOTW_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9846925.1"
                     /translation="VSPCGERAFVILPWLHRETARSFGGAGSTAGYEQVTAYTLTGQL
                     QSRHLNLPQLDCDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPY
                     ATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLTEKTDRIPEGVIRM
                     HDERTHHYHYDNQHRLVFYTRIQYGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMS
                     LSRKPEVTWYGWDGDRLTTVQTGTTRIQTVYRPGSFTPLIRIETENGEREKAQRRSLA
                     EKLQQEGSEDGHGVVFPAELVRMLDRLEEEIRADRVSSESRAWLAQCGLTVEQLEKQV
                     EPEYTPARTLHLYHCEHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRL
                     PGQQHDEESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDA
                     IENMTSGGLIYAVSGVPGLIVANSITNSAYQFGYDMDAIVGGAHNGAADAMRYCYLMC
                     RMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIE
                     KYNIGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          355 a          581 c          454 g          464 t
ORIGIN      
        1 tcaaacccaa ggtaaacaac aaataaacaa agggtaatta aaaaaatgac aagtttgcct
       61 ctaataattt gaagcatctg aactcccttg ctttgctttt attggattat ctgcttttat
      121 accatctaac ccgaagagtt gcccaatgtt atatttttca atacatgcat cgctacattt
      181 ggcagaaaaa tcgccacaag caataccgac agtgttattt ttaagatcca tgattctttc
      241 tttagctggc tgaccttgtc tatccccagc cgcctcatga tttttaccta tcacgtcagc
      301 tattgttgat ccaaatgtct tagtcattcg acacatcaag taacaatatc tcattgcatc
      361 ggcggcccca ttatgagctc cgccaacaat agcatccata tcataaccga actggtaagc
      421 actgttagta atgctgtttg caacaatcaa tccaggtaca ccagatacgg cataaattag
      481 tccacctgat gtcatattct caatcgcatc taaccccatt gggtctatca cttgtatggg
      541 attcaacgga tactgataca tattccatcc acctctcaac ccaatcgggt ccggggtgat
      601 ataccgcccc tgcaacggat cgtagtaccg gtgacgatta tagtacagcc ccgactcctc
      661 atcatgctgc tggcccggca gacggtacgg ctggtgcagg tgatgcgggt tctcctcatt
      721 aagctggttg ccccattcat catattccgc gctccacgcc gtattgccgt cttcgctgat
      781 aagcgccagc ggcagtcccc ggtgctcaca gtggtacaga tgcagcgtgc gcgccggcgt
      841 gtattccggc tccacctgtt tttccagttg ctccaccgtc agtccgcact gcgcaagcca
      901 cgcccggctt tcactgctca cgcggtctgc ccggatttct tcctccagcc tgtccagcat
      961 cctcaccagt tctgccggga acaccacacc gtgaccgtcc tcactccctt cctgctggag
     1021 tttctccgcc aggctgcggc gctgcgcttt ctcccgctcg ccgttctccg tttcgatgcg
     1081 gatgagcggt gtgaagctcc ccggccggta taccgtctgg atacgtgtgg tgccggtctg
     1141 taccgtcgtc agcctgtcgc cgtcccaccc gtaccaggtc acctccggtt tacgcgacag
     1201 cgacatccac cccgtcaggt cacgctcccg ccgccacacc cgtttccccg tccggcggcc
     1261 cagcgggtca tagaggtagc ggctctcgac cagcggctcg ccgtattgta tccgcgtgta
     1321 gaacaccagg cggtgctggt tgtcgtaatg gtagtggtgg gtccgctcgt cgtgcatccg
     1381 gataacccct tccggaatgc ggtccgtctt ctccgtcagc ctgccgtatt catcgtagcg
     1441 gtagacatag tgcgcatctt ccgcgatgcg gttatccggc cacgccgtga gcgtgctgtc
     1501 cggatgcagt tccgggtccg gcagccggtt tcctgccggg tccgttgcat acgggatatc
     1561 gatatccagg ttcgctgccg tggtgtggac gcccgtcagc cttcccgtgt cgctgtaacg
     1621 gtactcccgg ctctcctgcg ggccgctgat gcggattagc tgtccgttgt cgttccagtc
     1681 gtagtcacag tcaagctgcg ggaggttcag gtgcctgctc tgtagctgcc ctgtgagggt
     1741 gtacgccgtg acctgttcgt accccgctgt actgcccgcc ccgccgaagc tgcgggccgt
     1801 ctcacggtgc agccagggca agattacgaa agcccgctcc ccgcaaggac tgac
//
LOCUS       DAZKIF010000320         1807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZKIF010000320 DAZKIF010000000
VERSION     DAZKIF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.323"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..132
                     /locus_tag="ACHOTW_005018"
     CDS             <1..132
                     /locus_tag="ACHOTW_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein O"
                     /protein_id="HFP9846926.1"
                     /translation="SPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWIYGVDL"
     gene            129..830
                     /locus_tag="ACHOTW_005019"
     CDS             129..830
                     /locus_tag="ACHOTW_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9846927.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNEVNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGASV"
     gene            827..1117
                     /locus_tag="ACHOTW_005020"
     CDS             827..1117
                     /locus_tag="ACHOTW_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP9846928.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            1188..1466
                     /locus_tag="ACHOTW_005021"
     CDS             1188..1466
                     /locus_tag="ACHOTW_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846929.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            1599..>1807
                     /locus_tag="ACHOTW_005022"
     CDS             1599..>1807
                     /locus_tag="ACHOTW_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846930.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLD"
BASE COUNT          509 a          399 c          513 g          386 t
ORIGIN      
        1 agcccggcca aactccgcga taagtggacc caactcgaaa tcaaccgtaa caagcaacag
       61 gcaggcgtga cagccagcaa accaaaactc gacctgacaa acacagactg gatttacggg
      121 gtggatctat gaaaaacatc gccgcacaga tggttaactt tgaccgtgag cagatgcgtc
      181 ggatcgccaa caacatgccg gaacagtacg acgaaaagcc gcaggtacag caggtagcgc
      241 agatcatcaa cggtgtgttc agccagttac tggcaacttt cccggcgagc ctggctaacc
      301 gtgaccagaa cgaagtgaac gaaatccgcc gccagtgggt tctggctttc cgggaaaacg
      361 ggatcacctc gatggaacag gttaacgcag gaatgcgcgt agcccgtcgg cagaatcgac
      421 catttcttcc atcacccggg cagtttgttg catggtgccg ggaagaagca tccgttatcg
      481 ccggactgcc aaacgtcagc gagctggttg atatggttta cgagtattgc cggaagcgag
      541 gcctgtatcc ggatgcagag tcttatccgt ggaaatcgaa cgcgcactac tggctggtta
      601 ccaacctgta ccagaacatg cgggccaatg cgctgactga cgcggaatta cgacgcaagg
      661 ctgccgatga actgacctgt atgacagcgc gaattaaccg tggtgaggcg atacctgaac
      721 cagtaaaaca acttcctgtc atgggcggca gacctctaaa tcgtgttcag gcgctggcga
      781 agatcgcaga aattaaagct aagttcggac tgaaaggagc aagtgtatga cgggcaaaga
      841 ggcaattatt cattacctgg ggacgcataa tagcttctgt gcgccggacg ttgccgcgct
      901 aacaggcgca acagtaacca gcataaatca ggccgcagct aaaatggcac gggcaggtct
      961 tctggttatc gaaggtaagg tctggcgaac ggtgtattac cggtttgcta ccagggaaga
     1021 acgggaagga aaggtgagca cgaacctgat ttttaaggag tgtcgccaga gtgcagcgat
     1081 gaaacgggta ttggcggtat atggagttaa aagatgacca tctacatcac tgagctaata
     1141 acaggcctgc tggtaatcgc aggccttttt atttggggga gagggaagtg aacgatagct
     1201 accgacagtt tgaaaactgg tggtcaaaag acaaaagcca gttcacggga gacgatgaat
     1261 taaaagagtt tgcctgggtg atatggcagg catcgcgctc tgctattgaa ctggatatcg
     1321 actggcccga atcgaatgac gacctttgga aagatggtga agaaggtgct tatgcgatgg
     1381 gttatgagga tgggcgtgac aaaacggtaa ttgcagtaat gaaagccatc agggccgcag
     1441 gaatcaaaga aaagaatttc gattaagcaa atatcacttc aataaatcgc ttttaaggca
     1501 tcacaatcgc tctgtggtga ggtaagcacg tgcaaggtat gccgataagc agcgagaatg
     1561 aaaaatgcgt cagaatgcgt ttgaggaggt tttaagaaat gagtacgata gctgagcttg
     1621 tcagggctaa ttttcgtgaa gagttggtgc gttggtatcg gtatcgttca tcgtccagtt
     1681 tgccgcttga tgagttgtat gagcattcac ctgccgcacg acgctatccg cgtgaccgtg
     1741 ttcttcgacg gttgttcaaa ctcaacaatg agtttcagcg caacagaatt atccggagtc
     1801 tggattt
//
LOCUS       DAZKIF010000321         1795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZKIF010000321 DAZKIF010000000
VERSION     DAZKIF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.324"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..350
                     /locus_tag="ACHOTW_005023"
     CDS             <1..350
                     /locus_tag="ACHOTW_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846931.1"
                     /translation="NNPIIKKFISAEDYWIFFLISLIKFPETAEELLNNALVTLPADA
                     NYKDKTLLLKAIYSGCTNLPFSLFINNEQLLEIRECCKQAIKVTFAAELFDTQNCNKK
                     QNKKPWKKVMFNV"
     gene            343..>1795
                     /locus_tag="ACHOTW_005024"
     CDS             343..>1795
                     /locus_tag="ACHOTW_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9846932.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIVVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYN"
BASE COUNT          532 a          281 c          376 g          606 t
ORIGIN      
        1 tgaataaccc aataattaaa aaatttatca gcgcagaaga ttactggata ttttttctga
       61 tatcgttaat aaaatttcct gagactgcag aggaactcct gaacaatgca ctggtgaccc
      121 ttcccgcaga tgctaattat aaagacaaaa cgttactcct gaaggcgatt tacagtggat
      181 gtacaaatct tccattttca ctttttatta ataatgaaca attattagag attagggagt
      241 gttgtaaaca ggcaataaaa gtaacatttg cagcagagct ctttgataca caaaactgta
      301 acaaaaaaca aaataaaaaa ccatggaaga aggtgatgtt caatgtttaa caaagttttg
      361 gtagggttaa ggagtcatcc agaattaata attcttggat taatggtgat gattatcgcc
      421 atgctcataa ttccattgcc cacctattta atcgactttt tgattgggct taacttaaca
      481 ttggcaatac ttgtattttt aggatcattc tatgttgata ggatcttaag cttttcttca
      541 tttccatcaa tcctccttat cacaacgtta tttcgtttgg cgctggcgat cagtaccagt
      601 cgacttatac ttcttgaggc ggatgctgga gaaattataa cgagttttgg tgagttcgtt
      661 attggagata gcctggtagt tggttttgtt attttctcca ttgttactat tgttcagttt
      721 atcgtgatta ctaaaggttc ggaacgtgtg gctgaagttg ctgcccgctt ttcgcttgac
      781 ggtatgccag gtaaacaaat gagcattgac gccgatctac gcgccgggat cattgatgca
      841 gatttagcaa aagaacgtcg tagtgtatta gaacgagaga gccagttgta tggttccttt
      901 gatggtgcga tgaaatttat caaaggtgat gctattgcaa acattattat tatctttgtt
      961 aatattatcg gtgggctatc agtaggagta ggccaaaatg gaatggattt ctcaacagcg
     1021 ttgactgtct acacaattct tactgtcggt gatggtcttg tttctcaaat tccagcttta
     1081 ttaattgcta ttagcgcagg atttatcgtt acgcgcgtaa atggcgatag tgataatatg
     1141 gggcaaaata taatgtccca gttattaagt aattcttttg tcattgttgt gacatgtgtc
     1201 ctcgcgctaa gtattgggtt gcttccaggt tttccccttc tagtgttttt atgtttggca
     1261 gtaatcttag gaatttactt ttactttaaa ttcaaaaaga aagggactga agaaacggtt
     1321 gttgagggag atattaccgt tgggttggaa ccctataatc aagatgatga tatctcatta
     1381 ggtatcatta ataaacttga tcaggttatt accgaaacgg tacccttggt cttgattatg
     1441 aatagtgtac aggctaaaaa atacactgaa atcaatcttg ctgataggat tcgtagtcag
     1501 ttttttattg agtatggtat ccgcattccc ggtattgtca ttcgtgaggg ggaaggtctt
     1561 aatgatgaag atgtcatatt aatgctaaat gaagtcagag cttcacaatt taaaatttat
     1621 catgatcttg tcttgttggt tgaatattca gatgaagtgg tttcgacttt gatcaagaaa
     1681 cctgtcattg taaatagtaa tggtgaacaa tattattggg ttacgaaaag tgatgctcaa
     1741 aaattaacaa aaattggctg ttatactcgc acggccatgg atgaaatgta taatc
//
LOCUS       DAZKIF010000322         1760 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZKIF010000322 DAZKIF010000000
VERSION     DAZKIF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.325"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            410..694
                     /locus_tag="ACHOTW_005025"
     CDS             410..694
                     /locus_tag="ACHOTW_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFP9846933.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            694..969
                     /locus_tag="ACHOTW_005026"
     CDS             694..969
                     /locus_tag="ACHOTW_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9846934.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            1064..1270
                     /locus_tag="ACHOTW_005027"
     CDS             1064..1270
                     /locus_tag="ACHOTW_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846935.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLDR"
     gene            complement(1370..1585)
                     /locus_tag="ACHOTW_005028"
                     /pseudo
     CDS             complement(1370..1585)
                     /locus_tag="ACHOTW_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1629..>1760)
                     /locus_tag="ACHOTW_005029"
     CDS             complement(1629..>1760)
                     /locus_tag="ACHOTW_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9846936.1"
                     /translation="NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          470 a          420 c          434 g          436 t
ORIGIN      
        1 ccggcccaaa gggccggaac agagtcgctt ttaattatga atgttgtaac taagcatcat
       61 catcgctgtc agtcttctgg ctgaacgttc tcagtacacg ctcgtaagcg gccctgaggg
      121 ctcgctaacg cggagatacg ccccgacttc gggtaaaccc tcgtcgggac cactccgacc
      181 gcgcacataa actctctcat ggctgaaagc gggtatggtc tggcagggct gggggggggg
      241 taaggtgaaa tccatcaatc agtaccggct tacgccgggc ttcggcggtt ttacacctgt
      301 gctacatgta acctccgtat accgccttac atgcgctgac gcgcattatt tgccttgttc
      361 cgtctgaaag taatcactat gattaaatat gattaacgac taatcggata tgcaaatgaa
      421 aaacaatacc gcacaagcaa caaaagtcat taccgcgcat gtgccattac ctatggctga
      481 taaagtcgac cagatggccg ccagactgga acgctcccgg ggttgggtta tcaaacaggc
      541 gctttctgca tggcttgccc aggaggagga gcgtaatcgc ctgacgctgg aagccctgga
      601 cgatgtgaca tccggacagg ttattgacca tcaggctgta caggcctggg cggacagcct
      661 cagtactgac aatccgttac cggtgccacg ctgatggaac tgaagtggag cagtaaagcg
      721 ctttctgatt tggcgcggtt atatgatttt ctggtgctag ccagtaaacc tgctgccgcc
      781 agaacggtac agtccctgac acaggcaccg gtcattctgt taactcatcc acgtatgggg
      841 gaacagttat ttcagtttga acccagggag gtcagacgga tttttgctgg cgagtacgaa
      901 atccgttacg aacttaatgg ccagactatt tatgtattgc gcctgtggca cacacgagaa
      961 aacaggtaga ttgtgtgtgg ggacaactta acatagcctg cagatattaa ttctggaaca
     1021 ttattatcag gccagatatt taaataatct tgtaggagat aaaatgaatt acgatgaaat
     1081 aacgaaaatt accgctgaac gtattagcga ctacatgact gaagcagtta atacagactc
     1141 tatagccgtt gcggaaatgt ttcataacgc agcatgggga gtgcgaacat tgtggtttga
     1201 actcgtcaca aaaatagata tagatataca taaaaagaac agatatgcga gttatgatct
     1261 tgatcgatag gaatttaaat cccaaaaaga ttaaaaaaac acccaaaaac ggatgtttat
     1321 tcaacactgc ctgcgtgcca tgagtgcacc gccgtcaatt aaatggatct atttcatcct
     1381 gaccagcata gtgctccaga cggctcatcg gcaccggcgg ttttttcagg taagagccga
     1441 agtacaccgc cacatgtgtg gcgttatcca tcacccggga tacgttgaca ttccagcccc
     1501 gccggtaatg actgtccagg aagcgattcc tgtcccgttt actgcttcct tctgctgcca
     1561 gcgcatccgg tatcaccagg tccggtgagg tgtactggca atagcggaca ctaccatttg
     1621 ttcttttttt aagcagccat ctgatgatat ttttccctga aggctgccgg ggagatattc
     1681 cccagacgag agtgacgacg ctgacgattg tagaaaatct caatgtattc ccgtattact
     1741 gagatggctt catcccggtt
//
LOCUS       DAZKIF010000323         1743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZKIF010000323 DAZKIF010000000
VERSION     DAZKIF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.326"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(69..734)
                     /gene="perB"
                     /locus_tag="ACHOTW_005030"
     CDS             complement(69..734)
                     /gene="perB"
                     /locus_tag="ACHOTW_005030"
                     /EC_number="2.3.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine N-acetyltransferase"
                     /protein_id="HFP9846937.1"
                     /translation="MNLYGIFGAGSYGRETIPILNQQIKQECGSDYALVFVDDVLAGK
                     KVNGFEVLSTNCFLKAPYLKKYFNVAIANDKIRQRVSESILLHGVEPITIKHPNSVVY
                     DHTMIGSGAIISPFVTISTNTHIGRFFHANIYSYVAHDCQIGDYVTFAPGAKCNGYVV
                     IEDNAYIGSGAVIKQGVPNRPLIIGAGAIIGMGAVVTKSVPAGITVCGNPAREMKRSP
                     TSI"
     gene            complement(778..1709)
                     /locus_tag="ACHOTW_005031"
                     /pseudo
     CDS             complement(778..1709)
                     /locus_tag="ACHOTW_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          526 a          376 c          274 g          567 t
ORIGIN      
        1 tagtaaatta gcgaaattat atatatttta aacattagtc ccatttggaa cgtgttttcg
       61 cattcccatt aaatagatgt tggcgatctt ttcatttctc ttgctggatt tccgcacaca
      121 gttataccgg caggaacact tttagtgaca acagccccca tacctataat ggctcccgcg
      181 ccaataataa gtgggcgatt aggaacaccc tgcttaatta ctgcacccga gcctatatat
      241 gcattgtctt caataacaac atatccatta catttagccc caggagcaaa tgtaacatag
      301 tctcctattt gacaatcatg tgcaacgtat gagtatatgt ttgcatgaaa aaacctccct
      361 atatgagtat tagtagatat tgtaacaaag ggagaaataa tagcgccact acctatcata
      421 gtatgatcat aaacaacgct atttggatgt tttatagtta ttggttcaac cccatgtaat
      481 aatattgact cagacactct ctgtcgtatc ttatcattag caatagcaac attaaaatac
      541 ttttttaaat aaggggcttt tagaaagcag ttggttgaaa gcacttcaaa accattaact
      601 ttctttcctg ccaaaacatc atccacaaaa accagagcat agtcagaacc acattcttgc
      661 tttatttgtt gatttagaat gggtattgtt tctctaccat aacttccagc accaaaaata
      721 ccatacaaat tcattttccc atctccaaag aaatatctta tcaaaaccaa tgcaagatta
      781 tgaaagcccg cacgctgcaa tgcctgacgc taggaagttt ctgtccatga ctgcttttcg
      841 catcttacat gataatcctg ccttgaatac attatcattg tcagatatta atagcgatgt
      901 gccgcattct agaaaatcat tcgaatgcaa ctcgccttct tatattatag tcgatttcat
      961 tcattaccac atcaggctac tgtgcaactt attctccatg cgccagtgat ttcgatttac
     1021 tgtggcgaac ttctctgcgg ttaaagcagc agatctaata taatatttga tcgtcatttc
     1081 ggattctttc ttttgctctg ctattattga gagaaagtgg actgccattc ataaattctg
     1141 cagccctttc cattcaaacg tgaaatcaat aagctcatca ggagcatctc aaacaatatg
     1201 aagacggaca tcgtctctgc cgtgcctctt ttcactaatt gcgtaactgt catgttttgg
     1261 attatttaat tctttcagcg taaatatctc ctcaaagact ctattaagcc gactcttatt
     1321 tccttttaca gcgaataaat aatcacatct ctgtttttat atcttctctg caatatcttt
     1381 ctggcaagcc atcgcatcag ttatgattat ttttccttta atacccatca ttttagaaag
     1441 ttcatgaata actgtgaacg cattaggttt ctcatccgtc ttgatctatc cgaggaccag
     1501 actgtgcatt gctgaaacgc actaatgaca tgaaccactc ctctgcgacg actcttgtca
     1561 taagtgtgcc gaagtgtttt accatcaatt gcgatgacgt cgttatcatc tggaaaatgg
     1621 cagacatgta ttcagttaac aaagcactcg tgaaatattg caggactgat gcaggataca
     1681 actttggcta taatatcgta aacagggata ataaaaatct attgtctcaa ttattttaag
     1741 ttc
//
LOCUS       DAZKIF010000324         1734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZKIF010000324 DAZKIF010000000
VERSION     DAZKIF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.327"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..158
                     /locus_tag="ACHOTW_005032"
     CDS             <1..158
                     /locus_tag="ACHOTW_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846938.1"
                     /translation="FSEQIFKITGDENAAKNELEPWTPEGADPNYCWREVDPVDAANE
                     AMSYHND"
     gene            191..408
                     /locus_tag="ACHOTW_005033"
                     /pseudo
     CDS             191..408
                     /locus_tag="ACHOTW_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
     gene            410..673
                     /locus_tag="ACHOTW_005034"
     CDS             410..673
                     /locus_tag="ACHOTW_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846939.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            684..845
                     /locus_tag="ACHOTW_005035"
     CDS             684..845
                     /locus_tag="ACHOTW_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846940.1"
                     /translation="MTTITKERIELFIKNPVENGLTRGEQMELARIALASLEAEPVGD
                     FYEYKPDDW"
     gene            903..1007
                     /locus_tag="ACHOTW_005036"
                     /pseudo
     CDS             903..1007
                     /locus_tag="ACHOTW_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1601..>1734
                     /locus_tag="ACHOTW_005037"
     CDS             1601..>1734
                     /locus_tag="ACHOTW_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP9846941.1"
                     /translation="MNVIDLFSGVGGLSLGAARAGFDVKMAVEIDQHAINTHAINFPR
                     "
BASE COUNT          531 a          308 c          434 g          461 t
ORIGIN      
        1 aattctctga gcagattttt aaaattactg gcgatgagaa tgcggcaaaa aatgaattag
       61 aaccgtggac gcctgaagga gccgacccaa attattgctg gagggaggtt gatccagttg
      121 atgctgcaaa tgaagctatg agttatcaca acgattaatg tcaggaggcc gcccgaaagg
      181 gcggtaatga atggtcacat tatttagaaa aaatatccgc gaaagagtag aacaacagaa
      241 tttctgtttc tcattctgtt tatcgtgttg atgataccga tatccccgtt aatcctagtc
      301 tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt ggtatgggcg
      361 tcattcaaca cactgcacaa taaaattaat ccgtataagg aaaactgata tggcaacttt
      421 gacaaaaaaa gaacgggcat ggttgaacga attacaggaa gttcttgatc gctgtccatc
      481 accgaaaaaa attggctttt acaccattgg cgataaaagc atttacctgt atgacctacg
      541 ccgcatggat gaaatcatgg aggctcttga taatcgttcg tcgatggatt ggtgtgttgc
      601 tgttcatgat atgaatgcag ggtttgatga aaagattttg ttcccctcat cagttgaaag
      661 cactgcgggt taaggagtaa cacatgacca ctattaccaa agaacgtatt gaattgttca
      721 ttaaaaatcc ggttgaaaac gggcttaccc gtggtgaaca aatggaactg gcacggattg
      781 cgctggcatc gctggaagca gagccagttg gtgattttta tgaatacaaa ccggatgact
      841 ggtagcagcg ttcggctgga gataaagcgc caaaatggac gccaccctat gccgtttttc
      901 cagcgcccgt agtgccggaa gaaatggatt tgcttacatg ccatctcgat ggtgtaactg
      961 aaacatatgc tgagggatgg aacgcttgcc gcgctgccct gcttcagggc aaaggggagc
     1021 ccgggaaaca agttcgcgaa ttgacaatgc tggttaaaca attggttagt caactgaaga
     1081 aagcgaaacc gggctgcaaa ttgccggata aagtgatgga ctacctggag cgaagcggac
     1141 ttataagcgt ggaggatgtt tcacgatgac ctggcctgaa gcattcacaa cggtaggaat
     1201 tgcgatggcg gtggcgctgg ttgtgtattc gatttgccgc tggggataaa aacgatttgc
     1261 gggaaaagga tagttaagta gaattgctgc gggtgcttga ggctatctgc cttgggcatg
     1321 aacaccaaag gcagatagag aaaaacccac ccgactataa atcaaagtgt attaccccca
     1381 tttgttggac gatgaaatgg gtttagttaa ctatgtcggt atcgacatga ctaaaacaac
     1441 cttaaattgc tcgattaata gacaagtagt tttgaaagat taaatttatg cgatccggta
     1501 ttttcaaggg atagccttta tgaggttatc ttatggggtt aaaacatcaa gaacagttgt
     1561 gttataatac atagaaacta ataagagacg ttgcaactat atgaatgtaa tagatttgtt
     1621 ttctggcgtt ggaggtctaa gtcttggtgc tgcacgtgct ggatttgatg ttaaaatggc
     1681 agttgaaatt gatcaacatg ctattaatac tcacgcaatt aattttccaa gaag
//
LOCUS       DAZKIF010000325         1723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZKIF010000325 DAZKIF010000000
VERSION     DAZKIF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.328"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..910
                     /locus_tag="ACHOTW_005038"
     CDS             <1..910
                     /locus_tag="ACHOTW_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HFP9846942.1"
                     /translation="NRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYQYWRKR
                     RDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSS
                     CQPGKHQYKNARQEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLF
                     ARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYR
                     IKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPH
                     HYNGGLTPEESENRYHFYCKTVASIT"
     gene            919..1071
                     /locus_tag="ACHOTW_005039"
                     /pseudo
     CDS             919..1071
                     /locus_tag="ACHOTW_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            1161..1535
                     /locus_tag="ACHOTW_005040"
     CDS             1161..1535
                     /locus_tag="ACHOTW_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9846943.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            1532..>1723
                     /locus_tag="ACHOTW_005041"
     CDS             1532..>1723
                     /locus_tag="ACHOTW_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9846944.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFT"
BASE COUNT          435 a          455 c          453 g          380 t
ORIGIN      
        1 caaccgcgct cttgatgtcc gactcgctga acggttcacg atagttgcca gactgagtga
       61 cagccactca gttgtcagcc tctgttctgc tctggaaata caccgcagca gttaccagta
      121 ctggcgaaaa cgacgcgata cggttaatcc ggcgcgagtc aggttgtgca gcgaaatacg
      181 ccgggcgtgg aaccaaagta ggggctctgc gggggcgcgc acgctggctg aaatgctgac
      241 ccaaaacggc gtcccgatga gccgttaccg tgccgggcgt ctgatgaaat atctgaacct
      301 gagcagttgt cagcccggaa aacatcagta caaaaatgct cgtcaggaac atacctgcct
      361 gccgaatctg cttgagcgcc agttcgctgt gcccgagcca gatagggtat ggtgcggaga
      421 tattacgtat atctgggcag gaaatcgctg gtgctatctg gcggtcgtca tggatctttt
      481 tgcccgcagg gttatcggct ggagcctgtc agcgaatgcc gatactgccc tgataagcag
      541 tgccctgcgg atggcgtatg aggtgcgtgg tcagccgcgg gacgtcatgt tccatagcga
      601 ccagggaagt caatatacag gactgaaata tcaacaactt ctctggcgtt acaggataaa
      661 gcaaagtgtc agccgacggg gaaactgctg ggataacagt ccaatggaac gcttcttccg
      721 cagtctgaaa acagaatggg tgccaaccga tggctacaca ggcaaagacg tggcccggca
      781 gcaaatcagc agttatattc tgaattatta caatagcgtc aggcctcacc attataacgg
      841 agggctgacg ccggaagagt cagagaacag atatcatttt tactgtaaaa ccgtggccag
      901 tattacttga ccactacact ggaactcatg ctcaccagcg gtgagctgaa tccccgccat
      961 cagcataccg tcacgctgta tgcaaaaggg ctgacctgca aagccgatac cctcggcagt
     1021 cgaggttacg tttatatggc tgtttatccg acgcccgaaa cgaaaaagta aatcatcaga
     1081 ataaccttct gctccggcct ggtgttttca ccacgccact tccccatttc tcatctctgc
     1141 atatcaggaa aaactttagt atgaaaacat tacccgatac acacgtacgg gaggcatcgc
     1201 gctgcccgtc tcccatcacc atctggcaga cactgcttgg ccgtctgctg gaccagcact
     1261 acggcctcac gctgaatgac acaccgttcg ctgatgaacg tgtgattgag cagcatattg
     1321 aggcaggtat ttcactgtgt gatgcggtga actttctcgt ggaaaaatac gtgctggtgc
     1381 gtaccgacca gccgggattc agcgcctgta cccgctctca gttaataaac agcatcgata
     1441 tcctccgggc tcgcagggcg accggcctga tgacccgcga caattacaga acggtaaata
     1501 acattaccct gggtaagcat ccggaggcga aatgatgaaa ctggccctga cactggaagc
     1561 cgacagcgtt aacgtacagg cactgaatat ggggcgaatt gtcgttgaca ttgatggtgt
     1621 taatctcgct gaactgatta acatggtctg cgataacggc tactcccttc gtgttgttga
     1681 tgaatctgac cggacctcag cagactgcac gccaccattt act
//
LOCUS       DAZKIF010000326         1702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZKIF010000326 DAZKIF010000000
VERSION     DAZKIF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.329"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(220..726)
                     /gene="espH"
                     /locus_tag="ACHOTW_005042"
     CDS             complement(220..726)
                     /gene="espH"
                     /locus_tag="ACHOTW_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HFP9846945.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
     gene            complement(757..1647)
                     /gene="escQ"
                     /locus_tag="ACHOTW_005043"
     CDS             complement(757..1647)
                     /gene="escQ"
                     /locus_tag="ACHOTW_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFP9846946.1"
                     /translation="MKINDFYLPLLPVIGTGRLYITSKGHACHAYFREVSGHGIRFTL
                     TYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTALPEGDVSVDD
                     VQFTMLNKDIYPVIENNNGENRLNVIILETTVQSLQYLINDNWQLVPHSNALFFDGYI
                     VPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVINTPLATVNLSDNNLSVTDVLAAD
                     LLSALSNETVMNRIYCAIGTVHIDIHMLRNVKKDDIINSDGYHLFGGCQLIRNNTTIA
                     YGSIVKINEDFYFTVSIVCD"
     gene            complement(1637..>1702)
                     /locus_tag="ACHOTW_005044"
     CDS             complement(1637..>1702)
                     /locus_tag="ACHOTW_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFP9846947.1"
                     /translation="FDKLKEIFPDEAIEFTIEYEN"
BASE COUNT          533 a          365 c          286 g          518 t
ORIGIN      
        1 acctacagat tttttacctt gataatttct gcgctggatg ggatcatcca ttccgtttaa
       61 catactcttc atcacaggga cctctgattt ctggccgcta acgtctctga agattcagcc
      121 tgtattatct aaaagcaatg taatatcagt taaaaagcgg aaaaactcca gaattataac
      181 tattctggag ttaagtcagg aagaaaactc aaatgctaat cataatacgc tataagagga
      241 agctcttgtt gctatagccc tattatcaag acgttcggta gactgtacac gacgtaacaa
      301 agaagataat cctctttcag ctaagatccc ctccagggga gtacgaggaa accaggttga
      361 agaaagtgga gttatatgtc tctcatttaa ttttctttcc aaagccttag tcacaccgcc
      421 agaaaacacg ctaaatagcg ttgagaaaca tccattttcc ggtgttattc tttgtacttt
      481 gtacacataa tcatcttttg ggccatcttt aataaagtga acagcatagt ctttgcctct
      541 aaaagagaca atggtggaat cccctatttc tggatatacc cgatgatggc gttgttgcat
      601 tcgggcttta agctgaattt catctacgat atcttcattg ggtatatgaa gccttctgtt
      661 gaacctgttc catgacgcat gttcacttgt cagaaatgtc ttgaataccg ctcctgataa
      721 cgacatgata catctcccta tataacgtat tcctgattaa tcacatacta tgctaacagt
      781 aaaataaaaa tcttcgttga tttttacaat actcccgtat gctatcgtcg tattattgcg
      841 tattaactga caaccaccaa aaagatggta tccatctgaa ttaataatgt catctttttt
      901 aacattacgc aacatgtgta tatcaatatg gactgtaccg atcgcacaat aaatgcgatt
      961 catgacagtc tcattgctaa gagcagataa cagatccgca gccagaacat ccgtgacaga
     1021 taaattatta tcactcagat ttactgttgc taaaggggta ttaataacaa tccagcacct
     1081 tctctcttga ctatcatcaa catgatagag tctcagacta tcaccaactc tcaactctgt
     1141 aactggataa tcagtccacc caggtacgat atagccatca aaaaaaagag cattagaatg
     1201 tggaactaat tgccaattat cattaatcag atattgtaaa ctttgtaccg ttgtttcgag
     1261 tataattaca ttaagtctgt tttctccatt attattttct attaccggat aaatatcttt
     1321 atttagcatc gtaaattgaa catcatcaac tgacacatca ccttccggta aggcagtctg
     1381 cattacccat aagatcatga gttttatcgt atcttccggt atcagccggc tttcgctata
     1441 cgggaataac tcttggcacc actgaatgaa ctgttcttct gatatccaga aacgcccctc
     1501 atagcccgag tatgtcaacg tgaaacgaat accatgaccg ctgacctcac gaaaatatgc
     1561 atggcaagca tgacctttgg acgtaatata gaggcgaccg gttccgataa ctggcaacaa
     1621 cggcaaataa aaatcgttaa ttttcatatt caattgtgaa ctcaatggct tcatcaggaa
     1681 agatttcttt taacttatcg aa
//
LOCUS       DAZKIF010000327         1690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZKIF010000327 DAZKIF010000000
VERSION     DAZKIF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.330"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1690
                     /locus_tag="ACHOTW_005045"
     CDS             <1..>1690
                     /locus_tag="ACHOTW_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9846948.1"
                     /translation="GAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
BASE COUNT          522 a          396 c          438 g          334 t
ORIGIN      
        1 ccggggctgt cactgttgcc attcgccgcg aatacaaaca gacatggaaa acactcgaca
       61 atgaactggc ctgcgccctc tggcccggtg atgtggatgc aggaaacatt gacggtacca
      121 tccatcgctg ggcgacaaat gaggttatcg acaaggatcg cgaagactgg aagcgtatct
      181 cagcatcaat gcgcaaacag cccgaagcac ttggctatga ccgtcagact atttttggcc
      241 ttgttcgcga acgtccgatc gatattcaca aagatcccgt tgcactgaac aaatatatca
      301 gtgaatacct gacgacaaag ggcgtgtttg aacatgaaga aacagaccag agctctactg
      361 atgctctcca gccgtcagca gcacaaactg ctccagtgga gacggcagaa tccgatactc
      421 aaaaaaatga aatcctggtg gaagctgaac catctgtaga gcgtgaagga ccattttatt
      481 tcgtctttac cgataagggc ggggaaaaat acggcagggc aaacaaactt tctggtctgg
      541 acaaggcgct ggctgccggc ggtaccgaaa tctcaaaaga agaatatttt gcccgaaaaa
      601 atggcacata cacgggctta ccgcaaaatg tggataccgc tgaagattcc gaacaatcag
      661 agccggtaaa agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc
      721 agcctgacgc cgataaattg cttgctgcat cacgtggtga atttgttgaa gggattagcg
      781 acccgaatga tccgaaatgg gtgaaaggga tccagagtcg cgacgctgag gaccagaatc
      841 agcccaacgt gaaacaaaat gagccagaag cggaacaaaa cagcccggat acgcaacaaa
      901 acgggccaga agaacaacaa ccagaaccag cagtgcaaca ggaactggaa aaagtttgca
      961 ccgcatgcgg tcagaccggt ggcggcaact gtcctgactg tggtgcggtg atggggaacg
     1021 caacctacct ggaaacattc gatgaagaga atcaggctga agctcagaaa aatgatccgg
     1081 aggaaatgga aggcactgaa catctgcaca aggagaacac tggcagcgat cagtatcacg
     1141 ccagcgataa taaaactggc gaaacagcaa atcccttaat taaagtgaac ggtcatcatg
     1201 aaatctcatc caccagcagg ttgtggcacc atctgatgat tgaccttgaa acaatgggaa
     1261 aaaatcctga tgcgccaata aactctatag ccggtaagtt ttttgatccg gcaaccggag
     1321 agatggggcc agaattcagc aaaactatcg atctggaaac cgcaggtggg gtcatcgatc
     1381 gggacaccat taagtggtgg ctgaaacagt cacgcgaagc acaatcctcc attctgaccg
     1441 atgaaatcac gttggatgat gcactgctgc aattccggga atttatcgac gaaaactccg
     1501 gtgaattttt tgttcaggtc tggggtaacg gtgcaacttt cgacaacgtg attttacgcc
     1561 gttcatatga acggcagggg atcccctgcc cgtggcgtta caccaatgat cgcgatgtaa
     1621 gaacgatggt tgctctggga ctggtgatgg atttcgacgc aagaacgact attccattcg
     1681 aaggtgaacg
//
LOCUS       DAZKIF010000328         1661 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZKIF010000328 DAZKIF010000000
VERSION     DAZKIF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.331"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..894)
                     /locus_tag="ACHOTW_005046"
     CDS             complement(<1..894)
                     /locus_tag="ACHOTW_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HFP9846949.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGDGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVA"
     gene            complement(891..1640)
                     /locus_tag="ACHOTW_005047"
     CDS             complement(891..1640)
                     /locus_tag="ACHOTW_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9846950.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIHTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          488 a          362 c          272 g          539 t
ORIGIN      
        1 agctactaac ttctcgataa aatcaacctg agatactgtt cctttaacaa gtaaactgtt
       61 ggtatcagga tatgcaataa tttgtatatc gtcagtctct ggcgctcctg ctgtcgatat
      121 tattttctct acttgtaggt catcatcttc ttcagcccca ggaaaaggag gcatacgtga
      181 tacgtttttt agttgttgac gacttgaaat ttcagaaagt acgttcaccc ctgtgctttg
      241 cttaatgtta ttatttaaca acgtcgatag tacttttgcc atgccaggaa taactatctt
      301 ttcgccacgt aactcataag tccggtcatt aacgaatgtg ttgaccagat gaattattcc
      361 tactttattt cgaccgatct caatgccatc gctgttttgc tccattaatt tcgcagcatt
      421 aacgaccagg tcaacataaa ccggtggccc cgatacatag aaagtaccat tgccgtcccc
      481 tttaatttca taacgagagt tatacagacc cgattttttt aagaaattat taaactcatt
      541 cgtcgatacc tttctcagat taatcaatgc attacgcatc tctgatgagt catagatata
      601 aatagctttg ccatcatcat accaaataag ccccatctgt gccgacaggc gttcaatcaa
      661 ggcatagggt tctgttaaat caaaatttcc gctgatctgt ttcttcatgg caagcttact
      721 aactattgta ggcttacccg catatgatga aaccgtttca aaaaaactac gtagattttc
      781 tttatttgct acgtatccat tcgctgctgc ggcatatgca ccaggagtca ataatccatt
      841 aaaaatgcat aagactgaaa ttaatataat agtaatgcgt aattttattt tcataacttt
      901 ttacatagtt ctctcggtgt ttttcctaac cggcttttta cttcggctga aaagtgtgat
      961 gaggacgagt aaccatattt atgcgcaata gttgtcatat cactgtttcc ttcaatgatt
     1021 tccaacacgg ctctggcaac tctccagcca cataattccg ttttaacttt accacccaat
     1081 gcataactac ataaacggcg aaaatgtgaa tacgatacgc cgtaaagctc accaagatca
     1141 tagagtgaag tatgtatgag cgattgcgat aataaataac gaaccagttt atagcattct
     1201 gtttttctta gatgctgaaa caggatttca ttaatattat cgctgctcag ataagtggct
     1261 aaaaaccagg cttcacttgc aactttactg aatagcacaa cttctgatgc aaagggcaag
     1321 gcatcagcaa acaattcatg gtgttccctt tccacactcc ccgttgattc atctataaaa
     1381 gctaataatt tactcaggat atcaatgcga agtggatgat aaattacttt ccctgcagtt
     1441 acctcaattt ggcttcggtc aatgagcagt aatatatctt ccactaccat taatttttga
     1501 tggttattac caatcgtaat aacaagggtt acatccttgt tttcatttcc ccgtaagaac
     1561 cacatttcag ggtccagtag ggttaacttc tgtccttgaa gcaaaacatt cattggaagt
     1621 aacagccctt cttcaatcat gtttcatctc cttattccat a
//
LOCUS       DAZKIF010000329         1644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZKIF010000329 DAZKIF010000000
VERSION     DAZKIF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.332"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1396
                     /locus_tag="ACHOTW_005048"
     CDS             <1..1396
                     /locus_tag="ACHOTW_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFP9846951.1"
                     /translation="LYLKQHLRRNVSTRSSFSAETYRDEFDRFIKRFPIIGSSTHSII
                     NSIGKGALLDYVIIDEASQQDIVPGILGLGCARNVIVVGDRKQLPHVPVLLPNSPSPP
                     AEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCHPKIIQFCNKQFYDNALIPLTVDS
                     GEASLSLVITAKGNHTRNFSNLRELESLEGHYWDEESSRGYIAPYNAQVNLAEKVLPA
                     DFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRNIAFVDNPELVNVAVSRARNKFTL
                     VTGNDVFERHAGHIAALIRYIKYYADDGEIFESPVISAFDLLYSEYDKSLERLNSRLN
                     SNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIALNQLVLLERGDFTHREQLFMRNR
                     ASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERDELKNSILKKCGLPLLRLRTIDSD
                     IEGKLGAFLSGLTG"
BASE COUNT          464 a          304 c          392 g          484 t
ORIGIN      
        1 gctttattta aagcaacatc tgcgaagaaa tgtttcaaca agaagttcat tctcggcaga
       61 gacatacagg gatgaatttg accgttttat aaagcgtttt ccgattattg gtagcagtac
      121 gcattcgatt attaactcta tcggcaaggg ggcccttctt gattatgtta ttattgatga
      181 agcctcgcag caggatattg ttccgggcat tctgggactg ggatgtgccc gtaatgtaat
      241 cgttgtaggg gatcgtaagc aacttccaca tgttcctgta ttactcccga acagcccatc
      301 tccaccggca gaatactata attgtgagaa atatagtcta cttgattcgg tttgtatgtt
      361 gttcaggaac atggtgccag taacccttct taaagagcat tatcgttgtc atcctaaaat
      421 cattcaattt tgtaataagc agttttatga taatgctctg ataccgttaa cggttgattc
      481 cggtgaagcc tcattatctc tggtcatcac ggcaaaagga aaccataccc gtaatttttc
      541 aaatctcagg gagcttgaat ctctggaagg tcattactgg gatgaagaaa gtagcagggg
      601 atacattgcc ccatataatg cacaggttaa tctggctgaa aaagtgctgc ctgctgattt
      661 tgtgaaatcg acggtgcata aatttcaggg acgggagtgc gatgaaattg tgttttccac
      721 tgtgctggat aagaagcgca gcagccagca cagtcggaac atcgcgtttg ttgataatcc
      781 tgaactggtg aatgtcgctg tttcaagggc ccgaaataaa tttacgctgg tgactggtaa
      841 tgatgtattt gaacgccacg ctggacatat tgcggcgctg attcgttaca tcaaatacta
      901 cgctgatgac ggagagattt tcgagagccc ggtcatatca gcatttgatt tgctgtacag
      961 tgagtatgac aaatcactgg aacgactgaa tagtagattg aattcaaatg attcacactt
     1021 taagtcagaa cagattgtgg cctgcctgtt gcgggatatc ctgtcgcagg atagttatcg
     1081 gagtatgatg tttcattcac aaatagcgct gaatcagttg gtgttgctgg agcgggggga
     1141 ttttacacac cgtgaacagt tattcatgcg taaccgggcc agttgtgact ttgttgttta
     1201 ctataaggtc gggaagactc cattaggggt tattgaagtg gatggaggtt atcatctgac
     1261 cagtgttcag gctgaacgtg atgaacttaa aaacagtata ctgaagaaat gcggcctgcc
     1321 attgctaaga ttacgtacta tagacagtga tatagaaggg aagttgggag cgtttctgtc
     1381 aggcctgaca ggctaaaatc tgaataaaaa atattgcagc gggaggtctc aaacctgatt
     1441 tggtcatgtg ccacacacaa ttgtacgaac cccatggaca caggcttctt tgttttggtt
     1501 acatcaacgt acgtattcct gatacatata aaaataagtg ctattgctca aagctaacag
     1561 ggaatgtttc tgttgaataa ttccttagtg aataaacttt atcagggggt aattctatta
     1621 cagtgaatac ttcctgcaaa atga
//
LOCUS       DAZKIF010000330         1622 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZKIF010000330 DAZKIF010000000
VERSION     DAZKIF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.333"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..195
                     /locus_tag="ACHOTW_005049"
     CDS             <1..195
                     /locus_tag="ACHOTW_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846952.1"
                     /translation="TFHDGRYRFVIESVIYDLINNYSGFILTGRLFWQGEGFGHELFP
                     VTNKYNAWRWKNKKIIDISW"
     gene            364..>1622
                     /locus_tag="ACHOTW_005050"
     CDS             364..>1622
                     /locus_tag="ACHOTW_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase toxin
                     domain-containing protein"
                     /protein_id="HFP9846953.1"
                     /translation="MTASGTGLGYGEGDESYGYDSCGYLKAQSAGGHRISEETDQYAG
                     GHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEY
                     RHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDELYSVRHLVFNGFELISQQC
                     SRVRQPHPSVAPQWVTRTNHAVNDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPAD
                     TDYPDPRGERDAEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGE
                     QTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTGFLDGVRLSRSQINNIA
                     KEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLYEVYHEVIHAKQFAKIG
                     REAYEALGRLSREEHVLNEILKSKNLF"
BASE COUNT          439 a          354 c          490 g          339 t
ORIGIN      
        1 acatttcacg atggtcgtta tagatttgta attgaatctg tgatttatga tttaattaat
       61 aattacagtg gatttatttt aacggggaga ttgttttggc aaggggaagg ttttggccat
      121 gaattattcc ccgtgacaaa caaatataat gcttggagat ggaaaaataa aaaaataata
      181 gatatttctt ggtaaggaaa atattccccg aaagaggcca catggggaat actgctcccc
      241 tcactgatac gggagtaccg ttacgacagc gcgggtaacg tatcaggcgt caccagccgg
      301 gaagattacg gacgggaaac acagcgggag taccggcttg accggaacgg ccaggtcacg
      361 gcggtgacag cctcaggcac cgggctgggc tatggcgaag gcgatgagtc ctatggctat
      421 gacagctgtg gctacctgaa ggcgcagtct gcgggcgggc accggataag tgaagagact
      481 gaccagtatg ccggaggcca ccggctgaaa caggccggaa acacgcagta tgactatgac
      541 gccgcaggcc ggatggtcag ccgcaccaga caccgtgacg gctatcgccc ggaaacagag
      601 cggttccggt gggacagccg ggaccagctg accgggtatt gcagcgcaca gggtgagcag
      661 tgggaatacc gccacgacgc cagcggcaga cgaacagaga aacgctgcga ccggaagaaa
      721 atccgcttca cgtacctgtg ggacggcgac agtattgcgg aaatccggga ataccgcgat
      781 gatgaactgt acagcgtacg gcacctggtg tttaacggct ttgagctgat aagccagcag
      841 tgcagccggg tacggcagcc gcacccgtcc gtggccccgc agtgggtgac acgaacgaat
      901 catgcggtga acgacctgac gggccgtccg ctgatgctct ttaacagtga aggtaaaacc
      961 gtctggcggc cgggacagac cagcctgtgg gggctggcac tcagcctgcc cgcagacaca
     1021 gactacccgg acccgcgcgg ggagcgggac gcggaagccg accccggcct gctgtatgca
     1081 ggacagtggc aggatgcaga atcggggttg tgctataacc ggttccgata ttacgagccg
     1141 gaaaccggga tgtacctggt gagtgatcca ctggggttgc agggagggga gcagacttac
     1201 cggtatgtgc cgaatccttg tgggtgggtt gatccgctgg gattggctgc aagttctaaa
     1261 atcagcagtt tgatggacta tattggcgat ggtcgtcgtg ttagtgggca tacgggtttc
     1321 ctggatgggg ttcgtttatc acgtagtcaa ataaacaata ttgctaaaga aatggagaag
     1381 ctaggaatta aagtaataag gaaagcagat aaatatttgc caccaaatgc tagggcagct
     1441 tttgattatg gtcttcgcaa tatttatctt aggaaaaatg ctaccttata tgaggtgtat
     1501 catgaagtga ttcatgctaa gcaatttgcg aaaattggac gagaagcata cgaagcacta
     1561 ggacgtttat ctagggagga acacgttcta aatgaaatat taaaaagtaa aaatttattc
     1621 aa
//
LOCUS       DAZKIF010000331         1618 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZKIF010000331 DAZKIF010000000
VERSION     DAZKIF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.334"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1476
                     /locus_tag="ACHOTW_005051"
     rRNA            <1..1476
                     /locus_tag="ACHOTW_005051"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          408 a          367 c          500 g          343 t
ORIGIN      
        1 ggagaaggct atgttggccg ggcgacggtt gtcccggttt aagcgtgtag gctggttttc
       61 caggcaaatc cggaaaatca aggctgaggc gtgatgacga ggcactacgg tgctgaagca
      121 acaaatgccc tgcttccagg aaaagcctct aagcatcagg taacatcaaa tcgtacccca
      181 aaccgacaca ggtggtcagg tagagaatac caaggcgctt gagagaactc gggtgaagga
      241 actaggcaaa atggtgccgt aacttcggga gaaggcacgc tgatatgtag gtgaagtccc
      301 tcgcggatgg agctgaaatc agtcgaagat accagctggc tgcaactgtt tattaaaaac
      361 acagcactgt gcaaacacga aagtggacgt atacggtgtg acgcctgccc ggtgccggaa
      421 ggttaattga tggggtcagc gcaagcgaag ctcttgatcg aagccccggt aaacggcggc
      481 cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg
      541 aatggcgtaa tgatggccag gctgtctcca cccgagactc agtgaaattg aactcgctgt
      601 gaagatgcag tgtacccgcg gcaagacgga aagaccccgt gaacctttac tatagcttga
      661 cactgaacat tgagccttga tgtgtaggat aggtgggagg ctttgaagtg tggacgccag
      721 tctgcatgga gccgaccttg aaataccacc ctttaatgtt tgatgttcta acgtggaccc
      781 gtgatccggg ttgcggacag tgtctggtgg gtagtttgac tggggcggtc tcctcctaaa
      841 gagtaacgga ggagcacgaa ggttggctaa tcctggtcgg acatcaggag gttagtgcaa
      901 tggcataagc cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa gcaggtcata
      961 gtgatccggt ggttctgaat ggaagggcca tcgctcaacg gataaaaggt actccgggga
     1021 taacaggctg ataccgccca agagttcata tcgacggcgg tgtttggcac ctcgatgtcg
     1081 gctcatcaca tcctggggct gaagtaggtc ccaagggtat ggctgttcgc catttaaagt
     1141 ggtacgcgag ctgggtttag aacgtcgtga gacagttcgg tccctatctg ccgtgggcgc
     1201 tggagaactg aggggggctg ctcctagtac gagaggaccg gagtggacgc atcactggtg
     1261 ttcgggttgt catgccaatg gcactgcccg gtagctaaat gcggaagaga taagtgctga
     1321 aagcatctaa gcacgaaact tgccccgaga tgagttctcc ctgacccttt aagggtcctg
     1381 aaggaacgtt gaagacgacg acgttgatag gccgggtgtg taagcgcagc gatgcgttga
     1441 gctaaccggt actaatgaac cgtgaggctt aaccttacaa cgccgaaggt gttttggcgg
     1501 atgagagaag attttcggcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     1561 acagaatttg cctggcggcc ttagcgcggt ggtcccacct gaccccatgc cgaactca
//
LOCUS       DAZKIF010000332         1559 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZKIF010000332 DAZKIF010000000
VERSION     DAZKIF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.336"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..370
                     /gene="escR"
                     /locus_tag="ACHOTW_005052"
     CDS             <1..370
                     /gene="escR"
                     /locus_tag="ACHOTW_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFP9846954.1"
                     /translation="QKVDEKILSPYRGFLEKNTEKDNVEFFERAAQKKLGNETILKKD
                     SLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISIPFKI
                     LLFILVGGWQKLFEFLLVVN"
     gene            370..639
                     /gene="escS"
                     /locus_tag="ACHOTW_005053"
     CDS             370..639
                     /gene="escS"
                     /locus_tag="ACHOTW_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFP9846955.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            639..1415
                     /gene="escT"
                     /locus_tag="ACHOTW_005054"
     CDS             639..1415
                     /gene="escT"
                     /locus_tag="ACHOTW_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFP9846956.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYEILPLQAD
                     IAFNRALIDFLFSLWDSFIKLMLSFSVPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            1408..>1559
                     /locus_tag="ACHOTW_005055"
     CDS             1408..>1559
                     /locus_tag="ACHOTW_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFP9846957.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GMSFFV"
BASE COUNT          462 a          256 c          281 g          560 t
ORIGIN      
        1 tcaaaaagtt gatgagaaaa tattatcacc atatcgcggt tttttagaaa aaaatacaga
       61 gaaagacaat gtagagtttt ttgaacgtgc agctcaaaaa aaattgggta atgaaactat
      121 cttaaaaaaa gactctctat ttatactgtt accggctttc acgatggggc agcttgaagc
      181 tgcattcaag ataggttttt tgctctattt accctttatt gcgatagatt tgatcatttc
      241 caatatctta ttggccttgg gtatgatgat ggtatcgcca gtaacaattt cgattccttt
      301 taagatctta ttattcattc ttgttggagg ctggcaaaaa ttattcgaat ttctgttggt
      361 ggtgaattga tggatactgg atattttgtt caattatgtg tgcaaacgtt ctggataata
      421 tttatcctct cattgcctac agtcatagcg gcctctgtta tcggtattat tattagtctg
      481 gtccaggcta taacgcagtt acaggatcaa acattgcctt ttttgctaaa aataatagca
      541 gtgtttgcta cgcttgccct gacttatcac tggatgggaa caacaatcat caacttctcg
      601 tcgataatat ttgaaatgat tccgaaggtg aacggctaat gaatgagata atgacggtca
      661 tagtatcatc attttattgt atactcaggc cgctggggat gtttattatc ctaccaatat
      721 tttcaacagg tgttttactc agtaacttta ttcgcaactc aattatgatt gcgtttacgc
      781 ttccgatcat agtagagaac tatacatttt cggaaaaatt gccatctgga atctttcagt
      841 tgacaggaat agcgctaaaa gaaataagta ttggtttttt cattgggtta tcatttacta
      901 ttcttttttg ggcaatagat gcggctggac agattattga tactctaaga ggctcaacaa
      961 tatcttcaat ttttaacccg tccataagtg attcatcttc tatcactggc gttatcttgt
     1021 accaatttat ctctgtgatc tttgttattc atggtgggat acaaagcatc ctagataagc
     1081 tatatttatc ctacgagata ttaccattac aagccgatat tgcattcaat cgtgctttaa
     1141 tagatttttt gttttctcta tgggattcat ttattaaact gatgttatca ttttcagttc
     1201 ccatgattat cggtatattc ttatgtgata tggggtttgg gtttcttaac aaaacagcac
     1261 ctcagctaaa cgtattcaca ttatcactgc cagtaaaaag tttgatagca atctttatat
     1321 tgctattagt tattcatgtt tttcctgatt ttattacggc aaatattcat tctgacataa
     1381 ttgatagatc gttaccgagc atgattaatg agtgaaaaaa cagaaaagcc cacacccaaa
     1441 aaactaaggg atctaaaaaa gaagggcgat gtaacaaaaa gtgaagaggt aatggctgca
     1501 gtgcagtcat taatcttatt ttcatttttt tctttatatg gcatgagttt ttttgttga
//
LOCUS       DAZKIF010000333         1546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZKIF010000333 DAZKIF010000000
VERSION     DAZKIF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.337"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(66..1472)
                     /gene="gndA"
                     /locus_tag="ACHOTW_005056"
     CDS             complement(66..1472)
                     /gene="gndA"
                     /locus_tag="ACHOTW_005056"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9846958.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          388 a          390 c          375 g          393 t
ORIGIN      
        1 aattggcaaa aaaaagcccg gtacaatata cgccgggcct caattttatt gttgattaaa
       61 tcagattaat ccagccattc ggtatggaac acaccttctt tatcaatgcg cttataagta
      121 tgcgcaccga aatagtcacg ctgtgcctgg atcaggttcg caggcagaac agcggcgcgg
      181 tagctgtcat aataggcaac cgcagcggcg aaggtcggaa ccgggatacc gttctgtacc
      241 gcataagcga cgacatcgcg cagcgcctgc tggtagtcat cggcaatttg cttgaagtaa
      301 ggagccagca gcaggttagc gatctgcgga ttttcggcat aagcatcggt gattttctgc
      361 aggaactgcg cacggatgat gcagccagca cggaaaatct tcgcgatttc gccgtagttc
      421 agatcccagt tgtactcttc agacgccgca cgcagttgag agaacccctg agcgtaagaa
      481 acgattttgc ccagatacag tgcacggcga actttttcga tgaactcagc cttgtcgcca
      541 gctggctgcg cttgtgggcc agagagaact ttagacgcag caacgcgctg atctttcaga
      601 gaagagatgt atcgtgcaaa cacagactcg gtaatcagcg acagcggttc gccgagatcc
      661 agtgcgctct ggctggtcca tttgcccgta cctttgtttg ccgcttcatc caggatcaca
      721 tcaaccaggt agttaccgtc ttcatctttt ttagtgaaga tgtctttggt aatgtcgatc
      781 aggtagctgc tcagttcacc gttattccac tcggtaaaga tctgcgccag ttcttcgttg
      841 gtgaggttca gaccaccttt aagcagagaa taggcttcag caatcagctg catatcgccg
      901 tattcaatac cgttgtgaac catcttcaca tagtgacctg cgccatcggc accaatatag
      961 gtaacgcatg gctcaccgtc ttcagccact gcggcgattt tggtcaggat cggcgcaact
     1021 agttcatagg cttctttctg cccaccaggc ataatggaag gaccttttag tgcgccctcc
     1081 tcaccaccgg aaacaccggt accgatgaag ttaaagcctt ctgcagaaag ctcacggtta
     1141 cgacgaatgg tgtcctggaa gaaggtatta ccaccatcaa tgatgatgtc acctttatcg
     1201 aggtatggct taagggaatc aatagcagca tccgtgcctg cacctgcttt caccattaac
     1261 aagatgcgac gaggcgtttc cagagattca acaaattctt tcaccgtata gtaaggaacc
     1321 agtttcttgc ctggattttc ggcaatcact tcttccgtct tttcacggga acggttgaaa
     1381 atagagacgg tataaccacg gctttcgatg ttgagcgcaa ggttgcgccc catcactgcc
     1441 ataccgacta cgccgatctg ttgctttgac attgtttact cctgtcaggg gttaccgcta
     1501 gatatgcggc ttgaataacg tataaaaatg tatcagagta ttataa
//
LOCUS       DAZKIF010000334         1529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZKIF010000334 DAZKIF010000000
VERSION     DAZKIF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.338"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(177..587)
                     /locus_tag="ACHOTW_005057"
     CDS             complement(177..587)
                     /locus_tag="ACHOTW_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP9846959.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            873..1079
                     /gene="ybcW"
                     /locus_tag="ACHOTW_005058"
     CDS             873..1079
                     /gene="ybcW"
                     /locus_tag="ACHOTW_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFP9846960.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(1244..1438)
                     /locus_tag="ACHOTW_005059"
     CDS             complement(1244..1438)
                     /locus_tag="ACHOTW_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9846961.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
BASE COUNT          425 a          279 c          332 g          493 t
ORIGIN      
        1 tgcgaaggat cgtcactgta ttcccggatt aactatgtcc gcagccctga cagggaaact
       61 cctctgcggg agtgtccggg aataattaat aacgatgcac acagggttta gcgcgtacac
      121 gtattgcatt atgccaacgc cccggtgctg acacggaaga aaccggacgt gatgatttag
      181 cgtggaaaga tttgtgtagt gttctgaatg ctctcagtaa atagtaatga attatcaaag
      241 gcatagtaat atcttttatg ttcgtggata tttgtaaccc atcggaaaac tcctgcttta
      301 gcaagatttt ccctgtattg ctgaaatgtg atttctcttg atttcaacct atcataggac
      361 gtttctataa gatgcgtatt tcttgagaat ttaacattca caaccttttt aagtcctttt
      421 attaacacag tgttatcgtt ttctaacaca atgtgaatat tatctgtggc tagatagtaa
      481 atataatgtg agacattgtg acgttttagt tcagaataaa acaattcaca gtttaaatct
      541 tttcgcactt gatcgaatat ttctttaaaa atggcaacct gagccattgg taaaaccttc
      601 catgtgatac gagggcgcgt agtttgcatt atcgttttta tcgtttcaat ctggtctgac
      661 ctctttgtgt tttgttgatg atttatgtca aatattagga atgttttcaa ttaatagtat
      721 tggttgcgta acaaagtgcg gtcctgctgg cattctggag ggaaatacaa ccgacagatg
      781 tatgtaaggc caacgtgctc aaatcttcat acagaaagat ttgaagtaat attttaaccg
      841 ctagatgaag agcaagcgca tggagcgaca aaatgaataa agaacaatct gctgatgatc
      901 cctccgtgga tctgattcgt gtaaaaaata tgcttaatag caccatttct atgagttacc
      961 ctgatgttgt aattgcatgt atagaacata aggtgtctct ggaagcattc agggcaattg
     1021 aggcagcgtt ggtgaagcac gataataata tgaaggatta ttccctggtg gttgactgat
     1081 caccataact gctaatcatt caaactactt aacctgtgac agagccaaca cgcagtctgt
     1141 cactgtcagg aaagtggtaa aactgcaact caattactgc aatgccctcg taattaagtg
     1201 aatttacaat atcgtcctgt tcggagggaa gaacgcggga tgttcattct tcatcacttt
     1261 taattgatgt atatgctctc ttttctgacg ttagcctccg acggcaggct tcaatgaccc
     1321 aggctgagaa atttccggac cctttttgat caagagcgat gttaatttgt tcaatcattt
     1381 ggttaggaaa gcggatgttg cgggttgttg ttctgcgggt tctgttcttc gttgacatga
     1441 ggttgccccg tattcagtgt cgctgatttg tattgtctga agttgttttt acgttaagtt
     1501 gatgcggatc aattaatacg atacctgcg
//
LOCUS       DAZKIF010000335         1529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZKIF010000335 DAZKIF010000000
VERSION     DAZKIF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.339"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            289..1233
                     /locus_tag="ACHOTW_005060"
     CDS             289..1233
                     /locus_tag="ACHOTW_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846962.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEESHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLTSDE
                     YMSILTPLMKCERSVHDNNRYVTGKDTLLLDYPPSGNQIHFHVFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTEANKIL"
     gene            1315..>1529
                     /locus_tag="ACHOTW_005061"
     CDS             1315..>1529
                     /locus_tag="ACHOTW_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846963.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDMAY
                     LSRCLASIHPTLAKNIYETENLSDQKLL"
BASE COUNT          485 a          313 c          268 g          463 t
ORIGIN      
        1 ttgattagag tagccaattt gttgctattt tacataacat atttttatca aagttaattg
       61 attgttgcct ttatgagcac cgaccgtaag cccgttatgt tattgtttca ttagtaataa
      121 tcacttaaat gtatcgatga aacaggtatg acttaccggg agtgattgtt tctgagtgaa
      181 cagaggccac tttcgcctga taaatgaaaa atgagcgatt tttgcaagtt agtttttcta
      241 tagccgagta cgctcagcgc ctcgcttgat tacaatgagg ttgctgctat gagtcctgat
      301 aatatcggtt cggctgtcaa tcgcttctca ggttggccat ctaatgattc tctaaaaaca
      361 gataaagaat caatgacatc acccgtgact gtgcaggtaa taaagatgat tgccgccatc
      421 cttgacccac atcggatagt cgttggaatt gatacacttt atacaacgca gcgcgaaaca
      481 tttacctcag agagtcaaca gctcattgaa tcagcattgc aaacacttca tgtgatgaat
      541 ttaaataatc tggaaacaaa tcgcgatgaa gaaagtcact atctggcttt tgaatataac
      601 gttgacacct ctgacagaat aaccgtttct gcttataatg aaaaaaaacc tggctgctat
      661 ggccaatgtc ttgtaataca ggatgacgaa acagacttat ttaacctgat aaaaattaaa
      721 attttcgaag ccaggcataa ttatgtcgct caattgatga gcgatccgga atttatgttc
      781 aaattcagtt atagcgctca acaaaataga ttagaacctc atcttctggt tcccgcttat
      841 ttccctctaa aaacaaacga accggttacg caagaggatg tactgttact ttatcgtttc
      901 tttaaaatga atgatgattt taataaatta acatcagatg aatacatgag tatcctgaca
      961 cctctgatga agtgtgagcg tagcgtccac gacaacaaca ggtatgtgac gggcaaagat
     1021 acccttcttc tggactatcc tccttctggt aaccagatac atttccatgt tttccctgac
     1081 gaatcagcaa cactagtcct ttatttttca aacaatgaca ttgaatgttt cgtctttgaa
     1141 agagatattc cgagccagta tcgctttttc aaaatgttta ccaatctggc actggttata
     1201 gatcagttaa ccgaagccaa taaaatactt taattttcta tatatcccct atggcaaata
     1261 ttgctaaaca atgcctgggg attaattttt attactcacg gaaagattta aataatgata
     1321 ccatctcaga tctctttcaa tacccttcca gtcaatgcaa catacgcttc agagacaaca
     1381 gttgacacac aaaaattctc tgacatcctc tactcagcgg ggtgttccct aaaggatatg
     1441 gcttatctga gcagatgtct ggcatcaata cacccgactc tcgccaaaaa catttatgaa
     1501 acagaaaatc tcagcgatca aaaattact
//
LOCUS       DAZKIF010000336         1488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZKIF010000336 DAZKIF010000000
VERSION     DAZKIF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.340"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHOTW_005062"
     CDS             complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHOTW_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9846964.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVR"
     gene            704..1477
                     /locus_tag="ACHOTW_005063"
     CDS             704..1477
                     /locus_tag="ACHOTW_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9846965.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLWQNERIIKIIKDDISESKHTKSICLLNGNLSLDYSASLYPEAIKAESV
                     LRNILTEKYSNFSIVQFPELNHQETFSAALWNSIIHFSI"
BASE COUNT          467 a          299 c          268 g          454 t
ORIGIN      
        1 tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc actatgacgg ttaaacagat
       61 gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt
      121 cggcacgtaa gaggctaacc tagatttgcc gttcatagat tgagcctcag attaatgtta
      181 ggcgtcttgc aggacgcgta atgttaactg gggcttttct ctatctgccg ttggtgttca
      241 tgcccgaggc agatagcctc aagcacccgc agctattcta cttaacttcc cattacctcg
      301 ccaatatgaa atcggtcaga aagacagacg tcccataagg acaacaacgg aaaaagagat
      361 tttgcttgat gctatttacc tctgcaccat gaagttgcgc caaaatccca gtggtgataa
      421 caggaatttg gttgttggtg attggggaaa gaagtacgtt agactgtggg ttagttcatt
      481 gacatgatag ttacctgcct tcaatttcct aagcaagcga tgtaaacgat aatcaatatc
      541 aaatgtattg acaggtaaaa taataacgat gtacctttgc tgataacata tatcagtctt
      601 aaatcataat ggaggtagat aaaataacta aacacacaca acaaattttt tatcagtgac
      661 atagcatatc ttaagcatcc tttaatgctc atggggagaa cgcatgacta aacatatgga
      721 cattcattgt caaacacgca tatttaactc aattaaccat gacaccaaat ataaaatatg
      781 tatttttact ccaaaaaaca ttaaaaaaaa taatgcaact ctttatatac ttgatgggaa
      841 tagtgccaac aattacattt ctgatattct gcctgttatt gatgcactac ccaatccacc
      901 agtgttagtt acacttggtt atgaatcttg gaataacctt agcattcatc gccgtgctta
      961 cgattatact cctgatggtg aaaatgctat tgttgataac tctaaaccag catggatcta
     1021 ctttactggt ggaggaagcc agtctttccg agaattatta ctaactcaga tcatgccatg
     1081 ggttagtact attgctccga acagttccag aatcggtata tggggacact cgctaggtgc
     1141 tatttttgtg cttgattgct taaaaaataa ttcatgcttt aactattatt atatatctgc
     1201 accatcactt ctatggcaga atgaaagaat catcaagata attaaagatg atatatcaga
     1261 atcaaagcac actaaaagta tatgcttgct taacggaaat cttagccttg attactccgc
     1321 atctttatat cctgaagcca tcaaagcgga atctgttttg agaaatattt tgacagaaaa
     1381 atacagtaat ttttctattg tacaatttcc agagctcaat catcaggaga ctttctctgc
     1441 tgcactctgg aatagcatta ttcatttcag catataacag aaaatatg
//
LOCUS       DAZKIF010000337         1477 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZKIF010000337 DAZKIF010000000
VERSION     DAZKIF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.341"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            172..>1477
                     /gene="espK"
                     /locus_tag="ACHOTW_005064"
     CDS             172..>1477
                     /gene="espK"
                     /locus_tag="ACHOTW_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFP9846966.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNNQIMKNIKFYLHGKEIPAERILDT"
BASE COUNT          521 a          311 c          272 g          373 t
ORIGIN      
        1 gactggagaa agtattcgca aaagttaaaa tcaacagtct gttggttttg agatataact
       61 ctcttttcta atattgacac cttggctttg ctggttgtta aaattagctt gttgattagt
      121 agtatggata gaacttactc tgttatttat ttctaaaaat ggaatttatc aatgcttcct
      181 acatcgcaat tacgaccgac cgggacattc tgctcctatt ccgctgaaac atcagcagac
      241 atcaaaagcg aaatcacacc aattcagata gaagaagcgc gggccagtgg tcgtttatat
      301 atcaaagatt gtgatattga gtatctgcca cagttaccaa acgaaataac atcagttaca
      361 atcgaaaact gcaacaacct gacaaccctt acaggattgc cggttaatac acaaaacctc
      421 tccgtcatta actgtgaaaa attacaaatc acagacatgc catcaaccgt aaaaaatcta
      481 catattgaat taactgattc accatttata catttcatat ctgaaggcat cgagtgcctg
      541 acggtttgcc actgctatat atctggagtg ccagagagtg tccgctacct tgaaataaaa
      601 ggtagcgcca cagacagcat aaaaaatgtt ccaaacgggt tatcatctct cagcatcaat
      661 agctataacc cggagaatca ggccagaatt gacaacctga tatcaccgtc actgaagacg
      721 ctatcgctga ctggatgtag caatattata ctgccggaga aacttccgga gagtgtgaca
      781 tcggtaacca ttcatgcgga gcagaaaacc acgtggaaca tcggtgttga agggatgcct
      841 gatgggctgg atcttgattt acaaaatgta ctactctctc cagatgtagt taaagcaaaa
      901 aacatcacct ttcagggcaa cgctctggat gtggccttac actttcgcga gggagacatt
      961 gtctatggac tatcttcacc cagagaaaaa cttgtcaaca gcattaaact agttaacgac
     1021 ttttccaaaa aagatattat aactcagaat acgttaacaa acgcagtatg ggaccccaga
     1081 acacctcgca aatataagca agatccactt atcaaaagag cattaaatga acacgaaaga
     1141 ggaataaaat ttaaacaaca cttaaagaat cacaataatt ataatgttac catggccgac
     1201 ctttccgtat acaatcgcga caaattatgg gcaaaaacaa gcaaggccgg cctagagttt
     1261 cagacattaa cacgcaataa aacggttatt ttttgtgcgg atgagcttgt caactcactc
     1321 aaactcatag ctaacaagtc agagggctat ggccagagta ttaccgccag cgaattacga
     1381 tggatttacc gtaataaaga caacaaccaa ataatgaaaa acataaaatt ttatctacat
     1441 ggcaaagaga taccagcaga aagaatatta gatacac
//
LOCUS       DAZKIF010000338         1439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZKIF010000338 DAZKIF010000000
VERSION     DAZKIF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.342"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..515
                     /locus_tag="ACHOTW_005065"
     CDS             <1..515
                     /locus_tag="ACHOTW_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFP9846967.1"
                     /translation="FMKDMKMDKQEVKREYKEQEGNPEIKSKRRERHQEILSEQLKSD
                     VSNSRLMIANPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIGIPIITDKK
                     LARKIYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPVPDEQLSSEDKYIEGEDTK
                     SENNDNNLKN"
     gene            complement(576..842)
                     /locus_tag="ACHOTW_005066"
     CDS             complement(576..842)
                     /locus_tag="ACHOTW_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFP9846968.1"
                     /translation="MIITIALISLLSACTRENSLKENQNKRSGESQAIVDARQFFSEH
                     PEYISSAELEEKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
     gene            complement(872..1372)
                     /locus_tag="ACHOTW_005067"
     CDS             complement(872..1372)
                     /locus_tag="ACHOTW_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9846969.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIWINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNQLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
BASE COUNT          496 a          228 c          259 g          456 t
ORIGIN      
        1 tatttatgaa agatatgaaa atggataaac aagaagttaa aagagaatac aaggaacaag
       61 aaggaaatcc tgaaattaag tctaaacgca gagagcgcca tcaggaaatt ctttctgagc
      121 aattgaaatc tgatgtcagt aatagccgtt tgatgattgc caaccctact cacattgcaa
      181 tagggatata ctttaagcca catctgtcac ctattccatt gatttctgta agagaaacta
      241 atgaggtagc attagctgta aggaaatatg caaaggaaat cgggatacca attattacag
      301 ataaaaaatt agcacgaaaa atttatgcta cccatcgtcg ctacgattat gttagcttcg
      361 aaaatataga tgaaatatta cgtcttctgc tgtggcttga agatgtggag aatgctggac
      421 aacctgttcc agatgaacag ctctcttcag aagataaata tattgagggt gaagacacaa
      481 aaagcgagaa taatgacaat aatttaaaaa attaaacatg tatcgtagtc aaaaatttat
      541 aagaatctac acgcaaaaaa cgtgtagatt tttatttaat gagttaaacg atcttgcgaa
      601 atgacaagca attgatacat actcatctct ttataagctg gaactgtagt aacctgtgca
      661 aattcgcgat atagcttttc ctccagttct gctgatgata tatattccgg gtgttcgcta
      721 aagaactgtc ttgcatctac tatcgcttgg gattcgccac ttcttttatt ttgattctcc
      781 tttaatgaat tttctctggt gcatgcagag agcaacgata tcagcgcgat ggttattatg
      841 attttactat tatatcatta ctaatgacat atcaactttc tcttacgcaa gattggagtt
      901 taaaataatg tttatcccat tcacagagtg ctataaacaa ctcaacagta ccttttatgt
      961 taagttgatt gagggctctt attttgtgat atgatactgt tttataatca attcctttaa
     1021 taatggcaat ttgctttaat gaatatgaaa cacataaccg caggatatct atctcatcat
     1081 gggtaagtgg ttcttgtgtg gaatacacat tttgtgtatg aagatttgaa accaggaaat
     1141 gcgttaaatc agataatgta atattcttat tgatccagat gaaattgtta ctcaaaagga
     1201 tgtttacagg ttctgtattt ggtttgagat ttcgatgttg atgctcatca ataacaacga
     1261 acgaatgtcc aagatcaacc aacgcaacta aatcgctgta atattccttt tgggatgcta
     1321 ataatgcatt agtgcctaca gtgaagtaga catcactgga aaagacttgc atatgccaac
     1381 ctgtactcta aaatcattta gggtaaatat tatgtgtaaa atttaaaata agagtaatt
//
LOCUS       DAZKIF010000339         1438 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZKIF010000339 DAZKIF010000000
VERSION     DAZKIF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.343"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(95..424)
                     /locus_tag="ACHOTW_005068"
                     /pseudo
     CDS             complement(95..424)
                     /locus_tag="ACHOTW_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(382..592)
                     /locus_tag="ACHOTW_005069"
                     /pseudo
     CDS             complement(382..592)
                     /locus_tag="ACHOTW_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140296.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            776..>1438
                     /gene="espR3"
                     /locus_tag="ACHOTW_005070"
     CDS             776..>1438
                     /gene="espR3"
                     /locus_tag="ACHOTW_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR3"
                     /protein_id="HFP9846970.1"
                     /translation="MFPLNDLSLKTQSVQLNKITSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNDKFTPVSEDSNLLNMLSEFKLLREQCFRWGNYTLLFENYGAYDKTGSITI
                     EKSQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGASNDLKE
                     NVDRALVRMFKCVKLHSNELNLSSLSLGSVPPLPEWIEMLSLVYNELDSIQVPESCKE
                     LEL"
BASE COUNT          429 a          275 c          316 g          418 t
ORIGIN      
        1 acgcatgtgg aaggagcgtc tatggacaag gcgtttcctg ctggtgataa ataaaggcgc
       61 agcacgtcga atgcaagaat gcgctgcgtc tggctcatgg caattcatta tcttcgcata
      121 gcgtcaatga caaaaatcga ctgtgtggat caaattgata atcagcaaac ggttcgcaat
      181 caacgaaacc atgcttcaaa taaagttgat ggcaggccgt aaatccagcc tgtgtacccg
      241 tttctaagct caggcgatga aggcatctgt catgggcgac ctgcaaaatg tggcgtaaaa
      301 tcagactggc gacaccccga cgtaaataat ttggcgcggt acgcattgat ttcagttcgc
      361 catgtttatc atccagcaac tttagcgcac ccattcctgc gagttgttcg ccttcccata
      421 ctgacctgtt gacctggctt gtttaatgac atccgatctc accgcgtggg gttatggatg
      481 gggcaaactc actcaatttc tggtttaaaa tggaaacctg attctgaaag ttttctgcca
      541 cccacgatcc gtatacgcga taaaccctct gatcatcaga atgtctcatt tgacacatca
      601 ccacgtcgga tgttgttacg accacagacg caacagtaac ctctggataa cttaatcttc
      661 gttcttattt ttattaatat actgcgttat aaaaatgagc gatttttgat agtggcaatt
      721 ttattgtgtt ttagaatgcg ccctgggcaa tataatttat taaacaggag tgtaaatgtt
      781 tccattaaat gatctgtcgt taaaaactca gtcggttcaa ttaaataaaa tcacatcgaa
      841 tactgagtct acgataaaac aacacgagtt agtatctgat gatgcaatca taaatgaatt
      901 atcaagtgag ttagtcagtt gtcttggaaa tgataagttt acaccagtta gtgaagacag
      961 caacttactg aatatgctgt ctgaatttaa gttattgaga gagcaatgtt tcaggtgggg
     1021 taattatact ctattgtttg aaaattatgg ggcttatgat aagacgggat ctatcacgat
     1081 agaaaaaagt cagggggagg ggactttacc cattcggcat aaattagagt ttatatcgac
     1141 caatattgca gagttgctgg acaagttaac caaaattaca gatgccaggc tttgcaaagg
     1201 tttcagtgac tgggctagtt cagtcaaaga aggcgcatcg aatgacttga aagaaaatgt
     1261 ggatagagca ttggtgagaa tgtttaaatg tgttaagctt cacagtaatg aacttaactt
     1321 atcaagcctt tctttgggtt ctgtgccgcc tcttcctgag tggattgaaa tgcttagcct
     1381 tgtttataat gaacttgatt caatacaggt gcccgaatcg tgcaaagaat tagaactc
//
LOCUS       DAZKIF010000340         1431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZKIF010000340 DAZKIF010000000
VERSION     DAZKIF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.344"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1182
                     /locus_tag="ACHOTW_005071"
     CDS             <1..1182
                     /locus_tag="ACHOTW_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9846971.1"
                     /translation="RRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALI
                     SEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGR
                     YITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSG
                     DLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSG
                     VSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWV
                     N"
     gene            1194..1379
                     /locus_tag="ACHOTW_005072"
     CDS             1194..1379
                     /locus_tag="ACHOTW_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846972.1"
                     /translation="MKKYIFLVLFLLYGCSPVDIEPARQEVNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKNLST"
BASE COUNT          394 a          280 c          424 g          333 t
ORIGIN      
        1 cggcgaatgg caaaacgggt ctggcggcgg gagcgtgacc tgacggggtg gatgtcgctg
       61 tcgcgtaaac cggaggtgac gtggtatggc tgggacggag acaggctgac gacggtgcag
      121 actgacacca cacgtatcca gacggtatac gagccgggaa gcttcacgcc gctcatccgg
      181 gtcgagacag agaacggcga gcgggaaaaa gcgcagcggc gcagcctggc agagacgctc
      241 cagcaggaag ggagtgagaa cggccacggc gtggtgttcc cggctgaact ggtgcggctg
      301 ctggacaggc tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
      361 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
      421 ccggcgcgaa aagttcattt ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
      481 agcgaagacg gcaatacggc gtggcgcggg gagtatgatg aatggggcaa ccagcttaat
      541 gaagagaacc cgtattacct gcaccagcca taccgtctgc cggggcagca gcatgatgag
      601 gaatcagggc tgtactataa ccggaaccgg tactatgacc cgctacaggg gaggtatatt
      661 acacaagacc ccattgggct ggcgggggga tggaatctgt ataattaccc actgaatccg
      721 ataataagga tggatccttt gggtttgtat aatttatatc aattattata tgatgtttgg
      781 catgatgatt catatggaac atcatcaatt gatattactg gcagtggaga tctaatatca
      841 ttaggtggtc atgcaggact tggcgttgcg tttgctaaaa agaaaggtga aatgttatct
      901 gatatttgta tttatgctac agcatgcgga catgcaggaa ttggtggtgg gataaatgcg
      961 gctatcacat attctgagac caagtcttta cctacatcgg gagtcagcaa ttcagtaggt
     1021 gtaacggttg gcggcggagt tggggggcat tttgcgtata cttatgtagt ggatgttgat
     1081 aatccagaat catcgacaga atctgttggt atcggtgcag gtgttgacgc ttcagttatg
     1141 actctggctt gtagaacgtg gcaagaatgc tgggtcaatt aacaggagga aatatgaaaa
     1201 aatatatatt tttagtttta tttttattgt atggttgttc tccagtggac atagagccag
     1261 ctagacaaga agttaatatc tttcgtggtt tatatcagac agggaaatac agtgatatat
     1321 ataaaatcac ttcaagtgat tttcagactg ccacatctga aaaaaattta tcgacataat
     1381 gaacgaagca aaagaaaaag atcttgggac ttataaaaaa tcaaccctta a
//
LOCUS       DAZKIF010000341         1409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZKIF010000341 DAZKIF010000000
VERSION     DAZKIF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.345"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            46..1409
                     /locus_tag="ACHOTW_005073"
                     /pseudo
     CDS             46..1409
                     /locus_tag="ACHOTW_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          503 a          280 c          253 g          373 t
ORIGIN      
        1 gtttccgtat acatcatatt actctaccat tagaggaacg ttattatgtt ttctataaaa
       61 ccaggaccaa gaaaattacc tatcgacaac ccctcattgt tatcatggaa cattactgac
      121 ggggatctaa attccaaatt aaatacatta gaatatctaa actgtgtaac aaatattatt
      181 aatgcttgtg gagtttatcc tcaggattta aaagacagag aaattatatc aacttttcac
      241 gcagaaaaag tcattaatga tctgttaaaa aacgattata aaatttccct ttctccagat
      301 acaacttacc gagagctgaa taaagctgca cagcgtagca ttacagcgcc agacaggata
      361 ggagaaggga aaatatgggt ttatcaacga gatacaatgg ttgaaagagg tgataacagc
      421 ggtgttcatc agtatggtcc agctgaacat ttcactcaca ttatatctga caaaccgtcc
      481 ccaaaagata agtatgttgc atatgctatt aacattcctg actatgagct agcagccgat
      541 gtatataata ttaacgtgac gtcaccttcc ggacagcaag aaacatttaa aatattaatc
      601 aatccagaac atctacggca aacacttgag cgtaaatccc ttactgctgt tcagaaatca
      661 caatgtgaaa tcatcacccc caaaaaacct ggcgaagcga ttcttcatgc ttttaatgcc
      721 acctaccagc aaatcaggga aaatatgtct gagtttgcac gttgccatta tgggtatata
      781 caaatccctc cagtgacaac tttccgcgcc gacggaccag aaactcccga agaagaaaaa
      841 ggttactggt ttcacgctta tcaacccgaa gatctttgta ccatccataa tccaatggga
      901 gatttgcagg attttatcgc attggttaaa gatgctaaaa aatttggtat cgatatcatt
      961 cctgattata cctttaactt tatgggaatc gggggtagtg gtaaaaatga cctggattat
     1021 ccctctgctg atatacgagc gaagatcagt aaagatatag aaggtggtat ccctggctat
     1081 tggcaaggtc aggttttgat tccatttact atagatccag taacaaaaga acgtaaacaa
     1141 atccatccag aagatataca tctcacagca aaagacttcg aagcaagtaa agataatatt
     1201 tctaaggatg aatgggaaaa cctccatgca ttaaaagaaa agcgtttaaa tggaatgcct
     1261 aaaacccccc caaaagtgac taggttatta tgttgcaaaa tcaatacgtt cgtgaaatgc
     1321 gaaaatatgg cgtacgaggt ttacgttacg atgcagcaaa acactcaaaa catgaacaaa
     1381 tagaaagatc aataacccca ccgcttaaa
//
LOCUS       DAZKIF010000342         1394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZKIF010000342 DAZKIF010000000
VERSION     DAZKIF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.346"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            162..>1394
                     /gene="pitB"
                     /locus_tag="ACHOTW_005074"
     CDS             162..>1394
                     /gene="pitB"
                     /locus_tag="ACHOTW_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFP9846973.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPGNTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGE"
BASE COUNT          319 a          331 c          360 g          384 t
ORIGIN      
        1 tgttctaatt atttctaaag atttttaatg aatgcttatt gtctgataca caagaaataa
       61 cactcttttt atcgttaaaa aatgatattt cactttgccc acaccgttaa agtcactgat
      121 aatgcgtccg ttcgtaaatt caaaatggcg taatctaata tatgctaaat ttatttgttg
      181 gccttgatat atacacaggg cttttgttat tacttgctct ggcatttgtg ttgttctacg
      241 aagcaatcaa tggttttcat gacacggcga atgcggtggc aaccgttatt tatactcgtg
      301 ccatgcagcc acagcttgcc gtggtgatgg cggcattttt taactttttt ggcgtgttat
      361 tgggcggact tagcgttgcc tatgccattg tccatatgtt gccaaccgat ttgttgctga
      421 atatggggtc aacccacggc ctggcgatgg tcttttccat gctgctggcg gcgattatct
      481 ggaacctggg aacgtggttc tttggtttac cggcctccag ttcgcatacc ttgattggcg
      541 cgattatcgg catcggttta accaacgcgc tgttaaccgg ctcatcggtg atggatgcgt
      601 taaacctgcg tgaagtgacc aaaattttct cctcgctgat tgtttcccct atcgtcggcc
      661 tggtcattgc gggaggcctg atattcctgc tgcgacgcta ctggagcgga acgaaaaagc
      721 gtgaccgtat tcaccgcatt ccggaagatc gcaaaaagaa aaaaggcaaa cgtaagccgc
      781 cattctggac gcgtattgcg ctgattgttt ccgctgcggg cgtggcgttt tcgcacggcg
      841 cgaacgacgg acaaaaaggg atcggcctgg tgatgctggt actggtgggg attgcccctg
      901 ctggcttcgt cgtcaatatg aacgcgtccg gctatgaaat tacccgtacc cgcgatgccg
      961 ttaccaactt cgaacactac ctgcaacagc atcctgaact gccgcagaag ttgattgcga
     1021 tggaacctcc attgcctgcg gcatctactg atggcgcgca agtgacggag tttcactgtc
     1081 atccgggaaa tacctttgat gcgattgcgc gcgttaaaac gatgctgcca ggcaatatgg
     1141 aaagttacga gccgttaagc gtgagtcagc gcagccagct gcgccgcatt atgctgtgca
     1201 tctctgatac ttccgcgaag ctggcgaaac tgccaggcgt cagtaaagaa gaccagaacc
     1261 tgctgaaaaa actgcgcagc gatatgttaa gcaccattga gtacgctccg gtgtggatca
     1321 tcatggcagt agcactggcg ctcggcattg gcaccatgat tggctggcgt cgtgttgcga
     1381 tgaccatcgg tgag
//
LOCUS       DAZKIF010000343         1389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZKIF010000343 DAZKIF010000000
VERSION     DAZKIF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.347"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            543..734
                     /locus_tag="ACHOTW_005075"
     CDS             543..734
                     /locus_tag="ACHOTW_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846974.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
     gene            complement(804..944)
                     /gene="ykgO"
                     /locus_tag="ACHOTW_005076"
     CDS             complement(804..944)
                     /gene="ykgO"
                     /locus_tag="ACHOTW_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP9846975.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(944..1207)
                     /gene="ykgM"
                     /locus_tag="ACHOTW_005077"
     CDS             complement(944..1207)
                     /gene="ykgM"
                     /locus_tag="ACHOTW_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9846976.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          413 a          282 c          303 g          391 t
ORIGIN      
        1 gaaaaagaca ttcagtttca taaggttatt ggcaatataa cgttcaattc ttacttgcat
       61 tttatattag ccaggaactt tgccctgagg tgttaggaat agtcttaatg tgttgtgtaa
      121 tggactagta aaaaaacaag tctaaatgat aagaaataga atatgaaact atcatgagtc
      181 ataattaaag atgatttgcc aatcagtaag gtgttttaat ggattaacat gccgcacaaa
      241 atggttatgg gtgttcatcc caataagtga tgaaaaatta aacaggtgta agtacgtcat
      301 ctgtccggta agccccctaa gagccagatt aagtgaagtt aaacgaagac tgccagttgt
      361 caccggaacg atggcctctt tgccagggca ctgcattcag tcatctgtgc gcgttactct
      421 aactggcact cgaacgtcgg cgacggggtt accggtggat caggccgttc ctgtggcgta
      481 aacgttttta tgtcagtacc aacagattta ccacatttat catcttaaca gtttgagtgt
      541 aaatgcaggc tgcgtcgcaa agggaggaaa ctgagtggcg tgttcagtct aaaagagggt
      601 tgatgccagc atacaggggc gaagctggtc aacaagtcaa cattaatata atggaatatt
      661 cggagagaaa cgtcaggcag ttgaccttaa atgaacagga agatacatcc ccaggaaaat
      721 taatgctggt gtaataaata caccaacatt aatccggtct gattattaat cagacaaata
      781 tcgttaattc gtcgaatttt aaatcaacgt tttttcttac gaccctgaac ggccttaaag
      841 cgtggattag atttacaaat cacatatagc cgtccttttc gcttcacaat ctgacagtct
      901 ggatggcgtt cttttgcggt acgcagagag ttaaggactt tcatcacgcc ccctttttcg
      961 tgctaacaaa acgaccaaaa cgttgggtga atcgtgcaac atttccttct gatgccactg
     1021 ttctcagctt ccctgtatag aacgggtgcg atttcgaaga gacatcaatt gttacgtacg
     1081 gatacgttac gccatccagc tcaatctcac ggtctgtttt gatagtcgag ccgattttaa
     1141 agtactcatc aacactggtg tcgtggaaca ccacagtacg atactcagga tggatattgg
     1201 gcttcatcat ttttacctgt tatgttataa cataaccaca aagatacatg ctcatccgga
     1261 tcatttcaac acccaatccc tgtgaccatt agtggtatgt ggaatttcct caacggggaa
     1321 gtctggcatt gttaacagcc attggcgacg ctcccataaa cgcctggatg agtgattggt
     1381 aagcgtaca
//
LOCUS       DAZKIF010000344         1384 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZKIF010000344 DAZKIF010000000
VERSION     DAZKIF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.348"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..66
                     /locus_tag="ACHOTW_005078"
     CDS             <1..66
                     /locus_tag="ACHOTW_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846977.1"
                     /translation="VSLIHLDASAGCLPGLPTFNW"
     gene            78..275
                     /locus_tag="ACHOTW_005079"
     CDS             78..275
                     /locus_tag="ACHOTW_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9846978.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFDNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            360..701
                     /locus_tag="ACHOTW_005080"
     CDS             360..701
                     /locus_tag="ACHOTW_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846979.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            762..905
                     /locus_tag="ACHOTW_005081"
     CDS             762..905
                     /locus_tag="ACHOTW_005081"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846980.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            899..1318
                     /locus_tag="ACHOTW_005082"
                     /pseudo
     CDS             899..1318
                     /locus_tag="ACHOTW_005082"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT          405 a          341 c          317 g          321 t
ORIGIN      
        1 gtcagtctca ttcatctgga tgccagtgcc ggatgtctgc caggtttacc cacttttaat
       61 tggtaactag gaacaacatg acatcattaa ccccggaagc agcactggat attctgattg
      121 cgtggctgca ggacaatatc gacagcgaat ccggaattat cttcgacaac gatgaggata
      181 aaacggattc ggcagcattg ttgccctgta tcgaacaggt cagggaagat gtccgtaccc
      241 tgcgccaact gcagcttctg caacagaacc ggtgagtctc actcaccata cttcattcca
      301 ctgacgccag cctgaacagg gctggcgttt tcgtttataa accaaaagga aaaccgataa
      361 tgtcaggcat cataatctcc cgtccggaag tggacaccgg gcatacggac gttatctgct
      421 ccaccagtat ccgacacgtt gtgacggtac ggaatgcagc actgcaacaa accgaaacgc
      481 ttatccggca actggcagaa atctcagtcc tgacagcagc agacattggc ggcaaaaccg
      541 ccctggaccg ggcgatgaaa caggattttc actgcggctg ctggctgatg gagaaacctg
      601 aaaccgcaat gaaagccatc acccgcaacc tcgactgcga aatctggcgt gacctgatgc
      661 aacgctccgg gatgctgagt ctgatggatg cacagggcta ggatacatgg taccgctcac
      721 tggagtacga taatattccg gaaatcagcg aggcgaatat catgaatgca ttcaaacaac
      781 tgcaccagag taaggatgaa gtgtttgagc gtggggtgat caacgtcttc agagggccga
      841 acggaattac aaaaccaatt ccccctgtaa attcggcagt aaaattatcg tcaacaactt
      901 ggtgagatgg gacagatgga gatttcatct tatcagtgga atgcaggcag atcgcttagc
      961 tgacctggaa agaatgttgc atctgctcag cggtaaaccg ctccccaaca accgagggaa
     1021 tatcaccatt aatctggatg accacctaca gtccgttcag ggtaaagagc gctatgaaga
     1081 caagtaaata ctttaagaag ggcccgcaca catcacattc agaaagccag agctggttga
     1141 caggcttaat gatattattg ccagacacta cctaggaatg ttaccttcac aaaaatctct
     1201 aagaattgat tcaattgtgt attttagcct cattccctca tgtaaagtca tttgggggtt
     1261 attatggggg tcaatcattt atccaacacg cattaatcat ttaaaatcaa tgcattaata
     1321 atcatattcg actcctgtga tctccgccaa acttcctctt ctgaactctc ccgacgtcaa
     1381 caaa
//
LOCUS       DAZKIF010000345         1380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZKIF010000345 DAZKIF010000000
VERSION     DAZKIF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.349"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(92..634)
                     /gene="ybfC"
                     /locus_tag="ACHOTW_005083"
     CDS             complement(92..634)
                     /gene="ybfC"
                     /locus_tag="ACHOTW_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFP9846981.1"
                     /translation="MIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFPVLNNKAMWR
                     WYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGTLQELINTAE
                     KTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYARMTAHLPNK
                     DESYECLTKIQHELLRSEEK"
     gene            complement(659..>1380)
                     /locus_tag="ACHOTW_005084"
     CDS             complement(659..>1380)
                     /locus_tag="ACHOTW_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9846982.1"
                     /translation="EGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYY
                     NRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAR
                     PNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPN
                     LTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAG
                     IDDLGEILAKMKQRTSRGIRK"
BASE COUNT          346 a          312 c          264 g          458 t
ORIGIN      
        1 ccatttttcc aaatcgacct tctggtgtac ctggatatcc attaagaaca tgtttttccg
       61 aatggttaat ttattttctc aggaagatgt tttatttttc ttcactacga agcagttcat
      121 gctgtatttt tgttaaacat tcgtaagact catctttgtt cgggagatgt gctgtcattc
      181 gagcataagt tggcttctca tctttaaaat atttaaccat gattgaatta tcaaggactg
      241 ccaacaactg agctggatca ctgctctttt tttgatagat acccgctctt atataacttc
      301 taaaccgatc gccgagaaac gccgtttttt ctgctgtatt tatcaactcc tgcaacgttc
      361 ctttgatggc atgattttca ttaaggcgta gtgacccgac acgaattaag agtctcgccc
      421 cgcttggtgt gaatttatta tttttacatg tacccagttc tgtttgccaa taatactcac
      481 cgatgtcctc attctgatac catctccaca tggctttatt atttaagaca ggaaacgcgg
      541 aaaaaatagt acccttagcg aaaatagtaa aacaatcatg atctttagct tgtatttcgc
      601 aatcagctat aacaccaaaa ctaacaaata tcatcagcaa aaaaaaacaa aactcttttc
      661 attttcttat tcctctcgat gttctctgtt tcattttagc taaaatttca cccaaatcat
      721 ctatccctgc tgttgctggc ccatcgggtt tattaatgtc agggaataaa tagggttctg
      781 cacaagtata tcctggtggt aattgagttg ttggccaatt tgtctgtggc aggtaatcac
      841 ttgctgtctt tgtatccatc cctggatact ggggatcatc ctgtggcgta caagtcagat
      901 ttggttttac ccacttcctg caatacagtg tgtagtcgta agggcttaat gctctgaaga
      961 atgttttgaa aacattaggc gcactactgg cctggtcaat aggatatggc agtcttaatt
     1021 gtttcgctat gtctggaaat ttacttggat cgggcgtagg cggtttgttg ggtctcgctc
     1081 catatccgcc attatttaag tgtccatatt ttgatgccag tcccattgaa tctatatact
     1141 gaaccggatt caacggatac tgataaaaat tccatccccc cttcagtcca atcggatcct
     1201 gagtgatata cctcccctgc agcgggtcat aatagcggtg gcggttgtaa tacaggccgg
     1261 actcctcatc atactgctgc cccggcaggc ggataagctg ctgcagctga tgcgggttct
     1321 cttcattcag caggttgccc cactcatcgt attctgcgca ccattctgtt gccccttcct
//
LOCUS       DAZKIF010000346         1351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZKIF010000346 DAZKIF010000000
VERSION     DAZKIF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.350"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..139)
                     /locus_tag="ACHOTW_005085"
     CDS             complement(<1..139)
                     /locus_tag="ACHOTW_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846983.1"
                     /translation="MSYALIDNASLTAVERTLGDILVKNPDTINGDLVAFENLIQAIL
                     FY"
     gene            complement(326..502)
                     /locus_tag="ACHOTW_005086"
     CDS             complement(326..502)
                     /locus_tag="ACHOTW_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846984.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
     gene            805..>1351
                     /locus_tag="ACHOTW_005087"
     CDS             805..>1351
                     /locus_tag="ACHOTW_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9846985.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPE"
BASE COUNT          429 a          280 c          291 g          351 t
ORIGIN      
        1 cgtaaaaaag tatcgcttga ataagatttt caaaagcgac taaatctccg ttaatagtat
       61 ctggattctt aacaagaata tcacccagtg ttctttcgac tgctgttaac gaagcattat
      121 caatcaacgc ataagacatt aaactacctc cattagataa tatctaataa cattatattt
      181 caattaggtt taaaaattgt gatacttatg acagatttga gtgacatttt atcaatctac
      241 tgattttgat ggtgaaattt attagaaggt atgcgtagtg ctacgggaga aatgagaaac
      301 gcgcccagca atggcgcatc ctttcttaag caagttggct agtatctata gcatccatcc
      361 aatcaggagc aattcccaaa cgttccgcga actcttcatt ggtcacgtct tccattgaga
      421 gactagccag acaatcgtta atttcagaat agacggggga aagtaatagg tattcccgca
      481 actctttcag gtctttttcc atcttaacac tccttgttaa tagaccgtcg aacaatggta
      541 cagatttgta ccattccaag tactggcaag caaccccacc agcacaacaa attatctcat
      601 acggtaaaaa cggtgtcggg ctgtttcttt cggctagctc aatatatcac cttaaacacc
      661 agccgttatc attgtctcaa ttgggtatac cgtaatgaca aggtgataca aagtctcaat
      721 agggttgtat aggaacattt tgactacaca aactgcatta tgtatcattt gcacttatat
      781 tcacaaccct tttgagacaa tgcaatgaac gttagaattt actgccgagc atcaactgag
      841 gggcaacacg ctgatcgagc acttaccagc ctacgtgaat tttcaaaatc aaaaggctgg
      901 cagattgcag gagagtatat agaaaatgct agcggggcga agcttgagcg cgtagagctt
      961 atgcggctat tgtctgaagc ccagtccggc gatctgttgc tagtggaagc tatagatcgt
     1021 ctgagtcgcc tagaacatag tgcatgggtc gaactgaaag atacgctcaa ccgaaaaggg
     1081 ttaatcatcg tatcaatgga tttaccaacg agctggcaga tggtcgagat ggcaggcaat
     1141 gatctaacca gcggcatcct gagagccgta aatgccatgt tgattgatat ccttgccaca
     1201 atggcacggc aggattacga aacacgccgt aaacgccaac agcaaggcat tgaacgagct
     1261 aaaagcgaag gcatatacat tgggagggcg aaaaatcagg aagcccgaga gatcgttaga
     1321 gagatgttgg agcaaggagt aaaacctgaa c
//
LOCUS       DAZKIF010000347         1336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZKIF010000347 DAZKIF010000000
VERSION     DAZKIF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.351"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..609
                     /gene="nleB"
                     /locus_tag="ACHOTW_005088"
     CDS             <1..609
                     /gene="nleB"
                     /locus_tag="ACHOTW_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFP9846986.1"
                     /translation="DKKLSDIYLENIHKQESYPASERDNYLLGLLREELKNIPEGKDS
                     LIESYAEKREHTWFDFFRNLAMLKAGSLFTETGKTGCHNISPCSGCIYLDADMIITDK
                     LGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGL
                     GKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTCSSW"
     gene            658..1332
                     /gene="nleE"
                     /locus_tag="ACHOTW_005089"
     CDS             658..1332
                     /gene="nleE"
                     /locus_tag="ACHOTW_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFP9846987.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEIDSYMKYINN
                     KLSKIE"
BASE COUNT          465 a          196 c          271 g          404 t
ORIGIN      
        1 gataaaaaac tatctgatat ttaccttgaa aatatccata aacaagaatc ataccctgcc
       61 agtgagaggg ataattatct gttaggctta ttaagagaag agttaaaaaa tatcccagaa
      121 ggtaaggact ctttgattga gtcatatgca gaaaaaagag aacatacttg gtttgatttt
      181 ttcaggaatt tggccatgtt gaaggctgga agtttgttta cagagacggg aaaaactgga
      241 tgccataaca tatcgccctg tagcggatgt atatatcttg atgccgacat gattattacc
      301 gataaattag gagtcctgta tgctcctgat ggtatcgctg tgcatgtaga ttgtaatgat
      361 gagataaaaa gtcttgaaaa tggtgcgata gttgtcaatc gtagtaatca tccagcatta
      421 cttgcaggcc tcgatattat gaagagtaaa gttgacgctc atccatatta tgatggtcta
      481 ggaaagggta tcaagcggca ttttaactat tcatcgttac acgattataa tgctttttgt
      541 gattttattg aatttaagca tgaaaatatt ataccgaata ccagtatgta tacctgcagt
      601 tcatggtaag tcttccggtt gtggttgaaa aatatcgttc tttaggtata gattcagatg
      661 attaatcctg ttactaatac tcagggcgtg tcccctataa atactaaata tgctgaacat
      721 gtggtgaaaa atatttaccc ggaaattaaa catgattact ttaatgaatc acccaatata
      781 tatgataaga agtatatatc cggtataacc agaggagtag ctgaactaaa acaggaagaa
      841 tttgttaacg agaaagccag acggttttct tatatgaaga ctatgtattc tgtatgtcca
      901 gaagcgtttg aacctatttc cagaaatgaa gccagtacac cggaaggaag ctggctaaca
      961 gttatatccg gaaaacgccc aatggggcag ttttctgtag atagtttata caatcctgat
     1021 ttacatgcat tatgtgagct tccggacatt tgttgtaaga tcttccctaa agaaaataat
     1081 gattttttat acatagttgt tgtgtacaga aatgacagcc ctctaggaga acaacgggca
     1141 aatagattta tagaattata taatataaaa agagatatca tgcaggaatt aaattatgag
     1201 ttaccagagt taaaggcagt aaaatctgaa atgattatcg cacgtgaaat gggagaaatc
     1261 tttagctaca tgcctgggga aatagacagt tatatgaaat acataaataa taaactttct
     1321 aaaattgagt agagaa
//
LOCUS       DAZKIF010000348         1334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZKIF010000348 DAZKIF010000000
VERSION     DAZKIF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.352"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            317..>1334
                     /locus_tag="ACHOTW_005090"
     CDS             317..>1334
                     /locus_tag="ACHOTW_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846988.1"
                     /translation="MTIAKDSSTDEIIHGNDLRGMDDFYIKTTSFECPYEPCKIKATP
                     CSFTMKHVNQSYFRYGDKHKDGCGIHDPRYKNNHTSNDERKHNSPPAPVISLLKIDVK
                     PRGGVKNARNIKNENHKDEKKGNEHPVSSSSIKPVVDYYINNSNHNEQLSIPPYGTRS
                     YKDTFQLIFYKNNIRYYKPAIYYGVAQSNIRLHEDSDKHCITFLARDKKTQKPFTLEI
                     DVSDWNKSQKDVFWKEYEKQRKEADRYYKGLKDKRNAKKYLTVFFFGMPDENDKFLFK
                     TNHFKLVYVAFLGKFESSYNDSNYYIENDLSVSSLNEQPISLSDIDHKNHEYNIETSL
                     DFSSP"
BASE COUNT          486 a          204 c          225 g          419 t
ORIGIN      
        1 aatctaatta atgcttaaaa gcatcctcta agatgctttt actgttacca tgcatacata
       61 cccagaacct ttaacaaacg ataatagagg ggagttgtgt ataacaactc cctgacctta
      121 ctaaggttag cgatgttatt cagcatcatt ctgtcaaaga tttaatttct ttgaaaaata
      181 ctaattctac cccttaagtt aataacttct gcttgatcgt gtattttggt aagttggagt
      241 acaatttttt gattggatcg tcaataagaa aaagctagaa tccttagaag gacatagatt
      301 ttcatagggt taagatatga cgattgcgaa ggatagttcg actgatgaaa tcattcatgg
      361 aaatgatctc cgtggaatgg atgattttta cattaagaca acgagttttg aatgcccata
      421 cgagccttgt aaaattaaag ccacaccttg tagttttact atgaaacatg ttaatcaatc
      481 atatttcaga tatggcgata aacataagga tggatgtggt attcatgatc caagatataa
      541 aaacaatcat acttcaaatg acgaaagaaa acataattcg ccacctgctc cggttatatc
      601 acttttaaaa attgatgtga aaccaagagg tggtgttaaa aatgccagaa atattaaaaa
      661 tgaaaatcac aaggatgaaa aaaaaggtaa tgaacatcct gtatcttcat caagtattaa
      721 gccagtcgtt gactattaca taaataatag caatcataat gaacagctat caatccctcc
      781 atatggcacg cgatcttata aagatacatt tcaacttatt ttttacaaaa acaacatacg
      841 ttattacaaa cctgctattt attatggtgt agcccagtct aatattcgtc tgcatgaaga
      901 ttcagataaa cattgtatta ctttcctcgc aagggataag aaaacccaaa aaccatttac
      961 gcttgaaatt gatgtttctg actggaataa aagtcagaag gatgtgtttt ggaaggaata
     1021 tgaaaagcaa aggaaggaag ctgataggta ttataagggg cttaaagata aaaggaatgc
     1081 taaaaaatac ttgacggttt tcttttttgg gatgccggat gaaaatgata agtttctatt
     1141 taagacaaac cactttaaat tagtatatgt ggctttcttg ggtaaatttg aaagctcata
     1201 taatgatagt aattattaca ttgaaaatga tctgtctgta tcatcattga atgaacaacc
     1261 catttcatta tctgatatag accataaaaa tcatgaatat aatattgaaa cttcattaga
     1321 ttttagttct cctt
//
LOCUS       DAZKIF010000349         1333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZKIF010000349 DAZKIF010000000
VERSION     DAZKIF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.353"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1212
                     /locus_tag="ACHOTW_005091"
     CDS             <1..1212
                     /locus_tag="ACHOTW_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9846989.1"
                     /translation="RQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAAVASAPVIRVE
                     QKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAVGAGTTTDPQW
                     AGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSGGAAGWVGE
                     GKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVARLDTD
                     FVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQPTGAVWLMSST
                     NALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGV
                     AVDMSREASLEMQSEPGGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVI
                     TGVNYGSASGG"
     gene            1257..>1333
                     /locus_tag="ACHOTW_005092"
     CDS             1257..>1333
                     /locus_tag="ACHOTW_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9846990.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCA"
BASE COUNT          253 a          359 c          453 g          268 t
ORIGIN      
        1 cgtcaggtgg atgcgcacct gaagcgcctg cgtgaactgg aagccggtaa ggccgccacg
       61 gcgcagccgg tgaaacaggc cggtaacggg aatgtggccg cggtggcttc tgcgccggtg
      121 atccgtgtgg agcagaaact ggataagggg attggcttcg cccgctttgc caaatcgctg
      181 gctgcggcta aaggcgtccg atctgaagcc ctggaagtgg cccgtcgtca gtatccggat
      241 gacagtcgtc tgcatcatgt cctgaaatcg gcagtgggcg cggggaccac cacggatccg
      301 cagtgggcag gcagcctgtc tgaatatcag gaatacgcac aggactttat tgattacctg
      361 cgtccgcaga ccattatcgg gcgatttggt cagggcggga tccctgcact tcgtcaggtg
      421 ccattcaata tccgtgtgca cgcccaggtg tccggcggtg ctgccggctg ggtgggtgag
      481 ggtaaggcaa aacccctgac gaagtttgat tttgaatcca tcaccttcag tcatgcgaag
      541 gtgtcggcca ttgcggtact gacggaagaa ttgatccgtt tttccagtcc ggctgctgat
      601 gcactggtcc gtaatgcgct ggcggaagcg gtggtggcgc gtctggatac agactttgtg
      661 gacccgaaaa aagccgcagt ggcagatgtc tccccggcgt ccatcaccca tgatgtgaag
      721 ggcacggcat caaccggtaa cccggatgcg gatgccgagg ctgcgtttgg acagtttgtg
      781 gcagcaaacc tgcagcccac cggtgcggtc tggctgatgt ccagcaccaa tgccctggca
      841 ctgtccatgc gtaaaaatgc gctgggtcag aaggaatacc cggacatgac cctgctgggt
      901 ggctccttcc aggggctgcc ggtgattgtc tcccagtacg tgggtgacca gctggtgctg
      961 gtgaatgccc cggatattta tctggcggat gacggcggcg tggcagtgga tatgtcccgc
     1021 gaggcatcac tggaaatgca gtctgagccg ggcggcgaca gtaccacgcc gtccccggtg
     1081 gagctggttt ccatgttcca gacaggcagc gtggccatcc gtgcggagcg ctggatcaac
     1141 tggcgtcgtc gccgtactgc ggcggtggcg gtgatcaccg gagtgaacta cggcagtgcg
     1201 tccggcggct gagtctgata aggaggacgg gaggcgtgcg cctcccgtaa caggttatgg
     1261 caaagatccg atatctgcag ggcacgcatg atgcccgggc cggggatatc cgtgatgtgg
     1321 cacagccgtg tgc
//
LOCUS       DAZKIF010000350         1330 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZKIF010000350 DAZKIF010000000
VERSION     DAZKIF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.354"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            23..>1330
                     /gene="nleA"
                     /locus_tag="ACHOTW_005093"
     CDS             23..>1330
                     /gene="nleA"
                     /locus_tag="ACHOTW_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFP9846991.1"
                     /translation="MNIQPTIQSGITSQNNQHHQTEQIPSTQIPQSELPLGCQAGFVV
                     NIPDDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNV
                     TQMALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASTGTSATTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVK
                     TENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDL
                     DNPNTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPG
                     LKPDTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGAD
                     IRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFDII"
BASE COUNT          427 a          278 c          277 g          348 t
ORIGIN      
        1 aaacatgtaa taaggattta ttatgaacat tcaaccgacc atacaatctg gaatcacctc
       61 acaaaacaat caacatcatc aaacagaaca aataccctct acacaaatac cgcaatccga
      121 attacctcta ggatgccaag ctggatttgt tgttaatatt ccagatgata tacagcaaca
      181 tgcaccggaa tgcggtgaaa caacagctct actgagcttg ataaaagata aaggtctgct
      241 ctcagggcta gacgaatata tagctcctca ccttgaagaa ggatccatag gaaaaaaaac
      301 attggatatg tttggtttat tcaatgttac ccaaatggca ttagagatac ctagttccgt
      361 ttcaggcatc tctggtaaat atggtgtcca gctaaacatt gtaaaaccag atattcatcc
      421 tacatcaggt aattattttt tacagatatt ccctctgcat gatgaaatag gttttaattt
      481 taaagacctt cctggcccgt taaaaaatgc attaagcaac agtaatatat caaccactgc
      541 agtgtcgact attgcatcga ctggaacatc agccactact tcgacggtaa ccaccgagcc
      601 aaaagaccca ataccatggt ttggattaac agctcaagtg gttcgtaatc atggtgtaga
      661 acttcctata gtcaaaactg aaaatggatg gaagcttgtt ggagaaacac cacttactcc
      721 tgatgggccg aaagcaaatt acacggagga gtgggttatc agaccgggag aagcagattt
      781 taaatatggt gcatctccat tacaggcaac tctagggctg gagtttggcg cacatttcaa
      841 gtgggattta gataacccta atactaaata tgccgttctt accaatgctg ccgcaaatgc
      901 gcttggtgct ttagggggat ttgcagtatc cagatttgct agtacagatc caatgttaag
      961 tcctcatatc ggtgcaatgg ttgggcaagc agcagggcat gccatacagt ataatacccc
     1021 tggattaaag ccagacacta ttttatggtg ggctggtgcg acactggggg ctgccgattt
     1081 aaacaaggcc gagtttgaag tagctagatt cactgactat cctcgtatat ggtggcacgc
     1141 aagagaagga gctattttcc ccaataaagc agatattgaa catgccacag gtgctgatat
     1201 acgcgcaatg gaagaaggta tccctgttgg acagcggcat ccaaatccag aggatgtggt
     1261 aatcgatatc gaaagcaatg gcttaccaca tcataatcca tcaaatcatg ttgatatctt
     1321 tgatataatc
//
LOCUS       DAZKIF010000351         1330 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZKIF010000351 DAZKIF010000000
VERSION     DAZKIF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.355"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..>1330
                     /locus_tag="ACHOTW_005094"
     CDS             2..>1330
                     /locus_tag="ACHOTW_005094"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9846992.1"
                     /translation="MLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQIIND
                     ELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVAD
                     LWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQI
                     CWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQP
                     LHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLA
                     PLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREG
                     YTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTLYDLS
                     ERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKY"
BASE COUNT          296 a          356 c          369 g          309 t
ORIGIN      
        1 gctgttaacg gatttccgct cagaactact cgattcacgt tttggcgcaa aggccatttc
       61 tactatcgcg gagtcaaaac gatttccgct gcacgaaatg cgcgatgatg tcgcattcca
      121 gattatcaat gatgaattat atcttgatgg caacgctcgt cagaacctgg ccactttctg
      181 ccagacctgg gacgacgaaa acgtccataa attgatggat ttgtcgatca ataaaaactg
      241 gatcgacaaa gaagaatatc cgcaatccgc agccatcgac ctgcgttgcg taaacatggt
      301 tgccgatctg tggcatgcgc ctgcgccgaa aaatggtcag gccgttggca ccaacaccat
      361 tggttcttcc gaggcttgta tgcttggcgg gatggcgatg aaatggcgtt ggcgcaagcg
      421 tatggaagct gcaggcaaac caacggataa accaaacctg gtgtgcggtc cggtgcaaat
      481 ctgctggcat aaattcgctc gctactggga tgtggagctg cgcgagatcc ctatgcgccc
      541 cggtcagttg tttatggacc cgaaacgcat gattgaagcc tgcgacgaaa acaccatcgg
      601 cgtggtgccg actttcggcg tgacctacac cggtaactat gagttcccgc aaccgctgca
      661 cgatgcactg gataaattcc aggccgacac cggtatcgac atcgacatgc acatcgacgc
      721 tgccagcggt ggcttcctgg caccgttcgt cgccccggat atcgtctggg acttccgcct
      781 gccgcgtgtg aaatcgatca gtgcttcagg ccataaattc ggtctggctc cgctgggctg
      841 cggctgggtg atctggcgtg atgaagaagc gctgccgcag gaactggtgt tcaacgttga
      901 ctacctcggt ggtcagattg gtactttcgc catcaacttc tcccgcccgg cgggtcaggt
      961 gattgcgcag tattatgaat tcctgcgcct cggtcgtgaa ggctatacca aagtacagaa
     1021 cgcctcttac caggttgccg cttatctggc ggatgaaatc gccaaactgg ggccgtatga
     1081 gttcatctgt acgggtcgtc cggacgaagg catcccggcg gtttgcttca aactgaaaga
     1141 gggtgaagat ccgggataca ccctgtacga cctctctgaa cgtctgcgtc tgcgcggctg
     1201 gcaggttccg gccttcactc tcggcggtga agccaccgac atcgtggtga tgcgcattat
     1261 gtgtcgtcgc ggcttcgaaa tggactttgc tgaactgttg ctggaagact acaaagcctc
     1321 cctgaaatat
//
LOCUS       DAZKIF010000352         1307 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZKIF010000352 DAZKIF010000000
VERSION     DAZKIF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.356"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1307
                     /locus_tag="ACHOTW_005095"
     CDS             <1..>1307
                     /locus_tag="ACHOTW_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9846993.1"
                     /translation="KGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLVETTSKGDRNPTSVRLLIQLERGGKWMTEKDVTINGKTTSQ
                     FLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAW
                     DVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKD
                     RHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFG"
BASE COUNT          297 a          344 c          433 g          233 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacgcctcga gaggcgaagg ataatctcaa atccacgcag
       61 atgatgagtg tgattgatgc gattggtgag ggaccgatag aaggcccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggtaatcc cgtgatacac
      181 ggtgtgactg cggtctggcg tgccggggag caggagcaga caccaccgga aggctttgag
      241 tcctccggag ctgaaaccgg actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacgt ccgcgaacat tgaccgcctg cgggttacct tcggggtgca gtcactggtg
      361 gagaccacct caaagggtga ccgtaacccg acgtctgtcc gcctgctgat tcagcttgag
      421 cgtggtggta aatggatgac ggaaaaggat gtcaccatta acggcaagac cacctcgcag
      481 ttcctggcgt cggtgattct ggataatctg ccgccccggc cctttaacat ccggatggtc
      541 agggagacgg cggacagcac cacggaccag ctgcagaata agacgctctg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgattgtggg gctgcaggtg
      661 gatgcggagc agtttggcgg tcagcagatg acggtgaact accatatccg cggtcgcatt
      721 attcaggtgc cgtcaaacta tgacccggaa aaacgcacgt acagcggcat ctgggacggc
      781 agcctgaaac cggcatacag caacaacccg gcctggtgtc tgtgggacat gctgactcac
      841 ccgcgctacg ggatgggaaa acgtctgggg gcggcggatg tggacaaatg ggcgctgtat
      901 gccattgcgc agtactgcga ccagacggtg ccggatggtt tcgggggcac agagccgcgg
      961 atgaccttta atgcatacct gtcacaacag cgtaaggcgt gggacgtcct cagtgatttc
     1021 tgctcggcga tgcgctgtat gccggtatgg aacggccaga cgctgacgtt cgttcaggac
     1081 cgcccgtcgg atgtggtgtg gccgtacacc aacagcgatg tggtggtgga tgataacggc
     1141 gtggggtttc gctacagctt cagcgccctg aaggaccgcc acacggcggt ggaggtgaat
     1201 tacaccgacc cgcagaacgg ctggcagacc tccacggaac tggtggaaga cccggaagcc
     1261 atactgcgct acgggcgcaa cctgctgaag atggatgcgt tcggctg
//
LOCUS       DAZKIF010000353         1279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZKIF010000353 DAZKIF010000000
VERSION     DAZKIF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.357"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..327
                     /locus_tag="ACHOTW_005096"
     CDS             <1..327
                     /locus_tag="ACHOTW_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9846994.1"
                     /translation="AGATMALWGAALSAGGLTATTMLFSLGASMILGGVAQMLAPKAK
                     TPDYRATDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKV
                     VVIGRG"
     gene            568..>1279
                     /locus_tag="ACHOTW_005097"
     CDS             568..>1279
                     /locus_tag="ACHOTW_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9846995.1"
                     /translation="MGKGGGKGHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYH"
BASE COUNT          322 a          306 c          408 g          243 t
ORIGIN      
        1 gccggcgcaa cgatggcgtt gtggggcgca gccctgagtg ccggagggct gactgccacc
       61 acgatgctgt tctcactggg tgccagcatg atactgggcg gtgtggccca gatgctggca
      121 ccgaaggcaa aaacaccgga ttaccgcgca acggataacg gtaaacagaa cacgtatttt
      181 tcgtcactgg ataacatgat tgcccagggg aacccgatgc cggtgcctta tggtgaaatg
      241 ctggttggtt cacgacggat atcccaggac atcagcaccc gtgatgaggg cggagacggg
      301 aaagtggtgg ttatcgggcg gggatgaaaa taaaaaaatc ccgcagagtt agcggagctg
      361 cgggagagaa cgatgaagat taacgttatg gagttatttt tcaggcatca aaaaagtaat
      421 gcagcgtcat tattgcggct acaggcaatt gccggaaatg tgaagagttt cagaaatttt
      481 attccgtcat gacacaggca ccctccgggg tgcctgttgt tttctggcat aaacagattc
      541 agacatcaga caggagaggg ggacagagtg ggtaaagggg gcggcaaggg gcacacgccg
      601 gtagaggcaa aggacaatct taagtccacg cagatgatga gcgtgattga cgccattggt
      661 gaagggccga ttgaaggtcc ggtgaagggg ctgcagagta ttctggtgaa caaaaccccg
      721 ctgacggaca cggacggtaa tcccgtgata cacggtgtga ctgcggtctg gcgtgccggg
      781 gagcaggagc agacaccacc ggaaggcttt gagtcctccg gagctgaaac cgcactgggc
      841 gtggaagtga cgaaggcaaa gccggtgacg cgcaccatta cgtccgcgaa cattgaccgc
      901 ctgcgggtca ccttcggggt gcagtcactg ttggagacca cctcaaaggg cgaccgtaat
      961 cactcttctg tccgactgct gattcagttg cagcgtaacg gtaactgggt gacggaaaag
     1021 gatgtcacca ttaacggcaa gaccacctcg cagttcctgg cgtcggtgat tctggataat
     1081 ctgccgcccc ggccctttaa catccggatg gtcagggaga cggcggacag caccacggac
     1141 cagctgcaga acagaacgct gtggtcgtca tacaccgaaa tcatcgatgt gaaacagtgc
     1201 tacccgaaca cggccattgt ggggctgcag gtggatgcgg agcagtttgg cggtcagcag
     1261 atgacggtga actaccata
//
LOCUS       DAZKIF010000354         1275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZKIF010000354 DAZKIF010000000
VERSION     DAZKIF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.358"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..394)
                     /locus_tag="ACHOTW_005098"
     CDS             complement(<1..394)
                     /locus_tag="ACHOTW_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFP9846996.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKN"
     gene            complement(1029..>1275)
                     /locus_tag="ACHOTW_005099"
     CDS             complement(1029..>1275)
                     /locus_tag="ACHOTW_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP9846997.1"
                     /translation="NIYKQLRYLEKSASQGNVTAQFNYGVFLSDTNPTLSEYYNLNRA
                     IYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          371 a          258 c          213 g          433 t
ORIGIN      
        1 tattcttatc ttttatttta tttactgttt caatgcaatc agcggcaagt tggagtggat
       61 ttagattttt cagatatggt cttatgatct ttcccttact taataaatag catgtatagg
      121 attcggaaag tccagcatct attttgtatc tcattatatt agataaggat gtgagaaatt
      181 ccttgttatc attcgcagcc atatatgcca gtccgagttc agcctctgcg gccttacagg
      241 caaaatagct tagtgaacac gcttttcgtc cctctttagt actggctgat attagtgccc
      301 ataatattgc tttgtctgcc aaatctaact ctctctgaaa ttgtcttgca gtaactgcac
      361 tcgcagaaat agagaaaaaa atacaattgt taataacctt gccatatttt ttagtccgta
      421 actgttaatg tgctatctgg ctctgaaaat attaactcat ttaatttttg catgtcatta
      481 gataaaccag ttatacaacc ctcggaacag aatcgaggtc ctaaagacgg accaaaagcg
      541 tttaaacaac tatgtattga aaatccattg cgattataag ttcctgtatt aactgacggt
      601 actaatcgta tgccgtcagg ttttctattg cttgctcccg ggtcattaac attccaactc
      661 cataggcctt gagatattgg ccatctattt ataaatgtgt acaacgggga ttatttttac
      721 attgcatatt attaccagaa gcgactggaa tactaagcac atcataaaaa ttatcatcac
      781 atggtatatc tgaaacacac gaaagcatac ctaaagttat gctgtataaa caggtggcta
      841 atcccaaggg gatcgattcc agaaatcggg ttcaacggat actgataaaa attccaccta
      901 ccttccagcc caatcggagc ctgagtgtta taccgcccct gctgcggtta atgttaccga
      961 taatagttat agtacagccc tgattcatca tcatactact gctgcggcag tctgatgaac
     1021 tattgctttt acggattctc cttattcttc cttctatcca ttcgctttag ctttttgagc
     1081 tcttgaagtt tgctctttgc atcaatatta ccgttattta cagcgaattc catccaataa
     1141 atagctctat tcaaattata atattctgat aatgttggat ttgtatctga taaaaagacc
     1201 ccataattaa actgcgctgt aacattcccc tgagatgctg atttttctaa gtatcgcaat
     1261 tgtttgtaaa tgtta
//
LOCUS       DAZKIF010000355         1274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZKIF010000355 DAZKIF010000000
VERSION     DAZKIF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.359"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1048..1236
                     /locus_tag="ACHOTW_005100"
                     /pseudo
     CDS             1048..1236
                     /locus_tag="ACHOTW_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          385 a          231 c          287 g          371 t
ORIGIN      
        1 tttttgatag atattttacg actgaacata tccgtcgttt cctctgactc gtgtcagtcg
       61 gtctctggtt gtatccacgg accgcactct tgacataaga gtgcggtccg tggaaaacgg
      121 agttgaaaaa atggtgtctg aaagaaggta ccatcattgt gatgacaaat actttggcgt
      181 ggataatgta attaattgct gaaattgacc gtatatgcgg taagactcat atcagtatca
      241 ggtccggtta attgctgata ctaatataaa ctttttacaa aaggatgaat gcagaaggtt
      301 actctaatgg ccagagtgat gacaatgatg gtcagacgtt gacctgcaga aactgcattt
      361 taattggtct ataaagtctg caagaggaat cagggtcaga tcagtgagtc ttatcgcggc
      421 ccgaagctat gactgaaaga aggctggata cagaattgca acaatttggc aatgtcagaa
      481 cacagaagct ttgcaagcag gtgaaaggtt atgtctagca aaaggagtat atcagaatat
      541 atctactgat gtacaaaaca caacaatagc ttcatgatat ataaatacgt gatgacgagt
      601 ggtactccgc atggttattt aacttctaag caccactaat gatcctgagc aacttcgtat
      661 ttgggccatt accatggagt agaaagctgt gtctgagaat gcagaatttg agaaaagaat
      721 tcgctttctt ggagagattc tgaaaatggc acgctaacaa cgttttgtta aaaaatgtga
      781 attacttgcc ggggctgcat tttctattcg aggagaatgt tgacgtagat attacagaaa
      841 tctcagcgca tcaggataaa ctgctacctt aggtcaacca ccaaaagaaa atacaatacg
      901 tccagtttgt atgcctttaa taacacatct tctaaatgtt gaagaattat cccgcctgct
      961 gaagaacgtt gctctgagtg taataaacct ttttattctt gatgatgtca gcgagaagat
     1021 ggaatatatc cccgactggg tcgtggttac ccggttaaac atctggtggt gctaataaat
     1081 ctggcaagtc acattctgtt gaatgctgtg acagtgcctt accgagagag cgaaattgtg
     1141 gtgttctagt gcatagtcgc caccattccg gataattcta ttacgttgtt ttacaagttc
     1201 ttttacagtg cagatctgtc gctgacgttg aatcaatagc ctggatttca gaacacaatg
     1261 cccgtttgct ggtt
//
LOCUS       DAZKIF010000356         1273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZKIF010000356 DAZKIF010000000
VERSION     DAZKIF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.360"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..75
                     /locus_tag="ACHOTW_005101"
     CDS             <1..75
                     /locus_tag="ACHOTW_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9846998.1"
                     /translation="RISQDISTRDEGGGGTVVVVGRQG"
     gene            417..>1273
                     /locus_tag="ACHOTW_005102"
     CDS             417..>1273
                     /locus_tag="ACHOTW_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9846999.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPERPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYG"
BASE COUNT          332 a          304 c          383 g          254 t
ORIGIN      
        1 cgtatatccc aggacatcag cacccgtgat gaaggcgggg gcggaacggt cgtggttgtc
       61 gggcgacagg gataaaacat aaaaaaatcc cgcagtgatc gcggagctgc ggggacagac
      121 aaatgaagat caatgttaag gagttgtttt tgttactcgg gcaaaaaaac actaacgcag
      181 cgaaattata agcgccacag tcagtgtgtg aaaatgtgaa gatattcaga atttttatgc
      241 cattaccggt tttaaccaac aggattatcg gtgggcatga aagaaaaccc cggtatctgc
      301 tgataccggg gttttcttta gcatggcaga aatgtgtttc atgcttttcg ggcgaaggat
      361 atccgacttc tgtacggaat ggcaagtggc ggttaattta ttcaggggaa ggctgtatgg
      421 gaaaaggtgg cggtaaggca cacacgcctc gtgaggcgaa ggataatctc aaatccacgc
      481 agatgatgag tgtgattgat gcgattggtg agggaccgat agaaggtccg gtgaagggac
      541 tgcagagtat tctggtgaac aaaaccccac tgacggacac ggacggcaat cccgtgatac
      601 acggtgtgac cgcggtctgg cgtgccgggg agcaggagca gacaccaccg gaaggctttg
      661 agtcctccgg agctgaaacc ggactgggcg tggaagtgac gaaggcaaaa ccggtgacgc
      721 gcaccattac gtccgcgaac attgaccgcc tgcgggtcac cttcggggtg cagtcactgt
      781 tggagaccac ctcaaagggc gaccgtaatc actcttctgt ccgactgctg attcagttgc
      841 agcgtaacgg taactgggtg acggaaaagg atgtcaccat taacggcaag accacctcgc
      901 agtttctggc gtcggtgatt ctggataatc tgcctgagcg gccctttaac atccggatgg
      961 tccgggagac agcggacagc acctcggacc agctgcagaa taagacgctc tggtcgtcat
     1021 acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgattgtg gggctgcagg
     1081 tggatgcgga gcagtttggc ggtcagcaga tgacggtgaa ctaccatatc cgcggtcgca
     1141 tcatccaggt accgtcaaac tatgacccgg aaaaacgcac gtacagcggc atctgggacg
     1201 gcagcctgaa accggcatac agcaacaacc ctgcctggtg cctgtgggac atgctgaccc
     1261 acccgcgcta cgg
//
LOCUS       DAZKIF010000357         1273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZKIF010000357 DAZKIF010000000
VERSION     DAZKIF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.361"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1251
                     /locus_tag="ACHOTW_005103"
     CDS             <1..1251
                     /locus_tag="ACHOTW_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847000.1"
                     /translation="YKKEYRDKRIAKFDFIKFVSESDFQLSELDQLAQVESRQITSEI
                     RGGEFVDDDFRQLVEMLKLNMICTWDLRSSVYYLTMKMLGQPGTPEYAKYSELSASIF
                     NELSDASDTKGYWSTDVKLVSSSGHEYTEREFELEREKSTRGKGGMTRSLEMFIASLN
                     WLAYKSIYYSVIAKHFKADSFIHPIRHAYQLHWMKKTGAFGHDYTARLVQSLSDKIST
                     ARSEIVDHGRTSTISLDLPIFSAWLANETGNVSQVINSALELRTHDHFRTCRDVIREI
                     HVTYDESGISAGNKKVTKLLSDLDKISGDIKRSYGVPSNQGIQGSFLIKCINSLTGLV
                     GIPGLPDKEFALSTPEFMKSKQHKAFSTVFKDVTNELTSIERLGGLRDKLASNFTIDD
                     SYYTEPKTEDPRFRYVSSNWKQPM"
BASE COUNT          423 a          195 c          263 g          392 t
ORIGIN      
        1 tataaaaaag aatatagaga taaacgcatt gctaaatttg actttattaa atttgtatct
       61 gaaagtgatt ttcaactcag tgaacttgat cagttagcac aagttgaatc tcggcaaatt
      121 acttccgaaa ttagaggtgg ggagtttgtt gacgatgatt ttaggcaact tgtagagatg
      181 cttaagctga atatgatttg cacatgggat cttcggtcta gcgtgtatta cttaacaatg
      241 aaaatgcttg gtcagcccgg tactcctgaa tatgcgaaat atagtgaact aagtgcatcg
      301 atttttaatg aattatcaga tgcttctgat acgaaagggt actggtcaac cgatgtgaag
      361 ctagtgagtt catctggaca tgaatatact gagcgtgaat ttgaactaga aagggaaaaa
      421 tctactcgcg gtaaaggagg tatgactcgt tcacttgaaa tgtttatcgc atcgctaaat
      481 tggttggcat ataaatctat ttattacagc gtcattgcta agcattttaa agcagactca
      541 tttattcatc caataaggca tgcatatcaa ttgcattgga tgaaaaaaac aggtgctttt
      601 ggtcatgatt atactgcaag gttggtgcaa tcgttgtctg ataaaatcag tactgcaaga
      661 tctgaaattg tagatcatgg acgcacatct actatttcat tagatttacc aatattttct
      721 gcatggctag caaacgaaac aggaaatgtc tctcaagtta tcaattcagc tttagagctt
      781 agaacacatg atcattttcg tacgtgtcga gatgtaatcc gagagattca tgtgacctac
      841 gatgaatcgg ggataagtgc aggaaataaa aaagtaacaa aattactaag tgatttagat
      901 aaaatatctg gagatattaa acgaagctac ggtgttccat caaatcaagg tattcaaggc
      961 agttttttga taaaatgtat aaattcattg acaggactcg tgggtattcc aggtttgcct
     1021 gataaagagt ttgcactgag cactcctgaa tttatgaagt ctaaacaaca taaagcattt
     1081 tctaccgttt tcaaggatgt tactaacgaa ctaacaagca ttgaacgttt aggtgggctt
     1141 cgggataaat tagcgtcgaa ctttactatt gatgattctt attataccga gccaaaaaca
     1201 gaggatccga gattccgtta cgtaagtagt aattggaagc agccaatgta gatatagtta
     1261 aatgtataat tat
//
LOCUS       DAZKIF010000358         1265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZKIF010000358 DAZKIF010000000
VERSION     DAZKIF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.362"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..241
                     /locus_tag="ACHOTW_005104"
     CDS             <1..241
                     /locus_tag="ACHOTW_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9847001.1"
                     /translation="NNTTDFRPDPEDIFKCGQTLECGDPRKNIRQKYGHHGLGVNQNQ
                     QFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPH"
     gene            503..>1265
                     /locus_tag="ACHOTW_005105"
     CDS             503..>1265
                     /locus_tag="ACHOTW_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9847002.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVLEKVDDWQIERFQQSIQDDKISFTIQTDYSEKYSMLSGMRAHILRR
                     NNHYQFIVTINSKNYGCPLDNTDINWCGIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQV"
BASE COUNT          419 a          206 c          252 g          388 t
ORIGIN      
        1 aaataacact actgatttca gaccagatcc tgaagatatc ttcaaatgtg gacagacgct
       61 cgaatgtggc gacccccgca aaaacattag acaaaaatat ggccatcatg ggttaggggt
      121 aaatcagaat caacaatttg ataatggtgt tatggggcta aattccagga tggcagtgtt
      181 tgatgaaaat aaaaaaataa taggttcttc ctcaggacta agttgttttc aaccacatta
      241 aaactctatg agtgctgtgg tatatctttt taatcctgcc agtatttcat agtttttctt
      301 aatcgattgt agaggcatgt actgatgacg cctctacaat ctttagggac tttctctact
      361 atgagataag aaatttcata ttatgcttta aggctgaatg atttgtgcaa aatataatta
      421 tttttccatc gaacatgagg tgtatgtcat aagattaaat gataactatg tattcctcgc
      481 ggtgaattat atgaggtaaa agatgaaaat tggaactgtg gcaggtacta acgacagtac
      541 aacgacaatc gcaacaaatg atatggtgca agagcatgtt actaacttca ctaaagaact
      601 gtttgggtat attgcaaatg gtataggtga tgatatatca agtattgcca ggacaatgct
      661 cggtgaggtt ctggaaaaag tagatgattg gcaaatagaa cggttccaac aatctattca
      721 agatgacaaa atttcattta ccatacaaac ggattattca gaaaaatata gtatgctatc
      781 gggcatgcgt gctcatattc tacgtaggaa taaccattat cagtttattg tgacgattaa
      841 tagcaaaaat tacggttgcc ctcttgataa taccgatatt aattggtgcg gcattgttta
      901 tttacttaat aatatgacag taaatgataa tgctaatgat gttgcagtta ctgagtcgta
      961 caaacccatc tggaactgga aaatatcgca atacaatgta tctgatatta aattcgaaac
     1021 aattataaaa ccccagttcg ctgacagaat ctatttctcc aattgttcac ctgttgatcc
     1081 cacaagtacc agaccaacct attttggtga taccgatggc tcagttggtg cagttttgta
     1141 cgcgcttttt gcaacgggac atttgggaat catggctgaa ggagaaaatt tccttagcca
     1201 gttgcttaat atagaagatg aagttctcaa tgtattgcta agggagaact ttaatgagca
     1261 agttg
//
LOCUS       DAZKIF010000359         1240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZKIF010000359 DAZKIF010000000
VERSION     DAZKIF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.363"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..81
                     /locus_tag="ACHOTW_005106"
     CDS             <1..81
                     /locus_tag="ACHOTW_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847003.1"
                     /translation="VRAQPVSGGRLGVKIYPIMLEGQVKK"
     gene            78..899
                     /locus_tag="ACHOTW_005107"
     CDS             78..899
                     /locus_tag="ACHOTW_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9847004.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDFRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1137..1202
                     /locus_tag="ACHOTW_005108"
                     /pseudo
     CDS             1137..1202
                     /locus_tag="ACHOTW_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          336 a          240 c          354 g          310 t
ORIGIN      
        1 gttcgtgctc agccagtatc tggtggacgt ctgggggtga agatttaccc cataatgctt
       61 gaagggcagg tcaaaaaatg aaactggaag atttaccgaa atactactcc ccaaaatccc
      121 ccggcctgac tgatgcatcg gcctcaacgt cgaaagatac gctgagtatc actgatgtga
      181 tggccgcgca gggcatgaca cagaattggg ctgagatggg gttttctgcg ttccttggga
      241 aaatgggcat tagtatgaat gacagagagc gggcaacaga attgctgaca gaatatgcac
      301 tcagtcggtg tgatcgcgtg gcggcgttaa gaaaactccc ggcagaaata aaaccggcag
      361 tgatgcgtat tatggcttcg tatgcgtttg aagattatgc ccgtagcgcg gcgagcaaaa
      421 aacagtgccc ctgttgtcac ggaaaaaaat ttattgaaag cgaggttttt acaaacaaga
      481 tccagtatcc ggatggtaag ccgccagtgt gggcaaagtg cacaaaaggc gtgtatccgt
      541 cttactggga ggaatggaaa aaagtcaggg aggtggtaaa agttgcctgt ccggagtgtg
      601 gagggaaggg ggaggtttcc accgcctgta aagattttcg tgggcgcggt gttgccattc
      661 atcgtgaaga gtcggtaaaa cgtggtatgc ctgttatcag agactgccag cgttgtggtg
      721 gtcgtggcta tgaaagatta ccttcaacgg aggcatttaa tgccatatgt aatgtaaccg
      781 atgccatatc tcttgataca tggaaaaaaa cagttaaacg tttttacgat acgctggtgg
      841 tgcagtttga tattgaagaa gcatgggcag aacaacaact gaaaaaggtg accagatagc
      901 tttgttgatt tttcccgaat ctgtggtaaa tttgccctaa cgatgggcgt tttatgcctg
      961 acgttagaag attttttaca cccgtcgcca ggcgggtttt tttatgactg aaatcacgcc
     1021 agtacagtaa acgcgctggt ggttgtgaat accggtcttt ctgcttgctg gctttttcga
     1081 caagagttat tggtgtgtca cgttaaccgg aaaaaggaaa gtttgagaaa cgcgatctgg
     1141 cacaggcggt gattaacgcc gcatacctgg tggcctgtgc agatggtgaa tgtgaggctt
     1201 cctagaaagc gaagatcgaa caggtactgc gtaatcagcc
//
LOCUS       DAZKIF010000360         1240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZKIF010000360 DAZKIF010000000
VERSION     DAZKIF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.364"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..400)
                     /locus_tag="ACHOTW_005109"
     CDS             complement(<1..400)
                     /locus_tag="ACHOTW_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9847005.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHK
                     FRCVPHLTGRLFEHGVTDCYTLFRDAYHLAG"
     gene            complement(411..1109)
                     /locus_tag="ACHOTW_005110"
     CDS             complement(411..1109)
                     /locus_tag="ACHOTW_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9847006.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLREIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(1109..>1240)
                     /locus_tag="ACHOTW_005111"
     CDS             complement(1109..>1240)
                     /locus_tag="ACHOTW_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9847007.1"
                     /translation="GWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSAEFKQVVN"
BASE COUNT          271 a          437 c          287 g          245 t
ORIGIN      
        1 ttcccgccag atggtatgca tcccggaaca gcgtgtaaca gtccgtcacc ccgtgctcaa
       61 agagccgtcc tgtcagatgt ggcacacagc ggaatttatg aatttccccc cggcagacca
      121 gccaccagga cagtgcactt tttatctgca gccgccggtc ggcctcgctc agccagggca
      181 gaccaccggg atgactgtgg accagtgcca caatctcccc ctgcatctct gcccgcagcc
      241 agtcttccgg tgcaatacga aaatacgcct ccggctctgc agagatattc acacaaggga
      301 tataccgctc cccctccggc gttctcacca cgaagccgca cgactccgca ggcgcacacc
      361 gccgggcatg cgccagaatc gctgattcag tctgtgtcat aaaccgggat ttactgcgaa
      421 agtttattaa tggaaaggaa accgccaaaa ttagccacca tgccgcgcat ctcacacccg
      481 cgcatgcact tgctgcatct gtccttacgg atatcggtgg tgggtttatc gaactcatcc
      541 gccacagccc cgcccgtgta accacactca tcagagcggt aggtccacat acaggtattc
      601 gccagcataa tgcgaccggg aaacagcgct ccgtccgtct ccgtcggtgt ggccagcaca
      661 aacgaggctg tcatggccgt cagctctgac atctgctcca ccacccagcg gtcgctcagc
      721 tcctgctccg ggtccgcttc cggattgccc gccacaaaat tcaccgcatc cagaaaacgg
      781 gcatacaccc ggcggcggac caccgtggcc cccaccaggc tctgcaggtc ctccgccatc
      841 ccggtgacca gaccaaacag attggacacc gtcagcgacg gtctggcact gctgcccttc
      901 ccgttcatct caaaaccgct gccgtcaatc gggtatacct gatattgccg cccctgccag
      961 gtgaccgcct cccctttttc attcagctca ttacagaaaa aataccgctc accgccctgc
     1021 accgtcaggt caatttccct gagcaccacc cgcggtgact gctctgattt aaccgactcg
     1081 ttcagacttt cttcgtgaat atcctgcatc agttcaccac ctgcttaaac tcggcactga
     1141 attcaacccg cagcatgcga acccgtgatg accaggcggc acaggtcacc tttatctgcc
     1201 gccaggcata aggcggtgtc cacagaaacg ccttccagcc
//
LOCUS       DAZKIF010000361         1199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZKIF010000361 DAZKIF010000000
VERSION     DAZKIF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.365"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(125..196)
                     /locus_tag="ACHOTW_005112"
                     /pseudo
     CDS             complement(125..196)
                     /locus_tag="ACHOTW_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            190..1184
                     /locus_tag="ACHOTW_005113"
                     /pseudo
     CDS             190..1184
                     /locus_tag="ACHOTW_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          367 a          283 c          284 g          265 t
ORIGIN      
        1 agaagagggc gataacatac atttcaacct tcaaaataaa cctatctaat tattcctaac
       61 agacatcccc catacatgac aacaaaaacc ggagccggac tccggttttt gtgaagctat
      121 cgggttactt catttcgcca atattttccc acttcccgtc agcacgcagg atttgcagcg
      181 gtcttactac acactgaccc gaggacgaaa aaagagttcg ggctgggaag agacagaaga
      241 gtagcaattt cagaagcatt cacaccaaca ttgagctttt cagcgaaagt gggcacgaat
      301 cattgctgga cagggttaaa ggcactgact caatcactct tcatacgtgg cttgatcgat
      361 atgaaacaat cctcagctag agggggagca agccgaaaac attactcgac tacgccagca
      421 aaatcagggc aattcgaaga aaattgccgg acaaaccgct cactgacata tcaacgaaag
      481 aggtggcagc aatgctaaac acctacgtcg cagaaggtaa agcggtttcc gcaagagtaa
      541 tcaggtcaac ccttgttgac gtttttcgag gggcaatagc cgaggggcat gtggcaacga
      601 atccagtaac aacaacccgt gcagcaaagt cagaagtaag gcgctcaagg ctgacagcta
      661 atgagtatgt cgctatttac catgctgctg agcacctccc catctggctg aggctgtcaa
      721 tggatttagc tgtcgttaca gggcagagag tgggcgattt gtgcagaatg aagtggtcag
      781 acataaacga tggtcatctt cacattggac agagtaaaac aggagccaaa attgccattc
      841 cgctggctct aaccattgac gcactcgaca tctcactggt tgatacacta cagaaatgca
      901 gggaggccag cagcagtgaa acaataatcg catcaaccta tcacgaacca ctttctccag
      961 ccacagtatc acggtattta acaaaggcgc gaaatgcatc cgggatctcg tttgatggag
     1021 acccaccgac atttcatgaa ctacgtagtc tgtccgcgag gctatatcgg aaccagattg
     1081 gctacaagtt tgcacaacgt cttcttggac ataaatctga ttcaatggcg gcgcattata
     1141 gggacagtcg cgggcgggag tgggacaaaa ttgaaatcgg ataatgattt tattttgac
//
LOCUS       DAZKIF010000362         1197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZKIF010000362 DAZKIF010000000
VERSION     DAZKIF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.366"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..529)
                     /locus_tag="ACHOTW_005114"
     CDS             complement(<1..529)
                     /locus_tag="ACHOTW_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9847008.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGEGLPHP
                     LTREPITASIIVKHEE"
     gene            complement(642..911)
                     /locus_tag="ACHOTW_005115"
     CDS             complement(642..911)
                     /locus_tag="ACHOTW_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9847009.1"
                     /translation="MNILRKLMERLCGCGKHDDCEHGQSLTVQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCMVRPLQILRADGRWENIGGMK"
     gene            complement(913..>1197)
                     /locus_tag="ACHOTW_005116"
     CDS             complement(913..>1197)
                     /locus_tag="ACHOTW_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9847010.1"
                     /translation="GPVGATGPQGPKGDPGETQIRFRLGPGNIIETNSNGWFPDTDGA
                     LITGLTFLAPKDATRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          314 a          346 c          228 g          309 t
ORIGIN      
        1 attcttcatg ttttacaatt attgatgccg ttattggttc ccgggtcagt gggtggggta
       61 agccttcacc aaccaaacga gaaaatgcag cggcatcaaa taaagtacat acatctgaac
      121 catctgaatt cttcacaaaa ataccttttt caggctgctc cagtgtaatt ggacactgaa
      181 tagcctcaag cgggcactga agtctctctg gacaaacaga aaacttacat tgagatattt
      241 tgttctgaat actctcttgc gattgtgctt cacctgagtc cgaaagcata cttagcattc
      301 tctcaccaag ggctcgagga ccactattaa atcccaaaag caataactca gaaagcaatc
      361 cgccattacc gccactggat gaaaagcgcc cattagtaga atcatacaca atactgacag
      421 tttcaccacc gactgtaata tctccgcgcc cattggctac aattcgggca cgaacaacct
      481 ccaccccaag attaaatgaa tgtgatctaa tatctgaggt taatggcata acaaagctcc
      541 ctattttaat ttgaactcca gacttaaata gctgtaacaa acatccccca tacgtgacaa
      601 caaaaaccag agcaggactc cggtttttgt gaacccgtcg gctatttcat cccgccaata
      661 ttttcccacc tcccgtcagc acgtaggatt tgcagcggtc ttaccatgca ctgtatctgc
      721 tttttatccg catccagtat caccacctgt gtgattaccc tgtcctgctc cggaataatg
      781 ccattctcat ctgactccag gatgtctgcc ggccccagtc gcagttgtac tgtaagcgac
      841 tgcccgtgtt cacagtcatc atgctttccg caaccacaca gacgctccat aagctttctc
      901 aaaatattca tgtcattctc ctgttctgcc tgtatcactg cccacttcat ccagcccctt
      961 aacatcctgc cacggcccgt caccaaacct gacctgcaaa tgctgaaaaa aaccctgaac
     1021 ccgtgtggca tctttggggg caagaaaggt cagtccggtg atgagtgcgc catctgtatc
     1081 cgggaaccag ccattgctgt ttgtctcaat aatgtttccc ggccccagac gaaaacggat
     1141 ttgtgtctcc cccgggtcgc ccttcggtcc ctgaggtccg gttgccccca ccgggcc
//
LOCUS       DAZKIF010000363         1189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZKIF010000363 DAZKIF010000000
VERSION     DAZKIF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.367"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            5..637
                     /locus_tag="ACHOTW_005117"
     CDS             5..637
                     /locus_tag="ACHOTW_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9847011.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGAELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
     gene            complement(656..1159)
                     /locus_tag="ACHOTW_005118"
     CDS             complement(656..1159)
                     /locus_tag="ACHOTW_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFP9847012.1"
                     /translation="MRSPKVKFLTIFTLSILITKMSFASSACFNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKKNNIKSFDYGLMQINQMHIPMLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYKHFSRCGMTWQCLGTYNAGFAMDNQKKRLQYAKKIYIVYTRLNELD
                     NRKALAK"
BASE COUNT          378 a          192 c          227 g          392 t
ORIGIN      
        1 atatatggga aaaattaaaa ttgtagtttc agatcagcag ccgtttatga ttgatgggat
       61 aattggattc ctcggacatt atcccgattt atatgaggtt gttgggggct ataaagatct
      121 gaagaaagct atagcggagt gtaacaaatc tgcagcccaa atatttattt taggagaatt
      181 tgcaggtgga atgatggggg ccgaactcgt aaaatgggtt aaatcgcata aaatagatgc
      241 tcatattatt acatttgtag caaaaatgcc gtatattgat tcaataaaat tgcttgaagc
      301 aggtgcgaaa ggatgcgtat ggaaaaccag tcacccggcg aaacttaatc gcgctattga
      361 ttcgattagt aacggctaca cttattttga tagtgtacat atggattgtg agaaaatatc
      421 ttccagatat tcctctgata atcaacttac aaatcgtgag tctgaaatat tacaactcat
      481 agctgatgga aaaacaaaca aagaaatcgc taatttcttg cagttaagca ggaaaacagt
      541 cgaaactcat agactcaata tcatgaagaa attagatgtt cacagtggta tcgagttgat
      601 caaaacagct ttacgtatgg gtgtgtgcac tatataaaaa aaagaggctt taggctcatt
      661 ttgctaaagc cttacgattg tctagttcat taagcctggt atatacaata tatatttttt
      721 tggcatattg cagtcgcttt ttttggttat ccattgcaaa acccgcatta taagtcccca
      781 ggcattgcca cgtcattcca caacgggaaa aatgcttata taaaatctcg gttcctattt
      841 taatgttcag gcagggatta tctaacaaat ctcgttcatc ttttattatt ccacgttttt
      901 ttaacatggg aatgtgcatc tggtttatct gcattaaacc ataatcaaaa ctctttatat
      961 tgtttttctt atctctgttc ttccctatgc tatccttttt tagattgctt tcaacaagag
     1021 cgatagcttt aatcaaattt ggttcgattc tgaacattgt tcctgcttca ttaaagcaag
     1081 cgctactggc gaaagacatt tttgtaatta atatagaaag cgtgaagatg gttaagaatt
     1141 taacttttgg tgagcgcata tatctttatg agtcaggatt gtataaaag
//
LOCUS       DAZKIF010000364         1183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZKIF010000364 DAZKIF010000000
VERSION     DAZKIF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.368"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..766
                     /gene="sctC"
                     /locus_tag="ACHOTW_005119"
     CDS             <1..766
                     /gene="sctC"
                     /locus_tag="ACHOTW_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFP9847013.1"
                     /translation="IDIDKTDLEQLGADWSGTIKIGSSLSASFNNSGSISTLDGTQFI
                     ATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRTFYTKLVGERTAELDEVTYGTMIS
                     VLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQVGRTLISTIARVPQGKSLLIGGY
                     TRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNIVRVFLIEPREIDERMMNNANEAA
                     VDARAITQQMAKNKEINDELLQKWIKTYLNREVVGG"
     gene            771..>1183
                     /locus_tag="ACHOTW_005120"
     CDS             771..>1183
                     /locus_tag="ACHOTW_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0046903 - secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrpJ domain-containing protein"
                     /protein_id="HFP9847014.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQIS"
BASE COUNT          405 a          205 c          270 g          303 t
ORIGIN      
        1 aatagatatt gataagactg atctagaaca gctaggtgct gactggtcgg gcacgataaa
       61 aattggatcc agcttaagtg catcatttaa taattcaggt tcaattagta cccttgatgg
      121 gacacagttt attgcaacaa tacaggcact tgcacaaaaa agaagagccg ccgttgttgc
      181 tcgtcccgtc gttttaacac aagagaatat tcctgctata tttgataata accgcacttt
      241 ctatactaaa ttagtcggcg agcgtactgc ggaattggat gaagtaacct atggtacgat
      301 gattagcgtg ctaccacgat ttgctgcgcg aaatcaaatt gaattactct tgaatattga
      361 agatggaaat gaaatcaatt ccgacaagac caatgttgat gatctgcctc aggttggtag
      421 aacacttata agtactattg cacgcgtgcc ccaagggaaa agtcttttga ttgggggata
      481 tacacgcgat acgaatacct atgaaagtcg caagatccca atattaggca gcattccatt
      541 tattggtaaa ttatttggtt atgaaggaac aaatgcgaat aacatcgttc gtgtctttct
      601 tattgagcca agagagatcg atgaacgcat gatgaataat gcgaatgagg ctgcggttga
      661 tgccagagcg attacacagc aaatggcaaa aaataaagaa atcaacgatg aattactgca
      721 gaaatggata aaaacttacc tgaatcgtga agtcgtcgga ggatagtgaa atggcgattc
      781 atgtagagca tgtcggtgta ttagaaagag ccagggaagt ttctcgctta gaggacatta
      841 tcacagaaga taatgaagac attgaagctg aaatgcctaa aatgagggat gatcccgcag
      901 gtaaagaagc tcggttctta caagctactg acgagatgtc agcagcgtta acgcagttta
      961 tgaagaaaaa aatttacgaa gaacaactgg ccaattttct ggacggagaa gaatatgtcc
     1021 tggaagacca gccaatagaa aaaaccgaca aagttatgga ggcgcttaag gctgcaacta
     1081 cccatgacta tgaagtgtat agctttgcta aaaaattatt cccagatgaa agcgatctgg
     1141 tagttgtgtt acgtgcaatt ttacgtaaaa aacaaatatc aga
//
LOCUS       DAZKIF010000365         1181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZKIF010000365 DAZKIF010000000
VERSION     DAZKIF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.369"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            20..>1181
                     /locus_tag="ACHOTW_005121"
     CDS             20..>1181
                     /locus_tag="ACHOTW_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9847015.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMK"
BASE COUNT          403 a          174 c          260 g          344 t
ORIGIN      
        1 taagttagca ggtgtcacca tgaaaaaagc catagcatat atgcgatttt catcaccagg
       61 tcagatgtct ggcgactcat taaaccgaca gagaagactt attgctgaat ggttaaaggt
      121 aaatagtgat tattatcttg ataccataac atatgaagat ttaggattaa gtgcattcaa
      181 aggaaagcat gcacaatcag gagctttttc ggaattttta gatgctatag agcatggtta
      241 tatattgcca ggaactacat tgttagttga aagtctggac agactttcaa gagaaaaagt
      301 cggtgaagcg attgaacgtc tgaaattgat tttgaatcac ggtattgatg ttataactct
      361 ttgcgacaat acagtctata atattgactc tttgaatgag ccatattcat taataaaagc
      421 catacttata gcacaaaggg caaatgaaga aagcgagata aagtcaagtc gggttaaatt
      481 atcatggaag aaaaaacggc aggatgcact ggaatcaggt acgattatga cggcgtcttg
      541 tccgagatgg ctctccttag atgacaaaag aacggctttt gttccagacc ccgacagggt
      601 gaaaactatt gagctaattt ttaaactcag gatggaaagg cgctcattga atgcaatagc
      661 caagtattta aatgatcatg ctgtaaagaa tttctcagga aaagaaagtg catgggggcc
      721 ttctgtaatt gaaaaattat tagcgaataa agctctgata ggtatatgcg taccttcata
      781 tcgtgcaaga gggaaaggga taagtgaaat cgctggctat tatcccagag tcatatcaga
      841 tgatttgttt tacgctgtac aggaaattcg gttggcacct tttggtatta gcaatagtag
      901 caagaatcct atgctaataa atctacttcg aacagttatg aagtgtgagg cttgtggtaa
      961 taccatgatt gttcatgcgg tatctggaag tttgcatggc tattatgttt gtccgatgag
     1021 aagattacat cgatgtgaca ggccatcaat aaagagagat ttggttgatt ataatatcat
     1081 taatgaattg ctttttaatt gtagcaaaat tcaaccagtt gaaaacaaga aagatgctaa
     1141 tgaaacttta gagttaaaaa ttattgagct tcagatgaaa a
//
LOCUS       DAZKIF010000366         1180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZKIF010000366 DAZKIF010000000
VERSION     DAZKIF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.370"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..253
                     /locus_tag="ACHOTW_005122"
     CDS             <1..253
                     /locus_tag="ACHOTW_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9847016.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            268..801
                     /locus_tag="ACHOTW_005123"
     CDS             268..801
                     /locus_tag="ACHOTW_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9847017.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            798..>1180
                     /locus_tag="ACHOTW_005124"
     CDS             798..>1180
                     /locus_tag="ACHOTW_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9847018.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          280 a          274 c          359 g          267 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggtgacacgc tgaccattaa cggcgagatg ttctgggtgg accgtgtttc
      181 tccggatgac ggagggagtt gttatctctg gctcaaccgt gggcaaccac cggcagttaa
      241 ccggcgacga taaacgcagg gtgaattatg gcgataaaag ggcttgatca ggcgattgaa
      301 aatctgagcc gggttcgtaa aaacgccatt ccggcggctt cagcaatggc cattaaccgc
      361 gtggccacaa cggcgattaa tcagtcttca tcacaggttg cccgggagac aaaggtacgc
      421 cggaaactgg taaaggaacg gtccagactg aaacgggcca cggtcagaaa tccgaatgcc
      481 agaattatcg ttaaacgcgg tgatctccct gtgattaagc tggggatcag gatgctgggc
      541 cgtcgtccgg acagcatact taaagctggt cagcatcggt atcagcgggc atttatccag
      601 cgattaaaaa atggtcgctg gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc
      661 attgatgtgg tgaaaatccc gatggcggcc ccactgaaac aggcgtttga tgagaatatt
      721 gaccgtatcc ggcgtgaacg tctgcccgga gaactggcat acgcgctgaa acaacaactt
      781 aggattgcga taaaacgatg aaacatactg atatccgtgc ggcagtgctg gatgcactgg
      841 agctgcatga acacggggcg acgctgtttg atggtcgccc cgttgttttt gacgaagagg
      901 attttccggc cgtcgcggtt tatctgacgg atgcagagta taccggtgaa gagctggatg
      961 cagatacctg gcgggccaca ctgcatattg aggtgttttt accggcacag gtaccggatt
     1021 cggagcttga tcagtggatg gaaagccgga tttatccggc ggtgactgcg atcccggcac
     1081 tggcagacct gattacgacg atggttacgc agggctatga gtatcgtcgt gatgacgata
     1141 tggcgttatg gagttctgcg gatctgactt attccattac
//
LOCUS       DAZKIF010000367         1153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZKIF010000367 DAZKIF010000000
VERSION     DAZKIF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.371"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            35..>1153
                     /gene="espG"
                     /locus_tag="ACHOTW_005125"
     CDS             35..>1153
                     /gene="espG"
                     /locus_tag="ACHOTW_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFP9847019.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAA"
BASE COUNT          362 a          245 c          230 g          316 t
ORIGIN      
        1 attgttcatt acaaaccaga taggagaatc tctcatgata aatggactta ataatgactc
       61 cgcatcttta gttttagatg ctgcaatgaa agttaattct gggtttaaaa aaagctggga
      121 tgagatgtca tgcgctgaaa agttatttaa agtacttagt tttggtttat ggaatccaac
      181 gtacagtcgt agtgaaagac aatcatttca agagttgtta accgttttag agcctgtata
      241 tccacttccc aatgaattag gcagagtatc tgctcgtttt tcagatggtt catccttaag
      301 aatttccgtc actaacagcg aacttgttga agccgagatt cgcacagcaa ataatgaaaa
      361 gattactgtg ctcctggagt caaacgaaca aaataggtta ttacaatctt tacccatcga
      421 tcgccacatg ccatacattc aggttcatcg tgccttatct gagatggacc tgactgatac
      481 tacctcaatg cgcaatctac ttggttttac gtcaaaacta tcaacaacct tgattcctca
      541 taatgctcaa acagatccgc tttccgggcc tacaccattc agctctatct ttatggatac
      601 atgtcgagga ctcggcaatg caaagctttc actcaatggt gttgatatac ctgcaaatgc
      661 acaaaaattg cttcgcgatg cactaggact taaagacaca cattcatcac caacccggaa
      721 tgttatagat catggtattt ctcgccatga tgcagagcaa atagcaagag aaagcagcgg
      781 cagtgataaa cagaaagctg aagttgtgga atttttatgc catccagaag cagcaacggc
      841 catatgctcg gctttctatc aatctttcaa tgtgccagcc ttaacgttga cacatgaaag
      901 gatctctaaa gccagtgaat acaatgcgga aagatcatta gatacaccta acgcttgcat
      961 taacatcagt atctctcaat catcagatgg aaacatttat gttaccagcc atactggggt
     1021 tctgataatg gcgccagaag accgccccaa cgagatgggc atgttgacga acaggacttc
     1081 ttatgaagtg ccgcaaggtg tgaaatgtat aatcgatgaa atggtaagtg cgctacaacc
     1141 aaggtatgcc gca
//
LOCUS       DAZKIF010000368         1153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZKIF010000368 DAZKIF010000000
VERSION     DAZKIF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.372"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1153
                     /locus_tag="ACHOTW_005126"
     CDS             <1..>1153
                     /locus_tag="ACHOTW_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9847020.1"
                     /translation="RRTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALI
                     SEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGR
                     YITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTY
                     EDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRK
                     VKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKETIKAL"
BASE COUNT          314 a          243 c          373 g          223 t
ORIGIN      
        1 cgccggacgg ggaaacgggt gtggcgtcgc gggcgtgacc tgacgggatg gatgtcgctg
       61 tcgcggaaac cggaggtgac gtggtacggg tgggacggcg accggctgac gacggtacag
      121 accgacacca cgcgtatcca gacggtatac gagccgggaa gcttcgcgcc gctcatccgc
      181 atcgaaacag acaacggtga gcgggagaaa gcgcagcgcc gcagcctggc ggagaagctg
      241 cagcaggaag ggagcgagga cgggcacggt gtggtatttc cggctgaact ggtgcggctg
      301 ctggacagac tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
      361 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
      421 ccggcgagaa aagttcatct ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
      481 agcgaagacg gcaatacggc gtggagcggg gagtatgatg aatggggcaa ccagctgaat
      541 gaggagaacc cgcatcacct gcaccagccg taccggctgc cggggcagca gtatgataag
      601 gagtcggggc tgtactacaa ccggaaccgg tactacgatc cgttgcaggg gcggtatatc
      661 actcaggacc cgatagggct ggagggggga tggagtctgt atgcgtatcc gctgaatccg
      721 gtgaatggta ttgatccatt agggttaagt cccgcagatg tagcgctaat aagaagaaaa
      781 gatcaactaa accatcaaag agcatgggat atattatctg atacttatga agatatgaag
      841 agattaaatt taggtgggac tgatcaattt ttccattgta tggcattttg tcgagtgtct
      901 aaattaaatg acgctggtgt tagccgatcg gcgaaagggc tgggttatga aaaagagatt
      961 agagattacg ggttaaatct gttcggtatg tacggcagaa aagtaaagct atcccattct
     1021 gaaatgattg aagataataa aaaagacttg gctgtaaatg accatgggtt gacatgtcca
     1081 tcaacaacag attgctcaga tagatgtagt gattatatta atccagagca taaagaaacg
     1141 ataaaggctt tac
//
LOCUS       DAZKIF010000369         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZKIF010000369 DAZKIF010000000
VERSION     DAZKIF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.373"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..441
                     /locus_tag="ACHOTW_005127"
     CDS             <1..441
                     /locus_tag="ACHOTW_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9847021.1"
                     /translation="DEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCD
                     QVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRR
                     GACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            597..779
                     /locus_tag="ACHOTW_005128"
     CDS             597..779
                     /locus_tag="ACHOTW_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847022.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            792..923
                     /locus_tag="ACHOTW_005129"
     CDS             792..923
                     /locus_tag="ACHOTW_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847023.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            931..>1148
                     /locus_tag="ACHOTW_005130"
     CDS             931..>1148
                     /locus_tag="ACHOTW_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFP9847024.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     KLANVTITDMQVRQRDVAALDARYSRELA"
BASE COUNT          288 a          237 c          341 g          282 t
ORIGIN      
        1 gacgaaaaag aaggtaacca caccacggca taccgtgatg gtgcgggtat ctggaccatc
       61 tgccgcggtg ccatcctggt ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag
      121 gaaaaatgcg accaggttaa cgccattgaa cgtgataagg cgctggagtg ggtggagcgc
      181 aatattaaag taccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac
      241 aacattggtc ccggtaagtg tttcccgtcg acgttttaca gacgaattaa tgcaggtgat
      301 cgcaggggag cgtgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt
      361 attcgctcaa acaactgcta cggtcaggtt tcacggcgtg accaggagag cgcgctggcg
      421 tgctggggaa ttgacagata agcagaatat tttgctgaaa aatgcggttt gctcacactg
      481 gcggataaca cgaaatcctg cgaactggca aaaactaagt gaataaaagt aaaaccccgt
      541 ttgttggccg caaacggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
      601 gaaagtataa acgaattctg ttgagattga ctatgaaaaa cggccttgaa ctgaaagcgc
      661 ctgtaactga tgacgtcagc agagcgctgg cttttgctat taagtgggtg gcggtcggta
      721 ttgctgtgtc tccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
      781 gaggattaag catgtcagac aagctcataa cgctggcgaa gatcctctgt gtaatcgtcg
      841 gcatttcatt ttcactaatg ctggttgcta tttgcttttc cactgcctgg cgcgtcttaa
      901 ctttgtcatg gctggtgggg tgagggggat atgaaccgtg ttctgtgtgt ggtgattatt
      961 gtcctgctgg tagcctgtgg tgtgcttagt ctggggctga atcattaccg cgataacgcc
     1021 attacctaca aagcgcaacg cgataaaaaa gccagtgagc tgaagctggc gaacgtgaca
     1081 attactgata tgcaggtacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg
     1141 gaattagc
//
LOCUS       DAZKIF010000370         1145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZKIF010000370 DAZKIF010000000
VERSION     DAZKIF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.374"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            362..991
                     /locus_tag="ACHOTW_005131"
     CDS             362..991
                     /locus_tag="ACHOTW_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFP9847025.1"
                     /translation="MTSQRIPVFNGTIANETTLLVNARDLHTFLGVGKRFASWITERI
                     EEYGFVENQDYIAISQKREIGYGRGKKDYHLTLDTAKETAMVERNEKGRQIRRYFIEC
                     EKKLRNMQSAQAEPQQQFTDEEIILLCYMQLWMEKAQDLSKHLYPIMKELNSSYTNKL
                     YDIAFETIYMVTKNRDVLLREAARLDQSSFVVQRARPMLKSLRARQFEF"
     gene            1039..>1145
                     /locus_tag="ACHOTW_005132"
     CDS             1039..>1145
                     /locus_tag="ACHOTW_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9847026.1"
                     /translation="MADIIDNAAEIEELQRNLSLQKYKSDSNAPSATHC"
BASE COUNT          353 a          274 c          255 g          263 t
ORIGIN      
        1 ttaaggggat ttaacttgat atttcagata attaacgcaa gtttgcgatg cgaatttgcg
       61 ttaattttct ggatgaggca tttaccgtta cccgttcatc aatattccgc tgattgacaa
      121 atcctccaga gctatcgcat actgaccgca ctgaacaaat aagcggtctc cgcacccgat
      181 agctttgcgg cttttttatg cctgcaattt ggcatagtta catccgtaca aaggtcgggt
      241 ggagaggcgt aatacaacac ccgcaagggg aatatgccca gagcttctta tttggctcag
      301 ttgacacccg accgccagct actaactggc ggttataaac taaacaaata aggaggtcat
      361 catgaccagt caacgcatcc ccgtattcaa cggcactatc gccaacgaaa ccactcttct
      421 cgttaatgcc cgtgatttac atacttttct tggagtaggt aaacgctttg catcgtggat
      481 tacagaacgt attgaagagt acggattcgt tgaaaatcag gactatatag ctatttccca
      541 gaaacgggaa ataggttatg ggcgagggaa aaaagactac caccttaccc tcgatacagc
      601 caaagaaacg gcgatggtag agcgtaacga aaaaggccga cagatacgca gatacttcat
      661 cgagtgcgaa aagaaacttc gcaacatgca gtcggcacaa gcagaaccac agcaacaatt
      721 cacagacgag gaaatcatcc tcctctgcta catgcaactc tggatggaaa aagcccagga
      781 cctcagcaaa cacctgtatc cgatcatgaa agagctgaac tcctcataca cgaacaagct
      841 gtatgacatt gcgtttgaga ccatctacat ggtgacgaag aacagagatg tgctactgag
      901 agaggcggca cggcttgacc agtcgagttt cgtagttcag cgggccagac caatgctgaa
      961 aagcctgcgg gcaaggcaat tcgaattcta aaaccaaagg agcttcggct ccttttttca
     1021 tgcctgaagg aaaggaaaat ggcagatatc atcgacaacg ccgctgaaat cgaagaattg
     1081 cagcgcaatc tctccctgca aaaatacaaa tccgatagta atgccccatc tgctactcat
     1141 tgttg
//
LOCUS       DAZKIF010000371         1133 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZKIF010000371 DAZKIF010000000
VERSION     DAZKIF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.375"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            122..793
                     /gene="queE"
                     /locus_tag="ACHOTW_005133"
     CDS             122..793
                     /gene="queE"
                     /locus_tag="ACHOTW_005133"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP9847027.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          305 a          223 c          269 g          336 t
ORIGIN      
        1 tattctttat gatctaatcc actttttgcc ataaacctgc ctgtaacaga atgttaataa
       61 gacatgattg ttgtggcagg atctgcaata attaacaatt atccacttca cagagaatgc
      121 tatgcagtac ccgattaacg agatgttcca gaccctgcaa ggtgagggtt actttaccgg
      181 cgtccccgcc atttttattc gtttacaggg atgcccggtt ggctgtgcct ggtgcgacac
      241 caaacacacc tgggaaaagc ttgaggatcg ggaagtctcc cttttcagca ttctggcgaa
      301 gaccaaagag agtgataagt ggggggcggc gagcagtgaa gacttgctgg ctgtcattgg
      361 tcgccaggga tataccgcgc ggcatgtggt gattacgggt ggtgagcctt gcattcatga
      421 tttgctgcca ctgactgacc tgctcgaaaa gaacggtttt agctgccaga tcgaaaccag
      481 tggtactcat gaagtacgct gtacaccgaa tacctgggtt accgtatcgc caaagctgaa
      541 catgcgcggc ggctatgaag tgttgtcaca ggcactggag cgagccaacg aaatcaagca
      601 tccagtggga cgcgtacgtg atattgaagc actggatgaa cttttggcga cgctgaccga
      661 tgataaaccg cgagtcattg cactgcagcc gattagccaa aaggatgatg ccacacgttt
      721 gtgcattgaa acctgcattg cgcgtaattg gcgtttgtcg atgcaaaccc ataaatattt
      781 aaatattgcc tgattaaaca tttataagcg ttataaatgg gtggaacctg taaagacttc
      841 tactcattta tattctttgt cgtctctgaa aactcatcca tcttgcctat cccggactaa
      901 tcattatcat tctctacaaa ttctgtggcg ttaatttttc gctggagtga aaattattgc
      961 ggtaaagttt ggtagatttt agtttgtata gagttatttt aaatatttac ctttttaatc
     1021 aatgggttaa gtgctcttta acataatgga tgtgttgttt gtgtgatact gtaaagttgg
     1081 tagattgtga ctggcttaaa aaatcattaa ttaataatag gttatgttta gag
//
LOCUS       DAZKIF010000372         1112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZKIF010000372 DAZKIF010000000
VERSION     DAZKIF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.376"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..180)
                     /gene="cesF"
                     /locus_tag="ACHOTW_005134"
     CDS             complement(<1..180)
                     /gene="cesF"
                     /locus_tag="ACHOTW_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesF"
                     /protein_id="HFP9847028.1"
                     /translation="MNEKFRTDLAHTFGIALEEQTDVLSFHDNDGHEWILECASQSEI
                     LFFYCYLLNSESIQIN"
     gene            446..1063
                     /gene="map"
                     /locus_tag="ACHOTW_005135"
     CDS             446..1063
                     /gene="map"
                     /locus_tag="ACHOTW_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HFP9847029.1"
                     /translation="MFSPMTMTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMP
                     VQSNFVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNS
                     VISKETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITR
                     FNTQTKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
BASE COUNT          370 a          200 c          233 g          309 t
ORIGIN      
        1 attaatctgt attgattcgc tattcaataa ataacaataa aaaaacagaa tttcagattg
       61 agatgcacac tccagtatcc attcatggcc atcgttatcg tggaaagaga gcacgtcagt
      121 ttgctcttca agagctatcc caaacgtatg tgcaaggtct gtgcgaaatt tctcattcat
      181 attgtagtcc tgccattctt ttcatgttga tatactcaaa cgtaattaat ccgagagttc
      241 tgatacaaat aataaggcgt agatataaga ttacgggaat agtgttgcat attcatgata
      301 gcatatgata aatgtagtgc aactacctgt agataaggcc ggaggataat tcaaatataa
      361 atacaagata attattcttt aaagttattt tttaatggga agcaattttt ttactgtcga
      421 aatataattt ataaaggttt atggtatgtt tagtccaatg acaatgacaa tggcaggcag
      481 atcgttagtt caggcgactg cacagacgct taaacctgcg gttaccagag ctgcaatgca
      541 agcgggtacg ggagccacgg gtatgaggtt catgcccgtg cagtcgaact ttgtgattaa
      601 tcatggcaaa ctgactaacc aactcctgca ggctgtagcc aaacaaactc gtaatggcga
      661 tacccaacaa tggttccagc aggagcagac gacttatata tccagaacag taaatagaac
      721 tctggatgac tattgcagga gtaataattc ggtcattagt aaggagacta aaggtcacat
      781 ttttagagct gttgaaaatg cgttacagca acctcttgat atgaacgggg cacaatcatc
      841 aattggtcac ttcttgcagt ccaataaata ctttaatcag aaagttgatg agcagtgcgg
      901 caaaagagtg gatccaataa cacgttttaa cacccagacg aagatgatag aacaagtctc
      961 acaagaaata tttgaacgca actttagcgg cttcaaagtc agtgagataa aagcgattac
     1021 tcagaatgcg attttagagc atgtacagga tacccgattg tagggataac cttgtcaggt
     1081 ataaattaag cttcctggtg tatagcatgg cc
//
LOCUS       DAZKIF010000373         1096 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZKIF010000373 DAZKIF010000000
VERSION     DAZKIF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.377"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..703)
                     /locus_tag="ACHOTW_005136"
     CDS             complement(<1..703)
                     /locus_tag="ACHOTW_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9847030.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFT"
     gene            complement(709..>1096)
                     /locus_tag="ACHOTW_005137"
     CDS             complement(709..>1096)
                     /locus_tag="ACHOTW_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9847031.1"
                     /translation="RRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSA
                     SFVLSTPTETDGAVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCS
                     KCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT          239 a          349 c          290 g          218 t
ORIGIN      
        1 ccgtaaaggc agatgcgcgc catgcccggt gacgccagag ggagtgtgtg cgtcgctgcc
       61 atttgtcggt atacctctct cgtttgctca gttgttcagg aatatggtgc agcagctcgc
      121 catcaccaca gtaaatggcg gcatgattcg gcaccgatga accaaaacag cacagcagca
      181 catcgcccgg ttgtgctgat gacaacggca cctgatacag ccctgtggcc tccagattat
      241 ccagatagag attctgaccg tgacgccacc agtcatcctc gcgatgaaaa tccggcatct
      301 caatccccgc cagatgataa gcatcccgga acagcgtgta acagtccgtc accccgtgct
      361 caaagcgccg cccggtgaga tgcggcacac agcggaactt atgaatcgtc ccccggcaga
      421 ccagccacca cggcaaatca ctctgcacct gcagccgccg gtcggcctca ctcagccagg
      481 gcagaccacc ggggtggctg tggaccagcg ccacaatctc accctgcatt tctgcctgca
      541 gccagtcttc cggcgacata cggaaatacg cctccggctc accggagata ttcacgcagg
      601 ggaaatatct ttccccctcc ggcgtgctta ccacgaagcc gcacgactcc gctggcgcac
      661 atcgccgggc gtgcgccaga atcgctgatt ctgtctgtgt catgggattt actgcgaaag
      721 tttgttaatg gaaaggaagc cgccaaagtt gccgacgtta ttgcggaact tacaaccgct
      781 caggcatttg ctgcatttat ccttcgtgat atcggacgtt ggctggtcat attcatccgc
      841 gaccgccgga ccgtgataac cgcactcatc gccgcgatag gtccaggtgc aggtgttggc
      901 cagcatgata cgtcccggaa aaacagcgcc gtccgtttcc gtcggcgtgg acagtacaaa
      961 agaggcactc accgcgctca gttcgctgca ctgctcaatg cgccagcggc tgatcacctc
     1021 ctgctccgga tcggcgtcgc tgtttccgtt gacgaagttc accgcatcca gaaaacgggc
     1081 gtaaacctta cgccgg
//
LOCUS       DAZKIF010000374         1083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZKIF010000374 DAZKIF010000000
VERSION     DAZKIF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.378"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(40..195)
                     /locus_tag="ACHOTW_005138"
                     /pseudo
     CDS             complement(40..195)
                     /locus_tag="ACHOTW_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            345..1013
                     /locus_tag="ACHOTW_005139"
     CDS             345..1013
                     /locus_tag="ACHOTW_005139"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9847032.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          312 a          244 c          211 g          316 t
ORIGIN      
        1 caccactttg tctaattcgt cagattctga ccagacggtt caggctgtac gcttacgagc
       61 tactgatgta tcaacacgat tcaacaatac ccgatattta ttccatgcct ccagcaacga
      121 tctttcttcc tccgttgcga tttccagatc tacagcatcc tgcagtggcg caatatactc
      181 actgaattcc tggatgtaga actgtgtggt gacggtcttc cagccattcg gctcctgctg
      241 tatcgcagca taccaggcta tttcaatatc gctatgctgc ggcagcattt aaccccttgt
      301 aattcatcgc cataattgat ttaattcaca aataaaacta taacatggtg aaattaatga
      361 aaaaaaacac agatgatggg gctaaaattt acacaccact taccctaaag ctttatgact
      421 ggtgggtttt gggagtatca aatcgtcttg catggggatg tcctacaaag gaacaccttc
      481 ttccacactt tctggaacat ttaggtaaca accatctgga tattggtgtt ggaactgggt
      541 tttaccttac tcacgtacct gagagtagtc tgatatcttt aatggatttg aacgaagcta
      601 gcctgaacgc ggcatctaca agggctgggg aatcaaaaat taaacataaa attagccatg
      661 atgtttttga accttatccc gcggcgttac atggtcaatt tgattccatt tccatgtttt
      721 accttcttca ctgcctgcct ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg
      781 cgcaggcctt aactgacgat ggaactctat acggagccac aattcttggc gatggagttg
      841 tgcacaatag cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc atcttttcaa
      901 acacaaaaga ttccgaagaa ggcttaacac atatactctc agagcatttc gagaatgtta
      961 aaaccaaggt tcaaggtact gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa
     1021 ccgcagcacg ttcttgcata cgccgtgtct gcggtttaat ttttactctc aaatattgta
     1081 gtt
//
LOCUS       DAZKIF010000375         1041 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZKIF010000375 DAZKIF010000000
VERSION     DAZKIF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.379"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1041)
                     /locus_tag="ACHOTW_005140"
     CDS             complement(<1..>1041)
                     /locus_tag="ACHOTW_005140"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9847033.1"
                     /translation="IKKISINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKLG
                     FIISLPVFLISALNFVFTPFSFRYIFKPFFCILFICSSIVTYATMKYGVQFDKTMMQN
                     IFETNAGEMTSYFNMSVVLWFLFTGILPCGLLLLVNIRYPETWIKGITYRLISMFASL
                     LIIFAIAFFFYKDYASVGRNNSSLNKEIIPTNYIYSGFKYVRDFFVSPGEFRQTGTDA
                     SRTINEKQKPVIMFLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTST
                     AISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLLWIENDGGCKGVCKRIPTIN
                     IEPSNSDNTLCKK"
BASE COUNT          377 a          189 c          156 g          319 t
ORIGIN      
        1 tttcttgcat aatgtattat ctgaatttga tggctcaata ttaattgtag ggattcgctt
       61 acatacacct ttacatcccc cgtcattttc aatccagagt aaatttaccc ccgttctgta
      121 cagaatatcc agtacatttt ccgaatttac cgctttgcgg gaattgaact cttttcgctt
      181 catatctgaa aacatgcatg gaacagatat ggctgtggat gtgccacacg actgcacatt
      241 atgaaaggat ataagttcat tatatttctt tgtaaaatca ttggtaccac gagaatatcc
      301 attcagagca tagttttgac ttcttgctgt ctccccgaca actaaaaaca taattaccgg
      361 cttttgtttt tcatttattg tgcgggatgc atccgttcca gtctgacgaa actcgccagg
      421 agaaacaaaa aaatccctta catatttaaa cccactgtag atataattag taggtattat
      481 ttctttattt aatgaagagt tgtttctgcc aactgatgca taatccttat aaaagaaaaa
      541 cgcaatcgca aatataatca agagagatgc aaacattgat attaaacgat atgtaatacc
      601 ctttatccat gtctcaggat aacggatatt tactaacaac aatagtccac atggaagtat
      661 acccgtaaat aaaaaccaca gtacaacaga catattgaaa tatgatgtca tttcacctgc
      721 atttgtttca aaaatattct gcatcattgt tttatcaaac tgcacaccat atttcatggt
      781 ggcatatgta acaatggaac tacagataaa cagaatacaa aaaaaaggtt taaatatata
      841 cctgaatgag aacggtgtaa aaacaaaatt taatgcagat atcagaaaga ctggaagaga
      901 tataataaat ccaagtttca caccatcaag gttattaata atattaaaca gatgcatcca
      961 gacagggata ttgaacaatg tggcaaaaac aacatctatc attaacatca agaaaagaaa
     1021 attaatactg atttttttaa t
//
LOCUS       DAZKIF010000376         1035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZKIF010000376 DAZKIF010000000
VERSION     DAZKIF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.380"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..928
                     /locus_tag="ACHOTW_005141"
     CDS             <1..928
                     /locus_tag="ACHOTW_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9847034.1"
                     /translation="QRFDRQKIINIKSTNYRFIAKRTFSYRIHVFSRYLKWLCGLVHS
                     SKGIHAKYEVDVFIESIRAHIPRNSSLNMNEISEKSLNEEEIKVLFRLLEIGGIENPF
                     HKEVQVRNRLIFTLLLNLGLRAGELLNLKIDDFDLRDNTLSVVRRHDSKEDGRSYQPL
                     VKTGERVIPLSDELAREIFDYISNSREKMTKRKKHNFLFVAHCTGKNAGEPLSISAYE
                     KVISTLKRASPELYNLSGHRLRHSWNYMYSKGIEGAELEYNRRKDLRNYLMGWSKESI
                     MCDRYNYKYISQQEKDVVLKVYKSVSKIISGV"
     gene            930..>1035
                     /locus_tag="ACHOTW_005142"
     CDS             930..>1035
                     /locus_tag="ACHOTW_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847035.1"
                     /translation="MENKCIESEQIFFAKMNRYSFKLSDKKWQLDKENC"
BASE COUNT          396 a          134 c          215 g          290 t
ORIGIN      
        1 acaaagattc gacagacaaa aaatcataaa tataaaatcg acaaattata ggtttattgc
       61 gaagagaacc tttagttata gaatacacgt tttttcacgc taccttaagt ggttatgtgg
      121 attagttcat tcgtcaaagg ggattcatgc aaagtatgaa gtagacgtat ttattgaaag
      181 tatcagagca catattccaa gaaacagttc attgaatatg aatgaaatat ctgaaaaatc
      241 gttgaatgaa gaagagatca aagtgctttt ccgtctattg gaaattggcg ggattgaaaa
      301 ccctttccat aaggaagtac aggtcaggaa cagactaata tttaccttgc tcttaaattt
      361 aggtttaagg gctggagagt tgctcaatct taaaatagat gattttgatc tcagagacaa
      421 cactttaagt gtagtcagaa gacatgattc aaaagaagat ggaagatctt atcaaccatt
      481 agtaaagact ggagaaaggg ttattccgtt atctgatgag ttagcccgtg aaatttttga
      541 ttacataagc aatagcagag aaaaaatgac aaaaaggaaa aagcataatt tcttatttgt
      601 ggctcattgc accggaaaaa acgcaggaga acccttaagc atttcagctt atgagaaggt
      661 gatatcaaca ctgaaacgag caagtccaga gctatataat ctttcaggcc ataggttaag
      721 gcactcttgg aattatatgt attctaaagg aatagaaggt gctgaattag aatataatag
      781 aaggaaagat ttaaggaatt atttaatggg ttggtcaaag gagtcaataa tgtgtgatag
      841 atacaactat aaatatatat cgcaacaaga gaaggatgtc gtcctaaagg tctataaatc
      901 agtaagtaaa ataattagtg gagtttagta tggaaaacaa atgtattgaa agtgagcaaa
      961 tcttttttgc taagatgaac aggtatagtt tcaaactgtc agataagaaa tggcaactgg
     1021 ataaagaaaa ctgcg
//
LOCUS       DAZKIF010000377         1033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZKIF010000377 DAZKIF010000000
VERSION     DAZKIF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.381"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(432..650)
                     /gene="ydfC"
                     /locus_tag="ACHOTW_005143"
     CDS             complement(432..650)
                     /gene="ydfC"
                     /locus_tag="ACHOTW_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP9847036.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(722..1021)
                     /locus_tag="ACHOTW_005144"
     CDS             complement(722..1021)
                     /locus_tag="ACHOTW_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847037.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDFATTFWARKLDCIHNQFPLE"
BASE COUNT          242 a          268 c          244 g          279 t
ORIGIN      
        1 agccgcattc accgcatcac aaaattcact ttaaaaaggg cggcagagca gtcacggagt
       61 aaaactgata ccgccaaacg tcaccagaaa attgataaca gagggcgttg cagcggggtt
      121 gtcacttaag cgtatggtca acctgacaac ccggtgtcct caacggggga aggaataacc
      181 cctccatact taccgccgcg ccatttcgcg gattgccaca accggaagcg cacggtcgac
      241 gaaaatttaa cgacaggcta tctatgaacc agctacctcg ccgtgcgctt tcgcgttatg
      301 gtctgacttt tcagggaaat atcctttcag taaactgtca gtgccgtatg ctcacccgtg
      361 tccggcgcac gcactccacc tcacccgtgg agaactcctt aattaccaac cttagcttcg
      421 ttggttagct attaacgcgg gtatgtaatc attctggcaa tgcttaatgc cgctgctttt
      481 tccagattgg tgatatcctg ctccagagcg gacagatttt cagcctgctt agccctggct
      541 tcattggccc atttcagatc ctgcgctgca ttaattttct ggtgcatcca ctcataaagt
      601 tcatcatcgg tatagtctgg cgcgatgatg acgggttctc gtttctgcat gtcggctcct
      661 tgtggttagc gttgcctgct tttaaccacg tcaggcgagg tggtatcctc tgaggggtct
      721 gttactcgag aggaaattgg ttatgaatac aatcaagttt tcttgcccag aatgtggtgg
      781 cgaagtcttt gacacatcct ttaagccgca gggctctgac agtttcgcgg gagccatctg
      841 caaaaattgt ggtcaccttg taactgaaga tgagtcctcg cagttcgatg acgaaatcgt
      901 tgacaatatc ttcggtgcac tcaccagaga ctttctgaag taaaggcgca taccgcttag
      961 ttaccgctct gataactctt acctgtcctg caatggcgct gatgtcaata taaagcgcca
     1021 ttgctgcttc ttt
//
LOCUS       DAZKIF010000378         1009 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZKIF010000378 DAZKIF010000000
VERSION     DAZKIF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.382"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1009
                     /locus_tag="ACHOTW_005145"
     CDS             <1..>1009
                     /locus_tag="ACHOTW_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9847038.1"
                     /translation="AGYQYVLANPGWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQ
                     SRLAQMQEKAQSIQDVLAGLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGL
                     GNELLQQRQGLVNVPLRLPQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAAD
                     DLGFVGDPYQEARQRYISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQ
                     LALVGQNTELAKLKYQTALGELKTLTEMQKQELLRNATLIDQQKIREQLRSREETLKN
                     ENAAARASNDAELLGYGQGERARERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYR
                     QA"
BASE COUNT          274 a          231 c          328 g          176 t
ORIGIN      
        1 tcgcaggata tcagtatgtt ctggcgaacc cgggctggac gaccggtgac ggattcatga
       61 taaaccatct gacatcggtg aagaccgtaa cggaagggct tgctcaggca acagagcagc
      121 ttgccgttga gcagtcccgt ctggcacaga tgcaggaaaa agcgcagtcc attcaggatg
      181 tgcttgccgg gctggaagac cgtcgtgtgg cgttaattcg tcagcaggcg gcagagcaga
      241 ataaggtgta ccagtccatg ctggttatga acggtcagca tacggaattc aaccgtctgc
      301 tggggctggg gaatgaactg cttcagcagc gtcagggact ggtgaatgta ccgttacggc
      361 tgccgcaggc caccctggat gataaacagc agagtgccct gacaaaaaca gagcgtgagc
      421 tggccctgtc cagactgaaa ggggaagaaa aagagcgtgt ccgactgggg tatgcggcgg
      481 atgacctcgg ttttgtgggt gatccgtatc aggaggcgag acaacgttat atcagtaatg
      541 ccctggaagc ctggcgcaat aacgaggcga ataaacccaa atcccggggt ggaaaatcag
      601 agacggaaaa agcggaagac agtttttccc ggctgctgaa gcagcagaaa gagcaactgg
      661 cactggtggg gcagaataca gagctggcga agctgaaata ccagacagcg ctgggtgaac
      721 tgaaaaccct gacggagatg cagaagcagg aactgctgcg taacgcgacc ctgattgacc
      781 agcaaaaaat ccgggaacag ttgcgatccc gggaagagac actgaagaat gagaatgcgg
      841 ctgcgcgtgc gtcgaatgat gctgaactgc tggggtacgg gcagggggag cgagccagag
      901 aacgcatgcg ggagttgcag cagatccgcg acagcttccg ccagaaggat gcggaccttc
      961 agtctcagta tcagaccggg gatatcagtg aggattttta cagacaggc
//
LOCUS       DAZKIF010000379         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZKIF010000379 DAZKIF010000000
VERSION     DAZKIF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.383"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            106..>1003
                     /gene="tdcA"
                     /locus_tag="ACHOTW_005146"
     CDS             106..>1003
                     /gene="tdcA"
                     /locus_tag="ACHOTW_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP9847039.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSS"
BASE COUNT          276 a          192 c          228 g          307 t
ORIGIN      
        1 accctggaaa gtattgaaag ttaatttgtg agtggtcgca catatcctgt tcatttcatt
       61 ttgatacact tcatgccgtc aatgaggtaa ttaacgtagg tcgttatgag cactattctt
      121 cttccgaaaa cgcagcacct ggtagtcttt caggaagtca ttagaagtgg ttctatcggc
      181 tcggctgcaa aagaattagg gttaactcaa ccggccgtca gtaaaatcat taacgatatt
      241 gaagattatt ttggtgtgga attagtggtg cggaaaaata ccggagtaac attaacacct
      301 gccggtcaat tgttactctc tcgttccgaa tccattaccc gtgaaatgaa aaatatggtt
      361 aatgagataa gcggtatgtc ttctgaggcg gtggtggaag tctcatttgg ttttccttca
      421 ttgattggtt ttacttttat gtcagggatg atcaacaagt tcaaagaggt gttcccgaaa
      481 gcgcaggttt ctatgtatga agcgcaactg tcttcgttct taccggcaat ccgcgacggt
      541 cgacttgatt ttgcgattgg tacgttaagc gcagaaatga agcttcagga tttacatgtt
      601 gagccgctgt tcgagtccga gtttgtgctg gtagccagta agtcccgaac atgcaccggc
      661 accaccacgc tggagtcgtt gaagaacgaa cagtgggtgt tgccacaaac taatatgggg
      721 tactacagcg aactgcttac tacgttacaa agaaatggca tcagtattga aaacatcgtt
      781 aaaaccgact cagtcgtgac aatttataat cttgttctca atgctgattt cttaactgta
      841 attccttgtg atatgacgtc tccttttggt tctaatcaat ttattactat tccggttgaa
      901 gaaacattac ctgtggcaca atatgccgcg gtatggtcga aaaattatcg tattaaaaaa
      961 gcagcatcgg ttttggtgga attagccaaa gagtattcat ctt
//
LOCUS       DAZKIF010000380          995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZKIF010000380 DAZKIF010000000
VERSION     DAZKIF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.384"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..878
                     /gene="espX1"
                     /locus_tag="ACHOTW_005147"
     CDS             <1..878
                     /gene="espX1"
                     /locus_tag="ACHOTW_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFP9847040.1"
                     /translation="EIRNLIKKNIYPELYNEQCNKIPASSTDDNQKITWQNFKTSNAA
                     YSQLCEKLSLLKSSPSRLIEKSAYCSNENMITDKFDVVFSYCGDNVKEFILLLPYNKS
                     LEMHELNEQNIQYLTALNINIHKLLLSNITIEKSNLSYGYYFGCVLSNISCFESDLSN
                     TIFSNGEINNLFIKKSNIFGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVF
                     KTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLQS
                     INESMFLMKKISHQD"
BASE COUNT          388 a          179 c          148 g          280 t
ORIGIN      
        1 aggaaatcag aaatctaata aaaaagaata tttatcctga gctatacaat gagcaatgca
       61 ataaaatccc cgcctcatcg acagatgata accagaaaat aacatggcag aattttaaaa
      121 ctagcaatgc ggcatattca caactatgtg aaaaattatc tttattaaaa agctcgccca
      181 gtcggttaat agagaaatca gcttactgct caaatgaaaa catgatcacc gataaatttg
      241 atgttgtctt ttcttactgc ggagataatg taaaggaatt tatactttta ttaccctata
      301 ataaatcgct ggagatgcat gaacttaacg agcaaaacat ccagtatttg actgccctta
      361 atattaatat acataaactg ttgctatcaa atataacaat agaaaagagc aacctttcat
      421 atgggtatta ttttggttgt gtattatcca atatatcatg ttttgaaagc gacttatcaa
      481 acaccatctt ttcaaatgga gaaataaaca acctcttcat taaaaaaagc aacatatttg
      541 gaacatcgtt cactaatacc atgataaaaa atttgcgctg tgaagatatt atgcctggcc
      601 gatggaccac tcaacttgtc aataagcact taggttacag atacacagga gtattcaaaa
      661 ccctggcaag tattgatgac aaaccgtccc gtttcgaaat tttgatacca ttggtacaaa
      721 ctttagttcg cgacaatgta aagctaaata atgatgtgta taaagaactg aaaaagttta
      781 tgcacgatta cgacaaaaca tccccagaaa tgagaaaata ccttcagagc atcaacgagt
      841 ctatgttcct tatgaaaaaa atatctcatc aagattaaaa caccccaaaa aagaagtaat
      901 ttcagaatga aattacctgt gataattatt aattaactgc agagaaataa aaaataatct
      961 gaagcatgct ctgaaacatc ctactctgtc actct
//
LOCUS       DAZKIF010000381          990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZKIF010000381 DAZKIF010000000
VERSION     DAZKIF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.385"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            338..744
                     /gene="ipbA"
                     /locus_tag="ACHOTW_005148"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             338..744
                     /gene="ipbA"
                     /locus_tag="ACHOTW_005148"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
BASE COUNT          312 a          202 c          179 g          297 t
ORIGIN      
        1 taaacatcac taaaacatta gacgtaacac acatccccca cgccctgtaa ttcatagctt
       61 ttatatttat tttttcacat caatgtagat aaaaacacgc attaaaaatg gcttgtagaa
      121 aagcagcaca ataaatagtt catatttagt ctattgcgta gaactactat tcaaaaaatc
      181 ttattaatga gataatgaga ttacatcgat aacgttctga ttgcaggcat acttatctgg
      241 gtgtgcctct cttttttaca actgtacacg gtggcgtatg acgagaaaat atctcacaca
      301 ggatgaagtc tacaggctga tggatgcagc ccagagcatg tcctttcctg aaagaaatcg
      361 ctgtctaatt atgatggctt tcattcacgg ttttagggcc agtgaacttc ttgatttacg
      421 tttatccgat attgatgcat caggaaaaca acttaatatt cggcggatta aaaatggttt
      481 ctcgacaaca catcctcttc ttcctgatga atataatttg atcaagttgt ggcttaagca
      541 gaggaagtta attgaaaacg gagttgaagg agactggctt tttctctccc ggaaacgccg
      601 ccctatcagc agacaacatt tttttctatc attcgtgagg ctggaagacg tgcaggatta
      661 gctgtaaaag cacatcctca tatgttacgt catgcctgtg gttttgctct ggctgacaat
      721 ggtgtcgata cccgactact tcaggattat ctgggtcatc gaaatatcca gcatactgtc
      781 agatacacag ccagtaatgc tgctcgattt aaaggggtgt ggaaaaaaaa gcctcgttaa
      841 aacagtacct tctgattcaa aatgtcagat tcctacgttt tcctcaattc ttccataaaa
      901 aaacagccca ttcaattcaa taaaacacat aacatgttga tatttctcgc taaagtaaaa
      961 taaccccatg atttgaaatg attaaccgcc
//
LOCUS       DAZKIF010000382          980 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZKIF010000382 DAZKIF010000000
VERSION     DAZKIF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.386"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>980
                     /locus_tag="ACHOTW_005149"
     CDS             <1..>980
                     /locus_tag="ACHOTW_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFP9847041.1"
                     /translation="SIPLLQTICPSITTIIKKTEYYGHQLTNAMTVASVVKPYDFSNL
                     GAINSIDKSVSDVPALHTIVEQAKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQ
                     SLLFDRDSKGKCLGLSLLYLDTGGYGGGYQKLRHNIDTASTLYQTKYNDNLKLSNRDD
                     FFLRKTQRIITMSNELGNNRLKNAQLEVLELKDPILTEGILYQRRISSLLITTEYHSL
                     ALQQISSFWRVTDPNFGHCDFHSLAQALTFIKNITSNRNFSSLYGSGIVKIYFSESLN
                     NWKYIKLPLVQTGSLLRDIYLTTPEKLSTSGGSLNIMGHLVPVSFIYDIG"
BASE COUNT          338 a          170 c          161 g          311 t
ORIGIN      
        1 ttctatacca ttattgcaga ccatatgtcc cagcataaca acaataataa aaaaaacaga
       61 atattatggg catcagttaa caaatgctat gactgttgct tcagtggtta aaccatatga
      121 ctttagcaat cttggtgcca taaatagtat agataaatcc gtttctgatg tgcctgcatt
      181 gcatacaatt gttgaacaag caaaatataa tttactttca tggaatgatt tttataacac
      241 tcatgcgtca atatgggata caatagcaag acagcataaa agtacaaata tcgaatttca
      301 cccacaaagt cttctttttg atagagattc gaaaggaaag tgccttggtt tatctcttct
      361 gtatcttgat acaggggggt acggaggcgg atatcaaaaa ctgaggcaca atattgacac
      421 tgccagcaca ttatatcaaa caaaatacaa tgacaatcta aaattatcta accgtgatga
      481 tttcttttta agaaaaacac agcgtatcat tacaatgtcc aatgagttag gtaataacag
      541 gttaaaaaat gcacagttag aggttctgga attgaaagac cctatactaa ctgaaggcat
      601 attataccag cgtcgtatat catctctttt aataacaaca gaatatcatt cacttgcatt
      661 acaacaaata tcttcttttt ggagagtaac tgatcctaat tttggtcatt gtgactttca
      721 ttccttagca caggctttaa cgtttataaa aaatataacg agcaatcgta acttttcatc
      781 tttatatgga agtggcattg ttaaaattta cttcagtgaa tctcttaata attggaagta
      841 tattaagctc ccccttgttc agactggttc tttgttaaga gacatatatt taacaacccc
      901 agaaaaatta tccacaagcg ggggttcatt aaatattatg gggcatctcg tgcctgtcag
      961 ttttatttat gatatcggag
//
LOCUS       DAZKIF010000383          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZKIF010000383 DAZKIF010000000
VERSION     DAZKIF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.387"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            56..856
                     /locus_tag="ACHOTW_005150"
     CDS             56..856
                     /locus_tag="ACHOTW_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847042.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          299 a          180 c          211 g          253 t
ORIGIN      
        1 ccgttatcaa tgctaaatat tctaatcatt atgacaggcg agggagtgtc caattatgaa
       61 ttcaaaaaag ctttgttgca tatgtgtgtt attctcgctg cttgcaggat gtgcctctga
      121 atcttctatt gatgaaaaga agaaaaaagc tcaagtcaca caaagtaata ttaataaaaa
      181 cactccccag caactgacag acaaagattt attcggtaat gaaaccactc tggccgtatc
      241 cgaagaggat attcaagcag cgcttgatgg agatgagttc cgcgttcccc ttaattctcc
      301 tgtaatactt gttcaatccg gcaaccgcgc accggaaacc attatgcagg aggagatgcg
      361 aaaatattat actgtttcca cattctctgg tatcccggac aggcaaaagc ctctgacttg
      421 taacaaaaac aaagataaaa atgagaacga ggatgttgcc cgtgctgaga atatgaactg
      481 gatgcaggca ctgcgttttg tggctgcaaa aggacatcag aaagcgatta ttgtttacca
      541 ggatatgttg cagacaggaa aatatgactc tgcgctgaaa tcaacagtat ggtccgacta
      601 taaaaatgaa aaactcactg acgctatctc cctgcgctac ctggtacgtt tcacgctggt
      661 ggatgtggca acaggtgagt gggctacctg gtcgccggtg aattatgaat cgagagtaat
      721 ttttccgccg gctggaataa caaaaacgag caataaagaa ctatcaaatg accatgttac
      781 ggaagcgcaa ctgtttaatt tgaaacaaaa aacatatgta tcgatggtga aagacttggt
      841 gagtcgattc cagtaatcgt tattgttatg ctgccgcctg tagtttaggg tggcagcaaa
      901 caggagttga gtatttctat aatgtaaata tgattgtttt tat
//
LOCUS       DAZKIF010000384          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZKIF010000384 DAZKIF010000000
VERSION     DAZKIF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.388"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..180)
                     /locus_tag="ACHOTW_005151"
     CDS             complement(<1..180)
                     /locus_tag="ACHOTW_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP9847043.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEAS"
     gene            complement(192..>943)
                     /locus_tag="ACHOTW_005152"
     CDS             complement(192..>943)
                     /locus_tag="ACHOTW_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9847044.1"
                     /translation="EGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYY
                     NRHRYYDPLHGRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDLIHSV
                     ADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTG
                     KGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAIT
                     TKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          247 a          221 c          171 g          304 t
ORIGIN      
        1 tgatgcctct gaatcaccat gttgtgattt atacttcagt ttcatcaact tttcttcact
       61 taaatcatag gtcattgctg tagaccccat catactttcg ttttgtttta ttttcacatc
      121 agcaccatgc aattgacaaa taaaagagaa ataaaaaaat agtaacaagc atgctttcat
      181 acatctacaa cctatcttat atcactgggt ttttctggcg taccttgcat attagaaatg
      241 gggtgcccag atggcccata tgttatccaa ttaccaaggt cttttgttgt tatagccatt
      301 agatccttat ttgaattaag gttcgttggt gctgttagca ctgtatccat ttttaactta
      361 ccattagttc ctcgccccca ccacgtccat agcgtgtcag gtcctattac atttgtatgt
      421 aactctttag ctaattggct agcaaatgaa ttttgtcctt tacctgtatt acaagaaaat
      481 aaccaaactg tcatcccatc tttataattc ccatcaaatt taattagata tgctagatct
      541 ttagctgtca tgatactgcg cgttgcggat tcaatagatg tgggggtacc atgccccccg
      601 attgtgaaaa cgcctgggat attaatctca tcagctacag aatggataag atcactttcg
      661 gggtataaat ttatatcaac caaaccttgc ggatctacat ttatgaccgg attcaacgga
      721 tactgataaa aattccatcc ccccttcagt ccaatcggat cctgagtgat atatcgcccg
      781 tgcagcgggt cataatagcg gtggcggttg taatacaggc cggactcctc atcatactgc
      841 tgccccggca ggcggataag ctgctgcagc tgatgcgggt tctcttcatt cagcaggttg
      901 ccccattcat cgtattctgc gcaccattct gttgcccctt ccg
//
LOCUS       DAZKIF010000385          932 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZKIF010000385 DAZKIF010000000
VERSION     DAZKIF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.389"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..525
                     /gene="bet"
                     /locus_tag="ACHOTW_005153"
     CDS             <1..525
                     /gene="bet"
                     /locus_tag="ACHOTW_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9847045.1"
                     /translation="ENQQFDGMDFEQDNESCTCRIYRKDRNHPICVTEWMDECRREPF
                     KTREGREITGPWQSHPKRMLRHKAMIQCARLAFGFAGIYDKDEAERIVENTAYTAERQ
                     PERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQIFRRDIRASSELTQAEAVKAL
                     GFLKQKATEQKVAA"
     gene            522..>932
                     /locus_tag="ACHOTW_005154"
     CDS             522..>932
                     /locus_tag="ACHOTW_005154"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9847046.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSR"
BASE COUNT          254 a          247 c          239 g          192 t
ORIGIN      
        1 gaaaaccagc agtttgatgg catggacttt gagcaggaca atgaatcctg cacatgccgg
       61 atttaccgca aggaccgtaa tcatccgatc tgcgttaccg agtggatgga tgaatgccgc
      121 cgcgaaccat tcaaaacccg cgaaggcaga gaaatcacgg ggccgtggca gtcgcatccc
      181 aaacggatgt tacgtcataa agccatgatt cagtgtgccc gtctcgcctt cggatttgct
      241 ggtatctatg acaaggatga agccgagcgc attgtcgaaa ataccgcata cactgcagaa
      301 cgtcagccgg aacgcgacat cactccggtt aacgatgaaa ccatgcagga aattaacact
      361 ctgctgatcg ccctggataa aacatgggat gacgacttat tgccgctctg ttcccagata
      421 tttcgccgcg acattcgtgc atcgtcagaa ctgacacagg ccgaagcagt aaaagctctt
      481 ggattcctga aacaaaaagc cactgaacag aaggtggcag catgacaccg gacattatcc
      541 tgcagcgtac tgggatcgac gtgagagctg tcgaacaggg agatgatgcg tggcacaaat
      601 tacggctcgg cgtcatcaca gcttcagaaa ttcacaacgt aatagcaaaa ccccgatcag
      661 gaaagaagtg gcctgacatg aaaatgtcct acttccacac cctgctggct gaggtttgca
      721 ccggtgtggc tccggaagtt aacgctaagg ctctggcctg gggaaaacag tacgagaacg
      781 acgccagaac cctctttgag ttcacttccg gcgtgaatgt tactgaatcc ccgatcatct
      841 atcgcgacga aagtatgcgc accgcctgct ctcccgatgg tttatgcagt gacggcaacg
      901 gccttgagct gaaatgcccg tttacctccc gg
//
LOCUS       DAZKIF010000386          929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZKIF010000386 DAZKIF010000000
VERSION     DAZKIF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.390"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..507)
                     /gene="yhaB"
                     /locus_tag="ACHOTW_005155"
     CDS             complement(<1..507)
                     /gene="yhaB"
                     /locus_tag="ACHOTW_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFP9847047.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAYFNKTSIT
                     SDCWQILFF"
     gene            complement(763..>929)
                     /gene="tdcR"
                     /locus_tag="ACHOTW_005156"
     CDS             complement(763..>929)
                     /gene="tdcR"
                     /locus_tag="ACHOTW_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFP9847048.1"
                     /translation="AARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDR
                     TSFCECNRFP"
BASE COUNT          285 a          169 c          176 g          299 t
ORIGIN      
        1 aaaaaagaga atttgccaac aatctgaagt aatactggtt ttattaaagt acgcaggggg
       61 tgttaagatg gcttcttttc gggtgaattt tgcacaatac ttagaaatcg tctccttaat
      121 acattccagc tcttttttat tgacggctga gcaagtacat ttttcgccaa aaggtgcaaa
      181 agtgcctaca tctaatgttt gtatgatata atcgtcatgt tctcctgtag aaatatctat
      241 gatgaatgga ctcatatttt ccggttggat tcggatcatg acagaaggaa catcgccttg
      301 atagaaattc aatgcagtaa ttgcagataa tactctccgc agttcattac ttatactgta
      361 aattttatta acgtcattgg tggagacaat ttctgcaaac tttcttttta ccgtccaata
      421 atcaatattt ctattgtgat tgttaaccac tgcgtgcatt ggtgggattg aagggtgaaa
      481 aatatgtgca attgggaagc ctttcattga tgcctgcctt gtcatcatga caaacaataa
      541 tagatgctta ttttcctcgc agggttcttt cgcactatca aaaaacgctc atttacccgg
      601 cgttacagta aacgaagcaa cattctgtgg cctacgccga tcaaaagctc tgtgaaaatt
      661 tcccttgttg tctttgtctg accttatcgc ggcgatcagc aattattcgt tttgtgatcc
      721 tcaacacaaa atgctattcg tcgcctatga aggaagaaga atttatggaa aacgattgca
      781 ttcacaaaat gacgtcctgt cagaagagaa aaagttgacg ttaatcaatt gttcgagatg
      841 atcaatacct gtattcgaaa ttttaaaata ttgataaaat aaatgtttgt tctccaacaa
      901 atagcgtgaa aaaatgtgac gtgccgcat
//
LOCUS       DAZKIF010000387          917 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZKIF010000387 DAZKIF010000000
VERSION     DAZKIF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.391"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..785
                     /gene="yeeO"
                     /locus_tag="ACHOTW_005157"
                     /pseudo
     CDS             1..785
                     /gene="yeeO"
                     /locus_tag="ACHOTW_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
BASE COUNT          190 a          181 c          264 g          282 t
ORIGIN      
        1 atattggcgc agttgcaatt ttgtgggtgc tggcgattgg ttttaatcct gcgctaagga
       61 tttcgttaaa gagctatttt aaaccgctga attttagcat tatctgggaa gtcatgggga
      121 ttggtattcc agcgagtgtc gaatcagtgt tatttaccag tggtcggtta ttaacccaaa
      181 tgttcgttgc cggaatgggg accagtgtta ttgccggaaa ttttatcgcg ttttcaattg
      241 cggctcttat caacttaccc ggaagtgcgc tcggttctgc ttctacgatc attacaggcc
      301 gaaggttggg ggtagggcag atagcgcaag cagagattca gttgcggcat gtgttttggc
      361 tgaccactct tggattaacg gccatcgcct ggctaacggc tccctttgcc ggggttatgg
      421 catcgtttta cacctaggat ccacaggtta aacatgtcgt tgtgattctg atttggctaa
      481 atgctttatt tatgcctatt tggtccgctt catgggtgct acccgctgga tttaaggggg
      541 ctcgtgacgc ccgttacgct atgtgggttt ctatgttgag catgtggggt tgtcgggttg
      601 tcgtcggtta tgtgctgggc atcatgcttg gctggggtgt ggttggtgtc tggatgggaa
      661 tgtttgccga ctgggctgtg cgggccgtgc tgttttactg gcgaatggtt actggacgtt
      721 ggctgtggaa ataccctcga cccgaaccgc aaaagtgtga aaaaaagcca gctgtgtcgg
      781 aataaacgac aaaatgcaga ttatttcagc aaacgatttc aaatttaaaa aacaggcttt
      841 gacattgtgg gtgggcatcg ctaatattcg cctcgttctc acgattcctc tgtagttcag
      901 tcggtagaac ggcggac
//
LOCUS       DAZKIF010000388          916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZKIF010000388 DAZKIF010000000
VERSION     DAZKIF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.392"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            280..595
                     /locus_tag="ACHOTW_005158"
                     /pseudo
     CDS             280..595
                     /locus_tag="ACHOTW_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(827..>916)
                     /locus_tag="ACHOTW_005159"
     CDS             complement(827..>916)
                     /locus_tag="ACHOTW_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFP9847049.1"
                     /translation="PPPTGPSGLPPLAQALKDHLAAYEQSKKG"
BASE COUNT          284 a          205 c          175 g          252 t
ORIGIN      
        1 aacaaaaccc cttttaatcc cttatatatc aggctttctt tgtgtacagg ggtcaaccga
       61 ctcaacctgt atcaagttgc ttttgaggat actttagggg gtattatttc ctcttgaaat
      121 attagcaccc cataatggga aaaaggtaat acataacaat ggtttttaac agtatctttg
      181 ttatacaagg gggcattgag gatatcagga aaaatctcaa aatcggtacg gacaggaaac
      241 tacgtgggca gcaaaaacgt atggtaacac cgggaaatgc aacgcccacc agtcgggcta
      301 atccaccact ccaatcgtgg ttcgcagtac tgcacacacg attaccgggc tatacaggag
      361 cggtttggtc tgaaaacatc catgccgcgt aaaggtaacc gttacgacaa cacctcaatg
      421 aaaaacttat agtaaacgct gaagaatgaa actatcgttt taataaccgg gatgaagcca
      481 tctaagtaat acggaaatac attgagattt tctacaacca tcagcgccgc cactcccgtc
      541 tggggaatat ctccctggta gccttcaaga aaaatataac cagataactg cgtaaaaaaa
      601 gaacaaatgg tggtgtccgc tattgccagt atacctgagt ttactgcctt tcagattatc
      661 acaatcaata gcgaacagac ttttaagctc tgtgcgcttt taacaaaacg tccctttctc
      721 cactttcacc gtataataat acattattta ttacccctcc aataaaaacc gatattacta
      781 atcacattta ttaccacaca atttgatatc ggtttttatt agttggttac cctttcttcg
      841 attgctcata ggcagctaaa tgatctttta atgcctgtgc aaggggcggt agtccactag
      901 gccctgtcgg tggcgg
//
LOCUS       DAZKIF010000389          916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZKIF010000389 DAZKIF010000000
VERSION     DAZKIF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.393"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            173..736
                     /gene="fimA"
                     /locus_tag="ACHOTW_005160"
     CDS             173..736
                     /gene="fimA"
                     /locus_tag="ACHOTW_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9847050.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          258 a          175 c          205 g          278 t
ORIGIN      
        1 tgtccttgtg atttaaagat attatcgttc ttcgtgaaat gtaataaccc tggaaactat
       61 attctcaggg caacaaccaa tcaagaatga ttcttgtttt ataagcaata acagaatgac
      121 ttaattgtta actctcttta ttcagctact taaatataaa ttttggagaa ttatgaaact
      181 caaacatgtt ggtatgattg tcgtttctgt gttggcgatg tcgtctgctg cggtaagcgc
      241 agccgagggt gatgaatcag taacgaccac tgttaatggc ggtgttattc attttaaagg
      301 tgaagtggta aatgccgctt gtgcgattga ttccgaatca atgaaccaaa cggttgagct
      361 gggtcaggtt cgttcttctc gcctggctaa agcgggtgac ctcagctccg ccgttggctt
      421 caatatcaag ctgaatgatt gtgataccaa tgtttccagt aatgcagctg ttgcattcct
      481 gggtactact gtcaccagta atgacgatac gttagcgctg cagagttcag cggcaggctc
      541 tgcccaaaat gtcggtattc aaattttgga ccgtacgggt gaggtattaa tacttgatgg
      601 ggccactttt agtgctaaaa ccgacttgat tgatggcacg aatatactac cattccaggc
      661 tcgttatatt gctctcgggc agtccgtagc tggtactgca aacgcagatg cgaccttcaa
      721 agttcaatat ctataattca atattaaggt ggctatacgg catctgaaat taaaacaggg
      781 tgtgtgtggc atggctacgc acacccgagc aattcaccaa tgtgaatctc attattaatg
      841 atgagtggtt tgtatttgga gcgctatgaa aaatcacata atatctaaac gttcaataaa
      901 caacagtagt taaact
//
LOCUS       DAZKIF010000390          908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZKIF010000390 DAZKIF010000000
VERSION     DAZKIF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.394"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            237..422
                     /locus_tag="ACHOTW_005161"
     CDS             237..422
                     /locus_tag="ACHOTW_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFP9847051.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRIIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            663..>908
                     /locus_tag="ACHOTW_005162"
     CDS             663..>908
                     /locus_tag="ACHOTW_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFP9847052.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKI"
BASE COUNT          310 a          160 c          160 g          278 t
ORIGIN      
        1 aagcgagttc agcggaaaac atttcctcga tgtatataat tattattcca attgaaaaaa
       61 atataaagta tctatcacgg cactctcagt tgagtatcac aggcacatgt attttagccc
      121 cctgagtcta cagacaacca atattactta aataagtgat aatcttaatg ctgtttttta
      181 gaacagatga ttgatatttt tgggccggag aaacgcagac aggctttgtt gaataaatcg
      241 agcttttgct gagtttaagg atcagatcac tcattttcct gcaaaagctc attgggcgaa
      301 acaatctgac tcagagaacc cggattaaac gtctggcccg cagaatcatc tatttctcac
      361 gcactgtagt acgtaataaa aaagtcatcg gtacttttat caaaaaacat atattctatt
      421 aattattgcc atcacctttt tcttattgac aacattactc tgctgattgt taaaatctaa
      481 cctgtattca gggttacgat tctatacaat agaacaggga ggggaaagga tgtcatcgtt
      541 aatgagactt tttgttaatg tcaacaatgc tatgaattta ttttagaaaa acaattaagc
      601 tgacgtagtg ttccgtctaa atgacggggc gatattaaca tgattagaac aagaggaatt
      661 ttatgttatc ttcattaaat gtccttcaat ccagcttcag aggaaagaca gctttatcaa
      721 atagtacact tctccagaaa gtttcttttg ctggaaaaga atatcctctg gaacctattg
      781 atgaaaaaac ccctattctt tttcagtggt ttgaagcaag gccagagcga tacgaaaaag
      841 gagaagtacc aatattgaat accaaagaac atccgtattt gagcaatatt ataaatgctg
      901 caaaaata
//
LOCUS       DAZKIF010000391          894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZKIF010000391 DAZKIF010000000
VERSION     DAZKIF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.395"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..250)
                     /locus_tag="ACHOTW_005163"
     CDS             complement(<1..250)
                     /locus_tag="ACHOTW_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9847053.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANS"
     gene            complement(338..880)
                     /locus_tag="ACHOTW_005164"
     CDS             complement(338..880)
                     /locus_tag="ACHOTW_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9847054.1"
                     /translation="MKHSIIAVAVLSSVFMSAGIFAAEVDTGTLTIKGNIAESPCTFV
                     AGGDSVSINMPTVPTTVFDGKAKYSTYDGAVGVTSSMLKITCPKTIDGVKLSLITNEK
                     ILGNDKAISSSNDTVGYYLYLGDSSDVLDISAPFNIESYKTADGQYAIPFKAKYLKLT
                     DSGVASGTVNSSLVMRVAQD"
BASE COUNT          274 a          197 c          165 g          258 t
ORIGIN      
        1 cagaattggc cgctactttc actactggtg gcgttaacat gaaaggaacc tgaatctttt
       61 ctggcggcag agacgtgtcg ccatcatcaa tccacgcctg catcagcgaa gagcggtttc
      121 cctgattcat cagttgtacc atgacctctt tctgctctgc cgggtaaatg atacgggtcc
      181 cgaaaataac cattccggca tgcacgggga atatcataga agagatgagt aaagataata
      241 accctttcat cttaatgcct cttatattta tgctggccgc catgaatatg gcggccattt
      301 taacttactg aaaaaacaag atgattattt taaatattta atcctgggca acacgcataa
      361 ccaatgaaga gttaactgta ccagatgcaa caccactgtc agtcaacttc agatattttg
      421 ccttaaacgg tattgcatat tgtccatcag cagttttata actttcaata ttaaatggtg
      481 cggaaatatc cagtacgtca ctgctatctc ccaaatagag atagtatcca actgtgtcat
      541 tactactgga tattgcctta tcattaccaa gaattttctc gttggtgatc aacgaaagtt
      601 ttacgccatc aatagtcttc ggacaagtaa ttttcagcat actgctggtt acaccaaccg
      661 cgccatcata agttgaatat ttcgctttac catcaaatac agtggtgggt acagtcggca
      721 tattaatact gactgaatca ccgccagcga caaatgtaca tggtgattct gcaatattac
      781 ctttaatggt taaagttccg gtatctactt cagcagcgaa aataccagca ctcataaata
      841 cagaagataa gacagcgaca gcaataattg aatgtttcat gatgcattcc ttaa
//
LOCUS       DAZKIF010000392          891 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZKIF010000392 DAZKIF010000000
VERSION     DAZKIF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.396"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>891
                     /locus_tag="ACHOTW_005165"
     CDS             <1..>891
                     /locus_tag="ACHOTW_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9847055.1"
                     /translation="SASFVPVETESDPMNVTFDNLAPEVQNAVMVKFDTCENITVDMV
                     ISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKWPEIQA
                     EMRIWKKRREGERKEAGKYTSVVDLARARANQQHTENSTGKINPVIAAIHREYKQTWK
                     TLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNGREDWKRISASMRKQPDALRYDR
                     QTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLSSPVPETDAV
                     ETAIPDNEKTECKVEVEPSV"
BASE COUNT          271 a          216 c          220 g          184 t
ORIGIN      
        1 gtctgcttcc tttgtcccgg tcgagactga atccgatccg atgaacgtca cttttgacaa
       61 cctggcccct gaagtacaga acgctgtcat ggttaagttc gacacatgtg aaaacatcac
      121 tgttgatatg gtgattagcg cacaggaatt gttgcaggaa gacatggcaa cattcgacgg
      181 ccatatcgtt gaagcgttga tgaaaatgcc agaagttaac gctatgtatc cggagcttaa
      241 gctgcatgcc atcgggtggg ttaagcataa atgtaagcct ggtgccaaat ggcccgaaat
      301 tcaggcagag atgcgcatct ggaaaaaacg tcgcgaaggt gaacgcaagg aagccggaaa
      361 atacacgtct gttgttgatc tcgcccgcgc cagagccaat caacagcaca ctgaaaattc
      421 aacaggaaaa atcaacccgg tcattgctgc cattcatcgc gaatacaagc agacatggaa
      481 aacactggat gacgaactgg cctacgctct ctggcctggt gatgtggatg ccggaaacat
      541 tgacggcagc atccatcgct gggcaaaaaa tgaagttatc gacaacggcc gcgaagactg
      601 gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc cttcgctacg accgccagac
      661 tatttttggc cttgtccgtg aacgtccgat cgacattcac aaagaccctg tggcactgaa
      721 caaatacatt actgaatacc tgactacaaa gggcgtgttt gaagatgaag gaacaaatca
      781 gagcgcaact gatactctct cgtcgccagt accagaaact gatgcagtgg aaacggcaat
      841 tccagacaac gaaaaaaccg aatgcaaagt ggaagtcgaa ccatctgtag a
//
LOCUS       DAZKIF010000393          883 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZKIF010000393 DAZKIF010000000
VERSION     DAZKIF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.397"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            218..293
                     /locus_tag="ACHOTW_005166"
     tRNA            218..293
                     /locus_tag="ACHOTW_005166"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:251..253,aa:Ile,seq:cat)
BASE COUNT          260 a          159 c          195 g          269 t
ORIGIN      
        1 agtatcattg aatcgatgat aactatagat tgtgctaatt attaacattt gcttgttgac
       61 tgcgagtatc gactttgcgg aatggaggtg gtgtcgggtt ttatgtcgca tgtattgacg
      121 acccaggatg gatgagaacg cataaaaatc tgtgatttac atcataagat catcatgttt
      181 attgcgtggg ttataagtat tgttattgca tacttaaggc cctttagctc agtggttaga
      241 gcaggcgact cataatcgtc cggtcgctgg ttcaagtcca gcaagggcca ccaaatcatc
      301 attcatgtat caggaagagt taagggtaca agggttactc gatggcggtc tatatttcac
      361 gattaacgcc atttcaagga agaagtgcgg tgggtggttt cgaatgaggg ctggtgtttt
      421 ggtacgtggc acgccactgc aatgtattta tctatcagta ctcattcaaa agtatttgga
      481 aaaaatgcct gctatgccaa gcgggcaaat tgcttacatg tggaattaag taattaacga
      541 taaagccagc gtattatcat ttattatacc cattcattta atcaggttaa ttactcaaat
      601 cactgaacta ttctctgttc gctcactgat gataagggag taccacaacc aatgtatgta
      661 gaacaatgcc gtagagtaag gatgaaatgt ctctgccacg ccttaaacat ctactactga
      721 ctagaacacc cgccgcaggt agatgagttt tgtgagcgtt acaggagcgt ggctatgctc
      781 attaatcaac gctatttaga tataaatttc taacactcga aaaacgattt tcactaataa
      841 attataacgg gtggttttta tcatacagcg aaataacctt ttg
//
LOCUS       DAZKIF010000394          874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZKIF010000394 DAZKIF010000000
VERSION     DAZKIF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.398"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(18..761)
                     /locus_tag="ACHOTW_005167"
     CDS             complement(18..761)
                     /locus_tag="ACHOTW_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9847056.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFEREDDWWRNGQNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACT"
     gene            complement(772..>874)
                     /locus_tag="ACHOTW_005168"
     CDS             complement(772..>874)
                     /locus_tag="ACHOTW_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9847057.1"
                     /translation="IRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
BASE COUNT          193 a          279 c          233 g          169 t
ORIGIN      
        1 ggcttccgct gccgtgttca cgtacaggct gaggcggcgg ccaaatcgtt gcaaatcccc
       61 gtgaaggcag atgcgtgcca gtggcggtga cgccagacag aatgcgttcg tcgttgccat
      121 ttttcagaat acctctcccg tttactcagt tgttcaggca gatggtgaag cagttcaccg
      181 ttgccgcagt atatggcggc atgattggcc accgatgcgc caaagcagca cagcaggata
      241 tcgcccgcct gtgcagagga caggggcacc cggtaaaaac cagtcgccgc catattgtcc
      301 aggtaaaggt tctgaccgtt gcgccaccag tcatcctcac gctcaaaatc cggcagcgtt
      361 atccccgcca gatggtatgc atcccggaac agggtgtaac agtccgtcac cccgtgctca
      421 aaacgacgcc cggtcaggtg cggcacacag cggaatttat gaatttcccc ccggcagacc
      481 agccaccagg gcagggcact ctttatctgc agccgccggt cggcctcgct cagccagggc
      541 agaccaccag ggtggctgtg aaccagcgcc acaatctccc cctgcatctc tgcctgcagc
      601 cagtcttccg gcgcaatacg aaaatacgcc tccggctctg cggagatatt cacgcagggc
      661 tggtaccgtt cgccctccgg ggtgcctatc acgaagccgc acgactccgc aggcgcacac
      721 cgccgggcat gcaccagaat cgctgattca gtctgtgtca taaaacagga tttactgcga
      781 aagtttattg atggaaagga aaccgccaaa attagccacc atgccgcgca tctcacaccc
      841 gcgcatgcac ttgctgcatc tgtccttacg gata
//
LOCUS       DAZKIF010000395          874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZKIF010000395 DAZKIF010000000
VERSION     DAZKIF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.399"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>874
                     /locus_tag="ACHOTW_005169"
     CDS             <1..>874
                     /locus_tag="ACHOTW_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9847058.1"
                     /translation="ATNFCNDHISQNSFLRRFNPDYFVTGRINQNNAVNTLYIELTDA
                     KNLFLIASNHLPVDELHNTSQFIIENILQTVHNPQRSVRLTKQDQGYKNHYLSDEMLA
                     GKKELYEFTPESIYRAMTIFDGLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELA
                     AQKALELLDYVSDITTVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYY
                     RALVNFHNEKIEEARICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKKNMKLYYKETE
                     SGSHRVIIDNILKLK"
BASE COUNT          329 a          154 c          148 g          243 t
ORIGIN      
        1 tgcgacaaat ttttgtaatg atcacatatc gcaaaattca tttttgagaa gattcaatcc
       61 agattatttc gttacaggaa gaataaacca gaataatgca gtgaacactt tatacattga
      121 gttgaccgac gctaaaaacc ttttcctcat cgccagtaat catctacctg ttgatgaact
      181 acataataca tcacaattta ttatagaaaa tatccttcaa acagtacata atccacaacg
      241 atctgtaaga ttaactaagc aggaccaagg atataagaat cattatttat cagatgaaat
      301 gttagccgga aagaaagaac tttacgagtt cacccctgaa agcatttaca gggccatgac
      361 tatatttgat ggattacaaa ataaaagtga tatacagacg ctaaaaacag aatgttattg
      421 ccttctagcg gaatgccata tgtctttggc acttcatgga aaaagtgaac ttgaacttgc
      481 tgctcaaaaa gcattagagc ttttagatta tgtatcagac ataaccactg tcgatggaaa
      541 aattttagct attatgggac tgataactgg tctgtctgga caagcaaaag tatctcatat
      601 cttatttgaa caggctaaga tacactcaac tgatatagcc tctctctact actatagggc
      661 acttgtcaac tttcataatg aaaaaattga agaggcaagg atttgtatag acaaatcact
      721 acaactcgaa cccagaagac gaaaagcagt tgtgataaaa gaatgtgtag atatgtatgt
      781 gcctaacccg ctcaaaaaaa acatgaaact ctactataaa gaaactgaga gtggaagcca
      841 tcgagttata attgacaaca ttttgaaatt aaag
//
LOCUS       DAZKIF010000396          863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZKIF010000396 DAZKIF010000000
VERSION     DAZKIF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.400"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..453
                     /locus_tag="ACHOTW_005170"
     CDS             <1..453
                     /locus_tag="ACHOTW_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9847059.1"
                     /translation="NLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEAD
                     PEQELRDRWVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMWTYRSDECGY
                     TGGAVADEFDNPTTDIRKDRCSKCMRGCEMRSMVANFGGFLSINKLSQ"
     gene            464..>863
                     /locus_tag="ACHOTW_005171"
     CDS             464..>863
                     /locus_tag="ACHOTW_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9847060.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAG"
BASE COUNT          161 a          210 c          301 g          191 t
ORIGIN      
        1 aatctgttcg gtcttgtcac cgggatggcg gaggatttgc agagcctggt gggtgccacg
       61 gtggtccgcc gccgggtgta tgcccgtttt ctggatgcgg tgaattttgt ggcgggcaat
      121 ccggaagcgg acccggagca ggagctgaga gaccgctggg tggtggagca gatgtcagag
      181 ctgacggcca tgacagcctc gtttgtgctg gcaacaccga ccgagacgga cggagcgctg
      241 tttcccggtc gcattatgct ggcgaatacc tgtatgtgga cctaccgctc tgatgagtgt
      301 ggttacacgg gcggggcagt ggcggatgag ttcgacaacc ccaccacgga tatccgtaag
      361 gacagatgca gcaagtgcat gcgcgggtgt gagatgcgca gcatggtggc taattttggc
      421 ggtttccttt ccattaataa actttcgcag taaatcccgg tttatgacac agactgaatc
      481 agcgattctg gcgcatgccc ggcggtgtgc gcctgcggag tcgtgcggct tcgtgataag
      541 cacgccggag ggggagtggt atatcccttg tgtgaatatt tctgcagagc cggaggcgta
      601 ttttcgtatc gcaccggaag actggctgcg ggcagagatg cagggggaga ttgtggcact
      661 ggtccacagt catcccggtg ggctgccctg gctgagcgag gctgaccggc ggctgcagat
      721 aaaaagcgca ctgccctggt ggctggtctg ccggggtgac attcacaaat tccgctgtgt
      781 gccacatctg acaggacggc gctttgagca cggggtgacg gactgttaca cgctgttccg
      841 ggatgcttat catctggcgg gga
//
LOCUS       DAZKIF010000397          861 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZKIF010000397 DAZKIF010000000
VERSION     DAZKIF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.401"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>861)
                     /locus_tag="ACHOTW_005172"
     CDS             complement(<1..>861)
                     /locus_tag="ACHOTW_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFP9847061.1"
                     /translation="NTWNIRGNNNFMLTHKGSKCIDFILSGQKKQYLELQRIRDNISY
                     NNLFYTTEDLKSLNNVAIGGIPAKKYLEHGLFSEYRQDGTIPYVVSTLNISGPDMIMR
                     QMKKYYKSLGRIGEVHIKDNKLSDVNFLGVYASSNKDNKSFNWLNPVSVGINDITPDD
                     ESSWAVRNNDINKILFEKINCHVPEKLPTSLYYEIDSRSFFQGWDNKSIKHVTEINKD
                     LIKDINLLLTSSNIDVKLLIKLDRELYAISSKIDNPLALRSIRTLQLQLANYVTSNTF
                     EPENTINFIY"
BASE COUNT          267 a          145 c          121 g          328 t
ORIGIN      
        1 tcataaataa aattaattgt gttttcaggt tcaaatgtat tcgaagttac gtaattagct
       61 aattgtagct gtaaggttct tatagagcgc aaggccaacg gattatctat tttactgcta
      121 atcgcatata attcacgatc cagttttatt aaaagtttaa catcaatatt agacgatgtt
      181 aataataaat taatatcttt gatgaggtct ttattaatct ctgtaacatg ctttatgctt
      241 ttattatccc acccctgaaa aaaagaccgc gaatcgattt catagtatag ggaggtaggt
      301 agtttttcgg gtacatggca gtttattttt tcgaatagaa ttttatttat atcattattt
      361 ctgacagccc atgagctttc atcatctggt gtaatatcat taataccaac actcactgga
      421 tttagccagt taaaactttt attgtcttta tttgaacttg catatacacc caaaaaatta
      481 acatcactca atttattatc tttaatatgc acttcaccta tgcgcccaag agatttatag
      541 tattttttca tttgtcgcat aatcatgtct gggccagata tatttaaagt gctaaccaca
      601 taaggaattg taccgtcctg tctatattca gaaaacaaac cgtgttctaa atatttcttt
      661 gcaggtattc ctcctattgc cacattattt aaagacttta gatcctctgt agtataaaat
      721 agattattat aacttatatt atctctgatg cgttggagtt ctaaatactg ttttttctgt
      781 cctgatagaa taaaatcaat acatttactt cccttgtgag ttaacatgaa attattattc
      841 cctctaatat tccaggtgtt t
//
LOCUS       DAZKIF010000398          858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZKIF010000398 DAZKIF010000000
VERSION     DAZKIF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.402"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..176)
                     /locus_tag="ACHOTW_005173"
     CDS             complement(<1..176)
                     /locus_tag="ACHOTW_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9847062.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHL"
     gene            complement(173..>858)
                     /locus_tag="ACHOTW_005174"
     CDS             complement(173..>858)
                     /locus_tag="ACHOTW_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9847063.1"
                     /translation="GSWMAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQNMAAML
                     TGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTAIQAAA
                     ANFHFAIGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGG
                     AGSPAQMRRAEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDELRLQLRD
                     GGMLSGSGR"
BASE COUNT          178 a          318 c          188 g          174 t
ORIGIN      
        1 tccagatgcc gggcctcctc ccgggtcacg gaaagcgtca cacggtatgt tttcaggtca
       61 gcattcagcc ctgccgccat acgctgtgag tacccgtcac caaaacgcac ttcacgcacc
      121 gatggctgcg agttcacctc catatccggc ttcactttcc agcgaaaggt tttcatcgcc
      181 cgctccccga taacataccg ccatcacgca actgcagccg gagctcatcc tgcgccccct
      241 tgcgggccat gtcatacacc gccttcatca gctgcggccc cgcctgtccg ttggtgccgt
      301 cgttctgaat caccacgtga ttgttctgat taaaattaat accttccgcc cgccgcatct
      361 gcgccggact tccggcacca cccacataac caccttccgc atagccgcgc atcagacggt
      421 aaagattccc cacacctatc cggctggttg cctctttcgt gaaaacaaac tccccgcgat
      481 gaacaattcc cgcaggctca tatttgccgc ccgtccccgt aaatcctccg atcgcgaaat
      541 ggaagttcgc cgccgcagcc tgaatggccg tccccgagga agcagatgca ccaccaccga
      601 aagcaccgcc aatggcgctg ccgatacgcc cgacaatgcc caccatggcc tgtttaagca
      661 ggatttctgt catcatggac agcaccgaac gggtgaatcc ccgccagtct gcctctgcac
      721 cggtcagcat cgccgccata ttctgtgcaa taccgtcaaa ggtctgcgtg gcagcacttt
      781 taacctgcga aaaactgtcc gtcgcacttt ccgcccactc gccccagccg gacttcagcc
      841 cggccatcca gcttccac
//
LOCUS       DAZKIF010000399          855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZKIF010000399 DAZKIF010000000
VERSION     DAZKIF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.403"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>855
                     /gene="espX6"
                     /locus_tag="ACHOTW_005175"
     CDS             <1..>855
                     /gene="espX6"
                     /locus_tag="ACHOTW_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HFP9847064.1"
                     /translation="IECASPEIRSSVLNDLYSIANFLPDNNLTPNESWKRFCETCERF
                     YVAQKSITGDKSERLTRKLSISDAGITMTFKIGDVVINTISTAIPEDATGQRCIEGLN
                     LAEMDLTDIDLSKMALRNVNFNGSILRNAKFSGTICEGVDFTDCDLRNAEFENASLEN
                     NDFRKVRHLTYVNFKNANLRNSNFNGKVLTGVTFTGSDLSNAYLEHIDFTTVILYETS
                     KIPGIPGTPQIPGTPKVILTGAILNYSDLSGKDLSEYNLTGILCMYTNFSNANLTNCK
                     ISNANFSN"
BASE COUNT          278 a          167 c          163 g          247 t
ORIGIN      
        1 ttattgaatg cgcctctcct gaaataagaa gtagcgtcct gaacgatctt tatagcattg
       61 ctaatttcct cccggacaat aatctgaccc caaatgagag ctggaaaaga ttttgtgaga
      121 catgcgagcg cttttacgtt gctcagaaga gtatcactgg agataagagt gaacgtctta
      181 cgcgaaaact ctctatctct gatgcaggaa ttacaatgac cttcaagata ggtgatgttg
      241 tcatcaatac tattagcact gctattcctg aagatgcaac gggtcaacgg tgtatcgaag
      301 ggttgaattt agcagagatg gatttaaccg acatagactt gtcgaaaatg gcgctaagga
      361 atgtcaattt taatggcagc attcttagaa atgccaagtt ctccggtacg atctgtgaag
      421 gcgtggattt taccgattgt gatctgcgta atgcagaatt cgaaaatgcc tcattagaaa
      481 ataatgattt tcgtaaagtt cgccacttga cttatgtaaa tttcaaaaac gcaaatctac
      541 gaaacagtaa cttcaacgga aaagttctca ctggcgtaac ctttactgga agtgacctta
      601 gtaacgcgta tcttgaacac atagatttca caaccgtgat tctatatgaa acatctaaaa
      661 tacctggaat acctggaaca cctcaaatac cgggaacacc taaagtaatt cttactggcg
      721 caatactaaa ttattccgat ctatcgggaa aagatctttc agaatataat cttactggta
      781 ttctctgcat gtataccaac ttttcaaacg ctaatttaac aaattgtaaa atctctaatg
      841 caaacttttc gaatg
//
LOCUS       DAZKIF010000400          847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZKIF010000400 DAZKIF010000000
VERSION     DAZKIF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.404"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>847
                     /locus_tag="ACHOTW_005176"
     CDS             <1..>847
                     /locus_tag="ACHOTW_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9847065.1"
                     /translation="QSIQDVLAGLEDRRVALIRQQAAEQNKVYQSMLVMNGQYTEFNR
                     LLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALTKTERELALSRLKGEEKERVRLG
                     YAADDLGFVGDPYQEARQRYISNALEAWRNNEVNKPKSRGGKSETEKAEDSFSRLLKQ
                     QKAQLALAGQNTELAKLKYQTALGELKTLSEIQKQELLRNAALIDQQKIREQLRYREE
                     TLKNDNVAARASNESELLGYGQGERARERMRELQQIRDSFRQKDADLQSQYQTGDISE
                     DFYRQA"
BASE COUNT          227 a          193 c          279 g          148 t
ORIGIN      
        1 cgcagtccat tcaggatgtg cttgccgggc tggaagaccg tcgtgtggcg ttaattcgtc
       61 agcaggcggc agagcagaat aaggtgtacc agtccatgct ggttatgaac ggtcagtata
      121 cggaattcaa ccgtctgctg gggctgggga atgaactgct tcagcagcgg cagggactgg
      181 tgaatgtgcc gttacggctg ccacaggcca ctctggatga taaacagcag agtgccctga
      241 caaaaacaga gcgtgagctg gccctgtcca gactgaaagg ggaagaaaaa gagcgcgtcc
      301 gactggggta tgcggcggat gacctcggtt ttgtggggga tccgtatcag gaggcgagac
      361 aacgttatat cagtaatgcc ctggaagcct ggcgcaataa cgaggtgaat aaacccaaat
      421 cccggggtgg aaaatcagag acggaaaaag cggaagacag tttttcccgg ctgctgaagc
      481 agcagaaggc gcaactggca ctggccggtc agaacacgga gctggcgaag ctgaagtacc
      541 agacagcgct gggtgaactg aaaaccctgt cggagataca gaagcaggaa ctgctgcgca
      601 atgcggccct gattgaccag caaaaaatcc gggagcagtt gcggtaccgg gaagagaccc
      661 tgaagaatga taatgtggct gcgcgtgcat caaatgaatc tgaactgctg gggtacgggc
      721 agggggaacg agccagggaa cgcatgcggg agttgcagca gatccgcgac agcttccgcc
      781 agaaggatgc ggaccttcag tctcagtatc agaccgggga tatcagtgag gatttttaca
      841 gacaggc
//
LOCUS       DAZKIF010000401          847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZKIF010000401 DAZKIF010000000
VERSION     DAZKIF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.405"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>847)
                     /locus_tag="ACHOTW_005177"
     CDS             complement(<1..>847)
                     /locus_tag="ACHOTW_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847066.1"
                     /translation="RFTKFNSKNNLNSKECLDDSFINFAKAYMLHVHSFNKSKTKHST
                     LSMLKIVEFVLLKINMEANVNYCNNSIYDECIRIASEKYSKAHAFAIGKELEKLSSFL
                     NDNRMTNSFYLFWVNPIRYRITQSWTGYDSSLEGHSRLPDIKSVIAIAEIFSKRDEQL
                     SSRDIFTTSVLALLMCAPSRISEILALPADCEITECDGKGIQRYGLRFFSAKGYEGNI
                     KWIPTLMIPVAKKAISRLKELSSQARLLAAEIQKNYSNSTKGTLKENIPPDLFWYDRE
                     KKIKYS"
BASE COUNT          270 a          158 c          121 g          298 t
ORIGIN      
        1 gaatatttga ttttcttctc tctatcatac caaaagagat caggaggtat attttcttta
       61 agggttccct tcgttgaatt agagtaattc ttttgaattt cagcagccaa taacctcgct
      121 tgacttgata attcttttaa tctgctaata gcctttttag ctacaggtat cattaaagtt
      181 ggaatccatt ttatattgcc ttcataccct tttgctgaaa aaaatcttaa accgtatctt
      241 tgaatgccct ttccatcaca ttctgtgatt tcacaatccg ctggcaaagc taaaatttcc
      301 gatatcctac tcggtgcaca cataagtaaa gcaagcacag atgtagtaaa tatatctctt
      361 gacgataatt gttcatcccg ctttgaaaaa atctcagcaa tcgcaataac tgatttgata
      421 tcaggcaatc tagaatggcc ttccagtgaa gaatcataac cagtccaaga ctgagtaatc
      481 ctatacctaa ttggatttac ccaaaataaa taaaatgagt tagtcatcct attatcattt
      541 aaaaatgaac tcaatttctc aagttctttc ccaatagcaa atgcatgtgc tttagaatac
      601 ttttcagagg ctatccttat gcattcatca tagattgaat tgttgcaata gtttacatta
      661 gcttccatat tgattttaag taaaacgaat tcgacaatct tcaacattga taatgttgag
      721 tgtttcgtct tagatttatt gaatgaatgc acatgcaaca tatatgcttt agcaaaatta
      781 ataaagctat catctaagca ctccttgctg tttaggttgt tttttgagtt aaacttagta
      841 aacctat
//
LOCUS       DAZKIF010000402          842 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZKIF010000402 DAZKIF010000000
VERSION     DAZKIF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.406"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>842)
                     /locus_tag="ACHOTW_005178"
     CDS             complement(<1..>842)
                     /locus_tag="ACHOTW_005178"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP9847067.1"
                     /translation="GPPCQGFSSIGKGNPDDSRNQLYMHFYRLVSELQPLFFLAENVP
                     GIMQEKYSGIRNKAFNLVSGDYDILDPIKVKASDYGAPTIRTRYFFIGVKKSLKLDIS
                     DEVFMPKMIDPVTVKDALYGLPDIIDANWQSDSESWRTIKKDRKGGFYEKLWGQIPRN
                     VGDTESIAKLKNNIISGCTGTLHSKIVQERYASLSFGETDKISRSTRLDPNGFCPTLR
                     AGTARDKGSFQAVRPIHPYHPRVITPREAARLQGFPDWFRFHVTKWHSFRQIGNSVSP
                     IVAE"
BASE COUNT          250 a          178 c          142 g          272 t
ORIGIN      
        1 ttcagcaact attggtgaca cgctatttcc tatttgtctg aaactatgcc atttagttac
       61 atgaaagcga aaccaatcag ggaagccttg taatctagca gcttctcttg gtgtaatcac
      121 tcttggatga taagggtgga tcggcctgac ggcttgaaag cttcctttat ctctagcggt
      181 tcccgccctt aaagtcgggc aaaaaccatt tggatctaat cttgtagatc ttgaaatttt
      241 atcagtttcc ccaaaagata aggaagcata acgctcttgg acgattttgc tatgtaatgt
      301 tccggtacat ccagatatga tattattttt taacttagcg atcgattctg tatcaccaac
      361 attccgaggg atctgccccc ataatttttc ataaaatccc ccttttcgat cttttttaat
      421 tgttcgccaa ctctcagagt ctgattgcca attggcatcg ataatatctg gtaatccata
      481 caaagcatct tttacagtaa ccggatcaat cattttaggc ataaatactt catctgaaat
      541 atcaagcttc aatgattttt ttacaccgat aaaaaaatat ctagttcgaa tagttggagc
      601 accataatca gatgctttta ccttgatggg atcaagaata tcataatcac cgctaaccaa
      661 attaaatgct ttatttctaa tgccagaata tttctcttgc ataatacctg gaacattttc
      721 tgccaaaaag aataatggct gtaattctga tactaaacgg tagaaatgca tgtaaagctg
      781 attcctgcta tcatcaggat tccccttgcc tattgaacta aatccttggc acggaggacc
      841 gc
//
LOCUS       DAZKIF010000403          826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZKIF010000403 DAZKIF010000000
VERSION     DAZKIF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.407"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..143)
                     /locus_tag="ACHOTW_005179"
     CDS             complement(<1..143)
                     /locus_tag="ACHOTW_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9847068.1"
                     /translation="MFPATKGALMRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIAL
                     ERR"
     gene            complement(91..657)
                     /locus_tag="ACHOTW_005180"
     CDS             complement(91..657)
                     /locus_tag="ACHOTW_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9847069.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGAFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          228 a          196 c          133 g          269 t
ORIGIN      
        1 cttcgtcgtt cgagtgctat cttggttcca caaatctcat tacaccaata ttgattttga
       61 tattttggta taaaccattc attgcaacat ttacatttcc ttcgatagat tcgcataagt
      121 gctcctttcg ttgccggaaa aatcaccgta atacttatct cgggcttctt cagcaactag
      181 taccgctaac tccagatcat caaagcatcc gaagtgttta ctcttgccat ggaatcctag
      241 cctaacattc cattttttct gtcgtttgtg ccaagtaacc cctctgcaac ctgatttgct
      301 attctttcgg atccttatat ttcttgaatt ttctattggc aggcattctc ttaaattttc
      361 tggcctattg tcggtcctaa ttccattaac gtggtcaatt tgaccagcag gccaacgatt
      421 atgagttatg taaaaaacta agacgtgagt tttatatcta cgcccatcta tcatgatcat
      481 tgaataaccg ttggaatcaa aagctccagc aacactattt aatgctatcc ttccctgagt
      541 gggaactttc catctaaata ccccggtaga tttatcgaaa cttagtaact caaatatcct
      601 tttaacagtt aaatcttctc ttttacggtt acatcgtctt cgcgctggtt tagccatcgc
      661 cttcttcctc cgtaatggtt ttctgaattt ggccacctga acagagctca ccaaagctat
      721 ggatgtcggt atttccacaa taccaagatg gcgaaaataa ctgcatgata agcctcaggg
      781 aaaagggaag acactacccc cgataattca gaaacaaatc gaaata
//
LOCUS       DAZKIF010000404          825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZKIF010000404 DAZKIF010000000
VERSION     DAZKIF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.408"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(228..802)
                     /locus_tag="ACHOTW_005181"
                     /pseudo
     CDS             complement(228..802)
                     /locus_tag="ACHOTW_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
BASE COUNT          265 a          189 c          127 g          244 t
ORIGIN      
        1 ctaaatacac acacctaatc tcaaaaaaaa caaacttact atttgacttg gattatgttt
       61 ttaatccaat attttccgac aacatctcca cagaaaaatg catttacata aagacgcctt
      121 caagtcatct gaaacctgcc aaccacagac accttcttat attctggatg tctgcggcgt
      181 ttttttacaa attactgaca gccaatactc tctcagagaa aaatatttta actatctttt
      241 ataatgaagt ttcccttgat aggatcataa acacatttat caggacttat aatcattgat
      301 gccgttattg gttctcgggt cagtggatga tatgagcctt cactagctaa acgagaaaat
      361 gcatcaaaat caaataagca gcatactgcc gaactatctg aatttttgac aaacactcct
      421 tcttcgggtc tctctagtgt aattggacac tgaatagact ctggcgggca ctggaaattt
      481 cctgaactaa caggaaactt acattgagat attttgtcct gaatactctc ttgcgattgt
      541 gcttcacctg agtccgaaag catactgagc atcctatcgc gaagagcttc agggccgtta
      601 ttaaacccat aaaggaataa ctcagtaagc aatcccccac tactgccgct ggatagaaaa
      661 cgcccatcgg tttcattaaa tacaatgctg acagtctcat tacctaatgt aaactcccct
      721 cgaccgctag atgctacttg agctcgaagg acattcaatc cattaaaaat gagcttgatc
      781 taatctccga atttaacggc attataaaac tccctatttt aattt
//
LOCUS       DAZKIF010000405          825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZKIF010000405 DAZKIF010000000
VERSION     DAZKIF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.409"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..268
                     /locus_tag="ACHOTW_005182"
     CDS             <1..268
                     /locus_tag="ACHOTW_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847070.1"
                     /translation="TRAYFGELQASIEQDINQPVRQEIDAFFCAFREKVEQLRNTLIQ
                     SSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            265..>825
                     /locus_tag="ACHOTW_005183"
     CDS             265..>825
                     /locus_tag="ACHOTW_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9847071.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGA"
BASE COUNT          203 a          208 c          233 g          181 t
ORIGIN      
        1 gacccgggca tatttcgggg agctgcaggc atccattgag caggatatta accagcccgt
       61 ccgccaggaa atcgatgcct ttttctgcgc attcagggag aaagttgaac aactgcgtaa
      121 cacgctgatt cagagctctg aagatcataa acgcgatcag caggcgcagg aacggcttac
      181 cgggcgactt caggcattaa atgaaagggt tcctgagctc attactgaca gtaaggcgct
      241 gagggaagag ctggagacaa tgctgtgact tcaccattta ttcaacaaat tgctgataac
      301 cgggtatgtc aggtactcac ctgtcttcct gaaaaatttg tggttgattt cgcaaacgga
      361 atcgatgttg cacaggagca tatccgcacg gccggagagc gcacgttctt ccggcgttta
      421 aaagaggggc tgaccggcga aggcgctgca cggcagaatg ccatcaatgc gtcgcttgca
      481 cagggcgttg aggcgtccct gcgctggctg acggagatga caacgtcact tgccaccaca
      541 aactacgcga ttacccgggt aaacgacagg gtcagttcac tggtcagtga tacagccagg
      601 ctggcgcatt attccgcaga tacgcgggag cagttactca ccctggccga tcaggttcac
      661 cataaactga atcatcttga agaaaaactc caccgtgttg accaggttca gcgggcgcag
      721 ttacatcttg agcagatatt ctcatggtgg agcgccgggc gatatgcgtc attttcccct
      781 gccggacgtt gttatgtggc acttgaagag cttcgctggg gcgcg
//
LOCUS       DAZKIF010000406          823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZKIF010000406 DAZKIF010000000
VERSION     DAZKIF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.410"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>823
                     /locus_tag="ACHOTW_005184"
     rRNA            <1..>823
                     /locus_tag="ACHOTW_005184"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          206 a          190 c          268 g          159 t
ORIGIN      
        1 agctaatacc gcataacgtc gcaagaccaa agagggggac cttcgggcct cttgccatcg
       61 gatgtgccca gatgggatta gctagtaggt ggggtaacgg ctcacctagg cgacgatccc
      121 tagctggtct gagaggatga ccagccacac tggaactgag acacggtcca gactcctacg
      181 ggaggcagca gtggggaata ttgcacaatg ggcgcaagcc tgatgcagcc atgccgcgtg
      241 tatgaagaag gccttcgggt tgtaaagtac tttcagcggg gaggaaggga gtaaagttaa
      301 tacctttgct cattgacgtt acccgcagaa gaagcaccgg ctaactccgt gccagcagcc
      361 gcggtaatac ggagggtgca agcgttaatc ggaattactg ggcgtaaagc gcacgcaggc
      421 ggtttgttaa gtcagatgtg aaatccccgg gctcaacctg ggaactgcat ctgatactgg
      481 caagcttgag tctcgtagag gggggtagaa ttccaggtgt agcggtgaaa tgcgtagaga
      541 tctggaggaa taccggtggc gaaggcggcc ccctggacga agactgacgc tcaggtgcga
      601 aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgtcgac
      661 ttggaggttg tgcccttgag gcgtggcttc cggagctaac gcgttaagtc gaccgcctgg
      721 ggagtacggc cgcaaggtta aaactcaaat gaattgacgg gggcccgcac aagcggtgga
      781 gcatgtggtt taattcgatg caacgcgaag aaccttacct ggt
//
LOCUS       DAZKIF010000407          823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZKIF010000407 DAZKIF010000000
VERSION     DAZKIF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.411"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..470
                     /locus_tag="ACHOTW_005185"
     CDS             <1..470
                     /locus_tag="ACHOTW_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9847072.1"
                     /translation="PYLLRTHQLRHLLNTFAQINGMDEFSIARWSGRKLISQNVSYDH
                     RSHLQMSKAIREKKLSVCVNEHRIKDIPVVDLNEFDSLSSGAVLVSKHGYCKHSYAFK
                     PCDNYPIKNSGLDNETISNIHDKILKRTLYDKNDGNINADKWYEFHKKIKKGE"
     gene            471..>823
                     /locus_tag="ACHOTW_005186"
     CDS             471..>823
                     /locus_tag="ACHOTW_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847073.1"
                     /translation="MAKHINGYHEKKIVDVINTWSIDEKLTWNALCDRLVRVIGKRPS
                     RQSLSSHVRIAESFNVKKTTIKSGVIHTVKPANLKVASQRIKRLEAENEALKALNERY
                     LEQFKVWLYNAHLKQ"
BASE COUNT          314 a          123 c          154 g          232 t
ORIGIN      
        1 gcccatattt gctaagaaca catcaactaa ggcatcttct caacacattt gctcaaataa
       61 atggtatgga tgaatttagt attgctcgct ggtctggacg taagcttatt tctcaaaatg
      121 tttcttatga ccacagatcg catcttcaaa tgtctaaagc aataagagaa aaaaagttat
      181 cagtatgtgt taatgagcac agaataaagg atattccagt agtggatctt aatgagtttg
      241 actcacttag tagtggtgca gtacttgtat caaaacatgg ctactgcaag cactcatatg
      301 cgtttaagcc gtgtgataat tatccaatta agaactctgg tttagataac gaaacgattt
      361 caaatatcca cgataaaatt ttaaaaagaa cactgtatga taaaaatgat gggaacataa
      421 atgctgataa atggtatgaa ttccataaaa aaataaaaaa aggagaataa atggctaagc
      481 atataaatgg ttatcatgag aagaagattg tagatgttat taacacatgg tctatagatg
      541 aaaaactaac ctggaatgct ttgtgcgata ggttagtcag ggttattggg aaaagaccat
      601 caagacaatc cttaagtagt catgtaagga ttgcggaaag cttcaatgtg aaaaaaacca
      661 caatcaaatc tggtgtaata cacactgtaa aacctgctaa tttaaaagtg gcttctcaac
      721 gaattaaaag acttgaagca gaaaatgaag ctttgaaggc attaaatgaa cgctatttag
      781 agcagttcaa agtatggctt tataatgctc accttaaaca aat
//
LOCUS       DAZKIF010000408          810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZKIF010000408 DAZKIF010000000
VERSION     DAZKIF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.412"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..94)
                     /locus_tag="ACHOTW_005187"
     CDS             complement(<1..94)
                     /locus_tag="ACHOTW_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847074.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLV"
     gene            175..>810
                     /locus_tag="ACHOTW_005188"
     CDS             175..>810
                     /locus_tag="ACHOTW_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HFP9847075.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITD
                     GEIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLN
                     LLDLKKNLITIDAMGCQKDIASKIKDKKADYLLAVKGNQGKLHHAFEEKFPV"
BASE COUNT          296 a          119 c          188 g          207 t
ORIGIN      
        1 acaccagaaa gagaaaaata taaaatacat acagaaagac atgagagcaa gggacggggc
       61 agggcaagat catgaaaatc gtgatgtaaa tcacgatttt catcctggct ttaacgccta
      121 caggtgatca aaatttaacc tttggaataa ctaaaaagat aaaaaggacg ccaggtgagt
      181 attcaaagtt tgcttgatta tatttcagtg atccctgata tacgacaaca aggaaaggtt
      241 aaacataaat tatctgatat tttgtttctc accgtatgtg cagtaattgc aggtgccgat
      301 gagtggcagg aaattgaaga ttttggacat gaaagacttg aatggctaaa gaaatatggt
      361 gattttgata atggcattcc ggtcgatgac accattgcac gcgttgtgag taacattgac
      421 agtttggcct ttgaaaagat atttattgaa tggatgcagg agtgccatga aatcactgat
      481 ggtgaaatta tagcaataga tggaaagacc ataagaggct cctttgataa gggaaaaaga
      541 aaaggagcaa tccatatggt gagtgcattc tcgaacgaaa atggtgttgt actggggcag
      601 gtaaaaacgg aagccaaaag taatgagatt acagccattc cagagttgct taacctactg
      661 gatttaaaga aaaatttgat aaccattgat gctatgggct gtcagaaaga tatcgcttcg
      721 aagatcaaag ataaaaaagc agattatctt ctggcagtaa aaggcaatca ggggaaatta
      781 catcatgcat tcgaggaaaa atttcctgta
//
LOCUS       DAZKIF010000409          810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZKIF010000409 DAZKIF010000000
VERSION     DAZKIF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.413"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..562
                     /locus_tag="ACHOTW_005189"
     CDS             <1..562
                     /locus_tag="ACHOTW_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847076.1"
                     /translation="FSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSA
                     NQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSN
                     NQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGAN
                     NWTPTTSISDLLVVVMKKSTAGISIS"
     gene            629..>810
                     /locus_tag="ACHOTW_005190"
     CDS             629..>810
                     /locus_tag="ACHOTW_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9847077.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFT"
BASE COUNT          226 a          190 c          202 g          192 t
ORIGIN      
        1 attttccctg acaccggacg gaaagctgac tgctaaaaat gcggatatca gtggcagtgt
       61 gaatgcgaac tccgggacgc tcaacaacgt cacgattaat gagaactgtc agattaaggg
      121 gaaactgtca gccaatcaga ttgaaggcga tattgtcaaa acggtcagca agtctttccc
      181 ccgcacgagc acttatgcca gtggcaccat cacggtaaga atcagtgatg atcagaaatt
      241 tgaccggcag gtcatgatac cgccagtgtt attccgcggt ggtaagcatg agaatttcaa
      301 cagtaataac caacagtcat actggtattc aacctgccgg ttaagagtga cccgcaatgg
      361 ccaggagatt tttaatcagt ccacgacgga tgctcagggc gtattttcct cagttataga
      421 tatgcctgcc ggacagggga cgctgacact gacattcacc gtatcttcat caggagcgaa
      481 taactggaca ccaacaacca gtatcagcga tctgctggtt gtggtgatga aaaaatccac
      541 agcaggtatc agtatcagct gaattttata acccagaacg ggcgtcagaa atgacgcctt
      601 ttttattgca gaaaagcgag aggtaattat gcgtaaactt tatgccgcca ttttgtccgc
      661 agccatttgt ctggccgtat ccggtgcgcc tgcatgggca tctgaacatc agtccacgct
      721 gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc tgaacgggat
      781 taacgtgaaa taccgttatg agtttacgga
//
LOCUS       DAZKIF010000410          784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZKIF010000410 DAZKIF010000000
VERSION     DAZKIF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.414"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(130..>784)
                     /locus_tag="ACHOTW_005191"
     CDS             complement(130..>784)
                     /locus_tag="ACHOTW_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP9847078.1"
                     /translation="NKEDDFLYATQNSPAQLLKITKSGDIMDRAPLPFISDAETIEHI
                     QGNIFAAVDEKTSELFFFTVTKDMHISFRNKIQLEKFNKKNRGFEGLAWKADDRMLFV
                     AKERRPSKIFIYQLSPDLLSVKQATIPEALNDIRVNDISGLAFNNESLMILSDESRKL
                     LKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLPDESIYVASEPDILAKFVPNK"
BASE COUNT          218 a          142 c          153 g          271 t
ORIGIN      
        1 tctggttgat aataactgat atccttttca actattaatg tgtttattta gcgtgaaatt
       61 atactcgtat ttttataact ttatgagatt gccaggtttg gtcgataaat aataaggcca
      121 gtgacagact tacttattag ggacaaattt cgcgaggata tcaggttcac tggcaacata
      181 aatgctttca tcaggcagag ttacaattcc ttcaggttgg ggcaaatcac tggtcagaga
      241 atgatttcct tttgtcaggt caagcatctc aatgaaagac atctctgtca ggttaaactt
      301 gagtaattta cgcgattcat cggacagaat catcagactt tcattattaa aggccaggcc
      361 ggatatatcg ttaacacgta tatcatttag tgcctctgga atagttgctt gctttacact
      421 cagcaggtct ggagaaagtt gatatataaa tattttactt gggcgtcttt cttttgcgac
      481 aaacagcatc cgatcatctg ctttccatgc cagcccttca aaccctctat tctttttatt
      541 gaatttttct aactgaattt tatttcgaaa ggagatatgc atatcttttg taactgtaaa
      601 aaagaatagt tcagaagtct tttcgtctac tgcagcaaaa atgttccctt gtatgtgttc
      661 aattgtttcg gcatcagaga taaaaggcag tggggctctg tccatgatgt cgccactttt
      721 ggttatttta agtagctgtg cagggctatt ctgggtggca tacaggaaat catcctcttt
      781 atta
//
LOCUS       DAZKIF010000411          772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZKIF010000411 DAZKIF010000000
VERSION     DAZKIF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.415"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            149..718
                     /locus_tag="ACHOTW_005192"
     CDS             149..718
                     /locus_tag="ACHOTW_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9847079.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
BASE COUNT          218 a          162 c          200 g          192 t
ORIGIN      
        1 atgcctttgt catttctgcg catctcacgc gcatctcaca acacagaacc tttcaggatg
       61 acccttgagg ataccggttt ggctgtcggt gcctttctgt gggctggatt cctgtgagac
      121 aaggttcatc actaaaagga aataaccgat gaatatgatg gccgtgccgt ttcacggcaa
      181 ctctctttat gtagttaacc ataatggcga accatacgtt cccatgaaac ctgtcgttgc
      241 ggggatgggg ctggcctggc aatcacagtt ggctaagtta agacagcgtt ttgcgtcaac
      301 tataacggaa atcgttatgg ttgctgagga tgggaaacaa cgcaatatgg tgtccatgcc
      361 acttcgaaaa cttgccggct ggctacaaac cattaatccc aacaaagtaa aacccgaaat
      421 ccgcgataag gtcatccggt atcaggaaga gtgcgacgat gttctttacg agtactggac
      481 gaagggtttt gtcgttaatc cccgtaaaat gagcgtgatg gaagaactca accaggcttg
      541 tgctgacatg aaacgggata aaaacattgc cagtgtgttt gctaccgggc tgaatgagtg
      601 gaaacaggtt aaagccgcgc atgtatcaaa aatccgtacg ctggtaaatg aagcgaatat
      661 gctgattgat tttgtcctgg ctgatacagg caaagggaaa ataacaaagg cggattgatg
      721 gggtggctaa tgatatcaga taaactcata acgctggtga agagcctctg tg
//
LOCUS       DAZKIF010000412          771 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZKIF010000412 DAZKIF010000000
VERSION     DAZKIF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.416"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..697
                     /locus_tag="ACHOTW_005193"
     CDS             <1..697
                     /locus_tag="ACHOTW_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9847080.1"
                     /translation="YKAKIDDVDIDLVVGFYKNLEDDNDDVLEKRSSDDAGWTVICND
                     RVVLYCDKTHLTGWGFANVPRFHTQFIAISGVVRFTSKNPEKLPITTTKRGVDLSSTL
                     YNDVRNKMIEGMMHFIRFTNQWKGEHLEEGKKLLKSAQSHEAQSLFEITPQSTPEDKK
                     NNWSNPNRNKNEWRYTPKLPTPVKKSSTVRIIFTREKEDVKILSKYFFGHEDASASDV
                     GMQCFDTVIEEVK"
     gene            697..>771
                     /locus_tag="ACHOTW_005194"
     CDS             697..>771
                     /locus_tag="ACHOTW_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFP9847081.1"
                     /translation="MSGQYIPYHLRHNKSIDREIFLESL"
BASE COUNT          272 a          125 c          152 g          222 t
ORIGIN      
        1 atataaagct aaaatagacg atgttgatat cgatttagtt gttggcttct ataaaaattt
       61 agaagatgac aatgatgatg ttttagaaaa aaggagttct gatgatgctg gatggactgt
      121 catttgcaat gaccgcgttg ttctatattg tgataagaca catctcacag gttggggctt
      181 tgctaatgta ccaaggtttc atacccaatt tatagcaatt tctggggttg ttcgctttac
      241 cagtaaaaac ccagaaaaat taccaataac aacaacgaaa cgaggtgtcg atcttagttc
      301 tacattgtac aatgatgtac gaaataaaat gattgaaggg atgatgcatt ttattcgatt
      361 caccaatcag tggaaaggtg agcatcttga agaagggaaa aaattactta agtcagctca
      421 gtcacatgaa gctcaatctt tatttgaaat taccccacaa tcaactccag aagataaaaa
      481 aaataactgg tccaacccta atagaaacaa aaatgagtgg cgatatacac ctaagctgcc
      541 taccccagta aaaaaaagct ctactgtacg aataatcttc accagagaga aagaagatgt
      601 taaaatttta tctaaatatt tctttggaca tgaagatgct agcgcttctg atgtggggat
      661 gcaatgcttt gatacggtga ttgaagaggt gaagtgatgt ctggacaata cattccctat
      721 cacttaaggc ataataagtc tattgataga gagatttttc tcgaaagcct a
//
LOCUS       DAZKIF010000413          768 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZKIF010000413 DAZKIF010000000
VERSION     DAZKIF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.417"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>768
                     /locus_tag="ACHOTW_005195"
     CDS             <1..>768
                     /locus_tag="ACHOTW_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFP9847082.1"
                     /translation="DKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDD
                     WLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRT
                     TISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRV
                     SSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGD
                     SYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWV"
     misc_feature    2..61
                     /locus_tag="ACHOTW_005195"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          186 a          180 c          239 g          163 t
ORIGIN      
        1 ggataagctg cgtgagcagt acggggtcgg accggtatgc agcgaactgc atattgcccc
       61 gtcaacgtat taccattgtc agcaacagcg acatcatccg gataaacgca gtgcccgtgc
      121 gcagcacgac gactggctga agagagagat acagcgcgta tacgatgaaa atcatcaggt
      181 gtacggtgtg cgtaaagtct ggcgtcagtt gttacgggaa ggaatcaggg tggccagatg
      241 tacagtggca cgtctcatgg cggttatggg acttgccggt gttctccggg gtaaaaaggt
      301 ccgtacgacc atcagccgga aagccgttgc cgcaggcgac cgcgtaaacc gtcagttcgt
      361 ggcagaacga cctgaccagc tgtgggtggc tgattttact tacgtcagca catggcaggg
      421 cttcgtctat gtggcgttta tcattgatgt gtttgccgga tacatcgtgg ggtggcgggt
      481 ctcatcgtct atggaaacga cattcgtgct ggatgcgctg gagcaggcgt tgtgggcccg
      541 tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt cactggccta
      601 tacggagcga ctaaaagaag ccggattact ggcatcaaca gggagtacag gcgactcgta
      661 tgacaacgcg atggctgaga gcatcaatgg tctttacaaa gcggaggtaa tacaccgtaa
      721 gagctggaaa aaccgtgcag aagtggaact ggccacacta acgtgggt
//
LOCUS       DAZKIF010000414          766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZKIF010000414 DAZKIF010000000
VERSION     DAZKIF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.418"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..317
                     /locus_tag="ACHOTW_005196"
     CDS             <1..317
                     /locus_tag="ACHOTW_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP9847083.1"
                     /translation="EGNYIKDNDSMSIRYRSKYYSRDSALIEEETIFSDAELKAILPM
                     RRMYGVSDYKSNSSSLSSHSGLKDPTGTPVCYYIHNESKPSLGYGPTSNNWLSQSFTT
                     EL"
     gene            380..>766
                     /gene="espR1"
                     /locus_tag="ACHOTW_005197"
     CDS             380..>766
                     /gene="espR1"
                     /locus_tag="ACHOTW_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HFP9847084.1"
                     /translation="MKFPSIFNKIKPQSIQQHPEKNQLNWMLELNKWKEERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLSTQPPGLQNFTSINLDNNQLTHFDATNY
                     DRLVKLSLNSNTLESINIHQGRNVSIT"
BASE COUNT          283 a          156 c          118 g          209 t
ORIGIN      
        1 cagaaggtaa ttatataaaa gacaatgact caatgtctat tcgttataga tcaaaatatt
       61 attccagaga ttccgcctta atagaagaag aaacaatatt ttctgacgca gaactaaaag
      121 ctattctgcc tatgcgtcgc atgtacgggg ttagtgacta taagtcaaat tcctcttctc
      181 tatcctcaca ctcgggactc aaggacccaa cgggcacacc cgtctgttat tatattcata
      241 atgagagtaa accttcctta ggttatggtc caacatccaa taattggtta agccaatcct
      301 ttacaacaga gttataaaac acatcctggt ttatcaaggg tgataaatca gaaaaacatt
      361 gtattactat ggagcagtta tgaaattccc ttcaatattt aacaaaataa aaccacaatc
      421 catacagcaa catccagaaa aaaatcaact taactggatg ctcgaattaa ataaatggaa
      481 agaagaacgt atacttacag gtgaaatcca tcgtccggaa tgtcgaaacg aagccgctaa
      541 aaggataaac tgtgcttttt tgtcgaaaca gaatgacatt gatttatcag gacttaattt
      601 atctactcaa ccaccagggc tgcaaaactt cacctctatc aatcttgata ataaccaact
      661 cacacatttt gatgcaacca actacgatag actcgtaaaa cttagtctga atagtaacac
      721 tcttgagtca ataaatattc atcaaggcag aaatgtaagc attaca
//
LOCUS       DAZKIF010000415          742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZKIF010000415 DAZKIF010000000
VERSION     DAZKIF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.419"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>742
                     /locus_tag="ACHOTW_005198"
     CDS             <1..>742
                     /locus_tag="ACHOTW_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847085.1"
                     /translation="SKKVDSYINENKKNLSKEDAGALRDYAKLKMKELFSMLDDDGYK
                     KIITTNAYIKERDKLSGIIYNIENSIISGHESFDIIRSNQHEWGDLSTVEQFKKFEFY
                     VKSELSSAKSIFDDIKNKYITDPETKRNVLYHQLDSDIKERIAFLDISHYAYPGSLLE
                     KLQLSGYVFSDINIIAEYLLASYGVSGHYSHGVVYPAPSDKLLELLRRHTKSNSEWIE
                     KITPYVYDILSDNVSNVLRPPLSEEQKKI"
BASE COUNT          294 a          105 c          127 g          216 t
ORIGIN      
        1 aagtaaaaaa gtagatagtt atataaatga aaacaaaaaa aacttatcta aggaggatgc
       61 cggagcttta agagattatg caaaattaaa aatgaaagaa ctattttcga tgttagatga
      121 tgatggttat aaaaaaatca taactacaaa tgcatatatt aaagagaggg ataaactatc
      181 aggcattata tacaacatcg aaaattcgat tatatcgggt catgaatcat ttgatataat
      241 acgaagcaat caacatgagt ggggggattt atcaacggta gagcaattta aaaaatttga
      301 gttttatgtg aaaagtgaat tatcttcggc taagtcaatt ttcgatgata taaaaaacaa
      361 atatataaca gatcctgaaa caaaacgaaa cgtactctac catcagttag atagtgatat
      421 taaagagcga atagcatttc tcgatatatc ccactatgca tatccgggta gtttattaga
      481 aaaattacaa ttaagtggct acgttttttc tgatatcaac ataattgcag aatacttatt
      541 ggctagttac ggagtttctg gacattattc tcacggcgtg gtatatccag ctccatctga
      601 taaattactt gagctattaa gaagacatac aaagagcaat tctgagtgga tagaaaaaat
      661 aaccccatat gtttatgata tattatccga taatgtatcc aacgtcttgc gccccccttt
      721 atcagaagaa cagaaaaaaa ta
//
LOCUS       DAZKIF010000416          733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZKIF010000416 DAZKIF010000000
VERSION     DAZKIF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.420"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>733
                     /locus_tag="ACHOTW_005199"
     CDS             <1..>733
                     /locus_tag="ACHOTW_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9847086.1"
                     /translation="QLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQETEQNDQ
                     KAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDATYQETF
                     DEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADHSIKVNGHQEITST
                     SRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDT
                     IKWWLKQSREAQSAIMTDEIPLDDAL"
BASE COUNT          240 a          172 c          193 g          128 t
ORIGIN      
        1 atcagttgct cgccgcatca cgtggtgaat ttgttgcagg gattagcgac ccgaatgatc
       61 cgaaatgggt gaaggggatt gaaacccgcg attctgtgaa ccagaaccag caagaaacgg
      121 aacagaacga ccagaaagcg gaacaaaaca gcccaaatac gcaacaaaac gagccagaaa
      181 cgaaacaacc tgagccagta gcgcaacagg aaccggaaaa agtctgcacc gcctgcggtc
      241 agaccggcgg cggcaactgc cctgattgtg gcgcggtgat gggcgacgca acataccagg
      301 aaacattcga tgaagagtat caggttgaag ttcaggaaga tgatccggag gaaatggaag
      361 gcgctgaaca tccacacaag gagaacactg acggcaatca gcatcacgat agcgataatg
      421 aaactggcga gacggcagat cactcaatta aggtgaacgg tcatcaagaa atcacatcca
      481 ccagcaggac gtgtgaccat ctaatgatcg accttgaaac catgggaaaa aatcctgatg
      541 ccccgatcat ctcaataggt gcaatatttt tcgatccgca aaccggagat atgggaccgg
      601 aatttagtaa gactatcgat ctggaaactg ctggcggggt cattgatcgg gacaccatta
      661 aatggtggct taagcaatca cgcgaagcgc aatctgccat tatgaccgat gaaatcccgt
      721 tagatgatgc act
//
LOCUS       DAZKIF010000417          731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZKIF010000417 DAZKIF010000000
VERSION     DAZKIF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.421"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..615
                     /locus_tag="ACHOTW_005200"
     CDS             <1..615
                     /locus_tag="ACHOTW_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9847087.1"
                     /translation="GQLEAWQENAMFDTGRYRRKKIRYRDEVTGKMITRDNPPIPGKQ
                     SLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHKAWLLWCYSGSICWEYQIAITQWAWN
                     EFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAELFGGEGYEYKELALLVGVTTKNWSK
                     TFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKAAFSKQSVAKVD"
BASE COUNT          220 a          136 c          195 g          180 t
ORIGIN      
        1 ggtcagcttg aagcctggca ggagaatgcc atgttcgata cagggcgtta caggcgaaaa
       61 aaaatccggt accgcgatga agtgactgga aaaatgataa cgcgggataa tccaccaatc
      121 ccgggaaagc aatcgctggc gaaggtgacg tcaattcctc tggtcagtcc ggttgagttt
      181 tcgacatcat cgtggcggcg ggctgttctg tctcttgaag aacatcataa agcctggttg
      241 ttgtggtgtt acagcgggag tatttgttgg gaatatcaga tcgcgataac acagtgggcg
      301 tggaatgaat ttaatactca atccggtacc agaaaaattg cagggaaaac gcaggaacgc
      361 ctgaaaaaat taatctggct ggcggcgcag gcagtaaaag cagaactttt tggtggggaa
      421 ggttatgaat acaaggagct ggcattactg gtgggagtga caactaaaaa ctggtccaaa
      481 acatttactc gtcactgggt tgcaatgaaa cacatttttc accgactgga tagtgaggct
      541 ttattgtttg taatgagaac gcgttcaaaa caaaaggcgg cattttcaaa gcaaagtgtt
      601 gcaaaagtag attgaaaggc atatatttca tgcaaatctg atattttgcc gattttgtac
      661 gtgatggcaa aagcaaacaa aacccgccca caagcgggtt tttttgtgcc acttatctcg
      721 gatagacatg g
//
LOCUS       DAZKIF010000418          730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZKIF010000418 DAZKIF010000000
VERSION     DAZKIF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.422"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>730
                     /gene="escU"
                     /locus_tag="ACHOTW_005201"
     CDS             <1..>730
                     /gene="escU"
                     /locus_tag="ACHOTW_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFP9847088.1"
                     /translation="AIREILGAVLNIFLLYILPISLIVFVGTVTTGVSQIGFIFAVEK
                     IKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIIVLIFYFMGHSYANEFANFTGLN
                     AYQALVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKMKMSKDEVKREAKDTDG
                     NPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICLYYKLGETPLPLVIETGK
                     DAKALQIIKLAELYDIPVIEDIPLA"
BASE COUNT          230 a          113 c          141 g          246 t
ORIGIN      
        1 atgccattcg agaaatatta ggtgcggtat taaatatatt tttattatat attttgccaa
       61 tttctttgat tgtctttgtt ggaactgtta cgactggtgt atcacaaata ggattcatct
      121 ttgcggttga aaaaataaaa ccatcggctc agaagattag tgtaaaaaat aacctgaaaa
      181 atattttttc tgtaaagagc atttttgagc tacttaaatc agtatttaag ttagtgataa
      241 ttgttctcat tttttatttt atggggcatt catatgcaaa tgagtttgct aatttcacag
      301 gactgaacgc atatcaagct cttgtcgttg ttgccttttt tgtttttctt ttatggaaag
      361 gcgtgctatt cggatatcta ctcttttcag tatttgattt ctggttccag aagcatgagg
      421 gactgaagaa aatgaaaatg agtaaagatg aggtgaaacg agaagccaag gatactgatg
      481 gtaaccctga aattaaaggg gagcgccgtc gccttcattc cgagatacaa agtggaagtt
      541 tggctaataa catcaaaaaa tcaaccgtta ttgttaaaaa cccgactcac attgcgattt
      601 gcctatacta taaacttggg gagactccat tacctttagt tattgaaaca ggaaaagatg
      661 ccaaagctct acagatcatt aaactggctg aactctatga tattccagtg attgaagata
      721 ttcctttagc
//
LOCUS       DAZKIF010000419          729 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZKIF010000419 DAZKIF010000000
VERSION     DAZKIF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.423"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            95..691
                     /gene="fimE"
                     /locus_tag="ACHOTW_005202"
     CDS             95..691
                     /gene="fimE"
                     /locus_tag="ACHOTW_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP9847089.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          199 a          138 c          175 g          217 t
ORIGIN      
        1 gtggttatct ttttatctat tgggctaatt ttgaccgatt gaggtttcct ataggtattc
       61 attcaaatat atctcagtta ggagtactac tattgtgagt aaacgtcgtt atcttaccgg
      121 taaagaagtt caggccatga tgcaggcggt ttgttacggg gcaacgggag ccagagatta
      181 ttgtcttatt ctgttggcat atcggcatgg gatgcgtatt agtgaactgc ttgatctgca
      241 ttatcaggat cttgacctta atgaaggccg aataaatatt cgccgactga agaacggatt
      301 ttctaccgtt cacccgttac gttttgatga gcgtgaagct gtggaacgct ggaccctaga
      361 acgtgctaac tggaaaggcg ctgaccggac cgacgccata tttatttctc gccgcgggag
      421 tcggctttct cgccagcagg cctatcgcat tattcgtgat gccggtattg aagctggaac
      481 cgtaacgcag actcatcctc atatgttaag gcatgcttgc ggttatgaac tggcggagcg
      541 tggtgcagat actcgtttaa ttcaggatta tctcgggcat cgaaatattc gccatactgt
      601 acgttatacc gccagtaatg ctgctcgttt tgccggatta tgggaaagaa ataatctcat
      661 aaacgaaaaa ttaaaaagag aagaagtttg atttaactta ttgataataa agttaaaaaa
      721 caaataaat
//
LOCUS       DAZKIF010000420          714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZKIF010000420 DAZKIF010000000
VERSION     DAZKIF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.424"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..232
                     /locus_tag="ACHOTW_005203"
     CDS             <1..232
                     /locus_tag="ACHOTW_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFP9847090.1"
                     /translation="DLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEARG
                     AWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            189..635
                     /locus_tag="ACHOTW_005204"
     CDS             189..635
                     /locus_tag="ACHOTW_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP9847091.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPRRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            632..>714
                     /locus_tag="ACHOTW_005205"
     CDS             632..>714
                     /locus_tag="ACHOTW_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP9847092.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDI"
BASE COUNT          193 a          170 c          205 g          146 t
ORIGIN      
        1 cgacctatca ttaacccagc caaagctaaa agaatgtccg ttttgcggcg gtaatgctcg
       61 tctgtgggtt gaggccggaa taaatattga tgtgtggggc tatgcagaat gtgacctctg
      121 tgaagccagg ggggcatggg caccatcagt tgctgcggcg gctgaaaaat ggaaccggag
      181 agcaggagat gaagcaaacc tttctgcttc gcaacgaagc aatcagaaat aacgccatag
      241 acgccattct ctcactaccc atcgacgaca agtcacccca cgaagtccac gttaaagaac
      301 ccaggcgcag caaagcgcag aatgaccgta tgtggccgat gctgaacgat gtttcgcgtc
      361 aggtgctatg gcatggtcaa cggctggcgc cggaagactg gaaagacctg ttcactgccc
      421 tgtggcttaa gaccaaaaaa ctggagcaac gaagtgtgcc tggtatcgac ggtggcgttg
      481 tcatgcttgg cgtgcgtacc agcaaaatgc ggaaggccag catgactgag cttatcgaaa
      541 tcatgttctg gttcggctca gagcgcaacg tgcggtggag tgatgactcc cggcgagagt
      601 atgaatggtc acaacgaaaa ggtagggctg catgactatc aaatcaaata cgccagcaca
      661 cgacaaggac tgctggcaaa cgccgctttg gctttttgat gcactggata ttga
//
LOCUS       DAZKIF010000421          710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZKIF010000421 DAZKIF010000000
VERSION     DAZKIF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.425"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>710
                     /locus_tag="ACHOTW_005206"
     CDS             <1..>710
                     /locus_tag="ACHOTW_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9847093.1"
                     /translation="KGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTTSQ
                     FLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQMTVNYHIR"
BASE COUNT          165 a          185 c          227 g          133 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacgccggtt gaggcaaagg acaatcttaa gtccacgcag
       61 atgatgagcg tgattgatgc cattggtgaa gggccgattg aaggtccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggtaatcc tgtgatacat
      181 ggtgtgaccg cggtctggcg cgccggggag caggagcaga caccacctga aggctttgag
      241 tcctccgggg cggaaaccgc actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacgt ccgcgaacat tgaccgcctg cgggtcacct tcggggtgca gtcactgttg
      361 gagaccacct caaagggcga ccgtaatccc tcttctgtcc gactgctgat tcagttgcag
      421 cgtaacggta actgggtgac ggaaaaggat gtcaccatta acggcaagac cacctcgcag
      481 tttctggcgt cggtgattct ggataatctg cctccccgtc cttttaacat ccggatggtc
      541 cgggagacgg cggacagcac ctcggaccag ctgcagaata agacgctctg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgattgtggg gctgcaggtg
      661 gatgcggagc agtttggtgg ccagcagatg acggtgaact accatatccg
//
LOCUS       DAZKIF010000422          705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZKIF010000422 DAZKIF010000000
VERSION     DAZKIF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.426"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..64
                     /locus_tag="ACHOTW_005207"
     CDS             <1..64
                     /locus_tag="ACHOTW_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFP9847094.1"
                     /translation="PKYAIKLQFVYTLLTKNRVL"
     gene            67..516
                     /locus_tag="ACHOTW_005208"
     CDS             67..516
                     /locus_tag="ACHOTW_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFP9847095.1"
                     /translation="MRKITIILLLTFSCTKGLAFDISMSKECSGVDELEFIDCVKNSF
                     IMSTITLKKAEANIKEKIQKWNSDEDEAIRKEAVISLKRLESSNREFIRYRFAQCSFT
                     LTWGARVGRLAFSRLYPCYTELNKIRAMQLNNAYISDFQNPDEISSK"
BASE COUNT          254 a          117 c          115 g          219 t
ORIGIN      
        1 tcccaagtat gcaataaaac ttcagttcgt ttatacatta ctcacaaaaa acagagtatt
       61 atgagaatgc gaaaaataac aattattcta ctgctgacat tctcatgtac aaaaggtctg
      121 gcatttgaca tttccatgtc taaagaatgt agtggtgttg atgaacttga atttattgat
      181 tgtgttaaaa actcatttat tatgagcact attacactta aaaaagcaga agcaaatata
      241 aaagagaaaa ttcaaaaatg gaatagtgat gaggacgaag caataagaaa agaagcagtt
      301 atttctttaa aaagactaga gtcttctaat agggaattta tacgttacag atttgctcaa
      361 tgctctttta ctctaacctg gggggccaga gtagggcgac tggcattctc tcggctatat
      421 ccatgctata ctgagttaaa caaaataaga gccatgcaac taaacaatgc ttacatttct
      481 gactttcaaa atccagatga gatatccagc aaatgattat tataaattta agattaacat
      541 tactatttat actggccaca tttacatatt cagtacaatc agacgaattc gatgatcaaa
      601 ccacttacac gcaataccgt tatttttcca ctgctatacc ggaagatata cgtagattta
      661 tgttctacgt agattattgc tttgtagatt tagcattcga tatcg
//
LOCUS       DAZKIF010000423          703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZKIF010000423 DAZKIF010000000
VERSION     DAZKIF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.427"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            36..>703
                     /locus_tag="ACHOTW_005209"
     CDS             36..>703
                     /locus_tag="ACHOTW_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9847096.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDAL"
BASE COUNT          230 a          122 c          156 g          195 t
ORIGIN      
        1 agtaatgtaa ataaattttc gaggagatca ttcctgtggg acgtaaatgg gccaatattg
       61 ttgctaaaaa aacggctaaa gacggtgcaa cgtctaaaat ttatgcaaaa ttcggtgtag
      121 aaatctatgc tgccgctaaa caaggtgaac ccgatccaga attaaacaca tctttaaaat
      181 tcgttattga acgtgcaaag caggcacaag ttccaaagca cgttattgat aaagcaattg
      241 ataaagccaa aggcggcgga gatgaaacat tcgtgcaggg ccgttatgaa ggctttggtc
      301 ctaatggctc aatgattatc gccgagacat tgacttcaaa tgttaaccgt acgattgcta
      361 acgttcgcac aattttcaat aaaaaaggcg gcaatatcgg agcggcaggt tctgtcagct
      421 atatgtttga caatactggt gtgattgtat ttaaaggatc agaccctgac catatttttg
      481 aaattttact tgaagctgaa gttgatgttc gtgatgtgac tgaagaagaa ggtaacattg
      541 ttatttatac cgaacctact gaccttcata aaggaatcgc ggctctaaaa gcagctggaa
      601 tcagtgagtt ctcaacaaca gaattagaaa tgattgctca atctgaagtt gagctttcac
      661 cagaagattt agaaatcttt gaagggcttg ttgatgccct tga
//
LOCUS       DAZKIF010000424          699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZKIF010000424 DAZKIF010000000
VERSION     DAZKIF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.428"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          243 a          119 c          130 g          207 t
ORIGIN      
        1 ttcctctgaa acagagaggt tcaagataaa gtatcaacaa gagaatacac ttaatgatgt
       61 agttcaaaat atcaggactt ggctaaaaga aaatcaaaat atggtgaaag ctattaagaa
      121 aattgatact gaataacatg gcgaataaat aaacggatca agtcgttcct atgacaatga
      181 ggttatacga aaagacgctt actcatcaga aatctggtat gctttaatta aaccagtgtc
      241 gctgatgtgt aagcgttgac gttgggagac gattaaatgg ttataccact cctacgtctt
      301 ttccatacgg cgtataagag tgcaacatgt tgagttttcc gagtgaatgg tgcattttac
      361 taaccgagcc atttgaatca accaacgcaa taaaaaagac tggaataata agcctgtatt
      421 tattatttat cgcaatcgca ctttacggtt ttatccaact gaaaagagac tgcttccagc
      481 ggttgtggat tatccctggg cgatagtcat agtctggtgt caggattcac aaatgacctg
      541 tttccttaca tggatataaa cacaacatag agtgcggtag ttaatctgaa taatctaatt
      601 tcggctattc agcacaaaac aacattattg attctttata agagagaatc tctccatcat
      661 ttttatataa atcattatta agaaaattca gaagctctt
//
LOCUS       DAZKIF010000425          693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZKIF010000425 DAZKIF010000000
VERSION     DAZKIF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.429"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>693)
                     /gene="wzx"
                     /locus_tag="ACHOTW_005210"
     CDS             complement(<1..>693)
                     /gene="wzx"
                     /locus_tag="ACHOTW_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFP9847097.1"
                     /translation="HAGLPVLIVSTLGIQYISGIYLTINLIIKRLIKFTKVNIHAKRE
                     APYLILNGFFFFILQLGTLATWSGDNFIISITLGVTYVAVFSITQRLFQISTVPLTIY
                     NIPLWAAYADAHARNDTQFIKKTLRTSLKIVGISSFLLAFILVVFGSEVVNIWTEGKI
                     QVPRTFIIAYALWSVIDAFSNTFASFLNGLNIVKQQMLAVVTLILIAIPAKYIIVSHF
                     GLTVMLYCFIFI"
BASE COUNT          256 a          117 c          108 g          212 t
ORIGIN      
        1 atataaaaat gaagcagtac aacataacag ttaacccaaa atggctaact atgatgtatt
       61 ttgctggaat tgcgatcaat atcaatgtta caacagcaag catttgttgt ttaactatgt
      121 tcaaaccatt taaaaagctt gcaaatgtat tcgaaaaagc atcaataaca gaccataaag
      181 cataagctat tatgaatgtt cgaggtacct gaatctttcc ttctgtccaa atattaacga
      241 cttcactacc gaacactact aatatgaagg ccaataagaa tgatgaaata cccactattt
      301 tcaatgatgt tctgagcgtc ttttttataa attgagtatc attgcgtgca tgagcatctg
      361 cataagcagc ccataacggg atgttataaa tcgtaagagg gaccgtagat atttgaaata
      421 atctctgtgt aatgctaaaa acagcaacat aagtaacacc caatgttata gatattataa
      481 agttatcacc actccatgtt gccagagtgc ctaactgtaa aataaaaaag aaaaaaccgt
      541 ttaatatcaa atatggagct tctcttttag catgtatgtt aacttttgta aactttatta
      601 atcgctttat aataagatta attgttaaat agattcccga tatgtattga ataccaagag
      661 tgctgacaat taaaactggt agtcccgcat gta
//
LOCUS       DAZKIF010000426          693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZKIF010000426 DAZKIF010000000
VERSION     DAZKIF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.430"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>693
                     /locus_tag="ACHOTW_005211"
     CDS             <1..>693
                     /locus_tag="ACHOTW_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP9847098.1"
                     /translation="MGPKGEKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPR
                     GEPGPAGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKA
                     ATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGDKGDTGPAGPAGPKGDKGERGDTG
                     PVGATGERGPAGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQGP
                     KGETGAAGPVGAT"
BASE COUNT          179 a          164 c          282 g           68 t
ORIGIN      
        1 tatggggcca aaaggcgaga aaggtgatcc gggaggtcct gcaggcccgc aaggtcctaa
       61 aggcgaacga ggagaagccg gaccacaggg accgatggga gcacgaggtg agcgtgggga
      121 gactggcccc cgaggtgaac ctggtcctgc aggtccgaga ggcgaacgag gagagaccgg
      181 acctcaggga cctcgtggag agccaggtcc ggcaggcagc gctgcaaatg tggctgatgc
      241 aacgacggca cagaagggaa ttgtgcagtt aagcagcgca acggacagtg atgatgaaac
      301 gaaggctgcc accccgaaag cggtgaaagc ggcaatggat gtggcaaatg aagcgaaaac
      361 aaaggcagaa gaggctgcag caggaggtgg tgttcccggt ccgaaaggag ataaagggga
      421 cacggggcca gcaggtccgg ctgggccgaa gggtgataag ggagagcgcg gtgacaccgg
      481 ccctgtcggg gcaaccggcg aacggggacc ggcaggtgat gctggtccgg caggcccgca
      541 ggggccgaaa ggtgacaggg gagagcgggg agagaccggt ctgacgggaa atgcaggtcc
      601 acagggtcca aagggagata ccggtgcggc aggcccggca ggcccacagg gaccgaaagg
      661 agaaacaggt gcggcaggcc cggtgggggc gac
//
LOCUS       DAZKIF010000427          685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZKIF010000427 DAZKIF010000000
VERSION     DAZKIF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.431"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            24..167
                     /gene="kil"
                     /locus_tag="ACHOTW_005212"
     CDS             24..167
                     /gene="kil"
                     /locus_tag="ACHOTW_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFP9847099.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILMLKLRSSK
                     SIH"
     gene            243..539
                     /locus_tag="ACHOTW_005213"
     CDS             243..539
                     /locus_tag="ACHOTW_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9847100.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            545..>685
                     /locus_tag="ACHOTW_005214"
     CDS             545..>685
                     /locus_tag="ACHOTW_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9847101.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IAL"
BASE COUNT          194 a          166 c          164 g          161 t
ORIGIN      
        1 taatcagcca ggagtcccaa agaatggatc aaacacttat ggctatccag actaaattca
       61 ctatcgccac ttttattggc gatgaaaaga tgtttcgtga agctgtcgac gcttataaaa
      121 aatggatatt aatgctgaaa ctgagatcaa gcaaaagcat tcactaaccc catttcctgt
      181 tttcctaatc agcctggcat ttcgcgggcg atattttcac agccattttc aggagttcag
      241 ccatgaacgc ttattacatt caggatcgtc ttgaggctca gagctgggcg cgtcactacc
      301 agcagatcgc ccgtgaagag aaagaggcag aactggcaga cgacatggaa aaaggcctgc
      361 cccagcacct gtttgaatcg ctatgcatcg atcatttgca acgccacggg gccagcaaaa
      421 aagccattac ccgtgcgttt gatgacgatg ttgagtttca ggaacgcatg gcagaacaca
      481 tccggtacat ggttgaaacc attgctcacc accaggttga tattgattca gaggtataaa
      541 acggatgagt acagcactcg caacgctggc tgggaagctg gctgaacgtg tcggcatgga
      601 ttctgtcgac ccacaggaac tgatcaccac tcttcgccag acggcattta aaggtgatgc
      661 cagcgatgcg cagttcatcg cattg
//
LOCUS       DAZKIF010000428          683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZKIF010000428 DAZKIF010000000
VERSION     DAZKIF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.432"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..277
                     /gene="ler"
                     /locus_tag="ACHOTW_005215"
     CDS             <1..277
                     /gene="ler"
                     /locus_tag="ACHOTW_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFP9847102.1"
                     /translation="QEVQSVIESIQKQITYYNITLQELGYTNVPDDGLARRNSSKGVY
                     YRNEEGQTWSGVGRQPRWLKEALLNGMKKEDFLVKDTEEEIIPLKNI"
     gene            292..510
                     /gene="escE"
                     /locus_tag="ACHOTW_005216"
     CDS             292..510
                     /gene="escE"
                     /locus_tag="ACHOTW_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFP9847103.1"
                     /translation="MITITELEDEIIKNKEAANVFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRTIEIMRIRINNK"
     gene            523..>683
                     /gene="cesAB"
                     /locus_tag="ACHOTW_005217"
     CDS             523..>683
                     /gene="cesAB"
                     /locus_tag="ACHOTW_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesAB"
                     /protein_id="HFP9847104.1"
                     /translation="MSQTRNKELLDKKIRSEIEAIKKIIAEFDVVKESVNELSEKAKT
                     DPQAAEKLN"
BASE COUNT          290 a           96 c          148 g          149 t
ORIGIN      
        1 gcaggaagtt caaagtgtaa ttgagtcgat tcagaagcag attacttatt acaatataac
       61 cttacaagag ctgggatata ctaatgtgcc tgatgatgga ctcgctcgcc ggaactcatc
      121 gaaaggtgtt tactaccgca atgaagaagg gcagacctgg tcgggcgtag gccgacagcc
      181 acgctggctt aaagaagcac tgttgaatgg aatgaagaaa gaagattttc ttgtgaagga
      241 cactgaagaa gaaataatac cgctgaaaaa tatttaacat gaaataatta aatgataacg
      301 ataactgagc tggaagatga aataataaaa aataaagaag ccgcaaatgt ttttattgaa
      361 aaaataaacg acaaaaagaa cgaaatccat gaaaaaatga aacacccttt ggataaagtt
      421 acctacaatg aagcaaagga actgctcatt gcgtgtgatg cggcaattag gactatagag
      481 ataatgcgaa tcaggattaa taataaatag aggatgagta ttgtgagcca aacaagaaat
      541 aaagaactat tagataaaaa aataagatca gaaattgagg cgataaaaaa aataatcgcc
      601 gaatttgatg tcgtaaaaga aagcgttaat gagttaagtg aaaaagcaaa aacagatcct
      661 caggcggcag aaaaattaaa caa
//
LOCUS       DAZKIF010000429          671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZKIF010000429 DAZKIF010000000
VERSION     DAZKIF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.433"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>671)
                     /locus_tag="ACHOTW_005218"
     CDS             complement(<1..>671)
                     /locus_tag="ACHOTW_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFP9847105.1"
                     /translation="TLIKQINDLKMCVNEYIGDCTDEELNDREFIASVVDRAIFHFAI
                     NSICNPEDNKDATLIERCTFDVETKNGLPSTVQLFYEESKDNEPLANIHLQAIGSGFL
                     TFVNACQEHDDNSLKLFASLLISLSYSSVYSDFAGRVNINEYNDNYLKAQFEELSQRD
                     MKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLAPDILSKMRDAAPKLIDFAKSFD
                     PNSKE"
BASE COUNT          205 a          142 c          105 g          219 t
ORIGIN      
        1 ttttcctttg agtttgggtc aaacgactta gcaaagtcaa ttaatttcgg tgcagcatct
       61 cgcattttgc ttaaaatatc aggggcgagc gttccttcat tcaccagatg tgacatctta
      121 tccagatagc catcaaaatt catttctccc ccatctgcca gacgcttcat ctctcccagg
      181 tacttcttca tatcacgttg agataattct tcaaactgag ctttcaggta gttatcatta
      241 tattcattaa tattcactct tcctgcaaaa tctgagtaga cactagaata tgaaagggaa
      301 attaacagcg aagcaaataa ttttaagctg ttgtcatcat gttcctggca ggcattaaca
      361 aacgttaaga aaccagaacc tattgcttgc aaatgtatat tcgctaaagg ttcattatct
      421 ttagattcct cataaaatag ctgaaccgtg gagggaagac cattcttggt ttctacatca
      481 aaagtacatc gttctatgag tgtcgcatct ttattatcct cgggattaca tatactatta
      541 atcgcgaaat gaaaaatagc cctgtcaaca actgatgcaa taaattcacg atcgtttaat
      601 tcctcatcag tacaatctcc aatatactca ttgacacaca ttttgagatc gtttatttgc
      661 ttaattaacg t
//
LOCUS       DAZKIF010000430          667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZKIF010000430 DAZKIF010000000
VERSION     DAZKIF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.434"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          222 a          142 c          133 g          170 t
ORIGIN      
        1 ataaacaaat aaaacagaat aaaaaccaag ctgaaccgtt tcactatact ctacttaatt
       61 cgagttacag gaaggcggca agcgagtgaa tccccaggag cttacataag taagtgattg
      121 gggtaagcga acgcagtcgc agcacatgca acttgaagta tgacgagtat atatatctat
      181 tagctgtttc ccggacataa gctacagcca gcactaacct ggaacgggtg cattataaaa
      241 aggaacaagg gacaaatagc tgaacagtaa ccgcgttagt ctcttcccgc ataaccgtac
      301 cattcgtatt aagaagtatt gcccatatcc attcggcgaa agaggatctg ccatacatca
      361 agtccgtttt acttggcatc agatcatcgc cgtcattaac tctgttaaag tcggacaaac
      421 gattagcaag atctgtgatt caaaagtgct tagcgctaag gtgggactgg ttacagattc
      481 attaaccctg ttctttgtcg cacctatcga tcggtgtaat gccacaacag gtttatgtta
      541 atcagaacaa tgcgcccctc tataaagaga attctttgat aaggcaactt gaaaagggaa
      601 agctaactga tatcaatgta ccactttagt aatcatcaca ttatatacag gccaaaataa
      661 gtttcct
//
LOCUS       DAZKIF010000431          667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZKIF010000431 DAZKIF010000000
VERSION     DAZKIF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.435"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>667
                     /locus_tag="ACHOTW_005219"
     CDS             <1..>667
                     /locus_tag="ACHOTW_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847106.1"
                     /translation="LSTSTDSIANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFV
                     EHVITYKNEFAADYEPYTLTFTRGENNEGILSVESKEGFISQRTINLNEYETAINIIN
                     EHVIKENIHNTVQSPTEKYISKINSSDKPYKTSSKESIKSQLYSDQRNIQTCYYILRK
                     NTEWYKYASSEERYDKFKNSSKEIKNTYKQIVQAQKKLNQMKYINKLSGELIDITDKK
                     LAP"
BASE COUNT          278 a          123 c          102 g          164 t
ORIGIN      
        1 ggttatcaac ctcaacagac agcatagcta atttattggc ttttttcacg aaagcctcag
       61 atgcaattga tagtatcaag acccaaaaaa ttgacgttca cagttatggt ttcgtaccaa
      121 taagacattt tgtcgaacat gtcataacgt ataaaaatga atttgcagcg gattatgaac
      181 cctataccct tacctttacc cgaggtgaaa acaacgaagg tattttgagt gtcgaaagca
      241 aagaagggtt tatttcacaa agaacaataa acttaaatga atatgaaact gcaattaaca
      301 tcatcaatga acatgttata aaagaaaata tccacaacac ggtccagtca ccgacagaaa
      361 aatacatttc aaaaataaac agctctgata aaccttataa aaccagcagt aaggaaagta
      421 ttaaaagtca actttacagc gatcaaagaa atatacagac ctgctactac attctgagaa
      481 aaaatacaga atggtacaaa tacgcatcct ccgaggaacg ctatgataaa ttcaagaatt
      541 cttctaagga aattaaaaat acctacaagc aaattgtaca agcgcagaaa aaattgaacc
      601 aaatgaaata catcaataaa ttaagtggtg agcttatcga tatcaccgac aaaaagttag
      661 caccatt
//
LOCUS       DAZKIF010000432          652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZKIF010000432 DAZKIF010000000
VERSION     DAZKIF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.436"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            85..510
                     /locus_tag="ACHOTW_005220"
     CDS             85..510
                     /locus_tag="ACHOTW_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9847107.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            507..581
                     /locus_tag="ACHOTW_005221"
                     /pseudo
     CDS             507..581
                     /locus_tag="ACHOTW_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          185 a          160 c          161 g          146 t
ORIGIN      
        1 taagcgtaaa ctgaccgccg tatgtagcca tcagacgaaa attggtaact tagacgccca
       61 tctgatatag acggacatct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
      121 aactattcga atgaattcaa acttcgtatg gtggaactgg catcacaacc aggagcctct
      181 gttgcccgta ttgcccggga acacgatatc aatgataacc tcctgttcaa atggctcagg
      241 ctctggcaga acgaagggcg tatatcacgg cgtcttcagg taacgacctc ttctgacact
      301 ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaagaacc ggtggcggcc
      361 attgcgccgt ctttatccac ttccactcag accagtgtca gtgccggctc ctgcaaggtg
      421 gagttccgtc acggtaacat gacgctggaa aatccatcgc cagagctgct cacgctgttg
      481 atccgtgaac tgaccgggag gggacgatga tctcactccc gtcaggtacc cgcatctggc
      541 tcgttgccga cgttaccgat atgcgtaaat ccttcaacgg atacgacata tgctgaagga
      601 ggagcagtaa tggattaagt gataacaaat acccagaaat agaggttcaa at
//
LOCUS       DAZKIF010000433          649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZKIF010000433 DAZKIF010000000
VERSION     DAZKIF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.437"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..630
                     /gene="espJ"
                     /locus_tag="ACHOTW_005222"
     CDS             <1..630
                     /gene="espJ"
                     /locus_tag="ACHOTW_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     ADP-ribosyltransferase EspJ"
                     /protein_id="HFP9847108.1"
                     /translation="SLINNALNIQKTSYSLTKMEQAGKLLNRKITPENTPPMLLSYRN
                     ADLTQEKNITERVLSIFKIKRDFVAVRIQNNQFTDLKNKKIQGHQNTVASVMDWYNPQ
                     KNALGITMGTPRKSADIAKEEHRNALNFMIMEKNTFHEKILNSNDNLQKSYSKTEDSS
                     WVAASVGSLLDKGAKVYPDTSCSLRLGEPFIFTLPESVRVDVDIYPLKK"
BASE COUNT          267 a          113 c          105 g          164 t
ORIGIN      
        1 tcattaatta acaatgcatt aaatatacaa aaaacatcat attctctgac caaaatggag
       61 caggcaggga agttgttaaa tagaaaaata actccagaaa atactcctcc aatgttgtta
      121 tcatacagaa atgcagattt aacgcaagaa aaaaacatta cagaaagagt tttatccata
      181 tttaaaataa aacgagattt tgttgctgta agaatccaaa acaatcagtt tactgatttg
      241 aaaaacaaaa aaattcaagg ccatcaaaac acagttgcca gtgtcatgga ttggtataat
      301 ccccaaaaaa atgctttggg tataactatg ggtacaccaa gaaaatcggc cgatatagca
      361 aaagaagagc acagaaatgc actaaatttc atgataatgg aaaaaaacac atttcatgaa
      421 aaaatcttaa acagcaatga caatttgcaa aaatcataca gcaaaacaga agacagtagt
      481 tgggtcgcag cttctgtcgg ctctcttcta gataaaggag caaaagtata tcccgataca
      541 tcatgctctc tgagactggg agaaccattc atctttacgc tgccagaatc agttagagtc
      601 gatgttgaca tatatcctct caaaaaataa tcttaaaggt attgacata
//
LOCUS       DAZKIF010000434          646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZKIF010000434 DAZKIF010000000
VERSION     DAZKIF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.438"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            71..>646
                     /locus_tag="ACHOTW_005223"
     CDS             71..>646
                     /locus_tag="ACHOTW_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9847109.1"
                     /translation="MTENTTPTADFSPSKRFFVSMLTRDIDLNDAILDLLDNCVDGAL
                     RTIKDTKKTSKPYEGFYAKLTINKDVFIIEDNCGGIPKSFREYAFKMGRPHQKEEENE
                     GTVGVYGIGMKRAIFKMGRDCSIQSNNPDGAFTVDITPDWIDGDGWKIPMHESDYDNK
                     NPTGTTIEIKKLHSNVAQKFNESTYLTDLFLQ"
BASE COUNT          240 a          111 c          122 g          173 t
ORIGIN      
        1 atgcgaagcg tttaaaatca acaatttgca cttaaggtat ttattttccc aaacacatag
       61 agagtaaatt atgactgaga acacgactcc aacagcagac tttagtccta gtaaaagatt
      121 ttttgtaagt atgctaactc gagatatcga cttaaacgat gccatacttg atctgcttga
      181 taactgcgtc gatggggctt tacgtacaat aaaagacaca aaaaaaactt caaaaccata
      241 tgaaggattt tatgcgaaac ttacgattaa taaagatgtc tttattatag aagacaattg
      301 cggaggtatc cctaaaagct tccgagaata tgcttttaaa atgggacgac cccatcaaaa
      361 agaagaagaa aatgaaggca ccgttggcgt ctatggtatt ggtatgaaaa gagccatttt
      421 taaaatgggt agagattgct caatacaatc gaataatccc gatggcgcat tcactgtaga
      481 tataacacct gattggatcg atggtgatgg ttggaaaata ccgatgcacg agagtgacta
      541 tgataacaaa aatccaacag gcacaactat agagataaaa aaactccatt ctaatgtggc
      601 acaaaaattt aatgagagca catatcttac tgatctcttt ttacag
//
LOCUS       DAZKIF010000435          645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZKIF010000435 DAZKIF010000000
VERSION     DAZKIF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.439"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>645)
                     /gene="espX2"
                     /locus_tag="ACHOTW_005224"
     CDS             complement(<1..>645)
                     /gene="espX2"
                     /locus_tag="ACHOTW_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFP9847110.1"
                     /translation="NKILRMKIELTEGSGFSKSLSDSQIEMHILRTVNSLNVGEKLND
                     DNLADHSIFTNEFSVIIPPSYYDATSAVNANNIVREKLFESDSKVKDIVDDMSNHDVE
                     SEKDIFVIGGMIEKLNSLADESFNDSTDNIQTVKDLLTQLTDGMELFALRDIVAFPST
                     IIAKLIKSPLNSDHELVMRALDTYLCYFRNKNLNNNAEIINFFHALFLKRPELM"
BASE COUNT          191 a          125 c          104 g          225 t
ORIGIN      
        1 ccatcaactc tggtctcttt aaaaagaggg catggaaaaa gttaattatt tccgcattgt
       61 tattcagatt tttatttctg aagtagcaca gatacgtatc cagtgcgcgc ataacaagtt
      121 catgatcgct attaagtggg gattttatta attttgctat tatggttgat gggaatgcca
      181 ctatgtcccg cagcgcgaag agctccatgc catcagttaa ttgcgttaac agatctttta
      241 ctgtttgtat gttatcagta ctatcattga aagattcgtc tgccagagaa ttcagttttt
      301 ctatcatccc cccgattaca aatatatctt tttcgctctc cacgtcatga ttagacatat
      361 catcgacgat atctttaact ttactatcac tttcaaatag tttttctctc acaatattat
      421 tagcgtttac tgcagaagtt gcatcataat aggatggcgg aataataaca gagaattcgt
      481 tagtgaatat agaatgatct gcaagattat catcattcaa tttctcgcca acatttagcg
      541 aattaacagt cctcagaata tgcatctcaa tctgactgtc agaaagactt ttagaaaaac
      601 cactaccttc agtcagttca attttcatcc tgagtatttt atttt
//
LOCUS       DAZKIF010000436          642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZKIF010000436 DAZKIF010000000
VERSION     DAZKIF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.440"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..161)
                     /locus_tag="ACHOTW_005225"
     CDS             complement(<1..161)
                     /locus_tag="ACHOTW_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9847111.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALA"
     gene            complement(142..567)
                     /locus_tag="ACHOTW_005226"
     CDS             complement(142..567)
                     /locus_tag="ACHOTW_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9847112.1"
                     /translation="MAREMGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFS
                     GLMYAVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGD
                     GGRDISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
BASE COUNT          143 a          211 c          145 g          143 t
ORIGIN      
        1 gccagcgcct gtcggctcag cccctgttca acagtactgg cggtttttct ggcatccgcc
       61 tccagactgg aaaaatgacg gcgtacccgg gccatctgtt catcaaaccg ggccgcatcc
      121 agactcaaat caataaccag atcacccgct ggctgggaca tatctcacac ctccggaaat
      181 ccccgctgaa gccatcatta atgcaacatc atcctcgctg acatccacca catccgcaga
      241 aggtgaaata tcgcgccctc cgtcccctcc gaaccggacg cctccggcaa ctcctgtcgc
      301 tttctgcatc agcatttctt cctcgtccgg catctccgtc tgctcttcct cacacgctgg
      361 agcaagcaga ctgaaatccg ccggatgcat atccggatcg ccaaaaaaca ggctgagtac
      421 ggcgtacatc agcccggaaa aatgagcgtc cagttgggta tcctgaaaat aatgcgtgca
      481 gtaaaaacgt cgccagtcgg catattcggt ggatgtcatc ccggcaagca tggcgcgcca
      541 gtcgggtctc cccatctctc gcgccagttt caggacaaag ttcagctcgc cttcgaatgc
      601 ttttttgatg ttaccgactc agtcgcttct gctttaccgg tt
//
LOCUS       DAZKIF010000437          633 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZKIF010000437 DAZKIF010000000
VERSION     DAZKIF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.441"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..194)
                     /locus_tag="ACHOTW_005227"
     CDS             complement(<1..194)
                     /locus_tag="ACHOTW_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847113.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALA"
     gene            complement(244..546)
                     /locus_tag="ACHOTW_005228"
                     /pseudo
     CDS             complement(244..546)
                     /locus_tag="ACHOTW_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
BASE COUNT          138 a          204 c          141 g          150 t
ORIGIN      
        1 gccagcgcct gtcggctcag actctgctca gcagcatcag cctgttttct gaaagactga
       61 ctgactttcc cggacatcaa atcagcaagc gcactggttt cacccagctc tttttttacc
      121 cgcgctgcct cttcagaaaa acgggcagaa tccagtgtca gcacggctgt cagatcggca
      181 aaattacctg ccatcgtggt cacctcctga aatgtcctct gataccatca ataactgtca
      241 caacctcctt ccctcctccc cgaaacggac tccaccggcg aggcccgccg ccttctgcat
      301 cagtacatca ttttcgtccg gcgtctccgc ctgtccttcc tctgcctccg gagcgaacag
      361 gctgaaatcc gccggatgca tatccggatc gcaaaaaaac aggctgagta cggcgtacat
      421 cagcccggaa aaatgcatat ccagctgggt atcctgaaaa taatgcgtgc agtaaaaacg
      481 tcgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcggctct
      541 ccccatctca cgcgccagtt tcaggacaaa gttcagctcg ccttcgaatg cttttttgat
      601 gttaccggct cagtcgcttc tgttttcccg gtt
//
LOCUS       DAZKIF010000438          632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZKIF010000438 DAZKIF010000000
VERSION     DAZKIF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.442"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..317
                     /locus_tag="ACHOTW_005229"
     CDS             <1..317
                     /locus_tag="ACHOTW_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847114.1"
                     /translation="WHDGVLVETRMECHHTEYNFILTAELYVDEFTAGRERKRFEFTG
                     VEHLSQVMDIAELIDNQNAGNIKDATIVYKAKKYKISLHLFGGFLSFTFKTMRVIDNE
                     CA"
     gene            304..>632
                     /locus_tag="ACHOTW_005230"
     CDS             304..>632
                     /locus_tag="ACHOTW_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847115.1"
                     /translation="MNVRNVKFDNLSFYEEKIRWRMRRQISNAEAIFYENQEEHIRRF
                     LGRYTCRKPVLVYWESEQRWTSVNGLEVMSMFGEHCHHIYLDDIKKKIQTELPENKTL
                     DFKRQLQ"
BASE COUNT          214 a           92 c          136 g          190 t
ORIGIN      
        1 tctggcatga tggtgtttta gtagaaacca gaatggaatg tcaccacacc gagtataatt
       61 ttatattgac ggcggagctt tatgttgatg aatttacagc cggaagggaa cgaaaaaggt
      121 tcgagtttac aggagtggaa catttatcgc aggttatgga tattgctgaa cttatagata
      181 accaaaatgc aggtaatatt aaagatgcga caattgttta taaggcaaag aagtataaaa
      241 tttcacttca tttgtttggt ggttttttaa gttttacatt taaaaccatg cgagttattg
      301 acaatgaatg tgcgtaatgt caaattcgac aacttgtctt tttatgaaga aaaaatccgc
      361 tggcgaatgc gtcggcaaat ctcgaatgca gaggcaatat tttacgagaa tcaggaagaa
      421 cacattcggc gctttttagg cagatatacc tgtcgcaaac cggttcttgt ttattgggaa
      481 agtgaacaaa gatggacatc tgtaaacggt ctggaagtga tgagtatgtt tggtgaacat
      541 tgtcatcaca tctaccttga tgatatcaag aaaaaaatac aaactgaatt acctgaaaat
      601 aaaactttag atttcaaacg tcaattgcaa tt
//
LOCUS       DAZKIF010000439          629 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZKIF010000439 DAZKIF010000000
VERSION     DAZKIF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.443"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..589)
                     /locus_tag="ACHOTW_005231"
     CDS             complement(<1..589)
                     /locus_tag="ACHOTW_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFP9847116.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSV"
BASE COUNT          173 a          156 c          142 g          158 t
ORIGIN      
        1 taactgagtg ataatattca agataaccac cggttaaatt taacagccga cgcgccatcg
       61 catcgccaga aaatgttcca ccagaaacag ccgtcccgac atggacataa cgtgaagctg
      121 ggccatattg ttgcgtgatc cttttcaggt tatcggcaat taaggtcgtc gcctcatccc
      181 atgagatacg ctcaaattta ccctcgcctc gtttacctac ccgcttcatc ggatatttca
      241 aacggtctgg atgataaaca aatttacgat agccgcgacc acgaacacag gcgcgcataa
      301 tcggcatttg tggatccaat tcgttatcgg gaagcgtggt aatttgcgtc accacgccgt
      361 cgcgcatatg cgcccgaata tcgcacttac caccgcagtc aaatgtgcta caggtgggca
      421 cgactttatc agctactgga gctgtaactt ctggcgacgc atctggggat tgcgcctttg
      481 cggtagaaga aataaaaggc aaagtgataa gcgcggaagt cgcttgtaaa aaactacgtc
      541 gtgatacttg cggaaatgta atgcgttcct tttcgtctgg cgtatccatc ttgcgttccc
      601 gaaaatgaaa aaataaatat tcacgtatt
//
LOCUS       DAZKIF010000440          625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZKIF010000440 DAZKIF010000000
VERSION     DAZKIF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.444"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>625
                     /locus_tag="ACHOTW_005232"
     CDS             <1..>625
                     /locus_tag="ACHOTW_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9847117.1"
                     /translation="DALDRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDES
                     DRLTHRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNP
                     ETGELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRD
                     RLHRETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNGD"
BASE COUNT          148 a          179 c          208 g           90 t
ORIGIN      
        1 gatgcgcttg accggctggt acagcagcgc ggctttgacg ggcggacgca acgttaccac
       61 tatgacctga ccggaaaact cacgcagagt gaagatgagg ggcttgtcac cctctggcac
      121 tacgacgaat cggaccgcct cactcaccgc acggtgaacg gcgaaccggc agagcagtgg
      181 cagtacgacg agcacggctg gctgacagaa atcagccacc tgagcgaagg ccatcaggtg
      241 gcggtgcatt acggttatga tgataagggc cgcctggccg gggagcgcca gacggtgcat
      301 aacccggaga cgggggaact gctgtggcag catgagacag agcacgcata caacgaacag
      361 ggtctggcaa accgcgtcac gccggacagc ctgccgcggg tggagtggct gacctacggc
      421 agcggttatc ttgcggggat gaagctgggc gggacgccgc tggtggagtt cacgcgcgac
      481 aggctgcacc gcgagacggt gcgcagcttc ggcaataacg catacgaact gaccagcaca
      541 tacactcccg caggccattt acagagccag cgcctgaaca gccaggtgta tgaccgtgac
      601 tacgactgga atgacaatgg cgacc
//
LOCUS       DAZKIF010000441          625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZKIF010000441 DAZKIF010000000
VERSION     DAZKIF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.445"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..267
                     /locus_tag="ACHOTW_005233"
     CDS             <1..267
                     /locus_tag="ACHOTW_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFP9847118.1"
                     /translation="FASSLEVFVREVFKDDVFKALKCYISSSIEPVFYEDHNYAFIRC
                     NAVLKQAVLTPIAHSVLSKICEANDKKGICHHLVNDGEVIWTVR"
     gene            267..>625
                     /locus_tag="ACHOTW_005234"
     CDS             267..>625
                     /locus_tag="ACHOTW_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFP9847119.1"
                     /translation="MVRIYNSSLEVACRMAKVLVAIYPSSLSLERLICFDFILVNLKD
                     FLPEEISLHPPIPRRDAQLALKREIVLESLALLQGYELASKIYTHRGFVYKASEKTYA
                     FTNSLHNEYVAQMEHNI"
BASE COUNT          196 a           95 c          117 g          217 t
ORIGIN      
        1 tttgcatctt cgcttgaggt ttttgtccgc gaagtattta aagatgatgt attcaaagca
       61 ttgaaatgtt acatttcatc ttcaattgaa cccgtctttt atgaagacca taattatgca
      121 tttattaggt gtaatgcagt cttgaagcag gctgttctga caccaattgc acattcagta
      181 ctatcaaaaa tatgtgaagc aaatgataaa aaaggaatat gccatcattt ggttaatgat
      241 ggtgaagtaa tttggacggt gagataatgg ttagaattta taattcaagt ttagaagtgg
      301 catgtcgaat ggcgaaagtg ctcgtcgcta tttatccttc ttcattaagc cttgaacggc
      361 ttatttgttt tgattttatt ttagtaaatc ttaaggattt tttacctgaa gagattagtc
      421 ttcatcctcc aataccccgt agagatgctc agttagccct aaaacgagag attgttttag
      481 aatcattggc tttgttgcaa ggctatgaac tagcctcaaa aatttataca catcgtggtt
      541 ttgtatataa agcttctgaa aaaacatatg catttacaaa ttctctacat aatgaatatg
      601 ttgcgcagat ggagcataat ataaa
//
LOCUS       DAZKIF010000442          621 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZKIF010000442 DAZKIF010000000
VERSION     DAZKIF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.446"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..409)
                     /locus_tag="ACHOTW_005235"
     CDS             complement(<1..409)
                     /locus_tag="ACHOTW_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFP9847120.1"
                     /translation="MNNTLLPSINIPCTLFETISLFDNYSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     FFGQYKTLIEDLIEHFRYGNGSNFHSQQLNLSFH"
     gene            545..>621
                     /locus_tag="ACHOTW_005236"
     CDS             545..>621
                     /locus_tag="ACHOTW_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HFP9847121.1"
                     /translation="MLIVNNKILNLPIRKLFPTPIASSYG"
BASE COUNT          213 a           94 c          103 g          211 t
ORIGIN      
        1 catgaaaaga taaatttaat tgttggctat gaaaattgct gccatttcca tatctaaaat
       61 gttcaatcag atcttcgata agtgttttat attgtccaaa aaaggaaaac atttttgcta
      121 gttctttcat ttctgcaaat aaaatatcag tgcattcacg ttgtgatatt tttgttccgg
      181 aacttgaggc actaaaagca acattaatag aacctgggcc agggaaatgg tatttaataa
      241 gtctgtaagg atcaactttt gctgagatat cgcttaggcc gagtgagaga aaatcttgtt
      301 ctaccatatc accatattgc atatcatcag cagaataatt atcaaataaa ctaatagttt
      361 cgaaaagtgt acagggaata ttaattgatg gaagtaatgt gttattcatt tacattttcc
      421 ttggttatat ttaagctcaa tatcaatttg agaacaaatg ctagggtaaa aaaatattta
      481 aaaaagaagt acacttcaca ttttttcatt acatctctga agagaacgtc gttaatgatt
      541 ggtcttgctc attgttaata acaaaatatt gaatttaccg attaggaaac tttttcctac
      601 gccgattgcc tccagctatg g
//
LOCUS       DAZKIF010000443          611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZKIF010000443 DAZKIF010000000
VERSION     DAZKIF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.447"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>611
                     /locus_tag="ACHOTW_005237"
     CDS             <1..>611
                     /locus_tag="ACHOTW_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFP9847122.1"
                     /translation="LDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKA
                     FEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCEVPDELIDFTFEWKGLKKLCVA
                     VSFRSIIAEQKKEPKMTVRYYISSADLTAGKFATAIRNHWHVENKLHWRLDVVMNEDD
                     CKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMD"
BASE COUNT          218 a          100 c          153 g          140 t
ORIGIN      
        1 tgctggatat taaaggaaaa atcatcacaa ctgatgcgat gggttgccag aaagatattg
       61 cagagaagat acaaaaacag ggaggtgatt atttattcgc ggtaaaagga aaccaggggc
      121 ggctaaataa agcctttgag gaaaaatttc cgctgaaaga attaaataat ccagagcatg
      181 acagttacgc aattagtgaa aagagtcacg gcagagaaga aatccgtctt catattgttt
      241 gcgaagtccc tgatgaactt attgatttca cgtttgaatg gaaagggctg aagaaattat
      301 gcgtggcagt ctcctttcgg tccataatag cagaacaaaa gaaagagcca aaaatgacgg
      361 tcagatatta tatcagttct gctgatttaa ccgcagggaa gttcgccaca gcaatccgaa
      421 accactggca cgtggagaat aagctgcact ggcgtctgga cgtggtaatg aatgaagacg
      481 actgcaaaat aagaagagga aatgcagcag aattattttc agggatacgg cacattgcta
      541 ttaatatttt gacgaatgat aaggtattca aggcagggtt aagacgtaag atgcgaaaag
      601 cagccatgga c
//
LOCUS       DAZKIF010000444          608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZKIF010000444 DAZKIF010000000
VERSION     DAZKIF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.448"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>608
                     /locus_tag="ACHOTW_005238"
     CDS             <1..>608
                     /locus_tag="ACHOTW_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847123.1"
                     /translation="YDYSRRYAINENIAKDAVTNNIALMSTFYDSDNTSGEVLNNDFT
                     EESFETALDVEHTIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYND
                     ICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLINLKTLLNDGCFIKY
                     SHFNDVALIKQNISNLYLSHTVVNESILKKCCFENGSLGDVK"
BASE COUNT          206 a           85 c          118 g          199 t
ORIGIN      
        1 tatgattata gcaggcgata tgcaattaat gaaaatattg caaaagacgc tgtaacaaac
       61 aatatcgcat tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat
      121 aatgatttta cagaagagtc atttgaaact gcgttagatg tagagcatac aatagtgctt
      181 ggatttgctg atgataattt tgaaacaaaa cctgttcatt atcaggattt attaacacga
      241 tttagtgcct ttcaagatac cgtgtttaat ctgtttcctg aaatgcactc ttcgcattac
      301 aacgatattt gtagcgtaag tgtggatatg acaaagggaa cgcaatgcat gattcacttg
      361 atggtgaatg aagaagtttt catgtctctg cctgttccag ttgccacgat ggtacgtgaa
      421 gatgcaagta atttaatcaa tcttaaaaca ctactcaatg atggttgctt tattaaatat
      481 agtcatttta atgacgtggc attaataaag caaaacataa gtaatctcta tttatcacac
      541 actgttgtga atgaaagcat attgaaaaaa tgttgttttg aaaacggcag tttaggtgat
      601 gttaaaat
//
LOCUS       DAZKIF010000445          601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZKIF010000445 DAZKIF010000000
VERSION     DAZKIF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.449"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..502)
                     /locus_tag="ACHOTW_005239"
                     /pseudo
     CDS             complement(1..502)
                     /locus_tag="ACHOTW_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
BASE COUNT          217 a          109 c           87 g          188 t
ORIGIN      
        1 ctggcattaa caatataaaa agcaatgaaa atgataacaa aaagaaaaaa aagcattaca
       61 tactttaaca gtttactgct aaagaaatgt tgttttgtag aaataatgct attttcaata
      121 atgcctggca atgaatactg ccaaacttca ttttttcttt tacagcaaat ttgagaccta
      181 aaacttctat ttgctgattc aatgttaacg ttctatcttc aggttcttgt gctgttaatt
      241 ctctgattgt tacaaccaga tcagggtcgg tagtgattat aatctcaaaa ctacctgtaa
      301 gctcgccata aggaacgact attgtatttt ctggtaactc agaaaaagca tcacttcttc
      361 ctaacgaaac atcattacca atgataacaa gtagacgccc attaagtatt tcatactcgc
      421 atccattcta tggtccgctc aacaaacgaa tggcataaga ttccaataag tcttgtgata
      481 agaatttatc attattttcc atgaatacca cccgctaatt ccataagctc tagtttagag
      541 tagtgtaaca agacggaaat ttcatgacca aattatgatg agacagaata aaaaaattat
      601 a
//
LOCUS       DAZKIF010000446          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZKIF010000446 DAZKIF010000000
VERSION     DAZKIF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.450"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            75..>593
                     /locus_tag="ACHOTW_005240"
     CDS             75..>593
                     /locus_tag="ACHOTW_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9847124.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTN"
BASE COUNT          181 a          108 c          137 g          167 t
ORIGIN      
        1 caattattgt tattattaag tctaatgtca tttaaaggca ttatcatatt tacgaagaga
       61 tcaggatgat aacgatgaaa aaaagtgtat tgacggcgtt tataactgtg gtatgtgcaa
      121 cgtccagcgt tatggctgct gatgataatg ctatcacgga tggctcagta acatttaatg
      181 gtaaagttat tgctccagct tgtaccctgg tagctgcgac gaaagattcc gtggtgactt
      241 tgccagatgt tagtgccacg aagttgcaaa ccaatggtca ggtttctggc gtgcaaactg
      301 atgtgccaat tgaattaaaa gattgtgata ctaccgtaac aaaaaatgca acgttcacct
      361 ttaatggcac tgcggatact actcagatta cagcgtttgc taaccaggcc tcatctgatg
      421 ctgctacaaa cgtggccctg caaatgtata tgaatgatgg tacaacggcc atcaagccag
      481 acacagaaac cgggaacatt ttgttgcaag atggagatca gacgttgact tttaaagttg
      541 attatatcgc tacggggaaa gcgacttctg gtaatgtgaa tgcggtaaca aat
//
LOCUS       DAZKIF010000447          589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZKIF010000447 DAZKIF010000000
VERSION     DAZKIF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.451"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..458)
                     /locus_tag="ACHOTW_005241"
     CDS             complement(<1..458)
                     /locus_tag="ACHOTW_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9847125.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLA"
     gene            complement(458..>589)
                     /locus_tag="ACHOTW_005242"
     CDS             complement(458..>589)
                     /locus_tag="ACHOTW_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9847126.1"
                     /translation="GWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSAEFKQVVN"
BASE COUNT          125 a          220 c          127 g          117 t
ORIGIN      
        1 gccagcacaa acgaggccgt catggccgtc agcgatgaca tctgctccac cacccaccgg
       61 tccgtcagct cctgctcagg gtctgcctca ggattgcctg ccacaaaatt caccgcatcc
      121 agaaaacggg catacacccg gcggcggacc accgtggccc ccaccaggct ctgcaggtcc
      181 tccgccatcc cggtgaccag accaaacaga ttcgacaccg tcagcgacgg tctggcactg
      241 ctgcccttcc cgttcatctc aaagccactg ccgtcaatcg ggtatgcctg atattgccgc
      301 ccctgccagg taaccgcctc ccctttttca ttcagctcat tgcagaaaaa ataccgctca
      361 ccgccctgcg ccgtcaggtc gatttcccag agtaccaccc gcggtgactg ctctgattta
      421 accgactcgt tcagactttc ttcgtgaata tcctgcatca gttcaccacc tgcttaaact
      481 ccgcgctgaa ctcaacgcgc aacatcccga cccgcgcaga ccacccggca caggtcacct
      541 ttatctgccg gtatgcatag ggtggcttcc acaaaaatgc cttccagcc
//
LOCUS       DAZKIF010000448          578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZKIF010000448 DAZKIF010000000
VERSION     DAZKIF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.452"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            141..533
                     /gene="ydeI"
                     /locus_tag="ACHOTW_005243"
     CDS             141..533
                     /gene="ydeI"
                     /locus_tag="ACHOTW_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP9847127.1"
                     /translation="MKFQAIVLASFLVIPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          173 a          111 c          134 g          160 t
ORIGIN      
        1 tgagttaaat tctctgaata tttgacagct agttggctga ttttaaatgc cttataacaa
       61 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
      121 aacgcaatta aggagttagt atgaaatttc aggccattgt attagcaagt tttcttgtca
      181 taccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
      241 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg actgttgatt
      301 tcgcaaaaac catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
      361 aaggagagga ccgttacgtt tttcgcgata agagtggtga aattaatgtt gttattcctg
      421 cgaccgtttt tgatggacga gaagttcagc cggaccagat gatcaacatt agcggcagtc
      481 tggataagaa aagcgcgccg gctgtcgttc gggtcactca tttgcagaaa taaaaataaa
      541 ttagcctgat ggcctgaact accgggccat ttgggtac
//
LOCUS       DAZKIF010000449          573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZKIF010000449 DAZKIF010000000
VERSION     DAZKIF010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.453"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>573
                     /locus_tag="ACHOTW_005244"
     CDS             <1..>573
                     /locus_tag="ACHOTW_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFP9847128.1"
                     /translation="IFEEHGLIKYVNLSFDIVASVGQVVGGVGALKFGKVVHSNRIKG
                     IGVTLVAHGANNFYESLSPLFFNEYDSGPIRELYRVIAKKMGGDINSADYAYSIVDFS
                     ITAYGGYSGMKIVPKYNRLIRPSLGNRPGTGRLFHYTSVDFKNKFSLKPTPLKIIQIS
                     STVKKFKVTFYDENTNLRIKHGLNNHMPHQL"
BASE COUNT          197 a           85 c          108 g          183 t
ORIGIN      
        1 attttcgaag aacatggttt gataaaatat gtgaacttaa gtttcgatat tgttgcatct
       61 gtaggacagg tcgtaggcgg ggttggcgcg ttgaaatttg gtaaggtagt gcattcaaac
      121 cgaataaaag gtataggcgt cacattagtt gctcatgggg cgaataattt ttatgaaagc
      181 ttatcacctt tattttttaa cgagtatgat tcaggtccaa tacgtgaact ctacagagta
      241 atagcaaaaa aaatgggagg tgatattaat tctgctgatt atgcatacag catcgtggac
      301 ttttcgatta ctgcatatgg tggatattca ggcatgaaaa tcgtacctaa gtataataga
      361 ttgattcgtc cttcattagg aaatagacct ggtacaggac gattatttca ttacacatca
      421 gttgatttta aaaataaatt ctcattaaaa cctacccctt taaaaatcat acaaattagt
      481 tctactgtaa aaaaattcaa ggttacattt tatgatgaaa atacaaattt gaggataaaa
      541 catggcctca ataatcatat gccacatcaa tta
//
LOCUS       DAZKIF010000450          572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZKIF010000450 DAZKIF010000000
VERSION     DAZKIF010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.454"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..198
                     /locus_tag="ACHOTW_005245"
     CDS             16..198
                     /locus_tag="ACHOTW_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9847129.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            171..362
                     /locus_tag="ACHOTW_005246"
     CDS             171..362
                     /locus_tag="ACHOTW_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9847130.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            373..>572
                     /locus_tag="ACHOTW_005247"
     CDS             373..>572
                     /locus_tag="ACHOTW_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847131.1"
                     /translation="MHFSGSRLHILCAYACRHGTCSMTPQQENALRSIARQANSEIKK
                     ARQQFPDKNVDDICRSVLKKHR"
BASE COUNT          174 a          133 c          139 g          126 t
ORIGIN      
        1 tggggagcaa tggtaatgaa gcatcctcac gataatatcc gggtaggcac gatcactttc
       61 gtctactccg ttacaaagcg aggctgggta tttcccggcc tttctgttat ccgaaatcca
      121 ctgaaagcac agcggctggc tgaagagata aataataaac gaggggctgt atgcacaaag
      181 catctcctgt tgaattaaga acgagtatcg ggatggcaca tagcctcgct caaattggag
      241 tcaggtttgt gccaatacca gtagaaacag acgaagaatt tcatacgtta gccacatccc
      301 tttcacaaaa gctggaaatg atggcggcga aagcagaagc aaacgagaga gacccggcat
      361 gacaacaaca gaatgcattt ttctggcagc aggcttcata ttctgtgtgc ttatgcttgc
      421 cgacatggga cttgttcaat gacacctcag caggaaaacg cccttcgcag cattgcccgt
      481 caggctaatt ctgaaatcaa aaaagccaga cagcagtttc cggataaaaa cgtcgatgac
      541 atttgccgta gcgtactgaa gaagcaccgc ga
//
LOCUS       DAZKIF010000451          557 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZKIF010000451 DAZKIF010000000
VERSION     DAZKIF010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.455"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..535
                     /locus_tag="ACHOTW_005248"
     CDS             2..535
                     /locus_tag="ACHOTW_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9847132.1"
                     /translation="MFTIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSPGSLPFTT
                     AATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWI
                     YLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAA
                     KQHYKNAERLLAGLPAH"
BASE COUNT          172 a          128 c          120 g          137 t
ORIGIN      
        1 tatattcaca atttttatag ttgcagcaat tacctgtatt agccaaccca agaaaacgac
       61 gttgcgtgat aaagccatgg tgaattatgc ctttgattat ttaagctcac cgggcagtct
      121 tccattcacc acggcagcca cggagctttc cgcgattcat ggtcactcaa cgtcgcaata
      181 tcgccttgga gaattttatc ttcatggtag cgacggtaaa ccactggatt atacacaggc
      241 gagatactgg tatgagcaat cagcggaaca ggaaaatcca cgcgcgcaaa gtaaactggg
      301 gtggatctac ctcaaaggtc tgggggtcaa acccgacacc cgtaaagcaa ttctctggta
      361 taaggaagca gctgaacaag ggtatgctca tgctcaatat actttaggtt tgatctacag
      421 aaatggctca ggtattaatg ttaaccatta tgaatctcaa aaatggttaa aactggccgc
      481 caaacaacat tacaaaaatg cggaaagatt acttgccggg cttcccgcac attaacgtcg
      541 actgtcaccc gaacaga
//
LOCUS       DAZKIF010000452          556 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZKIF010000452 DAZKIF010000000
VERSION     DAZKIF010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.456"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            26..340
                     /locus_tag="ACHOTW_005249"
     CDS             26..340
                     /locus_tag="ACHOTW_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9847133.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
     gene            complement(422..556)
                     /locus_tag="ACHOTW_005250"
     CDS             complement(422..556)
                     /locus_tag="ACHOTW_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HFP9847134.1"
                     /translation="MKGSGNFSAWVIEACRRRLINEKYSQFVPNKDKHDQRTCSDRFT
                     "
BASE COUNT          173 a          101 c          143 g          139 t
ORIGIN      
        1 gtctgtagat aatcggaggt cacttatgct acatgatcac gtggcagaat gtctggagaa
       61 aaaaggactg taccggagag cagctgaacg atgggcaaaa gtgatggtac agctaagtga
      121 tgaccagaaa agaaaagtgg cggcacagaa acgagcagag tgtttgcgta aggcgcgccg
      181 gactccggtt tcaccggtga acctgaccga aataaaacaa gcggtcaaca gactacattc
      241 tgagttggga atgggatttg aagagcggcg ggtattccga cgatataaag ggacaggaga
      301 acagaatacg tccggaaacg cgcggtcaaa aaaatgctaa aaaatatctg agagcgttat
      361 tgcctgttac cataagaaaa agcgacttta gtggtcgctt tttgtgtcat atataagtcg
      421 tttaagtaaa cctgtctgaa caggttctct ggtcgtgttt gtctttgttg ggtacaaatt
      481 gagaatattt ttcattaatt aatcttcttc tgcaggcttc aataacccac gctgaaaaat
      541 ttcctgaacc tttcag
//
LOCUS       DAZKIF010000453          551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZKIF010000453 DAZKIF010000000
VERSION     DAZKIF010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.457"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>551
                     /locus_tag="ACHOTW_005251"
     CDS             <1..>551
                     /locus_tag="ACHOTW_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFP9847135.1"
                     /translation="DDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQLDKLRRM
                     NFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPR
                     DEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQA
                     PAPSRPIERGIAGPGLLARVLTS"
BASE COUNT          120 a          169 c          167 g           95 t
ORIGIN      
        1 tgacgacatc ttcctgctga aacagcgcct ggccgaacag gaagcgctga tccacgccct
       61 gcaggaaaag ctgagcaacc gggagcgcga aatagaccat ctgcaggcgc agctggataa
      121 actccgccgg atgaacttcg gcagtcgttc cgaaaaagtc tcccgccgta tcgcacaaat
      181 ggaagccgat ctgaaccggc ttcagaaaga gagcgatacg ctgactggta gggtgtatga
      241 cccggcagta cagcgtccgt tgcgtcagac ccgcacccgt aagccgttcc ctgaatcact
      301 accccgtgac gaaaagcgac tgttgcctgc ggcgccgtgc tgcccgaact gcggcggttc
      361 actgagctat ctgggcgagg ataccgccga acagctggag ttgatgcgta gcgccttccg
      421 ggttatccgg acggtacggg aaaaacatgc ctgtactcag tgcgatgcca tcgtgcaggc
      481 acctgcacct tcgcggccca tcgagcgggg tatcgccgga ccggggctgc tggcccgcgt
      541 gctgacctcg a
//
LOCUS       DAZKIF010000454          544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZKIF010000454 DAZKIF010000000
VERSION     DAZKIF010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.458"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>544
                     /gene="rfbE"
                     /locus_tag="ACHOTW_005252"
                     /gene_synonym="perA"
     CDS             <1..>544
                     /gene="rfbE"
                     /locus_tag="ACHOTW_005252"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFP9847136.1"
                     /translation="DISTFSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHR
                     QYWHDVIGYNYRMTNICAAIGLAQLEQADDFISRKREIADIYKKNINSLVQVHKESKD
                     VFHTYWMVSILTRTAEEREELRNHLADKLIETRPVFYPVHTMPMYSEKYQKHPIAEDL
                     GWRGINLPSFPSLSNEQVIYI"
BASE COUNT          185 a           93 c          106 g          160 t
ORIGIN      
        1 agatatttct acttttagct tttttggaaa taaaactatt actacaggtg aaggtggaat
       61 ggttgtcacg aatgacaaaa cactttatga ccgttgttta cattttaaag gccaaggatt
      121 agctgtacat aggcaatatt ggcatgacgt tataggctac aattatagga tgacaaatat
      181 ctgcgctgct ataggattag cccagttaga acaagctgat gattttatat cacgaaaacg
      241 tgaaattgct gatatttata aaaaaaatat caacagtctt gtacaagtcc acaaggaaag
      301 taaagatgtt tttcacactt attggatggt ctcaattcta actaggaccg cagaggaaag
      361 agaggaatta aggaatcacc ttgcagataa actcatcgaa acaaggccag ttttttaccc
      421 tgtccacacg atgccaatgt actcggaaaa atatcaaaag caccctatag ctgaggatct
      481 tggttggcgt ggaattaatt tacctagttt ccccagccta tcgaatgagc aagttattta
      541 tatt
//
LOCUS       DAZKIF010000455          543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZKIF010000455 DAZKIF010000000
VERSION     DAZKIF010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.459"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            86..>543
                     /gene="tccP"
                     /locus_tag="ACHOTW_005253"
                     /gene_synonym="espF(U)"
     CDS             86..>543
                     /gene="tccP"
                     /locus_tag="ACHOTW_005253"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HFP9847137.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLVQHLAEHGIQPARNMAEH"
BASE COUNT          165 a          148 c           91 g          139 t
ORIGIN      
        1 tatacatcgc cgcatttatt tgccactata aaaaggctga ttacatatct atcggagaac
       61 taaaattact tataaggtaa tctgcatgat taacaatgtt tcttcacttt ttccaaccgt
      121 caaccgcaat attacagctg tatataaaaa aagcagcttc tctgtatcac cacagaaaat
      181 cacattaaat cctgtaaaaa tcagctcacc tttttcacca agcagtagct ccatcagcgc
      241 aacaactctc tttcgagccc caaacgccca ttcggcatca tttcatcgac agtctactgc
      301 tgaaagttcg ttacatcaac aacttcctaa tgtgaggcag cgcctgatac aacatcttgc
      361 agagcatggc attaaacctg cccggagtat ggctgaacat attcctccgg cacctaactg
      421 gcctgcgcca ccaccgccag tacaaaatga acaatcaaga cctctgcctg atgtggctca
      481 gcgtctggtg cagcatcttg cagagcatgg cattcaacca gcccggaata tggctgaaca
      541 tat
//
LOCUS       DAZKIF010000456          535 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZKIF010000456 DAZKIF010000000
VERSION     DAZKIF010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.460"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(338..>535)
                     /locus_tag="ACHOTW_005254"
     CDS             complement(338..>535)
                     /locus_tag="ACHOTW_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9847138.1"
                     /translation="TTRNIRFPNQMIEQINIALDQKGSENFSAWVIESCRRELAADIK
                     YARQLTIKKNDTQYALRWLFI"
BASE COUNT          145 a          105 c          125 g          160 t
ORIGIN      
        1 ccgtattcct gcattaacag agaccgcagc ccgacaggga gactcctctg cgagagtgtg
       61 cggggataat caaaaacgat acacaccggg gtttaccgcg taaacggagc gcggcgttct
      121 cccctcatgg tcgcccgtcc ggtgcgatgg tggaagaaac tggaatctgt tcaataaaaa
      181 aactgccgtg ttggagtcac agcagtaatg tactgattgg gtagaagatt attattgtta
      241 tgctttattt ttattctata tggctgatta tttcaattcg gaattaatac agctaatgtc
      301 tgtgagtttt tataaattca gcaatataaa gaaatagtta tatgaacagc catcgcagag
      361 catactgtgt atcattcttt tttatagtca actgacgggc atattttatg tctgctgcca
      421 gctcccggcg gcaagattca atgacccacg cagaaaaatt ttctgaacct ttctggtcaa
      481 gagcgatgtt aatttgttca atcatctggt ttggaaatcg gatgttgcgg gttgt
//
LOCUS       DAZKIF010000457          530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZKIF010000457 DAZKIF010000000
VERSION     DAZKIF010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.461"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>530
                     /locus_tag="ACHOTW_005255"
     rRNA            <1..>530
                     /locus_tag="ACHOTW_005255"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          143 a          120 c          167 g          100 t
ORIGIN      
        1 agacccgaaa cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga
       61 ggaccgaacc gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc
      121 aatcaaaccg ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt
      181 catctccggg ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg
      241 caaactgcga ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt
      301 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga
      361 aacgatgtgg gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag
      421 aaagcgtaat agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca
      481 tgcaccgaag ctgcggcagc gacactgtgt gttgttgggt aggggagcgt
//
LOCUS       DAZKIF010000458          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZKIF010000458 DAZKIF010000000
VERSION     DAZKIF010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.462"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..470
                     /locus_tag="ACHOTW_005256"
     CDS             3..470
                     /locus_tag="ACHOTW_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFP9847139.1"
                     /translation="MLCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVVDHLPWTDS
                     DKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKS
                     NDNCITKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIEQVFNPHNFKKDNF"
BASE COUNT          182 a           92 c           83 g          169 t
ORIGIN      
        1 ttatattatg tttatttctt ctatgctgca tctataattt atggacattg cgtcctgtac
       61 agattttgta tacatactct gatgctggca attcagtctt tctggtcgtt gatcatctgc
      121 catggactga cagcgataaa ataaattggt atctgaagca tcagaatgaa attaaaaatc
      181 aacatcccct accagaaggt agttggcata cctggtatgt cattgatatc ggtaatggct
      241 tcactgacta taaaaaatat atcgaagggc cgtatgaaga tctctactgt ttcccgacaa
      301 ttaagagcaa tgataattgc atcacgaaaa attacttaat ggttattaat gaataccctt
      361 atcgtaacac tcatattggt attaacgatt ttactgagta tcaattaaca caagaaaata
      421 aaatagagca agttttcaat ccgcataact ttaaaaaaga caatttttag aaataagccg
      481 gataaaaaaa tagctttatc cggcactcat attacaacca gttctt
//
LOCUS       DAZKIF010000459          525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZKIF010000459 DAZKIF010000000
VERSION     DAZKIF010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.463"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..525
                     /locus_tag="ACHOTW_005257"
     CDS             <1..525
                     /locus_tag="ACHOTW_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9847140.1"
                     /translation="LGELMVSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERT
                     LNHGSLTSREVTVLREIEMLENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRT
                     CPVTLCEPEDGVFMRNSMNSNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDN
                     CAFDSDRGNFVLKN"
BASE COUNT          169 a           71 c          121 g          164 t
ORIGIN      
        1 ctgggtgagt tgatggtgtc aatacgacct atgcaggtaa atggatattt tatgggaagt
       61 cttaaccagg atggtttatc gaatgataat atccagattg gccttcaata tatagaacat
      121 attgaacgta cacttaatca tggtagtttg acaagccgtg aagttacagt actgcgtgaa
      181 attgagatgc tcgaaaatat ggatttgctt tctaactacc agttagagga gttgttagat
      241 aaaattgaag tatgtgcatt taatgtggag catgcacaat tgcaagtgcc agagagctta
      301 cgaacatgcc ctgttacatt atgtgaacca gaagatgggg tatttatgag gaattcaatg
      361 aattcaaatg tttgtatgtt gtatgataaa atggcattaa tacatcttgt taaaacaagg
      421 gcggctcatc ctttgagcag ggaatcaatc gcagtttcaa tgattgtagg aagagataat
      481 tgtgcttttg actctgacag aggtaacttc gttttaaaaa attaa
//
LOCUS       DAZKIF010000460          521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZKIF010000460 DAZKIF010000000
VERSION     DAZKIF010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.464"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..95
                     /locus_tag="ACHOTW_005258"
     CDS             <1..95
                     /locus_tag="ACHOTW_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847141.1"
                     /translation="RSSLKENTETVEMNSKVWGRLKKIITFFKK"
     gene            complement(171..473)
                     /gene="bamE"
                     /locus_tag="ACHOTW_005259"
     CDS             complement(171..473)
                     /gene="bamE"
                     /locus_tag="ACHOTW_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP9847142.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVSTYIFNAGTSNVKTGIF"
BASE COUNT          145 a          100 c          110 g          166 t
ORIGIN      
        1 taagatcctc tttaaaggaa aatactgaga ctgtagaaat gaacagtaaa gtttggggga
       61 ggctgaaaaa gataatcact ttctttaaaa agtgatttat gtttctgtaa aaagcccgta
      121 ggtatcaact gcgggctaaa ttttcccttg atgctggttt tgtcttttat ctaaaaaatg
      181 ccagtcttca cgttgcttgt tccagcatta aagatgtatg tgctgacttt ttcgcctttg
      241 aaagaaactg tcagagattt agtttgtgag tctgcgccac ctgcaagcag tcccacaacg
      301 gggatgaaag aggttgcttt ggactggctg ttatacatag tgtatgacca ttgttcttca
      361 ccatcgatca aagaacggct gtcaggttca ccgaacgatg ctaacacgtc ctgttttgta
      421 gttttgcctt taacaatttt ggttttcaca ctttcctgcg tctcattttt caatgactgg
      481 ttgcctactg aagaacaacc tgccaaggtc agaaaaacga a
//
LOCUS       DAZKIF010000461          520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZKIF010000461 DAZKIF010000000
VERSION     DAZKIF010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.465"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..204
                     /locus_tag="ACHOTW_005260"
     CDS             <1..204
                     /locus_tag="ACHOTW_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9847143.1"
                     /translation="FRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDIT
                     KGATYPVLIDSIAVEVNNTDTAA"
     gene            342..>520
                     /locus_tag="ACHOTW_005261"
     CDS             342..>520
                     /locus_tag="ACHOTW_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9847144.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
BASE COUNT          146 a          115 c          139 g          120 t
ORIGIN      
        1 ttccgctttg ccgggaataa cagccttcag gtgacgccgg ttattgatgg tcaggatggc
       61 acaccgttca cgctgacgca gtcaccggtc agtgcatttg cggcggataa actgcatgtg
      121 acagacatta ccaaaggtgc gacttacccg gtgctgattg acagcattgc ggtggaagtg
      181 aacaacacag acactgcggc atgataaaaa aaaccgccag cgacaggaat ggacgctggc
      241 ggtggtaata cctatggaga aaaaataaag gaacgatact ttcgtgctct ggttttttaa
      301 atgaaaacag ttcttattgt caacaataac ggaaagaaat tatgacattt ctgaaccagt
      361 taatgctgta cttctgtacg gtggtctgtg tgctgtatct cctttcgggt gggtacaggg
      421 ccatgcgtga cttctggcgc agacagattg acaaaagggc cgctgagaaa atcagcgcca
      481 gtcagtcagc cggaagcaaa cccgaagagc cgctcattta
//
LOCUS       DAZKIF010000462          519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZKIF010000462 DAZKIF010000000
VERSION     DAZKIF010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.466"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            4..381
                     /locus_tag="ACHOTW_005262"
     CDS             4..381
                     /locus_tag="ACHOTW_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HFP9847145.1"
                     /translation="MNLDEEFYELQKLFAQKDLLAEKRRSSGGGFMEILLVKRQNMKI
                     KIYQEKGHQLPHIHIDYGKQAHAASYAIQSGDRIEGDLPKKYDSDVSSWLGRNRDKIL
                     EIWNALQAGAPYEPLIAELTGGV"
BASE COUNT          183 a           88 c          113 g          135 t
ORIGIN      
        1 tatatgaatt tagatgaaga attttacgaa ttacaaaaac tctttgctca gaaggatcta
       61 ctggctgaga aaagacgctc tagtggcggc ggattcatgg aaattctctt ggtgaagaga
      121 cagaacatga agataaaaat atatcaagaa aaaggacatc aacttcccca tattcatatt
      181 gactatggaa aacaagcgca tgctgccagt tatgccatac aatcaggaga ccgcatagag
      241 ggagatttac ctaagaaata tgatagtgat gtttcgagtt ggttggggag aaatcgtgat
      301 aaaattttag aaatctggaa tgcactacaa gctggcgcac cttatgaacc actaatcgca
      361 gaattgactg gcggagtgtg actaactaaa taattttccc ctaaaaaaat tcgatgtagg
      421 aaaatatata taagtaagaa tcatgagtgt ccgctcctcg ctcaaggcgg actgttagac
      481 ttgattgtat gctgtcagag aaaaccgtca gatcaattc
//
LOCUS       DAZKIF010000463          518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZKIF010000463 DAZKIF010000000
VERSION     DAZKIF010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.467"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>518
                     /locus_tag="ACHOTW_005263"
     CDS             <1..>518
                     /locus_tag="ACHOTW_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFP9847146.1"
                     /translation="SSARWMSKSEKNNKQKEAIEKFKKEVSHMNGGQQFIWGFDKVIQ
                     EGLSGLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVPGYNALSGVSS
                     ILHSAIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEALKHIEKNINDNI
                     IYHPSNNANFYS"
BASE COUNT          169 a           70 c          113 g          166 t
ORIGIN      
        1 tatcatctgc tcgctggatg tcaaaatcag aaaagaataa taaacaaaaa gaggcaatag
       61 aaaagttcaa aaaagaagta tcccatatga atggcgggca gcagtttatt tgggggtttg
      121 ataaggttat tcaagaaggc ttaagtggat tgattgagtt aagtatcgat attaatgata
      181 gtacaaatca tcgtgataag tcttctcttt ctcctgatgg gagagctgtg ttacattttt
      241 taggtacaat ttggaatatg gcgatgggag ctgtacctgg ttataatgca ttgtctggtg
      301 taagtagcat tttacatagt gctatagtaa aagaatctag caatatctgt gattatattc
      361 agggggctgt acgtattgga atggactttg ttccaggcac tcgctctgac ttacatagcc
      421 gttcgctgca gataaaatat gaggctttga agcatataga aaaaaacatt aatgataata
      481 ttatttatca tccgagtaat aatgctaatt tctattct
//
LOCUS       DAZKIF010000464          516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZKIF010000464 DAZKIF010000000
VERSION     DAZKIF010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.468"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..514
                     /locus_tag="ACHOTW_005264"
     CDS             <1..514
                     /locus_tag="ACHOTW_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFP9847147.1"
                     /translation="LLKEILRYITVQRISEVIQRLIQERISVRNMRLVMEALALWSPR
                     EKDIITLVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLD
                     ASEAEMILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTE
                     NVPVNILKTI"
BASE COUNT          157 a           90 c          112 g          157 t
ORIGIN      
        1 tctattaaaa gaaatattaa gatatataac cgtgcagcgc atttcagagg taattcagcg
       61 tctgatccag gagcgaattt cagtgcgcaa tatgcggtta gttatggagg ccttagcatt
      121 atggagccca cgagagaaag atataatcac actcgtcgag catgtacgag gtgcattagg
      181 tcgctacatt tgccataagt tttcatattc tggagaaatt aaagcaattg tgatttcgcc
      241 tgaaatagag gacagaatca gagatggtgt caggcctacc gctggcggta cttttcttaa
      301 tctcgatgca tccgaagctg agatgattct ggataatttt aaactggcac tttctggaat
      361 caatattcct attaaagaca ttattttact gggatctgtg gatattcgtc gttttatcaa
      421 aaaacttatt gaatctagtt atcgagacct cgaagtactt tcgtacgggg aactaacgga
      481 aaatgttccc gttaatattc tgaaaactat ttagga
//
LOCUS       DAZKIF010000465          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZKIF010000465 DAZKIF010000000
VERSION     DAZKIF010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.469"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..125
                     /locus_tag="ACHOTW_005265"
                     /pseudo
     CDS             1..125
                     /locus_tag="ACHOTW_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(320..>514)
                     /locus_tag="ACHOTW_005266"
     CDS             complement(320..>514)
                     /locus_tag="ACHOTW_005266"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9847148.1"
                     /translation="SGSSSGELPVTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVL
                     VCGIMVLLWPVIKRNSLHNA"
BASE COUNT          161 a          137 c          114 g          102 t
ORIGIN      
        1 cgacatcgaa tttctcccgt gtagaggaac agagttaaaa agccggaagc ggaatcaaat
       61 cacaggatga ccatctgcca gtggctggtc gtaaaaaaag gccgcgccat gcgcagccga
      121 aaataaaggg ataacgatga tagtttgaga aaaacagaaa taacactttt gtggcaaagc
      181 atggtgccgg gtgcctcccg gtgaattcag tatcagcacc tgaatccgcg attaccccat
      241 attccttctt gctgattgcc ccaccgcaca gggggattca ccatgcagaa gtgtttttaa
      301 taaacagcaa acaaaaaaat caagcattat gcaggctgtt tctttttatc accggccaca
      361 gcaataccat aatgccgcag accagcaccc catccgccag caccgacatg attctgctgg
      421 tgaaatccac catcaccacc agaaacagca ggagtgcagc cacagtcagg cgcagtttta
      481 ccgtcacagg taattctcca gacgaagacc caga
//
LOCUS       DAZKIF010000466          510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZKIF010000466 DAZKIF010000000
VERSION     DAZKIF010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.470"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..232
                     /locus_tag="ACHOTW_005267"
     CDS             <1..232
                     /locus_tag="ACHOTW_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847149.1"
                     /translation="LVIGEKIHFRTLSNRSLYFNLTHPPLIATIKDDVFCTLHDYYNH
                     NNETKSTIDNYISKIKDNHNTPWLLNNENVQE"
BASE COUNT          196 a          107 c           65 g          142 t
ORIGIN      
        1 tctggtcatt ggcgaaaaaa ttcatttcag gacactaagc aatcgttctt tatattttaa
       61 cctcacccac cccccattaa tcgccacaat aaaagatgat gtgttttgca ctcttcatga
      121 ttactataac cacaataatg aaacgaaatc cactattgac aattacatat caaaaataaa
      181 ggataatcat aataccccat ggttattaaa caacgaaaat gttcaggaat aaaaaaaaag
      241 agacaattcc cgagataaaa caaaagagca taaacatatg aaccggaaat tctatttcaa
      301 aaatatcctg tggggaatgt gccccattat aatatccata ttgcatctga tatatgacac
      361 accaacagaa gcatcgataa aagttgactt atttctgata ctcagtggcc tggcccttcc
      421 ttgttcaata tacctgacaa aaacaatcac actaaaatac acatccatat cattctggaa
      481 aaaacctctt ttctggtctt atggtatata
//
LOCUS       DAZKIF010000467          510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZKIF010000467 DAZKIF010000000
VERSION     DAZKIF010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.471"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(331..>510)
                     /locus_tag="ACHOTW_005268"
     CDS             complement(331..>510)
                     /locus_tag="ACHOTW_005268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847150.1"
                     /translation="YSKNTQAFRTLYQPHLDCFPFPDTAARGVIKVRNFQQYGVLLFT
                     AVMHQPVLAIVVITV"
BASE COUNT          151 a          106 c          110 g          143 t
ORIGIN      
        1 tttaccaagg tgtgtttcgt aacaattttg tgtacaatat gactctacat atcgacgaaa
       61 aaaccacttg atttgacagg aatacattca aaaaatttct tttttcattt caggagcgat
      121 tttcatctgt tacttgaagt atctgtgaga ttaagtgact ccccctaacg tcgtactaaa
      181 tagtaattac tggccatacc gggagcagta ttcggcgctc cccacatgca gcagaataac
      241 cgcatcagcg aagatgtatt tttactatca caatgtgtac ggacggttta tggaaaagaa
      301 tgaacattag atacgcgatg cggcggcagg ttacacggtt atcactacga tagccaatac
      361 tggctggtgc attaccgccg tgaacaatag gacgccgtac tgctggaaat ttcttacctt
      421 tataactccc cgcgccgccg tatcgggaaa cgggaagcaa tcaagatgag gctgatataa
      481 tgtgcggaat gcctgagtat ttttagaata
//
LOCUS       DAZKIF010000468          504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZKIF010000468 DAZKIF010000000
VERSION     DAZKIF010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.472"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..302
                     /locus_tag="ACHOTW_005269"
     CDS             <1..302
                     /locus_tag="ACHOTW_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9847151.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>504
                     /locus_tag="ACHOTW_005270"
     CDS             299..>504
                     /locus_tag="ACHOTW_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9847152.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALW"
BASE COUNT          114 a          117 c          169 g          104 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcagggac
      421 cagcattggc gcattcaatg gtgccattat cgcggcctct cccgatctgt cagaagctgc
      481 cgtacgcctg gaggcgctct ggga
//
LOCUS       DAZKIF010000469          498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZKIF010000469 DAZKIF010000000
VERSION     DAZKIF010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.473"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            103..498
                     /locus_tag="ACHOTW_005271"
                     /pseudo
     CDS             103..498
                     /locus_tag="ACHOTW_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          146 a          122 c          118 g          112 t
ORIGIN      
        1 aaaacagagg ctttttcctc tgctgtaaat gttcgtctca ttcaaaaaac ctccgcaacc
       61 ccatgttttc acataactgt tgcgttgacc aattgaatct acaacagcgt attttgcaca
      121 ggagtcgctg aaaaatacgc gttaatcgaa caatggcgac aacaatttcc cattgaagcg
      181 atgtgtcagg tatttggtgt atccaggagc ggttattaca actgggtaca gcatgaaccc
      241 tcagacagaa aacaaagtga tgagcggcta aaactggaga ttaaggtggc acatatccgc
      301 actcgcgaaa catatggaac ccggcggctc cagacggagc tggcagagaa tggcatcatc
      361 gttggtcgtg accgactggc acgtcttcgt aaggagctaa ggctacgctg taagcagaaa
      421 cgcaagttca gggcgactac gaactcgaac cacaatctgc cagttgcgcc aaatctgctg
      481 aaccagacgt tcgctcct
//
LOCUS       DAZKIF010000470          496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZKIF010000470 DAZKIF010000000
VERSION     DAZKIF010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.474"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            255..470
                     /locus_tag="ACHOTW_005272"
     CDS             255..470
                     /locus_tag="ACHOTW_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9847153.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          118 a           99 c          148 g          131 t
ORIGIN      
        1 ctggctaaga aggatagttg ggtttcacat gatacttata tctggcagta cattttctga
       61 cagacagtga tgggtgttgt caagatattg tgtcatttat aacctgaatc agggggggga
      121 gccggaatgt tatctggcat ttttagcaga gcctgaatgc cataatcacg gctcccggcg
      181 ttggccgtca gtgggcgaca ctggcggctt tttgttttcc tttactttca ttttctgtcg
      241 gcggtgacgg agacatacat cagatggaaa aaatcacaac aggtgtgtca tacaccacgt
      301 cagcggtggg gacgggatac tggttactgc agctgctgga caaagtctct ccgtcccagt
      361 gggtggcaat cggtgtgctg gggagtctgc tgtttggcct gctgacgtat ctgactaacc
      421 tgtatttcaa aatcagagag gaccgtcgta aggcggcgcg gggggagtaa agcgatgaag
      481 aaaaaatacg aactgg
//
LOCUS       DAZKIF010000471          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZKIF010000471 DAZKIF010000000
VERSION     DAZKIF010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.475"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            113..>483
                     /locus_tag="ACHOTW_005273"
     CDS             113..>483
                     /locus_tag="ACHOTW_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847154.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVL
                     SEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVLSNDNNLTLHCHVRGNEKLFVDV
                     YAKFIEPLVIKNTGMPQVYLK"
BASE COUNT          173 a           64 c           95 g          151 t
ORIGIN      
        1 atgatatatt acattgcaat ctggagaaaa agaaaggtct ccattattct tgatattgct
       61 tatggataga acaaattgaa aggagtgagg ttagtatgaa actgagtgag ttatgattat
      121 gaaggatggc atctatagca ttatatttat tagcaatgaa gactcctgtg gggaaggtat
      181 actgattaaa aatggaaata tgatcactgg cggcgatatt gcttctgtgt atcagggggt
      241 cctctctgaa gatgaggaca tcatacttca tgtccatcga tataattacg aaattccctc
      301 ggtgctaaac attgaacaag attatcaatt agttatccct aaaaaagtac tgagtaatga
      361 taataatctc acattacatt gccatgtaag aggaaatgaa aaattgtttg ttgatgttta
      421 tgccaaattt atagaaccat tagttattaa aaacacagga atgccacaag tttatttaaa
      481 ata
//
LOCUS       DAZKIF010000472          478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZKIF010000472 DAZKIF010000000
VERSION     DAZKIF010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.476"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>478
                     /locus_tag="ACHOTW_005274"
     CDS             <1..>478
                     /locus_tag="ACHOTW_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847155.1"
                     /translation="SSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQTSIE
                     MVTLLNTLQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSDNTCE
                     LTINEPLKNQEISTGNININCPLKDIYNEFRRLNVIFSCGTGDIVDLSSLDLRNVDL"
BASE COUNT          167 a           84 c           79 g          148 t
ORIGIN      
        1 ttcatcaatt ggtaatgata ttagtagtga tttagtccgt cgaaaaatga atgacctacc
       61 tgaaagccca acaggaaata acctcgaagc actagcacca ggtattgaaa aactaaaaca
      121 gacctctatt gaaatggtca ctttacttaa cacgttacaa cctagtggaa aatgcattat
      181 cactggtgat tttcaaaaag aattagcgta cttacaaaat gtaattcttt ataatgactc
      241 gtctcttcgt ctggattttt taggttataa cgcccaaatt attcaacgat cggacaatac
      301 ttgtgaactt accattaatg aaccgttaaa aaaccaggaa atatccacag gtaatatcaa
      361 tattaattgc ccattaaaag atatttacaa tgaattcagg aggttaaacg taatttttag
      421 ttgtgggact ggagatatcg ttgatctatc ctctctggac ttacgtaatg tcgattta
//
LOCUS       DAZKIF010000473          471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZKIF010000473 DAZKIF010000000
VERSION     DAZKIF010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.477"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>471)
                     /locus_tag="ACHOTW_005275"
     CDS             complement(<1..>471)
                     /locus_tag="ACHOTW_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9847156.1"
                     /translation="IYIINDEVRFNSSSNKLTSLLTSEEVILTYPAGKCLKLLLDNAY
                     SVVSHNQFFEKVWSDSSADVATNTLYQNISMIRRSFREISSATIFNDVIRTVPRKGFK
                     INEEISITVLDNNHNENESVESEENGTHTENINKNHIAKGIFTTKNTLLIVCFFA"
BASE COUNT          136 a           80 c           82 g          173 t
ORIGIN      
        1 cgcaaaaaaa caaactataa gtaatgtatt ctttgtggta aatatacctt ttgcaatgtg
       61 atttttattt atattttcag tgtgggtacc attctcttca ctctcaacac tttcattctc
      121 attgtgatta ttgtcgagaa cagttatgct gatttcttca ttgattttga agcctttacg
      181 gggcaccgtt ctgataacat cattaaatat cgtcgctgag ctgatttcac gaaaagaacg
      241 tcttatcatc gatatatttt gatataaagt gttggttgcg acatcggcac tgctatcgct
      301 ccagactttt tcaaagaatt gattgtgtga cacgacacta taagcgttat ctaaaagtaa
      361 ctttaagcat tttcccgcag gataagtgag tatcacttcc tctgatgtaa ggagtgatgt
      421 taacttatta gatgatgagt tgaatcttac ttcatcattt ataatgtaga t
//
LOCUS       DAZKIF010000474          469 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZKIF010000474 DAZKIF010000000
VERSION     DAZKIF010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.478"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>469
                     /locus_tag="ACHOTW_005276"
     CDS             <1..>469
                     /locus_tag="ACHOTW_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9847157.1"
                     /translation="RLESGIRISIGSGQYSVHYVQLLDGFSVEPVRGGLLDRLLGREH
                     RMDRRAVALERQLNGGVDFLSSVNNYFQSVMAEHRENKTGNKILMEKINSCVFGTDSN
                     HFSCPESFLTCPITLDTPETGVFMRNSRGAEICSLYDKDALVQLVETGGTHPL"
BASE COUNT          142 a           73 c          119 g          135 t
ORIGIN      
        1 ccagacttga aagtggaata agaatatcca ttggtagtgg ccaatattct gttcactatg
       61 tccaactact ggatggattt tcagttgaac cggtgagagg aggcttactg gataggctat
      121 tggggcgtga gcatcgaatg gatagaaggg ctgtggctct ggaaaggcaa ttaaatggag
      181 gtgtcgattt tttaagtagt gttaataact attttcagag tgtcatggca gaacacagag
      241 aaaataaaac aggtaataaa atattaatgg aaaaaataaa ttcttgtgta tttggaacgg
      301 attctaatca cttttcttgc ccggagtcat ttttgacatg cccgataacg ctggacacac
      361 ctgagactgg agtgttcatg agaaactcac gaggtgctga gatatgctct ctatatgata
      421 aggatgcgtt agtgcaactt gttgaaactg gtggaactca tcctctgag
//
LOCUS       DAZKIF010000475          467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZKIF010000475 DAZKIF010000000
VERSION     DAZKIF010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.479"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..111
                     /locus_tag="ACHOTW_005277"
     CDS             <1..111
                     /locus_tag="ACHOTW_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HFP9847158.1"
                     /translation="EFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(136..>467)
                     /locus_tag="ACHOTW_005278"
     CDS             complement(136..>467)
                     /locus_tag="ACHOTW_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847159.1"
                     /translation="RFQFIIYTLTSWSILITLNDINLALSFILVINNTYVNINIGAKR
                     IRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWILFTTIMLSAIPSA
                     KMSLSAH"
BASE COUNT          147 a           87 c           93 g          140 t
ORIGIN      
        1 gagtttatgc cagaactgaa gtggagtttc ggctacccag gcgcgattat ctttatgatc
       61 ctcgcgggcc tggcaccgta tctgtacttt aagcgcaaga actggctgta actatacgat
      121 gtcgctgtga gtaagttagt gtgctgacag cgacatttta gcagagggaa tcgcagataa
      181 cataattgtt gtgaataata tccatgccac gttgagttgg acgcttaatg actcacatct
      241 caccccttca attgctgcag agataataga aaaaagtgtc aaacccaatg aaatcaatac
      301 aaagtagatt gccttacatc cagtatctct tattcttttt gctccaatat tgatatttac
      361 ataggtatta ttaataacca gaataaaact caaagccagg ttgatatcat tcaaagttat
      421 taatattgac catgatgtta atgtatagat tatgaactga aagcgtg
//
LOCUS       DAZKIF010000476          464 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZKIF010000476 DAZKIF010000000
VERSION     DAZKIF010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.480"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>464
                     /locus_tag="ACHOTW_005279"
     CDS             <1..>464
                     /locus_tag="ACHOTW_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9847160.1"
                     /translation="NKKTKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTHL
                     ACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEALEQIGQ
                     LYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSRHSELAKAF"
BASE COUNT          112 a          115 c          149 g           88 t
ORIGIN      
        1 gtaataagaa gacgaagacc gggcggttgt gggcgtatgt tcgtgatgac cgcaatgcag
       61 ggtcagcgtt ggcacctgca gtgtggttcg cttacagccc ggacagaaaa ggcatccatc
      121 cgcagactca tcttgcctgc ttcagcggtg tgctgcaagc ggatgcgtac gccgggttca
      181 acgagctgta tcgcaatggt gggataacgg aagctgcctg ctgggctcat gcccgccgaa
      241 agatccacga tgtgcacgtc cgcatcccgt cagcactgac ggaagaagcc ctggagcaga
      301 tcggtcagtt gtacgccata gaggcggata taaggggaat gccggcagag cagcggcttg
      361 ctgaacgtca gcgaaaaacg aaaccgttgt tgaaatccct ggaaagctgg ttgcgtgaaa
      421 agatgaagac cctgtcgcga cactcagagt tggcgaaggc gttc
//
LOCUS       DAZKIF010000477          463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZKIF010000477 DAZKIF010000000
VERSION     DAZKIF010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.481"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>463)
                     /locus_tag="ACHOTW_005280"
     CDS             complement(<1..>463)
                     /locus_tag="ACHOTW_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFP9847161.1"
                     /translation="LGDLTGVNQVSFRRLRETSSTRNQLNKILRQEIHDKISGVELSD
                     INKEGFLYQSIGKIRLLALSSALFDIQCPDYIFSRLYRETLIREIGYQNVKQLSFYWQ
                     GGQCKPEYGEERFCAELIKYGAGNLEWLFADNPLWTIVKYLLPKSGEIKPTH"
BASE COUNT          145 a          100 c           64 g          154 t
ORIGIN      
        1 atgagttggt tttatttctc cacttttcgg caaaagatat ttgactatgg tccataaagg
       61 attatcagca aatagccact ccaaatttcc tgcaccgtat tttattaact cagcacaaaa
      121 ccgttcttca ccatattctg gtttacattg ccctccctgc caatagaatg ataactgctt
      181 cacattttga tatcctatct ctctaatcag agtttctcga tataatcgtg agaaaatata
      241 atcagggcat tgaatgtcaa ataatgcact acttagagca agcaatctta ttttcccaat
      301 tgactgatat aaaaaacctt ccttatttat gtcagataat tccacaccag aaattttatc
      361 atgtatttcc tgacgcaata tcttattgag ttgattacga gtagaactag tttctcttaa
      421 acgccgaaaa ctgacctgat tgacaccggt taaatcacct agc
//
LOCUS       DAZKIF010000478          463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZKIF010000478 DAZKIF010000000
VERSION     DAZKIF010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.482"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..203)
                     /locus_tag="ACHOTW_005281"
     CDS             complement(<1..203)
                     /locus_tag="ACHOTW_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9847162.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
     gene            complement(355..>463)
                     /locus_tag="ACHOTW_005282"
     CDS             complement(355..>463)
                     /locus_tag="ACHOTW_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9847163.1"
                     /translation="QFNPTESVTIDLAYEGSGSGDWRSDAFIVGIGYRF"
BASE COUNT          123 a          129 c           96 g          115 t
ORIGIN      
        1 ggaaatccct ccaccaacag aatgacgctg tactgaccgt actcaacatc catgctgtaa
       61 cgtccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt gctgctggtt
      121 cgtctggcct tcagcacaat ggtgcagttc tgtactggtt ttcctgtgcc atctttaagc
      181 acgccagaaa tttttactgt catacttttc caccaataaa aaaagcccgc agcagtgacg
      241 ccacgggctt caggacagtg taactttacg tttcctcaaa cgcagttcac cccataaggt
      301 ggatgaacct gcgtatcata acaatattta cagaagataa atcggcgtct gttgtcagaa
      361 acggtatccg ataccaacaa taaatgcatc cgatcgccag tcgccactac cggaaccttc
      421 ataagcaagg tcaatggtca cggattcggt cgggttaaac tgc
//
LOCUS       DAZKIF010000479          456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZKIF010000479 DAZKIF010000000
VERSION     DAZKIF010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.483"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(24..371)
                     /gene="tnpB"
                     /locus_tag="ACHOTW_005283"
     CDS             complement(24..371)
                     /gene="tnpB"
                     /locus_tag="ACHOTW_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9847164.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          101 a          132 c          128 g           95 t
ORIGIN      
        1 tgtgacaacc aggataaaga gatttacagc atggtcaggg aggtcagcag ccgcttaggc
       61 tgtcgccagt cgataccttc cagcagcatc gccagctgcg cctgcgtaag gaacactttg
      121 ccatcacggg ctgacggcca ggcgaagcgc ccacgctcca gccgtttggt caggaggcac
      181 agtccgtcac cggtggacca cagcagttta acctgactgc cgctgcggcc ccggaaaatg
      241 aaaacatggc cggacatggg atcgtctttc agcgccgttt gtactttcgc agccaggccg
      301 ttgaagccat ttctcatatc ggtgataccg gcaaccagcc aaattttggt cccggaaggt
      361 aacgggatca tcgcttcagt tcctgtatca gcagagtcag gagcttttcg ctgacattgc
      421 cattgaagcg gagcgtcccg tgccggaacg ttacct
//
LOCUS       DAZKIF010000480          455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZKIF010000480 DAZKIF010000000
VERSION     DAZKIF010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.484"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>455
                     /locus_tag="ACHOTW_005284"
     CDS             <1..>455
                     /locus_tag="ACHOTW_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847165.1"
                     /translation="LDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNY
                     VKLCLSGYTPANAHFKDIFDGVYEFIRNIRKNSTPGKTKLIATINTCKETCKHLLYLS
                     NEDKEKIISDLDKVQVACYYLTILLAFERRTSLTSTLATLYKMLISERE"
BASE COUNT          165 a           86 c           75 g          129 t
ORIGIN      
        1 cactcgacat caggaacaca tatcgcccgg ataatgagat ggcattttac tatcatattc
       61 attcttttct cactcagata ccggataatg aggatgatat atatcatgct gcaaggacat
      121 atctgcgaaa ttacgttaag ttatgtttat ccggatacac gccagcgaat gcgcatttca
      181 aagatatctt tgatggcgta tatgaattca ttcgtaatat tcgcaaaaac agtacaccag
      241 gaaaaacaaa acttatcgca actatcaaca catgcaaaga aacctgtaaa catctgcttt
      301 atttaagtaa tgaagacaag gaaaaaataa tttctgactt agataaagtt caggttgcat
      361 gttattatct cactatatta ctggctttcg aaagacgaac ttcattaaca agcaccctgg
      421 caactttata taaaatgctg ataagcgaaa gagaa
//
LOCUS       DAZKIF010000481          451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZKIF010000481 DAZKIF010000000
VERSION     DAZKIF010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.485"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..103
                     /locus_tag="ACHOTW_005285"
     CDS             <1..103
                     /locus_tag="ACHOTW_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9847166.1"
                     /translation="QQQNEQLYPAQEHLLLQLCMRVTKKLTDILMPP"
     gene            133..417
                     /locus_tag="ACHOTW_005286"
     CDS             133..417
                     /locus_tag="ACHOTW_005286"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9847167.1"
                     /translation="MLLALLSSTDNFCLSSTELSERLDVSRTYITRACDSLEKFGFIK
                     RMESKEDRRSKNIYLTSDGNLYLQRTTRIYGRYLKKYGATLQMMKSKHLK"
BASE COUNT          161 a           76 c           77 g          137 t
ORIGIN      
        1 tcaacaacag aatgagcaat tatatccagc acaggaacat cttcttttac aactttgtat
       61 gcgagttacc aaaaaactta ctgatatatt aatgcctccc tgaaggggga tggtataaac
      121 gatacaacgc ttatgcttct agccttgtta tccagtactg ataatttctg cttatcttcg
      181 actgaactca gtgaaagatt agatgtttcc agaacatata tcaccagagc ttgtgattct
      241 ctggaaaaat ttggttttat taaacgtatg gaaagtaagg aagatcgacg tagtaagaat
      301 atttatctga cttcagacgg taatttgtat ttacagcgga ccacaaggat atatggacgc
      361 tatttaaaaa aatatggagc aaccttacag atgatgaaat caaaacattt aaaataatca
      421 gcaaaaaggt tttatctcaa aatatttctc a
//
LOCUS       DAZKIF010000482          449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZKIF010000482 DAZKIF010000000
VERSION     DAZKIF010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.486"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>449
                     /locus_tag="ACHOTW_005287"
     CDS             <1..>449
                     /locus_tag="ACHOTW_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847168.1"
                     /translation="MIFSAIKEIYDEDPSFIFDKISHKLRHTVTEFDESGKSEPTGLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDGYHIQKGFRIDGDNLTQYCSKKARS
                     ASAWFEHSKAIIAESFATGSDHQVVNELKGERLRELNEAFNPSNLARG"
BASE COUNT          166 a           79 c           90 g          114 t
ORIGIN      
        1 atgatcttca gcgcaataaa agaaatatat gatgaagatc caagttttat cttcgataaa
       61 atatcacaca aactcagaca tacggtaacg gaattcgatg agagcgggaa aagcgaacca
      121 acgggcttat ttacctggta cggtaaagat aaaaaaggcg actctctcgc tattgtaatt
      181 aaaaataaaa atggaaatga ttacttatct ctcggttact acgatcagga tggctaccac
      241 attcaaaaag gatttcgtat tgatggtgat aatctcaccc aatattgtag taaaaaagcc
      301 aggagtgctt ctgcgtggtt tgaacacagt aaagcaatca ttgcagaatc atttgcaaca
      361 ggttccgatc atcaagttgt aaacgaactc aaaggggaaa gactgagaga acttaacgaa
      421 gcttttaatc cttcaaatct agcaagagg
//
LOCUS       DAZKIF010000483          448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZKIF010000483 DAZKIF010000000
VERSION     DAZKIF010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.487"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          122 a           83 c          109 g          134 t
ORIGIN      
        1 cataataaaa aaagagccag cgactgacct gaaagaagac gctggctaaa aggccttata
       61 tgtttgtaga gacttatttt tcacagacag caatgatgcc tgtcaatata ttatcaatat
      121 gcggattgtt tcagttacag atgctttatt aaggaaaaaa acagccagca ctgactttcg
      181 gtggagaggt gctggctcaa aaggatagat gtacttcaca tgttgcttct atatggcagt
      241 acattttctg acagacagtg acggatgttg tcaagatatt gtgtcattta taacctgaat
      301 cgggggaggc cggaatgtta tctggcattt ttagcagagc ctgaatgcca taatcacggc
      361 tcccggcgtt ggccgtcagt gggtgacact ggcggctttt ttgttttcct ttactttcat
      421 tttctgttgg cggtgacgga gacataca
//
LOCUS       DAZKIF010000484          443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZKIF010000484 DAZKIF010000000
VERSION     DAZKIF010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.488"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          173 a           95 c           51 g          124 t
ORIGIN      
        1 gtgatcatgt agcataagta acctccgttt atctacagac tgccatcctg aatttaccgt
       61 ccttaataag acaacaataa aaaactaatt atataaaaac aataaaaata atatacaaaa
      121 aaactaaaca acaaagaaaa gcaaatcata tctggcattt taattgaaag agcaattacc
      181 gaacaaaaga cgctgactat atactcaaaa ccaaacaaca attctgccaa tcaggtatca
      241 tggcaacaca cggaattacc atgtttttgc cttctctgcc catacaatac gggcatatac
      301 ttcatactct attgtaatat ttctatccat gtgccccact ccatttacct gtaaataata
      361 ttcaaaatat ttatcacaga aatcgttttt ggccatgaac tgagcacact ataaagtccg
      421 gaactgactc tttgttaaat tac
//
LOCUS       DAZKIF010000485          442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZKIF010000485 DAZKIF010000000
VERSION     DAZKIF010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.489"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(47..151)
                     /locus_tag="ACHOTW_005288"
     CDS             complement(47..151)
                     /locus_tag="ACHOTW_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847169.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(267..>442)
                     /locus_tag="ACHOTW_005289"
     CDS             complement(267..>442)
                     /locus_tag="ACHOTW_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9847170.1"
                     /translation="DGQHVIILCDGAFVLYAQYRDGEFFDVVRNGDEFFETQSRNVTD
                     WMPLPEPPQEVRQ"
BASE COUNT          137 a          123 c           77 g          105 t
ORIGIN      
        1 gcaaaccgtt tttacccgat atgggaattc ccatatcgta atgaattcag ttccctagtc
       61 gatccatcaa aaacacaacc aggcagtaaa cgcccacaac agcaataaca gccagcgcac
      121 cttccattgc cagtgaaata tcatccgaca tattccctcc tttggtgtga atcccggcga
      181 acgtttttac ccccaccgac aaataacata tactaaaaaa tcaatagcta tagcaacgcc
      241 tgtaattgca aaggcttcag gccagatcat tggcgcacct cctgcggcgg ttctggtagc
      301 ggcatccagt cggttacatt gcggctctgt gtttcgaaaa attcatcacc attacggact
      361 acatcaaaaa actcaccgtc tcgatattgc gcataaagaa cgaatgcgcc atcacataaa
      421 ataattacgt gctgaccatc at
//
LOCUS       DAZKIF010000486          441 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZKIF010000486 DAZKIF010000000
VERSION     DAZKIF010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.490"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            322..441
                     /locus_tag="ACHOTW_005290"
                     /pseudo
     CDS             322..441
                     /locus_tag="ACHOTW_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          118 a          114 c           78 g          131 t
ORIGIN      
        1 aataaaaaac agcttcaggt aatgaggaat atctcaattt ttaaacataa aatgcgaatt
       61 atttagtaca aaaagtgaac ttttgatgat ctttccctaa tctcacatag gatcagatct
      121 cgtctgatcg ttccccctgc gctcactacc atggctcttt accacagcag ccggggttgt
      181 ttgggaccga ctttcgacag cagatcccgg tgggcctgcc accatctctt cacaagctta
      241 tgcttattac atgcaaaacg tcccgttttg catctttgtg cctacggcac accgatgatc
      301 tttccctgtg atctttccct gaatctgccc ctgagaacct gaatcaaacg tctgactcgc
      361 agaaccatct gtttctcgcg ctctgtagaa cttcatgaaa aagtcatcgg tgcttttatc
      421 gaaaaataca tgttctacta a
//
LOCUS       DAZKIF010000487          437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZKIF010000487 DAZKIF010000000
VERSION     DAZKIF010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.491"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            67..143
                     /locus_tag="ACHOTW_005291"
     tRNA            67..143
                     /locus_tag="ACHOTW_005291"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:101..103,aa:Arg,seq:tct)
     gene            317..>437
                     /locus_tag="ACHOTW_005292"
     CDS             317..>437
                     /locus_tag="ACHOTW_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9847171.1"
                     /translation="MVFLVKKARRAATEIKKFEKRDLAQVVINAAYLVACADGE"
BASE COUNT           95 a          116 c          117 g          109 t
ORIGIN      
        1 acgccagcct tcgaagctgg tttcgcgggg ttcgagtctc cgatggcggt ccattatcgg
       61 tattctgcgt ttttagctca gccggacaga gcaattgcct tctaagcaat cggtcactgg
      121 ttcgaaccca gtacaacgca ccacacttat tttccctcgc tcgcttttgc ggaccttttt
      181 tgtatccgca ccacgcccgg cgcataccaa ccacagagcc tttcgggggg agcttatgga
      241 gtggtcagtg tgactttctc tgtgggcaga tcgctcccgg gcgttggctc acccacccaa
      301 aggaacgtca cgatgtttgg tatttttggt aaaaaaagcc cgcagagcgg caacggaaat
      361 taaaaagttt gaaaaacgcg atctggcaca ggtggtgatt aacgccgcat acctggtggc
      421 ctgtgcagat ggtgaat
//
LOCUS       DAZKIF010000488          433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZKIF010000488 DAZKIF010000000
VERSION     DAZKIF010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.492"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>433)
                     /locus_tag="ACHOTW_005293"
     CDS             complement(<1..>433)
                     /locus_tag="ACHOTW_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847172.1"
                     /translation="LSSLQDKEDDVDELKYSAEDFDSLTVADLYDIEIAMQDFLNDIN
                     FENSKDNKVRFDEDTYDFNINGKRRGMFGKGTRAVMHAIFTICFAEFLSRKGNPFIGF
                     VVLDSPLVTHFDKDRGGSLSDVNSVSLSDSFYHALIKRDYNF"
BASE COUNT          140 a           88 c           64 g          141 t
ORIGIN      
        1 gaaattatag tcccttttga ttaatgcgtg ataaaatgaa tcactgagac tgacgctatt
       61 aacatccgat agagaacccc ctctatcttt atcaaagtgc gtcactaatg gactatcaag
      121 cacaacaaat ccaatgaatg gattcccttt tcttgataaa aattccgcaa agcaaatggt
      181 aaatatagca tgcattactg cacgggtacc tttaccaaac attcctcggc gtttcccatt
      241 gatattaaag tcatatgtat cttcgtcaaa tcgcactttg ttatctttag aattttcaaa
      301 atttatatca ttcaaaaagt cttgcatcgc tatttctata tcatacaaat cagctactgt
      361 taaggagtca aagtcctcgg cggagtattt aagttcgtca acatcatctt ctttatcttg
      421 taatgaagat aga
//
LOCUS       DAZKIF010000489          430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZKIF010000489 DAZKIF010000000
VERSION     DAZKIF010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.494"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..267
                     /locus_tag="ACHOTW_005294"
     CDS             <1..267
                     /locus_tag="ACHOTW_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP9847173.1"
                     /translation="LTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAA
                     LRISISRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            264..>430
                     /locus_tag="ACHOTW_005295"
     CDS             264..>430
                     /locus_tag="ACHOTW_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP9847174.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTG"
BASE COUNT          120 a          103 c          134 g           73 t
ORIGIN      
        1 cttaccgaag cgcagcaact gactgctgaa gagcaggtcg aaaaatgggt ggatggcagg
       61 aaaaaaatac tgtgggacag caaaaagcga cgcaatgagg cgctcgactg cttcgtttat
      121 gcgctggcgg cgctgcgcat cagtatttcc cgctggcagc tggatctcag tgcactgctg
      181 gcgagcctgc aggaagagga tggtgcagca accaacaaga aaacactggc agaatacgcc
      241 cgtgccttat ccggagagga tgaatgacgc gacaggaaga acttgccgct gcccgtgcgg
      301 cactgcatga cctgatgaca ggaaaacggg tggcaacggt acagaaagac ggacggcgag
      361 tggagtttac gaccacttcc gtgtctgacc tgaaaaaata cattgctgag ctggaagtgc
      421 agaccggcat
//
LOCUS       DAZKIF010000490          430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZKIF010000490 DAZKIF010000000
VERSION     DAZKIF010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.495"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            118..>430
                     /locus_tag="ACHOTW_005296"
     CDS             118..>430
                     /locus_tag="ACHOTW_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HFP9847175.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LG"
BASE COUNT          125 a          104 c          100 g          101 t
ORIGIN      
        1 ggtattatta atcgcagtga taggatattg cacagctatt gcgcgatatc atcagaacgc
       61 tgtttgttac cctgtaacca gcaagctcag tctgttaacg gaattaatga gggttttatg
      121 aaatgtaaaa tcattgctgc cattgccatg ctgacagcag catcatgcgg atacgcagca
      181 gaacaggaag tcccaatgaa ccttgtcagt gctgacggaa aagaagtcag cattggaaaa
      241 ataaccattc aggagacccc ctacggtctg ctgttcacac cagcccttca ctctctgtct
      301 gaaggcattc atggttttca tgtgcacgaa aaaggaaatt gcgccccggc actgaaagac
      361 ggaaaaccgg tcgcagcatt atcggctggc ggtcactttg acccgaaaaa caccggcaaa
      421 catcttggcc
//
LOCUS       DAZKIF010000491          430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZKIF010000491 DAZKIF010000000
VERSION     DAZKIF010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.496"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            39..>430
                     /locus_tag="ACHOTW_005297"
     CDS             39..>430
                     /locus_tag="ACHOTW_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847176.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRR"
BASE COUNT          131 a           79 c           83 g          137 t
ORIGIN      
        1 tcatagtaaa gtagaacact ttttatatga gttactatat gaaaatatac tggacaagaa
       61 aacgtattcc tgaactttca gcacttccac cttcattaag aaagaaaaat tttactgacg
      121 catataatgc agcatcttct catatcgaat actggatagg tgctggcgtc tcatttatct
      181 cgatgatgat tttatccaga gtatatgact ttcttttacc cgcccaagat actttcccgg
      241 gtgatatcat cagaagttta tgtgttgtgt gtccatccat attaatctgg tttcagtttt
      301 cggtatatgt catgaggaaa tattacagac atattctggt gcgaggaaaa gaaaccgaaa
      361 ccatttcaga aaggttgata cgtgaggctg atacacgaga atatgaacta tggcgtccgg
      421 tccgtcgctt
//
LOCUS       DAZKIF010000492          428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZKIF010000492 DAZKIF010000000
VERSION     DAZKIF010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.497"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>428
                     /locus_tag="ACHOTW_005298"
     CDS             <1..>428
                     /locus_tag="ACHOTW_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFP9847177.1"
                     /translation="NKYYLFSRRANDKIVVYHQEQVNGPLVDESGQVIFADFNPSKRP
                     WSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEY
                     LDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGDYN"
BASE COUNT          132 a           80 c           92 g          124 t
ORIGIN      
        1 ataataaata ttatctcttt tcgcggaggg cgaacgataa aatcgttgtt tatcatcagg
       61 aacaagtaaa tggaccgctt gtcgacgagt cagggcaggt tatttttgcc gattttaacc
      121 catcgaaacg accgtggtcg gtggcttcag atgactctaa caacagctgg aatccggcct
      181 acaattgctt tgatcgtccg ggtaaaaaat gtatctcttt tacgctacgc atcaacggca
      241 aagatcacga tttgttagcg gtggataaaa ttcatgtcga tttaaactgg cgatatctga
      301 acgagtatct tgatcaaatc agcgctaatg atgaagttct atttttgaaa caaggccatg
      361 agatcattgc caagaatcaa ctcgctcgtg aaaaactgat tatttataat agcgaaggtg
      421 attataat
//
LOCUS       DAZKIF010000493          425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZKIF010000493 DAZKIF010000000
VERSION     DAZKIF010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.498"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..167
                     /locus_tag="ACHOTW_005299"
     CDS             <1..167
                     /locus_tag="ACHOTW_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847178.1"
                     /translation="EEQAEWLIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRP
                     SRTFQQRIWR"
     gene            169..>425
                     /locus_tag="ACHOTW_005300"
     CDS             169..>425
                     /locus_tag="ACHOTW_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847179.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVIRAAGGAGALSDW"
BASE COUNT           91 a          118 c          120 g           96 t
ORIGIN      
        1 ctgaagaaca ggccgaatgg ctcattcagt gttaccgcag gcgcggatgc gaggttaaaa
       61 aagcccttag cctcgactac cgtcactgga taatctccgt caggctccct tactccgaac
      121 ggccaccgcg tccgtcccgc acattccagc aacggatctg gaggtaatgt gcgggtatta
      181 cttcgacctg ttctggtacc ggaactcggt ctggttatcg ttaagccagg ccgtgaatca
      241 atgtcagcat tccataacgg cagaatactg gtggagccgg aaccaaaaag catgcgagct
      301 ctgccgtccg gggttgtacc tgccgttcac cagccgctgg cggaagataa atcactactg
      361 ccatttttca gcgatgagcg ggtgatccgt gctgcgggtg gcgctggtgc actgtctgac
      421 tggtt
//
LOCUS       DAZKIF010000494          424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZKIF010000494 DAZKIF010000000
VERSION     DAZKIF010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.499"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>424
                     /gene="wzx"
                     /locus_tag="ACHOTW_005301"
     CDS             <1..>424
                     /gene="wzx"
                     /locus_tag="ACHOTW_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFP9847180.1"
                     /translation="QERYRIISLSVISSLISKILSLLSLILTVSLTLPYLGQERFGVW
                     MTITSLGAALTFLDLGIGNALTNRIAHSFACGKNLKMSRQISGGLTLLAGLSFVITAI
                     CYITSGMIDWQLVIKGINENVYAELQHSIKVFVIIFGLG"
BASE COUNT          128 a           62 c           87 g          147 t
ORIGIN      
        1 caggaaaggt acaggattat atccttgtct gttatttcaa gtttgattag taaaatactc
       61 tcactacttt ctcttatatt aactgtaagt ttaactttac cttatttagg acaagagaga
      121 tttggtgtat ggatgactat taccagtctt ggtgctgctc tgacattttt ggacttaggt
      181 ataggaaatg cattaacaaa caggatcgca cattcatttg cgtgtggcaa aaatttaaag
      241 atgagtcggc aaattagtgg tgggctcact ttgctggctg gattatcgtt tgtcataact
      301 gcaatatgct atattacttc tggcatgatt gattggcaac tagtaataaa aggtataaac
      361 gagaatgtgt atgcagagtt acaacactca attaaagtct ttgtaatcat atttggactt
      421 ggaa
//
LOCUS       DAZKIF010000495          423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZKIF010000495 DAZKIF010000000
VERSION     DAZKIF010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.500"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..380
                     /gene="espW"
                     /locus_tag="ACHOTW_005302"
     CDS             <1..380
                     /gene="espW"
                     /locus_tag="ACHOTW_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFP9847181.1"
                     /translation="NVIKLSQLSIRYLPNISSISLIETKQSLLLHRLYSDEVLQRNGT
                     LIPTPLHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDI
                     LACSPRNYGLHHNIKTWDPLVRN"
BASE COUNT          163 a           87 c           66 g          107 t
ORIGIN      
        1 ggaatgtaat aaaactcagt cagttatcaa ttcgttactt acccaatatc agctcaatct
       61 cattaataga gacaaaacag agtttgttgc tacatcgatt gtattcagat gaagtacttc
      121 agagaaatgg aacgcttatc ccgacaccac tacatgaaga aaaatcaatt ccagctgaca
      181 atataaaaac aatgctcaac aacataccga cttacaaaat gttaccgcca ttcacagaaa
      241 cacaaggtaa ttgttcttct ggcgcagcca cgtttttacg caaatcaggc gccgaagaaa
      301 aagatattct tgcatgtagc ccccgaaatt atgggctgca tcataacata aaaacatggg
      361 accccttggt tagaaattaa ggatccaaat attatatatt tcatataaag caagaaaaaa
      421 ata
//
LOCUS       DAZKIF010000496          418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZKIF010000496 DAZKIF010000000
VERSION     DAZKIF010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.501"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..353
                     /locus_tag="ACHOTW_005303"
     CDS             <1..353
                     /locus_tag="ACHOTW_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847182.1"
                     /translation="IHSAMINNLLQEVDIALGKINDIIDSRNISAPHELANILTREKI
                     LTTREKKGNLISLFDGFTLFHCVGMITFLIHYLRTPEEKVENIFMLYGADKNNKLRRR
                     LIYDALGIIQSQQE"
BASE COUNT          162 a           71 c           70 g          115 t
ORIGIN      
        1 acattcactc agctatgata aataacctat tacaggaagt tgatatagcc cttggtaaaa
       61 taaatgacat tatagacagc agaaacatat cagcccccca tgaactggca aatattctta
      121 cccgcgaaaa aatacttaca acacgggaaa aaaaaggaaa cctgataagc ctgtttgatg
      181 gattcacttt attccattgt gttggaatga taacctttct tatccattat ctcagaacac
      241 ctgaagaaaa agttgaaaat atatttatgt tatatggtgc agataaaaac aataaactac
      301 gcagaagact gatttatgac gcactaggaa taattcagtc tcagcaggag tgaagagtta
      361 aacaggcaaa ttatttttta taaaaagggg attgttaagt aatcccctgt taatcaaa
//
LOCUS       DAZKIF010000497          418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZKIF010000497 DAZKIF010000000
VERSION     DAZKIF010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.502"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>418
                     /locus_tag="ACHOTW_005304"
     CDS             <1..>418
                     /locus_tag="ACHOTW_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFP9847183.1"
                     /translation="DSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVG
                     EEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGT
                     IKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRT"
BASE COUNT          102 a          102 c          112 g          102 t
ORIGIN      
        1 tggattccta cattccggaa ccagagcgtg cgattgacaa gccgttcctg ctgccgatcg
       61 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggta
      121 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
      181 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg ccgtgctggt gagaacgtag
      241 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
      301 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
      361 aaggcggccg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtac
//
LOCUS       DAZKIF010000498          412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZKIF010000498 DAZKIF010000000
VERSION     DAZKIF010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.503"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>412
                     /locus_tag="ACHOTW_005305"
     CDS             <1..>412
                     /locus_tag="ACHOTW_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9847184.1"
                     /translation="GETVRYRYDDAYSELPATTTDATGSTRQMTWSRYGQLLAFTDCS
                     GYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLISVKDAQGHETRYEYNAAGDLTA
                     VITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           97 a          123 c          133 g           59 t
ORIGIN      
        1 gcggggagac agtacgctac cgctacgatg atgcgtacag tgagttaccg gcgacgacaa
       61 cagatgcgac gggcagcacc cggcagatga cctggagccg ctacggtcag ttgctggcgt
      121 tcaccgactg ctcgggctac cagacccgct atgaatacga ccgcttcggc cagatgacgg
      181 cggtccaccg cgaggaaggc atcagccttt accgccgcta tgacaaccgt ggccggttaa
      241 tctcggtgaa agacgcacag ggccatgaaa cgcggtatga gtacaacgcc gcaggcgacc
      301 tgactgccgt tatcaccccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
      361 aggcggtcag caccacgcag ggcgggctga cgcgcagtat ggaatacgac ct
//
LOCUS       DAZKIF010000499          412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZKIF010000499 DAZKIF010000000
VERSION     DAZKIF010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.504"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>412
                     /locus_tag="ACHOTW_005306"
     CDS             <1..>412
                     /locus_tag="ACHOTW_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9847185.1"
                     /translation="GDVIRYAYDNPHSELPATTTDATGSTRQMTWSRYGQLLAFTDCS
                     GYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGRLTSVKDAQGHETRYEYNAAGDLTA
                     VITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           97 a          117 c          131 g           67 t
ORIGIN      
        1 gtggggacgt catccggtat gcttatgata atccgcacag tgaattaccg gccacgacaa
       61 cagatgcgac gggcagcacc cggcagatga cctggagccg ctacgggcag ttgctggcgt
      121 tcaccgactg ctcgggctac cagacccgtt atgaatacga ccgctttggt cagatgacgg
      181 cggtccaccg tgaggaaggt atcagccgtt accgccgcta tgacaaccgt ggccggttaa
      241 cctcggtgaa agacgcacag ggccatgaaa cgcggtatga gtacaacgcc gcaggcgacc
      301 tgactgccgt tatcactccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
      361 aagcggtcag caccacgcag ggcgggctga cgcgcagtat ggagtatgac ct
//
LOCUS       DAZKIF010000500          407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZKIF010000500 DAZKIF010000000
VERSION     DAZKIF010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.505"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..251
                     /locus_tag="ACHOTW_005307"
     CDS             <1..251
                     /locus_tag="ACHOTW_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9847186.1"
                     /translation="QFFRLLNRRYEKASIILTSNKGFADWGEMFGEHVLATAILDRLL
                     HHSTTLNIKGESYRFNIPDRLVVHLSLHARPELRAHNT"
     gene            complement(244..407)
                     /locus_tag="ACHOTW_005308"
                     /pseudo
     CDS             complement(244..407)
                     /locus_tag="ACHOTW_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           98 a          113 c          105 g           91 t
ORIGIN      
        1 ttcagttctt ccggctactg aaccgtcgat atgaaaaagc gagcatcata ctgacgtcaa
       61 acaaagggtt cgcagactgg ggagaaatgt tcggagagca tgtgctggca acagcgatcc
      121 tggaccggct gctacatcac tcaaccacgc tgaatatcaa aggagagagt taccggttca
      181 atattccaga cagactcgtg gttcatttga gcctccatgc tcgccctgaa ctccgggcac
      241 acaatacgtg atgtctcccg cccacttctg gttcgggcca ctggcgtaaa aatcctgctc
      301 cagcagattt tctgacacag gctggctgtg tgcgcggtaa cagaccggac tgaaattcca
      361 ggaagctttt gccctcagtc cgtgacggcg caggctttcc gtcacgg
//
LOCUS       DAZKIF010000501          401 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZKIF010000501 DAZKIF010000000
VERSION     DAZKIF010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.506"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>401
                     /locus_tag="ACHOTW_005309"
     CDS             <1..>401
                     /locus_tag="ACHOTW_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847187.1"
                     /translation="LEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEI
                     MKFVMNNPEGERADSPKVIIEISLSTITTMGTRQGHTAIIFPQPDGSTNRYERKSFER
                     KDESSLHLITNKVLACYQREANKEIARLLNN"
BASE COUNT          135 a           69 c           83 g          114 t
ORIGIN      
        1 ttggagtttt tggtaaaaat atttaccctg ggttatgtgg attttagcaa acgctccaac
       61 gaagcgggaa gaaatatgat ggctcatatt gagtcctcat cttatatcaa aaataatgat
      121 ggcagtgaga taatgaagtt tgttatgaat aatcctgaag gggaacgagc ggattcaccc
      181 aaggtgatta tagaaatttc actttccact attactacta tggggactcg tcaaggacat
      241 acagccatta tatttccaca acctgatggt tcgactaacc gttatgaaag aaagtccttt
      301 gaaagaaaag atgagagttc attacacctg attactaaca aggttctggc gtgttaccaa
      361 cgcgaagcta acaaggaaat agctcgtcta ttaaataatc a
//
LOCUS       DAZKIF010000502          398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZKIF010000502 DAZKIF010000000
VERSION     DAZKIF010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.507"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>398)
                     /locus_tag="ACHOTW_005310"
     CDS             complement(<1..>398)
                     /locus_tag="ACHOTW_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFP9847188.1"
                     /translation="KGDAYANPLSDDDWQRLAKVKDLTPGEMTAEPYDDNYLDDEDAD
                     WTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWV
                     SSIGKAVTAKEVITRTVKVTNVGKPSVAEE"
BASE COUNT           71 a          137 c           86 g          104 t
ORIGIN      
        1 ttcttccgcc acggaaggtt tgcccacgtt agtgactttc accgtgcggg tgatcacttc
       61 tttcgccgtc acggccttac cgatactgct gacccagcca cggaacacat ccaccgtgcc
      121 atttgggaaa cggattttat aggcccgcac atccccgctt tcaaaccagc ctataagccc
      181 tttctgacct tcttctcccg gtttccaggc cagcgtaaaa ctggtatctc ctgcagactt
      241 ctgcccctgc ccggtcgcgg tccagtccgc gtcttcatca tccaggtagt tatcatcgta
      301 gggttctgcc gtcatctcgc ccggcgtcag atccttcacc ttagccagtc gctgccagtc
      361 atcgtctgac aacgggtttg cataagcatc acccttgc
//
LOCUS       DAZKIF010000503          392 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZKIF010000503 DAZKIF010000000
VERSION     DAZKIF010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.508"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..362
                     /locus_tag="ACHOTW_005311"
     CDS             <1..362
                     /locus_tag="ACHOTW_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9847189.1"
                     /translation="PEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKP
                     VSKGVSHAAPGGTASRAVQEIRAAREQWERENGFISNGNGLEAVGTYGGGVFEPLDPE
                     ERGCAVEALDCSDWRDD"
BASE COUNT           97 a           92 c          132 g           71 t
ORIGIN      
        1 agcctgagtt tacacctgct gagcttgccg cattccggga ttactggatg gcggagggga
       61 aggttttcac gcaggttcag tgggagcaga aatttgcccg tcacgtgcag cacgtcaggg
      121 cacaggtaaa accagtcagc aagggggtaa gccatgcagc accaggtggc accgcatcac
      181 gggcagttca ggaaattcgg gcagcacgtg aacagtggga acgtgaaaac ggatttatca
      241 gcaacggaaa cggcctggaa gctgtgggaa cttatggggg aggtgtattc gaaccgctgg
      301 acccagaaga acggggctgc gccgtcgaag ctctggattg ctcagattgg cgcgatgact
      361 gaacagcaaa tccggcaggt ctgccgtcag tg
//
LOCUS       DAZKIF010000504          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZKIF010000504 DAZKIF010000000
VERSION     DAZKIF010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.509"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..337
                     /locus_tag="ACHOTW_005312"
     CDS             <1..337
                     /locus_tag="ACHOTW_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9847190.1"
                     /translation="YPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSG
                     RLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVRGQLVPGERLG
                     IKITELECA"
BASE COUNT           97 a           94 c          106 g           89 t
ORIGIN      
        1 ttacccgccg acggtgaaca cctactggcg acgtcgtggc agcacatatt ttgtatcaaa
       61 agccggtgag cgttatcgcc gggatgtggc acttattgtt cgccagcagc ggctgaaatt
      121 aaacctgtcc ggaaggctgg tgataaagat tattgcagag ccaccggata agcgccgtcg
      181 tgacctggac aatatcctga aagcaccact ggatgtgctg acgcatgccg gacttctcat
      241 agacgacgag cagtttgatg aaatcaatat tgtgcgcggt cagctcgttc ctggtgagcg
      301 gctggggata aaaatcacag aactggagtg cgcatgaata accactattt acagtttgtg
      361 cgtgagcagc tcattatcgc caccgc
//
LOCUS       DAZKIF010000505          369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZKIF010000505 DAZKIF010000000
VERSION     DAZKIF010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.510"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>369
                     /locus_tag="ACHOTW_005313"
     CDS             <1..>369
                     /locus_tag="ACHOTW_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9847191.1"
                     /translation="NHAAQFGALVDKFRADLADMAGQCVGGSADGVPWICGDTTYFWK
                     QKNESSYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKWR
                     DSSATWTSQDRASHFSAWARR"
BASE COUNT           98 a           88 c          112 g           71 t
ORIGIN      
        1 aaatcacgca gcacagtttg gtgcgctggt tgataaattc cgtgcagacc tggcggatat
       61 ggcaggtcag tgcgtcggtg gctctgctga cggtgttccc tggatatgcg gggacacgac
      121 gtatttctgg aagcagaaga acgaatcctc gtaccagacg gtgtacggca gctacaaaaa
      181 caaaacggaa aagaatatcc atttcgtacc gttcatgacg gatgagaatg gggtgaatgt
      241 gccgacgaac aaaccggaag aagacccgga cattccgggt atcggatatt acggttcgaa
      301 atggcgtgac agctcagcca cctggacgtc acaggacagg gcgagccatt tcagcgcctg
      361 ggcacgccg
//
LOCUS       DAZKIF010000506          369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZKIF010000506 DAZKIF010000000
VERSION     DAZKIF010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.511"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>369
                     /locus_tag="ACHOTW_005314"
     CDS             <1..>369
                     /locus_tag="ACHOTW_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847192.1"
                     /translation="NHAAQFGALVDKFRADLADMAGQCVGGSAGGVPWICGDTTYFWK
                     QKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKWR
                     DSSATWTSQDRASHFSSWARR"
BASE COUNT           97 a           87 c          112 g           73 t
ORIGIN      
        1 aaatcacgcc gcacagtttg gtgcgctggt tgataaattc cgtgcagacc tggcggatat
       61 ggcaggccag tgcgtcggtg gctctgctgg cggtgttccc tggatatgcg gggacacgac
      121 gtatttctgg aagcagaaga acgaatccac gtaccagacg gtgtatggca gctataaaaa
      181 caaaacggaa aagaatatcc atttcgtacc gttcatgacg gatgagaacg gggtgaatgt
      241 gccgacgaac aaaccggaag aagacccgga cattccgggt atcggatatt acggttcgaa
      301 atggcgtgac agctcagcca cctggacgtc acaggacagg gcgagccatt tcagttcatg
      361 ggctcgccg
//
LOCUS       DAZKIF010000507          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZKIF010000507 DAZKIF010000000
VERSION     DAZKIF010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.512"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>368
                     /locus_tag="ACHOTW_005315"
     CDS             <1..>368
                     /locus_tag="ACHOTW_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFP9847193.1"
                     /translation="QIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALD
                     EDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQ
                     PQEHVYWLHKIGNLTLISGS"
BASE COUNT          131 a           60 c           69 g          108 t
ORIGIN      
        1 aacaaattaa aagccaaaga gaaatggttt gttattcagc tctggtagca ataaataatg
       61 atatgccttt tgattctgtg ataaatcaga taaaccaaca tgcagataat tcaggtttta
      121 tagctgctct ggatgaagat ttatatgagc ctcgtccgaa tcaagtgaac ttaataaaag
      181 caattttgct tcgcttagat atggagcaac aagatgagag cgtgattaaa acctatacgg
      241 ggagaatcac aattgagcat attcttcctc aagcattagt taatgaatac tggattaata
      301 gattccaacc tcaagagcat gtctactggc tgcataaaat aggtaattta acactcataa
      361 gtggttcc
//
LOCUS       DAZKIF010000508          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZKIF010000508 DAZKIF010000000
VERSION     DAZKIF010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.513"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>368
                     /locus_tag="ACHOTW_005316"
     CDS             <1..>368
                     /locus_tag="ACHOTW_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9847194.1"
                     /translation="DWLKKEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMA
                     VMGLAGVLRGKKVRTTVSRKAVAACDRVNRQFVAERPDQLWVADFTWVSTWQGFVYVA
                     FIIDVFAGYIVGWQVSSSMET"
BASE COUNT           84 a           82 c          115 g           87 t
ORIGIN      
        1 gactggctga agaaagagat acagcgcgta tacgatgaaa atcaccaggt atacggtgtg
       61 cgtaaagtct ggcgtcagtt gttacgggaa ggtatcagag tggccagatg cactgtggca
      121 cgtctcatgg cagttatggg acttgccggt gttctccggg gtaaaaaggt ccgcactacc
      181 gtcagccgga aagccgttgc cgcatgcgac cgcgtaaacc gtcagttcgt ggcagaacgt
      241 ccagaccagt tgtgggtggc tgattttacc tgggtaagca catggcaggg cttcgtttat
      301 gtggcgttca tcattgatgt gttcgccgga tacatcgtgg gatggcaggt ctcatcatcc
      361 atggaaac
//
LOCUS       DAZKIF010000509          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZKIF010000509 DAZKIF010000000
VERSION     DAZKIF010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.514"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>368)
                     /locus_tag="ACHOTW_005317"
     CDS             complement(<1..>368)
                     /locus_tag="ACHOTW_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9847195.1"
                     /translation="QLATKYQTVIKLYHIDSEPLKALPQSNIWPVSIYYRLLSFDYFS
                     ARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSY
                     FNSGVMYINLREWLKQRLTE"
BASE COUNT          128 a           76 c           47 g          117 t
ORIGIN      
        1 ttttccgtta gtctttgttt taaccattcc cgtaaattaa tatacattac accagagtta
       61 aaatagctac cgttaaaatc ctcattacac aaacgctcag cgcttttact ttgcatagca
      121 tctacatcaa ttaccactgc cccatattca tctttaaact ctaatgctat taactcgttc
      181 aatgaaccct tacagacgat atcagcatca agatataata atgaatccaa tcgcgcagaa
      241 aaataatcaa atgaaagcaa acgataataa atagatactg gccagatatt tgattgaggt
      301 agcgccttca atggctcaga atcaatatga tataatttaa ttactgtttg atacttcgtt
      361 gctaattg
//
LOCUS       DAZKIF010000510          365 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZKIF010000510 DAZKIF010000000
VERSION     DAZKIF010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.515"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(194..>365)
                     /locus_tag="ACHOTW_005318"
     CDS             complement(194..>365)
                     /locus_tag="ACHOTW_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HFP9847196.1"
                     /translation="VTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPEPLG
                     GGGARMACGIIQ"
BASE COUNT           96 a           86 c           98 g           85 t
ORIGIN      
        1 taaaagcgaa aaaatcccgc agtgctcacg gacaggaact gcgggagcgt tacgaagatt
       61 gagtgtaagg aattattctt atgtcacgac aaaaacatta actcagagag ggaggatgtg
      121 ccgttctttt cagggagaaa ggatttatcg tcctgaggaa taaaggtaag gggcccgccc
      181 cttacctgac tgattattga atgatgccgc aggccattct cgcaccacca ccgcccaggg
      241 gctccggatg gtcatgatgg ttatcaccgc cagcatgaag catgagagaa cgccctttaa
      301 tctcttttaa tgagttcagt ctcggggcca ggaccgggta gttcgctttt ccgtcatgcg
      361 tcacg
//
LOCUS       DAZKIF010000511          358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZKIF010000511 DAZKIF010000000
VERSION     DAZKIF010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.516"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..>358
                     /locus_tag="ACHOTW_005319"
     CDS             2..>358
                     /locus_tag="ACHOTW_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP9847197.1"
                     /translation="MMTNINTACVKNNASYQFNNALPNKETISSNFCERLEQWGNKSL
                     NNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFTD
                     NASLVNINLSFNKINTI"
BASE COUNT          126 a           66 c           59 g          107 t
ORIGIN      
        1 gattatgacc aatattaata cagcttgtgt aaaaaataat gccagttatc aatttaataa
       61 cgcattacct aacaaagaaa ccatctccag caatttttgt gaacgactag aacaatgggg
      121 taataagtcg cttaataatg gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc
      181 ttacaattcg aacatggcat ctcttgattt gtcatatctt gatttgagtg agttaccgcc
      241 tatcccctcg acagttaata cgttaaatct ggaaaacaat tgtctaactt gtcttgactt
      301 tactgacaat gccagtctcg ttaatatcaa cctcagcttt aataaaatta acacgata
//
LOCUS       DAZKIF010000512          353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZKIF010000512 DAZKIF010000000
VERSION     DAZKIF010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.517"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>353
                     /locus_tag="ACHOTW_005320"
     CDS             <1..>353
                     /locus_tag="ACHOTW_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9847198.1"
                     /translation="YKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISDSFGGPL
                     SLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAAGGGVAV
                     LAALAAAAWQAEQAD"
BASE COUNT           66 a           97 c          116 g           74 t
ORIGIN      
        1 gtataaggct gccatgcgca cactgcccgc acagttcacg gatattgtca ctcagcttgc
       61 cggtggtcag aatcccttcc ttatcatgct gcagcagggg gggcagatca gcgattcatt
      121 cggtggaccg ctcagcctgc ttaccctgct gaaggaggaa cttctcggga tcagggatgc
      181 ctctgaatca tcagaggagt cgctgtcaga tacggcaaat gcactggctg aaaatgcccg
      241 gaatgccggt gagctgggac gatttatgtc ggtggcccgt gtggcggcag gtggcggggt
      301 tgccgtactg gccgcgcttg ctgccgccgc ctggcaggca gagcaggctg acc
//
LOCUS       DAZKIF010000513          348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZKIF010000513 DAZKIF010000000
VERSION     DAZKIF010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.518"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>348
                     /locus_tag="ACHOTW_005321"
     CDS             <1..>348
                     /locus_tag="ACHOTW_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9847199.1"
                     /translation="KGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLAL
                     EAELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETL
                     EYKRQLAELGDKVE"
BASE COUNT          104 a           79 c          116 g           49 t
ORIGIN      
        1 aaaggtccgg ggcagcagga agccgtcagc cgtctggctg aagagctgac aggtaaaaag
       61 catacatcac cgaaaacgcg ctctgccggg gagagggaag aggagcaggc aagagaggct
      121 ctgcttgccc ttgaagctga gctcaggacg ctggaaaaac acagcggtgc gaatgagaaa
      181 atcagccggc agcgccgtga tttatggaag gcggaaagtc agtatgcggt cctgaaagag
      241 gctgccacga aacgacagtt atctgagcag gaaaaatccc tgctggcgca taaagacgag
      301 acgctggagt acaaacgcca gctggctgag ctgggcgaca aggttgaa
//
LOCUS       DAZKIF010000514          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZKIF010000514 DAZKIF010000000
VERSION     DAZKIF010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.519"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            104..>344
                     /locus_tag="ACHOTW_005322"
     CDS             104..>344
                     /locus_tag="ACHOTW_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9847200.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPP"
BASE COUNT           67 a           76 c          128 g           73 t
ORIGIN      
        1 aacggacaga tagggccgga ggcggcaggc agtcttgtgc agctgattga ccagcgggtg
       61 caggcggtga tgctgtccat gcgacgtgac ggaggaatgc tgagtggctg agataaaaac
      121 gctgcatctg gtcccgcgtg aagggatgca ggtgagtgag aagccgtcgg tggtgagggt
      181 gcggtttggt gacggttatg aacagcgccg ccccacaggg ctgaatcctc aactgaagac
      241 gtttcaggcg gtgttccggg tgacggatga gtcaacccgg cgctggctgg atgaattttt
      301 atcctggcat ggtggttacc gtgccttttt gtggcgaccg ccga
//
LOCUS       DAZKIF010000515          334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZKIF010000515 DAZKIF010000000
VERSION     DAZKIF010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.520"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(43..315)
                     /locus_tag="ACHOTW_005323"
     CDS             complement(43..315)
                     /locus_tag="ACHOTW_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9847201.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
BASE COUNT           65 a           94 c           87 g           88 t
ORIGIN      
        1 gaaaaaggcc agcagctgtt tactgatggc cctgactccc cgttacagca tcatgaccga
       61 ttcgggttga ggttcagtcg catcggcgac cggtgattca ggctgaactt caccgctctc
      121 tgcggtggta tctcccgctt cagtcggtgg ctctgcctgt acaccaagca gttcatccag
      181 aatggcatcc acttctgcat caagacgcgc ttccaggtta tggcgaagtt tctgtttcag
      241 tgcgctccgg acttcttcag agcgcaggac gtccttcact gcctctgcag tgaccaggga
      301 tgtaatttct gacatgggat tttctcgtcg aaag
//
LOCUS       DAZKIF010000516          333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZKIF010000516 DAZKIF010000000
VERSION     DAZKIF010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.521"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          107 a           69 c           64 g           93 t
ORIGIN      
        1 agatgttatg cagtacgcac aaaaaaacag actggatttg cttgctgatc tataatcttt
       61 atctaaatta ctattggcgc tttggataat aacataagca tatattacct cctagcaata
      121 atagatataa cctcatatca aggtgtcact ggtaaataat cggataaaag acagtaccgg
      181 tcggcacata gagcttcttc tatttcacca gaagtgcaca gcagggaact accaatcaca
      241 cggttagtgt catgggtgga ggatgcgtcc cgttctttcc ctataacggg gatacgcagt
      301 ttttatcagt acatgcagaa aaacccctaa ttc
//
LOCUS       DAZKIF010000517          333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZKIF010000517 DAZKIF010000000
VERSION     DAZKIF010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.522"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>333
                     /locus_tag="ACHOTW_005324"
     CDS             <1..>333
                     /locus_tag="ACHOTW_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9847202.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAK"
BASE COUNT           74 a           85 c          103 g           71 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaa
//
LOCUS       DAZKIF010000518          330 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZKIF010000518 DAZKIF010000000
VERSION     DAZKIF010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.523"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>330)
                     /gene="espR4"
                     /locus_tag="ACHOTW_005325"
     CDS             complement(<1..>330)
                     /gene="espR4"
                     /locus_tag="ACHOTW_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HFP9847203.1"
                     /translation="QGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASS
                     VKEGGSNDLKENVDRALVRMFKCVKLHSNELDLSYLFLGSVPPLPDWIEMLSLVYNEL
                     DSIQVPES"
BASE COUNT           99 a           77 c           56 g           98 t
ORIGIN      
        1 gattcgggaa cctgtattga atcaagttca ttataaacaa ggctaagcat ttcaatccaa
       61 tctggaagag gcggcacaga acccaaaaat aggtatgata agtcaagttc attactgtga
      121 agcttaacac atttaaacat tctcaccaac gctctatcta cattttcttt gaggtcattc
      181 gatccgcctt ctttgactga actagcccag tcactgaagc ctttgcaaag tctggcatct
      241 gtaattttcg ttaacttgtc cagcaactct gcaatattag tcgatataaa ctctaattta
      301 tgccgaatgg gtaaagtccc ctccccctga
//
LOCUS       DAZKIF010000519          328 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZKIF010000519 DAZKIF010000000
VERSION     DAZKIF010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.524"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>328
                     /locus_tag="ACHOTW_005326"
     CDS             <1..>328
                     /locus_tag="ACHOTW_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9847204.1"
                     /translation="ATAILRHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYL
                     ASESEGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALL
                     QKGGQIR"
BASE COUNT           78 a           80 c          120 g           50 t
ORIGIN      
        1 gcaacggcga ttttgcgcca tgcgggaaga gtggcgctaa acgcgggggc atcatcgaca
       61 gtatcagagg tgcgcccgtc atcgccttcc ggtgcagaag ccacaggcgt cacaacactg
      121 ctctcttacc ttgccagcga gtcagaggga agcctgaaag tacagggatg gtcagccagt
      181 ggcggcaggg cagaagtggt cagcgatgcg gagggaaccg gaggtaaggc agtgaagctg
      241 accaaggaag ccggtaaaag cagctgggtg ctggagtacg ccgcgggcaa cggtgcggct
      301 ctgttacaga aaggggggca gattcgct
//
LOCUS       DAZKIF010000520          326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZKIF010000520 DAZKIF010000000
VERSION     DAZKIF010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.525"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            11..>326
                     /gene="rfbE"
                     /locus_tag="ACHOTW_005327"
                     /gene_synonym="perA"
     CDS             11..>326
                     /gene="rfbE"
                     /locus_tag="ACHOTW_005327"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFP9847205.1"
                     /translation="MKYIPVYQPSLTGKEKEYVNECLDSTWISSKGNYIQKFENKFAE
                     QNHVQYATTVSNGTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFVDS
                     DNE"
BASE COUNT          123 a           50 c           63 g           90 t
ORIGIN      
        1 gaggatgaaa atgaaatata taccagttta ccaaccgtca ttgacaggaa aagaaaaaga
       61 atatgtaaat gaatgtctgg actcaacgtg gatttcatca aaaggaaact atattcagaa
      121 gtttgaaaat aaatttgcgg aacaaaacca tgtgcaatat gcaactactg taagtaatgg
      181 aacggttgct cttcatttag ctttgttagc gttaggtata tcggaaggag atgaagttat
      241 tgttccaaca ctgacatata tagcatcagt taatgctata aaatacacag gagccacccc
      301 cattttcgtt gattcagata atgaaa
//
LOCUS       DAZKIF010000521          325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZKIF010000521 DAZKIF010000000
VERSION     DAZKIF010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.526"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>325
                     /locus_tag="ACHOTW_005328"
     CDS             <1..>325
                     /locus_tag="ACHOTW_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFP9847206.1"
                     /translation="ADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKAL
                     FMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWG
                     TLKNE"
BASE COUNT           87 a           77 c           93 g           68 t
ORIGIN      
        1 tggcggacct gacgtatgtt gccacacagg agggatggtt gtacctcgct ggcatcaaag
       61 atgtttatac gtgcgaaatt gtcggctacg ccatgggaga gcgcatgaca aaagagctga
      121 caggtaaagc cctgtttatg gcgctcagga gccagcgccc acctgccggg ctaatccacc
      181 actctgatcg aggttcacag tactgcgcat acgattaccg ggtcatacag gagcagtttg
      241 gtctgaaaac atcaatgtcg cgtaaaggta actgttacga caacgctccg atggaaagct
      301 tctggggaac gctgaaaaat gagag
//
LOCUS       DAZKIF010000522          314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZKIF010000522 DAZKIF010000000
VERSION     DAZKIF010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.527"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>314
                     /locus_tag="ACHOTW_005329"
     CDS             <1..>314
                     /locus_tag="ACHOTW_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9847207.1"
                     /translation="LMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGVYT
                     SANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSA
                     GLV"
BASE COUNT           63 a           77 c          114 g           60 t
ORIGIN      
        1 cttatgaatg ccgcgattgt caacggaatt aagacggcgg caaacggtat gtccggtgcg
       61 ggaggatttc tcggcagcat tggtgactgg ctgggcggag cggtggccaa tgcaaaaggc
      121 ggcgtgtata cctcggcaaa cctgagcgcg tacagcaaca gcattgtgga cacgcccacg
      181 tactttgcgt ttgcaaaagg ggccgggctg atgggggaag ccggacctga agctattatg
      241 cccctgaccc gggcggcgga tggctcgctg ggcgtacgcg ccgtgggcag tatgaacggc
      301 agtgctggtc tggt
//
LOCUS       DAZKIF010000523          308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZKIF010000523 DAZKIF010000000
VERSION     DAZKIF010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.528"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(44..289)
                     /locus_tag="ACHOTW_005330"
     CDS             complement(44..289)
                     /locus_tag="ACHOTW_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9847208.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
BASE COUNT           63 a           96 c           76 g           73 t
ORIGIN      
        1 taaaaaagac caccggcaca caccgatggt ccctgacgca tgcttacatc atcatgtcgc
       61 tgtccggtgt ggggtcaccg ctatctgaag cactcccctc acccgcgatg ccttccggct
      121 ccggagctgc cggtgcgccc agcagctcat ccagaatggc atcaacctct gcatcaagac
      181 gcgcttccag gttatggcga agtttctgtt tcagtgcgct ccggacttct tcagagcgca
      241 ggacttcctt cactgcttca gcagtgacca gggatgtaat ttctgacatg ggattttctc
      301 gtcgaaag
//
LOCUS       DAZKIF010000524          305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZKIF010000524 DAZKIF010000000
VERSION     DAZKIF010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.529"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>305
                     /locus_tag="ACHOTW_005331"
     CDS             <1..>305
                     /locus_tag="ACHOTW_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9847209.1"
                     /translation="AGASMAAWGAALSAGGFSATTMLFSLGASMILGGVAQMLAPKPK
                     TPEYRATDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGKV
                     "
BASE COUNT           68 a           79 c           99 g           59 t
ORIGIN      
        1 gccggagcat cgatggcagc ctggggtgca gcgctgagtg ccggcggttt ttctgccacc
       61 acgatgctgt tttcactggg tgccagcatg atactgggtg gtgtggcaca gatgctggcc
      121 ccgaagccaa aaacaccgga atacagggca acggataacg gtaaacagaa cacgtacttt
      181 tcgtcgctgg acaacatgat tgcccagggt aacccgatgc cggtgcctta cggggaaatg
      241 ctggttggct cccgccgtat atcccaggac atcagtaccc gtgatgaagg cgggggcgga
      301 aaggt
//
LOCUS       DAZKIF010000525          302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZKIF010000525 DAZKIF010000000
VERSION     DAZKIF010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.530"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>302
                     /gene="cesT"
                     /locus_tag="ACHOTW_005332"
     CDS             <1..>302
                     /gene="cesT"
                     /locus_tag="ACHOTW_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFP9847210.1"
                     /translation="EYMMIYGVCGKFPTDNSNFALEILNANLWFAENGGPYLCYEAGA
                     QSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISS"
BASE COUNT           97 a           48 c           69 g           88 t
ORIGIN      
        1 gaatatatga tgatttatgg tgtctgtggg aaattcccga cagataactc taacttcgct
       61 cttgagattt tgaatgcaaa cttatggttt gcagagaatg gtgggccata tctgtgctat
      121 gaggctggag cacaatcgct gttgttagcg ttacgtttcc ctctcgatga tgctacccct
      181 gaaaaactcg agaatgaaat agaagtcgtt gttaagtcaa tggaaaacct gtatttggta
      241 ttacataatc agggaataac attagaaaac gaacatatga aaatagagga aatcagttca
      301 ag
//
LOCUS       DAZKIF010000526          301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZKIF010000526 DAZKIF010000000
VERSION     DAZKIF010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.531"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..189
                     /locus_tag="ACHOTW_005333"
     CDS             <1..189
                     /locus_tag="ACHOTW_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9847211.1"
                     /translation="GVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLFGLLTYL
                     TNLYFKIREDRRKTARGD"
     gene            194..>301
                     /locus_tag="ACHOTW_005334"
     CDS             194..>301
                     /locus_tag="ACHOTW_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFP9847212.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLP"
BASE COUNT           77 a           60 c           90 g           74 t
ORIGIN      
        1 ggtgtgtcat acaccacgtc agcggtaggg acgggatact ggttactgca actgctggac
       61 aaagtctctc cgtcccagtg ggtggcaata ggtgtactgg ggagtctgct gtttggcctg
      121 ctgacgtatc tgactaacct gtatttcaaa atcagggagg accgccgtaa gacggcgcgg
      181 ggagactaaa gcgatgaaga aaaaatacga actggttgtt aaagagataa ataattaccc
      241 ggataagatt gctgttactg tggcacttga aattggcggg catccgtcgt tgttgttgcc
      301 a
//
LOCUS       DAZKIF010000527          300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZKIF010000527 DAZKIF010000000
VERSION     DAZKIF010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.532"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..>300
                     /locus_tag="ACHOTW_005335"
     CDS             16..>300
                     /locus_tag="ACHOTW_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9847213.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEH"
BASE COUNT           88 a           80 c           73 g           59 t
ORIGIN      
        1 gccacctgat taaaggtgat atcctcacca caacacaaaa caggtgactt aatgaacaag
       61 aaaaccaaac gtactttcac ccctgaattc aggctggaat gtgcacagct aattgttgat
      121 aagggctact catatcgaca agccagtgaa gcgatgaatg tcggttctac cacgcttgag
      181 agttgggtgc gccagctcag gcgagagcgt caggggattg cgccctctgc cacacctatt
      241 actccagacc agcaacgtat ccgcgaactg gaaaagcagg ttcgccgcct ggaggaacac
//
LOCUS       DAZKIF010000528          299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZKIF010000528 DAZKIF010000000
VERSION     DAZKIF010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.533"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>299
                     /locus_tag="ACHOTW_005336"
     CDS             <1..>299
                     /locus_tag="ACHOTW_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9847214.1"
                     /translation="TTTAELWKMAGVISDEAGGGIRQAAENLARLAESGKYTAGQLRI
                     MGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYNFLSVSQLRHIDEL"
BASE COUNT           77 a           67 c          101 g           54 t
ORIGIN      
        1 caccacaacg gcagaattgt ggaaaatggc cggggtgatc agcgatgaag ccggtggtgg
       61 tatcagacag gcggcagaaa atctggcccg tctggcagaa agcgggaaat ataccgccgg
      121 gcagctacgg atcatggggg aaacctctca gagatggctg cagacggtgg gggacgatgc
      181 cgggaaggtg gaaaaagcct ttgaagggat tgcagcagat ccggtgaagg cgctggcctc
      241 cctgaatcag cagtataact tcctgagcgt ttcccagtta cgccatattg atgagcttg
//
LOCUS       DAZKIF010000529          295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZKIF010000529 DAZKIF010000000
VERSION     DAZKIF010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.534"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..204
                     /locus_tag="ACHOTW_005337"
     CDS             <1..204
                     /locus_tag="ACHOTW_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9847215.1"
                     /translation="FRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDIT
                     RGATYPVLIDSIAVEVNSTDTAA"
BASE COUNT           80 a           68 c           85 g           62 t
ORIGIN      
        1 ttccgctttg ccgggaataa cagcctgcag gtgacgccgg ttattgatgg tcaggatggc
       61 acaccgttca cgctgacgca gtcaccggtc agtgcctttg cggcggataa actgcatgtg
      121 acagacatta ccagaggtgc gacttacccg gtactgatag acagcattgc ggtggaagtg
      181 aacagcacag acactgcggc atgataaaaa aaccgccagc gacaggaatg gacgctggcg
      241 gtggtgatac ctatggagaa aaaataaagg aacgatactt tcgtactctg gtttt
//
LOCUS       DAZKIF010000530          290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZKIF010000530 DAZKIF010000000
VERSION     DAZKIF010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.535"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>290)
                     /locus_tag="ACHOTW_005338"
     CDS             complement(<1..>290)
                     /locus_tag="ACHOTW_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9847216.1"
                     /translation="VGWRSLFQAVAEPFAGAWQQGVKADPETVLSFHAVFSCISLISQ
                     DIAKMRLRLMQTDVQGIRREKRQGDTARLCRRPNAQQNRIQFFELWLNSKLR"
BASE COUNT           75 a           87 c           72 g           56 t
ORIGIN      
        1 tgacgcaatt tggaattcag ccacagttca aaaaactgga tgcgattctg ctgcgcatta
       61 ggacgacgac agagacgggc agtatctccc tgccgctttt cccggcgtat tccctgtaca
      121 tcagtctgca tcaggcgaag ccgcattttg gcaatatcct gggatatcag cgaaatgcat
      181 gaaaacaccg cgtgaaagga caaaacggtt tccggatcgg ctttcacccc ctgctgccag
      241 gcaccggcaa aaggctcagc caccgcctga aacagggacc gccagcccac
//
LOCUS       DAZKIF010000531          290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZKIF010000531 DAZKIF010000000
VERSION     DAZKIF010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.536"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>290)
                     /locus_tag="ACHOTW_005339"
     CDS             complement(<1..>290)
                     /locus_tag="ACHOTW_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9847217.1"
                     /translation="AGWTSLFQAVAEPFSGAWQQGVKADPEAVLSFHAVFACISLISQ
                     DIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQNRIQFFELWLNAKLR"
BASE COUNT           65 a           87 c           85 g           53 t
ORIGIN      
        1 tgacgcagtt tggcgttcag ccacagttca aaaaactgga tgcggttctg ctgggcgttg
       61 ggacgacgac agaggcgggc aatatccccc cggcgcgttt ccctgcgtat cccatgcgca
      121 tccgtctgca taagacgcag ccgcattttg gcgatatcct gggatatcag cgaaatacat
      181 gcaaacaccg catgaaagga gaggacggct tcaggatcgg ctttcacgcc ctgctgccag
      241 gcgccggaaa agggctcagc caccgcctga aacaggctgg tccagcccgc
//
LOCUS       DAZKIF010000532          282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZKIF010000532 DAZKIF010000000
VERSION     DAZKIF010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.538"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..>282
                     /locus_tag="ACHOTW_005340"
     CDS             2..>282
                     /locus_tag="ACHOTW_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847218.1"
                     /translation="MTWDDEQWEVFVHDWLIVCKSDDYPWSERLGGAGDKGRDVVGYK
                     SDPNVEGYSWDNYQCKLYKKSLGFSDVVVEFGKLIYFTLNGDYPIPQKY"
BASE COUNT           83 a           37 c           71 g           91 t
ORIGIN      
        1 attgacatgg gatgatgaac aatgggaggt atttgtccat gattggctta ttgtctgtaa
       61 atcagatgat tacccgtgga gcgaacgttt gggaggagct ggagataaag gtagagacgt
      121 tgttggatat aaatcggatc ctaacgtaga aggttattct tgggataatt atcaatgcaa
      181 actgtacaaa aaaagtttag ggttctctga tgttgtagtt gagtttggaa aacttatcta
      241 ttttactctg aatggtgatt atcccatccc tcagaagtac tt
//
LOCUS       DAZKIF010000533          278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZKIF010000533 DAZKIF010000000
VERSION     DAZKIF010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.539"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            24..100
                     /locus_tag="ACHOTW_005341"
     tRNA            24..100
                     /locus_tag="ACHOTW_005341"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHOTW_005342"
     tRNA            143..218
                     /locus_tag="ACHOTW_005342"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZKIF010000534          267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZKIF010000534 DAZKIF010000000
VERSION     DAZKIF010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.540"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>267)
                     /locus_tag="ACHOTW_005343"
     CDS             complement(<1..>267)
                     /locus_tag="ACHOTW_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFP9847219.1"
                     /translation="KYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPV
                     SRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGI"
BASE COUNT           77 a           51 c           45 g           94 t
ORIGIN      
        1 aaaatgcctg ccatataatg cttttgttta aatttatgtt ttgcacaata ccgatactga
       61 ttgctattta caggtttaag taaatcttct ttactccacg gagaagctgc tttggcgatc
      121 aaaaaactac gtgacaccgg atagtcagcc cactcatgcc aaggttttgt aggtcccaca
      181 tagtgtataa acacggtgtc atcattgact ggattatcaa ctttgtcttt taattcatag
      241 ttaatactat atcgggtatt atatttc
//
LOCUS       DAZKIF010000535          262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZKIF010000535 DAZKIF010000000
VERSION     DAZKIF010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.541"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>262)
                     /locus_tag="ACHOTW_005344"
     CDS             complement(<1..>262)
                     /locus_tag="ACHOTW_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HFP9847220.1"
                     /translation="FSAFQNGHADAIKAYCGVLGNSNLKRGEIIRMLEARNYDGAPGL
                     LLAYQNGDINTIQSFFDSLIMLDISKDFIEELLTAKHYDFTGL"
BASE COUNT           87 a           57 c           33 g           85 t
ORIGIN      
        1 ataaccccgt aaaatcataa tgttttgcag tcaataactc ttcgataaaa tcctttgata
       61 tatctaacat tattagtgag tcaaaaaaag actgaatcgt attaatatct ccattttgat
      121 acgcaagaag taatcctggt gcaccatcat aatttctcgc ctcaagcatc ctgattatct
      181 cacctctttt caaattacta ttgccaagta cgccacaata tgcctttatt gcgtctgcat
      241 gaccattttg aaatgcagaa aa
//
LOCUS       DAZKIF010000536          260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZKIF010000536 DAZKIF010000000
VERSION     DAZKIF010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.542"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>260)
                     /locus_tag="ACHOTW_005345"
     CDS             complement(<1..>260)
                     /locus_tag="ACHOTW_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847221.1"
                     /translation="QNTDCHDESRRVTHMSRQITTDTDTDKELNPTHNARMRESATTG
                     ELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA"
BASE COUNT           44 a           70 c           69 g           77 t
ORIGIN      
        1 tgctgcccgc tgtcgaaaat ccggcgacgg ttgccagact ccagtcatcg gaaatttccc
       61 gatcggttcg ctcaggccgt ccaggtattc aggttcggct gtctgcaacg acgcgtcatt
      121 caactcaccg gtcgtagcac tctcgcgcat gcgcgcgtta tgtgtggggt ttaattcttt
      181 atctgtatct gtatctgtcg tgatttgtcg tgacatatgc gtgacgcgtc gtgactcatc
      241 gtgacaatca gtgttctgct
//
LOCUS       DAZKIF010000537          259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZKIF010000537 DAZKIF010000000
VERSION     DAZKIF010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.543"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>259
                     /locus_tag="ACHOTW_005346"
     CDS             <1..>259
                     /locus_tag="ACHOTW_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9847222.1"
                     /translation="IRIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRHD
                     EYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFYTRIQ"
BASE COUNT           60 a           88 c           80 g           31 t
ORIGIN      
        1 tatccgcatc ccgtatgcga ccgacccggc gggaaaccgg ctgccggacc cggagctaca
       61 cccggacagc acgctcacgg cgtggccgga taaccgcatc gcggaggatg cgcactatgt
      121 ctaccgacac gatgaatacg gcaggctgac ggagaagacg gaccgcatcc cggcgggtgt
      181 gatacggacg gacgacgagc ggacccacca ctaccactac gacagccagc accgcctggt
      241 gttctacacg cggatacag
//
LOCUS       DAZKIF010000538          256 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZKIF010000538 DAZKIF010000000
VERSION     DAZKIF010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.544"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..256)
                     /locus_tag="ACHOTW_005347"
                     /pseudo
     CDS             complement(1..256)
                     /locus_tag="ACHOTW_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer"
BASE COUNT           45 a           72 c           68 g           71 t
ORIGIN      
        1 tgcagcccgc tgtcgaaaat ccagcgacgg ccgccagaca ccagtcatcg gaaatttccc
       61 gatcggttcg ctcaggccat ccgggtattc aggttctgct gtctgcaacg gcgcgccatt
      121 cgactcactg gctggagcac tctcgcgcat gcgcgcgtta tgtgtggggt ttaattcttt
      181 atctgtatct gtatctgtat ctgtcgtgac atatgcgtga cgcgtcgtga ctcatcgtga
      241 caatcagtgt tctgct
//
LOCUS       DAZKIF010000539          253 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZKIF010000539 DAZKIF010000000
VERSION     DAZKIF010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.545"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT           92 a           42 c           45 g           74 t
ORIGIN      
        1 acattaaaga aaaaacgtaa aatcaaatac ataaaataag tcaccaaggg atcattttca
       61 gaaaacaacg tcaacgtaac gtcaactctg taagaacttt tttggtcact ttttgggttg
      121 aatgtacgta aaaatcccca gcagtgaaat ttgagcgcgt aagcacaatt ctgaggggct
      181 ttttgtctgt aggttaggca gtgtaactaa caatacaaga agttagctct gactaactat
      241 ttaattaact ttt
//
LOCUS       DAZKIF010000540          248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZKIF010000540 DAZKIF010000000
VERSION     DAZKIF010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.546"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            126..>248
                     /locus_tag="ACHOTW_005348"
     rRNA            126..>248
                     /locus_tag="ACHOTW_005348"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           78 a           45 c           63 g           62 t
ORIGIN      
        1 agacaatctg tgtgggcact cgaagatacg gattcttaac gtcgcaagac gaaaaatgaa
       61 taccaagtct caagagtgaa cacgtaattc attacgaagt ttaattcttt gagcatcaaa
      121 cttttaaatt gaagagtttg atcatggctc agattgaacg ctggcggcag gcctaacaca
      181 tgcaagtcga acggtaacag gaagaagctt gcttctttgc tgacgagtgg cggacgggtg
      241 agtaatgt
//
LOCUS       DAZKIF010000541          245 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZKIF010000541 DAZKIF010000000
VERSION     DAZKIF010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.547"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>245)
                     /locus_tag="ACHOTW_005349"
     CDS             complement(<1..>245)
                     /locus_tag="ACHOTW_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFP9847223.1"
                     /translation="KMPEDSIFTKINTRIPRDTMPILRRYHLWPDGWNIRGLNGFMLS
                     HKGSEVIDAVIAGQNQAYRELRRIRDNIHSEIYFKQT"
BASE COUNT           66 a           51 c           43 g           85 t
ORIGIN      
        1 cagtttgttt gaagtatatt tcactatgaa tattatctct tattcttctt agttccctgt
       61 aagcctgatt ctggcctgcg ataacagcat caatcacttc actaccttta tgtgatagca
      121 tgaatccatt gagcccacga atattccatc catcaggcca taggtgataa cgacgaagga
      181 tgggcattgt gtctcgagga atccttgtat tgatcttagt aaaaatacta tcttctggca
      241 tttta
//
LOCUS       DAZKIF010000542          229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZKIF010000542 DAZKIF010000000
VERSION     DAZKIF010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.denovo.549"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>229
                     /locus_tag="ACHOTW_005350"
     CDS             <1..>229
                     /locus_tag="ACHOTW_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9847224.1"
                     /translation="CRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQDLISRTKA
                     ALQKNPKNVLLAVCWMQGEFDMSAATHAQQPA"
BASE COUNT           49 a           62 c           75 g           43 t
ORIGIN      
        1 ctgtcgtggt ggttcggcat ttacccaggg cgcggagggg acattcagcg agtccacggg
       61 ggccagtcag gattcggcac gctggggggt gggcaagccg ttatatcagg atctgatttc
      121 ccgcacaaaa gcggcattgc agaaaaatcc caaaaacgtt ctgctggccg tctgctggat
      181 gcagggtgag tttgacatga gcgccgccac ccacgcacag caacctgcg
//
LOCUS       DAZKIF010000543        16213 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.guided.105, whole genome shotgun sequence.
ACCESSION   DAZKIF010000543 DAZKIF010000000
VERSION     DAZKIF010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.guided.105"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            45..1178
                     /locus_tag="ACHOTW_005351"
     CDS             45..1178
                     /locus_tag="ACHOTW_005351"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9847225.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1266..1799
                     /gene="blc"
                     /locus_tag="ACHOTW_005352"
     CDS             1266..1799
                     /gene="blc"
                     /locus_tag="ACHOTW_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9847226.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1796..2113)
                     /gene="sugE"
                     /locus_tag="ACHOTW_005353"
     CDS             complement(1796..2113)
                     /gene="sugE"
                     /locus_tag="ACHOTW_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9847227.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2289..2435)
                     /gene="ecnB"
                     /locus_tag="ACHOTW_005354"
     CDS             complement(2289..2435)
                     /gene="ecnB"
                     /locus_tag="ACHOTW_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9847228.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2546..2671)
                     /gene="ecnA"
                     /locus_tag="ACHOTW_005355"
     CDS             complement(2546..2671)
                     /gene="ecnA"
                     /locus_tag="ACHOTW_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9847229.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2723..3289)
                     /gene="efp"
                     /locus_tag="ACHOTW_005356"
     CDS             complement(2723..3289)
                     /gene="efp"
                     /locus_tag="ACHOTW_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9847230.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3331..4359
                     /gene="epmB"
                     /locus_tag="ACHOTW_005357"
     CDS             3331..4359
                     /gene="epmB"
                     /locus_tag="ACHOTW_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP9847231.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            4594..5463
                     /locus_tag="ACHOTW_005358"
     CDS             4594..5463
                     /locus_tag="ACHOTW_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP9847232.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(5656..6009)
                     /locus_tag="ACHOTW_005359"
     CDS             complement(5656..6009)
                     /locus_tag="ACHOTW_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP9847233.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(6147..7793)
                     /gene="groL"
                     /locus_tag="ACHOTW_005360"
     CDS             complement(6147..7793)
                     /gene="groL"
                     /locus_tag="ACHOTW_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9847234.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(7837..8130)
                     /locus_tag="ACHOTW_005361"
     CDS             complement(7837..8130)
                     /locus_tag="ACHOTW_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9847235.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            8406..9662
                     /gene="yjeH"
                     /locus_tag="ACHOTW_005362"
     CDS             8406..9662
                     /gene="yjeH"
                     /locus_tag="ACHOTW_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP9847236.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(9678..10154)
                     /locus_tag="ACHOTW_005363"
     CDS             complement(9678..10154)
                     /locus_tag="ACHOTW_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP9847237.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            10491..11927
                     /gene="aspA"
                     /locus_tag="ACHOTW_005364"
     CDS             10491..11927
                     /gene="aspA"
                     /locus_tag="ACHOTW_005364"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP9847238.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            12045..13346
                     /gene="dcuA"
                     /locus_tag="ACHOTW_005365"
     CDS             12045..13346
                     /gene="dcuA"
                     /locus_tag="ACHOTW_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP9847239.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            13462..13800
                     /gene="cutA"
                     /locus_tag="ACHOTW_005366"
     CDS             13462..13800
                     /gene="cutA"
                     /locus_tag="ACHOTW_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP9847240.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            13776..15473
                     /gene="dsbD"
                     /locus_tag="ACHOTW_005367"
     CDS             13776..15473
                     /gene="dsbD"
                     /locus_tag="ACHOTW_005367"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP9847241.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLTIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            15510..16085
                     /locus_tag="ACHOTW_005368"
     CDS             15510..16085
                     /locus_tag="ACHOTW_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9847242.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         3952 a         4177 c         4014 g         4070 t
ORIGIN      
        1 ccggcccgcc tatggcgggc cgttttgtat ggaaaccaga ccctatgttc aaaacgacgc
       61 tctgcgcctt attaattacc gcctcttgct ccacatttgc tgcccctcaa caaatcaacg
      121 atattgtgca tcgcacaatt accccgctta tagagcaaca aaagatcccc ggtatggcgg
      181 tggcggtaat ttatcagggt aaaccttatt actttacctg ggggtatgcg gacatcgcca
      241 aaaagcagcc cgtcacacag caaacgttgt ttgagttagg ttcggtcagc aaaacattta
      301 cgggcgtgct tggtggcgac gctattgctc gaggggaaat caagttaagc gatcccgcaa
      361 caaaatactg gcctgaactt accgctaaac agtggaatgg gatcacacta ttacatctcg
      421 caacctacac tgctggcggc ctgccattgc aggtgccgga tgaagtgaaa tcctcaagcg
      481 acttgctgcg cttctatcaa aactggcagc ctgcatgggc tccaggaaca caacgtctgt
      541 atgccaactc cagtatcggt ttgttcggcg cactggctgt gaagccgtct ggtttgagtt
      601 ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa actcaaccat acgtggatta
      661 atgtaccgcc cgcagaagaa aagaattacg cctggggata tcgcgaaggc aaggcagttc
      721 atgtttcgcc aggggcgtta gatgctgaaa cttatggtgt gaagtcgacc attgaagata
      781 tggcctgctg ggtacgaagc aatatgaatc cccgtgatat caacgacaaa acacttcagc
      841 aagggataca actggcacaa tctcgctact ggcaaaccgg cgatatgtat cagggtctgg
      901 gctgggaaat gctggactgg ccggtaaatc ctgacatcat cgttaacggc agtgacaata
      961 aaattgcact ggcagcacgc cccgtaaaag cgattacgcc tccaactcct gcagtacgag
     1021 catcatgggt gcataaaacg ggggcgaccg gcggatttgg tagctatgtc gcgtttattc
     1081 cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa ctatcccaat ccagcgagag
     1141 tcgccgccgc ctggcagatt ctcaacgctc tacagtaaaa tttcatcggg tccgaatttt
     1201 cggacctttt ctgcgctttt ccttgctgtc atctacactt agaaaaaacc agtaaggaaa
     1261 catttatgcg cctgctccct ctcgttgccg cagcgacagc tgcatttctg gtcgttgcct
     1321 gcagttctcc tacgccgccg cgtggcgtga ccgtagtgaa taatttcgac gctaaacgct
     1381 atctcggtac ctggtatgag attgcccgtt ttgatcaccg ctttgaacgt ggactggaaa
     1441 aagtcaccgc aacatacagc ctgcgtgatg acggcggcct gaatgtcatt aataaaggct
     1501 ataaccctga cagaggaatg tggcagcaga gtgaagggaa agcgtacttt accggcgcac
     1561 caactcgcgc tgcgctgaaa gtgtcattct ttggtccttt ctatggcggt tataacgtta
     1621 ttgcactcga tcgggaatac cgccatgcgc tggtttgcgg accggaccgc gactacctgt
     1681 ggatactctc ccgcacgcca accatttctg acgaagtgaa acaggagatg ctggcagtcg
     1741 cgacccggga agggtttgat gtcagtaagt ttatttgggt acagcagccc ggtagttagt
     1801 gagtgctgag tttcagacca ataatcccca atacgattaa cgccagactc gccagacgca
     1861 tcgggttagc ggactcaccg agcagcacaa tgccggtgat ggccgcgccg actgcgccaa
     1921 tacccgtcca cacggcataa gccgtcccta ctggtaacga tttcatcgcc caggcaagta
     1981 gcgccatact gacaatcatc gccgtcacgg taataacact cggcgtcaaa cgactaaagc
     2041 cgtgggtata tttcaggcct acggcccata ccacttccag cagaccagca ataactaaga
     2101 taatccagga catatcaggc tccggagaat tggggccgtc cccggtgaaa agacgcgttt
     2161 gcaggtcgtc ctgcaaagct gatggttgaa cggatatttt gccaggtggc gggggaattt
     2221 caactattac ctttgtgact atcgcagaaa aaacaaacgg cacgacacag ctgtcgtacc
     2281 gttattgctt attgctgcgc tttcgttgct gcaccagaaa tcgcgttacc gccatcagaa
     2341 atgtcttcac caacgccacg cgtggtgttg caggcagtta atactgttga aagcaccaga
     2401 acagaaaaga tcgctgcaat tgtcttcttc accataacgt cttcctttta ggtaatgtta
     2461 tttatgtttg cctatagcaa attaataata gacaacatcg ccaaaaatga tggaaatctg
     2521 atgatttttc ggaagagaag aaaaattagc tggcagcgcg ggagatggag ttgccgagat
     2581 gtttgatgtc ttcgccgaaa ccgcgagcgg tgttacagcc cgtgagcagc gtgctggcaa
     2641 gcaaaacaag aacgataagg cgtttcatca tctctgacct gaataagtga tggtgcagcc
     2701 tgcaggccgc accacaaccg cattacttca cgcgagagac gtattcacca gagcgggtat
     2761 ccactttgat gacttcgccg atttgtacga acagcggaac tttaaccaca gcgccagtag
     2821 acagggtagc cggtttgccg ccagtacctg cggtatcacc tttcaggccc ggatcggtat
     2881 caacgatttc cagttcaacg aagttcggcg gagtaacgga gatcggctga ccattccaca
     2941 gagttacgat acactctgcc tgatccagca gccatttagc gttgtcgcca attgcttttg
     3001 cgtcagcaga cagctgctcg aaagtttcgt tgttcatgaa gtgccagaac tcaccgtcgt
     3061 tgtacaggta agtcaggttc atatcgacaa catcagcgcc ttcagcggaa tcagtagatt
     3121 tgaaggtttt ttctacgcga gtaccggtca gcagacgacg cagtttaacg cgagcaaatg
     3181 cctggccttt acccggtttt acgaattcac tcgcttcaac cgcgtaaggt tcgccgtcta
     3241 acatgatttt aagaccagca cgaaaatcgt tgctatagta cgttgccata aggccctctg
     3301 aaatttgtta attggtagct aagccacaaa atggcgcata ttgtaaccct aaatacccca
     3361 tccagagaag attggttaac gcaacttgcc gatgttgtga ccgatcctga tgaacttctg
     3421 cgtcttttga atatagacgc ggacgaaaaa ctgttggccg gacgcaacgc caaaaagctg
     3481 tttgccctgc gtgtgccccg ctcatttatc gatcgcatgg agaaaggcaa tccggacgat
     3541 ccgcttttgc gtcaggtact tacctcgcaa gatgagtttg tcgtcgcgcc cggattctcc
     3601 accgacccgc tggaagaaca gcacagcgta gtgcctggtt tgttgcataa ataccacaac
     3661 cgggcgcttt tgctggtcaa aggcggctgc gcggtaaatt gccgctattg cttccgtcgc
     3721 cacttcccct atgccgaaaa tcagggcaac aagcgtaact ggcaaactgc acttgagtat
     3781 gttgctgcgc atccggaact ggacgagatg attttctccg gcggcgatcc gctgatggcg
     3841 aaagatcacg agctggactg gttgctcaca caactggaag ccatcccgca tatcaaacgt
     3901 ctgcggattc acagtcgtct gccgattgtg atcccggcac gcatcacaga tgcgctggtt
     3961 gaacgctttt cacattctac gctgcaaatc ttgctggtga atcacatcaa ccatgccaat
     4021 gagatagatg aaacattccg tcaggcgatg gctaagttgc gccgggcggg cgttactttg
     4081 ctgaaccaga gcgttctgtt acgtggtgtg aacgataacg cacaaacgct ggcaaacctg
     4141 agtaatgcgt tgttcgatgc aggcgtgatg ccgtattacc tgcatgtgct cgataaagta
     4201 cagggcgcgg cgcattttat ggtgagtgat gacgaagcac ggcagattat gcgtgagttg
     4261 ctgacactgg tgtcgggata tctggtgccg aaactggcgc gagaaattgg cggcgaaccc
     4321 agcaaaacgc cgctggatct ccagctacgc cagcagtaac tgattaaaca gggaagttta
     4381 atctttgccc gttacataac ctttaattat tcatatacat tgctgttgtt actctttata
     4441 taaccaacac caataattaa cctttctttt ctgtttatat agagtgagta cggctattta
     4501 tgttatttta acaactatta ctaccaagcc catttatctg gcttataaat taaaattaat
     4561 tatttaattg agaaatggtt agggagaacc tacatggcca taagcatcaa gggtgttaat
     4621 accggtgtta ttcgcaagag taataatttc attgcactgg cgctgaaaat taaagaacct
     4681 cgcaataaag aatcgttgtt ctttatgtct gtcatggaac tgcgcgacct gctgattgcg
     4741 ctggaaagtc gtctgcatca aaagcataaa ctggatgctg ctgcacgcct tcagtatgag
     4801 caagctcgcg ataaagtcat taagaaaatg gcagaaaaca tcccagaaat tctggttgat
     4861 gagcttaaaa atgccgatat taatcgtcgg gttaacacgc tggaacttac cgataatcaa
     4921 ggcgaaaatc ttaccttcgt attaacgcta catgacggta gcaaatgtga gttagtcgtc
     4981 aatgagttgc agattgaaat gctggcgcga gcaatcattc atgcaattaa taatgcggaa
     5041 atgcgtgaac tggcattacg tattacctca ctgttagatt tcctgccgtt atatgacgtt
     5101 gactgccagc aaaatggcaa tctggaatat gacacctatt cccaaccgga atggaaacat
     5161 aacttgttcg accattatct ggcggtgctt taccgcttta aagatgaaag tggcaaagaa
     5221 caatttagcg gcgcagtggt gaaaacgcgt gaagcaacgc cgggtaaaga aatagaggcg
     5281 atcactcgcc gaatgcttga tttcagccca cgactgaaaa aactcgtggg tgtgccatgt
     5341 caggtctatg ttcgcaccgt agcggcgaat aacgcccaac cgctaaccca ggatcaatgc
     5401 ttacgcgcgc tgcatcactt acgtgttcaa tccaccagca aaactgcacc acaggcgaaa
     5461 taatctcatc tttgctaatg cgaggctgga gccatccagc ctgaaatgac tgataacgtg
     5521 atcgttggtc atgccggatg cggcgtaaac gccttatccg gcctacaaat tctcaagcat
     5581 tcaatcagtt gcaatgcctt atgtaggcct gataagcgaa gcgcatctgg caattatacg
     5641 tttgtcacca tcacatcagt tcgggcactt atatacctgg ccgataatct ggctatccgt
     5701 cgggacaaaa ctggacaaca ttccctgcga cgggctactg atgccataaa tcacgttacc
     5761 gcccattgca gccgcctggt tgcgcagatc gtttgctgcg ccgcgcatag aaccgccctc
     5821 ttctccgtgt tgcccggaaa gccagttgct ttgcttacct gtcgcagtac caatcagctg
     5881 gcactctgcg cctggctgct cgtccacaat gcgtacactc tgaccggcag cactcaattc
     5941 gttgctggag ctacaacccg ccatcagtag cgcggcaccg acaatccctg ctaagtattt
     6001 tacgtgcatg ttattcccca taatcaatga gctggacgcc actcgcgtcg tccgtgtctg
     6061 aatcttatac tagaaagatg accaaaagaa aaacccccag acatttctgc ccgggggttt
     6121 gtttatttct gcgaggtgca gggcaattac atcatgccgc ccatgccacc catgccaccc
     6181 atgccgccag cagcgcctaa gtcagctgca tcgtttttcg gcaggtcggt aaccatgcat
     6241 tcggtggtga tcatcaggcc agccacagaa gccgcgtact gcagagcaga acgggttact
     6301 ttggttgggt ccaggatacc catgtcgatc atgttgccgt attcttcggt tgctgcgttg
     6361 taaccgtagt tgccgtcgcc gcctttaacg gtgttagcaa caacagacgg ttcttcgccg
     6421 cagttcagga cgatctgacg cagcggagct tccattgcac gcagtgcaac tttgataccc
     6481 acgttctggt cttcgttctg accacgcagg tcagccagtt tagacgctac gcggatcagc
     6541 gcaacaccac caccagcaac cacgccttct tctaccgcag cacgggtcgc gtgcagggcg
     6601 tcttcaacgc gtgctttttt ctctttcatt tcaacttcgg tagcagcacc tactttgata
     6661 actgcaacgc cgcctgccag tttcgctacg cgctcctgca gtttttcacg gtcgtagtca
     6721 gaagttgctt cttcaatctg ctgacggatc tgagcaacac ggccctggat tgcagcttct
     6781 tcgcccacgc catcgatgat ggtggtggtg tctttgttga tcacaacgcg tttagcctga
     6841 cccaggtctt ccagggttgc tttttccagc tccataccga tctcttcaga gattacggta
     6901 ccgccagtca gggttgcgat atcctgcagc atagctttac gacgatcgcc gaagcccgga
     6961 gctttaactg cagcaacttt cacgatgcca cgcatggtgt taacaaccag agttgccagc
     7021 gcttcgcctt ctacatcttc agcgatgatc agcagcggtt tgcctgcttt ggcaacggct
     7081 tccagaaccg gcagcatttc gcggatgttg gagattttct tgtcagccag caggatgaac
     7141 gggctttcca gttctactgc gccagtttcc ggcttgttga tgaagtaagg agacaggtag
     7201 ccacggtcga actgcatacc ttcaaccacg tccagttcgt cctgcagacc ggtaccgtct
     7261 tcaacggtga taacgccttc tttaccgact ttgtccatcg cttcagcgat cagtttacct
     7321 acggtttcgt cggagttagc ggagatagta ccaacctgag caatcgcttt agagtcagag
     7381 cacggtacgg acagcgcttt cagttcttca actgcagcgg taacagcttt gtcgatacca
     7441 cgtttcaggt ccatcgggtt catgcccgca gcaacagctt tcagaccttc agtgatgata
     7501 gcctgagcca gtacggttgc agtggtggta ccgtcgcctg cagcgtcgtt cgctttagag
     7561 gcaacttctt tcaccatctg cgcacccatg ttttcgaact tgtcttccag ttcgatttca
     7621 cgagcaacgg aaacaccatc tttggtgatg gtcggtgcac cgaaagattt atccagaact
     7681 acgttacggc ctttcggacc gagggtaact ttcactgcat ctgccagtac gtttacgccg
     7741 cgcagcattt tcacacgagc gtcgttaccg aattttacgt ctttagctgc cattatcttt
     7801 attccttaaa ttcgtatgtt cagtgtcgtg cgaggattac gcttcaacaa ttgccagaat
     7861 gtcgctttcg gacatgatca acacttcttc attgtcgatc ttctcggatt tcacaccgta
     7921 gccatcgttg aaaataacga tgtcgccaac tttcacatcc agcggcttca cttcgccatt
     7981 ttcaaggata cggccattgc cgacagccag cacttcgccg cgggtggatt tagccgctgc
     8041 agagccggtc agaacgatgc cgccagcaga tttagtttca acttctttac gcttgacgat
     8101 cacgcgatca tgcaatggac gaatattcat tgataactct cctttgagaa agtccgtatc
     8161 tgttatgggt gacgccggag cttacgtggt ttcccggctg gtgaccagag aaatggggat
     8221 gaggcttcgc cccttcaagg gggaaaaaga aaaaaaattc tgatcagcac aaaatcgggt
     8281 gaaaaccctg attcacctca catttcatcg caatttcttc atcgccgtca taagcgaatc
     8341 tgattgtgct accatcgaaa atctacgcag ttgcccaaaa tttgggcgca atcggacatc
     8401 aacccatgag tggactcaaa caagaactgg ggctggccca gggcattggc ctgctatcga
     8461 cgtcattatt aggcactggc gtgtttgccg ttcctgcgtt agctgcactg gtagcgggca
     8521 ataacagcct gtgggcgtgg cccattttga ttatcttagt gttcccgatt gcgattgtgt
     8581 ttgcgattct gggtcgccac tatcccagcg caggcggcgt cgcgcacttc gtcggtatgg
     8641 cgtttggttc gcggcttgag cgagtcaccg gctggctgtt tttatcggtc attcccgtgg
     8701 gtttgcctgc cgcgctacaa attgctgccg gattcggcca ggcaatgttt ggctggcata
     8761 gctggcaact gttgttggca gaactcggta cgctggcgtt ggtgtggtat atcggtactc
     8821 gcggtgccag ttccagtgca aatctacaaa cagttattgc cggacttatc gtcgcactga
     8881 ttgtcgctat ctggtgggcg ggcgatatca aacctgcgag tatccccttc cccgcgccag
     8941 gaaatatcga acttaccggg ttatttgctg cgttatcagt gatgttctgg tgttttgtcg
     9001 gtctggaagc atttgcccat cttgcctcgg aatttaaaaa tccagaacgt gattttcctc
     9061 gtgctttgat gattggtctg ctgctggcag gattagtcta ctggggctgt acggtagtcg
     9121 tcttacactt cgacgcctat ggtgaacaaa tggccgcggc agcatcgctt ccaaaaattg
     9181 tagtacaact gttcggtgta ggagcgttat ggattgcctg cgtaattggc tatctggcct
     9241 gctttgccag tctcaacatt tatatacaga gcttcgcccg cctggtctgg tcgcaggcgc
     9301 aacataatcc tgaccactac ctggcacgcc tctcttctcg ccatatcccg aataatgccc
     9361 tcaatgcggt gctcggctgc tgcgtggtga gcacgttggt gattcatgct ttagagatca
     9421 atctggacgc tcttattatt tatgccaatg gcatctttat tatgatttat ctgttatgca
     9481 tgctggcagg ctgtaaatta ttgcaaggtc gttatcgact actggcggtg gttggcgggc
     9541 tgttatgcgt tctgttactg gcaatggtcg gctggaaaag tctctatgcg ctgatcatgc
     9601 tggcggggtt atggctgttt ctgccaaaac gaaaaacgcc ggaaaatggc ataaccacat
     9661 aatccggcgt ttcgacatta atcctggcga tcgtctttat gatcaaggcg gtcgcggtca
     9721 tcatcctttc gctggtactc accatcaaaa gtattaccgc caccggtccc ggcgctaaaa
     9781 ccgccgcctg gcatgcgaga aaagcgcaaa tgcggcatca acttcactgt cagatgcttt
     9841 tgcaccggcg gcaataaaag cagaagaccg aggaagtcgg tgaaaaagcc cggtaataaa
     9901 agcagcaaac cggcaatgat cagcgaaaca cttttaatca tctccgccgc tgggttttca
     9961 cccgccgcca ttttttgctg catcagcacg aaattcttaa agccctggtt acgtaccagt
    10021 gacataccga taactgacgt aaatataacc agcacgagag tcagcaatac ccccaataca
    10081 tgggcaacct gaataaagat tgaaatctca atatagacat aaaggaaaat ggcaataaaa
    10141 ggtaaccagc gcaaaggttt ctcctgtaat agcagccggt taaacccggc tacctgaatg
    10201 ggttgcgaat cgcgttcagc ttatattgtg gtcattagca aaatttcaag atatttgcgc
    10261 aactattttt ggtagtaatc ccaaagcggt gatctatttc acaaattaat aattaagggg
    10321 taaaaaccga cacttaaagt gatccagatt acggtagaaa tcctcaagca gcatatgatc
    10381 tcggatattc ggtcgatgca ggggataatc gtcggtcgaa aaacattcga aaccacatat
    10441 attctgtgtg tttaaagcaa atcattggca gcttgaaaaa gaaggttcac atgtcaaaca
    10501 acattcgtat cgaagaagat ctgttgggta ccagggaagt tccagctgat gcctactatg
    10561 gtgttcacac tctgagagcg attgaaaact tctatatcag caacaacaaa atcagtgata
    10621 ttcctgaatt tgttcgcggt atggtaatgg ttaaaaaagc cgcagctatg gcaaacaaag
    10681 agctgcaaac cattcctaaa agtgtagcga atgccatcat tgccgcatgt gatgaagtcc
    10741 tgaacaacgg aaaatgcatg gatcagttcc cggtagacgt ctaccagggc ggcgcaggta
    10801 cttccgtaaa catgaacacc aacgaagtgc tggccaatat cggtctggaa ctgatgggtc
    10861 accagaaagg tgaatatcag tacctgaacc cgaacgacca tgttaacaaa tgtcagtcca
    10921 ctaacgacgc ctacccgacc ggtttccgta tcgcagttta ctcttccctg attaagctgg
    10981 tagatgcgat taaccaattg cgtgaaggct ttgaacgtaa agcggtcgaa ttccaggaca
    11041 tcctgaaaat gggtcgtacc cagctgcagg acgcagtacc gatgaccctc ggtcaggaat
    11101 tccgcgcttt cagcatcctg ctgaaagaag aagtgaaaaa catccaacgt accgctgaac
    11161 tgctgctgga agttaacctt ggcgcaacag caatcggtac tggtctgaac acgccgaaag
    11221 agtactctcc gctggcagtg aaaaaactgg ctgaagttac tggcttccca tgcgtaccgg
    11281 ctgaagacct gatcgaagcg acctctgact gcggcgctta tgttatggtt cacggagcgc
    11341 tgaaacgcct ggctgtgaag atgtccaaaa tctgtaacga cctgcgcttg ctctcttctg
    11401 gtccacgtgc cggcctgaac gagatcaacc tgccggaact gcaggcgggc tcttccatca
    11461 tgccagctaa agtaaacccg gttgttccgg aagtggttaa ccaggtatgc ttcaaagtca
    11521 tcggtaacga caccactgtt accatggcag cagaagcagg tcagctgcag ttgaacgtta
    11581 tggagccggt cattggccag gctatgttcg aatccgttca cattctgacc aacgcttgct
    11641 acaacctgct ggaaaaatgc attaacggca tcactgctaa caaagaagtg tgcgaaggtt
    11701 acgtttacaa ctctatcggt atcgttactt acctgaaccc gttcatcggt caccacaacg
    11761 gtgacatcgt gggtaaaatc tgtgccgaaa ccggtaagag tgtacgtgaa gtcgttctgg
    11821 aacgcggtct gttgactgaa gcggaacttg acgatatttt ctccgtacag aatctgatgc
    11881 acccggctta caaagcaaaa cgctatactg atgaaagcga acagtaatcg tacagggtag
    11941 tacaaataaa aaaggcacgt cagatgacgt gccttttttc ttgtgagcag taacttaaaa
    12001 ataacaatct aatatcaact tgttaaaaaa caaggaaggc taatatgcta gttgtagaac
    12061 tcatcatagt tttgctggcg atcttcttgg gcgccagatt ggggggaata ggtattggtt
    12121 ttgcaggcgg attgggggtg ctggttcttg ccgctattgg cgttaaaccc ggtaacatcc
    12181 cgttcgatgt catttccatt atcatggcgg ttatcgccgc tatttctgcc atgcaggttg
    12241 ctggcggtct ggactatctg gttcatcaga cagaaaagct gctgcgccgt aacccgaaat
    12301 acatcacgat cctcgcaccg atcgtgacct atttcctgac tatctttgct ggtactggca
    12361 acatctctct ggcgacactg ccagttatcg ctgaagttgc gaaggaacaa ggcgttaaac
    12421 cttgccgtcc gctgtctact gcagtggtat ccgcgcagat tgcgatcacc gcatcgccaa
    12481 tctcagcggc agtggtttac atgtcttccg tgatggaagg tcatggcatc agctacctcc
    12541 atctgctctc cgtggtcatc ccgtccaccc tgctggcggt tctggtgatg tccttcctgg
    12601 tcactatgct gttcaactcc aaactctctg acgatccgat ttatcgcaag cgtctggaag
    12661 agggcctggt tgaactgcgc ggtgaaaagc agattgaaat caaatccggt gcaaaaacgt
    12721 ccgtctggct gttcctgctg ggcgtagttg gcgtggttat ctatgcaatc atcaacagcc
    12781 caagcatggg tctggttgaa aaaccgctga tgaacaccac caacgcaatc ctgatcatca
    12841 tgctcagtgt tgcaactctg accaccgtta tctgtaaagt cgataccgac aacatcctca
    12901 actccagcac cttcaaagca ggtatgagcg cctgtatttg tatcctgggt gttgcgtggc
    12961 tgggcgatac tttcgtttcc aacaacatcg actggatcaa agataccgct ggtgaagtga
    13021 ttcagggtca tccgtggctg ctggccgtca tcttcttctt tgcttctgct ctgctgtact
    13081 ctcaggctgc aaccgcaaaa gcactgatgc cgatggctct ggcactgaac gtttctccgc
    13141 tgaccgctgt tgcttctttc gctgcggtgt ctggtctgtt cattctgccg acctacccga
    13201 cgctggttgc tgcggtacag atggatgaca cgggtactac ccgtatcggt aaattcgtct
    13261 tcaaccatcc gttcttcatc ccgggtactc tgggtgttgc cctggccgtt tgcttcggct
    13321 tcgtgctggg tagcttcatg ctgtaatgac caatcgcggg gcgttcacgc cccgctttct
    13381 ttctcgccga ctaacatcct ttccccgtcc gttgtatagt gacctctctc ttgcggttcc
    13441 atctgttctt gcgaggtgtt tatgcttgat gaaaaaagtt cgaataccgc gtctgtcgtg
    13501 gtgctatgta ccgcaccgga tgaagcgaca gcccaggatt tagccgccaa agtgctggcg
    13561 gaaaaactgg cggcctgcgc gaccttgatc cccggcgcta cctctctcta ttactgggaa
    13621 ggtaagctgg agcaagaata cgaagtgcag atgattttaa aaactaccgt atctcaccag
    13681 caggcactgc tggaatgcct gaagtctcat catccatatc aaaccccgga acttctggtt
    13741 ttacctgtta cacacggaga cacagattac ctctcatggc tcaacgcatc tttacgctga
    13801 tcctgctact ttgcagcact tccgtttttg ccggattatt cgacgcgccg ggacgttcac
    13861 aatttgtccc cgtggatcaa gcctttgctt ttgattttca gcaaaaccaa catgacctta
    13921 atctgacctg gcagatcaaa gacggttact acctctaccg taaacagatc cgcattacgc
    13981 cggaacacgc gaaaattgcc gacgtgcagc tgccgcaagg cgtctggcat gaagatgagt
    14041 tttacggcaa aagcgagatt taccgcgatc ggctgacgct tcccgtaacc atcaaccagg
    14101 cgagtgcggg agcgacgtta actgtcacct accagggctg tgctgatgcc ggtttctgtt
    14161 atccgccaga aaccaaaacc gttccgttaa gcgaagtggt cgccaacaac gcagcgtcac
    14221 agcctgtgtc tgttccgcag caagagcagc ccaccgcgca attgcccttt tccgcgctct
    14281 gggcgttgtt gatcggtatt ggtatcgcct ttacgccatg cgtgctgcca atgtacccac
    14341 tgatttctgg catcgtgctg ggtggtaaac agcggctctc cactgccaga gcattgttgc
    14401 tgacctttat ttatgtgcag gggatggcac tgacttacac ggcgctgggt ctggtagttg
    14461 ccgccgcagg gttacagttc caggcggcgc tacagcaccc atacgtgctc attggcctca
    14521 ccatcgtctt taccttgctg gcgatgtcaa tgtttggctt gcttactcta caactcccct
    14581 cctcgctgca aacgcgcctc acgctgatga gcaatcgcca acagggcggc tcacccggcg
    14641 gagtgtttat tatggggacg attgccggac tgatctgttc accatgcacc accgcaccgc
    14701 ttagcgcgat tctgctgtat atcgcccaaa gcgggaacat gtggctgggc ggcggcacgc
    14761 tttatctcta tgcgttgggc atgggcctgc cgctgatgct aattaccgtc tttggtaacc
    14821 gcttgctgcc gaaaagcggc ccgtggatgg aacaagtcaa aaccgcgttt ggttttgtga
    14881 tcctcgcact gccggtcttc ctgctggagc gagtgattgg tgatgtatgg ggattacgct
    14941 tgtggtcggc gctgggtgtc gcattctttg gctgggcctt tatcaccagc ctacaggcca
    15001 aacgcggctg gatgcgcgtg gtgcaaataa tcctgcttgc agcggcattg gttagcgtgc
    15061 gcccacttca ggattgggca tttggtgcga cgcataccgc gcaaactcag acgcatctca
    15121 actttacaca aatcaaaacg gtagatgagt taaatcaggc gctcgttgaa gccaaaggca
    15181 aaccggtgat gttagatctt tatgccgact ggtgcgtcgc ctgtaaagag tttgagaaat
    15241 acaccttcag cgacccgcag gtgcaaaaag cgttagcaga cacggtatta cttcaggcca
    15301 acgtcactgc caacgacgca caagatgtgg cgctgttaaa gcatcttaat gtccttggcc
    15361 taccgacaat tctctttttt gacggacaag gccaggagca tccacaagca cgcgtcacgg
    15421 gctttatgga tgctgaaacc ttcagcgcac atttgcgcga tcgccaaccg tgaacgacac
    15481 ttgcagtggg aaagacggag gaaaatagcg tgcaacgtga agatgtactg ggagaagccc
    15541 tgaaattatt agaattacaa gggattgcca acaccacgct ggagatggtt gctgaacgtg
    15601 tggattatcc actggacgag ctacgccgct tctggccaga caaagaggca atcctctacg
    15661 atgcgctgcg ttatcttagc caacagatag atgtctggcg tcgtcagctg atgctggacg
    15721 aaacgcaaac cgccgaacaa aagctgctgg cacgttatca ggcgttatcg gagtgcgtta
    15781 aaaacaaccg ctatccgggc tgtctgttta tcgctgcctg tacgttttat cccgatcctg
    15841 gccaccctat tcatcaactg gccgatcagc aaaaaagcgc ggcctacgat ttcacccacg
    15901 aactgttaac cacgctggaa gttgacgatc cggcgatggt agcaaagcag atggaactgg
    15961 tgctggaagg ctgtttaagc cgaatgctgg tgaatcgtag ccaggcggat gtcgacaccg
    16021 cacatcggct ggcggaagat atcctgcgct tcgcccgctg ccgccagggt ggtgcactga
    16081 cctgacagaa acacagaaaa gaagcgattt gccgcaatct taagcagttg aatcgctttt
    16141 actgaaatta ggttgacgag atgtgcagat tacggtttaa tgcgccccgt tgcccggata
    16201 gctcagtcgg tag
//
LOCUS       DAZKIF010000544         5567 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.guided.218, whole genome shotgun sequence.
ACCESSION   DAZKIF010000544 DAZKIF010000000
VERSION     DAZKIF010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.guided.218"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            99..302
                     /locus_tag="ACHOTW_005369"
                     /pseudo
     CDS             99..302
                     /locus_tag="ACHOTW_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            301..822
                     /gene="prfH"
                     /locus_tag="ACHOTW_005370"
                     /pseudo
     CDS             301..822
                     /gene="prfH"
                     /locus_tag="ACHOTW_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHOTW_005371"
     CDS             complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHOTW_005371"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9847243.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2597..3055
                     /gene="gpt"
                     /locus_tag="ACHOTW_005372"
     CDS             2597..3055
                     /gene="gpt"
                     /locus_tag="ACHOTW_005372"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9847244.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3147..4391
                     /gene="frsA"
                     /locus_tag="ACHOTW_005373"
     CDS             3147..4391
                     /gene="frsA"
                     /locus_tag="ACHOTW_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9847245.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4449..4850
                     /gene="crl"
                     /locus_tag="ACHOTW_005374"
     CDS             4449..4850
                     /gene="crl"
                     /locus_tag="ACHOTW_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9847246.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4889..>5567)
                     /locus_tag="ACHOTW_005375"
     CDS             complement(4889..>5567)
                     /locus_tag="ACHOTW_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP9847247.1"
                     /translation="DMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTL
                     QYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRA
                     EAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGY
                     VLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVA
                     VGMTYQF"
BASE COUNT         1346 a         1376 c         1449 g         1396 t
ORIGIN      
        1 tgaatcccca ggagcttaca taagtaagtg actggggtga acgaacgcag tcgcagtaca
       61 tgcaacttga agtatgacga gtataaatgg aatggtacat gggcaaatat attcgtccct
      121 tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt ttagcgatcc
      181 aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg atgccagatt
      241 tacaccccgg acgcggctac ccgattggcg cagcgttctt ctctgttggt cgtttttacc
      301 cgacaagacg tcgcggtaac ggtgctggaa acagaaacgg gccgctactc tgacacgctg
      361 cgttcggcgc tgatttctct ggatggcgac aacgcatggg cgttaagcga aagttggtgc
      421 ggcactattc agtggatttg tctgagtccg tatcggcctc atcatgggcg caaaaactgg
      481 tttctgggca ttgggcgttt taccgctgat gagcaggaac aatcggatgc aatccgttat
      541 gagacgctgc gtccgtcggg gccgggcggt caacatgtca ataaaaccga ctcggcggta
      601 cgcgccacgc atctggcaac cgggattagc gtgaaggttc agtcagaacg cagccagcat
      661 gctaacaaac ggctggcgcg attgctgatt gcctggaagc tggagcagca gcaacaggaa
      721 aatagcgcag tgctgaaatc gcagcggcga atgttccatc accagattga acgtggcaac
      781 ccgcgacgaa cgttcacagg gatggctttt atcgaaggat aatgaaggat gaaactgccg
      841 gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc
      901 agcagttcag tcagcagtgt ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc
      961 ggagagtgtg gaccggtgat tgttggcccg atagaaacca tgtccatttc cggatacggt
     1021 tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg cgtcttgttg
     1081 aacaggcgct gataggtttc acgtaccaga tgcatcaccg gagaattagc gtccggctgc
     1141 cagccaggat atgcgccttt cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc
     1201 agcatgctca ccacgtagtc tttaccgctg tcgatcagag aacggatcag acagtgaatt
     1261 tctacgttat tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct
     1321 tttgccacgt cggagttgcg gatcacaccg ttcggggtgg cgttcagcag acgaataaag
     1381 gtatcgcgag atttcgcaat cagggcagct ttatcgttcg ctacagagtc cagcaacaag
     1441 gccagatttt tctctttctc tgccagctcg tttttcagga tctcctgata ggtattcacc
     1501 agagatttca gggcgtcgac tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc
     1561 gggatggcgt tacgcagtgt gccgccgttg aaatcaataa gacgcaggtc cagttcttct
     1621 gcatgacccg ccaggaagcg caccagcagt ttgttggcat tacccaggcc aacgtggatt
     1681 tccccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca
     1741 gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc ccccgcgcaa
     1801 cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac
     1861 cagttgctct gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc
     1921 acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagtgc agaggccata
     1981 ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt taacccattc gccatcaata
     2041 tatggctgga taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc
     2101 atatcgaggt gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt
     2161 ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc
     2221 caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag
     2281 attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta
     2341 agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt
     2401 tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     2461 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     2521 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     2581 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     2641 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     2701 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     2761 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     2821 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     2881 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa
     2941 accggctggt cgtccgctgg ttgatgatta tgttgttgat atcccgcaag atacctggat
     3001 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     3061 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgagtta caatagtttc
     3121 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     3181 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     3241 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     3301 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     3361 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     3421 accgtggcgg caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag
     3481 cctgtgcgga agaagatccg caactcagtg gtcgtcactg gctgcatgcg gctacgttgt
     3541 acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
     3601 tgtcaaaccg cgcctatgaa gaggccgctc agcgtctacc gggcacgatg cgacagatgg
     3661 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
     3721 gcccgttccc gacggtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
     3781 gcctgtatga acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt
     3841 cggtgggctt ttcttcaaaa tggaagctca cccaggactc ctgcctgttg catcagcgcg
     3901 tcttaaaggc tctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
     3961 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag
     4021 cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
     4081 aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg
     4141 acgaagcgtt gcgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctgggac
     4201 gtcgatgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
     4261 aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta
     4321 acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccgactgg atcgaaaaac
     4381 gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg
     4441 agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
     4501 tactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
     4561 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga
     4621 tggagcttga agcgcaggaa tcccgtttta cctacagtta ccagtttggt ctgttcgata
     4681 aagcaggcga ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc
     4741 acaccctgcg tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagt
     4801 tggaaccggc ggatgatttt cgtgacgagc cggtgaagtt aacggcgtga gtgaaatgtg
     4861 ccggatgcat cacatccggc aataatcatt aaaactgata ggtcatgcca accgcgacaa
     4921 tatcatcatt attaatattc aatttgttat cgctatccag ttggttgatt ttataatcaa
     4981 caaacgctga catatttttg ttgaaataat atgtagcccc gacatcgata taattgacca
     5041 gatcttcatc accgatacct tcaatatctt tccctttcga taagacataa cccagcgatg
     5101 gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg
     5161 caaagccgcc agttattggc gtcattttgc gtgtttcaga ataaaaagtt gccagataaa
     5221 tattattggc atcgtatttc agacctgtag cccaagcttc tgcacgcttg cctgtgccac
     5281 ggctttgcag gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga
     5341 aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt
     5401 taacgtcgcg gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa
     5461 cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt
     5521 tgtcggtctg cgccgaggag tcgccaccaa attccgggaa catatcg
//
LOCUS       DAZKIF010000545         1992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.guided.318, whole genome shotgun sequence.
ACCESSION   DAZKIF010000545 DAZKIF010000000
VERSION     DAZKIF010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.guided.318"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            49..125
                     /locus_tag="ACHOTW_005376"
     tRNA            49..125
                     /locus_tag="ACHOTW_005376"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83..85,aa:Arg,seq:tcg)
     gene            139..215
                     /locus_tag="ACHOTW_005377"
     tRNA            139..215
                     /locus_tag="ACHOTW_005377"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:173..175,aa:Arg,seq:tct)
     gene            306..1265
                     /locus_tag="ACHOTW_005378"
     CDS             306..1265
                     /locus_tag="ACHOTW_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFP9847248.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1277..1546
                     /locus_tag="ACHOTW_005379"
     CDS             1277..1546
                     /locus_tag="ACHOTW_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFP9847249.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          515 a          431 c          494 g          552 t
ORIGIN      
        1 atcgccaggt cgctggttca aatccagcaa gggccaccat atcacatacc gccattagct
       61 catcgggaca gagcgccagc cttcgaagct ggctgcgcgg ggttcgagtc ctcgatggcg
      121 gtccattatc tgcattatgc gttgttagct cagccggaca gagcaattgc cttctgagca
      181 atcggtcact ggttcgaatc cagtacaacg cgccatattt atttaccagg ctcgcttttg
      241 cgggcctttt ttatatctgc gccgggtctg gtgctgatta cttcagccaa aaggaacacc
      301 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt ttttcttcgg
      361 tatcctattc ccgggagttt acgatagact tttcgaccca acaaagttat gtctcttcgt
      421 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      481 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      541 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      601 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      661 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      721 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      781 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      841 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      901 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      961 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
     1021 gtgtcagagt ggggagaata tcctttaata atatatcagc gatactgggg actgtggccg
     1081 ttatactgaa ttgccatcat cagggggcgc gttctgttcg cgccgtgaat gaagagagtc
     1141 aaccagaatg tcagataact ggcgacaggc ctgttataaa aataaacaat acattatggg
     1201 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
     1261 aataaaggag ttaagcatga agaagatgtt tatggcggtt ttatttgcat tagcttctgt
     1321 taatgcaatg gcggcggatt gtgctaaagg taaaattgag ttttccaagt ataatgagga
     1381 tgacacattt acagtgaagg ttgacgggaa agaatactgg accagtcgct ggaatctgca
     1441 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1501 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1561 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1621 tcagactgag catttgttaa aaaaatttcg catggtgaat ccccctgtgt ggaggggcga
     1681 ctggtgaaaa atccttgctt gtgattcatt atcgacacgg gttcggtggt accaggccga
     1741 actcaccggg aggcacccgg caccatgcag tatacagaga ttaggcatat accaaggcct
     1801 ctcatagcag gggccttttt acatgtaaaa aagcccgagt gggttcgggc aattgcatga
     1861 gatactcgtt ttaataatcg aaatcatttt aaccaggatt cataaggctg cgcaactgcg
     1921 cggccttttt cgtatttcgg gctgtagtct tccttctgcc attgtcctgt aacttccgga
     1981 cttcagcccg ct
//
LOCUS       DAZKIF010000546         1585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2204M51205
            SAMN43267872-rid23081733.guided.335, whole genome shotgun sequence.
ACCESSION   DAZKIF010000546 DAZKIF010000000
VERSION     DAZKIF010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267872
            Sequence Read Archive: SRR30307216
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350534.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:12:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,382
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 95 of 231
            Pseudo Genes (incomplete)         :: 119 of 231
            Pseudo Genes (internal stop)      :: 66 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267872-rid23081733.guided.335"
                     /serotype="O157:H 7"
                     /isolate="2204M51205"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            111..1058
                     /gene="stxA1a"
                     /locus_tag="ACHOTW_005380"
     CDS             111..1058
                     /gene="stxA1a"
                     /locus_tag="ACHOTW_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP9847250.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1068..1337
                     /gene="stxB1a"
                     /locus_tag="ACHOTW_005381"
     CDS             1068..1337
                     /gene="stxB1a"
                     /locus_tag="ACHOTW_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP9847251.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            complement(1398..>1585)
                     /locus_tag="ACHOTW_005382"
     CDS             complement(1398..>1585)
                     /locus_tag="ACHOTW_005382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9847252.1"
                     /translation="LCAIAWCRVPPGEFGLVPPNPRILAYDHQRDHTVHQSPLRTGGF
                     TMQKFSNTSIIRPATTD"
BASE COUNT          428 a          272 c          398 g          487 t
ORIGIN      
        1 cattacgtta ttgttacgtt tatccggtgc gccgtaaaac gccgtccttc agggggtgga
       61 ggatgtcaag aatatagtta tcgtatggtg ctcaaggagt attgtgtaat atgaaaataa
      121 ttatttttag agtgctaact tttttctttg ttatcttttc agttaatgtg gttgcgaagg
      181 aatttacctt agacttctcg actgcaaaga cgtatgtaga ttcgctgaat gtcattcgct
      241 ctgcaatagg tactccatta cagactattt catcaggagg tacgtcttta ctgatgattg
      301 atagtggcac aggggataat ttgtttgcag ttgatgtcag agggatagat ccagaggaag
      361 ggcggtttaa taatctacgg cttattgttg aacgaaataa tttatatgtg acaggatttg
      421 ttaacaggac aaataatgtt ttttatcgct ttgctgattt ttcacatgtt acctttccag
      481 gtacaacagc ggttacattg tctggtgaca gtagctatac cacgttacag cgtgttgcag
      541 ggatcagtcg tacggggatg cagataaatc gccattcgtt gactacttct tatctggatt
      601 taatgtcgca tagtggaacc tcactgacgc agtctgtggc aagagcgatg ttacggtttg
      661 ttactgtgac agctgaagct ttacgttttc ggcaaataca gaggggattt cgtacaacac
      721 tggatgatct cagtgggcgt tcttatgtaa tgactgctga agatgttgat cttacattga
      781 actggggaag gttgagtagt gtcctgcctg attatcatgg acaagactct gttcgtgtag
      841 gaagaatttc ttttggaagc attaatgcaa ttctgggaag cgtggcatta atactgaatt
      901 gtcatcatca tgcatcgcga gttgccagaa tggcatctga tgagtttcct tctatgtgtc
      961 cggcagatgg aagagtccgt gggattacgc acaataaaat attgtgggat tcatccactc
     1021 tgggggcaat tctgatgcgc agaactatta gcagttgagg gggtaaaatg aaaaaaacat
     1081 tattaatagc tgcatcgctt tcattttttt cagcaagtgc gctggcgacg cctgattgtg
     1141 taactggaaa ggtggagtat acaaaatata atgatgacga tacctttaca gttaaagtgg
     1201 gtgataaaga attatttacc aacagatgga atcttcagtc tcttcttctc agtgcgcaaa
     1261 ttacggggat gactgtaacc attaaaacta atgcctgtca taatggaggg ggattcagcg
     1321 aagttatttt tcgttgactt agaatagctc agtgaaaata gcaggcggag attcataaat
     1381 gttaaataca tctcaattca gtcagttgtt gccggtctga taatagatgt gttagaaaat
     1441 ttctgcatgg tgaatccccc tgtgcggagg ggcgactggt gaacggtatg atctctttga
     1501 tgatcgtaag cgagaatacg cgggtttggt ggcaccaggc cgaactcacc gggaggcacc
     1561 cggcaccatg caatggcaca tagcg
//
